LOCUS       DAOQDS010000001       116011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_44_length_116011_cov_23.269637, whole genome shotgun sequence.
ACCESSION   DAOQDS010000001 DAOQDS010000000
VERSION     DAOQDS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 116011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 116011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..116011
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44_length_116011_cov_23.269637"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1007
                     /locus_tag="PK589_00005"
     CDS             45..1007
                     /locus_tag="PK589_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ18003.1"
                     /translation="MRSHIKVQMGIGVLLVAGATSFAIAADASNENKDMVLVPKGEFI
                     MGSNEHSDEVRHQVVLDAYLIDKFEASNARYKEFMRATGHPAPAYWDDPRLSKPEQPV
                     VGVSWTDANAFCKWDGKRLPTEAEWEKAAKGPEGDNHYPWGHHLDPKKANYGQNVGRT
                     MPVDSYPEGVSGYGVYNMAGNVFEWVDDWYDPKYYKTSNALNPKGADKGYNFANQGPV
                     KVLRGGSWLAPETSLHTSHRFWNQPENNSYGVGLGFRCAKSVTAVSEEAAQAGRDAFI
                     QALVGMGAEKNADAMASIEKALAADPNNKEYLATRELIQKSMKK"
     gene            1074..2132
                     /locus_tag="PK589_00010"
     CDS             1074..2132
                     /locus_tag="PK589_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ18004.1"
                     /translation="MTVMGTVVRAGLVMGIIAGASVAQAHEAEIPKDMVYVGQGPSIM
                     GLDRTEAAAPTRRLSLYDKRMSMPWSAEAFHDEGPAHTVFLDSYLIDKYEVSNKDYGE
                     FIKATGHPAPAYWDDPRLNKPQQPVVGVNWIDAKTYCEYRGKRLPTEAEWEKAARGPN
                     GNLYPWGNDFDSNKANYDRHHNATQPVDAHPEGASYYGVYNMAGNVFEWVGDWYDPKY
                     YGRLETMVNPTGPEKPLWLGGTGTYVDRLTVGEKRVIRGGSWIAPEGTIRSTHRFWNH
                     PLNNSYGVGLGFRCAKTAPPDWEQRIRDSYITALVEMGRERFAEAQQAVARGLAMDPV
                     NVELLELRPLIEQSMKRP"
     gene            2186..2554
                     /locus_tag="PK589_00015"
     CDS             2186..2554
                     /locus_tag="PK589_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18005.1"
                     /translation="MRHRTIVTIIGGLAALVSAMELQAGPIDASRHTHPEKVQLVHEA
                     EHSVDHAWEVYHRAALGGTVASPDLQAQIEQHLHEARTLVTKAQEAADRGDAGAVEKL
                     VAQIKTHTERAIEGSKEQKK"
     gene            2551..3414
                     /locus_tag="PK589_00020"
     CDS             2551..3414
                     /locus_tag="PK589_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ18006.1"
                     /translation="MTQGLTRRRTRWGYAGLLAAASTLALSPVFATPAVKELDPVLMV
                     TIPAGPFLMGTQSPKGRADEWPQRTVQVDAFAMDEVEVTNERYMTFVTTTGHRSPPNP
                     YGTGVLVSAKGIEQLPVVQVTWYDAKAYCAWAKKRLPTEAEWEKAARGTDGRTFPWGE
                     DTPTSKRANYDREWVEDKTLHPVGALPAGDSPYGIKDMSGNAREWVQDWYDPDYYANA
                     PTKNPQGPDKGIVRVIRGGSWHSPHSDITSTARGRGGFALQTHGTGFRCVRSLDGQAH
                     QDASGAEPKAR"
     gene            3598..3729
                     /locus_tag="PK589_00025"
     CDS             3598..3729
                     /locus_tag="PK589_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18007.1"
                     /translation="MTQEMILGMAAFVVSIGAVLYVRLRRPAYVMVRRSSREHPRVR"
     gene            3906..4178
                     /locus_tag="PK589_00030"
     CDS             3906..4178
                     /locus_tag="PK589_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18008.1"
                     /translation="MTQHYIDLLDDTGQIDITAWHCAMCGEVLDPVILKNRHSPPPNL
                     LYGTKERKFSQRVTAVSNGGASKKSSHREAAPDKGKDSETGADRAP"
     gene            4209..5126
                     /locus_tag="PK589_00035"
     CDS             4209..5126
                     /locus_tag="PK589_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNJ18009.1"
                     /translation="MNIVVTGGTGFIGHALVEELSHAGHDVVVLSRRPHSPTQSKVRY
                     VEWDARSVGPWQDAVAAADAVVNLAGEPIAEGRWTSARKRILFESRILATRSLVDAIA
                     TRSRPLPVLISASGIGYYGLQDDRVLDERSPLGEGFLAQLSAAWEAEALRAGQFGTRV
                     VLLRTGMVLERDGGALPKMMLPFRLFAGGPVLPGTQWVSWIHRADLIGLMQWALHSPE
                     VSGPVNAVAPGPETMKAFCGILGKVLHRPSWLPVPGLALRLGLGELGTLMTTGQRVDP
                     AKAQAGGYRFRYPTLEPALRAIMTKEQRA"
     gene            5123..5599
                     /locus_tag="PK589_00040"
     CDS             5123..5599
                     /locus_tag="PK589_00040"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNJ18010.1"
                     /translation="MSLLVESIMQYLHGLAAAILVGKVVLLSFVVAPVLAKQLAPEQF
                     GKVVRQLFPAYYLLGMGAAAGGLVSVTALLVIRGASPGLSAAGGIWLIIFLAEAYCRS
                     PLTPQSNAMRDQLKEQESRGSVDQVLKASWERLHQRSLYLNSLVLLGGLCLVGLAS"
     gene            5624..>5817
                     /locus_tag="PK589_00045"
     CDS             5624..>5817
                     /locus_tag="PK589_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18011.1"
                     /translation="MMRKHTYRVAWMTLGVLSLCLSQWPEGTGKSWAAEGHEKSGTPG
                     GSYGLEVTIPDGVIGVSLHVG"
     assembly_gap    5818..5917
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5918..6264
                     /locus_tag="PK589_00050"
     CDS             <5918..6264
                     /locus_tag="PK589_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNJ18012.1"
                     /translation="GLEVTIPDGVIGVSLHVGAERVGDTAVLYIAQVLPDGPAEKAGL
                     KSGDEITTVDGAVVTGKTYEQVALMVRGEPGTIVRLGIKAETAVREVAVTRVAGQTLY
                     KGQMGSHGGSAK"
     gene            6272..6796
                     /locus_tag="PK589_00055"
     CDS             6272..6796
                     /locus_tag="PK589_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18013.1"
                     /translation="MKRMIALLGCFVMISGVCLTEVRANPTPASTLSGVEKTGLYLLA
                     GDYRRALESCEQALQERPSVEAYVRLTYVYQAIDAYLEQLSKDESWMAVEHLYLNLAY
                     RHTEDLVDPPGGLARMAKEMIQTSVRQQSDVSAAMAVRLNKVASDRLWQEQTQWRQAH
                     PTTWWQAFPDAWVQ"
     gene            6846..7277
                     /locus_tag="PK589_00060"
     CDS             6846..7277
                     /locus_tag="PK589_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18014.1"
                     /translation="MIQQTSQRGIDIKLFGALFLLVGLLDVLIIEWFPAYALKLFGVT
                     ITGPMAYVVKLHSPAAHFLIGYGFIFLRPWAWGLAMAYGGFGLTSELMNQWQFGFHQL
                     RTGFMATTALFLCYVAWRRVLFVDPLSPDTRLASSSREVHL"
     gene            7274..8713
                     /locus_tag="PK589_00065"
     CDS             7274..8713
                     /locus_tag="PK589_00065"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNJ18015.1"
                     /translation="MRGLVWFRRDLRLHDNPALSAACKECKEIVPLFVFDDPLLASHV
                     FGSVCVGFMLGCLEDLRRSLAEQGVTLVWRMGEPIDTVLRTVQECAVDAVYWNRDYEP
                     AAVARDRTVQQRLAQQGRLVRTFKDHVVFEAEEVRGLTGDPFQRYSAYRDRWWTQWRS
                     AAPPLLSVPSFPAASTAMKVSAWPTVQELGYESVPMWIEPGEQAARARLQWFLKKPVH
                     SYVSGRNLPAQDGTSKLSPHLRFGTISTRTMVHAALQTLSKGGQVSRPDVFTWMDELV
                     WREFFQQVLMAFPHVTEGPFKVKPGLPAPRPAGPERDRLFAAWCEGRTGYPIVDAGMR
                     QLNQTGWMHNRVRMVVASFLVKDLRIDWQSGERYFMQHLVDGDVAANNGNWQWCASTG
                     TDAMQGYRIFNPTLQSEKFDPDGHYLRCYVPELTSVPTKWIHEPQLMPQEEQIRVGCR
                     IGSDYPEPIVNHRQARQEYLDLGKQKVST"
     gene            8710..9663
                     /locus_tag="PK589_00070"
     CDS             8710..9663
                     /locus_tag="PK589_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HNJ18016.1"
                     /translation="MTAATLRLGISRCLLGEEVRFDGGHKRDAFLTDVLGRYVEWVPV
                     CPEVEAGLGTPREAMRLIGNPQQPRLVTLKSGQDHTRALEVMADRRLDELAGADLSGF
                     VFKKDSPSCGVERVRTYNEHGMPGRKGVGLFARAFLGRFPLIPVEEEGRLCDPLLREN
                     FIERVFCYRRWRDLMNSGATRQAVVRFHTIHKYLLLAHSAPHYRQLGRLVAQVSEYRP
                     KDLALQYGELFMQALAVRATVRKHVNVLHHILGHFKERLDAQEKAELLGVIGDYHQGL
                     TPLIVPLTLIKHYVQLFDVEYIREQVYLNPHPKELMLRNHV"
     gene            9674..10042
                     /locus_tag="PK589_00075"
     CDS             9674..10042
                     /locus_tag="PK589_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18017.1"
                     /translation="MPHVVVEGAGDLQSLYQNFAPMLQRADQEILKVQEFYLAKSGRE
                     ALLDAVVIEQGVARSFFIQLKQHDTTITVRLLPATDPEKTAAVKRVMALVAKFIRTVC
                     PASRYGKTNLQEFLEASVAK"
     gene            10081..10590
                     /locus_tag="PK589_00080"
     CDS             10081..10590
                     /locus_tag="PK589_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HNJ18018.1"
                     /translation="MATVRKGVILVGHGGIPRGCPQDMVTKLKRLEAQRRAAKQPASA
                     EEMELDAKIRQWPRNAETDPYQAGLEAVAAQLRAKLGDVLFAVAYNEFCAPTLEESVE
                     QLAKQGATHITVATTMFTPGGSHSEVEIPEILEHLRPQYPGVELRYAWPFDLGMIAGT
                     LAEQVRRFS"
     gene            10776..11351
                     /gene="pyrE"
                     /locus_tag="PK589_00085"
     CDS             10776..11351
                     /gene="pyrE"
                     /locus_tag="PK589_00085"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNJ18019.1"
                     /translation="MLQDELIAAFHKTQSFKWDPKGGFKLASGLLSPFYVDCRTLMAF
                     PHARHLVAKKAWEVIKDLQIDCLGGLEIGAISIATSISDYAYSAAPRREWRTFFVRKQ
                     AKDHGLGRLVEGVVRTGDRALIVDDVLTSGGSVVKTIVAAREAGLEVTDALVIVDRKE
                     QDGRARVEQTGVKVMSLLTIDDLMKGGPIPL"
     gene            complement(11352..11663)
                     /locus_tag="PK589_00090"
     CDS             complement(11352..11663)
                     /locus_tag="PK589_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18020.1"
                     /translation="MKRWSSCTVLTVSLFLSSCGEGALLVKDSADGGVVVYPYRAEQG
                     TLLSSFRKEAVRLMEQKCPGGYAIVREGETKGRLRQASPVAGSSDVVEERRWGIQFQC
                     K"
     gene            11729..12160
                     /locus_tag="PK589_00095"
     CDS             11729..12160
                     /locus_tag="PK589_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNJ18021.1"
                     /translation="MRYLTSIMCGLCLCGLLFLLDVPQPAVAYHSYVLSVQQLRAGLM
                     KASSTNAKGFVLVDVRSPEEHASGMIPGTDLNIDFREMKARHRELGAKLEDHIVVYCQ
                     SGHRSNIAAETLADLGYTHVYNVVGSMNAWTEAGFPVSPGR"
     gene            complement(12162..12917)
                     /locus_tag="PK589_00100"
     CDS             complement(12162..12917)
                     /locus_tag="PK589_00100"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNJ18022.1"
                     /translation="MEACPQVPSLLMLQIDPERYRFSIHQFRDEGLRSPISIHDWQQR
                     TDAYVLFNAGLFREDYSYLGVLLKEGRSLGTKKHHSWQGLFAAEPTDGTLKKARVLDL
                     AYEGFTEDPPLYREAAQSLMLFDRTGKLRVKDSGKRAFQTVVAEDGQGAILVIKTVDI
                     VSLHHLADCLHRQLPALQQAMAMDGGASSDVIASPDLLHAAQETSSQAGWRSLLAGTT
                     GVHIPLPTVIGITPRTPSRSTPAHGPSQSTRAH"
     gene            complement(13216..14859)
                     /locus_tag="PK589_00105"
     CDS             complement(13216..14859)
                     /locus_tag="PK589_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ18023.1"
                     /translation="MMTQPLRFIHLESNPADADLIDSTLREAGILCQPKRVHTREDFL
                     AALRQESFSLILADTTVPGFDGAAALGLARTLHPDVPFLFVSATQGEEFAIDMMQRGA
                     TDYIFKQRLGRLVPSIKRTLRELDERLERRRAEDALRMSEKQFRQAQKMEAVGRLAGG
                     LAHDFNNLLTVIMGHSQVLLGELPAGNPIRVKIEEMQKAGERAASLIRQLMAFSRKQP
                     VEPQVLPLNAILANVEGMLRRLIGEDIQLVIRPDPHHGHVKADPGQLEQVLMNLVVNA
                     RDAMPNGGLLAIETSQTELARTPMHHLHPLPLGHYVKLTVTDTGCGMDAEVLAHLFEP
                     FFTTKEEHKGTGLGLSTVFGIVTTCGGGIDVWSQVGHGTTFDLYFPRANPQTSAARSE
                     KPQSRPREGSETILLVEDDGGVRDLIRHELVKTGYQVIEAKNGVEACLTATQQSYHVD
                     LLLTDVVMPGMNGRELAQHLSVIKPNLRVLFMSGYLDDICVNSGMDPHRTTFLQKPFT
                     PDILLRTVRALLDSSSPATGQTQHPFSPPDQAPRTARAS"
     gene            complement(14877..15344)
                     /locus_tag="PK589_00110"
     CDS             complement(14877..15344)
                     /locus_tag="PK589_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ18024.1"
                     /translation="MIASKPILLAEDNPRDAELALAAMEEHHLADKVILCHDGAEVLD
                     YLYCRGHFKTRLQGNPAVVLLDLKMPKVDGLEVLRTIKNDAALRPIPVVMLTSSREER
                     DLAESYALGANAYVVKPVEFHQFLKAVKELGVFWGMINEPPPEGASRSAGRSS"
     gene            complement(15341..16837)
                     /locus_tag="PK589_00115"
     CDS             complement(15341..16837)
                     /locus_tag="PK589_00115"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNJ18025.1"
                     /translation="MNEPNRHQPVDVGLDSAFPPASPAEEKSSGRVSFLSPLLHTFQK
                     RSIEQRVLAGFGLVFAGILVISAISYRNMTVLIRNGDLDKRSHEFIQFLGATGEAIDD
                     AENGHRRFLVTGDESYLAAYNTLRDRAPEYIRYLRELTDPGSTQRGHVSDLEHLINQQ
                     LQEEHAAIELRAKTGFDSVRGMALTGVAKTALDSARRLQAQMDQEETKALTQRVVEST
                     TTTRSSIILLTIGALLLFVLLAAVYYLIRHDITERRRIADELHRRGELLEAANKELEA
                     FSYSVSHDLRAPLRHIDGYASLLAKAASISLDDKAKRYLQTISESATRMGQLIDDLLV
                     FSRMGRQEMLRGTVNLNQLIAALRHDLRHDLQDRAISWTIAQLPEVTGDAAMLRQVFM
                     NLMTNAIKFTGTRPQATIEIGSLDNGPDEAVLFVRDNGVGFDMRYANKLFGVFQRLHR
                     ADEFEGTGIGLANVRRIIHRHGGKTWAEGALGEGATFYVSLPRVRPLA"
     gene            complement(16996..18492)
                     /locus_tag="PK589_00120"
     CDS             complement(16996..18492)
                     /locus_tag="PK589_00120"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNJ18026.1"
                     /translation="MTFSLTLSASDLLLLLPELFLTLWLCVVLAVDFSFKRIVQEQLA
                     YLTILGVIITLACLAWFDMTGITGTLFGKMFVVDRMAIFFKVMILLATILVILLSIDY
                     VHRFSLFRGEYYFLVAMSALGMMFMASANDLLSLFVTLEFATFGFYVLVSYLRDDMAS
                     NEAGLKFFILGVFAAGLLAYGISLVYGETGKLVFSDMSGANPTTGLVIGFLLIFAALG
                     FKIGAVPFHSWIPDTYHGAPTPVTAFLSIAPKVAAFAILLRLFLVALATFKPAWALLI
                     VAASILSMTYGNIVAIAQRNIKRLLAYSGIAQVGNVLIGLAAGTKMGTDSILFYLLTY
                     LFANLGAFAVIMAISNSVGSDEIDDYRGLNRRSPFLAFAMLIFLLSLAGVPPLAGFIG
                     KLYIFVAAIKEGLYILITVGLVNIVVSMYYYLIVVKKMYINEPLDSSPIKTTGPLRAV
                     IYVGLAGTLVIGIYPQPFLDWAVAATLMFSNLIGPAASLTPPVTPFGG"
     gene            complement(18489..20144)
                     /locus_tag="PK589_00125"
     CDS             complement(18489..20144)
                     /locus_tag="PK589_00125"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNJ18027.1"
                     /translation="MAEYTLLIILFAPFLGALGLIFVSNRQVSLVRGIAAGSAFVSLA
                     ASVYLFYAYDSVKGGYQFIQRIEWSRQLGISLHLGVDGIGTPLVLASGILLFAGIFVS
                     WHIKDRMKEFYIWILILAAATIGVFMSLDLFFLYFFYEMSVIPMYLLLGMWGSHTKKY
                     LEMTDPEGLKQRDSVGFIFNFGANSKEYAAMKLVLFLSAFAVAALMGILLIYKFSGLN
                     TFDILILREKAHFSGPLATLIWLLIFFGFASIAPIWPLHSWSPVGHAAAPAATSMLHA
                     GVLMKLGHFSIIRVAFEILPETTRELMPIAAVLCIFSIIYGGLVAYYAKDTKYVIGYS
                     SSSHMGYVFLGMAALDYISLSGAVIYMFAHAMATGMLFAMAGWVYDQTHTRDIPSLGG
                     LSNRMPFISAAFVIGCMASIGMPGTVNFIAEVMIIVGSWNKYPFQVVVAVLGIVLTMA
                     YLFKMMRGLFYGSMAEKYSHSHDAVAVVDRMPLLIMITVSISFGIFPGHLYSVVRSGV
                     DPLIARITKVVPVAELPMNIPQASSHTMPVPVPGEAIAKVTSR"
     gene            complement(20148..21707)
                     /locus_tag="PK589_00130"
     CDS             complement(20148..21707)
                     /locus_tag="PK589_00130"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNJ18028.1"
                     /translation="MLEEFSFGFPILSYLIFLPLVGAAVLWLIDDEDLLKSTTLGITL
                     VELALACLVLVRFVPDSAAMQFAEHARWMPALGIGYHLAVDGISVLFVGLTAFLTVLV
                     VIYSWDTIRNQVRLYFMALLALETTTIGIFVSIDLILFFVFWELMLIPSYFLIKLWGG
                     GADRHYAALKYVLYTLLGSVFMLVGIALLDINYHQWAVSHHMDHVYSFDLLELLSVPI
                     PLSQQILIFWLMFMGFAFKAPVFPFHTWLPDALVEGPIGMAVMLAGMKLGTYGFLRFT
                     FPLVPDASKSEGVVGIVMVLALSAILYGAVVALVQSDFKRLLAFSSISHLGFVVVGLF
                     ALNFQGLQGSLLTMINLGFSTAGLFFMAGFLSTRQQSSHLSSFGGFAKQAPLLATFLL
                     LIGMASIGLPGTNGFVGEFLILLGAFKAKWWFGAVAVLGVIFGAAYFLWYYERAMLGP
                     LSKHVSAAVKDLHMREITIALSLSIMILWIGLYPSPFLRMMNGSIQALVDRLDRAKVA
                     SVDTVIHVPAK"
     gene            complement(21731..23734)
                     /gene="nuoL"
                     /locus_tag="PK589_00135"
     CDS             complement(21731..23734)
                     /gene="nuoL"
                     /locus_tag="PK589_00135"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNJ18029.1"
                     /translation="MSDSIDLIVKLIPLFPLMAVIVNGLFGHRYSHDLAHRFAWGSVL
                     MSFLCVLAVFTETLRTGAAREVIAYKWIFGGDLTINLAYLIDPLTCIMLLVITGVGFL
                     IHVYSVGYMHGEAGFTRFFVYMNLFMVSMLLLVMGNNYVVLFIGWEGVGLCSYLLIGY
                     YYDKVSAAKAATKAFVVNRIGDAGFLLAIFLVFVNFRTLDYTQVMQNAAQLSPEMATA
                     IALCLLVGAVGKSAQLPLYTWLPDAMEGPTPVSALIHAATMVTAGVYMIVRNHAIFDL
                     APMAMTTVAWIGGLTALFAATIGLVQTDIKRVLAYSTVSQLGYMFLGCGLGAYTAAVF
                     HLMTHAFFKALLFLSAGSVIHALSGEQDIRKMGALKSKIPWTHTLFLIGTIAIAGIPP
                     LAGFWSKDEIMGHAFVHHHYLLYGMAAVGAFLTSFYMFRLTYLTFYGTSRLDHHTAEH
                     VHESPMVMIGPLVVLATLSVIGGFPGVPPENGWFHHFLHSVAGVAGEEHATPPALMFG
                     LMGTATVIALLGWGLAHYFYSGPSTAAAALADRMPGAYTTLLNKYYVDELYDTLFVEP
                     TKQLGRLCDWFDRTIIDGLVRGIDRGTDLSSAAITWTEKHVVYAGLNIIGYANHLLAR
                     SWRRLQTGMVHQYAAIIVAGLFILVHLILLIWTGGGSTGYSAR"
     gene            complement(23736..24038)
                     /gene="nuoK"
                     /locus_tag="PK589_00140"
     CDS             complement(23736..24038)
                     /gene="nuoK"
                     /locus_tag="PK589_00140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNJ18030.1"
                     /translation="MIPLSAYVAVSAILFTTGLLGVLIRRNFIIVLMSVEIMLNAATI
                     NLVAFSHYLESMQGQIVALFIIAIAAGEAAIGLAIIIVVFRGKISTNVDEMNLLKW"
     gene            complement(24035..24559)
                     /locus_tag="PK589_00145"
     CDS             complement(24035..24559)
                     /locus_tag="PK589_00145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNJ18031.1"
                     /translation="MAFVFFAYFAVVSIVAGILTVALKNPVQCGLALLALLLHVSGLF
                     VMLNAEFLWAVQVIVYAGAILVLYLFVLMLLNLKTDDRYFHARTPYLLAPAVIGLIYL
                     LFLLIRSPFSGSRGDASTAAVLLNGDAHAVGIKMFSDYLLQFEIIGVFLTGAIVGAIV
                     LAKAPKSIETRRDA"
     gene            complement(24571..25158)
                     /gene="nuoI"
                     /locus_tag="PK589_00150"
     CDS             complement(24571..25158)
                     /gene="nuoI"
                     /locus_tag="PK589_00150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNJ18032.1"
                     /translation="MSVGALTKKVLQAALFYEIWDAMKVTFKHMFHRPITFQYPREQR
                     TIPDGHRGALGLLRYSDGRDRCVGCDLCEAACPSRCIKVISQEDPERPLQRFASEFYI
                     DITKCVFCGYCVEACPVNALAMTKMYEYSTHDKRTLLFDKKRLYEVGERHLEDAKKYL
                     YTHNQEKNSDESREYRYYFPQSVLKPTQSQPKHLS"
     gene            complement(25203..27896)
                     /locus_tag="PK589_00155"
     CDS             complement(25203..27896)
                     /locus_tag="PK589_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNJ18033.1"
                     /translation="MKPATNPDVEAATIELSIDGRTVSAKDGVSLYDVIASTGKIIPA
                     MCYHYTFDPFGSCGMCLVMQEGKKAPVRSCTAKAAAGMVIRTDGDDLFAARKKAVEKH
                     LSVHPLDCPVCDADGHCELQDMAFQHGVTNLANAKQKFIPEDTRSLVLDFNMNRCIAC
                     AECINVCKDVLMIDALQFMKKGGFNQVVAKGDQPLACEFCGDCLAVCPVGAITNKYSK
                     YLYKPWQMRKTTTTCNYCGDGCQMHLETKDTEVVRVTSPLSWKNKWGDRSETAKGHGG
                     LCVRGRFGFQYIDSQQRLRQPLVRTGDTLKETPWLDAMQTVIDQLSDIHRKHGPESIA
                     GLITARCTNEELYLFQKLMRGGLRTNQLDSSARYGHLNYVHAVRHAVGIGRPLNDWED
                     LTKAKAVLVIGSNITETNPLTAVRIKEAIRVYHAQVIVVDSSNTNIAQLASHPLLVKP
                     GTEALLIDGLVKAVIEQDLVDETSTTQHPQAFAALKQAVAQISLDEVSRTTGISVDQI
                     REVAAIFAEAPRAVALCAEGIVRRPNGYENVLKLVDLAWVTGKLGQPGCGVTTVTEEV
                     NEQGAIDMGVAPEFLPGQARFDDPGARERFGKAWEVTLPAIGTGANLLEIFTRIQKGQ
                     IKALYVIGENPLATLPASMDVKGALDKLQLLIVQDPFLTETGRMAHVVLPAATYAEKD
                     GTFTNLEGKVLRVRQALDQVGESLPDWHIMTALANGLGYEWPYQSPQDVQNEIMKLLP
                     GYYNLGQPRRIAPSADAYLSNGYSSEVVTRYRRRQAMDDTSRPFTLVMGQVLYHSGKM
                     STQASGLINIEPNNGRIRMNPADVEKLGLTEQSTVRLSSQQASVQAGVKADPDVQSGS
                     CFFPEHFNEPPIKDLMTTEVDAVTGVPYFKSTRVKIEKVGA"
     gene            complement(27982..29229)
                     /gene="nuoD"
                     /locus_tag="PK589_00160"
     CDS             complement(27982..29229)
                     /gene="nuoD"
                     /locus_tag="PK589_00160"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNJ18034.1"
                     /translation="MAQFEDQRTTVYKIDPEHPESETLPTLRTEELLLNMGPQHPSTH
                     GVLKVILELEGERLVKSTPVMGFLHRGVEKLAEEGTYHQFIPHTDRLDYVCAMYNNFA
                     YCRAVEKLMNITVPDRAEYLRTIVAEIQRIIGHQFWLGTQALDIGAMTVFFYCFRDRE
                     ILLDWFDELCGARLTTSWYRIGGVERDFTPSLFAKLKQFLDYFPPKIDEYVIFLEKNR
                     IWLARTKGVAVISAEDALSFGLSGPTLRGSGVDFDLRKYEPYSAYPKCEFSVPVGKNG
                     DTYDRYWIRVQELYESVKIIRQCLEQIQDGPIMADVPSVTLPPKERVFTNLESMIQQF
                     KLFSQGFNAPPGEIYCGTEAHKGELGFYIVSTGGGKPYRLKIRAPSFIHMGAFDHMSK
                     GYMISDAVTIFGTYDIVMGECDR"
     gene            complement(29258..29755)
                     /locus_tag="PK589_00165"
     CDS             complement(29258..29755)
                     /locus_tag="PK589_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNJ18035.1"
                     /translation="MSQLAQRIEDTFPGACTKVVEWRGDLAVTVKPETLHDVAKFLRD
                     DPALRFDYIVHVSSVDWPDDEDRFEVVYEVYSIRTRQRIRLKTRVPESDCIVDSLTDV
                     WKGADFMEREVYDMMGIRFRNHPDLRRILMPDEYDEGYPLRKDFPLRGKGWRDTFEFL
                     DEPTR"
     gene            complement(29772..30299)
                     /locus_tag="PK589_00170"
     CDS             complement(29772..30299)
                     /locus_tag="PK589_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNJ18036.1"
                     /translation="MGLIQLGGHDKDGSPDVITLTVEKAVNWARKGSLWPMTFGLACC
                     AIEMIAAVSSRYDMDRYGAGVFRASPRQSDLMIVAGTVCRRMAPVIRKIYDQMPEPKY
                     VIAMGSCATSGNIYDSYSVVQGVDRFVPVDIYVPGCPPTPEALFDGILKLQERIMQKR
                     VFLSQPKEVKDALKV"
     gene            complement(30290..30673)
                     /gene="ndhC"
                     /locus_tag="PK589_00175"
     CDS             complement(30290..30673)
                     /gene="ndhC"
                     /locus_tag="PK589_00175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNJ18037.1"
                     /translation="MAGSELLLEYLSRYFPVLVFVFIALAFGAGTLIISYFVQPKYPE
                     PEKLSTYECGSEPFSDARMPFPVRYYIFAMLFVIFDVEVIFLYPWAIVFNKIGLIGLI
                     EMLIFIGLFLVAYVYAWRKGALEWD"
     gene            31222..32781
                     /locus_tag="PK589_00180"
     CDS             31222..32781
                     /locus_tag="PK589_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ18038.1"
                     /translation="MRTLSMRASLTILSCSLMAVTAIPVYAQNAQSVRLGEAALTYAA
                     GSIRQDMPIEGVINVITGDNQLSGNRMMLGWSGTDTLYLKLKIPGDAALGDLYTVYRR
                     SRKVFHPITKQYLGYVINRVGVVKVVQLDPVLVGVQVVRSYAPLSPGDPVMRFTPPAA
                     EEVSEPVSSGPDVEGMIVELQADKHMSLVSQGNVVYLDKGRDDGLRSGDTMEVFRTGG
                     GLPERKIGEVRVLATEPHTATAVLSKASARALVGDRLRSKHGVPVEAMQPESGEIDRQ
                     AATVQPVMVAKTDASVREANGGRLPATGKIERTRGSRRLNLDDLADQLEYESGEVKVK
                     PAGVPILEKLTEYLTTTATGQQVRVEGHSDNMEIGPSLKGVFPTNWELSKARAAEIVR
                     YLVEKGGMDSAKLSAVGYGASRPVASNGTEEGRKRNRRIEVVLESLEEAAPAQPVKAS
                     VDETASVSTQFTYNQMNAVPVNPTSVPVSEVPSGPAPSDVTPERGPIAPEAGVPASPA
                     DGMPPSPAPGS"
     gene            32943..35258
                     /gene="priA"
                     /locus_tag="PK589_00185"
     CDS             32943..35258
                     /gene="priA"
                     /locus_tag="PK589_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNJ18039.1"
                     /translation="MLDPPLQQPSAMIPAPSPAYVDVVLPRRLHRPFTYIIPSELKGR
                     VAIGRSVVVPFGSQDLHGLVIAVHHRLPPGAPQKGLKAIRSLSAASADHLLTAGQIGL
                     SHWVAERYAAPWGQCIKLVLPPVDRVGASQPRYMATAQGLATNASVEPLGETETELLA
                     RLRRRPKGIMATTLLKGDRSRTTLALQALIQKGLVSRCEEPAAQKSVRPAKQQGQVSG
                     GGLPMESVEAVENFELPHAEAMSWPTVLEQALAKKVFVSLLLEGSHCTRQWCLTQAVR
                     ATLRLGRQALILTGDVEHASRLADSFAAEGEQPMLLHSGLSMTARAAVWQAAQEGSVR
                     ILIGTRMAVFAPLRRLGLVWVDGEDDASFKEEQVPRYHAREVAHYRASRDGAVLVLAS
                     NHPSLESLSAVQEGRMTACKYRDAGELPAVHVIDMKEYPRDASGGTVLSPPLTEGLRE
                     ALRQHALSILYLNRKGFASVLHCGDCGAMPQCDACSVALTFFRRSNHVRCHYCGRTKP
                     VPEHCSRCQSLKLEPVGSGTERVEEAVRRLFPQARVGRVDGETIRRPADARALNRLLA
                     AGELDVVIGTQMIFRLGLEGRALCVALPDADAGLHIPDFRSAERMYHALRDAAELARP
                     ASEGGRLLIQTRLADHHAIMALSCGDEEVFVRQEQAFRQMLQYPPWTRLVRLDVSGTQ
                     EAMVSQAADRWAALLKAQAAGREPMVVVLGPSPAPHVLRRGRYCWQILIKSASLEAGK
                     EIAVRTREDVERESRRGGLRYDIDVDPVSMA"
     gene            complement(35265..35699)
                     /locus_tag="PK589_00190"
     CDS             complement(35265..35699)
                     /locus_tag="PK589_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18040.1"
                     /translation="MTWWTPQLNALLGGLVLAGGIFVMWDQAGLVWTLLVAVAATAFL
                     LWRGRSIGTVWAWTTLGLGIESMTWPIVTMVRIRVEGIQPTEEQMGTILNAVLFGLFT
                     SVFWVTFALGLFKRLRAQHAPPVPVQQGQQATPRGKKKRSRN"
     gene            complement(35812..36183)
                     /locus_tag="PK589_00195"
     CDS             complement(35812..36183)
                     /locus_tag="PK589_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ18041.1"
                     /translation="MPSVLIVDDEEAIRRLIRSTLEQAGYRVQEAADGKEGLSHYRQA
                     PADLVIMDILMPDQDGLESILTLRREFPNAKIMAITGGSDMIGILNFLDVARMLGARR
                     TLQKPFEMQQLLDAVQAEIAG"
     gene            complement(36290..37900)
                     /locus_tag="PK589_00200"
     CDS             complement(36290..37900)
                     /locus_tag="PK589_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ18042.1"
                     /translation="MSAQDLAGAFPSSGDFRSIEAVLRTLSSQASIGIFLSDPEGHTL
                     YLNERLRRMAGLPTAPASPECWLTALAPEDHDVIVTEWLTATEQARSFSREFRFCRPD
                     GSLRWVMAEAFPLRTEGGPPTGYVGMIRDITPRHLALEALHAAEDRYRSLAALSPHPV
                     LVYADGTILFMNDAGMRLFSVPPAASVEGHSLSDVFPKAFLQDLPVANPIHGTEGATP
                     VERQFVRPDGTRLDLEVVASQVTVDGHPAIQVLATDITAQKDAAAQVRRAHKMAAVAT
                     LAGGIAHEFNNCLTAIMGFSDLALPLLVPDSRVYGHVQQVVLASKRARDLVTQMLMFG
                     RQAEGMKQPVSLDILLKETLRLLKGTLPDNITLREWIPGATHPIYADPTQIHQVCVKL
                     LAHSELAMKPDGGILEVRLDNVDLTQSTNAEDLPLRPGHYVCLTVSDTGEEVSAQDHR
                     HKVGPLFAHLPDGTQAGTELAGVQRVVSEHGGTLHSTSTVGQGTTIEVYLPAILPPRA
                     IGAPELPRADASDITGQKEFLAERDKER"
     gene            complement(38304..38387)
                     /locus_tag="PK589_00205"
     tRNA            complement(38304..38387)
                     /locus_tag="PK589_00205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38352..38354),aa:Leu,seq:gag)
     gene            38615..39310
                     /locus_tag="PK589_00210"
     CDS             38615..39310
                     /locus_tag="PK589_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HNJ18043.1"
                     /translation="MTPLSAPHISCTGRQTDIAALFDVDNTLLPGQASEVRFFRFLWK
                     RGLVGWREVRDSIHWVLRCAPPVSLHPLRERKLYLAGKGAAEVEALAEEFCRTELCPR
                     LSAQGLSRMDEHRRAGHHIVLVTGSLDFLIAPLAALLEVPTLLAARLEQQEQQFTGHV
                     CAPLPYGPGKRELIARLTRESGIDLAQSFAYGDSPGDVELLEMVGHPLVVNPIRGMDR
                     IAQRNGWPMTTWK"
     gene            39361..39999
                     /gene="queE"
                     /locus_tag="PK589_00215"
     CDS             39361..39999
                     /gene="queE"
                     /locus_tag="PK589_00215"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNJ18044.1"
                     /translation="MLRVTEIFHSIQGESTHAGRRCVFIRLTGCPLRCTWCDTAYAFY
                     GGQDLTEEDVVAQVRAFGCNLVEVTGGEPLSQAACLPLLRRLCDEGFEVLLETSGAVD
                     TAGVDERVHVVLDVKCPGSGMVDRMYWPNVDRLTRRDEVKFVIKDRTDYEWARKVVRS
                     RDLEARCTVLMSPVFGEVDARQLAEWVLADRLPVRFQLQMHKQIWAPDMRGV"
     gene            40064..41584
                     /locus_tag="PK589_00220"
     CDS             40064..41584
                     /locus_tag="PK589_00220"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNJ18045.1"
                     /translation="MKKIQVQAQVGRAEQEGAEVLVLLCCEGASDLSPEAAAINTQLG
                     GQLATLIQRGEFEGKLGEGLLVHTQGKAKAKRLLLAGLGKEKDLRLDAFRQALGAAVK
                     RVRQAKVSSFGVVLPGAVLEEIPVQDVAQAMAEGAILGNYQFTAYRSSNGSKPVEVER
                     LTIYVAQTSLLAQVTEGIRRGVATAEATVLVRDLCNHPANIMTPGRIVQEAKAVAKEP
                     GVKLKVLEQKDMEQLGMGALLGVAKGSHEPPKFIILEYKGAKAKKGDPPVVMVGKTIT
                     FDTGGISLKPAENMEHMKADMTGGAEVLATMRAAARLKLPVHLVSILPVAENMPGGRA
                     MRPGDIVKTLSGKTVEVQNTDAEGRLILSDALAYATRYKPAVLIDIATLTGACVVALG
                     QFAIGMFGNNDQLKEHVRNAGMRAGERVWEMPLWEEYFEQLRSDVADMRNIGGRGGGM
                     ITAALFLSKFVGDCPWIHLDIASTDWSERERAYLPKGPTGIGTRLLIQFLLDRTLP"
     gene            41659..42810
                     /gene="nagZ"
                     /locus_tag="PK589_00225"
     CDS             41659..42810
                     /gene="nagZ"
                     /locus_tag="PK589_00225"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNJ18046.1"
                     /translation="MTLREHIGQLFMMGFTGTTVSKDLASFLTAYKPGGVIFFRRNLE
                     SVQQIVDLTNGLQKLSPASPLLIAIDQEGGRVSRLPAEFTIFPPCGQLGQCHSSELAY
                     SAAATIAKELRAVGINMNMAPVLDVNSNPDNPVIGDRAFGAEPDVVADMGLATIGGLQ
                     DNMVVACGKHFPGHGDTATDSHKELPVVDAGIQRLRETEFPPFQQAIRQGVASLMTAH
                     VLYRALDPDAPATLSPAVIQRLLREEFRYDGVVFTDDLEMHAIIDHDGIGEAAVRSFV
                     AGCDVLLICKDQDRVMTAMQAMERAVQNGRITQERLEQSLARVARLKARYLIPYKPVT
                     ISDARLVVGCRSHKVLLDSWHKAYARLPSSKVSEHDEPAAALDSPVTHV"
     gene            42961..43350
                     /locus_tag="PK589_00230"
     CDS             42961..43350
                     /locus_tag="PK589_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18047.1"
                     /translation="MGIKKGDILDERKRFPDSCGLVVKCTGGGEGFQKIFCCGHELTE
                     EDLVSEIVSSRGRKRGTLLPGAMLEERRQFPDSCGLRLMVMDGGAGLQGIDCCGHLIT
                     AGAVHGLRFGQPLDEGAPMPTGPAGQA"
     gene            43350..43871
                     /locus_tag="PK589_00235"
     CDS             43350..43871
                     /locus_tag="PK589_00235"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNJ18048.1"
                     /translation="MRAPSMPGADEKFHRPSMRWWLGFMDQLDRLGYVAAGFSLLALC
                     LIIFVHAWYIFLARPVQLALLPAALKLLNDLLLVIILLELFRTVVRFLQTEVLELEPY
                     LSVGIIACTRRLLTASAELSYQLEAVAKESRHELFQQYLLDVGLNVLVIIILIGGLYL
                     LRSRPSTERPAVS"
     gene            complement(44284..44451)
                     /locus_tag="PK589_00240"
     CDS             complement(44284..44451)
                     /locus_tag="PK589_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18049.1"
                     /translation="MTPLHEHFDDGETKTTLRLARLGRPWVRLRQGPQQGRPVWQQPL
                     PVQLGQVRQLK"
     gene            complement(44448..45893)
                     /locus_tag="PK589_00245"
     CDS             complement(44448..45893)
                     /locus_tag="PK589_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNJ18050.1"
                     /translation="MGNPLFRTKSIERILADSDAPEHRLKRSLTAWDLTGLGIGAIIG
                     TGIFVLIGTAIVGDAHRPGAGPGIVLSFILSGITCALAALCYAEFAAMIPVAGSAYTY
                     SYATLGEFLAWLTGWNLILEYGVACVAVAIGWSGYFNNILKLCGLELPYWATHPPGAD
                     GGIANIPAAIIVLLVTGILIVGVKESARATCGIVLIKLAVIVFFIAVGTSSVDTTNWT
                     PFMPFGFAGVGAAAAIVFFAYIGFDAVSTTAEEAKNPQRDLPIGIFASLGICTLLYIA
                     VAAVLTGLVPFSRIDIHAPVAEGLRVAGFKWGAAIVAVGAVAGITSVLVVMMLGQIRV
                     FFAMSRDGLLGPWLSGVHPKFRTPHHATYLTGIAVAIMAALIPIGEAADMTNIGTLFA
                     FVLVCIGIIVLRYTNPNHPRPFRMPFMPVIPMLGVLACLGLMYFLPWMTWIRFFVWTV
                     IGIVVYATYGIRRSKLATSPALEHTSHARRA"
     gene            complement(45957..46733)
                     /locus_tag="PK589_00250"
     CDS             complement(45957..46733)
                     /locus_tag="PK589_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNJ18051.1"
                     /translation="MKPVIGVTPDFNAGDRQEWGGKEPTYFLRARYVRAIEELGGVPL
                     ILPLVADRAARRRLLRTIDGLLLTGSGPDLDPALYGERKQYSFPIVAQRRSSFELDLV
                     HLATRNHLPTLAICGGMQTMNVAFGGTLYQDLPAQVPDVLQHRQTTPAIQLAHSVSIT
                     PGTLLERIMKRPRMKVNSSHHQSVKVVGRALIASATAPDGIVEAIEHPGHPFFLGLQW
                     HPEFLFERHRLHRQLFEAFLRAASRRPALRPLSPQRGTTT"
     gene            complement(46735..47211)
                     /locus_tag="PK589_00255"
     CDS             complement(46735..47211)
                     /locus_tag="PK589_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18052.1"
                     /translation="MNVSVNLSSKRRRSLWSNALVLGLVAICSLAILIVPVAAQESGS
                     ASNVPVSSNDASPSGVGIQAGSALATIIYFPLKLAFAIGGGVVGGLAYGFSGGSEQTA
                     KNIWIPSMYGTYIITPDHLKGDKPIRFLGVSSSEPTREAGDYTPQEPLPLPLEPIR"
     gene            complement(47368..47829)
                     /locus_tag="PK589_00260"
     CDS             complement(47368..47829)
                     /locus_tag="PK589_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18053.1"
                     /translation="MSIPPAATEPSQYRVTFFFGPEPVEHRPDLLRCVFNVKKRSWKG
                     GIQVSLDIAMKHVEEARRAIGFSTWVEHQLHKICQEEQAELTRRADELFIQSVCTCAL
                     NLALHGGIEQHNQVIDAARFTDELRQIMTEQPEQVMERIRLELDLEESPDR"
     gene            47921..49138
                     /locus_tag="PK589_00265"
     CDS             47921..49138
                     /locus_tag="PK589_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNJ18054.1"
                     /translation="MKTSADFVAQVDRIQEAIRAHPGIDGWLFYDFRHLDPIAYRVLL
                     LDPSMHVTRRWYYWVPAVGMPVKLQHRIEPHVLEALPGEVRFYVSWREQQTALRALLQ
                     SAKRIAMQYSPMNAIPYLSRVDAGTLDLLRSWGLEIVTSADLVQQFEAVWDEAQLASH
                     QAAATGLRAIVDEAFGFIGTSLAAGSSLNEYGLQQYILSRMHARGLVTSSPPIAAVNA
                     HSADPHYGPSAEGSAPVRQGDLVLIDLWAKQPEARAVYADITWTGFVGKVVPARQQDV
                     FQIVRQARDAAVTFVQARVREGTFPYGWEVDDVCRQVIQQAGYGEHFVHRTGHSIGEE
                     VHGNGANIDNLETQDARRLLPGTCFSIEPGIYLPGDFGIRSELDVYLSTNDAVVFGQP
                     VQTELVAITLPTT"
     gene            49273..50127
                     /gene="tatA"
                     /locus_tag="PK589_00270"
     CDS             49273..50127
                     /gene="tatA"
                     /locus_tag="PK589_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNJ18055.1"
                     /translation="MFGTMGFSELIIILVIVLIIFGAGKLPQIGEGVGKALRGFKKEV
                     NDIPAADATPEQGEAATPSSQAQIPPVAEVAQTAQPAPVAQAAPSLKPTAPYTPGPEL
                     TPGTTAALMAAAAPQGPQTAQASKPRVATVTPTQAAPGSVVAQGHQPPTMEDRMAAPA
                     PVMRGQYPPLPASAQSKPVAKRPSAIVNKDAVARVQAAQAAIRAKAAQPQPAAFSPQD
                     MQGLGEGLGDAVRTFRQAVADVRSSVDPQMRTIRAEMDSAQKELEQSIEAAKQAPVLD
                     EDTPAKPL"
     gene            50442..51128
                     /locus_tag="PK589_00275"
     CDS             50442..51128
                     /locus_tag="PK589_00275"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ18056.1"
                     /translation="MRLNELLADPDPEVRRTAAEGLGKIGHPSASPGLIAALADRDPH
                     VRAAAALALGRVGDAKSGAALVEHLSDSSESVRTASALALGEIGTSVNHEEKILRALR
                     QPEVSTRLAASRALLSLETVSFSTDLLSALDDPNAQVRQGVVAALSETGDVRSLPGLV
                     KLLQNDADAGVRAEVAFRLGKVGDAGVVPALSVVAGKDSNPTVRQWAHWAVRQITTSP
                     GFGSGNRQGQ"
     gene            complement(51082..51600)
                     /locus_tag="PK589_00280"
     CDS             complement(51082..51600)
                     /locus_tag="PK589_00280"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ18057.1"
                     /translation="MKFPVTATREHHGKTLGIDCEQETVSLFDHKGQQLSILSWGTLI
                     EHMLSVDEDARFAHSRSHPRAPLAIKVHCTTEDGKHFDSLTGGIGGGGLFIESSTPLA
                     PGTELTVEFALPDRPNQKFATRAKVAWTRSKPERYLLFPGMGIQFTDIDPVAQTRLID
                     LVDSLNRSRAMS"
     gene            complement(51677..52195)
                     /locus_tag="PK589_00285"
     CDS             complement(51677..52195)
                     /locus_tag="PK589_00285"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ18058.1"
                     /translation="MKFPVVSSTRHKGKTIEIDSSKESITLLGINGEPMGSLAWDFLV
                     DQILTYRKPQQSREARSEPRISLSIRVKYHTPDGMQFESRAGGIGGGGLFIESLAPLA
                     VGTKLAMEFTLPEDPSEWMSAKGVIAWVCPKADQYTFSPGMGVRFTDIASETRSRVLN
                     LVHSVKGIPQSE"
     gene            complement(52406..53662)
                     /gene="clpX"
                     /locus_tag="PK589_00290"
     CDS             complement(52406..53662)
                     /gene="clpX"
                     /locus_tag="PK589_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNJ18059.1"
                     /translation="MAKQEKIDRHLRCSFCGKSRDEVRKLIAGPTVYICDECVNLCND
                     IIAEDWEEAKEEISSKLKKPAEIKHHLDQYVVGQDRAKRILSVAVHNHYKRISAKDKD
                     VDDVELQKGNILVVGPTGTGKTLLAQTLAKFLDVPFTLADATTLTEAGYVGEDVENII
                     LKLLQAADYDVERAERGIVYIDEIDKISRKSDSPSITRDVSGEGVQQALLKLIEGTVA
                     NVPPQGGRKHPHQEFIQVNTSNILFICGGAFVGLEHIIEQRLNRKSMGFGAEVRGRND
                     IRLGELLPHVQPEDLLKYGLIPEFIGRLPVVATLDELDEQALIRILTEPRNALTKQYE
                     KLLSFEKVKLRFTEGALSAVARKAFTQKTGARGLRAILEEAMLDVMYDAPSQKQIKEV
                     VITEDAISGKHAPIRIFEQDKDVKSA"
     gene            complement(53689..54324)
                     /gene="clpP"
                     /locus_tag="PK589_00295"
     CDS             complement(53689..54324)
                     /gene="clpP"
                     /locus_tag="PK589_00295"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNJ18060.1"
                     /translation="MLVPIVVEQTNRGERAYDIYSRLLKDRIIFLGAPIDDVFANLII
                     AQLLFLEAEDPEKDINLYINSPGGSVTAGLGIYDTMQYVKPSINTICLGQAASMGAFL
                     LTAGTKGKRYALPNARVMIHQPMGGFQGQATEIDIHAREILKIRERLNEIMAKHTGQP
                     LDKISQDTERDYFMSSEEAKRYGLIDEVITRPLKSLKPVGSTDSGKDGGKS"
     gene            complement(54449..55774)
                     /gene="tig"
                     /locus_tag="PK589_00300"
     CDS             complement(54449..55774)
                     /gene="tig"
                     /locus_tag="PK589_00300"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNJ18061.1"
                     /translation="MKMEMTELGPMKRALKIEVPADEVNLRFVQAYSELNRQVRIPGF
                     RPGKAPLQLLEKRYAKTIEEDVIRSLVPDYYDRAIRQAGIVPVLVEIPPLERVKVKKD
                     SPFSFTATVEIKPTIELRDYKAPNPISLKQDQRTVTDEQVQKAFEVLREQMAQLHPAP
                     AGTTLADGDYAVVDIDGTVDGAALDGTTKTGHLHKMGSQSSVLGIEIEPHLLGKKEGD
                     VITIPQAYPATHPDARVAGKTVNFRCTVKSIKRKQLPALDDEFAKDCGPYQSLQEIRE
                     KLHAEMDRALKKDIEDSYKDTILKRLAETHHFDLPGTLVERELSAMVRQQLQSRQRKT
                     GDAADGSPGASQADEAKQLQEEYRPEAERRVKVGLILEALATKENITVTNEDLSNEIA
                     RLAAEVKLSVEEVTRMIQAGGQDTLNDLRSRILADKALDFVYKHAMIQG"
     gene            complement(55835..55917)
                     /locus_tag="PK589_00305"
     tRNA            complement(55835..55917)
                     /locus_tag="PK589_00305"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55881..55883),aa:Leu,seq:tag)
     gene            56250..57335
                     /locus_tag="PK589_00310"
     CDS             56250..57335
                     /locus_tag="PK589_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ18062.1"
                     /translation="MKRQSVTAALGCLILLCGFPLSSEAYRDYFTDAQKQELVKIQTV
                     LIEALALTDKGAGNADGILDVAMKRMGELGYTTVSDPHVSHDVVVRVKCEQKKTWEGT
                     ASAGGDNDLPDAPSRLWKGPACQITYALGGMKVKWQKEVRTEFEDAVGAAQSAQAPDP
                     GLYAMNALHDALETYEFPLLLTAEWGQPDRLLKLLDSPQTSQIRKLKIISLLGEMVAD
                     EALPHLTEALKDKDLAKQAAVALGNMGKEGIPVLIDILKHSKQPELQAAAAKGLGDLG
                     NIHSDSRVVPPLLEMLDAPGIDIVVQTEVAWALGRVPDRRSVEPLFALDRKLQKIRKD
                     PPDPQIKKLKEAVFWSIKQVYTEDQYS"
     gene            57609..58850
                     /locus_tag="PK589_00315"
     CDS             57609..58850
                     /locus_tag="PK589_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ18063.1"
                     /translation="MRDISRRRFLQISAMSGGVLLLGDLAERILNVPLLRSAALAAEP
                     IKIGILDPLSSPYKTSSIHDVHGANVAVDLFNKRGGVLGRPVTIIEADDASSPDAALK
                     AATKFVQDDRVDVLMGTFNAECALVVSEYAKKANKLFMVTGAHLPELTGAACNSHTFV
                     FMPNASMLAQAVVPQLVKAYGTRWYMITTSSLDGKAMAQAMASTGLTHGVEFVGETLM
                     PFGSTDFTSALSAAKDKRPTLVVLNLYGWDLVHALKAYTKLELAKDKIGVGGMIAGEQ
                     IGRPLGYANNAGIWGLIWDPKVNTDGSKRFIQGVVEKYNHTPTSRCYLGYAAMTQILE
                     AIQRAGTTEASALIKVLEGHEFDGLKEGRSYFRASDHQHVQDVLVGEAYGKELGLGHY
                     KILATVAGESVATVQSGSCQL"
     gene            complement(58872..60323)
                     /locus_tag="PK589_00320"
     CDS             complement(58872..60323)
                     /locus_tag="PK589_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ18064.1"
                     /translation="MRRAVWALMVLGALASACETVPQPQPVAAPPQVGDAKLLALQQE
                     REQLLTTLGEFHDRIRDLESKLGDRQNQPAAASYDQLLNAKDAELAELRRLSPERDKL
                     TSQLAMANNELLQARQRIGSLEQQLATREKDLVALQGRSTAISDLEVARRRIMDLEAQ
                     VARQDHDLRTIKSENAERDSLAAQLQTATATIDSLKARISALDQQLRDREQAFETVRS
                     RLMERDKLVPQYNAMIAEIYQARHRIAALEQRLNEKSRDLSPRQKGAQAATAPRETGA
                     TSNKNAGSSEKDAGRTGSATPTSPSQSTSREASFAAMKEEILKVLPSDGGQKAITVKQ
                     DGSRLTIALSSNWLFASGEAALTSEGMTMLKRIGTVLGPLSDKFVQVAGHTDNQALSK
                     TLQKTFSDNKALSWARAENARRALVTGGMSPERTKAVGLADSRPIASNATEQGRQKNR
                     RLELVIVQGPTVASTTGKTTEERDRLAALAPSY"
     gene            complement(60570..61772)
                     /locus_tag="PK589_00325"
     CDS             complement(60570..61772)
                     /locus_tag="PK589_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ18065.1"
                     /translation="MKHVCVVPVLLSAALLAACTSTPAPNDSASRALNEMKAVARPIP
                     PKPDPRDLRIAELERQKTDLEGELDRLRSSSAADSDRSKARITELENQLNQRDRELAG
                     LRNAAGDKDRLAGQLSDADRRLSAKDQELAALKQSAADKERLAAELAALQGLLATKDQ
                     ELAGLKHNAGDRDRLSSELAQAKQHIADLERQLSGKDQELGTLKGAAGDRDRLVADLA
                     AAKQRASDLENEIGRRDHEMAGLRGALDQQKTSLAEAKNDLSKLLQAEVSKGNVTMKQ
                     LGDQLTLGLATTLLFDSGEATLKPGGADVLNRIGSVLKNYPDRSIHVAGHTDNVPIKG
                     RLAKRFPTNAELSQARAESARQALTEGGMAAEKISAKGHADSRPIASNSTAEGRQKNR
                     RVEIVVGQ"
     gene            complement(61841..62266)
                     /gene="mscL"
                     /locus_tag="PK589_00330"
     CDS             complement(61841..62266)
                     /gene="mscL"
                     /locus_tag="PK589_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNJ18066.1"
                     /translation="MGMMSEFKEFAVKGNVLDMAVGVIIGGAFGKIVSSVVSDILMPP
                     IGLLMGHMDFSSLFIPLSEEAKGKSLAAAKAAGAATINYGVFLQTLLDFTILAFVIFL
                     VVKQMNRFKKATPPGPPPAPPKEEVLLTEIRDLLKNQRH"
     gene            complement(62317..63429)
                     /locus_tag="PK589_00335"
     CDS             complement(62317..63429)
                     /locus_tag="PK589_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNJ18067.1"
                     /translation="MMRRYACFFLALLIVALTAYGVNAESPTPTDTTAAAPTLDSVTL
                     KDGTVIYGRVLGMIADELHIKTGFGPTAGDDIVKIMWPNVAKLVVNRPIPFTLKEGTT
                     VVGTAQPGEPGTLTLQAAPMGTPMAIPLDAVVGMNQPAVIYTGALQAGFSQTTGNSHL
                     RNGSLLGELSARSESLRLTILGRYIYGDNSGNLIVRNSRGTIKLDFFLTKRLYWFASA
                     YFEQDTFQDLKLRTALATGPGYQLLDRGDLTGFFKDMTLWAEAGAAYFNEDFKLADDK
                     SSTRGRWAVKWNWPLWGGDQVSLYHFQEGFQSLANSKDLYLTADTGLRFKVWGGLVSG
                     FQWTMRYNKNPPPGVSDTDNLYLITLGYSFDTSRKQ"
     gene            complement(63816..64127)
                     /locus_tag="PK589_00340"
     CDS             complement(63816..64127)
                     /locus_tag="PK589_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ18068.1"
                     /translation="MDKVFPFSTNRPDQPTPREQEILNYIWAGLTSQEIAARLQIAQK
                     TVESHRANLLRKFRATNAAQLLRLALLEGLLSVPSTERAPDVKRVVQSAKASRRLTSI
                     K"
     gene            complement(64390..66180)
                     /locus_tag="PK589_00345"
     CDS             complement(64390..66180)
                     /locus_tag="PK589_00345"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ18069.1"
                     /translation="MAPDPSLRTPDFLCSLFERLEYVVLEHVAGTQFRILGHPSPWLF
                     RLYPEAEASLQLSVDSRTPVLHNFLADAAEVWQTGDAHIGRSGFWTEQTAFDVPWHFE
                     ALALREGTSRLLVIQHSTAAYEQQAAMLQRAREQALQQHEQERGHQRTQQDLTMQLVD
                     MERARDDVAAILQDLGLATLLIDQEGHVRFVSASAASLLERPSASIKPDLRWETLLPV
                     TKADRLTVQSLLEHPIPQRERVCCHIDTPAGRRLWLDLELHDDPRDRRSKIVFLHDMT
                     DVYHLRRMLELKAHYHDLIGKSRGMTQIYEQLQDLARVDSTVLIEGETGTGKELVARA
                     LHQASARRTGPFIAANCAGLTDSLLGSQLFGHKKGAFTGAIDDQQGLFEAAQGGVLFL
                     DEIGDIPHTVQTNLLRVLQEKEVTRLGETKPRKINVRVLTATHHNLSQDVAKGTFRAD
                     LLYRIRVARIHLPPLRERKEDIPLLVTSFLAEGRASMGKLIHRASPATMAALMDYQWP
                     GNVRELKSTIECAMIHCKGDSLEATDLPPELQREPTALTPEPITTDERSRFVSALGQA
                     RGNRTKAARLLGMSRATFYRRMTELDLPAN"
     gene            complement(66170..66706)
                     /locus_tag="PK589_00350"
     CDS             complement(66170..66706)
                     /locus_tag="PK589_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HNJ18070.1"
                     /translation="MIEKKICMLGAFAVGKTSLVRRFVSSCFSDQYQTTIGVTVDKKS
                     MTVDGTSLTMVLWDLYGEDEFQKLRSSYLRGSSGYLLVLDGMRRATLDIALHVQERAS
                     DTLGPVPFVVLINKHDRRAEWDITDQDLAQLAQRGWSVVMSSAKTGQGVEEAFTTLAR
                     AMLTPSNSSAAAGRPHGS"
     gene            complement(66717..68447)
                     /locus_tag="PK589_00355"
     CDS             complement(66717..68447)
                     /locus_tag="PK589_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18071.1"
                     /translation="MAQPPNRSTPPAEHDYAELRSLLLAPEQTRLEQLQEQVEHIDLN
                     AKNLSRVLPDAIALRGEGDHQLTHVLTPHVSEALGASVRRQPHMIVDAIAPIMMPAIR
                     QAIANALRGMVQSLNQTIEHSLSIQSVKWRVEALRTGKPFAEIVLLHTLRYRVEQVFL
                     IHSQTGLLLAHAAGDAVAVQDQTLVSGMLSAIRSFVQDSFGADPEQVLNTLQVGELTV
                     WIEQGPAAILAAVIRGTPPESLRLHLQDTLTRIHAERADALARFTGDAATFASVTPLL
                     EECLRAQFESRRRGIAPMTWVLIIGALLVTTWWALSTYQERQRWQSYLSRLATEPGLV
                     VTDAEPEGGRYRLTGLRDPLAADPAILMQDSGIDTSRVVAKWQPYYALDTSFILKRAG
                     FALSPPPTVHLALEGSHLTAGGTASADWIRQSRSVARAIPGLDGYDDRRLVSQSLDML
                     RQQFTETSILFEQGSSNLVSPEQLQQARRIVGMLRQLEDLARLASAAVTVSIVGQTDV
                     VGGTSQNRRLSDGRAKAVLDAIHPASFPALTFHTRGMAQEAELQRSTPAVAHPQHRRA
                     SLEITIQPMP"
     gene            complement(68564..69178)
                     /locus_tag="PK589_00360"
     CDS             complement(68564..69178)
                     /locus_tag="PK589_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18072.1"
                     /translation="MSRQTDLDRTRRSERTPTTLAETETETALADDPTAGNNLDKIRD
                     ILFGAQVRDHERRFTKLETQLLAEAAQLRNDLKDRFASLEQYIRTEVEQVTGRLNAEE
                     QGRTKAVSALTAELHTLAGVLQQTATQLREQSEQADRDLRDQLQHQAASLTGEFTQQH
                     ATLSATLDQAVQQLSHQKTDRTSLATLFQELSQRLSHDQPPQQA"
     gene            complement(69317..70117)
                     /locus_tag="PK589_00365"
     CDS             complement(69317..70117)
                     /locus_tag="PK589_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNJ18073.1"
                     /translation="MSLANLITQYVVQYGFRIMGAILVIVAALFVANSVGRLFERWLN
                     EQDLEPPLRLLLLRLLKALLVILGVLIALDQLGVQIAPLVAGLGVAGLGVGLALQGVL
                     SNVVAGLSIIFTKPYRVGEHISLLGVHGDVAKIDIFTTTLLHPDHSRIVIPNRKVVGE
                     ILHNFGTIRQLDLSIGVSYQTDIETTLRVLRELVSRHPKVLQDPVPMIGIAAFADSSI
                     TLSIRPWVPVASVGSAHIELNQAIIQRLRADGVDIPFPQREVRLLNPS"
     gene            complement(70135..72243)
                     /locus_tag="PK589_00370"
     CDS             complement(70135..72243)
                     /locus_tag="PK589_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNJ18074.1"
                     /translation="MKILVGIGVVILLFLILIIALPFLIDLNKYQDRYRPLIEEALNR
                     KVELQDIRLTIWPRIGARIGGFVVQDDPAFRTGPFASLASLDVGVKLLPLLKGRVDVE
                     EITLRDPVILVLKNSQGQLNISTIGAKPTSPAAPSKPEAPGQPAGSPLQALALFAVDR
                     VSIDGGKLTYRDESTAKPTEYTVTQLEFLLTSVHLGDSPTVHLGATVLPYNLPVHLDG
                     TFGPLVETLDVKSFSFNLGLGKVAVGLKGRAVGGNLDATINALQIDTADLPIALPLTK
                     PVQIKDLHLTLHALYPIPPDTPPASQVDLTDLGLTLVMGGSAINIKGTATKGLANITA
                     ASASINSSDLPVAVPLAKPVEVKDLHMNVRAKYPPKEGTAPLELAEVPNLGLTIALGS
                     SRVEVKGSVLGGLAKVTATSKLVNSADLPVALPLKKPVEIKDLQAAAEMKGQEARLTN
                     LTLQVFGGFLRTQGTLGLGTPTPPFTGTVALQGLQVGPALQAVGTDQVSLSGTANAEL
                     ALSGRGFTHPDLVKALAGTGRMSVREGKIEGINLLQQAAMLLKVVGVSLDNVKATAFS
                     TIESDVVIKQGLIGLQRVLIDSHDFQATGGGTIGLDQSLDMKLNLNLSQALSQKIAAG
                     SPIAKVAMTNGRLSLPLVITGSTQAPSYGLDTKMFAGKVQEQVKEKVKGAVGDLLKGS
                     AKPEDLKKQGKDLLKGLFGR"
     gene            72470..72559
                     /locus_tag="PK589_00375"
     tRNA            72470..72559
                     /locus_tag="PK589_00375"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72504..72506,aa:Ser,seq:gga)
     gene            72853..72952
                     /gene="ffs"
                     /locus_tag="PK589_00380"
     ncRNA           72853..72952
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK589_00380"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            73054..74934
                     /gene="dnaX"
                     /locus_tag="PK589_00385"
     CDS             73054..74934
                     /gene="dnaX"
                     /locus_tag="PK589_00385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNJ18075.1"
                     /translation="MDYQVSARKYRPGTFDDVIGQSHVVQTLMNSIATKRIAHAFLFS
                     GTRGVGKTTVARILAKALNCEQGPTGSPCNTCANCQEITQGTSVDVVEIDGASNTSVD
                     DVREIRENVKFTPFRGQYRVYIIDEVHMLSNSAFNALLKTLEEPPSHVVFIFATTEIH
                     KIPATILSRCQHYNFRRISKAEIVQRLRHVADQDGLTIEDRSLMALARASEGSMRDGL
                     SLLDQVIAFGGKTIRHHDLETLLGAVPQERVRAMVEAVIDQNSAKALQVIAGLLDQGH
                     DVRAYCADLVEYVRNMLVAAVVPAGPELRGLIEVTDEDLTQLARDAEQFSVEHLQELF
                     RLWAAAEDHLRVSAHPRFVLETAAVRATRLLRAPESPAGLPGVNVQSSATPVDRATGT
                     RTSMSSSEKVVSPKPTKRDTVKSSSDAGDKPVVPSASAPAPAPVARPREAVVPSSQTP
                     APSIPSAQASATAQVPVESASPKPEPPSHRATASPSAEINWEEFQEAVSGQHPNIAPF
                     LEMGRVVKIEGGLVTLGFTKQATVARAMLEKEDNLQSLAALGERIYGTALRVRIIEVA
                     EQDPEATPTMKQLRVAKEQEQRVILTQQAKAHPLVKQALEMFGGELAEVRTTTPAQEV
                     QE"
     gene            74931..75254
                     /locus_tag="PK589_00390"
     CDS             74931..75254
                     /locus_tag="PK589_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010330208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNJ18076.1"
                     /translation="MKSPFGNMSNILKQAQAMQEQMAKVQEQAATKTVSGTAGGGIVT
                     VTVNGAMDLLSVKIDPEVVKSGDVDMLQDLVVAAGNDALKKSRDMMAEEMKAVTGGMK
                     IPGLF"
     gene            75333..75935
                     /gene="recR"
                     /locus_tag="PK589_00395"
     CDS             75333..75935
                     /gene="recR"
                     /locus_tag="PK589_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNJ18077.1"
                     /translation="MSIDQQGLLAKLVRELVRLPGIGQKSAQRLAFHLLKAEREDALR
                     LAEAIQAVKDGLSFCRQCRNIAEGELCEFCRDSKRDRSKILVVEEPSTLYAIERAGGY
                     RGLYHVLLGVLSPLDGVGPSDIRAEELLERVKAGGVEEVIVATNPTIEGEATAIYLTR
                     LLKPHHVRVTRIAYGIPVGMDIEYADEVTLVKSIEGRRDL"
     gene            76096..76503
                     /locus_tag="PK589_00400"
     CDS             76096..76503
                     /locus_tag="PK589_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNJ18078.1"
                     /translation="MKTSAKKSTPTRKKPAKANGVKYPDIQKDLEGQRAAILAEAGVV
                     LTNPTGLEVFPDVSDQASAEADQHFSFRIRERERKLLKKIDEALGRLATHTYGICEGC
                     QGDIPYKRLKARPVTTLCIECKTSQEEAEKSPR"
     gene            complement(76570..77715)
                     /locus_tag="PK589_00405"
     CDS             complement(76570..77715)
                     /locus_tag="PK589_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18079.1"
                     /translation="MHRRLSLLPALLTMFALLTGCETPPPARAPLPPSESDLDLLKST
                     KLCDTRKVFQEHHPGIVPKTQAWGSGQELRFSAEQSLSKGEESYFFDEDGTLVGTLFI
                     FRSGLDLGPYKTLRYTLTQLKPSLEFYLTVAQLADRQNMASSTLYDTGDEKTTTRYLV
                     LGDRKNPQLLAASFSIDPYVKLFSPYRKEFLDRLRDTGQQSGGQHLDTQGAEDKEPFA
                     SLQQFARGQTAQLAYCGAKNQTIALDAYQKASASGFTSQVWQAELHHRLGVSWEAAGD
                     LEKAKRELLASLAQRPNSPEVVNNLGYVYGKLGEKQNALASFEKAVLMRPNYAIARFN
                     LAEAIAEANPRRAINEYETYLALTEGIPEEADRSALAQSRVKALKHQ"
     gene            complement(77866..78195)
                     /locus_tag="PK589_00410"
     CDS             complement(77866..78195)
                     /locus_tag="PK589_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18080.1"
                     /translation="MRGTLATIMLGLLLTTGCSTTNQVATQDQNYNAPLSIYSAMDST
                     SLVYSDPMAASQANDNPYRWLGFVLHPVGHLVDYTVNRPLYAIARHAPYLFGYTAEDS
                     MIDSQRR"
     gene            complement(78756..78830)
                     /locus_tag="PK589_00415"
     tRNA            complement(78756..78830)
                     /locus_tag="PK589_00415"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78796..78798),aa:Val,seq:cac)
     gene            79016..80332
                     /gene="trmFO"
                     /locus_tag="PK589_00420"
     CDS             79016..80332
                     /gene="trmFO"
                     /locus_tag="PK589_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNJ18081.1"
                     /translation="MREDIVIIGGGLAGSEAAWQAAHRGAKVTLYEMRPKETTKAHKT
                     GDLAELVCSNSLGSMDPLNAPGILKGEMRRLNSLVIRAAEEARVPAGSALAVDREIFA
                     RAITQALEGHPNIRIIREEVTEIPADAVAIIATGPLTSEKLSKAISELTHERHLYFFD
                     AISPIIDADSINMDIVYRASRYGKGGADYLNCPMDEAAYNALYDAMMAAEKVQPKEFE
                     KVAYFESCIPIEVMAERGRQTMQFGPLKPVGLDDPKTGRRPYAVVQLRTENVHGTCYN
                     MVGFQTKLTYPEQKRVFRLIPGLEQAEFLRFGSLHRNTFINSPQLLRDTLQLKTRGTV
                     FFAGQLVGVEGYTESAAMGGIAGINAARGLAGQPLVTPPPTSAHGCLMAHITKSDPAH
                     FQPMNTNFGLFPPVTVKTRDKDQKRRLIQQRAVEDFDAWIAQSGLS"
     gene            80305..81267
                     /locus_tag="PK589_00425"
     CDS             80305..81267
                     /locus_tag="PK589_00425"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNJ18082.1"
                     /translation="MDRAIRAFLDVLAVQDDASPQTIRAYTSDLAQFRAFALAALKPR
                     DTLTVESIEAGLIREFLASRDRKGEKKTSLARKLACLRSFFRYLVRVGRVQANPAEDV
                     RAPKLPKHLPQVLTKDDAGALMEFPPGDAREGLRDRAILETLYSTGARVSELVGMDRQ
                     DISRSEGVVRVRGKGGKERVIPLSPLALEAIDAYHAQASVTAASNSSDRGAPGGSPVF
                     RNRRGGRLTTRTIARIVAKYSGQLSGGAIHPHTLRHSFATHLLDEGADLRAIQEMLGH
                     VSLSTTQKYTHLATDQLLALYDRTHPRAGAAGEAEQGNKQKGSR"
     gene            81264..81800
                     /gene="hslV"
                     /locus_tag="PK589_00430"
     CDS             81264..81800
                     /gene="hslV"
                     /locus_tag="PK589_00430"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNJ18083.1"
                     /translation="MKIRSTTVLCVRRGNQVTMGCDGQVTVGTTVMKHNARKMRRMHN
                     DTVLSGFAGATADAFTLFEKFEAKLAEYRGNLTRAAVELAKDWRTDRVLRRLEALLAV
                     ADLDHSFIISGTGDVVEPEDGILAIGSGGPYALASARALLAHSELGAEPIVKESLMIA
                     GGIDIYTNQQIVIESLTR"
     gene            81814..83217
                     /gene="hslU"
                     /locus_tag="PK589_00435"
     CDS             81814..83217
                     /gene="hslU"
                     /locus_tag="PK589_00435"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNJ18084.1"
                     /translation="MTTDSTSRSLNVNNLTPRQIVEALDRYVIGQQDAKRMVAIALRN
                     RWRRQQLAPELRDEVMPKNIIMIGPTGVGKTEIARRLAKLAEAPFIKVEASKFTEVGY
                     VGRDVESIIRDLTEQAISLVKTKHLEDVQEKASRLGEERLLDLLLPGGPSRPASAGFD
                     HAGSRAEAAEPTESPDATRSKLRLQLRDGKLDQRSVEVEVKERALPLGVISNQGGMED
                     LEGNLRDMLGGMFQGKKKKRVMKVPDALKHLTQEEAQKLIDMDEVVREAITKVEQTGI
                     VFLDEIDKIAGRERAMGPDVSREGVQRDLLPIVEGSTVSTKHGAVQTDHILFIAAGAF
                     HVAKPSDLIPELQGRFPIRVELAPLTKEDFVRILTEPRGALVRQYHALMATEGLTVEF
                     TDDGLAEIAATAVQVNERTENIGARRLFTIMERLLEQVSFEGSELEQKAVVVNAAYVR
                     ERLLNIVKDQDLSRYIL"
     gene            83247..84143
                     /gene="argB"
                     /locus_tag="PK589_00440"
     CDS             83247..84143
                     /gene="argB"
                     /locus_tag="PK589_00440"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNJ18085.1"
                     /translation="MNKLIKKADVLIEALPYIRTFKGKTVVIKYGGHAMTDASLKARF
                     AQNVVLLKYVGLNPVVVHGGGPQIDQMLDRLGMEAKFKHGVRVTDAATMEIVEMVLAG
                     RINMEIVDLLNRHGGQAVGLSGKDGGLMLTKPLTAKAWAESIEKDIDVEDRDADFGFV
                     GDVKSIDPTLLLKLQEDHYIPVIAPIGTDREGNTYNINADLVAGAIAAALKAEKLVML
                     TDVKGIRDANGRHLSTVSRKDVQRMVKRGTISEGMLPKVHACLDALAGGVGKAHIVDG
                     RVPHAILLEIFTHKGIGTEIVS"
     gene            84372..85070
                     /locus_tag="PK589_00445"
     CDS             84372..85070
                     /locus_tag="PK589_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18086.1"
                     /translation="MARKRMSVGPATRRAIKDIFSRLDYAQLGPIYCDEGGDEFWAAK
                     RGPCQRLGTKVATALKARLKPGGTSVYVGAGVAEIPPLVMEALELDRQVVACNLRRQE
                     VLLLNRACGELPIRFQHVDAGSVKQRVDHLWLVSVLNDPERFPELSVLSYGRANPVTF
                     NAKKFVTQQRTVARLVDRCLSRLTVPGLVTTTVEEAVWVAEWCHRRGIPYRIDERTYA
                     SPTVGDPICLMRIG"
     gene            complement(85101..85640)
                     /locus_tag="PK589_00450"
     CDS             complement(85101..85640)
                     /locus_tag="PK589_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNJ18087.1"
                     /translation="MIRTFQGIKPTIPQSCFIEETAVIIGDVVMGEECSAWFHAVIRG
                     DVNSIRIGNRTNVQDLCMLHVTHDTHPLIIGDDVTIGHHVVLHGCTVQNRVLVGMGAI
                     IMDGAVIGEDSVVGAGALVTEGTIVPPKSLILGSPAKVKRPVTEQELAWIRESAQNYI
                     RYSRQYLTGPDKPRPGFWV"
     gene            85725..87179
                     /gene="rimO"
                     /locus_tag="PK589_00455"
     CDS             85725..87179
                     /gene="rimO"
                     /locus_tag="PK589_00455"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNJ18088.1"
                     /translation="MASSLITPSRAKGKKPTIGFVNLGCSKNQVDSEVMLGTLVAGGF
                     QLTGDARAAEVVIINTCGFIEEAKQESINSIIEHGRLKKSGSCRVLIAAGCLAQRYQG
                     ELLKELPELDGVVGTGEFGRIADICRSLLAPKKRQQRLWLGQPPYLYDADTPRVRLGT
                     PHSAYLKIAEGCNRNCAFCAIPIMRGKQRSRPIDSIVAEARRLAKEGVKELNLISQDT
                     INYGVDLGLKQGLTALLRELVTVDDVRWIRPFYLYPQQVTDELLELYAGEARITKYLD
                     MPLQHISDGMLKRMHRLGDRKHLTKLVERIRTKIPGVFFRTAFIVGFPGETDKQFEEL
                     KQFVLDMEFDRVAVFLYSDEEGTSAVDLDRKVDQAVMEERRNELLALQESISESKNRE
                     YLGRTIEVLVDGVSEESDRLLEARHQGLAPEIDGVVYCERGIARPGEFISVTVTDVAG
                     YDLIAQPAGQTHQPNRSSDSPGLLMPKKAGAGYR"
     gene            complement(87222..88700)
                     /locus_tag="PK589_00460"
     CDS             complement(87222..88700)
                     /locus_tag="PK589_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNJ18089.1"
                     /translation="MKRPTPSVSVLAGIAAVSAGLIWSYTPLSPSHASNPSPVVEERP
                     IAPTGALPAVGFADVAKTVTPAVVNITTSGAEEVSDHGRPRGKMDDFFGSPFGPRRFG
                     PPTEPREHRGGGQGSGVIVTQDGYVLTNNHVIAGAKTVTVTLPDKREFKGRIVGSDPK
                     TDIAVVKIDGTQLPTVPWGDSSRLQVGEYVLAVGNPFGLNSTVTLGIVSALGRGHMGI
                     TQYEDFIQTDAAINPGNSGGALVNTKGELIGINTAIFSQTGGYQGVGFAVSTGMSKPI
                     YESLVKTGKVVRGYLGIGIQDLNHDLAKSFGVKGTNGAVVTDVKEEGPADKAGIKQGD
                     VILSFQGTPIEDAVTLQRSVTRTAVGSKATVTVLRDGQEKELAVTLAELPENQQVAKT
                     ETEPSDQPLAGLAVQELDRETAQELGLKGKIQGVVVTTVDPESDAERAGLMPGDVIRE
                     INRKPVTSMKEFDRAASSLKKGQTVLVLINRRGASLYLSAKI"
     gene            complement(88819..90222)
                     /locus_tag="PK589_00465"
     CDS             complement(88819..90222)
                     /locus_tag="PK589_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ18090.1"
                     /translation="MPLRVRLTLWYGTALALILLTFSVVLYTITARGLRDQVDESLQE
                     TASAAVRSLETRGFLPLIDEDALLSQFPELTRIDKFFQIFSPTGTITIRSPNIRQHDV
                     PLSRPALEAAFTGSTVFESARYPNEPPLRLVSVPIIYRGSLLYIVQVGTTLEGVEETL
                     RRFLLVLLVMAPIALVVSLVGGWFLAGRALRPVDSITVAAQRIAAGDLTQRLHSERST
                     DEIGRLTDTFNNMIARLETSFAQIRQFSSDASHELRTPLTVMKGESELVLRRPRPVED
                     YIAVLESNLEEIDRMSRIVEELLFLSRADMGQVKTECEPVRLEALVEDIQRQACLLGQ
                     EREVDVIMGTIQPATVRGDELRLRELILNIVDNAVKYSHPCGKVEIDLRVEGSTARLA
                     VRDYGIGIPQDAHKQIFDRFFRTDDARAHTKKGTGLGLAICAWIAEAHHGHIEVQSEV
                     GKGSTFTLVLPLAATAA"
     gene            complement(90222..90893)
                     /locus_tag="PK589_00470"
     CDS             complement(90222..90893)
                     /locus_tag="PK589_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ18091.1"
                     /translation="MRVLVVEDETKVGSFIKRALEEESYAVDLCEDGAQGLDLALTGS
                     YDLIMIDLMLPGLPGMEVLTRLRKEKIQTPVLILTAQSKVDQRVKGLDAGADDYLTKP
                     FAIDELLARVRALLRRGTAESSGTLQIDDLILNPATREVTRGGQRIDLTVKEYALLEY
                     FMRHAGRVLTRPMISDHVWNQDFDTFTNVIDVYVNYLRNKIDRGRARKLIHTIRGSGY
                     MLKVD"
     gene            complement(90962..92104)
                     /locus_tag="PK589_00475"
     CDS             complement(90962..92104)
                     /locus_tag="PK589_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNJ18092.1"
                     /translation="MSTARTAHYLDGRTATRHRVTLTISPAALQIAMPDGSTKEWPYG
                     QIRQTQGTYRGEPVRLEFGTEPAEAVVVASRALLTDIHTAAPALAQHFHDPSWRQRRL
                     AWTLGAGLGVIVMLIGLYRWGIPGIASAATPYVPIMWEESLGRQIVEHLAPATQQCRE
                     PERLRKIDQVVQMLAATHPDSPYRITLSVVDNPAINAFAAPGGHVVVLRGLLEQTGSA
                     EQLAGVLAHELQHVYLRHSTRAILEQTAGTLLLTAVSGDLSGGLAWGLQGAQTMGSLH
                     YSRTHEEEADVEGLRMMQAAHLDPTAMIAFYGTLQKSEQDHIEPPDFLSTHPDMSRRL
                     ATLLALAGPPPTDARQLLPGEDWKDIRTLCRLQTATRSSSDSPDLP"
     gene            complement(92107..93129)
                     /locus_tag="PK589_00480"
     CDS             complement(92107..93129)
                     /locus_tag="PK589_00480"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HNJ18093.1"
                     /translation="MPAPMRASFHGMGGTLLGMHVVNVCLTLVTLGGYHFWAKAKIRR
                     YLFSHTAFAGDRFAYHGTGKELYQGFLKAMLVFGIPYFSLSAAHTFLELPQWIDVLLQ
                     VMAGLVFFLYVPVAIVNARRYRCTRTSWRGIRFSFRGRTSDFLKLYFRGWFFTILTLG
                     TYYPYFQTQRQAFLHSHTYFGNQRFRFTGHGSGVMVPFAITLFTTYAVLALCGFALAA
                     QLTNAGLTLLLIPCVLGPMWIWLLGQKQRYFWNHTSFGDAQFSSEITWQRLFTLYLGN
                     LGLLLATLGWAWPWVTVRNARFFMGTLSLQGPVDLDRVLQDTSEASVTGEGLSNLLDT
                     GFDMDA"
     gene            complement(93382..94692)
                     /locus_tag="PK589_00485"
     CDS             complement(93382..94692)
                     /locus_tag="PK589_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ18094.1"
                     /translation="MTTVHPGTALPASPTLTASDAAQTAICPFCQAITSCAPVAGHRL
                     HQFPCPSCHANLLVLNPAARPTDEDKKTRSSDGDLPIDLSHRLTGIAVAVLACALNYV
                     LLMGMAYWILGQVKTTHDPYDIDALMLSPLILKANGWTPLHLAAARGDQSAVATLLTE
                     GQTVDQRNGNGRTALFEAAKRGQTDVVTLLLQHGANPSAKGKQGFTPLLAAAEQGHAD
                     TIEVLLTNGADIRATCTCGDSALHRAVRHGHLAAAQTLLEGGIPVNQKSHGETPLEIA
                     QHDEDHELIALLRAYGGREFSDAKAKRAEGIAFQKHGQIDHALFAYAEALNLDPDDHE
                     AYYGRGTALLQKNAPDEALIALHAAIRLNPTYLDAYSAAASVHTSRGQWDQALRLWDQ
                     LLSHQPQNGRAIFERSIVKRGKGDSAGFLQGLQQACALGHRAAC"
     gene            complement(94868..96520)
                     /locus_tag="PK589_00490"
     CDS             complement(94868..96520)
                     /locus_tag="PK589_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNJ18095.1"
                     /translation="MTHDILVVGAGLAGMRAAIAVPPDLNVALLSKVHPIRSHSVAAQ
                     GGINAAIGAEDSWEAHAYDTAKGGLYLGDQDAIEAMCKEAPQDILELERMGVIFSRTA
                     EGRIAQRPFGGAGYPRTCYAADRTGHALLHAMYEQLMKRRCKVYEEWYVTALLVEEGR
                     CCGVVAWDMIHGGLQMLRAKAVILATGGSGRIFSTSTNAVINTGDGMALAYRAGIALE
                     DMEFVQFHPTTLKDTGILITEGARGEGGYLLNTLGERFMKRYAPEQMELATRSTVSLA
                     IGQEIQEGRGVDGCVLLDLRHLGRAKILERLPQIRELAMEFAGLDPIETPIPIRPGAH
                     YQMGGVRTNAWGETELPGLFAAGECACVSVHGANRLGGNSLLETIVFGRRTGTKAAES
                     IRTVQLSPVSETILKSEQQRIDKLLSNPGPERAWQIRDDLGKTMSLNLGIFRTKQSIQ
                     EALSAIQTLKRRAEDMCVQDKGRVFNTDLIHALELQCLLEIAETIVVGALGREESRGA
                     HYRGDFPTRNDTAWLRHSLSYRRPEGPHLAYAPVTITRFPPA"
     gene            96728..99613
                     /gene="gcvP"
                     /locus_tag="PK589_00495"
     CDS             96728..99613
                     /gene="gcvP"
                     /locus_tag="PK589_00495"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNJ18096.1"
                     /translation="MPEPQFLEPNDTFVPRHIGPTESEIQTMLETVNVPSLDSLVEAT
                     VPSDIRLQGSLAVPSPRGEQEVLAELRGMAGQNQVWRSLIGMGYYDCITPPVIQRNIL
                     EGPGWYTQYTPYQAEIAQGRLEALVNFQTMVADLTGLPLANASLLDEATAAAEAMTMC
                     AAISRAAGHDRHKFFVSETCHPQTIAVVQTRAEPLGIVLQVGAIQSLDLSSGEFFGML
                     LQYPSTDGYVGDYSEFITRAHAAGVYVVVATDLLALTLLRPPGEFGADVAVGSSQRFG
                     VPLGFGGPHAAFMATKEEFRRQMPGRIIGVSKDVTGRPAYRLSLQTREQHIRREKATS
                     NICTAQVLLAVMAGMYAVYHGPAGLRRIAERIHGLTMALAEGLRRHGCAVGLEPVFDT
                     LRVPLSPAQSETIVNRARQQKINLRRYDDHSLGLSLDEWSTVDEVRQLLTLFAGQEIS
                     PEELQTILESVDGRYPAGLARTSQYLTHPVFHRYHAEHELLRYIHRLQSRDLSLVHSM
                     IPLGSCTMKLNATAEMLPVTWPEFGRLHPFAPADQVQGYLALFQQLESWLAEITGFAG
                     ISLQPNAGSQGEYAGLMVIRAYHRQRGETQRDICLIPVSAHGTNPASASMCGMTVVPV
                     ACDQRGNVDLADLEAKATQHRSRLAALMVTYPSTHGVFEEGIRRMCQIVHTHGGQVYM
                     DGANMNAQVGLCRPADLGADVCHLNLHKTFCIPHGGGGPGMGPIGVARHLMPFMPGHP
                     VTKLGGPQSIGPVSAAPYGSPSILTISWVYIALMGREGLTKATQVAILNANYMAKRLE
                     KFYPVLYSGARGFVAHEFILDLRPLKDSSGVEAMDVAKRLMDYGFHAPTVSFPVAGTL
                     MIEPTESEVKSELDRLCDALIAIRGEIQAIAEGRQPRSGNVLKNAPHTAMAVTASEWA
                     RPYSREEAAFPASWVRENKFWPSVGRIDEAYGDRHLFCTCPPVDAVS"
     gene            99648..100250
                     /locus_tag="PK589_00500"
     CDS             99648..100250
                     /locus_tag="PK589_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18097.1"
                     /translation="MGRVRRVVRQIAIGCLLLNMFFALPVHATVESVAVADTHIEDSP
                     LDVVSGLTYRAGTRLRIPGTGWSFVVPDGWQSNRPDDAEMPFLIPEEGKELGMMFPLA
                     KVTRDALREQLREPLLLLHGLSFIPAGAQAETDAWIAQSYEGEGMAGRALAVMGADNT
                     AVIYFLMGPPHNMSVLQSVLEQLGQSTRFVQSTPRRDAGL"
     gene            100327..>101074
                     /locus_tag="PK589_00505"
     CDS             100327..>101074
                     /locus_tag="PK589_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNJ18098.1"
                     /translation="MQKTSPTKVIIDTDPGVDDALAILLALASPELDVVGITTVCGNV
                     PVGQGTKNLFRLLNLLNPPPGLLVGQGAARPLEEALETAVHVHGSDGLGELDALLSAE
                     GTMRYPAVRLPPLLSTAQDVWNECVRRYQDDITLITLGPLTNVAVALKVNPLTVQKFR
                     SVIVMGGAIGVPGNVSPVAEFNMYVDPHAAHRVFQAALPLTLVPLDVTTRVGLRRDVL
                     KMWTGASRDPRCRIVADMTAKAFDFAEQVEG"
     assembly_gap    101075..101174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <101175..101474
                     /locus_tag="PK589_00510"
     CDS             <101175..101474
                     /locus_tag="PK589_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNJ18099.1"
                     /translation="IVADMTAKAFDFAEQVEGHGLLYFHDPMAVLAAIEPSLLKTEPL
                     HVEVEVVGRVSRGATVADRRHRRPEEKANPNMQVAVDVDAERALSVIRSRLCPWS"
     gene            101465..102385
                     /gene="rbsK"
                     /locus_tag="PK589_00515"
     CDS             101465..102385
                     /gene="rbsK"
                     /locus_tag="PK589_00515"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HNJ18100.1"
                     /translation="MVVVVGSSNIDFTAVVDRLPARGETLLGHRFLQSFGGKGANQAV
                     AAARAGADVAFLSKVGNDFNGDLIAQHLATQGLSRLMLLRDAAVQTGVAMILVDVAGD
                     NQIVVVPGSNQQLTPADVEQHAQLIADARVLLVQMEIPIDTVQQCLRLAKRYGLRTIL
                     NPAPACPLPPESLRMVDILTPNEREACVLSGATDPADAARVLVSTGAETVVVTRGTEG
                     AVVSRGKHVTHIPAFIVDAIDSTGAGDTFNGALACGLAEGLSIEEAVEMAAAAGALAT
                     TGYGAQAAMPARGDIDRLRRTGVRRMPPRG"
     gene            complement(102382..103023)
                     /locus_tag="PK589_00520"
     CDS             complement(102382..103023)
                     /locus_tag="PK589_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18101.1"
                     /translation="MNYPVIALWGLLGVALIGEQAVAGTAVQRLTAESMHECSLGRQA
                     QTRADRIQHFASGQAFGEQAVAADESSPDAHFALFCNLGEQLRVDGESLSSLFGFRRM
                     MKELNRTLELDPSHLDALSAKGTVLVRVPGFMGGDKEKGELLLRQVIAREPAAVNARL
                     SLAKSYCADGRHEEAVAIATKALDLAQELQRADFIPEARQVLAQLRAQSTKGN"
     gene            complement(103130..103660)
                     /locus_tag="PK589_00525"
     CDS             complement(103130..103660)
                     /locus_tag="PK589_00525"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HNJ18102.1"
                     /translation="MPLSLPDHLAPNLNILFVGINPGLRSAAVGHHYAGHSNRFWKLL
                     YDAALVPEPLTYHDDERLLEWGLGLTNLVARPTAGSEHLTAEDYATGRRALMEKLRRY
                     RPRVTALVGITLYPILFPLEPKQAGRKPGLQSVTLYDSSIVLLPNPSGRNAAYPYHAL
                     LDAFCTLTPWVRTSLG"
     gene            103937..104161
                     /locus_tag="PK589_00530"
     CDS             103937..104161
                     /locus_tag="PK589_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18103.1"
                     /translation="MKKMMLTVMAAALLVAFSAPSFAGDDKKKEEKKGGHFSQTLFGD
                     DKKKEEKKGGHLSQTVFGEEKKKDEKKGGH"
     gene            complement(104238..104756)
                     /locus_tag="PK589_00535"
     CDS             complement(104238..104756)
                     /locus_tag="PK589_00535"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNJ18104.1"
                     /translation="MVRPIYKGTVVTLNVDTVRLPNGHTIDLEVIRHPGASAVVPLKK
                     DGTVVLIRQFRHAANGFIYEIPAGKLHPNEDPLDCAARELEEEIGYKAGRFQLLSSIF
                     TAPGFADEVIHVYLATELTLGTQNLDQDEVLDVVEMPLKEAIDRIEDGTIRDAKTIVG
                     LQAVYIRQVGNR"
     gene            complement(104769..105878)
                     /gene="gcvT"
                     /locus_tag="PK589_00540"
     CDS             complement(104769..105878)
                     /gene="gcvT"
                     /locus_tag="PK589_00540"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNJ18105.1"
                     /translation="MKQTPLIDAHRNAQGKLVDFAGWEMPIQYSGVVDEYQTVRRHAG
                     LFDVSHMGRIRVAGPGSLAFLQRVTTNDVSKLSVRQSQYSMVCAPNGGIKDDIFIYHV
                     KPYEFLVCVNASNREKIVAWFHEKVAQAQGCTVQDQSTTLAQIAIQGPASRDILTAVG
                     LTDLPLLKIRHCLDAVVCGHATLVTRTGYTGELGYELYLPAEGAMKIWNRLLEAGHPL
                     GMKPAGLGARDLLRLEMAYLLYGNDMDEGTSPIEAGADWVVKFDKGEFIGRTPLLAQH
                     NDGPSRRLVAFELVEKAVPRHGFKILSTQDQHAVIGEVTSGNLSPLLQKGIGMGYVTP
                     AASQPGSSILIDIRGKSCPAVVVKPPFYKKQPSTP"
     gene            complement(105935..106309)
                     /locus_tag="PK589_00545"
     CDS             complement(105935..106309)
                     /locus_tag="PK589_00545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNJ18106.1"
                     /translation="MMANSEQPGTTAEVTTASGLKYVDVVVGTGREAAAGNLATVHYT
                     GWLTNGKKFDSSVDRRDPFSFPIGAGQVIRGWDEGVAGMKVGGKRKLTIPPELGYGAR
                     GAGGVIPPNATLVFDVELLEVR"
     gene            complement(106433..107653)
                     /locus_tag="PK589_00550"
     CDS             complement(106433..107653)
                     /locus_tag="PK589_00550"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNJ18107.1"
                     /translation="MTAPSTLRFASALTRHADAQAAAADLIHAIREQLGPSRIDVAFL
                     FISAQHADQAEALSEALRTALGPVTLVGCTGEGIIATGREIESGPAATLWAAHLPGVL
                     VQPLRLSFSSVHDQFSLRNWPDLDEVGDTAPVMMLFADPFSTPMQEVLSVIEEQYPGT
                     RTLGGLAGGGQDLGENRLFLDDQVLTDGLVGVALSGNIAVRSVISQGCRPIGDRFIVT
                     KSEHNVIQELGGMPALHCLQTVFEQLSSDERAQAQRALHIGIAMDEQRAQFTLGDFLI
                     RNLLGADQQTGAIVIGDVIQEGQTVQFQVRDAHSADEDLHALLAAPPQGVPSKPLGAL
                     LFSCCGRGKGLFGVPHHDASVLGEQLGAIPLAGFFAQGEVGPVGGRNFLHGYTASIAI
                     FSEPERGHQTGLNR"
     gene            complement(107705..107932)
                     /locus_tag="PK589_00555"
     CDS             complement(107705..107932)
                     /locus_tag="PK589_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18108.1"
                     /translation="MADYSMGGGLTLLTALEKSDKFADFLKTRMAQALETRDPTELHY
                     LLAQLDDYHSYMWRYYKKLATERPERMNPGV"
     gene            complement(108020..109285)
                     /locus_tag="PK589_00560"
     CDS             complement(108020..109285)
                     /locus_tag="PK589_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNJ18109.1"
                     /translation="MSHSHPGSFSIIACVVLVMTTGVHSGWSAETLSDVTEEPRVRAR
                     ALGLTLGQLEPGPLNAITDVPGVKVGQVTLRRGEGPLQPGQGPVRTGVTVIVPREDVW
                     HKKVPAGAFVLNGTGEMTGLAWVAESGFLEYPIALTNTLNVPRVANGVISWMLRRYPG
                     IGITDDTLTPVVAECDDGRLNDIQGRHVSEADVMQALDDAASGPVSEGTVGAGTGMIS
                     YGFKGGIGTASRRLPAAAGGFTVGVLVNANHGRRPELMMGGVPVGQRYDAAPTQSGAL
                     LESNPERLHASREGSSGNAEGSIIIIVATDAPLDSRQLTRLGKRAALGLARTGSTARH
                     GSGDFMLAFSTGNVIPHYPTEPTFSLTHLADTHLNAVITATVEATEEAVLNALTGATT
                     VTGRDGFRAEAISLPRLRTLLSPDPAIGR"
     gene            complement(109334..110731)
                     /locus_tag="PK589_00565"
     CDS             complement(109334..110731)
                     /locus_tag="PK589_00565"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ18110.1"
                     /translation="MKISFHGAARSVTGSRHVLEMPGSRILLDCGLFQGPRQEADRQN
                     RFLGFDPRSIQAVLLSHAHIDHSGALPVLGQHRFRGDVHMTRATADLTAVMLEDSARL
                     QENDCAYLNRKEHRRGQKCLKPFYETRDARAIIRRFVGARYGDTIKVTPRLTASFHDV
                     GHILGSAAIRLKYSARGNSTTVLFSGDLGRSHMPILRDPTPPPSCDVLIIESTYGDRL
                     HEEAGETLTKKAQQLVAHAKAHNSKIIVPAFALGRTQDLVMRIKHLVAEGQIDPLPIY
                     IDSPLASKVTDVFRKHPECYDEETYRTFTSEGDPFAARYIRYVSSPDESKRLNSVKGP
                     CVIIASSGMCEGGRVVHHLKHAIQDEANIIAIVGFQAEHTLGRRLVEGWDVVPLFGIP
                     TARRAQVVVFNGLSAHADRNDLLAYVRAISPPPQHIFVVHGEEKQALSLAAAIQAEYP
                     RTSIVVPHKESSYEV"
     gene            complement(110728..111585)
                     /locus_tag="PK589_00570"
     CDS             complement(110728..111585)
                     /locus_tag="PK589_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNJ18111.1"
                     /translation="MGAGSSDESASRHPHSASSSYIPADRDIDFLQRDELRPLRLGLE
                     LLKPELIQIEQGIRSTIVVFGSSRLVEPSLARQALEAAKAELQIAPQDALRQQQVTMA
                     ERRLAMAHYYDIAREFGRLVSATCQINGQCDFVIVTGGGPGIMEAANRGAADAGAKSI
                     GLNITLPHEQQPNPYITPDLCFQFRYFALRKMHFLLRARALVVFPGGFGTLDELFETL
                     TLLQTGKTRNITIVLIGRAYWERLINWSMLADEGLIGPNDLALFHFAETAQQAWDLIS
                     QTHGGPAAR"
     gene            complement(111651..112025)
                     /locus_tag="PK589_00575"
     CDS             complement(111651..112025)
                     /locus_tag="PK589_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18112.1"
                     /translation="MMPLSGCNVARLTINTPLTPQTVSFITEGQTTLTDVVNKLGAPD
                     SITDAEGGAVVTYRFLNAKYSRVNFGWLVKPWSPVDPDLVFSRSGMGTDAFELLCDSN
                     WVVTHQAFVRHRPDPLFTPYPF"
     gene            complement(112078..112458)
                     /locus_tag="PK589_00580"
     CDS             complement(112078..112458)
                     /locus_tag="PK589_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18113.1"
                     /translation="MRRAGLLLGAMLLTLNACAFSRGTLGEEIKTDTVNSIKKGVTTR
                     ADVLAQLGAPDRLLQLNGRDLFQYYRYDAKVGSLLLILINFSRVSVKSDDLFVIINRD
                     GIVEDVIASKRTENLEFRFWPFGD"
     gene            complement(112496..113011)
                     /locus_tag="PK589_00585"
     CDS             complement(112496..113011)
                     /locus_tag="PK589_00585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ18114.1"
                     /translation="MISADQLRIRLATVQDVDVLTTFSAAMAWETEHRRLDLARLRLG
                     TQAVIDRPEQGQYYVADLHRNAPADTVTVGQLLLTYEWSDWRNAQFWWIQSVYVDPAW
                     RRQGVYRAMHQAVMALAQSRADVCGVRLYVEGDNTVAQGVYERVGLTPSTYRIYESDF
                     VLPKPSSPDRR"
     gene            complement(113008..113634)
                     /locus_tag="PK589_00590"
     CDS             complement(113008..113634)
                     /locus_tag="PK589_00590"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNJ18115.1"
                     /translation="MTSSHRLGRIFFTGLLLLVPAWTTFLILAALFETLDSFLLNLIG
                     HQIQPYAPGLGILLLIGMVLMVGAIATQVIGQRFVRWAETTLEGIPLIRSIYLTLKSM
                     TDLLNYRARFGQSTVVAFPFPRDGLWALGFVMGPPPAALQVAPSVELVMVFVPTAIHP
                     FTGYLAMIPKTQLQPLNLLPEEALKLEFSAGLYRPLQGWLTAPAVKSS"
     gene            113777..114073
                     /locus_tag="PK589_00595"
     CDS             113777..114073
                     /locus_tag="PK589_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNJ18116.1"
                     /translation="MRFIVSDAVQKKVNLDKDLLAILCCPETKQAVVLADDLLIQKVN
                     GAIERGVLKNKAQKAVTEKLDGGLVRSDNKILYPVREDIPVMLIDEGIPLDQVA"
     gene            complement(114584..114817)
                     /locus_tag="PK589_00600"
     CDS             complement(114584..114817)
                     /locus_tag="PK589_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18117.1"
                     /translation="MAREWIIFAICLGLGGHIALAVVLHAPDMWPWSKAWLYGLLSGL
                     TLYIVVQVLRSLWWVFRGRNGDEPDVPEDQHIR"
     gene            complement(114875..115243)
                     /locus_tag="PK589_00605"
     CDS             complement(114875..115243)
                     /locus_tag="PK589_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18118.1"
                     /translation="MNPTLQRAVTSFYNLVYEAQTFATTMSRIDTAEQEHYAGRIEGL
                     NWVLDRCQELEDMDANITPTTLQRVLTEVKSDLDHELSVQRREKGRRADGREEALTFV
                     ADYLSSLITATEIESAKTSV"
     gene            complement(115327..115656)
                     /locus_tag="PK589_00610"
     CDS             complement(115327..115656)
                     /locus_tag="PK589_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18119.1"
                     /translation="MTSRPQQSAPFAAQAVSFDEYLASGKIPDGLLVSEYVEQQFVER
                     LVHYVLSVPAGSYSMAQLSRLLEQLDPRAQVFFFKRLKENSPDSLKDFAPLYYGFMNE
                     FHSLLFT"
     gene            115932..116006
                     /locus_tag="PK589_00615"
     tRNA            115932..116006
                     /locus_tag="PK589_00615"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:115964..115966,aa:Gly,seq:gcc)
BASE COUNT        24072 a        31547 c        35575 g        24617 t
ORIGIN      
        1 tcatggtcac acgagtcgac tgttcacaac caaggaggtt tcacatgcgg agtcatatca
       61 aggtgcagat gggaatagga gtactgctgg tggcgggggc gacatccttc gccattgccg
      121 cagatgccag caacgagaac aaggacatgg tgcttgtccc gaagggtgaa ttcatcatgg
      181 gcagcaatga acattccgat gaggtgcggc accaagtggt gctcgatgcc tacctgatcg
      241 acaagtttga agcttcgaat gcacgctaca aggaattcat gcgagccacg ggccaccccg
      301 ccccggccta ttgggatgat ccccgtctga gcaagccgga acagccggtg gtcggagtga
      361 gctggacgga tgcgaatgcg ttctgcaagt gggacggcaa gcgcttgcct accgaagcgg
      421 aatgggaaaa ggccgcgaag gggccggaag gcgataacca ttatccgtgg gggcatcacc
      481 tcgatccgaa gaaagccaac tatggacaga acgtgggccg cacgatgccg gtcgactcct
      541 atcctgaggg cgtgagcggg tatggcgtct acaacatggc cggtaacgtc tttgagtggg
      601 tcgacgactg gtatgacccc aaatactaca agaccagcaa cgcgttgaat ccgaagggcg
      661 cggacaaggg ctacaacttt gccaatcaag gcccggtgaa agttttgcgt ggcgggtcat
      721 ggctcgctcc ggaaacctca ttgcacacca gccaccggtt ctggaatcag ccggagaata
      781 actcctacgg cgttggactt ggcttccgct gcgcgaagtc ggtgaccgcg gtgtcggagg
      841 aagcggccca ggccggacgc gatgccttca ttcaggcact cgtgggcatg ggtgcggaga
      901 agaacgcaga tgcgatggcc tccatcgaga aggccctggc tgcggatccg aacaataaag
      961 aatacctggc aacacgtgag cttattcaga agtcgatgaa gaagtaaatg ctggccgcag
     1021 aggaggtggg atgaccacct ccctgtcgga ccttcagcag gaggaggtgc ggcatgactg
     1081 tcatgggaac ggtagtcagg gcgggactcg tcatggggat catcgcaggg gcttctgtcg
     1141 ctcaggcgca cgaggccgag attccgaagg atatggtgta cgtgggacaa ggcccgtcca
     1201 tcatgggact ggataggacg gaagcggccg caccgacccg tcgtctttcc ctgtacgaca
     1261 agcgaatgag catgccgtgg tcagcggagg ccttccacga cgaaggacct gcgcacacgg
     1321 tattcctgga ctcctatctg atcgataagt acgaggtgtc aaacaaagac tacggggaat
     1381 tcatcaaggc gaccggccac cccgctccgg cctattggga cgatccgcga ttgaacaagc
     1441 ctcaacagcc tgtggtcggg gtcaactgga tcgacgcgaa aacctattgc gagtatcgcg
     1501 gtaagcggct cccgacggag gcagagtggg aaaaggcggc tcgtggtcct aacggcaatc
     1561 tctacccatg gggcaacgac ttcgactcga ataaagcgaa ctatgatcgg caccataacg
     1621 cgacccagcc ggtagatgcc catcctgagg gcgccagcta ttacggcgtg tacaacatgg
     1681 cgggcaatgt ctttgagtgg gtgggcgact ggtacgaccc gaaatactac ggacgactgg
     1741 aaacgatggt gaaccccact ggcccggaaa aacccttgtg gcttggtggt accgggacct
     1801 atgtggatcg gctgacggtt ggcgagaaac gtgtgatccg cggcggttcc tggattgcac
     1861 cggaagggac gatccgctcg acgcatcgct tctggaatca tcctctcaac aacagctacg
     1921 gcgtagggct cggcttccga tgtgccaaga cggcgccacc agactgggag cagcgtatcc
     1981 gggatagtta catcacggca ttggtggaga tgggccggga acgtttcgcc gaggcgcaac
     2041 aagcagtggc tcgtggacta gccatggatc ccgtgaatgt cgaactgttg gagttgcgac
     2101 ccctcatcga acaatcgatg aaacgtccgt aacgtcggac ggaggactgt cgcacccaag
     2161 agtttcttat gacggaggca gaggtatgag gcacaggaca atcgtcacaa tcatcggtgg
     2221 gttggcggcg ctggtatccg cgatggaact tcaggcaggg ccgattgacg ccagccgcca
     2281 cactcatccg gagaaggtgc aactggtgca tgaggcggag cacagcgtgg atcacgcctg
     2341 ggaggtgtac catcgtgcgg cgctgggggg aaccgtggcg tcacccgatc tgcaagcaca
     2401 aatcgaacaa catttgcatg aagcgagaac cctcgtgacc aaagcgcagg aagcggccga
     2461 ccgtggcgac gcaggtgcgg tggaaaagct cgtcgcgcag atcaagactc acacggaacg
     2521 ggcaatagag gggagcaagg agcagaagaa atgacacaag gtttgaccag gagacgtacc
     2581 agatggggat atgcgggcct gttggcggcc gcctctacgc tcgcactgtc ccctgtgttt
     2641 gcgacgccgg ctgtcaaaga gctggatccg gttctcatgg taaccattcc ggccggaccg
     2701 ttcttgatgg gcacgcagag cccgaaaggg agggccgacg aatggccgca acgcactgtg
     2761 caggtggatg ccttcgccat ggacgaagtg gaagtcacga atgaacggta catgacattc
     2821 gtcacgacca caggccaccg gagtccgccc aatccctacg gaaccggtgt gctggtgtcg
     2881 gccaagggaa tcgaacaact gccggtcgtg caggtcacat ggtatgacgc gaaggcctat
     2941 tgcgcctggg ccaagaaacg attgcccacg gaagcggagt gggaaaaggc cgcacgtgga
     3001 accgatggcc ggacctttcc gtggggggag gacacgccga cgtcgaagcg agctaactac
     3061 gaccgcgagt gggtggagga caagacgctt caccctgtgg gggccttgcc ggctggcgat
     3121 tctccgtacg ggatcaagga tatgtcgggc aatgcccggg agtgggttca agactggtat
     3181 gacccggatt actacgcgaa tgctccgaca aaaaatcccc agggaccaga caagggtatc
     3241 gtgcgtgtga ttcgaggagg atcctggcat agcccgcatt cagacatcac ctcgacagca
     3301 cgaggccggg gaggattcgc gcttcaaaca cacgggacag ggtttcgctg tgtgcggagc
     3361 cttgacggtc aggctcacca ggacgcatcc ggcgctgagc cgaaagccag gtaagcgatg
     3421 ggttcatggg ggcattcgtt gaaacgagaa ctgtggtgag catggaggta ccgcgactcc
     3481 gcacgcggcc tcccgttgag gcggatgggt gcttcctttg gaggcacctg tcccctcagc
     3541 gaaccaccag agggtgcaag aacgggcgtt tcgctcagcg ggatgaagga ggtagggatg
     3601 acgcaggaaa tgatcttggg catggcggcc ttcgtggtca gtatcggtgc ggtgctgtat
     3661 gtgcgcctgc ggcgcccggc ctacgtcatg gtgaggcgat cgagccggga gcatcctcgt
     3721 gtccgctagt gccacgagcg gccatgtatg tcggagcgga atgccaacac accagcgaaa
     3781 ggtacggtgc ggacgattat tttttatcga agtcgggggt tccaatggac acacatggga
     3841 caccagaatc atttatgcct ccccagccgg gcaagcacgt ctgtccccgt tgcggagggt
     3901 tattgttgac gcagcattat attgatctcc tggatgatac aggccaaatc gatattacgg
     3961 cgtggcattg cgccatgtgc ggcgaagtgc tggatcccgt gatcctcaag aatcggcata
     4021 gtccgccacc caatttgctg tatggtacga aagagcgaaa gttcagtcag cgtgtgacgg
     4081 ctgtttccaa tggcggtgcg tcgaagaagt cgtcccatcg cgaggcggca ccggataaag
     4141 gaaaggactc ggagactggc gccgatcgcg ccccgtagcg gacgactgcc ggaactggag
     4201 gctcccacat gaatattgtc gtcaccggag ggacaggctt catcggccat gcgctcgtgg
     4261 aagaattgtc gcatgcgggt cacgacgtcg tggtgctgag ccgaaggcca cattccccca
     4321 ctcagtcgaa ggtacgctat gtggagtggg atgcgcggtc agtcggcccc tggcaggacg
     4381 cggtcgcggc ggcggatgcg gtggtcaatt tagcgggcga gccgattgcg gaaggccggt
     4441 ggacaagcgc ccgcaagcgg attcttttcg agagccgtat tctcgccacc agatcgctgg
     4501 tggatgcgat agcaaccagg tctcgaccgc tccctgtgct gataagcgcc tcgggcatcg
     4561 gctactacgg cctccaggat gatcgtgtcc tggatgagcg atcccccctc ggtgaaggat
     4621 ttcttgctca attatccgca gcctgggaag cggaggccct ccgggccggc cagttcggca
     4681 cccgtgtcgt gcttctacgg acggggatgg ttctggaacg ggatggcggg gcattgccga
     4741 aaatgatgct gccgttccgt ttgtttgcgg gtggtccggt cttgccgggg acgcagtggg
     4801 tgtcctggat tcaccgggcg gatctcatcg gcttgatgca atgggctcta cactccccgg
     4861 aggtttccgg ccccgtgaat gcggttgcgc ctgggccgga gacgatgaag gccttctgcg
     4921 gcatcctggg caaggtcttg caccggccat cgtggttgcc ggttcctggg ctggcattgc
     4981 gtctgggttt gggagagctc ggaacgttga tgacaaccgg acaacgcgtc gacccagcta
     5041 aggcacaagc agggggctac aggttccgat atccgacact ggaaccggct ttgcgcgcga
     5101 ttatgacaaa ggaacagcgg gcatgagcct cctcgttgaa tcgatcatgc agtatctgca
     5161 cggcctggct gcggcgatac tggtgggcaa ggtggtgctg ttatcgtttg tggtggctcc
     5221 cgttctggca aagcagttgg ccccggagca gttcgggaaa gtcgtgcggc aattgttccc
     5281 tgcgtactat ctcctcggaa tgggcgcggc tgccgggggt ttggtgtcgg tgaccgcact
     5341 cttggtcatt cgtggggcca gcccgggcct gtcggcagcg ggtggtatct ggctgatcat
     5401 cttcctggcg gaagcctatt gccggtcgcc actgacccca cagagcaatg cgatgagaga
     5461 tcagcttaag gaacaggaaa gtcgtggttc cgtggatcag gttctgaagg cctcttggga
     5521 gcgattgcat cagcggtcgc tctatttgaa ttcgctcgtc ctcctgggag ggctgtgctt
     5581 ggtagggttg gcctcttaac ggtcggtctg aagaagggag tgcatgatgc gcaaacacac
     5641 gtatagggtg gcatggatga ccctcggggt cttgagcctc tgtttgagtc agtggcccga
     5701 gggaaccggc aagtcttggg ccgcggaggg acatgagaag tccgggactc ccggagggtc
     5761 gtatggattg gaggtcacga ttccggacgg cgtcatcggc gtctcgttgc acgtcggnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg gattggaggt cacgattccg
     5941 gacggcgtca tcggcgtctc gttgcacgtc ggagcggagc gagtcggtga cacggcggtg
     6001 ttgtacatcg cacaggtgtt gccggatgga ccggcggaga aggccgggct caagtcgggg
     6061 gatgaaatta ccacggtgga cggtgcggtc gtcaccggca agacgtatga gcaggtggcc
     6121 ttgatggtga gaggcgaacc ggggacgatt gtcaggttgg gaatcaaagc agaaactgcc
     6181 gtgcgcgaag tggccgtgac gcgggtggcc ggtcagacac tgtacaaggg ccagatggga
     6241 tcacatggcg gctctgccaa ataggtgatg tgtgaaacgt atgatcgccc tgctcggatg
     6301 cttcgtcatg atctcgggag tatgccttac cgaggtgagg gcgaatccca cgccggcatc
     6361 gacgctatcg ggggtcgaaa aaacagggct gtatctgctg gccggtgact atcgtcgcgc
     6421 gctggagagt tgtgaacagg cgttacagga gcgcccgtca gtcgaggcct atgttcggtt
     6481 gacctatgtg taccaggcca ttgacgcgta tcttgaacag ctgtccaagg atgaaagctg
     6541 gatggcagtg gagcacctct atctgaacct ggcgtaccgg cataccgagg atctcgtcga
     6601 tccgcccggt ggtctcgcac gcatggcgaa ggaaatgatc caaaccagcg ttcgccaaca
     6661 gtctgacgtc agcgccgcga tggcggtgcg gttgaacaaa gtggcctccg atcgcctctg
     6721 gcaggagcag actcagtggc gacaggcgca tcccacgacc tggtggcagg cgttccccga
     6781 tgcctgggtg cagtgacgcg atggagcccg acggtatgac gtgtccaggg aaggaggttg
     6841 gaaccatgat ccagcagacg agtcaacgcg gcatcgatat caaattattc ggggcgctct
     6901 ttttgctggt gggcctgctg gatgtactca tcattgaatg gttccccgcc tatgcgctca
     6961 aactattcgg cgtgacgatt accggcccga tggcctatgt tgtgaagctg cattccccgg
     7021 ccgcccattt tttgatcggc tacggattta tctttctgcg tccctgggcg tggggattgg
     7081 ccatggccta cggggggttc ggcctcacca gcgaactgat gaatcaatgg caattcggtt
     7141 tccatcaact gcgcacgggg ttcatggcca cgacggccct gtttctctgc tatgtggcct
     7201 ggcgacgagt gctgtttgtt gatccgctgt cgcccgacac gcggcttgcg tcctcgtctc
     7261 gcgaggttca tttatgaggg ggttagtctg gtttcgtcgt gacttgaggc tgcacgacaa
     7321 ccctgctctt tcggctgctt gcaaggaatg caaagagatc gtgccacttt ttgtgttcga
     7381 cgacccattg ctggccagcc atgtgttcgg atccgtctgc gtggggttca tgttgggctg
     7441 tcttgaggat ttgcgccgct ccttggcgga acagggtgtg acacttgtct ggcgaatggg
     7501 tgagccgatt gacacggtgc tacgcacggt gcaggaatgt gccgtggatg ccgtctattg
     7561 gaatcgtgat tacgaaccgg cggcggtcgc gcgcgatcgt acggtgcaac agcgcctggc
     7621 tcagcagggc cgtctggtga ggaccttcaa agaccacgtg gtgttcgaag ccgaagaagt
     7681 gcgggggtta accggtgacc cctttcaacg gtatagcgcc tatcgtgatc gctggtggac
     7741 tcagtggcgg tctgctgcgc caccgctcct atccgtgccg tcattccccg cggcgtctac
     7801 agcgatgaag gtgtcggcgt ggcccacggt gcaggagttg ggttacgaat cggtaccgat
     7861 gtggatcgaa ccaggcgaac aggcggcgcg agccagactg cagtggtttt tgaaaaagcc
     7921 ggtccatagt tatgtcagtg gacgaaatct tccagcgcaa gacgggacgt cgaagctttc
     7981 tcctcatttg cgattcggaa cgatctcgac gcggacaatg gtgcatgccg cgttacagac
     8041 cctctccaaa ggcggtcagg tatcccgccc cgacgtgttc acctggatgg acgaattggt
     8101 ctggcgagaa ttttttcagc aagtgctgat ggcctttccc catgtcaccg aagggccgtt
     8161 caaggtcaag ccgggacttc ccgctcctcg tcctgccggt cctgagcgcg accggctctt
     8221 tgcggcctgg tgtgaaggcc gtaccggata tccgattgtg gatgccggca tgcgccaatt
     8281 gaatcagaca ggctggatgc ataatcgtgt gcgtatggtg gtggcgtcgt ttctggtcaa
     8341 ggaccttcgg atcgattggc aaagcggcga gcgatacttc atgcagcacc tggtggatgg
     8401 agatgtggcg gccaacaacg gaaactggca gtggtgtgcc tctaccggaa cggatgcgat
     8461 gcaggggtat cgcatcttta acccaacgct ccaaagtgaa aaattcgatc ctgatgggca
     8521 ttatctgcga tgctacgtgc cggaattgac ttccgtgccg acaaaatgga ttcatgaacc
     8581 gcagctcatg ccgcaagaag aacaaatccg cgtcggatgc cgtatcggat cggattatcc
     8641 ggagcctatc gtgaatcatc ggcaggcccg gcaggaatat ctggatctcg gcaaacagaa
     8701 ggtctcaacg tgacggccgc gacgctccgt ctcggcatca gccggtgctt gcttggtgag
     8761 gaggtccgct tcgacggcgg ccacaagcgg gatgccttct tgaccgacgt attaggccgt
     8821 tatgtggaat gggttccggt ctgtcctgag gttgaggcgg gactgggcac accgagagaa
     8881 gctatgcggt tgatcggcaa tccgcaacag ccgcggttgg tcacgctcaa gagcgggcag
     8941 gatcataccc gtgcgttgga ggtcatggcg gacaggcgcc tcgatgagtt ggccggcgcg
     9001 gacttatccg gctttgtgtt caaaaaagat tcgcccagtt gtggagtgga acgagttcgg
     9061 acctacaacg agcacggcat gccaggtcgc aagggcgtgg ggctatttgc gcgggcgttt
     9121 ctcggccgct ttccgctgat acccgtagaa gaagaaggac gcctctgtga tccgttgctc
     9181 cgtgagaatt ttatcgaacg ggtattctgc tatcggcgtt ggcgggacct gatgaacagc
     9241 ggtgcgacga ggcaggcagt ggtgcggttt cacaccatcc ataagtacct actgctggcg
     9301 catagcgcgc cgcactatcg ccagctgggc cggttggtgg cacaggtgag cgagtatcgc
     9361 cctaaggatc tggcgcttca atatggcgaa ctgttcatgc aggccctggc ggttcgagcg
     9421 acggtgcgca aacatgtgaa tgtgctgcat catatcctcg gacatttcaa ggaacggctt
     9481 gatgcgcaag aaaaggcgga gttactggga gtgatcggcg attaccatca aggactcacg
     9541 ccgctgatcg tccccctcac gctgatcaaa cactatgtgc agctcttcga tgtcgagtat
     9601 attcgcgagc aggtgtatct gaatccgcat ccgaaggagc tgatgctccg gaatcacgtc
     9661 tagaaaggac gtcatgccgc atgtcgtggt cgagggtgcc ggtgaccttc agagtctcta
     9721 tcagaacttc gccccgatgc tccaacgggc tgaccaggaa attctcaagg tccaggaatt
     9781 ctacctggcg aagagcggca gggaggcgct gctcgatgcg gtggtgatcg aacagggcgt
     9841 ggcacggagc ttttttattc agttgaaaca acatgacacc accatcaccg ttcggctgct
     9901 gcctgccacg gaccccgaaa aaactgccgc cgtgaaaagg gtgatggcgc tcgtggcgaa
     9961 atttattcgc acggtgtgtc cggcgagccg gtacggcaag accaatttgc aggaattctt
    10021 agaggccagt gttgcgaagt aagtatcggt ggatgtgaca ttgaatggag gactgacgaa
    10081 atggcaacag tgcggaaagg tgtgattctg gtgggacatg gcgggattcc cagagggtgt
    10141 cctcaagaca tggtgacgaa actcaaacga ctggaggcgc agcggcgtgc cgcgaagcaa
    10201 ccggcttcag ctgaagaaat ggagctggac gccaagatcc gtcaatggcc gaggaatgcg
    10261 gagaccgatc cttatcaggc aggactcgag gcggtggcgg cgcaattgcg cgcgaagctg
    10321 ggggatgtat tgtttgcggt ggcctataac gagttttgtg cgccgacgtt ggaagaatca
    10381 gtcgaacaac tcgcaaagca gggtgccacc cacattaccg tggcgaccac gatgtttacc
    10441 ccaggcggat ctcactcgga agtagagatc ccggaaattc tcgaacacct ccggcctcag
    10501 taccctggcg tcgagctccg gtatgcctgg ccgtttgacc ttggcatgat cgccgggacg
    10561 ctggctgaac aggtccgtcg attctcttga ggagaatcgc aatcggttga ggggtcttgt
    10621 gaacgcagct gatggacagg actgcggctg tcaggtcggt gaatcatttg atgccatctg
    10681 agtggagacc ggtgtacaat cagccgccgt tgcgaggcgc cggtactgct acggtggctt
    10741 gatgaatcgg ccttcaaggg agcgcaggac ctatcatgct gcaagacgaa cttatcgctg
    10801 cgtttcacaa gacgcagtcg ttcaagtggg atccgaaagg cggctttaag ctcgcctcgg
    10861 gattgctcag tccgttttat gtcgattgcc ggacgttaat ggcgttcccc cacgcccgcc
    10921 acttagtcgc caagaaagcc tgggaggtca tcaaggacct acagatcgat tgccttggcg
    10981 gactggaaat cggcgcgatc tcgatcgcca cgtccatctc ggattatgcc tattcggctg
    11041 ctcctcgacg cgagtggcgc accttttttg tgcggaagca ggcgaaggac catgggcttg
    11101 gtcgattagt cgaaggtgtc gtgcgaacgg gggaccgcgc cttgatcgtg gatgacgtgt
    11161 tgacgtccgg cggatctgtg gtgaaaacga ttgtcgcggc ccgtgaggct ggattggaag
    11221 tcacggacgc gctggtgatt gtcgatcgaa aagaacagga tggccgcgcc cgggtcgagc
    11281 agactggggt gaaagtcatg agtctgttga cgattgacga tctgatgaag ggtggcccga
    11341 ttcctctctg actatttgca ctggaactga atgccccacc gccgttcctc gaccacgtcg
    11401 cttgatcccg caacaggact ggcctgacgt aggcgaccct tcgtttcccc ttctcgaacg
    11461 atcgcatagc cgcccgggca tttctgctcc atcagacgca cggcctcttt tcggaacgac
    11521 gacaggaggg tcccctgttc tgctctgtag gggtacacga ccacgcctcc gtcggcactg
    11581 tccttcacca gaagggcgcc ctcgccgcag cttgatagaa acagcgagac agtaaggact
    11641 gtgcagcttg accacctttt catagggctt ttatcataca gcatatgtcg cacacttatt
    11701 ccggctcgcc aaaaggagag agataaccat gcgatacctg acgtctatca tgtgtgggct
    11761 ctgtctgtgt gggcttcttt ttctgctcga tgtaccccag ccggccgtcg cctatcactc
    11821 ttatgtgttg agcgtgcagc aattgcgggc cggtctcatg aaagcctcgt ctacgaatgc
    11881 caaggggttt gttctggtcg atgtgcgttc ccctgaagag catgcgtccg gcatgattcc
    11941 cggcaccgat ctgaacattg attttcgcga gatgaaagcg cgacatcgtg agctcggggc
    12001 gaagttggaa gaccatatcg tcgtctactg tcaatctggt catcgcagca atattgccgc
    12061 ggagactctg gccgatctgg gctatacgca tgtgtacaat gtggtcggaa gcatgaatgc
    12121 ctggaccgag gcaggatttc cagtctcccc tggccgatag atcagtgtgc gcgagtggac
    12181 tgtgacggcc cgtgagccgg tgtgctgcgc gaaggggtgc gtggtgtgat gccgatcacc
    12241 gtgggcagcg gaatgtgtac gcctgtcgtc ccagcaagaa gcgatcgcca tccggcctgc
    12301 gaggaggttt cctgggccgc gtgcaacagg tcggggctgg caatgacatc ggacgatgcg
    12361 ccgccatcca tggccatggc ctgctgaaga gccggcagtt ggcgatgtaa acagtcggcc
    12421 aggtggtgga gcgagacgat gtccactgtc ttgatcacaa gaatggctcc ctgcccgtct
    12481 tccgccacca cggtctgaaa cgcccgtttt ccgctatcct tgacccgaag cttgccagtc
    12541 cggtcgaata gcatcagcga ctgtgcggcc tctcgataca gtggtggatc ttcggtgaat
    12601 ccctcatagg cgagatccag cacgcgtgct tttttcagcg ttccgtcggt gggttcggcg
    12661 gcgaacaggc cttgccagga atggtgtttc ttggtgccga gcgaacgccc ctccttcaat
    12721 agcacgccca gatacgaata gtcctcccga aacaggcccg cattgaacaa gacatatgca
    12781 tcggtccgtt gctgccagtc gtgaatcgag attggcgacc gcaacccttc atcgcgaaat
    12841 tgatggatgg aaaaacggta ccgctccgga tcgatctgca gcatgagcag cgagggaacc
    12901 tgagggcagg cctccatggg gttccacaac gccacgtgca tgcctgcggt taatcgctcc
    12961 caggggactg gttctgccga agcggctgcc gtctgaaccc atccatgaca cagagacaga
    13021 aggcagacgc agaggggaat catcctgcga agggatcctg gtaagtatcg gtgcggattg
    13081 atcatcggag caggtggtct ttcatggtaa tcagccaacc atggcagcgt gaaacgtctc
    13141 gcgcgggctc agtataaaaa ggatgggggc aggggtcaat tccgtcggga atatcggtca
    13201 gatgagcacg tttctttagg aggcgcgagc ggtgcgcggc gcttggtccg gtggagagaa
    13261 cgggtgttga gtctggcccg ttgcgggaga ggacgagtcg agtaaggcac gcaccgtgcg
    13321 taacagaata tccggggtga acggtttttg taaaaacgtg gttcgatggg gatccatacc
    13381 gctgttgacg cagatatcgt cgagataccc ggacatgaac agcactcgca ggttcggctt
    13441 gatgacggag aggtgctgcg cgagctcgcg gccgttcatg cccggcatga ccacatcggt
    13501 tagcaagaga tcgacatgat agctttgctg cgtggcggtc aggcaggctt cgacgccatt
    13561 cttcgcttca atcacttgat acccggtttt gaccaattca tgacgaatca agtcgcgcac
    13621 gccgccgtca tcttcgacta gcaggatcgt ctccgatcct tcgcgtggcc ggctttgggg
    13681 tttctccgaa cgagcggcag aggtctgcgg atttgcgcgc gggaaataca agtcgaaggt
    13741 ggtgccgtgt ccgacctgac tccagacatc gatgccgccc ccgcaggtgg tgacgatccc
    13801 gaagacggtg gacagcccga ggcccgtgcc cttgtgttct tctttcgtcg taaaaaacgg
    13861 ttcgaataag tgggcgagga cctcggcatc catgccgcag ccggtgtcgg tgaccgtgag
    13921 tttgacgtag tgtcccaacg gcaagggatg gagatgatgc atgggcgtgc gcgccagttc
    13981 cgtttgcgat gtttcaatcg ccagcaggcc cccgttgggc attgcatccc gggcattcac
    14041 gaccaaattc atgagcactt gttccagttg ccccggatcg gccttgacat ggccgtgatg
    14101 cgggtcggga cggatgacca attgaatatc ttcgccaatg agacgacgga gcatgccctc
    14161 tacattggcc aggatggcat ttaatgggag cacctgcggt tctactggct gcttgcggct
    14221 gaaggccatg agttggcgaa ttaaactcgc ggctcgctct ccggctttct gcatctcttc
    14281 gatctttaca cgaattgggt tgccggcggg cagttccccg aggagcactt ggctgtgtcc
    14341 catgatgacc gtcaggaggt tgttgaagtc gtgagccagt cctcctgcaa ggcggccgac
    14401 ggcctccatt ttctgcgcct gccgaaactg tttttcgctc atgcgcaacg cgtcttcggc
    14461 ccgtctccgt tcaagacgct cgtcaagttc acgcagggtc cgtttgatgg atgggacaag
    14521 tctgcccagg cgttgtttaa agatatagtc cgtcgccccg cgctgcatca tatcgatggc
    14581 gaattcttcc ccctgcgttg cggagacgaa gaggaacggc acatccggat gcagggttcg
    14641 cgcgagaccg agcgcggcgg cgccgtcgaa accgggaacg gtggtgtcgg caagaatgag
    14701 gctgaagctt tcctggcgga gggctgcgag aaagtcttca cgcgtgtgaa cgcgcttcgg
    14761 ttggcacaga atacccgcct cccggagcgt ggagtcgatc agatccgcgt ccgccgggtt
    14821 gctctcaaga tggatgaatc gcaggggctg agtcatcatc tgcaacgaga tcggaattag
    14881 ctggaccggc cggcggaccg gctggctcct tcgggcggtg gttcgttgat catgccccag
    14941 aacacgccca gttctttgac ggctttcaga aactgatgaa actcaaccgg cttgacgaca
    15001 taggcgttgg ctccgagtgc ataactctca gccaaatcgc gttcttcgcg tgaggaggtg
    15061 agcatcacga cggggatcgg cctgagggcc gcatcgttct tgatcgtgcg caggacctca
    15121 aggccatcga ccttgggcat tttcaaatcg agaagcacga cggccggatt gccttggaga
    15181 cgcgttttga aatggccgcg gcaatagagg tagtccagca cttcggcgcc atcatggcac
    15241 aggatgacct tatcggccaa atgatgctct tccatcgcag ccagcgccag ttcggcatcg
    15301 cgcggattgt cttcagcgag caaaatgggt ttagaagcga tcatgccaag ggcctcactc
    15361 tcgggaggct gacgtaaaag gtggcacctt cgccgagcgc accttccgcc caggttttcc
    15421 ccccatgtcg atgaataatt cgcctgacat tggccaatcc aatgcctgtc ccttcgaact
    15481 catccgctcg atggagccgt tgaaacaccc caaagagctt gttggcgtaa cgcatatcga
    15541 atcctacccc attatcacgt acgaataaca ccgcttcatc cgggccgttg tccagactgc
    15601 cgatttcgat cgtggcctgt ggcctggtcc cggtaaattt aatcgcgttc gtcatcagat
    15661 tcatgaaaac ctgccgcaac atggcggcat ctcccgtgac ctcagggagt tgagcgattg
    15721 tccatgagat tgcgcgatct tgcaagtcat gtcgaagatc gtgccgcaat gcggcgatta
    15781 attggttcaa attgactgtc ccccgcaaca tttcctgccg ccccatgcgc gaaaacacca
    15841 ggagatcgtc aatgagttgt cccattcggg tggcggattc cgaaatcgtc tgtaagtagc
    15901 gtttcgcctt atcgtcgagt gagatagacg cggctttcgc gagcagcgag gcatagccgt
    15961 caatgtgccg gagtggcgcg cgcaagtcgt gcgacaccga atagctgaac gcctccagtt
    16021 ccttattggc ggcttcaaga agttctcccc ggcggtggag ttcgtcggca atccggcgcc
    16081 gttcggtaat atcgtgccgg atcaggtaat acacggccgc gaggagcacg aacagcaaga
    16141 gcgccccaat ggtcaacaga atgatgctgg agcgagtcgt ggttgttgat tcaacgaccc
    16201 gttgggtcaa ggctttcgtc tcctcctgat ccatctgcgc ttgcagtcgt ctcgctgaat
    16261 cgagtgcggt cttggcgacc cccgtgagcg ccattccccg caccgagtca aaccctgtct
    16321 tggcgcggag ttcgatggcg gcatgttctt cttggagctg ttgattgatc aaatgttcga
    16381 gatcactgac atgcccacgc tgtgtgctgc ctggatcggt gagctcccgc aaatagcgaa
    16441 tatattcggg cgcgcggtcc cgcaaggtgt tgtaggcggc cagataactc tcgtctcccg
    16501 tgacgagaaa tcgacggtgc ccgttctcag cgtcgtcaat cgcttctccg gttgctccga
    16561 gaaactggat gaactcgtga cttcgcttgt ctaaatcgcc attccgaatg aggaccgtca
    16621 tgttgcgata ggaaatggcg gaaatgacca ggatgccggc aaaaaccaac ccgaatccag
    16681 ccaggacgcg ctgttcgata gatcgcttct gaaacgtgtg aagcagtggc gagaggaacg
    16741 atactcggcc ggaacttttt tcttccgccg gcgaggcagg aggaaatgcg gagtcgagcc
    16801 cgacgtcaac gggttgatgg cggtttggtt cgttcatgcc tgcagtgaaa gctccgagat
    16861 ctggggactg gagcctggga attgcgcgtc aatgatgttg acgcagtcgg aagactgcac
    16921 agcacattgt accagaaggt actgatatgc gaagagaatt gatggaagcc gagaaaagcg
    16981 aagccggacg aatcctcagc ccccgaacgg ggtgaccggc ggagtcaggg aagcagcggg
    17041 tccaatgaga ttggaaaaca tcagcgtagc tgctacagcc caatcgagaa acggttgggg
    17101 gtagatgccg attacgagcg tcccggccag gccgacatag atcactgctc gcagcggacc
    17161 cgtggtcttg atgggagagg agtcgagcgg ttcgttgatg tacatcttct tgacgacgat
    17221 cagatagtag tacatcgaga caacgatgtt gacgaggccg acggtaatga gaatgtagag
    17281 gccttctttg atggcggcga caaagatata gagtttcccg ataaagccgg cgagtggcgg
    17341 aacccctgcg agtgagagca ggaaaattaa catggcaaac gccagaaagg gcgagcgacg
    17401 gttgagtccg cgataatcgt cgatctcatc actgcccacg gagttgctga tcgccatgat
    17461 gaccgcaaag gcgccgaggt ttgcgaagag ataggtcaac aggtagaaca agattgagtc
    17521 agtgcccatc ttggttcctg cggccagacc gatcaggaca tttccgacct gcgcgatacc
    17581 ggaataggcc agcaggcgct taatgtttcg ttgggcgatt gccacgatat tgccgtacgt
    17641 catcgataga atagatgcgg cgacgatgag caaggcccac gcgggcttaa acgtggccag
    17701 cgcgacaagg aacaaccgaa gcaggatggc gaaggccgcg actttcgggg cgatggacag
    17761 gaacgccgtc acaggggtcg gtgcgccgtg atacgtatcg gggatccagg aatggaaggg
    17821 aacggcgccg attttgaatc ctaatgccgc aaagatcagc aggaagccga tgactaatcc
    17881 cgtcgtcggg ttcgcgccac tcatgtcgga gaagacgagc ttgccggttt cgccgtagac
    17941 gaggctgatg ccgtacgcca gcaatcctgc cgcaaagact ccgaggataa agaatttgag
    18001 gccggcctcg ttcgacgcca tgtcgtctcg cagataggag accagcacgt aaaagccgaa
    18061 ggtcgcaaac tcgagggtga cgaagagcga taagagatca ttggccgagg ccatgaacat
    18121 catgcctaac gccgacatcg cgacgaggaa ataatattcg cctcgaaaga gcgagaagcg
    18181 atgcacatag tcgatcgaaa gcagaatgac caggatcgtg gccaggagga tcatgacttt
    18241 gaagaagatc gccatgcgat cgacgacgaa catcttcccg aacagggtgc cggtgatgcc
    18301 ggtcatgtcg aaccatgcga ggcaggccag ggtgataatc actcccaaga tggtcaggta
    18361 ggccaattgt tcctggacga tgcgtttgaa cgaaaaatcc acggccagga cgacgcacag
    18421 ccagagcgtg agaaacagtt cgggaagcag gaggagaaga tcgctggctg aaagcgtcag
    18481 ggagaaagtc atcgcgaggt taccttcgca atcgcctcac cgggaacagg cacgggcatg
    18541 gtgtgcgagc tggcttgtgg aatgttcatc ggaagctctg cgaccgggac cactttggta
    18601 atacgcgcga tcagcgggtc gacccctgat cggaccacgg agtagagatg tcccgggaaa
    18661 atgccgaagc tgatgctcac cgtaatcata atgagcaggg gcatgcgatc gaccactgcc
    18721 acggcgtcgt gcgagtggct atacttttcg gccatcgaac cgtagaagag gccgcgcatc
    18781 atcttgaaga ggtaggccat ggtcaacacg atgccgagca ctgccacgac gacttggaaa
    18841 gggtatttat tccagctgcc gacgatgatc atcacttcag cgataaagtt gacggtcccg
    18901 ggcattccga tcgaggccat gcaaccgatg acgaaggccg cagagataaa cggcatccgg
    18961 ttggagagcc ctcccaggga cgggatgtct cgagtgtgtg tttgatcgta gacccagccg
    19021 gccatggcga acagcatgcc ggtggccatg gcatgcgcga acatgtagat gacggctccg
    19081 ctgaggctga tgtagtccaa ggccgccatg cccaagaaga catagcccat atgacttgag
    19141 cttgaataac cgatgacgta tttcgtgtcc ttcgcatagt acgccaccag cccgccataa
    19201 ataatgctga agatacagag caccgcggcg atgggcatca gttctcgggt ggtctctgga
    19261 agaatttcga acgccacgcg aatgatcgaa aagtggccga gtttcatcag cacgccggca
    19321 tgcaacatgc tggtagcggc cggggcggcg gcgtgaccta ccggagacca ggaatgcaat
    19381 ggccagatcg gcgcgatgga cgcaaagccg aagaaaatca ggagccagat gagcgtggcc
    19441 aacggccctg agaaatgggc tttctcgcgc aagatcagga tatcgaacgt gttcagtccg
    19501 gaaaatttgt agatgagcag aatccccatc aaggcggcca cggcaaaggc ggaaagaaac
    19561 aggaccagct tcatcgccgc atattctttg ctgtttgcgc cgaagttgaa gataaagccg
    19621 acggagtcac gttgtttcag gccttcgggg tccgtcattt caaggtattt cttggtatga
    19681 ctgccccaca tgcccaaaag taggtacatg gggatgacgg acatttcata gaagaagtac
    19741 aggaagaata agtcgagcga catgaagacg ccgatggtcg cggcagcgag aatcaggatc
    19801 cagatgtaga actctttcat ccggtctttg atatgccagg agacgaagat gccggcgaac
    19861 aacaaaatgc ctgaggcaag caccagcgga gtgccaatgc catcgacgcc gagatgcagg
    19921 gaaatgccga gctgtcggga ccactcaatc cgctggatga actgatagcc gcccttcact
    19981 gaatcatagg cataaaagag atagacggag gcggccaacg agacaaacgc cgaaccggcg
    20041 gcaatgcctc gtaccagcga aacttgacgg ttggagacga aaattaatcc cagggctccc
    20101 agaaagggcg cgaacaaaat gatgagcagc gtgtattcag ccatatctta cttcgctggc
    20161 acgtggataa cagtgtctac tgaggccact ttcgcacgat caagtcggtc gacgagtgct
    20221 tgaatggatc cattcatcat tctgaggaag ggcgagggat agaggccgat ccacaaaatc
    20281 ataatggaga gcgagagggc aatggtgatc tcacgcatgt ggagatcctt gacggccgcc
    20341 gacacgtgtt tgctgagcgg gccgagcatg gctcgctcgt aataccacag aaaatacgca
    20401 gctccaaaga tcacgccgag tacggccacg gccccgaacc accatttcgc tttgaaggca
    20461 cccaaaagaa tcaagaattc cccgacgaat ccgttggtgc caggcagtcc gatcgacgcc
    20521 atgccgatca aaagaaggaa ggtcgccagg agcggagcct gcttggcaaa acctccgaag
    20581 gacgataagt gcgagctctg ttggcgggtt gacaggaacc cggccatgaa aaagaggccg
    20641 gccgtgctga accccagatt gatcatggtc aacaggctgc cctggagtcc ctggaaattc
    20701 aacgcaaaga ggcccaccac cacaaatccg aggtgactga tactgctaaa tgcgagtaat
    20761 cgtttgaaat ctgattgcac cagcgccacg accgccccat agagaatggc agacagggct
    20821 aataccatga cgatgccaac cactccttcg ctttttgatg catcgggcac taatgggaag
    20881 gtgaagcgga ggaatccata ggtgcccaat ttcatgccgg ccaacatgac ggccatgccg
    20941 atcggacctt cgacgagggc atcaggaagc caggtgtgaa atgggaacac cggcgctttg
    21001 aacgcaaacc ccataaacat gagccagaag atcaaaatct gctgcgagag tggaatggga
    21061 acggagagca gctcaagcag gtcgaatgaa tacacgtgat ccatgtgatg actcacggcc
    21121 cactggtgat agttaatgtc gagcaaggca atgccgacca acataaagac gctgcccaga
    21181 agtgtataga gaacgtattt caaagctgcg taatggcggt cggctcctcc cccccacagc
    21241 ttgatcagga agtagctggg aatgagcatg agttcccaga acacgaagaa gaggattaga
    21301 tcgatagaga caaaaatgcc gatcgtggtg gtttcaaggg ccaggagggc catgaaatag
    21361 aggcgcacct gattacggat cgtgtcccag gaatagatca ctaccaagac cgtcagaaag
    21421 gccgtcaacc ccacgaagag cacgctgatg ccgtcgacgg cgaggtgata tccgattccc
    21481 agcgccggca tccaccgggc gtgttcggcg aactgcatgg ccgccgaatc agggacaaaa
    21541 cgcacgagga cgaggcaggc gagcgccagt tcgacgaggg taatacccaa tgtcgtcgat
    21601 ttgagaagat cctcatcgtc aatcagccag agaaccgccg ccccgaccag ggggaggaag
    21661 atcaggtacg agaggatggg gaacccgaaa ctgaactctt caagcatcgt aaatccaaaa
    21721 atctgccgcg tcagcgtgct gaataacctg tcgaaccgcc tccggtccag atcaacaaga
    21781 tgaggtggac gagaatgaat agtcccgcca cgatgatagc tgcatactga tgtaccatgc
    21841 cggtttgcag gcggcgccag gagcgtgcca agaggtgatt cgcatagccg ataatattga
    21901 ggccggcata gaccacgtgt ttttccgtcc aggtaatcgc cgcagagctg aggtcggtgc
    21961 ctcggtcaat gccgcggaca agtccgtcaa taatggtgcg gtcgaaccaa tcgcagaggc
    22021 ggcccaattg tttggtgggt tccacgaaca gggtgtcgta gagctcatcg acatagtact
    22081 tgttgagcaa ggtggtatat gcgccgggca tgcggtcggc aagggctgcg gcggcggttg
    22141 atgggccgct atagaagtag tgtgcgaggc cccatccgag cagcgcgatg acggtcgccg
    22201 tccccatgag cccaaacatc aatgctggcg gcgtcgcatg ctcctcaccc gcgactcctg
    22261 ccacggaatg gaggaaatgg tgaaaccagc cgttttccgg tggaacgcca ggaaacccgc
    22321 cgatgacgga caacgtggcc agcacgacga gagggccgat catgaccatg ggcgattcgt
    22381 gcacgtgttc ggccgtatga tgatcgagtc gcgacgttcc ataaaatgtc agatacgtca
    22441 ggcggaacat gtaaaacgac gtgaggaagg cgccgactgc tgccatgccg tacaggagat
    22501 agtgatgatg gacgaaggca tggcccatga tttcgtcttt gctccagaac cccgccaaag
    22561 gcggaattcc cgcgatcgcg atcgtgccga tcaggaacaa tgtatgggtc catggaattt
    22621 tcgacttcaa ggcgcccatc ttgcggatat cctgctcgcc tgaaagagca tggatgaccg
    22681 aaccggctga caaaaagagg agggctttaa agaaggcgtg ggtcataaga tggaatacgg
    22741 cggctgtgta ggcgccgaga ccgcaaccaa ggaacatgta accgagttgg ctcacagtgg
    22801 aataggccag gacacgcttg atgtcggtct gcactaggcc gatggtggcg gcgaaaagag
    22861 ccgtcaatcc gccgatccag gcgacggtcg tcatcgccat cggcgccagg tcgaaaatgg
    22921 cgtgattccg aacgatcatg tacacgccgg ccgtcaccat cgtggcggca tggatgagcg
    22981 cgctgacagg ggtgggacct tccattgcat cgggcagcca ggtgtagagt ggcaattggg
    23041 ctgatttgcc gacggctccc acgaggaggc agagcgcgat cgccgtggcc atttcaggcg
    23101 acagctgcgc cgcgttttgc atcacctgcg tgtaatcgag tgtccggaag ttcacgaaga
    23161 caaggaaaat ggccagcaaa aagcctgcat cgccgatgcg attgaccaca aacgccttgg
    23221 tcgccgcttt ggccgccgat accttgtcgt agtagtagcc gatcaacaga tacgaacaga
    23281 ggccgacgcc ttcccacccg atgaacagga ccacatagtt gttgcccatg accagcagga
    23341 gcatcgacac catgaagagg ttcatgtaca cgaaaaaccg tgtgaagccg gcttctccat
    23401 gcatgtaccc cacggagtac acatggatga ggaatcctac tccggtgatg acgagcagca
    23461 tgatgcaggt tagggggtca atcagatagg cgagattaat cgtgagatcg ccgccgaaga
    23521 tccatttata ggcaatgact tcgcgagccg ctccggtacg taaggtttcc gtgaagactg
    23581 ccagcacgca gaggaacgac atgaggaccg aaccccatgc aaagcggtga gccaggtcgt
    23641 gagaataacg atgcccgaag agaccgttca caataacggc catcagcgga aacaggggaa
    23701 tcagttttac gatcaggtca atggagtcgg acacgttacc acttcaatag gttcatttcg
    23761 tcgacgttcg tcgagatctt gccgcggaat acgacaataa taatggcgag tccgatggcg
    23821 gcctctcccg ctgcgatggc gatgataaac aacgccacga tctgaccttg catggattcg
    23881 aggtagtgag aaaaggcgac caggttgatc gtggccgcat tcagcataat ttcgacggac
    23941 atcagcacga taataaagtt gcggcggatg agaaccccga gcaaacccgt cgtaaagaga
    24001 atggcactga cggcgacgta ggcggataaa ggaatcatgc gtcccgcctt gtttcgatag
    24061 atttgggcgc cttcgccaga acgatcgcgc cgacgatcgc gcctgtcagg aacacgccga
    24121 taatttcgaa ctgaagcaaa taatcgctga acatcttgat gcctaccgca tgcgcatctc
    24181 cgttcaacag aacggctgcc gtagatgcat cacctcgaga tccgctaaac ggggaacgga
    24241 tgagtaggaa cagcaggtag atcaacccga tgacagccgg agccaggaga tacggagttc
    24301 tcgcatggaa atagcggtcg tcagttttga ggttcaatag catgagcaca aagaggtaga
    24361 gaaccagaat ggcgcctgcg tagacgatga cttgaacggc ccacaggaac tcggcgttca
    24421 gcatcacaaa cagccctgat acatggagca acagagccag aagcgccaaa ccgcattgca
    24481 caggattctt caacgccacg gtgagaatgc cggcgacaat actgaccact gcgaaatacg
    24541 cgaaaaacac gaaagccata acggtacgat tcagctcaaa tgtttgggtt gcgactgtgt
    24601 gggctttagc acggactgcg gaaagtagta acgatactcc cggctctcgt ccgaattctt
    24661 ttcttgatta tgggtataca aatatttttt agcgtcctcg aggtggcgtt caccaacctc
    24721 gtagagccgt ttcttatcga agaggagggt ccgcttgtcg tgggtggaat attcatacat
    24781 tttcgtcatc gccagtgcgt tgaccggaca ggcttcgacg cagtatccgc aaaagacgca
    24841 cttggtgatg tcgatataaa actcactggc aaatcgctgc agcggtcgtt cgggatcttc
    24901 ctggctgatc accttgatgc agcgagaggg acaggccgct tcacagagat cgcatccgac
    24961 gcagcgatca cgtccgtcgc tatagcgaag gaggccaaga gcaccccgat ggccgtccgg
    25021 aatggtgcgc tgctcacgcg gatactggaa cgtaataggg cgatggaaca tgtgcttgaa
    25081 ggtgaccttc atggcgtccc agatttcata gaacaacgcc gcctgcagaa cttttttggt
    25141 caacgccccg acactcattc ccaccacctt tgacgacatc aaaaagacgg acgagcttac
    25201 tcttacgcac caaccttttc aatctttaca cgcgttgatt tgaaatacgg cacgccggtt
    25261 acagcatcga cttcagtcgt catcaaatct tttattggag gctcattaaa atgttcgggg
    25321 aaaaagcacg atccggattg gacatccggg tctgctttca ctccggcctg cacggatgct
    25381 tgctgcgagc tcaggcggac agtggattgc tcggtcaggc cgagtttctc cacatcagcc
    25441 ggattcattc gtatacgccc attattgggc tcaatattaa tgagccctga cgcctgcgtg
    25501 gacattttcc cggaatggta gaggacctgt cccatcacga gggtaaatgg ccgtgaggtg
    25561 tcgtccatgg cctgccgtct gcgataccgg gtgacaacct cgctggaata gccattagag
    25621 aggtatgcgt cagcggaggg agcaatacgg cgtggttgcc ccaagttgta gtagccaggg
    25681 agcagcttca taatttcgtt ctgcacatcc tgcggtgatt gatagggcca ttcgtagccg
    25741 aggccattgg ctaaggccgt catgatatgc caatccggca agctttctcc gacctggtca
    25801 agcgcctggc ggacacgcag aacttttcct tccagattgg tgaatgttcc gtctttttcc
    25861 gcataggtag ccgcggggag gacgacatga gccatgcggc ctgtttccgt gagaaacggg
    25921 tcctgaacaa tcaggagttg caacttgtcg agggccccct tcacatccat ggaggcgggc
    25981 aaggttgcca acgggttttc cccaatgaca tacagtgcct tgatttggcc tttctgaatg
    26041 cgggtgaaaa tctccagaag attggcgccc gtgccgatcg ccggaagggt cacttcccaa
    26101 gccttcccga aacgttctcg agccccggga tcgtcgaagc gggcctgccc cggaaggaac
    26161 tctggagcaa cacccatgtc gatggcaccc tgttcgttca cctcttcggt gacggtagtg
    26221 acaccgcatc ctggttgtcc caatttgccg gtcacccatg ccaggtcaac gagcttgagg
    26281 acattctcgt atccgttggg acggcggaca atcccttccg cgcacaaagc cacggcccgc
    26341 ggcgcttccg caaaaatggc agccacctcg cggatctggt ccactgagat cccggtcgtc
    26401 cgggatacct cgtccaggga tatttgagcg actgcttgtt tcaaggctgc aaatgcctgc
    26461 gggtgttgtg tcgtactggt ttcatcgacc agatcctgct cgatgacggc tttgacgaga
    26521 ccatcaatga gcagtgcttc ggtacccggc ttcaccagga gtggatgcga tgccaattgg
    26581 gcgatattcg tgttcgatga atccacgacg atcacttggg cgtgatacac acggatggct
    26641 tctttaatgc ggaccgcggt cagggggttg gtttctgtaa tattggaacc gatgacgagg
    26701 acggccttgg ccttggtcag atcttcccaa tcattgagcg gtcggccaat cccgacggca
    26761 tgtcggacgg cgtgcacgta gtttaagtgg ccgtatcggg cgctactgtc gagttgattg
    26821 gtcctgaggc cgccacgcat gagcttctga aacaagtaga gttcttcgtt ggtgcagcgg
    26881 gcagtaatga gtccggcgat cgattccggg ccgtgcttgc ggtggatgtc tgacaactga
    26941 tcgatgacgg tctgcatcgc atcgagccat ggcgtctctt tcagagtgtc gccggtgcga
    27001 accaggggtt gccggagtcg ttgctgactg tcgatgtatt gaaatccgaa tcggccccga
    27061 acgcacagtc cgccgtgccc cttggcggtt tcagaccggt cgccccattt gtttttccag
    27121 gaaagcggcg acgtcacgcg aacgacttcc gtgtcctttg tttcgaggtg catttggcaa
    27181 ccatcgccac aatagttaca cgtcgtcgtg gtctttctca tctgccacgg cttgtagaga
    27241 tatttcgagt acttattcgt gatggcgccg acgggacaca cggccaagca atccccgcag
    27301 aattcacagg cgagtggctg gtctcctttg gcgacaacct ggttgaaacc gcccttcttc
    27361 atgaattgca gcgcgtcgat catcaacacg tctttgcaga cgttgatgca ttccgcacag
    27421 gcgatgcagc ggttcatgtt gaagtccagc accaggctac gggtgtcttc tggaatgaac
    27481 ttttgtttgg cgttggccag gttcgtgacg ccatgctgga atgccatgtc ctggagttcg
    27541 cagtgcccgt ccgcgtcaca gacgggacaa tccagcgggt gaactgaaag atgtttctca
    27601 accgctttct ttcgggcggc gaagaggtca tcaccgtcgg tccggatcac catgccggct
    27661 gccgctttcg ccgtgcagga tcgtaccggc gccttcttcc cttcctgcat gacaaggcac
    27721 atgccgcacg atccgaacgg atcaaacgtg tagtggtagc acatggccgg aataatcttg
    27781 ccggtgctgg cgatcacatc gtaaagcgaa acgccgtcct tggccgatac cgttcgtccg
    27841 tcaatcgaca gttcaatggt cgcggcttca acgtccggat tagtcgctgg tttcaaaccc
    27901 atgctttggc ctagtactta gttataagtg tttggagggg actgagaagt tcacacttct
    27961 ttcacccacc tctcaatgtg cttatctgtc acactctccc atgacgatgt cgtatgttcc
    28021 gaaaatcgtc acggcgtcgg agatcatata gcctttcgac atatgatcga atgcccccat
    28081 atgaataaac gagggggcgc ggattttcag tcggtacggc ttcccgccgc ccgtgctgac
    28141 gatgtagaaa cccaactcac ccttatgcgc ttcggtcccg cagtagattt ctccgggagg
    28201 tgcattgaac ccctgcgaga acagcttgaa ttgctgaatc atggattcca ggttcgtgaa
    28261 cacgcgctct ttgggaggca gcgtcacgct gggtacatcc gccataatgg gcccatcctg
    28321 gatctgttcg agacactggc ggatgatctt gacgctctcg tagagctctt gcacgcggat
    28381 ccagtaccga tcatacgtgt cgccgttctt gccgacaggg acgctgaatt cacacttggg
    28441 ataggcagaa tagggttcgt atttgcgaag gtcgaaatca acgccggacc ctctcaacgt
    28501 tggtccgctc aagccaaagc tcaatgcatc ttcagctgaa atcacggcga cgcccttcgt
    28561 gcgtgccagc cagatacgat tcttctcaag gaaaatgacg tattcatcga tcttcggtgg
    28621 gaagtaatcg aggaattgct tcaatttggc aaacagagac ggggtgaagt cgcgctcgac
    28681 accgccgatg cggtaccagc tggtcgtcag gcgggcacca cagagctcat cgaaccaatc
    28741 caacagaatt tcccgatcac ggaagcagta aaaaaatacg gtcatcgccc cgatatcaag
    28801 ggcttgcgtg ccaagccaga actggtgacc gatgatacgt tgaatctccg cgacgatcgt
    28861 acgcaggtac tcagcacgat ccggcacggt gatgttcatc aatttttcaa ccgcgcggca
    28921 gtaggcgaag ttgttgtaca tcgcgcagac atagtcgagc cgatcggtat gagggatgaa
    28981 ttggtgatac gtcccttctt cggcgagctt ttccacgcca cggtggagaa aacccataac
    29041 cggagtcgac ttgaccaacc gctccccttc caactccaga atcaccttca acactccatg
    29101 ggtgctgggg tgttgagggc ccatattcag gaggagttcc tcggtcctga gcgttggaag
    29161 cgtttcgctc tccggatgct cgggatcaat tttataaacg gtagttcttt gatcttcgaa
    29221 ctgtgccatg gtcgttcagt ggggctaaac ctgttgccta tcgggtcggt tcatccaaaa
    29281 actcaaacgt gtctcgccaa cccttgccac gaagcggaaa atctttacgc aacggatatc
    29341 cctcgtcgta ttcgtccggc atcagaatgc gtcgaagatc cggatggtta cggaaccgga
    29401 tgcccatcat gtcatagact tcgcgttcca taaaatcggc gcctttccag acgtcggtca
    29461 gggaatcgac gatgcaatcg gattccggta cgcgagtttt cagacgaatc cgttgtcgtg
    29521 tacgaatcga gtagacctcg tagacgactt caaaccgatc ttcatcgtcg ggccagtcta
    29581 ctgagctgac atggacgatg tagtcaaacc gtaatgcagg atcgtcccgc agaaacttgg
    29641 ctacatcgtg caacgtctca ggcttcaccg tcacggcgag gtccccgcgc cactccacta
    29701 cttttgtgca agcgccagga aaggtgtcct cgatacgttg ggctagttgg ctcatgaagt
    29761 cgttccgtcc gtcagacctt cagcgcgtcc ttcacttctt tgggctggga gaggaagacg
    29821 cgcttctgca tgatccgctc ctgcaacttc agaatgccat cgaagagcgc ttctggggtt
    29881 ggagggcatc ccggcacata aatgtctacc ggcacgaatc ggtcaactcc ctgaaccaca
    29941 ctgtaactgt cgtatatatt tcctgacgtt gcgcaggagc ccatggcgat cacgtacttc
    30001 ggttcgggca tctggtcata aattttgcga atgacgggcg ccatgcgccg gcagacggta
    30061 cctgcgacga tcatcaggtc ggattgtcgg ggcgaggccc ggaagacccc tgcgccataa
    30121 cgatccatgt catagcgtga tgaaacagcc gcgatcattt cgattgcgca gcaggccaga
    30181 ccgaaggtca tcggccataa ggatcctttt ctggcccaat tcacggcttt ttcaaccgtg
    30241 agggtgatga catcgggaga gccgtcttta tcgtggccac cgagttgaat taatcccatt
    30301 ccaatgctcc ttttcgccag gcgtagacat aggcgacgag gaaaagcccg ataaagatca
    30361 acatctcgat caagccaatg aggccgatct tattaaacac aatggcccag gggtaaagaa
    30421 agataacttc cacgtcgaaa atcacgaaca acatcgcgaa gatatagtaa cgcacgggaa
    30481 acggcattct ggcgtcagaa aagggttcag atccgcactc ataggtggat agcttctctg
    30541 gttcaggata cttcggttga acaaaatagc tgataatcag cgtgccggcg ccaaacgcca
    30601 gtgcgatgaa cacaaagacg agtaccggga aatatcgact gagatactct aagaggagct
    30661 cggaaccggc cattagctcg aacctcaacg ggtttgaggg cttataaggc tatttaagag
    30721 gtcggaatct tagcccttgc tcatttttaa tagcaagcgc acaaaggacc tagggcttta
    30781 gtcccgatag tcctagaaag tgaatactgt ccaggagtta ggagcgcgcg tcgattgtca
    30841 cgcttcgcgg ggcttgcttg atgtcttctt gcgcgatgtc gctgaaacat cgctgatcga
    30901 atgctcttgc gtggaggcgg tcttcatctg gctggccgga gttctcaaac cagccggagg
    30961 agatccggcg ccgatatccc actcagatgc catgagcgtc gatggttgaa ccttggttcg
    31021 gcggggcatc tgagtgttga tgcgtcgttt gtccattgcg ttggtcaccc gctcgatagt
    31081 ggttccggga cgtattcggt tccgatctca cgataacatc cagagtggcc tgattcaaac
    31141 ccttggcctg tgcgcggttt cttgacaacc ctctgctctt tgctaaggtt gctacaggca
    31201 accctcctag gtgtgatcaa tatgaggaca ttatctatgc gcgccagcct gaccatcctc
    31261 tcatgcagtc tcatggctgt aacggccatc ccagtgtacg ctcagaatgc gcaaagcgtg
    31321 cgactgggtg aggcagcctt gacctatgcc gccgggtcga tcaggcaaga catgccgatc
    31381 gagggtgtca tcaatgtcat tactggcgac aatcaattgt ccgggaatcg aatgatgttg
    31441 ggctggtccg ggacggacac gctgtatctc aaactgaaaa tcccaggcga cgcggcgctc
    31501 ggtgacctct acaccgtata tcggcggtct cgcaaggtgt tccatcccat caccaagcag
    31561 tatctgggat atgtcatcaa tcgggtcggt gttgtgaaag tcgtgcagct ggatccggtg
    31621 ctcgtgggcg tgcaggttgt tcggtcttac gcaccacttt cacccggtga tcccgtcatg
    31681 cgctttacgc ctccggccgc ggaggaagtc agcgagcctg tttcttcagg gccagatgtg
    31741 gaagggatga tcgttgagtt acaggctgac aagcatatgt cactcgtgtc gcagggcaat
    31801 gtggtgtacc tcgacaaggg gcgcgacgat ggtctccggt ccggagacac tatggaggtg
    31861 tttcgcacgg gcggcggctt gcccgagcga aagatcggcg aagtgagagt gcttgccacg
    31921 gaacctcata cggccaccgc ggtcttgtcc aaggcttcgg cccgcgccct cgtcggagat
    31981 cgtcttcgtt ccaaacatgg cgtgcccgtc gaggccatgc agcctgagag cggagagatt
    32041 gatcgtcagg cagcaaccgt acagcctgtg atggtggcga agacggatgc ttccgtccgt
    32101 gaggcgaatg gaggccgtct gcccgccaca gggaaaatcg aacgtactcg tggaagtcgg
    32161 cgactcaacc tggacgatct ggccgaccag ttggaatacg agtccgggga agtcaaagtg
    32221 aagcccgccg gggtacccat ccttgaaaaa ttgactgagt atttgaccac tacggccacc
    32281 ggtcagcagg ttcgcgttga aggtcattcc gataacatgg aaatcggccc gtccttgaaa
    32341 ggcgtctttc ccaccaattg ggaattatcg aaggcccggg cggcagagat cgtccggtat
    32401 ttggtcgaga agggtggaat ggattctgcg aagctgtctg ccgtcggata tggagccagc
    32461 cggccggtgg cgagcaacgg gaccgaagag ggacgcaaga gaaaccgccg cattgaagtc
    32521 gtactggagt ccctcgaaga ggcggcccca gcgcaaccgg tgaaagcctc ggttgatgaa
    32581 acggcatcgg tgtcgacaca gtttacgtat aaccagatga atgccgtgcc ggtgaatccg
    32641 acgagcgtcc cggtgtcgga ggtgccgtct ggaccggctc cctcggatgt gacacccgag
    32701 cgtggcccga tagcccctga ggccggagtg ccggcttctc ccgctgatgg gatgccaccg
    32761 tcgcctgccc ctggctccta gtagaggcgg cggctggtct ctcgcaagag gccggccgcc
    32821 gttcagtgcc ctctctgtaa cctttctcct tatctgatct cattacctgt tcgggctcat
    32881 tcctcgcgcg agctgatccc ggttgtcatt gttgcaccgg gccggtgttg ttagaatgcc
    32941 gcatgctcga ccctcctttg cagcagccaa gtgcgatgat tccggctcct tctcctgcgt
    33001 atgttgatgt cgtattgcct cgtcggcttc atcgaccgtt cacctatatc atcccctcag
    33061 aactcaaggg ccgcgtcgcc atcggacgtt cggtggtggt gccatttggc tcgcaagacc
    33121 ttcacggcct tgtcattgcg gtgcatcatc ggcttccacc tggcgcccca cagaagggcc
    33181 tcaaagctat tcgttcgttg agtgcggcct cagcggatca tctgcttacg gccggccaaa
    33241 tcgggctcag ccactgggtt gcggaacggt atgccgcacc gtgggggcaa tgtatcaagc
    33301 tcgttctgcc ccctgtcgat cgagtggggg cgtcccagcc gcgatatatg gcgacagcgc
    33361 agggccttgc gacgaacgcg tccgtcgagc cgttgggaga aaccgaaaca gagttgctcg
    33421 cccgcttacg ccggcgtccc aagggcatca tggcgacgac tcttctgaag ggcgacaggt
    33481 cccgaacgac ccttgccttg caggcactga tccaaaaggg gctggtgagt cgttgtgaag
    33541 aaccggccgc ccagaaaagc gtccgtcctg caaaacaaca ggggcaggtg tcaggaggcg
    33601 gacttcctat ggagtcggtg gaggctgttg agaatttcga gctgcctcat gcggaagcca
    33661 tgtcctggcc aacggttctg gaacaggcct tggcaaagaa ggtcttcgtc tccttgctcc
    33721 tggagggtag ccactgcacc agacagtggt gtctgacgca agcggttcga gccacccttc
    33781 gcctcgggag gcaggcgttg attctcacgg gtgatgtcga gcatgcgagt cgtttagctg
    33841 attccttcgc cgcagaaggc gaacagccga tgctcctgca cagtggtcta tcgatgacag
    33901 cgcgggcggc ggtctggcag gcggcgcaag aggggtcggt caggattctg attggcaccc
    33961 gcatggcggt cttcgctccc ctgaggcggc taggccttgt gtgggtggat ggtgaggatg
    34021 atgcgtcttt caaagaagag caggtgcccc ggtatcatgc ccgagaggtg gctcactatc
    34081 gggcttcccg cgacggcgcg gtgctcgtgc ttgcctcgaa tcatccatct ctcgagagtc
    34141 tgtcggcggt tcaggaagga cgcatgacgg catgtaagta tcgcgatgcg ggcgaactgc
    34201 cggctgtgca cgtcatcgac atgaaggagt atcccagaga cgcctcagga gggacggtgt
    34261 tatcgcctcc tcttaccgaa ggactacgtg aggcgttgcg gcagcacgcc ttatcgatcc
    34321 tgtacctgaa tcgcaaaggg tttgcgagtg tgctgcattg cggggattgc ggggcgatgc
    34381 cgcagtgtga tgcctgtagt gtcgcgctga cgttcttcag gcgaagcaat catgtccggt
    34441 gccactactg cggtcgaacc aaacctgtgc ccgaacactg ttcccgctgt cagtcgctca
    34501 agttggaacc ggtgggatca gggacggaac gtgtcgaaga ggccgtccga cgtctgttcc
    34561 cgcaggcccg ggtcggacgt gtggatggcg aaacgattcg tcgtccagcc gatgcacggg
    34621 ccttgaatcg gttgttggcc gcaggggaac tcgatgtcgt gatcggaacc cagatgatat
    34681 tccgacttgg tctcgagggg cgggcgttgt gtgtcgcgct tcccgacgcg gatgccggtc
    34741 tccacattcc cgatttccgt tctgcggagc ggatgtatca tgcccttcga gatgccgccg
    34801 agctggcccg tcccgcctct gagggtggcc ggcttctcat acaaacgcgc ttggcagacc
    34861 atcatgcgat tatggcgctg tcctgcggtg acgaagaggt gtttgtgagg caggagcagg
    34921 cctttcggca gatgcttcag tatccgccct ggacccgcct ggtcaggtta gatgtgtccg
    34981 gaacacagga ggccatggta tctcaagccg ccgatcgctg ggcggcattg cttaaagccc
    35041 aggcggcagg cagggaaccg atggttgtgg tcttgggccc atctcccgcg ccgcacgttc
    35101 tgaggcgcgg tcggtactgc tggcagattc tgatcaaatc cgcgtctctg gaggcaggga
    35161 aggagatagc cgtgcgaacg cgtgaggacg tggagcggga atcacgccgt ggtggcctcc
    35221 gttatgatat tgacgtcgat ccggtctcga tggcctgagg cgagtcaatt ccgggagcgt
    35281 ttcttcttgc ctcttggtgt ggcctgttgt ccctgctgca ccggaaccgg tggcgcgtgc
    35341 tgtgcccgca gccgtttgaa cagaccaagg gcaaacgtca cccaaaacac cgaggtgaaa
    35401 aggccgaaca gcacagcatt cagaatcgtt cccatctgtt cttccgtcgg ctgaatccct
    35461 tccacccgga tgcgtaccat ggtcacaatc ggccaggtca tgctttcgat accaagcccc
    35521 aatgttgtcc aggcccatac ggtcccgatg ctccgccctc gccagaggag aaatgcggtt
    35581 gcggcaacgg cgacaagcag cgtccagacg agtcctgcct ggtcccacat gacaaagatg
    35641 ccgccggcaa gcaccaggcc gcccagtagt gcgttcagtt ggggagtcca ccaggtcatg
    35701 atcacggtct cgttgaggag tacggggaga cgaaatcggg cgtagtgtgc ggatggatcg
    35761 gccggaagtc aatggactgc cgtgccgcga acatcgagtc ggcgtgtttc gttagccggc
    35821 aatctctgcc tgaacggcgt cgagcagctg ttgcatctcg aatggttttt ggagcgtgcg
    35881 gcgggcgccg agcatgcggg cgacatccag aaaattgagg atgccgatca tgtcgcttcc
    35941 gccggtgatg gccatgattt tggcgttggg gaattctcgc cgcaaggtca aaatgctctc
    36001 caagccgtcc tggtccggca tgagaatatc catgatcacc aaatcagcgg gcgcttgacg
    36061 atagtgcgac aaaccttctt tgccgtcggc ggcttcttgg acacgatagc ccgcctgctc
    36121 gagcgtgcta cggataaggc ggcgaatcgc ttcttcgtcg tccacgatca aaaccgatgg
    36181 cataacgttc ctctcaggag ttctcgtgat ggctctctgg gggcaagtgg tgcggccctg
    36241 cagaggtggt aattcggatc tctatgagtt gccggcactc atcgcgaatc tatcgctctt
    36301 tgtcgcgctc agcaagaaat tccttctgtc ctgtgatgtc tgatgcatct gcgcgaggga
    36361 gctctggtgc gccgatggcc ctgggtggaa gtatcgcagg caagtagact tcgatggtcg
    36421 ttccctggcc gactgtgctg gtcgagtgaa gtgttccccc atgctcactc acgacgcgtt
    36481 gaacccctgc gagttccgtc ccggcctggg ttccatccgg aaggtgggcg aacaacggtc
    36541 ccactttatg acgatggtct tgggcactca cttcctcgcc tgtatcagac actgtgaggc
    36601 aaacgtagtg gccagggcga aggggaaggt cctctgcgtt cgtcgattgg gtcaggtcga
    36661 cattgtcgag gcgcacttca aggatgccgc cgtccggttt catggctaac tcggaatggg
    36721 cgaggagctt cacgcaaact tggtgaatct gtgtcgggtc ggcataaatc ggatgggtgg
    36781 ccccgggaat ccactcgcgc agggtaatgt tatccggcag cgtccctttc aaaagcctca
    36841 gcgtttcttt caggaggata tcgagcgaga ccggctgctt catgccctcg gcctggcggc
    36901 cgaacatgag catctgggtc accagatctc tcgctcgctt cgatgcaagg accacttgct
    36961 ggacatgccc gtagacgcga ctgtcgggca ccagtaacgg cagggcgagg tcggagaatc
    37021 ccatgatggc cgttaggcag ttgttgaact catgtgcgat gccgccggca agagtcgcga
    37081 ccgcggccat tttgtgcgcc cggcgcacct gcgctgcggc atctttttgt gcggtgatat
    37141 ctgtggccaa gacttgaatg gccgggtggc catcaacggt aacctggctt gccaccactt
    37201 ccagatcaag gcgcgttcca tcggggcgta cgaattgtcg ctcgacaggg gtggcgcctt
    37261 ccgtcccatg aatggggttt gccacgggaa gatcctgcag gaatgccttc gggaaaacgt
    37321 ccgacaggga atgtccttca acagaggccg ctggcggtac actgaagagc cgcatgcctg
    37381 cgtcattcat gaacagaatc gtcccgtctg catagaccag gaccggatga ggcgacagcg
    37441 cggccaagct ccggtagcgg tcctcggcgg catgtaacgc ttcgagtgcc aggtgtcgcg
    37501 gggtgatatc gcggatcatg cccacgtatc ccgtgggggg ccccccctcc gtgcggagtg
    37561 gaaacgcctc ggccatcacc caccggagtg atccgtctgg tctgcaaaag cgaaattcgc
    37621 gtgaaaagct ccgggcctgc tcggtggccg tcaaccattc ggtgacaatc acgtcgtggt
    37681 cttcaggagc aagagccgtg agccagcatt cgggtgatgc cggtgcggta ggaagtccgg
    37741 ccatacggcg cagccgttca ttgagataga gagtatgccc ttccgggtcc gaaaggaaaa
    37801 tgccgatcga agcctggctg gataaggtac ggagaacggc ctcaatcgac cggaaatctc
    37861 ccgaggaagg aaaggcccct gccaagtctt gcgcgctcac acgtcactcc ttcgtacgca
    37921 ctgagggaac tgatgcggcc agcagtgccg cccaccgtaa agtttgtccg gctggtggta
    37981 atggccctcc agaatcatgg ggagcacgaa tacgtatctc agtgcgcttc atccctgcag
    38041 gattcgcatc atatcaccaa gccggatcgt gcggtgagtg atgattgaaa ttttgcaacg
    38101 taattgggcc ctagtgcagt agggccgttt atctgcgtga agggcggcat cgggtgccac
    38161 cagcccatag gtattcgcag tgagaatcag atgtgaatcg atgatgtgtg tgctggaaga
    38221 aggagactcg ccgagggttg cttgcctttc cgtcccccct gcaggttgca gagactggaa
    38281 ggcccttctg ccattgctga gtttggtgcc gaaggcggga cttgaacccg cacggcttgc
    38341 gccacacgcc cctcaaacgt gcgtgtctgc cattccacca cttcggcaac cgaatcgctt
    38401 tccgtcaaca catgaggcag ggtatgacga gccgaacgcc gatactgaat gggccgttct
    38461 tgctacgtag tccccggacc tcagaggaag cgcattatag aagtggtcca aaatgcttgt
    38521 caatcaacta gttgtccggt agaatctgac gcgtgctgcg tggacacgtg gcatcccctt
    38581 cccatataca gcgatcagcg gaccaacgtt cgagatgacg cctctctcag ccccacatat
    38641 ttcatgcaca ggtcggcaga cggacatcgc cgcgctcttt gacgtcgaca atacgttgct
    38701 ccccggacaa gccagcgaag tgcgattttt tcgctttctc tggaagcgag gactcgtggg
    38761 ctggcgtgag gttcgggaca gcattcactg ggtgctgcgg tgtgcgcctc cggtttcgtt
    38821 gcatccgttg cgggagcgca agctctatct ggccggaaag ggtgcggctg aggtcgaggc
    38881 gttggccgaa gaattttgcc gtaccgaatt atgtccccgt ctgtccgccc aggggctctc
    38941 tcgcatggat gagcaccgtc gagccgggca tcatattgtg ctggtgaccg gatcgctcga
    39001 tttcctgatt gctccgctgg ccgcattgct ggaggtgccg accttgctgg ccgccaggct
    39061 ggaacagcag gaacagcagt ttacggggca tgtctgcgcg cccctcccgt atggccccgg
    39121 caagcgggaa ctgattgcgc gtctgacgcg ggaatcaggc attgatctgg cgcagtcctt
    39181 tgcctacggc gatagtcccg gcgacgtcga gttactcgag atggtcggtc atccgttggt
    39241 cgtgaatccc attcgcggga tggaccgtat cgcgcaacga aacgggtggc cgatgaccac
    39301 atggaaataa agattgccgc aggcccatcg tcacgaagtg atcagccaga tcgatccacg
    39361 atgctcagag taacggaaat atttcacagc attcagggcg agtccacgca cgccggtcgg
    39421 cgctgtgtgt tcattcgttt gacgggatgc cccttgcggt gtacctggtg tgataccgcc
    39481 tatgcattct acggcggtca ggatctgacg gaagaggacg ttgttgcgca ggtccgggcc
    39541 ttcgggtgca atttggtgga agtgaccggc ggtgagccgc tcagccaggc ggcatgcctg
    39601 ccattgttga ggaggctctg tgacgagggg ttcgaggtgc ttctggaaac gagcggggct
    39661 gtcgatacgg ccggcgtgga tgagcgagtc cacgtggtgc tcgatgtgaa gtgtcccgga
    39721 agtggcatgg tcgatcgcat gtactggccg aatgtggatc gactcacacg tcgggatgaa
    39781 gtgaagtttg tcatcaaaga ccgcacggac tatgagtggg cgcgcaaggt ggtgcgcagc
    39841 cgggatcttg aggcgcgttg cacggtgctg atgagtcccg tttttggtga agtcgatgcg
    39901 cgacagttgg cggaatgggt gctggctgat cgattgccgg tgaggtttca gttgcagatg
    39961 cataagcaga tatgggcccc cgatatgcgt ggcgtgtaag ccctgcggat tggccccgtc
    40021 ggcagaggaa agaatagaga acggcaagaa ccagggagga ttgatgaaga agatccaagt
    40081 tcaagcacag gttgggcggg cagaacaaga aggtgccgag gtgctggtat tgctctgttg
    40141 cgagggggcg tcagaccttt cgccggaagc tgccgccatc aacacccagc ttggtggaca
    40201 gctcgcgacc ttgattcagc gcggtgaatt cgaaggcaag cttggcgagg ggttgctcgt
    40261 gcacacgcag ggcaaggcca aggcgaagcg gttgctcctg gccggcttgg ggaaagaaaa
    40321 ggatttgcgc ctggacgcct tccgccaggc attaggcgct gcagtcaaac gcgtccgcca
    40381 ggccaaagtg tcttcattcg gcgtggttct gccgggtgcg gttctggaag aaattcctgt
    40441 tcaggatgtg gcccaggcca tggccgaagg ggccatcctc ggcaactacc aatttacggc
    40501 gtatcggagt tcgaacggaa gcaaaccggt agaggtcgag cggctgacga tctacgtcgc
    40561 acaaacatcg ctgctggcgc aggtcaccga gggcatccgg cgcggcgtcg cgacggcgga
    40621 ggccacggtc ctggtccgcg atctctgtaa ccatccggcc aatatcatga cgcccggccg
    40681 gatcgtgcag gaagcaaagg ctgtggcgaa agaaccgggc gtcaagctga aggtgcttga
    40741 acagaaagat atggaacagc ttggtatggg ggccttgctc ggagtggcga agggcagtca
    40801 tgagccgccg aagttcatca ttctggagta caagggcgcg aaggcgaaga agggagatcc
    40861 cccggtggtc atggtcggca agaccattac cttcgacacc ggcggtattt cactcaagcc
    40921 ggccgagaat atggaacaca tgaaggccga catgacgggc ggcgccgagg tgctggcgac
    40981 gatgcgagcc gcggcacgac tcaagttgcc ggtgcatctg gtgagcatat tgcctgtggc
    41041 ggaaaatatg ccggggggtc gcgcgatgcg gcctggggat atcgtgaaga cgctctcggg
    41101 gaaaaccgtc gaagttcaaa acactgacgc cgagggccga ctgatcctct ccgacgcctt
    41161 ggcctatgcc acccgctaca agccggctgt gttgatcgac atcgcgacgt tgaccggtgc
    41221 ctgtgtcgtg gcgctcggtc aatttgccat cgggatgttc ggtaacaatg atcagttgaa
    41281 ggaacatgtc cggaatgccg gcatgcgagc tggtgagcgg gtgtgggaaa tgccgctctg
    41341 ggaggagtat ttcgaacagt tgcgcagtga tgtggccgat atgcggaaca tcggtggccg
    41401 tggtggaggc atgattaccg cggcactgtt cctgagcaag tttgtcgggg actgcccgtg
    41461 gatccatctg gacatcgcca gcaccgactg gagcgagcgt gagcgagcct atctcccgaa
    41521 aggtcccacc gggatcggca cgcgcctgct cattcaattc ttgctggacc ggaccctgcc
    41581 ctagcggcaa ccggtgcgtg aggcggcgtc agccgagcat ctctctgctt ctcacccaga
    41641 tcgtcatcgt agggggttat gacattacgc gagcacatcg gtcagctgtt catgatggga
    41701 tttaccggga ccacggtcag caaggacctg gcctcgtttc tcaccgccta taagccgggg
    41761 ggcgtcatct tctttcgtcg caatctcgaa tcggtccagc agatcgtcga tctcaccaac
    41821 ggactgcaga agttgtcgcc ggcctcgccg ctattgatcg cgattgatca ggaaggtgga
    41881 cgggtctccc gactgccggc cgaatttacg atctttcccc cctgcggtca gctcggccag
    41941 tgtcattcca gtgaactcgc ctattccgct gcagccacca tcgccaaaga attgcgagcc
    42001 gtggggatca acatgaatat ggcgccggtg ctggacgtga acagcaaccc cgacaatccc
    42061 gtgatcggtg atcgggcgtt cggtgccgag cctgacgtcg tcgccgacat ggggctggcc
    42121 acgatcggcg gcttgcaaga caatatggtc gtggcctgcg ggaaacattt tcccggccac
    42181 ggtgatacgg caacggattc gcacaaggag cttccggtcg ttgacgcggg gattcaacgg
    42241 ttacgcgaga cggagtttcc cccgtttcag caggcgatcc ggcagggagt tgccagtctc
    42301 atgacggccc atgtgttgta tcgggccttg gatcccgatg cgccggccac gctgtctccc
    42361 gctgtgatcc agcggctact gcgcgaagag tttcgttacg acggggtggt cttcactgac
    42421 gacctggaga tgcatgcgat tatcgatcat gacgggatcg gcgaagcggc tgttcgttcg
    42481 ttcgtggccg gatgtgatgt gttgctgatt tgcaaagacc aggaccgggt tatgacggcc
    42541 atgcaggcga tggaacgtgc tgtgcaaaac ggccggatca cacaagaaag actggagcag
    42601 tccttggccc gtgtcgcacg actcaaggca cgatacctga tcccctacaa gccggtgacg
    42661 atctcggatg cccgtctggt ggtcgggtgc cgttcgcaca aggtgttgct ggactcatgg
    42721 cataaagcct atgcccggct gccttcctcg aaggtgtcgg agcacgatga gccggcggcg
    42781 gcgttggatt caccggtcac gcatgtctaa cgcatagccg tatgcgggtg gggcatcgtc
    42841 tgaagcagcg gcgcgagggc caatctcctg agaggaaccg gtgctggatt ttcctctcga
    42901 gttttgattc actagtgctg gattgggctc cattccatcc attcatcata ctgaggcgcc
    42961 atgggcatca agaaaggcga cattctcgac gaacggaaac gctttcccga ttcctgcggg
    43021 ctggtggtta agtgtaccgg tggcggcgag ggttttcaga agatcttttg ttgtgggcat
    43081 gaactgactg aagaggacct cgtgtcggag atcgtatcgt cgaggggccg gaaacgagga
    43141 accttactgc cgggggccat gctggaagag cggcgacagt ttcctgattc ctgcggcctt
    43201 cgcctcatgg tgatggatgg cggcgccgga ctgcaaggca tcgactgttg cggccatctc
    43261 attacggccg gggcggtgca tggcttgaga ttcggtcaac cgctggatga aggggctcct
    43321 atgccgaccg gtccggcagg gcaggcgtaa tgcgcgcacc cagtatgccg ggggcggatg
    43381 agaagtttca tcgacccagt atgcgatggt ggctcgggtt catggatcag ctggaccggc
    43441 ttggctatgt tgcggccgga ttcagtctgc tggcgctctg cctgatcatt ttcgttcatg
    43501 cctggtatat cttcctggct cgacccgttc agctcgcgct ccttcctgcc gccctcaaat
    43561 tattgaacga tctgctcctg gtcatcattc ttttggagct gtttcgcacg gttgtgcgat
    43621 ttcttcagac ggaagtgctg gaattagagc cgtatttgtc cgtggggatc attgcctgta
    43681 cgcgaagact gttgacggcc agcgccgaac tctcctacca gcttgaagcg gtggctaagg
    43741 aaagcaggca cgaattattt cagcaatatc tcctggatgt cggcttgaat gtgctggtca
    43801 tcatcatcct catcggaggg ctgtacctgc ttcgctcccg tccgtcgacc gaacgtccgg
    43861 ccgtctcctg agcgggagtg gaccgaatag cagggggtgc taggtccttc ttgccctctt
    43921 cctatctctt atggcatcat gcacctcttc ttggaggcat ggtggccaca ttgcttgaat
    43981 atgtgggatc ggtccatatc tacctcgggc cctaccgagg aaatcctatt gccttgtatc
    44041 tgaggcgtac ggaggtggga tgtcagatcg ggcctcgcgc ctatccatgg aatgatgtga
    44101 taggagccgg ggaaactcct aacaaagccg ctgcggattt cgaggaaaaa tggaagggca
    44161 aggggctggc tgctgatatg tattcaggcc cgtcctggga aggtggaatt aaacccgaac
    44221 ggcctgcccc tccaaaaccc gcggctcccc cgaagcctgc ggccgctcct gcgtctgctc
    44281 cagtcacttc agctgccgca cctgccccag ctgcaccggc agcggctgct gccaaaccgg
    44341 ccgcccctgc tgcggcccct gtcgcagccg cacccaaggt cgccccagtc gcgccaaccg
    44401 aagcgtcgtc ttagtttctc catcgtcgaa gtgttcatgc agcggggtca tgcgcgccga
    44461 gcgtgactgg tgtgttcaag cgccggactt gtggcgagtt tgctgcgccg tatgccgtac
    44521 gtggcgtaga caacgattcc gatgaccgtc cacacaaaaa accggatcca ggtcatccag
    44581 ggaaggaaat acatcaagcc taagcaggcc agcacgccca gcatgggaat gaccggcatg
    44641 aatggcatgc gaaacggccg aggatggttc gggttggtgt aacgaagcac aatgattccg
    44701 atacagacga ggacgaaggc gaagagcgtt ccgatgtttg tcatatcggc cgcttcgcca
    44761 atgggaatca aggcggccat gatggccacg gctatgccgg taaggtaggt ggcatggtga
    44821 ggtgtgcgaa atttcggatg cacgcctgac agccagggac cgagaagccc atcgcgggac
    44881 atggcgaaaa acacgcgaat ctggcccagc atcatcacca ccagcacgct cgtgatcccg
    44941 gccactgctc cgaccgctac gatggcggcc ccccatttga agcccgcgac ccgcaatcct
    45001 tccgccaccg gggcatggat gtcgattcgc gaaaacggca ccaggccggt caggaccgcc
    45061 gccacggcaa tatacaggag ggtacagata cccagcgagg cgaagatccc aatgggaaga
    45121 tcgcgttgag gatttttggc ctcctccgcg gtggttgaga cggcatcaaa cccgatatag
    45181 gcaaagaata cgatggccgc cgccgcacct acgccggcaa atccgaacgg catgaacggt
    45241 gtccagttgg tcgtatcgac ggaggaggtc ccgacggcga tgaaaaagac gatcacggcc
    45301 agcttgatca ggacgatgcc gcaggtggct cgggcacttt cctttacgcc gacgatgaga
    45361 atgccggtga ccaataggac gatgatcgca gccggaatat tcgcgattcc tccgtccgct
    45421 cccgggggat gcgtggccca gtaaggcagt tcgaggccac acagttttag aatgttgttg
    45481 aagtagcccg accagccgat ggcgactgcc acgcaagcca cgccgtattc tagaatgaga
    45541 ttccatcccg tcagccaggc caggaattcc ccgagggtcg cataggaata ggtgtacgcg
    45601 ctcccagcca ccgggatcat ggccgcaaat tccgcataac agagcgcagc aagggcgcag
    45661 gtgatgccgg atagaatgaa cgacaggacg atccccggcc cggctcctgg tcgatgcgca
    45721 tcgccgacga tcgcggtacc gatcaggacg aagatgcctg tgccgatgat cgcgccaatc
    45781 ccaaggccgg tcaggtccca ggcggtgagg cttcgtttca gacgatgttc gggggcgtcg
    45841 gagtcagcga ggattcgttc gatcgatttg gtgcgaaaca gcggattgcc cacgccgtgt
    45901 cctctctccg gtgacgtcac tcccatgtgc gctggcatgg gcgaatgagt cccctctcat
    45961 gtggtggtcc cgcgctgcgg agacaatggt cgaagcgccg gacgacggga ggctgcccgt
    46021 aagaatgcct caaagagctg ccgatgcagc cggtggcgct caaacagaaa ttctggatgc
    46081 cattgcaggc ctagaaaaaa tgggtggccg ggatgttcaa tcgcttcgac gatgccgtcg
    46141 ggagccgtcg cgctggcgat caatgcgcgc cccacgactt tgaccgactg gtgatgggag
    46201 ctgttcacct tcatgcgcgg gcgtttcatg atgcgctcga ggagcgtgcc gggtgtgatg
    46261 gagacgctgt gagcgagctg aatagccggt gtcgtctgcc ggtgttgcag tacatcgggg
    46321 acctgggctg gaaggtcctg atagagagtc cccccgaatg cgacattcat ggtctgcatc
    46381 ccgccgcaga tggccagggt cggcagatga ttgcgggttg cgagatgaac gagatccaat
    46441 tcgaagctcg agcgtcgctg ggcgacgatc ggaaacgaat attgcttccg ttctccgtag
    46501 agcgcggggt ctaagtctgg accgctgccg gtgagcagca gcccgtcgat ggtgcggagg
    46561 aggcggcgac gcgccgcacg atcagccaca aggggaagga tgagcggcac cccgccgagt
    46621 tcttcaattg cgcggacgta ccgcgcccgg agaaaatagg tgggctcttt gccgccccat
    46681 tcctgacgat caccggcatt gaagtcgggc gtgactccga tgacgggttt catactatcg
    46741 gatcggttcc aaagggagcg gcaggggctc ttgaggggta tagtccccgg cttcccgcgt
    46801 gggttcgctc gaggaaacac cgaggaagcg aatcggctta tcccccttca gatggtccgg
    46861 cgtaatgatg taggtgccgt acatgctggg gatccaaata ttctttgcgg tttgttcgct
    46921 cccgcctgag aagccgtacg ccagtccgcc aaccactccg ccaccgatag caaacgcgag
    46981 tttgagcggg aagtagatga tggttgccag cgcggatcct gcttgaatgc cgacccctga
    47041 agggctggca tcgttcgagg agacgggaac gttggatgcg ctgcccgatt cttgtgctgc
    47101 caccggcaca atgaggatgg ccagtgagca aatcgccacc agaccgagaa ccaacgcgtt
    47161 tgaccagagg ctgcggcgtc ttttcgagga cagattgaca gagacgttca cgttaagtgc
    47221 ctccttcgtg ctgaaatgct gacggtgtga aattagaaga tgagtgccga tgaacttcta
    47281 caatagcgag tactccgttg tcgttataac aaattcatcc ccattttcaa accccttccg
    47341 cgtttgtcgc aggtggtcct ccgctcttca acgatcaggc gactcttcca ggtctaattc
    47401 caggcgaatg cgctccatca cctgttcggg ctgctcggtc atgatctgcc ggagttcatc
    47461 ggtaaagcgt gccgcgtcga tcacctggtt atgttgctca atgcctccgt ggagagcgag
    47521 attgagtgcg caggtgcata cggattggat aaagagttca tccgcccgac gcgtcagctc
    47581 agcctgttcc tcttggcaga tcttgtgtaa ttgatgttca acccaggttg aaaagccgat
    47641 cgcccggcgc gcctcctcga cgtgcttcat agcgatgtcg aggctgacct gtatgccgcc
    47701 tttccaactg cgttttttca cattaaacac gcagcgcagg agatcgggcc gatgctccac
    47761 cggttcgggt ccgaaaaaaa atgtgacgcg gtactggctt ggttctgtcg cagcgggggg
    47821 aatactcata ggtcgtggaa agcattctat cattccggag cgcggagagc atgtctattg
    47881 tcgagtggtt tcagtttcga aatgacgtta tgatgcgatc atgaaaacgt ctgctgattt
    47941 cgtggctcag gtcgatcgta ttcaagaagc cattcgggca catcccggga ttgacgggtg
    48001 gctgttttat gacttccgcc atctcgatcc cattgcctat cgcgtgttgt tgttagatcc
    48061 gtcgatgcat gttacgcgcc gttggtacta ttgggtgccg gcggtgggga tgccggtgaa
    48121 attacagcac cgcattgaac cgcatgtgtt ggaggcgttg cctggtgagg tgcggttcta
    48181 tgtctcctgg cgagaacagc agacggcgct aagggcgctt cttcaatcgg cgaaacgcat
    48241 tgccatgcaa tactcgccga tgaatgcgat tccgtatctt tcgcgagtgg acgctggaac
    48301 gttggatctc ctgcgcagct ggggtctgga gattgttacc tctgccgatt tggtccagca
    48361 atttgaagcc gtgtgggatg aggcgcagtt ggcgtcgcac caggcggctg ccacggggct
    48421 gcgagcgatt gtggatgagg cgttcgggtt tatcgggaca tccctcgcgg caggctcttc
    48481 gctcaatgaa tacgggttgc agcaatatat cctgtctcgg atgcatgctc gcggcctcgt
    48541 cacgtcgagt ccccccattg ctgcagtcaa cgcccatagt gccgatcctc attatgggcc
    48601 atctgccgag ggctctgctc ccgtccgcca gggggacctc gtgttgatcg atttatgggc
    48661 taaacagccg gaggccaggg ccgtgtatgc cgatatcacc tggacaggtt tcgtgggcaa
    48721 ggtcgtgcct gccaggcagc aagatgtctt tcagatcgtt cgccaagcgc gagatgcagc
    48781 ggtgacattc gtgcaagcgc gcgttcgaga gggcacattt ccctacggct gggaggtcga
    48841 cgatgtgtgt cgacaggtca ttcaacaggc tggatacgga gagcattttg ttcaccggac
    48901 tgggcattcc ataggtgaag aagtccatgg caatggcgca aacatcgaca atctggaaac
    48961 gcaggatgct cgccggttgc tgcccggtac ctgtttctcg atcgaaccgg gaatctatct
    49021 tccgggagac ttcggtattc ggagtgaatt ggacgtgtac ctttcgacca atgatgcggt
    49081 ggtattcggt caaccggtac agaccgaact tgtcgcgatc acccttccca ccacgtgagt
    49141 ctggtctcat cctgagggat ggcctccgcc tttcttgaca ccctttgcat cgggcagcta
    49201 tcgtgagaga tcgactggcc gcagaggtct cgtgcctggt tggcccctat ttgccgagga
    49261 aggataccga acatgtttgg cacgatggga ttctccgaac tgattatcat cctggttatc
    49321 gtcttgatta tttttggtgc cggcaaactt cctcaaatcg gggagggcgt gggcaaggcc
    49381 ctgcgagggt ttaagaagga agtgaatgat atccctgctg ccgatgccac tcctgaacag
    49441 ggtgaagctg caactccttc gtctcaggca cagataccgc ctgttgcaga agttgcccag
    49501 actgctcagc ccgcgccggt ggctcaggcc gctcccagtc tcaaacctac cgcgccctac
    49561 acgcctggtc cggagttaac acctgggacg acagcagcct tgatggcggc tgctgcgcct
    49621 caggggcctc aaacggccca agcgtctaag ccccgggtcg ccacggtcac gccaacacag
    49681 gctgcccccg gctcggtggt ggcacagggg catcaacctc cgacgatgga agatcgcatg
    49741 gcggctccgg caccggtgat gcggggacag tatccccctc ttcctgcctc ggctcagagt
    49801 aagccggtgg ccaagcgtcc ttctgctatt gttaataaag acgcagtggc tcgggtacag
    49861 gctgcgcagg ctgctatccg ggcaaaagca gcgcagcctc agccggccgc cttttctccg
    49921 caagacatgc aggggcttgg cgagggactg ggagacgccg tgcggacttt ccggcaggcg
    49981 gtcgcggatg tgcggagttc ggttgatcct cagatgcgga ccattcgagc agaaatggat
    50041 tccgctcaaa aggaactgga acagtcgatc gaggcagcga agcaagcgcc cgtgctcgac
    50101 gaggacactc ctgccaagcc gctctaaccg cgtggagttt ggatagcttt cgtttggatc
    50161 ggtaatggga tgtgttgccc caggggactt acagcttgat gcgtgcaggg tctatatcgt
    50221 cgtgctgggc cgatcgacat accactgtga aggaaattgt cacctgattc ccggtctatt
    50281 cctctcgtgc agcatgcccc gaccatttta acgctcacat gagttccgaa tcattcctcc
    50341 gtaacccgtc ctacctcgca ttgttattgt tgttggtagt ttccttgacc atatccggtt
    50401 gctatgtgga agtcccgcca gcgactcccg cggtggtttc cgtgaggctg aacgaattac
    50461 tcgctgatcc cgatccggaa gtgcgtcgca cggcggcaga agggttggga aagatcggcc
    50521 acccatcggc aagcccaggg cttattgctg cgttggcaga tcgagatccc cacgttcgcg
    50581 cagctgcagc actcgccttg ggtcgagtgg gtgatgcaaa gagtggggcg gcgcttgtgg
    50641 aacatttgtc ggattcgtct gagtctgttc ggactgcctc cgccttggcg cttggcgaaa
    50701 ttggaacttc cgtcaatcat gaggagaaga ttcttcgcgc actccgccag ccggaggttt
    50761 ctacccggct ggctgccagt cgagccctcc tcagtttgga gacggtttca ttttcaacgg
    50821 atctcctgtc ggccctggac gatcccaatg cgcaggtgcg gcaaggagtt gtagcggctc
    50881 tcagtgagac aggggacgtg agatctttac caggtctagt gaagcttctg caaaacgatg
    50941 cggatgcggg agtgcgtgct gaagttgcgt ttcgactggg aaaagtggga gatgccggtg
    51001 tggtgcctgc gttatccgtg gtagccggga aggattcgaa tccgacggtt cgacaatggg
    51061 cgcattgggc tgttcggcag attacgacat cgcccggctt cggttcaggg aatcgacaag
    51121 gtcaatgagg cgagtctgag ccaccggatc aatgtcggta aactgaatgc ccatacctgg
    51181 aaagagcaga taccgctctg gcttcgaccg tgtccaggct acttttgcac gtgtggcaaa
    51241 tttctggttc ggtcggtcag gtagggcaaa ttccacggtc agttctgtgc ccggtgccaa
    51301 cggcgtacta ctttcgataa acaatccccc tccgccgatc ccgccagtca agctatcaaa
    51361 atgcttcccg tcctccgtgg tgcaatggac tttgatggca aggggagcgc gtggatgcga
    51421 tcgactatgg gcaaagcggg catcttcatc aacggagagc atatgctcga tgagggttcc
    51481 ccagcttagg atactgagct gttgtccctt gtgatcgaac aacgatacgg tttcctgttc
    51541 gcaatcaata ccgagcgttt tgccgtggtg ctctcgagtg gcagtgacgg gaaatttcat
    51601 cggtgaggtc tcggagggtc cgcagtgaat cgatctgtca cgaataggaa tcgtgtgtcg
    51661 agagtgttta gcaggactac tcgctctgcg gaatgccttt tacagaatga accagattca
    51721 gcacgcgact tcgggtctct gatgcgatat ctgtgaaacg tacacccata ccagggctaa
    51781 aagtatactg gtcggccttg gggcaaaccc aggcgatcac tcccttggcg gacatccact
    51841 cgctggggtc ttctggtagc gtgaattcca tggcaagttt cgttccgact gccaaggggg
    51901 cgaggctctc gataaagagt ccgccaccgc cgatgcctcc ggctcgactc tcgaactgca
    51961 tgccatcggg ggtgtggtat ttgacgcgga tagaaaggga gatccgcggt tcagaacggg
    52021 cttcacggga ttgctggggc tttcgatagg tcaggatctg atcgacgaga aaatcccacg
    52081 cgaggcttcc cattggctcg ccgttgatgc caagcaacgt gatcgattcc tttgaggaat
    52141 caatttcaat cgtcttgccc ttgtgtcttg tgctggatac gacggggaat ttcacaagcc
    52201 gccctttaca aacctacatg ttgcaacaag gcatctggtg caagtatagc ccatggatcg
    52261 aacttgtcga taaaaacctg cgattggaga agtttggcga aggagaggtc ggtaatctac
    52321 tgcgaagggg aataaaaaaa aagagggggc ggacgccccc tcccgatcgc tgaaatgcgc
    52381 ttaatagacg agtagttttg aggggttagg cgctcttgac gtctttgtcc tgttcgaaaa
    52441 tccgaatagg cgcgtgtttt ccgctgatgg catcttcagt gatcacgact tccttgatct
    52501 gtttctgcga cggggcgtcg tacatcacat ccagcatggc ctcctcgaga atcgcacgta
    52561 acccacgggc accggtcttt tgagtaaagg ctttccgggc tacagcactt aacgcacctt
    52621 cagtgaatcg gagcttcact ttttcgaagg acagcagctt ctcatattgc ttggtcagcg
    52681 catttctggg ctcggtgaga atccgaatga gcgcttgttc gtcgagttca tcgagcgtag
    52741 caacgaccgg caaccgacca ataaattcgg ggatcaatcc gtacttcaac agatcttccg
    52801 gctggacatg cgggagcagt tcgcccaatc gaatatcatt gcggccccgg acctcggctc
    52861 cgaaccccat cgatttccgg ttcagacgct gctcaataat gtgttcaagc ccgacaaatg
    52921 caccgccgca gatgaacaaa atattggaag tattgacctg aatgaattct tgatgcggat
    52981 gtttccgccc gccttgaggg gggacgttcg cgaccgttcc ttcgatgagc ttcagcaagg
    53041 cctgttgtac accctcgccc gaaacgtctc gcgtaatcga cggactgtca ctttttctgc
    53101 tgatcttgtc gatttcgtcg atatacacga ttccgcgctc tgcccgttcg acatcgtaat
    53161 cggcagcctg gagtaatttg agaatgatgt tttctacatc ttctccgaca tagccggctt
    53221 ccgtaagtgt cgttgcgtcg gccagtgtga agggaacatc aagaaatttg gccagcgtct
    53281 gagccagaag ggtttttccc gtgccggtcg gtccgacgac cagaatgttg cccttttgca
    53341 gttcaacgtc gtcgacgtct ttgtcctttg cggagatgcg cttgtagtga ttgtgcacgg
    53401 cgactgagag gatacgtttc gcacgatcct ggccgacgac atactgatcg agatgatgtt
    53461 tgatctcggc cggcttcttc agcttggagg agatttcctc cttcgcttct tcccagtcct
    53521 ccgcaatgat gtcattacag aggtttacgc attcgtcgca gatgtagacg gtaggaccgg
    53581 cgatgagttt gcgcacttcg tcccggcttt taccgcagaa cgagcaacgc aggtgccgat
    53641 caatcttttc ctgtttagcc atgctgcctc ctgtacacca aaggtcaact agctcttgcc
    53701 gccatccttc cctgagtccg ttgagcccac gggtttaagg ctcttcaggg ggcgtgtaat
    53761 cacttcgtca atcaggccgt agcgtttggc ttcctcactg gacataaagt agtctcgctc
    53821 ggtgtcctgg gaaatcttat ccagcggttg tccggtatgt ttcgccatga tttcattcag
    53881 gcgttcacga atcttgagga tctcccgggc atggatgtcg atctccgttg cctgtccctg
    53941 aaatccgccc atgggttgat ggatcatgac gcgggcgttg ggaagtgcat agcgcttgcc
    54001 tttagtcccg gctgttaaaa gaaacgcccc catgctggca gcctgcccca agcagatcgt
    54061 gttgatggat ggtttgacgt attgcatagt gtcgtaaatg ccaagtcctg ccgttacgct
    54121 tccgcctggc gaattgatgt acagattaat atccttctcc gggtcctctg cctccaggaa
    54181 caggagctgg gcaataatca ggttggcgaa cacgtcatcg ataggagccc cgaggaagat
    54241 gatacggtcc ttgagaaggc gtgaataaat gtcgtaggca cgctcgccgc ggtttgtttg
    54301 ctcaacaaca atcggaacta acattcggcc gaatcctttc cgctagagga ctaacaggtg
    54361 gaaactcctc agtttaccat actcatcctg ccgggaattg gtgatgaccg cccgacctca
    54421 gtacccgcga ctcctgattt aagagcgttt agccctgaat catcgcgtgc ttgtatacaa
    54481 aatccaatgc tttgtcagcc aagattcttg accgaaggtc attcagggtg tcttgaccgc
    54541 cggcttgaat catgcgggtg acttcctcga cggagagttt cacttcagca gcgagccgcg
    54601 cgatttcgtt gctgagatct tcattggtca ccgtgatgtt ttctttcgtt gccagcgctt
    54661 ccagaatgag cccgaccttc acgcgacgct ccgcttccgg acgatattct tcctgcagct
    54721 gtttggcttc gtcggcttga gaggcaccgg gcgatccgtc tgccgcgtca ccggtcttcc
    54781 gttgacgaga ctgcaattgt tgacgaacca tcgcagagag ttctcgttct actagcgttc
    54841 cgggaagatc aaaatggtgg gtttcggcca atcgcttcaa aatcgtatct ttgtaggagt
    54901 cctcaatgtc ctttttcaag gcacggtcca tttcggcgtg gagcttctct cggatttcct
    54961 gcagagattg atacgggccg caatctttcg cgaattcgtc gtccagggcc ggaagttgtt
    55021 tgcgttttat ggacttgacg gtgcagcgga agttgacggt ctttcccgcc actcgcgcat
    55081 cgggatgtgt cgccgggtaa gcctggggaa tggtgatgac gtcaccctcc tttttcccta
    55141 ggaggtgcgg ttcaatttcg attccgagga cggaagactg agaacccatt ttatggagat
    55201 gtcccgtctt ggttgttcca tcgagcgcag caccgtccac tgtgccgtca atgtcgacaa
    55261 ccgcatagtc tccatcggcc agagtcgttc cggctggcgc gggatgcaac tgagccatct
    55321 gctcacgaag aacctcaaat gccttttgca cctgttcatc ggtgactgtt ctctgatcct
    55381 gttttagaga gattggattc ggagctttgt aatcccgaag ctcaatcgtt ggcttaatct
    55441 caacggtagc cgtaaagcta aagggggagt ctttcttcac tttgacccgt tccaacggcg
    55501 gtatttctac aagaaccgga acgatgcccg cctgccggat agctcgatca taataatccg
    55561 gcaccaagct acgaatgaca tcttcttcaa tcgttttggc gtagcgcttc tcaagcaatt
    55621 ggaggggggc ttttcccgga cggaagccgg gaatgcgaac ttgccgattc aattcagagt
    55681 acgcctggac aaaccgcagg ttcacttcgt ctgcaggaac ttcgattttg agcgctcgct
    55741 tcatggggcc gagttcggtc atttccatct tcataaggct attcactttc tccgagtccg
    55801 ataatgtttg gtgcggcatg ccaggtcata gaggtggtgc gagagggggg acttgaaccc
    55861 ccacggttac ccacgagatc ctaagtctcg cgcgtctgcc agttccgcca ctctcgcgcg
    55921 acgctgagta ctaggctgct cactgatggt gcgtaagaaa tggatataca tgtgtaaagg
    55981 cacgatgaat aatgacattt tgtaccgtcg atcgtgtctg cctgtcaacc catacgcagg
    56041 tggggcccgt gtgctgattt gtgattctga ggggaactcg gccgtagaga aaattgtatg
    56101 gagtgaaggc ctccatcagg cggttgtctc tctcgtgctg cgaggctcct tctcctggta
    56161 ttgccctaca tgaggaacgg tataatggac acacaggtct gattcactat cgccatccga
    56221 acgttcttcg aaataaaggg ggacaccgta tgaaacgcca gtctgtgaca gctgcacttg
    56281 ggtgtctcat tctgctgtgc ggcttcccgc tttcgagcga ggcctatcga gattatttta
    56341 ctgacgcaca aaagcaagaa ttagtaaaga ttcaaaccgt cttgattgag gcattggccc
    56401 taacagacaa aggcgctggc aatgccgacg gaatcttgga cgtggccatg aagcgcatgg
    56461 gcgagctcgg ttatacaaca gtctcggatc cacacgtgtc ccacgatgtc gtcgtgcggg
    56521 tcaaatgcga gcaaaagaag acctgggagg gcacggcatc cgcgggggga gacaatgatt
    56581 tgcctgatgc gccgtcccgc ttgtggaaag gtccggcatg tcagattacg tacgcgttgg
    56641 gtggtatgaa ggtcaaatgg cagaaggagg ttcggacaga atttgaagat gcggtcggag
    56701 ctgcccaatc ggctcaggca ccagacccgg ggctgtatgc catgaatgcg cttcatgacg
    56761 cgctggagac atatgagttc ccccttctcc tgaccgcaga gtggggccag cctgatcggc
    56821 tgcttaaatt actcgatagt ccgcaaacca gccaaattcg gaagctcaag attatttcct
    56881 tgttgggaga gatggttgct gatgaggcgt taccccacct caccgaggct ttaaaagaca
    56941 aagatctcgc caagcaagcc gcggtcgcat tggggaacat ggggaaggaa gggattcctg
    57001 tgctgatcga cattttgaag cattccaagc aacccgagtt gcaagccgct gcggctaaag
    57061 gtcttggtga tctaggcaac attcatagtg attccagagt agtgccgcct ttactggaga
    57121 tgcttgatgc gcccggcatc gacattgtag tgcaaacgga ggttgcgtgg gctctcgggc
    57181 gggtgccgga tcgacggtcg gttgaaccgt tatttgccct cgaccggaag ctgcaaaaaa
    57241 tccgaaaaga tcctcccgat ccccagatta agaaattaaa ggaggcggtc ttctggtcaa
    57301 tcaagcaggt gtataccgaa gaccagtaca gttagcagct aggcatgtat cctggtgcag
    57361 tattgccgcg ttcaatagtc cgtgacctct ccgcgatggc gtcgctatcc tgcgtcacca
    57421 gccttctcgt aatgggcgtg cagttcgcgt ccaatgtctg ccggtcctgc aaaatagcat
    57481 tcccaacttc gagctgtgaa aacagcggcg actctgctat gaaggtcgag cagtgtcgag
    57541 gaggtcgtgt aagtaggtca cattaactgg gccgggaaca tgccaagaca acaatgaggg
    57601 ataggacgat gagagatatc tcgcgacgac gctttctgca aatatccgct atgagtggtg
    57661 gcgtgctgct tttgggcgat ctcgccgagc gcatactcaa tgtgcctctt ctacgatcag
    57721 cggcgttggc ggcagagccg attaaaatcg gtattctgga cccgctatcg agcccctata
    57781 agacgtcttc gatccacgat gtacatgggg cgaacgtcgc ggtggatctc ttcaacaagc
    57841 gcggtggggt attggggagg ccggtgacga tcatcgaggc ggatgatgcc tccagccctg
    57901 atgccgcgct caaggcggcg acgaaattcg tgcaggacga tcgtgtcgat gtcttgatgg
    57961 ggacattcaa cgctgagtgc gcccttgttg tgtctgagta cgcgaagaag gcgaacaagt
    58021 tgttcatggt cacgggcgcg catttgcctg agttgacagg ggccgcgtgt aattcccaca
    58081 ccttcgtgtt tatgcccaat gcgtccatgc tggcgcaggc cgttgtcccg cagttggtga
    58141 aagcctatgg cactcgttgg tacatgatta cgacgagctc tttggatggc aaagcgatgg
    58201 ctcaggccat ggcctcgact ggtcttactc atggagtgga attcgtgggt gagacactga
    58261 tgcccttcgg ttcgacggat ttcacctcgg cactcagcgc ggccaaggac aaacgcccga
    58321 cgctcgtagt gttgaacctc tatggatggg atcttgtcca tgcccttaaa gcgtacacca
    58381 agttggaatt agccaaggat aaaatcggtg tcggcgggat gattgccggc gagcagattg
    58441 gccgtcccct cggctatgca aataacgccg ggatctgggg cctgatctgg gaccccaaag
    58501 tgaatactga cggatccaaa cgatttatcc agggagtcgt ggagaagtac aatcatactc
    58561 cgacatcgcg ttgttatctg gggtatgcgg ccatgacgca gattctggaa gcgattcagc
    58621 gggcaggcac aactgaagcg tccgcgctca tcaaggtatt ggaaggacat gagtttgatg
    58681 gcctgaaaga agggcgatcg tacttccggg cctcggacca ccagcatgtg caggatgtgt
    58741 tagtcggaga agcgtatggc aaggaactgg gactgggaca ctacaagatt ctggccactg
    58801 ttgccgggga gtctgtcgcc acggtgcaat cgggtagttg ccagttgtaa gccgagtcgt
    58861 aatggtcggc tctagtatga gggagcaagt gcggcgagac gatctcgctc ctcagttgtt
    58921 ttccccgtcg tcgatgcaac ggtaggtcct tgcacaatga ccaattcgag gcggcggttt
    58981 ttctggcgac cctgttcggt tgcgttggaa gcgataggtc tggagtcggc caaaccgacc
    59041 gctttggttc gctccggaga catcccgccg gtgaccaatg ccctgcgcgc attttccgcc
    59101 cgagcccacg acagcgcctt attatccgaa aaggttttct gaagggtttt gctcagcgcc
    59161 tgattgtccg tgtgaccggc cacttgtaca aacttgtctg aaagcggtcc cagcacagtg
    59221 ccgatgcgtt tgagcatcgt catgccttca gaggtcaagg cggcttcgcc ggaggcaaac
    59281 agccaattgc tggacagcgc gatcgtcaat cggcttccat cctgcttcac cgtgatcgcc
    59341 ttctgcccgc catcgctcgg cagaaccttg aggatctcct ccttcatggc tgcaaagctg
    59401 gcctcgcgcg aagtggactg gctcggactt gttggggtcg cgcttcctgt gcggccggca
    59461 tccttctccg aggatccggc gtttttgttg gaagtcgccc ccgtttcccg tggggccgtc
    59521 gccgcttggg cacccttttg gcgtggcgaa agatcccgac tcttttcatt caggcgttgt
    59581 tcaagtgcag cgatccggtg ccgggcctgg taaatctctg cgatcatcgc attatattgc
    59641 gggaccagct tgtcccgttc catgaggcgc gagcgaaccg tttcgaaggc ttgctctcgg
    59701 tctctcagct gctgatcgag cgcactgatg cgcgctttga gggaatcaat cgttgccgtg
    59761 gctgtttgta attgggcggc gagactgtcc cgttcggcat tttcagactt aatcgtgcgg
    59821 agatcgtgat cctgccgagc gacctgggct tccagatcca tgatgcggcg gcgggcgact
    59881 tcgagatcgg aaattgctgt ggagcggcct tgaagagcaa ccagatcttt ttctcgtgtt
    59941 gccagttgct gttccaagct tccgatgcgc tgacgggctt gcagtaattc attattggcc
    60001 atggccaact gactggtgag tttgtcgcgt tcaggagaga gtcggcgcag ctctgctaat
    60061 tccgcatctt tggcgttcag caactggtca tacgaagccg cggccggttg gttctggcga
    60121 tcccccaatt tgctttcaag atcgcgaata cgatcgtgga attccccgag agtcgtcagg
    60181 agctgttcgc gctcctgttg aagggctagg agtttggcat cgcctacctg gggtggggct
    60241 gcgaccggct gtggctgagg cacggtctca caggccgaag caagggcacc gagaaccatg
    60301 agcgcccaca cagctcgtct catacgatct ctccctggtc atcggaacga ctgccgaaca
    60361 tagcggaggg ggcgatagtc gtcacaggac tgttcagatg ggcagcaagc ccagacgacg
    60421 gaggctgatc tggttgatgc tgatcagccg gtgtgcgcac tgcgtgcctg acaaatcggg
    60481 tgacaaccat gcaggcccca agaacacgcc gggtactcta gcgtgttctt gggatggacg
    60541 caaggcgtac aacgcgcaat gagagaggac tactgtccga ccacgatttc gacacgccgg
    60601 tttttctgcc gtccctcggc cgtagaattg cttgcgatgg gtcggctgtc ggcatgacct
    60661 ttggcgctaa tcttctcggc ggccataccg ccttccgtca acgcttggcg tgcgctctcg
    60721 gctcgcgcct gggagagctc ggcgttggtc gggaaccgct ttgccaatcg tcccttgatt
    60781 ggcacgttgt ccgtgtggcc tgccacatgg atggagcgat ccggatagtt cttgagtacg
    60841 ctgccgatac gattcaagac atccgctccg cctggtttca gggtggcctc tccggagtcg
    60901 aacagcaagg tcgtggctaa accgagggta agctgatccc ccagttgttt catcgtcacg
    60961 ttgcccttgg atacctctgc ctggaggagt ttcgacaaat cgtttttcgc ttcggcgaga
    61021 ctggtctttt gttgatcgag tgctccacgg agaccggcca tctcatggtc tcgccgtccg
    61081 atttcatttt cgaggtccga tgctcgctgc ttggcggcgg cgagatccgc cacgagccta
    61141 tcgcgatcac cggccgcacc cttcagcgtc cccagttcct gatccttccc actgagctga
    61201 cgctccaggt cggcaatgtg ctgtttcgcc tgtgccaatt cggacgaaag acgatcacga
    61261 tccccggcat tatgtttgag cccggcaagc tcctggtcct tcgtcgcgag caacccttgc
    61321 aaggcggcca actcggcggc cagccgttcc ttgtcagcgg cgctctgctt caacgccgcc
    61381 agttcttgat ccttggcgga caaccgtcgg tccgcgtcgg acaattggcc ggccaatcga
    61441 tccttgtcgc ccgcggcgtt gcgaagccca gccagttcgc ggtcgcgctg attcagttgg
    61501 ttctccagtt cggtgatccg cgccttggac cgatctgagt cggcggcgct tgatgagcgc
    61561 aggcgatcca actcgccttc gagatcggtt ttctggcgtt ccagttctgc gatccggaga
    61621 tcgcgcggat ccggcttagg cgggatgggc ctggcgacgg ccttcatctc attcagcgct
    61681 cgactcgctg agtcattggg tgccggtgta gatgtgcagg ctgcgagcaa tgcagcgctc
    61741 agcagcacgg gaacgacgca aacgtgtttc atgcaatcct ccttacaaca cagtcagact
    61801 gccggaatgt tcccagcagc actgggcgaa cgccccatgg ttagtgacgt tgattcttca
    61861 ggagatcacg gatctcggtc aacaagactt cttcctttgg tggcgccggt ggggggccgg
    61921 gaggcgtggc cttcttaaag cgattcattt gtttgaccac taaaaagatc acgaaggcca
    61981 gaatcgtaaa atcgagcaga gtctgcagga agacgccata attaatcgtg gcggcaccgg
    62041 cggctttggc cgcggcgagg gatttgcctt tggcctcttc actcaacgga ataaagagac
    62101 tggagaagtc catgtgtccc atgaggagac cgatgggcgg catgagaata tcactgacca
    62161 cggatgacac gattttcccg aatgcccctc cgatgatgac ccccacggcc atgtcgagca
    62221 cattcccttt tacggcaaat tctttgaact cactcatcat gcccattgtg caccctcctt
    62281 atgtgagtcg aatcaatccc gaaaccgtcc cgcatcttat tgttttcgac tggtgtcgaa
    62341 gctgtatccc agcgtgatca ggtagaggtt atcggtatcc gacacacctg gcggcggatt
    62401 cttgttgtac cgcatggtcc attggaatcc gctgaccagc ccgccccaca ctttgaatcg
    62461 caacccggta tcagccgtga gatagagatc tttggaattg gccagcgatt ggaaaccctc
    62521 ctggaagtgg tacaggctga cctgatcgcc gccccacagc ggccaattcc atttgacggc
    62581 ccatcggcca cgggtgctgg acttgtcgtc ggcgagcttg aaatcttcat tgaagtacgc
    62641 ggcgccggct tctgcccaga gcgtcatgtc tttgaagaaa cctgtcaggt caccgcgatc
    62701 gaggagttga tagccgggac cggtcgcgag cgcggtgcgc agtttcaagt cctgaaaggt
    62761 gtcctgctcg aaataggcgg acgcgaacca atacaaccgc ttcgtcagaa agaagtcaag
    62821 cttgatggtg ccgcggctgt tccgcacgat gaggttcccg ctattgtctc cgtagatgta
    62881 acggccgagg atcgtcagcc gcagtgattc gctgcgggca gacaactcgc cgagcaggct
    62941 gccgtttcgc aaatggctgt tgccggtggt ttgggaaaac ccggcttgaa gcgcgcccgt
    63001 ataaatcacg gcaggctgat tcatgcctac aaccgcatcc agcggaatcg ccattggcgt
    63061 tcccatcggc gcggcttgca aggtgagcgt gcccggttcg ccgggctgtg ccgtgccgac
    63121 caccgtggtg ccctctttga gggtgaacgg aatggggcgg ttcacgacca gtttggccac
    63181 gttcggccac atgatcttca cgatgtcgtc tccggcggtc gggccaaatc cagtcttaat
    63241 gtgcaactca tcggcgatca tgccgagaac acgtccatag atgacggtgc cgtctttgag
    63301 cgtgaccgag tccagtgtcg gcgctgcggc agtggtatcc gtgggcgtcg gtgattcggc
    63361 attcacgccg tacgccgtaa gcgctacgat gagcagcgca agaaagaaac atgcatagcg
    63421 tcgcatcatt ccatcctcct ggggcttgcg aatggcaacg gtgtgctggt tgcctgatga
    63481 gtgaaagaca ttttgcgttg tataattgat tctgcgtagg agatcaactt ttctcccgcc
    63541 cattcttccg catggccctg gctcccgccg agtcgcatag atgagatctg ccgcctcccg
    63601 cgctcacgga ctgtccccgg caatctgccg ccatcatgat gtttccgccg accagacggt
    63661 ccaggacccg ttgcgaagaa gcgtccgcac agattatcgc ggagccgcac agtacacatg
    63721 tgtcaagatg agacggcatt gggtgatcgt gacacgccgg cgcatggacg aataggctgt
    63781 atgccgacgt gagcgtagag acaaagcggg actcttcatt tgatgctggt gagtcttcgc
    63841 gaggccttcg cgctctgcac cactcgtttt acatccggtg cgcgctctgt tgacgggacg
    63901 gagagcagcc cttccaacaa cgccaggcgg agcagttgcg cggcgttcgt ggcccggaac
    63961 ttcctcaaca agttggcgcg atgagattcc acggttttct gtgcgatctg cagtcgggcc
    64021 gcaatttcct gactggtcaa tcctgcccaa atgtaattca gtatctcttg ctcgcgcggc
    64081 gtcggttggt ccggccggtt ggttgaaaac ggaaatacct tatccatcgt tgcttctcat
    64141 gcccctttct gatcgtcctg cccggtgatc tcacggaatt gcacgcagtg gtttgtccgg
    64201 tggtttttac aagctccgtg cctgaacttg cggaatgtgc gtcaggatga gccattgcgc
    64261 tggatcagca tgcagccggc gcgatgcgag ggaccgtggc gataggatga gacacatcac
    64321 atcgtctttc attgtctcgc tggagacgat gtgagacagc gtccgcggaa ggtcggacag
    64381 tctaggcggc tagttggccg gcaggtcgag ttcagtcatt cggcggtaga aggtggcgcg
    64441 gctcatgccg agaaggcgtg cggctttggt gcggtttcca cgggcctgtc caagggcgga
    64501 aacgaagcgg ctgcgttcgt cagtcgtgat gggttcaggc gtaagcgctg tcggctcccg
    64561 ctgcagctcc ggcggaagat ccgtcgcttc gagtgagtca cctttgcagt gaatcatcgc
    64621 gcattcgatc gtgctcttga gttctcgcac gttgccgggc cactggtagt ccatcaacgc
    64681 ggccatggtc gccgggcttg cccggtggat gagttttccc atgctggctc gcccttcagc
    64741 gaggaaagag gtcaccagca gcgggatatc ttcctttcgc tccctcagcg ggggaaggtg
    64801 aatgcgagcg acccgaatgc ggtagagaag atcggcgcga aaggttccct tggccacgtc
    64861 ctggctgaga ttgtggtgcg tcgccgtcag gacgcgcacg ttaatttttc tcggcttggt
    64921 ctcgccaagc cgggtcactt ccttttcctg cagcactcgc agcagattgg tctgcaccgt
    64981 gtggggaatg tcgccgatct catcgagaaa cagcactccc ccctgtgcgg cttcgaagag
    65041 cccctgctgg tcatcgatcg cgccggtaaa ggcccctttt ttgtgaccga agagctggct
    65101 gccgagcaag gaatccgtca gccccgcgca attagccgcg ataaaggggc ccgtgcgtcg
    65161 ggcactggct tggtgcaagg cgcgggcgac cagttctttt cccgtcccgg tctcaccttc
    65221 gatcagaacg gtcgaatcca cgcgagccag gtcttggagc tgttcataga tctgggtcat
    65281 gccgcgactc ttgccgatca aatcgtgata gtgcgctttg agttccagca tacgccgaag
    65341 gtgatagacg tccgtcatgt catggagaaa gacgatcttg ctccgtctgt cccgcggatc
    65401 gtcgtgcagc tctaggtcga gccacaggcg cctccctgcc ggcgtgtcga tgtggcagca
    65461 aacccgttcc cgttgcggga tcggatgctc aagaagcgac tgcaccgtca gtcgatcggc
    65521 ctttgtgact ggaaggaggg tctcccaccg tagatcaggc ttgatgctgg ctgagggccg
    65581 ctccaacaga ctggcggcag atgcactcac aaagcgcaca tgtccttcct ggtcgatgag
    65641 cagcgtggcc agccccaggt cctggaggat agccgcgaca tcgtcccgcg cccgttccat
    65701 gtccaccagt tgcatggtca gatcctgttg cgtgcgttgg tgtccgcgct cctgctcatg
    65761 ctgttgaagt gcctgctcgc gcgcccgctg gagcatagcg gcctgttgtt cataggccgc
    65821 agtgctgtgc tgtatgacca acagacggct ggttccttcg cggagggcca atgcttcaaa
    65881 gtgccagggg acgtcgaagg cggtctgttc agtccagaag ccagagcggc cgatgtgcgc
    65941 gtcgccggtc tgccacacct ctgccgcgtc agccagaaaa ttatgcagca cgggggtgcg
    66001 actgtcgacg gagagctgaa gactcgcttc agcctccgga tagagacgaa acagccaggg
    66061 tgatggatgg ccgaggatgc ggaattgcgt gccggcaaca tgttcgagga cgacgtactc
    66121 caatcgctcg aataacgagc acaggaagtc cggcgtgcgg agtgacggat caggagccat
    66181 ggggcctccc ggctgctgcc gaagagttcg atggtgtgag catggcgcga gcgagtgtgg
    66241 tgaatgcttc ttccacgcct tgcccggttt tggcgctgct catcacgact gaccatcctc
    66301 gttgcgccaa ctgggcaagg tcttgatcag tgatatccca ctccgctctc cggtcgtgct
    66361 tattgatcag gacgacgaac ggtacggggc cgagtgtatc ggatgcccgt tcctggacgt
    66421 gcaaggcaat gtccagcgtc gcccgccgca tgccgtcaag gacaaggaga taccctgagg
    66481 acccccgcag ataggacgag cgtagctttt gaaattcatc ttctccgtaa agatcccaca
    66541 ggaccatggt gagagatgtc ccgtcgaccg tcatcgattt tttgtcgact gtgacgccga
    66601 tggttgtctg gtactggtcg gaaaagcagc tgctgacaaa acgccgaacc agactggttt
    66661 tccccacggc aaacgcgccc aacatacaga tctttttctc aatcatgcgg agagtctcac
    66721 ggcatcggct gaatggtgat ttccagtgag gctcgccgat gctgcggatg ggcgacggct
    66781 ggggtcgacc gctgcaattc agcctcttgg gccatgcccc ttgtgtgaaa cgtgagtgcg
    66841 ggaaaggatg cggggtggat cgcatcgagg acagctttgg cgcgaccgtc actgaggcgt
    66901 cgattctggc tcgtcccgcc tacgacatcg gtttgtccga cgatggacac cgtcacggcg
    66961 gcgctggcaa ggcgggccag gtcctccagc tgccgaagca tcccgacgat gcggcgggct
    67021 tgttgaagtt gttccggcga tacgagattg ctgctcccct gttcaaataa aattgacgtc
    67081 tcggtgaatt gctgacgcaa catgtcgaga gactgactca cgaggcgtcg atcgtcatag
    67141 ccatcgaggc cgggtatcgc gcgagcgact gagcggcttt gcctgatcca atccgccgaa
    67201 gccgtgcctc cggcagtcag atggcttcct tccagggcca agtgaaccgt cggtggtggt
    67261 gagagtgcga atcccgcgcg tttcagaatg aacgacgtgt cgagggcgta gtacggttgc
    67321 cacttcgcca ccactcgact ggtgtcgatg ccgctgtctt gcatgaggat agccgggtcc
    67381 gccgcgaggg ggtcacgaag gccggtgagc cgatagcgac cgccttccgg ctcagcgtcg
    67441 gtgacgacaa gtccaggctc cgtggccagg cgagacaggt agctctgcca gcgttggcgc
    67501 tcctggtagg ttgacagggc ccaccaggtc gtgacgagga gcgcgccaat gatcaacacc
    67561 catgtcatcg gtgcgatgcc gcgccggcgt gattcgaact gggcgcgcaa acactcttcc
    67621 aatagtggtg tgacactggc gaacgtggcg gcatctccgg tgaaccgggc caaggcatcc
    67681 gcccgttcgg cgtgaatacg ggtgagcgtg tcttgaagat ggaggcgaag gctttccggt
    67741 ggagtgccgc gaatcaccgc ggcgagaata gccgccggcc cctgttcgat ccagacggtg
    67801 agctcaccga cctgcaacgt attcagcacc tgttccgggt cggcgccgaa tgaatcctgc
    67861 acgaagctcc ggatggccga cagcataccg gacaccaggg tctggtcctg cacggccact
    67921 gcgtcgccgg cggcatgcgc cagcagcagt ccggtctgtg agtgaatgag aaacacctgt
    67981 tcaacccgat agcgcaaggt atgcagaaga acgatttctg cgaacggctt gccggtgcgc
    68041 agcgcttcca ctctccattt cacactttgg atcgacaggc tatgttcgat ggtttgattg
    68101 agcgattgga ccatgccgcg taaggcattg gcgatggcct ggcgaatggc cggcatcatg
    68161 atcggagcga tggcatccac gatcatatga ggctgtctcc gcacggacgc gcccaacgct
    68221 tccgatacgt gcggagttaa gacatgtgtg agctggtggt caccctctcc gcgtagcgcg
    68281 atcgcatccg gcagcacgcg gctgaggttc ttcgcattga ggtcgatatg ctcgacctgt
    68341 tcctggagct gctccaaacg cgtctgttcc ggcgccagca gaaggctgcg tagttcagca
    68401 tagtcgtgtt ctgcaggggg agtgctgcgg ttaggtggct gtgccatacc ggttcctctg
    68461 tcgagcgcga tgagggaggg gcacagtgga gtacatgtga cgatgaacgc tgcggctctg
    68521 tgtgaggagg gcaggccggg accacgcagg catgaaacct ggattatgcc tgttgtggag
    68581 gctgatcatg cgagagcctc tgcgagagtt cctggaacaa ggtcgccagc gatgtccggt
    68641 cggtcttctg atgggaaagt tgttgcacgg cctgatcgag tgtcgccgac agggtcgcat
    68701 gttgttgcgt gaactcaccc gtcagtgagg cggcctgatg ctgcagctgg tctcgcagat
    68761 cgcggtctgc ctgttcgctc tgttctctca actgcgtggc cgtttgctgg agaaccccgg
    68821 cgagggtgtg aagctctgcg gtcagcgcac tgaccgcctt tgtgcgaccc tgctcctccg
    68881 cgttcagccg ccccgtcact tgttccacct ctgtacggat gtattgttcc agcgacgcga
    68941 aacggtcttt gagatcatta cgtagttggg cggcctctgc gagcaattgc gtttcgagct
    69001 tggtaaatcg gcgctcgtga tcgcgcacct gggccccgaa gaggatgtcg cggatcttgt
    69061 cgagattatt accggcggtg ggatcgtcgg cgagggcggt ttccgtctcc gtctcggcga
    69121 gcgtggtggg ggtgcgttcc gaacgtcggg tgcgatcgag atctgtttgc ctgctcatgg
    69181 tgtcgtctcc agggtcgcag tcgtccgtca aggaaagggg gggtacacta gcatatttac
    69241 gcaggtgcaa caagccacgc ggggggcatc accagtggac cagatagggc cgatgatagg
    69301 gtcgtgtgct gttcgattag gaagggttca gcaggcgcac ttcacgttgc ggaaagggaa
    69361 tatccacgcc atctgcgcgc aatcgctgga tgatggcctg gttgagttcg atatgtgctg
    69421 aaccgacgga agcgacaggg acccagggcc gaatggacag ggtaatagag gagtcggcga
    69481 aggcggcaat cccgatcatc gggacaggat cttgaaggac ttttggatgt ctggagacga
    69541 gctcgcgcaa cacgcggagt gtggtctcga tatcggtttg ataggacact ccaatggaga
    69601 ggtccaactg ccggatggtt ccgaagttgt gcagaatttc tccgacgact ttgcggttcg
    69661 gaatgacgat tctggaatgg tcgggatgca gcagagtggt cgtgaagata tcgatcttcg
    69721 cgacatctcc atggaccccc aagagggaga tatgttcgcc gacccggtag ggctttgtga
    69781 agataatcga aagccccgct accacattgc tcagtacccc ttgcaaagcc agtccgacac
    69841 cgagaccggc gacgccgagg cccgccacca gcggcgcgat ttgcacgccg agttgatcca
    69901 gcgcaatgag aaccccgagg ataaccagca gggctttcag gagtcgcagg agcagcaggc
    69961 ggaggggcgg ctcgaggtcc tgttcgttca gccagcgctc gaagagacgg cccaccgaat
    70021 tggccacgaa aagggcggcg acaatgacga gaatcgcccc catgatgcga aatccgtact
    70081 gcaccacgta ttgagtaatc aaattggcga gtgacatgtt tcctccgccc cctcctaccg
    70141 cccgaacaat cccttgagaa gatctttgcc ttgcttcttg aggtcctccg gcttggccga
    70201 ccccttcagg agatctccca ccgcgccctt gaccttttct ttcacctgct cctggacttt
    70261 ccctgcgaac atttttgtgt cgagtccata cgaaggggct tgagtgctgc cggtaattac
    70321 cagtggaagc gagaggcgtc cattggtcat ggcgaccttg gcaatgggag agccggcggc
    70381 gattttctgg ctcagggcct gagacagatt cagattcagt ttcatgtcga gtgattggtc
    70441 gaggccgatc gtgccgccgc cggtcgcctg gaaatcatgg ctgtcgatca agacgcgctg
    70501 taggccgatc agtccttgtt tgatcaccac gtcgctttcg atggtggaga acgccgtggc
    70561 tttgacattg tccaacgaga cgccgaccac tttgagcaac atcgcggcct gttgcaataa
    70621 attgatcccc tcgattttgc cctctctgac ggacatgcgg ccggttcccg ccagggcttt
    70681 cacgagatcc ggatgagtaa agccacgccc gctgagcgcc agttcggcgt tggcggtgcc
    70741 gctgagcgag acctggtctg tgccgacagc ctgcagcgcc ggtccgactt gtagcccttg
    70801 tagtgcgacc gtgccggtaa atggcggagt gggagttccc aggcctaacg ttccctgcgt
    70861 gcgcaggaag cctccgaaga cttggagcgt gaggttggtg agtcgcgcct cttgcccctt
    70921 catttcggcc gccgcctgga ggtctttgat ctctacgggc tttttcaagg gcaatgccac
    70981 aggcaaatcg gcgctgttca cgagttttga ggtcgccgtc accttcgcca gtccgccgag
    71041 caccgatccc ttgacctcga cccgtgagct tcccaatgcg atcgtcaggc ccaggttcgg
    71101 cacctctgcg agctcaaggg gcgcggtgcc ttctttgggc ggatacttcg cccgaacgtt
    71161 catgtgcagg tctttcactt cgactggttt ggctagtggg acggccactg gaaggtcgga
    71221 ggaattgata ctggccgacg ccgcggtgat attggcgagg cctttggttg ccgtgccctt
    71281 gatgttgatg gctgagccac ccatcaccag ggttaggccc aagtccgtca ggtctacctg
    71341 gctggcgggc ggggtatcag gcgggatcgg gtagagagca tggagcgtga gatgcagatc
    71401 cttgatctgg acaggctttg tgagcggcaa tgcgatggga agatccgccg tatcgatttg
    71461 caacgcatta atcgtggcat ccaggttgcc gccgactgcg cgtcctttga gccccactgc
    71521 gaccttgcca aggccgaggt tgaacgagaa ggatttcaca tccagcgttt caaccagtgg
    71581 tccgaatgtc ccatccagat gcacgggaag attatagggc aggacggttg ctcccaggtg
    71641 caccgtggga ctatcgccca gatggaccga cgtcagcaaa aattccagct gggtgacggt
    71701 gtactccgtc ggcttcgcgg tggactcgtc acgatacgtc agcttcccgc catcgatcga
    71761 aacccggtcg acggcaaaaa gcgccagcgc ttggagtggg ctgccggccg gttgtcctgg
    71821 ggcctcgggc tttgagggcg cggcaggaga cgtgggtttc gcgccgatgg tagagatgtt
    71881 gagctgcccc tgcgaattct tcaggaccag aatgaccggg tcgcgcaggg tgatctcttc
    71941 cacatcgact ctgcctttga gcagcggcag gagcttgacg cccacatcca gcgacgcgag
    72001 agaggcaaag ggccccgtgc ggaacgccgg gtcatcctgg acaacaaacc cgccgattcg
    72061 agccccgatg cgtggccaaa tggtcagacg gatatcctgc agttcgactt tccggttgag
    72121 cgcctcctca atcaaaggcc ggtaccgatc ctgatacttg ttcaaatcga tgaggaaagg
    72181 caaagcgatg atcaggatca gaaagagcag gatcacgaca ccgatcccga ccagaatttt
    72241 catcacgtac ctccgctgaa atggtcccgc agtataggaa tgcgcgcgaa atcggtcaat
    72301 ggcggcaaat cggccttgcc tcgcacgacg tcgcgatgga aaacccccgt cgtggtctct
    72361 ccgccgtgcc gtttcgagat cgagtctgag gtaagggacg tggcgtccct taccttgccg
    72421 ctcaccggcc tgcgtgataa gatgccaccc tttgaagtcg tccgatcgcg gagaggtgcg
    72481 agagtggacg aatcgacatg cttggaaagc atgcgtctcg gcaacgggac cgtgggttcg
    72541 aatcccaccc tctccgccat cgaaagtgcg aagtaagatt gggaaggcgt gcgagtcgaa
    72601 tgaccattgc cggctgaggc ggtgggattc gaacaagggg gaatcgcggg ggagggactc
    72661 ccctgcgtgg ggagacacga gggggcaggc cccctcagtg ggagcgcgta tgcgtgactt
    72721 cgaatgccca ccctctccgc catcgattag agtcgtgtca cgtcgggcat gacgggaacc
    72781 ggtatgagaa gggggagtca tacaaggctc tgccctttgc ctttcatccc tgccggatca
    72841 cgaaatcctc ggggccgggc cctgtgcaac agaaccctgt gaaccccgcc aggtccggaa
    72901 ggaagcaacg gtaagcggtt cgttctgtgt gccgcaggat cacctggccc cactttttta
    72961 gacgactgag ccacaagtgg aagaatgtct gctctggcgt cccgaactgc cactcgacac
    73021 acaatcgtca ctactagtta gagcctgacg aaattggatt accaagtctc tgcaagaaaa
    73081 taccggcctg gaacgtttga tgatgtcatc gggcagtcac acgtcgttca gacgttgatg
    73141 aattcgatcg ccaccaagcg aatcgcccac gcctttctct tttcgggcac gcgtggcgtc
    73201 ggaaaaacga cggtcgcgcg gatcctggcg aaggcactca actgcgagca gggtccgacc
    73261 ggttccccgt gtaacacctg tgcgaattgt caggaaatca cgcagggtac gtcggtagat
    73321 gtcgtcgaaa tcgacggcgc gtcgaatacg agcgtggacg acgtgcggga aattcgcgag
    73381 aatgtcaagt tcaccccgtt ccgaggacag tatcgtgtct acatcatcga tgaagtgcac
    73441 atgctctcca actcggcgtt caacgctttg ctcaaaactt tggaagagcc tccgtcccac
    73501 gtcgtgttca tcttcgcgac gacggaaatt cacaagatcc ccgcgacgat tctctcgcgc
    73561 tgtcaacatt acaactttcg ccggatttca aaggctgaga ttgtgcagcg gctgcggcat
    73621 gtggcggacc aggatggctt gaccatcgaa gatcgaagct tgatggccct ggcgcgtgcc
    73681 agcgaaggta gcatgcgcga tggcctcagc ttgctggacc aagtgattgc gttcggtggc
    73741 aagacgattc ggcatcacga tcttgaaacc ctgctcggcg cggttccgca ggagcgggtg
    73801 cgggcgatgg ttgaggccgt gatcgaccag aacagcgcca aagccttgca ggtcatcgcc
    73861 ggactcttgg atcaaggcca cgacgtccgc gcctactgcg ccgatctggt ggagtatgtg
    73921 cggaatatgc tggtcgctgc cgtagtgcct gcaggtccgg agttgcgggg actgatcgaa
    73981 gtgaccgatg aagacctgac gcaactggct cgggacgcag aacagttttc ggtggagcac
    74041 ctccaggaat tatttcgatt gtgggcggcg gcagaggatc atctgcgggt cagcgcccat
    74101 ccccggtttg tgctggaaac ggctgcggtt cgtgcgactc gtctcctgcg cgcgcctgaa
    74161 tccccggcgg ggttgccggg agtgaatgtt cagtcaagtg caacgcctgt cgatcgggca
    74221 acgggaactc gcacatcgat gtcatcatcg gagaaggtgg tgtcacccaa acccacgaaa
    74281 cgtgatacgg tgaaaagcag ctctgacgcc ggagacaaac cggttgttcc gagcgcatcg
    74341 gctccggcgc cggctcctgt tgcgcgtcct cgtgaagcgg tggttccatc cagtcagact
    74401 cctgctcctt ccattccatc tgcgcaggcc tccgcgaccg cgcaggttcc ggtcgagtcg
    74461 gcgtcgccaa aacccgaacc cccgtctcac agggcaaccg cctcaccctc ggcagagatt
    74521 aattgggagg agtttcaaga ggccgtttcg ggtcagcatc ccaatattgc gccgtttttg
    74581 gaaatgggcc gggtagtgaa gattgaaggt ggattggtta cgttgggctt taccaaacag
    74641 gcgacggtgg cgcgagccat gctggagaag gaagacaatc ttcagtccct ggcggcgttg
    74701 ggagaacgaa tatacggaac tgccttgcgt gtccgaatta tcgaagtggc ggagcaggat
    74761 ccggaggcca cccccacgat gaagcaatta cgggttgcga aggagcagga gcagcgcgtc
    74821 atcctgacac aacaggcgaa ggcgcatcct ctcgttaaac aagcgctgga gatgttcggc
    74881 ggagagttgg ccgaggttcg cacgacaact ccggcacagg aggtacagga atgaaaagtc
    74941 cattcgggaa tatgtccaac attttgaaac aagcacaggc catgcaggaa caaatggcta
    75001 aggtgcagga gcaggctgcg accaagacgg tatccgggac cgcgggtggg ggcattgtga
    75061 ctgtgaccgt gaacggcgca atggatctgc tgagcgtgaa gatcgatccg gaagtcgtga
    75121 agtctggtga tgtcgatatg ttgcaggatc ttgttgttgc ggcgggtaac gatgccttga
    75181 agaaatcacg cgatatgatg gctgaggaaa tgaaagccgt cacgggtgga atgaagattc
    75241 ctggtctgtt ctgatgcgat cgagattgtg ttgtgtcgaa tgcgggaggc tatgggcctc
    75301 tcgtgtgtgt ggcctggatg tgacgatgcg ctatgagtat tgatcagcag ggactgttgg
    75361 caaaattggt tcgtgagttg gtgcgtttgc ccggaatcgg tcagaaaagc gcgcaacggc
    75421 tggcgttcca cctgctcaag gcggagcggg aagatgcgtt gcgcttggcc gaagccattc
    75481 aagccgtgaa ggacggactc tcgttttgcc gccaatgtcg caatatcgct gagggggagc
    75541 tctgtgagtt ctgccgggat tctaagcggg atcgcagcaa gattctcgtg gtggaagagc
    75601 cgagcaccct gtatgcgata gagcgcgccg gtgggtaccg gggtttgtac cacgtcttgc
    75661 tcggagtctt atcgccattg gatggcgtcg ggccatccga tattcgcgcg gaggaattgc
    75721 ttgagcgggt caaagccgga ggtgtcgaag aagtcatcgt ggccaccaat cccacgattg
    75781 aaggtgaggc gacggcgatc tacctgacgc gactcttgaa gcctcaccac gtccgggtca
    75841 cgcggattgc ctatggaatc cccgttggga tggacatcga gtatgcggat gaggtgacgc
    75901 tggtgaaatc gatcgaaggc cgacgagatt tgtgacgcag acgtcccgct gcctttgaga
    75961 gtgcccagtt gcggataatc ctcatcctcg cccccacccc agacgaaccg attgacccct
    76021 ccgatttgcg gctgctatag tcggaactcg ttgggcggtc tggcggcgat accggcctag
    76081 taaaccgagt attcaatgaa gacttcggcg aagaaatcca ctcccacccg aaagaaacct
    76141 gcgaaggcga acggggttaa gtatcctgat attcagaaag acctcgaggg ccaacgtgcc
    76201 gctatccttg ccgaagccgg agtggtgctg acgaacccta ccggtctgga agtgtttccg
    76261 gatgtgagcg accaagcctc cgccgaagcg gatcagcatt tctcatttcg cattcgcgaa
    76321 cgggaacgga aactccttaa gaagatcgat gaagctttgg ggcggcttgc cacgcatacc
    76381 tacgggatct gcgaaggttg ccagggcgat attccctata agcgactgaa ggcgcgtccg
    76441 gtcacgacct tgtgcatcga atgcaagacc agtcaggaag aagcagagaa gtctccccgc
    76501 tgattcggtt ctgtcccctt cgaattcctt ttgtgacacg caggattttg ccgcacagca
    76561 cccgtttcat tactggtgtt tgagggcttt gacgcgtgat tgcgccaggg ctgagcgatc
    76621 cgcttcttcc ggaatgccct cagtaagcgc cagatacgtc tcatattcat tgatagcgcg
    76681 tctggggtta gcctccgcaa tggcctcggc caggttgaac cgggcaatgg cgtaattcgg
    76741 gcgcatcagc acagcttttt cgaaggacgc gagcgcgttc tgcttctcgc ccagcttgcc
    76801 atacacatag cccagattat tgacgacctc cggggagttg ggacgttgtg ccagtgaggc
    76861 gagcagttcg cgcttggcct tttccaagtc gccggcagct tcccaggata cacccagccg
    76921 gtgatgcagt tccgcttgcc agacctggct ggtgaatcct gaagcgcttg ccttctggta
    76981 ggcatcgagc gcgatggtct gatttttcgc tccacagtat gcgagttgtg cggtttggcc
    77041 tcgggcgaat tgttggagcg aggcgaaagg ctccttgtct tccgcgccct gcgtgtcgag
    77101 gtgttggcca ccgctttgct gtcccgtatc ccgcagccga tcgagaaact ctttccggta
    77161 gggagagaag agcttcacgt acggatcaat ggaaaaggac gcagccagaa gctgcggatt
    77221 ctttcgatcg cccaggacga gataccgggt ggtggtcttc tcatcgcccg tgtcatagag
    77281 ggtgctggag gccatattct gacgatccgc caattgagcg acggtcaggt agaactccag
    77341 gctgggtttc agttgggtca gggtatagcg aagcgttttg tacggaccca agtcgagtcc
    77401 ggaccgaaag atgaacagcg tcccgaccag tgtcccgtct tcatcgaaga agtacgactc
    77461 ttcgcccttg gacaggctct gttcggccga aaagcgcagc tcttgcccac tgccccacgc
    77521 ttgggttttc ggaacgatgc caggatggtg ctcttggaac accttgcggg tatcgcaaag
    77581 tttcgtggat ttcaggaggt cgaggtcact ttccgatggc ggaagcggcg cacgggctgg
    77641 cggcggagtt tcgcaccctg tcagcagggc gaacatggtg agcagtgcag gcagcagtga
    77701 gagccgacgg tgcatggccg gaatcgatgc cccaggtcaa tgccatatag gatcgaggaa
    77761 tagagcatac ctgaatggct gtagagatgc gactggcagg cggagtaatg acaggcttct
    77821 cctcgccgcc gagaaaagat cccggcaggc gaggggtgga gcaggttacc gacgttgcga
    77881 gtcgatcata gaatcttcgg ccgtgtagcc gaagaggtac ggcgcatgtc tcgcaatggc
    77941 gtagagtggc cgattcaccg tgtagtctac gagatgcccg accgggtgga gcacaaagcc
    78001 cagccatcgg taaggattgt cgttcgcctg tgaggcggcc atgggatcgg aatagaccag
    78061 ggaggtgctg tccatcgcgc tatagatgct gagcggcgcg ttgtagttct ggtcctgcgt
    78121 cgccacctga ttggtggtgg agcagcccgt ggtcagaagc agtcccaaca tgattgtggc
    78181 caaagtcccg cgcataccgt gcctctcttt cttctatagg aacattcgaa gagacgaagt
    78241 ctgttccttg tgagaaaagt ctagcgcagg gtcctgattc tgtccagttg gacggcaccc
    78301 taggtcgtcg ttcaagctgt ctcagctgat cgctgtggca attgacataa aatatgagca
    78361 gtttcaagta gttgagagat tcattcgtcc gtagtgatta catgcggttt cgagtgcgag
    78421 gtcatcgttc caaatgaatc ctgggagatg gcctgtcctc tcgtgtaact gtcgggctta
    78481 tcctcatgtt ctaagggatt ccaaagtcgt cttgcatcaa gaagctactt tttgtagtcc
    78541 tcgttgctct gattaagtgc attcatcgcc cctccgccgg caagcgccta atcgattgcg
    78601 cacaagtcgc cgatgatgac gcaacgctat gcgcatcatt acccggaaag cctgcgagat
    78661 ggagtggaga ttttggaccg acggtcacac cgtgacacaa aaatgaccac agtgttgtag
    78721 ggattgagag tgttattcgg aaggggttga aaaattggtg ggcgatacag gtatcgaacc
    78781 tgtggcctct tccgtgtgaa ggaagcgctc tcccactgag ctaatcgccc gagcaggacg
    78841 ggagtctacc acggtccggc aaacaggagc aaccaactgc ccgacggcca taggtcctca
    78901 cgcagggagc tctcagctcg tccgccaagg ctaaatcgtc cttggttttc ttgacatcga
    78961 gaaaaggcga ttcttacaat gagcctcctt ccatcttttc aaaggtttcc cattcatgcg
    79021 tgaagacatc gtcatcatcg gtggcggttt ggccggctca gaagcggcgt ggcaggcggc
    79081 acatcgcgga gccaaggtga cgctctatga gatgcgtccg aaagagacga cgaaggcgca
    79141 caaaaccggc gatctggccg aacttgtctg ttccaactcg ctgggatcga tggatccgct
    79201 caatgcgccc ggcattctca agggcgagat gcgtcgcctg aactccctgg tgattcgtgc
    79261 ggccgaggag gcacgcgtgc cggccggttc cgcgctggcg gtggatcggg aaatcttcgc
    79321 acgggccatc acgcaagcgc tggaagggca tcccaatatt cggatcattc gcgaagaggt
    79381 gacggaaatc cctgcggatg ccgtcgcgat catcgctacc ggccccttaa cgtcagaaaa
    79441 attgtccaaa gccatcagcg aactcactca cgaacggcac ctgtatttct tcgatgcgat
    79501 ctcacccatc atcgacgcag actcgattaa catggatatt gtgtatcgtg cgtcacggta
    79561 tgggaagggc ggcgcggact atctgaactg tccgatggat gaggcggcct acaatgcgtt
    79621 gtacgacgcg atgatggcgg ccgaaaaagt gcagccgaaa gagtttgaga aagtcgccta
    79681 ctttgaaagc tgtattccga tcgaagtcat ggctgagcgg gggcggcaga ctatgcagtt
    79741 cgggccgctc aaaccggtcg gcttggacga tccgaaaacc ggccggcgcc cttatgcggt
    79801 cgtgcaattg cggaccgaaa acgttcatgg cacctgctac aacatggtcg gctttcagac
    79861 caagctcacc taccccgaac agaagcgagt ctttcggctg attccaggcc tggaacaggc
    79921 cgagtttttg cggtttggca gcctgcatcg caacaccttc atcaactccc cgcagctgtt
    79981 gcgcgacacg ctccagctga aaacccgcgg gaccgtcttt tttgccggcc aactggtcgg
    80041 tgtcgaggga tatacggagt ctgcggcgat gggtgggatt gccggcatca atgcggcacg
    80101 aggtctcgcc ggtcaaccac tcgtgacccc gccgcctacc agcgctcatg gttgtctgat
    80161 ggcgcacatc accaaaagcg atccggcaca ttttcaaccg atgaatacca acttcggcct
    80221 gttcccgccc gtcaccgtca agacccgcga caaagatcag aagcggcgtc tcatccagca
    80281 acgagctgtt gaggattttg acgcatggat cgcgcaatcc gggctttcct agacgtgttg
    80341 gcggttcagg acgacgcctc tccgcaaacg atccgcgcgt atacgtccga cctggctcaa
    80401 ttccgcgcct ttgccctggc tgcgctgaag cctcgtgaca cattaacggt cgaatcgata
    80461 gaagcgggtc tgattcgtga attcctcgcc tcgcgtgatc gcaaggggga gaagaaaact
    80521 tctctcgcca ggaaattagc ctgcctgcga agtttctttc ggtatctggt tcgcgtcggc
    80581 cgcgtgcagg cgaatccggc ggaagatgtg cgcgcgccga aactgccgaa acacctgcct
    80641 caagtgctca cgaaagacga tgccggagcg ctgatggaat ttccgccggg agacgcacgc
    80701 gagggtttgc gcgaccgtgc cattcttgaa actctgtatt ccaccggcgc gcgtgtcagt
    80761 gaattggtcg gcatggatcg gcaggacatc agtcgcagtg aaggtgtcgt gcgtgttcgc
    80821 ggcaagggag ggaaagagcg agtgatcccc ttgagtccac tcgccctgga ggccattgat
    80881 gcgtaccatg cgcaggcgtc cgtcacagct gcttcgaatt cttcggatcg aggggcgcca
    80941 ggcggctcgc ccgtttttcg caatcgccgc ggagggcgat tgacgaccag aacgattgcc
    81001 cgcatcgtgg cgaaatattc gggacaactg agcggtggcg cgattcaccc tcacacgctg
    81061 cgacattctt ttgccacgca tctgttggat gaaggcgcag acctgcgggc tattcaagaa
    81121 atgctggggc acgtgtcgct cagtacgact caaaaataca cccatcttgc gacggaccag
    81181 ctactcgcgt tatacgatcg cactcacccg cgcgcgggcg ctgcgggtga agcggaacaa
    81241 gggaataagc agaagggttc gcgatgaaga ttcgatcgac gacagtcctc tgtgtcagac
    81301 gaggcaatca ggtgacgatg gggtgcgacg gacaggtcac ggtcggtacc accgtcatga
    81361 aacataacgc caggaaaatg cgccggatgc ataacgacac ggtcttgtcg ggattcgccg
    81421 gtgccacggc cgatgccttc accctctttg aaaaattcga ggcaaaactg gcggagtatc
    81481 gcggcaacct gacgagagcc gccgtggaat tagctaaaga ctggcggacg gaccgcgtgt
    81541 tacggcgtct tgaagcgctg ctggccgtgg ccgatctcga tcactccttt attatttccg
    81601 gaacgggaga tgtggtcgag ccggaggacg gtattctcgc catcggatcg ggcggaccct
    81661 acgccctggc ttccgcgcga gccctgctcg cgcactccga actaggcgcc gagcccatcg
    81721 tgaaggaatc gctgatgatt gccggcggga tcgacattta taccaaccaa cagattgtga
    81781 tcgaatcgct cacgcgttag gatctgccct gtcatgacca ccgactccac gtcccgttcc
    81841 ctcaatgtga ataacctgac gccccggcag atcgtcgagg ccctggatcg atatgtcatc
    81901 ggccagcagg atgccaaacg gatggtcgca attgccttac gcaatcgatg gcgccgtcag
    81961 caactggccc ccgagttgcg tgatgaagtc atgccgaaga acatcatcat gatcggcccc
    82021 accggtgtcg gcaaaacaga aatcgccagg cggctggcga aacttgccga agccccgttc
    82081 atcaaggtgg aagcctcaaa gttcaccgaa gtcggctacg tgggccggga tgtcgaatcc
    82141 atcattcggg atctcactga gcaagccatt agtctggtga agacgaaaca tctggaggat
    82201 gtgcaggaga aggcttcacg gctcggggaa gaacggctgc tcgatttgct gttacctggc
    82261 gggccatccc gtccggcctc ggcaggattc gaccatgccg ggtcgcgagc cgaggccgca
    82321 gagccgactg agtctccgga tgccacgcgc tccaagctac gactgcaatt gcgcgacggc
    82381 aaattggatc aacgatcagt agaagtcgag gtcaaggaac gggccttacc actcggtgtg
    82441 atctcgaatc agggcgggat ggaagacctc gaagggaatc ttcgcgacat gctgggcggc
    82501 atgttccagg gcaagaaaaa gaagcgggtg atgaaagttc ccgatgccct gaagcatctg
    82561 actcaggagg aagcgcagaa actgatcgat atggacgagg tggtgcggga agccattacc
    82621 aaagtagagc agaccggcat cgtcttttta gacgagatcg acaaaatcgc cggccgtgag
    82681 cgcgcgatgg gcccggatgt ttcccgtgaa ggcgtacaac gcgacctcct gccgattgtg
    82741 gaaggatcta ccgtcagcac gaaacacggt gccgtccaga ccgaccatat tctgttcatc
    82801 gccgcggggg cgttccacgt agccaaacca tcggatctca ttcccgagct gcagggccgg
    82861 tttcctatcc gtgtggagct ggcgccgctc accaaagaag acttcgtccg tatcctgacc
    82921 gaaccgcggg gcgcgttagt ccgccagtat cacgcgttga tggccacgga agggctcacc
    82981 gtcgagttta cggacgatgg cttggcggaa attgcggcga cggcggtgca ggtcaacgag
    83041 cgcacagaaa atatcggcgc ccggcggctg tttacgatca tggaacggct gctcgaacag
    83101 gtgtcgttcg aaggctcgga attggaacag aaggcggtgg ttgtgaatgc cgcgtatgtg
    83161 cgcgagcgtc tgctgaatat tgtgaaggat caggacctga gccggtatat tttgtaagcg
    83221 ggactagcgc gagcgaggag tgacgcatga acaaactgat taagaaagcc gacgtgctga
    83281 ttgaggcctt gccctacatt cggacgttca agggcaagac ggtcgtcatc aagtacgggg
    83341 ggcatgcgat gacagatgcc tcgctcaaag cgcggttcgc gcagaatgtc gtgttgctga
    83401 agtacgtcgg cttgaaccct gtcgtggtgc acggcggtgg cccgcaaatc gatcaaatgt
    83461 tggatcggtt gggcatggag gcgaagttca agcacggcgt gcgtgtgacc gatgccgcga
    83521 ccatggaaat cgtcgagatg gtccttgccg gccgtatcaa catggaaatt gtcgatctgc
    83581 tcaaccggca cggcggccag gcggtcggac tgagcggcaa agacggcggc ttgatgttga
    83641 ccaagccgtt gacggcgaag gcctgggcgg aaagcatcga aaaggacatc gatgtcgaag
    83701 accgggacgc ggatttcggg tttgtcggag acgtgaagtc gatcgatccc accttactgt
    83761 tgaagctcca ggaagatcac tacattccgg tcattgcacc cattggaacg gatcgggaag
    83821 gcaacaccta caacatcaat gcagacctgg tcgccggggc aatcgccgcc gcgctgaaag
    83881 cggaaaagct cgtgatgttg accgatgtga agggcatccg ggatgccaat ggccgccatc
    83941 tctcgacggt ctcccgcaaa gacgtgcagc gcatggtgaa gcgcggcacc atcagcgaag
    84001 ggatgttgcc caaggtgcat gcctgtctgg atgccttggc cggtggggtg ggaaaagcgc
    84061 atatcgtcga tgggcgggtg ccccatgcca ttctgctgga aatatttact cacaaaggca
    84121 tcggcactga gatcgtttcg taggcagtga accaaccacg cgggtatacc tcaaccaaca
    84181 gcggtgtgaa ggacctctgg ggccgggcgt attggggcca atagggaacg actgcgtcgg
    84241 cgctgagatg aatggttcag tcgccggtat gctaacccgt ctccctcgtc tatgcgcacg
    84301 agccgtatgc cagtgtcata aacatctcag agcgaagcac gtcatcaagg actgagtctg
    84361 gacggagaaa catggcgcgt aaacgtatgt cagtaggccc tgccaccagg cgggcgatca
    84421 aagatatatt ttcgcgactc gactatgcgc agctaggccc gatttattgc gatgaaggcg
    84481 gcgacgagtt ttgggccgcg aagcgagggc cctgtcagcg tctcgggacg aaagtagcca
    84541 cggcgttgaa ggcccggctc aagccgggcg gcacgagtgt gtatgtcggc gcgggcgtgg
    84601 cggagattcc gccgctggtg atggaagcgc tggagctgga tcggcaggtg gtggcctgta
    84661 acttacggcg gcaggaggtg cttcttctga atcgtgcctg cggcgagctc cccatccggt
    84721 ttcagcatgt ggatgcgggt tcggtcaagc agcgggtgga tcatctctgg ctggtcagtg
    84781 tgctgaatga tcctgaacgc tttccggaat tatcggtgct gtcctacggg cgggcgaatc
    84841 ccgtgacctt caatgcgaaa aaatttgtca cgcagcaacg cacggttgcc cgacttgtcg
    84901 atcgttgcct gagccgatta actgtgccgg gtttagtgac caccaccgtt gaagaggcgg
    84961 tgtgggttgc cgagtggtgc catcgacgag gcattcccta ccggattgac gagcgcacgt
    85021 atgcgtcacc cacggtcggt gatcccatct gcctcatgcg gatcggataa ggagcgtggc
    85081 ggctgattgt ggtggcgtcg ttagacccaa aaccccgggc gaggtttatc cggtccggtg
    85141 aggtactgac gggaatatcg aatataattc tgagccgatt cccgtatcca tgcgagttcc
    85201 tgctcggtca ccggacgctt gaccttggcc ggtgatccca gaatgaggct cttcggtgga
    85261 acgatggtgc cttctgtgac gagcgctcct gcgccgacga cggagtcttc cccgatcacc
    85321 gcgccgtcca tgatgatggc gcccattccg accagcaccc ggttctgaac ggtgcagcca
    85381 tgcagcacca catggtgccc gatggtgaca tcatcgccga tgatgagggg atgggtgtca
    85441 tgtgtgacgt gcaacatgca gaggtcctgc acgttggtcc ggttcccgat acgaatggag
    85501 ttcacatccc cgcggataac ggcatggaac caggcgctgc actcttcgcc catcacgacg
    85561 tcgccgatga tgaccgccgt ttcttcgata aagcaggatt gggggatcgt gggcttgatg
    85621 ccctggaagg tccgaatcat ccgctcaatc taggatagct gtagcacgaa tagcaagaga
    85681 gctcgtttga cagacttttc cgctgtctcg tagactgcct cgttatggct tcatcattga
    85741 ttacgccttc acgggcgaaa ggtaagaaac ccaccatcgg cttcgtgaac ctgggttgtt
    85801 ccaaaaacca ggtggactcc gaagtcatgc tcggaacgct ggtggccggg ggattccaac
    85861 tgaccggcga tgcccgtgcc gccgaagtcg tgattatcaa tacctgcggc tttatcgaag
    85921 aggccaagca ggagtcgatc aatagcatta tcgagcatgg ccgtctcaag aagtccggct
    85981 cctgccgggt cttgatcgcc gccggctgtc tggcgcagcg ttatcagggc gagcttttga
    86041 aggaactgcc cgagttggac ggcgtggttg gaacgggcga attcggccgc atcgcagata
    86101 tctgccgcag cctgttggct ccgaaaaaac gccagcaacg tctctggctg gggcagccgc
    86161 cgtatctcta cgacgccgac acgccgcgcg tgcgtctggg gacgccccac agcgcctatc
    86221 tcaaaattgc ggaaggctgt aaccgcaact gcgccttctg tgccattccc atcatgcggg
    86281 gcaagcaacg gagccgaccg attgactcga tcgtggctga agcgagacgg ctggccaaag
    86341 aaggggtcaa ggagctcaac ctcatttccc aggacaccat caattacggg gtggatctcg
    86401 ggctcaaaca gggactcacg gcgttgctac gtgaattggt cacggtggac gatgtgcgat
    86461 ggatccggcc gttctattta tatccccagc aggtcacgga tgagttactg gagctctatg
    86521 ccggcgaggc gcgcattacg aagtatctcg atatgccgct gcaacacatc agtgacggga
    86581 tgctcaagcg catgcatcga ctgggtgatc gcaagcatct gaccaagttg gtggagcgta
    86641 ttcggaccaa aattcctggc gtgttttttc gaacggcgtt tatcgtcggg tttccgggtg
    86701 agacggacaa gcagtttgaa gagctgaagc agttcgtgct cgacatggag ttcgatcgtg
    86761 tcgccgtatt tctttattcc gacgaggaag gcacatcggc cgtggatctc gaccgaaaag
    86821 tcgaccaggc ggtgatggaa gaacggcgca atgagctcct ggccctgcag gaatcgatct
    86881 cagagtccaa aaaccgtgag tatctcggcc gtacgatcga agtcctggtg gatggggtgt
    86941 ctgaagagtc ggaccggctg ctagaggcga ggcaccaggg cttggcccct gagatcgacg
    87001 gggtggtgta ttgtgagcgt gggattgcca ggccgggcga attcatttcg gtcacggtga
    87061 cagatgtcgc cgggtatgat ctgattgccc agcctgctgg tcagacccac cagccaaacc
    87121 gttcttccga ttctcctggt ttgctcatgc ccaaaaaagc cggggcgggt taccgataag
    87181 cccgtgctca ccgggacccc gccccgtcgt tctgcccgca gttaaatctt cgcgctcaga
    87241 tacagggatg cgccgcgccg gttgatcaag accagaacgg tttgtccctt cttgagactc
    87301 gatgcggccc gatcgaactc cttcatggag gtgacgggtt tgcggttgat ttcccggatg
    87361 acgtcaccgg gcatcaagcc ggcacgctct gcatcactct ccggatcgac cgtggtgacc
    87421 accactcctt ggatctttcc cttcaacccg agttcctggg cggtttcccg atccagttcc
    87481 tgcacggcga ggcccgccag cggctgatcc gagggttcag tctctgtctt cgcgacctgc
    87541 tggttctccg gcagttcggc cagcgtcacg gccaactctt tttcctggcc gtcccgcaat
    87601 actgtaaccg tggctttgct gccgaccgca gttcgtgtca cggagcgttg caatgtcacg
    87661 gcatcctcga tcggcgtacc ctggaaggaa aggatcacat cgccctgctt gattccggcc
    87721 ttatccgccg gaccttcttc cttcacatcg gtgacgactg caccattcgt tcccttcacg
    87781 ccaaaggatt tggcgaggtc atggttcaga tcttgaatgc cgatgcccag gtagccgcgc
    87841 acgactttgc cggtcttgac caggctttca taaatcggct tgctcattcc ggtcgagacg
    87901 gcaaacccga cgccttgata tccgccggtc tgggaaaaga tggccgtatt gatgccgatc
    87961 agttctccct tcgtgttcac gagggccccg ccgctatttc cgggattgat ggcggcatcg
    88021 gtttgaatga aatcttcata ctgcgtgatc cccatatgtc cacggcccag cgcactgacg
    88081 atgcccaggg tcactgtcga gttcaggcca aagggatttc caaccgccag tacgtattca
    88141 ccgacttgca gacggctgga atcaccccag ggaacagtcg gaagctgagt gccgtcgatc
    88201 ttgaccacgg caatgtctgt ctttggatca ctgccgacga tgcgcccctt aaattcgcgt
    88261 ttgtcgggta gcgtgaccgt gacggttttt gctcctgcaa tcacatggtt attggtcaac
    88321 acatatccat cctgagtgac gatcaccccg gacccctggc caccacctcg atgttcccgc
    88381 ggttccgtgg gggggccgaa tcgacgcggg ccgaacggtg atccgaagaa gtcgtccatt
    88441 ttcccccgtg gtcgaccgtg gtcagagact tcctccgcgc cgctggtggt gatgttgacg
    88501 acggctggtg tgaccgtctt ggccacatcg gcgaatccga cagcgggcaa ggcgccagtc
    88561 ggcgctatcg ggcgttcctc tacgacgggc gatggattgg aggcgtgaga cggtgaaagc
    88621 ggagtatagc tccagattaa ccccgcgctc acagcggcaa taccggctaa gacactgact
    88681 gacggtgtgg gtcgtttcat atgggctcct cctagatgac ggtgatgtgt acacgcgaga
    88741 gatgaatctt ccgtgtgaca gtcaattgga aggtaacagc cctggatgag aaaactatta
    88801 acgaggaatt agaagtcgtt aagctgctgt ggccgccaac ggcaggacga gagtgaatgt
    88861 ggatcctttg ccgacctcgc tttggacttc gatgtgtccg tggtgggcct cggcaatcca
    88921 ggcgcagatg gccaagccta ggccggtccc ttttttggta tgggcccggg cgtcatccgt
    88981 gcggaagaag cggtcgaaaa tctgcttgtg cgcgtcctgc ggaatgccga tcccgtaatc
    89041 gcgtacggcc aggcgcgcgg tggagccttc tacccgtaaa tcgatttcga ccttgccgca
    89101 ggggtgagaa tatttgaccg cattgtcgac gatgttgagg atgagttccc gcagacgaag
    89161 ttcgtctccc cgcacggtcg ccggctgaat cgtacccatg atgacatcca cttcccgttc
    89221 ctgaccaagc aggcaggcct ggcgttgaat gtcctccact aacgcctcca gccggaccgg
    89281 ctcgcattcc gttttcacct ggcccatatc cgcccgcgag aggaacagca attcctcgac
    89341 gatgcgggac atgcgatcga tttcctccag gttgctttcg agcacggcga tgtagtcttc
    89401 gaccggtcgg ggacggcgca ggactaattc gctctcacct ttcatgaccg tgagcggcgt
    89461 gcggagttcg tgggaggcat cactgctgaa ctggcggatt tgcgcgaagg aggtttccag
    89521 ccgggcgatc atattgttga acgtatcggt gagacggccg atttcatccg ttgaacgttc
    89581 agaatgcagc cgttgcgtca ggtcgccggc cgcaatccgt tgcgcggcaa cggtgatgga
    89641 atcaaccggg cgcaaggcac gtcccgccag aaaccatcca cccaccaaag aaacgaccaa
    89701 ggcgatcggc gccatgacga gcagcacgag gagaaagcgg cgcagcgttt cttcgacgcc
    89761 ttccagcgtc gttcccacct gcacgatata gagcaatgag ccacggtaaa taatgggaac
    89821 ggacaccagg cgcagcggcg gttcattggg atatctggcc gattcgaaga cggtgctgcc
    89881 ggtaaaggcc gcttccaggg ccgggcggct caaggggaca tcgtgttgtc ggatattcgg
    89941 agagcggatc gtgatggtgc cggtggggct gaagatctga aaaaatttat cgatacgagt
    90001 gagttcggga aattgactca acagcgcatc ttcatcgatg aggggaagga acccccgggt
    90061 ctccagtgaa cgcacggcgg ctgaggcggt ttcctgcaaa gactcatcga cctggtcgcg
    90121 gagcccgcgg gccgtgatgg tatagagcac cacggagaac gtcagcagga tcagggccag
    90181 cgccgttccg taccagaggg tgagccggac tcgaaggggc atcagtccac cttgagcata
    90241 tacccactcc ctcgtatggt atggatcagc ttgcgcgccc gcccgcggtc gatcttattc
    90301 cgcaaataat ttacgtagac gtcgatgacg ttggtgaatg tgtcgaagtc ttgattccag
    90361 acatggtcgg aaatcatggg gcgggtcagc acccgtcctg cgtgccgcat gaagtattcc
    90421 agaagggcat attctttaac cgtgagatcg atacgttgtc ctccgcgggt gacttcccgc
    90481 gtcgccggat tgaggatgag atcatcgatc tggagtgtgc cggacgattc cgcggtgccg
    90541 cgccgcagga gtgccctgac acgggcgagc aattcatcga tggcgaaggg tttggtgagg
    90601 tagtcatccg ccccggcatc cagtcctttg acgcgctggt cgactttcga ctgtgcggtc
    90661 aggatcaaca cgggcgtctg aattttttct ttgcgaaggc gggtcagcac ttccataccg
    90721 gggaggccag gcagcatcag gtcgatcatg atgaggtcgt agctgccggt cagggccaga
    90781 tcgaggccct gcgcgccgtc ctcacagagg tcgacggcgt aactttcttc ctcaagggct
    90841 cgcttgatga acgagcctac cttggtttca tcttcaacga caagcacgcg cataggtcat
    90901 gtccacaggt gggtggatta tagcagaggc ggacggccga tgatgcgatc aaacgtgcca
    90961 gtcagggaag gtcgggggag tctgaactgg agcgcgtggc ggtttgtagg cgacagaggg
    91021 tacgaatgtc cttccaatct tctccgggaa gcaactggcg cgcgtcggtc gggggcgggc
    91081 cggcaagcgc gagaagcgtg gcgagccgcc ggctcatatc gggatgggtc gagagaaaat
    91141 ccggcggttc gatatggtct tgctccgact tctgcagcgt cccgtaaaag gcaatcatgg
    91201 cggtcggatc gagatgggcg gcctgcatca tacgaaggcc ttcgacatcc gcttcttcct
    91261 catgggtgcg gctgtaatgc aatgatccca tcgtctgcgc accttgcagc ccccaggcca
    91321 gtccccctga cagatctccc gatacggcgg tcagcagcag cgtgccggct gtctgttcaa
    91381 ggatggctct ggtgctgtga cggagataga catgctggag ttcgtgcgcc aggactccgg
    91441 ccagctgctc ggcgctcccg gtctgctcca gcaggccgcg cagaaccacc acgtggccgc
    91501 cgggagcagc aaaggcattg atggcgggat tgtctaccac ggagagcgtg atccgatacg
    91561 gagagtccgg atgtgtggcg gccaacattt gcacgacctg atcgatcttc cgaagtcgct
    91621 caggctctcg acattgctgc gttgccggcg cgaggtgttc gacaatctgc cggccaagtg
    91681 attcttccca catgatcggt acgtagggcg tggctgctga ggcgatacca ggaatgcccc
    91741 atcggtacag gccgatgagc atgactatca ccccgagtcc cgcgcccagc gtccaggcaa
    91801 ggcgtctctg tcgccaggag ggatcgtgaa aatgttgtgc gagtgctggc gctgccgtgt
    91861 ggatatcggt caagagcgcc cggctggcga ccaccaccgc ctctgccggt tcagtcccga
    91921 actccaagcg gaccggctcg ccccggtacg tcccttgtgt ttggcgaatc tggccgtacg
    91981 gccattcctt cgtgctgccg tcgggcatgg cgatctgcag cgcggccggg cttatggtga
    92041 gggtgacacg gtgtcgtgtg gccgtgcggc cgtcgaggta gtgggcggtt cgggcggtgg
    92101 acatgatcac gcatccatgt cgaagccggt gtcgaggagg tttgataacc cttcgccggt
    92161 gaccgaggct tcggaggtgt cttgcaatac gcgatcgaga tcgacggggc cttggagcga
    92221 cagcgtaccc ataaaaaatc gggcattccg caccgtgacc cagggccagg cccatcccaa
    92281 ggtggccagg aggaggccga ggttgcccag atacagcgtg aacagccgtt gccaggtaat
    92341 ctcggatgag aactgggcgt ctccgaatga ggtgtgattc cagaagtatc gctgcttctg
    92401 tcccagcagc cagatccaca tcggcccgag cacacagggg atcaacaaca gcgtcagccc
    92461 cgcgttcgtc aattgcgcgg cgagggcgaa cccgcacagg gccaggacgg cataggtggt
    92521 aaatagggtg atggcgaagg ggaccatcac tcctgatcca tggccggtga accggaaacg
    92581 ctggttaccg aaataggtgt gggagtgcag aaacgcctga cgctgcgttt ggaaataggg
    92641 atagtaggtg cccagggtga ggatcgtaaa gaaccatccc cgaaaataca gtttgagaaa
    92701 atcgctggtc cgtcctcgaa acgagaaccg gatgccgcgc catgaggtgc gggtgcatcg
    92761 atagcggcgg gcattcacga tcgcgacggg gacatagagg aagaacacca ggccggccat
    92821 cacctgtagc aagacatcga tccactgcgg aagctccagg aaggtatgcg cggcgcttag
    92881 agaaaaatag ggaataccga aaaccagcat ggccttcagg aacccctgat agagttcctt
    92941 gccggtgccg tgatacgcga aacgatcccc tgcaaaggct gtgtggctga acaggtaccg
    93001 acggatcttc gccttggccc agaaatgata gccgcccagc gtcaccagcg tgaggcagac
    93061 gttcaccaca tgcatgccca ggagggtccc gcccattccg tgaaaggacg cgcgcatagg
    93121 ggcaggcatg gatacagccg cgtcggttgg taacgcggag tcatgaaccg cggaaggcct
    93181 gtcttgccgg gtcggcagga cttcggcaga ggctgacgat accgggtcga ccaccgcgaa
    93241 ccgggcgccg cacgtggggc aggtgctgcg agaggccccc gtcaagtgtt ccagggcgcg
    93301 gactcggtaa tggctgaggc agcggtgaca ggttatgcgt gcggtgctcg agtcgtcatg
    93361 cagtgccgca atgctcatga ttcaacaggc ggcccgatgg cccaaggcgc aggcctgttg
    93421 caatccttgc aggaatcccg cgctatcgcc ttttcctcgt ttcacgatgg atcgttcgaa
    93481 aatggcacgt ccattttggg gctggtgtga gaggagctga tcccacagcc tgagggcttg
    93541 atcccattgc ccgcgtgaag tatgcacgga cgcggctgcg ctgtaggcat cgaggtaggt
    93601 aggattcaga cgaatggcgg cgtgaagggc gatgagcgct tcgtcaggcg cgtttttttg
    93661 gagcagggcg gttccgcgtc cgtaataggc ttcatgatca tccggatcca ggttcaatgc
    93721 ttcagcatag gcgaagagcg cgtggtcgat ctgtccgtgt ttttggaatg cgattccttc
    93781 cgcgcgtttg gccttggcgt cagaaaactc tctaccgccg tatgcgcgca gcagcgcgat
    93841 cagttcgtga tcttcgtcgt gttgtgcgat ttccaagggg gtctcgccgt gactcttctg
    93901 gttcaccgga atgccccctt cgaggagcgt ctgcgcggcg gcgagatgcc catgtcggac
    93961 cgcccgatgg agcgccgaat caccacaggt gcaggtggct cggatgtcgg ctccattggt
    94021 aaggagcacc tcaatggtgt cggcatggcc ctgctctgcc gcagccaaca gcggagtaaa
    94081 gccctgtttg cccttcgcgc tgggattcgc tccatgttgc agcaggagcg tgaccacatc
    94141 ggtctgcccg cgcttggcgg cttcaaacaa tgccgtccgc ccattgccgt tgcgttggtc
    94201 caccgtttgt ccttctgtga ggagcgttgc gaccgcagac tgatcgcctc gcgccgccgc
    94261 aaggtggagg ggcgtccagc cattggcttt caaaataagt ggcgacaaca tcaacgcatc
    94321 gatgtcgtag ggatcgtggg tcgtttttac ttgccccagg atccagtagg ccatgcccat
    94381 caacaacacg tagttcaacg cacaggccag gacagccact gcgatgcctg tgagccggtg
    94441 tgaaagatcg attggaagat cgccgtccga cgatcgagtc ttcttgtctt catcggtcgg
    94501 ccgtgccgcc gggttgagga ccagaaggtt ggcatggcag gaagggcagg gaaactggtg
    94561 caaacgatga ccggcgactg gtgcgcagga ggtgattgcc tgacagaagg gacagatggc
    94621 agtctgtgcc gcatcggacg cggtcagcgt aggggatgca gggagtgccg tgccagggtg
    94681 aacggtagtc atggtgcctt acgacccttc gcccgggcgc gtgatggacg gtcccgtcag
    94741 tggagtgatc ggtgattact gaaaaggata gcgcaaaacg ggaggagcgc aaccgccggg
    94801 atggctgggg gcaggctgtt gtccgatgct gaacggaatg caggggtcgg gtcccaggat
    94861 tgacgactta ggctggcggg aaacgcgtaa tggtcacagg cgcataggcc agatggggac
    94921 cctcgggtcg gcggtagctt aatgagtggc gaagccaggc cgtgtcgttg cgggttggga
    94981 aatcgcctcg atagtgagcc ccgcggcttt cttcccgtcc taaagcgccc acaacgatgg
    95041 tctcagcgat ctccagcaga cactgcaatt ccagggcgtg aatcaggtcg gtgttgaaga
    95101 cgcgtccctt gtcctgtacg cacatgtctt ccgcgcgacg tttcaacgtt tgaatggcac
    95161 tgagagcctc ttgaatggat tgtttcgtgc ggaagatccc cagattcagg ctcatggttt
    95221 tgccgaggtc gtcccgtatc tgccaggccc gctcaggtcc ggggttgctg agcagcttat
    95281 cgatgcgctg ttgctcgctc ttcaggatgg tttcggagac cggtgacagc tgaacggtgc
    95341 ggatggactc agcggccttt gtgcctgtcc ggcgtccaaa gacaatggtt tccagtaagg
    95401 aattgcctcc gaggcggttg gcaccatgca cactgacgca ggcgcattcg cccgcggcga
    95461 agagtcctgg cagctcggtt tctccccacg cattggtccg gacgccgccc atctggtagt
    95521 gggccccggg acgtatggga atgggtgttt cgatcggatc gagacctgcg aattccatcg
    95581 ccagttcccg gatctgcggg agccgctcca gaattttggc gcggcccaga tgtcgcaggt
    95641 cgagcaggac gcagccgtcg acacctcgcc cttcctggat ttcctgtccg atggcgaggg
    95701 acacggtgga gcgggtggcc aactccattt gttccggcgc gtaccgtttc atgaaccgct
    95761 cgcccaacgt gttcaggaga tatccgcctt ccccgcgcgc gccttcggtg atcaggatgc
    95821 cggtgtcttt taaggtagtc ggatgaaact ggacgaattc catgtcctcg agcgcaattc
    95881 ctgcgcgata cgccagcgcc atgccatctc cggtattgat gacggcgttc gtactcgtgg
    95941 aaaaaatgcg gccactcccg cccgtagcca ggatcacggc tttcgcgcgg agcatctgta
    96001 acccaccatg gatcatgtcc caggcaacga cgccgcaaca gcggccctct tcgaccagca
    96061 aggccgtgac ataccattcc tcatacactt tgcagcggcg cttcatcagc tgttcgtaca
    96121 tggcgtgtaa gagggcatgg cccgtgcggt ccgctgcgta acaggtccgg ggatagccgg
    96181 ctcctccgaa cggccgctgc gcaatacgcc cttccgcggt gcggctgaag atgacgccca
    96241 tgcgctccaa ttccagaatg tcctgcggcg cttctttgca catggcctca atggcgtcct
    96301 ggtcgcccag atacagccct cccttggcgg tgtcataggc atgggcctcc caggagtcct
    96361 ctgcgccgat ggccgcgttg attcccccct gggcggcgac ggagtggctg cggatcggat
    96421 gcaccttgga gagcagcgcg acattgaggt ccggcggcac ggcaatagcg gcccgcatgc
    96481 cggcgagtcc tgctccaacc acgagaatgt catgggtcat gatgaagtct tcttgtcttg
    96541 gtcgcgtctg atgacggtca acgttgcctg tggcgcaagg tcgatctcac tattacgcta
    96601 tggaaattgg gaaaacaagc gaggtgagat ggccgctgaa cgcttggaaa actcggtgct
    96661 gcatgatagg gtacccactc tgcctcgaat gaacgagtgc gtcaccttat agcaggactt
    96721 cacccccatg cctgaaccgc agtttctcga gccgaacgat acgtttgtgc cccgccatat
    96781 cggtccgact gagtctgaaa tccagacgat gttggaaaca gtaaacgtgc cctcgctgga
    96841 ctctctcgtt gaggccacgg ttccttccga tatccggttg cagggctccc tggcggttcc
    96901 atccccgcgc ggcgagcagg aggtcttggc ggaattacgc ggaatggccg ggcagaatca
    96961 ggtctggcgg tccttgatcg ggatgggcta ctacgattgc atcacgccgc cggtcattca
    97021 gcgcaatatc ctcgaaggcc cggggtggta cacgcaatac acgccctatc aggccgagat
    97081 cgcccaaggt cgcctcgagg cgctggtcaa tttccagacc atggtggcgg atttgaccgg
    97141 cttgcccctt gcaaacgcct ctttgctgga tgaggcgact gcggcagccg aagccatgac
    97201 gatgtgcgcc gcgatttccc gcgcggccgg tcacgaccgg cacaaatttt ttgtgtctga
    97261 gacctgtcat ccgcaaacga ttgcggtggt gcagacccgc gcggaaccct tgggcatcgt
    97321 cctgcaggtc ggtgcgattc aatcgttgga tttgtcctca ggcgaattct tcgggatgtt
    97381 gctccaatat ccctcgacgg acgggtacgt gggcgattac agcgaattca ttacgcgcgc
    97441 ccatgccgcc ggagtctatg tggtcgtcgc gactgatctg ctggccttga cgctgctgcg
    97501 tcctccggga gagttcggcg cggatgtcgc ggtggggtcc agtcagcggt tcggtgtgcc
    97561 cttgggattc ggtgggccgc atgcggcctt catggccacc aaagaagagt ttcgacggca
    97621 gatgccgggt cggatcatcg gtgtgtcgaa agatgtcacc gggcgcccgg cctaccggtt
    97681 atctttgcaa accagagaac agcatatccg tcgggagaag gcgacgagca atatctgcac
    97741 ggcgcaagtg ttattggcag ttatggctgg gatgtatgcg gtctatcacg ggcctgccgg
    97801 actacggcgc attgcggaac ggattcacgg tctcacgatg gccttggccg aagggctgcg
    97861 ccggcatggt tgcgcggtcg gactggagcc ggtcttcgat accctccggg tcccgctctc
    97921 gccggcacaa tcggagacga ttgtgaaccg ggcgcggcag cagaagatta atctccgccg
    97981 atacgatgat cacagcctgg gactgtcgct ggacgaatgg agcacggtgg atgaagtgcg
    98041 gcagttgctg acgctcttcg ccggccagga gatttccccg gaagaactgc agaccattct
    98101 tgaatcggtc gacgggcggt atccggccgg gcttgctcgc accagccagt atcttacgca
    98161 tccggtgttt caccggtatc acgcggaaca tgagctgctg cggtatatcc atcgtctgca
    98221 atcccgggat ctctccctgg tgcattcgat gattcccctt ggttcctgca cgatgaagct
    98281 gaatgccacg gcggaaatgt tacccgtcac gtggccggag ttcgggcggc tccacccgtt
    98341 tgctcccgcc gatcaggtcc agggatacct ggccttgttc caacagcttg agtcgtggtt
    98401 ggcggagatc accggattcg ccgggatttc gttgcagccg aatgccgggt cacagggcga
    98461 atacgccgga ttgatggtga tccgggccta tcaccggcag cgaggggaga cgcagcgcga
    98521 tatctgtttg attccggttt ccgcgcatgg caccaatccg gccagcgcat cgatgtgcgg
    98581 catgaccgtc gttcccgttg cctgtgatca gcgcggcaat gttgatctcg cggatctgga
    98641 ggccaaggcc acgcagcatc gtagccgctt ggcggcgctg atggtgacct atccatcgac
    98701 tcacggcgtg ttcgaggaag gcattcgccg tatgtgccag atcgtgcata cccatggcgg
    98761 acaggtgtac atggacggcg cgaacatgaa tgcgcaagtc ggtctctgcc ggccggccga
    98821 tctgggtgct gatgtctgtc atctgaactt gcataagacg ttttgtattc ctcacggcgg
    98881 cggtggtcct ggaatgggac ccattggtgt cgcccgccat ttaatgccgt tcatgcccgg
    98941 acatccggtc acgaaattgg gcggaccgca gtcaatcggg ccggtctcag ccgctcctta
    99001 cggcagtccc agcatcctga cgatttcatg ggtctatatt gcgttgatgg gacgcgaggg
    99061 gctgaccaaa gcgacgcagg tggccatcct caatgccaac tacatggcca aacgcctgga
    99121 gaagttctat ccggtcttgt acagcggggc acgcggattc gtggcgcatg aatttattct
    99181 ggatttacgt cccctcaagg acagcagcgg cgtggaagcc atggatgtgg ccaagcgcct
    99241 catggattac ggattccatg cacccaccgt gtcattcccc gtggctggca ccttgatgat
    99301 tgagccgacc gagagtgaag tgaaaagtga gctggatcgg ttgtgcgacg cgttgatcgc
    99361 gatccgtggg gagattcagg ccattgcgga aggtcggcag ccgcgctcgg ggaatgtgct
    99421 gaagaatgct cctcacacgg caatggccgt gactgcctcg gaatgggcaa ggccctactc
    99481 ccgcgaagaa gcggcatttc ctgcttcatg ggtgcgcgag aacaagtttt ggccgagtgt
    99541 gggccggatc gatgaagcct atggagaccg ccacctcttt tgcacctgtc cgccggtgga
    99601 tgccgtctcg tgattctgct tctggcggcg gcacgtggtt gaccgctgtg ggtcgtgtgc
    99661 ggcgtgtcgt ccgtcagatc gcgataggtt gtctgctgct caatatgttc ttcgccctgc
    99721 ccgtccacgc caccgttgaa tccgttgctg ttgcggacac tcatatcgag gatagtccgc
    99781 tcgacgtggt atccggactc acctatcgtg cgggcacacg attgagaatt ccggggactg
    99841 gctggtcatt tgtggttccc gatggttggc agagcaatcg gcctgacgat gcagagatgc
    99901 cgtttctcat tccagaggaa ggcaaggaac tgggcatgat gtttccgctg gccaaggtca
    99961 cacgagacgc ccttcgtgaa caacttcgtg agccgctgtt gctgttgcat ggactctcct
   100021 ttatcccagc gggcgcgcaa gcagaaaccg acgcatggat cgcgcaatcg tacgagggag
   100081 aaggaatggc cggccgggcg ttggcggtca tgggagcgga caatacggcg gtgatttatt
   100141 ttctcatggg gccgccccac aacatgtccg tcctccagtc cgtactggaa cagcttggcc
   100201 aatcgacacg cttcgtccag tccactccga ggcgtgacgc cggcttgtaa gacgcagtga
   100261 tccgtgcatg tgtcgggacg aaggtggatc cctccctctt ctctcccgac gtcgtatcat
   100321 tcaggcatgc agaagacttc tccaaccaaa gtcattattg ataccgaccc cggcgtcgat
   100381 gacgccctgg ccattctcct ggccttggcc tcgcctgagt tggacgtggt gggaatcact
   100441 actgtctgcg gcaatgtgcc agtgggacag ggcacgaaga atctgtttcg cctcttgaat
   100501 cttctcaacc ctccgcctgg cttgctggtg ggacagggcg ccgcgcggcc cttggaagaa
   100561 gcccttgaaa cggccgttca cgtacatggg agtgacgggc tgggtgaact ggatgctctg
   100621 ctgtctgcag agggaacgat gcggtacccc gctgttcgtc taccgcctct cctgtccact
   100681 gcccaagatg tgtggaacga atgtgtgcgg cggtatcagg acgacatcac gcttattacc
   100741 ctggggccgc tgacgaatgt ggctgtggcg ctgaaagtca atccacttac cgtccagaag
   100801 tttcgttccg tgattgtgat ggggggtgcg atcggtgtgc ccggcaacgt atcaccggtg
   100861 gctgagttca atatgtatgt cgatccccac gcggcccatc gggtgtttca ggcggcgctt
   100921 ccattaaccc tggtgccgct ggacgtgacc acccgtgtcg ggctgcgtcg tgatgtcctg
   100981 aagatgtgga ccggtgcctc gcgcgatcca cgctgtcgta tcgtggcaga catgaccgcc
   101041 aaggcattcg attttgcaga acaggttgaa gggcnnnnnn nnnnnnnnnn nnnnnnnnnn
   101101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
   101161 nnnnnnnnnn nnnnatcgtg gcagacatga ccgccaaggc attcgatttt gcagaacagg
   101221 ttgaagggca tggattgctg tacttccatg accccatggc ggtccttgcg gccattgaac
   101281 catcattgct caaaaccgag ccgctgcatg tcgaagtgga agtggtgggg cgtgtgtccc
   101341 gcggagccac tgttgcggac cggcgccatc gcaggccgga agaaaaagcg aacccgaaca
   101401 tgcaggtcgc ggtggacgtc gatgccgagc gcgcattgag tgtgatccgc agtcgattgt
   101461 gtccatggtc gtagttgtcg gttccagcaa tatcgacttc accgcggtag tcgatcggtt
   101521 accggctcgc ggggaaaccc tcctcgggca tcgattcctg caatcgttcg gcggcaaggg
   101581 ggcgaatcag gctgtcgcgg cagcgcgagc cggagccgac gtcgcgttcc tcagcaaagt
   101641 gggaaatgat ttcaacggcg acctgatagc acagcatctg gctacgcagg gcttgtcacg
   101701 actcatgctg ttgcgtgatg ccgcagtgca gaccggggtc gcgatgattc ttgttgacgt
   101761 cgcgggcgac aatcagattg tggtggtgcc aggaagcaat cagcagctca ccccggcgga
   101821 tgtcgagcaa cacgcccagc tgatcgccga cgcgcgagtc ctgctcgttc aaatggaaat
   101881 tcccatcgac acggttcagc aatgtctacg gctggctaag cggtatggac tccgtacgat
   101941 tctcaatcca gcgccggcct gtccgttgcc accggagtct ttgcggatgg tggatattct
   102001 cacgcccaat gaacgtgagg cctgcgtact ctccggtgcc acagacccgg ccgacgcggc
   102061 cagagtgttg gtcagcaccg gggcggaaac agtcgtcgtc actcgcggta cggagggagc
   102121 cgtcgtctca cgcgggaagc acgtgacgca tattccggcc tttatcgtcg acgcgattga
   102181 ttcgaccggt gccggggata cctttaatgg ggcgttagcc tgtggcttgg ctgaaggcct
   102241 gtcgattgag gaagccgtgg aaatggccgc tgcggcgggg gccctggcta cgacgggata
   102301 tggcgcgcag gcagcaatgc cggccagggg cgatatcgac cggcttcgcc gaaccggagt
   102361 caggcgaatg ccgccgcgtg gttaattgcc tttggtggat tgggcccgca gttgtgcaag
   102421 gacttggcga gcttccggta tgaaatcggc gcgctgcagt tcttgggcaa gatccagggc
   102481 cttcgtcgca atggcgacgg cttcttcatg tcgcccgtcg gcgcagtagc ttttggccag
   102541 actcaatcga gcattgacgg ctgctggttc acgagcaatc acttgacgca gcagtagttc
   102601 tcctttttcc ttatctcctc ccatgaatcc cggcacgcgg accaatacgg tgcctttggc
   102661 tgacagtgca tccaaatgac tcgggtccag ttcgagggtc cggttcaact ccttcatcat
   102721 gcggcggaat ccaaacagcg acgagaggct ttctccgtcc acccgaagtt gctcgccgag
   102781 attacagaac agggcaaaat gcgcgtcagg tgatgattca tccgccgcaa cggcttgctc
   102841 gccgaatgct tgccctgacg caaaatgttg aatgcggtcc gcacgagtct gcgcctgccg
   102901 tcccagggaa cattcatgca tggactcggc agtgaggcgt tgcacggcgg taccggcaac
   102961 agcttgctcg ccgatgagag ctacgccgag caagccccac aatgcgataa cgggatagtt
   103021 catgaagcac cgatcatgtg agatggagtg aaaaagagga cgaatggtac agcaacgacc
   103081 tcgctgaatg ccagagtata gcatgtggtg ggtagcgtca tgggagaact tatcctagag
   103141 aagtcctgac ccacggtgta agcgtgcaaa acgcgtccaa gagagcgtga taagggtatg
   103201 ccgcgttgcg tccactcgga ttgggcagca acacgatact ggaatcgtac agcgtcaccg
   103261 attgcagtcc gggtttgcgc cctgcttgtt tgggttccaa gggaaagagg atgggataga
   103321 gcgtgatgcc gacaagcgcg gtcacacgcg ggcgatatcg acgaagtttt tccatcaggg
   103381 ctcgccggcc tgtcgcatag tcttcggcgg tgagatgttc cgatccggca gtcgggcgcg
   103441 cgacaaggtt ggtgagtccc aggccccatt ccagaagccg ttcgtcgtca tggtaggtga
   103501 ggggttccgg caccagggcc gcatcataga gcagtttcca aaagcggttt gaatggcccg
   103561 catagtgatg gccgactgcg gccgagcgaa gcccgggatt gatgcctacg aataggatgt
   103621 tgagattcgg tgcgagatgg tccggcaagg aaagtggcaa cggtatcccc gtgcatgatg
   103681 tgtctggaag catctattgg ttgaatgcgc ctggcctggt gtatcggatt tgagagtcgc
   103741 ttaatgaggc ggtttagtat cggcaaggct attctgccac agaatggacc ccggcgttat
   103801 gaggtatttg aaccatttca acagtcgatg tgcagaaaaa caataagtta cgcccgccat
   103861 ccatacggca aggaccttgc tataacgaac acccgtcggc ggtcggttac ttcacaccta
   103921 acaggaggta gtcagtatga agaagatgat gttgacggtc atggcggcag cgttgttggt
   103981 ggcgttcagc gcgccttcgt tcgctggcga cgacaagaag aaggaagaga agaagggcgg
   104041 ccacttctcc cagaccctct tcggtgacga caagaagaag gaagagaaga agggtggaca
   104101 cttgtcccag accgtgttcg gcgaagagaa gaagaaagac gagaagaagg gcggccacta
   104161 agagtcaccc gttttttggc gaggctagct gtctgaagag gtctcccatc ctgatggtgg
   104221 gaggcctctt cggtcgttta ccggttcccc acctgccgaa tatagaccgc ttgtaatccg
   104281 acaatcgttt ttgcgtcccg tatcgtcccg tcttctattc tgtcgatagc ctctttcagg
   104341 ggcatctcta ccacgtccag aacttcgtcc tggtccagat tctgggtgcc caaggtcagc
   104401 tctgttgcga gatagacgtg gatgacttca tcggcaaagc ccggagctgt aaaaatgctg
   104461 gacaacagct ggaagcgacc cgccttgtac ccgatctcct cctcaagttc gcgagcggca
   104521 cagtctaagg ggtcttcgtt cgggtgcagc ttgcccgccg ggatttcgta gataaacccg
   104581 ttggccgcat ggcggaattg gcggatcagt accaccgttc catctttctt cagcgggacg
   104641 acagccgacg cgccgggatg gcggatgact tcgagatcga tcgtgtgccc gttcggcaag
   104701 cgcaccgtat ccacgttgag tgtgacgacg gtgcctttgt agatcggtct gaccatgacg
   104761 tgcgcggact atggcgtgct gggctgcttc ttgtagaacg gtggtttgac gacgacggca
   104821 gggcaggatt ttcctctgat atcgatcagg atggacgacc ccggttggga cgcagccgga
   104881 gtgacatatc ccatgccgat gcctttttgt aggaggggcg acagatttcc gctggtgact
   104941 tcgccgatca cggcatgttg atcctgcgtg ctcaaaatct tgaacccatg ccgtggcacc
   105001 gctttttcga cgagctcaaa ggcgacgagg cgccgcgatg gcccgtcgtt gtgctgtgcc
   105061 agcaagggcg tccgcccgat aaattctccc ttatcgaact tcacgaccca gtccgccccg
   105121 gcctcaatgg gtgaagtgcc ctcatccatg tcattgccgt acagcaggta ggccatttcg
   105181 agtcgcaata aatcgcgcgc gccaagaccc gccggcttca tgccaagcgg gtgccccgct
   105241 tcaaggagac gattccagat cttcatcgcg ccttcggccg gaaggtacaa ttcatagccc
   105301 aattcgccgg tatagccggt tcgtgtcacc agggttgcat ggccacagac gacggcgtcc
   105361 aggcagtgcc ggattttcag gagcggaaga tcagtcaggc cgacggcggt gagaatgtcg
   105421 cgggaagcgg ggccctgaat cgcaatttgg gcaagggtgg ttgactggtc ctggacggtg
   105481 catccctggg cttgcgcaac cttttcgtga aaccaggcaa cgatcttttc acgattggac
   105541 gcattcacgc agaccagaaa ttcatacggt ttgacgtgat agatgaagat gtcgtcctta
   105601 atcccgccgt tcggcgcaca gaccatcgag tattgcgact ggcggaccga cagtttggag
   105661 acatcgttcg tcgtgacgcg ttgcaaaaag gccaatgagc cgggccctgc gacacggata
   105721 cgccccatgt gactgacatc gaacagtccc gcatggcggc ggaccgtctg gtactcatcg
   105781 accacgccgg aatattgaat gggcatctcc catccggcaa aatcgaccag cttgccttgt
   105841 gcgttgcgat gcgcgtctat gagtggcgtc tgtttcatcg gatgtgcgtg aaatccaggg
   105901 tgaagatacg cagaggccgg accatcatcg ggccttatcg gacttcgagc aattctacgt
   105961 caaacactag cgtggcattg ggaggaatca ctccgccggc gccgcgcgct ccatacccga
   106021 gttccggtgg aattgtcagc ttccgcttgc cgccgacctt catgccggcc acgccttcat
   106081 cccatccgcg aatgacctgc cctgctccga tcgggaagga aaacggatcg cggcggtcga
   106141 cggagctgtc gaacttcttg ccgtttgtca gccagccggt atagtgcacc gtggccagat
   106201 tgcctgcggc ggcttcgcgc ccggtgccca caacgacgtc tacgtatttg agcccggatg
   106261 cggtcgtgac ttctgccgtc gtcccgggtt gttcactgtt cgccatcatt ggcctcccta
   106321 tggtcatgaa tacgagcccc acgataccgc aagttgtcca ccagaagcga gtcgtcatac
   106381 gtacctttca gttcctgtgt gacctgaatc acaaatagga cactcggagc actcagcggt
   106441 tgagccctgt ctgatgccct cgctcaggtt ccgagaaaat ggcgatgctg gccgtatagc
   106501 catggagaaa gttccgcccg cccacggggc ccacttctcc ctgcgcaaaa aatccggcga
   106561 gcggaatagc gcctaattgc tcgcccaaaa ccgatgcatc gtgatgcgga acgccaaaga
   106621 gtcccttgcc gcgtccgcaa caactgaaca gcagcgcgcc gagcggtttg gacgggacgc
   106681 cctgaggagg tgcggcgagc aaggcatgaa gatcttcatc cgcggaatgg gcatcccgga
   106741 cttgaaactg gacggtctgt ccttcctgga tgacatcacc gatgacgatc gcgccggtct
   106801 gctgatcggc accgagtaga ttgcggatca ggaaatcgcc gagagtgaat tgcgcgcgtt
   106861 gctcatccat tgcaatgccg atatgcaatg cccgttgtgc ctgggcccgt tcatcgctgc
   106921 tgagctgttc aaatacggtc tgcaagcaat gcaaagccgg catgccgccg agctcttgaa
   106981 tcacgttgtg ctcggacttc gtgacgatga agcggtcacc gatgggacgg cagccttgcg
   107041 aaatgaccga gcggaccgca atattgccgg acagggccac gccaaccaat ccgtcggtca
   107101 acacctgatc gtcgaggaat agacgatttt cgccgaggtc ctgcccgccg ccggcgagcc
   107161 cgcccagggt gcgggtaccg gggtactgtt cctcaatgac ggaaaggact tcttgcatgg
   107221 gcgtcgaaaa gggatcggca aatagcatca tgacaggggc cgtgtccccc acctcatcca
   107281 gatccggcca attgcgcagc gaaaactgat catggacgct tgaaaaggaa agtcgtagcg
   107341 gttggacgag cacgccgggc aggtgagcgg cccagagagt ggccgccggt cctgattcga
   107401 tctcacgccc ggtcgcgatg atgccctcgc ccgtacagcc aaccagcgtg actgggccca
   107461 aggcggtgcg aagagcttcc gaaagtgcct cagcctggtc cgcatgctga gccgaaatga
   107521 acaggaaggc cacatcgatg cgcgacggcc ccagttgctc gcggatggcg tgaatcaagt
   107581 cagcggccgc agcctgagcg tccgcgtggc gtgtcagagc ggaggcaaat cgtagcgttg
   107641 acggggcagt cataatcacg ggatgtcctg ctgaataggg atggacggcg tgccgccgag
   107701 ggggtcagac gccaggattc atgcgttcag gtcgttcggt ggcgagcttt ttgtagtaac
   107761 gccacatgta cgaatgataa tcatccagct gcgcgagcaa ataatggagc tcggtcgggt
   107821 ctcgcgtttc cagggcttgc gccattcgcg tctttaagaa gtccgcaaac ttatcgctct
   107881 tttcgagcgc ggtgagaagc gtgagtccgc ctcccatgga ataatcagcc atagccctcc
   107941 gcatccgata cagacgagcc aggagaatag ccgtgagtac ctgtaaaagc aaggtgccgc
   108001 ccggcctcgt tcgtctcgtt caccgcccga tggctggatc cggtgacagc agtgtgcgga
   108061 gtcgcggaag cgagattgcc tcggcccgga atccatcccg tcccgtcacg gtggtggcgc
   108121 ccgtcagcgc gttgaggacc gcttcttccg tggcctcaac cgtggcggta atcacggcat
   108181 tcaaatgcgt gtcagccaga tgcgtgagtg aaaaagttgg ctcggtcggg taatggggga
   108241 tgacattgcc ggtggagaag gccagcatga aatcgccgct tccatggcga gcggtcgagc
   108301 cggttcgagc cagtcccagt gcggctcgct ttccgagtct tgtcagttgc cggctgtcga
   108361 gcggggcatc ggtggcgacg atgataatga tcgatccttc ggcgttaccg ctggatcctt
   108421 cacggctggc atgcaggcgt tccggattgg attccagcag tgcaccggat tgtgtggggg
   108481 cggcatcata ccgttgtccg accggcaccc cacccatcat gagttcaggg cgacgaccgt
   108541 gattggcgtt aaccaacacg ccgaccgtaa acccgccggc ggcagcagga aggcgccggg
   108601 aagctgtgcc gatgccacct ttgaagccat acgaaatcat gccggtaccg gctccgacgg
   108661 ttccttcgga gaccggcccg gatgccgcat cgtctaaggc ttgcatgaca tccgcttcag
   108721 agacatggcg gccctggatg tcgttcaacc gaccgtcatc acattcggca acgaccgggg
   108781 tgagggtgtc gtcggtgatg ccgattcccg ggtagcggcg gagcatccag ctgatgacgc
   108841 cattggcaac ccgcggcaca ttgagcgtat tggtcagggc gatgggatat tcgagaaatc
   108901 ccgattcggc cacccacgcc agaccggtca tttctccggt gccattcagg acgaaggccc
   108961 cggctgggac tttcttgtgc cagacatcct ctcgtggcac gatgaccgtg acgccggttc
   109021 tgactggccc ttgccctggt tgaagcggtc cttctccacg cctcaaggtc acctgcccga
   109081 ccttcactcc cggcacatca gtaatggcat tcagcgggcc gggctccaat tgtccgagtg
   109141 tcaggccgag ggcgcgagcg cgtacccgcg gttcttcagt cacgtcgctg agcgtctctg
   109201 cgctccatcc ggaatggacg cctgtcgtca tcacgagaac cacgcaggcg atgatcgaaa
   109261 aggacccggg gtgtgaatgg ctcatgttga cggcatcctc catacgtcat cggctgtgaa
   109321 gcgcggagtg acgctagact tcataactac tttctttgtg cggcacgaca atcgaagttc
   109381 gtgggtattc ggcctggatc gccgccgcca gtgacagtgc ctgtttttct tctccatgca
   109441 ccacgaagat gtgttgaggc ggaggagaaa tcgctctcac atacgcgagt agatcgttcc
   109501 gatccgcatg ggccgacaat ccgttgaaca cgacgacctg cgcgcgccgt gccgtgggaa
   109561 tgccgaacag cggtacgaca tcccagccct caaccagcct gcgtcccagg gtatgttctg
   109621 cctgaaagcc cacgatagcg atgatattgg cttcgtcctg aatggcatgt ttgaggtgat
   109681 gcaccacgcg tcctccctca cacatgccgg atgacgcaat gatgacgcag ggacctttca
   109741 cgctgttgag tcgcttactt tcatccggtg atgagacata ccggatatac cgggcggcga
   109801 agggatcgcc ttccgaagtg aacgtgcgat acgtctcttc gtcgtaacac tcagggtgct
   109861 tgcggaacac gtccgtgacc ttcgacgcca gcggtgaatc gatgtaaatg ggaagcggat
   109921 cgatctggcc ttcggccacc aaatgtttga tgcgcatcac gaggtcttgc gtccgtccga
   109981 gtgcgaatgc ggggacgatg atcttactgt tatgcgcttt cgcgtgtgcg acgagctgtt
   110041 gcgctttctt ggtcagggtt tccccggcct cctcgtgaag tcgatcgccg taggtggatt
   110101 cgatgatcag cacatcacaa ctcggcggcg gcgtcgggtc gcggaggatg ggcatgtggg
   110161 atcgtcccaa atcgccggag aacagcacgg tggtactgtt gccgcgcgcg gagtatttga
   110221 gccggatggc ggcggacccg agaatgtgtc cgacatcatg gaaggaagcg gtgaggcgag
   110281 gcgtcacctt gatggtgtca ccgtaacgcg cgccaacgaa acggcggatg atggctcgcg
   110341 cgtcccgtgt ctcatagaac ggcttcaaac acttctgtcc gcgacgatgt tctttccggt
   110401 tcaggtaggc gcagtcgttt tcttgcaggc gggccgagtc ttccagcatc acggcggtca
   110461 ggtcggccgt ggcacgagtc atgtggacat ccccgcgaaa ccggtgctgg cctaggacgg
   110521 gaagggcgcc ggagtggtcg atatgcgcat gcgacaggag cacggcttga atcgagcgcg
   110581 gatcgaatcc taagaagcgg ttttgccgat cagcctcctg ccgtgggccc tgaaaaagac
   110641 cgcagtccaa caggattcgg ctgcctggca tttccagaac atgacggctt ccggtgactg
   110701 aacgcgcggc gccatggaac gagatcttca tcgtgcggca gggcctccat gggtctgact
   110761 gatcaggtcc catgcctgtt gagccgtttc tgcaaaatgg aagagcgcga gatcgttggg
   110821 accgatgagc ccttcatctg ccagcatgga ccaattgatg agtcgctccc aataggcccg
   110881 cccgatgagg acgatcgtaa tgttgcgggt tttgccggtc tggagaaggg tcagggtctc
   110941 gaagagttcg tcgagcgtgc cgaacccgcc gggaaaaacg accagcgctc gggcacgaag
   111001 gagaaaatgc attttacgta acgcgaagta gcgaaattgg aaacacaaat ccggtgtgat
   111061 gtaggggttc ggttgttgtt catgcgggag ggtgatgttg agtcctatgg acttggcgcc
   111121 ggcgtctgcc gccccgcggt tggcggcttc catgatgccg ggtccgccgc cggtgacaat
   111181 gacgaagtcg cactgcccgt tgatttggca cgtcgcggac accaggcggc cgaattcacg
   111241 ggcgatgtca tagtagtgcg ccatggccag gcggcgttcg gccatcgtca cctgctgctg
   111301 tcgcagcgca tcctgcggcg cgatctgcaa ctcggccttc gcggcttcta gcgcctggcg
   111361 cgcaagggaa ggttcaacta gccgggaact gccgaagacc acgatcgtcg aacggatgcc
   111421 ttgttctatt tggatcaatt ccggcttcag gagttccagc ccgagacgga gcgggcgtaa
   111481 ctcatcgcgc tggagaaagt cgatgtcgcg atcggcgggg atataggaag aagacgcgct
   111541 gtgaggatgt cggctcgctg actcgtcgga ggaccctgcg cccatgagcg gcattataac
   111601 gagatgggag caaggcagca attcgtgaac ggcgatggtg tctgggcagg ttaaaacgga
   111661 tagggtgtaa acaatggatc cggacgatga cggacgaatg cctggtgtgt cacgacccag
   111721 ttagagtcgc acaggagctc gaatgcgtcc gtgcccatgc cggagcgaga gaagacgaga
   111781 tccgggtcca cgggggacca gggcttgacc agccagccaa agttcacccg cgagtacttg
   111841 gcgttgagaa accgataggt caccactgct ccaccctcgg catcggtaat ggagtcgggc
   111901 gcgcccaatt tgtttacgac atcggtcagc gtcgtctgcc cctcggtaat gaaagacact
   111961 gtttggggag tcagtggagt attgatggtc aggcgcgcga cgttgcagcc ggacaagggc
   112021 atcatcagta tcgcggcgaa aaggaatgtg cgcgagacag tcctgggtaa cacggcgtca
   112081 gtctccgaag ggccagaacc tgaactccag attttcagtg cgcttcgacg cgatcacatc
   112141 ttccacgatt ccatcgcgat tgatgatgac aaacaggtcg tcgcttttga ctgacacgcg
   112201 ggaaaagttg atcaagatga ggagcaagct gccgaccttg gcgtcgtagc ggtagtattg
   112261 aaagagatcg cgtccgttca gttgcagcag acggtctggc gcgccgagtt gggccaggac
   112321 atccgctcgg gtcgtgacgc cctttttgat cgagttgacg gtgtccgtct tgatctcttc
   112381 gcccagggtg cctcggctga atgcgcaggc gttcagcgtt agcaacatcg cgcccagcaa
   112441 caggcccgct cgtcgcatat gtgatcctcc ttatgcgtat cacccaccgg gccggctacc
   112501 ggcggtcggg ggaagaaggt ttgggaagga cgaaatcgga ttcataaatc cggtaggtcg
   112561 acggggtaag tccgacccgt tcatacactc cctgggcgac ggtgttgtcg ccctctacgt
   112621 acagacggac gccgcacaca tccgctcgcg attgcgccag ggccatcact gcttgatgca
   112681 tcgcacggta cacgccttgg cgtcgccatg cggggtcaac gtagacgctc tggatccacc
   112741 agaactgggc attccgccaa tcgctccatt catacgtgag gagcagttgc cctacggtca
   112801 ctgtatcggc cggagcgttt cggtgcaagt ccgcgacgta atattgtccc tgttcaggtc
   112861 ggtcgatgac ggcttgtgtg ccaagtctga gacgcgcgag gtcgagtcgt cggtgttcgg
   112921 tctcccaagc catggctgcg ctgaatgtcg taaggacgtc cacatcctgc acggtcgcca
   112981 gtcggatgcg taactgatca gcggagatca tgacgacttc accgccgggg ctgtgagcca
   113041 gccctggagg gggcgataca agcccgctga gaattccaac ttgagcgctt cttccggcaa
   113101 gaggttcagt ggctgcaatt gggttttggg aatcatggcg agatagccgg tgaacgggtg
   113161 aatggcagtc gggacgaata ccatgaccaa ttcgaccgat ggagcgactt gtagtgcggc
   113221 tgggggtggg cccatgacga aaccgagcgc ccagagccca tcgcgcggga acgggaacgc
   113281 gaccaccgta ctttgtccga agcgcgcgcg gtagttcaga agatccgtca tgctcttcaa
   113341 agtcaaatag atgctgcgga tcaatggaat gccttcgagc gtcgtttcgg cccaacggac
   113401 gaagcgttgc ccgatgacct gtgtagcaat ggctccaacc atcagcacca tgccgatgag
   113461 cagcagaatc cccaaccctg gcgcgtaggg ctggatctga tgaccgatca ggttgagcag
   113521 gaacgagtcg agcgtttcaa acagggccgc caggatcaga aaggtcgtcc aggccggaac
   113581 gagcaggagc agtccggtaa aaaagatgcg tccaagtcga tgagaagatg tcacgctgag
   113641 tcctggtgtt ggtgcgcgag aggggtaacg atcatctacg ataccagatg gggatgagtt
   113701 ttggtatcta cttgatctgc cgattttttt ggactatgtt tcgggtaacc tttgagtcac
   113761 cctgtcgggt gagattgtga gattcatcgt gagcgatgcc gtgcaaaaga aagtcaatct
   113821 cgacaaagac ttgttggcca ttctgtgttg ccctgaaacc aagcaggcag tcgtattggc
   113881 tgacgatctg ttgattcaaa aggtgaacgg agcgatcgag cggggagtat taaagaacaa
   113941 ggcgcaaaag gctgtcacgg aaaagctcga tggcgggtta gtgcgatccg ataataagat
   114001 tctctaccct gtgcgtgagg atatcccggt catgttgatc gatgagggaa ttccgctcga
   114061 ccaggtggcg tagttttccc acctcgaatc ttctcaggtt acctcacaag catgttgatg
   114121 ttggatgggt gccctcaggg ggcaatgcac cctgtctgta tcccttcgcg cccttgttcg
   114181 ggagttcctc tacatcgcga aagacgagga caggctggtg gagactacgg ggctacgaga
   114241 ccgctctcgc ctgaggcgct ctctgttttg gagcctgtag aggttccgca ttgcgcgcag
   114301 agatactcgt acagatttcc agtgggtaac acgagaagca ggcgctggcg ggtgggagtc
   114361 gcttgacggc aatgaccgca gtagagcaag gaggcgttaa atgaatcgta ttgatccgtt
   114421 tgacggtggc cttgaggaga ctggggggtc ctccggggct gtgtggcacg cggaggccaa
   114481 ccaggtggtg acggtctgtt cgaacgtggc tgcatggagg gtattctact gagagctgga
   114541 tagagcgcgc aatggcttat atgggatggt cgtcgagcaa gggtcagcga atatgctggt
   114601 cttctggaac atcgggctca tcgccgtttc gtccgcgaaa aacccaccac agcgaccgca
   114661 ggacctgcac cacgatatag agagtcaggc cggaaagtaa tccgtacagc caggctttac
   114721 tccacggcca catgtccggt gcatgcagaa cgacggcgag ggcaatatgg cctcccaagc
   114781 cgagacaaat ggcgaagatg atccactcac gggccatagt cgccctcgga ggaggccata
   114841 acgaaggata agcctggaga caatcgacag gcggttacac ggaggttttt gcggactcga
   114901 tctcagttgc cgtgatcagg ctggacaaat agtccgcaac gaaggtcagg gcctcctcgc
   114961 gtccgtctgc ccgtcgaccc ttttcccgac gttgcacgga gagctcatgg tccaagtcgg
   115021 atttcacttc cgtcagtacg cgttgcaacg tcgtcggcgt gatgttggcg tccatgtcct
   115081 cgagttcctg gcagcgatcc aggacccaat tgaggccttc gatccgcccg gcataatgtt
   115141 cctgctcggc ggtgtcgatg cgggacatgg tcgtcgcaaa ggtctgggct tcgtacacca
   115201 gattatagaa actcgtaact gcgcgttgta acgtgggatt catagtgctc cttggctcat
   115261 taagaggttc tcgtgattat acctgagatt aggggggcga caaggaattt ttttgatgaa
   115321 ccgcgactag gtgaacagga gggagtgaaa ctcgttcatg aacccgtagt acagcggggc
   115381 aaaatctttg aggctgtccg gactgttctc tttgagtcgc ttgaagaaaa atacctgggc
   115441 gcgaggatcc aattgctcga ggaggcggct cagctgggcc atgctgtaac tgccggccgg
   115501 aacgctgagt acgtagtgga ccagacgttc gacgaactgc tgttccacat actcgctgac
   115561 aagcaatccg tctggaatct tgcctgatgc gagatactcg tcgaatgaaa cggcttgcgc
   115621 tgcaaagggt gccgactgct gcgggcggga ggtcacgcta cggttacctc attgatagga
   115681 atagcgaaaa gcgacattta cattcactgt actcaagcct ctcaagtccg tcaagtggac
   115741 agaagaagca ggcatgtctg agtcgaaaga ccaagatggc ggtggcagat gggcactctg
   115801 tgcggcgaac ggtttcgaga gcatcaacaa ttcgtacgag aagaggagcg atcgtagtgc
   115861 gaaggatatt gcgatgtctg gcttgacagt ctttatatct ggccggtaga atcccgcttc
   115921 ttccgacgga cgcgggaata gctcagtggt agagcatcgc cttgccaagg cgagggtcgc
   115981 gggttcaaat cccgtttccc gctccaattt t
//
LOCUS       DAOQDS010000002        97136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_64_length_97136_cov_19.205797, whole genome shotgun sequence.
ACCESSION   DAOQDS010000002 DAOQDS010000000
VERSION     DAOQDS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 97136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 97136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97136
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64_length_97136_cov_19.205797"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1667)
                     /locus_tag="PK589_00620"
     CDS             complement(117..1667)
                     /locus_tag="PK589_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNJ18120.1"
                     /translation="MDDGPIPPEQRQSMRSWIFPALLLSAGILIGIVLTSDLGWLPTG
                     HAVPEAPAVPAPVPRPVSTAVQPTLPGAGGQSFVDVAKIVKPAVVNIFATRNGGGEEG
                     KGTPFEDPFFRRFFGEEWMKRFEAPKERKQQGLGSGVIVDANGLIITNNHVVNKADEI
                     KVFLSDKREFKAKLVGTDAKTDVAVLKIEATGLPTVAWADSDKLEVGEFVLAVGNPFG
                     LTQTVTLGIVSALGRAAGIAEYEDFIQTDAAINPGNSGGALVNVRGELVGINTAIYSQ
                     SGGNMGIGFAVPSNMAHSIMDQLVQHGKVVRGWLGVSIQELTPELSSQFGVPKEVKGV
                     LVSDIMDDSPAKKAGFERGDVIVEYDGKPMDSPAHLRNAVAQTVVGKKVTVKLIREKK
                     PKSIDLVIAEQPKNLSQAAAEEGGESIAPAGLLADIEVREVNSELAGRYGLKSSDRGI
                     VVVRVKPGSQAEEAGVREGDLVLEVNRKSVGTLKSYEHVAANLPKDQAVLLLLKRQGR
                     AIYLTLRP"
     gene            complement(1691..2614)
                     /locus_tag="PK589_00625"
     CDS             complement(1691..2614)
                     /locus_tag="PK589_00625"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNJ18121.1"
                     /translation="MSNRVQEILSWYESDNAGTKTNIARLLNSGKLAGTGKLVILPVD
                     QGFEHGPARSFAPNPGGYDPHYHFQLAIDAGCNAYAAPLGFIEAGASHFAGRIPLILK
                     LNNHDVLHDEKDPLPSVTGSVQEALRLGCSAVGFTIYPGSSHCNPMYEQLRAIAEEAK
                     NAGLAVVVWSYPRGSGLSKEGETALDVVAYAAQIAAQLGAHIIKVKLPSAHLEQAAAK
                     KVYEAEKIPTATLTERVKHIVQSSFNGRRIVIFSGGAKSDEKTVFEEVRAIRDGGGFG
                     SIIGRNSFQRPKAEAVKFLNTIMSLYAGEKP"
     gene            complement(2651..3643)
                     /gene="fbp"
                     /locus_tag="PK589_00630"
     CDS             complement(2651..3643)
                     /gene="fbp"
                     /locus_tag="PK589_00630"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNJ18122.1"
                     /translation="MAKFPITLSRFIIEQQAAHPEATGEFSVLLTQIGLVGKMIAHDL
                     RRAGLNKILGTTGETNVQGEIVKKLDQIANDTFVRVFEHSGLVCVLASEEMEKPLKMV
                     HEGAKYMLLVDPLDGSSNTDVNMPLGAIFSIRRSRTGQSVEDELLQKGTEQIAAGYVL
                     YGASTMLVFTVGQGVHGFTLEPSIGEYLLSNENIRIPSKGKVYSVNEGNYHRWPAGTQ
                     RYLDYLKVQDKPSGRPYSGRYSGCLVADVHRILLGGGIYLYPGEKDKPEGKLRLMYEG
                     NPLAMVVEQAGGKALTGTMRILDVEPKALHQRVPLIIGSAEDVSLAEDYVQGRA"
     gene            complement(3776..4588)
                     /locus_tag="PK589_00635"
     CDS             complement(3776..4588)
                     /locus_tag="PK589_00635"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNJ18123.1"
                     /translation="MTILRFPLSSTLLFVAAIVAGILLPSMLCAESTPDRRPSVSAPL
                     PFANGERLAYDVTWLGMRAGIATMIVQEGLDQRGRRQLTLSMTARSSPTVTKFYPVDN
                     RGVSQVDLDSFLPTHMTFARREGKRFNDFDYTFRHHEGLVTAVRDGKTDELQIPPDAQ
                     DAMSCLYYVRNVLPFVPGETLTMTVHHDKKNYKMDVRVEALETVEGRWGKAETARVVV
                     IMPFQGIFLNEGNIRVWFTTDPHRVPVRMKAKVVVGSIVAELTEGYGLAAQP"
     gene            complement(4590..5990)
                     /locus_tag="PK589_00640"
     CDS             complement(4590..5990)
                     /locus_tag="PK589_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNJ18124.1"
                     /translation="MALFREYDLRGIVGEELTEPIAEQVGRAYATMAREQGASRISVG
                     RDGRLSSPALQQALLRGLLAGGLDVVDLGLCASPLLYFSLFTLPVHGGIMITGSHNAA
                     EYNGFKICIGKEAIHGEEIQQLRSIMERGRFSSGSGQLSSHPIIPDYLQHLKTVFAGV
                     RADHLHVVIDCGNGAASLVAKQALEQMGCRVTGLYDELDGRFPNHHPDPTVVENLQDL
                     IATVREHKADVGIGYDGDADRIGAIDERGEILWGDRLMIVYARDILAQRPGTTFISEV
                     KASQCLYDDIAAKGGRPIMWKTGHSLMKAKLKAESAVLAGEMSGHMFFADRYFGFDDA
                     IYASCRLVEILAKTKHSLSSLVADLPQTTVTPEIRVDCPDTVKFQLVEQVHRQLASYL
                     EAGQPVGASNLRMRELVTIDGVRAIFEDGWGLIRASNTQPALVLRFEAPSPARLDVIR
                     ATIETELAQARRVLSV"
     gene            complement(5999..7447)
                     /locus_tag="PK589_00645"
     CDS             complement(5999..7447)
                     /locus_tag="PK589_00645"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNJ18125.1"
                     /translation="MTNHLYPVILAGGSGTRFWPLSRHLYPKQLLRIIGDETLIQQTM
                     RRVLSCASPDRVLISTNPGQADSIRVQLGEWKSDLKDNYVIEPIGRNTAPAIALVAAE
                     LLRRDPEAMMLVLPADHVVTGEKAFQAAVTLGMQLAEKGHLVTFGIKPTRPETGYGYI
                     QPNRRTTLAKKDRLTGHPVARFVEKPSRTKAAQYLKSGNYFWNSGMFLWKAATIRDEI
                     NRHQPQLAKAVQKVHTLMTSGADPRQIEAAYKKVPSVSIDNGVMELSSHAAMIPVGFG
                     WSDVGNWSSLEEVAPRDKAGNVVSGRVIDMDSSNSVLYADRRVVATIGLTDMVVVDTP
                     DATLICPKSRSQDVKQMVEILKQQGAPEHLEHVTVFRPWGSYTVLEEGPGYKVKRVTV
                     NPGGRLSLQLHHKRSEHWVVITGTARVTRGDDVLHLQIGQSTAIPVETPHRLENPGTE
                     TLHIIEVQNGPYLGEDDIVRFQDDYGRLGRAR"
     gene            complement(7496..7963)
                     /locus_tag="PK589_00650"
     CDS             complement(7496..7963)
                     /locus_tag="PK589_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNJ18126.1"
                     /translation="MRLFATDVDGVLTDAGMYYSESGDEWKKFNTRDGMGIKLLQKAG
                     IVTAIITQESTKIVMRRAQKLTIPEVHQGAYDKLAVLKDLIARHGLTMKQVAYIGDDV
                     NDLQALGAVGFSASPADGMPPVLKVVRYVCKKNGGQGAVRELADLILAAQKSQ"
     gene            complement(8122..10017)
                     /locus_tag="PK589_00655"
     CDS             complement(8122..10017)
                     /locus_tag="PK589_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNJ18127.1"
                     /translation="MKRIAVQVFQKVSSRFGQTILAYRTALIICVQAGLILAANFTAF
                     AFRFDGQIPALYKDTMWRGMPLVCVVYLLGLWGFGIHRGLWRYVGLHDLGRIMWACVV
                     STIVTFVLLHPILGRVEYPRSVILGTGLLAGLNIAGIRLLVRWFREWLQIVGPTARRV
                     LIVGAGNAGELLVRNMQMNPAYNYRPVAFVDDDPVKQGAKIHGVPIAGCIQNIREIVR
                     RFEVHEILMAIPSAPLALKQKTLAAVEGAKVPIKILPSVRQLLEDPALLQQVRPMSLE
                     DLLQREPIQTDRQELHPLIEGKTALVTGAGGSIGSELCRQIARYRPATLVLFERYENA
                     LHALLLELQAAFPDVCILPIVADVTMPERVAEVFRQTQPDIVFHAAAHKHVPLMELNP
                     KEAVRNNVWGTRVVAEAALASRVDRFVLISTDKAVNPSSVMGATKRIAEHVIQGLSQL
                     GQTRFTVVRFGNVLGSNGSVVPLFAEQIRKGGPVTVTDREIKRFFMTIPEAVQLVLQA
                     SVMGQGGEVFVLDMGEQIKVADLARNMIVLSGFVPDKDIEIVYTGLRVGEKLYEELFE
                     AGEQVLATAHGKINRAVGAPLVAEGFERWIAELHSKLAESDEEELLHDLKRIVPTFQP
                     TSPLRVS"
     gene            complement(10078..11400)
                     /locus_tag="PK589_00660"
     CDS             complement(10078..11400)
                     /locus_tag="PK589_00660"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNJ18128.1"
                     /translation="MKISRIPAVWASMEANHGPTLDLIVRALLYVYIFSLPFLPLLVV
                     ERNIFLVLLVLLPLWCLLRRRHFFVSSPVDLPLIAFVAWVAFTIPFSAHPAYSMKEFG
                     KLLQQVVIFYAVLYFFRERTARDLLTYLLLGCVLVVSAYGITQFQWYDGQATRSFFPA
                     EVWLTTYLVLLGPLFFSLAYYEVTNRLRYVYGCGSILVTICLLMTRSRAGLVALFVEL
                     WVMAWLLKRRSAVTVAGGATVVCLLAALFLIRVDTGSDGQLRLTLRDGTPVRTDGASV
                     IHRFDIWAFALSKIQEHWFVGIGYGKDNYKLVYGDEPEEVQPGHHPVKTAGAHNIVLA
                     SALHVGLIGMVLFLWLWGKLVRRLIQECHRTEGSTARAIVVGATVGVIGLGVRLMFDQ
                     MLVGTLAVQFWVVVGIAVLHVNSQRESDREGVVAAVSGAATLAGARSG"
     gene            complement(11390..12469)
                     /locus_tag="PK589_00665"
     CDS             complement(11390..12469)
                     /locus_tag="PK589_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ18129.1"
                     /translation="MALLGVGGLVLVLTYWGIGWLCMPGSRLYLLDYPNARSLHEIPT
                     PRTGGLALLVSAAIGLIVATLWEFPTQAWWLSASQTSPTAGAWILAMTVLLAVVSFWD
                     DRDGLPVLLRLAVQLIAAATVVIGAGVSLSSLSLPAVGSLSFEGVAVPLTLLFVLWMT
                     NLYNFMDGMDGFAGGMTVFGFGAIAYFAWQAQHPFILILSLIISMGAVGFLLYNLPPA
                     RIFMGDVGSISLGFLSGTLSVLGVQDGLFDIWTPLLVFSPFVLDATTTMLGRAVRGEK
                     IWEAHRTHYYQRLVLHGWGHRKTLLSEYALMALCVAAAVLYHGATDSGRLAILVVWIL
                     GFAGLAVGVGLMEQRAGQQGRMYED"
     gene            complement(12502..13449)
                     /locus_tag="PK589_00670"
     CDS             complement(12502..13449)
                     /locus_tag="PK589_00670"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ18130.1"
                     /translation="MNVLVTGASGFVGHAVCAQLVAQGVTVTAAVRRPAAQPVRGLAY
                     CQVRDLGPDTDWHEALTGVHVIVHCAARVHVMQETDADPLPAFRTANVCGTVALARQA
                     AELGVERLIYVSSVKVNGEGGQDAYRETDVPAPQDAYGVSKYEAEVGLREIAAHTALK
                     VVVVRPPLVYGPGVRANFQLLMQALARGIPLPLGAIRNRRSLVGLGNLVDFIVTCIEH
                     PRAANEIFFVSDGEDLSTTELIDRLALAMNRPARLIPVPMPALRSGLTLLGQREMAAR
                     LCGNLRVNITKARTLLGWCPPIRVDEELRRAAEYYLQHQ"
     gene            complement(13446..14567)
                     /locus_tag="PK589_00675"
     CDS             complement(13446..14567)
                     /locus_tag="PK589_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ18131.1"
                     /translation="MSRCPKLLFVVTEDWYFVSHRLPLALAAQKAGFDVVVATREGEH
                     GERIRAEGIRLIPCHLSRRRGNPLQEIAMLARLYRRERPDIVHHVALKPVLYGALAAK
                     IAAVPHVVNAVAGLGWLFTSNTLAVKALRPAFCRLLVWLLGASSSCTIVQNPDDFGLL
                     TQKGVPEDRLRLIRGAGVDTEVFAPPSQLPSEPVTVVFAARMLLDKGVREFVEAAHVL
                     TDAGVKARFVLVGDPDPGNPASVPDTSLRAWHGQHGIEWWGRREDMPAVLQLGHIACL
                     PSYREGLPKVLLEAAACGLPLVTTDAPGCRDVVHDGDNGFLVPIRNAQALALALARLI
                     EDAALRATMGRRAREIVLAEFSQERIIKETLAVYLELTP"
     gene            complement(14564..15037)
                     /locus_tag="PK589_00680"
     CDS             complement(14564..15037)
                     /locus_tag="PK589_00680"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNJ18132.1"
                     /translation="MIFAALLWSIVLLVPQKYGNMSVQSKLSYESSNLERWETLTHGL
                     EMWQQSPVLGVGLGVFNEKSPMWAGHPQVIHSTPLWILAEFGLLGGAVFGWTFFLLAH
                     HAIRSGTTQPANCILLLLLVAFAVFSLAHEIFYQRILWLVLGAVLTRPYAHRRHA"
     gene            complement(15034..16728)
                     /locus_tag="PK589_00685"
     CDS             complement(15034..16728)
                     /locus_tag="PK589_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNJ18133.1"
                     /translation="MLSNFSFRRLPAVFVSIFLIAFVLWSAGVEDVLRRLAGFPPWAV
                     ASMLALLTANLFVVSFRFWRVLAHFGIALPWDIALRASIAGTVAGLFVIPLFGQIMGR
                     QGVLNSFGVQPVVNASLAAYERALLALLSGVLGTLGAVYLLGRSAVSDFFNQIALPEI
                     VVAAIGGWALSLWLGGSRFERKLSRRTLSWTNLEHVMSIAGLTLTGQLLMLACFVVGI
                     LAVSQDIPIASLFAASALISLAASMPITVGGWGVREVAAVYVFGLLGIPAADALAMSV
                     MVGLCSTLVILASSPFCFRNSLMSDLPKPPAVMIHSVSEIEEFTAWVLGTVVAVAVFF
                     QVHVSLSGGPINLNLADPFAILALAAVSLHALFARQFPVWRVPQFNFALCVISLLLLV
                     GFVHGWLEIGITQWALGGRLLGWLVLLGYLSSGYVIVANVGTHGLRRFAETLIATAVV
                     VVMLQVALRLLSHWGVNAAAQLTQNFEGYAGNRNAFALQVLAGMALFLGYSHLYARHN
                     IRVPRSVRPWIFSLLLGILLAGLVWTGSRTAMLVGAMALVLAWLGGMADHKMLGWG"
     gene            complement(16718..18691)
                     /gene="asnB"
                     /locus_tag="PK589_00690"
     CDS             complement(16718..18691)
                     /gene="asnB"
                     /locus_tag="PK589_00690"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNJ18134.1"
                     /translation="MCGIAGLLSQSRGELDATVARMVAAIRYRGPDDSGIWSDPAAGV
                     GLGHARLSILDLSPEGHQPMSSRSGRYVLSYNGEVYNFGELRTEFEAAGTTFRGHSDT
                     EVMLAAFDAWGIEMSVKRFVGMFAFAVWDNHTQTLTLGRDRLGEKPLYYGWQGQTFLF
                     GSELKSLKAHPAFRAEIDRDALALLLRHNYIPAPYSIYQGISKLVPGCLLTVSLSQRT
                     PQTKAFWSSRQCVEAGLAHPFEGSEAEAMSRLEALLKEAIGRQMVADVPLGAFLSGGI
                     DSSTVVALMQAQSSRPVKTFTIGFHEEGYDEATHAKLVARHLGTEHTELYVLPKEAMD
                     VIPRLPTLYDEPFSDSSQIPTCLVSELARRHVTVSLSGDGADELFGGYNRYFWATTIW
                     RRIGWAPQSIRTALAGALTALPPSAWNSVFQRFARFLPEGWRYANPGDKLHKLAETLA
                     VATPEEIYFDLLSHWKHPAEVVRGAHEPPTVLTDQTQWANLPDFERRMMYLDQMTYLP
                     GDILTKVDRAAMGVSLETRVPFLDHRVVEFAWSLPLSMKIRDGQGKWLLRQMLYRHVP
                     KALIDRPKMGFGVPIDVWLRGPLKGWAEELLDESRLQREGYFDPQAIRTKWLEHLTGR
                     RNWAYYLWDVLSFQAWLESMGDSAHNGAHILAQ"
     gene            complement(18730..19875)
                     /locus_tag="PK589_00695"
     CDS             complement(18730..19875)
                     /locus_tag="PK589_00695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ18135.1"
                     /translation="MIRIIHVISGLDTGGAEVMLVKVLQGLDRTRFTNIVISLTDRGQ
                     LGEQIEASGIALHTLGMKRGCPNLSALVRLRRLFTTLQPAIVQSWLYHADLLSTLAVK
                     VSGSPILIWNLRCSHMDLTGYSRLTRIVQWMLVWLSHVPVAVIANSVAGRQQHARLGY
                     RPRRWVVIPNGFDLQRFRPDRSARAAIRTEWGLSDDTVLVAFIARVDPMKDHATFLDA
                     AKRVVDAKKKAHFVLVGKDTETLAPSVAKRGLTDHVLLLGHHGHIERLLPGVDVGCLS
                     SLGEGFPNVLGEAMACGIPCISTDVGDARDIIGDTGLVVPLRDPAALAGAMTDLIDRG
                     PAGRAKLGYAARARIEAEYALPKVAECYEVLYTELAAQSRSPRERMC"
     gene            complement(20042..21166)
                     /locus_tag="PK589_00700"
     CDS             complement(20042..21166)
                     /locus_tag="PK589_00700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ18136.1"
                     /translation="MKVRFLIRSLQRGGAERQCVALAKGLRQLGHDVGVLVFYSGGDL
                     YDELLAAGIPVESVGKLRRWELVGFLQRIRRCLRRERPDVVYSLLQVSNVVAAVEKLF
                     VPTLRVTWGIRTASMDLTHYDRLVRVSYWLEARLSRFADHIIVNSEAGLRYAKVEGYR
                     TDRISVIPNGIDTELFAPDRSKGKTVRCSWGIRDEEVLIGLVGRLDPIKDHPTFLRAA
                     ALVVRQCALVKFVCIGGGDSAYKESMVALCAELALSARVIWAGEHSDMPAVYNALDVA
                     CLTSTAEGCPNTLLEAMACGVPCVATDVGDSKRILGEIGGIVPSRNSEALAGAIQELL
                     RMDGAERAKQGQMGRDRVIQHFSLDRLIGDTASVLEATAR"
     gene            complement(21163..22005)
                     /locus_tag="PK589_00705"
     CDS             complement(21163..22005)
                     /locus_tag="PK589_00705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ18137.1"
                     /translation="MMTQQAQEVADGRRFEFGQNWRRFLEDLTEERIGRAQESLQAML
                     GLKSLAGQRFLDIGSGSGLFSLAAKRLGASVRSFDYDPHSAACTAELKRRYFRDDDTW
                     IVEEGSILDMAYVQSLGTYDIVYSWGVLHHTGAMWQALDNASRLVADQGTLFIAIYND
                     QGGASRRWLMVKRVYNRSPQWGKILVAWLACIRQWTLTVVRDTFLGNPLRTWFSYKEQ
                     RGMSPWRDVIDWVGGYPFEVAKPEEIFDFYRKKGFDLQRMKTCAGGIGCNEFVFHRVS
                     TERL"
     gene            complement(22019..23113)
                     /locus_tag="PK589_00710"
     CDS             complement(22019..23113)
                     /locus_tag="PK589_00710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ18138.1"
                     /translation="MKVTFVISALFAGGAERVISIMANYWARKGWGVTILSFDDGETP
                     PFFEVDPSVRRMSLGLLGDSAGLIAGIRNNINRVVRLRQAILRSQPDAVISFVDKTNV
                     LTLFATLGLKLEVMVSERTNPYQYSIGRIWGALRWWVYSRADRIIVQTHAARAYFLPK
                     FQRTVSVIPNPVLPSSSNYAMVNHRPAGCMILAMGRLSKEKGFDDLLYAFRKVNDQHP
                     GCTLTIIGEGPERGELELLCERLGLSGCVLLPGLVTTIEVFLRQASMFVLSSRFEGFP
                     NALCEAMAYGLPVIATDCRSGPREIIRDGEDGLLVPPGDRDALATAMDSLMSDPERRA
                     RLGGRAMDITQRFGVEKVMGMWEKILEPIA"
     gene            complement(23171..24433)
                     /locus_tag="PK589_00715"
     CDS             complement(23171..24433)
                     /locus_tag="PK589_00715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ18139.1"
                     /translation="MAEGHAELTSADPISVVHLIDGLGGGGSERWVWDIVRLSEPTLC
                     KHFVISFFPDNAKFVYSEPLRSRGAYKQPAHHPFLEWLERGIQTVASQWYLLPIRKTL
                     SGLWWFCNAVDVWWRVAVALVQKRPDVIHVHTFHGLRVGLVLRRMLQIPLVHSVPALF
                     SQMTDAGFHGIPQLYRSNQQWVARFITGASVAELHSIGIPDSKIMPIHGTVDLTSSTS
                     MRKQKQHYYTALRDELKLPGDAVIALSVGRLHPSKGHAFALEAVPELVAQFPQLHWVV
                     LGEGEQREALEARASELKVREHVHLLGFQRDPLPYYAAADIYLRTPVFESENLSSYQA
                     MAMGLPVVGFDTGCETELLGKSGHGVLVPARNSEALAKAVVEMLSLPDRGARIGLRGV
                     EYCRQHLDIDRTISDLVRLYQHLGAKRA"
     gene            complement(24409..25731)
                     /locus_tag="PK589_00720"
     CDS             complement(24409..25731)
                     /locus_tag="PK589_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNJ18140.1"
                     /translation="MSTLKLSLVGLGARVILLVINVISVVLLARYLEPQGRGEYFLFQ
                     TLVSVLSVVGDCGLSQSANVFVGRSAHRKKLIHAFVSKAAIILWIGMSGIGGLVLWMA
                     GEQLLHDFPARWQWAAFAILPCMLYAGFWNSLMIGLGEIPLLNGVQLVIGPVQLILIL
                     VLVVGFSGGVASAVTIYLSTMVLQCVLMVALSRRLGFLGGSEYVEAGLSRQMLAFGLR
                     AYPNAIATMGWMRIPVFVLNAFHGAASVGIFSVAQQLAERALMPIQAAQDAIYGKMSA
                     LSPREAIAAMNRYIRIVVSGMVASTVGAMLLAPWIVMALFGPSYQPAVYVFWILLVGV
                     VFVSVSMIVSTYVLGQRGRPGLLSLLAGANACICLASSYWLIPTWAEMGAAMALALTQ
                     AVGAVVVFGLYWITSSATLRETLLLTSEDVTSVLKQLGKLTWRKGTQS"
     gene            complement(25847..26980)
                     /locus_tag="PK589_00725"
     CDS             complement(25847..26980)
                     /locus_tag="PK589_00725"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNJ18141.1"
                     /translation="MGIPFYRHDLGEPEVEAVRKVLAGPILTTGEVVESFERRFAAYL
                     GVPHALAVTSCTGALHLSLLALGIGLGDEVITTPMTFIASSTAILEAGAKPVFVDVES
                     QTGNLDATKVEAAITPRTKAILPVHLYGQMCDMVTIRKIADQHGLFVIEDAAHCLEGV
                     RDGVKPGELGDTACFSFYATKNLTCGEGGALVTKDRGLAEKLRLLRLHGMTKTAADRH
                     REGYQHWDMTVLGWKYNMDNIHAALLLPQMDRIEQNWSKRDTVARWYEGLLSDCPGVS
                     VPKSHPQVRHARHLFPVFVEGGGRDAIIQGLQEDGIGSVVNYRAIHLLSYFRDTFGFR
                     VGDFPHAEKIGDSVVSLPFYPAMTENQVTQVTATVTRLLKARR"
     gene            complement(26982..28016)
                     /locus_tag="PK589_00730"
     CDS             complement(26982..28016)
                     /locus_tag="PK589_00730"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNJ18142.1"
                     /translation="MKTILITGGAGFIGSNFVRYLYDKYPNYRLIVLDALTYAGNVKN
                     LPVDINNGSDERLMFWYGNVRNAELVDTVVSQSDVVMHFAAESHVTRSIYDNLLFFET
                     DVLGTQAVANAVLKYRDKVERFIHISTSEVYGTAESEKMSEEHPLLPMSPYASAKCGA
                     DRLVYSYWQTYGIPAIIVRPFNNYGPYQHLEKVVPRFITSCILDEPITVHGDGSAARD
                     FIYVQDHCEALDLILHAEPKRVFGEVLNLGTGRHISLMEIADTVRALMKPKKSPIQFI
                     GDRPGQVFRHTCDHSKVSRILGWQPRTSFEQGLERTIQWYRENEAWWRPQMWMRRIPI
                     VTAAGKRELH"
     gene            complement(28030..28740)
                     /locus_tag="PK589_00735"
     CDS             complement(28030..28740)
                     /locus_tag="PK589_00735"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNJ18143.1"
                     /translation="MHDPNLQSSITLCVLQPGYLPWLGFFDQMRRSDIFVLYDDVQYD
                     KHGWRNRNRIKTPKGPHWLTVPVRHKGLGWPRINEVEIDNQTVWGKKHIGTIRQFYAK
                     APYLAQYLPMLEDRLLRPWTLLVDLDLSLIDLMCEWLQLKRDVIRASSLGIEGDRSDR
                     LLALCRHFGATRYLSGDAAKTYLDRELFAQNGIEVEWQGFQHPVYPQQHGEFVPFLSA
                     LDLVLNCGPDSAAILRGQ"
     gene            complement(28792..30648)
                     /gene="asnB"
                     /locus_tag="PK589_00740"
     CDS             complement(28792..30648)
                     /gene="asnB"
                     /locus_tag="PK589_00740"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNJ18144.1"
                     /translation="MCGIAGRFGPGPNQAVKKMVALLAHRGPDDEHVVSGRNFAIGAR
                     RLSILDLQGGRQPISNERETIWAAQNGEIYNFPALRNELLERAHVLKTHCDTEVLPHL
                     YEEHGAAFPKKIDGMFAVALWDGDGQVGYLARDRTGKKPLYYCLHDQALYFASEIKSL
                     LQIPGFTRRLNLDALHQFLSLKHVPHPLSIFEGIAMLPPAHMLTYRAGQEPVVSRYWD
                     LSFAGSDTSAGMTEEEITDRLLVLLRQGVERRLLSDVPIGFFLSGGIDSSLSTVLAAE
                     LSPSKIKSFTLTYSRESTTEGKEEDRRWARWVAEQYGTEHHEETIQVSSFPENLRRIL
                     TCFDEPYAGVVSTYFLAQLMSQHVKVALSGDGADELFGSYLSHRLAVPLANFQTYLQT
                     GNSALIRPFEQQVDFLARMADPQDWGWRYKLLVFSDEEKDALYSEEIASHMRSYSTRD
                     RLRHDFSELSARDPLNRILESEFRTIFPDQVLAFVDRLSMAHSLEVRTAFLDTDFVEF
                     VAGLPGSLKIKDGETKYLLKKLGLRYFPREMVYRKKEGFLMPITQWLLRDLESYVRET
                     LSPERLAKHGLFRAAYVQRLVDALYQAQPDHLAVNKVYALVVFQEWYDLYMV"
     gene            complement(30654..32147)
                     /locus_tag="PK589_00745"
     CDS             complement(30654..32147)
                     /locus_tag="PK589_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18145.1"
                     /translation="MEMFFAPQGFLHPELSLSVQMFMRVVYGMLLCGMLLLAIPNWRR
                     FFLSERWGGYAQSLREVEFIQNPLVFPLVMAVWFACGVCITGGWWTPWPALLNLVLCR
                     YFFVGMRWKGLLRGMGAPGYILYWGGLAITVLEYTLHYAPELRSLALQVLQADFACVY
                     ISSGVYKLSAGYAKNHGMELGMTNPAWGYWWKHYQHFSPSHPLFRFLNHLAWSSQIIA
                     GILMLLPPTRFIGGMIIFGMFAFIATQIRLGLLCEIVMFCSSLIFLHPGSLGDIVVTA
                     VVGQVQSPSVASFAFSSVANTVLGWALAIYLILLPFAHVGLSYNFYLRRSLPKPLQMA
                     LERYTNFFGLIVWRVFSVDIVNFFIRIYHYPKAHESDRQLISDESVGARLRFGHVAES
                     IVLACIFTTLKYYPHNSGLFHERLLRYARTFPCEKGELLIFEYLSLRKEPDRFVFAPV
                     AEYTVDTAAMTVVERVIDPSVSVRAPHAVSPVHEGVRPGTYAPAGGR"
     gene            complement(32219..32653)
                     /locus_tag="PK589_00750"
     CDS             complement(32219..32653)
                     /locus_tag="PK589_00750"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HNJ18146.1"
                     /translation="MSMKERVYVIYDGQCRFCIRSLKLFSAADIFKVFRFYDAHDEQS
                     VAALFPELQGADFDNAMFVVTERREVYRGFFAFRRMIWSSPLLWPLIPVFYFPGATPV
                     GTRLYAWVAKNRLKFGCQSDACTLPARLPKDPSSDHVDVVPH"
     gene            complement(32677..33636)
                     /locus_tag="PK589_00755"
     CDS             complement(32677..33636)
                     /locus_tag="PK589_00755"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNJ18147.1"
                     /translation="MSEKNVVQNKAEAHRGRVIVFGYGALAAAAVTALLSAGAEVIAV
                     VAPSNRSGDDVDFIKDFACTAGIPVLVQPPRKTIAPFVQDLKRLDPDVIVVWSYPMIL
                     PPDVIEVPREGTVNVHGGLLPEYRGGHVMQWAIINGESETGVALHYMDEGIDTGPVIA
                     QRRFPIERDDDAATVREKLKEAGMALLKEWWPAISQGRAPREKQDEATAKYHRLRTID
                     DGLINWHASATEVCNLVRALVRPWPGAFTILNKKKLVIWKCRLLVQETSGVPGLVVQV
                     DAQGARVCTRDRDVLIETAELDGERMTGLDLKRIMQAGTCLGA"
     gene            complement(33665..34783)
                     /locus_tag="PK589_00760"
     CDS             complement(33665..34783)
                     /locus_tag="PK589_00760"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ18148.1"
                     /translation="MTSKPLVVVPDEKTYGLKPGAEEFPLMVIISIIYPCNMGCPNCP
                     YTDTNSELRRFYHEHDGDLIPVKLWEKIADECGPYGAWMRCTGGGEPMLHPKMVEMIE
                     YAKAKGARVWLNTNGSMFGPNAKHRERLERVIRAGIDLIEFSMDAADAETYSVLRPPR
                     SGKTGDPEQWWTKHVGNVRAALDLRKQHVATTRVVTSIIRQEAIEGRLDDAVQFWLKD
                     VGVDEVITRKFLSWDDNTTIQLGKTLDRHLYADLPKQKKEPCVWPFERLNVDTLGRIA
                     LCGQDISFKTSELFPNVKDTSIKEIWQGEMFTWYRRMHLEGRGAECFPCRGCSAWFAG
                     IRDWDHGWQKVLRNSGDHLKEVMRRDLGVEVEVFQPKV"
     gene            complement(35077..36015)
                     /locus_tag="PK589_00765"
     CDS             complement(35077..36015)
                     /locus_tag="PK589_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNJ18149.1"
                     /translation="MEKQARIFVAGHQGMVGSAILRALTSRGYERLIVRASGELDLRD
                     NVRVAAFFEEVKPDYVFLAAAKVGGILANSTLPAEFIYDNLAIQTNVVHQAYVHGVKK
                     LLLLGSSCIYPRDSPQPMKEDYLLTGPLEPTNEGYAVAKIAGIKMCQAYRQQYGCDFI
                     VAVPTNLYGPNDNFDLQHSHVLAALLRRFNEAREQGGEPTLWGSGTPLREFMHADDCA
                     DACLFLMERYSHAEIVNVGAGEGISIGRLATLVAEVVGYRGDIHWDPTKPDGMPRKLM
                     DCGRMKALGWTPRIPLREGLQDAHRWYQGHVKRIAD"
     gene            complement(36515..37372)
                     /gene="rfbD"
                     /locus_tag="PK589_00770"
     CDS             complement(36515..37372)
                     /gene="rfbD"
                     /locus_tag="PK589_00770"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNJ18150.1"
                     /translation="MRIVITGAQGQLGKELQQVLQGHQLTLLDLPTFDLTHEDCGRLI
                     VDAVPDAVIHAGAHTDVDGAERNPQLAMAINADGTERVARAAAQVGARLIYISTDYVF
                     DGRGTRPYVETDQTNPVSAYGASKCAGEARALACCENTLVVRTAWLYGLQGKNFVKTI
                     LQLAAERPSLRVVADQEGCPTFAEDLARMIDRLVTHPIRGFLHVTNEGHCTWHEFAAE
                     IVKLAGYRVPVQPITTEDMPRPAKRPAYSVLSSDRLHHLGFSMPSWQNGLQRFLKALP
                     ALSSMSSRT"
     gene            complement(37357..38388)
                     /gene="rfbB"
                     /locus_tag="PK589_00775"
     CDS             complement(37357..38388)
                     /gene="rfbB"
                     /locus_tag="PK589_00775"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNJ18151.1"
                     /translation="MRILVTGGAGFIGSHLVRRLVQSGRHSVVNLDALKYSGNLENLT
                     DLVDHPQYRFIHADIGDQKAVHAALQEHRIEGIINCAAETHVDRSILDPGAFARTDVV
                     GTGILLEEARQAGVQRFLQVSTDEVYGSVEQGSSTEGDRLEPRSPYSASKAGGDLLVL
                     SYWTTYRFPVVVTRGSNTYGPNQYPEKFIPLFATNAIDGEPLPLYGDGKQCRDWLSVY
                     DHAAGIQHVFEDGQPGTVYNVGGGNERENITVAEQIVAALGKSRSLIRFVQDRPGHDR
                     RYSIDCSRLRALGWSPQVPFEEGLQQTVEWYRTHEDWWRKIKSGEFKAYYQQQYGQRL
                     AKGTACGSS"
     gene            complement(38388..39122)
                     /locus_tag="PK589_00780"
     CDS             complement(38388..39122)
                     /locus_tag="PK589_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNJ18152.1"
                     /translation="MKGVVLAGGLGSRLLPLTKVTNKHLLPVYNKPMIYYPIQTLVNA
                     GITEIMLVTGGNSAGDFLKLLGNGRDFGLKHLSYTYQQGEGGIADALRLAEHFADDGP
                     ICVVLGDNLIQGNIAKAAEAFRTQKKGAKILLKEVKDPQRFGVPHLDGNQVLSIEEKP
                     AHPKSSYAVTGIYFYDARVFEITRTLKPSGRGELEITDVNNAYIASGELTWNVLDGWW
                     TDAGTIESLLAANQLVAQTGANRMEF"
     gene            complement(39269..40351)
                     /locus_tag="PK589_00785"
     CDS             complement(39269..40351)
                     /locus_tag="PK589_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNJ18153.1"
                     /translation="MKIVIAAWHLKNFNVGIGRYARELIEALGSVDQTNHYDILLPQP
                     EHPFTARPNMRYRVVRFPLFRRRFWEQVAPLMVGPYDVLHFPYDSCVAWKRGKFVTTI
                     HDVKPLLFPELRPRTNLNSRIEDWLVGDRWQTIDQVITVSEHSRRDLLAHVPLRPDQV
                     SVTPLGLDAGRFRPAEHRHEGRPYVFCVAGSDPTKNVGVLVEAFATLPADLRNRFDLI
                     LAGDVCKREDIRAAIARRGLGAQAKLIGLLSDAELVTYYQQATVFVFPSLYEGFGLPV
                     LEAMGCGCPVICSNASSLPEVAGEAAVLIDPRRTDQLTQELARVLESIELQLSLRERA
                     LARSKEFVWERTAKQTVAVYERAVGR"
     gene            40531..41406
                     /locus_tag="PK589_00790"
     CDS             40531..41406
                     /locus_tag="PK589_00790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNJ18154.1"
                     /translation="MAHVSVVIPAYNGTSRYLNEAIRSVQAQSFADYEIIVVDDASTD
                     RTDALVQAIPQVRYVRHSKNAGQAAARNTGAQLAQAPYLAFLDQDDLWEPTFLERTLA
                     ILEPATGAVLVHCDGYQVNERNDILEYDAAMKHQRSITQLLRGGHDAATSGSLFRKSW
                     FDSVGGYDAALSIWEDIDLAIRLYGPERFIHLPEPMYRHRLYGHNASRDIPSLRALDG
                     RRRFLEKHAATCRPGTPEGEALSNDWAIYYADLGKHHLRAHARQEARQAFRFALRHQP
                     LNFKTWSRLLRSYFV"
     gene            complement(41387..42424)
                     /locus_tag="PK589_00795"
     CDS             complement(41387..42424)
                     /locus_tag="PK589_00795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ18155.1"
                     /translation="METIDCEVCGGTSTTTMFSQYDLTHRVTDDVFTVVRCLDCRQLF
                     LNPRPTRQEIGRYYPDTYYPDAAPRQAGDLRRAAKRWSATIRRWIAQDFYGYPAQEQG
                     RAWQRVRRMFLWPEFLWRCWRGRGLLPWVGQGRVLDVGCGAGGNLASLQEQGWEVSGV
                     DASAVAVTQAKACFGDRVRLGDLEAIAYPDRSFDTVLFSHVLEHLHGPLPLLKEVWRI
                     LDQDGRIVILCPNAGSLEAKIFGRWWFPWELPRHLFHYEKATLRRILEAAGFRIESMR
                     TGLGSLYFMASLDRVCEQRFGRAIPCRRLLEKSLIRPFCFCVGHLGYGTELKVYARKD
                     ASLNQATQSTI"
     gene            complement(42456..43352)
                     /locus_tag="PK589_00800"
     CDS             complement(42456..43352)
                     /locus_tag="PK589_00800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNJ18156.1"
                     /translation="MNESVSVVIPVFNGAPFVAKAVASVRAQAHGDVEILVVDDGSTD
                     GTQEVLKRLEESDGIRWFQRSHGGPARSRNFGIEAARGQYIALLDCDDVWLPGKLAAQ
                     LAIMRTRPEVGLVHTDFDVRFEDGTLEERVIARSSREPMVQAFAGGHVALPSTLLIRK
                     SVLDQVGRLDPELYGSEDSDLTIRLFRVTSFECIDQVLVTKLQRGHGYRDMAFDEQTH
                     RERVLASRDRFLMRLEGFAPLTTAQRAALDREWANYYLLKGAAAERAGRPAEARSQYR
                     KAIGKAPGRIRCYTRWLRTWTS"
     gene            complement(43349..44407)
                     /gene="rfaQ"
                     /locus_tag="PK589_00805"
     CDS             complement(43349..44407)
                     /gene="rfaQ"
                     /locus_tag="PK589_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HNJ18157.1"
                     /translation="MIATGLLRPKNILIIKLRHIGDVLLSTPVLRALREAFPAAQLTM
                     LVNRGTEGVLAHNPDVNEVLCLEKGAWADQCKFVYRLRERGFDCVIDLTDGDRSAVIS
                     LATGAPIRIGFNAEHRWRGLLYSRVAKPRPVDQHRVEYDLCALRALGLDPKRGMPSVS
                     VSDSEEQVTDQWLTEQGLIGDQCPPLVLLQPGARYSLKVWPPERFAQLSDRLVEQFRC
                     RILLGGDQREREVAEQVARKSHCAPIVVAGKFSLLQYASLVKRCALFVGNDGGAMHIA
                     ATVGTPVVALFGPTYPERWGPRGGLTQVIYKGLDCRACYHPSCLRGDDSCMRQITVDE
                     VFEAAGRILDLKGARVVT"
     gene            complement(44404..45507)
                     /locus_tag="PK589_00810"
     CDS             complement(44404..45507)
                     /locus_tag="PK589_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNJ18158.1"
                     /translation="MRIGIDASSLVGDKGGVGWHTYHLLKALLQLDEKTDYVGYLRPG
                     SLQAGQLSGWPSRSRMQWMETPRWLMSWRGRWDRLDLYHGPNFKMHTTGRYGGIVTIH
                     DLWLARHPEYSRKLFGQAGSSRRAIATAKRARKVVTVSEFSAREIESLYGIPRDHVRV
                     IYNGVSEDFTPLCDDQIRAEVRTRWSIPSSGFILFVGGADPRKNHVGFLQAVGACRSQ
                     LGGRAIVLVGDAEHPQGSYTATAQSLGLEQEVRCTGRLDREDLRRLYSCADLFVFPSH
                     YEGFGMPVLEAMACGAPTVTSSTSSLPEVAGDAALLVDPNNVEELAHAMVTVLSDTHL
                     RENLRQRGYERARLFTWQRAAVSTSALYRELCT"
     gene            complement(45557..46318)
                     /locus_tag="PK589_00815"
     CDS             complement(45557..46318)
                     /locus_tag="PK589_00815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ18159.1"
                     /translation="MTIAAVVITKDEEHNIAECLQSLRWVDELIVVDAESRDRTVELA
                     KGYTSKVFVRPWPGYGPQKNFGIDQAGADWILVVDADERVPDALRQEIQALVDSRPPA
                     DIGGYEIPRRNVFYGKWIQGGGLYPDYQLRLFRKTAGRYDDVRLHENLALSGRRERLR
                     EPFIHYSMPTVNHHIRKMMRYTTLGADEKLKRVRHISGWTIATHHLGTILKTLFTRGG
                     YRDGVHGLVVAMFAGLHTFVKYAKAWERLNVRRDA"
     gene            complement(46349..47146)
                     /locus_tag="PK589_00820"
     CDS             complement(46349..47146)
                     /locus_tag="PK589_00820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ18160.1"
                     /translation="MDRIPVSAVVIAYNDEPNMRACLESITWADEIIVVDSHSTDGTE
                     RISREFTDKVYQHDFHGFGRLRNEALAHATHDWVFSLDTDERATPELRDEIRRLLDAG
                     PEADAYFVPRKNYFLGRWIKHCGWYPDYRQPQLFRKSKLRYRDELVHESFDLDGRLGY
                     MTAAALQYPFRDIDHYLAKQERYSDLMAKRMVEQGRAFSWHQLVSHPCFTFLKMYVGR
                     KGCLDGVPGLILSGLYAYYTFIKYARFWELQSQGVRQPNRVAPAKQG"
     gene            complement(47203..49344)
                     /locus_tag="PK589_00825"
     CDS             complement(47203..49344)
                     /locus_tag="PK589_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNJ18161.1"
                     /translation="MMLRWADSGASIRKSVLFWWILFVVIQMAERVFLLRDALDQETP
                     TASLLLKTLLVGVRGDFITATFAIVLGGVGAGLYALLRQGVAGLRQSPRRFSLSFRPA
                     FHVGCLLAGLLLFVLLCVDMGYYGFNHQHMDFVFLEYIGDLLAPAATTEGTNVQAMQQ
                     TSAELEAAGKWAWRLTVFLAIQAAGIGLWWWSFSRAVVPALDRWRPGSGFQANALLVV
                     ALVAGGAGFHHSGPYGIRIAPIGSMVYYTLAQNPVLFAAEALRIAVVSRNPIERRANT
                     EAMPYDLAVRTAQQLLGPGETFVDPRYPFVRTTSPSPDGVRLSKPANILLVFLEGLDR
                     RYLGQTYGEVKGTPFLDRLKQDSVYFQNFFSNGVQTSRGLFATLCSTFPRQGAAAMKT
                     RYAHDYLCLPSLLQRQGYSTEMVIGQHRDLNRLQTFVARNGLQQLLDEGDFPANTERA
                     GLGIVDGALFDLCYERIKLRQSQGKPFFLSTLTLSTHHPFAAPMNHPEVQLLQQQVQD
                     KYVAALRYTDLELERTFTRLVREGLLRNTIVVILGDHGRHEPVGGSDIERKVGHFASP
                     LFLWMDESLRTPATYRPRVVDTIASQVDVAPTLLSLNGGLPAMGSFAGHDVSCALVQD
                     CLPGQVAYLTSVYDNLVGLASAEGLLLYSFATETFQEATLRFEPVPDEQTGGRRLSVS
                     RDRELMALYEVANVALDSNRLWSWREFGSRL"
     gene            complement(49341..50444)
                     /locus_tag="PK589_00830"
     CDS             complement(49341..50444)
                     /locus_tag="PK589_00830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ18162.1"
                     /translation="MNILIAESSMAVGGQELAVLLHAERLVARGHHIRLVLEPRSPIM
                     AMAQERGLPVEPFVMRQWRLPLSILAFRQLLTRERPDIVHVNSSRDSWIAALSTRLLS
                     PRPKVIRTRHISAPLNNNATTHLLYRRLFDMVIVTGGERNRQDLIHRDGLSPDRVAAF
                     PIGLDVEQFSPAQPTHDIRAELGIPPRHLLIGMISYLRDYKGHRYLVDAAAKVLKQHP
                     AAAFLIVGEGPEEQNIRAHIERLGLAADVRMLGFRDDLLDVFRSLNLFVIPTVEGDTI
                     PQVLMQALAVGLPVVSTTTGSIPDVIADGESGFLVPPRDPEALADRIVRLLKDANLRA
                     AMGKRGRHTVEQSYSIDRMVDELERVYRKVIAS"
     gene            complement(50441..51484)
                     /locus_tag="PK589_00835"
     CDS             complement(50441..51484)
                     /locus_tag="PK589_00835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNJ18163.1"
                     /translation="MTEPVRRILLIKPSSLGDIVHAMPTLAALRERFPQAEVTWLVKR
                     QWASFVEVIKGVDHVCAVEQGLRGWLGRVPDLRAARFDLVVDLQGLFRSGAMAWLTGC
                     SRRVGFANAREGSPFLYTQRVAVPNGSMHAVDRYLLVAEALGAARQTDPRFDFADRAE
                     DRGAAEALLTAAGIRVSAPWIAMNVSARWETKRWPAQHFAEAADLLAEAQHIPVIFMG
                     GPAERSETRAVMARMRTKAVDLTGQTPVGLLPSLLRRAAVFVTNDSGPMHIAAAVGTP
                     VVALFGPTDPVKTGPYGQGHVVLSNPVECRPCFRRECSRAATLECLTGVTPEQVVRAV
                     QRQLGRLDTSGSS"
     gene            complement(51509..>52267)
                     /gene="gmhB"
                     /locus_tag="PK589_00840"
     CDS             complement(51509..>52267)
                     /gene="gmhB"
                     /locus_tag="PK589_00840"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:NF006506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HNJ18164.1"
                     /translation="RQPVSCAPCLLRECPIDHRCMTGVTVEQVHDAAARHLPLAAPPS
                     LIVPTVVKPALSPTALAGVTVFLDRDGTLNVDTGYVKTPEEFTLLPGVGAALARLKGG
                     GAKLVVVTNQSGVGRGYFSSKDLEAIHNKLRLLLAEDGVTLDGLYFCPHHPDDRCNCR
                     KPSRGMVDRALAELQVDLSRAYVVGDSVRDVELAKQVGARAVLVMTGPSGAEALADLT
                     ARDLAPDHVAADLSHAVEWTIAHATRKPVADVTG"
     assembly_gap    52268..52367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<52368..52705)
                     /locus_tag="PK589_00845"
     CDS             complement(<52368..52705)
                     /locus_tag="PK589_00845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNJ18165.1"
                     /translation="MRKEEIRRIVVRGPNWLGDAVMCEPALSQVRTLFPQAEITLLVK
                     PAIADLLAQHPAVNRTLVYDDRGRHAGLTGKWTLAGVLRRHRFDLAILFQNAFEAALI
                     SFLAGIPRRFG"
     gene            complement(52698..53756)
                     /gene="lpxK"
                     /locus_tag="PK589_00850"
     CDS             complement(52698..53756)
                     /gene="lpxK"
                     /locus_tag="PK589_00850"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNJ18166.1"
                     /translation="MRQSWLRWVGYPYEVAARLRLWGYARGWFQTHRLPRPVISIGNL
                     TMGGTGKTPVVMYVVDRLIAQGKRVAILSRGYRRRSKAPQLLVSDGQQVLVGPEEAGD
                     EPYLMARRCPHAVVAVGADRYALGQWVLSRLPVDCFVLDDGFQHVQLHRDVDLLLVDA
                     TDTAGLRAALPVGRLREPLSAAARASAVLITRVDREEAGEQVWRQLVQACPALPSPVC
                     VGFPAEEFRRIGGDEGRPRDAFRGRSAVIFSGIGNAGSFHALVAELGITVIETLAFPD
                     HVHYTHAMMETIRARAQAGGVDLLVTTEKDADKVAPFLNADDACWAIRLRTQIISGQE
                     RLERLLRLEPESRPASDA"
     gene            complement(53762..55117)
                     /locus_tag="PK589_00855"
     CDS             complement(53762..55117)
                     /locus_tag="PK589_00855"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HNJ18167.1"
                     /translation="MWYLLYNSLLLLVSPVILCVLLAKQRCRRGLPQRLGLLPEQPRE
                     AAPAPTCIWIHAVSLGEVVAVAPLVRELRRRYPEARLVVSTVTETGREAVEQRLEGLA
                     EHRYAPLDFPWVVNQTIDLLRPNLYVFVETELWPNLLRSLRRRNIPSILVNGRLSTRS
                     FERQRLPLIRDFYRTMLNMISCCLMQSERDAQRMVELGADQSRVRCTGNIKFDQPVPQ
                     AAAGGTVVSKQALGLSERERLVVAGSTHPGEEDMIVDAYQSVCEEFPELRLVLAPRHI
                     ERAAQVEQMVRAKGLPVSRRSTGKMELMATSGPRVVVLDTRGELALLYREAEVAFVGG
                     TLVPIGGHNLLEPAVWGKPVLFGPHTDHCAEVATLLLNARGGRVVDGAQGLAQSLREL
                     LGNSDDLKRMGQAAQQVVADNQGALQRSAEIIATFLPPSATSTRSEGATRGALPHPRS
                     S"
     gene            complement(55167..55829)
                     /locus_tag="PK589_00860"
     CDS             complement(55167..55829)
                     /locus_tag="PK589_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNJ18168.1"
                     /translation="MTNRSPAAPQPKQALTKRLTNALKLAVVPPVGYAVIRLLQRTMG
                     WRTEGREHVSRIFAQGQQAILAFWHAQQLMMPLAIPGLKAHILISQHRDGELICRIVA
                     RFGLDAVRGSSTRGGAEAFRRLVRLGRSGGNLVLTPDGPKGPRQVAKVGVVQLARATG
                     LPIVPMAFGCSKKNSSRAGTGSSCPIHSRRGSSSSGHRLQSRRMHPLKTWSACAGSWK
                     RP"
     gene            complement(55864..57600)
                     /gene="msbA"
                     /locus_tag="PK589_00865"
     CDS             complement(55864..57600)
                     /gene="msbA"
                     /locus_tag="PK589_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HNJ18169.1"
                     /translation="MKHYLRLLAYLNPYRFRLGAAFVCALLVAGLSAAYAWLVRPVLD
                     GLFISKDESLLLVLPVAILLVAVLKGVFNYGQNYLMNYVGNQVIGDIREQLFSKLVRL
                     PVHFHDTNTSGRLVSRVINDVNQMANAVAGVLKDLFQQGLTFLAMIGVIIYQNWKLAM
                     VSMIVVPLSVVTMARMGKKLRGLATRGQERMGDMASTLQEALAGIRMVKSFGREEDEA
                     KRFRQSNDAFIHTTMKAIQVSSLGSSHMEVIGVLGVAGIIWYGGYLVIHDEMTPGAFF
                     SFLAAMFMAYTPIRRLSGANNTVQQALAAAERVFDVLDLPTEQVANGGQTELPQISRS
                     LEFRQVAFQYEGQGDAALKAIDLTIRAGEVIALVGSSGSGKTTLVSLLPRFYDPSGGQ
                     ILIDGVDIRNCTLQSVRGQIGIVSQEVVLFDDTVRNNIGYGRQGATIDDVMQAAKLAY
                     AHDFISRMPDGYETLIGERGLKLSGGERQRLAIARAILRDPPILVLDEATSALDTQSE
                     RIVQMALSNLMRNRTTLVVAHRLSTIQNADRIVVLDRGTVAEIGTHDELLRKGGLYKR
                     LHAMQFADALPQ"
     gene            complement(57597..58730)
                     /gene="lpxB"
                     /locus_tag="PK589_00870"
     CDS             complement(57597..58730)
                     /gene="lpxB"
                     /locus_tag="PK589_00870"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNJ18170.1"
                     /translation="MPRILIVTGEASGDLHGAHLVKALKELAPSLQIVGIGGASMRAA
                     GAELVKDIPQLDVMGLIGLSAVKAMLQRISRIRALIKRERWDLVVLIDNPGLNFHFAR
                     VARACRLKVVYYIAPQVWAWRRGRMRWIQQRVDHVLAILPFEEPLYKKAGVRCTFVGN
                     PILDEVASTYDREALRRQFELSDAGPVIGLFPGSRKAELLEHVPLLIETVKRLAQRHP
                     TMQFILAQASSVQDQFLADLLKDSPVPIKVLRNQASEVMAASDLLVVKSGTSTLQAAV
                     VGTPMILFYRAPSWVTYRLARLLIRVPWIGLANLVADRGIVPELIHDEATPERLAQET
                     ERLLADPRAYAEMKAALLSVRQALGTPGASRRAAEAVLAECRS"
     gene            complement(58770..59759)
                     /locus_tag="PK589_00875"
     CDS             complement(58770..59759)
                     /locus_tag="PK589_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNJ18171.1"
                     /translation="MTALRAGVIGVGHLGQHHARHYATLPGVTLAGVYDASPDRAKLI
                     AERHGVQAWTNLGEVLTQVDLVSVAAPTSAHFAAAKACLEAGKHVLVEKPIAVTSAEA
                     HELVELAARRGCLLQVGHSERFNPIMQRMRASIERPAFIEGHRLSAFGERGTDVDVVL
                     DLMIHDLDLVLSFQPGPVEEVRAAGVPVLSSNIDIANARIAFASGCVANLTASRVSTN
                     KMRRLRFFQRDRYVSIDFQSRQAMVSRRVQGTGPRPTIDVETFQAGDEEPLRLELESF
                     IHAVATGSRPVVSGEDGEAALNLAGRVLDAIGQFTQRHSAGDVSAAAFGHDSL"
     gene            complement(59812..60654)
                     /gene="lpxI"
                     /locus_tag="PK589_00880"
     CDS             complement(59812..60654)
                     /gene="lpxI"
                     /locus_tag="PK589_00880"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249556.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HNJ18172.1"
                     /translation="MGGRAAHDSQAPEGQRIGLIAGNGRFPIIFADNAKRLGYSVFAV
                     AHEGETDPELAQHVDNIHWIKIGQFGKLIDALKGDGVQQAVMLGGIKKTHVFTTVRPD
                     FRALALAAKLIHLKDDDILRRVAEEIEQEGIRICESTFGLQGILVEAGTLTRREPSKK
                     EWEDIRYGWDVGKQIGALDIGQCVVVKDRVIVAVEAVEGTDGAIRRGGELAQGGAVVV
                     KRCKPQQDLRFDLPAVGPRTIEVMEAVKASVLALEAGRGILLDRDDTIAKANRAGIAI
                     VGMT"
     gene            complement(60660..61469)
                     /gene="lpxA"
                     /locus_tag="PK589_00885"
     CDS             complement(60660..61469)
                     /gene="lpxA"
                     /locus_tag="PK589_00885"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNJ18173.1"
                     /translation="MKIHPTAVIHPKAVLADDVEVGAYSVVGEHVTIGAGTRVLSHVS
                     IDGWTDIGERCELHPFVSVGGPPQHMQYKGEPTKVVIGHDNILREYVTVNRATVQGGG
                     VTTVGHSNFLMAYVHVAHDCHLGNHLILANAASLAGHISIGDHAIIGGLSGIHQFVRI
                     GAYAMVGGCCALGQDLPPFMRAAGGYRARMYGLNSIGLRRHGFSAERIAALKKAYEVL
                     FRSGHRVAEAVKLAREQFGASQDVLQVADFMQGTKRGICRSVGKDQEGEEE"
     gene            complement(61596..62060)
                     /gene="fabZ"
                     /locus_tag="PK589_00890"
     CDS             complement(61596..62060)
                     /gene="fabZ"
                     /locus_tag="PK589_00890"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNJ18174.1"
                     /translation="MSVMDNVEIQSILPHRYPFLLVDRIRELDPDRRIVGIKNVTINE
                     PFFQGHFPGRPVMPGVLILEALAQVGGVLAFKSLGAVGRPVVYLTGIDGAKFRKPVVP
                     GDILRLEVDVLKKRAPFWKMQGKAFVDTELVCEAEVTAMVMDEKAAGEKAAG"
     gene            complement(62103..62639)
                     /locus_tag="PK589_00895"
     CDS             complement(62103..62639)
                     /locus_tag="PK589_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNJ18175.1"
                     /translation="MNVRNWMAAGVLQVLVAWGAQPVLAADHLKVAMMDQQQVIERSV
                     AGKRALEELKSYSTTRQKIIDSDDQELKDLEKGMQDASMSEDARKEKQEHFRTKLEAY
                     QRRIQDFNREVQEKQRGMVAEYAQKIQAAAAAVAQKEGYTAVLDKGSESTVKIVIYSH
                     PSVDLTEQIIKEFDRQNK"
     gene            complement(62650..63225)
                     /locus_tag="PK589_00900"
     CDS             complement(62650..63225)
                     /locus_tag="PK589_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNJ18176.1"
                     /translation="MRGLVGWKSVRGAGWNGLCLALLAVTLLVGGCRSNPSTVPTSTR
                     IGVVDPQRILGETNAGKRAKDMLASFAKNRQALIELEEKELRRMEEDFVKQGSVLSAT
                     AKREREEQFRRRMAEYQQKVTDLNREVQDKQKEVLDGFRDKIESLSAKVAKRLELQAI
                     FDRGRGGPTLYFDEAVDVSSQLIEEFNKTYP"
     gene            complement(63267..65591)
                     /gene="bamA"
                     /locus_tag="PK589_00905"
     CDS             complement(63267..65591)
                     /gene="bamA"
                     /locus_tag="PK589_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNJ18177.1"
                     /translation="MTKSGGRRWVPILVLVLAALFVIGEAGWVRAQVSAPLVNSVTIR
                     GQKRIELQAIQGRLTLKANDRFTPDALREQVKILYGTGYFEDVQVETETADGGVAVGF
                     IVREKPFITEIVFDGNEELSDDKLKEKITIKSQTFLDQQQAKESAEKIRLAYQEDGFY
                     NCQVIPVIQAVDEDRKRLTYFIKEGTKAKVRRINFEGMRAVTKEEVFKVTATREWIPW
                     YGLVTQLKLPSLLSDAGVLKREELGNDIERIREVYLNKGYLNVQISQPTLELSDDKKW
                     FEISYSVVEGEPFIVQEVGFRGNTVFEDHELREGLGIRPGEIFQRAKIRGEITRITDL
                     YGAKGYVFADVVPNVTPDNNARTATITLNVKEGEMMRIREIHVTGNDKTRDNVVRREL
                     RLDEQDVIDTLALKRSFQRLNNLNFFETVEILPQQVDVDKVDLNVKVKEKPTGQFSIG
                     GGFSTLDKLVAIADITEGNLGGNGWLGRIRGQLGQQRSLGLVTFRNPYVNDSYNALQL
                     DIYRSMTNYISYFESKSGASATWSRWLSEYVNGSISLFGEQLKYSDPQEGLCPDLIPL
                     ICRQLGTQSSTGFRTSLFRDTRDYYLDPRTGWRLSVGADYGTPALGGSNHFYKVYFDV
                     IKYTPLVYDTRFSVHVRYGQTEGIGGRPIPLTERFFVGGINTMRGFVFGRAGPVTPSG
                     SLLGSAKELIFNNDFIFTISSEAKLNGVFFFDYGNGFDDNEPVQFNKLRSAAGFEARW
                     ISPFGPLRAAYGINLDPRPTERMGVFEFTIGSLF"
     gene            65907..66833
                     /gene="galU"
                     /locus_tag="PK589_00910"
     CDS             65907..66833
                     /gene="galU"
                     /locus_tag="PK589_00910"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNJ18178.1"
                     /translation="MTRTDVRKAIIPAAGLGTRFLPATKASPKEMLPLVDKPLIQYVV
                     EEAVASGIEDIIVITGRGKRAIEDHFDRSLELEENLKGSGKGQVLNQMRHISNLANFC
                     YVRQPEAMGLGHAVLCAQHLIGDEPFAVILGDEIIDAAVPGLAQLIHIYKQRKGAVLG
                     VQEVPQQEVSRYGIISARKVRSGLHRVEDLVEKPAPADAPSNLAVIGRYVLPPEIFPI
                     LRKTPPGKNGEIQLTDALRQLVKNTPMFAHEVEGQRHDAGDKLGFLIATVELALKNPS
                     LGPAFGDFLQQRLRPTTADVRRRGPGRGRATT"
     gene            66920..69418
                     /gene="lon"
                     /locus_tag="PK589_00915"
     CDS             66920..69418
                     /gene="lon"
                     /locus_tag="PK589_00915"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNJ18179.1"
                     /translation="MAEAVEQDFSSNQNLEPPDQLPLLPVRDIVVFPYMVLPLFVGRE
                     MSIKAIEAALAGNRMLFLATQKSLDVENPQPEDIHSVGTVGIIMRMLKLPDERIKILV
                     QGLAKGRIEQYIQNDPYYSVKIEKLVETKQPGSTLETEAVMRTVKEQIEKIVSLGKVL
                     IPDVMVVIENLEDPGRLADMVASNLGLKVDITQAVLEIVDPIQRLRQISEILAKEIDV
                     LSMQQKIQAQAKGEMDKTQREYFLREQLKAIQKELGELDERAEEVAEFRKRIKDAKMP
                     EKVLKETEKQLKRLEKMHPDTAESATVRTYLEWMVELPWNKKSKDNLDLKAAMKVLNE
                     DHYDLEKVKERIVEYLAVRKLKEKMKGPILCFVGPPGVGKTSLGKSIARALGREFVRI
                     SLGGVRDEAEIRGHRRTYVGALPGRIIQGMKQAGTNNPVFMLDEVDKVGMDFRGDPSA
                     ALLEVLDPEQNSTFTDHYLGVPFDLTEVMFVTTANLMDPILPALRDRMEVIDIPGYTE
                     EEKLGIAQKYLIPRQMNEHGITEKHIRVSETAIRHVISHYTREAGVRNLEREVANLMR
                     KVAKKVAEGKAECHAIDQTNLNKYLGVAKFIPEAELEKDEIGVATGLAWTESGGDVLY
                     IEATVMKGKGQLTLTGHLGDVMKESAQAALSYVRSREKTLGISPDLFAKNDIHIHVPA
                     GATPKDGPSAGITMATAIASALAQIPVRRDLAMTGEITLRGRVLPIGGLKEKILAAKR
                     AKLSTVALPKRNKKDLEEIPKHILKGIELIFVDTVDDVIKAALRRAPSRKATAGAPKT
                     PLKKPSPVTASKRKAAASPRRRRALTGTTRSLSL"
     gene            69494..70534
                     /gene="thiL"
                     /locus_tag="PK589_00920"
     CDS             69494..70534
                     /gene="thiL"
                     /locus_tag="PK589_00920"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNJ18180.1"
                     /translation="MPPGRTRRASTVQSEFGLIRKLRAQAAQPDPQVFKGIGDDTAIL
                     KTSSTEWTLITTDLLAEGVHFDPTTSSFRDIGYRAAIANLSDIAAMGGTPRFMLVSIA
                     IPRACSTTQIQQLYRGMTEADAPYRVRLVGGDTSASKTGLFLNITLTGVVKPRQALLR
                     SSARIGDLIYVTGTLGDSRAGLDLLTSRRRPKLRPTDARFLLGRHHRPSARIAEGLWL
                     VRHGLAGAAIDLSDGLTGDLRHICEESGVGAEIIAGSLPLSRACRAYAMARGLDPVEI
                     ALQGGEDYELLFTVPRGQQLRFERLVKKTGFRMTCIGSITPKAAGLRLRTDAGATRPL
                     PLTSYEHFRRPT"
     gene            70557..71036
                     /locus_tag="PK589_00925"
     CDS             70557..71036
                     /locus_tag="PK589_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNJ18181.1"
                     /translation="MADVRSLFRQILHLDETPHRTALAFAVGVFIGFSPAYGLHMIMV
                     GLCAWLFGWNVVALLAGAFLNNPWTLIPILGATYWTGATLLGMREFPTFDWSDLSFTG
                     LYHQVAPHAWPFVLGGMVLSIAGSLLAYPIAYVLLTRYRRARAVERATHLPPPSGLG"
     gene            71060..72481
                     /locus_tag="PK589_00930"
     CDS             71060..72481
                     /locus_tag="PK589_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNJ18182.1"
                     /translation="MKLPSSTPPAPYDGSALIADPIHEYVSFTVPYATPDPSERTEKD
                     LIDSPWVQRLRYIYQLQSARWVYPSAEHTRFVHSLGTMHVAGRFARHLYPFLRNAVPD
                     LPSAAFVEEFLRITALVHDIGHGPFCHFFDDNYLHGFGLSHEKLGQIIVREHLGPLIK
                     KIRRSPSGPFDRGEELNPDHIAHVILKEKGKDNSKIPRWINVLQPVISGSYTADNLDY
                     VLRDAYMCGVAVGPVDLTRLIHYTIVTEKGFTIHKTGLPALHMFLNTRMYLYSNVYYH
                     RTTRAIDIHLRDIFGDTMKLLFPSNPAKHMDEYLTLTDWSLLEDVRRWKKAGQSSLRR
                     LHQEWAHILSRDVKWKMAYSTVLKEKGIERGMDFPSHEQFQQQIQNALPAKLRDLPFR
                     VDMAPLDPRPDPKDTRGIPLLVYDPGTKGLSTEPLEEFLDLLPTRLVQFRIYALDHAH
                     DAALSRAAATVLNKTPSSIETNF"
     gene            72680..75433
                     /locus_tag="PK589_00935"
     CDS             72680..75433
                     /locus_tag="PK589_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ18183.1"
                     /translation="MTSNGLYGLMTDKLTPRIRELGIGAFIAALCGAMFVLDCLTPRG
                     IPGWVLYTLPIVLTTWTPHPWSSHAVAVGCAGLTYLAYVLSADVPGVPTWLPQVSRSF
                     SIVLFPLIAYVVDRQKRLTSELLKSTTMAAEHKALLDRSQLLRKTAEEVQDLYDRAPC
                     GYHSLDANGLIVAINRTEVDWLGYTKSELIGKIRFTDVMTPAGAGLFKERFPQFIKDG
                     YVNDLEFELIRKNGSTFSVLLSATAVTDSDGRFVSSRSTLIDITERKRADQALRLSHQ
                     SLEALVEERTAALQEANSRLEQELAQSKQMHQALIASETRFRELVESLPLPIWTCLPD
                     GTCDYLSPRWANYTGRSADEPFSTGCLQHVHPEDRPRITEQWHHSVAHIQPLDTELRF
                     KRADGIYRWFHAHATPIRDHDGHLLKWVGTYTDIHDRKQAQEIQARLASIVESSSDAV
                     LSKGLDGRILSWNKSAELMFGYPSEEIIGQPVSRILPPERRAEEAILIEQITAGIRIQ
                     QFESVRLHKDGHPIQVSLTVSPIVDANGEVTAVSTIARDITERKRAEEVLSQQRRLIE
                     LSYDPIFTWDFERGILDWNRGCEQLYGYTRAEAIGRSSHTLLKSTYPCPFDTICAELE
                     ATGAWTGELHHRTKDGREVLVESRWGLMKTGGRSLVLETNRDITERRQAELMILKKNK
                     DLETLLYVTSHDLKEPLRAIESFSVLLQERYASRLDEKGQDFLRRIVRATQRLDQLLT
                     DILDLSRAQRMEPPVDEIDAHQLVQEVLRRLESRIKESGATITVCTPLPRLRVNTTWA
                     VQGIYNLVANALKFVADGRPPDIEIAPHTEIDAEGTECSGLVIRDRGPGVPSDQRERI
                     FELFRRAVGREIEGTGAGLAIVRQVAERHGGRAWVLPREGGGSDFFITFGINRQVVKE
                     VGL"
     gene            75430..75870
                     /locus_tag="PK589_00940"
     CDS             75430..75870
                     /locus_tag="PK589_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ18184.1"
                     /translation="MTMRPFDILIAEDNEDDILLIREAFETVNMTNRVAVVRDGAEAM
                     AYLRGEGSFSQCPLPGMVLLDINMPKKTGLEVLEEIKSDPRFRAIPVVILTVSEREED
                     IVRSFEKGACSYIRKPVTFTRFIAVVKEFELYWTLVSKVPTLKR"
     gene            75874..78330
                     /locus_tag="PK589_00945"
     CDS             75874..78330
                     /locus_tag="PK589_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ18185.1"
                     /translation="MQVLLIEDNEDDAALIREALCEHSPQDVLLTWADRLEAGLLKLA
                     EGSPDAVLVDLSLPDSQGMDTLVRVRAQAHEAAVIVLTGLDNDLVAEKSLLHGAQDYL
                     VKGRLTGDGLKRAIRYAMGRHRVEQALRKSEERFHLTCLATRDGIWDWDITSGETWFN
                     DAYQTVYGSGAKAREAGRIAWADQIHPEDRQSILAGLSHTLESDKNLWTAEYRFRRAD
                     GTYAYVIDHGYVLRNYAGTPYRMIGAKTDISDRRQAETMHAVQLTVGLALEESVSLNE
                     AVPKILRAMCELQGWTLGALWLVDTQHSVLRCNALWHPSGQQTEHFAQAYRTLFLRQD
                     MGMAGRAWKTDAPAFSPDIVNEADFPAHDAAREADLHGAIAFPIHTGKEILGIMEFFT
                     VEVLHPAVSRMERIAELGAMISQFLNRKDLERQLRQAQKMEALGRMAGGVAHDFNNLL
                     TIINSWAELLMDEPGLTSRAQRGMAQIREAGNKATGLTRQLLAFTRHQVVERQSLNLN
                     ERVSDIVELMKRVIGEDIHLVLTLDPTLGRIKADPGQIEQVVMNLVVNARDAMPHGGR
                     LELETGEVMISHSDPFWPDPLPPGPYVTLSVRDTGCGMSAETLAHVFEPFFTTKELGK
                     GTGLGLSTVYGIVRQSSGTVGITSEVGKGTSFTIYLPRLEEDTGAPRAVTQPKVTSEQ
                     SETILLVEDNDMVRGLAHTVLVAQHYHVLAARSGEEALEMVRRQGRHIALLVTDMVMP
                     GMGGAQLAHELQTLQPDIKVILTSGYSGRDETLLKTFDAQVAFLPKPYTPESLTKAVA
                     SALESQTRQGSCRDTSSHST"
     gene            complement(78380..79363)
                     /locus_tag="PK589_00950"
     CDS             complement(78380..79363)
                     /locus_tag="PK589_00950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNJ18186.1"
                     /translation="MSFLDRFLIISVVLLASVFPVELFPNTGPVPRGGDGQFSGKQGQ
                     VVVVTVPELKEATSVKGKFLGRTVTLFPNPTAGGTGYIGLLGIDLQDEPGPHELTIDA
                     QLGEQTRHFSYQVLVVKEKFAVEHLKLPKDKVDLDEKATARWKAEQEQVRKALAEESA
                     MRLWQAGFIEPVHGKRTGIFGSVRIMNGQPRNPHNGEDIGAPMGTDVMASNDGVVRLV
                     VDHIFSGRGIFVDHGLGLYSMYFHLSEALVNEGDLVKAGQVIGKVGATGRATGPHLHW
                     GMKVNGARVNPYALLELPFPQNPAADRPVLAAPAVPAQEELAPTAVGGDGR"
     gene            complement(79396..80772)
                     /gene="pafA"
                     /locus_tag="PK589_00955"
     CDS             complement(79396..80772)
                     /gene="pafA"
                     /locus_tag="PK589_00955"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HNJ18187.1"
                     /translation="MQQRIFGLENEYGLIFSPNGKIYLPMEKVLGYIFEGLIPNSWPS
                     NAFLVNGARFYQDTGCHPEYSTPECDNILDLVIHDKAGERLLEACLPAAEERLREEGL
                     SGEIYIFKNNTDSLGNTYGCHENYLMRRDVDFWKVTEQLIPFFVTRQIYSGAGKVLKV
                     SGKPQYFISQRAQHIHEKTSSSTTSSRSIINTRDEPHADAEKYRRLHIIVGDSNMSEY
                     ATYLKVGTATLVLSMIESGFSVTGMELEDPVKAIREISRDPTLNKKVKLDDGRQMTAI
                     EIQRVYVKRATEFLAAQDHDPVLDDVLDKWISVLDRLEDDPMQLVHEVDWVMKKHLIQ
                     SYTDKKDCGWDDPRVFLLDLQFHDVKRTRGLYYLMENRGMAARVVEEEAVQRAMSVPP
                     QTTRAKVRGDFIRFARAKNRSYTVDWTYLKLNGYWEETILCMDPFSAVNRRVEELLSQ
                     VSGARLYR"
     gene            complement(80926..81609)
                     /gene="prcA"
                     /locus_tag="PK589_00960"
     CDS             complement(80926..81609)
                     /gene="prcA"
                     /locus_tag="PK589_00960"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HNJ18188.1"
                     /translation="MPLPYYVSPEQMMQDKAEYAKKGIAKGRSIIALEYVDGIVLAAD
                     NPSSSLHKVSEVYDRIAFAGAGKYSEFEHLRKAGIRHADLTGYMYSREDVSARTLSNA
                     YSQSLGTAFSTDVKPLEVEILVVQVGGNGQANEIFRISFDGSIVDEKSFAVIGGRSEA
                     VQQYLREHATPQPPTLKDGLRRCLSALEQVATQKIPSENLEVAVLDRNRQGRKFRRLT
                     SADISHLLA"
     gene            complement(81613..82437)
                     /gene="prcB"
                     /locus_tag="PK589_00965"
     CDS             complement(81613..82437)
                     /gene="prcB"
                     /locus_tag="PK589_00965"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HNJ18189.1"
                     /translation="MNRMPFLPHHDGSSFFDFLTQYHPELRPGVHDLAMPRATLPLDL
                     GRPGAIPVPHGTTVLALKYRDGAIIAGDRRATEGFQIADRRIEKVFRIDEYSAMAIAG
                     AAGPCIEMAKLFQTELEHYEKLEGVQLSCEGKANKLGQMVKANLPMVFQGLVVMPLYV
                     GYDVKRKEGRIFKYDITGGRYEESDHHSIGSGGKDARNTMREHYRSGLDEEAALRVGL
                     LALYNAADEDVGTGGPDLVRGIYPTAKVVSDAGITDVPESRVRALYEAMIAERRRS"
     gene            complement(82451..83950)
                     /gene="dop"
                     /locus_tag="PK589_00970"
     CDS             complement(82451..83950)
                     /gene="dop"
                     /locus_tag="PK589_00970"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HNJ18190.1"
                     /translation="MNDTHSHNLSRVIGTETEFGIASRDPNAADPVANSIHVIGYYPN
                     LPAPQAVWDYENENPLLDARGFEVDGERERPGPDYNRQLNKVLANGGRLYVDGAHPEY
                     STPECTNAREVVAFERVGERIIAQALAGITEARGRDQFVLYKNNSDGKGNSYGYHEDY
                     LVSRAVSFDRITQVLTPFLVTRPIYAGSGKVGAENQTPSVDYQISQRADFFETLVDLN
                     TMVRRPIINTRDEPHSDSTKYRRLHVIVGDANMAELSTYLKVGALSIVLDLLEAGADL
                     PRISLADPVNAIKQVSRDTQVKESLKLTDGSATTAIAVQRAYLKAAQSYYACHELNQV
                     TKDILVRWEDVLDRLERDPRLLVRELDWVAKRYLIESYMDRKSCGWDDPRVRLMDLQY
                     HDVRPDRGLYYTLERSHMIERVVLEHEIVRAETNPPVGTRAYFRGQCVKKYPNAVYGV
                     SWTSVLFDVGQNKIKRIPLMDPLRGTEALTGELFAHAETAAALLEKLST"
     gene            complement(83965..85641)
                     /gene="arc"
                     /locus_tag="PK589_00975"
     CDS             complement(83965..85641)
                     /gene="arc"
                     /locus_tag="PK589_00975"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HNJ18191.1"
                     /translation="MTHKNDEHAREVEKLRTQIQSMEEEIRRLYQSRYQLEQTTKQNE
                     KLVATLQEAKSQIETLRAEVEKLTAPPSTYGIFSSLDTDGTGNVYVSGRKMKVSLHPS
                     IKAKELRKGQEVILNEALNVIAVRGFDVQGEVVRLKDVLEGNRALVTLHFDEEKVAEL
                     GEPLLSERLSVGDHLLYDPRSGCVIEKLPKSEAEELVLEEVPDVDYEHIGGLQREIEQ
                     VRDAVELPFLYPHIFSNYKLSAPKGVLLYGPPGCGKTLIAKAVASSIAKKLGHLKDRQ
                     LRSYFLHVKGPELLNKYVGESERQVREVFKKAKERADDGHPVIVFFDEMDALFRTRGT
                     GISSDIESTIVPQFLSEIDGVERLTNVIVIGASNRQDLIDPAVLRAGRLDVKVKVGRP
                     DATAARDIFSKYVSTDLPFAEEDLARHGGDARALVDSLMNLTVDAMYATTDENKFIEV
                     TYANGEKEVLYFKDFASGALIEGIVSRAKKFAVKRAIAQEGSGLRADDLIRAIREEFK
                     EHEDLPNTTNPDDWAKVAGKKGEKIVHLRTISGGPSEARQIETVSTGHYL"
     gene            complement(85891..86517)
                     /gene="thiE"
                     /locus_tag="PK589_00980"
     CDS             complement(85891..86517)
                     /gene="thiE"
                     /locus_tag="PK589_00980"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNJ18192.1"
                     /translation="MSAVDFRLYLVTDRHQTAGRPLGSVLARAVRAGVRAIQLRERDL
                     PVRDLHALATELQRELPDARLFINDRVDVALALRTVGVHLRESSLPAGVARCLLGPSQ
                     LLGTSVHSLDGVVAAEQQGADFVVLGPIYDTPSKRVYGPPLGMRILEQAAQKVRIPIF
                     AIGGITAARTREVRQAGAFGVAVLSSILSAVNVEQATEELLAAIQMES"
     gene            complement(86627..87397)
                     /locus_tag="PK589_00985"
     CDS             complement(86627..87397)
                     /locus_tag="PK589_00985"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNJ18193.1"
                     /translation="MNDRLVIAGREFQSRLWVGTGKYKDFAETKKAIDASGADVVTVA
                     VRRVNITDRSKENLLDYLDPKKYIILPNTAGCYTVEDAVRYARLARAAGVSDLVKLEV
                     IGDEKTLFPDTAGLIEAAKILIKEGFIVLPYTNDDPIVAKKLVDIGCPAVMPLAAPIG
                     SGLGIRNPYNLKIIMETVKVPIIVDAGVGTASDAALAMEYGADAVLMNTAIAGAQDPI
                     AMAEAMKYGVWAGRLAYKAGRIPRKLYATASSPIEGML"
     gene            complement(87409..87615)
                     /gene="thiS"
                     /locus_tag="PK589_00990"
     CDS             complement(87409..87615)
                     /gene="thiS"
                     /locus_tag="PK589_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNJ18194.1"
                     /translation="MTIQVNGESRGIGDGHTVAALLRDLDIRADRVAVELNLEILDRK
                     DFETRGLCEGDRVEILSFIGGGAE"
     gene            87747..88238
                     /locus_tag="PK589_00995"
     CDS             87747..88238
                     /locus_tag="PK589_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HNJ18195.1"
                     /translation="MDDRLNQLAQIFRAMANLLAAQRANPYRVRAYRKAADAILMITG
                     DLTDLAARRQLQEIPGIGKDLAGKIEEFLTTGTIRAYEELRTPLPKEVAEWASLPGLS
                     DALVSYLYFRLNIRSLADLESLVASHLLRTQPGFSGSEDDLLAAIRRQRASDAVTPPV
                     EAP"
     gene            complement(88213..89559)
                     /locus_tag="PK589_01000"
     CDS             complement(88213..89559)
                     /locus_tag="PK589_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNJ18196.1"
                     /translation="MEQRRSRRWLYLLLMVTVALGVGIVLEKGLERTGHASETYEELR
                     TFSEVLTQVQKHYVDDTKVKDLVQGAIRGMLSTLDPHSAYMTPEMYKEMQVETKGEFG
                     GVGIQIGVKENRLAVISPIEGTPAHRAGIKAGDFITKVNDEPTKDLTLMDAVQKMRGP
                     KGTKVNLTIQRDGTADPLAFSLVRDTIKIESVKFKVLDNTIGYVRLTQFQEATGRDLA
                     RALKQFKEQKVQGTILDLRNNPGGLLTAAVDVSEQFVGNGKLIVYTKGREGKKDEWFS
                     KTKETLEDSPMIILVNEGSASASEIVAGALQDWGRAVIVGTTSFGKGSVQTILPLGDG
                     SGLRLTTAKYYTPKGRSIQSTGITPDIVVKLQTQTVAKAGDKDAKEAEPKTAKAPASG
                     KDSASSKPADEPAHKNGAASLNDAGGDISVEDDVQLQKAVELLKTWKIFKELRPAA"
     gene            complement(89584..90780)
                     /locus_tag="PK589_01005"
     CDS             complement(89584..90780)
                     /locus_tag="PK589_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNJ18197.1"
                     /translation="MTRIQWVWVVLSVHLALSTSPPPAHAERKDSYADKIEQEKKTLE
                     KLRGTIVEKRKKADEAEKKRESILQGLQSLDERLVRYRQEHQDTVRKLKKKDVEIEQM
                     SVQLSRLSERIDERQDAIAARLRVQYVEGRFWQLKTLLAASSSGDFQRRFRYLSAVTQ
                     REYEIVEGYRKDAERIAEVERSREEARQGILLYKISTEEKLAQIQGLKKQKRVYLAKI
                     TQEKESHDRAVEELERSASRVDSLLKELETRRRAALANRPPSAGAGLRALRGTLLWPT
                     DGQVVSYFGRQKHPTFNTYVQRKGIEIRAAEGSIIRSVLAGQVVYADWLKGYGLVIIM
                     DHANGVFSLYAHASKILTSVGARIEAGNAIGETGDTGMTGENTLYFELREGAEPVDPL
                     VWLSKR"
     gene            complement(90777..91676)
                     /locus_tag="PK589_01010"
     CDS             complement(90777..91676)
                     /locus_tag="PK589_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNJ18198.1"
                     /translation="MRRLLYLLREAVANVLTNRTTTLVAVATTAFTFACVGVFLLLYV
                     NLRTMASSLEQDIQVMVYLQDDLTEQARSEIEQQLKIDRAIASLTFVSKERALADFQA
                     QFPSESRLLQGLGQNPLPASFVVLMAPESRSSDAMRRWANRTQLIPGVSQVQYNQEWV
                     EALAGIVRYIEVAAIIVGVILSAASVTIIANTIRLALYSRREEIEILGLIGASTTFIR
                     VPYLLEGAALGLCGSALSLVILKGGFELFRHQIHSATRFLGVDALLTFFSFDMCLVLM
                     LVGLFLGCAGSFLSLLRFGEGRA"
     gene            complement(91673..92341)
                     /gene="ftsE"
                     /locus_tag="PK589_01015"
     CDS             complement(91673..92341)
                     /gene="ftsE"
                     /locus_tag="PK589_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNJ18199.1"
                     /translation="MIQLFHVSKYYDRRPALSDVTLEIEKGEFVLLMGPSGAGKSTLL
                     KLLIGAERPEEGQILVQGRSLSKLRPSEIPVLRRKVGIVLQDFRLLPKKTVFENVSLP
                     LLVQGASTTEIRRKVAEALRAVGLDHKKDLFPPGLSTGEQQRICIARAIVNGPIMLLA
                     DEPTGNLDPELTSEIIELFKSINARGTTIIVATHDPNVLAQVNRRVITLEHGKVVSRD
                     QGAS"
     gene            complement(92443..92829)
                     /locus_tag="PK589_01020"
     CDS             complement(92443..92829)
                     /locus_tag="PK589_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNJ18200.1"
                     /translation="MRDRRRLLGDEGEGQAEAYLRRQGYRILGRNVRSPLGELDLIAD
                     DAGTLVFVEVKRRRSGAYGGAIEAVDARKRAKLIQLASHYLAQHRLHDRACRFDVVLI
                     QDDAGSGAAVQHIANAFDVAGDDLRW"
     gene            complement(92829..93467)
                     /locus_tag="PK589_01025"
     CDS             complement(92829..93467)
                     /locus_tag="PK589_01025"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNJ18201.1"
                     /translation="MEARSRGYRHIAGLDEAGRGPLAGPVVAAAVLLPRRCRLPGLND
                     SKQIIEADRVRLFDEIVRRAIGVGVGAATEVEIDRLNILQASLLAMRRALMALPLQPD
                     FLLLDAVTLSGLSIPQRPIIKGDGLSCSIAAASIVAKVTRDRLMVEYHRWYPQYNFAE
                     HKGYATPEHLRLLREHGPCAIHRRSFAPVQHCFAEDDTVSLSLSDESESLFD"
     gene            complement(93557..93961)
                     /gene="rplS"
                     /locus_tag="PK589_01030"
     CDS             complement(93557..93961)
                     /gene="rplS"
                     /locus_tag="PK589_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNJ18202.1"
                     /translation="MNRLERIQRSLTKKTAPKFEIGDTVRVHVKVVEGEKERIQVYEG
                     TVIARKGTLNSETFTVRKLSYNVGVERTFPIHSPSVAKIDVVRQGRVRRAKLYYLRTK
                     KGKFAKVEDREFKVESKAQAAAKVEAAAAAKA"
     gene            complement(93977..94750)
                     /gene="trmD"
                     /locus_tag="PK589_01035"
     CDS             complement(93977..94750)
                     /gene="trmD"
                     /locus_tag="PK589_01035"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNJ18203.1"
                     /translation="MRCAVLTLFPDMVAPVLGQSILKRAQEKGLLEVSVQNLRDYTFD
                     RHKTADDVPYGGGAGMVMKAEPVLQAVDALRARYQASHPETALRVIMPSPQGRPFTQA
                     LAQSLAKDDRVLVFVCGHYEGMDERVNVALCPEQISVGDYVLTGGELAAMVMIDAAAR
                     LVPGVLGDAASAAEESFTEGLLEYPHYTRPAEVRGMAVPEVLVSGHHEVIRLWRRKEA
                     LRNTYLKRPDLLRDRELGLEDQRLLSEVMQESLVQVPVR"
     gene            complement(94752..95279)
                     /gene="rimM"
                     /locus_tag="PK589_01040"
     CDS             complement(94752..95279)
                     /gene="rimM"
                     /locus_tag="PK589_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNJ18204.1"
                     /translation="MTPLDQAELVTIGRIERSFGVRGEARVRSLSDVPGRFEALREVT
                     IVASSGKSLVTRVTHVRSGGPTLIVGFEAFTTPEQVAEFRGGLLQVPRGDSPALPSDQ
                     YYQCDLIGMVVQDEAGTVLGQLEEVLAMSDNQAFLVRQDGKELLIPAAKQIVLSVDVG
                     KRVMTVRLPEGFGDL"
     gene            complement(95289..95597)
                     /gene="rpsP"
                     /locus_tag="PK589_01045"
     CDS             complement(95289..95597)
                     /gene="rpsP"
                     /locus_tag="PK589_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNJ18205.1"
                     /translation="MAVHLRLARTGRHKRPMYRVIAADSRKPRDGRFLEILGIFDPLK
                     NPAVPVLKSERVLSWLRHGAQPTTTVRTLLKRHGVWKEFETEKSAKAEKPGKDEKSKK
                     "
     gene            complement(95698..97047)
                     /gene="ffh"
                     /locus_tag="PK589_01050"
     CDS             complement(95698..97047)
                     /gene="ffh"
                     /locus_tag="PK589_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNJ18206.1"
                     /translation="MLDALSEKFERILKKLRGQGVLTEENIAEALKEVRLALLEADVN
                     FKVVKDFLDRVREKAIGQEVLKSLTPGHQVVKVVWDELRGMMGGERSGISLASRPPTI
                     LMMVGLQGAGKTTTSGKLARLFKSQGKRVLLVAADPRRPAAGDQLASLGHDLGIDVHR
                     FDQVDASRADVVRICQRGVERAQEQGYDLVVLDTGGRLHVDDELMAELVAVKEAVTPH
                     EVLLVADAMTGQDAVNMAGQFDRQVGLTGVILTKVEGDARGGAVLSIRAVTGKPIKFL
                     GMGEKLDALEPFHPDRMASRILGMGDVLSLIEKAQETFSREEAEAAQKKLTSSTFTLE
                     DFRSQLGQMNRLGSFEQILGMLPGGQKLKDLANSGLPEKEMKRVAAMIDSMTSRERRD
                     HTLINGSRKKRIARGSGTSVQEVNRLIKQFLQARKIAKVMSGGAGGRRQLAQMLRGM"
BASE COUNT        21589 a        30218 c        25749 g        19480 t
ORIGIN      
        1 ggcccgcaga aacaaggcca gccccgcaag agcgctgaga agaacaaata tggcccgtgt
       61 taccatactg ttttcctcct ctctgtgatc agcctcgaat caacgactcg aagcaatcac
      121 ggcctcagcg tcagataaat ggcccgcccc tgtcgcttca acaggagcaa gaccgcctgg
      181 tcttttggca aatttgcggc cacatgttcg tacgacttga gcgtgcccac cgacttgcga
      241 ttcacctcca acacaaggtc gccttcgcgc acaccggcct cttccgcctg actgcccggc
      301 ttgacccgga ccaccacaat tccgcggtcg ctggacttga gcccatatcg gccggctaac
      361 tctgaattca cctcccgcac ttcgatatcc gcgagcagtc cggctggcgc aatggactcc
      421 ccgccttctt ctgcagcggc ctgcgacaga ttcttcggct gctcggcaat gaccaggtcg
      481 atggacttcg gcttcttctc acgaatgagc ttgaccgtga cttttttccc gaccacggtc
      541 tgagccacgg catttcgtaa gtgggccggc gaatccatcg gcttgccgtc atattcgaca
      601 atcacatctc cacgctcgaa cccggccttt ttcgccggac tatcatccat gatgtcgctg
      661 acgaggacgc ctttcacctc tttcggcacc ccgaactgcg aggacaattc aggcgtcaac
      721 tcttgaatgg aaacgcccaa ccagccgcgc acgactttgc catgctgaac cagctggtcc
      781 atgatggaat gggccatgtt gctcggcacc gcgaatccga ttcccatgtt tccgccgctt
      841 tgactgtaga tggcggtatt gatgccgacc aactctccgc gcacgttcac gagtgcgccc
      901 ccggaatttc ccggattgat ggccgcatcc gtctggatga agtcttcgta ttcggcgatt
      961 cctgccgcgc gccccagcgc gctcacgatg cccagggtga cggtctgagt cagaccgaaa
     1021 ggattgccca ctgccagcac gaattcgccg acttccagct tgtccgaatc ggcccatgcc
     1081 acggtcggca ggccggtcgc ctcgattttc aggacggcga catcggtctt cgcatccgtc
     1141 ccgacgagct tcgctttgaa ctcccgcttg tccgacaaaa acaccttgat ctcgtcggcc
     1201 ttattcacca catgattgtt cgtaatgatc aacccgttgg catcgacgat cacgccggaa
     1261 cctaacccct gctgtttccg ctccttcggc gcttcaaagc gcttcatcca ctcctcgccg
     1321 aagaagcgtc gaaaaaaggg atcctcgaac ggtgtaccct tgccttcttc gccgccccca
     1381 ttgcgagttg cgaaaatatt cacaaccgcg ggtttgacga tcttggcgac gtcgacgaag
     1441 ctctgccccc cggcgccggg cagcgtcggt tgcactgccg ttgaaaccgg cctgggaacc
     1501 ggagctggca cggcaggcgc ctccggcacg gcatgtcctg tcggtaacca tcccagatcg
     1561 gaggtcagga caatcccgat caggatgcct gctgataaca gcaatgctgg aaagatccag
     1621 gatcgcatcg actgtcgctg ttcaggtgga atcggaccgt catccatagt ggttcgtcag
     1681 tctggaccga ctagggcttc tcgcccgcgt acagactcat gatcgtgttc aaaaatttca
     1741 cggcttccgc cttcggccgc tggaatgaat tccggccgat gatcgacccg aatcctccgc
     1801 catcacggat cgcccgcact tcttcgaaga ccgttttttc gtcgctcttc gcaccgccgg
     1861 agaaaatgac gatgcgacgc ccgttgaacg aactctgcac gatatgcttt acgcgctccg
     1921 tcagcgtcgc cgtgggaatc ttttcggcct catacacctt cttcgccgct gcctgctcca
     1981 aatgggctga gggcaatttg accttgatga tatgcgcgcc gagttgcgcg gcaatctgcg
     2041 cggcataggc caccacgtcg agggccgtct caccttcctt gctcagccct gacccgcgcg
     2101 ggtacgacca gaccaccaca gccagcccgg catttttggc ttcctccgca atcgcgcgca
     2161 gctgctcata catgggattg cagtgagaag agccgggata aatcgtgaac cccacggctg
     2221 aacatcccag ccgcaaggcc tcctgcacgc tgcctgtcac cgagggcagc ggatccttct
     2281 catcgtgcag cacatcatga ttattcaatt tgagaatgag tggaattctg ccggcgaagt
     2341 ggctggcacc ggcctcgata aatccgagcg gcgccgcgta ggcgttgcat ccggcgtcga
     2401 tggccagttg aaagtggtaa tggggatcat agccgcccgg attgggcgca aaacttctcg
     2461 ccggaccatg ctcaaaccct tgatcgaccg gcaggatcac caactttccg gttccggcga
     2521 gtttccccga attcagcaac cgggcgatgt tcgtctttgt tcccgcgttg tcactctcat
     2581 accagctcag aatttcctgg acccgattgc tcataatcaa cctcccaacc aacctcgatc
     2641 acattagtcg ttacgcacgt ccttgcacat agtcttccgc cagactgaca tcctcagcac
     2701 tgccgataat gaggggaacc cgttgatgca gcgccttggg ctcgacatcg agaatgcgca
     2761 tcgtcccggt caacgctttg cctccggcct gttcgaccac catggccaac ggattgcctt
     2821 cgtacatcaa tcgcagtttt ccctcaggct tatccttctc tcccggatac agatagatgc
     2881 cgccgcccag gagaatccga tgcacatccg ccaccaaaca cccgctgtat cgcccgctat
     2941 aggggcgccc ggacggctta tcctgcactt tcagataatc gagataccgc tgtgtgccgg
     3001 caggccagcg atgataattc ccctcgttca ctgaatagac cttgcccttc gaaggaatgc
     3061 ggatgttctc attggacagg agatactcac cgatggacgg ttcgagcgta aagccatgga
     3121 caccctgccc gaccgtgaac acgagcatcg tgctggcccc atacaacaca taccccgcgg
     3181 cgatctgctc tgttcccttt tgcaacaact catcttcgac ggattgtccc gtccgcgatc
     3241 ggcggatgga aaaaatcgcc ccgagcggca tattgacatc ggtattggag gagccgtcca
     3301 agggatcgac caataacatg tacttcgcgc cttcgtggac catcttcagc ggcttttcca
     3361 tctcttcgga ggccaggacg cagacgaggc cgctgtgttc aaacacgcga acaaaggtat
     3421 cgttggcgat ctgatccagc ttcttgacga tctcgccttg gacattggtc tcaccggtcg
     3481 tgcccaggat cttgttcaat ccggcacgac gcaagtcatg agcgatcatt ttaccgacga
     3541 gaccgatttg agtcagaagg acggaaaatt cgccggtcgc ttcaggatgc gcggcctgtt
     3601 gttcgatgat aaagcggctt aacgtaatgg ggaatttcgc catgctgagg agtcctcatg
     3661 gattgaacga cgacgagtcg aaatgacgag ggcttcggac agagccgaag ccctccccac
     3721 gaacctggcg cgattatagc ggtttccggc cgagcgagca aaccaggccg gcagattatg
     3781 gctgcgcggc caacccgtag ccctccgtca attcagccac aatagaccct actacgacct
     3841 tcgccttcat ccgcacaggc acccggtgcg gatcggtggt aaaccagacc cggatattac
     3901 cttcgttcag aaaaataccc tgaaacggca tgatcaccac cacgcgggcc gtttccgcct
     3961 tgccccaccg gccctccacg gtttcaaggg cttcgacgcg cacgtccatt ttgtaattct
     4021 ttttgtcgtg atggaccgtc atcgtcaggg tttcccccgg cacgaacggc aagacgttgc
     4081 ggacatagta cagacaggac atcgcgtcct gcgcatccgg cggtatctgc agctcgtcgg
     4141 ttttgccgtc cctcacggcc gtcaccaggc cttcgtgatg ccggaaggta tagtcgaaat
     4201 cgttaaaccg ttttccctcc cggcgggcaa aggtcatgtg ggtcggcagg aatgagtcga
     4261 gatccacctg tgagaccccg cgattgtcga cgggataaaa cttcgtcacg gtcgggctcg
     4321 atcgtgcagt catgctcaag gtgagctgcc gccggccgcg ctggtcaagt ccttcctgga
     4381 cgatcatcgt ggcaatgccc gcccgcatcc ccagccaggt tacgtcgtag gcgagacgct
     4441 caccgttggc aaagggcagc ggcgcagaaa ccgatggccg tctgtccggg gttgattccg
     4501 cacacagcat cgatgggaga aggatgccgg ccacaatggc cgcgacaaac agaagagtgg
     4561 aagagagagg gaatcggagg atggtcaccc tagaccgaca acacacgccg cgcctgcgcc
     4621 agttcggttt cgatggtcgc gcggatcacg tccaacctgg ccggcgaagg cgcctcaaac
     4681 cggaggacca atgccggttg ggtgttcgag gcgcggatca acccccatcc atcctcgaaa
     4741 atcgcgcgaa cgccgtcgat cgtcaccaat tcccgcatcc ggagattcga cgcgccgacc
     4801 ggctggccgg cctcgagata ggaggctaac tgccgatgca cctgttcgac caattgaaac
     4861 ttcacggtgt cgggacaatc cactcgaatt tccggtgtca ctgtagtctg cgggagatcg
     4921 gccaccagac tcgagagcga gtgtttggtc tttgccagaa tctcgactaa ccggcaggaa
     4981 gcgtagatcg cgtcgtcaaa cccgaagtac cgatcggcaa aaaacatatg gccggacatt
     5041 tcgcctgcga ggacggctga ttcagccttt aatttcgcct tcatcagcga atggccggtt
     5101 ttccacatga tcggacgtcc gcctttggcc gcgatatcgt cgtacaagca ttgcgaggcc
     5161 ttgacctcag aaatgaaggt agtaccgggc ctctgagcga gaatatcgcg cgcgtacacg
     5221 atcatgaggc gatcccccca gaggatctct ccccgctcgt cgatcgcgcc gatgcgatcg
     5281 gcatctccgt cgtacccgat cccgacatcc gccttgtgtt cccgtacggt ggcaatcaga
     5341 tcttgaagat tctccacgac ggtaggatcg ggatggtggt tcggaaaacg cccgtccaac
     5401 tcatcataga gcccggtcac gcgacagccc atctgctcca gcgcctgctt ggcgactagc
     5461 gaggccgcgc cattgccgca atcgatgacg acatgcaggt gatctgcccg gacacccgcg
     5521 aacaccgtct tgagatgctg tagatagtcc ggaatgatgg gatgagagga cagttgcccg
     5581 gagccggaac tgaatcggcc gcgctccatg atgctccgca actgctgaat ctcttcgccg
     5641 tggatcgctt ccttcccgat gcagatcttg aagccgttgt attcggcggc gttgtggctg
     5701 ccggtgatca tgattccgcc gtggaccgga agcgtgaaca aggaaaaata caacagcggc
     5761 gatgcacaga ggcccaaatc gaccacatcg agcccgccgg caaggagacc ccgaagtaag
     5821 gcctgctgca acgcaggcga gctcaggcgc ccatcccgcc ccacactgat ccgcgaggcc
     5881 ccttgctccc tggccatagt ggcataggcc cggcccacct gctccgcaat gggttccgta
     5941 agctcttctc cgacaatgcc tctgagatca tattcgcgaa acagcgccat gccatctcct
     6001 aacgtgcccg gccgagtcgc ccgtaatcgt cctgaaaccg cacgatgtca tcttcaccga
     6061 gatagggccc gttctgcacc tcgataatgt ggagcgtctc ggtacctgga ttttcgaggc
     6121 gatggggagt ttcgaccgga attgcggtgc tctgcccgat ctggagatgc agcacgtcgt
     6181 cgccgcgggt cactcgagca gtccctgtga tcaccaccca gtgctcgctc cgcttatggt
     6241 gaagctgcaa tgacaatcgg ccgcccggat tgaccgtcac acgcttcact ttatatccag
     6301 gcccttcttc caagaccgta taggaacccc acgggcgaaa caccgtcaca tgttccagat
     6361 gctcaggcgc cccctgttgc ttgaggatct ctaccatctg cttcacgtct tgcgagcggg
     6421 atttcggaca gatcaaggtt gcatccggcg tatcgacgac gaccatatcc gtgaggccaa
     6481 tcgtggcgac gacccggcga tcggcgtaaa ggacggaatt gctgctgtcc atatcgataa
     6541 ctcgaccgct gaccacatta ccggccttgt cgcgcggcgc aacttcttca aggctactcc
     6601 agttgcccac atcggaccag ccgaacccca ccggaatcat ggccgcatgc gacgataact
     6661 ccatcacgcc gttgtcgatc gagaccgaag gaactttctt gtaggcggct tcaatctgcc
     6721 ggggatcggc gcccgatgtc atcagggtgt gaactttctg caccgctttc gccagctgcg
     6781 gctggtgacg attgatctcg tctcgaatcg tggcggcttt ccagaggaac atcccgctgt
     6841 tccagaagta attgccactt ttgagatact gcgccgcctt ggtccgactc ggtttctcga
     6901 caaatctggc aaccgggtga cctgtcagcc ggtccttctt ggctaaggta gttcgccggt
     6961 tcggttgaat gtatccatac ccggtttccg gcctggtcgg cttgatcccg aaggtcacca
     7021 agtgtccctt ctcggcaagc tgcatcccca acgtcactgc tgcctgaaac gccttctctc
     7081 cggtgacgac atgatccgcc ggcaacacca gcatcatcgc ctcgggatcg cgtcgaagga
     7141 gctccgccgc caccagcgca atggccggcg cggtgttgcg ccctatcggc tcaatgacat
     7201 agttgtcctt gagatcggac ttccattccc ctaattgcac gcgaatcgag tcggcctgcc
     7261 ctggattggt cgagatgagc acccgatcgg ggctggcaca ggagagcacg cgccgcatgg
     7321 tctgctgaat gagggtctcg tcgccgatga tgcgcaggag ctgcttagga tacaggtgcc
     7381 ggctcaatgg ccaaaacctg gtcccgctgc cgcccgccag aataaccgga tagaggtgat
     7441 ttgtcatatt tttttcgact ccgcttccgt tcaacatgac gcggttgttg ttctctcact
     7501 gggacttttg ggctgccagt atgagatccg ccaattcccg cacggcaccc tgcccgccgt
     7561 tcttcttaca tacgtagcgt acgaccttca acacgggagg catgccatcc gctggcgacg
     7621 cagagaaccc gaccgcaccc agcgcctgaa ggtcgttcac gtcatcaccg atgtaggcaa
     7681 cttgcttcat cgtgaggcca tgccgcgcga tgagatcctt caacactgcg agcttatcat
     7741 aggccccctg atgcacctcg ggaatcgtca atttttgcgc ccggcgcatg acgatttttg
     7801 tcgattcttg ggtaatgatc gccgtgacga ttccggcctt ctgcaacagc ttgatcccca
     7861 tgccgtcgcg cgtattgaac tttttccatt cgtctccgga ttctgagtag tacatgccgg
     7921 cgtccgtcag tacgccatcg acatccgttg cgaataaacg aatgccccgg agaatccctt
     7981 gaagggaagc aggtgacttc gatggagtgc tggttcgacg tgttgtgcta ggcatgcgaa
     8041 ggctttcggg ggcgatgtta acaagccatc acatgaaact caagaacact caatggcatg
     8101 cgatcgatgc ttgctgatca attaagaaac tcgtagagga gacgttggct ggaacgtagg
     8161 cacaattctt ttgagatcat gcagcaattc ctcctcgtca ctctctgcaa gtttcgagtg
     8221 cagttctgcg atccatcgct cgaacccttc cgctaccagc ggcgccccaa ccgcccggtt
     8281 aatcttacca tgagcggtcg ccaggacctg ctccccagct tcgaacaact cttcgtacag
     8341 tttttctccc acgcggagtc ctgtatagac aatttcgata tctttatccg gaacaaaccc
     8401 ggacaacaca atcatattcc tggcgagatc cgcgactttt atttgctccc ccatatctaa
     8461 gacaaacact tcccccccct gtcccatgac actcgcctgc aggacaagtt gaactgcttc
     8521 gggaatggtc ataaaaaatc gtttaatttc acgatccgtc accgtgaccg gaccgccctt
     8581 tctgatttgc tcggcaaaca atggcaccac actgccgttg ctgcccaata cattcccaaa
     8641 ccgaacaacg gtaaatcgcg tctgtcccaa ttggctcagc ccttgaatca catgttctgc
     8701 gatccgcttg gtggcgccca tcacactcga tggattgaca gccttatcgg tagaaatcaa
     8761 gacgaagcga tcgaccctcg acgccaacgc cgcctccgcg accacacgag tgccccagac
     8821 attgttccgc acggcttcct tgggattcag ttccatcaac ggaacgtgct tatgcgccgc
     8881 agcatgaaac acaatgtctg gctgagtttg ccgaaacacc tctgccacac gctcaggcat
     8941 tgtcacgtca gcaacgatcg gaagaataca gacatcggga aaggccgctt ggagttccag
     9001 caacaatgca tgcaaggcat tttcataccg ttcgaatagc accaaggttg ccggccggta
     9061 ccgggcaatt tgtcggcata actccgatcc aattgacccc cccgcaccag tgactaaagc
     9121 ggtttttccc tcgatcaacg ggtgtagttc ctgccgatcc gtttgaatcg gctcccgctg
     9181 gagaagatcc tctaagctca ttggccgaac ctgctgcaac agggctggat cttccagcag
     9241 ctgtcgcacg ctcggaagaa tcttgattgg caccttcgcg ccttcgacgg cagcaagcgt
     9301 tttttgcttc aaggccagcg gcgcagaagg gatggccatg aggatttcgt gcacctcaaa
     9361 acgacgcacg atttctctga tgttctgaat gcaacccgca atcggaaccc catggatttt
     9421 ggctccttgc ttcaccggat cgtcatccac aaacgcgacc gggcgatagt tgtaggcggg
     9481 gttcatctgc atgttccgaa cgagcaattc accggcgttc ccggccccca caattaagac
     9541 gcgacgagcc gtaggtccca cgatttgcag ccactctcga aaccaccgta caagcagtcg
     9601 aataccggca atattgaggc cggccagaag tccggtccca agaatgacag aacggggata
     9661 ctccactcgg cccaaaatcg gatgcaacag aacgaatgtc acaatcgtgc tgaccacaca
     9721 ggcccacata atccgcccga ggtcatgcaa gccgacatac cgccacaaac ctcgatggat
     9781 accgaacccc cacagtccga gcagatagac gacgcacacg agaggcatac ctctccacat
     9841 cgtgtcctta tagagggcgg gaatctgccc atcgaaacga aaggcaaagg ccgtgaaatt
     9901 ggcggccaaa atcaacccgg cctggacgca gattatcaat gccgtacgat aggcaagaat
     9961 tgtctggcca aatctcgagg agactttttg aaacacctgg acggctatgc gcttcatgcc
    10021 tgattcacac agatttcagc agtcagtgat taaccgatcg atctcaatcc gctcagatta
    10081 tcccgagcga gcacccgcga gggttgccgc gcctgaaaca gcggccacta caccctccct
    10141 gtccgattcg cgttgtgagt taacatgaag gaccgcgatc ccaaccacaa cccaaaactg
    10201 aactgcaaga gtaccgacca gcatctgatc aaacatcagc cgcacgccta gtccgatcac
    10261 accgaccgtc gctcccacca caatggctcg agctgtcgag ccttccgttc ggtgacattc
    10321 ctggatgagt cggcgaacca gtttccccca caaccacaga aacaacacca taccgatgag
    10381 cccgacatgc agcgccgacg cgagcacaat gttgtgggcg ccggccgtct tcacaggatg
    10441 atgcccaggt tgcacctctt ctggttcatc tccatacacc aacttgtagt tgtcttttcc
    10501 gtagcctatc cccacaaacc aatgctcctg aatctttgac aaagcaaacg cccaaatatc
    10561 gaaacgatga atcacagacg cgccatcagt tcgaaccgga gtgccatccc gtaaagtaag
    10621 acgaagttga ccatcagaac cggtatccac gcgaattaga aacagggcag cgagcaagca
    10681 tactaccgtc gcaccacccg caacggtcac tgcgctacga cgcttgagca accaggccat
    10741 gacccacagt tcgacaaaca gcgccaccaa acccgcacgt gatcgggtca tcagcagaca
    10801 gatcgtgacc aaaatgctcc cgcatccata cacgtagcgg agacgattcg tgacttcgta
    10861 gtaggcaagt gagaaaaaca gcggccccag aagcacaagg taagttgtca gccacacctc
    10921 ggcagggaaa aatgaccgag tagcctgacc gtcataccac tggaactgtg taattccata
    10981 ggcactcacc accagcacac acccgagaag aagatacgtc agaagatcgc gcgcggtgcg
    11041 ctccctgaag aaatacagca ccgcatagaa aatgaccacc tgctgtagga gtttgccaaa
    11101 ctccttcatg ctataggcag gatgtgccga aaagggaatc gtgaaagcca cccaagcaac
    11161 aaacgcgatc aatggtaaat cgacggggct agacacaaaa aagtggcggc gacgaagtag
    11221 gcaccacagc ggcaggagca ccaggagtac taagaagatg ttgcgttcaa ccactaagag
    11281 tggcaggaac ggcagggaga aaatatacac ataaagcaag gcccggacaa tcagatccag
    11341 agttggtccg tgatttgctt ccatggaggc ccatacagcg ggaatccgac taatcttcat
    11401 acattcgccc ctgctgccca gcacgctgct ccatcagacc gactcccaca gccaacccag
    11461 caaaacccag tatccagacg accaaaatcg caagccgccc cgaatcggtg gctccgtgat
    11521 aaagcactgc ggcggccacg cacagtgcca tcagcgcata ttctgacaga agcgtttttc
    11581 gatgtcccca cccgtggaga accaaccgct ggtaatagtg ggttcgatgg gcttcccaaa
    11641 ttttctctcc gcgcacggcc cgccccaaca tggtggtggt tgcgtccagc acaaacggcg
    11701 aaaacaccaa gagtggcgtc cagatatcaa acaggccatc ctgcacgccc aagacactca
    11761 atgtgccgct taaaaagccg agcgaaatac tgccgacatc ccccatgaaa atgcgcgctg
    11821 gaggaagatt atagagaaga aacccgacgg ctcccatcga aataatgagg gaaagaatga
    11881 gaatgaatgg atgttgtgcc tgccaggcaa agtaggctat cgcaccaaat ccaaacacgg
    11941 tcatcccgcc cgcaaatccg tccattccat ccataaaatt gtaaagattc gtcatccaaa
    12001 ggacaaacaa gagagtcagg gggaccgcaa cgccctcgaa ggagaggcta ccgacagcag
    12061 gcagggacag agacgacaat gaaacgccag ctccgatgac gactgtcgcg gcggcaatca
    12121 gttgaacggc taaccgaagg agcacaggca atccgtcacg gtcgtcccag aacgacacca
    12181 ctgccagcag aactgtcatg gccaaaatcc acgcccctgc cgttggggaa gtctgcgagg
    12241 cgcttaacca ccatgcctgg gtaggaaatt cccagagggt tgctactatc aacccgatgg
    12301 ccgcgctaac aaggagcgcg aggccgcctg tgcgtggcgt gggaatctca tgcagtgatc
    12361 gcgcattggg gtagtccaac agataaagac gagaaccggg catgcacaac caaccgattc
    12421 cccagtacgt caagacaagc acgaggccac cgactcctaa cagcgccact tctcgattcc
    12481 ttccagagtc agccagcatc actattgatg ctgaagataa tattccgccg ctcggcgcaa
    12541 ttcctcgtcg acacgtatcg gggggcacca ccccagcaag gtacgcgctt ttgtaatatt
    12601 cactcgtaga ttcccgcaga gacgagcagc catctctcgc tgaccgagca gagttaatcc
    12661 cgatctcaat gctggcatgg gaaccggaat gagtcgtgcc ggacgattca tggcaagggc
    12721 cagccgatca atcaattctg tcgtggagag atcctcacca tcgctcacga agaatatctc
    12781 attggcagcg cgtgggtgct cgatgcaagt gacgatgaaa tcaacgagat tccccaaccc
    12841 cactagactg cggcggttac ggatcgctcc tagcggcaag ggaataccac gggccagcgc
    12901 ttgcatcaac aactgaaaat tggcccttac tcctggcccg tagacgagag gcggccgcac
    12961 gaccaccacc ttgagggcgg tatgcgcggc tatctcccgc aaaccgacct ccgcctcgta
    13021 cttgctcacc ccgtaggcgt cctggggtgc cggcacatcg gtttcgcggt acgcgtcctg
    13081 tccgccctct ccattgacct tcacagagct cacgtagatt agccgctcta cccccagttc
    13141 cgccgcctga cgcgcgagag cgaccgtacc gcagacattg gccgtgcgaa atgctggcaa
    13201 cggatctgcg tcggtttctt gcatgacatg cacgcgcgcc gcgcagtgca cgatcacatg
    13261 cactccagtc agagcctcgt gccagtcggt atcggggccc agatcacgaa cctggcaata
    13321 ggccagacca cgcactggct gggccgccgg gcgccgtacc gcggcagtga cagtcacgcc
    13381 ctgcgcgacc agttgagcgc agacagcgtg tcccacaaag ccggatgcac cggtcaccaa
    13441 gacgttcacg gcgtgagttc cagataaaca gccagggtct ccttgatgat ccgctcttga
    13501 gaaaactctg ccaacacaat ctcccgggca cgtcggccca ttgtggcacg gagcgctgcg
    13561 tcttcaatga gtctggccag ggccagagcg agagcttgcg cgttacggat gggaaccaga
    13621 aagccattgt caccgtcgtg caccacatcc cgacatccag gagcatcggt ggtgactagc
    13681 ggaagtccgc aggccgccgc ttccaacagc accttcggca gcccttcccg gtacgaaggc
    13741 aggcaggcga tgtgcccgag ttgcaagacc gcaggcatat cttcgcgccg cccccaccac
    13801 tcaatgccgt gctgaccatg ccaggcgcgt agggaagtat ccggcactga ggccggattg
    13861 ccgggatccg gatcacctac cagcacaaat cgggccttca cccctgcatc cgtcaacaca
    13921 tgcgcggcct caacgaattc acgcaccccc ttatccaaca acattcgcgc cgcgaatacg
    13981 accgtgacgg gctcagacgg caattgggaa gggggagcaa acacctccgt atccacccct
    14041 gcaccgcgga tcaggcgcag acgatcttcc ggcactccct tttgggtcaa caaaccaaaa
    14101 tcgtccgggt tctgtacgat cgtgcagctg gacgacgccc caagcaacca taccagcagc
    14161 ctgcaaaagg cggggcgcaa agccttgacg gcaagtgtat ttgacgtgaa taaccagccc
    14221 agtcccgcaa cggcattgac cacatgaggc acggcagcga tcttagctgc aagcgcccca
    14281 tagagcaccg gcttcagtgc gacgtgatgc acgatgtccg gccgctcccg ccgatacagg
    14341 cgcgcgagca tggcaatttc ttgcagcgga ttgccgcggc gacgggagag gtgacaggga
    14401 ataaggcgaa tcccctcagc ccgaatgcgt tctccgtgct ccccttcgcg ggtagccacc
    14461 accacgtcga agcccgcctt ctgagctgcc agggcgagcg gcagccggtg ggagacgaaa
    14521 taccagtcct cggtaacgac aaagagtaat ttgggacacc tactcatgca tgcctacggt
    14581 gggcatacgg ccgcgtcagc acggctccta acacgagcca aaggatgcgc tgataaaata
    14641 tttcgtgagc gaggctaaat acggcgaagg ccaccagcag tagcaacaaa atgcaatttg
    14701 cgggctgggt agttcccgac cggatcgcgt gatgggccag caggaaaaat gtccagccga
    14761 acacagcgcc tcccagcagg ccgaactcgg ccagaatcca caacggtgtg ctatggatga
    14821 cctgtgggtg tcccgcccac atcggactct tctcgttaaa cactcctaat ccgacaccca
    14881 gaactggcga ttgctgccac atttccaagc cgtgggtcaa tgtttcccaa cgctccagat
    14941 tactgctctc gtaagatagt ttactctgga cagacatgtt tccatatttt tgtggcacca
    15001 ataggactat ggaccagagc agtgccgcga atatcatccc cagccgagca ttttgtggtc
    15061 ggccatgccg ccgagccaag caagaaccag tgccatcgct cccaccagca tagccgttcg
    15121 cgaaccggtc cagaccagac cagccaataa gatgccaagc agcaaagaaa atatccatgg
    15181 tcgtactgag cgtggcacac gaatgttgtg tcttgcatac aaatgagaat agcccaaaaa
    15241 gagcgccatg cccgccagca cctggagcgc aaaagcattg cggttccccg catacccctc
    15301 gaagttttgt gtaagttgcg cggcggcatt taccccccag tgggacaaaa gacgcagggc
    15361 gacttgcaac atcacgacca cgacagcagt agcaattaga gtttcggcga agcgccgcag
    15421 tccgtgagtc ccgacgttcg ccacgatcac ataccccgac gaaagatagc caagcagcac
    15481 caaccacccc aggagccgcc ctcccagcgc ccactgcgtg atgcctattt ccaaccaacc
    15541 gtgtacgaac cccaccagca acagtaaact gatcacgcac aacgcgaagt tgaattgggg
    15601 aactctccat accggaaact gccgggcgaa caacgcatgc aggctcaccg ccgccagagc
    15661 cagaatggcg aagggatctg ccaggttcag gttgataggc ccaccagaca gggatacgtg
    15721 aacctgaaaa aacaccgcga ccgccaccac cgtacccaaa acccaagcag taaactcttc
    15781 aatctccgac accgaatgaa tcatcaccgc tggtggttta ggcaaatcag acatgagaga
    15841 gtttctgaaa cagaacggtg aggacgcgag aatgaccagc gtagaacaca accccaccat
    15901 gaccgacatc gccagggcat ctgccgcggg gatacccaga aggccgaaca cgtacaccgc
    15961 cgcaacctcg cgcacgcccc aacctccgac agtaatcggc atgctggcag ccaagctaat
    16021 aagggccgac gcggcgaaca aggatgcgat gggaatatcc tggctgacgg ccagaatccc
    16081 tactacgaaa caggccagca tcaggagctg gcctgtaagg gtcagacctg cgattgacat
    16141 tacatgttca agattcgtcc acgacagcgt ccgtctggac aatttcctct caaacctcga
    16201 acctccaagc cagagactca atgcccaacc cccgattgcg gcaaccacaa tctcgggaag
    16261 tgcgatctgg ttaaaaaaat cactaaccgc agatcttccg agcaaataga ccgcgcccag
    16321 agtcccgagc actccgctga gaagcgccag cagggcacgt tcataggcag ccagactggc
    16381 attcaccaca ggttgtacgc caaaactgtt cagcactcct tggcgtccca tgatctgtcc
    16441 gaacagcgga atcacgaaga gcccggcgac ggtaccggca atgctggcgc gcaaagcgat
    16501 gtcccagggt agcgcaatgc caaaatgcgc caatacccgc caaaaccgga acgataccac
    16561 aaacagattc gctgtcagca atgccagcat gcttgccaca gcccagggag gaaatccggc
    16621 cagcctccgc aagacgtcct caacgccagc tgaccagagc acaaatgcga tcagaaatat
    16681 cgagacaaaa accgcgggca ggcgacgaaa ggagaaatta ctgagcaaga atatgagctc
    16741 cgttgtgtgc cgagtcgccc atactctcca accatgcctg gaacgagagc acatcccaaa
    16801 ggtaataggc ccaattccgc cggccagtca ggtgttccag ccatttcgtg cgaatcgctt
    16861 gagggtcaaa atagccctcg cgttgtaggc gcgactcatc caataactcc tcggcccaac
    16921 ctttcaacgg tccacgcagc cacacatcga tgggtacacc aaaccccatc ttggggcggt
    16981 cgatcagtgc cttcggcaca tgccgataga gcatctggcg cagcaaccat ttgccttggc
    17041 catcccgaat tttcatggat aacggcagcg accaggcaaa ctccactacg cgatgatcca
    17101 gaaacggcac acgcgtctcc aatgacacac ccatcgccgc gcgatccact ttcgtaagaa
    17161 tgtccccggg caggtacgtc atctggtcaa ggtacatcat gcgccgctcg aaatccggca
    17221 ggttcgccca ttgcgtctga tcggtcaata ccgtgggcgg ctcatgtgcg ccgcgaacca
    17281 cttcggcagg atgcttccag tgtgacagca ggtcaaagta aatttcctct ggcgtcgcca
    17341 ccgccagtgt ctcggcaagt ttgtgtaact tgtccccggg attggcatag cgccatccct
    17401 ccggcagaaa gcgggcgaac cgctggaata cactattcca tgcggaaggc ggcagagccg
    17461 tgagcgcacc tgccaacgcc gtgcgtatcg attggggggc ccacccgatg cggcgccaga
    17521 tggttgtggc ccaaaaataa cgattgtatc caccaaataa ttcatccgca ccatcgccgg
    17581 agaggctcac ggtgacatgc cgacgagcca gttcgctgac taaacacgtc ggaatctgtg
    17641 acgaatcaga aaatggttca tcgtagaggg tcggcagccg gggaatgaca tccatcgcct
    17701 ctttcggcaa gacgtacaat tctgtgtgct ccgtcccgag atggcgcgcc acgagcttcg
    17761 cgtgtgtcgc ttcgtcgtac ccctcttcat ggaaaccgat ggtgaacgtt ttcaccgggc
    17821 ggcttgactg cgcctgcatc aaggccacga cagttgagga atctattccg ccggaaagaa
    17881 atgcgcctaa tggcacgtca gcaaccatct gccggccaat ggcttccttt aacagggctt
    17941 caagccgaga catcgcctct gcttcacttc cctcaaaggg atgtgcgaga cctgcctcca
    18001 cgcactgccg gctactccag aaagccttcg tctgcggcgt tcgctgtgac aaagacaccg
    18061 tgagaagaca cccgggcacc aacttggaaa tgccctgata aatggaatag ggcgcgggaa
    18121 tgtaattgtg ccgcagcaat aacgccaaag cgtcccggtc tatctcggcc cgaaaggccg
    18181 gatgcgcctt gagcgacttc agctctgagc caaacagaaa cgtttgcccc tgccagccat
    18241 agtagagtgg cttttccccc aagcggtccc gccccaaggt cagggtttgc gtgtggttgt
    18301 cccacaccgc aaaggcgaac atgccgacaa atcgcttgac ggacatctcg atgccccacg
    18361 cgtcaaacgc agctaacatg acctccgtat cggaatggcc acgaaacgtg gttccagctg
    18421 cttcaaactc cgttcgaagc tcaccgaaat tgtacacttc gccgttgtag gacaacacat
    18481 agcggccgct gcgcgacgac atgggctggt gcccctccgg agacaaatcc agaatcgaga
    18541 ggcgggcatg accaagtcca acacctgcag cgggatcgct ccagatgccc gaatcgtctg
    18601 ggcctcgata ccggatcgct gccaccattc gcgcgacggt cgcatccaac tcacctcgag
    18661 attggctgag aagcccggct atgccacaca tagggtacgc aacccgtttc tcataacgtc
    18721 caatatttgt cagcacatcc gttcacgggg tgatcgggac tgcgccgcga gctcggtata
    18781 gaggacttca taacactccg cgaccttggg cagtgcgtat tccgcctcaa tgcgtgcacg
    18841 cgccgcatat ccgagtttcg cgcgccccgc cggcccacga tcaatcaggt cagtcatcgc
    18901 accggcaagg gcagcaggat cgcggagcgg caccaccagt ccggtgtcac caataatgtc
    18961 acgagcatcg ccaacatccg tggagataca aggtatgcca caggccatcg cttcgccgag
    19021 gacgtttgga aacccctcgc ccagcgaact gagacacccc acgtcgacac caggtaacag
    19081 ccgttcgata tggccgtgat gccctaagag cagtacatga tccgtcaagc cccttttagc
    19141 gacactgggc gcgagcgtct ctgtatcctt tcctaccagg acgaaatggg ctttcttttt
    19201 cgcatctacc actcgcttgg ccgcatcaag aaacgtggcg tggtctttca tcggatcgac
    19261 ccgcgcaata aacgcaacga gaacggtatc gtctgacagg ccccactcgg ttcgaatcgc
    19321 cgctcgagca cttcgatcag gacgaaaccg ctgcagatcg aatccattcg gaatcaccac
    19381 ccagcgccgt gggcgatagc ccaaacgggc atgctgctgc ctccccgcta cgctgttggc
    19441 gatgaccgca actggcacgt gggagagcca gaccagcatc cattgcacga ttcgcgtgag
    19501 acgcgagtac cctgtgagat ccatgtgcga gcagcgtagg ttccagatta gaatgggcga
    19561 gcccgacacc ttgacagcca gcgtactcag tagatcggca tgatagagcc aactttgcac
    19621 aatcgcgggc tgcagtgtcg tgaacaatcg ccgtaaccgc acgagcgcgg aaagattggg
    19681 acacccgcgc ttcatcccca gcgtgtggag cgcaatgccc gacgcttcga tctgttctcc
    19741 caactgtccc cggtctgtaa gcgaaatgac aatattcgtg aaccgtgtgc gatctagtcc
    19801 ttgcaacacc ttgacgagca tcacctcggc gccaccggta tcaagaccgg agattacatg
    19861 gatgattcga atcatgctga cagagagaat ccgaactcac agggactctt ggactgtttc
    19921 atcaccgatt ctgataccgt ccccaagggg tcaccgtcgc accatggtat gccgtccggg
    19981 aatcggaaga cgaccgtgaa acaagagcgg cctgcacatg tcatgcacat cccgacaacg
    20041 gtcatcgcgc agtcgcctca agaacggagg ccgtatcacc gatcaaccga tcaagactga
    20101 aatgctggat gacgcgatcc cggcccatct gcccttgctt tgcccgttcg gcgccatcca
    20161 ttcgtaacaa ttcttgtatg gcacccgcca gggcctctga gttgcgagat ggaacgatgc
    20221 ctccaatctc ccccaggatt cgcttcgaat caccgacatc tgttgcaaca cagggcacac
    20281 cgcaggccat ggcttcaaga agagtgttgg ggcacccctc ggcggtcgat gtcaggcagg
    20341 caacgtccag tgcgttatag actgcgggca tatcactatg ttcgcctgcc caaatcacgc
    20401 gggctgacaa agccagctct gcgcacagcg cgaccatcga ctccttgtat gccgaatctc
    20461 cccctccaat gcacacgaat ttcaccagag cgcactgccg aacaaccagt gcagccgctc
    20521 gcagaaaggt ggggtgatct ttaataggat cgagtcgccc gaccaacccg atcagcacct
    20581 cctcatccct gatcccccaa gagcatcgaa cggtcttacc cttggatcgg tcaggcgcaa
    20641 acagttcggt gtcgatgccg ttgggaataa ccgagattcg atccgtgcgg tacccctcca
    20701 ccttcgcata acgaaggcca gcttcggaat ttacgatgat gtgatccgcg aatcgggaca
    20761 accgcgcttc aagccaatag gagacgcgga cgaggcgatc atagtgcgtc aggtccatgg
    20821 aggcggttcg aatcccccat gtgactcgca gcgtgggaac gaagagtttc tccaccgctg
    20881 ccaccacatt ggacacctgc agcaggctgt acaccacgtc cggacgctct cgccgtaagc
    20941 accgccggat tctctgtaaa aatccaacca actcccagcg tcgcagtttc ccgacacttt
    21001 caacgggaat tccagccgcc aataattcgt cgtacaagtc cccgcctgaa tagaagacaa
    21061 ggacaccgac atcgtggcca agctggcgca acccttttgc aagcgccacg cactgccgct
    21121 ccgctccacc ccgctgcagc gaccggataa ggaaacgaac tttcacaggc gctctgtgga
    21181 cacgcggtga aacacgaatt cattgcagcc gataccaccc gcgcaggttt tcattcgctg
    21241 caaatcaaac ccctttttcc gatagaaatc gaaaatctcc tctggtttgg ccacctcgaa
    21301 tggatagcct ccaacccaat caatcacatc ccgccaagga gacattcccc gttgttcctt
    21361 gtaactaaac cacgtccgaa gtgggttgcc caagaacgtg tcacggacga cggtgagcgt
    21421 ccactgacga atacatgcca gccatgccac cagaattttc ccccattgag gggaccgatt
    21481 atacacacgc ttcaccatta accaccggcg gctggccccg ccctgatcgt tataaatggc
    21541 gataaagagt gttccctggt cagcgactaa acgggaggca ttgtccagtg cctgccacat
    21601 ggctccggtg tgatggagca caccccagga gtagacgata tcgtaggttc ccagcgactg
    21661 cacgtaggcc atgtccagga tagacccctc ttcaacgatc caggtgtcat catcccggaa
    21721 atagcgccgc ttcagttcag cggtgcaggc agcagaatgc ggatcatagt caaaagaacg
    21781 aacggaggcc cccagccttt tggcggccag actgaacaga ccgctacccg acccgatatc
    21841 caaaaagcgc tgtcccgcaa ggctcttcaa cccgagcatt gcttggagcg attcctgggc
    21901 acgaccaatc cgctcttcag tgagatcctc tagaaacctg cgccagttct gcccaaattc
    21961 aaagcgacgg ccatccgcga cttcctgtgc ctgttgcgtc atcatgctcc ccgtttcact
    22021 atgcgatcgg ttcgaggatc ttctcccaca tccccataac tttctccacc ccaaaccgct
    22081 gcgtgatgtc catcgcccga ccgcctaatc gtgctctccg ctctggatct gacatcagac
    22141 tatccatcgc agtcgccaag gcgtccctgt caccaggcgg gacgagaagg ccatcctcac
    22201 catcacgaat gatctcgcgg ggcccgctcc gacaatccgt ggcaatcact ggaagtccat
    22261 aggccattgc ctcacacagt gcattgggaa acccttcgaa gcgggagctc aacacaaaca
    22321 tacttgcttg ccgcagaaac acttctatgg tggtcacgag tcccggtagg agcacgcatc
    22381 cggacaggcc gagcctctcg cacaacaatt ccagttctcc gcgctcaggg ccctccccta
    22441 tgatagtgag cgtgcagccg gggtgctgat cgttcacctt gcgaaatgca tacaaaaggt
    22501 catcaaaccc cttctctttg gacaaccgac ccatggcgag aatcatgcat cccgcagggc
    22561 gatggttcac catcgcatag ttgctcgagg acggcaggac gggattggga atgacgctga
    22621 ctgtacgctg aaattttggc aagaaatagg cccgggcagc atgggtctgc acgataattc
    22681 gatcagctcg agagtacacc caccatcgta gcgcgcccca gatccgacca attgagtatt
    22741 gataggggtt cgtacgctcc gaaaccatga cttcaagctt caaccccaga gtcgcaaaga
    22801 gagtcagcac attggttttg tcgacaaaac tgatcacggc gtccggctgg cttcgtagaa
    22861 tcgcctgacg tagcctcacc actcgattga tgttattccg aatccccgcg atcagtccgg
    22921 ccgaatctcc caaaaggccc aaagacatcc ggcgcactga gggatcgacc tcgaaaaatg
    22981 gcggggtctc gccatcatcg aaagacaaaa tggtgacgcc ccaccctttg cgagcccaat
    23041 aattggccat gatggagatt acacgttccg ccccgcccgc aaaaagagct gatatgacaa
    23101 acgtgacttt catgccctca accccgaaca tggcagccgg tcgcctgctg actcacgcgc
    23161 agaccaacaa tcacgccctc tttgctccca gatgctggta gaggcgcacc aaatccgaaa
    23221 ttgttcgatc gatgtccaga tgctgacgac aatattcaac gccgcgcaat ccgattcgcg
    23281 cgcctcggtc aggaaggctc aacatctcca ccacggcttt tgcaagggct tccgaattcc
    23341 tcgcgggaac caacacgcca tggccactct tccccagtag ctctgtttca catccggtat
    23401 caaatccaac aactggaagg cccattgcca tggcttggta gctggacaaa ttttcacttt
    23461 caaacaccgg cgtcctcaga taaatatcgg cggccgcata atacggcaat gggtcccgct
    23521 gaaacccaag caaatgtaca tgttccctta ccttgagttc gctcgcacga gcctccaatg
    23581 cctctcgttg ttctccttca cccaggacta cccaatgcag ctggggaaac tgagccacaa
    23641 gctccggcac cgcctcgagc gcaaacgcat gccctttcga cgggtgcagt cgaccaactg
    23701 ataacgcgat aaccgcgtcc cccggtaact tcaactcatc tcgcagggca gtgtagtagt
    23761 gctgcttctg ctttctcatc gaggtgcttg aggttagatc aacggtgcca tggataggca
    23821 tgattttact gtccgggatt ccgatcgaat gcaattccgc tacggaggcc ccggtgatga
    23881 atcgggcaac ccattgctga ttcgaccgat agagctgagg tattccgtgg aatcctgcat
    23941 ccgtcatttg cgaaaacaag gctgggacag aatgtacaag gggtatctgc aacattcgcc
    24001 gtaagactaa tcccacccta aggccatgaa acgtatgcac gtgaatgaca tccggtcttt
    24061 tctgaacgag cgcaacagcg acccgccacc acacatccac tgcattgcaa aaccaccaaa
    24121 gtccggaaag tgtcttacgg ataggcaaga ggtaccactg ggaggcaaca gtctggatgc
    24181 ctcgttcaag ccattccaag aaaggatggt gcgcgggttg cttgtaggcg cctcgactgc
    24241 gtaacggctc agaatagacg aactttgcgt tatcgggaaa gaagctaatc acgaaatgct
    24301 tgcacagagt cggctcagaa agacggacaa tatcccacac ccaccgctcg gaaccgccgc
    24361 cgcccagtcc gtcgataagg tgcaccacgc tgatgggatc ggctgatgtt agctctgcgt
    24421 gcccttccgc catgtcaatt tacccagttg tttcaacaca ctcgtgacat cctcggatgt
    24481 gagcaagagt gtttcgcgta gggttgcaga ggaagttatc caatagagac cgaacaccac
    24541 aacagcccct acggcttggg tcaacgccaa agccatggcg gctcccatct ccgcccatgt
    24601 tggaattagc cagtaactcg acgccagaca aatgcacgcg ttcgctccag ctagcagaga
    24661 gaggagacct ggacgccctc gctggcccag aacataggtg ctgacgatca tggacacact
    24721 cacaaatacc actccgacga gaaggatcca gaacacatac acggccggct ggtaggatgg
    24781 tccaaacagc gccatcacaa tccacggtgc aagaagcata gctcccaccg tgctggcgac
    24841 catcccagac accacaattc gtatgtagcg attcattgcc gcaatcgctt cgcgtggcga
    24901 taacgccgac attttcccat agatggcgtc ttgcgcggcc tggatgggca tcaacgctct
    24961 ctccgccaat tgctgcgcca cggaaaaaat ccccactgaa gcggctccgt gaaatgcatt
    25021 gagcacaaac acgggaatac gcatccaccc catggtcgca atggcattgg gataggcacg
    25081 aagaccgaat gccagcatct gtcgcgataa gcccgcctca acatactcgc ttccgccaag
    25141 aaatcccaaa cgacgcgata gcgccaccat gagcacgcat tgcagcacca tggtagagag
    25201 atagattgtc acggccgagg ccactcctcc ggaaaacccc acgacaagca cgagaatgag
    25261 aatgagctgt acgggaccta tcacgagttg cacgccattg agaagcggaa tttctcctaa
    25321 tccgatcatg aggctattcc agaaccccgc atacagcatg catggcagga ttgcaaacgc
    25381 cgcccattgc cagcgcgctg gaaaatcatg caggagctgc tcaccggcca tccacagcac
    25441 caacccaccg attccactca tcccaatcca caagatgatt gccgccttac tgacaaatgc
    25501 atggatgagc tttttgcgat gggcactccg cccgacaaac acattggcgc tttgtgagag
    25561 tccacaatca cccaccacgc tgagcactga cacgagcgtt tgaaagagaa aatattcacc
    25621 gcgcccttga ggctccaaat atcgggcaag cagaaccaca ctgatcacat tgatgaccag
    25681 gagaatcact cttgccccaa gcccgaccag tgaaagtttt agggtagaca cgcgggtgat
    25741 cccgaagagc tgctgtggcg cctggactcg ggttcctggg tgcggacagt cggattacac
    25801 actcaggtat gcacagaaac tgaggctatg caggaccatt gaaggctcac ctccgcgcct
    25861 ttaagaggcg cgtcactgtt gccgtgacct gggtgacctg gttttcggtc atcgccggat
    25921 aaaacgggag ggacaccacg ctatcgccaa ttttttcggc atgcgggaaa tcgccgacac
    25981 gaaacccgaa tgtgtcccga aagtagctca gaagatggat tgcgcgataa ttcaccacac
    26041 tgccaattcc atcctcctgc aacccttgga tgattgcgtc tcgtcccccg ccctcaacaa
    26101 acacgggaaa gaggtgcctg gcgtgacgca cctgcggatg agattttggc accgacacac
    26161 cagggcaatc cgacagcaag ccttcatacc accgggccac ggtatcacgc ttggaccaat
    26221 tctgttcgat acgatccatt tgaggaagga ggagcgcggc gtggatatta tccatgttgt
    26281 acttccaacc cagcacggtc atgtcccagt gctgataacc ctcgcggtga cgatctgctg
    26341 cggtttttgt catgccatgg agcctgagca ggcgaagctt ttcggctagc ccccgatcct
    26401 tggtcacaag agcccctccc tctccgcagg tcagattctt ggtcgcataa aaggagaagc
    26461 acgccgtatc tcccaactca cctggcttca ccccatcacg aacaccttca aggcaatgag
    26521 cggcatcttc aatcacaaac aggccatgtt gatcagcgat tttgcgaatg gtcaccatat
    26581 cgcacatctg tccgtacagg tgaacaggca gaatagcttt tgtccgtggc gtaatcgccg
    26641 cctcgacttt ggtcgcatct aaattgcccg tctgcgattc cacatcaaca aaaactggct
    26701 ttgccccggc ttcgagaatg gccgtggagg acgcaataaa ggtcatcggc gtcgtgatca
    26761 cttcgtctcc aaggccgatg cctaatgcca ggagggaaag atgaagcgca ccggtacaac
    26821 tcgtcacggc cagcgcatgc ggtacaccaa gataggccgc aaagcgtcgc tcaaacgact
    26881 cgacgacttc ccctgttgtc aaaattggcc ccgcgagaac ctttcgcacg gcctcaactt
    26941 ccggttcgcc taaatcatgg cgatagaaag gaatgcccat ctcagtgtag ctcccgctta
    27001 cccgccgcgg tgacgatggg aatccgtctc atccacatct gcggtctcca ccaggcttcg
    27061 ttctcccgat accattgaat cgttcgctcg aggccctgct cgaatgaggt gcgcggctgc
    27121 cagccgagaa tccgcgagac cttcgaatga tcacacgtgt ggcggaacac ctgtccggga
    27181 cgatcaccga taaattgaat ggggctcttc ttcggcttca tcagcgcccg aacggtatca
    27241 gcgatctcca tcagactgat atgccgccct gtccctaaat tgagcacctc accaaatacg
    27301 cgctttggct ccgcgtgcag aatcaaatca agcgcttcgc aatgatcttg tacatagata
    27361 aagtctctcg ccgcagaccc gtctccgtga acggtaattg gctcatcgag aatacagctc
    27421 gtgatgaagc gcggcaccac cttctcaagg tgttgatacg gtccataatt attgaacggg
    27481 cggacaatga tcgcagggat tccataggtc tgccaatacg aatagaccaa tcgatcagcg
    27541 ccgcacttcg ccgatgcata cgggctcatc ggaagcaatg ggtgctcctc cgacattttt
    27601 tctgattccg cggtcccgta gacctcagat gtcgagatat gaatgaaccg ctctacttta
    27661 tcacgatatt tgagtacggc gtttgcaaca gcttgcgtgc ccaacacgtc ggtctcaaaa
    27721 aagagcaagt tgtcataaat tgaccgcgtg acatgtgact cggcagcaaa atgcatcacc
    27781 acatccgatt gggataccac cgtatcaacc agctcggcgt tccgcacatt tccataccag
    27841 aacatcagtc gctcgtcgga accattgtta atgtccaccg gcagattctt cacattcccc
    27901 gcgtaggtca aggcatccaa cacgatcaaa cgatagttcg gatacttgtc gtagagatag
    27961 cgcacaaaat tactgccaat gaaacccgcc cctccggtaa tcagtatggt tttcaccaat
    28021 cctccttttt cattggccgc gtaagatggc cgcgctatcc ggcccacaat tcaacacgag
    28081 atcgagagca gagagaaacg ggacaaactc cccgtgctgt tgtggataca cggggtgctg
    28141 aaaaccctgc cactcaacct caattccatt ttgcgcgaag agctcacggt ccagataggt
    28201 cttcgccgcg tcaccgctga gataccgtgt tgcgccaaaa tgtctacaca gtgcgagcaa
    28261 tctgtcactc cgatctcctt caatgccgag cgaggatgca cggatgacgt cgcgcttaag
    28321 ttgcaaccac tcacacatta agtcgatcag agataaatca aggtcaacca gtaacgtcca
    28381 gggccgtaac agtcggtctt ccagcatcgg caggtattga gccaaataag gcgcctttgc
    28441 ataaaactgt cggatcgttc cgatatgctt tttcccccag actgtctggt tatcaatttc
    28501 aacttcgttg atacgtggcc accctaatcc cttatggcgg acggggacgg tcaaccaatg
    28561 aggccccttc ggagtcttga tacgattccg atttcgccat ccgtgcttgt cgtactgaac
    28621 atcgtcatac aggacgaaga tatcacttcg acgcatctga tcaaagaagc caagccacgg
    28681 caaatagcca ggctgaagta cacacagcgt gatggatgac tgaagattcg gatcgtgcat
    28741 tattcaggca ggaatactcg cactcttagc gttagattcg aaccatggac cttaaaccat
    28801 atacaagtca taccactcct gaaacacaac caatgcatac actttgttga cggcgagatg
    28861 atccggctgc gcctggtata acgcatccac cagccgctga acgtaggccg cacgaaacaa
    28921 cccgtgtttg gccagtcgct ctgggctgag ggtctctcga acatatgact ctagatcccg
    28981 tagcagccat tgcgtaatcg gcatcaaaaa gccttccttc ttgcgataca ccatctcgcg
    29041 cggaaagtat cgtagtccca gcttcttgag caagtacttg gtttctccgt ctttgatctt
    29101 cagcgatccc ggcagtccag ccacaaactc gacgaagtcg gtatcgagaa aggccgtgcg
    29161 aacctcaagt gaatgagcca tcgaaaggcg atccacaaag gcaagaactt gatcagggaa
    29221 gatagtacga aattccgact caaggatccg attcagagga tcacgcgcgg atagttcaga
    29281 gaaatcatgc cggagtcgat cacgggtgct gtacgatcgc atgtgtgagg caatttcttc
    29341 gctgtagagc gcgtcctttt cctcatcact aaataccagc aatttgtatc gccatcccca
    29401 gtcctgggga tcggccatcc gcgcaagaaa atccacctgc tgttcgaaag gccgaataag
    29461 tgccgaattc ccagtctgca ggtaggtctg aaaatttgcc aacggaacgg ctagacgatg
    29521 agagagatag cttccgaaga gttcatcggc cccgtcaccc gagagtgcca ccttgacgtg
    29581 ttggctcatg agttgagcaa gaaagtacgt cgaaaccact cccgcatagg gctcatcaaa
    29641 acatgtcaga atgcggcgga ggttttcggg aaaactggac acttggatcg tttcctcgtg
    29701 atgttctgtt ccatattgtt ccgcgaccca acgagcccaa cggcgatcct cttctttccc
    29761 ttcggtcgtc gattcccgcg aatacgtcag cgtgaaactt ttgatcttgg aaggcgagag
    29821 ttccgcagca agcaccgtcg acagactgga atcaatgcca ccactcagaa agaacccgat
    29881 cggtacatcc gacaacagac gccgctcaac cccctgccga agcaggacaa gaagccggtc
    29941 agtaatctcc tcctccgtca ttcccgcgct cgtgtcgctt cccgcgaagc tcagatccca
    30001 gtagcggctg acaactggtt cctgtcccgc gcgatacgtc agcatatgcg cagggggaag
    30061 catggcaatg ccctcgaata tcgaaagagg atggggcacg tgcttcaaac tgagaaattg
    30121 gtgcagcgca tcaagattca agcgccgagt gaagcccggg atttgcaata aggacttgat
    30181 ctccgacgcg aagtagagcg cctgatcgtg cagacaatag tagaggggct tctttcctgt
    30241 tcgatcgcgg gccagatagc cgacttggcc gtcaccgtcc cacaaggcaa cggcaaacat
    30301 accgtctatt ttctttggga atgccgcccc gtgctcctcg tacagatgag gcaagacttc
    30361 agtatcgcag tgggtcttga gcacatgtgc acgctcgagc aactcatttc gaagtgcagg
    30421 aaagttgtaa atctctccat tttgggccgc ccagattgtc tcccgctcat ttgagattgg
    30481 ctgccgccct ccctgaagat caagaatgct aagccgtctg gctccgatgg cgaagtttct
    30541 ccccgacacc acatgttcat catctgggcc acgatgggcg aggagcgcga ccatcttttt
    30601 gacagcctga tttgggcctg gaccaaaccg tcccgcaatt ccacacatat cctttaacgc
    30661 ccccctgccg gagcataggt ccctggccgc accccttcat gaaccggtga gaccgcatgc
    30721 ggcgcccgca cagataccga aggatcgatc acgcgttcca cgactgtcat ggccgcggta
    30781 tccaccgtat attccgcaac cggcgcaaac acaaatcggt ccggttcttt tcgtagactg
    30841 agatactcaa aaatcaacaa ctcacccttc tcgcagggaa atgttcgtgc atatcgaagc
    30901 agacgttcgt ggaacaatcc gctattgtga ggataatact tcagtgtcgt aaagatacag
    30961 gcgagcacaa tcgactctgc aacgtggcca aaccgtagtc gtgcccctac actttcgtcg
    31021 gaaataagtt gtcgatccga ttcatgcgct ttcggatagt gataaatccg aatgaaaaaa
    31081 ttgacgatat cgacagagaa tactctccaa acgatcaatc caaagaaatt cgtatagcgc
    31141 tccagggcca tctggagcgg ttttggcagt gagcgccgca ggtaaaagtt atacgagagg
    31201 cccacgtgtg caaacggcag caggatgagg tagatggcta gtgcccaacc caggaccgta
    31261 ttggccacag acgagaacgc aaagcttgcg acagagggag actggacctg tccgaccaca
    31321 gccgtaacaa cgatatcccc gagactgcca ggatgaagaa aaatgagact gctgcagaac
    31381 atgacaattt cacacaagag ccccagcctg atctgagtgg cgatgaacgc aaacatcccg
    31441 aatatgatca tgcccccgat gaatcgagtt gggggtaaga gcatgagaat gcctgcgata
    31501 atttgtgaag accaggcaag gtgattgagg aaccggaaga ggggatggga gggggagaag
    31561 tgctggtagt gcttccacca gtatccccag gcaggattgg tcatgccgag ttccattcca
    31621 tggttcttcg cataaccggc agagagtttg tagactccgg aggagatgta cacacacgca
    31681 aaatccgcct gcaaaacttg taatgcgaga gaccgcagct ctggcgcata atggagggta
    31741 tattccagaa ctgtgatcgc caatcccccc cagtagagaa tgtagccggg agcacccatt
    31801 ccccgcagta accccttcca gcgcatccct acaaagaaat acctgcagag cacgagattg
    31861 agtagagctg gccatggcgt ccaccagcca ccggtgatac aaacgccgca ggcaaaccac
    31921 accgccatta cgagcggaaa cacaagcggg ttctgtatga actcaacctc tcgtagtgac
    31981 tgagcatacc ctccccagcg ctcgctcaag aaaaagcgcc gccagttagg gatcgccaac
    32041 aacaacatgc cgcacagcaa catcccatac acgacacgca tgaacatctg cacggagagg
    32101 ctcagctccg ggtgcaaaaa accctgtggc gcgaaaaaca tctccatata gcggtcaacc
    32161 gtttcctacc ggaaggtata gcccaactga actcacgatg ttatgcgcac cacgcagact
    32221 aatgaggcac cacatcaacg tgatccgagg aaggatcttt gggaaggcga gctggcagcg
    32281 tacaagcatc ggactgacac ccaaacttca gtcgattttt cgccacccac gcatagaggc
    32341 gagttcccac cggggtagcg ccaggaaaat agaaaaccgg gatcaacggc cacaataacg
    32401 gactactcca tatcatccgg cgaaacgcaa aaaatcccct atacacttcg cgtcgctcgg
    32461 tcacgacaaa catggcattg tcaaagtcgg ccccttgtaa ctcaggaaac aacgcagcga
    32521 ccgactgttc gtcatgggca tcataaaacc gaaagacctt gaaaatatcg gctgcgctga
    32581 acagcttgag gctacgaata caaaagcggc attgtccgtc atagatgaca tacacccgct
    32641 ccttcatact catcgcgatc gcgactcctt catcactcac gcgcccaaac aagtccccgc
    32701 ctgcatgatt cgtttcaggt ccaaaccggt catgcgttcc ccatccagct cagccgtctc
    32761 aataagcacg tcacgatctc gagtgcacac ccgagctccc tgcgcgtcaa cttgcacgac
    32821 aagccccggc accccactgg tctcttgtac aaggaggcga catttccata ttactaattt
    32881 tttcttgttc aagatagtaa aggccccggg ccacgggcga accagagccc gcacaaggtt
    32941 gcagacctcc gtcgctgatg catgccaatt gatcaagcca tcgtcgatcg tgcgaagccg
    33001 atgatacttg gcggtcgcct catcctgctt ttccctgggg gctcgtcctt gactgatggc
    33061 aggccaccat tccttcagca aggccatccc ggcctccttc aacttctccc gcaccgtggc
    33121 tgcatcgtca tctcgctcaa ttggaaagcg tctctgtgca atgaccggcc ccgtatcaat
    33181 tccttcatcc atatagtgca aagcgacacc tgtctccgat tctccattaa tgatggccca
    33241 ctgcatgaca tgccccccgc gatactcagg caacaggcca ccatgcacat tgaccgttcc
    33301 ctctcgtggc acctcaatca catcaggggg gaggatcatc gggtatgacc acaccacaat
    33361 gacatcagga tcaagccgct ttaagtcttg gacgaatggc gcaatcgtct tccttggtgg
    33421 ctgcacgagt actggaattc ccgccgtgca agcaaagtct ttgataaagt cgacatcgtc
    33481 gccggatcgg ttcgagggtg ccaccaccgc gattacctcc gccccagccg agagtaacgc
    33541 cgtaacagcg gcggcagcta acgcgccgta cccgaagaca atcacgcgcc cacggtgcgc
    33601 ctcagcctta ttctgaacga cgttcttctc agacattgac tcactcaacg tgccgggcct
    33661 caccctagac tttcggttga aagacctcaa cttctacgcc taagtccctt cgcatcactt
    33721 ccttcaaatg gtcccctgaa ttccggagga ccttctgcca cccatgatcc caatcacgaa
    33781 taccagcgaa ccaggccgaa cacccacggc aggggaaaca ttccgccccg cggccctcga
    33841 gatgcatcct gcgataccac gtgaacattt caccctgcca aatttcctta atgctggtgt
    33901 ccttcacgtt gggaaagagc tctgaggttt tgaaagaaat atcctgccca cagagagcaa
    33961 tccgccctaa ggtatccaca ttcaatcgct caaacggcca tacgcacggt tcttttttct
    34021 gtttcggaag gtccgcgtat aaatgccggt cgagagtttt cccaagctgg atcgtcgtat
    34081 tgtcatccca agaaagaaac tttctggtaa tgacctcatc cacgccgaca tccttgagcc
    34141 aaaactgaac agcatcatcc agtctgccct cgatcgcctc ttgccgaatg atggaggtga
    34201 ccactcgggt cgtcgccaca tgctgcttcc tcaaatccaa cgcggcgcga acattgccga
    34261 catgcttcgt ccaccattgc tccggatccc cagtcttccc acttcgcggt ggacgtaaga
    34321 ccgaataggt ttcggcatcc gccgcatcca tcgaaaattc aatgagatcg attccggctc
    34381 ggatcacgcg ttccaaccgc tcccgatgct tggcatttgg accaaacata ctgccgttgg
    34441 tattcaacca aaccctggcc cctttcgcct tggcatattc aatcatctcg accattttcg
    34501 ggtggagcat gggttcgcca ccaccggtac accgcatcca tgcgccataa gggccacact
    34561 catcagcaat tttctcccat agtttcaccg gaattaaatc gccgtcatgt tcgtggtaga
    34621 accggcgcaa ttctgaattg gtgtcggtat agggacagtt tgggcacccc atgttgcacg
    34681 ggtaaatgat ggagataatc accatcaagg gaaactcttc cgcgccaggc tttagcccat
    34741 atgttttctc gtctggaacg accaccagcg gcttgcttgt cacgatgtat acccccaatt
    34801 agcggcagtt ctagaaataa tgttctcaca gtcgcgaacg tcggcgacta tcgcacgaat
    34861 caacacccat agacacatct ggcttgaccc tgaattttgc aaatcctagc tgaatgcttg
    34921 aataacggaa cagcccgtgc gagcaccaaa ccaggaaagg tgaagagatt agtatggcct
    34981 actctctcat tctgccatca tagttctcaa tccgtttcta tactacaacc cggcagtcca
    35041 cattttcccc gccccaaccc tgtatgcact cagctgttaa tcagcaatcc tctttacgtg
    35101 accttgatac caacgatgcg catcttgcaa gccctctctg agcggaatcc gtggtgtcca
    35161 tcccaatgcc ttcatgcgac cgcaatccat gagctttcgt ggcatgccat cgggcttggt
    35221 cggatcccaa tggatatcgc ctcgatagcc gacgacctcg gccactaaag tggccaaccg
    35281 gccgatcgaa attccttcgc cggccccaac gttcacgatc tctgcatggg agtaccgctc
    35341 catcagaaac aggcaggcgt ccgcgcaatc atccgcatgc ataaactcgc gcaatggcgt
    35401 gccactcccc cataacgtgg gttccccgcc ctgttcccgc gcttcgttaa atcggcggag
    35461 caatgcggcc aacacatggg aatgctgcaa atcaaagtta tcgttcggcc cgtacagatt
    35521 cgtcggcaca gcgacaatga agtcgcagcc gtattgctgc cgataggcct ggcacatctt
    35581 gatccccgca attttggcca ctgcataccc ttcgttggtc ggctccagag gaccagttag
    35641 cagatagtcc tccttcattg gctgaggcga gtcacggggg tagatacagg atgaccccag
    35701 cagaagcagt ttcttcacgc catgaacata ggcttgatga acgacgttgg tttgaattgc
    35761 caggttgtcg taaatgaact ctgcaggcaa cgtgctgttg gcgaggattc caccgacctt
    35821 ggctgccgcg aggaacacat agtcaggctt gacctcttca aaaaatgcgg ccacccgcac
    35881 attgtcgcgc agatccaatt ccccactggc gcggacgatc agtcgctcgt acccacggct
    35941 tgtcagagcc cgcaggattg ctgacccgac cataccctgg tgtccagcca cgaaaatacg
    36001 tgcctgcttt tccatcgact acccctcagt gcgggctttg caaccgcagc cgctcgacta
    36061 ccgattaatt tttatgtcgt acatggaatc aggcacctac atcgctatcc tgtgaatcgg
    36121 caactcggta gattcctcgg tagattcccc ggcagaacga tttcgtgtgt agctgggcct
    36181 atgggaactt tgcctctaaa atacttccga tcagcttggg tatctggcga aaaattccca
    36241 cattgctgag tggacgaatg aggagacgta cccgcatcgc tcgccatata actgtgagca
    36301 atggtcttga cgctggacca ctgttgacca agccgatatt gaaaattttc tacgaacgat
    36361 ggcaacaatt caggtttgca gcaggaacac acccagagca cgagggaacc ttctcgctag
    36421 cagtatagag aaaagagcga ggttgcacaa gaaatgtgcc gggccaggaa ggcggccacc
    36481 taagctgatg agacggttca gagaagggcg cagcctatgt gcgggaggac attgaggaca
    36541 gggccggcag agctttcagg aatcgctgca acccgttctg ccaggacggc atgctgaacc
    36601 cgaggtgatg gagacggtct gaggacagca ccgaataggc cggtcgcttt gccggtcttg
    36661 gcatatcctc cgtagtgatt ggctggacgg gcacccggta gccagccagt ttaacaatct
    36721 ccgctgcaaa ttcatgccag gtacagtgac cttcgttcgt gacatgcaaa aaacctcgga
    36781 tcggatgcgt cacgagtcga tcgatcatcc gagcaagatc ttctgcaaag gtcgggcagc
    36841 cctcctgatc ggcaacgacc cgaagagaag gacgctcggc cgccagctga agaatcgtct
    36901 tcacgaaatt cttcccctgc aagccataca gccacgcggt ccgcaccacc aacgtattct
    36961 cgcagcaagc cagcgcccgc gcctccccgg cgcatttgga cgccccgtag gcgctgaccg
    37021 gattggtctg atcggtttca acgtagggac gtgtgccacg gccatcaaag acgtagtcgg
    37081 tcgagatata tatgagcctg gctcccacct gagcagccgc gcgtgcgacg cgttcggtcc
    37141 cgtcggcatt gatcgccatg gccaattgcg gattgcgttc ggcgccatcc acatccgtat
    37201 gcgcgccggc gtgtatcacc gcgtcgggta cggcatcgac aatcaggcga ccgcagtcct
    37261 catgcgtcag gtcaaacgtg ggaagatcca gtagggtcag ctgatgcccc tgcaacacct
    37321 gctgaagctc cttgcctaat tgtccctggg ctccggttat gacgatccgc acgcggtccc
    37381 cttggccagg cgctggccgt actgttgctg ataatatgcc ttgaactcgc cggacttgat
    37441 ctttcgccac caatcctcat gcgtccgata ccactcgacc gtctgctgta gtccttcctc
    37501 gaacggcact tgcggcgacc agcccagggc gcgcaaccgg ctgcaatcaa ttgaatagcg
    37561 gcggtcgtgt cctggccggt cctgaacaaa gcggatcagt gatcgcgact ttccgagtgc
    37621 cgccacgatc tgttcggcaa ccgtgatatt ttcgcgttcg ttcccgccgc cgacattgta
    37681 gacagttccc ggctggccat cttcgaacac gtgctggatc cccgcggcat gatcgtagac
    37741 cgacaaccaa tcccggcatt gtttcccatc gccatagagc gggagtggtt ctccgtcgat
    37801 ggcattggtg gcaaacaggg gaatgaattt ttctggatac tgattcggtc cgtaggtatt
    37861 gctgccacgg gtgacgacga caggaaatcg gtaagttgtc cagtagctca agacgagcag
    37921 atccccgccg gccttgctgg cagaataggg gctgcgcggt tccaaccggt ccccttccgt
    37981 tgaactcccc tgttcgacac tgccgtacac ttcatcggtg ctcacttgca gaaatcgctg
    38041 cacccctgcc tgccgcgcct cctccagcag aatgccggtc cccaccacat cggtgcgggc
    38101 aaaggccccc gggtcaagaa tcgatcgatc gacatgcgtt tctgccgcgc aattgatgat
    38161 gccctcgatc cggtgttcct gcagcgcggc atgcacagct ttctgatcac cgatatccgc
    38221 atgaatgaaa cgatactgag gatggtccac gagatccgtc aggttctcca gattcccgga
    38281 gtatttcaag gcatcaagat tcacgaccga atgacggccg ctctgcacta atcgacgcac
    38341 cagatgcgac ccgatgaacc cggcgccacc cgtcacgaga attcgcatca gaactccatc
    38401 ctattcgcac cggtctgcgc cacgagttgg ttggcggcaa gcagtgactc aattgtgccg
    38461 gcatccgtcc accagccgtc gagcacgttc caggtcaact cgccggaggc gatatacgca
    38521 ttgttgacgt cggtaatttc cagttcaccg cgccctgatg gtttcagcgt cctggtaatc
    38581 tcgaataccc gggcatcgta gaaatagatg ccggtcaccg cataagagga cttgggatga
    38641 gccggcttct cttcgatact cagaacctgg ttgccatcga ggtgaggcac accgaaacgc
    38701 tgcggatctt tcacttcctt gagcaaaatc tttgcgccct tcttctgggt cctgaacgcc
    38761 tccgcggcct tcgcgatgtt gccctgaatc aaattgtctc cgagcacgac gcagatcgga
    38821 ccatcgtcgg caaaatgttc cgccaggcgc aaggcatcgg cgatgcctcc ttcgccctgc
    38881 tgataggtat agctgagatg tttgagaccg aagtcccgcc cattgcccaa caacttcaaa
    38941 aaatcaccgg cactgtttcc gccggttacc agcatgattt ccgtgattcc ggcattcaca
    39001 agcgtctgga tgggataata gatcatcggc ttgttataga ccggcagcag atgtttgttc
    39061 gtcaccttgg tgagcgggag caggcgtgat ccgagccctc cggcaagcac gactcctttc
    39121 atccgtcacc ccctccgcaa caggatcttc agaacaccac agccgtcagc acgatctgcc
    39181 gcctgcgaac ctagccttcc ttcatgtcga tcgtaacgtc tcactccggt tcagactaga
    39241 aaattcgtcg atatcgtagg cgccggtctt accggccaac cgctcgttca tacaccgcga
    39301 ccgtttgttt cgctgtccgt tcccacacaa attcttttga gcgggcgaga gcacgctcac
    39361 gcaaggacag ctgcaattca atcgattcca aaaccctggc cagttcctgc gttaattggt
    39421 ccgtccggcg cggatcgatg aggaccgccg cctctccggc cacttccggt agcgatgacg
    39481 cattggagca gatcaccgga cagccacagc ccatcgcttc tagtaccggc agaccgaatc
    39541 cttcatagag tgagggaaac acgaataccg tggcctgctg gtaataggtc acgagctctg
    39601 catcggacag gaggccgatc agtttggcct gagcgccgag cccacgccga gcaatagcgg
    39661 cacgaatatc ctctcgtttg cacacatcgc cagccaggat cagatcgaac cggttgcgca
    39721 aatcggcggg aagcgtcgcg aaggcctcaa cgagcacgcc gacattcttg gtcgggtctg
    39781 atccggcgac gcagaagacg tatggcctgc cctcatgccg atgttcggcc ggccggaacc
    39841 gccccgcgtc aaggccaagc ggtgtcacac tcacctggtc cggtcgaagc ggcacatgcg
    39901 ccagcaaatc acgccgggaa tgctccgaaa ccgtgatcac ctggtcgatc gtctgccaac
    39961 gatcccctac aagccagtct tcgatacgac tgttgaggtt ggtgcgggga cggagttcag
    40021 gaaacagcag cggtttgaca tcatgaatgg tcgtcacgaa ctttccgcgc ttccaggcga
    40081 cgcaggaatc gtacggaaaa tgcagcacat cgtacggacc aaccatcagc ggcgcaacct
    40141 gctcccagaa tcgacggcga aacagcggaa accgcaccac gcgatatcgc atattcggcc
    40201 gcgccgtgaa aggatgttcc ggctgaggaa ggaggatgtc atagtgattc gtctgatcca
    40261 cgctccccag cgcctcgatc aattcccggg cataccgccc gatccccacg ttgaaatttt
    40321 tcaggtgcca ggcggcgatg acgatcttca caaggttgcg ctggatggac ccctggtgga
    40381 ggagatctgc gtgacgcgac gggatggaac catagcccac ccgtcaagtc aaggcaagcc
    40441 ggctccatca cactgggtgg cccaatggta ccgcggttga ctgggtttgc cgcctgtgtt
    40501 accggtgtga cggcacaagg aattttatcc gtggcacacg tcagcgtcgt catccccgca
    40561 tacaatggta cgtccaggta cttgaacgag gccatccgtt ccgttcaggc acaaagtttt
    40621 gcggactacg agatcatcgt cgtggatgac gcgtcgaccg atcgcacgga tgcgctggtt
    40681 caggctattc cacaggttcg atacgtacgc cactccaaga acgccggaca agccgccgcc
    40741 agaaataccg gcgcgcaact ggcgcaggct ccctatctgg cgtttctcga tcaggacgat
    40801 ctctgggaac cgacgttttt agaaaggacc ctggcgatcc ttgaaccggc cacaggcgcg
    40861 gtgctcgtcc attgcgacgg ataccaggtt aatgaacgaa acgatattct cgagtacgac
    40921 gcagcgatga aacatcagcg gagcattacg caattgcttc gcggcggcca cgatgccgcc
    40981 acatccggct cattgttccg caaatcctgg ttcgactcgg tgggaggcta cgatgcagcc
    41041 ctctcgatct gggaggacat cgatctggcc attcgactct atggaccaga acgcttcatc
    41101 cacctccctg aaccgatgta ccgtcatcgg ctgtatggcc acaatgcgtc gagagacatc
    41161 ccctctctgc gcgcactgga cggccgccgg cgatttcttg agaaacatgc cgccacctgc
    41221 cggcccggca caccggaagg cgaagccctg tcgaacgact gggccattta ttatgccgac
    41281 ctcggcaagc atcacctgcg ggcgcatgcc cggcaagagg cgcgacaggc atttcgattc
    41341 gccctgcgtc accaaccgct caatttcaaa acctggtccc gactcctcag atcgtacttt
    41401 gtgtagcctg attcaggctc gcatctttcc tcgcgtagac tttcagttca gtcccatatc
    41461 ccagatgtcc gacacaaaaa caaaacgggc ggatcagcga tttttcaagg agacgacggc
    41521 agggtattgc cctcccgaat cgttgctcac acactcggtc caggctagcc atgaagtaga
    41581 gcgaacccag gccggtgcgc atcgattcga tccggaatcc ggccgcttcc aagatccgcc
    41641 gcaacgtcgc cttttcataa tgaaagagat ggcggggcaa ctcccaagga aaccaccagc
    41701 gaccaaagat cttggcctcc agactgccgg cattcgggca gagaatgacg atccggccat
    41761 cctgatccaa aatgcgccag acctctttca ggagcggcag cggaccgtgc agatgttcca
    41821 gcacatgact gaacaggacc gtatcgaagc tccggtccgg ataggctatc gcctctaaat
    41881 cacccagacg tacccgatcc ccgaagcaag ccttcgcctg tgtgacggcc acggcgctcg
    41941 cgtccacccc tgatacctcc caaccctgct cctgcagcga ggcgagattt ccccctgccc
    42001 cgcagccgac atccaacaca cggccttgcc ccacccacgg caacaggcca cgcccgcgcc
    42061 agcagcgcca caagaactca ggccagagga acattcgccg tacccgttgc catgctcgcc
    42121 cctgctcctg tgccggatag ccataaaaat cctgggcgat ccatcgcctg attgttgccg
    42181 accaccgttt ggcagcacgg cggagatctc cggcctggcg gggcgccgca tcagggtaat
    42241 acgtatcggg ataatatctg ccgatttctt gccgcgtcgg acgaggattc aagaacaatt
    42301 gacggcaatc caggcagcgc accacggtga acacatcatc ggtgacgcgg tgggtgagat
    42361 cgtattggct gaacatcgtc gtggtcgacg tcccgccgca cacttcgcaa tcgatcgtct
    42421 ccatcacctg ttccacatga gggttgcctg ggcagttaag acgtccaggt gcgtagccag
    42481 cgcgtgtaac aacgtatccg gccgggagcc ttgccgattg cctttcgata ctggctccgc
    42541 gcctcagccg ggcgaccggc tcgttctgcg gccgcgcctt tcaacagata gtaattggcc
    42601 cattcccgat ccagtgcagc ccgctgcgca gtcgtcaacg gcgcaaaccc ttcgagacgc
    42661 atgagaaagc gatcccggct cgccagtacc cgctcccggt gcgtctgttc atcgaaggcc
    42721 atgtcccgat acccatgccc tcgctgcagt ttcgtcacca acacctgatc gatacactcg
    42781 aagctggtca cccggaagag cctgatcgtg agatcggaat cttccgatcc gtagagttcc
    42841 ggatccaagc gtccaacttg atccagcacg ctcttccgga tcaacagggt cgagggcaat
    42901 gccacatgtc ccccggcgaa cgcctgcacc atcggctcgc ggctcgaacg ggcgatgaca
    42961 cgttcttcca acgtcccgtc ttcgaagcgc acgtcaaaat ccgtgtggac caatcccacc
    43021 tccggacggg tgcgcatgat cgccagctgc gcggccagct ttcccggcag ccatacatcg
    43081 tcgcagtcca gcagggcgat gtactgcccg cgcgccgctt caataccaaa attgcgagac
    43141 cgggccggcc ccccgtggct ccgttgaaac caccggatgc cgtcactctc ctccaggcgc
    43201 ttcaggactt cttgtgtgcc atccgtcgat ccgtcatcca cgacgagaat ctccacgtca
    43261 ccatgcgctt gtgcccgcac actggccaca gccttggcaa caaatggcgc cccgttgaaa
    43321 acgggaatga cgacactgac cgactcattc atgtcacaac ccttgcaccc ttcagatcca
    43381 ggatccggcc cgccgcttcg aatacttcat cgaccgtgat ctggcgcatg caactatcgt
    43441 ctccgcgcaa gcagctggga tggtaacagg cccggcaatc cagacctttg tagatcacct
    43501 gcgtcaatcc gccacgcggc ccccatcgct cgggataggt gggaccgaac aaggccacca
    43561 ccggcgtgcc caccgtcgcg gcaatatgca tggcaccgcc gtcgttaccg acgaacaacg
    43621 cgcaccgctt caccagtgag gcatactgca gcagggaaaa ctttcccgct accacgatag
    43681 gagcgcaatg cgactttcgg gcgacctgtt cggcgacctc acgctcccgt tggtcaccac
    43741 ccagcaaaat ccggcaacga aactgttcaa cgaggcgatc gcttagctgc gcaaaccgtt
    43801 caggcggcca gactttcaac gaataccgcg cccccggttg caggagcacc agcggcgggc
    43861 attggtcacc gatcaatccc tgctccgtca gccattggtc cgtcacctgc tcttccgagt
    43921 cggacacaga gaccgacggc ataccccgtt tgggatcgag acccaacgct ctgagagcgc
    43981 acagatcata ctccacgcga tgctgatcga cggggcgcgg cttcgccacc ctgctgtaca
    44041 agagtccacg ccagcgatgt tccgcattaa atccgatccg gatgggtgcg ccggtcgcga
    44101 ggctgatgac ggcagatcgg tccccatcgg tcaggtcgat cacacaatcg aatccccgct
    44161 ctcggagacg atagacgaac ttgcattggt cggcccatgc gcccttttca agacagagca
    44221 cctcgttcac atccggatta tgcgcaagca ccccttccgt cccgcgattg accaacatcg
    44281 tcagctgcgc ggcgggaaaa gcctctctca aggcgcgcaa caccggcgtg gacaacagca
    44341 catccccgat atgccgcaat ttgatgatca ggatgttctt cgggcgcagc aggccagtag
    44401 ctatcatgtg cagagctcac gatagagagc actggtggaa actgccgcgc gctgccaggt
    44461 gaagagcctc gcccgttcgt atccgcgctg tcgcagattc tctctgagat gcgtatcgga
    44521 caggacggtc accatggcat gggccaattc ttccacattg ttcggatcaa ccagcaatgc
    44581 tgcgtcacca gccacctccg gtaacgatga ggtcgaagat gtcacggtag gcgcaccaca
    44641 ggccatggcc tccaacaccg gcatgccaaa tccctcataa tgggaaggga acacgaacaa
    44701 gtctgcacag gagtacaatc gacgcaagtc ttcacggtcg agacgtcccg tgcaacgaac
    44761 ctcctgctcc aagccgagcg actgcgccgt cgctgtataa ctgccctggg gatgctccgc
    44821 gtctccgacc agcacgatgg ctcgtccgcc cagctgtgag cggcacgcgc ccaccgcctg
    44881 taaaaatccc acgtggtttt ttctcggatc agccccaccg acgaagagaa tgaagccgga
    44941 cgagggaatg gaccatcgtg tccgtacctc cgctcgaatc tggtcgtcac acagtggcgt
    45001 gaagtcctcc gacacgccgt tataaatcac cctcacatgg tctcgtggaa tgccatacag
    45061 cgattcgatt tcgcgggccg aaaactcgga gacggtcaca accttgcgcg cccgcttcgc
    45121 cgtcgcaatc gcacggcggg atgaccccgc ctgcccgaat agtttgcgtg aatattccgg
    45181 atgccgggcc agccacaggt catggatggt gacaattcca ccgtaccggc ccgtcgtgtg
    45241 cattttaaaa ttggggccgt gatacaggtc aagacgatcc cagcggcctc gccaggacat
    45301 caaccaccgt ggcgtctcca tccactgcat gcgcgagcgt gatggccaac cgctgagttg
    45361 accggcttgc agcgatccgg ggcggagata accgacgtag tccgtcttct catccagttg
    45421 caacagcgcc ttcagcagat gatacgtatg ccagccaacg ccgcctttat cgcccaccaa
    45481 tgaggacgcg tcgatcccga tacgcatcag aggaacgaga cctcagtccc aggccggcga
    45541 tggggcaccg agaccatcat gcgtctcgcc tgacgttcaa ccgctcccac gccttggcat
    45601 atttcacgaa ggtgtggaga ccggcgaaca tcgccaccac cagcccatgc acgccgtcgc
    45661 gatatccacc gcgcgtgaac aacgttttta gaatagtgcc gaggtggtgc gtggcaatgg
    45721 tccagccgct gatatggcgg acacgcttca acttttcatc ggcgcccagc gtggtatacc
    45781 gcatcatctt gcggatgtgg tggttcacgg tgggcatgct gtaatgaatg aacggctccc
    45841 ggagacgctc ccgccggccg ctcagcgcga ggttttcgtg gaggcgcaca tcgtcatagc
    45901 ggccggccgt cttgcgaaac agccgcagtt gatagtccgg gtacaacccg ccgccttgaa
    45961 tccatttccc gtaaaacaca ttccggcggg ggatttcgta gcccccaata tcagcaggag
    46021 gtcgactgtc caccagtgct tggatttcct gccgcaaagc gtccggcact cgctcgtcgg
    46081 catccaccac caggatccag tccgcaccgg cttggtcgat cccaaagttc ttttgcggcc
    46141 cgtaccccgg ccagggacgc acaaacacct tcgaggtgta cccctttgcg agctcgacgg
    46201 ttcgatcccg gctctccgca tccaccacga tgagctcatc aacccaccgt agcgattgca
    46261 gacactccgc gatattgtgc tcctcgtcct tggtgatcac cactgccgca atcgtcatgc
    46321 cgtcccgtcc tgcttcacga tactgtgcct agccttgttt cgccggggca acccggttcg
    46381 gctgccgaac gccctgggat tgcagttccc agaaccgcgc atatttgatg aatgtgtaat
    46441 aggcgtagag gccggacaaa atgaggccgg ggacaccatc caggcatccc ttacgcccga
    46501 catacatctt gaggaaggta aaacacggat gcgacacgag ctgatgccac gaaaacgctc
    46561 ggccctgttc gaccatccgc ttagccatca aatcggaata gcgttcttgc ttggccaggt
    46621 aatgatcgat gtcccggaat ggatactgga gcgcggcggc cgtcatgtag cccagtcgtc
    46681 catccagatc gaaactttca tggacgagtt catcccgata ccgcagcttg ctcttccgga
    46741 acaattgcgg ttgacgatag tcgggatacc acccgcaatg tttgatccaa cgcccgagaa
    46801 aataattttt gcgcggcacg aaatacgcgt cggcttccgg cccggcgtcc aacagccgcc
    46861 tgatttcgtc gcgcagttcg ggagtggccc gttcgtccgt gtcgagactg aacacccagt
    46921 catgcgtggc atgcgccagg gcttcgttcc tcagacgtcc gaacccatgg aagtcatgct
    46981 ggtagacctt gtccgtgaac tcgcgactga tgcgttcggt gccgtcggtg ctatgagaat
    47041 ccaccacgat gatttcatcc gcccaggtaa tagattccag gcaggcccgc atgttgggtt
    47101 cgtcgttgta ggcaatgacg accgccgata ccggtatacg atccacggag aagggtcctt
    47161 tttacatcgc gtccacggtc agcttcggcc gcagactggt gatcacaatc ggcttccgaa
    47221 ttcccgccag gaccacagtc gattggaatc cagcgcgaca ttcgcgactt cgtataacgc
    47281 catcaattcc cggtcacggg acaccgacag ccgcctgccg cctgtttgtt catccggtac
    47341 cggttcgaac cgtaacgtgg cttcctgaaa ggtttccgtc gcgaacgaat acaacaacag
    47401 tccttctgcg ctcgcaagcc cgaccaggtt gtcgtacaca ctcgtcagat aggccacctg
    47461 accaggcagg caatcttgca ccagggcgca gctcacatca tgtcccgcaa atgagcccat
    47521 cgccggcaat cccccgttca atgacaacag ggtcggagcc acatccacct ggctggcgat
    47581 cgtatccacg actcgaggcc gataggtcgc aggggttcgt aaggattcgt ccatccacag
    47641 aaacaacggc gaggcaaaat gcccgacctt tcgctcgata tcggaccctc ccaccggttc
    47701 atgccggccg tgatcaccca gaatgacgac gatcgtattc cggagcaatc cttcgcggac
    47761 aagccgggtg aaggtccgct ccagttcgag atccgtgtat cgcaaggcgg cgacatactt
    47821 atcctgcacc tgctgttgaa gcaactgaac ctcaggatga ttcatggggg ccgcgaacgg
    47881 atgatgcgtc gacagcgtca gcgtggagag gaaaaacggc ttgccctggg actgccgcag
    47941 cttgatccgt tcataacaga ggtcgaacaa cgcaccatcg acaatgccca gtcctgcccg
    48001 ttccgtattc gcggggaagt ctccctcgtc cagcaattgt tgcaagccat tgcgggcgac
    48061 aaacgtctgc aaccggttca ggtcccgatg ctgcccgatc accatttccg tactgtaccc
    48121 ttgccgttgc aggagggacg gcaggcacag atagtcgtga gcgtatcgcg tcttcattgc
    48181 ggcagcgcct tgtcgcggaa aggtgctgca gagcgtcgcg aacagccccc tggaggtctg
    48241 cactccgttc gaaaaaaagt tctggaaata gacactgtcc tgtttcagcc gatcgagaaa
    48301 gggggtgccc ttcacctcgc cataggtctg ccccagatat cggcgatcca atccttcgag
    48361 aaataccaac aggatgttgg ccggcttgga cagtcgaacg ccatcggggg aaggcgaagt
    48421 cgtccgcacg aacggatacc gcggatccac aaatgtctcc ccgggaccca gcaactgttg
    48481 agcggtgcgc acggccaggt cataaggcat ggcctcggta ttcgcccgcc gttcgatcgg
    48541 attgcgggat accacggcaa tccgcaaagc ctcggcggca aacagcacgg ggttctgcgc
    48601 cagcgtgtaa tacaccatgc tgccgatcgg cgcgatgcga atgccgtacg gaccggaatg
    48661 gtgaaacccg gcgccgccgg ccacgagtgc caccaccaac aacgcattgg cttgaaaacc
    48721 tgagcccggc cgccagcggt ccagcgccgg caccaccgcg cgggaaaagc tccaccacca
    48781 caatccaatg cccgcggctt ggatggctag aaagaccgtc agtcgccagg cccatttccc
    48841 ggcagcctcc aactccgcgc tggtctgttg catggcctgc acgttcgtcc cctcagtcgt
    48901 cgccgcgggc gcgaggagat cgccgatata ctccaggaac acaaagtcca tatgttgatg
    48961 attgaagccg tagtacccca tatcgacaca caagagcacg aacaggagca atccggccag
    49021 cagacacccc acatggaagg ccgggcgaaa cgagagcgaa aaacgccgcg gtgattgacg
    49081 caagccggcc accccctgcc gcaacagcgc gtagagaccg gccccgaccc cgcccagcac
    49141 gatggcgaag gtggcggtga tgaaatctcc tcgcacaccg acgaggagtg ttttgagcag
    49201 caacgacgcc gtgggcgtct cctggtcgag cgcatcgcgc agcagaaaca cccgctccgc
    49261 catttggatg acgacgaaca gaatccacca gaacagcacg cttttgcgga tagacgctcc
    49321 cgagtcggcc catcgtagca tcatgacgcg atgaccttcc gatagacccg ctccaattca
    49381 tccaccatcc ggtcgatcga ataggactgc tccacggtat gacgcccgcg tttccccatt
    49441 gccgcacgca gattggcatc cttcaggaga cgaacgatgc gatcggccag cgcttcggga
    49501 tcgcgtggtg ggacgagaaa acccgactct ccgtcggcaa tcacatcggg aatcgaaccg
    49561 gtggttgtcg acacgaccgg gagaccgacc gcgagagcct gcatcaacac ttgtggaatc
    49621 gtatccccct cgaccgtcgg gatcacaaat aggttcaacg accggaagac gtcgaggaga
    49681 tcgtcacgga atcccaacat gcgcacatcg gctgccagcc cgaggcgttc gatatgagca
    49741 cgaatgttct gctcctccgg cccctccccc acaatgagga atgccgccgc cggatgttgt
    49801 ttcaacacct tggctgccgc gtcgaccagg tagcgatgac ctttatagtc ccgcaggtag
    49861 gaaatcatcc cgatcagcaa gtgccttggt ggaataccga gctcagcacg gatatcgtgc
    49921 gtgggttgag ccggagaaaa ctgttccaca tccaagccga tcgggaacgc cgccacacga
    49981 tccggcgaca agccgtctcg atgtatcagg tcctggcggt tccgttctcc gccggtgacg
    50041 atcaccatgt cgaacaggcg gcggtataac aggtgcgtgg tggcattgtt gttcagcggc
    50101 gctgagatat ggcgcgtgcg aatgaccttg gggcgaggac tgaggagccg cgtggagagg
    50161 gctgcgatcc agctgtcccg cgaactgttg acgtgaacga tgtcgggccg ctcacgcgtg
    50221 agcaactgac ggaacgcaag aatggacagc ggcaggcgcc attgccgcat cacgaacggc
    50281 tcgaccggga gcccacgctc ctgagccatc gccatgatgg gactgcgcgg ctccaacacc
    50341 aggcggatgt gatggcctcg cgccaccaat cgttcggcat gcaggaggac agccaactcc
    50401 tgaccgccga ccgccatgct ggattccgca atgagaatgt tcacgatgat cctgatgtat
    50461 ccaatcgccc caattgccgc tgcaccgcgc gcacgacttg ctccggtgtc acgccggtca
    50521 aacattcgag cgtggcggcg cgggagcact cacgacgaaa acaggggcgg cactcgaccg
    50581 ggttggacaa gacgacatgc ccctggccat aggggccggt cttcaccggg tcggtcggcc
    50641 cgaacagcgc caccaccggg gttcccaccg cggcggcaat atgcatcggc cctgaatcgt
    50701 tcgtcacgaa cacagctgcg cggcgcaaca aactgggcag caagcctaca ggtgtctgtc
    50761 ctgtcagatc aaccgctttc gtgcgcatgc gcgccatcac cgcacgcgtc tcggagcgct
    50821 cggccgggcc tcccataaag ataacgggta tgtgctgtgc ctcggccaaa agatccgccg
    50881 cttcggcaaa gtgttgcgcg ggccagcgct tggtctccca gcgggcggac acgttcatcg
    50941 cgatccatgg cgctgacacc ctgatacccg ctgcggtgag cagcgcttcc gcggcacccc
    51001 ggtcttcagc ccgatcagcg aagtcaaacc gcgggtccgt ctgacgcgcc gcacccaagg
    51061 cctccgccac caacagatac cggtcgacgg catgcatcga tccgttgggg acggcgacgc
    51121 gctgggtata caagaaggga ctcccttcgc gcgcattggc aaatcccaca cgccggctgc
    51181 agccggtcag ccaggccatg gcgccgcttc tgaacaatcc ttggagatcg acgaccagat
    51241 cgaaccgggc tgcccgaaga tccggcacgc gccccaacca ccctctcaac ccctgctcca
    51301 ccgcgcagac atgatcgact cctttgatga cctcgacgaa ggaagcccat tggcgcttga
    51361 ccagccacgt cacctcggcc tgaggaaatc gctcgcgcaa ggcggccagg gtcggcatcg
    51421 cgtgcacgat gtcgccaagc gaactcggtt tgatgagtag gatgcggcgg actggctcgg
    51481 tcattcggcc accagggatg atgctcgctc agcctgtcac atcggcaacc ggcttgcgcg
    51541 tcgcgtgggc aatggtccat tcaaccgcat gcgacaggtc agcggccaca tggtccggag
    51601 caagatcccg tgccgtcagg tccgccagcg cctcggcccc agaagggccg gtcatgacca
    51661 gcacagcccg cgcaccaacc tgtttggcca attcgacatc acgcacactg tcgccgacca
    51721 cataggcgcg gctcaggtcc acctgcaatt cggctaaggc gcgatcgacc atccctcgag
    51781 acggcttccg gcaattacag cgatcgtcgg gatgatgggg acagaagtac aacccgtcga
    51841 gcgtgacccc gtcttcagcc agcaggagtc gcaatttgtt atggatcgct tccagatcct
    51901 ttgagctgaa ataccctcgc cccactccgg attgattcgt cacgacgacc agctttgcgc
    51961 cgccgccttt cagtctggcc agtgccgcgc cgacaccggg aagcagcgta aattcttccg
    52021 gcgtcttcac ataccctgta tcgacattca acgtcccgtc gcgatccaaa aagacggtca
    52081 ctcccgccag cgccgtgggt gacaaggcag gtttcaccac tgtcggcaca atgagggagg
    52141 gaggggccgc gagcggcaga tgtcgtgctg ctgcatcgtg gacctgctcc accgtcactc
    52201 cggtcataca gcgatgatcg atgggacatt cccgcaacag gcatggtgcg caagataccg
    52261 gctgccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    52321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg aatcggcggg
    52381 gaatgccggc caggaagctg atcagcgccg cctcaaacgc attctgaaac agaatggcaa
    52441 gatcgaaccg atggcggcgc aagacaccgg ccaaggtcca cttgccggtc aatcccgcgt
    52501 gacgtccccg gtcgtcatag accaatgtgc gattcaccgc cggatgctgc gccagcagat
    52561 ccgcgatggc cggcttgacc agcagcgtga tctccgcctg aggaaacaac gtgcgcacct
    52621 gactgagggc gggctcgcac atgacagcgt cgcccagcca attcggaccg cgcacgacga
    52681 tgcgacggat ctcctcctta cgcatcgctc gccggcctac tttccggctc aaggcgcagg
    52741 agtcgctcca gccgttcctg tccggaaata atctgcgtgc gaagccggat ggcccaacag
    52801 gcgtcatcag cgttcaggaa gggcgccacc ttgtctgcgt ctttctccgt ggtcaccagc
    52861 agatcaacac cccccgcttg tgctctggca cgaatcgtct ccatcatggc atgggtatag
    52921 tgcacatgat cgggaaatgc cagtgtctca atcaccgtga tacccaactc cgccactaat
    52981 gcgtgaaaag acccggcatt gccgatcccg ctgaaaatga ctgccgagcg gccgcgaaag
    53041 gcatcccgcg gccggccttc atccccacct atgcgccgga attcctctgc gggaaacccc
    53101 acacagacgg gggacggaag cgccgggcat gcctgcacca gctgccgcca gacctgttca
    53161 cctgcctctt cacgatcgac cctggtgatg agcacagccg atgcacgggc cgctgctgaa
    53221 agaggttccc gcaagcgacc gaccggcaag gctgctctga gcccagccgt atcggtggcg
    53281 tccaccaaca agagatccac atcgcggtgg agttgcacat gttgaaatcc atcatcgagc
    53341 acaaaacaat ccaccggcaa acggctcagg acccactggc caagggcata ccggtctgcc
    53401 ccgaccgcca cgaccgcatg aggacaacgg cgggccatga gatagggttc atcaccggcc
    53461 tcttccggtc ccaccaacac ctgctgcccg tccgacacca gcaattgtgg agccttgctg
    53521 cgccgccggt agccgcggct caggatggcg acgcgtttgc cttgggcgat caaccggtcg
    53581 acgacgtaca tgacgacggg cgttttcccg gttccgccca tagtgaggtt tccgatgctg
    53641 atcacggggc gaggcaaccg atgtgtctga aaccacccgc gtgcatagcc ccacaggcga
    53701 agtctggccg ctacctcata cggatatccc acccatcgca accaggattg tcgcatcggc
    53761 ctcatgagga tcgtggatgc ggcaaggctc cgcgcgtagc gccttcggag cgggtcgatg
    53821 tggcggatgg tggaagaaag gtggcaatga tctccgcgct gcgctgaagc gcgccctggt
    53881 tgtccgcgac cacttgctgc gcagcttgtc ccatccgttt cagatcgtca gaattgccga
    53941 ggagctcccg caggctctgt gcaaggcctt gagcaccatc cacgacacgg ccgccccggg
    54001 cattcagcaa cagcgtcgcc acttcggcgc aatggtcggt atgtggtccg aacagcaccg
    54061 gttttcccca gacagccggt tccaacaggt tgtgcccgcc tatgggcacc agggtgccac
    54121 cgacaaacgc cacctcggcc tcccgataca acaacgctaa ttctccgcgc gtgtccaaga
    54181 ccaccacgcg cggaccggat gtggccatca attccatctt gccggtactg cgtcgagaca
    54241 ccggcagccc cttggcgcgc accatctgct cgacctgcgc tgcccgttcg atatgccgcg
    54301 gagccagcac caggcggagc tcagggaact cttcgcatac agactgatag gcatccacga
    54361 tcatgtcttc ctcaccggga tgcgtgcttc ccgccaccac cagccgctcg cgctcgctca
    54421 gtcccagggc ctgcttcgac acgaccgtgc cgccggcggc cgcctgtgga accggctggt
    54481 cgaatttgat attgccggtg caacggaccc gggattggtc ggcacccaac tcaaccatcc
    54541 gttgcgcatc acgctccgat tgcatgaggc aacagctgat catattcaac atcgtgcgat
    54601 aaaaatcccg gatcaggggc aagcgctgcc gctcaaacga tcgggtggag agccgtccgt
    54661 tgaccagaat cgagggaata ttccttcgcc gcaggctccg gaggagattg ggccagagct
    54721 cggtttcgac gaagacgtag agattgggac ggagcagatc gatcgtctgg ttgaccaccc
    54781 agggaaaatc gagcggagca taccggtgtt cggccagacc ttccaaacgt tgctcgacgg
    54841 cctcacgtcc cgtttccgtc acggttgaga ccaccaatct cgcctcggga taccgccggc
    54901 gaagctcccg taccagcggc gccaccgcca ccacttcccc caaagacacc gcatgaatcc
    54961 agatgcaggt tggcgccggc gcagcctcgc gcggctgctc cggcaacagg cccagccgct
    55021 ggggtagccc gcgccggcag cgctgcttcg ccagcaacac acagaggatg accggagaaa
    55081 cgaggaggag caggctgttg tagagcagat accacattac ggacgagcct gccgggccgc
    55141 cgcatcggcc tcgatcgtca tgcggttcag ggtcgcttcc aactccccgc gcaggcgctc
    55201 caggtcttca gcggatgcat ccggcgggac tgcaatcggt gccccgatga ggaagatccc
    55261 ctgcgagaat ggatagggca tgatgaaccg gtcccagctc gcgaagagtt tttttttgag
    55321 caaccgaagg ccatggggac aatgggtaga cctgtcgccc tcgccaactg caccacgccc
    55381 accttggcga cctgcctcgg ccccttcggg ccgtccggcg tcagcactag gttgccgccc
    55441 gaccgtccga ggcgaaccag ccggcgaaag gcttccgcgc cacctctggt actggaacca
    55501 cgcaccgcat ccaatccgaa acgcgccacg atccggcaga tcaattcacc gtcccggtgc
    55561 tggctgatca ggatatgcgc tttgagaccg ggaatggcca gcggcatcat cagctgctgc
    55621 gcatgccaaa acgcgagaat cgcctgctgg ccctgcgcga atatccggga cacatgttcc
    55681 ctgccctctg tcctccagcc catcgtgcgt tgcaggagcc gaatcacagc atagccgaca
    55741 ggcggcacca ccgcgagctt gagtgcattg gtgagacgct tcgtcagggc ctgtttcggc
    55801 tgcggggctg ccggagacct gttcgtcatg gagtcgctca ccacgtcacc ctcgatcctt
    55861 cgattactgc ggcagcgcat cggcaaactg catcgcatgc aggcgcttat acaggccgcc
    55921 tttgcgcagc aattcgtcat gcgtcccgat ttccgcgacc gtcccgcggt cgagcaccac
    55981 aatacgatcg gcattctgaa tggtcgacag ccggtgcgcg accaccaggg tcgttcggtt
    56041 ccgcatcagg ttggataggg ccatctgcac gatgcgctcc gattgcgtat ccaacgccga
    56101 cgttgcctca tccagcacca aaatgggagg gtcgcgcaga atcgccctgg caatcgccag
    56161 gcgctggcgc tcgccgccgg ataatttgag cccgcgctct ccgatcaacg tctcgtagcc
    56221 gtccggcatg cggctgataa agtcatgtgc gtaggccagc ttcgcggcct gcatgacgtc
    56281 gtcgatcgtg gccccctgcc gcccataccc gatgttattg cgcactgtat cgtcgaacag
    56341 caccacttcc tgcgacacga ttccaatttg tccacgcacc gattgcagtg tgcagttccg
    56401 gatgtccaca ccgtcgatca gaatctgccc gccactggga tcgtagaatc tcgggagcaa
    56461 actcaccaac gtggtctttc cactgccgct gctgcccacg agcgcaatga cctcgcccgc
    56521 ccgtatcgtc aaatcgatgg ctttgagggc cgcatccccc tgcccttcat actgaaaggc
    56581 gacctgccgg aattcgagcg aacgggaaat ttgcggcaac tccgtctgcc cgccattggc
    56641 cacttgttcc gtcggcagat ccagcacgtc aaagacgcgc tctgcagccg cgagggcctg
    56701 ctgcaccgtg ttgttcgcgc ctgacaatcg tcggatgggc gtgtaggcca tgaacatggc
    56761 ggccaggaag gaaaagaagg cgccgggagt catttcatcg tgaatcacca gatagccgcc
    56821 gtaccagatg atccccgcca ccccgagcac gccgatcact tccatatggg aggaacccag
    56881 ggaagaaacc tgaatcgctt tcattgtggt atggatgaac gcgtcgttgc tctgacgaaa
    56941 acgcttggcc tcgtcttcct cccggccgaa ggatttgacc atccggatac cggccagggc
    57001 ttcctgaaga gtcgacgcca tgtctcccat acgttcctgt ccgcgtgtgg cgagtcctcg
    57061 caactttttg cccatccgcg ccatcgtgac cacggacagc ggcaccacga tcatggacac
    57121 catggccaac ttccagttct gatagatgat caccccgatc atcgccagaa acgtcagccc
    57181 ctgctgaaac agatccttca acaccccggc gacggcattg gccatctgat tcacatcgtt
    57241 gatgacccgc gagaccagcc gtcctgacgt atttgtatcg tgaaagtgga caggcaagcg
    57301 aaccaatttg gagaagagtt gctctctgat gtcgccgatc acctgattgc cgacataatt
    57361 catcagatag ttttgaccgt agttgaacac gcctttgagc accgccacga gcaaaattgc
    57421 caccgggagg acgaggagca ggctttcatc tttgctgatg aacaggccat cgagcacggg
    57481 acgtaccagc caggcataag cggcggacaa gccggccacg agcagcgcgc acacgaatgc
    57541 ggcgcccaag cggaatcgat aggggttcag atatgcgagg aggcgcagat agtgtttcat
    57601 gatcggcact cagcgagcac ggcttcggca gctcgtcggg aggcccccgg cgtgcctaac
    57661 gcctgccgca cagacaacag ggcggccttc atctccgcat aggcgcgagg atccgccagc
    57721 agccgttcgg tctcttgagc caagcgttcc ggtgtagctt cgtcatgaat cagctcaggg
    57781 acgattcccc ggtctgcgac cagattggcc agcccgatcc agggcacccg gatcaacagg
    57841 cgcgccaatc gatacgtcac ccaggagggg gcacgataaa acaaaatcat cggcgttccc
    57901 accaccgctg cctgcaatgt agaggttccc gatttgacga cgagcagatc cgacgcagcc
    57961 atcacttcgc ttgcctggtt acgcaagact ttgatgggaa ccggactgtc cttcaagaga
    58021 tccgccaaaa actggtcctg caccgatgac gcttgcgcca gaataaattg catggtgggg
    58081 tggcgttgcg cgagccgctt caccgtctcg atcagcaggg ggacatgttc cagcaattcg
    58141 gctttgcggc tgccaggaaa gagcccgata accggccccg catccgacag ctcgaattgg
    58201 cggcgtaacg cctcccggtc gtacgttgag gccacttcgt cgagaatcgg attaccgaca
    58261 aaggtgcagc gcacgccggc cttcttatag agcggttcct caaacggcag aatcgcaagg
    58321 acatggtcga cccgctgctg aatccaccgc atccggccac gccgccaggc ccacacttgg
    58381 ggcgcaatgt agtagacaac cttcaggcgg caggctcggg cgacccgggc aaaatggaag
    58441 ttcagtccgg gattgtcgat caggaccacg agatcccaac gctcccgttt gatcaatgcg
    58501 cggatgcggg aaatcctctg cagcatggcc ttcaccgcag acagaccgat gagtcccatg
    58561 acatccaatt gaggaatatc cttcaccagc tccgcgccgg ccgcccgcat ggaggcaccc
    58621 ccgattccca cgatctggag cgacggagcc agctccttca atgccttgac caaatgggct
    58681 ccgtggagat cgccggaggc ctctccggtc acgattaaga tacgtggcat gtcgctctgc
    58741 tcaggcggac caacatcgac gtggcccccc tagagactgt catggccgaa tgccgccgca
    58801 gagacgtcgc cggcggaatg gcgctgcgtg aattgaccaa tggcgtccaa cacccggcca
    58861 gccaggttga gcgcggcctc gccgtcctca ccagacacga caggacgcga tcccgtggcc
    58921 acggcatgga tgaaggattc cagttccagc cgcaacggct cctcgtcccc ggcctggaac
    58981 gtttccacat cgatcgtcgg cctcggccca gtgccctgca cccgccgcga gaccatcgcc
    59041 tgacgggatt gaaaatcgat cgagacataa cggtcgcgtt gaaagaatcg caaccggcgc
    59101 atcttgttcg tggacacgcg gctcgccgtc agattcgcca cgcatccgct cgcaaacgcg
    59161 atacgggcat tggcgatatc gatattggag gagagcacgg gcacgccggc ggcgcggact
    59221 tcctccaccg gaccgggctg aaacgacagc acgagatcca gatcgtgaat catgagatcg
    59281 agcaccacat cgacgtcggt gccccgttcg ccgaacgcgc tcagccgatg gccttcgatg
    59341 aaggccggac gttcgataga cgcacgcatg cgctgcatga tcgggttgaa tcgctcacta
    59401 tgtcccacct gcagcaggca tccgcgccgg gcggccaatt cgacgagttc gtgggcctcg
    59461 gcggaggtca cggcaatcgg cttttccacc aggacatgtt tgccggcttc caggcaggcc
    59521 ttggccgccg cgaaatgggc agaggtcggg gctgcaacgc tgacgaggtc gacctgcgtg
    59581 agcacctcgc cgagattcgt ccaggcctgc acgccatgac gctcggcgat cagcttggcg
    59641 cggtccggcg acgcatcata cacaccggcc agcgtgacgc cggggagcgt tgcataatgc
    59701 ctcgcatggt gctgcccgag atggccgacg ccgatcaccc cggcacgaag cgccgtcatc
    59761 gtgccttctc ctgtctgacg aggccggctt ctgcatccat cgcgtatgcc ttcatgtcat
    59821 cccgacgata gcgatccctg cgcggttcgc cttcgcaatc gtgtcgtcgc gatccagcag
    59881 aatgccgcgt ccggcttcca gtgccagcac ggaggctttg acggcttcca tcacttcgat
    59941 cgtgcgcggg cccacggcgg gaagatcgaa gcggagatcc tgctgaggtt tgcagcgctt
    60001 gacgaccacc gctcccccct gtgccaattc accacctcga cgaatcgctc cgtccgtgcc
    60061 ctccacggct tcgacggcga cgatcacacg atccttcacc acgacgcact ggccgatatc
    60121 caacgcgccg atctgcttgc cgacatccca gccgtaacgg atgtcttccc attccttttt
    60181 actcggctcg cgccgtgtca acgtgcccgc ttcgacgaga atcccttgca ggccgaaggt
    60241 tgattcgcag attcgaatgc cttcctgttc gatttcctcg gcaacgcggc gaagaatgtc
    60301 gtcgtccttc agatgaatca atttggccgc cagcgccaac gcgcggaagt ccggacggac
    60361 cgtggtaaag acgtgcgttt tcttgatgcc gcccaacatg acggcctgct ggactccgtc
    60421 gcccttgagc gcgtcgatca acttgccgaa ttgcccgatc ttgatccaat ggatgttgtc
    60481 gacgtgctgg gccaattccg gatcggtttc cccttcatgc gccaccgcaa agacggagta
    60541 cccgaggcgt ttcgcattgt cggcaaagat gatcggaaac cggccgttgc ccgcgatcag
    60601 tccgatccgc tggccctcgg gcgcctgact gtcatgcgct gcacgtccgc ccacccttgt
    60661 tattcctctt cgccttcctg atctttgccc accgaccggc aaataccccg tttcgtccct
    60721 tgcatgaagt cggcgacttg cagaacatcc tggctcgcac cgaactgctc ccgcgccagt
    60781 ttgaccgctt ccgccacacg atgcccggag cgaaacagta cctcataagc cttcttcaac
    60841 gccgcaatcc gttcggccga gaatccatgc cggcgcagcc cgatcgagtt caatccatac
    60901 atgcgggcgc ggtagccacc ggccgctcgc atgaacggcg ggaggtcctg gccgagcgcg
    60961 caacaacccc caaccatggc gtaggcaccg atacgtacga attggtgaat gccggagagc
    61021 ccgccgataa tggcatggtc gccaatggaa atgtgcccgg ccagactcgc cgcattcgcc
    61081 agaatgagat gattaccgag atggcaatcg tgcgcgacat ggacataggc catcaagaaa
    61141 ttggaatggc ccacggtggt gacaccgccc ccctgaacag tagcccgatt gaccgtcaca
    61201 tactcccgca gaatgttatc gtgaccgatc acgaccttcg tcggctcgcc tttgtactgc
    61261 atgtgttgag gcggcccgcc caccgatacg aagggatgca gttcgcaccg ctcgccgata
    61321 tcggtccacc cgtcgatcga cacatgagac aacacacgcg taccggcacc gatcgtgacg
    61381 tgttcaccga cgacggaata cgccccgact tccacgtcat ctgcgagcac cgctttcgga
    61441 tggatcaccg cggtcggatg aatcttcact cacacctcct cgccctctgg caggccgccg
    61501 aaacaccggt cagcatgcgc ataatctcgc tgaacagcgt gtacacagcc tgtggttccc
    61561 tctactcccc accgaccggc ggggaaactg cgtctctacc ccgcggcctt ctcgccggcc
    61621 gccttttcgt ccatcaccat ggccgtgact tccgcttcgc agaccaactc ggtatcgaca
    61681 aaggctttgc cctgcatctt ccagaatgga gcacgtttct tgagcacgtc gacctccaat
    61741 cgcagaatat cgccgggcac gacaggcttg cggaacttcg ccccgtcgat tccggtcaaa
    61801 tacacgaccg gtcgccccac tgcaccgagc gatttgaacg caagcacgcc gccgacctgg
    61861 gctaacgctt cgaggatcag cacgcccggc atgaccggac gccccgggaa gtgcccctga
    61921 aaaaacggct cattgatcgt gacatttttg atacccacaa tccggcgatc aggatcgagt
    61981 tcgcgaatgc ggtcgaccaa caaaaacgga taccggtggg gaaggatcga ttgaatctca
    62041 acgttgtcca tcactgacat cagccccgcc tcctgtgttg cgtagacgat tacgatcgac
    62101 ggttacttgt tctgccgatc gaattccttg atgatctgtt ccgtcagatc gaccgacggg
    62161 tgactgtaga tcacgatctt caccgtcgac tcgctgccct tgtccaggac agccgtatag
    62221 ccttccttct gcgcgaccgc cgctgcagcg gcttgaatct tctgggcata ctccgccacc
    62281 atgccgcgct gcttttcctg cacctcgcga ttgaagtcct gaatgcggcg ctggtaggcc
    62341 tccagtttcg tcctgaagtg ctcttgcttt tccttgcgcg catcctccga catggaggcg
    62401 tcctgcatgc ccttttccag atccttcaat tcctggtcgt cggaatcgat gatcttctgg
    62461 cgcgtcgtgg aataactctt cagttcctct aaggcgcgtt tcccggcgac gctgcgctcg
    62521 atgacctgtt gctgatccat catggccacc ttgaggtgat cggcagccaa gaccggctgc
    62581 gccccccagg ccacaaggac ttgtagaaca ccggccgcca tccagttacg tacgttcatg
    62641 gattccctct catggatacg ttttgttgaa ctcctcgatc aactgcgacg acacatccac
    62701 cgcctcatca aaataaaggg tcggtcctcc gcgcccgcgg tcgaagatag cctgcagttc
    62761 caatcgcttg gctaccttgg ccgacaggga ttcgatctta tcccggaatc cgtccaagac
    62821 ttccttctgc ttgtcctgca cttcgcggtt cagatcagtg actttctgct gatattcagc
    62881 catccggcgc cggaactgct cttcccgttc acgtttggca gtcgcgctga gcacgctgcc
    62941 ttgcttgacg aagtcttctt ccatccggcg aagttctttt tcttcgagct cgatcagcgc
    63001 ctgccgattc ttggcgaagg aggccaacat gtccttcgct cgtttcccgg cgttggtttc
    63061 gcccagaata cgctgaggat ccaccacacc gatgcgtgtg gacgtcggta ccgtcgaggg
    63121 attcgagcgg catccgccga ccagcagcgt gacggccaga agcgccaggc agagtccatt
    63181 ccatccggct cctcgcacgc ttttccagcc taccagtcca cgcatgcgct caattccttt
    63241 cctacacccg cgccacagaa cctgcatcag aacaacgatc cgatcgtaaa ctcaaagacg
    63301 cccatacgct cggtcggccg gggatcgagg ttgattccgt acgccgcgcg caagggcccg
    63361 aacggagaaa tccacctggc ttcaaatccc gccgcactcc gcaacttatt aaactgcacc
    63421 ggctcgttgt cgtcgaatcc gtttccgtaa tcgaagaaga acaccccgtt caacttcgcc
    63481 tcggacgaaa tcgtaaagat aaagtcgttg ttaaaaatga gctccttggc ggaacccagg
    63541 agcgagcccg atggcgtcac gggacctgca cggccgaaca cgaatccgcg catcgtattg
    63601 atgcctccga cgaagaaccg ctcggtgagg ggaatgggcc ggcccccgat cccctctgtc
    63661 tgcccgtatc ggacatgtac cgagaaccgc gtgtcataga ccagcggggt gtacttgatg
    63721 acgtcgaaat acactttata gaaatggttc gatcctccga gcgcgggtgt tccgtagtcg
    63781 gcaccgacgc tcaagcgcca tccggtgcga ggatcgagat aatagtcacg cgtgtcacgg
    63841 aacaacgacg tacggaaccc ggtggacgat tgcgtcccga gctggcggca gatcagcgga
    63901 atcagatccg ggcacaaccc ttcttgcgga tcgctgtact tgagctgctc gccgaagaga
    63961 ctgatgcttc cattgacata ctccgacagc catcggctcc acgtggcgct ggctccggac
    64021 ttggactcga agtaggaaat atagttggtc atggaccggt aaatatcgag ctgaagggca
    64081 ttgtacgaat cgttcacgta gggattccgg aacgtcacga gtcccaagga ccgctgctga
    64141 cccaattgcc cgcggatacg acccaaccat ccgttcccac caaggttgcc ttccgtgatg
    64201 tccgcaatcg cgacgagttt atcgagagtg ctgaatccgc cgccgatgct gaactggccg
    64261 gtaggttttt ctttgacctt cacattgaga tcgaccttgt cgacatcaac ctgctgagga
    64321 agaatttcca ccgtttcgaa aaagttgagg ttgttcaatc gctgaaagct gcgtttcaag
    64381 gccagcgtat cgatgacgtc ctgctcatcc aggcgcagtt cccggcgtac gacattgtcg
    64441 cgcgttttgt cgttgccggt gacatggatc tcgcggatcc gcatcatctc gccctctttg
    64501 acgttgaggg tgatcgtcgc ggtgcgcgca ttgttgtccg gcgtcacgtt gggcacgaca
    64561 tcggcaaaga catacccctt cgcgccatag agatcggtaa tgcgcgtgat ttcgccgcgg
    64621 atcttggccc gttgaaagat ttcgcccggg cggattccta acccttcacg caattcatga
    64681 tcctcgaaga ccgtgttgcc gcggaacccg acttcctgca cgatgaacgg ctccccttcg
    64741 acgaccgaat agctgatttc gaaccacttc ttgtcgtcgc tcagttccag cgtcggctgg
    64801 ctgatctgca cgttcaagta gcctttgttc aaatagactt cgcggatacg ttcgatatcg
    64861 ttgcccaact cctcgcgctt caagacaccg gcatccgaca gcagcgacgg cagtttcagt
    64921 tgcgtcacca ggccgtacca gggaatccac tcgcgcgtgg ccgtcacttt aaagacttct
    64981 tctttggtca ccgcgcgcat gccttcgaaa ttgatccgtc ggactttcgc tttggtgcct
    65041 tccttgatga aataggtcag ccgtttgcgg tcctcatcga cggcttgaat caccggaatg
    65101 acctggcaat tgtagaagcc gtcttcctgg tacgcgaggc ggatcttctc tgcgctctct
    65161 ttcgcctgct gctggtcgag aaaggtttgg cttttgatcg tgattttttc cttgagcttg
    65221 tcgtcgctca attcctcgtt gccatcgaac acaatctcgg taatgaaggg tttctcacgg
    65281 acgatgaatc cgacggcgac gccgccatcg gccgtctccg tttccacctg cacatcctcg
    65341 aagtagccgg ttccgtagag aatcttgacc tgttcccgga gcgcgtcagg agtaaaccga
    65401 tcgttggcct tgagggtgag acgcccctgg atcgcctgca gttcaatgcg tttctgacca
    65461 cggatggtga cggaattgac cagcggggca ctgacttggg cccgcaccca ccctgcttcg
    65521 ccgatcacga acagcgccgc caggacaagg acgagaatcg gcacccaccg cctgcctccc
    65581 gacttggtca ccgtaatcct tccgtaccgt ctgggagagc aagcactggt tcgttcgacc
    65641 ttcaggatga gagatcgtca catgccatgc cgcgtgtctt gccgcgaacc gatggtcggg
    65701 cgaagggacg cgaactgtcg tcataagagt caccgatgcg ccgcgttcgt tgcagcacca
    65761 ccgcacgtct gcagaaaaac tctcggatta tattgagcac atttttcaat gtaaaggagt
    65821 ttccctgcgc ggcggcgcgc cggcagcgat cgaaaagagc cgacgaaatc ttgacttcat
    65881 gtaccccatc acatactatc gccgttatga caagaacaga cgtacgtaaa gcgattattc
    65941 ccgctgccgg actgggcaca cgcttccttc ccgccaccaa agcctccccg aaagagatgc
    66001 tgccgctggt cgacaaaccg ctgattcagt acgtcgtcga agaagcggtc gcgtcgggca
    66061 tcgaagacat catcgtgatc acggggcgag gcaagcgcgc gatcgaagat cacttcgatc
    66121 gttcccttga actcgaagaa aatctcaagg gcagcggcaa agggcaggtc ctcaaccaga
    66181 tgcggcacat ttccaatctg gcgaacttct gctacgtccg ccagccggaa gccatggggc
    66241 tgggtcatgc cgtcttgtgc gcgcagcatc tgatcggcga tgaaccgttc gccgtcatcc
    66301 tgggtgacga gatcatcgat gcggcggttc ccggtctcgc gcaattgatc catatctaca
    66361 agcagcgcaa aggcgccgtg ctcggcgtgc aggaagtgcc gcagcaggaa gtcagccggt
    66421 acggcatcat ctcggcccgc aaggtgcgca gcggtctgca ccgggtggaa gacctcgttg
    66481 aaaaacctgc gcccgccgat gccccgtcga atctggccgt catcggacgg tatgtgctgc
    66541 cacctgaaat tttcccgatc ctccgcaaga cccctccggg caagaacgga gaaatccaat
    66601 tgacggatgc cctgcggcaa ctcgtgaaga acacgccgat gttcgcgcac gaagtcgaag
    66661 gacagcggca tgatgccggc gacaaactcg gatttctcat cgccacggtg gaattggccc
    66721 tgaagaaccc gtccttggga cctgcattcg gcgattttct gcagcagcga ctccgaccga
    66781 ctaccgccga cgtccgtcgc cgcggcccgg gtcgagggcg cgcgacaacc tagccccggg
    66841 catgatggat caccgtcacc cgaaccgtgc cctcgcacga catcgtcact gagaccatac
    66901 accagcagac tggagcctca tggctgaagc agtagaacaa gatttttcga gcaatcagaa
    66961 cctcgaaccg cccgatcagc ttccactcct ccccgtccga gacatcgtcg tctttcccta
    67021 catggtgctc cccctgttcg tcggccgcga aatgtcgatc aaggcgatcg aggcggcgct
    67081 ggcggggaac cggatgctgt tcctggcgac acaaaaatcc ctggatgtgg aaaacccgca
    67141 accggaggac attcattcgg tgggaacagt gggcatcatc atgcggatgc tcaagctgcc
    67201 cgatgagcgg atcaagatcc tggtgcaagg cctcgccaag ggacggatcg aacaatatat
    67261 ccagaacgac ccctactact ccgtcaaaat tgaaaaactt gtcgagacga agcagcccgg
    67321 ctccaccttg gagaccgagg cggttatgcg caccgtcaag gagcagatcg aaaagatcgt
    67381 cagcctcggc aaggtcctga ttccggacgt gatggtcgtg atcgaaaacc tggaggatcc
    67441 gggacgcctc gccgatatgg tggcctccaa cctggggctg aaagtcgaca tcacccaggc
    67501 ggtgctggaa attgtggacc cgatccagcg gctgcggcag atcagcgaaa tcctcgcgaa
    67561 agaaatcgat gtcctctcga tgcagcagaa aattcaggcg caggccaaag gcgagatgga
    67621 caagacccag cgggaatatt tcctgcgcga gcagctgaaa gccatccaaa aagaactggg
    67681 cgaattggac gagcgggcgg aggaagtggc cgaattccgc aagcgcatca aagacgccaa
    67741 gatgcccgag aaggtgctca aggagactga aaagcagctc aagcggctgg agaaaatgca
    67801 tccggacacg gccgaatcgg ccacagtccg cacctatctg gaatggatgg tcgagctgcc
    67861 ctggaataaa aagagcaagg acaatctcga cctgaaggct gcgatgaagg tcctgaacga
    67921 ggaccactac gatctggaaa aggtgaagga gcgcatcgtc gagtatctgg ccgtgcgcaa
    67981 gctgaaagaa aaaatgaagg ggccgattct gtgcttcgtg ggccctcccg gggtcggcaa
    68041 aacatcgctg ggaaaatcga tcgcgcgggc gctgggccgt gaattcgtca gaatcagcct
    68101 gggtggagtg cgggacgaag ccgaaatccg cggccatcgc cggacctacg tcggcgcgct
    68161 gccgggacgg atcattcaag gcatgaaaca agcgggcacg aacaacccgg tcttcatgct
    68221 cgacgaagtc gataaggtcg gcatggattt tcgtggcgac ccctcggcgg cgctcctgga
    68281 agtgctggac cctgagcaga acagcacgtt caccgatcac tatctcggcg tgccgtttga
    68341 cttgacggaa gtcatgttcg tcacgacggc gaacctgatg gatccgattc ttcctgctct
    68401 ccgggatcgt atggaagtca tcgatattcc cggatacacg gaagaggaaa agctcgggat
    68461 tgcgcagaaa tacctcattc ctcgccagat gaacgagcat gggattaccg agaaacatat
    68521 ccgcgtttca gagacggcga ttcgccatgt catctcccat tacacgcgcg aggccggcgt
    68581 ccgcaatctg gagcgtgaag tggccaacct catgcggaag gtggccaaga aggtggccga
    68641 aggcaaggct gaatgccatg ccatcgatca gaccaacctg aacaagtatt tgggcgtggc
    68701 gaaattcatt cccgaggcgg aacttgagaa ggacgaaatc ggcgtcgcca ccggtctggc
    68761 ctggaccgag agcggcggag atgtcctcta catcgaagcg accgtcatga agggcaaggg
    68821 tcagctcacg ctgaccggcc atctcggcga tgtcatgaaa gagtcggccc aagctgcact
    68881 cagttacgtg cggtcgcgcg agaagacgct tggcatcagc cccgaccttt tcgcgaaaaa
    68941 cgacatccat atccacgtgc cggccggcgc gacaccgaaa gacggcccct ctgccggaat
    69001 cacgatggcc acggccatcg cgtccgccct ggcgcaaatc cccgtgcggc gcgatcttgc
    69061 catgaccgga gaaattacgc tccgcgggcg ggtgttgccg atcggaggct tgaaagaaaa
    69121 aatcctcgca gccaaacggg ccaaactttc caccgttgcc ctgcccaaac gcaacaagaa
    69181 ggacttggag gagatcccca aacatatcct gaaagggatt gaattgatct tcgtcgatac
    69241 ggtggacgat gtcatcaagg cagccctgcg gcgggctccc tcgcgtaaag ctacggctgg
    69301 ggcaccgaaa acgccgctga agaaaccctc gcccgtgacg gcctccaagc gaaaagcagc
    69361 cgcctcgcca cgccgcaggc gtgcgttgac agggaccacc cgctcactgt ctctgtaacc
    69421 cgcgttgcat gaggactgct gctgccagcc ctgaatatgt ggtacggcaa tcggcctgga
    69481 agaaggtgga gtaatgccgc caggccgaac ccgccgggcc agcaccgtgc agagtgagtt
    69541 cggcctgatc cgaaaactgc gggctcaggc tgcccaaccg gatccgcaag tcttcaaagg
    69601 gatcggcgac gacaccgcga tcctcaaaac atcgtcaact gaatggaccc tcatcaccac
    69661 ggatctcctg gcggaggggg tgcattttga tcctacgacc tcctccttcc gggatatcgg
    69721 ctaccgcgcc gccatcgcca atctcagcga tatcgccgca atgggcggca cgccacggtt
    69781 tatgctggtc tcaattgcga tcccgcgggc ctgctcgacg acgcagattc aacaactcta
    69841 ccgcggcatg acggaggccg atgctcccta tcgggtgcgc ctcgtcggcg gtgacacgtc
    69901 tgcctccaag acaggactgt ttctcaacat cacgctcacc ggtgtcgtga aaccacgcca
    69961 ggcgctacta cgtagcagcg cacgcatcgg cgatctgatt tatgtcaccg gaacactggg
    70021 agactcccgt gccggccttg atctcctgac atcgcggcgg cggccgaaac tgcgaccgac
    70081 agatgctcgg tttctgctgg gccggcatca ccgtccgtcg gctcgcatcg cggagggact
    70141 gtggctggtt cgacatgggc tcgcgggggc cgccatcgat ctctcagacg gcctgactgg
    70201 tgaccttcgt catatctgcg aggaaagcgg ggtcggtgcc gaaatcatcg ccggttcact
    70261 gccgctttcg cgagcctgtc gagcgtatgc gatggctcgc ggcctcgacc ctgttgagat
    70321 cgcgcttcag ggaggcgagg attatgaatt gctgtttacc gtccctcgcg gacaacagct
    70381 ccggtttgaa cgactggtta agaagaccgg atttcgtatg acctgtatcg gttcaatcac
    70441 gccgaaggcg gccggtctac gactgaggac ggacgcgggc gccacacgac ccctgccact
    70501 caccagctac gaacattttc gcaggccgac gtaacgagcc cgatacctga cgacttatgg
    70561 ccgacgtacg atcgctgttt cgccaaattc tgcatctcga tgaaacgccg catcggaccg
    70621 cgctggcctt tgccgtcggc gtcttcatcg gcttttcgcc ggcgtatggc ctgcatatga
    70681 tcatggtggg cctgtgcgcc tggctgttcg gctggaacgt cgtcgcgctg ctcgccggag
    70741 cattcctcaa taatccctgg accctcattc cgatcctcgg cgcgacgtac tggaccggcg
    70801 cgaccctgct cggcatgcga gaatttccta cgttcgactg gagcgacttg tcattcaccg
    70861 gcctgtacca tcaggtagcg ccacatgcct ggccgtttgt actcggggga atggtcctga
    70921 gcatcgccgg cagcctgctg gcgtacccca tcgcctatgt gctgctcaca agatatcgcc
    70981 gtgcgagagc ggttgaacgc gccacccatt tgcccccgcc ctcagggctg ggctaagatc
    71041 cttcgtcggt catgacccta tgaaactccc atcctccact ccaccggctc cctacgacgg
    71101 atcggccctc atcgccgatc cgattcacga atacgtgtcg ttcaccgtgc cctatgcgac
    71161 accagaccca tcagaacgaa ctgaaaaaga cctgattgat tctccctggg ttcaacggct
    71221 gcgttacatt tatcagctcc agagcgcccg ctgggtgtat ccatcggcgg agcatacgcg
    71281 gttcgtgcat tctttgggca ccatgcatgt ggccggacga ttcgcgcgac acctgtatcc
    71341 ctttttgcgc aacgccgtgc ccgaccttcc ctctgccgcc ttcgtcgaag aattcctgcg
    71401 catcaccgcg ctcgtccacg acatcggaca tggcccgttc tgccatttct tcgacgacaa
    71461 ctatctgcac ggcttcggcc tttcgcatga aaagctcgga cagatcatcg tccgggagca
    71521 tctcggaccg ctcatcaaaa agattcgacg tagcccgtcg gggccgttcg atcgcggcga
    71581 ggaactcaat ccggatcaca tcgcgcatgt catcctgaag gagaagggaa aggacaattc
    71641 aaagattccc cgctggatca atgtgctcca accggttatt tcaggaagtt ataccgccga
    71701 caatttggac tatgtgctgc gcgacgccta catgtgcggc gtggcggtcg gaccggtcga
    71761 cctcacgcga ttgattcatt acaccatcgt gaccgagaag ggcttcacga tccataaaac
    71821 cggcctgccg gccctgcaca tgtttttgaa cacgagaatg tacttatact cgaacgtgta
    71881 ttatcaccgg acgaccaggg ctatcgacat tcatctccgc gatatcttcg gcgataccat
    71941 gaagctgttg ttcccctcga atccagccaa acatatggac gagtacctga cgctcaccga
    72001 ctggtcgtta ctggaagacg tgcgacgatg gaaaaaagcc gggcagagca gcctgcgtcg
    72061 cttgcaccag gaatgggcgc atatcctcag ccgcgacgtg aaatggaaaa tggcgtacag
    72121 cacggtactg aaagaaaagg gcatcgagcg gggcatggac tttcccagcc acgagcaatt
    72181 tcagcagcag attcagaacg cgttgccggc gaagttacga gacctgccgt tccgcgttga
    72241 tatggccccc ctggacccac gaccggaccc gaaagatacg cgcgggattc ctctgctcgt
    72301 ttacgatccc ggcaccaaag gactctccac cgagccgtta gaggagtttc tcgaccttct
    72361 tccaacccgg ctggtccagt ttcgcatcta tgcgctcgat catgcccatg atgccgccct
    72421 ctccagagcc gccgcgaccg tcttgaacaa gaccccgtcc agcatcgaga cgaatttctg
    72481 acccgtcgtt ccttgctgcc gccgcccaat gcacacccgg caaaacggat tccggccctt
    72541 actctcacac ctaggaatcg cactagggca tatcgcatct attgaatttt acttctctta
    72601 ctcatactga ctagacaatt gatctatcgc tctctgtcta tctgagcgac ggcacggtgc
    72661 gccacaaagc aggagcccca tgaccagcaa tggtctctac gggctaatga ccgacaagct
    72721 gacgcctcgc attcgcgaac tgggcatagg cgcgttcatt gctgcattgt gcggcgccat
    72781 gtttgtgttg gactgcctca ccccacgggg cattcccggg tgggtgctgt atacccttcc
    72841 cattgttctc accacctgga cccctcatcc ctggagttct cacgccgtcg ccgtcggctg
    72901 cgcggggctc acgtatctgg cgtatgtgtt gtctgctgat gtgccgggag tgcctacctg
    72961 gctgccacag gtcagccgtt cattcagcat tgttctcttt cccctcatcg cctatgtcgt
    73021 ggatcgacaa aagcgcttga cctccgaact gctgaagtcc accacaatgg ctgcagagca
    73081 caaggccctg ttggaccgat cgcagttgct gaggaagact gctgaagagg tacaggacct
    73141 ctatgaccgt gcgccctgcg gctaccattc gctcgatgcg aatggtctca ttgttgctat
    73201 aaaccggacc gaagtggact ggttgggcta tacgaaatca gaactgattg gaaaaatccg
    73261 atttacggac gtgatgacgc cggcgggtgc cggcctgttc aaggagcgat ttccgcaatt
    73321 tatcaaggat ggctacgtca acgatttgga gtttgagctg attcgaaaaa acgggtcgac
    73381 attctccgtc ctgctcagtg ccacggccgt gaccgactct gacggacggt tcgtgtcaag
    73441 ccgctccacg ctgattgata ttacggaacg caaacgcgct gaccaggccc tgcgtctgag
    73501 tcatcagtct ctcgaggcct tggtcgaaga acgcacggct gcgcttcagg aagccaacag
    73561 ccgtttggaa caggagctgg cacaaagcaa acagatgcat caggcactga ttgcgagcga
    73621 aacccggttt cgcgaacttg tcgaatccct gccgctgccg atctggacct gcctccctga
    73681 cggcacctgc gactatctca gccctcgatg ggcaaattat acgggacgat ctgcagacga
    73741 gcccttcagc accggatgtc tccagcacgt tcatcctgaa gaccgcccga gaatcaccga
    73801 gcaatggcac cactccgtgg ctcatattca gcccctcgat acggaactgc gcttcaagcg
    73861 ggcggatggc atctaccgct ggttccatgc gcatgcgacg cccatccgag atcacgatgg
    73921 ccacctgcta aaatgggtgg ggacctacac cgacatccac gatcgcaaac aagcgcagga
    73981 aatccaggcc agactggctt ccatcgtgga atcatcatcc gatgccgttc tgagcaaggg
    74041 cttggatgga cggatcttgt cctggaacaa aagcgcggag ttgatgttcg gctatcctag
    74101 cgaagagatt atcggccagc cggtctcccg gattctcccg ccagagcgcc gggcggagga
    74161 agccatcctc atcgagcaga tcacagcggg aatccgcatc caacaattcg agagcgtgcg
    74221 tctccataaa gacggccacc ccatccaggt gtccttgacc gtgtcgccca tagtcgatgc
    74281 caacggagag gtgacggcgg tgtcgacgat cgcccgggat atcacggaac gcaaacgtgc
    74341 cgaggaagtc ttgagtcaac agcgccgact cattgaactg tcctatgacc cgatttttac
    74401 ctgggatttt gagcggggca tcctggattg gaatcgggga tgcgaacaat tgtacggcta
    74461 cacccgtgcg gaagcgatcg gacgaagcag ccacacactg ctgaaaagta cttatccatg
    74521 ccctttcgac accatttgtg ctgaattgga agcgaccgga gcctggaccg gcgagttaca
    74581 tcaccgcacc aaagacgggc gggaggtgct cgtggaaagc cgctggggat tgatgaaaac
    74641 agggggtcgc agcctggtcc tcgaaacgaa ccgggacatt accgaacggc gccaagccga
    74701 attgatgatc ctgaagaaaa ataaggatct cgagaccctg ctctatgtga cctcgcacga
    74761 tctgaaggaa ccgctgcgcg cgattgaaag tttttcggtt ctgctgcagg aacgctatgc
    74821 gagccggctt gatgagaaag gacaggattt tcttcgccgc atcgtacgcg ccacacagcg
    74881 gctcgaccaa ttgctcaccg acattttgga cctctcccgc gcccagcgca tggaaccacc
    74941 ggtcgacgag atcgacgcgc atcagctcgt ccaagaggtg ttacgacggc tggaaagccg
    75001 catcaaagag agcggggcca cgatcacggt gtgcacgccg ttgccgcggc tccgggtcaa
    75061 tacgacctgg gccgtccagg gcatctacaa tctggtggcg aatgccttga agtttgtcgc
    75121 tgacgggaga cctccggata tcgagattgc tccccatacc gagattgatg ctgaggggac
    75181 ggaatgttcc ggcctggtca tacgcgaccg cggaccgggg gttccgtccg accagcgtga
    75241 gcgcatcttt gagttgttcc ggcgagcggt gggacgggaa atcgaaggaa ccggagccgg
    75301 tctggccatc gtccggcaag tggcggagcg gcatggaggc cgcgcctggg tcttaccccg
    75361 tgagggagga ggatcggact tttttatcac attcgggatc aaccggcagg ttgtgaaaga
    75421 ggtaggacta tgacgatgag gccgttcgat attctgatcg ctgaagataa tgaagatgac
    75481 attctcctca tccgcgaggc gttcgaaacc gtcaacatga ccaaccgggt ggccgtcgtc
    75541 cgggatgggg ctgaggcgat ggcctacctc cggggtgaag gctcgttcag ccaatgtccg
    75601 ctccccggca tggtgctcct cgatatcaat atgccgaaaa agacggggct cgaggtgttg
    75661 gaggaaatca aatccgaccc gcgatttcgt gccatccccg tcgtcatcct gacggtcagc
    75721 gagcgggaag aggatattgt gcgttcgttc gagaaaggcg cctgctccta tattcgcaag
    75781 cccgtcacct tcacccgctt catcgcggtc gtcaaagaat tcgaactcta ttggacgctc
    75841 gtctccaagg ttccgacgct caagaggtaa cacatgcagg tgttgctgat cgaagacaat
    75901 gaagatgacg cggcattgat tcgcgaggca ctctgcgagc actcaccgca ggatgtcctg
    75961 ctcacctggg cggaccgtct ggaagccggc ctgctgaaac ttgccgaagg ctctccggac
    76021 gccgtgttgg tcgatctgtc gctaccggac agccagggaa tggacacgct tgtccgcgtg
    76081 cgggcccaag ctcatgaagc ggccgtgatt gtactgacgg gactcgacaa tgacctcgtg
    76141 gcggagaaat ctcttctgca tggggcgcag gattatttgg ttaaagggcg attaacgggt
    76201 gatgggctga aacgcgccat ccggtatgcc atggggcgac atcgcgtaga gcaggcgttg
    76261 cggaaaagcg aagagcgatt tcatctcacc tgtcttgcca ccagagacgg catctgggat
    76321 tgggacatta cgtccgggga gacctggttc aacgatgcct atcaaaccgt ctacggctca
    76381 ggggcgaagg cgcgggaagc gggacggatt gcgtgggcag accaaatcca tccggaggat
    76441 cgccaatcga ttcttgcggg cctcagccat acactggagt ccgacaaaaa cttgtggacg
    76501 gcggaatatc gttttcgcag agcagatggc acctatgcgt atgtcatcga ccatggctat
    76561 gtgctccgga actatgccgg caccccctac cggatgatcg gggcaaaaac cgacatttcc
    76621 gaccgccggc aggcagaaac catgcatgcc gttcaactca ccgtgggcct cgcgctggaa
    76681 gaatctgtgt cgttgaatga ggccgtgcca aaaattctgc gcgcgatgtg cgaacttcag
    76741 ggctggacat tgggtgcgct gtggcttgtc gatactcaac actcggttct acgctgcaat
    76801 gcactctggc atccctcagg gcaacagacc gagcacttcg cccaggccta tcgcaccttg
    76861 ttcctaagac aggacatggg aatggctgga cgggcatgga aaacggacgc cccggcattc
    76921 agtccggata ttgtgaatga ggccgacttc cccgcgcatg atgccgcgcg agaggcggac
    76981 ctccatggag cgatcgcctt tccgatccac accggcaaag agatcctggg catcatggag
    77041 ttttttacgg tcgaagtcct gcaccctgcc gtgtcccgca tggagcgaat tgccgagctg
    77101 ggcgcgatga tcagccaatt tctcaaccgg aaagatctgg agcgacagtt gcgccaggcg
    77161 cagaaaatgg aagccctggg acggatggcg ggaggcgtgg cacacgactt caacaacctc
    77221 ctcaccatca tcaatagttg ggccgaactg ttgatggacg aaccgggcct gacgtcccgg
    77281 gcacaacgag gcatggcgca aatcagagaa gccggcaaca aggctaccgg actgacgcgc
    77341 caattgctgg ccttcacacg ccatcaggtc gtcgaacggc agtccctgaa ccttaatgag
    77401 cgcgtcagcg atatcgtcga actcatgaaa cgggtcatcg gcgaagacat tcatctcgtc
    77461 ctgaccctgg acccgacgtt gggacgaatc aaagccgatc ctgggcagat cgaacaagtc
    77521 gtgatgaatc tggtcgtcaa tgcccgggat gccatgcccc acggcggccg gctggaattg
    77581 gaaaccgggg aagtgatgat ctcccattcc gatccattct ggcctgatcc tcttcctcct
    77641 gggccttatg taaccctgtc cgttcgtgat acggggtgtg gaatgagcgc cgaaacattg
    77701 gcccatgtct tcgaaccgtt ttttaccacg aaggaattgg gcaaggggac ggggctcgga
    77761 ctgtcgacgg tctacgggat tgttcgacaa agcagcggca ccgtggggat cacgagcgag
    77821 gtgggaaaag gcaccagctt cacgatttac ctgccgcgcc tggaggaaga caccggcgct
    77881 cctcgcgcgg tgacgcagcc caaggtcacg agcgaacagt cggaaaccat tctactggtg
    77941 gaagacaacg acatggtgag aggtctggcc cataccgtgc ttgttgccca gcactatcat
    78001 gtcctggccg cccgctccgg agaggaagcg ctggaaatgg tgcgccggca gggacgccac
    78061 attgcgctac tggtgacaga tatggtgatg ccgggcatgg gcggcgccca gctggctcac
    78121 gaactgcaaa ccctccaacc ggacatcaaa gtcatcctca cgtccggcta ttcagggcgc
    78181 gatgaaacac tcctcaaaac attcgatgcc caggtggcct ttttgccgaa accgtatacg
    78241 cccgagtcgc tgaccaaggc cgtggcctca gcgctggaat cacagactcg acaaggaagc
    78301 tgcagggata cgtcgtcgca ctcaacctga agggttatca gagccacgaa gtcgacgagg
    78361 ggcgtactgg agggacggac taccggccgt caccaccgac tgctgtggga gctaattctt
    78421 cctgagcggg gaccgctggc gccgccagta ccgggcggtc cgcggcggga ttctgcggaa
    78481 agggcaattc gaggagcgcg taggggttca cccgagcgcc gttgactttc atcccccagt
    78541 ggagatgcgg accggtcgcc cgccccgtgg cgcccacctt cccgatcact tgaccggcct
    78601 ttaccagatc cccttcgttg accagcgctt ccgacaagtg aaagtacatc gagtagagac
    78661 cgaggccatg atcgacaaag atgcctcgcc ccgaaaaaat atgatcgacg acgagccgca
    78721 caaccccgtc attgctggcc atgacgtcgg tccccatcgg ggcgccgata tcttctccgt
    78781 tgtgtggatt cctgggttgc ccattcataa tccggacact gccaaaaatg ccggtccgtt
    78841 taccgtgaac gggctcgata aaccccgcct gccagagtcg catggcagac tcttccgcta
    78901 aggcctttcg aacctgctct tgctcagcct tccagcgcgc ggtcgctttc tcatcgagat
    78961 cgactttatc tttcggcagt ttcagatgct cgacggcaaa cttctccttt acgacaagca
    79021 cctgatacga aaaatgtctg gtttgctcgc ctagttgcgc atcgatagtc agctcatgcg
    79081 gtcccggttc atcttgcaga tcgataccga gcaatccgat gtagcccgtg cctccggcgg
    79141 tgggattcgg gaacagggtt acagtgcgac cgaggaactt tcctttgacg ctggtggctt
    79201 ccttgagttc cgggacggtc accacgacca cctgaccctg cttgccgctg aattgcccgt
    79261 ctccgccgcg aggaacaggc cccgtgttcg ggaagagctc aacaggaaac acgctggcga
    79321 gcagcacgac cgagatgatg agaaagcgat cgaggaacga catggtgtgg gctcgattca
    79381 ctccaccaac ccgtctcatc ggtacaatcg cgcaccggag acctgcgaca ataattcttc
    79441 aacgcgccga ttgactgcgc tgaacgggtc catacagaga atcgtctctt cccaataccc
    79501 gttcagtttc agataggtcc aatcgaccgt atacgaccga ttcttggcgc gtgcaaaccg
    79561 gatgaagtca ccgcggactt tcgccctggt agtctgtggc gggaccgaca tggctcgttg
    79621 taccgcctct tcctccacga cccgcgctgc catgccacga ttctccatta aataatacag
    79681 cccacgtgtc cgtttcacgt catgaaattg caaatccagc aggaacactc tgggatcatc
    79741 ccacccgcaa tcctttttgt ccgtatacga ctggatcaga tgttttttca tcacccaatc
    79801 gacctcatgc acgagctgca tcgggtcgtc ttccagccga tccagcacgg agatccattt
    79861 gtccagcacg tcgtccagaa ccggatcgtg atcctgcgcg gccagaaact ctgtggctcg
    79921 cttgacatag acgcgctgaa tttcgatcgc ggtcatctgt cgcccgtcat ccagcttcac
    79981 cttcttgttc agcgtaggat cgcgagagat ttcgcggatg gctttgacgg gatcctcgag
    80041 ttccatgccc gtcacggaga aacccgattc gatcatcgat aagaccagcg tggcagtgcc
    80101 gaccttcaga taggtggcgt attccgacat gttggaatcg cccacgatga tgtggagccg
    80161 gcggtatttc tccgcatcgg catggggctc atcgcgggta ttgatgatac tgcgtgaaga
    80221 ggtcgtcgag gaggacgtct tttcgtggat atgctgcgcg cgttgagaaa tgaagtactg
    80281 cggcttgccg gaaaccttca acaccttgcc cgcgccagaa tagatctgcc gggtcacaaa
    80341 aaacgggatg agctgctcgg tcaccttcca aaaatccaca tcccggcgca tgagataatt
    80401 ttcatggcac ccatacgtat tcccgagcga gtcggtattg ttcttaaaga tatagatttc
    80461 gccggagagc ccctcttccc gtaaccgctc ctccgccgcc ggtaaacagg cttccagcaa
    80521 ccgctcgccg gccttatcgt gaatcaccag gtccagaata ttgtcgcatt caggcgtgga
    80581 gtattcggga tggcagccgg tatcctgata gaaacgcgcg ccgttgacca gaaacgcgtt
    80641 cgacggccaa ctgttcggaa tcagcccttc aaagatgtag cccaggactt tctccatcgg
    80701 aaggtagatc ttcccgttgg gagagaaaat cagaccatac tcgttttcaa ggccgaaaat
    80761 tcgttgttgc atggagatgg atgccctcgg tgcgactcct gtcaggatac accctgacgc
    80821 gggtggcttc aacagactcc ggtgagcatt cgtcgtccac gcgcgatcgg aaattaccgg
    80881 acaatgaccg gccataccgc aatggactat gttgagggat gaacgttagg caagcagatg
    80941 ggagatatcg gcgctggtga gccgtcggaa ctttcgcccc tgacggtttc tgtccagtac
    81001 cgcgacctcg aggttttcag aaggaatctt ctgagtggcg acctgctcaa gggcggagag
    81061 acagcgccgg aggccatctt tgagcgtcgg tggctgcggc gtcgcatgtt cgcgcaggta
    81121 ttgctgcacg gcttctgatc gcccgccgat gaccgcaaaa cttttctcat ccacgatgct
    81181 gccgtcgaac gagatgcgaa agatttcgtt ggcctggccg ttgcccccga cctgcaccac
    81241 taggatttcc acctcgagcg gcttcacgtc cgtgctgaag gccgtcccca ggctttgtga
    81301 ataggcgttg gaaagagtcc gggcactcac atcttcgcgg ctgtacatat agccggtcag
    81361 atcggcgtgc cgtatccctg ccttgcggag atgctcgaat tcgctatatt tgccggcccc
    81421 ggcaaatgcg atacgatcat acacttccga caccttgtgg agagacgaac tgggattgtc
    81481 ggccgccagc acaataccgt cgacatactc caaggcgata atcgaacgtc ccttggcgat
    81541 gccttttttg gcatactccg ccttatcctg catcatctgt tcaggcgaga cgtagtaggg
    81601 caacggcatt gattacgatc tccttcgttc tgcgatcatg gcttcatata gggcacgcac
    81661 acgtgattcc ggcacatcgg taataccggc atcgctgaca accttggccg ttggatagat
    81721 tccccggacg agatcaggcc cccccgttcc cacgtcttca tccgccgcgt tgtacaaggc
    81781 caacaggccc acacggagcg cagcctcttc atcaagcccc gaccggtaat gttcccgcat
    81841 ggtattgcgt gcatctttac cgccggagcc gatggaatgg tgatcggact cttcgtaccg
    81901 cccacccgtt atgtcatatt tgaagatacg accttctttt cgcttcacat cgtacccgac
    81961 atagagcggc atcacgacca acccctgaaa caccatcggg agattggcct tcaccatttg
    82021 tcccagcttg ttggccttgc cctcgcagga gagctggaca ccctccagct tttcgtaatg
    82081 ctccagctcg gtttgaaaaa gcttggccat ttcaatacag gggccggccg caccggcaat
    82141 agccatggcg gaatactcat cgatgcggaa cactttttca atccgccggt cggcaatctg
    82201 aaacccctcc gtcgcccgcc ggtcgccggc aataatcgcc ccatcccgat acttcaatgc
    82261 cagcaccgtc gttccgtgcg gcaccgggat ggcgcctgga cggccgaggt ctagaggaag
    82321 cgtagcgcgg ggcatcgcca gatcgtgtac gcctggtctt aattcggggt gatactgggt
    82381 gagaaaatcg aagaaactcg aaccgtcgtg gtgtggaaga aagggcatac gattcatgtg
    82441 atggtcgcag tcaggttgat agtttttcga gcagcgcagc tgctgtttcc gcatgggcga
    82501 agagctcacc cgtcagcgct tctgtaccgc gcagagggtc cattaacgga atccgcttga
    82561 tcttgttctg cccgacatca aagagcaccg acgtccagct taccccgtag acggcattcg
    82621 ggtatttctt gacgcactgg ccgcgaaaat aggctcgcgt tccgacggga ggatttgtct
    82681 ccgcgcggac aatctcatgc tccagcacaa cccgctcgat catgtgactg cgttccaggg
    82741 tgtaatacaa tccccggtcc ggacggacat catgatattg cagatccatc aaccgcacgc
    82801 gaggatcgtc ccatccgcag gacttgcggt ccatgtaaga ctcaatcaga tagcgcttcg
    82861 ccacccagtc caactcccgc accaacaaac gcggatcccg ttcgaggcga tcaagcacat
    82921 cctcccaacg caccaggatg tctttcgtga cctgattcag ttcgtggcag gcatagtaac
    82981 tctgcgccgc cttgaggtag gcccgctgca cggcaatcgc cgttgtcgcg cttccgtcgg
    83041 tcagcttgag gctctccttc acctgcgtat cccgggacac ttgcttgatg gcattgacag
    83101 ggtcggccag gcttatccgg ggaagatcgg ctccagcctc cagcagatcg aggactatcg
    83161 aaagggcacc gactttgaga tacgtagaca actccgccat attggcatcc ccgacaatca
    83221 catggagacg ccgatatttg gtcgaatcag aatgcggttc atctcgcgta ttgatgatcg
    83281 gtcgccggac catggtgttc agatcgacga gcgtttcgaa aaaatcggcc cgctgcgaga
    83341 tctgataatc gacggaaggg gtctgattct cggcgccgac cttgcctgag ccggcataaa
    83401 tggggcgtgt caccagaaac ggcgtgagga cctgcgtgat tcgatcgaag gatacggcgc
    83461 gcgagacgag atagtcttcg tgatagccgt agctgttgcc tttgccatcc gaattgttct
    83521 tatacagaac gaattgatcg cggccgcgcg cctccgtgat tccggccaga gcctgcgcaa
    83581 tgatacgttc cccgacccgc tcaaaggcca caacctccct ggcattggta cattcaggcg
    83641 tggaatattc cgggtgcgcg ccgtcgacat acaaccggcc accgttggcc aagaccttat
    83701 tgagctgtcg attgtagtcc ggccccggac gctcgcgttc cccatcgacc tcgaacccgc
    83761 gcgcatccag caaggggttt tcattttcgt agtcccacac ggcctggggc gcgggcaagt
    83821 ttggatagta cccgatcaca tgaatggagt tcgctacagg atccgccgca ttcggatccc
    83881 ggctggcaat accaaattct gtctccgtgc cgatgacacg ggacagattg tgcgaatgag
    83941 tgtcattcat gggatatcgc gcgcctacag gtagtggccc gtgctgacgg tttcgatctg
    84001 gcgtgcctcc gacggcccgc cgctgatggt ccgtagatgg acgatcttct cgcctttttt
    84061 cccggcgacc ttcgcccaat cgtccggatt cgtcgtgttc ggcaagtctt cgtgctcttt
    84121 gaactcttcg cgaatcgctc gaatcagatc atcggcccgc aagccgctcc cctcttgagc
    84181 gatggcgcgt ttgacggcaa atttcttggc tcgcgagacg atgccctcaa tgagggcgcc
    84241 gctggcaaag tccttgaagt agagcacttc cttttcgccg tttgcgtagg tgacctcaat
    84301 gaatttgttt tcgtcggtgg tggcatacat ggcatcgacg gtgagattca tcagggagtc
    84361 gacaagtgct cgggcatccc cgccatgccg tgccaggtct tcctcggcga agggaagatc
    84421 tgtcgacacg tatttggaga aaatatcccg ggccgccgtc gcgtcgggcc ggccaacctt
    84481 caccttcaca tccaacctgc ctgcacgcag caccgccgga tcgattaagt cctgccgatt
    84541 actggcgccg atgacgatga cattggtcag gcgttcgacg ccatcgatct cggacaaaaa
    84601 ctgcggcacg atggtcgact cgatgtccga cgaaataccg gtcccgcgtg tgcggaacaa
    84661 ggcgtccatt tcgtcaaaaa atacaatgac agggtgcccg tcatcagccc gttctttggc
    84721 cttcttaaac acttcgcgta cctgccgctc cgactcgccg acatatttat tcagcagctc
    84781 aggacccttc acgtgaagga aatagctgcg cagctgccgg tcttttaaat gccccagctt
    84841 tttcgcaatg gagctggcca cggctttcgc gatcagggtt ttcccgcagc ctggagggcc
    84901 gtagagcaac acccctttcg gtgcgctcaa cttatagttg gagaagatat gcgggtacag
    84961 gaaaggcagt tccactgcgt cgcgtacctg ttcgatttcc cgctgaagtc cgccgatgtg
    85021 ttcatagtcc acatccggga cctcttccag caccagttcc tcggcttccg acttcggcag
    85081 cttttcaatg acgcaacccg agcgcggatc atacaagagg tgatcgccca cgctgagccg
    85141 ctcactcagc aacggctcac cgagctcggc caccttctct tcgtcgaagt gcaaggtcac
    85201 cagcgcgcgg ttgccttcca ggacatcttt caaacgcacc acttcaccct gtacgtcaaa
    85261 accgcgcacc gcgatgacgt tgagggcttc attgagaatg acttcctgcc ctttacgcag
    85321 ttccttcgcc ttgatagacg gatgaaggct caccttcatc ttgcgaccgg aaacatacac
    85381 gtttccggtt ccatcggtat ccagactgga aaaaatgccg taggtcgagg gcggagccgt
    85441 gagcttctcc acttcggccc gcaaggtttc gatttgtgat ttggcttctt ggagggtggc
    85501 gacgagcttt tcgttttgct tcgtcgtttg ctcgagttgg tagcgtgact ggtagagccg
    85561 ccgaatctcc tcctccatcg actggatttg ggtgcgaagt ttttccactt cgcgggcatg
    85621 ttcgtcgttc ttgtgcgtca cggctccatc tccgtcagaa agtgaccgag tcaggcgctt
    85681 gacggaatca cggaaggacc ggagtcggcc tgaactccct tttgaatcgg ccatcgcccc
    85741 actcgccgga ttgatgaaaa tcctaatcca tgacgatcat cagcactgtt tcggattcta
    85801 tcatccgcct atagggtggt caactgcacc aggagcggcg gcgacgccac gaccgtatgc
    85861 gtgttcaaca tcacgcgtgc gtgtgcggca tcacgactcc atctggatcg cagcaagcaa
    85921 ctcttcggtc gcctgctcga cgttcaccgc gcttaagatc gatgacaaca cggctacgcc
    85981 gaatgccccc gcttgtcgca cctcgcgcgt gcgagcagct gtgatgccgc cgatggcaaa
    86041 gataggaata cgtaccttct gcgcagcctg ttcgagaatt cgcatgccaa gcggcggccc
    86101 gtatacgcgt ttcgacggag tgtcatagat tggccccaac acaacgaagt ctgctccttg
    86161 ctgttcagcc gcgaccacac cgtcgaggga atgcaccgaa gtccccagca gttgcgaagg
    86221 ccccagcaga catcgcgcca ctcccgccgg caaactgctc tcgcgcaaat gtacacccac
    86281 ggtacgcagg gccagagcca catcgacgcg atcattgatg aacaaacggg catccggcaa
    86341 ctcccgctga agttccgttg ccagtgcatg caggtcgcga acaggcagat cgcgctctcg
    86401 cagttgaatt gctcgcaccc ccgcacgaac ggcgcgtgcc aggaccgatc cgagcggacg
    86461 cccggcggtt tgatgacgat ctgtgacgag gtacagtcgg aaatcgacag cagacatcag
    86521 gaggacatcg tgaatgcaga gtgatgaacg ctgacgtgac gcatcaactc acacgtggaa
    86581 ggatcgactt ccagctcgca acgtgtcacg cacaacttag aaatgtttac agcatgccct
    86641 cgatggggct gctggccgtc gcgtagagtt tgcgcggaat acggccggcc ttgtacgcca
    86701 atcgaccggc ccacacgcca tatttcatcg cttccgccat cgcgatcgga tcctgagcgc
    86761 cggcaatggc ggtattcatc aggaccgcgt cagcgccata ctccatggca agtgccgcat
    86821 ccgaagccgt tcccaccccg gcatcaacga tgatcgggac tttgaccgtt tccatgatga
    86881 ttttcaggtt gtatggattt cgaatgccga gaccagagcc aatcggggcg gccagcggca
    86941 tcaccgcagg gcatccaatg tccaccagtt tcttggcaac gatcggatca tcgttcgtat
    87001 agggcaacac gatgaatcct tccttgatga gaatcttcgc cgcttcgatc aatcctgcgg
    87061 tatcgggaaa gagggtcttt tcgtcgccga tcacttccag cttgaccaga tcagagacgc
    87121 cggccgctcg tgccaatcga gcatatcgca cggcatcttc caccgtgtaa caaccggccg
    87181 tattcggcag gatgatatat ttcttcggat ccagataatc gaggaggttt tccttcgaac
    87241 gatccgtgat attcacgcgg cgcaccgcga ccgtcaccac atcggcgccg gaagcatcga
    87301 tcgctttttt agtctcggca aaatctttat atttccccgt cccgacccac agacgcgact
    87361 gaaattccct gccggcgatc accaaccgat cattcatcgt atccatccct attccgctcc
    87421 gccgccaatg aagctcaata tttccactcg gtccccttcg cagagaccgc gagtttcgaa
    87481 atccttgcgg tccaggatct ccaggttcaa ttcgacagcc actcggtcag cgcgaatgtc
    87541 caagtcgcgc agcaacgccg cgaccgtatg tccgtcaccg atgccgcgag attccccatt
    87601 cacctggatc gtcacaaccg ctcctgaatg tgtcgaatca tcctatgaaa cgggaaaacc
    87661 ggttgtcaac caagggcgat aggccttgcc gtttgacagg cattccctcc acaatatggc
    87721 catcacgctc gataccactc ttcacgatgg atgaccgtct caatcaactc gcccagattt
    87781 ttcgggccat ggcgaaccta ctggcagccc aacgggccaa cccctaccgg gtgcgcgcct
    87841 accgtaaagc ggccgacgcc atcctgatga tcaccggaga cctgacagat ttggcggctc
    87901 gtcgtcagtt gcaggagatt cccggaattg gcaaggacct ggcaggaaaa atcgaagagt
    87961 ttctcaccac gggaacgatt cgcgcctatg aagaactcag aactccgtta ccgaaggaag
    88021 tcgctgaatg ggcctcattg ccgggcctgt ctgacgcgct ggtgagctat ctctatttcc
    88081 ggctgaatat tcgctcgtta gcggatttgg aatccctggt tgcgtcccac ctcttgagga
    88141 cgcaaccagg attttcggga tcggaggatg acctgctggc ggccatccgc cggcagcgtg
    88201 cctctgacgc tgttacgccg ccggtcgaag ctccttgaag attttccagg tcttcagcaa
    88261 ttcaaccgcc ttttgcaact gaacatcatc ttccacggag atatccccgc ctgcatcgtt
    88321 caatgaggcc gcgccatttt tatgcgccgg ttcatccgct ggcttggaag aagccgagtc
    88381 tttcccggag gccggagcct tcgccgtctt cggctcagct tctttcgcat ccttgtcgcc
    88441 cgctttcgcg acagtctgcg tctgcagttt cacgacgata tccggggtga tcccggtcga
    88501 ttgaatcgag cgccccttgg gggtgtaata cttggccgtg gtcaggcgga gccctgagcc
    88561 atcgccgagc ggcagaatgg tctgcacgga ccctttgccg aacgaggtgg ttccgacgat
    88621 caccgcccgt ccccaatcct gcaaagcccc ggcaacaatc tcggatgcgc tggcagagcc
    88681 ttcattgacg aggatgatca tcggggaatc ctccagcgtc tccttggtct tggagaacca
    88741 ctcgtccttt ttcccctcgc gtccctttgt atacacgatg agttttccat ttccgacaaa
    88801 ctgctccgat acatcgaccg ccgccgtcag caatccaccg gggttgttcc gcaagtcgag
    88861 gatggtcccc tggacctttt gctctttgaa ttgcttcaat gcccgagcga gatctcttcc
    88921 tgtggcttcc tggaactggg tcaatcgcac gtatccgatc gtgttatcga gcaccttgaa
    88981 cttgacgctt tcaatcttga tggtatcgcg aaccaacgaa aaggcgagcg gatccgcggt
    89041 gccgtcccgc tgaatcgtta ggttcacctt ggtgcccttc ggcccccgca tcttctgcac
    89101 cgcatccatc aacgtcaggt ctttggtggg ttcgtcgttc accttggtaa tgaaatcacc
    89161 ggccttgatc ccggctctgt gcgcgggagt tccctcgatc ggagatatca cggccaaccg
    89221 attttccttc acgccgatct gaatgccgac gccgccgaat tcacccttcg tctcgacttg
    89281 catctcctta tacatctcgg gcgtcatata cgcggagtgg gggtccagcg tcgacagcat
    89341 tccacgaatc gccccttgca cgaggtcttt caccttcgtg tcgtcaacat agtgtttttg
    89401 aacctgcgtc aacacttcgg aaaaggtgcg cagctcttca taggtttcag acgcgtggcc
    89461 ggtccgctcc aaaccctttt ccagcacgat cccgactccc agcgctactg tcaccatcag
    89521 caaaagatat aaccaccgcc ggctccgccg ctgttccata cgtgggttcc ttccttccga
    89581 ggcctaccgc tttgacagcc acaccagggg atccaccggt tccgccccct cgcgcagctc
    89641 aaaatacagc gtattttcac ctgtcatgcc ggtgtcgccc gtttcaccga tcgcattccc
    89701 ggcttcaatt cgggcgccca ccgacgtcaa gattttagat gcgtgggcgt agagtgaaaa
    89761 caccccgttg gcatgatcca tgattataac gagtccgtat cctttcaacc agtccgcata
    89821 gacaacctga ccggcaagca ccgatcgtat aatactccct tctgctgcgc gaatctcaat
    89881 cccttttcgt tgaacatacg tgttgaaggt gggatgcttc tggcggccaa agtaggagac
    89941 cacctgcccg tccgtcggcc acaagagcgt ccctcgtaat gcccgaagcc ctgctccggc
    90001 ggagggagga cgatttgcca aggcggcccg ccgccgggtc tcaagctctt tcaataaact
    90061 gtcgactcgg ctcgcggatc gctccagctc ctcgaccgcc cgatcatgcg actccttttc
    90121 ctgtgtgatt ttcgcaagat agacgcgttt ctgcttcttg agcccttgaa tttgagccag
    90181 cttttcttcc gtgctgatct tataaaggag aataccctgc ctggcctcct cacggctccg
    90241 ttccacttcg gcgatccgtt cggcgtcctt ccgatagccc tccacgatct catattcccg
    90301 ttgcgtcacg gcggagagat accgaaaccg gcgctgaaag tcgccggatg aactggcggc
    90361 gagaagggtc ttcagttgcc aaaaccttcc ctcgacatac tgaacccgca gcctggcggc
    90421 gatggcatcc tgccgttcat cgatccgctc ggacagacgg ctcagctgca cgctcatctg
    90481 ctcgatttcc acatcctttt ttttgagttt tctgacggta tcctggtgtt cttgccggta
    90541 acgcacgaga cgctcatcca gcgattgcag cccctgtagt atcgactcgc gctttttctc
    90601 ggcctcgtcg gccttcttac gcttctcgac aatggttccg cgtaatttct ccagcgtttt
    90661 cttttcctgc tcgatcttgt cggcatacga atccttgcgt tcggcatgtg caggcggcgg
    90721 gctcgtcgac aatgccaggt ggacgctcaa caccacccat acccactgaa tgcgcgtcat
    90781 gcccgcccct cgccaaaacg caacagcgag agaaaactcc ctgcgcatcc caggaacagc
    90841 cccaccagca tgagcaccag gcacatgtcg aacgaaaaaa acgtcaggag cgcatcgacc
    90901 cccagaaacc gcgtggcaga atgaatttga tgccggaaca attcgaatcc gccttttaag
    90961 atcaccagcg aaagggcgct gccacacaaa cccaatgcgg caccttccag caaatagggc
    91021 acacggataa acgtggtact cgccccgatc agacccagaa tctcaatttc ctctcgcctg
    91081 gagtacaggg ccagacgaat cgtgttggcg ataatggtca ccgaagcggc cgacagaatt
    91141 acgccgacaa tgatggccgc cacctcaatg taccggacaa tgccagccag ggcttccacc
    91201 cactcctggt tatactggac ctggctgaca ccgggaatca gttgggtccg attagcccat
    91261 cgccgcatgg cgtcggagga acgagactcc ggggccatca ggacaacgaa tgaggccggg
    91321 agcgggtttt gcccgagccc ctgcaggaga cgcgactccg acggaaattg ggcctgaaaa
    91381 tcagcgagcg cccgttcctt ggagacaaag gtcaacgagg cgattgctcg atcaatcttc
    91441 aattgttgtt ctatttcgct tcgcgcctgc tcagtcaggt catcctgcag atacaccatg
    91501 acctgaatat cctgctcgag agaagaggcc atggttctca aattcacgta gagcagcaaa
    91561 aataccccga cacaggcaaa ggtaaaggcg gtcgtcgcca ccgcgaccag ggtggtggtc
    91621 cgattggtca agacgttggc cacggcctct cgcaaaagat acagcaggcg tctcacgatg
    91681 ccccctgatc ccgggacacc acttttccgt gttccaaggt gatcacccgt cgattgacct
    91741 gcgccaaaac attcggatcg tgcgtcgcga caatgatggt tgtcccccgt gcattgatcg
    91801 acttgaacag ctcgatgatt tcgctcgtta actcagggtc cagattaccg gttggttcat
    91861 cggccagcag catgatcggc ccgttgacga tcgcgcgggc gatacaaatg cgctgttgct
    91921 cccccgttga caggccgggc ggaaacagat cttttttatg atccagaccg acagcccgca
    91981 acgcctctgc aactttgcgc cggatttccg tcgtggatgc accctggact aacaacggca
    92041 gcgaaacatt ctcaaacacg gtttttttcg gtagaagccg gaaatcctgc aagacaatgc
    92101 ccaccttacg acgcaggacg ggaatttcgg acggacgcag tttcgagaga cttcgaccct
    92161 gcacgagaat ctggccctct tccgggcgct ccgccccgat caacagcttg agcagagtcg
    92221 actttcccgc accgctgggc cccatcaaga ggacaaattc gcctttctcg atttccagtg
    92281 tgacatcgga aagcgcgggg cggcggtcgt agtattttga aacgtgaaaa agttgaatca
    92341 tgaatggtgt caacggtagc ggtcagaatg cagcacgagg tcggcccttt ccgcctggga
    92401 tggcacaggc ccggcgcaac acttcagaca ctcaacggca tcctaccatc gcagatcatc
    92461 ccctgcgacg tcgaatgcat tcgcaatatg ctgcacggct gcgccggatc cggcgtcgtc
    92521 ctggatcaat acgacgtcga agcgacaagc acggtcgtgc aatcgatgct gtgcgagata
    92581 gtgagatgcc agttgaatca atttggcacg tttgcgggca tctaccgctt cgatcgcgcc
    92641 gccataggcc cctgatcgcc ggcgtttgac ttcgacaaat accagagtgc cggcgtcgtc
    92701 cgcgatcaaa tccaattcgc ctagagggga gcgcacattg cggcccagga tgcgataccc
    92761 ctgtcggcga agataggctt cggcttgacc ttccccttca tctccgagga gacggcgtcg
    92821 atcacgcatc agtcgaacag gctctcactc tcatctgaca gcgaaaggga gaccgtgtca
    92881 tcctctgcaa agcaatgttg tacaggcgcg aaacttcgcc ggtgaattgc acagggccca
    92941 tgctcgcgga gcaaacgaag atgctccggc gtggcatagc ctttatgctc tgcaaaattg
    93001 tattgaggat accaccgatg atattcgacc atcaggcggt cgcgcgtgac cttcgcaaca
    93061 attgaggcgg cggcgatgga acaagacagc ccatcacctt tgatgatggg acgctgggga
    93121 attgagaggc cggagagcgt gaccgcatcc aggagcagaa agtccggttg caggggaagc
    93181 gccatcaacg cccgacgcat cgccaatagg gaggcttgga gaatattgag gcgatcgatc
    93241 tccacctcag tcgcagcgcc gacgccgaca ccgattgccc gtcgaacaat ttcatcgaat
    93301 agccggacac ggtccgcttc gatgatctgt ttggaatcgt tgagtcccgg taggcggcac
    93361 cgtctcggca ataatacggc cgcggcgacc acgggaccgg cgagaggccc acgtccggct
    93421 tcgtccaaac cagcaatatg ccggtacccg cgcgaccgag cctccacctc aaattcctcg
    93481 gtgggtcccc ctgtcgtcgg gttcaggcta ccaaccaaac gtgtgcctct gtcctgagcg
    93541 tatacgaagg gccttgtcag gcctttgcgg cagcggcagc ttcgaccttc gctgcagcct
    93601 gcgccttgct ttcgactttg aactcgcggt cttccacctt ggcgaactta cccttcttcg
    93661 tccgcaaata atacagcttg gcgcggcgga ctcgtccctg gcggacgacg tcaattttcg
    93721 ccacactggg tgaatggatc gggaaggtcc gttccactcc aacgttatac gacagtttcc
    93781 ggacggtgaa ggtttcgcta ttcagcgtcc cttttctggc gataaccgtt ccttcataca
    93841 cctggatacg ttctttttca ccttcaacga ccttcacgtg aactcgaacg gtatccccga
    93901 tctcgaactt gggcgccgtt tttttggtta aggatcgttg gatccgctct agccgattca
    93961 taatctctct cctcctctaa cgaacaggta cctgtactag actctcttgc atcacttcac
    94021 tcaataaccg ttgatcttct aatccaagct cgcgatcccg caaaagatcc ggccgcttca
    94081 gataggtgtt gcgcaacgct tctttgcgac gccacaagcg gatcacctca tggtgccccg
    94141 ataccagcac ctcaggaacc gccatcccgc gcacctccgc aggcctggtg taatgtggat
    94201 attccaacaa cccttcagta aatgactctt cggccgctga cgccgcatca ccgagtacac
    94261 cggggactaa gcgggccgcg gcgtcgatca tgaccatcgc cgccaactcc ccaccggtca
    94321 acacatagtc gccgacggag atctgttcag gacacagcgc cacattgaca cgctcatcca
    94381 tgccttcata atgcccgcag acgaatacca gcacacggtc atccttcgcc agagactgcg
    94441 caagcgcttg cgtaaacggc cgtccctgtg gagacggcat gatcacccgc aaggcggtct
    94501 cgggatgaga cgcctgatac cgggcgcgca acgcatcgac ggcctggaga accggttcgg
    94561 ctttcatgac catgcccgcg ccaccgccat acggcacatc gtccgccgtt ttatgccggt
    94621 cgaacgtata gtcccgcaga ttctgaacgg aaacctccag caatcccttt tcctgggcgc
    94681 gcttgagaat actctggcca agcaccggtg ccaccatatc ggggaataat gtcagaacgg
    94741 cgcaccgcat attagagatc tccgaaccct tcgggtagcc gaacggtcat cacccgtttt
    94801 ccgacatcca ccgacagcac gatttgtttt gccgcaggaa tcagcagttc ttttccatcc
    94861 tgccgtacga ggaatgcctg attatcagac atagccagaa cctcttccag ctgccccagc
    94921 acggttccgg cttcatcctg gacaaccatc ccgatcagat cgcattgata gtactgatct
    94981 gacggcagcg ccggcgagtc tccacgcgga acctgcaaca gtccaccgcg aaactcggcg
    95041 acctgctccg gcgtcgtgaa cgcttcgaac cccacgatca atgtcggccc acctgaccga
    95101 acatgcgtca cccgggtcac aagactcttg ccgctcgacg caacaatggt cacctcgcga
    95161 agcgcctcaa atcgccccgg cacatcgctg agggaccgca cgcgcgcttc gcctcgcacc
    95221 ccgaaggatc gttcgatcct tccaatggtc acaagctctg cctgatcgag cggcgtcatg
    95281 agctccgcct actttttgct cttctcgtct tttcccggct tttcagcctt cgcactcttc
    95341 tcagtctcga attccttcca gacgccgtgc cgtttcaaca gcgttcgcac cgtcgtcgtg
    95401 ggctgagcgc cgtgccgcaa ccacgaaagc acccgctcgg actttaatac cggcacggcc
    95461 ggattcttca gtggatcgaa aatgccgagg atttctaaaa accggccgtc gcgcggctta
    95521 cgtgaatccg ctgcaatgac tcgatacatc ggtcgcttgt gtcgtcccgt ccgtgccaat
    95581 cgtaaatgaa ccgccactta gaacctccta caataatgag ccctcagcca tggctgggtc
    95641 tgctcggtta acacgctctt cttccccagt gagtacgcag ctcttaccct gctccggtca
    95701 cattccccga agcatctggg ccaactgacg ccgcccacct gctccgcccg acatgacctt
    95761 ggcaattttt cgcgcctgca aaaactgctt gatcaaacgg ttgacctctt gcacgctcgt
    95821 gccgctgccg cgtgcaattc gttttttcct gctgccattg atcaacgtgt gatcgcgtcg
    95881 ttcgcggctg gtcatggaat cgatcatggc cgcgacccgc ttcatttctt tttccggcaa
    95941 cccgctgttc gcgagatctt tcaatttctg tccgcccggc aacatcccca ggatctgctc
    96001 gaaagatccc agccgattca tctgtcccag ttgagagcgg aagtcttcca acgtaaacgt
    96061 actgctcgtc agtttctttt gcgcggcttc cgcctcttcc cgcgaaaacg tctcctgcgc
    96121 tttttcgatc aacgaaagga catcgcccat cccgaggatt cgggaagcca tccggtccgg
    96181 atggaacggc tccaaggcgt cgagcttttc gcccataccc agaaacttga tcggcttccc
    96241 ggttacggcc cggatagaca gaactgcgcc gcccctggcg tcaccttcca ctttcgtcag
    96301 gatcaccccg gtgagaccga cctgccggtc gaactggcca gccatattga ccgcatcttg
    96361 accggtcatc gcatcggcaa ccagcaacac ctcgtgggga gtgacggcct ctttgaccgc
    96421 cacaagttcg gccatcaatt cgtcatcgac gtgcaaccga ccgccggtat cgaggaccac
    96481 gaggtcgtag ccttgttcct gagcgcgctc cactccgcgc tgacagatac ggacaacatc
    96541 agcgcgcgag gcatcgactt gatcaaatcg gtgaacatcg atacccaggt catgaccgag
    96601 gctggccaac tgatcgcctg cggcagggcg acgcgggtcg gccgccacaa gcaacacacg
    96661 tttgccctga ctcttgaata gacgggccag cttgccggag gttgtagttt tcccggctcc
    96721 ctgcaatccg accatcatga gaatcgtcgg cggccgggag gcgaggctga taccggaccg
    96781 ttctccgccc atcatgccgc ggagttcgtc ccacacgact ttgacgacct gatgaccagg
    96841 cgtcaggctt ttcagcactt cctgaccgat cgctttttcc cgaacgcgat ccagaaaatc
    96901 cttcacgact ttgaaattca cgtccgcttc aagcaacgcg agacggactt ccttcagcgc
    96961 ttcggcaata ttctcctccg tgagcacacc ttgcccacga agcttcttga ggatccgttc
    97021 aaatttttcg cttagcgcgt caagcaccaa tcaccaaaca aaaaggccat cgaagccggg
    97081 ttcgagccag agctccgatc gcctcgaaaa acatcaaaaa aatagcatcg ggcagt
//
LOCUS       DAOQDS010000003        95617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_66_length_95617_cov_23.302568, whole genome shotgun sequence.
ACCESSION   DAOQDS010000003 DAOQDS010000000
VERSION     DAOQDS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 95617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 95617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95617
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66_length_95617_cov_23.302568"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1874)
                     /locus_tag="PK589_01055"
     CDS             complement(204..1874)
                     /locus_tag="PK589_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNJ18207.1"
                     /translation="MKRIHGRTRWGRLAAIFGAAAISALQCLTLPAFAGFELPPGERI
                     TNLPAIPRNMPQKEAYELYDPVIGRNFDIKNLWMRADLRVRPEMRNNGCFGLQMNANG
                     TCNTFGTRGAAGAGGNKGNDFFVQQWMRLGIGYDLSPDVNFYVEIIDSATWGGNGTAT
                     NAGNGGDPLNHNCSATATGACRLGVRAAYMLVRNLADVQGLSMKVGRQYVVFGNHSLF
                     GHFDWANTGYSHDGVMFSYTGIKNWDIYAGWFRNSESDLNQAAAVGSGSGNIAGNASG
                     SDANRDADMIVFYNQLKMIPGMVIEPFYVYYKNNYGSSATAGVGNQAFGLGTAKHANQ
                     TRHMIGNRIEMRKGGFDFSNEVAWQFGQMGQAGACVGDQKCLHINAWATRNWIGYTFY
                     DTAWKTRLAFNLDYASGDSRNNTCTNGTNCKTANTFENFFPTNHIHMGYMDVQAWKNM
                     LSPSVNLQARPSARDHIELWYTNLNLANSKDCWYRASQGCYVFSNANNTKTHIGDEID
                     VAYTRMFADGKVALQAAYGTIFSGGYLTSTLNQQVNQHWAYMSLWMNF"
     gene            complement(2124..2423)
                     /locus_tag="PK589_01060"
     CDS             complement(2124..2423)
                     /locus_tag="PK589_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNJ18208.1"
                     /translation="MFGLGAGEILIILVIAFLLFGPKQLPEIGRQVGKAVKGFKETAD
                     DLKKTVEPELNMIQQEMKMVEQDFESSMKEAEEQINHATSGVEHGAEESGLPKQA"
     gene            complement(2517..2801)
                     /gene="tatA"
                     /locus_tag="PK589_01065"
     CDS             complement(2517..2801)
                     /gene="tatA"
                     /locus_tag="PK589_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNJ18209.1"
                     /translation="MFGSFGWMELLLILIIVLIIFGAGKIPQLGEGLGKAIKGFKKSV
                     HEADAIDVTANETEPAAAQPTAQIQQTGQSAPPPPAQQAGAAPPPRTTQG"
     gene            complement(3069..4514)
                     /locus_tag="PK589_01070"
     CDS             complement(3069..4514)
                     /locus_tag="PK589_01070"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ18210.1"
                     /translation="MTPVEDPDALPQPIGEYKPVDYWQAHINTLFYQLRGDQQRSFYQ
                     TFASADYRLAHALAADYFEQAVKRDKKAAAKSSASGDAASRPSLTVMEWGPGNGNLAA
                     CFLSHLQKLDKAGQIYPRVRYVLVDSHAPALERARTHPDLIPHLSKVESLCADVDNMS
                     TIADGTVDRIICNEMWNELATKLVVKKGGEYEEEHLRPNLNERKAAAIADWSAFVRAF
                     DAKDIEKLKQFPPFLDDLIWEREYHKVDWKDVPYRKTLTEFMKAIDEEVLVPVNLGAF
                     GSIKEAKRVLAPDAVGFSSFDAGTADMQVLNDPEKPCYGQFGGQYSFMVNLALIQAVA
                     KHLGITTTIIETQREFVGSRLGTNVMTLMDLLACHPTVGSKVQPWQLDQLTLKTIRTL
                     NEHYESPYQRKIEFPLRSEMPAEEREAAQGTLLSMKPNGIPDTIAYVTEEELSQAQGD
                     LEQLGYEREAWLMALGAPPSPVEYYHFAFRP"
     gene            4718..5278
                     /locus_tag="PK589_01075"
     CDS             4718..5278
                     /locus_tag="PK589_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18211.1"
                     /translation="MLVCPILLFSGSLLWAEDLPLTRNVPIPDFQNRTESAKPYNFDA
                     PPEGMFRHIVLAEGFDEELGFQRTHEIVPVKPTERFSAGARPIFIVFELHQHYQSFQV
                     FGRCYPEEVAGVDGKVVVAEDAMYIALEDQTGYLKLFPTESGWKPGRYKVEIHAGEQI
                     NDMSLMGTMRFTITAASESEVPARSR"
     gene            5303..6133
                     /locus_tag="PK589_01080"
     CDS             5303..6133
                     /locus_tag="PK589_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18212.1"
                     /translation="MTAALPLGYTGLPPQMPGAWAFWRLPVERNVVALQWDILATILL
                     TATIQSLFGVGVLLFGTPILLVLGYDFITALTILLPISLTINLLQVSKDYRYIQWPFY
                     RQILTLSIPCVVLCLLLVTTLKLNIGSIVGLFLIVAALKSVYGPFNRCIESLVRYERI
                     YFTVMGIVHGLTNLGGSLLTAMIHSKPYDKDQTRATTAVSYGTFALFQLITLSMSLGP
                     SAIATSTTALYMAVGVSMFLATELLLYSKMSTERYRTTFAAFLFLSGTALIFKSLFIR
                     "
     regulatory      6249..6323
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            6345..8036
                     /locus_tag="PK589_01085"
     CDS             6345..8036
                     /locus_tag="PK589_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ18213.1"
                     /translation="MNRSPATTPPYPQYQLTEVLRDIFAHLGMLLRLERSILGIIASY
                     AVAIGFFLLCVPIAVQELVSTFSFAMEPRMIFTLALFVASSLTGVAAFRVLQARAVET
                     LQQRIYTRVAIAFTRLLPQLRDDTFATPQAHRFMEADLLTRALVAMVADLFNVAVVGT
                     IGMTMLILFHPYFVLYILVLILGFVGLLTIFGRGGFFITLEMSRLHYEIFGWIQNISH
                     NLPHLRAAGDSPYLLERTDTLTRIYARIRQRRSDTLTGRQYKAAALWQVVGHSGLLIT
                     AGLLVVDGQLTVGQFAAAEVLVGNLLLNMDTLARRMVAMFFTFVSCREMAAVFSLPTE
                     EDGAKEDVPATQFGESGIHLTCRNLSYRAQDGLPIFQNVSLEVAPGENVAILCGTNRA
                     KTALAKVLAGLHPPSTGFVRYNDMNLAEVRRDSISRVRGLILDSHPTLLDGTLEDNIT
                     LGRPAIEYQDLQWALRFVELDHDIDAMPQGLHTPVSSGQTHLSMSQILRLLVARAIVT
                     RPQILIFDGTLHNMLPATRDLLLRRLCAKDEPWSVIFVSNDPTFSTHVDRQVSIE"
     gene            8169..8945
                     /locus_tag="PK589_01090"
     CDS             8169..8945
                     /locus_tag="PK589_01090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ18214.1"
                     /translation="MTVATRPWASVVIPIKDERDNLVPLTEQLVKVLDGREESRSAPF
                     ELIFIDDGSSDGSSDVLDSLAARYQTVKVFHFDRNYGQSAAFDAGFKRSTGELVMTID
                     GDLQNDPADIATLLPHINTFDLVCGWRKDRHDNLTRKISSRIANSVRSAVTGDRVHDT
                     GCSLKLFRRAVVEKMQLFEGMHRFFPALALMHGFTVTEVPVRHYPRTRGTSKYGVGNR
                     LFKGLYDLVAVRWMQHRCLRYHYRGATTSPSGSTSASVPL"
     gene            8942..9241
                     /locus_tag="PK589_01095"
     CDS             8942..9241
                     /locus_tag="PK589_01095"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HNJ18215.1"
                     /translation="MTLDTIWLIIGFLGQGIFFMRWVVQWIASERHAESRVPTAFWYM
                     SMIGGLITLAYAIYRQDPVFIAGQSIGSIVYLRNLMLIHRPNSADSSTASPAIKS"
     gene            9253..11034
                     /locus_tag="PK589_01100"
     CDS             9253..11034
                     /locus_tag="PK589_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNJ18216.1"
                     /translation="MNGEVPSLQTPAPIDRSIQPVTLVVLLALAAVLFFVGLGSLGLT
                     DRDEGRNAEAGREMFETGNYISPTFNYEPRFAKPVFVYWLMTASYHLFGVSEFAARFP
                     SALFGLGLILLQYLFLTRCRGPVIGLFGAAMLLLNLEIIGLSRMALTDSVLIFFTTLS
                     LYGFWLGLYGEGRERHCIWLFYIGMALATLTKGPIGFLIPLLAVGLYLWLTRSWPLFR
                     REGRLLPGLFLFVALALPWYLMMLNLHGQRYTTSAQGDTIGRFFGTMEGHGGTLLFYL
                     PVFILGFFPWSGLLPFAWYQAYRSWREAKQAGTFTPATGTDTPASSQALEWFAAAWVL
                     GGLVFFSLSSTRLPHYIGPLFPAAAILTASFWNRCVTDHAAPHLRAAIHMMTAVGYIL
                     ALAFASLPPLYAKFAVKLVDEFPLAGQVTLGPGPYTVASIFLVGMGLVAYFGLSETRK
                     PAAFWAAGGSLALVVLAVTQLTFPLVNHFVIEPPQRLADVAGLNLGPDDRLILYGQPR
                     PSLVFYAKRKAIVVPKGEEANIKPYLTQPGRTLILLPAAMRNRLPFETMEYPVMLERY
                     GYILLANQSMIHVPEEAEKPPIRIPGH"
     gene            complement(11042..12121)
                     /locus_tag="PK589_01105"
     CDS             complement(11042..12121)
                     /locus_tag="PK589_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNJ18217.1"
                     /translation="MSESGDGQAAPVSTQPAPPPDRPPVLAVILSALVVLGSFAALFH
                     IDIPILRLLRSHNLSSLQSLGDLGEKLGNGGTLITISVLLLGAGYLLKRQAWIRTAVD
                     SLLAHGVVALVVNSLKHIIGRPRPRLTHSGGWHWWPSLQSGLDSFPSGHTSATVAVAT
                     VLARALPRLSWVPFALAAWVGASRIWRGSHFPGDVIGGMVLGFMVGSIFNGPLRWWGS
                     SCAHALVRIAPMVLLLTGWCWVVTHRILDPVTDTVLVASGVMLVLAGWLVQMLPRWRS
                     TSAGGIAAVVTSKGFLLSGVGLATGAPIVIVLTGLLCIARWNAAAQVLEAPAQPSWRR
                     DGLYTAAGLAGVMAIQFLKGLVPLE"
     gene            complement(12118..13245)
                     /locus_tag="PK589_01110"
     CDS             complement(12118..13245)
                     /locus_tag="PK589_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18218.1"
                     /translation="MKHAKQVMAGMLGLMLSLSMTNPAAAASKAAETATPTASTERHL
                     TNIKQLTFGRQNAEAYFSFSGDKLIFQSTNNWMKDTFAAAMHPADIPLGCYQMYVMDL
                     GSEKIRMVSTGSGTTTCGYFFPGDRRVLYSSTHLRGPNCPPKPKREGGAYRWALDDYD
                     LFSVRIDGLDPQRLTNTPGYDAEATVAPNGKTIIWTSMRDGDLDIYAMDLDGTHPRRL
                     TQEIGYDGGAFFSPDSKRIVYRAQHPSNQEELDQYKSLLAQNLVEPGRLEIFIMNADG
                     SNKQQVTSNGASNFSPYFHPDGHRVIFSSNVETRNEGGRPEFHLYLTNEDGSGLERVT
                     VGGKFNSFPMFSPDGKHLVWVSDRNAKEPGEFNVFLADWVP"
     gene            complement(13232..15085)
                     /locus_tag="PK589_01115"
     CDS             complement(13232..15085)
                     /locus_tag="PK589_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNJ18219.1"
                     /translation="MKQPATDRGYGAVALKFSYRGVVDDPPRDPRHLRFVTPSETSGH
                     IGPEGVYLSSESQWYPDVEGSLATYDVTIALPDGWTAVTQGAPQSDQGHWIVPTKSEA
                     LTVVANRFVVKSRTWTAKSGQSIRLATYLFPDEASLADEYLDASARYLDAYIPLLGPY
                     PFSQFAVVENFFSSGLGMPSFTLLGSGVIKRHYTQPYALGHEIVHSWIGNGVFNRIDR
                     GNWVEGLTTYLSNYYYHELMGDEAQAREQRRLMLMGYAVYVRPGEAYPVIQFAQKHDE
                     KDNAIGYQQAAMVFHALRQEIGEAVFWTALKQIPERYLGMAAEWRDLERVFQEAAGRD
                     LRWFFAQWVERAGVPDIEVTGSRVQQSTGTAGDMNRVEAIVHIAQHGTPYRLAIDLEF
                     VLDGGRTHRTRIHLTDAQQNAIIPLPERPLAVRLDPDLHVFRRVARNALAPMLNVYVT
                     DAHRTVVVAPASLQQPGPFTDIVQRIAAQEASKPEPSRTTVKQPEDKTKPTPEGSVLV
                     LGGPGENAAASDMLRYCGDHVHFTEKGFSVEGKSYEGPTMALLVSCRRDDQPGSVVTI
                     LYGVTPPALTRVARLLFFYGWQSYVVFHEGSVVARGDWEDRMTVEVRIETR"
     gene            complement(15455..16240)
                     /locus_tag="PK589_01120"
     CDS             complement(15455..16240)
                     /locus_tag="PK589_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ18220.1"
                     /translation="MTTLLVIDDDRLHCDLLQVALSRHGYRVSTATSGREGVAIFRQL
                     RPVVTLLDLRMPEMDGLAVLKEIRTYDPRAGVIILGGGATEELENLARKLRVTDFFRK
                     GLSLDVLVGAVHRIAQQARREASATQDVTASEIDQPSEEQILVVDDDVMARDLLMRFL
                     SLRGYRVRGAKDGREALRMIEESAPDLLILDLVMPEMNGVELLKVLATREYAGRTIIL
                     SGHQNDPLLEDAWALGPQEVLDKPLDLERALMAIQLVMVCREC"
     gene            complement(16391..17305)
                     /gene="ftsY"
                     /locus_tag="PK589_01125"
     CDS             complement(16391..17305)
                     /gene="ftsY"
                     /locus_tag="PK589_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNJ18221.1"
                     /translation="MGWFQKLSEGLSKTRNAVTGQLDRLLGRAADPALLEELEVALIS
                     ADLGMPVVERVMENLRAQMRGGNFSAAEKVRELLRQSVLDILLPTQGASIDQLIAQGP
                     RPFVILAVGVNGVGKTTTVAKLTQRFRQQGNTALLVAGDTFRAAAIDQLQVWADRTQV
                     DIIRHRPGADPAAVAYDGITAAKARGSDVVLIDTAGRLHTKTNLMDELRKIKRIVAQE
                     CPGAPHEVLLVLDATVGQNAIAQARQFHEAVGVTGIALTKLDGTARGGIVVAIADTLK
                     IPVRLIGVGESVEDLQDFNAKAFVDALF"
     assembly_gap    17317..17416
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17417..18342)
                     /locus_tag="PK589_01130"
     CDS             complement(<17417..18342)
                     /locus_tag="PK589_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNJ18222.1"
                     /translation="MRKIKLREGTNTAVLFGHHDRHLKLIEEEFGVRCSARGEEVTVE
                     GTADAVKQAERVLNELASLTNEGYELRSDDVTHALTALRHNHDASLKELLFSASPIVT
                     RKRFIVPKTPTQKYYLDAIEKHDIVIGIGPAGTGKTYLAMAMAVSALMKKDVSRIILA
                     RPAVEAGEKLGYLPGDMYAKVNPYLRPLYDALFDMMDMERANRLIERGDIEIAPLAFM
                     RGRTLNDSFVILDEAQNATAEQMKMFLTRLGFHSKAVVTGDITQIDLPSDRVSGLIEV
                     RNILQDIAGIEFVYFDERDVVRHRLVQEIIKA"
     gene            complement(18467..19243)
                     /locus_tag="PK589_01135"
     CDS             complement(18467..19243)
                     /locus_tag="PK589_01135"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNJ18223.1"
                     /translation="MADDPDGTEPTKRFQWIVNSAPVLGATGLELDPVHVKRGDQVSL
                     EVVAFDGELESAPYRTEPVSVVNTPPLVSHVTVEADAEHNSRVLAKVDAVDPDRDEIH
                     YNYRWWRNDKQVKEGEESILDTTGFGRKDVVVVEVIARDQDAAAAPVRSAPSALGNSP
                     PLIVSNPAALTNREQYEYVVQAKDVDGDTVSYVLETGPPGMTIDRATGQVSWKVIPGV
                     AGTHRVKIMAEDGQGGIAWQEFELSIPSTAQSQASLPPQG"
     gene            complement(19486..23907)
                     /locus_tag="PK589_01140"
     CDS             complement(19486..23907)
                     /locus_tag="PK589_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18224.1"
                     /translation="MNRRTLASLMIFAGSVSAGLCGSGDAAAVISNADYTAVPPFVSS
                     ATTPNIIILMDNSGSMSNRACESTSCGTLADGSTSTTTTWTNTTRYSGYFDSLRCYTY
                     NTTDKRFESATAKAYVYTDCSTTEWDGNFLNWATLRRFDAVKRAMSGGDCAVNRATDG
                     TCPTSGTPALKTVRAQATGVNDERADVNYSGGTGANTYVGRIPLADRGSNPGTISIGI
                     SASGTPAVPYFCVDNDTSFNSNCGDSYSVRKYELKVGYSTEPTGVIQQIGSKARFGLV
                     EFKSSSEGARMLVGAGSRQSIDWNGTGVETFNTNMAAMVDAVQESYPSTWTPLSESLY
                     ETARYVAQIQSTFVTGYVYPIAFSGGISNGVNFATTGAGSIGTDEITALVGTETCPAG
                     YITNACGRDPYFFGQNHTPPWASTSQVVNCCRTFVIIFTDGEPTQDVNVPTAIQDYAH
                     AHHGQHCNGSDSAAPPRPINGTCNTNAATPFSDLLAEHKTDYADSGSHYLDDVAYWAH
                     TSDLRPCSGTSDGTIAGLSVTGHCIPGTQNLTVYSFFAFGNINGRGILAQTARLGGFE
                     DANGDGIPQTSEWDKENNLTGASTPDGIPDAYFESSNVDDLQDKLLATIASILRRSAS
                     GSSVSVLATASTGEGALYQSYFYPSTIEPSTLSDVKWTGYTQALFIDTFGNTREDTNQ
                     DGRLDYKVDKIIKTRFDSLSNSVKVDKYVDSDGDGLPNDQNSDGVVTLADCNPCGKLL
                     SDILPIWEAGKQLALKDASTRTILTWVDSDHDGVVDSGEQIAFSTANETTLRPYLRAT
                     SSAEGTKIINFIRGCDATTCTDQATTRDRRLQVPSGSGTLKVWKLGDVIHSTPTVVAG
                     PRDRHDAIYGDQSYTAFLQKWYNRRQVAYVGGNDGMLHAFNAGYYHPGDDTSTSAPAS
                     TTEHGWFTTAPADNSSGAPLGDELWGFVPYELLPQLEFLSRADYQHAYYVDLPLKVAD
                     VRIFTADTDHPNGWGTILIGGFRMGGSCGACVAGTGAPPMTVNIGGTNYNFYSAYFAM
                     DVTNPDSPRLLWSFSSSDLGLSTSVPAVVRVNPAADAKASNTNAKWYMVVGSGPTGYD
                     ASVAQGAKVFAIDLATGPGSSNSLVTSYSIGSWDSYIGDMTAFDRDLDYRHDVVYFGR
                     VINDGALPWRGKIYRLTMGAGGATSKFGTVTSPSQWGISSGSARVPTEMLDTFASGAE
                     MGPVASAPSVTTDDAANVWVFVGSGRYYSKSDKTDISTQYFVGMKDSVLNAGCNQSSG
                     ITACMDSDLVDVSNATVCVVGVGDCGLATGTNQVTGVTGATTFTGLIGLVQSKDGWVT
                     KLVEPANPPTKPVPYGIGERAVSSPTVFGGVVFFPTFIPTNDICVSSGTSRLWALFYK
                     TGSAYQEPILGTTGAGANQTVNKFGSLGEGLAFGVVVHMGSGRDGGSPFGLLINMSQG
                     NFGDCATCTTGGGMFGSNISVPVAIDPRSRFFSWTNQ"
     gene            complement(23985..24320)
                     /locus_tag="PK589_01145"
     CDS             complement(23985..24320)
                     /locus_tag="PK589_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18225.1"
                     /translation="MTMNHLILLLILSLSMLVQAANPGPSVAGMEPEQAQEPILFSTV
                     GFQSGVIDEVQGSTIRIDGRTYVLKSAVVVVTHEGEPLAVERIIPTSLVKFHLKEGHI
                     DKMMVTLPQ"
     gene            complement(24329..24910)
                     /locus_tag="PK589_01150"
     CDS             complement(24329..24910)
                     /locus_tag="PK589_01150"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HNJ18226.1"
                     /translation="MTGQQGIALLTVMLMLLILSILGIASITVTSMENRMAGFFRTTE
                     AVVAAADSCEGLAANLIQQTLSPPGVLPTSFVAPTGPVPAANATILWQEIIGSSPLPS
                     PAPTGTLAENYADSAAMDPNFVMTNVAGFTVNGDIDRLYTHSKAGQGTGVTSTEFVYR
                     ITCTATNATTGSSSTVTSVYGCLDGDTCVKKVN"
     gene            complement(24979..>25341)
                     /locus_tag="PK589_01155"
     CDS             complement(24979..>25341)
                     /locus_tag="PK589_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18227.1"
                     /translation="CDGCNQAAPNPLYPDGIIDDQDGSSSSGFPTFTQGDFVSNGSWA
                     ITPRTPDKIRMAQVSLVVRPTKADDGLDEKGTKAVNTTGPVIVGDHNPSTDTGYNAQT
                     YQQQRRRVVIRTVQPRNL"
     assembly_gap    25342..25441
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(25599..26123)
                     /locus_tag="PK589_01160"
     CDS             complement(25599..26123)
                     /locus_tag="PK589_01160"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNJ18228.1"
                     /translation="MSYTTEYIASSQLITPRQLGFTLLEAMIAAGVLSVGLLGLAGLS
                     GMSLGKNVDANDMSRVTNIAMDMAERIQSNRQRVLDYNATATNVACPQSVSTQRMAWG
                     DCTQWSALVTNSGLQSAVGTVTASRLDPDPTANPVTMNRFLVTITVNWRTQKTDVSTS
                     RNKSAVFTTVIAPE"
     gene            complement(26131..>26508)
                     /locus_tag="PK589_01165"
     CDS             complement(26131..>26508)
                     /locus_tag="PK589_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18229.1"
                     /translation="LAMVAVPNYLQWNARYQLKQGTTELAGSLTLARMAAMNRNLAVT
                     VTLALASGRVTVDFGGALAPIVLPQAIVGFTGGPTVQFTRQGLSGSTANVPLTLVSQQ
                     GTVYSLVVTPSGKVNWCAHATCP"
     assembly_gap    26509..26608
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(26664..28463)
                     /locus_tag="PK589_01170"
     CDS             complement(26664..28463)
                     /locus_tag="PK589_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNJ18230.1"
                     /translation="MIRASSPACLSNGLTLGKPRPTRSRPSGLYEPLVHQGFLRQEEL
                     ALAFSEALRRRLDVTTLLMDRYRIPKPAIGAVLAEFYECQFLAYDEQTIVEPELLKNL
                     SIDYLRMNHWVPLKRQAQGVEVLIDDPHNPDKLLDVRRAFPGQSVLYRVGLRADIERL
                     LSDACGREVGDPITDILGELVSEAQLEEQQNATIAAITENDSAIVRLANQIIAEAYRR
                     GASDIHIEPYADRKETAVRFRVDGTCFTYMKIPAAYRRAIVSRVKIMASLDIAERRKP
                     QDGKIRFKLTTGQEIELRVATLPTAGFNEDVVLRLLSANGPRRLEEFEFSVETRRVVE
                     QLAQKPHGIVLCAGPTGSGKTTTLHAILASINTDERKIWTAEDPIEITQDGLRQVQVH
                     PKIGLTFATTMRAFLRADPDVIMIGEMRDKETADIAIEASLTGHLVFSTIHTNSAVET
                     VVRLLDMGCDPFNFSDAMLGVLAMRLCKRICSHCREPYHPTRSEYDELVHAFGERDWA
                     SRQAAHDDTFTLFRGRGCEVCHHSGYRGRVPIHELMVVSDGMKALIQGRGRTGELLAL
                     AKSEGMQTLVQDGIGKVLQGLTTYQQVRAVAIK"
     gene            complement(28531..29964)
                     /locus_tag="PK589_01175"
     CDS             complement(28531..29964)
                     /locus_tag="PK589_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18231.1"
                     /translation="MALLAVLMVVFLLTLLGMTSMQLAGQEVVGASALQEERLAHHAA
                     EAAVDVVMGWFHDPTLRPQEIGTTWFSKRLVNAQGDPSYVDAQGHGQFIGTSTAPDVV
                     FDAANIQHDRLLNDPQTGWFKSLKGLAKILKLRVYGATRPGLLCTVEVTAGAGPASRV
                     TKTLSLEFGAYAVPALHAPLQSGTLGSEAVQSRSGSVFTHWGEMVVRGRAYLPRPEDV
                     PVKSALAPVSGQVYDEMSHREDRWLTIRLGGDAFFAQLPAEAWSGVPLNVHAHQEPVP
                     GIKVDQWEYATLKRMAMQFGQVYGIDREGLLYAGGVIQAGMGRPASEVFASKGPGDHR
                     GLVFVDTLDGMPPRFDNLGTIVLDQDYAEGIFIVNAHVLWKAGRSGRALPALSPPPEG
                     QQSLGARVPVQLSGIHLQGVLYAAGDVRFAGHLKVYGGVAAQGAIMEAANGSGMLEVW
                     YNHDLREGFVQGLPVVFAAPGSWQTKL"
     gene            complement(30046..30819)
                     /locus_tag="PK589_01180"
     CDS             complement(30046..30819)
                     /locus_tag="PK589_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNJ18232.1"
                     /translation="MTPATLSDSQQTKPIRARWCLNTRGVSLIELLIALAISSVAIAA
                     AIQTSAAYGSRLSSQHATMTANQELRLGLDVLCSELRLAGGGLLSGDTVFSKMEVDEV
                     EFFANLNGSSTILTQIVEVGRQDLPVEDGADWPKGKQLLLCTAAHCVWNRLAADGRKQ
                     TLTLMTPTLEQYQPKTAIFLLNRVRYYLKRAEGTHRLMREVDGVASTLLSELSGMQLQ
                     YFNKSGGIANDARDVVRVRVTIQGARQGMNLSRDIAIRM"
     gene            complement(30816..31343)
                     /locus_tag="PK589_01185"
     CDS             complement(30816..31343)
                     /locus_tag="PK589_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNJ18233.1"
                     /translation="MKKPEGKQATWQGLTTSTAGFTLVESMVALVVLSIGVIGTIAMC
                     EWAQRGLQRGAMTTTALALAESCLEAKRSLPWDQLLVDDLDQNGTLESLMRDDGLLDD
                     ETNGDGIFTGSVTRSNIRLVWTVELNRGGQPGTASLATIEARASFTTLGGQSREVRVR
                     TIRANPRAVGAMALS"
     gene            complement(31340..31774)
                     /locus_tag="PK589_01190"
     CDS             complement(31340..31774)
                     /locus_tag="PK589_01190"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNJ18234.1"
                     /translation="MNERGVSLVELCTVLAIVAIVIGVSVPGWSALVAKQRQRAVVAE
                     IASELRMARQLAMARHERVRVVVNAAQSELRTECVDCETTVLRRYEFARTGTVVDSMS
                     TRPEILFQSSGRSATATTVVLIDSRHVTHQVTVSLTGRVSLS"
     gene            complement(32069..32857)
                     /locus_tag="PK589_01195"
     CDS             complement(32069..32857)
                     /locus_tag="PK589_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNJ18235.1"
                     /translation="MAGGVMIPYVMVFLFGAVIGSFLNVCIYRLPREESVAWPASHCP
                     ACRQPVAVYDNIPVVSYLLLRGRCRSCQVPISTQYPLVEAVNAIGYVLIFWMFDFTAE
                     AFVYSALLSALIVITGTDLSHTMIPDAVTLPGIAIGLICAAVILPIGLIDSLLGVLAG
                     GGILWFLAWVSPYVFGKEGMGGGDIKLMAMVGAFIGWQPVLLAIMIGSFLGSLVGVAL
                     IAAGLTKRDQYIPFGPFLAAGSLLALLFHQPLLNWYWSLISLPQ"
     gene            33062..>33324
                     /locus_tag="PK589_01200"
     CDS             33062..>33324
                     /locus_tag="PK589_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNJ18236.1"
                     /translation="MPTAARTTKGRTKAASSEPILPPRKRRPEALTTREQEILELIWT
                     GFKNKEIAQRLKISVKTVEAHRANMMKKIRVSNTAQLLKAAIQG"
     assembly_gap    33325..33424
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(33512..34294)
                     /gene="rlmB"
                     /locus_tag="PK589_01205"
     CDS             complement(33512..34294)
                     /gene="rlmB"
                     /locus_tag="PK589_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNJ18237.1"
                     /translation="MAPVDGKDNTPDVIYGLHAVREALRAGARPIQRLLVLGTDRQFG
                     EIVRLAREQRIPVYVEPRAAFDRLVPSGHHQGVVGLVAAKSYSDPDAILASAQAPGQT
                     PLLVLLDGVEDPHNLGAIVRTAEASGVQGVFIPERRAVGLTSVVAKASAGAVDYMPVG
                     RVPNLSRWIERLQQAGIWVYALDAEAPKVYTKLDFRGPVALVFGGEGKGVRPGVRGAC
                     DDAAHIPMSGKVSSLNVSASAAIVLYEAVRQRRERGEDRVGH"
     gene            complement(34269..35729)
                     /gene="cysS"
                     /locus_tag="PK589_01210"
     CDS             complement(34269..35729)
                     /gene="cysS"
                     /locus_tag="PK589_01210"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNJ18238.1"
                     /translation="MLRIYNTLTGSKEPFESMVPGKVRMYVCGVTVYDYCHIGHARSA
                     LVFDVLRRYLEYSGFAVEFAKNFTDVDDKIIKRANEQGVSCDQITATYIKAYYDDMER
                     LGVRRATLEPRATEHIADIVALVDTLVAKGMAYRVNGDVYFQVDRYPGYGRLSKRNTD
                     DLQAGARVDVDERKRHPMDFALWKGSKPGEPSWNSPWGPGRPGWHIECSAMAMRHLGE
                     TFDIHGGGMDLIFPHHENEIAQSCGATGKEFARYWVHNGFVQINQEKMSKSLGNFFTI
                     REIFDKSEWPDAVTGEMLRYFLLSTHYRGPLDFSDQSLAEAKNALNGLYDLFERLKEP
                     APSHGAADQQMREASIRTRQAFADAMDDDVNTPNAVAALQKLRGDANKALDAGLSAEM
                     RHAVRNEFRSLGVVLGLFQQDSWQFKSPGSRETQGASGVSAKSLSDEEVADKIAARLA
                     AKQSKNYKLADELRAELASHGIIIEDRPDGTSRWKR"
     gene            complement(35739..36374)
                     /locus_tag="PK589_01215"
     CDS             complement(35739..36374)
                     /locus_tag="PK589_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNJ18239.1"
                     /translation="MSALIEWLIEVLGRFVIATISTFGYTGIVLTMAIESACIPLPSE
                     IIMPFSGYLVFTGQFSMVGVTLAGAIGNVLGSLVAYYVGVWGGRPFVERYGRYFLVSS
                     HDMELADRWFAKHGEAAVLIGRLLPVVRTFISLPAGISRMDVKKFVVYSFVGALPFCY
                     ALAYVGLKMGEHWNELHQYFHHADLVIGVLLAMGLGYFLWSHWPRRRTSME"
     gene            complement(36371..37186)
                     /gene="surE"
                     /locus_tag="PK589_01220"
     CDS             complement(36371..37186)
                     /gene="surE"
                     /locus_tag="PK589_01220"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNJ18240.1"
                     /translation="MARVRILVTNDDGIASPGIHAVAAALGTLGEVWIVAPDRERTAV
                     GHAVTLHKPLRITKMAPRVFMVNGTPVDCVNLALVKILPGPPALILSGINRGVNLGDD
                     VMYSGTVSGALEGTILGIPSVAVSQEGGETFRFEVGARYATRVAAQVLQHGLPAETIL
                     NVNIPDVPFRSIKGVKVTCLSRRRFNNPIVEKVDPRGRKYYWIAGTRQSWSRQQDADH
                     EALERRMVSVTPIHLDTTYHAMLEHFKAWEQPLSKLAASRRVVKARSSRRTRA"
     gene            complement(37349..37425)
                     /locus_tag="PK589_01225"
     tRNA            complement(37349..37425)
                     /locus_tag="PK589_01225"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37389..37391),aa:Pro,seq:ggg)
     gene            complement(37488..37841)
                     /locus_tag="PK589_01230"
     CDS             complement(37488..37841)
                     /locus_tag="PK589_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNJ18241.1"
                     /translation="MGNDPRLGSKVFYKIGEVSKISKLPAYVLRFWESEFTFLKPKKS
                     RGNQRLYVQRDVDTVLEIKRMLYDEGHTLAGVKRYWARRGRATVKKSGSRKELAQRVR
                     GDLQAIIRMIDSHSA"
     gene            complement(37883..38158)
                     /locus_tag="PK589_01235"
     CDS             complement(37883..38158)
                     /locus_tag="PK589_01235"
                     /inference="COORDINATES: protein motif:HMM:NF001401.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNJ18242.1"
                     /translation="MRKADIANEIFKQVGVSKNDAADIVELVLNLLKGVLQKGDAVKI
                     AGFGNFVVRSKGARKGRNPRTGEEIGITPRRVVTFRPSQVFKKYVNS"
     gene            complement(38304..39947)
                     /gene="cimA"
                     /locus_tag="PK589_01240"
     CDS             complement(38304..39947)
                     /gene="cimA"
                     /locus_tag="PK589_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNJ18243.1"
                     /translation="MKRRTTASRRSSAPDSTTAPTLTAAQASALEIYDTTLRDGAQAE
                     DVSFSVEDKLRVAQQLDELGVHFIEGGWPGANPKDIEFFRQIKTMPFRNATVVAFGST
                     RKASNPVRKDKNLQALLDSGTQTITLFGKSWSFQVTDALGIALAKNLELIEDSIHYLR
                     SKDRRVFYDAEHFFDGYKANPDYALQTIRRAIAGGAERVILCDTNGGTMPWEIREICR
                     IVKQECPVPLGIHAHNDSEMAVANSLVAVESGILQVQGTINGIGERCGNANLCSIIPN
                     LQLKMQRPALGNKLTRLKDVSGFVTEIANLMPNKRQPYVGDAAFAHKGGVHIHAVLKN
                     AATYEHIDPAVVGNRRRILVSDYAGRSGLMEKVEAYGISLTKNHAKLQELVETLKERE
                     SQGYQFEGAEGSFELLMRKAMGSHRPSFQLLGFRVIVEKKQEHGLLLSEATVMVKVGD
                     VVEHTAAVGAGPVNALDHALRKALEKFYPQLREVKLLDYKVRVLSADKGTESKVRVLI
                     ESGDHKDKWGTVGVSENIMEASWQALADSIEYKLLLAGR"
     gene            complement(40022..41257)
                     /locus_tag="PK589_01245"
     CDS             complement(40022..41257)
                     /locus_tag="PK589_01245"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNJ18244.1"
                     /translation="MALIVQKYGGTSVGNIERIHRVAERVERAQKDGHQVVVVLSAMS
                     GETDRLLKLAHEATHSPDERELDMLLSTGERVTIALLAMELRGRGVNARSFTGRQVGI
                     HTDSAHTKARISRVTAERIKEALAAGVIPVVAGFQGINASSDVTTLGRGGSDLTAVAL
                     AAALKADRCIIYTDVDGVYTADPNIVPAARRLDKISYEEMLEMASLGAKVLQSRSVEF
                     AAKYSVPVEVNSSFKEGKGTLVTREDADMEGVMVSGVTGDRNQAKLTIVGVPDRPGIA
                     ARVFGAVANANIVVDMIIQNVSQASMTDISFTVPKADLRNAVDLVQRLSQEIGARSVA
                     VTESIAKVSLIGVGMRSHSGVAAKMFEVLSREGVNIMMISTSEIKISCVIEEKYLELA
                     MRTLHTAFGLDRVSAPALG"
     gene            complement(41263..42513)
                     /gene="apgM"
                     /locus_tag="PK589_01250"
     CDS             complement(41263..42513)
                     /gene="apgM"
                     /locus_tag="PK589_01250"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNJ18245.1"
                     /translation="MKYLILHADGMADLACAELGGSTPLQAAKTPFLDQIAQRGEVGL
                     LSLPSDAGRTGSDVTGVAVLGYDPKKYYPGPGPLEAAGLGVTVGEQDVVFRCTMVTVR
                     GESASGGTDRAADIKKLGPHVVMEDATAGLIETEQARELVEAVNEQLGSESIQFYPGS
                     GHRHLMVWVGGKSRATCVDPQQLVGQSIAEALPAGDGADVLRKIMDASFFILRDHPVN
                     EEREEDGLKPANCLWLWGQGRAPLWPPLPERYQVSGVVVSASDVHRGVGLCAGLDAAE
                     LPAESGDESETFAGCVKAVVQELAKKDFVYLHAGLSDDVLHATNVHDKVQAIERFDAQ
                     VIGPILSALAKYPAYRLLVVCDPGLAKGQGSDVPPILYALCDGATQPPAGSSTRFHEQ
                     DKTIAGSAPRDATKLMMRLFPRGA"
     gene            complement(42532..43590)
                     /gene="thrC"
                     /locus_tag="PK589_01255"
     CDS             complement(42532..43590)
                     /gene="thrC"
                     /locus_tag="PK589_01255"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNJ18246.1"
                     /translation="MTRWRGIIEEYRKFLPVTAQTPVITLGEGNTPLIRATRLAKKIA
                     PGVDLYLKYEGMNPTGSFKDRGMTMAISKAVEGGARAVICASTGNTSASAAAYGARAG
                     IAVYVLIPAGKIALGKLSQAMMHQATVIQVEGNFDQALTIVKELSATYPVELVNSLNP
                     YRIEGQKTAAMEICDDLGDAPTVHVLPVGNAGNITAYWNGYREYRAANQTTRLPRMMG
                     FQAAGAAPIVLGHVVKEPQTVATAIRIGNPASWQSALTAVKESSGAIDMVTDEEILHA
                     YALVAREEGVFCEPASATSVAGVIKLSQAGQLKEGATVVCTLTGHGLKDADTAIGISR
                     QPQTVKATRDDVARLLHV"
     gene            complement(43638..44951)
                     /locus_tag="PK589_01260"
     CDS             complement(43638..44951)
                     /locus_tag="PK589_01260"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNJ18247.1"
                     /translation="MKTEIGVGLIGFGTVGTGVARVLIENAELIRRRVGVPIKLVRIA
                     DLDITRDRGVAIPSGVLTTDIQQVLEDPRVDIVIELMGGYDLAKRVILDAVQRGKHVV
                     TANKALLAVHGEEIFAAASRRGIDLGFEASVGGGIPVIRALMEGLAANNIQSIYGIIN
                     GTSNYILSRMTSEGQRFEVVLDEAKRAGYAEADPTFDVAGIDSAHKLTIMVSLAYGTP
                     VNFKEIYTEGITGLTPLDIAYAKEFGFTIKLLAIAKCSDGEIEARVHPTMVPSASQIA
                     KVDGVYNAIQLVGDAVEDVVLYGRGAGSMPTGSAVVSDVMSIARDLLKDATGRVPPAS
                     YQPDYRRPLRIRPMEEITSLYYIRFMVLDRPGVLSQIAGVLGRYSISISSVLQQGRKE
                     GQTVPVVIMTHMAKERDIQHALREINPMPYISEPTTLIRVEGKDE"
     gene            complement(44948..46144)
                     /gene="alaC"
                     /locus_tag="PK589_01265"
     CDS             complement(44948..46144)
                     /gene="alaC"
                     /locus_tag="PK589_01265"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HNJ18248.1"
                     /translation="MSISDGFYRIKRLPPYVFAQVQSLKLEARQRGEDIIDFGMGNPD
                     QPTPPHIVDKLIEASRKAKNHRYSASRGITKLRHAITGWYKRNYDVDLDPETEAIVTI
                     GSKEGLAHLALATIGPGDVVLTPTPTYPIHMYSFIIAGGEVRGIELRQDSDFFDDLLR
                     VYRQTLPRPRILVINFPHNPTTAVVDLEFFKKIVAFAKEHDVIVIHDLAYADIAFDGY
                     KAPSFLQVPEAKDVGVEFYTLSKAYNMPGWRVGFCVGNREVVGALAKLKSYLDYGIFQ
                     PIQIASTVALNGPQDCVKDVVQRYQNRRNVLVNGLNRIGWPVALPRATMFVWARIPDP
                     FRHMGSLEFSKLLLREAKVAVSPGIGFGEGGDEFVRFALVENEHRTRQALRGIRKVLN
                     LDDQEP"
     gene            46446..47159
                     /locus_tag="PK589_01270"
     CDS             46446..47159
                     /locus_tag="PK589_01270"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNJ18249.1"
                     /translation="MARLGVNIDHVATLRQARGGTDPDPLTAAILVELAGADGLVVHL
                     REDRRHIQDRDLTILREIVRTKLDLEMAADDAMAKIALSVKPDLVTLVPERRQELTTE
                     GGLDVANHRERIQKIVDLLRDGGIPTSLFIEPSLDQIKAAHKIGAAYVELHTGRYANA
                     NRSKEEDEEFEAITQAAKLAYKLGLGVNAGHGLTYKNVKRLAKLPEIVEFNIGHSIIA
                     RSVMVGLVQAVREMKELVA"
     gene            47231..48775
                     /locus_tag="PK589_01275"
     CDS             47231..48775
                     /locus_tag="PK589_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNJ18250.1"
                     /translation="MTAEQMRALDHRTITEAQVPSLVLMERAGTGVVTHLEARYGSVA
                     GKKIVILCGKGNNGGDGFVVARLLRRKKARVHVLLLAPVAELSRDAKAMYQRFHRTAG
                     PSSITRPSDTNAIQQRLTPSEIIIDAILGTGLSTPVTGLYRDAIDAINAAGRLTVAVD
                     LPSGLHADTGSVLGTAVKADLTVTFGLPKAGLYCGGGIDCAGLVRLADIGIPSSFVDA
                     VGSRLLLLTAASANAALPPRHPSSHKGTYGHLGIIAGSVGKTGAAAMAAMAALRIGTG
                     LVTAAVPSSVNDILEAKLLEAMTLPMPETKARTFARSGLDRLLAFAGPRDAVAIGPGL
                     TTHPETVDLVQEFVKRIDKPCVLDADALNALAGKASLLTECPRPPIITPHPGEMARLE
                     AEATTQSVNEDRLGTATRFARERGVFVVLKGARTVIARPDGVAAICPTGNPGMATAGT
                     GDVLTGMVGGLLAQGVAPWDAACAATYFHGLAGDLAAQQLGQAGMIARDLIRHIPHAI
                     APTTHL"
     gene            complement(49068..49208)
                     /locus_tag="PK589_01280"
     CDS             complement(49068..49208)
                     /locus_tag="PK589_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18251.1"
                     /translation="MGKINLKPIMRKPCAPAFGPFNSENSAAVYVVTQARILELGGTE
                     AV"
     gene            49365..49709
                     /locus_tag="PK589_01285"
     CDS             49365..49709
                     /locus_tag="PK589_01285"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNJ18252.1"
                     /translation="MIRLILVGTVLLLALSFFLQNQEQEVTLRYFFGLRSASILIYKP
                     ILAAFGVGLLVSGILLFPAWVRGRIELRRKTKALQEAEVDLERLRQALDKAARRVGSS
                     SDIPTEGEPVDG"
     gene            49702..52350
                     /gene="mutS"
                     /locus_tag="PK589_01290"
     CDS             49702..52350
                     /gene="mutS"
                     /locus_tag="PK589_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNJ18253.1"
                     /translation="MGEADGTPLMRQYREIKQAYRDAILFFRVGDFYEMFQEDAVEAS
                     KLLSIALTSRDKSSETPIPLCGVPYHAATNYIAKLLRAGRTVALCEQVEDPKLAKGLV
                     RREVVRLYTPGTLIDSEFLPSSESNLLAAVAIRLRPATHEPTGCSFGLATLEVSTGEY
                     WLCEFHGPQAQIALMDELARLDPKELLYAEGCTPGAQSWIAELIGPRCCAQPATWFEL
                     DTGRAMLQEHFRVHSLDSFGCQQLTLGIQAGAAVLRYVRETQPSASLDHIRNLRVRQP
                     HDAMHLDSVTIRNLELVKPAGRWDDAPNHRSPYTLLGVLDRTVTAMGSRLLREWLLRP
                     LINCAPIEARLTAVEELKESIEPRVRLRTALRTVQDISRLCSRISLGVANPRDLLALK
                     LSLSSLPAIQAELTPLQAPLLAELSASWDNAQDLYELIEQAILADAPVSIRDGGILKD
                     GYHPDVDELRKATREGKGWIAGLEAKERERTGAESLKIRYNQVFGYYIELTKANLGKV
                     PSDYIRKQTLVNAERFMTAELKELEERVTGADAKLVALEQSLFEQLRTGLAAETARLQ
                     EISRRLAVLDVLTALAETAGLNRYVRPTIDTGDVLHIKQGRHPVVERLDLSGGFIPND
                     THLDLHTSRLHILTGPNMAGKSTYLRQVALIALMAQIGSFVPAAEARIGLVDRIFTRV
                     GASDNLAGGQSTFMVEMTESAHILHCATARSLILLDEIGRGTSTYDGLSIAWAIAEFI
                     QDPQRLGARTLFATHYHEMTQLEGLRAGITNYSVAVKERDGKVLFLRKIVQGGADRSY
                     GIHVAQLAGLPDAVIQRAKAVLAQLESTTAPRPILESQQALQFDSTLPAPHPMLEEVR
                     QMDLFSMTPLDALNRLAALQQRLLSE"
     gene            complement(52457..53131)
                     /locus_tag="PK589_01295"
     CDS             complement(52457..53131)
                     /locus_tag="PK589_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18254.1"
                     /translation="MMKLQKQGWTFMAAAALVVSFASTALAIEAFQERFEWGDLSKPT
                     TLQGRVIILDPYDEAVWLNIAVFGGNAESGLWWQRVHPGKNLKFYPADKNVWEQLKSM
                     ARAHSGPAAAKEVPPGSPTTLVEFVAQETEQNHRVITSVKKLNDNAGEMGKPKSISSL
                     RLKECEGKTEVDAACHAAKTLLNTSPKTPDGANLPLQYDVLLPDGKPIANLIPWTAKY
                     NQGSKH"
     gene            complement(53189..53701)
                     /locus_tag="PK589_01300"
     CDS             complement(53189..53701)
                     /locus_tag="PK589_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18255.1"
                     /translation="MNRVGILAALAVSSVVGLLTAGVSMAADAPAGGGPSEVTTGSGK
                     FFKGEPTNTLKGRVWSQWADNPDGEILFGIQYWNTGDPASGEGGGRSSTSMDVKPPDP
                     LFTCCAWGYTGGTDKNNPYAGWHHAQTTVRLAVKDKGLMDQIIKASQELVAMEVTLDG
                     RTITGFKVLK"
     gene            complement(54071..55324)
                     /locus_tag="PK589_01305"
     CDS             complement(54071..55324)
                     /locus_tag="PK589_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ18256.1"
                     /translation="MYRKIVLDNKLRVVAELLPTLKSVTIGIWVNVGSRDEQPGEEGL
                     SHFLEHMFFKGTRSRSATQISREIDALGGEMNAFTTRETTTFYVKVLDQQLEAALELL
                     SDLFYRSRFESKEVEKEKQVVLEEIRMVQDDPEDLVQELHMKHTLGSHPLGRPILGQA
                     PRIQALGRQDLVSYVGSHYDPERTVVAVAGNFTWKRLEQLLARYFSAPHKGVAVKQSR
                     RPPEVRGGVLVRRKALEQVHLCLGLQGLGAGHKDRYAAHALNGVLGGSVSSRLFQEVR
                     EKRGLVYSIYSFLSTYSDGGMTTVYAGTRPKEVERVVEVVCRELKKLRSKGIDAKDLA
                     RVKNQMKGSLMLSLESSHSRMSKLAKDELTQGSHVSLEQMIGEIDRVTTDQVYRVAQT
                     LLDQRCLAITALGPIPVNSLQSFAS"
     gene            complement(55331..57448)
                     /gene="pnp"
                     /locus_tag="PK589_01310"
     CDS             complement(55331..57448)
                     /gene="pnp"
                     /locus_tag="PK589_01310"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNJ18257.1"
                     /translation="MKQVVDIELAGRRLTLETGRIAKQADGAIWATYGDTVVLATAVA
                     SQNAKPGVDFLPLTVDYQEKTYAAGKIPGGYFKREGRPSEREVLTSRLIDRPLRPLFP
                     EGYYFETQVITSVLSADKTGVSDVIAITAASAALAISSIPFNGPIAGVKIGRVNGQFV
                     VNPDLETLETSDLHLVVAGTADAVMMVEAGANELPEATMLEAIELAHSEIKKIVAKIE
                     ELRTLAGKPKRVVTQEPIDAALAEQVRKLVAGPIREAILIPNKSARQERLDQVLAETL
                     VALKSEEPNRDRHVKIIFHGLEYTEVRNMILEKRVRADGRGPSDIRPITCEVSVLPRA
                     HGSAVFTRGETQSLAVVTLGTTDDEQRIDALEGEYMRTFMLHYNFPPFSVGEARPLRS
                     PGRREVGHGALAERALKSIIPGKDKFPYTVRIVSEILESNGSSSMATVCGGTLALLDA
                     GVPIKEPVAGIAMGLIKEGDQVLVLSDILGLEDHLGDMDFKVTGTKNGVTALQMDIKI
                     GGIDAALMREALAQAKAGRLHILGCMAQALTAPRAKLSAFAPRIFPMKIKQDKIRDVI
                     GPGGKMIRSIIAETGVKINVEDTGDVTIASSDEASAQKAIDMIKRLTEEVEVGKIYLG
                     TVRKIMDFGAFVEVLPGTDGLVHISQLAHHRVKAVTDEVKEGDQIQVKVLEIDKQGKI
                     RLSRKEAMPAPAGSPATEPTPAG"
     gene            complement(57608..57877)
                     /gene="rpsO"
                     /locus_tag="PK589_01315"
     CDS             complement(57608..57877)
                     /gene="rpsO"
                     /locus_tag="PK589_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNJ18258.1"
                     /translation="MALVKEVKTELMKNFQQHDKDTGSPEVQIAILTNRITYLTEHFK
                     LHKKDHHSRRGLLTLVGRRRRLLDYLRHVEEGRYRAVIERLGIRK"
     gene            complement(57884..58912)
                     /gene="truB"
                     /locus_tag="PK589_01320"
     CDS             complement(57884..58912)
                     /gene="truB"
                     /locus_tag="PK589_01320"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNJ18259.1"
                     /translation="MMIASTESQTATMLQDGVLNVRKEAGWTSHDVVARVRGKLRGMK
                     LGHAGTLDPAATGVLPLLVGRGTRIAEYLLEWDKTYLAGLRLGETTDTQDATGTVLQR
                     SPITALTEARIRETIAGFEGRIQQVPPMYSAVKVGGVPLYKAARAGKDVARQPREVTV
                     FRLEVVSIQLPDVMLRVVCSKGTYIRTLCADIGEKLGVGGHMATLIRERVGPLMVEQS
                     LTVNEVETRLTQGTLAGSMLTLDEALVGLPVCIIGRETARQVVHGMAVPTADVLSWQG
                     LPTILPEASGGPIRIKDTAGRLLAIGTMPAGLGDQARMPGELSIAMSKVLVEDESQGS
                     SIANSIEE"
     gene            complement(58916..59314)
                     /gene="rbfA"
                     /locus_tag="PK589_01325"
     CDS             complement(58916..59314)
                     /gene="rbfA"
                     /locus_tag="PK589_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNJ18260.1"
                     /translation="MTKSTYSRAERVADQIRMEVADILMRKIKDPRVRSVTVTDVELT
                     KDLRIARVFVTTMEQNKDERLVFDGLAKASGFVRAELGRRLALRYLPELIFMKDVSGP
                     RADRVLALLDGLHLDETKEDRLPETPRTDV"
     gene            complement(59324..59605)
                     /locus_tag="PK589_01330"
     CDS             complement(59324..59605)
                     /locus_tag="PK589_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNJ18261.1"
                     /translation="MIVGLCTVELFIPDGHSLKDKRQVLQSLKSRLRDKFNVSVAEVG
                     EQELWQKAILGLACVANESAHVNQVLDQAVNLIQGVPTIQLLRSRIELL"
     gene            complement(59602..62187)
                     /gene="infB"
                     /locus_tag="PK589_01335"
     CDS             complement(59602..62187)
                     /gene="infB"
                     /locus_tag="PK589_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNJ18262.1"
                     /translation="MRVYELAKQLGMENRELIPELKRLGIPVASHSSALDDDSVRMAL
                     EKLGSKARAGETSSSGHEAKKMTRLPKESAGSHAAAHEEAPKPDKKRILIKKKREEGA
                     EDVAASLAAAEAVFAPAAPAAADAGVVDQAPLPGSEVSEAGPIEPVQAQEPEPPPPVV
                     ATSEDVTTKPSQAPVVTTPPVDPLAAIKKKSLVPDASESEAAAREKLKKAKKAPRTRE
                     EDEAKFKNDATRWGDLRAIPVQRREDRSKHIHHASPTEITKPRKKSVKLTAGVSVKEF
                     AELIGQRPADVVRKLMDMGQMVTFNQSINLEAASLIAEEYGARVEVSTEKVGEALLEE
                     AAQSSGEEHAVSRPPVVTIMGHVDHGKTSLLDAIRSTKVAEGEAGGITQHIGAYIVGV
                     HDKQVTFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTVEAIHHAKAAGVPL
                     IVAINKVDKPGANVDRVKNALTEHGLVAESWGGDTIMVEVSAKQRTGLDQLLEMILLQ
                     AEVLELKADPTRMAKGLVIEAKLDRGRGPIATVLVQSGTLHVGDAFVVGNFSGRVRAL
                     VTDTGKKTTEAGPSVPVEVIGLPGVPSAGDAFTIVKDERVAREIAQERAMKQRAADLA
                     GPAKVSLDDLFAKIQEGNVKELPIVIKADVQGSSEALAAAVEKMPAGAVKLRVMHTGV
                     GGITETDILLAAASKAIVIGFNIRPEPKAAALAEREGVDVRLYSIIYDALNDIRAAME
                     GLLEPTLKERILGRAEVRQMFTIPKAGLVAGCYVVDGVISRASVGVRVIRDSVVVYEG
                     KLGSLRRFKDDVREVQQGYECGITVENFNDLKAGDIIEAFAIDKVAAKLESAHRGTSP
                     QPHRA"
     gene            complement(62251..63390)
                     /gene="nusA"
                     /locus_tag="PK589_01340"
     CDS             complement(62251..63390)
                     /gene="nusA"
                     /locus_tag="PK589_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNJ18263.1"
                     /translation="MNRELIAVIDEIGRQKGIDKARVIGAIESALQTAAKKRFGQAEN
                     IQVEIDSKTGEISVVSKKIIVDTVANPKAEISLKEAREYDEGAEVGDEIGSLIEMDEL
                     GRIAAQTAKQVIFQKVREAEWEAVQKEYSTRQGDLVNGIILGMERRNFLVDLGKTEAI
                     LPIQEQIPRETYRRGDRVKALLLEVRRTPKDVQVILSRSHPQFVAKMFELEVPEVGEK
                     IVEIKSIVREPGDRTKIAVSSRDKAVDPVGACVGIKGSRVQAVVRELRGEKIDIITWT
                     QDPRVFIAEALNPATIEKVGIDEEKKSALVVVADSQLSLAIGKNGQNVRLAARLTGWK
                     IDIISATEYEKEKAERDRDIKAALAEETEAQRQQDEARAAAQQAE"
     gene            complement(63432..63953)
                     /gene="rimP"
                     /locus_tag="PK589_01345"
     CDS             complement(63432..63953)
                     /gene="rimP"
                     /locus_tag="PK589_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNJ18264.1"
                     /translation="MSDAGAVSAGKPLVKRAEALNLRVQEVAAPILQSHGLELVEAVC
                     VGQGPRTVIRVFIDKPGGISLTDCEQAHRSLSPALDVIDPFPHAYTLEISSPGLDRPL
                     RTAHDYRRLIGEPVTLKLREPIQSQWRLVGTVLEVEDGAVTLAIQQKKTTETIRVEFD
                     HIAVARRKVEFSK"
     gene            complement(64043..64882)
                     /gene="dat"
                     /locus_tag="PK589_01350"
     CDS             complement(64043..64882)
                     /gene="dat"
                     /locus_tag="PK589_01350"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HNJ18265.1"
                     /translation="MPDIACVNGCFGPVAEAVVSIEDRGFQFGDGVYEVIRTYRGKPF
                     ALDEHLARFERSAQALHLSLGLTKDQWMALVQEGLRLSQFPETKIYLQVTRGQAPRDH
                     LFPASTVPTTVLTFRELHPLDASIRKAGVGAVTVDDIRWGRCDIKSVNLLANVLARQR
                     AKEAGVFEAILVRDGLVTEGSVSNVMVVRNGVVETAPEGHRILSGVTRAMVLELARKE
                     GLPVVETFVGREDLLAASEVFLTGTTVEVLPVVRIDGQPIGAGVPGPLSQRLSRRWEA
                     LVG"
     gene            complement(64907..65239)
                     /locus_tag="PK589_01355"
     CDS             complement(64907..65239)
                     /locus_tag="PK589_01355"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNJ18266.1"
                     /translation="MSPLSKAVPTGTEQEPGAKVVKARVGLPVHIRLWEDRTRGELWV
                     PIYDAAVMPLVEDDFLRTASNNAVDQGQRSFEFKPSVAGTHRVVFEKRLGWKFTAEDR
                     QIFYVTAS"
     gene            complement(65275..65592)
                     /locus_tag="PK589_01360"
     CDS             complement(65275..65592)
                     /locus_tag="PK589_01360"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNJ18267.1"
                     /translation="MEGFQCVAKVEDIPPGQVKVVKVNERAIAVFNIEGHFHAIYNSC
                     PHEGGPLNKGRVKGHVVSCPWHDLAFDVRNGQGTDGGGYCVGSYDVRVEDGQIFVGGR
                     RKV"
     gene            complement(65617..66114)
                     /locus_tag="PK589_01365"
     CDS             complement(65617..66114)
                     /locus_tag="PK589_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNJ18268.1"
                     /translation="MVTRASGSESEQRRKKIVTFVLLVILLISVSLFLGGPKESELII
                     VEFPNGKTMETEVASTPEKLLFGLAFREGLPADTGMLYIFESTGLHRVATRQFRIPVD
                     MLWIDESHHVVHILEQVQPCLQDPCPFYGPPPEPVRYLIEAEAGYAKRAGITIGMELK
                     FTLRM"
     gene            66362..66709
                     /locus_tag="PK589_01370"
     CDS             66362..66709
                     /locus_tag="PK589_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNJ18269.1"
                     /translation="MALLITDECISCGACLPECPNEAIFETRSDAEGKGNHVGDGQGV
                     GDNIYVITHDRCTECVGHFDEPQCAAVCPVDNCCIADPAYPETTDVLLEKAKSLNPDK
                     EIDPAKVWSGVRN"
     gene            66922..67257
                     /locus_tag="PK589_01375"
     CDS             66922..67257
                     /locus_tag="PK589_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18270.1"
                     /translation="MNCFTRLACIAILALAPLPSYAMTEVGDIGTIIESFITKQFPDA
                     ASHFWVVNDTQWDGDEMIVDMNTVVTDRREAPPTESRFLLLIVEGRLQGIQNIPLDAA
                     AECQKEQEV"
     gene            complement(67352..67831)
                     /locus_tag="PK589_01380"
     CDS             complement(67352..67831)
                     /locus_tag="PK589_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18271.1"
                     /translation="MGKTMVAVFFGLVMLMAGASGAYALQAGGVEIGDLDTGSVVGQP
                     FKELIVDAELYAVEIGNLKAWYPPVTVIDFKVRPGRPVVLKVTNNSSSEHGFQMTDPV
                     NAASPFVMKAEVVLKPGETKYIGVPTSDMFNATQGNTLTYRCHLHPAHVGGKLVMIK"
     gene            complement(68106..69680)
                     /locus_tag="PK589_01385"
     CDS             complement(68106..69680)
                     /locus_tag="PK589_01385"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNJ18272.1"
                     /translation="MMAQRPAVVPIPRLFLLFTALVTGAIVMALEILGSRLLAPVFGN
                     SLFVWGALIGIILAAMSSGYAFGGWASDRYRVAAVLAWLLLGSGAWTLLMSWMGQGAI
                     FQVAKLVEDPRWGPSLAACVLLAPPAFGLSGVMPALLRLAVVDMGYLGRHTGSMIALS
                     TVGSLAGTWGTAFFLLSWLGTQTLVATLGVIQLLLGLLWLQQGSGRVTKLTGVVMTGL
                     LISGWQLFGQAPPVAGLVYQEDSPYQQVRVRDDDLFRYLVLDRTWHAVMWRSDPTALF
                     LPYSQLMVAAVALAPEPKRGLILGHGGGSLAKWLAQVWPELELDVVEFDPVVVRMAEQ
                     YFDYRPPANHHVSVKDARVFLRDTKATYDVIWVDAFARHLIPFHLTTVEFFSELRGRL
                     NPNGVLALNLASSGEGGDLQRANAVVQTLKSSFPSIESFGVKGPWRAHQTTAENLIFF
                     GGGPIATMSYDEIVKRIYMQVEARRLPLEAIALLASRRATPWPPGLTLTDDYTPYDLL
                     IGSHAVEMSPEGNSSP"
     gene            complement(69744..70154)
                     /locus_tag="PK589_01390"
     CDS             complement(69744..70154)
                     /locus_tag="PK589_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ18273.1"
                     /translation="MVVAGGPIQRPLAMMMRPITKTVRPDDSLLVVAQQLRDARVGAM
                     LVADHGDYVGIVSEADLVRKAMASGAASDQVTVRSVMSAPLMTIDIAQSAHEASDVMA
                     ERGIRHLVITDEGRVVGMISVRDLLRYFKNWGTL"
     gene            70352..71671
                     /locus_tag="PK589_01395"
     CDS             70352..71671
                     /locus_tag="PK589_01395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNJ18274.1"
                     /translation="MHISVIGTGYVGLVTGACFAEFGVNVTCMDTDARRIAKLEKGEV
                     PFFEPGITELVAKGIKEDRLHFTTDVAKAVDKALVIFIAVGTPPKSDGSADLSYVEEV
                     GRGIAKNMTGYKVIVTKSTVPVGTGEKLREVIKANQTGRFRFDIVSNPEFLREGSAIE
                     DFMRPNRVVIGADSEQAVAIMKDLYRPLYLLETPIVVTDIPTAEMIKYASNAFLAVKI
                     SFINEIATVCEKVGADVQLVSKGMGLDNRIGNKFLHAGPGFGGSCFPKDLAALVQTGE
                     RVGYPFQIAGAAAKVNYEQHLRMVQKVKDACGGVKGKTLGVLGLSFKPNTNDMREAPS
                     LTILSELMKEGAKIRAYDPASMEESMKLLPGMVPCQDTYEVAEGADGLIIMTEWNQFR
                     NLDFERLKKAMREPLLLDLRNTYESDRVVPFGFRHVSVGRPTKNPAA"
     gene            complement(71693..72403)
                     /locus_tag="PK589_01400"
     CDS             complement(71693..72403)
                     /locus_tag="PK589_01400"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ18275.1"
                     /translation="MAEEAPVKLIQIGPKGGPKKDGFNLVTERVVAVNPEARQLEVEL
                     LAYDGKTVVLDVGEEALEDFLKIKPGDGATIRVVEEGGKRVAKSFRIRAKDPNAAKAD
                     AMLIDLKDSHWLNRKYAAEVLGDLKDPRAVVPLVEALTDEVGDVRQRAYDSLIKIGGS
                     AVPSLVPLLASEEDDVRQSATEIIRKIGKPAVEPLATALAEADDRLKTKIMKVLDRMG
                     YKPKAKEGAQPEPAKLLS"
     gene            complement(72424..73185)
                     /locus_tag="PK589_01405"
     CDS             complement(72424..73185)
                     /locus_tag="PK589_01405"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ18276.1"
                     /translation="MSDAERIAQLIAALRDDNEALRDHAMASLGQMGIEAVPQLIGLM
                     ADEDVVIREAAATAVVRIGPIAFDQLLEALRDDEWAIREQAANVLGRFRDPRAIDPLM
                     VALKDKDGAVRTAAVWALERIGDSRATPGLIEALGDGTVREDVARVLKKIGDTRAVDA
                     LIGGLLGPNWMVRRHAAEALGKIGDPRSADALIQSLQDEDWLVRRNAAESLARLGAKH
                     AIEPLLPLLEDENTMVQETVEGVLASLGWKQATSS"
     gene            complement(73178..74278)
                     /locus_tag="PK589_01410"
     CDS             complement(73178..74278)
                     /locus_tag="PK589_01410"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ18277.1"
                     /translation="MSKESIDTLVSELTHEEEWRRMRATAACLAGGPRAVQALTHALQ
                     SGDVPLRVEAAAMLSRIKDPAAGVALVTLIRDPEATVRQAAFAALEQMAGNLDDETAA
                     GLVRNLHESPDEEVRHRVRQLLGVIPNAITPLCEMLRHPDAEAQMTAATILEHLLDPR
                     SADGLIDAMASPAVREIVVRTLKKLSAVRERIDATFNALRDVEGASEREEARMATVMD
                     LLGIGRPSVEILIEYLEDDDWLIREAAADLLGKIGDVRAVEPLMKRLQKDKDTGVKEY
                     AVKSLGLIGDPRPAQLYIEAIPIRPLRVMAIEALAKIKDVEVLRPYNELFNRLRSDRD
                     GIVSYSAGLIADKLAALEGEQPVGQEESDDHE"
     gene            complement(74373..75455)
                     /locus_tag="PK589_01415"
     CDS             complement(74373..75455)
                     /locus_tag="PK589_01415"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ18278.1"
                     /translation="MADTVSEQIAALSDEDWAVREEAATLLGSLNDARAVLPLTHALR
                     DTDRAVREAAVGALSSLGSVSVPALAGCLADPALQVQEAASAILASLADARVLAPLMQ
                     ALGSRDWIVRMHAAKGLGRIGDPEAVSALMPLLQDKVKAVREEASTALAAIGVAAMAG
                     LIEALQHDDWLVRLHAVEALGKLKSPDSVEPLLRALFNERDSAIREDVVRALGAIRDG
                     RAVDYLVTVMKEPGLRLLAVEALGHIGDRRVVPILRRVVEGVPLGEPRDSATPCADGW
                     TDEMATMGMAARALGMIADAVAIPSLIVALRNTITRSEAAAALTKFGPAVIPSLLPML
                     AKEQDENVRYHVRETLTAVGWRAGRV"
     gene            complement(75636..77006)
                     /locus_tag="PK589_01420"
     CDS             complement(75636..77006)
                     /locus_tag="PK589_01420"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ18279.1"
                     /translation="MAQALDDLLDSLEDPDDATREEAAKALAQLANPTTLDALIGACG
                     DEYWSVRTRAGWGVAKIGGPKAIEALIALFNDPIMEVRNEAVAAVVSLGVGHLDRLLA
                     AMKDERWRVREHAAKACGDLRDARAVDALIFACRDRDGAVKSAAAEALGKIGDAKAIP
                     ALIKLFRDSSKIVRETAGIALVAIGQPSVDLLLETMKDKDFVVRCHAARALGGMTTDY
                     QIGRSWVREPRVVDALIEALKDPDRAVREDATIALGMIGDPRAIDGLLEAMKDGAVKR
                     HAIASLGMIGDPRALPAVLAALKGKGVRQDGTPTPGCIVSEDAFIKEAAATALGHFRD
                     PRVIPDLIMLLKDGVLREKAAAALVLIGDSAIEPLISFLYDPKASEVEAEGERVLSYA
                     SVRLTAKDSLRLLVVETLEHLGWTPPDEETSVDSSQADNLRVDRPLGDIGRFGPSGDF
                     AKGSVR"
     gene            complement(76987..77478)
                     /locus_tag="PK589_01425"
     CDS             complement(76987..77478)
                     /locus_tag="PK589_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18280.1"
                     /translation="MDILSHRLFTGRQTLIRIVALSCAVFAGHAERLLAAGPPPAYTK
                     EHTITIDPTPLVPQSWWQVPGITPPLWVLDPETSDAYRTTETRELHLKPGQYKFISFT
                     FDFPFTVSLDGKLEYSKSLDQCVEGRGTQTLVVRCKRTYPHGGQRDAYYGNSEVGDGT
                     GTR"
     gene            complement(77486..78535)
                     /locus_tag="PK589_01430"
     CDS             complement(77486..78535)
                     /locus_tag="PK589_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNJ18281.1"
                     /translation="MSFIRNISTVPVAFCLAVVLSMTVVASSSSSVALAARGAGDIPP
                     SVPAGMTEHVILFVLEGFGQESLKSGVMPVLSSLVKEGAVTWSATAVAPARRLPTMAS
                     LVTGMPVAKHGITWNVFEFSRGYPRAPTVFDYLDLSGGRDSAIFYMDESLYQLAKPEP
                     YTDYQMCGPLRAECNPDRLVGYVRDYFKKATSGSGYGHAIPSLPHLLVVHLPAPGRVG
                     EAQGWKSAAYKDALKAVDKAMGTVLDLYRELGLMKRTTVFATSLSAFGETQFSSGDMS
                     ADQAGAVPVVPWIASGVGIKAGYAIRQPVSIIDTGATVMRALGLTTYTEWESHPVEEI
                     FKTAFTVAPVNPLLQ"
     gene            78900..79997
                     /locus_tag="PK589_01435"
     CDS             78900..79997
                     /locus_tag="PK589_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18282.1"
                     /translation="MTDWETRVDAIRLAAEQAKQQALEQVASYLESVTVVQNLDVTQD
                     ELRSYTAGMVLVLDQQVDTALDGDVVVIRVTLKAQVDSEEAAQAILAMKQNEDVRQEL
                     LALKEDVESLHKQLEDANQALAHANSPEQVRKLNQQRTDLLNHAESDAMVAQAWTDWL
                     LIAPFAHPYPSAGVAQVQALLGIAGRLNPTNPHLAIAQQTVGAKLPPAPPHPRQSPVP
                     HTVPFLPGYYVVPKEPAPPGTPPPTDSKQLSSVYQLNPLLPNPSGQPPQSGNSTTIIQ
                     AHPRPGTSPSVPDRNMMPTYRQIPAPNSQASHPTLHTYRAGGSPPSASIPGSADGGIR
                     GGRPPVIHQTKPLSPSESTAERGSATNQGDK"
     gene            80000..80545
                     /locus_tag="PK589_01440"
     CDS             80000..80545
                     /locus_tag="PK589_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HNJ18283.1"
                     /translation="MPLTSSHILMAVGMAGTLLVATATMAGELTPQDVRTHADQHFQQ
                     LRAREQTIAPTTRDRPQQDPSSSYHPDQFFIGQGQGDLSKGKLVCQRVSELAARADIA
                     KQIRVQVKEHATDRLRERTGREPEQDIEVVREEIVQEYLQGVTIVDRRIDDEAKTCTA
                     VAVMPKATLNTSLPAESSARH"
     gene            80615..81868
                     /locus_tag="PK589_01445"
     CDS             80615..81868
                     /locus_tag="PK589_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18284.1"
                     /translation="MPIENNFQHDELSRKNPGDRLTYADWTPPPDMDSPAWQAAMGEA
                     GQKLSQAGVRVIVFLHGAIPGTDIFGLGRLDEVGGLKRGYSRGISGLDSLLSFMREGT
                     NGITPLPGGMKPPLANDEATKSLLDSQIGDAGNFTAPIVERCRKALNQHVSKPIVCVR
                     ELWSSEHHHLGRALAACRLLDRLRALVTEHHLGTGDRILIQAHGQSGLVLALAANLLC
                     PSPITGRKAFFDLLLAANPQHSDVQAIQRIDPLLTSGSLLNGAALDIVTFGTPVRYGW
                     DPSGIGKLLHIVNHRNLRTDGKTWLSKMELPQISVEMPIAWGGDYVQELAVAGSDALP
                     AESAKAANKAVWELLEPYDGFERWVECARRATRFPSEGQCLLVDYKDCTSSTNVRDHY
                     YGHAAYTRANAILFNFNQIVGRLYS"
     gene            complement(81889..82728)
                     /locus_tag="PK589_01450"
     CDS             complement(81889..82728)
                     /locus_tag="PK589_01450"
                     /inference="COORDINATES: protein motif:HMM:NF037404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiV family abortive infection protein"
                     /protein_id="HNJ18285.1"
                     /translation="MTAAKTFRLAAVAALTNANQLCAEARLLAENEHNSRAAALAVIG
                     LEEFAKAVACTVAAIFPEKSSDLGNRLNAHNVKQWIYQTFEGIQIQVQDWLNVVPYES
                     GAGSPIEPETILLETFRTLSKSRWGSVVPTKNSADSAAKQDQKYLNDPRMHARPTEIM
                     TTPFIKNVAFYVDVKKGEVLLPNRVDRYANYEIHGLTWSLEHSSPLKQILSDDHKWEA
                     FADAVRRTPGAGLSIEAEVLTPKKTAIKQLRRLPETATWDQIKKEIERGAQEATEERK
                     LTS"
     gene            complement(82819..83205)
                     /locus_tag="PK589_01455"
     CDS             complement(82819..83205)
                     /locus_tag="PK589_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNJ18286.1"
                     /translation="MSPRKRRLTIPEPEFQALVQQALDGLPDEYAKRITNVAVVVEDE
                     PSPEVLADLDMDEDEDLLGLYQGLPIDKESFFQAGGQLPAKISIYRGPILRLCRTKQE
                     VVQEVRDTVVHEIGHHFGFDDDEMPY"
     gene            complement(83378..86023)
                     /gene="polA"
                     /locus_tag="PK589_01460"
     CDS             complement(83378..86023)
                     /gene="polA"
                     /locus_tag="PK589_01460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNJ18287.1"
                     /translation="MPTLYLIDGSAYIYRAFFALPPLSNSKGLQTNAVYGFTTMLMKV
                     LRDHHPDYVAVVFDEKGPTHRHEAFKEYKAQRPPMPQGMSAQVPYIHRVVEALSLPVI
                     RQAGYEADDLIGTLARKGEGEGLDVVIVTSDKDMFQLLTPKTRIYDPVKDKWFGEADS
                     QVRFGVEPARVVEIMGLMGDTSDNIPGVKGIGEKTAVKLITQFGTIEELLRRVEEVTP
                     AKTKNLLLEQGESARMSRQLATIQVDCPIEFAPAHFQARAPQTEVLVSLLRELEFMTL
                     AKAFQGDTPEQNRLGAEVQRIHDTAGAEDFLKRRQPDTMMGLACVLSDEAGVRADIQG
                     CAFGWPDGTVAFVQGEAQDWPQPLTAYVRDGSRPKAVQDLKPTLLALQRQGIDMPGPY
                     FDTMVADYLLNPNRRAHTLEAIAMDLLSYQLGTGEREEGGKEPQSLFDVDERVVRRTG
                     EAAAVTAKVAPMLRDRLKEQGSLSLFEQVEMPLVPVLADIERNGFLLDVEGLRTLSKE
                     LERELEQMVGTIYRLGGGEFNIGSPKQLATVLFETLGLKPLRKTKTGFSTDEDTLTQL
                     ANQHELPAQILSYRTLTKLKSTYVDALPQLVNPDTKRLHTSLNQTVAATGRLSSTDPN
                     LQNIPVKGDYGLRIREAFIAPPGHHLLCADYSQVEPRILAHLSQDPRLLEVFEKGEDI
                     HMATAMEIFNLPAGDVTREMRRAAKSVVFGIVYGISPFGLASNIGVPQAEAKKYIETF
                     FEKFAAVRALMDHNINDGKTKGYTTTILGRRRPIPELQSGDPSQRGVGERMAVNSPIQ
                     GSAADLIKLAMIKVHRRLRDELPRCKMILQVHDELIFEVPDQELEQAKQLVKAEMEAT
                     GDALGLSVPLKVDVGVGCNWRAAHP"
     gene            complement(86087..86515)
                     /locus_tag="PK589_01465"
     CDS             complement(86087..86515)
                     /locus_tag="PK589_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HNJ18288.1"
                     /translation="MSDMLAQIESYVPASVLIWFAVSSVVMFVGTLIAIPVILMRLPA
                     DYFDTRIPRPWMENHHPVLRLLGHVVKNVVGAIFLFAGFLMLFLPGQGVLTMLIGLSL
                     IEFPGKRRVEAKIVGQSTVLSTINAMRAKFDKPPLIIAPE"
     gene            86639..87007
                     /locus_tag="PK589_01470"
     CDS             86639..87007
                     /locus_tag="PK589_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18289.1"
                     /translation="MLRTIRSSACRLAVLGLILVLNTVEVFDQGAQAADSAAPPELQI
                     DISKIGLGKEVVITQGNKEWFMRVEVTPENSVVVRQEKQGERYLLDESEMHDRAMTAA
                     EVDAAISDFINSVKTRQKVK"
     gene            87013..87432
                     /locus_tag="PK589_01475"
     CDS             87013..87432
                     /locus_tag="PK589_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18290.1"
                     /translation="MADKPKFVLFAHNATYDKLHQVATLGLTASAMGKDVIVVLLFWT
                     IKKLAEGKIDHIDFPPEYAASAEEVARLLKEKKVPRISEMFHEARLVGQFRLIACSAG
                     LEYMGVEAGAVERNVDEVLGLPAILSLTAQAETKLFI"
     gene            complement(87491..88150)
                     /gene="nth"
                     /locus_tag="PK589_01480"
     CDS             complement(87491..88150)
                     /gene="nth"
                     /locus_tag="PK589_01480"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNJ18291.1"
                     /translation="MQAHEIHAVIRTVKREIARWPDPVVGVVAKKSGRDPFLVLISCL
                     LSLRTKDKTTAEASARLFALASTPATMQQLSQSTVESAIYPVGFYRTKAKQIQQICRQ
                     LLDRYHGRVPDQIEALLTLPGVGRKTANLVVTVGYEKPGICVDIHVHRISNRWGYVKT
                     KTPDETEKALRGKLPRKYWITFNDLLVPFGQNLCQPVSPFCSRCKIAEYCDRVGVTKS
                     R"
     gene            complement(88220..88414)
                     /gene="rpsU"
                     /locus_tag="PK589_01485"
     CDS             complement(88220..88414)
                     /gene="rpsU"
                     /locus_tag="PK589_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNJ18292.1"
                     /translation="MEIKVFNNNVEKALKVAKKKLAGEGLFRELKRRRYYEKPSVRKK
                     AKEREAQRRRQKWLAKRRPE"
     gene            complement(88438..90000)
                     /gene="ftcD"
                     /locus_tag="PK589_01490"
     CDS             complement(88438..90000)
                     /gene="ftcD"
                     /locus_tag="PK589_01490"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNJ18293.1"
                     /translation="MSQVIECVPNFSEGRNPDVIQALVALVRSVPGVALLDESHDTDH
                     HRSVVTFAGRPYAVAEVAFQMARLASQLIDLRSHHGEHPRVGATDVMPFVPIREVSMQ
                     DCVQLARMVGQRIGNELKIPVFLYEQAAARPERVQLEWIRNGGLKGLADRMAADPAWV
                     PDFGPKQLHPTAGATVVGARWPLIAFNVNLKSGDLLVAKAIAKAVRQSSGGLPSVKAI
                     GVELRSQGLVQVSMNLTNYEETSLQVAFTAVEREAAARGVEVAGTEIIGLVPEQCLID
                     TAQRALRLDRFEGRQVLEMRLDSAESRAAIGRLAGSQPVKESPSLPAKPVASTNVLAA
                     EQGLTGGSVGALAASFAASLGTMVAKLNRARPAETRLTEIGTRFRELAQADRDAYARV
                     LKARKLPPNHPDRAVQIASGLLDAIETPMEIVKLCCEVIPLLRGLLAQAKPELQPDLK
                     MGIELADAAINGCVAMVEENMKVQPNQLLVASIRERFAVAEQMLVDAKSLCYTPPFDS
                     WSQNMLNILKLR"
     gene            complement(90138..90383)
                     /locus_tag="PK589_01495"
     CDS             complement(90138..90383)
                     /locus_tag="PK589_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNJ18294.1"
                     /translation="MIQADVKLDTLGYFCPMPIILTSKKIKELTMGQVLEVVSDDEGI
                     KKDMPAWCETTGHQMVGLEEEQSSSKRIYKAFVKKAK"
     gene            complement(90402..90932)
                     /locus_tag="PK589_01500"
     CDS             complement(90402..90932)
                     /locus_tag="PK589_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNJ18295.1"
                     /translation="MTTAQIEPTAALAELRESKADRVTIVLLSGDLDKAMAAFIIATG
                     AAAMGMRVTVFFTFWGLNTIRKKGASSSASDWLRRMFGFLNKGGADTLPLSRFHFWGL
                     GTRMMQVVMKQNRMPGVPELMQMAIDLDVRFIACTTTMGLMGITKDTLIDGIDQFAGV
                     TTYLAEAKQGSVNLFI"
     gene            91158..>92013
                     /locus_tag="PK589_01505"
     CDS             91158..>92013
                     /locus_tag="PK589_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNJ18296.1"
                     /translation="MSTDLITELKPTKSAPLFGFWYPAATSSSLGVGQMQTQVMLGLP
                     ILICRDRQGQVAAMRDICPHRGMPLSFGHFDGERVECAYHGWQFDTGGRCRHIPALVE
                     GSPLQAEKIGITSYPCRDEDGYVWVFLPDPQQPNQPVPELPRLPLPSAPYRMVQISTI
                     LDCTIDDGIVGLMDPAHGPFVHQSSWWRTQASMHDKAKTFEPIPNGFRMVPHAPSKNS
                     GPYKLLNKLYGGPLTTTIDFVLPNQRFEFVQCGSMFVSSRATVTPVGEQQCRIDFTAA
                     WNILPWLPF"
     assembly_gap    92014..92113
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <92114..92401
                     /locus_tag="PK589_01510"
     CDS             <92114..92401
                     /locus_tag="PK589_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNJ18297.1"
                     /translation="QCRIDFTAAWNILPWLPFAKPLFRYFANIFMKQDKQAMERQAVG
                     LKYKPALMLIDDADTPAKWYYKLKAAHLASVQTGRPLEHPLKGPVTLRWRS"
     gene            complement(92460..94484)
                     /locus_tag="PK589_01515"
     CDS             complement(92460..94484)
                     /locus_tag="PK589_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNJ18298.1"
                     /translation="MSDYAVLGDLLVIYAVSTAVVFVFHQFRLPSIAGFLVAGALIGP
                     HGLHLIPDVSQVQVLAEIGIVLLLFTIGMEFSSAHFASARRILAVAAPVQTGGVLILA
                     LLGALAVGLSYQQGIFWGFLLSLSSTAIVLKALSEQGESDSFHGRATVAILIFQDLAV
                     VPMMLITPILATPSGSAMGMVLLTLLKAAVVVVLIVAAAWYLVPRLLRHIVRSRSREL
                     FLLSIIVLCLGIAWLTSLGGLSLALGAFIAGLVMSESEYSHQALAEVLPFRDSFNSLF
                     FVSIGVLMDLRVVLDHPFIVLGLLVAVIAGKLLAGAGAMVASGAPPRSAVLVGVALAQ
                     VGEFSFILAQQGQEAGLFSGDHYQLFLAVSVLTMVVTPFVMQWSPHLGRRIEALQRVR
                     GWMPTRTVAHVEQLEGGQVRIKDHVIIVGYGLNGRNLARVLSETEIPHVALDLDGETV
                     RRESRHGVPIYYGDGSNANVLRHMKIEDAKVLVIALSDPFTARRTVKVAKGLNAKLHI
                     VVRTRYLRELEELHQLGADDVVPEEFETSIEIFALVLRTYCLPQEFVARKAEQIRREG
                     YALLRRSEMPELAHHLRGGTLTDVEVETCRVDEDAPAVGKSLTELSIRPRTGASVIAW
                     TRSQVTQSNPSETVRLQAGDVVTLLGSREQIRRAMALLNEPNHGTSHQLT"
     gene            94652..95335
                     /locus_tag="PK589_01520"
     CDS             94652..95335
                     /locus_tag="PK589_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNJ18299.1"
                     /translation="MRAIIFDFNGVIADDEMTHLECFQKALREAGLVLSKEDYYGTYL
                     GMDERTCAAAMLTRRDGTCDHTTLARIIERKAALFRTHTATHKPALFPWVTGFVQRAV
                     EQYRLAIASGGRREQIQSALAGHSIEATFEVMISADDCAVGKPDPAIYECALRQLAAK
                     RPKPPLLRADECLVIEDSRAGIQAGLRAGMRVLALATTYPASQLTEAHLVLPSLEGID
                     PSELARRLF"
     gene            complement(95384..>95617)
                     /locus_tag="PK589_01525"
     CDS             complement(95384..>95617)
                     /locus_tag="PK589_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18300.1"
                     /translation="EQRADLFRQSPVLSGRLRVSEQTLIPYIGAGFGGGYVTERDRAL
                     NPQPTLPQQNLFGEAMGKSMMPNEFQMGIRIPF"
BASE COUNT        20450 a        29420 c        25144 g        20103 t
ORIGIN      
        1 tcaactcgac gatggcatcg ttcactgctt gaatcgcatc cgcgacctgg gcctgtgcag
       61 tgacaggctg gctgccgatc gccaacgctg cgacgcccag cgttacgaac atgagtttct
      121 tcattgcgtg tctcctttgg tgaaagccat cagctatcag caaggacgct gtcagctgaa
      181 agctgtaacc tgatcgcttt ttcctagaag ttcatccaca gcgacatata ggcccagtgc
      241 tgattgacct gttgatttaa cgtgctggtc aaatagccac cgctgaagat cgtgccatat
      301 gcagcctgca gggcaacttt gccgtcggcg aacatccggg tgtacgccac gtcgatttcg
      361 tcgccgatgt gggtcttcgt gttgttggcg ttcgagaaca cgtaacaccc ctgcgaggct
      421 cgataccaac agtccttcga attggccagg ttcaggttcg tgtaccacaa ttcgatgtga
      481 tcacgcgccg aaggccgagc ctgcaagttg actgaaggag acagcatgtt cttccacgct
      541 tgcacgtcca tgtaacccat gtggatgtgg ttcgtcggga agaagttttc aaaggtgttg
      601 gctgtcttac aattggtccc gttggtgcag gtgttgttcc ggctgtcgcc agacgcgtaa
      661 tcgaggttga aggccaggcg ggtcttccac gccgtgtcat agaacgtgta gccaatccag
      721 tttctggtcg cccacgcatt gatatgcaaa catttctggt cgcccacaca agcgccagct
      781 tgccccatct gaccgaactg ccaggcaact tcattgctga agtcgaatcc acccttcctc
      841 atttcaattc gattaccaat catatgccga gtctggttgg catgctttgc ggtacccagc
      901 ccaaatgcct ggtttccgac ccctgccgta gccgatgaac cgtaattgtt tttgtaatac
      961 acgtagaacg gctcaatgac cattccagga atcattttca gctgattgta gaaaacgatc
     1021 atgtccgcat cacgattggc atcgctgccg cttgcgttgc cggcaatgtt gccgcttcca
     1081 ctgcctacag cagcagcctg attgaggtct gattcggaat tacggaacca accggcatag
     1141 atgtcccagt tcttgattcc ggtgtaggag aacatcacgc catcatgtga gtagccggtg
     1201 ttcgcccagt cgaagtggcc gaacaatgag tggttaccga agactacata ttgtcgaccg
     1261 accttcatgc tcaagccctg cacatcggcc agatttctca ccaacatgta ggcggcacga
     1321 acacccaatc ggcaagcacc ggttgctgtc gccgagcaat tgtggttaag cggatctccg
     1381 ccgttgcctg cgttagtcgc cgtaccgttg ccaccccacg tggcactgtc aatgatttcc
     1441 acgtagaagt tcacatcggg cgagagatca taaccgatac ccaagcgcat ccattgctgg
     1501 acaaagaaat cgttgccttt gttaccacca gccccagccg caccacgcgt tccgaacgtg
     1561 ttacaggtcc cgttggcatt catctgcagg ccaaagcacc cgttgtttcg catttccgga
     1621 cgcacacgca gatcggcgcg catccacaga ttcttgatgt cgaaattccg gccgatgacc
     1681 ggatcgtaca gttcgtacgc ttctttctgc ggcatgttgc gaggaattgc ggggagattc
     1741 gtgatgcgtt cgcccggggg aagttcgaat ccggcaaagg ccgggagcgt aaggcactgc
     1801 agagctgaga ttgccgcagc gccgaagatg gcggccaacc ggccccatct ggtccgtcca
     1861 tggatgcgtt tcatagcgtt cctcctgtga attggatggc aacccttcgt gtacccattc
     1921 actctgggtg ccgcctaaaa tccaaccgct attgcactac aaagctaact cgtatgtggg
     1981 ggcctcagtc agccgaacca gaatggtgcc caccattcgg tcatgacctg cttcaatccc
     2041 gaagcgagtc cctcacactg tcagtcttgt attgttgctg tggtcacctc ccttcacagc
     2101 cgtagagcga atatgaaatg gtattaggcc tgcttgggca aacccgattc ctctgcccca
     2161 tgctctacgc ccgatgttgc gtggttgatc tgttcttctg cttctttcat cgacgactcg
     2221 aaatcttgtt ccaccatctt catttcctgc tggatcatgt tgagttcggg ctcaaccgtc
     2281 ttcttcaggt cgtccgccgt ctctttgaac cccttgacgg ccttgcccac ctgacgcccg
     2341 atttcgggca actgcttagg cccgaacaac aggaacgcga tgaccaggat gatgagaatt
     2401 tctccagcgc caagaccgaa catggtgttc ctactgaggt ggttgtgtga aagaccaggc
     2461 agcgcgaccg tgaaagacaa tggccgtcac ccgggtctga tcctcagtca cgcaagttac
     2521 ccctgcgtcg tccgtggcgg tggagccgca ccggcctgct gcgcaggcgg cggtggggcc
     2581 gactgtccgg tttgctggat ttgtgccgtc ggctgagccg cggctggttc cgtctcgttt
     2641 gccgtcacat caatggcatc cgcttcgtga accgatttct tgaacccctt gattgcttta
     2701 cctaatccct caccgagctg gggaattttc ccggctccga agatgatgag cacgatgatg
     2761 agaatcagca gcagctccat ccagccgaac gaaccaaaca tggtactgta tgctccttgt
     2821 tacagctgtg cgattattcg cacgacatat gttcacgcac cggcgactcg actgccactc
     2881 gtgaagtacg tcgtctgaga agatcttgcc tgcgaacccc atctcctctg aattttcccc
     2941 acgcacggag aaggaggggg tcagtatcgg aaatcgcggg aaaggctata gaaacgactg
     3001 gaattagtca agctctttct cctacaggtg cccccttacg tctctttccc tcctgcccga
     3061 accagcgact acggtcggaa cgcgaaatga tagtactcaa ccggactcgg aggcgctcca
     3121 agcgccatca accaggcctc ccgctcgtat ccgagctgtt ccaaatcacc ctgtgcctga
     3181 ctcaattctt cttcggtgac atacgcgatg gtgtcgggaa tgccgttggg tttcatcgag
     3241 aggagcgtgc cctgtgccgc ttcgcgttct tccgcaggca tttcgctgcg cagaggaaac
     3301 tcgatcttgc gttgatacgg gctctcgtaa tgttcattca gggtacggat cgttttcagc
     3361 gtgagctgat ccaattgcca gggctgaacc ttagagccca ccgtcggatg acaggccagg
     3421 agatccatca gcgtcatgac attggttccc agtcgacttc ccacaaactc acgctgggtt
     3481 tcaatgatgg tggtcgtgat cccgagatgc ttggctacgg cttgaatgag agccagattc
     3541 accatgaaac tgtattggcc gccgaactgg ccatagcagg gcttctcggg atcattgagc
     3601 acctgcatgt ccgctgttcc cgcatcgaaa ctgctgaacc ccacggcgtc cggggccagc
     3661 actcgcttcg cttccttgat cgacccgaag gcaccgagat tgaccggcac gagcacttct
     3721 tcatcaatcg ccttcataaa ctccgtcagg gttttgcgat aaggcacatc cttccaatcc
     3781 accttgtgat attcgcgctc ccagatgaga tcgtccagaa agggcgggaa ctgtttcaat
     3841 ttttcgatgt ccttggcgtc gaaggcgcgc acaaacgcgg accagtcggc aatcgctgcc
     3901 gccttgcgct cgttaaggtt cgggcggaga tgctcttcct catattcccc ccccttcttc
     3961 accacgagct tggtggccaa ttcattccac atctcattac agatgatccg gtcgaccgta
     4021 ccgtcggcga tggtcgacat attgtccaca tcggcacaga gggactcgac cttggagaga
     4081 tgcggaatca aatccggatg cgtgcgtgcc cgctccagag caggagcatg agaatccacg
     4141 agtacatacc gcacccgcgg atagatctgc cccgctttgt cgagcttctg tagatgactg
     4201 agaaagcagg ccgcgaggtt gccgttaccg gggccccatt ccatgacggt caaggatggt
     4261 cgggaagcgg catcgccgga ggcagaagac ttcgccgcag ccttcttgtc acgtttgact
     4321 gcctgctcga aatagtcggc cgccaacgca tgggccaacc gataatcggc cgaggcgaag
     4381 gtctgataga agctgcgttg ttgatcaccg cgcagctgat agaacagagt attgatgtgc
     4441 gcctgccagt aatcgaccgg cttgtattcc ccgatgggct gcggtaacgc gtcgggatcc
     4501 tcgacgggtg tcatgaggtg ctgttccatt ctgtccccct gtagaaagag ggaaagtcta
     4561 acaggaagca attttcaccc gcaacctctt ccggacgaat ccccctcagc attcatgatg
     4621 cctttcggtg aacgccccga ccgagttccg acgctgtatg ccttccttga caggcctccc
     4681 gggcggagtg taccaagaca ggatgagatc ggcgtgtatg ctcgtctgtc ccatccttct
     4741 tttctccggc tctctcttat gggccgaaga tcttcccttg actcgcaacg ttcccattcc
     4801 tgattttcaa aaccgcaccg agagcgccaa gccctacaat ttcgacgccc cgcccgaagg
     4861 catgtttcgt cacattgtgc tcgccgaagg gttcgatgaa gaattgggct tccagcggac
     4921 ccatgaaatt gttcctgtca aaccgaccga acgcttctcc gccggggcca gaccgatttt
     4981 tatcgtgttt gaactgcacc agcactacca atccttccag gtcttcgggc gctgctatcc
     5041 ggaagaggtg gccggagtcg atggaaaagt cgtagttgct gaagatgcga tgtacatcgc
     5101 gctggaagat caaaccggct acctgaaatt gttcccgaca gagagcggtt ggaaaccggg
     5161 gcggtataaa gtggaaatcc atgccggcga acagatcaac gacatgagcc tgatgggcac
     5221 catgcgcttc acgatcactg ccgcatctga atccgaagtc cccgccagaa gccggtgacc
     5281 aaacccgcac gcgtcagagc gattgacggc tgctctacca ctgggctata ccggtctgcc
     5341 gccgcaaatg ccaggcgcct gggccttctg gcgcctgccg gtcgaaagga acgtcgtcgc
     5401 cttgcagtgg gatattctgg ccaccatcct cctcacggca accattcagt cgctctttgg
     5461 tgtgggcgtg ttgctcttcg gcaccccgat tcttcttgtg ctcggttatg acttcatcac
     5521 cgcgctgaca attctgcttc ccatttcgct gaccatcaac ctcctgcaag tcagcaagga
     5581 ctatcggtat atccagtggc cgttctatcg gcagatcctc accctgtcta tcccctgcgt
     5641 ggtgctgtgc ctcttattgg tcacgacgct gaagctgaac atcggcagca tcgtaggcct
     5701 gtttttgatc gtcgcggcgc tgaagagcgt ctatgggccg ttcaacagat gcatcgaatc
     5761 gctggtgcga tatgagcgca tctacttcac ggtgatgggc atcgtccacg gcctgaccaa
     5821 tctgggcggc tcactgttga cagccatgat tcacagcaaa ccgtacgata aggatcagac
     5881 ccgggcgaca acggccgtct cctacggaac cttcgcgctg tttcagctca tcacgctgag
     5941 catgtccctc gggccttctg ccattgccac atccaccacg gcactctata tggctgtagg
     6001 cgtcagcatg ttcctggcta ccgaactctt gctctacagt aagatgagca cagaacgata
     6061 ccgcaccacg ttcgccgcat ttttgttcct gtccggcacg gccctgatct tcaagtctct
     6121 cttcatccga tgaccggcaa ggggtcattc gccagcggca ttgtcagtcg ccaggcctcg
     6181 tcgcgtatcc gaaggggctt tgtgttgacg ggaaacgggc tctcagtcta gactttgccc
     6241 agccgtatat tctctccggt gatggagttc accgagaacc gccgagtgcg atcctcgcct
     6301 cggctgataa ctcctaccag cgcccacgcg gtaggagttt gtttgtgaac cggtcccccg
     6361 caacgacacc accctatccc cagtatcagc tgaccgaggt ccttcgcgac attttcgccc
     6421 acctgggcat gttgctgagg ctggagcgat ccatcctggg catcatcgcc tcgtatgcag
     6481 tggccatcgg gtttttcctc ctctgcgtcc ccattgccgt ccaggagctc gtcagtactt
     6541 tctcctttgc gatggaacct cgcatgatct tcacgctggc cctgttcgtc gccagttcct
     6601 tgacgggcgt cgcggccttt cgcgtgctgc aggcgcgggc cgtggagacg ctgcaacagc
     6661 ggatctatac gcgcgtagcc atcgccttca cgcgccttct cccgcagctt cgcgacgaca
     6721 cctttgccac gccgcaagcc caccggttca tggaagccga tctgctgacg cgcgccctcg
     6781 tcgcgatggt cgcggatctc tttaacgtgg ctgtggtcgg caccatcggg atgaccatgc
     6841 tgatcctgtt tcatccctac ttcgtactct acattctggt gctgattctc gggtttgtgg
     6901 ggttactcac catctttggg cgggggggat tctttatcac cctagaaatg tcccgcttgc
     6961 actatgagat attcggctgg attcagaata tctcgcacaa tcttccccac ctgcgagcgg
     7021 ccggggatag tccgtacctc ctggaacgca ccgatacgct gacacgcatc tatgccagaa
     7081 tccggcaacg gcggtctgac acgctcaccg gacggcagta caaagccgcg gccctgtggc
     7141 aagtcgtcgg ccatagcggg ctgctcatca cggcgggact cctggtcgtc gatggacaac
     7201 tgacggtcgg gcaatttgcc gccgccgagg tgctggtcgg caacctcctg ctgaatatgg
     7261 acaccttggc tcggcgcatg gtcgcgatgt tttttacatt cgtgtcctgc cgggaaatgg
     7321 ccgcagtgtt ctccttgccg acagaagaag acggcgcgaa ggaagacgtg ccggcgacac
     7381 agttcggaga atcgggaatt cacctgacct gccggaacct ttcctacagg gcgcaggatg
     7441 gccttccgat ttttcaaaac gtgtctcttg aggtcgcgcc gggagagaac gtggccatcc
     7501 tgtgcggcac caacagggcc aaaaccgccc tcgcgaaagt cctggccggt ctacacccac
     7561 cttcgaccgg atttgtccgg tacaacgaca tgaatctcgc tgaagtgagg cgcgactcca
     7621 tcagccgggt acgcggactc atcctggatt ctcatccgac gctgctggac gggacgctgg
     7681 aagacaacat cacgctgggc cgacctgcca tcgaatacca ggatctccaa tgggccctgc
     7741 gttttgtgga gctggaccac gacatcgatg cgatgccaca agggctgcac acccccgtct
     7801 ccagtggaca gacacacctc tcgatgagtc agattctccg cctcctggta gcccgcgcga
     7861 ttgtgacccg cccgcaaatc ttaattttcg atggcacgtt gcacaatatg ctgcccgcca
     7921 caagagacct gctactccgc cggctctgcg cgaaggacga accctggtcg gtgatcttcg
     7981 tgtcgaacga tcctaccttc tcgactcatg tcgatcggca ggtgtccatc gagtagcggc
     8041 tcccgattcg catggaccgg caacggcact gaacgagaca gaatcgtttg accacccctc
     8101 catgcgtttt gtagaatgtg ccccgtgcac actgatcaac ggctgcgtaa aggaactgac
     8161 cccgctccat gactgtggct acccgcccat gggcttccgt tgtcatcccg atcaaggatg
     8221 aacgcgacaa tctggtccct ctgacagagc aattggtgaa ggtcctggac ggccgagaag
     8281 agtcgcgatc ggccccgttc gagttgattt tcatcgacga cggcagttcg gacggcagtt
     8341 cagacgtgtt ggatagcttg gcggcccggt accaaaccgt gaaggtcttt catttcgatc
     8401 gcaactacgg gcaatcggcg gctttcgatg caggattcaa gcggtcgaca ggagagctgg
     8461 tgatgaccat cgatggcgat ctgcagaacg atccggcgga tatcgccacg ctgctgccgc
     8521 acatcaacac cttcgatctc gtgtgtgggt ggcgaaaaga ccgccacgac aatctgacac
     8581 gaaagatttc gtcccgtatt gccaacagcg tccgcagcgc cgtcaccggc gatcgcgtcc
     8641 acgacaccgg ctgctcactg aagctgtttc ggcgggctgt ggtggaaaaa atgcagctct
     8701 tcgagggcat gcatcgtttt ttccccgcgc tggccttgat gcacgggttc acggtcaccg
     8761 aagtaccggt gcgccactac cctcgcacac gcggaacctc gaaatatggt gtaggcaacc
     8821 gtctgttcaa agggttgtac gatttggtgg ctgtgcgatg gatgcagcac cgctgcctgc
     8881 ggtatcacta ccggggcgca acaacatctc cttccggatc gacctcggcc tccgtaccac
     8941 tatgacactc gacaccattt ggctgattat cggattcctc ggacagggaa tcttcttcat
     9001 gcgctgggtg gtccagtgga tcgcctccga acgccatgct gaaagccgcg tgccgacggc
     9061 cttctggtat atgagcatga tcggcggctt gatcaccctc gcgtatgcca tctaccggca
     9121 ggatccggtc ttcattgccg gacagagcat cggcagtatc gtctaccttc gcaacctcat
     9181 gctcattcat cgcccgaact cggccgactc cagcacggct tccccggcga tcaaatccta
     9241 atggaacctc agatgaacgg agaagtcccg tccttgcaga cgccggcgcc gatcgaccgg
     9301 tcgattcagc cggtgacact cgttgtgttg ttggcgttgg ccgccgtgct gttctttgtc
     9361 ggactcggct ctcttggctt gaccgatcgg gatgaagggc gaaacgctga agccggtcgg
     9421 gaaatgttcg aaacgggcaa ttacatcagc cccacattca attacgagcc gcgctttgcc
     9481 aaaccggtct tcgtctattg gctgatgaca gcctcgtacc atctgttcgg cgtgagtgaa
     9541 ttcgcggcgc gatttccctc tgccctgttc ggtcttggcc tcattctgct ccagtatctg
     9601 tttctgacac gctgccgcgg accggtcatc ggcctgttcg gcgcggccat gctgttgttg
     9661 aatctggaga tcatcggcct cagccgcatg gcgctcaccg acagcgtgtt gatcttcttt
     9721 accacgctgt cgctctacgg attctggctc ggcctctacg gagagggacg tgaacgccac
     9781 tgcatctggt tgttctacat cggcatggcg ctagccacac tgacgaaggg gccgatcggt
     9841 tttctgattc cgctattggc ggttgggctc tatctctggc tgactcgctc ctggccgctg
     9901 ttccgtcgcg aggggcgtct cctgcccggc ctgttcctct ttgttgcctt ggctctcccg
     9961 tggtatctca tgatgttgaa cctgcatggg caacggtaca cgacctccgc gcaaggggat
    10021 accatcggcc gtttctttgg caccatggaa ggacacggtg gcaccctcct gttctatctg
    10081 cctgtgttca tcctgggctt ttttccctgg agcggcctcc tgccgtttgc ctggtatcag
    10141 gcctatcgaa gttggcgtga ggcgaaacag gctgggacct tcacaccggc gacaggtacg
    10201 gatacccctg cctcctcaca ggccctcgag tggtttgccg ctgcctgggt gctgggtggg
    10261 ctcgtgtttt tcagcctgtc ttcgacccgc ctcccgcact acatcggccc cttgtttccc
    10321 gcagccgcga tcttgacggc atccttctgg aatcgctgtg tgaccgacca tgcggcgccc
    10381 cacctgcgtg ctgcgatcca catgatgacc gccgtgggct acatcctggc gctggccttt
    10441 gcgtccctcc cgcccctgta tgccaaattc gccgtgaagt tagtggatga atttccgctt
    10501 gccggacagg tgacactcgg accggggccc tataccgtcg cctcgatttt tttggtggga
    10561 atgggtttgg tcgcctattt cgggctcagc gagacgcgga agccggccgc cttctgggcg
    10621 gcaggaggat ccttagcctt ggtggtccta gccgtcaccc aactgacgtt tccattggtc
    10681 aatcacttcg tgatcgaacc gccgcagcgg ctggccgacg tggcaggact gaatctcggc
    10741 ccggacgacc ggctgattct ctatggacag ccacgcccct cgctggtctt ctatgccaaa
    10801 cgaaaagcga tcgtcgtccc caagggtgag gaagctaaca ttaaacccta tttgacccag
    10861 ccgggtcgga cgctgattct cttgccggca gccatgcgca accggttgcc gttcgaaacg
    10921 atggaatatc ccgtgatgct cgaacggtac gggtacattc tgctcgccaa tcaatccatg
    10981 attcatgtcc ccgaggaggc agagaaacct ccgattcgga ttcccggaca ctgagcgctt
    11041 ttcactctaa tggcacaagg cctttcagaa attggatggc catcaccccc gcaagacctg
    11101 ccgccgtgta cagcccatcc ctgcgccagg acggctgggc aggtgcttcg agaacctgtg
    11161 ccgcagcatt ccatcgtgcg atacagagaa gtccggtgag cacgatcacg atcggtgctc
    11221 cggtcgccag gcctacccct gacagcaaga agcctttcga ggttaccacg gctgcaatac
    11281 ccccggcgga agtcgatcgc caccgaggca acatctgcac tagccaacct gcgagcacca
    11341 gcatcactcc cgacgccacc agtacggtat cggtcaccgg atcgagaatg cgatgcgtca
    11401 ccacccagca ccagcctgtc agcaacaaca ccatgggcgc gatgcgcacg agcgcatgcg
    11461 cgcacgagct gccccaccac cgcaatggtc cgttgaagat tgagccgacc ataaagccca
    11521 acaccatccc gccgatcaca tccccgggga aatgagagcc tctccagatc cggctggctc
    11581 cgacccacgc agccagggca aacggcaccc atgaaagcct gggcagcgct ctcgccaaga
    11641 ccgtcgccac ggcgacggtc gccgaggtat ggcccgaggg aaatgaatct aaccccgatt
    11701 gtaacgaggg ccaccagtgc catccgcctg aatgggtgag gcgtggcctg ggacggccga
    11761 tgatatgctt gaggctgttc accaccaacg ccaccacccc atgggcaagc aggctatcca
    11821 ccgccgtccg aatccacgcc tgtcgcttga gcagataccc ggctcccagg agaagcacgc
    11881 tgatggtaat caacgtcccc ccgttgccca gtttttcgcc aagatcaccc aacgattgga
    11941 gcgacgacaa attgtgtgag cggagtaacc gcaggatggg aatatcgatg tgaaagagcg
    12001 cggcgaacga cccgagcacc accagcgcgg aaaggatcac cgcaaggacc ggcggcctgt
    12061 ccggcggtgg cgcaggctgc gtcgataccg gcgcagcctg cccgtcacct gattcgctca
    12121 tggaacccag tcggcaagaa acacgttgaa ttctccaggc tccttcgcgt tgcggtcgga
    12181 cacccagacg agatgctttc cgtccggaga gaacatggga aagctgttga acttcccgcc
    12241 aaccgttact cgctccaggc cggagccgtc ttcattggtg agatacaaat gaaactcagg
    12301 ccggccccct tcattgcgcg tctccacgtt cgacgaaaaa atgacccggt gcccatccgg
    12361 atggaaatag ggagagaaat tggacgcgcc gttgctcgtc acctgctgct tgttcgaccc
    12421 gtcggcattc atgatgaaga tttccaggcg gcccggttcc acaaggtttt gcgcgaggag
    12481 tgatttgtac tgatccagtt cctcctgatt actcggatgc tgggcgcgat acacaatccg
    12541 cttactatcg ggcgagaaga atgccccgcc gtcgtagccg atttcctgtg tcagccgacg
    12601 gggatgcgtt ccgtccagat ccatggcgta gatatccaaa tccccgtcac gcatcgaggt
    12661 ccagataatg gtcttgccgt tgggagccac tgtcgcctca gcgtcatatc ccggggtatt
    12721 cgtcaatcgc tgcgggtcca agccgtcgat ccgaacggag aataaatcat agtcgtccag
    12781 cgcccaccgg taggcgccgc cctctcgctt aggcttcgga ggacaattgg ggcctcgtag
    12841 atgagtcgac gagtacagga cgcgccgatc accgggaaag aaatagccgc aggtcgtggt
    12901 gccggacccg gtgctgacca tccggatctt ctcgctcccg agatccatca catacatttg
    12961 gtaacagccc agggggatgt cggccggatg catggcagca gcaaacgtgt ccttcatcca
    13021 attgttcgtc gactggaaga tgagtttgtc gccgctgaac gaaaaatacg cctcggcatt
    13081 ttgccgccca aacgtgagtt gcttaatatt ggtgagatgc cgctcggtag aagccgtcgg
    13141 tgtcgcagtc tctgccgcct ttgaggcggc ggccgcgggg ttcgtcatgg aaagagaaag
    13201 catcaacccc agcatgcccg ccatcacctg tttagcgtgt ttcaattcgc acctctacag
    13261 tcatcctatc ctcccaatct ccccgtgcta ccacggaccc ttcgtgaaac accacataac
    13321 tctgccatcc gtaaaaaaac aagagccgag ccacacgagt cagtgccggc ggcgtcaccc
    13381 cgtagaggat ggtcacgacg ctgccaggct gatcgtctcg ccggcaggat accaacaggg
    13441 ccatcgtggg gccttcgtaa gactttcctt ccaccgaaaa tcctttttcg gtgaaatgaa
    13501 catggtcgcc gcaatatcgc agcatgtccg aggcggccgc attttccccc ggcccgccca
    13561 gtaccagcac cgacccctcc ggcgtaggct tcgtcttgtc ttccggttgt ttcacggtgg
    13621 tgcgagacgg ctcgggcttc gaggcttcct gtgcggcgat acgttgaacg atgtcggtaa
    13681 agggacccgg ttgttgtaag gaggctgggg ccaccaccac tgtgcgatgg gcgtctgtga
    13741 catacacatt caacatgggc gccaacgcat tccgcgccac gcggcgaaac acatggagat
    13801 ctggatccag ccgaaccgct aacgggcgtt cgggcagcgg tatgatcgca ttctgttggg
    13861 cgtcagtgag atgaatacgc gttcggtggg tacgtcctcc atccagtacg aattccagat
    13921 cgatggctag gcgatacggg gtaccatgtt gggcgatgtg cactatcgcc tccaccctgt
    13981 tcatgtcccc ggcagttccg gtggactgct ggacccggga cccagtcacc tcaatgtccg
    14041 gcacgccagc gcgctcgacc cactgagcga aaaaccatcg aaggtcgcgc cccgccgcct
    14101 cctggaacac tcgctccaga tcacgccact cggccgccat tccgagatat cgttccggta
    14161 tctgtttcaa agccgtccaa aatactgcct caccgatttc ctgcctcaac gcatgaaaca
    14221 ccatcgcggc ttgttgatag ccgatggcat tgtctttttc atcgtgcttc tgggcaaact
    14281 gtatgacggg atatgcctct ccggggcgca catagacggc atatcccatg agcatcaacc
    14341 gtcgttgttc gcgagcttgt gcctcgtcgc ccatgagctc gtgataatag taattcgaga
    14401 gataggtcgt gagcccttcc acccagttgc cgcgatcgat ccgattgaac acgccgttcc
    14461 cgatccacga atgcacaatc tcatggccaa gcgcgtaggg ctgggtgtag tgccgtttga
    14521 tcacgccgct gcccagcaga gtaaaggagg gcatgcccag tccgctcgaa aagaaatttt
    14581 cgacgacggc aaattgagaa aacggatagg gaccgagcag agggatgtac gcatcgagat
    14641 accgcgccga cgcgtccaaa tactcatcgg ctaaggaggc ttcatccgga aagagatagg
    14701 tggccagccg aatagattgc cccgactttg ccgtccaggt gcgggatttc accacgaatc
    14761 ggttggcgac caccgtcaag gcttcgctct tcgtgggaac tatccagtgg ccctggtcgg
    14821 actgaggagc tccttgcgtg acagccgtcc atccgtcagg cagggcaatg gtgacatcat
    14881 aggtggcgag cgaaccctcc acatcgggat accattggct ttcgctgctg agatagacgc
    14941 cttcgggtcc gatgtgcccg gacgtttcac tcggcgtgac aaaacgaagg tgccgcgggt
    15001 cacgtggcgg atcgtcgacc acacctcgat aggaaaactt cagggctacc gctccgtagc
    15061 cccgatcagt ggctggctgc ttcacaatta cgcgctgcgg ctcggatggg cctgctgaac
    15121 catccctctc gaaagcaagc tcaggagccg tttcgcaggc ggcctcacag tgcgtgacat
    15181 cctgaatgcg ctcgactcga agcgtagggg caagggagaa cgacagcgcc tgtccggaag
    15241 ctcttcccga gaggatcagg cgatccgtcg caatgagctg atgggagtcg ggcagcagct
    15301 ggagcgtcag ctgatgatgt gtgatagtcg gtagggagga aacaggctca gcaactgcaa
    15361 gcgcaggcac gagacaaccg gcaagcagac tgaaacggat gaggcgatgc aaacaatcca
    15421 cccttcaccg gcaccccgag gttgacggtg aagattagca ttcccgacag accatcacaa
    15481 gctggattgc catcagggcg cgctccaaat caagtggttt atccagcact tcttgcggcc
    15541 ccaacgccca ggcgtcctcc agcaatggat cattttgatg tccgctcaaa atgatggtcc
    15601 gcccggcgta ctcgcgtgta gccagcactt tcagcaactc cacgccgttc atctccggca
    15661 tgacaagatc aaggatcaac aaatccggcg cggactcttc aatcatccgc aaggcttcgc
    15721 ggccatcctt agccccacgc acacgatagc cgcgcagact gaggaagcgc atcagcagat
    15781 cacgtgccat cacatcgtca tcgaccacga gaatctgttc ctcggaaggc tgatcgatct
    15841 cactcgcggt tacatcctgt gtggccgacg cttcacgtct cgcctgttgg gcgatccgat
    15901 gcaccgctcc caccagcaca tccagcgaca atccctttcg aaagaaatct gtgacgcgta
    15961 attttcgtgc gagattttcc agctcctcgg tagcaccccc tcccagaata atcactccgg
    16021 cacgcgggtc atacgtccga atctctttga gcaccgccag cccatccatt tccggcatgc
    16081 gcaggtctaa caacgtcacg accggccgaa gctgccgaaa gatcgctacg ccctcacgcc
    16141 cgcttgtcgc cgtgctgacc cgatatccat ggcgcgacaa cgctacctgg aggagatcac
    16201 agtgcaaccg gtcatcgtca atgactaata atgtggtcat agtcgattct cacccatatg
    16261 cgacaggcct taccctcagc cttttccggc cgaagtctca cgaaacagcc tagcatgagc
    16321 ttcgtaggat gagaagaaat tgacggaaac tgtaggaagg gcagcagaac ggggccggtt
    16381 cagctattgg tcaaaacaat gcgtcgacaa atgccttggc gttgaaatcc tgaaggtcct
    16441 cgacggattc ccccacgccg atcaatcgga ccgggatttt gagggtatcc gcaattgcca
    16501 ccacgatccc cccgcgggct gtgccatcga gcttggtcaa cgcaatgccg gtcaccccga
    16561 ccgcttcgtg aaactgtcgg gcttgcgcaa tggcattttg tcccaccgtc gcatccaaga
    16621 ccagcaacac ctcatggggt gcacccggac actcctgcgc cacaatccgt ttgattttgc
    16681 gcagttcgtc catcaagttg gttttggtgt ggagtcggcc tgccgtgtcg atgaggacca
    16741 catcggatcc tcgtgctttg gcggccgtaa tcccgtcgta ggccacggct gcggggtcgg
    16801 cacccggtcg atgccggatg atgtcgacct gggtacggtc cgcccacact tgcaattgat
    16861 cgatcgccgc cgcccggaat gtatcaccag cgaccagcag tgcggtgttt ccttgttgcc
    16921 gaaaccgttg ggtcaatttg gcgacggtgg tggtcttgcc gacaccgttc actcccacgg
    16981 cgagaatgac gaagggacgc ggcccttgcg cgatgagttg gtcgatcgac gccccttgcg
    17041 tcggcaagag gatgtccaag acggattggc gcagcagctc ccgcaccttc tcggcagccg
    17101 agaaattccc tccgcgcatc tgagcacgca ggttctccat gacacgttct acgaccggca
    17161 tccccaaatc cgcgctgatg agggcgacct ccagctcctc taacagggcc ggatcagccg
    17221 cgcgtcccag aaggcgatcc agttgcccgg tcaccgcatt acgtgttttt gagaggccct
    17281 cgctcagttt ctgaaaccaa cccaccgctc acctcannnn nnnnnnnnnn nnnnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnntacg ctttgatgat ctcttggacg agccgatgcc gcacgacatc
    17461 ccgttcatcg aaatacacaa actcaatccc cgcaatatcc tgaaggatgt tccgcacttc
    17521 aatcagcccc gagacgcggt cggatggcaa atcgatctgt gtgatatcgc ctgttacgac
    17581 ggctttggaa tggaagccta accgggtgag gaacattttc atttgctcag ccgtcgcgtt
    17641 ctgggcttcg tcgagaatca cgaacgagtc gttgagggtg cggcctcgca tgaacgcgag
    17701 aggagcgatt tcaatatctc cccgctcaat gagacgattg gctcgttcca tgtccatcat
    17761 atcgaacagc gcatcgtaca gaggacgaag atacggattc accttcgcgt acatgtcccc
    17821 cggcaaataa ccgagttttt cgcccgcttc gactgccggc cgcgcgagga taatgcggct
    17881 gacatccttc ttcatcagcg cgctcaccgc catagccata gccaaatagg tcttacctgt
    17941 gccggcagga ccaatcccga tgacgatgtc gtgcttttcg atcgcgtcga ggtagtattt
    18001 ctgtgtcggg gtcttaggaa cgatgaagcg cttccgggtg acaatggggg acgcgctgaa
    18061 gagcagttcc ttgagggagg cgtcgtgatt gtggcggagg gcggtcaatg cgtgagtgac
    18121 gtcatccgac ctgagttcgt agccttcgtt ggtgagcgat gcgagttcat tgagtactcg
    18181 ctcggcctgc ttcacggcat cggcagtacc ctctaccgtg acttcttcgc cccgtgcgga
    18241 acaccgcacc ccgaactctt cctcaatcaa tttgaggtgg cgatcgtggt gtccaaatag
    18301 gaccgcagtg ttcgtgcctt cacgtagttt gatcttgcgc acgcctgtgt cgaagactcc
    18361 tttcgccaag ctgatatcta gattctaaca aaccacgaaa gggaggaaca aggtcggaac
    18421 aggggggtgg ggaaaaatta gcggaactga ggtctcgaga agccggctac ccttgaggcg
    18481 gcaacgaggc ctgcgattgc gccgtggagg gaatcgagag ttcaaactcc tgccaggcaa
    18541 ttccgccctg cccgtcttcc gccatgatct tcactcgatg agttcccgcc actcccggaa
    18601 tgactttcca gctgacctgg cctgttgccc tgtcaatggt cattcccggg gggcctgtct
    18661 ccaagacata gctcaccgta tctccatcca catccttggc ctggaccaca tactcgtatt
    18721 gttctcgatt cgtgagcgcc gccgggttgg aaacaatcaa cggcggtgaa ttccccaacg
    18781 cgctcggggc cgaccggacc ggcgcagctg ctgcatcctg atcccgcgca atcacttcaa
    18841 cgaccaccac atcttttcgc ccaaatcctg tggtgtcgag gatgctttcc tcaccttctt
    18901 tgacctgttt gtcgttgcgc caccaccggt agttgtagtg gatttcatct cgatcaggat
    18961 cgacggcatc gaccttggca agaacccggc tgttatgttc agcatctgcc tcaacggtga
    19021 catgtgacac gagaggggga gtgttcacca cagaaaccgg ctccgtgcga tagggagcgc
    19081 tttccagctc accatcgaag gctacaacct ccagcgaaac ctggtctcct cgcttcacat
    19141 ggaccggatc cagttcaagg ccggttgccc ccaaaacagg agcactgttg acgatccact
    19201 gaaatcgctt ggtaggctcg gtaccatcag ggtcatctgc catcacatgt gccgtgatag
    19261 gtcctgcgag tatgagtggg tttggcacaa tcgtcacaag gcgaacaacg ggagcgtggt
    19321 tattctttgt cgcaccactg accactgatc cgtcttgcgg agaggacgag caccccccta
    19381 caacggcgac gaccgaagca gccaacagcc atcgagcgga tgaaatgacg gtgtggggac
    19441 agagtctttc cccacacctt tcaaccaggc tccagacacc agtcttcatt gattggtcca
    19501 tgagaaaaat ctactccgcg ggtcaatcgc gacaggaacg ctgatgttcg atccaaacat
    19561 gcctccacct gtcgtgcagg tcgcacagtc tccgaaattt ccctggctca tgttgatcag
    19621 caacccgaac gggcttccgc cgtctctgcc ggagcccata tgcaccacga caccgaatgc
    19681 taacccttcg cctagtgatc caaatttgtt gaccgtctga ttcgcgcctg ctcccgtcgt
    19741 acccaaaatc ggctcctgat aggcgcttcc tgttttgtag aacagggccc agagccggct
    19801 ggtgccagac gacacgcaga tatcattggt cggaataaag gtggggaaaa atacgacccc
    19861 tccaaacacc gtcgggctac tgacggcacg ttctccgata ccatacggaa ccggcttcgt
    19921 gggtggattg gcaggctcga cgagcttggt cacccatcca tccttgcttt ggaccaaccc
    19981 aatcagtcct gtgaacgtgg tcgcgccggt cacaccggtc acctgatttg taccggtcgc
    20041 cagtccgcaa tcgccaacgc ccaccacaca cacagtggca ttggacacat ccacgagatc
    20101 gctgtccatg caggcggtga taccgcttga ttgattgcac ccagcattga gtactgaatc
    20161 cttcatgcct acgaagtact gggtggagat gtcggtctta tcagacttgc tgtaataacg
    20221 gccgctcccc acaaacaccc agacattagc cgcgtcatct gtcgtgacgg aaggggcgct
    20281 tgcaaccggg cccatctccg ctccggacgc aaaggtatcc aacatttccg tcgggacgcg
    20341 cgcgctaccg gaggaaatcc cccattgcga agggctcgtg accgtaccga attttgaggt
    20401 tgccccgcct gcccccatgg tcagccgata gatctttccc ctccaaggga gagccccatc
    20461 attgatgacg cggccgaagt agacgacatc gtgacgatag tcgaggtcac gatcgaacgc
    20521 cgtcatatct ccgatgtacg aatcccatga cccgatggaa tatgaggtca ccaaactgtt
    20581 actgcttccc ggtcctgtcg cgagatcgat ggcaaacact ttcgcgccct gcgccacgct
    20641 ggcgtcgtac ccggtcgggc cggacccgac gaccatgtac cacttcgcgt tcgtattgct
    20701 ggctttggca tcggcggccg gattcacacg cacgaccgcc gggacgctgg tcgacaatcc
    20761 caaatccgag ctgctgaatg accacagcaa tctcgggcta tcgggattgg tgacatccat
    20821 cgcgaaataa gcgctataga aattgtagtt ggttccgcca atgttcaccg tcatcggcgg
    20881 tgcaccagtt cccgccacac aggcaccgca actgcctccc attctgaagc cgccgatcag
    20941 aatagtgccc catccattgg gatgatcggt atccgcggtg aagatccgca catcggcgac
    21001 cttgagaggc aagtcaacat agtatgcatg ttgataatcg gcacggctca gaaactcgag
    21061 ctgagggagg agttcatagg ggacaaatcc ccataactca tccccaagcg gagctccgct
    21121 ggaattgtcg gcgggagccg tcgtaaacca accatgttcg gttgtgctgg cgggcgcaga
    21181 cgtgctcgta tcgtcaccgg gatggtagta accggcgttg aacgcatgca gcatgccgtc
    21241 atttccgccc acgtaggcaa cctgtctgcg gttgtaccac ttctgcagga acgcggtgta
    21301 actctggtct ccgtagatcg catcgtgacg gtctcgagga cccgccacga cagtcggcgt
    21361 cgagtggatg acgtctccca gcttccacac ttttaacgtt ccgcttcccg aaggaacctg
    21421 gagacggcga tctctggtcg tcgcttgatc agtgcatgtt gtggcatcac atccacggat
    21481 aaagttaata attttggttc cctctgcact ggacgtggca cgcaggtatg gacgaagcgt
    21541 tgtttcgttc gcggtggaga acgcgatctg ctcaccggaa tccaccactc cgtcatggtc
    21601 ggaatcgacc caagtgagaa tggtacgggt ggatgcatct tttaacgcca attgcttccc
    21661 ggcttcccag atcggcaata tgtcgctcaa tagtttgccg cagggattgc aatccgccag
    21721 cgtgacgacg ccgtcgctgt tctgatcgtt cggcaatcca tcgccatccg aatcgacata
    21781 tttatcgacc ttcaccgagt tagagagtga gtcgaagcgg gtcttgatga tcttgtcgac
    21841 cttgtagtca aggcgaccgt cctggttcgt gtcctctcgc gtgttgccga acgtatcgat
    21901 aaacaatgcc tgggtatatc ccgtccattt cacgtcactc aacgtcgagg gctcgatcgt
    21961 gctgggatag aaataggatt gatacagcgc gccttctcca gttgaagcag tggccaatac
    22021 cgacaccgaa gaacccgacg cgctccgccg gagaatactt gcaatggtgg ccaacaactt
    22081 gtcttggaga tcgtccacgt tcgaggattc gaaatacgca tccggaatgc cgtcgggtgt
    22141 tgacgctccg gtcaaattgt tctctttgtc ccattcggaa gtctggggga tcccatctcc
    22201 gttcgcatct tcaaatcccc ccaagcgggc ggtttgggcg agaattcctc gaccattgat
    22261 attgccaaat gcaaaaaatg aatacacggt gagattctgc gtgccgggta tgcaatgccc
    22321 agtgacagat aaccctgcga tcgtgccgtc gctcgttcca ctgcaagggc gcaaatcact
    22381 ggtgtgcgcc caataggcca catcgtctaa gtaatggctt ccgctgtcgg cgtaatcagt
    22441 cttgtgttca gccaacagat cactgaaggg ggtcgccgca ttagtattac aagtgccgtt
    22501 gataggccga ggaggagcag cgctatcgct gccgttacaa tgctgcccat gatgggcatg
    22561 ggcatagtcc tgaatcgccg tcggtacatt cacgtcttgc gtcggctcac cgtctgtaaa
    22621 aataatgacg aacgtccggc agcaattgac gacctgagaa gtcgacgccc aaggcggcgt
    22681 gtggttttgg ccaaaaaagt aggggtcgcg gccacaggcg ttcgtgatgt atccagccgg
    22741 gcaggtttcg gtaccaacca atgccgtgat ttcgtccgtg cctatggaac cagcaccggt
    22801 ggtggcaaaa ttgactccgt ttgaaatgcc tcctgaaaac gcgatcggat acacgtagcc
    22861 tgtgacaaac gttgattgaa tctgcgcaac ataccgtgcc gtctcataga gtgattcgct
    22921 cagtggcgtc caggtggagg gatacgactc ctgcaccgcg tccaccatag ccgccatgtt
    22981 cgtattgaac gtctctactc ctgtaccatt ccagtcaatg gattgacgag aacccgcgcc
    23041 gactaacatg cgcgcacctt cactactgga cttgaattcg acgaggccga agcgagcctt
    23101 gctcccgatc tgctgaatga cccccgtggg ttcagtcgaa tacccaacct tcaattcata
    23161 cttacgaacg ctgtatgagt ctccgcaatt actgttgaaa ctggtatcat tgtccacaca
    23221 aaaatacggc acagccggag ttccgctcgc ggagatacca atcgagatcg tccctggatt
    23281 gctaccccga tcggctaacg gaatgcgccc cacatacgtg ttggctccag tgccaccgct
    23341 gtaattcacg tcggctcgtt catcgttgac cccggtggcc tgggcacgca cagttttcaa
    23401 cgcaggcgtt ccactggtcg ggcaggtgcc atccgtcgct ctatttacgg cacagtctcc
    23461 tccggacatc gcccgtttca ccgcatcgaa ccggcgcagt gtcgcccaat tcaaaaaatt
    23521 gccatcccat tccgtcgtcg aacaatccgt gtacacatat gcttttgcgg tagcgctttc
    23581 aaatctcttg tcagtcgtgt tgtaggtata gcagcggaga gaatcaaagt agcccgaata
    23641 ccgcgtggta ttagtccacg ttgtcgtcgt cgacgtactg ccatccgcca atgtgccaca
    23701 agaggtggat tcgcacgctc gattgctcat gctgccggaa ttgtccatca agatgatgat
    23761 attcggggtc gtcgcgctcg acacaaaggg cggtactgcg gtgtaatccg cattggagat
    23821 gacggctgcc gcatcgccgg acccacacag accggcactc acgctcccgg cgaagatcat
    23881 cagcgaggca agagttcgtc gattcatgcg ttctccctcc ttatctcaag caggacaaca
    23941 gccgtggtgc gtcggctacg agttgagaca agacctttca tgcgctattg cggcaacgtg
    24001 accatcatct tgtcgatgtg gccttccttc agatggaatt tgacgagcga cgtcggaatg
    24061 atccgctcca cagccaacgg ctctccctcg tgtgtcacga ccactacggc agatttcaat
    24121 acatacgtcc gcccatcaat gcggatcgtc gatccctgca cctcatcgat gacaccggac
    24181 tgaaagccga ccgtcgaaaa caggattggt tcttgagctt gttccggttc catccccgcg
    24241 acggatggtc ctgggttggc cgcctgcacc aacatagaaa ggctcaggat tagaagaagg
    24301 atgaggtgat tcatcgtcat accctctctc aattgacctt tttcacgcat gtatcgccgt
    24361 ccaaacatcc atagaccgat gtcactgtgc tgcttgaccc agtggtggca ttcgtcgccg
    24421 tacaggtgat tcggtagaca aattctgttg aggtcactcc agtcccctga ccggccttgg
    24481 aatgggtata gagccggtcg atgtcaccat tcacggtaaa tcccgccaca ttcgtcatga
    24541 caaaattcgg gtccattgcc gcggaatctg catagttttc agccaacgtt ccggtgggcg
    24601 ccggtgatgg aagcggcgag gaaccaatga tctcctgcca gagaatcgtc gcattggctg
    24661 ccggaacagg ccctgtcggt gcgacaaacg aggtcggcag cacgccaggg ggtgataagg
    24721 tctgctgaat gaggttggca gcaaggcctt cgcacgaatc tgccgccgcg acgacggctt
    24781 cggttgtgcg gaaaaacccc gccattcggt tctccatgct ggtcaccgtg atggaagcga
    24841 taccgaggat cgacagaatc agcaacatca gcatgacggt gaggagcgcg atgccttgct
    24901 gtccagtcac tcgactctcg ccttgcatca gttgtctagc ttttgacgcg tgtcgcacat
    24961 tcatcgcggc gtctctcatt acaggtttcg tggttgcact gtgcgaatca ccacccgtct
    25021 tcgttgctgc tgataggtct gcgcgttata accggtgtcc gtggatgggt tgtgatcacc
    25081 gacgatgacc ggcccagtcg tattgacggc tttggttcct ttttcatcca acccgtcatc
    25141 cgcctttgtg ggtctcacca caaggctcac ttgcgccatt ctgatcttgt ccggcgtcct
    25201 cggtgtaatc gcccatgaac cgttcgacac aaaatctccc tgcgtgaagg ttggaaaccc
    25261 actggaggac gagccatctt ggtcgtcgat gataccgtcc ggatacaggg gatttggcgc
    25321 agcctggttg catccgtcac annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25441 ntgactattc acaacattgg ctcggttcgc cgtcaccacc gtcgtcatgg tcgccgcgac
    25501 gatcaccacg gtgatcaaca ccgcggccat caattccacg agcgtgaagc cctgttggtt
    25561 gcctgtcact cgacgcatca tgaggctctc catcgcgtct attccggtgc aatcaccgtc
    25621 gtaaacaccg cgctcttgtt gcgcgaggtg ctcacatcgg tcttttgggt acgccagttc
    25681 accgtgatgg tgaccaaaaa gcgattcatc gtcacaggat ttgccgtggg atcgggatcg
    25741 agacggctgg ctgtcacggt accaacagcc gactggagac cggagttcgt gaccaacgct
    25801 gaccattggg tacagtcgcc ccacgccatg cgttgcgtgc tcacactctg tgggcaggcc
    25861 acgttcgtcg cagtggcgtt ataatccaac acccgctgac ggttactctg aatacgctca
    25921 gccatatcca tggcaatatt cgtgacacgc gacatgtcgt tggcatcaac atttttcccc
    25981 agagacattc ccgacaatcc cgcaagtcct agtagaccga cagaaagcac gcccgctgcg
    26041 atcatcgcct ccaacaaggt aaatcctagc tgacgcggcg tgatcaattg tgaggacgct
    26101 atgtattcag tcgtgtagct catgcagcct ctatgggcac gtggcgtggg cgcaccagtt
    26161 cactttgcct gacggggtga cgaccagcga gtagacggtc ccttgttgag aaaccagggt
    26221 cagcggcacg tttgcggttg atccgcttaa tccttgtcgg gtgaactgca ctgtggggcc
    26281 cccggtaaat ccaactatcg cttggggcag gacgatggga gcgagtgccc cgccgaaatc
    26341 gacggtcact ctcccggaag ccaacgccag ggtcaccgtg acggccagat tccgattcat
    26401 cgcggccata cgggccagcg tcagactgcc tgccaattcg gtcgtgccct gcttcagctg
    26461 atagcgggca ttccattgca gataattagg gacggcgacc atggccaann nnnnnnnnnn
    26521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26581 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt ctgcgagtgg acctgctttg aggcagattc
    26641 atcaacaact acggacaaca acgctactta atagccactg cgcgcacctg ctgatatgtc
    26701 gtgaggcctt ggaggacctt cccgatgcca tcctgaacga gtgtctgcat accttcactt
    26761 tttgcgagtg ccagcaattc tccggtgcga ccccgtcctt gaatgagcgc tttcatgccg
    26821 tcggaaacta ccattaactc atgaattggc actcgcccgc ggtacccgct gtgatggcac
    26881 acctcgcacc ctcgtccgcg aaacaacgtg aacgtgtcat catgcgcggc ttgccgggat
    26941 gcccagtccc tctctccgaa cgcgtgcacc aattcatcat attcgctgcg agtcggatgg
    27001 tagggctccc gacagtgcga acaaattcgc ttacacaacc gcatcgcaag gacacctagc
    27061 atggcatcag aaaagttgaa tggatcacag cccatatcga gcaaccgcac gactgtttct
    27121 acggcgctat tcgtatggat ggtgctgaac accagatgac ccgttaacga ggcttcaatg
    27181 gcaatatccg ccgtttcctt gtcgcgcatt tcaccgatca taatgacatc gggatcggct
    27241 cgcaaaaagg ccctcatggt ggtggcaaac gtcaggccga tcttcggatg cacctgcacc
    27301 tgtcgcaggc cgtcttgcgt aatctctatc gggtcttcgg ccgtccagat ctttcgttcg
    27361 tccgtattga tagatgccag aatggcgtgc aatgtcgtcg tttttccgga tccggtcggg
    27421 ccggcgcaca acacgatgcc atgtggtttt tgcgccaact gctcgactac gcggcgcgtc
    27481 tcaacgctga actcaaactc ttcgagtcgc ctggggccat tagcgctcag caggcgaagc
    27541 acgacatctt cattaaatcc tgcagtggga agggtcgcga cacgcaattc aatctcctgg
    27601 ccggtcgtca atttgaagcg gatttttccg tcctgaggct tccgccgttc ggcaatatcc
    27661 aggctggcca tgatcttcac gcgagataca atcgcccgac gatacgcggc aggaatcttc
    27721 atgtaggtaa agcaggtccc atcgacccgg aaacgcacgg ccgtttcttt gcggtcggca
    27781 tagggctcga tatgaatatc cgatgcacct cgccgatagg cctcggcgat gatctgattg
    27841 gcgagacgaa cgatggccga atcattttcg gtgatcgctg ctatcgtggc attttgctgt
    27901 tcttctaact gcgcttcgct caccaactcg ccaagaatat ccgtgatggg atccccgacc
    27961 tcacgcccgc acgcatcact caacaggcgc tcgatatcgg cccgcagacc aacgcggtat
    28021 aggacagact gccctggaaa ggcgcgccgc acatcgagaa gcttgtcagg gttgtgtgga
    28081 tcatcgatca aaacctccac cccctgtgct tgacgtttga ggggaaccca atgattcatt
    28141 ctcagatagt caatgctcag gtttttcaat aactccggct ccacgatagt ctgctcatca
    28201 taggcgagaa actggcattc atagaattcc gcaaggactg cgccaatcgc cggtttggga
    28261 attctgtacc ggtccatgag tagcgtggtc acatccagcc ttcttctcaa cgcttccgag
    28321 aaggccagcg ccagttcctc ctgtcggaga aatccctggt gaacgagtgg ctcatatagt
    28381 ccggatggac ggctacgagt tggccgtggt tttcctaatg tgagtccgtt tgacaaacac
    28441 gcaggggaac ttgcacgaat catggcccta tccttttcaa ctgatttaaa catggggtga
    28501 tgatctgatt gcgagagggc gctcatcctc tcacagttta gtctgccaac taccgggtgc
    28561 ggcaaatacc accggcaacc cctggacaaa cccttcacga agatcgtggt tgtaccaaac
    28621 ctccagcatt ccagacccat tggccgcttc cataatcgct ccttgcgccg ccaccccacc
    28681 gtagactttg agatgcccgg caaatcgcac atcacctgcg gcatacaaca cgccttgaag
    28741 atgaatgccc gagagctgca ctggaacccg tgccccaagc gactgctgcc cctcgggcgg
    28801 cgggcttagt gccggcaagg ccctccccga acggccagcc ttccacagca catgcgcatt
    28861 cacaataaag ataccctcgg catagtcctg atcgagcacg atcgtgccca agttatcgaa
    28921 gcgaggcggc atcccatcaa gggtgtctac aaacacgagt ccacgatgat cgcccggtcc
    28981 ctttgaagca aacacctcgg atgcaggacg ccccatgccg gcctgaatga caccgcccgc
    29041 atacaataat ccctctcgat cgatcccgta cacctggcca aactgcatcg ccattcgctt
    29101 caaggtcgca tactcccact gatccacctt aatgccgggg accggctctt gatgcgcatg
    29161 cacattcaat ggcacgccgg accaagcttc ggccggaagc tgggcaaaaa aagcatcgcc
    29221 ccccagccgg atcgtcaacc agcgatcttc ccgatgactc atttcgtcat agacttgtcc
    29281 actcacagga gccagtgcac tcttgactgg aacgtcttcc gggcggggga gataggctcg
    29341 tccgcgcacc accatttcac cccaatgcgt aaaaaccgaa ccggaacggg attgcaccgc
    29401 ctcgctgcca agggtgccac tttgcagggg cgcatgaaga gcaggaacgg cgtaggcccc
    29461 gaattccaag ctcaacgttt ttgtcacacg ggaggcaggg ccggccccag ccgtcacttc
    29521 gaccgtgcag agcaaacccg gtctcgtcgc tccatagact cgtaatttga gaattttcgc
    29581 gagacccttg agcgatttga accagcctgt ctgcggatca ttcaggagtc ggtcgtgctg
    29641 gatgttggct gcatcgaaca ctacgtcagg ggccgtgctc gttccgatga actgtccatg
    29701 cccctgcgcg tcaacatatg aaggatctcc ctgtgcattg accagtcgtt tgctaaacca
    29761 ggtcgtcccg atttcctgtg gacgaagtgt cggatcatga aaccacccca tcaccacatc
    29821 cacagccgcc tcagcagcgt gatgcgcgag ccgctcttcc tgcaatgcac tggcaccaac
    29881 gacttcctgc cccgccaact gcatggatgt catccccaag agggtgagga gaaacaccac
    29941 catcagcact gctaacaatg ccattccccg ttcatccccc tgccttctct ttgctgatcg
    30001 ttctccgctc ctattcatat ccccggtccc ctttagttac attagtcaca ttcgtatcgc
    30061 gatatctcgc gacaggttca ttccttgccg agccccctgt atcgttactc gaacgcgcac
    30121 cacatcccgc gcgtcattgg ctatacctcc gcttttgttg aaatactgca gctgcattcc
    30181 gctcaactcg ctcaacaacg tacttgccac gccatccact tctcgcatca gccgatgtgt
    30241 tccttcagca cgctttaggt agtaccgcac acggttcagc aaaaagatgg cggttttcgg
    30301 ctgatactgt tcgagagtcg gcgtcatgag cgtcagcgtt tgctttcggc catcggcagc
    30361 cagacgattc cagacacaat gcgccgcagt acacagaagc agctgcttgc ctttcggcca
    30421 atcggcgccg tcttctaccg gcaggtcctg ccggccgact tctactatct gcgtcaggat
    30481 ggttgacgat ccgttcaaat tggcaaaaaa ttcgacttcg tccacctcca tctttgaaaa
    30541 gacagtgtct ccgctcaata atccgccacc ggccaagcgc agttcgctac acaacacatc
    30601 cagccctagt cgcagctctt gattggccgt catggtagcg tgctgggaac tcaagcgaga
    30661 gccataggcg gcagaggttt gaattgccgc cgctatcgcc acgctgctga tcgccagcgc
    30721 gatgagaagc tcgataaggc tgacccccct ggtgttgaga caccatctcg ccctgatcgg
    30781 cttcgtctgt tgtgaatcag agagtgtggc gggtgtcatg agagcgccat cgcaccgacc
    30841 gcgcgcggat ttgcccggat cgtacgcacg cgcacttcac gcgactggcc gccgagcgtc
    30901 gtgaaactcg ctcgcgcctc aatcgtcgcc aggctggcag ttccaggctg ccctccacga
    30961 ttgagttcca ccgtccagac cagtctgatg ttggaccgcg tcacactccc ggtgaagata
    31021 ccgtcgccat tggtctcatc atccagcagg ccgtcgtcgc gcatgagaga ttccagcgtc
    31081 ccgttctgat cgaggtcatc gaccaacaat tgatcccaag gcagactacg cttcgcctcc
    31141 aggcacgatt ctgcgagagc caacgctgtc gtcgtcatcg caccgcgttg aaggccacgc
    31201 tgcgcccact cacacattgc gatcgtccca atgactccga tcgacaggac caccaaggcc
    31261 accatgcttt ccaccagcgt aaaccccgct gtgctcgtgg tcagaccttg ccatgttgcc
    31321 tgcttcccct ctggcttttt catgacagtg acacccgtcc ggtgaggctc accgtgacct
    31381 gatgcgtcac gtgtctggaa tcaatcaaga ccacggtcgt ggccgtggcg gaacggccgc
    31441 tggattggaa aagaatctcg ggcctggtac tcatcgagtc gacaactgtg cctgtgcgtg
    31501 cgaactcata gcgacggagc acagtcgtct cacagtccac acattcagtc cgcaactcgg
    31561 attgcgccgc attcaccacg actcgcacac gttcgtgccg cgccatggcc agctgccggg
    31621 ccatgcgtaa ttcagatgcg atctcagcca ccacggcccg ctgtcgctgt ttggcaacaa
    31681 gtgccgacca tcccggaaca ctcaccccta tcacaatcgc cacgatggcg agcactgtgc
    31741 acagttccac tagactgact ccgcgttcat tcatttccgc tcctttcttc gagcgcgtag
    31801 agcgtttcca atttccgtgc cgttggttgt tcacgcatcc ggcaggaagt gcgagcggac
    31861 cgggttttgt ggaaaacccg gctaagaggc cgagttggcg ggagtttgcg cagacagagt
    31921 ctgcactcag acggtgaatt ggccagtgcc aatggagttg gacaattgtg aactgttcct
    31981 aatcagatac agtgcgccaa ccattccgat acagacagcg gaataatcaa tgcctacaaa
    32041 ctggctctga cagccgattt gcagagattt actggggcag gctaatgaga gaccaatacc
    32101 aattgaggag aggctgatga aacagaagag cgagcaggga ccccgccgcg aggaacggtc
    32161 caaatggaat atattgatcc cgtttggtga gcccggcggc gatcagcgcg acaccgacga
    32221 gggagcctag aaacgatccg atcataatgg ccaacaatac cggctgccac ccaatgaacg
    32281 caccgaccat cgccatgagt ttaatgtctc cgccgcccat tccttctttt ccgaagacat
    32341 aggggctgac ccaagccagg aaccacagaa tacctccacc cgcgaggacg ccaagaagtg
    32401 agtcaatcag gccaatcggc agaatcaccg cggcacagat gaggccgata gcaattcccg
    32461 gcaaggtcac agcatcagga atcatcgtat gagaaagatc cgtccccgta atcacaatca
    32521 atgccgatag taaggcggaa tacacgaacg cctcggccgt gaagtcaaac atccaaaaga
    32581 tcagcacgta tccgattgcg ttgacagctt ccaccaacgg atattgggtg gaaatgggca
    32641 cctggcagga acggcaccgc ccccgcagaa gtagatagct gacaacagga atgttgtcgt
    32701 ataccgcgac aggttgccga caggcggggc aatgcgaggc gggccaggcc acagactctt
    32761 cgcgcggtaa tcggtagata cagacgttca ggaaactacc gatgaccgca ccaaacaaga
    32821 acaccatcac atatgggatc atcacgcctc ccgccacggc atgctccttt ccatccacgc
    32881 acgtgcatct aagtacacgt atacgcgcct aggtcaataa gagcatgcgg tcccccctct
    32941 gcacccgcct cttttcattt acaaacgcca ggagcactgc gtataatccg acaacgtttt
    33001 gggccgagta gtcgaagatt caatctgaga attgccatat tccaagtgga gcgagacgta
    33061 catgccgaca gctgccagga ctaccaaagg tcgaaccaaa gccgcatcat ccgagccgat
    33121 tcttccccct cgcaagcgac gtccggaagc cctcaccaca cgcgaacagg aaattcttga
    33181 attgatctgg accggcttca agaacaagga aatcgcccag cggctcaaaa tcagcgtgaa
    33241 gaccgtcgaa gcacaccgag ccaacatgat gaagaagatc cgggtgtcga acacggccca
    33301 gctgctcaag gccgccattc aaggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33421 nnnntgaaga agatccgggt gtcgaacacg gcccagctgc tcaaggccgc cattcaagga
    33481 aaaatgctca agctgagata gtccagcggt ctcagtgtcc gactcgatcc tctcctcgtt
    33541 cccgacgctg acgcacagcc tcatacagaa cgatggccgc tgacgccgag acattgagcg
    33601 agctgacctt ccccgacata ggaatgtgcg cagcatcatc gcaggcgccc cgaacccctg
    33661 gccgtactcc ctttccttct ccaccaaata ccagcgcgac aggcccccgg aagtccagtt
    33721 tcgtatacac cttcggcgct tcggcatcca gcgcatacac ccagatcccg gcctgctgca
    33781 ggcgctcaat ccaccgcgaa aggttgggaa ctctgccgac cggcatataa tccaccgctc
    33841 cggctgacgc cttggccacc acactcgtca gccccactgc acggcgttcc ggaataaaaa
    33901 ccccctgcac cccggacgct tctgccgttc gtacaatcgc cccgagattg tgaggatctt
    33961 cgacaccgtc gagcagtacc aacagtggag tctgaccggg ggcttgcgcg gaggcaagaa
    34021 tagcatccgg atcgctataa gacttggcgg cgaccaaccc aaccacgccc tggtgatggc
    34081 cggagggaac aagtcgatcg aaagcagcgc gtggctcgac atacacagga atgcgctgct
    34141 ctctggcaag ccgcacgatt tccccgaact gccgatccgt ccccaacacc agcagtcgct
    34201 gaatagggcg agcccctgca cggagtgcct cgcgcacggc atgcaggccg taaattacgt
    34261 ccggcgtgtt atcttttcca tcgactggtg ccatcaggcc ggtcctcgat gataatgccg
    34321 tgggatgcca actcggctct taattcgtca gccaacttgt agtttttgga ttgtttggct
    34381 gccaaccgcg cagcaatttt gtcagcgact tcctcatcgg acagggattt cgctgacaca
    34441 ccggaggcac cctgggtttc gcgtgacccg ggacttttga actgccaact gtcctgctga
    34501 aacagcccca gcaccacacc caatgaacga aactcattcc gcacagcatg ccgcatctca
    34561 gcagacagtc ctgcatcaag ggccttgttc gcgtctccgc gcaacttctg taatgccgcc
    34621 acagcattcg gcgtattcac atcatcgtcc atggcgtccg caaacgcctg ccgtgttcga
    34681 atgctggcct cgcgcatctg ctgatcggct gctccatgag aaggagccgg ttctttcaat
    34741 cgctcgaaga gatcatacag tccattgagg gcgttcttgg cttccgcgag gctttgatca
    34801 gaaaagtcca aggggccgcg atagtgcgtg gacaggagaa agtatcgcaa catctcgccg
    34861 gtcaccgcgt caggccattc agacttgtca aaaatctcgc gaatggtgaa gaaatttccc
    34921 aacgacttgg acatcttctc ttggttgatc tgcacaaagc cgttatgcac ccagtagcgc
    34981 gcgaattctt ttccggtcgc gccgcaggat tgagcaatct cgttttcgtg gtgtggaaaa
    35041 ataagatcca tcccgccgcc atgaatgtcg aacgtttcac caaggtgccg catcgccatt
    35101 gccgagcatt caatatgcca gcctggacgc ccaggacccc acgggctgtt ccatgacggt
    35161 tcaccgggtt tactgccttt ccacaaggca aaatccatgg ggtgacgttt gcgctcgtct
    35221 acatccacgc gcgcgccggc ctgcaaatcg tcagtgtttc gcttggaaag acggccatat
    35281 cccggatacc ggtcgacttg aaaatacacg tctccgttga cgcgataagc cattcctttg
    35341 gccaccaagg tatcaacgag ggcgacaatg tcggcaatgt gctcggtggc tctgggctca
    35401 agggtggccc gtcgaactcc gagcctctcc atatcgtcgt aatacgcctt gatgtaggtc
    35461 gccgtgatct ggtcacaact gaccccttgc tcattcgctc gcttgatgat tttgtcatca
    35521 acatcggtga agttcttggc aaattccacg gcaaagccgg agtattccaa ataccgccgc
    35581 agcacatcaa agaccaacgc gctccgggca tgtccgatat gacaatagtc gtagacggtc
    35641 actccgcaca cgtacatgcg gaccttgcct gggaccatcg actcgaacgg ttccttactt
    35701 ccggtcaatg tattatagat gcgcaacatg ttgcgtcctt actccataga cgtgcggcgc
    35761 ctgggccaat gtgaccagag gaaataaccg aggcccatgg ccagcaacac accgatcacc
    35821 agatccgcat gatggaaata ctggtgtaat tcattccagt gctctcccat cttcaatccc
    35881 acataggcga gcgcataaca gaacggaagc gctcccacaa atgaatagac gacaaacttt
    35941 ttgacgtcca tgcgcgaaat gcccgccggg agcgagatga acgttcgcac caccggcaag
    36001 agccggccga tcaacaccgc cgcctctcca tgtttcgcaa accaccggtc ggccaattcc
    36061 atgtcatgtg aggagacgag aaaatagcgg ccgtaccgct cgacaaaggg acgccccccc
    36121 cacacgccga catagtacgc tactaaggat cccagaacgt taccgatcgc accggccagg
    36181 gtgacgccca ccatcgaaaa ttggccggtg aacaccaagt agccggagaa cggcataatg
    36241 atctcacttg gcaggggaat acaggcactt tcgattgcca tcgtcaggac gatgccggtg
    36301 tagccgaagg tcgaaatggt cgcaatgacg aaccggccca atacttcgat caaccactcg
    36361 atcagagcgc tcacgcgcga gtcctcctcg aagaacgcgc tttgaccacc cgacggcttg
    36421 ccgccagctt agagagcggt tgctcccagg ccttaaagtg ttccagcatc gcgtgatagg
    36481 tcgtatccag atggatcggc gtgacggaca ccatacggcg ctctaaggct tcatggtcgg
    36541 cgtcttgttg acggctccac gattgacgcg tccctgcgat ccaataatat ttgcgccctc
    36601 tgggatccac tttctccacg atgggattgt tgaatcgacg tcgactcaaa cacgtcacct
    36661 tcacgccctt gatggaacga aagggaacat cggggatatt cacgttcaaa atggtttccg
    36721 ccggcaatcc atgctgcaac acttgtgcag ccactcgagt cgcataccgt gccccaacct
    36781 cgaaccggaa cgtctctccg ccttcctgcg ataccgctac cgatggtatg ccgagaattg
    36841 tcccttccag cgctcccgac accgtgccgg aatacatgac atcatcgccg agattgacgc
    36901 cgcgattgat tcccgagagg atcaacgcgg gagggccagg caaaattttc accaacgcga
    36961 gattgacaca gtctacggga gtcccattca ccataaatac gcgcggcgcc attttggtaa
    37021 tgcggagcgg cttgtgcaag gtgaccgcat gtcccaccgc cgttcgttca cggtccggtg
    37081 cgacaatcca cacttctccc agggtgccaa gcgcagccgc aacggcgtga atgccaggag
    37141 aggcaattcc gtcgtcgttg gttacaagaa tccgtacgcg cgccacagac actccttatg
    37201 catggaactc atcgtgaacg ggaagaactg aaatcgctgg ttcaagcggg gcgggaagaa
    37261 ggcgagaagc ttcacaaata cgtgaagatg gtcggtcgta atgatcaacg tctcttagaa
    37321 cggtacctca tggactacat cgtgccgatg gtcggggcgg cgggatttga acccacgacc
    37381 cctcgcaccc caagcgagtg cgctaccggg ctgcgctacg ccccgacacg gccgacatcg
    37441 atgttaccca cacggttgga cgcagccgcc cgcatcaccg gaacagttca tgctgaatgt
    37501 gaatcgatca ttcggatgat cgcctggaga tcgcccctca cgcgttgtgc cagttctttt
    37561 ctcgacccgc tctttttgac ggttgcgcgt cctcggcggg cccagtaccg cttcacaccg
    37621 gctagcgtat gcccttcatc atagagcatg cgcttgatct ccagcaccgt atccacatca
    37681 cgctgcacat acaatcgctg attgccgcgg cttttcttgg gcttcaggaa ggtgaactcc
    37741 gactcccaaa accgcaagac ataagccggc aatttagaaa tcttgctgac ttcgccgatt
    37801 ttataaaaaa ccttacttcc cagtctggga tcattcccca tgaccggtcc tcatgctgcg
    37861 tgtgctcgtg gcaaggtagc gactaggaat tcacgtactt tttaaagacc tgactgggtc
    37921 gaaacgtcac cacgcgacgg ggggtaatgc cgatttcttc accggttcgc ggattacgtc
    37981 ctttacgggc ccctttactt cgaacgacaa aattcccaaa tcctgcaatc ttcactgcat
    38041 cacccttctg cagcactccc ttcagcagat tgagcacgag ttcgacgata tccgctgcat
    38101 cgtttttaga cactccaacc tgcttgaaga tttcgttcgc gatatccgcc tttctcatgc
    38161 ctttcccccc gccatccact agagtcgttc cttaggaagt gcttcttggt gtcgagcgtc
    38221 caagacaaag aatttcccca taagttacgt gaggttatct ggcctgtcaa ggattatatg
    38281 cgccgaatgg agggaaaacg gcttcagcga ccggctagca gtaatttgta ctcgatacta
    38341 tccgccaacg cctgccaact ggcttccata atattttcag agaccccgac cgtcccccac
    38401 ttgtccttat gatcccccga ttcaatcagt acccgtactt tggattcggt ccccttatct
    38461 gcagacaaca cgcggacctt atagtccagc agtttgactt cacgcagttg cgggtaaaat
    38521 ttctccaagg ctttgcgtaa tgcatggtcg agtgcattca caggtccggc ccccaccgcc
    38581 gccgtatgtt cgacaacatc tccgaccttc accatgaccg tcgcttccga cagcagaagc
    38641 ccatgttcct gcttcttttc gacgatgacc ctgaagccca gcaactgaaa tgacggacga
    38701 tggctcccca tcgctttgcg catcaacaac tcaaaggagc cctcggcgcc ttcgaattga
    38761 tagccctgac tttcgcgctc cttgagcgtc tcgacgagtt cctggagctt cgcatgattt
    38821 ttggtcaacg aaatcccgta ggcttcaacc ttttccatca acccgctacg tcccgcataa
    38881 tcggaaacca ggatccggcg ccggttccct acaacggcag gatcgatgtg ttcataggtc
    38941 gcggcattct tgaggactgc atgaatgtgc acgccaccct tgtgcgcaaa cgcggcatcc
    39001 ccgacatagg gctgccgctt gttgggcatt aagttggcaa tctccgtgac aaatcccgag
    39061 acatctttga gtcgcgtcag tttgtttccg agggccggac gctgcatttt caattgcaga
    39121 ttcgggatga tggagcagag gttggcattc ccgcaccgct cgccgattcc gttaatcgtg
    39181 ccctgcacct ggagaattcc agactccacc gcaacgagtg aattggccac ggccatttcg
    39241 ctgtcattgt gcgcgtgaat gcccagtggc accggacact cctgctttac gatccggcaa
    39301 atttcccgaa tctcccacgg catcgtgccg ccgttcgtgt cgcacaaaat cactcgctcg
    39361 gcaccgccgg caatcgcacg acgaatggtc tgcagcgcat aatcaggatt ggccttgtac
    39421 ccgtcgaaga aatgttccgc gtcatagaac acgcgccggt ctttcgaacg cagatagtga
    39481 atggaatctt cgatgagttc taaatttttg gcgagcgcaa tgcccagagc atccgtcact
    39541 tggaacgacc aacttttccc gaagagcgta atggtctgcg tgccggaatc gagcagcgcc
    39601 tgaaggtttt tgtctttccg gaccggattg ctggctttcc gtgtcgagcc gaacgccacg
    39661 accgtcgcat tccgaaacgg catggttttg atctgccgga agaactcaat gtctttggga
    39721 ttggctccag gccatccgcc ctcgatgaaa tgcacaccga gttcatcaag ctgttgcgcc
    39781 acgcgaagct tatcttccac ggagaaactg acgtcttccg cctgcgctcc atcacgcagc
    39841 gtcgtatcgt agatctccag tgcagaggct tgtgcggcgg tgagcgtcgg tgcggtagtc
    39901 gagtccggag cggaagaccg gcgcgatgct gtcgttcggc gtttcataga tgtgcgagcc
    39961 acacggcggt tcgtgacgtg tcgagaaggc tgctcggcca gcgacgttcc cctcgcgaca
    40021 attagcccaa ggccggcgcc gacacgcgat ccaggccaaa cgccgtatgc agcgtgcgca
    40081 tggccaattc taagtacttt tcttcgatca cacaggagat tttaatttcc gatgtgctga
    40141 tcatcatgat gttgacgcct tcgcgcgaca gcacctcaaa catttttgcc gccactccgg
    40201 aatgggatcg catgcccacg ccaatcagag acaccttggc aatagattca gtgacggcaa
    40261 ccgaccgcgc cccgatctcc tgcgacaacc gctgaacgag atcgacggcg ttgcgcaaat
    40321 ccgccttcgg caccgtgaag gagatatcgg tcatggaggc ctggctgacg ttttggataa
    40381 tcatatccac cacgatattc gcattcgcca cggctccaaa gacacgcgca gcaatacccg
    40441 gacgatccgg cacaccgaca atcgtaagct tggcctgatt gcggtctccc gtcacaccgg
    40501 acaccatgac cccttccata tcggcatctt cacgtgtcac gagcgttccc tttccttcct
    40561 tgaagcttga attgacttcc accggaacgg aatatttcgc tgcgaattca actgagcggc
    40621 tctgcagcac ctttgcgccg aggctcgcca tttccagcat ctcttcgtac gaaatcttgt
    40681 cgagtcgacg cgcagccggg acgatgttgg gatcggcagt gtacaccccg tcaacatccg
    40741 tataaatgat gcagcgatcc gctttgaggg ccgcagctaa cgccacagca gtcaaatcgg
    40801 accctccccg tcccaaggtc gtgacatccg agctggcgtt aatgccctga aatcccgcga
    40861 ccaccggaat gacccctgcg gcgagtgctt ccttgattcg ttcggccgtg acacgggaaa
    40921 ttctcgcctt ggtatgagcg ctgtcggtat gaatgccgac ctgtcggcct gtgaacgatc
    40981 gggcgttcac gcctcgcccc cgaagctcca tggccaacaa ggcgatagtc acccgctctc
    41041 ctgtcgacag cagcatgtcc aattcacgct catccgggga atgggtcgct tcatgcgcca
    41101 gcttaagcaa tcgatccgtt tcgccgctca tggccgacaa caccacgacc acctgatgcc
    41161 cgtctttttg cgcgcgctcg acccgttcgg cgacgcgatg aatccgctct atgtttccga
    41221 ccgacgtgcc gccgtatttc tgaacgatca gtgccacaac ccttaggctc ctcgaggaaa
    41281 taatcgcatc atgagtttcg tcgcatcgcg aggagcggat ccggcgatgg tcttgtcctg
    41341 ttcatgaaaa cgggtggacg agccagccgg tggttgggtg gcaccgtcac acaacgcata
    41401 caaaatcggc ggaacgtccg agccttgccc ctttgccaga ccgggatcgc acaccacgag
    41461 cagacggtac gccggatact tcgctaacgc cgaaagaatc ggtccgatca cctgggcgtc
    41521 gaaacgttca atcgcctgta ccttgtcatg cacattggtc gcgtgcagca catcatccga
    41581 cagaccggca tggagataga caaaatcttt cttcgccaat tcctggacga ctgcctttac
    41641 acagccagca aacgtttctg attcatcgcc actctcggcc ggcagctcgg ccgcatccaa
    41701 tccggcacag aggccaacgc cgcggtgcac atcactggct gataccacca ctccggagac
    41761 ctgataccgt tccggcagcg gcggccacaa cggagcgcgc ccctgccccc ataaccacaa
    41821 acaattggcc ggcttcagcc cgtcttcttc acgctcctca ttgaccgggt gatcacgcag
    41881 gataaaaaag gaggcatcca tgattttccg cagcacgtct gcgccatcac cggccggcag
    41941 agcctcggcg atcgattgtc cgacgagctg ctgaggatcg acgcaggtcg cacgggattt
    42001 ccctccgacc cataccatta aatgccgatg acctgagccg gggtagaatt ggatactttc
    42061 cgatcccaat tgttcgttga cagcctcaac taactcgcga gcctgctccg tctcgatgag
    42121 cccggccgtc gcatcttcca tgaccacatg cggtcccagc ttcttgatgt cagccgcacg
    42181 gtctgttccg ccggaggcgg actcgcctcg caccgtgacc atcgtgcaac ggaacaccac
    42241 gtcctgctca cccaccgtca ctccgagacc ggcagcctcc aggggacctg gccccggata
    42301 atattttttg ggatcatacc ccaacaccgc gacaccggtg acatcgctgc ccgtcctgcc
    42361 tgcatcggaa ggaaggctca acagccccac ttctccgcgt tgggcaattt ggtcaagaaa
    42421 gggggtcttg gcggcctgga gaggcgtgct cccaccgagt tccgcacagg caaggtctgc
    42481 catcccgtcg gcatgcagga tcaagtattt catgcaatgt tcctgaggta atcagacatg
    42541 aaggaggcgg gccacatcat cgcgggtggc tttcaccgtt tgaggctgac gtgaaatgcc
    42601 gatcgccgta tcggcatcct tcaagccgtg cccggtcaac gtacagacaa ccgtggcacc
    42661 ttcttttaat tgtccggctt ggctcaactt aatcacaccg gccacggagg tggcagatgc
    42721 cggttcacaa aagacgcctt cttccctcgc gaccaaagca taggcatgga ggatttcttc
    42781 atccgtgacc atatcgatgg caccggacga ttccttcacc gccgtaaggg ccgactgcca
    42841 gctggccggg ttaccgatcc gtatcgctgt cgccacggtc tgcggctctt tcacgacatg
    42901 ccctaacaca atcggcgcgg caccggcggc ttgaaacccc atcatgcgag gcaaccgcgt
    42961 cgtttgattg gcggcacggt actcgcgata accattccaa taggcggtga tattacccgc
    43021 attccctaca ggaagtacat ggacggtggg cgcatcaccg agatcgtcgc aaatctccat
    43081 cgcggccgtt ttttgacctt caatccggta cggattgagc gagttcacca gctccaccgg
    43141 ataggtcgcc gataattcct tgacgatggt caacgcctgg tcgaaattcc cttcaacctg
    43201 gatgaccgtg gcctgatgca tcatggcctg cgataactta ccgagggcga ttttcccagc
    43261 tggaatcagc acatagacgg caatgccagc ccgagcgcca taggcggcgg ccgatgccga
    43321 cgtgttgccg gtggaagcgc agatgaccgc tcttgcccct ccttcgacag ctttggaaat
    43381 ggccatcgtc atgccgcgat ctttgaacga tcctgtcgga ttcattccct cgtatttgag
    43441 gtaaagatcc acacccgggg caattttttt ggccagccgt gtcgcgcgga tgagtggcgt
    43501 attgccctct cccagcgtga tgaccggcgt ctgagccgtc accggcagga acttccgata
    43561 ttcttcgata atgccgcgcc agcgagtcat aacgatctag cctgccacct tctcattgag
    43621 gtcggaaaat gccccactca ttcgtctttc ccctcaaccc tgatcaatgt cgtcggttct
    43681 gagatgtagg gcatgggatt gatctcgcga agagcatgtt ggatatcacg ctctttcgcc
    43741 atatgcgtca tgatgacgac cggcacggtc tgcccttcct tgcgtccctg ctgcagcacc
    43801 gaggaaatgc tgatgctgta gcgacccaat acccccgcga tctgagacag gacaccgggc
    43861 cgatccaaaa ccatgaagcg aatgtaatat aacgaagtga tttcttccat tggccggata
    43921 cgcagcggcc ttcgatagtc cggctgatag gaggctggcg gaacccggcc cgtcgcatcc
    43981 ttgagtaggt cgcgggcgat ggacatcaca tcgctgacga ccgcgcttcc cgttggcatc
    44041 gatccggcgc cacgcccata caagacgacg tcttcgacgg catcccccac caactgaatg
    44101 gcattgtaca ccccatcgac tttcgcaatt tgcgacgccg acggcaccat agtgggatgg
    44161 acgcgtgctt caatttctcc atcggaacac ttcgcaatcg ccagcagctt gatggtaaag
    44221 ccaaactcct tggcataggc gatatccagc ggggtaaggc cggtgatgcc ttcggtataa
    44281 atctccttga agttcaccgg agtgccgtaa gccaggctca ccatgattgt cagcttatgg
    44341 gccgaatcaa tacccgcgac atcgaacgtg gggtctgcct cggcataacc ggctcgtttg
    44401 gcctcatcta acacgacctc gaaccgctgt ccttcgctgg tcatccggct cagaatataa
    44461 ttcgacgtcc cattgatgat gccataaatg gattgaatat tgttcgcggc caaaccttcc
    44521 atcaaggcgc ggatcacagg gatgcctccc cccacgctcg cctcgaatcc aaggtcgatc
    44581 ccgcgacgcg acgcggcagc gaaaatctct tctccatgca ccgctaagag cgctttgttc
    44641 gccgtcacaa catgcttgcc tcgttgcacc gcatccagaa tcacgcgttt cgcgaggtca
    44701 tacccgccca tgagctcaat gacaatatcg acgcgcgggt cttccagcac ttgctggata
    44761 tccgtggtca atacacctga aggaatcgcc acgccccggt cacgggtaat gtccaaatcc
    44821 gcaatccgca ccaacttgat gggcacgccc acccgccgac ggatcaattc cgcattctca
    44881 atcagcactc gagccacgcc ggtcccgacg gtaccgaagc cgatcagtcc tactccgatc
    44941 tcagtcttca tggttcctga tcatccaaat tcaacacttt ccgaatcccg cgcaacgcct
    45001 gccgtgtgcg gtgctcgttt tccacaaggg caaaccggac gaattcatcg ccgccttcgc
    45061 cgaagccgat gcccggcgat accgcaacct tcgcttcacg gagcagcaat tttgaaaact
    45121 ccagcgaccc catgtgccga aacggatccg gaatacgagc ccatacgaac atcgtcgccc
    45181 gaggcaacgc caccggccaa ccgatacgat tcagcccgtt caccaggaca ttccggcggt
    45241 tttgataccg ttgcacaacg tctttcacac agtcctgcgg tccattcaat gctaccgtac
    45301 tggcgatttg aatcggctgg aaaataccgt agtcgagata gctcttcagc ttcgcgagtg
    45361 cgcctaccac ttcacggtta ccgacacaaa acccgacgcg ccagcccggc atattgtagg
    45421 ccttcgaaag cgtataaaac tcgacgccca catccttcgc ctccggcacc tgcagaaaac
    45481 tcggcgcctt atagccgtcg aaggcgatat ccgcgtaggc caaatcatga atcacgatca
    45541 catcgtgctc tttggcaaac gcgacaatct tcttaaagaa ctcgagatcg accacggcgg
    45601 tcgtggggtt gtgcgggaaa ttgatcacca gaatacgcgg acgcggcaac gtttggcgat
    45661 agacccgcag tagatcgtcg aagaaatcgc tgtcctgacg cagctcgatg ccacgcacct
    45721 cgccacccgc gataatgaaa ctgtacatgt ggatgggata ggtcggagtc ggcgtcagga
    45781 ccacgtcgcc cggcccgatg gtggccaaag ccaagtgtgc gagaccttcc ttcgagccga
    45841 tcgtgacaat cgcttcggtc tcgggatcca aatccacatc gtagttacgc ttgtaccagc
    45901 cagtgatcgc gtgacgcagt ttggtaatgc cacgcgaggc tgaataacgg tgattcttgg
    45961 ctttcctgga cgcctcgatc agtttgtcga caatatgggg cggcgtgggc tgatccggat
    46021 tgcccatccc gaagtcgatg atgtcctcgc cccgttgacg cgcctcgagt ttcagggatt
    46081 gcacctgggc aaatacgtac ggaggaagtc gtttgatgcg gtaaaaaccg tcgctgatcg
    46141 acatggatgt gtcagactcc tactggcggg gcctctgctg aacgggttgc gtcgtgacgc
    46201 ctctgaaagg agcgtatttt aatagagccc tcaaaacgag tcaatttcac tatatttcta
    46261 tggagttgcg gcttgccgct cggccatgaa cgtgagaaaa gcgcggcagc gccttgacca
    46321 gagccttctt gccccgcccc actgtcttct gttaataatg cctcaggaac aacagaattg
    46381 tctatgaatt tcgaccagtt ccgaaatctt tctgcatcgt cattcatggt atttgcggag
    46441 gcgtaatggc tcgattgggc gtgaacatcg atcatgtggc aacactccgg caagcgcgtg
    46501 gcggaaccga ccccgaccct ctgactgccg ccatcctcgt cgaactggcc ggcgcagacg
    46561 gtctcgtcgt gcatctccgg gaggatcgcc ggcatattca agaccgcgat ctgacgatat
    46621 tgcgggaaat cgttcgcact aaactagatc tggaaatggc cgccgatgac gccatggcca
    46681 aaatcgcgct aagtgtgaaa cctgacctcg tcacgctggt tccggagcgt cgtcaagagc
    46741 tgactacgga gggcggcctc gatgtcgcca atcaccgcga gcgaatccaa aagatcgtcg
    46801 atttgctccg cgatggcggg atcccgacca gtctgttcat cgaacctagc ttggatcaaa
    46861 tcaaggccgc tcataaaatc ggcgcggcct atgtcgagct tcacacgggc cgttacgcga
    46921 acgcaaaccg ctcgaaagaa gaagacgaag aattcgaagc gatcactcag gcggcaaaac
    46981 tagcctacaa acttggcctc ggagtgaacg ccggccatgg cctgacctat aagaacgtca
    47041 aacgactcgc caaactgccg gagatcgtcg agttcaatat cggccacagc attatcgccc
    47101 gctccgtcat ggtggggctc gttcaggctg tccgtgaaat gaaagaattg gtggcttagc
    47161 ctggctttcc ccaggcaagg tcctccctca gtcccgaatc ggaggcccga ttgacgtcat
    47221 gataccgatc gtgaccgccg agcaaatgcg tgcactggac catcggacga tcaccgaggc
    47281 acaggtaccg tccctcgtac tgatggaacg agccggaaca ggtgtggtga ctcatcttga
    47341 ggcgcgatac ggatcggttg caggaaaaaa gatcgtcatc ctgtgtggca agggcaataa
    47401 cggtggagat ggtttcgtcg tcgctcgact gttacggaga aaaaaagcta gagtccacgt
    47461 gttgttatta gcaccggtgg ctgaactcag ccgcgatgcg aaggcgatgt atcagcgctt
    47521 tcaccggaca gcaggcccat cttccattac tcgcccctct gatacgaacg ccattcaaca
    47581 acggctgaca ccaagcgaga tcattatcga tgccatttta ggcaccggcc tctccacgcc
    47641 tgtgaccggc ttataccgtg atgcgataga cgccatcaat gccgctggac gcctcaccgt
    47701 cgccgtggat cttccgtcag gccttcatgc cgataccgga tctgtcctgg gcacggcggt
    47761 caaagcggac ctcaccgtga cctttggatt gccaaaggca ggactctatt gcggaggcgg
    47821 catcgattgc gctgggttgg tgcgtcttgc tgacatcgga attccgtctt cgtttgtcga
    47881 cgccgtcggc agtcggctgc tgctcctgac cgctgcctcc gcgaacgcgg cgcttccccc
    47941 gcgacacccg tcctcgcata aaggcaccta tggacatctc ggcatcatcg ccggatcggt
    48001 cggcaaaacc ggagccgcgg ccatggcagc catggccgcc cttcgaatcg gaaccggtct
    48061 cgtcacggct gccgttccct cgagcgtcaa tgacatcctc gaggcgaaat tactcgaagc
    48121 catgaccctg ccgatgccgg agaccaaggc ccgcaccttt gcccgttcag gactggatcg
    48181 cctcctggca ttcgcgggtc ctcgcgatgc cgtcgcgatc ggacctggac tgacgacgca
    48241 tcccgagacg gtggacttgg tacaagaatt cgtgaagcgc atcgataagc cgtgcgtgct
    48301 cgatgccgat gcgcttaatg ccttggccgg aaaggcgtcc ttgctcacgg aatgccctcg
    48361 cccgccgatt attacgcccc atcccggtga aatggcgagg cttgaggcag aagccaccac
    48421 ccagtccgtc aatgaagatc gcctcgggac agcaacgcgc ttcgcacgtg aacgaggcgt
    48481 gttcgtcgtt ctcaaaggag cccggaccgt catcgccaga ccggacgggg tcgcagcgat
    48541 ctgcccaacc ggtaacccag gcatggccac ggcgggaacc ggagatgtgt tgaccggcat
    48601 ggtcggcggg ttgctcgccc aaggtgtggc tccatgggac gccgcctgtg ccgccaccta
    48661 ttttcatggc cttgccggag atctcgccgc gcagcaactc ggccaggccg gcatgattgc
    48721 ccgcgatctg attcgccata tcccccatgc catcgcgcca accacgcacc tctaagctgt
    48781 tgaccacttc acctgcagga gggaagaagg caaggcctcc cgctgtcacc aaaccctgga
    48841 aaacgctcct tcgctcccgg cagcaaactc atcggctagg ccgatgtctg ggcaccctcc
    48901 ttgagggagg cgaagttgtg gccctgttcg gagaaatggg tactgggaaa acgtcactgg
    48961 ttcgaggcat cgccgatgga cttggcgccg aacccatggc tgtgagtagt ccgaccttta
    49021 ccttaattca cgaataccgt ggccgccttc ctctgatcca tactgattta taccgcctca
    49081 gtgccgccca actcgagaat acgggcctgg gtgactacgt agacggccgc gctattctcg
    49141 ctgttgaatg ggccgaacgc tggggcgcag ggcttccgca tgatcggctt gagatttatc
    49201 ttacccatca ccccccgtcc acgcgacagg cggtgctcac agccagaggc ccgtctgcat
    49261 cacgcctgct cctcgccctt cgctctcgca tgtccgtgcg tcgtcccact agagtggttt
    49321 cgcaaacccg tgtacaatga ccctcggcca gggagggcct cgcattgatc cgcttgatat
    49381 tggtagggac ggtactgctg ctggcgctgt cgttctttct tcagaatcaa gaacaggagg
    49441 tcactctccg gtatttcttc ggccttcgat cggcgtcaat tctcatctat aagccgatcc
    49501 tggcggcctt cggcgtcggc ctgctggtct ccggcattct gctttttccc gcctgggtcc
    49561 gcgggcgaat cgagctcaga cgaaaaacca aggccttgca ggaagcggaa gtcgatctcg
    49621 aacggctgcg ccaggcgctc gacaaagccg cccgccgcgt tggaagctct tctgacatcc
    49681 ctacggaggg agaacccgtc gatgggtgag gcagacggca cgccgttgat gcggcagtac
    49741 cgcgagatca aacaggccta ccgtgatgct attctgttct tccgggtcgg cgacttctat
    49801 gaaatgtttc aggaagatgc ggtcgaagcc tccaagcttc tttcgattgc gctgacctcc
    49861 cgcgataaat ccagcgaaac tccgatcccg ctctgcggcg tcccctatca cgccgcaaca
    49921 aattatatcg ccaagctctt gcgcgcaggc cgaacggtgg ccttgtgcga acaagtcgaa
    49981 gacccgaaac ttgccaaggg tctggtcaga cgggaggtcg tccggctcta tacgcccggc
    50041 acactgatcg atagcgaatt cctcccctca tcggaatcca atctgttggc agccgtggcc
    50101 atccgcttgc ggcctgcgac acacgaacct acaggctgtt cattcggtct cgcgacgcta
    50161 gaggtttcca cgggagagta ctggctttgc gagtttcatg gtccgcaagc gcagatcgca
    50221 ctcatggacg aactcgcacg gcttgacccg aaagagttgc tgtatgccga agggtgcaca
    50281 ccgggagcac aatcctggat agctgaatta atcggacctc gctgttgcgc acaaccggca
    50341 acatggtttg agctggatac cggtcgcgca atgctgcagg aacacttccg tgtgcactcg
    50401 ctagactcct tcgggtgcca gcagctcaca ttgggtattc aagccggagc ggccgtgctg
    50461 cgctatgtga gggagaccca accttcggcc tcactcgacc atattcggaa cttgcgagtg
    50521 agacagcctc acgacgccat gcatctggac agtgtcacga tccgaaattt ggaactcgtc
    50581 aaacccgcag gacgctggga cgacgcgccg aatcaccgct caccgtatac attgttaggt
    50641 gtcctcgatc gcacggtcac ggcaatggga agccgattgc tccgcgaatg gcttctgcgc
    50701 ccactaatca actgcgcccc gattgaagcg cgcctcacag cggtcgagga actcaaagag
    50761 agcatcgagc cacgagttcg attgcgaacg gcgctacgca ccgtccagga tatttcgcga
    50821 ctctgcagcc gaatctcgct cggcgtcgcg aatccacgcg acttgctggc cttgaaactc
    50881 tcgttatcct ccctgcctgc cattcaggcc gaactcacgc cgttgcaggc cccgcttctc
    50941 gccgagctct cagcatcctg ggacaacgcg caggacttgt atgaactgat tgaacaagcc
    51001 attcttgccg acgctccagt ttctatacgc gacgggggca ttttgaagga cgggtatcac
    51061 ccggatgtgg acgagcttcg caaagccact cgtgaaggga aaggttggat tgcaggactg
    51121 gaggccaagg aacgggagcg gaccggcgcc gagtctctga aaattcggta caaccaggtc
    51181 ttcggatact atatcgaatt gacgaaagcc aatctcggca aggttccgtc tgactatatt
    51241 cgtaagcaga cactggtgaa tgccgagcga tttatgacgg ctgagctaaa ggaactggag
    51301 gaacgggtca ccggtgccga tgccaaactc gtggctctcg agcaatcgct tttcgaacag
    51361 ctcagaacag gcctcgccgc cgaaacagcc cgcctgcaag agatcagtcg cagactggcg
    51421 gttctggatg tcctcacagc gttggcggaa acggctgggt tgaatcgtta cgtgcgaccc
    51481 accatcgaca cgggcgatgt actccacatc aagcaaggcc gtcaccccgt cgtcgaacga
    51541 cttgatctct ccggaggatt tatccccaac gacacgcacc tggatctgca caccagccgc
    51601 cttcacattt tgacaggccc caatatggcg gggaaaagca cctacctcag gcaggtggca
    51661 ctcattgcgt taatggcaca gatcggcagt ttcgtccctg cagccgaggc cagaataggg
    51721 ttggtcgatc gcatctttac ccgggtcggg gcatcggaca atctcgcggg gggccaaagt
    51781 acctttatgg tggagatgac cgagtctgct catattcttc actgtgcgac cgcacgcagc
    51841 ttgattttgc tggatgaaat cggccgtggg acaagcacct acgacgggtt gagcattgcc
    51901 tgggccattg cggagttcat ccaggatccg cagcgactcg gagctcgcac gctcttcgca
    51961 actcactatc atgaaatgac tcagctggag ggattacgag caggaatcac gaattattct
    52021 gtggcggtga aggagcgcga cgggaaagtg ttatttctgc gcaagatcgt ccaaggaggc
    52081 gctgatcgta gttacggtat ccatgttgcc cagctggcgg gccttccgga cgcggtcata
    52141 cagcgagcca aggctgtact agcacaactg gaatccacaa cagcaccacg tcccattctt
    52201 gaaagtcagc aagctctaca gtttgattcc actctccctg ccccgcatcc catgctggaa
    52261 gaagtccgcc aaatggacct cttctccatg acacccctcg atgccctcaa tcggctggct
    52321 gcactccaac aacggctttt gagcgagtaa cgattccgca tcgagcggct tcgacttcag
    52381 ttcactccgg ccaaagaaaa aggcgacacc cctttcggag tgccgccttc ttcatgagcg
    52441 cataagtcgc gagaacttag tgcttgctgc cctgattgta cttggcggtc caaggaatca
    52501 gattggcaat tggcttgcca tcaggcaaca agacatcata ctgcaggggc aaattggctc
    52561 catccggagt cttcggcgac gtgttcaaca acgtctttgc ggcatggcaa gcagcatcca
    52621 cttccgtctt gccttcacat tccttcaaac gaagggatga aatgctcttc ggcttgccca
    52681 tttcgccggc gttgtcattg agcttcttca cggatgtgat cacccggtga ttctgctcgg
    52741 tttcctgggc cacaaactcc actaaggtcg tcggggatcc aggaggaact tcctttgccg
    52801 cggccggtcc ggaatgcgca cgggccatgg acttcagctg ttcccacacg ttcttatccg
    52861 caggatagaa cttgaggttc tttccaggat ggactctctg ccaccacaat ccgctttcgg
    52921 cgttgccacc gaacaccgcg atgttgagcc acacggcctc atcatacgga tccaagatga
    52981 tcactcggcc ctgcaacgtg gtgggcttgc tcagatctcc ccactcaaat ctctcctgga
    53041 acgcctcgat ggccagcgca gtggaggcaa aacttaccac caaagctgcg gcagccatga
    53101 aggtccaccc ttgcttttga agcttcatca tcacatcctc cttaaagaag acaaaagagt
    53161 ctcaagaatg tcgcctcgcg tataaaatct acttcaatac tttgaaaccg gtaatcgtgc
    53221 gaccgtcgag ggtcacttcc atcgcaacga gctcttggga agccttgatg atctgatcca
    53281 tcaagccctt gtccttcacg gccaaccgaa ccgtggtctg agcatggtgc caaccggcgt
    53341 acggattatt cttgtccgtg ccaccagtgt agccccaggc gcagcacgta aagagggggt
    53401 ccggcggctt cacatccatg gaggttgatg aacgaccacc accctcacct gaagcgggat
    53461 ctcccgtatt ccaatattga atgccgaaca aaatttcacc gtcgggattg tcggcccact
    53521 gcgaccacac gcgacccttc aaggtattcg tgggttcgcc tttgaaaaac ttaccactgc
    53581 ccgtggtgac ttcgctgggt ccgcccccgg ctggagcgtc ggccgccatt gagacaccgg
    53641 cggtcaacaa gccgacaacg gaggagactg caagtgcggc tagaattccg accctattca
    53701 taaccctacc ctccttggaa aaatgatgac cagagaaccg ttgaatcaca gtatccaaaa
    53761 aaagagttca aaaaaacaaa aaatgagaga acgcgctagc aggaggaagt gcgaaccaca
    53821 ccccctttct ctctgcttac cttcccctgt aatgcagtcg aaataaattc gaacgtatag
    53881 gatgaaaacg cggccatggt acttaaatcc accaaacgtg tcaagaaaat gtttttcgaa
    53941 aattgtacgg cagttgccac gatcaaaatg ccgcccgatc aatcagttag cggtcagaag
    54001 cacgtcgatg gttgcttctc tctcacgctc agttgctgcg ccgaccgagg agatgcaggc
    54061 gcgtgatcta ttatgacgca aacgactgaa ggctattcac cggtatgggt ccaagcgctg
    54121 tgatcgcgag acaccgctga tccaacaagg tctgggccac acgatagacc tgatccgtcg
    54181 tcacacggtc aatctccccg atcatttgtt cgagcgaaac atgactccct tgcgtcaatt
    54241 cgtctttcgc gagtttactc atccggctat gcgagctttc caggctgagc atgagactgc
    54301 ccttcatttg attcttcacg cgggcgagat cttttgcatc aatccccttg ctgcgcagct
    54361 tcttgagttc ccggcacacg acctcaacta cacgctccac ctctttcggg cgcgtgccgg
    54421 catacaccgt cgtcatcccg ccgtccgagt atgtggacag gaacgaataa attgaataca
    54481 cgagaccacg cttctctctc acctcctgaa acaggcggga actcacgctt ccgcccagca
    54541 ctccattgag cgcatgagcc gcataccgat ctttatgtcc agcgcccaat ccctgcaggc
    54601 ctaaacagag atggacctgc tccaacgcct tacgcctcac cagcactcct ccacggactt
    54661 cgggtggccg ccgactctgc ttgaccgcga cgcctttgtg aggggcggag aagtagcggg
    54721 ccaggagttg ttcgaggcgc ttccaggtga agttccccgc aacggcaaca accgtgcgct
    54781 caggatcgta gtgagaaccg acatacgaga ccaggtcctg acgacccaac gcctggattc
    54841 tcggagcttg ccccaggata ggccggccca acgggtgact gccgagcgta tgcttcatgt
    54901 ggagttcctg aacgagatcc tccggatcat cctgaaccat ccggatctct tccagcacca
    54961 cttgtttttc tttttcgact tccttggact cgaaccgcga acggtagaac agatcggaga
    55021 gcaactccaa cgccgcttcc aattgttgat cgagcacttt cacataaaac gtcgtcgtct
    55081 cgcgagtcgt gaaggcattc atctccccgc ccagcgcatc aatttcacgg gaaatctgcg
    55141 tcgccgatcg gcttcgcgtc cccttgaaaa acatatgctc gagaaagtga gacagtccct
    55201 cctcgcccgg ctgctcgtcg cgggagccga cattgaccca aatgccgatc gtgacagact
    55261 tcaacgtcgg cagtaattcg gccacgaccc gcaacttgtt gtcgagaacg atcttgcgat
    55321 acacgatcga ttatcctgcg ggagtcggct cagttgcggg agagcccgca ggtgccggca
    55381 tcgcttcttt ccggctcaaa cggattttcc cctgcttatc gatttccaac actttcacct
    55441 ggatctggtc accttccttg acctcatcgg tcaccgcctt cacgcggtgg tgcgccaact
    55501 gagagatgtg gaccaaaccg tcggtgcccg gaagcacttc gacgaaggcg ccgaaatcca
    55561 taatctttcg aacggtcccc agataaatct tgccgacctc gacttcttcg gtcagacgct
    55621 tgatcatatc gatggccttc tgtgccgagg cttcgtctga ggacgcgatc gtcacatcgc
    55681 ccgtgtcttc gacattgatc ttcacaccgg tctccgcgat gatgctgcgg atcatttttc
    55741 cacctgggcc gatgacatca cggatcttat cctgcttgat cttcatcggg aatatgcgcg
    55801 gagcgaaggc agacaacttg gccctgggag ccgtcaacgc ctgcgccatg cagcccagaa
    55861 tatgcagtcg accggccttg gcctgcgcca gcgcttcccg catcaacgcg gcgtcgattc
    55921 ccccgatctt gatgtccatc tgcagcgcgg tgactccgtt tttcgtcccc gtcaccttga
    55981 aatccatatc gcccagatga tcttccaagc cgagaatgtc tgagaggacc aagacctgat
    56041 caccttcctt gatcaggccc atcgcgatgc ccgccaccgg ttccttgatc ggcacacccg
    56101 catccagcaa ggccagcgtt ccgccgcaga ccgtggccat ggacgaggag ccgttggatt
    56161 caagaatttc cgacacgatt cgaacggtgt aggggaactt atctttcccg gggatgatcg
    56221 acttcaatgc gcgctctgct agtgccccgt gcccgacttc gcggcgccca ggggatcgca
    56281 acggcctagc ctcgccgaca ctgaagggcg ggaaattgta gtgcaacatg aaggtgcgca
    56341 tgtactcgcc ttccaaggcg tcgatacgct gctcatcgtc agtggttccc aacgtcacca
    56401 ccgctaagct ttgcgtttcc ccgcgggtaa ataccgcaga gccgtgcgcg cgtggcaaca
    56461 cactcacttc gcacgtgatc ggacgaatgt ccgacggacc gcgcccatcg gcacgcaccc
    56521 gtttctcgag aatcatgttg cgaacttcgg tgtattccaa gccgtggaaa atgattttga
    56581 catgccggtc gcggttgggc tcttccgact taagcgcgac aagcgtctcg gccaggacct
    56641 gatccaatcg ctcctgccgt gcgctcttat tgggaatcag tatggcttca cgaatcgggc
    56701 ccgccaccag cttgcgcact tgttcggcca aggcagcgtc gatcggttct tgcgtcacta
    56761 ctcgcttcgg cttaccggcc agggtgcgaa gctcttcgat cttcgcgaca atttttttaa
    56821 tctcgctgtg cgccaactcg atcgcctcca gcatggtcgc ttcaggcaat tcattggcgc
    56881 ccgcttcgac catcatcaca gcgtcggcag tgcccgccac cacgagatgt agatcgctcg
    56941 tctcaagggt ttccaagtcg ggattcacga caaactggcc gttcacccga ccgattttca
    57001 cacctgcgat ggggccattg aacggaatgg aggaaatggc caacgcggca gacgcggcgg
    57061 tgatcgcaat cacatcggac acgccggttt tatcagccga gagcaccgaa gtgatgactt
    57121 gggtttcgaa atagtagcct tcgggaaaga gggggcgcag cggacgatcg atcaatcggc
    57181 tggtgagcac ttcccgctcg gagggtcgcc cttcccgctt gaaatacccg ccgggaatct
    57241 tgccggccgc gtaggtcttt tcctggtaat ccaccgtcag cggaagaaag tccacaccag
    57301 gcttcgcgtt ttgtgaggcg acagccgtgg cgagcaccac cgtgtcgccg taggtggccc
    57361 aaatcgcacc gtccgcctgt ttggcaatac ggccggtctc cagtgtcaag cggcgacctg
    57421 ccagctcgat atctactact tgtttcatct atggtctcct cccgttaaat cccacagatg
    57481 cccacagaga taagctcaat tcgaatgcgg cccctagatg gcccatgcgt ttgaacttgt
    57541 ttcagtgttc acctgcgggg acaatagttc ggccgccaca catgtggcgg ccgattcgat
    57601 cacacgacta cttacggatc cccagacgtt cgatcacggc gcgatagcgg ccttcttcga
    57661 cgtgccggag ataatccaac aagcggcgac ggcgtccgac caacgtcaac aatccacgcc
    57721 gggaatggtg gtccttcttg tgcaatttaa aatgttccgt caaatacgta atccggttcg
    57781 tcagaatggc gatttgcact tccggagaac cggtgtcctt gtcatgctgc tgaaaattct
    57841 tcatcaactc tgtcttcact tctttgacca gtgccatacg tctctactcc tctattgagt
    57901 tcgcaatact actaccctgt gactcgtcct cgactaatac ctttgacatc gcaatcgaca
    57961 gctcgcccgg catcctggcc tggtcgccga gtccggcggg catggtgccg atcgcgagca
    58021 atcgtccagc cgtgtccttg atacggatgg gtccacccga ggcttccggg agaatggtcg
    58081 gcaagccctg ccaactcaac acatcggcgg tcggaactgc catgccatgt acgacctgcc
    58141 gtgcagtctc ccggccgatg atgcagactg gaagtcctac caacgcctca tccaacgtga
    58201 gcatcgatcc ggccaacgtc ccctgagtca ggcgtgtctc gacttcattg acggtcaagg
    58261 actgctctac catcagcggc ccgacgcgtt cgcgtatcaa ggtcgccata tgaccgccaa
    58321 ccccgagttt ttctccgata tccgcgcaga gcgtcctgat atacgttcct ttggagcaga
    58381 ccacacggag catgacatca ggcagctgaa tgctgacgac ttccaaacga aagacggtga
    58441 cttcacgagg ctggcgggcc acatccttcc ctgctcgcgc agctttatag aggggaaccc
    58501 ctcccacttt cacggctgaa tacatgggag gaacctgttg aatgcggcct tcaaacccag
    58561 caatagtttc ccggatacga gcttcggtca aagccgtaat aggcgagcgc tggagcaccg
    58621 ttccagtggc atcttgcgta tcggtcgttt cacccaatcg cagtccggcc agataggttt
    58681 tatcccattc gagcagatat tcagcgatgc gcgtgccacg gccgaccagc agcgggagaa
    58741 ccccggttgc agcgggatcg agggtgcccg catggcccag tttcattccg cgcaatttcc
    58801 ctcgaacacg ggcgaccaca tcgtgtgacg tccatccggc ttctttgcgg acattcagca
    58861 cgccatcctg aagcatggta gccgtctggg attccgtcga cgcgatcatc atcggctaga
    58921 catcggtgcg aggggtttct ggtagcctgt cttctttggt ttcatccagg tgcaatccgt
    58981 ccaacagcgc gagaacccga tcagccctgg ggccgctgac gtctttcata aaaatcaatt
    59041 ccggcagata ccgtaacgcc agtcgccgcc ctagttccgc ccgaacaaaa ccactcgctt
    59101 tcgctagtcc gtcaaatacc agccgctcgt ctttgttctg ctccatggtg gtgacaaaga
    59161 cacgcgcaat gcgcaaatct ttggtcaact ccacatcggt caccgtcacg gaacgcacgc
    59221 gcggatcttt gatcttgcgc attaatatgt cggccacttc catgcggatc tgatcggcca
    59281 cccgttcggc ccgactatac gtcgatttcg tcatgacgtg gccttacaac aattcgatcc
    59341 gagagcggag cagctgaatc gtgggcaccc cctgaatcaa attcacagcc tggtccagca
    59401 cctggttgac gtgggcagat tcattcgcca cacaggccaa tcccaaaatc gccttctgcc
    59461 acaattcctg ctctcctact tcagccactg acacgttgaa cttatcccgc aagcgacttt
    59521 tcaaactctg caaaacctgc cgcttgtctt tcaatgaatg tccatcgggg atgaacaact
    59581 cgaccgtgca aagcccgacg atcatgcccg atgaggttgc ggggaagtgc cgcggtgtgc
    59641 cgattcgagc tttgccgcga ccttgtcgat ggcaaaggcc tcgataatgt ctcctgcttt
    59701 gaggtcgttg aagttttcaa ccgtgatacc gcactcatag ccttgctgga cttcccgcac
    59761 atcgtccttg aaacgcctca gcgagcccag cttcccctcg tagaccacca cactatcccg
    59821 aatcacacgc acgccgacac tggcgcgcga gatgacgcca tccaccacgt agcaaccagc
    59881 caccaggccg gccttgggaa tcgtaaacat ctgccgcact tcggcacgtc cgagaatccg
    59941 ctccttgagc gtgggctcga gcaacccttc catagcggca cgaatatcat tcaatgcgtc
    60001 ataaataatg ctgtagagac ggacatctac accctctcgt tccgccaacg ccgcggcctt
    60061 tggctcagga cggatgttga atccgatgac aatcgccttc gacgcggccg ccagcaggat
    60121 atcggtttcg gtaatccctc cgactccggt gtgcatgacg cgcaatttga cggcgccggc
    60181 cggcattttc tcgacggcag cagccaaggc ttcggaggaa ccctgcacat cagccttgat
    60241 cacgatcggc agctctttga cattgccctc ttggattttg gcgaacaagt catccagact
    60301 gactttcgcc gggcctgcca gatcagcagc ccgctgcttc atcgctcgtt cttgcgcaat
    60361 ctcgcgggcg actcgctcat ccttcacgat ggtaaaggcg tcaccggcag agggcacacc
    60421 cggcaatccg atcacttcga ccggcaccga tggtcccgct tcggtggttt tctttcccgt
    60481 atccgtgact aacgcgcgaa cccggccgct gaaattaccg acgacaaacg cgtcgcctac
    60541 gtgcaaagtc ccgctttgca caaggaccgt cgcgatgggg ccgcgcccgc gatccagctt
    60601 ggcttcgatc accaagccct tcgccatgcg cgtggggtcc gctttcagct ccaagacttc
    60661 cgcctgcaag aggatcattt ccagaagttg atccagaccg gtccgctgtt tcgcggaaac
    60721 ttccaccata atcgtgtcac caccccacga ttcggccacc aacccatgct cggtcagggc
    60781 atttttcaca cgatcgacat tggcacccgg cttatcgact ttattgatgg cgacgatcag
    60841 cggtacgccg gcagccttgg catgatgaat ggcctcgact gtttggggca tgacgccatc
    60901 atctgcagca acgaccaaaa tgacgatgtc cgtcgccttc gctccccgtg cccgcatcgc
    60961 ggtgaacgct tcgtggccag gagtatcgag gaacgtgacc tgcttgtcat gaaccccgac
    61021 gatataggcg ccgatatgct gcgtaatccc tccggcttcg ccttcggcga ccttcgttga
    61081 acgaatcgcg tccagcagag acgtttttcc atgatcgaca tggcccatga tcgtgacgac
    61141 gggaggccgc gacaccgcgt gctcttcacc ggatgactgc gccgcctctt ccagcaaggc
    61201 ttcgccgact ttctccgtcg acacttccac cctggcgcca tactcttcgg caatcaacga
    61261 cgccgcctcg agattgatcg actggttgaa ggtcaccatc tgccccatat ccatcagctt
    61321 ccggaccacg tccgccggcc gctgaccgat gagttccgca aactctttga cgctgactcc
    61381 tgcggtcaat ttcacgcttt tctttctcgg cttggtgatt tccgttggcg aggcatggtg
    61441 aatatgtttg gaccggtcct cacgccgctg caccgggatc gcccgcaaat ctccccaccg
    61501 agtggcgtcg ttcttaaact tggcttcatc ttcttcccgg gtccggggcg ccttttttgc
    61561 ctttttcaat ttctcgcgcg cagcggcttc gctttctgat gcatcgggaa cgagactttt
    61621 cttcttgatt gcggcaaggg gatcgacagg aggagtcgtg accacaggag cctgagacgg
    61681 cttggtcgtc acatcttcgg acgtcgcgac aacgggcggc ggaggctcgg gctcctgggc
    61741 ctgaaccggt tcaatcggac cagcctcact cacttcagaa cctggcaggg gggcctgatc
    61801 cacaactccg gcgtctgccg ctgcgggagc agccggggca aacaccgctt ccgctgcggc
    61861 aagcgacgcc gcgacatctt ctgcgccctc ttcacgcttc ttcttgatga gaatgcgctt
    61921 cttgtcgggt ttcggcgcct cttcatgcgc ggctgcgtgc gatccagcgg actctttcgg
    61981 caaacgtgtc atcttcttcg cctcatgccc ggaagaagac gtctcaccgg cacgcgcctt
    62041 cgagccaagc ttttcaagcg ccatgcggac cgaatcgtca tccaacgcgc tgctgtggga
    62101 tgccaccgga atcccgagcc gcttcagttc aggaatcagc tcccgatttt ccatccccag
    62161 ctgctttgct aattcgtaca cgcgcataca gtaccgctca gctccacccg cttagataat
    62221 tgccattcag ctcgtgagtt gcgagccctt ttactccgct tgctgcgccg cagctcgtgc
    62281 ctcatcctgc tggcgctgag cctcggtctc ttcggccaag gccgccttaa tgtcccgatc
    62341 ccgctcggcc ttttccttct cgtattcagt cgcgctgatg atatcgatct tccaacccgt
    62401 cagacgcgcg gccagacgga cattttgtcc gttcttcccg atcgccagcg aaagctggga
    62461 atccgccacg accactagtg ccgacttctt ttcttcatcg atgccgactt tttcgatcgt
    62521 cgctggattt aacgcttccg cgatgaacac gcgaggatcc tgtgtccacg tgataatgtc
    62581 gattttttca ccacgcaact ctcgcaccac cgcctgcaca cgcgacccct tgatcccgac
    62641 acaggctccg accggatcca ccgctttgtc gcgagatgac accgcaatct tggtacgatc
    62701 acccggctca cgcacgatcg acttgatttc aacgattttc tcgccgactt ccgggacttc
    62761 cagctcgaac atcttcgcga caaactgagg atggctacgt gaaaggatga cctgcacgtc
    62821 cttcggtgtg cgacgcacct ccagcagcaa ggccttcacg cgatctcccc tccggtaggt
    62881 ttcgcgcggg atctgctcct gaatcggaag aatcgcctcc gtcttgccga gatcgaccag
    62941 gaagtttctc cgctccatgc ccagaatgat gccgttcacc aagtctccct gacgggtcga
    63001 atactccttt tgaacggctt cccattctgc ttcccggacc ttctgaaaaa tcacctgctt
    63061 ggccgtttga gcggcgatgc ggcccaattc gtccatttcg atgagggagc cgatttcatc
    63121 accgacttcc gcgccctcat catattcacg cgcctctttc agggaaatct cagccttcgg
    63181 attggccacc gtatcgacga tgattttctt cgacacgacc gaaatttcgc cggtcttgct
    63241 atcaatttcg acctggatat tctcggcctg gccgaatcgt ttcttcgcag cggtttgcag
    63301 cgccgactcg atcgcgccaa tgaccctggc cttatcgatt cccttttgac ggccgatttc
    63361 atcgatgacg gcgatcaact ctcggttcat agctcacacc tcatggttaa cagagcagcg
    63421 ggcaccgtct gctacttgga aaattcgacc ttccgacgcg ctacggcgat gtgatcaaac
    63481 tccacccgga tcgtctccgt cgtttttttc tgctggatag cgagcgtcac cgcgccatcc
    63541 tccacttcca gcacggtccc caccagccgc cactgcgact gaatcggctc gcgcaatttg
    63601 agcgtcaccg gctcgccgat caaacgccga taatcatgcg cggttcgcag ggggcgatcc
    63661 aggccgggcg acgaaatttc caacgtatag gcatgtggaa acgggtcgat gacatccagc
    63721 gccgggctca gagagcgatg cgcctgctca cagtcggtca gcgaaattcc gccgggcttg
    63781 tcgatgaaaa cccggatcac ggtccgggga ccttggccga cacataccgc ctcgaccaac
    63841 tcaaggccgt gggattgcag aatgggagcc gcaacttctt gcacgcggag gttgagcgcc
    63901 tcagcgcgtt tcaccaaagg cttccctgcc gacaccgctc ccgcatcact cataggcaaa
    63961 agttgctcaa acaaaaaagt gggcccaggc ccacttacag cggaggaacc ttaccatgcc
    64021 ataggagtga aagcaaggga cgctacccga ccagggcctc ccaacgacga gacaaacgtt
    64081 gtgacagcgg gcctggcaca cctgcaccga tgggttgccc gtcaatgcgc acaaccggca
    64141 acacttccac cgtcgttccc gtcaagaaca cctcggatgc cgccaagaga tcttcccgac
    64201 cgacaaatgt ctccaccacg ggaagccctt ccttcctggc cagctccagc accatcgccc
    64261 gcgtgacccc tgagagaatg cggtgccctt cgggcgcagt ctcaaccaca ccgtttcgca
    64321 caaccatgac attgctgaca gacccctcgg tgacaagccc atctcgaact aaaatcgcct
    64381 caaacacacc ggcttccttc gcgcgctgcc gcgccagcac attcgccaag agattgacgc
    64441 tcttgatatc gcaccgtccc cagcgaatgt catccactgt tacggctcca accccggcct
    64501 tgcgaattga ggcatccagc ggatggagtt cgcgaaaagt cagcacggtc gttggcacgg
    64561 tggaggcggg aaagagatga tcccgtggag cctgccctcg cgtgacctgc agatagattt
    64621 tggtctcagg gaactggctc agccgaaggc cttcctgaac gagcgccatc cactgatctt
    64681 tggtaagccc caacgagagg tgcaacgcct gcgcacttcg ctcaaaacgg gccaaatgct
    64741 catcgagggc aaatggtttc ccgcgatagg tgcgaatgac ctcatacaca ccgtcgccaa
    64801 actgaaatcc ccggtcctcg atgctgacta cggcttccgc cacaggaccg aagcaaccgt
    64861 tcacgcatgc aatgtcaggc atggcctttt tctcgccgcc ctcttcttag gacgcggtga
    64921 cataaaaaat ttgccggtct tcggctgtga atttccaccc cagccgcttc tcaaatacca
    64981 cccgatgagt gccggcaacc gagggcttga actcgaaact acgctgccct tgatcgaccg
    65041 cattgttgct ggcggtacga agaaaatcat cctccaccaa gggcatgact gcggcgtcat
    65101 agatcggaac ccataactct ccccgcgtgc gatcttccca aagccggata tgaaccggca
    65161 gtcccactcg cgccttcacg actttggcac ccggctcctg ctcggtacct gtcggcaccg
    65221 ctttgctcag tggactcatc ttcctaacaa ctccaatatc atcattccgt gcggctacac
    65281 cttgcgccgc cctccgacga atatctgccc gtcttctacg cgcacatcat aacttccgac
    65341 gcaatacccc ccgccgtccg tgccttgccc atttcgcaca tcaaatgcca agtcgtgcca
    65401 gggacaggag acgacatgcc ccttcacgcg ccctttattt aaaggaccac cttcatgagg
    65461 gcacgaatta tagatggcgt gaaaatgccc ctcaatatta aagaccgcaa tggcacgctc
    65521 attcaccttg acgaccttga cttggccggg aggaatatct tcaaccttgg ccacgcactg
    65581 aaacccttcc ataaacgatt ccggctcccc ttactgttac atccgtaatg tgaacttgag
    65641 ctccataccg atggtaattc ctgcgcgctt tgcgtacccc gcctctgctt cgattaagta
    65701 gcgaaccggc tcgggaggcg gaccgtagaa cgggcaagga tcctggaggc agggctggac
    65761 ctgctcgaga atgtgtacca catgatgact ctcatcaatc cacaacatat caacaggaat
    65821 gcggaattga cgagtcgcga cacggtgcaa accggtcgat tcaaagatat aaagcatccc
    65881 cgtatcggct ggcaatcctt cccggaaggc caacccgaac aggagctttt ccggggtgct
    65941 ggccacctct gtctccatcg tcttcccgtt cgggaattcg acaatgatga gttcggattc
    66001 ctttggaccg ccgagaaaca ggctcacact gatcagcaag atcactaaga gcacgaacgt
    66061 cacaattttc ttgcgacgct gttccgattc agacccgctc gcgcgcgtca ccataaccaa
    66121 atgctgtcgg acatggatgc atcctcatgt agtcgtacct gcgtagagcc gcatgtacat
    66181 tgggcccaaa gccccatgac tttcctggtg ctaggccttc ttcccaagcc tcattctgtt
    66241 gacttgccga agaacgccag catagaatgc ggacctgttt cgccgcgaac tatggtcgcg
    66301 gcggcacgaa attgtcaaca tcttatccgt cattaaagga actgcgaagg aggcataggc
    66361 catggcgctt ctgatcaccg atgagtgtat ttcctgcggg gcatgcctgc ccgaatgtcc
    66421 caacgaagca attttcgaaa cgcgcagcga tgctgaaggc aagggcaatc acgtcggaga
    66481 cgggcaggga gtcggtgata acatctacgt catcacccat gatcgttgca cggagtgcgt
    66541 cgggcacttt gacgagcctc aatgtgcggc tgtctgcccg gtggacaact gctgcattgc
    66601 tgacccagcc tatccggaga cgactgacgt attgttggaa aaggccaagt cattgaatcc
    66661 ggacaaagaa attgatccgg ctaaggtctg gagcggcgtt cgtaactaat taggttcagt
    66721 acatgtgcgt ggcaccacga aggctcctcc gggagccttc gctgtttttc cactaaccag
    66781 cggtgcaaca acagatccat acctggcttt ccgcagtcga tcgctgtaga ctttaagtaa
    66841 attgtggcat gcgcaggcca acttgcccct acgtggcccc gcggcgtcca ttcattggcc
    66901 ccctatccac gggaggtaca catgaactgc ttcacgcgcc ttgcatgtat tgccatcctc
    66961 gctctcgcac cgctgccttc ctacgcaatg acggaggtag gggatatcgg cacgatcatc
    67021 gaaagtttca tcacaaagca atttcctgat gcggccagtc atttttgggt ggtgaacgac
    67081 acccaatggg acggcgacga aatgatcgtg gacatgaata ccgtggtgac agaccggcga
    67141 gaggcgccgc cgacggagag tcggtttctg ctgttgatcg tggaaggacg cctgcaaggc
    67201 attcaaaaca ttccgctgga tgctgccgca gaatgtcaaa aggaacagga agtctaggca
    67261 agaacgattt cgatacaggc ccttgatacg acaacgcccc atctgcatca ctgcagatgg
    67321 ggcgttgcaa ctcgtaccgt tcaaactcag attacttaat catcaccagc ttgccaccga
    67381 cgtgagccgg gtgcagatgg cagcgatagg tcagcgtgtt gccctgcgtg gcattgaaca
    67441 tgtcgctggt aggcacaccg atatacttgg tctcgccagg cttcagcacc acttccgctt
    67501 tcatgacaaa cggtgatgct gcgttgaccg gatcggtcat ctggaagcca tgctcgctgg
    67561 aggagttatt cgtcactttg agaacaaccg gacggccggg gcgaacttta aaatcaatca
    67621 ccgtgaccgg tggataccag gccttcaagt tcccgatctc gacggcatac aattccgcat
    67681 ccacaatcaa ttctttaaat ggctgaccga ccacggagcc ggtatccaga tcgccgatct
    67741 cgacaccgcc agcttgcagc gcatacgcgc cggatgcccc agccattaac atgaccaggc
    67801 caaagaatac agctaccatc gtcttgccca tgacctacct ccttaaagag attaagaaga
    67861 gatgcgatta ggttggtgaa taatgaccac cgcgatctga accttctgct agacaccgcg
    67921 ccccggaggc tagcacacac tgctcggaaa tgtcctaaat aaaaggctga tgagggtcac
    67981 cttatagcat agggatttaa aaggaagcaa gaaagttctt ggacggtcaa gacggggtct
    68041 ttaggcagga aatgctcgtc gcatgtagag gatagatcgg cacttcgtag ctacatttca
    68101 accacttacg gcgatgaatt cccctctggc gacatttcga ctgcatggga gccgatcagc
    68161 aggtcatacg gtgtgtagtc gtccgtgagt gtcagcccgg gcggccaagg cgtggcacgt
    68221 cgcgaggcca gcaaggctat cgcttcgagg ggcaatcgac gggcttccac ctgcatgtaa
    68281 atacgcttca cgatctcgtc atacgacatc gtagcgatcg ggccgccacc gaagaaaatg
    68341 agattttctg ccgtggtctg gtgcgcacgc cacggtccct ttaccccaaa tgattcaatt
    68401 gagggaaagg agcttttcaa tgtctgcacc acggcattcg cacgttgaag atcgccaccc
    68461 tcccctgaag aagccagatt gagagccaac acgccattgg ggttgagacg tcctcgaagc
    68521 tccgaaaaga attcgaccgt cgtcaaatgg aagggaatga gatgccgggc aaacgcgtca
    68581 acccagatga cgtcataggt cgctttcgtg tcgcgcagaa acacgcgcgc atctttcact
    68641 gagacatggt gattggcagg agggcgataa tcgaaatact gttccgccat ccgcaccacc
    68701 accggatcga attcgacaac atccaactct agctcaggcc agacttgagc aagccacttg
    68761 gccaacgagc cgccgccatg tcctaaaatg agtcctcgtt tcggctctgg agccaatgcg
    68821 accgccgcca ccatcagttg gctatagggc aaaaacaggg cggtcgggtc tgatcgccac
    68881 atgaccgcat gccacgtgcg atcgagcacg agataacgaa acagatcatc gtcacggaca
    68941 cggacttgct gataggggct atcttcctga tagaccaatc ctgcgaccgg aggagcctgg
    69001 ccgaacagtt gccaacccga tatgagcagg ccggtcatca ccaccccggt taacttcgtg
    69061 actctcccgc ttccttgttg caaccagagt aatcccaaga gcaattggat tacccccagc
    69121 gtcgctacga gggtctgcgt gcccaaccac gaaaggagaa agaacgccgt cccccacgtt
    69181 cccgccagac tgccgacggt agataacgca atcatgctgc cggtatgtcg ccccagatat
    69241 cccatatcga caacggctaa tcggagcaat gcgggcatca caccgctcag gccgaatgcc
    69301 ggaggggcca gcaagacaca ggcggcaagg ctggggcccc atcgcggatc ctcgacgagt
    69361 ttggcaacct ggaaaatggc gccctgcccc atccaggaca tcagcaacgt ccacgcgccg
    69421 gatccgagca gcagccacgc caacaccgcc gcaacacgat accggtcgga agcccaaccg
    69481 ccgaacgcat agccggagct catggccgcg agaatgatcc cgatcaaggc gccccacaca
    69541 aagagggaat ttccgaagac aggagccagc agccggctgc ccagaatttc gagcgccatg
    69601 acgatcgcgc cggtcacgag cgcggtgaac agcaagaaga gacggggaat gggaacaacg
    69661 gcaggtcttt gtgccatcat cgaccaaaga ccgaaagaca ccgtgagggg aagcgacaac
    69721 agcgggcggg cgagcacacc atgctacaaa gtaccccaat tcttgaagta tcgaagcaga
    69781 tcgcgaacag agatcatccc caccacacgc ccttcatcgg taatcaccag atgccggatt
    69841 cctcgttcag ccatcacatc gctcgcctca tgcgcggatt gggcaatatc gatcgtcatg
    69901 aggggggcac tcatgacgga gcgaaccgtg acctggtctg acgcggcccc gctggccatc
    69961 gccttgcgaa cgagatcggc ttcactgaca atgcccacat agtcgccatg gtcagcgacg
    70021 agcatggccc cgacccgggc atcgcgtaat tgctgcgcta ccaccaataa cgaatcatcg
    70081 ggtcgcacgg tcttcgtaat gggacgcatc atcatggcca acggtcgttg gatgggcccg
    70141 cctgcaacca ccatgcgcat ccctcctgcg aagaggccga attctactga agtacgccac
    70201 aatctgcaat tcatcctgcc atgcgctatc cggcacggac tgcaaaaacc tccgagatga
    70261 ttgctcggtc tggcatgcgt tgctatacta cgcactctac acgaaacgct cacccacgat
    70321 tcgcagcttt tctttaagag gagatgcatc catgcatatc agcgtgattg gaacgggcta
    70381 tgtcggcttg gtcaccgggg cctgctttgc agaattcggc gtgaacgtca cctgtatgga
    70441 tacggacgcg cgccggattg ccaaactgga aaagggtgaa gtgccatttt tcgagcccgg
    70501 catcacggaa ctcgtcgcca aaggcatcaa agaagaccgg ctccatttca cgactgatgt
    70561 cgccaaagcc gtcgataaag cccttgtgat cttcattgct gtcggaaccc ctcccaaatc
    70621 tgatgggtca gccgacttgt cgtacgtgga agaggtggga cgcgggattg cgaagaacat
    70681 gaccgggtat aaggtcatcg tcaccaagtc caccgtgccg gtcggtaccg gcgaaaagct
    70741 gcgcgaagtg attaaggcca accaaaccgg tcgattccgg tttgatatcg tctccaatcc
    70801 ggagtttctg cgggaaggct ccgccattga agactttatg cggccgaacc gcgtggtcat
    70861 cggcgcagac agcgagcagg ccgtcgcgat catgaaggac ctctaccgcc ccctctatct
    70921 gctcgagacg cccatcgtgg tcacggacat tccaactgct gagatgatca agtatgcctc
    70981 caacgcattt ctggccgtca aaatttcgtt tatcaacgag atcgcgaccg tgtgcgaaaa
    71041 ggtgggagcg gacgtccagc tggtctccaa gggcatgggg ctcgacaacc ggatcggcaa
    71101 caaatttctg catgccgggc ccggcttcgg cggatcctgc tttcccaagg acctcgcggc
    71161 gctggtccag accggtgagc gtgtagggta tccgttccag attgcaggcg cggcagcgaa
    71221 agtgaactac gaacagcacc tgcgaatggt tcagaaagtg aaggatgcct gcggaggagt
    71281 aaaggggaaa accctcggcg tgttggggct ttcgttcaaa cccaacacga acgatatgcg
    71341 cgaagcgcca tcgttaacca tcctgagcga gctgatgaag gaaggggcaa agatccgcgc
    71401 gtacgatccc gcgtcgatgg aagaatcgat gaaactgctc cccggcatgg tcccttgcca
    71461 ggacacctac gaagtcgcgg aaggggccga cggcctcatt atcatgactg agtggaacca
    71521 attccgaaat ctcgatttcg aacggctcaa gaaagccatg cgggaaccac tgttgctgga
    71581 cctgcgcaac acctacgagt cggaccgggt cgtccccttc ggcttccgcc acgtatcagt
    71641 cggtcgtcca accaagaatc cggccgccta accggcggcc ggacgggtct cgctaactga
    71701 gcaacttcgc cggttcaggc tgagcccctt ctttcgcctt gggcttataa cccatccggt
    71761 ccaatacctt catgatcttg gtcttcaacc ggtcgtcagc ctccgccagc gccgtggcga
    71821 ggggttcgac ggccggcttg ccgatcttcc tgatgatttc cgtcgccgac tgacgcacat
    71881 cgtcttcttc ggaggccagc aacgggacaa gcgagggaac ggccgacccg ccgatcttaa
    71941 tcaacgaatc ataggcccgc tgccgcacat cgccaacctc atcggtgagc gcctcaacca
    72001 gaggcaccac cgcccgcgga tctttcaaat cacccaggac ttccgctgca tatttacgat
    72061 tgagccagtg cgaatctttc aagtcgatca gcatcgcgtc ggctttggcg gcattcggat
    72121 ctttcgcgcg aatccggaag ctcttcgcca ctcgcttgcc gccttcttcg acaacacgaa
    72181 tcgtggcgcc gtcacccggc ttgatcttga ggaaatcctc aagggcttct tctccgacat
    72241 caagcacgac ggtctttccg tcataggcca acagctcaac ttccagctgc ctcgcttccg
    72301 gattgacggc cacgacgcgc tccgtcacca ggttgaagcc gtccttcttg ggtccgccct
    72361 tcgggccgat ctgaatgagt tttaccggtg cttcttctgc catagtagag ttcctttatc
    72421 gggttatgac gaggtcgcct gtttccatcc caaactcgcg agtacacctt cgacggtctc
    72481 ctgcaccatc gtattttcat cttccaggag aggaagcagc ggctcgatgg catgcttcgc
    72541 cccgaggcgc gccaaggatt cagccgcgtt ccggcggacg agccagtcct catcctgcag
    72601 cgattgaatc aacgcgtcgg cgctgcgcgg atcaccgatt tttccgagcg cttccgccgc
    72661 atggcgtcga accatccaat tggggcccaa gagacccccg atcaacgcat caaccgctcg
    72721 agtatcgccg attttcttca acacccgagc gacatcctcg cgcaccgtgc catcgcccag
    72781 ggcttcaatc aacccgggcg tggcccgcga gtcgccgatc cgctccaacg cccagaccgc
    72841 cgcagtcctg acagccccgt ctttgtcttt gagcgccacc atgaggggat caatggcgcg
    72901 gggatcgcga aaccggccga gtacatttgc ggcctgctcg cggatcgccc attcatcgtc
    72961 tcgtaaggct tccagcagct gatcgaacgc aatgggccct atacgcacca ccgccgtcgc
    73021 ggccgcctcg cgaatgacca catcctcgtc ggccatcagg ccgatcaatt ggggaacggc
    73081 ttcaattccc atctgtccga ggctcgccat cgcatggtcg cgcaacgcct cattatcgtc
    73141 gcgcagcgcc gctatcaatt gtgcaattcg ctcggcatca ctcatgatcg tccgattcct
    73201 cctgcccgac cggctgttcg ccctcgagtg ccgcaagttt atccgcaatg aggccggcgc
    73261 tgtacgatac gataccgtcc cgatcgcttc gcaaccggtt gaacagctcg ttatacggac
    73321 gcaagacctc gacatctttg attttggcca gcgcctcgat ggccatcacg cgcaagggcc
    73381 ggatcgggat cgcttcgata tacaattggg ccggccgcgg gtcgccaatc agccccaacg
    73441 acttcacggc atactccttc acaccggtat ccttatcctt ctggagccgt ttcatcaacg
    73501 gttcgaccgc acgcacatcg ccgattttgc cgagcaaatc ggcagcagct tcacggatga
    73561 gccaatcgtc gtcctcaaga tattcgatca gaatctcaac cgacggccgg ccgatcccca
    73621 gaagatccat caccgtcgcc atgcgggctt cctcgcgttc gctcgcgcct tcgacgtcac
    73681 gcaacgcatt aaacgtcgca tcgatccgtt cccggaccgc gctcagcttc ttcaacgtcc
    73741 gcacgacgat ttcgcggacc gccggcgaag ccatggcatc gatcaatccg tcggccgaac
    73801 gcggatcgag cagatgctcg agaatcgtcg ccgccgtcat ctgagcctcc gcatccggat
    73861 ggcgcaacat ctcacacaac ggcgtgatcg cattgggaat cacgcccaag agctgccgaa
    73921 cacggtgacg gacttcctca tccggtgact cgtgaaggtt ccgcactaag cccgccgcag
    73981 tctcgtcgtc cagattaccg gccatctgtt caagcgcggc aaatgccgcc tgccgtaccg
    74041 tcgcttcagg atcccgaatc aaggtgacca gcgcaacacc cgcagcggga tctttgatgc
    74101 gggacaacat cgcggccgct tcaacacgaa gagggacgtc gcccgactgc aacgcgtgcg
    74161 tcaaggcctg gacggcccgt ggaccaccgg ctaggcacgc cgcggtggct cgcatacgcc
    74221 gccactcctc ctcatgcgtc aactcggaaa ctaaggtatc gatggactct ttggacatcg
    74281 tgaaaaagtt gcgtagccga tgttttactg attgcttacg atgaagcctg cttccgaaat
    74341 agcgttctca cacgatccgc acagcgttac gttcagactc gcccggcgcg ccacccgacc
    74401 gccgtaagtg tttcccgaac gtgataccgg acattctcat cctgctcttt ggccaacatc
    74461 ggtaagagag acggaatcac cgccggaccg aatttcgtca gggccgccgc ggcttccgac
    74521 ctggtgatgg tattccgcaa ggctacgatg agtgatggaa tcgccacggc atcggcaatc
    74581 atccctaatg cacgggccgc cattcccatg gtcgccatct catccgtcca tccgtcggca
    74641 cagggtgtgg ccgaatccct cggctcaccc agcgggacgc cctcgacaac ccgccgcaaa
    74701 atcggcacga cccggcggtc gccgatatgg ccaagggcct cgacggccaa tagccgtaat
    74761 cccggctcct tcatcaccgt taccaaataa tctaccgcac gcccgtctcg tatcgcaccc
    74821 agcgcccgga ccacatcttc acgaatcgct gagtcccttt cattgaataa ggccctcagc
    74881 aagggctcga cggagtccgg cgacttgagc tttcccaacg cctccaccgc atgaaggcga
    74941 accagccaat catcatgctg cagggcttcg atcaatcccg ccatcgcggc cacgccgatg
    75001 gccgccagcg cagtggatgc ttcctcgcga acagccttga ccttgtcctg cagcagcggc
    75061 atcaacgcgg acactgcttc cggatcgccg atccgcccca ggccctttgc ggcgtgcatg
    75121 cgcacgatcc aatcacgact gcccaaggcc tgcatgagag gcgccaggac gcgcgcatcg
    75181 gccagtgaag ccagaatggc cgaggccgct tcttgcacct gcaaggccgg atcagccaaa
    75241 caacctgcca aggccggaac agaaacggat ccgagtgatg agagggcacc cactgccgct
    75301 tcgcggacag cacgatcggt atcgcgcagc gcatgagtca aaggcagtac ggcccgcgca
    75361 tcgtttaagc tccccagcag ggtcgccgct tcctcgcgaa cggcccaatc ctcatccgac
    75421 agggctgcga tctgttcact gacggtgtcg gccatggtac ctcggcctct gggaggccat
    75481 cgaccttaac acagcatttt tttaggggtc aacgcgggtt ggccctacga cctgacatcg
    75541 caccatcccg cacgctggtc gaaacggaga ggaagagacg cggggacgcc ggaagctctc
    75601 aacaggccag gcaccccgcg agagaacgcc gagaattacc gcaccgatcc tttcgcaaaa
    75661 tctccgctcg gaccgaagcg accgatatct cccaacggcc gatcgacacg aagattgtcg
    75721 gcctggctgg aatccaccga ggtttcttca tccggaggtg tccatcccag gtgctccagc
    75781 gtttccacga ccagcaaccg caaggaatcc ttcgcggtca accgcaccga cgcgtaagac
    75841 agcacccgct cgccttcggc ttcaacttcg gaggccttcg gatcgtacag gaatgaaatc
    75901 agcggctcga tggccgaatc gccgatcaac accaacgctg cggcagcctt ttcgcgcaac
    75961 acgccatcct tcaacagcat gatgagatcg gggatcacac ggggatcgcg gaaatgtcca
    76021 agtgcggtcg cagctgcttc cttaataaac gcgtcttcac tgacgataca ccccggggtg
    76081 ggcgtcccat cctgacgaac acctttgccc ttgagcgccg ccagcaccgc cggaagagcc
    76141 cgcgggtcac cgatcatgcc gagcgacgcg atggcatgac gcttcacggc gccatctttc
    76201 atggcttcga gcaacccatc gatcgcgcgg gggtcaccga tcatgcccaa ggcaatcgtg
    76261 gcatcttcgc gcacggcccg gtccggatct ttcagggctt cgatcagcgc atcaacaacg
    76321 cgcggttcac gcacccaact ccggccgatt tgatagtccg tcgtcatccc acccaatgcc
    76381 cgggctgcat ggcaacgcac cacaaaatcc ttgtccttca tcgtttcaag cagcaggtcg
    76441 acggatggct gtccaattgc gaccagcgcg atgccggcgg tctcacgcac aattttcgag
    76501 gaatcccgga acaacttgat caaggcagga atggctttgg cgtcgccaat ttttcccaac
    76561 gcttccgccg ccgcgctttt cacagcaccg tcgcgatccc gacacgcaaa gatcaaggcg
    76621 tcaaccgcgc gtgcatcgcg aagatcgcca caggctttcg ccgcatgttc cctgacacgc
    76681 caccgttcgt ccttcatcgc cgcaagaagc cgatcgaggt gccccacgcc cagggacacc
    76741 acggccgcga ccgcttcgtt gcgaacttcc atgatgggat cgttgaagag tgcaatcagc
    76801 gcttcgatcg ccttcgggcc gccgattttg gcgacacccc agccggcgcg cgtacgcaca
    76861 gaccaatact cgtcgccgca ggcgccgatc agcgcgtcca gcgtggtcgg gttcgccaac
    76921 tgcgccaagg ccttggccgc ttcttcacga gttgcatcat ccggatcttc gagggaatcc
    76981 agtagatcat cgagtgcctg tgccatcacc cacctcacta ttcccataat aggcatcacg
    77041 ttgaccgcca tgaggatagg tgcgcttgca ccgtaccacc agggtctgag tcccgcggcc
    77101 ctccacacat tgatcgagtg acttggagta ttccagtttc ccgtccaaac tcaccgtgaa
    77161 gggaaagtcg aaggtaaaac tgatgaactt atattgtccc ggcttgaggt gcaattcgcg
    77221 agtctcggtc gtccggtacg catccgacgt ttctggatcc aatacccaga gcgggggtgt
    77281 gatgccgggc acctgccacc aggattgcgg caccaacgga gtcggatcga ttgtaatcgt
    77341 atgttccttg gtataggcag gcggcggacc ggctgccagg agccgctccg catgtccagc
    77401 gaacacagcg catgacaatg cgacgatccg gatcaacgtc tgccgccccg taaacaatct
    77461 gtgcgacaag atatccattg gtcctctatt gaaggagtgg attgacagga gcaaccgtga
    77521 acgcggtctt aaaaatttcc tcgaccgggt gactctccca ttcggtgtag gtcgtcagtc
    77581 caagcgcgcg catgacagtc gcgcccgtat cgataatcga cactggctga cgaatcgcgt
    77641 aaccggcctt gatgccgacg cctgaggcaa tccagggcac gaccgggacg gcgcctgctt
    77701 gatcggcgga catgtcaccg gatgaaaatt gcgtctctcc aaaggcgctc agcgacgtcg
    77761 caaagaccgt cgtccgtttc atcaagccga gttcacggta gagatccaac accgtgccca
    77821 tcgccttgtc gacagctttc aatgcgtctt tgtatgcggc tgatttccag ccctgcgctt
    77881 cgccgacacg accgggagcc ggcagatgca cgaccagcag gtgcggcaag gacggaatgg
    77941 catggccata tccggaaccg cttgtggcct tcttgaagta gtcacgcaca taccccacca
    78001 accggtcggg attgcactcg gctctgagtg gcccgcacat ctgatagtcc gtatagggtt
    78061 ccggtttggc gagctgatac agagattcgt ccatgtagaa aatggcactg tcccggccgc
    78121 cgctcaaatc gagataatca aacaccgtcg gagcgcgcgg gtatccccgg ctgaattcaa
    78181 agacgttcca ggtgatgcca tgtttggcga ccggcatccc cgtcaccaag gatgccatgg
    78241 ttggtaaccg gcgcgcaggc gcaaccgccg tcgccgacca ggtaacggcc ccctccttca
    78301 cgagagaaga caagaccggc atcacaccgc tcttcaatga ttcctggcca aacccttcca
    78361 ggacaaacag gatgacatgt tctgtcatcc ctgcggggac cgaaggcgga atgtctcctg
    78421 caccccgtgc agccaaggcc accgaggaag aggacgaggc cacgacggtc atactcagaa
    78481 caaccgccag gcaaaacgca accgggacgg tggaaatatt gcggataaaa ctcatgagat
    78541 tgcctcacca gacgatcgat ttatgaagcg aagaaaaagt accttagcac gctgttttcc
    78601 gggaaggcaa ggcacgaacc tcggctctca tagcgcctga gcgggttcga tggtatcctg
    78661 gtcgaatcgg gaagaggcca gtgcgatcaa ccctccgaac acccatttta cagcggtgca
    78721 ccacaatgcc cggcaaccgt tcttccagtg atttcttctg cctgaccgtc gagtgttcag
    78781 ggaagcgtcg cctcatgccg ctcgtcgctc ttttcacagc aggctttctc ctcttcttcg
    78841 cctctcctag cccaagtctt gccgacgttc gcgtggtgac ggccacagga cagcatcgca
    78901 tgacagactg ggaaaccagg gtcgatgcga tccggctcgc cgcggagcag gcgaaacaac
    78961 aggcgctgga acaggtcgcg tcatatctgg aaagtgtcac cgtggtgcag aatctcgacg
    79021 tgactcaaga tgaacttcgc agctacacag ccgggatggt cctggtgctg gatcaacagg
    79081 tggacaccgc tcttgatgga gatgtcgtgg tgatccgcgt caccctcaaa gcccaggtag
    79141 acagcgaaga ggcggcgcag gccattctcg cgatgaagca gaacgaggac gtgcggcagg
    79201 aattactcgc cctcaaggaa gatgtcgaga gcctgcataa acaactcgaa gacgccaatc
    79261 aggcgctcgc ccacgcgaat tcacctgagc aggtcagaaa actcaaccag cagcgtacgg
    79321 atctgctcaa tcacgccgaa tcggatgcca tggtggcgca ggcctggacc gactggctgt
    79381 tgattgcccc cttcgcgcac ccctaccctt cagcaggcgt ggctcaggtc caagcgctgc
    79441 ttggcatcgc agggcggttg aacccgacta atcctcacct cgccattgca caacagaccg
    79501 taggagccaa gctccccccg gcaccccctc accctcgaca gtcacccgtg ccgcataccg
    79561 tgccattttt gccggggtac tatgtcgtgc cgaaggaacc ggctccccca ggcacacctc
    79621 cacccactga ttccaagcag ctctcaagcg tctaccaact caatccgttg ttgcccaatc
    79681 catccgggca accgccacaa tccggcaaca gtacgacgat tatccaggct catccccgac
    79741 ccggcacctc accgtccgtc ccggaccgca acatgatgcc gacctatcga cagatcccgg
    79801 cgccgaatag tcaggcaagt catccgactt tgcataccta tcgcgccgga ggcagtcccc
    79861 cctcggcgtc gataccagga tcggctgacg gtggcattcg aggagggcgc cccccggtca
    79921 tccaccaaac gaaaccgctt tcgccttccg agtccactgc ggaacgcggt tccgccacca
    79981 atcaagggga caaatagcca tgccactgac atccagtcac atcctcatgg cagtagggat
    80041 ggccgggact ctgcttgtgg ccacagccac catggcaggg gaactcacac cccaagacgt
    80101 tcggacgcat gccgaccaac actttcagca gcttcgcgca cgagaacaga ctattgcacc
    80161 aacaaccaga gaccggccgc agcaggaccc ttcgtcctcc taccatcccg accaattttt
    80221 catcggacag ggccagggcg atttgagtaa aggaaaactg gtgtgtcagc gagtatccga
    80281 attggccgct cgggctgaca tcgccaaaca aattcgggtc caggtcaagg agcatgccac
    80341 cgaccgcctg agggaacgca ccggtcgtga accagagcag gatatcgaag tggttcgcga
    80401 agaaatcgtt caagaatatc tgcagggcgt gacgatcgtc gatcgccgca tcgacgacga
    80461 agcaaagacg tgtacggcgg tggccgttat gccgaaagcc accctcaata catcacttcc
    80521 ggccgaatcg tccgcccgtc actgagcgtc cccaccttcc gaacatcccc ctccattgaa
    80581 cttcggactg cgtttcgggt aagacaatct tcccatgccg atcgaaaaca acttccagca
    80641 tgacgaactc tccaggaaaa atcctggcga tcgtctcacc tatgcggact ggacgccgcc
    80701 gcctgacatg gattcgcccg cctggcaagc cgctatgggc gaggccggcc agaagctgtc
    80761 gcaggccgga gtgcgagtaa tcgtctttct gcatggggct attcccggca ccgacatctt
    80821 cggtttagga cgactcgacg aagtgggcgg cctcaaacgc ggatattcac gcggcatctc
    80881 cggattggat tcgctcctgt cgtttatgcg agaaggcacc aacggcatca caccgctgcc
    80941 aggcggcatg aaaccgccgc tcgccaatga cgaggcgacc aaaagcctgc tcgatagcca
    81001 gattggcgac gcaggcaatt tcactgcacc gatcgtggaa cggtgccgca aggccctcaa
    81061 tcagcacgtg tccaaaccca tcgtctgcgt acgcgaactc tggtccagcg agcatcacca
    81121 tctcggccgt gccctggcgg cctgccggct cttggatcgt ctgcgggcct tagtgaccga
    81181 acaccatctg ggaaccgggg atcgcattct gattcaagcc catgggcaat ccggacttgt
    81241 gctcgcgctc gccgcgaatc tactctgccc ttcgcccatt accggccgga aagcgttttt
    81301 cgatctgctc ctggcggcga acccgcagca ttcggatgtg caggccattc agcgcatcga
    81361 tcctttgttg acttcgggct ccctcctcaa cggcgcggcc ctcgacatcg tcaccttcgg
    81421 cacaccagtg cggtacggat gggacccctc cggcatcggc aagcttctac atatcgtcaa
    81481 tcaccgcaat ctgcgcaccg atggaaagac ctggctctcc aaaatggagc tcccgcagat
    81541 ctccgtcgaa atgccgattg cgtggggcgg cgactacgtg caggaattgg ctgtggccgg
    81601 cagcgatgcc ctacccgctg agagtgccaa agcagccaat aaagccgtgt gggaattgct
    81661 cgaaccttac gatgggtttg aacgttgggt ggaatgcgcg cgccgcgcta cccgctttcc
    81721 cagcgaagga cagtgcctgc tcgtcgatta caaagactgc acgagctcaa ccaacgtccg
    81781 cgaccattac tacggccacg ccgcctacac gcgcgcaaac gcgattctct tcaacttcaa
    81841 tcagatcgtc gggcggctgt actcgtaagc gtgacctaac acatcccgct acgaagtcag
    81901 ctttcgttct tccgtggcct cctgggcgcc cctctcaatt tccttcttga tttgatccca
    81961 tgtcgccgtc tcgggcaatc gccggagctg cttaatcgcc gttttctttg gcgtcagtac
    82021 ctctgcttct atgctcaggc cagctcctgg tgttcgccga acagcatctg caaaggcctc
    82081 ccatttatga tcatctgaaa ggatctgctt cagaggagaa ctgtgttcta atgaccaagt
    82141 aagaccgtga atctcataat tggcgtatct atcgaccctg ttaggcagga gaacctctcc
    82201 cttcttaaca tctacataaa atgcgacgtt ctttatgaat ggggttgtca tgatctcggt
    82261 gggacgagca tgcattctcg gatcgtttaa atacttttga tcctgctttg cagcggaatc
    82321 ggcagaattc ttagtgggaa cgacactgcc ccatctgctt ttcgaaagcg ttctaaaagt
    82381 ctccaaaaga attgtttctg gctctatggg agaaccggcg cccgattcgt aaggaactac
    82441 gttgagccaa tcttgaacct gaatttgtat gccttcaaac gtctggtaaa tccattgctt
    82501 cacattatgg gcattaagac ggttccctag atcactgctc ttctcaggaa agattgccgc
    82561 gaccgtacag gccaccgcct tagcaaattc ttccaaacct ataaccgcta gggctgccgc
    82621 ccttgaatta tgttcgtttt cggccagaag tctcgcttcg gcacacaatt gattggcatt
    82681 cgtcagcgcc gcgactgcag ccaatcggaa ggtcttagca gctgtcattt cgtgaatatg
    82741 tctcccgaga catgcggctg tggacatcgc agagagtcgt gacagagaac gccgctggca
    82801 gcctgtttca gtatccggct aatatggcat ctcgtcgtcg tcaaacccga aatgatgacc
    82861 gatctcgtgc acgaccgtat cacgcacttc ctgcaccact tcctgtttcg tgcgacacag
    82921 tctgaggatc ggtccgcgat agatggagat tttggccggc agttgcccgc ccgcctgaaa
    82981 gaaagactct ttatcgatgg gaaggccttg atagaggccg agcagatcct cgtcctcgtc
    83041 catgtcaaga tcggcgagga cctcggggga aggctcatcc tctaccacca cggcgacgtt
    83101 ggtaatgcgc ttggcgtatt cgtcaggaag gccgtccagc gcctgctgaa ccaaggcctg
    83161 aaactccggc tctgggatgg tcaatctacg ctttcgcggc gacatgggct gaactgaacc
    83221 aacttacgct ctacacaaca gctgctttgt tcaggatgtt caaacaggct gtccggcaag
    83281 gccgcatgga gtgcggcgac tgagtcatac agcctgggag atgtcgcagg aagccgtgcg
    83341 actgagaagg ccgctggcaa cctgtttcaa catccggcta cggatgggcg gcgcgccagt
    83401 tacacccaac tccgacatcc actttgagcg gcaccgataa ccccaacgcg tcaccggtcg
    83461 cttccatctc cgctttgacg agttgcttcg cctgctccag ctcctgatcc ggcacttcga
    83521 agatcagttc gtcatgaacc tggaggatca ttttgcagcg cggcagttcg tctcgcaagc
    83581 gccgatgcac cttgatcatg gccagcttaa tcagatccgc cgccgagcct tgaatcggac
    83641 tattgaccgc catgcgctcg cccaccccgc gttgagatgg atcaccgctc tgcaattcgg
    83701 gaatgggacg acgacggccc agaatggtgg tcgtgtagcc cttggttttc ccatcgttga
    83761 tgttgtggtc catcagagca cgcaccgctg caaacttttc aaagaaggtt tcgatgtatt
    83821 ttttggcctc agcttgtggc acaccgatgt tcgatgcgag gccgaacgga ctgatgccat
    83881 agacgatccc gaacacgacg cttttcgccg cgcgccgcat ttcgcgcgtg acgtcgccgg
    83941 caggcaggtt gaaaatttcc atggctgtcg ccatgtgaat gtcttccccc ttctcgaaca
    84001 cttccaataa tcgaggatcc tgggagaggt gggccagaat gcgcggctcg acctgactat
    84061 agtcggcaca gagcaggtga tggccgggcg gcgcaataaa ggcctcacgg atccgcaggc
    84121 cgtagtcgcc tttcaccgga atgttttgca gattgggatc ggtcgacgag agtcgtcccg
    84181 tagcggcgac cgtctgattg agcgacgtat gaagccgctt cgtatcagga ttgaccagct
    84241 gcggcaatgc atcgacatag gtcgatttga gtttggtgag cgtgcgatag ctgagaattt
    84301 gcgcgggaag ctcgtgctga ttcgcgagtt gcgtgagcgt gtcttcatcg gtagaaaacc
    84361 ccgtcttcgt tttccgtagc ggcttcaggc ccagcgtttc gaatagcacc gtggccaact
    84421 gcttcggcga gccgatattg aattcgccgc cgcccaaccg ataaatggtc ccgaccatct
    84481 gctcgagttc ccgctccaac tccttgctca aggtgcgcag cccctcgaca tcgagcaaaa
    84541 acccgttgcg ctcgatgtcg gctaacaccg gcaccagtgg catttccact tgttcaaaaa
    84601 gcgacagact gccctgctct ttcagccgat cgcgcaacat gggcgcaact ttggccgtga
    84661 ccgccgccgc ctcgccggtt cgacgaacca cacgttcatc gacatcaaac agagactgcg
    84721 gttccttgcc cccttcctcg cgttcgcccg tgccgagctg ataactcagt aggtccatgg
    84781 cgatcgcttc caaggtatgc gcccgtcgat tgggattgag caaataatct gcaaccatcg
    84841 tgtcaaagta gggacctggc atgtcgatcc cctgccgctg gagcgctaac agagtcggtt
    84901 tgagatcctg caccgccttc ggcctgctcc cgtcccgcac atacgcggtg aggggctgcg
    84961 gccagtcttg cgcctcccct tgtacgaacg cgacagtccc gtccggccag ccgaaggcac
    85021 agccttgaat gtcggctcgc acgccggctt catcactcaa tacacaagcc agccccatca
    85081 tggtgtccgg ttgtcgccgt ttcaaaaaat cttccgctcc ggcagtatcg tgtatgcgct
    85141 gaacctcagc ccctaaccgg ttctgctcag gagtgtcgcc ctgaaaagcc ttggccaacg
    85201 tcatgaattc cagttcgcgc agcaggctca ccagcacttc agtctgcggc gctctcgcct
    85261 gaaaatgagc gggggcaaat tcgatgggac aatccacctg gatggtcgcc agctgcctgc
    85321 tcatgcgggc gctctcaccc tgttccagca acagattctt cgttttggcc ggagtcactt
    85381 cttcgacgcg gcgcaacagc tcctcgatcg tgccgaattg ggtgatcaat ttcacggcgg
    85441 ttttctcgcc gatccctttg acaccgggaa tattgtcgct ggtatccccc atgagaccca
    85501 taatttcgac gacacgcgcc ggctccacgc cgaaccgcac ctgtgaatcc gcctcgccga
    85561 accacttgtc tttcacggga tcgtaaatgc gcgttttggg agtcagcagc tgaaacatat
    85621 ccttgtcgct ggtgacgatc acgacatcga ggccttcgcc ttcccccttt cgtgccaggg
    85681 tgccgatcag gtcgtccgcc tcatacccgg cctgccgaat caccggcaga gacagcgctt
    85741 ccaccacgcg atgaatatac gggacctgcg cgctcatccc ctgaggcatg ggcggccgtt
    85801 gcgccttgta ttctttaaag gcctcatgcc ggtgagtcgg gcctttttca tcgaagacga
    85861 cggcgacata atcgggatga tggtcacgca gcactttcat caacatcgtg gtgaagccat
    85921 agaccgcatt ggtctgcaag cccttggaat tgctcaaggg cggaagggca aagaacgccc
    85981 gatagatata ggcactgccg tcaatcagat agagagtggg catgatggag gaataggcac
    86041 acgggttatg gataactggc gcgacggatg tctacgtcga cggctgtcac tcgggagcaa
    86101 tgatcagcgg cggtttgtcg aacttggctc gcatcgcgtt gatcgtgctg agaacggtag
    86161 attgtccgac gattttggct tccacacgcc gcttgccggg aaactcgatc aatgacaggc
    86221 cgatcaacat ggtcagcacg ccctgtccgg gcaagaagag catgaggaat cccgcgaaca
    86281 gaaaaatcgc cccgacgaca tttttcacga catgccccag cagccgcaac accggatgat
    86341 ggttctccat ccaggggcga ggaatgcggg tatcgaaata gtccgccggc aaccgcatca
    86401 gaatgacagg aatcgcgatc agggttccca cgaacatgac aacggacgag acggcaaacc
    86461 aaatcaacac gctcgccggc acgtaggatt cgatctgcgc caacatgtcg ctcatcaccg
    86521 gcgcatccta gcacgggcct ttacgcggcg gaaagggtgc cgacgcccag cgggtttacg
    86581 ttccctgctc tgccagctat aattccgcac catgctgccg ccgcgatcca gcagcttcgt
    86641 gctaaggacc atccgctcct cggcttgccg cctggccgtc cttggcctga ttctggtgct
    86701 gaataccgtt gaggtgttcg accaaggagc gcaggctgcc gattccgctg cacccccaga
    86761 actacagatc gacatcagca agatcggact cggcaaagaa gtcgttatca cccagggcaa
    86821 caaagagtgg ttcatgcgcg tggaggtcac gccggaaaat tctgtggtcg tacggcagga
    86881 aaaacaggga gagcgttacc tgctcgatga gagcgaaatg cacgatcgcg ccatgaccgc
    86941 ggctgaggta gatgcggcca tcagcgactt tatcaacagt gtcaaaacca gacagaaggt
    87001 caaatagtcc ccatggccga taagccgaag tttgttctct tcgcccataa cgctacgtac
    87061 gacaagctac atcaggtcgc gacgctcggc ctcacggctt cggctatggg caaggatgtc
    87121 atcgtggttc tcttgttctg gaccatcaag aagctggctg agggcaagat cgaccacatt
    87181 gactttcctc cggaatatgc cgcctcggcc gaagaggtcg ctcgactctt gaaggaaaaa
    87241 aaggttcccc ggatttcgga gatgtttcat gaagcccgtc ttgtcgggca atttcgtctc
    87301 atcgcctgta gcgcggggct cgaatatatg ggcgtcgaag cgggtgcggt cgaacgcaac
    87361 gtggacgaag tgctcggcct tcccgcgatt ctctctttga ccgcgcaggc ggaaaccaaa
    87421 ctcttcatct gatcaacgga tgacgccgcc aattccggac aggactgatg ccgggactgg
    87481 tcacgctgca ttaacggctt tttgtcaccc cgactcgatc gcaatactcc gcaatcttgc
    87541 accggctgca gaacggagac acaggctggc aaaggttctg cccgaacggc acgagcagat
    87601 cgttgaaggt gatccaatac ttgcgtggca atttcccgcg caaggccttt tccgtttcat
    87661 caggcgtttt ggttttcacg tagccccacc ggttgctgat ccgatgcaca tgaatgtcga
    87721 cgcaaatgcc tggtttctca tacccgactg tcaccaccag gttggcggtc tttcgaccga
    87781 cgcccggcaa cgtgagtaac gcttcaatct gatccgggac tcgcccgtgg tatcgatcaa
    87841 gcagctgcct gcaaatctgc tgaatctgct tggctttggt gcggtaaaaa cccacgggat
    87901 agatggcgga ctcaaccgtc gactgggaca gctgctgcat cgttgccggt gttgaggcca
    87961 aggcaaagag ccgcgcactg gcttcagcgg tggtcttgtc tttggttcga agactgagga
    88021 ggcaggaaat cagcaccaga aagggatcgc ggccggattt tttggccacg acgccgacaa
    88081 ccgggtcagg ccagcgggca atctcccgct tgaccgttcg gatgacggca tgaatctcgt
    88141 gcgcctgcat ggaggtgccg gaaagaggga ttaaattggg acggtctaga tgactggcgg
    88201 aaggaaaaac gcctggcgtc tattcgggac gtctctttgc aagccacttc tggcggcggc
    88261 gttgagcctc tcgctctttc gccttcttgc ggacactcgg cttctcatag taccggcggc
    88321 gcttcagttc gcggaacaat ccttcacccg ccaacttctt cttggcgacc ttcagggctt
    88381 tttcaacgtt gttattgaaa accttaattt ccatcgtcag ctaccttcga ctctctttca
    88441 gcgaagtttc aggatattca acatgttttg ggaccacgaa tcgaagggcg gagtgtagca
    88501 tagcgatttc gcatccacaa gcatttgttc cgctacggca aaccgttccc gaatggatgc
    88561 aacaagtagc tgatttggtt gaactttcat gttttcttcg accatcgcca cacagccatt
    88621 gattgcggca tctgccagtt caattcccat cttcaagtca ggctgaagct ccggttttgc
    88681 ctgcgccaac agaccgcgca gcagcgggat cacctcgcaa cagagtttca caatttccat
    88741 cggcgtttcg atggcgtcca ataaaccgga ggctatttgc accgctcggt caggatggtt
    88801 aggcggaagc ttccttgcct tgagaactcg agcgtaggca tcccggtcgg cttgcgccaa
    88861 ctcgcggaag cgcgtaccaa tctccgtgag gcgtgtttcg gccggacgtg cgcggttcag
    88921 tttagccacc atggtgccta acgacgccgc gaacgatgcc gcgagggcgc ctacactgcc
    88981 gcccgtcaat ccttgctcag cggcaagaac attcgtcgat gcgacgggct tcgcgggcaa
    89041 cgacggtgac tccttcaccg gttgcgaccc ggccagcctc ccgatggctg cgcgcgattc
    89101 ggcactgtcc aatcgcattt cgaggacctg cctgccttca aatcgatcga gacgaagcgc
    89161 ccgctgcgcg gtatcgatga ggcattgctc aggaaccaga ccgataatct ccgtccccgc
    89221 cacttccact ccgcgagctg ccgcttcccg ctccaccgcg gtaaaggcca cctgcagcga
    89281 ggtttcctca tagttcgtca gattcatcga gacctggacg agcccctggc tcctcagttc
    89341 aaccccgatc gctttcaccg aaggaagacc tccactcgac tgccgaacag ccttcgcgat
    89401 cgccttggca actaacaggt ccccactctt cagattgacg ttaaaggcga tgagcggcca
    89461 ccgcgcgccg actaccgtag cgccggcggt gggatgcagc tgcttcggac cgaagtcggg
    89521 cacccaggcc gggtccgcgg ccatccggtc ggccagtccc ttcaggccac cgtttcggat
    89581 ccattccagc tggacgcgct ccggcctcgc cgccgcctgc tcgtagagaa acacggggat
    89641 tttcagctca ttgccaatgc gctggccgac catgcgagcc aattgtacac aatcctgcat
    89701 gctgacctcg cgaatcggaa cgaacggcat cacatccgtg gcgcccacgc gcggatgttc
    89761 accatgatgg ctccgcagat cgatcagctg agaagcgagt cgcgccattt gaaaggccac
    89821 ttcggcgacg gcatagggcc gtccggcaaa ggtgaccacc gaacgatgat gatccgtatc
    89881 gtgcgactca tccagcaagg ctacgcctgg gacggatcga accagggcca cgagggcctg
    89941 gatgacgtcc gggttccggc cctcactaaa attcggcaca cattcgatca cctgactcac
    90001 ggcattcctt cttctggtgt gcagggccga cttacggcca ggacagtctc agacaaaaaa
    90061 aagccggagg caggcgacca taccctccgg cttggcaaaa catgacggct tcgcatccgc
    90121 gcgcctgtcg ctgagtgcta cttggccttt ttgacaaagg ctttgtaaat ccgtttggaa
    90181 gaactttgct cctcttccaa tcccaccatt tgatggccgg tcgtctcaca ccaggccggc
    90241 atatctttct tgatgccttc gtcgtccgag acgacttcca gcacctgacc catggtcagt
    90301 tccttgatct tcttcgaggt caaaataatc ggcatcgggc agaagtaccc cagcgtatcg
    90361 agtttcacat cagcctgtat catgccctct cctcagcccg cttagataaa cagattgaca
    90421 ctgccctgct tggcttccgc caggtaggtg gtgacaccgg caaattggtc gattccgtcg
    90481 atcaacgtat ctttcgtgat tcccatcaag cccatggttg tggtgcaggc aataaagcgc
    90541 acatcgagat ctatcgccat ttgcatcaac tccggcacgc cgggcatacg attctgcttc
    90601 atgacgacct gcatcatcct cgtgcccagc ccccagaaat gaaaacggga caagggcaat
    90661 gtgtcggcgc cgcctttatt gaggaagccg aacatgcgac gaagccaatc cgacgcggaa
    90721 ctgctcgccc cctttttgcg aatcgtgttg agcccccaga aggtaaaaaa taccgtcacg
    90781 cgcatcccca tcgcggcggc gcccgtcgcg atgatgaacg ccgccatcgc tttatcgaga
    90841 tctccgctga ggagcacgat ggtgacgcgg tcagccttcg actcccggag ttccgccagg
    90901 gcggctgtgg gctctatttg tgcggtcgtc atggattacc tcgcctgtct gagcacacac
    90961 gactgaattc gcatcaaaaa tgactatagt ctccacttct tttttttgtc aagaaacgcg
    91021 aagccgtaac ggcgctcccc ggcgctcctg acgcagcact gcagacgttt ccccttgcga
    91081 tccattgctt gacctgttgc agacccgccg ttatagtcca ctcgtccgcg aagaccctgg
    91141 ccatatcccg gttatccatg agcacagacc tcatcaccga actgaaaccc acgaaaagtg
    91201 cgccgttatt cggcttctgg tatccagccg ctacgagcag ttccttgggc gtcggacaga
    91261 tgcagacgca ggtcatgttg ggattgccca tcctcatctg ccgtgatcgc caggggcagg
    91321 tcgcagccat gcgcgatatt tgtccccatc gcggaatgcc gctgtcattt ggccattttg
    91381 acggcgagcg cgtcgaatgt gcgtatcatg gctggcaatt cgacacgggc ggccgctgcc
    91441 gccatatccc cgctttggtc gaaggctccc cacttcaagc agagaaaatc ggcatcacat
    91501 cctatccctg ccgggatgaa gatggatacg tgtgggtctt tctccccgat ccgcaacagc
    91561 ccaatcaacc ggtgccggaa ctgccgcgtc tgccgttgcc atccgcgccc taccgcatgg
    91621 tgcagatctc taccatcctc gattgcacca tcgacgacgg catcgtcggc cttatggatc
    91681 cagcccatgg ccccttcgtc caccaaagtt cctggtggcg gacgcaggcc agcatgcatg
    91741 acaaagccaa aaccttcgaa cccatcccca atggatttcg gatggtaccc catgcacctt
    91801 cgaaaaacag cggcccctac aagctgctca acaaactcta cggaggcccc ctgaccacca
    91861 ccatcgactt cgtgttgccc aatcagcggt ttgagttcgt gcaatgcggg tcgatgtttg
    91921 tctcatcgcg cgcgaccgtg acgccggtcg gtgagcaaca atgccggatc gatttcaccg
    91981 ccgcctggaa tatccttccc tggctcccct tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
    92041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    92101 nnnnnnnnnn nnncaatgcc ggatcgattt caccgccgcc tggaatatcc ttccctggct
    92161 ccccttcgcc aaacccctgt tccgatattt cgcgaatatc ttcatgaagc aggataaaca
    92221 ggccatggag cgacaggctg ttggcctgaa gtacaaacct gcactcatgc tgatcgacga
    92281 cgccgacacc ccggccaagt ggtactacaa gctgaaagcc gcccatctcg cttccgtgca
    92341 aaccggacgt ccgttggagc atccgctcaa aggtccggtc acactgcggt ggagaagcta
    92401 gcaacagcct cggctgcctg aagtgggcgg tacctgacaa gacctgcgcg ctgagggcgc
    92461 taggtgagtt ggtggctcgt tccgtggttg ggctcgttca agagcgccat ggcccggcgg
    92521 atttgctccc gcgatcccag caacgtcacg acgtcgccgg cttggagccg cacggtttca
    92581 gacgggttcg attgcgtgac ctgactgcga gtccaggcga tcaccgaggc gccggttcgc
    92641 ggacgaatcg acaactctgt taacgatttg cccacggcgg gagcgtcttc gtcaacccgg
    92701 caggtttcca cctcgacatc ggtcagcgtc cccccgcgca aatgatgcgc caattccggc
    92761 atttcactgc gacgcagcag ggcatacccc tcccgcctga tttgttcagc ttttctggcc
    92821 acgaattcct gcgggagaca ataggtgcgc aataccaggg caaaaatttc aatcgacgtc
    92881 tcgaactcct ccggcaccac gtcatccgcc cccagctgat gcagttcttc cagctcccgc
    92941 aggtatcgcg tccgcaccac gatgtgtagc ttcgcattga ggcccttcgc gactttcacc
    93001 gttcttcgag ccgtaaatgg atcggataga gcaatcacga ggaccttcgc gtcctcgatt
    93061 ttcatgtgcc ggaggacatt cgcattcgag ccatcgccgt aataaatcgg gacgccatgg
    93121 cgcgactccc ggcgcaccgt ttcgccatcc agatcgagcg ccacatgagg gatttcggtt
    93181 tcgctcaaca cgcgggcaag attgcgacca ttgaggccgt atcccacgat gatcacatga
    93241 tccttgatcc ggacctgccc cccctccaac tgctcgacat gcgccaccgt gcgggtgggc
    93301 atccagccac gaactcgttg cagcgcttcg atccgccggc ccaggtgagg cgaccattgc
    93361 atcacaaacg gcgtcaccac cattgtgagc acggacacag caagaaataa ctgatagtga
    93421 tccccggaga acaagccggc ctcctggccc tgctgcgcga gaatgaaact gaattctccg
    93481 acctgcgcga gcgcaacgcc gaccagcacg gccgacctgg gcggcgcgcc ggatgccacc
    93541 atcgctccgg cgccggccag cagcttcccc gcaatgacag ccaccagaag ccccagcaca
    93601 atgaacggat ggtccaacac cacccgtaga tccatcaaca cgccgataga cacgaaaaac
    93661 aaactattga aactgtcgcg aaacggcagc acctcggcca acgcctgatg gctgtactct
    93721 gattcggaca tcaccaaccc ggcgataaat gcgcccaagg ccagggacaa tcctcccaac
    93781 gacgtcagcc aggcaatacc gagacagagc acgataatgg agagcaggaa gagctcacgg
    93841 ctgcgacttc gcacgatatg ccgcaataat cgcggcacga gataccaggc cgcagcgacg
    93901 atcaacacca ctacgaccgc cgccttgagc agagtgagca acaccattcc catggcactc
    93961 ccgctgggcg tcgccaggat cggggtgatg agcatcatcg gcacgacagc caggtcctgg
    94021 aaaatcaaaa tggccacggt ggcacggccg tgaaacgaat cactctcgcc ctgctcggac
    94081 agggctttca gcacgatggc cgtactactg agcgacagga gaaagcccca gaagatgccc
    94141 tgctgatagg agagccccac ggccaacgcc ccgagcaggg ccaggatgag cacccctccg
    94201 gtttgcaccg gcgcggccac cgcgagaatg cggcgggctg aggcaaagtg tgccgaggaa
    94261 aactccatgc cgatcgtgaa gagcaataag acgatgccga tttcggccaa cacctgcact
    94321 tgagacacat cgggaatgag atgtaaccca tgtggaccaa tgagcgcgcc cgccaccaga
    94381 aacccagcga tcgacggcaa ccggaactgg tgaaagacga acaccactgc ggtcgagacc
    94441 gcatagatca ccaataaatc gccgagaacc gcgtaatcac tcatagagtc acaagatttg
    94501 gacaatcagt tacatcggag gttggacgca ttcgtgacgc agcataccgt agggatctca
    94561 atggaacaag gagttctgct tgtcccgcac caggaaactc agctaacgtg acgcgcttag
    94621 cggcacattg gagagacgtt acggagggat cttgcgcgcg atcattttcg acttcaatgg
    94681 cgtcatcgcc gacgatgaga tgacgcacct ggaatgtttt cagaaggcgt tgcgcgaggc
    94741 tggactcgtc ctgagcaagg aggactatta cggaacctac ctcggcatgg atgaacggac
    94801 ctgcgcagcc gcgatgctca ccagacggga cggcacttgt gaccacacca ccctggcccg
    94861 aatcatcgaa cgaaaagcgg cactttttcg aacccacaca gcgacgcaca aacccgcatt
    94921 gtttccctgg gtgaccggct tcgtgcagcg ggcagttgag cagtaccggc tggcaatcgc
    94981 gtcgggcggc aggagagaac agatccagtc ggctctcgcc ggtcattcca tcgaggctac
    95041 gtttgaggta atgatctccg ccgatgactg cgccgtcggt aagcccgatc ccgcgattta
    95101 cgaatgtgcg cttcggcaac tcgctgccaa gcgacccaaa ccgcccctcc tgcgagccga
    95161 tgagtgcctg gtgatcgagg attcccgtgc aggcattcag gcgggcttac gggcagggat
    95221 gcgcgtgctc gccctggcga caacctaccc ggcatcacag ctgaccgaag cgcatcttgt
    95281 cctaccgagc ctggaaggaa tcgatccgtc agagttggcc cggcggctgt tctaaccagt
    95341 gatgagaaag tcttactcga cagggcgaac agaaccggca ggattagaag ggaatgcgaa
    95401 tgcccatctg aaactcgttc ggcatcatgc ttttgcccat agcctcgccg aagagatttt
    95461 gctgcggcaa ggtcggctgc ggattcaagg cccgatcacg ctccgtcacg taccccccac
    95521 cgaacccggc gccgatgtat ggaatcaacg tctgttcgct gacacgtaat cgcccgctca
    95581 acacaggcga ttgccggaac aggtccgcgc gctgttc
//
LOCUS       DAOQDS010000004        87570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_77_length_87570_cov_21.420819, whole genome shotgun sequence.
ACCESSION   DAOQDS010000004 DAOQDS010000000
VERSION     DAOQDS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 87570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 87570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87570
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77_length_87570_cov_21.420819"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..319)
                     /locus_tag="PK589_01530"
     CDS             complement(5..319)
                     /locus_tag="PK589_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ18301.1"
                     /translation="MLTVKELSAWLNIKESTLYLWVSKNKIPCRRIHGLVRFEPEAIQ
                     AWLKSFETSPGKPFPLPTRDDTRDLDHLIEAAKCEVYTPRHGETRPKSGLIQKEDADG
                     AV"
     gene            complement(363..1370)
                     /locus_tag="PK589_01535"
     CDS             complement(363..1370)
                     /locus_tag="PK589_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HNJ18302.1"
                     /translation="MDSSFTLTIDWLAFTVLASNPQETMKVLGGDWSKAKGGFRGYPL
                     SWMRADGLRGVGKLGTNAPRRPNEIHVDLSGGLASALTLDQIRTLLKWVHKQQGHVTR
                     IDCALDDRAGAVPVSTIREAVSAGQCVTRAAQVRHIVSNLTHGTGATTGETMYFGSPQ
                     SQTLLRIYDKRLELQSKGQENWQDYGTRWELELKKDRAEQCARALATLDEADWKELVI
                     GLLRSYVDFRDITKETEDEERYRAPVLEWYALLTEGFQKGRLAQEKQVQTLQNVKRWV
                     SDTLTPMLAVICATPGGEEWLLNEIVRGIARWKDRHRNLLKQPPTRFHRSAGGHAGSP
                     C"
     gene            complement(1516..1764)
                     /locus_tag="PK589_01540"
     CDS             complement(1516..1764)
                     /locus_tag="PK589_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18303.1"
                     /translation="MKTDNTKTETLQFKVTEQERELIEKCAADEGTTVSKYVRGAVLM
                     SMVMDGKAEAIKIVAREVGEKAFAVVRQKLTRTTQEGR"
     gene            1902..3290
                     /locus_tag="PK589_01545"
     CDS             1902..3290
                     /locus_tag="PK589_01545"
                     /inference="COORDINATES: protein motif:HMM:NF037255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNJ18304.1"
                     /translation="MRDLFPGYYLPSEVEFKKLWREGLFILDTNVLLNLYRFPDKARA
                     DFLNVLRHIAPRLWIPFQVALEYQRNRLSVMSEQRKAYDEVEDIISKSLKQLRDKLST
                     LNLPTRHPSINPTQFLQEVNVLFERYAEELRTLKQKERELTSEDPIRDAIHELLGKNV
                     GSRPSNQEELDAIFKEGEDRYQRRIPPGHLDADKSMDKGGNRFVCGELVYQKEYGDLI
                     LWKQIINYARKEGKKYIVYVTDDSTDDWWHIVDKKTIGPRPELVEEIKQKGGVELFYM
                     YGTERFMKFARTYLQVSVPPESISQVSATVHDEQRKRAIQDFAEKLFLPQPNYEDVVR
                     NWLRARHPGDRMSVGLVPGDPDIVFTTRDGQTIAYEVKVLTAIGPKEVERLISSMGGM
                     KYLHNEVHIVIVARSTQAKAAIDKIEDWIKKASRFYRSLPDSLIAGTVENFGEGYEFF
                     PVTILMAGSIGD"
     gene            complement(3476..4546)
                     /locus_tag="PK589_01550"
     CDS             complement(3476..4546)
                     /locus_tag="PK589_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNJ18305.1"
                     /translation="MIQFTRGDILRSESEALVNTVNCVGIMGRGIALQFKNAYPENFK
                     AYTVACKLGQVKPGRMFVFETGQLTPPRYIINFPTKRHWRGKSRIEDIEAGLVDLVAV
                     IRAKKIRSISLPPLGSGLGGLNWDEVRSQIQSALSPLVDVNVTVYEPQGAPSSDTMQH
                     RREVPKMTASRATLIELVQRYLDGLLDPYVTLLEVHKLMYFMQEAGEPLLLKYRRAIY
                     GPYAENLRHVLHAIEGHHISGYDDGGDVPDKQLTLVPGAVEDATEYLANHPETRSRFD
                     RVAALVKGFESPFGLELLASVYWIMRNESVHSLEDVVERTYAWNERKRNFTERQIGIA
                     ANVLIRQGWVNRELRSESLPHN"
     gene            complement(4562..5197)
                     /locus_tag="PK589_01555"
     CDS             complement(4562..5197)
                     /locus_tag="PK589_01555"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HNJ18306.1"
                     /translation="MPAQPKIYHIAHVDRLPSILADQFLWCDREVQRREPVGTTIGMN
                     GIKQRRLNEIRLSSHPNLYVGDCVPFYFCPRSVMLYLINQSNHPDLNYRGGQGPIVHL
                     EADLRSAVAWADRNRHRWAFTLSNAGSYFFEDRCDLNRLHEVNWNAVEATEWRSCKDG
                     KQAEFLTEHSFPWHLVERIGVQSRSTYNQVVVALSPSGHRPSVEIHPEWYY"
     gene            complement(5952..7040)
                     /locus_tag="PK589_01560"
     CDS             complement(5952..7040)
                     /locus_tag="PK589_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNJ18307.1"
                     /translation="MPSTAPIPPHARPPRIASIESYRVVAILLIVSLHTNLIARLHLI
                     GGGVGFLVDMPLYLLFWISVPYFFLTAGYFYGRTVDAGAAPFSLLRRSCRSLTLLFVI
                     WVAVYSIIGPNWISNVYTQGLWSTISTEASHTMGRLMQEHVTLLLVPRPPIFHLWFLP
                     ALIAGVSTVAMVVTSRLKTWVGALLVSTYILLVVSAVVSLPQYSPSLLLLGVFFTLLG
                     WWISQQAAFTGSFALALIAGGFVVAMLEGVALKRVFLASPHLVMDYPYAGAVLLVVGI
                     FLLTLAYRTIGENTILPFLARFTLGVYVSHILVGYTLVPLHDQLPALPIIWHVLYTLL
                     VYGLSVLFTWGLSKIPWVRLSVTRQAAA"
     gene            7474..7986
                     /locus_tag="PK589_01565"
     CDS             7474..7986
                     /locus_tag="PK589_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HNJ18308.1"
                     /translation="MKKQGILVLAGLLIGLQSGTAFAVNPDNGPGCGLGKLAWSDYKT
                     PKNIAPQVMMATTNGTFGSQTFGISFGTSGCTNDGKVMASQETNVFVASTFDTLSTDM
                     ARGGGEHLTALATLMNVPVEQQPAFFQLTQQQYLALTEAGEVSSVAVVKALQEAMTGH
                     PTLAQLAGTR"
     gene            8185..9351
                     /locus_tag="PK589_01570"
     CDS             8185..9351
                     /locus_tag="PK589_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNJ18309.1"
                     /translation="MTQPSHRLLLLTIIILGIGLYTLDLYLPLGIGNGVLYGGLVVMS
                     LAIPDRKIPLLVAGTCSVFALSDVFLGVTLPNVPLWMGLSNRMFSLTAIWFPVVYAFQ
                     RRKSEEALRNAHNELEIRVEARTRELALVNQALVGEIEERMETERSLRASESSLKVSQ
                     EELRQSREELRALAGQLLTAQEVERRRIARDLHDDVNQRLAMLAMDLRRIEKGEAGDL
                     SNIGGMVKSISGRLTAVSDDVRQMAYRFHPSILDDLGLMKAVRRLVDDFSASTGIEAV
                     YVYQDCLSPVPTDLATCVYRIAQESLNNVARHAKATEVEVELICDEGMITLSVRDNGV
                     GFDAVHASQGRGRLGLLSMKERVRLVRGTLNVSSSPGGGTHIEVGVPLSGSQHV"
     gene            9344..9988
                     /locus_tag="PK589_01575"
     CDS             9344..9988
                     /locus_tag="PK589_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ18310.1"
                     /translation="MSKPRVLLADDHSLVLEGFRRILADQCELIGMVEDGRALLEAAA
                     RLEPDIVILDVSMPLLNGIDAATQLKKTHPSMKVIFVTMHSDADYVRSAFEAGASGYL
                     LKRSAVDELEQAIRAVWAGHSYITPLIAKDLLDVLLTAGPGQTPQKKTLTFRQREVLQ
                     LLAEGRTVKDIALRLKISTRTVEFHKAQVMEQLNLRTTAELIKYALTHGLLAPS"
     gene            10233..10877
                     /locus_tag="PK589_01580"
     CDS             10233..10877
                     /locus_tag="PK589_01580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ18311.1"
                     /translation="MRIAQISQCFEEVSNESERPEVRGVEFVTRSLMRFGHDVTVFAS
                     GDSRVSGRLIPVSPQAIRHYPSPKRHLSEGLTFLAIEKAFSMESSFDLLHVHAGFVAF
                     PMMRRSPLPSVATVYGSLDAPEILQLFRAFRELPLVATSAEQIRQCPELNWLALIPVP
                     GPRQRSEVPAETMDSLVETATAYTAVYESIVERAVRPTHAVRPAYSLYDVQTTV"
     gene            11233..11448
                     /locus_tag="PK589_01585"
     CDS             11233..11448
                     /locus_tag="PK589_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18312.1"
                     /translation="MNRNDFLVALFGRPVWFHCKQCHRVMAGGTEESFPDMWQEEAVA
                     MEAWRVRPEDLWWFDTVCGSCQEQQRS"
     gene            11566..12681
                     /locus_tag="PK589_01590"
     CDS             11566..12681
                     /locus_tag="PK589_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18313.1"
                     /translation="MPTGLPPGLLRVIDQIVCVAGTSGRGTDLWDGIRVECEREYSGI
                     RHGKGTLAHALEWEILKLQTRLESSAYGNPVVPVPLFRNRHVHIGSLIRLWRRLALET
                     EEWLASQGYDTLLDVGPWGGFNFVLEPDGYTRMPFARLTLAVGGLPSTPFHEQGGPLF
                     QHLLPRYHAELQQAGVNFPPLWQYHHAKHDASVRLREVSATHYLPTHSYERRTFVKVR
                     VSRDCEAVEEIALQDFLLLLERLQFTTDWELYREQTKDVDARFDLQDFISLNHMVEGV
                     YQRTDAEDRLLHEMKDAFRGAIRARQVLYGYLSQVVKLRWVENLYWALAEAALGVKRY
                     QRMVSFDREVCPQIPPRLLIPVRRHLNRYHNRVGGTA"
     gene            complement(12701..13672)
                     /locus_tag="PK589_01595"
     CDS             complement(12701..13672)
                     /locus_tag="PK589_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNJ18314.1"
                     /translation="MTYQQLMSLANGYAESKTLLVANELGVFTAIGTGGRSAEELAVS
                     CGTVREGMKLLLQSLTGLGLLHLKAGRYWNTPLGRTFLDGGSPLAITNLLWLLNHHWV
                     DWSGMTKAIRRGPPGWAPVTKTANFRRRFALAMQERSHILVQPTIDSFRLPRQAERFL
                     DLGGGAGSYAIALARRYPTLQGLVIDQSVTVAKRLIRRERLANRIQVQEGDLRTLPLP
                     TDLDVVLLANVLHDFNKTENRHLLRRTRKALRPGGRLFIVEYFLAREETRPAEAAVFS
                     LLMFAFTGTGRCYSWNEVEAWLRQAGFGRCRRHKVTGSIGTLEATKL"
     gene            13849..14601
                     /locus_tag="PK589_01600"
     CDS             13849..14601
                     /locus_tag="PK589_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HNJ18315.1"
                     /translation="MLDVIGLGAVAGLIPVYLGIFAALYLGKVLPRTWEGGLIGVANG
                     VLVYLFFDLMHEATEQTGARDPLSWLVFLGSLGVSFVGLVALESSQVFGARSGNRILS
                     LPYMIAVGMGLHNLGEGLAIGASYAGGEYTLSALLVAGFGLHNGTEGFGIVGAAGKTP
                     ISWRDVALLGLIAGLPTCVGTFLSGQGVSSYFSICFYALAAGSLLYVVLSLTVMSYTA
                     TRRVQVSAGMFTGIAIMYLTAMILTLVSGVRS"
     gene            14817..15920
                     /locus_tag="PK589_01605"
     CDS             14817..15920
                     /locus_tag="PK589_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNJ18316.1"
                     /translation="MAGREFHDGAKDGLEALEPLDPEKIHSFTELLEAMRKTAFGGRR
                     LGEAYETLAAMIEDPDCKVVLTLSGAMTIAKMGKIISTMIDRGMVQAIVSTGALVAHG
                     LSESVGKLHYRHEPSQTDEELFQKGYNRVYDTLEMESNLNYVEHVVSQTMKRLNVDQP
                     LSSNFLTRELGKTLAEEFEGPGILKSAYLKNVPVYIPAFTDSEMGLDLGTWAMGKQID
                     FTRSQIKEGGDVAVLRALHQTCPVFNPYLDLNHYAEEVLGSTRLGIFTIGGGVPRNWA
                     QQVAPYIEISNTRLGLNVEPPRFHYGVRICPEPDYWGGLSGCTYQEGISWGKFVPPAE
                     GGRFAEVLSDATLVWPLLMVGLLERFAAKSVSA"
     gene            15917..16570
                     /locus_tag="PK589_01610"
     CDS             15917..16570
                     /locus_tag="PK589_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNJ18317.1"
                     /translation="MTPLTGWMIRFATPFLLLVLTTASALAASSPAVDNRMRGKADAP
                     ITLIEYSDFTCGYCLKFFKETWPKIHARYVETGKVRFLYKDYPRADQGPGVTAALAAR
                     CAGDQGTYWPMHDRLFAADGRLDVDSYFQHAKAIGLDQSKFRECLRDAPHMKAIFQDR
                     DEANAWGFHGTPGFILMRTTQQPTAQNPALAIPGAFPFAAFEEEIEKLLAASGEKAK"
     gene            16650..16928
                     /locus_tag="PK589_01615"
     CDS             16650..16928
                     /locus_tag="PK589_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18318.1"
                     /translation="MGSKQAFWTVPAKDGEPDLWVCMSCLSEAFCRKVPMPACPTCHG
                     VSTYEAFTLAAVQDWGTEELIAKAVAAQQEEAALSAAASAAPPVESVQ"
     gene            16956..18386
                     /locus_tag="PK589_01620"
     CDS             16956..18386
                     /locus_tag="PK589_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNJ18319.1"
                     /translation="MENGRPFLIGGRWSQSTTVAPVRNPYTGETIAHVCQASPADVES
                     AIQSSVDGAAAMRRLSGYARSTLLANAAQALQARQEEFARTMTAEAGKPLTDARREVG
                     RAIQTLSIAGEEAKRIGGEVVPLDWSPGMESYWGVTRRFPIGPILGITPFNFPLNLVV
                     HKVAPALAAGNSILIKPAPQTPLTALLLGDLLLKAGVPPGGLNILPCDNTLAEQLVVD
                     PRFKLLSFTGSAPVGWMLKAKCGKKKVVLELGGNAAVIIEPDADLEYAAQRCVTGGFT
                     YAGQTCISVQRIFVHESIAESFTEQLVARVRALATGDPGDERTVVGPLIDAGAAQRIE
                     GWIDEAITQGARLLSGGSREGSVVRPTVLSQVTAGMNVSCREVFGPLVTITPYREFEA
                     ALRAVNESDYGLQAGIFTTNIGRIFQAFEQLEVGGVLANEIPTFRADHMPYGGIKDSG
                     IGREGLRYAIEDMTEPKLLVVNLRRP"
     gene            18562..19137
                     /locus_tag="PK589_01625"
     CDS             18562..19137
                     /locus_tag="PK589_01625"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HNJ18320.1"
                     /translation="MVPTHMFLTRGVGVHKEKLAAFEQALRSAGVAYCNLVSVSSILP
                     PNCKILPRKRGEKLLNPGEITFCVMARSETNERNRLISASIGLAIPTDRDTYGYLSEH
                     HAYGETDEESGEYTEDLAAQMLATTQGIEFDPDVAWKEREQVFKMGGKIVRTLNITQS
                     AVGRPNRWTCVIALAVFIPTENIPKSLRNKA"
     gene            19146..20078
                     /gene="speB"
                     /locus_tag="PK589_01630"
     CDS             19146..20078
                     /gene="speB"
                     /locus_tag="PK589_01630"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNJ18321.1"
                     /translation="MALPSGWLGQADNFLGIDEPWCHPDQAGVYVLPAPYEHTSSYIL
                     GSDRGPSAIIEASQQVELYDETLRYEPYREWGGVATANTLNLNGRVDGQAVQAIQDFV
                     QPHVGRGKFLVTLTGEHTGALGAIRAHAQHYSGMCVVQIDAHGDLRQAYQDNPYSHAS
                     VMARVVQDGLPLVQVGIRSISPEEIELIDKTPRIKTFFAASILDPSGPYEGRAARWIP
                     EVLASCPGPVYLTFDCDGLDASLVPALGTPEPGGLGWYDTLALVTALANGPGIVGMDI
                     SEIAPIEGFVAPQFSIARLIYRMLGRVRAGRRVH"
     gene            20175..20885
                     /locus_tag="PK589_01635"
     CDS             20175..20885
                     /locus_tag="PK589_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNJ18322.1"
                     /translation="MRINKFFTEQGLCSRREADRLVAEGRVTINGVVAKLGDQVSSQD
                     VVARDGTILQRGNRAVYLKYHKPVGVTTTTELHVTRNIISEINHRERIFPIGRLDKDS
                     SGLILLTNDGDIVNEILRVEHGHEREYRVEVDREFDDAFLSRMAEGVVILGERTRPCL
                     MARVGPRRFRIILTEGRNRQIRRMCQALGYRVVSLHRVRIMHITVEGLHAGQWMDLSE
                     EERRRLFQALGRPPESSL"
     gene            complement(20899..21675)
                     /locus_tag="PK589_01640"
     CDS             complement(20899..21675)
                     /locus_tag="PK589_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HNJ18323.1"
                     /translation="MSFASRFKLSFPSTNQLIISLLIAGLGWISGQALSRIDQDLRIM
                     YTEYTLGAADLAHISADVIRYRNTIIRSLEAENQAVFERITESLPAQRARIQHAVDRY
                     AAAGLRVSRSGRSEEKDIQAVRESLDQYFHVASKTVDLLSQEWKAGTPGEATELRRKA
                     EIHAADNGGPKVMQVSLALDRLLETVAEVAKDMRDEGTKTIRSTSYWIVGGSFFIALL
                     NLFLSRTTRAPLSASRPEESVRPDSLDLPGGGSNTALRAE"
     gene            complement(21791..23467)
                     /locus_tag="PK589_01645"
     CDS             complement(21791..23467)
                     /locus_tag="PK589_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ18324.1"
                     /translation="MNPIATESTITTPSAWTILSRLNLLVGLERRILAIIASYAVVIG
                     LFALIVPLTVQELVNTFAFAIQPIMIVTLVAIMLGALLLMGAFRVLQGRAVEILVQRI
                     YTRLAVAFTEALPRFRENVFLPQHTNTFIEAELLPRALVAMLVDIINVFVSGMIGMTI
                     LIMYHPYFLGYNIFLITGFAFLLTFFGRGGLRITQRVSNLHYQTFHWLQDIGINRLHF
                     KSTDSLPLLLKKTDALVQAYVMARKTRSDILTGAQYKSTVIFQAIAHSSMIGLGGWLL
                     SLADITLGQFVAAEVIVGTLLLNLDIVARRMYAAIYVATSMQELSRLFEMPKEEVSGP
                     IAAWLPNPTLQGVRLTCKDVSFASPEGPMLFDHFNLEVLPGEKISVLTGTSKSKTSLA
                     LLLAGLYHPTSGVIRYNDIDLRDVSLNYVNRCRGLMLDSHPTLFDGTLEENVTLQRPS
                     IQFADLSWALRFVELEEEIDALPLGLETVVHGNGANLSRSQVLRILLARMIVTRPELL
                     IFNGSLHNIDPTVRLTLLRRLCAKEEPWSIVFVSNDPEVGQLVERRVILD"
     gene            complement(23610..25277)
                     /locus_tag="PK589_01650"
     CDS             complement(23610..25277)
                     /locus_tag="PK589_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ18325.1"
                     /translation="MSQDHSDNQSNLFQAVVGHLGLLFRLERRILGLIVSYSIAIGLF
                     SLIVPLTVQELVNTFAFALQPITIVTLAAVMVAGLLFVGAFRALQYYAVEVLERRIFA
                     RVAIAMAQQLPHLRYQGFKPRFANYFVETILMQRAVSVLLVDLINVIVGGAVGMTILV
                     FYHPYFLLFNAILLVGFNVVFFLMSHGGLKADMDMSHAKYDTLHWFQEIAYNLLHFKS
                     TDSQALLIKKTDQLLDNYVGVRRTRFGILIRQYLGSLGWQAIVHSGLIATAGWLLGIG
                     QLTLGQFVAAEVVVSGILSSFDGVVKRMGHIYYFLTALTELSFLFSLPKDQDAATLSI
                     PLPDPTVHGIRVTCKDLTFAPAGGPPVFEHFNLEVTPGEKIGIYADTTMAKTALARVL
                     GGLESPTAGVIRYNGVDLRHLNLDSVNRFRGFILDSQLSLFEGTLEENILLGRDYIPY
                     SDVRWALRFTELEEEVDALPHGLKTHVRSAGKIFAPTHIVRILVARAILGRPQLLIFE
                     GILHNMHPAMRETILRRLCSKEEPWSVIFVSNDPNLTPHVDRRIMLN"
     gene            complement(25410..26900)
                     /locus_tag="PK589_01655"
     CDS             complement(25410..26900)
                     /locus_tag="PK589_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ18326.1"
                     /translation="MKNYAWILVIAACALWGLPESSAQAEDTGKAKTLPPIPLSLNEV
                     HAWIDRSHPLLKGAGTEKTSARGKMLKALGAFEPVLVNDTEIERFIDKSTSKTQSVGF
                     NDTLVEARTPWGFRGSAGVRQAIGDAKIPDLSFGNGNQQVILGGFLPLLKGLMINPEN
                     AELQRSELADPRAEVKISQTRQDLFLAAASQFWDWVSAAKFVDVQRRALGVAEERLRQ
                     VEGRAKAGAVAPLDVVEAGQEVQRRREISIAAQRAVEQEQYKMSMFLWENQTPTTPQL
                     ERVPDFPPPAAAPTADVVKADKLQAKSDRPEIREVDIEAKVNNIDLELAKNNLLPSLD
                     LEAAPARAPEKFVLGLGYRFGAELRIPILQRRSRGEVMEAQAQADRLVLVQKYREQQV
                     VVDVDNAVSAIERAKERVAAAAESLRMAKTLEEGERFRFSLGATSVLFVNLRERNSVD
                     SEMQLVRAKADYQKALALYQWATGSWARSQPSAVPVNYRVGASLSK"
     gene            complement(26989..28425)
                     /locus_tag="PK589_01660"
     CDS             complement(26989..28425)
                     /locus_tag="PK589_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNJ18327.1"
                     /translation="MQRIREGLKTRFRSLVTQRTGLDEMAVDDLATSTKLQSWEAVQI
                     PERLRFSSRLAILLVGFFMLIVAFVPWTQTITVTGQLSAYTPFERPQDIEAQITGRIR
                     KWHIFEGVRVKAGDVILELDDYDPNFMAPDLLNLLEQRKQALEDTRKAALSRADQLDK
                     RIKEMQNLVKAAVPSAGARVLEAESKVREARQKIEASKIAVATAELNVERHQQLAQQG
                     LVSQRELELTIQAAIASKADLQGAQANLAAAEQGMKALSFGREQVSAEVLQRLLDAEA
                     ARDASIGEAARAADQVADVSLRLSNANQRRVASRILSPIDGTVVKMAQAGAGETVRPG
                     EKLVRISPNSVDRAIEMVADGIDAPLLNVGRKVKILFYGIPAIPLPAWPEMMAGTYNG
                     VIKVIDQVDDGKGNFRFWVVPDLEERPWPPQEHVRQGTKAMGWVILNRVPLWYELWRR
                     FNLFPPDYQERPPSLIDTLLPKAGRGAK"
     gene            28599..29153
                     /gene="purE"
                     /locus_tag="PK589_01665"
     CDS             28599..29153
                     /gene="purE"
                     /locus_tag="PK589_01665"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNJ18328.1"
                     /translation="MPKKKRADRALVGVLGGSTSDFPILEKAVAMLNELGIPNELLVV
                     SAHRTPDRLFAYAEQAVARGIQVIIAGAGGAAHLPGMVAAKTLLPVIGVPIPTEHLRG
                     FDSLLSIVQMPRGIPVATVAIGGAENAAILAAQILGVQSASVRQRLERFRAAQTQKVL
                     DSQDDARLSPPFPMSRGTRTSRRP"
     gene            29150..30316
                     /locus_tag="PK589_01670"
     CDS             29150..30316
                     /locus_tag="PK589_01670"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNJ18329.1"
                     /translation="MNVTVIDPGATLGVLGGGQLGAMFATAARRMGYAVNVWDPDPEA
                     PAHRIADHSLIRPFTDVDARKEFTQRVRAVTYEWENVPADLCEQLECELPVRPSSRVL
                     RVIQDRIEQKTFLRSRGLPVPVFATLASPEELGRQTMPAYPLVCKTATAGYDGKGQWK
                     ILRAEDCPAVQQELARTRRPGSRWILEEWLPFQRELSILVVRGADGASQTYPLVDNVH
                     EEGILRQTIVPADVPQPVADLAGTIACDAVQALDGVGVFCVELFLMADGRLVINEIAP
                     RPHNSGHYTLDACTVSQFEQQVRTLCGLPLGEIRLLSPAVMLNLIGEDVRLVTHSPAA
                     QDLLRVPGAIVHLYGKRTIRPKRKMGHISFLAPTRAEALTAASTFRSRFAPIHS"
     gene            30571..32430
                     /locus_tag="PK589_01675"
     CDS             30571..32430
                     /locus_tag="PK589_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ18330.1"
                     /translation="MSVPLSSDLDQTGRHPTMRGLGRLFSHSRVRVMFLQSLVAGILS
                     YELLVSNETIYGQNVSRMVALGLMLISMGIILLPKAALESTWFPGALISINTLLVTGT
                     IYLSGNASSELYLTYFLLLLIASSAPSLKQLLGLSVIICAGYGILVYEHAMQSGTLAV
                     GHLLGIPVLLIMSVFYGLTLETVAVERRRNRLLREDVQGLKQSEQVLEERRTQLETRV
                     QGLKQGLSRANQEIRQGMVERTGLERQLREAQKFEAMGRLASRLAHEFNQVLAVIGAQ
                     TGAIVSKLKPDDPLHGPVEDIFRSGERAATLTAQLLALGVHETSIRDTLSLGEAIVSM
                     REALQALLPGRIDVRVPDESKPVLVEIGHDRLECLLLHLVANARDAMPSSGRVDISLE
                     VVTGELLPAEQLEKNPRKRMARIAVSDSGGGMNLDVQAQMFEPFFSTKETHAGLGLTL
                     VYGIVRQCGGAVEVQSQPGQGTTVRVYLPLVERNAVLRDSRVLPEALSKGAETVLLVE
                     EDEIARKLALSTLHGHRYHVLEAGSAVEALLVAQQYQGPIHLTVSHLSMPEISGRELA
                     KRLVLQHPKMKALFVSGFSDETIASHRVNRKYFLQQPYRQHDLAGKVRELLDV"
     gene            complement(32447..33151)
                     /locus_tag="PK589_01680"
     CDS             complement(32447..33151)
                     /locus_tag="PK589_01680"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNJ18331.1"
                     /translation="MLPLSDDNPTERTPVVTVAFIVACSLVFLYQSSLGSTSGEAFVY
                     QYGAIPSLVFGQAVLPPELTRIPAYATVLTSMFLHGSWMHLIGNMLYLWVFGNNIEDI
                     MGHVRFIVFYVGCGILAALSHALTDPTSTVPMVGASGAISGVLGAYLLLFPHARVLLI
                     APVVGATYVPAGIVLGLWFVMQLLNGGASLGSQGGGVAFFAHIGGFIAGMVLIGFFKR
                     PEVRFFKPGRTESFRY"
     gene            complement(33174..34031)
                     /locus_tag="PK589_01685"
     CDS             complement(33174..34031)
                     /locus_tag="PK589_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNJ18332.1"
                     /translation="MSAATMRTLSSSPPTLILYHAECADGFGAAWAIWRRYPDAEYRP
                     VKHGEAPPTNLAGHHIVMVDFSYNRSTLEAMAKDAASLVVLDHHITAEQALADLPYAY
                     FDLNKSGAVLGWEWAHDEPAPWLLRYIQDKDLWHWALPNSREISAALASHPFDFELWT
                     RFEQRELEREGRAILRYENELVTKLASHATLVEFEGATVPSVQSSVLTSQIGERLSAE
                     HPFCVIWHDRNGRRYFSMRSREEGTDVGAIAASFGGGGHTHAAGFSVPLQADGLPQAN
                     PRLPRPAAS"
     gene            complement(34126..35181)
                     /locus_tag="PK589_01690"
     CDS             complement(34126..35181)
                     /locus_tag="PK589_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNJ18333.1"
                     /translation="MKTPAARSKPVPTKDEILTQITTLLDRPDDDVQAALMKEILAGV
                     IRLSESQLDVLDLKIVNRALKELRHAFRVFQGYRDRLKISVFGSARTPADDPNYQLAF
                     QFARRMVEEGYMTITGGADGIMRASQEGAGREHSFGVNIMLPFEQGANAVIADDPKLV
                     TFKYFFTRKLMFQKESHAIALFPGGFGTHDEGFEIMTLVQTGKSDPKPIVCLQAPGCD
                     YWNHWNSFITDQLLKRRLINAEDLSLFTIVDNVEDAVTEIRTFYRRYHSLRFVGRQLA
                     IRLKTPLTAEQVQEVQHQFKDMLTEGTFEQRPSLPEEIDEPGLKDLARLTFSFNRRNA
                     GRLRQLINHLNHLPATT"
     gene            complement(35283..35993)
                     /locus_tag="PK589_01695"
     CDS             complement(35283..35993)
                     /locus_tag="PK589_01695"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ18334.1"
                     /translation="MMTPGVVQIPGDVSVSEAASLLDREQMPCLLVKDGEAAFGIMTS
                     GDIVKKVVAQGLEPHDVEVRSIMSKPVHSVECDQILDDATSVMASTGTSLLIVTKQGQ
                     PVGILTARDLALAPKRCETCLPATIRVTNGEGEGAKHTATIRQLSHVGASIESRTLLL
                     PGTSIVLSFILPGTDLSFSLQGTILNSNYEPEFHEDRNVLGTYSGIDIQFLSLSSSDE
                     SKIRAWVLQNLPRASDLS"
     gene            36254..37732
                     /locus_tag="PK589_01700"
     CDS             36254..37732
                     /locus_tag="PK589_01700"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNJ18335.1"
                     /translation="MKSEFTERAHRLPAHGLGLSVDVYSPDLLELVRALRDAALEPDY
                     LEIFKATTSAQQWMRQQLPDLKLTYHGEGLWSTQPEFCRSGSGRQGVAEACAQLAALR
                     SAWLNHECATKQMAGYAFGTYLPPLYTELSARMTAENVAYLQSQVDQDAHRREIDPAL
                     VLLEMPPLTYFGCGALPIPDFFRAVTDQASCGLVLDIGHLWTVYRYTGSWQRQRLEEF
                     AAQFLDAFPMERVIEIHVAGLAELTVPHPSGIDASGNALPYWIDAHGAPIPGVLFDLL
                     AQVLSHPGLTSLKGVALEVDTKPIAMIVEEFKQFRERFGTTVQAMLHRDSPAPVQPQR
                     SPNARSAERRQLTSEEEATLQQQYLHYVQLVTTQDDPSSIRNLSLLGGSLDDLNRYRE
                     TYLPHELLHWGGELQDMFPDTCRALTESDVPLDRFVAVWFSKPRPEQDDYDFFLLKID
                     RFVEFATHACPMAAMIVMREAEELRAAYRQANEPIGSSQVRA"
     gene            37729..38913
                     /locus_tag="PK589_01705"
     CDS             37729..38913
                     /locus_tag="PK589_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNJ18336.1"
                     /translation="MNFWTTLPQPILGLAPMDGVTDAAFRRVVASQGRPDITFTEFTN
                     VNEICRGPDHLLSSLIYSEAERPIVAQLYGKDPEQFYRAAQVVCELGFDGLDINMGCP
                     SKSVAASGSGAALIKTPDLAHAILRAARQGLEDWAGGQSIHQQGFKPSRIEFIHELNH
                     RRSGSPVVPRRLLPLSIKTRLGFDCVVVERWVEHLLEVRPAAITVHGRTLQQMYRGAA
                     DWTAIAEAARVARDTETLILGNGDLNTLVDAVRRAADSRVQGVLVGRGTLGAPWFFRE
                     KEQARRAFVEQVDLSTLPATMWEPPVSLRHRMQVMLDHARQYEAIAGLECFRSIRKHL
                     GWYCKAFPHAAAMRGKMFGVSNVQDVERIVAEFCQDLILEEAAGSDAAPIAPQFPYPC
                     AS"
     gene            38918..39550
                     /locus_tag="PK589_01710"
     CDS             38918..39550
                     /locus_tag="PK589_01710"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNJ18337.1"
                     /translation="MRLILASTSPRRHELLTLLGVPFDVVAPPFIEQVQPHLSADSQA
                     VEFAAGKARSCVSSCPDALILGSDTLINLGSEVLGKPLDLAGAEVMLRRLAGQTHRIV
                     TAVALAGPEPERCDVRLAEVLVTMKPLNEATLANYLRTGDSLGKAGAYSIQGGGAAFI
                     ERIEGDFTAAVGLPLQLVAEMLRQRGLSCPVDIDQLYASRPYPNWDRFRP"
     gene            39657..40331
                     /locus_tag="PK589_01715"
     CDS             39657..40331
                     /locus_tag="PK589_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18338.1"
                     /translation="MRVNDGRVGRRGQTHAVVGLVIGAMALAISGPAWAIDVKLSPED
                     AKKALEAGRAPMEKAASPEDVKKILQQASLVTRVGADPEKDPCGASAILRTKRYRLEA
                     FGRQEAAESKKQKKDVRMPDEFIQKVVDMPNMEVEVQLCGDDEYFAEKADIVFQQKGK
                     NIRPVDLSPADRGRKNDGTGPAYRSRFTARFGYESFDPHAKTTIIVTLQDGQTMQFDA
                     DFSQVK"
     gene            40481..41449
                     /gene="pdhA"
                     /locus_tag="PK589_01720"
     CDS             40481..41449
                     /gene="pdhA"
                     /locus_tag="PK589_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNJ18339.1"
                     /translation="MDRADLFSLYRQMLLIRRFEEKSAEMYALAKIAGFLHLYIGEEA
                     IAVGAIAALRPDDYVISAYRDHGHCLARGSDPGKVMAELFGKATGLCQGKGGSMHLVD
                     LPKRFMGGYAIVGGHIPLAAGLAFASKYQKQDLVTVCFFGEGAVPSGQAHEAFNLAAL
                     WKLPVIFICENNRYGMGTPVQRAVALYENVAEAARSYGITAERVDGMDVLAVRGLMCK
                     VVDQVRAGQGPYFIEAMTYRFMGHSMADPSHGHYRSKEEVEKYRKRDPLVVLKETMVS
                     QAQCSEADFKQLERDIADVVAAAVKFADDSPFPDPASLHADILVED"
     gene            41453..42430
                     /locus_tag="PK589_01725"
     CDS             41453..42430
                     /locus_tag="PK589_01725"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HNJ18340.1"
                     /translation="MLLSYREALNQAMREEMRRDSRIFLIGEEVGYYQGAFKVTKGFV
                     EEFGPQRVVDTPITEAGFTGLAIGAAMAGLHPIVELMTMNFGIVALDQIVNNAAKIRY
                     MSGGQLSVPLVIRGPGSAAHQLGAQHSQSLEAWFCHVPGLKVVAPATPHDAKGLLKSA
                     IRDHNPVIFIEAQLLYGTKGEVGDGEYTIPLGQAEVKREGTDVTIVAYSKMLLVALDA
                     ADQLRREGLDVEVIDPRTLKPLDLETIVTSVTKTGRLVIVEEGWRFCGLGAQIAESIY
                     SAAFDYLDAPIVRVTGEDVPIPYSRPLEDAAIPDVPRVIAAVKSLCGAA"
     gene            42494..43693
                     /locus_tag="PK589_01730"
     CDS             42494..43693
                     /locus_tag="PK589_01730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNJ18341.1"
                     /translation="MASRVVMPKLTDTMEEGVLLAWKKHEGDPVHAGEVIAEIETDKA
                     VMDLEAFAPGVLRKVLVHDGETVQSGKLIAVIAEADEDIASALSDGVTAAASTGTGPK
                     SAPSTDAAASVSSGRPRGERVLASPRAKALAAERGVELSTLTGTGPGGRIVEDDVTAA
                     SASVAHALPAGTDQPLSQMRKAIARSTVQSKAPVPHFYLTVDIDMEQAERVRDEFKQS
                     RQPHPSITDVLIKAAALALKRHPEINVSFTGEAIRRYTQIDIGVAVGMDDGLITPVVR
                     NCGGKTLAEISVETKQLIERARHKRLSPQEYTGATFAISNLGMFDVDQFIALLMPPQA
                     ASIAVGAIRDVPVVSRGAVVAGRRMKVTLSCDHRALDGLMGAQFLKEFKRVLEHPEEL
                     LTPVVTP"
     gene            43690..44598
                     /gene="lipA"
                     /locus_tag="PK589_01735"
     CDS             43690..44598
                     /gene="lipA"
                     /locus_tag="PK589_01735"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNJ18342.1"
                     /translation="MSFIQIDSRHTAEPQEPSRSQSRRLPPWFKVRLQTGPDYHDIRN
                     TMDRLKLHTICEEARCPNVWECWNARTATFLILGDICTRRCHYCSVATGRPHAVDHEE
                     PLRVAEAIHALNLRHAVITSVNRDELEDGGASVFAETIRHIRRLIPSCTIEVLIPDFE
                     GNEAALAAVAAERPDILNHNIETVRRLFPSIRPQGKYQRSIELLGRAKQMGMTTKSGL
                     IVGMGESTDEAREVMRDLRSVECDIMTIGQYLQPTKEHLAVARFYHPDEFAALKEEGL
                     ALGFRHVESGPLVRSSYHAEQQVSGR"
     gene            complement(44681..45913)
                     /locus_tag="PK589_01740"
     CDS             complement(44681..45913)
                     /locus_tag="PK589_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ18343.1"
                     /translation="MSFLWLTIQSALRVLRRNPLRAGLTMLGIIIGVGAVVGMVSLGQ
                     GATASVQREIASLGTNVLIIIPGATTTGGVRGGLGSVSTLTVDDARDIEKRVGDIGLV
                     TYASRSVLQAIHEHKNWNTIAIGTTTAFTDLRNWPVANGSFFTQADEDAAAKVVVLGK
                     TVADNLFERGEEIIGAQIRIKNVPLRVIGVLSSKGQSLSGQDQDDIVILPFTTAERKV
                     LGTKFLGTVGIIMVATQHRYSVPTAVEEITELLRVRHRIHPAEENDFTIRTMEDVAKT
                     VAGASRTMMMMLLSIASISLVVGGIGIMNILLVSVTERTKEIGIRMAVGAKRAHILLQ
                     FLVEAIILTAIGGVAGVVFGILGARLLTHLIGWPTIISSQSIAVAFLFSLAVGIFFGL
                     YPANKASRMNPIEALHYE"
     gene            complement(45924..47243)
                     /locus_tag="PK589_01745"
     CDS             complement(45924..47243)
                     /locus_tag="PK589_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ18344.1"
                     /translation="MTSDHSDIDPPRAAHPTPVIVNAVPVVQGQGHGRAPASISQQTL
                     PRHRRRAPIVIGMVGVLTLLAAALWHWWSNDPVNGHYKTVTIDRGPITALVTATGAVN
                     PVISVQVGSQVSGKIAKLYADFNSVVREGQILASIDQKPYQAKVSQAKAALKSAQAGL
                     AKARNMLVQKTLELNRMAALRKEQFVAQADLDLARTNARDAEAQVDVSDAQVDQAKAT
                     LASAELDLGYTTIFSPVNGTVVSRNVEEGQTVVASFQTPTLFVLAQDLTHMQVIANVS
                     ESDIGGVTEGKSADFRVDAYPRESFHGIVTQVRNAPVSIQNVVTYDVIISVDNPDLKL
                     KPGMTANITIVTARNEDALRAPNAALRFRMPGVAVDRKTPQLWVLDASGHVRSVPVSL
                     GIADSLYTDITSSDVHEGESAIVGLATAEDTAQEKLPPGFEFGPKMR"
     gene            47498..48877
                     /locus_tag="PK589_01750"
     CDS             47498..48877
                     /locus_tag="PK589_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ18345.1"
                     /translation="MTEEWGAVLVVDDDADMRSLLCDVLQGRGHQCTGVGGGDEALKE
                     LSEEDYAVVLTDLRMKGMQGTELLAEIKQRYPDIGVILMTAFGTVETAVDAMRHGASD
                     YLTKPVKTEELVRVVERAVREASLRREVSRLRKEVHKEYSFHQILGKSKPMQAVFDLI
                     RRVADSPTNVLITGESGTGKELVAKAIHYNSDRRDAPFVPVNCAAIPEQLLESELFGH
                     MRGSFTDAKMDKRGLFEEAQKGTLFLDEISELPLMLQAKLLRAIQEREIRRVGATKSI
                     PVDVRIIAATNLNLVDEVKNKRFREDFYYRLNVIELRLPPLRERREDIPILVDAFLKK
                     CAAARGRQVKGLSEPALAMLADYAWPGNVRELENVIERAVTLSHGDMIGAEDLPIQVQ
                     GARGDRRILDEAAERTLPLHEVEKEYIVKILEKTGGNKYQAAHVLGIDRKTLYRKLAE
                     IEGKPHSDA"
     gene            48914..51595
                     /locus_tag="PK589_01755"
     CDS             48914..51595
                     /locus_tag="PK589_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ18346.1"
                     /translation="MHPLSQKSLLLEYVGPALLTLGIFLLDLSVSAEYTVWLLYAIPL
                     ALTAANSRPGASLYGAGLVSLLVVIGGVASPTGVLPVSFWVNRFLGIGFMAAISFLFA
                     NRRNHSVPTVPPTMPEPAHKESTPTETVGAIAHAEAAVEGAVAGQRRAEAELHRDKLR
                     FEGIVQSAMDAIITVDESQKIVLFNQAAENMFQWSAEDMLGRPLDRLMPERFRSAHAE
                     HIREFGRSGITTRQMGALGVIMGIRSTGEEFPIEAAISQIGVAGTRYYTVILRDITVR
                     KRLEQELAEREALLRAIIETEPECVKVLALDGTVRTMNAAGLAMIEAASNAEIVGKDI
                     CHLVAVESRSVFRDIVRKAGEGEAGRLEFRIVGLQGTPRWLDTHVVPLRAPDGSITAV
                     LGVTRDVTEWKKAEQLLRQSEERYRRLLGVLPDAVLVVRDNRVFFANEQGLRLFGATR
                     AEDLQGKWIYDLIHRDYHEAVAERFRHLLAGGGAVPEAEERILRLDGAMVDVAVRAAQ
                     FRDEDGAGILVVLRDTTMRNVGQQRLKESEERLQSLLESMEDVIWSSSLDLTTLHYVS
                     PSVGQIYGRAAEEFVARPSLWMEVVHAEDRALAEQALRDLQAIGEYDVEYRILRRDGE
                     VRWVHDRGRIIKDETGHPVRIDGIASDITERKRLQAQLRRTERVAELGTVASGMAHEI
                     GTPMNVILGRAEYLMERSKEESVKKGLQTIISQVERITRVMNQLLAFARRRPVEHRAL
                     DLRQTIEDNLEIFQERLSHSHITVETSFADDCPCVHADADQMSQVLINLVMNAIHAMP
                     EGGTLRVMLAPMPDRGMVTLTVADTGHGMPQEVIAKIFDPFFTTKEFGKGTGLGLTVV
                     RGIIEEHSGTIQVESQPGAGTQFTIRLPIHVEPSRKS"
     gene            51853..52257
                     /locus_tag="PK589_01760"
     CDS             51853..52257
                     /locus_tag="PK589_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ18347.1"
                     /translation="MNETTPAVLLVVEDDKDMRSLLCDELWGEGYQLREASNGVEGLE
                     AVMRAAPDLIVTDLKMPSGGFDYVHRLRDCVPACPIIVMTAFGDARTKEEAMKSGATA
                     YFDKPVRLSELKATVKRLLHRSDSLPEEPSFH"
     gene            52400..53803
                     /locus_tag="PK589_01765"
     CDS             52400..53803
                     /locus_tag="PK589_01765"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ18348.1"
                     /translation="MAGSHAPATPLSPFADPILAARLEALRQLADGLTDRVAVMDRDL
                     NVVYANDAAWGKSGKPPAGVKPAKCYESFVQKQDPCGTCPAESVFVTGEVLTVSCNTS
                     GDGTACGMHQAFPLISSAGEVGSILVLFHKRSDPPAIPAALPQGKLGASRPERTDSRL
                     GDLVGASPAMNQLFEMIRLVADSSATVLIQGESGTGKELVARTIHQTSYRRDKPFVVV
                     DCGALPETLLESELFGHVKGAFTGAAGAKPGLFEEADGGTIFLDEIADTSPTFQAKLL
                     RVLQEGEIKRVGGTQPIKIDVRVISATNKDLTDLVKAKAFRQDLYYRLAVLPVHLPPL
                     RERRADIPLLVTKFIADSCHRHRQELRQVSEEAMRALTAAPWPGNVRELQHYIERAVV
                     TTTHSELTCTDLVSLKFHLEPADLRTVGREAAKQAERARILQALQQTAGNRMKAARVL
                     KISRASLYNKLHDFGIQ"
     gene            54393..59330
                     /locus_tag="PK589_01770"
     CDS             54393..59330
                     /locus_tag="PK589_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18349.1"
                     /translation="MRSSVQSKRRRISRKAMLSLQAALLSGSLLAAPWVFAAGPGDQS
                     HEGHATPVAMPGWTQTLKGQTVVENAMEGRAGNAEKMEMQHHRLMEKLENQAQTDAKA
                     QQTSGAFNEMSMMHQYMGQDGSSFLLASDSSKGEPVMTSGGKCPANAPVKKYDVSMIN
                     IEISLNRWLDFYPGYMYVHTADIDKARAEEAKNKEAREKDGFDPGAVTTGNQGDVIQP
                     LVLRANQGDCVKMTLRNQMEGEDGSLVIQASSMIVSSTGKPATTTNPDTVVSPGKAQE
                     FEWYIHPNMQEGVRQFHSWSHDRELTVLGLFGAFIVEPKGSKYLDPLGTGAAPDTNSG
                     WQVMIDNGSGPDFREFVLFYHEIGDEAFRPLNKKGDFLPQRDPLTDAYRPGGRAINYR
                     SEPFGIDQMHLQHEYFGFEDESLAYSAYTFGDPPTTVARGYLGDPVKWRLVHGGSEVF
                     HSHHPHSGTIRWQRSPRADQEEHWYKGQDGPVKYPVVRTKSDRVDVEVVGPSEALDLE
                     PECGGGGCQHLAGEFLYHCHVAHHYVAGMWGYGRFYNTLQVGNAHTDTMPDMRELPDR
                     LGRMKQGVSSDKLIGTTVDWFGKTFKIVDKSQKTNWKSDPVIVNIKDWVEMFVPAQGQ
                     PGHTNDEKGQIMAYDSTVWDWKWDGNIARGERESTAPNPKYAWAAKWDDSTRPAILFD
                     PTTGKMAWPLFKPHFGKRVPFSANHSGAPWLEPIHQDANGERTSEPALPGEQGRWSLC
                     PDNANRKHYNIHFVRMPITLAKKQGKEPAMVDKDGLIYVLHEEERLTRANDDLKLPAV
                     IRANVYDCVDLLLTSEWDDDDYTNFQSSKINIHPHFFQFDTGNSDGVISGMEYEMSVR
                     PFTMWGKKTKHGLPAPMVAKLTGEAKAGATSIKIQMAPGATAFHVNTELMVGMDCLEK
                     GNDPAASLPRDKSCQEVARVKEIKGDVVTFFKPLKNHHPAGDLVSPEFVRYRWWVDVD
                     MGTVFWHDHAFGATTWPHGGFGVTIVEPYGSTYHDPKNGKLVYSGPVADIHSNEPIGA
                     GVSGSFRELMVSIHDTVPHTVNVIEAGNPPGQPIEVALEAGKTVSFQMPDKILNAPNK
                     YINGGTHTTGSGFNFRAAPFAQRLSNNPDTSKLFSSAIHGDPGTPLLRAYTGDTMVFR
                     LLHQLMNESHVWTIAGHTFLTERYAPDANRKNSIHVGIAERYDLVTKAGGFQGMPGDY
                     IHFNGRTSHFAEGGWGIIRVLDKETADLKPLPRGTNPLGIPATPNSVCPSDAPVKSFN
                     VVALDRPMKLNPKAPDAIEVDFERKIEMTMPEGKIFALEEEAATVAGNVMPNPLTLRA
                     NLGDCIKVNLKNKMKASRASFFAPGLAFDPKDSQGLNVGNNAGDQTVAPGESRTYTYY
                     AHPANKETTSLVWDGGNIVVNPRNGLYGAIVIGPRGSQYRDPVTGADVSQKNTWRADV
                     IVDTTLSENAGKRNYRDVALFFQDEDNIIGTAFMPYVQNVAGLTSVNYRAEPYKFREE
                     QGCSLGKIFQPCAVDKPEDPVTPLIEAHAGDALRIHVIGANSEQNGMFSVEGHEWPIE
                     PYMAGADMISVVEYAGSETLDIFIRGGAGGPYRQVGDFVWSNARLPYTQSGQWGYLRV
                     LPTGDSRIQPLSASGAGARQAEVLPEPQAIPTAMK"
     gene            59531..60481
                     /locus_tag="PK589_01775"
     CDS             59531..60481
                     /locus_tag="PK589_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ18350.1"
                     /translation="MTHRISGWKVGLAAMALGGLLASHPASAYEEITVSDGGTLSGVV
                     KLEGTVPKPKGYNLTTLPDPYYCGRISDGEGWRILQPFNVGPKGEFREVVVYLEGIEK
                     GKSFAEKEVPQIEAKDCLFLPFTTVVRDDQSVTVVNMDPVMHDIQAYETSNLGARVLF
                     NVPLPMNPQHPRNFKDRTEAGMYHKHMAGPPMKELVKLSKGRRIFVMQCGFHAYMESW
                     GVAIANPYFAKTDEEGRFTMTDVPPGTYKLVVWHPYIRTATEKIVTIGPKGTVETTIG
                     VPAPTGRLYANEVLDHAYTRYNVTEEAKKEIDPLLQKQEH"
     gene            60519..61451
                     /locus_tag="PK589_01780"
     CDS             60519..61451
                     /locus_tag="PK589_01780"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNJ18351.1"
                     /translation="MGIWRGGCLRALLGCVAIGLLPGLVTPSHADHELPKPPPLWSPL
                     DNVERLALIDVPNGMAEVPAGPFLMGSDPKFDRAAGPQELPQHQVYVDAFSIDRYEVS
                     NVNYLRYVLATGAAWPHYWREQPFPEKMAKHPVIGVSWREADAYCRWRGARLPTEAEW
                     EKAARGEDGRMFPWGNEPAGWIKSNIAHPGSKRGAKYPPLANVDRYDNGVSPYGVHQL
                     AGNVSEWVSDWFDPEYYRRQENRNPQGPASGQDKVFRGGSWNEDPEVARSAGRNSGGL
                     DHWSYLTGFRCATSGESGRQLSSIDPAMGRNTDR"
     gene            61483..62442
                     /locus_tag="PK589_01785"
     CDS             61483..62442
                     /locus_tag="PK589_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ18352.1"
                     /translation="MKRLQKGKRKRTRLAQALVIGAALFALAPIARALDTQDIVVEWT
                     EEGKKIAQERVANWKTKDEMVLVPAGEFLMGSDKKTDRLAYRSEIPQRSVYLDAFMIG
                     KYEVTALEYLKFVLATDRLPQLDWRYDGGNFQDTMAHHPIMHVSWYDADAYCKWAGKR
                     LPTEAEWEKAARGVDGRLFPWGSEYAGPTRANFGRTGLSGPVRDRPERLLLYPPIISV
                     DKYENALSPYGLYQTIGNVAEWVSDWYDQDYYKTAPARNPKGPETGTQKAFRGGGWMD
                     STTTMRAAMRNGTDPKTKINWMGFRCAQDAKEDTGTKVSMTKE"
     gene            62912..63484
                     /locus_tag="PK589_01790"
     CDS             62912..63484
                     /locus_tag="PK589_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNJ18353.1"
                     /translation="MKRKPCLIEHCDTCGLREKVVLCDISGSDLQEFQKIKRTLEYAP
                     HQTVFYEGHLCLGLYLLCSGKVKLTRSSTRGRRQIVRILGPGELIEKHVFGDRALHEV
                     TCETLEPSQVCVIDKERYLAVIHRNPQLAIKLIQLLSNEVGVNMDHLDQFTFKTARER
                     LAGLLLELGDRFGKKKTIKFESVSPSSVKK"
     gene            63490..63621
                     /locus_tag="PK589_01795"
     CDS             63490..63621
                     /locus_tag="PK589_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ18354.1"
                     /translation="MAGITVETTIRLLGVFRDEGVLTIDGRTITLLNPDRLSRIAER"
     gene            63931..64479
                     /locus_tag="PK589_01800"
     CDS             63931..64479
                     /locus_tag="PK589_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNJ18355.1"
                     /translation="MVPQGSRRTSLLVDPLALLKREHHMILERLAMIETAMSPCCSGS
                     GAVKQTNRDTLRDLLEFFTGPVDVHFKREEMLVDNLRRTLGRNREEKEQFQSFVEEHQ
                     ALKDDATAVMQQLARKRSDGQDGVEPKACGGLRALTDELHRLIRRYREQIACEERLLF
                     ALAEMRLTADQRRRISQRMLQV"
     gene            64722..65423
                     /locus_tag="PK589_01805"
     CDS             64722..65423
                     /locus_tag="PK589_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18356.1"
                     /translation="MKRVRKVVQNIGMWGAGVAIAAVLGVAGVQPASAEEGTGNMVFF
                     KGGFMNLNQDRSGQIFTDTAGIAGANGGNAGWYAGAGLDLVMSKDVWGGMDKTWVLGE
                     IGLQFNSINSSRVNNAAGSIATRTLTDTLATDPQKVQLTMVTIDVAPKIKFMEGSAFR
                     PWVIPVGLDIHVISPPSNRTQYLDLGVQFGAGFEYAVWKAFKVGVDARYHLTARLTNT
                     NNDYFQVGPYVGISF"
     gene            65524..66069
                     /locus_tag="PK589_01810"
     CDS             65524..66069
                     /locus_tag="PK589_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18357.1"
                     /translation="MASFRIEISGGRALVDITLYLPDDSSLPFPIGEHSPQQAFSHRI
                     EETGSGLHHVVTFTGLPEHIDLALQAIGESVRIPGAWAAVGGQRMSSLVALWNRLDCY
                     RQSLSCSDRVSYCRSKAASVRVGLGCGLAFCSAPCQFLCPLCSLKDTPLAQSIVHPET
                     QVLVVLGEIDWCPNLARPADR"
     gene            66143..66616
                     /locus_tag="PK589_01815"
     CDS             66143..66616
                     /locus_tag="PK589_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18358.1"
                     /translation="MFALVWIHLLAAVVWVGGMVFLSVVLVPVLKRDGGFARHAALFR
                     TAAYRFRALVWGAMGILVATGSVLAAGRAIPLSDPSQWPSIFMTKIGLVAFLFTLTLL
                     HDLVVGPQVRRILGTAEAARSAKDRLLVRYSVLVPRVSLLVALLVLLAAVVLART"
     gene            complement(66700..67425)
                     /locus_tag="PK589_01820"
     CDS             complement(66700..67425)
                     /locus_tag="PK589_01820"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNJ18359.1"
                     /translation="MILYETHLTWLRRILAGKGLIIALIVGFLAVESIVAACFLSLMH
                     FKTSNNWATRNEQVLIELERVRSIVSGAETHQRGYLITGSDNYLAPYREALDMLQEQI
                     RRIGSLTRDNNLQQDRVAYLATQVDQRSDEMEQAIALRRTKGLPGAKSIVTQNQLNRT
                     MDTIHDITEQIRDEEARVLARNRADSEAWALTTGSLALVFFLLNAVVFALCGVVMKLA
                     LSSHAQTERLVDALRPSTTPATR"
     gene            67738..68985
                     /locus_tag="PK589_01825"
     CDS             67738..68985
                     /locus_tag="PK589_01825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNJ18360.1"
                     /translation="MARVVIIGASIGGLPAAYEARELLAKKHKVTVISNVESFHFVPS
                     NPWVAVGWRTRKDISFALGPVLEKKGVEYIHATADRIEPEQNRVITSKGEVPYDYLII
                     ATGPKLNFGAVPGLGPSGYTQSVCNVDHAEQAWGTYQAFLKDPGPIVVGAAQGASCFG
                     PAYEMAFILDADLRKKKLRKKVPIYFVTPEPYIGHMGLAGVGASRRLMEDEFAEHAMK
                     PMCNTSIKEVQPGKMVLEGGQEIPFRYSMIIPPFAGVDAVAGTPGLCNPKGFVNIDAY
                     QANPKYKNIYSVGVCVAIPPVEQTPIPTGAPKTGYMIESMVSAAVHNIKADIDNDTKR
                     ETATWNAICLADMGDTGVAFVALPQMAPRNVTWAKKGKWVHLAKIALEKYFLRKMKKG
                     VSEPYYEKVILKAMGIAKLEEPA"
     gene            69006..69803
                     /locus_tag="PK589_01830"
     CDS             69006..69803
                     /locus_tag="PK589_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNJ18361.1"
                     /translation="MIEQALRAVVSGVPIGLQLGATGTGGAILGVPLMVYIAGMSLQQ
                     AAAMSLMIVGASSLVGAWEYGRQGMVKPKAAAAFSWTGMIGSWGGAFVHHMIRQEILL
                     VLFGLLLLLVRTVMARQRELAAEHGADESCATLFPRTCWMKVGGIGLMVGTLNGLFGV
                     GGGFMVVPALILVLGFPARAAIGTSLMIIFMISIGGVVGHMQFGHVEWSLLGYVLLGS
                     VTGMVLGVRADTWVSPVVMRRVTASITISIAGILIVVNLAKLAGWLG"
     gene            70005..70958
                     /locus_tag="PK589_01835"
     CDS             70005..70958
                     /locus_tag="PK589_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18362.1"
                     /translation="MVTPERTAQAEWSAIAEQKTSYTTDAFQFSSARRLRLTEDPSLP
                     TVVSTDKPQDVIWEPALEVIHSTPTMQGKNELSVKAQGAIYTNNPIFNHSDFRIQDRF
                     SLDQATSVLVRYRYIPNLFLGPNFERRTGTRSVQEERVSSHHWRAEVERRLSDTVTAT
                     LITRFGIRRYNDVFAERDTNLWTVGSRVDYRAKEWLTLTLSYLYERGLADGHKETQFA
                     DDVSYYLHMVSAGTEFRLHKEWDLGLTYIHRRKTFTSGIAGDSHVDRFDATHQGIAEL
                     RYHISTAATAMLSFQYGKRTSTNVLRDFQDSIVSIGGQYRF"
     gene            71077..71265
                     /locus_tag="PK589_01840"
     CDS             71077..71265
                     /locus_tag="PK589_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNJ18363.1"
                     /translation="MKLNEMLRLIAGVFVLIAVVLGATVHPYYNYFAAFVAANLIQSS
                     FTGWCPMMALLRRLGVHE"
     gene            71294..72340
                     /locus_tag="PK589_01845"
     CDS             71294..72340
                     /locus_tag="PK589_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ18364.1"
                     /translation="MLALLLAAGCGPSQEPGKPGNMTPGTAGQEPIQVPVIEVAASQV
                     PISVEVTGQVTAVYQATLASRIQGMIDNLLVREGSVVRQGQTLVVLDNRDVQAELSRV
                     TAELDNAKAHQDRMEELYRKDAVSKQELENATRALRVAEAGRKAVLAQLSYTIVKAPF
                     DGVITEKKVERGELASPGQPLLKMEDPGRLRLEATVAESDVRVVSVGSLLPVVIDALG
                     ANPLKGTVSQILPAGDPQTHTFTIKVDLPQAGGLKSGMFGRLKLEKGMSTTLLLPKSA
                     LIERGELASLFVVGGDQTARLRWVRIGRTLEQGVEVLSGVDAGERVIADGSKGRDGAL
                     VQDMTAVALPPQAR"
     gene            72409..75684
                     /locus_tag="PK589_01850"
     CDS             72409..75684
                     /locus_tag="PK589_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ18365.1"
                     /translation="MTTPRLGASGRLAALFVGSKLTPLIILASLLLGLFAVLLTPREE
                     EPQIVVPMADVWLPFPGASAKVVEEQVTKPIERKLSEIKTVEYLYSISRPGGALIIVR
                     FYVGEPMEQSLVDLYDKLMSNQDLLPPGAAPFQVKPRDVNDVPIVTLTLSSERYTDFQ
                     LRQLGDQVLEDAKKVPGTASGFVVGGRGRELRVQIDPTRLKAYGLTPLRVASVIQGEN
                     LALPAGRFESRNESFLVETGRFIRSKDDLDGVVVGVNEQRPVYLRQVAEVVDGPSETN
                     QYVWFGEGARSSSSSSGETPAVTVAIAKQAGTNAVTVAQGVIRKVEEMKGRLIPADVQ
                     VTVTRDYGETADEKANELLWHLLVAVVAVVVFLGLTLGFRPAFVVSIAIPLTLALTLF
                     ISMLIGYSINRVTLFALIFSIGILVDDAIVVVENTYRHLTLRLLPHREASLFAVDEVG
                     NPTILATMTVIAALLPMAFVSGLMGPYMRPIPVNASIAMFVSLLVAFIVIPWFCQTCY
                     RPGVHMAGVDHDSFEEGRSYRLYRRLLAPVLSHPVMAYLVIGVIGLLLAGSVLLFWTR
                     DVVVKMLPFDNKSELQLVIDMPEGTTLEETARATKALTQYVRTIPEMRDYQAYVGTAS
                     PFNFNGLVRHYYLREQPHEADIQINLVAKDRRQAQSHDIAQRIRPPVQDIGRLYGANV
                     KVVEVPPGPPVQSVLVAEIYGPEYDRQIEIARDVRRMFETTAGVVDVDDYLEAEQVKY
                     VFTVDRAKAALAGIPSEEIVRTLRLALEGADVGLVHIPKERRPVQVKLRLPIIERTGL
                     EHLGEIALRTTAGHMVPLSELIRVEQTVREQAIYHKNQKPVVYVIADVGGTGPDKGES
                     PVYGVMGIGKKLEQYRLPEGYRLEQQYAVQPWSEQRFAMKWDGEWHITYETFRDMGLA
                     FAAALVLIYLLIVAQFQSFLTPVVIMAPIPLTLIGILPGHWLTGSYFTATSMIGFIAL
                     AGIIVRNSILLVDFIQHQEAEGIALLEAVIRAGAIRTRPILLTAAALMVGAFVIILDP
                     IFQGLAVSLLFGVGASTVLTLIVIPVLYYLWIGERRPVSACPLPQADNPAAPRHAGGN
                     QPG"
     gene            75900..>76128
                     /locus_tag="PK589_01855"
     CDS             75900..>76128
                     /locus_tag="PK589_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18366.1"
                     /translation="MGSVGHEQEAPLLSWCEADTRRYVQMLVLALVSGWAYFANLHLS
                     LLEGSEGLYAGIAGEMARRGEFFDLTYQGIPY"
     assembly_gap    76129..76228
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <76229..77743
                     /locus_tag="PK589_01860"
     CDS             <76229..77743
                     /locus_tag="PK589_01860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNJ18367.1"
                     /translation="EMARRGEFFDLTYQGIPYFNKPPLFFWLLNLSTSIWGDHEIALR
                     LPGSLAAVGTILLTYVLGTQLMSSRAGFWAALVVATSHVFLWYGRRVLFDSTLTFLVT
                     LALFAWIQVQILGRSSRWYLVTFLSMALGAMLKEMHGFFLPLLIMALYAVIQRDARMV
                     RDRFFWIGLAGAIALLAWYSQLLSVGYQHHFLLGSAIKSVWNSGLSGAAGTSKDGHPL
                     YWYLGMMWADFFPWCALLPSALLLLWQQRPLRAHQTELVLLVWVLGLFTAFSLATLKR
                     EPYLTTVVPGIGLMIGYFYHRSSQSSDGSRTMSPVLRLFLVVLALVFIGGMFFGAAPL
                     RRRWLVSAPVVSPLFIVIMVSLGGALLYAAITSRLQLALRMVGVLAVAYVVLVVNYVF
                     PAIDEAASPRKATEYIKSVAKEAGPPMFLFIPGWPKNEDAVYYLKRDTVVRDLPDEDA
                     VHHAVRTQGPIRVITEESHMVTLQKQAGLVVETLQEFLQPGRKHLYLLSIQPKG"
     gene            78061..78489
                     /locus_tag="PK589_01865"
     CDS             78061..78489
                     /locus_tag="PK589_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18368.1"
                     /translation="MHPIHPMVVHFPIALLLASLLFDLVALRWRGEEFRATSLSLLAL
                     GVLAALVALLTGHLAEEAVEHSGTIPKQAIETHEELAFVVFWLFAGLLGVKMLSYWGW
                     MRERSLLVLFLGVGGSVLLLVASYYGGELVYRYGAGVLPR"
     gene            78486..79667
                     /locus_tag="PK589_01870"
     CDS             78486..79667
                     /locus_tag="PK589_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNJ18369.1"
                     /translation="MKMIGALTRMVVAMAVIFGAAPVSAEKSDGRSPLGERAVLRAGQ
                     VVQGDYFAFGPQVEISGIVNGDLYAAGGEVIVDGVVNGDVIVAGAKVVLSGTISQDAR
                     VAGAHVTVSGTIGRNATLAGADVHLTEKATVRENVIAGGGSVQLSGAIGRDARVGAWH
                     ATFSNEINRDVIVAAESVRLTSKASVGGRLRYWGEVAPSIDEEAVVHGPITRRPLPEG
                     WSTERARQGLVGMRVFAAIASFLSTLLLGLIMLRVWPQLVRQVTGAIRERPAPSVGWG
                     LAALIVTPLVAISFVVTLVVLPAGVMLLALYGVAAYLARIFAITYAGQLFVRRQDDSS
                     SLARPFVVGLLVYSLLTLVPVIGGLVTLFTVMAGFGGLLVAVKQRLAVRQEQDLVQDT
                     A"
     gene            79769..80815
                     /locus_tag="PK589_01875"
     CDS             79769..80815
                     /locus_tag="PK589_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ18370.1"
                     /translation="MSDVGPAERALRVMDWPADGSLQFEGSGIHALDTGGAVVLSRAV
                     RDAQRRGCDVTVEGLRSEHAQLMNLVSVHWDAMMAHVAARPKGTARLQSVIRNLLDRF
                     LRGLGFIGESAMALLRVGSSPTHLRWRSALNSLRLDGVNALPITGLLTFLIGVVIAYQ
                     GAEQLRKFGTNIFIVDLVGISLLREIAPLIAAILIAGRSGSAYAAHIGTMKVTEELDA
                     LRTFGLSPTELLVLPRMLALIVALPLLTVYADVLGVFGGMLIAANQLNVSFTAFLARF
                     EEAVALRHFLIGVGKAPFFAVIIALVGCYQGFQIRGGVDDVGRHTTMSVVQSIFLVII
                     FDALCSIILNWWNL"
     gene            81047..81853
                     /locus_tag="PK589_01880"
     CDS             81047..81853
                     /locus_tag="PK589_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ18371.1"
                     /translation="MSVAVQTENPVIEVSHVSTRFGPAIVHQDVSLIVRKGEVFAIAG
                     GNGCGKSTLLREIVGLLTPTAGRISLLGADSRDLQETDGGSLYRRFGVMFQHGALFSS
                     MTLAENVAVPLREHTSLSARLIRDIVALKIALVGLPPDSGVKFPRELSGGMRRRAALA
                     RAIVMDPELLFLDEPTAGLDPMIAAGFDDLVLDLKRLLGLTVVMVTHDLDSLWRIADR
                     VVVLGEGHVLGMGTMEQLAASEDPAVRNYFHGPRGRAVQLNHSGGESHRD"
     gene            81864..82826
                     /locus_tag="PK589_01885"
     CDS             81864..82826
                     /locus_tag="PK589_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNJ18372.1"
                     /translation="MEPKVNYVVVGAFVLLLGATVLGAVLWLGKTDYRGIYDRYYVYT
                     RESVAGLSVDSTVKYRGVDVGRVKEVILNPDNSEEVRVTLDIVGGTPVKTDTQAVLVT
                     QGLTGLVTLNLTGGSKDAPPLTSVAGQVYPVIQSVPSLYGRLDGTLAKLLSDQGLSNL
                     VANVNGLAQNASLFLDEQNRAQFRQILKDLGEVTKVLAARSSQVDRGAQHAAEAAERT
                     ARLTEHLGVQLPALLDRMGKSAAALQHMTAEVTRTSREVGDMVGTSKPGIEQFSRQTL
                     ADAGLLVSELRQLTATLTRVAQQVERQPNVLVLGRQGQPKGPGE"
     gene            82837..83484
                     /locus_tag="PK589_01890"
     CDS             82837..83484
                     /locus_tag="PK589_01890"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNJ18373.1"
                     /translation="MLRSRVTGWVGLVLAVYTASGCVLPRSEDSAVRTFVLMREETDR
                     QAAPAGAPAGMHGVLVVAVPQAAPGFEQPRIAYLRRPSEVSYYANHVWADSPSRMVLP
                     LLVRSLDTTGLWRTVVPMPTGIKGDYQLDLSGLVVQQEFLQQPSRSRVQVRAHLVDVK
                     AQRVLGARNFEALEPAPSEDAYGGVLAADRAVATVLATMNEWIASCLRGSGRETC"
     gene            83530..84018
                     /locus_tag="PK589_01895"
     CDS             83530..84018
                     /locus_tag="PK589_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18374.1"
                     /translation="MVYGMRTALKVGFSFGLTSGVITTLGLMVGLHAGTHSMPAVLGG
                     ILTIALADAMSDALGIHVAEESKNHGNTHDIWASTAATFVAKFVIATTFAVPVLLMPL
                     DLAIVASLLWGLTLLALLSYALARAQRVPPWKVIGEHVIIGLCVVAMTHYVGEWVRLT
                     FG"
     gene            84048..85322
                     /locus_tag="PK589_01900"
     CDS             84048..85322
                     /locus_tag="PK589_01900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ18375.1"
                     /translation="MDIKTQLPKVGIGRRCGAIEANLGGYRSLMSDDEYEELTALAKS
                     LQGIKICHVNSTAAGGGVAELLARYIPMLQALGVQADWRLIHGEPEFFAITKAFHNAL
                     QGGHYGLGTTEQDAYLKVNERSAQALGNDYDLYIVHDPQPAAIRHFAGSRDAKWIWRC
                     HIDSSAPDKEVSRFLRPLLEEYEAVVFTMSQFVLPQLAARRVAFIAPAIDPLATKNME
                     LPIDLCRRVIADSGVDPARPLLLQVSRFDPWKDPLGVVRAYRLVKEEIPEVQLVLIGA
                     MAGDDPEGWEILDRVEEEAANDPDLFVFTNLAGVGSMEVNAFQHGCDVVIQKSLREGF
                     GLVVSEALWKEKPVVAGNTGGIPMQFPEPFQRNLVDSVEACAERVRHLLHHPGARAEF
                     GRAGREHVRRHFLMPRLVRDELRLIKQVLNAS"
     gene            85319..87022
                     /gene="glgP"
                     /locus_tag="PK589_01905"
     CDS             85319..87022
                     /gene="glgP"
                     /locus_tag="PK589_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNJ18376.1"
                     /translation="MMQQNDKARVAYFSMEIGLNPLMPTYAGGLGVLAGDTIRSAADL
                     GVPMVGVTLLHRHGYFYQRLDRTGRQSEEPVAWAVTDFAEPLDAHVSVEIDGRNVQVR
                     AWRHLVTGMSGHTVPVYLLDTDVPENSSWDRTITDALYGGDAYYRLCQEMVLGFGGFD
                     LLRALGQGDIFRYHLNEGHAALLVLALMESAIGTAPVNGEVPPEVIEKVREQCVFTTH
                     TPVPAGHDQFPADLAHRVLGRRRCELAQACQHQGALNMTLLALRGSRFVNGVAMRHGE
                     VSHSLYPGYPIHSITNGVHAVAWAAPSFQNLYDRHLPDWRYDHLSLRYAISIPSSEIW
                     AAHREAKLALVDEVNRETNAGFDRDVLTIGFARRATAYKRATLVFQDIARLQRLMKAK
                     GQIQFVFAGKAHPQDEAGKDTIQRIHGMRGQLPVAYLANYDMTLACLLCAGVDVWLNT
                     PLPPMEASGTSGMKAAMNGVPSLSVLDGWWVEGHIEGVTGWSIGDKVEACLAPGSDME
                     ACHAAALYDKLEQQVLPCFYQDRERFIDIMKHTIAINGAFFNTQRMVQQYLRNAYRLV
                     G"
     gene            87046..>87570
                     /locus_tag="PK589_01910"
     CDS             87046..>87570
                     /locus_tag="PK589_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18377.1"
                     /translation="MAQPHDSMQSDYSHLLTLWTAGVRDYHTMLSDYLTANSIFVAVI
                     GLLVSRESLAVPFTLVIVLLSAIGILMSVQMAIVLGRFSGQNALWEWQLRGIESMPDW
                     RERKPVSMLYRLREHRETIVDETNEPRSFEPSWAFRQHRQWWAHRAISFPWFFGTVYG
                     LFLLWAVTQMARSSM"
BASE COUNT        17784 a        23868 c        27090 g        18728 t
ORIGIN      
        1 gcgcttaaac agccccatct gcatcctcct tctggatgag gcctgatttc ggtctagttt
       61 ccccgtgacg gggagtatag acctcgcatt tcgccgcttc aatgagatgg tctaggtcgc
      121 gggtatcgtc gcgagtcggc agtggaaacg gcttgccggg actggtttca aagcttttca
      181 gccacgcctg aatcgcctca ggttcaaatc tgacgaggcc atgaatgcga cggcacggga
      241 ttttgttctt ggaaacccag agatagagtg tggattcttt gatattgagc caagcggaga
      301 gctctttgac ggtgagcata tgcagataca ccgggggagt cttgcgacac ccccaggtcc
      361 ccctaacatg ggctgcccgc gtgaccgccg gcagaccggt gaaaccgagt gggtggttgc
      421 ttgagcaaat tgcggtgtcg gtctttccac cgagcaatgc ccctgacaat ttcgttcagt
      481 agccattctt cccctccagg ggtggcacaa atgaccgcca acatcggcgt cagggtgtca
      541 ctcacccatc gtttcacgtt ctgcagggtc tgcacctgct tctcctgggc caatcgcccc
      601 ttctgaaatc cctccgtcag gagggcgtac cactccaaca ctggagcccg gtagcgttct
      661 tcatcctcag tctctttggt gatgtctcga aagtccacat aggaccggag taagccgatg
      721 accaactcct tccaatcggc ttcgtctaag gtggccaatg ctcgggcaca ctgttccgcc
      781 cgatccttct tcagttccaa ttcccatcgc gtcccgtagt cctgccagtt ctcttgtcct
      841 ttgctctgaa gttcgagccg cttgtcatag atccgcaaca aagtctgact ctgaggactg
      901 ccgaaataca tcgtctcacc cgtcgtcgct ccggtcccat gcgtcaggtt ggagacgata
      961 tgccggacct gagcggccct tgtgacgcat tggcctgctg ataccgcttc tcgaatggtc
     1021 gagacgggca ccgctcccgc tcggtcatcc aacgcacagt cgatgcgcgt gacatgtcct
     1081 tgctgcttat gcacccactt gagcagggtt cggatctggt ccagtgtcag ggcggacgcc
     1141 aggccgcccg agagatccac atggatttca ttcgggcgac gaggggcatt ggtgcccagt
     1201 ttgccgaccc cgcgcagacc gtcagcccgc atccaggaca agggatagcc tcggaaccca
     1261 cctttggcct tactccagtc tccgccaagc accttcatgg tttcttgggg gttactggct
     1321 aagaccgtaa aggccaacca atcaatggtg agggtgaagc tagaatccat ggaatctatc
     1381 gaactcctgt acggagcgct gtgggtagcg cccccgtgtt accaagacgg gggccattcc
     1441 gctccgcccc gcagcctgcc ggcatgcggc gcggaccggc tttgcctttt gatggtgaga
     1501 cggatagcac tcttctcagc gtccttcctg agtcgttcga gtcagtttct gccggaccac
     1561 cgcaaaggcc ttctctccga cctctctggc tacaatcttg atggcttcgg ctttcccatc
     1621 catcaccata ctcatcagga ccgccccgcg cacatacttg gagacggttg tgccttcgtc
     1681 tgcagcgcat ttttcgatta attcgcgttc ctgttccgtg actttaaatt gaagcgtttc
     1741 ggttttggtg ttgtccgtct tcattagtgc ctccagtaat atgcaatata taatatagta
     1801 tatatgagtg tcaattgtgc cgggcaggct ggagttttag cattgcacgg atgatgcaaa
     1861 accgatagcc tttggtctta atacgaagga gagacgttaa gatgagggac ttatttccag
     1921 ggtattacct gccgagtgaa gttgagttta aaaaactttg gagagaaggc ctgttcattt
     1981 tggacacaaa cgtgttgcta aatctatatc gcttccctga caaggcgcgt gctgattttt
     2041 tgaatgtatt acgacacatc gcacctagat tatggatacc atttcaagtt gccttagaat
     2101 accagcggaa taggctctcc gttatgtccg agcagcgcaa agcttatgat gaagtagaag
     2161 atataatatc aaaatcgttg aagcagctta gagataagtt gagcacgcta aacttgccaa
     2221 cgcggcatcc ctcaataaac ccaacgcaat tccttcagga agtaaacgtt cttttcgaaa
     2281 ggtatgcaga agaattgaga acacttaagc agaaagaacg agagcttact tctgaggatc
     2341 ctataagaga tgccatacac gaactattgg gaaagaatgt gggttctaga ccatctaacc
     2401 aagaagagct cgatgccatt ttcaaagagg gggaggatag atatcaacga cgcatccctc
     2461 cgggacatct tgatgccgat aaaagtatgg ataaaggcgg gaataggttc gtatgtggag
     2521 aactcgttta tcagaaagag tacggagact taattctatg gaaacagata attaattatg
     2581 ctcggaagga aggaaaaaaa tacatagttt atgtaacaga cgatagcacg gatgattggt
     2641 ggcacattgt cgataagaaa accatagggc ctagaccgga acttgtagaa gagatcaagc
     2701 agaaaggtgg agtggaattg ttttacatgt acgggactga acgttttatg aaattcgcgc
     2761 ggacgtatct gcaagtctca gttccccctg aatccatttc ccaggtttca gcgacagttc
     2821 atgatgaaca gaggaagaga gccattcagg attttgcaga gaaactattt ctgcctcagc
     2881 ccaactatga agacgtggtt cgtaattggc tccgtgccag acatccaggc gataggatgt
     2941 cagttggatt ggtaccggga gaccccgaca tagtcttcac cacacgcgat gggcagacca
     3001 ttgcctacga agtgaaagta ctcacagcaa taggccctaa ggaagtcgag aggttaataa
     3061 gtagtatggg tggcatgaaa tatctgcata acgaagttca tattgtcata gtagccaggt
     3121 caacccaggc taaggcagca atagacaaaa tagaagattg gattaagaaa gcgagccgat
     3181 tttatcgtag tcttcctgac agtctaatcg cgggtaccgt tgagaatttt ggcgaaggtt
     3241 atgaattctt ccctgtcacc attcttatgg caggttccat tggtgattga ctctgaatag
     3301 ctctcaggca agccaatcct gattcgacag ggctaccaaa gcgaggcggg gtttttccct
     3361 tatagaactg accaagtata caaatggatg ggtttgctgt gatccgttaa gctgcattct
     3421 tcgcaggaag gtactcactc tgagcttcaa taaacagcga tatgcatgtg agtgattagt
     3481 tatgagggag ggattcagat cgcagttctc gattcaccca accttgacgg attaagacat
     3541 tggcagcaat tccgatctga cgttccgtga agttccgctt gcgctcgttc catgcatagg
     3601 tccgttctac aacatcttcg agcgagtgga ccgactcatt tctcatgatc caatacacgc
     3661 ttgctaaaag ttccaagccg aagggggatt cgaagccttt caccaaggct gccacacgat
     3721 cgaaacgact tctggtttcg ggatgattag cgagatactc agtggcatcc tcaaccgctc
     3781 caggaaccaa cgtgagctgc ttgtcaggta cgtcaccgcc gtcatcatag ccagagatgt
     3841 gatgaccctc gattgcatgg agaacatgtc ggaggttctc tgcgtatggc ccataaatgg
     3901 ctcgtctata ctttaatagg aggggctcgc ctgcctcttg catgaagtac attagcttat
     3961 gtacttcgag taaagtaacg taagggtcaa gtagtccatc cagatagcgc tgcactagct
     4021 cgatcaatgt cgcacgactt gcagtcatct tagggacttc gcgccgatgt tgcatcgtgt
     4081 cagaagaagg tgcgccttga ggttcataga cagtgacatt aacgtcgaca aggggactca
     4141 gggctgattg aatctgtgac cgaacttcgt cccaattaag gccgcccaac ccgctaccta
     4201 agggtggaag cgagattgaa cgaatctttt tggcgcgaat tactgcgaca aggtccacaa
     4261 gccctgcctc aatatcctca atacggctct tgccacgcca gtgacgtttg gttgggaagt
     4321 taataatata gcgaggtgga gttagctgac ctgtctcgaa cacaaacatt ctgccaggct
     4381 taacttggcc gagtttacaa gcaactgtat atgctttgaa gttttccgga taggcgttct
     4441 tgaactggag tgcgatacca cgacccatta tcccaacgca gtttaccgta ttcacaagag
     4501 cttcagattc gctccttagg atgtcgccgc gagtgaattg aatcatggca gagaactccc
     4561 cttagtagta ccattcaggg tggatttcga ccgatggtcg atgaccgctc ggcgacaatg
     4621 cgaccaccac ttgattgtag gtcgatcgcg actgaacgcc aatgcgctcc accaaatgcc
     4681 acgggaaact atgttcggtc aagaattctg cctgctttcc atctttacag cttctccact
     4741 ctgttgcctc cacagcattc cagttgactt catggaggcg atttaagtcg cagcgatctt
     4801 cgaagaagta actgcctgca ttggacaatg tgaaggccca gcgatgccta tttcgatccg
     4861 cccaagcaac tgcggaacgt agatctgctt caaggtgaac gattggccct tgtccccctc
     4921 ggtaattgag atctggatgg ttggattgat tgattaaata cagcatgacg gaacgcgggc
     4981 agaagtaaaa gggaacgcag tcgcccacgt aaaggttggg atggctgctc aagcgaattt
     5041 cgttgagtcg ccgctgcttg attccattca tgccgatggt ggtccctaca ggttctctac
     5101 gctgaacttc tctgtcacac cagaggaatt gatccgcaag aattgagggc aatcgatcca
     5161 cgtgtgcaat gtgatagatt ttgggctgtg ctggcacagt cactgagagg tccctcaatt
     5221 tgctgagtga cttgagagga attggaagtg gccggttgtt tcatctgcgg acctacgtca
     5281 attttcttga cgggttgcca caccaatgtt gggaccatgc cttctgcaga taggtcatga
     5341 ttgtgcggga ggataccggt ttctatacat ggaaacaagt ctggtagagg cttggtactt
     5401 ccgtcccttt ccgattcgtg gggtatgctg cctcactacc tccagctcta cgcaataacc
     5461 aaattgggac atgtcagatc gttgtgctga atttgtgctg aaggatctca aaatgttcag
     5521 taatggttcg aattagtaga agccgtagaa tcctgttaac tgctccataa ataagtaaaa
     5581 acggggaaac cttcgcaaaa tcaagccggt gtaaaaatta cttagtatcg actctcaagg
     5641 ctcagacacg ggttcaaatc ccgttgggac caccaacctt ttcacgcagt tagcacaagc
     5701 cggctcaatg gcccttggcc cgacctcctt tccctcaaag acttgttccc ttcgtcttct
     5761 acaaccatcc agcctccagt gactacgtcg gttgagatat cacgacgtgt ctcacttccg
     5821 gcgtaggcac gtatttcagg cattgatagc tccataacta tcaatggaag gagaagcagt
     5881 aggcgcgaag tctcatcata agatgctcct tcgtgtgcca taaaactgtt ttctatttgc
     5941 ggggccctca gtcaggcggc tgcttgtcgc gtcaccgaga ggcgtaccca tggaattttc
     6001 gacagtcccc acgtgaagag gacagataac ccgtacacta gcaacgtgta taggacatgc
     6061 cagatgatcg gcagcgccgg caactgatcg tggaggggga ccagtgtgta tccaaccaat
     6121 atatgagaca catagactcc gagggtgaag cgtgccagga aagggagaat cgtgttctcc
     6181 ccaattgttc gatatgcgag cgtcaataag aagatcccta cgaccaggag caccgccccg
     6241 gcatagggat agtccatgac cagatgaggc gaggcaagga agacccgttt cagcgccacc
     6301 ccttccagca tagccacgac gaatcctcca gcgatcagcg cgagcgcgaa tgatccagtg
     6361 aaggcggcct gttgagagat ccaccatcca aggagggtga agaaaacgcc cagcagtaac
     6421 aggctcggtg aatactgtgg aagcgaaacg actgcactca cgacgagcag gatataggtc
     6481 gagacaagca gagcccccac ccatgttttg agacggctgg tcacgaccat tgcgaccgta
     6541 ctcaccccag cgatcagcgc cggcaagaac cagaggtgaa agatcggagg tctcggcacg
     6601 agaagtagtg tgacatgctc ctgcatgagc ctgcccatgg tatgggaggc ctccgtcgaa
     6661 atggtcgacc agagcccttg tgtgtagacg ttgctgatcc agttcggtcc gatgatcgag
     6721 tacacagcga cccatatgac aaagaggagc gtcagcgagc gacacgagcg ccgtagtagg
     6781 gagaaggggg ctgctccggc atccacggtg cgcccgtaaa agtatcccgc cgtcaaaaag
     6841 aaatagggga cggagatcca gaacaataga tagagcggca tgtccacgag gaagccaacc
     6901 ccgccgccga tgagatgcag acgtgcaatc aggtttgtat ggagggatac gataagcagg
     6961 atcgccacaa cgcggtagct ttcgatgctg gcgatgcgag gaggccgggc atgtggcggg
     7021 atcggtgcag tgcttggcat gaagcgatag tcggaacgat atttctgccc ggtattcgat
     7081 gcaagacaaa cgcctgtcct tgagcattcc tgtcgcacgc acgatgcacc gacagaaccg
     7141 gtgaaatccc gccatccatg aagcgtcgct ctagcgagcg cctcacagat agcagctcag
     7201 atccaattca acggaataag gagtctggtc cgcactgagc gattccgcac cagccgcacg
     7261 atacgaaacg cgttgttcgc ctttcttgtc agtgaaagtc tcacgcagat acttgagcga
     7321 tcagcacccc cgccggtttc ttcttcctgc acgaaaaaac cggcgcttca attgacaggg
     7381 tgaaaaagac agctaaggta gaaatcgcgc agagaaagcg gctaaccgtt tttgcccgta
     7441 gcacagacat cgaagaacag aaggggggag ctcatgaaga aacaaggaat cctcgtgctt
     7501 gcaggtctgt tgattggtct tcaatccggc acagccttcg cggtgaatcc agacaacggg
     7561 cctggatgcg gattggggaa gttggcttgg tctgattaca agacgccgaa aaatattgcg
     7621 ccccaagtca tgatggcgac gaccaacggg acgttcggca gccaaacgtt cggcatcagt
     7681 tttggcacct cgggttgcac caatgacgga aaagtgatgg catcgcagga gacgaatgtc
     7741 tttgtcgcca gcaccttcga cacgctgtcg accgacatgg cgaggggtgg tggagaacac
     7801 ctgaccgcac tcgcaacctt gatgaacgtg ccggtcgaac agcagccggc cttttttcaa
     7861 ttgacgcagc agcagtatct cgcgctgacc gaggctggag aggtgtcttc agtggcggtc
     7921 gtgaaggcgt tgcaggaggc gatgaccgga catccgaccc tggcccagct cgccggcaca
     7981 cgttaatcgg ttcgctcctt ccgcttttcg gaaacggtag tgaacggtcg ggggcggtga
     8041 ctttctgtgc cgccctcgcc ggtcgttctg cctacggtac tcatctatcg ccgttgagct
     8101 gcccctgagc gagagcgaat tccctccatc tgattgtgtc tttgcttgaa gccttccggc
     8161 tgaggggagg ataatcgctc atccatgact cagccttccc accgactgct tctgctcacg
     8221 attatcatcc tggggattgg tctctatacc cttgatctct atctcccgtt gggaatcgga
     8281 aacggggtgc tctatggcgg ccttgtggtc atgtcgctgg caattcccga tcgcaagatt
     8341 ccgctactgg tggccggcac ctgttcggtc ttcgccctgt cggatgtgtt tctcggtgtc
     8401 acgcttccca acgtgccgct ctggatggga ctgagtaatc gcatgttcag cctcacggcg
     8461 atctggtttc cggtcgtcta tgcctttcaa cggcgaaaga gtgaagaggc gctccgcaac
     8521 gcgcacaatg aactggaaat acgagtggaa gcgcgtacgc gggagctggc actggtcaat
     8581 caagcgctcg tgggggaaat cgaggagcga atggaaacag agcggtctct tcgtgccagc
     8641 gaatcttcct tgaaggtgag tcaggaggag ctgcgacaaa gccgtgaaga actgcgggcg
     8701 ttggcggggc aattgctgac ggcgcaagag gtcgaacggc gccgcattgc gcgtgacttg
     8761 catgatgatg tcaaccagcg cctggccatg ttagcgatgg atctcaggcg gatcgaaaag
     8821 ggtgaagcgg gggatctttc gaatatcggt gggatggtaa aatccatcag cggtcggttg
     8881 actgccgtgt cggatgatgt tcggcagatg gcgtaccggt ttcatccctc cattttagac
     8941 gatctgggcc ttatgaaagc ggttcggcga ttggtcgatg atttttctgc cagcacggga
     9001 atcgaagcgg tgtatgtcta ccaggattgt ttgagtcccg tgccaaccga tctggccacg
     9061 tgcgtgtatc ggattgctca ggagagcctg aacaatgtgg cccgacatgc taaggcgacc
     9121 gaggtcgaag tggaattgat ttgtgacgag gggatgatta ctctttcggt ccgggacaac
     9181 ggagtcgggt ttgatgccgt gcatgcgtcg cagggacggg ggcgcctggg attgttgagc
     9241 atgaaggagc gcgtgcggct cgtgcgagga accttgaatg tctcgtcgtc gccggggggc
     9301 gggacgcata ttgaagttgg cgtgccgctg tcagggagtc agcatgtcta aaccgcgcgt
     9361 cttgctcgcc gatgatcatt cgctggtact cgaaggattt cgccgcattc tcgccgacca
     9421 gtgtgaactg atcgggatgg tcgaggacgg ccgcgcactg cttgaggcgg cggctcggct
     9481 cgagcccgac attgtcattc tcgatgtgtc catgcccctc ttaaacggca tcgacgcggc
     9541 cacgcagctc aagaaaacgc atccgtcgat gaaggtgatt tttgtcacga tgcattcgga
     9601 cgccgactat gttcgatccg cctttgaggc gggggcgtcg gggtatctcc tgaagcggtc
     9661 ggcggtcgat gaattggagc aggccattcg ggctgtgtgg gccggacata gctacatcac
     9721 gccgttgatt gcgaaagatc tcctcgatgt gttgctcacc gcaggtccgg gacagacgcc
     9781 acagaaaaaa acgctcacct ttcgccagcg tgaggtgctc cagttattgg cggaagggcg
     9841 aacggtgaaa gacatcgcgc tccgcctcaa gatttccacg cgcacggtcg agtttcacaa
     9901 agcccaagtt atggagcagc tgaatctgcg gaccacagcc gaattgatca aatatgcgtt
     9961 gacccacggt ttgctggctc cttcctgatg taactgctac cgtgtgtctc tccgcattgt
    10021 gcgccgaagc tgacctcgca tcacgtgccc ctggttttct catcaaaatt tcatgctgtc
    10081 ttcagtcggc cttcatcttt gggccgtacc atcccgtctc tcgccagaag cgtcggtttc
    10141 cgggaatgcc tattcgggca gcttgcgctg tgcacgcatc gcacggatcg atttgggttc
    10201 aaccggtttg gcgaccgaga aagggaacag ttatgcgcat tgctcagatc agtcagtgtt
    10261 tcgaggaagt gtcgaacgag agtgagagac cagaggtcag gggcgtggag ttcgtgacga
    10321 ggagcctcat gcggtttggc cacgacgtga cggtcttcgc cagtggcgac agtcgagtat
    10381 caggtagatt aataccggtg tcgcctcaag cgattcggca ctatccgtcg cccaagcggc
    10441 atctctccga aggattgacc tttctggcta tcgaaaaggc gttttccatg gagtcttcct
    10501 ttgaccttct ccacgtccat gcgggctttg tcgcctttcc gatgatgcgc cgcagtcctc
    10561 ttcccagcgt ggcgacggtc tatggctcgc tggatgcacc agaaattttg caattgttca
    10621 gagcgtttcg tgaattgccc ttggtggcaa cctccgccga gcaaattcga cagtgtccgg
    10681 agctcaattg gctagctctt attcccgttc ccggcccccg tcagcgtagc gaagtccctg
    10741 cggaaacaat ggactcactg gtcgaaacgg cgacagccta tacggccgtc tatgaaagca
    10801 tcgtcgagag agcggtccgg cctactcatg cagtgagacc ggcctattct ctttatgacg
    10861 tgcagactac cgtctgacgt agggggggta tgtccaagcc gacaactcat gatgaaccgc
    10921 agaatggcgt catgggcatg gtgcctgtgg cgccgggcag ttcctccgtc aatgacgaac
    10981 agctgctccg cgcttcggct cttgtcaccc gatgtccagt attgaacgct cttctctcca
    11041 ggccgtggac gagcctcacg ccttcagtca atcagacgca gcatctgtaa ctgttcgtac
    11101 ctcgtaatct tctcatacaa actatcgggc tttacgagtt ccgccttcca gcagggtgcc
    11161 gtacaactcg tgtctgggcg tggcacgcca tccgaagatg gccagacatg tctattggca
    11221 ggaatggtgg ccatgaaccg gaacgatttt ctggtggcat tattcgggcg ccctgtctgg
    11281 ttccactgca agcaatgcca tcgggtgatg gctggaggaa cggaggagtc ttttcccgac
    11341 atgtggcagg aggaagcggt ggccatggaa gcctggcgtg tgaggcctga agacctgtgg
    11401 tggtttgata ccgtttgtgg gtcctgccaa gagcagcaaa ggagctaaat cctgttcgac
    11461 ggaagtctcg ctcagacggc aatagagaat gtgggcccag agctggcata tgaacggcga
    11521 actttgcggg ctcttcgcca gtttcggatc ggtgtacatc gcatcatgcc gacggggctc
    11581 ccgccaggat tacttcgggt catcgatcag atcgtgtgtg tcgcgggaac ctccggtcgg
    11641 ggaaccgatc tctgggatgg cattcgtgtc gaatgcgagc gggaatactc cggcatccgt
    11701 cacgggaagg gcacgttggc gcatgcgctc gaatgggaaa tactgaagct ccaaacacgg
    11761 ttggaaagca gtgcctatgg caatccggtc gttccagtac ctctctttcg caatcggcac
    11821 gtgcacatcg ggtcgctgat ccggctgtgg cgacggcttg ccttggagac agaagaatgg
    11881 ctcgcaagtc aggggtatga cacgttgctc gacgtagggc cctggggcgg attcaatttc
    11941 gtgcttgaac cggacggtta cacacggatg ccctttgcgc gactcacttt agctgtgggt
    12001 gggctgccgt ccacgccgtt tcatgaacag ggtgggccgc tgttccagca tctgcttcca
    12061 cggtatcatg ccgaattaca gcaggccggc gtgaactttc cccctctctg gcagtatcac
    12121 cacgccaagc atgatgcctc cgtacgcttg cgggaagtct ctgccacaca ttatctgccc
    12181 acgcatagtt acgaacgccg aacgttcgtc aaagtgcggg tctcgcgaga ttgcgaggcc
    12241 gtcgaggaga tcgcgttgca ggattttctt cttctcctcg agcgcctaca gttcaccacg
    12301 gattgggagc tctatcggga acagacgaag gatgtggatg ctcgcttcga tcttcaggac
    12361 tttatctcgc tcaaccatat ggtcgagggc gtgtatcaac ggaccgatgc ggaggaccga
    12421 cttcttcacg agatgaagga cgcgtttcgc ggagccatcc gcgccagaca ggtgttatac
    12481 ggttatctga gccaggtcgt gaaattgcgt tgggtagaga atctatattg ggcgctggct
    12541 gaggcggctc ttggcgtcaa gcgctaccag cgcatggtct cgttcgatcg cgaggtctgt
    12601 cctcagattc cgccacgatt actcatcccc gtgcggaggc acctgaaccg ctaccacaac
    12661 cgtgtcggcg gaacggcctg agtccaattc aacagactgg ctacagtttg gttgcttcca
    12721 aggtgccgat gctaccggtc accttatgtc ggcggcaacg accgaagcct gcctgcctca
    12781 accacgcttc gacctcattc cagctatagc accggcctgt tccagtaaat gcaaacatga
    12841 gaagtgaaaa gacggccgcc tcagcaggac gggtctcctc tctcgcgaga aaatactcca
    12901 caatgaacag tctgccgcca ggccgcagcg cctttcgggt acggcgaagc aagtgccggt
    12961 tctccgtctt attaaagtcg tggagcacgt tggcgagcag gactacgtca aggtcagtgg
    13021 gaagcggcag ggtccgaaga tcgccctctt gaacctgaat gcggttcgcc agtcgctctc
    13081 gacggatcag gcgtttggcg acggtgacgg actggtctat gacgagaccc tgaagtgtgg
    13141 gataccgccg ggctagtgcg atcgcatagg atcctgctcc gccccccaga tccagaaatc
    13201 tttcggcctg tcgaggcagt cgaaatgagt caatggtcgg ttggaccagt atgtggctac
    13261 gctcctgcat cgcgagtgca aaacggcgcc ggaaattagc ggtcttggtg acgggggccc
    13321 atccaggcgg gccacgtcgg attgccttgg tcatgccgct ccaatccacc caatggtgat
    13381 tgaggagcca cagtaggttg gtgatcgcga gagggctccc gccatctaga aacgtccgac
    13441 ccagcggtgt attccagtag cgcccggctt tcaaatgaag cagtcctagc cctgtcagtg
    13501 attgcagtaa tagcttcatg ccctcacgca ccgttccgca cgatacggcg agttcctcag
    13561 cgctccgtcc gcccgtgccg atggccgtga atacgccaag ttcattggca acgaggagtg
    13621 ttttggattc ggcgtatccg ttcgccaggc tcatgagttg ttggtaagtc atagagatcg
    13681 ccccaggaag cacaaacgac cgcgttttcc caaccggcag gagtcactgt accaccgagg
    13741 ataagtgagt cgcgaatgat tagcgccagc gtttcgggga gtcaccgaga agcgaggttc
    13801 tcaccttcga cctttcatcc ggagccctga ttctgataga agcgtgccat gcttgatgtc
    13861 attggtctcg gtgcggtcgc gggcctcatt cccgtttatc tcgggatttt cgctgccctt
    13921 tatctgggaa aggtgttgcc acgaacctgg gagggtgggc tgatcggggt cgcgaacggg
    13981 gtgctggtct atctcttctt cgacctcatg cacgaagcga ccgagcagac gggcgcgcgt
    14041 gatcccctct catggctggt gtttctgggc agccttggtg tgagtttcgt cggcttagtg
    14101 gctctggaat ccagccaggt gttcggcgcc cgttccggca accggatcct gtcgttgcct
    14161 tacatgattg cggtgggcat gggcttgcac aatctcgggg aaggtctcgc gatcggcgcc
    14221 agttacgccg gtggtgagta cacgctgagc gccttgctgg tggcggggtt cggcctgcac
    14281 aacggaaccg agggattcgg catcgtcgga gccgctggaa aaacgccgat ctcctggcgc
    14341 gatgtggcgt tgctcggcct gattgccggg ttgcccacct gcgtggggac gttcctgagc
    14401 gggcagggcg tgtcgtccta cttctcgatc tgcttctatg cgctcgccgc agggtcgctg
    14461 ctctatgtgg tgctctcgct cacggtgatg tcgtacacgg cgacgcgtcg ggtacaagtg
    14521 tcggcaggca tgtttaccgg catcgcgatc atgtatctga cagccatgat attgacgctg
    14581 gtcagcggcg tacgcagctg acgggtggca tccgacagtg tgactcatcc tgcctcgtct
    14641 ttcttcctac acgtatccgg atggttcgac ttcttgctcc ctcactgcgt gtcacgcgac
    14701 aataggtgag tcgcaacggg gcctctggtc ggcgcttcaa ttgaccccgc gacagtcggt
    14761 tgtgtataaa atggtctgct cgatgaaaga gcagattttt tcgggaggcg ttcatcatgg
    14821 cgggacggga gtttcacgac ggagccaagg atggcttgga ggcactagag cctctagatc
    14881 ccgagaagat tcattctttt acggaattgt tggaagctat gcggaagacg gcgttcggcg
    14941 gccgccgact cggcgaggcc tatgaaacgc tcgccgcgat gatcgaggac cctgactgta
    15001 aagtcgtctt gaccctctcg ggcgcgatga cgattgccaa gatggggaag atcattagca
    15061 cgatgattga ccgcggcatg gtgcaagcca ttgtctcaac cggcgcattg gtggcgcacg
    15121 ggttgagtga atcggtgggg aaactgcact accgtcatga accatcccaa actgacgaag
    15181 agttgtttca aaagggctac aaccgggtct acgacacgct cgagatggag tcgaatctga
    15241 actatgtcga gcatgtggtg tcgcagacca tgaagcgcct caatgtcgac caaccgctgt
    15301 cgtcaaactt tttgactcgc gaattgggta agacgctggc cgaggagttt gaaggtccgg
    15361 gaattctcaa gagcgcctac ctcaagaacg tgccggtgta tattcccgcg tttaccgatt
    15421 ctgaaatggg cttggacttg gggacctggg ccatgggtaa gcagatcgat tttacccgta
    15481 gccagatcaa agagggcggg gatgtggcgg tattgcgtgc gctgcatcag acctgtccgg
    15541 tgtttaatcc ctatctcgac ctgaatcact atgcagaaga agttctaggt tccacgcggc
    15601 tcgggatttt cacgattggc ggcggcgtgc ctcgcaattg ggcacagcaa gtggctccct
    15661 atattgaaat cagcaatacg cgtctcggtc tcaatgtcga gcctccgcgt ttccactatg
    15721 gcgtgcggat ctgccctgaa ccggactatt gggggggctt aagcggatgc acctatcaag
    15781 agggcatctc atggggcaag ttcgtgccgc cggccgaagg ggggcggttt gccgaagtgc
    15841 tcagtgacgc cacgctggtg tggccgctgc tcatggtggg attattggaa cggttcgccg
    15901 cgaagagtgt ctccgcatga cgcccctgac aggttggatg atcagattcg ccacgccctt
    15961 cctgctgctc gtgctcacga cggcctcggc cttggcggcg agctcgccgg cggtcgataa
    16021 tcggatgcgg gggaaggcgg atgcgccgat caccctgatc gagtattcgg acttcacctg
    16081 cgggtactgt ttgaaatttt tcaaggaaac ctggccgaag attcacgcgc gctacgtcga
    16141 aacggggaag gtgcggtttc tctacaaaga ttatcctcgt gccgaccagg gccccggcgt
    16201 cactgccgct ctggccgccc gttgcgcggg agatcagggc acctactggc ctatgcatga
    16261 tcgtctcttc gccgctgatg gccggttgga tgtggatagt tatttccagc acgccaaagc
    16321 catcggcctc gaccaatcga agtttcggga gtgcctgcgc gatgctccgc atatgaaggc
    16381 gattttccaa gatcgggacg aggccaatgc ctggggattc cacggcacgc ccggcttcat
    16441 cttgatgcgg acgacgcaac agccgacggc ccagaatccg gcgctcgcga ttcccggagc
    16501 ctttcccttt gccgcatttg aagaagagat cgagaaactg ctcgcggcct cgggagagaa
    16561 ggcgaagtaa cgccgctcat gggcgtgaac gacagtaggt gaatatcgtg acgagcatca
    16621 tcgtctagcc acgaaagagg aaactgcata tggggtctaa acaagctttt tggactgtgc
    16681 ctgcaaagga tggagagccg gatctgtggg tgtgcatgtc ctgtttgagc gaggcatttt
    16741 gccgcaaggt acccatgccc gcctgtccca cctgtcacgg ggtctcgacg tatgaagcgt
    16801 ttaccttggc agcggtgcag gattggggga ctgaagagtt gatcgccaaa gccgtagctg
    16861 ctcagcagga agaggcggcc ctcagcgcgg cggcctctgc ggctcctcct gtggagtccg
    16921 tgcagtagcg agtgtcctct ccgggagttg gtccgttgga aaatggacgt ccatttctaa
    16981 tcggcggccg ctggtctcaa tccacgacgg ttgccccagt acgaaatccc tacacaggcg
    17041 agacgattgc ccatgtctgc caggccagtc cggcggatgt ggaatcggcg atacagtctt
    17101 cggtcgatgg tgcggcagcc atgcgtcgct tgtccggcta tgcgcggtcg acgctgctgg
    17161 ccaacgccgc ccaggcactc caggcgcggc aggaagaatt tgcgcgtacg atgacggcgg
    17221 aggccggcaa accgctgacg gatgcccgtc gtgaagtcgg tcgcgccatc cagactctct
    17281 cgattgccgg tgaagaagcc aaacgaatcg gcggcgaggt ggtgccgctg gattggtcgc
    17341 cgggcatgga gtcctattgg ggcgtgacgc gacggtttcc catcggaccg atcctcggga
    17401 ttactccatt caactttccc ctcaatctcg tcgtacacaa agtagcgccg gcgttggcgg
    17461 cgggcaactc gattttgatc aagccggctc cccaaactcc gctgacggcg ctgttgctgg
    17521 gcgatctgct ccttaaggcc ggtgtgccgc cgggggggct caatatcctg ccgtgcgaca
    17581 acacgctcgc ggaacagtta gtcgtcgatc cccgcttcaa gctcctgagt tttacgggga
    17641 gcgcacccgt gggatggatg ctgaaagcca agtgcggcaa gaaaaaggtc gtgctcgaac
    17701 tgggcggaaa tgccgcggtg atcatcgaac cggacgcgga tctggagtat gccgctcagc
    17761 gctgcgtcac cggcggcttt acctatgcgg gccagacctg tatttccgtt cagcgcatct
    17821 ttgtgcatga atccattgcc gagtcgttta ccgaacagct cgtggctcgt gtgcgagcgt
    17881 tggcaaccgg tgatcctggc gacgaacgca ctgtggtggg tccgttgatc gacgccggtg
    17941 ccgctcaacg catcgagggc tggattgacg aagcgattac ccagggcgcg cgtctgctgt
    18001 ccggtgggtc gcgcgaagga tcggtcgtac gccccaccgt cttatcccag gtgactgccg
    18061 ggatgaacgt ctcctgccgc gaagtgttcg gccccctggt gacgattacg ccctatcgtg
    18121 agtttgaggc ggcgttgcgc gccgtcaacg agtcggacta cgggctgcaa gcgggaatct
    18181 tcaccaccaa catcgggcgc atttttcagg ccttcgagca gttggaggtt ggcggagttc
    18241 tggcgaacga gattcccact tttcgtgcgg accacatgcc ctacggcggc atcaaagatt
    18301 ccgggatcgg gcgtgaaggt ctgcgctatg cgatcgagga tatgaccgag ccgaagttgc
    18361 tggtcgtcaa tctccggcgc ccgtaaagga aagcgcaggc agagcaaaaa aacccctccc
    18421 cccgtattgc ggatgcggaa caaacttgat acaactttcg cgcgaatcgg cgcatgatca
    18481 cgcgcgcgac caagggcttg tgacaattca tcgagcactc acctggagtg cgttcgacgc
    18541 gggatgaaga gggagaccga catggtacca acacacatgt ttttgacgag aggcgtcgga
    18601 gtgcacaagg aaaagctggc tgcgttcgag caggccttgc gtagtgccgg cgtggcctat
    18661 tgtaacctcg tcagtgtctc gtccattctt cctcccaact gcaaaatcct tccccgcaaa
    18721 cgcggcgaaa aattgttgaa ccccggcgag atcacctttt gcgtcatggc ccggtcagag
    18781 accaacgaac gtaaccggct catctccgcg tcgattggct tagcgattcc aaccgatcgt
    18841 gatacgtacg gctatctttc cgagcaccat gcctatggtg aaacggacga agagtccggt
    18901 gaatacaccg aagacctggc ggctcagatg ctcgccacca cccagggtat tgagttcgat
    18961 ccggatgtgg cgtggaaaga gcgcgagcag gtcttcaaga tgggcggaaa aatcgtccgg
    19021 acgctcaaca tcacacaatc cgcagtggga cggccaaacc gttggacctg cgtgattgcc
    19081 ttggcggtgt ttatccccac ggaaaacatt cccaagagtc ttcggaacaa ggcctagcgg
    19141 cggcgatggc gcttccgtcc gggtggcttg ggcaagccga taattttctt gggatcgacg
    19201 agccctggtg ccatcccgat caggccggcg tgtatgtcct gccggctccc tacgaacaca
    19261 cgtcgagtta catcctcggg tcagaccggg gtccatctgc catcatcgaa gcttcccaac
    19321 aggttgagtt gtacgatgag acgttgcggt acgagccgta tcgcgagtgg ggtggtgtcg
    19381 ctacggccaa cacgctgaac ctgaacgggc gtgtcgatgg tcaagccgtg caggccattc
    19441 aggattttgt gcaaccgcac gtggggcgcg ggaagttcct ggtgaccctc acgggagagc
    19501 acaccggcgc gttaggtgct atccgtgccc atgctcaaca ctattctggc atgtgcgtcg
    19561 tgcaaatcga tgcccatggc gacctccggc aagcgtacca ggacaatcct tacagtcacg
    19621 ccagtgtcat ggcccgcgtg gtgcaggatg gtttgccgct ggtgcaggtc ggcatccggt
    19681 cgatcagccc cgaagaaatc gagctcatcg ataagacgcc acggatcaag acctttttcg
    19741 ccgcctcgat tttggatccg tccggcccct acgaggggcg ggcggctcgt tggattcctg
    19801 aggtgttggc ttcctgcccc gggcctgtct atctcacctt cgattgtgat gggctggatg
    19861 cgtcgcttgt tcccgccttg ggaacaccgg aaccgggagg gttaggttgg tacgacaccc
    19921 tcgcgctcgt gacggcgctg gccaatgggc cgggcatcgt cgggatggac atcagtgaga
    19981 ttgcacccat tgaaggcttc gtcgcgcctc agttcagcat tgctcgcttg atttatcgta
    20041 tgctggggcg ggttcgggcg ggccgccggg ttcattaggc ggatggtgaa tcgctctccc
    20101 ggctctcatc tcacagtgct gagacgtcag acgtacagtt cgtagcatcc tccctcttca
    20161 ttcgcgcggc cgccatgcgc atcaataaat tctttactga acagggcttg tgttcacggc
    20221 gtgaagcaga ccggctggtg gccgaaggcc gggtcacgat taatggtgtg gtggccaagc
    20281 tcggtgatca ggtttcgtca caggatgttg tcgcgcgaga cggaacgatc ctgcagcggg
    20341 gcaaccgtgc ggtctatctc aaatatcata agccggtcgg ggtgacgacg acgaccgaac
    20401 tgcacgtcac gcggaacatc atttcggaaa tcaatcatcg cgagcggatc tttcccatcg
    20461 ggcggctcga caaggattca tcgggtctca ttctcctgac caatgacggc gatatcgtca
    20521 acgagatctt gcgggttgag cacgggcacg agcgggagta ccgcgtagaa gtggatcgcg
    20581 agttcgacga cgccttcctc tcgcgcatgg cagagggtgt cgtcatatta ggagagcgaa
    20641 cgcgtccatg cctgatggcg cgagtagggc cgcgtcgatt caggatcatt ctaaccgagg
    20701 ggcgcaatcg ccagatccgc cgcatgtgcc aggcactcgg ctatcgggtg gtttcattac
    20761 atcgcgtgcg gattatgcac atcaccgtcg aagggcttca tgcgggacag tggatggact
    20821 tgagcgagga ggaacgtcgc cggctgttcc aggctctagg ccggccgccg gaatcgtcgt
    20881 tgtgagaggc aagcggctct actcggcgcg gagtgccgta ttggatccgc ctccaggcag
    20941 gtccaaggag tcgggacgga cggattcttc aggtctggat gcggagagtg gcgctctggt
    21001 cgttcgactg aggaacagat tcagcaacgc gatgaaaaaa ctccctccca caatccaata
    21061 actggtggag cggatggttt tcgtgccttc gtcgcgcatg tctttggcga cttccgcgac
    21121 ggtttccagc aggcggtcca aggccagact tacttgcatg actttcggtc cgccattgtc
    21181 tgcggcatgg atttccgctt tgcgcctgag ctctgtcgcc tcgccgggtg ttcccgcctt
    21241 ccattcctgc gacaagagat cgaccgtttt actggcgaca tggaagtatt ggtccaggct
    21301 ttcacgtacg gcctgaatgt ctttttcttc gctgcgtcca ctacgggaga cgcgcaagcc
    21361 tgctgccgca taccgatcca ccgcatgttg aatgcgagcg cgttgcgccg gaagtgattc
    21421 ggtaatgcgc tcgaagaccg cctgattttc cgcttccagt gagcggatga tggtgttgcg
    21481 atagcggatg acgtcggcgg agatgtgggc gaggtctgcg gcccccagcg tgtattccgt
    21541 atacatgatg cgcaagtctt gatcgatgcg gctcagcgcc tggccgctga tccatcccag
    21601 tcccgcgatc aggaggctga tgatcaattg attcgtgctg ggaaaggaca atttgaaacg
    21661 cgaggcaaac gacacggacg tccctttcgt cgggccggaa acgatgtgca tggcccggga
    21721 ggttgaagga ttcagtctag cagatcgtgc ctcaagaaag aaatcgacgc cggttttgcg
    21781 acaaggccca tcaatcgagg atgacgcggc gctcgaccag ttgcccgact tcggggtcat
    21841 tggacacgaa cacaatggac cagggctcct ctttagcgca gagccttcgc aggagagtca
    21901 gtcgcacggt cgggtcgata ttgtggaggc tgccgttgaa aatcagcaat tccggacggg
    21961 tcacgatcat cctggcgaga aggatgcgga gcacctggct gcggctcaag ttcgccccgt
    22021 tcccatgcac gaccgtttcc agccccaggg gcagcgcgtc gatctcctcc tccagttcca
    22081 cgaatcgcag cgcccagctc agatcggcaa attgaatcga tggacgctgg agggtcacat
    22141 tttcctccag ggttccatcg aagagggtgg gatgggaatc gagcatcagg cctctgcagc
    22201 gattcacata attgagtgac acgtcgcgca ggtcgatgtc gttataacgg atgacgccgc
    22261 tggtgggatg atagagcccg gccaggagaa gcgcgaggga cgttttgctt ttgctcgtgc
    22321 ccgtgagcac actgatcttt tcgcccggca gcacttccag attgaaatga tcgaacagca
    22381 tcggtccctc agggctggca aaggacacgt ctttgcacgt cagtcgtact ccctgcagag
    22441 tgggattcgg cagccaggcg gcgatgggac cggagacctc ctctttcggc atttcgaaca
    22501 gcctggagag ctcctgcatg gatgtggcga cgtagatagc ggcgtacatg cgacgggcga
    22561 cgatgtcgag attgagaagc agggtgccga caatgacctc cgccgcaacg aattggccga
    22621 gggtaatgtc cgcgagggag agtaaccatc cgccgaggcc gatcatgctg ctatgggcga
    22681 tggcctgaaa gatgaccgta ctcttgtatt gcgccccggt cagaatatcg gatcgggtct
    22741 tcctcgccat gacataggcc tgcacgagcg catcggtctt cttgagcaga agcggcaggc
    22801 tgtcggtaga tttgaaatgg agccggttga tgccgatgtc ctgcaaccag tgaaacgtct
    22861 ggtagtgaag gttggagact cgttgagtaa tccggagtcc tccgcggcca aaaaaggtca
    22921 gaagaaaagc aaatccggtg atcaggaaga tgttatagcc gaggaagtag ggatggtaca
    22981 tgatcaggat cgtcatgccg atcatgcccg acacgaacac gttgatgatg tcgaccagca
    23041 tcgcgaccag cgcacggggc aacagctctg cttcgatgaa ggtgttggtg tgttgcggaa
    23101 ggaagacgtt ctcccggaac cggggcaggg cttcggtaaa ggcgacagcc agcctagtat
    23161 agatccgctg caccaggatt tccaccgcgc gtccctgcaa gacccggaat gcccccatca
    23221 acagcaacgc gcctaacatg atggcgacca gcgtcacgat catgatcggt tgaatggcga
    23281 aggcgaaggt gttcacgagc tcttgcacgg tgagcggcac gatcagcgca aacagaccga
    23341 tcacgactgc ataagaggca atgatggcca gaatgcgccg ctcgagtccg accaggaggt
    23401 tgagacgcga aagaatcgtc caggctgaag gtgtggtgat cgttgattcg gtagcgatgg
    23461 ggttcacgcg atagctcgtg tcgaccgatg gtggggcgca gtgtgggcga atgccgcatc
    23521 tctgtcaaga acgggaaaca cggtggtcgg cgggtgggaa agaagcgaga gcccgaggcc
    23581 agcagcctag gtgacggtgt ggcaggccgt tagttcagca tgatccggcg gtcgacatgc
    23641 ggggtgagat tcggatcgtt cgagacgaaa atcaccgacc agggttcttc cttgctgcat
    23701 aagcggcgga gaatcgtctc gcgcatcgcg ggatgcatgt tgtgaagaat gccttcgaaa
    23761 atgaggagct gcgggcgtcc tagaatcgct cgcgccacca ggatgcgcac gatgtgcgta
    23821 ggggcgaaga ttttacccgc agaccgcaca tgtgttttca agccatgggg gagggcgtcg
    23881 acttcttctt ccagttcggt gaagcggagg gcccaccgca catcgctata gggaatgtag
    23941 tcccgcccga gcagaatgtt ttcttccagt gtcccctcaa acaacgagag ttgtgaatcc
    24001 aggatgaatc ccctgaagcg attgacggaa tccaggttga gatggcgcag atcgacgccg
    24061 ttgtagcgga tgacgccggc tgtcggtgat tcgagtccgc cgagcacccg ggcgagcgcg
    24121 gttttggcca tcgtcgtatc ggcatagatc ccgatttttt caccgggcgt cacttccagg
    24181 ttgaagtgct caaatacggg cgggccgccg gctggcgcaa acgtgaggtc tttgcacgtg
    24241 actcgaatgc cgtgcaccgt cgggtcgggc aagggaatgg acagcgtggc ggcgtcctga
    24301 tctttgggca acgagaagag gaagctcaat tcggtcaagg cggtgagaaa ataatagata
    24361 tgccccatcc gcttcacgac gccgtcgaag cttgagagga tgccgctgac cacgacttcc
    24421 gcggcgacga attgtccgag cgtcagctgg ccgattccca ggagccaacc agccgttgcg
    24481 atgaggccgc tatggacgat cgcctgccat ccgagtgatc ccagatattg ccggatgaga
    24541 atgccgaagc gggttctgcg caccccgaca tagttgtcca ggagttgatc cgtcttcttg
    24601 atcagcagcg cctggctatc ggtggacttg aaatggagca ggttgtacgc aatttcctga
    24661 aaccagtgca gggtgtcgta tttggcgtgg gacatgtcca tgtcggcctt cagcccgcca
    24721 tggctcatga gaaagaacac cacgttgaac ccgaccagca agatggcgtt aaacaacagg
    24781 aagtacgggt ggtagaacac gaggatggtc ataccgaccg ccccgccgac gatgacgttg
    24841 atcaagtcga ccagcagcac cgatacggcc cgctgcatga gaatggtttc gacgaagtaa
    24901 ttggcgaatc gaggcttgaa gccctgatac cgcaaatgcg gcaattgttg ggccatggca
    24961 atggccaccc gggcgaagat gcgccgctcc aggacttcca ccgcataata ttggagggcc
    25021 cggaaggcgc cgacgaagag caggcccgcc accatgaccg cggccagtgt gacaatggtg
    25081 atgggctgga gcgcgaaggc gaacgtattg acgagttcct gaactgtgag aggaacgatc
    25141 agcgagaaaa gcccgatggc gatggaatag gaaacgatga gaccgaggat gcgccgttcc
    25201 agacgaaaca ggaggcccag atggccgacg accgcttgaa acaaattgct ctggttgtcg
    25261 gaatgatcct gggacatcct gctacccctg ccgcgcctca tcaacccatc ggccgtgttg
    25321 cggcgatggc cggatgatga ttgttgcggc ggcctgtccg cgcaggccta caccagctaa
    25381 aacctatcag agcgcaccgt aaaatactac tatttggaca agctcgcacc gacccggtag
    25441 ttgaccggaa cggctgaggg ctggctcctg gcccaagatc ccgtcgccca ttgatagagg
    25501 gcgagagcct tctggtaatc cgccttggcc cggacgagtt gcatctccga atcgacggaa
    25561 ttccgctccc gcaggttcac aaacagcacg ctcgttgcgc ccaggctgaa ccggaaacgt
    25621 tcaccttctt ccaaggtctt ggccatgcga agtgattcgg ccgccgccgc gacccgttcc
    25681 ttcgcccgtt cgatggccga cacggcattg tcgacatcca cgaccacttg ctgttcccga
    25741 tatttctgca ccagcaccag gcgatcggct tgcgcctggg cctccatcac ttcaccccgg
    25801 cttctccgtt gaaggatcgg gatacgcagc tcggccccga accgatagcc caatcccagc
    25861 acaaatttct ccggagccct ggcgggcgcc gcttccagat caagactcgg caggagattg
    25921 ttcttggcga gttccaaatc gatattatta accttggctt caatatcgac ttcgcgaatt
    25981 tccgggcgat cgcttttcgc ctggagttta tccgctttga cgacgtctgc cgtcggggcc
    26041 gctgcgggag ggggaaaatc cggaacgcgc tcgagctgcg gggtcgtcgg cgtctggttt
    26101 tcccagagga acatcgacat tttatattgt tcctgctcca cggctcgctg cgcggcgatg
    26161 gagatttcac ggcgacgctg gacttcctgg cccgcctcga cgacgtcgag gggggccacg
    26221 gctcccgcct tggctctgcc ttccacctgt ctcaaccgtt cctccgcgac gccgagggcc
    26281 cggcgttgca cgtcgacaaa cttggcggcg gagacccagt cccagaattg gctcgcggcg
    26341 gccagaaaga gatcctgtct ggtttgcgat attttgacct cggcgcgagg atcagccagc
    26401 tccgaacgct gcaactccgc gttttcgggg ttgatcatga gccctttcaa cagaggcaga
    26461 aagccgccga gaatgacctg ctggttaccg tttccgaaag aaaggtcagg aatcttcgca
    26521 tcgccgatgg cctgtcgaac gcccgcgctc ccgcggaatc cccatggggt ccgcgcttcg
    26581 accaaggtat cgttgaatcc gacgctctgg gttttggagg tgctcttgtc gatgaaccgc
    26641 tcgatctcgg tatcgttcac caggaccggt tcgaatgcgc cgagggcctt gagcattttc
    26701 ccgcgtgccg acgttttttc cgtaccggct cccttcagaa ggggatgcga acgatcgatc
    26761 caggcgtgaa cctcgttcaa cgacagagga ataggcggaa gggtttttgc tttacccgta
    26821 tcctccgcct gggcgctgct ctctggaagt ccccagagtg cacaagcggc aatgaccagg
    26881 atccatgcat agttcttcat gaaacgcact cctttctgag gtcacaagac agagaatacc
    26941 cgtcgaaggg tgtccgtgct ccggcatgcg ccggggagac gggaccggtt acttggcccc
    27001 gcgaccggct ttcggcagga gggtatcgat caggcttgga ggccgttcct ggtagtcggg
    27061 cggaaagaga ttgaaccggc gccagagctc gtaccagagc gggacgcgat tgagaatgac
    27121 ccatcccatg gccttcgtgc cctggcggac gtgttcttgc ggaggccagg gacgttcttc
    27181 gagatcggga acgacccaga agcggaaatt acccttgccg tcgtcgacct ggtcgatgac
    27241 cttgatgacc ccgttgtacg taccagccat catttccggc caggcgggca gggggatggc
    27301 gggaatgccg tagaagagaa ttttgacctt tcggcccaca ttgagtaacg gggcatcgat
    27361 gccgtcggcc accatttcaa tggccctgtc cacactgttc ggagaaatcc tgaccagttt
    27421 ttcaccgggc cgcacggttt cgccggcgcc ggcctgcgcc atttttacga cggtaccgtc
    27481 gatgggcgag agaatccggc tggcaacccg tcgttgattc gcgttggaca gacgcagaga
    27541 aacatccgcc acctgatcgg cggcccgcgc cgcttctccg atcgaggcat ctcgcgcggc
    27601 ttctgcgtca agcagccgtt gaaggacttc cgcgctgacc tgttcccgtc cgaaactcaa
    27661 agccttcatg ccctgttcgg cggcagccag gttggcttgt gcaccctgca aatccgcctt
    27721 gctggcgatg gcggcttgaa tcgtgagttc taattcgcgc tgggagacca ggccctgttg
    27781 cgccagctgt tgatgccgtt ccacattgag ttcggctgtg gccacggcga tcttggacgc
    27841 ttcgattttt tgccgcgcct cacgcacttt gctttccgct tcgagcaccc gcgcgcccgc
    27901 ggacggaacc gcggccttca cgaggttttg catttccttg atacgtttat cgagttgatc
    27961 ggcgcgactg agggcggctt tccgcgtatc ttccagcgcc tgcttgcgct gttcgaggag
    28021 gttcaagaga tccggagcca tgaagttcgg gtcgtagtcg tccagttcga gaatcacgtc
    28081 gcccgctttg acccgcacac cttcgaagat gtgccacttc ctgatgcgtc cggtaatctg
    28141 tgcttcgatg tcttgcggac gttcaaaagg cgtgtaggcg gagagctggc cggttaccgt
    28201 aatcgtttgc gtccacggga cgaaagccac gatcaacatg aagaagccga ccaggagaat
    28261 ggccagccgt gaagagaacc gcaatcgttc gggaatttgg acggcttccc aggactggag
    28321 cttggtggac gtggccagat catcgacggc catttcatcc aagcctgtac gctgggtgac
    28381 gagggagcga aatcgggttt tgagtccctc acggatacgc tgcacgtaac ccctccacgc
    28441 accgtccact gtagagatgc gcacccgtat catagtggaa gacaaaggag ttggtcaatt
    28501 caatgaggcg ttccgtcgtg cttcggcttg acccttcgac cggagaaggg ctatggttcg
    28561 ccgcatcatt ctttccttca gccagaggtg tcgtgtccat gccgaagaag aagcgtgcgg
    28621 atcgggcgtt ggtcggggtc ttgggcggca gcacatcgga ctttcccatc cttgaaaagg
    28681 ccgtggccat gctgaatgag ctcgggattc ccaacgagct gctcgtcgtg tcggcccatc
    28741 gtaccccgga tcgcctcttc gcctatgcgg aacaggctgt ggcgcggggg attcaggtca
    28801 tcattgccgg agccggggga gccgcgcatc ttcccggaat ggtggcggcg aagactctgt
    28861 taccggttat cggcgtgcct attcccactg aacatctccg gggcttcgat tctctactgt
    28921 cgattgtcca gatgccccgg ggaattccgg tcgcaacagt ggcgatcggc ggcgcggaaa
    28981 atgcggccat cctggcggcg caaattctgg gagtacagtc cgcttctgtc aggcaacgcc
    29041 tcgaacgatt ccgtgccgcg caaacacaaa aggttctcga ttcccaggat gacgcccggt
    29101 tgtcccctcc cttcccgatg tcacgcggga ccaggacgag ccgcaggccg tgaatgtcac
    29161 ggtgattgat cccggcgcga ccctgggtgt gcttggtgga ggccagctcg gcgccatgtt
    29221 cgccaccgct gcccgccgca tggggtacgc ggttaatgtc tgggatcccg atcctgaagc
    29281 gcctgctcac cggatagccg accattcact cattcgcccg ttcaccgacg ttgacgcgcg
    29341 taaagagttc actcaacggg ttcgtgccgt gacctatgaa tgggaaaacg tccctgccga
    29401 cctctgtgag caattggagt gcgagctgcc ggtacgtcca tcaagccggg tgctccgggt
    29461 gattcaggat cgcattgaac agaagacgtt tttgcggagt cgcggattgc ccgtgccggt
    29521 cttcgcgaca ctagcgtcgc cggaggaact cggccggcag accatgcctg cttatccact
    29581 ggtgtgtaag acggctacgg ccgggtatga cggcaaaggg cagtggaaaa tccttcgagc
    29641 cgaagattgt ccggcggtgc aacaggagtt ggcccgtact cggaggcctg gttctcgatg
    29701 gattttggaa gaatggcttc cgtttcagcg tgagctatcg attctggttg tgcggggtgc
    29761 cgacggcgcg tcgcaaacct accccttggt ggacaatgtt catgaagagg gcattctccg
    29821 tcagacgatc gtgccggccg atgtgccgca gccggttgcc gacctggccg gcaccatcgc
    29881 gtgcgacgcc gtgcaggcgc ttgacggggt gggggtattt tgcgtggaat tgttcctcat
    29941 ggccgatggc cggttggtga tcaacgaaat cgcccccagg ccgcataact ccggtcacta
    30001 caccttggat gcctgtacgg tctcgcaatt tgagcagcag gtgcggacct tgtgtggcct
    30061 gccgctcgga gagatccggt tgctcagtcc cgccgtgatg ctcaacctca tcggtgagga
    30121 tgttcgactc gtgacgcact cccctgcagc gcaggatctt ctccgcgtgc ccggtgcgat
    30181 cgtccacctg tatggaaaac gcacaattcg accaaagcgg aaaatgggcc acatttcatt
    30241 tctcgctcct acgcgcgcgg aggccctcac cgccgcgtcg accttccgct cgcggtttgc
    30301 tccgattcac tcgtaaaaaa acgacagcag gcgcgatcag gtttaggctc ggtgtgcgtc
    30361 tggatggtat ccgtttctgt cggttgagat cgttcggccg ctctcagact gtccgttttt
    30421 gtgagacggg gagaggtcac cggccgttca tggccaccct tcattctcct cctcctgaaa
    30481 ttgcgaagac ttgccaggat ggcaagacgg tatgggactt gctgtcatcc ccgctggttt
    30541 cacacttctc tccgaagggc aggcacagcg gtgagcgttc cactttcctc cgatctcgat
    30601 caaaccgggc ggcatcccac catgcgagga ctgggccgat tgttctctca ttcgcgagtc
    30661 cgcgtgatgt ttctgcaaag cctcgtggcc ggcattctgt cctatgaatt gcttgtgagc
    30721 aacgagacga tctatgggca gaacgtcagc cggatggtgg cgctcggatt gatgctgatc
    30781 agtatgggaa tcatcttgtt gccgaaagcg gcattagaga gcacctggtt tcccggcgca
    30841 ttgatcagca tcaataccct gctcgtgaca gggaccattt acctctccgg aaatgccagc
    30901 tcggaattgt atctcacgta ttttctactg ctcctcattg cctcctctgc gccttcactg
    30961 aaacaattac tcggcctctc cgtcatcatc tgtgccggat acggcatctt ggtctatgag
    31021 catgcgatgc agtccggcac cctggcggtg gggcacctgc tcggtattcc tgttttgctc
    31081 atcatgtcgg tgttttacgg cctcacgctg gaaacagtgg cggtcgagcg ccggcggaac
    31141 cgtctgttgc gggaagatgt tcaggggttg aaacagtcgg agcaggttct ggaggaacga
    31201 cgaacgcaac tggaaacccg cgtgcaaggg ttgaagcaag ggctgtcgcg cgccaaccag
    31261 gaaattcgcc aaggcatggt cgaacgcacc gggctggagc ggcagttgcg cgaagcgcag
    31321 aaattcgaag cgatgggacg actggcctcc aggttggcgc atgaattcaa tcaggtcttg
    31381 gcggtgatcg gggctcagac cggggccatc gtttcaaagt tgaaacccga tgaccccctg
    31441 catgggccgg tggaagacat cttccggagc ggagagcgtg cggcaaccct caccgcgcaa
    31501 ctgctcgcac tgggcgtgca cgaaacgagc attcgcgaca ccttgtcgct cggcgaggcg
    31561 attgtgtcga tgcgcgaggc gctccaggca ctgcttcccg ggcggattga tgtgagggta
    31621 cctgacgagt ccaagccggt gctggtggaa attggtcatg accggttgga gtgcctgttg
    31681 ttgcacctgg tggcgaatgc gcgagatgcc atgccgagca gcgggcgggt ggatatttcg
    31741 ctggaagtgg tcaccggaga gttgttaccg gcagagcaac tcgaaaaaaa tccccggaag
    31801 cggatggccc gcattgccgt cagtgatagc ggcggcggga tgaatctgga cgttcaagcc
    31861 cagatgttcg agccgttctt ctcgaccaaa gagactcatg ccggtctggg gctcacgttg
    31921 gtctatggaa tcgttcgtca atgcggtgga gcagtggagg tgcaaagcca gcccggacaa
    31981 ggcacgacgg tgcgtgtcta tctgcccctg gtcgagcgga acgccgtgct gagggacagc
    32041 agggtcctcc ctgaggcgct gtcgaagggc gctgaaacgg tgctcctcgt tgaggaagat
    32101 gaaattgcca ggaagctggc cctgtcgacc ttgcacggcc atcgctacca tgtactcgag
    32161 gcggggtcgg cagtggaggc actgcttgtc gcgcaacaat accaaggacc gattcacctc
    32221 accgtcagcc acctttcgat gcctgaaatc agtggccggg agttggccaa acggttggtc
    32281 ctgcaacatc caaaaatgaa ggcgttgttc gtgtccggat tttccgacga gacgattgcc
    32341 agtcatcgag tgaatagaaa atacttcctg cagcagccct accggcagca cgatctggcg
    32401 gggaaagttc gtgaattgct ggatgtctaa gcgaggaatc gcgacgctag tatcgaaatg
    32461 actcggtgcg gccgggtttg aaaaatcgta cctccgggcg cttaaagaag ccgatcagca
    32521 ccatccctgc gataaatccg ccgatgtgcg caaagaaggc cacgcctcct ccctgcgagc
    32581 ccaggctcgc cccaccgttc agcaactgca tgacgaacca caagcccagc acgataccag
    32641 ccggcacgta ggtggcgccc accaccgggg cgatcaacaa gacacgggca tggggaaaga
    32701 gcagcagata ggcccccagc acgcctgaaa tcgctccgct cgcaccgacc atgggaaccg
    32761 tcgatgtcgg gtcggtcaag gcatggctca acgccgccag gattccacat cccacataga
    32821 agacgataaa tctgacatgt cccatgatgt cttcgatgtt gttcccgaag acccagaggt
    32881 acagcatgtt gccgatcaag tgcatccagc tgccatgcag aaacatgctc gtcagaacgg
    32941 tcgcatacgc gggtatgcgt gtcagttccg gtgggagcac ggcctggccg aacacgagtg
    33001 aggggatggc cccgtattgg tagacgaagg cttcaccgga ggttgacccg agggagcttt
    33061 ggtacaggaa gacgagtgag caggcgacga tgaatgcgac ggtgacgaca ggcgtgcgtt
    33121 cggtcgggtt atcgtcactc aggggcagca tcgcaacacc tgaaacacaa gggctatgag
    33181 gcggccggtc gcggcagccg aggatttgcc tgggggaggc cgtcggcctg cagcggcacc
    33241 gaaaatccag ccgcatgggt atggccaccg ccgccgaagg atgcggcgat cgcgccgacg
    33301 tcggtgccct cttcgcggga acgcatgctg aagtatcggc gaccgttacg atcgtgccag
    33361 atcacgcaaa acggatgctc ggcggacaac cgttcgccga tttgactggt gaggacggag
    33421 ctctggaccg acggcacggt ggcgccttca aactccacca gcgtcgcgtg ggaggccagt
    33481 ttcgtcacga gttcgttttc gtagcggaga atggcccggc cttcccgctc caactcgcgc
    33541 tgttcgaaac gcgtccagag ctcgaagtcg aacgggtgtg acgccagtgc tgcgctgatt
    33601 tccctgctgt tcggaagcgc ccagtgccat aaatccttgt cttggatgta gcggagcaac
    33661 cagggggccg gttcatcgtg ggcccattcc cagcccagta ctgcgcccga cttgttgaga
    33721 tcgaaatacg cgtaggggag gtcggccagt gcctgttccg cagtgatgtg atggtccaga
    33781 acgaccaggc tggccgcatc cttggccatg gcttccaagg ttgagcggtt gtagctgaaa
    33841 tccaccatga caatatgatg accggcgagg ttcgtcggcg gcgcttcgcc gtgtttcacg
    33901 gggcgatact ccgcgtcggg ataccggcgc cagatggccc aggcggcgcc gaacccgtcg
    33961 gcacattccg cgtgatacag gatcagtgtg ggaggagagg aagacagggt tcgcatggtg
    34021 gctgcgctca taggccaacc accttagcat gtccgttcat ggagactaaa gagctatctc
    34081 aggattaggc gagccggcgt ggctgccggc ttcgcctgca ttcgctcagg tggtcgccgg
    34141 cagatgattg aggtgattga tcaattgcct gaggcggcct gcattgcggc gattgaagga
    34201 aaatgtcagt cgtgcaagat ctttcaatcc cggctcgtca atttcttcgg ggagtgaggg
    34261 ccgttgctcg aacgtgcctt ccgtcagcat gtctttgaat tggtgctgta cttcttggac
    34321 ttgctccgcg gtcaaggggg tcttcagtct gatggcgagc tggcgtccca cgaatcggag
    34381 ggagtgatag cgccgataaa acgtgcggat ttccgtgacc gcatcttcca cgttgtcgac
    34441 gatggtgaag agggaaaggt cttcggcgtt gatcagacgg cgcttgagca gctgatcggt
    34501 aataaaagag ttccagtggt tccaataatc gcagcccggc gcctggaggc acacgatcgg
    34561 cttcggatcg ctcttgccgg tttgcacgag ggtcatgatc tcaaacccct cgtcatgggt
    34621 gccgaacccg cctggaaaga gcgcaatcgc gtgtgactct ttttgaaaca tgagcttgcg
    34681 cgtgaaaaaa tacttaaacg tgacgagttt gggatcatcg gcgatcacgg cattggcgcc
    34741 ctgttcgaag ggcagcatga tgttcacccc gaagctgtgt tcgcgaccgg ccccttcctg
    34801 cgaggcccgc ataatcccat cggcgcctcc ggtgatcgtc atgtagccct cttccaccat
    34861 gcgtcgggcg aactgaaagg ccagttgata gttcggatcg tcggcgggcg tgcgggctga
    34921 gccgaagacg ctgattttca gtcgatcgcg atatccttga aacacgcgga aggcatgccg
    34981 gagttctttg agcgcccggt tgacgatctt gaggtccagg acatccagtt gcgattcaga
    35041 gagccggatg acgccggcca ggatctcttt catgagcgcg gcctggacat cgtcgtctgg
    35101 ccgatcgagc agggtggtga tttgtgtaag aatttcgtct ttggtcggaa cgggtttgga
    35161 acgagcggcg ggagttttca tcaatagtca cctaacgcac ggtaaactcc ggacgggcat
    35221 cgccggagaa tgaaatcata acaagccggg gagctcgaag caaaagattc atcgggagga
    35281 acttaggaga gatccgaggc acgcggcagg ttttgcaaca cccaggcccg aattttcgat
    35341 tcgtcggacg acgagaggga caagaattgg atatcgatgc cggaataggt tcccagcacg
    35401 ttccggtctt cgtggaattc cggctcatag ttgctgttga gaatggtgcc ttgcagcgag
    35461 aagctcaagt ccgttccggg aagaatgaac gacagcacga tactcgttcc gggaagcagt
    35521 aatgtgcgac tttcgatcga cgcgccgaca tggctgagct gccggattgt ggcggtatgt
    35581 tttgcccctt cgccttcgcc gttggtcacg cgaatggtgg cgggaaggca ggtctcgcag
    35641 cgtttcgggg ccagggccag gtcgcgtgca gtcaggatgc cgaccggttg tccttgtttg
    35701 gtgacgatca ggagggaggt gccggtcgag gccatgacgc tggtggcatc gtcaagaatc
    35761 tggtcacatt cgacggaatg gaccggcttc gacatgatgg accgcacctc cacatcgtgc
    35821 ggttccagac cctgggcgac gacttttttg acgatgtcgc cggaggtcat gatgccgaat
    35881 gcggcctcac cgtctttgac cagcaggcag ggcatctgtt cccgatcgag gagcgaggcg
    35941 gcctcgctga cagagacgtc cccgggaatc tgtaccacgc cgggtgtcat catatgggcc
    36001 accatagggg ttgtcgtatg agtcggtttg tcgcggagta tggagccggt tcttgtcata
    36061 gtgtaagcct atttctcgcc gtgagagggg ctaacggtgc ggcccggcga ccaccacgtc
    36121 gaaagaaagt atatcagatg gatgtgccgg acccgtcttc ccatcttccc ttgtcaacca
    36181 agaggttagg cgcttagact gcacagaaac tatagtcact atggttgttt caaaatatgc
    36241 taaatgcgat ccgatgaaaa gcgaattcac agaacgtgcg catcgccttc ctgcacatgg
    36301 tctcgggctg tccgtggacg tgtattcccc ggacctgctg gagctggtcc gtgcacttcg
    36361 tgatgcagcg ctggagccgg actatctgga gattttcaaa gccacgacgt ctgcccaaca
    36421 atggatgcgg caacaattgc cggatctcaa gctgacctat catggtgagg ggttgtggag
    36481 cacgcaaccg gaattctgcc gcagcggttc cggccgacag ggagtggcgg aagcctgtgc
    36541 gcagctcgcg gcgttgcgca gcgcctggct caatcacgaa tgcgccacga agcagatggc
    36601 gggttatgcg ttcgggacct atctgccgcc actctatacg gagttgtcgg cgaggatgac
    36661 tgcggagaat gtcgcctatc tccaaagcca agtggatcag gatgcgcata ggcgtgaaat
    36721 cgatcctgcg ctggtactct tagagatgcc gccgctcacg tacttcggct gcggcgcgct
    36781 tccaattccg gattttttcc gtgcggtcac cgatcaggca tcctgcggct tggtgttgga
    36841 tatcggacat ctatggaccg tgtatcgcta tacaggaagc tggcaacgac agcggttgga
    36901 agagttcgcc gcccagttcc ttgatgcgtt tcccatggag cgggtcatcg agatccatgt
    36961 cgcaggactg gccgaactga cggttcctca cccttctgga attgacgcga gcgggaatgc
    37021 attaccctat tggattgatg cgcacggggc tccgatccca ggcgtcttgt ttgatctgtt
    37081 ggcgcaggtc ctgtctcatc ccgggctgac atccttgaaa ggcgtcgcac tcgaggtcga
    37141 tacgaagccg attgcgatga tcgtcgagga gttcaagcaa tttcgagaac gatttggaac
    37201 gacggttcaa gcaatgctcc atcgtgacag ccctgcgcca gtgcaaccgc agcgatcgcc
    37261 gaatgctcgt tcggctgagc gacggcaact gacttcggaa gaagaagcca cattgcagca
    37321 acagtacctg cactatgtgc aactcgtcac gacacaagat gatccctcct ctataaggaa
    37381 cctctcgttg ctaggcgggt cgctggacga tctcaaccgg tatcgcgaga cctacctgcc
    37441 gcatgaattg ctccattggg gcggcgaatt gcaggatatg tttccggaca cctgtcgtgc
    37501 cctgacggaa agtgatgtgc cgttggatcg gtttgttgcg gtctggttca gcaaacccag
    37561 acccgagcag gacgactatg attttttcct gctgaagatc gaccgattcg tggaattcgc
    37621 gacacacgcg tgtccgatgg ctgccatgat cgtgatgcgt gaagccgaag agctacgggc
    37681 agcctatcgc caggcgaacg aaccgatcgg ctcatcccag gtgcgcgcat gaatttttgg
    37741 acgacgctcc cgcagcccat tctcggtttg gctcccatgg acggggtgac ggatgccgcg
    37801 ttccgtcgcg tggtggcctc tcaagggcgg ccagacatca cgttcaccga gttcaccaat
    37861 gtgaatgaaa tctgccgcgg gccggaccat ctgctgtcct cgctaattta cagcgaagcc
    37921 gagcggccca ttgtggcgca gctctacggt aaagatccgg agcagttcta tcgcgcggcc
    37981 caggtggtct gtgagctggg gtttgacggg ctcgatatca atatgggctg cccttcgaag
    38041 agtgtggcgg cgtccggttc aggggccgcg ctgatcaaga cgccggatct ggctcatgcc
    38101 attctgcgtg ccgcgaggca gggattggag gattgggccg gtgggcagtc gatccaccag
    38161 cagggattta aaccctctcg cattgaattc attcacgagc tgaatcaccg gcgttccggc
    38221 tctcccgtgg tgccccgccg gctgcttccg ctttccatca agacgcggct ggggttcgat
    38281 tgtgtggtgg tcgagcgatg ggtggaacac ttgttggaag ttcgcccggc cgccattacg
    38341 gtgcacggtc gaacgttgca gcagatgtac cgcggtgccg ccgactggac ggctattgcc
    38401 gaggcggcca gggtggcccg tgatacggaa accttgattc tcgggaacgg cgatctcaat
    38461 acattggttg atgctgttcg acgtgccgct gacagtaggg tgcagggcgt gctggtgggg
    38521 cgtggaaccc tcggcgcgcc gtggttcttt cgtgaaaagg agcaggcacg tcgcgcgttc
    38581 gtcgaacagg tcgatctgtc aacgcttccc gccacgatgt gggaaccgcc ggtgtctctg
    38641 cgtcaccgca tgcaggtcat gctggaccac gcgcggcagt atgaggcgat tgccgggttg
    38701 gagtgcttcc gctccatccg gaaacatctc gggtggtact gcaaagcatt tcctcacgcc
    38761 gcggccatgc gggggaaaat gttcggagtc tcgaacgtgc aggatgtcga acgcatcgtc
    38821 gcagaatttt gccaggacct catactggaa gaagctgccg gatctgatgc cgctcccata
    38881 gctcctcaat tcccataccc ctgcgcatcc tgactccatg cgattgattc tggcttccac
    38941 ctcgccgcgt cggcacgaac tattgactct gttaggggtg ccgtttgatg tcgtcgctcc
    39001 gcccttcatc gaacaggtcc agccccactt gtcggcagac tcgcaggcgg tagaatttgc
    39061 cgccggtaaa gccaggtctt gtgtgtcttc ctgtcccgat gccctcattc tcggaagcga
    39121 cacgctcatc aatctgggct ctgaggtgtt gggcaagccg ctcgatttag ccggcgcgga
    39181 ggtgatgctc cgccgtctgg ccgggcagac gcaccgcatt gtgacagccg tggccctggc
    39241 cgggcctgag cctgagcgat gcgacgtccg gctggcagaa gtgctcgtga cgatgaaacc
    39301 tttgaatgaa gccacattgg ccaactatct gcggactggc gatagcctgg gtaaggccgg
    39361 ggcctattcc attcagggag gcggggctgc atttatcgag cgcatcgagg gcgatttcac
    39421 cgcggccgtg ggattgccgc tgcagcttgt cgctgagatg ttacggcagc gtggactgtc
    39481 ctgtccggtc gacatcgatc aactgtacgc cagcagaccc tatcccaact gggaccgctt
    39541 tcgtccttag ccaggccacg tcggtcacac cccttctcac gtcccgccag attgaaatgg
    39601 tcttcccaat ccttttacaa tgcgcccttg ttcagtgacg tgtcagggag gtactcatgc
    39661 gcgtgaatga tggcagggta ggtcggcgtg ggcaaaccca tgcggtggtc gggttggtga
    39721 tcggagcgat ggcattggca atcagcgggc cggcgtgggc gatcgacgtc aagttgtccc
    39781 ccgaagacgc caagaaggcc ctggaagccg ggcgagcgcc gatggaaaaa gcggcttcgc
    39841 cggaagatgt gaagaaaatc ttgcagcagg cttcgctggt cacgcgggtt ggtgcggatc
    39901 ccgaaaaaga cccatgtggg gccagcgcca ttctgcgcac caagcgctac cgcttagagg
    39961 cgttcggcag gcaggaagca gcggaatcga aaaaacagaa aaaagatgtg cgcatgcccg
    40021 acgagtttat ccagaaagtc gtggacatgc cgaacatgga agtggaagtg cagttgtgcg
    40081 gcgatgatga atactttgcc gagaaggctg acatcgtctt tcagcaaaag ggtaaaaata
    40141 ttcgcccggt ggatctcagc ccagctgatc gaggcagaaa aaacgatggc acgggcccgg
    40201 cctaccggtc gcgtttcacc gcccggttcg ggtacgagtc gtttgaccct catgctaaaa
    40261 ccaccatcat tgtgaccttg caggatggtc agaccatgca gttcgatgcc gacttttcgc
    40321 aggtgaaata atcctcagct tccggtgcgg gtctgcagac ccgtaccggt cacttgcgcg
    40381 ccccctccct tctcagtgtt gttctttcat tcgtgcgatg gtgctgacaa ccgaacatca
    40441 cggttgagtc gtcttgtcgc cggcatgcat aatgacggac atggacaggg ccgatctgtt
    40501 ttccctctat cgccagatgt tgctcatccg tcggtttgaa gaaaagtcgg cggagatgta
    40561 tgcactcgcc aagattgccg gatttctgca tctctacatc ggagaagagg cgattgctgt
    40621 cggtgccatc gctgcgctca gaccggacga ttatgtcatc agtgcctatc gcgaccacgg
    40681 acactgtctg gcgcgtgggt ctgatccggg caaggtgatg gccgaactgt tcggcaaggc
    40741 aaccggattg tgtcagggca agggggggtc gatgcatttg gtggatctcc cgaagcgttt
    40801 tatgggcggt tacgccatcg tgggcggcca tattccgctg gcggcggggt tggcctttgc
    40861 ctcgaagtac cagaagcagg acctggtgac agtctgtttc ttcggggaag gtgcggtgcc
    40921 gagcggccag gcgcatgaag cgtttaatct cgcggcccta tggaaactgc cagtgatttt
    40981 catttgtgag aacaatcgat atggcatggg cacgccggtg caacgggccg tggccttgta
    41041 tgagaatgtg gcggaagccg cgcgttccta tgggattacg gctgagcgcg tcgatggcat
    41101 ggatgtgttg gccgtgcgtg gtttgatgtg caaggtggtg gatcaggttc gagcagggca
    41161 gggaccgtat ttcattgaag ccatgacgta tcggttcatg gggcactcga tggccgatcc
    41221 gtcccatggc cactaccgga gcaaagagga agtggaaaaa tatcgcaagc gcgatccgct
    41281 ggtcgtcttg aaagagacca tggtgagtca ggcgcaatgt agcgaggccg acttcaagca
    41341 actcgagcgg gatatcgctg acgtggtggc ggctgcggtg aaatttgcgg acgacagccc
    41401 gttcccggat cctgcgagcc tgcatgccga tatattggtg gaagactaat ccatgctgct
    41461 gtcttaccgg gaagccctca atcaggccat gcgggaagag atgcggcggg actcgcgtat
    41521 ctttttgatc ggcgaagaag tcgggtatta ccaaggcgcc ttcaaggtca ccaagggatt
    41581 cgtggaggaa ttcgggccgc agcgggtggt ggatacaccc attaccgagg ccgggtttac
    41641 cgggctggca atcggcgcgg ccatggcggg tctccatccg atcgtcgagc tgatgacgat
    41701 gaatttcggc attgtcgcgc tagatcagat cgtgaataac gcggccaaga ttcggtatat
    41761 gtccggcggt cagctctcgg tgccgctggt gattcgcggg ccgggcagcg cggcgcatca
    41821 gttgggagcg cagcactcac agagtttaga ggcgtggttc tgtcacgtgc cgggcttgaa
    41881 ggtggtcgct ccggccacgc cgcacgatgc gaagggtttg ctgaagagcg cgattcgcga
    41941 tcacaacccg gtgatcttca ttgaggctca actgctctat ggcacaaagg gtgaggtcgg
    42001 cgatggtgag tacaccatcc ccctcggcca ggctgaggtg aagcgagaag gcaccgatgt
    42061 gaccatcgtg gcctattcaa aaatgttgct ggtggcgttg gacgcagccg accagttacg
    42121 ccgtgaaggt ctcgatgtgg aggtcatcga tccccgtacg ctcaagcccc tcgaccttga
    42181 gaccatcgtc acctcggtca ccaagacagg gcggctggtg atcgtggagg aagggtggcg
    42241 gttctgtggg ctcggggcgc aaattgctga gagcatctac agcgcggcgt tcgattattt
    42301 ggacgcgccg attgttcggg tcaccggaga ggacgtgccg atcccttata gccggccgtt
    42361 ggaggatgcc gccattccgg acgtgccgcg agtcatcgca gccgtcaaat cactatgtgg
    42421 tgccgcatga ggcatggttg ccgaaggaga caggccggtc gtccaagacc gtccgcttcg
    42481 tggaggagaa tacatggcta gccgcgtcgt catgccgaag ctgaccgaca ccatggagga
    42541 aggcgttctg ctcgcctgga aaaaacatga gggtgatccg gtgcacgcgg gcgaagtgat
    42601 cgccgagatt gaaaccgaca aggccgtcat ggatctggaa gccttcgctc ccggcgtctt
    42661 gcggaaagtc ctggtgcatg atggcgaaac cgtgcaatcg ggcaagctga tcgccgtcat
    42721 cgcggaagct gatgaagaca ttgcttcggc cttgtcggat ggcgtgacgg ctgcagcatc
    42781 gaccggtact ggaccaaagt ccgcaccctc cacagacgcc gccgcatctg tgtcgtcagg
    42841 tcgccctcga ggagaacggg tgctggcctc tccgcgtgcg aaagctctgg cggctgaacg
    42901 gggtgttgag ctctcgaccc tcaccggcac cggtccgggt ggccgcattg tcgaagacga
    42961 tgtgaccgcc gcatccgcct cggtcgccca tgccttgccc gccgggacag atcagccgct
    43021 gagtcagatg cgcaaggcca ttgccagatc gacggtgcaa agcaaggctc cggttccgca
    43081 tttttatctc acggtcgaca tcgatatgga acaggccgaa cgggtgcgcg acgagtttaa
    43141 gcagagccgt cagccccatc cgtcgatcac cgacgtgctc atcaaggctg cggcattggc
    43201 gctcaagcgg catccggaga tcaacgtctc gtttacgggt gaggccatcc ggcggtatac
    43261 ccagatcgat atcggcgtgg cggtcggtat ggacgatggc ttgatcacgc cggtcgtgag
    43321 aaattgcggg gggaagacgt tggccgaaat ttctgtagag acgaagcagt tgatcgagcg
    43381 agccaggcac aagcgattgt cgccgcagga atacacgggg gcgacctttg ccatctccaa
    43441 tctgggcatg ttcgacgtgg atcagtttat cgcgctgctc atgccgccgc aggcggcttc
    43501 catcgcggtg ggcgcgatcc gcgatgtgcc ggtcgtgtcc aggggagcgg tggtcgccgg
    43561 acggcggatg aaggtcacgc tctcgtgtga tcaccgtgcg ttggacggac tgatgggcgc
    43621 gcagttcctc aaagagttta agcgagtgct ggagcatccg gaggaattgc tcacgccggt
    43681 ggtgacgcca tgagcttcat tcaaatcgat tcacgccaca ccgctgagcc gcaggagccg
    43741 tcacggtcgc aatcccgccg cctgccccct tggttcaaag tccggctgca aaccggtccg
    43801 gactatcatg atattcgcaa caccatggat cggctcaagc tgcacaccat ctgcgaagag
    43861 gcgcgctgcc cgaatgtgtg ggaatgttgg aacgcgcgga ccgcgacatt tctcattctc
    43921 ggcgacatct gcacgcgacg ctgccactac tgctctgtgg ccaccgggcg gccgcatgcc
    43981 gtcgatcacg aggaaccgct gcgcgtggca gaagcgattc acgcgctcaa tttgcgccat
    44041 gccgtcatca cctcggtcaa tcgcgacgaa cttgaggatg gcggggcatc ggtctttgcg
    44101 gagacgatcc gccatatccg gcggttgata cccagttgca cgattgaggt cctcattccc
    44161 gacttcgaag gcaatgaggc ggcgctggcg gcggtggcgg ctgaacggcc ggatatcctg
    44221 aaccacaata ttgaaaccgt gagacggctg ttcccatcga ttcggcccca aggcaaatat
    44281 cagcgatcaa tcgagttgct gggacgagcc aaacagatgg ggatgacgac caagtcggga
    44341 ttgatcgtcg gcatgggcga atcgacggac gaagcccgcg aggtcatgcg tgacctccgt
    44401 tcagtggagt gcgacatcat gaccattggc caatatctcc aaccgaccaa agagcatctc
    44461 gccgtcgcgc gcttttacca tcccgacgaa ttcgctgcgt tgaaggaaga aggccttgcc
    44521 ctcggcttcc gccacgttga atctggcccg ctggttcgta gttcctacca cgccgaacaa
    44581 caagtgtcag gacgttaaaa aatctccggc tcttcctttg tcccagacgg cccagcctac
    44641 aaccgtgtcg aacagtctgc catctcgttg tgagctgccc ctattcatag tgcagcgctt
    44701 cgatcggatt catcctcgag gccttgttgg ccggatagag gccgaaaaag atgccgacgg
    44761 ccagtgagaa caggaatgcg accgctatgg attgggaaga aatgatggtg ggccagccga
    44821 tcaggtgtgt caggagacgc gctccgagga tgccgaagac aacaccggct acaccaccga
    44881 tcgcagtgag gatgatcgct tcgaccagaa attgcagcag aatgtgcgcg cgtttggctc
    44941 cgaccgccat gcgaatgccg atctccttcg tgcgctccgt caccgatacg agcaggatat
    45001 tcatgatccc gatgccgccg accaccagcg agatggaggc gatgctcagg agcatcatca
    45061 tcatggtgcg gctcgctccg gccaccgttt tagccacgtc ttccatcgtg cggatggtga
    45121 agtcgttttc ctccgcggga tgaatccgat gtctcacgcg cagtagttcg gtaatttcct
    45181 ccaccgctgt cggaacgctg tagcgatgtt gggtggcgac catgatgatg ccgaccgttc
    45241 ccaggaactt ggtgcccaga acctttcgtt ccgcggtggt gaacgggagg atgacgatgt
    45301 cgtcctggtc ctgccctgag agggattgcc cctttgacga gaggacgcca atcacgcgca
    45361 acgggacgtt cttgatccgg atctgcgcgc cgatgatctc ttcaccacgc tcgaacaagt
    45421 tatccgcgac tgttttcccc aagaccacca ccttggcggc ggcgtcttca tcggcctgcg
    45481 tgaaaaaact gccattggcc accggccaat tgcgaaggtc ggtaaaggcg gtcgtcgtgc
    45541 cgatggcaat cgtgttccag ttcttgtgct cgtgaatggc ctgcaacacc gaacgtgagg
    45601 cataggtgac gaggccgatg tcgccgacgc gtttttcaat gtcccgggcg tcgtcgactg
    45661 tcagtgtcga gacgctgccg agtccgccgc gcactccgcc tgtcgtggta gcgccgggga
    45721 tgatgatcag cacgttcgtg ccgaggctgg cgatttcgcg ttgcaccgag gcggtggcgc
    45781 cctggccgag actcaccatg ccgacgacgg cgccgactcc gatgatgatg ccgagcatgg
    45841 tcaggcctgc gcggagggga ttgcgccgga gcacgcggag ggcggactga atcgtcaacc
    45901 aaagaaaact catgaggaat gtgtcaccgc atcttcggcc cgaactcaaa tcccggcggc
    45961 aatttctcct gagcggtatc ttcggcggtt gcgaggccca ctatggcgct ctccccttcg
    46021 tggacatcgc tcgacgtgat gtctgtatac aatgaatccg caatgccgag gctgaccggg
    46081 accgatcgca cgtgccccga cgcgtccagt acccagagct gaggagtttt acgatccacg
    46141 gctacaccgg gcatgcggaa tcgcagtgcc gcgttcggtg cgcggagcgc atcttcattg
    46201 cgtgcggtca caatggtgat gttggcggtc atgcctggtt taagtttgag gtcagggttg
    46261 tcgacagaaa tgatcacatc gtaggtcacg acattctgaa tgctgacggg cgcgtttcgg
    46321 acctgtgtga cgatgccgtg gaatgattct ctgggatagg catcgacccg gaagtccgcg
    46381 gatttgcctt cagtgacgcc gccgatatcc gattcgctga cgttggcaat aacctgcatg
    46441 tgagtgaggt cctgcgccag gacgaagagc gtgggtgttt gaaaactcgc cacaacggtc
    46501 tgcccttctt cgacattgcg cgagacgacc gtgccgttca ccggagaaaa aatagtggtg
    46561 tacccgaggt ccaattcggc ggacgccagt gtggccttgg cctggtcgac ctgcgcatca
    46621 gacacatcca cctgggcctc ggcgtcgcgc gcattggtac gtgccagatc caggtccgct
    46681 tgggcgacga actgctcctt cctcagcgcg gccatgcgat tcagttccaa cgttttttgc
    46741 acgagcatgt tccgtgcttt ggcgagaccg gcctgcgcgc ttttgagcgc ggccttagcc
    46801 tggctcacct tggcttgata aggtttttga tcaatggacg cgaggatctg gccctcgcgc
    46861 acgaccgagt taaagtcggc atagagtttg gcgatctttc ctgagacctg gctgccgacc
    46921 tgcacggaga taaccggatt gacggctccc gttgcggtga cgagtgcggt gatcggcccg
    46981 cgatcgatcg tcacggtttt gtagtgacca ttaaccggat cattggacca ccagtgccag
    47041 agcgcagcag ccagaagggt gaggacgcct accatcccaa tgacaatcgg cgcccgacgg
    47101 cggtggcgag gaagcgtctg ttggctgatg ctcgccgggg cacgaccatg gccttgacct
    47161 tgcactacgg gaaccgcgtt cacgatgacg ggcgtgggat gggcggctcg tggaggatcg
    47221 atgtccgaat gatctgaggt catgtgttcc cttccgtttc tctcggggaa gacgcaagag
    47281 aagtgccgtt tcgtttccgg ctgaaaaatg tgatgagtcg gaactggcgc tgctgagggc
    47341 cgccggctcg tcatcgatgg tggggcattt tgcaacaggt tgctggagga gcctgcggca
    47401 ggacgcccct tgaccgcata ggattgtggc tattagtctg tgcccgcaga tatccgggag
    47461 gctggtgttg cgagcacatg cgagtgaggg acgaggtatg accgaggaat ggggcgcggt
    47521 attggtggta gatgacgacg ccgacatgcg gagtctcctc tgcgatgtgc tgcaggggcg
    47581 cgggcatcaa tgcacgggcg tcggcggcgg cgacgaggcg ctcaaggaat tgagcgagga
    47641 agattatgcc gtcgtgctca ccgatctgcg catgaaaggc atgcaaggca cggaactgct
    47701 cgcggaaatc aaacagcggt atcctgatat cggagtgatc ttgatgacgg ccttcggaac
    47761 ggtggagacc gcggtggacg cgatgcgcca tggggcgagc gactatctca ccaagccggt
    47821 gaagaccgaa gagttggttc gtgtggtaga gcgcgcggtt cgtgaagcat cgcttcggcg
    47881 cgaggtgagt cgtctgcgga aggaagtgca caaggagtac agcttccatc agatcctcgg
    47941 caagagcaaa ccgatgcagg ccgtcttcga tttgattcga cgggtggccg acagtcccac
    48001 gaacgtgctg atcaccggag agagcggcac cgggaaggag ttggtggcca aggcgattca
    48061 ctacaacagc gaccggcggg atgcgccgtt tgtgccggtc aattgcgcgg cgattccgga
    48121 acagctcctg gaaagcgaat tgttcggcca tatgcgaggc tcgttcaccg atgccaagat
    48181 ggataagcgc ggattattcg aggaagcgca gaagggcacg ctgttcctcg atgaaatcag
    48241 cgagttgccg ctcatgttgc aggcgaagtt actgcgggcc attcaagagc gggaaatccg
    48301 gcgtgtcggc gcgaccaagt ccattcctgt cgatgtccgg attattgcgg cgaccaactt
    48361 gaacctcgtc gatgaagtga agaacaagcg attccgtgaa gatttttatt accgactgaa
    48421 tgtcatcgag ttgcgactgc cgccgttgcg ggaacggcgg gaagatattc cgatcctggt
    48481 cgatgcgttt ttgaagaagt gcgccgccgc tcgcggcaga caggtcaagg ggctcagcga
    48541 gccggccctt gccatgctgg ccgactacgc ctggccgggc aatgtccggg aactggagaa
    48601 tgtcatcgag cgcgcggtga cattgagcca tggggacatg atcggcgcgg aagacttgcc
    48661 cattcaggtt cagggggcgc gaggagatcg gcggattctc gacgaagcgg ctgagcgaac
    48721 cttgccgctc catgaagtgg agaaagaata tatcgtgaaa atcctggaga agacgggcgg
    48781 caataaatat caagccgcgc atgtgctggg tatcgatcgc aagaccctct accgcaagct
    48841 cgccgagatc gagggcaaac cacactcaga cgcgtagatt ctaggtctca ccgtcgtgcg
    48901 gggagccatc gccgtgcacc ctttgtcgca aaaatccctg cttcttgaat atgtcggtcc
    48961 tgcgctcttg accctcggaa tatttctcct ggatctatct gtgtcggccg aatatacggt
    49021 ctggttgctg tatgcgattc ccctggcgct gacggcggca aactcccgac caggcgcgtc
    49081 cctctatggc gcgggattgg tctcgttgtt agtggtgatt ggcggggtcg cgtcacccac
    49141 gggcgttctg ccggtttcct tttgggtaaa tcggttcttg ggcatcgggt tcatggcagc
    49201 catcagcttc ctgttcgcga accgtcggaa tcattccgtg ccgacggttc cgccaactat
    49261 gcctgagccg gcgcacaagg agtcgacacc aaccgagact gtgggggcga tcgcccatgc
    49321 agaagcggcc gtggagggag cagtggccgg acaacgccgg gccgaagcgg aactgcatcg
    49381 cgacaaatta cgcttcgaag ggattgtgca atcggccatg gatgccatca tcacggtcga
    49441 tgaaagccag aagatcgtcc tgttcaacca ggcggcggag aacatgttcc agtggagcgc
    49501 ggaagacatg ctggggcggc cgttggaccg cctgatgcct gagcggtttc gtagcgcgca
    49561 tgcggaacat atccgggagt tcggccggtc gggcatcacc acgcgccaaa tgggagcatt
    49621 gggggtgatc atggggattc gctccaccgg cgaagagttt cccatcgagg cggccatttc
    49681 tcaaatcggc gtggcgggga cgcggtacta cacggtcatt cttcgcgaca ttacggtacg
    49741 gaaacggctg gagcaggaat tggcggaacg cgaagccctc ctgcgagcca tcatcgaaac
    49801 ggagccggag tgcgtgaagg tgctagccct tgatggcacg gtgcgaacca tgaatgctgc
    49861 gggactggcc atgattgagg cagccagcaa tgcggagatc gtcggcaaag acatctgtca
    49921 tctggtcgcc gttgaatcgc gaagcgtctt tcgggacatt gtccgtaaag ccggggaggg
    49981 agaggcggga cgtttggaat tccggatcgt cggcctccag ggcacgccgc gctggttgga
    50041 cacccatgtg gtgccccttc gagcacctga cggaagcatc acggcggtgt taggggtgac
    50101 gcgcgatgtc accgaatgga aaaaggccga gcaactgctg cgccaaagcg aggaacggta
    50161 tcggcggctt ctcggggtct tgcctgacgc agtcctcgtc gtccgtgata atcgggtgtt
    50221 cttcgccaac gagcaggggc tacgcttgtt cggcgccacc cgggcggagg accttcaggg
    50281 gaaatggatc tatgacttga ttcaccgcga ttatcacgag gcggtcgcgg agcgtttccg
    50341 gcatctgctc gcagggggtg gcgcagtacc ggaggcggag gaacggatcc tgcgcctgga
    50401 cggagcgatg gtggatgtgg ccgtccgagc ggcgcagttt agggatgagg acggagcggg
    50461 catcctggtg gtgttgcggg acacgacaat gcgcaacgtg gggcagcaac gactgaagga
    50521 aagcgaggaa cggcttcaaa gtttactgga atccatggag gatgtgattt ggtcatcttc
    50581 gttggatttg acgacgttgc actatgtgag tccctccgtg gggcagatct atggaagggc
    50641 ggcggaagaa tttgtcgcca ggccgtcctt atggatggag gtcgtgcatg ccgaggaccg
    50701 tgcattggct gaacaagcct tgcgggacct tcaggctatc ggcgagtacg atgtcgagta
    50761 ccggatcctg aggcgggacg gtgaggtgcg gtgggtgcat gatcgcggtc gcatcatcaa
    50821 ggatgaaacc ggtcatcccg tgcggatcga tggcatcgcc agcgacatta ctgaacgaaa
    50881 acggctgcaa gcgcagcttc ggcggaccga gcgtgtggcc gaactcggga ccgtcgcatc
    50941 cggcatggcg cacgagatcg gaaccccgat gaacgtcatt ctcggtcggg ccgaatatct
    51001 catggagcgg agcaaggagg agtcggtcaa aaagggacta caaacgatca tcagccaagt
    51061 cgaacggatc acccgcgtga tgaaccagct tctcgctttt gcgcggcggc ggccggtcga
    51121 acatcgtgcg ctcgacctcc gtcagaccat cgaagataat ctggaaattt tccaggaacg
    51181 cttgtctcac agtcacatta cggtggaaac ctcatttgcc gacgattgtc cgtgcgtcca
    51241 tgcggacgcc gatcagatga gccaggtgct gatcaatctg gtcatgaatg ccatccacgc
    51301 catgccggag gggggaacgt tgcgcgtcat gttggcgcct atgccggatc gcggtatggt
    51361 cacactcacg gttgccgata ccggacacgg aatgccgcag gaggtgatcg caaaaatctt
    51421 cgatcccttc ttcacgacca aggagtttgg aaaggggacc ggcttgggtt tgacggtggt
    51481 cagaggcatt atcgaagagc atagcggaac catacaggtg gaaagccagc caggagcagg
    51541 cacgcagttc accatccgct tgccgattca tgtcgagccg agccgcaaat cgtagcccct
    51601 ctcgttttca actcgattcc cagagcggaa ccgcaagcat aacggtcgtc gctgtagcgc
    51661 ttcggggcaa cctatcggag aatgtggggt ctttctcacc aacgctacgg aagcttccct
    51721 cctgtttgga aatgcctgca ctgcagaagc tccaatgaat acaggatctt agcccgtgtc
    51781 aggtcgacat gcgcatggca tacactttgc gatggctgct ttgtcatgag tgtcgcgtac
    51841 tggagcctca acgtgaacga gacaacccca gccgtgctac tggtggtgga agacgataag
    51901 gacatgcgga gcctgctgtg cgatgagtta tggggggaag ggtatcaatt gcgggaagcc
    51961 agcaacgggg ttgaggggtt ggaggctgtg atgcgggccg cgccggatct gatcgtcacg
    52021 gatctgaaaa tgccctctgg gggcttcgat tatgtgcatc gactgagaga ctgcgtccct
    52081 gcctgtccca tcattgtgat gacggcgttc ggggatgcgc ggaccaaaga agaagcgatg
    52141 aaaagcggcg ccacggccta tttcgacaag ccggtgaggc tgtcggaatt aaaggctacg
    52201 gtgaagcggc ttttgcaccg ttccgactcc cttccggagg agccgtcgtt tcactgaata
    52261 cccgcgccgg acgggcgctt tcggctggtc gcccccaacc ggggagggtt gtcttcgacc
    52321 cataagccgt agtacatata gggccttctc tgtgaagaaa ggcccctgct tgattccctc
    52381 tgatagaagg agtgatgaga tggccggatc tcatgcacca gcaacacctc tttctccttt
    52441 cgccgatccc atccttgccg ctcgtttgga agcgttgaga cagttagctg acggcctgac
    52501 cgaccgcgtt gccgtcatgg accgggatct taatgtggtc tatgccaatg atgcggcctg
    52561 gggaaagtcc gggaagccgc cagcgggagt caagccggcc aaatgctatg aatcattcgt
    52621 tcaaaagcaa gatccttgcg ggacatgccc cgctgagtct gtttttgtga cgggtgaagt
    52681 gctgaccgta tcctgcaata catcaggtga tgggactgcc tgtggtatgc accaggcttt
    52741 tccgcttatt tcctcggcgg gagaggtggg ttctattctt gtcttgtttc acaagcggtc
    52801 tgatccgccg gctattccag cagcgctccc ccagggcaag ctgggcgcat cacgcccgga
    52861 acggacagat tcgagattgg gcgatttagt cggtgcgagt ccggcgatga atcagctttt
    52921 tgagatgatt cggttggtgg ccgacagttc ggcgacggtg ttaatccaag gcgaaagcgg
    52981 cacgggaaaa gagctcgttg ccaggaccat ccaccaaacc agctatcgtc gtgacaaacc
    53041 ctttgtggtg gtggattgcg gtgcactgcc ggagaccttg ctggagagtg aactcttcgg
    53101 ccacgtcaaa ggtgcgttca ccggtgccgc cggcgccaag cccggcttgt ttgaagaagc
    53161 cgacggtgga accattttcc tggatgagat tgcggacacg tccccgacat ttcaggcgaa
    53221 gttgctacgg gttctgcagg aaggcgaaat taaacgagtc ggcggtaccc aacccatcaa
    53281 gatcgacgtc cgtgtcattt cggccaccaa taaggatttg accgacctgg tcaaggccaa
    53341 ggcgttccgc caagatctct actatcgttt ggcggtgttg ccggtgcact tgcctccgct
    53401 acgggaacga cgagcggata ttcccctgct ggtgaccaag ttcatcgcgg attcctgcca
    53461 ccgacatcgc caggagcttc gtcaggtgag cgaagaggcc atgcgtgcct tgacggctgc
    53521 gccctggccg ggaaacgtgc gtgagttaca gcactatatc gaacgtgcgg tcgtcaccac
    53581 gacccattca gagttgacct gtacggacct ggtgtcatta aaattccacc tagaacctgc
    53641 cgatctgcgt acggtcgggc gggaggcggc caagcaggcc gaacgcgcca gaattcttca
    53701 ggcgttgcag cagaccgccg ggaatcgaat gaaggccgcc cgtgtcctaa agatcagccg
    53761 agccagcctc tataacaaac tccacgattt cggtatccaa tagagtcagg caactgagtt
    53821 agctgcccct tctcttcttg gtcacatttc ggccattcca acctttcaaa ttcccaacag
    53881 aatcagcccc atgctcttcg tctacgtgtc tcttgatgca tccattgccc cgtatcgcca
    53941 cagaatcgag cgatctgtcg tctagcgaat tggacagtct aagtagttgt aatttcattg
    54001 ttcatcatcc tgtggcgctc acctgtctaa acgtctagaa aaactgtagc gtttcacgcc
    54061 gcttccgatt gtctgacgag agttcataac ctattaataa aactcggtat ttatcgcgtt
    54121 ccaggggtat ccctagggag ggcacatcaa ttgcgatgtt gggcagtgct ggtgaaggtg
    54181 cgggtgatta ggaccggcct ccgaatgacc tgctccctcg aactttgagg acatccttca
    54241 ccagcccttt ttccctttgg agcgtttcgt ggagggagaa aggcgaggcc ggaagagccg
    54301 gggtcctgcc tgacggaggc cggtcacacg accacgtttt ttaaccactc ccgcggaagg
    54361 gcgcgggatt atttaaggag ggaggccgac atatgaggtc ttcagtgcag tcaaaacgca
    54421 ggcgcatcag tcgcaaagcg atgctgagcc tgcaggcggc gctgctctcg ggcagtttgc
    54481 tcgcggcgcc gtgggtgttt gcggccgggc cgggcgatca gtcgcatgaa ggacatgcga
    54541 cgccggtggc catgccgggc tggacccaga ccctgaaggg tcagaccgtg gtggaaaacg
    54601 ccatggaggg tcgggctggc aatgcagaga agatggagat gcagcaccat cgcctgatgg
    54661 agaagttgga gaatcaggcg cagacggatg cgaaggcgca gcaaacctcc ggtgcattca
    54721 acgaaatgtc gatgatgcac cagtatatgg gacaggacgg cagcagcttc ctgctcgcct
    54781 cagattcctc taagggcgaa ccggtgatga cctctggcgg gaagtgccca gccaacgcgc
    54841 cggtcaagaa gtacgacgtt tcgatgatca acatcgaaat cagcttgaat cggtggttgg
    54901 acttctatcc cgggtatatg tacgtccata ccgcggatat cgacaaggct cgcgccgagg
    54961 aagcgaagaa caaggaggcg cgcgaaaagg acggctttga tcctggtgcg gtcacgaccg
    55021 gaaaccaggg cgatgtcatt cagccgctgg tccttcgtgc gaaccagggt gactgcgtca
    55081 agatgacgct gcgcaatcag atggaaggcg aggatggcag cttggtcatc caggcctcca
    55141 gcatgattgt gagctcgacg ggtaagcctg cgacgacgac gaacccggat accgtggtgt
    55201 ccccgggcaa ggcgcaggag ttcgaatggt atatccatcc gaacatgcag gaaggtgtgc
    55261 gtcagttcca ctcctggagc catgatcgcg agttgacggt gctgggtctc ttcggcgcct
    55321 tcatcgtcga gcccaagggc tccaagtacc tcgatccgct cggcacgggc gctgcgcctg
    55381 ataccaacag cggatggcag gtcatgatcg acaacggatc cgggccggac ttccgcgagt
    55441 tcgtgttgtt ctatcacgag atcggcgacg aagccttccg gccgctgaac aagaagggtg
    55501 acttcttgcc gcagcgcgat ccgctgaccg atgcctatcg tccgggtggc cgcgcgatca
    55561 actatcgcag cgagccgttc ggtatcgacc agatgcattt gcagcacgag tatttcggtt
    55621 tcgaagatga atcgctggcc tacagcgcgt acaccttcgg tgacccaccc accacggtgg
    55681 ctcgcggtta cctcggtgac ccggtcaagt ggcggttggt ccatggtgga tcggaagtgt
    55741 tccattccca ccatccgcac agcggaacca tccgttggca gcgcagcccg cgtgccgacc
    55801 aggaagagca ttggtataag ggtcaggacg gaccggtgaa atatccggtg gtccgcacga
    55861 agtcggatcg agtcgacgtg gaagtcgtcg gtccctctga ggcccttgac ctggagccgg
    55921 agtgcggcgg cggtggctgt cagcacctgg ccggcgaatt cctctatcac tgccacgtcg
    55981 cgcaccacta tgtggcgggc atgtgggggt acggccggtt ctacaatacc ttgcaggttg
    56041 gcaatgcgca caccgacacg atgccggata tgcgtgaatt gccggatcgt ttgggccgca
    56101 tgaaacaggg cgtctcgtcc gataagttga tcggcaccac ggtcgactgg ttcggcaaga
    56161 ccttcaagat cgtcgacaag agccagaaga cgaactggaa gtctgatcct gtgatcgtga
    56221 acatcaagga ttgggtcgag atgtttgtgc cagcccaggg tcaaccgggc cacacgaacg
    56281 atgagaaggg ccagatcatg gcctacgatt cgacggtgtg ggactggaag tgggacggca
    56341 acatcgcccg cggtgagcgg gaaagcaccg ctccgaatcc gaagtatgca tgggccgcca
    56401 agtgggatga cagcaccaga ccggcgatcc tgttcgatcc gacgacggga aaaatggcct
    56461 ggccgttgtt caagccgcac ttcggcaagc gcgtgccgtt ctctgcgaac cacagcggtg
    56521 cgccatggtt ggagccgatt caccaggatg caaacggtga gcggacctcc gagccggcgt
    56581 tgccgggcga acagggtcgt tggagcttgt gccctgataa cgccaatcgg aagcattaca
    56641 acattcactt tgtccgcatg ccgatcaccc tcgccaagaa gcaagggaaa gagccggcca
    56701 tggtggacaa agatggtctg atctacgtcc ttcacgaaga agagcgtttg accagagcca
    56761 atgacgatct gaaactgccc gccgtcatcc gcgccaacgt gtatgactgc gtggacttgt
    56821 tgttgaccag cgaatgggac gacgacgatt acacgaactt ccagtcgtcc aagatcaaca
    56881 tccatcccca cttcttccag ttcgacaccg gaaattccga cggtgtcatc tccgggatgg
    56941 agtacgaaat gtcggtccgg ccgttcacca tgtgggggaa gaagaccaag catggtttgc
    57001 cggctccgat ggttgcgaag ttgaccggcg aagcaaaagc cggtgcgacc tccattaaga
    57061 tccagatggc gcccggcgcc accgcgttcc acgtcaacac cgagttgatg gtgggcatgg
    57121 attgtttgga gaagggcaac gatcctgcag cgtcactgcc gcgggataag agctgtcaag
    57181 aagtcgcccg ggtcaaggaa atcaagggcg atgtcgtcac gttcttcaag ccgctgaaaa
    57241 accaccaccc agcgggcgat ctcgtgtcgc cggagtttgt gcgttatcgg tggtgggtgg
    57301 acgtggatat gggtacggtg ttctggcatg accacgcgtt cggcgcgacg acctggcccc
    57361 atggcgggtt cggcgtgacg atcgtggagc catacggatc cacctatcat gatccgaaga
    57421 acggcaagct ggtgtacagc gggccggtcg cggacattca cagcaacgag ccgatcggag
    57481 ccggtgtcag cggcagcttc cgtgagttga tggtttccat ccacgacacg gttccgcaca
    57541 ccgtcaacgt catcgaagcc ggcaatcctc ccggacagcc gatcgaagtg gcgttggaag
    57601 caggaaagac ggtgtccttc cagatgccgg acaagatcct gaatgcgccg aacaagtaca
    57661 tcaacggcgg tacgcatacg accggtagcg gtttcaactt ccgcgcggct ccgtttgcac
    57721 agcgcctgtc caacaatccg gacacctcga agctgttcag cagcgcgatc cacggtgatc
    57781 cgggcacgcc gctcctgcgg gcgtacacgg gcgacaccat ggtgttccgc ctgttgcatc
    57841 agctgatgaa cgaatcccac gtctggacga tcgccggaca taccttcctc acggagcggt
    57901 atgccccgga tgccaatcgg aagaactcga ttcacgtcgg gatcgccgaa cggtatgacc
    57961 tcgtgaccaa ggcgggtgga ttccagggta tgccgggtga ctacatccac ttcaacggca
    58021 gaacgtcaca cttcgccgaa ggtggatggg gcatcatccg cgtgctcgat aaggagactg
    58081 cggacttgaa gccgttgccc agggggacca atcctctcgg gatcccagcg actcccaatt
    58141 ccgtgtgtcc gtcggatgcc ccggtgaaga gcttcaacgt cgtggcgttg gatcgtccga
    58201 tgaagctcaa tccgaaggcc ccggatgcga tcgaggtgga cttcgagcgg aagattgaaa
    58261 tgaccatgcc ggaaggcaag atcttcgcgc tggaggaaga agccgcgacc gtggcgggca
    58321 acgtgatgcc gaatccgctc acgctgcgcg cgaatctggg cgattgcatc aaggtgaatc
    58381 tgaagaacaa gatgaaggcg agccgggcgt cgttctttgc gccgggtctg gccttcgatc
    58441 cgaaagacag ccagggcttg aacgtcggca acaatgccgg cgaccagacc gtggctccgg
    58501 gcgagagccg cacctatacc tactacgcgc atcctgcaaa caaggagacg acgtcgttgg
    58561 tctgggacgg tggaaacatc gtcgtcaatc cgcgcaacgg attgtacggc gcgatcgtca
    58621 tcggtccgag gggatcgcag tatcgtgatc cagtcaccgg tgccgatgtg tcacagaaga
    58681 acacatggcg ggccgacgtc atcgtcgaca ccaccttgtc tgaaaatgcc gggaagcgga
    58741 actaccgtga cgtggccctc ttcttccaag atgaggacaa catcatcgga accgccttca
    58801 tgccctatgt gcagaacgtg gccggtttga cgtcggtgaa ctaccgcgct gaaccgtaca
    58861 agttccgcga agaacaaggg tgctccttgg gcaaaatctt ccagccttgc gcggtcgata
    58921 agcccgaaga tcccgtcacg cctttgatcg aggcgcacgc gggtgacgcg ctccgcatcc
    58981 atgtgatcgg agcgaacagc gagcagaacg gcatgttcag cgtcgaaggc cacgagtggc
    59041 cgatcgagcc gtacatggcg ggtgccgaca tgatcagcgt ggtggagtat gccgggtctg
    59101 agaccctcga tatcttcatc cgtggcggtg cgggcggacc gtatcgtcag gtgggagatt
    59161 tcgtgtggtc caatgcacgg ctgccctaca cgcagtccgg acagtggggc tatctgcgcg
    59221 tgttgccgac cggcgattcg cggattcagc ctctgagcgc aagtggtgcg ggtgcacgtc
    59281 aagccgaagt gctgcccgag ccgcaggcga ttccgactgc catgaagtaa gcaggcacgt
    59341 catggcgtaa caccggatgg gggagctgcc tgtatcacgc agctccccca tctgcgtttt
    59401 gagtatcgaa gaaggaattc agtgatgggt tccctgtatg attacgatga gaggtcgagc
    59461 atacaacaac ccatcacaga ggaggccggt tgtgacattg ggtattacag caacgaacga
    59521 gcagcaacac atgacgcatc gtatttctgg ttggaaggtc ggcttggcgg cgatggctct
    59581 cggagggctc ttagcaagcc atcccgcgtc agcctatgaa gaaatcactg tgtcggatgg
    59641 cgggacgttg tcgggagtcg tcaagctcga gggaaccgtt ccgaaaccga aagggtacaa
    59701 cctcaccacg ttgcccgatc cctattattg cggtcgcatt tccgacggag aaggctggcg
    59761 catcctgcag ccgtttaatg tcggccccaa gggagagttt cgcgaagtgg tcgtctatct
    59821 agaggggatc gaaaagggga agtcgtttgc ggagaaagag gtgccgcaaa tcgaagccaa
    59881 ggattgcctg ttcctgccgt ttacaactgt cgtgcgtgac gatcagtctg tgaccgtcgt
    59941 caatatggat ccggtcatgc acgatatcca ggcgtatgaa acgtcgaact tgggcgcgcg
    60001 cgtcttgttc aacgtgccct tgcccatgaa cccgcagcat ccacggaatt tcaaagatcg
    60061 caccgaagcc ggcatgtatc acaaacatat ggccggtccg ccgatgaaag agctggtcaa
    60121 gctcagcaaa ggccgacgga tctttgtgat gcagtgcggc ttccacgcgt acatggaaag
    60181 ctggggcgtg gcgattgcca acccgtattt tgccaagact gacgaagaag gtcggtttac
    60241 catgactgac gtgccacccg gcacgtataa gttggttgtg tggcatccct atatcaggac
    60301 cgccacggaa aagatcgtga cgattgggcc taagggaacc gttgaaacca cgatcggcgt
    60361 tcccgcgccg accgggcggt tgtatgccaa tgaggtcttg gaccacgctt atacccggta
    60421 caacgtgact gaggaagcga agaaagagat cgatcctttg ctccaaaagc aggagcacta
    60481 agttggtcgc gcaaggagca cgtctgatca gcaaggcaat gggtatctgg cgaggtgggt
    60541 gtctccgggc gctgctgggg tgtgttgcca tcgggctgct gcccggcttg gtgacgccca
    60601 gccacgctga ccatgaattg ccgaaaccgc cgccgctctg gtctccgctc gacaatgtgg
    60661 agcggctggc gttgatcgac gtgccgaacg gaatggctga ggtgccggcg ggcccgtttc
    60721 tgatgggaag cgacccgaag tttgatcggg ccgcaggacc gcaggaactc ccccagcacc
    60781 aagtctatgt ggatgcattc agcatcgacc ggtacgaagt cagcaacgtg aactatctgc
    60841 ggtacgtgtt ggcgaccggt gcggcctggc cgcactactg gcgggagcag ccattccccg
    60901 agaagatggc caaacatccg gtgatcggtg tctcctggcg ggaagccgat gcctattgcc
    60961 ggtggcgtgg ggctcggctc ccgactgaag ccgaatggga gaaggctgcg cgcggtgaag
    61021 acggccgcat gtttccctgg ggcaacgagc cggccgggtg gatcaagagc aacatcgctc
    61081 acccggggtc caagcggggg gcgaagtatc cgccactggc caatgtcgat cgctacgaca
    61141 atggtgtgag tccctacggc gtgcatcaat tagccggcaa tgtgagtgaa tgggtttcag
    61201 actggttcga tccggaatat taccggcgac aggaaaatcg caatccccag ggacccgcat
    61261 ccggccagga taaagtgttc cgcggaggat cgtggaatga ggatcccgag gtggcccgtt
    61321 ccgccggacg caattccgga gggctggacc actggagcta tctgacaggg tttcgttgtg
    61381 caacatcggg cgagagtggt cgtcagcttt cgtctatcga tccggctatg ggacgcaata
    61441 cggatcggta attgtgacgg caggacaatg aggaggccat agatgaaacg actgcagaaa
    61501 gggaaaagga aacgaacacg attggcgcag gccctcgtga ttggcgccgc gctctttgcg
    61561 ctggctccga ttgcgcgggc gttggatacg caggacatcg tggtggagtg gaccgaagaa
    61621 gggaaaaaga tcgcgcagga gcgggtggcg aattggaaaa ccaaagacga aatggtcctg
    61681 gttccggccg gtgagttcct catggggagc gataagaaga cagatcgtct cgcttaccgc
    61741 agcgagattc cccaacggtc ggtctactta gatgcattca tgatcggcaa gtacgaagtc
    61801 accgcactgg agtatctcaa gtttgtcctc gccacggacc gtttgccgca gttggactgg
    61861 cgctacgacg gaggcaattt ccaggatacg atggcgcatc atccgatcat gcatgtgagc
    61921 tggtacgacg cagacgccta ttgcaaatgg gcaggcaagc ggttgccgac cgaagctgaa
    61981 tgggagaaag cggcacgcgg cgtggatggc cgactgtttc catggggcag tgagtatgcc
    62041 ggtccgacca gggccaactt cggcagaacc ggattgtccg gcccagtgcg ggatcgcccc
    62101 gaacggttgc tgctgtatcc cccgatcatt tccgtcgaca agtacgagaa tgcgctgagc
    62161 ccctacggac tgtatcaaac catcggcaat gtggccgaat gggtttccga ttggtacgac
    62221 caggattatt acaagactgc accggctcgg aatccgaaag gcccggagac cggcacgcag
    62281 aaagcgttcc gtggtggcgg gtggatggat agcaccacca ccatgcgagc ggccatgcgg
    62341 aacggtaccg atccgaagac taagatcaac tggatgggat tccgctgcgc tcaggatgcc
    62401 aaggaagaca cgggaaccaa ggtttcgatg acgaaggagt aacaggggaa agggcggtga
    62461 gaacgtagtg cttgccgcca caatctctga gccggtcgtc ttctggatgg ggagacgacc
    62521 ggcctccctt ccctccggtt gcgctatgcg gtcgcgtcgg cgcactcatc atcgcgcgca
    62581 aatgaaaaaa tggagcctac cgaagagggt aatcaatccg cctctaagcc ccgagatcac
    62641 caacaagctc accggcaatt acgcagcccg tcgatatcgc ctgccactct cagcagcatg
    62701 tccagcaagg gagtattgac ctgcagtgcg agctatacgg gagaccgatc tccactcgcc
    62761 aggataagta gtcatgcgca gactcctctt gacggtcgat gacagattgc gttatttcct
    62821 acgctgaatt cgtggtgaaa gacggtgctc accttgttgc gagtcatctt cgagagcagg
    62881 gtgtggaagt ggtaacagga atcagccgat cgtgaaacgg aagccctgcc tcatcgaaca
    62941 ttgtgacacc tgcgggcttc gcgagaaggt cgttctgtgc gacatttcag ggagcgatct
    63001 ccaagagttt cagaaaatca aacgcacgct cgagtacgct cctcatcaga ctgtgtttta
    63061 cgaagggcat ctgtgcctgg gcctctactt gctctgctcg ggcaaggtca agcttacgcg
    63121 ttcctcgacc cgaggccgga gacagatcgt ccgcatcctg ggcccggggg agctgatcga
    63181 gaaacacgta ttcggcgatc gggcccttca tgaagtgacg tgcgaaacat tggaaccttc
    63241 tcaagtgtgc gtcatcgata aggaacgcta cctcgccgtc atccacagaa atcctcaact
    63301 ggcaatcaag ctgatccagc tcctcagtaa cgaagtcggg gtcaatatgg atcatctcga
    63361 tcaattcaca ttcaaaaccg cccgtgagcg cctagccggc ttgctcctgg agctcggcga
    63421 ccgattcggc aaaaaaaaga cgatcaagtt cgagtcggtc tcaccctcaa gcgtgaagaa
    63481 gtagctgaga tggccggcat tacggtcgaa accaccatcc ggttgctcgg cgtgtttcgc
    63541 gacgagggag tcctcaccat tgatgggcga accatcacct tgctgaatcc agaccgcctc
    63601 tccagaattg ccgagcgctg atcaccccct ttcccgttgc gtgacccatt gttcgtgaag
    63661 cgcgtcgggg cgacgcgacg ttcaagtttg cagtgctgac agcccacaca caactcggct
    63721 ctcccctcat tgcactcatc agcttctcgt ctctcacgca tcgcctcctg caggcatagg
    63781 tctaatggcc tatccaaaac cttgagataa atcatgcttc cgaccggctg atgccagtgt
    63841 ggcctggtta cctgttcggt acagtgcgca gggcatgacg ctgtcgtatg gctcgcagat
    63901 tggagcttcc gtccatggga ttccggaacg atggtgcctc aaggtagcag gcgaacgagt
    63961 ctcctcgtcg atcctttggc gctgctcaag cgggagcatc acatgattct ggagcgactg
    64021 gcaatgatcg agacggcgat gagtccctgc tgctccggca gcggagcggt gaagcagacg
    64081 aatcgtgaca cgctacggga cctgctcgaa ttttttaccg gcccggtgga tgtgcatttc
    64141 aagcgggagg aaatgctggt ggacaatctc cggagaactc tgggccggaa tcgggaagaa
    64201 aaagagcagt ttcagagctt cgtggaagaa caccaggccc tgaaagatga tgcgacagcg
    64261 gtgatgcaac agttggcgag aaagcggagc gacggccaag acggagtgga gccaaaggcc
    64321 tgcggagggt tgcgagcgct gaccgatgag cttcaccggc tgattcgtcg ctatcgtgag
    64381 cagattgcct gcgaagagcg gctgctgttc gccttggccg agatgcggtt gaccgccgac
    64441 cagagacgtc gaatcagcca acggatgttg caagtgtgaa ctcgtgcatc agcggcaaca
    64501 ggcgtgggac cgatgaggcg cgtgactggc tggtgtgagg ccggcacgag gggcactgag
    64561 gtgttggatg agcgacggag gaagaagacg gggaatcgcc gcgatgttca gaagggtgaa
    64621 tcacatgctt gcgctgctcc tgtctcgggc cggttgggga aagacgggga gccaacccgg
    64681 gcgccggtca ggcgcgtcgg tgacacatca aggaggggat tatgaagcgg gtccggaaag
    64741 tcgtgcagaa cattggaatg tggggtgcgg gggttgcgat cgccgcggtg ctcggtgttg
    64801 ctggtgtgca gccggcatcg gctgaagaag gcacgggcaa catggtgttc ttcaagggcg
    64861 gatttatgaa tttgaaccag gatcgcagcg ggcagatttt tacggatacg gcaggcatcg
    64921 ccggcgccaa tggaggcaac gccggatggt atgcgggggc ggggctggat ctggtcatgt
    64981 cgaaggatgt ctggggcgga atggacaaga cgtgggtgct cggtgaaatc gggctgcaat
    65041 ttaacagcat taattcctcc cgcgtgaata atgccgccgg gagcattgcg accagaacgt
    65101 taactgacac gctggcgacg gatccgcaga aagtgcagct cacgatggtg acgatcgacg
    65161 tcgctccgaa aatcaagttt atggaaggca gcgcgttcag gccatgggtc attccggtcg
    65221 gtctggatat tcatgtcatc agccctccgt cgaatcgcac gcagtacctc gacctgggcg
    65281 tgcagttcgg tgccggtttt gagtatgcgg tctggaaggc gttcaaagtc ggggttgatg
    65341 cccggtacca cctgacggca cgtctgacca acaccaacaa cgattacttc caggttggtc
    65401 cctatgtcgg gatttcgttc taagcacgcg agaggtgagg cgtcttagtg taggatcgga
    65461 acatcagtcg taagccaatg agaatgacgg tgccacaccg gccgttgccg aaagcaggcc
    65521 ggtgtggcat cattccgaat cgagatctcc ggaggccgcg cgttggtgga tatcacgctg
    65581 tatcttcccg acgattcttc cttgccgttt ccgattggcg aacattctcc acagcaggcc
    65641 ttttcgcatc gcatcgaaga aaccggttcc gggctccatc atgtggtgac cttcaccggt
    65701 cttcccgaac acatcgatct ggcgttgcag gcgatcggtg aatccgtccg tattcccgga
    65761 gcgtgggcgg cagttggggg gcaacggatg tccagcctcg tcgccttgtg gaatcggctc
    65821 gactgttaca ggcagagtct gtcatgttcc gatcgggtga gctactgcag gagcaaagcc
    65881 gcgagtgttc gagtcgggtt gggctgcggg ctggcgttct gctcggcgcc gtgccagttt
    65941 ttgtgcccgc tctgcagctt gaaagacact cctcttgcac agtccattgt gcaccccgaa
    66001 acgcaggtct tggtcgtttt gggagaaatt gattggtgcc cgaatctcgc tcgtcctgcc
    66061 gaccggtagt gtggcccgcc ttgcgtcccc gttcatccct ctgtcatggt gccgttgagg
    66121 catagcggaa aaaggaggcc ctgtgtttgc attggtgtgg attcacctct tagcggctgt
    66181 cgtgtgggtt ggcgggatgg tgttcctctc cgtcgtgttg gtccccgtgc tcaaacggga
    66241 cgggggtttt gcgcgccacg ccgcactctt ccgaacggct gcctatcgat tcagagcgct
    66301 ggtgtggggc gccatgggaa ttttggtcgc caccgggtcg gtgctagctg cagggcgagc
    66361 cattcctttg agtgatccgt cgcaatggcc gtctattttt atgacgaaga tcggactcgt
    66421 cgccttcttg ttcacgctca cactgttgca cgatctggtc gtggggccac aggtgcggcg
    66481 cattctcgga accgcagagg cagcgaggag tgcaaaggat cggttgttag tgcggtactc
    66541 cgtgctggta ccgcgcgtgt cgctgctggt ggcgttgctg gtactattgg cggctgtggt
    66601 gttggcccgg acctagcctc gttcattgaa gaatgatcga ggcaacgtac gatcgatggt
    66661 cgagacggcg tctcgtacgg gcacgcttcg gggaaagtct tatcgtgtcg cgggagttgt
    66721 ggagggccgg agtgcgtcaa ccagacgttc ggtttgcgcg tggctcgaga gggccaattt
    66781 catcaccacc ccgcataacg cgaatacgac cgcgttcagc aggaaaaaga cgagcgccag
    66841 tgaaccggtc gtgagtgccc aggcttcgga gtctgcgcga ttgcgcgcta acacccgtgc
    66901 ctcctcgtca cggatctgct ccgtgatgtc atggatcgtg tccatcgtcc gattcagttg
    66961 attctgagtc acgatggatt tggctcccgg tagtccctta gtcctccgga gggcaatggc
    67021 ctgttccatt tcgtccgacc gctggtcgac ttgtgtggca agataggcca cccgatcctg
    67081 ctgcaagtta ttgtccctcg tcaggctgcc gatccgtcgg atctgttcct ggagcatgtc
    67141 gagcgcttct cgatacggtg ccagatagtt atccgacccg gtaatcaggt aaccgcgctg
    67201 atgggtttcc gcccccgaca caatactccg tacacgttcg agttcaatta agacctgttc
    67261 atttcgggtt gcccaattgt ttgaggtttt gaagtgcatc aggctgagga aacaggcggc
    67321 cacgatggac tcgactgcaa gaaatcccac aatcagggca atgatcaggc ctttgccggc
    67381 gaggattcga cgaagccacg tcagatgggt ctcgtacaag atcatcctat agcactaccc
    67441 acccctgccc cctgatgtca acccgacaaa tcccttggtc gggtcggcga tgtgtcaaaa
    67501 gtgtgtcgtc gcagaacagt gggtagcaga cctgcgcttg aagatgaatc gctccagtcc
    67561 cgtcacaact atcagccggt ggatgaatcc attttgtgct tcgtgcctct caatcattga
    67621 tcgcaggcat aacctgtgca aagtcagggc attggtgaag gccgtacgca tggcggctct
    67681 ggcgtctaaa gcccaagcgc aagtctcagc ggcggtccac ttttacggag gtgcatcatg
    67741 gcgagagtgg tcatcatcgg ggcatccatc ggggggttac cagcggccta cgaagcacga
    67801 gaattgttgg cgaagaaaca caaggtgacg gtgatttcca atgtggaatc ctttcacttt
    67861 gtgccatcaa atccttgggt cgcggtcggg tggcgtaccc ggaaggacat tagtttcgcc
    67921 ttgggtccgg tgttggagaa aaaaggggtg gagtatatcc acgcgacagc ggaccggatc
    67981 gagcccgagc aaaaccgggt catcacgtcg aaaggcgaag tcccgtacga ttacctcatc
    68041 atcgcgaccg ggccgaagct caatttcggc gcggtgcccg ggctggggcc aagcggctat
    68101 acgcagtcgg tctgcaacgt ggaccatgcc gaacaggcct ggggcacgta tcaggcattt
    68161 ctcaaagacc caggtcctat cgttgttgga gcggcgcaag gggcatcctg tttcggcccc
    68221 gcctatgaaa tggcctttat tctcgatgcc gaccttcgca agaagaaact ccggaagaaa
    68281 gttccgatct atttcgtgac gccggagccc tatatcggcc acatgggcct cgcgggggtc
    68341 ggcgcgtccc gccggttgat ggaagatgaa tttgccgagc acgcgatgaa gccgatgtgt
    68401 aatacgtcga tcaaagaggt gcagccgggg aagatggtgc tggagggcgg gcaggagatt
    68461 ccgttccgct actcgatgat cattccaccc tttgccggcg tcgacgcggt cgcgggaacg
    68521 ccggggctgt gcaatccgaa agggtttgtg aatatcgatg cctatcaggc gaatcccaag
    68581 tataaaaata tctattccgt cggggtctgt gtggccattc ccccggtgga acagacgcct
    68641 attccgaccg gcgcgcccaa gaccgggtac atgatcgagt cgatggtgtc ggcggccgtg
    68701 cataacatca aggccgatat cgacaacgac acaaaacgtg aaacggcgac gtggaatgcc
    68761 atctgcctgg ccgatatggg tgacaccggt gtggcgtttg tcgccttgcc gcaaatggcg
    68821 ccgcgcaatg tgacttgggc gaagaagggc aagtgggttc atttggcgaa aatcgccctg
    68881 gaaaaatact ttttgcgcaa aatgaaaaaa ggcgtgagcg aaccctatta cgaaaaggtc
    68941 atccttaaag cgatgggtat tgcgaagctg gaagagccgg catgaaggga aaccggtaga
    69001 gtcgtatgat cgagcaggcg ttgcgcgcgg tggtgtccgg ggtgcctatc ggcctgcaat
    69061 tgggtgcgac cggaaccggc ggcgcgattc tcggggtgcc gctgatggtg tatatcgccg
    69121 gcatgtccct gcagcaggcg gcggccatgt cgttgatgat tgtcggggcg tcttccttgg
    69181 taggcgcgtg ggaatatggc cggcaaggga tggtcaaacc gaaagccgcc gcggcattca
    69241 gttggacagg catgatcggt tcgtggggcg gcgcgttcgt ccatcacatg atccgacagg
    69301 aaatattgct ggtgctgttc ggcctgctgt tgctgctggt cagaaccgtc atggcgcgcc
    69361 agcgggaact ggcggctgag catggagcgg atgaaagttg tgccacgctg ttcccgcgca
    69421 cctgctggat gaaggtgggg ggaatcgggt tgatggtggg gacgctgaat ggattgttcg
    69481 gggtcggcgg cggcttcatg gtcgtcccgg ccttgattct ggtgttgggt tttcctgccc
    69541 gtgcggcgat cggcacgtca ctcatgatca tttttatgat ctccatcggc ggcgtggtgg
    69601 gacatatgca gttcggtcat gtcgagtgga gcctgttagg ctacgtgctg ctcggcagcg
    69661 tgaccggcat ggtactgggt gttcgcgcgg atacctgggt gtcccctgtg gtgatgcgtc
    69721 gagtgaccgc ctcgatcacc atctccatcg caggcatatt gatcgtggtc aacctggcga
    69781 agcttgccgg gtggctaggc tgaactctat tgacaggtcg cggattctgt cggactctca
    69841 gaggaaggtg ctcgtggatg ttgttccggg catgaggtgc gggtgccggg ggaacgcatt
    69901 ccgttcaagg cttacatggg ctctacattc ggatgatgcc gtatcagcgt gtccttgtgt
    69961 ccgtcgcatt ccgtgtcgcg ttatggaccg tggcaggata ttccatggtc acaccggagc
    70021 ggacggcgca agccgaatgg tcggcgatcg ccgagcaaaa aaccagctat acaaccgatg
    70081 cgtttcaatt ttcttccgcg cgccggctcc gtttgacgga agatccttct ctgcccacgg
    70141 tcgtctcgac cgacaaacct caggatgtga tctgggagcc cgccctggag gtcatccatt
    70201 ccacgccgac gatgcagggg aaaaacgaat tgtcggtgaa ggcgcaaggg gcgatttaca
    70261 ccaacaatcc cattttcaac cattccgact ttcgcattca ggatcgcttt tcgctcgacc
    70321 aggcgacgtc ggtgctcgtt cgctaccgat acatccccaa tctctttctt ggccccaact
    70381 tcgagcgccg aaccggcaca cgctcggttc aggaagaacg ggtcagttcg catcattggc
    70441 gcgccgaggt cgaacgccgc ttgtccgaca cggtcaccgc gaccctcatc acccgcttcg
    70501 ggatccggcg ctacaacgat gtgtttgcgg aacgcgatac gaacctctgg acggtcggct
    70561 cgcgtgtgga ctatcgcgcg aaggaatggc tgacgctcac gctgtcgtat ctgtacgaac
    70621 gaggtctggc ggatggccac aaggagactc aatttgccga tgacgtgtcc tactatctcc
    70681 acatggtctc ggccggtacg gagttccggt tgcacaagga atgggatctc ggcttgacct
    70741 atatccatcg ccggaaaaca ttcaccagcg gtattgcggg cgacagccat gtcgatcggt
    70801 ttgacgccac gcaccagggg attgcggagc tgcgatacca catctcgacg gctgcgacgg
    70861 ctatgttgtc gtttcagtat ggcaaacgta cctcgaccaa tgtcctgaga gactttcagg
    70921 attcgattgt ctcgatcggt ggtcaatacc ggttttgatg ccgaccgggt ggcatggttg
    70981 ccgctctcgg aggctaagtg actgaataca caatgaactg ccgttgaatc ctttcatcgt
    71041 ccggatactc atacggaatg agaaaggagg cggattatga agttgaacga aatgcttcgg
    71101 ttgatcgctg gtgtgttcgt gttgatcgcg gtggtgttgg gtgcaacggt tcatccctac
    71161 tataattatt tcgcggcgtt tgtcgcggcg aatctcatcc agtcgtcatt caccggctgg
    71221 tgtccgatga tggctctttt gcggaggctt ggtgttcacg agtaacgaca cacgtttcag
    71281 cattggaggc cttgtgctcg ccctgctcct ggcggccggc tgcgggcctt ctcaggaacc
    71341 cggcaagccg gggaacatga ccccgggtac tgcgggacag gagccgatcc aggtgccggt
    71401 gatcgaggtc gcagccagcc aggtcccgat ctcagtcgag gtgacagggc aggtaacagc
    71461 ggtctatcag gcgacgttgg ccagccggat ccagggaatg atcgataacc tgctggtccg
    71521 cgaagggagc gtcgtgcgtc agggacagac tctggtggtg ctcgacaacc gggacgtgca
    71581 ggcggagttg tccagagtga cggcggaact cgacaatgcg aaggcgcatc aagaccggat
    71641 ggaagagctg tatcgcaaag atgcggtatc gaaacaggaa ttggagaatg cgacgcgtgc
    71701 cctcagagtg gctgaagccg gccgcaaggc ggtattggcg caattgagtt acaccatcgt
    71761 caaagcgccg ttcgacggtg tgatcaccga aaaaaaggtg gagcgcgggg aattggcctc
    71821 cccggggcaa ccgctcctga agatggaaga tcccgggcgg cttcggttgg aagccaccgt
    71881 agccgaaagt gatgtgcggg tcgtgtcagt cggctcgttg ctgcccgtcg tgatcgacgc
    71941 cttgggggcg aatccgctca aaggcaccgt gtctcagatt ctccctgccg gtgatccgca
    72001 aacccacacc ttcacgatta aagtcgattt gccacaggca ggcggcctga agtccggcat
    72061 gttcgggcga ctcaaacttg agaaggggat gagtacgacg ctgctcctgc cgaaatcggc
    72121 gttgatcgaa cggggtgaac tggccagtct gttcgttgtc ggaggagacc agacggctcg
    72181 ccttcgatgg gtcaggatcg gccgcacgtt ggaacaaggg gtcgaagttt tgtccggtgt
    72241 cgatgccgga gagcgggtca tcgctgacgg aagcaaaggt cgcgacggcg cgctcgtgca
    72301 ggatatgacc gcggtggcct tgccgcccca ggcgcggtaa tgctgtcgac agggcggtct
    72361 ggtacacagc acaacggatc ttgtctggat gtccatgatg gttcacgaat gacgacccca
    72421 cgcctcggcg ccagcggacg tctcgccgca ctcttcgtcg gcagcaaact gaccccgttg
    72481 atcatcctcg ccagccttct cctcggcctg ttcgcggtac tgttgacgcc tcgcgaagaa
    72541 gaacctcaga tcgtggtgcc catggccgat gtctggctcc cattccccgg ggcctccgcg
    72601 aaggtcgttg aagagcaggt caccaaaccc atcgaacgca agctctccga aatcaaaacg
    72661 gtcgaatatc tctattcgat ttcccgaccc ggcggcgcgc tcatcatcgt gcggttttat
    72721 gtcggtgaac cgatggagca gagcctggtg gatctctatg acaaattgat gtccaaccag
    72781 gatctgttgc cgcctggcgc cgcgccgttt caagtcaaac cgcgtgacgt caatgatgtg
    72841 cccattgtca ccctgacgct gtcgagcgag cgctatacgg atttccagct gcgtcagttg
    72901 ggcgaccagg tgctggagga tgcgaagaag gtgcctggca cggcgagtgg tttcgtggtc
    72961 ggtggccgag gtcgcgagct gcgcgtccag attgatccga cacggttgaa agcctatgga
    73021 ttgacaccct tgcgagtcgc cagcgtcatt cagggggaga atttagcgct gcctgccggc
    73081 cggtttgaga gccgaaacga aagcttcctg gttgagacgg gccggttcat ccgctcgaag
    73141 gacgatcttg acggcgtcgt ggtcggtgtc aatgaacaac ggccggtcta tcttcgtcag
    73201 gtggcggagg tggtcgatgg tccgagcgaa accaatcagt acgtatggtt cggcgaaggg
    73261 gcgcgaagca gttcttcctc ttcgggtgaa acaccagctg tgacggtagc gattgccaaa
    73321 caagccggca ccaatgcggt gaccgtcgcg cagggagtga ttcgaaaagt cgaggaaatg
    73381 aaggggcgat tgattccagc cgacgtgcag gtgaccgtga cgcgcgacta tggagaaacc
    73441 gccgatgaga aagccaacga actgctctgg cacctgttgg tcgccgtggt tgcggtggtg
    73501 gtctttctcg ggctgacgct gggattccgg ccggcattcg tcgtctccat tgccattccc
    73561 ttgaccctgg cactgacgct gttcatttcc atgctgatcg ggtacagcat caaccgcgtc
    73621 acgttgtttg ccttgatctt ttccatcggc attctggtgg atgacgcgat cgtcgtggtg
    73681 gagaatacct atcggcatct cacactccgt ctcctcccgc atcgcgaggc ctcgctcttt
    73741 gcggtcgacg aagtcggcaa tccgacaatt ttagcgacca tgacggttat tgccgctctc
    73801 ctgccgatgg ccttcgtgtc ggggctgatg ggtccgtata tgcgtcccat tcccgtgaac
    73861 gcctcgattg cgatgtttgt gtcgctcctg gtcgcattca tcgtcatccc ctggttctgt
    73921 cagacgtgtt atcgaccggg cgtacacatg gcgggcgtcg atcatgattc gtttgaggag
    73981 ggtcgcagtt atcgactcta tcgccggttg ctggctccgg tgttgtcgca tccggtgatg
    74041 gcttatctgg tgataggcgt cattgggctg ttgttggccg ggtcggtcct gttgttctgg
    74101 acgcgcgacg tggtggtcaa aatgctgccg ttcgacaaca agagcgaatt gcagctggtc
    74161 atcgatatgc cggaaggcac gacgcttgag gaaacggccc gggcgaccaa ggccctgacg
    74221 caatatgtgc gaaccattcc cgaaatgcgc gattaccagg cctatgtcgg tacggcctca
    74281 ccgtttaact tcaacgggtt ggtccgacac tattatctgc gcgagcagcc ccacgaggcc
    74341 gatatacaga tcaatctcgt ggcgaaggat cgacggcagg cacagagcca cgacattgcc
    74401 caacggattc gcccgccggt gcaggacatc gggcggctct atggggcgaa tgtgaaagtg
    74461 gtcgaagtgc cgcctggtcc accggtgcaa tcggtcctcg tcgctgagat ttatggccct
    74521 gaatacgacc gccagatcga gatcgccagg gacgtgcgcc ggatgtttga aaccacggcc
    74581 ggtgtcgtcg atgtcgacga ctacttggaa gcggagcagg tcaagtacgt gtttacggtc
    74641 gaccgtgcca aggcggcctt ggcgggcatt ccgtcagagg aaattgtgcg gaccctccgc
    74701 ctcgcactgg aaggtgccga cgtcggcctg gtgcatatcc cgaaagagcg gcgaccggtg
    74761 caggtcaaac ttcgcctgcc gatcattgag cgaaccggcc tggaacatct cggagaaatc
    74821 gccctgcgaa cgaccgcggg tcatatggtg ccgctttccg aactcatccg cgttgaacag
    74881 accgtccgtg aacaggcgat ctaccataaa aatcagaagc cggtcgtcta cgttatcgcg
    74941 gatgtcggtg gcacaggccc cgacaagggc gagagccctg tctatggcgt gatgggtatc
    75001 ggcaagaaat tggaacagta caggctgccg gaagggtatc gattggaaca acagtatgcc
    75061 gtgcagccct ggtcggaaca gcggttcgcc atgaagtggg acggcgagtg gcacattacc
    75121 tatgaaacgt ttcgggatat gggtctcgcc ttcgcggcgg cgctcgtgct gatttatctg
    75181 ctgatcgtgg cgcagtttca gtcgttcctg acgccggtgg tcatcatggc gcctatcccg
    75241 ctgaccttga tcggcattct tccaggccac tggctcactg gatcctattt cacggcgacc
    75301 tccatgatcg gatttatcgc gctcgccggc atcatcgtgc ggaactcgat tctcctggtg
    75361 gatttcattc agcatcagga agccgaaggc atcgcgctgt tggaggccgt cattcgcgca
    75421 ggagcgattc gtacccgtcc gattctgctg accgcggcgg cgctgatggt cggtgccttc
    75481 gtcattattc tcgatccgat ctttcaaggc ctggcggtgt cactgctgtt cggggttggt
    75541 gcatcgacag tgttgaccct gattgtcatc ccggtgctgt actacctgtg gatcggcgag
    75601 cgccgacctg tctctgcctg tcccttgcct caagctgata acccggcagc gccacgacat
    75661 gccgggggca atcagcctgg ctgaaccgtt cctggaatat ctctccttcc ctgcaatcga
    75721 tccgctccta ttccacaaga cccttcttga agtcctacgt acaccgctcc ccaggaaaaa
    75781 agcttctcaa tcggcgagcg gggaaactat actgtggccc cacgcggcac gccgttgtgc
    75841 cgcaagatct gtgatggatg gaatctctcg tcatgtcgcg aagtggtgag catgtgtcaa
    75901 tgggcagtgt aggtcacgag caggaagcgc cgctgctctc ctggtgtgag gcggatacgc
    75961 gtcggtatgt ccagatgctg gtactggctc ttgtgagcgg gtgggcctat tttgccaatc
    76021 tgcacctctc gttattggaa ggcagcgaag ggctgtatgc gggtatcgcc ggcgagatgg
    76081 ccaggcgggg cgagtttttc gacctcacct atcaaggcat tccctactnn nnnnnnnnnn
    76141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    76201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga gatggccagg cggggcgagt ttttcgacct
    76261 cacctatcaa ggcattccct acttcaacaa gccgccgctg tttttttggt tgctgaacct
    76321 ctcgacctcg atctggggag atcacgaaat cgcgttgcgc ctgcccggtt cattggctgc
    76381 ggtgggcacg atcctgctga cctatgtgct cgggactcag ttgatgtcgt cccgggccgg
    76441 gttctgggcc gcgctggttg tagccaccag ccacgtcttt ctctggtatg gccgccgcgt
    76501 attattcgat tcgacattga cgtttctggt caccctcgcc cttttcgcct ggattcaggt
    76561 tcagatcctg ggccggagct cacggtggta tctggtgacc ttcctgagta tggcgctggg
    76621 agccatgctc aaggagatgc acggattttt tcttcctctt ctcatcatgg ccctgtatgc
    76681 ggtgattcag cgcgacgccc gcatggtacg tgacagattc ttctggatcg gactggcagg
    76741 ggccattgcg ttgcttgcct ggtattcaca actgttgagc gtcgggtatc agcatcattt
    76801 tctcctgggg agtgcgatca aatccgtgtg gaatagtggc ttgtcgggtg ccgccgggac
    76861 gtccaaagac gggcatcctc tctattggta cctgggcatg atgtgggcgg attttttccc
    76921 ctggtgcgcg ctgctgccgt ccgccctcct gttgttgtgg cagcaacgac cgttgcgcgc
    76981 gcatcagacg gaactggtct tgctggtgtg ggtgctgggc ctcttcacgg cattcagcct
    77041 ggccacgctc aaacgtgagc cctatctgac gaccgtcgtt cccggcatcg gactgatgat
    77101 cgggtatttc tatcatcggt ccagccaatc gtcggatggc tcacgaacca tgtcgccggt
    77161 gttgagactg tttctggtgg tcctggccct ggtgtttatc ggaggcatgt tcttcggcgc
    77221 cgcgccgttg cgccgccgtt ggctggtctc cgctccggtc gtgtctccgc tgttcatcgt
    77281 gattatggtt tccctgggcg gggcgctctt gtatgccgcg atcaccagcc gccttcagct
    77341 cgccttgcgt atggtcggcg tgcttgccgt ggcctatgta gtcctggtgg tgaactatgt
    77401 gtttcctgcg attgatgagg ccgcttcgcc gcgcaaagct accgagtaca tcaaatccgt
    77461 ggcgaaggaa gcaggaccgc ccatgttcct cttcattccc ggttggccca aaaatgaaga
    77521 tgccgtgtat tacttgaagc gggacacagt cgtgcgcgat cttcccgatg aggacgccgt
    77581 acatcacgcc gtgcgaacac aggggcccat ccgggtcatt accgaggaat cgcatatggt
    77641 cactctccag aagcaggccg ggttggtcgt cgaaaccctt caggagttcc tgcaaccagg
    77701 ccgcaagcat ctgtatctgc tgtccatcca acctaaaggc tgaggtcggt tctgctctgc
    77761 gcctctgtcg cattctgcca cagaggcgca gactctctct gtagcagacc tccccgattc
    77821 tgacgtcgtt tcccgatcat tccgtccagt cctgctacgt aggagaagcg cttggctttg
    77881 ctcgtatcag actcagagca gggcgccatc cttgcacttc ttcccttcgg gggcggtctg
    77941 cctatgtgaa accgcaagcc cattaccgca tcgcggtgga gcgagatggt cgtgtgcata
    78001 gctcgtcctg aagttgatgc gggaggcgca ggtcggttct gtcacgaatg gaggaggggc
    78061 atgcatccga ttcatccgat ggtcgttcac tttcctatcg ccctcttgct ggcaagcttg
    78121 ttgttcgatc tggtcgccct gcgatggcgt ggggaagaat ttcgtgcgac gagtttgtct
    78181 ttgctggcgc tcggggtcct cgcggcgttg gtagccttgc tcaccggtca tctcgcggaa
    78241 gaggccgtgg agcatagcgg gacgattccc aagcaggcga tcgaaacgca tgaggaactc
    78301 gcattcgtcg tgttttggct gtttgccggc ctgctgggtg tgaaaatgtt gtcctattgg
    78361 ggctggatgc gagaacgttc tctcctcgtc ctgttcctgg gtgtgggtgg gtcggtgtta
    78421 ttgcttgtcg ccagttacta tggcggcgaa ttggtgtatc gctacggcgc aggggtcttg
    78481 cctcgatgaa gatgatcggc gccctcactc gcatggtcgt agcgatggcc gtgattttcg
    78541 gcgcggcgcc cgtttctgcc gagaagtccg atggccgttc accactgggc gagcgggcgg
    78601 tgttgcgagc gggtcaggtg gtgcagggtg attattttgc gtttggtccg caggtcgaaa
    78661 tctcgggcat cgtgaatggg gatctgtacg cggctggcgg tgaagtgatt gtggatggcg
    78721 tcgtgaacgg cgatgtgatc gtggcgggag ccaaggtcgt gttgtccggc actatctccc
    78781 aggatgcccg cgtggctggc gcgcacgtca ccgtgagcgg gacgattggg agaaacgcca
    78841 cccttgccgg tgccgatgtc catctgacgg agaaggcgac tgtgcgtgag aacgtcattg
    78901 caggcggcgg ctccgtgcag ctgtcgggag ccatcggccg cgatgcacgg gtcggcgcat
    78961 ggcatgcgac cttctccaat gagatcaatc gcgatgtcat tgtggcggct gaatcggtac
    79021 gtctgacatc caaggcctca gtgggcggac ggctgcgata ttggggtgag gtagcgccct
    79081 cgatcgacga ggaagccgtg gtgcatgggc cgatcacacg tcgccccttg ccggaggggt
    79141 ggagcaccga gcgggcccgc caggggctgg tcgggatgcg agtcttcgcg gccatcgcca
    79201 gctttctatc gacgttgctg ctgggactga tcatgctgcg tgtatggccg caattggtcc
    79261 gacaggtcac cggggcgata cgcgaacgac ccgcgccctc tgtgggatgg gggcttgccg
    79321 ccttaatcgt cacgccgctg gttgcgatca gtttcgtggt gacgctggtg gtgttgccgg
    79381 caggggtgat gcttctcgcg ttgtacggcg tggcggcgta tctggcaaga atttttgcca
    79441 tcacctacgc agggcaattg tttgttcgcc gccaggacga ttcctcctca cttgcccggc
    79501 cctttgtcgt cgggctgctc gtctattcac tcctgacact cgttccggtc attggaggtc
    79561 tggtgacttt gttcaccgtg atggctggat tcggcgggtt gctcgtcgcc gtaaagcaac
    79621 gtctcgcagt gagacaggaa caggacctgg tgcaagacac cgcctgacgc agactatgaa
    79681 tcgcccttac acaatggcgc cgcgggggca atcggctgcc gtcaccttcc ttgacggtcg
    79741 aacactgcgt tgtcttggct cctggacgat gtccgatgtc ggaccggccg aacgggccct
    79801 cagggtgatg gattggccgg ctgatggttc gttgcaattt gagggaagcg ggatacacgc
    79861 tctcgatacg ggcggcgcag tggtcctgtc ccgggcagtc agggacgctc agcggcgcgg
    79921 ctgtgacgtc acggtcgaag gactgcgctc tgaacatgcg cagctcatga acctggtgtc
    79981 ggttcattgg gacgccatga tggcgcatgt ggcggcgcga ccgaagggca cggcgcgtct
    80041 ccaatccgtt attagaaatc tcctcgaccg gttcctgcgc ggcttggggt ttatcggcga
    80101 gagtgcgatg gcgctgctgc gggtcggctc gtcgcccact cacctgcgct ggcgcagcgc
    80161 cttgaacagc ctccggctcg atggagtgaa tgcgctcccg atcaccgggt tgctgacatt
    80221 tctcatcggc gtcgtgatcg cgtatcaggg cgccgaacaa ctgcgaaagt tcggcaccaa
    80281 catttttatc gtggacctcg tggggatctc actgctgcgt gaaatcgccc cgctgatcgc
    80341 cgccattttg atcgccggac ggtcgggatc ggcgtacgcg gcacacatcg gcaccatgaa
    80401 agtgacggaa gaactggacg cgttgcgcac attcggattg tcgcccaccg agttgctggt
    80461 gttgccccgg atgctggcgc tgatcgtggc cctgccgttg ttgaccgtct atgccgatgt
    80521 gctgggtgtg ttcggcggga tgttgatcgc cgcgaatcag ctcaacgtca gctttacggc
    80581 ttttctggcg agattcgagg aagcggtggc cttgcggcat ttcctcattg gcgtcggtaa
    80641 ggcgccgttt tttgccgtca tcattgcgct ggtcggatgt tatcagggat tccagattcg
    80701 aggcggtgtg gacgatgtgg gacgacatac cacgatgagc gtggtgcaaa gcatctttct
    80761 ggtcatcatt tttgatgccc tgtgcagcat cattctcaat tggtggaacc tgtagcccgg
    80821 accgctcatg aatacctgct gcggcatccg atacctttgt ccggcagggc gtttctcgtt
    80881 cggcctcctc aacgtatcgc ggatacgcct gcgtcggcct cgcttgcgag acgccctggc
    80941 ggacttcggt ctcggatgcc tcgcgacggc attcatgagt aatccgggct accatgatgt
    81001 ttgaaatccg ttctccggcc cgacccaatt gggagtgcca ggtgtcatgt ctgtagctgt
    81061 gcaaactgag aaccccgtga tcgaggtcag ccatgtctcg acgcgattcg ggccggcgat
    81121 tgtgcatcag gatgtgagcc tcatcgtgag aaaaggcgag gtattcgcca tcgccggcgg
    81181 caatggttgc gggaaatcca cgctcttgcg ggaaatcgtc gggctgctga ctccgacggc
    81241 cggacgtatc agcctgctgg gagctgacag ccgggacctc caagagaccg acggcggttc
    81301 cttgtatcgc cgcttcggcg tgatgttcca acacggtgca ttgttcagtt ccatgacctt
    81361 ggccgaaaac gtggcggtgc ccttacgaga gcatacgtcg ctcagtgccc ggctcatccg
    81421 ggatatcgtg gcattaaaaa tcgccctggt cggtttgccg cctgacagcg gcgtcaaatt
    81481 tccccgcgaa ctcagcgggg gcatgaggcg gcgcgccgca ttggctcggg ccattgtgat
    81541 ggacccggaa ctcttgttcc tcgacgaacc caccgctggc ttggatccga tgatcgccgc
    81601 cggctttgac gatctggtgc ttgatctgaa gcgattgttg gggctcaccg tcgtgatggt
    81661 gacccatgac ctggattcgt tgtggcgcat cgcggatcgc gtggtggtgc tgggggaagg
    81721 gcatgtcctg ggcatgggga ccatggaaca gctggcggcg tcggaggatc cagccgtccg
    81781 gaattatttt catgggccgc gcgggcgcgc cgtacagctg aatcacagcg ggggagagtc
    81841 gcatcgcgat tgagaggtga cgaatggaac cgaaggtcaa ctacgtcgtg gtcggcgcat
    81901 tcgtcctcct gctgggcgcc acggtgctcg gtgcggtgct ctggctgggc aaaaccgatt
    81961 accgcggcat ctacgatcgc tattatgtct acaccaggga atctgtcgcc ggattgagcg
    82021 tcgattcgac cgtcaaatac cgcggtgtgg atgtcgggcg agtgaaggag gtcattctca
    82081 acccggacaa ttccgaggag gtccgcgtga cgctggacat cgtcggaggg acaccggtca
    82141 aaacggatac gcaggcggtg ttggtcacac aaggactcac gggactcgtg acactgaacc
    82201 tgacgggagg aagcaaagac gcgccgccgt tgacgtccgt ggcagggcag gtgtatccgg
    82261 ttatccagag cgttccctct ttgtacggcc gcctggatgg aaccttggcc aaactcttgt
    82321 ccgaccaggg cctgtcgaat ctagtagcca atgtgaacgg gctcgcgcag aatgcgtctc
    82381 tgttcttgga cgaacagaat cgtgcgcagt tccggcagat cctgaaagac ctgggagagg
    82441 tcacgaaggt gctggcggcc aggagcagtc aggtcgaccg cggcgcgcag catgcggcag
    82501 aggcggccga gcggaccgcg cgcctcacgg agcacctggg cgtacaattg ccggcgctct
    82561 tggatcggat gggtaaaagc gcagcggcgc tgcagcacat gacggcagag gtgacacgga
    82621 cgagccgtga agtcggggat atggtcggta ccagcaagcc gggcattgaa caattttcac
    82681 gacagacgtt ggcagatgcc gggctcctgg taagtgaatt gcgacaactc acggcgacgc
    82741 tgactcgagt ggcgcaacaa gttgaacgtc agccgaatgt tttggtgctt ggccgacagg
    82801 gtcagccgaa gggccctggt gaatagagga tgagacgtgc tccgtagtcg cgtaacggga
    82861 tgggtgggat tggtgctggc ggtatacacc gccagcggct gtgtactgcc aaggtcggag
    82921 gactcggctg tccggacgtt tgtgttgatg cgtgaggaga cggatcggca agcggcccct
    82981 gccggggcac cggccggcat gcacggcgtc ttggtcgtcg cggtaccgca agcagcgcca
    83041 ggattcgagc agccgcgcat cgcctatctg cggcggccgt cggaagtgag ctattacgcc
    83101 aatcatgtgt gggccgatag tccctcacgc atggtattgc cgttgttggt tcgatctctg
    83161 gacacgaccg gactctggcg gaccgtggtg ccgatgccca ccggaatcaa aggcgattac
    83221 cagttggatc tgtccggtct ggtcgtgcag caggaatttc tgcagcagcc cagccggagc
    83281 cgggtgcagg tgcgcgcgca cttggtggat gtgaaggcac aacgtgtcct gggtgccagg
    83341 aatttcgaag cacttgaacc ggctccgagc gaggatgcct atggtggggt cttggccgca
    83401 gatcgtgcag tggcaaccgt gctcgccacg atgaatgaat ggatcgcgtc ctgcctgcga
    83461 ggatcaggtc gcgaaacctg ctgagcatga acgaccggtg aggagcgagg atgagcagtc
    83521 gaacatgaca tggtctatgg catgcgcacc gcgctgaagg tcggattcag tttcggactg
    83581 acgtccgggg tcattaccac gctcggcttg atggtcggcc ttcacgccgg gacccattcc
    83641 atgcctgcag tgttgggggg gattctcacc attgcgctgg ccgatgccat gtccgatgcc
    83701 ttgggcatcc atgtggccga ggaatccaag aaccacggga atacgcacga catctgggcg
    83761 tccacggcgg cgacgtttgt ggcgaagttc gtcattgcca ccacctttgc ggtgcctgtg
    83821 ttgctcatgc ctctggacct ggccattgtg gccagcctgc tgtgggggct gaccctcttg
    83881 gcattgctca gttatgccct ggcgcgggcg caaagggtgc cgccctggaa agtcatcggc
    83941 gagcatgtga tcatcggact ctgtgtcgtg gccatgacac attatgttgg tgaatgggta
    84001 cggctgacgt ttgggtgagg atcgaccatg aaggagacgt gtgcagcatg gacataaaga
    84061 cgcaattgcc gaaagtgggc attggccgcc ggtgcggagc gatcgaagca aatctcggcg
    84121 gctaccgcag cctcatgagc gatgacgagt atgaagaatt gaccgcgtta gccaaaagtt
    84181 tgcagggcat caagatttgc cacgtgaact cgacggcggc gggcggaggc gtggcggaac
    84241 tgttggcgcg gtatatcccc atgctgcagg cgcttggcgt acaggctgac tggcgtttga
    84301 ttcatggtga gccggagttt tttgccatca ccaaggcgtt tcataacgcc ctccagggcg
    84361 ggcattacgg gctgggaacc accgagcagg acgcctatct gaaggtcaat gaacgcagtg
    84421 cgcaggcgct gggcaatgac tacgatctgt acatcgtgca tgatccccag cctgcggcca
    84481 tacgtcactt tgcgggatcg cgggatgcca aatggatctg gcgctgccac atcgacagtt
    84541 cggcgccgga caaggaggtc agccggtttc tgcgcccctt gttggaagag tatgaagcgg
    84601 tagtgttcac catgagccaa tttgtcctgc cgcagttggc ggcgagacga gtggcgttca
    84661 tcgccccggc aatcgatccg ctggccacga agaacatgga gttgccgatt gatctctgcc
    84721 gccgcgtcat cgccgactcc ggcgtggatc ccgccaggcc gttgctgctg caggtgtcac
    84781 ggttcgatcc ctggaaggat ccgctcggag tcgtacgcgc atatcgcctg gtcaaagaag
    84841 agatcccgga ggtgcagtta gtgctgatcg gcgcgatggc gggcgacgat cccgaaggct
    84901 gggaaattct ggatcgcgtg gaggaagagg cggcgaacga ccccgacctc tttgtcttta
    84961 cgaatctggc cggtgtggga agcatggaag tcaatgcgtt ccaacatggc tgcgatgtcg
    85021 tgattcagaa atcgttgcgg gaagggttcg gactggtggt gtccgaagcg ctgtggaaag
    85081 aaaagccggt cgtcgccgga aataccggcg ggatccccat gcaattccct gagccttttc
    85141 aacggaacct tgtcgacagt gtcgaggcgt gcgccgagcg ggtgcgccat ctcctgcatc
    85201 atcctggtgc gcgtgcggag ttcgggcggg cgggacgtga acatgtgcgc cggcattttc
    85261 tgatgccgcg gctggtgcgt gatgaactac ggctgatcaa gcaagtgctc aacgcatcat
    85321 gatgcagcaa aacgataaag ctcgcgtggc ctacttttcg atggagatcg gcctcaatcc
    85381 tctgatgccg acctatgccg gagggctcgg ggtgttggcg ggcgatacga tccgatccgc
    85441 tgccgacctt ggggtaccca tggtcggagt cacgttgctg caccgtcacg gctattttta
    85501 tcaacgcctc gaccggacgg gacgacagtc ggaggagccg gtggcctggg cggtgactga
    85561 ttttgccgag ccgctggacg cccatgtctc cgtggagatc gacggtcgca acgtgcaggt
    85621 ccgcgcatgg cgccatctgg taacggggat gtcaggccat accgttcctg tctaccttct
    85681 cgatacggat gtgccggaaa acagctcatg ggaccggaca attaccgatg cactgtatgg
    85741 gggcgacgcc tattaccgcc tgtgccagga aatggtgctc gggttcggcg gatttgacct
    85801 gttgcgcgca ctcggacagg gggacatctt tcgttatcat ctcaacgaag gacatgcggc
    85861 tttgctggtc cttgccctga tggaatccgc tatcgggacc gcaccggtga acggcgaggt
    85921 gccgcccgaa gtgatcgaga aggtgcggga gcaatgtgtc tttacgacgc acactccggt
    85981 accggcgggg catgatcagt tcccggccga tctggcgcat cgggtgctcg gacgccggcg
    86041 ctgcgagctg gcgcaggcgt gccagcatca aggcgcgttg aacatgaccc tgctcgcgtt
    86101 gcgagggtcc cgctttgtca acggcgtggc gatgcggcat ggagaggttt cccattccct
    86161 gtatcccggt tatccgatcc attcgattac gaacggcgtc catgccgtgg cctgggccgc
    86221 gccttcgttt cagaatctgt acgatcgcca tctgccggac tggcgatacg atcatctgtc
    86281 gctccggtac gccattagca ttccctcatc ggaaatctgg gctgcccatc gggaagccaa
    86341 gctggcgctg gtggatgaag tgaatcgcga aaccaatgcc ggattcgatc gcgatgtact
    86401 gaccattgga ttcgcccgcc gcgccacggc gtacaaacga gcgacactgg tttttcagga
    86461 tatcgcgcgg ctgcagcgcc tgatgaaggc gaaggggcag atccaatttg tcttcgcggg
    86521 caaggcgcat cctcaggatg aggccgggaa agacacgatc caacggatac acggaatgcg
    86581 tggacaattg cccgttgcgt atctggccaa ttacgatatg accctggcgt gtctgctctg
    86641 tgccggtgtg gatgtgtggc tcaatacgcc gctgccgccg atggaagcct cggggacgag
    86701 cggcatgaag gccgcgatga acggcgttcc cagtctcagc gtgctcgacg gctggtgggt
    86761 ggaggggcat atcgaaggcg tgacgggttg gtccatcggt gacaaggttg aagcctgtct
    86821 cgctccgggg tcggacatgg aggcctgtca tgccgccgcc ttgtatgaca agctggagca
    86881 gcaagttctg ccctgctttt accaggaccg cgagcgattc atcgacatca tgaaacatac
    86941 gatcgcgatc aacggcgcgt ttttcaatac gcagcgcatg gtgcagcaat acttgcggaa
    87001 tgcgtatcga ctggtcggat agaccggcca ccggagcata ggcgtatggc tcagcctcac
    87061 gacagcatgc agtccgatta tagccacttg ttgacgctct ggactgccgg ggtgcgcgat
    87121 taccacacga tgctgtccga ctatctgacc gccaactcca tctttgtcgc cgtcatcggc
    87181 ctgctcgtgt cacgggaatc cctggcggtg ccgtttaccc tcgtgattgt cctactgagc
    87241 gcaatcggca ttctcatgag cgtacaaatg gcgatcgtgt tgggccggtt ttccgggcag
    87301 aacgccttgt gggaatggca gctgcgcggg attgaatcca tgccggattg gagggaacgg
    87361 aagccggtca gtatgctgta ccggctccgc gagcatcgcg aaacgattgt ggatgagacg
    87421 aatgagccgc gctccttcga gccgtcctgg gcattccggc agcatcgcca atggtgggcg
    87481 catcgggcga tcagcttccc atggtttttt ggtacggtct acggcctctt tttgttgtgg
    87541 gcggtgacgc agatggcgag gtcgagcatg
//
LOCUS       DAOQDS010000005        84017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_83_length_84017_cov_19.767228, whole genome shotgun sequence.
ACCESSION   DAOQDS010000005 DAOQDS010000000
VERSION     DAOQDS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 84017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 84017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84017
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83_length_84017_cov_19.767228"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..130)
                     /locus_tag="PK589_01915"
     tRNA            complement(55..130)
                     /locus_tag="PK589_01915"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(95..97),aa:Thr,seq:tgt)
     gene            424..1839
                     /locus_tag="PK589_01920"
     CDS             424..1839
                     /locus_tag="PK589_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ18378.1"
                     /translation="MSQSHILVIDDDPAVRQLLAETLTSEGHQVTVMSSGLDGVEAVK
                     DQPVHVVLTDLQMPGIDGLETIDRISKIDSKVIAIVMTGYGTVDYAVRAMKAGAFDFI
                     TKPFEPDTVAVVVRKALDVYKLKQENHLLRKAVRDQYRLEHLVGTSAPMRMVLDFVEK
                     VADSDSTVLIEGESGTGKELIARMLHFNSMRRDRPLVPVNCGAIPETLLESELFGHEK
                     GAFTGAAHTRLGRFELAHGGTIFLDEVGEMSLPLQVKLLRVLQERCFERVGGTRTINV
                     DVRIIAATNQDLAQAVQERRFRQDLYYRLHVIPIHIPPLRERRSDIPLLVNHFIAQFN
                     QLRRTEILGMEPDALVRMTEEEWPGNIRELENMIERLCVLKKRGMITLADLPERALKT
                     AGGKAVEAPEQFIRFSEDGINLTKELEHYENRLIGEALRKANGITSRAAQLLQVNRTT
                     LVEKLKRKGFDPKSHGYSIQN"
     gene            2116..4173
                     /locus_tag="PK589_01925"
     CDS             2116..4173
                     /locus_tag="PK589_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ18379.1"
                     /translation="MRQELPAMMAEPSELVRGALPDPGPRFERLPEIPEWKQFEQGVG
                     LYHQGQYLEARLHFTNMLRDRRESPLRSSIQAFLAESTLQSHDPGVRPLDIIDQYKTL
                     MRENPQSTNAKRAAWRIGDVYRIEGWFQEAQIAYQHALSLSEIDSFDANRAMLGLAYV
                     QRGFKNWKESTQTFDHVLKRTVDPTLLVSASLGEAHSLYRLGRMKDADALYESISGRW
                     PAALRSDAFALLRYADTAGEAKRGPVMREQLLHFYNLYPNRPETPFVLTYLADSYKEA
                     GRWAESSIFYAALLSQYPEAPVAATARLRYADVQEHLDPEGEEINLRHTISAHLDHVP
                     LKAGEMLSPRRLFEQSAKDNEGSIVGSEALFHLGETLERAGKPEGAVRAYEQVVLRSG
                     KFDNDPWPEKGGVQLVRFLRPRLEAALKAEDDFELINIFHRHGPFADRLYAGTELLLK
                     IAEAHRRLGFPVESARLFQSLVRDAKAEKFHEAALMGLAYSYMEQKDMRAARSVFERY
                     RLEFPTGRFSGEALRGILNSFEGEGNLAALLKLGKQWLAHNPRHHDRTMVQLKLAAAL
                     AQSKQPAEAAAIYDGLIKAGQELPAADLLRHAEVLARLNRRSQALAMYKEAVVAGLEL
                     EQEAWAQLQMVQLARGEKREDFAKNGLRALSVNPDSLVRRMAAMLDNELPEPPVDKRG
                     KKP"
     gene            4170..5459
                     /locus_tag="PK589_01930"
     CDS             4170..5459
                     /locus_tag="PK589_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ18380.1"
                     /translation="MIRQEGDTTNNDLLTRAFRDFDQAATVLQQSYDALTTRLQQMDL
                     ELAQTNASLREHLRETEEMRAHVTAVLESLDTGVIVADSQDVVVRCNHSTEQLLGVPQ
                     AQLRGRQASALLAEIRKDHGEYPLVLPSGVTIALSQTDLTDESGNPIGKLVLIHDVTR
                     IRQLEDRLQRRNRLEAMGQMVGSIAHEIRNPLGSVELFASMLRKDLRDQPQLRSYAEH
                     ISMAVQSMDQLLSNLLVYTRPDCSRLGWHDTEVLIREVLTLASHAIPPDTVDVRCQMH
                     PLVPQLWCDGPKMKQVLLNLVLNAVQAMPKGGTLTLTACPSPPEIQEVPAVHITVSDT
                     GTGIPAELQSRVFDPFFTTKDQGTGLGLAIVHALVEAHHGRIDVESRPGHGTSFVITL
                     PRGPRQPSSTPVSLVTVSAPSERSRHITSITEEKRYE"
     gene            5456..6874
                     /locus_tag="PK589_01935"
     CDS             5456..6874
                     /locus_tag="PK589_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ18381.1"
                     /translation="MSAQPALQGPSASADEARMVLIVDDEPSMRTALSETVRRMGYQV
                     RSAIDGADAIEQVERLKPWLVVTDLKMPRLNGLELVKALKQKAPQTFIILMTAYGTVE
                     TAVEAMKYGANDYILKPFSTDLLERVILNLQASAEPDEQDAPAALEARAILTQDPGMI
                     RLLTTLEGVAASQATVLISGESGTGKELLARYIHARSPRAHRPFVALNCAALPDSLLE
                     SELFGHERGAFTGAIQKKLGKFEMAHTGTLFLDEISEMNLGLQSKLLRVLQEREVDRI
                     GGRDPVPVNIRVIATTNRSLYHEVTQGRFREDLFYRLNVFPVTVPPLRERPGDIPLLA
                     RHFLRSSAQRNGLTVPTLSERAIADLQQRAWKGNVRELENVMERAILVTTGNAVDVDH
                     LMPGDGTVPIQPVETVETLVAPSAHGSLWEMERDLIFKTLARVKENRTHAAKELGISI
                     RTLRNKLREYRDLGYQVEAEKP"
     gene            7045..7452
                     /gene="flgB"
                     /locus_tag="PK589_01940"
     CDS             7045..7452
                     /gene="flgB"
                     /locus_tag="PK589_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNJ18382.1"
                     /translation="MTIFDRTMQLLERSLDLRGARQQVIAANIANEETPKYRATDLNF
                     GQALANAQQGKLPIALVSTHQQHIGPKGSGFQRVQGRIEEVPAGDLPLDANSVNIELE
                     MAKMSDNAQQYNTSASIISMRFKTLLSAIREGR"
     gene            7514..7963
                     /gene="flgC"
                     /locus_tag="PK589_01945"
     CDS             7514..7963
                     /gene="flgC"
                     /locus_tag="PK589_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNJ18383.1"
                     /translation="MDLSDSLAVSVSALDAQRHRLNVIASNLANAQSTKTSTGGPYRR
                     RDVVFQAAPVSSAFQKAFTQVSKGSGRHALDGVKVARVIEDQKPGQTVYDPRHPDADK
                     KGFVTMPNVNVMEEMVNMIGASRAYEANVQAVNATRTMWNRALEIGR"
     gene            7966..8277
                     /gene="fliE"
                     /locus_tag="PK589_01950"
     CDS             7966..8277
                     /gene="fliE"
                     /locus_tag="PK589_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNJ18384.1"
                     /translation="MSDLHIAGANLIKPIEAPGLQQAGQTGEAGGANFLGSLKEAIGH
                     INDAQAGASQAVESLVTGQNTNIHQTMVALQQADASFQLMMQVRNKLVTAYEEIQRMQ
                     I"
     gene            8325..9884
                     /gene="fliF"
                     /locus_tag="PK589_01955"
     CDS             8325..9884
                     /gene="fliF"
                     /locus_tag="PK589_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNJ18385.1"
                     /translation="MFSKFSQFTINQRFIILLALAGSIAGLVAVTLWTQQPDMQVLFA
                     NLATDDAAGIIDKLKDAKVPYETTNGGTTILVPNAQVHDLRLEMAGQGLPHGGGVGYE
                     IFDRTTMGMSDFVQKLNYRRALQGELARTITQMPEVERARVHLAIPERRLFATEQDRA
                     RASVVVSLRSNQNLSKAQIQGIVHLVSSSVEGLQARDVTVVDGHGNLLSNSSSDESAG
                     LSGTQMEYQRTLEKNIETRIQTMLERIVGVNKAVVRVSSVLDFRKIETTEERYDPNGQ
                     VVRSEQRGQEKSSGVNGTTGGVPGVESNVPGGAEGEGGQTSSNNNQTKNETVNYEISR
                     TVSRIVEPTGTIKKLSVAVLVDGTYEGGGKAAEGAAEQPKKYVPRSEEEMKRIEEIVK
                     KAMGYSTERQDQVEVVSIQFGLGVEEPVGAGVEAAADSTRAWMPYIRYAVGGLLFFLI
                     LFMVVRPLMVMLVQSAPVEAVGETPALPASVGQVEAAISGKQPGQILDMAKNNPANTA
                     VVVKQWLKSNA"
     gene            9890..10891
                     /gene="fliG"
                     /locus_tag="PK589_01960"
     CDS             9890..10891
                     /gene="fliG"
                     /locus_tag="PK589_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNJ18386.1"
                     /translation="MAINKELSGAQKAAILLRAIGEEAAAAVMKSLDPKDIRKLGEFM
                     KETANITKQEEDSVIAEFEQASSSGEVQFEGREFMEAILKKALGPEKAARMMESLNTK
                     TYPGIDALKWVDARTVAQILKIEHPQTIAVCLGQMEAEQASAVLALLPVHLHADVSLR
                     LATMQEVQPEVLAELSDSLQEILSASMGMSSMSVGGAELMADILTRVDKNTEGAIMAK
                     ITERDQALAESIRALMFVFDDLVDLDSRGMQELMKEISKEDLPIALRGATPEIKDKFL
                     KNMSSRAAEMLKEDMETRGPVKVSDVEKSQQNILKVCRKLEEEGRIVIGGGGGEELL"
     gene            10892..11941
                     /locus_tag="PK589_01965"
     CDS             10892..11941
                     /locus_tag="PK589_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ18387.1"
                     /translation="MGSAAAGTGVSMAASPESEKELSPRAVLIVDDEPSICLLLAEML
                     ESTGHPVLTAGSVPEALSHLRTRSVAVVIVDVQLGGADGIAFLQQALDIDNRLLGIVM
                     TGHGNIELAVRAMKSGAADFLTKPFQVELVRLAVSRLLELYRLRQENTVLTRTLIRSG
                     NIQLRPVPLADFSRGNRPFGTEDLTEFERGVAEGEKRSTERVAAARQREQSLVASLMT
                     RLEETWQSLHETVEEEIASLSFMIAHKVVRDVVTEKRDLIVTQVRTALAHLHESGLVR
                     VRVHPSDLTVLESARTALSQTPHGLLTLKFETDPSISPGGCLVQAQSLLIDATLDTQL
                     LRLGEALRKRDAGEA"
     gene            11925..13238
                     /locus_tag="PK589_01970"
     CDS             11925..13238
                     /locus_tag="PK589_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNJ18388.1"
                     /translation="MPVKLSARLEHVEPLSVTGRVAQAVGIVIEASGPFTSVGEVCEI
                     TREGGHGSVMAEVVGFREDRVLLMPLGEMQGIGPGSRVVMKGHVASVPVGPQMLGRIF
                     DGLGHPLDGLPALRSDVRVPLYAPPLNPLHRARITQPVDLGIRAINALLTCGQGQKIG
                     VFAGSGVGKSVLLGMISRYTQADVRVIALVGERGREVKEFLERDLTPEARARSIVVVA
                     TSDQPPLVRIRGALVATAIAEYFRDCGKHVLLMMDSLSRLAYSQREVGLAIGEPPTTK
                     GYTPSVFAVLPKLLERVGPAQSGGSITGLYTVLVDGDDLNDPVADAVRSILDGHIVLS
                     RELAARNHFPAIDILQSTSRVMRDIVTPAHYAAARLVLERTALYRRSEDLITLGAYKP
                     GTSKDLDKAVSAHDGITAFLRQETKQAVGLPQSIDALLALAGTIQ"
     gene            13235..13675
                     /locus_tag="PK589_01975"
     CDS             13235..13675
                     /locus_tag="PK589_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HNJ18389.1"
                     /translation="MNVTVLRAYAVQLEEVAKLELAELSHALQQTVNRMAMLESRAKE
                     DADRYLHQVRAGSTVDQVYGHLDAMDQAIAARKGLEQALATEQARMEQKRAELLEAMQ
                     YRKKLDLLDARAALEIRRRRERQDQQLLDERAWRRSPLHKGERA"
     gene            13672..14304
                     /locus_tag="PK589_01980"
     CDS             13672..14304
                     /locus_tag="PK589_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18390.1"
                     /translation="MMETGSRSEQGRCVGVVALIAFGAVFLTLITTAHATEEVKPAAV
                     PQKEETKPESVKKDVQGPATSAPREIYEMLEQRKRLLDKRETAVRGSETHLLELKAEL
                     EQIVTRHEQAVEAEKKRRQAAQAKGAGEAEKAKTTAKATPAVNVNQAQLAKIYEAMPA
                     EEAAARLERMPERKALEVLRLLKGKSAGAILAEVKPERAARLTEQLLTTP"
     gene            14501..16096
                     /locus_tag="PK589_01985"
     CDS             14501..16096
                     /locus_tag="PK589_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNJ18391.1"
                     /translation="MATDMQPVFNGFPSRSSQGIQDARDRQPAVHGSDPSRNFESVLG
                     RAEARQTPPKHEAPTPRSGEKAGLHARQVSRSISDSSSQPSERSSVAPSDDHSSNRCE
                     CAGEKAQPSTDKSEEKSTTKESKKKDPGQDAVSQDMLLASMMVPTAQAAPPEQQTPQA
                     EAQSDSHSETSVNAAAAISSDRAEDGSASVTTLVAATSEAGAGTQEVLAGQSDPKADA
                     GKDAAQAKERAENGNEMVQTTAKELPSLAGDNSSPDARVEDRTDSTAAARTDADRMNP
                     AIAAMTSSKQNPHMAEQGPSEGADRSAILDALAQGASLSEGQAGSSTGRFLEPDGQNL
                     SDSSGGTDRSLPNQVPSVEQNARPAFMDRINDVHQSTPSVNDSSSGRSESGQTTAVLR
                     ASESERVGELRGMAPVSQSVTLDLDPLDMGPLRVRVMMSDQTVHAHIRTEHGELGQGL
                     LQQGQSLESSLRTSGLEMGMLRVTVDQQQGRGDNAWMFQQQQQQQNRPSSPGLSQTVA
                     REDERGARGERGAGSNERVSFFA"
     gene            16108..16398
                     /locus_tag="PK589_01990"
     CDS             16108..16398
                     /locus_tag="PK589_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HNJ18392.1"
                     /translation="MDISKVITQTPAADPSTPTTEGPKPLGKDDFLKLLVTQLQHQDP
                     LKPMENEAFIAQTAQFSQLDQMTKMVSLMEKSVALQESAQNKTTQTGTTAVA"
     gene            16472..17695
                     /locus_tag="PK589_01995"
     CDS             16472..17695
                     /locus_tag="PK589_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNJ18393.1"
                     /translation="MGILTSMFTAVTGLSSYGNAMGVIGNNIANVGTAGFKSSRATFS
                     DLISSSMAGGAGSDQIGLGVYLNDVQTNFSQGSMTTTGNTFDLAIDGNGFYLVRNASG
                     SNFYTRAGQFKVDSVGQVVDASGALLQGYQADTLGNITSTIGGITLTSSAVPPQSTST
                     ATILGNLNANATAPTAAFATTDTTSYNFSTGMTFYDSLGNSHQMQLYFRKTAANAWGV
                     YSQIDGGAATAQTNMTFNASGAVTAGGTQTITAALTNGATTPQSISVNLASLTQFGAA
                     SGVQSQTQNGYSSGTLDRISVDKEGKVMAQFTNGQTRALAQVVLSRFTNPDGLANVGD
                     NHFLETVESGAALAGAPTINGLGRVLSGTVEQSNVDLGKEFVDMIITQRAFQANSRAI
                     TTSDEMLQELVNLKR"
     gene            18028..18567
                     /locus_tag="PK589_02000"
     CDS             18028..18567
                     /locus_tag="PK589_02000"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNJ18394.1"
                     /translation="MSEAAEEKAPAAPAGIPMKMVIIIVGGVLILALGGAFAMMKMMG
                     GSSAEKTQAEGGGEKAAAHGAAEEKSGGEHGAVADASAIADLDPFIVNLADSPEIRYL
                     KVTMKVEADNAGTVEQIKARTPQIRDAILVLLTSLDSATARSPQGKHKLRDDITERIN
                     GLLPKKSVKSTYFTEFVIQ"
     gene            18589..19560
                     /gene="fliM"
                     /locus_tag="PK589_02005"
     CDS             18589..19560
                     /gene="fliM"
                     /locus_tag="PK589_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNJ18395.1"
                     /translation="MDKILSQEEIDALMGGVMSGDVETAPKEEEEPGGVKTYSLTSQE
                     RIIRGRMPTMEMINDRFTRQQSVSWTSVLREKIEFSVLGTQIIKFGDFIQKVPVPSSF
                     NLFAMEPLRGNGLYIMDAMLVYLIVDFFFGGKVQTHVKPEGREFTTIQLRLIKKLVQQ
                     ALVDLEKAWQAVMPVKLNYLRSESNPQFAMVVTTSEIVVVVTLQVHLGDITREMFIAY
                     PYSMLEPIKEKLYSGLFADNVEQDSSWGSRFKDSLQECDVAVTVQLGTAKINVEDLMN
                     FSPGDVLMLDQHPGDPMLCLVEGDPKFHGQPGVFKGNHACRVTKVLS"
     gene            19590..19958
                     /gene="fliN"
                     /locus_tag="PK589_02010"
     CDS             19590..19958
                     /gene="fliN"
                     /locus_tag="PK589_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNJ18396.1"
                     /translation="MGNGDTTPQADASSGADMSPQATSFPPVDNQGAVPANKNIDFVL
                     DIPLQVTVQIGSTRMLIRELLQLGQGSVIELNKLAGEPMEVLVNNKLVARGEVVVVNE
                     KFGIRLTDVVSPNQRIQQLG"
     gene            20015..20383
                     /locus_tag="PK589_02015"
     CDS             20015..20383
                     /locus_tag="PK589_02015"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNJ18397.1"
                     /translation="MEIWESVVRMVSALGIVLALILGLLAIVKSRVGQRFLPVGGAPL
                     IKVLGSSSLGARKQVMLVAVAGEVLILGTTPTDLVSLGKVTDPEQVKRLLLEASSNMA
                     LSPAQAVGSEYREETLHAGR"
     gene            20370..21170
                     /gene="fliP"
                     /locus_tag="PK589_02020"
     CDS             20370..21170
                     /gene="fliP"
                     /locus_tag="PK589_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960008.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNJ18398.1"
                     /translation="MPDDNLTSLQQAKKGGAGTVPRCMGWVGLVLLVVLAGASSVHAE
                     GPSLTLDLGQSGTKQTAVVLQILALLTVLSLAPALFIMVTSFTRMVIVLSFLRQALGT
                     QQVPPNQVLISLALFLTMFVMAPVGQAVYKDALQPLLAEQIGYEDAWNRGIQPIRGFM
                     LKQMRDKDLELFLELSHSPKPAQVDQVPTHVIIPAFVLSELRISFQIGFLLYIPFLIV
                     DMVVASVLMSMGMMLLPPVMISLPFKLILFVLADGWYLVVGSMVKSFQ"
     gene            21182..21451
                     /gene="fliQ"
                     /locus_tag="PK589_02025"
     CDS             21182..21451
                     /gene="fliQ"
                     /locus_tag="PK589_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNJ18399.1"
                     /translation="MTIETVTEIGRQAIETAMLVSAPVLGLSLVVGLMVSTFQAMTQI
                     NEATLTFVPKVLAVFAATLLFLPWMMGVLIAFMTHIITSIPTLIH"
     gene            21467..22255
                     /gene="fliR"
                     /locus_tag="PK589_02030"
     CDS             21467..22255
                     /gene="fliR"
                     /locus_tag="PK589_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNJ18400.1"
                     /translation="MNVAQTLHLALPQFQSFSILLVRIAGIISVFPILNTLTIPMPVK
                     AGLVTMLGLVLAPIIQLPSFPNDPALVVAGIGSEFLIGLTIGLAVRLLFSGFQVAGEL
                     VGTQMGFSAIQMFDPMSHQNAPIIAQFQLTLGSLVFLSLNAHLMVVHAIGMSYEFVPP
                     FGAKLSDSIATDIIHLAQNMLGVALKMAAPVFATLLIVNTVLAILGRAVPQMNVFVLS
                     FPITIGAGLFAMSLAMPFTLSLFEKEFENLVETILSLLKGLGHG"
     gene            22248..23330
                     /gene="flhB"
                     /locus_tag="PK589_02035"
     CDS             22248..23330
                     /gene="flhB"
                     /locus_tag="PK589_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNJ18401.1"
                     /translation="MADSAQNRTEQATPKRKAEARSKGQIALSRDAAMAVALLGSFAA
                     LYWMTPGIMERLRHSLQRWLSQSMEDSTLRALSLDHLHLILRQIGIDVFVMLGPVVAG
                     IAVVGVGANLMQTGFLWRKDGLSIDWSRISPVGGFSRLFSVRALSELVKSWLKILAIG
                     SVGYLTIKEDMLQFLPLTQFGMETLLPTVGWATFKAALMMSGAALVIGALDYGYQRFE
                     WERGLRMSRDEIKEESRAAEGDPGLKAKIRSTQREMSRKRMMAAVPTADVIITNPTHL
                     AVALKYDSKAMGAPIVVAKGAGFIAEKIREIGRQHGVMIVENKLVARTLYKLVEVGRE
                     VPEDLYRAVAEILAFVYRVRGKLPPA"
     gene            23351..25459
                     /gene="flhA"
                     /locus_tag="PK589_02040"
     CDS             23351..25459
                     /gene="flhA"
                     /locus_tag="PK589_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNJ18402.1"
                     /translation="MAKDNTSIPSTSLVKHPDILMSVGVVGILMVMLVPLPRFFLDLL
                     LSFNITLSIIILLVGMQVRRPLEFSVFPSILLMVTLLRLALNIASTRLILLHGNEGAA
                     AAGEVIRAFGNFVVGGNYTVGLVVFTILVIINFVVITKGAGRVAEVAARFTLDAMPGK
                     QMAIDADLNAGLINDKEARQRRKEIAQEADFYGAMDGSSKFVRGDAVAAVVITLVNIL
                     GGLTIGVLQQGMTVAAAAQTYTLLTVGEGLVAQVPALIVSTAAGIVITRAASEVNLGF
                     EITRQVLISPKAIGTASGILLAMGLVPGLPHLAFLALGSLTGWMAFQLNEHQKTAVAT
                     PEPESPQVKAEEAATQVVPLDLMEVQVGYGLIGLVDGGQGGALLDRIKGLRRQFAEQM
                     GFVLPPIHIRDNLQLRPNEYAALLKGVELAKSEVMPAHVLAIDPGTAQRGIIHGLPTK
                     EPAFGLPALWVPEQQREQAQMAGYTVVDPGSAIATHLSELIKRHAHELLGRQEVQGLL
                     DQLGKNHPKLVEEVVPNLIPLGTLVRVLSHLLREGVPIRDLRTILETLADHAASTKDA
                     DILTEVARQALGRTITKQYLAPDGSLPIIGLDPRLDRTLADQANAVGQGNQWIPDPMV
                     AQKLLSALKQAAERMVGRGHQPVILCSPSLRRHLRKLTDRILHSVPILGLNEVDSVTR
                     LQAMETVRLDLDGGDTRSLA"
     gene            25456..26739
                     /gene="flhF"
                     /locus_tag="PK589_02045"
     CDS             25456..26739
                     /gene="flhF"
                     /locus_tag="PK589_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNJ18403.1"
                     /translation="MKVRTFHVASMHEAIRSIKDTLGPDAVILSTKRVRSWDNGFGLL
                     GGSMLEVMAAIEDDAAVPESAPILGEDERSAPAVAQAPAVPAEESRFQKTLQGAIEKR
                     EDQPRPASRQASFVPQELSRTAKSSPVTKDGQGTTFQSFQRVYEDLLAQGVEHATAEA
                     CLRELRETLVEQGTSQRNSSLQLLRTVLLDRVSTAGPLLSNAGEQKIAMFVGPSGVGK
                     TSTIAKLATHYGIVEQRSVALITMDTYRPAAVEHLRLYAEVLGIELAVASSCEDARAA
                     IAQAREAELILIDTAGFNPYEPIAAQRWRGLLNGALPMEVHLVLAAGTRVPDIVVSTS
                     QCVDVPSLRLLFTKLDETTGYGGIFEATHRTGIPLSYWGTGQRVPDDLVQAQVNRLGD
                     LLLGGQVRTPGEQNGQPWIRQAAPAVVPGIKTYTR"
     gene            26767..27633
                     /locus_tag="PK589_02050"
     CDS             26767..27633
                     /locus_tag="PK589_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNJ18404.1"
                     /translation="MAIEPDILDPQLNTFDGSVGPSRTQVITVTSGKGGVGKTNVVAN
                     TAIALAQTGKRVLVLDADLGLGNVDILLGLTPKYTLEHVLAKTCRLEEIALTGPNGIV
                     VLPASTGISQLTVLTEAQQLILQEELERLASTMDVLLIDTGAGISSTVTYFAAAAQSI
                     VVVVSPEPTSLTDAYALMKVLLRQYRERRFHVLVNMVKSPRDAARIYRKLEVAVSRFL
                     HISLDYLGAIPQDDYVPMAVSQQRAVIDLFPHAPASRAFRELTEAVAQLTPPALPKGT
                     VQFLWQQLLRTH"
     gene            27653..28414
                     /locus_tag="PK589_02055"
     CDS             27653..28414
                     /locus_tag="PK589_02055"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HNJ18405.1"
                     /translation="MMEARKMSATGNASRRVIAEADRERVIQEFTHVVKAMAHRLAFR
                     LPAYMDAEDLMSVGIMGLMDAMDKYDPTREAKFKTYAEFRIRGAMLDEIRSMDWIPRS
                     VHERISLLQKTYSELLHRFGRPPTDQEVGKELKMSADELDEFLTRSRGAVVISLDDLG
                     VQDADGHKIISMLTDSNQPDPLSVLVNERARHVLEAAIQDLPEKERLVLSLYYFEELT
                     MKEIGQALKVTESRVCQIHSKAILHLKARLEPVDV"
     gene            28581..29444
                     /locus_tag="PK589_02060"
     CDS             28581..29444
                     /locus_tag="PK589_02060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNJ18406.1"
                     /translation="MPPSVTGESSAAVEPEPDHHHAWSDNDALTRSYDAARFGVLACL
                     LFMAGGLYWTAAIGLLTISALTAYPVMVSIGVAFTLMIFGATFWTPRSRSSAVRPLWS
                     AAESGSKTTYDPVTGLPLNRLFLSLLNQALIRGQKHGRQVAVLIIELDYFTPAAESPA
                     ELNRHLMYRIEAARVKSALRTTDTVARIAERTFAVLVDQITDADNLLLLARKMQGAIA
                     LPITLDHHEVFLTGRIGLALSKRDDLDPNALLEAAARALEMARTDASGMSGIETALPQ
                     PQTDSTSTIAA"
     gene            29723..30490
                     /gene="flgF"
                     /locus_tag="PK589_02065"
     CDS             29723..30490
                     /gene="flgF"
                     /locus_tag="PK589_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HNJ18407.1"
                     /translation="MNRAIYPILSGAVAQEKQLQVFANNLANVNTAGFKQDQQGFRGL
                     LARGGSVGMGAASSGITSTISTRPAGPTERVFTEANGMRTTFEPGRIRITGNPLDLAI
                     QTDGFFEVKTPDGVRYTRNGIFSLDGQRRLVTNLGHPVMGTKGEIKVPPGNIQINAEG
                     GIQVDGNAIGTIKVVEFPPNEMPQKFAEGLFTGGKPSVSKKPQLQSGHIEESNVNSLS
                     EMVKMIQGMRSYESAQKLIQTLDHMTDTAIQDLGRVQ"
     gene            30501..31292
                     /gene="flgG"
                     /locus_tag="PK589_02070"
     CDS             30501..31292
                     /gene="flgG"
                     /locus_tag="PK589_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNJ18408.1"
                     /translation="MIRAMWTAATGMTAQQLNVDTIANNLANVNTNAFKRSRAEFGDL
                     LYQIQRLPGTNASNVGVFPVGVQVGGGVRPITVAKEWMQGNMRQTSNDLDLAIDGAGF
                     FQVARPDGTIMYTRNGSFKRDNVGNLVTGDGDQLTPVITIPSGALKIDIGQDGTVSVL
                     LPGVTQASQVGQIQLVRFDNPSGLVAMGGNLFLDSFASGPAQQGTGGFSTGFGTLQQG
                     FLESSNVNLAEEMVNMIIAQRSYEINSKTIQASDEMMQIANNLRR"
     gene            31350..32123
                     /gene="flgA"
                     /locus_tag="PK589_02075"
     CDS             31350..32123
                     /gene="flgA"
                     /locus_tag="PK589_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNJ18409.1"
                     /translation="MATISLLASAVLPAMAGERAPIRPLFRVHEALVQGAHSLPLARG
                     KHTIYPEQIRGVIRDFVKRELAGRAVDCQVALGDPQQPIVVPSGTMDLQVSANHSEEP
                     LGRRVFQIHLTVNGRFIKTVEATADVAAIVEVVVPVRSIKVDEEIAADDVATERLVLY
                     DLKQPFAMNPSDVVGKAAIRPLPPQNAIRLTSLRRPFAVHKGDRVTIEARQGGLSIQT
                     VGVTKSHGELGQTITVSNVDSGKDLRATVVGPGVVRVSF"
     gene            32242..32826
                     /locus_tag="PK589_02080"
     CDS             32242..32826
                     /locus_tag="PK589_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNJ18410.1"
                     /translation="MPPPKTTGSLWQEENGRAYLYEDLRAMRAGDIITILISEKHKGS
                     KSADTSAEKDSTVSNGLGGTGMGYLGLPGIRLGGEAKRGFGIDASAKNKFGGKGATNR
                     EDTLTGTISAIVTEVLPNGDLRIEGRREVTVNSERQIMTIAGIVRRVDVNTKNTVQSS
                     AIADAKIEYSGLGVVDDVQRPGWFVRILDWVYPF"
     gene            32869..34179
                     /locus_tag="PK589_02085"
     CDS             32869..34179
                     /locus_tag="PK589_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNJ18411.1"
                     /translation="MTKPRKQQRAMIMQCFRLMLSSGVLRTPSVDPELLARPWTPPPP
                     KPAPVSEPRQQMSPWFRRTAVGMLAFLTISFFSASLAHAVRVKDVATIEGVRENQLIG
                     YGLVVGLDRTGDQVIGGQFTIQAMMSMLNKMGINLVIDPIQLLTRNIASVMVTAKLPP
                     FVKPGMTLDAIVSSMANAKSLQGGTLLLTPLKAPNQQVFAVAQGPVSIGGFLGGTGGA
                     GGATVTKNHQAAGVVPAGAIVEKELVVDIDQWDTVSVLLRHPDFTTAIRTAEAIDGTF
                     GKGTAVPVNAGLVKTTLPSTFQGRVVEYIATMEGLDVSVDVAAKVVVNERTGTVVLGE
                     HVRLSTCAISHGNLTISVKNTLNVSQPPAPLIGSTQGQTTVTPDVQTDVAEEQSRLIV
                     VDQTVTLGEVVRALNAVGVTPRDLVAILSALRAAGALQANLEIV"
     gene            34197..34526
                     /locus_tag="PK589_02090"
     CDS             34197..34526
                     /locus_tag="PK589_02090"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HNJ18412.1"
                     /translation="MDIKNLLSGQLDLAQMNATQPAGLERVQGLEKAGQKSTEAMHKA
                     GQEFEAYFISYLIGKMRETVPKGLLDRKGEQVWYSFYDQEIAKLATQAGGIGLTAFVD
                     AYVEKNK"
     gene            34627..34932
                     /gene="flgM"
                     /locus_tag="PK589_02095"
     CDS             34627..34932
                     /gene="flgM"
                     /locus_tag="PK589_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNJ18413.1"
                     /translation="MEISHNGRAADLAKILLGVQETDRTQNKKNASQTTPSQDRVQIS
                     ERAKELQRLRAAAEQPDHERDARVGQIQQSVEGGTYNVDGKKVADAIIRNVLTDAVL"
     gene            35008..35451
                     /locus_tag="PK589_02100"
     CDS             35008..35451
                     /locus_tag="PK589_02100"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNJ18414.1"
                     /translation="MSTLSQTTPTESDMLVRGMLDKMLAFQSLLLEEQDAIRSLSFGQ
                     FTSVTTRKSQLLDEIRDFEQRRRQQTSSELSRQDARTTMEAKLLAAIGETDRLNRFNA
                     ALIGQSLEFLQGALKRWQRAPESAALYSSAGTAVPAGAGLVRAKG"
     gene            35463..36857
                     /gene="flgK"
                     /locus_tag="PK589_02105"
     CDS             35463..36857
                     /gene="flgK"
                     /locus_tag="PK589_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNJ18415.1"
                     /translation="MSGLNGLFGVGSNALASFQRAMSVTGQNIANVSTPGYSRQEAVL
                     TETLPENGRPGQIGTGVQVSEIRRSVDSFVEQQLLNSNERIGQFGASQKALSQIQILF
                     NDANDQGIAAGLNEFFKAWQDVATNPADLTARTVLLTKADGLTKVLNQASSQLSTQRI
                     SLDGQIGSAINDINALASKIADLNAQIKLTEVGGQQANDLRDQRGRFLNDLAGLVDIS
                     SIEDGNGQVTVFVGVGQVLVTDHTAFKLTGVPDATNNGLLDVRYDGGTGPNTDITSSI
                     SGGRLKGLLDARDTTATGLQTSLDTLTAQLVSQVNTQHKLGYGLDGSTTQDFFTATGT
                     TAGTISMALTDRQKIAASSTAAGVPGNNVNALALANLQTASVAGLGNTTFQSYYGAMA
                     GSFGATLQGATRDLQGQEILHDQLLAHRAEVSGVSMDEELINMLKYQRAFQAASKLIT
                     TSDEMLQTILSLKR"
     gene            36888..37778
                     /gene="flgL"
                     /locus_tag="PK589_02110"
     CDS             36888..37778
                     /gene="flgL"
                     /locus_tag="PK589_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNJ18416.1"
                     /translation="MRVADQQMYNTLLGNLQRSRLQMLTSQEQVSSQRRVSTPEDDPS
                     SYGQIVLDKSALSQTKQWIRNIDFGTSRVNAADQALGQVQTLITRVRELTIQASSDTT
                     SAEGRQSIAKEVRQLQRQLVQLGNTEVAGQAVFGGTKTNVTPYVITTGDTVTYQGNTE
                     TQSISVGENQTVQILVPGSSIFTGGTTNMFDSLRDLLTALESNNRAGIQTGIGNLDLA
                     TAQISDAQGTVGALANRLQVTHDALDTATLTISKAISDNQDADLATAISQLSLQQVAV
                     QAASQTFSKIFDSSLINYLR"
     gene            37853..38014
                     /gene="csrA"
                     /locus_tag="PK589_02115"
                     /pseudo
     CDS             37853..38014
                     /gene="csrA"
                     /locus_tag="PK589_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
     gene            38108..38587
                     /locus_tag="PK589_02120"
     CDS             38108..38587
                     /locus_tag="PK589_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNJ18417.1"
                     /translation="MKCQSTRFGTFEVNDDTLLVFPSGILGFPDWTKYVMLDHDTDAP
                     FKWLQCVEEPQLAFVILDPALFKPDYQVTITTDALIEIQKQESDEVSIVTILTIPSDD
                     PTAVTANLRGPLVMNHRTRLCKQLVLPEDLPTRYPLFAAASAAPSQSPSQPLQASAR"
     gene            complement(38704..41544)
                     /locus_tag="PK589_02125"
     CDS             complement(38704..41544)
                     /locus_tag="PK589_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ18418.1"
                     /translation="MSRPSHSRSHHPQPPSGFTPSPEMMGLLWEAVPLGICLLAPDHT
                     IVFANRMAARLLGRSPADCAGKRCSELTGQPLELNDSLHSTGVWKFQSGAAAASDETT
                     DMNATDAPALTVIEWEQLRLSGIPSVSTLLTLRDISRECELEQDRDRLATVAEESPCP
                     IVEIDRNGNILYVNPAMVEVLCRFGYDPNGKPDILPDNLPALVATCLLEGRSLTSQEV
                     VRGEACYSWTLCPVPSNQLVRAYGIDLSEVHATHRALNATADHLRESNRQLDQALQQA
                     QAAARAKSSFLAMITHELRTPMNGVIGMASLLLDTSLTEEQRSFTQTIQQCGEAQLSL
                     INDVLECSKIEAGKLELETLDFQLRTTVEDVLSQFAERAQRKGLEITGLVHAAVPNAL
                     RGDPGRLRQVLTNFVGNAVKFTEQGEVTLQAFLESDTPAGVMIKFEVTDSGIGISEEV
                     QGRLFQAFTQADSSTTRKYGGTGLGLAISKQLVELMGGTVGLRSKPGEGTTFWCTALF
                     QKQAVCSPAIVPSAELSGRRILIVDDNESNRTILHHLVSGWGMHDGQARNATEAMHML
                     EQAAANGEPYDAAVLDMLMPGKDGLQLAQDIRAHQHGAGVRLVVLTSLIQPGHAERAR
                     RAGFTAYLTKPVRHDQLQGCLRVVFGIQHKAATASTATGKGQPISPTLITRHTLAEQS
                     PRPRILVAEDNVVNQKLAVRMLDRLGYQPDVVSNGQEAVTAFERESYAAIVMDCQMPT
                     MDGYEATRLIRAQEERPDASRTRAHIPIIALTANALPGDRERCKAAGMDDYVTKPVKT
                     DDLGIILQRWAPVKAAAEVAPPAPARDLSKTDARVFDAGAMLANIGGDAELFDQLIRL
                     FLDRHSVMMKEIESAIGQADAAALERAAHSLKGTAGNLCAPDVVLLASQLEASGRLGT
                     LTEAPSLLVRLDRTVQELVAVLSRQINPTDSQ"
     gene            complement(41547..42797)
                     /locus_tag="PK589_02130"
     CDS             complement(41547..42797)
                     /locus_tag="PK589_02130"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNJ18419.1"
                     /translation="MASTKRIPIDQLTVGMFIAGLDQPWYRTPFLLHKWLVSNPDDIV
                     QLKRHGIQIVTIDTDRGLDVGAAPVAAPESTAPVPEPSPASAESVDASTNGHSSHAAA
                     AAATYREAMEAVERVFTDLEAGQPPKIAALKPVVSKLLKQIIEQPEAMMIQFCLDKMR
                     RFDGTLANHGMDVCVLTLILAVENSCTESDMESLGLGALLHDIGFVRLPRNLYRKTSA
                     LTEQEQVLMRQHPQLAATVLSKIDSLPETVSQIIAQHHEFQDGSGFPKLVKSGGLSPL
                     AQLMALADTYDDLVTSRYGRAPLLPHDAIRQLFVLGAKGRYDKNLVEVAIKMLGVYPL
                     GSLIKLNTNECAVVIGLNHEDRLRPRIRIIRGPDGEIQNPPIDSDLQNQAADQPVRTI
                     LRALDPKQEQIDLPQYLDVAVGGA"
     gene            43004..43717
                     /locus_tag="PK589_02135"
     CDS             43004..43717
                     /locus_tag="PK589_02135"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNJ18420.1"
                     /translation="MSDLIVHRHPASFLEVGLSLQLSLPAEGTGAQYGATVLGWKERS
                     WIVCEWPFHFGQPISCAAGTPCLLRYIYNGKMIGYRTDVLHAQLQPFPFLLLAFPATL
                     DEVPLRKHGRVTAQEPIVLLQADQNLRNQPTGSPCRIGGLLTDLSASGCAVLVQRPMQ
                     DFFPGMVIRAEFEIVGTGHVNNLTGVVRNVSMQADGTHLGLEFRFDGKETIEYRGWGG
                     SVRKALESFVLQKHSFESA"
     gene            43915..45108
                     /locus_tag="PK589_02140"
     CDS             43915..45108
                     /locus_tag="PK589_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNJ18421.1"
                     /translation="MTLHAHSAKLNPRWCLAQDTIDRQDIDHLIDWLRTYPRLTKGAV
                     TVDFEQRWSQWLGRPYSVHCNSGSSANLLMYYALLRSGKLRNTNVIVPSVGWVTSIAP
                     AIQFGFTPFMCEADPDTFGLDLNHLEDLLKRHHAQTVLLVQVLGVPHRMRELQTLKDR
                     YGFYLLEDACAAIGAEYDGKKVGTFGDMASFSFYFGHQMSTIEGGMVSSSDKELTNLL
                     LMLRSHGWSKDVDQARHQELVDQYQVDDFHSPFVFYEPGFNLRSTDLNAFIGLEQLRK
                     LDWMTGRRQANHDRYLEQLGTRFYTQRPPKGSKVASISFGLLAESPEQRKRIVRALVA
                     EGVETRIFSAGNLGLHPFWMNRYGKASFPVADRVHHCGFFLPNHASMTDQDVLHISRI
                     VLEAA"
     gene            45161..46108
                     /locus_tag="PK589_02145"
     CDS             45161..46108
                     /locus_tag="PK589_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ18422.1"
                     /translation="MTTGSTHVLITGGAGYLGSVLTRRLLDRGYAVTVLDNFMYRQNS
                     LMDCCAEEKFQVVRGDCRDERLVTDLLRKADIIIPLAALVGAPLCDRDRVGAYTVNFE
                     AVQLLCKLSSPRQQIIFPVTNSGYGIGQPGVPCTEDSPLRPISLYGETKVKAERVVLD
                     RGNAITLRLATVFGVSPRMRMDLLVNDFVWRAVHDRAVVVFEGHCKRNYIHIRDVVRT
                     FLHAIDHFDDMKNRPYNVGLDDANLSKLELCREIQRVLPQFVSFEAPIGEDPDKRDYI
                     VSNQRLLETGFKPEWSLERGIRELIKCYTVVKAGQYANV"
     gene            46139..47209
                     /locus_tag="PK589_02150"
     CDS             46139..47209
                     /locus_tag="PK589_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HNJ18423.1"
                     /translation="MIISRTPFRISFFGGGTDYPVWFREHGGAVLATTIDKYCYISCR
                     QLPPFFEHRTRIAYSRIELVNNNQEIEHPAVRGVLKYLNIDDGLEIHHDGDLPARTGL
                     GSSSSFTVGLLHTLYALQHIMPSKAQLAQAAIHVEQDVLGEAVGCQDQVLAAHGGLCK
                     ATFFQNGDIGHAPIIMRPERLAAFQNHLQLYFTGFSRIASEVAREQIDRTNQRRSELS
                     TMLQMVDEGVSILTGDRDLADFGSMLHEAWMLKRRLTSRITTPAIDAIYAAAREAGAL
                     GGKLLGAGGGGFMLLFARPEDHERIRLALPGLLQVPFKFEGLGTQIVFYQEDQLMLDD
                     VWAQSSAQTAARLKATLIGKAA"
     gene            47209..47973
                     /locus_tag="PK589_02155"
     CDS             47209..47973
                     /locus_tag="PK589_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNJ18424.1"
                     /translation="MAQFADCDVIILCGGFGTRLQSVVSDRPKPMAEIHGRPFVSLLV
                     EHFLRHGARRFIFSTGHFGEMIEDWFLRHRGAYETLFVRDPVPLGTGGALAHAMPLVR
                     SNPFLVLNGDSFCEIDPERLLRFHTRKRAGATIAVTHDESREDTGAVALGEGDRVLSM
                     VEKPRTRTANYHNAGVYLFDRAVTTLFPDMPVWSLERDLLPRLVTHQCYGFVTASPLH
                     DIGTPERLAHFREIWKDTSAYFPTSLLHQTQGSIAR"
     gene            47970..49190
                     /locus_tag="PK589_02160"
     CDS             47970..49190
                     /locus_tag="PK589_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNJ18425.1"
                     /translation="MTQQRASMPQFKTRPAFSSRGERLIGQEMFRVLDQAQALERQGH
                     RIYHLELGNPRVAPPSQILEATIGELQAKRVGYAPMGGLQELRSAVVERYASLSGKPI
                     AASQVVISPANMLIHQFLDITCNPGERIVLFTPAFPSYWAAAAHLGLDVVPVPLPARD
                     GFSLSRPSIDAALAAKPRAIIVNSANNPTGAVYEPAILHELVARCGQAGIWLLSDETY
                     ADLTFAGSHVTLAAPQAGHVVAMSSFSKVLSVPGFRTGYALAHEAVVAKIALSNSTLY
                     SCLPGFTQSGCLAGLSVLDAYAAEVRARNRRLIATCHDRITHDGVLQCRRPDAAFYLF
                     VDISRTGLDDMTFCRRLLEEEHTAVTPGRCFGDAYASCIRLAVCGQEEDVLEGVSRTI
                     AFAKKLGGCHVQAA"
     gene            49174..50682
                     /locus_tag="PK589_02165"
     CDS             49174..50682
                     /locus_tag="PK589_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ18426.1"
                     /translation="MSKPLDVLLVTPPSRVQVYQELSRDFAAIEPPVWSGLIATYLRQ
                     HSCSVAILDAEAQGLTHQQTAEQIAAIAPRLAVFVIYGQQPSASTQCMPAGRKVCEIL
                     NTLADIPTLVMGTHPSALPKRTLLEEPYTYVCQGEGPATILGLVVALRAPQHSLREIP
                     GLWHLEEGHAVGNVPAPLFSNLDRDLPGQAWDLLDMSRYRAHNWHCFGNPDARTPYAS
                     LQTSLGCPFTCSFCCINAPFTTPMLRVWSPDNVIAQIDRLVREYGVSNIKIPDEMFVL
                     NRRHVMGICDRIIERGYRLNIWAYARVDTVQDEVLEKLARAGFTWLGLGIESGSQHVR
                     DGVEKGRFGEREIVATVERIRAHGIHVAANYIFGLPDDTLESMRATLDLALTLNTEWA
                     NFYCAMAYPGSALYGLAKQHHWTLPDDQGGPGWIGYSQHAYECLPLPTDSLTATQVLD
                     FRDRAFQEYFGHPSYLAMLQRTFGSHVVAHVAEMCRHQVRRRHHDQASHQAA"
     gene            50707..52545
                     /locus_tag="PK589_02170"
     CDS             50707..52545
                     /locus_tag="PK589_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNJ18427.1"
                     /translation="MIKVADFIINTLAERGIDKMFVVYGAANGDLIDAFTRTAATQYV
                     AVMHEQAGGFAAEAYAKVKGIPGVAIATSGPGGMNLLTSMGNCFYDSIPCVFLTGQIN
                     SRFLRPDPSIRQIGFQETDIVAMASPVTKYAKMILNPEDVRFELEKALWMCQEGRPGP
                     VLLDIPLNVQKAMVDAKQLTGFEPPAAMSFDLTVVDQQIARFISDLQQAERPVILVGG
                     GVRLADAQDDVRALGRRLNVPCFPTWNALDVISSDYENYGGRVGTYGGAGRNFGIQNS
                     DLLLSIGSRISGRITGGNVQSFARQAKKYLVEIDPAMVQRKFQQVPFDVNILCDAKAF
                     TQRVLIALDRRSKPVPDFSAWTGRVMEWKRRYDPVRPEFFAQRERVHPYAFMRRLSEK
                     MGATDVLVGDCGGNIVVSNHAFETKYGQRNLTNNGNSPMGFSFAGAMGAWFADPTRQV
                     VCTIGDGGFNMNPQELQTFVNYGVKVKTFILNNHIYGITKAYQETNFQGRAEACGPKG
                     YHPPDFLKVVQAYGIKTVAIANHAEMDAKIDEVLQFDGPVVCDVDMHEFHTYEPRIFG
                     WKTPIEDMYPYLPREEFRANMVIEPAEGWMSPEYPDVVRRTDQSQP"
     gene            52617..54242
                     /locus_tag="PK589_02175"
     CDS             52617..54242
                     /locus_tag="PK589_02175"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ18428.1"
                     /translation="MNVLLLYPTWTGAYGLFGHFARRNSTWPPLNLALLAAIAERHGH
                     NVTILDGESEQIPLEEMVQRAVAMKPDLIGFTATSPFFHLSKTVAEGIKRLAPEIPIA
                     VGGPHITIMKEQALLGCFDYAFIGEAEESWPQFLNAYEQRKDLSTVRGIIYRRNGELV
                     STGQPADITDLDALPIPARHRLPMSRYKLGTLRGRLPFTSIQTMRGCPWKCIFCASEA
                     LKTTEMRVRSPRSVVNEMKQVVEIFGTRHFYIVDDVMTLWKDHILQICDLIDQEGLQI
                     TFEGSTRANLVEEDVIARLVKSGLIRLSFGLETVDPEIRRTMKKQVPLEHYVKANGIC
                     NRYGVEALNSVMIGLPGETRETVRATLNFLRQAREVKQANFAIAVPYPGTEFHKLAVN
                     GEKGVRLMTQDFSEYRRYGSAVTTVGELTPHDLIDLQNEGFVSIYSAPWRWIPMLQKH
                     GMIGGSLMLVRVARLLSKKLFAGHGQSSQEQLISLGDGAFGRIQESAMSSQPVGVAPT
                     GPNSSAPLLTIASSSRAGAAPAGHFGHPTNPNG"
     gene            54264..60800
                     /locus_tag="PK589_02180"
     CDS             54264..60800
                     /locus_tag="PK589_02180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ18429.1"
                     /translation="MTPRPPMKVLLLQLEFPTWKQARAWTYPACFGVAEGLRASGVDC
                     TTLPLIADPPYSSTAWLAHMKRMLKGQRFDQVWVWLVHAPLDEAILNWLSELAPIRVG
                     IIMESLQYSEEDYAWAPHLRARQGFVEQQVRALTHVLVPDECDVERTAARTGVPALWF
                     PPMVPERFISTPQNAPVHRVGVFHGVPYGPREQWIHHPALKSCLTFARPSAPTLFQQL
                     FDRLQQSAIQRLATPAGMPAAELTHYTTMLQEVREGEFREWMTQLPQWAAIVNLPSLA
                     KFFGGRVFEGIAAGRPVVSYAVPGHPMNNSLFVEGEEILYFSPSRPDSLAEVLDRLLA
                     DDTFATRIAGNAQHKLRTYHTSERRLVETLAWIQSGTTPVYGEREAGAAYMLSSAKTG
                     HAAPSVEPRPTQAIGHVDTTIFILTVDDPAYPACKAAVDAQQGGPFHVEIIRNVAPFS
                     AAAQRMIADCRTEFFIQVDEDMILNLDAATKMTDLMRRAPSEVGMICFHLYDEDRACR
                     IQGIKIYRTAAMKPFAFQNVKASEMDLLEQMGQRGVRWVLHPDVLGRHGTVYTPETIY
                     RRYKTMYEKDIRQWNTLTSDIRRKADQFRATGDLLALFALLGAAHGIIEGPRSADREK
                     DARAYGLNELDIFARLFRQTPPASQPYDATRSGTPVANSPLAPEEVRWGGEAAIGNAP
                     DQQAFQRATPAAQPPRHHAARILIVTPYFWPSVGGVEKVAESLAVGLQSRGHAVEVAT
                     YALTERSAQSHRGIPIIDLTFPADFETTSPYSMLEVKRLIESGHYGCCILLGAPLHLM
                     FYAALAIPKDRPVRFILQPTINKEIAESLQGSEQGRTMLSQLGQRVNAVVTFGGNGYD
                     EQFFQERRIPTCVIPNGTPALAPKNDFRQRYGIARDTFLVIHLANLYKVKNHPGLLDA
                     LDHLPNGVQLVLIGNDTHETEYVETVKARLKTRPDVRLLAGLEPADVASALAAADLVV
                     LASHAEVSPLCLLEAMSVGRPWLATPDCGTAAEHAGGLVLPLSQFASTVGLLKKHPEL
                     CAELGRLGREHWAQSHDWATVLTAWEDLIEGRPVSHSFGTPVSIAERRETLREAFRRL
                     LAQEGTAVASTASTLQVETKGVVMDGLNQGPRGSVDRLAISNSAPTPQEQKQQDQFYI
                     DMFVKSRGWSSPGPNHDEAARWSKIAAFLEYLLRRVRQTDPQRQLRMLDVGCGRGWLT
                     NLATAYGTCEGIEPVAGVIEHARRLFPHLRFEAGTAQSVLSRPDFAPYDVILCSEVIE
                     HIPHGRKEGFVAELSMLLKPDGYVILTTPRGEMWEQWKTIAPPCQPVEDWVTEAQLRD
                     IFVSQGFCELGLERVHCELPSLRYIPAPTPADFHKLNLVPIYQIWACQRRGASPVPSF
                     TRPPMVSVIVPTYNRPDRLQVALSSILAQTYQDFEIIVVNDGTVDVSDVIAPLNRDGR
                     ITVIRHDRNRGLAAARNTGIRAAKGTYIAYLDDDDTYLPDHLQTLVTTLQGGEHKVAY
                     TDAWRIHEVRQGDQYVVTGQDIPYSRDFNCIDLLLCNYFPVLCVMHEKACLEQVGVFD
                     ESLFAHEDWDLWIRMATIYPFLHIKHTTAAFTWRRDGSSMTSSTSDTYRRTTEIIYRK
                     YRPYAERIAGVLEAQQKKLEGMRSDAQAKTFDCSIIIPVWNNLALTTQCLTALAEVTQ
                     GVSYEVIVVDNHSTDDTPAFLAGLGGDVKIITNNDNLGFAKACNQGAQAAKGEYLVFL
                     NNDTIPQAGWLSALVEEVKAHSEVAVVGSKLLYEDGTIQHAGVAFSREFLMPYHMYPG
                     VPADAPFVSRRRELQCVTAACMLIRRQVFAQVGGFDEGYKNGFEDVDLCLKVGEKNWK
                     IVYQPQSTVIHLESRTPGRKAHEEENTLRFRERWGRCWWVTDEDRLHFDDGYSVHTHL
                     KDGLLGYTLYLITDPAIRAERAFVADVQRATAVKDQQKIVSLLKRIEQWPTDAWILRW
                     GALLSEGIGHLELALSFWKRVISLKNDPQARLALAKQALESGAMKEADHHVTALLQAE
                     PSHGEGWLLRGILAMQLNAYRDAEQAFEQAKQAGAHPRKAMLGLVMAAMGDNRPEAAW
                     SHVAGLCADYPDDEECMHWMLKCGTLLQRWDALATRLSSFVARNPGNIAMRFALAGVL
                     LRAGRRADAQREYDWLRAMVPTFEGMDELAKQLAESERPLVPNHAA"
     gene            60790..61983
                     /locus_tag="PK589_02185"
     CDS             60790..61983
                     /locus_tag="PK589_02185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNJ18430.1"
                     /translation="MPHEPSPAPRDLVIQMARLGDLVQTLPAIESLQEAYPKRTLDVL
                     CAAPLSSLLAGVSHIGRVIPWDGAQWRDWATGWPENPTVTLQVMQQYLASLGDTTYER
                     VYPLNQHERSHLMTQLFLSHSTREQEHDRMEARILPWAQHLRHVARERGANRVHLADA
                     WCGMCGVLPRGQAPSCEPPPVELPQDLAEIGERQGRWIALVTGAGEADRCIPPAVWSQ
                     WIREFLAQTQDGQVVLIGSGTERETAQAILDSVPTLLHGRVWDATGRTTISQLMRVLQ
                     QCHWVIGADTGPLHLGTLAGARALGWYFSRARMHETGPYGEGHWVYQHATQAQPQRWP
                     IQESIALMCGDQGRRATDWTLWRSGMDRWGAYFDDGSGSATVESSRAEIWRACAPALC
                     ESMAA"
     gene            61980..63797
                     /locus_tag="PK589_02190"
     CDS             61980..63797
                     /locus_tag="PK589_02190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ18431.1"
                     /translation="MNMFQDNLNRLASRHRDLAAAVKSAAGGVLTIEPARTGLPSARR
                     SGRWIHSAYDPLREAQTWADTHAAACKSGEMVVVAGVGLLYHVEALRKKLTTDVSLAV
                     LLPNLDEFHDALVTRSLEGWSESILWLPGTPAEIAETLATIGRPIRCLSYTPAALADA
                     EFHRAVEDALRQGIARQAGGQLTIALVGPIYGGSLPITGYVKRALETLGHKVQLIDHS
                     IHAASYEVMGALKDPRNRQLMQGRMADVLSQWTLATLAESPPDLVLSMAQAPMTLQVL
                     EHLRKKKFLTAMWFVENYRHLTYWQQLAPGYEFWFVFQQGACLDAFRQAGARQVNYLP
                     MAADAELHCPMSLSEEDRQRFGADVSFVGAGYANRRRLFPALLRQPWSFKLWGNEWDG
                     ADDLRSVLQLDGARIDTATCMKVFNATAINLNLHSTTGAGLDPQADFVNPRTFELAAC
                     GAFQLVDHRSQLRQFFTEQEMVSFQNFGDVPGLVQQWLNDHAARQAMANAARAHVLSE
                     HTYVHRMRDLLGHIGLSQPDRVGSVLRGERQQESLVKRCADDRPLEALIQDCPSGQRI
                     ELKDLAARIRSKGPTATLKREELMVLMLDEYRSETRDLL"
     gene            63794..65470
                     /locus_tag="PK589_02195"
     CDS             63794..65470
                     /locus_tag="PK589_02195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNJ18432.1"
                     /translation="MNKRVLLINITRMGDLVQMGTLLQRLQHEWPGAEVDLVVDQRFA
                     PVAKLLPHLRHIVEYDFHRLVDESRAQTKDVVTLYHDMTRWAAPLVEARYDRVVNLTF
                     NRRSGLLTSYVGAKEIRGIAAPKDGDVTIHNPWMAYLTDVHHERRFNHFNLVDIYALG
                     GSGSGPFAPLSLTIPPDTAEWARNFLATQGGQVRSWIAVQVGASDPMKAWRPELFGQT
                     LAALSRQTTAGYVFIGTEEERKAIHLAQTTYRKSGGTGPLCDAVGKTSLPQLAAVLSQ
                     CRLLLTNDTGPMHLAVGVGTPVIDLSVGHVDFRETGPYGPGHWMVQPDLGCAPCGFDQ
                     VCFHHACKDRIVPDQVAALCLHVLAGVPFPRSISGVRIYRSCADEDGLGNTELQAGRE
                     DLLVDWYGRFWRRFWFEEFTGRPSRVPCDHEPSPDCSDALAVLEHLKPLAKKLVSNAE
                     ELVRLTTKRPLPVSALQQMQRLETAERQQIVALSMQSPAAASLAVAMVRDTHNDDGHE
                     LTGLAQSRLATYRRWHKRLQAVSNSFRSFTAAQGSVRRGKTRQLPMARSA"
     gene            65513..66055
                     /locus_tag="PK589_02200"
     CDS             65513..66055
                     /locus_tag="PK589_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18433.1"
                     /translation="MHITLDGEQLQVPEDTSMMNALATLSDKAHAKHHIVTSLTIGGK
                     TISDRDLTPPFLNQPVRDAGAIQAISQSLHTIILEAKQAIERFAAELRSDGQVLLAPL
                     RSGTAQVGTIDAWLGRLADYTEMLEAGQAQGVEGLSSASLLPWIQELLGARTTADAIR
                     VADLLEYELLPRLADARLAA"
     gene            66292..67119
                     /locus_tag="PK589_02205"
     CDS             66292..67119
                     /locus_tag="PK589_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNJ18434.1"
                     /translation="MALVVNTNVASLAAQRNLAVNQAQLGRSVERLSSGLRITRAADD
                     AAGLGVSETLRAQIRSINQANRNAGDGISLTQVADGAAATIGSLLSRMRELATQSASG
                     TLGTTERSYLDQEFVALRSEIDRISTTTEYNGQPLLSGSSNTFEVFIGFKSGSGNSLS
                     VALDDLDVAAVGLTGASVSTATAAQSMLAKIDSAISAVATARANYGSIQSRFEVAIQN
                     LTVTAENFTAAESRIRDADIAQETSVFTKNQILTQSGIAILAQANSLPQQALALLRG"
     gene            67185..67544
                     /locus_tag="PK589_02210"
     CDS             67185..67544
                     /locus_tag="PK589_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNJ18435.1"
                     /translation="MVHDVSNYKDLPAAAIQATQSGPQHTTEKKVEAKAPEPESSTPE
                     TKGKDLEQALTRVREVFKKADSRLEFTVDPDLDRVVVKVMDGDSGTVIRQIPQQEVID
                     LAKRLETPTGLLLHHKV"
     gene            67572..68966
                     /gene="fliD"
                     /locus_tag="PK589_02215"
     CDS             67572..68966
                     /gene="fliD"
                     /locus_tag="PK589_02215"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNJ18436.1"
                     /translation="MAISFGGLGNGVDFGQVVTELAKVQRVPIDALNAKKKDLQTKLT
                     DYGTLGNKLLALQSAANALRLPNSFDRTTTTVSNETKLTAQAGVGAATGSYTVQVTQL
                     AKAHQITNSTAKAVAATTTPIVSGGSGTFTFKVGTGANQTVTLNDGATLDDLRSAIND
                     LGAGVTASVINTGTVSTPAYRLTLTATATGAANAVTVVTDTTSLDFTNTSGTGGTSTL
                     QAGQNAIVVIGEPGQTTISIERESNVISDAIPDVTLTLKSKTVTTPTPEPVTVNITTD
                     PDSVKTNIKALATAYNDIVKFVNERTTYDISTKTGGIFFNESTAKSVLSQLRTALSGE
                     VSGLSTYKSVGTVGFKTERDGTVTVDDAKLDAALASNYSATKALFVTQTTSSGIADRV
                     VKAVDFLDAIDTGAFTVRKTAITSQISKLTDEIGRKEDIASQYEERLRLQFASLDALL
                     QKLKGQTSALQALQ"
     gene            68982..69371
                     /gene="fliS"
                     /locus_tag="PK589_02220"
     CDS             68982..69371
                     /gene="fliS"
                     /locus_tag="PK589_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNJ18437.1"
                     /translation="MIATAANAYQQTQVMTASRVQLIVLLYDSAIQSMELAREAILTN
                     NYKDKARFLDRSMAIVGELSSVLDFERGGEIAVSLHRLYDYMVQQCIQANLRHNGKHL
                     DGPIRCLTTLREGWQVVARQEAVAHVA"
     gene            69361..69702
                     /locus_tag="PK589_02225"
     CDS             69361..69702
                     /locus_tag="PK589_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18438.1"
                     /translation="MSPSSSHPGSPHDRRSIEQLTIQALNAARAGDWDQVDACYSERG
                     LSLAACKPDHTVAQQLLIMDEEIRQAILLAQAGISALLAEASHVTRHLRQLRESSGQL
                     ASDSVTIHREA"
     gene            69760..70380
                     /locus_tag="PK589_02230"
     CDS             69760..70380
                     /locus_tag="PK589_02230"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ18439.1"
                     /translation="MRPSPERPLQRPHHDIKNEERREWLRIDDRLLLEYRRADEPLEA
                     MNQYLPPATEDTIATAVSKPTVDLLVRAGETFAGSPLLPWVSKIDWMLETILKSMVKS
                     HPGSVSIARLTDVDISAGGLGFNTPREFQADDLLMLKVILPPFSMIETMAKIIRVTPL
                     DEGKAGFHLATQFVDLGGDEQELIIRHILQIQAERLRTRKAAHNHL"
     gene            complement(70411..71340)
                     /locus_tag="PK589_02235"
     CDS             complement(70411..71340)
                     /locus_tag="PK589_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNJ18440.1"
                     /translation="MATIVSIGSGKGGVGKSVIAANLAMLLAKRGKRVVLADLDVGGA
                     DAHILFGLLNPPRTLTDFVERRVERLEEVLQPISAHPFLQLLPGTGDTLATANLPYAK
                     KKRLIRHFSQLQADVIIVDIGAGTSYHALDFFLMADCYLTVATPDPTSVLDLYRFIKL
                     AAIRRVLSAFLSRDAVSEALSDRDFASIDEVIQAVGETDPNAKETASNTLQGFQPYLI
                     VNRVSGKSRVNVLQLKKLLKEYVGGDLTMLGEIPDDPAMVRAVRSYLPVVEFEPTAPA
                     SVALNGAADALLAQLTKPATMPQPSAPSPERAA"
     gene            71529..71759
                     /locus_tag="PK589_02240"
     CDS             71529..71759
                     /locus_tag="PK589_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ18441.1"
                     /translation="MNNAIAPTTNVNFDSVSPSEGEILTVSDVARFLRVPKSTVYKLA
                     RLGELPASKIGKHWRFLRRDIHEWMHSRTQQH"
     gene            71865..72728
                     /locus_tag="PK589_02245"
     CDS             71865..72728
                     /locus_tag="PK589_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNJ18442.1"
                     /translation="MAESNAVRKTEMPAPKLSPDTFKQLRDLIYEMTGIHFQDNKTYL
                     LESRLLPRLKACRCQTFESYLNFLRFDAYRDREITELYTVITTNETYFFRDEAQLDSF
                     MKVMIPEVMKTNAQTKQLRIWSAACSTGDEPYTLALLLRDYPPLNGWTIDILATDISE
                     NVLNIARTATYSSHSLRKVPPAMLARYFTGKKEEQTLVQPVKDMVRFMNVNLYDRPRL
                     KLIRGIDIIFCRNCLIYFDEKAKAQIVSDLRDALRPKGYLMIGFSETLHDAAGHFKTI
                     HAGRSVIHEKQ"
     gene            72738..73118
                     /gene="cheY"
                     /locus_tag="PK589_02250"
     CDS             72738..73118
                     /gene="cheY"
                     /locus_tag="PK589_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HNJ18443.1"
                     /translation="MPADPNMKILVVDDMSTMRRIVKNILKQLGFNNLEEAENGQEAL
                     TKLKADTYGFVVSDWNMPVMMGIDMLRAIRADESLKKIPVLMVTAEAQKENLMEAVQA
                     GVSNYVVKPFTAETMQDKINKIFK"
     gene            73145..73795
                     /locus_tag="PK589_02255"
     CDS             73145..73795
                     /locus_tag="PK589_02255"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HNJ18444.1"
                     /translation="MAQPRAAAQSQPATHESEQEEKAPDTKLYEELGELARFIDTTMK
                     TLSEFSTPVSTSTEQLPQALSHLTNLKTMTEQGTHRVMLEVEAIQDNHMKISGMIKDL
                     TLAMQKAQAAPALLQQMQAVGNAINADDKRLLDIMTALSFQDLVAQSVNKLVTILQEV
                     EHKLLQLVVVFGPYQKQAAKKEQDKASEMLKQLEATKNTSMNQDLADEILKQFGFN"
     gene            73804..75696
                     /locus_tag="PK589_02260"
     CDS             73804..75696
                     /locus_tag="PK589_02260"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNJ18445.1"
                     /translation="MSDEMQEILNDFLTESNEMLEVLDQRFVTLESDPNNTDLLNEIF
                     RAMHSMKGSAGFLGFNHLVDVAHRGENILNKLRQGEMAVNPAIISIILETIDVIKAIM
                     ADIRESGTDNHVATASIAAKLDDILNGNTAPAAAPVQTEAVAPPKVEAASSAPAAAAP
                     VAAPQTLGEILVNDGLASKEQVLDALTAQQHQPEPKTPLGEILLQAKAITERALDQAL
                     HKQDKQPKPVEEDATIRVETKRLDSVMNLVGELVLGRNRLIKIGTQLEQQHESDPQVR
                     VLSETLAQLNLVTTDLQLAVMKTRMLPIKKVFAKLPRMVRDLSQKLNKQVHLEMRGEE
                     TELDKSVADEIGDPLVHLVRNAIDHGIETPAERQAKGKAGEGQLTIAASQEGNSIVIR
                     INDDGRGIQVEKVKEKALAKGLISEAELATMEHREILNLIFLPGFSTAEKVTDVSGRG
                     VGMDVVRTNIRKINGSVDLESEPGKGSQIIIKLPLTIAIIQALMVEVERSIFAIPLST
                     VIEAVRISRSEIKTINGREVLHLRDRVLPLIRLAQEFDIPTDANRERFYVVVAALGDR
                     RVGVVVDELRSQEEVVIKSIWDYLETVKGVSGATITGEGKVVLILDTSELVQNAQAWH
                     NPGIAA"
     gene            75782..76765
                     /locus_tag="PK589_02265"
     CDS             75782..76765
                     /locus_tag="PK589_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNJ18446.1"
                     /translation="MSTLINEIDARTRLAGANQMELLLFKLGTNEIFGINVFKVREVM
                     KLPELTQIPEADNRIVGMANIRGIMVPVVGLKRSLGLGMETEGQGNGAPGGNPYLIVS
                     EYNGSLQGFLVAGVDRIIRFSWSAIKTPPAIVRENNKGAVTAVTMLEDGRMVLILDVE
                     KVLNDICPRTDDDVFAGMTIAPELRLKCVLFADDSSVARMQIRKALDRLSMSYVIATT
                     GGEAWQKLQALADQATAEGKEQVDQIQCILSDIEMPEMDGFTLTKHIRADPRLAHLPV
                     MLHSSLTGACNMEKGRTVGATDYITKFDAKMLGEKLALYIQNPNANVKKAA"
     gene            76846..77943
                     /locus_tag="PK589_02270"
     CDS             76846..77943
                     /locus_tag="PK589_02270"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNJ18447.1"
                     /translation="MALPLTQPQSAAKPVRVLVVDDSAFMRKSLTTMLEEGKQIQVVG
                     VARNGEEAVQQVQQLKPDVVTMDVEMPGMTGLQALQQIMTKNPVPVIMVSSVTVEGAQ
                     ETLQALEWGAVDFIPKQLDGVASKIAEIQKILVPKVLAARYAGSKLKRLNVTGAPKLA
                     VPAAKALSSRSVSVTRGTKLIAIGCSTGGPQALFEIVPTIPADCPAGIVIVQHMPSSF
                     TKPFAERLNNLCALEVREAVDGDEVKAGRVLVAPGGMQFRVVKKSITTSVVKLSPNYE
                     RHAHSPSVDIMLQSVAALFGERSIGVILTGMGHDGLEGMKAIKAAGGRTVAQDEASSV
                     VYGMPKAVVEAGCAEKVVSLSKVIGEIMNMV"
     gene            78001..79677
                     /locus_tag="PK589_02275"
     CDS             78001..79677
                     /locus_tag="PK589_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNJ18448.1"
                     /translation="MMKHMSIGPKFIISISAVALVVITLGLVVLYQQEETKIDTLLTG
                     RMNVVSTQIMISRAYITQNYVAKIKKSKAGNEIQVLKDHTGVADAIPFPATAVREMGE
                     EANRTGLYNVRLISQNPMNPSNTPKDNFENEAIRAIMSGAESYARRDEVNGVPTFRRA
                     TVDKATSTACLSCHTNNQVGDVLGMLSMSVPMTEAIALSNRSMWQTGGLMGAIVAIIL
                     VVMYLLLRNIVLQPMAKMSEISRDIAKGEGDLTKRVPAEGHDEIAQMGQYFNEFIEKL
                     QHMIKKVAHVTDKVASASVELSATAEEISKGTDTLTSRASQTAAAVEEMNATVGQVAQ
                     NSGKAASLAQDTVKTAQEGGTVVSSTISGMQQLSEAVSNSATIISDLGKSSDQIGEIV
                     RTIEDIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTTKATKEIGDMIRQ
                     IQQDTRGAVDSMQQGTQKVTAGVDLVNKTGEALSQIVRMVSESADMIRQIAVASEEQS
                     VATQQIASDIENVAKVTKESSSGAHESAKASQDLSQLAVELQGIVGGFKL"
     gene            79705..80223
                     /locus_tag="PK589_02280"
     CDS             79705..80223
                     /locus_tag="PK589_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNJ18449.1"
                     /translation="MSATEQQVQSHIQAAAENSADRLVEKSADDLLQFVICLIGSEEF
                     AVDVLSVQEINRIVEVTRVPKTPPYVEGVINLRGRIIPVLDLRKLFGLTGAQQTTQTR
                     IVVVSVQARLVGLIVDSVEEVLRVPKSAIEPPPSVGTMAGAEFTQGVGRIDDRLLILV
                     DLSRLLLAREAA"
     gene            complement(80277..80519)
                     /locus_tag="PK589_02285"
     CDS             complement(80277..80519)
                     /locus_tag="PK589_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18450.1"
                     /translation="MGPADSLILDAKQAILDEQHRKFQVLQKEGRWTEAMQQFHVTLN
                     CASDVLAESIQLLERVLDARNRRGPSLPDSPDVPQS"
     gene            80650..81420
                     /locus_tag="PK589_02290"
     CDS             80650..81420
                     /locus_tag="PK589_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HNJ18451.1"
                     /translation="MDIATIIGIVVALGSIIGGQALEGGHLGSILQLTAFIIVAGGTF
                     GACCVQNPLSVVIKAATGATLVITNPPHDVKGTITQILDLANVARKQGLLALEGKLKD
                     LHDPFFKKGIQLIVDGTDPKLLQEILEIEVEHHEEEGVHAAKVWEAAGGYAPTVGILG
                     AVLGLIHVMENLADPSKLGGGIAVAFVATVYGVGLANLFFLPIANKMKFKLKEEAGLR
                     TMVIMGLVGLAQGENPRLLQEKLESFLPPHERTKEDKK"
     gene            81504..82277
                     /locus_tag="PK589_02295"
     CDS             81504..82277
                     /locus_tag="PK589_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNJ18452.1"
                     /translation="MAKKKHEEHENHERWLVSYADFITLLFAFFVVMYSVSSVNEGKY
                     RTVSDSIKAALNPVNSTPSSRLPFAVGDSKPKMINTDMSSPNDPVIRRMKEVIKKIHP
                     SLALEMPDIKIIETGNGTIMISLPESILFGSGEARVRPEALPFLKSLAQILIEMDRHI
                     RVLGHTDNVPIRTAQFPSNWELSAVRAVMVVRIFSELNEVPISHLSATGFADSKPIAT
                     NDTPEGRTKNRRVEIIVLEKNLTEESLDSVLPNPGPPAL"
     gene            82403..82633
                     /locus_tag="PK589_02300"
     CDS             82403..82633
                     /locus_tag="PK589_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNJ18453.1"
                     /translation="MTLKPVGDLTIFEVGALCEEVKQAAATHPQVEIDLSEIDKLDAA
                     ALQLLIAVRRSEHCVLTGMTDPIRTRMTELGA"
     gene            82675..83790
                     /locus_tag="PK589_02305"
     CDS             82675..83790
                     /locus_tag="PK589_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNJ18454.1"
                     /translation="MILHWLFLLCAFLAGAFLSWMWCQRRAAAAQQALEAAQQHILKQ
                     HMDRDAIWEQLGHSIIPLVAVLNAQMKSVINQTEQAATDLGQRFSTIASRASFQANES
                     MNLFGDGEMNLDTVLKTTDTMLEGFVSDVMKSSEMALQIATIMDEVERSTKAITGMIG
                     EIEFIADQTRLLALNAAIEAARAGEHGRGFAVVADEVTKLANRSGQAASSINTMVKDV
                     QRSTTQAMQTVESLASVDLSKTLSIKHRLDQMTRNLADRNKTLHSAVMNSKSHSDELS
                     KDVGHIIMSLQFQDITRQKLEHVIEPLTEVRDIMDALIQRHPDDQIAARMNILANLDR
                     SYTMEEERAIFNQSTNGQASGGAQVSASSEDANVTLF"
     gene            83827..>84017
                     /locus_tag="PK589_02310"
     CDS             83827..>84017
                     /locus_tag="PK589_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ18455.1"
                     /translation="MAKTVLVVDDSPTMRQMVAFTLTNAGYQVVEAGNGKEAVGKVNG
                     GAKPDLVVTDLNMPEMDGIT"
BASE COUNT        18320 a        24128 c        24788 g        16781 t
ORIGIN      
        1 ctggctccta ctttgattcc ctcccttcaa ccctctgata gttctaggta tttgtggagc
       61 tggcgagagg aatcgaaccc ccaaccggcg gtttacaaaa ccgctgctct gccaattgag
      121 ctacgccagc ccgcacgagt aactgtcgag agaatcgtgg aagtcccgca gagcggaacc
      181 ctacaattgt tcttcctggc tgtcaagttg cgtttgggca tcttgagtcc gtcgactatc
      241 gtgtaaagca gacgagcgca attttcccgc aaggccttac ttttacttga ataaagtctg
      301 aattcccaat aagctgaacc aacatatgtc taccatgcga atcgctcagt ttcttcctgt
      361 ccagagtcat cttgcatcca gccatctccg agattgcggt cttccagaga ggagtgctcc
      421 cttatgagcc agtcacacat tctcgtgatc gatgatgatc ccgcagtccg ccaactactg
      481 gcggaaacac ttacgagcga aggccatcag gtcacggtca tgtcgagcgg actggatgga
      541 gtggaggcgg tgaaggatca gccggtacac gtcgtactga cggatcttca gatgccgggg
      601 attgacggac tggaaaccat agaccgaatc tcaaaaatcg actccaaagt gattgcgatc
      661 gtgatgaccg gctatggcac ggttgattat gccgtgcggg cgatgaaggc cggcgcgttc
      721 gactttatca ccaaaccgtt tgagcctgac accgttgcgg tcgtggtgcg aaaagcattg
      781 gacgtctaca aacttaaaca ggaaaatcat ctgctacgaa aagcggtacg agaccagtat
      841 cgtcttgaac atttagtggg taccagcgcg cccatgcgga tggtgctgga ttttgtcgag
      901 aaggttgccg atagcgatag caccgtactg atcgaggggg agagcggaac gggaaaggaa
      961 ttgatcgcgc gaatgctgca tttcaatagc atgcgccgag accgtccact ggtgcccgtt
     1021 aattgcgggg cgattcctga aacgctactg gaatcggagc tgttcggcca tgaaaaggga
     1081 gcctttaccg gagcggcgca cacgcggctg ggccgttttg aattggccca tggcggcacc
     1141 atctttttag atgaagtcgg tgaaatgagc ctgccgttgc aggtgaaact gttacgggta
     1201 ttacaggaac gatgcttcga acgagttggc ggaacgagga ctataaacgt ggatgtgcgg
     1261 attattgccg ccaccaatca ggatttggcg caagccgtac aggagcgacg attccggcag
     1321 gacttgtatt accgattgca cgtcattccc attcacattc ctccattacg ggaacggcgc
     1381 agcgatattc cgctcctcgt gaaccacttt atcgctcaat tcaatcaact acgcaggacc
     1441 gagattcttg gcatggagcc ggatgccctg gttcgcatga ccgaagagga atggccggga
     1501 aatatccgtg aacttgagaa catgatcgaa cgtctctgcg tgttgaagaa gaggggaatg
     1561 attacgctgg ccgatcttcc tgaacgtgcg ttgaaaacgg caggcggaaa ggcggtcgag
     1621 gcccccgagc aatttatccg cttctccgaa gatggtatca atctgaccaa agaactcgaa
     1681 cattacgaaa accgtctcat tggggaagcg ctacgcaagg caaatggcat aaccagtcgg
     1741 gccgcgcagt tgctgcaggt caatcggaca acactggtgg aaaaactgaa acggaaaggc
     1801 tttgatccga aaagtcacgg ctactcgatc caaaactgac aaatcttacg acctctcttg
     1861 ccatgtcatt tttttgaccg cttcctcgtt cccgccgttg gtatttctga cacctctctc
     1921 agttttccgc ctcggttcgc ctaaatcatc aagcgatgca gtgtgttacc ggactcctct
     1981 aggggcacag gacttgctga attgccgcct gtgcgaaatt catccaccac agcccgctac
     2041 gtcctcactc ctattgtaag ttggcttcta ctagtaggaa tcgtccccgc cacggccacg
     2101 ggtggccttt ccacgatgcg acaagagctg ccagccatga tggcggagcc atcggagctc
     2161 gttcgtggag cattacctga tccaggtcca cgtttcgagc ggctgccgga aattccggaa
     2221 tggaagcagt tcgaacaggg agtcggtctc taccatcaag ggcaatatct cgaggcacgc
     2281 ctccatttca cgaacatgct tcgtgaccgc cgggagtcgc cgctcagatc gtccatccag
     2341 gcgttcctcg cggaaagtac gcttcagagt cacgaccctg gtgtccgccc actggacatc
     2401 atcgatcagt acaaaacact catgcgcgag aacccgcaat cgacgaatgc caagcgggcc
     2461 gcctggcgca tcggcgatgt ctatcgcatc gaagggtggt tccaggaagc gcagattgcc
     2521 tatcagcatg cgctttccct gtcggaaatc gactcgtttg atgccaaccg ggccatgctc
     2581 ggtctcgcct atgtccagcg aggttttaaa aattggaaag agagcaccca aaccttcgat
     2641 cacgtattga agcggaccgt ggatcccacg ctgctggtct ctgcctcctt gggcgaagcc
     2701 cacagtctct atcggctggg ccggatgaaa gatgccgacg cgctctacga gagtatttcc
     2761 ggccgatggc cggccgcgct gcggagcgat gcctttgcgt tgctgcggta tgctgatacg
     2821 gcaggggagg ccaagcgcgg tccggtcatg cgggagcagt tgctgcattt ctacaatcta
     2881 tatccaaacc ggcctgaaac tccgtttgtg ctgacttacc tggccgatag ttacaaagaa
     2941 gccgggcggt gggctgagtc gagcattttc tacgcagcgt tattaagcca ataccccgag
     3001 gctccagtgg ccgcgacagc ccgccttcga tatgccgatg tgcaggaaca tctggatccg
     3061 gagggagaag agatcaatct tcgacatacc atttcggcgc atctggacca tgttcccttg
     3121 aaggcagggg agatgctcag tcctcgccga ttattcgagc agagtgccaa ggacaatgaa
     3181 ggatcaattg tcggcagcga ggcgctattc cacctcggag aaaccttgga gagggcaggc
     3241 aagccagaag gtgccgtccg tgcgtatgaa caggtggtgt tacgcagtgg aaaattcgac
     3301 aacgatccct ggccggaaaa aggcggcgtg cagctggtcc gtttccttcg ccctcgtctt
     3361 gaagctgcgc ttaaggcaga agacgatttt gaactgatca acatcttcca tcggcacggg
     3421 ccgtttgccg accggttgta tgccggcaca gagctgttgc tcaagattgc agaggcacat
     3481 cgtcgcttag gatttcccgt ggagtccgcg cggctgttcc agtcactggt tcgcgatgca
     3541 aaggcagaga agttccatga agcggcgctc atggggctgg cttacagcta catggaacaa
     3601 aaagatatgc gggccgcccg ctcggtgttt gaacggtacc gcctggaatt tccaaccggc
     3661 cggttttccg gggaggcctt gcgtggaatt ctgaactcgt tcgaggggga aggaaatctc
     3721 gccgccctgc tcaagctggg taaacaatgg ttggctcaca atccgcggca ccatgaccgc
     3781 acgatggtgc agctcaaact cgcggccgcg ttggcgcagt ccaaacaacc cgccgaggcg
     3841 gcggctattt atgacggcct gatcaaagcc ggtcaggaac ttcccgcggc agacctgctg
     3901 cggcatgccg aggtgctggc tcgtctgaat cgccggtcgc aggcactggc catgtacaaa
     3961 gaggctgtcg tcgctggcct tgagctggag caggaggcgt gggcccaact acagatggtt
     4021 caactggcac gcggtgagaa gcgcgaggat ttcgcgaaaa acggtctgcg tgcactgagc
     4081 gtcaatcctg atagtctcgt tcgccgcatg gcagccatgt tggacaatga attgccggaa
     4141 ccaccggtcg acaaacgggg gaaaaaaccg tgatcaggca ggaaggcgac accaccaaca
     4201 acgatttgct gacgcgcgcg tttcgcgatt ttgatcaggc cgccaccgtc ctgcagcagt
     4261 cgtacgatgc gttgacgacg cggctccagc aaatggatct cgagctcgcg caaaccaatg
     4321 ccagtctccg cgagcatctt cgcgaaaccg aggaaatgcg tgcccatgtc acggccgtgt
     4381 tggaatccct ggacaccggc gtcatcgtgg ccgactcaca ggatgtggtg gtgcggtgca
     4441 atcattccac agagcagctg ctgggcgtac cacaggctca actgcgaggg cgccaggcaa
     4501 gcgccctgtt ggcagaaatc cgcaaagacc atggagagta cccgctcgtg ctccccagcg
     4561 gcgtcaccat cgccctctca caaaccgacc tcaccgacga atccgggaac cctatcggaa
     4621 aactggtgtt gatccatgac gtgacgcgca ttcgccagct tgaggatcgg ttgcaacggc
     4681 gcaaccggtt ggaggccatg ggccagatgg tcggcagcat cgcccacgaa attcgaaacc
     4741 cgttaggcag tgtcgagtta tttgcctcca tgttacgcaa ggaccttcgg gaccagcctc
     4801 aactccggag ttatgcggaa catatttcca tggcggttca atccatggat cagctcctct
     4861 cgaacctgct cgtgtatacc cgtcccgact gctcgcgcct gggatggcat gacacggaag
     4921 tactgatacg tgaagtgctg acactggcca gtcacgccat cccgccggac accgtcgacg
     4981 tccgctgcca gatgcatccg cttgtacccc agctctggtg cgacggcccc aagatgaaac
     5041 aggtcctctt aaatttagtc ttgaatgcgg ttcaggccat gccgaagggc gggacgctca
     5101 cgctcacggc ctgtccatcc cctccggaga tccaggaggt accggcggtt catatcacgg
     5161 taagcgacac cggtacggga attccggctg aactgcagtc gagagtattt gatccgttct
     5221 ttaccacgaa ggatcagggg accgggttgg gcctcgccat cgtgcacgcc ctggtcgaag
     5281 cccaccacgg gcggattgat gtggaaagtc gcccgggcca tggcacctcg tttgtgatca
     5341 ccctcccgcg aggaccacga caaccttcgt cgacgcctgt ttcacttgtc accgtgtctg
     5401 ccccttcaga acgttcacgt cacattacga gcatcaccga agaaaagagg tacgaatgag
     5461 cgcgcaacca gccttacaag ggccttccgc ttctgccgat gaggctcgca tggtcttgat
     5521 cgtggatgat gaaccgtcga tgcgaacggc attgtcagaa accgtgcgtc gaatggggta
     5581 tcaagtccgt agcgccatcg acggggcgga tgcgatcgag caggtggaac ggttgaagcc
     5641 ctggttggtg gtgaccgact taaaaatgcc ccgtctgaac gggctcgagc tcgtcaaagc
     5701 cctaaaacag aaagctccgc agacgttcat cattcttatg accgcgtacg gcaccgtcga
     5761 gacggctgtc gaagccatga aatatggcgc gaatgattat attttaaaac cgttctcgac
     5821 cgacctgctc gagcgggtga ttctcaatct tcaagccagc gcagagcctg atgagcagga
     5881 tgcccctgcg gcgctggagg cacgcgcgat actcacgcaa gatcccggca tgattcgtct
     5941 gctcacgacg ctggaagggg tcgccgccag ccaagccacc gtgttgatca gcggcgaaag
     6001 cggcacggga aaagagttac tggcccggta cattcatgcg agaagccccc gtgcccatcg
     6061 acctttcgtg gccctcaact gcgcggcgct tccggacagt ttgctggaga gtgagttgtt
     6121 cggtcatgag cgcggggcgt ttaccggggc gattcagaaa aagctgggca agtttgaaat
     6181 ggcccatacc ggcacgctgt ttctcgatga aatcagcgaa atgaatttgg gcctgcaatc
     6241 gaaactcttg cgagtgttgc aggagaggga agttgatcgc atcggagggc gagatccggt
     6301 cccggtcaac atccgtgtga tcgcgacgac gaatcgctct ctctaccacg aggtgacgca
     6361 agggcgtttc cgagaggacc tgttttaccg tttgaatgta tttcccgtca cggtgcctcc
     6421 gctccgtgaa cgaccgggcg acattccgct gctcgccaga cattttctcc gttcctcggc
     6481 ccaacgcaat ggccttaccg tgccgaccct gtccgagcgc gccatcgcag atcttcagca
     6541 gcgggcatgg aaggggaacg tgcgcgagct ggaaaatgtc atggaacgcg ccattttagt
     6601 gacaacgggg aacgcggttg acgtcgatca cttgatgccg ggggacggaa cggttcccat
     6661 ccaaccagtc gaaaccgtag aaacgctcgt cgcgccctca gcgcacgggt cgctgtggga
     6721 aatggaacgg gatctgatct tcaagacctt ggcgcgagtc aaagagaatc ggacgcatgc
     6781 cgccaaggag ttgggcatca gtatccgcac gttgcgcaat aagttacggg aatatcgtga
     6841 tctgggatat caggtggagg cagaaaaacc ctgacgtctc attcgcccga tgctgggcaa
     6901 aattttccct ctaggtcgta tttgcccggt gccggccgag cgcacattgt cacacagact
     6961 cgggtgaacc cggaaaaatc cccattctga acggggtaca cagattgcac cgttgaacac
     7021 cgaactttct acggagtgtg gaccatgacc atttttgacc ggaccatgca gctgcttgaa
     7081 cgttcattgg atctgcgcgg ggcccgccag caggtgattg cagccaacat tgccaatgag
     7141 gaaaccccga agtatcgcgc cacggacctg aacttcggtc aggctctggc gaatgcgcaa
     7201 cagggcaaac tgccgatcgc cttggtctca acccatcagc agcacatcgg gccgaagggc
     7261 agcgggtttc aacgagtaca gggacgcatt gaagaggttc ctgccggcga tctgcctctc
     7321 gatgccaatt ccgtcaacat cgaactggaa atggccaaga tgtcggacaa cgcccagcag
     7381 tacaacacgt ccgcttccat catcagcatg agattcaaaa cgctccttag tgcgattcgc
     7441 gaaggacggt agtctttcgg ggatacagtg atgcgcttgt tcgagtcatg ggcgaaccat
     7501 aacgaggtgt gtgatggatt tatcagatag tctcgctgtc tccgtatcgg cgctcgatgc
     7561 acaacgacac cggttgaatg tgattgccag caacctggcc aatgcgcaat ccaccaaaac
     7621 cagtaccggt ggcccgtatc gtcggcgtga tgtcgtgttt caggcagccc ccgtgtcctc
     7681 ggcctttcag aaggcgttca cgcaagtgtc caaaggatcg ggccgacatg ccttggatgg
     7741 agtgaaagtc gcccgggtca tcgaagatca gaaaccgggg caaacggtgt atgacccgcg
     7801 tcatcctgat gccgacaaga aggggttcgt cacgatgccg aacgtgaacg tcatggaaga
     7861 aatggtcaat atgatcggcg cgtctcgagc ctatgaggct aacgtacaag ccgtcaatgc
     7921 gacccgtacc atgtggaatc gcgccctgga aatcgggagg taatcatgtc cgatctgcac
     7981 atcgcgggtg ccaatctcat caagccgatc gaagcgcccg ggctccagca agctgggcaa
     8041 accggcgagg caggaggggc caattttttg ggttcgctca aagaagcgat cgggcatatc
     8101 aacgatgcgc aagcaggggc cagtcaggcc gttgagtcgt tggtgaccgg ccaaaatacc
     8161 aacattcatc aaaccatggt ggcgttgcaa caggctgatg cctccttcca gctcatgatg
     8221 caggtgcgaa acaaactcgt gacggcctac gaggaaatcc aacggatgca gatttagcga
     8281 tcggactccc gcgcggatat cacgcataaa ggatttcaca gcacatgttt tcaaaattca
     8341 gccagttcac gatcaaccaa cggttcatca ttctgctcgc cctggccgga tccatcgcgg
     8401 ggctggtggc cgtgacgctc tggacacagc aaccggacat gcaggttctg tttgccaatc
     8461 tggcgaccga cgatgccgcg ggtatcatcg acaaattaaa agacgccaaa gtgccctatg
     8521 agaccaccaa cggcggcacc accatcctcg tgcccaatgc gcaggtgcac gatctacggc
     8581 tggaaatggc cgggcagggg ctcccgcatg gcggcggtgt gggttacgaa atttttgatc
     8641 gcaccactat gggcatgtcg gattttgtgc agaagctgaa ctaccggcga gcgcttcaag
     8701 gagaattggc gcgtacgatc acgcagatgc cggaagtcga acgggctcgt gttcacttag
     8761 ccattcctga gcggcggctg tttgcaactg aacaggatcg cgcacgcgca tcggtcgtcg
     8821 tatctctccg gtccaaccag aacttatcga aagctcagat tcagggcatc gtgcatctcg
     8881 tctccagcag tgtggaagga ttgcaggctc gcgacgtgac cgtggtcgat ggtcacggaa
     8941 atctcctctc caattccagt agcgatgaat cggccggtct gtccggcact caaatggaat
     9001 accagcgcac cttggagaag aacatcgaga cgcgcattca aaccatgctg gaacgcattg
     9061 ttggagtgaa taaggccgtc gtccgggtgt cgagtgtcct cgatttccgg aaaatcgaga
     9121 ccacggaaga acgctatgac ccgaacggcc aagtcgtacg cagcgaacag cgcggacagg
     9181 aaaagtcgag cggcgtcaat gggaccaccg gcggtgtacc cggcgtggaa tcgaatgtgc
     9241 ccggtggggc agaaggcgag gggggacaaa ccagctcgaa caataaccag acgaagaatg
     9301 agacggtgaa ctacgagatc agccggaccg tatcccgcat cgtcgagccg accggcacga
     9361 tcaaaaagct ctcggtcgcg gtgctggttg acgggacgta tgagggcggt ggcaaagcag
     9421 cggagggtgc tgccgagcag ccgaagaagt atgtgcctcg ttcggaagaa gaaatgaagc
     9481 gcatcgaaga aatcgtcaag aaggccatgg gctattccac cgaacgtcaa gatcaggtgg
     9541 aggtggtgag tatccagttc gggcttggag tggaagaacc ggtcggcgct ggtgtcgaag
     9601 ctgcggctga cagcaccagg gcgtggatgc cgtatatccg gtatgccgtg ggcggactgc
     9661 tgttcttcct gattctcttc atggtcgttc ggccgttaat ggtcatgctc gtacagtccg
     9721 ctccggtcga ggcagtcggc gagacgccgg cactgcccgc atcggtcgga caggttgagg
     9781 ccgccatcag cggcaagcag cctggccaga tcctcgacat ggcgaaaaac aaccctgcga
     9841 acaccgccgt cgtggtcaag cagtggctga agagtaacgc gtaacgcgta tggccatcaa
     9901 caaagagctc agcggcgcgc aaaaagctgc cattttactt cgagcgatcg gggaggaggc
     9961 cgccgcggcg gtcatgaagt cgctcgatcc gaaagatatt cggaagctcg gcgagttcat
    10021 gaaagaaacc gcgaatatca cgaagcagga agaagacagc gtgatcgcgg aatttgagca
    10081 ggcgagctct tcaggcgagg tgcaattcga aggacgggaa ttcatggaag ccatcctgaa
    10141 aaaggcgctg ggcccggaaa aggctgcgcg gatgatggaa tccctgaaca ccaaaaccta
    10201 tccggggatc gacgcattga aatgggtcga cgctcgcacg gtcgcgcaga ttctcaagat
    10261 cgaacatccg cagaccattg ctgtctgcct ggggcaaatg gaggcggagc aggcgagcgc
    10321 ggttctggct ctgttgcctg tgcatctgca tgccgacgtc tctcttcggc tggccaccat
    10381 gcaggaagtg cagcctgaag tgctggccga gctcagcgac agtttgcagg aaatcctgtc
    10441 ggcgtcgatg gggatgtcga gcatgtccgt cggaggcgcg gagctgatgg ctgacatcct
    10501 gacccgcgtc gataagaaca ccgaaggcgc catcatggcc aagatcaccg aacgtgatca
    10561 ggccctggcg gaaagcatcc gtgcgttgat gtttgtcttc gatgacctgg tcgatctgga
    10621 ttctcgaggc atgcaagagc tcatgaaaga aatcagcaag gaagacctgc ccatcgcgtt
    10681 gcggggcgcg acgcctgaaa tcaaagacaa gttcctgaag aacatgtcga gccgtgcggc
    10741 agaaatgctg aaagaggata tggagacacg cgggccggtc aaggtgtcgg acgtcgaaaa
    10801 gtcccagcag aatattctca aagtctgccg caaactcgag gaggaaggcc gcatcgtcat
    10861 cggaggcggc ggaggcgagg agttattgta aatggggagc gctgccgcgg gcacgggcgt
    10921 ttcgatggcg gcttcgcccg agtcagaaaa ggaactgtcg ccgcgggccg tcctgatcgt
    10981 cgatgacgag ccctccatct gcttgctgct ggccgagatg ctcgagtcca cggggcatcc
    11041 cgttctcacc gccggctccg ttccggaagc gttgagtcat ctgcgaaccc gctccgttgc
    11101 tgtcgtgatt gtggacgtgc agcttggcgg cgccgatggc atcgcctttc tgcagcaggc
    11161 gctcgacatc gataatcgct tgctcgggat cgtgatgaca ggccacggca acatcgaact
    11221 ggccgtgaga gccatgaaat ccggagcggc ggattttctt accaaaccgt tccaagtcga
    11281 gctggtccgg ctggcggtgt ccagattgct ggaactctac cggttgcgac aggaaaatac
    11341 cgtgctcacc cgcacgttga ttcgctccgg caatattcaa ctccgtcccg tgccgcttgc
    11401 ggatttcagc cgcggcaatc ggccgtttgg aactgaagat cttaccgagt ttgaacgggg
    11461 tgttgcggaa ggggaaaagc gttcgaccga gcgggtggct gcggcgcggc agcgtgagca
    11521 gagcctcgtg gcatccctga tgacacgctt ggaagaaacc tggcaaagcc tgcacgagac
    11581 ggtcgaagaa gaaatcgcgt ccctctcgtt tatgatcgcc cacaaggtgg tgcgggatgt
    11641 cgtgacggaa aagcgcgacc tgatcgtgac gcaagtccgc accgctctcg cacacctgca
    11701 tgaaagcggg ctcgtacgtg tacgggtgca tccgtccgat ctcaccgtgc tcgaatcggc
    11761 acgaacggca ttgagtcaga caccccatgg actgttgaca ttaaaatttg agaccgaccc
    11821 ttccatcagt ccaggagggt gcctcgtcca ggcgcagagc ctgctgatcg atgcgacact
    11881 ggacacccaa ttattgcgac tcggcgaagc cttaagaaag cgggatgccg gtgaagctta
    11941 gcgcacgcct cgaacatgtc gaacccttat cggtcacggg cagggtcgct caggctgtgg
    12001 gcattgtcat tgaagcctca ggaccgttca cctccgtggg agaagtctgt gagatcacac
    12061 gtgagggggg ccacgggtcc gtgatggcgg aagtcgtcgg gttccgcgaa gatcgcgtgt
    12121 tgctgatgcc attgggtgaa atgcagggga tcgggccggg cagccgagtg gtgatgaagg
    12181 gacatgtcgc ctccgtcccc gtcgggccac agatgttagg acgtattttt gacggcctcg
    12241 gccatccctt ggatggcttg ccggctcttc gcagcgatgt gcgtgtgccg ctgtatgcgc
    12301 cgccattgaa tcctttgcat cgggccagaa tcacgcaacc ggtcgatctg ggtattcggg
    12361 ccatcaatgc cttgctgaca tgcgggcaag gtcagaagat cggtgtgttc gccggatccg
    12421 gcgtggggaa aagcgtgctc ctgggcatga tcagccgcta tacgcaggcg gatgtgcggg
    12481 tgattgccct ggtcggggag cgggggcggg aagtcaaaga attcctggaa cgcgatctca
    12541 cgcctgaagc acgggctcgg tccatcgtcg tcgtggcgac atccgaccaa ccaccactcg
    12601 tccgcattcg tggagccctg gtcgccactg ccattgcgga atatttccgc gactgcggca
    12661 aacatgtcct gctcatgatg gactcgcttt cacgattggc ctacagccag cgagaagtcg
    12721 gcctggccat tggtgagccg ccgacgacca agggctatac cccctctgta tttgccgtcc
    12781 tgccgaagct actggagcgg gtcgggccgg cacaaagcgg ggggagcatt accgggctct
    12841 acaccgtgct ggtcgacggc gacgatctca atgatccggt cgcggacgcc gtgcgctcga
    12901 ttcttgacgg acatatcgtg ctgtcgcgtg aactcgccgc gcgtaatcat tttccggcaa
    12961 ttgatattct gcagagcacc agccgggtga tgcgggatat cgtgacgccc gcccattatg
    13021 cggccgcgcg cctggtactc gaacgtacgg cactgtatcg tcgctcggaa gatctgatta
    13081 cccttggcgc atacaagccc ggtacaagca aagatctcga taaggccgtc tcggcccacg
    13141 acggcattac ggcgtttctg cggcaggaga ccaaacaagc ggtcgggttg ccgcaatcca
    13201 tcgacgcact tctggcactg gcagggacga ttcaatgaac gtgactgtgc tccgagccta
    13261 tgcggtgcaa ctggaggaag tcgccaaact ggaattggcg gaactcagtc atgccctgca
    13321 acaaacggtg aatcgaatgg ccatgttgga atcccgagcc aaagaagatg ccgaccggta
    13381 tctgcatcag gttcgtgcag gcagcaccgt ggatcaggtc tacggccatc tcgacgccat
    13441 ggatcaggcc atcgcagcac ggaaagggtt ggaacaggca ttggcgaccg agcaagcgag
    13501 gatggaacag aagcgcgcag aacttctcga agcgatgcag tatcgcaaaa agctcgatct
    13561 cctcgatgcg cgagccgcgc tggagatacg acgccgtcgt gagcgccagg atcagcaatt
    13621 actcgatgaa cgtgcctgga gacgaagccc attgcataag ggagaacggg catgatggag
    13681 acggggagcc gctctgagca gggcaggtgt gttggcgtgg tggcgctgat agcatttggt
    13741 gcggtgttcc tcacgctcat cacgaccgcc catgcgacag aagaagtgaa accggctgct
    13801 gtgccgcaga aagaagagac caagcctgag agcgtgaaaa aagatgtgca aggcccggcg
    13861 accagcgccc cacgtgaaat ctatgaaatg ttggaacaac ggaaacggct cttagacaaa
    13921 cgcgaaacgg ccgtgcgtgg atccgagacg catcttctgg agctcaaggc ggagttggaa
    13981 caaatcgtga cccgccatga gcaagcggtg gaggcggaga agaagcgccg ccaggctgct
    14041 caggccaaag gcgcgggcga agccgaaaag gctaagacga cggccaaagc cactccggct
    14101 gtcaacgtga atcaggccca acttgcgaag atctatgaag ccatgccggc cgaagaagcc
    14161 gcagccagac tggaacgtat gcctgaacgt aaagcgctgg aagtgcttcg gctgctcaaa
    14221 ggcaaatccg cgggcgccat tctcgcggag gtcaagccgg agcgagcagc ccggttgacc
    14281 gaacaattgc tgaccacacc ctagtcattc gtttcacgtc tcgttctcac ggcagctttt
    14341 gccgatcttc aactgcagaa atttccttct ctccattctc gattccctct cggatcacaa
    14401 aacctcgcgt cagaggccat tttcctgcgg cagtctcatg gcatggcctt tgtaatagtc
    14461 tccactgtct attcacggtc tcgtcacaga caggaattgt atggccactg atatgcaacc
    14521 agtcttcaac ggatttccaa gccgctcgtc acaggggatt caggatgcca gggatcgtca
    14581 gccggcggtt cacggttcgg atccatcacg gaacttcgag tcggtgctcg ggcgggcaga
    14641 ggcgcgacag acgccaccca aacacgaggc tcctacgcct cgcagcgggg agaaggcagg
    14701 tctccacgcg cgccaagtca gtcgctctat ctccgactcc tccagccaac catcggagcg
    14761 atcaagcgta gcaccgtccg atgatcactc gtcgaaccgg tgtgaatgtg ccggggagaa
    14821 ggcacagccg tccacagaca aatcagaaga aaagagcacc accaaggaat ccaaaaagaa
    14881 agatcccggg caagacgccg tgtcgcagga catgttgctc gcctccatga tggtgcccac
    14941 tgctcaggcc gctccacctg aacagcagac acctcaagct gaggctcagt cggacagcca
    15001 ctctgagacg tccgtcaacg cggctgcagc catttcaagc gatcgcgccg aagatgggtc
    15061 tgcctcagtc acgactctgg tcgcggcgac atctgaagct ggtgctggta cgcaagaggt
    15121 tcttgcgggt cagtcggatc ctaaagcaga cgcaggcaag gacgcggccc aggctaagga
    15181 acgggcagag aacgggaacg agatggtgca gacaacagcg aaagagctgc catccctcgc
    15241 cggcgacaat tcttcgccgg acgccagagt ggaagaccgc acagactcga ctgcagcggc
    15301 tcggacagat gcggatcgca tgaatcccgc cattgctgcc atgacctcgt cgaagcaaaa
    15361 cccgcacatg gcggaacaag gcccgagtga gggcgcggac cgttcggcga ttcttgatgc
    15421 actggcgcaa ggtgcgtcac tgagcgaggg gcaagcggga tccagcacag gccgatttct
    15481 ggagccggac ggacagaatc tttccgattc atccggcgga actgatcggt cccttcccaa
    15541 ccaggtgccg tcagtcgagc agaatgcccg gccggcattc atggatagga tcaatgacgt
    15601 tcaccaatcg acgccttccg tcaacgacag ttcgtccggt cgaagcgaat ctggccagac
    15661 caccgcagtg ctccgtgctt ctgaatcgga acgggtgggt gagcttcgag ggatggctcc
    15721 agtgtcacag agcgtgacac tggatctgga tccgctagat atggggccgt tgcgggttcg
    15781 ggtcatgatg agcgatcaga cggttcatgc gcatatccga acggaacatg gagagttggg
    15841 tcaggggctg ctgcaacagg gacaatcgtt ggagtcgtcg ttgcgcacgt caggcctcga
    15901 aatgggcatg ttgcgggtca cggtcgacca gcaacaagga cgcggagaca acgcctggat
    15961 gtttcaacaa cagcaacagc agcagaaccg tccatcatca ccaggtctat cccagacggt
    16021 ggcacgagag gatgagcgcg gagcaagggg agagcgtggt gccggatcga acgaacgggt
    16081 gagctttttt gcctgattgg aggaactatg gatatcagta aagtgattac ccagacacct
    16141 gcagcggatc cgtcgacgcc gacgactgag ggacctaaac cgctcggcaa ggacgatttt
    16201 ctcaaattgc tcgtcacgca gctccaacac caggatcccc tgaagccgat ggaaaatgaa
    16261 gcgtttattg cgcagaccgc gcaattctca caactggacc agatgacgaa aatggtgtcc
    16321 ttgatggaga aaagcgtggc gctccaggag tcggcgcaga acaagaccac acagaccggc
    16381 acgacggcag tagcctgatc gaggccggtg tgaatgccgg ccgaatgcag atagcgcacg
    16441 cgacagagta cggcaacaag gaggagagat catgggcatt ctgacttcga tgttcaccgc
    16501 ggtgaccgga ttaagttcat acgggaacgc catgggtgtc atcggcaata acattgccaa
    16561 cgtgggcacg gcgggattca aatcaagccg ggcgaccttt tcagatttga tttcttccag
    16621 catggcaggc ggcgcgggaa gcgatcagat cggcctcggc gtgtacctca acgatgtgca
    16681 aacgaatttt tcccagggat ccatgaccac cacggggaat acgttcgatc tggccatcga
    16741 cggcaacggg ttttacctgg tacgaaatgc ttccggatcg aacttctata cacgggccgg
    16801 acaattcaag gtcgacagcg tggggcaggt ggttgatgca agcggagcgc ttctgcaagg
    16861 ttatcaggcc gatacgctgg gaaacatcac gagtacgatc ggagggatca cattgacctc
    16921 gagtgcggtc ccgccacaat ccaccagtac tgcaaccatt ctcgggaacc tcaatgccaa
    16981 cgccacggca cccacggcgg ccttcgcgac gactgacact acctcataca acttctccac
    17041 agggatgaca ttttatgact cgctgggcaa ttcgcaccag atgcagttgt atttccggaa
    17101 aactgccgcg aatgcctggg gggtctattc ccaaatcgat ggcggagcgg ccaccgcgca
    17161 aaccaatatg acctttaatg ccagcggagc tgtgaccgca ggcggaacac aaaccatcac
    17221 cgccgcgctg acgaatggcg ccacgacgcc acaaagcatc tcggtgaatc tcgcctccct
    17281 cacgcaattc ggtgcagcgt ccggcgtaca aagccagact caaaacggct attcctcagg
    17341 cacgctggat cgcatcagcg tcgataagga aggcaaggtc atggcacagt ttacgaacgg
    17401 gcaaacccgc gccctggcgc aggtggtctt gagccgtttt actaatcctg atggattggc
    17461 caacgtcgga gacaaccact ttctggagac ggtcgagtcc ggtgcagcgc tggccggcgc
    17521 cccgacaatc aatggattgg gccgggttct ttcgggaacc gtggagcaat ccaatgtaga
    17581 cctcggcaaa gagtttgtgg acatgatcat tacccagcgc gcctttcagg cgaactcccg
    17641 agccatcacg accagcgacg aaatgttgca ggaactcgtg aatctcaagc ggtaagcggt
    17701 gagggccccg gcgcggtgca atcccgtcgc cggggctcaa ctcggcaatt tctacctagt
    17761 tgaagcgctc atcctcgcgg ctcttcgtct tctgcctctc gtcaacattc tgtcggcccc
    17821 cagagatccc gcgtcactac cgtgacaaca cgtatgccgc tcaggcgacg tgtcctcgcg
    17881 cgggagagtg actaactcca aatcattgtc ttatcacgcg atctgcgacc tgtgttgtga
    17941 caggcgggag ggctgctctg tgcggcacgt ggcatatgca ttgcacaact gaccggcacc
    18001 ggcaaacact gtccgcagga gggttgtatg tcagaagcag ctgaagaaaa ggccccagca
    18061 gcaccggcag gtattcccat gaagatggtc atcatcatcg tcgggggagt gttgatcctc
    18121 gcgttgggcg gcgcctttgc gatgatgaaa atgatgggcg ggtcatccgc cgaaaagacg
    18181 caggccgagg gtgggggaga aaaggcggct gcgcatggcg ccgctgaaga gaagtctgga
    18241 ggcgagcatg gggccgtcgc tgatgcgagc gccatcgccg atctggatcc cttcatcgtc
    18301 aacctcgcgg attcaccgga aattcggtac ctcaaagtca ccatgaaggt ggaagccgac
    18361 aatgcgggca ccgtcgaaca aatcaaagcg cggacaccgc aaattcgaga tgcgattctt
    18421 gtgttgttga ccagcttgga ttcggctacc gcgcgttctc cccagggaaa gcataagttg
    18481 cgcgacgaca tcaccgaacg gatcaacggc ctgttgccca agaaatcggt caagtcgacg
    18541 tacttcaccg aattcgtgat tcagtaacca cgcgacgtca gcaacatcat ggacaagata
    18601 ctctcccagg aagaaatcga tgccttgatg ggcggagtca tgtccggcga cgtcgaaacg
    18661 gcgccgaagg aggaggaaga accaggggga gtcaaaacct acagtctgac gagtcaggag
    18721 cggatcattc gcgggcgcat gcccacgatg gagatgatca atgaccgctt cacgcgccag
    18781 caatctgtct cctggaccag cgtgctgcgc gagaagatcg aattcagcgt cctcggcacg
    18841 cagatcatca agttcggaga cttcatccag aaggtcccgg tgccgtcctc cttcaatctg
    18901 tttgccatgg agccgttgcg cggcaacggg ctctacatca tggacgccat gctggtgtac
    18961 ctgatcgtgg attttttctt cggcggaaaa gtgcagacgc acgtgaagcc ggaggggcga
    19021 gaattcacga cgattcagtt gcggttgatc aagaaactcg ttcagcaggc gttggtcgac
    19081 ctggagaagg catggcaggc ggtcatgccc gtgaagctca actatctgcg ctcggagagc
    19141 aaccctcaat tcgccatggt cgtcaccacg tcggaaattg tcgtggtggt cacgctccag
    19201 gtgcatctcg gcgacatcac ccgcgaaatg tttatcgcgt atccctattc catgctggag
    19261 cccatcaagg aaaagctcta ctcgggcctc tttgccgaca acgtggagca ggacagcagt
    19321 tggggaagcc gctttaagga ttcgctgcag gagtgtgacg tggcagtgac ggttcaactg
    19381 gggacagcca aaatcaacgt ggaagacctg atgaacttta gtcctggcga tgtgctgatg
    19441 ctcgatcaac atccgggcga tccgatgctg tgtctggtag agggcgatcc gaaattccac
    19501 gggcagcccg gtgtattcaa aggcaatcat gcctgccgcg ttaccaaagt gttgagttag
    19561 gtcgcgccgg aagtaaagga gatttgcgca tgggcaatgg cgatacaacc ccgcaggcgg
    19621 atgcctccag cggcgcggac atgagcccgc aggcgacctc atttcccccg gtcgataacc
    19681 agggtgcggt accggccaac aaaaatatcg atttcgtgct cgacattccg ctgcaggtaa
    19741 cggtgcagat cggctcgaca cgcatgctga ttcgggagtt gttacagctc ggacaggggt
    19801 ctgtcatcga gctgaacaag ctggccggag agccgatgga agtgctggtc aataacaagt
    19861 tggtggcgcg cggcgaggtc gtggtggtca acgagaagtt cggcattcgc ttgacggatg
    19921 tggtgagccc caaccagcgc attcagcaac tcggataggc gccgcgtcat tcggcgagac
    19981 cgccggtgcg agcgagcaga aagtcgatcg ttccatggag atttgggaaa gtgtggtccg
    20041 catggtctcg gcgctgggca ttgtcctggc gttgattctg ggactgttgg ccatcgtgaa
    20101 atccagggtc ggccagcgct ttcttccggt cggcggcgct ccattgatca aagtgctggg
    20161 cagcagttcg ctgggagcgc gcaagcaggt catgctggtt gccgtcgccg gcgaggttct
    20221 gattctcggt accacgccaa cggatctggt gtcgcttgga aaagtcaccg acccggagca
    20281 ggtaaagcga ctgttgcttg aagcctcctc gaatatggct ctctccccgg cgcaggctgt
    20341 cgggtctgaa taccgcgagg agacgttaca tgccggacga taacctcaca tctctgcagc
    20401 aggcaaaaaa gggaggcgca ggaaccgtcc cccgatgcat gggatgggtc ggtctcgtcc
    20461 tgctcgtggt tctggccggc gcgtcatcgg ttcacgcaga ggggccatcg ctgacgcttg
    20521 atctcggcca gagcgggacc aaacagaccg ccgtggtgct gcagattctt gcgctgttga
    20581 ccgtcctgtc actggcgccc gctttgttca tcatggtgac ctcgttcacg cgcatggtga
    20641 tcgtgctctc cttccttcgc caggctctcg gaacgcaaca ggtgccgccc aatcaggtgc
    20701 tgatcagttt ggccctcttt ctcaccatgt ttgtgatggc tccggtgggg caggcggtct
    20761 acaaggatgc gctgcagccg ctgcttgcgg aacaaatcgg ctacgaagat gcctggaatc
    20821 gcggtattca accgatccgc ggcttcatgt tgaagcagat gcgggacaag gatctggaac
    20881 ttttcctgga attgtcccat tcgcccaaac cggcgcaagt agatcaggtt ccgacccatg
    20941 tgatcattcc ggcgtttgtc ctcagtgagt tgcgcatttc gtttcagatc ggcttcctcc
    21001 tctacattcc ctttctcatc gtcgatatgg tcgtggccag cgtcctgatg tccatgggca
    21061 tgatgctgct gccacccgtc atgatttctc ttccgtttaa gctgattctc ttcgtcctgg
    21121 ccgacggctg gtatctcgtg gtgggctcca tggtgaagag ctttcaatag gatggctgca
    21181 catgacgatt gaaacggtca cggaaatcgg acgtcaagcg atcgaaaccg ccatgctggt
    21241 gtcggcaccg gttttaggcc tgagcctcgt ggtcggcttg atggtcagta cctttcaggc
    21301 gatgacgcag atcaatgagg cgacactcac gttcgtcccg aaggtgttgg ccgtctttgc
    21361 cgcgacgctg ttgtttctgc cgtggatgat gggcgtgctc atcgcattta tgactcatat
    21421 catcaccagc attccgacgt tgattcactg ataggtcgtc cacagcatga acgtcgcgca
    21481 aacgttgcac ctcgcattgc cacagtttca gtcgttttcg attctgttgg tcaggattgc
    21541 cggtatcatc agcgtctttc ccattctgaa cacgttgacc atcccgatgc cggtcaaggc
    21601 ggggttagtg acaatgctgg ggctcgtgct ggctccgatc attcaattgc ccagttttcc
    21661 gaacgatccc gccctggtgg tggcgggcat cggcagcgaa ttcctgatcg gcctgaccat
    21721 cggcctggcc gtccgtctct tgttttccgg ctttcaggtg gccggagaat tggtcggcac
    21781 gcagatgggg ttcagcgcga tccagatgtt tgatccgatg agccaccaaa acgcacccat
    21841 catcgcgcaa ttccaactga cgctcggctc gttggtcttt ctttcgctca atgcacacct
    21901 gatggtcgtg catgccatcg gtatgagtta cgaatttgtg ccgcccttcg gcgccaaact
    21961 gtccgacagc atcgccacgg acattattca tttagcccag aacatgctgg gcgtcgcact
    22021 caagatggcg gcgccggtct ttgccaccct gctgatcgtc aatacggtcc tcgccatcct
    22081 tggtcgcgcc gtgccgcaga tgaacgtctt tgtcctgagc ttcccgatca cgatcggggc
    22141 gggcctgttc gccatgagtt tggccatgcc ctttacgctg tcgctgtttg agaaagaatt
    22201 cgagaacctg gtggagacga tcttgagtct cctgaagggg ttggggcatg gctgacagcg
    22261 cccaaaatcg cacagaacag gccacaccga aacgcaaagc agaggcgaga tcgaaaggtc
    22321 aaatcgccct cagccgcgat gccgccatgg ccgtcgcttt attgggaagt ttcgcggcgc
    22381 tctactggat gacgcctggc attatggagc gactccgcca ttccttgcag cgctggctca
    22441 gccaatccat ggaggactcg acacttcggg cactcagtct cgaccacctg catttgattc
    22501 tccgccagat cggaatcgac gtgtttgtga tgctgggacc ggtagtggcg ggaatcgctg
    22561 tcgtaggtgt gggcgccaac ttgatgcaaa ccggcttcct gtggagaaaa gacggcctca
    22621 gtattgattg gtcgcgtatc agtccggtgg gaggcttttc gcggctgttt tcggtgcgcg
    22681 cattgagcga actggtgaag tcctggctca agatcctcgc gatcggcagc gtcggctacc
    22741 tcacaatcaa agaggatatg ctccaatttt tgccgctaac ccagttcggc atggaaaccc
    22801 tgctgcccac ggtggggtgg gcgaccttca aggccgcact catgatgagc ggcgccgctc
    22861 tggtcatcgg tgccctcgac tacgggtatc aacgctttga atgggaacgt ggtctgcgaa
    22921 tgtcgcgcga cgaaatcaag gaagagagtc gtgccgcgga aggagatccc gggctcaaag
    22981 cgaagattcg cagcacccag cgcgagatgt ctcgcaagcg catgatggcc gcggtgccca
    23041 cggcggacgt catcatcacg aacccgaccc acctggcagt cgcattgaaa tacgattcaa
    23101 aggcgatggg cgcaccgatt gtcgtggcga agggcgctgg attcatcgcg gaaaaaattc
    23161 gggaaatcgg tcgtcagcat ggcgtcatga tcgtcgaaaa caagctggtg gccaggacgt
    23221 tgtacaagtt ggtcgaagtg ggccgggaag tgccggaaga cctctatcgt gccgtggccg
    23281 agattctggc ctttgtgtat cgcgtacgtg gcaaattgcc gccggcatag tcagccggta
    23341 gggagcggag atggcgaagg acaatacctc gatacccagc acatccctcg tgaagcatcc
    23401 ggacattctg atgtccgtgg gcgtggtcgg cattctgatg gtgatgctcg tcccgctgcc
    23461 gcgcttcttt ctggacttac tgctgagctt caatatcacc ctgtcgatca tcattctcct
    23521 ggtcggcatg caggtccggc gtccgctgga gttttcggtc ttcccctcca ttcttctgat
    23581 ggtcaccttg ctgcggctgg cgctcaacat cgcatcgacc cgcctgattc tgttgcatgg
    23641 caatgaaggc gcggccgcgg cgggcgaagt cattcgcgca ttcgggaatt tcgtcgtcgg
    23701 cggaaactat accgtcggtc tggtcgtctt tacgattttg gtgatcatca actttgtcgt
    23761 catcaccaaa ggtgcgggac gcgtggcgga ggtcgccgca cggtttacct tggatgccat
    23821 gcccggcaaa caaatggcca tcgatgccga tctgaatgcc ggcctcatca atgacaagga
    23881 agcccgccag cggcgaaagg aaattgccca ggaagcagac ttttacggag ccatggacgg
    23941 ttcgagcaag ttcgtccgtg gcgatgccgt ggcggcagtg gtcattacgc tcgtgaatat
    24001 tttgggtggc ttgacgatcg gcgtgctgca acagggaatg acggtagcgg cggccgcaca
    24061 gacgtacacc ttgctgacgg tcggcgaagg cctcgtcgcg caagtgccgg cgctcatcgt
    24121 ctccaccgcc gccggtatcg tcatcacccg cgcagcctcc gaggtcaacc tcggattcga
    24181 aatcactcgc caggtcctga tctcgccgaa agccatcggg acagcttccg ggattctcct
    24241 cgccatggga ttggtgccgg gattacccca tctggccttt ctggcgctcg gtagtttgac
    24301 cggctggatg gccttccaac tcaatgagca ccagaagacg gcggtcgcaa ctcccgaacc
    24361 ggaatcccct caggtcaaag ccgaggaagc cgctacccag gtcgtgccgc tggacctcat
    24421 ggaagtccag gtcggatacg gactcatcgg tctggtcgat ggagggcagg gtggcgcctt
    24481 gctcgaccgg atcaaaggct tacgacgtca atttgccgaa caaatgggat tcgttctgcc
    24541 gccgatccac attcgagaca atctgcaact caggcccaac gaatatgccg cactcctcaa
    24601 gggcgttgag ctggctaagt cggaggtcat gccggcccat gtgttggcta tcgatccggg
    24661 gaccgctcaa cgtggaatca ttcatgggtt gccgaccaag gaacctgcgt tcggacttcc
    24721 agccctctgg gttcccgagc aacaacgtga acaggcgcag atggccggat acacggtcgt
    24781 ggatcccggg tccgccatcg cgacacatct gtcggagctc atcaagcgac atgcccatga
    24841 gttgctgggg cggcaggaag tgcagggctt attggatcag ctaggcaaga atcaccctaa
    24901 gctggtcgag gaagtggttc cgaacttgat tcctctcggg acgctggtac gcgtgttgtc
    24961 gcatctcctg cgagaaggcg tgcccattcg cgacttgcga acgatcctgg aaaccctcgc
    25021 cgaccatgcg gcgagcacaa aagacgcgga tattctcacg gaagtcgcgc gtcaggccct
    25081 gggacggacg attacgaaac aatatctcgc gccggacggc tcgttgccga tcatcggcct
    25141 cgacccgcgc ctggatcgca cccttgccga tcaggccaat gcagtcgggc agggaaatca
    25201 atggatcccc gatcctatgg tggcacagaa actgctcagt gcattgaagc aggccgcgga
    25261 gcgcatggtc ggaagaggac atcaaccggt gattctttgt tccccttcac tacgtaggca
    25321 tctgcggaaa ttgacggacc gcattttgca ctcggtaccg atcctgggat tgaacgaagt
    25381 ggatagcgtg acgcgattac aggctatgga aacagttcgt ctcgatctcg acggcggtga
    25441 cacgaggagc ttggcatgaa ggtacggacc tttcacgttg catcaatgca tgaggccatt
    25501 cgctccatca aggatacgct ggggccggat gccgtcattt tgtcgacgaa gcgcgtgcga
    25561 tcctgggata acgggttcgg cctcctgggc ggatcgatgc tcgaagtgat ggcggcgatt
    25621 gaagacgatg ccgcggtgcc ggaatctgca cccatactgg gcgaagatga acgttccgct
    25681 ccggctgtcg cacaagctcc cgcggtgccg gccgaggagt cacggttcca gaaaacactt
    25741 caaggcgcca tagagaagag agaggaccag ccgcgccctg cttcccgcca ggcctctttt
    25801 gttcctcaag aactttcccg cacggcaaag tcttcccccg tcaccaagga cggtcagggc
    25861 acaaccttcc aatcattcca acgggtctat gaagacctcc tggcccaagg cgtggagcat
    25921 gccactgctg aagcctgttt gcgagagttg cgcgagacgt tggttgaaca ggggacctcc
    25981 cagcgcaatt cttctctcca gcttttgcgg acggtactgc tcgatcgtgt ctcgaccgcc
    26041 ggtccgttgc tgagcaacgc cggcgagcaa aagatcgcga tgtttgtcgg gcccagcggc
    26101 gtcggcaaaa cttcaacgat cgcgaagctg gcgacgcact acggcatcgt tgaacagcgg
    26161 agtgtcgccc tcatcaccat ggacacctat cgtcccgcgg cggtggaaca cctccggctg
    26221 tacgccgagg tcctgggaat cgagctggcg gtcgcatcgt catgcgagga cgctcgcgcc
    26281 gctatcgcgc aagcgcgtga agcagagttg atcctgatcg atacggcggg attcaacccc
    26341 tatgaaccga tcgcggcgca gcggtggcga gggttgctga acggggccct tcccatggaa
    26401 gtccatctcg tgttggccgc cggcacgcgt gtgccggata tcgtggtcag taccagccag
    26461 tgtgtggacg tgccctcgtt gcgactgctg tttaccaagc tggatgaaac gaccgggtac
    26521 ggcggaattt ttgaagccac gcatcggacg ggcatcccgc tgtcttattg gggcaccggt
    26581 caacgtgtgc ccgacgactt agtccaggcg caagtcaacc gcttgggcga cctgctgctg
    26641 ggtgggcagg ttcggacgcc gggagagcag aatggccagc catggatcag gcaggccgca
    26701 ccggcagttg tgccaggcat caagacctac acgcgataga cggtagcgaa taacagggga
    26761 gagacgatgg cgatcgaacc agacattttg gatccgcaac tgaacacgtt tgacggcagt
    26821 gtcggtccct cccgcacgca ggtcatcacc gtcacgagcg gaaagggcgg ggtagggaag
    26881 accaatgtgg tggccaacac cgccatcgcc ctcgcccaga ccgggaaacg cgtgctggtg
    26941 ttagatgccg atcttggctt ggggaatgtc gatattctcc tggggctgac gcccaaatac
    27001 acgctggaac atgtcctggc gaaaacgtgc cggctcgagg agatcgcctt gaccggtccc
    27061 aacgggatcg tggtgcttcc tgccagcacc ggcatttcgc aactgactgt cttgaccgaa
    27121 gcgcagcagc ttattctgca ggaagagctg gaacgcctgg cctccacgat ggacgtgctg
    27181 ttgatcgata ccggcgccgg catctcgtca acggtgacgt acttcgcggc tgctgcccag
    27241 tccatcgtgg tcgtcgtgtc tccggagccc acgtcgctga cggacgccta tgcgttgatg
    27301 aaggtcttgc tgcggcaata ccgtgaacgg cgctttcacg tcctcgtcaa tatggtgaag
    27361 tcaccgcgtg acgccgcacg catctaccgt aagctggaag tggccgtcag ccgctttcta
    27421 cacatttctc tcgactacct gggcgccatc ccgcaggacg actatgtgcc gatggcggtg
    27481 tcccagcagc gtgccgtcat cgatctgttt cctcacgcgc cggccagccg cgcattccga
    27541 gagctgactg aagcggtggc ccagttgacg cctccggcgc ttccgaaggg cacagtgcag
    27601 ttcctctggc aacaactctt gcgaacgcac tgactgaaag gactgaactc gcatgatgga
    27661 agcacgaaaa atgtctgcga ccggaaatgc ctcgcgccgg gtgattgcgg aagcggatcg
    27721 tgagcgggtc attcaggaat tcacgcatgt cgtgaaggcc atggcccatc ggttggcttt
    27781 ccggctgccg gcctacatgg atgcggagga cctgatgtcc gtgggcatca tggggctcat
    27841 ggatgcgatg gacaaatacg atccgacccg cgaggccaag ttcaaaacct atgcggaatt
    27901 ccggattcgg ggcgccatgc ttgatgaaat ccgatcgatg gattggattc cgcgctcggt
    27961 tcatgaacgc atttccctgc ttcaaaaaac ctactccgag ctgttgcatc gattcggtcg
    28021 tccgccaaca gaccaggaag tcggcaaaga actgaaaatg tctgcggacg agttggacga
    28081 gttcctgacc cgctcgcgag gcgccgtggt catcagcctg gatgacctcg gagtccagga
    28141 tgcggatgga cacaagatca tcagcatgtt gaccgactcc aatcagccgg atccgctctc
    28201 agtgctggtg aatgagcgag cccgccacgt gctggaagcc gcgatccagg acttgcctga
    28261 aaaggagcgc ctggtgctgt cactctacta tttcgaggag ttgaccatga aagagatcgg
    28321 gcaggcctta aaagtcacgg aatcgcgtgt ctgccaaatc cattcgaaag ccattcttca
    28381 tcttaaggcc cggcttgaac cggttgatgt ctagatgtca aaccggccat tcgcatccac
    28441 tgctcctcct tgttgtcggt tctatgaacg atcttcagtg acaccgaccc accgccttta
    28501 gtttcccccc gctccgtccg atacgctcta aaccccaata gccattgggc aacctatcag
    28561 ttctcttcgc gcgtatgagc gtgcctccat cagtaaccgg cgagtcgagc gctgccgttg
    28621 agcctgagcc cgaccatcat catgcctggt cggacaatga cgcgctcacc cgatcctatg
    28681 atgccgcccg attcggcgta ttggcctgtc tcctgtttat ggcaggcggc ttgtattgga
    28741 ccgcggccat tggtctcctg accatctccg cattaacggc ctatcccgtg atggtttcga
    28801 tcggcgtcgc cttcacgctg atgattttcg gggcgacctt ctggactccg cgaagtcgct
    28861 cttccgctgt gcggccgctc tggagcgccg cagaaagtgg cagcaagacc acgtacgatc
    28921 cggtcaccgg cctccccctg aatcgtctgt ttctctcgct cctgaatcaa gccctgatcc
    28981 gtggacagaa gcatggacgc caggtggcgg tcctcatcat cgagctggat tatttcacgc
    29041 ccgccgccga aagccctgca gaactgaacc gtcatctcat gtaccggatc gaagccgccc
    29101 gcgtgaaaag cgccttgcgc acgaccgaca ctgtggcgcg aatagcggaa cggacgtttg
    29161 ccgtgttagt ggatcagatc accgatgcgg ataacctgtt gctgctcgcc aggaaaatgc
    29221 aaggcgccat cgccttgccg attaccctcg atcatcatga agtgttcctc accggccgga
    29281 ttggtctcgc gctttccaag cgggacgatc ttgaccccaa tgccttgctt gaggctgctg
    29341 cacgagccct tgagatggcg cggaccgacg cgtcgggaat gtccggcatc gagacggcgc
    29401 tccctcagcc acagacggat tccacctcca cgattgcggc ctaattctcc ctcactcatc
    29461 ctcggcacct gctcaggtag gcaggacttt cccagcctgc ggtaaagact tccactcgcc
    29521 agcatctttg acagctctcg tcgaccgcac cgggctgcgc ctgcgcgcgt ttacgcagca
    29581 caccgtcgcc atctatcggc aatcatgagt gattttctca gtctgcggta gcggtgcgag
    29641 cgatgcagcg cttcccgcag caatggcatg tcgattgcta actctccctg tgaacagaga
    29701 gaagggttgg aaccgacacc atatgaatcg agctatctat cccatacttt ccggcgcagt
    29761 cgcccaagaa aaacagctcc aggtattcgc caacaatttg gcgaacgtca atacagccgg
    29821 attcaaacag gaccaacagg ggtttcgcgg cctcctggcc cgtggcgggt cggtgggtat
    29881 gggcgccgca tccagcggca tcacctcgac gatatcaacc agaccggccg gccccaccga
    29941 gcgcgtgttt accgaagcaa acggcatgcg tacgaccttc gagccgggac ggatccgcat
    30001 caccggcaat ccgctcgacc tggccattca gaccgacgga ttttttgagg tcaagacgcc
    30061 tgacggcgtg cgctataccc gcaacggaat attctccctc gatggccaac gacgtttggt
    30121 caccaatctg ggacatccgg tcatgggcac gaaaggggag atcaaagtgc cgcccggcaa
    30181 cattcagatc aacgccgagg gcggcatcca agtcgatggc aacgcgatcg gcacgatcaa
    30241 ggtcgtcgaa tttcccccta acgagatgcc gcagaaattc gcggaaggct tgttcaccgg
    30301 cgggaagccc agcgtgtcga agaaaccgca gctccagtcc ggccatatcg aagaatcgaa
    30361 cgtcaattcg ttgagcgaaa tggtgaagat gattcagggc atgcggagtt atgaatcggc
    30421 gcagaagctc attcagacgc tggaccatat gacggatacg gccatccagg atctcggccg
    30481 ggtgcaatag gaggagttgt atgattcgtg caatgtggac ggccgccacc ggaatgacgg
    30541 cccagcaact caacgtcgac acgatcgcca acaacctggc caacgtgaac acgaatgcgt
    30601 tcaagcgcag ccgtgcggaa ttcggcgacc tgctgtatca gattcaacgg ttgcccggca
    30661 cgaacgcgtc gaacgtcggc gtgtttcccg tgggcgtaca ggtcggcggc ggcgtccgtc
    30721 ccattacggt tgccaaggaa tggatgcagg gcaacatgcg acagacgagc aacgacctcg
    30781 atctggccat cgacggcgca ggatttttcc aagttgcgcg tccggacggc acgatcatgt
    30841 atacgcgtaa cggatccttc aagcgcgata acgtcggcaa tctggtcacc ggcgacggtg
    30901 atcagttgac cccggtcatt accatccctt ccggcgcgct caaaatcgat atcgggcagg
    30961 acggcaccgt ctccgtcttg ctccccggtg tgacccaggc gtcgcaggtc ggacagattc
    31021 agctcgtgcg tttcgacaat ccctccggtc ttgtcgcaat gggcggcaat ttgttcctcg
    31081 acagtttcgc gtcgggaccg gcgcagcagg gaaccggcgg attttccacc ggcttcggca
    31141 cgttgcagca gggctttctg gaaagttcga acgtcaatct ggcggaagag atggtcaaca
    31201 tgatcatcgc ccagcgcagt tacgagatta actcgaagac gatccaagcg tccgacgaaa
    31261 tgatgcagat cgcgaataac ctgcgccggt aatcggcgcc gtaccttgaa ccaggggtga
    31321 cacctgtgaa tcgagccggc cttatctctg tggcaaccat aagcctgttg gccagcgccg
    31381 tccttcctgc aatggcaggg gagcgggctc cgattcggcc tctgttccgg gtgcatgaag
    31441 ccctcgtgca gggtgcgcac agtcttccac tggctcgcgg gaaacacacg atctatccgg
    31501 aacagattcg tggagttatc cgcgacttcg tcaagcgtga acttgccgga cgcgcggtcg
    31561 attgccaggt ggcgctgggc gatccccaac agccgatcgt ggtgccttcc ggcacgatgg
    31621 atctgcaagt cagcgcgaat cattccgagg agccgctcgg ccggcgagtg ttccagattc
    31681 acctgaccgt caacggccgc tttatcaaaa ccgtcgaagc cacagcggat gtcgctgcca
    31741 tcgtcgaagt ggtagtcccg gttcgttcga ttaaggtcga tgaggaaatc gctgcggacg
    31801 acgtggcgac cgaacgtctc gtgctgtacg acctgaagca accgttcgcc atgaatccgt
    31861 cagacgtcgt cggcaaagcg gcgatccgcc cgctgccgcc tcaaaacgcg attcggctga
    31921 cctccctccg tcgcccgttt gcggtgcata agggcgatcg ggtgacgatc gaagcccggc
    31981 aggggggact ctccattcaa accgtaggtg tgacgaagtc gcacggtgaa ctgggtcaaa
    32041 ccatcacggt gtccaatgtt gattccggca aggatctacg agcgaccgtg gtcggtccgg
    32101 gggtggtccg tgtcagcttc taacccatca tccacaaggt atcagacgat cggattcatc
    32161 ctctgcgggc ttgcgctctg cgccctgcca ggctgttcga cttcgtctgc cgcgaagccc
    32221 gcgaaagcgg agttggccaa aatgccgccg cccaagacga ccggctcgct gtggcaggag
    32281 gagaacgggc gggcctatct gtatgaagat ctgcgtgcca tgcgtgccgg ggacatcatc
    32341 acgattctga tctccgagaa acacaaggga tcgaagagcg ccgacaccag cgcggaaaag
    32401 gattcgacgg tgtccaatgg actcggcggc acaggcatgg ggtatctcgg ccttccgggt
    32461 atccgactcg gtggagaagc caagcgggga ttcggcatcg acgccagcgc caaaaataag
    32521 ttcggcggca aaggcgccac caatcgcgaa gacacgttga ccggcaccat ctcagccatt
    32581 gtgaccgaag tgctgcccaa cggcgatttg cgaatcgaag gacggcgcga agtcaccgtg
    32641 aactcggagc ggcagatcat gacgatcgcc ggtatcgtgc gccgtgtcga cgtcaacacc
    32701 aagaacaccg tgcaatcgag cgccattgcc gatgcgaaaa tcgaatattc cggcctggga
    32761 gtggtggacg atgtgcagcg gccgggctgg ttcgtcagga ttctggattg ggtctatccc
    32821 ttctaatgac cgagcagtag gaccgacagg agcgagacag gagtcattat gacgaagcca
    32881 cgcaaacaac aacgggcgat gattatgcag tgttttcgtc tcatgctgtc gagcggcgtc
    32941 cttcggacgc cctcggtgga tccagagctt ctggcccgtc catggacgcc gccgcctccc
    33001 aaaccggctc ccgtctcgga acctcgacag cagatgagtc cctggtttcg ccgcaccgcg
    33061 gtgggcatgc tggcgttcct gacgatctcc ttcttcagcg cttcgctcgc ccatgctgtg
    33121 cgcgtcaaag atgtcgccac gattgaaggc gtacgggaaa accaattgat cggatatgga
    33181 ctggtcgttg gtctggaccg taccggcgat caagtcatcg gcggtcagtt cacgattcag
    33241 gccatgatgt ccatgctgaa caagatgggc atcaatctgg tcattgatcc gattcagttg
    33301 ctcacgcgaa acatcgcgtc agtgatggtc acggccaagc ttccgccgtt cgtcaaaccg
    33361 ggcatgacgc ttgatgcgat cgtatcttcc atggcgaacg ccaagagcct ccaaggcggc
    33421 acgttgctcc tcacgccgtt gaaggcgccg aaccagcaag tgtttgcggt cgcgcagggt
    33481 cctgtgtcga tcggcggatt tttgggaggg accggcggcg cgggcggcgc cacagtcacc
    33541 aagaatcatc aggccgcagg tgtcgtgccg gccggggcga tcgtcgaaaa ggaattggtc
    33601 gtcgatatcg accagtggga taccgtgtcg gttctcctgc ggcatcctga tttcacgacc
    33661 gccattcgca ccgccgaagc catcgacggg acatttggaa aaggaactgc cgtcccggtc
    33721 aatgcaggcc tcgtcaagac cacgctgccg tcgaccttcc aggggagggt ggtggaatat
    33781 atcgcgacga tggaaggatt ggatgtgagt gtggatgtgg cggctaaggt ggtggtcaat
    33841 gaacgcaccg gtacggtggt gctgggtgaa catgtccgcc tctctacctg cgccatctcg
    33901 catggtaatc tcaccatttc cgtgaagaat accctcaacg tctcgcaacc tcctgcgccc
    33961 ttgatcggct ccacccaagg ccagaccaca gtcacgccgg atgtgcagac cgatgtggcc
    34021 gaagagcagt cccggctgat cgtcgtggac cagaccgtta cccttggaga agtcgtgcgg
    34081 gccctcaatg ccgtcggcgt cacaccacgt gacctggtcg cgattctgtc ggcgctgcgt
    34141 gcggccggag cacttcaagc gaatcttgag atcgtgtaag aaaggtctgg accatcatgg
    34201 acatcaagaa tctgttgagc ggccagcttg atctggccca gatgaatgcg actcagcccg
    34261 caggcctcga gcgagtccaa ggcctggaga aggcagggca gaaatccact gaagcgatgc
    34321 ataaggccgg acaggagttc gaagcctact ttatatccta tcttattgga aagatgaggg
    34381 aaacagtgcc aaaaggcctg ttggacagga agggggagca ggtctggtac tccttctacg
    34441 accaggagat tgccaagctg gcgacacagg cgggaggcat tggccttacg gccttcgtgg
    34501 atgcgtacgt cgaaaaaaac aaataaatcg accggttctc ctaaagttcc aggccgacct
    34561 cgccgataga gtactcgact aggggaaagc ctacggaaca ccctgcaaag gggaggagaa
    34621 gcagtcatgg agatctctca caatggccgg gccgcagatc tcgcgaaaat tctcttaggt
    34681 gttcaggaga ctgaccggac acagaacaag aaaaacgcat ctcagaccac gccgtcgcaa
    34741 gaccgcgtac agatttccga gcgggccaag gaactccaac gtctgagggc cgcagcggaa
    34801 caaccggatc acgaacgtga cgccagagtc ggccagatcc agcagtcagt cgaagggggc
    34861 acctataacg tcgacggcaa aaaagttgcg gatgccatca ttcgtaacgt cttgacggac
    34921 gcggtcctgt aacgagtcgc ttcaccggct cgacccttcg ctcgctgtcc tcgtatgccg
    34981 gaacgttact gtcgaggagg aagcacggtg tcgacgctct cgcaaactac tcccactgaa
    35041 tcagacatgc tcgtgcgggg catgctcgac aaaatgctcg cgttccagtc cttgctgctg
    35101 gaggaacagg atgcgatccg atccctgtcc ttcggccaat tcacctccgt caccacgcga
    35161 aaatctcaac ttctagatga gattcgtgat ttcgagcaac ggcggcgtca gcagacctcg
    35221 tctgaattgt ctcgacagga cgcgcgcacc acgatggaag ccaagctgct tgcggcgatc
    35281 ggcgagactg atcgcttgaa tcgattcaat gccgcactca tcgggcagtc gcttgaattt
    35341 ttgcaaggcg ccctgaaacg ctggcaaaga gctccggagt cggcggcgct gtattcgtca
    35401 gccggcacgg cggtcccggc aggcgccggt ctggtgagag ccaaaggata acgagtggtc
    35461 gtatgtcagg actaaacgga ttattcggag tgggttccaa tgcgctggcc agtttccagc
    35521 gcgcgatgtc cgtcaccggg caaaacatcg cgaatgtcag cactcccggc tattcgcgcc
    35581 aggaggccgt cctcaccgag acccttccgg aaaacggacg ccccggccag atcgggaccg
    35641 gcgtgcaggt ctcggaaatc cgccgttccg tcgacagctt tgttgaacag caattgttga
    35701 actcaaatga gcggataggg caattcggcg cctcacagaa ggctctctca caaattcaga
    35761 ttctgtttaa cgatgcgaac gatcaaggca tcgcggcagg gctcaacgaa ttcttcaagg
    35821 cgtggcagga tgtcgcgacc aatcccgccg atctgacggc gcggacggtg ctcctgacca
    35881 aggccgacgg gctgacgaaa gtcctcaacc aggcttcatc gcagctgtcg acccaacgaa
    35941 tctccctcga cgggcaaatc ggaagtgcca tcaatgacat caacgcgctg gcgagtaaga
    36001 ttgcggacct gaatgcccag atcaagttga ccgaagtggg cgggcaacag gcgaatgacc
    36061 tgcgagacca acgtggccgg ttcctgaatg accttgccgg cctggtggat atttcttcaa
    36121 tcgaagatgg aaacgggcag gtcaccgtgt ttgtcggggt cggccaggtc cttgtgacag
    36181 accacacggc cttcaagctc acgggcgttc ccgatgccac caacaacgga ttgctcgacg
    36241 tgcggtatga cggcggaacc ggccccaaca ccgatatcac ctcatcgatc agtggcgggc
    36301 gcctcaaagg tctgctcgat gcgcgggata ccacagcgac cggcctgcaa acctctctgg
    36361 acaccctcac ggcgcaactc gtctcgcaag tgaacaccca gcacaaattg gggtatggct
    36421 tggacgggtc gacgacgcag gacttcttca cggcgacagg aactacggcg ggcacgatca
    36481 gcatggcgct gacggatcga cagaagatcg ccgcgtcctc gactgcggcc ggcgtgccgg
    36541 gcaataacgt caacgccctc gccctggcga atttacagac ggcatcggtc gcgggcttgg
    36601 gaaacacgac gtttcaaagc tactacggtg caatggccgg gagcttcgga gcaaccctgc
    36661 aaggggccac gcgggacctg caagggcagg agattctgca cgatcaactg ttggcccatc
    36721 gggcggaagt gtccggcgtc tcgatggacg aagagttaat caatatgttg aagtatcagc
    36781 gggcgtttca agccgcctcg aagctgatca ccaccagcga cgaaatgctg cagacgatct
    36841 tgtccttgaa acggtaaccg tcattcagtt cacccggagc aggtattatg agagtcgccg
    36901 accaacagat gtacaacacc ctgctcggca atctgcaacg ctcccggtta caaatgctga
    36961 cctctcagga acaggtctcg agccagcgac gggtctcgac tcccgaagac gatccgagct
    37021 cctatggcca gatcgtcctc gacaagtccg cgctgtcgca gaccaagcaa tggattcgta
    37081 atatcgattt cgggacttcg cgagtcaacg ccgccgacca ggcactaggc caggttcaga
    37141 cccttatcac gcgggtacgc gaattgacca ttcaggccag cagcgatacg acctccgccg
    37201 aaggccgtca gtcgatcgcc aaagaagtcc ggcagctcca acggcagctg gtgcaactgg
    37261 gcaatacgga agtggccggg caggcggtat tcggcgggac caaaaccaat gtgactccct
    37321 acgtcattac caccggtgat acggtgacct atcagggcaa caccgaaacg cagtccatct
    37381 cggtgggcga aaaccagacc gtgcaaattc ttgttccggg tagcagcatt tttaccggcg
    37441 gaaccacgaa catgttcgat tcgctccggg atttgctcac ggcgctcgaa agcaacaatc
    37501 gtgcgggtat tcaaacggga atcggcaatc ttgacctggc gacggcacag atcagcgacg
    37561 cgcaggggac ggtcggggcg ttggctaacc gcctgcaggt cacccacgac gcgctggata
    37621 cggcgaccct gacgatttcc aaggccattt ccgacaatca ggatgccgac ctcgctacag
    37681 ccatcagcca gttgagcctc cagcaggttg cggtgcaggc cgccagccag acgttcagta
    37741 agatttttga ctcctcgttg atcaactatc tgcggtaacc aacgctcaag ttcattctct
    37801 cctgtaccga tatacccaat gtacccgtat tcacacgcca aggaaggctt gtatgctggt
    37861 cttaacacga cgtcggggag agggcgtcac catcggtccc gatgtccgca ttgtggtgct
    37921 cggcatcaaa ggcggacagg tcagactggg aatcgaagcg ccgcccaatg tgcaagtgca
    37981 tcgcgatgaa gtgcatgcga ggattcaaga cgagaatcgc attgccgcag gccccggagt
    38041 cattcctctg gaagccttcc gtcaactgtc caataaaaca ggacgccgcg ggagctgaga
    38101 accgtccatg aagtgtcagt cgaccagatt cggcaccttc gaagtcaacg acgacacgtt
    38161 gttggtgttc ccatccggca ttctgggctt tcccgactgg accaaatacg tcatgctcga
    38221 ccatgatacg gatgcgccgt tcaagtggct gcaatgtgtc gaggaaccac agctcgcctt
    38281 cgtcattctc gatcctgcgc tcttcaagcc cgattaccag gtcacgatca cgacggacgc
    38341 cctgatcgaa attcagaagc aggagagcga cgaagtctcc atcgtgacga ttctgacgat
    38401 tccctcggat gatcccacgg cagtgacggc caatctgcgt ggcccgctgg tcatgaacca
    38461 ccgcaccagg ctgtgtaagc agttggtgtt gccggaagat ttgccgaccc gctatccgct
    38521 ctttgccgcc gcttccgcag cgcccagtca gtcaccatcc cagcccctgc aagcctcagc
    38581 tcgctaagcc acattgcagt acgtcacccg caagccgcgt caggcctggt taccttgtgc
    38641 taccaatagc gtttcagacg gcgacgttcg cctggttggg tttcgcgcgc agatgggcaa
    38701 gtgtcactgc gagtcggtgg gattgatctg gcgagataac acggccacca attcctgcac
    38761 ggtgcggtcc agcctcacca gcaggctggg ggcttccgtc aacgttccga gtcgtccgga
    38821 tgcttccagc tgacttgcca acaacacgac atcgggggcg cagagattgc ctgccgtgcc
    38881 cttgagactg tgcgcagccc gctccagcgc cgcggcgtca gcctgcccga tagcggactc
    38941 gatttctttc atcatgacgc tatggcggtc cagaaacagc cggatcaatt gatcgaacag
    39001 ttcggcatcg ccaccgatgt ttgccagcat ggcgccagcg tcgaagactc gcgcgtcggt
    39061 cttgctcagg tcgcgggcag gggcgggggg cgcgacctct gccgcagcct tcaccggcgc
    39121 ccatcgttgc aggatgatgc cgagatcatc cgtcttcacc ggcttggtga catagtcgtc
    39181 catcccggcg gccttgcacc gttcccggtc gccaggcaac gcattcgccg tcaacgcaat
    39241 gatggggatg tgggcgcgcg tccgggacgc gtccggtcgc tcttcctgcg cccggatgag
    39301 cctcgtggct tcgtacccgt ccatcgtcgg catctggcaa tccatcacga tcgccgcata
    39361 ggattcccgc tcgaaggccg tgaccgcttc ctggccattg gagaccacgt caggttgata
    39421 gccaaggcga tccagcatgc gcacggccag tttctgattg accacgttgt cttcggccac
    39481 cagaattcgc ggacgcgggg attgttcggc caatgtgtga cgcgtgatga gcgtcggcga
    39541 gatgggttgc cccttgccgg tcgcagttga tgcggtcgct gccttgtgct ggatcccgaa
    39601 gaccacccgc agacagcctt gtagctgatc atggcggacc ggtttggtca gataggccgt
    39661 gaagccggca cgccgcgccc gctccgcatg gccgggctga atcagcgacg tcagaacaac
    39721 caagcgaact ccggcgccat gctggtgagc gcggatgtcc tgggccagtt gaagcccgtc
    39781 tttgccgggc atcagcatat ccagcacggc cgcatcatag ggttccccgt tcgccgctgc
    39841 ctgctccagc atgtgcatgg cttccgtggc attgcgtgcc tgcccatcgt gcatccccca
    39901 tcccgagacc agatgatgga gaatcgttcg attcgactcg ttgtcgtcga cgatgagaat
    39961 gcggcgaccg ctcagttccg ccgaagggac gatggcaggg gaacacaccg cttgtttctg
    40021 gaagagggcc gtgcaccaga aggtggttcc ttcgccaggc ttgctgcgga gccccactgt
    40081 cccgcccatc aactccacca gttgcttgga gatggccagt cccagtcctg tgccgccgta
    40141 ctttcgtgtc gtggaactgt cggcctgggt aaatgcctga aacagacgcc cttgcacttc
    40201 ttcgctgatc ccgatgccgg agtccgtgac ttcaaatttg atcatcacgc cggccggtgt
    40261 gtcgctctcc aggaaggcct gcagcgttac ctcgccctgt tcagtaaact tgaccgcgtt
    40321 tccaacgaag ttcgtcaaga cttgccgcag gcggcccggg tctccgcgta acgcattggg
    40381 cacggcggca tggaccagcc cggtaatttc gaggcctttc cgctgcgccc gttccgcaaa
    40441 ctgcgacagc acgtcttcca cggtggtccg gagttggaaa tcgagtgtct ccaattccaa
    40501 tttgccggct tcgattttgc tgcattccag cacgtcattg atcagcgaca gttgcgcttc
    40561 gccgcattgc tgaatcgttt gggtaaacga gcgttgctct tcggtcaggg acgtgtccag
    40621 caacaggctg gccataccga tcacgccgtt catgggcgtg cgcaactcat gcgtgatcat
    40681 ggctaaaaac gacgattttg cgcgggcggc cgcttgggcc tgctgcagcg cctggtccag
    40741 ttggcgattg ctctcacgaa gatgatcggc tgtggcgttc agcgcgcgat gcgtcgcatg
    40801 cacctcactg aggtcgattc cgtaggcacg cacgagttga ttgctgggga cggggcataa
    40861 cgtccagcta taacacgcct cgcctctgac tacttcctgt gaggtcagcg atcgcccttc
    40921 cagcaggcag gtggccacaa gcgcgggaag attgtcggga aggatatccg gtttgccgtt
    40981 ggggtcatat ccgaatcggc acagaacctc caccatcgcg gggttcacat acagaatatt
    41041 gccattccgg tcgatttcga caatcgggca ggggctttct tcggccacgg tggcaagacg
    41101 gtcacgatcc tgctccaatt cgcattcccg tgaaatgtcc cggagcgtca gcagtgtgga
    41161 cacagaggga atgccggaaa gacggagttg ctcccattcg atcacggtga gggccggcgc
    41221 atcggtcgca ttcatatcgg tggtctcatc agatgccgcc gcggcgccgc tctggaattt
    41281 ccacaccccc gttgaatgca aggaatcatt cagttcaagg ggttgaccgg taagctccga
    41341 acatcgtttc ccggcgcagt cagccggcga gcggcccagc agacgggccg ccatccggtt
    41401 ggcgaagacg atggtgtgat ccggggccag cagacaaatc cccagtggga ccgcttccca
    41461 gagcaggccc atcatttcag gcgacggcgt aaaccccgaa gggggttgag gatggtgtga
    41521 acgtgaatgc gatggacggc tcatacttat gccccaccta cggcgacatc aagatattgc
    41581 gggaggtcga tctgttcctg cttaggatcc aacgcgcgaa gaatggtgcg caccggctga
    41641 tcggccgcct ggttttgaag atcactgtcg atggggggat tctgaatttc cccatcgggc
    41701 cctctgatga tgcggatacg cggacgcaga cggtcctcat gattgaggcc gatgacgacc
    41761 gcgcattcgt tcgtattgag cttgatcagg cttccgaggg gatagacgcc cagcatcttg
    41821 atggcgactt cgacgagatt cttatcgtat cggcctttcg cgcccaacac aaacagttga
    41881 cggatggcat cgtgaggcaa cagcggtgcg cggccatagc gcgaggtcac gagatcgtca
    41941 taggtatccg ccagggccat caactgcgcc agcggcgaga gtccgcccga tttgaccaac
    42001 ttggggaatc cgctgccgtc ctgaaactca tggtgttgcg cgatgatttg ggagaccgtt
    42061 tcggggagcg agtcgatttt cgataacacc gtggcggcca actgcgggtg ttgccgcatc
    42121 aggacttgtt cctgctccgt caaggccgag gtcttccggt acaagttgcg gggcagccga
    42181 acgaagccga tatcgtgcag cagcgcgccg aggccgagcg actccatgtc gctttccgtg
    42241 cagctatttt ccacggccag aatgagtgtc aacacacaga catccatgcc atgattggcc
    42301 agcgtgccgt cgaagcgacg catcttatcc agacaaaact ggatcatcat cgcttccggt
    42361 tgttcgatga tctgcttcaa caatttgctc actaccggtt tgagcgccgc aatcttcggt
    42421 ggctggccgg cttcaaggtc ggtgaacacg cgttcaaccg cttccatcgc ttcccggtat
    42481 gtcgcggccg ctgccgcggc atgcgaagaa tggccgttcg tcgacgcatc gacggattct
    42541 gcagaagcgg gcgacggctc aggaaccgga gcagtggatt ccggcgcggc cactggagct
    42601 gcgcctacat ccaacccgcg atccgtatca atggtgacaa tctgaatgcc gtgccgtttg
    42661 agttgcacga tgtcgtcggg gttggatacc agccacttgt gaaggagaaa gggcgtcctg
    42721 taccagggtt gatccagacc tgcgatgaac atgccgacgg tgagctgatc gatagggata
    42781 cgttttgtcg atgccatgtc agagttatgc ccaatcctca aaaggcctgt gctgagtgcg
    42841 cccagggtgt ggtttctgtc ttgtatctat cggtcgatgt gagaacggtc tttactgaga
    42901 aatatgtcgc gagcgtgaca caggcttcag attctgaagg catctgccga tagggctgac
    42961 aggggtgtgt ctatgccttg catgactcgc gcggggagac ttcatgagtg atctcatcgt
    43021 ccatcgtcat ccagcttcat tcctcgaagt ggggctctcg ctgcaactca gccttccggc
    43081 ggagggcacc ggagcccagt acggcgccac ggtgctggga tggaaagagc gcagctggat
    43141 cgtctgtgaa tggccgtttc atttcggcca accgatttct tgcgctgcgg gaactccctg
    43201 tctgttgcga tacatctata atggaaaaat gatcggatat cgtacggacg tcctccatgc
    43261 gcagttgcag ccgtttccat ttttactgct ggcttttccc gccacgctgg acgaagtgcc
    43321 gctccgtaaa catggacgcg tgacggcgca ggaacccatc gttctgttac aagcggatca
    43381 gaacctgcgc aatcagccaa ccggtagtcc atgccgcatc ggcggattgt taacagacct
    43441 cagcgcgtcc ggatgcgcgg tactggtgca gcggccgatg caggactttt tccccggcat
    43501 ggtcatacgc gcagaattcg aaatcgtggg gacgggccac gtcaacaatc tgacaggggt
    43561 cgtccgaaac gtgtcaatgc aggcagacgg aacgcatctc ggcctggaat tccggttcga
    43621 cgggaaggaa accatcgagt accgggggtg gggaggatcg gtcagaaaag cgctggaatc
    43681 gttcgtcctt cagaaacatt ccttcgagtc cgcctagact acagcggttg ccggggactg
    43741 ggaatttttt gcccggttac aaagcccgca gcagttctcc aactaagaaa tcctacggtt
    43801 tcccatcctc cctctgccat cctcagtggc atacatcttg attagtatcc tggtgcggtg
    43861 cctggtctgt tgacagacgc aggcctcctt tttggcgcga gagggactac taccatgacg
    43921 cttcacgcac acagtgccaa gctcaacccg cgatggtgcc tggctcagga tacgatcgac
    43981 cggcaggata ttgatcattt gatcgactgg ctgcggacct atccgcgact caccaagggc
    44041 gcggtgaccg tcgatttcga gcaacgctgg tcgcagtggc tgggacgccc ctattcggtg
    44101 cattgcaatt caggatcatc ggccaatctc ctcatgtatt acgccttgct gcgatcggga
    44161 aagttgcgca ataccaatgt catcgtcccg agcgtcggat gggtcacctc catcgctccg
    44221 gccatccaat tcggatttac tccgttcatg tgcgaggccg atcccgacac attcggcctc
    44281 gatctcaatc atcttgaaga cctcctgaag cgtcatcacg cgcagacggt cctgctggtt
    44341 caggtccttg gtgtacccca ccggatgcgt gagctgcaga cgttgaaaga tcgatatggc
    44401 ttttatctcc ttgaagatgc ctgcgccgcc atcggagccg aatacgacgg caaaaaggtc
    44461 gggacattcg gcgacatggc gagcttttcg ttttacttcg ggcatcaaat gtccacgatc
    44521 gaaggcggga tggtctcatc cagcgacaag gagctcacga atcttctcct catgttgcgc
    44581 agccacgggt ggagcaaaga tgtagaccag gcgcgccacc aggaattggt ggaccagtat
    44641 caggtggacg actttcattc gcccttcgtg ttctacgaac cgggattcaa tctgcgatcc
    44701 accgatctga acgcattcat cggcctcgag caattgcgta agctggattg gatgaccggc
    44761 cgacggcaag ccaatcacga tcgctatctc gagcagctgg ggacgcggtt ttacacgcag
    44821 cggccgccga aaggcagcaa agtcgcaagc atttctttcg gcttactggc cgagagcccg
    44881 gaacaacgga aacgcatcgt ccgggcgttg gtggccgagg gagtcgagac acgcatcttc
    44941 tcagccggca atctgggtct gcatccgttc tggatgaatc gctacgggaa ggccagtttc
    45001 cctgtggcag accgagtcca tcattgcgga ttttttctgc cgaaccatgc ctcaatgacc
    45061 gaccaggatg tcctgcacat ttcccgtatc gtcctggagg ctgcatgagc aggggagcag
    45121 ggcagcacga gtggcacagc cagaaggagc gcgtgtaact atgacgacag gctcgacaca
    45181 tgtcctcatc accgggggcg ccggatatct gggttcagtg ctgaccagac gattgcttga
    45241 ccgggggtat gcggtcaccg ttctcgacaa ttttatgtac cggcaaaaca gcttgatgga
    45301 ttgctgtgcc gaggagaaat ttcaagtcgt gcgcggcgat tgccgggatg agcggctggt
    45361 gacagacctg ctgcgcaaag ccgatatcat cattccgctt gccgccctgg tcggggctcc
    45421 cttgtgcgac agagaccgtg tcggggccta cacggtgaac ttcgaagccg tgcaactgct
    45481 ctgcaaactg tcctctccgc ggcagcagat cattttccct gtgaccaaca gcgggtatgg
    45541 catcgggcaa cctggtgtgc cctgcaccga agactcgccg cttcgtccca tcagtctcta
    45601 tggcgaaacg aaggtgaagg cggaacgcgt cgtcctggac cgtggcaatg cgattacgct
    45661 gcgcctggcg acggtgttcg gcgtgtcccc ccggatgcgc atggacctgc tggtcaacga
    45721 tttcgtctgg cgggcggtgc atgatcgagc ggtggtggtg ttcgaaggcc actgcaaacg
    45781 caattacatc catatccggg atgtcgtgcg cacctttctc catgccatcg accacttcga
    45841 cgatatgaag aacaggccgt acaacgtcgg gttggacgac gccaatctct cgaaactgga
    45901 actctgccga gaaatccagc gagtccttcc acagttcgtg tctttcgaag ccccgatcgg
    45961 ggaggatccg gataagcgcg attacatcgt ctcgaaccag cgactgctgg agaccgggtt
    46021 caagccggaa tggtccttgg agcgcgggat ccgtgagttg atcaagtgct acacggtcgt
    46081 caaagccggt caatatgcca atgtctgagc gcagcgcaag ccggcaggga gggcgaggat
    46141 gatcatcagc cgaacgccgt tccgtatctc gtttttcggt ggcggtaccg attaccccgt
    46201 ctggttccgg gaacatggcg gagcggtgct ggcaacgacc atcgataagt attgctatat
    46261 cagctgccgt cagttgccgc cgttcttcga gcatcgcacc agaattgcgt attcgcgcat
    46321 cgaactggtg aacaacaatc aggagatcga gcatcccgcg gttcgcggcg tgctgaagta
    46381 cctcaatatc gacgacgggc tggagattca tcacgacggc gatctgccgg cgcgaacggg
    46441 cttgggatcc agttcctcct ttaccgtggg cttactccac acgctgtacg ccttgcagca
    46501 catcatgccc agcaaagcgc aactggctca ggctgcgatc catgtcgagc aggatgtgct
    46561 cggcgaggcc gtgggatgcc aggaccaggt gcttgcggca catggcggct tgtgcaaggc
    46621 cacgtttttt cagaacggtg acatcgggca tgcgccgatc attatgcgtc cggagcggct
    46681 tgcggcgttt caaaaccatc tccaattgta cttcaccggc ttttcacgca ttgcatcgga
    46741 agtggctcgc gagcagatcg accgcaccaa ccaacgccgg agtgaattat cgacgatgct
    46801 gcagatggtc gatgaaggtg tatcgattct taccggagac cgcgatctgg cagacttcgg
    46861 cagcatgctg cacgaggcct ggatgctcaa acggcgactg acctcgcgca tcacgacgcc
    46921 ggctatcgac gcgatttatg cggcggccag agaagcaggc gcgctcggag gaaaactcct
    46981 gggtgccggc gggggcggat tcatgctgct cttcgccagg ccggaggatc atgagcgaat
    47041 tcgtctggcg cttcccggat tgctgcaggt gccgtttaag tttgagggcc tgggcactca
    47101 aatcgtgttt taccaggaag atcagctgat gctggatgac gtgtgggcgc aaagctccgc
    47161 gcagaccgca gcccggctga aggccacact catcggaaag gcggcttgat ggctcagttt
    47221 gccgattgtg atgtgatcat tctctgcggg ggattcggaa cgcgactgca atccgttgtc
    47281 tcggatcgtc cgaaaccgat ggcggagatt cacggtcgac cgttcgtgtc attactggtc
    47341 gaacatttcc tgcgtcacgg cgcgcgccgc tttattttca gcacgggaca tttcggcgag
    47401 atgatcgagg actggttcct gcgccaccgc ggggcctatg aaacgctgtt cgtgcgtgat
    47461 cctgtcccct tgggcaccgg tggggcactg gcgcatgcca tgccattggt gcgcagcaac
    47521 ccgtttcttg tgctgaacgg cgactcgttc tgcgagatcg atcctgagcg gctgttgcga
    47581 ttccatacgc gcaagcgggc tggcgcgacg attgccgtca cccacgatga gagccgcgaa
    47641 gacaccggcg ccgtggcgtt gggagaaggt gaccgcgtct tatcgatggt ggaaaaaccg
    47701 cgaacgcgga cagcgaacta tcataatgcc ggcgtctacc tgttcgaccg cgccgtgacc
    47761 acactctttc cggacatgcc ggtctggtct ctggagcggg acctgctgcc gcgtctcgtc
    47821 acgcatcagt gttacggatt cgtcaccgcc agtccgttgc acgacatcgg gacgcccgaa
    47881 cgtctggccc atttccgtga aatctggaag gacacctcgg cctatttccc aacctcgctt
    47941 cttcatcaga cacaaggatc gatcgcacga tgacccagca gagagcgtcg atgccgcagt
    48001 tcaagacccg accggctttc tcttcacgcg gcgaacgttt gatcggacag gaaatgtttc
    48061 gtgttttgga tcaggcgcag gcgctggaac gccagggcca ccgcatttat caccttgaac
    48121 tcggcaatcc gcgcgtggct cctccgtctc aaattcttga ggccacgatc ggggaactgc
    48181 aggccaagcg cgtcggatat gcgccgatgg gcggcttaca agaattgcgc tcggcagttg
    48241 ttgaacgcta cgcctcgttg agcgggaaac cgattgccgc ttcgcaggtt gtcatcagcc
    48301 cggcgaatat gctcattcac cagttcctgg atatcacctg caatcccggc gagcggatag
    48361 tgctcttcac gccggcgttt ccctcctatt gggccgcagc cgcacatctc ggacttgatg
    48421 tcgtacccgt gccactcccg gcacgcgacg gcttttcgct gtcacggccg tccatcgatg
    48481 cggctctggc ggcaaagcct agggcgatca tcgtcaacag tgccaataat cccaccggcg
    48541 ccgtctatga gccggcgatc ctgcacgagc tggtggcacg gtgcggccag gcaggaatct
    48601 ggctcctcag cgatgaaacc tatgccgatc tgaccttcgc cgggtcgcat gtcaccctcg
    48661 cggctccgca ggccggacat gtcgtcgcga tgtcgtcgtt ctccaaggtg ttgtccgttc
    48721 cgggatttcg caccgggtat gcgctggcgc acgaggcggt ggtcgccaaa atcgctctgt
    48781 ccaattccac cctctactcg tgtctgcccg gttttaccca gagcggctgt ctcgcagggt
    48841 tgtctgtgct tgacgcctat gctgccgagg tgcgcgcgcg taatcggcgc ctcattgcaa
    48901 cctgtcatga ccgcatcact cacgacggtg tgttgcagtg ccgccgtcct gatgcggcgt
    48961 tttatctctt tgtcgacatc agcaggaccg ggctcgacga tatgacgttt tgccggcgcc
    49021 tgcttgagga agaacatacg gcggtgacgc cgggtcgctg cttcggcgat gcctacgcgt
    49081 cctgtatccg acttgcggtg tgcggacagg aagaagatgt gctggagggg gtgtcgcgca
    49141 ccatcgcgtt tgccaagaaa ctgggagggt gccatgtcca agccgcttga cgtcttgctg
    49201 gtgacaccac ccagccgcgt ccaggtctac caggaactca gccgggactt cgccgccatc
    49261 gagccgccgg tctggtcggg cttgatcgcc acctacctcc ggcaacacag ttgctccgtg
    49321 gccattcttg atgcggaggc tcaagggctg acccatcagc agacagccga gcagatcgcg
    49381 gccatcgccc ccaggctggc ggtcttcgtc atctacgggc aacaaccgtc ggcgtccacg
    49441 cagtgcatgc ccgccggccg gaaagtctgc gagatcctga atacgctggc cgatattccc
    49501 acattggtga tgggcaccca tccatcggcg ctgcccaaac gcaccttgtt ggaggaaccc
    49561 tatacgtatg tctgtcaagg ggagggaccg gcgacaattc tcggcctcgt cgtcgccttg
    49621 cgcgccccgc agcattcact ccgcgagatc ccgggactgt ggcatttgga ggaggggcat
    49681 gcggtcggca atgtgcccgc gccgctcttc tcgaatctcg accgcgacct gccggggcaa
    49741 gcgtgggatc tgctcgatat gagccgctat cgcgcccaca attggcactg tttcgggaac
    49801 ccggatgcca gaactcctta cgcctcgctt cagacgagcc tgggatgccc gtttacctgt
    49861 tccttctgct gcatcaacgc acccttcacc accccgatgt tgcgtgtctg gagtcccgac
    49921 aatgtcatcg cccagatcga ccgtctcgtg cgcgagtacg gcgtctcgaa tatcaagatt
    49981 cccgatgaaa tgttcgtctt gaaccgccgc catgtcatgg gtatctgcga ccgcatcatc
    50041 gaacggggct atcgcctgaa catctgggca tacgcgcggg tcgatacggt gcaggacgaa
    50101 gtgctggaga agctggcgcg cgccggcttt acctggctgg ggctcggcat cgaatccggc
    50161 agccagcatg tccgtgacgg ggtcgagaag ggccggttcg gtgaacgtga gatcgtcgcc
    50221 acggtggagc gcatccgcgc tcacggcatc catgtcgcgg caaattatat tttcgggctt
    50281 ccggacgaca ccctcgagag tatgcgcgcc acgttggatt tagcgctgac cctcaacact
    50341 gaatgggcga atttttactg tgccatggcc tatcccggct cggcgctcta cggtcttgcc
    50401 aaacaacacc attggacgtt gccggatgat cagggcggcc ccggctggat cgggtattcg
    50461 caacatgcgt acgaatgcct cccgctgccg actgacagcc tgaccgccac ccaggtcctg
    50521 gattttcgcg accgcgcctt tcaggagtac ttcggccatc ccagctatct ggccatgttg
    50581 caacgaacgt tcggctcgca cgtcgtcgct cacgtagcgg agatgtgtcg gcatcaggtg
    50641 cgccgtcgtc atcatgatca ggcgtcgcat caggcggcgt agatgagcgc agggaaagga
    50701 gcatccatga tcaaggtggc agacttcatc atcaatacgt tggcggaacg gggcatcgac
    50761 aagatgttcg tcgtgtacgg cgcggccaac ggcgatttga tcgatgcgtt cacacggacc
    50821 gccgcgaccc aatatgtcgc cgtcatgcat gaacaggccg gcgggttcgc ggccgaagcc
    50881 tatgccaaag tgaagggcat ccccggggtt gccatcgcga ccagcgggcc gggcgggatg
    50941 aatctgctca cctcgatggg caattgcttc tacgattcga tcccctgtgt cttcctcacc
    51001 ggtcagatca actcgcggtt tctgcgcccc gatccctcaa tccggcaaat cgggtttcag
    51061 gaaacggaca ttgtcgcgat ggcgtcgccg gtcaccaaat acgccaagat gatcctgaat
    51121 cctgaagacg tacggttcga gctcgaaaag gccctgtgga tgtgtcaaga gggccgaccg
    51181 ggaccggtgc tgctcgatat acccctcaac gtgcaaaaag cgatggtcga cgccaaacag
    51241 ctcacgggct tcgagccgcc cgcagccatg agcttcgacc tcacggttgt cgatcagcag
    51301 atcgcccgtt tcatcagcga cctccagcag gcggagcgcc cggtgattct ggtgggaggc
    51361 ggcgtgcgcc tggccgatgc acaggacgat gtacgggcct tggggcgccg gttgaacgta
    51421 ccctgttttc cgacctggaa cgcgctggat gtgatcagtt ccgattatga gaactacggc
    51481 ggacgggtag ggacctatgg tggcgccggc cgcaacttcg gcattcagaa cagtgatctg
    51541 ctcctgtcca tcggcagtcg catttctgga cgcatcaccg gcggcaacgt tcagagtttt
    51601 gctcgacagg ccaagaagta tctggtcgaa atcgatccgg ccatggtcca gcggaaattt
    51661 caacaggtgc cgttcgatgt caacatcctg tgcgacgcca aggcgtttac acagcgtgtc
    51721 ttgatcgcgc tcgatcgacg cagcaaaccc gtgccggatt tctcagcctg gacgggccgc
    51781 gtcatggaat ggaagcggcg gtacgatccg gttcgtccgg agttcttcgc ccaacgtgag
    51841 cgcgtccacc cctatgcgtt catgcgccgt ctctcagaaa agatgggagc cacggacgtg
    51901 ctggtgggcg actgcggcgg caatatcgtc gtcagcaacc atgccttcga gaccaagtac
    51961 gggcagcgca acctgaccaa caatggcaat tctcccatgg ggttctcctt tgcgggagca
    52021 atgggcgcct ggtttgccga tccaacgaga caagtggtct gcacgatcgg cgacggcggc
    52081 ttcaacatga acccgcagga gttgcagaca ttcgtcaact atggcgtcaa agtcaaaacg
    52141 ttcattctga acaaccatat ttacggcatt accaaggcct accaggaaac caatttccag
    52201 ggacgtgccg aagcctgcgg gccgaagggc taccatccgc cggatttcct caaggtcgtc
    52261 caagcctatg gcatcaagac cgtcgccatc gcgaaccatg ccgagatgga tgccaagatc
    52321 gacgaggtgc tgcagtttga cggaccggtc gtctgcgatg tggatatgca cgagttccat
    52381 acgtacgaac cgagaatctt cggttggaag acgcccatcg aggatatgta tccctatctg
    52441 cctcgagaag aattccgcgc caacatggtc atcgaaccgg cggaaggatg gatgagtccc
    52501 gagtatcccg atgtggtgcg ccggacggat cagtcacagc cgtagtgagt ggagcgacac
    52561 gatgcggcgg cccggttagt gaggcaaggg agctcatcga ttacatgagg tgatgtatga
    52621 acgtcttgtt actgtatccc acatggaccg gtgcctacgg actgttcggc cattttgccc
    52681 ggcggaattc cacctggccg ccgctgaacc tcgcgctcct ggcggccatt gctgaacgcc
    52741 acgggcacaa tgtcacgatt ctcgacggcg aatccgagca aattcccttg gaggagatgg
    52801 tgcagcgggc ggtggcgatg aagcccgatc ttatcggctt taccgcgacg agcccctttt
    52861 tccatttgag caaaaccgta gcggaaggga tcaaacgcct ggctcccgag attcccatcg
    52921 ccgtgggagg ccctcatatc accatcatga aagaacaggc gttgttaggc tgcttcgact
    52981 atgccttcat cggcgaggcc gaggagtctt ggcctcaatt cctgaacgcg tatgaacaac
    53041 gcaaggacct gtcgacggtg cgcgggatca tatatcggcg gaatggggaa ctcgtctcga
    53101 ccggtcagcc ggctgacatc acggatctcg acgccttgcc gattcctgcg cggcatcgat
    53161 taccgatgtc ccggtacaag ctcggcacgc tccgcggacg tctcccgttc acttcgattc
    53221 aaaccatgcg cggctgcccg tggaaatgta ttttctgcgc ttcggaagcg ttgaagacca
    53281 ccgagatgcg tgtgcgctct ccgcgatcgg tcgtcaacga gatgaagcaa gtcgtggaga
    53341 tcttcggcac gcggcacttc tatattgtcg acgatgtgat gacgctctgg aaagaccaca
    53401 ttctacagat ttgtgacctc atcgatcagg aagggttgca aattaccttc gaaggcagca
    53461 ccagggccaa tctcgtggaa gaagacgtga tcgcccggct ggtcaagagc gggctcatcc
    53521 ggctgtcgtt cggactcgaa accgtcgacc ccgaaatacg gcggacgatg aaaaaacagg
    53581 tcccgctgga gcactacgtg aaagcaaacg gcatctgcaa ccggtacgga gtggaagcct
    53641 tgaactccgt gatgatcgga ttgccggggg aaacacgaga aaccgtgcga gcgacgctga
    53701 actttttacg gcaggccaga gaagtcaagc aagcgaattt tgccatcgcc gtgccgtatc
    53761 cggggaccga gtttcacaag ctggcggtca acggcgaaaa aggcgttcgg ctgatgacgc
    53821 aggatttctc cgaatatcga cgctatggat cggccgtcac cacggtgggg gagctgacgc
    53881 ctcatgacct catcgatctg cagaatgagg gattcgtcag tatttattcc gcgccctggc
    53941 gctggattcc catgctgcag aaacacggca tgatcggcgg ctcactcatg ctggtccgtg
    54001 tggcacgcct cctgtcaaaa aaactgtttg ccggccacgg gcaatcctca caggagcagc
    54061 tcatcagcct gggagatgga gcgttcggtc gcatccagga atcagccatg agctcacagc
    54121 cggttggagt cgctccaacg ggaccgaaca gcagcgctcc attgttgacg atcgcctcgt
    54181 catcccgggc gggagccgcg cccgccgggc atttcggtca tcccacaaac cccaatggct
    54241 aatggtccca acggaaaggc ctcatgacac cacgtccacc catgaaagtg cttctcttgc
    54301 agctggagtt tcccacgtgg aaacaagctc gcgcttggac gtatccggcc tgttttggag
    54361 tcgctgaagg gttacgggca agtggcgtgg actgtaccac gcttcccctc atcgccgacc
    54421 cgccctattc cagtacggcc tggctggcgc acatgaagcg gatgctcaaa gggcaacggt
    54481 tcgatcaggt gtgggtgtgg ctggttcatg cgcccctcga tgaggcgatt ctgaactggt
    54541 tgagtgagtt agccccgatt cgtgtcggca tcatcatgga atccttgcag tacagcgagg
    54601 aggactacgc atgggcgcct catcttcggg cacggcaggg gttcgtggaa caacaggtcc
    54661 gtgcattgac ccatgtgctc gttccggatg aatgcgacgt agagcgaacc gccgcacgca
    54721 ccggcgttcc tgctctctgg tttccgccga tggtgccgga acgctttatc agtacgccac
    54781 agaacgcacc cgttcatcga gtcggcgtct tccacggcgt gccctacggt ccacgcgaac
    54841 agtggatcca tcatccggcg ctgaaatcat gcctcacatt cgctcgccca tcggctccca
    54901 cgttgtttca acaattattc gaccggctgc agcagtcagc cattcagcgg ttggccacac
    54961 cggcgggaat gcccgccgct gagctcactc actacacgac gatgttgcag gaagtgcgcg
    55021 aaggggaatt ccgggagtgg atgactcaat tgccgcaatg ggcggcgatt gtcaatctac
    55081 cgagtctggc gaaatttttt ggtggtcggg tgtttgaagg gatcgccgcc ggccgaccgg
    55141 tcgtgtcgta cgccgtgccg ggacatccga tgaacaacag cttgttcgtc gaaggggagg
    55201 agatcctcta tttttcaccg agccggcccg actcactggc ggaggttctg gaccgtctat
    55261 tggccgatga cacattcgcg acgcgaatag caggcaacgc ccaacacaaa ttgcgcacct
    55321 atcacaccag cgagcgacgg cttgtagaga ctctcgcctg gatacagtcg ggaaccacgc
    55381 ctgtgtatgg tgaacgagag gctggcgccg cctacatgct gtcctctgcc aagaccggcc
    55441 acgcggctcc atctgtcgaa ccgcgcccca cacaagccat tggccacgtc gatacgacga
    55501 tcttcatcct cacggttgac gatcccgcgt atcccgcgtg caaagcagcc gtcgatgcgc
    55561 agcagggcgg tccgtttcac gtcgagatca tccgcaatgt cgcccccttc agcgcggcgg
    55621 ctcaacgcat gatcgctgat tgtcgaaccg agttcttcat ccaggtcgac gaggacatga
    55681 tcctcaacct cgacgcggcg accaagatga cggacctcat gcgccgggcc ccgtcggagg
    55741 tcggcatgat ctgttttcat ctctacgatg aagatcgtgc ctgccggatc cagggcatca
    55801 agatctatcg cacggcggcc atgaagccct ttgccttcca gaatgtgaag gctagtgaga
    55861 tggatctgct cgagcagatg ggccaacggg gtgtccggtg ggtgctgcat ccggatgtcc
    55921 tgggtcgcca cgggacggtc tatacgccgg aaaccatcta tcgccgctac aagacgatgt
    55981 atgaaaaaga catccggcag tggaataccc tgacctcgga catccgtagg aaagccgatc
    56041 agtttcgcgc gacaggggac ctgcttgcac tgtttgcgct gctcggggcc gcacatggaa
    56101 tcatcgaagg ccctcgatcg gccgaccgtg aaaaagatgc gcgagcgtat ggattgaacg
    56161 aactggatat ctttgcccgg ctcttccggc agaccccgcc ggccagtcaa ccctatgatg
    56221 caacgcgaag cggaacgccc gtcgctaata gtccgttggc gccggaggag gtgcgctggg
    56281 gaggggaggc agcgatcggg aatgcgcccg accagcaggc cttccagcgc gcgaccccgg
    56341 cagcgcagcc acctcgtcat catgccgccc gaatcctcat tgtcaccccg tatttttggc
    56401 cctccgttgg cggcgtggaa aaggtggccg aaagtttagc cgtcggtttg cagtctcgag
    56461 gacatgccgt ggaagtggcg acctatgcgc tcacggagcg gtcggcacaa tcgcatcgtg
    56521 gcatccccat catcgatctg acattcccgg ccgactttga aacgaccagt ccctattcca
    56581 tgctggaagt gaagcggctg atcgagtcag ggcactatgg ctgctgtatt ctcctgggcg
    56641 ccccgctgca tctgatgttt tatgccgcac tcgccattcc gaaggaccga ccggttcggt
    56701 ttatccttca gccgaccatc aacaaagaga tcgctgaatc attacagggg agcgagcaag
    56761 ggcgcaccat gttgtctcaa ctgggacaac gcgtgaacgc agtcgtcacg ttcggcggca
    56821 atggatacga cgaacagttc tttcaagaac gccggattcc aacctgtgtc attccgaacg
    56881 gcaccccggc actggcgccc aagaatgatt tcaggcagcg ctacgggatt gcccgcgaca
    56941 ccttcctggt gattcacctc gccaatctct acaaggttaa aaatcatccg ggcttgctcg
    57001 atgcgctgga tcacctgccg aatggcgtcc aactcgtgtt gatcgggaat gacacacacg
    57061 aaaccgaata tgtcgagacc gtgaaagccc gtctgaagac tcgaccggat gtccgactgc
    57121 ttgcgggact ggagcctgcc gatgtcgcct cagccctggc ggcagccgat ctcgtggtgt
    57181 tggcctcgca tgcggaagtc tcgcctctct gcctactgga agcgatgagt gtcggtcggc
    57241 cctggctcgc gacgccggac tgcggaaccg cagccgagca tgcggggggc ctggtgcttc
    57301 ccctgtccca gttcgcctcc accgtaggat tgctgaagaa acatccggag ttgtgcgccg
    57361 agctcggacg actgggccgt gaacattggg cacagtcgca tgactgggcg accgtcctga
    57421 cggcttggga ggacctgatc gaagggcgtc cggtgtcaca ttcatttggc acaccagtct
    57481 ccatcgccga acgacgagag actctccgcg aagcattccg tcgtcttctg gcacaggaag
    57541 gcactgccgt agcgtcgact gcgtccacgc tgcaggtcga aacgaaaggg gtcgtgatgg
    57601 acgggttaaa ccagggaccg cgaggctcgg tagaccgact cgccatatcg aattccgccc
    57661 cgacgcctca agaacagaaa cagcaggacc aattttacat cgacatgttc gtgaagagcc
    57721 gtggctggtc atcgccaggc cccaatcatg acgaagcggc tcgttggagc aaaatcgccg
    57781 cctttctgga atatctgctc cgtcgcgtgc gccagacaga ccctcagcgg caacttcgca
    57841 tgttggatgt cggatgtgga cgcggctggc tgacgaatct ggcgacggcc tatggcacat
    57901 gcgaaggcat cgaaccggta gcaggcgtga tcgagcatgc acgacggcta tttccgcacc
    57961 tgcgattcga agcaggtact gcccagagcg tgctcagtcg accggacttt gctccgtatg
    58021 atgtcatcct ctgttctgaa gtcatcgaac atattccaca cggccggaaa gaagggtttg
    58081 tcgcagaatt gagcatgctt ctgaagccgg atggctatgt cattctgacg acaccgcgcg
    58141 gggagatgtg ggaacagtgg aaaaccatcg cgccgccttg tcagccggtc gaggattggg
    58201 tcacggaagc gcaactgcgc gacatcttcg taagccaagg gttctgcgaa ctcggcctcg
    58261 aacgggtgca ctgcgaactt ccttcgctgc gatatatccc tgcgcccacc ccggcggatt
    58321 tccataaact gaacctggtt cccatttatc aaatctgggc ctgtcagcgt cggggtgcct
    58381 ctcccgttcc ctcgtttaca cgcccgccga tggtctcggt cattgtgccg acctacaatc
    58441 gaccagatcg tctgcaggtg gcgctctcca gcatcctggc gcagacctat caggattttg
    58501 agatcatcgt cgtcaacgac ggcacggtcg atgtgagcga cgtgatcgcc cccctcaacc
    58561 gggacggtcg tatcaccgtg atccggcacg atcgcaaccg tggactggcg gcggctcgca
    58621 atacgggaat ccgggcagcc aaaggcacct acatcgcgta tctcgacgac gatgacacct
    58681 atctcccgga ccatctgcag acgctggtca cgacattaca gggcggcgag cacaaggtcg
    58741 cgtacacgga cgcctggcgg atccatgaag tacggcaggg tgatcagtac gtggtgaccg
    58801 gccaggatat cccgtattcg cgcgacttca actgcatcga tctcctgttg tgcaactatt
    58861 tcccggtgct ctgcgtcatg catgagaaag cctgcctcga acaggtcggc gtcttcgacg
    58921 aatcgctctt cgcgcacgaa gactgggatc tgtggatccg gatggcgacg atctacccgt
    58981 ttctccacat caagcacacc accgcggcat tcacctggcg tcgcgatggc tcatcgatga
    59041 ccagcagcac cagcgacacc taccggcgca cgaccgaaat catctatcga aaataccgac
    59101 cctatgccga gcgcatagcg ggggtgctgg aggcgcaaca aaagaaattg gaaggcatgc
    59161 gatccgacgc ccaggccaag acgttcgatt gctccattat cattcccgtg tggaacaacc
    59221 tggccctcac cacccagtgt ttgacagccc tggcggaggt cacacagggc gtgtcttacg
    59281 aagtcatcgt ggtggacaat cactcgacgg acgacacccc agcgttcctg gccggtcttg
    59341 gcggcgatgt caaaatcatc accaacaacg acaatctggg atttgccaaa gcctgcaacc
    59401 agggggctca ggcggccaag ggtgaatacc tcgtgttcct gaacaacgac accattccac
    59461 aagccgggtg gctcagtgct ctggtagaag aggtaaaagc ccattccgag gtggcggtgg
    59521 tgggaagcaa actgttgtac gaagacggca cgattcaaca tgcgggagtc gccttctcgc
    59581 gtgagttcct gatgccctac catatgtatc cgggagtgcc ggcagacgca ccctttgtct
    59641 cgcgccgccg cgagctgcaa tgcgtgaccg ccgcctgtat gctcatccgc cggcaggtat
    59701 tcgcgcaggt cgggggattc gacgagggct ataagaacgg atttgaagac gtcgatctct
    59761 gtctcaaggt cggcgagaag aattggaaaa tcgtctacca gcctcagagc acggtcattc
    59821 atttggaaag ccgcacacct ggacggaagg cgcacgagga agaaaacacc cttcgttttc
    59881 gggagcgctg gggccgttgc tggtgggtga cggacgagga tcggctgcat tttgacgatg
    59941 gctattctgt gcatacccat ctcaaagatg gcctccttgg gtacacgctg tacctcatca
    60001 ccgatcctgc gattcgagca gaacgggcat tcgtcgccga cgtgcagcga gcgacagccg
    60061 tgaaggatca gcagaagatt gtctccttgc tcaaacgaat cgaacagtgg cccaccgatg
    60121 catggattct tcgttggggc gccttactaa gcgagggcat cggccatctg gaattggcgc
    60181 tttcgttctg gaaacgtgtg atcagtctga aaaatgaccc gcaggctcgc ttggcgttag
    60241 cgaagcaggc actggaatca ggcgccatga aagaagcgga tcatcatgtg accgccttgc
    60301 tgcaggcaga accgtcacac ggggaaggct ggctcctgcg cggcattctg gctatgcagc
    60361 tcaatgccta ccgagacgcg gaacaagcct tcgagcaagc gaaacaggca ggcgcacatc
    60421 cgcggaaggc catgctcggc ctggtcatgg cagccatggg ggacaatcgg cctgaggccg
    60481 cctggtctca cgtggcaggt ctttgcgccg attaccccga tgatgaagaa tgcatgcact
    60541 ggatgctgaa atgcggaacc ctccttcaac gctgggacgc cctcgcgaca cggctttcct
    60601 cgtttgtcgc tcgcaatccg ggcaatattg ctatgcgatt tgccctcgcg ggagtactgt
    60661 tgagagcagg acgtcgagcg gatgcgcagc gggaatatga ctggttacgc gccatggtgc
    60721 cgacgttcga agggatggac gagttggcaa aacaattggc cgaatcagaa cgcccgttgg
    60781 taccgaacca tgccgcatga gccatcgccg gcgccccgcg atctggtgat tcagatggct
    60841 cgactgggtg atctggtgca aacgctcccc gccatcgaat cgttgcagga ggcttatccg
    60901 aaacgcactc tggatgttct ctgtgcggca ccgttgagca gcctccttgc cggagtgagt
    60961 catatcggcc gagtcattcc ctgggacggg gcacaatggc gggattgggc cactgggtgg
    61021 ccagagaatc cgacagtcac actacaggtc atgcaacagt atctcgcatc gctcggcgac
    61081 acgacgtatg aacgggtgta cccgttaaat caacatgaac gaagccatct gatgacgcag
    61141 ctgttcctga gccattccac gcgcgaacag gagcacgacc gcatggaggc gcgcatcctt
    61201 ccatgggcac agcatctccg gcacgtcgcc agggaacggg gggccaatcg cgtgcatctc
    61261 gctgatgcct ggtgcggcat gtgtggagtg ctgccgcgcg gccaagcgcc gtcgtgtgag
    61321 ccgccgcccg tcgaactccc gcaagacctt gccgaaatcg gtgagcgcca ggggcgctgg
    61381 attgcgttgg tcaccggagc aggggaggcg gatcgttgta ttcctcccgc cgtgtggagc
    61441 caatggattc gtgagttcct cgcacagact caggatgggc aggtcgtgct gatcgggagc
    61501 ggtacggagc gtgaaaccgc gcaggcgatt ctcgattcag ttccgacatt gctccacggt
    61561 cgcgtctggg atgcgactgg tcgtacgacc atctcccagt tgatgcgtgt gctgcagcag
    61621 tgccactggg tcattggcgc tgatacgggg ccgcttcatc ttggcacgct ggcgggggct
    61681 cgagctctcg gctggtattt ttcgcgtgcc cgaatgcacg aaaccggtcc ctatggtgaa
    61741 gggcattggg tctatcagca tgccacgcag gcacagcccc aacgttggcc gatccaggaa
    61801 agcatcgcat tgatgtgcgg cgaccagggt cgtcgtgcaa ccgattggac cctttggagg
    61861 agcggtatgg atcgatgggg agcgtatttc gatgacggat ccggaagcgc aactgtcgag
    61921 tcctcgcgtg ccgagatctg gcgagcttgt gcaccggccc tttgcgaatc gatggcggca
    61981 tgaatatgtt tcaagacaat ctgaatcgcc tcgcttcacg ccaccgggat ctcgcggcag
    62041 cggtgaaatc tgcagccggc ggagtgctca cgatcgagcc ggcccgaacc ggcctaccgt
    62101 ccgcgcgtcg ttccggtcgc tggattcaca gcgcttacga cccgcttcgt gaagcgcaga
    62161 cctgggcgga tacccatgca gcggcctgca aatctggtga aatggtcgtc gtagccgggg
    62221 tagggctgct ctatcatgtc gaggcgcttc gaaagaagct gacgactgat gtctctctcg
    62281 cagtcctgct tcccaacctc gacgagtttc acgatgccct ggtgactcgc tcgctcgaag
    62341 gctggagtga gtccattctc tggcttccag gaacgccggc tgagattgcc gagacattgg
    62401 caacgatagg ccggccgata cgctgtctgt cctatacgcc ggctgctctt gctgatgcag
    62461 agttccatcg cgccgttgaa gacgcgttac gtcaggggat tgcacgccag gccggtggcc
    62521 aattgaccat tgccctcgtg gggcccattt acggagggtc gctgccgatt acgggctacg
    62581 tcaaacgcgc cctggaaacc ctcggtcata aggtacagct catcgatcac agcatccatg
    62641 cggcgagtta tgaggtgatg ggcgcgctca aagacccgcg caaccgtcaa ttgatgcaag
    62701 gccggatggc cgatgtgttg agccaatgga ccttagccac gctcgccgag tcgcctcctg
    62761 atctcgtgct ctcaatggcg caggctccca tgacattgca ggtattggag cacttgcgca
    62821 agaagaaatt cctgacggct atgtggttcg ttgagaacta ccggcatctc acgtactggc
    62881 agcagttggc gccgggatac gagttctggt ttgtctttca acagggggca tgtctcgacg
    62941 cattccgtca ggccggtgcg cgccaggtga attatcttcc gatggccgcc gatgcggagt
    63001 tgcattgtcc gatgtcgtta tccgaggaag accggcagcg gtttggggct gatgtctcgt
    63061 ttgtcggcgc gggctatgcc aatcgacggc gtctttttcc ggcgctgctt cgccagccct
    63121 ggtcgttcaa actgtggggg aatgaatggg atggggcaga cgacctgcgt tctgttctcc
    63181 agctggacgg ggccaggatc gacaccgcga cgtgcatgaa ggtgttcaat gcgaccgcga
    63241 tcaatttgaa tctgcattcc accacaggcg caggcctgga tccccaagct gacttcgtca
    63301 atccacgcac atttgaattg gccgcctgcg gcgcatttca actggtcgat catcgctccc
    63361 agcttcgcca gttcttcacg gagcaggaga tggtgtcgtt ccaaaatttt ggtgacgttc
    63421 ccggattggt gcagcagtgg ctgaatgatc acgctgcccg ccaggccatg gcaaatgcgg
    63481 ctcgagccca tgtgctgagc gaacatacct atgtgcatcg gatgcgggat ttgcttggac
    63541 acatcggctt gtctcagccg gaccgggtcg ggtccgtcct ccgtggcgag cgccagcagg
    63601 aatcgctcgt gaagcgctgc gccgatgatc gtccgttgga ggcgctcatc caagactgcc
    63661 ccagcgggca acgaatcgag ctgaaggacc tggctgcccg tattcgtagc aaaggtccga
    63721 ccgccaccct caagcgtgag gaactgatgg tcctcatgtt ggatgaatac cgcagcgaga
    63781 cccgcgacct cttatgaaca agcgcgtcct tctgatcaac atcacccgga tgggcgatct
    63841 cgtgcaaatg ggcacgctgc ttcagcggct ccagcacgaa tggccagggg cagaggtcga
    63901 tctcgtcgtg gatcaacggt ttgctcctgt ggcgaagctg ctgccgcacc ttcgacacat
    63961 cgtcgagtac gattttcatc gactggtgga tgagagtcgc gctcaaacca aagatgtggt
    64021 gaccctctat cacgatatga cgcggtgggc ggcaccgttg gttgaggccc gctatgaccg
    64081 ggtcgtcaat ctgaccttca atcggcgaag cggactgctg acgtcctatg tgggcgcgaa
    64141 ggaaattcgc ggtatcgccg ctccgaaaga cggagatgtg accattcaca acccctggat
    64201 ggcctatctg accgacgtgc atcatgagcg tcgcttcaac catttcaacc tcgtcgatat
    64261 ctatgccttg ggcggcagtg gcagtggacc gtttgcgccg ctctcactga cgattccgcc
    64321 ggatacggct gaatgggcgc gcaacttcct cgcaactcag ggcggacagg tacggtcctg
    64381 gatagcggtg caggtcgggg ccagcgatcc gatgaaggcc tggcgtcctg aactgttcgg
    64441 acaaaccttg gcggctctca gccggcaaac cacagccggg tatgtattta tcggcacaga
    64501 agaagaacgg aaagcgattc acctcgctca aacgacatac cggaaaagtg gcggaaccgg
    64561 tccgctctgc gacgcggtgg gaaagacgtc gttaccgcaa cttgccgccg tgctctccca
    64621 gtgccgactc ctgctgacca atgacaccgg ccccatgcat ctcgcagtgg gagtgggcac
    64681 accggtcatt gacctgtcgg tcgggcatgt cgattttcgg gaaaccggac cctatgggcc
    64741 cggtcattgg atggtgcagc cggatctggg atgcgctccc tgtggattcg accaggtctg
    64801 ctttcatcat gcctgcaaag atcgcatcgt gccggaccag gtcgctgcct tgtgtctgca
    64861 tgtactcgcg ggagtcccat ttccccggtc gatctcaggc gtgaggatct accgctcctg
    64921 tgcggatgaa gatggcctgg gaaacaccga actgcaagcc ggacgggaag acctgctggt
    64981 cgattggtat ggtcgctttt ggcgccgttt ctggtttgaa gaattcaccg gccgtcccag
    65041 ccgggttcct tgcgatcacg aaccgtcacc cgattgctcc gacgctctcg ctgtgttgga
    65101 gcacctgaag cctctggcca agaagctggt gtcgaacgca gaagagctgg ttcgtctcac
    65161 gaccaaacgt ccgttgccgg ttagcgcctt gcaacagatg cagcgtcttg aaactgcgga
    65221 gcggcaacag atcgttgcgc tcagcatgca atcaccggcc gccgcttccc tggccgtggc
    65281 catggtacgt gatacccaca atgatgacgg gcacgaactg accggactgg cgcaatcccg
    65341 actcgctacc tatcggcggt ggcacaagcg attgcaggcg gtgtcgaata gtttccgttc
    65401 cttcaccgca gcgcagggat cagtgcgtcg gggaaagacc cgacagctcc ccatggcgcg
    65461 ttccgcataa cgagagaaga agacagcacg aattctttta caggagcctg acatgcatat
    65521 cactcttgat ggcgagcagt tacaggtacc ggaagacacg tcgatgatga acgcgcttgc
    65581 gacgctgagc gataaggcgc atgccaaaca tcatatcgtg acgtcattga ccattggggg
    65641 aaagacgatc agcgatcgcg atctcactcc gccgtttctc aatcaaccgg ttcgtgatgc
    65701 gggggccata caagccatct cccagtcact gcacaccatc attctcgagg cgaagcaggc
    65761 catcgagcga tttgccgccg agctgcgatc cgacgggcag gtgttgctgg cccccttgcg
    65821 ttcaggaacc gcccaggtgg gaaccattga tgcctggctt ggccgactgg ccgattacac
    65881 cgaaatgttg gaagccggac aggcccaggg tgtcgaaggg ttgtcgtctg cctcgttgct
    65941 cccatggatc caggaactct tgggcgcacg aaccacggcc gacgcgatcc gcgtggcaga
    66001 cctgctcgaa tacgaattat taccccggct cgccgatgcc cgtttggctg cttaatatcc
    66061 attgttgctc aatagggcac accgccccgc gctgctcctc gttacaggca ttttctgcgc
    66121 atccttcacg acttaagtct tcctgagagc ctaccgataa ggggcacagc aacacggtaa
    66181 taaggggcag cggggatgtg gccacatctc caccggacct ggttaccacc tcgtcggcac
    66241 ggacgctggc gattccgaga gtagtgcgga agcttcagga ggaagtacgt catggcatta
    66301 gtagtcaaca ccaacgttgc gtcactggca gctcagcgaa acctggccgt caaccaagcc
    66361 caattgggcc gttcagttga gcggttgtca tctggcttac ggattacccg tgcggccgat
    66421 gacgcggccg gattgggcgt gtctgaaacc ttgcgggcgc agatccgtag tatcaatcag
    66481 gccaatcgaa acgccggtga cggcatcagc ttgacgcagg tcgcggacgg cgcggcggcg
    66541 acgattggca gcctgttgtc tcgtatgcgt gagttggcga cccagtcagc cagcggcacg
    66601 ttgggcacaa cggagcgttc gtatctggac caggaattcg tggcgttgcg ttcggaaatc
    66661 gatcgtattt cgaccacgac cgaatataac ggtcagccgc ttctcagcgg tagcagcaac
    66721 acgtttgagg tcttcatcgg cttcaagagt ggatcgggca attccttgag tgtggccttg
    66781 gatgacctcg acgtggcggc ggtcggtttg accggcgcaa gcgtgtcgac ggcaacggcc
    66841 gcacagtcga tgctggcgaa gatcgacagc gcgatcagcg cggtcgctac cgcccgcgcc
    66901 aattacggtt ctatccagag ccgttttgaa gtcgcgatcc agaatctgac ggtcacggcc
    66961 gagaacttca ccgcggccga atcgcggatt cgcgatgcgg acattgctca ggaaacatct
    67021 gtgttcacga agaaccagat cctcactcaa tccggtatcg cgattttggc gcaagccaat
    67081 tcgctgccac agcaagcgtt agcgctgctg cgagggtaag gcttcagccg gcgggtagct
    67141 ctccataggg agggctaccc caggactgat ccgaggaggg taacatggtc cacgatgttt
    67201 ccaattataa agaccttccc gcagccgcta ttcaagcgac gcaaagcggg cctcagcata
    67261 cgactgaaaa gaaggtcgag gcgaaggctc ccgaacctga gtcgtcaacc cctgagacga
    67321 agggaaaaga tctggaacag gcgctcacgc gcgtgcgtga ggtcttcaag aaggcagact
    67381 ccaggctgga gtttaccgtc gatccggatc tcgatcgggt agtggtcaag gtgatggatg
    67441 gggattctgg taccgtaatc cgccagatac cgcagcagga agtcatcgac cttgcgaaga
    67501 ggctggagac ccccacgggg ctgctcctgc accacaaggt gtaagctgca tcacggcatg
    67561 aaagggatgt catggcgatc agttttggcg ggttaggcaa tggcgtggac tttggccagg
    67621 tcgtcactga gctggctaaa gtccaacgtg tgcctatcga cgcgttaaac gcgaagaaga
    67681 aagacctcca gactaaactg accgactacg ggaccctggg caacaaactg ctcgcactcc
    67741 aaagcgcagc caatgcgctt cgcctcccaa acagttttga ccgcacgacc accaccgtca
    67801 gcaacgagac caaattaacc gctcaagccg gcgtgggcgc ggcgaccggc agctatacgg
    67861 tccaggtcac tcagttagcc aaagcgcacc agattaccaa cagcactgcc aaggcggtcg
    67921 ccgcgacgac cactccaatc gtctcaggcg gctcagggac ctttaccttc aaggtcggaa
    67981 ccggcgccaa tcaaacggtg acgctcaacg atggcgctac gctcgacgat cttcgttccg
    68041 ccatcaacga tctgggagcc ggtgtcaccg cctccgtgat caacaccgga actgtatcca
    68101 caccggccta tcgactgacg ctcacggcca ctgctacggg cgctgccaat gccgtgacgg
    68161 ttgtcacaga caccaccagc ttagacttca ccaacacgag cggaaccggc ggcaccagta
    68221 cgctgcaagc aggacagaac gccattgtgg tgatcggtga accgggtcag acgacgattt
    68281 ccatcgagcg tgaaagtaat gtgatcagcg acgccattcc cgatgtcacg ctcacgttga
    68341 aatccaagac cgtcaccact cccacgccgg aaccggtcac cgttaatatc acgaccgatc
    68401 cggacagtgt caaaaccaat atcaaagctc tggcgaccgc ctacaacgat attgtgaaat
    68461 tcgtcaatga gcgcacaacc tatgatatct cgacgaagac cggcggcatt ttcttcaacg
    68521 agagtacggc gaaaagcgtc ctgagccagc tgcgaactgc gctttccgga gaggtcagcg
    68581 gactgtccac gtacaaaagc gtgggaaccg tcgggtttaa aacggaacgc gatggaaccg
    68641 tcacggtaga cgacgccaaa ttagatgccg cattggcgag caactacagc gccacgaaag
    68701 ccctgtttgt cacacaaaca acatccagcg gcattgcgga ccgggtggtc aaggccgtcg
    68761 atttcctcga tgccatcgat accggcgcct ttacggtgag aaagacagct atcaccagtc
    68821 agatcagcaa actcaccgat gaaatcggtc gcaaggaaga catcgcctcg caatatgagg
    68881 agcggttgcg actgcaattc gcctccctcg atgcattact tcaaaaactc aaaggccaaa
    68941 ccagcgctct ccaggcgtta caataaccag ataaggtcac catgattgct acggcagcta
    69001 acgcctacca acagactcaa gtcatgacag ccagtcgggt acaactcatc gtgttgctct
    69061 acgattctgc gattcaatcg atggagctcg cccgggaagc cattctcacg aacaactaca
    69121 aggataaggc ccgattcctc gatcgcagca tggccatcgt cggagaattg tccagcgtcc
    69181 tggatttcga gcgaggcggg gagatcgccg tgtccctgca ccggctgtat gactatatgg
    69241 tccagcaatg catacaagcg aatcttcggc ataacggcaa acacttggat ggaccgattc
    69301 gctgcctgac gaccctccgg gaaggatggc aagtcgtcgc acgacaagaa gcggtggcgc
    69361 atgtcgccta gttcctccca tcccggttcc cctcacgacc gtcgctccat cgaacaactc
    69421 accatccaag cactgaacgc cgcccgagca ggggactggg atcaagtcga cgcctgttat
    69481 agcgagcgcg gactcagtct tgccgcatgc aaacctgatc acaccgttgc acaacagctt
    69541 ctcatcatgg atgaagaaat tcgccaggcc atcctgctcg cgcaagctgg aatctccgct
    69601 ctcttggctg aggcctctca cgtgacccgc cacctgcgcc aattacgcga aagtagcgga
    69661 caactggcct cagacagtgt aacgatacat cgagaagcct agcgttcttg cagccagata
    69721 ccctggcttc cccgtgacct tccactagag aggtttggta tgaggccttc acctgagcgg
    69781 ccgctgcagc gcccgcatca cgacatcaag aacgaagagc ggcgcgagtg gctccgcatc
    69841 gacgatcgcc ttctgctaga atatcgacga gccgacgagc cgctggaggc gatgaaccaa
    69901 tatttgccgc cggcaacgga agacaccatt gccacggccg tatcgaagcc aaccgttgat
    69961 ttgctggtac gggcagggga gacgtttgcg ggttctccgc tcctgccgtg ggtatcaaaa
    70021 atcgactgga tgctggagac gatcctcaag tcgatggtca agagtcaccc gggcagtgtg
    70081 agcatcgcgc gactgaccga tgtggatatt agtgcgggcg ggttgggatt caacacacct
    70141 cgcgaatttc aggccgatga cctgctgatg ttgaaagtga tcctgccgcc gttcagcatg
    70201 attgaaacca tggccaagat catccgggtg acgccgctcg atgaggggaa ggcaggattt
    70261 catctcgcca cccagtttgt cgatctgggc ggggacgagc aggaactgat tatccggcat
    70321 atcctccaga ttcaagccga gcggctacga acgaggaagg ccgcgcataa tcatctctga
    70381 atggtgagca ggatttgagg cgctaaccgt ctaggccgcc cgttccggag agggagcgga
    70441 tggctgcggc atcgtcgctg gtttggtgag ctgagccaag agcgcatcag ccgctccgtt
    70501 gagggcgacg gaagccggcg cggtcggttc gaactccacc accggcaaat agctgcgaac
    70561 cgcccggacc atcgccggat cgtccggaat ctcgcccagc atcgtcaggt ctcccccgac
    70621 atactccttc agcagctttt tcaattgcag cacgttgacc cgtgatttgc ctgacacgcg
    70681 attgacgatg aggtagggtt gaaacccttg taaggtgtta ctcgccgtct ctttcgcatt
    70741 cggatctgtc tcgcccacgg cctgaatcac ctcgtcgata ctggcgaaat cgcgatcaga
    70801 cagcgcttcg ctcaccgcgt cacgggacaa gaatgccgat aatacacgtc gaatggcggc
    70861 cagcttaatg aaccggtaga gatcaaggac cgaggtcgga tctggtgtcg ccaccgtgag
    70921 atagcagtcg gccatgagaa aaaaatcgag cgcatgataa ctggtgccgg cgccgatatc
    70981 gacgatgatg acatcagcct gcaactggga aaagtggcga atcaggcgtt ttttcttggc
    71041 ataggggagg ttggcggtgg ctaacgtatc cccggtaccg ggcaggagtt gcaggaaagg
    71101 atgggccgaa ataggctgaa gcacctcctc caaacgctcc acccgccgtt caacaaaatc
    71161 ggtcagggtg cggggagggt tcagcaaccc gaacagaatg tgcgcgtcgg caccgccgac
    71221 atcgagatcc gccagcacaa cgcgcttgcc ccgtttagcc aatagcatgg ccaaattggc
    71281 ggcaatgacg ctctttccta caccgccctt ccctgatcct atcgatacga tggtggccat
    71341 ccgcaactct ctctcagctg tgtgtgttcg ccgatgtctg ttgtcggtcc ttcggcctag
    71401 gaacctgagc ggaataagcc catagggaga atatctccct gcgactccag cctgtgcctt
    71461 attaaacccc atcgtccaag aaccggcaac taaagtttga gatggcgccc accgatacca
    71521 tatccgttat gaacaacgct attgcgccca caaccaacgt caacttcgac agtgtatcgc
    71581 cttcggaagg cgagattctc accgtgtcgg acgtcgcgcg gtttctgcgt gtacccaaat
    71641 ccacggtgta taaactcgcc cgtctcggag aattgccggc ttccaaaatc ggaaagcact
    71701 ggcggttcct ccgacgcgac attcacgagt ggatgcacag ccggacccag cagcactgaa
    71761 ctctcgttgg agagcagctg cagagacgac aacacgtgag cagcgcggac tcccgggatg
    71821 tccgttgatt gagctcacct ttagcggatc acacgaggta agggatggcc gagtcaaacg
    71881 cggtgcgcaa aacggaaatg cccgccccga agctaagccc ggacacattc aagcagctcc
    71941 gggatctcat ctatgagatg accggcattc actttcaaga caacaagacg tatctgcttg
    72001 aaagtcgctt gcttcctcgg ctgaaggcct gccgctgtca gacctttgaa agctatctca
    72061 actttttgcg cttcgatgcc taccgggacc gtgaaatcac cgagctctat acggtcatca
    72121 ccacgaatga aacatacttt ttccgcgatg aagcccagct cgatagtttc atgaaggtga
    72181 tgattccgga agtcatgaaa acgaacgccc agacgaaaca attgcgcatc tggagcgccg
    72241 cctgttcgac cggggacgaa ccgtatacct tggcgctgct gctgcgtgac tatcctccgc
    72301 tgaacggctg gaccatcgat atcctcgcaa ccgacatcag tgaaaatgtt ctgaacatcg
    72361 cacgaaccgc cacctattcc tcccattcgc tgcgcaaggt gccgccggcg atgcttgcgc
    72421 gctacttcac cgggaagaaa gaagaacaga cgctggtgca accggtgaaa gacatggtgc
    72481 ggttcatgaa cgtcaatttg tacgaccgac ccagactgaa gctgatccgc ggcatcgata
    72541 tcattttttg ccgcaactgc ctcatctatt tcgacgaaaa agccaaggcg cagattgtct
    72601 cggatctgcg cgacgccctt cgtcccaagg gatacttgat gatcggattt tccgagaccc
    72661 tgcacgacgc ggcaggccac ttcaaaacca ttcatgcggg ccgttcagtc attcatgaga
    72721 aacagtaagg aggacccatg ccggctgatc cgaatatgaa gattctcgtt gtcgatgaca
    72781 tgtccaccat gcggcggatc gtcaagaaca tcctgaaaca acttggtttc aacaacctgg
    72841 aagaggcgga aaacggtcaa gaagcgttga ccaaactcaa agccgacacg tacggcttcg
    72901 tggtgtctga ctggaacatg cccgtcatga tgggcatcga catgctgcgc gccattcgcg
    72961 cggatgaatc gctcaagaaa attccggtcc tgatggtcac ggcggaagcc cagaaggaaa
    73021 atctcatgga ggccgtgcag gccggcgtga gtaactacgt cgtcaagccg ttcaccgcag
    73081 agacgatgca ggacaaaatc aataagattt tcaagtaatc ggctcttaac cggtgggggg
    73141 gacgatggca caaccacgcg cggcggccca gtcgcaacca gcgacgcatg aatcagagca
    73201 ggaagaaaaa gcacctgata ccaagctcta tgaggaactc ggagaattgg cccgcttcat
    73261 cgacaccacg atgaagacgt tgtccgaatt cagcacgccg gtcagcacct cgaccgagca
    73321 gctgccgcaa gcgctcagcc acctcaccaa tctcaagacc atgaccgagc agggaactca
    73381 tcgggtcatg ctcgaggttg aggccattca agacaatcac atgaaaatca gcggcatgat
    73441 caaggacctg acgctcgcca tgcagaaggc gcaggcggcg cccgcactcc tccagcagat
    73501 gcaagccgtc ggcaatgcca tcaacgcgga cgacaagcgg ctgctcgata tcatgacggc
    73561 cttgtcattc caggatctgg tggcacaaag cgtcaacaag ctcgtcacca tcctgcaaga
    73621 agtcgaacac aaactgctcc aactcgtggt ggtcttcggc ccctatcaaa agcaggcggc
    73681 aaaaaaagag caggacaagg ccagcgaaat gctgaagcaa ttggaagcga ccaagaatac
    73741 atcgatgaat caggatttgg ctgacgaaat cctgaagcaa ttcggtttta actgaggtgg
    73801 gttatgagcg atgaaatgca ggaaatcctg aatgactttc tgactgagtc caacgagatg
    73861 ctggaagtgt tggaccagcg cttcgtgacg ttggaatccg atcccaacaa caccgatctc
    73921 ctgaacgaga tcttccgcgc gatgcatagc atgaaggggt ccgccggctt cctcggcttc
    73981 aaccatctgg tggatgtggc ccatcgcggc gagaacattc tcaacaagct gcgccagggc
    74041 gaaatggccg tgaacccggc catcatcagt atcattctcg aaaccatcga cgtcatcaag
    74101 gcgatcatgg ccgacattcg ggaatcaggc actgacaacc acgtcgccac ggcatcgatt
    74161 gccgcaaagc ttgacgacat tctgaacggc aacacagccc cggctgctgc gccggtacag
    74221 acggaagccg ttgcgccccc gaaagtcgaa gccgcctcgt cggcacctgc cgcggccgct
    74281 ccggtcgctg ctccccagac gctgggtgaa attctggtga acgacggcct ggcctccaaa
    74341 gagcaggtcc ttgatgccct tacggcgcaa cagcatcaac cggaaccgaa gactccgctg
    74401 ggcgaaattc tcttgcaggc aaaagccatt accgaacgcg ccctcgatca agccctgcac
    74461 aaacaagata aacagccgaa gccggtggaa gaagatgcca ccattcgcgt ggaaacgaaa
    74521 cgcctcgaca gtgtgatgaa tctggtcggc gagctggtcc ttgggcgcaa ccggttgatc
    74581 aagatcggca cccaattgga gcagcagcac gaatctgatc cgcaggttcg tgtgctgagc
    74641 gaaacactcg cgcaactcaa cctggtgacg accgacctgc agttagccgt catgaagacc
    74701 cgcatgctcc cgatcaaaaa agtgttcgcc aaactgcctc gcatggtgcg cgacctttcg
    74761 caaaaactga acaagcaggt tcatttggaa atgcgcggag aggaaacgga gcttgataag
    74821 tccgtcgccg atgagatcgg cgatccgctc gtacacctgg tgcgcaatgc catcgatcac
    74881 ggaatcgaaa ccccggccga gcgccaggcg aaaggcaaag caggggaagg acaactgacc
    74941 attgcggcga gccaggaagg caacagcatc gtcattcgga tcaacgacga cgggcgcggt
    75001 attcaggtcg agaaggtcaa ggagaaggcc ttggccaaag ggctgatcag cgaagccgaa
    75061 ctcgcgacga tggagcaccg tgaaattctc aatctgattt tcctgccggg attcagcact
    75121 gccgaaaaag tgacggatgt ctccggacgc ggcgtcggca tggacgtggt tcgcacgaat
    75181 atccgcaaaa tcaacggcag cgtggatctg gaatccgagc cggggaaggg cagccagatc
    75241 attatcaagc tgccactgac gatcgcgatt attcaggcct tgatggtgga agtcgagcgt
    75301 tcgattttcg ccattccgct gagcacggtc attgaagccg tgcgaatttc tcgctctgag
    75361 atcaagacca tcaacgggcg tgaagtcctg cacctgcgtg accgggtgct gccgctcatt
    75421 cgcctggctc aggagtttga cattccgacg gacgccaatc gtgagcgctt ctacgtggtc
    75481 gttgccgcat tgggagaccg tcgagtggga gtggtggttg atgagttgcg atcccaggaa
    75541 gaagtggtga tcaagtcgat ttgggattat ctcgaaaccg tcaagggcgt atcgggcgcc
    75601 acgatcaccg gcgaaggcaa ggtggtgctc attctggata cgtccgagct ggttcaaaat
    75661 gcacaagcgt ggcataaccc gggcatcgcc gcctgacaca agggtgtatg ccctgagctg
    75721 tgggcgggga gacgggttca agaatagtcg atgaactgag acgcgcgaga tcggaggaag
    75781 tatgtccaca ctgatcaatg aaatcgatgc caggaccagg ctagccggcg caaaccagat
    75841 ggaattgctg ctgttcaagc tcgggacaaa cgagattttc ggcatcaatg tcttcaaggt
    75901 gcgtgaggtg atgaagctgc cggagctgac ccagattccg gaagcggaca accgcatcgt
    75961 cggcatggcg aatatccggg gcatcatggt ccccgtcgtc ggactgaagc gaagcttggg
    76021 cctgggaatg gaaaccgagg gacagggcaa cggggctccc ggcgggaatc cctacctgat
    76081 cgtctctgaa tataacggca gcttgcaggg ctttctcgtc gccggcgtgg atcgcatcat
    76141 tcgcttttcc tggtcggcca tcaagacgcc gccggccatc gtccgggaga acaacaaggg
    76201 agccgtcact gccgtcacga tgctggaaga cgggcggatg gtcttgatcc tcgacgtgga
    76261 aaaagtcttg aacgacattt gcccgcgcac ggacgacgat gtcttcgccg ggatgacgat
    76321 cgcccccgaa ctccgattaa agtgcgtctt gtttgccgac gattcttcag tggcccgcat
    76381 gcagattcgc aaagcactgg atcgcctctc catgtcgtat gtgattgcga cgaccggcgg
    76441 cgaagcctgg caaaagctgc aagcgttggc ggatcaagcc accgcagaag gtaaagagca
    76501 agtggatcag atccagtgca ttctcagtga catcgaaatg cccgagatgg acggctttac
    76561 cttgaccaaa cacatccggg ctgatccgag actggcgcat ctgccggtca tgctgcactc
    76621 ctctctgacc ggtgcctgca acatggagaa gggacggacg gtcggcgcga cggattacat
    76681 cacgaagttt gacgcaaaaa tgctgggcga gaaactggcg ctctacattc aaaacccgaa
    76741 cgccaatgtg aaaaaagcgg cctgatatcg ccgcagaccc tggcgtcagg gtgacggctg
    76801 attcaagctt ttgtcagaga cggccgatgt atcgctaaag atcctatggc acttccactc
    76861 acacagccgc agagcgccgc gaagccggtt cgcgtcttag tcgtcgatga ttccgcattc
    76921 atgcggaaaa gtctgaccac gatgctcgaa gaaggcaaac agatccaggt ggtgggagtc
    76981 gcacgcaacg gtgaagaagc ggttcagcag gtacaacagc tcaagcccga cgtcgtcacg
    77041 atggatgtcg aaatgccggg gatgacgggc cttcaggcgc ttcagcagat catgaccaag
    77101 aatccagtcc cggtcatcat ggtgagttcg gtgacggtcg aaggcgcgca agagacattg
    77161 caggcgttgg agtggggggc ggttgatttt atccccaaac aactcgatgg cgtggcatcg
    77221 aaaatcgccg agattcaaaa aatcctcgtc cccaaggtgc tcgcagccag atatgcgggc
    77281 agcaaactga aacgactgaa cgttacaggc gcaccgaagc tcgctgtgcc agccgccaaa
    77341 gcgttgagca gtcgctccgt ctccgtaaca cgcggcacca aactcattgc gatcggatgt
    77401 tccacgggtg ggccacaagc cttgtttgaa atcgtcccga cgatccccgc cgattgtccg
    77461 gccggcatcg tcatcgtgca gcacatgccg agttcgttta ccaaaccgtt cgctgaacgc
    77521 ttgaacaatt tgtgcgcatt ggaagtacgg gaagcggttg acggcgatga ggtgaaagcg
    77581 gggcgggtgt tggtggctcc gggcgggatg caattccggg tcgtgaagaa gtccatcacg
    77641 acgtcagtcg tcaaattgtc gccgaattat gaaaggcatg cccattcgcc atccgtcgac
    77701 atcatgctcc agtcggtcgc cgcattgttc ggagagcgca gcatcggagt gattttgacc
    77761 ggaatggggc atgacggact ggaggggatg aaggccatca aagcagcagg aggtcgaacc
    77821 gtggcgcagg acgaagcgtc aagtgtcgta tacggcatgc cgaaagccgt ggtcgaagca
    77881 ggatgtgcag agaaggtggt gtcactttca aaagtcatcg gcgagatcat gaacatggtg
    77941 tgacagggat cgttcattca accgttcacg catggacagc taggaggagt tatggggagc
    78001 atgatgaagc acatgtcgat tgggccgaag tttattatat cgatttccgc agtagcccta
    78061 gtggtgatca cacttgggct tgtggtcctg tatcagcagg aagaaacgaa gatcgacacc
    78121 ctattgactg gtcgaatgaa tgtggtcagc acgcagatca tgattagccg cgcctatatt
    78181 acccagaatt atgtggcaaa aattaagaaa tccaaggccg gcaatgaaat ccaggtgctg
    78241 aaagaccata caggcgtggc cgatgccatt ccgttccccg caacggccgt tcgtgaaatg
    78301 ggtgaagagg ctaatcgcac ggggttatac aacgtacggc tcatcagtca gaaccccatg
    78361 aatccgtcca atacgccgaa agacaatttt gagaacgagg cgattcgggc catcatgagc
    78421 ggcgccgaat cctatgcgcg ccgggacgaa gtcaacggcg tgccgacgtt ccgtcgtgcc
    78481 accgtggaca aagccacttc gacggcctgc ctgagctgcc ataccaacaa ccaggttggt
    78541 gacgtgttag gcatgctgag catgtcggtg ccgatgaccg aagcgatcgc attgtcgaac
    78601 cgctccatgt ggcagacggg cggcctgatg ggcgccatcg tcgccatcat tctcgtcgtc
    78661 atgtatctgt tgctgcgcaa catcgtcttg cagccgatgg ccaagatgag cgagatctcc
    78721 cgcgacattg ccaagggcga aggcgatctc acgaagcggg tgccggccga aggccatgac
    78781 gaaatcgcgc agatgggcca gtacttcaac gaattcatcg agaagttgca gcacatgatc
    78841 aagaaggtgg ctcacgtgac ggataaggtc gcgtccgcct cagtcgagct gtcggccacc
    78901 gccgaggaga tctcgaaggg caccgatacc ttgacgtccc gtgcgtcgca gacagccgcc
    78961 gcggtggaag aaatgaatgc cacggtcgga caggttgcgc agaactccgg caaagccgcg
    79021 agcctggcgc aggatacggt caagaccgcc caggagggtg gaacggtcgt gtcgagcacc
    79081 atcagcggaa tgcagcagtt gtccgaggcg gtgtcgaact cggcgaccat catctctgat
    79141 ctcggcaaat catccgatca aatcggcgaa attgtccgca cgatcgaaga catcgccgac
    79201 caaaccaact tgctggcctt gaacgccgca atcgaagcgg ctcgagccgg cgaacagggt
    79261 cgtggattcg ccgtcgtggc cgatgaagtc cggaagctcg cagagcgaac gaccaaagcc
    79321 acgaaggaaa tcggcgatat gatccgccag atccaacagg atacgcgcgg agccgtcgat
    79381 tcgatgcagc agggcacgca gaaagtcacg gcgggtgtgg acctggtcaa caagaccggc
    79441 gaagccctct cccagatcgt gcggatggtg tcggaaagcg ccgatatgat ccgtcagatt
    79501 gccgtggcgt ccgaagagca atcggttgcg acgcaacaga ttgccagcga tatcgaaaac
    79561 gtggcgaagg tcacgaaaga gtcttcgtcc ggcgcacatg agtcggcaaa ggccagtcag
    79621 gacctcagcc agttggcggt cgaattgcag gggatcgtgg gtggattcaa gctgtaagca
    79681 gccaaccacg agaaggagcc cagcatgagt gcgactgaac aacaagtgca atctcacatc
    79741 caggcggccg cagaaaattc tgcggaccgt ctggtcgaga agtccgccga cgatctgctg
    79801 cagttcgtga tctgcctgat cggcagtgaa gaattcgcgg tggatgtctt gagcgtgcag
    79861 gagatcaacc ggatcgtgga agtaacgcga gtgcccaaaa cccctcccta tgtggaaggg
    79921 gtgatcaatc tgcggggccg catcattccc gttctggatc ttcgtaaact cttcggcctc
    79981 accggggcac agcagaccac tcagacccgc atcgtggtcg tatcggtaca ggcgcgattg
    80041 gtcgggctga tcgtcgattc agtggaagag gtcctgcgtg tgccgaaaag tgcgattgaa
    80101 ccaccgccct cggtcggtac catggccggg gcggagttta cgcagggagt cggccggatt
    80161 gatgatcggc tgctgatcct cgtggatctg agccggcttc tcttggcccg ggaagcagcc
    80221 taagccggaa ggatgggcag tgaaggcctg ccttctgcat ggggagtgag cacgaatcag
    80281 gactggggaa catcgggcga atcgggtagg gagggtcctc ggcgattccg tgcgtcgagg
    80341 acccgttcca gcaactgaat cgactccgcc agaacgtccg acgcgcagtt tagggtgaca
    80401 tgaaactgtt gcatggcttc cgtccaacgg ccttccttct gcaagacctg aaacttgcgg
    80461 tgttgttcat cgaggatggc ctgtttggca tcgaggatca agctatcggc agggcccaca
    80521 agaacacctc catggaaaac gtgtgtgatt cggttgcacg ctatcaaagc ggttcccggg
    80581 ggcgcaagcc caccattcta ggtagatggc gaaggccaca gtgcgctggg acggtgaggt
    80641 aactgaactg tggatatcgc cacaatcata ggtattgtcg tagccctcgg ctcgatcatc
    80701 ggtggacaag cactcgaagg gggccatctc ggatccatcc tccaattgac ggcgtttatc
    80761 atcgtcgccg gagggacctt cggcgcctgt tgcgtgcaga acccgctgtc tgttgtgatc
    80821 aaggcggcga ccggggccac cctcgtcatt accaacccac cgcacgatgt gaagggcacg
    80881 atcacgcaaa ttctcgatct tgccaacgtc gcgcgcaaac aaggcttgct cgcactggaa
    80941 ggcaaactca aagatcttca cgacccgttt ttcaagaaag ggatccagct gatcgtcgac
    81001 ggcacagatc ccaagttgct gcaggaaatc ctggaaatcg aagtcgagca tcatgaagaa
    81061 gaaggcgtcc acgcggcgaa ggtctgggaa gccgcgggtg gatacgcgcc gaccgtcggt
    81121 attttgggcg ccgtgctcgg tttgattcac gtcatggaga acctcgccga tccatccaaa
    81181 ctcggcggtg gtatcgcggt ggcattcgtg gccacggtat atggcgtcgg actcgccaac
    81241 ctgttttttc tccccatcgc caacaagatg aagttcaaac tcaaagagga agccggcctc
    81301 cgcacgatgg tgatcatggg gctggtcggt ctggctcagg gcgaaaaccc ccgccttcta
    81361 caagagaaac tcgaaagctt cctccctccg cacgagcgca ccaaggaaga caagaagtga
    81421 tgcgtcaccc cgccagttgc cgccgggaca gatgtacacc gagcgtctgc atgagtgccg
    81481 catagcccgc acgggttgaa gccatggcga agaaaaaaca cgaagaacat gaaaatcacg
    81541 aacgctggct ggtgtcctac gcggacttca tcacgctgct gttcgccttc ttcgtggtca
    81601 tgtattcggt gtcgtctgtg aacgaaggca aatatcgaac ggtcagtgat tcgatcaagg
    81661 cggcgttgaa tccggtgaat agcacgccct ccagccggct gcccttcgcc gtcggcgact
    81721 ccaagcccaa gatgatcaat accgacatgt cgagcccgaa cgatccggtt attcggcgga
    81781 tgaaggaggt catcaaaaag attcatccgt cgcttgcgct ggaaatgccg gacatcaaaa
    81841 tcatcgagac cggaaacggg accatcatga tttccctgcc ggaatccatt ctcttcggca
    81901 gcggggaagc gcgggtccgc cccgaagcgc ttccgttcct gaagtccctg gcccagatcc
    81961 tcattgaaat ggatcggcac atccgggtac tcggccatac ggacaatgtg ccgatccgga
    82021 ccgctcagtt tccttccaat tgggaactct cggccgtgcg ggcggtcatg gtggtccgga
    82081 tcttctccga actgaatgaa gtccccatct cgcatctctc agccacgggc tttgccgact
    82141 ctaaacccat cgccaccaac gacacccctg aaggccggac gaagaataga cgggtagaaa
    82201 tcattgtgct cgagaaaaac ctgacggagg agtccctcga ttcggtcctg ccgaatccgg
    82261 gtcctccggc tctatgacca tggtccaaag gcctcagtcg actccagtgc taaatacaac
    82321 cccttacgtg taaaggtttt tgggagacac accgatacag agtcacgtga ggtagcgacc
    82381 gcctctcgaa aggaacgagt gtatgacgct caagccggtt ggtgatctga cgatttttga
    82441 ggtgggtgcc ttgtgcgagg aagtcaaaca agcggcagcg acccatccgc aggtcgaaat
    82501 cgatctctct gagatcgaca agctggatgc cgccgccctt caactgctca tcgccgttcg
    82561 ccggtcggag cattgtgtcc tgacaggaat gactgatccc atacgaacgc gaatgaccga
    82621 gttgggtgca tagacgtgtt ttaccgcgcg atcgggtaag gaatagatcc accaatgatt
    82681 ttacactggt tattcctatt gtgtgcgttc ctcgccggtg ccttcctctc gtggatgtgg
    82741 tgccaacgcc gggccgctgc cgcccaacag gcgctcgagg cggcacaaca acatattctc
    82801 aagcaacata tggatcgcga cgcgatctgg gaacaactcg gacattccat cattccgctg
    82861 gtggcggtgc tcaacgcaca aatgaaaagt gtgatcaacc aaactgaaca ggccgcgacg
    82921 gacctgggcc aacggttcag cacgattgcg tcgcgggcgt cattccaggc caacgaatcg
    82981 atgaatctgt tcggtgatgg cgagatgaac ctcgatacgg ttctgaaaac cacggacacg
    83041 atgctcgaag gtttcgtgag cgacgtcatg aagtcttcgg aaatggcgct tcagatcgcg
    83101 accattatgg atgaggtcga acgcagcacg aaagccatta ccggcatgat cggcgaaatt
    83161 gagtttattg cggaccagac acgattgctc gccctcaatg cggccatcga ggcggcgagg
    83221 gctggtgaac atggccgcgg atttgccgta gtcgccgatg aagtcaccaa attagccaat
    83281 cgctcgggac aggctgccag cagcatcaac acgatggtca aagacgtgca gagaagcacg
    83341 acccaggcca tgcagaccgt ggaaagtctc gcatcggtcg atttgagcaa gaccttgtcc
    83401 atcaagcatc gactggacca aatgacccgt aacttggcgg atcgcaacaa aacgttgcat
    83461 tcagcggtca tgaattccaa gtcgcactcg gatgaacttt ccaaagacgt cggtcacatc
    83521 attatgtccc tccaattcca ggacatcaca cgacaaaagc ttgaacatgt gatcgaaccg
    83581 ttaactgagg tgcgcgacat catggacgcc ttgatccaaa gacaccctga cgaccagatc
    83641 gccgccagga tgaatattct ggcgaacctg gaccgcagct acaccatgga agaagagcgg
    83701 gccatattca atcagtcgac gaatggacag gcgtcgggag gagctcaggt ttctgcctcg
    83761 tctgaagatg cgaatgtcac gttgttttaa gtaacaccgg ttgtcgaaag aattaaggag
    83821 ctcgccatgg cgaaaacagt attggttgtc gacgattctc ccacgatgcg gcagatggtg
    83881 gcgtttaccc tgactaacgc cggctatcag gtcgtcgagg ccggcaatgg caaagaggcc
    83941 gtcggcaaag tgaatggcgg ggccaagccg gacctggtcg tcaccgacct gaatatgccg
    84001 gaaatggacg gcatcac
//
LOCUS       DAOQDS010000006        78810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_91_length_78810_cov_17.165094, whole genome shotgun sequence.
ACCESSION   DAOQDS010000006 DAOQDS010000000
VERSION     DAOQDS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 78810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 78810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78810
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91_length_78810_cov_17.165094"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PK589_02315"
     CDS             <1..884
                     /locus_tag="PK589_02315"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNJ18456.1"
                     /translation="ELDKTVIESIGDPLTHLVRNSADHGLEPPDERLAAGKSEQGTIR
                     LNAFHEGGNICITVEDDGRGLNRDKILAKGIKQGLIAETDKLSDEQIWMLIFKPGFST
                     AEKVTDVSGRGVGMDVVKRNIEGLGGTVSIKTSAGKGTTFTLKLPLTLAIIEGMTVRV
                     GRDTYIVPLLSILESIQPKREMIKTIVGKGELVNVRGTYLPLMRLYDVFRLEPELSDP
                     TKAILLILETEGERVAVMVDEILGQQQVVIKSMEQNFRKIEGVAGATILGDGTVGFIL
                     DVRGLLNIARQRKAKAA"
     gene            945..1454
                     /locus_tag="PK589_02320"
     CDS             945..1454
                     /locus_tag="PK589_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNJ18457.1"
                     /translation="MASQESASKESNQQIGITTDGSQFLTFQLGEELYGVDILRVQEI
                     KGYTAVTRIPNTPPHIKGVLNLRGTIVPIVELRTKFGMPTIEYTMFTVIVVVVVKEKI
                     MGLVVDAVSDVLDIDKKDIQPAPQFGAQVDVSFLNGIGKAGDKLVALLDMDRLLTDGD
                     LQETEKVAA"
     gene            1482..3932
                     /locus_tag="PK589_02325"
     CDS             1482..3932
                     /locus_tag="PK589_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNJ18458.1"
                     /translation="MALQVELLESSFKLVAPKGEELVTRFYERLFKKYPAVKPLFKHT
                     TIKEQKKKLLASLVLVIQNLRRPEKLTPVLQDMGARHVGYGAKPAHYDAVGENLLAVL
                     GEVAGPAWTPQVKQAWTEAYGAIKTIMLAGAERVHNTQGEKTMSKPKGKKGTAAQADM
                     TEFYLGALDQSQNNILLCDRNLVITYANSTAQKTLATLEPAIKKVLPKFNLSTVVGTC
                     IDDFHLDPSKQRRILSDPANMPHRADIQIGPLTLSLLVTAVMSPSGEYLGNALEWADV
                     TEKRRLETEMSRLKASLDNARSNVLVCDRSYTIVYANNQSVTTLRRLETEIQKVMPAF
                     AVAKVVGSNIDQYHKNPAQQRTLLDNPKNLPYKTEIKIGPLTLDLAVAAIMNEKGDYL
                     GNVVEWADISAQKKAQVEIDKLIEAAASGQLSERINAAEFDGFFKTLSEGVNKMLDAV
                     VTPLHEAQTVLTAMAANDLTKTMTGHYQGEFEQMKSSLNGALNTLSTAISTVRDGAQS
                     VTAGAEQITKGNEDLSQRTSEQASALEETSASMEEMTSTVKQNADNAKQANQLAIAAR
                     DIADKGGSVTVRAVEAMGEINKSSKKIADIITVIDEIAFQTNLLALNAAVEAARAGEH
                     GRGFAVVAAEVRNLAQRSATAAKEIKGLINESIQRVNDGSELVNQSGKTLEEIVSSVK
                     RVTDIIAEITAASQEQAQGIDQVNKAIMQMDETTQQNAALVEETTSASQSMKDQAQEL
                     MRQVEVFKIEAGETQRQSNAHSSVGKSPAKSAIVATKPALKKPSFTARPADAKQPVGT
                     PAGNGHDRHKKDDDFEEF"
     gene            4138..4599
                     /locus_tag="PK589_02330"
     CDS             4138..4599
                     /locus_tag="PK589_02330"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNJ18459.1"
                     /translation="MDVKTLLWYLVLSSLTVAYLVVLAGVKAAKSHDVTHHSHRMIVG
                     CTIVGIWLVAYVLKQALFGRESFQGPESAYWSVYVPIFVTHMLLAVTTIGLGAYNLYM
                     GLHRLRFGSVGAMVAGMTTHRRMGNALIWTFSGTMVTAYLVYLMLFVWYRS"
     gene            4604..5422
                     /locus_tag="PK589_02335"
     CDS             4604..5422
                     /locus_tag="PK589_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HNJ18460.1"
                     /translation="MDYPISKKEYDQIRTLLYDESGISLGESKQSLVVSRLTKRLRDL
                     QLGDFSSYYEYVTEDNSGEEFTRMLDLLSTNKTDFFREPKHFDFLRERILPSLEKEKR
                     IRIWSSACSTGEEPYTIAMTLHEGVQQPAQWNFQILASDISTRVLAHAAKGLYAEDRV
                     REVPPEIVKHHFLQGRGDSAGLVKVKPHLSAIIKFRRLNLMDDHFPIKAPLDVIFCRN
                     VMIYFDRPTQERLVNKFYHHLKPGGHLFIGHSESLQWVSHPFKTIAPTIYWKES"
     gene            5428..6045
                     /locus_tag="PK589_02340"
     CDS             5428..6045
                     /locus_tag="PK589_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18461.1"
                     /translation="MAIIDTDAFSHIRRMRDGRFPHEVACILPGEFFVSREPMVVYTV
                     LGSCISACIRDPIVGVGGMNHFMLPAPKEHQSGDAWGGESTRYGSFAMEQLINEILKR
                     GGLRHRLEVKLFGAGKIYDGNIDVGARNTEWVLQYLKTEGYALAKSDLGDVYPRKVYY
                     FTDSGRVLMKKIERVKNRTIYERETEYQHRIEVEPTVQQDNITLF"
     gene            6107..7153
                     /locus_tag="PK589_02345"
     CDS             6107..7153
                     /locus_tag="PK589_02345"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNJ18462.1"
                     /translation="MAKIRVLTIDDSALMRQVLAELLSKDPGIEVIGSAPDPYVAREK
                     IKALNPDVLTLDVEMPKMDGLTFLEKLMRGRPTPVIMVSSLTEAGCQTTLRALELGAV
                     DFITKPKIDLREGMDQVAQDLIAKIKAAATASVRPTTAAASSTSRPTSLNSAMIKTTD
                     MIIAIGSSTGGTEAVKDVLQVLPPNTPPILITQHMPERFTKTWADRMNQLCRISVKEA
                     EDGDSVLPGHALVAPGNYHMTLVRSGARYSVRINQDEPVNRHRPSVDVMFDSVAQYAG
                     GNAVGVILTGMGGDGAKGLLRMKEAGAFTIAQDEASCVVFGMPKEAIKLGAADIVRPL
                     GDIASAILTQVTRA"
     gene            7185..7523
                     /locus_tag="PK589_02350"
     CDS             7185..7523
                     /locus_tag="PK589_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNJ18463.1"
                     /translation="MKITSEIHNHKVTLKLEGNFTYTQRKPFQEMLKSVSVDPMEHIV
                     IDLSQVAFLDSAALGLLMISHRQLQAEKRTLSLAYPQATVRQIIELANLHKTIPLIES
                     PASTVTKKSA"
     gene            7556..7906
                     /locus_tag="PK589_02355"
     CDS             7556..7906
                     /locus_tag="PK589_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNJ18464.1"
                     /translation="MQITERRVGESVILDLVGELTYANRATFKASVERSRAGGVRHLI
                     LNMQGVRFLDSSALGTLALLTQSLSATRGTIGLLNPQSYVKEILTLANLHQLLPVYHS
                     EQDALAACRLPKAG"
     gene            7930..9195
                     /locus_tag="PK589_02360"
     CDS             7930..9195
                     /locus_tag="PK589_02360"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNJ18465.1"
                     /translation="MAEPARRIDPQSGTDSDAKPAPVILLVDDDEITRTGMAGRLKRL
                     GYRVIEAGDGTAGLTAIRAQRPDLVILDWMMPGMDGPSVCEAIRADPELKSSQVVLMT
                     AHDRPEQIAEGLSRGADDFLSKAASKQEVLARVQASLRSSALVREIEHTRDDLDRSHR
                     LLSAKQAELESELQSAADFVRAQLPLPGMPATGVSMQWAYQPSLALGGDLFQVCSWGA
                     DALGLYILDASGHGVAAALRAVGLMSFLREDNLAKVVGSFDPGAIITEANRLFPLTQD
                     GEYFTLWVGRLDISSRMLSYATAGHSGAFLQAAGDGSRWLSMASLPLGFDPHSVFESM
                     HTPLLSLDRLYLLSDGIYEAPSPTGELWGRDRLQATLEAHRSHSLGQSIADTMATARQ
                     WLAGEIFPDDVALLGLEMLDTLLGQKERA"
     gene            9195..9554
                     /locus_tag="PK589_02365"
     CDS             9195..9554
                     /locus_tag="PK589_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNJ18466.1"
                     /translation="MDSQPVMDLAAALDRFDGDQDLFLTLAGLFVERSGQALAALQTA
                     LSAQDLPVLVKEAHKLKGSAMEFCARPAVAAAAHLEEAARKAAAQDIPALAEQTRAEV
                     ERLTAALAEIMQKGFPS"
     gene            9551..10450
                     /locus_tag="PK589_02370"
     CDS             9551..10450
                     /locus_tag="PK589_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ18467.1"
                     /translation="MIGHAASPTMLVVDASPETWAMALEEGKLRGISVITAPDPQVAL
                     AMIDMAAPDILMTDLFPADQAGVLLIRELRMRSPKTAILATGESGDAETILNVVRAGA
                     GDYLRKPLLVEELGLALDRALRQLPSTVENIAGVEQVDYRLVLGTNPDHVEACVTWLI
                     QQTALTLPEMQRLHLRTTLIELIVNAVEHGSLEILYQEKHAALSSDQFERLIEERRRH
                     PRFARRRVVVQASHDKTRRRLRYAITDEGKGFAWSRFVATPEQPCDSRHPNGRGVFLA
                     KAFFPDLTYNERGTEVTFSVPVP"
     gene            10557..10796
                     /locus_tag="PK589_02375"
     CDS             10557..10796
                     /locus_tag="PK589_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18468.1"
                     /translation="MTSLTRPVFLLMLCAFSAGCSTAEWVHPNRPKDQFTLDYNKCQA
                     DTMRDPKLQQGIQLLIIQATERCVQKQGWRLVEKE"
     gene            10991..12091
                     /gene="wecB"
                     /locus_tag="PK589_02380"
     CDS             10991..12091
                     /gene="wecB"
                     /locus_tag="PK589_02380"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNJ18469.1"
                     /translation="MTRIDLIAGARPNFMKIAPIIDALNAARSRGSHLRFRLIHTGQH
                     YDRAMSGSFFEELGIPDPDINLEVGSGTQAEQTSAIMVNYEKVLAKDKSHLCLVVGDV
                     TSTMACSIVARKMGIPVAHVEGGIRSNDWTMPEEINRVVTDSITNWFFTTSETANENL
                     RRAGVTSDRIFFVGNTMIDTLLKHADRLRPPACWSSLALKPNHYFVVTLHRPANVDGE
                     QQLLGLLRAIAEGTQGLPVVFPVHPRTAKNLRESGQALPSMHYVDPLGYLEFNYLVKH
                     ARGVITDSGGITEETTVLGVPCLTLRDNTERPETVTIGTNELIGTDPAKLPPALQRLM
                     AGQWKKGAIPPLWDGKTAVRIVEHLTTLLAKP"
     gene            12585..13796
                     /locus_tag="PK589_02385"
     CDS             12585..13796
                     /locus_tag="PK589_02385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ18470.1"
                     /translation="MSHVKSIALIQLHIPHYRVPLFAALQAEAARRGYALTVYSGEQQ
                     PMPFSHRKLSLRYLSHDKSGGCWMEGLAEAVAGSDIIIAPHGLQCLTIPFLWLQRNRL
                     CTSWIWWGHGYNFQASTQGSTAEWITSAIKNFLLTRGDGVITYTHGGAQYWCNRGLPP
                     ERVAPFLNTIDVEGLREARRQVTDDQLSLVRERLALGKKQVLLFSGRLYAEKQVDFLL
                     EAFSKIQQACPETALLILGEGQERARLEQLCSSLHLHDVHFLGEITDPHETSIYFKLA
                     ELLVIPGLVGLAIVHGFAFNLPLMTTERPFHSPEIEYLSSLNGRMTRHDTDAYAAEII
                     RVLSSPQEIEAMRWHCAESAQQLTLAASSSRFVQAVAQFSNPTGSLTSNRRRSQRTSF
                     SSSAATPSLRR"
     gene            13800..14963
                     /locus_tag="PK589_02390"
     CDS             13800..14963
                     /locus_tag="PK589_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNJ18471.1"
                     /translation="MLSRRQFLQISAACTASALCASLPRFAQAQETFESRVIFDESLS
                     WVTEHSAHSMCQRMKQAGFNVVMPCVWHGRGTSWPSNLAPWDSYKLDEMARWNSSFDP
                     LGKLVEIAAQYQIEVHPWFNVTLRQREFLPHFYGPGTPADAFDVHRPDFRTFISNLMI
                     ECVSRYPVHGINLDYIRSVSICQSPECARQYHHHTNRNLMLDRLSYGASADAREAIAD
                     WNEQAVGDIVRRVSSHLRKNQPNVLISLCGHPGHPNLYIQGQKSIKWADEGLIDVVYD
                     MRYPAEPNWEEIRSVQKTMKRPEAYAVLIGNYDLAGAHQISVSSSGARVAAQIVRSRQ
                     VAPGNGVGVYLYSLLNDEQITALRSGPFQTPASPKWSRAKGLDLSTPTGLLVQ"
     gene            15084..16418
                     /locus_tag="PK589_02395"
     CDS             15084..16418
                     /locus_tag="PK589_02395"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNJ18472.1"
                     /translation="MAYAFGFLILALLVKDTAPIGDQYVLRTLSQGLCLTAGFWWLFT
                     QGNIIAMRRYWLILLYSAVLCSTAFVSMDSTRVLLQVLSLVAVTLFFMSYCEQAQQMP
                     SMHDTAARIVLYALLPVCLGSLLLYKIAPTLAFDQTVERMVWDNPHRFKGLFGKPAGI
                     AAASGILLGLCAFTKVHWLIRAVGALSSLMCLYLTLSRSFWVGSSVALLVTAFLYAKR
                     KGLLLGAGIGGLLLILATVSIMDTKIPGLNQSRSMRSDSLENLSGRTTLWTLALDRFW
                     DRPVLGYGYTVGHQAFLYSRKAKGADVAGDNVDLFRNETFSLHNGYVQALLDSGGMGA
                     LLYTIILAITVWRIVWYDRVKAHAAALYSLVFLSIANIGETLVYTPATFHAAFYWYVA
                     VLALGLRFTVEPPPASFAEPIVLPVRERTTKYPIVSRPEPQVQQGKTSPGLC"
     gene            16564..18090
                     /locus_tag="PK589_02400"
     CDS             16564..18090
                     /locus_tag="PK589_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNJ18473.1"
                     /translation="MKKSDEHSHQGQTDQLVVAGSAVLLSSIFGNGLNYLFGVFLARF
                     LGADQFGLYALALAFFTTLTLVVPLGMDAGAIKFISEYRTRQDSEGAQRIVLMAGTVT
                     FLTAALTAICLAGLSSLISLSVYHKPELNILLLYVAAGIPLYALTSILLASLQSHHTV
                     RPLIVVRYLWEPIGKFILAGLVISAGWGLAGVFGALLIAMLVSLTLTLGFLRTVVRFR
                     RPMIDLWSREEAARLMTYCLPLGVATVFGVVAPRADILMLGSWVNVHEIGIYQAAFQT
                     AAALALILGALESSLTPFFGQLHAQNDIAGLRHMYQTASKLVLLFTVPLFVLLAVFSE
                     EVLSLFGTEFRAGGTLLIVLASGQLLSSAGGSPNNLLLMGGHSRLVMWNTIGVGLLAF
                     GVFACTIPLWGVLGAAIGAASTQVFGVGLRILQVWRIFHIQPFSQRLVKPLLAGLGSA
                     VLGLSLKPDAPALLLPGLGLMMGLVYIVLLIALKLDPQDRQALNALVSKAKMIVAVRA
                     "
     gene            18109..19287
                     /locus_tag="PK589_02405"
     CDS             18109..19287
                     /locus_tag="PK589_02405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ18474.1"
                     /translation="MKLAVLTNILTPYRIPLFQAMAEQVDEFSIILMAQQEENRLWKI
                     EDVPFPVYVLPGFHVRPWGAPVAVHLNYGVTARLTQLDPDVVLNAGFAVANIAAYWYC
                     RRAHIPFIHWAHLSLNDGAQASFVRRRIRHWLIGRSAGAVGESSHARDAFIHYGAPKE
                     RILVATMPLDVTGLHDRVLARKQWPETSTPLNTLSKPVLLSIGQLIPRKGYHELFAIY
                     ERLLTTHPHTSLLIVGDGPDRQHFETVVREKGWTRVHFAGFVQAQDLPRYFALSDAFI
                     FHTLYDPFGLVLSEAMAAEVPVVSSIHAMATHDLVEDGVTGFRIDPLQPEASAETVRH
                     LFDMSSDQRTSLIDAAYRRVLPCDSRTSASRIVEFLRSFRTPHEPRMATTPSPSQRIS
                     "
     gene            19284..20459
                     /locus_tag="PK589_02410"
     CDS             19284..20459
                     /locus_tag="PK589_02410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ18475.1"
                     /translation="MTTRNDTIEVIGPTPPPYHGVSVATQVLLDALPHSQLAVVHLDT
                     ADRRGIAHVDQPDLWDVFLFIRQWFAHLFILLRKRPRICYLPLSQSRVGFLRDSLFMV
                     PAFLTGSAVVAHLHGANFYQVYEGGGTAFKWYVDQVLKRVAKFVVLGDMLKPIFHRWA
                     SLESIAVVPNGVPELSTDGSPGPHCVRSAGPRIVFLSTLSRNKGLFVLLDAVPLVVQE
                     FPTAEFHIAGPWWGADTERLVRAQLEQGNVSSQVIFHGQLAGAKKMAFLRSGDLFVFP
                     GIQQEGQPLTVLEAMCAGLPVIATDRGCLRETVMEGETGFVVPPNSPTAIAERILLLL
                     REPHIRQRLGANARLRYEREYTMPLYAARMADLFAQVSQSDYHENPKSMDSRHWSIR"
     gene            20459..21328
                     /locus_tag="PK589_02415"
     CDS             20459..21328
                     /locus_tag="PK589_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ18476.1"
                     /translation="MNTLHDIEQFWDANPCGETLVGKDQEWATFFNRYDEFRYRTEGH
                     ILGELDRLQLAGKNVLEIGIGQAADSAQIIRRGGRWHGLDLTAEAVFRAKTRFELFQL
                     PYQAVKQGSATAIPYPDKSFDIVYSHGVLHHIPPIRQVSPEIRRVLKPGGKLVIMMYH
                     KHSLNYLVSINIVRRLLMIGLFGLAKIGARPLITHPLLAGHLQNAEEFGLWTYLQNPL
                     FMMRNTDGPGNPYSKVYDAQDIAADFAEFRLENTRVHFLNERHLPVLKLLPPRVRTYL
                     ASKLGWHLWATLG"
     gene            21363..23264
                     /gene="asnB"
                     /locus_tag="PK589_02420"
     CDS             21363..23264
                     /gene="asnB"
                     /locus_tag="PK589_02420"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNJ18477.1"
                     /translation="MCGIVGFMAQDAESALTERTLTIMRDQMIHRGPDDGGVYVRREG
                     ASTIGLGHRRLSIIDLSPLGHQPMSTADGSLWIVFNGEIFNYRELRAELPARIQAQFR
                     SQTDTEVILYAIREWGLDGCLQRLRGMYAFAVYDSTSEILTLVRDPLGVKPLYYTHDR
                     GRVTFASEIKALAAMPGFKPVINDQALYHYLTFANAPAPQTFFEGILKLEAGTYLTCD
                     KKGSMRQHRYWNPSTFQPGSASLTEQDYVDELRRLLRQSVRRRMVSDVPFGVFLSGGV
                     DSSLNVALMAELLDRPVETFSIGIKDDPSNEFDYARQVAEHFHTNHHEAVISDEDFIR
                     FLPQMAYLQDEPVADPVCVPIYYISKLARESGTPVIQVGEGSDEIFAGYQTYHLFDDW
                     NRRYYEPYLQAPEFVRRAVYSLGSHVCSSEKQDALRRAMLGDPLFLGNAIAFWDNEKA
                     GLLHQAFLPRHTSSEYIRNLGGSLGIDDSLLKIIHVELKNRLPELLLMRVDKMSMANS
                     IETRVPFLDEDVVSFALTIPSALKYKQNQPKYILRQAARGIIPDSIIDRKKWGFCGSA
                     TNMLSTRLTDYARSRILDSPLMHERFDRNAIEVIFIALRQQKRFNSFKIWNLLNLVLW
                     YERWFASTR"
     gene            23549..24238
                     /locus_tag="PK589_02425"
     CDS             23549..24238
                     /locus_tag="PK589_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18478.1"
                     /translation="MMKRALNLSLSLLVLAVGSVLPIGEETRAEAALVTYEFSGNISE
                     VPGSLFTSGGTGANGFNTGLKLSGTYTFDTAAAGLLPSSLPIQLYNNSVSSLNLSIGN
                     YSAVLQGGGTNLTTVSSGNPAFYSVNVGTAGNLVKGLAPSLFSINLADETGTAFSSVT
                     LPGNQAPNLNAFNVNRWTLAFSNGKTLVGSLDTLKAVPLPATVLLFGAGLIGLVGLGA
                     RGRFNQSSVRG"
     gene            24533..26692
                     /locus_tag="PK589_02430"
     CDS             24533..26692
                     /locus_tag="PK589_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HNJ18479.1"
                     /translation="MKQVIQNFRSGVLKIDDVPPPMLRDGGLLVMNHVSLISPGTEKS
                     TVQVAQKSLLGKAMERPEMVKKVLTAIQKDGLKDTLQRVFERLDTPAALGYSCAGTVA
                     AVGADASQFSVGDRVACAGQNYASHAEVVYVPKNLCVKLPTTVDFEDAACVTLGAIAL
                     QGVRQAEPRLGDRIAVIGLGLLGQLTVQMLKAAGCRVLGSDLDGTKLDLARQLGADLT
                     VRPSGLMDAATGFSEGHGVDAVIITASTKDNSPVEIAGAIARKKGRVVVVGAVGMNLP
                     REPYYKKELDFKLSMSYGPGRYDSAYEEKGQDYPYSYVRWTENRNMAAFLDLVGDGKV
                     QLKPLITHRFEISKADDAYRMMTEGQTPYMGMLITYPLDQQQALQPSITVVSDKPTGR
                     LTLGLIGVGNHVKDMLLPQLQSQAAVSIRAVCTASGINAKALGEKLHAGYCTSDSQDV
                     LRDPAINTVLIGTRHDTHGRLVVKALEADKHVFVEKPLCLTEEELDAIHAVYCDKAAK
                     GLRLMVGFNRRFSPHAEKAREFFAHRENPLVMWYRINAGKIPAEHWIQDPSVGGGRLI
                     GEACHFVDYMQSVCGARPNSVFARRIGRQSSGITDDQCIITLTFEDGSIGSIIYTAGG
                     NSGLAKERFEAHADGKSLTMDDFEETHLYSGSHREVFKTSKRDKGFKTEIAHFVQTVE
                     QGIPCVMSFGEIAAVTRACLLAVRSLQSGSAHDIAYV"
     gene            26685..28628
                     /locus_tag="PK589_02435"
     CDS             26685..28628
                     /locus_tag="PK589_02435"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNJ18480.1"
                     /translation="MFSIDFSVNTKVLPKGLAWYAKRLSVMGPGEIMHRMREGIAMQS
                     LQLQYRLSKRATAQHYGPKDIPQHVFCSGQDQRLPSLPWKFHLAQEEIACLLNGTLSA
                     LSHEWTWRADAKVWHEAPDTRQLWPQIFFHRIPYREGNPFGDIRIAWEPSRLQHLLTL
                     ALYAQQAEPDKRRQAVAMIEGQLLSWFAANPPLTGIHYISVMECALRLMAVCHTLDLV
                     RPWLSQPEAIWAGTLNLVSTHAELIRKRVSGHSSSGNHTIAEAAGLVYAGLLFPKMEM
                     AERWLAYGLYLLEQEAPHQIRQDGGGREEAIWYLRFISDLYGLTVALLRQHEQLIPQK
                     LIQATERSHTFLQAMMRPGDGALPRIGDADSGYALSPFLQFVPVSAEAAPGLTSFHLS
                     GCSILQGSKGERLLFDHGELGMAPCYAHGHADALSVQLEIGQQEILIDPGTYSYTGHS
                     EWRRYFRSTRAHNTVTVDGRDQATQETSFLWSQPFETHLIYRDETPEGKITVLARHYG
                     YTQPCGVTHWRGVVYQPPGAWLIWDWLTGTGTHHLELNWHLGVSPVQNSASYVLACRE
                     QPVQISVEGGLASLHHGETAPPSGWRSSTYGSKEPITTLRAEYRGALPHEFLTRIWCG
                     EGPPPTQLQTFSLSRSFHGSNTR"
     gene            28609..29343
                     /locus_tag="PK589_02440"
     CDS             28609..29343
                     /locus_tag="PK589_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNJ18481.1"
                     /translation="MVPTRVDILNIPVDCVTMDQAVAWADSRMTLSEPKTVIAVNPEK
                     IMKAQSDPTLRQFLRSAGLLIPDGIGVVIATKLLGLGYAERVPGSELMPRLCGLAAEK
                     GYSIFLFGASPEVNERAAHKLRQEFPGLTIAGTQHGYVDDADMPAVIDHINAVAPHFL
                     FIALGSPKQELWMSTYLPRLRVKVCQGVGGTFDVLAGRVKRAPWIFRCIHLEWFYRLL
                     SQPSRLVRQTALPLFAFHILRKRVLG"
     gene            29340..30959
                     /gene="xrtD"
                     /locus_tag="PK589_02445"
     CDS             29340..30959
                     /gene="xrtD"
                     /locus_tag="PK589_02445"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM-specific exosortase XrtD"
                     /protein_id="HNJ18482.1"
                     /translation="MRSTPELATKTARFLAEPVASVSAAAPTRSLALGLTVLLPVTIL
                     GLVFTDSVAYMADLWLTDENYGHGMLVPLISLYLIWQQRRRLMEQSRSGSWWGLIWIL
                     LGAALYVVGELATVYQFLHVALWCIIVGLLVSALGARAVSTIAFPLGFLLTVVPLPQF
                     LYQGLSGKLQLISSALGVGCLQFIGITAFRDGNVIDLGPIQLQVVDACSGLRYLFPLA
                     TLALLCSYLFRERLWKRIVLFISSIPISILLNGFRIGMIGVLVEHFGQGAAEGFYHAF
                     EGWVLFMASLGLLLAEMFVLRKIGATRVTRDTPSQPQPEMVPPASLPSRAILLRPTYL
                     YSVGLVCLLPFAATELTERGDVPPVRQPFLDFPMQFKGWSGTPFPLEGRYVEVLKFDD
                     YVLADYQQPQSAAVNLYIAYYRSQRKGQSAHSPQSCIPGGGWEIGTIETVQLDSAAGQ
                     APANRVSIQKGDQRQLVLYWFKQRHRIVANEYLVKLYVLWDAMTRQRTDGALIRLTTP
                     IQQNESEGAAEQRLLHFAQDMNPILTRYVPD"
     gene            30979..32607
                     /locus_tag="PK589_02450"
     CDS             30979..32607
                     /locus_tag="PK589_02450"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNJ18483.1"
                     /translation="MISTLFFSFVGSLIICMALIPPLMATASRLQFLDIPKDQRRVHA
                     IPVAKVGGIAFGVGTFSAMLIWAPKDDIVVAGLLGGVTILLFGMWDDRVGLPYTIKFL
                     GQTLGALIIMWQGNIHLNSLPFVETELPLWVAYPLTTLIIVGVTNAVNLADGLDGLAG
                     GLSLLSFAGMACLAYLGGDQTVTLLMVPVLGGLLGFLRFNTYPARIFMGDAGSQFLGF
                     FMAVSAIVLTDPQRGPYTPALGLFLLGLPLLDTVGVMLQRLKEGRSPFVADKNHLHHK
                     LLAMGLSHYEAVLMIYLLQAALVTLAYILRWQSELAILSTYGLVATMILYPFLRSRSW
                     KIQSNRMLKTADRLLTRASTITSSPWLTTEPIQILGIAVPAFLILSVFLPKHVPDDMG
                     YVAAVMFAVMLIGLWASPRISSLVVRGGLYVGSTFIMYLSEQPASSTDILSPIHIFFV
                     GLAVLVALTIRFNSEHRFQTTPLDYLMVFLAIMVPALPEMQMGDIHLSFLTAKLIVLF
                     FSFELLLHVFARRLTQLALVALWLLCGIAVRAWI"
     gene            32625..35030
                     /locus_tag="PK589_02455"
     CDS             32625..35030
                     /locus_tag="PK589_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ18484.1"
                     /translation="MATTWHRCHTIARFGLAIVLILAVGCGGPEERKAKYRTKAQEYL
                     QAGNFPKARVALRNVLKIDPKDSEAYFLYAQVEEKEKNWRNAFAHYQRVIELEPDHER
                     ALVKLGKFYLQGRLDEKAHETADHLLRVYPGHVSAQAIKAALLAADGKLTQAVVAGEA
                     LFKQFPTEPDAAILLSTLYALQNSPQRVEAVLRKAVAAHPHDLDLLNSLAMAYMRAGN
                     QAQAESVFRDILALEPSVFDHHLKLALFFDQQRQYDKAEAILREALRLDQDNEQRHLA
                     LADYLAGRKNVETAEAALNEAHRVLPHAVNIQFALGRLYELHQLPDKARATYEDIRDH
                     NRSKPAGGNAKIKLATLDWAAGKQDDAMQQLREVLRDNPRASDALMLEGRIALQHGSA
                     REAVQAFRTVLKDQPEQSEAYALLGQAHLMLEETSLARENLERAVTLSPNLYQAHLAL
                     AILDASSGRTRDARVRLEELLKQAPTNLGTLGMLLNLQAADRDWDGSDQTLSRIREAG
                     ANRFVADLAEGNLYQARLQWDKAVAAFERASSLNPDAPEPIFALVRIDARLGKLAQAH
                     NRLSGLIAKNAQHPFAHGLLGEILILKGDPMRAEQEFRLATTLKPEWGTPWVNWVTLK
                     LSQRKHEEAGDILQRGLQINPASEELRLLYASHLGERGQFDQAILEYETILKHNPRQL
                     MAANNLASILADQKGDAKSLDRALALSREFEQRAPNPFFLDTLGWVHLKMGHQDDAIR
                     VIQRAVTEAPQHPILNYHLGMAHFKAGHRVEAQTHLKKALNTKQPFPGMDEAKSVLAE
                     VTG"
     gene            35042..35659
                     /locus_tag="PK589_02460"
     CDS             35042..35659
                     /locus_tag="PK589_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNJ18485.1"
                     /translation="MKIAGSLLIKKLARGCQPGLLALYAVVLMTGCVTPEHTAAQNMA
                     DPGYHLGPEDVVMISVWKDENLTREAIVRPDGMVSFPLIGDVPAMGHTVEEVRQDIAK
                     RLARFIPNPHVSVAITKLQSYKVYVMGRVTKPGEYLVGHYTDVLQALSLAGGLTPFAS
                     ENDIKIIRRENGDQRVFLFRYGDVQKGRDLNQNILLERGDVVMVP"
     gene            35700..36905
                     /locus_tag="PK589_02465"
     CDS             35700..36905
                     /locus_tag="PK589_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18486.1"
                     /translation="MKHRLIPTAWLWAILLTTAWSSHSLDQEVWSAEWFLEPALTVRG
                     EVNSNLTLTTFDHKTTYGHWVSPGAKFGGSTEALEVNGRLAADFVRYYGGQNTSLTNL
                     YFPLSAKYKMERDSFGFDGGFTRDNTLMGELRQTGVVLSFTQRNQWILNPSWTHAITE
                     RLSATGSYTFYDTRYENGLRLGLADYTLHVGSTGMTYQVGERDQVQLNAVGVRFNAAD
                     LRLSSTIIGPQITYSHMFSETLTGTVSGGPRHVSSTINTSAGSLSDGVWTWVFSGNLE
                     RRWEDWRVKGEIGREINPSGFGLLLRTDKLSLEVSHQMTDRLSASVTGLFLIADGVKT
                     RAVSTVFPENRLFYVTPKLSWRLSEWWTADITYTYAQRDLPSQSLTALGNSATIMFTY
                     YPPRFTVGR"
     gene            36940..38685
                     /locus_tag="PK589_02470"
     CDS             36940..38685
                     /locus_tag="PK589_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNJ18487.1"
                     /translation="MNKPTVGIQDIISMAKRRKMPIIMITLGILLLGATLAFTIPAVY
                     RSTATILIEEQEIPSDLVRSAITTYADQRIETIKQQVMSRSTLWKIVEQYDLYKHMRK
                     QSPTEEVLHRFTKDTNIDVINAKVVDKRTQNPTQATIAFTLSYDGESPESAQKVANEL
                     TSLFLAENLKTRERHAQETTAFLKQEADGLSRHIEEIEKKLSIVKQRADGALPELTQL
                     NMQLLNGAERELTDTDRDIRSLEERKTFLEGELATIKPNTPILSSTGERILDSGERLK
                     ALRAEYADKAGYLSPEHPDIWKMQQEIRALERETGGAAPIDDVLKRLTGERATLATLL
                     DKYGEDHPDVTRSRRTISSLEKDLREYENAPAKMPAIKPENPAYINTQTQLASATSML
                     EALKAQRAKLRKRAEDYARRLEKTHHFEPEYLDLARERDNSARKYEELRSKLLEAQVS
                     EGLETQRKGERFSLIDPPDLPEKPEKPNRPLILLLSFVLALAGGAGSGVLLENVDRSI
                     RGPESLAHITQTYPLAVIPYIPNNADLRQLIAKRRTIRLAGIGIAAACILLAHFFWLP
                     LDVVWFAALRKFGLD"
     gene            38704..39540
                     /locus_tag="PK589_02475"
     CDS             38704..39540
                     /locus_tag="PK589_02475"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HNJ18488.1"
                     /translation="MEPLHSAMERFKREEQRRPTPVVLKAPPPRIGKDAVQDIVYTHT
                     RTVDIPDDVLRERRVLAGFERGPFLDAYKILRTQVMHRLREHGWNVLGITSPSKQEGK
                     TLTAVNLAISLAMDTTQTVLLVDADLTNPSAHLAFGIEECGGLVDYLLDGIPVEHLLI
                     HPGIGRFVLMPAGRSITNSTEALTSPKMASLVEEFKHRYPSRIVLFDLPPLLQTADVL
                     AFSPYTDALLLVVEDGKTTREEVERALQLVKGSTPVLGTVLNKAGKGHMTPAAMKRML
                     LT"
     gene            39567..40991
                     /locus_tag="PK589_02480"
     CDS             39567..40991
                     /locus_tag="PK589_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ18489.1"
                     /translation="MYERFYHLTVKPFTLVPNPDFLYRGETHRLALTTLEYGILNHAG
                     FVVLTGEPGMGKTTLIQQILAEHRNHFTTGLITNTHEGMETLLPWILLAFGQNDKNLD
                     KLEGFHAFQQFLGKEAAAKRRVLLIVDEAQNLGANLLEELRLLSNLNKDKAPILQIIL
                     SGQPDLRQLLQRKDLTQFAQRVMVDYSLEPFKEEDSIGYIAHRLRVAGREEPLFTHEA
                     ARTIHRLTGGIPRLINQLCDTALTYGFAEEAPFLSARIVAQAAKDRTKGGILPLSNTE
                     SLTAVTPDQQAAEQTELAALSARAASTSNEVPRRDDAVPTSSGVATARPDTNEQYERG
                     LSLKKAGSYKEALQAFSLAAQDAAFTFKAMAQVGICLKSLAQPEEAVTAFRKSLQAKR
                     SASPDTIQVRYLLARTLESLERTDDALEQYRWIGREEPSFKDVAERLERLHGRPIRST
                     RNEAASSDGSSWVTQLQRILGASK"
     gene            41081..41803
                     /locus_tag="PK589_02485"
     CDS             41081..41803
                     /locus_tag="PK589_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ18490.1"
                     /translation="MALEIIEIVEDDQSQAKLLDQILRQASYRTNVAFDGPAGMQDVW
                     RIKPSLIVADDNLPGLTGREMCKRLRQDPSTTHIPIIVMSGFSSEERRAEALDAGADD
                     FIVKPYSGMELLARVRALLRRCRQSQAQDEELPEDLALTENLYVAVYRGKSLTLSAHE
                     WKALRRLASTVGNVVPREELKSLLWGDDSLMHDGELDRCMQQLNRKLSQDAPSVESIK
                     IVPGGFRLSTPASDDSGGPAVH"
     gene            complement(41780..42634)
                     /locus_tag="PK589_02490"
     CDS             complement(41780..42634)
                     /locus_tag="PK589_02490"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNJ18491.1"
                     /translation="MGDMLHSIHTGPQSPTQGLLTMAASSHATVLHLLRQAIAGREHV
                     DTIHAIRTHCRRLQALLELYGEAKRARAVAACVRRLSKLRALQVFRQYVIRCHAGRKD
                     VAVVDDRLTKQIRKLNHTEAYRRIEQVVSKQALPAILSPPQSWNRRLDELRQEHECAL
                     KRLIAAASDKPRRKRLHALRLALKTIRYQTEWLPGRTAAKEEMLSQLKRVQSLLGKYE
                     ELADFHRWGKKLSLPVRKRMKKDWKRARKRARHVPGTMSWLLDALASGHVWSGENLHV
                     ASVNRRSA"
     gene            42738..43235
                     /locus_tag="PK589_02495"
     CDS             42738..43235
                     /locus_tag="PK589_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNJ18492.1"
                     /translation="MDCLLFRHGIAYDREDWRGNEHNRPLTDKGLVRTRQSSKGLLTL
                     GCIPTLILSSPLTRAQQTAKIARSLIPATPPLRICQELNPEVPPRALIALLNTLPSDA
                     VVLCVGHEPHLSATAGILLTGKLCKGLSLKKAGACLLHLEGSVRPAKGRLEWWLTASQ
                     LRALA"
     gene            complement(43361..44914)
                     /locus_tag="PK589_02500"
     CDS             complement(43361..44914)
                     /locus_tag="PK589_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNJ18493.1"
                     /translation="MPKLAVLDIGTNSIHMVLAEVQPDYSYKILDRFKDMTRLGDGVF
                     TSRRLSDQAMMRGLEVIRTLVNLARNKGYEQIEGVATSAVREARNGGEFLDHVMQQTG
                     LTVRVITGAEEARLIFLGVQNSVALPESPTLVIDIGGGSVEVIVGNREAIFQARSLKL
                     GAIRLKDLYLSKTPPSKAMLRDLEAAVTAQLKNALGSYKTKRVEQIIATSGMAGNLAE
                     VIHLQRTGRPLPQLNLARVSAKDIAAVEKRLAGASLKTRLAMPGLDPKRVDTLLPAAI
                     VFRILLELLQKTELTLCDKAIREGIIYDFIQRHHDRIQAERDIPDVRKRNILALAHRC
                     HVSETHALHVAGLALRLFDQTKALHGLGQREREWLEGAAILHDIGYMINSRQHHKHAY
                     YLIKNSDLSGFTAEEIDLIANIARYHRRSVPTRKHDEFTVLPEGSQRVINVLSALLRI
                     ADGLDRSQFSVVQNIDVKLGKTVVITAQVSGDAELEIWAARGRSDLFEKVFKRPVQFI
                     TTTRDDDAT"
     gene            complement(44915..45583)
                     /gene="phoU"
                     /locus_tag="PK589_02505"
     CDS             complement(44915..45583)
                     /gene="phoU"
                     /locus_tag="PK589_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNJ18494.1"
                     /translation="MQRHFDQDLAHLKQQLLRMGGLVESQIQQALQALVDRDSDLAVD
                     VIKQDHDVNGLDVEIDELCVQLLALQQPTARDLRFVTTGMKISSELERMGDLADNIAQ
                     RALELNSEPQLKPYIDIPRMANWTMRMVKECLDAFVNSDPVLARKVCTDDDFVDEVNE
                     QLFRELLSFMLEDTRTITRAIRLTFVAKSLERIADHATNIAELVVYMVEGKNIRHVAP
                     TASF"
     gene            complement(45605..46360)
                     /gene="pstB"
                     /locus_tag="PK589_02510"
     CDS             complement(45605..46360)
                     /gene="pstB"
                     /locus_tag="PK589_02510"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNJ18495.1"
                     /translation="MQTKISVRDLDFYYGHRQALFHVGLTIRSHSVTAFIGPSGCGKS
                     TLLRCLNRMNDLVEGARPVGRVELDGMDIYDPAIDVTDLRKRVGMVFQKSNPFPKSIY
                     DNVAYGPRLHGIKDKRSLDERVQHSLQGAGLWDEVKDRLQHSAVGLSGGQQQRLCIAR
                     ALAVQPEVILMDEPCSALDPIATGKIEELIYSLKETYTVVIVTHNMQQAARVSDQCGF
                     FLMGELVEFGDTKTIFTTPRDKRTEDYITGRFG"
     gene            complement(46487..48136)
                     /gene="pstA"
                     /locus_tag="PK589_02515"
     CDS             complement(46487..48136)
                     /gene="pstA"
                     /locus_tag="PK589_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNJ18496.1"
                     /translation="MGKPSLRMKEFWRGGELFVWMTASGVAISLLMVAGMLVLIMFNG
                     LGQFWPGTLQQVTLKTQETVIGHLVGEETIPHSMTTDALLGQRRFRYRVGNRDQFGSE
                     YRWINESEIASISAPDDLVFVERREWGPAYGRISLAAESTAATNADGSAWSTIETLVD
                     RANQLRQRIEHLEREDIGSINYRIEKARLARQTLVLKGAHNPASIEADRRLVEQIAAL
                     EREYLSKTDALEQLHKEATQEVVLLTLSTGRSVRLSLDQVLAVSRPNAMNGFEKAVAY
                     VRNLWHFVSGEPREANTEGGIFPAIFGTVLMVFLMTLAVMPFGVMAAVYLREYAKQGP
                     LVRLVRIAVNNLAGVPSIVFGVFGLAFFVYALGGTIDQILFPESLPNPTFGTGGILWA
                     SLTLALLTVPVVIVATEEGLSAVPRDFREGSVGLGATKFETIRHVILPCALPGILTGL
                     ILAMARAAGEVAPLMLTGVVKLAPALPLDEYLPFLHLERKFMHLGFHIYDVGFQSPNV
                     EAAKPMVYMTTLILILVVVLLNLAAVMLRNRLRKRFATSAV"
     gene            complement(48129..50357)
                     /locus_tag="PK589_02520"
     CDS             complement(48129..50357)
                     /locus_tag="PK589_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNJ18497.1"
                     /translation="MITNPLSYLSGPVHLRSVIDRLARGIITVGGLATIVSILGIFFF
                     LFREVTPLFTAPSAKLSQRLSVPALLADEGPAQVAVDEHREIAQVFTSGAIQFFDLAS
                     GQPIALEMPAQLTSRQVTAMASGGGPTARLAVGTAAGEILLLKIGTTTEFSDQGERRK
                     RPHVRAGAPIPVTDSPIVRLAYRSNDHGSLLALATKTGRLFLARIAPDDPTGAGVIVT
                     ELPQPPGGVASLALEASLEHLYVGTTDGHIIHVGVSETQPPDVRASYAVAGPTTAISM
                     LGFLSGDRSLVVMAADGAVSTWGLVRRSDVPTGWTLTQMHTFRSHAAPVTAFAPSQRV
                     KGFVTGDTKGNVFLHHATSSQTLLRVANGEFPIRAVAFAPKGDGVIALDGAGQLSLYQ
                     VQNPYPEVTLDTLFGKVWYEGYGQPAYVWQSSSGSDEVEPKLGLLPLAFGTLKGTIYA
                     LFLAVPIAIFAAICTSQFMHHDLRAKVKPIIEVMAALPTVVLGFLAGLWLAPLLERML
                     PALAGMVLVLPVLVIVASFSWHLCPMSIKRHFSPGNEALLLMPILALGIGGCLWAHSF
                     FEVWWFDGNIKTWLSSRLGIQYDQRNALVVGIVMGFAIVPVIYSIAEEALSNVPKSQI
                     AGSLALGATRWQTVLHLVLLSASPGIFSGVMIGFGRAIGETMIVLMATGNTPILDWNW
                     ANGFRTLSANIAVEIPEAPHGGSLYRVLFLAALLLFIVTFVINSLAELVRQRLRDKYS
                     HG"
     gene            complement(50357..51367)
                     /locus_tag="PK589_02525"
     CDS             complement(50357..51367)
                     /locus_tag="PK589_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNJ18498.1"
                     /translation="MTVSRQICLSLTLTALGPFMTMCLVSAAPGDTPAVETLTVDAGI
                     KPYHKVSGVSGTINSIGSDTLNNLITLWAEGFRKQYPNVKIQVEGKGSSTAPPALIEN
                     TAQLGPMSRTMKSSEIDAFESKFGYPPTAYPVAVDALALFVNKDNPVAGLTIAEVDAL
                     FSKSRRQGHTTDLTRWGQLGQNGDWANAPVSLYGRNSASGTYGFFKDHALKNGDFKDQ
                     VKEQPGSASVVQGISEDRYGIGYSGIGYSTSGVKMVPLAAKAGQPYIEPTQANTKNGT
                     YPLWRYLYIYVNQAPGKPLSPIVKEFLAYVYSKEGQSDVLRDGYFPVDAKLAEQQLGK
                     IR"
     gene            51582..52274
                     /locus_tag="PK589_02530"
     CDS             51582..52274
                     /locus_tag="PK589_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ18499.1"
                     /translation="MRPSTVHIVEDEPLHAGLLDRALRLAGFDTLLSSDGASGWQEIQ
                     RRLPTLILLDLMLPALSGQEICRMVRQHSATRHIPIIMLTAVGGEPERIAGLDMGADD
                     YVVKPFSPREVVSRVRTVLRRGAHSADGVAPIVNAAVRMQGPYFAVLLGERELLLSRK
                     ETALLQTLLGRENALVAAEELLTLPEGERPGLLLQELDYDVRGLRRKLENSGAGSVDM
                     LPGFRYRFRSRL"
     gene            complement(52311..52646)
                     /locus_tag="PK589_02535"
     CDS             complement(52311..52646)
                     /locus_tag="PK589_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18500.1"
                     /translation="MLHSQLHVIQQSAGCQGQRVEGLGTFQPGGSTMVHQSAVLSSEC
                     RVLELLHGRKSLTLEQVVTLLPELSWNQVFKTIDELSRHGKIILLRRGFDYEIERISS
                     RQPALSCFG"
     gene            complement(52711..53310)
                     /locus_tag="PK589_02540"
     CDS             complement(52711..53310)
                     /locus_tag="PK589_02540"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNJ18501.1"
                     /translation="MKVMVHGDPGTDLSSTLLNHDRVIHQEVSQMTFTSFPVRVPRVA
                     DLAGRAIETDLTDLWMKGIKAVLSLLILTILIALTGGVIKTFLDIKLLLEAPVEVGLR
                     QIIVDTLILLAVVEVFKTTLTYFSEGRVKVTFIVDTILVVMLTEIISLWFKEADQTKL
                     LSLGAIVLALGAVRVVAVRCSPAQGDGNGKCAGSCREGI"
     gene            53482..54192
                     /locus_tag="PK589_02545"
     CDS             53482..54192
                     /locus_tag="PK589_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ18502.1"
                     /translation="MQHATEPLRHHGIPVLLVTKDEEFAMSVHQLLRPNGYHVSLAPT
                     AEAALSLASGASFKLALIDRRHKVVGTLRQNLAFRHIPMIALQPLGEPCTEEETVEDL
                     AAGFDIVIDSHRHREWLAIIKALLRRQEIQAAPLREFRVNTLCMNLDRHEVRVEGKSI
                     QLTPKEFQILRVLLQHPGRVLTRQELLNLVWGEDYALEEHALDVHIHALRHKLETKPS
                     APQWILTVRGVGYKLSTD"
     gene            54347..55885
                     /locus_tag="PK589_02550"
     CDS             54347..55885
                     /locus_tag="PK589_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18503.1"
                     /translation="MEMRPLVASISGLVGSAMLGLCVTASWADDSSRQGQSSHTVVAQ
                     AMGPAPNGTSSPPPAPDMPLGGTSIEDLLMQKGTITKEEWIQIRAEQEYKAGERDKRV
                     DSLDEWKSKAELLPILRDKVNFGLNALQWLYTHQDAHVPEGRSQDNLSIRRSEILFWG
                     RISDTLPRWHTLMEFQSINLLNNTPTGGSAATGGVPTSSTFFREAYLDIRPVQSWAPN
                     VNYFRMGIFRMPFGIFTETSGGLRDVISSPYLTSVGSGVGNRTGSAGTVEFLQERDYF
                     LDMRGKLFNRLEYVVGIMNNNNYEANATGSNAPKSFYSRVRLFGSDVSFISFTTIQGE
                     SNNANTNINGRGKGAFDRYGLDFRYTSKILPGFMVQGEIWQGHDGANQTTVGRPANGA
                     CDVQAICGGSGAPGVQRRTWYVLAKYLITDGPFENFEPVIMYEQFDPNTRIGNDLYTR
                     TIVGLSYYFENFPPKVQSKLQVNYEFRHHSGNGPNNVVDPTRGVGDPFAQNAFLVQWQ
                     IRFM"
     gene            55954..56493
                     /locus_tag="PK589_02555"
     CDS             55954..56493
                     /locus_tag="PK589_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNJ18504.1"
                     /translation="MKIYRGAIAAVLIGLVLNGCGPQRVFSPQVLDGVDPNFDFTAWR
                     MVPNAKAGKKVQLGGRILQAPQKDGLVTMVLTQLPIVEHPAYGPTDTERGRGEFLVYF
                     NGKVPTNHLQPGNRVIAIGTTQNAAVVAIEDSQRSLPALTAQCLHIWNTGGKEIADFP
                     NFGAGYQPLEEETYCAESR"
     gene            56828..57802
                     /locus_tag="PK589_02560"
     CDS             56828..57802
                     /locus_tag="PK589_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18505.1"
                     /translation="MPVASMVTIPYPRLALRRSLCLVLLMYLTSACSLLTGGKVLFDQ
                     QDSQVGVQTDPTVHRGPAPTENNHPAQLTTEEVRVLLGSLMVSGWSGTLVGILERPRP
                     IPVFTELELNRIAAPVAAAFQQATPSERVFFSLPKSQTTYSEERTTGALFLRGRYLHV
                     VLTDHSAFTRADTAGGEVKDLRDTKGMKLWVTAPATAATVPDAEEPRWAHFETVHVSL
                     NAKEVIAARNRPASPSADVSPSISLHPSEAVGAGAKSNGPADTTGNQTDELRLQVREL
                     TTSNLELRNRLDTQLKQMQALQDQLTKLQQDLDSARAKSKTPRRSTTP"
     gene            complement(58316..59434)
                     /locus_tag="PK589_02565"
     CDS             complement(58316..59434)
                     /locus_tag="PK589_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNJ18506.1"
                     /translation="MLSHGQKHGWRRRAVVVGLAGILATAESLAVLPAGVARAENAGP
                     TASLAIQEGLVQYNPQTQVAGSLRVQGSDTMSLLMSRLAGEFQRRQPNVSINVSGGGS
                     TKAVNEFVQPPAKLSGKITLKEERPTYVSLVASSRELLDAEVKQFVAQHGYEPTAIPV
                     AVDAVALYVHRDNPIQGLTLDQVDAMFSTTHNRGLKTSLSQWGALGLQDGWEKAPVRL
                     YGRDRRSGTRAFFQEHCLAGGEFAPSVREEPGAASVILDLTRDQLGIGYSGLGLETSN
                     VRIVPLAENQGMPFIAPTATTVSDQTYPLRRMLYLYVDKSPKAALAPAVQEFLTFVTS
                     REGQEAVMKAGFFPLPMNQLKKNFLALGLSGQETPLPN"
     gene            complement(59939..60322)
                     /locus_tag="PK589_02570"
     CDS             complement(59939..60322)
                     /locus_tag="PK589_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ18507.1"
                     /translation="MVTVSELMTKKLVTVPVGTTTAEAAKVMNERHVGSVFIEQNNRV
                     VGIVTESDIVRKVVGENKPVHFVPVESIMSSPVISLDERRSITEAADLMQCHHTRHLG
                     VLQSGAIVGVLSVRDLLQPVAVDEF"
     gene            complement(60529..61104)
                     /locus_tag="PK589_02575"
     CDS             complement(60529..61104)
                     /locus_tag="PK589_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ18508.1"
                     /translation="MIVLRPFALVGLLAVAAPFLFDGCASKAGTSGGATVTPSKPRVE
                     ERIAAAPVQEIPPAPEPAPVPLRSVEMAARNATGEQNIPFPDVLFDFDQYVLRDDALN
                     AVEANAKRLKDNRVTKVLLEGRCDEIGTAEYNLVLGERRALSVKRYLESLGMSQVQVD
                     VTSYGKDRPLCLQHNPVCWQKNRSVHFVVKE"
     gene            complement(61179..64868)
                     /locus_tag="PK589_02580"
     CDS             complement(61179..64868)
                     /locus_tag="PK589_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ18509.1"
                     /translation="MTDHHANRPANRLIRETSPYLLQHAYNPVDWYPWGPEALAQAAA
                     LKRPILLSIGYSSCHWCHVMERESFENEAIAALMNQHFICVKVDREERPDLDEIYMQA
                     TLALNRNQGGWPMTVFLTPDQKPFFAGTYFPPTDRYGRPGFPTLLKKIAEYWEKDHDG
                     VVAQAVNLTARLQEGAHAPSPTTVGEAELDMAVTQFAEDFDARQGGFGGAPKFPPATG
                     LSLLLHCYQRTKDPHTLTMVRKTLDAMAAGGIYDHIGGGFARYSTDERWLVPHFEKML
                     YDNALLARVYVEAYQVTADETYRRVACETLDYILKEMTSPEGGFYSATDADSEGVEGK
                     FFVWTPEDVRAAVDSDEEARRFCAYYDVTAGGNWEHTNVLHTAKSVEAVAKELSITAG
                     ELTETITRLRPKLYAVRAKRIAPGLDDKVITAWNGMMISAMAEAGRVFDIPRYREAAE
                     RACDFLLTTLSTADGRLLRTYRAGTAHLNAYLEDYAYFAEGLIDTYEAGGEERYLLAS
                     VRLAERMLADFADSQHGGFFTTATGHEALIMRSREGPDGATPSGNAVAASVLARLSSH
                     FAREDFREAATAAVRAYGRQIARYPRAFAKSLIVVDFLMSGPAEIALVGRLGDQGMES
                     LRAAVNRTYLPNRVLVHRDSDQSATSHPLLQGKTLIDGKAAVYVCRNFACGRPITDPK
                     EFPSLLDPRQQASSPPASEQKILSGTLYPGHATVQGTAAYAARHIHESNGALAHGFAL
                     FGATGLTASRLGFGTYRVGQREAEHREALRSALRQGCNVIDTSTNYMDGESEQLVGSV
                     LREMMRAGDLTREGVIVVSKIGYVQGQNLTQAQAREKSGKPYPDMVKYGDDIWHCIHP
                     EFLADQLTLSLDRLGLATLDVCLLHNPEYFLSHATRLGGDESRDLPALRQEFYARLQR
                     AFEYFETQVQAGRLRGYGVSSNTATSAPDDPGATSLSHMIDAAKLAATTVGAPSHHFQ
                     VLQCPMNVYESGAALIKNTGIHNGSTLLEEAMRERIAVLVNRPLNAMPVQRGGVIRLA
                     DVSVPAPEADFEAQRKKVAALEEEYRNSLAPAVAHSGQGMLPADFFRWADELTRIRGQ
                     VQGLEHWEQIEQQMIAPHVNQVLRALAEAFTGNVAEQWESWRDRYVPELLALLRILHR
                     EASEKSRLRSDELHHTMNPLLPEQRRTATLSQKALWVLASTPGVTCVLNGMRTPAYVE
                     DALTILQWPPLPEWRSVYDRCAGKK"
     gene            complement(65018..65242)
                     /locus_tag="PK589_02585"
     CDS             complement(65018..65242)
                     /locus_tag="PK589_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18510.1"
                     /translation="MKRALFAFVAAAILVAFTAPSFAGDDKKKEEKKGGHFSQTLFGD
                     DKKKEEKKGGHLSQTVFGEEKKKDEKKGGH"
     gene            65634..67076
                     /locus_tag="PK589_02590"
     CDS             65634..67076
                     /locus_tag="PK589_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HNJ18511.1"
                     /translation="MTTDDIGPYSNYRLTVRLALLNKPGIFAKVAALLAEEGANLGAV
                     DIVSANAERMIRDITFDVQNEAHGDRVLERLEALPEVNVLSASDRIFLLHLGGKIRVQ
                     SKVPVTTRNVLSMIYTPGVGRVCKAIAKDPVKAYAFTSKSNSVAVVTDGSAVLGLGNL
                     GPAAALPVMEGKVMLFRELAGIDAWPICVSTQDPEEIIRIVRGIAPGFGGINLEDISA
                     PRCFEIERALKTSLDIPVMHDDQHGTAVVLLAALTNALKVVGKRMEDVRIVVNGLGAA
                     GTACCRILLAAGACHLVGCDKEGIVLMGEAEELRACRTDLQACIRRDQPRGTIHDALR
                     GADVFIGLSVGNVLEKADLDNMNSDRIVFAMANPDPEIDPKIGDEVSRIFATGRSDYP
                     NQINNALSFPGIFRGALDVQASEINEAMKLAAAQAIAECVPTEALSEDYIIPSVFDRD
                     VVPRVAKAVAAAARTTGVARRRIKIEDDPR"
     gene            67244..68503
                     /locus_tag="PK589_02595"
     CDS             67244..68503
                     /locus_tag="PK589_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HNJ18512.1"
                     /translation="MTPTTIQLIGTLLFAVAILHTFATAFFEHLAHTRPAHAGLWHLL
                     GEVEVAFGFWALVLIVAMFVTDGAAAATHYLDSRSFTEPLFVFAIMVIAGTRPILQTA
                     MTGVRIVSSAIPLPQGMRVYLTVLALVPLLGSFITEPAAMTLAALLLRDVFFRHGCST
                     NLKYATLGVLFVNVSIGGTLTPFAAPPVLMVAGAWQWDIAFMLGTFGWKAALAVLINA
                     FGATMLFRRELSRISTAIDQREENRAPLALVITHLALLAMVVVFAHHPAMFLGILLLF
                     LGIAQAYEQYQDRLILREGLLVSFFLAGLVVLGGQQQWWLQPILLGMGADAVFVGATI
                     LTAFTDNAALTYLGSLVEGLSDAFKYALVAGAVTGGGLTIIANAPNPAGLAILRTYFD
                     DEAVHPLRLFLAALPPTLIAAAMFWCL"
     gene            68615..69244
                     /locus_tag="PK589_02600"
     CDS             68615..69244
                     /locus_tag="PK589_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNJ18513.1"
                     /translation="MAFSTNRLFKYQRGMRRRIGMLRAKNIDSMEFAVDFMMQERVDS
                     YFRIEPALEEVERSLGQIEDELDLVKDVSGAIRLESRLEFVEDRWDELDSEIRERPRR
                     RRRKINLADFLKAAGGEGNPAGTSSEVTNAYDAYQLLGLEFGTPLADVTTAFRQRAKE
                     LHPDARNGDRSSEPELRRILEAYQFLKEYLSLSNTEPPISRGAEYRPTE"
     gene            69248..70453
                     /gene="rnd"
                     /locus_tag="PK589_02605"
     CDS             69248..70453
                     /gene="rnd"
                     /locus_tag="PK589_02605"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HNJ18514.1"
                     /translation="MATLDPDQTFITSDHMLDRLCDQLANSAVIAIDTEFMGEDHFIP
                     RLELIQVAAEGVAAVIDFPAVQDSAPMVRFWELVCDARIEKVLHAGRQDLELFAHHAG
                     RLPKPFFDTQIAAAMVGYGAQTAYANLVQRVQGVKLDKAHTFTNWSQRPLSKDQLVYA
                     LDDVTFLLPIHRHLRQKLSVMGRLDWVDEEFSRLEVSLGAQARDPQERYQRIRGWDSL
                     KPRAAGVLRDLAAWREGEARRRNVPRGRVMRDEVLVQLARQTPRTLDQLRGMRGMYSS
                     EVERNGQALLATMQQALARPESEWPEVPRDRKPDPESTGVLELLQAVLKSRAAMENIA
                     PTLIATTGDLQALVERTSPVEEVDIPVLRGWRRQLVGETLLSVLSGNLKVWIDPQIGK
                     LRFGHLDHP"
     gene            complement(70487..70729)
                     /locus_tag="PK589_02610"
     CDS             complement(70487..70729)
                     /locus_tag="PK589_02610"
                     /inference="COORDINATES: protein motif:HMM:NF040329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18515.1"
                     /translation="MLAFDSVDRVSLLGRVLQRLNLRFPSLFVLFAVLTLVDVIVPDV
                     IPLADEIGFALLTLLLGMWKDRRHQPRQRTAGKAFR"
     gene            complement(70790..71140)
                     /gene="arsC"
                     /locus_tag="PK589_02615"
     CDS             complement(70790..71140)
                     /gene="arsC"
                     /locus_tag="PK589_02615"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248978.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNJ18516.1"
                     /translation="MAEITIYQKPTCSTCREAVRLVRESGQPFTAVNYYEQPFTKSRL
                     KELLKKAGLSPRDVLRTKEDLYKSLKLADSTLSDDHLLDQMIAHPDLIQRPIVVKGDK
                     VMLARPADTVTELL"
     gene            71241..71615
                     /locus_tag="PK589_02620"
     CDS             71241..71615
                     /locus_tag="PK589_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18517.1"
                     /translation="MPIFGTDTSDQTGKFSCATATQRTLKDLRTKRKGQPVFVVGHRL
                     ERKGKEATFEVFNDRLAIIRFEDDVRLGYDPFELLLPTEIDDKGVAYFEIRTCRLCEQ
                     FFPLTADECDEEEERTTCPECA"
     gene            complement(71673..72056)
                     /locus_tag="PK589_02625"
     CDS             complement(71673..72056)
                     /locus_tag="PK589_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18518.1"
                     /translation="MGRFRSLALGVLMVIGLNVPAWADDEQVFFAVVSDVPKDKTRIS
                     AKVLQDGASSDIKLIPSESVVANPVWRTLEVCHALKIEGMKIGDGLKVMSARVLDASM
                     LPMGLQGFAGDCLIKKAVEIAPLAE"
     gene            72527..73660
                     /locus_tag="PK589_02630"
     CDS             72527..73660
                     /locus_tag="PK589_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNJ18519.1"
                     /translation="MPHDFMPGLAGVPAAKSAISDVDGIRGVLEYRGIRVEELCQHSS
                     FLETSYLLLFGRLPTATELTQWVDDVTHHRRIKFRIVDLLKVMPEHGHPMDALQAAVA
                     ALGMFYPGRNVKDVENNYWSAVRLIAKLPTIVAAWARLRRGDEYIPPRDDLPFADNFL
                     YMLTETVPHPLWSAVFDDCLILHAEHTMNASTFAGMVTASTLADPYTVVASSIGALKG
                     PLHGGANEEVVTMLKEIGTPAQARAAVEARLGGKNKLMGFGHRVYKVKDPRATVLQDL
                     CMRLFNVCGTSPLYEVAVAVEQLASERLQGKGIYPNVDFYSGIIYDKMGIEVDLFTPL
                     FAMARVSGWLAHWLEQLRENKLYRPDQIYSGEHDRHYVPIDRR"
     gene            73950..74405
                     /locus_tag="PK589_02635"
     CDS             73950..74405
                     /locus_tag="PK589_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNJ18520.1"
                     /translation="MAIVRWDPFRELEEMSDRLNRMITRPGAAQTATQGKEVMTVADW
                     APTVDISETEAEYAIKAELPEVKKEDVKVTVEDGVLTLQGERKQEKEDKGKKYHRIER
                     SYGRFVRSFTLPDTVDESKVKAEYTDGVLHLHLPKSEKAKPKQIDVKIA"
     gene            74612..76069
                     /locus_tag="PK589_02640"
     CDS             74612..76069
                     /locus_tag="PK589_02640"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNJ18521.1"
                     /translation="MMKNASRTPKSTASATTATRMERDTMGELPVPSNAYYGVQTARA
                     IENFPISSLRIPRAMIRAMGLIKRAAASVNHSLKLLDKKPADAIIRAATEVVDGGLDA
                     EFPVDIFQTGSGTSTNMNTNEVISNRATELLGGARGSKLVHPNDHVNLGQSSNDVIPT
                     AIHIAASETIQRQLLPALTQLHKALSGKAREFDKIVKIGRTHLQDATPVRLGQEFGGY
                     ARQIELGIARMKRAQAALSEVALGGTAVGTGLNCHPKFAGKVMALISKETGCSFKEAV
                     NHFEGQSAQDSLVEASGELRTLAVSLMKIANDIRWLGSGPRCGLGEINLPETQPGSSI
                     MPGKVNPVIAESVTMVCAQVIGNDVTITVGGQAANFELIVMLPVMAYNLLQSIELLST
                     ASTNFAVKCIAGIKANEERCKSLIEESLAMCTALAPVIGYEAAAKLAKDAYKSGKTVR
                     EVAREQNVLPEKRLNQLLDPWRMTQAGGPVGSAGG"
     gene            76251..77621
                     /locus_tag="PK589_02645"
     CDS             76251..77621
                     /locus_tag="PK589_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ18522.1"
                     /translation="MSATGSETSGHVVVVVGAGPAGMALAKKMADAGHEVIILNRDIK
                     FGGLAEYGIFPSKLKLRGGLKKQYWDMLEQPNVHYFGNVSVGQGKDLTVEELRGLGAS
                     AVAFSIGAQGTKAIGVEGDSAKGVFHAKDVVYHFNRLPGFGDRPFDMGKHVAIIGVGD
                     VMVDIAHWLIRYKKVERVTAIARRGPVERKYNPKEIRAVCSNMDHEGIAQEFARIKDR
                     LAAVGQNADEVLANLTAEFTKCEPTGSSSKMGFRFLASPKRVLVDANNRVRALEMEEN
                     KLEPKGEDTAAVGLKQLYEFPVDSVVFAVGDRVDETVGLPYKNGVYVTNPTKTANDPD
                     DSLFQAYDEKSGQVVDGVFLAGWARKASEGLVGIAKRDGDWCAEVISRYLTTKSVQSV
                     SAVDQVLSKLQAILKERKSKPVDLEGLKVLDTVEKAHAASPEAIGEFKFASNADMLAH
                     IERGRA"
     gene            complement(77637..78497)
                     /locus_tag="PK589_02650"
     CDS             complement(77637..78497)
                     /locus_tag="PK589_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNJ18523.1"
                     /translation="MKSKSIGILTKPKFPEVKATVQAVVSWLRSRNIDVLLDTTSTTL
                     LGEQGGFQKTQLASKADVLLVLGGDGTMLNAARLAGERGIPILGVNMGGLGFLTEVVL
                     ENLYPSLERVFANDFVLDERLMLRTHVHRHGETVARGVVLNDVVISKGTLARMIELKI
                     AIQGQFVTNLRGDGLIISTPTGSTAYSLSAGGPIINPAVPSLILTPICPHTLTHRPLI
                     VPASAQIEVVLTSKDDGAMATLDGQVGVALSQGDTVEIHASEHLTRLIRFPESSYYEV
                     LREKLKWGDG"
BASE COUNT        17371 a        22836 c        21874 g        16729 t
ORIGIN      
        1 cagaactcga caagactgtg atcgagtcga tcggtgaccc gttgacgcac ctcgtgcgca
       61 actcagccga ccatggattg gaaccacctg acgagcgcct cgccgcggga aaatcagagc
      121 agggcaccat acggctcaat gccttccacg aaggcggcaa tatctgcatc acggttgaag
      181 atgatggacg cggattgaat cgagacaaga ttctggccaa aggcatcaag cagggcctca
      241 tcgctgagac tgacaaactc tccgatgaac agatctggat gctcatcttc aaaccgggct
      301 tttccacggc cgaaaaggtc acggacgtgt ccgggcgcgg cgtcggcatg gatgtcgtca
      361 aacggaatat cgaaggtctt ggcggcaccg tcagcattaa gaccagcgca ggcaaaggca
      421 ccacttttac gctcaagctt ccgctgaccc tcgccatcat cgagggcatg accgttcggg
      481 tagggaggga cacctatatc gttcctctcc tgtcgattct ggaatcgatt caacctaagc
      541 gcgagatgat taagaccatc gtcggaaaag gggagttggt caatgtacgt gggacctatc
      601 tcccgctcat gcgcttgtat gacgtgtttc ggttggaacc ggagctgtcc gaccccacaa
      661 aagccatttt gttgatcctc gaaactgaag gcgaacgcgt cgccgtcatg gtggacgaga
      721 ttctcgggca acagcaggtc gtcattaaaa gtatggagca gaacttccgc aagattgaag
      781 gagtggccgg ggcgaccatt ctcggtgacg gcaccgtcgg ctttattctc gatgtgcgtg
      841 gattactcaa tattgcgcgg caaaggaaag cgaaggcggc ataacaaccc actcatgaac
      901 tgcatcggca cataagattg tacgaacagg gcgaggagga cacgatggca tcacaggaat
      961 cggcaagcaa agagtcgaat cagcagatcg ggatcacaac ggacggcagt caattcctga
     1021 cgttccaatt gggggaggaa ctgtacgggg tcgatattct ccgcgtacag gaaatcaaag
     1081 ggtatacggc ggtcacgcgt attcccaata cacctcctca tatcaagggc gtgttgaatt
     1141 tgcgcggtac cattgtgccc atcgtcgagt tgcggaccaa gttcggcatg ccgacgattg
     1201 aatacacgat gtttacggtc atcgtggtcg tggtcgtaaa agaaaagatc atgggcctcg
     1261 tggtggatgc ggtttccgat gtgctcgaca tcgacaagaa agatattcaa ccggctccgc
     1321 agtttggcgc ccaggtcgat gtcagctttc tgaatggaat cggtaaggcc ggcgacaagc
     1381 tcgtggcgct gttggatatg gatcgtctcc tgactgacgg cgacttgcag gaaacggaaa
     1441 aagtcgcggc ataaccactc gaaacccagg gagagaagga catggcatta caggtcgaat
     1501 tgttggaatc cagttttaaa ttggtggcgc ccaaaggcga ggagttggtc acccggttct
     1561 acgaacggct cttcaaaaaa tatccggccg tgaagccgct ctttaaacac accacgatta
     1621 aggaacagaa gaagaagctc ctggcgtctc ttgtcctggt catccagaat ctccgccgcc
     1681 cggagaagct tacgcctgtc ctgcaagata tgggcgctcg acacgttgga tacggagcga
     1741 agccggccca ttatgacgcg gttggagaaa acctcctggc cgtgctcggg gaagtcgccg
     1801 gaccagcgtg gacgccgcag gtcaaacaag cgtggacaga ggcctatggc gccatcaaga
     1861 ccatcatgct cgctggagcc gaacgtgtac acaacacaca aggagaaaag acgatgagta
     1921 agccgaaggg gaaaaaaggt accgcagctc aagccgatat gacggaattt tacctgggag
     1981 cgcttgatca gtcgcagaat aacattctcc tctgtgatcg taacctcgtc atcacctatg
     2041 ccaactcgac ggctcagaag acgctggcca ccctggagcc ggcaatcaag aaggtgttgc
     2101 caaagttcaa tctaagtact gtggtgggca cctgtataga cgatttccat ttagatccca
     2161 gcaaacagcg cagaatcttg tccgacccgg cgaacatgcc gcaccgcgcg gacatccaaa
     2221 tcggcccgtt gacgctgagc ctattggtca ccgctgtcat gagcccaagc ggagagtatc
     2281 tcgggaatgc gttggagtgg gccgatgtaa ccgagaaacg ccgactcgag actgaaatgt
     2341 cccgtctgaa ggcttccctc gataacgcgc ggtcgaacgt cttggtctgc gaccgaagct
     2401 acacgatcgt ctatgccaac aaccagtctg tgacgacatt acgtcgactg gaaacggaaa
     2461 tccagaaagt catgcccgcg tttgcagtgg ccaaggtagt cggctcgaac atagaccagt
     2521 atcacaaaaa tccggcccaa caacgcacgt tgctggataa tcccaaaaat ctcccttaca
     2581 agacggaaat caagatcggc ccactgacac tggaccttgc cgttgccgcc atcatgaacg
     2641 aaaagggaga ctacctcggc aacgtcgtag agtgggctga catcagcgca cagaagaaag
     2701 cgcaggtcga aatcgacaag ctcattgagg cggccgcatc gggtcagctt tccgaacgga
     2761 tcaatgccgc ggaattcgac ggcttcttta agacactgtc ggagggagtc aataagatgc
     2821 tcgacgcagt cgtaacgccc ctgcatgaag cgcagacggt ccttacggcg atggcggcga
     2881 acgatctcac caagacgatg accggtcatt atcaaggaga gttcgagcag atgaaaagca
     2941 gcctcaacgg tgccttgaac acactgagca cagccatctc gacggtacgc gacggtgctc
     3001 aaagcgtgac cgcaggcgct gagcagatca ccaaggggaa cgaagacctg tcccaacgca
     3061 cctcggagca ggctagcgcc ctggaggaga cctcggcctc catggaagaa atgacttcga
     3121 ccgtgaagca gaacgccgac aatgctaaac aggccaatca gttggccatc gctgcacgcg
     3181 atatcgccga caagggcgga tcggtgacgg tgcgcgcggt agaagccatg ggcgaaatca
     3241 acaagagcag caagaagatc gcggatatta tcacggtgat cgacgagatc gcgttccaga
     3301 caaatctcct ggctttgaat gccgccgtgg aagcggccag ggcaggggaa catggtcgcg
     3361 gcttcgccgt cgtcgccgcg gaagtgcgca acctggcgca acggtccgca actgccgcga
     3421 aagagatcaa agggttgatc aacgagtcga tccagcgcgt caacgacggc agcgagttgg
     3481 tcaaccaatc cgggaagacg ctggaagaaa tcgtcagctc agtcaaacgc gtcactgaca
     3541 tcatcgctga aatcacggcg gcgtctcagg aacaggccca gggcatcgac caggtcaata
     3601 aagccatcat gcagatggac gaaacgaccc agcagaacgc agcattggtc gaagagacca
     3661 cgtcggcgag tcagtcgatg aaagaccagg cgcaggaatt gatgcgtcag gtagaggtct
     3721 tcaagatcga ggcaggcgag acccaacggc agtcgaacgc ccattcctcg gtcgggaagt
     3781 ctccggcaaa aagcgcgatc gttgccacaa agccagccct gaagaaaccg agcttcacgg
     3841 ccaggccggc agatgccaag cagccggtgg ggacccctgc tggtaacgga catgatcgcc
     3901 ataagaagga tgacgacttc gaagaattct agcctcctga atgcgcgggt gggcagatct
     3961 tgcccacccg cgcggctgcc tcgcatgacg tttcctttcc tcagcttcgc ctcacgtgtg
     4021 cagtgcccac cccctcaaga aatagcgtga cacaaccgat acatgatggt gcgggtgtct
     4081 gtatgccgga cccgtttcga cggttccgac gattccacct ctagcccgag gacgatcatg
     4141 gacgtcaaaa cgcttctttg gtacctcgtc ctgtccagtc tgactgtcgc gtatctcgtc
     4201 gtgttagccg gcgtcaaagc ggccaagtct cacgatgtca cgcatcattc tcatcgcatg
     4261 attgttggtt gcacgatcgt cggtatctgg ctggtcgcct acgtcctgaa acaagccctg
     4321 tttggacgag agtccttcca ggggccggaa agtgcctatt ggtcggtgta cgtcccaatt
     4381 ttcgtcacgc acatgctgct cgctgtcacc actattggcc tgggtgcgta caacctgtat
     4441 atggggctcc accggctacg gttcggaagc gtcggagcca tggtggccgg catgaccacg
     4501 catcgccgaa tgggcaacgc gcttatctgg acgtttagcg gcacgatggt cacggcctac
     4561 ctcgtatatc tcatgctgtt tgtctggtat cggagctgag acgatggact atcccatttc
     4621 gaagaaagag tacgatcaaa tccggaccct gctctatgac gagagcggca tctctctcgg
     4681 agagagcaaa cagtcactcg tggtgtcacg cctcacaaag cggttgcgcg acctccaatt
     4741 gggcgatttc tcctcgtact atgagtatgt gacggaggac aacagcggcg aagaatttac
     4801 gcgcatgctc gatctgctct cgaccaacaa gaccgacttc tttcgcgaac cgaagcattt
     4861 cgattttctg cgcgagcgca ttcttccttc tctcgagaag gagaagcgta tccgcatctg
     4921 gtcttcagcc tgctcgaccg gcgaggaacc ctacaccatt gccatgaccc ttcacgaagg
     4981 ggtccagcag ccggcccagt ggaacttcca aattctggct tcggacattt cgacacgagt
     5041 tctggcccat gctgccaagg ggctgtatgc ggaagaccgg gtgcgcgagg tgccgcccga
     5101 aatcgtcaaa catcatttcc ttcagggacg gggagatagt gcgggactgg tgaaggtgaa
     5161 gccccacctc tccgccatca tcaagttccg gcggttgaac ctgatggacg atcattttcc
     5221 catcaaagcg ccgctggatg tgattttctg ccggaatgtc atgatttatt tcgatcgccc
     5281 gacacaggaa cgcctggtca acaagtttta tcaccatctc aaaccgggtg ggcatctctt
     5341 catcggccac tcggaaagtt tgcagtgggt gtctcatccc ttcaaaacca tcgcgccgac
     5401 gatctattgg aaggaatcct gagagacatg gccatcatcg acaccgatgc gttctcgcat
     5461 atccggcgca tgcgcgatgg acgattcccc cacgaggtgg cgtgcattct gccgggagag
     5521 tttttcgtga gccgggaacc gatggtcgtc tatacggtcc ttggttcctg catctccgcc
     5581 tgtatccggg atcccatcgt gggggtcggc ggcatgaatc acttcatgct tccggctccg
     5641 aaggagcatc agtccggcga tgcctggggc ggagaatcga cccgctatgg gtccttcgcg
     5701 atggaacagc tgatcaacga aattttgaaa cgcggcggac tccggcaccg tctggaagtg
     5761 aaattattcg gggcagggaa gatctatgac ggtaacatcg acgtcggggc acgcaacacg
     5821 gaatgggtgc tccaatattt gaagacggaa ggctacgccc tcgcgaaaag cgacctgggc
     5881 gatgtgtatc cgaggaaagt gtactacttc accgactcgg gccgcgtcct gatgaagaag
     5941 atcgagcgcg tgaaaaaccg cacgatctac gagcgggaga cggaatacca gcatcggatc
     6001 gaagtcgaac cgacggtgca acaagacaat attacgttgt tttgacacag gagaccggtg
     6061 gcggcacaga acatgcgcac accagaagac caggagatgt gtggcaatgg ctaagattcg
     6121 agtcctgact atcgatgatt ccgcactgat gcgacaggtg ttggctgagt tgttgtcaaa
     6181 agatcctggc atcgaagtga tcggcagcgc accggatccc tatgtcgccc gggaaaaaat
     6241 taaggcgttg aatcccgatg tgctgaccct tgatgtggaa atgcccaaaa tggacgggct
     6301 cacgtttctc gaaaagctga tgcgcggacg cccgacgccg gtaatcatgg tcagctcgct
     6361 cactgaggct gggtgccaaa cgactctccg ggcgctggaa ctcggtgcgg tcgatttcat
     6421 cacgaaaccg aaaatcgatt tgcgagaagg catggaccag gtggcgcagg atctcattgc
     6481 caaaatcaag gctgccgcaa cggcctccgt gcgtcccacc acggcggcgg catcgagcac
     6541 ctcgcgtccg acatctctca attcggccat gatcaagacc acggatatga ttattgcgat
     6601 cggctcctcc acgggtggga cagaggcggt caaagacgtg ctccaggtgc tccctcccaa
     6661 taccccgccg atcctcatca cccagcacat gccggaacgg tttacgaaga cctgggcaga
     6721 tcgcatgaat cagctctgcc gcatctccgt caaagaagcc gaggatgggg atagcgtgct
     6781 gccggggcat gcgctcgtgg cgccgggcaa ctaccacatg accctggttc gcagcggcgc
     6841 gcgctattcg gtccgcatca accaggatga accggtcaat cgccatcgcc cctcggtcga
     6901 tgtgatgttc gactccgtcg cccagtatgc aggaggcaac gcggtcggcg tgatcctcac
     6961 cggcatgggc ggagacggtg ccaaagggtt gttacgcatg aaagaagccg gcgcctttac
     7021 catcgcccag gacgaggcct cctgcgtggt gttcggcatg ccgaaagaag ccattaagct
     7081 tggtgccgcc gacatcgtcc gtccgcttgg cgacatcgcg tcagcgattt tgactcaagt
     7141 cacccgtgcc tgatcgttca ccttttgcac taagaggagc agcaatgaaa atcaccagcg
     7201 aaatccacaa tcataaagtg accttaaaac tggaaggcaa ctttacctat acccagcgga
     7261 agccgtttca ggaaatgctg aagtccgtca gcgtcgatcc gatggaacac atcgtcatcg
     7321 atctgtcgca ggtggcattc ctcgacagcg ccgcgttggg actcctgatg atttcccatc
     7381 ggcagttgca ggccgaaaag cgcaccttgt cgttggccta tcctcaggcg acggtgcggc
     7441 agatcatcga gctggcgaat ctgcacaaga ccattccgct gatcgaatcg ccagcttcca
     7501 ctgtgacgaa gaaaagcgcg tgacccttct tttctggaga tgaaaggaag cacgcatgca
     7561 gattactgaa cggcgagtcg gggagtccgt cattctggac ttggtgggcg aactgacgta
     7621 tgcgaatcga gccacgttca aagcgtcggt ggagcgcagc agagccggcg gcgtccgtca
     7681 tctgattttg aatatgcaag gcgttcggtt cctcgacagt tcggcgctcg ggacactggc
     7741 gttactgacg caaagcctca gcgcgacgcg gggcacgatc ggtctcctga acccccagag
     7801 ttatgtcaaa gaaatcctga ccctggcgaa tttgcatcaa ctgctgccgg tctaccattc
     7861 ggagcaggac gcactggctg cctgccggtt gcccaaagcc gggtaaggga cacatcagga
     7921 gcacgacacg tggctgaacc cgcacgacgt atcgatccgc aatccggcac tgacagcgac
     7981 gcgaagccgg caccggtcat ccttctcgtc gacgacgacg agatcacccg gaccggtatg
     8041 gccggacgac tgaaacgctt ggggtaccgg gtcattgagg ccggcgacgg aaccgccggc
     8101 ttgaccgcca tccgtgctca acgcccggat ctggtcatct tggattggat gatgccgggg
     8161 atggacggcc cgagtgtctg cgaagcgatc cgcgccgacc ccgagttgaa atccagccag
     8221 gtcgtgctca tgacggcaca cgaccgaccc gaacagatcg cggaagggct ctcccgcggg
     8281 gccgatgact tcctgagcaa agcggcgagc aaacaggaag tattggcgcg ggtgcaagcc
     8341 agccttcggt cgagcgccct ggtacgtgaa atcgaacaca cgcgcgacga cctcgatcgc
     8401 tctcaccgtc tgctgtcggc gaaacaagcg gagttggaaa gcgagctgca atcagccgcg
     8461 gatttcgtcc gcgcgcaact ccctctgccc ggcatgccgg ccacgggagt cagtatgcag
     8521 tgggcctacc agccatctct tgccctgggc ggcgacttgt ttcaagtgtg ctcctgggga
     8581 gccgatgctc tagggttata catcctcgac gcgtcggggc acggagtggc tgccgccttg
     8641 cgagcagtgg gacttatgag ttttctccgt gaagacaatc tggcgaaagt ggtcggaagt
     8701 tttgatccgg gagcgatcat tacggaggcg aatcgtctgt ttccgctgac gcaggacgga
     8761 gaatatttca ccttatgggt ggggcgtctg gacatctcct cacgtatgct gtcctacgcc
     8821 accgcaggcc atagcggagc atttctgcag gcagccggcg atggatcccg atggctctcc
     8881 atggccagtc ttcccttggg attcgatccg cacagtgtgt ttgaaagcat gcataccccg
     8941 ttgctgtcgc tggatcgtct gtatctgctg agtgacggca tctatgaggc cccttcaccg
     9001 acaggggaac tctggggacg agaccgcctg caagcaacgc tggaagcgca ccggagccac
     9061 agtcttggac aaagcatcgc tgacaccatg gccaccgccc gacagtggct ggcaggggag
     9121 attttccccg atgacgtcgc tctgctgggg ttagagatgc tggatactct gttgggccaa
     9181 aaggaaaggg cgtaatggac tcgcaaccgg tcatggacct ggctgccgcc ctcgatcgct
     9241 tcgacgggga tcaggatttg tttctgaccc ttgccggcct ctttgtggaa cgctcggggc
     9301 aggcgctggc cgcacttcag accgctctgt cggcccagga ccttccggtg ctggtcaagg
     9361 aagcacataa attaaaaggg tcggccatgg agttttgtgc gcgtcccgct gtcgctgcag
     9421 ctgcacatct tgaagaagcc gcccgaaagg ccgccgcgca ggacatcccg gcgctggctg
     9481 agcagactcg tgcagaagtc gaacgcctga cggccgcact ggctgagatc atgcaaaaag
     9541 gctttccctc atgattggtc acgccgcatc gcccacgatg ctggtcgtcg atgcctctcc
     9601 cgagacctgg gcaatggccc tggaggaggg gaaactccga gggatctccg tcattacagc
     9661 gccggatccg caagtggcat tggccatgat tgatatggct gcccccgata tcctcatgac
     9721 tgatctgttc ccggctgacc aggccggtgt gttgctgatc cgtgaactcc gcatgcggtc
     9781 cccgaaaaca gccatccttg cgaccggcga gtctggagat gctgagacga tcctgaacgt
     9841 agtacgggcg ggcgctgggg actatctacg gaagcccctg ctcgtggaag aactgggctt
     9901 ggcgctggat cgggcgcttc gacagcttcc gtcgaccgtc gaaaatatcg ctggcgtcga
     9961 acaggtggac taccgtctgg tgttaggcac caatccggat catgtcgagg cctgtgtgac
    10021 ctggttgatt cagcagaccg ccctgacgct tcccgagatg cagcgcctgc atcttcgcac
    10081 caccttgatc gaactcatcg tcaacgccgt agaacatggc agtctcgaga tcctgtacca
    10141 ggaaaaacac gcggcattga gcagcgacca gtttgagcgg ctgattgagg agcgccgccg
    10201 ccacccgcgg tttgccaggc ggcgtgtcgt ggtgcaggcc tcccatgaca aaactcgccg
    10261 gcgcctccgt tatgcgatca cggacgaagg aaagggcttt gcctggagcc gttttgtggc
    10321 cacacctgaa cagccctgcg acagtcgcca tcccaacggc cggggggttt ttcttgccaa
    10381 ggcgtttttc cccgatttga cctacaacga acgcgggacg gaagtgacgt tttccgtgcc
    10441 ggtgccctag gctctacaat ttcgacgcgt cctgcggctt gacgcgcctc cgcttcgcgg
    10501 tttatcttcc ctcgctgatt ccgttccctc ctgaccgctc acaaatgagg cgtctcatga
    10561 cttctctcac tcgtccggtg tttcttctca tgctatgtgc cttttcggct ggttgcagta
    10621 ctgccgagtg ggttcacccc aatcgcccga aggaccaatt taccctggac tataacaagt
    10681 gccaggccga caccatgcgc gaccctaaac tgcagcaggg cattcaactc ctgatcatcc
    10741 aggccacgga acgctgtgtg caaaaacagg ggtggcggct ggtagaaaaa gagtagccac
    10801 tcccgaatgg cgatcggcag gcaagccggc ttctgtgcct gtgtcagccc tcgccattcg
    10861 ctccgtgaca cgcctcccac cttttcgcat cacttgtctt cgagtcgttt ttgttgacgg
    10921 atcgatcaag gttcgttatg taatgaactg atcgaccatc cacctccgcc gccgtacagg
    10981 aaaccatgcc atgacacgca tcgatctcat cgccggagca cgcccgaatt tcatgaaaat
    11041 cgcgccgatc atcgatgcgc tgaatgccgc ccggtcgcgt ggaagccatc ttcgttttcg
    11101 actcattcat acagggcagc actacgaccg cgccatgtcg ggcagctttt tcgaggaact
    11161 gggtatcccg gatccggaca tcaatctgga agtcggttcc ggcacgcaag ccgaacaaac
    11221 gtcggccatc atggtgaact acgaaaaagt tctggccaaa gataaaagcc atctctgctt
    11281 ggtcgtcggg gacgtgacct cgaccatggc ctgttcgatt gtcgcccgca agatggggat
    11341 tccggtggct catgtggagg gtggtatccg ttcaaacgat tggacaatgc cggaggaaat
    11401 caatcgcgtc gtcacggatt ccattaccaa ctggttcttc accacgagcg aaacggccaa
    11461 tgagaatttg cgccgggcag gtgtcacaag cgaccgtatc ttctttgtcg gcaacactat
    11521 gatcgatacg ctgctcaaac atgcggaccg gttacggcct ccggcctgct ggagttctct
    11581 tgccctcaaa cccaatcact actttgttgt caccctgcat cgtccggcaa acgtggacgg
    11641 cgagcagcag ttgctggggc tgttacgggc cattgcggag ggtacccaag gtttaccggt
    11701 ggtctttccg gttcatcctc gaactgccaa aaacctccgc gaatcagggc aagcacttcc
    11761 gtcgatgcac tatgtcgatc cgttagggta tctcgagttc aattatttgg tgaagcatgc
    11821 ccgcggcgta attaccgatt caggcggcat caccgaggaa accaccgtgc taggcgttcc
    11881 ctgcctgacc ttgcgcgata acaccgaacg gccggaaacg gtgaccatcg gtaccaatga
    11941 actgataggt accgacccag cgaagctgcc gccggcgctg cagcgattga tggcagggca
    12001 gtggaagaaa ggcgcaattc ccccgctctg ggatgggaag accgccgtgc ggatcgtaga
    12061 acatctgacg accttactcg caaaacccta gtcgctaaga caacacgacc atggtctcct
    12121 gcttggtgcg gaaaaccgaa ccaacccgca gacgctctac ctgccaatag tcagtagcca
    12181 ttcaatggcg acaatctgac attgtgcttg gctcctcgcc aaattgttga cgagggttct
    12241 ctccgaaatc gtcaacttct cgcagagtac atacgatagg ccagagtcaa acgtccctag
    12301 aattctccta gcttgtcatt ctacaacaca tagccgcata cgtattcagg ccacggccgc
    12361 tatgtacaat cctgctcaat tcactgggct gactgacgac atcggccggt acgcaggagg
    12421 tatcgttccg tcctaattcc ccatttcatc atggttttag aagaacggtc ggagcgggta
    12481 gcgcaggcct gtcagttgca tactcctcac gacggtcaat ggaccaggcc atgtcacatt
    12541 ttcccctcgc cgaacaaggg gtctaggaac cgacggagat atgcgtgtcg cacgtaaaat
    12601 ctattgcact cattcaacta catattccgc actatcgtgt gcccctgttc gccgcgcttc
    12661 aggcggaggc tgcccgcaga ggctatgccc tgacagtcta ttcaggcgaa caacagccca
    12721 tgcccttttc gcatcggaaa ctgtccctcc gatacctgag tcatgacaag agcgggggct
    12781 gctggatgga agggcttgct gaggctgtcg ccggctcgga tatcatcatc gcgcctcatg
    12841 gcctgcaatg tctgacgatt ccttttctct ggctgcagcg gaaccgtctc tgcacatcct
    12901 ggatctggtg gggccacggg tacaattttc aggcgtcgac acagggttcc actgctgaat
    12961 ggatcacctc cgcgataaag aactttttac tgacacgagg cgatggggtc atcacctaca
    13021 cgcatggagg agctcaatac tggtgtaacc ggggcctgcc gcccgaacga gtggcgccat
    13081 ttctgaatac catcgatgtc gaggggctca gggaagcacg ccggcaggtg actgatgacc
    13141 aactgtcgct ggtacgagaa cgtctcgcgc tcggcaagaa acaggtgctg ctcttttccg
    13201 gacgtctgta tgcggaaaag caggtcgact tccttcttga agcattctcc aaaattcaac
    13261 aggcctgccc agaaactgct ttattgattc ttggcgaagg ccaggagcgt gcgcggctcg
    13321 aacagttgtg cagctcactg cacttgcatg acgtgcactt tttgggtgaa ataaccgatc
    13381 cgcatgaaac gagtatctat tttaaactgg ccgagctact tgtcataccg ggcctcgtgg
    13441 gtctggccat tgtccatgga tttgcgttca accttcctct gatgacgacc gaacgtccgt
    13501 ttcacagtcc ggaaatcgaa tatctgtcgt ccctgaacgg caggatgacc aggcacgaca
    13561 cggacgccta tgcggcggaa atcatccggg tcctctcgtc gccgcaggag atcgaggcca
    13621 tgcgatggca ctgtgccgag agcgcccagc agctcacatt ggcggcatcc tcgagtcgat
    13681 tcgtacaagc cgtcgcccag ttctccaatc cgacaggttc gcttacgagc aaccggcgca
    13741 gatcacagag aacatcgttt tcatcatccg ctgctacgcc atccctgagg agatagttca
    13801 tgctgtcacg acgtcaattt ctgcagatct cagccgcttg cactgcgtca gctctctgcg
    13861 cttcacttcc gcgattcgct caggcccagg aaacctttga aagccgggtc atttttgatg
    13921 agagcctcag ctgggtgacc gaacattccg cacatagtat gtgtcaacgg atgaagcaag
    13981 ccggtttcaa cgtggtgatg ccatgtgtct ggcatggccg gggaaccagt tggccatcca
    14041 acctggcccc ctgggacagt tacaaattgg acgaaatggc ccggtggaat tccagctttg
    14101 atcccttggg aaagctggtg gaaatcgccg cgcagtatca gattgaggtt cacccctggt
    14161 ttaatgtcac actccgtcag cgagaattcc tgccgcactt ctatgggccg ggcacgccag
    14221 ccgacgcatt cgacgtgcat cgtccggact ttcgcacgtt catctcaaat ttaatgattg
    14281 agtgtgtgtc gcggtatccc gttcacggca tcaatctcga ttacatccgt agcgtttcca
    14341 tctgccagag cccagagtgt gcccggcaat atcatcatca tacgaatcgc aacctcatgt
    14401 tggatcgatt gagctatggc gcctccgccg atgcgcggga agccattgct gattggaacg
    14461 aacaggcagt cggagatatc gtgcggcggg tgtccagcca tcttcggaaa aaccaaccca
    14521 atgttctgat cagcctgtgc ggacatcccg ggcatccgaa cttgtatatc caaggccaaa
    14581 aaagcattaa atgggccgat gaaggcttga tcgatgtggt ctacgacatg cgctaccccg
    14641 ccgaaccaaa ttgggaagag atccgatccg ttcagaaaac catgaagcgg cccgaagcct
    14701 acgccgttct gattggtaac tacgatctgg ccggagcgca tcaaatctcc gtgtccagct
    14761 cgggagcgcg cgtggcggct caaattgttc gaagccgtca agttgcaccg gggaatggag
    14821 taggtgtcta tctatatagc ttactcaacg acgaacagat cactgcattg cggtccggtc
    14881 ccttccaaac gccggcctcc ccgaaatggt cgcgcgcaaa agggttggat ctctcaacgc
    14941 cgaccggcct gctcgtccaa tagtccagtc gcatcgggat tgcgcgctct tcgaatttca
    15001 attacatcgc ctatcattgt gacgtatata atgcgtagct cgttcagagc tacgcagttt
    15061 ccttttcaga taggatgcgg ttcatggcct atgccttcgg ctttttgatt cttgcgctgc
    15121 tggtcaaaga taccgcaccc atcggcgatc aatacgtatt gcggaccctc tcgcagggtc
    15181 tctgtctgac agcgggattc tggtggctct ttacgcaggg caacatcatt gccatgcgac
    15241 gctactggct gattctgctc tacagcgcag tcctgtgcag cacggcgttt gtctccatgg
    15301 actcgacacg agtcttgctc caggttctct cgctggtggc agtaaccctg ttttttatga
    15361 gctattgtga acaagcccaa cagatgcctt ccatgcatga cacggccgct cgtatcgttc
    15421 tgtatgccct gctgccggtc tgcctgggaa gcctgttgct ctataaaatc gcaccgacct
    15481 tggcgttcga tcagacggtg gaacgcatgg tatgggacaa tccgcaccgc tttaaaggtc
    15541 ttttcggcaa accagcggga atcgcagccg cgtctggcat tctgcttggc ttgtgcgcgt
    15601 ttacaaaggt tcattggctg atccgtgccg tcggcgcact ctcctctctc atgtgcctgt
    15661 acctgacact ttcacggagc ttctgggtcg gatcgtctgt cgcgttgctg gtcacggcat
    15721 ttttgtatgc caaacggaaa ggcctcctgc ttggagcggg gattggaggg ctgctgctta
    15781 tcctggcgac ggtctccatc atggatacaa aaattccggg actcaaccaa tcacgatcca
    15841 tgcgatctga ctcgttggag aatctctccg gcagaacgac actctggacc ctcgcgcttg
    15901 atcgcttctg ggatcgcccg gtccttggat atgggtatac ggttggacac caggcgtttc
    15961 tctacagccg gaaggcgaaa ggggctgacg tggccggtga caatgtcgat ctgtttcgta
    16021 atgaaacatt ttcattgcac aacggatatg tccaggcgct gcttgattca ggaggaatgg
    16081 gcgcactgct ctacaccatt atcctcgcaa tcacggtatg gaggatcgta tggtacgacc
    16141 gcgtaaaggc ccatgccgcg gcattgtact ccctggtgtt cctatccatt gcgaacatcg
    16201 gagagacact cgtgtatacg ccggcaacct ttcacgctgc gttttactgg tatgtcgcag
    16261 tccttgcgct gggactccgc ttcacggtcg aaccaccccc agcgtcattt gctgagccca
    16321 tcgttctccc tgtacgtgaa cggacgacaa aatatccgat agtgtcacgg cctgaacctc
    16381 aggttcagca gggcaagact tctccgggcc tctgctgatt ttgccaacgc gcagatgatt
    16441 tcgcgctctg ccgacaggaa cgatgcagcc tgcgtgaatg gtaaaacatt gcgcgaacta
    16501 cccagcgtgt cggactaacg tctctgggac acgggcccat gctgtgaaga ctccagactt
    16561 gatatgaaga aatctgacga acattcgcat caaggccaga ccgatcaact ggtggttgcc
    16621 ggctccgcag tccttctttc atcgatattt ggaaacggac tcaattatct gttcggagta
    16681 ttcctggcac gatttctggg agccgatcaa tttgggctgt acgcattagc cctcgcgttt
    16741 ttcacaacct tgaccctggt ggtcccgctg ggaatggatg ccggcgcgat caagtttatt
    16801 tccgaatacc ggacacgtca ggattctgag ggggcgcagc gcatcgtgct catggccgga
    16861 acagtcacct ttttgaccgc cgcgttgacc gccatctgtc tggccggatt gtcctcactg
    16921 atcagcctgt cggtctatca caagccggag ctgaacattt tgctgctata cgtggcggcc
    16981 ggtattcctc tatacgcgct gacaagcatc ttattggcgt ctcttcaatc ccaccacacg
    17041 gttcggccac tcatcgtcgt aaggtatctg tgggagccga tcggcaagtt tatcctcgcc
    17101 ggtctcgtta tttcggctgg atgggggctg gccggcgtgt tcggcgcact tctcattgcc
    17161 atgctggtca gcctgactct gacgcttggt ttccttcgaa cggtcgtccg atttcgtcga
    17221 ccgatgatcg atctttggag ccgagaggaa gccgcacgat tgatgacgta ttgccttccg
    17281 ctgggggtgg cgaccgtgtt cggcgtcgtg gccccgcgcg ccgatattct gatgctcggt
    17341 tcctgggtga atgtgcacga gatcggtatt tatcaggccg cattccagac ggccgccgcc
    17401 ttggcgttaa ttcttggcgc attggaatcc tcactcaccc cattcttcgg ccaattgcac
    17461 gcacagaacg acatcgcggg cctacgccat atgtatcaga ctgcctcaaa actcgtcctc
    17521 ttatttactg tccccttgtt tgtgctcttg gctgtgttca gcgaggaggt gctgtctctc
    17581 tttggaacgg aattcagggc ggggggcaca ttgttaatcg tgttggcttc cggacaactc
    17641 ctcagtagcg caggaggatc acccaataat ctcctcctga tgggaggaca ctcacgactc
    17701 gtcatgtgga acacgatcgg cgtcggcctc ctggccttcg gggtgttcgc ctgtaccatt
    17761 cccctgtggg gagtgttggg cgcggctatt ggagcggctt ccacccaggt atttggcgtt
    17821 gggcttcgca tcctgcaggt gtggcggata tttcacattc agccgttcag ccagcgtctt
    17881 gtgaaaccac tcttggcggg tcttggcagc gcagtgttgg gcttgtcgtt gaaacctgat
    17941 gcgccggccc tgttgctccc gggcctcgga ttgatgatgg ggctggtcta tattgttctt
    18001 ctgattgccc tcaaactcga ccctcaggat cgccaggcgt tgaatgcctt ggtcagcaaa
    18061 gccaaaatga tcgttgcagt gcgtgcgtga tactgataca gactacttat gaagctcgct
    18121 gtcctgacga atattctcac accctatcgc ataccattgt ttcaggcgat ggccgaacag
    18181 gtggacgagt tttccatcat attgatggcg cagcaggagg agaaccggct gtggaaaatt
    18241 gaggatgtgc cttttccggt ttacgtgtta ccgggatttc acgtgcgccc ttggggtgcg
    18301 cccgtggccg tgcacttgaa ttacggcgtc accgcccggc tgacgcagct tgatccggat
    18361 gtcgtcctga atgccgggtt tgccgtagcc aacatagcgg cgtattggta ctgccgacgc
    18421 gcacacatcc catttatcca ttgggctcat ctgtccctca acgatggagc acaggcctcg
    18481 tttgttcggc gtcgaatccg gcactggcta atcggacgca gcgctggcgc agtcggcgaa
    18541 tcctcccacg ctcgagacgc gttcattcac tacggcgcgc cgaaagaacg aatattagtg
    18601 gcgaccatgc ctctggatgt gacgggtctt cacgatcgtg tccttgcgcg caaacaatgg
    18661 ccggaaacct ctacgccgct caacacgctt tcaaaaccag tcctgctctc aatcggccaa
    18721 ctcattccgc gtaaaggcta ccatgagctc ttcgccatct atgagagact cctcacgaca
    18781 catccgcaca catccctgct gatcgttgga gatggaccgg accgacagca tttcgaaaca
    18841 gtggtgcggg aaaagggatg gacgcgagta cactttgcgg ggtttgtgca ggcgcaggat
    18901 ctcccacgct actttgccct cagcgatgca ttcattttcc atacacttta cgatcctttt
    18961 ggcctggtgc tgagtgaagc catggcggct gaagttcctg tcgtctcatc gattcatgcc
    19021 atggccactc atgatttagt cgaggacggg gtcacgggtt ttcgtattga tcccttgcag
    19081 ccagaggctt cagcagagac ggtccggcat cttttcgaca tgtcttcaga ccaacgtacc
    19141 agtttgatcg acgcagccta tcgccgggtg cttccctgtg acagtcgaac gagtgcatca
    19201 agaattgtgg agtttcttag gagtttcagg acgccgcacg aaccccgtat ggcaaccaca
    19261 ccgagcccct cacagcgtat ttcatgacga ctcgcaacga caccatcgaa gtcatcggcc
    19321 cgactccgcc gccttatcat ggtgtgagtg tggcgacaca ggtgttactc gacgcgttgc
    19381 ctcattccca gctggccgtc gtccatctgg atacggcgga caggcgcggc atcgcccatg
    19441 tggatcaacc ggatctttgg gacgtcttcc tcttcattcg ccagtggttc gcccatctct
    19501 tcattctgct ccggaaacgg cctcgtattt gttaccttcc gctttcccaa agccgcgttg
    19561 gctttttgcg tgacagcctg tttatggttc cagcgtttct tacgggctca gccgtcgtgg
    19621 cacatctgca tggggcgaac ttttatcagg tctatgaggg cggcggaaca gcgtttaaat
    19681 ggtacgtcga tcaagtgctg aaacgcgtgg ccaaatttgt cgtgcttggc gacatgctca
    19741 aaccgatctt tcacaggtgg gcctctctcg aatcgattgc cgtcgtgccg aacggggtgc
    19801 cggaactatc gaccgatggg agccctggcc cacactgcgt ccggtcggca ggaccccgaa
    19861 tcgtctttct ctcaaccctc agccggaaca aaggtctctt cgtcctgctg gacgccgtgc
    19921 cgctggtggt gcaggagttt cccacagccg aatttcatat cgccggcccc tggtgggggg
    19981 ccgatactga acgtctggtg agagcgcaat tggagcaggg gaatgtgagc tcgcaggtaa
    20041 ttttccacgg ccagctggcc ggcgcgaaga agatggcgtt tctgcggagt ggagatctgt
    20101 tcgtcttccc cggcattcaa caggaagggc aaccgttgac ggtgttggaa gccatgtgcg
    20161 caggactacc agtcattgcc acggacagag gatgcctccg tgaaaccgtg atggaaggtg
    20221 aaaccgggtt tgtggtaccg ccgaactcac cgaccgcgat agcggagcga attcttctgc
    20281 tgctccgtga gccgcacatc cggcaaaggc ttggtgccaa tgctcgattg cgatatgaac
    20341 gtgaatatac gatgcctctc tatgccgcac gaatggcgga tctatttgca caggtcagcc
    20401 aatcggacta ccatgagaat ccgaaatcga tggattcgcg acactggagc atacgctgat
    20461 gaatacgctt cacgacatcg agcagttttg ggatgccaat ccttgcggcg aaaccttagt
    20521 gggcaaggat caggaatggg cgacgttctt caatcgctat gacgagtttc gttatcggac
    20581 tgaaggccac attctcgggg agctcgaccg cctgcagctc gctgggaaaa acgtgcttga
    20641 aatcgggatc ggtcaggctg cagatagtgc acagatcatt cggcgaggcg gacgctggca
    20701 tggtcttgac ctgacggcag aggccgtgtt ccgcgccaaa acgcgctttg aactcttcca
    20761 actcccgtac caggcggtca aacaggggag tgccacggcg atcccttatc ccgataaaag
    20821 ctttgacatc gtctactccc atggtgtgct tcatcacatc ccgccaatcc gtcaagtcag
    20881 tccggaaatt cgccgggtgc tgaagcccgg tggcaaatta gtcatcatga tgtatcacaa
    20941 acattcgttg aactatctgg tgagcatcaa catcgttcgc cgcttgctga tgatcggcct
    21001 ctttggtctg gcgaaaattg gcgcacgtcc gttgatcaca catcctctcc tggcaggcca
    21061 tctacaaaac gccgaggaat tcggcctctg gacctacctc cagaatccac tctttatgat
    21121 gcgaaataca gatgggccgg gcaaccccta ttccaaagtg tatgacgctc aggacattgc
    21181 ggcggacttc gccgagttcc gcctcgaaaa tacccgtgta cattttctca atgaacgcca
    21241 tctgccggtg ttgaaactgt tgccgccacg tgttcgaaca tatcttgcgt cgaagctcgg
    21301 gtggcacctg tgggccacgc tgggctaatc gcgcgcatct cgccgtagga gtatttcacg
    21361 acatgtgtgg cattgtcgga ttcatggcac aggatgccga atcggccctg accgagcgca
    21421 ccctgaccat catgcgcgat cagatgattc atcgtggacc tgacgatgga ggagtctacg
    21481 ttcgtcgcga aggcgcttcc acgatcgggc tgggacatcg tcggctgtcc atcattgacc
    21541 tctcgccact cggtcatcaa ccgatgagca ccgccgatgg ttcgctctgg atcgtgttca
    21601 acggggagat cttcaactac cgtgaactgc gggccgagct gccggcacgc attcaggcgc
    21661 agtttcgctc acaaacggat accgaggtga ttctctatgc cattcgagaa tggggccttg
    21721 atggctgttt gcagcggctg cgcggcatgt atgccttcgc agtctatgac agtacatccg
    21781 agatccttac cctggtccgc gacccgttag gcgtcaaacc gctgtactac acacacgacc
    21841 gtggacgtgt gacatttgct tcggagatca aagcgctcgc ggccatgccc ggtttcaaac
    21901 cggtgatcaa tgatcaggcg ttgtatcatt acctcacctt tgccaatgcc cccgcgccgc
    21961 agacgttctt tgaagggatc ttaaaacttg aagccggtac gtacctgact tgtgacaaga
    22021 agggatcaat gcgtcagcac cggtactgga atccatccac attccagccg ggatctgcgt
    22081 cactcacgga acaggactac gtggacgaac tgcgccgcct tctcaggcaa tcggttcgtc
    22141 gccgcatggt gtcagatgtg ccgttcggag tctttctcag cggaggcgtg gattcctctc
    22201 tgaacgtcgc tctcatggca gaactcctcg accggccggt tgaaaccttt tccatcggga
    22261 tcaaagacga tccgtccaac gaattcgact atgcgaggca ggtcgcggaa catttccata
    22321 cgaatcacca tgaggccgtc atctccgacg aggacttcat tcgttttctg ccgcagatgg
    22381 cctatttgca agacgaaccg gtcgcagacc ctgtctgcgt gcccatttat tacatctcca
    22441 aattggcgcg agaatccggg accccagtga ttcaagtggg ggagggcagc gacgaaattt
    22501 ttgccggcta ccagacctat catctcttcg atgactggaa tcgccggtat tatgaaccgt
    22561 atcttcaggc gccggaattc gtgcggcgtg cagtctacag ccttgggtcc cacgtctgtt
    22621 cttctgagaa acaggacgct ctgcgacgag cgatgttggg tgatccgctg tttctcggca
    22681 atgccattgc attctgggac aacgaaaagg ccggattgct tcaccaggcg tttcttccgc
    22741 ggcacacatc cagcgagtat attcgcaacc tgggtgggtc gttgggcatt gacgattcgc
    22801 tcctcaagat cattcacgtg gaactcaaga accgacttcc tgaattattg ttgatgcgag
    22861 tcgacaagat gagcatggcc aactcaattg agaccagagt gccattcctg gacgaggatg
    22921 tggtttcgtt tgcgctcacc attccctctg ccctgaaata taaacagaat caaccgaagt
    22981 acatccttcg gcaagcggcc cgtggaatca ttcctgacag catcatcgat cgaaagaagt
    23041 gggggttttg cgggtcggcc acgaatatgc tgtccacccg gcttaccgac tatgctcgtt
    23101 cgcgcatcct cgatagtccg ttgatgcacg aacgattcga tcgcaacgcc atcgaagtca
    23161 ttttcatagc tctccgccaa cagaagcgat ttaacagttt caaaatctgg aatctgctga
    23221 atctcgtcct gtggtacgag cgttggtttg cttccacccg ataagcctga tcgccaccct
    23281 ccggtggcgc gccgctccat attttttttc ttccttcgcc aactccccga agtctctccg
    23341 tagcactcct gtttttgttc cgtcgcccaa aattgaacgc cctgtgtacg attccatacc
    23401 tcgctcaccg gctcccgcca atccatcatc catatttcaa cgacttaacc acggtctgag
    23461 tggcccagga cttgctcaat ccccctcgcc aaccgggaac gacactgccg tacgatcctg
    23521 aagcgtttac tcaaccgaag gagtcatcat gatgaaacgt gcactcaacc tctcgctgag
    23581 tctcctcgtt cttgccgtgg gatcggttct gccgatcggg gaagagacca gggctgaggc
    23641 tgcactcgtg acctacgaat tcagcgggaa catcagcgaa gttcccggct ctctcttcac
    23701 gtcaggtgga acgggagcaa acgggttcaa cacgggcctc aagctgtcag ggacctacac
    23761 attcgatacg gccgcggcgg gattgctgcc gtcgagtttg ccgattcagt tgtataacaa
    23821 ttccgtttcg agcctgaatc tttcgattgg aaactactcg gcggtgcttc agggcggcgg
    23881 aaccaatttg acgaccgtga gcagcggcaa tcctgcgttt tattccgtga atgttggaac
    23941 ggcgggcaat ctcgtaaaag gactggcacc gtcgttattc tccatcaatc ttgcggatga
    24001 gaccggcacg gccttcagca gtgtgacctt gcccggcaat caagctccga acctcaacgc
    24061 cttcaatgtc aatcgttgga cactggcgtt tagtaacggc aagaccctgg tcggttcatt
    24121 ggatacgctg aaagcagttc cccttcctgc cacggtgctg ttgttcggag ccggccttat
    24181 cggtctggtt gggttgggcg cgcgcggccg tttcaaccaa tcctctgtgc gcgggtaacg
    24241 gcttgccgct gacgtaccac cgcatcatgt gacttgtgtt cgcacaagac cggttgtgag
    24301 aaaagtgggg cgcgcagaac attctgccgc ccgacttttc tcccaaggct ccctgtagca
    24361 ctttgctcta caaaaacacc tcgtacagcg cgaatcctgc aaaaattccg ttcaagcatt
    24421 gaccttccgg acgaacgaag gtagtctccg aaatgggctg ctcgtcctgc ctgttctttt
    24481 gttttgattc agtatcaatc caacactttt acgtatttgg tatcgcgaca ctatgaaaca
    24541 agtgattcaa aattttcgct ccggagtgct gaagatcgac gatgttcctc ctcccatgct
    24601 tcgcgatggt ggcttgttgg tgatgaacca cgtgtcactg atcagtcctg gcacggaaaa
    24661 atcaaccgtc caagtggcac agaaaagcct tctcggcaaa gccatggaac gtccggagat
    24721 ggtgaaaaag gtccttacgg ccatccaaaa agacggcctg aaagatacct tacaacgcgt
    24781 gttcgagcgc ctcgacaccc cggctgcact ggggtatagc tgcgccggaa ccgttgcggc
    24841 ggtcggtgct gatgcgtctc agttttcggt cggagaccgt gtcgcctgtg ccggtcaaaa
    24901 ctatgcctcc catgctgagg tggtgtatgt acccaagaac ctctgtgtga aactacctac
    24961 gacggtggat ttcgaagatg ccgcttgcgt gacgttggga gccatcgccc ttcaaggggt
    25021 tcgacaggca gagcctcgtc tgggggatcg tatcgctgtg atcggcctcg gactcctggg
    25081 ccaactgacc gtacaaatgt tgaaagcggc cggatgtcgg gtcctcggct ctgaccttga
    25141 tggcaccaag ctggatctcg cgcgccaact aggcgccgac ctcacagtac gtccctcagg
    25201 gctgatggat gccgcgactg gcttctcaga aggccatggg gttgatgcgg tgatcatcac
    25261 ggccagcaca aaggacaata gcccggtcga gattgccgga gcgattgccc gtaagaaggg
    25321 aagggtggtg gtcgtagggg cggtcggcat gaatctgccc cgagagccgt attacaaaaa
    25381 agagctcgac ttcaaactct ccatgtccta tggaccaggc cgctatgaca gcgcatacga
    25441 agagaaggga caggattacc cctacagcta tgttcgctgg acagaaaacc ggaatatggc
    25501 tgcctttctc gatctggtcg gcgacggaaa ggtgcagctc aaaccgctca ttacgcaccg
    25561 ctttgaaatc agcaaggccg atgatgccta ccgcatgatg acggaaggtc agacgcccta
    25621 catgggcatg ctgattacgt acccgctgga tcaacaacag gccctgcaac cttccatcac
    25681 agtcgtgagc gacaagccga ccggtcgatt gactctcggt ttgattggag tcggaaatca
    25741 cgtcaaagac atgttattgc ctcaattgca gagccaggcg gccgtctcta tccgagccgt
    25801 gtgcacggct tccggcatca acgcgaaagc tctcggggaa aaattgcacg caggctattg
    25861 caccagcgac tcccaggatg tgcttcgaga tccggccatt aataccgttc tcattggtac
    25921 ccgacacgac acccacggtc gtctggttgt gaaagccctg gaagccgaca agcacgtgtt
    25981 tgtggaaaaa cccctgtgcc taaccgagga ggagcttgac gccatccatg ccgtctattg
    26041 cgacaaggct gcgaagggac ttcgtctgat ggtcgggttc aatcgccgtt tttcccctca
    26101 cgcagagaag gcgcgcgagt tttttgccca tcgcgaaaat ccgctggtca tgtggtaccg
    26161 catcaatgcc gggaaaattc ccgccgaaca ttggattcaa gacccgtccg tcggcggagg
    26221 acgtctcatc ggcgaggcct gtcattttgt cgattacatg cagtcggtat gcggcgcccg
    26281 tcccaactcc gtctttgcac gacggatcgg ccgccagagc tcgggcatca ctgatgatca
    26341 atgcatcatc acgctcacgt tcgaagacgg ctcaatcgga tccatcatct acaccgcagg
    26401 cgggaattcc ggcctggcca aagaacgctt cgaggcacat gcggacggta aatcgttgac
    26461 catggatgac tttgaggaaa cgcacctcta ttcaggttct catcgcgagg tcttcaaaac
    26521 atccaaacga gacaaagggt tcaaaactga gatcgctcac tttgttcaaa ccgtggagca
    26581 ggggattcct tgcgtgatgt cgtttggaga gatcgccgcc gtaacccgag cctgcctgct
    26641 tgctgtcagg agtctgcaat ccggctctgc acacgatatc gcatatgttt agtatcgatt
    26701 tctccgtcaa tacgaaggtg cttccgaaag gcttggcctg gtatgccaaa cgtctcagcg
    26761 tcatgggacc cggtgaaatc atgcatcgca tgcgcgaagg catcgcgatg caatcactcc
    26821 aattgcagta ccggctctcc aagcgcgcca ccgctcaaca ctatgggccg aaggatatac
    26881 cacagcatgt attttgctcg gggcaagacc aacgacttcc ctctctgcct tggaaattcc
    26941 atctcgctca ggaagaaatt gcctgccttt tgaatggcac actgtcggcc ttgagccatg
    27001 agtggacatg gcgggccgat gcgaaggtct ggcatgaagc gccggatacc aggcagctat
    27061 ggccacagat tttctttcat cgcattcctt atcgagaggg caatccgttc ggggatattc
    27121 gtattgcctg ggaaccctcc cgtcttcaac atctcctcac gttggccttg tatgcccagc
    27181 aggcagagcc ggacaaacgt cgacaggctg tcgcgatgat cgagggacaa ctcctgtcct
    27241 ggttcgccgc gaacccacca ttgacaggga ttcattacat ctcagtcatg gaatgcgcac
    27301 ttcgcctcat ggcggtgtgc cacacgctag acctggtcag gccatggctg tcacagccag
    27361 aagcgatctg ggccggaacc ttgaacctcg tctccaccca cgcggagctc atccgcaaac
    27421 gcgtgtccgg acattcctcc tccggcaatc atacgattgc cgaagcggcc ggcctcgtat
    27481 acgcagggct tctcttcccc aaaatggaga tggctgaacg ctggttagcc tatggactct
    27541 atctcttaga gcaagaggct cctcaccaaa ttagacagga tggaggaggg cgtgaagagg
    27601 cgatctggta cctccggttt atctccgatt tgtacggact caccgtcgcc ctgttacgcc
    27661 agcatgaaca actcattcct caaaaactga tccaggccac tgaacgaagc catacgtttc
    27721 ttcaggcgat gatgcgtccg ggcgatggcg ccctgccacg tatcggggac gcggacagtg
    27781 ggtatgcgct ctctccattc ctccagttcg tgccggtttc tgccgaggca gcccccggct
    27841 tgacctcgtt tcatctgtcc ggctgttcaa ttctgcaggg cagcaaaggc gagcgactgc
    27901 tgttcgacca cggggaatta ggcatggcac cctgttatgc gcatggccat gccgatgcct
    27961 tgtcggtgca attggagatc ggacaacagg aaattctgat cgatcccggc acctacagtt
    28021 acaccggcca ttccgagtgg cggcgttatt ttcgaagcac tcgcgcccat aatacggtca
    28081 ccgtcgatgg ccgggatcaa gccacgcagg aaacgtcttt tctctggtca cagcctttcg
    28141 aaacccattt aatctatcgt gacgaaacac ctgaaggaaa aattacggtg ctggcgcgac
    28201 attatggcta cacgcagcca tgcggggtca cccactggcg aggagtggtc tatcagccac
    28261 ctggcgcatg gctcatttgg gattggctaa ccggaaccgg cactcaccac ttggaattga
    28321 attggcatct gggagtgtct cccgttcaaa actccgcgtc ctacgtcctg gcatgccggg
    28381 agcaaccggt tcaaatctcc gtcgaaggag gactcgcctc gctccatcac ggcgaaaccg
    28441 ctccgccttc gggatggcgc tcttcgactt atggatcaaa ggagcccatt accacactac
    28501 gagctgaata tcgcggtgcg cttccgcatg agttcctgac aagaatttgg tgcggggagg
    28561 ggccaccccc gacgcagctg caaacttttt cgttgtccag aagcttccat ggttccaaca
    28621 cgcgttgaca ttttaaatat tccagtcgat tgcgtcacga tggaccaggc tgtggcgtgg
    28681 gccgactctc gcatgacatt gtcggaaccc aagacggtga ttgcggtcaa tcctgagaag
    28741 atcatgaaag cccagagtga tcccacgctt cggcaatttt tacgctctgc gggactcttg
    28801 attcccgatg gaatcggggt cgtcatcgcc accaaactcc tcgggttagg ttatgccgaa
    28861 cgagtgccag ggtccgaact gatgccacgc ctctgcggtt tggctgcgga gaagggatat
    28921 agcatctttc tgttcggtgc cagtcctgaa gtgaacgaac gggctgcaca caaacttcgg
    28981 caggaattcc cgggactcac gattgctgga acgcagcacg gatatgttga cgacgcagac
    29041 atgccggcgg tcatcgatca tattaatgcc gtcgctccgc atttcctctt tatcgcgctc
    29101 ggcagtccca agcaggaact gtggatgtcc acgtatcttc ctcgcttgcg cgtgaaggtc
    29161 tgccaaggcg ttgggggaac cttcgatgtc ctcgccggcc gggtcaaacg cgctccatgg
    29221 atttttcggt gcattcatct tgaatggttc tatcgattgc tgtcacaacc cagccgcctt
    29281 gtacggcaga ccgctttacc tcttttcgcg tttcacattc tgcgaaaacg agtattagga
    29341 tgagatccac tcccgagctt gcgacgaaaa cggctcgctt cttggcagag ccggttgcct
    29401 ccgtctccgc agcagcaccg acccgctcac ttgcgttggg cctcacggtg cttctgcccg
    29461 tgaccatact cggcctagtc ttcacagaca gcgtggccta catggctgat ttgtggctca
    29521 ccgacgaaaa ttacgggcac ggaatgctgg tgcctctgat cagcctgtac cttatctggc
    29581 aacaacgacg acgcctcatg gaacagagcc ggtccggatc gtggtggggt ctcatctgga
    29641 ttctgctcgg agccgccctg tatgtcgtgg gtgaactcgc caccgtctat cagtttctgc
    29701 acgtcgcatt gtggtgcatc attgtggggt tgttagtgtc tgcattaggg gctcgggccg
    29761 tctccacgat tgcctttcct ttgggatttc ttctcaccgt cgtccccttg ccacagttcc
    29821 tctatcaagg gctctcgggg aaattgcagc tcatctcatc cgcgctcggc gtcgggtgcc
    29881 tccaatttat cggcatcacg gcctttcggg acgggaatgt gattgatttg gggcccattc
    29941 agctccaggt agtcgatgcc tgcagcggcc tgcgatacct tttcccgctg gcgaccctgg
    30001 cactgctctg cagttatctc ttccgggaac gcttgtggaa acgcatcgtg ctgttcatat
    30061 cgagcattcc catttccatt cttctcaacg gattccgcat cgggatgatc ggtgtgctgg
    30121 tcgaacattt cggccagggt gcggcagaag ggttctatca cgcatttgaa ggctgggtct
    30181 tattcatggc gagtctgggc ttgctcctcg ccgaaatgtt tgtcctacgc aaaatcggcg
    30241 cgacgcgtgt cacacgagac acaccttctc aacctcaacc tgaaatggtg cctcctgcat
    30301 cactgccttc tagagccata ctgctccgcc caacgtatct gtatagcgtc ggcctggtat
    30361 gcctgctgcc gttcgccgcc acagaattga ctgagcgggg agacgtgccg cccgttcggc
    30421 agccgttcct ggattttccg atgcagttca aaggatggtc cggtaccccg tttcccctcg
    30481 aagggcgcta tgtcgaggtg ctgaagtttg acgactacgt attggccgac tatcagcaac
    30541 ctcaatcagc ggcggtcaat ttgtacattg cttactatcg gtctcaacgc aaaggacagt
    30601 ccgctcattc accgcagagt tgcattcccg ggggagggtg ggaaatcggc acgattgaga
    30661 cggtgcagct tgactcggcc gcagggcagg cacccgccaa tcgtgtttcg atccagaaag
    30721 gtgatcagcg ccaactggtg ctgtattggt ttaaacaacg ccatcgcatc gtggcgaacg
    30781 aatacctggt gaaactgtac gtcctgtggg atgccatgac acgacagcgg accgacggcg
    30841 cattgatacg attgaccacc ccgatccaac agaatgaaag cgagggcgcg gcagaacagc
    30901 ggctcctgca cttcgctcaa gacatgaatc cgatcttgac ccggtatgtt cccgactaga
    30961 ctgagcagat agcacagcat gatttccaca ttatttttca gctttgtcgg ttcgctgatc
    31021 atctgcatgg cgctcatccc gccattgatg gcgactgcca gccgcctcca atttctggat
    31081 attccgaaag atcagcgccg ggtgcatgcc atccccgtgg ccaaggttgg gggaatcgcc
    31141 ttcggggtcg gcacgttttc cgcaatgctg atctgggcgc caaaggacga cattgtggtg
    31201 gccggacttc tcggcggagt caccattctg ctgtttggta tgtgggatga ccgggttggc
    31261 ttgccgtata cgatcaaatt tcttgggcaa actctcggcg cgctcatcat catgtggcag
    31321 ggcaatattc acctcaactc gctgccattt gtcgaaacgg aactccccct gtgggtcgcc
    31381 tatcccttga cgacgctgat cattgtcggg gtgacgaacg ccgtgaatct tgccgatgga
    31441 ttggacgggc tcgccggcgg cttgtcgttg ttgagcttcg caggcatggc ctgcctagcg
    31501 tacctgggag gagatcaaac cgtcacactg ctcatggttc cggtcctggg aggattgctg
    31561 ggatttttac gcttcaatac ctacccggcg cggatcttca tgggagatgc cggaagccag
    31621 tttttgggat tttttatggc ggtctctgcg atcgtcttga ccgatccgca acgcggtccc
    31681 tatacgccag ccctgggcct gttccttctt ggccttccgc tactcgacac cgtcggagtc
    31741 atgttgcaac ggctgaagga aggacggtcg ccgtttgtcg cggacaaaaa tcatttgcac
    31801 cacaaattgc tcgccatggg gctctcccac tatgaggccg tcctgatgat ttatcttctg
    31861 caggcggctc tcgtcacgct ggcctatatt ctgcgctggc agagtgaatt ggccatccta
    31921 agcacctatg gcctcgtggc gaccatgatt ttgtatccgt tcttacgctc ccgctcctgg
    31981 aagattcaat cgaatcggat gctgaagacg gccgaccgtc tcctgactcg agccagcacc
    32041 attacctctt cgccctggct gacgactgaa ccgatccaga ttctcggaat tgccgtgccg
    32101 gcgtttttaa ttctcagcgt ctttttgccc aaacacgttc cggacgatat gggctatgtc
    32161 gccgcggtga tgtttgccgt gatgctgatc ggactgtggg cctctcccag gatttcatcg
    32221 ctggtcgtcc ggggcggact ctatgtcgga agtacgttca ttatgtatct cagtgagcag
    32281 cccgcttctt ccacggacat tctcagcccc atccatatct tctttgtggg cctcgccgtg
    32341 ctggtggctc tcaccatccg tttcaactct gaacatcggt ttcaaacgac gcccttagac
    32401 tatctaatgg tgtttctcgc gatcatggtc cccgcattgc cggaaatgca gatgggcgac
    32461 atacatttga gcttcctgac ggcaaaattg atcgtactat tcttttcttt tgaactcctc
    32521 ctccacgtat tcgcccgccg gctgactcaa ctggcattgg tcgctctctg gctgctgtgc
    32581 ggcatcgccg tgcgagcctg gatataacaa ggaagcaaga cactatggct acgacatggc
    32641 atcgatgtca cacaatcgcg agattcggct tggccatagt gctgattctt gcggttggct
    32701 gtggagggcc cgaagaacgc aaagcgaagt accgcaccaa agcgcaagaa tatctccagg
    32761 cagggaattt tccgaaagcc cgcgttgcac ttcggaatgt cttaaaaatc gatccaaaag
    32821 attcggaagc ctattttctc tatgcgcagg tcgaggagaa ggagaagaac tggcgaaacg
    32881 cgtttgcgca ctaccagcgg gtgattgagc ttgagccgga tcacgagcgt gcactcgtca
    32941 aattaggcaa attttatttg caaggccgct tggatgagaa ggcacatgaa acggccgatc
    33001 acctgcttcg ggtataccca ggacacgtct ccgcgcaagc catcaaagca gcgcttcttg
    33061 ccgctgacgg caaacttacc caagcggtgg ttgccggcga ggctctcttt aagcaatttc
    33121 caaccgaacc ggatgcagcc atcctgttga gcacattgta tgcgcttcag aactcgcctc
    33181 agcgggtcga agccgttctc cgaaaagctg tggcagccca tccgcatgac cttgatctgc
    33241 tgaattcgtt agccatggcg tatatgcgtg caggcaatca ggcacaagcg gaatcggtgt
    33301 tccgtgacat tctggctctc gaacccagcg tcttcgatca tcacctcaaa ctcgcactct
    33361 ttttcgacca gcaacggcaa tatgacaagg ccgaagcgat cttgcgcgaa gcgctgcgtc
    33421 tcgatcagga caacgaacag cgacatctcg cgctcgccga ttatctggca ggtcgaaaaa
    33481 atgtggagac agcggaagcc gctctcaacg aagcccatcg ggtacttcct catgcggtga
    33541 atattcaatt tgcactggga cgtttgtatg aattgcatca actgcctgac aaggcacgcg
    33601 cgacctacga ggacatccgc gatcacaatc ggtctaaacc ggcgggaggg aatgcaaaga
    33661 taaagctggc cacgctcgat tgggctgcgg gaaaacagga cgacgccatg caacagctgc
    33721 gtgaagtgct tcgcgataat ccgcgtgcgt cggatgcgct catgctagaa gggcggattg
    33781 cgcttcaaca tggttccgct cgagaggcgg ttcaagcctt tcgcaccgtg ctcaaagacc
    33841 agccggaaca atcagaagct tacgcgttgc taggacaggc tcatttaatg ttggaggaaa
    33901 caagcttagc ccgtgagaac cttgaaagag ccgtaacact cagtccgaat ctctatcagg
    33961 cgcacctggc actggccatt cttgatgcct cgtccggacg gacaagggac gcccgagtgc
    34021 gcctggaaga attgctcaaa caggcgccga cgaatctcgg aacattgggc atgctgttga
    34081 atctgcaggc agccgaccgt gattgggatg gatccgacca gaccttatca cgcattcgcg
    34141 aggccggtgc caaccggttt gtagcggatc tcgccgaggg caatctatat caggcccgct
    34201 tgcaatggga taaagctgtg gcggcctttg aacgggcttc ttccctgaat cctgacgcgc
    34261 cggaaccgat ctttgcattg gtccgcatcg atgcacggtt aggcaagttg gctcaagctc
    34321 acaatcgcct cagtggcctc atcgcaaaaa acgcacagca tccattcgcg catggcctac
    34381 tgggagaaat tcttatcctc aaaggcgatc cgatgcgggc ggaacaagaa tttcggcttg
    34441 ccactaccct caagccggag tggggcactc cttgggtcaa ctgggtcacg ctcaaactct
    34501 ctcagaggaa acacgaggaa gcaggcgaca ttcttcaacg cggattgcag atcaaccccg
    34561 ccagcgagga actccgcctc ctctatgcct cgcacctggg tgagcgtggg cagttcgatc
    34621 aggccatcct ggagtatgaa acgattctga aacacaaccc caggcaactg atggcggcca
    34681 acaacctcgc ttcgattctg gccgatcaga aaggtgatgc gaagagcctg gatcgggcgc
    34741 tggcgttgag ccgcgagttt gagcagcgcg caccaaatcc attttttctc gacacactgg
    34801 ggtgggtcca cctaaaaatg ggacatcagg atgatgccat ccgtgtgatt caacgtgcgg
    34861 ttacagaggc cccgcaacat cccattctca attatcactt gggcatggct cacttcaaag
    34921 ccggccaccg ggtggaagcc caaacccatc tcaaaaaagc actcaacacg aaacaaccgt
    34981 ttcccggcat ggacgaagcc aaatcggtcc ttgcggaagt tacaggatag gaggaaagag
    35041 ggtgaagatt gccggttcgt tactgatcaa gaaactagcc cgaggttgcc agccaggttt
    35101 gcttgcgctc tacgctgttg tgctgatgac tggctgtgtc acgcccgaac acactgccgc
    35161 tcaaaacatg gccgatccgg gataccatct cgggccggaa gatgtcgtca tgatctccgt
    35221 gtggaaagac gaaaatctca cacgagaggc gatcgttcgt ccagatggca tggtgtcgtt
    35281 tccgctcatc ggggatgtgc cggctatggg acataccgtt gaagaggtgc gtcaggacat
    35341 tgccaaacgc ttagcccggt ttattcctaa tccgcatgtc tccgtggcaa tcacaaaact
    35401 ccagagctac aaagtgtatg tcatgggtcg ggtcacgaag ccgggcgaat acctcgtggg
    35461 acactatacc gacgttctac aggccctgag ccttgccggc ggtcttacgc cgtttgcatc
    35521 ggagaatgac attaagatta tccgccggga aaacggtgac caacgcgtct tcctgttccg
    35581 atacggcgac gttcaaaaag gcagagacct caatcaaaac atcctccttg agcgggggga
    35641 tgtggtgatg gtgccgtgat gcatcatcga aactcgatcg gcatcgcgaa cgtgtcgcgg
    35701 tgaagcatcg tcttattcca acagcctggt tatgggccat tcttttgacg accgcgtggt
    35761 cgagtcatag cctcgatcag gaggtctggt ctgcagaatg gtttttggaa ccggccttga
    35821 cggtgcgcgg ggaggtcaat agtaacctga ctctcaccac gttcgaccat aagacgacgt
    35881 atggtcattg ggtatccccc ggtgccaagt tcgggggctc aacggaagcg ctggaggtca
    35941 acggacgatt ggcggcggat tttgttcgat actatggagg gcagaatacc agccttacga
    36001 atctgtattt cccgctttcc gccaagtaca aaatggagcg tgacagcttc ggttttgatg
    36061 gcggattcac acgcgataat accctcatgg gcgaactgcg acagacaggc gtggtgctca
    36121 gtttcactca gcggaatcag tggatactca acccgtcatg gacgcatgcc attaccgaac
    36181 ggctctccgc aacgggctct tacacgtttt acgacacgcg ctatgagaac ggcctgcgcc
    36241 tcggattggc agactatacc ttgcatgttg ggtcgacggg catgacatac caggttggtg
    36301 agcgcgatca ggtacagctg aatgctgtgg gcgtgcgttt caatgccgca gatcttcggc
    36361 ttagttcaac gattatcggc cctcagatca cctattcaca catgttttct gaaaccttga
    36421 cgggaactgt atcgggagga ccaaggcatg tgtcgtccac catcaacact tccgccgggt
    36481 ctttatccga tggtgtgtgg acctgggtct tttcagggaa cttggaaaga cgatgggaag
    36541 actggcgggt gaaaggtgag atcggaagag aaattaaccc gagcggattc ggcctgctgc
    36601 tccgtaccga taaactaagc ctggaagtgt cccatcaaat gacggatcgc ctgtcggctt
    36661 cggtcaccgg cttgtttctc atcgccgatg gggtgaaaac cagggccgtt tccaccgtgt
    36721 tccctgagaa ccggctcttt tatgtgacac cgaagctcag ctggcggctg tcggaatggt
    36781 ggacggcgga cattacctat acgtatgccc aacgagacct tcccagccaa tcgttgaccg
    36841 cactgggcaa ttctgcaact attatgttta cgtattaccc tccaaggttt acggtaggac
    36901 ggtagagaga tccactggat cggaaagtgg caggggacca tgaataagcc aacggtcggc
    36961 atccaagaca tcatttcgat ggcgaagcga cgtaagatgc ccatcatcat gatcaccctc
    37021 ggcattctcc tcctgggtgc caccctcgca ttcaccattc cagcggtcta tcgctccacg
    37081 gcgaccatcc tgattgaaga acaggaaatc ccctcggatc tcgtgcgttc cgccattact
    37141 acgtacgcgg atcaacgaat cgaaaccatt aagcaacaag tcatgagccg cagcacattg
    37201 tggaaaatcg tggagcagta cgacttgtac aaacacatgc ggaagcagag tccgaccgaa
    37261 gaagtgcttc atcggtttac aaaagatacg aatatcgatg tcatcaatgc gaaagtcgtc
    37321 gacaaacgga ctcagaatcc cacccaagcc accatcgctt tcacactatc gtatgacgga
    37381 gagagtcctg aatcagcgca aaaggtcgcc aatgaactga ccagtttatt tctggcggaa
    37441 aacctgaaaa ctcgcgaacg gcatgcccag gaaacgaccg ccttcctcaa gcaggaagcc
    37501 gatggtctct cccgtcacat tgaggagatt gaaaagaaac tctctatcgt caaacagcgt
    37561 gcagatggcg cacttcctga actgacacaa ttaaatatgc aactcttgaa tggtgccgag
    37621 cgcgaactga ccgatactga tcgggacatt cgcagtttgg aggagcgaaa gacgtttctc
    37681 gaaggtgaac tcgccaccat taaacctaac acgccgattc tctcgtcgac tggagaaaga
    37741 attttggact cgggcgagcg gctgaaagcc ttgcgcgcgg aatatgctga taaagcgggg
    37801 tatctctcgc ctgagcatcc ggatatttgg aaaatgcagc aagagattcg agcgctggag
    37861 agagagacgg gcggagcggc tcccatcgac gatgtgctga aacgtctcac cggtgaacga
    37921 gctaccttag caactctact cgacaaatac ggggaggatc acccggacgt aacgaggtcc
    37981 agacggacca tttcctcttt ggagaaagac cttcgggaat atgaaaatgc accggccaag
    38041 atgccagcca tcaaaccgga gaaccccgcg tacatcaaca cccaaacgca attagcttcg
    38101 gccacttcga tgttggaagc gctgaaagcg caacgagcca aattgcggaa acgtgcggaa
    38161 gactatgccc ggcgtctcga aaaaactcac catttcgaac cggagtactt agacctcgcg
    38221 cgggagcggg acaactccgc ccggaaatat gaagaattgc gttccaaact tcttgaggca
    38281 caagtttccg aagggctcga gacacagcgc aaaggtgaac ggttttccct gattgatccg
    38341 ccggaccttc ccgaaaaacc ggaaaagccg aatcgtccac ttatcctgct cctgagtttt
    38401 gtgctggctt tggcaggagg agcgggcagc ggggtcttac tggaaaacgt cgaccgttca
    38461 atccgcggcc cggaatcact ggctcatatc acccagacct atccacttgc cgtgattccc
    38521 tacattccca ataacgcgga ccttcgccaa ctgattgcca agcgtcgaac catccgcctt
    38581 gccggcatcg gcatcgcggc tgcctgcatt ttattggcac atttcttctg gctaccgctc
    38641 gatgtggtgt ggttcgccgc gctacgtaaa ttcggtctcg attaaccgag taggagattt
    38701 cacatggaac cgctacacag tgcaatggaa cgattcaagc gggaggaaca gcgacgcccc
    38761 acgccggtcg ttctcaaagc accgccccct cgaatcggaa aagatgctgt gcaggatatt
    38821 gtgtatacgc acacccgcac cgtcgacatc ccggacgacg tcctgcgtga acgacgagtc
    38881 cttgccggtt tcgagcgcgg cccctttctt gatgcgtata aaattctccg cacccaagtc
    38941 atgcaccgac tgcgcgaaca tggttggaac gtgctgggca tcaccagccc gagcaaacaa
    39001 gaaggcaaaa cgctgactgc ggtgaatctg gccatcagcc tcgccatgga tacgacacaa
    39061 acggtcctgt tggtagatgc cgacctgacc aatcccagcg ctcatctggc atttggaatt
    39121 gaggagtgtg gagggctggt cgattatttg ctggatggaa tacccgttga gcacctgttg
    39181 attcatcccg gaatcggacg gtttgtgctt atgccggccg gccgatctat tacgaattca
    39241 acggaagcat tgacctcgcc caagatggcc tcgctcgtcg aggagttcaa gcaccggtat
    39301 ccatcccgca tcgtactgtt tgatttgcca ccgctgctgc agaccgccga tgtgttggcc
    39361 ttttcgccct acaccgacgc cttgctcctt gtcgtcgagg acgggaagac cacccgtgaa
    39421 gaggtcgagc gagccctgca attagtcaaa ggcagcacac cggtattggg caccgtgctt
    39481 aacaaagccg gcaaaggcca catgaccccg gcggcaatga agcgaatgtt attgacctaa
    39541 acaccttact ctcgtaaaga agatccatgt acgaacgctt ctatcacctg acggtcaagc
    39601 cttttactct cgtcccgaat cccgattttc tctatcgcgg ggagacccat cgcctcgcgc
    39661 tcaccaccct cgaatacggc atcctcaatc acgcggggtt cgtggtgctc accggagagc
    39721 ccgggatggg gaagaccaca cttattcagc aaatcctggc cgaacatcgg aaccatttca
    39781 ccacggggct catcaccaat acgcatgagg gcatggaaac tctcttgccg tggattttac
    39841 tcgccttcgg ccagaacgat aaaaacctgg acaagctgga agggtttcat gcgttccagc
    39901 aattcctcgg caaagaagcg gcggctaaac gccgggtctt gttgatcgtc gatgaggcgc
    39961 agaatctcgg agcaaatcta ctggaagagt tgcggctgct ctccaatctc aacaaggata
    40021 aggcgcccat cctccagatc attttgtcag gacaaccgga ccttcgacag ctgctgcaac
    40081 ggaaagatct gacccaattc gcgcaacgtg tgatggtgga ttacagtctt gaaccgttca
    40141 aagaagaaga tagcatcgga tacattgccc atcgtttgcg agtcgcgggg cgagaagagc
    40201 cgctcttcac acatgaggcc gcgcggacga ttcatcggct gaccggcggc attcctcgct
    40261 tgatcaacca actgtgcgat acggccctga cgtatggctt cgccgaggaa gcgccatttc
    40321 tgtcggcgcg gatcgtggcg caagccgcga aagaccggac caaagggggc attcttccgc
    40381 tgtcgaacac tgaatccctc acggcagtga cgcctgatca acaggctgcc gaacaaaccg
    40441 aactggcggc actgtcagct cgagccgcat ccacgtccaa tgaagttccg cgccgtgatg
    40501 atgcagtccc gacttcttca ggtgtggcta cagcacgacc agacaccaat gaacagtacg
    40561 agcggggact gtcgctcaag aaagccggat cgtataagga agccttacaa gcgttttctc
    40621 tcgccgccca ggatgccgcc ttcaccttta aagcaatggc gcaggttggg atttgcttga
    40681 aatccctcgc gcaaccggaa gaggccgtta cggcatttcg aaagtcatta caggcaaagc
    40741 gctcggcatc ccctgatact atccaggttc ggtatctgtt ggcgcgcacc cttgagtcgt
    40801 tagagcgcac agatgatgcg cttgagcaat accgctggat cggccgagaa gagcccagct
    40861 tcaaggacgt ggcggaacgg ctcgagcggc tccacggccg ccccatccga tccactcgca
    40921 atgaagcggc aagcagcgac ggatcctcct gggtgaccca actgcagcgt attctcggcg
    40981 cctccaagta gccagcaaca ctcctgacat ttctgcctgc taccaccgcg ttcaagtttc
    41041 attcggtacc aaccgatgat aagtgcaggg gagtgtccgc atggctttag aaatcattga
    41101 aattgtcgaa gatgaccaga gtcaggctaa attattggat caaatcctcc ggcaggcgtc
    41161 ataccgcacc aacgtggcat tcgatgggcc ggccggtatg caggatgtct ggcggatcaa
    41221 accctcctta atcgtggccg atgacaacct tcccggattg accggccggg agatgtgcaa
    41281 gcgcttacgg caggaccctt ccaccacaca cattcccatc attgtcatgt ccgggttttc
    41341 gtccgaagag cgccgggcag aagccctcga cgcaggggcc gacgacttca tcgtcaagcc
    41401 ctattccggg atggaactgc tcgctcgcgt acgcgcactg ttgcgtcgtt gccggcaaag
    41461 ccaggcgcag gatgaagaac tccctgaaga tctcgccttg actgaaaatt tgtatgttgc
    41521 ggtctatcga ggaaagagcc tgacgttatc cgcgcacgag tggaaagcgc tgcgtcgatt
    41581 ggcgagtacc gtcgggaacg ttgttccgcg cgaggaactc aaatctttgc tctggggaga
    41641 cgacagcctc atgcacgacg gagaactcga tcgatgcatg caacagctca accgaaagct
    41701 gtcgcaagat gccccatcgg tggagtccat caaaatcgtg ccgggaggtt ttcgcctctc
    41761 gacaccagcc tcggacgact caggcggacc tgcggttcac tgatgccacg tgaagatttt
    41821 cgccgctcca cacatgcccg gatgccaacg catccagtaa ccacgacatc gttccgggga
    41881 catgccgggc tcgttttctc gctcgcttcc agtccttctt catgcgcttc cgcaccggaa
    41941 gactcagctt cttaccccaa cgatgaaaat ctgccagctc ctcatatttt cccaataaag
    42001 attgaacccg cttgagttgg gaaagcatct cttctttcgc tgctgttcga ccgggcaacc
    42061 attcagtctg ataccggatc gtcttcaagg cgagacgcaa cgcgtggagg cgtttccgcc
    42121 gaggcttgtc cgatgcagcc gcgatgagcc gtttgagcgc gcattcatgc tcctgacgca
    42181 gttcatcaag ccggcgattc caggactgag gaggggagag gatcgcggga agcgcctgct
    42241 tcgagacaac ctgttcgatc ctgcgatagg cctccgtgtg attcaacttc ctgatctgtt
    42301 tcgtcagccg gtcatccacc acggccacat cttttcgccc ggcgtgacac ctgatcacgt
    42361 actgcctgaa cacctggagc gctcgcagtt tgctcaaccg cctcacacaa gctgccactg
    42421 cacgagcacg tttcgcctcg ccgtagagtt ccagcaaagc ttgaagtctt cggcaatgcg
    42481 tacggatggc gtgtatcgtg tccacatgtt ctcttcccgc aatcgcttgg cgcaacaaat
    42541 ggagaacggt ggcgtgactg gaagccgcca tcgtgagcaa cccttgagtc ggtgactgcg
    42601 gtcctgtatg aatagaatgg agcatgtcac ccatgacgaa taatcaattc ctgtgcgatg
    42661 gggctcacgg aagatcaccc attgcatgaa ttatggccgg cccgaccacc ttgcgtccat
    42721 cccaagggag tacgttgatg gattgtctct tatttcgaca cggcattgcc tacgatcgtg
    42781 aggactggcg ggggaacgag cacaaccggc cactcacgga taaaggcctg gttcggacgc
    42841 ggcaatcaag taaaggattg ctgactctag gctgtatccc gacgcttatc ctttcaagcc
    42901 ccctgacacg cgcccagcaa acggccaaga ttgcgagatc gttgattcct gcaacgccgc
    42961 cgctgcggat ctgccaggaa ctcaatccag aagtccctcc gcgtgcattg attgcactgc
    43021 tcaacacact cccgtccgac gccgtggtcc tctgcgtggg gcacgaacca cacctgagcg
    43081 cgacggctgg aatcctcctc acgggaaagc tgtgcaaagg gctttcgctc aaaaaagcag
    43141 gcgcgtgcct acttcatctt gaagggtccg tgcgtcccgc aaaaggccgc ctggagtggt
    43201 ggctcaccgc gtcacaattg cgcgcactgg cgtaaggtgc tcgcgcaagc gtcgccattc
    43261 gatgattcac tgctgagcgc actcgcttag aatacacgcg gcttcgaaag taagttcgtg
    43321 cagtaaggag tgcctgtagg agttgaccgt ggatcccgat tcaggtggca tcgtcgtctc
    43381 gggtggtcgt tatgaactgc accggccgct tgaagacttt ctcaaacaga tcgctccggc
    43441 cgcgcgccgc ccaaatctct aactccgcat cacccgacac ctgcgcagta atcaccacgg
    43501 tcttgccgag tttgacgtcg atattctgca ccaccgaaaa ctggcttcgg tctaagccat
    43561 cggcaatgcg cagcaatgcc gacaagacat tgatgactcg ctgggagccc tccggcaata
    43621 ccgtaaattc gtcatgcttc ctggtaggaa cggaacggcg atgatagcgg gcgatattcg
    43681 caatcaaatc gatttcctca gcggtaaacc ccgagagatc gctattcttg atcaggtagt
    43741 aggcatgctt gtgatgctgt cgcgaattga tcatatagcc gatatcatgc agaatcgcgg
    43801 caccttccaa ccactcgcgt tcccgctgtc cgagtccatg taacgccttc gtctgatcaa
    43861 atagacgtaa ggccaatccc gccacatgca acgcatgcgt ttcggagaca tggcatcgat
    43921 gggcgagggc caggatgttg cgtttacgca cgtcgggaat gtctcgctcc gcttggatac
    43981 ggtcgtggtg ccgctgaatg aaatcgtaaa taatcccttc gcggatcgct ttgtcgcaga
    44041 gggtgagctc cgttttctgc aacagctcca gcaagatcct aaacactatg gcggccggaa
    44101 gcagggtgtc cacacgtttg ggatccagcc ccggcatggc cagacgcgtt ttcaacgaag
    44161 cgccggcaag tcgtttttca acggcggcga tgtccttggc ggataccctg gccagattga
    44221 gctgcggaag cggccggccg gtgcgttgca gatgaatcac ctcagccagg ttgcccgcca
    44281 tgccggacgt cgcgataatc tgctccaccc gtttggtttt gtacgagccc aaggcattct
    44341 tgagttgagc ggtcaccgcc gcttccagat cccgaagcat cgcttttgat ggaggcgttt
    44401 tagagaggta gagatccttt aagcgaattg cgcccagctt caggctccgg gcctgaaaaa
    44461 tggcctcgcg attcccgacg atgacctcca ccgacccccc gccgatatcg atcaccagcg
    44521 tgggcgactc gggcagggcc acgctgttct gcacgccgag gaaaatcaac cgggcttctt
    44581 ccgcgccggt aatcactcgc acagtcaggc cggtttgctg catgacatga tctaggaatt
    44641 cgccgccgtt cctcgcctcc cgcacggcgc tggtggctac accttcgatc tgttcgtacc
    44701 ctttattgcg ggcaagattc acgagtgtgc ggatcacttc caatccgcgc atcatggcct
    44761 ggtcggaaag acgccgggag gtgaacaccc cgtcgcccag acgcgtcatg tctttgaaac
    44821 gatcgaggat tttataggag taatccggct gaacttcggc caacaccata tggatggaat
    44881 tggtgccgat gtcgaggacg gccagtttgg gcatctagaa actcgcggta ggagcgacgt
    44941 gccggatgtt cttgccttca accatataga cgacaagttc cgcgatgtta gtggcatgat
    45001 cggcaattcg ctcgagcgat tttgccacaa acgtcagacg gatcgcgcgt gtaatggtac
    45061 gcgtgtcctc cagcataaag gagagcaact ctcgaaacaa ctgttcgttc acctcatcga
    45121 caaaatcgtc gtccgtgcag actttcctcg ccaagaccgg atcggaattc acgaaggcat
    45181 ccaggcactc cttaaccatg cgcatggtcc aattcgccat gcgcggaatg tcgatgtatg
    45241 gcttgagctg cggttcgctg ttcagctcca acgcccgctg cgcgatattg tcggccaggt
    45301 cacccatccg ttccagctcg gaagaaatct tcatgccggt ggtgacgaac ctgaggtcac
    45361 gggccgtggg ctgttgaagg gctaacaact gtacgcagag ttcgtcgatc tcgacatcca
    45421 atccgttcac gtcgtgatcc tgcttgatga catcgacggc gagatcggaa tctcggtcca
    45481 ccagggcctg caaggcctgc tggatttgcg actcgaccag gccgcccata cggagcagct
    45541 gctgtttgag atgcgcgagg tcttgatcga agtgtcgttg catagttcag gctcctctcg
    45601 cgatttatcc gaatcgtccc gtgatgtagt cttcggttcg tttgtcacga ggcgtggtga
    45661 aaatggtttt ggtgtcaccg aactcgacta actcgcccat cagaaaaaat ccgcactggt
    45721 cagagacccg tgccgcctgt tgcatattat gcgtcacgat gacgacggtg taggtctcct
    45781 tcaagctata gatgagctct tcgattttcc ctgtcgcgat aggatccagc gcggagcagg
    45841 gctcatccat gaggatcact tccggttgca ccgccagggc acgggcgata cacagccgtt
    45901 gctgctggcc gcctgaaagt cccacggcac tgtgctgaag gcgatccttc acctcatccc
    45961 aaaggcctgc gccttgcaaa ctgtgttgga cccgttcgtc gagcgaccgt ttatctttga
    46021 tcccatgcag acgcggaccg taggccacgt tgtcgtagat ggatttggga aagggattgg
    46081 atttttgaaa caccatcccg acgcgcttcc gcaaatcggt gacatcgatg gccggatcgt
    46141 agatatccat gccgtccaat tccacacggc ctaccggacg ggctccctcc accagatcgt
    46201 tcatgcgatt caagcaccgg agcaacgtgg actttccaca accggaaggg ccgatgaagg
    46261 ccgtgaccga atggctccgg atcgtcaacc ccacatgaaa cagcgcctgc cgatgtccgt
    46321 aataaaaatc cagatcgcgc actgatattt ttgtctgcac cggcgacccg aaagcccgtt
    46381 gaggcgacgc gagccgggag ggggagatgg cggggcgtaa agaaaccttt ggcgccgtca
    46441 ccgtcatcct gtctgattga acttccatgg gaactcctct ctgtgatcac actgccgagg
    46501 tcgcgaatcg cttgcgtaag cggttgcgca acatcaccgc agccagattc aacagcacca
    46561 ccacaagaat cagaatgagg gtggtcatgt agaccatggg cttggccgct tcgacattcg
    46621 gcgactgaaa ccccacgtca taaatatgaa aacccaagtg catgaatttg cgttcgagat
    46681 gcaggaaggg aagatactcg tccagcggaa gcgccggggc cagcttcacc acgccggtaa
    46741 gcatcagcgg cgccacttcg cctgcggctc tggccatagc gagaatcaat cccgtcaaaa
    46801 ttccgggcaa ggcgcaaggc agaatgacat gccggatcgt ctcaaatttg gtggccccca
    46861 accccacgga tccttcccga aaatcgcgcg gcaccgcgga gaggccttct tccgtggcga
    46921 cgatcacgac aggaacggtg agcagtgcga gagtcagcga agcccagaga atcccgcccg
    46981 tcccgaacgt gggattcggc aaggattcag gaaagaggat ctgatcgata gttcccccca
    47041 gcgcatagac aaagaaggcc aacccgaaca cgccgaacac aatcgagggc actcccgcca
    47101 ggttgttcac cgcaattctg accagcctga cgagcggtcc ttgtttcgca tattctcgaa
    47161 gatacacggc cgccatcacc ccgaaaggca tcacggccaa ggtcatcaaa aacaccatca
    47221 gcaccgtccc gaagatggca ggaaagatcc cgccctccgt attggcttcg cgaggttcgc
    47281 cggagacaaa atgccaaagg ttccggacat aggccacagc cttttcgaac ccattcatcg
    47341 cattcggccg gctgacagcc aagacctgat cgagcgagag gcgcaccgat cgtccggtgg
    47401 agagggtgag gagcacgacc tcctgggttg cctctttatg caattgctcc agtgcgtcgg
    47461 tctttgaaag gtattcacgt tcaagcgcgg cgatctgttc gacgagacgc cggtctgctt
    47521 caatgctggc ggggttatgt gcacctttca acaccagagt ctggcgcgca agcctggcct
    47581 tttcgatacg atagttaatc gacccgatgt cctcccgctc gagatgttcg atccgctggc
    47641 gcaactggtt ggcgcggtcc acgagcgttt cgatggtgct ccaggcgctg ccatcagcgt
    47701 tggttgccgc agtgctttct gctgcgaggc tgatacggcc gtaggcaggg ccccattcac
    47761 gacgctccac gaacaccaga tcgtcgggcg cagatatcga ggcgatctcg gattcattga
    47821 tccaccggta ttcggaaccg aattgatccc gattgccgac gcgataacga aagcgccgtt
    47881 gccccagtag cgcatcagtc gtcatcgaat ggggaatcgt ttcctcgccg acgaggtgtc
    47941 cgatcaccgt ctcctgcgtc ttcagcgtca cctgttgcaa ggtgccgggc caaaactgcc
    48001 ccaggccgtt gaacataatg aggaccagca tcccggccac catcagcaag ctgatggcca
    48061 cgccggatgc cgtcatccag acgaagagtt ctccgcctcg ccaaaactcc ttcatgcgca
    48121 gagatggctt acccatggct atatttgtcc cggagtcgct ggcgaaccag ttcggcgagc
    48181 gaattaatga caaacgtgac aatgaacagg agtagcgcgg ccaggaacaa gacccggtac
    48241 agacttccgc cgtgcggcgc ctcggggatt tccacggcga tgttggccga caatgtccgg
    48301 aacccgttgg cccagttcca gtccaggatc ggagtatttc ctgtggccat gaggacgatc
    48361 atggtttctc caatagcgcg gccgaaaccg atcatcacgc cggaaaagat tcccgggctg
    48421 gccgataaca acaccaggtg caacacggtt tgccaacgcg tggcacccaa cgccagagac
    48481 ccggcaattt gagatttcgg cacgttggag agggcctctt cggcgatgct gtagatcacc
    48541 ggcacgatgg caaatcccat cacgatgccg acgaccaagg cgttacgctg atcatattgg
    48601 ataccgagcc gactagaaag ccaggttttg atgttgccgt caaaccacca gacttcaaaa
    48661 aatgaatggg cccacagaca cccgccgatc ccgagagcca gaatgggcat taacagcagg
    48721 gcttcattcc caggggagaa atgccgtttg atagacatgg ggcagagatg ccaagagaag
    48781 gacgccacga tgaccagcac gggcaaaacc aacaccatgc cggcaagcgc gggaagcatc
    48841 cgttcgagca ggggagctaa ccagagcccg gccagaaacc ccaggaccac ggtcggcaag
    48901 gccgccatga cttcaataat cggtttaact ttcgcgcgaa ggtcgtgatg catgaactgt
    48961 gaggtacaga tggccgcgaa aatggcgata gggacggcaa gaaacagcgc gtatatcgtt
    49021 cccttgagag tcccgaaggc caagggcagc agccccagtt tcggctctac ctcgtccgat
    49081 ccggaggacg attgccagac ataggctggt tgaccgtacc cttcgtacca aaccttgccg
    49141 aagagagtgt ccagcgtcac ttccggatag gggttttgta cctgatagag cgacagctgt
    49201 ccggctccat ccaaggcaat caccccatcg cctttgggag caaaggccac ggcacgaatc
    49261 ggaaactcgc cgttggccac tcgcaacagc gtttgcgaag aggtcgcatg gtgaaggaac
    49321 acattccctt ttgtatcgcc cgtgacaaag cctttgaccc gttgagacgg ggcaaacgcg
    49381 gtcaccggag ccgcatggga acggaaggta tgcatctgag tgagcgtcca tcccgtgggc
    49441 acatcggatc gccgcaccag gccccacgtc gacacggccc cgtcagccgc catcaccacc
    49501 agactgcgat ctccactgag gaatcccagc atactgatcg ccgtagtggg tccggcgacc
    49561 gcatacgacg ccctgacatc cggcggctgc gtctctgaca cacccacatg gatgatgtgc
    49621 ccatcggttg tgcccacata cagatgttca agcgatgctt ccagcgccag agacgcgacg
    49681 ccaccgggcg gctgcggcaa ttccgtgacg attacacctg ctcctgttgg atcgtccgga
    49741 gcaattctgg cgaggaagag acgtcccgtc ttggtagcga gcgcaaggag actgccatga
    49801 tcattggagc gataggccag acgaacaatg gggctatccg ttaccggaat gggcgcgccg
    49861 gcgcgaacgt gaggccgttt ccgccgttcg ccctgatcgg aaaactccgt cgtggtgccg
    49921 atcttcaaca gcaagatttc gccggctgcg gtccctacgg ccagacgcgc agtggggccg
    49981 ccgccggagg ccatggccgt cacttgcctc gaagtcagct gagccggcat ttccagtgcg
    50041 atgggctggc cggaggccag atcaaaaaat tgaatcgcac cggacgtgaa gacctgcgcg
    50101 atctctcgat gctcatccac cgccacctgc gcggggcctt cgtctgccag caaggccggc
    50161 acgctcaagc gttggcttaa ctttgcactc ggcgcggtga aaagcggcgt gacctcgcga
    50221 aagagaaaaa agaagatgcc gagaatgctg acgatggtcg ccagcccgcc cactgtaatg
    50281 atgccacggg cgagccggtc gatcactgac cgcaagtgga ccggcccgga gagatagctc
    50341 aaaggattcg tgatcattac cgaatcttgc cgagttgctg ttcagccaat ttcgcatcga
    50401 cagggaagta gccgtctcgt aacacgtcgg actggccttc cttgctgtat acgtaggcca
    50461 gaaactcctt gacgataggg gagagcggtt ttcccggcgc ctggttcaca taaatgtaga
    50521 ggtaccgcca caacggatag gttccgttct tcgtgttcgc ctgcgtcggc tcaatgtacg
    50581 gttgccccgc cttcgctgcg agcggcacca ttttcacccc ggacgtgcta tacccgatgc
    50641 cgctatagcc gatgccatac cggtcctcgc tgataccctg cacgacagag gcagatcccg
    50701 gctgctcctt cacttgatct ttgaagtctc cgtttttgag cgcatggtcc ttgaagaatc
    50761 cataggtccc ggacgccgag ttgcgaccgt agaggctgac cggcgcattc gcccaatcgc
    50821 cgttctggcc aagctgtccc cagcgcgtca ggtccgtggt gtgcccctgg cgtcgcgact
    50881 tggaaaacaa cgcatccact tcggcaatcg tgagtcctgc caccggattg tccttgttca
    50941 cgaagagcgc cagcgcatct accgccaccg gataggcagt gggagggtaa ccgaacttcg
    51001 attcaaaggc atcgatctcg ctcgatttca ttgttcgcga catcggcccg agctgagccg
    51061 tgttttcgat cagggcgggc ggggccgtgc tcgatccttt gccttctact tgaattttca
    51121 cgttgggata ttgtttacgg aatccctccg cccagagggt gatgagattg ttgagggtat
    51181 ccgatccgat gctgttgatc gttccagaca cgccgctgac cttatgatag ggtttgatac
    51241 cggcatcaac agtgagcgtt tcgaccgcgg gagtatcacc cggcgctgcg ctgaccaggc
    51301 acatcgtcat aaatgggcct aacgcggtca acgtgagact caagcaaatc tggcgtgaga
    51361 cggtcataga agacattcct ccggttaaaa gagccgtaaa ctattgcacc gtcgagcata
    51421 cggccgtttc atttccagac catcagccca atgtaaacac cgtgttaact ctcggtgagc
    51481 ctcgctttct gatgaaagaa tcgtgatcgg cttctcgagt taaccacacc gacatctcgc
    51541 cgtgaggaat ctgaaaccgt cgctccgtac cctcttatgt catgagaccc tcgaccgtac
    51601 atatcgttga agacgaaccg ctccacgccg gactgctcga tcgtgcacta cgccttgcgg
    51661 gctttgacac cttgctgtcc agcgacggcg ccagcgggtg gcaggagatt caacgccgcc
    51721 taccgacact cattctgctc gacctcatgt tgccggcact cagtggtcaa gagatctgcc
    51781 ggatggtgcg ccaacattcc gcaacccggc acatcccgat catcatgctg acagccgttg
    51841 gaggggaacc cgaacggatc gccggcctcg atatgggcgc agatgattat gtcgtgaagc
    51901 cattcagtcc gagagaggtc gtctcgcgcg tgcgcactgt tcttcgtcga ggtgcacact
    51961 cagcggacgg ggtcgccccc attgtgaatg cggctgtcag gatgcagggg ccgtattttg
    52021 ccgttctgtt gggggaacga gaactgctcc tgtcccgaaa ggagacggcg ctgctccaga
    52081 ccttactcgg tcgggaaaat gccttggtgg ctgcagaaga gttgttaacg cttccggaag
    52141 gggagaggcc ggggctgcta ctccaagaac tggattacga cgtccgtggt ctgcgtcgaa
    52201 aactggagaa cagcggggcc ggatccgtcg acatgttgcc gggatttcgc tatcgattca
    52261 ggtcacggct ttgacgccca tcatacgtaa gcaatccgca gatcacgcct tcacccaaag
    52321 caggacaatg caggttgacg ggaagagatc cgttctattt cgtaatcaaa ccctcttcgc
    52381 aagagaatga tcttcccatg ccggctcaac tcatcaatcg tcttgaagac ctgattccag
    52441 ctcagttcgg gaaggagtgt gacgacctgc tcaagcgtaa gcgatttcct gccatgcaat
    52501 aactccagaa ccctgcactc actgctcagc actgcagact gatgaaccat ggtggatcct
    52561 cctggttgga atgtccccaa gccttctact cgctgtccct ggcaaccggc tgactgctgg
    52621 atcacatgca gctgactgtg caacatgcgc ctctctttca actcaaccct gcatgaacat
    52681 gcgagcactt gtcacctgta cgcgaaggaa ttagattccc tcccggcagg atcctgcgca
    52741 ttttccgttg ccatcaccct gagcaggtga gcagcgcact gcgacgacac gtaccgcccc
    52801 taacgccaga acgatggcac ccaacgacag gagcttcgtt tggtcggctt ctttgaacca
    52861 cagggaaatg atctccgtca gcatgacgac caaaatcgtg tcgacaataa atgtcacttt
    52921 gacgcgtccc tccgaaaaat aggtcagcgt cgtcttaaag acttcgacca ccgccagaag
    52981 gatgagcgta tccacaataa tctgccgaag cccaacctca accggagcct caagtaacaa
    53041 tttgatatcc agaaatgtct tgatgacccc gcccgtcagg gcgatgagga tcgtcagaat
    53101 cagaagactc agcacggctt taataccctt catccagagg tcggtgagat ccgtttcgat
    53161 ggcgcgccct gcgagatctg ccacacgggg aacccgaacg gggaaactgg taaacgtcat
    53221 ctgtgaaacc tcctggtgaa tgacacgatc atggttgagc aacgtgctcg agaggtccgt
    53281 ccccggatcc ccatggacca taaccttcat cgccactgta ccgctgaatt gttccgtgat
    53341 attcacaacg gcattacagc agcgttacgt tttctagggg cgagccgcct cagcggacca
    53401 tgcggacatc tcactgcagt atttacacag ccgtaacaaa cgggatacag gcaggcaact
    53461 cgatgccgct agcatcaggc tatgcagcac gcgacggaac cgctacgtca tcacggcatt
    53521 ccggtgcttc ttgtcaccaa agacgaagaa tttgccatgt ctgtccacca gctgttacgg
    53581 ccgaacgggt atcatgtgtc tctcgcacca accgctgagg ccgccttgtc tcttgcttcc
    53641 ggtgcctcgt tcaaactggc gctcatcgac cggcgtcaca aagtcgtcgg gacgttgcgg
    53701 cagaacctcg cattccggca catcccgatg atcgcgcttc aacccctggg agaaccgtgt
    53761 acggaagagg agaccgtgga ggaccttgcc gccggattcg atatcgtcat cgactcacat
    53821 cggcatcgag aatggctggc gatcatcaag gccctgcttc gacgacagga aatacaagct
    53881 gcgcccctca gggaatttcg agtcaatacg ctatgcatga atctcgatcg acacgaggtg
    53941 agggtcgaag gcaaatccat ccagctcacg ccgaaggagt tccagattct gcgcgtgctc
    54001 ttgcagcatc cggggcgcgt gctgacgcgc caggaactac tcaaccttgt ctggggagaa
    54061 gactacgcgc tcgaagaaca tgccttggac gtgcatatcc acgcactgcg ccacaaattg
    54121 gaaactaagc cgtcggctcc gcaatggatt ctcacggtca gaggcgtggg gtacaaactg
    54181 tccacggatt gagccgttgc cataagtcgg caccgcaaca gacatcaatg acgcacgaca
    54241 cggttttaca catcggtaac agagattgaa cgtgggtgtg atgcctcgca attacaatcc
    54301 caccgctttc acgacattaa cagacactac aaggaggatg ttcgtaatgg aaatgcgtcc
    54361 gttggttgca tccatcagcg gccttgtcgg gagcgcgatg ctcgggctct gcgtaacagc
    54421 gtcctgggcc gatgattcct cacgccaggg ccagtcgtca cacaccgtgg tagcccaggc
    54481 aatgggcccg gctccgaacg gaaccagcag tccgccgcct gcgccggata tgcctctggg
    54541 cggcacgtca atcgaagacc tgcttatgca gaagggcacg atcaccaaag aagagtggat
    54601 tcagattcgg gccgaacagg aatacaaagc tggagagcgg gacaaacggg tcgatagctt
    54661 ggacgagtgg aagtccaagg ctgagttgct gccgatcctt agggataagg tgaacttcgg
    54721 actcaacgca ctccaatggt tgtacacgca ccaggatgcg catgtgcccg aaggacggag
    54781 tcaggacaac ctatccatcc gccggtcgga aattctcttc tggggccgta tcagcgacac
    54841 cctgccgcga tggcacacct tgatggagtt tcaaagcatc aatctcttga acaacacacc
    54901 gacaggaggc agcgcggcca ctggcggagt gccgacctcg tcgacattct ttcgggaagc
    54961 ctacctcgac atacgaccgg tgcagtcgtg ggcgcccaac gtgaattatt tccgcatggg
    55021 tatctttcga atgcccttcg gcatcttcac ggaaacctcc ggcggcttgc gcgatgtcat
    55081 cagctctccc taccttacct cggtgggctc tggggtcggc aatcgtaccg ggtcggctgg
    55141 caccgtcgag tttctccaag agcgggatta cttcctcgat atgcgcggga aactgttcaa
    55201 ccggctggag tatgtggtcg gtattatgaa caacaacaac tatgaggcca acgcgaccgg
    55261 atccaacgcg ccgaaaagct tctactcccg cgtccgattg ttcgggagcg acgtgtcatt
    55321 tatcagtttc accacgattc aaggcgagtc gaacaacgcc aacaccaaca tcaatggtcg
    55381 cggcaaggga gccttcgatc gctatggact ggactttcgg tatacctcga aaattcttcc
    55441 tggcttcatg gtgcagggag aaatatggca ggggcatgat ggggccaatc agacgacagt
    55501 cggacgcccg gccaacggtg cttgcgatgt ccaagccatc tgcggcggct ccggcgcacc
    55561 gggggtacaa cgacgtacct ggtacgtact ggccaaatat ctcatcactg acggcccctt
    55621 cgaaaacttc gagccggtca tcatgtatga acaattcgac ccgaatacca ggatcggcaa
    55681 tgatctctac accagaacca tcgtcggctt gagctactat ttcgaaaact ttccgccgaa
    55741 ggtgcagtcc aaactgcaag tcaattacga gttccgccat cactcgggca acgggcctaa
    55801 caatgtcgtc gatccgacgc gcggtgtggg cgaccctttc gcccagaatg ccttcttggt
    55861 acagtggcaa atccgattca tgtaacggga tgactgcaac tgcggcaaca actgtctaac
    55921 cgattcggtc tcgagcaaag agaaaggcta gcaatgaaga tataccgggg agcgatcgcg
    55981 gcggtgctga tcggtttagt gctcaacgga tgcggaccac aacgcgtctt ctcacctcaa
    56041 gtgctggacg gggtggaccc aaatttcgac tttacggcct ggcgcatggt gccgaatgcc
    56101 aaggcgggga aaaaggtgca actgggtggt cgcatcctgc aggctccgca aaaagacggc
    56161 ctggtcacca tggtgctgac ccaattgccg attgtcgaac acccggccta tggtccgacg
    56221 gatacggaac gggggagggg tgaatttctg gtgtatttta acggcaaggt tcccacaaac
    56281 catctgcagc ccgggaatcg cgtcattgcc atcgggacca cgcaaaacgc ggcggtggtc
    56341 gctatcgagg atagccaacg cagtctgccc gctctcacgg cccagtgcct acatatctgg
    56401 aacaccgggg gaaaagagat tgctgatttc ccgaactttg gggcaggata tcagccactc
    56461 gaggaggaga cgtattgcgc cgaatcccgc tgacaagccc tgaaagtatt ccaccttcac
    56521 gagggcggct tcgcacactt gatgtgaccg ccgctctcgt gctggtgtgg gcatgcagcg
    56581 cctgcggagc cggcgtgacc ctagttcgcg acacgccgac aggagggctc gtcacgtacc
    56641 ccttccaaaa tgaagtcgat gtcctcactt ccagcgagcg gcgtgacgcg atggagttga
    56701 tcaaccgcaa atgcccggct gggttcaaag ttcagaaaga aggcgaaata ccaaaggtga
    56761 gccgcgctgc ggatcgagcc tggcgaggac aaatgggagg ggacagagta tggggaattc
    56821 agttcacatg ccagtagcgt cgatggtcac cataccctat ccccggctgg cattgcgccg
    56881 cagcctctgc ctcgttctcc tcatgtacct gacctccgca tgctcgctat tgaccggagg
    56941 aaaggtgctg ttcgaccaac aggacagtca ggtgggagtg caaacggacc cgactgtcca
    57001 tcgcggacct gcccccacgg aaaacaacca tcctgcgcag ctgaccacgg aggaggtgcg
    57061 cgtgcttctc ggttcgttga tggtgagtgg ttggagcgga acgctggtgg gcatcctgga
    57121 acggccccgt ccgatccctg tcttcactga gctggaattg aatcggatcg ccgcgccggt
    57181 cgcagccgct tttcaacaag caactcccag cgagcgcgtg tttttttcct tacctaaatc
    57241 acagacgacc tatagcgaag aacggaccac cggtgcgctc tttcttcgag gacgatatct
    57301 tcacgtcgtg ctcacggacc actccgcctt cacgagggcg gacacggccg gaggggaggt
    57361 gaaggatctc cgagatacaa aaggaatgaa gttgtgggtc accgctccgg caacagcagc
    57421 caccgtaccc gatgcggaag agccacgctg ggctcatttt gaaacagtac acgtctccct
    57481 gaatgccaag gaggtcattg ctgccagaaa ccggcctgct tctccctcgg ctgacgtttc
    57541 accctcaata tcgcttcacc catcagaggc agtgggagcc ggcgcaaaga gcaacgggcc
    57601 agccgacacg accggcaatc agaccgatga gcttcgcctg caagtgcgtg agctgacgac
    57661 ctcaaatctg gaacttcgga atcgtttaga tactcaactg aaacagatgc aggcgttgca
    57721 ggatcaatta acaaaacttc aacaagacct tgactccgct cgagcaaaaa gcaagacacc
    57781 tcgccgatct acaaccccat agccatccac acctttacta tcagccatct acgcataatc
    57841 cgttgttgga atcctcgctc atatcgtgag acacctcggt gtttctcctc gagaggagta
    57901 ccggcccagt gagcagcgat tgcgcgatcc aataaggcga gccgctcacc tcttcgcatc
    57961 cgtgatccgc tgaatcacgc caacctgacg gctgcgcatg ttcggcgcct cacaatctac
    58021 acattggcgt aacatccggt atcgtcatgt gctgcgttac ccgctcggcc gctcgcatca
    58081 ggccgaagtt ccggccccct aggtcagcca ttctgtcggg cgaggacggt ggtgcaacgc
    58141 acgagcaata agtattcagg ctatcgataa ttctcgaagg cgggtagcct ctctcgaatg
    58201 ctggcgggag acgcagttgg tcagcgagga aaggatgaga acggtttgca ccgcaggcat
    58261 cgaggaatag tctcgatgac ctgcggcagt agccattatg cgaaacgacg gtggatcagt
    58321 ttgggagggg agtctcttgg ccggaaaggc cgagcgccag aaagttcttt ttcagctggt
    58381 tcatcggaag agggaaaaat cccgccttca tcacggcttc ctggccttca cgactcgtca
    58441 caaacgtcag gaactcttgc acagcagggg cgagggcagc cttgggagat ttatcgacat
    58501 acagatagag catccggcga aggggatacg tttggtcgga aacagttgtc gccgtcggtg
    58561 cgataaatgg catgccctga ttctcagcga gcggaacgat ccgcacattg gacgtttcaa
    58621 ggcccaatcc actgtatccg atacccaatt gatcgcgcgt cagatcaaga atgaccgatg
    58681 cggcacccgg ttcctcccga acagacggtg cgaattcgcc accggctaag caatgttcct
    58741 gaaagaacgc gcgcgtcccg gatcgccgat ctcgaccata gagtctgacg ggagccttct
    58801 cccaaccgtc ttgcagtccc agagcccccc attgcgacag gctggttttt aatccgcggt
    58861 tgtgcgtggt tgaaaacatg gcatcgacct ggtcgagcgt gaggccttgg atcggattat
    58921 cacgatggac atacagtgcc acggcatcga ctgcgacggg gatggcggtg ggttcatatc
    58981 catgctgggc gacaaattgt ttcacttcgg catcaagcaa ctcgcgtgag gacgccacca
    59041 gggagacata ggtcggtcgt tcttccttga gggtaatttt cccgctcaat ttggcgggcg
    59101 gctggacgaa ctcgttcaca gctttggtcg atccaccccc gctcacatta atcgagacat
    59161 tcggctggcg tcgttgaaac tcacctgcaa gacgactcat aagcagagac atcgtatcgg
    59221 aaccttgaac ccgaaggctt cccgccactt gagtctgcgg attgtattgt accaatcctt
    59281 cttgaatggc cagactcgcc gttggtccgg cgttctcggc ccgtgcgact cccgccggaa
    59341 gcacagccaa cgactcagca gtagcaagga ttcctgccaa tcccacaacc actgctcgcc
    59401 gtcgccatcc atgcttctgt ccatgactta acatgctctt ctcctccgct agagtggtgg
    59461 tatcggctat aactgtatgt atcctactaa ccgatgattg ctgcttcatc actggcatgt
    59521 tacggatgtg ttaaatcgtc ccctagagtc cgatgccagc gggatggacc ccgttggagc
    59581 gtgaatgcag cctgacctga tcgtgctcgg tcttaaccac cgactgaacg gatcctcttc
    59641 gacaagcctc gactctctac gcaagtagca cttcgatagc acgaagacaa ggccactccc
    59701 ggcagaaact gctacaccgt tgccctcgca tgtcgagacg gtattcgcag ccataccagc
    59761 caagggatgt ctccgatgtc gtgaggaaca ctgtcggctg agggctgtga ctgacacaag
    59821 gaagagtgaa cgggagagag gtttcggagc aacgtcttgc ggctgtcgga gctccgacca
    59881 cacacgcgat cggagcgctc agccaaacga cgaacgccaa tgcactgtga aaaaacgatt
    59941 agaactcgtc caccgcgacc ggctgcaaga ggtcgcgcac cgagagcacc ccgacgatcg
    60001 cgccgctctg caacacgccg agatgacgag tgtgatggca ctgcatgaga tcggcagcct
    60061 cggtaatcga tcgccgctcg tcgagactga tgaccgggct gctcatgatc gattcgaccg
    60121 gaacaaaatg caccggtttg ttttctccga caacttttcg aacaatgtcg gactcggtca
    60181 cgatgcccac aacacgattg ttctgctcga tgaacacact accgacatga cgctcattca
    60241 tcactttagc ggcctcggcc gtcgttgtgc caaccggtac cgttaccagt ttcttcgtca
    60301 tgagctcgct gacagtcacc atatccgatc ctcctggtta gatgggttga ctcagaaaca
    60361 cgcccttctg agtctctaac tcgaaggata gaacgggcat attgaaagcg ggttatgggc
    60421 aggtaaaatc tttgttacga ctgacggaga gtcggaagaa cgtcgaacca agcgcggcat
    60481 gggccacgta cgcccatgcc gccgtcgtca caaccagatg gggggagttt attctttcac
    60541 gacgaagtgc acactgcgat tcttctgcca acagaccgga ttatgctgca aacacagggg
    60601 acggtctttg ccataactcg tgacatccac ctgcacctga ctcatgccga gtgattccag
    60661 gtaacgcttc acggaaaggg cacgacgttc gccgagcacc aagttgtatt ccgcggtgcc
    60721 gatttcgtca caccggcctt ccaacaacac tttggtgacc cgattgtctt ttaatcgttt
    60781 ggcattcgcc tcgacggcat tcagcgcatc gtcccgcaga acatactgat cgaaatcgaa
    60841 caacacatca gggaagggga tattctgttc ccccgtcgca ttacgggcgg ccatttccac
    60901 tgagcgcaag ggaaccggcg ccggttcggg agccggagga atttcctgaa caggagcagc
    60961 agcaatccgc tcctccacac ggggtttgga aggagttacg gtggctcctc cactggttcc
    61021 cgccttgctc gcgcagccgt caaacagaaa gggagcggcg acggctaaca gtccaaccaa
    61081 ggcaaacggg cgcaaaacga tcatgaaaac ctccggcgct aggtgagttc gtggaccaga
    61141 cgaggcacag aataatcaaa agatgcgaac ctggcaagtt acttcttccc ggcacaacga
    61201 tcgtagaccg atcgccactc cggcaagggg ggccactgca gaatcgtgag cgcatcctcc
    61261 acataggccg gcgtgcgcat accgttgagc acacaggtga cacccggcgt gctggcgagc
    61321 acccagaggg ccttttgcga caaggtggcc gtgcgtcgct gttccggtag aaggggattc
    61381 atggtgtgat ggagttcatc ggagcggagg cggctttttt ctgatgcctc ccgatgcaga
    61441 attctcagga gcgccaacaa ttccggcacg tatcggtccc gccaggactc ccactgttca
    61501 gcgacgtttc cggtaaacgc ctcggccagc gcacgaagca cctggttcac gtgcggcgcg
    61561 atcatctgct gttcgatttg ttcccaatgc tcgagcccct gcacctggcc tcgtattcga
    61621 gtcaactcat cggcccatcg aaaaaagtcg gcgggtagca tgccctgacc gctgtgcgcg
    61681 actgcagggg cgagactgtt gcgatattcc tcctccaatg cggcgacttt ctttcgctgc
    61741 gcctcgaaat cggcctctgg agccggcacc gagacgtccg ccaatcgaat cactcctccg
    61801 cgctgcacgg gcatcgcatt gagtggacga ttgaccagca cggcgatccg ctcgcgcatg
    61861 gcttcctcca gcagcgtgct gccgttgtga atcccggtgt ttttgatcag agctgcgccc
    61921 gactcgtaca cattcatcgg acattggagc acctggaagt gatgggacgg ggctccaacg
    61981 gtcgtggccg ccaacttcgc cgcgtcgatc atgtgagaca gggaagtggc accgggatcg
    62041 tcgggagctg acgtggccgt attcgatgag acaccatagc cacgcaagcg acccgcctga
    62101 acctgcgtct cgaaatactc gaaggcccgc tgaagtcgcg cataaaactc ctggcgtaac
    62161 gcaggcaggt cgcgcgactc atcgccgccg agacgcgtgg catgggacag gaaatattcc
    62221 ggattatgga gcaggcacac atcgagcgtg gctaacccca gccggtccaa cgacagcgtg
    62281 agttggtccg ccagaaactc cgggtggatg caatgccaga tatcgtcacc atacttgacc
    62341 atgtcggggt agggtttgcc cgatttttcc cgcgcctggg cctgcgtcaa attctggccc
    62401 tgtacgtacc cgatcttcga caccacgatg acgccctcgc gggttagatc gcccgcgcgc
    62461 atcatctccc gcaggaccga tcccaccaat tgctcactct caccatccat gtaattggtc
    62521 gaggtgtcga tcacattaca gccctggcgc aatgcgctgc gtaaggcctc acgatgttcg
    62581 gcctcccgct ggccgactcg atacgttccg aatcctaatc gactggctgt cagtccagtc
    62641 gcaccaaaca gggcgaatcc atgcgcgagc gccccgttgc tctcgtgaat gtgtcgcgca
    62701 gcataagccg ctgtgccctg cacggtcgca tggcccgggt ataacgttcc gctcaagatc
    62761 ttctgctccg atgcgggagg tgaggaggcc tgttgccggg gatcaagcag agagggaaat
    62821 tctttcggat cggtgatcgg ccggccgcaa gcaaaattcc ggcacacata cacggctgcc
    62881 ttgccgtcga tcaacgtctt tccctgcagg agcggatgac tggttgcgct ctgatctgag
    62941 tcgcgatgaa cgagcacgcg attgggaagg taggtgcgat tcacggcggc ccgtaaagac
    63001 tccatccctt gatccccgag ccgcccgacc aacgcaatct ccgcaggccc actcatgagg
    63061 aaatcgacca cgatgaggct cttggcaaat gcccgcgggt agcgcgcaat ctgacggcca
    63121 taggcgcgaa cggcggcggt cgcggcctcg cggaaatcct cccgggcaaa atgagaagac
    63181 aatcgagcca gcaccgaggc agccaccgcg ttgccgctgg gcgtggcccc gtctggtccc
    63241 tcacggctgc gcatgatcaa cgcctcatgg ccggtggcgg tggtgaagaa cccaccgtgc
    63301 tggctgtcgg caaaatccgc cagcatccgc tcggcgaggc gaaccgacgc gagcagatag
    63361 cgttcctcgc ccccggcttc ataggtatcg atcaagcctt cagcaaaata ggcgtaatct
    63421 tccagatagg cattcaggtg ggcggtaccc gcacgatacg ttcgaaggag ccggccatcg
    63481 gcagtcgaga gtgtcgtcag cagaaaatcg caggcccgtt cggccgcttc gcgataacgg
    63541 ggtatgtcaa acacccgtcc ggcttccgcc atggcgctga tcatcatccc gttccacgcc
    63601 gtgatcacct tatcatcgag gccgggcgca atgcgcttcg cacgcactgc gtaaagcttc
    63661 ggtctgagac gggtgatggt ttcagttaac tcgccggcag tgatgctcag ttccttggcg
    63721 accgcctcga cgctcttcgc ggtatggagc acattcgtgt gttcccagtt accccccgcc
    63781 gtcacatcgt aataggcgca gaagcggcgc gcctcttcat cactgtccac cgccgctcgc
    63841 acgtcttccg gcgtccacac aaagaacttg ccctcgacgc cttcggaatc ggcatcggtg
    63901 gcggagtaaa accctccctc gggtgacgtc atttccttga ggatataatc cagcgtctcg
    63961 caggcaaccc ggcggtaggt ctcgtctgcc gtgacctggt aggcttcgac atagacgcga
    64021 gccagcaacg cattgtcata aagcatcttt tcgaagtgcg gaaccagcca acgttcgtcg
    64081 gtcgaatacc gagcgaaacc gccgccgatg tgatcgtaga ttccgcccgc cgccatggca
    64141 tccaacgtct tgcgcaccat cgtcagcgta tggggatcct tcgtgcgctg gtaacaatgg
    64201 aggagcaggg acaaccctgt ggccggagga aattttgggg cgccgccgaa gccgccctgt
    64261 ctcgcatcaa aatcttcagc aaactgggtc acggccatat cgagttccgc ttcgccgact
    64321 gtcgtcggag aaggcgcgtg agccccctct tggagccgtg ccgtcagatt caccgcctgc
    64381 gcaaccacgc catcgtggtc cttctcccag tactcggcga tcttcttcaa cagggtcggg
    64441 aagccgggac gaccgtagcg atctgtcgga ggaaaatacg tgcccgcaaa aaacggtttc
    64501 tggtccggag tgagaaacac cgtcatcggc cagccacctt gattgcggtt caacgccaag
    64561 gtcgcctgca tgtagatttc atccagatcc ggccgctctt cgcgatcgac cttcacgcag
    64621 atgaagtgtt ggttcatcag ggcggcaatc gcctcgtttt caaacgactc ccgctccatc
    64681 acgtgacacc agtgacaaga ggaatacccg atggagagca ggatggggcg cttcagtgct
    64741 gcggcctgag ccaaggcttc cggcccccag ggataccaat ccacggggtt gtacgcatgc
    64801 tgtaagagat agggactggt ttctcgaatg agccgattgg cggggcgatt cgcgtggtga
    64861 tcagtcattc gcgaaccgta acacgggctc agtgaatggg tcaatgaagc ggggagccgg
    64921 gcagggaagg ggcggaagag ataaaaaaat tggcccgcgc cggcaatccc gggtcgcgag
    64981 ccaactttat actacgacag aacgtcgaaa gcggggctca gtggccgccc ttcttctcgt
    65041 ccttcttctt ctcttcgccg aacacggtct gggacaggtg tccacccttc ttctcttcct
    65101 tcttcttgtc gtcaccgaag agggtctggg agaagtggcc gcccttcttc tcttccttct
    65161 tcttgtcatc tccggcgaaa gacggagcgg tgaacgcaac gagaatggct gccgctacga
    65221 atgcgaatag agcacgcttc atggaaaccc ctccttgaaa ggttgagaat ataagtgccc
    65281 acaccacaag ggcgcccgag ggtagccaac ctgtcagaaa aagtcaacga aactctttgc
    65341 gtatatttca ccagcctcgt catccaacgg atgcgtcacg gcctcctcct gccggggatg
    65401 tctggagcgc tttacttttc gagacgttaa cggatctcga cagcctatgg ttcaccgcga
    65461 acccatcaac gctcggcctt cacgaagtgc caccctgatg ggacacttct tcgtgagata
    65521 gacgaagtgg aaggaggcca atgcaggaaa cgtcatgctt caccagaagc tcagatccgt
    65581 ccgcgctgtg atacactctt cataagtaat ccacacggtc tcggatcaat cctatgacca
    65641 ccgacgacat cggcccgtac tccaactacc gcctgaccgt ccgcctcgcg ctcctcaaca
    65701 aaccgggcat cttcgcgaaa gtggccgccc tgcttgcgga ggaaggagcc aacctggggg
    65761 cggtcgatat cgtgtccgcc aatgcggagc gcatgatcag ggacattacc ttcgatgtgc
    65821 agaatgaggc gcatggcgac cgggtgcttg agcgcctgga agcgctgccg gaagtgaatg
    65881 tcctgtcggc ctcagaccgg atcttcctcc tgcacctggg cggaaaaatc agagtccaga
    65941 gtaaagtccc ggtcacgacc cgcaacgttt tgtccatgat ctatacgccc ggcgttggac
    66001 gcgtctgcaa agccatcgcc aaggatccgg taaaagccta tgccttcacg agcaagagca
    66061 atagcgtggc cgtcgtcacc gacgggtccg ccgttctcgg gctcgggaac ctcggcccgg
    66121 cagcggcgct tccggtgatg gaagggaagg tgatgctctt tcgggaattg gccggcatcg
    66181 acgcctggcc gatttgcgtc agcacacagg atccggaaga aatcatccgc atcgtccgcg
    66241 gtatcgctcc cgggttcggc ggaatcaatc tggaagatat cagcgccccg cgttgtttcg
    66301 agatcgaacg cgctctcaag acctcgctcg acattcccgt catgcacgac gaccagcacg
    66361 gcacagccgt tgtcttgctg gcagcgttga ccaatgcgct gaaggttgtc ggcaagcgga
    66421 tggaggacgt tcgcatcgtg gtgaatggcc tcggcgcagc aggaacggcc tgttgccgca
    66481 ttctgttggc cgccggagcc tgtcaccttg tcggatgcga caaggaaggc atcgtgttga
    66541 tgggggaggc agaggaactc cgcgcctgcc ggaccgacct tcaggcctgc atccggcggg
    66601 accagccgcg cggcaccatt cacgacgcct tgcgcggcgc cgatgtcttc atcgggctct
    66661 cggtggggaa tgtgctggag aaagccgatc tcgacaacat gaactcagat cgcatcgtgt
    66721 tcgccatggc caatcccgat cccgaaatcg atccgaagat cggcgatgaa gtgtcgcgca
    66781 tttttgctac cggccgatcc gactatccca atcaaatcaa taatgccctc tcgtttccgg
    66841 gaatttttcg cggtgccctc gacgtgcaag ccagcgagat caacgaagcc atgaaattag
    66901 ccgcggcaca ggccattgcc gaatgtgtgc cgaccgaggc cctgtcggaa gactatatta
    66961 tccccagcgt cttcgatcgc gacgtcgtgc cgcgggtggc caaggccgtg gcggccgcgg
    67021 ctcgcaccac cggtgtcgcc cgacgccgca tcaaaatcga agacgacccc cgctgaccca
    67081 ggccgctcac cgttccgatc tccgcttgcg cacagtgatc aatcgttttt ctgcaacatt
    67141 ggcgtggtac gcagaatcca tgtagagtag ggccgacgct cgaggccgtc gcccgtctct
    67201 cgtggctttg cctccgcctc agtgagatca tccggaatcc agtgtgacgc caaccaccat
    67261 tcaactgatc ggaaccctgt tgttcgccgt cgcgatcctg cacacgtttg cgacggcatt
    67321 cttcgaacat ctggcgcata ccagaccggc acatgccggc ctctggcatt tgctcggtga
    67381 agtcgaggtt gcgttcggat tctgggctct cgtgctcatc gtcgccatgt ttgtcaccga
    67441 cggtgcggct gccgccacgc actatctcga ctcccgcagt tttacggaac ccctgttcgt
    67501 cttcgccatc atggtgatcg caggaaccag gccgatctta cagaccgcca tgacgggagt
    67561 ccgtattgtg agcagtgcca tcccgctgcc acagggaatg cgggtgtatc tcaccgtgct
    67621 ggcacttgtt ccgctattgg gatccttcat cacagaaccg gccgcgatga cgttggcggc
    67681 actcctgctg cgtgatgtct tttttcgcca tggctgctcc acgaacctga aatacgcgac
    67741 tctcggcgtc ctcttcgtga atgtgtccat cgggggaacg ctcactccct tcgctgcgcc
    67801 gcctgtgtta atggtggccg gtgcctggca gtgggatatc gcattcatgc tgggcacatt
    67861 cggttggaaa gcggcgttgg ctgtattgat caatgcgttt ggggcaacca tgctgtttcg
    67921 tcgagaactc agccgcatct cgaccgccat cgatcaacgc gaggagaacc gcgcgccact
    67981 ggctctggtg atcacacatc ttgctttgct cgccatggtc gtggtgttcg cccaccatcc
    68041 cgcgatgttc ctgggcatcc tgctgttgtt tctcgggatc gcgcaggcct acgagcagta
    68101 tcaggatcgt ctgattcttc gcgaagggtt gctcgtctct tttttcctgg caggcctggt
    68161 ggtcttaggc ggacaacaac aatggtggct gcagccgatc ctgctcggca tgggagccga
    68221 tgcggtgttc gtaggcgcca cgattctgac tgccttcact gacaatgccg ctctgaccta
    68281 tctcgggtcg ctcgtcgaag gcctttcgga tgcatttaag tatgccctgg tggcaggcgc
    68341 cgtcacgggc ggtggcttga ccattatcgc caatgcgccg aatccggccg gactggccat
    68401 cctgcggacc tatttcgacg acgaggcggt acatccgctc cgactgttcc tggccgcgct
    68461 gccgcccaca ctgatcgcgg cagccatgtt ctggtgtctg tagcctgcga cgcagcaacg
    68521 attcactcaa tcgactctta tcgccggaca aagtgatgtg ctaagatgcg atcgacttgc
    68581 ctcttacctg caaggtcacg cctaacgagg actcgtggca ttttcaacca atcgcctgtt
    68641 caaatatcag cggggcatgc ggcgccggat cggcatgctg cgcgccaaga atatcgacag
    68701 catggagttt gccgtcgatt tcatgatgca ggaacgggtc gatagctatt tccgcatcga
    68761 accggccttg gaagaagtcg aacgttcgct gggacagatc gaagacgaat tggacctggt
    68821 gaaggatgtg tccggggcca ttcgtttgga atcacgcctg gaattcgtcg aagatcgctg
    68881 ggatgagctt gacagcgaga tacgtgaacg cccgcgacgg cgccggcgaa aaatcaatct
    68941 cgccgatttc ctcaaagccg ccggagggga ggggaatccc gccggtacgt ccagcgaagt
    69001 cacgaacgcc tacgacgcct atcagttgct gggcctggaa ttcggcacgc cgctcgcaga
    69061 cgtcaccaca gcgtttcgtc aacgcgccaa ggaattgcat cccgatgccc ggaacggcga
    69121 ccgcagttcc gagcccgaac tccggcgcat tctcgaagcc tatcagttcc tcaaagaata
    69181 tctgagcttg agcaacaccg aaccgccgat ttcacgcggc gcggaatatc gtcccaccga
    69241 gtaacccatg gccacattgg atccggatca gaccttcatc acctcagacc atatgctcga
    69301 ccggttatgc gaccagctgg ccaacagcgc ggtgatcgcc atcgacaccg agttcatggg
    69361 tgaagaccat ttcattccgc gcctggaatt gatccaggtt gcggcggaag gggtggctgc
    69421 cgtcatcgac tttcctgccg tgcaggacag cgcgccgatg gtccgcttct gggaactggt
    69481 ctgtgatgcg cgcatcgaga aggtgcttca tgcaggtcgg caggatctcg aactgttcgc
    69541 gcaccatgcc ggccgattgc cgaagccgtt tttcgacacg caaattgccg ccgcgatggt
    69601 cgggtacggt gcgcagaccg cttacgccaa tctggtgcag cgggtgcagg gggtgaaact
    69661 cgataaagct cacaccttca ccaactggag tcaacgaccg ttgagcaagg accaattggt
    69721 ctacgccctc gatgacgtga ccttcttgct gcctatccat cggcatctcc gtcagaaact
    69781 gtcggtgatg ggccgtttgg actgggtgga cgaagaattc tcccgcctgg aggtctcgct
    69841 cggagcccag gctcgcgacc cgcaggagcg gtaccagcgt attcgcgggt gggacagcct
    69901 caagccgcgc gccgccggcg tgttgcgcga cctggccgcc tggcgggaag gggaagcccg
    69961 ccgccgcaac gtcccacgag ggcgggtgat gcgcgatgaa gtgttggtgc agttggcccg
    70021 tcaaaccccg cgcacgctcg atcaacttcg cggtatgcgc ggcatgtatt cctcggaagt
    70081 cgaacgcaac gggcaggccc tgcttgccac gatgcagcag gccttggcca gaccggaatc
    70141 cgagtggccg gaagtgccac gcgaccggaa accggatcct gaatccaccg gggtgctgga
    70201 actgctgcag gccgtgctga aatcccgcgc tgccatggag aacatcgccc cgactctcat
    70261 cgccacgacg ggcgatttgc aggccttggt ggaacggacg tcgccggtgg aagaagttga
    70321 cattccggtc ctgcgaggat ggcggcgcca actggtgggg gaaacgctgc tcagcgtctt
    70381 atccggcaat ctcaaagtct ggatcgaccc gcaaatcggc aaactccgct tcggacattt
    70441 ggaccatccg tagtcgagct cacctcgacc gaaccttctc tgcccctcac cggaatgcct
    70501 tgccagccgt tcgctgtcgc ggctgatgcc gacgatcctt ccacatcccc aacaacagcg
    70561 tcagcaatgc gaacccgatt tcgtcggcca acggaatcac atccgggaca atcacatcca
    70621 ccagggtgag aacggcgaac aacacaaaga gggatgggaa gcgaagattg agccgctgca
    70681 gaacccgtcc cagcagtgac acccgatcta cggaatcgaa agccaacatc tctgtccctc
    70741 cgccaatccg ctacccacgc cccgccttgc atcgtctctc tcgggaacct tagagcaatt
    70801 cggtgacggt gtcagccggg cgcgccagca tgaccttgtc tcctttgacc acgatgggcc
    70861 gctgaatcaa atccggatgt gcgatcatct gatccagtaa gtgatcgtcg ctcaacgtcg
    70921 aatccgcaag cttcaacgac ttgtagagat cttcttttgt gcgcaagaca tcgcgcgggg
    70981 acaggccggc tttcttcagc agctctttga gccggctctt cgtaaacggt tgttcgtagt
    71041 agttgaccgc cgtaaatggc tgcccgctct cccgcaccag acgcaccgcc tctcgacagg
    71101 ttgaacaggt gggtttctga tagatcgtga tctccgccat gccgttaacc tttctccttc
    71161 atgagggttc ccttgacgca aatttttcac ctgtgttaca tggacacaca agattcatat
    71221 caacgcgatt gtgattaccg atgcccatct ttggaaccga tacgagcgac cagaccggca
    71281 aattctcctg tgccaccgcg acacagcgca ccttgaaaga tttgcgcacc aaacgcaagg
    71341 gccaaccggt gtttgtcgtg ggacatcggc tggagcgtaa ggggaaagaa gccacgtttg
    71401 aggtcttcaa tgaccgcttg gccatcatcc ggttcgaaga tgacgtccgg ctgggctacg
    71461 atccgttcga actgctgctt cccacggaga tcgatgacaa gggcgtggcc tatttcgaga
    71521 tccgcacctg ccggctctgc gagcagtttt tccccctcac ggcggatgaa tgtgacgagg
    71581 aagaagaacg gacaacctgc ccggagtgcg cctgacaagc aggatacgac tcaggacgca
    71641 caactacata ttcccgactt acgatagact tgtcactctg ccagcggcgc aatctcgacc
    71701 gctttcttga tcaggcaatc tcccgcgaac ccttgcagtc ccatcggcaa catgctggca
    71761 tccagcaccc gggctgacat caccttcaat ccgtcgccga ttttcatacc ttcgatcttg
    71821 agtgcgtgac agacttccag cgtgcgccat acgggattgg cgacgaccga ctcgctcgga
    71881 atcagtttga tgtcggagga agcgccgtcc tgaaggacct tcgcagaaat acgcgtctta
    71941 tctttcggca catcactgac gaccgcgaaa aagacctgct catcatcagc ccaggccggg
    72001 acattcagac caatgaccat tagcactccc agcgcgagcg accgaaaccg gcccatgcct
    72061 tacctcgttg ttgcgggtgg agcctgtgtg gacgattcat tcctctcgcc tgcaggaaga
    72121 cgtggggaat tgtcggactg agatccggcg ccgggaccgc catgactcag ggtgctgcgt
    72181 cctgcccgtg gggatcccgg ggcgacggcg gaacgttctt cagtcggcgg gtctccgatc
    72241 cgcaacatcc acagagcgac gcctgcaacc aacagtccga ccagcagaag aatgcgtacg
    72301 aatggagtca ccgtgttctc cgcggtcgaa gtcctcttcg tttcgaggta gtatgccaag
    72361 cgaatccatt caattcaagg tgaaggctga gtcaatgccg tcgatcgtga taagctgagg
    72421 ccagacgacc gtcactcagg aagcccggac acgagggccg ctcatggaac cgatcgcatc
    72481 acgcgtgatg ccccattcgg attgttgaag ataggagaca gaatctatgc cgcacgattt
    72541 catgccgggt ctggccggtg ttcctgccgc aaaatcagcg atcagcgatg tggatggaat
    72601 acgaggcgtc cttgaatatc gcggaatccg ggtcgaggag ctgtgtcagc actcgtcctt
    72661 tctcgaaacg agttacctcc tgttgttcgg acggttaccg actgcgacgg aactcacgca
    72721 gtgggtggat gatgtcacac accaccggcg gatcaagttt cgtattgtag acctgctgaa
    72781 ggtcatgcct gaacatgggc atcccatgga cgcgttgcag gccgcggtcg cggcgctggg
    72841 catgttttac ccgggccgca acgtcaagga cgttgaaaac aactactggt cggcggtgcg
    72901 actcatcgcg aagctgccta ctatcgtagc cgcctgggcg cggttgcgac ggggagacga
    72961 atatattccg ccgcgcgacg atctgccctt tgccgacaac tttctctaca tgctgacgga
    73021 aaccgttccg cacccgttgt ggagcgcggt tttcgatgac tgcctgatcc ttcatgccga
    73081 acacaccatg aatgcctcca ccttcgccgg catggtcacc gcgtcgacac tcgcggaccc
    73141 ctataccgtc gtggcctcgt cgatcggcgc gctcaagggc ccgttacacg ggggcgccaa
    73201 tgaagaagtg gtgacgatgt tgaaggagat tggcacgccg gcgcaggctc gcgcggcggt
    73261 ggaagcccgg ctcggcggga aaaacaaact catgggattc ggccatcgcg tgtacaaggt
    73321 gaaagatccg cgcgccaccg tgctacagga tctttgcatg cggctgttca atgtctgcgg
    73381 gacttcgccg ctctacgaag tcgcagtagc cgtggaacag ctggccagcg aacgattaca
    73441 aggaaagggc atttacccga acgtcgattt ctattcgggc atcatttacg acaagatggg
    73501 aatcgaggtc gatctgttta cgccgctctt cgccatggcg cgggtgagcg gttggctggc
    73561 gcattggctg gaacaactgc gggaaaacaa actctaccgg cccgatcaga tctattccgg
    73621 ggaacatgac cggcattacg tgccgatcga tcggcgataa accgacggcg aactattcgg
    73681 tacgaccgat gaaccaccat acgaccatgg acgcaaccat caagataccg ccgagcaatg
    73741 cgtaggaaat gaattccaac atggtgatcg tctccaatat gaagggggct aataaataat
    73801 gccgagggct gcaatgtcaa gagcccgctc atgacaatta ctcttcaggg cacacccttg
    73861 acttcaccgg cgtggagatt accttccttg tattaagtag gatggctcaa gtgaacacca
    73921 gcaacgcatt cacacaagga ggttccacca tggcgatcgt acgttgggat ccgttccggg
    73981 aattggaaga aatgtctgac cggttgaacc ggatgattac acggccgggc gcagctcaaa
    74041 ccgctactca gggcaaggaa gtgatgaccg tcgctgattg ggcgccgacg gtcgacatca
    74101 gcgagaccga agccgaatat gccattaagg ccgagcttcc agaggtcaaa aaggaagatg
    74161 tgaaggtcac ggtcgaagac ggtgtcctca cgttgcaggg cgagcggaag caggaaaagg
    74221 aagacaaggg gaaaaagtac catcggatcg aacggtcata tggacgcttt gtccgaagct
    74281 tcacgcttcc cgacaccgtg gatgagagta aggtgaaggc ggaatatacc gatggtgtgc
    74341 tccacctgca tctgccaaaa tcagaaaagg cgaaaccgaa acaaatcgac gtcaaaatcg
    74401 cctaagacaa cgaaccccgg tgagagagca aggcccgcct tccggcgggc cttttctttt
    74461 tcagttcgtc cctgaacttc ttcaccccat acaacaactc cctgagcgcc ccctcgttcc
    74521 tctctccaag gccatgcttg tgcttcagaa gggcgagcgt ttattatgcg cggttcaagc
    74581 agctcaaccg ccagtcattc gaggagattc aatgatgaag aacgcttcgc gcacgcccaa
    74641 gagcactgca tcagcgacaa ccgccacgcg catggaacga gacaccatgg gagaacttcc
    74701 ggttccctcc aatgcgtatt atggcgtgca aacggcccgc gcgattgaaa actttccgat
    74761 cagttcgcta cgcattcccc gcgccatgat tcgggcgatg ggactcatta agcgcgccgc
    74821 cgcctccgtc aatcactcgc tgaagttgct cgataaaaaa ccggcggacg ccatcattcg
    74881 ggccgcgacc gaagtcgttg acggggggct ggacgccgaa tttccggttg atatttttca
    74941 gaccggctcg ggcacgtcga cgaacatgaa tacgaacgaa gtcatttcga accgggcgac
    75001 cgaactgctc ggcggcgcca gaggcagcaa actggtgcac ccgaacgacc acgtgaacct
    75061 gggacaatcg agcaatgacg tcattccaac cgccattcat attgccgctt cggaaaccat
    75121 ccagcggcag cttctccctg ccctgaccca actccacaag gcactcagcg gcaaagcccg
    75181 cgagttcgac aagatcgtca agatcggccg cacgcatttg caggatgcaa caccggttcg
    75241 cctaggacaa gagttcggcg gatacgccag acaaatcgaa ctggggattg ccagaatgaa
    75301 gcgggcccag gcggccttga gcgaagtcgc cctcggaggc accgccgtcg gcaccggact
    75361 caactgtcac ccgaaatttg ccggcaaggt gatggcgctc atttcgaaag aaaccggctg
    75421 tagcttcaag gaggcggtga accacttcga aggtcagtcc gctcaggatt cgctggtcga
    75481 ggcgagcgga gagttacgca ccctcgccgt cagcctgatg aagattgcca acgacatccg
    75541 ctggctcggg tccggtccgc gctgcggcct cggcgaaatc aatcttccgg aaacccagcc
    75601 cggctcgtcc atcatgcccg gcaaagtgaa tcccgtgatt gcggaatccg tcaccatggt
    75661 ctgcgcccag gtcatcggca atgacgtcac catcaccgtc ggagggcagg ccgcgaactt
    75721 tgaactgatc gtgatgttgc cggtcatggc ctacaacctc ttgcagtcca tcgaactgct
    75781 ctcgacagcc tcgaccaact ttgccgtgaa gtgcatcgcg ggcatcaagg cgaacgaaga
    75841 gcgctgcaag agcctcattg aggagagcct tgcgatgtgc acagcgctcg cgccggtcat
    75901 cggttacgaa gccgccgcca aactggccaa ggacgcctac aagtccggca agaccgtccg
    75961 tgaggtggcg cgcgaacaaa acgtgttgcc tgaaaagcga ctcaaccaac ttctcgatcc
    76021 ctggcgtatg acccaggccg gtggcccggt cggcagcgcc ggagggtagg cgacgagggg
    76081 gttacaccag cctcccccaa agccgtcgac atcggggcag ctgaaccgcc tacgtgattt
    76141 tgacagcccg acaacccctg tgctaccgtg ccaaaaaatt ttgggttctt tggccgtctg
    76201 acggtcgagg taatcacgac ctattatcga tatacattga agggtagata atgagtgcaa
    76261 ccggttccga gacaagcggg catgtagttg ttgttgtggg cgcaggtcct gccggaatgg
    76321 ccctggccaa gaaaatggcc gatgccggcc atgaagtcat catcctcaat cgtgacatca
    76381 agtttggcgg gttggcggag tacgggattt tccccagcaa gctcaaactg cgcggcggtc
    76441 tgaagaaaca atattgggac atgctggaac agccgaatgt gcactacttc ggcaatgtgt
    76501 cagtcgggca gggcaaagac ctcacggtgg aggaactgcg gggattgggt gccagtgccg
    76561 tggccttctc catcggcgcc cagggcacaa aagccatcgg cgtggaaggt gactccgcca
    76621 agggcgtgtt ccatgccaag gatgtggtct accacttcaa tcgcctgcct ggtttcggcg
    76681 accgtccgtt cgatatgggc aaacatgtgg ccatcatcgg agtaggcgac gtcatggtcg
    76741 acatcgcaca ctggctcatt cgctacaaga aggtagaacg ggtgacggct attgctcgtc
    76801 gcgggcccgt ggagcgcaaa tataatccca aggaaattcg agccgtctgc tcgaatatgg
    76861 atcacgaagg catcgctcaa gaattcgcac ggatcaagga ccgcctggcc gcagtcggac
    76921 agaatgccga cgaagtcctg gcaaatttga ccgcggaatt tacgaagtgc gaaccgacgg
    76981 gaagctcctc caaaatggga ttccggtttc ttgcgtcacc aaaacgggtg ctcgtcgatg
    77041 ccaacaaccg cgtgcgcgcc ctcgagatgg aagaaaacaa attggaaccg aagggcgaag
    77101 acaccgccgc ggtcggactc aagcagctgt atgagttccc ggtagacagt gtcgtctttg
    77161 ccgtaggtga tcgggtcgat gagacggttg gcttgcccta caagaacggc gtgtatgtca
    77221 cgaacccgac caagaccgcc aatgatccgg acgattcgtt attccaagcc tatgatgaaa
    77281 aatcagggca ggtggtcgac ggggtgtttc tcgccgggtg ggcgcgcaaa gccagcgaag
    77341 ggctcgtcgg aattgccaag cgggacgggg attggtgtgc ggaagtcatc tcgcgctacc
    77401 tcacgaccaa gtcggtccaa tcagtcagtg cagtcgacca ggtcctgtcg aaattgcagg
    77461 cgatcctgaa ggaacgaaag agtaagccgg tggacctcga aggcctcaag gtgctggata
    77521 ccgtggagaa ggctcacgcg gcatcacctg aagccatcgg agaattcaag tttgcttcga
    77581 acgccgatat gctggcgcac atcgaacgcg ggcgcgcata acgcgcccgc tgccagtcag
    77641 ccgtcacccc atttcagttt ctcgcgtaac acttcgtagt aactgctctc tggaaatcgg
    77701 atcagcctgg tcaggtgctc ggaggcatga atttccacgg tgtcgccctg actcagggcc
    77761 acaccgacct ggccatccag cgtcgccatt gctccatcat ccttgctggt caacaccact
    77821 tcaatttgag cgcttgccgg gacgatcaag gggcgatgcg tcagcgtatg gggacagatc
    77881 ggagtgagaa tcaatgaggg gacggccggg ttgataatcg gtccccctgc tgagagtgaa
    77941 taggccgtcg atccagtcgg cgtgctgatg atcaacccgt caccgcgcaa attcgtgaca
    78001 aactgaccct gaatcgcaat cttgagttcg atcatccggg caagcgtgcc cttgctgatg
    78061 accacatcat tcaagacgac ccctcgcgcc accgtttcgc cgtgccgatg gacatgggtc
    78121 ctcagcatca acctttcatc gagcacgaaa tcattggcga acacgcgctc gagagacgga
    78181 tacaaattct ccagcacgac ttcggtcaaa aatccgaggc cacccatatt gactccgaga
    78241 atgggtatcc cgcgttcgcc ggccaggcgc gcagcattca gcatcgtgcc gtcgccaccc
    78301 agaaccagca acacatcggc cttactggct aattgagttt tctgaaaccc gccttgttct
    78361 ccaagcaatg ttgtcgacgt cgtatccagg agcacgtcga tgtttcgcga gcgcaaccaa
    78421 ctcaccacgg cctgcaccgt ggccttcact tcaggaaatt tgggtttcgt cagaatgccg
    78481 atgcttttgc ttttcatacg taagaccgcg tcgcgctgag gtgaggagcc ggttgaaagg
    78541 tgcgggattg tatcgggatg attcttttac tgtcaaaccg gcgtgcggct tggcctcgca
    78601 tctgcgcatc gtaaatacac gcgcttcacc agtcgcagcg cgtcgtttgc gattcgctca
    78661 ataccttgac accccaaatt gcggtagtta gaatcacatc atcaagtatt ttcgagcctt
    78721 tgacctgcat cgattcactc agctaaggct tctcttacag accccatcac cataaggagg
    78781 cgggatgttc gaacggttca cggacaaggg
//
LOCUS       DAOQDS010000007        78781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_92_length_78781_cov_15.810495, whole genome shotgun sequence.
ACCESSION   DAOQDS010000007 DAOQDS010000000
VERSION     DAOQDS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 78781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 78781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78781
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92_length_78781_cov_15.810495"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1616..7321
                     /locus_tag="PK589_02655"
     CDS             1616..7321
                     /locus_tag="PK589_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18524.1"
                     /translation="MTFHGRIESADGGVPKNGWDAYRLVFTSSAAVDGVPRSLDRSIT
                     IRGDGSYSADPGDPIPADASLSVVSPEGQILARRQFSSLVEDGTPYLKPFPVRPRDLD
                     LVIDPVDEPVDQRTRVSGRVFVAAGASSDHSAAAELAVRIFGASGDGARFAPIFTGVT
                     DRSGYFAGFVQRQRLTAAFGETGGGAALVRHTIHITPGNWLPEPVLLFIDAPGKTSDE
                     ENCGCTAPPPPTPGSAELAVHGEAFSTDLGAGCQDFTVPNRTLEEFDFFKIVRTTDPA
                     IRGLTLPDTVRNEIDLPQIASLAFDRGTLAQAVGASAIAVQTAPKPPTPELPVSSDLR
                     RNATPSVEGARTMARLATVVANTEAAAAATMADTGIADYVDVSPLQSSGVRRAAEWGN
                     SVDERAMQAALREAVAKIPPAAIKAALEDPDGFTPEGLMTLQRSVSAKALRDYLNWRI
                     KPVANRGELNEINLVDWDFTPEFYQATSIAHGHILHFKQEWRADGYSLGELVRSIPLA
                     PGQKKQIAVLEWDRADTAARTEATDVSEAVSATLSRDRDIDEIANVAFSESLRGGSRA
                     TTSAGGGGFGLAIGPLVIGGGGGAAKASSNAWSDNSRSLSGQTLNQLRDRVSQGASAV
                     RNQRSTVVETVGQSERVTATTEVIANYNRCHALTIQYFEVLRHFAVHETLASVQECLF
                     VPLEMTTFDDAKVLRWHDMLLNACRDRRLTPGFEAIRRLNSTEPLPPNRRYADDPIEE
                     MTGNLRVRISIARPKDPKDATVAVQDTEWRFFGFVLRVNPEVLYEVYRRDQVKRDKIF
                     RTEIAPQIAQAFLESLRVMLVDRDGREHDAGFDITVASRYAENGVMEVELNNTGRGPR
                     LTRAQIAGVSIQTDFELPEFSKVIVESASASYATERFSGSLFTGSVNDDVVKGDPAFL
                     SASQLSRAEERNQLAEMRRLRRRLLRHLNDNIEYYHRAIWLGMDANRRYMLLDGFEAP
                     RSDGRSVASVVENRLVGIVGNCLVMPVAPGFQLDPVLAEALAEEEDPQKALERLYKVE
                     AAPPRRLSVPTKGVFAEAMPGKCNSCEIIEEDRFWRWKDFPLPDSPSAISALSTASRF
                     AAPGSLRPTALPDAIVKFQALPSLPDPTGMAAALSLLGQDVFKDLTGLTNNQKNAMAA
                     LTTAMGTSEAFAGEAFKLAMAKDAARNLDRTLEQIKAAKKADLLTAEEASKATRDAIL
                     RSIAETPASSKDVVTTPEVEEAIKKAAETPGGSAKITREKGEKTEEVSFAVDESGLQI
                     GSATGHVREYPVEIWVEVPLVHDVVDDTTLDYSDSDRVDTLEPARQKEAVPAKLAGTD
                     ILVDRIGSAIDSDLMREGPSGRLQIRVNAMIGYPADPADNKKILQPQGKTKYGFVAMI
                     HGNSTAWKLEDGPVTDAPPITGSSLDVKTVPVTERGDYRGYTYLQKHLAELPRPIVSI
                     SFSHGLPNMLDHFVETRSRMLLAAIGQVVADAAQPGSLLGDTVDFDATGLMGHSRGGE
                     AVVRAFEQSKGMAGFTVTAICSLAPTDFLGASNNFKLSLPPREGVNYLVFYGGLDGDV
                     VGWRETTTADRGGNGFRLYDRTECDKAMVFIPRATHDRFNTVWAANEEVEWPTSKHGV
                     TLPGVPDRTLSKSTHEALAKEFIGGFFDLILNGNSGEQARFRNEVKSPTGAAISHQWQ
                     FATASQPLDTFTGTAPDFGTRTLPAGATIERFAPLTPVASRDEKIPHDDNACILATSS
                     AGTPAIVHEFTGGPPSSFDTIRFRLGRLYPSLQKSDVDTAVTLSPASVDVAKLRALLK
                     TKQEDEINLVKPPEFDVILEDTDNKTARIAHASLLLGQQNGWARPEAKVPVEVVKLPS
                     GDIFAVAKDCTLMSLQTLQIKTVQLVGIDPSKLRKISFEFKTPATAQEVWLDSIDFVL
                     T"
     gene            7331..7621
                     /locus_tag="PK589_02660"
     CDS             7331..7621
                     /locus_tag="PK589_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18525.1"
                     /translation="MNFKSLTVLAVPEEVQTTTGFVARVFAEIESVPATGLPLTAEIG
                     GQRMEALGVVQGLEPLLSGFVRVVPQPGDELVIKIGEVTIPTGLTVSDPAIA"
     gene            7636..11883
                     /locus_tag="PK589_02665"
     CDS             7636..11883
                     /locus_tag="PK589_02665"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HNJ18526.1"
                     /translation="MKLNGQLTGARSGYAGHQVEIFIPALISAETGARILAPTFVRVP
                     VSDDGKFSFDVKSGLTLSSPMEVAVRAPSGERLAFLPQPTLSDFTIKVLPHTAVPVAP
                     ETSPTDPALGPLKTRINLHDKAEQDRAANQLVSVWGIHPVAGGTEPVQNVLGASYADP
                     RGVAVVEIPRAAWRSLEIEVPGHRGPNKRFALKQTPDGLPVESTDILVDLTPLPSSTE
                     EAQCDCKESTLPRTPGHEELVGSGVFAADPVAGGCVNLAVPNRTIEEYNFQSLVRTTD
                     PFLFTSKPPRPRPLPPLSPTALTLAAELAYGVNALDTQLGQAAAGVTGTQPSTRGTAV
                     LRNGEAALDALRLESHTYRSTGLAAVRAWRETAAKDRVVNAIMERASSLSEDTLRHAL
                     ADPDGFTPVALMTAERHRAFEQLGQASETTPASGRTPIAAGNMPQWDAQPKTYQAATI
                     AHGHLLEWRQVWRADGYSLGDLLYSLPLAPGQKRQIVVVDWDRRETGMRTESRTVAES
                     FSADLSRDRDVAEIVNSTITESIRAGSSTRTWGGGGGLGLGIPFSGGFFGLGVAGGGG
                     GSDSQAWQNSARSLSASAGQDISDRTQQAATAVRSQRATVVTGRQQHESVSVTAEVVA
                     NYNHCHSMTVEYFEVLKHYRVDLELASAQQCLFIPLQMTRFDEMKSLRWRDVLSQHLR
                     DRTLAGGFDAIERILTGYVDSDFPPARYADEAMTELWGELRVELDIRRPREPRENEDI
                     NDYLNNNWNTWNILFGPGAAARAYDQSVRERALADKIFADELAPRIARAFADTLQFEL
                     CVDVNGTPVYVPVTADFTMVSDYRAGREHLIAFRCVLPSNQQVARARIKAVRFSTTLG
                     EITPGSRSILRYVHVSYRNRYRSFALLSPRRVRDEIKAGDAVFLATYALAKWEELNPR
                     LEDLRLRDRLLKHLNEHVEHYHHVIWWLMDPGRRFMLLDGFIAPDSEGRSVASVTENR
                     LISIIGNSLVLPVAPGYVLDPVVKNAEDPDRPIDLLEAYRPAIPFAPKRISVPTRGVH
                     AEAILGECNSCAILDNKRFWDWASEPIEEEPTPIQETSTETRRQTPPDTEPTPFTAPI
                     VGIQNVPEAPAPTALTDFTKLLGQKDLFKDVTGLELNQANALGAFQRAMQTANFFGKM
                     AAAGAKASFANRQSENVMKKVTEAVKNGDMSKEDAKTVIDKLIGSMNGTGGESDKPLT
                     GEKAVKDALNKVVSGSGKVNVSSESGDGKQTVEAKFADKNGSAQSTDVQIDGVPAVDQ
                     VKSQGCWAAALTMLISFQRKATMPIETVLAEGGPEYVEKYQSNGGIKPSEIAALKDTF
                     KLRDASSGAITLASLSSRLSERGPLWIAVDDDPSQQFSAHARVITGLKGNMVRFIDPD
                     GGVEGEEEFVVLVRQLNELSKGVRSAFGGYAPLILSL"
     gene            complement(11896..12318)
                     /locus_tag="PK589_02670"
                     /pseudo
     CDS             complement(11896..12318)
                     /locus_tag="PK589_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611376.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            12490..12945
                     /locus_tag="PK589_02675"
     CDS             12490..12945
                     /locus_tag="PK589_02675"
                     /inference="COORDINATES: protein motif:HMM:NF039390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18527.1"
                     /translation="MIIEHLTYNPLEETHTIDIFAQQKKEDLSKGFKANRHIYDYVLT
                     DSSNERTFVGELDASSEVVVYAKLPKAFHIPTPVGNYNPDWAIAFEAGQVKHVYFVAE
                     TKGSMSSMDLRKIEETKIECARKFFAKITSDQAKYDVVNSYGKLMELVK"
     gene            13101..13496
                     /locus_tag="PK589_02680"
     CDS             13101..13496
                     /locus_tag="PK589_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HNJ18528.1"
                     /translation="MEQCVQSDPAHAIGTAKELIETCCKTILAQRGKSLPGKPDIPEL
                     TKATLKELKLVPDDVEDSARGSDIIKRLLQNLGTIGNNLAELRGLYGTGHGKHAHTGT
                     LTPRHARLAVGAASTLATFLFDTHRERAS"
     gene            complement(13670..14410)
                     /locus_tag="PK589_02685"
     CDS             complement(13670..14410)
                     /locus_tag="PK589_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18529.1"
                     /translation="MEQSAARRGPIFAEGASGRSRPRSLIAAISEIQELAVVEKLGTP
                     LPEHLILLDRSFAYTEVGVHSVVVDGLINMLGVPMGLGVLGSLMPIFGDPDPSLFDQV
                     FAQLIGVTYGVGYAVMIGHNLGSCYIGKVCKRAIQDIYQSMIVANIIKVILLSVFFNF
                     MYQLLTPEHVQEGVKLLWPILRGVMSDQMVVGVQDWVISMREVMIPSATMVILVTIAT
                     LCVPYVYFVIGGREAAEEKRRNRLYDVC"
     gene            complement(14413..14631)
                     /locus_tag="PK589_02690"
     CDS             complement(14413..14631)
                     /locus_tag="PK589_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18530.1"
                     /translation="MDPETFIAVSSWVNTTLLAGVPLRILASATLAATFWFGAYKQRI
                     ALGFLFFMLTILLAYFRPLINLIGSGSM"
     gene            complement(14644..16023)
                     /locus_tag="PK589_02695"
     CDS             complement(14644..16023)
                     /locus_tag="PK589_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNJ18531.1"
                     /translation="MLGRLWGAWRGGSARIDAGETTQRVVPSQIEITLEDLAKDVWLP
                     LNLDFIQQTGELVEEAEPTVSPVEHAAAELEETIAPASLASPSAPALVVGPSSAQLTT
                     PPEEVPQAATPDPATSPRPVSSGRSSALIRDFIMPYQPVIESQRALEPLLTVVKILEE
                     YGDCPSIVTAGVERDEECTDLYSIRDTLAKVTLRDHTYRVTELALAVLKQTYRDGDLM
                     IPKVLVAALGHDLGKIPRFRATAAHAMGDHPVVSALKLQECFAGTSIPWFSEVLDAIK
                     GHHRIGKDRLGVILRQADGQARVKEMILSTQEMQEKPLDSWCAGSEVLAIVAPRINRP
                     LKGNKWAAFSLKGVVYVTPDALLEAAKELARQKKIVEMGLIRSTDREDTLRRLVKILG
                     AADLLAMEIGEHFYGRPFDIFTKKAGIKQRGYFVPVKLEAFRIAESELESRKVAFMQL
                     VTEFQLGRG"
     gene            complement(16025..16474)
                     /locus_tag="PK589_02700"
     CDS             complement(16025..16474)
                     /locus_tag="PK589_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18532.1"
                     /translation="MSNREAEQCATGNVSRARWAFDVTPGREKHVVLQLRELGCTSVE
                     RPTKGYVIAQCDPTAVPVIKGLPGVVRALPLVDTDVFEDLSNEPLPAKVSDLVQVSSG
                     PYAQMTGIVRALEGEDVLIDVSLMGRVVRVRAKAAHVSVMPLPEPWR"
     gene            complement(16516..17040)
                     /locus_tag="PK589_02705"
     CDS             complement(16516..17040)
                     /locus_tag="PK589_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNJ18533.1"
                     /translation="MVSLVVLPDSVGAEMPPDRFWYVNHSCVVAAANRYAVTVQILEA
                     IILVESEGDPHAVNVNRDGKGDRRGPLSLKQAKALVAELWKTGANFDVGIAQINSVHM
                     RHYKIDPVHFLDPCINIQWAAFVLRQKINEYQETWTAVGRYNGSRNIAGYSWKVYRAL
                     ERLAGIRRARGLLR"
     gene            complement(17116..17760)
                     /gene="traN"
                     /locus_tag="PK589_02710"
     CDS             complement(17116..17760)
                     /gene="traN"
                     /locus_tag="PK589_02710"
                     /inference="COORDINATES: protein motif:HMM:NF018666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HNJ18534.1"
                     /translation="MTTAQSAAETLPPDSITCGSQNSSVPFCVDTNTPPNQNLALAAT
                     WLSMTKNVEEDWDPDRLRIFTGKRSTCDFSTIGRSLINCCDSDPDKLIGKCSDEEIAL
                     ARDKQDWKARLIGTHCVDKGPFGVCLRKEEVYCTMKSELGRMVQEQGRAQLGMTWGSA
                     DAPQCDGFTVDQFSGLNFQAMDFTEWYKNVSANIDPAVITKEMATKICTYTGTC"
     gene            complement(18145..18636)
                     /locus_tag="PK589_02715"
     CDS             complement(18145..18636)
                     /locus_tag="PK589_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNJ18535.1"
                     /translation="MKILTVDVIPGHAYRYFQALIQIETVGIASWRDAVVRFTDFWGG
                     RAKTYDSPIVHATQSMVDRLAQSAASLGADLIIGFDIQIHQVTARYWGWGMAQIVMRG
                     TPIQFTDRHAMGTIFAGESAPFVASGEPPQHPADGNFQGEAAGMAEMPVPSLDTARFG
                     TRV"
     gene            complement(18643..19620)
                     /locus_tag="PK589_02720"
     CDS             complement(18643..19620)
                     /locus_tag="PK589_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18536.1"
                     /translation="MWPRMAVVGLAVLSGCVSAGEYQDLETKYQHQLAVKEVMETEHD
                     ELSHRVDNVSQVYRHVAQEQMGLKAQAETLQGEILTVKGVVKDTQSQYEAQRAQFAQQ
                     TQQLSGVDARLQNIAGKIETLTETTVALANRFERVELSIGKLGKSLQARGGAPEVSKT
                     RVQVGEGKSGGAQKADPVAKAGSPNMEGSAGAGAVGSGSEPGGTSLAAAPSPVAPTTA
                     PAKPDVPMVVSPKVVEAKFEPAGSKPDADKGWLRRFLDGFRGGNGQVAEPVSTPPTAG
                     SVGKASPGRPLPPSVDGEVMAAPMNTLTISTEGDAPKKAEVSPIDTEKR"
     gene            complement(19635..20183)
                     /locus_tag="PK589_02725"
     CDS             complement(19635..20183)
                     /locus_tag="PK589_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18537.1"
                     /translation="MEDPNSILLLPQTKAGMACVSLLYMFDKVVHRLRRAAGSQLSVE
                     QVRNELARVEQWVARAKGLLRELGVGEFELLPQELYPDLKERSVMAARRNARVITPRV
                     GDTVRLVPVIRALDSALNSIRLRAEDLSPYVKHFQAVDELVVALHDVIGHVARVTVTE
                     YEPRGAVKAFLQEGKESQSTLS"
     gene            complement(20322..20951)
                     /locus_tag="PK589_02730"
     CDS             complement(20322..20951)
                     /locus_tag="PK589_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18538.1"
                     /translation="MARVIRMDPGHSAYLLWADSIGRYAILPHQHADRQYKVRDSLMG
                     AVYKVGDEFPMLSQRSMHYFRRIAELVLAPVLADDHVHIKRVATSRAASFVKVAVDTY
                     RSGEDVIAACKPLLYEFRTYTSRTIALVKYHSELESYIREALAPAPSRSVRRVDVYRE
                     EHQARVIVEPGTIAQFLGRKGMNAAVASKLTGYGLVLVDEGDLGKVGGA"
     gene            complement(21072..21338)
                     /locus_tag="PK589_02735"
     CDS             complement(21072..21338)
                     /locus_tag="PK589_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18539.1"
                     /translation="MTPTVHPKPSLWSDGATRGGSTAVVEVMSRDSLNVCNVVGVKTG
                     AQDTPVTNPSRITLIVRQDDSDVSATRARTFSLDDSRSGRGLFR"
     gene            21419..21607
                     /locus_tag="PK589_02740"
     CDS             21419..21607
                     /locus_tag="PK589_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18540.1"
                     /translation="MALTIRSGAADQETAILEFVDIQKAERIRYDVLHFSRDWTEDAR
                     MFKDYLQTIFHAKYQSCS"
     gene            complement(22042..26508)
                     /locus_tag="PK589_02745"
     CDS             complement(22042..26508)
                     /locus_tag="PK589_02745"
                     /inference="COORDINATES: protein motif:HMM:NF019530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HNJ18541.1"
                     /translation="MWTIYTFGNGDVLTQILLGIRIMMTTGRFESLMELALLVFAMFG
                     LVYFVFNRRFLVPLVVGAFFILFVSVRITVDVSVEDQVNPDVPDQIVNNVPLAIALPA
                     YISGQVGYYTTQLVEASFPMPVQYTQGQTSFNRPLFDLQKVLNARLQDSDLARNTDTY
                     FMMCVFREVGLLQKNLGTLRNSPNLLNDVGAMNNALTALGYTGGTLEAAPRVCPDFYS
                     SLILPGYTSGQPDYEGAMRHLRTQLQLDPATGNEEGLTEDIRANLLLSVGQSARDMIN
                     NVLLIHAWKNAESADAKRRAATADTVLLQQEAIQKDLKFQSYSKSYVAQKVVPLLRTI
                     VEGVVYIMTPFLLVLAISPGMSRLMGTYWRLFMWLQTWSPLYAVLNFVMFWEARSRLG
                     AILSSNGVGQSVNYATLDRINEHVAMLNSTAADYVWLVPAIGFGLMWGGSQVLGSILA
                     GSRTAETVAGQVGGQFSRGLGANLEEGPSYRHEQRGAQFGEMIGHDVEPQMTVPAGPG
                     GFRVDSQSGTSEIHDSQGGITSIGANGIQNYKGPEGQYAMDSKGHLLSGVFRHKMTDP
                     QSGKQVDAQTSVFGSMVEHRFDTMGEDGARKSVLVQENQDTGELVARTEQSVRKGLSE
                     TRTIADDGSQSFSRSGNVPLFLEVAGETRPDAGFVQMMGERGPGERADTITQSMFQST
                     MGAKQSYTGRLVERDGQFHFLATEGGAIRGFDNKDTEAAVHRESFVSPDGSNYFVETG
                     QAQQMVLGKDGVTRPMEGYVRAFGSVDRSDPAHPRDIYSSYSLTSDEGGHAHTVTGTL
                     GTDASGRQFLVEATEHFESGTSGVAHDTQTVGSYTLSGTFTALSHGEANQPGVPTAFF
                     GSAIDNSKPGSPAFQANALLYDGQVSFFDGREGGTKSISGERNLTDGTHLSGQFTQLN
                     TMGQDGHQIEMFHGYAQQPGAPARDVTGLIDNGNLVAFNGTSGSHARDIVEKGSGVSG
                     RQYGFQDTLMAPGGTHSVSTFEGVSVDAQGRAFKNSSVELDGVRVYSDQMRGSRQMTV
                     EFEQSLRGYNDEVQSIDKESGWRRINDGHRDMDVYANLYYGPTSSETGERKLLGGTIT
                     NVTDNTYSTFMKDGEGNEVWGIARSLEDPNSEAMSGSFDQTLKVVAAKNGAVDTQVLG
                     PMKSDGGQREVLYHSVLGGLDSQYFNNERKSGVFTVPDPMHPGQTKELQGTFYYSPQS
                     GQLIASEVTDVQTGKVGQYRTDAQGQEHFQVVEYTQSPDGASVIASTRELSKHEYAAH
                     GFAVNDTLDSSGNVLSSSGNKGADFHDFNRVQFHHDSGANLTITQMAFAAKGTDITNP
                     TQTDEAIAYGLEFGRIGMDGASRYLILKGRMGKFGGGAAGGTPFQSGGGPLPDGPGGG
                     GGPSGLLDSHQNMGAWWKEYSGVMDNAISFARSSVEHLEKTGRAQTMSPEELQNFAAN
                     AAYNAVRTSAIGMLGEEASNAFGTMPKWQVGQESLLKVERTQPSAPSTSKEK"
     gene            complement(26557..27891)
                     /locus_tag="PK589_02750"
     CDS             complement(26557..27891)
                     /locus_tag="PK589_02750"
                     /inference="COORDINATES: protein motif:HMM:NF017892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HNJ18542.1"
                     /translation="MRCKRILVGLCMWAVLAWPPLVQAQSMNDALTNMFSSWGVGITS
                     PPGAYESQRRGYFSGGGVSVRLWQDPMRLWSIAPPRLSVGCQGIDVYLGSFSYGKLDR
                     YVQLLQQIGTGAVLGYAFQLAMKAICEDCADVLNKIEAAARALNAAGRIQPCQTGIEL
                     GKALAGNEASKQKLASRFGDAWQKMKEAGGAIGDVFEDRDAVKNQSNADAASALKGTE
                     YDVTGNLVWDVLTQAGLDQNLIVMVMSVTGTIIVGNDGDVQTRDPTMTFEQLVDSHLG
                     DVVKIFNCGGDSECLNPTITDATDIEGFESRVYTSMSNLIEDLYSGSAISAADQQIIN
                     MTPVPILTMLADYGRPRDVGNQIARLSSEVVAADMGYQWVKWAASETSRNVSYIQKVK
                     PEWPGNLQDFQGRLHQVLRGASDSLAKRQRMVNNINQLMQLTKTQGEMRSLR"
     gene            complement(27902..28762)
                     /locus_tag="PK589_02755"
     CDS             complement(27902..28762)
                     /locus_tag="PK589_02755"
                     /inference="COORDINATES: protein motif:HMM:NF025105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HNJ18543.1"
                     /translation="MSVCSMGGPTGVARAADPPGSKTFIDHPRQGWFFYQDPPAEAEE
                     DAEESPVSVTIDGLPPGAWLEPSKYRTWFKGLAMDGVKLTALPVTVLRELVSAKKERA
                     LDDPSVENVTSYIKVQKEAYDRSQRFTDAWQIAMYTDPKLDYASQHPTSTYGHSVESE
                     IKRNTEEQLLAGTADRVGLFFFFTSTCPFCQEQSKVLKVFADTYGLTVKPVSLDGVGL
                     PEYPQPAINNGMAENVGVHMVPMIYLAIPEENFLKPLGAGLMTNADLRERLLVLLRNR
                     GLLGERPSHG"
     gene            complement(28774..29862)
                     /locus_tag="PK589_02760"
     CDS             complement(28774..29862)
                     /locus_tag="PK589_02760"
                     /inference="COORDINATES: protein motif:HMM:NF018536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HNJ18544.1"
                     /translation="MTRSSMRGIRQVFLLVCLSCVLIGVATLWPSGTAEAVCTIGNPV
                     LANSLATTCWECMFPISLSGVTLFNPGEDFTAPAVGPGRAPFPPQLCGCVCLGPICIP
                     GLPLGIWEPKNLVEAVHDPLCFPTLGVSLAGGFSYIGRGMATTQMDSELNYAFFHVHY
                     FIFPLWAIVGIGLDIACLNPTGIADEIDLAYASEIDPLWDDDLISLILFPEALVLANP
                     VSLAACAADSVAAAVGFPIDPLFWCAGSFGFMYPPTGHVSGADGDLKPAALSMTRLLA
                     KLARAGTEFWTSANGPLLCDDVTTALIVKSQYKFQLLWPIPNTGIPPPCCQPLGRTLM
                     MWGLGKIVPAVGEDFVWLLWKRQNCCLL"
     gene            complement(29855..31051)
                     /locus_tag="PK589_02765"
     CDS             complement(29855..31051)
                     /locus_tag="PK589_02765"
                     /inference="COORDINATES: protein motif:HMM:NF021209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC family F-type conjugative pilus assembly
                     protein"
                     /protein_id="HNJ18545.1"
                     /translation="MKGSSVIACMAVTVVLSLLSIGSPALSESTGRPSKPSPEEMAQD
                     IARYSRQALRQGRPQESPKEMRRDGLYLLLSFSLPDDTLKDYLREAKLLGAKVLLRGL
                     VQESFKVTQDRIKRVLFTGERPDDSLLVGIGIDPVIYRTVGAGEVPALVFVKDEKFMV
                     ASGASSVAHLLALLSQEQARLKPWGEWFERRHRGFLQGGPTEEAPPLLPTIERSVKVW
                     ADARGADIAERDMIDVMKERVANANWPDIQRRSGDALKRHFAKGPGLALPHVEEARVA
                     FVDPTVEYPEDIKDPTTGTVLIKSGTKINPFDKVRWIRTLVFFDGTSPAQMGWVQQYL
                     REHDPKFVKLIISDGDVQKVMEQLHQRVYWANPLLVSRMGVGAVPSVVSQLGRNLRVE
                     EVAIHD"
     gene            complement(31097..31675)
                     /gene="lepB"
                     /locus_tag="PK589_02770"
     CDS             complement(31097..31675)
                     /gene="lepB"
                     /locus_tag="PK589_02770"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNJ18546.1"
                     /translation="MKPFNALSLLNVLKRAPTILEWLDRHRWVHVGLMLFCGTIIAAG
                     VIAPWYEVAARMSGSWPHHNVFLIQKGQSVGRGSLVAFIMQQDYAERVQPGRLHRDYA
                     DVGKRWMKRIVGVPGDHILVQDNEVYINGQSVGRGIGIDRYGQRIDLATLPETIPEGQ
                     YYVALPHPRSFDSRYYGLIRQADILGTVTPLI"
     gene            complement(31605..34118)
                     /gene="traC"
                     /locus_tag="PK589_02775"
     CDS             complement(31605..34118)
                     /gene="traC"
                     /locus_tag="PK589_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HNJ18547.1"
                     /translation="MSTILEWKAKQLVERTAISDWLPYEAWDDTNQVYRLDDGRIGVV
                     FASKPLLGLSEESIQKLTNLFESDLPIGTAFQFMLHASPIIEPYLDHHVVLAQRGQAG
                     ERYVADARKTAEFYLHARDTQLTSNPPLRPRDFTVYVSVAFPTQETTAQGWEDERIHQ
                     AVSGIEGQLNTLGLLPERVGPLQLLNVLHVLLNPGHSWESRPPAYSDQLPIRDQAVRL
                     DTAATLRTKMIELDGKFLKTLTVQSWPVQWHGGNNRELIGSLVRFTDQITCPFFLTLN
                     VLKFDQGKAKGKLRKKHVVINQQASGFLLGLIPSLRFKLDHFNGVQAQLENGRQLIGS
                     YFQVVLYGDTPRHVEEQTGTLRALYRAKDITLQEDHFIGWKIFMTGLPLGLPSDDKAL
                     IDGLRRMKTYHTEVPAHICPIVADWKGGGAPVVLLTSRSGQLITMDVFANQQTNYNMA
                     IAATSGAGKSFFMNNWIKSYLGIGGQVWVIDAGFSYVKLVELLKGQYIQYGANAQRLC
                     FNPFSKLVHWGSSAGGDDDPSDRADELATLKALHAQMASPSRPLSDVELSFIEEAIMR
                     VLEVEGREGCPDSVHRVLRGMTDPRARDLARVLASYAKGGMYSHLFNGTNNVNFENDF
                     VVLELDGLNEQKQLRSVILLQMQMNIQAVMYKKENRGRRKFVILDEAWDLLSQGGNTA
                     AFFETGARRVRKSGGSMITITQGINDYYDKMRDVGRYLLENAEFVGLLKQKTESIAAF
                     RNNGRIVLSEMEYRLLSSVTKTTEYSEIFMITPFGRGICRLTVPRETQLLFTTNPDEL
                     ALIDRVRKEGPKELSIDEAIQCIIAAERAQAGADNPRVA"
     gene            complement(34143..34805)
                     /locus_tag="PK589_02780"
     CDS             complement(34143..34805)
                     /locus_tag="PK589_02780"
                     /inference="COORDINATES: protein motif:HMM:NF021212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HNJ18548.1"
                     /translation="MSLCVLFAGCGGYESNFSCKGYPDQATCESVSEAYEKRFAAGQK
                     VTQYTKDQKDASHDTSGNSGPGQAPSPMVFSGKPDSAVGKPVITPASALRVTVFPWKD
                     TKKRLHDQSRHYLIVDEPDFIFGHMTQGVSPGSSSWHGKDLYPRMGRMVEKRDGKRNS
                     SGNAAVNAMTENIPEAGQRAAPMPVNPLGSGGIMPQGFPQLPQSGGAANYLTDDGQLP
                     PQ"
     gene            complement(34917..35693)
                     /locus_tag="PK589_02785"
     CDS             complement(34917..35693)
                     /locus_tag="PK589_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HNJ18549.1"
                     /translation="MKQSYQQLMAGMLCAICVSVVCEPDLRAEPDDTVMPIKTVIEQK
                     FPGMKVLRVSKMEIPGWLLVESDSDRVENFMYVHDSGRYVLSGALFDIEMGRNVTQEH
                     VRDRQQALLAGMDASKTILLSPMQPKLDRPLLVFDDPDCRFCQQFHPEVQKLVEAGVP
                     VAVVLYPLVRTHPDAYRKSVAIWCASDQAEALGRALMSKPVVGEAGSCSHPIDDNIKL
                     GKRLGVHSTPMVFLPNGQSFAGYRPASEVLALLQLEMEQK"
     gene            complement(35715..37001)
                     /locus_tag="PK589_02790"
     CDS             complement(35715..37001)
                     /locus_tag="PK589_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HNJ18550.1"
                     /translation="MASIKPTESAILNPGDPIRPEKPAPSKLLAGVVLVGGFIVLSMV
                     LQQFRGNQPGIVRGKSPVKAVSINREATDKEIYMKDAGARIEALEQQRDQMRRLLEQD
                     GRDKTEMKTQLSDLQKRIESVARSGGGALAGTKSGALPPLPPPLSANPLGGASATPVS
                     TMPAPKGPIPVQSAPKPTGGALPAPGVAGGATEAFQVFRPETVPGAKKHEKKLWLNTG
                     TIIPVKLLSGMDAPARSTGAQGGGAAVNSPYPVLMMVRDIMKLPNEARIDAKDCFLVG
                     EGLGDLSSERVMIRGNAISCVKKNGEAVDIELKGFVSGEDGKQGMRGRVVLKEGALLG
                     RTLLAGFVSGISRAFMPYQSGFFVAQSPSQAFNLPPAGPVAMSGMAGGAGRAAELLAR
                     HYSMMAASIFPVIEIDAQREVDFIVGTGRELEGFFQ"
     gene            complement(37022..37795)
                     /locus_tag="PK589_02795"
     CDS             complement(37022..37795)
                     /locus_tag="PK589_02795"
                     /inference="COORDINATES: protein motif:HMM:NF018311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HNJ18551.1"
                     /translation="MTSSVYRDTKHRAMAMCVLGLLLCLSRAGSAGAAVVVGDEGSTP
                     LTLSGRDLNVLTFPAPITMATSSRADLKAKPEGRNLIVQVKTGPADLVVMAGEQTYVF
                     EVVVSASLGAQTVNIEDRRAGTVPYDSDPARQAGDYVDGLLDTLQMAARGALPKGYRQ
                     TTMNEERYPKWLELKVVQGVEYRGPKYRVIVYRLVNTSGQKYTLREQEFLTGTQKAIA
                     LNREVVESGEEVVVYLLDDAPSEPPKPKPEQKVDPDAKG"
     gene            complement(37806..38387)
                     /locus_tag="PK589_02800"
     CDS             complement(37806..38387)
                     /locus_tag="PK589_02800"
                     /inference="COORDINATES: protein motif:HMM:NF017152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HNJ18552.1"
                     /translation="MTIELWKSKLHNAQVLNLVFAALAIVLAVALVVNGFFRQNVAVH
                     VVPINPTQEYWANAMGGDEYYKKSIAMAMLPWIANVTPASVDFHHRQLLQWVPSSHYG
                     LMSESLGAEAVYVKTNNLSRVFFVTASRVDGDMAVFEGIERRFVGKSEIREEEREYRI
                     SMRWEQGRPWVVGLSAVHLDRSLQDGQSDPAKG"
     gene            complement(38393..38674)
                     /gene="traL"
                     /locus_tag="PK589_02805"
     CDS             complement(38393..38674)
                     /gene="traL"
                     /locus_tag="PK589_02805"
                     /inference="COORDINATES: protein motif:HMM:NF018836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HNJ18553.1"
                     /translation="MNGTFIPRYLDSLPQIFWWEIDELAVLFVCIFLGIVTKFLTYLI
                     VVGIVSTLVLSKMKNGKSEGFLFHWAYWSGVPTFQLTGAPHGTTREMME"
     gene            complement(38753..39073)
                     /locus_tag="PK589_02810"
     CDS             complement(38753..39073)
                     /locus_tag="PK589_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18554.1"
                     /translation="MGDGLRKVAGDIWRMSLLLGLMVLASFVVLAVPAFAGADATFGP
                     LVTLLTNYMQGSLGTLLALVAGAIGLGSAMAGRWAGMFTGFGVAAAAFYIPNIIPTIT
                     TGLM"
     gene            complement(39186..40031)
                     /locus_tag="PK589_02815"
     CDS             complement(39186..40031)
                     /locus_tag="PK589_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ18555.1"
                     /translation="MTGGRPTYMSWAGLACVGLLTVGGCTTRPAVPPTMSDPTRLCGQ
                     GYTFPTTVVSVQSGPQWLLSRPMGCPEPTPLTQVPNKPTVADLPPKPIKAGIHRLQGL
                     PTFSSGTLAARPGDMSRAPGSSPDKAVIPGPTGSPTTVVCRREPEVRHRRVSFAMGSA
                     DLSERAQRDLTELRIEEILYLRVEGYTDPTGSRETNQELGMARAEAVAKVLQARVKGP
                     VQVEVVGRGGCCFLPNHAESRRVEVMMLLQGRCADSSEVERRSEAPPVTPVVSAGATH
                     DSGKP"
     gene            40422..40751
                     /locus_tag="PK589_02820"
     CDS             40422..40751
                     /locus_tag="PK589_02820"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNJ18556.1"
                     /translation="MKPLRFLGNSLERLRQFPEDARHDVGYQLEQVQHGKQPTDFKPM
                     PSVGKGVEELRVWDDSGTYRVLYVARFADAVYVLHAFQKKSRATSARDLEQARTRYAE
                     LKRGKHD"
     gene            40744..41064
                     /locus_tag="PK589_02825"
     CDS             40744..41064
                     /locus_tag="PK589_02825"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNJ18557.1"
                     /translation="MTKTIHSFKSVWDALADTPEQAANLRARAALMRQITKVIETNGW
                     TQAEAGATCGVTQPRMNDLLRGRISRFSLDALVNMAAALGRPVRVELQPPKHRRVTRT
                     RRLA"
     gene            complement(41197..41595)
                     /locus_tag="PK589_02830"
     CDS             complement(41197..41595)
                     /locus_tag="PK589_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18558.1"
                     /translation="MRSSGQEPLVQRAEQCARSITYVAGELIRERFLLGGIVAEALER
                     SQYGDAVIVKIAYRMSTAVGKCIRPHMLYESARIYSAFGGQWERIEALRTGSDCVNRG
                     VPGAHVTTVLQQSVAQPVLESFLGVSSPVF"
     gene            complement(41793..43580)
                     /locus_tag="PK589_02835"
     CDS             complement(41793..43580)
                     /locus_tag="PK589_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ18559.1"
                     /translation="MNGYGSIQGSMQGKDVSAKVASCPEEMPQVVGVITSRTRARRGA
                     GDWIEIGRKHRAFVPDGIPVPSVGEVGECWGRMTANGADPVLMVSRIRSRRFEACASP
                     SELLGVLGVRRTPPLRFEEVCQLLGPLVEWLCCAGWKAVARGIVKGTLRSARAISADP
                     FLLYRRRRLPFQATVAAAQVLGHELCSDGHYKAAVMEVLARADEEGVGGISEAHLVAR
                     CAELIGLPADKVAPSVSIQMGLVGKFEQGLWFSPVVYFARKKALAMIRANQQDSGDAR
                     SSVQARSLRYRYSIVTGGAGSGKTELAKSLMTQVREQGGKVAATAMTGKAATLLGEDA
                     TTLHKLLGYGGGGYSVSTVDADLVLVDEAGMLTWHTLYRLLLACRGQVVLIGDPQQLA
                     PVGATPVMAELLTVLPVVRLGEEGSKGSLLVKVQVIRFASEALLLYQLRKIVRGYQDT
                     GVEWQALSPVYAGGLGVDRLNRWLQEIMNPDGPPCHGGFRTGDRVIVTKTRYDIGQRA
                     VNGEQGRVLGSMGDTIALRLDSGREVALRAEELRLSYCITVHKAQGSRYERVVFIIPE
                     RECGAFAVEERMQYVGRTRGREATVCMVY"
     gene            43740..44876
                     /locus_tag="PK589_02840"
     CDS             43740..44876
                     /locus_tag="PK589_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18560.1"
                     /translation="MLAELIHPLALMEHQEWEYRPRPSNAGPDHCIRKLTYHARHAPA
                     KSPSGRFLVVLDDSSWHEELVLNWISKSTFHLHSQQLELTCATTLFQGRPFPIKGHID
                     GVITDLLGIDRLLELKAIEHFTFTRFAEGNYPINHFVQVVFYLVGLAALNPHIDEALL
                     LIKNKNQSAFLEYRVRYDRDNDRLTVLEITHSTGARIYPKQDFVGLYQEALRRFEEVE
                     QYRDHGTLPARPYPNADNYHCDYCPYQFCWEGIEQVPVEGIAELRTELVPAVEEYLGL
                     QTELAALTPKQKRLDALKQQFKLELKTEGITRAEGHGYYIASSPYEQSKLDQSKLSKA
                     VIERATVKESYSRFSAGRLSKGIDTTKSKRRSPLKKPKPTTQIA"
     gene            44915..46249
                     /locus_tag="PK589_02845"
     CDS             44915..46249
                     /locus_tag="PK589_02845"
                     /inference="COORDINATES: protein motif:HMM:NF039495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18561.1"
                     /translation="MSDAEKPTFIPMNRINGISNRRRMPIIGKIRLGVKVSGPNGFYP
                     KETPHFVVPEEVAKVFGAQPNELEVMFVSNDPTVNCPQALRWYGEAAGLQCIGNNQVA
                     RRLNQDTWKTESRSCPCEKLESGECGPRANLIFMIPKVSSGGLFQIDSSSINSMETIN
                     SYFDFLALTLGRIAMIPLKLRRVPVTRRREGRPTTHYPLELRYEGSADETARYQQETE
                     TVLKKIKMLEIQEPIEINPVLDPGAHVVMEEELEDDPGPVAAEVPGDTPSETHDPTPP
                     AMFTPSTAAAKAPEPTPLPAGPLISVAQTNYLLRLTKALPLTEDDLRMKVRALTQREA
                     SGLIKRLIAQDFSYFTSSPGTPPITPANTGAVAESMTDPQFKKIMGLVISMDIQETHV
                     KSLTSGYTKRQASTLIDQLVGGDFSAFQEMKEEELPVLVTPESAPVEEKVEF"
     gene            complement(46351..47025)
                     /locus_tag="PK589_02850"
     CDS             complement(46351..47025)
                     /locus_tag="PK589_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ18562.1"
                     /translation="MVLVKDLLVRMRIAQNLTQTELAEKLGCTASHISIIEGGKSSGR
                     KKILPSDDMLRRIAQCLGGSPLDVQRRTYQLLVARAREVCSPEVAELLDDQILTGMPA
                     AFLERVKQDLSEYSDEELAGVTDQVQLGGRFKQVLSGIGRLSQAEVERLAQVLGQSSE
                     TYLVEAGYLSESARSLFGRNPNCVNILQVFGSLRPETQEELLRFGEASVSLPVSERMH
                     PPTAHE"
     gene            47410..48087
                     /locus_tag="PK589_02855"
     CDS             47410..48087
                     /locus_tag="PK589_02855"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNJ18563.1"
                     /translation="MSFRTQAQQLAIDSDNDSPLLKLFQQSKPIIHSIAKRYCNLDRS
                     YEIEDLEQIGFLAVRKATEKWDFSRGNAQFQTFLYRYIQKAYQSEFDGRDKLVEITDK
                     DDQYVKTLPYSTFIKEKRRLHSQGFKSRIFRRLVPLTTHEPPVVPDASAESLAQTDVP
                     GTDDVLGFTATDRFIEFSRKRARAIPDPTALDHDLLTTSEQSITGSSSGSGNHARQAR
                     KRRTYAN"
     gene            complement(48419..48616)
                     /locus_tag="PK589_02860"
     CDS             complement(48419..48616)
                     /locus_tag="PK589_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18564.1"
                     /translation="MMICMPGSLLLAVTSDDSVNVTSGQVVAATMKLPLELMTGDCGL
                     SAKDAISNLLSTGFWLGEVLG"
     gene            48603..49697
                     /locus_tag="PK589_02865"
     CDS             48603..49697
                     /locus_tag="PK589_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HNJ18565.1"
                     /translation="MQIIMIPTSQIRVHDEYATLFPPLTSSERASLKTSIQEAGIHDP
                     LRVERQPTDAASVQPYVVLGGHHRLSIAQELNIPEVPCIIVTTMELKVAALFDNIHRR
                     QLPDTLRRQMQDEEQRYRARLRERLIPEILDVLPMLPPEVQLQLTQSSHDTQRDFLSK
                     WVAQVQRVGIQPQSEPQHTSPAPVEPEAPDAPIPKQHKQLIRQMNQMTKDFERRIALQ
                     KKELEAVREHNISLSRQLREQSDEKDQLNENLASLQRQLTSENLDHKAERILRLQHKG
                     RRQGPAIEEAGPLIAGLLHTRKTVLVLQTYAQQMTTLTTPDQTTILDHINLTNQALGA
                     IEQVLPRPTDPLQSIPRPSRSVNGDRSADS"
     gene            49816..50445
                     /locus_tag="PK589_02870"
     CDS             49816..50445
                     /locus_tag="PK589_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18566.1"
                     /translation="MQCSSPESFNSVDQAHSADDHRSALRTTLSKIALYNFAAEHRLD
                     TPIELAERLILCVSLANEDRPLTRLELRQITMINPFTIDSALINLVQREEIHMLSQRT
                     SEQAVYGLTPLGHTVTVSLDSWTTSFYQYARCGSVAGVRDLVHQLQQAIRNMIATDSL
                     PIIRTCIACEYFSSAAHALDPQTAGYCRFLRTPLSIQTTKSAAPGIHTP"
     gene            50713..52824
                     /locus_tag="PK589_02875"
     CDS             50713..52824
                     /locus_tag="PK589_02875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNJ18567.1"
                     /translation="MSAYDTNTTSVFPLPLDPPLPAEEPSEPSPLPLSPRKLPDWLHA
                     HIHTPNDLTQERHSAVELNLATISPLIQNAQLNPEQLAVVTAPAEPTLVLAGAGSGKT
                     HTLIHRVAHLVTQGLPPDQILLITFTRKAAEEMIHRTQLLLQQHSLKIQGGTFHAMGL
                     IWLKHYGGALGLPPKFSIMDEDDAQGLVHLLMSKLSFSRRKGFPKKQHILALFSRAVN
                     TLRSIADTVSRDFPWLDAHTPQLVVLFRAYTETKWRQHRVDYDDLLYLVYKLLCLHEP
                     SRRRLGTHYQAVLVDEYQDTTKLQSEIVRLLGSHHHNVMVVGDDAQSIYGFRGAHTKN
                     MLEFQDAFPSVRLYKLEHNYRSTAPILTLANTVLQDAKDLYPKRLFTAKAGGQSPELI
                     ACYSEQAQSDLLTERILQCHDEGIPLHRMAVLFRSSAHSNNLELTLARHKLAFVKYGG
                     LQLTETAHFKDVLAYLRAADNPLDSNAWHRLLLLLDRVGPKLAERIIATMQEAPHPLD
                     VLDQVPGKAESTIRLLNNVIRTTQDESISLTDRLTFVIKHYEPILKRDYPEDAKVRIE
                     DLKPLCDLARQSTSLAAFLEDLTLNPSATSSSKHAHPDHHLVLSTIHSAKGLEWDVVF
                     VIWVLDGQFPPVRSWHEEQALEEERRLLYVAITRPREQLFLTYPTEHYHNASKQNLDS
                     LCRFLAPIPDDILPSYEMDQA"
     gene            52824..53414
                     /locus_tag="PK589_02880"
     CDS             52824..53414
                     /locus_tag="PK589_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18568.1"
                     /translation="MKPPSTFRFEFRTQCERKDRPFVARVTWDTEAKSLARGFYAIQI
                     TPVGRFGAVGECTFDAAIGDIVEVKRGETKDREYRGFYLVVPRGYLILVAIADEVDDA
                     LKVVQYVAGQASPQDLAKGHWRFESAESSWKGRPAPIDQTRMEPHPPRPVAQLAPPDK
                     RPAKPTREPAAPTSRSSTTRSVTRISRHTPPLNKDE"
     gene            53417..54532
                     /locus_tag="PK589_02885"
     CDS             53417..54532
                     /locus_tag="PK589_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNJ18569.1"
                     /translation="MTFRIANFHVRLHYTWLIGFGLITWSLAANYFHDTQADLPVSYQ
                     WASGAIATLLILVSVFLHELAHAVTARRLGYAVKTIELHILGGWTIFERELSSPRVEI
                     LVAIAGPGCSFLLTLLLYAMKADPLANFLYKVNLTLGAYNLFIPCLPMDGGRILRAVL
                     WTQTHSFAVATERTAQMSKQISSGMMAIGILGIIFQYQTLWLAIIGGILRMIAETAYR
                     TVQQSEQLTRPLQEVMIPWDRVVTLVHDTPLLELQDQFLRYGYRAYPTAEHGRITGLV
                     YYKDVRTDPKWLAPNGATITPFIRALSPDVIVHPTHTLQRALDAMLLSGSDQLLVFAG
                     NTCVGMVTRSMIARVQDAIGNPDSGPTLTRQREGFPI"
     gene            complement(54501..55910)
                     /locus_tag="PK589_02890"
     CDS             complement(54501..55910)
                     /locus_tag="PK589_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HNJ18570.1"
                     /translation="MSEQGIEQLRARNRGGHRDPIVDGKVLVGYGACLQDPQRREGYL
                     VIRDEDRSGHVGILGTTRIGKTRLMESIVEQDIRSHKSVVAIDPKGDIDLFSKVVQVA
                     AEAGRLDEVMLVTPIFPDYSNYLDPLANYYMEDELVSHIVSGIKVKDDYYLSIASEVS
                     QVIVSGLIKLATSRGQVPTISFNDVNQRVGYTELINFRDVLKVLNDTEELCGLIDRVC
                     HGPGIAEHFAKVSSSLRTMLSALTFGNIGRIIGKARTNEFITRLEQGKRVILVCHTGS
                     LLSPRASLIFGRVFVSMIQSLVGRLFASGRQLDPPLCVHIDEGHNVLYPGIQEIFNKA
                     GGANVWLHFYEQSGAQMSAEIGADLARSISDNINSWIYFRVNHAETAQSVEDASPLVQ
                     RPRSVVSVQGNVTLHLNEERLVLRDRVLHLPKRCFYMRTHGQWYKGKTLDSSAPYVQV
                     KFPTPAVNSPKSETPPSAA"
     gene            56073..56774
                     /locus_tag="PK589_02895"
     CDS             56073..56774
                     /locus_tag="PK589_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18571.1"
                     /translation="MKIPLHHSSDTQDVADADQALASHTVAIGRGTHRSLPNRRTGYT
                     QKATIGGHKLYIRTGEYEDGTLGEIFLDMHKEGAAFRSLMNCFAIAISLGLQYGVPLE
                     EFVESFTLMRFEPNGPVSGHAQIKMSSSILDYIMRDLAINYLDRQDLAHVQVTHDDLR
                     GDSVKPYAKSTSRHTPQPLSRAETAAPPTTLGLEEARIKGYEGDPCPECHQFTLVRRG
                     TCRTCSTCGSSKGCS"
     gene            56818..57039
                     /locus_tag="PK589_02900"
     CDS             56818..57039
                     /locus_tag="PK589_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18572.1"
                     /translation="MSAPQPIPPHTTWLQRFKRLAQLTASLHPEDPRFPIVMRLLEKC
                     DGYYRTDNHASFIETGKRIRSLMDTPPIG"
     gene            57131..58684
                     /locus_tag="PK589_02905"
     CDS             57131..58684
                     /locus_tag="PK589_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ18573.1"
                     /translation="MSEQYPLALDFRTARNVSVPLIAVNSLDPAATMRALAWSLVNVA
                     SASQAETDNEFGLHAFPILKWDIGHGLLPLNEEGKNWHAQYVGPSKQNTTINPAEALR
                     LLEKVPERALTFILNGHRYLSNDFFIQGLWNCRDLYKANGNTIVLLGIGFKLPPELAS
                     DVVLLNEPLPTAAQLESIIKEQLSAASLPVPDPATLGKAVDATLGLGAFTAEQEVARS
                     MQASGLNIDKLWDRKRQVIDATPGLSVWRGGNSYAQIGGVATIKRFLKQVLVSKRAPR
                     CIVWLDEIEKSLSGSTGQASDTSGVSQALLGILLSYMQDHQASGLLLVGPNGTSKSAI
                     AKATGAEANIPTIALDISGLKSSLVGSSEQQMRQALNVISAISQDKACFIATSNNISQ
                     LPPELIRRFGYGTWYVDLPSQDEREAIWTIYLAKFGLATDADRPSDHNWTGAEIERCA
                     RLSWELSIPLSEAAKYIVPTAISAKESIKALETQAHQTYLSANRDGVFDQNRDTPHHT
                     RPRTITLAQ"
     gene            58720..59037
                     /locus_tag="PK589_02910"
     CDS             58720..59037
                     /locus_tag="PK589_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18574.1"
                     /translation="MPCYLEQRVSVQLNVADFPLLIAAGTSLGWEHAINGGVLVFTTQ
                     QNQRITIAKGQATLEQRHETLITELKQAYAQTIIQTAAEQFGWTLERVEGAAQELRLT
                     RFS"
     gene            59058..59261
                     /locus_tag="PK589_02915"
     CDS             59058..59261
                     /locus_tag="PK589_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18575.1"
                     /translation="MQTDSIRITIETDGTIKLVTDEISLANHATAEDVIQQILTLSGG
                     ELERTQRLTHTHHHHHTDEHLHH"
     gene            59273..60184
                     /locus_tag="PK589_02920"
     CDS             59273..60184
                     /locus_tag="PK589_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18576.1"
                     /translation="MLNAESAFGSLWKNAFCLMLTYRKLGIQRRMDSATVLSIDTEPS
                     RVRVTKSILSCPEYMAITRLYSRVRTKLEKLTLPAGLRSGMYLIPVALAAEVDTIIAN
                     AEEELRQLVDVFLAQYDTRIAEEAARLKSAYRTSDYPDPDTVRAAFGLTYSYITFDLP
                     GTLETISATMLKREERRSMEKVNNMLEDVNTYLREAMRSLVNHLVDRLSPSDTGKSKT
                     FKSSTLTNLLEFLNTFEARNLGNDVELAQLVSSAKNVLSGVNSQLLRSDQSIAGVVQT
                     GMGTIKDQLDSLLVKRPVRLYNFDEAA"
     gene            60242..61297
                     /locus_tag="PK589_02925"
     CDS             60242..61297
                     /locus_tag="PK589_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18577.1"
                     /translation="MSITPDAIARARNINLIEFLISRGHTPVSRRPHHADFHAPYRED
                     RNPSFSVSQRQDGTWVWYDFGRNEHEGCKHHGDLIDLVQLLDHVTFAEALHRILHNTV
                     RSMATPSGPSLAPPTDKERIAHAKKLYYAARANMTPEREEELRSYFQTLALPYNQHLG
                     AVWMRLGDEGIPYVAFPLPTPNIHFMQGLMARALGEAPPTLMRVARGLKGPWILKRSN
                     APILITESIVDCLAGDELFGPSFTLCALNGLNTGERLQDYLQRLPSRIIYIALDNDAS
                     CAAMERPPHTSQPKKGPHVQKELVSLLTHMGYHVMEVLLHHNASVKDLHKLWLKRPQR
                     VSLLDLTKTGFHHSPVR"
     gene            61784..63379
                     /locus_tag="PK589_02930"
     CDS             61784..63379
                     /locus_tag="PK589_02930"
                     /inference="COORDINATES: protein motif:HMM:NF025085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HNJ18578.1"
                     /translation="MFSEDFYPTPGAVAAKMLQKINRNAVHFLEPSAGKGDLAKAILG
                     FGRTGSRYSSGSRHRVDVIELHPDLLKILQAHEELTVVGYDWLTYDGVSYYDAIVMNP
                     PFSEGALHLLRAWDFLHNGEIVCLLNQETIDNPHTEERRRLATIIAAHGSVEPLGPCF
                     RKAERPTDTPVVIVYLKKTTEDDRIHLWHTTDREQSINDDIGTPEAIPALRDTLGNMQ
                     HYYTHSLEEMFKAFAHIRKASLFMQALGTKLLPGSSIRDEADLKKILGMAQTNTTAAR
                     AEFARALRRSAWMHVFKQMDFQKWLDTKQTQELLQDLERDSTVPFTAKNIKGTLTNIF
                     LQRKRLFERSVWNVFVALTRHYKGNTTGDIGSGDGRSGWKTNDSYKVNCKLVFPYGCR
                     FWNERFDLWSTRDAGELYSDLDRVLAVLDGDKFEEIITVRDALECSFHNHGVGHRHCE
                     STFFRIRYFKKGTVHLRWKRDDLLASFNTTAAAGRQWIGTTCHNDQDSHASSPHSASN
                     TPHDWPSPAHNSPRLALTHSGDT"
     gene            63578..64042
                     /locus_tag="PK589_02935"
     CDS             63578..64042
                     /locus_tag="PK589_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18579.1"
                     /translation="MASNPEIQELATEIDECAAGAFSGGRTNNLTDLALSIGAIAASA
                     IAAILVADGKSFPLFTAFVAALPALATALQQTVDFRGRATWYFIKSAQLQELSLNLKY
                     GGIVEIREAARLFGEIERRMEERWVDFVKPVMPKNSHHAAQPNQRTTSGDKQ"
     gene            complement(64330..65049)
                     /locus_tag="PK589_02940"
     CDS             complement(64330..65049)
                     /locus_tag="PK589_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ18580.1"
                     /translation="MTASVDSSMNSPVKPISVVFADDHALVADGMTTILYGVNHKQRD
                     YEFAVVGQASSGRGLLELVKRVPADLAFVDISMPDMSGLDAVPKVREIRPDIKIVVVT
                     MHREPEYMTRALSVGANGYLMKSCSAQEFCHAIDEVMRGNAYVTSLLGSWPGGTLPGS
                     DDEEDQLASLGMSQRQREVVTLLASGKTIKETAFQLGLSRKTVEHHKAMFRDKFGIDT
                     DADLTKFAIKHGLTGIDWIRK"
     gene            complement(65046..66806)
                     /locus_tag="PK589_02945"
     CDS             complement(65046..66806)
                     /locus_tag="PK589_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ18581.1"
                     /translation="MKQIVGLGGPLPSGESYCVFLYANVPLDAKTKSLFQMVSGNISL
                     ACSAGDDQWWSARAKRGEGTPYSKEAGFAFAQGIYRRLLELNESFVVDQEKSYFEEVQ
                     ELRQSDLKMRRIADAVPGAVYQYVIARDGCQRFSYISRGAVDMVGYPADLIVSDYNAV
                     WKLVLPEDMPGIMASIEDAIRRGLRWAHEFRLRLPDGRVKWLRGDSLPEVPTADGTVL
                     FHGLLTDVTERRLAEAELRFTQFATDHAADAILWVGPDRRIMYVNVRAAELLGYSREE
                     LLNLSVPDIAPLQSRIDILNDLTRLKEGEVVQYATTFRRKSGAVFPIEVSMRCLEHDG
                     EGYVCAIVRDVSERKRAESVIQEGAEALRRSQEALRALHSQLLTAQEDERRRVARELH
                     DDLGSRLGSAILKAEGKLVLLENNSSEAAVLKNVTDELRGISDRVRTIAHGLHPSILD
                     NLGLSVALKKMVKEYGSWSGMKVVSNISMEFTEVLDREVATCLYRIAQEALMNVKKHA
                     GAQQVMIALARQDDWVQLSVKDDGMGFSPEAESRRTKGLGIVAMEERIHMVEGELTVQ
                     SGKGRGAVVVARAPYREREA"
     gene            67766..68581
                     /gene="traT"
                     /locus_tag="PK589_02950"
     CDS             67766..68581
                     /gene="traT"
                     /locus_tag="PK589_02950"
                     /inference="COORDINATES: protein motif:HMM:NF017619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HNJ18582.1"
                     /translation="MSPIIRRTLCSAGRAFVIVVVGIATGSIFGCAGHMTLSHEVNLT
                     SSNAIFLTPSEKKTIYLQTRNTSDNPNINFGELPNKIKAKGYAIVDNPDNAQFILQSQ
                     VVLCNKLKPGQTVDALIAGGFGGAIGVAAGSAAALSGASLSHIPILGAVGAVGGFAAS
                     KLSEDSIVACVVDAMVQERTKEEIQQSITTNSMPGPGVPQSQAGAFGFLNQQSVSPPQ
                     AGQVTQQIGEIRKGTRRFHQARIVASDQKMWLSVPEASKILSDKLEDSLAGLF"
     gene            complement(68884..70278)
                     /locus_tag="PK589_02955"
     CDS             complement(68884..70278)
                     /locus_tag="PK589_02955"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HNJ18583.1"
                     /translation="MDADFTPPSESDEGTGSSDGIPAELIFPGTTTDHGSLSVTTPDH
                     LLSEHILGSIPLWEAKGRPTGAERASYQKQIYRDVWRNSRLCKVSLVVYGSGDLGLPN
                     SIDLEFFRGFERWTKAALSRGEPFNQLVKISGRELLEASGKGLGGASYQEMDRFFLRM
                     AGTMIGAGRDWRQEGESGGSEKRPRSNKGIVFHIFQTVVLPGQVNAEGYVADKYEVEL
                     ATWYWMSLRSGNCIVIDHELFRDLHGSITKLLHQLLHNLFYLGRGTASQKYSELVRNW
                     QIKRHSALSLVKQQLDDAHRELLEKEFISRWEYVPIRTPEGKEFEIIWEAGPAWWATD
                     KKTREFREEMVGANHRELQQIGRELDPFLLIEQSDESNQEKDGQREASNARLLSVVLE
                     ISGKRKDPKVWEKWWKRAITSVPHPMIWRRIGEVKERRLSGQTINQGSYLLSLIRGDA
                     ARLGLPWATSDKVK"
     gene            complement(70704..71834)
                     /locus_tag="PK589_02960"
     CDS             complement(70704..71834)
                     /locus_tag="PK589_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNJ18584.1"
                     /translation="MTSKQVSKGKDEKPPLSKSKEAKPERQKSSHLADLEASFDRDAR
                     RIRPFALVGLDTQGGQPGPEHTTPPSQIPSTTFDSAVDGSASVSIPLGAEATASPVGP
                     KAVPIIAQEADHLRRLEEVVEVPLDRLVPSPDQPRSEVDPAADQELLESIRAYGVLTP
                     IQLRPVEDGKYEVVAGERRWRACALLGKTSIPALVRRKDRDRAAAEALVDNVVRKDLS
                     ALEEARAYQALIERYGFQQSELAERLGCHKSRISRALSILKLPESILNVFFGPESRMT
                     ATHAEALLPLAHDEPRLHAIARRASKESWTRDRIRQEIDRKPRINEGAQAVRFVERGR
                     GGDRGFMLTILFHSNKPHEIPMIEEALKQASERIQEFRDKLT"
     gene            complement(71831..72682)
                     /locus_tag="PK589_02965"
     CDS             complement(71831..72682)
                     /locus_tag="PK589_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNJ18585.1"
                     /translation="MPNVVAVINQKGGVGKTTTVVNLAAALSRLGHPVCVVDIDPQAN
                     ASSTLGKVSPYEARVTAATLMMDNKRDEDMTAPWYDTIEKEVRLIYGHVSLTKADREL
                     PRVYPTMPSIVLKRRLEQMAFGDEDIVLIDCPPTLSILTINALVASDYYLTPMMSGSK
                     YSITGYEDLMELVRDVVDSANPALKCLGILVTQHDGRKNVCKSMRGVIERRFGDLVFK
                     TAIPLAAKIQESESLKKTIFQLDRQSNAAREFMDLGREVLARMGLGALAEPDDMDEPV
                     EVGAGES"
     gene            complement(73142..73624)
                     /locus_tag="PK589_02970"
     CDS             complement(73142..73624)
                     /locus_tag="PK589_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18586.1"
                     /translation="MAMTLDRLQARTGLSRSTIHQALHRLADPDVDIVNVVHPGGGKH
                     PGLYEIHPCSYQKVLGLSAKRVPVRRRSKTAPPDGRSIENLLAQLSAEDRADLQVVYE
                     SLQAHERKMIEEEVRARFHDIGQNPDKTTFQQGLLFYLLKQRMYGRLRSKYPTLSSPT
                     "
     gene            73995..75236
                     /locus_tag="PK589_02975"
     CDS             73995..75236
                     /locus_tag="PK589_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNJ18587.1"
                     /translation="MATIPDQKEIHTVDESDSTSDGESPMTRLPRESGEGIKNPLRLQ
                     PGRDGDHVHLQATFYHYLLFCQANKAASTHRIYQSTLWAFYRWWRQERPFETLGPTLL
                     HAYKIWLCESRDRGQERLQARSINNYLAAVRAFCRWIHARYPLSWDPGREIPDERVDN
                     KTYQRLPLSPSQVTTLIGSFDDSLIGRRDAAMTYLMAKTGLRAIQIQRADCGDLECLE
                     IQVYQRVDGAREARGPAVARSQWILRTQGKGQQTKESFVNLMPEVYGRLQRYLEARGP
                     VLPREPLFARHHDKLTSHLAQVRSKPGGGEASTLAPNERLRLTTRAIQLRIAGAMTRC
                     GLRDVTRPGEASSLESRRRRQRRVTPHSLRHTAATEAARTESPFKVQAMLDHKDIRTT
                     QKYFHAIDRLEEGAEYAITRY"
     gene            75827..76072
                     /locus_tag="PK589_02980"
     CDS             75827..76072
                     /locus_tag="PK589_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNJ18588.1"
                     /translation="MDTIGAFEAKTHLSALLERVSKGETITITRHGVPAAKLVPVQGD
                     VGRLPHAALLEGMRELRKRVKPGAMPVKDMIEEGRRR"
     gene            76078..76509
                     /locus_tag="PK589_02985"
     CDS             76078..76509
                     /locus_tag="PK589_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNJ18589.1"
                     /translation="MVIDASVALAWCFPDEQSPYADKVLQELDGCSVLVPSVWALEIT
                     NGLLVGERRRRIGQSDILRFIDLLDALSPQEVSVPINAHFHALLPLGRGYGLSAYDAA
                     YLHTAIRTEAELATADDALEKAARTAGVPIMCTSHLRKPRR"
     gene            76555..76956
                     /locus_tag="PK589_02990"
     CDS             76555..76956
                     /locus_tag="PK589_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18590.1"
                     /translation="MLCCDFCSASIPTTKYRTHPFGLTDSSQVFGDTPIYTIQGLDHV
                     STEDLHPSHGTGAMMSPEWLACDDCAIFIDARDREGLVQYCVMRLSAQPNLESIDIAN
                     ELIPMYRKLFTAFFLYLESKEPSAASRSDLT"
     gene            complement(78524..>78781)
                     /locus_tag="PK589_02995"
     CDS             complement(78524..>78781)
                     /locus_tag="PK589_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNJ18591.1"
                     /translation="RGERMTTRGMRKQLKAAFREAGLAEPHVTALSLRLSGGALAYRK
                     GASPSELKKTMRHESIQTTQVLIDQVNRIRFGAERYLDDIQ"
BASE COUNT        17450 a        24876 c        20188 g        16267 t
ORIGIN      
        1 tgatttattg tggtgagtcg gctgggattc gaacccagga ccctcgcctt aaaagatcga
       61 aagaacggtt tttgacaagt gctcgctatt gcaacggttt ccccaattta ctcagtatat
      121 cgatcactta ggagcttcta ttgattctat gcgttccgct ccttatccat caattctgca
      181 ttgttttgtc acaaatttgt cacagcaagt ccgtacacta cccggatcag acagtcatgt
      241 ctacgatgca ataggcagcc tctatccaac cactattgat gacccatatg cattccgatg
      301 cggggatacg tcgaggcctg ctgacaaccg acctgcggtt ttcgaattga accggactca
      361 tcgccacatc aacaatttca acaagatagc tcggcaaatc acggcaaaac cgggcaaaca
      421 atccgcaaat ctgcaaccgg ttgcaaccag gaatcgggcc ctcaagactc gtctccagat
      481 aagggagatg agtcttgagc attcgtctta cccttccgtg tcgtcgtcgc ttgttcgctg
      541 gtctgatagc ctcgtttggc gcctgggccg gtcactgctc attagggtca tagtcctctt
      601 tcggatatag tcacgaaggt gggcttgtgc cttcccatcc catgaccagc gtttcaatcc
      661 cgaactcatc gcctctcacc catcacacaa tccccgctgc caccgagatc tgtttgcgcg
      721 tcggcagtct cactctgagc gagcacacac cgccgagctg gagcggcaag gtgccggaaa
      781 gtcgccaacc ggcaagtggc tttcgacacg tgcgattcat cggatcatca cggaaggcct
      841 gttgttagct ggagtgaaaa agccgggcat cgtggtccat agtctgcgtc ctcgtcccaa
      901 ccgttgcact tggaacttga aaccgccatg ctaatctaat gcaggtccag aagatgatgc
      961 gccatcagca ctatgcgacc accgagatcc atgtcgagga cgtgcagaag ctactggagg
     1021 gggcagaaga ggcggccacg cagatctaac tccgcaagat tttgaatcgg ctgcgagaga
     1081 tatgagagaa caaggcagca ggactcagac atacacaagc cgcaactatt tctcttcgtg
     1141 agtgctcatc ccagtcttcc ctctgagccg catcggacag agaagactgt ttcacattgc
     1201 gacatattct cttcctaccc aacgtatctt tttcgattca gtgtgaatca aattggatac
     1261 ggttggaacc tctgctgggt cagtgcacca gacatcacgc tgaaattatt ccgaatggtt
     1321 catgaatgct gcagaaaaat actgtgcatc aaacttgctt tccgatctgg taggcctgac
     1381 ggcaaattat tatgcttgac tgaatcacgg atacccataa gcataagcct catgatcatc
     1441 cgggacttga caactatatc gagatatttt gtcgatttcg actacatcag ccccaatgac
     1501 ttcgaacgat tggcgaaagt ggcataactg agtggctgtt tctatttgac cacaacacga
     1561 ttttcagcgt acgcaagtcg acttagctgc agtaagagaa tttgagggat gcaacatgac
     1621 tttccacggc agaattgagt cggccgatgg aggtgtcccc aagaacggct gggatgcgta
     1681 tcggctcgtt ttcacgtctt ccgcggcagt cgacggtgta ccgaggtctc tcgatcgctc
     1741 gatcacaatt cgcggtgacg gcagttattc tgccgatcct ggtgacccca tccccgcgga
     1801 tgcatccctc tccgtcgtat ctcccgaggg gcaaatactg gcgcgccgac aattctcatc
     1861 gctggttgaa gatggcaccc cttatctcaa acccttcccg gtacgtcccc gcgacctgga
     1921 cctcgtcatc gatcccgttg acgagccggt ggatcagcgg acccgtgtct ccggccgggt
     1981 ctttgttgcc gcaggcgcat ccagcgatca ctccgccgcg gcggaactcg cggtcaggat
     2041 tttcggtgcc tcgggcgacg gcgcccgctt cgcccccatt tttacaggcg tcactgatcg
     2101 gagcggatac ttcgccggtt tcgtgcaacg gcagcgcctc acggccgcat tcggcgaaac
     2161 cggagggggc gccgctcttg tccgccacac gatccacatc acgcctggga actggctccc
     2221 ggagcccgtg ctcttgttta tcgatgcgcc tgggaagacc agcgatgaag agaactgcgg
     2281 atgcacggcg cctccgcctc caactccggg ttctgcggag ctcgcggtgc acggtgaagc
     2341 attctcgacc gatctcggcg cgggctgtca ggattttacg gttcctaacc gcacgctgga
     2401 agagttcgac ttctttaaga tcgttcgcac gacggatccg gctatcaggg gcctcaccct
     2461 tcccgatacc gtgcgcaatg agatcgatct tccgcagata gcctcgctgg cattcgatcg
     2521 cggtacactg gcgcaggcag tcggagcgtc ggccatcgcg gtgcagaccg ccccaaagcc
     2581 gcccaccccg gagctaccgg tatcatctga cctccgtcga aacgcgactc catccgtcga
     2641 gggagcccgc acgatggctc gccttgcaac agtcgtcgcg aacaccgaag cggcggcagc
     2701 tgcgacgatg gccgacaccg gtatcgccga ttacgtcgac gtcagcccgc ttcagtcgtc
     2761 aggcgtgcgc cgagccgccg agtggggcaa ttccgtcgac gaaagggcga tgcaggccgc
     2821 cttacgtgaa gcggtggcaa agatcccgcc ggcggcaata aaggctgcac tcgaggaccc
     2881 tgatggcttc accccagaag ggttgatgac gctgcagcgc agtgtctccg ccaaagctct
     2941 gagagattac ctgaactggc gcatcaagcc ggtggcaaac cgaggcgagc tgaacgagat
     3001 caatctcgtc gactgggact tcacgcccga gttctatcag gcaacttcca tcgcccatgg
     3061 acacattctc catttcaaac aagaatggcg cgccgacggc tactcactcg gcgagttagt
     3121 gcgtagcatt ccgctcgctc caggtcagaa gaaacagatc gcggtgctcg agtgggatcg
     3181 cgcggatacc gcggcgcgca ccgaagccac tgacgtctcc gaagccgtca gtgcaactct
     3241 tagccgcgac cgcgacatcg acgagatcgc caacgttgcc ttcagcgaat cgctacgcgg
     3301 cggctcccgc gcgaccacgt ctgccggcgg tggcggattc ggcctggcga ttggacctct
     3361 cgtgatcggc ggaggaggag gggccgccaa agccagttcc aatgcctgga gcgacaactc
     3421 gcggagcctt tccggccaga cgctgaacca gcttcgggat cgcgtttcac agggcgcctc
     3481 tgcggttcgc aatcagcgtt ctaccgtggt tgaaaccgtc ggccaaagcg agcgtgtgac
     3541 cgccacgacg gaagtgattg caaactacaa tcgctgccac gctctgacca tccagtactt
     3601 tgaggtgctt cgtcatttcg cggtgcacga aacactggcc agcgtccagg aatgcctgtt
     3661 cgtaccgctg gaaatgacca ccttcgatga cgccaaagtg cttcgctggc atgacatgct
     3721 cttgaatgcc tgccgtgatc gtcgtctgac cccgggcttc gaagccattc gccgcttgaa
     3781 ttccaccgag ccgctgccgc ccaatcgccg ttacgccgac gacccgatcg aagagatgac
     3841 cggcaacctg cgagtgcgca tctcgatcgc gcgtcccaag gatccgaagg acgcgaccgt
     3901 tgccgtgcag gatacggagt ggcgcttctt cggtttcgtg ctccgtgtca accctgaggt
     3961 tctgtacgaa gtctaccggc gcgatcaggt caaacgcgac aagatcttcc gcaccgagat
     4021 cgctccgcag atcgcccagg catttctgga gagcctgcgc gtcatgcttg tcgaccgtga
     4081 tggccgcgag cacgacgccg gtttcgacat cacggtcgca tcccgctacg ccgagaacgg
     4141 tgtgatggag gtcgagctca acaacaccgg ccgcggacca cggctgacgc gcgcgcagat
     4201 tgcgggtgtc tccatccaga ccgacttcga actgccggag ttttcgaagg tcatcgtcga
     4261 atcggcttca gcctcttatg ccacggagcg cttcagtggc tctctgttta ccggatccgt
     4321 caacgacgat gtcgtgaagg gcgatccggc attcctctct gcgagccagc taagccgcgc
     4381 cgaagaaaga aatcagctcg cagagatgcg ccgcctgcgc cgccgtctcc tgcgccacct
     4441 caatgacaac atcgagtact accatcgcgc gatctggttg ggcatggatg cgaatcgccg
     4501 ctacatgctg ctcgacggct ttgaagcacc ccgatcggac ggtcgctccg tcgcctcggt
     4561 cgtcgagaat cgtctggtag ggatcgtagg caactgcctc gtcatgccgg tggctcccgg
     4621 attccagctc gatcccgtac tggccgaagc gctcgccgaa gaagaggatc cgcaaaaggc
     4681 cctcgagcgt ctctacaagg tcgaggcggc gccgccgcgc cgcctcagcg tcccgacgaa
     4741 aggagtattc gctgaggcta tgcccggcaa gtgcaacagc tgcgagatca tcgaagagga
     4801 tcgcttctgg cgctggaagg attttccgct gccggattcg ccctccgcga tcagcgctct
     4861 gtccacggcc agccgctttg ctgcgccggg aagcctccgt cccaccgctc ttccggacgc
     4921 catcgtcaag ttccaggctc tgccctcctt gcccgacccc accggcatgg cggccgcact
     4981 cagcctgctg ggtcaggacg tgttcaaaga cctcaccggc ttgacgaaca accagaagaa
     5041 cgccatggca gcgctgacca ccgcgatggg aaccagcgag gcgttcgcgg gcgaagcgtt
     5101 caaactcgcc atggccaaag atgccgcgcg caatctcgat cgcacgctgg agcagatcaa
     5161 ggccgccaag aaggcagact tgctgaccgc ggaggaagcg agcaaggcca cgcgcgacgc
     5221 catcttgcgg tcgattgcgg agacaccggc atcatccaaa gacgttgtga ccactccgga
     5281 agtggaagag gctatcaaaa aggccgcgga gacgccaggc ggctcggcga agatcacacg
     5341 cgaaaaagga gaaaagaccg aagaggtttc attcgccgtc gatgaatcgg ggctgcagat
     5401 cggcagcgcc accggtcacg tgcgggaata cccggtggaa atctgggtcg aagtgccgct
     5461 ggtccacgat gtcgtagatg acaccacttt ggactattcc gattccgacc gagtggacac
     5521 ccttgagccg gcgcgacaga aagaagccgt tcccgctaaa ctcgccggaa ccgacattct
     5581 cgtcgaccgg atcggcagcg ccatcgatag cgacttgatg cgagaaggtc cgagcggcag
     5641 gctgcagatc cgggtcaacg ccatgatcgg atatccggcc gacccggcgg acaacaagaa
     5701 gatccttcag ccgcaaggca aaacgaagta tggtttcgtc gcaatgatcc acggcaactc
     5761 caccgcgtgg aagctcgaag atggcccggt gaccgatgcg ccgccgatca ccggcagctc
     5821 gctggacgtg aaaaccgtcc ccgtcacaga gaggggagat taccggggat acacgtatct
     5881 tcagaagcac ctcgccgagc tgccgcgtcc gatcgtttcg atcagcttct cccatggatt
     5941 accgaacatg ctcgaccact tcgtggagac gcgatcccga atgctgttag ccgccatcgg
     6001 tcaggtcgtc gcggacgccg cacagccggg gtcgcttctt ggcgacaccg tggatttcga
     6061 tgccaccggg ctgatggggc attcccgcgg cggcgaggct gtcgtgcgag cgttcgagca
     6121 gtcaaagggc atggcaggtt tcacggtcac cgcgatctgc tcgcttgccc ccacggattt
     6181 cctcggtgcc agcaacaact tcaagctttc gctgccgccc cgggaaggcg tcaattacct
     6241 cgtcttctat ggcggattgg acggcgacgt cgtggggtgg cgggaaacga cgaccgcgga
     6301 tcgtggcgga aatggcttcc gtctctacga ccgcaccgag tgcgataaag cgatggtctt
     6361 catcccacgc gccacacacg accgtttcaa cacagtgtgg gccgccaacg aagaggtaga
     6421 gtggccgacc tcgaaacacg gcgtcactct gccgggcgtg cctgatcgga ctttgagcaa
     6481 atccacccat gaggcactcg ctaaagaatt cattggcggc ttcttcgact tgatcttgaa
     6541 tggcaattca ggcgagcagg cgcgcttccg gaacgaagtg aagagtccca ccggcgccgc
     6601 tatttcccat caatggcagt ttgctacggc aagccagcca ctcgacacct tcactgggac
     6661 ggcgcctgat ttcggcaccc gaacgcttcc agccggcgcc accattgagc gcttcgctcc
     6721 actgacgcca gttgccagcc gcgatgagaa gatcccgcac gacgacaacg cctgcattct
     6781 ggccacttct tcagcgggca cgccggcgat tgtgcacgag ttcacagggg gtccaccaag
     6841 cagcttcgat accatacgct tccgcctcgg gcgtctctat ccatcattgc agaaaagcga
     6901 cgtcgacacc gcagttacac tcagcccggc aagcgtcgat gttgcgaagt tacgagcgct
     6961 cctgaaaaca aaacaggagg atgagatcaa cctggtgaag ccgcctgagt tcgacgtcat
     7021 tctcgaagac accgataata agacggctcg cattgcgcat gcctccctcc tgttgggtca
     7081 acagaacggc tgggcacggc cggaagccaa agttccggtc gaggtcgtaa agttgccaag
     7141 cggtgacatc ttcgctgtcg ccaaggattg cacgctgatg tcgctgcaga ctctgcaaat
     7201 caaaaccgtg cagctggtcg gtatcgatcc ctcgaagctt cgaaagatca gctttgagtt
     7261 taaaacgccg gctacggccc aggaagtctg gctggacagt atcgacttcg tccttacgta
     7321 ggagcaagcc atgaacttca agagtctcac ggtgcttgca gtccctgagg aagtacagac
     7381 gaccaccggc tttgtggcgc gcgtcttcgc cgaaatagag agtgtccccg cgaccgggct
     7441 tcccttgacc gcggaaattg gcggtcaaag gatggaagcg ctcggggtcg tgcagggact
     7501 cgaacctctg ctcagcggct ttgtcagagt cgtgccgcag cccggtgacg agctggtcat
     7561 caaaatcggg gaggtcacca tccccaccgg cctgacagtc agcgatccgg caatcgcgtg
     7621 aggagaaaga aggcaatgaa gctcaatgga cagctcaccg gcgcacgttc gggctatgcg
     7681 ggacatcagg tcgaaatctt tatccccgcc cttatctctg ccgagaccgg agctcgcatc
     7741 ctggcaccaa cgttcgtgcg cgtgccggta tcggatgatg gcaagttcag cttcgatgtc
     7801 aaatccggcc tcacgctcag cagcccgatg gaggtcgcgg ttcgcgctcc ctccggcgag
     7861 cgcctcgcct tcctgccgca gccaacgctt tccgacttca ccatcaaggt acttccgcat
     7921 accgcagtgc cggtggcacc ggaaacgtcc cccaccgatc ctgcgcttgg acctctcaag
     7981 acgcgcatca acctccatga caaggcggaa caggatcgcg ccgcaaacca gttggtgtcg
     8041 gtctggggca ttcaccccgt cgccggagga accgagcccg ttcagaatgt gctgggcgcc
     8101 tcgtatgccg acccccgagg ggttgcagtt gtcgagatcc cgcgcgcggc gtggcggtcc
     8161 ctggaaatcg aggtgccggg acatcgcggg ccgaacaagc gattcgcgct gaagcagacc
     8221 cccgatggac tgcccgtcga atccaccgat atcctggttg accttacgcc acttccgtcg
     8281 tcgactgaag aagcccagtg cgactgcaaa gaatcaacgc tgccgagaac gcctggtcat
     8341 gaagaacttg tcggctccgg ggtgttcgcg gccgatccgg ttgcgggcgg ttgtgtcaac
     8401 cttgccgttc cgaatcgcac gatcgaggag tacaacttcc agtccctcgt tcgcacgacc
     8461 gatccattcc tgttcacgtc gaagccccca cgcccgcgtc cgctgcctcc gttatcgccg
     8521 acagcgttga ccttggcggc ggaactcgcc tatggcgtga acgcactgga cacgcagtta
     8581 gggcaggctg cggccggcgt cacagggacg cagccgagca cgcgcggcac agccgttcta
     8641 cgcaatggcg aggcggcgct cgatgcatta cggctggaaa gtcacaccta tcgctcaacc
     8701 ggtctcgccg ccgttcgcgc gtggcgtgaa accgccgcga aggacagagt cgtgaacgcc
     8761 atcatggagc gggcttcgtc gctgtcggag gatacgctcc gccatgccct ggccgacccg
     8821 gacggcttta cgcccgtcgc cctcatgacc gccgaacggc atcgcgcgtt cgagcaactc
     8881 ggacaagcgt cggagacgac tcccgcaagc ggcagaaccc cgatcgccgc cggcaacatg
     8941 ccgcagtggg atgctcagcc gaagacgtac caggctgcca ccatcgcgca cggacacctg
     9001 cttgagtggc ggcaggtgtg gcgcgccgac ggctactcgc tcggcgatct gctctactcg
     9061 ctgccgcttg caccggggca aaagcgccag atcgtggtcg tcgactggga tcgccgggag
     9121 accggcatgc gaacggaatc gcgcaccgta gccgaatctt tcagcgcgga cctgagccgg
     9181 gatcgagacg ttgccgagat cgtgaactcc accatcaccg aaagcattcg cgccggttcc
     9241 agtacgcgca cctggggcgg cggaggcggt ctggggctgg gcattccgtt ttcgggaggt
     9301 ttctttgggc tcggagtggc cggcgggggc ggcgggtccg actctcaagc ctggcagaat
     9361 tccgcccgct cgttaagcgc atcggcggga caagacatct ccgaccgcac gcagcaggca
     9421 gccacagccg tgcgtagcca gcgcgcgacc gtcgtcaccg gccgccagca gcacgaatct
     9481 gtatcggtca ccgcggaggt ggtcgccaac tacaatcact gccattccat gaccgtcgag
     9541 tacttcgaag tcctgaagca ttaccgcgtg gatctcgagc ttgcgtccgc tcagcagtgc
     9601 ctgttcatcc cgctccagat gacgcgcttt gacgagatga agtcgctacg ctggcgcgat
     9661 gttctctcgc aacacctccg cgaccgcacg ctggcaggcg ggttcgatgc catcgagcgc
     9721 attcttaccg gatatgtaga cagcgatttt ccgcccgccc ggtacgccga cgaagcgatg
     9781 accgagctgt ggggcgaact ccgcgtcgaa ctcgatatcc gccggccgcg ggagccgcgc
     9841 gagaacgaag acatcaacga ctacctgaat aacaactgga atacatggaa tatcctgttc
     9901 ggccccggcg ccgccgcgcg agcctatgac cagagcgtcc gagagcgcgc tttggccgac
     9961 aagatcttcg ccgatgaact ggcgcctcgc attgccaggg cgttcgcgga cacgctgcag
    10021 ttcgagctgt gcgtcgatgt caacggaacc ccggtgtacg ttccggtcac tgccgacttc
    10081 accatggtct cggactatcg tgcgggccgc gagcatctca ttgccttccg ctgcgtcctc
    10141 ccatcaaatc agcaggtcgc gcgcgctcgc atcaaggccg ttcgattttc tacgacactc
    10201 ggcgagatca ctccgggctc ccgcagcatc ctccgctacg tgcatgtcag ttatcgcaac
    10261 cgctaccgca gctttgcgtt gctgtctccc cggagagtac gtgacgagat caaagccgga
    10321 gacgcggtat tcctcgccac gtacgctttg gcgaaatggg aggagctgaa cccgcggttg
    10381 gaagacttgc gcctgcgcga tcgtcttctc aagcatctca acgagcatgt tgagcactat
    10441 catcatgtga tctggtggct catggatcca ggcagacgtt tcatgttgct cgacgggttc
    10501 atcgcccccg attccgaagg ccgttcagtc gcctccgtga ccgaaaaccg gctcatcagc
    10561 atcataggca actcgcttgt gctgcccgtt gcacctggtt atgtactgga tcccgtcgta
    10621 aagaatgcgg aggatccgga caggcccatc gacctactgg aggcctaccg tccggcgatt
    10681 ccctttgcgc caaagcgaat cagcgtgccg acacgcggtg tccacgccga agccattctg
    10741 ggcgagtgca acagttgtgc gatcctcgac aacaagcgtt tctgggactg ggcgtccgag
    10801 ccgattgaag aggagccgac gccgattcag gaaacctcca cggagacgcg ccgccagact
    10861 ccgcccgaca cggaaccgac gcccttcacg gcgccgatcg tcggaatcca gaacgtgccc
    10921 gaagcgccgg cgcccactgc gctcaccgat ttcaccaagc tactcgggca gaaggatctg
    10981 ttcaaggacg tcaccggcct tgagttgaac caggccaatg ctctgggggc gttccagcga
    11041 gctatgcaaa cggcgaactt cttcggcaag atggcggcgg ctggagcgaa agcctcattc
    11101 gcgaatcgcc aaagcgaaaa cgtcatgaag aaggtcaccg aagcggtgaa gaacggcgac
    11161 atgagcaagg aagacgccaa aacggtcatc gataagctga tcggttcgat gaacggaacg
    11221 ggtggcgaaa gcgacaagcc gctcaccggc gaaaaggccg tcaaggatgc gttgaacaag
    11281 gtcgtcagcg gttccggaaa agtgaatgtc tccagcgaaa gcggcgacgg caaacaaaca
    11341 gtcgaggcga agttcgcaga caaaaacgga agcgcacaat ccaccgatgt gcagattgac
    11401 ggcgtgcccg ctgtcgacca ggtcaagtcg cagggttgct gggcggcagc cctcacgatg
    11461 ctgatctcgt ttcagcggaa ggcaaccatg ccgattgaga cggtcctcgc cgaaggcgga
    11521 ccggagtatg tcgagaagta tcaaagcaac ggagggatca aaccttcgga aatcgcagcg
    11581 ctcaaggaca cgttcaagct gcgcgatgcc agcagcggag ccatcacgct ggcgtcgctt
    11641 tcgagccgcc tctccgagcg aggaccgtta tggatcgccg tcgatgacga tccctcgcaa
    11701 cagttctccg cacatgcgcg ggtgatcacc ggactcaagg gaaacatggt ccggtttatc
    11761 gatccggatg gtggagtgga aggggaagag gaattcgtgg tgctcgttcg acaactgaac
    11821 gaattgagca aaggcgtaag gagtgcgttt ggcggatacg ccccgctgat tctctctctc
    11881 tagagcagtt tgcatttaaa cagacacata ccctgccgat ttgaagtagt tggcgcattc
    11941 ctgcggagag aaggcgtcga ggagtgttcc gatttcgttc caaagcgcct cgacggtgcg
    12001 gtgagcggct tttttgagca aggctttgag cttggagaag accatttcga tggggttgaa
    12061 gtccggggag tacggcggca gatagcggat ggtggcgccg accccttcga tggcttcgcg
    12121 cacgtcgtcg ttcttatggc tgccgagatt gtcggcaatg acgatatctc ctacgtgcag
    12181 tgccggacag aggaactcgc gcacgtagac gaggaacgct tctttgtcca tggggccgtc
    12241 gagcaccatc ggcgcgatca cggcccccac gcgtagcccg gcgatgaagg tggtggtgtt
    12301 ccagtgtccg cccggtacgc tgtgaaatat gatcttcttg gcaagctggc ggaagcgacc
    12361 cagctcactc ggcggaccat tgccaccatc ctgcgcggta tcaacctcgc cgtactgggt
    12421 caatacaaaa ccaaccctga agatttcatt ttgaaggcag gcacactcat taatgaacaa
    12481 aaagccacgg tgatcatcga gcatctgacg tacaatccac tggaagagac ccacaccatc
    12541 gacatcttcg cacagcaaaa gaaggaagat ctgagcaagg ggttcaaggc caaccgccac
    12601 atttacgatt acgttcttac cgattccagc aatgagcgca cgtttgtcgg cgaactcgat
    12661 gccagttctg aagtcgtggt gtatgccaag ttgcccaaag ccttccacat tccaacccca
    12721 gtcgggaact ataatccaga ctgggctatt gcctttgagg ccggacaagt gaagcacgtc
    12781 tacttcgtgg cggagaccaa aggctcgatg tcgtcaatgg acctgcggaa gatcgaggaa
    12841 actaaaatcg aatgcgcccg caaattcttt gccaaaatca cgtcagacca ggcgaagtat
    12901 gacgtcgtga acagctacgg aaagctgatg gaactggtga agtaggcgag gacctctgtt
    12961 cgctctaatc ggcctgctgc cggtttagtt gacaccatcc tacgctcatt tagcctgccg
    13021 ttcgaactcc atcggactga gataccccaa cgtggagtgc cgccttcgac gcccgccacc
    13081 tcacgaaaca aatcaggcgc atggaacaat gcgttcaatc cgatccggct cacgccatcg
    13141 gaacggccaa agagctgatc gaaacctgtt gcaagaccat ccttgcacag cgcgggaaat
    13201 cgcttcctgg gaaacctgat atcccggaac ttaccaaagc caccctcaag gaactgaagc
    13261 tcgttcccga cgatgtagaa gattcagcgc gcggcagcga cattattaaa cgcctccttc
    13321 aaaatctcgg aaccatcgga aacaacctcg ctgagctacg cggcctttac ggcaccgggc
    13381 atggcaagca tgcccatacg ggaacactga caccacgcca tgccagactt gccgtcggcg
    13441 cagcatccac tctcgctaca ttcctcttcg atactcacag agagagagca agttgactgt
    13501 cgctaagaac atgcagcata cgaatcagta tctgtacgga cgtacagaga gcctcagctc
    13561 cccgcgcttc acccaaggcc ggtaccgctg atgtcctgga gccgcacttt cacgttaatc
    13621 atgccgattg acattgtagg ctcttccgtc ttcctgggca tgaccgttct tagcagacgt
    13681 catacaagcg attgcgtcgt ttttcttccg ccgcctcgcg accgccaatc acaaaataga
    13741 cataaggaac acagagagtg gcaatagtga ccagaataac cattgtcgcc gacggaatca
    13801 tgacttcacg catactgatc acccagtcct gcaccccgac taccatctga tcggacatca
    13861 ctccccggag tatcggccag agcagcttga ccccctcctg tacatgttcc ggcgtcaaga
    13921 gctgatacat aaaattaaaa aagaccgaca ggagaatgac cttgatgatg ttggccacga
    13981 tcatcgactg atatatgtcc tgaatggccc gtttgcagac ctttccgatg taacaggagc
    14041 cgaggttatg tccaatcatg accgcatacc ccacgccgta ggtgacccca atgagctgcg
    14101 cgaatacctg atcgaagagg ctcggatctg gatctccgaa gatcggcatc aagctcccta
    14161 agacgcccag tcccatcggt acccctagca tgttgatcag cccatcgacc acgacgctat
    14221 gcacgcccac ctcggtatag gcaaagctgc gatcgagcag aatgagatgt tccggaagcg
    14281 gcgtaccgag cttctcgacc accgccaact cctgaatttc gctgatcgcc gcaatcagac
    14341 ttcgcggacg gcttcggcct gaagccccct ccgcaaagat cggtcctcgc cgcgccgctg
    14401 attgctccat tgttacatgc tccccgagcc gatcaggttg atcagtggtc gaaaatatgc
    14461 cagcagaatc gtcagcatga aaaataagaa ccccagcgca atgcgctgtt tgtaggcccc
    14521 aaaccagaac gtcgctgcca gagtggcgga ggccaggatc cggagcggga caccggccag
    14581 cagcgtcgta ttcacccacg atgacacggc aataaatgtt tccgggtcca tatacctgca
    14641 ctcctatcct cgaccgagtt ggaattcggt cactaactgc ataaaggcca ccttgcgaga
    14701 ctcgagttcc gattcggcga tccgaaatgc ctccaatttg accggcacga aatagccccg
    14761 ctgtttgatc cccgctttct tcgtgaaaat gtcgaacggc cggccgtaga aatgctcccc
    14821 aatctccatg gccaagagat ctgcagcacc caggattttc accagtctcc ggagcgtatc
    14881 ctctcgatcg gtcgatcgaa tgagtcccat ttcgacgatc ttcttctgcc gtgccagctc
    14941 tttggccgcc tccagaaggg cgtcgggcgt cacatagacg acccctttga gggaaaacgc
    15001 cgcccactta tttcctttga gtggacgatt gatccgaggc gccacaatcg ccaagacctc
    15061 tgatcccgcg caccagctgt ctaacggctt ctcctgcatc tcctgcgtac tgaggatcat
    15121 ctccttcacg cgagcctgcc catcggcttg ccgaagaatc acgcccagcc gatccttccc
    15181 gatccgatga tggcccttga tggcatccaa cacttcactg aaccatggga tggacgtccc
    15241 agcaaagcac tcttggagct tgagagcact cacgaccgga tgatccccca tcgcatgggc
    15301 ggccgtggca cgaaaacgtg gaatcttgcc cagatcatgc cccagtgctg ccaccagtac
    15361 cttcggaatc atgagatccc cgtcccggta ggtttgcttg agcaccgcca acgctaactc
    15421 cgtgacccga taggtatggt ctcgcaacgt gactttcgcg agcgtatcgc gaatggaata
    15481 gaggtccgta cattcctcat cacgttcgac accggccgtc acaatcgatg gacaatcccc
    15541 atattcctcc agaattttca ccaccgtcag cagtggctcc agtgcgcgct ggctctcaat
    15601 caccggttga tagggcatga tgaaatcccg aatgagtgcg gacgaccggc cagaggacac
    15661 cgggcgtggt gaggtggcgg gatcgggtgt ggctgcctgt gggacttcct caggaggagt
    15721 cgtcagctgg gcggaggatg ggccaaccac gagagcgggt gcactgggag atgccaggga
    15781 tgcaggagcg atggtctcct caagctcagc cgcggcatgc tccactggac tgaccgtcgg
    15841 ctcagcttcc tcgaccaact cccctgtttg ctgaatgaag tccaaattca gcggcagcca
    15901 tacgtctttg gccaggtctt ccagggtgat ctcaatctgg ctcggtacca cccgctgagt
    15961 tgtttctcca gcgtcgattc ttgcagagcc gccgcgccac gctccccaca atctcccgag
    16021 catgtcacct ccagggttcc ggtaacggca tcacgctcac atgcgccgct ttagccctga
    16081 cacgcacaac ccgccccatc aagctcacat ctatcaatac atcctcgcct tccaaggctc
    16141 gcacgatccc cgtcatctgc gcatagggcc cagaactgac ctgcaccaga tcggaaacct
    16201 tagccggaag tggctcattg ctgaggtcct cgaagacgtc ggtatctact agaggcagcg
    16261 ctctcaccac tccaggcagc cccttgatca cgggaacggc cgtcggatca cactgagcga
    16321 tcacgtagcc tttcgttgga cgttccacgc tcgtgcaccc cagctcccgg agctgcaaga
    16381 ccacgtgttt ttcacggcct ggcgtcacat caaacgccca ccgtgcgcgc gacacattcc
    16441 cagtggcaca ctgctctgct tctcggttgc tcacagactc tcctttcgaa agccgtaccg
    16501 gcccagccag cgtactcatc gcagcaaccc gcgggctcgt ctgatgcctg cgagccgctc
    16561 aagtgcccgg tacaccttcc agctgtagcc cgcgatgttg cgactcccgt tgtatcggcc
    16621 tacggctgtc cacgtttctt ggtactcgtt gatcttctgc cgcaagacaa acgccgccca
    16681 ttggatattg atgcagggat cgagaaaatg gaccggatcg attttgtagt gccgcatatg
    16741 cacgctgttg atctgcgcga ttccgacatc aaaattcgcc cccgtcttcc acagttccgc
    16801 caccaatgcc ttggcttgct ttaaggatag tggtcctcgt cgatccccct tcccatcccg
    16861 gttcacattc actgcatgcg gatccccttc gctctccacc agaatgatcg cttccaatat
    16921 ttgtacggtc accgcgtagc ggttcgccgc cgccacgacg caactgtggt tcacatacca
    16981 aaaccggtcc gggggcatct ccgccccgac actgtccggc aagacaacga gactcaccac
    17041 cgcaagacac agccacgttg aacggagact ttgtggtctg gaggatgtca ccctgacgcc
    17101 cacgatactc ccgccctaac aagtgcccgt gtaggtacat attttcgtcg ccatttcctt
    17161 cgtgatcacg gccgggtcaa tattcgctga cacatttttg taccattcgg tgaaatccat
    17221 cgcctggaaa ttcaacccgc tgaactgatc taccgtaaat ccgtcacact ggggcgcatc
    17281 cgctgatccc caggtcatgc ccaactgcgc acggccttgc tcttgaacca ttcgcccgag
    17341 ctcggatttc atagtgcagt agacctcttc tttgcggagg cacacaccaa acggcccctt
    17401 atccacgcaa tgtgttccaa tcaaacgggc cttccaatcc tgtttgtcac gcgccagcgc
    17461 gatctcctca tccgagcatt tccctatcaa tttgtcgggg tccgaatcac agcaattgat
    17521 cagggagcgc ccgatcgtgc tgaaatcaca ggtactcctt ttgcccgtga agatccgcag
    17581 ccggtccgga tcccaatcct cttcgacatt cttcgtcatc gataaccagg ttgccgccaa
    17641 cgccagattc tgatttgggg gagtgttggt atccacgcaa aacggcactg aactattctg
    17701 gctgccgcat gtgatggaat ccggcggtag ggtctccgcc gcagattgag ccgtcgtcac
    17761 tgcaccgctc gtccgtaagt cccatccatc acggtccacc ctgctctgcc gtatttgggt
    17821 gtacctctca tcgctaccgg cggccgtgcc gccagccgca ctcaaacttc caggatcatc
    17881 gtaatacttc tcttcagcgg ccccggaaca tcctgccgtg acacagcccg gcgttcctga
    17941 gtaccgcggt actcttgtca tgttcggcgc caaaatcccc ccaccaatca agcccgagga
    18001 cttttgcccc tccacctcgc actttgcttt gaggtaggca tcatcaatcc cgctacaatc
    18061 ggcacgggcc tctctcgcac cacacgccag acagaccgca aggaaaaccc ccgtgagcgt
    18121 gcgtttcatc gctaacagcc tcctctatac ccgcgtaccg aaccgagccg tatccaacga
    18181 tggcaccggc atctcggcca tccctgcggc ttctccctgg aaattcccgt ccgccggatg
    18241 ttgtggaggc tcgccactcg cgacgaaggg ggccgattca cccgcaaaga tcgtccccat
    18301 cgcgtgacga tctgtaaact ggatcggcgt tccgcgcatg acgatttgcg ccatccccca
    18361 cccccagtac cgtgcggtca cctgatggat ctgaatgtcg aatccaataa tcaggtcggc
    18421 ccccagggag gcagcgcttt gcgcaagccg atccaccatg ctctgagtcg catggacgat
    18481 cgggctgtcg tatgtcttcg cgcgcccgcc ccagaaatcc gtgaaacgga ccacggcatc
    18541 ccgccaggac gcgatcccga cagtctcgat ctgaatcaac gcctgaaagt accgataggc
    18601 atgcccaggg atgacatcta ccgtgagaat tttcattcct gcctatcgct tctcggtatc
    18661 aatcggtgac acctcagcct tcttcggggc atccccctcg gtgctgatcg tcagtgtgtt
    18721 catcggcgcg gccatcacct cgccatcgac cgacggagga agggggcgtc caggtgaggc
    18781 ttttcctact gaaccggcag tcggtggagt agacacaggt tccgcgactt gcccgttccc
    18841 cccacggaag ccatccagaa aacgccgcaa ccaccctttg tccgcatcgg gcttggaacc
    18901 agccggttcg aacttggcct cgaccacctt cggagaaaca accatcggaa catccggttt
    18961 agccggggcc gtggttggcg ccaccgggct cggagcggcc gccaggctcg tcccacctgg
    19021 ttcactaccg gagcccactg cccctgcacc ggcactcccc tccatattcg gactgcccgc
    19081 cttggcgaca gggtccgctt tctgagcacc tcccgacttt ccctcaccca cctgaacacg
    19141 cgttttggag acctcgggag caccccctcg cgcctgcagg gacttcccta gtttccctat
    19201 cgacagttcg acacgttcga agcgattggc taaggcgacc gtcgtttcgg tgagggtttc
    19261 gatcttcccc gcaatatttt gcagacgcgc gtcgacaccg gacagttgtt gtgtctgctg
    19321 cgcaaactgc gcccgctggg cctcgtactg actctgggta tctttcacca cgcccttcac
    19381 ggtcaggatc tccccctgca gcgtttccgc ttgagccttc aaccccattt gctcttgcgc
    19441 gacatgccga tacacctggg acacgttatc gactcggtgg gacagctcgt catgttcggt
    19501 ctccatgact tccttcactg cgagttgatg ctggtacttg gtttccaagt cctgatattc
    19561 cccggcgctg acgcaaccgg acaacaccgc gagaccaacg accgccatgc gtggccacat
    19621 accgtgctcc tttcttacga cagagttgac tgactctctt tcccttcctg gagaaatgcc
    19681 ttcaccgcgc cccgcggttc atactccgtc accgtcacgc gggcgacatg accgatgaca
    19741 tcgtgcaggg ccactacgag ctcgtcgacg gcctgaaaat gtttcacata cggactgaga
    19801 tcttccgctc ggagacgaat actgttgagc gcgctatcga gtgcgcgaat caccggcacc
    19861 agtctcaccg tgtctcccac ccgtggtgta atgacccgcg catttcgcct cgccgccatc
    19921 acgctccgct ctttcaaatc gggatacagc tcctggggaa gcaactcgaa ctcgcccact
    19981 ccaagttctc gtaggagccc ctttgcccgt gccacccatt gctcgacacg agccaactca
    20041 ttgcgcactt gctcaacgga gagctgagac cctgctgccc gccgaagccg atggacaacc
    20101 ttgtcgaaca tgtacaacag ggacacacag gccatccccg cctttgtctg tggtaacagg
    20161 agaatcgaat tcggatcttc catccgcttc ctgttctctt ccgcccgatc gggccttcgt
    20221 ccgttcgtcg gagacgcact ccgaagagac gggccctcac gccgtaccat ccccggttgc
    20281 tgttctacgt ccaccccatg agccatgcct cacctcctcg attacgcccc tcccacctta
    20341 cccagatccc cttcgtccac gagtaccagt ccatatccag tcaatttgct agccaccgca
    20401 gcattcatcc ccttgcgacc aagaaactgc gcaatcgtgc ccggctcgac gatcactcgc
    20461 gcttggtgct cctcccgata cacatccact cgacgcaccg accgactcgg agcaggcgct
    20521 aacgcctcgc gaatgtaaga ttccaactcc gagtgatact tcacgagagc gatcgtgcgg
    20581 gaggtgtagg tacgaaactc gtacaacagg ggcttgcagg ccgcgatgac atcttctccc
    20641 gagcgatagg tgtcgacagc caccttcaca aacgacgcgg ctcgagacgt cgccacgcgc
    20701 ttgatatgca catggtcatc cgccaagacc ggagccagca ctaactccgc gatacggcgg
    20761 aaatagtgca tcgatcgctg cgagagcatc ggaaactcgt caccgacctt atagacagcc
    20821 cccatcaggg aatcgcggac cttgtactga cgatcggcat gctgatgcgg caggatggcg
    20881 taccgcccga tggaatccgc ccacagcaga tatgcgctgt gccccggatc catccgaatg
    20941 acccgagcca tcactaagtc tcccacctgc accattcgtt actcgcctgc gctatagttt
    21001 tttgacctgt gtatgcacct gccatgtgcc tgtagttcga gacccggtct cacaactatg
    21061 cccaacaggt attaccggaa cagtccccgt ccgcttcggc tatcgtccag agagaaggtc
    21121 cgcgccctcg tggcacttac atcgctgtca tcttgccgga cgatcagcgt gatcctgctc
    21181 ggattggtca ccggggtatc ctgtgccccc gtcttcacgc cgaccacgtt gcagacgttc
    21241 aaagaatcgc gcgacatcac ctcaacgacg gctgtagagc cgccacgagt ggcaccgtca
    21301 ctccacagcg atggcttggg atggacggtg ggagtcacgc agctcgtcgg ggagacgaac
    21361 cggatcagac gagagggggt ttacagctgt tctttttggg taatggattc ccgcctctgt
    21421 ggcactcacc atcaggagcg gagcggccga tcaggagacg gccattctag agtttgtgga
    21481 tattcagaag gcggaacgaa tcagatatga cgtgttgcac tttagccgtg actggacgga
    21541 agatgcgcgt atgtttaagg actatctcca aaccatcttc cacgcaaaat atcagtcctg
    21601 ctcttagcag tctcacttcg catcctgcac ctcaagcacg caatcaccat acacagtgat
    21661 taacaatgac cctggatagg tcctataggt ctttttgtct gcctgaagtt cagaggtttc
    21721 cccgacagtg ttcacgatcc taatattaac cattgctcgc ccatttctgc ttccccgaca
    21781 ctgttctaca acttcattta atacagccag agaactccca ctggaggagg gactaaattg
    21841 ctccgaccca acagcgatag ttgagcgctt gattggtact cctggaatct ccgagccaac
    21901 gaacgcccct aaccatccat cttttggcga ctccgctctc gcaccacctg actgactgac
    21961 aacgattgcg accatcaccg caactagcgc tatctgatac ggccgcatgt gacctccact
    22021 ctggacaaaa ccattcgcta cctacttctc tttactagtg cttggggccg atggctgtgt
    22081 tcgctcaacc ttcaacaacg actcttggcc tacctgccac ttaggcatgg tcccaaacgc
    22141 attcgacgcc tcttcaccca acatgccgat ggccgatgtg cgtaccgcat tataggcggc
    22201 attggcagcg aagttctgta gttcttcggg agacattgtt tgtgcgcggc cggtcttttc
    22261 caaatgctcc acagaactac gcgcaaagga tatagcatta tccatcacgc cggaatactc
    22321 tttccaccag gcacccatat tttgatgcga gtccaagaga ccacttgggc ccccaccgcc
    22381 accaggtcca tccggcaggg gtccaccgcc tgactgaaac ggtgtccccc cagccgctcc
    22441 gccaccaaac ttccccattc gtcccttgag gatcaaatat cgcgaagccc catccattcc
    22501 aatccgacca aattcgaggc cataggcaat cgcctcgtcc gtttgcgtgg ggttggtaat
    22561 atcggtgcct ttggccgcaa acgccatttg ggtaatcgtc aaattcgctc cactatcatg
    22621 atgaaactgc acgcgattga agtcatgaaa atcggctccc ttgttaccgg acgaactcag
    22681 cacattcccg ctgctgtcga gagtgtcgtt gaccgcaaat ccatgggctg catactcatg
    22741 cttcgatagc tccctggtac ttgcaatcac gctcgcgccg tcaggcgact gtgtatactc
    22801 caccacctgg aagtgttctt gcccttgcgc atcggtgcgg tattgcccaa cctttcctgt
    22861 ttgcacatcc gtgacttcgg acgcaatcaa ctggccagat tgaggactat agtagaacgt
    22921 cccttgtaac tctttcgtct gtccgggatg cattggatca ggcacggtga agacgcctga
    22981 cttgcgctca ttgttaaagt actgcgagtc caatccgcct aacaccgagt gatacaagac
    23041 ctcgcgttgg cctccatcac ttttcatcgg accaaggact tgcgtgtcta ccgctccatt
    23101 cttggcagcc acgaccttga gtgtctgatc gaaagagccg ctcatagcct cgctgtttgg
    23161 atcttccaaa gacctggcaa tcccccacac ctcattgcct tcaccgtcct tcatgaaggt
    23221 ggaataggtg ttgtcggtca cgttggtaat cgtcccgccc agtaacttac gctcgcccgt
    23281 ctcactgctt gttggaccgt agtagagatt ggcgtataca tccatatcgc gatgcccgtc
    23341 attgatccgg cgccaacctg actctttatc gatcgactgt acttcatcgt tgtaccctcg
    23401 tagggactgc tcgaattcca cggtcatctg gcgcgatcct ctcatctgat cgctatacac
    23461 acggacccca tccaactcaa ctgacgagtt tttgaaggct cgcccctggg cgtccacaga
    23521 cacgccctca aaggtgctta cggaatgtgt gccccctgga gccatcaggg tatcttggaa
    23581 cccatattgc cgaccagaca ccccagatcc tttttcgaca atgtctctgg cgtgagaccc
    23641 agaggtgcca ttaaacgcca caaggttgcc attatcaatc agccctgtta catcacgggc
    23701 cggtgctcca ggttgctgag catatccatg aaacatctcg atttgatggc cgtcctgtcc
    23761 catcgtattc aattgggtga actgacctga caaatgcgtg ccatccgtca gattacgttc
    23821 cccggaaata ctcttggtcc ctccctctcg cccatcaaag aagcttacct ggccgtcgta
    23881 tagcagcgca ttggcttgaa acgccggcga ccctggcttg ctattgtcga tcgccgagcc
    23941 gaagaaggct gtcggcactc caggttgatt ggcttcccca tggctcaatg ccgtgaacgt
    24001 gccgctcagg gtatacgagc cgaccgtctg ggtgtcgtga gcgacgccgc tcgtgcctga
    24061 ctcgaagtgt tccgtggctt ccaccaggaa ctgccgccct gatgcatcgg tgcctaatgt
    24121 ccccgtcacc gtgtgcgcgt gcccaccctc atcactggtc agactgtaac tggagtagat
    24181 gtctcgcgga tgggcagggt ctgagcgatc gactgaccca aaggccctga catatccctc
    24241 catcggccga gtcacgccat ccttccccaa gaccatctgt tgtgcttgcc cagtctccac
    24301 aaaataattg gagccgtcag gggagacgaa cgattccctg tggacggcgg cttccgtatc
    24361 cttgttgtca aatcctcgga tcgccccgcc ctcggtcgcc agaaaatgaa actgtccatc
    24421 ccgttcgacc aaccggccgg tatacgattg cttcgccccc atcgtgctct gaaacatcga
    24481 ctgcgtgatg gtgtctgccc gctctcctgg cccacgctcc cccatcattt ggacaaatcc
    24541 ggcatcggga cgtgtctcgc ccgcgacttc cagaaacagc gggacattcc cagatcgtga
    24601 gaaactctga cttccatcgt cggcaatcgt acgcgtctcg gacaagcctt tccgaacaga
    24661 ttgttccgta cgcgccacga gttctccggt gtcctgattt tcctgtacga gcacgctctt
    24721 tctggcgccg tcctcaccca tcgtatcgaa acgatgctcc accatcgacc cgaacaccga
    24781 ggtttgtgca tcgacctgct tcccggactg aggatcggtc atcttgtgac ggaatacccc
    24841 actcaggagg tgccccttcg aatccatggc atactgccct tctgggcctt tgtagttttg
    24901 aataccattc gcgccgatac tggtgatgcc accctgggag tcgtgaatct cactcgttcc
    24961 ggattgacta tcgacacgaa atccaccggg gccggccggc acggtcatct gcggctcgac
    25021 gtcatggccg atcatctccc cgaactgggc accgcgctgt tcgtgtcgat aggagggccc
    25081 ttcctccaaa ttggcaccga gcccacgaga gaactgcccg cccacttgcc cagcgacagt
    25141 ttccgccgtt cgcgatcccg ccaggatcga tcccaacacc tggctgccac cccacatcag
    25201 tccaaacccg atcgccggca cgagccacac ataatcggca gccgtggagt tcagcatggc
    25261 gacatgttcg ttgattcggt ccaacgtcgc atagttgacg gactgcccca ctccgtttga
    25321 actcaagatc gcgcccagcc ggctccgcgc ttcccagaac atgacgaaat tcagtactgc
    25381 atacagaggc gaccaggtct gcagccacat gaacagccgc cagtaggtgc ccatcagccg
    25441 ggacatgccg ggcgaaatgg cgagcaccag aaggaacggg gtcatgatgt acaccacccc
    25501 ttccacgatg gtccgtaata acggcacgac cttctgcgcg acataggact tcgagtagct
    25561 ctggaacttc agatccttct ggatcgcctc ctgctgcaac agcacggtgt ccgcagttgc
    25621 cgcgcgacgt ttggcatcgg cggattcggc gtttttccac gcatgaatga gcagcacatt
    25681 gttgatcata tctcgagcgg attgcccgac ggacaacaag agattcgctc gaatatcctc
    25741 ggtgagccct tcctcattgc cggtggccgg atcgagctgc aactgcgtcc gcaaatgccg
    25801 catcgcccct tcgtagtcag gctggccgct ggtgtatcct gggaggataa gcgaactata
    25861 gaaatccgga cagacccgcg gagccgcctc caatgtacca cccgtatacc cgagcgccgt
    25921 gagtgcattg ttcatggctc cgacatcatt gagcagattc ggagaatttc tcagcgtgcc
    25981 gaggttcttc tgtagcagtc caacctcccg aaacacgcac atcataaaat aagtatccgt
    26041 attgcgagcc agatccgagt cttgcagtct cgcattcagc accttctgca gatcgaataa
    26101 cgggcgattg aatgatgttt gtccctgggt atattggacg ggcatcggaa aggacgcttc
    26161 gacgagctgg gtggtgtaat accccacttg ccccgagatg tatgccggca acgcgatcgc
    26221 cagcgggaca ttgttgacga tttgatcggg aacatcgggg ttgacttggt cttctacgga
    26281 cacatccacc gtgattcgaa cgctgacaaa gagaatgaaa aaggccccaa cgaccagcgg
    26341 gaccaagaac cgccgattga acacaaaata gacgagtccg aacatcgcaa agaccaggag
    26401 cgcgagctcc atgagacttt cgaatcgtcc ggtggtcatc ataatccgga tgcccagcag
    26461 aatctgggtc aacacatcgc cattgccgaa ggtatagatc gtccacatgg tccgctatcc
    26521 tctcccctca catcgcaatc aggcttcctc cgacgactac cgcagcgatc gcatttcgcc
    26581 ctgcgtcttc gtcaactgca tgagctggtt gatgttattc accatgcgtt gccgctttgc
    26641 gagactgtca ctggcccccc tcaaaacttg atgcagccgc ccctgaaaat cttgcagatt
    26701 gcccggccat tctggtttga ccttttggat atagctgacg ttccgggatg tttcggatgc
    26761 cgcccatttc acccattgat atcccatgtc tgcagccacc acttctgatg acaagcgcgc
    26821 aatctgattg ccgacatccc gtggccgccc ataatcagcg agcatggtca gaatcgggac
    26881 cggtgtcatg ttgatgattt gttgatccgc cgcactgatt gcagagcccg agtagaggtc
    26941 ttcgatgagg ttgctcatcg acgtatacac acgactttcg aatccttcga tatcggtcgc
    27001 atccgtaatc gtggggttca aacattcgga atcgcccccg caattaaata ttttgaccac
    27061 gtcccccaaa tggctgtcga ccagctgttc gaacgtcatc gtcggatcgc gggtttgaac
    27121 atcgccgtcg ttcccgacaa tgatggtacc tgtgaccgac atcaccatga caatgagatt
    27181 ctgatccagg ccggcctgcg tcagaacatc ccacaccaga ttacccgtca catcgtattc
    27241 cgtgcccttc agagctgagg ccgcatccgc attcgactgg ttcttgaccg catcgcggtc
    27301 ctcaaacacg tctccgatgg cgccccccgc ctccttcatc ttctgccagg catcgccgaa
    27361 ccgactcgca agcttctgtt tcgaggcttc gttcccggcc agcgccttac ccaattcaat
    27421 ccccgtttga cagggttgga tccgtcccgc ggcgttgagt gctctggcag cggcttcgat
    27481 cttattgagc acgtccgcgc agtcttcaca gatggctttc atcgccagct ggaacgcgta
    27541 ccccaagacg gccccggtcc cgatttgctg cagcaactgg acgtaccgat ccagcttgcc
    27601 gtaggaaaag gagcccagat acacatcgat gccttggcag cccacgctca atcgcggagg
    27661 ggcaatcgac cacagccgca tcgggtcctg ccacaaacga accgagaccc caccgccgct
    27721 gaagtaacct cgacgttgac tctcgtaggc accgggagga ctcgtgatcc cgacacccca
    27781 cgagctgaac atgttcgtga gggcatcatt catcgattgc gcctgcacca ggggaggcca
    27841 ggccagtacg gcccacatac acagtcccac cagaatccgt ttgcatcgca tcgtgtgtcc
    27901 tctatccatg agacggtcgc tcgcccaaca atcctcgatt ccgcaacagg accagcaatc
    27961 gctcccgtag atccgcattc gtcatcagcc cggccccgag tggctttaag aagttttcct
    28021 cgggaatcgc gagataaatc atgggcacca tatgcacgcc cacgttttcc gccatgccgt
    28081 tgttgatggc gggttgcggg tactccggca atcccacccc atccagcgac acaggtttca
    28141 ccgttagtcc gtaggtatcg gcgaagacct tcaatacctt ggactgttct tgacaaaacg
    28201 gacaggtaga ggtgaagaag aagaacagtc cgacccggtc ggctgtgcct gccagcagct
    28261 gctcttcggt gtttcgcttg atctcggatt cgacgctatg cccataggtg gacgtgggat
    28321 gttgcgacgc atagtcgagc ttggggtccg tgtacatggc gatctgccag gcatcggtga
    28381 agcgttgcga gcggtcatac gcttcctttt ggactttgat gtaactcgtc acattctcca
    28441 ccgagggatc gtccaacgcg cgttcctttt tggccgacac cagctcccgc aacaccgtca
    28501 ccgggagggc ggttaacttg actccatcca tcgcaagccc cttgaaccac gtccggtatt
    28561 tcgacggctc cagccaagcc ccagggggta agccgtcaat cgtgacggat acaggactct
    28621 cttccgcgtc ctcctcagcc tcggctggag ggtcttgata aaagaaccat ccctgcctgg
    28681 gatgatcgat aaacgtctta gagccgggag gatcagcggc cctcgcaaca ccggtcggcc
    28741 cccccatcga gcagacgctc acagagcacc acgctagagc aagcaacagt tttgtcgttt
    28801 ccatagcagc cacacaaagt cctcgccaac ggccggcacg attttgccga gcccccacat
    28861 catgagtgtc cgccccagag gctgacagca cggcggagga attccggtgt tcggaatagg
    28921 ccacagtaat tgaaacttgt actggctttt cacaatgaga gctgtcgtaa cgtcgtcaca
    28981 aagcaacggg ccattggcgg acgtccaaaa ctctgtcccc gcacgggcca gtttggctaa
    29041 taaccgtgtc atcgacaggg cagccggctt gaggtctcca tccgctcccg agacatgtcc
    29101 ggtcggcgga tacatgaatc caaagctccc ggcacaccag aacagcggat caatcgggaa
    29161 gccaaccgct gcagccacgg aatcggcagc acaggctgct aacgacaccg gattggccag
    29221 gaccaaggcc tctggaaaga ggatcagtga aatcagatca tcgtcccaca agggatcgat
    29281 ctcggaggca tacgccaaat cgatctcgtc tgcgatcccg gtaggattca gacatgcgat
    29341 gtccaatccg atgccgacga tggcccacag gggaaaaatg aaataatgca catgaaaaaa
    29401 tgcgtaattg agttcggagt ccatctgcgt cgtagccatg cctcgcccga tgtaactgaa
    29461 gcccccggcc aaggacacgc cgagcgtcgg gaaacacagg ggatcgtgta ccgcctcgac
    29521 cagattcttc ggttcccaga tccccagcgg taacccagga atgcagatag gcccaagaca
    29581 aacgcacccg cacaactgcg ggggaaaagg ggcgcggcct ggaccgacgg ccggggcggt
    29641 aaagtcttct cccgggttaa acaacgtgac tccgctgaga gaaataggaa acatacattc
    29701 ccaacacgtc gtcgcgagac tattcgccaa cacagggttt ccgattgtgc acacggcctc
    29761 tgccgtccca gacggccaca gcgtcgccac gccgatcagc acacaactca ggcagaccag
    29821 caagaacacc tgccggatcc ctcgcatcga ggacctagtc atgaatcgcc acctcctcca
    29881 cccgcagatt tcggcctagc tgagagacca cactgggcac cgctccgacc cccatgcggc
    29941 tgacgagcag aggattcgcc caatagacac gttgatggag ttgctccatt actttctgca
    30001 cgtcgccgtc agagatgatg agtttcacga acttagggtc atgctccctc aaatattgtt
    30061 gcacccaacc catctgtgcg ggactggtgc cgtcaaaaaa cacgagggtt cgaatccacc
    30121 gcaccttgtc gaaggggttg atcttcgtgc cggacttgat cagcaccgtg cccgtggtgg
    30181 gatccttgat atcctcgggg tactcgacgg tcggatcgac gaacgccact ctggcctctt
    30241 cgacatgcgg tagggccagc cctggcccct tggcgaaatg ccgtttgagg gcatcgccgg
    30301 aacggcgttg aatgtccggc caatttgcgt tcgccactcg ctccttcatc acatcaatca
    30361 tgtcccgctc cgcaatgtcg gctccacgtg cgtcggccca caccttcaca ctccgttcga
    30421 tggtcggaag cagtggcgga gcttcttcag tcggtccccc ctggagaaac cctcgatgcc
    30481 ggcgctcgaa ccattcaccc catggcttca acctcgcctg ctcctgtgac aacagcgcca
    30541 ggagatgtgc gaccgaggat gccccactcg caaccatgaa cttctcgtcc ttcacgaaca
    30601 ctagggccgg aacctctcct gcaccgactg tccggtagat caccggatcg atcccgattc
    30661 cgaccagcag ggagtcatcc gggcgttccc cggtgaagag cacccgctta atccgatcct
    30721 gcgtaacctt gaacgactcc tgtaccagtc cgcgcagcag caccttcgcg ccaagcagtt
    30781 tggcttcccg caggtagtct ttgagggtgt cgtccggtaa cgagaaggag agcagaaggt
    30841 agagtccgtc gcgacgcatc tctttcggcg attcctgggg gcgcccctgc cgtaacgcct
    30901 ggcgcgagta ccttgcgatg tcctgagcca tttcctcagg ggacggttta ctcgggcggc
    30961 ctgtcgattc agagagcgcg ggggatccga tgctcaggag ggagagcact acggtcactg
    31021 ccatacaggc gataacactc gatcctttca cggtacctcc atatcagaat gtctgtatgg
    31081 agtattaccg acctcgctag atgagcgggg tcaccgtacc gagaatatcc gcctgccgaa
    31141 tcaggccata gtaacgagag tcaaacgatc gcgggtgtgg cagcgccaca tagtattgcc
    31201 cttcgggaat agtctccggg agcgtggcta ggtcgatccg ttgcccgtag cgatcgattc
    31261 cgattccccg ccccaccgat tgtccgttga tatagacttc attatcttgg actagaatgt
    31321 ggtcacccgg cactccgact atgcgcttca tccaccgctt tcccacgtcc gcgtagtcac
    31381 ggtgtaagcg gcccggctgg acacgttccg catagtcttg ctgcatgatg aacgccacaa
    31441 gactccctcg ccccacggat tgaccttttt gaatgaggaa gacattatgg tggggccaac
    31501 tcccgctcat gcgagcggca acttcatacc agggagcgat gacaccggca gcaatgatcg
    31561 tcccgcagaa cagcatcaac cccacatgca cccaccgatg ccgatcaagc cactcgagga
    31621 ttgtcggcgc ccgcttgagc acgttcagca gcgataatgc attgaatggc ttcatcgatg
    31681 gagagctcct ttggcccctc tttcctcacc cgatcaatca gcgccagctc atccggattc
    31741 gtcgtaaaca acaactgcgt ttcccgtggt acagtcagtc tgcaaatacc tcgcccaaat
    31801 ggggtgatca tgaaaatctc tgagtactcc gtggtttttg taacagagga caggagtcgg
    31861 tattccatct ccgacaacac aatgcgccca ttgttccgaa acgccgcgat gctctcggtc
    31921 ttttgtttga gcagtcccac aaactccgca ttttcgagca aataacgccc cacatcccgc
    31981 atcttgtcgt agtagtcgtt gatgccctgc gtgatggtga tcatggagcc acccgacttc
    32041 cggactcggc gcgcgccggt ctcaaaaaaa gccgcggtat tacctccctg agacaacaaa
    32101 tcccacgcct catccagaat gacgaatttc cgccgtccgc ggttctcttt tttgtacatc
    32161 accgcctgaa tattcatctg catctgtagg aggatcaccg atcgcaattg cttctgctcg
    32221 ttcaacccat caagctcaag caccacgaag tcgttctcga aattgacgtt gttcgtgccg
    32281 ttgaagagat gggagtacat gccgcccttc gcgtaggacg ccaacacgcg cgccaggtct
    32341 ctggcccgag gatccgtcat gcccctcaac actcggtgca ccgaatccgg acagccctca
    32401 cgcccttcca cctcaagcac acgcatgatc gcttcttcga tgaacgagag ctcgacatcg
    32461 gacagcggac gcgaggggga agccatttgc gcatgcagcg ctttgagcgt cgccaattcg
    32521 tccgcgcggt cactcggatc gtcatcgcct ccagcactgc tcccccagtg caccagcttg
    32581 ctgaacggat tgaaacagag gcgttgagcg ttggctccgt actggatgta ttgccccttg
    32641 agcaactcca caagtttgac gtagctgaag cctgcatcga tgacccacac ctggccacca
    32701 atgcccaagt agctcttgat ccaattgttc atgaagaacg atttacctgc ccccgacgtc
    32761 gccgcgatag ccatgttgta attcgtctgt tgattggcaa agacatccat ggtgataagt
    32821 tgacctgacc gagatgtcag cagcaccact ggagcgccgc cccccttcca atccgccacg
    32881 atagggcaaa tatgcgccgg gacctcggtg tggtaggtct tcatgcgtct gagtccgtcg
    32941 atgagggctt tgtcatccga tggtaagccg aggggtaacc ccgtcatgaa tattttccac
    33001 ccgatgaagt gatcttcctg cagagtgata tccttggcac gatatagggc tcgaagagtc
    33061 cctgtctgct cctccacgtg acgcggggtg tccccataca ggacgacttg gaagtaggat
    33121 ccgatgagct gtcttccgtt ttccagttgc gcttgcacac cgttgaaatg gtccaatttg
    33181 aacctgaggg agggaatcag acccagaaga aacccactgg cttgctggtt gattacgacg
    33241 tgcttctttc gcaacttgcc tttcgctttg ccctgatcaa atttcaagac attcaacgtc
    33301 aggaaaaacg gacaggtgat ttgatcggtg aaccggacca ggctcccgat caattcgcga
    33361 ttgttgccgc catgccactg tacaggccat gactgtacgg tgagcgtctt gagaaacttc
    33421 ccgtccaact caatcatctt ggtgcggagc gtggccgccg tatcgagacg gaccgcctga
    33481 tcccgtatcg gcagttgatc gctgtacgcc ggcggccgac tctcccagct gtggcccggg
    33541 ttcagcagca catgcagcac attcaacaat tgcagtggcc ctacccgctc aggcagcaat
    33601 cccaaggtgt tcaactgtcc ctcgatgccc gacaccgctt gatgtatccg ttcatcttcc
    33661 cacccctgcg cggtcgtctc ctgggtcggg aaggccacgg atacatacac ggtgaaatct
    33721 cgggggcgta acggtggatt ggacgtcagc tgtgtgtctc gagcatgcag atagaactcg
    33781 gcggtcttcc gcgcatcggc cacatagcgt tcccctgcct gcccccgttg cgcgagcaca
    33841 acgtgatggt ccagataagg ctcgatgatg ggagatgcat gcagcataaa ttgaaacgcg
    33901 gttccgatcg gcaggtctga ctcgaagaga ttcgtcagct tctgaataga ctcttccgac
    33961 aaccccagta gaggcttgct ggcaaacacc acaccgatgc gcccatcatc caagcgatag
    34021 acctggttgg tgtcatccca agcctcatag ggcaaccaat cactgatggc cgtccgctcg
    34081 accagctgct ttgccttcca ctcaaggatc gtcgacatat tcgctcctct ggggtccttc
    34141 cactactggg gtggcaactg gccatcatcg gtcaggtaat tggctgctcc cccggactgg
    34201 ggaagctgcg gaaagccctg cggcatgatg ccgccagaac caagaggatt cacgggcatc
    34261 ggcgccgcgc gctgccctgc ttccggaatg ttctccgtca tggcgttcac cgccgcgttc
    34321 ccactggagt tcctctttcc gtctcgtttc tcgaccatcc gccccattct cggatacaaa
    34381 tcttttccat gccacgaact cgatccggga ctcacaccct gcgtcatatg cccgaaaata
    34441 aagtccggtt cgtcgacgat gagatagtga cgcgactgat cgtggaggcg ttttttcgta
    34501 tccttccagg gaaataccgt aacacggagg gccgaggccg gcgtgatcac cggcttacct
    34561 acagcactgt ccggtttgcc gctgaagacc atcggagacg gggcctgacc aggccctgaa
    34621 ttaccgctgg tgtcgtgtga ggcgtccttt tggtctttcg tgtattgcgt caccttctgg
    34681 ccggcagcga agcgcttctc gtaggcttcg gagactgatt cacacgtcgc ttgatctgga
    34741 taccccttac aactgaaatt ggattcatag ccgccacagc ctgcgaagag gacacacagg
    34801 ctcatagctg ccccagggcc caagccgtgc catgaaccgc cttgcgtgat acagcgcctc
    34861 ataaacccag acatcatgtg gtctgcctcc ttgagaatcc gccccgaccg tattccctac
    34921 ttctgctcca tctctagctg caatagcgcc aacacttcac tggcaggacg ataaccggca
    34981 aacgattgac cgttcggcaa gaacaccatc ggagtggaat gcacccccag ccgcttcccc
    35041 aatttgatgt tgtcgtcgat cggatgactg caagaccccg cttcccccac caccggcttt
    35101 gacatcaggg cccgccccag cgcctcagct tggtcggaag cacaccaaat ggcgacggac
    35161 ttgcgatacg catccggatg cgtccgcacg agcggataca gcaccacggc taccggaact
    35221 cctgcctcga cgagcttttg cacctccgga tgaaactgct ggcagaacct acagtctgga
    35281 tcgtcgaaga ccagcagcgg gcgatccaac ttcggttgca tcggagacag taggatggtc
    35341 ttcgacgcat ccatcccggc cagcagcgcc tgttgacgat ccctcacatg ctcttgcgtc
    35401 acattccggc ccatctcgat atcgaagagt gccccggaga gcacgtatct cccggagtca
    35461 tgcacgtaca tgaagttttc cactcggtca ctgtcgctct ctaccaaaag ccaccctggg
    35521 atttccatct tggacactcg cagcactttc atgccgggaa acttctgttc gatcaccgtt
    35581 ttgatgggca ttactgtatc atccggttcg gctctcaaat caggctcaca caccaccgac
    35641 acacatatcg cacacaacat gcccgccatt agctgctgat aagattgttt catcgcaaca
    35701 tgccctcttt tgaattactg gaagaacccc tccaactcgc gtcccgttcc cacgataaaa
    35761 tccacttccc gctgcgcgtc gatttcgatc accgggaaaa tggaagccgc catcatgctg
    35821 taatgacgag ccaacaactc cgcggcgcga ccggcgccac cagccatccc cgacattgcc
    35881 accggcccgg ccggcggtaa gttgaaggct tggctcgggc tttgggcaac gaaaaatccg
    35941 gactgatagg gcatgaaggc ccgcgagatg ccggacacaa acccagcaag cagcgtgcgg
    36001 cccaacaatg ccccttcctt caagaccact cgcccacgca tcccctgttt gccatcttcc
    36061 ccactgacaa accctttcaa ttcgatatcg accgcctccc cgttcttttt cacgcacgag
    36121 atggcgttgc cgcgaatcat tacgcgttcc gatgacaaat ctcccaagcc ttctcccacc
    36181 aggaaacaat ccttcgcatc aatcctcgcc tcatttggaa gcttcatgat gtcgcgcacc
    36241 atcatgagca ccgggtaggg gctgtttaca gcggcccccc cgccctgcgc ccccgtcgaa
    36301 cgagccggcg catccatgcc ggagagtaac ttgaccggga taatcgtccc cgtgttcagc
    36361 catagtttct tttcgtgctt tttggcgcca ggcactgtct ctggccgaaa cacctgaaac
    36421 gcctcggtcg ccccgcccgc aaccccagga gcaggaagcg ccccgcctgt cggtttcggg
    36481 gcgctttgca cgggaatggg ccccttcggc gctggcatgg tcgagacggg cgtcgcgctc
    36541 gctccaccca agggattggc actgagcgga ggaggcagcg ggggcaaggc gccactcttc
    36601 gtcccggcta aggccccacc accagaacgc gccaccgact caatccgctt ttgcagatcg
    36661 ctcaactgtg tcttcatttc tgtcttgtct cgcccatcct gctccaacag tctacgcatt
    36721 tggtcccgct gctgctccaa cgcctcaatc ctggcccctg cgtccttcat atagatttct
    36781 ttgtccgtcg cctcacgatt gatcgacacg gccttgacgg gagacttgcc acggacaatc
    36841 cctggttgat tgcctcggaa ttgctgcagc accatgctca ggacgatgaa tccacccacc
    36901 aggacaactc ccgccaacag ctttgaaggg gccggtttct ctggccgaat cgggtcgcca
    36961 gggttgagaa tcgcgctctc cgtcggttta atcgatgcca tcctaggtct ccggtctgag
    37021 gttagccctt tgcgtctgga tccactttct gctccggctt gggtttgggt ggttcagacg
    37081 gagcgtcatc cagcagatac accaccactt cttcgcctga ctcgaccacc tcgcgattta
    37141 aggcgatcgc tttttgagtc ccagtcagga attcctgttc ccgcaacgtg tatttttgtc
    37201 ccgaggtatt caccaatcga tacacaatca cccggtactt cggccctcga tactcgactc
    37261 cctgcaccac tttcagctca agccacttcg gatatcgttc ttcgttcatc gtcgtctgcc
    37321 gatacccctt gggcaacgcg ccacgcgcag ccatctgcag tgtgtcgagt agcccatcca
    37381 catagtcccc ggcttggcgg gcaggatccg aatcataggg cacagtcccc gcgcgcctat
    37441 cctcaatatt gactgtctgc gcaccgagcg acgcgctcac cacaacttcg aagacgtacg
    37501 tttgctcacc ggccatcacg accagatcgg ccgggccggt cttgacctgg acgatcaggt
    37561 tgcgcccttc cggtttggcc ttgagatccg ccctagagct cgtcgccatg gtgatcggcg
    37621 cgggaaacgt cagcacgttc aaatcccttc cggacaacgt gaggggcgtc gaaccttcat
    37681 cacccaccac caccgccgct cctgctgacc cggccctgga taagcataac agcaggccaa
    37741 ggacgcacat cgccatcgcc cggtgctttg tgtctcgata cacgctggat gtcatacgtc
    37801 tccccttacc cctttgccgg atctgattga ccatcctgca gcgaacgatc caaatgaacc
    37861 gctgagagcc cgaccaccca cggcctccct tgctcccatc gcatcgaaat gcggtactcc
    37921 cgctcctctt cccgaatctc actcttcccc acaaatcggc gctcgatccc ctcgaacacg
    37981 gccatatccc cgtccacgcg gctggctgtc acgaaaaaca cccgactgag attgttggtc
    38041 ttcacgtaca cggcttccgc tccaagagac tcggacatca gcccatagtg gctcgatgga
    38101 acccactgca ggagctgacg atggtgaaaa tcaacagagg cgggcgtgac attggcaatc
    38161 cacggcaaca tggccatggc aatggacttt ttgtaatact cgtcgccacc catcgcattc
    38221 gcccaatatt cctgggtcgg gttgatcggc accacgtgca ccgcgacgtt ctgccgaaag
    38281 aatccattca ccaccaacgc cacagcgaga acgatggcca gggccgcgaa cacaagattt
    38341 aaaacctggg cgttatgcaa tttggatttc cacaactcga ttgtcatcgt cgttactcca
    38401 tcatctctcg ggtggttccg tgcggtgccc ctgtcagttg aaacgtgggc acaccagacc
    38461 aataggccca atggaagagg aatccctcgg acttcccgtt cttcattttc gacaacacca
    38521 acgtgctcac gatcccgacc acaatcaggt aggtcaggaa cttcgtgaca atgccgagaa
    38581 agatgcacac aaaaagcacg gccaactcat caatctccca ccagaagatc tggggcaggc
    38641 tatcgagata tcgtggaata aatgtgccgt tcatcagtcc ctctcctact cccttgtgac
    38701 ggcggggcgc tgatcagccc cgccgtcacg acgccttacc tgcaacgcgc gcttacatca
    38761 gtcccgtggt aatggtcggg atgatgttgg ggatataaaa ggcggcggca gccacaccga
    38821 acccggtgaa catgccggcc caacggcccg ccatcgccga tcccaagccg atcgctcccg
    38881 cgaccagggc caacaacgtc ccaagcgacc cctgcatata gttggtcaac aacgtgacca
    38941 aggggccaaa cgtcgcatcc gcacccgcga acgcgggcac ggccagcacc acaaacgatg
    39001 ccaacaccat gagccccaac agaagactca ttcgccaaat gtctccggcc actttcctta
    39061 agccatcgcc catactcaag cccctcctct ggctcgtcgt tcacagttca gaagactgcg
    39121 gttgcccaac cgaatgaccg ccctcagttc attccagcat tgtcatgctc ctctccagcc
    39181 tctcattatg gtttccctga gtcgtgagtg gcaccagctg acaccacagg tgttaccgga
    39241 ggtgcttccg atcgccgctc aacctcggag gaatctgcac acctgccttg caacagcatc
    39301 atgacctcca cgcgtcgact ctccgcgtgg ttcggcagga aacagcagcc tcctcgaccg
    39361 accacttcca cctgaaccgg ccccttgacg cgcgcctgca gcaccttcgc caccgcctct
    39421 gcccgcgcca tcccgagttc ctggtttgtc tcccgagatc ccgtcggatc agtgtatccc
    39481 tccacccgca gatagaggat ctcctcgatg cgtagttccg ttaagtcccg ttgcgcacgc
    39541 tctgacaggt cggccgaccc cattgcgaac gagacccttc ggtgccgtac ctcaggctcg
    39601 cgacggcaca cgaccgtggt cgggcttccg gtgggaccag gaatcacagc cttgtccgga
    39661 ctgcttcccg gagcgcggga catgtcaccc ggacgagctg ccagtgtgcc cgacgaaaac
    39721 gtcggcaacc cctgtagccg atggatccct gctttgatcg gtttcggcgg gagatccgcc
    39781 accgtcggct tgttaggtac ctgcgtgagc ggcgtcggtt ctggacatcc catcggacga
    39841 gacagcagcc attgcggacc agattggacc gagaccaccg ttgtcggaaa ggtgtagccc
    39901 tgtccgcaaa gtcgcgttgg atctgacatt gttggcggca cggcaggtct cgtggtacag
    39961 cccccgacgg tcagcagtcc cacacaggcc agaccggccc acgacatgta agtcggccgt
    40021 ccccctgtca tacttccccc tttcaccatg gctcccgaat ggccaggctc atccttggta
    40081 ttaccgactg ccacggtgtc tcgcggacga acagaagatg caggatgtcg aaggggacct
    40141 cgtcgacgcg acgaagtagg cacgagggga cgcaagcaga tcaggaggcg atcagatcgc
    40201 caggagccac gcgtccagcc cagactcgga aggacccgtc ggtcgatcgt tggaataacc
    40261 gagcgcactg aagacactga tgaagacagc taacctctta acatttggat aatacgttcg
    40321 agggaaggga tctgacacat actcgccact gcgacggcaa tccccagggt cagggtctgc
    40381 gtgggcttgc cgtcgtatat ccacaccggt atacttgatc cgtgaaaccg ctgcgctttc
    40441 ttgggaactc gctggagcgt ctccgacagt ttccggaaga cgctcggcat gatgtggggt
    40501 accaactaga gcaagtccag cacggcaagc agccaaccga tttcaaacca atgccgtccg
    40561 ttggaaaggg tgtcgaagaa ctacgggtat gggatgactc cggcacctat cgcgtgctct
    40621 acgttgctcg gttcgcagac gcggtgtacg tgctccatgc gtttcagaag aaaagccggg
    40681 cgacgagtgc acgcgacctt gagcaagcca ggacacggta cgctgaactc aagcgaggga
    40741 aacatgacta agacgatcca cagtttcaag agtgtgtggg acgcgttggc tgatacgccc
    40801 gaacaggcgg cgaacttgcg tgcgcgcgcg gcactcatgc ggcagatcac caaggtcatc
    40861 gagacgaacg ggtggacgca ggcggaagcg ggcgccacat gcggggtgac gcagccgcgg
    40921 atgaatgatc tgctccgcgg acggatctcc cgcttttcct tagatgcgct ggtgaacatg
    40981 gcggcggcac tggggcgacc ggttcgcgtc gaactacagc ctcccaagca caggcgagtg
    41041 acccggacgc gtcgtttggc ctaagcctgg cagcgaaaag cgtttttatc gtgcgctcat
    41101 acggacagcc gatcatcggt tcgacgcccg cctcgcgaca ataaaaaagc gaggcagaaa
    41161 gggagaccac catggcaacc acactgcatg agaagatcaa aaaactgggg acgagacgcc
    41221 aagaaaagat tcaagcacgg gctgcgcaac tgattgctga agaacagtcg ttacgtgagc
    41281 tccgggaacc ccccggttga cgcaatctga cccggtgcgc agggcctcaa tccgctccca
    41341 ctgtcccccg aaggcactgt agatcctcgc ggactcatac aacatgtgcg gacgaataca
    41401 cttcccgacc gcagtggaca tgcggtacgc gatcttcacg ataaccgcat ccccatactg
    41461 gctccgctcc agcgcttcag ccacgatgcc gccaagcaga aagcgttcgc gtatcaattc
    41521 tcccgccaca tacgtgatcg acctggcaca ctgttcagcc cgttgcacca gcggttcttg
    41581 cccgctagaa cgcatacttg acatcctccc cggccttcag gccggggagg atgtcaagga
    41641 ctcgtgccgt gatattccaa cctcagggca cggtccaggc tcggagatgc cttgggcgct
    41701 accgtgccag ctccgaccgc acgcaggctc atgggtctgc atcgccgtat cactggccag
    41761 cgcctccggc tcatccacca ccccccccca cgctaataca ccatacagac tgtcgcctcg
    41821 cgtccacgcg ttctgccgac atattgcatg cgctcctcga ccgcaaacgc cccacactcc
    41881 cgttctggaa tgataaacac cacgcgctca tagcgactcc cctgcgcctt gtgcacggta
    41941 atgcaatagc tcagccgcag ctcctctgcc ctgagcgcaa cctcacgccc ggagtccagt
    42001 cgcaatgcga tcgtgtcacc catactcccc agcacccgcc cttgttcccc attgaccgct
    42061 cgctggccta tatcgtatcg agtcttggtc acgatgacgc ggtcccccgt tcgaaacccg
    42121 ccatgacacg gcggaccatc cggattcata atctcctgca gccaccggtt cagccgatcc
    42181 acaccgagtc ctcctgcata cacaggagac aacgcctgcc actccacccc tgtgtcttga
    42241 tagccgcgca ctatttttcg taactgatac agcaggagcg cttcggaggc gaagcgaatt
    42301 acctgaacct tgaccagcaa ggaccccttg gagccttctt cgcccaaccg caccaccggc
    42361 aggactgtca acaactcagc catcaccgga gtcgctccca ccggagccag ttgctgggga
    42421 tcgccgatca acacaacctg cccccgacag gcgagcagca atcgatacaa ggtgtgccag
    42481 gtcaacatgc cggcttcatc gaccaagacc aggtcggcat ccacagtgga caccgagtag
    42541 ccgccgcccc cataccccag cagcttatgc agcgtcgtgg cgtcctcccc gaggagggtg
    42601 gccgctttcc cggtcatcgc cgtagccgcc acctttcccc cttgctcgcg cacctgggtc
    42661 atgagggatt tcgctaactc ggtttttcca ctcccagcac cgccagtgac aatcgaatac
    42721 cgataccgca gcgatctggc ttgcacgctt gaacgtgcgt caccagagtc ctgctgattc
    42781 gcccgaatca tggccagggc tttctttcgc gcgaaataca ccaccggcga aaaccacaag
    42841 ccctgctcga atttccccac aagccccatc tggatgctca ccgacggcgc caccttgtct
    42901 gcaggcagtc cgatcagctc tgcgcaacgg gccacaagat gcgcctcaga aatgcctccg
    42961 accccttctt cgtcggctcg cgcgagcacc tccatcaccg cggccttata gtgtccgtca
    43021 ctacacaact catgccctaa cacctgcgcg gccgcgaccg tagcctgaaa gggaaggcgc
    43081 cggcggcggt acagcagaaa cggatctgcg gagatggccc gcgccgatcg cagtgtgccc
    43141 ttcactatcc cacgcgccac tgctttccac cctgcacaac acagccattc caccaggggg
    43201 cctaacaatt ggcatacctc ctcgaaccga agtggtgggg tacgacgcac gccgaggacc
    43261 cccagcaact ccgacgggct cgcgcaggct tcaaatcgtc gtgatcgtat cctggagacc
    43321 atcagaacgg gatcggcgcc attggctgtc attcgccccc agcactctcc cacctcaccg
    43381 accgaaggca cgggaatacc gtccggaaca aacgcccgat gcttgcgacc gatttcgatc
    43441 cagtcaccag cccctcgtcg cgcacgggtc cgactcgtga tcaccccaac cacctgaggc
    43501 atttcctcag ggcagctcgc caccttggcg gagacgtcct tcccctgcat actcccttgg
    43561 atcgatccgt agccgttcat ccttctccct cccccattct cagccatcac acactcgtat
    43621 ccacaactct gtgacatacc gttttgctcc caaaacggta tgtcacagca tcttcacatc
    43681 agcgaaccgc acaacctctt ggtctatcga cgcgctacac gaactcacct ggaggtccta
    43741 tgctcgctga actcatccat ccactcgctc tcatggaaca tcaggaatgg gagtatagac
    43801 ctcggccatc gaacgccggc cccgaccact gcatccgcaa actcacgtac catgctcgac
    43861 acgctcccgc gaaaagtcca agcggccgct tccttgtcgt cctcgacgac agtagttggc
    43921 atgaagaact ggtattgaac tggatctcca agagcacgtt tcatctccac agccaacagc
    43981 ttgaattaac ctgcgcgacc acgctcttcc agggtcgacc gttcccaatc aaaggtcaca
    44041 ttgacggcgt catcacggac ctcttgggga ttgatcgcct cctggagttg aaggctatcg
    44101 aacacttcac cttcactcgc tttgcagaag ggaattaccc gataaaccac ttcgtacagg
    44161 tggtcttcta tctggttggg ttggccgcac tgaatcctca catcgacgaa gcgctcctcc
    44221 tcatcaagaa caaaaaccag agcgcgttcc tcgaatatcg agtccgctac gaccgcgaca
    44281 acgaccgctt gaccgtgctc gaaatcacac acagcacagg cgcgcgcatc tatcccaagc
    44341 aagactttgt gggcttatat caggaggcac tacgtcgatt tgaagaggtg gaacagtatc
    44401 gggatcacgg cacattgcct gcccgcccat atcccaatgc cgacaactac cattgcgact
    44461 actgtccgta ccagttttgc tgggaaggga tcgaacaggt ccccgttgag ggaatcgcgg
    44521 aactccgcac tgaactggtg ccagccgtcg aggagtatct cggcttgcaa accgagctcg
    44581 cagcactcac tccgaagcaa aagcggttgg acgcactcaa acagcaattt aagttggaat
    44641 tgaagacaga gggtatcaca cgcgcggaag ggcatggcta ctacatcgca tcgtcaccgt
    44701 atgaacagag caaattagat caatccaaac tgtccaaagc cgtcattgaa cgcgcgaccg
    44761 tgaaagaatc ctacagccgc ttctcagccg gccgactgtc caaaggcatc gacaccacca
    44821 aatcgaaacg tcggtccccg ctcaagaagc cgaaaccgac gactcaaatc gcgtaactgt
    44881 attcatgcgc tgattcacat tagtaggagg catcatgtcc gacgcagaaa aacccacgtt
    44941 cattcccatg aaccgcatca acggcatttc taaccgacgt cgcatgccca tcatcggcaa
    45001 gatccgcctc ggcgtgaaag tgagcggccc caacggattc taccccaagg aaacgcctca
    45061 ttttgtggtc cctgaagagg tcgccaaggt gttcggggcg caacccaacg agctggaggt
    45121 gatgttcgtc tccaacgatc ccacggtcaa ttgcccgcag gccctacgct ggtacggaga
    45181 agccgccggg ctgcagtgca ttggcaacaa tcaggtggct cgtcgcctga accaagacac
    45241 ctggaaaacc gaatcccggt cgtgtccctg cgaaaaactc gaatccggag aatgcggccc
    45301 tcgcgcgaat ctcatcttca tgatccccaa ggtcagcagc ggcggtctgt ttcaaatcga
    45361 ctccagcagt atcaactcga tggaaacgat taactcctat ttcgatttcc tcgcgcttac
    45421 ccttggacgt atcgccatga tcccgctcaa actgcgtcgc gttccagtca ccagacgccg
    45481 cgaaggccgc ccgaccaccc actatccgct cgaattgcgc tatgaaggat cagcggacga
    45541 gactgccagg tatcaacagg agacggagac ggtcctaaag aagatcaaga tgttggaaat
    45601 ccaagagccg atcgaaatca acccggtcct ggatccagga gcgcacgttg taatggaaga
    45661 ggagctagag gacgaccccg gtcctgtagc agccgaggtt ccgggggaca ctccgtccga
    45721 gacgcacgac cccacacctc cggcgatgtt cacgccctcg acagccgccg ctaaagcgcc
    45781 ggagcccacc cccctgcccg ctggcccact catctcagtg gcccagacca actacctgct
    45841 tcggttgacc aaggccttgc ccctcacaga ggatgatctt cgcatgaagg tccgcgcact
    45901 cacgcaacgc gaagcgtcgg ggctcatcaa gagactcatc gctcaggact ttagctattt
    45961 cacgtcgagc ccaggcactc ccccgatcac gcctgcgaac acaggggccg tcgccgagtc
    46021 catgaccgat cctcagttca agaagatcat gggcctcgtc atctcaatgg atatccagga
    46081 gacacacgtg aaaagtctca ccagcggata tactaaacga caggcctcga ccctcatcga
    46141 ccagctcgtt ggtggagatt tttcagcatt ccaggagatg aaggaggagg agctgccagt
    46201 gcttgtcact ccagaatccg caccagtaga agagaaagta gagttctaat tcgctggcag
    46261 acagacacgg agaatatcgg gatgactcag ggacggctgt gagccgtccc tgagcactac
    46321 tgccacgggc gggcacacgt caagaatccg ctactcatga gccgtaggtg ggtgcatgcg
    46381 ctcagaaacc ggaaggctca cggaggcctc cccaaagcgc agcaactctt cctgggtttc
    46441 tgggcgcagg gatccaaaga cttgaaggat attcacacaa ttcggattgc gtccgaacag
    46501 cgatctcgcg gattcagata gatacccggc ctcgactaga tatgtctcag acgactgtcc
    46561 taagacctgg gcgagccgct ccacttccgc ttggctcaag cgaccgatcc ccgatagcac
    46621 ctgcttgaat cttccaccta gctgtacctg gtccgtcacc ccagctagct cctcatccga
    46681 atactcactc aggtcttgct taacccgctc caaaaaggca gccggcatgc cagtaagaat
    46741 ctgatcatct aagagttctg cgacttccgg ggaacacact tcacgggctc gggcgaccaa
    46801 cagttggtag gtccgccgtt ggacatcaag cggggatcca cccagacact gcgcaatgcg
    46861 ccgcaacatg tcgtcactgg gaagaatctt tttccgccca ctgctctttc ccccttcaat
    46921 aatcgagata tgcgaggccg tacaccccag tttctcagcc aactcagttt gagtcagatt
    46981 ctgggcaatc cgcatccgga ccaacaagtc cttgaccaac accatcccca ccacactctc
    47041 cccgtcgggc cccgacgggt acccaaccca cgctgatgga gtgattaaac gcttgttcgc
    47101 aaattatagg taaaccccaa tttattgtcc atacgcctat ggaggttact ctaccttccc
    47161 aatttcaacc tccctcacgg cctgcgtata gtcagactga cattcacgag gcagtaacac
    47221 attcatgggc aaacaaacat ttgtttatat tcatcatccc tcgccgctcc ctggaagaag
    47281 ggactgccgc acgaatgtca caccacttac gcacggaacc atcatcggct ccatcagcgt
    47341 tgtcacgcac cctttcattt tccgagacgc atggtctcac acatctaccc catcggaaag
    47401 gacaccatca tgtcgtttcg aacgcaagct caacaactcg ccatcgattc ggacaatgac
    47461 tcaccgctgc tcaaactgtt tcagcagagc aagcccatca tccactcgat cgcgaaacgc
    47521 tactgcaatt tagaccgctc ctatgaaatt gaagacttgg agcagatcgg ctttctggcc
    47581 gtcaggaagg ccactgagaa atgggacttt tcccgtggaa acgcccaatt ccaaaccttc
    47641 ctctatcgct atattcagaa agcctaccaa agcgaattcg atggacggga caaactggtc
    47701 gaaatcaccg acaaggacga tcaatacgtc aagacactgc cctattccac tttcatcaaa
    47761 gagaagcggc gcttacacag ccaaggcttc aaaagtcgta tcttccggcg gcttgtccct
    47821 ttgacgaccc acgaaccgcc tgtcgtccct gacgcctcgg ctgaatcttt ggcacagacc
    47881 gatgtgcccg gcaccgacga cgtcttgggt ttcacggcta ccgatcggtt catcgaattc
    47941 tcgcgcaaac gcgccagagc gatccccgac ccgactgctc tcgatcacga cctgctaacc
    48001 acgtcggaac agtcgatcac gggatcttcc tccggcagcg gcaaccacgc tcgccaggca
    48061 cggaaacgac ggacctatgc caactgatat gacacggatc atagccgaaa ccatctgccg
    48121 gcaggtggga cttcgcgcgg gtccgactcc gatgcccgcg gagcccgtgc tccccatgag
    48181 cgcgttccgg gacatctgcc ggggccacaa cattccctgc agtgattgga aactctatcg
    48241 ttggttaacg ttggaacggg agagtctacc cagtccggta caagccatcc tccagcatgc
    48301 accggcccag tggtacatcg ttaccacccc ctctcaaggc cgctatcgag gtcggcaata
    48361 tgtcgttcct ctccgctatt tgtccgagat ccatcgcgtc tttgcgatcc gacgacagct
    48421 aaccaagaac ctcgccgagc cagaatcctg tgctcaacag attgctaatg gcatccttcg
    48481 ccgacaaccc acaatcacca gtcatcaact ccaacgggag cttcatcgtc gcggccacga
    48541 cctgtcccga cgtcacattt acagaatcat cgctcgtcac agccagcaac aaggagcccg
    48601 gcatgcagat catcatgatt cctacatctc aaatccgagt ccatgacgag tacgccacgc
    48661 tctttccgcc gctcacgtcg tcagagagag cgagcctgaa aaccagtatc caagaagccg
    48721 ggatccatga tcccctgcga gtcgaacgac agcctaccga cgcagcctcc gtccagccct
    48781 atgtagtact cggaggccat catcgcctga gcatcgcgca ggaactgaac atccccgagg
    48841 tcccctgcat catcgtcacc accatggaac tgaaggtcgc tgcgctgttc gacaatatcc
    48901 atcgacgcca attgccggac acccttcgtc gacagatgca ggatgaggaa cagcgttacc
    48961 gagccaggct gagggaacgg ttgattccag agattctgga tgtactcccc atgctgccgc
    49021 cggaagttca actccagctg acacaatcca gccacgacac tcagcgcgat ttcctctcca
    49081 agtgggtggc ccaagtgcag cgagtgggca ttcagcccca gtcggagcca cagcacacca
    49141 gccccgcccc ggtagagccg gaggctccgg acgcccccat accgaagcag cacaaacagc
    49201 taatccgtca gatgaatcag atgaccaagg actttgaacg acggatcgct ctgcagaaga
    49261 aggagttgga ggctgtccga gagcacaaca tctccctgtc tcgtcagctg cgggagcagt
    49321 cagatgaaaa ggaccaattg aacgaaaacc tcgcatcgct ccagcgacaa ctcacaagcg
    49381 aaaatctcga ccataaagcc gagcgcattc tccgcttgca gcacaaaggt cgccgccaag
    49441 gccccgcgat tgaagaggcc ggccccctga ttgccggact cttgcatacc cgcaagaccg
    49501 ttctagtact ccagacttac gcccagcaaa tgaccaccct gaccacccct gaccagacca
    49561 cgattctcga ccatatcaac ctcaccaacc aagccctggg cgcgatcgag caggtgctgc
    49621 cgaggccgac ggacccgctc cagagcatcc cccgaccctc gcggtcagtg aacggagatc
    49681 gctccgccga tagctagcga tacgtgcgcc ctagggagat ccccaactcc cttccctcgc
    49741 caacaggcat ttggacactt gttcatactc acaaatactt gtactggtga gctgcgacaa
    49801 tcggagaatc tgcacatgca gtgttcctct cccgaatcgt tcaattccgt cgatcaggcc
    49861 cactcggctg acgaccatcg gtctgcactc cgcaccaccc tctccaagat cgccctgtac
    49921 aactttgcgg cagaacaccg attggatacc ccgatcgaac tggccgaacg cctgatcctg
    49981 tgtgtgtccc tggccaatga ggatcgcccg ctcacgcgcc tcgaattgcg ccaaatcacc
    50041 atgatcaatc cgttcacgat tgatagcgca ctcatcaacc tcgtccagcg cgaggaaatc
    50101 cacatgttgt cccaacggac gtccgagcaa gcagtctatg ggcttacccc gcttgggcac
    50161 accgtgacag tctcgcttga ctcctggacc acgagcttct accagtatgc gcgctgcggc
    50221 tccgtcgccg gggtacgaga cctggttcac caactccagc aggccatccg gaacatgatc
    50281 gctaccgact cactcccgat catacgtaca tgcattgcct gcgaatactt ctcgtccgcc
    50341 gcccacgccc ttgatcctca aaccgccgga tactgtcggt tcttgcgcac gccgctttcc
    50401 atccagacga ccaagtctgc cgctcctggc atccacaccc cctagcccgc attattcatg
    50461 acgggtcgtc acgaaatcgc tgaggcgcgt cgcgagaccg gcgcgcggag tccggaggcc
    50521 ccgacgcgta ctcgtgcagt acgtggaggg tccgaggggc gagcccgccg gccgaaggcg
    50581 cgtcgcagca gcggatcagc ggttgcagta gaaccgctca tgaataatgc gggctagtgc
    50641 caccggcgac cctcgtcgcc ttattccacc aaagcctcag tccgttcaga tccagtaaca
    50701 cataggtact tcatgtccgc ctacgacacc aacacaacat ccgtgtttcc tctcccgctc
    50761 gatcctcctc taccggcaga ggagccgtct gagccatcgc ctctcccgct gtcgcctcgg
    50821 aaactcccgg actggctgca tgcacacatc cacaccccga acgatcttac ccaagagcga
    50881 cactcagccg tagagctcaa cctggccacg atctcgcccc tcattcagaa tgcgcaactc
    50941 aatcctgagc aactcgctgt ggtcaccgcc ccggccgaac cgacactggt tttggccggc
    51001 gccggcagcg gtaaaaccca cacccttatt catcgcgttg cacacctcgt cacccaaggt
    51061 ctaccccccg atcaaatcct cctcattaca tttactcgga aggccgcaga ggaaatgatc
    51121 cacagaacac aactgctact ccagcagcac tcattgaaga tccaaggcgg caccttccac
    51181 gcgatggggt tgatctggct gaaacactat ggcggcgcgc tcggactccc tcccaagttc
    51241 agcattatgg atgaggacga tgcgcaaggt ctcgtgcact tattgatgag caaactgtcc
    51301 ttttcccgtc ggaaagggtt tcccaaaaaa caacacatcc tcgccctgtt cagccgggcc
    51361 gtgaatacgc tccgctcgat tgcggatacc gtctcccgtg attttccgtg gctggacgca
    51421 cacacccctc agctcgtcgt gctctttcga gcctataccg aaacgaagtg gcgccaacat
    51481 cgcgtggact atgacgacct gctgtacctg gtctacaagt tactctgcct ccatgagccc
    51541 agccgccgcc gactcgggac gcactaccag gccgtcctgg tcgacgagta ccaggacacg
    51601 accaaactcc aatccgaaat tgtgcggctc ctcgggagcc accaccacaa cgtgatggtc
    51661 gtcggggacg acgcccagag catctacggt tttcgcggcg cccatacgaa aaatatgctc
    51721 gagtttcagg atgccttccc tagcgtgcgg ctctacaaac tcgagcacaa ctatcgcagc
    51781 accgccccga tcctcaccct ggccaacaca gtgctccagg atgcgaagga tctctatcca
    51841 aaacggctct tcaccgcgaa ggccggagga cagtctccag aattgatcgc ctgttattcc
    51901 gaacaggccc agagcgactt gctcacggag cgcattctcc aatgccatga tgaaggcatt
    51961 cccctccatc gcatggcggt cctctttcgc tcctccgctc actccaacaa cctggaactg
    52021 acactcgccc gacataagct cgcgttcgtc aagtatggcg gcctccagct caccgaaacg
    52081 gcgcacttca aagacgtact tgcctacctc cgggcggccg acaatcccct cgacagcaat
    52141 gcctggcatc gcctcctcct gctgctcgat cgcgtcgggc cgaagctggc cgagcggatt
    52201 atcgccacaa tgcaggaagc tcctcaccct ctggacgtgc tcgatcaagt tcctggtaaa
    52261 gccgaatcga ccatcagact cttaaacaac gtcattcgga cgacacagga cgagagcatt
    52321 tctctaaccg atcgtctcac cttcgtaatc aagcattacg aaccgatcct caaacgcgat
    52381 tatccggaag atgccaaagt ccggatcgaa gacctgaaac cactctgcga cctcgcacga
    52441 caatcgacca gtctggcggc gttcttggaa gacctcaccc tgaacccctc ggccacctcg
    52501 agcagcaaac atgcacaccc cgatcaccac ctcgtactct ccacaatcca cagcgccaaa
    52561 ggcttggaat gggacgtagt cttcgtcatc tgggtcctcg atggacagtt tcctccagtc
    52621 agaagttggc atgaagagca ggccctcgaa gaagaacgcc gcctcctcta tgtcgccatt
    52681 acacggccga gagagcagct ctttctcacc tacccaaccg agcactacca caacgcgtcc
    52741 aagcagaatc tcgactcgct ctgccggttt cttgcgccga ttccagatga catccttcct
    52801 agctacgaga tggatcaagc ctgatgaagc ctcccagcac attccgcttt gagttccgca
    52861 cccaatgcga acggaaggat cgaccctttg tggctcgggt cacctgggac accgaggcca
    52921 aatcgctggc acgcggcttt tatgccatcc aaattacgcc cgtgggtcgg ttcggagcgg
    52981 ttggcgaatg taccttcgac gccgcaatcg gggacatcgt ggaagtgaaa cgcggcgaaa
    53041 ccaaagaccg ggaatatcgc ggcttctatc tcgtcgtccc cagaggatat ttgatcctcg
    53101 tcgccattgc cgatgaggtc gatgacgccc tcaaagtcgt ccaatatgtg gccgggcagg
    53161 cgtcgcccca ggacctcgcg aagggacatt ggcggttcga atcggctgag agctcgtgga
    53221 agggccgacc ggcgcccatc gatcagacac gaatggaacc acatcccccg cgacctgtgg
    53281 cgcagttggc cccgcccgac aagcgaccag ctaaacccac gagagagccc gctgccccca
    53341 catctcgaag cagcaccacg cgctccgtca cacgcatctc cagacacaca cccccgctga
    53401 ataaggatga gtagaaatga cgtttcgcat cgccaatttc catgtgcggc ttcattacac
    53461 atggctcatc ggattcggtc tcatcacctg gagtctcgcc gccaactact ttcacgacac
    53521 gcaggctgac ctccctgtca gctaccagtg ggcttccggt gcgatcgcca ccctcctcat
    53581 tctcgtctcg gtgttcctgc acgaattggc ccatgcggtc accgcgcggc gactcggcta
    53641 cgccgtcaaa accatcgaat tgcacattct cggaggctgg accatttttg agcgtgaact
    53701 gtccagccca agggtggaaa ttctcgtggc catcgcaggt ccaggttgtt cctttctcct
    53761 taccctgctg ctctatgcga tgaaagcaga cccgctggcg aactttctct acaaagtgaa
    53821 cctcacgctc ggcgcctaca atctcttcat tccgtgcctg ccgatggacg gcggccgaat
    53881 cttacgggcc gtcttatgga cccaaacaca ttcctttgcg gttgccactg agcgcaccgc
    53941 tcaaatgagt aaacaaatca gcagtggcat gatggccatc ggcatcctcg gcatcatctt
    54001 ccagtatcaa acgctgtggc ttgcgattat tggcggcatc ctccgcatga tcgccgaaac
    54061 cgcgtatcgc accgtgcaac agagcgaaca gctcacccga cccctgcaag aggtgatgat
    54121 tccatgggac cgggtggtga cgctggtcca cgatacgccc ctcctagaac tgcaagacca
    54181 gtttcttcgc tacgggtacc gtgcctaccc cactgccgag cacggacgga tcaccggatt
    54241 ggtgtattac aaggatgtac gcacagaccc caagtggctc gcgccgaacg gagcgaccat
    54301 caccccgttc attcgagcct tgtccccaga tgtgatcgta caccccactc ataccctgca
    54361 acgtgcgctc gatgcgatgc tcctgagcgg gtcggatcag ctgttggtgt ttgcgggaaa
    54421 cacgtgtgtc gggatggtga cccgctcgat gatcgcccgg gtccaagatg ccatcggcaa
    54481 tccagactcg ggccctacgc ttacgcggca gagggagggg tttccgattt aggactgttg
    54541 accgcaggag tgggaaactt cacttggacg tagggcgcgc tgctatccag cgtctttccc
    54601 ttgtaccact gcccatgggt ccgcatgtag aagcaccgct tgggcagatg caagacacgg
    54661 tctctcaaca ccaaccgttc ttcattcaga tgcaacgtga cattgccttg tacggacacg
    54721 acggaccgtg ggcgttgcac cagcggagaa gcatcctcca cgctttgtgc cgtctcggca
    54781 tgattgactc tgaaatagat ccagctgttg atattgtcac tgatactccg agcgagatcc
    54841 gcgccaatct cggccgacat ttgcgcaccc gattgctcgt agaagtgcag ccagacattg
    54901 gccccgccgg ccttattgaa aatttcctga atccccgggt acagcacgtt atgcccttca
    54961 tcgatatgca cacacaacgg cggatctaac tgacgaccgg acgcgaagag ccggccaacg
    55021 agactttgaa tcatagacac aaagacccgc ccaaaaatga gcgacgcgcg agggctcaac
    55081 aacgacccgg tatgacacac caagatcacc cgcttgcctt gttccagacg agtgatgaat
    55141 tcattggttc tcgccttccc gataatgcgg ccgatatttc caaaggtcag cgcgctcaac
    55201 atggtgcgaa gggacgacga caccttggca aaatgttccg cgatgccggg accatggcac
    55261 acccgatcga tcaagccaca cagctcctcc gtatcattca acacctttaa gacatctcga
    55321 aaattgatca attcggtgta gcccacccgt tgattaacat cattgaatga gatggtcggg
    55381 acttgccccc gactcgtcgc gagtttgatc aatcccgaga cgatgacctg cgaaacctcg
    55441 gaggcgatag agagatagta atcatccttc accttgatcc ctgagacgat atggctgacc
    55501 aactcatctt ccatataata atttgccaat ggatcgaggt agttggagta gtctgggaaa
    55561 atcggtgtca ccagcatgac ttcatccagc ctcccggcct ctgcggcgac ttgcacgact
    55621 tttgagaaga ggtcgatgtc ccctttggga tcgatcgcaa ccaccgattt gtggctccgg
    55681 atatcctgct cgacaatgga ctccatcagt cgtgttttcc caatgcgcgt cgtccccaaa
    55741 atccccacat gcccggaacg atcctcatcc cggatgacga gatacccctc tcgtcgctgg
    55801 gggtcttgca aacatgcgcc atagccgacc aagaccttcc cgtccacaat gggatctcga
    55861 tgtccccctc gattgcgggc ccgcaactgc tcaatgcctt gttccgacat ggtccctccc
    55921 catgctgtat taccggggtc agaagctcca ccaggtcagc acatcggcca tattcacgtt
    55981 caagtagccg cacccacttt attccccgac cattcacgaa tcagtaatac aacgatacgg
    56041 accctttacg gatcggacct cccaggagca ccatgaaaat ccctcttcat cactccagtg
    56101 acacacaaga tgtcgctgac gccgaccagg cgctggcgtc ccacactgtc gccatcggca
    56161 gaggcacaca ccgaagtctg cccaatcgcc ggaccggcta tacccaaaaa gctacgatcg
    56221 gaggacataa gctttatatc aggaccgggg aatacgagga cggcacgctg ggagaaatct
    56281 tcctcgacat gcacaaggag ggggccgcat ttcgcagtct catgaactgc tttgccatcg
    56341 cgatttcctt agggttgcaa tacggcgtcc cgctcgaaga attcgtcgag tccttcacgt
    56401 taatgcgctt cgagcccaac gggcctgtct cgggacacgc ccagatcaag atgtcctcgt
    56461 ccatcctcga ctacatcatg cgcgacctgg cgatcaacta cctcgaccga caggacctcg
    56521 ctcatgtcca ggtcacccat gacgaccttc gaggcgactc cgtcaaaccc tatgctaaat
    56581 ccacgagccg ccacacgccc caacccctct cacgcgcaga gactgcagcc ccgccgacca
    56641 cactcggcct cgaagaagcc agaataaagg ggtacgaagg ggacccgtgc ccagaatgtc
    56701 atcaattcac gctcgtccga cgaggcacgt gccgtacctg ttccacctgc gggagttcca
    56761 agggctgcag ctgaaggaac ccaggagcgt ctcttccctc aaacacatgg agcctctatg
    56821 tcagccccgc agcccatccc cccacacacc acttggctcc aacggttcaa acgcctggcc
    56881 cagttaacgg cctcgctcca tcctgaagat cctcgctttc caattgtcat gagactgctc
    56941 gagaagtgtg acggatacta ccggacggac aaccatgcct cgtttatcga aacaggtaaa
    57001 cgcatccgtt cgctgatgga caccccaccc atcggctaac gccactcccg aagcgaccgc
    57061 gccaccaacg tgcgctctgc actatttcgt ggatcacgcc ctctcacgct ctgaaaccaa
    57121 aggatccccc atgtctgaac aatacccgct ggccctcgat tttcgtactg cccgcaacgt
    57181 cagtgtccca ctcattgccg tgaactcgct cgacccagcc gccaccatgc gagccctggc
    57241 ctggtcattg gtcaatgtcg caagcgcctc acaagctgag accgacaacg aattcggcct
    57301 ccacgccttt cccatcctca agtgggacat tggccatggc ctgctccctc tcaacgagga
    57361 aggcaagaac tggcatgccc aatatgtcgg gccctcgaaa cagaatacga ccatcaaccc
    57421 tgcagaggcg ctccgcctcc tcgaaaaggt tcctgaacgc gcgctcactt tcattctgaa
    57481 cgggcaccgc tacctctcca acgatttctt catccagggc ctctggaatt gccgcgacct
    57541 ctataaggcc aacggcaaca cgatcgtcct cctgggcatc gggttcaaac tgcctccgga
    57601 attggccagc gacgtggtgc tcctgaacga accgctccct acggccgcac agttggaatc
    57661 catcatcaaa gagcagctgt cggccgcatc gctgcccgtc cccgatcccg ccacgctggg
    57721 caaagccgtc gatgccaccc taggccttgg cgcctttacc gccgaacaag aagttgctcg
    57781 ctccatgcaa gcgagcgggc tgaacatcga caagctgtgg gaccgcaaac gacaggtcat
    57841 cgatgcgaca ccagggctga gcgtctggcg aggcggaaac agctacgccc agatcggagg
    57901 ggtcgcgacc atcaagcgct ttctcaagca ggtgttggtc tccaaacggg ctccccgttg
    57961 catcgtgtgg ctcgacgaaa ttgaaaagag cctctccgga tcgaccggac aggcctcaga
    58021 cacctcagga gtctctcaag ccttactcgg gattctgctg agctacatgc aggaccacca
    58081 ggcatcagga ttactcctcg tcggccccaa cggaacctcg aagagtgcca tcgcgaaggc
    58141 cacgggagcc gaggcgaaca ttcccaccat cgctttagac atttccggtc tcaagtcctc
    58201 tctggtcggc tcgagtgaac aacagatgcg gcaagcgctg aatgtcatct ctgccatctc
    58261 tcaggacaag gcctgcttca ttgcaacgtc caacaatatc agtcagttac cgcccgaact
    58321 catccgtcga ttcggatacg gcacgtggta tgtggatctt ccaagtcagg atgagcgcga
    58381 ggcgatctgg accatctatc tcgccaaatt cggtctcgcc actgacgccg accgaccgag
    58441 tgatcacaac tggacaggcg cggaaattga gcgttgcgcg cgactcagct gggaactctc
    58501 cattccactg tccgaggcag ccaagtacat cgttcccact gccatcagtg ccaaggagag
    58561 cattaaagcc ctcgaaactc aggcgcatca aacctatctg agcgccaacc gcgacggcgt
    58621 ctttgaccag aaccgcgaca cgccgcatca cacacgccct cgcaccatta cgctcgcgca
    58681 atgagccggc cgtctcaact taaagcaaag gaacacacga tgccctgcta tctcgagcaa
    58741 cgagtctcgg tgcaattgaa tgtggccgat ttccccctcc tcatcgccgc gggcacctcc
    58801 ctcggatggg aacatgccat caatggcggg gtcctggtct ttaccactca acagaatcag
    58861 cgaatcacca ttgccaaagg acaggctacg ctggagcaac gacacgaaac cctgattacc
    58921 gaactaaagc aagcctatgc acaaaccatt attcaaacgg ccgctgaaca gttcgggtgg
    58981 acgttagagc gcgtcgaagg cgcagcgcag gaattgcgac tcactcgttt ctcctaaccc
    59041 gttggagcga cttccccatg cagaccgaca gcatacgcat cacgattgag acagacggca
    59101 ccatcaagct ggtcaccgac gagatcagcc tggccaacca cgccaccgcc gaagacgtta
    59161 tccaacagat cctcacactc tcaggcgggg aactagaacg cactcagcgc ttgacacaca
    59221 cgcatcacca tcatcacacc gacgaacatc ttcaccacta aacgaggtca tcatgctcaa
    59281 tgctgaatct gccttcggtt ccctctggaa gaacgctttc tgtctcatgc tcacgtatcg
    59341 aaagctcggc attcaacgcc ggatggacag tgccacggtg ctttccattg atacagaacc
    59401 tagccgcgtc cgagtgacca agtccatttt gtcgtgtccg gaatatatgg ccattacgcg
    59461 gctgtactcg cgcgtccgga ccaaactcga aaaactgact ctgccggccg gccttcgctc
    59521 gggcatgtac ctgatccccg tggcgctcgc cgccgaggtt gataccatta tcgccaacgc
    59581 cgaggaggaa ctccggcaac ttgtcgatgt ctttctcgca caatacgata cccggatcgc
    59641 cgaagaagcg gctcgcctca agtcagccta tcggaccagt gactaccctg atccggacac
    59701 ggtacgggcg gcgttcggcc tgacctactc ctacattact tttgatcttc ccggcacact
    59761 tgagaccatc agcgctacca tgctcaaacg agaagaacgc cggtcgatgg agaaggtcaa
    59821 caacatgctc gaggacgtga acacctacct tcgagaagcc atgcgatcgc tcgtcaacca
    59881 cctggtcgat cgattgagtc cgagtgacac gggcaagtcc aaaaccttca agagcagcac
    59941 gctcaccaat cttctggagt tcctgaacac ctttgaagcc cgcaaccttg gcaacgacgt
    60001 cgaactcgcg caactcgtca gctcggccaa aaacgtcctg agcggagtca actcgcaact
    60061 actgcgcagt gatcaatcga ttgccggcgt cgttcagact gggatgggta cgatcaagga
    60121 ccagctggat tcgcttcttg tgaaacgacc ggtacgcctc tacaacttcg acgaggccgc
    60181 ctgaccgtag gcacctctgc catcaccacg acacaccatc tttccggtac tgataacgct
    60241 catgtccatt actccagacg ccattgctcg cgcccgaaat atcaacctca ttgagtttct
    60301 catctctcgc ggccacacac cggtgtcgcg acgaccgcac catgctgatt ttcacgcgcc
    60361 ctatcgcgaa gacagaaatc cttcgttctc tgtgtcgcag cgacaggacg gcacctgggt
    60421 gtggtacgac ttcggacgca acgaacatga ggggtgcaag caccacggag acctcatcga
    60481 cctcgtccaa ctcctcgatc atgtgacgtt cgcagaagcc ctgcaccgga tcctgcacaa
    60541 caccgtcagg agtatggcga ctccgagcgg accctccctc gccccgccga ccgacaaaga
    60601 acgaattgct cacgccaaga agctctatta tgccgcacga gccaacatga ccccggaacg
    60661 tgaagaggag ctccggtcgt atttccagac gttagcactc ccctacaacc aacaccttgg
    60721 cgctgtatgg atgcgactgg gcgacgaagg cattccttac gtggcctttc cgttgcccac
    60781 acccaacatc catttcatgc agggactgat ggcgcgggcc ttgggcgagg ccccacccac
    60841 acttatgagg gtcgcccgtg gtctcaaagg tccctggatc ctgaaacgca gcaacgcgcc
    60901 gattctgatc acggaaagca tcgtggattg cctcgcaggc gatgaactgt tcggcccttc
    60961 gtttacactc tgtgcgctga atgggctgaa tacgggcgag cgcctccagg actatctcca
    61021 gcgactccct tcacgaatca tctatatcgc gctggataac gacgcaagct gtgccgccat
    61081 ggaacgtcca ccccacacca gccagcccaa aaaaggtcca cacgtccaga aggaactggt
    61141 ctcactgctg acgcacatgg gctatcacgt catggaagta ctcctgcatc acaacgccag
    61201 cgtgaaagat cttcacaagc tctggttgaa gcgccctcaa cgcgtgtcgc tgctcgacct
    61261 gacaaaaaca ggttttcacc actcacccgt acgctagcgt agccggtcac cttcgtcggc
    61321 ctcagccgtt cgccccggca cctggtcgca cgcaccgcac ggcgtccgct cctgaggaca
    61381 caggagccca atgaccaaac ctacaccagg tccatggatc ttagatcgta ggtgcgggga
    61441 catctctggc ccctcgccga cgagatggaa agatttgcca ggtactcaat gaagacggcg
    61501 tggcctacct tggccgacat cgtgtcgatg aggccctggc caatgccgcc ctcctctgca
    61561 atgcaccagg gatgttcgcc tggatcgaac gtgtatgcgc ttacagactc agcttccaga
    61621 cgatccaaga gggacggaag atcctcgcgc tggcgaggaa gcagtaatca cccgtgccgg
    61681 attcacccag cactcctggc ttggagtgct gtgcccagag ggttcctcaa aagcgccagc
    61741 ctattccttt cacacttgcc tgtgtctgtc gaaaaggagc ggtatgttca gcgaagactt
    61801 ttaccctaca cccggcgccg ttgcggcgaa gatgctccag aaaatcaacc gtaatgccgt
    61861 tcattttctt gagccaagcg ccggcaaagg cgatctcgcc aaggccattc tgggattcgg
    61921 ccggactggg tcccgctaca gttccgggtc acgccaccga gtcgacgtga tcgaactcca
    61981 ccctgatctc ttgaagattc tccaagccca tgaggagctg accgtcgtcg gctacgattg
    62041 gttgacctac gacggcgtct cctactacga cgccatcgtg atgaatcctc ccttcagcga
    62101 aggcgccctc catctgctcc gtgcttggga ctttcttcac aacggcgaaa ttgtctgcct
    62161 cctgaatcaa gaaactatcg ataatcccca tacggaagaa cgccgccggc tggccacgat
    62221 tatcgctgca catggctcgg tggagcccct tggtccctgc ttccgcaagg cggaacggcc
    62281 caccgacact ccagtcgtca ttgtctacct caagaagacg acagaggatg atcgcatcca
    62341 tctttggcat acaaccgacc gcgaacaatc tattaacgac gacatcggca cccctgaggc
    62401 gatccccgcg ctccgcgaca cgcttggtaa tatgcagcac tattacacac actccctgga
    62461 ggagatgttt aaggccttcg cccatatccg caaagccagt ctatttatgc aagcgctcgg
    62521 gaccaagctt ctccccggca gctcaatacg ggacgaagcc gatctcaaaa agatcctggg
    62581 catggcacaa accaacacca ccgccgcacg cgccgaattc gcacgggccc ttcgacgaag
    62641 cgcctggatg cacgtgttta aacaaatgga ctttcagaag tggctggata cgaagcagac
    62701 ccaggaactc ttacaagacc tggagcgaga cagtaccgtc ccctttacag caaaaaacat
    62761 caagggcacc ctcacgaaca tttttctaca gcgcaaacga ctcttcgaac gatctgtctg
    62821 gaacgtgttc gttgcgctca cccggcacta caagggcaat accaccgggg acatcggaag
    62881 tggtgacggc aggtccggct ggaaaaccaa cgactcgtac aaggtcaatt gcaaacttgt
    62941 gtttccctac ggctgtcgtt tttggaatga acgcttcgac ctgtggtcca cccgtgacgc
    63001 cggcgagctc tactccgacc tggatcgcgt gctcgctgtg cttgatgggg acaaattcga
    63061 ggagattatc accgtacggg atgcactaga atgctcattt cacaaccacg gggtgggcca
    63121 tcgccactgc gagagtacct tctttcgtat ccgctacttc aaaaaaggta cagtgcatct
    63181 cagatggaaa cgggatgacc tgttagccag cttcaacaca accgcagccg cagggcgtca
    63241 gtggatcggg accacctgcc acaatgatca ggactcccat gcctcatctc ctcattcagc
    63301 cagcaatacc cctcacgact ggccatcgcc tgcacataat tccccacgtc tcgccctaac
    63361 acattcagga gacacctagc caggatcggg tagtccctgg ggaaatcgat gcgtgagaga
    63421 tcgaagagcg cataaacgtg aagccactcg taagatctca gatcgtggaa acatatccgc
    63481 cggtgaacgc tcaactgcaa accatgatga ataaggagca taatgggtgc cgcctttata
    63541 cttgtccgcg aggtaaatct cagcgtggag gattcgaatg gcgtcgaacc cggagatcca
    63601 agaactagcg actgaaattg atgaatgtgc tgcgggtgca ttttctggcg gccggaccaa
    63661 caacctcact gatctagctt tgtcgatcgg tgccattgcg gccagtgcaa tcgccgccat
    63721 tcttgtcgca gacgggaaaa gtttccctct attcacagcg ttcgtagcgg cccttcctgc
    63781 cctcgccact gcgttgcagc aaacagtcga cttccgcgga cgagcaacgt ggtatttcat
    63841 taaatcggcg cagcttcaag aactgtctct caatctgaag tacggcggta ttgttgaaat
    63901 acgagaggcc gcgaggttgt ttggcgagat cgagagaagg atggaagaac gatgggtgga
    63961 ctttgtcaaa cccgtcatgc ccaaaaactc acatcacgct gcccaaccca accaacggac
    64021 aacttcagga gataagcagt aggcatcaag gtctggagaa gcttcgcatc gacgatgcaa
    64081 gcatcccgcg ggctgttctt tctcccccgg gaacgggcta atcaatggca ttcgagacct
    64141 gagcctatac gatggaagga cacgtcaagg aagtcgatgt gacagagtcg atctcgggtt
    64201 tgacctagat tcactgatcc agatctgcgg gtgttcggca ctgaacgacg ttgcagagag
    64261 cgaaaagaag ttgattgcgg tgccaccgat tctgcggtga agaatgaatg cccccatcgg
    64321 ttcgcgcagt tacttccgga tccaatcgat cccggtcaga ccatgcttga tcgcaaactt
    64381 cgtcaggtcg gcgtctgtat caatgccgaa tttatccctg aacatcgcct tgtggtgttc
    64441 aacggtcttt cgactcagcc ccaactgaaa cgcggtttct ttgatcgtct tgcctgaagc
    64501 caacaacgtt acgacttcac gctgccgctg cgacatcccc agggaggcga gctgatcctc
    64561 ttcgtcatca gaaccaggaa gagtgcctcc tggccaactt ccaagcaggc tcgtcacata
    64621 cgcgttgcct ctcattacct catcgatcgc atgacaaaac tcctgagcgc tgcaactctt
    64681 catcagatac ccgtttgccc ccacagataa cgctcgggtc atgtattcgg gttctctatg
    64741 catggtcacg acgacaatct taatgtcggg ccggatctcc cggacctttg gcacggcatc
    64801 aagacccgac atatccggca tcgagatatc aacaaacgcc aaatccgcag gaacccgctt
    64861 cacgagctcc agcaacccac gtcctgagga cgcttggccc accacggcaa attcgtagtc
    64921 tcgctgctta tggttgacac catataagat ggtggtcatt ccatctgcga ccaacgcatg
    64981 atcgtcagcg aaaaccacgc ttatcggttt gaccggagag ttcatcgacg aatccacact
    65041 agccgtcatg cttcacgctc cctgtaaggt gcccgagcca ctacgacagc acctcgccct
    65101 tttcctgact gaaccgtcaa ttccccttca accatatgga tacgctcttc catcgcgaca
    65161 atacccagtc ctttcgttcg acgcgattct gcttcggggc tgaaccccat tccatcatct
    65221 ttcacactca actgcaccca atcgtcttgt cgggccaacg ctatcatgac ctgctgtgca
    65281 ccagcgtgtt tctttacgtt catcaaggct tcctgggcaa ttcgatagag acaggtcgcg
    65341 acctcccgat ccaagacctc ggtaaactcc atagagatat tactgacaac cttcataccc
    65401 gaccaactgc catactcttt caccatcttc tttagcgcca ccgagagccc cagattgtcc
    65461 agaatcgacg gatgcagtcc gtgcgcaatc gttcgcaccc gatcgctgat tcctcgcagc
    65521 tcatcggtga cattcttgag gaccgcggcc tcagaactgt tgttctccaa caacaccaat
    65581 ttcccttcag ccttcaaaat tgcactcccc aggcgcgacc ctagatcatc gtgcaattcc
    65641 cgggcaaccc tacggcgctc gtcctcttgc gcggtcagca attgggaatg gagggcacgc
    65701 aacgcttctt ggctacgacg tagagcctcc gctccctctt gaatcacact ttcggcacgt
    65761 ttccgctccg acacgtcgcg cacgatcgca cacacatacc cctccccatc gtgttccaag
    65821 catcgcatcg acacttcgat agggaagacc gctccagact ttctccgaaa agtagtcgca
    65881 tactgaacta cttcgccctc cttcagccgt gtcagatcgt taagaatgtc gattcggctc
    65941 tgcaacggcg caatatccgg caccgacaaa ttcaagagct cttcacgtga atagcccaac
    66001 aactcggctg ctcgcacatt gacatacatg atgcgacgat caggccccac ccaaagaatg
    66061 gcatccgccg catgatccgt cgcaaactgc gtaaaccgca gctcggcttc tgccaaccgc
    66121 cgctcagtca catcggtcaa caaaccgtgg aacagcaccg ttccatcggc ggtaggaacc
    66181 tccggcaggg aatctccgcg taaccatttc acccgaccat cggggagtcg caatcgaaac
    66241 tcatgcgccc accgcaatcc acgccgaatt gcatcttcaa tggacgccat gatccctggc
    66301 atatcttctg gtaggaccag tttccagacc gcgttatagt ccgaaacaat gaggtccgcc
    66361 ggatacccca ccatgtccac ggccccacga ctgatgtaac taaaccgctg gcacccatct
    66421 cgcgcaatga catattgata caccgcacca ggcaccgcat ccgcgatgcg tcgcatctta
    66481 agatcgctct gacgtaactc ttgcacctcc tcaaaatagc tcttctcttg atccacaacg
    66541 aacgactcat tcagctcgag aagtcggcga tagatccctt gggcaaacgc gaagccagcc
    66601 tccttcgaat atggcgtccc ttcaccacgc ttggcccgag cactccacca ctggtcatct
    66661 ccagccgaac acgccagaga aatattcccc gacaccattt gaaacaggga cttagttttc
    66721 gcatcgagcg gcacattggc atagaggaag acacagtaac tttctccaga aggcaacggt
    66781 ccccccaggc caacaatctg tttcacctca tgcgtccgca gaagctctgt tctctttccg
    66841 agtttcggtg aaacaggaac ttcatgcaca ctaatttcaa acaattgatt gtgtgaatcc
    66901 gtaacgacgc aatcgctccc caaatagttc actggcctgt tcaggcccaa ctctttccac
    66961 gattgcgtgt accacgctgc ttcttctgaa tcgaactcct cctgaaggtg gatgcaatgt
    67021 cgtccgaacg gaggcgttcc gactgtccat tccacagagt ctccagccac ccccatcaat
    67081 gcgagaacgc gggtttgagc agaagcgtgc gccccatcag ccgtctgtac ccatcgctct
    67141 tgacggtgga tcgggatctc accaagattt cgcgtttgaa acacctccac aagagacagt
    67201 tgcgagcgac gaagttcgtg agaataaaac gaacgcacca gacactccgc gatcgactgc
    67261 gagatctctt ctgacgatct tcccgctgtc tctatacgtc gcaacttcga cccaagacgg
    67321 cacacatcgc cgactgagaa ttcttggaga tcaaacgtcg agtcttggcc tttgatgcct
    67381 aatgacatgc cgacctgtac cttcccgcct accgcctcac accgcagttg gctgatgcac
    67441 gatgcgattt cccgtcagag aatgccggtg gcacttgaga gagtcatccg gcaatacgta
    67501 tttccacgct atcagacttc ataattcaac cgtcacagta tggcaacaat cgttcgatga
    67561 ctcaatactc aacgtccact tcaagcgaat ctcttaccgc agaacactgc tccatttcca
    67621 gttgttccaa atagaaacat ctttgaggtc cggtccattc tgtgtgatcg ctgtcccacc
    67681 taatatccca aaaggcactc tgttgctatc cggcaataca tagcagaagc ggttcagact
    67741 cgttcaccta atccaggagg aaagcatgtc accgattatc cgtcgcaccc tctgttcagc
    67801 cggcagagcc ttcgtcattg ttgttgtggg tattgccacc ggatcgatct ttggctgtgc
    67861 tggccacatg acgctaagcc atgaggtcaa tctcaccagc agtaacgcca tctttctgac
    67921 cccgtcggag aagaagacta tctatctcca gactcgaaat accagcgaca accctaatat
    67981 caactttggg gaactcccca acaaaatcaa agccaagggg tacgcaatcg ttgataaccc
    68041 cgacaacgct cagttcatcc ttcaaagcca ggtggtgctc tgcaataaac tgaagccggg
    68101 gcagaccgtc gatgccttga tcgccggggg attcggagga gccatcgggg tcgcagccgg
    68161 atctgccgcc gcgctcagcg gggcctcact cagccatatt ccgatactcg gggcggtggg
    68221 agccgtcgga ggatttgccg cgagcaaact ctcagaagat tctatcgtcg cctgcgtggt
    68281 cgacgcgatg gtacaggagc gcaccaagga agaaattcaa caatccatca cgacgaacag
    68341 catgcccggc ccaggggttc cacagtcaca agccggggcc ttcggctttc tgaatcagca
    68401 gagtgtctcc ccgcctcagg cagggcaggt cactcaacaa atcggcgaga tccggaaagg
    68461 cactcgccgc ttccatcaag cgagaattgt ggcgtcggat caaaagatgt ggctgtccgt
    68521 tccggaagcc agcaagattc tcagcgacaa actggaggat tcattggcag gcctcttctg
    68581 atgccccgat ttgaaaacaa ggactgctca ctctcagcga ggcgcagaca gcgcctcgct
    68641 caatgatcga tccgctcaga tctggagacc atgctgtttt tcaccatttg aatccttaag
    68701 gatgacccca ttcttgctta ctggacgagt ctcctcacct ggaccattat gtggaccgtc
    68761 atcctgtcca aaaggatccc gtttcccagg acatcccgat ctccatggcg ccccatcgct
    68821 cactggtagt ccgccaacag tccctcgctc accggcaccg attgaaggca cgaaagactg
    68881 gagttactta accttgtcgg atgtggccca agggagcccc agccgggcag catcaccccg
    68941 aatgagactg aggagataac ttccttggtt gatcgtctgt ccagataatc gacgttcctt
    69001 cacttccccg attcgtcgcc agatcatcgg atggggaacg ctggtgatcg cacgcttcca
    69061 ccatttctcc cataccttgg ggtccttccg ctttccggag atctctagca cgacgctcag
    69121 gagccgcgca ttcgaggctt ctcgttgccc atccttctcc tgattgctct catcagattg
    69181 ctcgatgagg aggaacggat ccagctctcg tccgatctgt tgcaactcgc ggtgattggc
    69241 tcccaccatc tcttcacgga actcccgcgt cttcttgtcc gtcgcccacc aggccgggcc
    69301 cgcctcccaa ataatctcaa actccttacc ctccggcgtt cgaatcggca catactccca
    69361 gcgtgaaata aactcctttt ccagcagctc gcgatgtgcg tcgtctaatt gctgttttac
    69421 caatgataga gcgctatgcc gcttgatctg ccaattccgc accagctcgg aatacttttg
    69481 tgaggccgtt ccacgcccaa ggtaaaacag gttgtgcagt aactgatgca acagctttgt
    69541 aatcgatcca tggaggtccc gaaagagttc atgatcgatg acgatacaat tgcccgacct
    69601 caggctcatc cagtaccacg tggcaagctc gacctcatac ttatccgcca catagccttc
    69661 cgcattgact tgccccggta acaccaccgt ttgaaaaata tgaaagacga ttcccttgtt
    69721 cgaacgagga cgtttctcgc ttccccccga ttcgccttct tgtcgccaat ctcgcccggc
    69781 gccgatcatc gtgccggcca tacgcagaaa gaaccgatcc atttcctggt acgaggcacc
    69841 gccaaggcct ttcccgctcg cctccaacaa ttcacgacct gaaatcttta ctaactgatt
    69901 aaatggctct cccctactca gcgcggcttt cgtccatcgt tcaaaccctc gaaagaactc
    69961 cagatcaatc gaattcggaa gccctaaatc gccgctgccg tagaccacta gggagacttt
    70021 acataaccga ctgttacgcc acacatctcg ataaatctgt ttttggtacg aggctcgttc
    70081 cgcacctgtt gggcggcctt tcgcttccca cagtggaatt gatcctaaga tatgctcgct
    70141 gaggaggtgg tcgggggtgg tgaccgatag agagccgtga tccgtggtgg ttccagggaa
    70201 gatcagttca gcgggaatcc cgtcagagga accagtcccc tcgtccgatt ccgaaggcgg
    70261 tgtgaaatca gcgtccatca gcgtaacgtt ctttattaat tctttcgtaa gtttctaacg
    70321 tagagacaca gtttatcagt cgcttaattc ttgtttttcg ttgcgcaatc aatgctttat
    70381 aaaaactgtg tcgttcatcg aagtagcgtg acttcattca acttacgtcg gctttcattc
    70441 aacttacgtg aaaatggagc cggtttcttc attcaacttg cgagaccttc atcgaactaa
    70501 cgcgaaatct tcactcaact agcgataggg ctgtggataa ctaggatcgc gcaccacatc
    70561 gatattcgcc cccgatccgg taatacagta aatcttcaaa aagactggaa tatttgaagg
    70621 tggaagaaaa ccttcccaac ggggatgaga acgccgcccg tccgttcgta agttgaatga
    70681 aacttttcac cgaagatccc aggctaggtc aacttgtcac gaaactcctg gatgcgctcc
    70741 gacgcctgtt ttaaggcttc ttcaatcatt gggatttcat gcggcttgtt ggaatgaaag
    70801 agaatggtca gcatgaaccc acggtctccc ccccgcccac gctccacgaa ccgcacggct
    70861 tgggcacctt cattaattcg tggtttgcga tcgatttcct gtcggatccg atctcgagtc
    70921 cacgattctt ttgaggcacg gcgagcgatg gcgtgaaggc gtggctcgtc atgggccaaa
    70981 gggaggagcg cctctgcatg cgtcgccgtc atgcggctct caggtccgaa gaacacgttt
    71041 aagatgctct ctggcagctt caggatgctg agcgccctag agatacgtga tttgtgacag
    71101 cccaggcgtt cagctaactc ggactgctga aaaccatacc gttcgatcag ggcttgatag
    71161 gccctggcct cctcgagggc agacagatcc ttccggacaa cgttgtcgac cagcgcctca
    71221 gccgccgcgc ggtcccggtc ctttcgacga acgagagccg gaatcgaggt ctttccgagc
    71281 aacgcacagg ctcgccatcg ccgctcgccg gccaccactt cgtacttccc atcctctacc
    71341 ggacggagct gaatcggcgt gagcacgcca taggcacgaa tcgattccaa tagctcctgg
    71401 tcagccgcag gatcgacctc agatcgtggt tggtcaggac tcggtaccag ccgatcgagc
    71461 gggacttcga cgacctcttc gaggcgacgc aggtgatctg cctcttgggc gattatggga
    71521 accgctttgg ggccgacagg cgaagccgta gcttcagcac ccaacgggat agagacggac
    71581 gcgctgccat caacagccga gtcgaaggtt gtcgatggta tttggctcgg gggtgtcgtg
    71641 tgttctggtc cgggctgtcc accttgtgta tccaaaccga ccagggcaaa cgggcgaatg
    71701 cgacgagcat cacgatcaaa ggaggcttcg agatcagcga gatgtgagga cttctggcgt
    71761 tcaggtttgg cctctttgct tttcgagaga ggcggcttct cgtctttgcc tttggagact
    71821 tgtttgctcg tcatgattca cctgccccga cctcgaccgg ttcatccatg tcgtcaggct
    71881 ccgccaatgc acctaaaccc atgcgagcca agacctcgcg gcctagatcc atgaactcgc
    71941 gtgcagcatt gctctgccga tctaattgaa agatcgtctt ctttagcgat tccgattctt
    72001 ggatcttggc ggccagggga attgcggtct taaacacaag atctccgaaa cgacgttcga
    72061 tcacgccccg catggacttg cagacgtttt ttcgtccatc atgttgcgtg acgaggatcc
    72121 ccaaacactt cagggcagga ttagcgctat ccaccacatc gcgcaccagc tccatgaggt
    72181 cttcgtatcc cgtgatgctg tattttgagc cagacatcat aggggtcaaa tagtagtccg
    72241 aagcgactag ggcgttgatc gtgaggatcg acagggtcgg cgggcagtcg atgaggacga
    72301 tatcttcatc cccaaaggcc atctgctcca ggcgacgctt caggacgatg gatggcatcg
    72361 tcggataaac cctgggaagt tctcggtccg ctttggtcaa ggacacatgg ccatagatca
    72421 ggcggacttc cttctctatg gtgtcatacc agggtgcggt catgtcctca tcgcgtttgt
    72481 tatccatcat cagtgtggcg gcagttactc tggcttcgta tggacttacc tttccaagtg
    72541 tcgaagatgc atttgcttgt gggtcaatat cgaccacgca aacgggatga ccaaggcgag
    72601 agagcgcagc tgcaaggttc accaccgttg tggtttttcc caccccgcct ttttgattga
    72661 taacggccac tacattcggc atcgatacct cctcggccta ctatgtatag gccatatacc
    72721 agagcgtagg aaaaaccaac ctactcagga catgtaattt gccacggcgt tttgtctaac
    72781 atgtgttgga ttagcttgta gacattgggc cgcaattgac tgtttcatta ctcctcatgg
    72841 tggggcctgt caactaatta atataataac tatatctcga cgcgtcactg taaaatgttt
    72901 tgtggttgcg cgggcgcaac tcacgaaggg atatggaggg gtctttcccg atattgcgta
    72961 tgcgaataat gctcgtaaat atactgctaa agattgttag gcaaattgaa tgtaagtgaa
    73021 tgattataac tatgtttgtt aggttcaggc gacgatggtg aaatatgttt tgtctgtgag
    73081 gtcgatacta gtggaatatt tacgagttgc gcgggcgcaa ctcgtatcgt ctgagaagtt
    73141 cctaggttgg tgaggacagg gtggggtact tggatcggag gcgtccgtac atgcgctgtt
    73201 tcagcaaata gaagagtagg ccttgttgaa aggtcgtttt atcgggattc tggccgatat
    73261 catgaaacct ggcacgaacc tcctcttcga tcatttttcg ctcatgggcc tgtagtgact
    73321 cgtatacgac ctgcaaatca gcccgatctt ccgcgctcaa ttgggcaagc aggttctcaa
    73381 tcgaccggcc atctgggggg gccgttttag accggcggcg cactggcaca cgcttcgcgc
    73441 taagtcccaa gactttttga taggagcagg ggtggatctc atagagaccg gggtgcttcc
    73501 cgcctcccgg atggacaaca ttcacgatat ccacgtcagg atcggccaag cggtggagtg
    73561 cttggtgaat ggtggagcga ctcaagcccg tcctcgcttg gagacgatca agggtcattg
    73621 ccacaggatt gcgtcgtcgt ccgaaggcct ctcgaacgag ctgcaagtaa accacttgtg
    73681 ttggagccga gagcgccgtc atccctccat ctaagaggaa gtggagaaga ccagtgtagg
    73741 cgaggggtga ccacacggat tcgctgagga gggcagacga aaaaggcgcg gattcagccc
    73801 tgccagtagc gggattctga tcaggaatat tcatctaaac cgttgatatc catgcatgat
    73861 tgacgctatt tgtgtaatcg tgtcatttgt ccatatataa gtctgtatag tctgctaatg
    73921 gacgaaagtc aagtgccgtc ctactacttc ataaaagaga acctttgacg aagtagtctg
    73981 attggcttat tctgatggct accatcccgg accagaagga gatccatacc gtggacgagt
    74041 ccgactcgac gtctgatggt gagtcgccaa tgaccaggct gccgcgtgag agtggggagg
    74101 ggattaagaa cccgctgagg cttcagcctg gtcgagatgg cgatcacgtg catcttcaag
    74161 cgacgttcta tcattacctg ctcttttgtc aggcaaacaa agcggcgagt acacaccgga
    74221 tctaccaatc caccctttgg gccttctatc gctggtggag gcaagaacgg ccatttgaga
    74281 ctctcggtcc cacactgctc catgcctaca agatttggtt gtgtgagagt cgggatcggg
    74341 gacaggaacg attgcaggcg cggtcgatca acaactatct cgcggcagtc cgggccttct
    74401 gtcgatggat tcacgcccga tacccgctca gttgggatcc cggccgagag attccggacg
    74461 agcgtgtcga taacaagacc tatcagcgac ttccactcag cccctcacaa gtcacgacat
    74521 taattggcag cttcgatgac tccctgatcg ggcgccggga cgccgccatg acgtacctca
    74581 tggcgaaaac tggcttgcgc gcgatccaga tccagcgggc ggattgcggg gacctcgagt
    74641 gcctagagat ccaggtctac cagagagtgg atggggcaag ggaagccagg ggtccggcgg
    74701 tcgcacgatc gcaatggatc ttgcggactc agggaaaggg acagcagacg aaagaaagtt
    74761 ttgtgaattt gatgccagag gtctatggcc ggcttcaacg atatctagag gcacgcggtc
    74821 cggtgttacc acgcgagccc ttgtttgcca ggcaccatga taagctcacc tcgcatctcg
    74881 cgcaggtacg gtcaaagccg ggcggagggg aggcttcaac gttggcgcca aatgagcggt
    74941 tacgactaac gacccgcgcc atccaacttc gaattgcggg agccatgacc cgctgcggcc
    75001 tgcgggatgt cacgcgacct ggcgaggcgt catcgttgga aagtcgccgg cgtcggcaac
    75061 gaagggtgac gccacatagt ctccgccata cggctgcgac ggaggccgct cgaactgaat
    75121 cgccattcaa agttcaagct atgttggatc acaaggacat ccgcaccact caaaagtatt
    75181 ttcacgcgat tgatcggctg gaggaagggg cggagtatgc gatcacacgc tactaaagag
    75241 tctagccatg acactctcaa cttcggtgcg gatcaatatg gagtttttga cacgcgttgg
    75301 gctctcacag actgcgatca cataaataca taacgtctgc cgtgaataaa catttcatcc
    75361 acgctggagt ccaatagact ttaaggtatc ccaagtgggc gggcgttccc taggttcccg
    75421 acattccagg cgtggagact cggacaccaa atcctaccac gaccatcctc gacaagcgcg
    75481 tcccacgacc tggaagagag gcggagtatg aagcaccggc cgtcctcctg cccgggctgc
    75541 gggcgctcta ccgcaagcgc aatcactgct ccgacgcaga ggttgatggc gttgatgaag
    75601 cgattctccg actgttgagt tcggggaatc gttcctgaat actcgacgac ggcgcgaaaa
    75661 ctcccaaacg gagtcgaggt ttaaacggct tctcgcggag ggtggcttgg tcgtacatcg
    75721 agcaaccagg caccatcccc taaatttcca atggaagcct tgactcgaca gacgagagac
    75781 gctaaacttc aactagttgt agactagatc aattggaggc aggcacatgg atactatcgg
    75841 agcatttgag gcgaaaacgc atttatctgc cctgcttgaa cgggtgagta agggagagac
    75901 gattaccatc acgaggcacg gcgtgcctgc tgcgaagttg gttcccgtac agggagatgt
    75961 cgggcgcctg cctcatgcgg ctcttctcga gggcatgcgc gagttgcgca agcgggtgaa
    76021 gccaggggcg atgcccgtca aagacatgat cgaggagggc cgtcggcgtt gaggcgcgtg
    76081 gtgatcgatg cctcggtggc gctggcatgg tgcttcccgg atgagcaaag tccttatgcc
    76141 gacaaagtcc tccaggagct ggacgggtgt tctgttctcg tgccgtctgt atgggcgctc
    76201 gagatcacca acgggctgct tgtcggtgaa cgacgccgac gcatcggcca atccgacatt
    76261 ttacggttca tcgatctctt ggacgcgttg tcaccacagg aagtctcagt cccgataaac
    76321 gcgcattttc acgctcttct gccgcttgga agaggatacg gcctttccgc gtatgatgcg
    76381 gcctatcttc atacagcgat aaggactgaa gctgaactgg ccacggccga cgacgcgctg
    76441 gagaaagcgg cgcgtacggc tggggttccg atcatgtgca catcacatct ccgtaaaccc
    76501 agacgataac ttgaaatgcc tagttgatcg ggtaagacgt aggaggatgg cctgatgttg
    76561 tgttgtgact tctgttcggc ttcaatcccc accaccaaat atcggaccca cccattcgga
    76621 ctgaccgact caagtcaggt ctttggcgat acgccgattt atactattca agggctcgac
    76681 catgtctcca ctgaggattt acatccgtcg catggcacgg gtgccatgat gagtcctgaa
    76741 tggctcgcct gtgacgactg cgccatcttc attgacgcga gggatagaga agggcttgtg
    76801 caatactgtg tgatgcgcct ctcagcacaa cccaacctgg aatcaattga catcgcgaat
    76861 gagctgattc ccatgtatcg aaagctgttc accgctttct tcctttacct tgaatcaaaa
    76921 gaaccgtctg ccgcgtcccg atcagacctt acctgacgct gcctgataca tgcactttcc
    76981 ccccttcatc ccatacctcg gccgtgacca aaaccgatgc tgtactcatg aacgagcgaa
    77041 tcccacctct gtaacgctac tctcgaggtg gattttttcc cgcaccgtct ggctgccgca
    77101 ttatgacgga gcgcatcttt aatgcgaccg tggcgctcgt agagaccggc taggcagcag
    77161 tgcgcccgct caaactcacc gtcatgcccg agcgcctact cataggcatg catcgccgcc
    77221 tggtccttgc cttggtcttc aagtaccaca cctaaattga agatgcgacg gctccatctg
    77281 gatcggagtg aatcggcgcg cgatggctcg cctccgcctc tgcctgtttt tccgcatcat
    77341 gacacagccg tccgagattc acacgtgctt cggcgaatga cagatccagc ggaatggctt
    77401 cttgatacgt ctgacaggcc gttctggcac atgtccttca agctccacgc cacgattcca
    77461 tcagaccaac gccgatcttt tgtctagccg actggttcgg cctctcgatg ggagccgggc
    77521 cgttctggcc ggcagctgca cctcgaaatt cagcacatag tgaccagagt ccggttgcca
    77581 tcggtctcgg ccatcccaca cgatcactcc acgccttgac gcatagactt tgacactagc
    77641 catggagcga gtctccaaac tgccgctgca ggaaagcgag cacggtccgt atccgtcgca
    77701 acgaaacccg acgtccttca gaccattcat agtccgtaac cacctgctgc tcgaacgaat
    77761 cggatggcat atgtgttttg aggggcgaca cggtgagaag ggcggcgcgc aggtaggtcc
    77821 gcaattcggc gttgctaatg tgcaaaatac tacacagcgc ggaggaagcg tagtacccgt
    77881 catattctga aatatcttgc tgaccctccg catggaatgc agtcacaagg attaaccagt
    77941 ggggattcca tgcgattgat cacgacgaag gaactgagaa acatgcacat tgccacacgg
    78001 catcatcatc tcatcaacag ccggctggcg atgttgcgct atgccgacga atatgggttc
    78061 aagggcgccg cccgacggtt tagcttgaat cgtaagacgg tgaggacctg gcaccgacgc
    78121 tgggtagtca gtggaccggc ggggttggtg ccacgccatc cgcgcacacg cgccgcaagc
    78181 tgcactttgc cgcgatgcgg acccggttct tgctggatcg ggtgcatcgc atctgcgtag
    78241 cggccgcgac atcccccgaa tctcccggaa cctcggctat ccgcccattc ggcgcaccgg
    78301 cccgcgacac cctgaacaga aggggagcat cagtacaacc acgaccgctt ctctatggcc
    78361 ttccagggcc tgacaccagc gaagaaattg gcgacatttc tctcgccacc ggcattatca
    78421 tcaccaacca cgccatgcgg acatacaggg gccgcggtgt gaccagccat tgcaacccag
    78481 gaccctcgcc ttgaaagata ccctctcatt cccatcttgt tgtctactga atatcgtcca
    78541 gatagcgttc cgctccaaat cgtatgcggt tgacctggtc aatgaggact tgggtggtct
    78601 gaatggattc atgccgcatg gtcttcttga gctcagatgg gctcgcccct ttccgatagg
    78661 ccaaggctcc gcctgacagc cggagtgaca gggcggtgac atggggctca gcaaggccag
    78721 cttccctaaa agcggccttt agctgcttgc gcattccccg tgtcgtcatg cgttcccctc
    78781 g
//
LOCUS       DAOQDS010000008        74573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_108_length_74573_cov_21.661129, whole genome shotgun sequence.
ACCESSION   DAOQDS010000008 DAOQDS010000000
VERSION     DAOQDS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 74573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 74573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74573
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108_length_74573_cov_21.661129"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(456..1715)
                     /locus_tag="PK589_03000"
     CDS             complement(456..1715)
                     /locus_tag="PK589_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ18592.1"
                     /translation="MAEIAISARHVSKEYVIAEINHDTLRDRIAHGCRSLLGRRAAKS
                     VPPKSFQALNDVSFNVMEGEVLGVIGHNGAGKSTLLKILSRITEPTGGEVDLYGRVTS
                     LLEVGTGFHAELTGRENVYLNAAMLGMRKVDINKRFEAIVEFSGTQQFIDTPVKRYSS
                     GMYVRLAFSVAAHLDPEILIVDEVLSVGDGAFQKKCLGKMDEVRRDGRTVILVSHDLP
                     VVASLCQRAILLRHGRVVGDGQPQQIIEQYMSEAFTGTGVSLADRTDRYTKGELTVQA
                     ITFLDDKLNPLQPALSGQHLVIRVQYKCHVRKAFKDMRVMVVINRDERTSCIFSTDLV
                     SHTPLDLEGSGYVDFHVPHLPLCGGRYFLHAAIENNTMTQDWVQYAAELQVLDGDYYG
                     TGRMYPHDGWRGKGMFVDHSWRFERTS"
     gene            complement(1715..2599)
                     /locus_tag="PK589_03005"
     CDS             complement(1715..2599)
                     /locus_tag="PK589_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ18593.1"
                     /translation="MNESELQQASPLTAGTLAPAVRTPTVKIRPSRGLFHLDLQSVWQ
                     YRELLLFLIWRDTKVRYKQAVIGAGWAVFQPLISMLLFTVLFSYLAKLPSDGVPYPLF
                     AYAGLLPWNFIAQATSRSGTSLVGESHLISKVYFPRLIIPLAAASAPAVDLLCALLMM
                     IPLMLWFGVTPGWQLLFFPVFVGIALLAALAVSFWFSALHVKFRDVGHIIPFFVQFWM
                     FASPVVYPASLIPEKWRTLYSLNPAVGVVEGFRWSLLGQRPPSLETILPSVGIVLVLF
                     VSGIVYFKRMERTFADVI"
     gene            complement(2616..3431)
                     /locus_tag="PK589_03010"
     CDS             complement(2616..3431)
                     /locus_tag="PK589_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HNJ18594.1"
                     /translation="MSSVAVVVPGYNRAEFTEDEEISFRHLEHYLGRYDKFLVVPQSL
                     AIERPGFHIQRFPDSYFGSAIANARLMLSPTFYGAFQSYRYVLIYQLDALVFSDRLME
                     WCASDWDYVGAPWLKCADSPWVGASRVGNGGFSLRKVSSFLRVLSSDAYWVDPEVYWQ
                     RITTGQSWYVKSVNLPRKWYKQIKRFNNVKRELERWHLRPDGTKNEDHFWADEAVRYD
                     AQFKVAPFHVGLDFAFEVVPRHCFELNQNRLPFGCHAWPRYDRSFWEPYLIKP"
     gene            complement(3474..4205)
                     /locus_tag="PK589_03015"
     CDS             complement(3474..4205)
                     /locus_tag="PK589_03015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNJ18595.1"
                     /translation="MSLQSFLSTSVRKVLGSLTHVVTPSAVLALTFDDGPDPENTPKV
                     LALLEKYQARGTFFVVGQAAAVQPALVQRIAAGGHAIGIHSWDHRAFPGLTSRERRRQ
                     IRECERALRPYVSPLFRPPYGEQTVMSRLEAFMMGYEVVGWNLNSGDWYESQSSVMGK
                     YLLEMVSPGGIVLFHDTLYDKGTPAHGTDSEHTSWPDRQAMLDALETMLDRLSGSYRF
                     VTVPELLQSGRPRRSFWFKPSLATA"
     gene            complement(4222..5097)
                     /locus_tag="PK589_03020"
     CDS             complement(4222..5097)
                     /locus_tag="PK589_03020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ18596.1"
                     /translation="MTLSIIIPCRNAARTIEETLQALADQKWPAAWEVIVADNGSTDN
                     LARVVAKYTLRIPHLRLVDASARRGASYARNIGVNAATGEYILFCDADDVPAAGWAIA
                     MEKAVRMHGFVASRLEFEKLNSPVVRSARGCTQIVGLQQFRFLPFPHAGSGTLGITRA
                     LHDAVAGFDETIPICEDIDYCMRVQQQGIKLEFVPDAVLHCRLRASKKGVYAQASQYA
                     EYEVYLYKKYGAGHLWEWWRWRKYIQAWRNLLGRMSELLRTPEGKTLFAWRLGRLMGL
                     LKGSLRFWAPPVMVE"
     gene            complement(5133..>5507)
                     /locus_tag="PK589_03025"
     CDS             complement(5133..>5507)
                     /locus_tag="PK589_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18597.1"
                     /translation="AVREAPWLLQEAAVVEWLKRIASTLAGSSSHPVAAVKKFWKEIT
                     NAVAEAETIYTGSLRRLFGELLEAAAISMKRGSPRRAPLVAVSALLNDPSSWMQPARL
                     YSLYRAWTRESLATAEHAKVSP"
     assembly_gap    5508..5607
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5608..6559)
                     /locus_tag="PK589_03030"
     CDS             complement(<5608..6559)
                     /locus_tag="PK589_03030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ18598.1"
                     /translation="MNNESLSRQEGGVNAMPCPSPERPLVSVVIPTYKRAHLLRKAII
                     SALAQEQAGELFEMEIIVVDDCSPDETAKVVAEFPRVQYLRLDQNRGASGARNAGIKR
                     AKGKYIALLDDDDEFLTHKLMVQVPILEAHPEIGVLYGQSVVTGGETPLLLWPQWGPS
                     GNVFEEFVTTTDDFLHPPTWLVRRELFEQAGFFDESKAGMEHYDMALRIAALTPWMFL
                     SGGPVARGRYSQQGLWYSTIVNGTNEQQLPKIIEAALLRLPATADADRVRRKARAAVC
                     ASIAHQRWWAGGGLEPTKAHLLKAVREAPWLLQEAAVVEWL"
     gene            complement(6739..7839)
                     /locus_tag="PK589_03035"
     CDS             complement(6739..7839)
                     /locus_tag="PK589_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNJ18599.1"
                     /translation="MKKLRVAVLGTGAFAEACHVPGLQSHPGAEVVVLCGRNSARTRA
                     MADRLGVPEISLDYEQVCARNDIDAVTIATPNVAHALQAKAAFSAGKHVFCEKPLGMN
                     VQEVIEMVRAAERSHAIHQVAFTYRYLYGVQELKCRLLKGDIGEPYHVRVQYASWEGV
                     KPDSKIGFREKRDCAGAGILYDVGSHLFDLVGFILGPIEAVAGSTLLIPRERIDHRTG
                     VLGQVETDDIASAWFVCRDGIQGHLFASRATPNSGDKAYIEVVGREGALRASLSRGSV
                     DRLRVSRSTHPGWEQVPLPAAASDGKPHCLGAMMRSFVDACLRKRLNHDVDASFYDGL
                     AVQRTIEAVQESACRPQWIPLESGVRCNVPGE"
     gene            complement(8048..8701)
                     /locus_tag="PK589_03040"
     CDS             complement(8048..8701)
                     /locus_tag="PK589_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ18600.1"
                     /translation="MNKIRVLLAEDHTLVRAGFRALLEKLEGIQVIGEVSDGRDALRV
                     AKELDPEVVLMDIAMSGMNGLEATSRMRQECPRTKVLMLTMYTNEEYLKEALRAGASG
                     YLLKDADRPELELAIKTVCRGETYLTPAMAKFTLDAYCRQDDVPMSPLSKLTGRQREI
                     LQLIAEGCSTKQIAHRLDLSVKTVETHRAQLMERLEIHDVPGLVRLAIRTGLVRSDT"
     gene            complement(8698..10218)
                     /locus_tag="PK589_03045"
     CDS             complement(8698..10218)
                     /locus_tag="PK589_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ18601.1"
                     /translation="MIEHHDTGLSRVLVISTRPDGIITSFNSAAERLLGVGADAVVGK
                     VTPAAFHDPDELRRRREALAAGLGKPIQDSFGVLVARVAIGRMTEDEWTYLDRSGYRF
                     PVFLSVSAIPDEAGHVLGYCFVAKDRRGQLQAEALLRRQAKLLDLANDAILVRDLQAD
                     TITYWNEGAVRLYGWTAEEALGAYIHDFLSTTFPLPLEEIKQQFLEQGLWRGELVHKT
                     RAGREITVSSRWTLLRDAAGMPSGSLELNTDITEQKRAQKALKDAHEELELRVMERTT
                     DLRDANERLRILSRRLMEIQELERRAIARDLHDEIGQALTAIKLNLRELRTHPNCEPV
                     EDQIADSLEILAQVLQRVRSLALDLRPSLLDELGLVPALRWYVGRQAERAGWQLTFSA
                     DGLTSRPPAEIEIACFRLAQEALTNVVRHSQAKKVEVLLERSSQALTMVIRDNGIGFD
                     RDEVRSGASVGLSGMEERVQLVGGAFSIESTPAVGTEIRAYFPHGVATKGTEDSLV"
     gene            complement(10414..11325)
                     /locus_tag="PK589_03050"
     CDS             complement(10414..11325)
                     /locus_tag="PK589_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNJ18602.1"
                     /translation="MEKPSASAAYAALTISALVWGGSIVGQKLALGAFSAVETSLIRG
                     IGALAILIPLWWWTEGNRTTLTARDLKVLSLLGLFVFGNHLLTLFGLRYIGASTAGVL
                     IGASPAITALLSSLLVRDIPFNQVAGGCAVSFAGVALVSGVGGDAPIGDHPWLGGTLV
                     LLGLVSWALYSIGGRQVMNRLSPLTVNWTTLLLSLLLQIPLLSTDQKMLVTGTSAVPV
                     SGWLAVGYLIVFATALGQQAWLYGVQGVGPSRAGVFVNLIPVSALLLSAVILGEAIGA
                     REIAGIVLILMGVWLVGRQTEPSKRRG"
     gene            complement(11384..12034)
                     /locus_tag="PK589_03055"
     CDS             complement(11384..12034)
                     /locus_tag="PK589_03055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ18603.1"
                     /translation="MADLVSPNIDAYAAACSLPETPVRRALREETERTMEYARMLVGP
                     LEGAFLHVMTRLVRAERVLEIGMFTGYSALCFAEALPDQGRVITCEVDEDSAAVARRF
                     FAQSPHGRRIEIRMGPALETMAELTGPFDLIFIDADKLNYVNYYRRAMELLSPRGVIL
                     VDNVLWGGDVLTDPPPDDRTAAIQDLNRIVAADPRVTAVLATIRDGIWVITHKSSH"
     gene            12119..13639
                     /locus_tag="PK589_03060"
     CDS             12119..13639
                     /locus_tag="PK589_03060"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNJ18604.1"
                     /translation="MKTLKTLEPLTNRLLEIRRIQSAASVLSWDQETYMPAGGGAARA
                     EQIATLEGLAHERLVSPHIESLLNEWIDPSTGQAAEHWDEPSRSLLRETWRDFSRARK
                     LPSDFVIRLSRECSLAQQAWVTARAESQFSKFLPSLRTILDLKCEETQYLGYRDSPYD
                     ALLDTYEPGATVGQLTPLFAQLRARLVPLLKRVQASKVRIDDTCLHHSFDHTKQIEFG
                     RLVLVAMGYDFERGRLDLSAHPFTTSFHPTDVRVTTRVFEKDLPSCLFSCIHEGGHGL
                     YDQGLDPRYYGSPLGESVSLGFHESQSRLWENCVGRSRAFWQCFYPILQHTFPQQLAG
                     VPLDQFYAAINRVTPSLIRVEADELTYNLHIMLRVEIEQALIERRAHPEDLPSLWNDR
                     MEAYLGITPAQDADGVLQDVHWSMGAFGYFPTYTLGNLYSVQFFEQAKLELPQLEEQI
                     AAGQLVPLRHWLEQKIHRWGRMFTPDHLARRVTGNGVTPEPFLRYLEAKYGALYRL"
     gene            14108..14794
                     /locus_tag="PK589_03065"
     CDS             14108..14794
                     /locus_tag="PK589_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ18605.1"
                     /translation="MNAFDTLPAVKTELNILLADDHALLRRGLKEFLQDFLTEDGLTP
                     HITETDSAHSAIDQIRTGAWDLVILDLNLPDMPGLEVLRIVKSLQPSLPILIVSIYAE
                     QHYAARAIRAGALGYLTKDTGPQQLRAAVSRILQGGNYFPPPQSEQVSDTMLDRNTPC
                     AHDLPAVLSDREIEVLQWIAQGRRLTEIAEHLNLSIKTVSTYRARLLTKLGMKTTAEL
                     IRYALDQQLV"
     gene            14929..15315
                     /locus_tag="PK589_03070"
     CDS             14929..15315
                     /locus_tag="PK589_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18606.1"
                     /translation="MANILIINADSNLKRLIKLALPGMEHFVQYSATLHNASAQPPAS
                     KLDLVVARLVADDDQGPEDLVDLRKAFPSSRIVVTAALHEPALEGEKFQRVVRELQIE
                     YCLLEPIETGAMLHTIQSALTLPRRH"
     gene            complement(15449..16012)
                     /locus_tag="PK589_03075"
     CDS             complement(15449..16012)
                     /locus_tag="PK589_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18607.1"
                     /translation="MRKSHEMTVDRALLLYVLSLAEPYGLLSDVKLQQLCFLCELQMF
                     GKGLKGFHFEFVRFAYGAFSRDLDNDLTSLRRKERVENFSPSDQVRDETLPLLLKGID
                     GNETNEKVRDLMQAVIATYGPQDVTAIMQSVESVELSTPQQPDFKIPIRDIVFHTTLL
                     VPSRIEAPAEFLLPANHLAKLNVSMGY"
     gene            16164..16595
                     /locus_tag="PK589_03080"
     CDS             16164..16595
                     /locus_tag="PK589_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNJ18608.1"
                     /translation="MSKNLKEMDVLRQHLAKHQLKLTRQRELILTAFLRQEHVTAETM
                     YHQLAKKDPHLGLATIYRTLNLFCEAGIAQARHFGTQTQYDNISHKGHHDHLICTGCG
                     TIVEFENCEIERLQEEVATKNGFVIQTHRLELYGLCARCRH"
     gene            16687..17718
                     /locus_tag="PK589_03085"
     CDS             16687..17718
                     /locus_tag="PK589_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNJ18609.1"
                     /translation="MASFPRTVFALTLLISALILQEWPAKTADAADKLVVYSGRAERL
                     IKPVLDEFQTKSGIQIELLSSGTTELVNRLQAEGDHTPADVFLTNDAGSLEHARELKL
                     LRPMNMREVERAIPSQFRASDNSWIGLSGRFWVVVYNTTLVKPEQIKSLLDLAQPQWK
                     DKIAIPNSGSEYLQAGVSVIKTTFGDERTKQFLQGLKTNAGSQVYQKSSQIVEAVAKG
                     QVAAGIVNHYYIYRHLAAQPTAPIAAIMTDQQEGGMGAIMNVTGIGVTRASKHVDSAK
                     LLIEFLVAQAGQKLFADLDKEYPLHPDVKADPALVDRHSFRAALVPLARLAELRESTL
                     TLIEQVGLR"
     gene            17734..19338
                     /locus_tag="PK589_03090"
     CDS             17734..19338
                     /locus_tag="PK589_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNJ18610.1"
                     /translation="MLTATRVRAPSSLQWISLFTAAFLVLPTAYIVYVAMTAAPAVWA
                     RLWSTRIPELLGNTLSLALGVALITLVLGVSLAWITVRYDFPGRRIWEWALALPLAMP
                     TYVLAYVYAHLLGMGGPTEQWWQLLFGHEARLPSPQSFPGVTMIMALDTFPFVYLLVR
                     SAIQNLNVSFEEVARACGVSPWATLRRVTLPLIRPAIAAGLALVILYVISDFGAVSLL
                     RYQTLTYAVYQQMTSRYDHTAASILSLLLVVMAIIFLVTERWFRQRSRFYQTSGRYRR
                     STRQHAGPLGTALITSYVVLVFGAAFGIPAAMLIQWSVEAISQGALDTRFLGFIWNSS
                     FLAALAACGAVIIGLPLAYLASRRPSRLNIACLQAAYAGYALPGPVAALAVLVLFTHF
                     APALYGTVVVLIIAYILHFLPVGLQSMEPALQQVTPNVEEVARTLGCTTHQSLWRVTL
                     PLVRNGFVAAWVLMFLQTMKELPATLLLRPVGFDTLAIRVWLEASEEYYQLAAPAALL
                     IVILSLPALMLLVSKDWRGEAQKGVT"
     gene            19335..20483
                     /locus_tag="PK589_03095"
     CDS             19335..20483
                     /locus_tag="PK589_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ18611.1"
                     /translation="MSLVNESITGRQQPASEGIIPNTLDTTVERSAVLELRDVSCAYE
                     PTRPAIEGITFTVHQGEILCLLGPSGCGKTTILRAIAGFERVTRGTIALSGQRVSSPD
                     TMIPTEQRHIGMVFQEYALFPHLRVEKNIAFGLRHLPRTEQQAIVDDLLTLTGLRGLE
                     HRYPHELSGGQQQRVALARALALRPVLLLLDEPFSNLDPDMASRMRQDLHALLRQTKT
                     TAILVTHDHDEAFSMADRVAVLNKGRLEQFDTPEAIYHLPTTPFVADFVGQADFIAGV
                     VTHHLVTTELGDFPNTQHLATGTHVVVMIRPDDIHIVPTKGADARILARQFKGSENVY
                     TIQLPSGQIVHSSESSLSIYQVGTAIALRVVATHTVLFPQPPGSPPVA"
     gene            20583..21014
                     /gene="exbB"
                     /locus_tag="PK589_03100"
     CDS             20583..21014
                     /gene="exbB"
                     /locus_tag="PK589_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HNJ18612.1"
                     /translation="MDVLKNAVEYGIIGLLIALSVWSVAVAVERWLYYRRVNLAQFTD
                     VQTFEMALTKRLVIIGTVAANAPYIGLLGTVLGIMMTFHTMGTSGTMAVNTIMIGLSL
                     ALKATAVGLLVAIPCVVMNNILRRRVAELLTTYKVQHGTRG"
     gene            20998..21372
                     /locus_tag="PK589_03105"
     CDS             20998..21372
                     /locus_tag="PK589_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNJ18613.1"
                     /translation="MEREVNQINVIPLVDVMLVLLVIVLTTATFISTGQIPVNLAKAK
                     EAGDHKDVPVVVTLTANGDLFLNDRPVPADGLKSVMLTHPRESLVVVRADKVTLLERF
                     VSVVDEIRGLGFQSVSLEVVRL"
     gene            22059..22319
                     /locus_tag="PK589_03110"
     CDS             22059..22319
                     /locus_tag="PK589_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNJ18614.1"
                     /translation="MPRIEALKEYPVDARLKHVEGRVVVRIVIQEDGQIVSAAVAKSS
                     GHDTLDQAALETVRKISPIALTQPLEQSPVTLHVPIRYQLGP"
     gene            complement(22400..23500)
                     /locus_tag="PK589_03115"
     CDS             complement(22400..23500)
                     /locus_tag="PK589_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNJ18615.1"
                     /translation="MKLSINSHPLRAFLFMFVNDAVLRRGFALAIRGSLGLLLLLGLG
                     CAGGNLGSSPILVKRTQMHMGTWVSITAVAPDREAAQYAASAGFQEIHRLEELLSTWI
                     KDSELSRVNGAAGKEAVSVSPDTMKVLQASVAMARLTGGGFNILVGPAVEAWSVLDRQ
                     QIPSESELENIRPLTNLNALQLSQAQGTVYLAKPGMRVDVGGIGKGFAADMAVEAMQK
                     AGATAGVVALSGDIRTFGQMPDGTTFPFGIRHPRREGTVLAFVDLQNEAISTAGDYER
                     YFERDGVRYHHVLDPVTLQPARDCQSVTIVAKDGLTADGLDTGIFVMGPERGLALVES
                     LPGVGAVIVDRDGKVWVSSSLKGRVRIKDGVD"
     gene            24076..25554
                     /locus_tag="PK589_03120"
     CDS             24076..25554
                     /locus_tag="PK589_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ18616.1"
                     /translation="MKIRAILGALVLASLPAMPALADNMTPEEIKKLVDEAVEKRLQE
                     HERREGAGDHRETVGAPGNEPGIGSLPEVGKERRTENQAALSFGSTGSGRLVYAKPFV
                     AAPKAIVGGYMDIQYRSHRNGVLESGMGSGTTNGFDQQRFVPFIYADITEHVKFAAEI
                     EIEHGIREDATQGAEIGLEFAHIDYLVKEPFNLRAGILLVPIGKFNLLHDSPLNDLTD
                     RPLVSRLVIPSTMAETGAGFYGTLYPGRTSKLDYEFYVTTGPCGYNGDGSPRIREEDG
                     TRRSRQRKCPSEDGLDINNGKAVSGRVAFSPMLGVEVAGSSYFGNASPLSYNPLSITA
                     IDWTFQRGPFELLGEAAWAYARGNSRAVQGATFATPGSLLSGLNTLNSFAAPPQRMQG
                     FYVQANYHFMPAFLTALSPKRFGEGSTFTAVFRYDRVNLNMDNRGENSGELEQFSFGL
                     NYRPIEDAVFKASYQYMPKSFNPNTGQRIHDSAFVISAATYF"
     gene            25618..26079
                     /gene="bamC"
                     /locus_tag="PK589_03125"
     CDS             25618..26079
                     /gene="bamC"
                     /locus_tag="PK589_03125"
                     /inference="COORDINATES: protein motif:HMM:NF018509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HNJ18617.1"
                     /translation="MQRAHIFIAAAAFLTIGCASLSGSHEQSYSCSQDVVWDAAMDTM
                     KSYSITSQDKAKGVIETGWLEMDGKERSYGIFGRTGFGNRERARMSLALKTSNGAVSV
                     SLLETRQRWHSRGGVTSQATKWWPVEPSEEATAEVTTRLNEKLKQQGCTPL"
     gene            26076..26753
                     /locus_tag="PK589_03130"
     CDS             26076..26753
                     /locus_tag="PK589_03130"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNJ18618.1"
                     /translation="MMPLLLLLTLTGAFALAPLNAQAERVWDNELKRYLTEQEMSLAE
                     VFLTEEEAVKLMFPKSERVRRELLNLPQDKKALIESRIGWKFPEESFEVYIGETGTHI
                     DGYALVQNTIGKHKPMTYMVGVDAHGLVSNVELLTFREARGSEVRTKRFNVQYEGKSV
                     LDPVRINKDIINISGATMSVRSMTAGIKRVLVLVDEFYLKPQGRGSDTVTAQKTEKGF
                     FKSIFGN"
     gene            26766..27284
                     /locus_tag="PK589_03135"
     CDS             26766..27284
                     /locus_tag="PK589_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18619.1"
                     /translation="MRNFNTRFRLRDSDRHIRLVYTFFLLLMLAGFTFSFFWAHSMTG
                     LSPQGIADHYRGSNATFGEPMSFRELAEITHFHLFTMPVVFMILVHVLYLTNASNRLK
                     IVTTWLSFGGVILDLLSPWLISYVSPVFVLTMLTGDTFMTAGFFIMFVIPLHEMWILK
                     QPLMAGKRGEHA"
     gene            27390..28145
                     /locus_tag="PK589_03140"
     CDS             27390..28145
                     /locus_tag="PK589_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNJ18620.1"
                     /translation="MHRSTRPIPTTSQLIQDLATQLSALNTLQLSPESLEAVRVIQLE
                     RLARRIETVIPGHFGHGERTAHYALLLADRLRLTKHQRLDLHYAALLHDVGLLMMPGH
                     LLGAPAPHTLNDYALIQSHPREGAALLTPYTFLQVAARLIAHHHERWDGAGYPYGLRG
                     EYIPASARILAIADVFDSITSRTNSWDSALRTLHASAGSQFDPAFCVTFIELLRNHDH
                     YRAYQSSDLSSDAMHSQSSPEHVPEAYMTGSGI"
     gene            28203..28763
                     /locus_tag="PK589_03145"
     CDS             28203..28763
                     /locus_tag="PK589_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNJ18621.1"
                     /translation="MMSGKSSTSNISFDRLHSFSSELRVQSPQWADRLEQVRTEQDLG
                     DLICDLFNLSHAINPETQPPPIPHTQPSRIAAFVNENLHRGVTLKVLANFLGYSEKYC
                     SDLFCRIMGEPFSKYLRRQRVERARALLITTRQTLTEIASALGFSDQFAFSHFFKRAT
                     GQSPMAIRLTIARQQRQTIQGLQQTS"
     gene            complement(28871..29350)
                     /gene="bfr"
                     /locus_tag="PK589_03150"
     CDS             complement(28871..29350)
                     /gene="bfr"
                     /locus_tag="PK589_03150"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNJ18622.1"
                     /translation="MKAKEGVLDILNKVLVSELTAVHQYLLHAALCKHWGYARLHEHF
                     SHLAQEEVSHSSGLIDHILYLGGSPEMGRLDAVAAGKKVSELFSADLAFEREDADALR
                     QGIAHCAKVGDFTTRHRLEDMIIDTEEHIDWFETQLRTIEQVGEDRYLAEQLTADKS"
     gene            complement(29385..29861)
                     /gene="bfr"
                     /locus_tag="PK589_03155"
     CDS             complement(29385..29861)
                     /gene="bfr"
                     /locus_tag="PK589_03155"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNJ18623.1"
                     /translation="MKAKQGVIELLNKVLTADLTAINQYFVHAKMCANWGYERLHHKV
                     RERSIDEMKDADELISHILYLEGVPNVQRMNTVHIGETVPEQLKLDLKAEQEMLALLS
                     EGVVHCTKVADFTTRHMFEDMAKDVDEHIDWIETQMETIKQIGLENYLAEQIKKDS"
     gene            complement(30169..32508)
                     /gene="topA"
                     /locus_tag="PK589_03160"
     CDS             complement(30169..32508)
                     /gene="topA"
                     /locus_tag="PK589_03160"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNJ18624.1"
                     /translation="MAKSLIIVESPTKARTITKYLGRGYSVMASVGHVKDLPTSKLGV
                     DLEHDFEPQYVTIKGKSKVLADIKKKAQQVDTVFLAPDPDREGEAIAWHIAQELHGKG
                     KKKDGKVYRVLFNEITESAIKRALKSPGEIDMKLVQAQQARRVLDRIVGYQGSQLLWK
                     KVRRGLSMGRVQSVAVRLICDREQEREKFRAEEYWSIVALLRGDNPPAFEAKLHSING
                     QDADIGTSAQAEQILNAVQGKAFVVQSIERKEKKRNPVAPFITSRLQQEAARKLHFTP
                     KKTMTLAQQLYEGVEIGAEGPMGLITYMRTDSPRISQEATEDARLVIRERFGEEYLPA
                     TPNVYKTQKAAQEAHEAIRPTMAARDPESIRQFLEQDQYNLYKLIWNRFIASQMVPAI
                     LDVTRVDSSLIGTPERYVFRTTGTVVKFPGHTAVYLEGTDKELPSQKPKSEQESEDES
                     DRQLPVLREGESLHLVTQEAQTLPGLISKQHFTQPPPRYNEALLIRELEEKGIGRPST
                     YAAIISTIQDRKYVEKVEGRFLPTETGRTVNDFLLKGFPDLLDTEFTSHMEEQLDEVE
                     EGTKPWVSAVREFYAPFVREMELAQSIPGPKDIVEPPTNLPCEKCGRMLEIKWGRNGK
                     FLACPGYKEDPPCKNTQNFEKLPDGTIKIVPKLEETTDEKCEKCSSPMVVKSGRFGKF
                     LACSAYPECKTTKAIALGVKCPQPGCGGDLVQKRTKKGRNFYSCSRYPECEFALWDRP
                     INKPCPKCQAPFLIEKVSKQAGRSVQCRNEEECGYREAG"
     gene            complement(32604..33725)
                     /gene="dprA"
                     /locus_tag="PK589_03165"
     CDS             complement(32604..33725)
                     /gene="dprA"
                     /locus_tag="PK589_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNJ18625.1"
                     /translation="MVNSLSLRPWLMLRAIPGVGDAILLKLVQAMGSPDAVLSATQAS
                     LEDVGCRVPLIDAIRRGPDAQAVKQLDRELAAVQHRQITVLTYLDPQYPAPLKTIPDP
                     PPLLYVQGTLLESDRQAVAIVGTRKVSSAGRVLAEELACELAEMGFTIVSGLARGVDA
                     AAHRGALAGKGRTLAVMGCGLDRTYPADHAALRQEIERHGAVLSELPLGSAPHSYHFP
                     RRNRIISGLSLGVVVTEAAVESGSLITARLAGEQGREVFAVPGFVKAENSRGPNSLLK
                     DGARLVESAQDILDELLPQLDAAFRERLGDRARGARPAMQLGPEEAMVYDAVSVLPQS
                     VDDVIRRSGLAAAQVTAVLLSLELKNCVRQLPGNEYVRL"
     gene            complement(33835..35346)
                     /locus_tag="PK589_03170"
     CDS             complement(33835..35346)
                     /locus_tag="PK589_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNJ18626.1"
                     /translation="MGVEIAISIAREETRVAVLDNGVVTDLYGDRAKDQDYVGNIYKG
                     RVAKVLPGMQAAFIDIGLEKAAFMHVSDLSIDVEPGDTLVEGDDEDNKDSEVPKPKRQ
                     SSKPIEELLSEGQELMVQISKGPIGTKGSRVTTYVSLPGRYLVFMPTVEHIGVSRRIP
                     RDEERARLKEIMKRARRPGFGYIVRTVSEGVKEEELRSDVEFLHVLWQDVLTKRDQQP
                     APSLLHSDLSLSFRVVRDLFGRRVDRLLIDSREEYNAIKGFVQRFSPEQTSRIHFYDK
                     EESLFDYLGVEQEIARAMSRKVWLKSGGYLVIDHTEAMTVIDVNTGRFVGKRDQEETI
                     LRNNLEAAREIAYQVKLRGIGGIIIVDFIDMEREKNRDKVYHALVDAMSSDKARTRIS
                     RVSDLGLIEISRERVREDLLRSLSEPCHYCDGRGYTKSPMSVAYEIFREVRRLGHEIE
                     QQRVVVGAHPAVALLLQEQEQPGVEELERRYSAKILVTPDDRLHLEQFDLVVM"
     gene            complement(35489..36607)
                     /gene="rodA"
                     /locus_tag="PK589_03175"
     CDS             complement(35489..36607)
                     /gene="rodA"
                     /locus_tag="PK589_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNJ18627.1"
                     /translation="MIDRVIDSRGLDSFDLRFMGLIAVILSVGVLSIYSVTHSQDTAF
                     PFYLKQLVWILLGTVAFLVMYLSDYHKIARLAYPTYAVILIMLAVVLFMGKSSRGAQR
                     WIPIGPFAFQPSEFAKLVLVLVLANYYSRASRVGWLHRVVLPGLIVLPGLLLILKQPD
                     LGSGLSFLAVYAAMLLMVGVRSKALGIILLLSVMLFPFVWEVVWASLHDYQRERVMAF
                     VDPDYDPGGKGYHALQSRIAIGSGELSGKGLYGGTQSQLKFLPEGHTDFVFAVYAEEW
                     GFVGVLVLLALFIALIWVSLEIAARAKDTLGALLAAGIVAMLCFCVVVNIGMTAGMFP
                     IVGIPLPLVSYGGSATIMTMASLGLLLNVKRRRLTLFY"
     gene            complement(36607..38490)
                     /gene="mrdA"
                     /locus_tag="PK589_03180"
     CDS             complement(36607..38490)
                     /gene="mrdA"
                     /locus_tag="PK589_03180"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNJ18628.1"
                     /translation="MATVGFNDSDLLDLQRRLVILRVGLLLVVALLALRLWHLQIREG
                     PYYRDLSENNRTRSVVLEPARGLIFDRNGVLLANNVPSFALYVTLEDVKDRQALVGQL
                     ASLLALDPEVIQKKLSTGKGGKLQPRKVKDRLTLREATLIESHRLDLPGVMIQVESQR
                     NYPAGAVAAHLLGYVGEVSADQLEKPDFADLHQGSIVGQYGVEKWFDRQVRGRAGQKS
                     VEVDALGHEKRAIVSDKPLAGDDLYLTIDAKLQKVAEDLLGDESGAIVALDPTNGDIL
                     AMASRPGFDPNVLSKELTNKQWVEIVQNERRPLNNRATQGQYPPGSTFKVVMAAAALE
                     SNTVTPSTMVRCNGGYQFGNRLFHDWKQGGHGSVDMNEALIHSCDVYFYTVGQRMGID
                     TIAEYAKQFGLGQETGVELPSERVGIVPSTAWKQKARNQPWYPGETISAAIGQGYVTV
                     TPLQMASVIGTVANDGVSIKPRLVQAVMNRTTGERAEFPPTVRGKVAVKPATLALIKS
                     ALADVVTKGTATRAKSPLVTIGGKTGTAQTAALRTGPEKDIPKKFRDHAWFVAFAPVE
                     APRIAVAVLAEHMGHGGSAAAPLAKDVIEAYVKAYPQVLQGAPASGRAKSPAVPVDTR
                     PKT"
     gene            complement(38532..39032)
                     /locus_tag="PK589_03185"
     CDS             complement(38532..39032)
                     /locus_tag="PK589_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18629.1"
                     /translation="MKFLLYLLLALVVVPLQTTLLHYASVFGVRPDLGLIAACLVGFL
                     GGELDGLILGLILGCFQDMLSAGDLWINVVTKGGSGFLAGLAGRHMAHITPAVMTAGL
                     AIISCLSSAVFLYSMNPAGLDEVWLGVRSTVLVQAGLDAALGAGLYLLFRQRWSDDRM
                     VTEGAL"
     gene            complement(39029..39910)
                     /gene="mreC"
                     /locus_tag="PK589_03190"
     CDS             complement(39029..39910)
                     /gene="mreC"
                     /locus_tag="PK589_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNJ18630.1"
                     /translation="MAISRSTYGTRRLAIVLFACLLVALFLLPSQSQRLLQYVGGPLG
                     QVLSLPLAAFSSVDHGIADMWNGYVALQTVREENRQLHRDIELLKGQNNQLRESMTAV
                     QRYEALLNFKQQSPSQTLAAQVIGRDATNWYRGMILNKGENDGVRTEMGVITPAGVVG
                     RVVKTNATSSVVLLVTDPNNAIAGVVQRTRDEGIVEGTSHGRARLKYIPLLSNVQAGD
                     RVVTSGLTGAFPRGLAIGSLIQVEKSEGDLFQSAEIEPEVDLSKLDEVLIITAPYEDA
                     DAAQKLLQAIHGDRKKP"
     gene            complement(39929..40978)
                     /locus_tag="PK589_03195"
     CDS             complement(39929..40978)
                     /locus_tag="PK589_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNJ18631.1"
                     /translation="MAFMSDMFGWFSNDLAIDLGTATTLVYVQGKGIVLNEPSVVAVE
                     KKTERVMAVGADAKRMLGRTPGNIIAVRPMKEGVIADFEKAEAMLSHFIQKAHNRTAF
                     VRPRIIIGVPSRITQVEQRAVRDSAELAGAREVYLIEEPVAAAIGAGLPITEPSGNMV
                     VDIGGGTTDIAVISLGGIVYSESVKVAGDRMDDAIMNYIKKKYNLLIGEHMAERIKFE
                     IGSAYPFEERKTMMIKGRDLISGIPRTLVVDDAEVREALQEPIGTIVNAIKVALENTP
                     PELAGDIIDRGIVLTGGGSLLKGMDTRFREETNLPIITVDDPLTSVVLGVGKILDELD
                     LLRKVSVMSQCSTSR"
     gene            complement(41027..41644)
                     /locus_tag="PK589_03200"
     CDS             complement(41027..41644)
                     /locus_tag="PK589_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18632.1"
                     /translation="MRLFSAGFRGFYSGDDSKGKRKKCAESGCEKIATLFVQVKEICY
                     SVEELGRFPVAEEAIGRSPVELGVYPKAQVLNRFIAKMIDLLIVAAVSKLVPPIGVLA
                     GLAYILLGDGFGGGRSVGKRLIGLQTIVPRTRDPAGFRESIIRNLPCGLAQLAFEIPY
                     VGWIGWGAVLSLEGLLVIGNEQGRRLGDEIAKTQVLESGQLDVSD"
     gene            41648..42346
                     /locus_tag="PK589_03205"
     CDS             41648..42346
                     /locus_tag="PK589_03205"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNJ18633.1"
                     /translation="MIKLLREAAHDYPWFLKSIMGTLALAFVITMGWWGFGDQSGNVV
                     ASVGDQVVPLDEYRRAYENTYRFYKDKGQNDIKDEFLKQFVLEQLIDNRMWLHVAKEM
                     GLSVSDEDLRKAIMQRTEFQKNGNFDPESYRRLLAANRLTPASFEAMEAKDILTNKAR
                     LVIMDAVALTPSEYAEAQTLVSREGESDPTKAAIAKERIFQNLLFQKQQRALMAYSES
                     MKSKVPVKIHKELM"
     gene            complement(42339..43379)
                     /gene="queA"
                     /locus_tag="PK589_03210"
     CDS             complement(42339..43379)
                     /gene="queA"
                     /locus_tag="PK589_03210"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNJ18634.1"
                     /translation="MLLSEFDFPFDPALVADRPVLPRHDARLLVMDRSSHARMHRRVA
                     DLPSLLQPGDLIVVNNTKVMPARVPAQTRSTGKILDLLFVQDLGQGLWEVLIKGRFKP
                     GAFIDFPGGGCGEIVERSQARTTVKVTGVTSVYDLMHNTGTMPLPPYIKREPSQDDQR
                     WYQTMFAQQEGAIAAPTAGLHFTPELVETLTGKGMELAEVTLHVGPGTFRSVKSDRIE
                     EHRMLAEQVEVTARTVEQIRLAQKRGNRVVAVGTTVVRALETAGRGGRGIQPFQGPAE
                     LFITPGFEFQVIDALVTNFHLPRTTLLMLVSAFVGLEPLRAAYEEAVSERYRFYSYGD
                     AMLIAGGLSAAT"
     gene            complement(43379..44530)
                     /locus_tag="PK589_03215"
     CDS             complement(43379..44530)
                     /locus_tag="PK589_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNJ18635.1"
                     /translation="MALAGIVFAQSASAESIRVLLVQEAPFVDVRSDGPLALVMETGD
                     TKTLHGPIHLTARADGLHVDGRRILGGQVLIRSLRHNLTLVVGKEGGSAGTPLPISGT
                     VRVSRKGHAVAVVNQVDLEEYVKGVVPSEVSSAWHPEMLKVQAVAARTYALYNKMLSA
                     ARDYDVMATIQDQVYRGRTGVDHRVEEAVESTRGIVVTHQQAPIYAAFSSTAAGPTED
                     AVNVWANKDLPYLKGVECPFDLESPYYQWKASVKIEQLEKNLRHQGFAVGTIATITPI
                     AFSRAGRVARLRILHSGGETLLRGEDLRKAAGYTVIPSTQFEVESIGAEVVFAGYGAG
                     HAVGLCQWGAKELAELGYSYNSILQYYYPGTELRNSALSQLMMPVIPTP"
     gene            complement(44569..44823)
                     /locus_tag="PK589_03220"
     CDS             complement(44569..44823)
                     /locus_tag="PK589_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNJ18636.1"
                     /translation="MGAWSGIGRLFIVAGLALAGVGILLTLLERWSGAGAAFGWIGRL
                     PGDLSITRERFSLYVPITTSLVLSILLSVVLYILSWIFRR"
     gene            complement(44827..45846)
                     /locus_tag="PK589_03225"
     CDS             complement(44827..45846)
                     /locus_tag="PK589_03225"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNJ18637.1"
                     /translation="MLKKKQAYTVAVLGATGAVGKESLEILEERNFPIETLRLFSSKR
                     SAGEVLTCQGKEYKVEELTEASSFAGVDIAFISATDAISRDYGARLGAAGIVVIDDSA
                     VFRMDPDVPLVVPEVNAAALQSMKRGIVAIPNCTTTPLVMALKPLRDVAGIKRVVVTT
                     FQSVSGTGSAAMDELVDQTKALISFQDVKVQVYPYQIAFNLLPQVGSFGDGGDCSEEV
                     KIVQETRKILDMPTLRVTATTVRVPVLRCHSEAINVELERPMKANDARAAIAEMPGVI
                     VYDDPVKKLYPMPFDVSGKDEVYVGRVRVDESITNGLNLWVVSDNLRKGAALNAVQIA
                     ECLIQ"
     gene            complement(45857..47044)
                     /locus_tag="PK589_03230"
     CDS             complement(45857..47044)
                     /locus_tag="PK589_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18638.1"
                     /translation="MIATLAGNGEPGYAGDGGPAAVASLNEPKGLCIDREGNVYIADS
                     ENHVVRRVDRKTGLITTVAGVGPGGTGGAMMAPEPVAMPPQEEEDPFADTSGDSTKAY
                     TQVTDLSGTVRYVTGGRLTIEQESGDGGPARRARLNFPSAVAVDRAGNLYIADTMNHR
                     VRKVDAKTGIITHVAGTGQARYSGDGGPAVQAAINEPTGLAVTDDALYIADQSNNRVR
                     RVDFATGVITTAAGDGSAAYSGDQVPAVQASLAGPSGVAVGNDGLLYVADTFNSRIRS
                     VDPATGQITTAAGDGGTYRYQGPEEPASPSLSRPAGLAVDYDGNVYITDSDSHLIRVW
                     NGRTKTITRVSGTGVAQFGGDGGNALAGSLSYPFGVAVDAAGTVYVADTFNHRIRVLT
                     ATA"
     gene            complement(47075..48151)
                     /gene="leuB"
                     /locus_tag="PK589_03235"
     CDS             complement(47075..48151)
                     /gene="leuB"
                     /locus_tag="PK589_03235"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNJ18639.1"
                     /translation="MKAKIAVLAGDGVGREIVPEAVKVLTTVAERFGHTFEFQAADVG
                     GHAIDKVGVPLPAETLTLAKHSDAVLLGAVGGPKWEGLDYSIRPERALLGLREQLGLY
                     ANLRPAKLYPMLADASTLRREVIEGIDMLVIRELTGGIYFGKPKGVEKMPGGGERGFN
                     TEVYTTEEIRRIAVVAFEAARKRRKKVMSVDKANVLESSELWRRVVTEVQKDYADVTL
                     SHIYVDNCAMQLVRNPRQFDVLLCNNIFGDILSDEAAMLTGSIGMLPSASVGAKVSLF
                     EPIHGSAPDIAGKNIANPIATIASGAMMLSYAFQLDKEAAAIEQAIVKTLEQGFRTKD
                     IHAEGMRLVGTTEMGDAIVKNLPA"
     gene            complement(48170..48532)
                     /locus_tag="PK589_03240"
     CDS             complement(48170..48532)
                     /locus_tag="PK589_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNJ18640.1"
                     /translation="MFSTHLQQCPEFLAGDHTRLRELLHPGKAELELGYSLAHGLLDP
                     GKQSLWHRLKSSEVYYFIAGRGVMKVEEASMPVQAGSVIYVPPGAKQSLINTGTESIE
                     FLCLVDPAWKAEDEAVVE"
     gene            complement(48635..50182)
                     /locus_tag="PK589_03245"
     CDS             complement(48635..50182)
                     /locus_tag="PK589_03245"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNJ18641.1"
                     /translation="MTRMIRIFDTTLRDGEQSPGASMNVEEKLMIAKQLARLGVDIIE
                     AGFAYSSPGDFEAVRRIALEVEGPVVCSLARARPEDITRASEALKGAPRVRIHTFLST
                     SDIHLKHQFRMTREEAKKRAVEMVQLARTYVDDVEFSPMDASRSDPAYLCEVIEAVIA
                     AGAGTINIPDTVGYAVPQEFGGLIKRIKDTVPNSSQAIISVHCHNDLGMAVANSLAAV
                     VAGAGQVECTINGIGERAGNTSLEEIVMGLRTRRDWYGADTKVVTEEISKTSRLVSKI
                     TGMVIQPNKAIVGANAFAHTSGIHQDGLLKDKTTYEIMRPESVGLEQVKLVMGKLSGR
                     HAFRQRLEDLGYKLTEDEVNHAFERFKRLADQKKEIYEEDLEVIISEEVAKMSERVVL
                     KSFQVESGTNKTPKATVELEIDGKVVSHSGTGDGPVDAVYRTIAAMTQTKSKLLMFGV
                     NAITGGTDAQGEVSVRLEEDGRTVSGHGADTDIITAAARAYLNALNKLAYFAAKQAEG
                     IHKVSLI"
     misc_binding    complement(50353..50597)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(50594..51397)
                     /gene="pssA"
                     /locus_tag="PK589_03250"
     CDS             complement(50594..51397)
                     /gene="pssA"
                     /locus_tag="PK589_03250"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNJ18642.1"
                     /translation="MKSPTMRPPFAKGHRKRQAMYLIPNLCTTGNLFCGVFAILSVFN
                     GQHLVAAIAILVGMIFDMLDGKLARLTNSTGQFGIEYDSLSDVVSFGVAPGVLIYSYA
                     LSGQGMFGVAVMFAYVAMGAVRLARFNATVSTSDSKYFTGLAIPAAAGVIASLVIFDL
                     HITQLGSEVKPLLILVITFGLAFLMVSTIKYRSFKDMKFRRGDHFTYLVWGILALMLI
                     VAWPQAMLFVTFTGYAVSGPIARLWTMIAKSAGKPVAKTDGPVVETRES"
     gene            complement(51394..52044)
                     /locus_tag="PK589_03255"
     CDS             complement(51394..52044)
                     /locus_tag="PK589_03255"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNJ18643.1"
                     /translation="MADRAVGIPIVKEGLPFVGGLAGATLLFGLAGWTIPTVVAAGFL
                     LFTAWFFRNPSRTIPQQPNVVVSSGDGKVIAIEEEFEPRYLKEKSIRITVFLNVFDVH
                     VNRMPCAGTVEGVSYQPGQFLVASKPEATLHNEQNAVMLKTESGAKVLCVQVAGLIAR
                     RIVCWVGQGDRVQRGERFGLIRFGSRMDTFLPLGSKICVALGDRVKGGETIVGELR"
     gene            complement(52094..53107)
                     /gene="ilvC"
                     /locus_tag="PK589_03260"
     CDS             complement(52094..53107)
                     /gene="ilvC"
                     /locus_tag="PK589_03260"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNJ18644.1"
                     /translation="MKIYYDKDADLQLIRGKKVAVIGYGSQGHAHSLNLKESGATVVV
                     GLREGSSWKKAEASGLKVMPVADAVKASDVIMILAPDEAQAAIYRQDIAPNLKPGSYL
                     AFGHGFNIHFGQIVPPADVNVFMVAPKGPGHLVRSEYTKGSGVPCLLAVHQDPSGNTR
                     QVGLAYASAVGGGRAGIIETNFREETETDLFGEQAVLCGGLTSLIQAGFETLVEAGYA
                     PEMAYFECLHEVKLIVDLIYQGGIANMRYSISTTAKYGDITRGPRVVTEQTKQEMKKI
                     LGEIQSGQFAKEWVLENQANRPVYNALLKKGEGHPIEEVGGRLRSMMPWLKKDQLVDK
                     NKN"
     gene            complement(53174..53692)
                     /gene="ilvN"
                     /locus_tag="PK589_03265"
     CDS             complement(53174..53692)
                     /gene="ilvN"
                     /locus_tag="PK589_03265"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNJ18645.1"
                     /translation="MEHIISVTVENKFGVLSRVAGLFSGRGFNIESLSVAPTLDPSMS
                     QMTIVTSGDERIVEQIVKQLNKLIDVIKVVDLNESEFVSRETALIKVHTKAEDRAEAL
                     RIADIFRANVIDSTPSTYTIEVTGDPKKLEAIINLLQPLGIKELIRTGRVAIAREAIR
                     PALSQPKKLARE"
     gene            complement(53735..55513)
                     /gene="ilvB"
                     /locus_tag="PK589_03270"
     CDS             complement(53735..55513)
                     /gene="ilvB"
                     /locus_tag="PK589_03270"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNJ18646.1"
                     /translation="MKLTGSEILIECLKAEGVKTIFALPGGVVLKIFDMLHQQKDIEV
                     VLTRHEQGAGHMAEGYAKATGKAGVCLVTSGPGMTNVITALADAYMDSVPLVCISGQV
                     STSLIGNDAFQEADNIGLSRPCTKYNFLVKDVNDLATTIKEAFYIATTGRPGPVLVDI
                     PKDVSMAKADFTYPSSVSIRGYNPTYEGNKWQIKQAAEAIMKAKKPILYVGGGVVFSQ
                     ASKELLELAEMTQIPVDMTLMGLGAFPGEHPQSMGMLGMHGTYCANMAVHYSDLVIAV
                     GARFDDRVTGKVSEFCPFAKVIHIDIDPTSIRKNIHVDIPIVGDCKAVLRELNQILRA
                     TVNGNQKDLRKPWWDQIREWQQAHPLAYQQEPDGAIKPQHVVKRLYELTKDRDPIVAT
                     DVGQHQMWTAQYFKLARPNRWLTSGGLGTMGFGFPAAMGAQAAFRDRLVLCVAGDGSI
                     QMNMQEMATAVVSKLPVKIIILNNRFHGMVRQWQDLFYEGRYASSYLDTTPDFVKLAE
                     AYGAVGLRAGKVGDLDAVLKEAISTDRPVVVDVPTYPYENCYPMIPAGGCNHEMILAD
                     PPELKQRMAGVPSTGSDENKDTILTA"
     gene            complement(56006..56429)
                     /gene="rnpB"
                     /locus_tag="PK589_03275"
     ncRNA           complement(56006..56429)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK589_03275"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(56491..57381)
                     /locus_tag="PK589_03280"
     CDS             complement(56491..57381)
                     /locus_tag="PK589_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNJ18647.1"
                     /translation="MKMKSNREGWSKSARPSNLLWLCFGIVTMLVTTGCQTNPYTGRW
                     QLMMMPMSQEVQMGAQAYADVKSDPKMRPSTDPREIEPVKRVAARVIEAAKRSKYSDV
                     ANEFEWEVTVIKDDKTMNAFALPGGKIAVYTGIFPVAKTEAGLAAVMGHEVVHALARH
                     GGERMSQNTLAQTTLQAIGIALGVSGANPVLSQAGMAALGVGAQVGVLLPFSRKHESE
                     ADYVGVLLAADAGYDPREAIQLWQRMGEMSGGKSPSEFMSTHPSHETRIQQLEEWMAE
                     AMPIYQSKPPAPNHELPALH"
     gene            57467..58126
                     /gene="rnhC"
                     /locus_tag="PK589_03285"
     CDS             57467..58126
                     /gene="rnhC"
                     /locus_tag="PK589_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HNJ18648.1"
                     /translation="MPITTTLFSLNRIGIDESGKGDYFGPLVIAAVFVTPASEQDLAL
                     MQVRDSKKISDGRILEMAPDIRTVCPHSIVAIGPQRYNELYAKINNLNRLLAWGHARA
                     LENLLQQVDCDLAIADQFGDERLILNALQEKGKQIRLVQRTKAETDLAVAAASILARA
                     EFLLRLQRLSQELHTTLPKGASPAVELAGRMVVKKYGRDRLSTVAKLHFKTTQQVLND
                     T"
     gene            complement(58123..58899)
                     /locus_tag="PK589_03290"
     CDS             complement(58123..58899)
                     /locus_tag="PK589_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNJ18649.1"
                     /translation="MNPQLSPLIELQKLDLRIADLKEQRRRIPERLEVNEAPLREARR
                     TVQDASASIETLNKERRSHEKDLEAHEDRIGKMKDRAAQLKTNQEYQAHLFEVELANK
                     KRGEIEEKILLAMEQIEQTQRMIASAQVQLKDSEALFSKEKAVLDEKDRVLAAELAEL
                     EAQQKELSGRVEKGLLARYNKLKATRKDQALALIKDGICIGCRLQVPPQLISQVKRGD
                     DVYTCPYCYRMLYWEGEPVPEGKRPLVQDQAKDFELGESV"
     gene            complement(58977..59053)
                     /locus_tag="PK589_03295"
     tRNA            complement(58977..59053)
                     /locus_tag="PK589_03295"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59017..59019),aa:Ile,seq:cat)
     gene            complement(59166..60971)
                     /gene="rpoD"
                     /locus_tag="PK589_03300"
     CDS             complement(59166..60971)
                     /gene="rpoD"
                     /locus_tag="PK589_03300"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNJ18650.1"
                     /translation="MPKQELLGEVKKLINLGKEKGFLTYDDLNSTLPADVVSSDQFST
                     IMTMFGEMDIEIVESAETERAPKAAEGEEAIEESEESDSSEENEKEIDLTPGALSRTD
                     DPVRLYLKEMGSVALLSREGEIEIAKRIEEGKKDIATVVYGLPMTLEFVLNLRDQLKN
                     GKIDVREIVPVVETEEDFEEEAVEKDYEELRVKTLDSLNAVRKVSASLKDLYDKARHV
                     GADPEKQKKLRKQIDTVRGQVVDKMESVNLHGVLKDRMTQRVRDLNLLFRQSEREVTS
                     CQRRLGVGGEAGAELLRKLCRTHKDVLAVKRRTGLSEEVLQDIKKHYQAAKGRIRQLE
                     EEEALISAEEIKDAVKHLDVAEEKVKRGKAELVEANLRLVVSIAKKYTNRGLQFLDLI
                     QEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKL
                     IRTSRHLVQKLGREPTPEEIAERMDLPLDKVRKILKIAREPISLETPIGEEEDSHLGD
                     FIEDKKAVSPLEAAIRYDLQRQINGALETLTPREEKVLRKRFGIGEATDHTLEEVGQD
                     FEVTRERIRQIEAKALRKLRHPSRSKKLRSFVESL"
     gene            complement(60975..62819)
                     /gene="dnaG"
                     /locus_tag="PK589_03305"
     CDS             complement(60975..62819)
                     /gene="dnaG"
                     /locus_tag="PK589_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNJ18651.1"
                     /translation="MGQGLISDDVINQIRDRVDIAEIVGQHVALTRAGQNLKGLCPFH
                     QEKSPSFTVSSSRQIFHCFGCGAGGNVYTFLMRLTGAAFPEIVRDLGRKVGVDVPEST
                     GGVSNEARTQLGRLERLNAAAGAWYHRMLTQGPTGAQARAYLDSRGIQQHTTAQFNIG
                     YAPPEWDGLTRAMLKEGFAPADLVAAGLSIPRDQSGSQKTAVSGHYDRFRSRIMFPIT
                     DLRKRVVAFGGRILGDGMPKYLNSPETPLFKKGQTLFALDAAREAAGQQHTLIIVEGY
                     FDAIALHQAGIRHVAATLGTALTAEHITVIRRFASNIVLLFDPDQAGVRAALRSLDLF
                     VNSGLSVKVVSLPEGDDPDTYVRQHGAEGFAALHRAAPSLLDFAIEHSLRTAESGSVE
                     DRIRAVDAVLRILQKSAHPIEREERIRLVAERLGLSQQRLIDRYPTLVSEEQRRPDRT
                     AKAPSISVPSRVKPNPEERDLAHLLVQGSLSAADLRKLSPDAFSAPAYKRLIECALQH
                     LADDGRVSVRTLLDALITDEVCGSLVSELSMQEQHYDDVPAHIAGCLETLERRGRERT
                     MGLLIQELKVAERERREDEVHRLNGLINDMRIRKAGVMPVALTSAVKE"
     gene            complement(62951..63295)
                     /locus_tag="PK589_03310"
     CDS             complement(62951..63295)
                     /locus_tag="PK589_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNJ18652.1"
                     /translation="MDNCIFCRIVNRAIPAKIVHEDDRVLAFEDVNAQAPVHILVIPK
                     RHLAAVQNCQDGDQALLGHLLMTCSKVAAMKNLAESGYRIVTNTGADSGQTVFHLHLH
                     VLGGRHMAWPPG"
     gene            complement(63288..63971)
                     /gene="hisIE"
                     /locus_tag="PK589_03315"
     CDS             complement(63288..63971)
                     /gene="hisIE"
                     /locus_tag="PK589_03315"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNJ18653.1"
                     /translation="MSSDSRSMTFDGQGLIPAVIQDWFDGTVLMVGYMNQEALTKTLA
                     TKSVHFWSRSRQKLWEKGETSGHFLRVKDLFVDCDRDTILVKAEPVGPTCHTGERACF
                     FSRMSADGRAEPGKTQDAGGGILERLYQTILARKAAPQPDSYVSKLLQGNVDRILKKV
                     AEEAGEVIIAAKNQKRDEIIYETADLFFHTLLVLGYHDVTLSEVYRELGARFGKSGIR
                     QAPEGESRG"
     gene            complement(63984..64754)
                     /gene="hisF"
                     /locus_tag="PK589_03320"
     CDS             complement(63984..64754)
                     /gene="hisF"
                     /locus_tag="PK589_03320"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNJ18654.1"
                     /translation="MLTKRIIPCLDVKDGRVVKGVSFVNLRDAGDPVEVATIYDREGA
                     DELCFLDITASHENRKTIVDVVERTAARVFMPLTVGGGVRTLDDIRTLLNAGADKVSI
                     NTTAVQKPEFVREAAQRFGTQCIVVAIDAKRAEAAGRWQVYTHGGRNATGLDAVEWAQ
                     RMAEYGAGEILLTSMDQDGRQNGYDLALTAAVSESLSIPVIASGGVGTLEHLYDGLVK
                     GKAEAVLAASIFHYRTHTIQQAKAFLRDRGVPVRIGAA"
     gene            complement(64825..65550)
                     /gene="hisA"
                     /locus_tag="PK589_03325"
     CDS             complement(64825..65550)
                     /gene="hisA"
                     /locus_tag="PK589_03325"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNJ18655.1"
                     /translation="MRVIPAIDLKDGRCVRLRQGDMAAETVYSEDVPSVAKRWQEQGA
                     DLIHIVDLNGAVDGEPKNLPQIESAMKAVNVKVQVGGGIRTIDTVRKYLQAGVARVVL
                     GTAALTDRAFLAQACREFPRRILLGLDARDGKVAVKGWTAVSETKAIDLLRELADHEL
                     GAVIYTDIARDGMLNGPNLAALGEVVNCAAFPVIASGGITRVEDLQAVRALGPRVEGA
                     IIGKALYDGKLDYRAALAALGNR"
     gene            complement(65573..66310)
                     /locus_tag="PK589_03330"
     CDS             complement(65573..66310)
                     /locus_tag="PK589_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18656.1"
                     /translation="MSTCIPRTLFILLSVVLTTGCSGTKVTSKASPQAGKYQVRNIVL
                     IPFETMATPQAIETSGPSFPVPSGVRRSDMAVGVPPTTDQAVRRTNLVPPGAGEKITD
                     LMWAKLKGKPGLQVISPQEAMGAARDLSRGSGSVETIPAHKIAQRLKADAALSGKVLV
                     YQERVGSRLGADPPAAVGFEVKLVAPDGMVLWEGNYYEKQRPMTEDVLGFVQRYGTFV
                     TAEELAAYGATELAEAFPFGAAAGEGK"
     gene            complement(66307..66915)
                     /gene="hisH"
                     /locus_tag="PK589_03335"
     CDS             complement(66307..66915)
                     /gene="hisH"
                     /locus_tag="PK589_03335"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNJ18657.1"
                     /translation="MIAIIDYGMGNLRSVSKAFEAVGHQAVVTREARVIADASHVVLP
                     GVGAFGDCMANLERYELIGPIHAAIQSGKPFLGICLGLQLLFAESEEFGTHKGLGIIP
                     GRVKRFVLDASLKVPHMGWNDIRIMRPAPIFAGIAPGSYCYFVHSYYVEPADSAVVAT
                     VTDYGAPFASAVWKDNVVACQFHPEKSQAVGLQVIKNFGAWT"
     gene            complement(66980..67585)
                     /gene="hisB"
                     /locus_tag="PK589_03340"
     CDS             complement(66980..67585)
                     /gene="hisB"
                     /locus_tag="PK589_03340"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNJ18658.1"
                     /translation="MKNTGVPRQASLHRATKETDIRVEWTLDGTGQGKIDTSIRFFDH
                     MLDLLAKHGFFDLTVQAKGDIDIDEHHTVEDVGIVMGKALHQALGEKAGIKRFGWASV
                     PLDETLAQVTVDLSGRPYLVYNVNLPDRKIKTFDLGLFEDFFQAFVTHGGLNLHINLQ
                     YGRNPHHIMEAIFKALARALDQATLPEERLSGAVLSTKGSL"
     gene            complement(67582..68865)
                     /gene="hisD"
                     /locus_tag="PK589_03345"
     CDS             complement(67582..68865)
                     /gene="hisD"
                     /locus_tag="PK589_03345"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNJ18659.1"
                     /translation="MTILESTDRAFAKALRKITARSRSQAATVEKSVRAILQAVEKGG
                     DRAVLRYTKKFDRVSVTLDQIRVTPEEIKEAYYHIRKDEGDSLRYAAERVRQFHERQR
                     TKTWMYQEENATLGQMVTSLDAVGVYVPGGKAVYPSSVLMCAIPAKVAGVRRIVMCTP
                     TPKGEINPYLLVAADIAGVDEIYRVGGVQAIGAMAFGTKSIAKVDKIVGPGNMYVATA
                     KRLLYGTVGIDMVAGPSELLVVADDDAKPAHVAADLLCEAEHDEDAQVYLVTTSASLA
                     KEVVRLIQVQLKGLQREKIAAKSIARHFVAFVVPTMDEAIEVANDIAPEHLTLSVDRP
                     FDYLEQIRHAGALFLGRYTPPAVADYVAGPNHVLPTGATARFFSPLSVNDYVKVSNIV
                     HYTREELTKAKDHIVRLAHIEGFDAHAKSAQSRFS"
     gene            complement(68951..69619)
                     /gene="hisG"
                     /locus_tag="PK589_03350"
     CDS             complement(68951..69619)
                     /gene="hisG"
                     /locus_tag="PK589_03350"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNJ18660.1"
                     /translation="MKARKSQDEKPKGLTIALSKGKLLGPVLHLMKAAGYYSPGLSVE
                     SRKLVFDCAANNATFLIVRPTDVPTYVEHGAADVGVVGKDVLLEQGSDVYEPLDLKVG
                     ACRISVAALQGQPSPDRWSSKVRVATKYPNVTERYFNQRGMPVEIVKLYGSIELAPIV
                     GLADRIVDLVETGSTLKAHDLAEVEVITHSTARLIVNRASLKLKHEPLQVLIDRLRTA
                     VAAE"
     gene            complement(69622..70878)
                     /gene="murA"
                     /locus_tag="PK589_03355"
     CDS             complement(69622..70878)
                     /gene="murA"
                     /locus_tag="PK589_03355"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNJ18661.1"
                     /translation="MDQILIQGGRRLQGEVRTSGAKNAALPILASTILGGGECVLSNM
                     PRVVDVLTMGKLLRMLGIAVSQEGDQTVVQAEAIASTEAPYDLVRTMRASVLVLGPLV
                     ARLGEAKVSLPGGCAIGSRPVNFHLAGLEKMGAAVEIEHGYIKASARRLRGSRIYFDT
                     PSVTGTENLMMAAVLAEGTTVLENAAKEPEISDLAAFLVKRGARIAGAGTDMITIEGV
                     TELHGADHEVIPDRIEAGTYLVAGAITGGEVIVDRCRPEHMEPLLMKLRESGAELIEE
                     KERVHLKVTGRLKGTNVRTSPHPGFPTDMQAQFVALMAVAEGTSVVTETIFESRFMHV
                     EELRRMGADIRVEGNRVVITGKERLTGAPVMASDLRASAGLIVAGLAAEGTTEVSRVY
                     HLDRGYERLEEKLRILGASIERRKGK"
     gene            complement(70922..71848)
                     /gene="prmC"
                     /locus_tag="PK589_03360"
     CDS             complement(70922..71848)
                     /gene="prmC"
                     /locus_tag="PK589_03360"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNJ18662.1"
                     /translation="MRQQQLADAACSVSSLGELLRKSESRLDAAGIDQPALESAWLVE
                     HVLKLSPLMQRINTERGLTPEECEGVQVLVERRANREPLQYVLGTQEFCGRDFFVTPS
                     VLIPRPESVLLVEEAVRRCVQISDATIVDVGTGSGCLAVSVASALPTARVLAIDASTD
                     ALVVARNNMARYGVEARITCLQGDLLDPIGQEGLPNQVEVILSNPPYIPDGDVETLQP
                     EVRDFEPRLALAGGHDGMDVHRRLIAQAPAYLKPGGALLMEVGIGQAAAVCRHAEEGG
                     RFRTYVVLRDQGGIERVVCFEKKEESLSSSSS"
     gene            complement(71845..72927)
                     /gene="prfA"
                     /locus_tag="PK589_03365"
     CDS             complement(71845..72927)
                     /gene="prfA"
                     /locus_tag="PK589_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNJ18663.1"
                     /translation="MEDGLIKKWEAMAARFQELTNQLMDPTVLNQPGMIHKLNKERTE
                     LEEPAQEFAAYSQAQKQLHEVTVMLQDSGTGADMKQLFAEESEHLTQQLLDIEARAKD
                     FLTPKDPRDEKNVFLEIRAGTGGDEAALFAGDLFRMYLKYAERNRLRLEVVDASETGI
                     GGYREVIALLEGKGAYGRLRYESGVHRVQRVPTTEASGRIHTSTVTVAVMPEVDEVDV
                     HIDPKDLRIDTFCSSGAGGQSVNTTYSAVRITHIPTGVVVSCQDERSQLKNRTKAMRT
                     LRARIVEAEREKQEAAIAQDRKSQVGTGDRSEKIRTYNFPQNRVTDHRVGLTVHKLDQ
                     VLAGDLEEIVQALRAHYEHLASLETK"
     gene            complement(73021..73227)
                     /gene="rpmE"
                     /locus_tag="PK589_03370"
     CDS             complement(73021..73227)
                     /gene="rpmE"
                     /locus_tag="PK589_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNJ18664.1"
                     /translation="MQKGIHPVYREATVHCACGNTFKTRSTIGDIKVDICAGCHPFFT
                     GTQKIVDTEGRVERFKKKYAKKDK"
     gene            complement(73321..74568)
                     /gene="rho"
                     /locus_tag="PK589_03375"
     CDS             complement(73321..74568)
                     /gene="rho"
                     /locus_tag="PK589_03375"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017631298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNJ18665.1"
                     /translation="MHLAELKQKSIADLNDVARDLKIEGAANLRKQELIFAILQAQTE
                     KNGVVFGEGVLETLPDGFGFLRAPDSNYLPGPDDIYISPSQIRRFNLRTGDIVSGQIR
                     PPKESERYFALLKVEKVNYEDPEVARDKILFDNLTPLYPEERLNLEFDREEYCTRVMD
                     LTTPIGKGQRGLIVAAPRTGKTMLLQAIARAILKNHKEVTLIVLLIDERPEEVTDWQR
                     QVKAEVISSTFDEPAQRHAQVAEMVLEKAKRLVEHKKDVVILLDSITRLARAYNTIAP
                     PSGKVLSGGLDSNALQRPKRFFGAARNIENGGSLTIMATALIDTGSRMDDVIFEEFKG
                     TGNMEVHLDRRLADKRLFPAIDISQSGTRKEELLVDRDRLNKMWILRKVLSPLGTMEA
                     MEFLMDKIGGTKTNQEFLQSMNR"
BASE COUNT        16319 a        22925 c        19157 g        16072 t
ORIGIN      
        1 acatagagag gcatagcgca ggggtgctgc gggcgaggtc aatcgcacgc tcaaccagag
       61 cggaagcgca tacaacagcc aaatgacatg ccctagcgac atcgcgacat ccagggcaaa
      121 gacaccggca gtcagcaggc agatggcaac caacatgctc gaatcgctcc caccagactc
      181 acatcgacac cgattcgcga ttcacaggct aaaacatgcg taagataccc cccagaacaa
      241 atcaggtaaa cccctgacaa tcggccctat cacggccctg cctgaatgga tcaggaccat
      301 ggattggtcg acggcaacgt agccccaggg gaggaatgtt tcatcgcgaa acagactggc
      361 tctaaggggg aaaaccgaaa ctttcgcgat gaactgatgg aagtggcgga tggcacgagg
      421 gaaaaagaga gggtcaggtc gtcgctaggc cctagtcagg aggttctttc aaatcgccaa
      481 ctgtggtcga cgaacatgcc ttttccccgc catccgtcat gcggatacat tcggccggtc
      541 ccgtaatagt cgccatctaa gacctgcagc tcagctgcat actgaaccca atcctgcgtc
      601 atggtattgt tttcaatcgc ggcatggaga aagtatcggc cgccgcataa cggcagatgc
      661 ggcacatgga agtccacata gccgctgccc tccaaatcca gaggggtatg actcacaaga
      721 tccgtcgaaa aaatacagga cgtgcgctca tcacgattga tgacaaccat gacacgcata
      781 tccttaaatg ccttccggac atgacactta tactgcacgc gaatgacaag atgttgcccc
      841 gataatgccg gctgcagagg gttaagtttg tcgtccagga aggtaatcgc ctgcaccgtc
      901 agttcgcctt tggtgtaacg atcggtgcga tcggctagcg agactccggt acctgtgaat
      961 gcctcgctca tatattgctc aatgatctgt tgcggttggc catcacccac gacccggcca
     1021 tgccgcagca ggatagcccg ctgacagagg ctcgccacta ccggcaaatc gtgactgacg
     1081 agaatgaccg tccgtccgtc tctccgtacc tcgtccattt tccccaaaca ctttttctga
     1141 aaagcaccgt cgccgacgga gagcacttca tcgacgatca gaatctctgg atcaagatgc
     1201 gctgccactg aaaaagccag tcgcacatac ataccgcttg aatagcgttt gacaggcgtg
     1261 tcaataaatt gctgcgtccc ggagaattcg acaatggcct cgaatctctt attgatgtcc
     1321 accttgcgca tgcccagcat agcggcattg aggtacacat tttctcggcc cgtaagctct
     1381 gcatgaaatc cggtgcccac ctccagtaat gatgtgacgc gcccgtatag atccacttct
     1441 ccaccggtcg gttcggtaat gcgggacaaa atcttcagca gcgtgctttt ccctgccccg
     1501 ttatgcccga ttaccccgag gacctcccct tccatgacat tgaatgagac atcgttcaat
     1561 gcctggaagg attttggggg tacactcttc gcagcgcgtc gtcccaggag agaccgacag
     1621 ccatgagcga tgcgatcccg caaagtgtca tgattgatct ctgcgatgac atattctttg
     1681 ctcacgtgcc gtgcggaaat tgcgatctca gccatcaaat cacgtccgcg aaagtgcgtt
     1741 ccatacgttt aaagtaaacg atcccgctca cgaacaaaac caggacgatg cctacgctcg
     1801 ggagaatcgt ttcgagtgag ggaggacgtt gccccagcaa gctccatcga aaaccctcta
     1861 cgactcccac cgcgggattc aggctgtaga gggtgcgcca tttttcagga atgagactcg
     1921 cgggatagac gactggcgaa gcaaacatcc aaaactgcac gaaaaatggg atgatatgtc
     1981 cgacatcgcg aaatttcaca tgtaacgcag aaaaccagaa actcacagcc agggcggcga
     2041 gcaaggcaat tcccacaaaa acagggaaga acagcaactg ccaacccggg gtcacgccaa
     2101 accataacat gagcggaatc atcatcagca acgcgcagag cagatcaacg gcgggtgcag
     2161 atgcggcggc gagcggaata atgagacgag ggaaatagac cttgctgatc aaatgcgact
     2221 ctcccactaa actggtgccg ctgcggctcg tcgcctgagc gatgaagttc cacggaagta
     2281 ggccggcata ggcgaataat ggatagggga cgccatcaga cggcaacttc gcgaggtaac
     2341 tgaacagcac agtgaacagc agcatggaga tgagtggctg gaacaccgcc catcccgcgc
     2401 cgatcactgc ctgtttgtaa cggacttttg tgtcccgcca gattagaaag agcagcaatt
     2461 ctcgatactg ccagacagac tgcaggtcta gatgaaacag acctcgactg gggcgaattt
     2521 tgaccgtggg cgttcgaacc gccggcgcga gagtgccagc cgtcaacggt gacgcctgct
     2581 gcaattccga ctcattcata agtatgacga ttccatcaag gcttgatgag atatggctcc
     2641 caaaaggagc gatcatagcg tggccatgca tgacaaccga agggcaatcg attctgattc
     2701 agctcgaaac aatgccgtgg gactacttca aaggcaaaat ccaatccgac atgaaacggc
     2761 gccaccttga attgggcatc atagcgcacc gcttcgtcgg cccaaaaatg atcttcgttt
     2821 ttggtcccgt caggacgcag atgccaacgt tccaattccc gcttcacgtt attgaatcgc
     2881 ttgatttgct tataccattt tctcggcaaa ttgacagatt tcacgtacca ggactgtccg
     2941 gtagtaatac gttgccaata cacctcagga tccacccaat aggcgtccga ggacaggaca
     3001 cgcaaaaaac tggacacctt tcggagagag aaaccgccgt tgccgacacg agacgcgccg
     3061 acccacggac tatcggcaca cttcagccaa ggggcaccga cgtagtccca atcacttgcg
     3121 caccactcca tcaaccgatc ggaaaatacc aacgcgtcga gctgatagat caacacatat
     3181 cggtacgatt gaaatgcgcc ataaaacgtt ggggagagca tcaatctcgc attcgcgatg
     3241 gcacttccaa aataggaatc gggaaatcgt tgaatgtgaa acccaggccg ttcaatggca
     3301 aggctttgag gaaccacgag gaatttgtca tagcgcccga ggtaatgctc taaatgccgg
     3361 aaggaaatct cttcatcttc ggtaaattcc gccctgttat atccaggcac caccaccgcc
     3421 acactactca tccctatgcc tctttcttga gctcccgtca ttgtcttcgc gtttcaggcc
     3481 gttgccagcg atggcttaaa ccaaaaggag cgtcttggcc gtccggattg aagcagctcc
     3541 ggcaccgtga caaaccgata gcttccgctc aatcgatcaa gcatcgtctc caaggcgtcg
     3601 agcatggctt gacggtctgg ccacgatgta tgttcggaat cggtcccatg agcaggcgta
     3661 cctttatcgt acagagtatc gtggaagagg acgatcccgc ccggcgacac catttccaac
     3721 agatatttgc ccattaccga ggactgggat tcataccaat ctccgctgtt cagattccat
     3781 ccgacgacct cgtaccccat catgaacgct tccaggcgtg acatgacggt ctgctctcca
     3841 taaggcggac gaaacaatgg gctgacatag ggccgcaatg cgcgctcgca ttcacggatt
     3901 tgccggcgac gctcccggct ggttaaaccg ggaaatgcgc ggtgatccca ggagtgaata
     3961 ccgatagcat gccctcccgc agctatccgc tgaacaaggg ccggctgaac tgcagcagct
     4021 tgccccacca caaaaaacgt cccacgcgcc tgatactttt caagaagagc caagactttg
     4081 ggggtgtttt cgggatccgg accgtcatca aaggtcagag caagcactgc gctgggcgtg
     4141 acgacatggg ttagacttcc caacaccttc cgcactgagg ttgacagaaa ggattgaaga
     4201 ctcatggaaa cgattcccgc gtcattccac catcactggc ggggcccaga atcggaggct
     4261 tccctttagc aggcccatca aacgtccaag acgccaggcg aatagggtct tcccttccgg
     4321 agtacgaagc agttccgaca tacgtccgag cagattacgc cacgcctgta tgtacttgcg
     4381 ccaacgccac cattcccaca gatgaccagc cccatacttc ttatataagt agacttcata
     4441 ttccgcatac tgcgaggcct gggcatagac gccttttttg ctggcccgaa gccggcaatg
     4501 caataccgcg tccggaacaa attcgagttt tatcccttgt tgctgcactc gcatacagta
     4561 gtcgatatct tcacaaatcg gaattgtttc atcaaatccg gccactgcat catgcagggc
     4621 acgtgtgata ccaagtgttc ccgatcctgc gtgcggaaat ggcaaaaatc gaaactgttg
     4681 gagcccaacg atctgcgtgc agccacgcgc cgaccgaaca accggactgt tgagcttttc
     4741 aaactccaaa cgagaggcca caaatccgtg catgcgtacg gctttttcca tggcgatcgc
     4801 ccaacctgcc gccggaacat catccgcatc acaaaacaaa atatactccc cggtcgctgc
     4861 gttaacccct atatttcttg cgtaggaagc gccccgccgt gcagaggcgt caacaagacg
     4921 aagatgcggt atccgcaagg tgtacttcgc gactactctc gccaggttgt cggtggaacc
     4981 attatccgcg acaatgactt cccacgctgc cggccacttc tggtcagcaa gcgcctgcag
     5041 cgtctcctcg attgtgcgag cagcattacg gcacggaatg atgatactga gtgtcatggt
     5101 tcctcggacg tcaaagtcct cggcgaatac ggctacggag agactttcgc gtgttctgct
     5161 gtggctaagg attctcgcgt ccaagcgcgg tacagcgaat aaagacgtgc gggctgcatc
     5221 caggagctag gatcattcag cagggcgctg actgctacaa gaggggcgcg tcttggagaa
     5281 ccacgcttca tactgatcgc cgccgcctca agaagttctc cgaataatcg acgcaatgaa
     5341 ccggtgtaaa ttgtctccgc ctctgcgact gcgtttgtga tttctttcca gaacttcttt
     5401 acagccgcga ctggatggga tgaagaccct gccagggtgc tggcgatacg cttcaaccat
     5461 tcaaccaccg ctgcttcttg tagtaaccaa ggagcttcgc gcacggcnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnntca accattcaac caccgctgct tcttgtagta
     5641 accaaggagc ttcgcgcacg gcctttaaca aatgagcttt ggtcggctcc agaccgccac
     5701 cagcccacca gcgctgatgg gcaatactgg cacaaacggc cgctctcgct ttccggcgga
     5761 cacgatcagc atccgctgtg gcaggcaggc gcaatagggc agcttctata atcttaggca
     5821 attgctgctc atttgttcca ttcacgatgg tgctgtacca cagcccttgc tgagaataac
     5881 gtccgcgcgc gacgggtccg ccggagagaa acatccatgg agtcagcgcg gcaatacgaa
     5941 gcgccatatc ataatgttcc attccggcct tgctctcatc gaagaacccc gcctgttcaa
     6001 ataactcgcg tcgcaccaac caggtcggtg gatggagaaa atcgtcggtt gtcgtgacaa
     6061 actcctcaaa cacgttgcct gacggccccc attgaggcca cagcaacagc ggcgtttctc
     6121 cccctgtcac cacgctttgc ccatacagca cgccgatttc cggatgcgcc tccaaaatcg
     6181 gaacctgcac catcaattta tgggtgagga attcgtcatc atcgtccaaa agagcgatgt
     6241 atttcccttt tgcacgctta atccctgcat ttcgagcgcc ggacgcaccg cgattctgat
     6301 ccagtctgag atattgcact cgagggaatt ctgcgacaac ctttgccgtc tcatcgggcg
     6361 aacaatcgtc aactacgata atctccatct caaaaagctc cccggcttgc tcttgtgcca
     6421 aggcggatat aatagctttt ctgagaagat gcgcgcgttt gtaggtcggg attaccacac
     6481 tgacgagcgg acgttccgga gaaggacagg gcattgcatt cacgcctcct tcttggcgtg
     6541 aaagtgactc gttattcata gagacgctca cctctctgag tctcggcacc accctgatgc
     6601 gacattgcct gtctagccat tatactagta agcgggaggc acgaacattt atagtaaata
     6661 ttcgtgcttg attgagggta atgcgtgcgt cagtcgtcaa gagacaggca gtagacagag
     6721 ttctcaaccg atctgcgttc actcgccagg cacattgcac cgaacgccgg attccagagg
     6781 aatccattgc ggtcggcaag cagattcttg cactgcttct attgtccgct gtacagcgag
     6841 gccgtcgtaa aaggacgcat cgacgtcatg attcaatctt tttcgtaaac aggcatccac
     6901 aaaacttcgc atcatcgctc cgagacaatg gggcttgccg tcggaggccg ccgccggtag
     6961 cggcacctgt tcccaccctg gatgtgtaga gcgtgacacc ctcaacctat ctacggaacc
     7021 acggctcaga gaggcccgga gcgcgccttc tcgtcccacc acctcgatat acgctttatc
     7081 tccagaattt ggggtggcac gactggcgaa gagatgacct tgaatcccat cccggcatac
     7141 aaaccatgcc gaagcaatat catcggtctc tacctgaccc agcactccgg tcctgtgatc
     7201 aatgcgttcg cgggggatca ataaggtact acccgccacc gcctcgatcg ggccaaggat
     7261 aaaccctact agatcgaaaa gatgagatcc gacatcgtac aaaattccgg cacccgcgca
     7321 gtcgcgtttc tctcgaaaac cgattttgga atccggcttc acaccttccc aggacgcata
     7381 ttgcacacgc acatgatacg gttcgcctat gtcgccttta agcagccgac atttcaattc
     7441 ttgaacacca tacaaatacc ggtaggtaaa tgcgacttgg tggatggcat ggctacgttc
     7501 tgcggctcgg accatttcaa tgacttcttg aacattcatg ccgagcggct tttcacaaaa
     7561 gacatgcttc ccggctgaaa acgcagcctt tgcctggagg gcatgcgcca cgtttggagt
     7621 tgcgatggtt accgcatcga tatcatttcg tgcgcagacc tgttcgtagt cgagcgaaat
     7681 ttccggcacc ccgagacggt cggccatggc tcgagttcgc gccgagtttc gcccgcataa
     7741 gacgaccacc tctgcgccgg gatgcgattg caatcctggc acgtgacacg cttcagcaaa
     7801 tgcccctgtg ccaaggacag ccacgcgcaa ttttttcata gaataacctg atgaggaagg
     7861 accagcagcc caatttggta tggcgaatgt tcagccatga ttcaaggaac gaacccccgt
     7921 gaagcagcgg taatatggca caccacatcc cggggtgtaa taggggattc cctgagtcct
     7981 cgaaattatt ccggatcgca aacggaacaa acgcggcagc aaaatcaatc taacagattg
     8041 ttattcctca cgtatctgaa cgcaccaatc ctgtccttat ggccaatctc actagcccgg
     8101 gaacatcgtg gatttcgagg cgttccatca gttgggctcg atgggtttcc acagttttga
     8161 cgctcaaatc aaggcggtgg gcaatctgtt tggtcgagca cccctcggca atcaattgaa
     8221 ggatttcacg ttgccgcccc gtgagtttgc tcagcgggct catcggcaca tcatcctgcc
     8281 gacagtaggc atccagggtg aatttagcca tagccggagt caaataagtc tcgcctcgac
     8341 agacggtctt aatagccagc tctaattcgg gacgatccgc atctttgagc agataccccg
     8401 atgcgccggc tcgtaaggct tccttgaggt actcctcatt cgtgtacatg gtcagcatga
     8461 gcactttagt gcggggacat tcctgccgca tccgactcgt cgcttccaga ccattcatgc
     8521 cggacatggc gatgtccatc aacaccactt cgggatccag ctctttcgca acccgcagtg
     8581 cgtcgcgacc atcgctcact tcaccgataa cctgaattcc ttccagtttc tccaggagag
     8641 cccgaaatcc cgcccggacc aatgtatgat cctcagccag caataccctg attttgttca
     8701 taccaatgaa tcctcagtgc ctttggtcgc gacaccatgc ggaaagtacg cacgaatctc
     8761 cgtgcccacg gccggagttg attcaatgct gaacgctccc ccgacaagtt gaacccgctc
     8821 ttccataccc gagagtccga cgctggctcc ggatcggacc tcatcccgat caaacccgat
     8881 tccattgtcg cgaatcacca tcgtcagcgc ttgggaagag cgctccagca gcacttctac
     8941 cttcttcgcc tgggaatgac gcacgacatt cgtcaacgct tcttgcgcaa gccgaaagca
     9001 ggcaatttca atttccgcag gcggcctgct ggtcaggccg tcagcagaaa acgtcaattg
     9061 ccagcctgcg cgttcggctt gtctgccgac gtaccatctg agagccggca ccaaacccaa
     9121 ttcatccagc aaagatggcc gtagatctaa cgccaaacta cgaacccgct gcagcacctg
     9181 agccaatatt tccaaactgt ctgcgatctg atcctcgaca ggctcgcaat tgggatgggt
     9241 tcgaagctcc cgaagattca acttaatggc cgtcaacgct tgaccgatct cgtcgtgcag
     9301 atcgcgggcg atcgcccggc gctccaattc ctgaatctcc atgagacggc gcgaaaggat
     9361 ccgtagccgc tcattagcat cacggaggtc ggttgtacgc tccatgaccc gcaattccaa
     9421 ttcttcatgc gcatccttca aggctttctg tgcccgtttt tgctccgtga tatccgtgtt
     9481 caattccaga ctgccgctcg gcatcccagc agcatcgcgc aacagcgtcc accgactgga
     9541 gacggtaatc tccctcccgg cacgtgtctt atgcacgagt tcaccgcgcc acagcccctg
     9601 ctccagaaat tgctgtttga tctcctctaa cggaagagga aaggtggtgc tcaaaaagtc
     9661 atggatatac gcccccagcg cttcttcagc cgtccatcca tacaatcgca cggcgccttc
     9721 attccaatac gtaatcgtat cggcttgcag atcgcgaacc agaatggcat cgttggccaa
     9781 gtcgagcagt tttgcctgac gacgaagcag tgcctcggct tgcagttgcc cgcgtcgatc
     9841 cttggcgaca aagcagtacc ccagaacgtg tccagcttca tcaggaatgg cactcacgga
     9901 gagaaatacc ggaaaccgat atccgctccg atcaagatac gtccactcgt cttcggtcat
     9961 ccgtccaata gcgacccggg caacgaggac accgaacgaa tcttgaattg gctttcctaa
    10021 gccggccgca agggcttctc tgcggcggcg tagctcatcc ggatcatgaa aggcagccgg
    10081 cgtcacttta ccgacaaccg catcggcgcc gactccgagt aatcgctcgg ccgcgctatt
    10141 gaaactggta atgattccgt ccggccgagt cgagatgacg agcactctgc ttaagccggt
    10201 atcatgatgt tcaatcatgt ggcttcccgt ggggacccat cccgataaga ctgagaggag
    10261 aatgatgggc tggctgccaa cgttccagcg atcgtggagg tgccaacctg gcggcaatca
    10321 tactacagag cgagcattca agcgacagcc ggaacgaaaa gagcgtcaga gcagaacggc
    10381 tggatcgatg acaccaacga gcagcgcacc gagctagcca cgacgttttg agggctcggt
    10441 ttgtcggccg accaaccaca cgcccatcag aatgaggaca atgcccgcaa tttcccgtgc
    10501 cccgattgct tcacccaaaa tcacggcgga gagcaacaat gccgatacgg gaatgagatt
    10561 cacgaacacc cctgcccgcg aaggtcctac gccttgaacg ccatataacc atgcctgttg
    10621 gcccaacgcc gtggcgaaca caatgagata ccccacggcc agccaaccgg aaaccggcac
    10681 agcgcttgta ccggtcacca acattttctg atcggtcgag aggagaggaa tctgcaacaa
    10741 cagcgagagc agcagcgtgg tccagttgac ggtcagtgga gacaatcggt tcatcacctg
    10801 acggcccccg atggaataca gcgcccagct caccagcccc aacaatacta acgtcccacc
    10861 taaccaggga tgatcgccga tgggagcatc ccctccgact cccgaaacga gcgcgacgcc
    10921 agcaaaggag accgcgcagc cgcctgcgac ctggttgaat ggaatatcac gaaccaagag
    10981 ggaagacaga agcgctgtaa tggccggact ggcgccgatc agaaccccag ccgttgaggc
    11041 tccgatatag cgaaggccga acagggtcaa cagatggtta ccgaatacga acaaccccaa
    11101 taaagagagg actttgaggt cgcgggccgt taaagttgta cggttacctt cagtccacca
    11161 ccacaacggg attaagatcg ccagcgcgcc gatgccgcgg atgagagacg tctctacggc
    11221 tgaaaacgca ccaagagcca gcttttgccc gacgattgaa ccaccccaga ccaaggccga
    11281 aatcgtcaat gccgcatagg cggcggatgc ggacggtttt tccattcaag cgattcctgg
    11341 gatttatatc gagtggtcga tcggaacgtc tcgaccagtg atgctagtgc gacgatttgt
    11401 gagtgatgac ccagatcccg tcgcgaatgg tcgcgagaac ggccgtgaca cgcggatcag
    11461 ccgccacgat ccggttgagg tcctggattg ccgcagtccg gtcatccggc ggcggatcgg
    11521 tgaggacatc ccccccccac aacacattgt cgaccaggat gaccccgcgc ggcgacagca
    11581 attccatcgc tcgccggtaa tagttcacat agttcagctt gtcggcatcg ataaaaatca
    11641 gatcgaaggg tcccgtcaat tctgccatgg tctcgagtgc cggccccata cgaatctcaa
    11701 ttcgacggcc atgcggcgac tgcgcaaaga atcgacgcgc taccgcagca gagtcctcat
    11761 ccacctcaca tgtgatcacc cgaccctgat cgggcaatgc ttcggcaaag cagagagcgc
    11821 tgtagcccgt gaacatcccg atttccagga cccgctcagc tcgcaccagg cgcgtcatca
    11881 catgcagaaa cgccccttcc agcgggccca ccaacatccg ggcgtactcc attgtacgtt
    11941 cagtttcctc acggagcgcc cgacgaaccg gcgtttccgg gagagaacag gcagcagcat
    12001 aggcatcgat attcggcgac acgagatcgg ccatcagttt cactccagga gacagttgtg
    12061 cgacaattcg caccggccgc gaatcatacc acggacatcg tggagaaaga gcgcacaagt
    12121 gaagaccttg aagaccttgg aaccgttaac gaaccgactg ctggaaatcc gccggattca
    12181 aagcgcagct tctgttctgt cgtgggatca ggaaacatac atgccggctg gtggcggggc
    12241 agctcgcgcc gaacaaattg ccacgctgga gggtctggcg catgagcggc ttgtgtcacc
    12301 tcacattgaa tccctattga atgagtggat cgacccttcc accgggcaag cggcagaaca
    12361 ctgggatgaa ccgtcgcgat cgctcttgcg ggaaacctgg cgtgatttca gtcgtgcgag
    12421 aaagctcccg tctgattttg tcatccgtct gagccgtgaa tgttcgttgg cgcaacaggc
    12481 ctgggtgacg gcacgagcag aaagtcaatt ctcgaagttc cttccttcgt taagaaccat
    12541 cctcgatctc aaatgtgaag agactcagta cttgggatac cgggactcac cctatgacgc
    12601 gctactggat acctatgagc caggcgccac agtgggccaa ctcacgccgc tcttcgcaca
    12661 acttcgggcg cggctggtcc cgctcctgaa acgagttcag gccagcaagg tgcgcatcga
    12721 cgacacctgc cttcaccata gctttgatca cacgaagcag atcgagttcg ggcgactcgt
    12781 ccttgtcgcc atgggatatg acttcgagcg tggccgcctt gatctctcgg cccatccgtt
    12841 tacaacctca tttcacccga cggatgtccg cgtgacgacg cgggtcttcg agaaggattt
    12901 gccgtcatgc ctgttcagct gcatccacga aggcggccat ggcttgtacg atcagggcct
    12961 ggacccgcgc tattacggat cgccgctcgg cgagtctgtc tcgctcggtt ttcacgaaag
    13021 ccaatcgcgg ctctgggaga actgcgtggg ccggtcgcga gccttctggc aatgctttta
    13081 ccccattttg cagcacacat ttccgcagca attggccggg gtgcctctcg atcaattcta
    13141 tgccgcgatt aaccgcgtca ccccctcgct catccgcgta gaagccgatg agctgaccta
    13201 caatctgcac atcatgctgc gcgtggaaat tgagcaggcg ctcattgaaa gacgggcgca
    13261 tccggaagac ctgccaagcc tgtggaatga cagaatggag gcctacctgg ggatcacgcc
    13321 ggcacaggac gccgacggcg tccttcagga cgtccattgg tcgatgggag ccttcgggta
    13381 ttttcccacc tacaccttgg gcaacctcta ctcggtgcaa ttcttcgaac aagcaaagct
    13441 ggagctcccg caattggaag aacagatcgc cgccgggcag ttggtgccgc ttcggcactg
    13501 gctggaacaa aagattcacc ggtgggggcg tatgtttacg ccggaccatt tagctcgacg
    13561 tgtcacgggg aacggcgtga cgccagaacc gttcttacgg tatctggagg cgaaatacgg
    13621 agccctttac cgcctgtaat cctccccagc agtctcttcc cgtctctctg cagatgagtg
    13681 ccacgcgggc tcgttcggga atacaagcat actggagtcg cgagaattct ctagggccat
    13741 aacagggccc tttagctctg gcgcccgccc cttacgcgtg atacttttcc cctacttaaa
    13801 tctaggaatt tttagagctt tccctgagat tgaatcgact gagcacgtgg gccaaggaat
    13861 cgaaggaagt ctcagccagc caatttgcgc cggagaagtc ccggctgtag cctgagggtc
    13921 gatgtccgct ggaacgccgc cacgactaga cgtcatgaag gggcatgaac gctctctttc
    13981 cgcctgtttc cacgccggca gacctcattt ctgctccgta ttggaatggc aggcgggcca
    14041 ggacggatct tcgtttccgc acggcgcccc catgctcagc accacaccat agggggaatt
    14101 cactgctgtg aatgcatttg acacccttcc cgccgtcaaa acagaactca atatcttgct
    14161 cgccgatgac catgccctcc ttcggcgcgg cctcaaggaa ttcctgcagg attttctcac
    14221 cgaagatgga ttaacgcctc acatcaccga aaccgactcg gcccatagcg cgattgacca
    14281 aattcggacg ggagcttggg atctggtgat tctcgacctg aacttaccag acatgcctgg
    14341 attggaagtc ttgcgcattg tgaaatctct tcaacccagt ctgccgattc tgatcgtgag
    14401 catatacgcc gagcaacact atgccgctcg agccattcga gcaggtgcac tgggatactt
    14461 gaccaaagac acgggtccgc aacaacttcg tgctgccgtg tcccgaattc tgcaaggcgg
    14521 gaactatttc cccccgccgc aatcggaaca ggtctccgac acgatgctgg accgcaacac
    14581 accgtgcgcc cacgacctgc ctgcagtcct atcagaccgc gagattgagg tcctccagtg
    14641 gattgcgcaa ggccggcgcc tgaccgagat tgctgaacac ctcaacctga gcattaaaac
    14701 cgtgagcacc tatcgggccc ggttgctcac gaaattggga atgaaaacga ccgcagagtt
    14761 aattcggtat gccctggatc agcaactggt ttaaccccac gaggttatcg agaggaggca
    14821 caccttcacc acaccatctc cgccaaaacg ggcagacatc tgcgacgttt ttttgagtga
    14881 tcgcacgatg atcaatcgct gtccaccatc acttcgcgtc gggggattat ggccaatatc
    14941 ctgattatca acgccgactc gaatctgaaa cggctcatca agttggcgct gcctggaatg
    15001 gagcacttcg ttcagtatag tgccacgtta cacaatgcct ctgctcaacc gcccgcatct
    15061 aagctggatc tcgtcgtcgc ccgccttgtc gccgacgatg accaggggcc ggaagatctg
    15121 gtcgacctac gaaaagcctt tccaagctcc agaatagttg ttacagcggc tctgcacgaa
    15181 cctgccctgg agggggagaa gtttcagcgg gtggtccggg agttacaaat cgagtattgt
    15241 ttgctggaac ccatcgaaac gggagccatg ctgcatacga tacagtcggc tctcacctta
    15301 ccccggcgcc actaaaacca tgtaccagca acgctggtaa tggaagatga gcgggaatat
    15361 cctccttgtt aagagagatc tctaggcctg ctgccagcct tgtgagcaat tccaactatc
    15421 aagcaggtgt gggctcaaga aatcgagctc agtatcccat cgagacattg agcttcgcca
    15481 gatgattggc gggcaagaga aattctgctg gcgcttcaat tcgggacggc accaggagtg
    15541 tggtatgaaa gacgatatcg cggatgggaa ttttgaaatc gggctgctgt ggagtactca
    15601 attccaccga ttcgactgac tgcataatag cagtgacatc ctgcggcccg taggtggcaa
    15661 tgaccgcttg catgaggtcg cgcacctttt cattcgtttc atttccgtca atccctttca
    15721 gcaggagcgg gagcgtctca tcacgaacct gatctgaggg actgaagttt tcgacccgtt
    15781 ccttgcgccg gagcgaggtc aggtcattgt ccaaatcccg actgaacgcg ccatacgcga
    15841 accggacgaa ctcaaagtga aaacccttga gcccctttcc gaacatctga agctcacaaa
    15901 ggaaacagag ctgctgaagc ttcacgtcgc tcaacaaccc atagggctca gccagcgaaa
    15961 gcacatagag cagcagggcc cgatcaacgg tcatttcgtg ggatttgcgc aagggtcgaa
    16021 tctcctcaca attggcggtg acgggggact ataaacaggc cgcttccgat tcgtcaatcg
    16081 acaatggcct actgcgcgtc ccctcttgac cgatcgaggc actttcgttt ttaatgagcc
    16141 tctttcacgc cgagtcacac accatgagca aaaatcttaa agagatggat gtgcttcgcc
    16201 agcatctggc caagcaccaa ttaaagctga ctcgccaacg cgaactgatc ctgaccgcgt
    16261 ttctacgaca ggaacacgtc acggctgaaa ccatgtacca tcagctcgcg aagaaggacc
    16321 cgcatcttgg cctcgccacc atctatcgca cactgaacct gttttgcgag gctggcattg
    16381 cccaggctcg acacttcggt acccagacac aatacgacaa catctctcac aaaggtcatc
    16441 acgatcatct gatctgcacc ggttgcggca ccattgtgga gtttgagaat tgcgaaatcg
    16501 aacgactcca agaggaagtc gccacaaaga atgggtttgt cattcaaacc catcgactag
    16561 aactgtacgg cctctgcgcg cgctgccgcc attgacggat tctcttcaca tccggtatca
    16621 tcttgagacg gcttatcaat ttcaactagg acgccccgag cgtcctcact cgtggaggca
    16681 cgccgcatgg cttcattccc tcgcaccgta tttgctctga ccctgctcat ctcggcgttg
    16741 atactccagg agtggcctgc caagaccgcg gatgcagccg acaaactcgt cgtttactct
    16801 ggtcgtgctg agcgattgat caagccggtt cttgacgaat tccaaaccaa aagcggcatt
    16861 cagatcgaac tcctgtcctc aggaaccacc gagttggtca accggttgca ggcagagggg
    16921 gaccacaccc ctgccgatgt ctttctgacc aacgatgccg gaagcctcga acatgcgcga
    16981 gaactcaaac ttcttcgtcc catgaacatg cgtgaagtcg agcgggccat tccctctcaa
    17041 ttccgcgctt cggacaacag ctggatcggg ctttccggcc gattttgggt ggtcgtctat
    17101 aacacgactc tggtgaaacc cgagcaaatc aagtcgctgc tcgacctggc ccagccacaa
    17161 tggaaagaca aaattgccat cccgaattcc ggcagcgagt accttcaagc cggcgtgtcc
    17221 gtcattaaga ccaccttcgg ggacgaacgc accaaacaat ttctccaagg cctgaagacc
    17281 aatgcggggt cgcaggtcta ccagaaaagt tcacaaattg tggaagcagt cgcaaagggc
    17341 caggtcgcag ccggcatcgt caatcactac tatatttatc gtcatctggc agcacagccg
    17401 accgcgccca tcgccgcgat catgaccgat cagcaggaag gcggcatggg ggccatcatg
    17461 aatgtgaccg gcatcggagt gacccgagcc tcgaaacacg tcgacagcgc caaactatta
    17521 atcgagtttc tggtggccca ggcggggcaa aaactctttg cggacttgga taaagaatat
    17581 ccactgcacc cggacgtaaa agccgacccg gctctcgtcg accggcatag cttccgcgcg
    17641 gcactcgttc ctctagcccg actcgctgaa ttgcgcgaat ccactctcac cctgatcgag
    17701 caggtcggcc ttcgctgacc cctgtggccg agcatgctta ctgcgaccag agttcgggcg
    17761 ccatcttccc tgcagtggat cagtctcttc actgcagcgt tcctggtcct gcccactgcg
    17821 tacattgtct atgtcgcgat gacggccgca ccggccgtat gggctcgcct ctggtccacg
    17881 cgcattccag aactcttagg gaacacgctg tcgttagccc ttggcgtcgc gctgattacg
    17941 ttggtgttag gcgtatcgct ggcctggatc accgttcgat acgacttccc cggtcgccgc
    18001 atatgggaat gggcccttgc gctgcctctc gctatgccca cctacgtgct ggcgtacgtc
    18061 tatgcccacc tactgggaat gggcggcccg acagaacagt ggtggcaact gctcttcggc
    18121 catgaggctc gcctgccctc gccccaaagt tttcccgggg tcaccatgat catggccctc
    18181 gacacctttc ccttcgtgta cctgctggtc cgcagcgcaa tccaaaatct caatgtctcg
    18241 tttgaagaag tcgcgcgcgc ctgcggcgtc tctccatggg ctacgcttcg gcgggtcacg
    18301 ctcccgctta ttcgcccggc cattgcagcg gggttggcac tcgtcatcct gtatgtgatc
    18361 tcagacttcg gtgcggtgtc cttactccgg taccaaaccc tgacctatgc ggtctaccaa
    18421 cagatgacca gccgatatga ccacacggcc gccagtattt tgagcctact gctcgtcgtg
    18481 atggccatca tcttcctggt gaccgaacga tggtttcgcc aacgcagtcg cttctatcag
    18541 acatcgggtc gataccgacg atcgacacgt caacatgcag ggccactcgg aacagcgctc
    18601 atcaccagct acgttgtcct ggtcttcgga gccgcgtttg gcattccggc agccatgttg
    18661 atccaatgga gcgtggaagc catttctcaa ggcgcgttgg acacgcgctt tctgggcttt
    18721 atttggaaca gcagtttcct ggcagcactg gcagcctgcg gcgcggtcat catcggcctt
    18781 ccgttagcct acctcgcgag ccgccgcccc tcacgactga acattgcctg ccttcaagcc
    18841 gcctatgccg gctatgcgct gccgggaccg gtagccgcac tggcagttct cgtacttttt
    18901 acccacttcg caccggcatt gtatgggact gtggtcgtgc tcatcatcgc ctatattctg
    18961 catttccttc cggttggcct ccaatccatg gaaccggccc tgcaacaagt gacacccaat
    19021 gtcgaggaag tcgcccgcac cctgggatgc acgactcacc aaagcctgtg gcgagtcacg
    19081 cttccgctcg tccgcaatgg atttgtggcc gcatgggtgt tgatgtttct tcagaccatg
    19141 aaagaactgc cggccacatt gctgttgcga cctgtaggat ttgatacact ggccatccgc
    19201 gtgtggctgg aagccagcga agagtattac caactggcgg cgcctgccgc attgctcatc
    19261 gtcatcttga gccttccagc gctgatgctg ctggtttcca aagactggcg tggagaggcg
    19321 cagaagggcg tcacgtgagc ctcgtgaacg aatctataac aggccggcaa cagcccgcat
    19381 ccgagggaat aatcccgaat actctggaca cgaccgtcga acgatcggcc gtccttgaat
    19441 tgcgcgacgt atcgtgcgcc tatgagccca cacgaccggc catcgagggc atcaccttca
    19501 cggtccacca aggggagatt ctctgcctcc tcgggccttc cggctgcggc aaaacgacta
    19561 ttcttcgcgc catcgccgga tttgagcggg tgaccagggg tactattgcg ctgtccggtc
    19621 aacgggtctc ctcgccggat accatgattc cgacggagca gcggcatatc ggcatggtgt
    19681 tccaggaata cgccctgttt ccccatttac gggtcgaaaa gaacattgca ttcggcctgc
    19741 gacaccttcc acgaaccgag cagcaggcaa tcgtcgacga cctactcacg ttaaccgggc
    19801 tgcgtggatt ggagcaccgg tatccgcatg aactgtccgg cggtcaacag caacgcgtcg
    19861 ccctcgcccg ggcccttgcc cttcgtccgg tgctgctcct tctcgacgag ccattcagca
    19921 atctcgatcc cgatatggcg agtcgcatgc gccaggatct tcacgccctg ctgagacaaa
    19981 ccaagaccac ggccattctc gtcacgcatg atcacgatga ggcattttcc atggctgacc
    20041 gtgtggcggt cttaaataag gggcgcctgg aacaattcga tacgcccgaa gccatttatc
    20101 acctcccgac cacccccttc gttgccgatt ttgtcggcca ggcggatttt atcgccggag
    20161 tcgtgacgca ccacttggtg acgacagaac tcggcgactt tccgaatact caacacctcg
    20221 cgacgggcac gcatgttgtt gtgatgattc gccccgacga tatccacatc gtcccgacta
    20281 aaggagccga tgcccgtatc cttgcacggc aattcaaagg ttcggagaat gtctacacga
    20341 tccaactccc atcaggccaa attgtacata gtagtgaatc gtcgctgagc atctatcagg
    20401 ttggaaccgc catcgccctg cgcgtggtgg cgacccatac ggtcttgttt ccgcaaccgc
    20461 ccggctctcc gccggtggcg tgacatcaga ctcacttcct gacacgagac cgaacaagac
    20521 ggagaaggac atccgcaacg atgttatgcg gccccccggc gataacagtg gaggaacaac
    20581 gaatggacgt gctgaaaaat gcggtggagt atgggatcat tggcctgttg atcgcgctga
    20641 gcgtgtggtc agtcgcggtc gcagtggagc ggtggctata ttatcgacgc gtcaatctgg
    20701 cgcagtttac cgacgtgcag acctttgaaa tggcgttgac caagcgactc gtcatcatcg
    20761 gcaccgtcgc agccaatgca ccgtatatcg gattgctggg cacggtttta ggaatcatga
    20821 tgacctttca tacgatgggg acctccggaa ccatggccgt gaataccatc atgatcggtc
    20881 tgagcctggc cctaaaggca acagcggtcg gtctgcttgt ggcaattccc tgcgttgtca
    20941 tgaacaatat tcttcgccga cgtgtcgctg aactgctgac gacctataag gtgcaacatg
    21001 gaacgagagg ttaatcaaat caacgtcatt ccgctggtgg atgtgatgtt ggtgctgctc
    21061 gtcatcgtcc tgacgaccgc caccttcatc agcacgggcc aaattcctgt caacctcgcc
    21121 aaggcaaaag aggcggggga tcataaagat gtccccgtcg tcgtcacctt gaccgccaac
    21181 ggagatctgt ttttgaacga ccggcccgtt ccagcggatg gcctcaaaag cgtgatgttg
    21241 acccacccgc gcgaatctct ggtcgtcgtg cgagcggaca aggtcacgct gctggaacgt
    21301 tttgtttcgg ttgtggatga gatccgtggc ctgggcttcc aatctgtcag cctggaggtc
    21361 gtgcgcttgt gaccgctccc cctgccggca tgccggacgc tttcgtccgc ttgcgggcaa
    21421 gcggatggat catctcgctc tgcttgcacg gcgcagcggt cttcttggcc accctgttcg
    21481 ccgccaagat tcgactcccc ccgccatcct cgtcgtttca gtgggacgtg accgtcgtca
    21541 ccccatccgc gtcaactccc acgctcgaca aaccgcaagt tccctcaact cctgcgcgca
    21601 tgacagcgca gagcacgccg acaccctctc cgcgccccgc ggcctccgat gtacaacatt
    21661 cctcgaaatc ggtcccggcc tctcacacta gtatccatga accgctgaca cctacccccg
    21721 ttgttccatc gccgcctcag aatatcgagc cggcacctca acgctctctc gcgcggcctc
    21781 ttcctgaggc agtggaagct ccccgaccgg agccggcaac ggaccattct cccgcgccag
    21841 tggtcccttc cccggcggaa gttcatccac agcaagcacc agtcacttcc ccagccaaag
    21901 atcatgaagt cccagatcca ccaacgtctc cttttccagc tgcacagcaa tccaccgaac
    21961 agcccccagc cccgacctca accgccgcgc tcaccccatc agcgaacaac gtcccggcgc
    22021 ccagaaaggt cgattatggc tggcttgcag gcattctctt gccacgcatt gaagcgctca
    22081 aagagtaccc cgtggatgca cggctcaagc acgtggaagg gagagtggtc gtccgaattg
    22141 tgattcagga agatggccaa atcgtgtccg ccgcggtcgc aaaaagttcc gggcatgaca
    22201 ctcttgatca agccgcactt gaaacagtgc gaaagatttc accgatcgcg ctgacgcagc
    22261 ctctcgaaca atcaccggtc actctccatg tccccatccg ctaccagcta ggaccataac
    22321 ctggctgatg ccggcccttc gttcagcatg cttcccgctc gcctctccga ctgggactca
    22381 cgctgaaggt atgacaacac tagtcgacgc cgtccttgat tcgaactcgt cctttgaggg
    22441 atgaggacac ccagaccttt ccgtcacgat cgacgatcac tgcgccgaca ccaggcaacg
    22501 attccactag cgcgagtccc cgttctgggc ccatcacaaa aatgcccgta tccaacccat
    22561 ctgccgtcag gccgtctttg gcgacaatcg tgacactttg acagtcgcgt gccggctgca
    22621 aggtcaccgg atcaaggaca tgatgatatc ggactccatc ccgctcgaaa tagcgctcgt
    22681 aatcgcccgc cgtagaaatt gcctcattct gaagatctac gaatgccagt actgttcctt
    22741 cgcgacgtgg atggcggata ccgaatggaa aggtcgttcc gtcaggcatt tggccaaatg
    22801 tccggatatc ccccgacaat gccacaaccc ccgccgttgc accggctttt tgcatcgcct
    22861 ccactgccat gtcggccgca aaccctttac cgatgcctcc cacatccacg cgcattcccg
    22921 gttttgcaag atagacggtt ccctgcgcct ggcttaattg caacgcgttc aagttcgtga
    22981 gaggacggat gttctctagc tcagactccg acgggatctg ctggcgatcc aacacactcc
    23041 aagcctcgac cgcggggccg acaagaatgt tgaagccacc gccggtaagc cgagccatcg
    23101 caactgatgc ctgaaggacc ttcatcgtat caggactgac agagacagct tctttacccg
    23161 ctgctccatt cacccgtgac agttcgctat ctttaatcca agtactcaac aattcttcca
    23221 accgatgtat ttcctgaaat cctgccgatg cggcgtattg agctgcctct cggtctgggg
    23281 cgactgccgt aatgctcacc cacgtaccca tatgcatttg ggtgcgcttg acgagaatgg
    23341 gagagcttcc aaggtttcca cctgcgcacc ccagcccaag taacaatagc agccccaggc
    23401 ttccccgaat tgccaaagca aacccgcgcc gtaggacagc atcgtttacg aacatgaata
    23461 gaaatgctcg caaggggtga ctattaatgg acaacttcat cggcatggtc cttggttagt
    23521 gggaatctgc agcgagtcat cttctctgcc actgaggaaa attgcaacct tggcattgtc
    23581 gtgaattccc gcttcaccat gccgcctccc atgtgcctgc ccaatcccta atctcccgct
    23641 aagtcgtccg tttcctcagg atccgatgag ctcagcacac cttcccaaaa gcgtcttgac
    23701 attaccctac tgatcgggat attaataatc gttctcaata ccaggaactc gacacttcac
    23761 tatgacacca accaataacg caatacattc cttgtgccaa gacagcgaaa cacgctgcga
    23821 atgcggtcaa ctgatcgcca aagttcgcgg acaagggctc gagcttaaat gcaagcgctg
    23881 caaacgcatt gtggtcattc cttttacctc aatcgaagga tggggtacgg tgccagcatg
    23941 agtcgattca cgaaaggagg cctcatcacc ggacaatatc cagggcttca accccacact
    24001 cagactttca ttacagacca gagaccattg agtcccgagt cataccataa ccttactcgc
    24061 ggatggagga tccccatgaa gattcgggca atcctcgggg ctctggtcct ggccagttta
    24121 ccggcaatgc cggcgttggc agataacatg accccggagg agatcaagaa gctggttgac
    24181 gaagcggttg aaaagcgatt gcaggagcat gaacgtcgcg aaggtgccgg cgatcacagg
    24241 gaaactgtgg gtgcccctgg taatgagccg ggtatcggct cccttccgga agtcggaaaa
    24301 gaacgtcgaa ccgagaacca agcagcattg tcgttcgggt ccaccggatc tggacgcttg
    24361 gtttatgcca agccgtttgt cgccgcgcca aaagcgatcg tcggtgggta catggacata
    24421 cagtaccgtt cacaccgcaa tggcgttctt gaatcaggca tgggaagcgg gaccaccaac
    24481 ggatttgatc aacaacgatt tgttccattc atctatgccg acatcaccga gcatgtgaaa
    24541 ttcgccgctg aaattgaaat cgagcatggc attcgtgaag atgccacgca gggggccgaa
    24601 atcggcttgg agttcgccca catcgactac ttggtcaaag aaccgttcaa ccttcgcgca
    24661 ggcattcttt tggttccgat cgggaagttc aacctgttgc atgactcgcc gctcaacgat
    24721 ctcaccgatc gaccactcgt cagtcgtctg gtgattccgt cgacgatggc cgaaacgggc
    24781 gccggctttt atggcaccct gtacccgggt cgaaccagca agcttgacta cgaattctat
    24841 gtcaccaccg gcccatgcgg atataacggg gacggaagcc ctcgcattcg tgaagaagac
    24901 ggcactcgac gttcccgaca gcgcaagtgc ccctctgaag atgggttgga tatcaacaac
    24961 ggaaaggcgg tctctggccg cgtcgcgttc agcccgatgc tcggtgtcga agttgccgga
    25021 tccagctact tcggaaatgc ttcccccctg agctacaacc ccctcagcat taccgccatc
    25081 gattggacct tccaacgtgg cccattcgag ctcttgggag aagcggcctg ggcctatgca
    25141 cggggcaatt cccgcgcggt tcagggagcc accttcgcca ctcccggctc gctacttagc
    25201 ggacttaata ccttgaactc atttgccgcc cctccgcagc ggatgcaggg attctatgtc
    25261 caggcgaact atcatttcat gcccgcgttc ctcacggcct tatcaccgaa gcgctttggt
    25321 gagggatcaa cattcaccgc cgtgttcaga tatgaccggg tcaatctcaa tatggacaat
    25381 cgtggagaaa actctggcga attagagcag ttttcgttcg gtctcaatta tcggccgatc
    25441 gaagatgccg tattcaaggc cagctaccaa tacatgccga aatcgtttaa tcccaatact
    25501 ggacagcgca ttcatgacag cgcattcgtg atttctgcag cgacgtattt ctaaattcac
    25561 accccggcgg ctctctctat ggggagggcc gccggattca caagaggcag gatttccgtg
    25621 caaagggcac acatatttat cgcagcagca gcattcctta ccatcggttg cgcctcgctc
    25681 agcggcagtc acgagcagtc ttattcctgc tcacaagacg tcgtctggga cgccgcgatg
    25741 gataccatga aaagctactc gatcacgtcc caggacaaag ccaaaggcgt catcgagacc
    25801 ggctggctgg agatggatgg aaaagagcgc agttatggca ttttcgggcg cacaggattt
    25861 gggaacagag agcgcgcgcg tatgagtctc gccctcaaga catcgaatgg cgctgtctcc
    25921 gtcagtctgc tggaaacacg ccagcgctgg cattcacgag gaggggtcac gtcccaggcc
    25981 actaagtggt ggcccgtgga accatccgaa gaggcgacag cagaggtgac cactcgactg
    26041 aacgaaaaat taaaacaaca ggggtgtacg ccgttatgat gccgttattg ctgctgctca
    26101 ctctcaccgg cgccttcgct ctcgctccac tcaacgccca ggccgagcgt gtatgggaca
    26161 atgagctcaa acgttatttg acagagcagg aaatgtccct ggccgaagtc tttcttactg
    26221 aagaagaagc cgtcaaactc atgtttccga aatcggagcg cgtgagaagg gaactgctga
    26281 accttcccca ggacaagaaa gcattgatcg aaagccgtat cgggtggaaa ttcccggagg
    26341 aatcattcga agtctatatt ggcgagaccg gcacacacat tgacgggtac gccctggtcc
    26401 aaaacaccat cggcaaacac aagcccatga cctatatggt cggagtagat gctcatggcc
    26461 tggtttccaa tgtggaattg ctcacctttc gcgaggcgcg gggcagcgag gtacggacca
    26521 agcgatttaa tgtgcaatat gaaggaaaat ccgttctcga cccagtgcgg attaacaaag
    26581 atatcatcaa catcagcggc gccaccatgt ccgttcgctc gatgacggca gggatcaaac
    26641 gcgttctcgt cctcgtggac gaattttact tgaaacccca agggcggggc agcgacactg
    26701 taaccgctca aaaaacggag aagggcttct tcaagtcgat ttttgggaac tgattttcag
    26761 cagacatgcg taatttcaat acccgttttc gcctccgcga ttcggatcgc catatcaggc
    26821 tcgtctacac attctttctc ctgttgatgc ttgcaggctt cacgttctct tttttctggg
    26881 cacacagcat gactggctta tcgccccaag ggattgctga tcattaccgc ggctccaatg
    26941 ccaccttcgg cgaaccgatg tctttccgag agctggctga aattacccat ttccatctgt
    27001 tcacgatgcc ggtcgtgttt atgatccttg tccatgtcct ctatttgacc aatgccagta
    27061 accgactaaa aatagtcacg acatggcttt ccttcggagg agtcatactc gacctcttat
    27121 cgccttggct gattagttac gtttctcccg tcttcgtctt aaccatgctg acaggcgata
    27181 cattcatgac ggcaggattc ttcatcatgt tcgtcattcc tctccacgag atgtggatct
    27241 tgaaacaacc gttaatggca ggcaaacgcg gagaacacgc atgacctagc attgcggcat
    27301 gtacaccact catgtgcggc cagaggtatt tcgcccagag cccaagacca ttcacatggt
    27361 cttgggctct gacattttgg aggactttca tgcatcgttc gacacgaccg ataccaacca
    27421 caagtcaact tattcaagac ttggcgacgc agctctcagc cctcaatacc ttacagcttt
    27481 ccccggagtc gcttgaggcg gtcagggtga tccaacttga gcgtttggca cgacggatcg
    27541 aaaccgtcat tcccgggcac ttcggacatg gggaacgcac cgcacattat gccttattgc
    27601 tggccgatcg ccttaggctt accaaacatc aacgcctgga tttgcactat gcggcattgc
    27661 tgcatgacgt tgggctcctc atgatgccgg gacatctgct cggtgcgcca gctcctcata
    27721 cacttaacga ctatgccctg atccaaagcc atcctcgcga aggggccgca ctgctgactc
    27781 cctatacatt tcttcaagtg gcggcacggc tgattgccca tcatcatgag cggtgggacg
    27841 gagccggata tccctacggg ctgcgcggcg aatacattcc cgcctcagcg agaattctcg
    27901 cgattgccga tgtgtttgac agcatcacca gccgaaccaa ctcgtgggac agcgccctgc
    27961 gaacgcttca tgcctccgcc ggctctcaat ttgatccggc gttctgcgtc acgttcattg
    28021 agcttctacg caatcacgat cactaccggg cataccagag ctccgatctc tccagcgacg
    28081 ccatgcattc gcaatcgtca ccagaacatg tgccagaagc atacatgaca ggctcaggca
    28141 tctaaccagt cgtatctcca aattactcgt taccgtgggc ggtgtaactc tcgatggagg
    28201 gcatgatgtc aggaaaatcg tctacctcga atatttcatt tgatcggttg cactctttct
    28261 catcagaact tcgagttcaa tcgccacagt gggcggaccg gctggaacag gtcagaacgg
    28321 aacaggactt gggggacctg atatgtgatc tttttaacct ctcccatgcc atcaacccgg
    28381 aaacacagcc tccgcctatc cctcacacgc aaccttcgcg catcgcggcc tttgtaaacg
    28441 agaatctcca tcgtggcgtg acgctgaagg tcttggcgaa tttcctcggc tattctgaaa
    28501 aatattgctc tgatctgttt tgccgcatca tgggcgagcc gttctctaaa tatctccggc
    28561 gacagcgagt cgaacgagcc cgtgcgctcc tcatcacgac cagacagacg ctgacggaaa
    28621 tcgcttcagc gctggggttc agcgatcaat tcgccttcag ccacttcttc aagcgagcaa
    28681 cgggacagtc tcccatggct attcgtttga ctattgctcg acagcagaga caaacgattc
    28741 agggcttgca gcagacttct tgaggcggag ctcaacaagt gatgcggtct gggaatgcag
    28801 ggtgagaata aaagaatggc gggtcagcca gtgtttgagc tcgccgaccc gccatcactt
    28861 ccggttgcgg ttaggacttg tcggcggtga gttgttccgc caggtagcga tcttctccaa
    28921 cctgttcgat cgttcgaagc tgggtctcga accaatcgat gtgttcttcc gtatcgatga
    28981 tcatgtcttc cagccgatgt cgggtggtga aatccccgac tttcgcgcag tgtgcgatgc
    29041 cttgacgaag cgcatctgcg tcctcacgct caaaagctaa atctgccgag aagagctccg
    29101 acactttctt gcccgctgcc acggcatcca gacgtcccat ctccggggat ccgccaaggt
    29161 agaggatatg atcgatcaaa ccggaggaat ggctgacctc ctcctgagcc aaatggctaa
    29221 agtgctcgtg gagtcgagca tagccccaat gcttgcacag agcggcgtgc agcagatact
    29281 ggtgcaccgc cgtgagctca cttaccaaca ccttgttcaa aatatcgaga acgccctctt
    29341 tcgctttcat gcccatgtcc tcactgcacc attccggcgc gggtttaact gtctttcttg
    29401 atctgctcag cgaggtaatt ctcgaggcct atctgcttga tcgtttccat ctgagtctcg
    29461 atccaatcga tatgttcatc cacatccttc gccatatctt cgaacatgtg ccgcgtcgta
    29521 aaatccgcga ccttcgtaca atgcactact ccttcactca atagggcgag catttcctgc
    29581 tcggccttca ggtcgagctt caactgctcg ggaacggtct ctccaatatg gaccgtgttc
    29641 atgcgttgaa cgttgggcac accttccaaa tacaaaatgt ggctgatcag ttcgtccgca
    29701 tccttcatct catcaatact gcgctccctg actttgtgat ggagccgttc atagccccaa
    29761 ttggcgcaca tctttgcatg gacgaaatat tgattgatgg cggtaagatc cgccgtcaac
    29821 actttattca gcagctcaat aacgccttgc tttgccttca tatccccctc ctccggccat
    29881 aacgtctcac ggcctatgga ctagtatctg gtttagtcgc tacgcaattc ctgcttcccc
    29941 attattgtac tccttactta gagattctcg aagggcttgt caaccattga tatacgtatt
    30001 tatgagggcg gttctcaaca tcgccgagaa cgcttctcat tgtcctcatc agaacacgtg
    30061 tcctcgcgcg agcagatcgg tacagtgcag atggagattc ggtcgatcgg gattgaagaa
    30121 caaggaaggt gagaaacaac cttgggacgg gcagaccgtg cgtcgagatt atccggcctc
    30181 gcggtagccg cactcttctt cattacggca ctgcacagag cggccggctt gcttactgac
    30241 tttctcaatg aggaacgggg cctgacactt gggacagggc ttattaatcg gccgatccca
    30301 aagcgcaaac tcgcactccg gataacggct acacgaataa aagtttcgcc cttttttcgt
    30361 gcgcttttga accagatccc caccgcatcc aggctgtggg catttcaccc ccaaggcaat
    30421 ggctttcgtc gttttgcatt cgggataagc ggaacaggct agaaattttc cgaagcggcc
    30481 agatttgacg accatggggc ttgaacattt ctcacacttt tcgtcggtcg tctcctccag
    30541 cttgggaacg attttgattg tcccgtcggg aagtttttca aagttctgcg tattcttgca
    30601 gggaggatct tccttatatc caggacaggc caaaaacttt ccgtttcgcc cccacttgat
    30661 ctccaacatg cggccgcact tttcacaagg caggttggtc gggggctcaa cgatgtcttt
    30721 ggggccggga atactttgcg ccagctccat ttccctgaca aagggggcat aaaactccct
    30781 caccgcagac acccacggct tggttccctc ttcaacttcg tcgagttgct cttccatatg
    30841 cgaggtgaat tctgtgtcca gcagatccgg aaaccccttc agcaaaaagt cattgaccgt
    30901 ccgacccgtt tcggtcggca ggaaacggcc ctcgaccttc tcgacatatt tccgatcctg
    30961 aatcgtagaa atgatggcgg cataggtcga tgggcgaccg atgccctttt cttccagttc
    31021 acggatcaac agcgcttcgt tgtaacgagg cggcggctgg gtgaaatgct gctttgagat
    31081 aaggcccggc agggtctgtg cttcctgagt gaccagatgc agcgactctc cttcacggag
    31141 tacgggaagc tgccggtccg actcgtcctc cgactcttgc tcacttttag gcttctgcga
    31201 gggtaattcc ttgtcggtcc cctccaggta gacggctgtg tggccgggga atttcacgac
    31261 ggtgccggtc gtgcgaaaca cgtagcgttc tggtgttccg atcagactgg aatcgacccg
    31321 cgtcacatcc aagatagccg ggaccatctg tgaggcaatg aaccgattcc agatcagctt
    31381 gtacagattg tattgatcct gctctagaaa ttgcctgatg gattcaggat cgcgagccgc
    31441 catcgtcgga cgaatggctt cgtgcgcttc ctgggccgcc ttttgggtct tgtacacatt
    31501 aggagtcgcc ggcagatact cctctccgaa acgctcgcga atcaccagcc tcgcatcttc
    31561 ggtcgcctct tgggagattc tcggcgaatc ggttctcata tacgtaatga gacccatcgg
    31621 gccttctgcg ccgatttcca cgccctcata caattgctgc gccagcgtca tggtcttctt
    31681 cggggtgaaa tgcaatttcc tggcggcttc ctgctgcaat cggctggtga tgaagggcgc
    31741 gaccgggttt cgcttttttt ctttccgctc aatggattga acgacaaatg ctttgccttg
    31801 cacggcattg aggatttgct cggcctgcgc actcgtaccg atatcggcat cctgcccgtt
    31861 gatggaatgc agcttggcct cgaaagccgg aggattgtct ccacgcaaga gcgcgacaat
    31921 ggaccaatat tcctcggctc tgaacttttc gcgttcctgt tcccggtcgc aaataagccg
    31981 caccgcgacg gattgaacgc gtcccatgct gagaccgcgc cgcactttct tccataacaa
    32041 ctgactgccc tggtacccta cgatacgatc aagcacccgg cgcgcctgct gcgcctgaac
    32101 cagcttcata tcaatttcac cgggggactt cagcgcgcgt ttgatcgccg attctgtaat
    32161 ttcgttaaag agcacacggt agaccttgcc gtctttcttc ttgcccttgc cgtgcagttc
    32221 ctgcgcgata tgccaggcaa tcgcctcccc ttcacgatca gggtccggcg ctaagaagac
    32281 cgtatccact tgctgagctt ttttcttgat gtctgcaagg actttggatt tgcccttgat
    32341 cgtaacgtac tgaggttcaa agtcatgctc cagatcgacg cccagtttac tggtcggcaa
    32401 atctttgaca tgccccacgg aggccatgac ggaatatcct cgtccgagat acttggtgat
    32461 ggttcgcgcc ttggtgggcg actcaacgat gatcagagat ttggccatgt ggctccatgt
    32521 tcctaagtac ctgcgcccag actatcacac tcactataac aaatatcgag accctgtcag
    32581 cttgaatctc tcaacgggtc cactcagagg cgcacatact cgttgccggg caactggcgg
    32641 acacaatttt tcaattccaa ggacagcaac actgcggtga cttgcgcggc cgcaagcccg
    32701 ctgcgtcgaa tgacatcatc gaccgattgc ggcaataccg atacggcatc atataccatc
    32761 gcttcctcgg gccctaattg catagccggt cgggctcctc tcgcgcgatc gccgagccgt
    32821 tcgcgaaacg cggcatccag ctgaggcaac aattcatcca gaatatcctg cgcggattcg
    32881 acgagcctcg cgccatcctt gagcaggcta ttcggtccgc gactattctc ggccttcaca
    32941 aagccaggca ccgcaaacac ctcgcgcccc tgttcaccgg ccaatcgcgc ggtaatgaga
    33001 gagccgcttt caaccgcggc ctcggtcacc acgacgccta acgacagccc gctgatgatg
    33061 cggttccggc gtgggaagtg gtagctgtgc ggcgcggacc cgagcggcag ttcggagagg
    33121 accgccccgt gccgctcgat ttcctggcgg agcgcggcat gatcggccgg gtacgtgcga
    33181 tccaatccac atcccatcac ggcaagcgtc cgtcccttgc cggcgagtgc tccgcgatgt
    33241 gcggcagcat caacgccgcg ggccaaccca ctgacaatcg tgaagcccat ctccgccaac
    33301 tcacaggcca attcctcggc tagcacccgg ccggcggaac tgactttcct ggtcccgact
    33361 atcgccacgg cttgccgatc actttccagc agggttccct ggacatacag cagcggcgga
    33421 gggtccggaa ttgtcttgag aggcgcagga tactgcggat cgagatacgt cagcacggtg
    33481 atctgacgat gctgcaccgc cgccagttcc cgatcaagct gtttgaccgc ctgtgcgtca
    33541 ggcccacggc gaatggcgtc gatcaacgga actctgcacc cgacgtcttc caggctcgct
    33601 tgcgttgccg acagcaccgc gtccggagag cccatcgcct gcaccagctt gagcagaatg
    33661 gcatcgccca caccagggat cgcacggagc atcagccatg ggcggagaga tagactattc
    33721 accatcgtct gagttcatca ggcatggtcg gtgcgactca gtgaccgtgg tgctggaagc
    33781 gctgtcagag acgagacgtt cgcgtaaaac cagccagggc ggcgccttgc tgtattacat
    33841 gacgactaag tcgaactgtt cgagatgtag acgatcatcc ggagtcacaa gaatcttggc
    33901 gctataccgc cgctcgagtt cctctacccc gggctgttcc tgctcctgta acagcagggc
    33961 gacagcagga tgcgccccga cgacgacacg ttgctgctcg atctcgtgac cgagccgccg
    34021 cacttccctg aaaatttcat aggccaccga catgggagat ttcgtatacc cgcgcccgtc
    34081 gcagtagtgg cacggttcag agagtgaccg cagcagatcc tcgcgcaccc gctcacggga
    34141 aatttcgatc aatccaagat cagacacgcg ggaaattctg gttcgggcct tgtccgaaga
    34201 catggcatcc accaaggcgt gatagacctt gtcgcggttc ttttcccgct ccatatcgat
    34261 gaaatcgaca atgatgatcc cgccaatgcc ccgcaacttc acttggtagg caatctcccg
    34321 tgccgcttcc aagttgttcc ggagaatggt ttcttcctga tcccgtttgc cgacaaaccg
    34381 gccggtattc acatcgatca ccgtcatggc ttcggtgtga tcaatcacca gatacccccc
    34441 ggacttgagc cacacttttc gactcatcgc gcgggcgatt tcctgctcca cccccaggta
    34501 atcgaacagg ctttcttcct tgtcatagaa gtggatgcgg gacgtctgct ccggagaaaa
    34561 ccgttgcacg aaccccttga tcgcgttgta ttcctcgcgt gaatcaatca ataggcgatc
    34621 gactcgacgg ccgaagagat cccggaccac gcgaaagctc aggctcaaat cgctatgcaa
    34681 gaggctgggg gcgggctgct ggtcacgctt ggtaagcacg tcctgccaga ggacatgcag
    34741 gaactcgacg tcggagcgca attcttcttc cttcacccct tcactgaccg tccgcacgat
    34801 gtagccgaac cccggcctcc gcgcccgttt cataatttcc ttcaaacgcg cgcgttcttc
    34861 atcgcgagga atccggcggg atacaccgat atgctccacg gtcggcatga acaccagata
    34921 tcgtccgggc aaagacacat aggtcgtgac ccgtgatccc ttcgtgccga tcgggccttt
    34981 ggaaatctgc accatgagtt cctgtccctc agacagcaac tcttcgatgg gctttgagct
    35041 ttgccgctta ggcttgggaa cttcggaatc cttattgtct tcgtcgtcgc cttcgaccag
    35101 ggtatctccg ggctctacat cgatagacag atcggagaca tgcataaacg ccgccttctc
    35161 cagcccgata tcgatgaaag ccgcctgcat ccccggcaag actttcgcca cccgtccctt
    35221 ataaatattg ccgacgtaat cctgatcctt ggcccggtcg ccatagagat ccgtcacgac
    35281 accattatcc aggacggcaa cgcgggtttc ttcccgcgcg atactgattg cgatctccac
    35341 acccatacac actcctttaa ccccagatgg atcggattgg gagacgcacg cggacagatg
    35401 tccgggttct ctcgtctcgc cggcagcccg acgcccctct ggttcacatg gttatttcac
    35461 aacaagtcgg ccgctgtcca ggcatcgcct agtaaaacag ggtcagccga cgtcgcttca
    35521 cattcaataa caaacccaat gacgccatcg tcatgatggt cgcgctgccc ccataactca
    35581 ccagaggaag cggaatcccg acgatgggaa acatccctgc ggtcatgccg atattgacga
    35641 ccacgcaaaa acacaacatg gcgacgatac cggccgcgag taacgccccg agcgtgtcct
    35701 tcgcacgcgc ggcaatttcc aatgacaccc atatcaatgc gataaacaga gccagcagga
    35761 cgaggacgcc gacaaagccc cattcctccg catagacggc gaatacaaag tccgtatgcc
    35821 cttcgggcag gaacttcagc tgactttgtg tgccgccgta caatcctttg cccgacagtt
    35881 cccctgaccc aatggcaatt ctcgattgca acgcatggta gcctttgcct cccggatcgt
    35941 aatccggatc cacgaaggcc atgacccgtt cgcgttgata gtcgtgcagc gaggcccaga
    36001 ccacttccca gacgaacggg aacagcataa cggagagcaa caaaatgatg cccaacgcct
    36061 tcgaccggac gccgaccatc aataacatgg ccgcatagac cgccaaaaag ctcaatccgc
    36121 tacccaaatc cggctgcttc aggatcaaca acagccccgg caacactatc aagcccggca
    36181 acaccacccg atgcagccat cccacgcgcg atgcccggga gtaataattg gccagcacca
    36241 gcaccagcac cagtttcgcg aattcagacg gttgaaacgc aaatggtccg atgggaatcc
    36301 atcgctgagc cccgcggctc gatttcccca tgaacaacac gaccgcgagc atgattaaaa
    36361 taaccgcgta ggtcgggtac gccaggcgtg caatcttgtg ataatcggag agatacatca
    36421 cgagaaacgc gacggttccc agcagaatcc agaccagctg tttgagatag aaaggaaagg
    36481 cggtgtcctg agagtgcgtc acgctataga tcgacagcac cccgaccgac aggatgacgg
    36541 cgatcagccc catgaagcga agatcgaaac tatccaaccc ccgactgtcg atcacccgat
    36601 caatcatcac gtcttcggcc gcgtatcaac cggcaccgca ggcgacttgg cccgcccgct
    36661 tgccggtgcc ccctgcagta cctggggata ggctttcaca taggcttcaa taacgtcctt
    36721 cgctaagggc gcggcagccg atccaccgtg ccccatgtgc tcagcgagga cggcgacggc
    36781 aatgcgcggc gcttccacgg gagcaaaggc gacgaaccac gcatgatcac gaaacttttt
    36841 tggaatgtct ttctccggcc cggtacggag ggccgccgtc tgagcggtgc cggtttttcc
    36901 cccgatcgtg accaatggag actttgctcg cgttgccgtt cctttggtca ccacgtcggc
    36961 caaggccgac ttgatgagcg cgagggtggc cggtttaact gccacttttc cgcgcaccgt
    37021 gggcggaaac tctgcccgct ctcccgtcgt gcggttcatc acggcctgta ccaggcgcgg
    37081 cttgatcgac acaccgtcgt tcgcgaccgt gccgatcaca ctcgccatct gcaagggcgt
    37141 caccgtgacg tatccctgcc cgatcgcagc ggaaatggtt tcgcccggat accagggctg
    37201 attacgcgcc ttctgtttcc acgcggtaga aggcacgatg cccacacgtt ccgacggcaa
    37261 ctcgactcct gtctcctgtc cgagtccgaa ttgcttcgca tattcagcaa tcgtgtcgat
    37321 gcccatccgc tgcccaacgg tatagaaata cacatcgcag gagtggatca acgcttcatt
    37381 catgtcgact gatccatgcc cgccctgttt ccagtcatga aacaatcgat tgccgaactg
    37441 atagccgcca ttgcaacgca ccatcgtcga aggcgtgacc gtgttcgatt cgagggcggc
    37501 cgcggccatg acgaccttga atgtcgatcc gggagggtac tgtccctgcg tcgcacgatt
    37561 gttcagcggc cgccgttcgt tctggacaat ttcgacccat tgcttgttcg ttaattcttt
    37621 ggacaatacg ttcgggtcga acccagggcg actggccatc gccagaatat cgccattcgt
    37681 cggatcgaga gccacgatgg cgcctgattc atcacccaac aggtcttcag ccaccttctg
    37741 cagcttggcg tcgatggtca gatagaggtc gtcacccgcg agcggcttgt ctgacacaat
    37801 ggcacgcttc tcatggccga gcgcgtccac ttcgacactc ttttgccccg cgcggccacg
    37861 aacctgccgg tcaaaccact tctccacgcc atactgcccg acgatgctac cctggtgcag
    37921 atctgcgaaa tccggctttt caagttgatc ggccgagact tccccgacat accccaacaa
    37981 gtgcgccgcc acagcgcccg cgggatagtt ccgctgcgat tccacctgaa tcattacccc
    38041 gggcaagtcc aagcggtgag actcgatcag ggtcgcctcg cgcaatgtca gccggtcttt
    38101 gaccttgcgt ggctgcaatt ttccgccctt gccggtcgac agttttttct gaatcacttc
    38161 cgggtcgagg gccagaagcg aggccaattg cccaactaac gcctgacgat ccttcacgtc
    38221 ctccagcgtc acgtacaagg caaaactcgg cacattgttc gccagcaaca cgccgttccg
    38281 gtcgaaaatc agtccgcgcg ccggctcgag caccacggaa cgcgttcggt tattctcaga
    38341 caaatcccga taataggggc cttcccgaat ctggagatgc cagaggcgca aggcgagcaa
    38401 ggccacaacc aacagcaagc ccacacgcag aatgaccagt cgccgctgaa gatcgagaag
    38461 atcggagtcg ttgaatccta ctgtcgccat cgttgccctg cgccggtctc ttgccagaga
    38521 ccgttcctca tttagagggc tccctccgtg accatacgat catcagacca acgttgacgg
    38581 aacaacagat acaggcccgc gccgagagcg gcgtcaagtc cggcctgtac cagcacggtg
    38641 gaccgcacgc ccaaccagac ttcatccagc cctgcgggat tcatcgaata gagaaagacc
    38701 gcgctcgaca ggcaggagat gatggcgagg ccggccgtca tgacagccgg agtgatgtgg
    38761 gccatatgcc tcccggccag gccggccagg aacccgcttc cgcccttggt caccacattg
    38821 atccacaaat ctcctgctga cagcatatct tgaaaacatc ccagaatgag gccgagaatt
    38881 aaaccgtcca gctctccgcc gagaaatccc accaggcagg ccgctatcag cccgagatcg
    38941 ggccgcacac cgaatacgct ggcgtaatgc agcaatgtcg tttgcaaagg aaccaccacc
    39001 agcgccagca ggaggtacaa caggaacttc atggcttttt tctgtctcca tgaatggcct
    39061 gaagcaattt ctgcgcggcg tcggcatcct catagggagc ggtgatgatc aacacctcat
    39121 ccagtttgga gaggtccact tccggctcga tttccgcgga ttggaacaaa tccccttctg
    39181 acttttcgac ctgaatcagg cttccgatgg ccagcccccg ggggaacgcg ccggtcaggc
    39241 ccgacgtcac cacgcgatca ccggcctgaa cgttcgataa cagcgggatg tacttgagtc
    39301 gcgcccgccc atgactggtt ccctcgacga taccttcatc acgggttcgc tgcacgacgc
    39361 cggcaatcgc attgttcggg tccgtgacca agagcaccac cgacgaggtc gcattggttt
    39421 tcaccacccg accgacaaca ccggcagggg tgatcacccc catttccgtt ctcactccgt
    39481 cattctcgcc tttattgagg atcattccgc ggtaccagtt cgtggcatcc cgcccgatca
    39541 cctgcgccgc cagggtctgc gatggcgatt gctgcttaaa attcagcaag gcctcatacc
    39601 gttgcacggc tgtcatggac tcgcgcagct gattattctg tcctttgagt aactcgatgt
    39661 cgcgatgaag ctggcgattt tcctcacgca ccgtctgcaa ggcgacataa ccgttccaca
    39721 tgtcggcaat gccatgatcc acggaggaaa atgcggcgag tggaagactg aggacttgac
    39781 cgagcggtcc accgacgtac tgcagcaacc tctggctttg actagggaga agaaagaggg
    39841 cgacgagcag gcaggcgaac aagacgatag caagacgccg ggtgccgtat gttgagcgcg
    39901 atatagccat ccacactcgg gatggaggtc atcgagaggt actgcactgc gacataaccg
    39961 aaaccttgcg aagaaggtcc aactcatcga gaatcttccc gacgcccaag accacggagg
    40021 tcagcgggtc atcgaccgta atgatcggaa gattggtttc ctcacgaaac cgagtgtcca
    40081 ttcccttcaa caaggatccg ccgccagtca gtacgatacc gcgatcgatg atgtctccgg
    40141 ccaattcagg cggtgtgttt tcaagcgcca ccttgatggc attaacgata gttccaatgg
    40201 gttcctgcaa cgcttctctg acttcggcat catccaccac caacgtccgt ggaatgccgg
    40261 agatcaagtc gcgacccttg atcatcatgg tcttccgctc ttcgaaagga taggccgacc
    40321 cgatttcgaa cttgatgcgt tcggccatgt gctcgccgat gagcagatta tatttcttct
    40381 taatgtagtt catgatggca tcgtccattc tgtcgccggc cactttgacc gactcgctat
    40441 agacgatgcc gcccaatgag atcactgcga tgtcggtcgt gccgccgccg atgtcgacca
    40501 ccatgtttcc cgacggttcg gtaatgggca aaccggcccc gatggcggct gccaccggtt
    40561 cttcgatcag atagacttca cgcgcaccgg cgagttcggc agaatcacgg acggcccgct
    40621 gctccacctg cgtaatccgc gagggtacgc cgatgatgat gcgaggccgc acgaatgccg
    40681 tgcgattgtg agccttctga atgaagtggc tcagcatcgc ttcggctttt tcgaaatccg
    40741 caatgacgcc ttccttcatc ggacgcaccg cgatgatatt tcccggcgtg cgtcccaaca
    40801 tgcgcttcgc gtcggcgccg acggccatga cacgttccgt ctttttctca acggcgacca
    40861 cggaaggttc gttgaggaca atgcccttcc cctggacgta cacgagagtg gtcgccgtcc
    40921 ccaaatcgat cgccagatca ttggagaacc atccgaacat atcactcatg aaggccacta
    40981 gaactctccc ttcacacgat tgcttcgttc agcagcatcg agccattcag tccgatacat
    41041 ccagctgtcc gctctccagc acctgcgtct tggcaatttc gtcgcccaac cggcggcctt
    41101 gctcattgcc gatgaccaat aatccttcta gcgagagtac cgcgccccat ccgatccacc
    41161 ccacataggg tatctcaaat gccaactgag caagaccaca gggcagattg cgaatgatcg
    41221 actcccggaa tcccgccggg tcccgcgtac gtggaacgat ggtttggagt ccgatcagcc
    41281 gctttccgac actgcgacca ccaccaaacc cgtcgcccaa caaaatatag gcaagtccgg
    41341 ccaggacacc gatgggaggg accagtttgc tcaccgccgc cacgatcagg agatcgatca
    41401 ttttcgcgat gaagcgattc agcacctggg ccttgggata gacgcccagt tcgaccgggc
    41461 tgcgcccgat tgcctcctcc gccaccggaa atctccccaa ttcttcgaca ctatagcaaa
    41521 tctctttgac ctgcacaaac aaagtcgcaa tcttctcgca cccgctctcc gcgcacttct
    41581 tcctcttgcc tttgctgtcg tcgcctgagt agaatccgcg aaaccctgcc gagaagaggc
    41641 gcattcgatg atcaaattat tacgcgaagc tgcacacgat tacccgtggt ttctcaaatc
    41701 catcatgggc acgctggccc tggcgttcgt cattacgatg ggctggtggg gattcggtga
    41761 ccagagcggc aatgtcgtgg cgtcggtcgg ggaccaggtc gtgccgctgg atgaatatcg
    41821 ccgtgcctat gaaaatacgt atcgcttcta caaagacaaa ggccagaacg acatcaagga
    41881 tgaattcctc aagcagtttg tgctggaaca actgatcgat aatcgcatgt ggctgcacgt
    41941 ggccaaagaa atgggactct cggtctccga tgaagatttg cggaaagcca tcatgcagcg
    42001 aacggagttt cagaagaatg ggaacttcga tcccgagtcc tatcggcgac tgctggcagc
    42061 aaatcgcttg acccccgcct cgttcgaagc catggaagcc aaggatattc tcaccaacaa
    42121 ggcccgcctg gtcatcatgg atgccgtcgc gttaacgcct tctgaatatg cagaggcaca
    42181 gacgctcgtc tcacgcgagg gagaatctga tccgacgaaa gcggcgattg cgaaggaacg
    42241 gattttccag aatctgctgt tccagaagca gcagcgggcg ttgatggctt attcggaatc
    42301 gatgaagagc aaagttcccg tgaagatcca caaagagctt atgtagcggc ggacagtcct
    42361 cccgcgatca acatcgcatc tccgtaactg tagaacctgt accgttccga caccgcctcc
    42421 tcataggcag cccgaagcgg ctccagtccc acaaacgcgg acaccagcat cagcagcgtt
    42481 gttcgcggca ggtggaaatt ggtcaccaac gcgtcgatca cctgaaattc aaaccccggc
    42541 gtgatgaata attcggccgg accctggaaa ggctggatgc cacgacctcc tcggcctgcc
    42601 gtttccaatg cgcgcacgac tgtggtcccg accgccacca ctcgatttcc tcgcttctgc
    42661 gccaggcgaa tctgctcgac cgtacgagca gtcacctcca cctgctcggc cagcatccgg
    42721 tgctcttcaa tccggtcgct cttcacgctg cgaaacgtgc cgggccccac gtgcaacgtc
    42781 acctccgcca actccatgcc cttgccggtc agggtctcca ccaattcggg cgtaaaatga
    42841 agtcccgcag tcggggcggc aatggcgccc tcctgttgcg caaacatggt ctgataccag
    42901 cgctgatcgt cctgcgaggg ctcgcgtttg atatagggcg ggagcggcat cgtcccggta
    42961 ttgtgcatga gatcatacac actcgtcaca ccagtcacct tgacggtcgt cctggcctga
    43021 ctccgctcca cgatttcgcc gcatccgccg ccgggaaaat caatgaacgc tcccggcttg
    43081 aaccgtcctt tgatcaatac ctcccacagg ccctgtccca gatcctgcac gaacagcaga
    43141 tcgagaattt tccccgtcga gcgcgtctgg gccggaaccc gcgccggcat gaccttggta
    43201 ttattcacca caatcaggtc ccccggctgg agcagcgatg gaagatccgc cacccggcgg
    43261 tgcatccgtg cgtggctgct gcggtccatg accagtaggc gcgcatcatg gcggggtagt
    43321 accggacggt cggccacaag tgccggatca aatgggaaat caaactcgct gagtaacatc
    43381 agggggttgg aataaccggc atcatcaact gcgataaggc cgagtttcgt aattcagttc
    43441 caggatagta atactgcaga atactgttgt acgaatatcc gagttcagcc aattcttttg
    43501 cgccccactg acacagcccc accgcatggc ctgcgccgta tccggcgaac accacttcgg
    43561 caccgatcga ttccacctcg aattgcgtac tggggatgac agtatacccc gcggccttcc
    43621 gcagatcttc gccccgcaac agcgtctcgc cgccggaatg caaaatccgc aaacgcgcga
    43681 ctcttcccgc acgactgaag gcaatcggcg taatcgtggc aatggttccg accgcaaatc
    43741 cctggtgccg gagattcttc tcaagctgtt cgattttcac actggccttc cactgataat
    43801 acggcgactc cagatcaaag ggacattcga cccccttgag gtacgggaga tctttattgg
    43861 cccacacatt caccgcatct tcagtcgggc ccgccgcggt tgaggaaaag gcggcgtaaa
    43921 tcggcgcctg ttgatgggtg acgacgatcc ctcgggttga ttcgacggcc tcttcgactc
    43981 gatgatcgac cccggtacgt ccccgataga cctgatcctg aatcgtcgcc atgacatcat
    44041 agtcgcgtgc cgcgctgagc atcttgttgt ataacgcgta ggttcgcgcc gccacggcct
    44101 gcaccttcag catttcgggg tgccaggccg aactgacttc ggatggcacc acgcccttca
    44161 catattcctc caaatccacc tgattgacga ccgcgaccgc atgccctttg cgtgacactc
    44221 gcacggtccc actgatcgga agcggagtgc ctgcagatcc tccttctttc ccgacgacca
    44281 gggtcagatt gtgccgcagg gagcggatga gcacctgtcc gccgagaata cgccggccat
    44341 caacatgtaa cccgtccgct cgcgccgtga gatggatcgg gccatgcagg gttttcgtgt
    44401 cgcccgtctc catcacaaga gccagcggcc catccgaccg cacgtctacg aacggtgctt
    44461 cctgtacgag taagacacga atggattcgg ctgaggcaga ctgcgcgaag acgatcccag
    44521 cgagcgccat gactcccgct gcgaggaaac tccgggcacg atgtgggatc agcggcgaaa
    44581 gatccatgaa aggatataca acacgacgct caacagaatg ctgagcacaa gactggttgt
    44641 aatcggaaca tagaggctga agcgttctcg cgtaattgac agatcgccgg gcaaccggcc
    44701 gatccatccg aacgccgcgc cggcaccgga ccatcgttca agcaacgtca ggagaattcc
    44761 aacgccggcc agagccaacc cggcaacgat gaacagccgg ccgatcccgc tccaagcacc
    44821 catagatcat tgtatcaaac attcggcaat ttgaacggcg ttcagcgcgg cgcccttccg
    44881 gagattgtcg ctgacgaccc acagattcaa tccattggtg atggattcat ccacgcgcac
    44941 ccgtccgaca tacacctcgt ctttgccgga gacatcgaac ggcatcggat agagtttttt
    45001 caccggatcg tcatagacaa tcacccctgg catttccgcg atggccgccc gcgcatcatt
    45061 ggctttcatc ggacgctcga gttccacgtt aatggcttcg gaatggcagc gcagcaccgg
    45121 gacccgcaca gtcgtcgccg tgacccggag cgtaggcatg tccaaaatct tgcgcgtctc
    45181 ctggacgatc ttgacctctt ccgaacaatc cccaccgtct ccgaacgacc cgacttgcgg
    45241 caagaggttg aaggcaattt gatagggata gacttgcact ttcacatcct gaaaggagat
    45301 cagcgccttg gtctggtcca ccaattcatc catggcggcc gaaccagttc cggacaccga
    45361 ctgaaaggtg gtgacgacga cgcgtttgat ccccgcaaca tcgcgcaggg gcttgagcgc
    45421 cattactaac ggagtcgtcg tacagttggg aatcgccacg atgccgcgct tcatagactg
    45481 cagcgccgcc gcattcacct ccggcacgac aagcgggacg tccggatcca tccggaagac
    45541 ggcgctgtca tcgatgacca cgatacccgc cgcgcccaat cgcgcgccat agtcccggct
    45601 aatggcatcg gtagcggaaa tgaacgcgat gtccactccg gcaaaagagg aggcctccgt
    45661 cagttcctcc accttgtact ctttgccctg acaggtgagc acctcgccgg ccgaccgctt
    45721 cgacgaaaaa agccgcagcg tctcaatcgg aaaattgcgt tcttccagaa tctcgagact
    45781 ttctttcccg accgctccgg tcgcccccag caccgccacc gtataggcct gtttcttctt
    45841 caacatgata ctgtccttac gcagtcgccg tgagaaccct gatgcggtga ttgaacgtat
    45901 ccgccacata cacggttccg gccgcatcga ctgctacgcc gaatggataa ctgagactgc
    45961 ctgccaacgc gttgccgccg tctcccccga actgcgcgac gccggtccct gacacgcgcg
    46021 tgatggtttt cgtgcgccca ttccatactc gaatcaaatg actatcagaa tccgtgatgt
    46081 acacgttgcc gtcatagtca acggccagac cggccggacg ggaaagactt ggggacgcag
    46141 gctcctcagg gccctgatag cggtaggtgc cgccgtctcc cgccgccgta gtgatctgcc
    46201 ccgtcgccgg atcaaccgat cgtatccggc tgttgaaggt gtccgccaca tacaacagac
    46261 cgtcattgcc taccgccaca ccactcggcc ctgccaaact ggcctgcaca gccggcacct
    46321 gatcgccgct gtaggcggcc gatccatcgc cggccgccgt agtgatgacc ccggtcgcga
    46381 agtccacccg gcgaacgcga ttattgctct gatccgcaat gtacaaggca tcatcggtca
    46441 cagccagtcc ggtcggctca ttgatcgccg cctggaccgc cggcccgcca tcgccggaat
    46501 accgcgcctg acctgtgccg gcaacgtgag tgatgatgcc ggttttcgca tcgaccttcc
    46561 ggacgcgatg attcatcgtg tcggcaatgt agagattccc cgcacgatcc acagccacgg
    46621 cactcgggaa attcagcctc gcgcggcgag ccggtccgcc gtctcctgat tcctgctcaa
    46681 tcgtcagccg tcctccggtg acataccgga cggtgccgct cagatccgtg acctgtgtgt
    46741 acgccttggt gctgtccccc gaggtgtcgg caaaaggatc ctcttcttct tggggtggca
    46801 tcgcaacagg ttccggcgcc atcatggccc cacccgttcc gccaggaccc actccagcga
    46861 ccgtcgtaat caggcctgtt ttccgatcga cccggcgtac gacgtgattt tcagaatccg
    46921 cgatatacac attcccttcg cggtcgatgc aaagcccttt cggctcattc aacgaggcaa
    46981 ctgcggcagg tcccccgtca ccggcatatc ctggctcgcc gttccctgcc agggttgcaa
    47041 tcattgcgga tggtgtggcg ctgatcgtca tggcctaggc gggaaggttc ttcacgatcg
    47101 catcacccat ctcagtggtc ccgaccaacc gcatgccttc cgcatgaatg tccttggtgc
    47161 ggaatccctg ctcaagggtc ttgacgattg cctgctcaat ggccgccgct tctttgtcca
    47221 attgaaatgc ataggacagc atcatggcgc cggaagcgat ggtggcgatg ggattggcaa
    47281 tattctttcc ggcgatgtcc ggcgcgctcc catgaatcgg ctcaaagagg ctcaccttgg
    47341 cgccgacact ggctgagggc agcatgccga tcgatccggt gagcatcgca gcctcgtcgc
    47401 tcaagatatc gccaaagata ttgttgcaaa gcagtacatc gaactgacgc ggattgcgta
    47461 ccaactgcat ggcgcaattg tcgacataga tatggctcaa cgtcacatcg gcatagtcct
    47521 tctgaacctc tgtcaccacg cgtcgccata attcggacga ctccaacaca ttggccttgt
    47581 cgaccgacat gaccttcttg cgacgcttgc gggccgcttc gaacgcgacc acggcaatcc
    47641 ggcgaatttc ctcggtcgta tagacctccg tattaaaccc gcgctctcca ccgccgggca
    47701 ttttttccac gcccttcggt ttgccgaaat agatgccgcc ggtcagctcg cggatcacga
    47761 gcatatcgat tccttcaatg acctctcgcc gcaaggtgga ggcatcggcg agcatgggat
    47821 agagtttcgc cggacgcaaa ttcgcataga gtccgagctg ttcgcgaaga cccagcaacg
    47881 cccgctcggg acggatgctg taatccaagc cttcccattt ggggccaccg acggcgccga
    47941 gcaagacggc atcactgtgt ttcgcgagtg tgagcgtctc cgcaggaagg ggcactccga
    48001 ccttatcgat ggcatggcca cccacatctg ccgcttgaaa ttcaaaggta tgcccgaacc
    48061 gctccgctac ggtcgttaag accttgaccg cttcgggaac gatttcacgg ccgactccat
    48121 ctccggccag gactgcgatt tttgctttca cgtcgcgctc tcctctcaac tattcaacga
    48181 ccgcctcgtc ttccgccttc caggcgggat cgaccaggca gagaaactct atcgattccg
    48241 ttccagtatt gatcagcgac tgtttggcgc ccggcggcac atagataacg gagccagcct
    48301 gaaccggcat tgaggcttct tccaccttca tcacgccgcg cccggcgatg aagtagtaca
    48361 cctcggacga tttcaggcga tgccacaggg actgcttgcc cggatcgagc agcccatggg
    48421 cgaggctata ccccagctcc agctcggcct ttcccggatg cagcaattcc cgcaatctcg
    48481 tgtgatcgcc tgccagaaat tcagggcatt gttgaaggtg cgtgctgaac atcgtgtgcc
    48541 gattgtgacg atagggggca atacaagatg tcgccccgcc ctgaccaaca ccatgtgctg
    48601 accaggacgg aggaatctac cttgcaggtt gggatcaaat caagctgacc ttgtgaatcc
    48661 cttcggcttg cttcgccgca aaatacgcca gcttattgag cgcgttcaga tacgccctgg
    48721 cggccgcggt gatgatatcc gtatccgcac catgaccgga gacggtccgg ccatcttcct
    48781 cgagccgcac cgagacttct ccttgcgcgt ctgtcccgcc ggtaatggca ttaaccccga
    48841 acatcagcag cttgctcttg gtttgtgtca tagcggcaat cgtccggtag accgcgtcca
    48901 ccggtccatc tcccgtgccg ctgtgcgaca ccactttccc gtcaatctcc agttccaccg
    48961 tggcttttgg cgtcttattc gttccacttt cgacctgaaa cgacttcagg accacccgtt
    49021 cggacatctt tgccacttct tcggagatga tgacctcgag gtcttcctcg taaatctctt
    49081 tcttttgatc cgccagccgc ttgaagcgtt caaaggcgtg attgacctca tcttccgtga
    49141 gcttgtagcc caggtcttcc agccgctgcc tgaaggcatg gcgcccagac aacttgccca
    49201 tcactaactt gacctgctcc aacccgacgg actccggccg catgatttcg taggtggtct
    49261 tatccttcag caatccatcc tgatgaatgc ctgaagtatg agcaaaggca ttcgcgccca
    49321 cgatggcctt gttcggctga atgaccatgc cggtgatctt gctcaccagg cggctggttt
    49381 tggagatctc ctccgtcacc accttcgtgt cggcgccgta ccaatccctg cgtgtccgga
    49441 gccccatcac aatttcttcc agcgaggtgt tgccggctcg ttcgccgata ccgttgatcg
    49501 tgcactcaac ctgaccggcc cccgccacta ctgcggcgag actattggcc accgccattc
    49561 ccaaatcatt gtgacaatgc acggaaatga tcgcctggct gctgttcggc accgtgtcct
    49621 tgatgcgttt gatcaggccg ccaaactctt gtggaacggc ataccccacc gtgtcgggaa
    49681 tattaatcgt tccggcaccg gccgcgatga ccgcctcaat cacttcacag agatacgctg
    49741 ggtcagaccg gctggcgtcc atgggagaaa actccacatc atcgacgtag gtgcgtgcca
    49801 attgcaccat ctccacggct cgtttcttcg cttcttcacg cgtcatccgg aactggtgct
    49861 tcaaatggat atccgacgtg gacagaaatg tatggatgcg cactctcggt gcgcccttca
    49921 acgcttccga agcccgcgta atgtcttccg gacgagcccg cgcgaggctg cacaccaccg
    49981 gcccctcgac ttccaaggcg atccgacgca ccgcttcgaa atctccgggc gagctgtagg
    50041 cgaacccggc ctcaataata tccacgccga ggcgggccag ctgcttggcg atcataagtt
    50101 tctcttcgac gttcatgctc gcccctggcg actgctcgcc gtcccgcaac gtcgtgtcga
    50161 atattctgat catccgagtc atgacaaccc tcctctgtcc tgcaccgtgc tcaaacgggt
    50221 tttccaacgc ggccgcaggc gacgaaaaca ccggaggcgt acccgctggg gtacgtcgag
    50281 gatgttttca agccgagaac aaagttggaa acctgtttca gcacggtgcc aataaaaaaa
    50341 gcctccgccc cggacaggga cgaaggcctt gcgagcttcg tggtaccacc ctgattcagc
    50401 gccggatgaa gccagccttc accggacacc ctcattaaat ccgtgacgcg gatcattcgg
    50461 gactcattac cacctcatca gaggcttcac aagtccggct cggaggcgag ttcaggaagc
    50521 aaccggctgg ttcgcaccgt ccaccagctc tctcaattcg gctaccgctc cctactactc
    50581 ctcgtcaatg ccgttacgac tctcttgttt cgacgaccgg tccatcagtt ttcgcgaccg
    50641 gcttgcctgc actcttggcg atcatcgtcc agaggcgtgc aatcggacct gacactgcat
    50701 accccgtgaa tgtcacgaac agcatagctt gaggccacgc gacaatcaac atcaaggcca
    50761 aaatgcccca gacgagatag gtgaagtgat cacctcgccg gaacttcata tccttgaagc
    50821 tgcgatactt gatcgtgctg accatcagaa atgccaaccc gaacgtaatc accagaatca
    50881 gcagcggttt cacttccgat cccagctggg tgatgtggag atcaaagatc acgagggaag
    50941 caatgacacc cgcagccgcc gggatcgcta agccggtaaa gtatttgctg tctgacgtac
    51001 tcaccgtggc attgaatcga gcgagccgca cagcgcccat cgcgacatag gcaaacatga
    51061 ccgccacacc aaacatgccc tgcccgctca gggcgtagga gtaaatcagc accccgggcg
    51121 cgacgccgaa ggacaccaca tcagaaagag aatcgtactc gatcccgaac tgaccggtgc
    51181 tgttcgtcaa tcgcgccagc ttcccgtcca acatgtcgaa aatcataccg acgaggatcg
    51241 cgatggcggc gactaaatgc tgcccgttga atacggacag aatggcaaac acgccgcaga
    51301 ataaattgcc cgtcgtacaa aggttgggaa tcaggtacat cgcctgccgc ttccggtgcc
    51361 ccttcgcaaa cggaggtcgc atagttggtg atttcatcgg agttccccca cgatggtttc
    51421 gcccccttta acgcggtcgc ccaacgcgac gcaaatcttc gacccgagcg gaagaaaggt
    51481 atccattcgc gaaccgaacc gtatcaaccc gaaccgttca ccacgctgca cgcgatcgcc
    51541 ttgcccgacc cagcagacga tccggcgggc aatcagccct gcgacctgca cgcacaggac
    51601 tttcgcccct gactcggtct tgagcatcac ggcattctgc tcgttatgca gcgtcgcttc
    51661 cggcttgctc gccacaagga actgaccggg ctgataactc accccttcga cggttccagc
    51721 acaaggcatc cggttcacat ggacgtcaaa cacgttcaga aataccgtga tccgaatgct
    51781 tttttccttg agataccggg gttcgaattc ttcttcgatc gcaatgacct tgccatctcc
    51841 ggatgagacg accacattgg gttgctgtgg aatggtccgg ctgggattcc ggaagaacca
    51901 ggcggtaaat agcaaaaaac cagccgctac gaccgtgggt atggtccacc ctgccaaccc
    51961 gaacaacagg gtggcgcctg cgagaccacc gacaaacggc aagccttctt tgacaatcgg
    52021 aattccgacg gctcgatcag ccatacccct ccaggccctc tatcatgcta ccacgaagaa
    52081 tccgccggtt gagttagttt ttgttcttat cgacgagttg gtccttcttg agccagggca
    52141 tcatggaccg gagccggccg ccgacttctt cgattggatg cccctcgccc tttttcaaca
    52201 aggcgttata gacagggcgg tttgcctggt tctccaacac ccattctttg gcaaactgtc
    52261 cactctgaat ctcgccgaga atcttcttca tttcctgctt tgtctgctcc gtcacgacac
    52321 gcggaccacg ggtaatgtcc ccatactttg ccgtcgtgct gatcgaatag cgcatgttgg
    52381 caatgccgcc ctgataaatc agatcgacaa tcagtttcac ctcatggaga cattcgaaat
    52441 aggccatctc aggcgcatac cccgcctcca cgagcgtttc gaatccggct tggatcagag
    52501 acgtcagccc accgcacagg accgcctgct ctccaaagag atccgtctcg gtctcttctc
    52561 tgaaattcgt ctcgatgatg ccggcccgtc cgccacccac cgcgctggcg taagccaagc
    52621 cgacctgccg ggtgttcccg ctgggatctt ggtggacggc caacaagcat ggcactccgc
    52681 tgcctttcgt gtattcggac cggaccaggt ggccgggccc cttcggcgca accatgaaca
    52741 cgttgacgtc tgccggcggc acgatctgtc cgaagtggat attgaatcca tggccgaacg
    52801 ccagatagga accgggcttc agattgggcg caatatcctg gcgatagatc gccgcttgcg
    52861 cttcatcagg ggcaagaatc atgatgacgt ccgaggcttt cacagcatcg gcaaccggca
    52921 tgaccttcaa accgctagcc tccgcttttt tccatgagct tccctcgcgc aaccccacca
    52981 cgaccgtggc gccgctttct ttaagattga gcgaatgcgc atggccctgg ctcccatagc
    53041 cgatcacggc cactttcttt ccgcggatga gctgcaagtc ggcgtccttg tcgtaataaa
    53101 tcttcatacg tcactcctga atgttcatag ggcacatgga tggaaacaat cgtgcacaga
    53161 gaggatgcgc gcgttattct cgagcgagct tcttcggttg gctgagggcc gggcggatcg
    53221 cctcacgcgc gatcgctact cgccccgtcc ggatcagctc cttgatgccg agcggctgga
    53281 gcaagttgat gatcgcctca agctttttcg gatcgcccgt gacctcaatg gtataggtcg
    53341 atggtgtgga atcaatcaca ttggcgcgga aaatgtccgc aatccgaagc gcctcggccc
    53401 ggtcttccgc cttggtatgg actttaatca acgccgtctc gcgcgaaacg aactcgcttt
    53461 cattgaggtc cacgaccttg atgacgtcga tcagcttgtt gagctgcttc acgatttgct
    53521 caacgattcg ctcatcccct gaggtgacga tggtcatctg cgacatggac ggatcgaggg
    53581 tcggagcgac cgacagactt tcgatattga atccgcggcc gctgaacaat ccggccacgc
    53641 gtgataacac gccaaactta ttttcaacgg tgactgaaat gatatgttcc atcactatgc
    53701 tctatggtgc tagctgtcag gctgacagcg tgttttacgc ggttaaaatg gtgtctttgt
    53761 tttcatccga tccggtactg ggcacaccgg ccattcgctg tttcaactca ggcgggtccg
    53821 caagaatcat ctcgtgattg cagccgcccg ccgggatcat cggataacaa ttctcatagg
    53881 gataggtggg cacatccacg accactggcc tgtcggtcga gatggcctcc ttcagcaccg
    53941 catcgaggtc tcccacctta ccggcgcgta gtccgactgc gccataggct tccgccaatt
    54001 tcacaaaatc cggcgtggtg tccaagtagc tggaggcata ccgcccttcg tagaacaggt
    54061 cctgccactg gcgaaccatg ccgtggaaac gattattcag aatgatgatt ttgaccggca
    54121 gcttcgacac caccgcggtg gccatttcct gcatattcat ctgaatgctg ccgtctcctg
    54181 ccacacaaag caccaatcga tccctgaagg cagcttgggc ccccatggcg gcaggaaagc
    54241 cgaatcccat cgtgcccaat ccacccgagg tcaaccagcg attcggccgg gccagtttga
    54301 aatactgcgc cgtccacatc tgatgttgcc ccacgtccgt cgccacgatc ggatcacgat
    54361 ccttcgtcag ttcatacaac cgcttgacga catgctgcgg cttgatcgcc ccgtccggct
    54421 cttgttgata ggccagcgga tgcgcctgct gccattcacg gatttggtcc caccagggct
    54481 tgcgaagatc tttctgattg ccgttgaccg tggcgcggag aatttggttc aactcccgca
    54541 gcaccgcctt gcaatcaccc acgatcggga tatcgacatg aatgttctta cggatcgagg
    54601 ttggatcgat atcgatatgg atcaccttgg cgaacggaca aaactccgac accttcccgg
    54661 tgacccggtc atcaaagcgc gcacccacgg cgatcaccag atccgaataa tggaccgcca
    54721 tgttggcgca gtaggtcccg tgcatgccca acatgcccat cgattgggga tgctcgccgg
    54781 gaaacgcgcc gaggcccatt aaggtcatat cgacagggat ctgggtcatc tctgccaatt
    54841 caagcaactc tttcgacgcc tgcgaaaata cgactccgcc gccgacatac agaatcggct
    54901 tcttcgcctt catgatcgct tcagcggcct gcttgatctg ccatttattg ccttcgtagg
    54961 tcggattgta tccgcgaatg gagacagagc tcggataggt gaaatcggcc ttggccatgg
    55021 acacatcttt gggaatatcc accagcaccg gtcccgggcg cccggttgtc gcaatgtaga
    55081 acgcttcctt aatggtggtc gccagatcgt tcacatcctt caccaggaag ttgtacttgg
    55141 tgcagggacg actcaggccg atgttgtccg cttcctgaaa tgcatcattg ccgatcaagc
    55201 tcgtggacac ttgtccgctg atacacacca gcgggactga atccatatag gcatcggcca
    55261 atgcggtaat cacattggtc ataccgggac ccgacgttac taaacagacg ccggcctttc
    55321 cggtggcctt ggcataccct tcagccatat gcccggcacc ctgttcgtgg cgggtcaata
    55381 cgacttcaat atctttctgc tggtgcagca tgtcgaaaat cttcaacacg acgccgcccg
    55441 gcaaggcgaa aatcgttttc acgccttcag cttttaagca ttcgatcagg atttcggacc
    55501 ctgtgagctt catatcaccc tccccttcgc gcatcattag cgaaggacgt gtgccgtcac
    55561 tggaaacagc agcaacctgc gtcacgtttg tgtcacagac tgctacgatc tttctgaaaa
    55621 atcaatgggt aagatggcgg atcgtatcac cagctcgcaa gtgaggtcaa gagagtcacg
    55681 acaggaactc gttgtgcgac agactagtgt cgatcaccgc cccgtggtat aggaacgttc
    55741 tcgtgttgtg agcctagtgc caatgagtcc catgcgcctc acgatcatgt cccgtcgcga
    55801 atgtcacttg tgccatgtgg tggcccgagt ggcagcccag gtgcaggagg atctcgcgtt
    55861 ccatctgacc atcgttgata tcgataccga tgaacagctc cggcggcaat acggagaccg
    55921 catccccgtg gtgctattgg atgaccgcga acttctgtcc ggcaaggtca ctgcgggaga
    55981 tctacggaag gcgataaaaa aagcgcgttg gagagaccct ataagccgga ttctgtcccg
    56041 tataaagctt gcgcttacac gggggtgatc atttctctgg gactcgagtt gccccgagcc
    56101 tcaagcaacc tacccggaga cttcggccgg gccagccttt ccgctgagct cttgcgagcc
    56161 cgcgggcgtc cccctatttg gtcttgctcc ggacgacgct taccgtgccg ttgatgtcgc
    56221 catcaccgcg gtgggctctt accccgccgt ttcaccctta cctgagccgc cgggagtgac
    56281 cgaagtccct cacgcggcca tcggcggttt gttttctgtg gtgctggtgt cggatcgctc
    56341 cgcctggacg ttatccagcg tccttgccct tggagtccgg actttcctcc cgccgagcag
    56401 gctcggcgag cgagcacccg tgtcccccca gcgcgcggag gaaagtatac gagtcccccc
    56461 aggccgattc aagtaagaag ccgggcggac ttaatgcaat gcgggcaact cgtggttggg
    56521 tgcaggcggc ttcgactgat agatgggcat ggcctccgcc atccactcct ccagctgttg
    56581 aattcgggtt tcgtggctcg ggtgggtgga cataaattcg gacggagatt tacctccgga
    56641 catctcgccc attctttgcc acaactggat cgcctcacgc gggtcatagc cggcatccgc
    56701 cgccagcaac acaccgacat agtctgcctc tgattcgtgc ttgcgactga agggcagcaa
    56761 caccccgacc tgcgcaccga cacccaatgc cgccatgcct gcctgcgaga gcaccggatt
    56821 ggcgccactc acccccagcg cgatgccgat cgcttgtaag gtggtttgtg ccaatgtgtt
    56881 ctgactcatc cgttctccgc cgtgacgagc caacgcatgg acgacttcat gccccatcac
    56941 cgccgcaagt ccggcctcgg tcttggctac ggggaaaatt ccggtataca ccgcgatttt
    57001 cccgcccggc agggcaaagg cgttcatggt cttatcgtcc ttgatgaccg tcacttccca
    57061 ttcaaattca ttggccacgt cgctgtattt ggatcgttta gcggcctcga tcactctcgc
    57121 ggcgacacgt ttgaccggct cgatctcccg agggtcggtg gagggcctca ttttgggatc
    57181 gctcttgacg tcggcatacg cctgcgcgcc catctgcact tcctgggaca tcggcatcat
    57241 catcagctgc cagcgtccgg tgtagggatt ggtttggcaa ccagtcgtca ccaacatggt
    57301 cacgatgccg aaacacagcc acagcaggtt cgatggccgg gcagatttcg accacccctc
    57361 acggttcgat ttcatcttca tcacaggcct ccgcaaattg accagtctga tatgctctgc
    57421 tagattaccg cgccgcccca cccgtttcta ctgggagttt caccgagtgc ctatcaccac
    57481 gacactcttc tcgctgaatc gaattggcat cgacgaatcc ggcaaaggcg attattttgg
    57541 acctctggtg attgccgccg tgttcgtcac gcctgcatca gaacaggatc tcgccctcat
    57601 gcaggttcgt gacagcaaga agatttccga tgggcgtatt ctcgaaatgg cccccgacat
    57661 ccggacagtg tgcccccaca gcatcgtggc gatcggcccg cagcgctaca atgagctcta
    57721 tgccaagatc aacaacctca atcgcctctt ggcctggggc catgcacggg cgctggaaaa
    57781 cctgcttcag caagtcgact gcgatctggc gattgccgac cagttcggcg acgaacgatt
    57841 gatactcaac gcgcttcagg agaaaggcaa gcagatccgg ttggtgcaac gcacgaaagc
    57901 agaaacggac ttggccgtag cggccgcatc aatacttgcg cgcgccgaat ttttattgcg
    57961 cctccaacgg ctctcgcagg aattacacac gaccctaccc aagggtgcat cccctgccgt
    58021 ggaattggcc ggacgtatgg tcgtgaaaaa gtacgggcgg gacagactga gtaccgtcgc
    58081 gaaattgcac tttaaaacaa cacagcaggt gctgaacgac acctagacgg attcgccgag
    58141 ctcaaaatcc ttcgcctggt cttggacgag agggcgcttt ccctctggaa caggctcacc
    58201 ttcccaatac aacattcgat aacaataggg gcaagtatag acgtcgtccc cgcgtttgac
    58261 ctgagaaatc aattgaggcg gcacctggag ccggcacccg atacaaatac catccttgat
    58321 cagcgccagc gcctggtctt ttcgcgtggc tttcagcttg ttataccgag cgagcaggcc
    58381 cttctcgacc cgtcctgaca attccttttg ttgagcttcc aactcggcga gttccgcagc
    58441 gagcacgcgg tccttctcgt cgagcaccgc tttctctttg ctgaaaaggg cttctgagtc
    58501 tttcagttgc acctgggcgg aagcaatcat ccgttgagtc tgttcaatct gctccatcgc
    58561 gagcaggatc ttctcttcga tctctccgcg tttcttattg gccagctcga cttcgaacag
    58621 atgggcctga tattcctggt tcgtcttcaa ctgggcggcg cgatctttca tcttcccgat
    58681 gcggtcctcg tgcgcctcca ggtccttctc gtgacttcgc cgttccttgt tcaaggtctc
    58741 gatcgaagcc gacgcgtctt gaaccgtccg ccttgcttcc cgcagaggag cctcattcac
    58801 ttcaagccgt tcgggtattc ttcgccgttg ttctttgagg tcggcgatgc ggagatcgag
    58861 tttctgcaat tcgatgagag gggaaagctg tgggttcaac gggctccttg tgagttgcgc
    58921 gagagcaacg tcggcacgac gcctctcgca ccggaatgat ggtgaggcgc tcaggctggt
    58981 gggcccacta ggacttgaac ctaggaccag ctgattatga gtcagccgct ctaaccacct
    59041 gagctatggg cccagccctg ccgccagtga cctggccgca gcctgcatca cggcatacct
    59101 ggttcgagcg gcctgattct aaagctgcca gtcggaaaga gtcaaaccac tcctgacgac
    59161 gaagactaga gactctccac gaaactacgc agcttcttgc tacggctcgg atgccggagc
    59221 ttccgcaacg ctttcgcctc gatttgccga atgcgctcgc gagtcacctc aaaatcctgt
    59281 ccgacttctt caagcgtgtg atcggtcgct tcgccgatgc caaagcgttt ccgcaacacc
    59341 ttttcttccc gaggagtgag ggtttccagt gcgccattga tctgacgttg caagtcgtac
    59401 cgaatggcgg cttccaatgg cgagaccgcc ttcttgtctt cgatgaagtc tcctaagtgg
    59461 ctgtcttcct cttcgccgat cggggtttcg agagaaattg gctcacgggc gattttcaga
    59521 atcttgcgca ccttgtcgag cggcaaatcc atgcgttcgg caatttcttc cggcgtcggt
    59581 tcgcgaccga gtttctgcac cagatggcgc gaggtcctga tcagtttatt gatcgtttcg
    59641 atcatgtgaa ccggaatacg gatggttcgc gcctggtcgg cgatcgcacg ggtgatggct
    59701 tgccgaatcc accaggttgc gtaggtactg aacttatacc cacgtttgta ttcaaactta
    59761 tcgaccgcct tcatgaggcc gatattgcct tcctgaatca gatccaggaa ctgcagccca
    59821 cggttcgtat actttttcgc gatactcacc accagacgca gattggcctc gaccaattcc
    59881 gccttcccgc gtttaacctt ttcctccgcc acatcgagat gcttgacggc atccttgatc
    59941 tcttcagcgg aaatcagcgc ttcttcctct tcaagttggc ggatccggcc cttggcggct
    60001 tgatagtgtt ttttgatgtc ctgaaggact tcttcggaga gcccggtcct gcgcttcacc
    60061 gcgagcacat ctttatgggt tcgacacaac ttccgcagca attcggcgcc ggcctcgccg
    60121 ccgacaccga gacgacgctg gcagctcgtg acttcccgct ccgattgacg gaacagcagg
    60181 ttaagatcgc gtacccgctg ggtcatgcgg tctttcagaa cgccatgcag attgaccgac
    60241 tccatcttat ccacaacctg gccacgcacc gtgtcgatct gcttgcgcag cttcttttgt
    60301 ttttcgggat cggcaccgac atgacgagcc ttgtcgtaga gatccttgag cgaggcagat
    60361 accttgcgca cggcgttcaa agaatccagc gttttcaccc ggagctcttc gtaatccttt
    60421 tcgacggctt cttcttcgaa gtcctcttct gtctccacaa ccggcacgat ctcgcgcaca
    60481 tcaatcttgc cgtttttcag ctgatcgcgg agattcagga cgaactccag ggtcatcggc
    60541 agtccgtata cgaccgtggc aatatctttc ttgccttctt cgattcgctt ggcaatctcg
    60601 atttcgccct cgcggctcag cagagccaca ctgcccattt ctttcaggta gagccggaca
    60661 gggtcgtcgg tgcggctgag cgcccccggg gtgagatcga tctccttttc gttttcttcc
    60721 gaggagtcgg attcttcgct ctcctcaatc gcttcctcgc cttcggcggc ctttggagcg
    60781 cgctccgtct cagcggactc cacaatctcg atgtccattt cgccgaacat ggtcatgatc
    60841 gtgctgaatt ggtccgacga gacgacatcc gccggcaagg tgctgttgag atcgtcgtac
    60901 gtgaggaagc ccttctcctt gccgagattg ataagcttct tcacctcgcc gagtaattct
    60961 tgtttcggca taccctactc cttcaccgca gacgtcagcg caaccggcat cacaccggct
    61021 ttccgaatcc gcatatcgtt aatcaatccg ttcaggcgat gcacttcatc ttcccgtcgt
    61081 tcgcgttccg cgaccttcaa ttcttgaatc aagaggccca tagtccgttc gcggcctcgc
    61141 cgctccaggg tctccaaaca cccggcaata tgggccggca cgtcgtcata atgctgctcc
    61201 tgcatcgaca gctccgacac caacgagccg cagacctcat ccgtgatcaa tgcatccagc
    61261 agggtcctga cggacaccct gccatcgtct gccagatgct gcaaggcaca ttcgatcaac
    61321 cgcttgtacg ccggtgccga aaacgcatcg ggggacagtt tccgaagatc cgcggcagat
    61381 aaactcccct gcacaagcaa atgcgcgaga tcgcgctctt caggattggg cttcactctg
    61441 gacgggactg agatggaggg agccttagct gtccggtccg ggcgacgctg ctcttccgag
    61501 acgagcgtgg gatagcgatc gatgagacgc tgctgactga gtcccaatcg ttccgcaacg
    61561 agccttatcc gctcttcgcg ctcgatcgga tgcgcacttt tctgaagaat ccgcaacacc
    61621 gcatccacag cccgaatccg gtcttccacg ctgcccgact ccgccgtgcg gaggctgtgc
    61681 tcaatggcaa aatccaacag gcttggcgcc gcgcgatgca aggccgcaaa cccttcggcg
    61741 ccatgttgcc ggacatacgt atccggatcg tcaccctcag gaagcgagac gaccttgacg
    61801 ctcaagccgc tattgacgaa caaatccagc gagcgcaatg ccgcccgaac ccctgcctga
    61861 tcgggatcga agagcaacac gatattcgac gcgaaccggc gaatgaccgt aatgtgctca
    61921 gcggttaagg ctgttcccaa ggtcgccgcc acatggcgaa tgcccgcctg atgcaacgca
    61981 atggcatcaa aatagccttc gacgatgatc aacgtatgct gctggcctgc ggcctcacga
    62041 gctgcgtcca acgcgaatag cgtctggcct tttttgaata acggggtttc aggggagttc
    62101 aaatacttgg gcatcccgtc accgagaata cgaccgccga atgccacaac ccgcttccgc
    62161 aaatccgtaa tgggaaacat gatccgggaa cggaaccggt cgtaatgccc tgagacggca
    62221 gttttctgtg agccgctctg atcacgtgga atagaaaggc cggctgcgac gagatccgcc
    62281 ggcgcaaatc cctctttgag catggctcta gtcagcccgt cccattccgg tggagcgtaa
    62341 ccgatgttga attgcgcggt ggtgtgctgc tgaatgcctc ggctgtcgag ataggcacgc
    62401 gcctgagcgc cggtaggccc ttgcgtcaac atacggtgat accaggcccc cgcagccgca
    62461 ttgaggcgtt ccaatcggcc gagttgcgtg cgcgcctcat tggaaactcc gccggttgat
    62521 tcaggaacat ccacccccac tttgcgcccc aaatcccgaa cgatttcagg gaaggccgct
    62581 ccggttaatc tcatcaaaaa cgtatacaca ttgccgccgg ccccgcagcc gaaacagtgg
    62641 aaaatctgcc gcgatgagct gacagtgaag gaaggagatt tctcttggtg aaagggacac
    62701 agacctttga ggttctgccc ggcccgcgtc aacgccacat gttggcccac gatttctgcg
    62761 atgtccactc ggtctctgat ttggttgatg acgtcgtccg aaatcaggcc ttggcccacg
    62821 gcggtcctcg ctagtgcccg cagggatcct accggtgaat gtaccggctg gatgcctttg
    62881 acgcgattga aagttgaccg ttcaacctaa caaacggtcc gcatatgtgt caatcaagga
    62941 gcagtgccca ttatcccgga ggccaggcca tatgccttcc gccgaggaca tgcaggtgga
    63001 gatgaaacac cgtttgtccg ctgtccgctc cggtgttcgt gacaatgcgg tagccagact
    63061 cagccaggtt cttcattgct gcaaccttgg agcaggtcat cagcaaatgc cccaataacg
    63121 cctgatcacc gtcctgacaa ttctggactg ctgccagatg acgtttcggg atgaccagga
    63181 tatggacggg agcttgggca ttgacgtcct cgaatgccaa cacacgatca tcctcgtgga
    63241 cgatttttgc cggaatggcc cggttcacga tacgacagaa gatgcagtta tccacgcgac
    63301 tccccttccg gtgcctgacg aatcccggac ttgccgaacc tggctccgag ctctcgatac
    63361 acttcgctca gcgtcacatc atggtaaccg agaaccagga gggtgtgaaa aaacagatcg
    63421 gccgtctcgt aaataatctc gtcccgtttc tggtttttcg cggcaatgat gacctcgccc
    63481 gcctcttcag ccaccttttt aaggatgcga tccacattgc cttgcagcag cttggacaca
    63541 tacgagtccg gctgcggagc ggccttccgt gccagaatcg tctgatagag ccgttcgaga
    63601 atccctccgc cggcatcctg cgtcttgcct ggttcggccc gtccatctgc actcattctc
    63661 gaaaaaaagc aggcgcgttc gcccgtatga caggtcggcc cgaccggctc cgctttgacg
    63721 agaatggtat cccggtcaca atcgacgaac agatctttca cccgcaaaaa atgtcctgac
    63781 gtctcacctt tttcccacaa tttctgacgc gagcggctcc aaaaatgaac tgatttggtc
    63841 gccagcgtct tcgtgagggc ttcctgattc atgtagccca ccatgagcac ggtgccatcg
    63901 aaccagtctt gaatcacggc ggggattaac ccttgcccgt caaacgtcat actgcggctg
    63961 tcgctcgaca ttacctgctc catttaggcg gcgcctattc gaactggaac gccacggtcc
    64021 cgcaaaaagg ccttggcctg ctgaatagta tgggtccggt agtgaaaaat cgacgcagcg
    64081 agcaccgcct cggccttccc tttgaccaac ccgtcataga gatgctccaa cgtgcctacc
    64141 cctcccgagg caatgaccgg gatagagaga ctctccgaca cagcggccgt cagtgccaga
    64201 tcatatccgt tttgccggcc atcctggtcc atgctggtca acaggatttc tccagctcca
    64261 tactctgcca tgcgctgagc ccattcgacc gcatcaagcc cggtcgcgtt tcgtccgcca
    64321 tgagtataca cctgccagcg gcctgcggcc tccgctcgct tggcatcgat ggccaccaca
    64381 atacattgcg tgccgaaacg ctgagccgcc tcacgaacaa attcaggctt ttgaaccgcg
    64441 gtcgtattga tactgacctt atccgccccg gcattcagta atgtgcggat atcatccagg
    64501 gtgcgcacgc cgcccccgac cgtcagtggc ataaacactc gggcggccgt tcgttccacc
    64561 acatcgacga tcgtctttcg attttcgtgt gaagccgtga tatccaggaa gcacagctca
    64621 tcggcgcctt cacgatcata gatggtggcg acctcgaccg gatcgcccgc atcgcgcaaa
    64681 ttgacgaagc tgacgccctt gacgactcga ccgtccttca cgtccaaaca gggaatgata
    64741 cgtttggtca acataggaat tcgcgacgcc tgaaacgtga aacctgaaac gaaacggttc
    64801 tactcctgcc cgccaaacga gacctcaacg atttccgaga gcggcgaggg cagctcgata
    64861 atccaacttg ccgtcataca aagctttgcc gataattgcg ccttccacac gagggccgag
    64921 cgcgcggacc gcttgcagat cctccacccg cgtgatgccg ccggaggcaa taacgggaaa
    64981 agccgcacag ttgacgacct cccctaacgc cgcaaggttc ggcccattca acataccgtc
    65041 gcgcgcaatg tcggtataga tcaccgcacc cagctcatgg tcggccagtt cacggaggag
    65101 atcaatcgct ttggtttcgg aaaccgcagt ccatcccttc acggcgacct tgccgtctcg
    65161 cgcatcaagg cccagcagga tacgccgggg aaattctcga caggcttgcg ccaggaaggc
    65221 ccgatccgtc agggcagccg tcccaaggac gacccgcgcg actcctgcct ggagatattt
    65281 ccgcaccgtg tcgatcgttc ggataccgcc cccaacctgc acctttacgt tgacggcctt
    65341 catcgctgac tcaatctgag gaaggttctt tggttcccca tcaacagcgc cgttgaggtc
    65401 gacgatatgg atgagatcgg ccccctgttc ctgccaacgt tttgcgactg acggcacatc
    65461 ctccgaatac acggtttcgg ccgccatatc accctgccgc agtcgcacac agcggccatc
    65521 tttcagatcg atcgccggaa ttacacgcat atccgcacct gcttcctcac ggttacttcc
    65581 cttcacccgc tgctgcgccg aacggaaacg cctccgctaa ttcggtcgcc ccataggccg
    65641 ctaactcttc cgcggtcacg aaggtcccat agcgctgaac aaacccaagc acatcttccg
    65701 tcatcggccg ctgcttttcg tagtagttgc cttcccatag gaccatgcca tcaggggcca
    65761 cgagtttcac ttcaaacccc acggcagcgg gagggtcggc acccaatcgg ctgccgacac
    65821 gttcttgata gaccaacact ttcccgctga gcgcggcatc ggccttgaga cgctgcgcaa
    65881 ttttgtgagc cggaatggtc tccacagagc cgcttcctct cgaaagatcc cgagccgcgc
    65941 ccatcgcttc ctgaggggaa atgacctgga gacccggctt gcccttcaat ttggcccaca
    66001 tcagatctgt aattttttcg cccgcaccgg gcggcaccaa atttgttcgc cgaaccgcct
    66061 gatcggtcgt gggcggaacc cccaccgcca tgtcagaacg tcggactccc gacggcacag
    66121 ggaaggatgg cccgcttgtt tcgatggcct gaggcgtcgc cattgtttca aagggaatca
    66181 gcacgatgtt gcgaacctga tactttccag cttgaggaga ggccttggag gtgaccttgg
    66241 ttccgctgca ccctgtggtc agcactacgg agagcagaat gaacagcgtt cgcgggatgc
    66301 aggtactcac gtccaggccc cgaaattctt aatcacctga agaccgacgg cctgactctt
    66361 ctcaggatgg aactggcagg cgacgacatt gtctttccac accgcggatg cgaatggcgc
    66421 tccatagtca gtcacggtgg caacgaccgc ggaatcggca ggctcaacat aataggagtg
    66481 cacgaagtaa cagtaactgc ccggtgcaat cccggcaaat attggcgcag gccgcatgat
    66541 ccggatatcg ttccagccca tatgcggcac ctttaacgaa gcatccagca caaacctctt
    66601 cacacggccg ggaatgattc ccaggccctt gtgtgttccg aattcttcac tttccgcgaa
    66661 gagtagttgt aaaccgaggc agatacccaa aaacggtttg ccggattgaa tcgcggcatg
    66721 gatcggcccg atcaactcat atcgctccag gttcgccatg caatctccga atgcccccac
    66781 gccgggcaac accacatggc tggcatccgc aatgacgcga gcctcgcgcg taaccacagc
    66841 ctgatgtccc acggcctcaa aggctttcga gacactgcgc aggttcccca taccgtaatc
    66901 aatgatggcg atcatgttgc tttcgcggac gatgcgtaat ggcagctacc actgcccccg
    66961 tctcacaccc cggatctgac tagaggcttc ccttcgtcga gagaaccgct cctgacagcc
    67021 gctcttcggg caacgtggct tggtccagcg cccgcgccaa cgccttgaag atggcttcca
    67081 tgatgtggtg aggattgcga ccatattgca ggtttatgtg cagattcagg ccgccatggg
    67141 tgacgaaggc ctggaaaaaa tcctcgaaca gccccaggtc gaacgttttg atcttccggt
    67201 ccggcaaatt cacgttatag accagatagg gccgaccgct gagatccacc gtcacctgcg
    67261 caagcgtctc gtccagtggc actgacgccc atccgaatcg cttgatcccc gccttttcgc
    67321 ctagcgcttg atgcagcgcc ttgcccatga caatgcccac atcctccacc gtatgatgct
    67381 cgtcgatgtc gatgtcacct ttggcctgca cggtgagatc gaaaaaccca tgtttggcta
    67441 gcagatccaa catgtgatca aaaaaccgaa tggacgtgtc gatcttcccc tggcctgtac
    67501 cgtccaaggt ccattcgaca cgaatatcgg tttcctttgt cgcgcgatgc aacgaggcct
    67561 gtctgggaac tccggtgttt ttcatgaaaa cctgctttga gctgatttgg catgcgcatc
    67621 gaagccttcg atatgcgcca gtctgacgat atggtccttg gctttcgtca attcttccct
    67681 ggtgtaatgg acgatgttac tgaccttcac atagtcgttg acggacaacg gcgaaaagaa
    67741 ccgggcagtg gcgcccgtcg gcagcacgtg gttcggcccg gccacgtaat cggctacggc
    67801 aggcggcgta taacgaccca gaaagagcgc cccggcatgg cgaatctgct ccagataatc
    67861 aaacggacga tcgaccgata aggtcagatg ttcgggcgcg atatcgttcg ccacctcgat
    67921 ggcctcatcc atggtaggaa cgacgaacgc gacaaaatgt cgtgcgatcg atttggccgc
    67981 gattttctcc cgctgcaagc ccttcaactg gacctgaatg agccgtacaa cctcttttgc
    68041 cagcgatgcg gaggtggtca ccaggtagac ctgcgcgtct tcgtcatgtt ccgcttcaca
    68101 caacagatcg gctgcgacat gcgccggctt cgcatcgtcg tccgccacca ccaatagctc
    68161 gctgggaccg gccaccatat cgattccgac ggtgccatag agcaatcgct tcgccgtcgc
    68221 gacatacatg ttgcccggcc caacaatctt atccactttg gcaatgctct tcgtcccaaa
    68281 ggccatcgcg ccgatggcct gcactccgcc gacccgatag atttcatcca ccccggcgat
    68341 atcagccgcg acgagcaaat aggggttgat ctcgcccttc ggcgtgggtg tgcacatgac
    68401 gatgcgtcgg actccggcca ccttcgcagg aatcgcgcac atcagcaccg aggacggata
    68461 gacggcttta cctcccggca catacacacc gacggcatca agcgaggtta ccatctggcc
    68521 gagcgtggca ttctcctcct ggtacatcca ggttttggtg cgctgccgtt cgtgaaactg
    68581 ccgcacccgc tcagcggcat accggagcga atcgccctca tctttgcgaa tgtgataata
    68641 ggcttccttg atctcctccg gtgtcacacg gatctgatca agcgtgaccg acacccgatc
    68701 gaacttcttg gtataccgca gcaccgcgcg gtctccgcct ttctccaccg cctgaaggat
    68761 cgcgcgcacg cttttctcga cagtggccgc ttgcgaacga gagcgggctg tgatttttcg
    68821 taaggctttg gcgaaggctc gatcggtact ctcgagaata gtcatgacat ccgtcctgcc
    68881 gctgcgccgc cagtatcggg cgccgcatca tgggaaccca cgactgcccg cgtgggatag
    68941 gtcggccgcg ctactccgca gccacggctg tgcgcagccg atcgatcaac acctgcaacg
    69001 gttcatgttt caatttgagg ctggcccgat tgacgatcaa tcgggcggtg gagtgggtaa
    69061 tcacctctac ctccgccaga tcatgggcct ttaacgtact gcccgtctca accagatcga
    69121 caatgcgatc ggccaatcca acaatcgggg caagctcgat cgacccatac agtttgacga
    69181 tctcaaccgg cattccgcgc tgattgaagt agcgctcggt gacgttgggg tactttgtgg
    69241 ccacccggac cttcgaggac caccgatccg gtgatggctg tccctgcaac gcggcgaccg
    69301 agattctaca cgctcccacc tttaaatcca acggctcgta gacatcgctg ccctgctcca
    69361 gcaagacatc tttccctacc acacccacat ccgcggcccc atgttcgaca taggtcggga
    69421 catcggtcgg ccgcacgatc agaaaggtgg cattgttggc ggcacagtca aataccaact
    69481 tccggctttc cacagacaac cctgggctgt aataaccggc cgccttcatg agatggagaa
    69541 ccggcccgag caatttccct ttcgacagcg cgatcgtgag tcctttcggc ttttcgtctt
    69601 gcgatttcct cgccttcacg ttcactttcc ctttctccgc tcgatgctgg cccccaagat
    69661 ccggagcttt tcttccaacc gttcatagcc acgatcgagg tgatacacac gcgacacctc
    69721 ggtggtacct tccgccgcga gaccagcgac gatgaggccc gcgctggcgc gaagatcgga
    69781 agccatcacc ggcgcgcctg tcaaacgttc cttgcccgta atcaccactc gattgccttc
    69841 gacacgaata tccgctccca tacggcgcag ctcttccaca tgcatgaacc ggctctcgaa
    69901 aatggtttcc gtaaccacgc tggttccttc ggccaccgcc atgagggcca caaactgggc
    69961 ctgcatatcg gtggggaatc cgggatgtgg actcgttcgc acattcgtcc ctttcaatcg
    70021 ccccgtgacc ttgagatgaa cgcgttcctt ttcctcaatc aactcggcgc cgctttcccg
    70081 cagtttcatc aataagggtt ccatgtgctc gggacggcac cgatcgacga tcacctcacc
    70141 accggtaatc gctccagcca ccagataggt cccggcttca atgcggtcgg gaatgacttc
    70201 atgatcggcg ccatgcaatt cggtcacgcc ctcaatcgta atcatatctg tgcctgcacc
    70261 ggcgatgcgc gcaccgcgct tgacgaggaa tgccgccaga tcgctgattt caggctcttt
    70321 cgcagcattt tccagtaccg tggtgccctc tgccaacacg gccgccatca tgagattctc
    70381 ggtcccggtg acgctcggcg tatcaaagta aatgcgactg ccccgcaagc gacgcgcgct
    70441 ggccttgata tacccatgtt cgatttcaac tgcggcgccc attttttcca aaccagccaa
    70501 gtgaaagttc accggtcgag agccgattgc gcacccgccg ggaagggaaa ccttggcctc
    70561 ccccaatcga gccacgagcg gacctaagac cagcaccgat gcccgcatgg tgcggaccaa
    70621 atcgtagggc gcttccgtcg atgcaatggc ttcggcctgc acaaccgtct gatcgccctc
    70681 ctgagagacg gcaatgccca gcatgcgcag caacttgccc atggtcaaca catccactac
    70741 tcgtggcatg ttcgacagca cacattcgcc cccacccaga atggtggaag caagaatcgg
    70801 caatgccgcg ttttttgccc cgctggtgcg gacctcgcct tggagtcgac gtccgccttg
    70861 aataagaatc tgatccatca gtctcaggga atcattggtg catcatcgat ggctatggag
    70921 gtcaggacga tgaagatgat aaggactctt cttttttctc gaaacagacg acccgttcaa
    70981 tgccgccctg atcccgcaac accacatagg tacgaaaccg accaccttct tcagcatgac
    71041 ggcagacagc tgctgcctgg ccgattccca cttccataag cagggcccca cctggcttca
    71101 aatacgccgg cgcctgtgca atcaagcggc gatgcacatc catgccgtca tgacctcctg
    71161 ccagcgccaa ccgcggctcg aaatctctta cctccggctg aagggtctcg acatccccgt
    71221 ccgggatata gggagggttg gacaggatga cttcaacctg attgggcaac ccttcctgac
    71281 cgatcggatc cagcaagtcc ccctggaggc aagtaatacg tgcctcaacc ccgtaccgtg
    71341 ccatgttgtt tcgtgcgacc actaaggcat cggtcgaggc gtcgatggct aatacgcgcg
    71401 ccgtcggcaa agccgaggcc acggatacgg ccaaacaccc tgatccggtc cccacatcta
    71461 cgatggtcgc gtctgatatc tgcacgcacc gacggactgc ttcctccacc aacaatacgc
    71521 tctcaggacg gggaatgagg accgaggggg tgacgaaaaa atcccgtccg caaaactctt
    71581 gcgtaccaag cacatactgc agcggctcac gattggctcg ccgttcgacc aacacctgca
    71641 ctccctcaca ttcttcaggc gttaaaccgc gctctgtatt gattcgctgc atgagtggcg
    71701 agagtttcaa aacatgctcc accaaccagg cggattccaa ggcgggctgg tcgatgcctg
    71761 cggcatccag gcgagattcc gactttctca gtaactcgcc gagactcgat acggaacagg
    71821 cggcgtctgc caactgctgc tgcctcattt tgtttccaga ctggccaaat gttcgtaatg
    71881 cgctctcaac gcctgcacaa tttcctccag atcgcccgcc aacacctgat caagcttatg
    71941 aacggttagt ccaacgcggt ggtcggtcac acggttttgg gggaaattgt acgtgcggat
    72001 tttctcgctc cggtcaccgg tcccaacctg cgacttacgg tcttgggcaa tagccgcctc
    72061 ctgcttttcc cgctctgcct ccacaatccg cgcccgcaag gttcgcatgg ctttggttcg
    72121 atttttcagt tgtgaccgct catcctgaca gctgacgacg acgccagtgg gaatgtgcgt
    72181 gatgcgaacg gccgagtacg tcgtattgac actctgtcca ccagcacccg aggaacaaaa
    72241 tgtatcgata cgcagatctt tgggatcaat gtggacatcc acttcgtcca cctccggcat
    72301 caccgccacc gtaaccgtag aggtgtgtat tcttccactg gcttctgtag tagggacgcg
    72361 ctgcacgcgg tgcactccgc tctcataccg cagacggccg taagctccct ttccctctag
    72421 cagagcgatc acttcacggt agccgccgat gcccgtctca gaggcatcga ccacctccag
    72481 ccgaaggcga ttccgctctg catacttgag atacatgcga aagagatctc ccgcaaataa
    72541 ggcggcctcg tctccccccg tgccagcccg gatctcgaga aacacattct tctcatcgcg
    72601 gggatctttc ggagtaagga agtccttcgc gcgagcctcg atgtccaaga gctgttgagt
    72661 caaatgctcg ctctcttctg caaagagctg cttcatgtca gccccggtgc cggaatcctg
    72721 cagcatcacc gtcacttcgt ggagttgctt ctgggcttgg gaataggcgg caaactcctg
    72781 agcaggctcc tctaactccg ttcgctcttt attgagcttg tgaatcatgc ccggctgatt
    72841 gaggaccgtc gggtccatga gctgattcgt cagctcctgg aaccgcgcag ccatcgcttc
    72901 ccattttttg atcagcccat cttccatgtc tcgcccattc cggccatagc cctgaagccc
    72961 aaaaaacaag agaccctgcc ccaaaaggaa gcagggtctc catgatgtcc tgactgaggc
    73021 ctacttgtct tttttggcgt acttcttttt aaaccgttcg acacgccctt ccgtatcgac
    73081 gatcttctgg gttccggtaa agaacggatg gcaccctgcg caaatgtcga ccttaatgtc
    73141 accaatagtg gatcgggtct tgaacgtgtt cccgcaagca cagtgcactg tcgcttcacg
    73201 gtagactgga tgaattccct tttgcatacc tgctcctcta ctgacctaga atcattgact
    73261 cgtcctgctc gccagggtgc acgactttac actggagtgg cctacactgg caagcggtgc
    73321 ctaccgattc attgattgca aaaattcctg attcgttttg gtcccaccga tcttgtccat
    73381 gaggaactcc atggcttcca tggtgcccaa cgggctcaag accttgcgca gaatccacat
    73441 cttgtttaag cggtcacggt caaccagcaa ctcttcctta cgagtgccag actgactgat
    73501 atcgatggca gggaagagcc gcttgtccgc caggcgccga tccaggtgca cttccatatt
    73561 gccggtaccc ttgaattctt cgaagatcac atcatccatg cgactaccgg tgtcgatcag
    73621 cgcagtggcc atgatggtca gactgccgcc attttcaatg ttccgagctg caccgaaaaa
    73681 ccgcttcggc cgctggagag cattagagtc aagaccgccc gagaggacct tcccgctggg
    73741 aggggcaatc gtgttgtagg ccctagccaa ccgagtgatg ctatcgagca gaatgaccac
    73801 gtcttttttg tgctcaacca gacgcttcgc tttttccaat accatctccg caacctgcgc
    73861 atgccgctga gccggctcgt caaacgtgga gctgatgact tcagctttga cctgacgctg
    73921 ccaatccgtg acttcttccg ggcgctcgtc gatcaggaga acaatgaggg taacttcctt
    73981 gtgattcttc agaatggctc gggcgatcgc ctgcagcaac atcgtttttc cggttcgcgg
    74041 agctgcgaca atcaaaccgc gctgcccctt accgatcggc gtcgtaaggt ccattacacg
    74101 cgtgcaatat tcttcccggt cgaactccag atttaaacgt tcctcgggat agagcggggt
    74161 gagattgtcg aacagaatct tgtcacgcgc gacttccggg tcctcataat taactttttc
    74221 caccttgagc aacgcgaaat accgttcgct ctctttcggt gggcgaatct gccccgatac
    74281 gatatctcca gttctcagat tgaagcggcg gatctgcgat ggggagatat aaatatcgtc
    74341 aggtcctggc aaatagttcg aatccggcgc tcgaaggaac ccaaagccgt ccggcaatgt
    74401 ctccaagacc ccctcgccaa acaccacgcc gttcttttcg gtctgcgcct gcagaatggc
    74461 aaaaatgagt tcttgcttcc gaaggtttgc cgcgccttca atctttaaat cccgggccac
    74521 gtcattcagg tcggcaatcg acttctgttt gagttccgcg agatgcataa ctt
//
LOCUS       DAOQDS010000009        72118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_114_length_72118_cov_20.026893, whole genome shotgun sequence.
ACCESSION   DAOQDS010000009 DAOQDS010000000
VERSION     DAOQDS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 72118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 72118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72118
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114_length_72118_cov_20.026893"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PK589_03380"
     CDS             complement(<1..832)
                     /locus_tag="PK589_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNJ18666.1"
                     /translation="MMRAHAVLRRREGLAAWLMVAPALLVTVVFALYPVADSLWLSLH
                     HIFVGIPQLGSPFVGLENYQALLHDPVAHQAFVVTLGFVVLSTLLELACGLIIALVIH
                     ERFRGRGLVRGAILIPWAIPTVVASQLWRYIFNDQYGFANLLLFGDRVTDYIPWLAYP
                     SVAFAVVVLADVWKTSCFAGLLILAGLQVIPDDLYDAARVDGATVWQRFRHITLPLLK
                     PALLLALLFRTMDAFRVFDLVFVMTQGGPGDATQVLQFYGYQTLFTEGRIGYGSAISV
                     A"
     gene            complement(829..2205)
                     /locus_tag="PK589_03385"
     CDS             complement(829..2205)
                     /locus_tag="PK589_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ18667.1"
                     /translation="MRWISSRQRIQRPSQSCAVTVSRWIVLLTLIVLTGSTSLGSAES
                     LPGTTATTPKPRVTLRFVSWKPDHARVWDEVLGDFTRAHPQISVVRELAPHSSTAYHD
                     LLTQKLKNRDATVDVFFIDVIWVPEFAEAGWARPLDDKFSPSMRQDFLPAAVEVGRYD
                     THYYGVPSRIDAGLLYYRQDLLEKYGFAAPTTWPELIQQAETILAGERASNPLLQSYS
                     AQFKQYEGLVCNMLEFVEGHGGSLLRPDGTGSTLSSPRALAAVQFVRDRVIGRLTSRA
                     ALTYQEPEALSLFLQGHAIFHRNWPYAWELANNRTRSTIAGQVGVAPLPGFSPGRTAA
                     ALGGWLYGISAYSPHPDEAWALIEFLSSQAVQKKFSREAGIAPSREVLFSDPEVLAHS
                     PQLRNQRSVLHAATPRPRSPVYPAISHLLQRYFSRALALEDIDLAQEAAVTDAHIDRL
                     LALTKAAP"
     gene            2525..3199
                     /locus_tag="PK589_03390"
     CDS             2525..3199
                     /locus_tag="PK589_03390"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNJ18668.1"
                     /translation="MNGLQVSAVSPRRGRLLRMTLGGLLGWALILIAITDAWAVQASP
                     VSLSFSAVQGGPNPASQVVTISKSTSRQVGWKAVDDAAWLSTAPSSGSLTSSAQVVVA
                     VNVSGLAAGTYSGSMKVTLSKGGSLSIPVTLTVASATSTKSTTTVTTATLSWAPNTDS
                     DLAGYKVYMGTTSGLYGTPVDVGNVTTYTAGNLTTGTTYYFSVTAYDQSRNESLHSGE
                     VSKSIY"
     gene            complement(3245..3631)
                     /locus_tag="PK589_03395"
     CDS             complement(3245..3631)
                     /locus_tag="PK589_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ18669.1"
                     /translation="MKVPFTLGAVWHFGLAVRDPRASAKWFMRVAGLSKQFEYEGGVG
                     IGNANVLIVFSKGKPSPKTVGHVAFHLPTMAALKKALAHLKKLKVQVEDPGDEIGPVA
                     PGSKSMGIWFSDPDGYRWELAVQSKK"
     gene            complement(3644..4234)
                     /gene="ogt"
                     /locus_tag="PK589_03400"
     CDS             complement(3644..4234)
                     /gene="ogt"
                     /locus_tag="PK589_03400"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF007626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNJ18670.1"
                     /translation="MSQQLHLLTETLITPIGTLVIMTDEQGQLRAVDWVDFEDRMRKL
                     LTRHYGLQGVLLTPAVNSTCHAAALSAYFEGNLHAIDALFTATAGTPFQKRVWNALRE
                     IPAGETVSYGDLARRIGQPTAVRAVGLANGANPVSIVVPCHRVIGADGSLTGYGGGLN
                     RKRWLLNHEKEAMAGSARFAAPSPTAAMVGCASFHR"
     gene            complement(4264..5733)
                     /locus_tag="PK589_03405"
     CDS             complement(4264..5733)
                     /locus_tag="PK589_03405"
                     /inference="COORDINATES: protein motif:HMM:NF017809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HNJ18671.1"
                     /translation="MELLDPDISYRALQTRDARFDGRLFVGVISTGIYCRPICPARTP
                     KREHCRFFSGSAAAQAAGFRACLRCRPEIVPELPGWRGTSNTVSRALALIAEGFLDHE
                     DGTVEALAMRVGMGARQLRRLFDQHLGVAPVAVAQSRRLLFAKQLIHETKLSMSDVAL
                     AGGFGSVRRFNHAFRSLYHRAPGELRRDTTAASVTLAATAPVMLQIPYRPPYDWDSML
                     AYLRARAIEGLEVVEENAYRRSVLHEGLTGTITIEHRPDKHSLMATIDFPSVRALQAM
                     VARIRHQFDVAADIEAIAAHLSKDVTLAPLIAARPGLRAPGCWDGFELAVRAVLGQQV
                     TVAAARRLGGTLVGLWGEPLPHAVDRRLMRTFPTARCLATADLSRLGMPAARRATISA
                     LARTAISYPQLFMPGGSVEERLAQLRAIPGIGTWTAHYIALRAFREPDTFPATDLGLI
                     RSARRLIGEQTSASHLLERTEAWRPWRAYAAQYLWTADQ"
     gene            complement(5844..6794)
                     /locus_tag="PK589_03410"
     CDS             complement(5844..6794)
                     /locus_tag="PK589_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ18672.1"
                     /translation="MNCTKCPTSTRAVIGLPRHNAAFCKACFTTFVHEQVARAIKAFK
                     MFTPEDRILVAVSGGKDSLALWHILLALGYQADALYVNLGIGAYSEESHRKVRHYADT
                     VAASHGAKLLVHVVEQEAGAGIRELATILHRPTCSTCGTIKRYQFNRAAVEQEYDVMA
                     TGHNLDDEAARLLGNVLHWQNDYLDKQSPTLPASVEGFAKKVKPLCRLSEREIAAYAV
                     VNRIDYIVEECPMAKGSKMILYKEVLNRLETESPGTKQRFYWGFLDKQTAPAPATESM
                     AEKDQRTLAPCDTCGQPTTAGTCSYCKMMAKAKTAAQPRA"
     gene            complement(6894..7091)
                     /locus_tag="PK589_03415"
     CDS             complement(6894..7091)
                     /locus_tag="PK589_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNJ18673.1"
                     /translation="MLVQLSHPHRQVEIKGPKRTKELLRELNLVMEAHLVIRGDELVT
                     EDEMLADADQIEIRPVISGGT"
     gene            complement(7225..7380)
                     /locus_tag="PK589_03420"
     CDS             complement(7225..7380)
                     /locus_tag="PK589_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18674.1"
                     /translation="MVNEQNLDWKNSSFSAVDVNNQKYKRLALASAMLFLGVVALLIY
                     QIIQNIK"
     gene            complement(7647..8762)
                     /locus_tag="PK589_03425"
     CDS             complement(7647..8762)
                     /locus_tag="PK589_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18675.1"
                     /translation="MRRASFPLISIGLGILLALLTMELVLRFLPVNEGARPQVVDHEH
                     PVRSLEPNRTFVWSADWNFAIVNVARTNNLGFVNDQDYLLDQPSPLLALIGDSYVEAL
                     MVPFRESVAGRLMTRSPSTERVYSFGTSSSALSNYLAYADYAAKQFHPQAMVFVIIGN
                     DFDESLLKYKNEPGHSYFVEDAHGTLALQRIDYHPSLWRRIIRASALVRYVTLNTDLL
                     NFVLKWQHLPFGRPSNHLAFVGNTGSKADPVRIADAQRAIDTFLTRVAEASGLPPHRI
                     LFAVDGMRPHIYNATTWQLTTGSYFDIMRQYFMEHARQQGFEVLDLQPRFAEHFAQHR
                     RPLEFATDNHWNSLGHEIVEEAIAHSDLYHWFRELAP"
     gene            complement(8858..9859)
                     /gene="galT"
                     /locus_tag="PK589_03430"
     CDS             complement(8858..9859)
                     /gene="galT"
                     /locus_tag="PK589_03430"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNJ18676.1"
                     /translation="MPELRRDPIVGRWVIISTERSGRPQDVHMPSAPLPSAALCPFCP
                     GQERLTPREILAYRPHASEPDGPNWTVRVIPNKFPALQVEGDMGREGLGLYDRMNGIG
                     AHEVIIETPAHKESLGDLPAKRVEDVLWAYRDRILDLKKDLRLRYILIFKNHGAAAGA
                     TLEHSHSQLIALPVVPTSVLEEIDGCKQHFQQKERCIYCDIMRQESSEGTRVVLENPE
                     FLCLTPYAPRFPFEMWILPKRHAGYFEECQRTQFEFLAPMLGEALRRMDAVLARPAYN
                     FILHSSPLHEKTGDFYHWHIEIIPKLTQVAGFEWGTGFYINPVAPEEAAKCLRDAVL"
     gene            complement(9961..12165)
                     /locus_tag="PK589_03435"
     CDS             complement(9961..12165)
                     /locus_tag="PK589_03435"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNJ18677.1"
                     /translation="MKTIQLCFLWHMHQPYYTDPLTGSASMPWVRLHATKAYYDMAFL
                     LEHFPEARSTFNFTPSLLLQLEEFSAGRVRDLFLEHAQRPAADLTPTEKAFLIRHFFS
                     ANWATMVRPFPRYQELLVKRGIDVHGQDLERLARQFSTQELLDLQVWHNLAWFGYGSL
                     QRFPRLAELRAKNRAFTEEDKQEVLALQQTAIRQIVPMYKALQDRAQIELTTTPFFHP
                     ILPLVIDSEFTRRARPDLPLPARFHAPADAEAQVRRAVEYHTRTFGRAPAGLWPSEGS
                     VCPEVLPIVAQAGIRWLATDEGILYRSLQMAGQGWNRHYHLYQPYAVGTETHPLAMVF
                     RDRDISDAFGFVYHKTTPEAAADDVLRRIRSLAYDIRLDNGTIAVILDGENPWEHYHD
                     GGERFLSLLFRAFESDGLQLGNGIRVTLNTMSDAMKAAPPTQRLEQLHSGSWINQDFK
                     IWIGHQEDNRGWDLLQHTRARLVELTPSLEPDKARAAWDELYAAEGSDWFWWYGDDFD
                     TDYKQEFDRLFRTHLRNVWTYAGMTPPEILNQPLVEARTPQGLDLVQLPLALISPTLD
                     GLASNFFEWRGAGTINPTPPLGAMWKSEGLFTAIFFGFDREQLYLRLDFDERSDTRQE
                     QCSADLYIGSGIQQHRLSFALKAGAADHFQLARADESGTYRDRGSYSTICRQKILELG
                     IPFKDLDIEVGAELRLTLTVSEHGMEIARYPHHSPATFTRPDDDFEATMWRV"
     gene            12316..12753
                     /locus_tag="PK589_03440"
     CDS             12316..12753
                     /locus_tag="PK589_03440"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNJ18678.1"
                     /translation="MASEQMVSTEIRGGSLVTPLSIMRYWGPVGLYAGLIFFGSSIST
                     PPEAISSLIKDISDKVLHFSEYALFGALIYRAARHGSGPWMAQHAVWAAVAGSALYGV
                     SDETHQLFVPFRQSDVLDVIADTVGGTVGAWGWRWIEQRTYRD"
     gene            complement(13316..14287)
                     /locus_tag="PK589_03445"
     CDS             complement(13316..14287)
                     /locus_tag="PK589_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ18679.1"
                     /translation="MQYVHLGRSGLRVSRLCLGTMNFGPHTTEADSGQIMDRALELGI
                     NFFDSANVYGWKVGEGVTEQIVGRWLAQGGGRREKVVLATKVYGRMGDWPNESRLSAR
                     HIKQACESSLRRLNTDYIDLYQMHHIDRDCPWEEIWQAMEQLCREGKVLYIGSSNFAG
                     WHIAQAQELAKSRHFLGLISEQSLYNLLERTVELEVIPACQAYGIGIIPWSPLARGLL
                     GGALSSPTSGRRAEEDVRKDIETHRQKLEAYEQFCRTLNTAPATVALAWLLHQPAVTA
                     PIIGPRTMEQLTAAISSLELTLTTEHLKTLDAMFPGPGGAAPEAYAW"
     gene            14441..15541
                     /locus_tag="PK589_03450"
     CDS             14441..15541
                     /locus_tag="PK589_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNJ18680.1"
                     /translation="MSTTNPAAPAAPLTPPDPERIAQTVATRLHFRGEMTALSALAGD
                     ASNRRYFRIFLKGTPSGLILMQLADPEGFKKSEEAVSGASAQITELPFINVLTHLQRT
                     GVTVPTLHYFDREAGLLYLEDFGDLTLAEACRGADPARLEALYRQAIDQLVLLQVKGT
                     TPPAPGCIAFHRRFDVPLLMWEFDHFLEYGITARLGQPMKPEDDSAVRAAFQRIAELL
                     AGQPQVFVHRDYHSRNLMVDGSRMGIIDFQDALMGPATYDLASLLKDAYIELDDAVVD
                     RLVEHFLDGLAGLGVKVNDRSAFRRVFDLTSIQRNLKAAGRFVYIDRVKHNPKFLADI
                     PRVLGYVKRNLAKYPELAPLRQHLAPYVTELQ"
     gene            15561..16301
                     /locus_tag="PK589_03455"
     CDS             15561..16301
                     /locus_tag="PK589_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNJ18681.1"
                     /translation="MKAMILAAGLGTRLRPLTDTTPKPLLPVAGTPMIVWNLLLLKRH
                     GIHDVVINLHHLGSMISQALGDGRALGMRIVYSQEPVILGTGGGIKQAEPHFNGEPVL
                     ILNGDTLFELDLGALMRFHREQQAAATLVLRKDPEAARWGLVEVTDRSEVVRITGRGR
                     ADSAQTLARMFAGIHILHPRLLRSLPAGKECSIIDAYVQGIQDGERIVGYDFDGFWSD
                     VGTPERYAQVERDAAAGRLNLSARSASN"
     gene            complement(16502..17878)
                     /locus_tag="PK589_03460"
     CDS             complement(16502..17878)
                     /locus_tag="PK589_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ18682.1"
                     /translation="MKAKVLIVDDDQDIVTMLQDRLDAGGYKTLTAGDGQRGLELIEQ
                     ESPNLVLLDLYLPRLKGMDVLKRMAQNKQCEEIPVIVMTAAGTIPDAVEAMRHGAYDF
                     LTKPLEKDHLLIVIQKALERDSLKRQVAALKSDVQNRYATIVGESQKIRGIIESAQRA
                     AKSDASILLLGESGTGKELFARSIHQWSPRQSMPMVVINCVALTETLLENELFGHERG
                     AFTGADRLQKGKLEMAEGGTVFLDEIGDMPLPLQAKLLRVLQDKEFQRVGGTRSVSVN
                     IRFIAATNKDLRQSVKAGQFREDLFFRLNVVSFTLPPLRERSEDIVQLAEFFLKRHAY
                     EAKRPGVTLSPAARAALTHYSWPGNIRELDNVLSRAVVLSPSDVIEPEFLALTPEDAA
                     GKSGADHALPYLNLTYHESMEAHSAYIIDRALEKAAGNQTKAAEFLDLQRTYLARLIK
                     QRKPAAAE"
     gene            complement(17898..19220)
                     /locus_tag="PK589_03465"
     CDS             complement(17898..19220)
                     /locus_tag="PK589_03465"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNJ18683.1"
                     /translation="MAHIIPFHGTLYNPTTVGDVRKVVAPPYDIIDSTLQKTLHDRHP
                     NNVIRLELGYEQAGDAPANNKYTRAAAALTDWLKSGALRKDSQPAVYYHTIEYRPPYS
                     APGTPTKVFKGFLSTVELEEFGSGKIYPHENTRAAAKTDRLNLLEACRANFSAIISLY
                     SDPQNEVLNLLEQSIASEKPRIDFQDDEGFRQQLWSVTDAKVLAKVVQIMNTKQLFIA
                     DGHHRYETALNYRKSRRQQAGAATSPQPYDNVLMLFASLEDKGLTVLPTHRVLTTPVP
                     ATKDLLGKFEPVFEVAALPLQAGNESQVRAQFIERLRSQGQSVPMFGLALKNDSNYYL
                     LTLRESHRPTASASPRDRLDVSLLQQHVIATLCPSQQEQEAMLYSKDDHEALNWVRQG
                     KGTAALLLNPTKVAEVKAVASAGERMPHKSTYFFPKPLTGLVMNVMEG"
     gene            complement(19491..20495)
                     /locus_tag="PK589_03470"
     CDS             complement(19491..20495)
                     /locus_tag="PK589_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNJ18684.1"
                     /translation="MSNGPTTLTLHSMADVWDVYREELEGVEEQIRKNLDSSVDLVNT
                     VAAHILNSGGKRVRPLFVLLSAHLCGYAGQDHQALGSLVEFIHTATLLHDDVVDDADL
                     RRGRRTASKVWGNQISILVGDYLYSRAICQIVDFRNQGINEALSEACRKMAEGEVLQL
                     YYNGNPLMPEPEYLRIIEHKTAGLIAASCKIGAIVGGATEELQEALFRFGQRLGIAFQ
                     LADDTLDYTANGEHLGKTLGQDLRQGKATLPLLHLLQHCPEPDRQLIKDRMETRTLTD
                     EELRRIVALMQEYGSIAYAMERAHAFVAAAKRDLDLFHDNTAKRALSIAADYMVTRDR
                     "
     gene            20731..25251
                     /gene="gltB"
                     /locus_tag="PK589_03475"
     CDS             20731..25251
                     /gene="gltB"
                     /locus_tag="PK589_03475"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNJ18685.1"
                     /translation="MNVPGLPPKQGLYDPQHEKDSCGVGFVVDIKGQRSHQIVQQGLQ
                     VLESLTHRGAQGCDPCTGDGAGILLQVPHEFFKRAAKDSGIKLPGAGEYGVGMVFLPP
                     DADARAQCETVFTRVIKDAGAKLLGWRDVPVKSDAIGPVARSTEPFMRQVFIARGIFT
                     DEEFERRLYVIRKCAERAVRESAIEGRDYFYIPSLSSSTIVFKGLLLPHQIPQYYQDL
                     TDSSLTSAMALVHSRFSTNTFPTWPLAHPYRYICHNGEINTLKGNVNWMRARQGRLNS
                     ELFGEDMKKLFPIVYENQSDSASLDNALEFLVLGGRSLPHAMMMLIPEPWVANPQMDL
                     DRRGFYEYHAAMQEPWDGPAAVCFTDGKLIGATLDRNGLRPCRYQVTTDGLVVLASEA
                     GVLPMDPQRIRQKGRLMPGRMFLVDTEQGRIIDDEEVKADIVRRKPYRSWVTEHRISL
                     DELPDPINVPQPDHPTIRQRQQAFGYTIEELKMVITPMVVEGQEAISSMGTDTPLAVL
                     SERPQLLFKYFKQLFAQVTNPPIDPIREELVMSLTTSIGPKPNLMDEHPESARRIRVK
                     QPILTNADLHKIREIADPHFKSKTLKMLFRVAEGPEGLGAAVDDLCRQASQAIREGYK
                     FLILSDRGVNAEWAPIPSLLGVAAVHHHLVRECTRTEVGLTVETGEPRDVHHFACLIG
                     FGAGTVNPYLVFESLVDLERDGYFPEGLDAATAEGKFIKAINKGLLKIFSKMGISTVQ
                     SYCGAQIFEAIGLNHELIDRYFTGTPSRIEGIGIREIGEETLRRHRVAYEPVPIRQLD
                     FGGEIHYRIQGEHHNWNPDTIYKLQHATRNNDPKTFAEFSQLVNDESKRRSNLRGLLE
                     FKFLPEPIALNEVEPAKDIVKRFTTGAMSFGSISKEAHETLAIAMNRLGAKSNTGEGG
                     EDPERFEPLPNGDSRNSYIKQVASARFGVTSHYLVNAKELQIKMAQGAKPGEGGQLPG
                     HKVDENIARLRYSTPGVQLISPPPHHDIYSIEDLAQLIFDLKNANADAAVSVKLVSEV
                     GVGTVAAGVAKAHADKVLISGDSGGTGASPLSSIKYAGVPWELGLAETHQTLVLNDLR
                     GRIRVETDGQMKTGRDVAIAALLGAEEYGFATAPLIIEGCIMMRKCHLNTCPVGIATQ
                     DPVLRKKFTGQPEHVVNFFFFIAEELRQIMAKLGFRTINEMVGRVDKLKIHKAVEHWK
                     AKGLDLAPLLKMPEVGPEVPRYCVQKQDHGIAEILDRALIEQCAPAIDRGQKVTLELP
                     IRNLNRTVGTMLSSQVSKKYGQDGLPADTITIKFNGSAGQSFGAFLSRGITLILEGES
                     NDYIGKGLSGGKIIVFPPKNAIYTPEETILVGNTSLYGGTQGEAYFYGMAGERFAVRN
                     SGVRAVVEGTGDHGCEYMTGGVVAVLGRTGRNFAAGMSGGVAFVLNELDKFQSRCNLG
                     MVELEPVVSDEDKRLLHDMITSHFLYTGSRNAKRILDGWEAILPKFVKVMPIDYKRVL
                     AERKAAAAKVQK"
     gene            25331..26764
                     /locus_tag="PK589_03480"
     CDS             25331..26764
                     /locus_tag="PK589_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNJ18686.1"
                     /translation="MGDPKGFLKYAREGPKRKPIELRVLDWKEMYEPIPEEKLKVQGA
                     RCMDCGVPFCQGNTGCPVVNLIPEWNDLVYRGRWKDALKALHTTNNFPEFTGRLCPAP
                     CEGACVLGINSDPVSIRVLEWNIIDRGFNEGLVEPALPVRKTGKTVAIIGSGPAGLAA
                     AQQLARAGHSVTVFEKADRIGGLLRYGIPDFKMEKWVIDRRLEQMKAEGVEFKTGVTV
                     GKDVTGEQLRQEFDAVGLTMGAEMARDLPVPGRELKGIHFAMEYLTQQNKRTAGISVS
                     EEPITAKGKRVVIIGGGDTGSDCLGTAHRQGCSEAHQFEVLPEPPPSRSSSTPWPLWP
                     MQLRTSHAHEEGCDRQWSVSTTKFTGHNGHVTKLHANRVKFEGGKFVAIPNTDFELDA
                     DLVLLAMGFTGPVRNGFLDSLGVNYDARGCVTVNENFMTNLDGVFAGGDTKRGASLIV
                     WAIAEGRKMAAGINQYLQANKSAKSGR"
     gene            27954..28391
                     /locus_tag="PK589_03485"
     CDS             27954..28391
                     /locus_tag="PK589_03485"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNJ18687.1"
                     /translation="MTQQTYPRSPKVLLGGIAHLGRFIDKVRLRHAGKIQDYNYITVG
                     FDKYLIDFLQIDAKAFEQQVLAGESDEKLLSWVKTHGRKHSEEEIAQWSKGLLAGGPK
                     DDAAKQRFQGRVQDIATKRGVPLTSLPPVTTWADVIELDEERM"
     gene            complement(28420..29061)
                     /locus_tag="PK589_03490"
     CDS             complement(28420..29061)
                     /locus_tag="PK589_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18688.1"
                     /translation="MHRSIALLLFVITTSTASLLVTAEPSHAQGGGSQVTITPTDSKV
                     IAWSAAGGGVEESLQPNETVISSAARGHTGFVQTSSRLLGFSAGLRQWREVSIGVEER
                     IGRHQLLPFLILAHSNQQVFGFQETRGHWTTIALGPNETVSQLRGQGHVAVAITNERA
                     LGFSTYTGGFFNIDWTPNERVLSVDGSQNGMVVRTSSRTVIFKSQSTGWTEVR"
     gene            complement(29223..29714)
                     /locus_tag="PK589_03495"
     CDS             complement(29223..29714)
                     /locus_tag="PK589_03495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNJ18689.1"
                     /translation="MTRVSLAGLLILCAIVLLSRPLHAQTGSPTIEDGSKVTLQMTIT
                     LPNDHVMIPPQTSEYTHGEKQLLPGLEEALVGLHSGDTKRVELDADHAFGQYDAGKKM
                     TVPRAQLPHTIARGDMATTAEGRPFTVVSLSDESAVVDFNHPLAGKHIVLDIVVLNVK
                     PKA"
     gene            complement(29770..30051)
                     /locus_tag="PK589_03500"
     CDS             complement(29770..30051)
                     /locus_tag="PK589_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18690.1"
                     /translation="MKSIIVIGSLVCVLAMPIVAPLESQASLTYTRVKVESVDQASQS
                     LTFKTAEGEVWTLAALSADMLSGLHKGDTCSLEIDFDNRVTKIVKIDSP"
     gene            30263..31498
                     /locus_tag="PK589_03505"
     CDS             30263..31498
                     /locus_tag="PK589_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18691.1"
                     /translation="MSQDDASFPGSESGGITVALALSAIVTLPLMGPWLSGQSLRPYL
                     EFPPLSTGVVTQVAFSWPVFVGLSALIVCLLAPFIVRVARTNSRGVSTVAGQERTPYG
                     SVRRQCVLPHAFPWWGWGAVAWTICAWFLAWTRFEWMQPWQPHTFTPLWLGYIVVVNA
                     LTYRSTGRCMLIHRPRYLLSLFPLSACFWWLFEYLNRFVQNWYYVGGGTLTTGEYLVR
                     ATLPFATVLPAVLSTAEWLTACPRLSAGLDHVVTINFSRRVWGWLLLSGAAAGLLGIG
                     LWPDYLFPLVWVAPLLVITALQLIRNEPTIFSETAHGDWRTLWAVALAALVCGWFWEM
                     WNFYSLAHWQYAVPFVQGVTLFHMPLLGYAGYLPFGLECWAVADLCLARKFAGGVAYY
                     RAAEPETDFAQRNPTVAER"
     gene            complement(31435..32379)
                     /locus_tag="PK589_03510"
     CDS             complement(31435..32379)
                     /locus_tag="PK589_03510"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ18692.1"
                     /translation="MIPRTLFPGIASLLHALSLVILSVAATWLNPALSIAARAPTPPP
                     TAELAKHLTSIAELAASSPAVLIPEGPFLLGSIRVDDDPYGMGTQFDDTELPQHRVWL
                     DAYEIDRDEVSLGEYLTYLHQRKIHPSKDLQHLIWHVITVHSVSDETLAQWPALYVTW
                     KEADNLCRASGKRLPTEAEWEKAARGPDGNRFPWGESLPDNSLAMFGQHHVHEIPILA
                     PVSSGEPGKSYYGVHHMAGNVAEWVNDWFGFDYYAYMPERNPPGPTSGRYKSLRGGSW
                     KSRPIMLRTATRSGAPADQRSATVGFRCAKSVSGSAAR"
     gene            complement(32379..33134)
                     /locus_tag="PK589_03515"
     CDS             complement(32379..33134)
                     /locus_tag="PK589_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18693.1"
                     /translation="MIKASQRSLAPLFSLAVLSWCVGCTPDESPFRQENENLRKQLAK
                     QESLLNSLQDGNKVMQQQIDLLNQELRDAKKQTEHAQAEAKSLQTEAKTLGTKLDAQL
                     AESKKLSGEIQRTAAKAAQATDSIRVEEKGSQVEDLPRPLPAVAKAAEEALTRNGYRI
                     RVTVKTDQKAVFVTERKISIPTSVETAGSRNQYVVSLQALPSNVTRLSVKAEFERVAQ
                     GGRVLAVDAEEIAEIERRLIAEIGKAVASPSKL"
     gene            33274..33903
                     /locus_tag="PK589_03520"
     CDS             33274..33903
                     /locus_tag="PK589_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18694.1"
                     /translation="MSLALFQPAAAAVSIAAMAEPAEPVPAEDYAFFDLAVEDKFLTS
                     HTKLIVIERMTATHLQPDEPQLPTLAWFAEREWFDGRLPQDLVRDFVGKNQRPSRLDT
                     RFSFGVRYRFMMGEGGGDAEASVPLIPATWLVQEEGGAPDVIDHLAFSRVGLNLRADQ
                     ALLYVANPRPDGTGAGFLLWFVRQQKVWSLHDTEVLWVSRPDQGQSGRR"
     gene            33951..34580
                     /locus_tag="PK589_03525"
     CDS             33951..34580
                     /locus_tag="PK589_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ18695.1"
                     /translation="MIRKTFSVPPLGCNCSIIGDPVTKQAIVVDPGGAPERILQEVRA
                     MGLTVVSIVHTHAHFDHFLASGAMKQATGAALCLHPDDLPLWDILEMQCRMFGVPYVP
                     VPQPDYWLKDEERVAVGEMEGVALHTPGHTPGSMCFHFPAAKLLVAGDTLFRGGIGRT
                     DLWGGDFDAIEQSIRERLYTLDADTAVVTGHGAETEIGLERETNSFVRA"
     gene            34698..35213
                     /locus_tag="PK589_03530"
     CDS             34698..35213
                     /locus_tag="PK589_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNJ18696.1"
                     /translation="MIATARRWLVCPLMSFLLGFSAADVLVYADEPGSSPQVTISQVR
                     VGLSDHGPVVLLSADGKTIPVFVDHTVAASIQAALTGEKLPRPLSHELMHTILESLGG
                     RVVRTVITLKAGTYYGSLTVAFQGQEKVFDSRSSDSIALAIHFHAPILVARDMLDAVG
                     KSSLEPKPESL"
     gene            35354..35677
                     /locus_tag="PK589_03535"
     CDS             35354..35677
                     /locus_tag="PK589_03535"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ18697.1"
                     /translation="MKNQRKHERVHVQFRSHFSMKGKMLAGDGDLTDLSPGGCRIISP
                     VEVLVGSEVELCIFPGDNANPILIDAATVRWCRPNEFGLSITKIRGPVQRQLTDVWRK
                     LAKPA"
     gene            complement(35928..36917)
                     /gene="trpS"
                     /locus_tag="PK589_03540"
     CDS             complement(35928..36917)
                     /gene="trpS"
                     /locus_tag="PK589_03540"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNJ18698.1"
                     /translation="MTTTRKRVLSGMQPSGLLHLGNWLGALENWNTLQAQYECYFFVA
                     DWHALSSNYADTSRLREYVRELLIDWLAAGIDPNRATVFVQSQVPDHAILHLLFSMII
                     PVSWLERNPTYKEKQEEIKERDLSTYGFLGYPVLQAADILLYKPDFVPVGKDQLPHLE
                     LTRELARRFNSLYGSVFPEPQEHLTKFPKVLGTDGRKMSKSYGNAINLSDTEAVVRQK
                     LKTMITDPARVRRTDPGNPDVCPVYDFHKIFSPLPVIEQVNQDCRKAAIGCIDCKKLV
                     ADRVVERLAPMWEARANLTQHPGRLDDIAEDGRRRATAVSSQTMAEVREAMKI"
     gene            complement(36972..38969)
                     /locus_tag="PK589_03545"
     CDS             complement(36972..38969)
                     /locus_tag="PK589_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HNJ18699.1"
                     /translation="MVPRSVSRAPHLRACSVALAVALLVGFATLGETAPAPKTPAAPR
                     SNQAKPPANTGQSAIDQAKRLIDAEQPEAAAVMLRRFIEGGPGPDLLDDAYLLMAAAM
                     YGMKEHAETIRYVNQLLGEFPNSDLVDRAKLLLAKTHARAGNLDLALPLLSEVRSLSA
                     DPAIKRDALRLTGEFQVQKKDFLRAIQAWLDELPLDVGDQSRETETQIRELVNDKLDA
                     PTLMRVRDAYPKTFPGDLASIKLIELHTAAGEDHLVERDVRLFLSRFPNHPYAAKAAD
                     LQSVVRTKFKSHAYSIAAIFPMSGKLAPFGTEVLNGIQLALERTKDGGETPSIGLIVK
                     DTESDRAAFLDELSAVLSDDRPLAVIGPLLSKNLPVMAEMAERTHIPLITPSATVPNL
                     RRFGNYVFSTALTYGHQAKRVADYAVKEQQFKRFAILYPDTPYGRDLARLFAQEIRQQ
                     DGEVIVSEAYKEGDTDFRAVIGRLKAEDLKKYGVEVQVDNDPAKTGIRQGAGKKGKRL
                     LYSPGFDAVFIPGRSLDVGLLAAQLAFYDIAVPLLGTNGWNTPDFARVADRSVEGSVF
                     TDGFFTDSTSPIVQEFVERYRKRFQATPSLFAAQGYDAARLAVEAIRRGATSGEAVRD
                     YLMMQHDLPTLSGPSGFNPDGTLNRHVFLIQVKQGKFVPLE"
     gene            39130..40083
                     /gene="xerD"
                     /locus_tag="PK589_03550"
     CDS             39130..40083
                     /gene="xerD"
                     /locus_tag="PK589_03550"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNJ18700.1"
                     /translation="MKSRDAASHAPQESDSLPLAPLVARYCDDLRIARGLSRHTLSAY
                     QRDVTEFQRYLRDQQIHDPAQVSPPLLAAFLEHLHRGGLAASSRARALAAVRSLFRFL
                     KQEGVVAANPTVSLRSTSRARHLPKTLSLEEVASLLDLPHRGAPEDHRDRAMVEVLYA
                     AGLRVSELIALRMDQCNLEVGYVGITGKGDKQRVVPIGRPAVAEVQAYVMSARPMLLK
                     HRSSRFLFVTRRGTPLTRQAFWKLLRARAQRAGIARMPSPHMLRHSFATHLLQRGADL
                     RSVQAMLGHADIATTQIYTHVDAAQLKKIHTACFPRHKSRR"
     gene            40227..40302
                     /locus_tag="PK589_03555"
     tRNA            40227..40302
                     /locus_tag="PK589_03555"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40260..40262,aa:Val,seq:tac)
     gene            40436..40513
                     /locus_tag="PK589_03560"
     tRNA            40436..40513
                     /locus_tag="PK589_03560"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40471..40473,aa:Asp,seq:gtc)
     gene            40586..41302
                     /locus_tag="PK589_03565"
     CDS             40586..41302
                     /locus_tag="PK589_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNJ18701.1"
                     /translation="MFQSGVMGLVGSLGAVSKIVLLLLLVASILSWGVILYKWRTFKA
                     ADREDQRFFNAFTKIRDLDELYRQSKRAEGSPSARVFQGIMDRVVTASGDGAVSPSLA
                     AAGSKESAAAFDHQYMDKTVSYLVQNQVSHLEAYLPVLATTGNITPFIGLLGTVLGII
                     DSFREIGMQGTASIAAVAPGVSEALVATAAGLFTAIPAVIFYNYYLTRIRKTVFRIES
                     FTVEAMRSLQTRLKQPQAGV"
     gene            41305..41757
                     /locus_tag="PK589_03570"
     CDS             41305..41757
                     /locus_tag="PK589_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNJ18702.1"
                     /translation="MTSETRHRRFMAEINVIPLVDVVLVLLVIFMVTAPMLYRGMDIN
                     LPKSASNTIKPEARAVLSIERDQRLYLDKDAVSVVQLERKLRALKDQSPDVSLYLRAD
                     RDVPYGIVVQVMDSVKKAGIEKLGMVTEPTGAERVNESVATPSQPRKK"
     gene            41850..43040
                     /locus_tag="PK589_03575"
     CDS             41850..43040
                     /locus_tag="PK589_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNJ18703.1"
                     /translation="MGEAGGDRFRKTVVVSLLLHLCLLSIIMGAKFFKKTERPLSAVE
                     VSLVTLPPVEAKPEPQVEKVEKAVPKPAPKPVQSAPIPVPPKPVQAPTPTPPPVQSAP
                     VAKPAPPAPAPAPAPVPVPKLEAPVLTAPQPIPQAAKSPSSAPVTNRADVLRDVMKDV
                     ELPLNAPKYGDIAPMKPAEVKKSAQAKLGAPERSDLDAMLKKLNVPDMAAPQVAVPQE
                     PPKPNVTPAKRASLSEEMTSDLDRELQDLKKLQAPPPVKATEPVREVKPMFREPQPPV
                     TAPPVAASVTRSKPTPMLRVPGMSGSNPYLARVQARISGFWTAPPVDISGKALTVTVR
                     FRLERDGRVGSVIIEQSSGNDYYDMAAQRAVQSAIPLPPFPPDLTDSYFDAHFTFAVG
                     EAAG"
     gene            43037..44368
                     /gene="tolB"
                     /locus_tag="PK589_03580"
     CDS             43037..44368
                     /gene="tolB"
                     /locus_tag="PK589_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNJ18704.1"
                     /translation="MNRVIIGLMLTLCVVVGAGLFGILDSRATDVFLEATRPDFQKIP
                     IGVFGFQNGGGPEWLGGRLEEVLKADLQRSLVFSLVDLPGIGVKAREVSTTDKAVFKQ
                     AAENGVSVLVWGKSGQKNGSKDSELLMDGFVYDSGSDEVVGGKRYVGSTSVVRLMAHR
                     FADELVFRYTGEPGIARTKIVYVAEHGNARELFVMDYDGYEPKQITADGFLNLMPRWS
                     PDRRFIVFTAYRSRNTQDIDILELATGKRWTLVSMPGLNITPALSPDGNYLAFASSQD
                     GNSEIYKLDTRTKTPQRLTVNQGGDLSPTWSPTGREIAFTSDRGGAPQVFVMSADGSN
                     VRRLTYEGDYNAAPAWSPRGNWIAYVCRTAQRLYKLCIVSPDGQKRVQVTTGPGIDDS
                     PSWSPDGRHLTFSSTVDGKSHIYMVDTDGKNLERITFGGTHNSSPSWSPAL"
     gene            44436..45185
                     /locus_tag="PK589_03585"
     CDS             44436..45185
                     /locus_tag="PK589_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ18705.1"
                     /translation="MRIRVATMGLTMVVGMLLVMQGGCSKKSIQSGGDAQSSDRGMAK
                     SGAPAQSQAQLPPPTGLDAPSATFPDLSLSSKPEDPETGGLRGFDSVSGGKAPSEERL
                     GGGGTMLAKVEPSESTARQIEEIRREQAKEQAASAEAGLRDVFFGYDSWTITEDGRQS
                     LTQDAQWIKANAGALVKIEGHCDERGTLAYNLVLGEKRAKAVRNYLVELGVGANRLSV
                     VSYGKERPFCNERSESCYQQNRRGHVVVRSK"
     gene            45251..46927
                     /gene="ybgF"
                     /locus_tag="PK589_03590"
     CDS             45251..46927
                     /gene="ybgF"
                     /locus_tag="PK589_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNJ18706.1"
                     /translation="MDSEWPYRKTMTDQPSNAKRVTPGTYAQAARFGACVVVVTGCVL
                     LSGCVAQQADLKQTERELQRRIKQQTEEQAQNRARQNQEIVSLREQDIPSLRGDVDKA
                     LHRAQSLDARQDDLLAKVASQESKFERRIGEAEKRSAEEGKRLGWVEKQLVDQDAMLK
                     GERDRNRAELAAVTARLDQINSHIEAIQKNVLDAMQKTTTGLAQKVDSRLDEQQKLLH
                     GLETRSQNVAQLDAQNKVLGDQVGKFNQALVDFKQALGSLGERVVQQDQTVKHLAATL
                     EQDTVALSKRTDALAGKMDADNKVTADHFNEVNRSVASVAKALENAGGKFVSREDDHE
                     RRLDDTTRELTHVQAQIQTLDKNLENQHAFLKQVEQHLVALRANATQRSEPAPAVAEA
                     APLPQAIPAPVPPPAPAQTQDFASSSAITPRAENRSAALVADRESYERTLTRFKDGDL
                     DGARQGFAEFLVQHPHSDLAPNARFWLGESYYGKKDYSRAIDAYDQVQLNHPASEKVP
                     AALLKKGYAYLALKDRKKAASALKQVIDLYPRSPEANKAMDKLNQLKESH"
     gene            46930..47709
                     /gene="ybgF"
                     /locus_tag="PK589_03595"
     CDS             46930..47709
                     /gene="ybgF"
                     /locus_tag="PK589_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNJ18707.1"
                     /translation="MKLRFAGALRVSAMLACGLVVAGCAKHADFLEIRDQVSIMARTQ
                     DQEQKRFEAMQRRLESLERVREPEGGKLRLDDAMTRLQKLEGRLAKVEEAQLAQAAAI
                     RSDAALAEASRQARISKPSGPIEPQAIVPGVPSITPTSAFNLAYNDYLNGKFDLAVNG
                     FQHFIKDFPSTSLTPNAHYWLGESYYGQKDYIRAIQSFEHVVNEYAGNEKVPAALFKL
                     GLSAAETGDTAKSRKYLKRVIEEYSTSDEAKLAKTKMAEIR"
     gene            47782..49578
                     /gene="mutL"
                     /locus_tag="PK589_03600"
     CDS             47782..49578
                     /gene="mutL"
                     /locus_tag="PK589_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNJ18708.1"
                     /translation="MDIASSVGKIQVLPSDVIGRIAAGEVVERPAAVVKELLENSLDA
                     GSSAITVEIKDGGLGLIRVSDDGEGMSRRDASLAFERHATSKLQSDDQLGAVRTMGFR
                     GEALPSIAAVSKVRLTTVARHDQVGTQLWLTGGTLTRVEDAAAIPGTTIEVSELFFNT
                     PARRKFLKSTTTEFSHISHVVQQAALAWPQVYMRLVHNGYEVFALPAVSSPRDRVLQV
                     YRAAFGERALAVDVERDGLLLKGFIIDPVRARAGRTPQELFVNRRPIKNSTVQHAVID
                     GYSSFLAKGHAPLFVLFLDVEPHRVDVNVHPTKREVRFADTEQIHQLVRSAVRQTLGR
                     AQVEASMAGAAHAEMTGVIPPTLSFPERERMSFGEAPPGPMARSKASVTPPDSAESQT
                     SFVGEGATAYASPASTDIVALGQMNRTYLIAQVGNELQVVDQHTAHERVLFERLWRAW
                     QGRAVTSQPLLLPEPLELPVQQAVILQRHLPELERLGLLIEPFGPSSFLIRSLPIMLG
                     HPDLSALVQDLIDDLEQWDSISSLETKVKPILASLACHAAVRAGRSMALPEITQLVQD
                     WVAEGLIMTCPHGRRVAFRLSGDELARLFDRA"
     gene            49598..50626
                     /gene="miaA"
                     /locus_tag="PK589_03605"
     CDS             49598..50626
                     /gene="miaA"
                     /locus_tag="PK589_03605"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNJ18709.1"
                     /translation="MIRLTESVLASRPLVVLVGPTAVGKSDVGLRLARALDTDLLTAD
                     SRQVYRGMDIATDKPAVAQQQGVPHRLIDLVDPDESFNAGQYRQLALQEIERLYGERR
                     LPLIVGGTGLYVRTLIHGLCDAPRADQAFRDSLLQKAQGQGRFFLHAELAQVDPELAA
                     RLHPHDEVKIVRALEVFHLSGRRLSDVQQEHRFAEQPFSVLMIGLNRDRAELYRRIDE
                     RVEAMFARGVVEETAALLAKGYGRETGAMKGLGYQQVAGYLSGDYDRAEALRLLKRDT
                     RHFAKRQLTWFRKEPGLQWWMLTEQDTAEDVAGRLLETIHTFVQALNDERQPHRPAPL
                     TIEMESTS"
     gene            50623..51522
                     /gene="mtnP"
                     /locus_tag="PK589_03610"
     CDS             50623..51522
                     /gene="mtnP"
                     /locus_tag="PK589_03610"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNJ18710.1"
                     /translation="MKKAKKTIQPAVGIIGGSGLYQVEGLEQVREVRLRTPFGAPSDA
                     IVVGVLGGMGVAFLSRHGRGHRINPSSINYRANIYALKSLGVTQVISISAVGSMKESI
                     EPGHVVLPDQFIDLTKRRISTFFDEGIVAHVGFSEPVCSSLADTLEEAGRSAGATLHR
                     GGTYVCMEGPQFSTKAESRLYRQWGVDVIGMTNMPEAKLAREAELCYATMALATDYDC
                     WHETEEAVTVGAILATLHRNVALAKEILKTAVPKLDPDRACACRQALATALITAPDKM
                     SASAKRRLNLLIAPYVRTRKGKR"
     gene            51526..52443
                     /locus_tag="PK589_03615"
     CDS             51526..52443
                     /locus_tag="PK589_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNJ18711.1"
                     /translation="MGKLLVVGSVALDTVKTPFGEVAEVLGGSATYFSTAASYFTSVD
                     LIAVVGDDFPEQHVAFLKSRKIDLAGLERRPGATFRWKGAYSHQLNEAQTLDTKLNVF
                     ETFRPKIPAQYSSPDVLFLGNIDPNLQLDVLQQVKRPALVACDTMNFWINGKRDALWN
                     VLQHIDILIINDGEARALGEDTNLVKVAKKILARGPKHLIVKRGEYGVLMFNDKQVFG
                     APAFPLDDVRDPTGAGDTFAGGFLGYLAATGNRSPEAMRQAIIFGSVMASFTVEAFSL
                     DRLRILDYKEIEARFKEFKRLTHFEDIGA"
     gene            52440..53138
                     /locus_tag="PK589_03620"
     CDS             52440..53138
                     /locus_tag="PK589_03620"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ18712.1"
                     /translation="MIVVDRQETRSCRASGMVGSSCLVFLLGLSVLGGCANEENLRKS
                     KGFYQEGVARLSSDQQQAYVSFQKAVKLNPDNKEAHYGLGHIYASQGRLKLAEESFRE
                     AIRIDGDYAEANTYLGQVLASQDRWKDAIAAYRQALSNPLYPTPDLARFQLGKALMHE
                     GDLQGAMEVLEDATTVTPPNVPPAMLQLELARVYHKLGFDARAREALSKVSTLDKGGE
                     QAAAAQELLGKLKP"
     gene            53148..54095
                     /locus_tag="PK589_03625"
     CDS             53148..54095
                     /locus_tag="PK589_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNJ18713.1"
                     /translation="MESVGEFFRQVRETKGLTVDEVASKTRIRTDFVKALEEGNFAKL
                     PDQVFARGFVRSYARSLGLDEEDAIHRFVQSAGAFYEKQGERERLRQRQVEEERRRRA
                     NRKAVGVAIAVAIATLVFLLSREQTSTLVRRSGPDAPTVSKKVAPFAKDAKEAGSRPA
                     AELPPPIAPPLKPAEPAVVPAKPAQDKPAPVAQAPAPVAVAKAPLPSDTVVTQPSASM
                     GSDGPLAGLSVDGPVGSDGPLVLDLAATELSWVVVQVDSGSHQEALLRPGEKAQWKAQ
                     DQFTVTLGNAGGVRAELNGKPQKPFGPSGKVVRDVVIKR"
     gene            54544..54996
                     /locus_tag="PK589_03630"
     CDS             54544..54996
                     /locus_tag="PK589_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ18714.1"
                     /translation="MGYFSKFVGVCAAVTLLSVAVVGAEEKDPLKPRVAPDQMADAKA
                     MKNPVASTPESIAKGKALYEGKGTCFNCHGKEGKGDGPAGAILNPSPRNFTNCKFHKK
                     RKDGELFWVIKNGSPGTGMVSLVPAAITEEEAWTIINYERSFCKGGDE"
     gene            complement(55192..57054)
                     /locus_tag="PK589_03635"
     CDS             complement(55192..57054)
                     /locus_tag="PK589_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18715.1"
                     /translation="MKTNRVVLVGIALLSLLASVGCESIKSRFSRQALPDLGPPVPLT
                     VHMEIDPSLSKAKTEYIDNCSRIHAFSIGPTVEDTLIQAAHQTFRTVTIPGSQAAGGK
                     PDVTIRVRMLDPRFKLQTDALYDRAPAELSLDALAEFFDASGAPLGERPLQATRKERL
                     QLELTQQRCDYIVDPLLQDTSTMLATQFMQEARVLLAPATPPPAAATTATPTEPSSKA
                     TAVPAAPAATPVQPLSFKATLLDENGNLIIESGERIRVRVDVVNTGTQLVQDSTVKLT
                     GPQTVLTQFPATTLATGTIPAAGSKSLEFVATMPQSPSLQRAELQVALLNASGQAAAP
                     PQTLPLSFQPTAALLEDVDHIPAATSGAQRPGDYLLAIGLSSYREQDIAARKYASLDA
                     ETVAAYFQTLGGLPQNNVRLLRDWSALRPDIEEALLDWLPAKTTKESLVVVYFAGQAM
                     VNAAGDTFLIPYDGSMGSPTRLYPLKELEAALGRLKAKQVLFVFDGTVLKNGADGRTK
                     AAAPKWLGTSGSVLHMIATTGFGKSLESETWHHGLFTYYLLRGLRGEADLNRNGEVTI
                     AEVTAYINRKVPGAARSTFKQEQQPQILPTLRGSEKGLDTVLTKPTMTFPSDQP"
     gene            57267..59000
                     /locus_tag="PK589_03640"
     CDS             57267..59000
                     /locus_tag="PK589_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNJ18716.1"
                     /translation="MVVLGLSNMRDAAAALVADGRIVAAAEEERFVRQKHVTALPVHA
                     MQYCLREAGMALRDVDAIAVPWKYWQVGRRLRLAVTAMLRSTQLFRVKGTRSLERASQ
                     EWKELFRLQQELSTRVEPGAGRPVFLDHHLCHAASSFLVSPFERAAILVVDGASESDT
                     TLLATGEGNQISVLERTPLPHSLGQFYAAMTAFLGFRPDQDEYIVMGLASSGEPSFAP
                     TLSREILRVLPDGRFELNTRLLDFHLARAGHFVREFIRLFGPPRRPQDEITQRHRDLA
                     ASAQFVLEETLLHLGRRLRSLTQATSLCLAGGVAYNCVANGRLRAELGFDQVYVPPAA
                     GDSGAALGAALWWTARRGAATSKTVMPDAYLGPQYDESACRAALSKTGLVAEHLADDR
                     LYERVASELAHGRLVFWYQGRMEWGPRALGNRSLLADPRREDMRELINSKVKCREAFR
                     PFAPSVLAEHAEEFFHVPAPSAFMQFTVRVKASAKGLLPAVTHVDGTARVQTVTREAN
                     PRFYDLLATFGRLTGVPVLLNTSFNVQEPIVCSPDEAVRCFQRTQVDWLVLGNLLVGR
                     PSPMPPQHHEG"
     gene            58990..60525
                     /locus_tag="PK589_03645"
     CDS             58990..60525
                     /locus_tag="PK589_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18717.1"
                     /translation="MKAEPVRGSFPLPALMLILDVLLGAFVLNLVLQPLVEPDLGWHL
                     RAGLDLIAQGWRLPDTDPYSHTMPDWHWVEHAWLTDGLLGLIYRMLEPSGGLGLIAFF
                     GGVTALAWWIAAGVASVHRTCRLAAMVASLWVALPFLGARTQLISLLGVAVVLTIWNR
                     MQRGQPQWVWALPPLFLLWANLHGGFTAGLFLITVLLSLSFLVKICVEWKVVQAPRDE
                     PVLSWDVIRLCAFAILVSFAVTCLNPYGWRLYVEIYESLTDRFMIDTLREWQPVSFHG
                     WAGRAYGFYLLGFIGLMAGWYRRVEPVRWVLLALMLGLSLLHWRNVTLFLIVSLPLAA
                     ELGAAAADSFLRRVPVLQAHAAGTVLVVSIMAATALYALGTEHLEHVWQSGRTPDRYF
                     ERTEYPIEAVRWIRAHRDETGTRLYNDYSYGGFLLWQLPGERIFIDGRMPAWRIKDRW
                     IFHDYVELNREDSPQLALLDRYGVDWAMIPRGSALAVALEAHPAWKNVYADAKVVIVR
                     RQS"
     gene            complement(60542..61357)
                     /locus_tag="PK589_03650"
     CDS             complement(60542..61357)
                     /locus_tag="PK589_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ18718.1"
                     /translation="MTLCSTQTPSLSVIIPAYNEARRLPLFLQRVIAHLDQRGQFYEI
                     LVVDDGSQDNTAHAVRMAAHHCPHLRLIQLTCNMGKGAAVRRGMQAARGTIQLFADAD
                     GATPIEELGRLEAALAAGADLAIGSRALASHDPAFTVHARWHRSLLGGLFNHVVQRLG
                     VPHIADTQCGFKLFRQSVAQDLFAVSCVDGYAFDLELLHIARQRHYRIAEVPINWTDQ
                     PGSKVRPWRDGSLMLRELLAIRKREAQGLYQPQSRPASSAVEPDLVTVEPTHS"
     gene            61544..62308
                     /locus_tag="PK589_03655"
     CDS             61544..62308
                     /locus_tag="PK589_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18719.1"
                     /translation="MRRSANCLASLVGMVIVTSCAVASPPSRLAQYVGSVVPEEAATA
                     TLPTSRPLHAALVVVADQSAPDSAPVLPDEALARVGALLTGEINRGYPVAVDRVVKLD
                     GMSPGGTAPSWPEVARQQGVQYLLVTVLSSTEQEYPISLFLGWTTHQQPGFRRDNWSL
                     AEAALIDGSTGRTVLQAEGRAMATLDRPTAPGISQWYPVIYLRPQDPERRIWPPTYEG
                     APLTLRLVSMEQAAKRLAGNLQRAWVEQRDLELAAR"
     gene            62480..64009
                     /locus_tag="PK589_03660"
     CDS             62480..64009
                     /locus_tag="PK589_03660"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNJ18720.1"
                     /translation="MVYRHERRRVRSVLAVTAMVAATFLSGGLPPRAGQAEAAGVPPA
                     FAQGFSEIVKAVTPAVVNIAVTGGGEGRREGRRQLPPGGPFGGPPPGPGGPGDEPPGM
                     EPPGGPPGGGPHRPEQSAGSGVILDPNGYIVTNNHVVEGATQITVTLSDRREFPAKII
                     GTDPKTDLAIIKIEAKDLSSMKWADYDELQVGDVVLAVGSPFGLSSTVTLGIISALGR
                     GNVGIADYEDFIQTDAAINPGNSGGALVNMQGKLIGINTAIFSRTGGSEGIGFAIPSS
                     IATDIVDSLTKTGKVVRGWMGVAIQEITPALAKSFKLPEQRKGVLISDVNENGPSHTA
                     GMRRGDVVISFNGKEVQSVSQLRNLVARMGVGKDADIKILREGKEQVLKVKVAERPSD
                     EVLAKREPAPSAAPAETVKPPDNVLAALRVQLLDAAMQSQLNIPAKTTGVVVSSVEAG
                     SAAESAGLQRGDVIQEVNHEVVKSLEDYQKASAKVKKDEMVVLLLSRQGNNLFVAVNP
                     K"
     gene            64101..65048
                     /gene="tatC"
                     /locus_tag="PK589_03665"
     CDS             64101..65048
                     /gene="tatC"
                     /locus_tag="PK589_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNJ18721.1"
                     /translation="MADGFKFQEWLQDTVFKPLEDKKMPVMEHLVELQVRLTRAVIVT
                     AIIFVGTFFYADTLVKWIRIPLQNMFVPGSLSWIPTDLPTVPFVFLAPAEALWQNVKV
                     AGLFALVLGTPYILVEFWHFVVPGLHAQERRFVGPFVILSTLAFYLGLLFSFFFVLPF
                     ALNFLISYGVNAGFIPQLSIAQYVGFALWFLLVFGLIFEVPLVITLLAKLGWVDAPLL
                     KRYRKWAFLTAFIFAAILTPTPDPFNQCLMALPMYIFYEVGIVSAGIFNKKKADAPAE
                     PLVPAVVAAGPGLAKAGPTIPSGGGDDEYVGVPSGGRRG"
     gene            65130..66086
                     /locus_tag="PK589_03670"
     CDS             65130..66086
                     /locus_tag="PK589_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNJ18722.1"
                     /translation="MPRELNVISQPGSSSGGDACTYMWACAICDENETCQKDKEGHSR
                     WLVAKRMERIEYKVLVMSNKGGVGKSTCTTNLAVSLALKGWHVGICDMDIHGPNIPKM
                     VGAEGQKLKISTSGGIIPFQAYNMKIASMSFLLQNSDDPIIWRDAYKYEFINQLLGGV
                     EWQDLNFLLIDLPPGTGNESVTTIDLLGGVSGAVIITTPQEVALLDSRKSVTFCKDSE
                     VPIIGIVENMSGLECPHCHKEVNVFRKGGGEASAADMGVPFLGRIPLDPDVVTQSDAG
                     EPFALFNSDAATAQAYHDIANQVEAFCKKSGSLIKPAPRQQH"
     gene            66101..67399
                     /locus_tag="PK589_03675"
     CDS             66101..67399
                     /locus_tag="PK589_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNJ18723.1"
                     /translation="MKSADATVGLTPLHEAQRIVLDAAAPLGLEKVSIMDTLGRVLGE
                     DIIATRSNPPWDNSAMDGFAVRAEDIAQEHAISKPVTLTVIEDVPAGKMPTKSVGKGQ
                     AIRIMTGAPIPAGADTVIKVEDTEHTPDSVRVFKAEPRGSNIRPQGEDVQKGDCIIPK
                     GTQIRSGEAGMLAILAKSFVLVYQRPRVAILSTGDELADLDERFSEEKIINSNSYGIA
                     AAVQEAGGVPILLGIARDTPAALTEKISHGLNADILVLSGGVSMGDYDFTKAVFRDLG
                     AEMNFWKLAIRPGQPLAFGKIQGKLAFGLPGNPVSSMVTFEQLVRPAMLKLSGARSYG
                     RPLLEAVFQEKFSKRTDRRHFLRGVLWREDGVFKVRTTGDQGSGILTSMVKANCLIDI
                     PVEVERLNPGDPVTVQLLSGSVWLDQAAPAASAAHRPSCC"
     gene            67421..67930
                     /gene="mobB"
                     /locus_tag="PK589_03680"
     CDS             67421..67930
                     /gene="mobB"
                     /locus_tag="PK589_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNJ18724.1"
                     /translation="MSVPILSFVGRSNSGKTTLIERVIPELVRAGYKVATVKHAGHGF
                     DLDTEGKDSWRHKQAGASSVVIISKSSLAMFADVSDHMNVEDVRERYLDPSYDLILAE
                     GWRSEGYPKIVVVRDQIGEVPVSQDGLLAVVSNKPVETSVPLLDPDDVAAVAALIIRH
                     FPKSQRDDA"
     gene            67920..68627
                     /locus_tag="PK589_03685"
     CDS             67920..68627
                     /locus_tag="PK589_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNJ18725.1"
                     /translation="MMRSTPAIVFGAILAGAVAFGGVAVPLTNHPKFCASCHTIQPAY
                     EGWLQSSHKEVECVACHVRPGVVGWLHDKAWHGTRDVAIYLFGKPTEPHNLQAQVDSA
                     VCLGCHRNILRVSEIAPRDLPSPVKDVGLIMSHRKHMAAFDQRAQGEGCTTCHAAVVH
                     ERPIKGYPIVIPRGHVSADSRPWNSTHPEGSVLHKRAQADCFRCHDNKTEYEGRVLSR
                     KCETCHLPEKLSEFLSF"
     gene            68647..69372
                     /locus_tag="PK589_03690"
     CDS             68647..69372
                     /locus_tag="PK589_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ18726.1"
                     /translation="MSSPVVEVRNLSKRFGGFTAVDGISFEIGRGEILGLLGPNGAGK
                     TTTFQMMLGLVTPTAGSIRMFGLDLQSNRETILQQVNFSSTYISLPFSLTVEENLRVV
                     ARLYGMSDIAARVDDMVKKLEMGDWRHKLTRKLSSGQMTRLTLAKALLTEPKVLFLDE
                     PTASLDPDIAHKIRAILLEERQATGLSILYTSHNMREMEEMSDRIIFLQRGKIVAEGT
                     AKDIVARFGQADLEDVFLKLARE"
     gene            69420..69896
                     /locus_tag="PK589_03695"
     CDS             69420..69896
                     /locus_tag="PK589_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18727.1"
                     /translation="MVDAGISFTVGVLIGGLSLMVGWGLLWFVVGGIGLARQTSGWPI
                     FISSISSTAIAGGVMAVVFWAVDAARLSSPALHVGLAGIPLALLAASAFRLEDGRRIG
                     PAFVQGSRLMMQELLGGHQEEGGCGHCHEQPVEEHPCHQKPCFEQSCHEKPCEGKL"
     gene            69903..70694
                     /locus_tag="PK589_03700"
     CDS             69903..70694
                     /locus_tag="PK589_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ18728.1"
                     /translation="MAYHRIAALVIRHLYLYRRSLPRVMEIIYWPFLDLVVWGFITVY
                     LATFQGQMPAVVTFLLGALILWDVLFRSQQGITISFLEEIWARNLMNLFASPLTPSEF
                     LAATMVMSLFKVTAVSVVMSLCAWVFYGYNVFIIGLWLMPFIANLVLTGWIIGVFTTS
                     LIMRFGQEAEVLAWSMVFLFQPISCVFYPMDVLPAWLRGVAAINPAAHVFEGMRGLLA
                     THAAPLTNLGMASGLNLLYLGAVVFWFHHTFNVCKERGSLVRVGE"
     gene            70735..71409
                     /locus_tag="PK589_03705"
     CDS             70735..71409
                     /locus_tag="PK589_03705"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HNJ18729.1"
                     /translation="MKVVAGQVGQARSVAGLENFSFHKTPVLVIENFWSADERGQFRE
                     AMARANWNQLQEMSYVRQDFPNSGNWAKAEIAQPQGRLLLSRLELPCIQQYIESFPNI
                     TRRHMGFSYYSYGAGDCLLTHDDTDQGHPTSGRPAPRRRIALVSYFHEEWDCDWGGEL
                     MIYAPGGESKAGKPDLAITHCIAPKPGSLVMFTVPRFHRVCRVDQTAGDHKRLSIAGW
                     FMTEHA"
     gene            complement(71565..>72118)
                     /locus_tag="PK589_03710"
     CDS             complement(71565..>72118)
                     /locus_tag="PK589_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ18730.1"
                     /translation="VNHYLHAGHIQPCAEWPDLSQAPGNEVVRLRAVRAQTESGLIKD
                     RFDIRRPIDLEVEFDVLKPGQVFVPVFNLYNEEDVTVFIAHDRDPAWQRTPRPAGRYT
                     STARIPGNLLAEGMLTVSPLMMTEDPFRLHAHAPRALGFRVDDSADGDSARGDFHGRW
                     PGVIRPLLEWRTRFEPVRDGRSG"
BASE COUNT        14153 a        19890 c        22683 g        15392 t
ORIGIN      
        1 cagccacgga gatggcagac ccatacccga tgcggccttc cgtaaataac gtttggtaac
       61 cataaaactg cagcacctgg gtggcgtcgc ccgggccgcc ttgcgtcatc acaaacacca
      121 gatcaaagac gcgaaacgca tccatggtgc gaaataggag cgcgagcaac agcgccggtt
      181 tgagaagcgg cagggtgatg tgccggaacc gctgccacac tgtggcgccg tcgacgcgtg
      241 cggcatcgta gagatcgtca ggaatgactt gcaggccggc cagaatcagg agcccggcga
      301 agcaggaggt tttccacacg tcggccagga cgacgacggc aaaggccacg cttggatagg
      361 ccagccaggg gatatagtcg gtgacgcggt cgccgaacag gagcagattg gcgaatccgt
      421 actggtcgtt gaagatgtag cgccagagtt gcgaggcaac gactgttgga atcgcccagg
      481 gaatcaggat cgcgccgcgt accaggcctc gtccacgaaa tcgttcatga atgacgaggg
      541 caatgatcag gccgcaggct aactcgagca gggtagaaag gactacaaac ccgagagtca
      601 cgacgaacgc ttgatgcgcg accgggtcat ggagcagcgc ctggtaattc tccaagccca
      661 cgaatggact gccaagttgg ggaatgccga cgaagatgtg atgcaggctg agccagagcg
      721 aatccgcgac gggatataac gcaaagacca ctgtgaccag gagtgccggt gccaccatca
      781 accaggcggc caggccctct cggcgtcgaa gtacggcatg cgcacgcatc atggcgccgc
      841 tttcgtcaat gccagaagtc gatcgatgtg cgcatcggtg acggcagctt cttgcgcgag
      901 gtcgatgtcc tccagcgcca gggcacggct gaagtagcgc tgcaagagat gtgagatggc
      961 gggatacacc ggcgaacgcg gacgaggcgt ggccgcgtgg aggacgctac gctgatttct
     1021 gagctgcggc gaatgggcca ggacctcagg gtcggagaag agcacttccc gggacggcgc
     1081 gatgccggct tcacgcgaga atttcttttg gacagcctgg ctggagagaa attcaatgag
     1141 agcccaggct tcgtccggat gtggcgaata ggcgctgatg ccgtacagcc agccaccgag
     1201 tgcggccgcc gtccgtcctg gcgagaatcc cggtagaggc gccacgccta cctgtccggc
     1261 aatggtcgac cgcgtccggt tattcgcgag ttcccaggca tagggccaat tccgatgaaa
     1321 gatcgcgtgt ccttgcagaa atagggacag cgcttccggt tcctgatagg tcagggcggc
     1381 acgggacgtg agccggccga tgactcgatc acggacgaat tgcacggcgg ccagtgcgcg
     1441 aggcgacgag agggtggacc cggttccatc cggtcgcaac agacttccgc catgcccctc
     1501 gacaaattcc agcatgttac agaccaagcc ttcgtactgc ttgaattgcg ccgagtagct
     1561 ttgaagcagc gggttgctcg ctcgttcgcc cgccaggatg gtttctgcct gctggatgag
     1621 ttccggccag gtcgtcggtg cggcaaatcc gtatttttcg agaaggtcct ggcgatagta
     1681 gagcagcccg gcatcgatgc ggctcgggac accgtagtaa tgggtgtcat accggccgac
     1741 ctcgacggcg gcaggcagaa agtcttgtcg catggagggg gaaaatttat cgtcgagcgg
     1801 gcgggcccac ccggcctctg caaactccgg gacccagatc acgtcgataa aaaagacatc
     1861 gacagtggca tcgcggttct tcaacttctg cgtcaggaga tcgtgatacg ccgtcgagct
     1921 gtgcggggcc agttcgcgca ccaccgagat ctggggatgt gcacgggtga agtctccgag
     1981 cacctcatcc cacacgcgtg catgatcggg tttccaggag acgaagcgaa gcgtgacgcg
     2041 gggcttggga gtggtggcgg tagtcccggg cagtgattcc gcgctcccga ggcttgtact
     2101 gccggtgagt acaatgagtg tgagcaggac gatccagcgg gataccgtga cggcacacga
     2161 ctgtgaagga cgctggatgc gctgacgtga agatatccat cgcatcactc tgtcaggtta
     2221 tacacagtcc ggtggggtga tgcaaaacca ccaggccgca gaagggtgcc tgtgccaact
     2281 gcggcagact gcacaatttg gggctctcaa gaagtgtcac gcctacaccg gcatggggcg
     2341 tcactgagat ggtcgagaaa aaccgttgtg aagacggagg cttgcgaagg tgctccttct
     2401 ggcatgacca ctgctattgt ttgtagaggg gaagtggcct tgccgacgtt tcgacagatc
     2461 gcagagagag cagttcaatc agaggcccgc cgattctcgc gggcgctggt gtgaaggagg
     2521 caatatgaat ggcctgcaag tatcagcggt ttcgcccagg cggggtcgcc tgctgcggat
     2581 gactcttggt gggttactgg gatgggctct catcctgatt gccatcacag atgcctgggc
     2641 tgttcaagcg agtccggtca gcctcagctt tagtgccgtg cagggcgggc cgaatccggc
     2701 gagccaggtc gtgaccatct ccaagagcac atcgcgtcag gtcggatgga aggccgttga
     2761 cgatgctgcg tggttgagta cggctccttc ctcaggaagc ctaaccagtt cagcgcaggt
     2821 cgtggtggcg gtcaatgtaa gtggattggc tgccggtacc tatagcggct cgatgaaggt
     2881 gacattgagt aaagggggaa gtttgtccat ccccgtcacc ctgacggtcg cgtcggcaac
     2941 gtccacaaaa tcgacgacga ccgtcacgac ggcaaccctt tcctgggcgc ccaatacgga
     3001 tagtgacctc gccgggtaca aggtgtatat gggcacgacc tccggtctgt acggtacgcc
     3061 ggtagacgtc gggaatgtga ccacctatac cgccggcaac ttaacaactg gaaccacgta
     3121 ttacttttcc gtgactgcgt atgaccaaag ccggaatgaa agcctccact caggcgaagt
     3181 gagcaagagc atctattgat ctcgtgctcc ttcccgagtt gccattaccg gctaagtaca
     3241 cgtcttattt cttgctctgc acggccaatt cccaccggta tccgtcgggg tccgagaacc
     3301 agatccccat gctcttggat cccggcgcca cgggcccgat ttcatctccc ggatcctcga
     3361 cctgaacctt cagctttttc agatgggcca gggctttttt gagtgcggcc atcgtgggta
     3421 gatggaatgc cacatggccg acggttttcg gcgatggttt tcctttggag aagacgatca
     3481 gcacattggc gtttccaatt cccacgccgc cttcatattc aaactgtttg ctgagccccg
     3541 ctacccgcat gaaccacttg gcgcttgcgc gcgggtcacg aaccgccagc ccgaaatgcc
     3601 atactgcgcc gagcgtaaat ggaaccttca tatccgtttc ctcctaacgg tgaaatgagg
     3661 cacatcctac catggcggcg gtggggcttg gcgctgcaaa acgcgcggag cccgccatcg
     3721 cctccttctc atggttgagc agccaccgtt tgcggttgag tccaccgccg tagccagtga
     3781 gggagccgtc ggcgccgatt acccggtggc agggaacgac gatgctgacg gggttcgcgc
     3841 cattggcgag cccgactgcg cgcaccgccg tcggttgccc gatgcgtctc gccaggtcac
     3901 cgtaactcac cgtttctcct gcgggaattt cgcgcagcgc gttccagacg cgtttctgaa
     3961 acggggtgcc tgccgtcgcg gtgaagagcg cgtcgatggc gtggagattg ccctcgaagt
     4021 acgccgacag ggccgccgca tggcacgtgg aattgacggc cggcgtcagg agcacgccct
     4081 gtaaaccata gtggcgcgtc aacagtttcc gcatgcgatc ctcgaaatca acccaatcga
     4141 ccgcccgcaa ctgcccctgt tcgtctgtca ttatcactaa ggttccaatc ggcgtgatca
     4201 aggtttctgt caaaaggtgt agttgttgag acatgataca tctccgtgca ggtggtgaca
     4261 gggctattga tcggcggtcc agaggtattg tgcggcgtag gcgcgccatg gtcgccaggc
     4321 ctccgtgcgc tccaacagat gagaggcact ggtctgttct cctatcaggc gcctggcgct
     4381 ccgtatcaga ccgaggtcgg tggccgggaa ggtgtccggt tcacggaagg cacgcagggc
     4441 gatatagtgt gccgtccacg ttccgattcc gggaatcgcc cggagttgcg cgagacgctc
     4501 ttcgactgat ccaccaggca tgaatagttg tggataagag attgccgttc gtgcaagagc
     4561 gctgatcgtt gctcgccgcg cggcaggcat gccgagccgg ctcaagtcgg cggtcgcgag
     4621 acagcgggcc gtcggaaacg tgcgcataag gcgcctgtcc actgcgtgtg gtaagggttc
     4681 tccccacagc ccgaccaatg ttccgcccag ccgccgtgcg gccgcgaccg taacctgttg
     4741 tcccagcacc gcgcggacag ccagttcaaa accgtcccaa cagccagggg cgcgcagtcc
     4801 cggtcgcgcg gcgatgaggg gcgccagggt cacatccttt gagagatggg ctgcgatcgc
     4861 ctcgatgtcg gctgccacgt cgaattgatg ccggatgcgc gcgaccatgg cctggagggc
     4921 gcggaccgat ggaaaatcta tcgtggccat caggctgtgc ttgtccggtc tgtgctcgat
     4981 ggtgatagtg cccgtcagcc cttcatggag cacgctacgg cggtaggcat tctcttcgac
     5041 aacctccagg ccttcgatgg cgcgcgcccg taagtatgcc aacatgctgt cccagtcgta
     5101 cggtggccga taaggaatct gcagcatgac aggtgcagtg gcggcgaggg ttacgctcgc
     5161 cgccgtggtg tcacggcgca attcgcccgg tgctcgatga tagagggagc ggaacgcatg
     5221 attgaaccgg cggacactgc cgaatcctcc cgcaagggcc acatccgaca tcgagagttt
     5281 cgtctcgtgg atcaactgct tcgcaaacag caggcgtctg gactgggcca cggctaccgg
     5341 cgcgacgccc aggtgttgat cgaagagccg gcgtagctgc cgtgctccca taccgacgcg
     5401 catcgcgagc gcctcgaccg tgccgtcctc atgatcgaga aatccctcgg caatcagcgc
     5461 cagcgctcgg gagacggtgt ttgacgtgcc gcgccacccg ggcagttcgg gaacaatttc
     5521 cgggcgacaa cgcaggcagg cgcggaagcc ggctgcttgt gccgccgcac tgccggaaaa
     5581 aaaccgacag tgttcccgtt ttggcgtgcg agccggacag atcggccggc aatagatccc
     5641 ggtcgagatg acgccgacga agaggcggcc gtcaaatcgg gcatcacggg tctgcagcgc
     5701 gcggtagctg atatcgggat cgagcagttc catgcccaca ctctgccata ttcaacggag
     5761 aaattccggc cgttttcgga cgtgaccatt cgattgcgga ggtcacaaca aaaatcgtgg
     5821 gaggcgcgtc gtcggttgga tggtcaggcg cgaggttgtg cggcggtttt ggcctttgcc
     5881 atcatcttgc agtaggagca ggtgccggcg gtagtgggct ggccgcaggt gtcgcagggc
     5941 gcgagcgtgc gttgatcttt ctcggccatg gattcggtcg cgggggctgg tgcggtttgt
     6001 ttgtccagaa acccccagta gaaacgctgc ttcgtaccgg gggattctgt ttccagccgg
     6061 ttgagcactt ccttgtagag aatcattttc gagcccttgg ccatggggca ttcttcgaca
     6121 atgtagtcga tccggttgac cacggcatag gcggcgattt cccgttcgga caaccggcag
     6181 aggggcttga cctttttcgc aaatccttcc accgatgccg gtagggtggg gctttgctta
     6241 tcgagatagt cgttctgcca gtgcaggaca ttccccagca gccgtgctgc ttcgtcgtcg
     6301 aggttatggc cggtggccat cacgtcatac tcctgctcca ctgccgcgcg attgaattga
     6361 tagcgcttga tcgttccgca ggttgagcag gtcggacggt gaaggatggt cgcgagttca
     6421 cgaatgccgg cccccgcttc ctgctcgacg acatgcacca gcagttttgc gccatgggag
     6481 gcggccaccg tgtcggcata gtgccgcact ttccggtgag attcttccga ataggccccg
     6541 atgccgagat tcacgtagag cgcgtcagcc tggtatccga gtgcgaggag aatatgccag
     6601 agggcgagac tgtccttgcc gccggaaacc gccacgagaa tccggtcttc aggtgtaaac
     6661 attttgaagg ccttaatggc acgcgccacc tgttcgtgga caaaggtcgt aaagcaggcc
     6721 ttgcagaagg cggcattgtg ccgcggcaat ccgattaccg cgcgtgtact ggtcgggcac
     6781 ttcgtgcaat tcatggtgtt ccaggtgttg gcagtcagtc gtcaggtttc agcaagcggg
     6841 cgaagccggt cgacgatggc tcgagaaatg ccggtcgatc acggatcgtt gagctatgtc
     6901 ccgccggaga tcaccggccg gatttcaatc tggtcggcgt cggcaagcat ttcatcttcc
     6961 gtcacgagtt catcgccccg gatgaccaaa tgcgcttcca tgaccagatt gagttcgcgc
     7021 agcagttcct tggtgcgctt gggcccctta atttcaactt gtctgtgagg gtgactcaac
     7081 tgaacgagca tgcgcgatga tacggctgat acgcgcggga ttgcaatcgg atcatgatcc
     7141 gtcggagtca ggcggagaag tgtggtcgcg cgaggccgcc agaagcccgc ttgcggctca
     7201 ttcctgacgc gctagaggag agcattactt aatgttctga atgatctggt agatcagcaa
     7261 tgcgaccacg ccaaggaaca acatcgcgga ggccaaggcc agacgtttat atttctggtt
     7321 gttgacgtcc actgcactga atgaggaatt tttccaatca agattttgtt cgttcaccat
     7381 gtcctcctac ctctaccgtc ccggtattct ttgcctcccc atcccgatcc tcgggatgca
     7441 tatatgcatt cactgagtac tgaagccgaa ggccgtcccg ttccacccga ggccctaggg
     7501 atagcaccag gtttgtatac gctcgcatgc ctgggaattg caaggaaagc tactagacaa
     7561 aattctggta acgaccgagt tttatctaat cgcccgagcg agcgtgctgg agacatcact
     7621 gactgtgccg tacaagaaat cgtccgctag ggggcgagtt cccgaaacca gtgatacaga
     7681 tcggaatgcg cgatggcctc ttcgacgatc tcatgcccca gcgagttcca gtggttgtcg
     7741 gttgcgaatt cgagtggacg gcggtgttgg gcaaaatgct cggcgaatcg gggttgcagg
     7801 tcgaggactt caaacccctg ttggcgggcg tgctccataa aatactgtcg catgatatcg
     7861 aaataggacc cggtggtgag ctgccacgtg gtggcgttgt agatatgcgg gcgcataccg
     7921 tcgacggcga agaggatccg gtgtggcggc agaccggatg cttcggccac ccgcgtcaag
     7981 aacgtgtcga tcgctcgttg ggcatcggca attcgcacgg ggtcggcttt ggacccggtg
     8041 tttccgacaa atgcgaggtg gttcgatggg cggccaaatg ggaggtgttg ccacttcagc
     8101 acgaagttga ggaggtccgt gttcagcgtg acatagcgca ccagggcgga cgcgcgaatg
     8161 atgcggcgcc acagggaggg gtgatagtcg attcgttgca gcgccagggt tccatgcgca
     8221 tcttccacaa aatacgagtg ccccggttca ttcttgtatt tcaacagact ctcatcgaaa
     8281 tcattgccga tgatgacgaa gaccattgcc tggggatgaa actgcttcgc cgcataatcc
     8341 gcataggcca ggtagttgct cagagctgag ctggatgtgc cgaaggaata gacgcgttca
     8401 gttgaggggg agcgggtcat cagtcgtccg gcgacgctct cccgaaacgg caccatcaac
     8461 gcttcgacat aactgtcgcc gatcagggcc aggagcggcg acggctggtc gagtagataa
     8521 tcctggtcgt tgacgaagcc gaggttgttg gtgcgggcga cattgacgat ggcgaaattc
     8581 cagtcggcag accagacaaa cgtccggttc ggctccagag aacggacggg atgctcgtga
     8641 tcgacgacct gggggcgcgc tccctcgttg accggcagga accgcagcac cagttccatg
     8701 gtgagtagtg ccagcagaat ccccaagccg atactgatga gcgggaaact ggcccgtctc
     8761 ataccggacg ggcaccaagc tggagaggaa gagatgaaaa ctcttcagcg ggccctgcgg
     8821 aacggggggc tagcggtatg cctgcgatga agcccaatta tagaacggca tccctgaggc
     8881 atttcgccgc ctcttccggt gccaccggat taatgtagaa accggtgccc cactcgaatc
     8941 ccgccacctg cgtaagtttt gggatgatct cgatgtgcca gtgatagaaa tctcccgtct
     9001 tttcatggag tggcgagctg tgcaggatga agttatacgc cggcctggcc aggacggcgt
     9061 ccatgcgccg gagggcttcc ccgagcatag gggccagaaa ttcgaattgg gtccgttggc
     9121 actcctcgaa gtagccggcg tggcgcttcg gaagaatcca catttcaaag ggaaatctcg
     9181 gggcataggg cgtgaggcac aggaattccg gattctccag gaccacgcgt gtgccttccg
     9241 aggattcctg ccgcatgata tcgcagtaga tgcagcgttc tttttgctgg aagtgctgtt
     9301 tgcagccatc gatctcttcg aggacgctgg tcggcacgac cggcaaggcg atcagttgag
     9361 aatggctgtg ttccagcgtg gcaccggcgg cggccccatg gttcttgaag atcaggatgt
     9421 atcggagccg gagatccttc ttcaggtcaa gaatccggtc gcgataggcc cagagcacat
     9481 cctccacccg tttcgcgggg aggtcgccca ggctttcttt atgagcgggg gtttcgatga
     9541 tgacttcgtg ggcgccgatt ccgttcatgc ggtcatacaa gccgaggcct tcccggccca
     9601 tgtctccttc gacctgcagt gccgggaatt tattgggtat gactcgcacg gtccagttcg
     9661 ggccgtccgg ttcagaggca tggggccggt acgccaggat ctctctgggc gtcagccgtt
     9721 cctgtcccgg gcaaaacgga cagagggcgg ctgagggaag cggcgccgac ggcatatgga
     9781 catcctgagg gcgtccgctc cgttcagtcg aaataatcac ccagcggccg acgatcgggt
     9841 ctcttctcaa ttcgggcatc tcaactcctg tggttcaaat caattgggcg tgatgctcat
     9901 tgcgtaaaac gggcatcgat ccgagtggta gcgtgatccg cgagccgcgc accgttccgt
     9961 tcagactcgc cacatcgtgg cttcaaagtc gtcgtccggc cgagtaaagg tggccgggct
    10021 gtgatgagga taccgcgcga tttccatgcc gtgttccgac acggtcagcg tcagccgcaa
    10081 ttctgcgccg acctcgatgt ccaggtcttt gaacggaatg cctaattcga gaatcttttg
    10141 ccggcagatt gtgctgtacg aacctctgtc gcgataggtg ccggattcgt ccgcccgtgc
    10201 gagttgaaaa tgatccgccg cgccggcctt caaggcgaag gacagtctgt gttgttggat
    10261 gcccgaaccg atgtacagat ctgccgagca ttgctcctgg cgggtgtctg atcgctcatc
    10321 gaaatcgagg cggaggtaca gctgctcgcg atcgaatccg aagaaaatgg cggtgaacag
    10381 cccctcggat ttccacatcg ctcccagcgg cggtgtggga ttgatggtgc ccgcgccgcg
    10441 ccattcgaaa aagttcgatg ccaagccgtc cagagtcggg ctgataaggg ccagtggcaa
    10501 ttgcacgagg tcgaggccct gcggtgtgcg cgcttcgacc aaaggctgat tcaagatctc
    10561 gggcggcgtc atcccggcgt aggtccagac gttgcgcagg tgcgtgcgaa acaggcggtc
    10621 gaactcctgt ttgtagtcgg tatcgaaatc atcgccatac caccagaacc aatcgctgcc
    10681 ctcggcggca tacaattcgt cccaggcggc gcgggcctta tcgggctcca gtgatggcgt
    10741 cagttcgacg aggcgtgccc gtgtgtgctg caggagatcc caaccgcgat tgtcttcctg
    10801 gtggccgatc cagattttga agtcctgatt gatccaggat ccggagtgca gctgctcgag
    10861 tcgctgggtc ggcggcgcgg ccttcatggc gtcactcatg gtgttcaagg tcacacgaat
    10921 gccattgccc aattggagcc catccgattc gaaggcgcga aagagcaggg agagaaaccg
    10981 ttcgccgccg tcgtggtaat gctcccaggg attttcgccg tcgaggatca ccgcgatggt
    11041 tccgttgtcc agccggatat cataggccag gctgcggatc cgccggagca cgtcgtccgc
    11101 ggcggcctcc ggagtcgttt tgtggtagac gaacccgaat gcgtcggaga tgtcccgatc
    11161 acggaacacc atggcgaggg ggtgtgtctc ggtcccgacc gcatagggtt ggtagagatg
    11221 atagtggcgg ttccaccctt ggccagccat ctgcagcgag cgatagagga tgccctcatc
    11281 cgtggccagc cagcggatgc cggcttgtgc cacgatcggt aacacctccg ggcaaaccga
    11341 tccttcggac ggccagagcc cagccggagc gcggccgaac gtgcgtgtat ggtactccac
    11401 ggcgcgcctg acttgggcct cagcatcggc cggtgcatga aaccgggctg gaagcggcag
    11461 gtcgggtctg gcgcgacgcg tgaactccga gtcgatgacc agtggaagga tcgggtgaaa
    11521 aaacggggta gtggtcagtt cgatctgtgc gcgatcctga agcgccttat acatgggcac
    11581 aatctgacgg atggccgttt gctgtaaggc caacacttcc tgtttgtcct cttcggtaaa
    11641 cgcacgattc ttcgcgcgca attctgccag gcgggggaat cgttgcagac tgccgtatcc
    11701 gaaccaggcg aggttgtgcc agacctgaag atcgaggagc tcctgagtgg aaaactggcg
    11761 ggccagccgt tccaggtcct gtccgtggac atcgatgccc cgtttgacca gcagctcctg
    11821 gtagcgagga aacggtcgga ccatcgtggc ccagttcgct gaaaaaaaat ggcggatgag
    11881 gaaggctttt tccgtcggcg tgaggtccgc cgccggacgt tgtgcatgtt ccaggaacag
    11941 gtcacggacg cgtcccgcag aaaattcttc gagttgcaac agcaaggacg gcgtgaagtt
    12001 gaaggtcgaa cgggcttctg gaaagtgttc gagcaggaag gccatgtcgt aataggcctt
    12061 cgtggcatgc aagcggaccc agggcatact ggcggacccc gtgagcgggt ccgtgtaata
    12121 cggctggtgc atatgccaga ggaaacagag ttgtatggtt ttcatcggga agtttcttgc
    12181 agactcacgg gcagtatgtc ataggggtat gagcgttgca atgaaacggc cggattgatg
    12241 gcacacgctg acttcttatg gcagtttggt tgtcttgtgc gtgataggag tggttgaagc
    12301 ggagaatgga cgacaatggc atctgagcag atggtctcga cagagatacg aggagggtcg
    12361 ctggtcacgc ccctctcgat catgcggtat tggggaccgg ttgggctata tgcgggcctc
    12421 atctttttcg ggtcctcgat ctcgacgccg cccgaagcga tttcatcgtt gatcaaagat
    12481 atttccgaca aggtgctgca tttcagcgaa tatgcgttgt tcggagccct gatctatcgt
    12541 gccgctcgcc acggatccgg tccatggatg gcgcaacatg cggtctgggc ggcagtggcc
    12601 ggttcagcgc tgtacggcgt gagtgatgag acgcatcagt tatttgttcc gtttcgtcaa
    12661 tcggatgtgt tggacgtgat tgcggacaca gtgggtggta ccgtgggggc atggggctgg
    12721 cgctggatcg aacagcgtac gtaccgcgat taagttccta gtacgatcat ttcatcatcc
    12781 aatcccttcc gtgcggatcc ctgcctggcg tgaccctggt gcctgatcac tctctgcgag
    12841 tcgtatcccg cgcgtcccgc ctcttcgata gccccacacc gcacgcagcg catcgcacga
    12901 atccagacat ggcctccggc atcgaccctg tcaatgtaca gatcggcgac gaggtgtcct
    12961 tgacagtgtg tacagaagag accggaggac ttcgtggtgg aagcagtggc gatccctgcg
    13021 cctgtctgtg tttggatgag gtgtggcgac atgggtcctc ctgctcgtga tgatggtggg
    13081 tggtatacct ctatcggtac gcaaagaaga tgcctggagc acgcggggtg gagtgacact
    13141 gagggatatt gttgggggga aattatcctg gtgagtaacg gtgctgctga cgcggatgac
    13201 gagcgtcgtg gtgacgcatt gaagcgactt ggcgttgttc gagcggtcgg ggggagagtg
    13261 gctggtcgga acaatacgcg gcaatttttt ccgctgttgc tgcctcaatc aatacttacc
    13321 aggcgtaggc ttccggagct gcgcctccgg ggccgggaaa cattgcatct aacgtcttga
    13381 ggtgttctgt cgtcagcgtc agctcaagac tggaaatcgc ggcagtcaat tgctccatcg
    13441 tgcgggggcc gataatgggg gccgtcaccg ctggttgatg cagcagccag gcgagggcga
    13501 cggtggccgg cgcggtattg agcgtccggc aaaactgctc gtacgcttct aacttctgac
    13561 gatgggtctc gatatctttg cgaacgtcct cctcagcccg ccgaccgctc gtgggtgagc
    13621 tgagggcgcc gcccagcaat ccccgtgcga gaggactcca gggaatgatg ccgatgccgt
    13681 aggcctggca ggcgggaatc acctcgagct cgaccgtgcg ctcgagcaaa ttgtacaggc
    13741 tctgctccga aatgagtccc aggaagtggc gtgactttgc cagctcttgg gcctgagcga
    13801 tgtgccagcc ggcaaaattg ctgctgccga tgtagagcac cttgccttcg cggcagagtt
    13861 gctccatcgc ttgccagatt tcctcccagg ggcagtcacg atcaatatgg tgcatctggt
    13921 aaaggtcgat gtagtcggtg ttcaggcgcc gcagactgct ttcacacgct tgtttgatat
    13981 gccgcgccga gaggcgtgac tcgttgggcc agtcgcccat ccgtccgtac accttggtgg
    14041 ccagcacgac cttctcgcgc cgaccgcctc cctgagccag ccatcggccg acgatctgtt
    14101 ccgtgacccc ttctcccacc ttccagccat acacattggc gctgtcgaag aaattgatgc
    14161 ccaactcgag cgcgcgatcc atgatctgac cactgtcggc ttcggtggtg tgcgggccga
    14221 agttcatggt gccgaggcag aggcggctga cgcgcaaacc ggagcggccg aggtgaacgt
    14281 attgcatggg gcctccttgg ctagagcaat cagaataccg gcttccgatg gatactgaca
    14341 accaagctcc gcacgcgtgg atagctgcgg tgcgacggtt ggatgcagca ggtgagtttc
    14401 tgctggcggt tcggaacggt ttggctataa tgccgatcgt atgagcacaa caaatccagc
    14461 cgcgcccgcg gcaccgctta ctcctcctga tcctgaacgt attgcccaga cggtcgccac
    14521 tcgattgcac tttcgcggag agatgaccgc actcagcgcg ctggccggtg acgcatccaa
    14581 tcgccggtat ttccggatct ttctgaaggg cacgccctcc gggctgattc tcatgcagct
    14641 cgccgacccg gaagggttca agaaatccga agaagccgta agcggcgcgt ctgctcaaat
    14701 taccgagttg ccgttcatca acgtgctgac ccacttgcaa cgtaccggcg tgaccgttcc
    14761 gaccttgcac tactttgatc gcgaagcggg tctgttgtat ctggaagatt ttggagacct
    14821 gaccctcgcg gaagcctgcc ggggtgcaga tcccgcgagg ctcgaggcct tgtatcgcca
    14881 ggcgattgat caactggttt tgctgcaagt caaaggcacg acaccgcctg cgccgggatg
    14941 tattgcgttt caccggcgct tcgatgtgcc gctgttgatg tgggaattcg accactttct
    15001 cgaatatggc attacggcgc gtctagggca gcccatgaaa ccggaagatg attccgccgt
    15061 gcgcgcggcg tttcagcgca tcgccgaatt attggcgggg caaccgcagg tattcgttca
    15121 tcgcgactat cattcgcgta atctcatggt cgatggatcg cgcatgggca tcatcgattt
    15181 tcaggatgcc cttatgggac cggccacgta cgatctggcg tcgctgttga aggatgccta
    15241 tatcgaattg gatgacgcag tcgtcgatag gctagtcgag cattttctcg atggccttgc
    15301 cggcctgggc gtgaaggtca atgatcgttc agcgtttcga cgtgtgttcg atctcaccag
    15361 tattcagcgg aatctcaaag ccgccggccg gtttgtatat atcgatcggg tgaaacacaa
    15421 tccgaaattt cttgccgata tccctcgtgt tctggggtat gtcaaacgca acctcgcaaa
    15481 atatcccgag ctggctccat tgcggcagca tctcgcgccg tacgtgacag aactgcaata
    15541 ggcgctactc agcacctcct atgaaggcga tgatccttgc agccggcctt ggcactcggc
    15601 tgagaccgct gaccgatacc accccaaaac cgctccttcc ggtcgccggc acgccgatga
    15661 tcgtctggaa cctgctgctc ctgaagcggc acggcattca tgatgtcgtc atcaacctcc
    15721 atcatctcgg ctcgatgatt tcacaagccc tgggcgacgg tcgcgcgctg gggatgcgaa
    15781 tcgtctattc gcaggagccg gtcattctcg gcaccggcgg gggaatcaag caggcggagc
    15841 cgcatttcaa tggtgaaccg gtgttgatcc tgaacggcga caccctattc gaattggacc
    15901 tcggcgcgct catgaggttt caccgcgagc agcaggcggc ggccacgctg gtgttgcgca
    15961 aggatcccga ggcggctcgt tgggggctgg tggaagtgac ggatcgtagc gaggtggtgc
    16021 ggattaccgg ccgcggccgc gcagactcgg cgcagaccct ggcgcgcatg tttgccggca
    16081 ttcacattct gcatccgcgt ctgttacggt ccctgcctgc gggcaaggaa tgttccatca
    16141 tcgatgccta tgtgcagggt atccaggatg gagagcggat cgtaggctac gatttcgacg
    16201 gattttggag cgatgtgggg acgccggagc ggtacgcgca agtggagcga gacgccgctg
    16261 ccgggcgact caatctgtct gctcgctcag cgagtaacta agctgaatat cgggggaatg
    16321 ccgagtcgaa ggtctggact ctttgagcgc gctagcgaat ggagttggct gtacaggatg
    16381 gtcaaaacga ttgtccagca aggccgcagc gagtgaaggg ccgaggcgta cccgtagggt
    16441 acgttgaggg cctgagcgat gcgagaacga agctggcggt cgttttcacc atcctgcctg
    16501 gttactcggc ggcggcgggt ttccgctgct tgatcagtct cgcaagatag gttcgctgca
    16561 ggtccagaaa ctccgctgcc ttcgtctgat tccccgccgc tttttccaat gcccgatcga
    16621 tgatataggc actgtgcgct tccatggact catggtaggt gagattgaga tagggaagcg
    16681 cgtggtctgc gccgcttttg cctgcggcgt cttcgggggt cagggccaga aattccggtt
    16741 cgatcacgtc actggggctg agcacgactg cgcgtgaaag gacgttgtcg agttcgcgga
    16801 tgttgccggg ccaggagtag tgagtcagcg cagcccttgc cgcggggctg agggtgacgc
    16861 ccgggcgctt cgcttcatag gcatgccgct tcaggaaaaa ttccgccagt tggacgatgt
    16921 cttcgcttcg ttcccgcaag ggagggagtg taaagctgac gacattgagc cggaagaaca
    16981 aatcctcgcg gaactggccc gccttcaccg attgtcggag atccttattg gtggccgcga
    17041 tgaaacgaat gttgacggac accgagcggg taccgccgac gcgttgaaac tctttgtctt
    17101 gcaggacgcg cagtagcttg gcctgcaacg gcaggggcat atctccgatc tcgtcgagaa
    17161 aaaccgttcc gccctctgcc atttcgagtt tgcccttctg aagccggtcc gcgccggtga
    17221 aggcgccgcg ttcgtggccg aagagttcgt tctcgagcag cgtttcggtg agggccacgc
    17281 agttgatgac gaccatcggc atgctctgac gggggctcca ttgatgaatg gagcgcgcga
    17341 acagttcctt gccggtcccg ctttcgccca gcaggagaat gctggcgtca gacttggccg
    17401 cacgctgggc cgactcgatg atgcccctga ttttttggct ttccccgacg atggtggcat
    17461 agcggttttg gacgtcggac ttgagggcgg cgacctgtcg ctttaacgag tcccgttcca
    17521 gcgctttttg aatcacgatc agcaggtgat ctttttccaa cggcttggtc aggaagtcat
    17581 acgcgccatg gcgcatggct tccacggcat ccggaatggt gccggcggcc gtcatgacga
    17641 tgaccgggat ttcttcgcat tgcttgttct gggccatgcg tttcaggacg tccatgcctt
    17701 tgaggcgagg caggtacaga tccagcagga ccaggttggg cgattcctgc tcgatgagtt
    17761 caagcccacg ctggccgtcg cccgcggtga gcgttttgta tccgcccgca tcaaggcgat
    17821 cctgtaacat cgtcacgatg tcttgatcgt catcgactat gaggactttg gccttcatcg
    17881 aaccctgtgt ccggcgccta gccttccatc acattcatga ccaagccggt gagcggcttg
    17941 ggaaagaagt aggtcgactt gtggggcatc cgttcgcccg ctgaggcgac cgctttgact
    18001 tcggccactt tcgtcgggtt gagcaggagg gcggccgtgc cttttccctg gcgcacccag
    18061 ttcagcgctt cgtgatcatc tttggaatac aacatggctt cctgctcctg ctggctcggg
    18121 cagagcgtgg caatcacatg ctgctgtagc agcgacacgt ccagccgatc gcgcggtgac
    18181 gcggaggccg tagggcgatg ggattcccgc agggtcagca ggtaataatt cgaatcgttc
    18241 ttcagagcca aaccgaacat cggcacggac tgtccctggc tgcgtagccg ttcgatgaac
    18301 tgcgcgcgca cctgcgattc attgcctgct tgaaggggga gcgcagcgac ctcaaacacc
    18361 ggctcgaatt tccccagcag atccttcgtc gccggaaccg gtgtcgtcag gacccgatgg
    18421 gtcggcagca cggtcaggcc cttgtcctcc aagctggcga acagcatcag cacattgtcg
    18481 tacggttgcg gtgaggtggc ggctccagcc tgttgccgtc tggatttccg gtagttgaga
    18541 gccgtttcgt accgatgatg gccgtcggcg atgaacagtt gtttcgtgtt catgatctgg
    18601 acgacctttg cgaggacctt cgcatcggtg acgctccaca actgctgccg gaagccctcg
    18661 tcgtcctgga aatcgatgcg cggtttctcc gacgcaatcg actgttcgag caaattgagc
    18721 acttcattct ggggatccga atacagcgaa atgatcgcgc tgaagttggc ccggcaggct
    18781 tccaggagat tgagccggtc ggttttcgct gctgcgcggg tgttttcgtg cggatagatc
    18841 ttgcccgaac cgaattcttc gagctccact gtagacagga accctttgaa gaccttggtc
    18901 ggtgtgccag gtgccgagta gggcggccgg tattcgatgg tgtggtagta aaccgccggc
    18961 tgggaatctt tgcggagggc tccgctcttc agccagtcgg tcagggcagc cgcggcgcgg
    19021 gtgtacttat tattggcagg tgcgtcccct gcttgttcgt agccgagttc cagccggatg
    19081 acgttgttcg ggtggcgatc gtgcaacgtt ttttgcagcg tgctgtcgat gatgtcgtag
    19141 ggcggtgcga ccacttttcg gacgtcgccg accgtcgtgg gattatacag ggtgccgtga
    19201 aagggaatga tatgtgccat gcgattctat cctttttgct cgatggatgc agggtggcat
    19261 acgggttgtt gggaaaggct gccgattgcc ggactcattg acggcctttc cgaataacct
    19321 gcggcggtcg ctctgtcact gaatgcaacg tgaagcgtct tatgaccgcg gtggtagtgg
    19381 taggggctta acatggttat cggttcatcc cgtggcgaaa gagacccgtc gctgaacgag
    19441 tacgtgcggt cagcagagcg tgggcgttgt agaagccgga cggcggcaac tcagcggtcg
    19501 cgagtcacca tataatcggc ggcgatggac agggcgcgct tggcggtgtt gtcgtggaac
    19561 agatcgagat cgcgtttcgc ggcggcgaca aacgcgtgcg cccgttccat ggcgtaggcg
    19621 atggacccat actcctgcat caaggcgacg atgcggcgca gttcctcatc ggtcaaagtc
    19681 cgggtttcca tgcgatcttt gatcaactgc cggtctggtt ccgggcagtg ttgcaacaga
    19741 tgcaacagcg gcagcgtcgc tttgccttgc cgaaggtcct gccccaatgt tttgcccaga
    19801 tgttcgccgt tggcagtgta gtccagcgtg tcatccgcca gttggaatgc aatgcccagc
    19861 cgctgtccga agcggaacag cgcctcctgt aattcttccg tggccccgcc gacaatcgcg
    19921 ccgatcttgc acgaggcagc aatcaatccg gcggtcttat gctcaatgat gcggaggtat
    19981 tccggttccg gcatcagcgg attgccgttg taatacagtt ggagcacttc gccttcggcc
    20041 atcttccggc aggcttccga gagtgcttcg ttgatgcctt ggttacggaa gtcgacgatc
    20101 tggcagatgg cccgtgaata gaggtaatcc cccaccagaa tactgatctg gttgccccag
    20161 accttgctcg cggtacggcg gccccggcgc aggtcggcgt cgtccacgac gtcgtcgtgc
    20221 agcagggtgg cggtatgaat gaattcgaca aggcttccca gggcctgatg gtcctggccg
    20281 gcgtatccgc agagatgggc gctgaggagc acgaacagcg gtcggacgcg cttgccgccg
    20341 ctgttcaaga tgtgggcggc gactgtattg acgagatcga cgctggagtc gagatttttc
    20401 cggatctgtt cctcgacccc ctcgagctct tcacggtaca cgtcccagac atcggccatg
    20461 ctgtggagcg tgagggtggt cggtccattc gacatggggc ggatagtagg gcaggaaact
    20521 ccactaagtc aagcactggt cggagggttt gtacttcagt ttgcgtcgcc gttctcttga
    20581 cagtttcacg gcccatccag taaggtgacc tctcgctgat ttccccaact gaatggtagc
    20641 agtcaccgtg gtcgtgcgac agcttcaacg tccccgagag gggcgtctgt tccccggttt
    20701 attgcctgcc tctagacgag aatacgtgcc atgaatgtac cagggcttcc tcccaaacaa
    20761 gggctatatg atccgcagca cgagaaagac tcgtgcggag tcgggtttgt cgtcgatatc
    20821 aagggccagc gctcgcacca gatcgtgcag caggggcttc aggttttaga aagcctgacc
    20881 catcgcggtg cgcaggggtg tgatccctgt accggagacg gcgcaggcat cctgcttcaa
    20941 gtcccgcatg agttcttcaa gcgtgccgcc aaagacagcg gcatcaagct gcccggagcg
    21001 ggagagtatg gcgtcggaat ggtgttctta ccgcccgatg ccgatgcccg cgcgcagtgc
    21061 gagacggtgt ttacccgggt cattaaagat gccggtgcga agctcctcgg ctggcgtgat
    21121 gtgccggtca agagcgacgc cattggaccg gtggcgcgca gcactgagcc gttcatgcga
    21181 caggtcttca ttgcccgtgg catcttcacg gatgaggaat tcgaacggcg cctgtatgtc
    21241 atccgcaagt gcgcggaacg ggccgtgcgc gaatccgcca ttgaaggccg ggactatttc
    21301 tacatcccga gtctctccag cagcaccatc gtgttcaagg ggctcctgct cccgcatcag
    21361 atcccgcagt attaccagga tctgaccgac agcagcctga cgagcgccat ggcgctggtg
    21421 cattctcgct tcagcacgaa tacgtttccc acctggccgc tggcccatcc ttatcgctac
    21481 atctgccaca acggtgagat caatacgttg aagggcaatg tgaattggat gcgcgcgcgg
    21541 caggggcggc tgaactccga gttgttcggc gaagacatga agaagctctt cccgatcgtc
    21601 tatgagaatc agagcgactc ggccagcttg gacaatgcgc tggaattcct ggtcttgggc
    21661 gggcggtcat tgccgcacgc catgatgatg ctgatccccg agccctgggt ggccaatccg
    21721 cagatggatt tggatcgccg tggattctat gagtaccacg ccgccatgca ggaaccctgg
    21781 gatggtcccg ccgcggtgtg tttcaccgac ggcaagttga tcggggccac gctggaccgg
    21841 aacgggcttc gcccctgccg gtatcaggtg acgaccgatg gattggttgt cctggcttcc
    21901 gaagccggtg tcctgccgat ggatccgcag cgcattcgtc aaaaaggccg gttgatgccg
    21961 ggccgtatgt tcctggtcga taccgaacag ggccgtatca tcgacgacga ggaagtcaaa
    22021 gccgatatcg ttcgtcgtaa gccgtaccgg tcctgggtca ccgagcatcg catttccctg
    22081 gatgagttgc ccgatccgat caacgtcccg cagcccgatc atcccacgat ccggcagcgt
    22141 cagcaggcgt tcggctatac catcgaagag ctgaagatgg tcatcacccc catggtggtg
    22201 gaagggcagg aagccatctc ctcgatgggc accgatacgc cgcttgcggt gttgtctgaa
    22261 cggccgcagc tgctctttaa atacttcaag caactctttg cgcaggtcac gaatccgccg
    22321 atcgatccga tccgcgaaga actcgtgatg tcgctcacca ccagcatcgg ccccaagccg
    22381 aatctgatgg atgagcatcc ggaatcggcc cgccgcatcc gggtgaagca gccgattttg
    22441 accaatgccg atctgcacaa gatccgggag atcgccgatc cgcacttcaa gagcaagacg
    22501 ttgaagatgt tgttccgcgt cgccgaaggt ccggaaggat tgggcgcggc ggtggacgat
    22561 ctctgtcggc aggcgtcgca ggcgattcgt gaggggtaca agttcctgat cctcagcgac
    22621 cgcggcgtga atgccgaatg ggcgccgatc ccgagtcttc tcggtgttgc ggcggtccat
    22681 caccatctgg tgcgggaatg cacgagaacg gaagtgggcc tgacggtgga aaccggagag
    22741 ccgcgcgacg tgcatcattt cgcctgcttg atcggcttcg gagccggaac cgtgaatccc
    22801 tatctggtct tcgaatcgct ggtggatctg gaacgcgacg gatactttcc ggaagggctg
    22861 gacgcggcga ccgccgaagg caagttcatc aaagccatta acaaaggctt gctcaagatt
    22921 ttctcgaaga tgggtatctc cacggtgcag tcctactgcg gcgcgcagat cttcgaggcg
    22981 atcggcctca atcatgaact gatcgaccgc tacttcaccg gcacgccgtc gcggatcgag
    23041 ggtatcggca ttcgggagat cggggaagaa acgttacgcc gccatcgcgt ggcctacgaa
    23101 ccggtgccga tccgacagtt agatttcggt ggcgagattc actatcggat ccagggcgaa
    23161 caccacaact ggaatccgga taccatctac aagttacagc acgcgacgag aaacaacgat
    23221 cccaagacgt tcgccgagtt ttcgcagctg gtcaatgacg agagcaaacg ccgctcgaac
    23281 ctgcgcggcc tgctggagtt caagttcctg ccggaaccga ttgcgctgaa cgaagtagag
    23341 ccggccaaag acatcgtcaa acgcttcacc accggcgcca tgtcgttcgg ctccatcagc
    23401 aaggaagcgc acgagacgct ggccatcgcc atgaaccggc tgggcgccaa gagcaatacg
    23461 ggtgaaggag gcgaggatcc agagcgattc gagccgctcc cgaacggcga ctcccgcaac
    23521 agctatatca agcaggtggc atccgcgcgg ttcggggtca cgagccatta tctggtgaat
    23581 gcaaaagaac tccagatcaa gatggcgcag ggcgcgaaac cgggcgaagg tggtcagctt
    23641 cccggacata aggtggacga gaacatcgcg cggttgcgct actccacgcc gggcgtgcag
    23701 ctcatttcac caccaccgca ccacgacatc tattccatcg aagacttagc acagctcatc
    23761 ttcgacttga agaatgccaa tgccgatgcg gccgtctcgg tgaaactggt ctccgaagtc
    23821 ggcgtcggca cggtagccgc cggagtggcc aaggcccatg ccgacaaggt gctcatcagc
    23881 ggcgactcag gtgggacggg agcctctcca ctgtcatcga ttaagtacgc cggtgtgccg
    23941 tgggagttgg ggttggcgga aacgcatcaa acgctggtgc tcaacgattt gcgcggccgg
    24001 attcgtgtgg agacagacgg ccagatgaag accgggcgcg atgtggccat tgcggccttg
    24061 ttgggggcgg aagagtacgg gtttgccacg gcgccgctca tcatcgaagg ctgcatcatg
    24121 atgcggaagt gccacctcaa tacctgtccc gtcggcattg cgactcagga tccggttctc
    24181 cgcaagaagt ttaccgggca gccggagcat gtcgtgaatt tcttcttctt catcgccgaa
    24241 gaattgcggc agatcatggc gaagctggga ttccgcacca tcaatgaaat ggtcgggcgc
    24301 gtcgacaagc tcaagattca caaggccgtc gaacattgga aggccaaggg gctggatctg
    24361 gcgccgctgc tcaagatgcc cgaggtcggc cctgaagtcc cacggtattg cgtgcagaag
    24421 caggatcacg gcatcgccga gattttggac cgcgcgctca tcgaacaatg tgcccccgca
    24481 attgatcgtg gccaaaaggt cacgctcgaa ctgccgatcc ggaacttgaa ccggacggtc
    24541 ggcacgatgt tgtcgagcca ggtgtcgaag aagtatggtc aggatggatt gcctgccgac
    24601 acgatcacga tcaagttcaa cggatcggcc ggccagtcgt tcggcgcgtt tctctcgcgt
    24661 ggcatcacgc tgatcctcga aggggagtcc aacgactata tcggcaaggg cctgtcgggc
    24721 ggcaagatca tcgtcttccc gccgaagaac gcgatttaca caccggaaga aaccattctc
    24781 gtcggcaaca cgtcgttgta tggtggcacg cagggcgaag cctattttta cggcatggcc
    24841 ggtgaacggt ttgccgtgcg caacagcggc gtgcgcgccg tcgtcgaggg aaccggcgat
    24901 cacggctgtg aatatatgac cggcggagtc gtggcggtcc tgggcaggac cggtagaaac
    24961 ttcgcggcgg gtatgtcagg cggggtggcg tttgtgttga acgagctgga taagttccag
    25021 tctcgttgca acttaggcat ggtggagttg gagccggttg tcagcgatga ggacaagcgg
    25081 ctgttgcacg atatgatcac gtcgcacttc ctctataccg gcagccggaa tgccaagcgg
    25141 attctggatg gctgggaggc gatcctgccc aagttcgtga aggtcatgcc gatcgattac
    25201 aagcgtgtgc tcgctgagcg gaaggccgct gcggccaaag tgcaaaaata gatagcgcgc
    25261 gtctggtgac gcgcacgtgt gagcaatcca gtcgatagac gagtgacgaa ggacgagaga
    25321 cgaattagcg atgggtgatc caaagggctt tctgaaatac gcgcgtgagg ggccgaagcg
    25381 gaaaccgatc gagttgcgcg tgctcgattg gaaagaaatg tacgagccca tcccggagga
    25441 gaagctcaag gttcaaggtg cccgctgcat ggattgtggt gtgccttttt gccagggcaa
    25501 taccggttgt cccgtcgtga atttgattcc cgagtggaac gatctcgtct atcgcggacg
    25561 ctggaaagat gcgctgaagg cgttgcacac cacgaacaat ttcccggaat tcaccggccg
    25621 tctctgtccc gcgccctgcg aaggcgcctg cgtgctgggc atcaatagcg acccggtgtc
    25681 gatccgcgtg ctcgaatgga acattatcga tcgtggcttc aacgaaggcc tggtcgagcc
    25741 ggccttgccg gtgagaaaga ccgggaagac ggtcgccatc atcggatcag gccctgccgg
    25801 tctggctgcc gcgcaacaac tcgcgcgcgc cggtcatagc gtcaccgtat ttgagaaggc
    25861 cgatcgtatc ggcggcttgc tgcggtacgg catccctgat ttcaagatgg agaagtgggt
    25921 catcgaccgt cggctggagc agatgaaagc cgaaggggtg gagttcaaaa ccggcgtcac
    25981 tgtgggcaaa gatgttaccg gcgaacagct acggcaggag ttcgatgcgg tggggctgac
    26041 gatgggcgcg gaaatggcgc gagatctgcc ggtgccgggg cgtgaactca agggcatcca
    26101 tttcgccatg gaatatctca cgcagcagaa caagcggacg gcagggatct ccgtatccga
    26161 agaaccgatc accgccaagg gaaagcgggt ggtcatcatc ggcggcggcg ataccgggtc
    26221 cgactgtttg ggaacggccc atcggcaggg ctgcagcgaa gcgcatcagt tcgaagtatt
    26281 gcccgagcca cctccgtccc gatcgagctc gacgccctgg ccgctctggc cgatgcagct
    26341 tcgtacgtcc catgctcacg aagaaggctg tgatcgtcaa tggagcgtgt ctaccacgaa
    26401 gttcaccggt cacaacggac atgtgaccaa actgcatgcc aaccgggtga agttcgaggg
    26461 cggtaagttc gtggcgattc cgaatacgga cttcgagttg gatgcggatc tcgtgctcct
    26521 cgccatgggc ttcacggggc cggtcagaaa cggttttctc gacagccttg gcgtgaatta
    26581 tgatgcgcgt ggctgcgtga ccgtcaacga gaacttcatg accaacctcg acggcgtctt
    26641 tgccggcggc gataccaagc gcggtgcctc cctcatcgtc tgggccatcg ccgaagggcg
    26701 caagatggcg gcggggatca atcagtatct gcaagcgaac aagtctgcca agtcaggccg
    26761 ctgaaaaagc ctcccggcgt cgttctcgct tccctcaaac cctcaacgta ccgaaacagt
    26821 acgcctcgcg ggctttcgct cgctgcggcc tagctgtata ggcttttgga gcggccccgg
    26881 gccgattcta atgtccttgg gcggtgcccc gctccagtct gtcttatttt tgccccgcct
    26941 tttaggatcg ctggtatttg tttgtctatc cccttgaatg gtgctgcgcc ggctttcgaa
    27001 cctatgtttc atgtcttggg ctacaatcga ttatgaatct gcctaatgtt accgtgcgcg
    27061 attcgcgcct ccgtacagtg ccggggctaa catgacgaaa ttcgctcgtg ttccagacgc
    27121 agactgggta ctagctggaa atgtcggaag tgcaagaatc agccgcctca gacaagccga
    27181 aaatttcgat gcggtcgtgt caaggatcat acgtggcgcc cgggtcgaac gcttgaggcg
    27241 agagcgttcg catgaagatc attcggcttt ccgcttgatc gtggatcttc gcgtgaaggc
    27301 ccaaagtctt gaccagtttt tcaattctcc tgttggctac cgcgcgcagt acttcgcgaa
    27361 ctgcaaactt ggcgagagag caaatcggtt cgtcatcaac gagttgtttt cgaaggttct
    27421 tgtggcagga aagagaggga gacgcctaaa ggaagatttt attcgactgt cccttaagca
    27481 ttcctctttc aaagttttgc tgcatcaagg ccagtggctt cggcatgcga aacgcgaaga
    27541 tcgagtcctt cttgttccca ggtggcaggc cgagtgcgtc tcgggggaca aattacgacg
    27601 gaaacttgct cgatggggat cgctggctcc agcgcacgaa acgcgacttc tgctgaaggg
    27661 aggcttcgta agggcgacca gcagagtaac tatcgataag tctccaatga ctcgatctag
    27721 agagcttcac gagcttggat ttacctagag aggcaggagg tatgagtgag aaggaaagct
    27781 agaattgact gaagcagtgc aatcaaaggg tcgcagctga acagagtcca gaacgccttc
    27841 catcgatcgt gaaaattgga agcagcaacg ttttgccagc gctcatcggc cctcctagac
    27901 ttctctcgtc ctcgcctgta tcttctcggg aattacattc aggagaattt acaatgactc
    27961 agcaaaccta tccccgtagc cccaaagtcc tcctcggcgg catcgcccac ctgggccggt
    28021 tcatcgataa agtccgcttg cgccacgccg gcaagattca ggactacaac tacatcaccg
    28081 tcgggttcga taagtatctg atcgattttt tacagatcga tgcgaaggcc ttcgagcaac
    28141 aggtgttggc gggcgagagc gatgagaagt tgttgagttg ggtgaagacc cacggccgga
    28201 aacattccga ggaagaaatc gcgcaatggt cgaaaggtct tctggcgggc ggcccgaagg
    28261 acgatgcggc gaagcagcga ttccagggac gggtgcagga catcgccacc aagcgaggcg
    28321 ttccgctgac ctcattgccg cctgtgacga cgtgggctga tgtaatcgag ctggacgaag
    28381 aacggatgta gccttacgac agtgaatcgt gggcgatgac tatcgtactt cggtccagcc
    28441 cgtggattgc gacttgaaga tcacggtgcg tgatgaggtg cggacgacca tgccgttctg
    28501 actgccatcg accgacagca cccgttcatt cggggtccag tcaatgttga agaagcctcc
    28561 cgtataggtc gaaaatccca gtgcccgctc attggtgatg gcgacggcga cgtgcccttg
    28621 gccgcgaagc tgtgagacgg tctcattcgg tcccagcgca atggttgtcc agtgcccgcg
    28681 cgtttcctgg aacccaaata cttgctggtt gctgtgcgcc agaatcagaa acggcagtag
    28741 ttgatgccgt ccgatccgtt cttctactcc aatcgacact tcccgccatt gccgcaggcc
    28801 ggcagaaaac cctaacaacc tgctcgaggt ctggacgaat ccggtgtgtc cgcgtgctgc
    28861 ggaactgatg accgtttcat tcggctgtaa ggattcttca acgccgcccc ctgcagctga
    28921 ccaggcaatg actttgctgt cggttggtgt gatggtgacc tggctgcctc cgccttgagc
    28981 gtgcgaaggc tcggcggtca cgagtagact tgcggttgaa gtggttatga cgaacagcaa
    29041 tagggcgata gatcgatgca acaggctggc tcccattcga tgcgttaccc taacacatcg
    29101 tcaggcataa gcgaagattg gacttgtgcg gggacgtgcg tgaggtcagc ggtgcagagg
    29161 aaaatgaagg aaaggcggga ccgtgcaacc ttgtcacggg tcccgccagc tgcggtcatt
    29221 acttatgctt tcggtttgac gttgagcacg acaatatcca gcacgatgtg ttttcccgcc
    29281 aggggatgat tgaagtcgac gacagccgac tcatccgata gggatacgac ggtaaatggc
    29341 cgaccttcag cagtcgtagc catatccccg cgggcaatgg tgtgcggcaa ttgggcgcgc
    29401 ggaacggtca tcttttttcc ggcgtcatat tgtccgaagg catggtctgc atcaagttca
    29461 acgcgcttgg tatcgcccga atgcaaacct acaagggcct cttccaaccc tggaaggagt
    29521 tgcttctccc catgggtgta ttcactggtt tggggaggga tcatcacatg atcgttgggc
    29581 agggtgatgg tcatttgcag tgtcactttt gaaccgtctt cgatggttgg ggagcctgtt
    29641 tgggcatgca gaggacggct cagcaggacg atagcacaca ggatcagcaa gccggccaat
    29701 gatacgcgtg tcatagatac ctcatctgtt gttagggata gaatacaagg tgatcaactg
    29761 acgattccgt tacggtgagt caatcttcac aattttggtc acacggttgt cgaagtcaat
    29821 ttccaggcta cacgtgtcgc ctttgtgtag accgctgagc atgtcagcgg aaagggcggc
    29881 cagagtccag acttcccctt cggcggtttt gaatgtcagg ctctggctcg cttggtcgac
    29941 ggattcaacc ttcacgcgcg tgtacgtcaa tgaagcctgg ctctctaggg gagcaacaat
    30001 cggcatggcg aggacacata ctagactgcc gatcacgatg atcgatttca tgatgtcatc
    30061 ctccttgtga caaaggcagg cgaacaatga ctcaccgctt tcaaaccgcg ggcctgaatt
    30121 gctactcaat gcatatggca gggagttatc atgttttcgc tgggtaacgt cgcggaaggt
    30181 tgacgagtgc ggacgaaccg gcgcgcaagt tgtccctgcg ggacatattg tcctctcctc
    30241 ggaccatgga aaagcagcgc aaatgtcgca ggatgatgca tccttcccag gttcggaatc
    30301 gggcggaata actgtcgcac tcgccttgtc tgcgatcgtg acgcttccat tgatgggccc
    30361 atggctcagc ggtcaatcgc ttcgacccta tctggagttt ccgccgctca gcacgggtgt
    30421 cgtgacgcag gtcgcatttt cctggccggt ttttgtcggt ctgtccgcgc tgattgtctg
    30481 cctgcttgcg ccgtttattg tgcgcgtggc acgaacgaac agcaggggtg tatcgacggt
    30541 agctgggcaa gagcgcacgc cgtatggctc cgttcgccgg cagtgtgttt tgccccatgc
    30601 ttttccctgg tggggttggg gtgctgtcgc ttggaccatc tgtgcatggt tcctggcctg
    30661 gacccgcttc gagtggatgc agccgtggca gccccatacc ttcactccgc tgtggctggg
    30721 ctacattgtc gtggtcaatg ccttgacgta tcgcagcacc ggccggtgca tgttgatcca
    30781 tcgtccacgg tatctgctct cactcttccc gctcagtgcc tgcttctggt ggctctttga
    30841 gtatctcaat cgatttgtgc agaactggta ctacgtaggt ggcggaacgt tgacgacagg
    30901 ggagtacctg gttcgcgcga ccctcccgtt tgcgacggta ttgccggccg tcctcagcac
    30961 ggcggagtgg ctcacggctt gcccgcgcct ctcggcggga ttggaccatg ttgtcacgat
    31021 caatttcagt cgccgggtat ggggatggtt gctgctatcc ggcgctgctg ccggtctgct
    31081 cggcatcggt ctgtggccgg actatctgtt tccgctggtg tgggtggcgc cgctcctggt
    31141 gatcacggcg ctgcaactga ttcgcaacga gccgacgatc ttcagcgaga cggcgcatgg
    31201 tgattggcga acactctggg cggtggcgct ggcagcgtta gtctgcgggt ggttctggga
    31261 gatgtggaat ttctacagcc tggcccattg gcaatatgcc gtaccctttg tgcagggagt
    31321 gaccctgttc cacatgccgt tgcttgggta tgccggttat ctgccgttcg gcctggagtg
    31381 ttgggccgtg gccgacttgt gtctcgccag gaagtttgca ggaggcgtgg cctattatcg
    31441 tgcggctgaa ccggagacgg atttcgcgca acggaacccc acggtagcag aacgctgatc
    31501 ggcaggtgcg ccgctgcgag ttgccgttcg cagcatgatc gggcggcttt tccaggaacc
    31561 accgcgaagg cttttgtatc ggccgctggt cgggccggga ggattacgct ccggcatgta
    31621 ggcataatag tcgaaaccga accagtcatt gacccattca gcgacgttgc ctgccatgtg
    31681 atgcacgcca tagtagcttt tgcccggttc gccgctgctc accggcgcca gaatcggaat
    31741 ctcgtgcaca tgatgctgcc cgaacatggc cagggaattg tcggggagcg actcgcccca
    31801 gggaaaacga ttcccgtccg gcccccgcgc cgccttttcc cattcggctt ccgtcggcag
    31861 ccgtttgcct gaggcacggc agaggttgtc ggcttccttc cacgtgacat acagggcagg
    31921 ccactgcgcc aaggtctcat cgctgaccga gtgcacggtg atgacgtgcc agatcaaatg
    31981 ctggagatct ttggacgggt ggatctttcg ctgatgcaga taggtcagat attccccgag
    32041 gctgacttca tcacgatcga tctcgtaggc atccaaccag acgcgatgct gcgggagctc
    32101 cgtatcgtcg aactgtgtgc ccatgccata ggggtcgtcg tccactcgaa tgctgccgag
    32161 taagaacggt ccctcaggaa tgagaacggc cggtgagctg gccgctaatt ccgcaatcga
    32221 cgtcaggtgt ttggccagct ccgctgttgg cggaggggtc ggggcgcgag cggcgatgga
    32281 gagcgccgga ttgagccatg tggccgcaac gctcaggatc acgagactga gcgcatgcag
    32341 gagtgaggcg attcctggga acaacgtgcg agggatcatt acaacttact cggcgaggcg
    32401 acggcttttc cgatttcggc aatgagccgc cgttcgatct ccgcgatctc ctcagcatcc
    32461 accgccagga cgcgcccgcc ttgggctacc cgttcaaact cggcttttac gctcaaccgg
    32521 gtgacgttcg aggggagggc ttgaagggat accacatact gattgcggga gccggccgtt
    32581 tccaccgagg ttgggatgga aatttttcgc tcggtgacga agaccgcttt ttgatcggtt
    32641 ttgactgtga cgcgtatccg gtacccgttt cgcgtcaggg cttcttccgc agctttagca
    32701 acggccggca gcggacgcgg gagatcctcg acctgcgagc ctttttcttc cacccggata
    32761 ctgtccgtcg cctgggcggc tttggcggcg gtccgttgaa tctctccgga gagttttttc
    32821 gattcggcca gttgcgcatc gagcttcgtg ccgagcgtct tcgcttcggt ttgcagcgac
    32881 ttggcctcag cctgggcatg ctccgtctgc ttcttcgcgt cccggagttc ctggttgagc
    32941 agatcgatct gctgctgcat gactttgtta ccgtcctgca gcgaattcag cagcgattcc
    33001 tgcttcgcca gttgcttccg gagattttcg ttttcctgcc ggaatggaga ttcgtcgggg
    33061 gtacacccga cacaccacga tagtaccgcg aggctgaaga ggggggcgag cgatcgctgg
    33121 gaggccttga tcatagtttg ggtaagacaa tcagactagt atcagagttg agcccccctt
    33181 gtctatcttc aatcactttc tcgccggttt gaccatggga aatggagcgg ctattctgtg
    33241 ctcatgcggt actggtacga ggcggttttc ctcatgagtc tggcattgtt tcagccggcc
    33301 gcggcggcgg tttcgatcgc agcgatggct gaaccggcgg agcccgtgcc tgccgaggac
    33361 tatgcgtttt ttgacctcgc ggtcgaggac aagtttctga cgtcccacac gaagttgatc
    33421 gtcatcgaac ggatgacggc gacgcatctc cagccggatg agccgcagct gccgaccctc
    33481 gcctggtttg ccgagcgcga atggttcgat ggccgtttgc cccaggacct ggtgcgggat
    33541 ttcgtgggca agaatcagcg gccgtcgcgg cttgatacac gatttagttt cggcgtgcgc
    33601 taccggttta tgatgggcga aggaggcggc gatgccgaag cctctgtgcc tctgattccc
    33661 gccacctggt tagttcaaga agaaggcggc gcccccgacg tcatcgacca tctggcattt
    33721 tcgcgggtgg gtctgaacct gcgagccgat caggcgttgc tgtatgtcgc gaatccgcgt
    33781 cccgatggga ccggtgcggg ctttttgctg tggtttgtgc ggcaacagaa ggtctggtcc
    33841 ttgcacgaca cagaagtgtt gtgggtttca cggccggacc aaggtcaatc aggccgccgg
    33901 taggatgact gagacgttca ggttcctgtt cgattcactg aggagtgcgc atgattcgta
    33961 agacgttttc tgttccacca ctcgggtgta actgctcgat catcggggac cccgtcacca
    34021 agcaggccat cgtggttgat ccgggcggcg cgcctgagcg catcctgcaa gaagtgcgtg
    34081 cgatgggatt gacggtggtg agtattgtcc atacgcatgc acacttcgat cattttctcg
    34141 cgtccggcgc catgaagcag gcgaccggtg cggcgttgtg tctgcatcct gacgacctgc
    34201 cgctctggga cattttggag atgcaatgcc gcatgttcgg cgtgccctat gttccggtgc
    34261 cgcagccgga ttattggctg aaagatgagg agcgtgtggc cgtcggcgag atggaaggcg
    34321 tggccttgca cacgccggga cacacgcctg gatccatgtg ttttcacttt cccgctgcaa
    34381 aattgttggt ggcgggggat acgctgtttc gcggtggcat cggccgcacc gatttgtggg
    34441 gcggggattt cgatgccatc gagcaatcga tccgggaacg gctctacacg ctcgatgcgg
    34501 acacggccgt cgtaaccgga catggggctg agaccgagat cggccttgag cgagagacga
    34561 attcattcgt gcgcgcctag cggagaggtg ggcatttcac ggtgcgtgaa ccttctgcgg
    34621 ggcgcgagat cgttccccga cagcctttgt catagccgct catgaacggg cctccatcgg
    34681 aaacacagga gggtattatg atcgcgacgg ctcggcgctg gttggtatgt ccgctgatgt
    34741 ccttcctgct gggattctcc gctgccgatg tgctcgtgta tgccgacgaa cctgggtctt
    34801 ctccccaggt cacgatcagt caggtccggg tgggattgtc cgatcacggc ccggtggtgt
    34861 tgctatcagc cgacgggaaa accattcccg tctttgtcga ccacaccgtc gccgcgtcca
    34921 ttcaggccgc actcaccggt gaaaagcttc cgcgcccgct ttcccacgag ctgatgcata
    34981 cgattttaga atcgctcggc ggacgggtgg tccggacggt gattacgctc aaggcgggca
    35041 cctattacgg aagcctgacc gtggcgtttc aggggcagga aaaggtgttc gatagtcgtt
    35101 cttcggactc catcgccctg gcgattcatt ttcacgcgcc gattctggtg gcgcgggaca
    35161 tgctggatgc ggtgggcaag tcctcgctgg agccgaagcc cgaatcactc tgagctcgcg
    35221 cccgacggaa catcgcggga ctctcgcgag tcgcgtagaa gtatacagtg ctggcctttt
    35281 gtccggttct atcctatcgt actgctgatg cgcagaggcc tgaggttgtc acaggtacgg
    35341 aggccacgtt tccatgaaaa accaacgcaa gcatgagcgg gtgcacgtcc agtttcgcag
    35401 tcatttttcg atgaaaggca agatgctggc cggtgatgga gatctcaccg atctctctcc
    35461 gggcggctgc cggattatca gccctgtcga agtgctggtc gggtcggaag tggagctctg
    35521 tattttcccc ggtgacaatg ccaatcccat cctcatcgat gcggccaccg tacggtggtg
    35581 ccgacccaac gagttcgggt tgtcgatcac caagattcga gggccggtcc agcgccaatt
    35641 gaccgacgtg tggcgcaagt tggcgaagcc tgcttagcag gctgttgata caggcgcgcc
    35701 agcggcgttc tcacctcgct cagaggctca ccgtacggcg cgagtacgat tcgcctcttc
    35761 gctcgcttcg gccttgctgc acggcctttc tgaacagcct gcacggcgcc agacgcaact
    35821 gcgcatttca cgcaacgatc ctcttccgga tgccgcgtac agttgatcaa cgcgctgcta
    35881 gtcgagtgtt attccagaag tgagttgccc aaatcggata ggcgtggcta gatcttcatg
    35941 gcctcccgca cttcggccat cgtctgagaa gagacggcgg tcgcgcggcg tcgcccgtcc
    36001 tctgcaatgt catcaaggcg ccccggatgt tgcgtgaggt tcgcacgagc ctcccacatc
    36061 ggagccaggc gctccaccac gcgatcggcc accagcttct tgcaatcgat gcagccgatg
    36121 gcggctttcc ggcaatcctg attcacctgt tcgatcaccg gcagcggcga aaaaatcttg
    36181 tgaaagtcgt agacggggca gacgtcggga ttcccggggt cggtgcgccg gacgcgcgcg
    36241 ggatcggtga tcatggtctt gagcttctgg cggaccaccg cttccgtatc ggagaggttg
    36301 atggcgttgc cgtagctctt gctcatcttg cgtccatcgg tgccgaggac tttggggaat
    36361 ttcgtgaggt gctcctgcgg ttcgggaaag acggagccgt aaaggctatt gaagcggcgc
    36421 gccagttcgc gcgtcagttc gagatggggc aattgatctt tgccgaccgg tacgaaatcc
    36481 ggcttgtaga gaagaatgtc cgctgcctgg aggaccggat agccgagaaa cccgtaggtg
    36541 ctgaggtcgc gctccttgat ctcttcctgc ttctccttgt aggtcggatt ccgctccagc
    36601 caggacaccg gaatgatcat ggagaacagt agatgaagga tggcatggtc cggcacctgt
    36661 gactgcacga atacggtggc ccggttcggg tcgatgccgg ctgccagcca gtcgatgagc
    36721 agctcccgca catattcgcg gagccggctg gtgtcggcgt agttggacga caacgcatgc
    36781 cagtccgcca cgaaaaagta acactcgtat tgagcctgga gcgtgttcca gttttccagc
    36841 gcccccaacc agttaccgag atggagcagc ccgctcggct gcatcccgct gagcactcgc
    36901 ttacgcgtcg tcgtcatgcc gtcactccca tatcccgaac cgatgtcttg ctgtctcccc
    36961 gagcagcagg gctactccag ggggacgaac ttgccttgtt tcacttgaat aagaaagacg
    37021 tgtcgattga gggtgccgtc gggattaaat ccgctggggc cgctcaaggt cggcaggtca
    37081 tgttgcatca tcaaatagtc gcggaccgct tctccgctgg ttgcgcctcg ccggatcgct
    37141 tccacggcca gtcgcgcggc gtcatagcct tgggcggcaa agagtgacgg cgtcgcctga
    37201 aaccgtttgc gataccgttc cacgaattcc tgtacgatcg ggctggtgct atcggtaaaa
    37261 aatccgtcgg taaacacgct gccctcgaca ctgcgatcgg ccacgcgcgc gaaatccggc
    37321 gtgttccatc cattggtgcc gagcagtggt acggcgatgt cgtaaaaagc aagctgggcc
    37381 gccagcaggc ccacgtctaa cgaacgtccc ggaatgaaga ccgcatcgaa tccgggcgag
    37441 tagaggaggc gttttccctt cttgcctgcg ccctgcctga tcccggtttt ggccggatcg
    37501 ttgtcgactt gcacctcgac gccgtacttc ttcagatctt cggctttcag cctcccgatc
    37561 accgcgcgga agtcggtgtc gccttctttg taggcttcgc tgacgatcac ttctccatcc
    37621 tgctgccgga tttcttgcgc gaagaggcgt gccaggtcgc ggccataggg cgtatcggga
    37681 tagagaatgg caaaccgttt gaactgctgc tctttgacgg catagtccgc cacccgtttg
    37741 gcttgatgtc cgtacgtcag tgcggtgctg aatacgtagt tgccgaagcg gcgtaagttc
    37801 gggacggtcg cgctgggggt gatcaaggga atgtgggtgc gttcggccat ttctgccatg
    37861 accggcaggt ttttcgacag caacggcccg atgacggcaa gcgggcggtc gtccgacaag
    37921 acagcagaca gttcgtcgag gaatgccgcg cggtcggatt ctgtgtcctt cacgatgagc
    37981 ccgatcgacg gagtttcacc gccgtccttc gttcgctcca gcgcgagttg aatcccgttc
    38041 aacacttcgg tgccgaaggg cgcgagtttt ccggacatcg ggaaaatggc cgcaatcgag
    38101 tacgcgtgcg atttaaactt ggtccgcacc acgctttgca ggtcggcagc cttcgcggca
    38161 tacgggtggt tgggaaagcg gctcagaaag aggcgcacgt cccgctcgac taagtggtct
    38221 tctcccgccg ccgtatgaag ttcgatgagc ttgatggagg ccaagtctcc tgggaaggtc
    38281 ttcgggtacg catcgcggac gcgcatgagc gtcggcgcat ccagtttgtc attgaccaat
    38341 tctcggattt gagtttccgt ctcgcgcgac tggtcgccga catccagcgg cagctcatcg
    38401 agccatgctt gaatggcccg cagaaagtct ttcttctgaa cctgaaactc gcccgtcagc
    38461 cgcaacgcat cgcgtttgat ggccggatcg gcggacaggc tgcgcacttc ggacagcagg
    38521 ggcagtgcga gatccaggtt gccggctcgg gcgtgggttt tcgcgagcag gagtttggcc
    38581 cggtcaacta aatcggagtt cggaaactct ccgagtagct ggttgacgta gcgaatggtt
    38641 tccgcatgtt ccttcatgcc gtacatggcg gcggccatga gcagataggc gtcgtcgagg
    38701 agatccggac cggggccgcc ttcgatgaat cgtcgcagca tgacggccgc ggcctccggt
    38761 tgttcggcgt cgatcaagcg cttggcctgg tcgatggcgg attgtccggt attcgcaggt
    38821 ggcttcgcct gattcgaacg gggtgcggcc ggagtcttgg gtgccggagc tgtttcgccg
    38881 agcgtggcaa agcctacgag gagcgccacc gcgagggcga cactgcacgc gcggagatgc
    38941 ggggcgcggg atactgaacg aggaaccatt gactcctgtg catggcgaac gctctcgccg
    39001 caatggaggc tactatagga aacaggtgct agggtgtcaa ggtaagtcga ggcgtttgcg
    39061 ttgtgttcac agagaggctt ggctatagtc gacgagacat gcttcccgcc atcaccgatg
    39121 agggcattgt tgaagagccg tgatgcggcg agtcacgctc cgcaggaatc ggattcgctt
    39181 cccttggcgc cgctcgttgc gcggtattgc gatgatttgc gcatcgcgcg gggactctcg
    39241 cggcatacgc tctcggccta ccagcgtgat gtgacggagt ttcagcgcta tcttcgcgat
    39301 caacagatcc atgatcctgc tcaggtgtct cctccattgc tcgcggcctt tctcgagcat
    39361 ttgcaccgtg gtggtcttgc ggcttcgtca cgtgcgcgag ccttggccgc agtgcgtagt
    39421 ttgtttcgtt tcctgaagca ggaaggcgtc gttgccgcga atccgacggt cagtctgcgg
    39481 agcacctctc gagcgcggca tctgccgaag acattgagcc tggaggaagt ggccagtttg
    39541 ctggatctgc cgcatcgcgg cgcgccggaa gatcatcgcg atcgtgcgat ggtggaagtg
    39601 ctgtatgccg cgggtctgcg agtgtcggag ttgatcgcgt tacgcatgga tcagtgcaac
    39661 cttgaggtcg gctacgtcgg cattaccgga aaaggcgaca agcagcgggt ggtccccatc
    39721 ggcaggcccg cggtggcgga agtgcaggcc tatgtgatga gtgcgcgacc catgctgttg
    39781 aagcaccgat cctcgcgctt cctgttcgtg acccgccgcg gcacgccgtt gacacgacag
    39841 gccttctgga agctgctgcg tgcacgagcc cagcgagcgg gaattgcgcg catgccgtcg
    39901 ccgcatatgc tgcgccattc gtttgccacg catctcttgc agcggggcgc cgacttgcgt
    39961 tctgtgcagg cgatgttggg ccacgccgac atcgcgacga cgcaaattta tacgcatgtc
    40021 gatgcggcac agttgaaaaa aatccacact gcgtgttttc cgcgccacaa atcccgccgc
    40081 tgaatgtccg atggcgcgag ccgtcgtgcc aaaggggcct gatctgcgta ggataaaaat
    40141 cttgcaagaa taatgcatga ttcttggttg acactgcttg gtggagttga tagcatccaa
    40201 aaaatctgcc gagactgaga aaaaacgggc ggatagctca attgggagag cgctgccctt
    40261 acaaggcaga ggtcacaggt tcgatccctg ttccgcctac caataagaaa tacaagaaat
    40321 tgtgcgcggg gcattgcctt atccattagt agtgatgtac aagagtacgc gctactgtat
    40381 ttagacagtt tgattctgaa gatctgaatt cttcttccgc catcttgggt tgcgtggggc
    40441 cgtcgttcag cctggttagg acgccagatt gtcaatctgg aagtcgcggg ttcaaatccc
    40501 gtcggccccg ccattccccc agcttttggt acaatccatg ctaccgtttt catgcgacgc
    40561 catgtgaagg gaaaggtcta ggctcatgtt ccagtcaggc gtaatggggt tggtgggctc
    40621 gctgggagcg gtatcgaaaa tcgtgctcct gctcctctta gtcgcctcga ttctttcctg
    40681 gggcgtgatt ttgtacaaat ggcggacgtt caaggcggct gatcgtgaag accagcggtt
    40741 cttcaatgcc tttacgaaga tccgtgatct ggatgagttg tatcggcagt ccaagcgcgc
    40801 agagggcagc cccagtgcgc gcgtgttcca gggaattatg gatcgcgtgg tgacggcctc
    40861 gggagacggg gcggtgagtc cgtcgctggc cgcggctgga tcgaaagaat cggccgccgc
    40921 cttcgatcac cagtacatgg ataaaacggt gtcgtacctc gtgcagaacc aggtctctca
    40981 tttggaggcc tacctgccgg tgctggcgac cacgggcaac attacgccgt tcatcggctt
    41041 gctcgggaca gtgctgggta tcatcgactc gtttcgtgag atcggcatgc agggcacggc
    41101 cagtattgcc gccgtggctc cgggcgtatc tgaagccctg gtggcgacgg ccgccggcct
    41161 gttcaccgcc atccctgcag tcattttcta caactattat ctgacgcgta ttcggaagac
    41221 cgtgtttcgc atcgagtcat tcaccgtcga agcgatgcgt tcattgcaga cgcgcttgaa
    41281 gcagccccag gccggagtgt agtgatgacg tcggaaaccc gccatcgccg gttcatggcc
    41341 gagatcaatg tgattccgct ggtcgacgtg gtgttggtgt tgctcgtcat tttcatggtg
    41401 acggcgccga tgctgtatcg cggcatggac atcaatcttc ccaagtccgc gagcaatacg
    41461 attaagcctg aagcaagagc ggttttgtcc attgagcgcg accagcgatt gtatttggat
    41521 aaggacgcgg tgagtgtcgt gcagttggag cgaaaactcc gggcgctcaa agaccagagc
    41581 ccggatgtgt cgctgtatct gcgtgcagac cgggatgtgc cgtatgggat cgttgtgcag
    41641 gtcatggata gtgtcaagaa agccggcatt gaaaagcttg gtatggtgac ggagcccaca
    41701 ggcgccgagc gggtcaatga gtcggttgcg actccctcgc agccacgcaa gaagtagcgg
    41761 cgccgcgact cgcgcgccat acggtcgtat cgcaagtcat gacgttccaa gccctgccaa
    41821 gacataccgc gctgtcccta atcggagata tgggtgaggc cggaggtgac cgttttcgaa
    41881 agacggtcgt cgtgtcgtta cttctgcacc tgtgtctcct ctccatcatt atgggggcca
    41941 agttctttaa aaaaacagaa cgtccgcttt cggcggtgga agtctctctc gtgacgttgc
    42001 cgcctgtgga agcgaagcct gaaccgcaag tggagaaggt ggagaaggcg gtaccgaagc
    42061 ccgcacccaa gccggtgcaa tcggctccga ttcccgtgcc tcctaaaccg gtgcaggccc
    42121 cgacgccgac tccgccacct gttcaatcgg cgcccgttgc gaagccggca ccaccggcgc
    42181 cagcacctgc gcctgctccg gttcccgttc ccaagctcga ggctccggta ttgacggcac
    42241 ctcaaccgat tccgcaggcg gccaagtcac cttcttctgc ccccgtcacg aatcgggcgg
    42301 atgtgttgcg tgatgtcatg aaagatgtgg aactcccgct caatgctccg aaatatggag
    42361 acattgcgcc gatgaagccg gcggaggtca agaaatctgc gcaggcgaag ctcggagcgc
    42421 cggaacgctc tgatctggac gccatgttga agaagctgaa cgtgcctgat atggctgcgc
    42481 ctcaggtggc ggttccacaa gagcccccaa agccgaacgt cacgccggca aaacgcgcat
    42541 cgctgtcgga ggaaatgacg agcgatctcg atcgtgagct tcaggatttg aagaaactgc
    42601 aggcgcctcc gcccgtgaaa gcaacggagc cggtgcgaga agtgaagccg atgttccgcg
    42661 aaccgcaacc gccggtgacc gcgccgccgg tggcggccag tgtcacacgc agcaagccca
    42721 ctcccatgct gcgggtgccg ggcatgtcag ggtcgaatcc gtatttggcg agggttcagg
    42781 cacggatcag cggattctgg acggcgccgc cggtcgatat ttcaggcaag gctttgacgg
    42841 tgacggtgcg gtttcggtta gagcgggatg gtcgggtcgg gtcggtcatt atcgagcaat
    42901 cttcggggaa cgactattac gacatggctg cacaacgcgc ggtgcaaagc gcgattccgt
    42961 tgccgccctt tccgcccgac ctgacggatt catatttcga tgctcacttc acttttgccg
    43021 taggcgaggc tgcaggatga atcgtgtgat catcggtctt atgttgaccc tctgtgtcgt
    43081 ggtgggcgcc ggactgttcg gaattctgga ctcccgcgcc accgatgtct ttcttgaggc
    43141 gacccggcct gatttccaaa agatccccat tggtgtgttc gggtttcaga acggtggcgg
    43201 cccggaatgg ctcggggggc gactcgaaga agtgttgaag gccgaccttc aacgatctct
    43261 tgtgttctcc ctcgtggatc tgcccggaat cggtgtgaag gcccgcgagg tgtcgaccac
    43321 cgataaggcc gtgttcaaac aagcggctga gaatggggtg tccgtcctgg tatggggaaa
    43381 gtccggccag aagaacggca gtaaggacag cgaactcctg atggatggct tcgtgtatga
    43441 cagcggcagc gacgaggtcg tcggaggcaa gcgatacgtc gggtcaacat cggtcgtccg
    43501 gttgatggcc catcggtttg ccgacgagtt ggtgtttcgt tataccggcg agccagggat
    43561 cgccagaacc aagattgtct atgtcgcgga gcatgggaat gcccgtgaat tgtttgtgat
    43621 ggactacgac gggtatgagc cgaagcagat cactgccgac ggctttctga atctcatgcc
    43681 ccgatggtcg ccggatcgcc ggtttattgt cttcacggcc tatcggagtc ggaacacgca
    43741 ggacatcgat attctggagc tcgcgacggg gaagcgctgg acgctggtgt cgatgcccgg
    43801 gctgaacatc accccggcct tgtcgccgga cggaaattat ttggcctttg ccagcagcca
    43861 ggacggcaat tcggagattt ataagctcga tacgcgaacc aagactccgc agcgtctgac
    43921 cgtgaatcag ggaggcgatt tgtcgcccac ctggtctccg accggacggg aaatcgcgtt
    43981 tacctccgat cgcggcggcg ccccgcaagt gtttgtcatg agcgccgatg gctccaacgt
    44041 ccgccgcttg acgtacgagg gggattacaa tgccgcaccg gcctggtcgc cgcgcggcaa
    44101 ttggatcgcc tatgtctgtc gaaccgccca gcggctgtac aaactgtgca tcgtgtcccc
    44161 tgatggtcaa aaacgggtgc aggttacgac gggcccgggg atcgacgact cgccatcctg
    44221 gtctcctgat ggccgccacc tgaccttcag ttcaaccgtt gacggaaaga gccacatcta
    44281 catggtggat acggacggaa aaaatctgga acgcatcaca ttcggcggta cacacaatag
    44341 ttcaccgtca tggtctccgg cactttagga ttgagtacgc aattgacagg atctacaggc
    44401 atactaacca ttcaccagcg atgaggagtg acgatatgag gatacgggta gcgacaatgg
    44461 gcctgaccat ggtagtcggg atgctgttgg tcatgcaggg cgggtgttcc aagaaatcga
    44521 ttcaatccgg tggcgatgcg cagtcctctg atcgcgggat ggcgaagtcg ggcgctccgg
    44581 cgcagtctca agctcaactc ccgcctccga cagggttgga tgctcccagc gccaccttcc
    44641 cggacctgtc cctttccagc aaaccggaag atcctgagac gggcggcttg cgcgggttcg
    44701 attcagtgtc gggcggcaag gctccgtctg aggagcggct tggaggcggc ggcacgatgt
    44761 tggccaaggt cgagccgtcc gaaagcacgg cacgccagat tgaagaaatt cgtcgggagc
    44821 aggcgaagga gcaggcggcg tcggcggaag ccggattgcg cgacgtgttt ttcggctacg
    44881 atagctggac gattacggaa gatggccggc agtcgttgac gcaggatgct cagtggatca
    44941 aggcgaatgc cggggcgttg gtcaagatcg aagggcattg cgatgaacgg ggcacgctgg
    45001 cctacaatct cgtgttgggc gaaaagcgag ccaaggccgt gcgaaactat ctggtcgaac
    45061 tgggcgtcgg cgccaaccgg ttgtcggtgg tgtcgtatgg caaagagcgg ccgttctgca
    45121 acgagcgtag tgaaagctgc tatcaacaaa accgccgggg gcatgtggtg gtccggtcga
    45181 aataactgtt ccaatcatgg atgatgtgac atgattctcc ggaacagacg ccggtcaagg
    45241 gagacctgtc atggacagtg aatggcctta tcgaaagacg atgactgatc aaccgtcgaa
    45301 cgcgaagcgt gtgacgccgg gcacctatgc gcaggcagct cgattcggtg cctgtgtcgt
    45361 ggtcgtgacg ggctgtgtct tgttgtcggg ctgtgtggcg cagcaggcgg atctcaagca
    45421 gaccgaacga gagctccagc gtcgcatcaa gcaacagacg gaagagcagg cccagaaccg
    45481 ggcacgccaa aatcaggaaa tcgtgtcgtt gcgcgagcag gacattccat ccctgcgcgg
    45541 cgatgtcgat aaggccctcc accgtgcaca aagtttggat gcgcggcaag acgacctgct
    45601 ggccaaggtg gccagccagg aatcgaagtt cgagcgtcgc atcggcgaag ccgagaagcg
    45661 gtcggctgag gaaggcaagc gcttgggctg ggtcgaaaag cagctggtcg atcaagatgc
    45721 catgctcaaa ggcgagcgag accggaaccg ggctgagctg gccgcggtga ccgctcgtct
    45781 ggatcaaatc aacagccata tcgaggccat tcagaagaac gttctggatg cgatgcagaa
    45841 aactaccacc gggctcgcgc aaaaggtcga ttcgcgcctg gatgaacagc agaagttact
    45901 gcacgggttg gagacccgtt cccagaacgt cgcgcagctc gatgcccaga ataaggtctt
    45961 gggcgatcag gtcgggaaat tcaatcaggc actggtcgac ttcaagcagg cgctgggaag
    46021 cttgggcgaa cgcgtggtgc aacaggacca gaccgtcaag catttagccg cgaccctgga
    46081 gcaggatacc gtggctttga gcaagcggac cgatgcgctg gccggaaaaa tggatgccga
    46141 taacaaagtc accgccgacc acttcaatga ggtcaatcgg agtgtcgcct cggtggcaaa
    46201 ggcgctggaa aatgccggcg gcaaatttgt gtcccgtgag gacgaccatg aacggcgcct
    46261 cgatgacacg actcgagaac tgacgcatgt gcaggcacag atccagacgc tggacaagaa
    46321 tctcgaaaat caacatgcgt tcctgaagca ggtcgagcag catttggtcg ccctgcgagc
    46381 gaacgccact cagcgcagcg aaccggctcc cgccgtcgcc gaagcggcac cgcttcccca
    46441 ggcgattccg gccccggttc cacccccggc cccggcgcag acacaggact tcgcatcgtc
    46501 atcggctatc accccccgtg cggaaaatcg tagcgcggcg ctggtggcgg atcgggagtc
    46561 ctacgaacgg actttgaccc gtttcaagga tggcgatttg gatggcgccc gtcaggggtt
    46621 tgcggagttt ctcgttcagc atccgcattc ggacctggcc cccaacgcgc gcttttggtt
    46681 gggcgagtcg tactatggca aaaaggatta ttcgcgggcc atcgatgcct atgatcaagt
    46741 gcagttgaat catcctgcca gcgaaaaggt gccggccgct ctcttgaaga aagggtatgc
    46801 ctatctggcg ctgaaggatc ggaaaaaggc ggcgtccgct ctgaaacaag tgattgacct
    46861 gtatccgagg tctccagagg ccaataaggc catggataaa ttgaaccaac taaaggagtc
    46921 gcactaacga tgaagctccg attcgcgggg gcattgcggg tgagtgccat gctggcttgc
    46981 gggctggttg tggcgggctg tgcaaaacat gctgatttcc tcgaaatccg ggatcaggtc
    47041 tccatcatgg cccggactca ggatcaggag cagaagcggt tcgaggcgat gcagcgccgg
    47101 ctggaatcgc tcgagcgggt gcgtgagccg gaaggcggga aactgcgttt agacgacgcc
    47161 atgacccggc ttcaaaagct ggaagggcgg ttagcgaagg tagaagaggc gcagctcgct
    47221 caagcggctg ctatccggtc tgatgccgct ctcgcagaag ccagccgcca ggcgcgtatc
    47281 tcgaaaccct caggcccgat tgaacctcaa gccattgtgc cgggagttcc gtcgatcacg
    47341 ccgacctcgg cgttcaatct ggcgtacaac gactacctca acgggaagtt cgatttggcc
    47401 gtcaacgggt ttcagcactt catcaaggat tttccctcta cctcgctgac acccaatgcc
    47461 cattactggc tcggggagtc ctactacggt cagaaggatt atatccgtgc catacagtcg
    47521 tttgagcacg tggtcaatga gtacgcggga aatgagaagg tgccggctgc cctctttaag
    47581 ctcgggctct cggcggctga aaccggagat accgcaaaat ctcgcaagta tctcaaacgg
    47641 gtcattgagg agtactccac atcggacgaa gccaagcttg cgaagacgaa gatggccgag
    47701 attcgatgac cctgaggtgt ttgtccggtc tcctctccgc tgagttatcc tcccatcaac
    47761 tcgcaggggg cgtttcttcc catggatata gccagtagcg tgggaaagat tcaggttctc
    47821 cccagcgatg tcatcggacg catcgcagcc ggcgaagtgg ttgagcgccc ggctgcggta
    47881 gtgaaagagc tcctcgaaaa cagtctcgac gcgggcagta gcgcgatcac cgtcgaaatc
    47941 aaagacgggg gcctcggatt gattcgggtc agcgacgacg gcgaaggcat gtcgcgtcgc
    48001 gacgcctctc tggccttcga gcgacatgct accagcaagc tccagtcgga tgaccaactc
    48061 ggcgcggttc gtacgatggg attccgcgga gaagccctgc cgagcattgc cgccgtctcg
    48121 aaagtccgtc tgacgactgt ggcccgtcac gatcaggtcg gcacacagct gtggcttacg
    48181 ggagggaccc tcactcgtgt agaggatgcg gccgcgatcc ccggaacgac gatcgaagtg
    48241 tcggagctct ttttcaatac gccggcccgc cgaaaatttc tcaagtcgac caccacggag
    48301 ttctcccaca tcagccatgt ggttcagcag gccgcgttag cctggccgca ggtgtacatg
    48361 cggctcgtgc ataacggata cgaagtgttt gcgctgcctg ctgtgtcctc gccgcgcgat
    48421 cgcgtgctcc aggtgtatcg ggcagcgttc ggtgagcgag ccttggctgt cgatgtcgag
    48481 cgcgatggtc tgttgctgaa agggttcatc attgatcccg tccgggcccg tgcgggacgg
    48541 acgccgcagg aattatttgt caatcgtcgc cccattaaga acagcacggt gcaacatgcc
    48601 gtcatcgacg gctatagctc gttcctggcc aagggccatg caccgctctt cgtgttgttt
    48661 ctggatgtcg agccgcaccg cgtggatgtg aatgtgcatc ccacgaaacg tgaggttcgg
    48721 ttcgccgata ccgagcagat ccaccagctg gtccgctctg ccgtacgcca gacgctcggt
    48781 cgggcacagg tggaagcgtc gatggcaggg gcggctcatg ctgagatgac cggtgtgatc
    48841 ccgccgactc tctctttccc agaaagggaa cggatgtcct ttggggaagc gccccccggg
    48901 ccaatggcca gatcgaaggc ttccgtcacg ccacctgatt ccgctgagag ccaaacttct
    48961 ttcgttggag aaggcgccac ggcgtatgcc tcacctgcat ccacagatat cgttgcactc
    49021 gggcaaatga accgtacgta tttgattgct caggtcggga acgaactcca ggtcgtcgat
    49081 caacatacgg cgcatgagcg tgtgttgttc gagcgactct ggcgagcgtg gcaggggcgc
    49141 gcggtgacgt cccaaccact gcttttgccg gaaccgctcg agttgccggt gcagcaagcc
    49201 gtgattctgc agcgacatct accggagttg gaacgactgg gattgctgat tgagccattt
    49261 ggaccttcat cgtttctgat ccggagtctg ccgatcatgt tgggtcatcc tgatttgtcc
    49321 gccttggtgc aggacctgat tgatgatctc gaacagtggg attccatttc gtcgctggaa
    49381 acgaaggtga agcctattct ggcgtcactg gcctgtcacg cagcggttcg cgcgggccgg
    49441 tcgatggcat tgcccgagat cacccagctg gttcaggatt gggtcgcgga aggcctgatc
    49501 atgacctgtc cacatggacg gcgtgtggcg ttccgtctgt cgggagacga actggcgcgt
    49561 ctgttcgatc gcgcctaggg gaagcacacg gagagatatg atccggctca cggaatccgt
    49621 cctggcgtcg cggccgctcg tggtactggt cgggccgacc gccgtgggga aaagcgacgt
    49681 cggtcttcgg ctggcccgcg cgttggacac cgacctcttg acggcggact cgcgccaggt
    49741 ctatcgcggg atggatatcg cgacggataa gcctgccgtg gcgcagcagc agggggtgcc
    49801 gcatcgactc atcgacctcg tcgatcctga tgaatcgttt aatgccgggc agtatcgcca
    49861 gctggcgttg caggaaatcg aacggctgta cggagaacgg cggctcccat tgatcgtggg
    49921 cggcaccggg ctctacgtgc gtacgctgat tcatgggctt tgtgatgccc cgcgggcgga
    49981 tcaggcgttt cgtgactcgt tactgcaaaa agctcagggg cagggacgat ttttcctcca
    50041 tgccgaattg gcgcaagtcg atcctgaact ggcggcaaga ctgcatccgc acgatgaagt
    50101 gaaaatcgtg cgcgctctcg aggtatttca tttgtccgga cggcggctct cggatgtcca
    50161 gcaggaacat cgctttgccg aacaaccgtt ctccgtgctc atgatcggat tgaatcgcga
    50221 tcgcgcagaa ctgtaccggc ggatcgatga gcgggtcgag gcgatgtttg ctcgaggcgt
    50281 cgtcgaggaa accgcagcgc tgctggcgaa gggatacggc cgcgagacgg gtgccatgaa
    50341 ggggttaggg taccagcagg tcgcaggcta tctgtccggt gactatgacc gcgcggaggc
    50401 gctccgtctg ctgaaacgcg ataccagaca ttttgccaag cgtcagctga cctggtttcg
    50461 aaaggagcct ggtctgcagt ggtggatgct gaccgagcag gataccgcag aggacgtggc
    50521 cgggcgtctg ctggaaacca ttcacacgtt tgtacaggca ctgaatgacg agcgccagcc
    50581 gcatcggcct gcgccgctta ccatagaaat ggaatcgaca tcatgaagaa agcaaagaag
    50641 accattcagc ctgctgtcgg tattatcggc gggagcgggt tgtaccaggt cgaaggatta
    50701 gagcaggtcc gcgaggtacg tctgcgcaca cccttcggcg caccatcgga tgccattgtg
    50761 gtcggagtgc tgggcggcat gggagtggcg ttcctttccc ggcatgggcg cgggcaccgg
    50821 atcaacccca gcagcatcaa ctatcgcgcc aatatctatg cactaaagtc gttaggggtg
    50881 acccaagtca tttcaatcag tgccgttggg agcatgaagg aatccattga accaggccat
    50941 gtggtgttgc ccgaccagtt tatcgatctc acgaagcggc ggatctcgac gttttttgac
    51001 gaagggatcg tggcgcatgt cgggttcagc gagccggtgt gctcttcctt agccgatacg
    51061 ctagaggagg cgggacgatc cgcgggcgcg acgcttcatc gcggcggcac ctatgtgtgc
    51121 atggaaggac cgcagttttc gaccaaagcg gaatcccgcc tgtatcgaca atggggcgtg
    51181 gatgtgatcg ggatgaccaa catgcctgaa gccaaactcg cccgcgaggc cgaactgtgt
    51241 tacgccacga tggcgctggc caccgactac gattgttggc atgagacgga agaagcagtg
    51301 acggttgggg ccatcctggc caccttgcac cggaatgtgg ccctggctaa ggaaatattg
    51361 aagacggcgg tgccgaagtt ggatcccgac cgggcctgtg cctgtcggca agcgcttgcc
    51421 acggcactga ttactgcccc ggacaaaatg tctgcttcgg caaaacgacg gttgaacctc
    51481 ttgatcgctc cctatgtgcg gacgcggaaa ggaaaacggt gaggcatggg taaattgctg
    51541 gtagtggggt cggtggcgct ggataccgtg aaaactcctt tcggcgaggt ggccgaggta
    51601 cttggcggat ccgcgacgta tttttcgacg gcggccagtt atttcacctc ggtcgatctg
    51661 atcgcggtgg tcggcgatga ctttcctgag caacatgtcg cgttcttaaa aagccgaaaa
    51721 attgatttgg cgggattgga acggcggcca ggcgcgacgt ttcgctggaa gggcgcctat
    51781 tcgcatcaac tcaacgaggc gcagacgctc gacaccaagc tgaacgtctt tgagacgttc
    51841 cggccgaaga tcccggccca gtacagctcg cccgatgtgc tctttctggg caacatcgac
    51901 ccgaacctgc aactcgacgt gctgcagcag gtgaagcggc ccgcgttggt cgcctgcgac
    51961 acgatgaatt tctggatcaa cggcaaacgt gatgcgctct ggaacgtcct gcaacacatc
    52021 gacattctga tcatcaacga cggcgaggca cgggccttgg gtgaggatac caacctcgtg
    52081 aaggtcgcca aaaagatcct cgcgcgggga ccgaaacatc tgatcgtgaa acgcggcgaa
    52141 tatggtgtgc tgatgttcaa cgacaagcag gtgttcgggg cgccggcgtt tccgctggat
    52201 gatgtgcgcg atcccaccgg cgccggcgat acctttgccg gaggcttcct gggctatctg
    52261 gcggcgacgg gaaaccgttc acccgaagcc atgcggcagg ccattatttt cgggagtgtg
    52321 atggcctcat ttacagtaga agcctttagc cttgaccgat tgaggatcct agattacaaa
    52381 gagattgagg cgcgctttaa agaattcaag cggctcacgc attttgagga tatcggcgca
    52441 tgatcgtggt tgatcgacag gaaactcgga gctgcagggc cagcggtatg gtggggtcat
    52501 cctgcctcgt gttcctgctg gggctgtctg tcttgggcgg gtgtgccaac gaagagaacc
    52561 tgcgcaagtc caaaggattt taccaggagg gcgtggcccg gctgagttct gatcagcagc
    52621 aggcgtatgt gtcgtttcaa aaagcggtga agctgaatcc tgacaataag gaagcgcact
    52681 acggactcgg gcacatctat gcctcgcaag gacgactcaa gctggctgaa gaatccttcc
    52741 gtgaggcgat tcggatcgac ggcgactatg ccgaagccaa cacgtacctc gggcaggtct
    52801 tggccagtca agatcgatgg aaagatgcca tcgcggcgta ccggcaggcg ctcagcaatc
    52861 cgctctatcc cacacctgac ctggcgcggt ttcagctggg gaaagccttg atgcatgaag
    52921 gtgatctgca gggcgcgatg gaggtgttgg aggatgcgac gaccgtgacg ccgcccaatg
    52981 tgccgcccgc gatgctgcag ttggagctgg cgcgtgtgta ccacaagctt ggattcgacg
    53041 cgcgggcgcg tgaggctctg tcgaaagtgt cgacgttgga taaaggtggc gagcaggcgg
    53101 ctgcggcgca ggaactcctc ggtaagctca aaccataggc ggatatcatg gaatcagtgg
    53161 gtgaattttt tcggcaggtt cgcgagacca aagggctgac ggtggatgag gtggcctcga
    53221 agacccgaat ccgcacagac tttgtcaaag cgctcgaaga gggaaatttc gccaaattgc
    53281 cagaccaggt ttttgcgcgc ggatttgtcc ggtcttatgc gcggtcgctc ggattggatg
    53341 aggaagatgc cattcatcga ttcgtgcagt ctgccggcgc cttttacgaa aagcagggtg
    53401 agcgcgaacg cttgcggcaa cgacaggtcg aagaggagcg ccgtcgccgt gccaaccgca
    53461 aggcggtggg ggtggccatc gctgtcgcga ttgcgacgct ggtgttcctg ttgagccgtg
    53521 agcagacctc tacgctggtg agacgctccg gaccggatgc accaaccgtc agtaagaagg
    53581 tggccccatt tgccaaggat gcgaaggagg cgggttcccg ccctgcggcg gagctgccgc
    53641 ctcccattgc tcctccgctc aaaccggctg agccggcggt tgttcctgcg aagcctgccc
    53701 aagacaaacc ggctccggtt gcgcaagctc cagcgcccgt tgccgtggcc aaagcgccgt
    53761 tgccgtcgga tacggtcgtg actcagccct ctgcctcaat gggatcagac ggtcctctgg
    53821 caggattgtc ggtggatggt cccgtcgggt cggatggtcc gctcgtcctc gaccttgcgg
    53881 ctacggaatt gagctgggtg gtcgtccaag ttgattcggg gagccaccag gaagcgctgc
    53941 tgcgcccggg tgaaaaggct cagtggaagg cccaggatca gtttacggtg acgctcggca
    54001 atgcgggagg cgtccgcgcc gaactcaacg gcaaaccgca aaagccgttc ggccccagcg
    54061 gcaaggttgt ccgggacgtc gtgatcaaac ggtgacggtg tcgtctcact agctcgacgc
    54121 tccccgggtc gtcaggcgtt tcgtgcattc ctattctcac cgcctgctca ctctctccaa
    54181 tccatatgtc gtgagggtgt ctatggccct cctgctcgca ctcgaatgag gcgaggagga
    54241 ggcgtctgag gcgcaaaggt gtcacggctg gatggctgta cgtacttgcc gctgatctga
    54301 atagtctaaa tgtggtgaaa tcccgcgatt tttgctgttc tgccgctggc acagcccttg
    54361 attctccttc aagtcagcat ggatgaaaca gacaaggctg aggcgggcca ttcgcttgac
    54421 aagacagaaa acgcgttgct atagttcgcc tgctttatcg gctttgtcat cagtttttgc
    54481 catgctgatt cggaccgaga gttattgttc acgcggtggg ttcaacacaa aggaggagtt
    54541 ctgatggggt atttttctaa gtttgtcgga gtgtgtgcgg ccgtcacctt gttgtccgtg
    54601 gcggtggtgg gcgcagaaga aaaggatccg ttgaagcccc gtgttgcgcc ggatcagatg
    54661 gcggacgcga aggcgatgaa gaacccggtt gcttcgactc cggagagcat tgccaagggc
    54721 aaggcattgt atgagggcaa gggcacctgc ttcaattgcc atggcaagga aggcaagggc
    54781 gatggtcctg ccggcgcgat cctgaatccg agcccgcgta acttcaccaa ctgcaagttc
    54841 cacaagaagc ggaaagacgg cgaactgttc tgggtgatca agaacggcag cccgggcacc
    54901 ggcatggttt cgttggtccc tgcggcaatc accgaagaag aagcctggac gatcatcaat
    54961 tatgagcgca gcttctgcaa gggtggagac gaatagtcac gattctcggc cgataggctg
    55021 aatcggaggg gcggcgaggg tatcctcgcc gccccttttc ttttgtatcg cgctgacgaa
    55081 tgtcggcgca atcccgtgtg agtcccgagt aacgtgtgac aggaaggaat gccccgtcta
    55141 cctcatcccg gctttctgcc gtgacggata aagggcgaaa tgcaccgcat tttacggttg
    55201 gtcagagggg aacgtcatcg ttggtttggt gagcacggtg tcgagaccct tctccgatcc
    55261 acgcagtgtc gggaggatct gcggttgctg ttcctgtttg aaggtactcc gcgctgcgcc
    55321 cggtactttg cgattgatgt aagcggttac ctccgcaatc gtgacctcgc cattccgatt
    55381 gagatcggct tcgccccgaa gacctcgcag gagatagtac gtaaacaatc cgtgatgcca
    55441 cgtttcggat tccaagctct tgccgaagcc ggtcgtcgca atcatgtgca gtaccgatcc
    55501 tgatgtcccg agccatttcg gagccgctgc tttcgtgcgc ccgtccgctc cattcttgag
    55561 gactgtcccg tcaaagacga agagcacttg cttggctttc aatcttccca gcgccgcttc
    55621 caattccttg agtggataca ggcgagtggg tgatcccatc gagccgtcat aggggatcag
    55681 gaaggtatcg ccggcggcgt tcaccatcgc ttgccccgca aagtagacga ccaccagcga
    55741 ctctttggtc gtttttgcag gaagccaatc gagcagggcc tcttcgatgt cggggcgaag
    55801 ggcgctccag tcccgcagga ggcgaacgtt gttttgaggt aaaccgccga gggtctgaaa
    55861 ataggccgcg accgtctctg catcgagcga ggcatatttt cgcgccgcga tgtcctgttc
    55921 gcgataactg ctgagtccga tggcaaggag atagtcgcct ggacgctgtg ctcctgacgt
    55981 agcggcagga atgtgatcca cgtcctcgag gagcgcggct gtcggttgaa aggagagcgg
    56041 taatgtttgt ggtggagcgg ctgcttgtcc tgaggcgtta agcagcgcaa cctgtaactc
    56101 cgcccgttga agcgacgggg attgaggcat cgtcgcaaca aattccagcg atttggaacc
    56161 ggccgccggg atggtcccgg tggccagcgt tgtggcaggg aactgcgtaa ggaccgtctg
    56221 cgggcctgtc agtttgacgg tactgtcctg caccaactgc gtgcccgtat tgaccacgtc
    56281 gactcgcacg cggattcgtt caccactttc gatgatcaga ttgccgttct catccaacaa
    56341 cgtggctttg aacgaaagcg gctgaaccgg cgttgcagca ggagcggctg gaacggcggt
    56401 cgccttcgag cttggctcgg taggcgtggc agtcgtggca gccgcaggcg gtggtgtcgc
    56461 cggggccagg agcacccgtg cctcttgcat gaactgcgtg gccagcatgg tagacgtatc
    56521 ctgcaacagt ggatcgacaa tgtaatcgca gcgctgttgc gtcagttcga gttgcaaacg
    56581 ttctttccgg gtggcttgga gcggccgctc accgagcggc gcgccggacg catcgaaaaa
    56641 ttccgccagg gcatccaggc tgagttccgc aggggcacga tcatagaggg cgtccgtttg
    56701 cagcttgaag cgtggatcca gcatacgcac gcgtatcgtc acgtcaggtt ttcccccggc
    56761 cgcctggctt cccggaatcg tgacggtgcg aaacgtttga tgtgccgcct ggatcaatgt
    56821 gtcttcgacg gttggcccga tcgagaaggc gtggatgcgg ctgcagttat cgatgtactc
    56881 cgtcttggct ttggagagag acggatcgat ctccatgtga acggtgaggg ggacaggagg
    56941 gcctagatct ggcaacgctt gacgggaaaa tcgcgatttg atggactcgc agccgacgga
    57001 agccagcagg gagagtaggg caattccaac aaggactacg cggtttgtct tcaagcaggc
    57061 ctcctcgacg gcagaataag aatgggaaac ggtacaggaa tgaggggggc attgcaacca
    57121 agcgctgggc cggcttgcga ccctcaccgg cctcgtttat cctaccgccg cgcctcgcct
    57181 aaacggcgca ttgatcggcg cgggagtcgc gttgctcctt gctcctcagt caggcaatga
    57241 aaaagacaaa aggacgttga cgtatcgtgg tggtgttagg tttgtccaat atgcgagatg
    57301 ccgcggccgc cctcgttgct gacgggcgga tcgtggctgc cgcagaggaa gaacgcttcg
    57361 tccgccaaaa gcacgtgacg gccctgccag tgcatgcgat gcagtattgc ctgcgcgaag
    57421 ccggaatggc tttgcgcgac gtcgacgcca tcgcggtgcc ctggaagtat tggcaggtgg
    57481 ggcggcgtct caggcttgcg gtgacggcga tgctgcgctc gacgcaactg ttccgtgtga
    57541 aggggacacg gtcccttgag cgggcgagtc aagaatggaa ggaactcttc cggcttcagc
    57601 aggaactatc cacgcgggtt gaaccgggtg ccggccggcc ggtgtttctc gaccaccatc
    57661 tctgccatgc agccagctca tttctcgtct cgccatttga acgggcggcg attctggtgg
    57721 tcgacggagc atcagaatcg gatacgacct tgctggctac cggtgaaggc aatcagatca
    57781 gcgtactgga gcgcacgccg ttgcctcact cgctggggca attctatgcg gcgatgacgg
    57841 cgttcctggg gtttcgcccg gatcaggatg aatacatcgt gatgggtctc gcttcgtccg
    57901 gcgagccgtc gttcgccccc acgctgagtc gcgagatttt gcgagtgctc cccgatggac
    57961 gattcgaact gaatacccgc ctgctggatt tccacctcgc cagggcgggc catttcgtgc
    58021 gggaattcat ccggctgttc ggtcctccgc ggcggccgca agacgagatc acgcagcgcc
    58081 accgcgatct tgcggccagc gcgcaattcg tgttggaaga aaccctgttg catctggggc
    58141 gtcgtctgcg ttcgctcact caggcgacat cactttgcct cgcaggtggg gtagcctaca
    58201 actgtgtcgc caacggtcgc ctgcgggccg aactcgggtt tgaccaggtg tacgtgccgc
    58261 cggctgccgg agattccggg gcggcgctcg gcgcggccct ctggtggacg gcgcggcgag
    58321 gagcggcgac gtcgaagacc gttatgccgg atgcctatct cggtccgcag tatgacgaat
    58381 ccgcctgccg tgccgccttg tcgaagacag gactggttgc cgaacatctg gcagatgacc
    58441 gattgtacga acgggtggcc tcggagttgg cccatgggcg attggtgttc tggtatcaag
    58501 ggcggatgga gtggggcccg cgcgcattag gaaatcgcag tttgctggcg gatcctcgac
    58561 gggaagacat gcgtgaattg atcaatagca aggtgaaatg tcgcgaggct tttcgaccat
    58621 tcgcaccctc cgtccttgcc gaacacgcgg aggagttttt ccatgtgcct gccccctcgg
    58681 ccttcatgca gtttacggtt cgagtgaaag catcagcgaa ggggctcctg ccggccgtga
    58741 cgcatgtcga tggtaccgct cgtgtgcaaa ccgtgacgcg tgaagccaat ccccgcttct
    58801 atgatctgct ggcgaccttt ggcagattga cgggtgtgcc cgtgctgctg aacacgtcgt
    58861 tcaatgtgca ggaaccgatt gtgtgcagtc ccgacgaagc agtgcgttgt tttcagcgta
    58921 cccaggtgga ctggttggtt ctcggcaatc ttctggtggg acggccgtca ccgatgccgc
    58981 cgcagcatca tgaaggctga gccggttcgc ggttcctttc ctcttcctgc cctcatgctc
    59041 atactggatg tgttgctggg cgcgtttgtc ttgaatctgg tgctccaacc cttagtggaa
    59101 ccggatctag gatggcatct ccgggccgga ctggatttga tcgcgcaagg gtggcgtttg
    59161 ccggataccg acccctattc ccatacgatg ccggattggc attgggtgga gcatgcctgg
    59221 ttgaccgatg ggctgttggg attaatctat cggatgcttg aaccatctgg cggattgggg
    59281 ctgattgcgt tttttggagg agtcacagcg ctggcctggt ggatcgcggc aggagtagct
    59341 tccgtccatc gcacgtgccg gttagcggcg atggtcgcga gcctctgggt ggccttaccc
    59401 tttctcggcg cgcgcacgca gttgatcagc ctccttggcg tggcagttgt gctcacgata
    59461 tggaatcgca tgcagcgggg acagccgcaa tgggtctggg ctctgccgcc tctgtttctc
    59521 ttgtgggcca atcttcacgg cggcttcacg gcgggtctgt ttttaatcac cgtgttgttg
    59581 tcgctctcat tcctcgtgaa aatctgcgtc gagtggaagg tcgttcaggc accgcgtgat
    59641 gaaccggtgc tcagctggga tgttatccgg ctgtgtgctt tcgccattct tgtctctttt
    59701 gctgtgacct gcctgaatcc ctatggctgg cgtctctatg tggagattta cgaatcattg
    59761 acggatcgct tcatgatcga caccctgcgg gagtggcagc cggtgtcgtt tcacgggtgg
    59821 gccggacggg cctatgggtt ctatctcctt ggatttatcg ggttgatggc cggatggtat
    59881 cggcgagtgg agccggtgcg gtgggtcctg ctggcgctga tgctgggatt gtctttgctg
    59941 cattggcgga atgtcactct gtttttgatc gtgagtctcc cgttggcggc agagttaggt
    60001 gcggctgcgg cagattcctt cctgcgtcgc gttcctgtgc tgcaagccca cgctgccggc
    60061 actgtccttg tggtatccat tatggcggcg acggcgctgt atgccttggg aactgagcac
    60121 ctggagcatg tgtggcaatc aggccgtact cccgatcggt attttgagcg cacggagtat
    60181 cccatcgaag cggtgcggtg gattcgggct caccgggatg aaacggggac gaggctctat
    60241 aacgactata gctacggcgg gtttctgcta tggcaacttc caggcgagcg aatcttcatc
    60301 gacggacgca tgcctgcctg gcggatcaag gacagatgga tctttcacga ttacgttgaa
    60361 ctcaatcgcg aagattcgcc gcaattggcc ttgctggatc gatacggggt ggattgggcg
    60421 atgatcccgc gtggcagcgc gttggcagtg gctctggagg cgcatcctgc ctggaagaat
    60481 gtttatgccg atgccaaggt ggtgattgtg cgccggcagt cgtgaccgca gggtgcgtcg
    60541 tttaggaatg cgtcggttcg actgtaacga ggtccggttc gacggcgctg gatgccgggc
    60601 gtgattgggg ctggtaaagg ccttgggcct cgcgcttacg gatggccaga agttcccgca
    60661 gcatcaagga gccgtcgcgc caagggcgaa ctttcgagcc cggctgatcg gtccagttga
    60721 tagggacttc cgcgatgcgg tagtgccgtt ggcgggcgat gtgcagtaat tccaggtcga
    60781 atgcgtagcc gtcgacacag gatacggcaa acaaatcctg agccaccgat tgtcggaaca
    60841 acttaaaccc gcattgcgtg tcggcaatat gcggcacgcc cagccgttgc accacatggt
    60901 tgaagaggcc ccccagcagg ctccggtgcc atcgggcatg aaccgtaaag gccgggtcgt
    60961 gggaggccag ggcccgtgat ccgatcgcaa ggtcagcccc ggccgcgagc gccgcttcca
    61021 atcgtccgag ttcttcaatg ggagtggctc catccgcgtc ggcaaatagc tggatggttc
    61081 cgcgcgcagc ctgcatgccc cgtcgaaccg ccgcgccttt gcccatgttg caggtcaatt
    61141 gaatcagccg cagatgagga cagtggtgtg ccgccatcct gacggcgtga gcggtgttgt
    61201 cttgactccc gtcgtccacc accagaattt catagaattg cccgcgctgg tccagatggg
    61261 caatcacacg ttgcaaaaac agcgggagcc ggcgcgcctc attgtaggcg ggaatgatga
    61321 ctgaaaggga tggtgtctgg gtcgagcaca gagtcacgtt ggaacgcccc cggtgggagt
    61381 gtgacgcgac cgcttcgtcc ggcgacgaag ggtgcaccac ggtagctgag ccatgatcga
    61441 aatgcaagcc atggttggca catcttgaca gaggcagagc gcgggagtga tcgtatctca
    61501 gatacggcgg tatccttggc gtcgatccgg tccggaggat gtcatgaggc gcagcgcgaa
    61561 ctgcttggcg tcgctcgtcg gcatggtgat cgtgacgagt tgtgccgtcg ccagtccgcc
    61621 cagtcgcctc gctcagtatg tcggatccgt cgtgccggaa gaggcggcga cggcgacgct
    61681 tcccacttcg cgtccgctcc atgccgcgct ggtcgtggtc gcggatcagt cggctccgga
    61741 ttcagcgccc gttttgccag acgaagccct ggcgcgggtg ggagcgctcc tcacggggga
    61801 gatcaaccgt gggtatcccg tcgctgttga ccgagtcgtc aagcttgatg ggatgtcacc
    61861 cgggggaaca gcgccgagct ggcctgaggt cgccaggcag caaggggttc agtatctgct
    61921 ggtgacggtt ctgtcgagca ccgagcagga atatccgatt tctctctttt tggggtggac
    61981 gacccaccaa cagcctggat ttcggcgcga taactggtct ctcgccgaag cggcgttgat
    62041 cgacggatcg accgggagga cggtgctgca ggcggaagga cgagccatgg ccacgttgga
    62101 ccgcccgacg gcgccgggaa tttcgcaatg gtatcccgtc atttatctgc ggccgcaaga
    62161 cccggagcga cgcatttggc cgcccaccta tgaaggggct ccgctcacac tccgtctggt
    62221 ctcgatggaa caggcggcga agcggttggc cggcaatctg cagcgagcct gggtagaaca
    62281 gcgggatctt gaactcgctg cgcgctaagg ctctgagccg tcggtcggag gtgacgcagg
    62341 ctcggtccag acgatgatgg catcaggttc ggagccgcgt tttcctcttt gaaggcgatt
    62401 ctgtgattag tgtagaatga aacgttcaac gttggcgcgg aggatggtcc ctcaatctca
    62461 ggtttccaga ggagtacaca tggtgtatcg acacgagcgc aggagagtgc ggtccgtact
    62521 cgcagtaacc gcgatggttg ctgccacatt cttatccggt ggattaccgc cccgggccgg
    62581 tcaggccgag gctgctggtg ttcccccggc attcgctcaa gggttttctg aaatcgtaaa
    62641 agctgtcacc cctgcggtgg tgaacattgc ggtgaccggt ggtggcgagg gacgccggga
    62701 aggtcgtcgt cagcttcccc caggcggtcc cttcggcggt ccgcctcctg gtcccggtgg
    62761 tcctggtgat gaacctcctg gcatggagcc tcccggtggc cctccgggcg gtggtcccca
    62821 tcgtcccgag caaagcgcag ggtccggcgt gatcctcgat ccgaacgggt atatcgtgac
    62881 caataaccac gtggtcgaag gcgccaccca aatcacggtg accctctcgg atcgccgcga
    62941 attccccgcc aaaattatcg gaaccgatcc caagaccgat ctggcgatta tcaagatcga
    63001 agccaaagat ctcagctcga tgaagtgggc ggattacgat gagctgcagg ttggcgatgt
    63061 ggttctggcc gtgggtagtc cgttcgggct cagttcaacc gtcaccctcg gcattatcag
    63121 cgcgttgggt cgggggaacg tgggcatcgc cgactacgaa gactttattc aaaccgatgc
    63181 ggccatcaac cccggcaatt cgggcggtgc gctggtcaac atgcaaggca agttgatcgg
    63241 catcaatacg gccatcttct cccgcaccgg cggatcggaa ggcatcgggt tcgccattcc
    63301 cagcagcatt gcgaccgata tcgtcgatag tttgacgaag accggcaaag tggtgcgtgg
    63361 atggatgggt gtggccattc aggaaattac tccggccttg gccaagtcct tcaagttgcc
    63421 tgagcagcgc aagggcgtct tgatcagtga cgtgaacgag aatgggccgt cccatacggc
    63481 aggcatgcgc cgtggtgacg tggtcatttc cttcaatggc aaggaagtgc agagcgtcag
    63541 ccaattgcgc aacctggtcg cgcgcatggg tgtcggcaaa gatgccgaca ttaagatttt
    63601 gcgcgaaggc aaggagcagg tgttgaaggt gaaggtggct gagcgtccgt cggatgaggt
    63661 gttagcgaag cgtgagccgg ctccctcggc ggctcctgcc gagacggtga agccaccgga
    63721 caacgtcctg gcggccttgc gcgtccaatt gctggatgcg gcgatgcaga gccagctgaa
    63781 catcccggcc aagactaccg gtgtcgtggt gagttccgtc gaggcgggca gtgccgctga
    63841 gtcggcggga ttgcagcgtg gcgacgtcat tcaggaagtc aaccacgaag tcgtgaagag
    63901 ccttgaggat taccaaaagg cgtcagccaa ggtgaaaaag gacgaaatgg tcgttcttct
    63961 gctgagtcgg cagggaaaca atctgttcgt tgccgtcaat ccgaagtaag acatggatga
    64021 gggaacgatc cgtcgagtga gcggggaatt tctcccgtac tcactcgacg gagagtcctg
    64081 cctcaagttt gtgaacaggc atggctgacg ggttcaagtt tcaagagtgg ctgcaagaca
    64141 cagtctttaa gccgcttgaa gataaaaaaa tgccggtcat ggagcacttg gttgagctcc
    64201 aggtccggct gacccgtgcg gtcattgtga cggcgatcat ttttgtcggc accttcttct
    64261 acgccgacac gctggtgaag tggatcagaa tcccgctcca gaacatgttc gtgccggggt
    64321 ccctttcctg gatccccacg gatcttccca ccgtcccgtt cgtctttctc gcgccggctg
    64381 aagccttatg gcaaaacgta aaggtcgcag gtttgtttgc gctggtgttg ggcactcctt
    64441 acatcctcgt tgagttctgg catttcgtgg tgccggggtt gcacgcgcag gaacggagat
    64501 tcgtcgggcc ctttgtgatc ctcagcacgc tggcctttta tctgggcctg ttgttttcgt
    64561 tctttttcgt cctgcccttt gctttgaact ttctcatctc gtatggcgtg aatgccggat
    64621 ttattccgca actctcgatt gcgcaatatg tcggattcgc cttatggttc ctgctggtct
    64681 tcggtctcat ctttgaagtg ccgctggtga ttaccctgct ggctaagcta ggctgggtgg
    64741 atgctccatt gctgaaacgt tatcgcaagt gggccttcct gacggcgttt attttcgccg
    64801 cgattctcac cccgacaccc gatccgttca accagtgcct catggcactg cccatgtaca
    64861 ttttttatga agtcggcatc gtgagcgccg gcatcttcaa caaaaagaag gccgacgcgc
    64921 cggcggaacc actcgtgccg gcagtggtgg cggcgggacc ggggctcgcg aaagcgggac
    64981 caaccattcc gtctggcggt ggagacgatg agtatgtcgg cgtgccgagc ggcggccggc
    65041 gaggatgagc aagaccggag tagagcaaca gggcgaaagc gcgctttcgt cttacgcagt
    65101 atgatgttcg ttgtacgagg agttgaggaa tgccacgcga gttaaatgtt atttcacagc
    65161 ccggttccag cagcggcgga gatgcctgca cttatatgtg ggcctgcgcc atttgcgacg
    65221 aaaatgagac ttgtcaaaag gacaaggaag gccacagccg ctggttggtc gcaaagcgca
    65281 tggaacggat cgaatataaa gtgcttgtca tgagtaataa aggcggcgtg ggcaagagca
    65341 cctgcaccac gaaccttgct gtcagtcttg cgctgaaggg ctggcatgtc ggtatttgcg
    65401 acatggatat ccatggcccc aatattccga agatggtcgg ggcggaggga caaaagctga
    65461 agatcagcac ttcgggcggg atcatcccgt ttcaggccta caacatgaag attgcctcga
    65521 tgtccttcct gttgcagaac tctgacgatc ccatcatctg gcgcgatgcg tataaatatg
    65581 aattcatcaa ccagttgctg ggaggcgtgg agtggcagga tctgaatttc ctgctgatcg
    65641 atctgcctcc tggaacgggc aatgagtccg tgacgaccat cgatctcctc ggtggggtca
    65701 gcggagcggt cattattacg acacctcaag aagtggcgtt gctcgattcc cggaaatccg
    65761 tgacgttctg caaggacagc gaagtgccga ttatcggcat cgtggagaac atgagcgggt
    65821 tggaatgccc ccactgccac aaggaagtga atgtcttccg caagggcgga ggtgaggcct
    65881 cggccgcgga tatgggtgtt ccgttcttgg gacggattcc gctggatccc gacgtggtga
    65941 cgcaaagtga tgcgggcgag cccttcgcac tgttcaactc cgatgcggca acggctcagg
    66001 cctatcacga tatcgccaat caggtggaag ctttctgcaa gaaaagcggg tcgctcataa
    66061 agccggcgcc gcgacagcaa cattgacggg acggtgacct gtgaaatctg ccgatgcgac
    66121 agtcggattg acgccgcttc atgaagccca gcgcattgtg ctggacgccg cggctccctt
    66181 agggctcgag aaggtctcca ttatggacac cctgggccgg gtgctcggcg aagacatcat
    66241 cgctacgcgc tcgaatccgc cgtgggacaa ttccgcgatg gacgggtttg ccgtacgcgc
    66301 tgaggacatt gcgcaggaac acgcgatttc taagcctgtc acgctaaccg tcattgagga
    66361 tgtgccggca gggaagatgc ccaccaagtc tgtgggaaag gggcaagcga tcagaatcat
    66421 gacgggcgcc cctattcccg ccggggctga taccgtcatc aaggtcgagg atactgaaca
    66481 tacgcccgat tccgtgcgtg tgttcaaagc ggaaccgcgc ggctcaaaca tccggccgca
    66541 gggtgaggat gtgcaaaagg gcgactgcat cattccgaag ggcacccaga ttcgatccgg
    66601 cgaagcgggt atgttggcta ttctcgcgaa gtcctttgtg ctggtctatc agcgtccgcg
    66661 cgtcgccatc ttgtcgacgg gcgatgaact cgccgatctt gacgaacgct tcagcgaaga
    66721 aaaaatcatc aattcgaaca gttacggtat tgctgcggcg gtgcaagaag ccggcggcgt
    66781 gccgatcctg ctgggcattg ctcgggatac gcctgcggcc ttaacggaaa aaatttccca
    66841 cggattaaac gcggacattc tcgtgctatc aggcggcgtg tcgatggggg attacgactt
    66901 cacgaaagca gtattccggg acctgggtgc cgagatgaat ttctggaagt tggccatccg
    66961 gccaggccag ccgctcgcct tcggcaaaat tcagggaaag ctggcgttcg gactgccggg
    67021 aaacccggtc tcttcgatgg tgacctttga gcaactggtt cgtccggcca tgttgaaact
    67081 gtccggcgcg cgaagctatg gacgtccgtt gctcgaggcg gtctttcagg agaagttttc
    67141 caagcgaacc gatcggcggc attttctgcg cggggtgttg tggcgggaag atggagtatt
    67201 caaggtcagg acgaccggcg atcagggctc gggcattttg acctccatgg tcaaagcgaa
    67261 ctgtttgatc gatattccgg tcgaagtaga gcgcctcaat cccggtgatc cggtaaccgt
    67321 gcagcttctc agcgggtcgg tatggctgga ccaggccgct ccggctgctt ctgccgctca
    67381 ccgcccctcc tgctgctgac gccgcgaaag gaacgttgtc atgtccgtgc ccattctgtc
    67441 ttttgtcggc cggtccaata gcggcaagac cacgttgatc gaacgcgtga ttcccgagct
    67501 tgtgcgtgcc ggctataagg tggcgacggt caaacatgcc ggtcacgggt tcgatttgga
    67561 taccgaggga aaggacagct ggcggcacaa gcaagccggt gcgagcagtg tcgtgattat
    67621 ttccaagagc agtctggcca tgttcgccga tgtctcggac catatgaacg tcgaggacgt
    67681 tcgtgagcga tacctcgatc cctcgtatga tctgatcctg gccgaaggat ggcggagcga
    67741 aggctatccg aagatcgtcg tcgtgcgcga ccagatcggt gaggtgccgg tttcgcaaga
    67801 cggcctccta gcggtcgtgt ccaacaaacc cgtcgaaacc tccgtcccgc tgctggatcc
    67861 tgatgatgtg gcggcggtgg cggcattgat catccggcat ttccccaaat cccaacggga
    67921 tgatgcgtag tactcccgcc attgtgttcg gggcgattct ggccggcgca gtggctttcg
    67981 ggggagttgc ggttccgctg acgaaccatc cgaaattctg cgccagctgc cataccatcc
    68041 aacccgccta tgagggctgg ttgcaatcat cccataagga agtggaatgc gtggcctgtc
    68101 atgtcaggcc cggtgtggtc ggctggttgc atgataaggc ctggcatggg acgagggacg
    68161 tggcgatcta tctgttcggc aaaccgacgg agcctcacaa tctgcaagct caggtggatt
    68221 ccgccgtctg cctcggctgc caccgcaata tcctgcgggt gtcggaaatc gcgcctcgtg
    68281 atttgccgtc tcccgtcaag gatgtggggc tgatcatgag ccatcggaaa catatggcgg
    68341 cgtttgatca gcgtgcgcaa ggcgaagggt gtaccacctg ccacgcggca gtggtgcatg
    68401 aacggccgat caaaggatat ccaatcgtca ttccccgagg ccatgtgtca gctgatagcc
    68461 gtccgtggaa ttccacacat cctgagggct cggtgttgca caaacgggca caggcggatt
    68521 gctttcgatg ccacgataac aaaaccgaat atgaaggccg ggtgctgagc cgaaagtgcg
    68581 aaacgtgcca tctgcctgaa aaactctcgg aattcctgtc gttttagccc acggaaccag
    68641 ctgcccatgt catcgccggt ggtcgaagta cgtaatctca gtaaacgatt cggtggattc
    68701 accgcggtcg acgggatttc tttcgagatc ggccgagggg aaatcctcgg cttattggga
    68761 cccaatggcg ccggcaaaac gacgacgttt caaatgatgt tagggttagt cacgcccaca
    68821 gccggctcga tccgcatgtt cggtctcgac ttgcagtcga atcgggaaac gattcttcag
    68881 caggtcaatt tttcctccac ctacatttca ttgccgttct ccctgacggt tgaggaaaat
    68941 cttcgagtcg tcgcccgatt gtacggcatg tccgatatcg cggcccgtgt ggatgatatg
    69001 gtaaagaaac tggagatggg cgactggcgg cacaagctga cgcgcaaact gtcgtcaggg
    69061 cagatgacgc gtttgacgtt ggcgaaagct ctcctgacgg aacccaaagt gctgtttttg
    69121 gatgaaccga cggcgagtct ggatccggat atcgcgcaca agattcgcgc gatcctgttg
    69181 gaggaacggc aggcgaccgg actcagtatt ctttacacct cgcacaatat gcgggaaatg
    69241 gaagagatgt cggatcgcat tatttttctg cagcggggaa agattgtggc cgaaggcacc
    69301 gccaaagaca tcgtggctcg atttggtcag gcggacctgg aggatgtgtt tcttaaactc
    69361 gcgcgcgagt agcaggcccg cgcgggatcg tgtgatgtgc aaggggagca ggcagcgcca
    69421 tggttgatgc aggaatatcg tttactgtgg gggtactcat cggcggcctg agcctcatgg
    69481 tgggatgggg cttgttgtgg ttcgtcgtgg gcgggatcgg cttggcacgg cagaccagcg
    69541 gatggccgat ttttatcagc agtatcagca gcaccgcgat tgcgggcggc gtcatggctg
    69601 tggtgttctg ggctgtcgat gccgcacgac tgagcagccc ggcgttgcac gtcggtctgg
    69661 cagggattcc actggccttg cttgcggcgt cagcctttcg cttggaagac ggccggcgaa
    69721 ttggtccggc gtttgtgcaa gggagccgct tgatgatgca ggaactactc ggcgggcatc
    69781 aggaagaggg cggatgcggc cattgtcatg aacagccggt tgaagagcat ccctgtcacc
    69841 agaagccgtg cttcgaacag tcctgccacg agaagccttg cgaagggaag ctgtagccgg
    69901 cgatggccta ccaccgtatt gcggctttgg tcatccgtca cctgtatctc taccggcgca
    69961 gcctgccgcg ggtgatggag atcatctatt ggccgtttct cgatctggtg gtgtggggct
    70021 tcatcacggt ctatctggcg acgttccaag ggcagatgcc ggcggtcgtg acgtttctgc
    70081 tcggtgcgct gattctctgg gacgtgttgt ttcgatcaca gcagggcatt acgatttcct
    70141 ttcttgagga aatttgggcg cgcaacctga tgaatctgtt cgccagtccg ctgacgccct
    70201 ccgaatttct cgcggctacg atggtcatga gcctcttcaa ggtcactgcc gtgtcggtgg
    70261 tcatgtccct gtgcgcctgg gtgttttacg gctacaatgt ttttatcatc ggcctgtggc
    70321 tcatgccgtt cattgccaat ctcgtgctaa cgggatggat catcggtgtg ttcacgacgt
    70381 cgctgatcat gcggttcgga caggaggcgg aggtgctcgc ctggagcatg gtctttttgt
    70441 ttcagccgat ttcctgcgtg ttctatccca tggatgtcct gccggcctgg ctgcgaggcg
    70501 tggctgcgat caatccggcg gcacatgtgt tcgaaggtat gcgaggactt cttgcgaccc
    70561 atgcggctcc gttgaccaac ctcggaatgg cgagtggttt gaatctgctg tatctcggcg
    70621 cggtggtctt ctggtttcat catacgttca atgtctgtaa agagcgggga tcgttggtcc
    70681 gggttggcga ataattgatc gcgttcggcg tgcgcgggtt cgaggaggag acgcatgaag
    70741 gttgtagcag gacaagtcgg acaagcacgg tcagtagccg ggctggagaa tttttccttc
    70801 cacaagacgc cggtcctggt catcgagaac ttctggtcgg ccgacgaacg aggccagttt
    70861 cgagaagcga tggcgcgggc caattggaat caactgcaag aaatgtcgta cgtccggcaa
    70921 gacttcccga attccgggaa ctgggcgaag gctgaaatcg cccagccgca aggacggctg
    70981 ctcttgagcc gattggaatt gccctgcatt cagcagtata tcgaatcatt tcccaacatc
    71041 acccgccgtc atatggggtt cagctactat tcctatggag ccggtgattg tttgttgacg
    71101 catgacgaca cggatcaggg ccatccaacg agcggcaggc ctgcgcctcg tcgacgcatc
    71161 gcactcgtca gctactttca cgaggaatgg gattgtgact ggggcgggga gctgatgatt
    71221 tatgcgcctg gaggtgagtc aaaggccggg aaaccggatt tggccatcac acattgcatc
    71281 gcaccaaaac cgggatcgtt ggtcatgttc accgtgcctc gttttcatcg cgtgtgccgg
    71341 gtggatcaaa cagccgggga ccacaagcgc ctctcgatcg ctggatggtt tatgaccgaa
    71401 catgcctagc aggatgttga aaacgtctgc cagtctcgtt ctcgcaagac actgcaccct
    71461 caccgtctcg gcggcgttca caaacgtggt gcgcggtatt cagcggaacg gcctgtttga
    71521 ccattccacg ggttacacac caacatgccg cttcagtacg accatcaacc tgaccgccca
    71581 tctcgcaccg gttcaaaccg cgttcgccac tccagcagag gtctgatcac gcccggccat
    71641 cgtccgtgaa agtcacctcg cgccgaatct ccatcagcac tgtcatccac ccggaacccg
    71701 agagcccgcg gcgcatgcgc atgcaggcga aatggatcct ccgtcatcat gagggggctt
    71761 acggtcaaca ttccctcggc gagtaagttg cccggaatcc tcgcggtgga ggtataccgg
    71821 ccggcgggac ggggggttcg ttgccatgcc ggatcccgat cgtgcgcgat gaacacggtc
    71881 acgtcttctt cgttgtagag attaaagacc ggaacgaaga cttgccccgg tttaaggaca
    71941 tcgaattcca cctcgagatc aatcggccgc cggatatcga agcgatcttt gatcaaccct
    72001 gattctgttt gggcccgcac tgcccgcaga cggacaacct cattgcccgg ggcttgggac
    72061 agatccggcc attcggcaca gggctggatg tgcccggcat gcagatagtg attcacca
//
LOCUS       DAOQDS010000010        71831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_116_length_71831_cov_20.425086, whole genome shotgun sequence.
ACCESSION   DAOQDS010000010 DAOQDS010000000
VERSION     DAOQDS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 71831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 71831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71831
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116_length_71831_cov_20.425086"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PK589_03715"
     CDS             complement(<1..1061)
                     /locus_tag="PK589_03715"
                     /inference="COORDINATES: protein motif:HMM:NF039196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNJ18731.1"
                     /translation="MNTKLRSRSGKRQQSGVLYEEGHDKAVLNILQAVKKGDFSVRLP
                     AEWDGMAGKIADEINGLIELNAMFSEELKRVSHAVGKKGKLSERASVPEARGEWKTNI
                     EAVNGLIDDLVRPTTEMARVIGAVAKGDLTQSVALEAEGHPLRGEFLSTAKTVNSMVG
                     QLDAFAAEVTRVAREVGTEGKLGGQAVVPGVAGTWKDLTDNVNSMASNLTNQVRNIAE
                     VTIAVANGDLSKKITADVRGEILQLKDTINTMVDQLRSFAAEVTRVAREVGTDGKLGG
                     QAVVPGVAGTWKDLTDSVNSMASNLTNQVRNIADVTVAVANGDLSKKITVDVRGEILQ
                     LKDTINTMVDQLRSFAAEVT"
     gene            complement(1349..1735)
                     /locus_tag="PK589_03720"
     CDS             complement(1349..1735)
                     /locus_tag="PK589_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18732.1"
                     /translation="MINELNRPIQEPNVGRAVLGTSLGQSASYCGATAILSLHQPVIE
                     VSESDGNFNVYADFRPVLENTSMQVAVEFAQQGIILTGGTVERYLPIPTDAYIGHARV
                     TVTGGIARISIPTADAGHRWRSIVMW"
     gene            1943..3919
                     /locus_tag="PK589_03725"
     CDS             1943..3919
                     /locus_tag="PK589_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ18733.1"
                     /translation="MPSPDETSPSIPQPGFPLDFMLAHLAAIVECSDDAIMSKDLNGM
                     ITSWNRGAQHLFGYTACEMIGQPVLRLIPEDRQAEEPRILERLRRGERIDHYETVRKR
                     KDGTLIEISLSVSPIKDREGRIIGASKIARAISELKQGERNLAHLVEALPAAVYTTDA
                     DGRITRYNQAAVDLWGQRPSLETARWSGSWRQYWADGRPMPPEECPLAQSLALNRPVS
                     GLETIVERPDGTRLPVASHPTPLRDASGAPIGGVDILVDLTEQKRAERDMQNSAAELE
                     RRIVERTGELLLSQERLRALASDLTLTEQRERRHLATELHDYLAQLVVASRIRLAQVI
                     PTIHDATASAALTKIDGMLDQALTYTRSLVAELSPHILYQFGLSQSLVWLGEQMKQHG
                     LEVAVKTMAPSFTLPDDQAVLLFQSVRELLFNVIKHAKVDRAAITINVDEDRRELWIC
                     VEDEGAGFDVTDLSVAKNTRCKFGLLSIRERMELLGGECEVSSVPGSGTVAILRLPLA
                     SGALKQPFPLAADTAQTITPSIDASSRNINILLVDDHAMVRQGLRSILDAHDNLTVVG
                     EGADGQDAVVMARSLRPDVVIMDVNLPIIDGIEATRILTQEHPSIMVIGISVRNDPQV
                     HDAMTEAGAVAFLPKESAADQLYEVILRAFPVQA"
     gene            4127..7672
                     /locus_tag="PK589_03730"
     CDS             4127..7672
                     /locus_tag="PK589_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ18734.1"
                     /translation="MTSVLLTLQRLVPSAAGLAIGFPVFAFLCWAYNPTFLYNLSPSA
                     GTVKPSTMAAICFCGLSLWLQADEAAMTRARRRMAQGFAGLAGLLAAVTMLEYLLSLD
                     LGVDLWLASVPDLAVDSHPGRMAPATAFALFMLGLALIQLDSKHSGQDQASHYLALAG
                     GTIGAIALVGYLYGVQTLYKVGTSNTISIFSAALLLALGLGILSARPTRGFMATLTSD
                     ELGAVMLRRVLPFAIALPILAGWIRIAAQRSGRYDFNFGVALAVSAVMLIILAFLWLL
                     AGNLNRSDGQKKHILQILQQREARHRLALKAGRMGTFHQDLDRHTLVFSPELEHIFGL
                     PAMSFGSTFAAFQDLVHPEDRAHVLKTINYAIQHRSAYDLECRILRQDPPEEAWIAVT
                     GQVLPDSTGHPRQITGVMFDITERKHSVTALQQSEQQLRVITDALPGLIASVDCDERY
                     RFVNAGYEHQFGLSRDQIVGLSVKDLLKEDYSTVQPFIRRVLSGERISFETTLLDERG
                     EQTLLSTYVPDRNSEGMVAGFYALITNITERKRTESALRESEERFRHLFEQASDGIVT
                     ADMNGQYLDVNNRACQMLGYTREELFDLRVPDLLDPHDYPRLRAIKAELIQGHRHSGE
                     WKLRRKDGGTISVEISARLQPDRRWHAIVRDITERKRADAGLRESEAYFRMLADATPV
                     LVWKAGTDRLCSWLNRSWLEYTGRLLDDELGTGRTDSIHPDDRDEFNRVYNQHFSRQE
                     PFELEYRLRRQDGTYGWILDRGVPLLSAAGVFSGYLGAALDITDRKLAEEQLQQWTVE
                     LEKRVDERTQALVHSQARLRALVSDLSATEEQERRRLATELHDYLAQLLVVGRMKLSQ
                     ARPQVRNPKTEQLLSEADDMLTQSLNYTRSLVAELSPQILYQFGLPAALKWLAGQMKS
                     HGLSVSIQCGIDQLTLAEERAVILYQSVRELLFNVAKHAGTGEAMITLDQSPTNQVLI
                     TVADNGRGFNPAILVDTEGHHPGRFGLFNVQERIEAIGGTLSLKSSEGCGTTVTLIVP
                     LKPFPESAVESDLPVDGHVPDVLRTPSQRLRILLVDDHAMVRQGLRSVLESYTDLEVV
                     GEAGDGGTAISIASALKPDVVVMDINMPNIDGIEATRHILSEHPDVVVIGLSVQNERH
                     IEEAMLKAGAAVFVTKERAAGQLYEAIVSTVRTRR"
     gene            complement(7687..7854)
                     /locus_tag="PK589_03735"
     CDS             complement(7687..7854)
                     /locus_tag="PK589_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HNJ18735.1"
                     /translation="MLSWAVTFLVIGLIAGVLGVTGVAGTATHIAYVLFVIFLILAVI
                     GMVMGRRPPAV"
     gene            complement(8141..9205)
                     /locus_tag="PK589_03740"
     CDS             complement(8141..9205)
                     /locus_tag="PK589_03740"
                     /inference="COORDINATES: protein motif:HMM:NF018439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HNJ18736.1"
                     /translation="MALFLLGVMAGFAGMAMAASDEYIAGYAAGLLEHEFHLSGAVVQ
                     VEQGRVAVYAKRLAAEDPAKIVTALKAIPGVVSAEVHTGSAPEGAAGPGVVRATTPTA
                     ESKFLPRGLLVDPFHADLRWPHFGASYHSLSSGGREFASAFGESFSVYRDAAPFQGEW
                     ELGIQAGVFGVFDTEKRSIDLINADYTFGIVASYRADRFSGFIRIRHQSSHLGDEFLL
                     NNPQVVRINLSYEEIDLKMSYDLTTWLRVYGGVGTIVRKDPSTLGRETTQGGVELKSP
                     WLFFGGKVRPVAYADFQTHARTHWTTGQSIMAGLQFENARIGDRKLQVLGEYYSGPSP
                     DGQLFTQRVEWIGMGIHLWF"
     gene            complement(9293..9892)
                     /locus_tag="PK589_03745"
     CDS             complement(9293..9892)
                     /locus_tag="PK589_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ18737.1"
                     /translation="MRQGVLSAALVLCASFLIAGGVVVPSPARAADDKGLFEQMDIGL
                     LSIGGRATYIDPKDGSSRWFGGGQVRLHPWRYLAFEGSADYRRNDIGDTRVHTYPVQV
                     SALIYPLGTTRLAPFLLGGGGWYYTTVKGPGGFDDTQNRFGAHAGGGLQFFFNKHVSI
                     DSTYRYIWLEKIESRDQNIVDKKFQDNGHMVTVGVNFHF"
     gene            complement(9946..10287)
                     /locus_tag="PK589_03750"
     CDS             complement(9946..10287)
                     /locus_tag="PK589_03750"
                     /inference="COORDINATES: protein motif:HMM:NF017353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNJ18738.1"
                     /translation="MRLGHWRLSIVALAGILMMTAAGHAGEQHRLRGTQGTLILAVNQ
                     DQLAGMWKQFMGELKQKWAEFTDDDLMLIEGRIDTLEGKIQERYGDRREEVKRWVDEW
                     FESHGEKRTQS"
     gene            complement(10356..10634)
                     /locus_tag="PK589_03755"
     CDS             complement(10356..10634)
                     /locus_tag="PK589_03755"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNJ18739.1"
                     /translation="MKKMIAIALLSGVIASPAWALFETNKQLAATATVTLEDAVKHAL
                     KLVPGKAVEAEIGKQDGRTVYEVEIIDSNNKEQKVYVDAQSGQAKIDR"
     gene            complement(10688..10933)
                     /locus_tag="PK589_03760"
     CDS             complement(10688..10933)
                     /locus_tag="PK589_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNJ18740.1"
                     /translation="MNTDQFKGKWVQFKGEVKKQWGKLTDDDLKQVEGDYDKFLGRIQ
                     ERYGDKKEEVMRWADDWYSRQGQAQQGTQHRETQPPR"
     gene            complement(11588..11848)
                     /locus_tag="PK589_03765"
     CDS             complement(11588..11848)
                     /locus_tag="PK589_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18741.1"
                     /translation="MKVWPGRPYPLGATWDGEGVNFALFSENTTSVELCLFDGPDATR
                     ESHRIRLEERTNQTWHVYLPEGRPGLHYGHCVAACRWQRNCG"
     gene            complement(11906..12445)
                     /locus_tag="PK589_03770"
     CDS             complement(11906..12445)
                     /locus_tag="PK589_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNJ18742.1"
                     /translation="MKTEKPQASMIKDVQSIRERARQHIEEGALTADYKADRDTVIKL
                     LNEALATEIVCVLRYRRHYFMAQGMNAESVKAEFLAHAGEEQAHADELAERIVQLGGE
                     PNLSPDGLSSRSHSEYVEGEDLEEMIKEDLIAERIAIESYRDMIAFVGSDDPTTRRLL
                     ETILMKEEEHAEDLVSLLK"
     gene            complement(12485..12676)
                     /locus_tag="PK589_03775"
     CDS             complement(12485..12676)
                     /locus_tag="PK589_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18743.1"
                     /translation="MMPVSLSGNQFGKYMGLLISIAACALVVGTQARTVTQRTDEQGL
                     MTELGLESAWPHVRTDFSR"
     gene            complement(12795..13283)
                     /locus_tag="PK589_03780"
     CDS             complement(12795..13283)
                     /locus_tag="PK589_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ18744.1"
                     /translation="MTSTSSKSNVPRTIAEAGFTRPRRRLYLETRIDEDLGVLPGEVR
                     KVRRVMSSALTVVSPDTSHEEAVSLMAAMNVPVIVVYEGTRLVGMVTDRDVALHRSSR
                     PPTTALTVRDIMSEQVPYCQEDDLLTDAQALMRLSEVDWMPVLDRQGRLAGVLSIYVP
                     PS"
     gene            complement(13313..13609)
                     /locus_tag="PK589_03785"
     CDS             complement(13313..13609)
                     /locus_tag="PK589_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18745.1"
                     /translation="MESSEKAASIRQWINPEERVTVDFENEHDLNAEVIECDGQTVTL
                     LLETAFPHYKQHLTLPLSMISVGEDKSHYTRDPDKPVQYGRLRITVHEARPQAV"
     gene            complement(13909..14544)
                     /locus_tag="PK589_03790"
     CDS             complement(13909..14544)
                     /locus_tag="PK589_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNJ18746.1"
                     /translation="MAVTQPYQAKAFDLFGLSGISDETLRVHFALYEGYVKAANELRE
                     QLSEFIQQGHVDHEQMPAYSELTRRLGFEFNGMVLHEYYFGNLRRGGGAEPPNGSAFH
                     RAVERTFGSYERWKADFCSVGMMRGVGWAICNVDPARGLVSNHWVSLHEHGNVAGFLP
                     ILVMDVWEHAFLLDYQPSERKQYIDSFFSNIGWGVVEDRLQAGLSSVAIPH"
     gene            14839..15495
                     /locus_tag="PK589_03795"
     CDS             14839..15495
                     /locus_tag="PK589_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ18747.1"
                     /translation="MARARIVIADDHSLVLEAYRQLLEPDFDVVGTASNGQELLNIAP
                     ALNPDIILLDISMPTMNGLEVTKQLRAAVPHAKIIFVTMMSEPFYISQAFQMGASAYV
                     LKQSASTELLSALNAALKNQRYISPQLSLEVQDAIETPWVKPEGFSSKLTPRQQEVLQ
                     LLTKGMSTKEIATTMHVSTKAVEFHKGNITRRLGIRTTAELTRFALSQGLTKLSDQHP
                     "
     gene            complement(15505..16188)
                     /locus_tag="PK589_03800"
     CDS             complement(15505..16188)
                     /locus_tag="PK589_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ18748.1"
                     /translation="MKPRVLIADDHTLVAEGVEKLLEHDFQLCGRAADGRALVRAVEK
                     EKPDIALVDIALPLLNGLDACRQIKKSVPEVKLLVLTMHSEQYFVTEAFRAGVSGYVL
                     KQSVADELVFAIKEVLKGRLYVSPSVAENLVAQALHPVAPAPAGAPPSNDSLSGRQRE
                     VLQLIAEGQSTKEIASTLNVSIKTVEFHKTRIMKQLGIHSTAELTKHAIAIGLIAMPQ
                     QPTVPGSQP"
     gene            complement(16277..17569)
                     /locus_tag="PK589_03805"
     CDS             complement(16277..17569)
                     /locus_tag="PK589_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18749.1"
                     /translation="MSCSIVCLFSVGAIGDDLFGLGQDFALAEEPTPIAGEPFRTTLF
                     GEEIYVPPRDRRSVTAISFGIQAIPNGPTQQEVLPFGALYVWRNWDDDNRRLRGTFSG
                     VVNDVDYTIGLRDYPNWSLLFTWDNFILPFGRSEYVQGQRQRGTELEWSYAFAGAGLG
                     YRLPVHPFRQDSAVNISLTYEAGYRWFKGTGHTSADYGVPKDTYEGRIHFRIRADALK
                     RNLMELPHEGFTLGGDVVHGHRAKWDQWGGAPFNTQDFKKEQTYIQASAYALLATGLP
                     FIESDKHRLLSSIHAGIGKDLDRFSAFRLPGRPTGYEWEAISLPMIHGVAFNELFPTR
                     YAVANLQYRYEALFFLYPYVEATWGLVERPVFTSNTAVKSITDSMPALGGGLVTGAPW
                     KSQVELNYTYNFGIYRDPGGAPPTKGGHGIIFFWSKEL"
     gene            17850..18713
                     /locus_tag="PK589_03810"
     CDS             17850..18713
                     /locus_tag="PK589_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18750.1"
                     /translation="MERTYRTQPHCFNGTSAHVPRWRCCLAAAGLFLLTSTTLFANPI
                     EVIYPEGVSEGFVTLKTLDGKKLADGELSQVTTGADRLASRLTFRFADGSLYDETVTF
                     SQKKHLTMLSYQLTQRGPAFPEPLVISLNGETGQYQVRQREKTPDEKLITGRLDLPKD
                     VYNGLTITVLKNLRGQTGAIVHMLVFNPEPKLYEVELARVDEEDTRNTTRKETKATAV
                     HYSLKPRLGWFTAALARLLGRTPPDYHFWLIKKDAPAFVRFEGSLYAGGPSWRIEQVS
                     PRVKQARVPEP"
     gene            complement(18721..>19416)
                     /locus_tag="PK589_03815"
     CDS             complement(18721..>19416)
                     /locus_tag="PK589_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18751.1"
                     /translation="SNGRAIVMIGIMLGGIVCGEAGPFLVWSAEPVLSVAAAQHDQVH
                     GALAAEGTVDRFILDPRGEVEGLFLADGTHLYVTSRAADQLVRAFAPGNAVRVYGRLM
                     PDERLVQADAVTNVTTGTTFTVPLRLDLPMQEQEHTLSMTEMSAAGTIRAFFYHPLKH
                     TIQGMLLSDETQVRFPPDASPALRRSFHVGEHVRVRGNGTINRFGRAVEALAIGRNSA
                     ALVPLDASLQRLP"
     assembly_gap    19417..19516
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19608..21404)
                     /locus_tag="PK589_03820"
     CDS             complement(19608..21404)
                     /locus_tag="PK589_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNJ18752.1"
                     /translation="MNATAKIEDYALIGDTHTAALITRQGSLDWLCLPRFDSPACFAA
                     LLGTPEHGRWLITPHEDNTTIRRRYRGDTLVLETLFETPTGTAMLIDFMPPRDRDINV
                     VRVVVGVKGTVRMKMDLTLRFDYGSIVPWVSHQRRFLKAVAGPDAVYLETELELQGVG
                     FSTVADFTVTEGQEIPFTLTWRASHDSPPRPLITERALSQTELWWKKWSQRCTYQGSW
                     RNHVVRSLITLKALTYAPTGGIVAAPTTSLPELLGGVRNWDYRYCWIRDATFTLYALL
                     SAGYHSEAKAWREWLLRAVAGKPSDLQIMYGIRGERRLTELELNWLPGYEQSAPVRIG
                     NAASQQFQLDVYGEMMDTLHQARELGLAASEDAWRVQHAIMEFLETAWDRPDEGIWEV
                     RGPRRHFTHSKVMAWVAVDRAVKAIERCHVDGPLERWRALRETIHEQVCREGYDSNRG
                     TFVQYYGGTSLDGSLLMIPLVGFLPAFDPRVQGTLHAIERELTVDGLVMRYQPTGKLD
                     GLPGEEGMFLPCSFWLVDNLLLSGRVEEARRLYERLIGLSNDLGLLSEEYDLKNKRLV
                     GNFPQAFSHVSLIDSAYGFSRMESPAERRQRS"
     gene            21504..21830
                     /locus_tag="PK589_03825"
     CDS             21504..21830
                     /locus_tag="PK589_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18753.1"
                     /translation="MRKVLAKKTQPRKKTSSAKKRTVKRDIEPAAHVIERKTKKKPAT
                     SAKAKKTTLTKKKTSKTTRKRSPGSSRNAKSVGRAVGKVIGRAIGTVERALTLVRPNS
                     NTPKKQ"
     gene            21952..22275
                     /locus_tag="PK589_03830"
     CDS             21952..22275
                     /locus_tag="PK589_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18754.1"
                     /translation="MAFQVVSKLVSTTLFVALALPAALAAANPAPESGQPQPPSPQQT
                     PPTAPPSRIDPGIQKAPDTVPNPKSIVPPPVVDPKMAIDPERPGGPAHPKPPSPTDKP
                     PMPPR"
     gene            complement(22333..23604)
                     /locus_tag="PK589_03835"
     CDS             complement(22333..23604)
                     /locus_tag="PK589_03835"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNJ18755.1"
                     /translation="MNQQSRNPVVIVGGGFGGLAAARALHHLKGEVILIDRMNHHVFQ
                     PLLYQVATAALSPADIAWPLRTLLRAQQNVRIMMDEVRAVDRSARRIELRNSEPVSYS
                     SLILSPGARHSYFGHNTWEQDAPGLKTLPDALEIRERMLMAFEKAERLKGTPEARDEL
                     TFAIVGGGPTGVELAGALAEIGRKAMTPDFPVLHQAASRILLIEGGPRILEAFPPALS
                     SRAQASLESLGVTVLLNCRVHDVTSKGVMAGSEFISSAHVIWAAGNVASPILASLDVP
                     LDSSGRVIVGPDLSLKEDPWVFVIGDAAHCLSAQQKPLPGLAAVAMQEGRYVAEIIAG
                     CVPEGQRSAFRYRDRGILATIGRAKAVAQFGPISLSGVVAWLAWCFVHIFFLIGFRNR
                     ARVMFEWIWYYVTFKPGARLIYWKDRRINPQ"
     gene            23862..24950
                     /locus_tag="PK589_03840"
     CDS             23862..24950
                     /locus_tag="PK589_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ18756.1"
                     /translation="MKIAQVAPLWESVPPVLYGGTERIVSFITEELVRQGHEVTLFAS
                     GDSTTRARLMPMCGEALRPSKVVMNYEAPLTAMIEKVFGSAPEYDLIHSHLEFIPFPL
                     AKRCRTPMLTTLHARLDQPELIPVFRKFADLPLVSVSEAQREPLPWANWQATIHPGIP
                     HSLYTPQYNQGQYLVFLGRVAPEKGLDRAIEIAKRVEMPIRIGAKIDAQHAEYYKSVI
                     EPLLDHPLVEFVGEVTDLEKDDLLGDAYALLAPYAWPEPFGLAIIEALACGTPVIGSR
                     RGAIPEIIEHGVTGFVCDTLDEMTQAIRHVSLLERTRCRQAFETRFTAERMAQEYVTV
                     YGQILGLPDKAPQPSSDLASGLPAAGQI"
     gene            complement(25023..25736)
                     /locus_tag="PK589_03845"
     CDS             complement(25023..25736)
                     /locus_tag="PK589_03845"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNJ18757.1"
                     /translation="MNRRMAMYAGTIALALMTGTPAFSQVSTGSGAPPPDSTMGKGAG
                     TSTPKESPSFDKKTDLTGGKQGVPEEYADTPVRQGKLVEMKDSKYLNASVYGTNGEEI
                     GKIQQVMKDEKTGDIEYVVFVSNESKRPQPIRWSQFKTKGDKLQLTLKKEEIQNALPM
                     SSSKDKSPDLQEYKDKMGQVREAPTVPGSAGVPGQKGPDATGSMGEESVGGGGPSGTS
                     GLPSGKAPGFEGGNPSSKR"
     gene            complement(25790..26152)
                     /locus_tag="PK589_03850"
     CDS             complement(25790..26152)
                     /locus_tag="PK589_03850"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNJ18758.1"
                     /translation="MMATHIGRRLLTASTIEGTAVQNAAGEDLGEIHSLMIDLEKGRI
                     AYAVLSFGGFLGLGDKLFALPWGALTISPGGDFFILNVPRERLEQAEGFDKDRWPDMA
                     DTAWAERLHTYYGYKPYW"
     gene            complement(26342..29233)
                     /gene="treY"
                     /locus_tag="PK589_03855"
     CDS             complement(26342..29233)
                     /gene="treY"
                     /locus_tag="PK589_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HNJ18759.1"
                     /translation="MPRIPIATYRLQLNHTFTFRDAGRIVPYLYALGITDCYVSSILK
                     AVPGSMHGYDLVDPECLNPELGTEEDFAAFTAALKQYDMGLLLDVVPNHMGIRSTENR
                     WWWDVLEHGPGSRYATAFDIDWSPLKRELEDKVLLPILGEQYGAVLENQDIRLVYENG
                     GFIVTYFQHRFPVAPQSWEGILSFRLEELTARVGEQDEHVQELLSILTALRNLPARKE
                     RDPKRIAERYREAHMIRRRLAALLQESETIRVHLSATVDTYNGVKGDSASFDRLDALL
                     DEQSYRLASWRVASEEINYRRFFDINELAAIRTEETRVFQESHQLVFRLLKSGVATGL
                     RIDHVDGLYDPEHYLVQLQEWAARELEPAASGEPASLFVVVEKILGKGESLPVTWPVS
                     GTTGYDFLNLVNGLFVQSAKERSMDALYQRFVGQRIVYDDLVYLTKKLIMRASMSSEL
                     NVLGHQLNLLSEKDRQYRDFTLNSLTHAITELIACFPVYRSYITADQKDVLERDRTYI
                     MMAVNRAKRRNPTLNSQVFDFVRDLLLKRLDDRIKLAGPDQVRFVTKFQQTTSPVTAK
                     GIEDTAFYIYNRLVSLNEVGGEPAHYGLSVDAFHKALRERRAHWPHALLATSTHDTKR
                     GEDVRSRINVLSEIPERWRAALASWAKHNRRFRVKVDETVAPDRNEEYLLYQTLLGAW
                     PSTPMDQPQYEEFKKRIEQYMIKAIREAKVHTGWINPREDYEEGMKRFVQAILDRSVS
                     NPFLEEFLPFQAFVARCGMNNSLAQLLVKITAPGIPDCYQGTELWEFSLVDPDNRRPV
                     DFDQRAAILQDCQKHAGQDDVNRLALVRRLLESWEDGRIKLYLLQAGLRHRREHAALY
                     LDGDYVPLECDGVREANICAFARLHHEEAIVTVIPRLTAEVIQSDSAGAADTWQNTRL
                     TVPSWKPGSLYMNLLTGQRLETITEEGRQVLPINQVLRDCPVALLERCF"
     gene            complement(29258..30532)
                     /gene="glgC"
                     /locus_tag="PK589_03860"
     CDS             complement(29258..30532)
                     /gene="glgC"
                     /locus_tag="PK589_03860"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNJ18760.1"
                     /translation="MEKHGRISQPRVLAMILAGGKGTRLMPLTEARSKPAVPFGGAYR
                     IIDFVLSNFYNSGILGMHVMVQYRSQSLIEHLRKAWRIGDGERQFVTVVPPQMNSGAG
                     WYEGTADAVFQNLNLIRDFAPDIVAVFGADHIYRMDIRQMVQFHLDREADVSVATLPV
                     PVHAAQGFGIVEIDRTSLVVGFDEKPPVPKSMPGDPAHALSSMGNYLFNADVLIQALE
                     EDAKQEGGSHDFGRDILPRRLKTDRVMAYNFLDNEVPGVGYHEEPAYWRDVGTIQAYW
                     QANMDLLGDTPPCDLGNREWPIRTEPSYGPPASLVNCYVDHALIGEGTRAIEADIRRS
                     IIGRHVRIESGAYIEDSIIFDHTHIGAGAKLQRVVVDRHNTIPANVDLGPRGQERGEE
                     MDAPSEWAESGVLVIPKPQAATEEQKRRLPSY"
     gene            complement(30760..31323)
                     /locus_tag="PK589_03865"
     CDS             complement(30760..31323)
                     /locus_tag="PK589_03865"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNJ18761.1"
                     /translation="MIAIGREISPSTVQAYHQGEIRTLVLEELRGKWLVLMFYPGDFT
                     FVCPTELAELARLYPEFVTLGAEVCGVSTDSVYVHKAWHDSSPMVRTVEFPLIADSTG
                     ALSREFGVYLEEEGVALRGTFLIDPDGVLRAYEVHDNSIGRYIGETLRKLQAAIRVRE
                     GEGEACPANWQPGQPTLTPGLDLVGKL"
     gene            complement(31370..32824)
                     /locus_tag="PK589_03870"
     CDS             complement(31370..32824)
                     /locus_tag="PK589_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18762.1"
                     /translation="MRRKTTRTAGKAVSHLVSVGVVLAMSVAYVYADRDGKEPRHTVN
                     PAIEKTKEKGFEGVDFSYDLFGGPPGQSSARLAQEAMKKDQEEKPKVMAQQARLLQDR
                     YRLECKTHSTVRMTKGKPQPIGPTVQLKNGLTWESLSQMEPEEIRKQKAFPAGFMRLP
                     HVKHEVGGMVFPEEQIHQFPRLERFDVDLDLPACFLPEFPPPIFLTTHPELGDVSQGE
                     VLSVENFDRLFRGLITPVQLDGLRMLVTQFPQEEFNLTADRKSPKPSAGVACLDCHVN
                     FHTTGQFHLNPDTRPQRDRLRLDTVSLRGMFNQQIHGSKRSLRSVEDFTEFEQRTAYF
                     NGDHIRAAKKGISIIDRLQVAHMAQIQNMIDFPPAPKLDPMTGRLDRSRADRQEIAGE
                     DLFFGKARCAVCHQPPAYTDHMMHDLFLERFGAEADGPIKTFALRGIKDSPPYFHDGR
                     LLTLEDSVEFFNVVAGLQLNNDEKAALVAFLRAL"
     gene            33056..33277
                     /locus_tag="PK589_03875"
     CDS             33056..33277
                     /locus_tag="PK589_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18763.1"
                     /translation="MNCHRCSNLMFPVALQDWGGGLMNQDVAAWRCFACGEIVDQLIA
                     QNRTRGGEDAEEWRRGGARRRVNGIGLLR"
     gene            complement(33372..33572)
                     /locus_tag="PK589_03880"
     CDS             complement(33372..33572)
                     /locus_tag="PK589_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18764.1"
                     /translation="MTTLLVAVVLIGMILTGLYAFGTFSTPYTEAFRFGFYLLIALAL
                     VAVVLVVGRQPFEGYDPTPNSP"
     gene            34050..34892
                     /locus_tag="PK589_03885"
     CDS             34050..34892
                     /locus_tag="PK589_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18765.1"
                     /translation="MTVHMNRFLPHQGLHLATAVGISMMLSGVPVAMTSAHAAEETKT
                     GDTSQLREDAQTLLKGNRTVLATVLAVTSDQIKVDIGEVQPRFLPLKQAQQKGFPKVS
                     EGDDLIVVLNEQNLLVDYHPLDGGTSAHTIIRGELSQTLPTGHETAVIKSGGKEQSFK
                     IRSQARSKVAAIPRGATAVFLIDETNQIADVSLSRQQAAQLAGAHSQPIKGQHQQVEG
                     TVTSPLQSNHITIRTPGGTDKPYEVRDILQSKIATLHKGDAVVLLIDGDNKVIDVAIP
                     PKGK"
     gene            complement(34925..36292)
                     /locus_tag="PK589_03890"
     CDS             complement(34925..36292)
                     /locus_tag="PK589_03890"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ18766.1"
                     /translation="MTQARILIVDSNVGAATVLRDMLSVDGYEVSAVRDPKDAGGYLE
                     RIPVDLILTDLPPETEELTCLRQLRGLCPTTPIVLMTAHGSLRTAVEGLKAGAFDYMS
                     KPFTAEEVRSVARRAVEHTHMRAEQAASGEAAPDDTSPFASLVGSSPTMVQVYKAIAR
                     VAQTDSSVLLQGESGTGKELIARAIHASGPRSSGPFVTVDGGALADSLLESELFGHER
                     GSFTGAISSRKGLLEQAHSGTCFLDEVADLSPALQGKLLRVLQEKEIRRVGSTATTAL
                     DVRIIAASKLPLEPLVRAGTFREDLYYRMNVVTIDIPPLRERLDDLPLLAKRFLRLYG
                     SMKMPPVTELSAEALALLRRYWWPGNVRELEHAIERAVTLSPYSVICPEDLPPAVKDA
                     QPEGLARAQGWVTLEDLERNHILQVLDSHARDLGRSATILGIHRKTLLRKLRHYGLAA
                     GYGVA"
     gene            36841..37446
                     /locus_tag="PK589_03895"
     CDS             36841..37446
                     /locus_tag="PK589_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18767.1"
                     /translation="MKRWLACCASVIVTAIWFPCSSGALEFTADRVTHTDRGFHRARI
                     FYHDGMWRLEHNERGPVSITIVRLDRNQVWHLLPVTHHYKTVSYDRDYALHLATTLDH
                     EISRETIGVQILDGHPTVLYEVLTITPGGGRESYYQWIATDINFPLKLAKKNGDWMVE
                     YRHVKFGPISDVFFELPRHYAPMDDDQHTASSPEPVRRMSP"
     gene            37443..38000
                     /locus_tag="PK589_03900"
     CDS             37443..38000
                     /locus_tag="PK589_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18768.1"
                     /translation="MTTRTSRHQMTGARDHIVIGLLPNMGAVREALEALRARGISDDQ
                     IGLAMRQAEERLSDEESPSPTAQDATTGMVGGGLIGGLAGLLTTTGVVAIPGLAPLLA
                     GGSLIGLLGATGASIVAASGLGAVAGGLVGALISINVPETAAKHYADAVHEGQILISV
                     KTDGDPAQVEELLASLGATTDRQSP"
     gene            38127..38996
                     /locus_tag="PK589_03905"
     CDS             38127..38996
                     /locus_tag="PK589_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNJ18769.1"
                     /translation="MAKQQASRTKSNTPAAQKVVSQNGSAEPPVPHHEPIKTLVVDIG
                     GTGIKSMIVNEVGEPIGERLRLPTPKRKTPPSVLKTIHRLAKLSGRFHRVSVGFPGTI
                     RNGVILDAVNLDPSWKHFNLIAALGKTLRKKPVRAANDADVQGYGAISGKGVELVLTL
                     GTGVGSSLFIDGRLVPNLEVGKNRLRGSELERIGKKLWNNRLLKVIAKLEKMFHYDRL
                     YIGGGNAVQVDIGRVPGNVTVVSNLNGLLGGIALWEEAAHFAASRQELANNNANHSAP
                     PKPAAERAAPRGR"
     gene            39381..40061
                     /locus_tag="PK589_03910"
     CDS             39381..40061
                     /locus_tag="PK589_03910"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNJ18770.1"
                     /translation="MGDKERLISGLVGGGLLLHSLRGPFSGLSALKALLGLALVQRSV
                     TGHCAGYHMLGMNTHDQTDTSSIGRSKVHSDRAIKIERSITIQRSAKELYAFWRQLDN
                     LPTIMSQVRTVEPLDDRRSHWVINTLPGAPTVEWDAEIINEVENERIGWKTLDGATVE
                     HAGSVRFNPLNDERQTRVTVTLQYDPPAGAIGAAIADLFGQEPGQKIARDLERFKEAM
                     ESHTTVSQ"
     gene            40131..40451
                     /locus_tag="PK589_03915"
     CDS             40131..40451
                     /locus_tag="PK589_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18771.1"
                     /translation="MNMWRGLTVAVMVMGATVISGVPGGAAQKESMEKTPKVEDDTSD
                     KSAGKASKDKVIKKRRSSGKSNAEDTTDAKSQPKSQESREGTPGTTLMFRSDGAGNSG
                     AAGK"
     gene            40502..41629
                     /locus_tag="PK589_03920"
     CDS             40502..41629
                     /locus_tag="PK589_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18772.1"
                     /translation="MPKQNRHIRLFAAMLGLGIMSATGVYADQAREQREAGLQGQSDG
                     RVHNADITGGFYDDRYKLDDWYYDFYDSPGYSRADSTSVIQASNENTATMADAAHPSL
                     RHHRPYRTDQNVPATPYAGDPWFYAQRDPMYGIPETSSDAYGGNVIKGTIKAAKQVRN
                     RTTGDQNTVVLLTTPDRRQVTADLGSSRRTMDLALTKGDSLVVAGPWEDVGPYSVLMA
                     QQIRTEAKRVTLNRPIGAARGDRTVEGRIQQFRDIRMNRSGELHRAAAVQTPDGRMAL
                     VDLGPDNQDRTPLRAAAGDRMVAQGPVVQVGNYPVLWANRVAINDGMPVDVTRTTAPT
                     DFVPATGNRQENCLGAGCDNQPLNQGQPGNRNSNAMDGTTR"
     gene            complement(41847..42197)
                     /locus_tag="PK589_03925"
     CDS             complement(41847..42197)
                     /locus_tag="PK589_03925"
                     /inference="COORDINATES: protein motif:HMM:NF021568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsC family
                     protein"
                     /protein_id="HNJ18773.1"
                     /translation="MAQRSTNKADLEYALMMTILNFHTEFMRSNYANVQVHLSQDEIE
                     VTLTRQGSVPAERRLAESPEGRSLLRQVHEAMFLSCERILRERIEPVVERKVREMVTS
                     LDPVSGRSSITIRL"
     gene            complement(42418..42777)
                     /locus_tag="PK589_03930"
     CDS             complement(42418..42777)
                     /locus_tag="PK589_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HNJ18774.1"
                     /translation="MATTFSEAAKATRKKKVIKLVGVDMYIVTEEGIPKFPDGEFGPF
                     KCEFISNRGTKVWPGFVSPDLMMVNWYRCRFMATGDVTDADVDTFLAVLTKKGWWWSA
                     AQKLWTHDGEPAFSKAY"
     gene            complement(42813..43826)
                     /locus_tag="PK589_03935"
     CDS             complement(42813..43826)
                     /locus_tag="PK589_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNJ18775.1"
                     /translation="MAQHVVTMLPGEGTGPEICEAVRLVIDASGVDIKWEYEEIGLGC
                     LEKYGTLLPDKTIKNIAKNKVALKGPTTTPVGTGHKSANVTLRKVFDLFANVRPAKLI
                     PVLKRPWDKIDIINFRENTEDSYAAIEHMVSDEVAQCLKVITWPGSYRIADFAFQWAR
                     ANGRKKVYCVHKANIMKMTDGLFLDAFREAAKKYPEIETGDVIVDNCCMQLVRNPAQF
                     DCLVLPNLYGDILTDLCAGLVGGLGFAPGANIGDNCSIFEAVHGSAPKYAGMKKVNPS
                     AVLVSGIMMLRWLKETAAADRIEKAMFAVLHEGKHVTYDVGGTATTDEYAQAIVDKMQ
                     GKA"
     gene            complement(44078..45469)
                     /locus_tag="PK589_03940"
     CDS             complement(44078..45469)
                     /locus_tag="PK589_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNJ18776.1"
                     /translation="MRGRRTKTCPPSWTVPFSSYAEVTEFLRGVEVDCILRGQPSDGT
                     VSLGAVARCLHELKRPDLAYRSIHVTGTNGKTTVSRMIGALIRATGLTVGVYTSPHVT
                     NFRERIAINGEPIGEDELVDACNHVKAFLDVHGLQLAPFEFLTAAAFFAFRAAKVDYA
                     VIEVGIGGRQDATNVIAPELAVITTVEYDHADMLGDTLEQIAGQKAGIIKPFTPVLCG
                     PMPEGPRAVVLSRAAALKAPVLLSGQDYEVTEFIRDGYAGLCTVRVGASTHPQLRLRS
                     PAAFMAANAAHALMAYDVLRKRGLLPDIDPDEIGRVFEKTEWPACCEVLSGTPAVLLD
                     GAHNAPAAMRLATILRTTFEGRRSVLLVSLARDAQWEQVLRALAGAGADRLIVTGGPQ
                     DRAVPPDAIAEQWRTCSSVAAEVVDSTEAAFRRAVQAAGPHGVVAVTGSPQLAGYCRP
                     LAKTFPAQALVQP"
     gene            complement(45508..48789)
                     /locus_tag="PK589_03945"
     CDS             complement(45508..48789)
                     /locus_tag="PK589_03945"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HNJ18777.1"
                     /translation="MHTTNGSDQRHRDAQRMELRSYVSLAGETIAKVWPMTSVIARNP
                     LQGFEHLRFDEAVERAEQLFGGSGTLSGEAFREAFRRGRIQEKHFEEALRGQAGDRQI
                     TFGDRQVTHLDVLRAIMVAVGRDHDRVGTAATAPPTPTGDSVTVPHLAAWLGRMLPRE
                     TLETERAAGLQYIPGEWPCESTMADWCDRTLGVTITDEINRQMIKWCAAFCDEGEAAW
                     PMPKREETFFRAWKAAARYDFSLRCAGVERAASKIAALADRPEDVLLESLEMLHIPHA
                     IWQSYLSRHLAALPGWAGFIKWREDQDAHVWQSVYRIDLVKYLAVRLFYERELVATAC
                     RASLGCEGTASAVLEYGKRYPYTLWFRRASMSGDLPSALLPEASRLQRAGRSYDPAAW
                     EQDCRRWFEEQPAGERTYRVEAQNLVSLSGALAVPVHEITSTSPGDLAMLLRWMREFT
                     PAKQRVAWLEAFERSHQCEVLRKLELPAGEDARIAAPGAMTARPVAQFVFCIDVRSEV
                     FRRHLENRGGYETYGFAGFFGVPFSYRSLDEQRELPLCPVLLKPKHLIREVPRTYQDA
                     MAERRKHSSHLVKAGHELLHDLKHNVVTPYVMVEALGWFFLLPFLGKTLCPRWYHRFA
                     AWVKSWVVPPVATTLTVDKFSTADAENMLAAEQRLQIAWWLRKHGQRAGSLVTGAVLD
                     EVRRQALEEDEESASHPGALGRLLGVCSTEETDALVTLRRECRLTYRGTRSRLDRITR
                     TGFTVNEQAYYVETSLRLMGLTGSFARLVVLCGHGSTSQNNPYESSLDCGACGGGQGL
                     PNARAFAMMANRPQVREILASRGLTIPPDTQFVAALHDTTTDRVQVADLEDVPSTHRK
                     ELAQILDDVHEAGGEAAAERWVALTAAESHEGTRAARQELERRSVDWAQVRPEWGLSR
                     NSVFIVGSRRLTREADLNGRSFLHSYDHAMDGDGKLLEIIMTAPLIVAQWINMEYYFS
                     AVSPEVFGSGSKIYHNVTGGIGVMTGSQSDLRMGLPTQTVMRGEAPYHEPMRLTAIIE
                     APRERLSAIIERQPLLEKLFNNRWLLLIACEPAEAKYYRYDGGKWFVVADMAEQVGEA
                     PVPQA"
     gene            complement(49038..49835)
                     /gene="cysD"
                     /locus_tag="PK589_03950"
     CDS             complement(49038..49835)
                     /gene="cysD"
                     /locus_tag="PK589_03950"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNJ18778.1"
                     /translation="MRHLRELEDQSVYILREAYKHFDGLAMLWSMGKDSTVLLWLARK
                     AFFGHVPFPLVHIDTGYEMPELIEYRDRICRDWRLNLIVGQNKAALADGMGPDRGRVV
                     CCTAMKIDALNQTIAQHGWSAIILGIRADEEGTRAKERYFSLRGKEGEWNFRDQPPEL
                     WEQFQTKFPPGSHIRVHPLLDWTELNIWEYLEQERIELPSPYFDRGAGTRYRSLGCVP
                     CTGTVDSCASTIPQIITELRRTTTAERAGRAQDEGRGMETLRKLGHM"
     gene            complement(50173..51585)
                     /locus_tag="PK589_03955"
     CDS             complement(50173..51585)
                     /locus_tag="PK589_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ18779.1"
                     /translation="MAWHLLLLEDEASVREAFALRLRDKGYVVQTAETGEEALGLLRS
                     FEPDVLIVDLVMPNLSGLDVLARVKQMSPALPVIVLTARGTVKDAVEAMRLGAFDFVA
                     KSIDMDDLLHTLRRVTDFLTLQRQVQLHSGQDAARYALDRVIAKSPVTKSFLAQVQEL
                     VRNDRVTVLLQGETGTGKQYMGRVIHYNSARGSKPCVEVDCPSIPRDLFESELFGHEK
                     GAFTGAAGRKCGLIEMAEGGTVFFDEIGDLPLALQAKLLRVIEERSLRRVGGMNTVPV
                     DVRFMAATNRDLKAAVAKGEFREDLYFRLNVVMLTVPPLRARPEDIIPLAEHCLLRSA
                     LALKKPVRELTESARQLLQAYHFPGNVRELGNLIERAVIFCNGPSLDAAHFPADLAAA
                     VAPASLPGLPGAVQPTAAPAEADPLMVSLSFKVGERSLSDLEDAIIQAVLERSDGNKT
                     LAAKHLGITRWMLDRRRKAY"
     gene            complement(51666..53639)
                     /locus_tag="PK589_03960"
     CDS             complement(51666..53639)
                     /locus_tag="PK589_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ18780.1"
                     /translation="MASSRRSPTTRSRPVQSEIAAAEVLAWLSDCIQGGLSFVCKGIL
                     IFENSQFAELVESGLPRVEGHEHALRKRLLDDAIAWNNRPLGERKSVEYQLEGRDGGR
                     LYYVCRFNVVPYRGERGVLMVLEDVTERVMLAQDASHVARFQSVLARIGTLAVSGVSA
                     QELMNEAVKETAAALEVELCKILVPRAHDGQLYIMAGSGLQSELIGKLTIEGGTHSQA
                     GYAIAKRLPVVVDDLRRETRFSPSRLLTEHGAVSGMCVPMLVEDRVYGVMTAHSKRVR
                     KFSQQEQEFLCTMANTIGTVLERRWHEETQMDFYHRLFLSAQDGVMMTDTTGRILEWN
                     PALERMTGWTREEALGQRPAILKSGKHPPEFYDRLWASIRAGQPFVERFVNRRKDGSE
                     FLVWESVNPVKASDGTTQYFMAILTDLSEREQMLEALRHTEQVKLVGQLAGGILHEVR
                     NPLIGLGSLATHLADQASLPQEARDRCRLIAREAARIDELLESHLGQLRPRPFDITSC
                     DLASVLDDMLTLLRPNIVKNQILIKTSVAPDLPSVDASKAHILQVCLNIVLNAIDAMP
                     GGGHLHVTLVPEARRVPGVLMRFADTGKGIPADDLERIYEPFFTKGKAKGVGLGLTIT
                     RDMVERHHGQLVIQSPPGRGAVVDVWLPLKHEA"
     gene            complement(53752..54075)
                     /locus_tag="PK589_03965"
     CDS             complement(53752..54075)
                     /locus_tag="PK589_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNJ18781.1"
                     /translation="MLTLHPMKEIRIVVEGEHLKIVTSLLDRVGATGYTIINNVSGKG
                     HHGFHEGHLLFNDTSSQVIVFTVVPEERIEPILAGLGPLFHKHSGAMFVSDVAVTRRD
                     HFVTP"
     gene            complement(54093..57449)
                     /locus_tag="PK589_03970"
     CDS             complement(54093..57449)
                     /locus_tag="PK589_03970"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HNJ18782.1"
                     /translation="MAIETRAFTEAQRMELRSHVELAGEAIAQYWPMRTFIHHNPLHG
                     MEDMPFPQAIKRGEHLLGGKGYLSNAIYRRFFEQGRIGLNDVSCVLAPLASDRCVTFA
                     GRELTHLELMRVSMVHGLVDLPAKLQDSGAAGDTALDDLLAWLGTAVAGTRIRQPEPL
                     VPWESLDLLSHETLSAWCDQTLGTCIVSDINEQMIKWCSVFLDEGEASWVMPNREQTF
                     YRAWKRLARYDAGLGLLGIQDAATKIDALGERPEEAVLSCLVDLGIPKSAWEAYFALH
                     LSALPGWAGYIKWRAEEQHHPWQVRYPIDLVKYLAVRLFYEREFVEYHCRRALNRPGQ
                     YDAIRAILDQAPYAHWLRRQLTARTIPPWAIREAEEWRRSHQAATLDEWNQVGSRLYE
                     QTSEWRGTEAMKRDARRLLALAAALGTDPALISQTSVADAATVLNWLDGFPAGQQGQR
                     WLEALEAKHRRDVVDELRAVTKQLRATDDRAAPAGSARPLAQMVFCIDVRSEVFRRHL
                     EQLGGYETFGVAGFFGIPVKYQAFGEEHPVTHAPVLLKPKNHIREIPRSYHGAAASRH
                     RLFAQLSHVAHTLLHDLKENVITPYVMVEALGWFFSLPFFGKTLCPLAYQRLTSSLKS
                     LWVPPLATTLTIDKLTREEAEEMVAVEQRAVIRAALRQEFPALGSAITPALIDAIRVG
                     VMEDAEGQRGGDVAHILGISPVEVAALYERFRRDFNLTPRGMSSRLQRITQHGFSVSE
                     QAYGVEAALRLMGFTSGFSRLVVICSHGSTSDNNPYESALDCGACGGNSGLPNARAFA
                     SMANNAAVRKVLETRGIRIPGDTHFLAGLHDTTRNDVRIVDMEDIPATHRKDLVRLLE
                     DLREAGAQAAQERGLALGGPVATWSRKDPHTKAARRSVDWAQVRPEWGLSRNNLLIVG
                     RRELTRPLNLQGRAFLHSYDHRQDSSGKLLEAIMTAPLIVAQWINMEHYFSTVDNEVY
                     GSGSKVYHNIVGRVGVMSGANSDLRLGLPAQTVLDGSMPYHEPMRLLTIIEAPRTRVE
                     SVIATHPGLERLFHNEWVLLVVCEASEGAFYHYDIMRGWQPVSGLPEQSSAAAPRFVR
                     ESAPFLHQKDGETVGSVQEATAFEQKRMNP"
     gene            complement(57486..59150)
                     /locus_tag="PK589_03975"
     CDS             complement(57486..59150)
                     /locus_tag="PK589_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNJ18783.1"
                     /translation="MMEAIFPLVTILAPLFGTLSIALFWREMGERVSRLGIGALWASA
                     LTSLVTLALVSYGDPIRLTLAGAPSGAWSYTILVDRLAAVMMVLISSVSLVIHVYSER
                     YMVGDAGYTRFFALLGSLTFVLLSLVSSGNLLWMFACWHLVTWLLATLLVCNPASLAA
                     RDAGQKTFWVQSLGDAAFALVLLILYGSYGTLDVTELFPRVQAAPVTSWSGLALELNV
                     PLVTTLLLVLSVMTKSAQFPFHFWLIGTIEAPTPVSALMHAGIVNAGGFLVNRMAPLF
                     GLAPTTLHLLFVIGGLTALIGASAMLTQSSVKRTLVYSTMGQMGYMVMECGLGAFALA
                     VFHLCAHGLFKATLFLNSGAQIHKARTEFKLPPGNRVEESRAFSPMTWTTGLAVTLVL
                     PLIILLVAHGVVSIPLQESQGTVIFLFFGWVTTSQAMFSLYRLHAGASWNVSLTMLGA
                     LALIGLTYLWAGEAFTHFLYPAHGIAASYVQAAEWNRALFDAVVGVSAIMIVGAWGLI
                     YGKAKGIKVLMPGWMESLRTRAYVAFLNGLYIEDMVRMIGRAGSRR"
     gene            59522..60019
                     /locus_tag="PK589_03980"
     CDS             59522..60019
                     /locus_tag="PK589_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18784.1"
                     /translation="MLSAGQIRTSERRRIAHVLRGWMILWLLALPLFHIHPETDPHHG
                     EAGHVHAAAVHTVFSGDLEGEFGEHQETASVRPHPGEGPAVSAEGAHAWSSDPELNLS
                     LLNDATDRKLAKPLLTHLLFVAHHLVPLFDQQDGPHGEPLSSTVTMLFTRDVPARAPP
                     SPFFS"
     gene            60123..61373
                     /locus_tag="PK589_03985"
     CDS             60123..61373
                     /locus_tag="PK589_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ18785.1"
                     /translation="MMPRMVLSLLMACVFSQSGALSWAGEERPRAYSLSDVLTFAAQH
                     NPVLAGAEGLVKQSQGQQIAAGAYPNPSITGIAGRGAIRDPSTGTHVTERTLTVEQPL
                     EWTGKRQARQEAADAGLAGANAALEDARLSLLADVKIAFYHLLFGQRDVELSAQNVTN
                     VEEVLRTVQARVTAGEATSFDSLKAGVEVQKAKKEVARANNALLVARARLNTLTAGTL
                     GKEFSVQGDFDRPKQSLNADGLTAQALEQHPAVRRLSKLAEQADHTLRFEREARVPNV
                     SVIGSYHREAGDESLTAGLAVPLPLWYRRQGEIQSALGAKHRADAERLRAQQELEQAI
                     TQHAQEVRTAQEQLQVFETGLLKQAEQTLTVARTSFRHGAASLLDVLDAQRVYRQTQL
                     EYAQVRADLSIALARLERALGTSL"
     gene            61411..62574
                     /locus_tag="PK589_03990"
     CDS             61411..62574
                     /locus_tag="PK589_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ18786.1"
                     /translation="MTRRLMTIGMVPALLWLLSGCSDQGTPPPKPATTGEAQTITGSS
                     KAIHPPPAVQARLRSEPAALRAVPTLITAPGEVALDLKQVAKITSRIGGQVERIHVQL
                     GDRVKKGQALVAIGSLQLDQLIEEYLVGKAQADVAENSFRRTEKLRADDIVTERKLIE
                     DKGLYLETQARYQHIRERLSNMGISTDDLKQGPHEKSHLYTLTAPIAGTVVIQNAVRG
                     QGVGPGDELFEIVDTSRVWVFANLPIEQARQFKEGDSGTITPKGGEAIVAPLTYLSPV
                     ADETTRTIRVRFEVANLQGQLKPREYVEVALGWSGAPVITVPMTAITTIDKTRGLFLD
                     TADGYTFVPVDAGREGGGWVEIRRGVKEGDRIVTDGVFDLKNVLLKEHIGSGE"
     gene            62582..65704
                     /locus_tag="PK589_03995"
     CDS             62582..65704
                     /locus_tag="PK589_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNJ18787.1"
                     /translation="MQQLLTFSLRYRFFTLVAIVVVIASGLWSFAHLTIDAVPDLTPV
                     QVQILTRAPALGPVEVEQFVTFPIEASLNGLPALRELRSISRYGLSAVTAIFDDRTDI
                     YRARQFVAERLAQAMERIPAEYGRPVMGPLTTGLGEVYQFTVKGEGFSPMALRTLLQW
                     DIGMKLRAVPGVVEVNIWGGDPQQFHVIVDPSKLLSFKLSMKQVFDALQRNNAIAGGG
                     YIEHQREQLSIRGEALATQLSDLAQIVVAHGPGGVPIYIADLAEVKEGSALRIGAATA
                     MGEGETVIGMVQMLAGENAQEVVTRVKARVQDIQATLPAGVTIEPYYDRTIFVSKVMQ
                     TVRNNLLEGGLLVIAVLFLFLGDLRAGLIVASAIPLAMLIAFIGMMQAGLSGNLMSLG
                     AIDFGLLVDGSVVMIDNILRRLGEKPPPTREARLKEVLAAGQEVLRPMTLAVTIIILV
                     YVPILALTGIEGKMFRPMAVTVIMALTGSLFLALTVTPLLSFWFLRATPGSETTAVIR
                     GVRRVYAPWLQRAVARPIWPVSIAVGLFAVSLVAGSRLGIEFVPRLEEGDLAVQVWRL
                     PSISLSESVTTALDIERVLRRFPEVTRVVTRTGSPEVATDVMGVEMSDVFVILKPQRD
                     WVTAHSRNELIEAMKRQITDQVPGVGLGFTQPIEMRFNELIAGVRSDLAVKIFGPDLD
                     ILKQQAERAARALQHVPGAADVKVEQVAGLPLLRVVVDRAEIARYGLTAEEVLTLVQS
                     ARVGTIVGTVVQGSRRFELVVRLADRVSRDPGSLGSLLIPTMHGELVPLSRVTSILVD
                     SGPAQVSREHVQRRIVVETNIRGRDLGGFVSEAQQAVAQAVTLPPGYELIWGGQYEHL
                     QEAGKRLAMVVPVTLMLILGMLSVIFGTLRPALLIFLNVPLALSGGILALWLRGLPLS
                     ISAIIGFIALFGIAVLNAVVLVSHIRQLEAEGVSTREAVLHGAMDRLRPVLMTALVAS
                     LGFLPMALATSMGAEVQRPLATVVIGGLLTSTALTLLVIPALYSRLVPQAASRPHPAA
                     VQADDSAIQV"
     gene            65897..66262
                     /locus_tag="PK589_04000"
     CDS             65897..66262
                     /locus_tag="PK589_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ18788.1"
                     /translation="MATIMVIDDEPSIRGLLREVLERSGHKVVEAKDGREALDLYQKH
                     KADLLIMDLLMPEVDGLEATLQLTREYMDTKIIAMTGAQGDRNFLDIARLFGAHRTFE
                     KPFDLKEMLNAVEAELAQK"
     gene            66435..66833
                     /locus_tag="PK589_04005"
     CDS             66435..66833
                     /locus_tag="PK589_04005"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNJ18789.1"
                     /translation="MLIVLVGILPLHNFVGHAHWQYIRWVPTPDQLSSPAILLDLAFD
                     AVANILLFVPFGLLYALRGLEGKPLSPRHLLLMAFALSFSIEYYQVYCHNRSTSLLDL
                     LDNVLGAYLGMKAGEVYIRKNASAVVEPAA"
     gene            complement(66836..67243)
                     /locus_tag="PK589_04010"
     CDS             complement(66836..67243)
                     /locus_tag="PK589_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HNJ18790.1"
                     /translation="MACLCVLGLVLSGCAAPKPILYPNAHYQQVGADAAQADIQDCMA
                     LAKEAGASSSEGKTAQVVTGTVGGGAVGSAAGAVGGAILGHPGRGAMVGAASGATAGF
                     LRGLFKRSPPGAAFTNYVDRCLRDRGYDPTGWQ"
     gene            complement(67323..68180)
                     /locus_tag="PK589_04015"
     CDS             complement(67323..68180)
                     /locus_tag="PK589_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18791.1"
                     /translation="MFKIKKKIEKPKPPILYNVIEDYSEFDAPGNVTVCAMTLPEDLA
                     AHAKILAESYDVPLEQMLQLLTDGGVYVYPQVGGLLTRGLFACRVDPTGASPKQTWVL
                     DLMQFAEAEQWARARAVSLIDAAGEIFYRTLPEELKTKLAQKNLGLDYRTLDRAVAKG
                     GDIKFIDFRKDWSPHFKRLCIMPDGRLVETGGLEEFAQLHQITMPQAKKLVEEGGTMD
                     VAGGEVLACQIVNGQPAVARFSAKNYAKAKELVTAKGLHIMDALSEVAYNDSVMMRTL
                     QRKDLGGRI"
     gene            complement(68246..69991)
                     /locus_tag="PK589_04020"
     CDS             complement(68246..69991)
                     /locus_tag="PK589_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNJ18792.1"
                     /translation="MTLDQPSSGASAGTTSTDPIDRVIAYHIRTKHHFNRYARSLGFL
                     DWANQPNPFRRFEGAQLIRLPLLSPDDDPQSPSYDALYQPGAVASRPLTIKTISRFFE
                     LALGLSAWKRAGESEWALRSNPSSGNLHPTEGYVIVPPVDGLDLPPGLYHYAAKEHSV
                     ELRAGFPAAQITQLLEPFPDGAFLFGLSSVHWREAWKYGERAFRYCNHDVGHALGSAR
                     IAAATLGWRMVLLDGVEQNQVARLLGTHRQEDFGEAEPEHPDCLAVIWPSPNMKHDVQ
                     NLPLGVDQTVVKDLAPGPWHGHANLLSREHGVHWEAIDEAAEASWKTGEERLVVSLPV
                     EPPVHSEPRHDPQATRGAATIIRQRRSAVSFDGRTAISSATFFQILLRLIPHASQPQL
                     ARPMPWDLLPWDPAIHLLLFVHRVEGLTPGLYVMARDAKKLPLLQQAMNPELEWTPVP
                     GCPTDLPLYWLLQGNAQRLAAQVSCHQGIAGDSAFSLGMLAEFEGRLRQGGAWWYPRL
                     FWEAGLVGQVLYLEAEAAGVRGTGIGCYFDDPVHEIVGIKDLTLQSLYHFTIGGPVDD
                     GRLLTLPAYYHLQRG"
     gene            complement(70040..70735)
                     /locus_tag="PK589_04025"
     CDS             complement(70040..70735)
                     /locus_tag="PK589_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNJ18793.1"
                     /translation="MPATPHSEKHFTATATVRDIVIGMADGLTVPFALAAGLSGAVAS
                     SALVVTAGLAEIAAGSIAMGLGGYLAAKTDLEHYAAERKREVRETEHIPETEAEEVAE
                     IFRGYGLRDEHIAPLIASLRANPRRWVDFMMRFELGLEEPDPARARSSAWTIALSYVI
                     GGLIPLLPYMVFHEIATALWWSIAVTPVALFVFGYVKSGLTGVPPWRGGVQTVFVGGL
                     AAAAAFGIARLLG"
     gene            complement(70996..>71831)
                     /locus_tag="PK589_04030"
     CDS             complement(70996..>71831)
                     /locus_tag="PK589_04030"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNJ18794.1"
                     /translation="QSCSNVFTLPEIYFRVRDVVDNPDSTMDDLAQVLKMDPAISARV
                     LKIVNSPLYGFPKQVDTVTRAVNLLGMQAVRDLVTATTVGRSFSGMTVQIMDLSAYWR
                     KSVLCALMAGKIAKACGIEDSERFFIEGLLRDIGHLVLYQTIPERAQSALIEAGNLGE
                     PLAEVEQSNFGCDFTEVGAELVHSWGMPVQIEQAIRHQLCPEDAGDFSLHASMVHLAG
                     VVADHYELSPTRAPKDLPFQATALQTTRFNAEDRPMLLQEALDQLQDTVKLFSPAAMA
                     A"
BASE COUNT        15065 a        21974 c        20135 g        14557 t
ORIGIN      
        1 gtgacctccg ccgcgaaact acgtaactga tccaccatcg tattgatcgt atctttgagc
       61 tgcaggattt cgccgcgcac gtctacggtg atttttttag acagatcacc attcgcgacg
      121 gccaccgtca cgtcggcaat gttccgcacc tggttcgtga gattgctggc catggaattc
      181 acgctgtcgg tgagatcctt ccaggtaccg gccacgccgg gcaccaccgc ctgcccaccg
      241 agtttgccgt ccgtccccac ttcacgcgcc acacgggtca cttccgctgc gaaactgcgc
      301 aactggtcca ccatggtatt gatcgtgtct ttgagctgaa ggatttcgcc gcgcacgtcg
      361 gcggtgattt tcttggatag gtcgccgttc gcgacagcga tggtgacctc cgcgatgttc
      421 cgtacctggt tggtgaggtt gctggccatg gagttgacgt tgtccgtcaa gtccttccag
      481 gttccggcca caccgggaac cacagcttgt ccgcccagct tgccctccgt accgacctct
      541 cgcgcgaccc gagtgacttc ggccgcaaag gcatccagct gaccgaccat actgttcacc
      601 gttttggcgg tgctcaggaa ttcgccccgc agcggatgtc cctctgcttc cagggccacg
      661 gattgagtca ggtctccttt ggcgaccgcc ccgatgacac gcgccatttc tgtggtcggc
      721 cggaccaggt cgtcgatcag accgttgacc gcttcaatat tagttttcca ttctccccgg
      781 gcttcaggaa ccgacgcgcg ttccgacaac tttccttttt tgcccaccgc atggctgacc
      841 cgcttcagct cctcagagaa catggcgttc aattcaatca agccattgat ttcatccgca
      901 atctttccag ccatcccatc ccactccgct ggcaggcgca cagaaaagtc gccctttttg
      961 acggcttgga gaatgttgag gacggccttg tcatggccct cctcatagag gaccccggac
     1021 tgttgtcgct tgccggatcg cgaacgcagc ttggtattca ttgaatccct ccgtccatca
     1081 ctgcagagca gcgccgcggt cgagaagacg acccacgggt tagaatcact gtagtgcgaa
     1141 acactataaa gggcgggatt atccgcaagg actgggggat accctagttc cctaggcttg
     1201 ggattgtcgc agagctttgt aggaagaaat cggattggag aggagtcgtg aggcggagga
     1261 tcaggctgaa cagacggctc gaatactggg ctcacggtgc ccgccgccca aggtcaggca
     1321 tgcgggctct ccgggtgcgt tcgttgattt accacatgac aattgagcgc cagcgatgtc
     1381 ctgcgtccgc cgtcggaatg gaaattctgg caatcccgcc ggtgaccgtc acccgagcgt
     1441 gcccaatata ggcgtcagtg ggaatgggaa ggtatcgttc gaccgttccg ccggtgagga
     1501 taatgccttg ctgcgcgaat tccacggcga cctgcatgga tgtattctcc agcaccggtc
     1561 gaaagtctgc atagacatta aagttaccat cgctttcgga cacttcgatg acgggttgat
     1621 ggagagaaag gattgcggtc gccccgcagt atgaagcgga ctgaccgagt gaagtcccta
     1681 agacggcacg gcctacgttt ggttcctgga ttggacgatt caattcattt atcatggcgg
     1741 ctcctttatg aatggaactg atgattgccc tgtgcttggt accttagcgg gttttcccag
     1801 acgagttact aggaactccc cttgattcag agtcgatgtc cgctagcact ttgcctagtt
     1861 gtgacacagc tgtcggcggc tctagagtgg cctgaaaaag tggtgtcgcg cttcccctac
     1921 tctggaactc aggaatatgt atatgccgag tcctgacgaa acatcccctt cgatccctca
     1981 acccggtttt cccttggatt tcatgctagc ccatctggca gccatcgtgg aatgttccga
     2041 cgatgccatc atgtccaagg atctcaacgg gatgatcacg agctggaacc gaggcgctca
     2101 acacctgttc ggctacacgg cctgcgaaat gatcgggcag ccggtgctac gcctgatccc
     2161 cgaggatcgc caggccgagg agccacgtat tttggagcgg ctccgccggg gagaacgtat
     2221 cgatcactat gaaaccgtac ggaaacgcaa ggacgggacc ttaattgaaa tctctctctc
     2281 cgtctcgccc atcaaggacc gcgagggacg gatcatcggg gcatccaaaa tcgcgcgggc
     2341 gatatcggaa ctaaagcagg gcgaacggaa tttagcgcat ctggtcgagg cgcttcctgc
     2401 cgcggtctat accacggatg ctgacggccg cattacgcgg tacaaccaag ccgccgtcga
     2461 tctctggggg cagcggcctt ccctcgagac cgctcgctgg tccggatcct ggcgccaata
     2521 ctgggcggat ggacgcccca tgccaccgga agaatgtccc ttggcgcaat cccttgccct
     2581 caatcgcccc gtaagcggat tggagaccat cgtcgaacgg ccggacggca ctcgcctgcc
     2641 ggtggcctct caccccacgc cgctgcggga tgcatccggc gctcctatcg gtggagtgga
     2701 tatcctcgta gatttgaccg agcaaaagcg ggcggaacgg gacatgcaga attccgccgc
     2761 tgaactcgaa cgccgcatcg ttgaacgaac aggcgagctc cttctctcgc aggaacgcct
     2821 gcgcgcattg gcctctgacc tcacgctgac cgaacaacgg gaacgccggc atctggctac
     2881 cgaacttcac gactatctgg cacaacttgt cgtcgccagc cggatcagac tcgcacaagt
     2941 cattccgacc attcacgatg ccacggcatc ggccgccctc acgaagatcg atgggatgct
     3001 cgaccaggcg ttgacctata cgcgttccct ggtcgccgag ctcagccctc acatcctgta
     3061 tcaattcggc ttgtcacaat ccctggtgtg gctgggagaa cagatgaagc agcacggcct
     3121 tgaggtggcc gtgaaaacta tggctccgtc attcaccctg ccggacgatc aagccgtgtt
     3181 gttgtttcag tcggttcgcg aactgctctt taatgtcatc aaacatgcca aagtcgaccg
     3241 cgccgcgatt accataaacg tggatgagga ccgtcgagag ttgtggatct gcgtggagga
     3301 tgaaggcgcc ggcttcgatg tgacggacct gagcgtcgca aaaaatacac gctgtaaatt
     3361 cggcctgctg agcatcagag aacggatgga gctgctcgga ggcgagtgcg aagtgtcgtc
     3421 cgttcccgga agcggcacgg tcgccatcct tcggcttcca ctggcatcag gcgccctcaa
     3481 gcagccgttt ccgctggccg ccgacacggc acagaccatc acgccttcta tagacgcctc
     3541 ttcgcgcaac attaacattc tgctcgtcga tgaccatgcg atggtgcggc aggggcttcg
     3601 aagtatcttg gacgcacatg acaatttgac ggtggtcggt gaaggagccg acggtcagga
     3661 cgccgttgtt atggcgcgct cgcttcgtcc cgatgtcgtc atcatggatg tgaatttacc
     3721 gatcatcgac gggatcgagg ccactcgcat cctgacccag gaacatccct cgatcatggt
     3781 catcggcatt tcggtccgca atgatccaca agtgcatgac gccatgacgg aggccggggc
     3841 ggtcgcgttt ctaccgaagg aatccgctgc tgaccagctc tacgaagtca ttctgcgcgc
     3901 atttcccgtc caggcctgag tccgacgtga ggccgccgaa ctaggggatc ccccagtagc
     3961 gtcccgcagc cgacagtcct atgctgtaag cggcttgatt gaatggggat tgttcgccca
     4021 cgaaacaccc atgccactca ccgctgttcc accttgagtc acgcccggag aggggtccgt
     4081 tgtcgcttgt cgaaggcggt aagacccgga ggcttgtcat ttacacatga cttcggtgct
     4141 gctgacgctg caacgtctgg tcccctccgc cgccggactc gccattggat ttccggtctt
     4201 cgcctttctt tgttgggcat acaacccgac attcctctac aacctgtcgc cctcggcagg
     4261 aacggttaaa ccatccacga tggccgccat atgtttctgc ggcctctcct tgtggctcca
     4321 ggccgatgag gccgcgatga cgcgagcgag gcggcgcatg gcgcaaggct ttgccggcct
     4381 cgccggcctt ctggccgccg tcacaatgtt ggaatatcta ttgtccctcg acctgggcgt
     4441 cgatctgtgg ctcgcctctg ttccggattt ggcggtggac tcccatcccg gccgaatggc
     4501 tcctgccaca gccttcgctc ttttcatgct gggcttggct ctgatccagc ttgattcgaa
     4561 acacagcggc caggaccagg ccagtcatta tctggcactt gcgggaggca ccattggggc
     4621 catcgcactg gtcgggtatc tgtacggtgt ccagacgctg tacaaggtcg gtacatccaa
     4681 taccatttcc atctttagcg ccgcgctgct cctggccctc ggcctgggta ttctgagcgc
     4741 ccgccccacg cgcgggttta tggccacctt aaccagcgac gaactggggg cggtgatgct
     4801 gcggcgcgtc ctgcccttcg ccattgccct gccgattctg gccggatgga tccgtatcgc
     4861 ggctcaacgg tcgggccgct acgattttaa cttcggggtc gcgctggccg tgtcggccgt
     4921 catgttgatc atcctggcct ttctctggtt attggccggg aatctcaatc gcagcgacgg
     4981 gcaaaagaag catatcctgc aaatactgca gcaacgcgag gcccggcacc gtctcgcgct
     5041 gaaggccggt cgtatgggaa cgttccacca ggatcttgat cgtcacaccc tggtcttttc
     5101 tcccgaactg gaacacatct tcggtttgcc tgcgatgagc ttcggcagta cgttcgccgc
     5161 attccaggat ctggttcatc ccgaggaccg ggcacatgtg ctcaagacca ttaactacgc
     5221 gattcaacac cggagcgcct acgacctcga atgccgcatc ctccggcagg atcctcccga
     5281 ggaagcctgg atcgcagtca ccggacaggt gttgcctgac tccaccggcc atccgaggca
     5341 aatcaccggg gtcatgtttg atatcacgga acgcaaacac tccgtgaccg ctctccagca
     5401 gagtgaacag cagctgcggg tcatcactga tgcgctgccc ggccttattg cttctgtcga
     5461 ttgtgacgag cgataccggt tcgtcaatgc gggctatgag caccagttcg gactgtcgcg
     5521 tgaccagatc gtggggcttt cggtgaagga tctcctcaag gaggactatt ccaccgttca
     5581 gcccttcatc cggcgcgtgc tgagtggtga acgcatatcc tttgaaacta ccctcctgga
     5641 tgagcggggt gagcagacgc tgttatcgac gtacgtgccg gaccgaaact ccgaaggcat
     5701 ggtggcggga ttctatgcgc tgatcacaaa tatcacggag cgcaaacgca ccgaatccgc
     5761 gctgagagaa agcgaagaac gcttcaggca tctctttgag caggcgtcgg acgggattgt
     5821 gaccgcggac atgaacggcc agtatctcga tgtgaacaat cgcgcctgcc agatgttagg
     5881 ctatacccgc gaagagctct tcgacctgcg agtccccgac ctgctggacc cgcatgacta
     5941 cccgcgattg agagcgatca aggccgaact gatccaagga catcgtcatt ccggcgaatg
     6001 gaaattacgc cgcaaggacg ggggaaccat ctccgtcgag atcagcgcga gattgcaacc
     6061 ggaccgccgc tggcatgcca tcgtcagaga catcactgaa cgcaaacggg ccgatgcggg
     6121 attgcgcgaa agtgaggcgt attttcgcat gctggccgat gccactccgg tgctggtctg
     6181 gaaagccggg acggatcggc tctgttcctg gctcaaccgg tcctggttgg agtacaccgg
     6241 gcggctgttg gatgatgaat tgggaaccgg ccggaccgac agcatccatc ccgacgaccg
     6301 ggacgaattc aacagggtct acaaccagca cttctctcgc caagagccct ttgaactgga
     6361 atatcgatta cgccggcaag acgggaccta cggatggatc ctggaccgcg gcgttccgct
     6421 gcttagcgcc gccggggtgt ttagtggcta tctcggcgcg gccctcgata tcacggaccg
     6481 gaagttagcg gaagaacagc tgcaacaatg gaccgtcgaa ctggagaagc gcgtggacga
     6541 gcgcacgcaa gcactcgtgc attcgcaagc gcgtctccgt gcgctggtat ccgatctcag
     6601 cgccacggaa gagcaggagc gtcgccggct cgccacggag ttacatgact atctcgctca
     6661 gttgctggtc gtcggccgga tgaaactcag ccaagcgaga ccgcaggtcc ggaatcccaa
     6721 aacggaacaa ctgttgagtg aagcggacga catgctcacg caatcgctca actacacacg
     6781 atccctcgtc gctgaattga gcccgcagat cctgtatcaa ttcggtttgc ctgccgccct
     6841 caaatggctg gcgggacaga tgaaatcaca cgggctttca gtgtccatcc aatgcggcat
     6901 cgatcagctc acgctcgctg aagaacgcgc ggtcatttta tatcagagtg tccgggagct
     6961 tctgttcaac gtggcaaaac atgctggaac cggcgaggcc atgatcacgc tggaccagag
     7021 tccgaccaat caggtgctga ttactgtggc ggacaacggg cgcggattca atccagccat
     7081 tcttgtagac accgagggcc atcatcctgg acggttcgga ctctttaacg tgcaagaacg
     7141 catcgaagcg atcggcggaa cactgagtct caaatcttct gaaggctgcg gaacgacggt
     7201 cacactgatt gtgcccctga agccctttcc agaatcggct gtcgagtctg acctccccgt
     7261 cgacggccat gtcccggatg tgctcaggac cccgtctcaa cgattacgca ttctgctggt
     7321 cgatgaccat gccatggtgc gtcaaggatt gcggagtgta ttggaaagtt ataccgatct
     7381 ggaggtggtg ggggaagccg gcgatggagg gactgccatc agtatcgcct ccgccttaaa
     7441 accggatgtg gtcgtcatgg atatcaatat gccgaacatc gacggcatag aggcgactcg
     7501 ccatatcctc agcgagcatc ccgatgtcgt ggtgatcggc ctctcggtcc aaaacgaacg
     7561 gcatatcgaa gaagccatgt tgaaggccgg ggccgcggtc tttgtcacca aagaacgggc
     7621 cgccggccaa ctctacgaag ccattgtgag caccgtgcga accagacgat gagcagttcg
     7681 cacgggtcac acagctggcg ggcgtcggcc catcaccatg ccgatcacgg ccaaaatcag
     7741 aaagatcacg aacaggacat acgcgatatg cgtggcggtt ccagccactc cggtgacgcc
     7801 cagcacacct gcaatcagcc cgatcactaa aaaagtcaca gcccagctca acatgatcgc
     7861 acctcatcta taggtgaacc catactccct tatctatcgg gagccccgct cgaatggtac
     7921 gcgtgctgcg taccacaccc tttatgcccg cagacacaac ttccggtcgg cgctgcggca
     7981 tttcgacagg catcggaaca aaactcacgg cctttttcgg cctcacaagc acaggcagga
     8041 tgcgcgcatg atttttttcg tatggttgcc tccgccatta cctcgtcctt tccggcggcc
     8101 gactccgcct tccttctcga gcctcttccc tttgctgatg ttagaaccac aggtgaatac
     8161 ccatgccgat ccactccacc cgttgagtaa acaattgtcc gtccgggctc ggaccggagt
     8221 aatactcgcc caggacctgc aacttccgat cgccgatccg ggcattttca aactgcaagc
     8281 cggccataat ggactgaccg gtcgtccaat gcgttctcgc atgggtttga aaatcggcgt
     8341 acgccacagg cctcactttt cccccgaaga agagccacgg actcttgagc tcgaccccgc
     8401 cttgagtcgt ttcccgcccc aacgtgctcg ggtcttttct cacaatcgtt ccgacgccgc
     8461 cgtacacccg caaccaggtc gtcagatcgt atgacatctt gagatcgatc tcctcatagc
     8521 tgaggttgat ccggacgacc tggggattat tcagtaaaaa ttcgtctcct aaatgtgaac
     8581 tctgatgccg aatacgaatg aacccagaga atctatcggc ccgatagctg gccacaatgc
     8641 cgaaggtgta gtcggcattg atcaaatcga tcgagcgctt ttccgtatcg aatacgccga
     8701 acacaccggc ttgaattccc agttcccatt cgccctgaaa aggagcggca tcccggtaca
     8761 ccgagaagga ttcgccgaag gcactggcaa attcccggcc gcctgatgaa agagaatggt
     8821 aggaggcgcc gaaatgcggc catcgtaaat ccgcgtgaaa cgggtccacg agcaatccgc
     8881 gcggaagaaa tttcgattcc gcggtcggtg tcgtcgcacg gaccacgccc ggccccgccg
     8941 ctccttccgg cgcagacccc gtgtgtacct cagcgctaac aaccccggga attgccttga
     9001 gagccgtgac gatcttcgcc gggtcctcag ccgccaatcg cttggcatac accgccaccc
     9061 gcccctgttc gacctgcacc accgctccag agagatggaa ctcatgttcc agcaggccgg
     9121 ccgcgtatcc ggcgatatac tcgtcggacg cggccatcgc cattcccgcg aatcccgcca
     9181 tcacgcccag gagaaagagg gccatcccaa taggcgatgt gcggctccta gtcccctctt
     9241 tcggacacct gtgccacaac ctgttccacc tcacagctcc gtccgtctca gcctagaaat
     9301 ggaagttgac accgaccgtc accatgtggc cgttgtcctg gaactttttg tcgacgatgt
     9361 tttggtcccg ggactcgatt ttttccagcc agatgtaccg gtacgtactg tcgatggata
     9421 catgcttgtt aaagaaaaat tggagaccgc cgccggcgtg agcgccaaac cggttttgcg
     9481 tatcatcgaa gccgcccggc cccttcacgg tcgtgtaata ccacccgccg ccacctaaca
     9541 agaatggcgc cagtctggtg gtgcccagtg gataaatcag agcggagact tgaacggggt
     9601 aggtatggac gcgcgtatca ccgatgtcgt tgcggcgata gtctgccgag ccttcaaatg
     9661 ccaggtaccg ccagggatgc aatcgaacct gcccaccccc gaaccatcgg cttgagccgt
     9721 ccttcggatc aatgtatgtg gcgcgaccac cgatggagag cagcccgatg tccatctgct
     9781 caaacaaccc tttatcgtcc gccgcccttg ctggggacgg cacgaccact ccgcccgcga
     9841 tcaaaaacga tgcacacagg accaacgctg cgctcaagac cccttgtctc attgtcgatc
     9901 tcctctgtaa atggcaatgt ggcctaggtg gcctagttga cttcgctagg attgggtcct
     9961 cttctcccca tggctctcaa accattcatc aacccagcgt ttcacttctt cccgccgatc
    10021 gccataccgc tcctggatct tgccttccag cgtatcgatg cgcccctcga tgagcatcaa
    10081 gtcgtcatcg gtgaactcag cccacttctg tttcaattca cccatgaatt gtttccacat
    10141 tcctgccaat tgatcctgat tgacggccaa gatcagggtg ccctgtgtgc cacgtaacct
    10201 gtgttgttcg cccgcatgtc ccgccgccgt catcatcaga atccccgcga gggcgacgat
    10261 tgacagcctc cagtgcccga gcctcatcgt gtctctcctt cgaagccgat ggcaaggcgc
    10321 accggcgtgt cacctgccgg tacgcctggt gcccctcaac ggtctatttt cgcctgaccg
    10381 ctttgcgcgt cgacatagac tttctgttct ttgttgttcg agtcgatgat ttcgacttca
    10441 tagaccgtgc ggccatcttg ttttccgatc tcagcctcta cggctttacc cggaacaagc
    10501 ttcaaggcgt gtttcacggc atcttccagg gtgaccgtgg ccgtcgccgc taactgcttg
    10561 ttcgtttcaa acagagccca ggccgggctg gctatgacac cggacaacaa tgcgatggca
    10621 atcattttct tcatagggcc gactccttcc gcgtcaggtt gagctttgta cgtatcctca
    10681 tatgtgttca gcgcgggggc tgagtctccc gatgctgagt tccctgctgc gcttgccctt
    10741 ggcggctgta ccaatcatcc gcccagcgca tgacttcttc cttcttatcc ccataccgct
    10801 cctgaatgcg gccgaggaat ttgtcgtaat ctccttcgac ctgcttcagg tcatcatcgg
    10861 tcaattttcc ccactgtttt ttgacttccc ctttgaactg gacccatttc cctttgaatt
    10921 gatctgtatt catcgtgatc ctccatagtg atgtatcatt gccgcggacg cttgcgcccc
    10981 tgaggaagcg gcgtccccgc gactcctctg tcgggcggcc tctttcagta cccggtggga
    11041 cggagatcga aaagcaagtc cggtttttcc acctcggtac aacaccacgg tattctcctc
    11101 ggcgttgtcg ccgagggtga cgacgatctt cgtccgcact ccgtggtctt tctgatgtgg
    11161 tttcataggc acctcccgac ggttgtccat ctttgttata tctccgaata gggaaatggt
    11221 tgactggaca aagccctagt tttaaaaggc cctcgcccct gacaggtcta cgagtacgta
    11281 atagtgcgcg cacgtctaga acgggggcat agcctcgccg gagaggcagt agtcgaaggg
    11341 agagcagcga gtgacggata agacttacga atcgagcacg gagtttaagc ggctgaggat
    11401 ggcgaccgat cgagcctggg tcgtgtaggg ctcccctccc ttaaaagtcg gagtcggagt
    11461 cggagccggg tccggcttcc ctggctccgc cgcacccggc gtgagccgcc aagtggcgga
    11521 tcattcagat gagggtaaca gaaactcgac cggttcatga tgcgcattcg agagcaccag
    11581 aaatgtatca tccacaattg cgttgccatc ggcatgcagc cacacaatgt ccataatgga
    11641 ggccggggcg cccctccggc aggtagacat gccaggtttg attggtccgt tcttcgagac
    11701 gaatccgatg cgattccctc gtcgcatccg gtccatcaaa gaggcatagt tccaccgacg
    11761 tggtattttc agaaaacagg gcaaaattca ccccttcccc atcccacgtg gctcccagcg
    11821 gatagggtcg gcccggccag actttcatat catctccatc cgaaacggac tgtgaatcga
    11881 gacgtcacag tagcgctcga cgtcatcact tcagaagact gaccaggtcc tcggcatgtt
    11941 cttcttcttt catcagaatg gtttccaaca gccttctcgt cgtgggatca tcactgccga
    12001 caaatgcgat catgtcgcga taactctcga tcgcaatccg ttccgcaatg aggtcttcct
    12061 tgatcatttc ttccaggtct tcgccctcca catactcgga gtggctacgg gaagataacc
    12121 cgtccggcga gagattcggt tccccgccta attgaacgat ccgttccgcc agttcatccg
    12181 catgggcctg ttcctcaccc gcatgcgcca ggaactccgc cttgacactt tccgcgttca
    12241 tcccctgcgc cataaaataa tgccgcctgt accgcaaaac acagacgatt tccgtggcca
    12301 acgcttcatt caagagtttg ataaccgtgt cgcggtcggc cttgtaatcc gcggtcaacg
    12361 cgccttcttc gatatgttgc cgggcacgtt ccctgatgga ctgcacatcc ttaatcattg
    12421 acgcttgagg cttctcagtt ttcatgtcga ctcctttcaa cgtaaaggca cggtcacgtg
    12481 gtggtcagcg cgagaagtct gtgcgcacat ggggccaggc cgattccagt cccaattccg
    12541 tcatcaaacc ctgctcatcc gtccgttgcg tcaccgttcg ggcctgggtc ccgaccacga
    12601 gtgcacaggc agcgatcgaa atgagcaatc ccatatactt accgaactga ttgcctgaca
    12661 gagacaccgg catcatgacg tctcctcctc tactgacaac agctgtgctc acactagaag
    12721 agaggcccag agggacgtac taggcagaac ccttggcaac acaggggatt cccctcggcc
    12781 acggacgagc gaagtcagga aggaggaaca taaattgaca acacccccgc caggcggcct
    12841 tggcgatcca gaaccggcat ccaatcgacc tccgacaggc gcatgagcgc ttgcgcatcc
    12901 gtgagcagat cgtcctcctg gcaataggga acctgctcgc tcatgatgtc tctgaccgtc
    12961 aatgcggtgg tagggggccg ggaactccga tgcaatgcga catcccgatc ggtcaccatg
    13021 cccaccaaac gcgtgccttc atagaccacg atcaccggaa cgttcatcgc cgccatcaac
    13081 gacaccgcct cttcatgact cgtgtccggc gacacgacgg tcaaggctga agacatcacg
    13141 cgtctcacct ttcggacttc ccccggcaac actcccaaat cctcatcaat tctggtttcc
    13201 agatacagac gccgacgcgg acgtgtaaag ccggcctcgg caatggtacg aggaacgttc
    13261 gatttgctgg atgtgctggt cattggtgca agattttcaa caattaacaa tttcagacag
    13321 cttgaggacg agcttcgtgg acggtaattc gaagccgccc atactgtacg ggcttgtcgg
    13381 ggtcccgagt gtaatgactt ttatcctccc ccacggagat catgctgaga ggaagcgtaa
    13441 gatgctgttt ataatgtgga aatgccgtct cgagcagcaa cgtcaccgtt tgcccatcgc
    13501 actcaatcac ttcggcattc aggtcgtgct cgttttcgaa atcgacggtc acgcgctcct
    13561 ccggattgat ccactgccga atgcttgccg ccttctctga agattccatg cgccgcctcc
    13621 gtgtcgccgc atgccgacgg ccgtcacccg cgaccgtctt tcgccaacga catcaacact
    13681 tctcgcatgc attcgctgat gccaccactg gcaataactc taggtcctca gccagctgcg
    13741 gaatcggcgg attgaccggt tgcggtggaa tgggtggagg aaacggctcc ggtggaaatg
    13801 gattcggacc aggatccgga ttcggattcg gttcaggaaa cggcgcgggt cctgggttca
    13861 tctgcgggaa cagattcgag tggcgacaat tcatagaagg cctttcattc aatgcggaat
    13921 agcgacggat gacaatccgg cctgaagccg gtcttcaacc acgccccatc cgatattgct
    13981 aaaaaaagag tcgatatact gtttccgctc actgggctgg taatccaaga gaaacgcgtg
    14041 ctcccacaca tccatcacga ggatgggtag aaagcccgcc acattcccat gctcgtgcag
    14101 agacacccaa tggttcgaca cgagccccct tgccggatcg acattacaga tcgcccatcc
    14161 cacacctcgc atcatgccca cactgcaaaa gtcggccttc catcgctcat agctgccaaa
    14221 ggtacgttca acggccctgt gaaaggcgga gccgttcggc ggctccgctc cccctccacg
    14281 ccgaagattg ccaaaataat attcgtgcaa caccatcccg ttgaactcga atcccagcct
    14341 gcgcgtcagt tccgagtagg ccggcatttg ttcgtgatcg acgtgtcctt gttgaatgaa
    14401 ttcagacaat tgctcgcgca attcgtttgc ggcttttaca tagccctcat acagcgcgaa
    14461 atgaacgcgc aacgtctcat cggagatacc cgacaatccg aacaggtcaa acgctttggc
    14521 ttggtacggt tgggtgacgg ccatgttgag cccctttcat cgtgcttaag atggacatac
    14581 ttcaccatag gcccccacct tgcctttccc aactaggcga tgccctggca taccatggcc
    14641 tacgcagggg gctttcctag gcgtcatctt tcataaatta cggcagtatg aactgaatgg
    14701 acccccgaag aatttaccgg ggaagagacc gtgcaaagac tagcgcagac ctgctaaggt
    14761 gaagggaccc tcgaaacgac gagagtgtca ccgcggagga gctggtaaag tagtcccacc
    14821 gagagaggaa ggagcaccgt ggccagagca cgcatcgtca ttgccgatga ccatagtctg
    14881 gtgttggagg cttaccgaca attgctggag ccggatttcg atgtggtagg tacggcttca
    14941 aacggccagg aactgctgaa catcgcgccg gccctgaatc cggacattat cctgctcgac
    15001 atttcgatgc cgaccatgaa cgggttggag gtcacgaaac aactgcgtgc ggctgtacca
    15061 cacgccaaga tcatcttcgt gaccatgatg agtgagccgt tttatatttc gcaggccttt
    15121 cagatgggcg cgtcagccta tgtgttgaaa caatccgctt ccacggaatt gttgtccgcg
    15181 ctgaacgccg ccctcaagaa tcagcgttac atctcgccgc agctctccct tgaggtgcag
    15241 gatgccatcg aaacgccctg ggtgaaaccg gaaggatttt cgtccaagtt gacgcctcgc
    15301 cagcaggagg tgctgcagtt gctgacgaag ggcatgtcga caaaagagat cgccacgacc
    15361 atgcatgtgt ccacgaaggc cgtggaattc cacaaaggca acatcacgcg ccgcctcggc
    15421 atccgcacga ctgcagaact cacgcgcttc gccttatcgc aaggcctcac gaagctgagc
    15481 gatcaacatc cctgacagat gacgttacgg ttgagagccg ggaacggtag gctgttgcgg
    15541 catggcgatg agcccgatgg caatcgcatg tttggtcagc tcggcggtgc tatgaatgcc
    15601 gagctgtttc atgatgcggg ttttgtgaaa ctccaccgtc ttgatggaca cattgagcgt
    15661 cgaggcgatt tccttggtgg actggccttc cgcgatcaac tgcagcactt cccgctgccg
    15721 gccactgagc gagtcattcg acggaggcgc cccggcggga gccggcgcaa ccgggtggag
    15781 cgcctgggca accagatttt ccgccacgct cggtgacacg tacagacggc cctttaagac
    15841 ttccttaatc gcaaatacca attcgtctgc cactgactgc ttcaacacat aaccggagac
    15901 cccggcgcga aaggcttccg tcacgaagta ctgctcgctg tgcatcgtga ggacgaggag
    15961 cttcacttcg ggaactgatt tcttgatttg ccggcaggca tcgaggccgt tcaagagcgg
    16021 gagtgcgata tccacgagcg caatatccgg cttctctttt tcaacggccc ggacgagggc
    16081 ccgtccgtct gcggctcgac cgcacaattg aaaatcgtgt tccagcagtt tttctacacc
    16141 ttctgcaacg agtgtatgat cgtcggctat gaggactcgt ggtttcatgg atatgctccg
    16201 ggctagtgga caagccgagt aggaactgct gtggaatcct accactctct tcagatgaat
    16261 acgagtggtt cgtcggtcac aattccttcg accagaaaaa aatgatgcca tgcccgccct
    16321 tggtgggcgg cgcgccgcct gggtcccgat agatcccgaa gttatacgtg taattgagtt
    16381 ccacctgcga tttccacggg gcccccgtca ccaatccgcc gcccaaggcc ggcatggaat
    16441 ccgtaatact tttcactgcc gtgttcgacg taaacaccgg acgctccacc aatccccagg
    16501 tcgcctcgac gtaggggtac agaaagaata aggcctcata gcgatattgc aagttggcca
    16561 cggcatagcg ggtcggaaat aattcgttga aggcgacacc atggatcatc ggcagcgaaa
    16621 tggcttccca ctcatacccc gtcggccggc cagggaggcg gaacgctgag aatcgatcca
    16681 ggtccttccc gatccccgca tgaatactgc ttaacagacg gtgcttgtcg ctctcgatga
    16741 atggcagtcc cgtagccagt aaggcgtagg cgctggcttg aatgtacgtc tgttcctttt
    16801 tgaaatcctg agtattgaac ggggcgcctc cccattgatc ccacttcgcc cgatgaccat
    16861 gcaccacatc gccgcccaag gtgaatccct cgtgtggcag ctccatcagg ttgcgcttca
    16921 gggcatcggc gcgaatccgg aaatgaatcc ggccttcata cgtgtccttc ggcacgccgt
    16981 agtccgcgga ggtgtgaccc gtgcccttaa accagcgata tccggcttca tacgtgagcg
    17041 aaatgttgac cgcgctgtcc tgacgaaagg gatggaccgg cagtcgatac cccagcccgg
    17101 cgccggcaaa cgcataactc cactccaact cggtgcctcg ctgacgttgc ccttgcacat
    17161 actccgagcg gccgaacggc aggatgaaat tatcccaggt gaacaagagc gaccagttcg
    17221 gatagtcgcg caacccgatc gtatagtcca catcgttgac gacccccgaa aaggtcccgc
    17281 ggagccgccg gttgtcatcg tcccaattcc tccagacata taacgcgccg aagggcagta
    17341 cttcctgttg cgtgggcccg ttgggaatgg cctgaatacc gaaactgatg gcggtgacac
    17401 tgcgccggtc ccggggcggc acataaattt cttccccgaa cagggtggtt cgaaacggct
    17461 cacccgcaat cggggtgggt tcctcggcca gcgcgaagtc ctgcccgagc ccaaacagat
    17521 cgtcaccgat tgcccctaca gagaacagac atactatgct gcaactcaca cggaaacacc
    17581 attcagagag agttcgaccc atcgattcta tggctcctga catcgcacgt gggattcctt
    17641 gacagccgat gtcctcttat atgtcagccg tgatacaccc ggcccctagt gaaacccctg
    17701 gcagaggggg tggaatagcc gagttctttt tcgtcgcagg tcgcgcaata catgggaaat
    17761 ttttgtcgca gccccctttt ctcgccaacc tctcaaacaa ggaatattcc cagtgttctc
    17821 ctgtgtggac caaagatagg ttcacgacaa tggaacgtac ctatcgaact cagccacatt
    17881 gcttcaatgg gacgtctgcg catgttcctc gctggcgatg ctgtctcgcc gccgccggat
    17941 tgttcctcct gacgtcgacc acactgttcg cgaacccgat tgaagtcatc tacccggaag
    18001 gcgttagcga aggatttgtt acgctcaaga ccttggacgg caaaaagctc gccgatggag
    18061 aactgagcca ggtaacgacc ggtgccgatc gactggccag ccggctgacc tttcgttttg
    18121 ccgacggctc gctctatgac gaaaccgtca cgttttccca aaagaaacat ctcaccatgt
    18181 tgagttacca gttgactcag cgagggccgg ccttccccga gcccctcgtg atttcgctca
    18241 atggtgaaac gggacagtat caagtccggc aacgcgaaaa gacgccggat gaaaagctga
    18301 tcaccgggcg cctggacctt ccgaaggatg tctacaacgg cctcaccatt accgtcctga
    18361 aaaatcttcg cggacaaacc ggcgccatcg ttcacatgct ggtgtttaac cctgaaccca
    18421 agctctacga agtcgaattg gctcgagtcg atgaggagga cacgcgcaac accaccagga
    18481 aagagaccaa ggccacagcg gtgcattaca gcctcaaacc ccgcttggga tggtttacgg
    18541 cagcgctggc ccggcttctc ggacgaactc cgcccgacta ccacttctgg ctgatcaaga
    18601 aagacgcccc cgccttcgtg cggtttgaag gcagcctcta cgcaggcggt ccaagttgga
    18661 gaatcgaaca ggtttcgccg cgcgtcaaac aagcccgggt tcccgagccc tagtgacctg
    18721 tcaggggagg cgttgtaacg aggcatccaa cgggacaagg gccgctgagt tccggccaat
    18781 ggccagggcc tccaccgccc gcccgaatcg attgatcgtc ccgtttcccc tcaccctcac
    18841 gtgctccccg acatggaagg agcgccggag tgcgggactg gcatcgggtg gaaaccgaac
    18901 ctgcgtttca tccgacagca acatgccttg aatcgtgtgt ttcagcggat ggtagaagaa
    18961 ggcacgaatc gttcccgccg cactcatttc cgtcatcgac agcgtgtgct cctgctcctg
    19021 catgggcagg tccagccgga ggggaacggt gaacgtcgtc cccgtcgtca cattggtcac
    19081 ggcatccgcc tgaaccagcc gttcatcggg catgagccgt ccgtagaccc gtacggcgtt
    19141 gcctggtgcg aacgcccgga cgagttgatc cgccgcccgg gaggtgacgt acagatgagt
    19201 cccgtcggcg aggaacaatc cttccacctc tcctcgtgga tccagaataa aacggtccac
    19261 tgttccctct gccgcaaggg cgccatgaac ctggtcatgc tgcgcggctg ctacggaaag
    19321 caccggttcg gccgaccaga ccaggaatgg tccagcttct ccacacacga tgccacccaa
    19381 catgatgccg atcattacaa tggccctgcc gttcgannnn nnnnnnnnnn nnnnnnnnnn
    19441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19501 nnnnnnnnnn nnnnnncaca cacgatgcca cccaacatga tgccgatcat tacaatggcc
    19561 ctgccgttcg attgacgcat tctccctttt tccctcaacc cgagcgttca actccgctgc
    19621 cggcgctcgg cagggctttc catgcgagaa aatccataag ccgaatcaat gagcgacacg
    19681 tgggaaaagg cctgcggaaa atttcccacc agccgtttgt tcttcaaatc gtattcctct
    19741 gacagcaatc cgaggtcgtt actcaacccg atcaaccgtt cgtacaaccg gcgcgcttcc
    19801 tccacgcgtc cagagagcaa cagattgtcc accagccaga acgagcaggg caggaacatc
    19861 ccttcttcac ccggcaagcc atccaacttc ccggtgggct gataccgcat gaccagtcca
    19921 tccaccgtca gctcgcgttc gatggcgtgc aacgtcccct gcacgcgagg atcgaatgcc
    19981 gggagaaatc cgacgagcgg aatcatcaat aaactgccgt ccaaggatgt tcccccgtaa
    20041 tattgcacga acgtcccccg attcgaatcg tacccctctc ggcacacctg ttcgtgaatc
    20101 gtctcacgca aggctcgcca tcgctcgagc ggtccatcga catgacatcg ctcgatggcc
    20161 ttcaccgccc gatccactgc gacccaggcc atcaccttgg aatgggtgaa atgccggcgc
    20221 gggccgcgca cttcccaaat gccttcgtcg gggcgatccc aggcggtctc aagaaattcc
    20281 atgatggcat gttggacgcg ccaggcatct tcgctggccg ccagccccaa ctccctggcc
    20341 tggtgcaggg tgtccatcat ttcgccgtaa acatccaact gaaactgctg ggaggccgca
    20401 ttgccgatac gcacaggggc tgattgctcg tacccgggca accagttcaa ctccagttcc
    20461 gtgagtctcc gttctcccct gatgccatac atgatctgca ggtccgaagg tttccctgcc
    20521 accgcacgca aaagccactc gcgccaggcc ttggcttcgc tgtggtagcc agccgacagc
    20581 aaggcataca acgtgaaggt cgcatcccga atccagcagt agcggtaatc ccaattacgt
    20641 acaccaccga gcaattccgg gagcgaagtg gtcggagccg cgacaatgcc gcccgtcggg
    20701 gcataggtca gcgctttcaa tgtgatcagt gaacggacca catgattccg ccaggatccc
    20761 tgataggtgc agcgttggga ccatttcttc caccagagct cggtctgaga taacgcacgt
    20821 tccgtgatga gcggtcttgg gggagagtcg tgggaggccc gccacgtcag agtgaaggga
    20881 atctcctgcc cttcggtgac ggtgaagtcg gcgacggtgg aaaatcccac gccctgtaat
    20941 tccagttcgg tttcaagata caccgcgtcg ggaccggcca ccgccttcag gaagcggcgc
    21001 tgatggctca cccagggtac gattgatccg taatcgaatc ggagggtcag gtccatcttc
    21061 atgcgcacgg tacccttgac tcccacgaca acacgtacca cattgatgtc gcggtcacgg
    21121 ggtggcatga aatcgatcaa catcgctgtg cccgttggtg tttcgaagag cgtttccagc
    21181 accagggtgt caccccgata ccgtcgacga atagttgtgt tatcttcgtg gggcgtaatg
    21241 agccaacgtc catgctcagg cgtaccgagc agcgccgcga agcaggccgg tgaatcaaag
    21301 cgcggaagac agagccagtc gagagacccc tggcgggtaa tcagcgctgc tgtgtgggta
    21361 tctccgatca gcgcatagtc ttcgattttt gccgtcgcgt tcataggcct ctcgcgtagg
    21421 tgcggtgacg tgattgatga ttcgttttaa actgtacagg cgggcactcg cctaaacaac
    21481 ccctaacatc tcaggaggac aacatgagaa aggttctggc gaaaaaaacg cagccgcgca
    21541 aaaaaacctc ttctgcgaaa aagcgcaccg tcaagcggga catcgaacca gctgcgcatg
    21601 tgatcgaaag aaaaacgaag aagaagccgg ccacatccgc caaggccaaa aagaccacgc
    21661 tgacaaagaa gaagacctct aagacaacca ggaaacgatc tcccggttcc agccgcaacg
    21721 ccaaaagcgt cggtcgggct gtggggaaag tcatcggccg agccatcgga acagtcgaac
    21781 gggcgcttac cctcgtcagg cctaacagca atacaccgaa gaaacaatag atagacggga
    21841 aaaggaacag agtcgctagg gggacgccta gtacggatgt tgtggggaaa cctctacact
    21901 gaatccaagc gaacgaggga cgaggacctc gtgatgcgtg gaggtggacc catggcattt
    21961 caagtggttt cgaaactggt gagcacgacg ctgttcgtgg ctctggcact gcctgcagcg
    22021 ctggccgccg caaatcccgc tcccgagtcg ggccagcctc agccgccctc gccccagcaa
    22081 acgccgccga ccgccccgcc gtcgcgcatc gatccgggca tccaaaaggc tcccgacacg
    22141 gttcccaatc ccaaatccat cgtgccgcca cctgtggtcg atcccaagat ggcgatcgat
    22201 cctgaacgac cgggtgggcc ggcacatccc aagccgccgt cgccaacaga caagccgcct
    22261 atgccgccgc gatgacatca tacggtccat tctcctgaat gcgaggtcgg cacgcaggat
    22321 ggaccgttca tctcattgcg gattgatccg gcggtctttc caatagatca gtcgagcacc
    22381 cggcttaaat gtcacgtaat accatatcca ctcgaacatc actctcgctc gattgcgaaa
    22441 gccgatgaga aagaagatat ggacaaagca ccaggccagc caagccacaa ccccggagag
    22501 tgagatcggg ccgaattgtg cgaccgcctt ggcacgtccg atggtcgcca gtattccacg
    22561 atcccgatat cgaaacgctg agcgttggcc ctcgggaaca caaccggcaa tgatctccgc
    22621 cacgtagcgg ccttcctgca tggcgacggc cgccaatccc ggcaacggct tctgctgggc
    22681 cgaaagacag tgcgctgcat cgccgatcac aaacacccag ggatcctctt tgagactgag
    22741 atcggggccc actatcacgc gccctgagga atctaatgga acgtctagcg aagcgagaat
    22801 gggtgatgcc acattgcccg cagcccagat cacatgggcg ctgctgatga attctgatcc
    22861 ggccatcacg cccttcgacg tcacatcgtg aacgcggcag ttcagcagca cagttacgcc
    22921 caggctttca agtgaggctt gcgccctgct cgataaggcg ggaggaaacg cttccagaat
    22981 acgcggtccc ccttcgatga gcagaattcg agaagccgct tggtgcagca ccggaaaatc
    23041 cggcgtcatc gcctttcgcc cgatctccgc cagcgcccct gccaactcca cccccgtcgg
    23101 gccgcccccc acgatcgcga aggtcagttc atcacgcgcc tcaggggtgc ccttcaggcg
    23161 ctccgccttc tcaaatgcca tgagcatccg ttcgcgaatc tctaatgcat ccggcagggt
    23221 ctttaaacca ggggcatcct gctcccatgt attgtggcca aaatacgagt gcctggcgcc
    23281 gggggagaga atcagactgc tgtaagagac cggctcacta ttccggagct cgatgcgacg
    23341 ggcggaacga tcaacggccc gcacttcatc catcatgatc cggacgttct gttgggcccg
    23401 aaggagcgtt cgaaggggcc aggcgatgtc agccggtgac agggctgccg tcgcaacttg
    23461 gtagagcaac ggttggaaca cgtgatgatt cattcggtcg atgaggatga cctccccctt
    23521 caagtggtgc agcgcgcgcg ccgctgccaa tcctccgaat cccccaccga ctatgacgac
    23581 cggatttcgg ctctgctggt tcactcgcgc ttctccatca cgcttacggt gaaaggcatc
    23641 acagcaggcg tctcgctgga cgcatcatct ctgcacgagc acaacggagc cactagggag
    23701 ccgcctagtt ctggaaaggc acgcgctctc gctatgatcc atctcgcagg agtggctctt
    23761 catcaccaca cattcttgtc ggaaacaaac gccagctacc tgcctttcac atcacaccca
    23821 acattcacac acatttattg tgccgggaaa aggagccagg tatgaagatc gctcaggtcg
    23881 ctcccttatg ggaaagtgtt ccgccggtgc tctacggcgg gacggaacgc atcgtctcat
    23941 tcatcacgga ggagctggtc cgacaaggcc acgaggtcac gctctttgcc agcggagatt
    24001 ccacgacaag agcccgactg atgccgatgt gtggcgaagc gctccgcccc tcaaaagtcg
    24061 tcatgaatta tgaagcccct ttaacagcca tgatcgaaaa ggtattcggg tcagcacccg
    24121 aatatgacct cattcattca catcttgaat tcattccttt cccgctggcc aaacgatgcc
    24181 gaacgccgat gctcacgacc ttgcatgccc gtctcgatca gccggagctc atcccggtgt
    24241 tccggaaatt tgccgacctt ccgctggtct ccgtctcgga ggcgcaacgt gaaccgcttc
    24301 cctgggcgaa ctggcaggcg acgattcatc ccggtatacc gcattccctc tatacgcctc
    24361 agtacaacca gggacagtat ctcgtgttcc tcgggcgcgt cgcgcctgag aagggactgg
    24421 accgggctat tgaaatcgcg aagcgcgttg agatgccgat acgcatcggt gccaaaatcg
    24481 acgcgcagca tgcggaatac tataagtcgg tcatcgagcc gctcttggac catccactgg
    24541 ttgagtttgt gggagaagtg acggaccttg aaaaggatga tttactggga gatgcctatg
    24601 cgctgctcgc tccctatgcg tggcctgaac cgttcggcct ggccatcatc gaagcgttgg
    24661 cctgcggcac accggtcatc ggatcgcggc gcggcgccat ccctgaaatc attgaacatg
    24721 gcgtgaccgg ctttgtatgc gacacactcg acgagatgac ccaagccatc aggcatgtgt
    24781 cgctgctcga acgcacccga tgccggcaag catttgagac gcgatttacc gcggaacgaa
    24841 tggcgcagga atatgtgaca gtctacgggc agatcctggg acttcccgac aaggctcctc
    24901 aaccttcatc cgacctggct tccggattgc ctgctgccgg ccagatctaa ttccaccttc
    24961 acgcacgaag ggcaggtact ctgttgagca cctgcccttc tcgaaccacc tacttcttct
    25021 acttatcgct tactgctggg attgccacct tcgaatcccg gagctttccc tgaaggcagc
    25081 ccgctggtgc cgctcggtcc gccgccaccc acagactctt cacccatgga gccggttgcg
    25141 tcaggcccct tttgtccagg gacgccggcg ctgccaggaa cggtcggggc ttcccgcacc
    25201 tgtcccatct tatctttgta ttcttgcagg tcgggagact tgtctttgct gctgctcata
    25261 ggcagcgcat tttgaatctc ctcttttttc aaggtcaatt ggagcttgtc gcctttggtt
    25321 ttaaattgac tccacctgat gggttgtggc ctcttggatt cattggagac gaagacgaca
    25381 tattcaatgt ctccggtctt ttcatctttc atgacctgct ggatcttgcc gatctcttct
    25441 ccgttggtgc catagacaga ggcattgaga tatttgctgt ctttcatctc aacgagtttc
    25501 ccctgtcgga cgggcgtatc agcatattct tccgggacac cctgtttccc gcccgtcaga
    25561 tctgttttct tgtcgaatga aggggattcc ttgggcgtgc tggtgcccgc gcccttaccc
    25621 atggtggaat ctggcggtgg cgcgcctgat ccggttgaga cctgactgaa ggccggcgtg
    25681 ccggtcatca acgccaacgc aatcgttcct gcatacatgg ccattcgtcg attcatgtgg
    25741 attctccttt gtatgagatg tcgtggtgga cgcccctact gatttggttt taccaatagg
    25801 gcttgtagcc gtaataggta tggagccgct cggcccaggc ggtgtcggcc atgtccggcc
    25861 agcgatcctt atcgaatcct tccgcctgct ccaaacgttc ccgcggcaca ttgagaatga
    25921 agaaatctcc tcccggggag atcgtgagcg ccccccacgg cagagcgaac agcttgtccc
    25981 ccaatcctaa aaacccgccg aaggacagta cggcataggc aatccgtcct ttttcgaggt
    26041 caatcatgag cgaatgaatc tcgcccagat cttcgcctgc cgcgttttga accgcggtgc
    26101 cttcgatcgt tgacgctgtc aataatcgac gcccgatatg tgtggccatc attttcctcc
    26161 tctaatgtta ggaatcactg catatgagtg ttcatgctaa tggcgctgtg cccttgtccg
    26221 tcgtccgtcg aactcatccc tctttcttgt agtgttaacc cccgagcctc actcgggcag
    26281 ctaggaagga ccctagaaaa taggaaggag aaatgacctg caggaaagcc tatcgctgcg
    26341 ttcagaagca ccgttccagc agggccacag ggcaatcacg tagaacctgg ttgatcggaa
    26401 gaacttgtcg gccctcttcc gtgatcgttt cgagacgttg gcccgtgagg agattcatat
    26461 agagtgagcc gggcttccac gagggaaccg tcaagcgagt gttctgccac gtatcggcgg
    26521 ccccagccga atcggattgg atcacctccg ccgtgaggcg agggatgacg gtgacaatgg
    26581 cctcttcgtg atgcaagcgg gcgaaggcgc agatattcgc ctcacggacg ccgtcacatt
    26641 cgagcggcac atagtctccg tcgagataga gtgccgcatg ttcacgccga tgacgaagac
    26701 ctgcttgcag cagatagagc ttgatacgac catcttccca ggactccagg aggcggcgga
    26761 ccagtgccaa ccggttgacg tcatcctggc cggcatgttt ctgacagtct tgcaggatcg
    26821 cggcacgctg atcgaagtcg actggcctgc gattatcagg atccacgaga ctgaattccc
    26881 acaactctgt gccctggtaa caatccggaa tccctggtgc agtgattttg accaatagct
    26941 gcgccaatga attgttcatg ccgcaacggg ccacgaacgc ctgaaacggg agaaactcct
    27001 cgaggaacgg gttggaaaca gaccggtcca ggatagcctg cacaaatctc ttcatgccct
    27061 cctcgtaatc ttcacgagga ttaatccagc cggtgtgcac tttcgcttca cggatggctt
    27121 tgatcatgta ctgttcaatc cgctttttga actcctcgta ctgaggttga tccatcggag
    27181 tcgagggcca ggccccgagc agcgtttgat acagcagata ctcctcgttc cgatcgggcg
    27241 cgacagtctc gtccactttg actcgaaagc gtctattatg tttcgcccag ctggccaggg
    27301 cagctctcca gcgttccgga atctccgaca ggacattgat ccttgagcga acgtcctcgc
    27361 ctcgtttggt gtcatgtgtc gacgtagcca acagggcatg aggccaatgc gcacggcgtt
    27421 cccgcagcgc cttgtgaaag gcgtcgacag aaagtccata gtgagccggt tcaccaccga
    27481 cttcattgag cgacacgagg cggttgtaaa tatagaacgc ggtgtcctcg atgccttttg
    27541 ccgtcacagg gctcgtggtc tgttggaact tcgtcacaaa tcgcacctga tccggtcctg
    27601 cgagtttgat ccgatcatcc aggcgcttca ggagaaggtc tcgcacaaag tcgaagacct
    27661 ggctgttcaa ggtgggattc cggcgtttcg ccctgttcac ggccatcatg atgtaggtgc
    27721 ggtcccgctc cagcacatct ttttgatccg ccgtgatgta ggagcgatag acaggaaaac
    27781 aggcgatcag ttccgtgatg gcgtgggtca aactattcaa agtgaaatcc cgatattgcc
    27841 ggtctttttc cgaaagcaga ttcaattggt gtcccagcac gttcagctcg ctggacatcg
    27901 aggcccgcat gatcaacttt ttggtgagat acaccaggtc gtcgtacaca atccgctgac
    27961 cgacgaaacg ctgatacaag gcatccatgg accgttcctt cgccgactgg acgaagaggc
    28021 cattgacgag gttcagaaag tcatagccgg tcgttccgga gaccggccag gtcaccggaa
    28081 gcgattcgcc tttgccgagg atcttttcga ccacgacgaa caacgaggcc ggctcccccg
    28141 aggcggccgg ctcgagttct cgggccgccc actcctgcaa ttggaccaag taatgttccg
    28201 ggtcatagag cccgtcgaca tggtcgattc gcaggcccgt ggccacaccg ctctttaaca
    28261 gcctgaacac caactggtga gattcctgga agacgcgtgt ttcttccgtg cggatcgcgg
    28321 ccaattcgtt gatatcaaaa aatcgccggt aattgatttc ctcggaagcc acgcgccagg
    28381 acgcgagacg ataactctgc tcgtcgagta acgcatccag ccggtcgaaa ctggccgaat
    28441 ctcctttcac accgttgtac gtatccacgg tggccgacag atgcacgcga atggtctcac
    28501 tctcctggag taacgctgcc aggcggcgcc tgatcatgtg cgcttcacga taccgttcag
    28561 cgatgcgttt cgggtcacgc tctttcctcg ccggaagatt gcgtaaggcg gtcagaatac
    28621 tgagcagttc ctgaacgtgc tcatcctgct cccccacccg cgccgtcaac tcttccaggc
    28681 ggaaggatag gatcccttcc caggattggg gcgcgacagg aaaccggtgc tggaaatacg
    28741 tgacgatgaa gccgccattc tcgtagacca gtcgtatatc ctggttctcc aataccgcgc
    28801 cgtactgttc gccgagaatg ggcagcagca ccttatcttc aagctcgcgc ttcaacggcg
    28861 accaatcgat gtcgaacgct gtggcatagc gagatcccgg gccatgctcg agaacgtccc
    28921 accaccaccg gttctccgtg gagcggatcc ccatgtgatt gggcaccacg tccaataaga
    28981 ggcccatatc gtattgtttg agcgccgctg tgaacgcagc gaagtcctct tcggttccca
    29041 gttcaggatt gaggcattca ggatccacga ggtcgtagcc atgcatactg cccggcacag
    29101 ccttcaagat agacgacaca tagcaatcgg taatgccgag cgcgtagaga tagggaacaa
    29161 tgcggccggc gtcacgaaag gtaaacgtat ggttgagctg aagacggtag gtcgcgatgg
    29221 gaatgcgcgg catacgtatc agagcgcgtg ccgccgttca ataggaaggc agtcgtcgct
    29281 tctgctcctc tgttgccgcc tgaggtttgg ggatgaccag cactcccgat tccgcccatt
    29341 ctgagggcgc gtccatctcc tccccgcgct cttgcccgcg gggtccgaga tcgacattgg
    29401 ccggaatggt attgtgccga tcgacgacca cgcgctgcag cttcgcgccg gcgccaatgt
    29461 gcgtgtgatc gaaaataata gagtcttcga tataggcgcc ggattcgatt cgcacgtggc
    29521 ggccgatgat cgaccgtctg atgtccgcct cgattgcccg tgtgccttcg ccgatcaaag
    29581 cgtgatcgac ataacaattc acgagacttg ccggcggacc gtaggacggt tccgttctaa
    29641 tcggccattc ccgattcccc agatcacagg gcggtgtatc gccgagcagg tccatattgg
    29701 cttgccaata ggcctgaatg gtgcccacgt cccgccaata ggccggttcc tcgtgatacc
    29761 cgacgcccgg cacttcatta tcgaggaagt tgtacgccat gacccggtcc gttttgagac
    29821 ggcgcggaag aatgtcgcgg ccgaagtcat gcgatccgcc ctcctgcttg gcatcttcct
    29881 ccagggcctg gatcaataca tcggcattga agaggtaatt ccccatggac gaaagggcat
    29941 gcgccgggtc gcccggcatg gatttgggca ccgggggctt ttcgtcgaac cccaccacca
    30001 gactcgtgcg atcaatctcc acgatcccga atccctgtgc ggcatgcact ggcaccggga
    30061 gtgtcgcgac cgatacatcc gcttcccggt cgagatgaaa ctgcaccatc tgccgaatat
    30121 ccattcggta aatatggtct gcgccgaata cggcgacgat gtcaggagca aaatcacgga
    30181 tcaggttgag gttttgaaag accgcatctg cggtgccttc ataccatcct gctccggagt
    30241 tcatttgagg agggacgacg gtgacaaatt gcctctctcc atctccaatc cgccatgcct
    30301 tccggaggtg ctcgatcaga gattgcgatc ggtactgcac catcacgtgc atgcccagaa
    30361 tgccggagtt gtaaaaattg ctcagaacga agtcgataat ccgataggca ccgccgaacg
    30421 gaacggccgg tttgcttcgc gcttcggtta acggcatgag gcgcgttcct tttccccctg
    30481 ccaggatcat ggcaagaacg cgaggctgac tgatccgtcc atgtttctcc ataacgctcc
    30541 tcggtaggca ggatgattgc agcgggcacg gccactatca ccttacaaaa aatgtcgccg
    30601 atcgcgtact ggacaaatcc ctagcgcact ctgcgcaaag ccaaccgcaa tcagcaatgt
    30661 caggagtgaa atgattcctc gctgcaggaa gaagagaacg gatcgggata cgccgtgttt
    30721 gaatcggcgg aagttctcta cgggggcgct cgaaatgggc tacaacttac cgaccaaatc
    30781 aagacctggt gtcagcgtgg gctgcccggg ttgccagttg gccggacagg cttccccctc
    30841 gccttcacgc actcgaatgg cggcctgaag tttgcgcaag gtttctccga tatatcggcc
    30901 gatactgttg tcatgtactt cgtacgcgcg cagcacgcca tcgggatcga tgaggaaggt
    30961 tcctcgcagg gccacaccct cctcctcaag atagacgcca aactcccggc tcaacgctcc
    31021 ggtcgagtcc gcaatcaatg gaaattcaac agtgcgtacc atcggcgaac tgtcgtgcca
    31081 ggccttatgc acatacacgg aatcggtgct gacgccgcac acttccgcac cgagtgtgac
    31141 gaattcagga tacagtcgcg ccagttcggc aagctcggtc gggcacacga acgtgaagtc
    31201 tccagggtaa aacatgagga ccaaccattt gcctcgcaat tcttccaaca cgagagttcg
    31261 tatctccccc tggtgatagg cttgcacggt cgatggcgaa atttctcgtc caatagcgat
    31321 catgatgtcc tttcctcgaa ccgtgtttcc ggtggtcctt cgtgcggacc tacaacgccc
    31381 gtaaaaatgc taccagggca gccttctcat cgttgttcaa ctgcagcccc gccacgacat
    31441 taaagaactc cacgctatcc tccagcgtca ggagcctccc gtcgtgaaaa tatggcggcg
    31501 aatctttgat ccctcgcaac gcgaaggtct tgatgggacc atccgcttcg gcgccgaagc
    31561 gctctaagaa gagatcgtgc atcatgtgat cggtataggc tggaggttgg tgacagactg
    31621 cgcaacgggc tttgccaaag aacagatcct ctcctgcaat ttcctgccgg tcggcgcggg
    31681 atcgatccaa ccgtccggtc atgggatcca gctttggtgc cggtggaaag tcgatcatgt
    31741 tttggatctg tgccatgtgg gccacttgca agcgatcgat gatgctgatg cccttcttgg
    31801 cggcacgaat gtgatcgccg ttgaaatagg ctgtacgctg ttcgaattct gtaaaatctt
    31861 ccacagagcg gagactgcgc ttggagccat gtatctgctg attgaacatt cctcgcaggc
    31921 tcaccgtatc gagacgcaga cggtcacgct gcgggcgggt atcgggattc aaatgaaatt
    31981 ggcccgtcgt atgaaaattc acgtgacagt ctaagcaagc gacaccggcg ctaggcttgg
    32041 gcgatttgcg atctgcggtc aggttgaatt cctcctgcgg aaattgcgtg accagcattc
    32101 tgaggccatc caattgcacg ggcgtgatca acccacgaaa caggcgatca aaattctcca
    32161 cgctcaagac ttcgccctgc gacacatcac ctaattcagg atgggtggta agaaaaatcg
    32221 gcggcggaaa ctccggcaga aagcaagcag gcagatctaa atcgacatcg aaccgctcca
    32281 gcctagggaa ctgatggatc tgttcctcgg gaaacaccat gcctcccacc tcgtgcttca
    32341 cgtgaggcaa tcgcatgaaa ccggcgggaa acgccttttg ctttcggatt tcttccggtt
    32401 ccatctggga cagggattcc cacgtgaggc cattcttgag ttggacggtg gggccgatcg
    32461 gctgtggctt ccctttggtc atgcgtacgg tggaatgtgt tttacactcc agtcgataac
    32521 ggtcctgtaa gagtctcgcc tgttgggcca tgaccttggg tttctcttcc tgatccttct
    32581 tcatggcttc ctgagccagg cgagcggatg attgcccggg aggtccgcca aagaggtcgt
    32641 acgagaaatc cacaccttcg aatccctttt ccttggtctt ctcaatggcc ggattcactg
    32701 tgtgccgcgg ttcttttccg tcccgatcgg catagacata tgccacagac atagccagca
    32761 ccactccaac cgacaccagg tgcgatacag cttttcccgc cgtccgtgtt gtttttcgtc
    32821 tcacagtgtc acctcgtcat aggggccaaa tcgccgcata cgctcgcggt tctgtttgaa
    32881 acaatctgca caggaccgga acggttgtcc tactaggcag acccctgggc cggagccgtt
    32941 cttccctatg acccagtgaa accagggagt ctcctagtgc tccgcccgtc atctgatttc
    33001 taggatggga gtcgatacca aacagaacag atcttgcgtg gaaaggaggc ctactatgaa
    33061 ttgtcatcga tgcagcaact tgatgtttcc cgttgcgctt caggattggg gcggaggact
    33121 catgaatcag gatgtcgcgg cctggcgctg tttcgcctgc ggagaaattg tggatcagct
    33181 gattgcgcag aatcgtaccc gcggtggcga agatgcggaa gagtggcgcc gagggggcgc
    33241 acgccggcgg gtcaacggaa tcggactctt acgttagacg acaatacctc ccatttattc
    33301 cgggcgccgg tgagcaacat cggcgccctg cttatccttc ctctcccgcc ggctcatcca
    33361 ggcgcagtca atcatggact attcggggtg ggatcatagc cctcaaacgg ctgccgtccc
    33421 accaccagca ccaccgcaac cagcgctaga gcgatgagca gataaaaccc gaagcgaaac
    33481 gcttcggtat agggtgtgct gaacgttccg aacgcataga gacctgtgag aatcatgcct
    33541 atcagcacga cggcaaccag tagggtcgtc attgcagcac ctccagcgta agtggtgagt
    33601 ttcccctcag ccgacgcttg catcggcgga tcatcaaccg gtggatcttg gtgcggtgga
    33661 ggatggtccg gtggttcttt ctccggagga tgccgtggcc ctggttcctt caccggcggt
    33721 tttgacccgg tctgcgtcat agggtcactt ccagcaatca gcaacaccca tgcccccatg
    33781 cttctccctg agcatgagcg atcattgcag atttccttat caccccgagc ccggtacccc
    33841 cgcaatcgcg gatggcctgt cctccaaagg acagattgtc tcccccggcc attgcagaca
    33901 caccttattg ctgacacgca aaacaccaga aacctcgcct ccactgcatt ttttgaccag
    33961 cgtccgtttc aagcacggca acggtccgtc ttttgctaac tacggcatca tcggccgacc
    34021 tgcagtgaac gacatacgaa ggagtcacca tgaccgtaca catgaatcgc tttctgccac
    34081 atcagggcct tcatttggca accgctgtcg gtatttccat gatgctcagc ggggtaccgg
    34141 ttgccatgac gtccgcccat gccgccgagg aaaccaaaac aggtgatacc tcacagcttc
    34201 gtgaggatgc acaaacgtta ctcaaaggaa atcgcactgt gttggcaaca gtacttgccg
    34261 tcacctcgga tcagatcaaa gtcgacatcg gtgaagttca accgcgtttt ctcccgctga
    34321 agcaggcaca acagaaaggg ttcccgaaag tatccgaagg cgatgacctc atcgtcgtcc
    34381 tgaacgaaca aaatcttctg gtcgactatc atcctctaga cggaggcacg agcgcccaca
    34441 ccatcatccg tggggaactt tcgcagactc ttccgaccgg gcatgaaacg gctgtcataa
    34501 agtcgggggg caaagaacaa tcgttcaaaa tccgctctca ggcgcggagt aaagtcgcgg
    34561 ccatcccccg cggagccacg gcggtttttc tgatcgacga gacgaatcag attgccgacg
    34621 tcagcctgtc ccgccaacag gcggcccaac tcgcgggagc gcattctcaa ccgattaaag
    34681 gccagcatca acaggttgaa ggaaccgtca cgtctccctt gcagtcgaat cacattacga
    34741 ttcgcactcc cggcggcacg gataagccat acgaagttag agacatcctc cagtcaaaaa
    34801 tcgcgacact ccacaaagga gatgcggtcg tgctgctgat cgatggcgac aataaagtca
    34861 ttgacgtggc cattccgccc aaagggaaat aacgcgcacg gcgctgtggt ctgggacaag
    34921 tccgtcacgc gactccataa ccggctgcga ggccgtaatg gcgaagtttc cgcagcagtg
    34981 ttttccgatg aataccaaga atcgtggcgg atcgtcccag atcccgcgca tgggaatcca
    35041 acacttgaag aatgtggttt cgctcgagat cttcgagggt gacccaccct tgcgcgcgtg
    35101 ccaacccttc aggctgcgcg tccttgaccg ccggaggaag gtcttcgggg cagatgacgg
    35161 aataggggct caacgtgaca gctcgctcga tcgcgtgttc cagttcccgg acgttgccgg
    35221 gccaccagta gcggcggagc agggccagcg cctcagcgga caattccgtg acgggtggca
    35281 ttttcatcga cccgtacagg cgcaaaaagc gtttggccag caatggtagg tcgtcaaggc
    35341 gctctcgcaa tggcgggatg tcgatcgtca cgacattcat tcggtaatag agatcctctc
    35401 ggaatgtgcc cgcccgtacg agaggctcca gcggaagctt agaggccgca atgattcgga
    35461 catctaacgc cgtcgttgcg gtacttccga cacggcgaat ttccttctcc tggaggacac
    35521 gcagcaactt gccttgtaat gccggcgaca ggtcggccac ctcgtcgagg aaacaggttc
    35581 cggaatgggc ttgctccagc agcccctttc gggaagagat cgccccggtg aaggagccac
    35641 gttcgtgccc gaacagctct gactccagca agctgtcggc caacgctcct ccatcaacgg
    35701 tgacgaacgg cccggagctt cgaggtccgc tcgcatggat ggcccgggcg atcagttcct
    35761 tgcctgtgcc gctttcgccc tgtagcaaca cggagctgtc cgtttgagcc acccgggcga
    35821 tcgctttgta gacctgcacc atggtggggc tgctaccaac gaggctggcg aagggggacg
    35881 tgtcgtcagg agcggcttcg cccgatgcag cctgctcggc ccgcatatga gtgtgttcga
    35941 ccgcacgtcg ggcgacagaa cggacttcct cggccgtgaa cggtttactc atatagtcaa
    36001 aggcgccggc tttgagtcct tctaccgctg tccgtagcga accatgagcg gtcatgagca
    36061 cgatcggggt ggtgggacag agtccgcgca attgacgcag gcaggtcagc tcctccgttt
    36121 caggaggaag gtcagtgaga atgagatcga cgggaatccg ttcgaggtag cctccggcgt
    36181 ccttcgggtc gcgaacggcg gaaacctcat atccgtcgac ggacaacatg tctcgtaaaa
    36241 cagtcgcagc gcctacgttg ctatccacga tgagaatacg ggcctgtgtc atggtggtta
    36301 tcatcctagc gaggtgatgg tccaatgcac atgagagtca tagcctacgc tcgcggacaa
    36361 tcgcccgaca cctggatgaa accctggtct ttgggtgtct ttatgatgcg cggcaacgct
    36421 cgttctgacg ccgaacacac cttctctacc cccgctcagc cccatcagga gaagatgtgt
    36481 gcacttgctc gcacagtacc atggtccatc ctcctaaccg tcctctgctg tatggtgatg
    36541 ttttctatgg tgatagtccc ccttctctgt ctttgcccag ccgttccaaa agccagaacc
    36601 cattccgccg gctcccatcc ccggcccttc ccctaaaccg ctgccgcccg cgccaatccc
    36661 tgctcccgcg ccgaagccca ttccccccgc tccaccgagg tgacgcacag ttccctttcc
    36721 tagagagtcc agcgtcttgc gcgacagaaa cagggggttc tcctcggacc gatggagtaa
    36781 cccgccgtga tacgcatgac tagagactca ctccttccac cgttcatgaa gggagcggtc
    36841 atgaagagat ggctggcttg ctgcgcatca gtgatcgtga cggctatctg gttcccctgt
    36901 tcgtccgggg cactcgagtt cacggccgat cgggtgacac acaccgatcg cggatttcac
    36961 cgagcgcgga ttttctatca tgacggcatg tggcggctgg agcacaacga gcgagggccg
    37021 gtgagcatca cgatcgtcag actggatcga aatcaggtgt ggcatctgtt gccggtgacc
    37081 catcattaca aaaccgtctc gtacgatcgc gattatgccc ttcacctggc gacgacgctt
    37141 gatcatgaaa tctcgcggga aacgatcggc gtccagatct tagacggcca tccgactgtc
    37201 ttgtatgagg tcctgaccat cacgccggga ggcggaagag aaagttatta ccaatggatt
    37261 gcgacagaca tcaatttccc gctgaaactg gccaagaaaa atggcgactg gatggtggag
    37321 tatcgtcacg tcaagttcgg gcctatctcg gacgtgtttt ttgagctgcc acgccactac
    37381 gcgcctatgg acgacgatca gcacaccgcg agttcgccgg agccagtaag gaggatgagc
    37441 ccatgacgac aaggacctcc cgacatcaga tgaccggggc aagagaccat atcgtgatcg
    37501 gactgctgcc gaacatgggc gcggtgcgag aagcgctgga agctctgcgg gctcgtggca
    37561 tctccgatga tcagatcggg ctcgcgatgc gccaagctga agaacggtta tccgacgagg
    37621 agagtccctc gcctacagca caggacgcga cgaccgggat ggtcggcgga ggccttatcg
    37681 gcggactggc aggtctgttg accacgaccg gcgtggttgc cattcccggt ctggcaccat
    37741 tgctggccgg cggctcactc atcgggctgc tgggcgcaac gggcgcatcc atcgtggcag
    37801 ctagcggact cggcgcagtc gccggaggac tcgttggcgc actgatcagt atcaacgtgc
    37861 ctgaaacggc cgccaaacat tatgcggatg cggtccacga gggacagatt ctcatcagcg
    37921 tgaagaccga cggcgaccct gcccaggttg aagagctcct cgcttctttg ggagccacca
    37981 cggaccggca atcgccctag cgctggtcgc tgatctttct agggaaacca ccagcttcgc
    38041 cgtcatggga cttcgaccat gatgtcctca gcacgcactg aggaaacctt tccaactttt
    38101 acagggagca ctgcccaatc ggcactatgg ctaagcaaca agcatcccgt acgaagagca
    38161 acacacctgc tgcgcagaaa gttgtctctc agaacggatc ggccgagcca ccggttccac
    38221 accacgaacc catcaaaacg ctcgtcgtcg atatcggggg cacaggcatt aaatccatga
    38281 tcgtcaatga agtcggagag cccattgggg aacggttgcg gctcccgact ccgaaacgaa
    38341 aaactccgcc cagcgtgctg aagaccattc atcgtctcgc caaactgtcc gggcgtttcc
    38401 atcgggtcag cgtcggattc cccggcacca tcagaaacgg cgtcatcctt gacgcggtga
    38461 acctcgaccc ttcctggaag cacttcaatc tcatcgccgc gctcggcaaa accctgcgca
    38521 agaagcccgt tcgagcggcc aacgatgctg acgtgcaggg gtatggcgcg atttccggaa
    38581 agggagttga gctggtttta actttgggaa cgggcgtcgg gtcctccctg ttcatcgatg
    38641 gccgcctcgt cccgaatttg gaagtcggaa agaatcggct gcgaggctcc gagctcgaac
    38701 gcatcggcaa aaaattgtgg aacaatcgct tgctgaaggt catcgccaag cttgaaaaaa
    38761 tgtttcacta cgaccgtctc tacatcggtg gtggcaatgc cgtgcaggtg gatatcgggc
    38821 gggtccctgg caatgtcacc gtcgtctcga acctgaatgg actgctgggt ggaatcgcct
    38881 tgtgggaaga agccgcccac ttcgctgcga gcagacagga actggcgaat aacaacgcca
    38941 accattctgc cccgcctaag ccggcagctg aacgcgccgc accgcgtggg cgctgagcag
    39001 attgctccag cgagccccgc gactgagaca agttgtcctt gctcagacaa tcggtccgta
    39061 ccctgtatcg acctgcgttg ggcatcagtc gtgaagatct ttcaaacagc gctgccagaa
    39121 agcacagtcc acacgtttca taagcccatg agaagaccct gctgtggtcc cgcatttgca
    39181 ccggtgttgt gttgaagaat gtcatacagg catccgtttg gtctcttaac aagaatgatt
    39241 tggaggatca catggcacac atgcgcgggg gagtcagaac agcgccatct gagcgccgtc
    39301 acaagtcaga gcaggacagg aacggcggcg gccggatgga ggcaaggccg catggccgac
    39361 aagatgagac ggaccagaac gtgggtgaca aggaacggtt gatctccggc ctcgtcgggg
    39421 gcggcctgct gctacacagc ctgagaggtc cgttttccgg cctgagcgcg ctcaaagccc
    39481 tcctcggcct cgcactcgtg cagcgctccg tcaccggtca ctgtgccggg tatcacatgc
    39541 tcgggatgaa cacgcatgac cagacggata catcatcgat aggacgctcc aaagtgcata
    39601 gcgaccgggc cattaaaatt gagcgctcca tcacgatcca acgatctgcc aaggagctct
    39661 acgccttctg gcggcaactc gacaatcttc ccaccatcat gagtcaggtc cgcacggtcg
    39721 aacctctgga tgatcgcagg tcacattggg tcatcaacac cctgcccggc gctcctaccg
    39781 tggaatggga tgcggaaatc atcaatgagg tcgaaaacga gcgcatcgga tggaaaacgc
    39841 tcgatggcgc aacggtcgaa cacgcggggt cggttcggtt caatccgttg aacgatgaac
    39901 gccagacccg ggtaactgtc acattgcaat atgatccgcc tgccggggcc atcggtgccg
    39961 ccatcgcaga tctgtttgga caggagccag ggcagaagat cgcccgcgat ctcgagcgtt
    40021 tcaaagaagc gatggagtca cacaccaccg tctctcagtg agcagacagg agtgcgcggt
    40081 tcgattccta ttgcgcatgc gttcggtctg ccggagtgaa agggggaagt atgaatatgt
    40141 ggcgaggcct cacggtcgca gtgatggtga tgggagcgac tgtcatttct ggagtgccgg
    40201 gtggagccgc tcaaaaggaa tccatggaga agacgccgaa ggtggaagac gacacatcgg
    40261 acaaatctgc ggggaaggcg tccaaagata aggtcatcaa gaaacggcgg tcatcaggca
    40321 agagcaatgc cgaagacaca accgatgcca aatcacaacc caagtctcaa gagtcgcgtg
    40381 aggggacacc cggcacgacc ttgatgttca ggtccgacgg cgcaggaaac tccggcgcag
    40441 cgggcaaata gacaggatca gcggagaact tttgcatcca taatcttgaa ggaggcacac
    40501 catgccgaaa cagaacaggc acatccggct gttcgccgcc atgctcggac taggaatcat
    40561 gagcgccaca ggggtctatg cggaccaggc acgtgaacag agagaagcgg gcctgcaggg
    40621 gcagagcgat gggcgagtcc acaatgcaga tatcaccggc ggcttctatg acgaccgcta
    40681 caaactcgac gactggtact acgattttta tgacagtccg ggatattccc gtgctgattc
    40741 cacgtccgtc atccaagcct ccaacgagaa tacggccacc atggcagatg ccgctcatcc
    40801 atccttgcgg catcatcggc cctaccgcac tgaccagaat gtcccggcca cgccctatgc
    40861 cggagatccc tggttctatg cacagcggga ccccatgtac ggcataccgg aaaccagctc
    40921 ggatgcgtac ggtggaaacg tcatcaaagg cacgatcaag gcggccaaac aagtccgcaa
    40981 ccggacaacc ggtgatcaaa ataccgtggt attgctcacc actccggatc gccggcaagt
    41041 caccgctgac cttgggtctt cacgacgcac catggatctc gcactgacga aaggtgattc
    41101 gctcgtcgtg gccggtccat gggaggatgt gggtccttac tccgtgctta tggcgcagca
    41161 gatcaggacc gaagccaagc gcgtcaccct gaaccgtccg atcggggccg cccgcggaga
    41221 caggaccgtg gaaggtcgta ttcagcaatt tcgagacatt cgaatgaatc gcagcgggga
    41281 actccatcgc gcagcggctg tgcaaacccc ggatgggcgg atggcacttg tggaccttgg
    41341 gccggataat caggaccgga cacccttgcg agctgccgcc ggcgaccgga tggtggccca
    41401 ggggccggtg gttcaggtgg gcaactaccc tgtcctctgg gccaaccgcg tggccatcaa
    41461 tgacggcatg ccggttgacg tgacgcgtac gactgcgccg actgacttcg taccggcaac
    41521 cgggaacagg caggaaaact gtctcggtgc cggctgcgac aaccagccgt taaatcaggg
    41581 ccagcccggc aaccggaaca gcaacgcgat ggatggcacc actcgctgat ccgggggact
    41641 ccctcctgcg cagggaaggg ggacccattg gatcctcctt ccccattttc acgccgctcg
    41701 tgctgctggt gtcgatcatc agataggggc caggcccggc cctcgggtct cgaaacgttg
    41761 tggggtacgg ggggatgtgc cgatgcgggg cgcgcacggc gagttgtcgt tgttgattaa
    41821 caacaagggt gtcgcgacca cactgtttac aaacgaatcg tgatgctgct tcgcccggaa
    41881 acggggtcca agctcgtcac catctctctc accttgcgct ccaccaccgg ctcgattcgc
    41941 tcacgaagaa tccgttcaca ggaaagaaac atcgcctcgt gaacctgccg taacaatgag
    42001 cgtccttccg gtgattcggc caatcgcctc tcagccggta ccgatccctg cctcgtcaac
    42061 gtcacttcga tttcatcctg agacagatgc acctggacat tggcatagtt ggatcgcatg
    42121 aactcagtgt gaaagttcag gatcgtcatc atgagcgcat actcgagatc ggctttattg
    42181 gtgcttcttt gtgccatgct ctgctccccg atagtctgaa gacgaatcgc aacctgccga
    42241 tgccgcttac gtgcggaggc tgtcaaaccg gacagtgacc actcgcttca cactgtacca
    42301 tgaaatcgca tcccggccga tgcacggtga aacgacacgc gtgggacatg ccggcctatg
    42361 aagtcgccag acatgatgaa gcctcggggc acatggggcc ccgaggcttc cgtgaggcta
    42421 ataggccttg ctgaaggccg gttcaccatc atgagtccag agcttttgcg ctgcggacca
    42481 ccaccagccc ttcttagtca agaccgccag gaatgtatcg acgtcagcat cggtcacatc
    42541 ccctgtcgcc atgaaccgac aacgatacca gttcaccatc atcagatcgg gactgacgaa
    42601 ccccggccag accttggttc cccgattgga gatgaactcg catttaaacg gcccgaactc
    42661 gccgtccgga aatttcggaa tgccttcttc ggtgacgatg tacatgtcca caccgacgag
    42721 cttgataact ttcttcttcc gcgtcgcttt cgctgcttca gaaaacgttg ttgccatggg
    42781 cgcttcccct ttctttatca atggcgggat tctcaggctt tcccctgcat cttgtcgacg
    42841 atggcttgag catattcgtc ggtggtggcg gttccgccta cgtcgtaggt gacatgtttg
    42901 ccttcatgga ggaccgcgaa catggctttt tcgatacgat cagccgccgc agtctccttc
    42961 aaccagcgca gcatcatgat gccggacacc aacaccgccg aagggttcac ctttttcatc
    43021 cccgcatact tcggcgctga cccgtgcacc gcctcgaaga tcgaacagtt gtccccgata
    43081 ttggcgcccg gcgcgaaacc caaccctccc accagccccg cgcagagatc cgtcagaata
    43141 tccccgtaca agtttggcag taccagacag tcgaactgag cgggattcct cacgagctgc
    43201 atgcagcagt tgtctacgat gacgtccccg gtttcgattt caggatactt cttcgcggcc
    43261 tcgcgaaacg catccagaaa cagcccgtcc gtcattttca taatgttggc tttgtgcaca
    43321 cagtacacct tcttccgacc attggctctc gcccactgaa acgcaaagtc ggcaatgcgg
    43381 taagagcctg gccaggtgat gaccttcagg cattgcgcca cttcgtcgga caccatatgc
    43441 tcgatggccg cataggaatc ttccgtgttt tcccggaagt taatgatgtc gatcttgtcc
    43501 caggggcgct tcaacaccgg aatcagctta gccggccgga cattggcaaa caggtcgaac
    43561 accttacgca acgtcacgtt cgcgcttttg tgccccgtcc cgacgggcgt cgtcgtcggg
    43621 cctttaagcg ccaccttgtt tttggcaatg ttcttgatgg tcttatccgg aagcaaggtg
    43681 ccgtacttct ccaaacatcc caatccgatc tcttcgtatt cccatttgat atcgacaccg
    43741 ctcgcgtcaa tcaccagtcg caccgcttcg cagatttcag ggccggtccc ttctcccgga
    43801 agcatcgtga ctacatgttg tgccatcaga gaacctcctt gcgtcaccag ggggtaaccg
    43861 gaccatccgt tcccccgctc gcacagacat catgtgcaaa ggcgattcca cggcgtagtc
    43921 ttgcccgtgc ggcggtgtct gccgcatttt tcagtacaat tcccgtctcc ggcgtccgtg
    43981 caggtcggtc ccttggtatg ggaacggtgc gatcgtgcac agacacacac tgtacgctgt
    44041 gtgcctgtgc atctgtgctc agcaggataa gcggacacta cggttggaca agtgcctggg
    44101 cgggaaacgt ttttgccaga ggccggcagt aaccggccag ttggggagat ccagtcacgg
    44161 ccaccacccc atggggcccg gcagcctgga ccgcacgcct gaatgcggct tccgtactgt
    44221 ctaccacctc agcggcgacc gaagagcagg ttcgccattg ttcggcaatc gcgtcgggcg
    44281 gcacggccct atcttgtgga ccgccggtca cgatgagccg gtcggctccc gctccggcaa
    44341 gagcgcgcaa cacctgttcc cattgtgcat cccgcgccag ggatacgagc aagacactgc
    44401 ggcggccctc gaaggtcgtg cggagaatag tcgcgagccg cattgccgcc ggggcgttgt
    44461 gcgcgccatc cagaagcacc gcgggcgtgc cggacagcac ttcacagcag gctggccatt
    44521 ccgtcttctc gaatacccgg ccgatctcgt ccggatcgat gtccggaagc agtcctcgct
    44581 tgcgtaacac gtcataagcc atgagggcat gagcggcatt ggcggccatg aatgccgcag
    44641 gcgaccgtag tcgcagctgt ggatgcgtgg acgcgccgac ccttaccgta caaagtcccg
    44701 catagccgtc acggatgaac tcggtgactt cataatcctg cccgctgagt agcacgggtg
    44761 ccttgagcgc cgcggctcga gacaggacca cggcccgcgg cccctccggc atcgggccgc
    44821 acagcaccgg cgtaaagggt ttgatgatgc ccgccttttg gccggcaatc tgctcgagcg
    44881 tgtccccgag catgtctgcg tggtcatatt cgacggtcgt aatgaccgcg agctcaggcg
    44941 cgatgacgtt ggtggcatcc tgccgcccgc cgatgccgac ctcgatcacc gcatagtcca
    45001 ctttggccgc cctgaacgcg aaaaaggccg ccgcggtcag gaattcgaac ggagccagtt
    45061 gaaggccgtg cacgtccaga aacgccttga cgtggttaca ggcatcgacc agctcatctt
    45121 cgccgatggg ctcaccgttg atcgcaatgc gttcccggaa attcgtgaca tgcgggctcg
    45181 tatagacgcc caccgtcaat ccggtcgccc gtatcagcgc accgatcatg cggctgaccg
    45241 tcgtctttcc attcgtgccg gtcacgtgga tcgagcgata ggccaaatcc ggccgcttga
    45301 gttcatgcag gcagcgagcg accgctccca ggctgacggt gccgtcggac ggctgccccc
    45361 gcaggatgca atcgacttca acgcctcgca aaaattcggt cacttcggca taggacgaga
    45421 acggcacggt ccaagacgga ggacaggtct tcgttcgtct accccgcata cacacctccc
    45481 tggctgggga cagttcaatg ccgctgatca ggcctgcggg actggcgctt cacccacctg
    45541 ttcagccata tccgcgacga caaaccattt gccgccatca taacggtaat atttggcctc
    45601 ggccggctcg caggcgatga gtagcagcca ccggttgttg aacagtttct ccaggagcgg
    45661 ctgccgttcg atgatggcgc tgaggcgttc cctcggcgcc tcgatgatgg ccgtgaggcg
    45721 catcggctcg tggtaagggg cctctccacg catgaccgtc tgagtcggca agcccatccg
    45781 aaggtcgctc tgactgcccg tcatcacgcc gatcccgccg gtcacgttat gataaatctt
    45841 gctcccgctg ccgaagacct cgggggatac ggcggaaaaa tagtattcca tattgatcca
    45901 ctgcgcgacg atcaagggcg cggtcatgat gatttcgagc agcttgccgt caccatccat
    45961 ggcatggtcg tatgagtgca ggaacgagcg gccgttcaga tcggcctccc gcgtcaatcg
    46021 cctgctgcct acgataaaga cactattgcg cgacaagccc cattcgggac gcacctgcgc
    46081 ccaatccacg ctgcgccgtt cgagttcctg ccgagccgcg cgcgttccct catgcgattc
    46141 ggcggccgtc aaggccaccc agcgttcggc cgccgcttcc ccgccggcct catgaacatc
    46201 gtccaggatt tgggcaagtt cctttcgatg agttgagggg acatcttcga gatcggcgac
    46261 ctgaacccgg tcggtcgtcg tatcgtggag ggcggcgaca aactgtgtat ccggagggat
    46321 cgtcaaccct ctcgaggcca aaatctcgcg aacctgcggg cgattggcca tcatggcaaa
    46381 tgcgcgcgcg ttgggaagtc cctgtcctcc accgcaagcc ccgcaatcca aagacgactc
    46441 atacggatta ttttgcgagg tgctgccgtg accgcatagc acgaccaatc gagcgaagga
    46501 tccggtgagg cccatcagtc gcagcgatgt ctcgacgtag tacgcctgct cattgacggt
    46561 aaacccggtc cgggtaatgc gatcgagtcg ggagcgagtc cctcgatacg tcagccggca
    46621 ttcgcggcgc agcgtgacca gtgcatccgt ttcctctgtg gagcagacac ccaagagccg
    46681 gccgagggcc ccgggatgcg aggcggattc ctcatcttcc tcgagtgctt gacgccggac
    46741 ctcatccaac acagctcctg tgaccaggct tccagcccgt tggccatgtt tccttaacca
    46801 ccaggcaatc tgcagacgtt gttcggcggc aagcatgttc tccgcgtcgg cggtcgaaaa
    46861 cttatcgacc gtcaaggtgg tggcgaccgg cggcacgacc caggatttga cccaggcggc
    46921 aaatcgatgg taccagcggg ggcagagcgt tttcccgagg aagggcaaca ggaaaaacca
    46981 gccgagtgcc tcgaccatga cgtagggcgt gacgacgttg tgcttcaagt cgtggagcag
    47041 ttcgtggcct gccttgacga gatggctgct gtgcttgcgg cgttcggcca ttgcgtcctg
    47101 atacgtcctc ggcacctccc tgatgaggtg tttcggtttc aacaggaccg ggcagagcgg
    47161 cagttcccgc tgctcgtcca acgaacgata ggagaacggc acgccaaaga accccgcgaa
    47221 tccgtacgtt tcataccccc cgcgattctc gagatgcctc cgaaacacct cggaccgtac
    47281 atcgatgcag aacacgaact gggcgactgg tcgcgccgtc atcgcgcccg gcgcggcgat
    47341 ccgcgcgtcc tcacctgctg gcagctcgag tttgcgcagc acctcgcatt gatgactgcg
    47401 ttcgaatgct tccagccatg ccacacgctg tttagccgga gtaaactccc gcatccatcg
    47461 taacaacata gcgaggtcgc ctggcgaagt agacgtgatc tcgtggaccg gcacggcgag
    47521 cgcgcccgac aggctcacga gattctgggc ttccaccctg taggttcgct caccggcagg
    47581 ctgttcctca aaccaccgcc ggcaatcctg ttcccaggcg gctggatcgt atgaccggcc
    47641 cgctcgctgg aggcgggagg cttcaggcag caaagctgaa ggaagatccc cggacatcga
    47701 agctctcctg aaccagagcg tgtatggata ccgtttccca tactccagca ctgcgctggc
    47761 cgttccctcg caccccagcg aggcacggca cgcggtcgcg acaagctcgc gttcataaaa
    47821 cagccggacg gcaagatact tcacaagatc gatgcgatag accgactgcc agacgtgcgc
    47881 atcctggtct tcccgccact tgataaatcc cgcccagcct ggcaaggcgg ccaggtggcg
    47941 cgacaaatac gattgccaga ttgcgtgggg gatgtggagc atctccagcg actccaacag
    48001 gacgtcttca gggcggtcgg ccagcgccgc aattttcgat gccgcccgtt ccacccccgc
    48061 acacctgaga ctgaaatcgt atcgcgcggc agctttccat gcgcggaaaa atgtctcttc
    48121 ccgcttcggc atgggccacg ccgcctcccc ttcgtcgcaa aacgccgcgc accacttgat
    48181 catctggcgg ttgatttcat ccgtgatggt gacgccgagc gttcgatcgc accagtcggc
    48241 catcgtgctt tcacaaggcc attccccggg gatgtactgc aaacccgccg ccctctccgt
    48301 ctctagtgtc tcgcggggaa gcatgcgccc caaccacgcc gccaggtgag ggacggtcac
    48361 ggagtctcct gtgggcgtag gcggggccgt cgctgctgtg cctacccgat cgtggtcgcg
    48421 gcccactgcc accatgatgg ctctgaggac atccaggtga gtaacctggc gatcgccaaa
    48481 cgtgatctgc cgatcgcccg cctgccctcg caaggcctct tcgaaatgtt tctcttgaat
    48541 gcggcctcga cgaaatgctt cccggaaggc ttcccccgag agggtgcccg atccgccgaa
    48601 cagctgctca gcccgttcga ccgcctcatc gaaacggaga tgctcgaatc cctgcagggg
    48661 gtttcgggcg atcactgacg tcatcggcca caccttggcg atggtctctc cggcgaggct
    48721 cacataagac cgcaactcca ttcgttgtgc gtcgcgatga cgctggtccg aaccattcgt
    48781 tgtatgcatg ccgtccctca ctttgtccct gtgtcacagc aagaacgggg ccaaccgtct
    48841 cccgcaccac cacagatgcg gctggctctg atttgttcgg aaggtcgcga ttcaatatct
    48901 gcctcgcgac gcggcggaag ccggtcggca aggtcgaggc gtgcaagggt acacgaacgt
    48961 gcaaccatgc acgtccgtgc agagcgcaac ccgttgaaca gggaacgtgg gccatcaatg
    49021 cgggtgcata ggaggagcta catatggccg agcttccgga gggtttccat gccgcgtcct
    49081 tcgtcttgtg cgcggccggc tcgttcggct gtcgtcgtgc ggcgcagttc ggtgatgatt
    49141 tgaggtatgg tggaggcgca ggaatcaact gtgccggtgc aggggacaca acctaagctc
    49201 cgatatcgtg tgccggctcc cctgtcgaaa tatgggctcg gcagctcaat tcgttcctgt
    49261 tcgagatatt cccagatgtt cagctcggtc caatcgagaa gaggatgcac gcgaatgtgg
    49321 gaaccgggcg gaaacttcgt ctgaaattgt tcccagagct ccggcggctg gtcgcgaaaa
    49381 ttccactcgc cctccttgcc ccgcaaagag aagtatcgtt ccttggcacg cgtgccctcc
    49441 tcgtccgctc gaatgccgag gataatggcc gaccagccgt gctgggcgat ggtctggtta
    49501 agggcatcga ttttcatggc cgtgcagcac acaacccggc cgcggtccgg tcccatgccg
    49561 tcggccagcg ccgccttgtt ctggcccacg atgagattca acctccagtc gcggcatatg
    49621 cgatcccgat attcgatcaa ttccggcatt tcgtaccccg tatcgatgtg caccagcgga
    49681 aaaggcacgt ggccgaaaaa tgccttgcgg gccagccata acagcaccgt cgaatccttc
    49741 cccatcgacc ataacatcgc caagccgtca aaatgcttat aggcttcgcg caagatataa
    49801 acgctctgat cttcaagctc tcgcaggtgt cgcatagcat ctcccaggca ctagatgaat
    49861 tccatccgcg caggttttcc agtcgttagc ctcacgaccc gctcgtggcc ggatcgacta
    49921 tcaaaccgtc cttgcaatat gaggtcccgt taacaacgac cacccctggc ttcctgcggg
    49981 agggccatat gccggcaggg gacggcagag acactgtccg gcaacgaacg ggtctggaac
    50041 ggatgagtgg atagatgcgc tggttgtgcg cggacagacc ttcccgcgca caggtgtgtg
    50101 cctgcgcacc ggggcgcagc gcgccgtcgc agtcttgtgc ggaagacagg tggggggtgg
    50161 gaatctggac tgctagtatg ccttgcggcg ccgatcgagc atccagcgcg tgatccccag
    50221 gtgctttgcc gccagggttt tgttgccgtc gcttcgttcg aggacggcct gaatgatggc
    50281 atcctcgagg tcgctcagcg accgctcgcc gaccttgaaa gacaggctga ccatgagcgg
    50341 gtcggcttca gcaggggccg ccgtaggctg aacggctccg gggagtccgg ggagtgaggc
    50401 gggcgccacc gccgcagcca ggtccgccgg aaaatgagcc gcatcgagtg acggaccatt
    50461 gcaaaagatc accgcccgtt cgatgagatt gcccagctcc cggacgttcc cggggaagtg
    50521 ataggcttgc agtaattgtc tcgcagactc ggtcagttcg cgaaccggct tcttgagggc
    50581 cagcgccgac cggagaaggc agtgttcggc cagcggaatg atgtcttccg gccgcgctcg
    50641 caagggcgga accgtgagca tgacgacgtt cagccggaaa tagagatctt ctcggaattc
    50701 tcccttcgcg acagccgcct tcagatcgcg gttcgtcgcc gccatgaacc gcacatcgac
    50761 cggcacggta ttcattcctc cgacccgtcg cagcgaccgc tcctcgatca cccgcaacag
    50821 cttggcttgc agcgccaagg gaagatctcc gatctcgtca aaaaagaccg tcccgccttc
    50881 agccatctcg atcagcccgc atttacggcc tgcggcgccc gtgaatgcgc ccttctcatg
    50941 gccgaagagt tcgctctcga agaggtctcg tggaatcgac gggcagtcga cctcgacgca
    51001 gggcttgctg ccacgcgcac tgttgtagtg gatcacccgg cccatgtact gcttgccggt
    51061 tcccgtttca ccttgcaaca gcaccgtgac gcggtcgttg cgcacgagtt cctggacctg
    51121 ggccagaaaa cttttggtga ccggactctt ggcgatcacg cgatccaacg cgtaccgggc
    51181 ggcatcctga ccgctatgca actgcacctg acgctgaagg gtcaagaaat cggtcaccct
    51241 gcgcagggtg tggagcagat cgtccatatc aatggacttg gccacgaaat cgaacgcgcc
    51301 gagccgcatg gcttccaccg catctttgac cgttcctctg gccgtcagca cgatgaccgg
    51361 cagagcgggc gacatctgct tcacccgcgc aagcacatcg aggccggata gattcggcat
    51421 caccaaatcg actatgagga catccggctc gaaggaacgc agcagtccaa gcgcctcctc
    51481 gccggtctcc gccgtctgca ccacatagcc cttgtcccgc aaacgtaatg caaaggcctc
    51541 gcgaaccgac gcctcgtctt ccaacaacaa caaatgccac gccatgtcag gcgcctccat
    51601 atggacggtg gtccgtccgc atcatcgata ggccatcacc gaaaaacgct acgttcagca
    51661 tgctgctacg cctcgtgttt gagaggaagc cacacatcga cgactgctcc cctccccgga
    51721 gggctctgga tcaccagctg gccgtggtgc cgctcaacca tatcgcgggt gatggtcagc
    51781 ccgagcccga cgcccttggc ttttcccttg gtgaagaatg gctcatagat ccgctccaga
    51841 tcgtccgccg gtatgccctt cccggtatcc gcaaatcgca tcaacacccc gggaacccgt
    51901 cgggcttccg gcacaagtgt gacatgcaag tggccgccgc ccggcatcgc atcgatggcg
    51961 ttgagcacga tgttcaagca gacctgcaga atgtgggctt tggaagcatc gacagaggga
    52021 aggtccggcg cgacggaggt cttgatcagg atctgattct tcacgatgtt cggccgcagc
    52081 agagtcaaca tatcgtccaa taccgaagcg aggtcgcagg acgtgatatc aaacgggcgg
    52141 gggcgcaatt ggcccaagtg ggactccagg agttcatcga tccgtgcggc ctcccgcgca
    52201 atcagccggc accggtcgcg cgcttcctga ggaagcgagg cctggtccgc gagatgggtg
    52261 gccagactgc ccaacccgat gaggggattc ctcacctcgt gcagaatgcc tcccgcgagc
    52321 tggccgacca gctttacttg ttccgtgtgg cgcagggctt ccaacatttg ttcgcgttcg
    52381 ctgagatccg tgagaatcgc catgaaatac tgggtcgtcc cgtcggaggc cttgacgggg
    52441 ttgacactct cccaaacgag gaattccgat ccgtctttcc gccgattcac gaaccgttcg
    52501 acaaagggct gcccagcgcg aatggaagcc cacaaacgat catagaattc cggcggatgc
    52561 tttccggatt tcagaatcgc cgggcgctgc cccagagcct cttctctcgt ccatccggtc
    52621 atccgctcca gagccggatt ccattccaga atccggccgg tcgtatcggt catcatgacg
    52681 ccgtcctgtg cggacaggaa caggcgatga tagaagtcca tctgcgtttc ctcatgccac
    52741 cggcgctcga ggaccgtccc gatggtgttg gccatggtgc agagaaactc ctgctcctgc
    52801 tggctgaact tgcgcacccg cttggagtgg gccgtcatga ccccgtagac cctgtcctcg
    52861 actagcatgg gcacacacat gcccgacacg gctccatgtt cggtcaacaa ccgcgaaggc
    52921 gagaagcgcg tctcgcggcg taggtcgtcc acgacaaccg gaagacgctt ggcaatcgca
    52981 tacccggctt gagaatgcgt gccgccctcg atggtcagtt ttccgatcag ttcgctttgc
    53041 aagcctgatc cggccatgat gtacagctga ccatcgtggg cgcgcgggac caggatttta
    53101 cacagctcca cttccagcgc ggcggcggtc tctttcaccg cctcattcat gagctcttga
    53161 gccgacacac cgctgacggc gagcgtgccg atgcgagcca acaccgattg aaaccgggct
    53221 acgtgggacg catcctgtgc gagcataacg cgttcggtca catcctccag caccataagt
    53281 acgccccgct caccacgata gggcacgaca ttgaatcggc agacgtagta gagacgcccg
    53341 ccgtcccggc cttcgagctg gtattccacg ctcttgcgtt cgccaagcgg ccgattgttc
    53401 cacgcaatgg catcgtcgag gagccgtttg cgcagggcat gctcatggcc ctcgaccctc
    53461 ggaagcccgc tctcgaccaa ttccgcaaat tgagaatttt cgaagatgag aatgcctttg
    53521 cagacaaacg atagtcctcc ctggatgcaa tcgctcagcc aggccagtac ttcggccgcg
    53581 gcgatttcag actggactgg ccgggaacgc gtggtggggc tgcggcggga tgacgccatc
    53641 aggtctcgtc tctatcgggt ttggcggatg atttcattca gcgctcacag tcgcgcacgc
    53701 ggaaacgggg cgcctgcgac atcgaaaagc cgctgtgcac cccctagcaa tctagggggt
    53761 gacgaaatgg tctcgacgcg tcacggctac gtcgctgacg aacatcgcac cggaatgttt
    53821 gtgaaacaac gggccaagcc ccgccaggat gggctcgatc cgttcctcag gcacaacggt
    53881 gaagacgatc acctggctgc tcgtatcatt gaacagcaga tgcccctcat ggaatccatg
    53941 atggcctttg cccgacacat tgttgatgat ggtatacccg gtcgcgccca cccggtcgag
    54001 caggctggtg acgattttca aatgttcccc ctccacgaca atgcgaatct ctttcatcgg
    54061 atgcagcgtc agcatactcc gtctcccttc ctctatggat tcatcctctt ttgctcgaat
    54121 gccgttgctt cctgaaccga ccccaccgtt tcaccatcct tctggtgcag aaacggcgct
    54181 gattcgcgaa cgaagcgggg agcagcagcg gacgactgct ccggcaagcc ggacaccggc
    54241 tgccagcccc gcatgatgtc ataatgataa aaggcgcctt cgctcgcctc gcacacgacc
    54301 agcaacaccc attcgttgtg gaacaaccgc tccagtcccg gatgggtggc aatgacggat
    54361 tcgacacggg ttctcggcgc ttcaatgatg gtcaacagcc gcatgggctc gtggtagggc
    54421 attgagccgt cgagcacggt ctgcgcggga agtcccaggc gaagatcact attggctcca
    54481 ctcatgacgc ccacccgacc gacgatattg tgatagacct tgctgccgct gccatagacc
    54541 tcgttgtcga cggtggagaa ataatgctcc atgttgatcc actgcgcgac gatgagaggc
    54601 gcggtcatga tggcttccag cagcttcccg gaactgtcct gccggtgatc gtaggaatgg
    54661 agaaaggcgc gcccctgcaa attcagcggc cgggtgagtt cacgccgccc gacgatgagc
    54721 aggttgttcc tcgacaagcc ccattccggg cgcacctgag cccagtccac gcttcgtctg
    54781 gccgctttgg tatggggatc ctttcgcgac cacgtggcga cgggtccccc taacgccagg
    54841 ccccgttcct gtgccgcctg ggcgcccgct tcgcggagat cctccaggag ccgtaccagg
    54901 tctttccgat gagtcgcagg aatatcttcc atgtccacga tccggacatc gttccgcgtc
    54961 gtatcatgaa gcccagccag gaaatgcgta tcgccgggga ttctgatgcc ccgcgtttcc
    55021 aataccttcc ggacggccgc attatttgcc atcgacgcaa aggcacgcgc attgggcagg
    55081 ccgctgttgc ctccgcacgc gccgcaatcc aaggcggatt cgtaggggtt attgtccgag
    55141 gtgctcccat gggaacagat caccacgaga cgcgaaaatc ccgacgtgaa tcccatgaga
    55201 cgaagggccg cctcgacgcc gtacgcctgt tcggagacgg aaaagccatg ctgggtgatc
    55261 cgctggagtc gcgacgacat accacgcggc gtgaggttga aatcgcgccg gaatcgctcg
    55321 tacagcgcag cgacttccac aggggagatg ccgaggatgt gcgccacgtc cccgcccctt
    55381 tgtccttccg catcttccat cacgccgaca cggatcgcat cgatcagcgc cggggtgatg
    55441 gcggaaccga gggcaggaaa ttcctgccgc aacgccgcac gaatgaccgc gcgctgctcc
    55501 accgcaacca tctcctccgc ctcttctctc gtgagcttat cgatcgtgag cgtggtggcc
    55561 aggggcggaa cccacaggct ctttaacgaa gacgtgagac gttggtaggc aagggggcac
    55621 agggttttgc cgaaaaacgg cagactgaaa aaccatccca gtgcttccac catgacatag
    55681 ggggtaatga cgttttcttt cagatcgtgc aggagcgtat gcgccacgtg gctgagctgg
    55741 gcaaacaacc gatgtcgact tgccgccgcg ccatggtaac tgcgtggaat ctcgcggatg
    55801 tggttcttgg gttttaacaa caccggcgca tgggtgaccg gatgctcttc tccgaacgcc
    55861 tgatacttca ccggtatgcc gaagaacccc gccaccccaa aagtctcata acctcccaac
    55921 tgctccagat gccttcggaa cacctcggaa cggacgtcga tacaaaacac catttgcgcg
    55981 agcgggcgcg ccgatccggc tggtgcagct cgatcatcgg tggcgcgaag ttgtttcgtc
    56041 acggcccgca attcgtcaac tacgtcgcgg cgatgcttgg cttcgagggc ttccagccaa
    56101 cgctggcctt gttggccggc gggaaatcca tccagccaat tcaacacggt cgccgcatcc
    56161 gccactgagg tctgactgat cagcgccgga tcagtgccga gcgccgcggc cagagcaagc
    56221 aaccggcggg cgtcccgctt catcgcttca gtgccacgcc actcggacgt ttgctcatag
    56281 agacggctgc cgacctggtt ccattcatcg agcgtggcag cctggtggga ccgacgccac
    56341 tcttcggctt cccggatcgc ccatggtggg atggttctgg ccgtcagctg gcgccgtaac
    56401 caatgggcat agggggcctg atcaaggatg gcgcgaatgg catcgtactg tcccggccga
    56461 tttaacgcgc gcctgcagtg atactcgaca aattcgcgct cgtaaaagag ccggaccgcc
    56521 aagtacttca cgaggtcgat cgggtaccgc acctgccacg gatggtgctg ctcctccgct
    56581 ctccatttga tatatcctgc ccagccaggc aaggcggaga ggtgcaaggc gaagtaggct
    56641 tcccaggcag acttcggtat tcccagatcc accaggcaag agaggaccgc ttcttccgga
    56701 cgctcgccta aagcatcaat ctttgtagcc gcatcctgaa tccccagcag ccccaagccg
    56761 gcatcgtaac gggccaagcg tttccaggcg cgataaaacg tctgctcccg gttgggcatg
    56821 acccaggacg cttcgccttc gtccagaaag acgctgcacc acttgatcat ctgctcattg
    56881 atgtccgaca cgatgcaggt tcccaaggtc tggtcgcacc aggccgagag cgtctcatgg
    56941 gacaagaggt caagcgactc ccagggcacc aatggctccg gctgacgaat ccgcgtcccg
    57001 gccacggcgg taccgagcca ggccagtaga tcatcgagag cggtatcccc ggccgcccca
    57061 gagtcctgca gtttcgccgg taaatcgacc aggccatgca ccatagaaac gcgcatcagc
    57121 tccagatgtg tcaattcgcg ccccgcaaag gtcacgcacc ggtcgctggc cagaggcgcc
    57181 agcacgcagg acacgtcatt caggccgatg cggccttgct cgaagaaacg gcggtagatg
    57241 gcattcgaca gatagccctt tccacccaac aaatgctctc cgcgtttgat ggcctggggg
    57301 aaaggcatat cctccattcc atgcaacggg ttatggtgaa tgaaggtccg catgggccag
    57361 tactgggcaa tcgcttctcc cgccagctcg acatgcgagc gcaattccat gcgctgggct
    57421 tctgtgaagg cacgtgtttc aatggccatt cagtgactcc gacttcgatt ccattgcggg
    57481 gcatctcacc ggcgcgatcc cgcgcggccg atcatgcgca ccatgtcctc gatatagagg
    57541 ccgttcagga aggcgacgta ggcacgagtg cgcaaggact ccatccaccc cggcatgagt
    57601 actttgatgc ccttcgcttt tccgtagatc agcccccagg ctccgacgat catgatagcg
    57661 gagactccca ccaccgcgtc gaacagggca cgattccatt ccgcagcctg cacgtacgac
    57721 gcggcaatgc cgtgagcggg atagaggaaa tgcgtgaagg cttcaccggc ccagagatac
    57781 gtcaaaccga tgagagccag cgcgccgagc atggtcaggg agacattcca agacgctccg
    57841 gcgtgcaggc gatacaggct gaacatggcc tgcgaggtcg tcacccaacc gaagaacagg
    57901 aaaatgaccg taccttgcga ctcctgcaaa ggaatactca cgacgccatg cgccaccagc
    57961 agaataatca acggcaacac cagcgtcacg gcgaggcctg tggtccaggt catcggtgaa
    58021 aaggcgcggc tctcctccac ccggttgcca ggaggcagtt tgaattccgt cctggcctta
    58081 tgaatctgcg cgccggagtt gagaaacagc gtcgctttga acaggccatg ggcgcagaga
    58141 tggaacactg ccagcgcgaa ggcccctagt ccacattcca tgaccatata gcccatctgc
    58201 cccatcgtgg aatagaccag tgtgcgcttg acgctggact gggtcagcat ggcgctcgcg
    58261 ccgattaacg ccgtcaggcc gccgatgacg aacaacaggt gaagcgtggt gggagcgaga
    58321 ccgaacagcg gcgccattcg gttgaccaga aatccccccg cgtttacgat tccggcatgc
    58381 atcagtgctg atacgggcgt gggcgcttcg atggtgccga tcaaccagaa gtggaacgga
    58441 aattgtgccg acttggtcat gaccgacaga accaggagga gcgtcgtgac aagagggacg
    58501 ttcagttcga gcgcgagacc ggaccagctc gtcaccggtg ccgcctgcac tcgcggaaac
    58561 agctcggtca cgtccaacgt gccatacgaa ccatagagga tgagcaagac cagggcaaag
    58621 gcggcgtcac ccagactttg cacccagaac gtcttctgcc cggcgtccct cgccgcgagg
    58681 ctcgccgggt tgcacaccaa caaggtcgcg agcaaccacg tcaccagatg ccagcaggca
    58741 aacatccata acagattgcc gctgctgacg aggcttaata acacaaacgt gaggctgccg
    58801 agcagagcga agaagcgggt ataccccgca tcgccgacca tgtaccgctc agagtacaca
    58861 tgaatgacca agctcacgga actgatgagc accatcatga ctgcagccaa ccgatcgacg
    58921 agaatggtat acgaccacgc tcctgaggga gcgccggcga gcgtgaggcg gatgggatcc
    58981 ccataggaga ccagcgccag cgtgaccagc gatgtcagcg cggaggccca taacgctcct
    59041 atccccagcc gtgacactcg ttcgcccatc tctcgccaga agagggcgat tgatagcgtg
    59101 ccgaagagtg gcgccagaat cgttaccaat ggaaagattg cttccatcat aggtggctct
    59161 ccaaatgaat catcatgctt cctctacagc aagaacaggg cctgattcag acgtgcatca
    59221 gactccgaat gaatccggaa aatgcgtgcc tgtagaggat gtccgctgca gccacgggtg
    59281 cctcaacgct taagtattgt gcgcggacgc acagctgtgc atatgcgcac aatcaggaag
    59341 ttaagtgcaa gtcaggctta cactgatgtg ggactcggca gcggggcatg ctgcgtaccg
    59401 acgcgccttt ccccctgcca ttggttcccc agctgcggcg aggctttaca ccccgtcaca
    59461 cgtccttcga ttcggaccgt ggcttgatgt gataaccggc ctatgatagc gtttgcaatg
    59521 catgctgtct gcaggacaaa ttcgcaccag tgaacgccgt cggattgcac acgtcttgcg
    59581 cggctggatg atcctgtggc tgctcgcctt gccgttgttc cacatccatc ccgaaacaga
    59641 cccacatcat ggggaggccg gtcatgtcca tgccgccgca gtgcataccg tcttctccgg
    59701 tgacctcgaa ggcgaattcg gcgaacatca agagacggcc tctgtaagac ctcatccagg
    59761 tgaagggccg gcggtttcag ccgaaggtgc gcatgcctgg agttccgatc cggaactcaa
    59821 cctgtctcta ctcaatgacg ccaccgaccg taagctggcc aaaccgctac tgacgcatct
    59881 cctgtttgtc gcccatcatc tggtacccct ctttgaccag caggacggtc cgcacggtga
    59941 gccattgtcc tccaccgtca ccatgctctt caccagggat gtgccggccc gtgctccgcc
    60001 ctccccgttc ttcagctaag tatcacccgt tttcaccgca gacattctga tcgcacgcac
    60061 gtctccgcga ggcctgttcc ggctcccgcc cgacgatccg tgcggaatgg aggaggtttc
    60121 cgatgatgcc tcgtatggtc ctttccctgc tgatggcctg cgtcttcagc caatcgggcg
    60181 ccttgtcctg ggctggagaa gaacggcctc gcgcctattc gctgtcggac gttctgacgt
    60241 tcgcggcgca acacaatccc gtgctcgcgg gagctgaggg actggtgaaa caaagccagg
    60301 ggcaacagat tgctgccggc gcctatccga atccttccat caccgggatt gccgggcgcg
    60361 gcgcgatccg tgaccccagt accggaaccc acgtcaccga gcggacgttg accgtggaac
    60421 aaccccttga atggaccggc aaacggcagg cccgccagga agcggcggat gccggcctcg
    60481 ccggcgcgaa cgccgctctg gaggacgccc ggctttcatt gctcgctgat gtcaagattg
    60541 cgttctatca cctcctgttc ggccaacgcg atgtcgaact gtccgctcaa aatgtcacga
    60601 atgtggaaga ggtcttgcga acggtgcagg cacgcgtgac ggccggtgaa gcgacatctt
    60661 tcgacagtct gaaagccgga gtcgaggtgc aaaaggcgaa gaaggaagtg gctcgcgcca
    60721 acaacgcgct gctggtggcc agggcacggc tgaataccct cactgccggg acactcggca
    60781 aggagttttc cgtgcaaggc gatttcgacc ggccgaagca gagcctcaat gcagatggtc
    60841 tgaccgcgca ggccttggag caacaccccg ccgttcgacg cttgagcaaa ctggcggagc
    60901 aggccgacca tacgctgcgt tttgagcgag aggcccgcgt tcccaacgtc agtgtgatcg
    60961 gaagttacca tcgcgaggcg ggcgacgaat ctctcacggc cggcctggcc gtgccgctcc
    61021 cgctgtggta ccggcggcaa ggcgagattc agtccgcatt gggcgccaaa caccgtgccg
    61081 atgcggagcg cctgcgggct cagcaggaat tggagcaggc catcacccaa catgcccagg
    61141 aagtgcggac ggcccaggag caactgcagg tcttcgaaac ggggttgctg aaacaggcgg
    61201 agcagaccct caccgtggca cgaacgagtt tccggcacgg cgcagccagt cttctggatg
    61261 tcctcgatgc gcaacgggtg taccgccaga ctcaactcga atatgcccag gtgcgagcgg
    61321 atctctccat cgcactggcc cggctcgaac gcgcgctggg aacatcatta tgaacgccga
    61381 ccgcagtgga tggactattg gagtgccgcg atgacgagac gactgatgac gatcggcatg
    61441 gtgcccgcgc tgctctggct tctgtctggt tgcagcgacc agggaacgcc gccaccaaaa
    61501 cctgccacga ccggcgaagc ccagacaatt accggtagca gtaaagccat ccacccgccg
    61561 ccggcagtcc aggctcgcct tcgaagcgaa cctgccgccc tgcgtgcggt acccacactg
    61621 atcacagctc ccggtgaggt ggccctcgac ctgaagcagg tcgccaaaat cacgtcccgc
    61681 atcggcggac aggttgagcg tatccatgtg caattgggag atcgagtcaa gaaaggccag
    61741 gcgctggtcg ccatcggcag cctgcaactc gatcagttga tcgaggaata tctcgtcgga
    61801 aaggcccaag ccgacgtggc ggaaaacagt tttcgccgga ccgagaaact gcgcgccgac
    61861 gacatcgtca cggagcgcaa gctgatcgaa gacaaggggc tctatctcga gacgcaggcg
    61921 cgttatcaac acatccgcga acgactgtcg aacatgggca tctcgacgga tgacttgaaa
    61981 caaggccccc atgagaagag ccatctttac accctcaccg ctccgatcgc agggaccgtc
    62041 gtcatccaga acgcggtgcg cggacaggga gtcggccccg gtgatgaatt gtttgagatc
    62101 gtcgatacca gccgtgtctg ggtctttgcc aatcttccca tcgagcaggc caggcaattc
    62161 aaggagggcg actccggtac catcacgccg aaaggcggcg aagccatcgt cgcgccgctc
    62221 acctacctgt cgccggtggc ggacgagacc acccgcacca ttcgcgtgcg gttcgaggta
    62281 gccaatctcc aaggacagtt gaaaccacgc gagtatgtcg aagtggcact cggctggtcc
    62341 ggggccccag tgatcaccgt tcccatgacc gccatcacca cgatcgacaa aacacgcggc
    62401 ctgtttctcg acaccgcgga tggctacacg ttcgtgccgg tcgacgcagg ccgcgaaggc
    62461 ggcgggtggg tggaaatccg tcggggtgtg aaggaaggcg accggatcgt gaccgacggc
    62521 gtgttcgatc tgaaaaatgt gctgctgaag gaacacatcg gctcgggcga atgaggcgcg
    62581 catgcaacag ctactgacgt tttcactccg ctatcgattc tttacgctcg tggccatcgt
    62641 cgtcgtgatt gcctccggcc tgtggtcgtt cgcacacctg acgatcgatg ccgtccccga
    62701 cctgacccca gtgcaggtgc aaattctcac acgcgccccg gcgctggggc cggtcgaggt
    62761 ggaacaattc gtgacgtttc ccatcgaagc ctccttgaac gggttgccgg ctctgcgcga
    62821 gctgcgatcc atttcgcggt acggcctttc cgcagttacc gcgatcttcg atgatcgcac
    62881 cgacatctat cgagccaggc agttcgttgc cgaacgattg gctcaggcca tggaacgcat
    62941 cccggcggaa tacggccgcc cggtgatggg ccccctcacg accggactgg gcgaggtcta
    63001 tcaattcacg gtgaaaggcg aaggctttag tccgatggcc ctgagaacgc tgcttcagtg
    63061 ggacatcggc atgaagttgc gcgccgtgcc cggcgtagtc gaggtgaata tatggggagg
    63121 cgatccgcag cagttccacg tcatcgtcga tccctcgaaa ttgttgtcat tcaaattgtc
    63181 catgaagcag gtgttcgatg cgttgcagcg caacaacgcc atcgccggcg gcgggtatat
    63241 cgaacaccag cgcgaacaac tgtcgatccg cggcgaagca ctggcaacac agctcagcga
    63301 tctggcccag atcgtggtag cccacgggcc tggcggcgtc cccatttata ttgctgacct
    63361 ggccgaagtg aaagaaggat cagcgctcag gatcggagcc gccacggcca tgggtgaggg
    63421 cgagaccgtc atcggcatgg tgcagatgct ggccggagaa aatgcgcagg aggtggtcac
    63481 tcgcgtgaag gcgcgggtgc aggacattca ggccaccctc cccgccggcg tcaccatcga
    63541 accctattat gatcgcacga tcttcgtctc gaaggtcatg cagaccgtcc gcaacaacct
    63601 cctggagggt ggtctgctcg tcatcgctgt cttgtttctc tttctcggcg acctccgcgc
    63661 agggctcatc gtcgcctccg cgattcccct cgccatgctg atcgccttca tcggcatgat
    63721 gcaggccggg ctctccggca atctcatgag cctgggtgcg atcgacttcg gccttctagt
    63781 cgatggctcc gtcgtgatga tcgacaacat cctgcgacga ctgggcgaga agccgccacc
    63841 aaccagggaa gcccgcttga aggaagtgct ggccgccggg caggaggtgt tgcgccccat
    63901 gacgctggcc gtgacgatca tcattctggt gtacgtcccg atcctcgcgc tgaccggcat
    63961 cgaaggcaag atgttccgcc cgatggcggt gaccgtcatc atggccttga ccggttccct
    64021 cttcctcgcc ctgacggtca ccccgttgct gtccttttgg tttttacgcg ccactcccgg
    64081 cagcgaaacg acggcggtca tacggggcgt gagaagagtc tatgccccat ggttgcagcg
    64141 ggccgtggcc cgcccaatct ggcccgtgtc gattgcggtg gggctttttg ccgtcagcct
    64201 cgtggccgga agcaggttag gaatcgaatt tgtcccgcgt ttggaagagg gggacctcgc
    64261 ggtgcaggta tggcggctcc cgagcatctc gttgagcgaa tcggtcacca ccgcgttgga
    64321 tatcgaacgg gtcctgaggc gttttcctga agtcacgcgg gtcgtcacgc gcaccggcag
    64381 ccctgaagtg gccacggacg tcatgggcgt tgagatgtcc gatgtcttcg tcatcctgaa
    64441 accccagcgc gattgggtca cggcacacag ccgcaacgaa ttgatcgagg cgatgaaacg
    64501 acagatcacg gaccaggtac cgggggtcgg acttggattc acccaaccga ttgaaatgcg
    64561 cttcaatgaa ttgattgccg gcgtgcgttc ggacctggcg gtcaaaatct tcggtcccga
    64621 tctcgacatc ttgaagcagc aggctgaacg ggcggcgcgg gcgcttcaac atgtaccggg
    64681 agcggctgat gtcaaagtgg agcaggtcgc cggcttaccc ttactgcgcg tcgttgtcga
    64741 tcgcgctgag atcgcccgtt acgggctcac ggctgaagag gtgctcacgc ttgttcaatc
    64801 ggcccgggtc ggaacgattg tcgggacggt cgtgcagggt tcccggaggt tcgaattagt
    64861 ggtgcgattg gcggaccggg tctcgcgtga tcctggttca ttgggcagtc tgctcatacc
    64921 gaccatgcat ggagaactgg ttccgctctc acgcgtgacc agcatcctcg tcgattcagg
    64981 tccggcacag gtgagccggg agcatgtaca gcggcggatt gtggtcgaga ccaacattcg
    65041 cggacgcgat ctcggcggat ttgtatcgga ggctcaacag gccgtggccc aggcagtcac
    65101 gctgccacca gggtatgagt tgatctgggg aggacaatac gaacacttgc aggaggcggg
    65161 caagcgtctg gccatggtcg tgccggtcac actgatgctg attctcggca tgctatctgt
    65221 catcttcggc acgctacgcc cagccctcct cattttcctg aatgtcccac tcgccttgtc
    65281 aggcggcatc ctggcgctgt ggctgcgggg attgccgctc agcatctcag ccattatcgg
    65341 gttcatcgct ctgttcggca ttgccgtcct caatgcggtc gtcctggtca gccacatccg
    65401 ccagctggag gcggaagggg tttccactcg ggaagcggtc ctgcacggag cgatggatcg
    65461 tttgcggccc gtgttgatga cggccctggt ggcaagcctg ggctttctgc ccatggcctt
    65521 ggccaccagt atgggagccg aagtccaacg gccgctagcc accgtggtga tcgggggatt
    65581 gctgacatcg acagccctga ctctgctggt gatcccggcg ctgtactcgc gcctggtccc
    65641 gcaggctgcg tcccggcccc atccggcagc tgtacaggcg gacgatagcg ccattcaggt
    65701 gtaacccccc gtggcatcac gagaaatcga ccaccagccg cacgtgatac ggcgccgctt
    65761 ccgactcttt tctggacaga ctttcatccc ggcggtaagc tttcctctac gaggcgaagt
    65821 gtctgcacgc aagccagggg cctgcgcagc cgctcaagcg aattcataac cggcccattt
    65881 tcccgaggag tagtttatgg cgaccatcat ggtgatcgac gacgaacctt ccatccgcgg
    65941 cctcctgcgc gaggtcctgg aacgatcagg acacaaggtt gtcgaagcca aggatgggcg
    66001 tgaagcgttg gatctctacc agaagcataa ggccgatctg ctcatcatgg acttgttgat
    66061 gccggaagtg gatggattgg aagccacgtt gcagctcacc cgcgaatata tggacacgaa
    66121 aatcattgcc atgaccggcg ctcaggggga tcggaatttt ctcgatatcg cccggctctt
    66181 cggcgcgcac cggactttcg aaaagccctt cgacttgaag gaaatgctga acgccgtgga
    66241 agcggagctg gcgcagaaat aacctcgcac cggccaatcc gccggctcac ctgacgcact
    66301 cgtcactccc gggaaaaact actggaatca ccgccgctgc ttctgcaaga attcaccgtg
    66361 cagcagaccc ccacagccag gttctcgtgt ttctaacccc cactcgcgca tggcgtctct
    66421 ggtgtgcctg gagcatgctg attgtgctcg tgggcatcct gcctctccat aacttcgtcg
    66481 gccatgcgca ttggcagtat attcgttggg tgccgacgcc ggatcaattg agttccccgg
    66541 cgatcctgct ggatcttgcc ttcgatgcgg tcgccaatat cctgctcttc gttcccttcg
    66601 gcctgctcta cgcgcttcgc ggcctggaag gcaaacctct ttctccgcgc catctgttgt
    66661 tgatggcctt tgcgctgtcc ttcagcatcg agtactacca ggtctattgt cataaccgca
    66721 gcacgtcgct gctcgacctg ctggacaatg tcttgggcgc atacctgggg atgaaagcgg
    66781 gggaagtgta tatcagaaag aatgcgtcag cagtggtcga accggccgca taagattatt
    66841 gccagcctgt cgggtcatag ccccggtctc gtaagcaccg atcgacataa ttcgtaaacg
    66901 ccgcgccggg cggagaccgt ttgaacagtc ctcgcaaaaa ccctgcggta gcgccgctgg
    66961 ctgcgccgac cattgcaccc cgtcccgggt gtccgagaat ggctccgccc accgctccgg
    67021 ccgcagaacc gacagcgccg cctcccactg tccccgtcac gacctgggcc gtcttgcctt
    67081 cactggaaga cgccccggcc tccttcgcca gcgccatgca atcctggata tcagcctgcg
    67141 cggcatcggc cccgacttgc tggtagtgag cattgggata cagaatgggt ttgggggcag
    67201 cgcagccgga aagcacgaga cccagaacac agagacaagc catttgcctc atgacacaga
    67261 cctccggtgc gctgaatgtt tgaagggaac gtcaggccgg cccggtgatg gtacggtata
    67321 aattagatcc ggccacccaa gtccttgcgc tgcagcgtac gcatcatgac ggaatcgttg
    67381 tacgccacct cgctgagcgc atccatgatg tgcagccctt tggcagtgac caactctttg
    67441 gccttggcat aattcttcgc actgaaccgc gcgaccgcag gctgaccatt cacaatctgg
    67501 caggccagga cttcaccgcc ggcgacatcc atcgttcccc cttcttcaac cagctttttc
    67561 gcctgcggca tcgtaatctg atgcaattgg gcaaattcct ccagcccgcc ggtttccacg
    67621 agacgtccat cgggcatgat gcacaaccgt ttgaaatggg gagaccagtc tttcctgaaa
    67681 tcgatgaact tgatgtcgcc gcctttggcc acggcacgat ccagcgtgcg gtaatcgagg
    67741 ccaagatttt tctgggcgag tttggttttt aattcctccg gcagcgtccg gtaaaagatc
    67801 tctccggcgg catcgatcaa tgagacggcg cgcgccctgg cccattgctc cgcttcggcg
    67861 aactgcatga gatccagcac ccaggtctgc ttgggagaag cccccgtcgg atctacccgg
    67921 caggcaaaga gcccccgcgt gagcagcccc cccacttggg gatacacgta cacgcccccg
    67981 tctgtcagga gctgcagcat ctgctccaac ggcacatcgt aactttccgc caggatcttc
    68041 gcatgcgccg ccagatcttc cggcaaggtc atggcgcaca ccgtgacgtt tcccggcgca
    68101 tcgaattccg agtaatcctc gatcacgttg tacagaatcg gcggctttgg cttctcgatc
    68161 ttcttcttga tcttgaacat aatgaattcg ttcctcgtcg ttcgtctttc gttcacacta
    68221 cgtgcggtaa attactgcgg tgcaatcacc cgcgctggag gtggtagtaa gccggcaagg
    68281 tcagcagccg cccgtcgtcc accggtccac caatggtaaa gtgatagagt gattgcagcg
    68341 tcaggtcctt gatgccgacg atctcgtgca cgggatcatc aaaataacag ccgatgcccg
    68401 tccctcgcac gccggctgct tctgcctcga gatacaatac ctgcccgacc agccccgctt
    68461 cccagaaaag gcgtggatac caccaagccc cgccctgccg caagcgcccc tcaaattcgg
    68521 ccaacatgcc gagtgaaaac gcgctgtcac cggcgatgcc ttgatgacaa ctgacttggg
    68581 cagccaggcg ttgcgcattg ccttgcagca gccagtacag cggaagatct gtcggacacc
    68641 caggcaccgg ggtccactcc aattcaggat tcatcgcctg ctgcagaagc ggcagcttct
    68701 tcgcatcccg cgccatcaca tagaggcccg gcgtcaggcc ctccacccgg tgcacgaaca
    68761 acagcagatg aatcgccggg tcccagggca agagatccca cggcatgggc cgggccagtt
    68821 gcggttggct ggcgtgaggt atcagccgaa ggagaatctg aaaaaacgta gcgctggaaa
    68881 ttgccgtgcg gccatcgaac gacacggcgc tgcgacgctg tctgatgatg gtcgcggcgc
    68941 cgcgcgtggc ttgtgggtcg tgacgtggtt ccgaatgcac gggcggctca acggggagag
    69001 acaccaccag gcgctcttca cctgttttcc atgaggcctc cgctgcttcg tcgatcgctt
    69061 cccaatgcac gccatgctcg cgactcaata ggttcgcatg gccatgccat ggtcccggcg
    69121 ccagatcctt caccaccgtt tgatccacac ccaacggcag gttttgaaca tcgtgcttca
    69181 tgttgggtga aggccagatc acggcaagac agtcgggatg ctccggttcg gcctcaccaa
    69241 aatcctcctg gcgatgggtg cccaacagcc gcgcgacctg attttgttcc accccgtcca
    69301 gcagcaccat cctccaacca agcgtcgccg cggcgatacg cgcggacccc agggcgtggc
    69361 cgacgtcgtg gttgcaatag cgaaaggccc gttctccata cttccaggct tcgcgccaat
    69421 ggacggaact gaggccgaac aaaaatgcgc cgtccggaaa aggctcgagc agctgtgtga
    69481 tctgcgcagc gggaaagccc gcccgtaact ccacgctatg ttccttggct gcgtagtgat
    69541 agaggccggg cggcagatcc aagccatcga ccggcggaac gatcacatag ccttcggtcg
    69601 gatgcagatt gccggacgaa ggattgctgc gaagggccca ctccgactcc cctgctcgct
    69661 tccaggccga cagcccgagc gccagttcaa aaaaccgtga gatcgtcttg atggtcaatg
    69721 gccgggaggc cactgcgcct ggttgataga gagcatcgta agagggcgac tgcggatcgt
    69781 catccgggct cagcaacggt aaacggatca gttgcgcacc ctcaaaccgc cgaaacggat
    69841 tgggttgatt tgcccaatcc agaaatccca acgatcgcgc gtatcgattg aaatgatgct
    69901 tggttcgaat gtgataggcg atcacacggt cgattggatc tgtcgaagta gtcccggcag
    69961 acgcgccgga agacggttgg tcgagagtca tgcgcgcagt ctaacgaggc ggaggaagga
    70021 aagtaaacgc acagtccgtt cagcccagga gtcttgcgat cccgaaggcc gccgctgccg
    70081 cgagtcctcc gacgaacacc gtttggactc cgccacgcca gggcggcaca ccggtcaatc
    70141 cacttttcac gtatccgaaa acaaacaagg ccacgggcgt cacggcaatc gaccaccaca
    70201 gcgccgtcgc aatctcgtgg aacaccatgt agggcaacaa cggaatcaat cccccgatca
    70261 catacgagag cgcaatcgtc caggcgctcg acctggcacg cgccggatcc ggttcttcca
    70321 atcccaactc aaaccgcatc atgaagtcga cccaccggcg ggggttggct cgaagggagg
    70381 cgatcagcgg agcaatgtgt tcatcgcgca acccgtagcc acgaaaaatc tcggccacct
    70441 cctcggcctc cgtttcggga atatgctcag tctctcgcac ttctcgcttg cgttcagcag
    70501 cgtaatgctc aagatcggtc ttggccgcga gatatccgcc cagccccatc gcgatcgagc
    70561 cggcggcgat ttccgccaat cccgccgtaa ctactaaagc ggacgaagcc accgcacccg
    70621 acaatccggc ggccagggca aaggggaccg tcaacccgtc ggccatgccg atgacgatat
    70681 cgcgcaccgt cgccgtggcc gtgaaatgtt tttcactatg tggagtcgcc ggcataggga
    70741 tactccttcc aaacgtgaac agcccgatga gctatcggca tcgaatcttg ctggctgcgg
    70801 tggtgcttgt gtacgcgggt tgacagtgct ggtcaaggga aggggtcgga gtggtggagc
    70861 ggctcctccg gggtcacacg gatgtcctga tggcgtgggg agcaaaaacc gagttgagtg
    70921 aagcggcgtg aatgtcgccg gcgacgcctc ctaaagctgg catcgccggc agatggttca
    70981 gtgatgagcg taggactagg ccgccattgc agcagggctg aacaacttca ccgtatcctg
    71041 caattggtcc agcgcctcct gcagcaacat cggccgatct tcggcgttga aacgggtcgt
    71101 ctgcaaggcc gtcgcctgaa agggcaaatc tttgggcgca cgcgtaggag acagctcata
    71161 gtggtcggcc accacgcctg ccagatgcac catcgaagca tggagtgaaa aatcgccggc
    71221 atcctcgggg cacaactgat ggcggatcgc ctgctcgatt tgaacgggca tgccccaaga
    71281 atggaccagt tctgcgccga cttccgtgaa atcacatccg aaattcgatt gctcgacttc
    71341 cgccagcggt tcgcccaaat taccggcttc gatcaatgcc gattgcgccc gttcgggaat
    71401 tgtctggtac agcaccaggt gcccgatgtc gcgaagcaga ccttcgatga aaaaccgctc
    71461 gctgtcttcg atcccgcagg ctttggcaat cttgcccgcc atcaatgcgc agagcacgct
    71521 tttccgccaa taagcggaga gatccatgat ctggacggtc atgccactga agctgcggcc
    71581 gaccgtcgtc gcggtgacaa gatcgcgaac cgcctgcatg ccaagcaaat tgacggcccg
    71641 ggttaccgta tcaacttgtt tcggaaagcc gtagagagga ctgttgacga tcttcagcac
    71701 gcgggcggaa atggccgggt ccatcttgag gacctgcgcc agatcgtcca tagtggaatc
    71761 agggttgtcg acgacgtcgc ggacacggaa atatatctcc ggcagggtaa agacatttga
    71821 gcaggactga a
//
LOCUS       DAOQDS010000011        71065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_119_length_71065_cov_19.323884, whole genome shotgun sequence.
ACCESSION   DAOQDS010000011 DAOQDS010000000
VERSION     DAOQDS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 71065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 71065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71065
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119_length_71065_cov_19.323884"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1529
                     /locus_tag="PK589_04035"
     CDS             216..1529
                     /locus_tag="PK589_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNJ18795.1"
                     /translation="MDIIVLIFLILLSAVISVVEVGFYSVNDTKLRALADTGSKRAEM
                     ALHLRTDPQRLLLTILVGDRLVDTATASLATIIALNRFGGQGLEGVLGEAFAVLVGIL
                     TFVLLVFADLVPKTLAAKYSVPVVLNMAYPAYAAQQVLTPIMFFVVPLIYKLTGGKGL
                     NVPFVTEEELKIMLDQSSKSGAIEAQEVKMIKNVFQLKDITAEDCMTPRIYMFSLDCN
                     QYLREAKELLFKSKYSRIPLYEGTLDNIIGILYKTKALTALAQGHTEMKLRDIAQPAL
                     FIPHTKSADDLMKQFQLDKRHMAIVVNEFGGVMGLVTLEDLLEEVVGEIVDETDITEE
                     LIKRIGKNQILVHGRTEVRKVNDFLKVDLGDDAVTISGLVQHELGRIPKVGEEVHIAN
                     CRLVIHEADPRVIKSVQIYKEDKHPALPDQPVEEHVPVTQASPDR"
     gene            complement(1504..3531)
                     /gene="ligA"
                     /locus_tag="PK589_04040"
     CDS             complement(1504..3531)
                     /gene="ligA"
                     /locus_tag="PK589_04040"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNJ18796.1"
                     /translation="MSNPPMADRSIEQRITALRREIRHHDHLYYTKDRPEISDAEYDR
                     LFRELVDLETAHPDLITADSPTQRVGAPPLSELTKVSHEKPMLSLDSIVDQEDVRAFD
                     QRMKRELETDQPVYTAEPKFDGLSVELVYDQGRFVRGATRGDGMIGEDVTVNLRTIRS
                     LPLQLHAETGPPAHLVVRGEVYMRLDEFQSLNRRMTERGEEGFANPRNAAAGSLRQLD
                     SRITASRPLTVTCYDIMAIAGPMPATHWDELEALAAWGLPVPEHRQRCRSIDEVLTFH
                     QTTDAMRDDLPFEIDGVVVKLDRRDWQDQLGSKSRSPRWAIAYKFAPRKEITVIQDIA
                     VSVGRTGTLTPLALLKPVEVGGVTISRATLHNADEVARKDVRVGDTVKVERAGDVIPA
                     IAERVPVPGEERQAPFVMPDHCPVCGSAVAREGAYFYCTGQTVCMAQLKGAIEHFASK
                     SALNIDGLGKKTVAQLVDAGMVKDLADLYSLTKEQLLTLEGFADRSASLLMDSIERSK
                     AVSLERLLMGLGIRQVGQHIARVLAKHFGTLPRVMEATQDELLHVREIGPEISESLAS
                     FFTEARNREVIERLVARGLTVAPPVAEASPGGRTLTGKTFVFTGGLAAYSRDQAKELV
                     ERQGGQVSSSVSKKTAYVVAGIDPGSKLDQAQKLGVKILTEAEFTDLVTPG"
     gene            complement(3524..5422)
                     /locus_tag="PK589_04045"
     CDS             complement(3524..5422)
                     /locus_tag="PK589_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18797.1"
                     /translation="MAAPSSTADDVFRLSPRIELFPVLHGSGDVAQEVRDRLTDRRYD
                     CLAVPLPPSFEHPLEEAVVDLPTISVIVQQEPDSETGATVNYVPVDPCQAVVMGIRVA
                     IGEGMYRAYIDRETSVVEPIPFVSPDAYALKRLPYAAFAASMVPTLTTPAEGSQQQAR
                     IAWMAFQLHQLELEYERILCLCHFADWPWIRQAYQQRADYHEPDADIRRPEQYLVEPA
                     SLYFVLGELPFLTELYERRRATVHSDRHLSIDGIKELLIETRARWQTAHDEEGHTVPN
                     WVTPQLLQLYLQYVRNHALLDRRLTPDLYTLVLAAKQMAGDDFAIRLLETAKSYAYQE
                     IEGKHLPQLSAGLGQFALPDEQIVRATNRLQGAPVTWRSLSLRPRPPRRTSQRWSYLW
                     NPNRQCSWPPEDSKIESFHTHVRDQAKAIIGADLAKSEKFTTSMKDGIDIRESLRHWH
                     QRAKPGRPNRLELYVKEIPPARGNVDTVIFLFDTPADPDQYSWQATWYAEHAQESTLC
                     FYATPFLNNMIAPGIGQSLYGGALFIFPPRPIPDIWTDEALGFATTLEERLIAAGAAH
                     SHETHVALVTPVPPKARWRQIASRSGRRLVPIPLSRFSGQLIDRLRRFHVLNGQDIRS
                     FAAQFIRE"
     gene            complement(5422..6375)
                     /locus_tag="PK589_04050"
     CDS             complement(5422..6375)
                     /locus_tag="PK589_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ18798.1"
                     /translation="MASGAQPASSRVISIEGITLHLAQPMSMGQEWIGSREILKQLLA
                     CWLVIDERDLPLSPRITGQPGIGKTTLAMAGARERKQDLYVFQCTADTRPEDLLITPV
                     LAESGTISYHASPLVTAVLTGSICVLDEGNRMNEKSWASLAPLLDHRRCVESIIAGIL
                     IKAHADFRCCVTMNEDASTYEVPDYILSRLQPTLGMGFPAREDELAILRYHLPFAPAE
                     MLALTVEFLQEAHQLSLEYSVRDGIHLLQYALKRRAQDPTDPLSVDAAWRESLIKVLG
                     EEALDLPMQSRRRKRALGDQALPQGLGDFFFGSDDPLHPGR"
     gene            6611..7297
                     /locus_tag="PK589_04055"
     CDS             6611..7297
                     /locus_tag="PK589_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNJ18799.1"
                     /translation="MMSEQQGRLRRRSVVPIGLLILGSLGLGGCVMSEKYEAEKARSL
                     NFQRLLAQEEKRSAELDAEVKRGKRELSEYEARNRELGAQVEAVRQQAAQIQEEAQAM
                     KEASLLEKRAHEDMKRLTTIPKAKKAAPKKDFAAAVDEALKTEVPSDLSLEPATGAAK
                     DALTHSDLMDGAGATAAAATGTTHTVRPGETLYRIGQKYHVAPDQLRKWNNLKDNTVG
                     VGQKLIVSQP"
     gene            7319..8815
                     /gene="trpE"
                     /locus_tag="PK589_04060"
     CDS             7319..8815
                     /gene="trpE"
                     /locus_tag="PK589_04060"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNJ18800.1"
                     /translation="MTKQQYSLTLDEFRGLATEGNLIPLYREILADYETPVSAFAKID
                     QGPTAYLLESVAGGENWARYSFLGSGSSAVIHEEKGDLILTRGKKRLKIQSRGNPLER
                     VRELMEEYRPVTVPGLPRFVGGAVGYLSYDVVRTFEELPSLRKDSLGMPEVAFLLTDT
                     LLIFDNVSQKIKVVANAYLESTTDRAVREAYRHATARIDKMIARLKRPVRQPRVKRRR
                     KPITFTPNMNRADFEKMVTRTKEYIRAGDIVQAVLSQRWETQIHTTPFQLYRALRVVN
                     PSPYMYYLRVAGVELVGSSPETLVRCEDGQISLRPIAGTRRRGNSPDEDQDLARSLLA
                     DEKERAEHVMLVDLGRNDVGRVAARGSVKVDSLMQVERYSHVMHIVSQVTGQLEKGKS
                     VYDVTRACFPAGTVSGAPKIRAMQIIEELEPTRRGPYAGAVGYFGFSGNMDMCINIRT
                     VVIKGRQAYIQAGAGIVADSVPEHEYEETCNKARAMMKAIELAEQGLE"
     gene            8863..9426
                     /gene="pabA"
                     /locus_tag="PK589_04065"
     CDS             8863..9426
                     /gene="pabA"
                     /locus_tag="PK589_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNJ18801.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEDVVVYRNDKISIPEMESLKP
                     SRLVISPGPCTPSEAGVSVEAIKHFGGKLPLLGVCLGHQSLAVAFGGEVIRAERLMHG
                     KTSMVRHDGRTLFRGLPNPFEATRYHSLIVNRKNLPDCFEISAETAEGEIMGMRHKTL
                     GIEGVQFHPESILTTAGKELLRNFLKL"
     gene            9466..10482
                     /gene="trpD"
                     /locus_tag="PK589_04070"
     CDS             9466..10482
                     /gene="trpD"
                     /locus_tag="PK589_04070"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNJ18802.1"
                     /translation="MIKDAINKLAERADLTEQEAETVMGEIMDGSATCAQIAAYLMGL
                     RMKGETVEEIAGSVLAMRARATHIRVKDSLVLDTCGTGGDRAHTFNISTTAAFVVAGA
                     GITVAKHGNRSVSSKSGSADVLAALGVAIQLPPEQVADCVNEVGIGFLFAPLYHSAMK
                     HCAQPRQELGIRTLLNILGPLTNPAGARLQVVGVFDAGLTELLAKVLVHLGAQHCFVV
                     HGMDGLDEITVTDRTRISEGKAGVVSSYTIDPAEFGLARVRPKDLVGGNAEDNAAITR
                     DIFRGRKGPKRDIVCLNAASALVAGRKAKTLQEGFELAQRTIDAGAAMEKLEQLIAFT
                     KKAS"
     gene            10487..11311
                     /gene="trpC"
                     /locus_tag="PK589_04075"
     CDS             10487..11311
                     /gene="trpC"
                     /locus_tag="PK589_04075"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNJ18803.1"
                     /translation="MILDRILEHKKAELRHKSSRGYLADLKTRIRDAGPTLGFAVTLD
                     ARRTADRPALIAEVKKASPSLGLLRPEFEQRFEPVKIAEAYREHGASAVSVLTDKDFF
                     QGDLAYLADVKRTVGLPALNKEFMVADIQFYEARAYGADAVLLIVAGLEKRQLIDFSA
                     LAKELSLDVLVETHHERELDTVLEWLPDVRLIGINNRDLKTFSTDLGVTLRLAKRIPA
                     DKMIVSESGIHTRKDVERLVEAGVHAMLIGESLIRAQDTGAKIRELLGDPPTKEQS"
     gene            11308..11622
                     /locus_tag="PK589_04080"
     CDS             11308..11622
                     /locus_tag="PK589_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18804.1"
                     /translation="MRTLLLFGLMAMGLTVAGCVNQEIHEYSPKWDSWMGSTKDDRIK
                     EMGIPTRCHSFKDGGEVCEWMIPQQDGRQDLIGLTFNPKGQACQWSYRGFYGMQKSQT
                     SC"
     gene            11626..12258
                     /locus_tag="PK589_04085"
     CDS             11626..12258
                     /locus_tag="PK589_04085"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNJ18805.1"
                     /translation="MTVKVKICGLTNAEDAAVAVEAGADAVGFVFHKKSPRCAETEAV
                     KAIVKELPPFVLPIGVFVNEEAKVVRDIMDSCGLALAQLHGDETAAYCESLGRPVLKA
                     IRLRDRRSFLALAEFQGRAGVRGFLVDAFSPDAYGGTGQVADWSLAAEAASVARILLA
                     GGLTPDNVIQAIEQVHPYGVDVSSGVEAKPGKKDHAKVRAFLEAVKRLPR"
     gene            12347..13537
                     /gene="trpB"
                     /locus_tag="PK589_04090"
     CDS             12347..13537
                     /gene="trpB"
                     /locus_tag="PK589_04090"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNJ18806.1"
                     /translation="MAIPDRHGRFGPYGGRYVPETLMPALLELEAAYQRTRKDKSFQA
                     ELKHYLTEYVGRPTPLYFAQRLTKRLGGAKIYLKREDLCHTGAHKINNAIGQALLARR
                     MKKPRLIAETGAGQHGVATATVAAMFGLECEIYMGTEDMQRQALNVFRMRLLGSKVTG
                     VDAGSRTLKDAISEAMRDWTTNVRTTHYVLGSVLGAHPYPMMIRDFQAVIGREARKQI
                     LSAEGRLPDCLIACVGGGSNAMGLFHAFVPDKKVRMVGVEAAGLGVESGKHAARFAGG
                     RPGVLQGTMTYLLQDENGQVNLTHSVSAGLDYAAVGPEHSYHKETNRAEYTSATDDEA
                     LAAFDLLAREEGIMPALESAHAIAEVVKCAPRMKKNQIIVVNLSGRGDKDVQQVARIK
                     GVTL"
     gene            13702..14502
                     /gene="trpA"
                     /locus_tag="PK589_04095"
     CDS             13702..14502
                     /gene="trpA"
                     /locus_tag="PK589_04095"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNJ18807.1"
                     /translation="MNRLDQTFGRLKAKGEKALITYIMAGDPSLQDTEQLVLELERAG
                     ADIIELGVPFSDPIADGPVIQQAAERGLQSGTSLRRILESVTRLRDMTQIPIVLMAYY
                     NNIHAFGEADFCSKAAAAGVDGLIVPDMPPDEAGPLREPAAAADLHLIFLLAPTSTAS
                     RRAYVAKESGGFVYYVSITGITGAKLNDMAGVRDNVAKIRKHTKTPVAVGFGVATPED
                     AARVAETADGVIVGSAIVRRVGEHGQDGRLVHEVGSFVRSLKAAMQPG"
     gene            14703..15758
                     /locus_tag="PK589_04100"
     CDS             14703..15758
                     /locus_tag="PK589_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ18808.1"
                     /translation="MAVARRKISRTTRKTTSTNSSVAARRKTTVRKPAPIGEDRLLRT
                     LQQGLFNTLSSAEGEGRSPIGVAAVFVQLFLQLTKVQAVALYVRDEQTREMACLAEAG
                     SSDPSVTELWLEGLKKAEQNAQIVSGELHGVRLHRINRLDGVVMFRAGKSQRLPSRKL
                     LSLLTAVEPWLAVLLDHARLTVKYAAKILRIQHMEQVSDILNSSLAEEEKLRRALDAA
                     VRLVEAEAGALFLASPDGTLSLSALGGERAAGLTALHSTVAAGVHRTGQAVLITQGRQ
                     DPRLVAGQGWQAALSVASLVSVPVRMGARAVGVLEVVNRRSGKPFSNWDVLELASLSN
                     QFGLAIDNLRRGSGVRA"
     gene            complement(15774..16379)
                     /locus_tag="PK589_04105"
     CDS             complement(15774..16379)
                     /locus_tag="PK589_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18809.1"
                     /translation="MTRHTFSTISVVLCLVMTGLLAGCGGLQEMWEGPAARGFYPKTM
                     AILPISGTYDSAREDVEEVVAKVLIKSRRVEKVVPPDQVTDTFQTTKDAFDALVTYFS
                     RMETTGQSDKNSAIKIGHALGADSLLVVKVNAWEYTREEGDNIARVGLSMRLVDAVNG
                     AIVWKARHQVQESYLFIRPDMKDLATKLAADMIKYMPPEKR"
     gene            complement(16426..17571)
                     /gene="traF"
                     /locus_tag="PK589_04110"
     CDS             complement(16426..17571)
                     /gene="traF"
                     /locus_tag="PK589_04110"
                     /inference="COORDINATES: protein motif:HMM:NF025106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HNJ18810.1"
                     /translation="MNRQVMSYVQYTAVLLAVLLPLQAAAVEFVFVGSRQMGMGGAGV
                     ATTTDSLATYWNPAGMAMSKKFDMRIQGGGQVVDRGDVFDTIKDINHINLNDTSAPNI
                     ARLQQQIDRMNKPGTNLTAAASGGFYVKGNFGEHALGFNVSDVATSGGFLRGPVGFTN
                     NGTNLTVNGQFAMNGLEVRQAALSYAYAFMDRMFSIGVTGKVIQGAAYTGATNIRGAS
                     DDVKVFEDIGRAKISSALGIDVGAMFRPSSWLRMGIVAKDINAPTFDAPNGDKFKLLP
                     QVRTGIAVNPYNSLTLTADVDITKNNTLTPGVYSQVLSLGAEQTILSELLSFRLGAFK
                     NMQDAKTPFIPTAGFGIRILALRIDIAGGYDFRDQAALASGSIAVTF"
     gene            complement(17679..18347)
                     /locus_tag="PK589_04115"
     CDS             complement(17679..18347)
                     /locus_tag="PK589_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ18811.1"
                     /translation="MIDIVDLYKTFPMGGRELVVLNNINLRIQRGELIAIMGASGAGK
                     STLLQILGTLDRPTKGTVSFDGQNLFQLTEQQQAEFRNKRVGFVFQFHHLLPEFTALE
                     NACLPAMIQKREMADVVGEATKLLGEVGLADRLHHKPGELSGGEQQRVSVARALMQQP
                     DLVLADEPTGNLDSHTGDALFALMRQLNRSRGTTFVIVTHNEKLSAQADRIISMQDGT
                     IISS"
     gene            complement(18420..19697)
                     /locus_tag="PK589_04120"
     CDS             complement(18420..19697)
                     /locus_tag="PK589_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ18812.1"
                     /translation="MALPYEIFIGLRYLRAKRRNRTISFNTIVSIVGITLGVAALIGT
                     VGIMTGFKEDVQAKILGTTAHILVNDRMKESMVDYEEQVKKVAGVPEVLAATPFIFRQ
                     VLLTSPSGVQGIILRGIDPAREGTVTELAHNLVNGNLDDLSHSTKVVIPEKEREPNGP
                     DTAMRPGIILGKELSMRLGAFPGDTLNVVSPVGPVTGASMTPKIRQFVVVGIFQSGMY
                     EYDSSLAYIELGEAQKFFNMGATVTGIEVKVADVFRAADVARSIEQQLGFAFWARDWM
                     QMNRNLFSALKLEKTMMFLLLVLITIVASFNIVSTLTMIVTEKQREIAILKAMGATRK
                     GIMRIFMLNGLIIGCSGAAIGVPLGYAFLWLIQTFWTFDPTVYYISRIPVHVLGSDVL
                     LVAGSAILISFAATLYPSLQAAKLDPAAALRYE"
     gene            complement(19700..21181)
                     /gene="lysS"
                     /locus_tag="PK589_04125"
     CDS             complement(19700..21181)
                     /gene="lysS"
                     /locus_tag="PK589_04125"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNJ18813.1"
                     /translation="MDELNDQRQQRIKKLDQLRQLGVAPYGTRFEIKDRAGQLVQLHG
                     EKSKEVLEQEQVGCTIAGRIVGLRRFGKAAFAVLQDGSDRLQVYLKKDTLGDQAHQIS
                     EGLDLGDWIGVTGILFRTKTNEFTVEAKTLTFLSKALRPLPEKWHGLTDVETRYRQRY
                     VDLIANPQVHQIFALRSRIISGIRAFLIERGFLEVETPMMHPIPGGAAAKPFVTHHNA
                     LDAELYLRIAPELYLKRLIVGGFPRVFEINRNFRNEGISTIHNPEFTMLEFYVAYADY
                     QDLIILTEQLFSRLAQDILGTMTIDYQGAQINLGSPWRRWSYHQAILEVNSLPPSVLT
                     NRDEALAAAKRLGVEVSPKESLVNILNEIFEETVEPKLEQPTFITDYPIEISPLARRK
                     DSDPSLTDRFELYIAGREIANAFSELNDPLDQRERFEAQAAQHAAGDEEAHRVDEDFL
                     RALEYGMPPTAGQGIGIDRLVMLFTNQASIRDVVLFPQLRPEK"
     gene            complement(21256..22399)
                     /gene="prfB"
                     /locus_tag="PK589_04130"
     CDS             complement(join(21256..22329,22331..22399))
                     /gene="prfB"
                     /locus_tag="PK589_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155970262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNJ18814.1"
                     /translation="MLDDVRTRVRTLGEQLAQLRGHLDLARMTAELNDIDVKTSQPDF
                     WKDTQAAAKVNRRKAALDRELSRWRDTERRQSDIEALLELALESGDAALEQELTAELE
                     PFEKAVAQFRIELLLSGELDANNAIFAIHPGAGGTESQDWAQMLLRMYVRWAEQKGFK
                     IETLDLQAGEEAGIKSATLSVNGPYAYGYLKAEAGVHRLVRISPFDANKRRHTSFASV
                     FVYPELDDDVEVVIEDKDLRVDTFRAGGAGGQNVNKVETAIRITHIPTGIVVQCQNER
                     SQLQNRNGAMKILRARLFEVEQRKKEAEFNAIVGEKKDIAWGSQIRSYVFQPYQMVKD
                     HRTGHEVGQVSAVMDGDLDGFIEAYLQRKMTGGKTPQPVAMTDDDV"
     gene            complement(22418..24052)
                     /gene="lnt"
                     /locus_tag="PK589_04135"
     CDS             complement(22418..24052)
                     /gene="lnt"
                     /locus_tag="PK589_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNJ18815.1"
                     /translation="MTISRARLILLACASGFLYPLSFPAFDLGIVAWFALVPLHIAVE
                     YASPRRAFRVGWLAGTLAFTGAMFWVITAMNVYGKVPLPIAVLVMLLLTVYLGLYLAV
                     YAGALSWIRIAFPTLGFLPAPFLWVTLELIRTYFLSGLPWALFGYSQYQWLAIIQFAD
                     HFGVYGVSFLLVLVNAALAETLIWSFKAYRGFQIRSVPWPSLVAAASGFGVALFYGTS
                     VLSAAQHATPRMLSVGLVQPNVEQAQKWDVAFRQETMSRYDRLTTGLGENLDLIIWPE
                     AATPFVFEMEPQYRAIVNDMARRADAPLLFGSPALRRYPDGKPFLLNSAYLLATDGQI
                     LGRYDKQHLVPFGEYIPFHNSLLFFLDKLVEGIGDFEAGPGSTLLMFKPHEKTPPMTA
                     AASNADFHLKFGVVICYEVIFPNLVREFAANGADFMVTVTNDAWFGPSSAPYQHFGMV
                     VFRAVENHVAFARAANTGISGFIDPYGRVLQQSPIFTQEALRGTIPVSHQQTFYSQYG
                     DLFAYACAILIALLCLTGYFSPNAEPAGGEPAGRPA"
     gene            complement(24149..24547)
                     /locus_tag="PK589_04140"
     CDS             complement(24149..24547)
                     /locus_tag="PK589_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18816.1"
                     /translation="MAIITTILLFVMWALISPRCDADAQSLPFRESHGTTGTLSPLGD
                     GSAIYSDPHGNTNPTQLGSGVPSHSFSGPHGATPGTLTPFGTPTPPNLLTPAPLLPLQ
                     PKGMAIPQPQSPASPSPPGRFAPPGGRPGR"
     gene            25045..25992
                     /locus_tag="PK589_04145"
     CDS             25045..25992
                     /locus_tag="PK589_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18817.1"
                     /translation="MGRKYDLVDLMAAIGLVATLFGGYLLVTAADGFWQAPITPTMSS
                     VIASNEPSTGMQYLQPVLGQAIVQDLLLERDAGAALSTSAMELNRAVSESERISTTLL
                     SPLVLAELRAFGQDTDHRARMQYVMGKSVVNQTRRGVSSGMLSPDQYGGEFNTNLIRT
                     AELTGQRMHDQFEATRQPALGQSIIEAIQEEDRMTATAQERVGRAVVQVTQAQEHYAE
                     AKADRQIQLASATVAAVRTDALMDRLAILENMSADRTMIAHHRAASADVSRGSLILAC
                     VGLIALFVGGLAFSTRKGEVDSIPLWKLETLLQLHRSGR"
     gene            26047..26169
                     /locus_tag="PK589_04150"
     CDS             26047..26169
                     /locus_tag="PK589_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18818.1"
                     /translation="MSWENWAVAIGLGALLMMFFGLMEMFFAGPRHPLDPSHRK"
     gene            complement(26173..26514)
                     /locus_tag="PK589_04155"
     CDS             complement(26173..26514)
                     /locus_tag="PK589_04155"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ18819.1"
                     /translation="MNASYRQRGTRRIPVQYPIYYSNGAFQTIGVTEDFNIEGGRVRG
                     REAVTVGMELVVIVIQAIPETPMLIRRATVRWSKGHDFGIALSGVPPQSEAELKAMAK
                     VMLPGLWSCLN"
     gene            complement(26511..27020)
                     /locus_tag="PK589_04160"
     CDS             complement(26511..27020)
                     /locus_tag="PK589_04160"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNJ18820.1"
                     /translation="MVRSASCTVLCAESRELTGVVPYMVFAAPIILSYMLLLVGLAVA
                     APGVGWGGSLIEFVPEAWRDGAHVPAYGVFAWLVMWGLLRRGWPVTHATSIGILATGV
                     FGLWTEVAQGTAPGREVSLHDVWSDLLGSTIAAAFMLWREKAIRMSKGFMVGHKRSHH
                     HLMKGTRSR"
     gene            complement(27127..27639)
                     /locus_tag="PK589_04165"
     CDS             complement(27127..27639)
                     /locus_tag="PK589_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNJ18821.1"
                     /translation="MPMPDAMRTEEVQAACDALVARVRTAVLLTLKQGHPFGSHVPYL
                     MGDDWSRVYLHLSGLASHTQHMLKDPRISLFIAEPDGAGKNPSALQRLNLQGSGAILS
                     PSHQSYDKVKERYLGQFPQSRMLFGFGDFAMWELHMSDAHLVLGFGHAYQAHSDNPRE
                     WQHQKPETKP"
     gene            27827..28228
                     /gene="bamE"
                     /locus_tag="PK589_04170"
     CDS             27827..28228
                     /gene="bamE"
                     /locus_tag="PK589_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNJ18822.1"
                     /translation="MRHMQWGLALAGICVVIGLTGCGGKQAEIKEDRLTVGRVQGEVK
                     VGMSAAQVAELLGSPNIVTTDDKRREVWIYDKVSTDRVDTSSSSYAGLIILGANSSDH
                     SSSQRQRTLTIIIKYDEEKKVRDFAYNSTQF"
     gene            28203..28820
                     /locus_tag="PK589_04175"
     CDS             28203..28820
                     /locus_tag="PK589_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18823.1"
                     /translation="MPITRLNSERPDTGSGHGWAAFILLLACGLFLGGCAEQTQPAQF
                     FELSPESSANRAMQTRLFETGADNELLSASAASLQDLGFQIEESVREVGLLRAAKERS
                     AREYGQYRNRFLIWLVSLGHLVIPVDLHQKIAATIVIRPANHSDARQEVRIIFYRVIW
                     KGDGQADRNYIPPGEQKMEMIRDPEIYQQFFAKLSKAVFLEPHSI"
     gene            28833..29270
                     /locus_tag="PK589_04180"
     CDS             28833..29270
                     /locus_tag="PK589_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18824.1"
                     /translation="MNRTQFIGAVGIVAAALALHGCVAPEPRQELFAPTDAQMKIRSA
                     QSRTFEVTDSAVAMRGVIAALQDLGFIIERANAPLGLVTAARFAEPNYYDVLGVTVTV
                     RPQEAGKTMIRANAIYNSKPIEDPKVYQNFFAAVERSLFLVKD"
     gene            29274..29876
                     /locus_tag="PK589_04185"
     CDS             29274..29876
                     /locus_tag="PK589_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18825.1"
                     /translation="MTGPRRSTLFSFRRSGGRPIVGSLALLAVLAGGCSPYEWRHENQ
                     WESRDQIWLSEASQVKVRAAQSRVFDGADRVKTIQAVVSTCQDLGFAVEVLDEALGIV
                     SAKKFDDYEPTRLRDPSYHLYRDDGLLILTPHFRSWGPFYHRSNLVRLTVTVRPRGEG
                     QLVVRASAQYYLKAIESPEPYQQFFRTLEQALFIQASTLP"
     gene            complement(29901..30611)
                     /locus_tag="PK589_04190"
     CDS             complement(29901..30611)
                     /locus_tag="PK589_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HNJ18826.1"
                     /translation="MPTEIEIQITPLFSTPLLVFTPADHTRINARLSTMILEREASVP
                     AHRDNEVVGWSSPHDLSMLEWAGEPLRDLFAPVLEVAKQVTTLSQRCGQGTGRPEWDV
                     VEVWANVQRNGGTNAAHSHPGSFWAGVYYVDVGEVSSNKDMGGELQIYDPRGCLPRML
                     APYLQYSMTELHDAGTSISYAPTSGQCVLFPGWLFHAVNTYHGKAPRISVAFNLDPVL
                     APGMMNGTQKRPITESRR"
     gene            30858..31061
                     /locus_tag="PK589_04195"
     CDS             30858..31061
                     /locus_tag="PK589_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNJ18827.1"
                     /translation="MSVARVTEIIAASPKSFDDAIHQGIARANKTLQHVKSAWIEDQK
                     VDIEDGKITQYRVALKVTFVLNE"
     gene            31071..31247
                     /locus_tag="PK589_04200"
     CDS             31071..31247
                     /locus_tag="PK589_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18828.1"
                     /translation="MKNVEMSVEGTWLTIKVDLSKEFGPSASGKTTIIASTEGNVTIT
                     GREEKIGLNVYRKK"
     gene            31436..32998
                     /locus_tag="PK589_04205"
     CDS             31436..32998
                     /locus_tag="PK589_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ18829.1"
                     /translation="MLRTQPSALKVPTPAILIVDDDPDIALVLKDLLEHSGYQVQVAV
                     TGAEALTRAKTDSFSAVLLDLMLPDMDGLSVLTLLKGIDPALPVIMLTAFVEVAKKHA
                     SLTEGAFGYLTKPYDGEELKALIKRAVGVKHLSLEAASAKQALTASEDRFREVVQTAP
                     DAIVLADGEGRILSWNSAAERLFGYRAEEVLDRSLTMIMPERYRDNHERALERVRTTG
                     DVRLKGTVVTMHGLHKDGREFPIEMSLSSWISSGTRVHCGIVRDITARKEAEARLLQQ
                     QIEQQALLDLIPAMVWYKDSHNRILRANRRAAESIGKTVADIEGRSTDEFYPEDAERY
                     HQDDLEVIVSGRPKLGIIEPHRAGGKKHWVQTDKVPYLDPAGKVIGVLVFAQDITDQK
                     RHEAALRENQDLVRIVMEATGDGIVIVDGDGNILAVNTRVAELFGYASGDLTGQSLQR
                     LIPSLFNFGTFELASDRGASTKGESLPLNPTCVGLHKDGAEIAVSISSTMLMTANGAH
                     GVVAVHPLGGPG"
     gene            32995..33468
                     /locus_tag="PK589_04210"
     CDS             32995..33468
                     /locus_tag="PK589_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18830.1"
                     /translation="MNLRFAFIILVLAFIALLLAIGVLTAIRWLRIHYPERANAILAA
                     SAVIVAGAVIMTIIEMNDRPMFRPHDLITLQEPVVARTILGDRGAGSATCVIDLHEHL
                     GVLEIEIERGALKARVESNNTSAPVFCPIGTEVRIDLNWLHRLTITRRQTQVSGP"
     gene            34003..35997
                     /locus_tag="PK589_04215"
     CDS             34003..35997
                     /locus_tag="PK589_04215"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HNJ18831.1"
                     /translation="MTAVEMGARQREISVSEFFTKNRHLLGFDNPRKALLTCVKEAVD
                     NSLDACEEAGILPDVTVKLEVVANGPAPVAPSQATRFRITVTDNGPGIVRQQIPRVFA
                     KLLYGSKFHRMRMSRGQQGIGISAAGMYGQLTTGKPVKVISRTGPRAAAHYFEVQIDT
                     KKNEPLVHENKQIEWSQPQGTEVVLEVEGRYQKGRASVDEWLEQTSIANPHVRLTYHT
                     PEGEQRSYPRTYHELPPSPKEIKPHPYGIEFGALLKMLQDTKSHTVASFLASDFCRVS
                     PALAEEICKAAKLSPTTKPRDVKPQAAETLYKTIQTTKIMAPPTNCISPIGEKAILSG
                     LYKQIKGEFYTAVSRPPAVYRGYPFVIEAGLAFGKGPEEPSKPQHAAVPLAEGEDQGN
                     DTEQARVIRYANRVPLLYQQSACATFKAVLSTTWRNYGVSQSRGALPAGPMVIFVHMA
                     SVWVPFTSESKEAIADYDEIQKEITLALREAGRRLGIFLRRRERAASEFRRRNIFELY
                     IEEVVAACNRLKGGNLPTEKLKEQLHKIASSRTGGLKTDEALGKTGAGPEGLPHSIIV
                     TAEGIEGEREVAENVPEPADGPVSAASPESAAPAKVPKKGRRAVATAPTSKKSRPASN
                     AKRKAGQQELFAEAADGKHAPARKTPSKPSKTATGTRKRK"
     gene            36006..37112
                     /locus_tag="PK589_04220"
     CDS             36006..37112
                     /locus_tag="PK589_04220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNJ18832.1"
                     /translation="MAQAKPKKNGAVAKKLVGMADVVISAAQRSKDPTFEIPIRALSN
                     VSFNPRKGLIEMGEKKQARTFFNVGMAKKFMQTMLVADALAELQQADLTTSLREIYYR
                     TKHTIQDSHENTFDTQDESDPIIEDLEVSLIALREELHVRAENSGSIVGPVVFGDDGD
                     RVDCAKLGKGGYSVPSIVEPEYLEIRRCTADFLLLVEKGTQWNRLSEDKFWRRYNCVL
                     LTGNGQPPRGVRRLARRLHEEHKLPVYVLVDNDPWGYYIYSVVKQGSINLAFESQRMA
                     IPKAKFIGLSSADPERYELPRNVGIKLNEKDVTRAKELLNYQWFQKPAWQQEIKRMLT
                     SGLKYELDALANKDFQYLTKKYLPRKLKEKDWLD"
     gene            37132..37557
                     /locus_tag="PK589_04225"
     CDS             37132..37557
                     /locus_tag="PK589_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18833.1"
                     /translation="MMQQGGRTWSIQRSVLRISVCLLLLAPALVTAQSLPPSSNQLNQ
                     QNQPGSNLQAPGTIPIPNPLAQQSAISSTASGASAPQSNTIPSSQGKSFGTIGQGLPG
                     MTSGPTLTAPMGSQDPSSRYMRPTVIPPLFCDPAINIPC"
     gene            complement(37685..38116)
                     /locus_tag="PK589_04230"
     CDS             complement(37685..38116)
                     /locus_tag="PK589_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18834.1"
                     /translation="MRSALALLLIVLTAGFTLGCSTPSASRLTGAIRYFNIRSEIAPR
                     HLIAQIGDEIRWQNLNPHPVTLRMLEDSDPALLACDKGFKQFGVIQDTVTIAPLQSVS
                     LCFLKPGTIRFNVWLDADNPQGAMTPTGTIRVEPSPVRRNS"
     gene            38257..39579
                     /locus_tag="PK589_04235"
     CDS             38257..39579
                     /locus_tag="PK589_04235"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNJ18835.1"
                     /translation="MGLTFDEARHLLSRTGFGGTPEEIRAFMAFDRPDAVKQLLTIPT
                     HKASTPPPAWIHQLPPLPRLRKFWTDRERKAFHEQLKVQGHELKAWWYRELITTRHPL
                     LERMTLFWHNHFTSSLHKVRWPAFLYQQNVLLRLHALGSFRTLLQSIAKNPAMLLYLD
                     THTNRREHPNENFARELFELFTLGEGHYTEQDIKQAARAFTGWHLDHHTGAFRMDLRH
                     HDEGQKEVFGRKGHFDGDDILSLTLEQPHVARYLVRKLWREFISEQPDAVAVERLADS
                     FRRNKYETVDLLKELFLTEAFWAPENRGALIKSPVELIVGTMRLFDLPFDDVTLQVVY
                     GRRLGQDLFDPPNVKGWPGGLRWITTSTLLSRTQLLQRAIRGHETGHLHGMTDMKQAQ
                     GAMWLATETTEVVQATLLPFAPVQPLTAGDDRGQAVHHLVLDPVYQLK"
     gene            39587..40798
                     /locus_tag="PK589_04240"
     CDS             39587..40798
                     /locus_tag="PK589_04240"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNJ18836.1"
                     /translation="MTTWSIHRRTFLEAAMALPLLWLRPGWLSLLRAADTSNFSERSH
                     KRERILLLVELQGGNDGLNTVIPYDDAAYYRARPQLAIPRDQVRQLTPKLGLHPALSP
                     LMPLWERKELAWLQGVGYPKPNRSHFRSIEIWDTASDSEEVRDKGWLSGLFAQYPLPA
                     GFTAEGVLLGKGDAGPLSGGTTRTMALHDPAQLFRQAGALKPLSASTTNRALAHILEV
                     RREISQAAIDVQQRVQEAPPFAMDFPTHKFGKQLEVAARLIAAKLPVAVIKVTHGSFD
                     THAGQLSTHHRLLEELTQGLMAFRAAMEQRGLWSQVLVMTYSEFGRRVGENASHGTDH
                     GTAAPHLFMGGRVRGGLYGAPCSLTDLEGGDLKYTLDYRSLYATVIEQWWGLPAVSFG
                     GGRYPVVDCLT"
     gene            40866..41231
                     /locus_tag="PK589_04245"
     CDS             40866..41231
                     /locus_tag="PK589_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18837.1"
                     /translation="MLRELLRTGVAVALLVSVTLLPALTSRIQAEEGGASNPSSVTQS
                     PAPRPGALQQTPAPVEPSLPLLMAEGSNSCEIVSCGMGIKQTCQITCPADKTPKCSCD
                     CERSFGPMCMDYKANCRCE"
     gene            complement(41247..41447)
                     /locus_tag="PK589_04250"
     CDS             complement(41247..41447)
                     /locus_tag="PK589_04250"
                     /inference="COORDINATES: protein motif:HMM:NF015821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HNJ18838.1"
                     /translation="MSAIQTSPHRCPECHQATAWKDNPWRPFCSERCQLIDLGAWAGE
                     QYRVPGPSLSVGGTESFSPDSE"
     gene            complement(41453..42154)
                     /locus_tag="PK589_04255"
     CDS             complement(41453..42154)
                     /locus_tag="PK589_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HNJ18839.1"
                     /translation="MPTLSRAWCSLALASALTACAAPSSQGLRQAVTPIPDCCRASHS
                     DTRQQAIVRTAVNLVGATTIESQGRRISYDCAGVTRAIYLAHGIDLYSDGSADGRTNG
                     VGLIYNHVRKNGRLHRGPVVQAGDLVFFDNTWDFNGDGLVNDPLTHVGIVEQVEQDGT
                     IVFISRVAGAIERYRMNVAHPHIHRTADGRLLNDYMRRKHWRDGEHTAYLTGELFAAF
                     GTRVIDSPSIPPAPR"
     gene            complement(42334..43986)
                     /gene="pgm"
                     /locus_tag="PK589_04260"
     CDS             complement(42334..43986)
                     /gene="pgm"
                     /locus_tag="PK589_04260"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HNJ18840.1"
                     /translation="MAQHPRAGQPAQPEQFVNLARLEQAYYQETPDPADVQQRVSFGT
                     SGHRGSSLKRTFNEAHILAITQAICEYRIKAGTTGPLFVGKDTHALSAPAQRSALEVL
                     AANGVEVRLDKDDGYTPTPVMSHAIIQTNRERTSGFADGIIVTPSHNPPEDGGFKYNP
                     PHGGPADTDVTKAIENRANELLAANLQGVKRMPYEQAIRAVSTTRYDFAGTYIADLAQ
                     VVDLDRIKAANLRLGVDPLGGAAVAYWRPLAERYGLNLEIVNASVDPTFRFMTLDWDG
                     KIRMDCSSPYAMASLITLKNRFDLAFGNDTDTDRHGIITPGAGLMNPNHYLAAAISYL
                     FTHRPGWKSSAAVGKTVVSSSIIDRLVRSLKRRLVEVPVGFKWFVPGLSDGSLGFGGE
                     ESAGAAFLRRDGTTWVTDKDGIIMNLLAAEMLAVTGKDPAQLYGELTAQLGEPVYERI
                     DAPATRAQKAALQKFSPQQVRSKELAGEPIIAMLTEAPGNKASIGGLKVTTANGWFAA
                     RPSGTEDVYKLYAESFKGQAHLLQIQEDAQALIKQVFSSAGA"
     gene            44143..44409
                     /locus_tag="PK589_04265"
     CDS             44143..44409
                     /locus_tag="PK589_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNJ18841.1"
                     /translation="MPNITLLHSPTCGACPSAKRLWKELRVKYSFSYREVDITTPDGQ
                     ELANRHSVRAVPATIIDGRLTFVGVPPRQSAEKALQLKMKPQGA"
     gene            44422..44586
                     /locus_tag="PK589_04270"
     CDS             44422..44586
                     /locus_tag="PK589_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18842.1"
                     /translation="MTVPDDDDFELPILPMIDKGPPPECGTCGDPMKFIDGDWACVDC
                     NGELLGPETG"
     gene            44683..47913
                     /locus_tag="PK589_04275"
     CDS             44683..47913
                     /locus_tag="PK589_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNJ18843.1"
                     /translation="MLRLVTGPFHPTLETRLVQDLRALKSKDRLASLAIIVPSDQLRR
                     SLKRLLILQEKLSLLNVHILSFHQLALHVDRERRTAEGAAPGRQMDLVSDVFFEHLLR
                     HIGQRRMPHTESLRLSQLPHGAWPALWASLRDLKDATVDSAVAVRAVEEGQFAPEEGE
                     KLKGLFTLYAAVREGSAALGVGSPDDLASLVTPFVPTSPFLRGLHELWYYGSYDLTQT
                     QLTLLESLAATLPVTVYFPVDAQPAYGFARQFLERHLYPMAGTSDASMLDSPEPSGSD
                     GTRANVSVEVRNAAGIDDELTLVCKQILTLVETHGYRFEEIGVVGRTLVPYQSTLVRL
                     FDQHRIPFTSTAAVPLLREPAMKTLLHLARLKGNGLHRSAMLDVITSPWNRRLTAPQG
                     GEPPRPDLWRLAVHALGITKGEEEWRRLAQLGRLEAWAGTGDESSEDPLDALSIDGPQ
                     LRLLWDCVSPLIDGVTGLPESGGYGGLTDAFCALAEDHLSIPLDMSDSIDAATQSQGI
                     TDVTEALVGALAQLRELDRLDVRITWQEWTDTFEGILERTTYALTSPSQQGVQVLDAM
                     AARGLGFRALFVVGMNEKMFPRYIHEDGFLRDSHRLVLSETLGYKIDQKLQGYGEEAL
                     LFELLRASARERLYLSYQRADAAGRPLAPSGYLDGMGVVPHAADAQSLFAVPRRWKDR
                     TALPLFIPPLLTREEHTVSSVLHGRDAASLLNAGGREGELFAHGLATQALIESGVPAL
                     SVYDGLLTDATSHWNRIRERGISPTALETYARCPFQYFSAQVLDLDAVRQTVSMELSP
                     PAMGQLCHDALRLCYLALIRQGWGTAAASAAVVTAEVTRAVTQAFAAYAMTHGTGYAL
                     TWQLAQDTVRHLVEAMVRFDCEAAAASGFIPVECEIDAKGVLPDGTAGEAIPLRGRWD
                     RLDRHPVSGALRVIDYKYRANDRIDAKDRQLLQAAVRAQRLQPALYTLMAVDGPGEQP
                     HGPQPEQVEFLYLLPQGMPMVERASFPASCWQGPSGPMLSRTVQLLVDGIRSGRHFIV
                     PDTAHCDHCEFATACRKAHQPSLWRTRRSSLAGALRALRFEKVARD"
     gene            47906..51298
                     /locus_tag="PK589_04280"
     CDS             47906..51298
                     /locus_tag="PK589_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNJ18844.1"
                     /translation="MTEQSTIPDQAAREAAATTFDRNVVVIAGAGTGKTTLLVNRLLY
                     LLMRRADPLDLSRIVALTFTNKAATEMKLRLRERLRSLVSLEGRDNPATGSGTVSIAD
                     LRLRFGWTTDEIVARAEAALRDIEKAQIGTLHSFAAHLLRLYPIEAGVSPTFQTDEDG
                     SRFEEHFTAEWELWLDRELGLSGSDHGRWRLLLAAFSLEDLRQLAYSLHSDLIDLDGL
                     RAQITENGLNQVLRDWFAERKAQAARLLARYDGPKRRKIESMLATIVDLFDLLLDDGL
                     DGIHALPPDSHELLAKELGKAPNGWVEEDFEEAESLQRIANRLLKVDQGLLGQLLEVL
                     APFIRAVRKSFLDTGWLSFDGLVGKARSLLRDYPMIREQLKRDYRALLVDEFQDTDPV
                     QYEIVLYLAERLGEQSTSWRTIELESGKLFIVGDPKQSIYAFRRADIEAFDHVVERLE
                     QGGALRCELATNFRSHAQVLDLVNGVFNRLLVAQPSVQPPNVPLTAQPNRSSQFRDTG
                     VELRLVQSEEEGDLDSAAATRVEAEHLASMISRWLTPTEPGGEAAVAGTRLRPGHIAL
                     LFRKLTQAEEYLEALRRHDIQYIIDGEKHFYRRQEVIDLVNILRCIENPHDRIALVGV
                     LRSPIGGLPDSALVSLQEAQAWDYREPGPLSKWTSPHVEPLRRLYAVLARLHEAAPRC
                     PLPQVMDLVFQQLPLLELAAASIHGEQAVANLLKIRQMAADLADRPALTLNGFVELML
                     ERLREQPDEAESALSEDTLDAVRVLTIHKAKGLEFPLVILAGLHHGDGAGRGPARPQI
                     WHDWSTGTQGLDFGGLCTAGAVLVAEKARIREQAERRRLFYVGMTRARESLVLSGALP
                     KRRVRGALLDLLAQAAGAEFNGIGEGGLSVGGVGLRHTILKGEDRFPSRERPQPTMLE
                     ETAVDQALCDRWVQRDRVWEEHRSQSLIISPSDFIRKPAPLPFSNIQPRQGRGSTSGT
                     AIGTIVHRVLQYWDFTADAERQVAAISSASLAIEEQESTTRQAIVEDVQALLHIFIQS
                     PAYARLRQATVIGREMPFLIPWQDGRQILEGAIDILYRIEGKLWIADYKTDMIPLDQV
                     TGRADLYREQARLYREAVTQSLGEPVAGFEFIFLRLGVTVPV"
     gene            51314..51718
                     /locus_tag="PK589_04285"
     CDS             51314..51718
                     /locus_tag="PK589_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ18845.1"
                     /translation="MKHLGWMIMVIVTIVGCAGPRKAEVRPLHAPTGTPEAVSQQLEQ
                     GNQLLSKEDWAGARDAYLATIQADQSLAEAHYNLALALEKLGEKAEARKHYVAAANLA
                     PGNKVIWDAPPLRKYDSELTLGKKSWMDANPK"
     gene            complement(51744..52577)
                     /gene="lgt"
                     /locus_tag="PK589_04290"
     CDS             complement(51744..52577)
                     /gene="lgt"
                     /locus_tag="PK589_04290"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNJ18846.1"
                     /translation="MLPWLHAIPYPDIDPVFLRLGPLQFRWYGLMYLIGLTLAYFIIE
                     ARARAQKLPLNKDQVYDMIVYAAVGVFAGGRLGYVLFYNLSYYLENPLKIFAVWEGGM
                     SFHGGLIGTIVALILFAKRQGMTVLTIADLAAGVTPVGLGLGRIGNFINGELYGRPTD
                     VDWCMVFPAGGMVCRHPSQLYEAFLEGLLLFTVLWLITRRLPPSGTVFGSFLVGYGLC
                     RIVVEFFREPDAQIGFIVGTISMGQMLSIPMIVAGAVILAIASQRKRPLRSDSSSTAT
                     L"
     gene            52904..53155
                     /locus_tag="PK589_04295"
     CDS             52904..53155
                     /locus_tag="PK589_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNJ18847.1"
                     /translation="MSTKGREAAKIAAIIGGGAVVGAALGLLLAPKSGAETRRDVARY
                     AKKAQLQATRFSRSVKSGVSTMVERSKALVKKDDHKEAA"
     gene            53301..53389
                     /locus_tag="PK589_04300"
     tRNA            53301..53389
                     /locus_tag="PK589_04300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53335..53337,aa:Leu,seq:taa)
     gene            complement(53661..54005)
                     /locus_tag="PK589_04305"
     CDS             complement(53661..54005)
                     /locus_tag="PK589_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18848.1"
                     /translation="MTDDYARRKVQVIKEFLESQFPTPYVVEAIVPRRDPLADTKYKV
                     ILNNVVERTLLVCPAVVMDSFPTPDHLKESLVARNIIDKVKASTDSRICHDDLGTDDQ
                     NYQKPIRSVRGL"
     gene            54454..54795
                     /locus_tag="PK589_04310"
     CDS             54454..54795
                     /locus_tag="PK589_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18849.1"
                     /translation="MKVCAGEYDSRSGLESLVCTACKHRGLRSREGIIPLFRGGHEFK
                     FSYGPSTRTVTVVLSSAAVNLWGTHGVNEEQLAKLAAEWTLLCGNTKKPVQLGIPSEE
                     FADFYLYFCRK"
     gene            complement(54894..55460)
                     /locus_tag="PK589_04315"
     CDS             complement(54894..55460)
                     /locus_tag="PK589_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18850.1"
                     /translation="MSKPIPLECPRCLLANPDAAQYCECGYDFTPHFEGFPLKSSPGA
                     APRKRLFSEEMGRGSFVLGILLIWLALSVGELYRIRIELPRRPKMELGFSSLMVGMTD
                     LAAALVVGAIGWACVWIVLAAQSKRDGSLCPKRTTFVVTLIAAGLLFVTRTIDPSVLV
                     QHVMQVLFVLAIAIASYYAGCRNWLGKW"
     gene            55531..55923
                     /locus_tag="PK589_04320"
     CDS             55531..55923
                     /locus_tag="PK589_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNJ18851.1"
                     /translation="MQASTPSFRFACFGALFAAAAVAAGAFGAHALKSILDPAMLTVY
                     ETAARYQMYHALGLFVVAWLSRETHSPLAIKAGWLFCAGILLFSGSLYLVALAGIKWM
                     GALTPLGGVSFISGWACVAWTAWRAGRP"
     gene            complement(55920..56888)
                     /locus_tag="PK589_04325"
     CDS             complement(55920..56888)
                     /locus_tag="PK589_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18852.1"
                     /translation="MPNPRGCLFVVACCVVTLLTSSIAVRAEEASPPPKPTLANRLPA
                     FQDVAKANAAQLSALPAQGDALSLFKTIIGPQLGLGDAAMTIGAKGLTPTMSQELLIS
                     DLTRAANELVRGLAAWQLVQATRDLDTSGTAESRQEMHKRIEAQTAWLSDGTTLAPAL
                     ERVQTLTAAAPTEETAPLLGGAVAQIEAWALDTVHREWFRIYNWKDQVRQQRGLVRLC
                     GTWQWSIHNHQNHREEKTAVIFAPPGSVSGTSPAEIIVLGDSVYLRWETRAGVQEDSL
                     LFSGEGQRLEGTFVNSAGGWGSITGKRTATCFNDGGKPTSPRRQHH"
     gene            complement(56988..57779)
                     /locus_tag="PK589_04330"
     CDS             complement(56988..57779)
                     /locus_tag="PK589_04330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNJ18853.1"
                     /translation="MTTQTAPFSLDQIGTGTARFPSGMAIPTATDAMVDPYIRTRVSK
                     DVHVECIQFWPQDKVTYPGIVLLHDWWGMNSQITALGARLACEGYGVIIPKLYGRLGG
                     MVTANAEVAEALAAKCNEQLLLQDINTCCEYLNTTERTKRNIHGVVGFGLGATLAIRF
                     ACQRKRLRAAVAYYGKVTAPDQLTNMMSPLLYHQAEHDTWATQADADLLRSAAAQGKR
                     IEIKTYPGTTHAFCDETRPAEHNAEATAQAWDATVAFLKTCFQGT"
     gene            57951..58919
                     /locus_tag="PK589_04335"
     CDS             57951..58919
                     /locus_tag="PK589_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNJ18854.1"
                     /translation="MPLGIGLIGLGHHGSRYAKHLLHDLPEARLVAVCRRRAERGNGL
                     PDAATVPCYSDYRELIADSRVEAVVVVTPPTLNRAMCTSVAQAQKALLVEKPLATTAE
                     DARAIMQAARASGQVMMTAQTLRFDAAVSALRARQAEAGALQYLSLASRMEPHADSED
                     WGFGGRGCVLEIGIHLLDLVRVLTQDEVLTVCCEMDRVAPAGAERRMLGRLTTGSGLV
                     CVLDVSRVTSGRIGRVELIGSEGQLSADWCGQRVTRVSARHGAGEWPTAAEPTVLSTL
                     RAFVRAVRDGLPPPITIEDGKRAVEIAEACYLSASQGGRAVTVEYR"
     gene            complement(58921..59157)
                     /locus_tag="PK589_04340"
     CDS             complement(58921..59157)
                     /locus_tag="PK589_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNJ18855.1"
                     /translation="MATKAYILIKVKAGKGKSVLTALKGIAGVEQIHACFGQPDIFVF
                     INVADERALSDVVITRIHAIEGVEETDTHIVAET"
     gene            complement(59228..59866)
                     /locus_tag="PK589_04345"
     CDS             complement(59228..59866)
                     /locus_tag="PK589_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18856.1"
                     /translation="MPATPAARPGEVPDFFMRVSLAITLLSLAPLAPDIGWSQQATGE
                     TTPVMRTVAQPSRTASVYIGSVMAMLATFEDAGILPPEGTPQANRIIKAVIQFQSAFL
                     KSDHPAIRQFFTEAHRAKLGPRADEVETSFRQSGWSADTFDAVITAGQLETAWNTDGL
                     SDGFREFNIGKQDFDILAELYQQSSRAFSTQGKSFQEVYAQRRREMPGAKSE"
     gene            60092..61621
                     /locus_tag="PK589_04350"
     CDS             60092..61621
                     /locus_tag="PK589_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNJ18857.1"
                     /translation="MLAKVLSAAIVGIDAHLVDVEVDISAGLPQFSIVGLPDATVRES
                     RDRVRAALKNSGFQFPIKKVTVNLAPANIKKEGAGLDLAIALGILVAEDVIAAEAVNN
                     TVFVGELSLDGRLKPIPGALSIGVVCRHRHRIFVTAENAEEAALADGAEVLPIHTLPQ
                     AVECLRGVQPIAALLLTGDGGAASLLQEDEDFADVKGQAHAKRALEVAAAGGHNLLMM
                     GPPGSGKTMLARRLPGILPVLAQEEALETSRIHSVVGQLSRDQPLMRRRPFRAPHHSI
                     SEAGLIGGGTMPRPGEVSLAHNGVLFLDEAGEFGRATLDALRQPLEDGHVTVTRASGS
                     LKFPARFMLVAAMNPCPCGYYGDRTRDCVCSVAQVRRYRGRLSGPLLDRLDLQIEVPA
                     VPIRALGDDAPVTDSSATIRTRVMAARARQAERYRRDGIYTNAQLKPRHLKQYCALDS
                     QGRELLEQAMARLGFSARAHGRILRVARTIADLAESDSIGPAHLAEAIQYRSFDRRVE
                     L"
     gene            61618..61848
                     /locus_tag="PK589_04355"
     CDS             61618..61848
                     /locus_tag="PK589_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18858.1"
                     /translation="MRETPVIHTARVFAWWFIVGATMALAVILLQGGIREAMQAQGSV
                     WELKLVEFLTTILGGGLLGGCIALILDRIKKS"
     gene            61891..62316
                     /locus_tag="PK589_04360"
     CDS             61891..62316
                     /locus_tag="PK589_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18859.1"
                     /translation="MDIEVGSNLYRNSNGIIDIEGVPQFEVAIKEPARALLVNFALFD
                     EVGRMMAKVVDSNLTFNERRAYQLAKSPTSVSLKHEESGTVVFALELREGNRVVFSKG
                     SFHTIKGHRLDVSQTEWRIDKKHFSGKETDTKGGAVFLG"
     gene            complement(62359..64953)
                     /gene="clpB"
                     /locus_tag="PK589_04365"
     CDS             complement(62359..64953)
                     /gene="clpB"
                     /locus_tag="PK589_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNJ18860.1"
                     /translation="MDMNRMTVKLQEALQSASSLAMRRGHQGIDVEHLLLALLEQERG
                     IASSLFEQAGVSPAAVRQAADQALAKVPQVQGPGAAPGQIHLTPRLGAVLTKAEDQMK
                     ELRDEFISVEHVVLAMVEEGGVFRRLNLTRDRLLTALQQVRGNQRVTSQDPEGTYQAL
                     EKYGRDLTKLAGQGKLDPVIGRDDEIRRTIQILSRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKGDVPEGLKQKRVVVLDMGALVAGAKFRGEFEERLKAVLKEVQSAQGQVLLFI
                     DELHTVVGAGAAEGAMDAANLLKPMLARGEMHLIGATTLDEYRKHIEKDAALERRFQT
                     VLVDQPTVEDTISILRGLKERYEVHHGVRIKDAALVAAAKLSNRYIGDRFLPDKAIDL
                     VDESAARLRTEIDSLPAELDEVSRKVLQLEIEREALKKETDAGSKARLQSIEKELNEK
                     NRDLQALKTRWESEKNSVSKLRKIRQQIEEVKQNIERSERAYDLNKVAELRYGELPRL
                     ERELELEQQSLGKKQNETRLLKEEVDEEDIAAVVSRWTGIPVTRLVEGEMEKLLKLDE
                     LLHKRVVGQEQAVTAVADAVVRARSGIKDPNRPIGSFLFLGPTGVGKTELARALSVTL
                     FDDESNLIRIDMSEYMEKHTVARLIGAPPGYVGYEEGGQLTEAVRRHPFSVILFDEIE
                     KAHHDVFNILLQVLDDGRLTDSQGRTVDFKNTVLIMTSNIGSQHILEAQQAGASYETM
                     KNQVTGELRAHFRPEFLNRVDEIVVFHALGNEHLAKIVEIQLERLRARLMERRITLTV
                     TPEALQSLGRRGYDPVYGARPLKRLIQQEIETPMARQLIKGELRDGDTAVVDLKDGQI
                     CIVPTVAP"
     gene            65192..66010
                     /locus_tag="PK589_04370"
     CDS             65192..66010
                     /locus_tag="PK589_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18861.1"
                     /translation="MLTIFNSTLQLYRDALHATWRSLVRGWFVILAVIVFTLLMMLAH
                     QIARPLGMLGGFLLGAVNALLIGATLSLIEQSIKGMRTITMHDVLESMGHYFWDVISV
                     GFVLWIPTMLLDTSLRANPDSQFLVMACLLLVFILLNPAPEVIYLTRHDSPLDVLKRS
                     YDFVLDNWIEWFLPMALVLAPLGLSLFFKLSSQLGRGAGLDFIQLVLVPFTILSAWLT
                     DLGISANLTGMMALLLTPPLMVGMLLFRGHLFSALSSTSRRQRAYQSQFHDSAR"
     gene            66077..67627
                     /locus_tag="PK589_04375"
     CDS             66077..67627
                     /locus_tag="PK589_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ18862.1"
                     /translation="MSEVVYGLTCTAASAGPRIHHQGADLVVRLSIFWRLVLTYLVII
                     AVMTGVNVYALLQIRTLAGLNTEVGSHHHPEIDSAKRLLTSIYEQVQGEKKYVAVPAA
                     TFLEHFDSESKEFQQVLQGLLAHDVSEPETRLLKEIEGLQQAHLSLFQSQLAESSGHP
                     EASAGYDARREAVVGKMAAALQQYIGLHERRLAVGVNESHASSVHAEAVTRNLVLVAL
                     LFGLGMAAVASYNILRPLRRLQATIQEMGQGNFKASWDGQAPRELRELGETVKWMGTK
                     LQQLDDIKSEFLAHVSHELRTPMASIQEGTHLLLDEIPGPLTQDQRTTLRIMSDSSRR
                     LMYLISTILDLSKMESGMMEYRFVPTDLRRVADISINKIRLLADAKHVQLVIEAPVER
                     HWVRADSTRIEQVLDNLLSNALKYSPEGAVVRLQMTPRRDEGLLFVDVADAGPGIPAE
                     ELPHIFERFYQGRTKSRQVSVGSGLGLALAKKVVEAHGGRIWVESEVGKGTTVRFILS
                     LMKPGMLA"
     gene            67747..68397
                     /locus_tag="PK589_04380"
     CDS             67747..68397
                     /locus_tag="PK589_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18863.1"
                     /translation="MLQAILRKDSSLVTKCASKNTCDHVFFTRALTSLYESRESAIHN
                     FEKVIAVAPKSQLAVSSRLWLQLLQAGEVPSDQSWLRSVWNGPAIAQTQGVLSQTIER
                     TVRDLLDRELTIQQLRALQDSEAQSLEAMQRELQDQERKAELFNSRRESHRMSVEPAT
                     LQSLQRQVSDRDKKIEELTSQLEALKRIDQEMRGKTRPIKPPSSVLPSQQSEPSKP"
     gene            68406..69821
                     /locus_tag="PK589_04385"
     CDS             68406..69821
                     /locus_tag="PK589_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ18864.1"
                     /translation="MEQERILVVDDDEGLLHLLRMRLSALGFSVTPCTTGRDALVAAR
                     QETFDIAITDLRLRAEDGLVLTEELLQVQPGLPVIILTAHGSIPNAVEAMQRGAFGYL
                     TKPFDDKELKATIDKALIQLRMTREIQRLKSLVKELYGLENVIARSPAMQRLFQQVAQ
                     IADSDATILLTGETGTGKEVLARVLHANSRRAKGPFVALNCAAISEHLFESELFGHIR
                     GAFTNAMAAKRGLFQSANGGTLFLDEIAEMSLPMQVKLLRAVQEREVREVGADYTTKV
                     DVRIITATNKDLAECVKQGTFRHDLYYRISVVPLNIPALRERRDDIPLLAQHFLKQSA
                     RRSNKDVRGFTPAAMHRLIGYPWPGNVRELENAVEKAVVMSRQDMVLPELLPSAGAST
                     DIGLKPLTEAKEEFERSYLRNVLQMTGGNISRAAQFAGRYRADFYKMLKKYGLHPSML
                     KERHDLDLAEIEESAEELKDV"
     gene            complement(70206..70571)
                     /locus_tag="PK589_04390"
     CDS             complement(70206..70571)
                     /locus_tag="PK589_04390"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ18865.1"
                     /translation="MQNRYSQRVPMDCSVMFAGDNCVGEGRILDISLPGCLLESPKQM
                     TAGEYLQLRLFLPDHQAPLHVPLAAVRWVEGSKLGIEFIRTSEDEQRRLEQFVRRVPA
                     AAHPSIRRNGIVLLGATEQ"
BASE COUNT        14521 a        19847 c        21806 g        14891 t
ORIGIN      
        1 ccgcagcaag atttcctctc aaggcttgat gcgactctcg gcctatgcta agtgtaatag
       61 gtaatggatg atggtgaccc gagaggacct tttctcgtcg tcttctcacg acatcgggcg
      121 agccttgcag ttcgaaccgt aggcttcgaa ctgatccaag gatccatttt ttaccctgac
      181 atcgttgttc cgtaccttta gcctcgatag gtcttatgga cattatcgtc ttgatcttcc
      241 ttattctgtt gtcggcggtc atctccgtcg tcgaagtggg cttctattcc gtcaacgaca
      301 cgaaactccg cgccttggcc gatacgggca gcaagcgggc ggagatggcc ctgcatctgc
      361 gcaccgatcc gcaacggctc ctgttgacca ttctggtcgg cgaccggctg gtcgatacgg
      421 cgaccgcgtc gttggccacg atcattgcct tgaaccggtt cggagggcag gggcttgaag
      481 gtgtactcgg cgaggccttt gcggtgctcg tcggtatcct cacgtttgtc ctgctggtgt
      541 tcgccgatct cgtccccaag acccttgcgg cgaaatattc tgttcccgtc gtgctgaaca
      601 tggcatatcc cgcctatgca gcacagcagg tgctcacgcc gatcatgttc ttcgtcgtgc
      661 cgttgatcta caagcttacc ggcgggaagg ggctcaatgt gcccttcgtc acggaagaag
      721 agctcaagat catgctggac cagagcagca agagcggcgc catcgaggcc caggaagtga
      781 agatgatcaa gaacgtcttc cagctgaagg acattacggc ggaagactgc atgaccccgc
      841 gcatctacat gttctcgctc gactgcaatc aatacctgcg cgaagcgaag gagctgttgt
      901 tcaagtcgaa gtactcgcgc attccgctgt acgaaggcac gctggataac attatcggca
      961 tcctctacaa gaccaaggcg ttgaccgccc tggcccaggg ccataccgaa atgaagttgc
     1021 gggatatcgc acagccggcg ttgtttatcc cgcacacgaa atcggccgac gatttgatga
     1081 aacagttcca gttggataaa cgccacatgg cgatcgtcgt gaacgaattc ggcggcgtta
     1141 tgggtctggt cacgttggag gatttgctcg aggaagtggt cggcgaaatc gtcgatgaga
     1201 cggacatcac cgaagaattg atcaaacgga tcggcaagaa ccagatcctg gtgcacggcc
     1261 gcaccgaagt gcggaaggtc aacgacttct tgaaggtcga cttgggggac gatgccgtga
     1321 cgatcagcgg actggtgcaa cacgaactcg gccggattcc caaggtcggc gaggaagtgc
     1381 atatcgccaa ctgccggctc gtgatccatg aagcggatcc gcgggtgatt aaaagcgtcc
     1441 agatctacaa ggaagacaaa caccccgcgt tgccggacca gccggtcgag gagcatgtgc
     1501 cggtcaccca ggcgtcacca gatcggtaaa ctccgcttcc gtcagaatct tcacgccgag
     1561 cttctgcgcc tgatcgagtt tcgatccggg gtcgattccc gccacgacat acgccgtttt
     1621 cttactcaca ctcgacgaca cttgtcctcc ctgccgttcg accagttctt tggcctgatc
     1681 gcgactgtag gcagcgaggc caccggtaaa cacgaaggtt ttgccggtca gggtccggcc
     1741 gcctggactg gcttccgcca ccggcggagc gaccgtcaat ccccgggcga cgaggcgctc
     1801 gatcacttcc cgatttcgcg cttcggtgaa gaacgaggcc aggctctctg agatctccgg
     1861 accaatttcc cgcacgtgca gcaactcgtc ctgcgtcgct tccatcactc gggggagtgt
     1921 gccgaaatgc ttcgccagca cgcgtgcaat gtgttgcccc acctgccgaa tccctaatcc
     1981 cattaacaaa cgctccagtg agacggcctt gcttcgctcg atggagtcca tgagcaggct
     2041 ggccgatcgg tcggcgaagc cctccagggt caacagttgt tcttttgtca ggctgtacag
     2101 atccgcgagg tctttgacca ttcctgcatc aaccagctgg gccaccgttt tcttccccag
     2161 cccgtcgata ttcagcgcgc tcttcgaggc gaagtgttcg atcgcacctt tgagttgcgc
     2221 catacagacg gtctgtcccg tgcagtaaaa gtaagcgcct tcgcgcgcca ccgccgaacc
     2281 gcagacggga caatggtcgg gcatgacgaa cggcgcctgc cgctcctcac ccgggacggg
     2341 cacccgctcg gcgattgccg gaatcacatc gccggctcgc tccaccttga ccgtatcgcc
     2401 gacgcgcacg tctttacgcg ccacttcgtc ggcattgtgc agcgtcgcgc ggctgatggt
     2461 gactccgccc acctctacgg gtttgagcag cgccaacggc gtcaacgtgc cggtccggcc
     2521 cacggagacc gcgatatcct gaatgaccgt gatttccttc ctcggggcaa acttgtaggc
     2581 aatggcccag cggggactgc gcgatttgct gccgagttga tcctgccaat cacggcgatc
     2641 cagtttgacc acgacgccgt cgatctcaaa cggaaggtcg tcccgcatcg cgtcggtcgt
     2701 ctggtggaac gtgaggacct cgtcgatgga ccggcaacgc tgccgatgtt ccggaaccgg
     2761 caagccccag gcggcgagcg cttccaactc gtcccaatgt gtggcgggca tcgggccggc
     2821 gatggccatg atgtcgtaac aggtgacggt caagggacgg gaggcggtga tgcggctatc
     2881 gagctgccgc aatgatcccg ccgccgcatt gcgcggattg gcaaagcctt cttcgccgcg
     2941 ctccgtcatc cgccgattga gagactgaaa ctcatccagc cgcatgtaga cttcgccgcg
     3001 gaccaccaga tgcgccggtg gacccgtttc ggcatgaagt tgcaagggca gcgagcggat
     3061 cgtgcggaga ttgaccgtga catcttcacc gatcatgccg tccccacgcg tcgcgccgcg
     3121 cacgaagcgg ccctgatcgt agaccagttc cactgacagg ccgtcgaact tcggttcggc
     3181 tgtgtagacc ggctggtcgg tttccagctc gcgtttcatg cgctggtcga aggcgcggac
     3241 atcctcctga tcgacgatcg aatccaggct cagcatcggt ttctcgtgcg acaccttggt
     3301 gagctcactc aaaggcggcg ccccgacccg ttgggtgggc gagtctgcgg tgatcagatc
     3361 aggatgcgcg gtttccagat cgacgagttc acgaaagagg cggtcatatt cggcatcgga
     3421 aatctcggga cgatctttcg tgtaatagag atggtcgtga tgccggatct cgcggcggag
     3481 cgccgtgatc cgttgttcaa tgctgcggtc ggccatgggt ggattactca cgaatgaact
     3541 gcgcggcgaa gctgcggata tcttgtccgt tcagcacatg gaatcgccgt aaccggtcaa
     3601 tcaactgtcc cgaaaagcga ctcaacggaa tcggcaccag gcgccgtcca gaccgtgagg
     3661 cgatctgccg ccaccgcgcc ttgggaggaa ccggcgtcac cagggccacg tgtgtctcat
     3721 gtgaatgggc agctccggcg gcgatcaaac gttcctcgag cgttgtcgca aaccccagcg
     3781 cctcatccgt ccagatgtca ggtatcgggc gaggtggaaa gatgaacaga gcgccgccgt
     3841 agagtgattg cccgatgccg ggggcaatca tgttgttcaa gaacggcgtg gcataaaaac
     3901 agagtgtgga ttcctgcgca tgttcggcat accaggtggc ctgccagctg tactgatcgg
     3961 gatcggccgg cgtatcgaag aggaaaatca cggtatcgac attcccgcgt gccggaggga
     4021 tttctttgac gtagagttcc agccggttcg ggcgccctgg cttggcgcgt tggtgccagt
     4081 ggcgcaaact ttcccgaatg tcgatgccgt ctttcatcga ggtcgtaaac ttttcgctct
     4141 tggccaggtc tgcgccgatg atcgctttgg cttgatcgcg gacgtgcgtg tgaaaacttt
     4201 cgatcttgga gtcttccggc ggccaggaac attgccgatt cggattccag agataggacc
     4261 accgctggct ggtcctgcgc ggcggacgcg ggcggagcga cagcgagcgc caggtcaccg
     4321 gtgcgccctg gagccggttc gtcgcccgaa cgatctgctc atccggcagg gcgaattggc
     4381 ccagtccggc ggaaagctgc ggcagatgtt tgccttcgat ctcctgataa gcgtaactct
     4441 tggccgtttc gaggaggcga atcgcaaagt cgtcgccggc catctgtttg gcggcgagca
     4501 ccaacgtgta caagtccggc gtcaggcggc ggtcgagcag tgcatggttc cgcacgtatt
     4561 gtagatagag ctgcagcagc tgcggcgtga cccaattggg gacggtgtgt ccctcctcat
     4621 catgcgcggt ttgccagcgc gcgcgtgttt cgatcagcag ttctttgatg ccgtcgatcg
     4681 aaagatgacg gtccgagtgg acggtggcgc ggcggcgttc gtacagttcc gtcagaaacg
     4741 gcaactcgcc taacacgaag tacagcgacg ccggttccac gaggtattgc tccggtcggc
     4801 gtatgtcggc atccggttcg tgatagtcgg cccgttgctg gtaggcctgg cgaatccagg
     4861 gccagtcggc gaagtggcac aggcagagga tgcgctcata ttccagctcc agctggtgga
     4921 gctgaaaggc catccaggcg atgcgggcct gctgttgtga tccctccgcg ggggtcgtga
     4981 gcgtcggtac catggacgcg gcgaaggcgg catagggcag gcgcttgagg gcataggcgt
     5041 cgggcgacac gaaggggatt ggttcgacta ccgacgtttc ccgatcgatg taagcccgat
     5101 acatcccttc cccgatcgcg acgcgaattc ccatcacgac tgcttgacag ggatcgacgg
     5161 gcacgtagtt gaccgtcgcg ccggtctctg agtcgggctc ctgctgcaca atcacgctga
     5221 tggtcggcag atcgacgacc gcttcttcaa gcgggtgttc gaaagagggc ggcaggggaa
     5281 cggccaggca gtcatagcgg cgatcggtca gccggtcgcg cacttcctgt gccacatcac
     5341 cgctgccgtg aaggaccgga aacagttcga tgcggggtga gaggcggaag acgtcgtcgg
     5401 cggtggaaga tggtgctgcc attatcggcc gggatgtaga gggtcatcac ttccaaagaa
     5461 gaaatctccg agtccttgcg gcagggcctg atcgccgagg gcgcgttttc gacggcgtga
     5521 ctgcatcggc aggtcgaggg cttcctcgcc gagcaccttg atgagggatt cccgccaggc
     5581 cgcgtcgacc gagagcggat cggtaggatc ctgcgcgcgc cgtttcaacg cgtattgcaa
     5641 cagatggatg ccgtcgcgga ccgagtattc gagactgagt tggtgcgctt cctggagaaa
     5701 ttccacggtc agcgccaaca tctccgccgg cgcgaagggg aggtgatacc gcaggattgc
     5761 cagttcgtct tcccgcgcgg gaaaccccat ccctaaggtc ggctgcaggc gggacagaat
     5821 ataatcgggc acctcatagg tcgacgcgtc ttcgttcatc gtcacgcaac agcggaagtc
     5881 cgcatgggcc ttgatcagaa tgcctgcgat gatcgattcc acacaacgcc ggtgatccag
     5941 tagcggagcc agcgaggccc agctcttttc gttcatccgg ttgccctcgt ccagcacgca
     6001 gatgctgccg gtgaggaccg ccgtgacgag cggcgaggcg tgatagctga tggtgccgga
     6061 ttccgccaga accggcgtaa tcaggagatc ttccggacgt gtatccgcgg tgcactgaaa
     6121 cacatacaga tcctgcttgc gctcacgggc tccggccatc gccagcgtgg tttttccgat
     6181 tccgggctga ccggtaatcc gaggcgagag gggcaggtcg cgttcgtcga tgaccagcca
     6241 gcaggccagc agctgtttca ggatctcgcg actcccgatc cattcctgac ccatgctcat
     6301 gggttgagcc aggtgcagcg tgatgccttc gatggaaatg actcgagacg aggccggttg
     6361 tgcgcctgat gccatagtct ttcgtgccgg tggttcgcta gaatgggaag aacgatacgc
     6421 tcttcgaacg gtagcatagg cacggggaga gggtcaaaca ccgtgcctgc ccatttcact
     6481 ttgactttcg ctgccttgga ccgtattcta cccactttct caggctgcga atcggcagcg
     6541 catgcgccat agaattctcc ctgggactgg catggatgcc tcccatcgag agggtgaacg
     6601 aaggagctac atgatgagtg aacaacaggg acggctgaga cgcaggagcg tagttcccat
     6661 cggtctgctg atattgggca gcctgggttt gggcggatgt gtcatgtcgg agaaatatga
     6721 ggcggagaag gcgcgcagtc tcaacttcca gcggcttctc gcgcaggaag aaaaacgcag
     6781 cgcggaactg gatgcggaag tgaagcgcgg caagcgggaa ctcagcgagt acgaagcccg
     6841 caatcgcgaa ctgggcgcgc aggtcgaggc ggttcgtcag caagcggccc agatccagga
     6901 agaggcgcag gcgatgaagg aagccagcct cttggagaag cgggcgcacg aggatatgaa
     6961 acgcctgacg accattccga aggccaaaaa agcggcgccg aagaaggact ttgcggcggc
     7021 tgtggatgag gcgctcaaga cggaggtgcc gtcggacttg agcctggagc cggccacggg
     7081 cgcggcgaag gatgcgctca ctcacagtga tctgatggat ggcgccggag cgacggctgc
     7141 agcggcgacc ggcacgaccc acacggtgcg accgggtgag accttgtacc gcatcgggca
     7201 gaaatatcat gtcgccccgg atcaattgcg caagtggaac aacttgaaag ataacaccgt
     7261 cggggtgggc caaaagctga tcgtcagcca gccgtaaccg atcagccgga tcatctctat
     7321 gaccaagcag cagtattcac tgaccctgga cgaatttcga ggcctggcaa cggaagggaa
     7381 cctgattccg ttgtaccggg agattctggc ggactacgag acgccggtgt cggcctttgc
     7441 caaaatcgac caggggccga ccgcctatct gctggagagt gtggccggcg gagaaaattg
     7501 ggcgcgctat tcctttttgg gaagcggctc ttcagcggtc atccatgaag agaagggcga
     7561 cctgattctg actcgcggga agaaacgcct gaagattcag agccgcggca acccgctgga
     7621 gcgcgtgcgt gaattgatgg aggagtaccg gccggtgacg gtgcccggac tgccccgatt
     7681 tgtcggcggc gcagtcgggt atttgagtta tgacgtggtg cggacctttg aggagctgcc
     7741 gtccctccgc aaagacagcc tggggatgcc ggaagtggcg tttctgctga ccgacaccct
     7801 gctgatcttc gacaacgtgt cgcagaagat caaagtggtg gccaacgcct atctcgaatc
     7861 gacgaccgat cgcgccgtcc gcgaggccta tcgacatgcg accgcgcgga tcgacaagat
     7921 gatcgcccgt ctcaagcgtc cggtgcggca gccccgggtg aagcgccgcc ggaagcccat
     7981 cacgttcacg cccaatatga atcgtgcgga ttttgagaag atggtcacgc ggaccaagga
     8041 atatatccgc gccggggata tcgtccaggc cgtcctgtca caacggtggg agacgcagat
     8101 ccatacgacg ccctttcagc tctatcgcgc gctgcgcgtc gtcaatcctt cgccgtatat
     8161 gtattatttg cgcgtggccg gagtcgaatt ggtgggatcg tcgccggaaa cgctggttcg
     8221 ttgcgaagac gggcagatct ccttgcgccc tatagcgggc acgcgtcgtc gcggcaactc
     8281 tcccgatgaa gatcaggacc tggctcggtc tctgctcgcg gatgaaaagg aacgggcgga
     8341 acatgtgatg ctggttgatc tcggccggaa cgacgtgggc cgggtggcgg ctcgcggatc
     8401 cgtgaaggtg gattccttga tgcaggtcga acggtattct cacgtcatgc acatcgtgtc
     8461 gcaggtaacc ggacaattgg aaaagggcaa atccgtctac gacgtgacgc gtgcctgttt
     8521 cccggccggc accgtgtccg gcgcgccgaa gattcgcgcg atgcagatca tcgaagaatt
     8581 ggaaccgacg aggcggggac cttacgccgg agccgtcggc tatttcggat tctccggcaa
     8641 tatggacatg tgcatcaata tccggacggt cgtgatcaag ggccggcagg cgtacattca
     8701 agccggcgcg gggattgtcg ccgattcggt tcctgaacat gagtatgaag agacgtgtaa
     8761 taaggcccgg gcgatgatga aagccatcga actcgccgag cagggactgg aataacagcg
     8821 tgaacacgca cgcgtgaaac cgcggaacga caagcaggca ccatgctctt aatgatcgat
     8881 aattacgact cctttaccta caatctcgtg cagtatttcg gcgagttggg tgaagacgtc
     8941 gtcgtttacc gcaacgataa gatttccatc ccggagatgg aatccttgaa gccgagccgg
     9001 ctggtcattt cgccgggccc ctgcaccccc agtgaagccg gcgtgtcggt tgaggccatc
     9061 aagcacttcg gcggcaagtt gccattgctg ggcgtgtgcc tgggccatca atcccttgcc
     9121 gtcgcgttcg gcggggaggt gattcgtgcc gagcgtctca tgcatgggaa gacctcgatg
     9181 gtgcgccacg acgggcggac cttgtttcgt ggtctgccga atcccttcga agccacgcgg
     9241 taccattcgc tcatcgtgaa ccggaagaat ctgcctgatt gtttcgagat cagcgcggaa
     9301 accgccgagg gcgaaatcat gggcatgcgg cacaagacgc tgggcatcga aggggtgcaa
     9361 ttccatcccg aatcgattct caccaccgcc ggtaaggagc tgctgcgcaa cttcctcaag
     9421 ttatagcgtg agggccggtg cgccgcctcc catcgtgtta tacccatgat caaagatgcc
     9481 atcaacaaac tcgccgagcg ggctgacctg acggagcagg aagctgagac ggtgatgggc
     9541 gagatcatgg acggatccgc cacctgcgcg cagattgccg cgtacctcat gggcctgcgc
     9601 atgaaaggcg agacggtgga agagatcgcc ggctctgtct tggccatgcg cgccagggcg
     9661 acgcacattc gggtcaagga ttcactcgtg ctagatacgt gtggcacggg aggcgatcgg
     9721 gcccacacct tcaacatatc gacgacggcg gccttcgtgg tcgcgggagc gggaatcacg
     9781 gttgccaagc atggcaatcg ctccgtgtcg tcgaagtccg gaagcgccga tgtgctggcc
     9841 gccctgggcg tcgccattca gctgccgcct gaacaggtgg ccgattgcgt caacgaggtg
     9901 ggcattggtt tcctgtttgc acccttgtac cacagcgcca tgaaacattg tgcccagccc
     9961 cggcaggagc tgggcattcg cacgttgctg aatattctcg gcccgctcac caatccggct
    10021 ggagcccgtc tccaggtcgt cggggtcttc gatgccggcc tgaccgagtt gctggccaag
    10081 gtgctggtgc atctcggcgc gcaacattgt tttgtggtgc acgggatgga cggcctggac
    10141 gaaattaccg tgacggatcg caccagaatt tcagagggca aggcgggcgt cgtgtcgagt
    10201 tacacgatcg accccgcaga gttcggactc gcgcgcgtca ggccgaagga tctcgtcggg
    10261 ggaaacgccg aagacaacgc cgccatcacg cgggacattt tcaggggccg caaaggcccg
    10321 aagcgcgata tcgtctgcct caacgcggcg tccgcactcg tcgcggggcg caaggcgaag
    10381 acgttacaag aagggttcga gctggctcag cggacgatcg acgcaggggc ggccatggaa
    10441 aagttggaac aactgatcgc atttaccaag aaggcctcgt gacaccgtga ttctcgaccg
    10501 aattctcgaa cacaagaaag cggaacttcg tcataagagc agccgcgggt atctggctga
    10561 cctgaaaacc agaatccgcg acgcaggtcc gacgctgggg tttgcggtca cgctcgacgc
    10621 gcgacgcacc gccgacaggc cggcgctcat tgcggaagtg aagaaagcgt cgccgagtct
    10681 cggccttttg cggcccgagt tcgagcaacg cttcgagccg gtcaagattg ccgaagcgta
    10741 ccgcgagcat ggcgcgtcgg cggtgtccgt gctgacggac aaggattttt tccaaggcga
    10801 cctggcgtat ttggccgatg tgaagcgcac agtgggcctt cccgcgctga acaaagaatt
    10861 catggtggcg gacattcagt tttacgaggc tcgggcctat ggcgccgatg cagtcctgtt
    10921 gattgtggcc ggtctggaaa aacgccagtt gatcgacttt tcggcattgg ccaaagagct
    10981 gtctcttgat gtgctcgtcg agacgcatca tgagcgcgaa ctcgacacgg tgctcgaatg
    11041 gttgccggac gtacggctca tcgggatcaa taaccgcgat ctcaagacct tctcgacaga
    11101 cctcggcgtg acccttcgcc tggcgaaacg gattcccgcc gacaagatga tcgtcagcga
    11161 aagcggaatc cacacacgca aggatgttga gcggttggtc gaagccggcg tccacgccat
    11221 gctgatcggc gaatcgttga ttcgcgcgca ggataccggg gcgaagattc gcgaactgct
    11281 gggtgatccg ccaaccaagg agcaatcatg aggacgctgc tgttgttcgg tctgatggcg
    11341 atgggattga cggtggccgg ctgcgtaaat caggaaattc atgaatactc tcccaagtgg
    11401 gattcctgga tgggcagcac gaaagacgac cggatcaaag agatgggcat ccccacgcgc
    11461 tgtcattcgt ttaaagacgg gggcgaagtg tgcgagtgga tgatccctca gcaggatggg
    11521 cgacaggatt tgatcggcct gactttcaat cccaaggggc aagcctgcca gtggtcctat
    11581 cgcggatttt acgggatgca aaaaagtcag acgagttgtt gacgcatgac cgtgaaggta
    11641 aaaatctgcg ggttgacgaa cgcggaggat gcagcggtgg ctgtcgaggc cggcgctgac
    11701 gccgtcgggt tcgtcttcca caagaagagt ccccgctgtg cggaaacgga ggccgtgaag
    11761 gcgatcgtca aggaattgcc tcccttcgtc ctgccgatcg gcgtcttcgt gaatgaagag
    11821 gccaaggtcg tgcgggacat catggatagc tgtggtctgg ccctggcgca actccacggc
    11881 gacgagaccg cggcctactg cgaatcgttg ggacggccgg ttctcaaagc catccgcctc
    11941 agagatcgtc gatcgttcct ggcgttggca gaatttcagg gccgcgccgg tgtgcgcggt
    12001 tttttagtgg atgccttttc tcccgacgcc tatggcggca cggggcaggt ggccgattgg
    12061 tcattggccg ccgaagctgc atcggttgcg cgcattctcc tcgccggcgg gctgactccc
    12121 gacaacgtta ttcaagccat cgagcaggtt catccatatg gcgtcgacgt cagcagcggc
    12181 gtggaagcga agcccggaaa aaaagatcat gcaaaagtcc gggcgtttct ggaagccgtg
    12241 aagcggctcc cgcgataata gttcccttcg ttgtgggccc gaggctggcg gacgttttca
    12301 tcatcctgtt atactttcac ttttacgatt agctgaagga agacacatgg cgataccaga
    12361 tcgacatggg cggttcggac cctacggcgg gcgatatgtg cctgaaactc tgatgccggc
    12421 gttgctggag ctggaagcgg cgtatcagcg cacccgcaag gacaagagct ttcaagccga
    12481 actgaaacat tatctcacgg aatatgtggg acgcccgacg ccgctgtatt ttgctcagcg
    12541 cctgacgaag cggctgggcg gcgcaaagat ttatttgaag cgggaagatc tctgtcatac
    12601 cggtgcccat aagatcaaca acgccatcgg ccaggcgttg ctcgcgcgcc gaatgaagaa
    12661 gccgcggctc atcgccgaga ccggcgcggg tcagcatgga gtggcgacgg cgacggtggc
    12721 ggcgatgttc gggctcgaat gcgaaatcta catgggcacg gaggacatgc agcggcaggc
    12781 gttgaacgtg ttccggatga ggttgctcgg ttcaaaagtc acgggtgtgg atgccggcag
    12841 ccggacctta aaagatgcca tcagcgaagc catgcgggat tggacgacca atgtacggac
    12901 cacccattat gtgctcggct ccgtcctcgg tgcgcatccc tatccgatga tgatccgcga
    12961 ttttcaagcc gtgatcgggc gcgaggctcg caaacagatt ctgtccgcgg aaggacggct
    13021 ccctgattgt ctgatcgcct gcgtcggggg cggtagcaat gccatggggt tgtttcacgc
    13081 ctttgtgccg gacaagaagg tgaggatggt cggggtggaa gccgcagggc ttggtgtgga
    13141 gagcggcaaa cacgcggctc ggttcgcagg cgggcgtcca ggcgtgctcc agggaaccat
    13201 gacttacctg ctgcaggacg agaacggtca ggtcaatctt acccattccg tgtcggcagg
    13261 gttggactat gcggcggtgg gaccggagca tagttatcac aaggaaacca accgggcaga
    13321 atacacatcg gcgacggacg atgaggcctt ggccgccttc gacttgcttg cgcgggaaga
    13381 ggggatcatg ccggcactgg aaagcgccca cgccatcgct gaggtggtga agtgtgcgcc
    13441 ccgcatgaaa aagaatcaga tcatcgtggt gaatctgtcg ggacgtggcg ataaggacgt
    13501 ccagcaggtg gcccgaatca aaggcgtgac cctgtagcag gatgttgact cgggctgccc
    13561 gctgcgtctc gcggcggcca gaggttcgac gtactgcagg agtacgcctc acctccgcgc
    13621 ttgctgcggc cgcgctggat gatcgttttg aacctcctgt gattagcttt taggacgatg
    13681 gagaggcaga ggtatatcga gatgaatcgt ttagatcaga cattcgggcg gttgaaagcg
    13741 aagggggaga aagccctcat cacctatatc atggccggtg atccttcatt gcaggacacg
    13801 gaacagttgg tgctcgaact ggagcgggca ggggccgaca tcattgaatt gggtgtcccc
    13861 ttttccgatc ccatcgcgga tggaccggtg attcaacagg cagccgaacg gggattgcaa
    13921 agcggtacct cgctccgcag gattctcgaa tccgtgacgc gtcttcgcga catgacccag
    13981 atccctatcg tcctgatggc ctattacaac aacattcatg cgttcggcga ggcggacttt
    14041 tgcagcaagg ctgccgcggc cggagtcgac ggtctgattg ttccggatat gccacctgat
    14101 gaagccgggc ctctccgcga gccggcagct gcagctgact tgcacctgat ttttctcctg
    14161 gcaccgacca gcacggcgtc acgccgggcc tacgtggcga aggagtccgg cggatttgtc
    14221 tattatgttt ccatcacagg catcactggg gctaaactga acgacatggc gggcgtgcga
    14281 gacaacgtcg ccaagattag aaagcacacc aagacacccg ttgccgtggg attcggggtc
    14341 gccacgccgg aggatgccgc gcgggttgct gagactgcgg atggcgtcat cgtcgggagt
    14401 gcgattgtcc gtcgtgttgg agaacatggg caggatggcc gactcgttca cgaagtgggt
    14461 tccttcgttc gctctctcaa agctgcgatg cagcccggat aaccgggttc gcatcggcat
    14521 ccgctcgtca tcaccttcga tagtttcgaa ctctatttgc aggccgattg caggcgactc
    14581 gtccgcatca ggcctgactc gcatccatct cgcttcctca cccgctatca attccagcaa
    14641 caatcatgca gagcggttac gctctgtcct gcaacgatcg atggttcaca tgaggagatg
    14701 ccatggccgt agctcgccgg aagatcagtc gtaccacccg taagacgaca tccacgaact
    14761 catcagtcgc cgcgcggaga aaaaccaccg tccgcaagcc agctcctatt ggagaagacc
    14821 ggctcctgcg tacgctgcag cagggtttgt tcaataccct gtcgagtgca gaaggtgagg
    14881 ggcgttcgcc aatcggggtg gctgcggtat tcgtacagtt attcctgcag ctgaccaagg
    14941 tccaggctgt cgcgctctac gtacgcgacg agcaaacccg tgagatggcc tgtctggcag
    15001 aagccggcag cagcgatccc tcagtgacgg agttgtggct ggagggactg aagaaagcgg
    15061 agcagaatgc tcaaattgtc agtggcgagc tgcatggagt tcgattacac cggatcaacc
    15121 ggctggatgg ggtggtcatg ttccgggcag ggaagtccca gcgattgccc tcgcgcaagc
    15181 tcctgtcact gctgacggcg gtggaacctt ggctggcggt cctgctggac cacgccaggt
    15241 tgacggtcaa atatgccgcc aagatccttc gcattcagca catggagcag gtgagcgata
    15301 tcttgaattc ctctctggca gaggaggaaa aattgcgacg ggccctggat gcggcggttc
    15361 ggctggttga agccgaagcc ggtgcgttgt ttctcgcatc ccccgacggg acattgagct
    15421 tgtctgctct cggcggagaa cgcgccgcag gactcacagc cttgcattcg accgttgccg
    15481 caggagtcca tcgcacgggt caggcagtgc tcatcactca gggccgtcag gatccgcgac
    15541 tggttgcagg gcagggatgg caagccgctc tctcggtcgc ttcgctcgtg tctgttccgg
    15601 ttcgcatggg cgcgcgggca gtaggggtgt tggaagtggt caatcggcgc agtggtaaac
    15661 cattcagcaa ctgggacgtg ttggagctgg cgagtctctc caatcagttc gggctggcaa
    15721 tcgacaatct gagaagggga tcaggcgtca gggcgtgacg ggcgcacaga gggtcagcgt
    15781 ttttccggcg gcatgtactt gatcatgtcg gccgccagtt tggtggccag gtccttcata
    15841 tccgggcgga tgaacagata actttcctgc acctggtggc gtgctttcca gacgatggcg
    15901 ccgttgaccg cgtccaccag tcgcatactc aagccgacgc gtgcgatgtt atcgccttcc
    15961 tctcttgtgt attcccacgc attcaccttg accaccaaca gggaatccgc gcccagggca
    16021 tgcccgatct ttatcgcgga attcttgtcc gattggccgg ttgtttccat gcgcgaaaaa
    16081 taggtcacga gtgcatcgaa agcgtctttg gtcgtctgga acgtgtcggt gacctgatca
    16141 ggcgggacga ctttttcgac ccggcggctc ttgatcagca ccttcgcgac cacttcttcc
    16201 acgtcttcac gcgcgctgtc gtaggtgccg gagatgggca gaatcgccat cgtcttcggg
    16261 taaaaacccc gcgcagccgg tccttcccac atctcctgca agcctccgca gcctgccagc
    16321 agtccggtca tcacgagaca gagcacaacg ctgatcgtgg aaaatgtgtg tcgagtcatg
    16381 gttcgtacaa taagtgtagc agattgattc actgcagacg cgaccttaga aagtcacggc
    16441 gatggatccc gaggccagtg cagcctggtc cctgaaatca tacccgccgg caatatcgat
    16501 gcgcaaggcc aagatacgta tgccgaagcc tgccgtcggg ataaagggtg ttttcgcatc
    16561 ctgcatattt ttaaacgcgc cgagtcggaa cgacagcagt tcggagagaa tcgtctgttc
    16621 tgcgccgaga ctgaggacct ggctgtacac cccgggcgtc agggtgttgt tctttgtaat
    16681 gtccacatcc gcggtcaatg tgagggagtt gtacgggttc acggcgatgc cggtccggac
    16741 ctgtggcaat agcttgaatt tgtcgccgtt cggggcatcg aacgtcggtg cgttgatatc
    16801 ctttgcgacg atgcccatcc gcaaccagga cgaagggcgg aacatggcgc ccacgtcgat
    16861 gccgagcgca ctcgagattt ttgcgcgccc gatgtcttcg aagaccttta cgtcgtcgct
    16921 cgcgccgcga atattggtgg cgccggtata ggccgcgcct tggatgacct tgccggtaac
    16981 gccgatggaa aacattcggt ccatgaacgc ataggcatag gacagcgccg cctgccggac
    17041 ttccaggccg ttcatggcga attgcccgtt gacggtaaga ttcgtgccgt tattcgtaaa
    17101 ccccaccggg ccccgcagaa agccgccgga tgtggcgaca tccgagacat tgaatcccaa
    17161 ggcatgttcg ccgaagttgc ccttgacgta gaatccgccg gaggcggctg ccgtcaggtt
    17221 cgtgcccggt ttgttcatcc gatcgatttg ctgttgcagc cgggcgatat tgggggcgga
    17281 ggtatcattc aaattgatat ggttgatgtc cttgatcgta tcgaagacgt caccgcgatc
    17341 caccacctgg ccaccgcctt gaatccgcat atcgaatttc ttgctcatcg ccatgccggc
    17401 gggattccaa taggtggcga gggagtcggt cgtcgtggct actccggctc cccccatacc
    17461 catctgccgt gatccgacaa acacaaattc cactgccgcc gcctgcagcg gcagcaagac
    17521 ggcaagcagg acggctgtgt attgaacgta ggacatgacc tgtctgttca tagattcagt
    17581 agaactccgg ctcggaagga tcgatggcgc agtctacgaa aacatgctga agccgtcaag
    17641 aaatgattgc cgcccggagc tggtcggtga cgtgtcgtct acgacgaaat aatcgtgccg
    17701 tcctgcatgg agatgatgcg atcggcctgg gcggagagtt tctcgttgtg cgtgacgatg
    17761 acgaatgttg tacctcggga gcgattcaat tgacgcatga gggcgaagag cgcgtcgccg
    17821 gtatggctgt cgaggttgcc cgtcggttca tcggccaata cgagatccgg ttgctgcatc
    17881 aaggccctcg cgacggacac ccgctgctgt tcgccaccgg ataattcgcc cggtttgtgg
    17941 tgcaggcgat cggccagacc gacctcgccg aggagcttcg tggcttcgcc gacgacatcg
    18001 gccatctcgc gtttctgaat cattgccggc aaacaagcat tttcgagcgc cgtgaattcc
    18061 ggcagcaggt ggtgaaactg aaagacgaat cctacccgtt tattgcggaa ctccgcctgc
    18121 tgttgctcgg tgagctgaaa gagattctgt ccgtcgaacg acacggtgcc tttggtgggg
    18181 cgatccagcg tgccgaggat ttgaaggagg gtgctcttgc cggctcctga cgcgcccatg
    18241 atggcgatga gctcgccgcg ctgaatccgc aagttgatgt tattcaacac cactaactcg
    18301 cggccgccca tgggaaacgt tttgtagagg tcgacaatgt caatcatgcg gtcactgcgc
    18361 tatggaatcg tggatgatca ctgcttcacg ctggtcgatg tcttggcatg ccatacgcgt
    18421 cactcatagc gaagcgcggc ggccgggtcg agttttgccg cctggagcga tggatacagg
    18481 gtggcggcaa aactaatcag gatggccgag cctgccacca acaacacatc cgatcccagg
    18541 acatggaccg gaatacggga aatgtaataa accgtgggat caaaggtcca aaaggtctga
    18601 atcaaccaga gaaaggcata ccccagcggg acgccgatgg ccgcgcctga acagccgatg
    18661 atcaggccgt tgagcatgaa aatccgcatg atgcctttac gcgtcgcacc catcgccttg
    18721 aggatcgcaa tctcgcgctg tttttccgtc acgatcatcg tcagggtgct gacgatgttg
    18781 aatgacgcca caatcgtgat taacaccaac agcaaaaaca tcatcgtctt ttccagtttc
    18841 aacgccgaga acaggttgcg gttcatttgc atccagtccc tggcccagaa ggcgaagcca
    18901 agctgctgct cgatcgaccg ggccacgtcg gcggcgcgaa acacatcggc gactttgacc
    18961 tcgatgcctg tcactgtggc gcccatgtta aaaaatttct gtgcctctcc cagttcgata
    19021 tacgcgagcg acgaatcgta ttcgtacatg ccggattgga agatgccgac cacgacgaat
    19081 tgccgaattt tcggcgtcat gctggcgccg gtcacgggtc caaccggaga gaccacatta
    19141 agcgtgtctc ccgggaaggc gccgagtcgc atcgacagct ctttcccgag gatgatgccc
    19201 ggccgcatgg cggtatccgg tccgttcggc tcgcgttcct tttccggaat gacaactttg
    19261 gtggaatgtg agagatcgtc gaggttgcca ttcaccaggt tgtgcgcgag ctcggtcaca
    19321 gtgccttcac gcgccggatc gattccgcgc aggatgatgc cttggacgcc ggatggagac
    19381 gtgagcagga cctgccggaa aataaacggc gtggccgcca gcacctccgg aacacctgcg
    19441 accttcttca cctgttcctc gtaatcgacc atcgattcct tcatacggtc gttcacgaga
    19501 atatgggccg tggtgccgag aatcttcgcc tgcacatcct ccttgaaccc cgtcatgatc
    19561 ccgacggtgc cgatcaaggc cgcgacgccg agcgtaatcc cgacgatgga cacaatcgta
    19621 ttgaaagaga tagtgcggtt acgccgcttg gcgcgcaggt agcgcaggcc gatgaagatt
    19681 tcgtagggaa gcgccatgtc tacttttcag gcctgagttg cgggaagagc acgacatccc
    19741 ggatagaagc ctgattggtg aagagcatga cgagacggtc gatgccgatg ccttggccgg
    19801 cagtcggcgg catgccgtat tcgagcgcgc gcaggaagtc ttcgtccacc cgatgggcct
    19861 cctcgtcgcc ggccgcatgt tgggcggcct gagcttcgaa gcgctcgcgc tgatcgagcg
    19921 gatcgttcaa ctcggaaaag gcgttggcga tttcgcggcc ggcgatgtac agctcgaacc
    19981 ggtcggtcaa cgaaggatcg gaatccttgc gtcgtgcgag gggggaaatt tcaatcggat
    20041 agtcagtgat aaaggttggt tgttcgagtt tcggttcgac ggtctcttca aagatctcgt
    20101 tgaggatgtt gacgagcgat tccttcgggg acacctcgac accgagccgt ttggcggcgg
    20161 cgagggcttc gtcgcgattc gtgaggaccg aggggggcag actgttcact tccaggatcg
    20221 cctgatggta ggaccagcgg cgccagggag atcccagatt gatctgcgcg ccctgatagt
    20281 cgatggtcat cgtccccaga atgtcctgcg ccaatcggct gaagagttgt tcggtcagga
    20341 tgatgaggtc ctggtagtcg gcataggcga cgtaaaactc cagcatcgta aattctggat
    20401 tgtgaatcgt cgaaatgcct tcattccgga agttgcgatt gatttcgaac acgcggggaa
    20461 atccgccgac gatcagccgt ttgaggtaca gctccggcgc gatgcggaga tacagttcag
    20521 catccagggc attgtgatgg gtgacaaagg gtttggccgc cgcgcctccg ggaatcggat
    20581 gcatcatcgg cgtttccact tccaggaagc cacgctcgat gagaaaggcg cggatcccag
    20641 agatgatgcg actgcggagt gcgaaaatct gatgcacttg cgggttcgcg atcaagtcga
    20701 cataccgttg ccgatacctg gtttcgacat ccgtcagccc atgccatttt tccggtaacg
    20761 ggcggagagc cttgctgaga aacgtgagcg tcttcgcctc gacggtgaac tcgttggtct
    20821 tggtgcgaaa gaggataccg gtcacgccga tccagtcgcc gagatccagt ccctcggaaa
    20881 tctggtgggc ctggtcaccc agcgtgtctt tcttcaaata gacctggaga cggtccgacc
    20941 cgtcttgcaa cacggcgaac gcggctttgc cgaaccggcg caacccgacg atacggccgg
    21001 cgatggtgca gccgacctgt tcttgctcga gcacctcctt gctcttttcc ccatgcagct
    21061 gcacgagctg cccggcgcgg tccttgatct cgaagcgcgt gccatagggc gcgaccccga
    21121 gctgtcggag ctggtcgagt ttcttgatgc gttgttgccg ttgatcgttt aattcatcca
    21181 tggatgggtc tctcgtcgtt cgtcgctctt cacacgtgac gcgggcacca ggcgtcacga
    21241 gaggcgggtc acagtttata cgtcgtcatc tgtcatggcc accggctggg gagtcttgcc
    21301 gccggtcatt tttctctgca gataggcttc gatgaagccg tccaggtcgc cgtccattac
    21361 cgcagacact tggccgactt cgtggccggt gcgatggtct ttgaccatct ggtacggctg
    21421 gaagacgtag gagcgaatct ggctgcccca ggcgatatct tttttctcgc cgacaatcgc
    21481 gttgaactcg gcttccttct tgcgctgttc cacttcaaag agacgggccc tcaatatttt
    21541 catggctcca ttacggtttt ggagctgaga ccgctcgttt tgacattgca ccacgatgcc
    21601 ggtcggaatg tgtgtgatgc gaatggccgt ctcaactttg ttcacattct ggccgccggc
    21661 gccgccggcc cggaacgtgt cgactcgcag gtccttatcc tcaatgacca cctctacgtc
    21721 atcgtcgagc tccggataga cgaacaccga ggcgaaagag gtgtggcggc gtttgttcgc
    21781 gtcgaacggc gaaattctca ccaggcggtg aacgccggcc tcagccttca gatagccata
    21841 ggcatagggg ccgttgaccg acaaggtcgc gctcttgatg ccggcctctt cccctgcctg
    21901 taagtccaat gtctcgatct tgaatccctt ctgctcggcc catcgaacgt acatccgcag
    21961 aagcatttgc gcccagtctt gcgactcggt gcctccggcc ccgggatgga tggcgaagat
    22021 cgcgttgttg gcatccaact cgccggagag cagcaactcg atgcggaact gggcgacggc
    22081 tttttcgaag ggctcgagtt cggccgtcag ttcctgctcc aatgcggcgt cgccagactc
    22141 caaggccaac tcgaggagcg cctcgatatc gctctgacgg cgttcggtgt cgcgccagcg
    22201 tgaaagttcc cgatccaggg cggccttgcg acgattcacc ttggcggcag cctgcgtatc
    22261 tttccagaaa tccggctggg aggttttgac gtcgatgtcg ttgagctcag cggtcatccg
    22321 agccaggtca aagatgcccc cgtagctgcg ctaactgttc accaagggtt cgcacgcggg
    22381 tccggacatc atccaacatg gcacggtcct ttctctctca ggctggtcgc ccggcaggtt
    22441 cgccgccggc tggttcggca ttaggcgaga agtagccggt caggcacaaa agcgcgatga
    22501 gtatagcaca ggcataggca aagagatcac cgtattggga gtaaaaggtc tgttggtgac
    22561 tcacgggaat ggttccacgc aacgcttctt gcgtgaagat gggagattgt tgcagcaccc
    22621 ggccatacgg gtcgatgaag ccggaaatgc cggtattggc ggcccgcgcg aaggccacat
    22681 gattctccac ggcgcgaaag accaccatgc cgaaatgttg atagggagct gacgaggggc
    22741 cgaaccaggc gtcattcgtg acggtcacca tgaagtctgc cccgttggcg gcaaactccc
    22801 gcacgagatt ggggaagatc acctcataac aaatgaccac gccgaatttt agatggaaat
    22861 ccgcgttgct ggccgcagcg gtcatgggtg gagttttttc atgcggcttg aacatcaaca
    22921 gcgtcgagcc cggaccggct tcaaaatcgc cgatgccttc caccagcttg tcgagaaaga
    22981 acagcagaga gttgtgaaac ggaatgtatt ccccgaaggg cacgagatgt tgtttatcgt
    23041 atcgcccgag gatttggcca tcggtcgcca gcaggtacgc gctattcagc aaaaacggct
    23101 tgccatccgg ataacgccgt agtgccggac tgccgaataa cagcggggcg tcagcgcgcc
    23161 gtgccatatc gttgacgatg gcccggtatt gcggctccat ctcgaagaca aagggcgtgg
    23221 cggcttccgg ccaaatgatg agatccagat tctcacccaa cccggtggtc aaccgatcat
    23281 agcgggacat ggtttcctgg cggaacgcca catcccactt ctgtgcctgt tcgacgttcg
    23341 gctgaaccag accgacggac agcatgcggg gtgtggcatg ttgcgcggcg gacaggacgc
    23401 tcgtgccgta aaagagtgcc acgccgaatc ctgaggcggc tgcaaccagt gagggccagg
    23461 gaacggaacg gatttgaaat ccccgatagg ctttgaaact ccaaatgagt gtttccgcca
    23521 gcgcggcatt gaccagcacg agcaggaacg acaccccata cactccaaaa tggtcggcga
    23581 attgaatgat ggcgagccat tggtactggg aataaccgaa cagtgcccag ggtaacccgg
    23641 agagaaaata ggtccggatc aattcgagcg tcacccacag gaagggcgcc ggcaggaatc
    23701 cgagcgtagg gaaggctatt ctgatccagg acagcgcacc ggcatagaca gccagataga
    23761 ggccgagata caccgtgagc aataacatca ccaggacggc aatcggcagc ggcaccttgc
    23821 catacacatt catggcggtg atcacccaaa acatggcgcc ggtaaaggcc agggtaccgg
    23881 cgagccatcc cacccggaag gctcgccgtg gcgaggcata ctccacggcg atgtgcagag
    23941 ggacgagggc aaaccaggcc acgatgccga ggtcgaatgc cggaaatgac agggggtaga
    24001 gaaaaccgct cgcgcaagca agcagaatga ggcgtgcgcg ggagatggtc attagccgta
    24061 acttaacgaa agccttgagt agattcaaca gtcctgtttg ggcgaatgcg tttgtatcgc
    24121 cttgatctgc agttcaccac tccgtggatt accgcccggg gcgtccgcct ggtggagcga
    24181 agcgaccagg aggagaaggg gaggcaggcg attgcggctg cgggatagcc atgcctttgg
    24241 gctgtagcgg gagcagaggg gccggtgtga gcagattcgg cggggtgggc gtaccgaaag
    24301 gcgtcagcgt ccccggcgtg gctccatgcg gtccgctaaa actgtgagag ggcacgccgg
    24361 atccgagctg cgtcggattc gtattgccgt gtggatcaga gtagatggcg ctgccgtcgc
    24421 ccaagggaga gagcgtgccg gtggtgccat gtgattctcg aaacggaagg gactgcgcat
    24481 cggcgtcgca gcgtggcgag atcaatgccc acatgacgaa caacagtatt gtcgtgatga
    24541 tcgccatgca acgcctctca caaggagacc aggcacatat accgtagaca tcctaccaga
    24601 cggcgaaggt ccgatgggaa cgacacgtaa gaagagcgca ggcattcctc gatggctggc
    24661 aggccgcgcc ggtgctcgct ttttcccgtc ttgaccgatg ttttcttgga gcgtagactg
    24721 attgcatctt aggcagtacc accctttcgg caggtggtca catggtctcc cgtggcatac
    24781 atgaccgcgg caatgaccaa gccagtcttg tccctttcct ccctcaggtt cccccgcgcg
    24841 ttaatatctc ctggcaggta cgtacgagcg tcttcgtctg tgccatgccg aaggcataca
    24901 tgcagggagc gcaggtatca cctttttcaa aggaggcctc tttatgcaca gacgacaggg
    24961 atcccgtgcc gttctaccca cggcgtgccg gcatcctgcc tgtttgcccc gcgccggatt
    25021 acctgaggca ggaaggaggg tgtcatggga cgcaaatatg acctcgtgga tctaatggca
    25081 gcgatcgggt tggtggccac actattcgga gggtatctgc tcgtgacggc tgccgatgga
    25141 ttttggcagg ctccgatcac tcccaccatg agcagtgtca tcgccagcaa tgaaccatcc
    25201 accggtatgc agtatcttca accggtgtta gggcaagcga tcgtgcaaga tctgttgctg
    25261 gagcgtgatg ccggcgcagc cttgtcgacc tctgcgatgg aattgaaccg cgctgtcagc
    25321 gaatctgagc gaatctccac cacgttgtta agtccgctcg tgctggcgga attgcgcgcc
    25381 ttcggacagg acactgatca ccgcgcgcgc atgcaatatg tcatgggcaa gtcggtcgtc
    25441 aaccagacgc ggcgtggcgt ctcgagtggg atgctttccc ctgaccagta tggcggcgaa
    25501 ttcaatacca atctgatcag gacggcggaa ttgaccgggc agcggatgca cgaccaattc
    25561 gaggccacga ggcagccggc gctggggcag tcaatcatcg aggccattca agaggaagac
    25621 cggatgaccg ccacggctca ggaacgagtc ggacgggccg ttgttcaggt cactcaggct
    25681 caggaacact atgcggaagc gaaggccgac cggcagatcc agttggcgag cgcgacggtt
    25741 gcggcggtgc ggaccgatgc gttgatggac cgattggcga tcctggaaaa catgtcggct
    25801 gaccggacga tgatcgctca tcatcgggct gcgagcgcgg acgtttctcg cggctccctg
    25861 attctggctt gtgtcgggct gatcgccctg ttcgtgggtg gattggcgtt cagcacccga
    25921 aagggcgagg tggacagcat cccgctctgg aagctcgaga cattactgca gttgcatcga
    25981 tcgggaaggt aggctgatca ccctgggaat cgatgggcga ttcccagggt gtgagaggag
    26041 accattatgt cgtgggagaa ctgggctgtg gcgatcggac ttggcgcgct cctcatgatg
    26101 ttcttcggct tgatggagat gttttttgcc ggtccgcggc atcctttgga tccgtcgcac
    26161 cggaaatagc gttcagttca ggcaggacca gaggcccggc aacatcactt tggccatggc
    26221 tttaagttcg gcttccgatt gaggcgggac gccggacagg gcgatgccga agtcatggcc
    26281 tttcgaccaa cgcacagttg cgcgacggat cagcatcggg gtttcaggga tcgcctgaat
    26341 cacaatgacc accagctcca ttccgaccgt cacggcttct ctcccgcgaa cacgcccccc
    26401 ttcgatgttg aagtcttcgg tcacgccgat ggtttgaaac gcgccattcg aataatagat
    26461 cgggtattgc acgggtatgc gtctggtgcc acgttggcga tacgaagcgt tcatctcgac
    26521 cttgttcctt tcatcaggtg gtgatggctc ctcttgtgcc ccaccatgaa acctttcgac
    26581 atcctgatcg ccttttcacg ccacagcatg aacgcggcgg cgatcgtgct tcccagcagg
    26641 tcactccaca catcatggag ggacacttct cgtccaggag ctgtgccctg cgccacttcc
    26701 gtccagaggc cgaatacgcc cgttgccagg attccgatgg aagtcgcatg agtgacgggc
    26761 cacccgcggc gaagcagtcc ccacatgact aaccaggcaa ataccccgta ggccggcacg
    26821 tgtgctccat cacgccaggc ttcgggtaca aattcgatga gagaaccgcc ccaaccgacg
    26881 cccggggcgg ccacggcaag cccgaccagc aacagcatgt aactcaggat gatgggagcg
    26941 gcaaatacca tgtaggggac aactccggtg agctcacgtg actctgcgca gagtactgtg
    27001 caggacgcgg agcgcaccat cggtccttcg gccaagtggg tagtgctttg gaggggagag
    27061 cgcccgctgg agtgtcagcc tcgaggtagg gctagatcag agaaaaaacg gccgggatgc
    27121 gatggctcag ggtttcgttt caggcttctg gtgctgccac tcccgcggat tgtcagagtg
    27181 ggcctggtag gcatggccga atccgagcac caggtgggcg tcactcatgt gcagttccca
    27241 catagcaaaa tcgccgaagc caaacagcat tcgggattgt ggaaattgtc ccaggtaccg
    27301 ctccttcacc ttgtcatacg actggtggga cggcgagaga atggcgccgc taccttggag
    27361 attgaggcgt tgcaacgctg acgggttttt cccggctccg tccggctcgg caataaagag
    27421 actaatgcgt gggtctttca gcatatgttg ggtgtgcgag gcgagaccgc tcagatgaag
    27481 ataaacccgg gaccaatcgt ctcccatcag ataggggaca tgagagccga aggggtggcc
    27541 ctgtttgagg gtcaacagca cggcggttcg tacccgcgcg acaagtgcat cgcacgcggc
    27601 ctggacctct tccgtccgca tggcatctgg catcggcata ggcattgacc gccctcccta
    27661 cgaccattgt agctgctgct gatcaattgt ccagccatct gacaatggag gcagtcgtcg
    27721 gccggacgct cctggcgaat cacgctttgc gtccgggatg gacgcaagct aagatgtgcc
    27781 cctggcgata acgtagagga tgatgtgaaa aatggagacg cagaccatga gacacatgca
    27841 atggggattg gccttggcag gtatctgtgt tgtgatcggt ttgactggtt gcggcgggaa
    27901 gcaggccgaa atcaaggaag accggttgac cgtggggcgt gtgcaggggg aagtgaaagt
    27961 cggcatgtcc gcggcgcagg tggcggaact cctgggctcg ccgaacatcg tgaccaccga
    28021 tgataagcgg cgcgaggtgt ggatctatga caaggtgtcg acggatcgtg tcgatacttc
    28081 gagctcctcc tatgccggat tgatcattct gggcgccaac tccagcgacc actccagctc
    28141 ccagcgccag cgcacgctga ccatcatcat caagtatgac gaagagaaga aggtcagaga
    28201 ctttgcctat aactcgactc aattctgagc ggcctgacac aggtagcgga cacgggtggg
    28261 ctgccttcat cctgttgctt gcctgcgggc tgttccttgg cggctgcgcg gagcagaccc
    28321 agccggcgca gttttttgaa ctctcgccgg agagcagcgc caatcgggcg atgcagacgc
    28381 ggctattcga gaccggggcg gataatgaat tgttatcggc ctcggccgcc tcgctgcaag
    28441 atctcggctt tcagatagaa gagagcgtgc gagaggttgg gcttctgcgc gcggcgaaag
    28501 agcgaagcgc ccgggagtac ggtcagtatc gcaaccgctt cctcatctgg ctggtgtcgc
    28561 tggggcacct ggtgatcccc gtcgatttac atcaaaaaat tgcggcgacg attgtcatcc
    28621 gtccggcaaa ccacagtgat gcgaggcagg aagtccggat catcttctat cgggtgatct
    28681 ggaaggggga tggccaagcg gaccgcaact atattccacc cggggagcag aagatggaaa
    28741 tgatccgcga tccggaaatt tatcagcagt tctttgcgaa gctttccaag gccgtcttct
    28801 tggaacccca ttcgatttaa cggcgaggat acatgaatcg gacacaattc atcggagcgg
    28861 tgggaatcgt ggcagctgcc ctggcgctgc acggctgtgt ggctccggag ccgaggcaag
    28921 aattattcgc gccgaccgat gcgcagatga aaatccggag tgcgcagagc cgcacgttcg
    28981 aggtgactga ttctgccgtg gccatgcgcg gggtcatcgc ggctttgcaa gatttgggat
    29041 ttattatcga acgtgccaac gcaccgctcg gtctggtaac cgcggcgcgt tttgccgaac
    29101 ccaattatta tgatgtcctg ggagtgacgg tgacggtgag gccccaagaa gccggcaaga
    29161 ccatgatccg ggccaatgcg atctataaca gtaagccgat cgaggacccg aaagtttatc
    29221 agaacttttt tgccgcggtg gagcggtcat tgtttctggt gaaggactag cgcgtgaccg
    29281 gtcctcgtcg ctcaacgctg ttctcatttc gccggtctgg cggtcggccg atcgtgggga
    29341 gcctcgcgct cctagcggtg ctcgcggggg gctgttcgcc ctatgagtgg cgacatgaaa
    29401 atcaatggga atcgcgtgac cagatctggc tctccgaggc gagccaggtc aaggtacggg
    29461 cggcgcaaag ccgggtattc gacggggcgg accgggtcaa gacgattcag gccgtggtct
    29521 ctacctgtca ggatctgggg tttgccgtcg aagtgctgga tgaggctctg ggcatcgttt
    29581 cggcaaagaa gttcgatgat tacgaaccga cccgattaag ggacccttcc taccatctct
    29641 atcgagacga cgggctgctg attctcactc cacacttccg tagttgggga ccgttttacc
    29701 accggagcaa tctggttcgt ctcaccgtca ccgtgcgacc acgtggagag ggccagttgg
    29761 tcgtgcgggc cagtgcccag tattatctga aggcgattga atcgcccgaa ccctaccagc
    29821 aattcttccg gacattggaa caggcgctgt tcattcaggc gagcacattg ccgtagccgg
    29881 aacgatcatc aacgggtgca ttatcggcgg gattcggtga taggacgttt ctgtgtgccg
    29941 ttcatcatgc cgggcgcgag aaccggatcg aggttgaatg ccacactgat gcggggggct
    30001 tttccatggt aggtgttcac ggcatgaaag agccagccgg gaaacagtac acactgtccc
    30061 gacgtgggag cataagagat gctggtccct gcatcgtgca attcagtcat gctgtactga
    30121 agatacggcg ctagcatacg cggcagacac ccgcgtgggt cgtagatttg taactctcct
    30181 cccatgtctt tgttggaaga aacctccccg acatccacat agtacacccc ggcccaaaaa
    30241 ctaccggggt gggaatgggc cgcattggtg ccgccgttcc gctgcacatt ggcccagact
    30301 tctacgacat cccactccgg tcgaccagta ccctgtccgc agcgctggga taaggtggtg
    30361 acctgtttcg ccacttccaa caccggagca aacagatcgc gtagcggttc gccggcccat
    30421 tcgagcatgg acagatcgtg aggcgatgac cagccgacca cttcgttatc tcgatgggcc
    30481 gggacggacg cttcccgttc aagaatcatg gtcgagagcc gggcgttgat gcgtgtatgg
    30541 tcagcgggag tgaataccag cagaggcgtg gaaaatagag gggtgatttg tatctcgatc
    30601 tcggtcggca tggatggtgt cccatactga tctacgaccg cggatgggtg cacggtatat
    30661 ccccgttaca acgcaagtca acctagaggt ttacccaggg agagcggggg ggactttccc
    30721 ggcaaaattg gaggcagctg tggcagaaat ccgatgctgc ctggttcaag acgggggtgg
    30781 ctccttgaca gggctttgcc gtcggcctag cctgaggtga ccaggatcca tcgaactcta
    30841 taaccaggga ggctatcatg tctgttgccc gagtgacgga aatcatcgcc gcatccccga
    30901 agagttttga cgatgccatt catcaaggta ttgcccgcgc caataagacg ttgcagcatg
    30961 tcaagagcgc gtggatcgag gatcaaaaag tggatatcga ggatggaaag atcacgcagt
    31021 atcgggtggc gttaaaagtg acgttcgtgc ttaacgaata aggacgcatt atgaaaaatg
    31081 tcgagatgtc cgtcgaggga acctggttga ccatcaaggt ggatctatca aaggagttcg
    31141 gcccgtccgc gtcggggaag accaccatca tcgcgtccac agaagggaat gtcaccatca
    31201 cggggcggga ggaaaaaatc ggcctcaatg tctatcggaa aaaatagagg cgcgggtgcc
    31261 tgaccacgac tgttccgtcc atgagcggga gagacgaaga acatgcgtgt gacaacaacg
    31321 cacctgagag gggcaattcc ggctaacggt ctggattcct gtcgtcaaaa agaggacaat
    31381 accgttcagt gcgggaaccg gatcgtacca gaacccttgg gcttcgttga ccgccatgct
    31441 gagaacccag cccagtgccc tcaaggtgcc cactcccgcg attctcatcg tggacgatga
    31501 tcccgacatt gcgcttgtct tgaaagacct cctcgagcat tccggatatc aggttcaggt
    31561 cgccgtcact ggtgcggaag cgctgaccag ggcgaagacg gactcgttct cggcagtgct
    31621 cctcgatctc atgttgcccg atatggacgg cctttcagtg ctgaccctgt taaagggcat
    31681 cgacccggca ctgccggtga tcatgctgac ggcgttcgtg gaggtagcca agaaacacgc
    31741 gtcgctcacc gagggggcct tcggctatct caccaaaccg tatgacggcg aggaattgaa
    31801 ggccctgatc aagcgggccg tcggggtcaa gcacctgtcg ctcgaagccg cttcggcgaa
    31861 acaggcgctc actgccagcg aggaccggtt tcgcgaagtc gtgcaaaccg cgccggacgc
    31921 cattgtgctt gcagatgggg aagggcgtat tttgtcatgg aacagtgcgg cggagcggct
    31981 attcgggtat cgtgcggagg aagtcctgga ccggtccctg accatgatca tgcccgaacg
    32041 atatcgcgac aatcacgagc gggctctcga acgggtgcgc accaccggcg atgtgcgctt
    32101 gaaggggacc gtcgtcacca tgcatggcct gcataaggat ggacgggaat ttcccattga
    32161 aatgtcgctg agtagttgga tttcaagcgg tacccgagtg cactgcggta ttgtccggga
    32221 catcaccgca cggaaagaag cggaagcacg cctgctccag cagcagatcg agcaacaggc
    32281 gctgttggat ctgataccag ccatggtgtg gtacaaggat tcccacaatc gcattctgcg
    32341 ggccaaccgg cgagcggccg agtctatcgg gaaaaccgtg gcggacatcg aagggcgatc
    32401 gaccgatgag ttttatccgg aggacgccga acggtatcat caggacgatt tggaagtcat
    32461 cgtgtcaggt cgaccgaaac tcggcatcat cgagccgcat cgagcgggag ggaagaagca
    32521 ctgggtgcaa acagacaaag taccctacct cgacccggcg ggaaaggtca tcggtgtgct
    32581 ggtcttcgcg caggacatca cggaccaaaa gcgtcatgaa gccgcgcttc gcgagaatca
    32641 ggatctcgtg cggatcgtga tggaagccac cggcgacggc atcgtgattg tggatggcga
    32701 cgggaatatt ctggccgtga atacacgggt ggcggagtta ttcggttatg cctcaggcga
    32761 tctgacgggg caatcactcc agcggctcat tccttcactc ttcaattttg ggacattcga
    32821 gttggcgtct gaccgcggcg cttcgacgaa gggagagtcg ttaccgctca acccgacatg
    32881 cgtcggcctg cataaggacg gcgcagagat tgccgtctcc atcagctcga cgatgctgat
    32941 gacggcaaat ggagcgcatg gcgtcgttgc cgttcacccc ctgggcggcc ccggatgaac
    33001 ctgcgctttg cgttcatcat cctggtgctg gcgttcattg cactgctgct tgccatcggc
    33061 gtcctgacgg ctattcgatg gctcaggatc cactatcccg agcgtgccaa cgccatcctc
    33121 gcggcaagcg cggtgattgt cgccggggcc gtcatcatga ccataatcga aatgaacgat
    33181 cggccgatgt tccggccgca tgatctcatc accttgcaag agccggtcgt ggcacgaacg
    33241 attcttggcg atcgcggggc gggcagcgcc acctgcgtca tcgacctgca tgaacatctg
    33301 ggcgtgttgg agattgaaat cgagcggggc gcgctgaagg cacgggtcga aagtaacaat
    33361 acctccgcgc ctgtgttttg cccgatcggc acagaagtgc gtatcgacct gaactggctg
    33421 catcgtctga ccatcactcg ccggcaaacg caggtgagcg ggccgtagct accccctgcg
    33481 cgctcttgac tgatcgaggt ggagaggcct atcgtacccg agtgggggga gttaacgccc
    33541 cgggggaata tgtacaaaaa ggggattgag tgttgaggat ggtgtgcatg cccggcttgt
    33601 accgggaagc ctgaagtcct cccgagatct ccgccgtatc tacctctcct cggtaggtgc
    33661 gtcctcctcc cacccaaacc tgccttctct tcgcaagccc ggttccataa cactgagtcg
    33721 tgtcacttcc gcgacaggtt gcgcatcagg tgcggcgacc ggaatctcct caaggcttga
    33781 ctctccgcag cgttccctgt aaacgacgag gaggatacgt atcgcgcctg tcgctgcgcc
    33841 gcgccaattc tctgaatccc ttgcttgaga ggttgaatgg ccaccaagag atcatcgcca
    33901 cgtgccgagt cttccacatc cgcgcctgcc tccgaatcca ctgaatctga atcggccgtc
    33961 tcacgaaaca aatccggcgc tgcacaaaaa tcagcgcccg cggtgacggc agtggaaatg
    34021 ggcgcgcggc agcgtgagat ctccgtgtcc gaattcttca cgaagaatcg gcacctgctg
    34081 ggattcgaca atccgcggaa ggcgttgctg acgtgcgtga aagaagccgt cgacaactcg
    34141 cttgatgcct gtgaagaagc gggcatcctt cctgacgtga cggtgaagct ggaagtggtc
    34201 gcgaacggac cggctccggt cgcaccaagc caggccaccc gctttcgcat caccgtgacc
    34261 gacaacgggc cgggcattgt ccggcagcag attccgcgtg tgtttgcgaa gttgctctat
    34321 ggctccaaat ttcaccgcat gcggatgagc cggggacagc agggcatcgg catttccgcg
    34381 gccggcatgt acggccagct cacgaccggc aagccggtga aggtgatctc ccgcaccgga
    34441 ccgcgagctg cggcccatta cttcgaagtg caaatcgaca ccaagaagaa tgaaccgctg
    34501 gtgcatgaga ataaacagat cgagtggtcg caaccgcaag gtaccgaggt ggtgctggaa
    34561 gtcgaaggac ggtatcaaaa ggggcgcgcc tccgtggatg aatggctgga acagacatcg
    34621 atcgccaatc cgcatgtgcg actgacgtat cacacgccgg aaggagagca gcgaagttac
    34681 ccgcgcacct atcatgagtt gccgccgtcg ccgaaggaga tcaagccgca tccctacggc
    34741 attgaattcg gtgcgttgct caagatgttg caggatacga agagccacac ggtcgcatcg
    34801 tttttagcca gcgacttctg ccgggtgtct cccgcgctgg ccgaggagat ctgcaaagcc
    34861 gcaaaactgt cgcccacgac caaaccgcgc gatgtcaaac cccaggcggc ggagacgctc
    34921 tacaaaacca tccaaacgac caagatcatg gcgccgccga ccaattgcat ttccccgatc
    34981 ggcgaaaagg cgattctttc gggcctctat aagcagatca agggcgagtt ttacacggcg
    35041 gtcagtcggc cgccggccgt gtatcgcggg tatccctttg tcatcgaagc ggggctggcg
    35101 ttcgggaaag ggccggaaga accttcgaag ccgcagcacg ccgcagttcc cctggcggaa
    35161 ggggaagacc aggggaatga taccgagcag gcgcgggtga tccgctacgc caatcgggtg
    35221 ccgttgttgt atcaacaatc tgcctgcgcg actttcaagg ccgtactcag taccacttgg
    35281 cgcaattatg gtgtcagtca atcccgtggc gcgttgcctg cgggaccgat ggtgatattt
    35341 gtgcatatgg cttccgtttg ggtgccgttc accagtgagt cgaaagaagc catcgccgac
    35401 tacgatgaga ttcagaagga aatcacgctg gccctgcgcg aagccgggcg gcggttggga
    35461 attttcctgc gcaggcgcga acgtgccgcc agcgaattcc ggcgccgcaa tattttcgag
    35521 ttgtacatcg aagaggtggt ggctgcgtgc aatcgtttga agggcggaaa cctgcccact
    35581 gaaaaattga aagaacagtt acacaagatc gccagttcac gaacgggtgg tctcaagacg
    35641 gatgaagcgc tcgggaaaac cggcgccggt cctgaaggtc tgccccattc cattatcgtc
    35701 acggcggaag gcatcgaagg cgagcgtgag gtcgctgaga atgttcccga gcctgcggat
    35761 ggtccggtga gtgcagcctc tcctgaatcc gctgctccgg caaaagttcc caagaaaggc
    35821 aggcgagcgg tcgcgaccgc gcctacatcg aaaaagagtc ggccagcctc gaatgcgaag
    35881 cgcaaagccg ggcagcagga gctgtttgct gaagcagctg atgggaagca cgcgccggct
    35941 cgcaagacac cctctaagcc ctctaagacg gcgacaggca ctcgcaaaag gaaataggac
    36001 agcttatggc acaggcgaaa ccgaaaaaga acggtgcagt ggcaaaaaag ctcgttggta
    36061 tggccgatgt cgtgatttcc gcggcacaac gatccaaaga tccgacgttt gagattccca
    36121 ttcgggccct gtccaacgtc tcgttcaatc ctcgtaaggg tttgatcgag atgggtgaga
    36181 aaaaacaagc ccggacgttt ttcaacgtcg gtatggccaa gaagttcatg cagacgatgc
    36241 tggtggcgga cgcattggct gagctgcagc aggccgattt gactacgtcg ctgagggaaa
    36301 tctactaccg cacgaaacat acgattcaag attcgcatga gaacacattc gatacgcagg
    36361 atgagtccga tcccatcatc gaggatctgg aagtgtcgct catcgcgttg cgtgaagagt
    36421 tacatgtgcg agcggaaaac agcggaagca tcgtggggcc ggttgtcttc ggcgatgacg
    36481 gcgatcgggt ggattgcgct aagttaggga agggcggcta ttccgtcccc tcgatcgtgg
    36541 agccggagta tttggagatc cgccgctgca cagccgattt cctgttgttg gtggagaagg
    36601 gcacgcagtg gaaccgctta tcggaagata aattctggcg gcgttacaac tgtgtgctgc
    36661 tgacggggaa cgggcagcca ccccgcgggg tgcgccgcct ggcgcgacgg cttcatgaag
    36721 aacataagct cccggtgtac gtgttagtcg ataacgatcc ctggggttac tatatctact
    36781 cggtggtgaa acaggggtcg atcaatctcg cgttcgaaag ccagcgcatg gccatcccca
    36841 aagcgaaatt catcggactg tccagtgccg atcctgagcg gtacgagttg ccgcgcaatg
    36901 tcgggatcaa gctcaatgaa aaagacgtga cccgcgccaa ggaacttctg aactaccaat
    36961 ggttccagaa gccggcctgg cagcaggaaa tcaaacgcat gctgacgagt ggcctcaagt
    37021 acgaattgga cgcgttggcg aataaggatt tccaatatct gaccaagaag taccttccgc
    37081 gcaagctgaa ggagaaagat tggttggatt agtgtgcgaa gggagcgcga tgtgatgcag
    37141 caaggcggtc gaacgtggtc gattcagcga tcagtactgc gtatcagcgt atgcctgctg
    37201 ctgttggcgc ccgctctcgt tacggcgcaa tcgttaccgc cgtcttccaa ccagttgaac
    37261 caacagaatc agccgggatc aaatctgcag gctccgggca ccattccgat tcccaatccc
    37321 ctcgcgcagc agtcggcgat ttcatccacg gcatcgggag ccagcgcgcc acaaagtaac
    37381 actatcccgt ccagccaggg caaatcgttt gggacgatcg gccaagggtt gccgggtatg
    37441 acgagcggcc cgacgctgac tgcgccgatg gggtcgcagg atccctcctc gcgctacatg
    37501 cgaccgaccg tgatccctcc gctcttctgc gatcctgcca tcaatattcc ctgctgagca
    37561 gctctgtgca gcgctggtac gtcgtgaggc tcgcgttcgt cgagcattcg cgatgtcgtg
    37621 atgccttcga atgtaatgtg gaaaggccga acagttctgg ggctgaggcg ttaggcgaag
    37681 gagttcaaga atttcgcctg actggcgaag gctcaacgcg gatagtaccg gtcggcgtca
    37741 tggcgccctg gggattgtcg gcgtcgagcc agacgttgaa gcggatggtg ccgggcttca
    37801 ggaaacagag gctgaccgat tgtaatggcg cgatcgtgac cgtgtcctga atgaccccga
    37861 actgcttgaa tcccttgtcg catgccagca acgccgggtc gctgtcttcc agcatccgta
    37921 aggtgacggg gtgtgggttc aggttttgcc accggatttc atcgccgatc tgagcgatca
    37981 aatggcgcgg cgcgatttcc gaacggatat tgaagtaccg aatggctccc gtcaggcggg
    38041 acgccgaggg tgtgctgcac ccgagagtga aaccagccgt gagtacgatg agcaggagtg
    38101 ccagcgcgga acgcatggcc gatcctacaa aatcgacgga gccgttctct actcggaatg
    38161 ttacgaggcg cagtggacgg aatggtaccg gtcaaccgaa tgagcactcc ggcgttgtcg
    38221 taaagagcga cagattgacc gtggagagca tcttccatgg ggctgacctt cgatgaggct
    38281 cgtcatctgc tctctcgcac cggcttcggt ggaacgccgg aagagattcg cgcgttcatg
    38341 gcattcgacc ggccggacgc cgtcaaacag ctcctgacca ttcccacgca taaggcctct
    38401 actccgcctc ccgcttggat tcatcaactt cctccgctcc ccaggctgcg gaagttctgg
    38461 actgaccgcg agcggaaggc ctttcacgaa cagctcaagg tacagggcca tgaactcaag
    38521 gcctggtggt accgcgaact catcacgacg cgccatccgc tcttggagcg catgaccctg
    38581 ttctggcaca accatttcac gtccagctta cataaggtcc gatggccggc atttctgtat
    38641 caacagaacg tgctgttgcg gctccatgcg ttgggatcgt ttcgcacctt gctgcagtcc
    38701 atcgcgaaga atcccgccat gcttctgtat ctcgacacac acacgaatcg ccgggagcat
    38761 cccaatgaga attttgcccg ggaattgttc gaactgttta cccttggtga aggacactac
    38821 accgagcagg acatcaagca ggctgcacgc gcgtttaccg gctggcacct cgaccaccat
    38881 accggtgcgt ttcgcatgga tctgcgacac catgacgagg ggcaaaagga ggtattcggg
    38941 aggaaagggc attttgatgg agacgatatt ctctcgctga cactggagca gcctcatgtg
    39001 gcccgctatc ttgtcaggaa actgtggcgt gagtttattt ccgagcagcc tgatgcggtc
    39061 gcagtggaac gtctcgccga ctcattcaga cgcaacaagt atgagacagt cgacctgttg
    39121 aaggaactgt tcctgaccga ggcattttgg gcgccggaga atcgaggcgc cctgatcaag
    39181 tctccggttg agctcatcgt ggggacgatg agattgttcg accttccctt tgatgatgtg
    39241 acgttgcagg tcgtgtatgg ccgcaggctg ggacaagatc tcttcgaccc tccgaacgtc
    39301 aaggggtggc cgggcggcct gcgatggatt accacatcaa ccctgttgag tcgcacgcag
    39361 ctgctgcagc gggcaatccg tggccatgag acaggccacc tgcacggcat gacggacatg
    39421 aagcaggctc agggggcgat gtggctcgcg acggagacaa ccgaggtagt ccaagcgacg
    39481 ctgcttccct ttgcgccggt gcagccgttg actgcggggg atgatcgcgg gcaggccgtg
    39541 catcacctgg tgctggaccc ggtctaccaa ttgaaatgag gctgccatga caacgtggag
    39601 catccatcga cgcacattct tggaggccgc catggcgctc ccgcttctgt ggttgcgccc
    39661 gggttggttg tcgctgctcc gggcggcgga tacaagtaat ttctcggagc ggtcgcacaa
    39721 gcgtgagcgt attctgctgc tggtggagct gcagggggga aatgacggtc tcaacacggt
    39781 catcccatac gatgacgcgg cgtactaccg ggcgagaccg caactggcaa ttcctcgtga
    39841 tcaggttcga cagctcactc ccaaacttgg tctccatcca gccctgtcgc cgttgatgcc
    39901 gctgtgggaa cgcaaggagc tggcgtggct ccaaggcgtc gggtatccca agcctaatcg
    39961 atcccatttt cgttccattg aaatctggga cacagcctcg gatagcgaag aggtgcgaga
    40021 caagggatgg ctgtctgggc tgtttgcaca gtatccgttg ccggccggat ttacggccga
    40081 aggcgttctg ttgggcaagg gagacgcggg ccccctgagc ggaggcacga cgcgcacgat
    40141 ggcgctgcat gatcctgcac agttgttccg tcaggccggt gcgctcaaac cgctgtctgc
    40201 gtctacgacg aatcgcgcac tcgcccatat tctggaggtt cggcgggaga tctcccaagc
    40261 ggccatcgat gtgcagcaac gtgttcagga ggcgccgccg ttcgcgatgg acttcccgac
    40321 tcataaattc ggaaaacaac tggaagtcgc ggcccggctg atcgctgcga agctgccggt
    40381 tgcggtcatc aaggtcactc acggcagttt tgatacccac gccggtcaac tgtctaccca
    40441 tcaccggctg ttggaagagc tgacccaagg attgatggcg ttccgcgcgg caatggaaca
    40501 acgaggactc tggagtcagg tgttggtgat gacctattcc gagttcggcc gccgggtcgg
    40561 ggagaatgcc agccacggta ctgatcatgg caccgcagcc ccgcatctct tcatgggcgg
    40621 gcgcgtcagg ggcggcttgt atggcgcacc gtgttcgctg acggacctgg aaggcggcga
    40681 tctgaaatat accctcgact accgcagtct gtatgcgacc gtgattgaac agtggtgggg
    40741 cctgccggct gtatcattcg gtggcgggcg ttatccggtg gtggactgcc tgacgtgagt
    40801 gggcgggact tgatttcgcg cggaattctt cctatcctgc ggccctctga aaggaaggtg
    40861 acgtcatgct gagggaacta ctacgaaccg gtgttgcggt ggcgctattg gtgtcggtca
    40921 cattgttgcc cgcgttgacg tcacggatcc aggcagaaga aggtggcgcg tcgaatccct
    40981 cgtccgtcac gcagtctccg gcaccccgcc caggggcatt gcagcagacg cccgctccgg
    41041 tggaaccgtc attgccgctc ctgatggccg agggatctaa ttcctgtgag atcgtgtcct
    41101 gcggcatggg catcaagcaa acctgccaga tcacctgccc agccgataaa acgccgaagt
    41161 gcagttgcga ttgcgagcgg agctttggtc cgatgtgtat ggattacaag gcgaactgtc
    41221 gctgcgaatg aggtaggctg cctcggctac tcactgtccg gcgaaaacga ttccgtccca
    41281 cccacgctca ggctaggccc tggcacgcgg tactgctctc ctgcccaggc tcccaaatcg
    41341 atcagttggc agcgctcgga acaaaacggg cgccagggat tgtccttcca ggcggtggcc
    41401 tggtgacact cgggacaacg gtgcggtgac gtctggatgg cggacaaggc tcttaccttg
    41461 gtgcaggtgg tattgaggga gagtctatca ctctcgtacc gaaggcggca aacaactcgc
    41521 cggtcaggta ggccgtgtgc tctccatccc gccagtgttt tcgtcgcata taatcattga
    41581 gcagacgacc gtcggcagtg cgatgaatat gcggatgcgc cacattcatc cggtatcgct
    41641 cgatggcgcc ggccacccgg ctgatgaaga cgatggtccc gtcctgctcc acctgttcca
    41701 cgatgccgac atgggtcagg ggatcgttga ccaggccatc cccgttgaag tcccaggtat
    41761 tgtcgaagaa caccaggtct ccggcctgta ccaccggtcc gcgatggagt cgaccgtttt
    41821 tccggacatg attgtaaatg aggcccacgc cgttggtcct gccgtcggct gagccgtctg
    41881 agtagagatc aatcccgtgg gcgagataga tggcgcgtgt cactccggcg caatcgtaac
    41941 tgatgcgacg gccctggctt tcgatcgtcg tggcgccgac caggttgacg gctgtgcgaa
    42001 cgatcgcctg ttgacgagtg tcgctgtggc tggcacggca acaatcggga atgggagtga
    42061 ccgcctgccg cagtccttgt gacgacggag cggcgcaagc ggtgagtgcg gaggcgaggg
    42121 ccaggctaca ccatgcacgt gagagcgtcg gcatactctg taagtatagc gcgtcttcga
    42181 actattctcg ccaacatcat gccagaccct cacgggctgc tccgcaaggc agtccggcac
    42241 atagtacatg aacggtaaaa gttagcatct cctgccttgc ccggcgtctt gccaagagcc
    42301 tgatcgcccg gcaggggctg attcagtagg ctgtcacgcg ccggcactcg agaagacttg
    42361 tttgatcaag gcctgcgcat cctcctgaat ctggaggaga tgggcctgtc ccttgaaact
    42421 ctccgcatag agtttgtaca catcctccgt cccgctgggg cgggcggcaa accagccgtt
    42481 ggcggtggtg actttaaggc cgccgatcga ggccttgttt cccggcgctt ctgtcaacat
    42541 ggcgatgatg ggttcccccg cgagttcctt actccggacc tgctgcggtg agaatttttg
    42601 cagtgcggcc ttttgcgctc tcgtggctgg ggcatcgatg cgctcgtaga ccggctcgcc
    42661 caattgggcc gtcaattcgc catacaactg cgccggatct tttccggtga ccgccagcat
    42721 ctccgcagca agcaggttca tgatgatgcc gtccttatcg gtcacccagg tggtgccgtc
    42781 acgacgaaga aatgccgcgc ctgcactttc ttccccgccg aatcccaaag agccgtcgct
    42841 gagccccggc acgaaccatt taaatcccac tggcacttca accagccggc gttttaatga
    42901 gcggaccaga cggtcgatga tactgctgct gacgacggtc ttcccgactg cggcactact
    42961 tttccacccg gggcgatgcg tgaacagata ggagatcgcc gcggcgaggt aatggttagg
    43021 gttcatcaag ccggcgcctg gtgtgatgat gccgtggcga tccgtgtcgg tgtcgttgcc
    43081 gaaagcgagg tcgaaccggt tcttgagggt gatgagcgag gccatggcat agggtgagga
    43141 gcagtccata cggatcttcc cgtcccaatc cagcgtcatg aagcgaaagg tcggatcgac
    43201 cgatgcgttg acgatctcca gattcaatcc atatcgctcc gccaggggac gccagtacgc
    43261 cacggcggca ccacccagcg gatcgacgcc caggcgcaga ttggccgcct tgatgcgatc
    43321 gaggtcaacg acctgcgcga ggtctgcgat gtaggtcccc gcgaaatcgt agcgcgtggt
    43381 agagaccgct cgaatggcct gctcgtaggg catgcgtttc acgccctgaa ggttcgcggc
    43441 caggagttca ttggcgcggt tctcaatggc tttcgtcacg tcggtgtcgg cggggccgcc
    43501 gtggggcgga ttgtatttga atccgccgtc ctcgggtgga ttatgcgatg gcgtgacgat
    43561 gatgccatcg gcgaagccgg aggttcgttc gcggttggtc tgaatgatcg catgggacat
    43621 gaccggggtc ggcgtgtagc cgtcgtcttt atccaggcga acctcgacac cgttcgcggc
    43681 cagcacctcc agcgcgctgc gctgggcggg agcggagagc gcatgggtat ctttgccgac
    43741 gaagagaggg ccggtcgtgc cggctttgat ccggtactca cagatcgcct gagtgatcgc
    43801 gagaatgtgc gcttcattga acgttcgttt caaggatgag ccgcggtgac cgctggtgcc
    43861 gaagctgacg cgctgttgaa catcggccgg atccggcgtc tcctggtaat aggcctgctc
    43921 gagtcgggca agattaacga actgttccgg ttgtgccggt tggccggcgc ggggatgctg
    43981 agccatggac gcctcaatta ggatggatga cgggtggatg ctgagtgccg atccgctttg
    44041 gctcagtata tacagctcgg tctcgattgt caggcgcgct cggttgcgcg gtcctcggcg
    44101 cgggtgttaa gatccgctca cgttaaggag aggggtttga ccatgccgaa tatcacctta
    44161 ttgcattcac cgacctgcgg agcctgtcct tcggcgaaac gcctgtggaa ggaactcagg
    44221 gtaaagtata gctttagcta tcgcgaggtc gatatcacga ccccggacgg ccaggaattg
    44281 gcaaatcgac attcagtccg ggcagtgccc gcgaccatta tcgatggccg gttgacgttc
    44341 gtcggcgtgc cgcctcgcca aagcgccgaa aaggcgctgc agctgaagat gaaaccgcag
    44401 ggcgcgtgag atcggagata tatgaccgtt cctgatgacg atgatttcga gctgccgatt
    44461 cttcccatga tcgacaaggg gccgccgccg gagtgcggca cctgcggcga tccgatgaag
    44521 tttatcgacg gcgattgggc ctgtgttgat tgcaacggtg aactcctggg tccggaaacc
    44581 ggttagtggc cggagcgttc accggtcgat atcgaatgaa tacgatcagc gaacaggcaa
    44641 gccgttgacc atgtcccatt caccgtggct cacttccgtt agatgctccg cctcgtaacc
    44701 ggcccattcc atcccacgct cgaaacgcgc ctcgttcaag atctccgtgc tctgaaatca
    44761 aaagaccggc tggcttcgct ggccatcatt gttccttccg atcagttgcg tcgctcactc
    44821 aagcgtctct tgatcctgca agagaagctc tccctcctga atgtgcacat cctctcgttt
    44881 caccagctgg ctctgcatgt ggatcgtgaa cggcgcaccg ctgaaggcgc agccccggga
    44941 cggcagatgg atctggtgtc agatgtcttt ttcgagcatc tcctgcggca tatcgggcag
    45001 cgccggatgc cacacacgga gtccctccgt ctctctcagc ttccgcacgg cgcctggcct
    45061 gcactttggg cgagtcttcg cgatctcaag gatgccaccg tggattcggc tgtggccgta
    45121 cgcgcggtag aggaagggca gtttgccccg gaggaggggg aaaagctcaa aggattattt
    45181 accctctacg cagccgttcg agaggggagc gcggccttag gcgtcgggtc accggacgac
    45241 ttggcttcgc tggtcactcc gtttgtgcct acttcgccat tccttcgcgg cctccatgaa
    45301 ctctggtact acgggtcgta cgatctcacg caaactcagc tcacgttatt ggaatccctc
    45361 gctgcaactc ttccggtcac ggtgtatttt cctgtcgatg ctcagcccgc ctatggattt
    45421 gcgcgacagt tccttgaacg gcatctctat ccgatggcgg gaaccagtga cgcatcgatg
    45481 ctggactcac cggagccatc tggatctgat gggacaagag ccaatgtatc ggttgaagtc
    45541 cgaaatgcgg ctgggatcga cgatgaattg acgctggtct gcaagcagat cctcacgttg
    45601 gtcgaaaccc acggatatcg atttgaggag atcggcgtcg tgggacggac cctcgtgccg
    45661 tatcaatcga cactcgtgcg tcttttcgat caacatcgca ttccctttac gtcgacggcc
    45721 gccgttccgt tgttgcgaga gccggcgatg aaaacattgc tccatctggc caggctcaag
    45781 ggcaacggtt tgcatcgttc ggcgatgctc gacgtcatca cctcgccgtg gaatcgccgc
    45841 ctgacggctc cgcagggagg cgaaccgccg cgaccggacc tctggcggtt agccgtccat
    45901 gcattgggaa ttacgaaggg cgaagaggag tggcggcgcc tggcgcaact gggacgtctt
    45961 gaggcctggg ctggtaccgg cgatgagtct tccgaagatc cgctcgatgc cctctcgatt
    46021 gatgggcctc aactgcgatt gctctgggac tgcgtctccc ccttgataga tggagtcacg
    46081 gggctaccgg aatccggagg atatggcggc ttaaccgatg cgttctgcgc gctggccgag
    46141 gatcatcttt ccattcccct tgatatgtct gattccatcg acgcggctac gcagtctcaa
    46201 ggcataaccg atgtcaccga agcactggtc ggggctcttg cgcaactccg tgagttggac
    46261 cggctcgatg tccgcatcac gtggcaggaa tggacggaca cgttcgaggg aattctggag
    46321 cggactacct acgccttgac gtctccgtcc caacagggtg tgcaggtgct tgacgccatg
    46381 gcggcgaggg gccttggctt ccgcgcgctg ttcgtcgtcg gcatgaacga aaaaatgttt
    46441 ccccgttaca ttcatgaaga cggatttcta cgtgacagcc atcgtctcgt gttgagtgag
    46501 acactgggct acaagatcga tcaaaaattg cagggctatg gggaggaagc cttactgttc
    46561 gaactgctgc gcgcctcggc ccgtgaacgt ctgtacctgt cgtatcaacg ggcggatgcc
    46621 gccggtcggc cattggcccc ctcgggatat ttggatggca tgggagtggt gccgcatgct
    46681 gcggacgcac agtctctgtt cgctgtgccg cggcgatgga aggatcggac ggcgttgcct
    46741 ttattcattc cgccgctcct gacgcgggaa gaacatacgg tgagtagcgt cttgcacgga
    46801 cgcgatgcgg cttccctgtt gaatgcaggg gggcgtgagg gtgagctgtt tgctcatggt
    46861 ctcgctacgc aagccctgat cgaaagcggc gtgccggcct tgagtgtcta cgatggcctg
    46921 ttgacggacg ccacctccca ttggaaccgg atccgcgaac gcgggatttc gccgaccgcg
    46981 cttgaaactt atgcgcgttg cccttttcag tatttctcgg cccaggtgct ggacctcgac
    47041 gcggtacggc agacggtctc gatggagctg tcaccgcccg ctatggggca actgtgccat
    47101 gatgcgctgc ggctctgtta tctggcgctg attcgccaag ggtgggggac ggctgcagcg
    47161 tccgctgcgg ttgtcaccgc ggaagtcact cgggccgtca cgcaggcgtt cgccgcctat
    47221 gcgatgaccc acgggaccgg atatgccctt acctggcagt tggcccagga cacggttcga
    47281 catctggtcg aagcgatggt caggttcgat tgcgaggccg ccgcagcgtc agggtttatt
    47341 ccggtcgagt gcgaaatcga tgcaaagggt gtcctccctg atggcacagc aggcgaagcc
    47401 attcccctgc gtggacgatg ggatcggcta gaccgtcatc cggtgtcggg ggcgctccgg
    47461 gtgatcgact acaaatatcg cgcgaatgac cggatagacg cgaaggatcg ccagcttctg
    47521 caggcggcgg tgcgtgcgca gcggttacag cctgcgctgt acacgctgat ggccgtcgat
    47581 gggcctggcg aacagcctca cggccctcag cctgaacagg tggagttttt gtatctcctg
    47641 ccacagggca tgccgatggt tgagcgtgca tcgtttccgg cctcttgctg gcaaggtcca
    47701 tccggtccga tgttgagccg aacggtgcaa ctcctggttg atggcattcg gagcgggaga
    47761 catttcatcg taccggatac ggcccactgc gaccattgtg agtttgcgac cgcctgccgg
    47821 aaggctcatc agcccagctt gtggcggact cgccgttcct ccctggccgg tgcattacgc
    47881 gcgctgcgct ttgagaaggt ggcacgtgac tgagcaatcg acaatcccgg accaggcggc
    47941 gcgggaagcg gcggccacca ccttcgaccg caacgtcgtg gtgatcgccg gagcgggaac
    48001 ggggaagacc accctgctgg tgaaccggct gttgtatctg ctcatgcgcc gggccgaccc
    48061 gctggatctc tcacgcatcg tagccctgac ctttaccaac aaggcggcta cggagatgaa
    48121 gctgcggttg cgcgagcggc tgcgatcctt ggtgagtctc gaagggcggg acaatccggc
    48181 cacagggagc gggacggttt cgatcgcaga tttacgcctg cgtttcggct ggacgacgga
    48241 cgaaattgtg gcgcgcgctg aagcggcgct gcgcgatatc gaaaaggcgc aaatcgggac
    48301 cttgcatagt tttgccgccc acctgctgcg tctctatccc attgaagccg gggtcagtcc
    48361 gacgtttcaa accgatgagg atggttctcg cttcgaagag cacttcaccg cggaatggga
    48421 gctctggttg gaccgtgagt tgggtctgtc gggatccgat catgggcgtt ggcggctgct
    48481 gttggcggcc ttcagtttgg aagatctgcg gcaattggcc tatagcctgc atagcgatct
    48541 gatcgatctc gatggcctga gggcgcagat cacggagaac ggtctcaacc aggtgctgcg
    48601 cgactggttc gccgagcgga aggcgcaagc cgcacgtcta ctggcccgct atgacggtcc
    48661 gaaacggcgc aagatcgagt cgatgctggc cacgattgtt gatctgttcg acctgctgct
    48721 cgacgatggc ttggatggaa tccacgccct tccacctgac tctcacgagt tgctggccaa
    48781 agaacttgga aaggccccga acggatgggt cgaagaagac ttcgaagagg ccgaatcact
    48841 ccagcggatc gccaatcgtt tgctcaaggt ggatcaaggg ctgctcgggc aactgttgga
    48901 ggtattggcg ccgtttatcc gcgcagtcag aaaatcgttc ctggatactg gctggctgag
    48961 ctttgacgga ttggtgggca aggcgcgcag tctgctacgg gattatccga tgattcgcga
    49021 gcagctgaaa cgggactacc gcgccctctt ggtcgatgaa tttcaggata ccgatccggt
    49081 gcaatatgaa atcgtgttgt acctggcgga gcggttaggt gaacaatcta catcctggcg
    49141 gacgatcgag ctcgaatcgg gaaagctgtt catcgtcggc gatcctaaac aatcgatcta
    49201 cgcgtttcgg cgggctgaca ttgaggcctt cgaccatgtc gtcgagcggc tggaacaagg
    49261 cggtgccctg cgttgcgagc tcgccaccaa tttccggagc cacgcgcaag tgctcgacct
    49321 ggttaacggc gtgttcaacc gtctcctggt cgcgcaaccg tcggttcagc cgccgaacgt
    49381 tccattgacc gcgcagccga atcgatcgag tcagttccgc gataccggcg tggagcttcg
    49441 actggtgcag tctgaggagg agggtgacct ggattcggcg gctgcgacca gggtggaagc
    49501 cgagcatctc gcttcgatga tctcccggtg gctgactccg accgagccgg gaggcgaggc
    49561 ggcggtggct ggcaccaggc tccgcccggg ccacatcgcg ctgttgtttc ggaagttgac
    49621 ccaggccgag gagtacttgg aagcccttcg acggcatgac attcagtaca tcatcgacgg
    49681 ggagaagcat ttctatcgcc ggcaggaagt tattgacctg gtgaacatcc tccgctgcat
    49741 cgagaatccc cacgaccgta ttgcgctggt cggtgtctta cggtcgccga tcgggggctt
    49801 gcctgattcc gccttggtga gtctccagga ggctcaggca tgggattatc gcgaaccagg
    49861 cccgctttcg aagtggacga gtccccatgt tgagccgctc cgacggttgt atgcggtact
    49921 ggcgaggttg catgaagcag ctccccgttg tccattgccg caggtgatgg atctggtgtt
    49981 tcagcagctt cctttgctcg agctggccgc ggcctcgatt catggagaac aggcggtggc
    50041 caatctgctg aagatacgac agatggccgc ggacctggct gataggccgg ccctcacctt
    50101 gaacgggttt gtggagttga tgttggagcg actccgtgag caaccggacg aggcggagag
    50161 tgcattgtct gaggatacgc tggatgccgt gcgggtgttg acgatccata aagcgaaagg
    50221 gctggagttt ccgttggtga ttttggccgg gctacaccat ggggatgggg ctggtcgagg
    50281 gccagcgcga cctcagattt ggcacgactg gtccaccggc acgcagggac tcgatttcgg
    50341 tggcctctgc acggcgggcg cggtgctggt tgcggagaag gcgcggatac gcgaacaggc
    50401 ggaacgccgc cggttgtttt acgtcgggat gacccgtgca cgggagtctc tggtgttatc
    50461 gggagccttg ccgaaacgcc gggtacgcgg tgccctgctg gatcttttgg cgcaggccgc
    50521 gggagctgag ttcaacggga tcggggaggg tgggctctca gtgggaggcg tcggtttgcg
    50581 acacacgatt ctcaagggcg aagaccgttt tccttcccgg gaacggccgc agccaacgat
    50641 gttagaagag accgccgtcg accaggctct ctgcgatcgc tgggtgcaac gtgatcgagt
    50701 atgggaggag catcggtcac agtcgctgat catctcgcct tcggatttca ttcgaaagcc
    50761 ggcaccgctg ccgttctcga atattcagcc tcggcaaggc cggggttcga cttctggaac
    50821 agccatcgga acgatcgtgc accgggttct gcaatattgg gatttcaccg cggatgccga
    50881 acggcaggtg gcggcaatca gcagcgcatc actggccatc gaggaacagg agtcgacaac
    50941 ccgccaggcg atcgtagagg acgtgcaggc cctgcttcac atctttatcc agtcgcccgc
    51001 atatgcccgc ctccgccagg cgactgtgat cggacgggag atgccgtttc tcatcccctg
    51061 gcaggacggg cggcaaattt tagagggtgc cattgatatt ctttaccgca ttgaaggcaa
    51121 actctggatt gccgactaca agacggatat gatcccgctc gatcaggtca ccggcagggc
    51181 ggacctctat cgtgagcaag cgcgattgta ccgggaggcg gtcacccaat cgctgggaga
    51241 accagtcgct ggattcgaat tcatttttct ccggctgggg gtgacggttc cggtctgaac
    51301 acaaggagta ggcatgaaac atcttggatg gatgatcatg gtgatcgtga cgatcgtcgg
    51361 ctgtgcgggt ccgcggaaag cggaggtgcg gccgctccac gcgccgaccg gaactcccga
    51421 agcggtgagc caacaattgg agcagggcaa ccagttgctc agcaaagagg attgggctgg
    51481 cgcgcgagac gcctatctcg cgacgatcca ggctgatcaa tcactggcag aagcgcacta
    51541 caatttggcg ctggccttgg agaaactggg cgagaaagcc gaagccagga aacattatgt
    51601 cgctgcagcc aatctggctc ccggcaataa agtcatttgg gacgctccac cgctcagaaa
    51661 atacgacagc gagctgaccc tgggaaagaa gtcctggatg gacgccaatc cgaaataaac
    51721 accttgtgcg acgatgatgc tgctcagagc gtcgcggttg agctggagtc tgagcgaagc
    51781 ggccgcttgc gttgagaggc gatggcaaga attacggccc ccgcaacgat catgggaatg
    51841 ctgagcatct gccccatgga aatcgtcccg acaatgaagc cgatctgcgc atccggctcc
    51901 cgaaaaaact ccaccacaat ccgacaaagc ccgtagccga ccaggaaaga tccgaagacg
    51961 gttcctgatg gcggcagacg gcgggtgatc aaccacagca ccgtaaacag caacaggccc
    52021 tccaggaacg cttcataaag ctgtgaggga tggcggcaga ccatcccgcc ggctggaaat
    52081 accatacacc agtccacatc agtggggcgg ccgtacagct caccgttgat aaagttgcca
    52141 attcgcccca gtccgaggcc gaccggggtc acaccggcgg ccaggtcggc aatcgtgagc
    52201 accgtcatgc cttgccgttt tgcaaacagg atcagcgcca cgatggtacc gatcagccca
    52261 ccgtggaagg acatgccgcc ttcccagacc gcgaaaatct tcagtggatt ctccaggtaa
    52321 taggacaggt tgtagaacag cacatagccc agccgtccgc cggcaaacac gcccaccgcc
    52381 gcatagacga tcatgtcgta gacctgatcc ttgttcagcg gcagtttctg agccctcgct
    52441 cgcgcttcga tgatgaagta ggcgagcgtg agtccgatga gatacatcag gccgtaccat
    52501 cggaactgca atgggcccaa tcgaagaaat acggggtcga tatccggata gggaatggcg
    52561 tgtaaccagg gcagcataga aggactccac atagtttcgc gatcttatgt gagcgtgctc
    52621 gacattgcaa ggcaagcggc gagcccgcgc caggcaaggc gtgagcggca ctcatgaaag
    52681 ttccctacgc acacctgtcg cttttctgaa acggttgtct cctgctgttg ttcacaaaac
    52741 agcacccttt atgaggcgtc tctaacgtgc agctcgcgaa tcacacaaat caggccgaac
    52801 cgcacatacg catcgtctgg cacgcttgat gctgtatagc gagatagcta ctcgccaagt
    52861 cagtgaacca tcaaccttgt tgtacgagag ggaggaagtc acgatgtcta ccaaggggcg
    52921 tgaagcagcc aagattgcag caatcatcgg tggcggggcg gttgtgggcg cggcgttggg
    52981 tttattgttg gcgccgaagt ccggtgcgga aacgcggcgt gatgtcgccc gctatgccaa
    53041 gaaagcccag cttcaggcga cccggttcag ccgctccgtg aaaagcggtg tgtctacgat
    53101 ggtggaacgc agcaaggcgc tggtgaagaa agacgatcac aaagaagccg cctaaccggc
    53161 gagtgagaaa acaggcgatc aaaccactgg tgtgcaacgg cctgtttcat atgggacagg
    53221 tcgttgcata gtaaagattc tctccagcag cttgaccctc cggtacccgg gtgttagatt
    53281 tcgccgcgac ggtttttgac gcgggagtgg tggaatcggc agacacccag gacttaaaat
    53341 cctgtgtcat accaatggcg tgcgggttca agtcccgcct cccgcaccaa aagaccggtc
    53401 agcggtgttg tctctcctcg actcgtcctc tctgaactct gctctccagc ctgattgacc
    53461 agttctcaca ctacacgccc ttcacttttt cgagcgcatc agtagattct cggtgattcg
    53521 gctgatcgga agatatgcgc gggacgaacg atggatcgtg atgcaggggg tgtcactgcg
    53581 acgcgagacg acgttccacc atcacagccg tgtaatgctg gacgggtctg tcgataaacg
    53641 ggaagcggaa cagcggtgcg ctagaggccg cgcaccgatc gaataggttt ttgataattt
    53701 tggtcgtcgg tacctagatc gtcgtggcag atccgggagt ctgtcgacgc cttgaccttg
    53761 tcaatgatgt tgcgggccac gagtgattcc ttcagatggt ccggcgtggg gaagctgtcc
    53821 atcaccacgg caggacagac cagaagggtg cgctccacca cattgttgag gatcactttg
    53881 tatttcgtat cggcgagtgg atctcttctc ggcacaatgg cttccacgac atagggcgtt
    53941 ggaaattgcg actcgagaaa ctccttgatg acttgtacct tcctgcgcgc gtaatcgtct
    54001 gtcacggtcg atggtccttc cgaattccgc ccctgtcggt ttgccgacgg caggggaagc
    54061 agcggttgtg gtttcagatc tcttgtggca cagtgccgtc ccgtagagtg ctgatattaa
    54121 gagaaaaacc gactgaaaac cagaggctgg tcccggccgg tcgcaaatcg ctgcgatctt
    54181 ctcacgactt cacagcgaat gaccgggcgg agccagggtc tgttgtggcg tctgatatac
    54241 ttgccatagt taaccatggc atcgtgtcca tgctgagctc cggtgggaat tttgtagatg
    54301 gttcaccgca tcgctgaggt cactgtgcag gaaaacgacg gttctggagc ggcaggcgat
    54361 ccggcggcac catccacacc agtttcatcc ctcgcacaga tctcgaaaga ctcagatggg
    54421 gcccgttgcc cggttcagaa gtgtgacggc cgcatgaagg tgtgcgccgg cgagtacgat
    54481 agccgcagcg ggttggaatc tctggtctgc acggcatgta agcaccgggg attgcgttcg
    54541 cgcgagggca tcatcccatt gtttcgggga gggcacgaat tcaaattcag ttacgggcct
    54601 tccacgcgca cggtcaccgt ggtattgagt tccgcagcgg tgaatttgtg gggcacacac
    54661 ggggtgaatg aggaacaatt ggcgaagctt gcggcagaat ggacgcttct atgcggtaat
    54721 acgaagaaac cggtgcaatt aggaattccg tctgaagaat tcgccgactt ctatctctac
    54781 ttctgcagaa agtaacgcca gtttagcatc cagcgtagtc acctccaccg cagctcattc
    54841 tcctacagtt ttttcgaata gcctgaggcg ggtgctgcac gtcggttcat ctatcaccat
    54901 ttccccagcc aatttctgca tcccgcataa tacgacgcaa tcgcaatagc caggacgaag
    54961 aggacctgca tgacgtgttg gaccaacacg ctggggtcaa tggtccgtgt cacaaaaagc
    55021 aggccggcgg cgatgagggt cacgacgaag gtggttcgct tggggcagag gctgccgtcg
    55081 cgtttgcttt gcgcggcaag gacgatccag acgcaggccc agccgatcgc ccccacgacg
    55141 agagccgcgg ccagatccgt catgccgacc atcaaactgc tgaagcccaa ttccattttc
    55201 gggcggcgag gcaactcgat acggatgcgg tagagttcgc cgaccgacag cgccagccag
    55261 atcagcagaa tgccgagcac aaaactcccg cggcccattt cttccgagaa caagcgtttc
    55321 cgcggcgcag ctcccgggga ggatttcagg ggaaagcctt caaagtgcgg ggtaaagtcg
    55381 tacccgcatt cgcaatactg cgccgcatcg gggtttgcca ggaggcagcg gggacattca
    55441 agcggaatgg gtttgctcat ttgggatcgg cttcatggta aaatcgcgcg ccctgcctcc
    55501 ggccaatgat ggaggcttca cgaggtctgt atgcaggcat caactccatc gttccgattc
    55561 gcatgtttcg gggccctgtt tgcggcggcg gccgtagcag ccggcgcgtt cggcgcccac
    55621 gcgctgaaat cgattttgga tccggccatg ctgactgtat atgaaacggc ggcgcgctac
    55681 caaatgtacc atgctctcgg cctctttgtc gtagcctggc tgagtcgcga gacgcatagt
    55741 ccactggcga tcaaggctgg ttggttgttc tgcgccggga tcctcctgtt cagcggaagt
    55801 ttgtatcttg tggcgctggc aggaatcaaa tggatgggcg cactgacgcc cttgggcggg
    55861 gtgtcgttta tcagcggatg ggcttgtgtc gcctggaccg cctggcgagc gggccgccct
    55921 tagtgatgtt gacgacgcgg tgacgtcggt ttgccgccat cgttgaagca ggtggcagtg
    55981 cgtttgccgg tgattgatcc ccatccccca gccgaattga cgaaggtgcc ttccaaccgt
    56041 tgtccctctc ccgaaaagag cagactgtct tcctgcacac cggccctggt ttcccatcgc
    56101 agatagacgc tatcgccgag gacgatgatt tccgctggac tggtgcccga cacggatccc
    56161 ggtggcgcga agatgaccgc cgtcttttct tcgcgatgat tttgatgatt gtgaatggac
    56221 cattgccacg tcccgcacaa ccggacgagg ccgcgctgtt gtctcacctg gtctttccaa
    56281 ttgtagatcc tgaaccattc gcggtgcacg gtgtcgaggg cccacgcttc gatctgggca
    56341 acggctccgc cgagcagggg ggccgtttct tccgtcggcg cggctgctgt tagcgtctga
    56401 acacgctcca gcgctggagc cagcgttgtt ccgtcgctca accaggccgt ctgggcctcg
    56461 attcgcttgt gcatctcctg acgcgattct gctgtgccgg atgtatcgag gtcgcgggtc
    56521 gcttggacca attgccaggc agcgagtccc cggaccagct cattggcggc tcgcgtcaga
    56581 tcactgatga gcagttcttg gctcatggtc ggggtcaatc ctttggcccc gatagtcatc
    56641 gcggcatcac cgagtcccaa ttgcggacca atgatcgtct tgaagaggga aagcgcatct
    56701 ccctgcgcgg gaagggccga caactgcgcc gcattggcct tcgccacatc ctggaaggcc
    56761 ggcaggcggt tggccagagt cggcttgggt ggaggagatg cctcttcggc acggaccgcg
    56821 atcgatgaag tcaacagggt gacgacgcag caggcgacga cgaagaggca accacgagga
    56881 ttcggcatgc ggctatgttc tttctatgcg agaaggagga gtaccggcat ggggcgacca
    56941 gagcacatcg gccagcgccg tcaccacctg ggtcaccctc gatacggtta ggttccctgg
    57001 aaacaggttt tgagaaatgc cacggtggcg tcccaggcct gcgcagtggc ttcagcattg
    57061 tgctccgctg gtctggtttc gtcgcagaag gcgtgcgtgg tgcccggata ggtcttgatc
    57121 tcgatgcgtt tcccctgggc cgcagcagac cggagcagat cggcgtcggc ttgtgtcgcc
    57181 cacgtatcgt gttccgcctg atgatacagc aggggactca tcatgttcgt gagttgatcc
    57241 ggcgccgtga ccttgccgta ataggccacc gcggcccgca accgtttgcg ctggcaggca
    57301 aaacggatgg ccaacgtggc gcccaggccg aatccgacca cgccgtggat gttccgcttc
    57361 gtgcgttcgg tggtattgag atattcgcag caggtgttga tgtcctggag caggagttgt
    57421 tcgttgcact tggccgccaa cgcttccgcg acttcggcat tggccgtcac catgccgccg
    57481 agccggccat agagtttcgg gatgatcaca ccgtatccct cacaagccaa ccgtgcgcca
    57541 agggccgtaa tctgagagtt catgccccac cagtcatgca ggagcacaat cccgggatac
    57601 gtgaccttgt cctgcggcca gaactgaatg cattccacat ggacgtcttt gctgacgcgc
    57661 gtgcggatgt agggatccac catcgcatcg gtcgcagtgg gaatcgccat tccactcgga
    57721 aagcgagccg tgccggttcc aatctgatcc agggaaaatg gtgcggtctg cgtagtcatg
    57781 ggtgatcggg tcctttctcg attggcgtct gcgtccagcg ggtagccgcc tggtactata
    57841 gggagcggat ttgcacattg tcaatcgacg gaatggtagc gggtgaggag tcttgacccg
    57901 tcagagtgcg gccggtacag tgcgcgccaa cgatgcctca cgaatctcgc atgccgctcg
    57961 gaatcggctt gatcgggctt ggccatcatg gcagccgtta cgccaaacat ctcttgcacg
    58021 accttcccga ggcccgtcta gtcgcggtgt gccggcgtcg ggccgagagg ggaaacgggt
    58081 tgcccgatgc ggcgaccgtg ccctgctatt ctgactatcg cgaactgatt gccgattccc
    58141 gggtcgaagc ggtggtagtg gtgacgccgc ctacactcaa tcgggcgatg tgtacgtctg
    58201 ttgcgcaggc gcagaaggcg ctattagtgg agaagccgct ggcgacgacg gcagaagatg
    58261 cgcgtgccat catgcaggcg gctcgggcga gcgggcaggt aatgatgacg gcacagactc
    58321 tgcggttcga tgccgcggtg agcgcgctgc gtgcgcggca ggcggaggcc ggtgcgctgc
    58381 agtatctcag cctggccagc cggatggaac cgcatgcgga cagtgaagac tggggattcg
    58441 gcgggcgagg ctgtgtcctg gagatcggaa tccatctgct ggacctggtg cgtgtgttga
    58501 cgcaagacga ggttctcacg gtgtgctgtg agatggatcg cgtggcgcct gccggtgccg
    58561 agcggcggat gctggggcgt ttgacgaccg gaagcggatt ggtctgcgtg ctggatgtgt
    58621 cgcgggtcac atcggggcgg atcgggcggg tggaattgat cgggagtgaa gggcagttat
    58681 cggcggactg gtgcgggcaa cgtgtaacac gggtctccgc gcgacacggg gcaggagaat
    58741 ggccgaccgc ggcggaacca accgtactca gcacactacg cgcttttgtg cgagctgtcc
    58801 gcgacggctt gccgcccccc atcaccattg aagacggcaa gcgcgccgtt gaaattgcag
    58861 aggcctgtta cctctcagcc agccaaggtg gccgggccgt cacggtcgaa taccggtgac
    58921 ctacgtctcc gcgacgatgt gcgtatcggt ttcttccacg ccttcgatgg catgaatccg
    58981 cgtaatcacc acgtcggaca gcgcccgctc atcggccaca ttgataaaga cgaaaatgtc
    59041 aggttggccg aaacaggcat ggatctgttc gactccggca atgcccttca gcgccgtgag
    59101 aacagacttc cctttaccgg ctttgacctt aataagaatg tacgctttcg ttgccatgat
    59161 gcctcctcaa gctgcaattc gtcacatagt gcggatgatg ttggctgcgc tcgttgctat
    59221 gaaatagtta ttccgatttg gccccgggca tttcccggcg acgctgggcg tagacttcct
    59281 ggaagctttt gccttgagta gaaaatgcgc gactgctctg ttggtacagt tccgcgagaa
    59341 tatcgaaatc ctgttttcca atattgaact cccggaaccc gtcactgaga ccatcggtgt
    59401 tccaggccgt ttcaagttgc ccggcggtta tcaccgcgtc gaaggtatcc gccgaccaac
    59461 cgctttggcg gaatgaggtt tccacctcgt cggcccgtgg acccagcttg gcgcgatgag
    59521 cttccgtaaa aaactgacgg atggctggat gatcgctctt gagaaaggcg gattggaatt
    59581 ggatcacggc tttgatgatc cgattggcct gcggggtgcc ttcaggcggc agaatcccgg
    59641 cgtcttcaaa cgtggccagc atggccatca ccgaaccgat gtagaccgat gccgtccgtg
    59701 acggttgggc aaccgttcgc atcaccggtg tcgtttcacc cgtcgcctgc tgactccaac
    59761 caatatcagg agccagcggc gcgagagaga ggagggtgat ggccagagag accctcatga
    59821 agaaatccgg cacctctccc ggccgtgctg ctggagttgc aggcatgaca ccgctcgtct
    59881 tggcgcgatc acggcaatcg aagagagacg gcatggcggc attatacaga agcgccgcta
    59941 cgcgcgctac cgcgggaaac caccgcctcc acgagctcgc cgcgacattg aattcagcgg
    60001 aggcaactgc ctataattca gccactggag ccactttgcg acctgttacc catcaatttg
    60061 ttgaccgact gatgacgttc cgggggaatc tgtgcttgcc aaggtcttga gtgcagcgat
    60121 tgtgggcatc gacgcacatc tggtggatgt ggaagtcgat atctccgccg gattgcctca
    60181 gttctccatc gtcggtcttc ccgatgcgac ggtacgtgag agtcgcgatc gggttcgtgc
    60241 tgccctgaag aatagcggct ttcaatttcc catcaaaaaa gtcacagtca atttggcgcc
    60301 ggcgaatatt aagaaagaag gcgccgggct tgatctggcc atcgccttgg gcattttggt
    60361 ggccgaagac gtcatcgctg cggaggccgt caacaacacg gtgttcgtcg gggaactctc
    60421 gctggatggg cgtctcaaac cgattccagg cgccctctcg atcggcgtcg tctgccgaca
    60481 tcgtcatcga atttttgtca ccgccgaaaa tgctgaggaa gcggccctgg ctgacggcgc
    60541 agaagtactt cctattcaca cgctgccaca agccgtggaa tgtttgcgtg gcgtgcaacc
    60601 catcgccgcg ttgttattga cgggcgatgg aggagccgca tcccttcttc aggaggacga
    60661 agactttgcc gatgtgaaag ggcaggcgca tgccaaacgc gcattggaag tggcagccgc
    60721 cggagggcac aatctcctga tgatggggcc tcccggatcc gggaaaacca tgttggcgcg
    60781 ccgccttccc ggaatcctgc ccgtgttagc gcaggaagaa gctttggaga caagtcgcat
    60841 ccacagtgtg gtgggacaac tttcacgaga tcagccgttg atgcgtcgcc gtccgtttcg
    60901 cgccccgcac cacagtattt cagaagcggg gctcatcggc ggtggaacca tgccgcggcc
    60961 cggcgaagtc tccctcgcgc acaatggggt cttatttctg gacgaagccg gcgagtttgg
    61021 tcgtgccacg ctggacgcgt tgcgacagcc tctggaagac ggacatgtca ccgtcacgcg
    61081 cgcgagcggc tcattaaaat ttcctgcccg ctttatgctg gtggccgcga tgaatccctg
    61141 tccctgcgga tattatggcg atcggaccag ggattgcgtc tgcagtgtcg cgcaggtgcg
    61201 gcggtatcgg ggacgattat cgggaccgct cctggaccgc ctggatctgc agatcgaggt
    61261 cccggctgtg ccgattcgtg ccctcggcga tgacgcgccg gtgaccgatt cctccgcgac
    61321 tatccgcacc agggtgatgg cggcacgcgc tcgtcaggcc gaacgctacc gtcgcgacgg
    61381 catttacacg aatgcgcaat tgaaaccgcg ccatctgaag cagtattgtg ctctggatag
    61441 ccaggggcgc gagctgcttg agcaggccat ggcgcgatta gggttttcgg cgcgcgcgca
    61501 tggacgtatt ctccgtgtcg cccggactat tgctgacttg gccgagtctg atagtatcgg
    61561 gccggcccat cttgccgagg ctattcaata tcgaagtttc gaccgtcgag tggagttgtg
    61621 agagaaaccc ccgtcattca taccgcgcgt gtcttcgcct ggtggttcat cgtcggcgcg
    61681 acgatggcct tggcggtcat cctcctgcaa gggggcatca gggaggcgat gcaagctcaa
    61741 gggtcggtct gggagctcaa actcgttgaa tttctcacga ccattttggg tggtggctta
    61801 ttgggcggat gtattgcgtt gattttggac cgtatcaaga aatcgtgatg aggcatctcg
    61861 gaatgccggg cagcataagg agtggtggcc atggatattg aagtcgggtc gaatctgtat
    61921 cgaaactcca acgggatcat cgatatcgag ggcgtgccgc aattcgaagt cgcgatcaag
    61981 gaaccggcgc gggcgctgct cgtcaatttc gccctgttcg atgaagtggg acggatgatg
    62041 gcgaaggtgg tggacagcaa tctgaccttc aacgagcgcc gtgcctacca gctcgccaag
    62101 agtccgacga gtgtctcgct caaacacgaa gaatcaggga cggtcgtctt tgccttggaa
    62161 ctgcgtgagg ggaatcgcgt cgtattcagc aaggggtcgt ttcatacgat caagggccat
    62221 cgtctcgacg tgtcccagac ggaatggcgg atcgacaaga agcacttcag cggcaaggag
    62281 acggacacca agggaggggc ggtcttttta ggatgaccgc cctccgggca tgcatcgtcg
    62341 ccctgggtga atcatgaact atggggcgac cgtcggaacg atacagatct gcccgtcctt
    62401 caaatccacc acggccgtat ctccgtcccg caattctccc ttgatcaact gtctggccat
    62461 cggcgtttca atttcctgct gaataagccg cttgagcggc cgcgcgccat acaccggatc
    62521 gtacccgcgt cggccaaggc tctgtagcgc ttccggcgtc accgtcagcg tgatgcgccg
    62581 ttccatcaaa cgcgctcgca accgttccaa ttgaatctcc acgatcttcg ccaagtgttc
    62641 gttgcccagg gcatggaaga cgacgatctc gtcgacccgg ttcagaaatt ccggcctgaa
    62701 gtgagcccgc aattccccgg tcacttgatt tttcatcgtc tcataggagg caccggcttg
    62761 ttgcgcttcc aggatgtgct gactcccgat attcgaggtc atgatcaaca ccgtgttttt
    62821 gaaatccacg gttcgcccct gcgaatcggt gagccggcca tcgtcgagca cctgcagcag
    62881 aatattgaag acgtcgtgat gcgctttttc gatctcatcg aagaggatca cggagaacgg
    62941 gtgccggcgc accgcttcag tcagttgccc gccttcctca tagcccacat agccgggagg
    63001 cgcgccgatc aacctggcaa ccgtgtgctt ctccatatat tccgacatgt cgatccggat
    63061 caggttcgat tcgtcatcga atagggtgac agagagggcg cgtgccaatt ccgtcttgcc
    63121 caccccggtc ggtccgagaa acaggaagga accgatgggc cggttggggt ctttgatgcc
    63181 ggagcgggct cgcacgaccg catcggccac ggctgtgacg gcctgttcct gtcccacgac
    63241 acgcttgtgg agcagttcgt ccagcttgag aagtttttcc atttctccct cgacgagacg
    63301 agtcactgga atcccggtcc aacgactgac gacggcggcg atatcttctt cgtccacctc
    63361 ctccttcagc agacgggttt cgttttgctt tttgccaagc gattgctgct cgagttcaag
    63421 ttcccgctcc agcctgggca attcgccgta ccgcagttcc gccactttgt tcaaatcgta
    63481 ggcgcgttcg gagcgttcga tgttctgttt cacctcttct atctgctggc gaatcttccg
    63541 cagcttgctc accgaatttt tttcggattc ccatcgagtc ttaagggcct gcaggtcgcg
    63601 attcttttcg ttcagttctt tttcaatcga ttggagacgc gccttgctgc ctgcatcggt
    63661 ttcttttttg agggcttccc gctcgatctc cagttgcaag actttccggg agacttcatc
    63721 aagttccgcg ggcaaactgt cgatttctgt acggagccgc gcggcggatt cgtcgacgag
    63781 atcgatcgcc ttgtccggca aaaaccgatc gccgatgtac cggtttgaca gcttggctgc
    63841 tgcgacgagg gcggcatctt tgattcgcac gccatgatgc acttcgtagc gttctttgag
    63901 cccgcgcaaa atggagatgg tatcctcgac cgttggctga tccacgagca cagtttggaa
    63961 ccgtcgctcc agggcggcgt ctttctcaat gtgtttgcga tattcatcca gcgtggtcgc
    64021 accgatgagg tgcatttcgc ctctggccag catcggcttg aggaggttcg ccgcatccat
    64081 cgccccttcg gctgcacctg caccgaccac ggtgtggagt tcgtcgatga agaggagcac
    64141 ctgcccctgc gccgactgga cttccttgag gacggccttc aagcgctctt cgaattctcc
    64201 acggaatttc gccccggcga cgagcgcgcc catatcaagc acgacgacac gcttctgttt
    64261 gagcccttcg ggcacatcgc ctttgacgat gcgttgcgcc aagccttcga cgatggctgt
    64321 tttaccgaca cccggctcac cgatcaggac gggattgttc ttggttcgtc gcgacagaat
    64381 ttgaatggta cgtcggattt cgtcatcacg gccgatgacc ggatcaagct tgccctggcc
    64441 cgcgagtttg gtgagatctc gcccatattt ctctaacgcc tggtaggtgc cttccgggtc
    64501 ctggcttgtc acccgttgat tgccacggac ctgctgcaac gccgtgagga gacggtctcg
    64561 tgtcagattg aggcggcgga atacgccgcc ttcctcgacc atggccagca caacgtgttc
    64621 aacgctgatg aactcgtcac gcaattcctt catctggtct tcggctttgg tcagcactgc
    64681 accgagccgc ggtgtgagat ggatttgtcc gggagcagca ccaggccctt gaacttgagg
    64741 cactttcgct agtgcctgat ccgccgcctg gcgcacggcg gccggcgaga caccagcctg
    64801 ttcgaacaga gagctcgcaa tccctcgctc ttgctccaga agcgccagca ggaggtgctc
    64861 gacatcgata ccctgatggc cacggcgcat ggcaagggat gatgcgcttt gcagggcctc
    64921 ttgtagcttg acagtcatcc ggttcatatc catgtggctg actcctgggt ggatgtgtct
    64981 atctactgag agaacataat catatcgctg ggcaagtcaa taccagccga tacccgctat
    65041 ttcttgcggt gctgtctggc gactgcgtgg aatcttgccg ggtgtgactt gacctctcag
    65101 gggaatccat cttattgtcc gcccgacgtg gggagctcgc tttccgctaa gagccgcttc
    65161 tctctgttcg agaacctgca ttgactgatc catgttgacc atattcaatt cgacgctcca
    65221 actctatcga gacgcgcttc acgcaacctg gcgctccctg gttcgcggat ggttcgtgat
    65281 tctcgccgtc atcgtgttca cgctgctgat gatgttggcc catcaaattg ctcgtcccct
    65341 gggtatgctc gggggatttc tgctgggtgc cgtgaatgcg ctgttgatcg gtgcgacgct
    65401 cagcctgatc gagcagtcca tcaagggcat gcgaactatc accatgcatg acgtactgga
    65461 aagtatgggg cattattttt gggacgtcat cagcgtcggg tttgtgctct ggattcccac
    65521 catgctcctg gacacgagtc tgcgcgcgaa ccccgacagt caatttctgg taatggcttg
    65581 tttattactg gtcttcattt tgctgaatcc cgctccggag gtgatctatc tcaccaggca
    65641 tgattctcca ctggatgtct tgaagcgcag ttatgatttc gtgttggata attggatcga
    65701 atggttcctg ccgatggccc tggttttggc gccactcgga ttgtccctct tctttaaact
    65761 gtcgagtcag ttggggcggg gagcggggtt agatttcatt cagttggtct tagtgccctt
    65821 taccattctc agcgcctggc ttaccgacct cgggatttcc gccaacctca ccggaatgat
    65881 ggcgctgctg ctgactccac cgctcatggt gggcatgctc ctgtttcgtg gccatctttt
    65941 ctcggcgctc tcttccacgt ctcgtcgaca acgcgcgtat caatcgcagt ttcacgactc
    66001 agctcgctag gaggtctttc cagccagacc aggacggcgt cctgtttctg cataatatgg
    66061 ataacttgtt gaggggatga gtgaagtggt gtatggctta acctgcactg ctgcttctgc
    66121 agggcctcgc atccatcacc agggagcgga cctggtcgtg cgcctctcga ttttctggcg
    66181 gttagttctg acgtatctgg tcattatcgc ggtgatgacc ggcgtcaatg tctatgcgtt
    66241 gttgcagatt cggacactgg ctggcctcaa tacggaagtc gggtcccacc accatccgga
    66301 gattgattcg gcgaagcgct tgctgacgtc gatctatgaa caggttcagg gcgagaagaa
    66361 atatgtggct gtgcctgccg ccacatttct tgagcatttc gatagtgaga gcaaagagtt
    66421 tcaacaagtc ttacaagggt tgctcgctca cgatgtctca gagcccgaga cgaggctgct
    66481 gaaggagatc gaggggcttc agcaagcgca tttgtcacta tttcaatcgc aattggctga
    66541 gtcgagcggc catccggagg cgagtgccgg ttacgacgcg cggcgcgaag cggtggttgg
    66601 gaaaatggcc gcagcgcttc agcaatacat cggccttcac gagcggcgcc ttgccgtggg
    66661 cgtcaacgaa tcgcacgcca gctcggtgca tgccgaagcc gtcacgcgca atttagtgct
    66721 ggtggccctg ctcttcggtc tcggcatggc cgcagtggcc agttacaaca ttcttcgtcc
    66781 gttgcgtcga ctgcaggcga ccattcagga gatggggcag gggaatttca aggcctcatg
    66841 ggatgggcag gcccctcgcg aattgcggga attgggtgaa acggtcaagt ggatgggcac
    66901 caagttgcaa caactggatg atatcaaaag cgaatttctc gctcatgtct cgcatgaatt
    66961 gcgtacccca atggcttcga ttcaggaggg cacccatctc ctcttggacg aaattcccgg
    67021 tccattgacc caggatcagc gaacaacgct gcgcatcatg tccgacagta gccggcggct
    67081 gatgtatctc atctcgacca tcctggatct atccaaaatg gaatccggca tgatggagta
    67141 tcggtttgtg ccgacggatc tccggcgggt cgcagatatt tcaatcaata aaatccgatt
    67201 gttggcagat gcaaaacatg tacagctggt aattgaggct ccggtggaac ggcactgggt
    67261 cagggcagac agtacgcgga tcgaacaagt actggacaat ctgctctcga atgcgttgaa
    67321 gtacagcccc gagggtgcgg ttgtcaggtt gcagatgact ccccggcgcg atgaaggcct
    67381 gctcttcgtc gatgtggcag acgcgggacc gggtataccg gcggaggaat tgccacatat
    67441 tttcgagcgg ttttatcagg gacgcacgaa gagtcggcag gtttcggttg ggagcggcct
    67501 cgggttagcc ctggcgaaaa aagtcgtaga ggctcacggc ggaaggattt gggttgaaag
    67561 tgaagtggga aaagggacga ccgtgcgatt cattttatcg ttaatgaaac cggggatgtt
    67621 ggcctagtgc aatggtgtcg ccccataggg atggttctgt gggtaggact tgtgtccgcc
    67681 tgtggcaccc tcccttcgcc cccagctcag acgccatact tctcaaacga tgggggagat
    67741 ggcaaaatgt tgcaagccat tttacgtaaa gattcctcgt tagtcacgaa atgcgctagt
    67801 aaaaatacct gtgatcatgt attcttcacc cgcgcattga cgtctctgta cgaaagtcga
    67861 gagtcggcca tccataactt cgagaaagtc attgccgtcg cccccaagag ccagttggct
    67921 gtgtccagcc gactctggtt acaattgcta caggccggcg aagtgccttc cgatcagtct
    67981 tggctgcgtt ccgtctggaa tggccctgcg attgctcaga cgcagggggt cctctctcaa
    68041 acaattgaac gcacggtgcg ggatcttctc gaccgcgaac ttaccatcca acaattacgt
    68101 gccttacagg acagcgaagc tcaatccctt gaggccatgc agcgcgagct tcaagatcag
    68161 gagcgcaaag cggagctgtt caattccagg cgcgagtccc atcgcatgtc ggttgaaccc
    68221 gcgacgcttc agagcttgca acggcaagtc agcgaccgcg acaagaaaat cgaagaactc
    68281 acgagccagc tcgaagcact caagcgcatc gatcaggaaa tgagaggcaa aacccgtccg
    68341 atcaagcctc cctcgagtgt cctcccatcg caacaatcgg agccgtccaa accgtaagga
    68401 ccgtcatgga acaagaacga attctcgttg tggatgatga tgaaggtctg ttgcatttgt
    68461 tgcggatgcg attatctgcg cttggatttt cggtgacccc ctgcacgaca ggccgcgatg
    68521 cgctcgttgc tgcgcgacag gaaacgttcg atatcgcaat cactgatttg cgtttgcgcg
    68581 cagaggatgg gttggttctc acggaagaac tactccaggt tcagcccgga cttcccgtca
    68641 tcattctgac cgcgcacggg agtatcccca acgccgtaga agcgatgcag cggggggcct
    68701 ttgggtatct gaccaagcct tttgatgaca aggaattgaa agcaacgatc gataaggccc
    68761 tgattcagtt gcggatgacg cgggagatcc agcggctgaa gtccctggtc aaggagcttt
    68821 atggccttga aaatgtcatc gcaagaagtc ccgccatgca gcgattgttt caacaggtcg
    68881 ctcaaatcgc cgattcagat gcaaccattc tgttgaccgg cgagactggg acagggaaag
    68941 aagtcctggc aagagtcctc catgcgaata gccgtcgagc caaaggcccg tttgtcgcat
    69001 taaactgcgc cgcgatcagc gaacatcttt ttgaaagcga actgttcggg catatccgtg
    69061 gagcgtttac gaatgccatg gcggcgaagc gtggtctttt tcaaagcgca aatggaggga
    69121 cgcttttcct cgacgaaatt gcggaaatgt ctctgcccat gcaggtgaaa ctgttgcgtg
    69181 ctgtgcagga gcgggaagtg cgtgaggtcg gtgcggatta cacgacaaaa gtggatgtgc
    69241 gcatcattac ggctacgaac aaggatttgg cggaatgtgt gaagcagggg acgttccgac
    69301 acgacttata ttaccgaatt tccgttgttc cactgaatat tcctgccttg cgggaacgaa
    69361 gggacgacat tcctctcttg gcccaacatt ttttgaagca gagtgcgagg cgttctaaca
    69421 aggatgttcg tggatttaca ccagcggcca tgcatcggct cattggatat ccatggcctg
    69481 ggaacgtcag ggagcttgaa aatgccgtgg aaaaagccgt ggtcatgtca cggcaggaca
    69541 tggtattgcc ggaactactt ccctcggctg gtgcgtcgac cgatattggg ttgaagccgc
    69601 tgaccgaagc aaaggaagaa tttgaacgtt cgtatttgcg caatgtgtta cagatgacgg
    69661 gcggaaatat ctcgcgagcg gcccagtttg ccggccgata tcgtgctgac ttttataaga
    69721 tgttaaagaa gtatgggctg catccttcaa tgttgaagga gcgccatgac ctggatctcg
    69781 ctgagattga ggagtctgct gaggaactca aagacgttta gcgttcaagt ggggggagcg
    69841 tgcagaggtg atttcctatt aagcgttcta atcgtcttgc cgttgcgtga cggatctttc
    69901 taatctgaat cccgaaatct ttcccgctca cccactgaac tgacgcctct tctatttcta
    69961 acggcgaatt ttgattgggt aaatccattc gtaaggtaag tttcatacct atgctgactt
    70021 gatgatcgcc ttgcagccgc catccgccac tcgaaatatc cctgattatc cccttcccga
    70081 gaaaatcgtc tgataagtag tagccaacat tgcactgcgt gaccctctgg taggtgcgca
    70141 gcctaaaatg ctttgacatg cagagctcct atgaagatca ggtcatgcac tcgttcgagt
    70201 agcactcact gctctgttgc tccgagtagg acgatgccgt tcctgcggat tgatggatgg
    70261 gcagcagcag gaactctccg gacgaattgc tcaagacggc gctgttcatc ttcagatgtt
    70321 cgaataaatt cgatgccaag cttagaacct tctacccatc tgactgcagc cagcggtaca
    70381 tgcagcggag cttggtgatc aggcaggaac aatcttagtt gcaagtactc tcccgcagtc
    70441 atttgtttcg gactttcaag gagacagcct ggaagagaaa tatctaaaat gcggccttcc
    70501 cccacacaat tatctccggc aaacatgacc gaacagtcca taggcactcg ctgactgtat
    70561 cgattctgca tggtgcctcc tcctcgtatg cctcgggacc tatactgtcg ctttcaatgc
    70621 gactgtaatc taagaaccag cgtacgccta gtcctcagaa ttggtaacac tatagtgggg
    70681 acgctgcgga cccttggcga caggaatgcc tgagggctaa aaactgcaga atttacgggg
    70741 ttcagggaat tcttgccagg ttggatcttt atcagtaagc gggacaaaca ccgttcgacg
    70801 ctgccagtcg aagccttcac ctgaaagcct ttgtaggagc aatgctaccc cacagcattg
    70861 actcagcttt cagacagaag aatctctttc tacgtggcat gtcagcgtgg gttagatgta
    70921 ttcaactggt ggtcggacga ccagaaaaat agttgccatt ggcaggagat ggatcgccta
    70981 tgcagcaaat atggtagcag gactgtagta actggctcct actttgattc cctcccttca
    71041 accctctgat agttctaggt atttg
//
LOCUS       DAOQDS010000012        70144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_121_length_70144_cov_19.869238, whole genome shotgun sequence.
ACCESSION   DAOQDS010000012 DAOQDS010000000
VERSION     DAOQDS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 70144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70144
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121_length_70144_cov_19.869238"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PK589_04395"
     CDS             <1..372
                     /locus_tag="PK589_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18866.1"
                     /translation="LPDAVTRQIVGTQVGHALEFEKNLQRPSSDGAEREPYGSATKGT
                     ILATSIGLPLISASPTSGQASALGASTSGSTIISPPTGGALQAAGVGGTGPAQSGGLN
                     SSKLLQQILNDVGKIKKGKDR"
     gene            complement(832..1116)
                     /locus_tag="PK589_04400"
     CDS             complement(832..1116)
                     /locus_tag="PK589_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18867.1"
                     /translation="MRMILAALCLCVSTALCTVSSYAAENSGGGKNGSNQGMTLDDIG
                     RGLKSAAKNIEEEIPKVGPAISDTFKKVRDGRKEDSSAKASPQHSSKDKK"
     gene            1409..2704
                     /locus_tag="PK589_04405"
     CDS             1409..2704
                     /locus_tag="PK589_04405"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNJ18868.1"
                     /translation="MQTTAFTSFDSLGLSPTLLRNLTKAGFSEPTAIQAQAIPHALSG
                     RDVLGCAQTGTGKTAAFVIPMLERLTGTPKGQPRALILAPTRELAIQIQATIDTLGRD
                     LQLFATTVVGGADMQAQVRGLRQRPDIIVATPGRLLDHMWNGTISLLAMNILVLDEAD
                     RMLDMGFAQQINQILDAMPEERQTLLFSATMPMDLARLAQASVKDPVRVMVAKSATTA
                     EGVAQAVHHTTHDRKNALLMSLLQSESDTVLVFARTKHRADRLGNLLDTAGHRVAVLH
                     GGRTLPQRRAALEGFRRGTYRVLVATDIAARGIDVANIAHVINYDVPNCPEDYVHRVG
                     RTARMRTTGRATTFVTAEDQEQLRAIERLLGQAVPRAEGSPASLPPSSRPDGHAPRNE
                     PERRRRRGGSAQGWRQTTDGGSREANGGIKNGSDLGPVS"
     gene            2740..3081
                     /locus_tag="PK589_04410"
     CDS             2740..3081
                     /locus_tag="PK589_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18869.1"
                     /translation="MSDQNQDAPRAATQWVRIPDGTMVQHRLDGQKGHIDGLTEIVTG
                     PSRNPDGRTQYRINVGKGDRVLIAEEDLLILTDAEGMVRMAKEKGEYRSLVSKQLRGI
                     FGEDRFIVKTS"
     gene            complement(3361..3642)
                     /locus_tag="PK589_04415"
     CDS             complement(3361..3642)
                     /locus_tag="PK589_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18870.1"
                     /translation="MKITSVWRGNKPNHKKKRTVPKLRIRWTELGLVDPSVEPHETPM
                     DQIRREVTALASSGFGAETRARSEQTQTRPARTLARTSTTRTARRPAGS"
     gene            complement(4003..5556)
                     /locus_tag="PK589_04420"
     CDS             complement(4003..5556)
                     /locus_tag="PK589_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ18871.1"
                     /translation="MQRATLNFETLLEVTNALNSQRDIESLWRVIADQIQKVIPWDRA
                     GITLYESNTDAFRFYAVITNMASPALAHDSVIPREGSAVGWVYDHRRLHIRGNLQKEQ
                     VFLEDRFYAQEGLGRMINLPLLVREHCLGTLNIGSVHGGEPDPDDCKFLQQVATQIAY
                     AIDHVLAYQQIKQLSERLRRENEYLAEEVKASRNQRLLVGASSSFSKVVELVKAVAPT
                     DTTVLLLGETGTGKEVLAQALHDLSARSQKPFIRVNCAALPSGLIESELFGHERGAFT
                     GAQLRRAGRFELAHTGTLFLDEIGEMPLETQAKLLRVLQDGMVDRIGGTQPVSVNVRL
                     IAATNADLSAAIQQGTFRADLYYRLHIFPIAVPPLRERREDIQLLAQHFLTQIGAKHK
                     RPHLTFDPQSMARLLSYNWPGNVRELQNVIERAVILSSSSQVTVDEMLLPIVRAGRAV
                     LTHQPESLEDLERHHIIMILERTKWRIYGDQGAANLLGLNPETLRSRLRKLGIRRPVS
                     RSSETSPMA"
     gene            complement(5563..7248)
                     /locus_tag="PK589_04425"
     CDS             complement(5563..7248)
                     /locus_tag="PK589_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNJ18872.1"
                     /translation="MWKFLLLWFAFTLPSGAILAPTSLMAEAGHVGYGPTSPALFSSA
                     ESLVIDTDPVALLPPPAAFDIRNFWMRADLRVRPEWRSGVCFGGGPPMNGACNSLNLS
                     GSGTSAHDGSKASDFFIQQWARLGLGYDPSPTLNFYVELQDSATWGGSGNPTDGGQSA
                     DAANHQCGTQRPGQCRLGIRAGYALIRNLAGVEGLSAKFGRQYVVFGNQRLFGHFDWA
                     NTGYSHDGVMLSYARTDFDLKLGWFRHSETDLGQGHPGGSLTPNLLTCNQDVTSQSVC
                     NPAVAQHAADAGSDVDMVVFYNQIRGVPRMIVEPYYVLYYNRLPERANPGQYLPKSAS
                     QLRHMVGLRLELRDGNWDLVHETAYQFGRTADGFGFDNQRNLSINAWASGTWLGHTWY
                     AHPWKPRVAIGFDYASGDGDSNCVTPGGTLARSCGGNANTFENFFPTNFLHVGYMLNG
                     AWRNTVQPQVNLQARPTTRDHVEVWAMRKYLASARDNWYRGSQGPLIFSRSDNQTTHV
                     GDELDVAWSHMFADGTVSVAVIYGHFFSGPYIRHQLGTSMDQDWGIVQLWTNF"
     gene            complement(7381..7872)
                     /locus_tag="PK589_04430"
     CDS             complement(7381..7872)
                     /locus_tag="PK589_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18873.1"
                     /translation="MKNTIALYSTAFLIGAWPLLDLSQAQAFSNNPEPVVWNSNQKSA
                     AWAEELLGQVVTYQTMAEKNLIPGSFQPYVEQTSKIRELHRSGNRRATYDGVNALMVM
                     LEARVGGIDEHSAEALWDFCYRVTPDEFHARDRHIRAKGADTVKEHEEFMRNMEERAG
                     MSF"
     gene            complement(8156..10099)
                     /gene="glgB"
                     /locus_tag="PK589_04435"
     CDS             complement(8156..10099)
                     /gene="glgB"
                     /locus_tag="PK589_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNJ18874.1"
                     /translation="MSPQNLAQNPGPSATTRLTDDDVYLFNEGTQFQLYDKLGAHPMV
                     SHGEAGTYFAVWAPNAEQVSVIGSFNDWNRDAHILHPRGDSGLWEGFITECAKGALYK
                     YHVHSRHADYRVDKSDPLSFFNEIPPKSASIVWDLDYTWTDDDWLRTRGRANALNAPM
                     AIYEMHVGSWKRIAAEQNRSLSYRELAVPLAEYLQKTGFTHVEFMPLTDHPFFGSWGY
                     QTTGYFAPTGNYGTPQDLMYLIDTLHDHGIGVLLDWVPSHFPTDEQGLGFFDGTHLYE
                     HADPRQGFQLDWNTFIFNFGRNEVRSFLISSALFWLDKYHFDGLRLDAVASMLYLDYS
                     RKEGEWIPNRYGGRENLEAIAFLKRLNEEVYRHFPDVQTIAEESTAWPLVSRPTYLGG
                     LGFGLKWDMGWMHDTLTYMQKDPIDRKYHHHNLTFRMLYAFHENFVLPLSHDEVVYGK
                     GSLLGKMPGDEWQKFSNLRLLFGHMYSQAAKKLVFMGGEFGQPAEWSHDGQLEWSALE
                     LPLHQGVHRWVTDLNHTYRRESSLHEGDVDPSGIEWIDCRDAESSVISLIRKGRATDE
                     IILVVCNFTPVPRPNYRLGAPRGGYWKELLNSDAAIYGGHGWGNAGGIEATPVPLHGR
                     THSITCTLPALSVLFLKHSSSPL"
     gene            complement(10096..>11331)
                     /locus_tag="PK589_04440"
     CDS             complement(10096..>11331)
                     /locus_tag="PK589_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HNJ18875.1"
                     /translation="AHAASSSVLKGEQSNTSVKFGDCAIMKLYRRIEPGMNPELEVGR
                     ALTARHFTHSPSLIGALEYVQEAGEPMTLALAQTFVANRGNAWDYTLEQLSRYINGIL
                     ALDHRKEEAGQPAHDRSNQEPTTSDLLLGYRDSAHLLGQRTGELHIALAQPSDEAAFT
                     PEPCATSYVQSRVHAMQQSATQALALLRRRLPSLSVTDQEQAQKVLEQESPLLSRLAT
                     LARQPLSALRIRCHGDYHLGQVLYTGEDFVIIDFEGEPAKPLAERRAKCLPMVDLAGM
                     IRSFHYAAHVALRTGEARRPHLPASLDLVSWMEHWYRTVRTAFLRGYRTTAGQAPFSP
                     QSPDEFNLLLDVQMLDKAMYELTYELNNRPDWVGIPLTGLLHSMSASIAIEDADNDDV
                     RNDGAIPRSGGQVRDEVNQ"
     assembly_gap    11332..11431
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11432..11966)
                     /locus_tag="PK589_04445"
     CDS             complement(<11432..11966)
                     /locus_tag="PK589_04445"
                     /inference="COORDINATES: protein motif:HMM:NF037041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18876.1"
                     /translation="MITVRDNWKVAFEGSSRAEIEKLLPDFLTASRWFGGKAKTIRSA
                     KLADVLQQEHGDGTMVLALIDVSYLEGGADRYTLPMTAAFDAEADRVKHDYPEAIIGA
                     LTVLQNGTALAGILYDALWHPDCAYSLLDSMGRRGQFRRSIGTLVGSATELFDEASHC
                     AHAASSSVLKGEQSNTSV"
     gene            complement(11991..14027)
                     /locus_tag="PK589_04450"
     CDS             complement(11991..14027)
                     /locus_tag="PK589_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3416 domain-containing protein"
                     /protein_id="HNJ18877.1"
                     /translation="MPSKPASRKKQASSPVLGEGRLRVAIEHVEPQADDGRYPIKRVV
                     GETIIVEADLFADGHDVIQGILRSRHERQEEWLTTPLSPLVNDRWRASFDVTELGQYR
                     YGIMGWVDPFATWQRDMRKRLRARQDVTVEILIGKHLIEQATAPAPDVSRRRLKDIQA
                     RVSRSDLASQALLDLLLEEDTAEIMADCIDHALMSTYDRELIVVVDRPKARFSAWYEM
                     FPRSTTDEEQPHGTFKDCESRLPYIAEMGFDVLYLPPIHPIGETHRKGRNNNPRGDSS
                     SVGSPWAIGSRDGGHTAIHSELGSLGDFEQLVAAAQTNGIEIALDLAFQCSPDHPYVK
                     EHPEWFKTRPDGTIQFAENPPKQYQDIFPLHFETDAYPDLWKELREVVQYWIDHGIRI
                     FRVDNPHTKPFLFWKWLISDVKASYPETIFLAEAFTRPKVMYHLAKIGFTQSYTYFAW
                     RNSKQELTDYFTELTTSSVREYFRPNLWTNTPDILTEHLQHGGRPVFMARLALAATLG
                     SSYGIYGPAFELYENRPQKPGSEEYLDSEKYEVRRWDINRPDSLKEFISLINRIRREN
                     PALHRDESLQFHPIDNEYLLAYSKQSADGSNVVLIVVNLSPHHVHSGWMELNLAALGI
                     EANQPFQVHDLLTQAYYVWQGPRNYVQLDPHSVPAQIFTVRRNLRREEDFDYYL"
     gene            complement(14060..14761)
                     /locus_tag="PK589_04455"
     CDS             complement(14060..14761)
                     /locus_tag="PK589_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18878.1"
                     /translation="MSDLWRETMVAGFRDTVMQIVLVLPRLLALLTFLSLGLLAAWSV
                     KVLTLRVLRTFGFDSFCERLGIVQPLSQAGVRRPVSLVVSGVLFWIVFLLFAFMGVDA
                     MNLPATANLISQIVAFLPNVLAASLVLLVGVLCANFFAEAALIAAVNAQVEEARVVAG
                     LVRWGLLLFTCAMVLTQLGIAKEIVIAAFSITFGGVVLALALAVGLGARHLMREILER
                     RFSQHEHKADQLSHL"
     gene            complement(14754..>15353)
                     /locus_tag="PK589_04460"
     CDS             complement(14754..>15353)
                     /locus_tag="PK589_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HNJ18879.1"
                     /translation="AALAGGYRVCHTCLGTRDQESTLGAVDLSVLIAQSVGGIFHSME
                     AYESSWEAIKASSEVPLYGEPESAPDPGPLHTGRMISGFKQGLRDLLPLWELILSRDT
                     VGQIIALDIQDPEEFRFPPSLWVQTVYDFSLAYHNQTLHREHMLKALTPLYLGRTASF
                     VLETQHGDAGEVERTVETVCREFEAAKPYLVEQWRWRDE"
     assembly_gap    15354..15453
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15454..15692)
                     /locus_tag="PK589_04465"
     CDS             complement(<15454..15692)
                     /locus_tag="PK589_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18880.1"
                     /translation="MTTVMSKPQGGKSETEVLAPSPELSVSRTDMEWPTDTIDIMIGL
                     LTLNNAGSIEPVLKSIVEGMRQFFPHASALLVNCDA"
     gene            complement(15709..16908)
                     /locus_tag="PK589_04470"
     CDS             complement(15709..16908)
                     /locus_tag="PK589_04470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ18881.1"
                     /translation="MHSALDEYREVSPKGTVDFLYRLSDLVQGKSFLHLNAVRYGGGM
                     SEVLRRLVPMMVSLGVEARWEVLAGSQEYFVVVTRMLNALQGRDDKLTDDMYQTYANV
                     IERNAKALKLEADMVMVHDHQPAGLVDYRTNGCWLWRCHLDLAQPQRPAWAFLRRYVL
                     KYDAAIFSLSGFAQRLPIPKFLIYPSIDPLSPKNRDMSRSEIAQVLDRLAIPRVKPTI
                     LQVARFDRFKDPLGAIHTYQLVKRHHDCQLVLAGSGVMHDPEGEAVLMELREAGARDP
                     DIHVIQLPPEADLEINALQRSATVVLQKSVKEGFGLTVSEAMWKGKPVVGSTAGGIAA
                     QIVDGVTGYVVHSVEGAAFRIRHLLNNPGLMNRMGAMAREHVRRNFLITRQLGDYLTL
                     LKLLPAA"
     gene            complement(16957..18636)
                     /gene="treS"
                     /locus_tag="PK589_04475"
     CDS             complement(16957..18636)
                     /gene="treS"
                     /locus_tag="PK589_04475"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HNJ18882.1"
                     /translation="MLNQDPLWYKDAVFYELHVRAFYDGNDDGVGDFPGLIQKLDYLE
                     WLGVDCLWLLPFYPSPLRDDGYDVADFRSIAASYGTTDDFRRLLEEAHRRGMRVIVDL
                     ILNHTSDQHAWFQAAKASPDAPTRNYYVWSETDQKYPRARIIFVDTEKSNWTWDHEAQ
                     AYFWHRFFSHQPDLNYDNPEVRRAMIQVMEYWLDQGLDGFRCDAVPYLFEREDTICEN
                     LPETHQYLKDIRSSIDQRYKGRVLLAEANQWPSDVRPYFGDGDEFHMAFHFPLMPRLF
                     MGVRSEDRQPIVDMFKHTPDIPSACQWCLFLRNHDELTLEMCTGEERDYMYYAYARDP
                     QMRRNIGIARRLAPLLDNDRRKVELLNSIVFTLPGSPIIYYGDEIGMGDNIHLGDRNG
                     VRTPMHWTADRNAGFSKADPSQLFLPVVADAVYGYQAVNIDAQKQATHSLLNWMRRMI
                     AIRKKHKVFGRGTLQFLSPANEKVLAYIRAYEQETLLLVHNLAGSSQAVELDLSRYKD
                     VVPEELLGESRFPTIHEHPYVLTLGPYSSYWLHLPAVRSPTTAYSMEWSAI"
     gene            complement(18640..19314)
                     /locus_tag="PK589_04480"
     CDS             complement(18640..19314)
                     /locus_tag="PK589_04480"
                     /inference="COORDINATES: protein motif:HMM:NF039842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HNJ18883.1"
                     /translation="MVLTADAPFAFIGCSELQESIAHQADDEKELAELIEEVPLDSIH
                     FHTHSYFLRHGLIEGTYPNDFAQWVVMQIGDHVLGERLAVLDPFDYPDLENLREELIS
                     IIDDHLSRSPIIPRVVFGQPFHFKRSRILEVPIGLEARTLQEFRDIVTDIDVSSIYYH
                     MFEAHFRLRRAENDFSAWLRTSLKLTNLADRIRSINPYQGSLERLRSKLISACDDFLT
                     SESAKS"
     gene            19969..20685
                     /locus_tag="PK589_04485"
     CDS             19969..20685
                     /locus_tag="PK589_04485"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNJ18884.1"
                     /translation="MLNPYLLFWSIGLLFFVAEWLRPARKITYRAVFWRDLLALGAYN
                     LSFLLVVQVTDRIPVPQYLPVALYNLPTAGKLLLFYIVEDFGLYWVHRLMHTRVVWPI
                     HRWHHSPTSLYWLAGIRTTIPHIALFNVTYVAALPLLHDASGWAFQVIMVEHIVRNNW
                     MHVNVTWKSSWLEWVFVTPRYHHIHHSSDPAHQVKNLGALLTIWDRLFGTYYNPDDVT
                     GELKFGLSERVNPIRLVIGV"
     gene            complement(20823..22136)
                     /locus_tag="PK589_04490"
     CDS             complement(20823..22136)
                     /locus_tag="PK589_04490"
                     /inference="COORDINATES: protein motif:HMM:NF023301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase domain-containing
                     protein"
                     /protein_id="HNJ18885.1"
                     /translation="MRIMTVSEILFSSRPHLLYTPAMRTRRMSLLLLSLAVPTLFSGC
                     ETTDKMLGAAERAVGSTTGRTVLDIVHGKDPADIARQRVEQYGRDPQALLRDLRAIQR
                     DFQALMAALTGEVGKKWGTKEVKLPEQKKYVKYTQNYRSRAIVDFDAGNILIETLDEK
                     DPRASLKNAVVTTLLTPNDPRSVDLFSDKEITLTGDKEPYLLGLVVNQAGKPVRTPTE
                     AEQFAESLLSKSTTRPVEQENGAKTAHLVNIPMVTNFSHKQAEKYRTVVAQFAERYQI
                     SPSLVFAIIRTESNFNPFAVSSAPAYGLMQLVPTSGGRDAYRKAKGEDKAPSRDYLFD
                     PDNNIELGTAYLNVLTYSQLDDVTDMVSREYCVISAYNTGAGNVFKTFAKDQRTALQQ
                     INGLQPSTLYDRLRSGLPYQETRDYLAKVVGFRKQFVVIGENGAQ"
     gene            22282..22884
                     /locus_tag="PK589_04495"
     CDS             22282..22884
                     /locus_tag="PK589_04495"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ18886.1"
                     /translation="MNTRASAAVWCWVIVLLMTLAPSPGRAAQTPTEAVQVTMKDLLY
                     LLTELKETSRSAQRQWEIEQVVRRAFNYEEMAERALGEGSEKTTIADRRQFTRLFVQV
                     LRDDLADKLRDYSLPQVAYLSEQGDGTGTQVLVAPAGREVDTKIEFSVVERSGRWLIN
                     DVSIDGASIVATYQTQFSRILREGSFSDLMEFLKQKAVVA"
     gene            complement(23051..>23437)
                     /locus_tag="PK589_04500"
     CDS             complement(23051..>23437)
                     /locus_tag="PK589_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNJ18887.1"
                     /translation="DGIVLTIQPAVVLLATISHVCGLPDATTLYERRTRRRLHCTESS
                     IDDDSPPSLSSTSPDTALTGREREIVYLIGQALSNKDIAERLCISSVTVRHHLTSIFG
                     KFDVNSRQKLLLRAYEYGLLKVHAHH"
     assembly_gap    23438..23537
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23555..23962)
                     /locus_tag="PK589_04505"
     CDS             complement(23555..23962)
                     /locus_tag="PK589_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18888.1"
                     /translation="MMLCPATHCGQMMETDVRAGYDPDSGLECLFCPRCGHRGMKART
                     GVQLLFTGQHEYVFSYGPSLSHLKIVLSTVAINLFRTQGMPPTQLAAHVADWALLMGQ
                     VCGTVRFSGDLILSSCYEYCRREATKSPAVSSL"
     gene            complement(24008..24325)
                     /locus_tag="PK589_04510"
     CDS             complement(24008..24325)
                     /locus_tag="PK589_04510"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNJ18889.1"
                     /translation="MPERNAQAPAGDEPIRNISRGATSRSIPKRKDRLQPSPQAETTD
                     TAEDIRTRIEKLAYARYQQRGQQDGYDYEDWLEAERIVQGESAPQSGEGSGRSGTHAL
                     HSA"
     gene            complement(24383..24811)
                     /locus_tag="PK589_04515"
     CDS             complement(24383..24811)
                     /locus_tag="PK589_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18890.1"
                     /translation="MSPTMHPVKQAGRSRVMIVDSAMQFGLKLADCLATRGYHAVLVR
                     DLESTLPQLGEIQPAAILFSHDSGGSHCDTPGMDALRALNALCPQAPVLALSEPVHPV
                     LSGESRSGDTLSAKSASPPLEHPVEAVLLTTLGIPCVRLQ"
     gene            complement(24808..25113)
                     /locus_tag="PK589_04520"
     CDS             complement(24808..25113)
                     /locus_tag="PK589_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18891.1"
                     /translation="MTTLAEIEGLITRELTRRGACTLEALAQQLQTCTWNQVFMAVDI
                     LSRRGTLVLRPLPEFQYTVSLAGSGSSTLRHLIPVTAGKPSLGVAAVDQIMNKEGQR"
     gene            complement(25358..26047)
                     /locus_tag="PK589_04525"
     CDS             complement(25358..26047)
                     /locus_tag="PK589_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ18892.1"
                     /translation="MTGPFLDQTQQMEPIVTKILVVDDHAVVRQGVKQILNEQFQGAV
                     IGEARNAEEMIDRIRKFTWDIVVLDVGMPGKSGLDALKELKQVCPKLPVLVLSAYPED
                     QLARRMLKAGASGYLTKDSAPNELVQALKKILGGGKFVSASMAELLVANLNEEAGRPQ
                     HELLSDREYQVMCLIAVGKSLKEIADDLCVGISTINTYRARILEKMQFKNNTELTHYA
                     LENRLVNRLVS"
     gene            complement(26071..28314)
                     /locus_tag="PK589_04530"
     CDS             complement(26071..28314)
                     /locus_tag="PK589_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ18893.1"
                     /translation="MTNRPIHVLLIEDNPGDIRLVRELFAQTGNGQFTLTHVGRLDAG
                     IRCLSESLIDVILLDLSLPDSQGIETLIRMHAAAHGIPIVLMTGLEDEELGLHLIQAG
                     AQDYLIKGQTSAPLLARALRYAVGRKRLEEELREQTRFLQSVLNSMADGVVMADETGT
                     FRVWNPAAEQILGSGPAKRTVGHWSEDFTIFRPDRVTPYPAEDIPLARAIRGESVTGS
                     TLFLKRRSQGNDLWLDVSARPLRDEMGLIKGGVVVFRDITEARRTDEALRDSENRFRA
                     IMDNSPALIFLKDLEGRYLQANRQFETVSHLANKDLVGKTDEEIFAPAQAAVFRENDR
                     KVLQADAPMQFEESALHDDGPHTSIVVKFPLRNAEGRCYALCGIATDITDKQRAEEIR
                     QQLAKDRLLLLESTGEGIYGLDRQGRCTFINSAASRMLGYSPDELLCQDMHERIHHSS
                     PDGGIYHRTRCHIHETLVDGRGRKVDDEVYWRKDRTSFPVEYSSFPVIEQGKVTGAVV
                     VFLDITDRKRAEQQLTASHAQLRKLTARLESVREEERILIAREIHDELGQALTGVKLE
                     LSLLQDQLPSVPPTVHSRLESISGLVDATIQSVRRIATDLRPIVLDQLGLIPAIEWQT
                     HEFQSRTGIECRLDIYLRSACLSQTASTAMFRIFQEILTNVVRHAKASVVKITLQEQG
                     GGLVLEVQDNGRGITETELADPQSLGLVGMRERALLLGGSITFAGNAGSGTIVRVRIP
                     LDQPAQE"
     gene            complement(28311..28775)
                     /locus_tag="PK589_04535"
     CDS             complement(28311..28775)
                     /locus_tag="PK589_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ18894.1"
                     /translation="MVVPELVKPIEILLVEDNPGDVRLTIEALKEAKVINHLTVVKDG
                     MEALAFLRRQGSYTAAPRPHLIVLDLNLPRKDGREVLADIKADDSLKRIPVVVLTTSQ
                     DEQDVLKSYNLHANCYITKPVDLDQFVRVVRSIEDFWLGIVVLPVSHQPGAV"
     gene            complement(28807..30594)
                     /locus_tag="PK589_04540"
     CDS             complement(28807..30594)
                     /locus_tag="PK589_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ18895.1"
                     /translation="MIALPTTTVQTTAFDSSSRWTERILPILLFLCFFIATALGLYAL
                     SFLRAMLVAERGDELAMNAATVAETVDRVLFERYGDIQLFANDGVIRDGSSEERTARL
                     QHYHQLYWYYSWLGITDADGTVIAATDMRPASGPPPLNRAAFDAVRRSGTIYLEPVVP
                     SAMPPQQKAVGFMAPIYGPRGEFRGVVASRVPIDNLRSIFEQEGRLRYGKDPYDWLLL
                     TRDGAIISEKQTSAESPKGSAILDIPSRSRAASDRNQSGFVEEVHHRQGHSVVTGYAS
                     TRGYRDFPGFDWIVLFRQTHDQVYAPVDRLLWTVGGIGLLVILPLTGFGILVSWKLGR
                     ERSELLKTRQDLEKSVAELGRSNADLQQFAYVASHDLQEPLRMVSSYTQLLAKRYRGQ
                     LDADADEFIAYAVDGATRMQKLIQDLLAYSRVSTGSQPFEPTPMGAVLSYAMDNLLSA
                     IKESQAVITHDRLPTVRGDAKQLAQLFQNLLSNAIKFHGDQPPRIHISAQRREDEWLF
                     SVRDEGIGIDPQFAGRIFVIFQRLHTRAEYPGTGIGLAICKKIVERHGGRMWVESETG
                     KGATFWFSIPDPPQQPSVTRSEGATTGVA"
     gene            30750..31166
                     /gene="arfB"
                     /locus_tag="PK589_04545"
     CDS             30750..31166
                     /gene="arfB"
                     /locus_tag="PK589_04545"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNJ18896.1"
                     /translation="MLSISTHVSIPDEEIELTAVRAQGAGGQHVNKVSSAIHLRFDVR
                     ASSLPPFYKERVLELRDHRVSRDGVIIIKAQDSRSQERNRALALERLRELIQQAAIPR
                     IPRRPTKPTKGSKKRRLEDKSKRGQLKSLRSRPHEQ"
     gene            complement(31238..31555)
                     /locus_tag="PK589_04550"
     CDS             complement(31238..31555)
                     /locus_tag="PK589_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNJ18897.1"
                     /translation="MGNKLYVGGLPYAATESQLSNLFSAHGTVESARVITDKFTGQSR
                     GFGFVEMSTAEEAKAAITALNGSDMDGRQLTVNEAKPQEPRSGGGGGGRFGGGDRGGR
                     NRF"
     gene            complement(31849..32718)
                     /locus_tag="PK589_04555"
     CDS             complement(31849..32718)
                     /locus_tag="PK589_04555"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNJ18898.1"
                     /translation="MQQVSPTPVPAHIVHRVHHKFWTYLLFWSIVAGSVICVPGYGIL
                     YGYSWLDWTMFAVLYLITGLGITVGYHRLLTHRSFECPDWVKACLLVAGGWALQNSGA
                     KWTADHLRHHAHCDDPADPYNAKRGFWYSHCGWLLNPVPCNDERFGSRVMQDPVAMWQ
                     HRNYAAIVVVGLALPFVIGFLHNGWQGGIGGFMLAGVGRTFAVLNSTFCINSVCHIWG
                     EQPYGTSDSSRNSWLVSLLTFGEGYHNYHHTYPSDYRNGPLWYNFDPSKWLIYTLSKL
                     GLASSLRTASPGN"
     gene            32928..33278
                     /locus_tag="PK589_04560"
     CDS             32928..33278
                     /locus_tag="PK589_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HNJ18899.1"
                     /translation="MPTGYPRKATSMVLWSCVVIGLSGLYAPVNAAPDPASTGQEQPA
                     ATSEEPGSSGKRPEGQGRGGRPGRKACADDVKKLCAGVKAGEGRIMQCLREHTQELSP
                     ACADMMQQRGKHRP"
     gene            33442..33822
                     /locus_tag="PK589_04565"
     CDS             33442..33822
                     /locus_tag="PK589_04565"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ18900.1"
                     /translation="MSDTTRQEQFSVDLRQHPRLRVPAPFACSLSRLGLAKWLGRGGE
                     GIGVVFDVSMRGAKVMSEAGIQRGDRVSVSLSLPNQVSPMTVEKATVRWEREQVYGLE
                     FVDLSPVAEMRLRKFISLATKPAA"
     gene            33997..34935
                     /gene="lpxL"
                     /locus_tag="PK589_04570"
     CDS             33997..34935
                     /gene="lpxL"
                     /locus_tag="PK589_04570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HNJ18901.1"
                     /translation="MSNPQANHKRRQPRSVYHPMYWPTWVGITLFRLLSWLPYHAQLA
                     FGRQCGRLLHGLLTKRRDIVRVNLALCFPEQSPEERNRVALHSFESMGMGVLEIAMAW
                     WAKDPRAHCECTIKGLEHIRDALHRGRGVLLCGAHLHAAELAGRFMALEQPVAIVYRP
                     QNDVVADMVANRCRSRYYSELIQHRDMRGILRALARNQVVWYAPDIDAGSKRSVFAPF
                     FGVPASSLTATSRLAKVSGAAVVPCFYYRRADGSGYDIEVGEALDHFPSGEMIEDATR
                     VNRLIEGAVRRVPEQYFWQHRRFKSRPPGEPPLYRR"
     gene            35082..35459
                     /locus_tag="PK589_04575"
     CDS             35082..35459
                     /locus_tag="PK589_04575"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ18902.1"
                     /translation="MDQDKQVSPVRIELRQHRRCPVAETCLLSFAPFAPTLSFNGDAE
                     GEGTVLNLSPGGCKISSEAGVKVGDAMSLIILLPGETAPTAVDLALVRWGKDLSFGVE
                     FVSMGTAEQDRLRQFLASAAVSE"
     gene            35506..35838
                     /locus_tag="PK589_04580"
     CDS             35506..35838
                     /locus_tag="PK589_04580"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ18903.1"
                     /translation="MEETRTLRKSRRVAADCRLEFVGEDEIEGEAEVLDLSSTGCMAK
                     SDQAMPPGLRMKVSLLLPDGHEWPVRIEEAVVRWARGGEFGVEFLVMRPPQLQRIQQF
                     VIKHRPLG"
     gene            36031..36810
                     /locus_tag="PK589_04585"
     CDS             36031..36810
                     /locus_tag="PK589_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ18904.1"
                     /translation="MFTWVGRKTIAGYAYLAALATLFAQAVFDLIIPPKQGRGETFRV
                     LVRQILFTGVDALPVTTVIALLLGIIIVTQAGTQLPRLGAGGLVGSIIVVTVIRELGP
                     LVTAFIVVGRSGSAIATELGNMSVTREVVALRLMGIPISRFVIMPRMVGMVLSMVCLT
                     LYFDVVAVFGGYLVADAQLTIPLDAFAESVIRALSTTDVLMTAIKGLGFGSAVAAVCC
                     YHGLAVRSSYTEVPQQTTRAMINSFVLCLLIDVVVTVPLYL"
     gene            36807..37538
                     /locus_tag="PK589_04590"
     CDS             36807..37538
                     /locus_tag="PK589_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ18905.1"
                     /translation="MSEAAIIFDKIRAPMLGGDLWLELDLKIMAGECVVCVGPSRAGK
                     SLMVELAAGLIAPESGRVLVLGQGWSEVSDDAPGSVRLKVGVVLQQPGLLSNMTLFNN
                     VLLPLRYHRSAMPDRHREQAVMGELERLGLASLRDRFPAELNQGEARRGAIARALILE
                     PEVLLLDDPVAGLDADMVLGLKSSIDERRAQRPLTVLAALRTCSPFVDAADRLIVLRD
                     GHVEADGTRDTVRDLAPEALRRYVE"
     gene            37571..38563
                     /locus_tag="PK589_04595"
     CDS             37571..38563
                     /locus_tag="PK589_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNJ18906.1"
                     /translation="MMHYSHRLSSGRLAQIVGTFVLIPAVILAVAGFWMSRTEHLFES
                     KYLLKASLSKSYGLEPGAPIVVSGIRIGRVKQVDLNDRGTVDLTLQLLARYQDMVKEN
                     SELHITKSGVVVGQTQVDIAMGTASSPVLSEGSIIRAVEPRDIGDLLNEVEPVLMAVK
                     QTLLRVETITQDMQGGLKAGGKALEQVALATQELPAVVASVQRTIASVEQTATALPAM
                     TGSIKRTLSMVDRVTADVQQTTGRLPAILDSAQTTLTSVKRLSDSVSEVSQELGPIIH
                     SAETTLADMSILVRGAKQTFPFNRFVQNAGPAPKESPPPSREGTQSLRGDQLRR"
     gene            38722..39519
                     /locus_tag="PK589_04600"
     CDS             38722..39519
                     /locus_tag="PK589_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ18907.1"
                     /translation="MRGDLTRAAEEYEAARRMAESLDDTQGLIVAINDVGRVALRRGD
                     VPRAVSSHERAVALAQQRGEPDLLIASLTDLATAEHQSGALQEAEHRYEQALDLAQQR
                     GDRSAEATIRNNLGLIRQTKGDVEQAAQLFRQALALNQALGNLESEASNHVNLGMLAE
                     ARHEYDAAEREYERALELDKTAEQRMEIAADLLRLGKIADRRGFPDRGQTYSERAYQS
                     YLAQDAFAEARSALSQAVACAKKLERAGDIARLERELTRLTAVHPAR"
     gene            complement(39548..41857)
                     /locus_tag="PK589_04605"
     CDS             complement(39548..41857)
                     /locus_tag="PK589_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNJ18908.1"
                     /translation="MQPKAPLPSGDATLKSGNAEQVKRISGQILAAADVDQILLDLRH
                     EILGCFDAEDLTLFVVDSEKKEIFSKIPHLDTVQEVRTPITEQSLAGFCAKYLRPVNV
                     GDAYSLVELSAIHPSLTHDATYDKSTGFKTKQVLAYPVVAENKYLMGVIQLLNKKSGG
                     RFTRKDEECVAEIAKSLGTAFYTLRKTSKKPPSKYDYLLTNGKISQHDLDVALAEAKK
                     GTTDIESLLIEKYKIPKAEIGKSLANFHKCPYIEYNERTIVDIELLKNLNVDYLKKNH
                     WMPLKRDRAAIEILTDDPGDLDRVQDIKRTFPGLNIRFAVSLRRDISLFLASTTGTPD
                     VTNKMNENVSDILGELVNESQLEAQEEASAAGLDENDNAIVRLANQIIADAFRQGTSD
                     IHIEPYGEKRDTLVRFRVDGDCFEYMKIPPSYRRAIVSRLKIMASLDIAERRKPQDGK
                     IKFKIGENKEIELRVATIPTAGYNEDVVMRILAASEPLPVDKMGFSERNLREIKSIAE
                     KPYGIILCVGPTGSGKTTTLHSVLGYINTPDIKIWTAEDPVEITQYGLRQVQVHAKIG
                     FTFAAAMRAFLRADPDVIMVGEMRDKETADTGIEASLTGHLVLSTLHTNSAVETITRL
                     LDMGCDSFSFADAMLGVLAQRLARRICKDCKEAYQPSKEEYEELRLGYGPDYWDALKV
                     PFDANFRLYRGKGCDACNRTGLKGRVALHELLLGTDKMKKLIQNKAKTDEMLHAGMED
                     GMTTLVQDGIQKVLQGHTTYKEVKAVAIK"
     gene            complement(42096..43802)
                     /locus_tag="PK589_04610"
     CDS             complement(42096..43802)
                     /locus_tag="PK589_04610"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNJ18909.1"
                     /translation="MRVSETLIPTLREDPGEAETVSHRLMLRAGLIRKVAAGIYTYLP
                     LGLRVLRKIERIVREEMNRAGAQEVLMPVASPAELWRETGRWDFYGKELLRFKDRHER
                     DFCLGPTHEEVITDLFRREVRSYRQMPLNFYQIQTKFRDEIRPRFGLMRGREFIMKDA
                     YSFDRDEAGARLNYQKMYDAYNRIFARCGLTFRPVEADTGLIGGSSSHEFMVLAETGE
                     ETIVYAEGGTYAANVERAEVLPPDVSPLPALRPLTAVSTPGRRTVEEVTKFLTIAPSQ
                     LVKTLLYTTGKETVAVLVRGDHEVNEIKLKRLLGATDIELVAPELIPKLTGAPVGFAG
                     PVGLKQIRILADWSVKAMANFVVGGNQVDTHFTDVNWERDFTVAQFSDLRNAQAGDLS
                     PRKDGVLKTAKGIEVGHVFMLGTKYSQAMKATFLDAQGQEQLAVMGCYGIGVSRVSAA
                     SVEQNHDAKGIKWPIPIAPFHVTLLPLSQSEPVLQLAESLYRAMEQAGIEVLWDDRDE
                     RAGVKFNDADLIGAPFHLVIGEKGLAQGQVELKLRHTGETKKIAPDQVIPTLTALLQA
                     AS"
     gene            complement(43809..45200)
                     /gene="rseP"
                     /locus_tag="PK589_04615"
     CDS             complement(43809..45200)
                     /gene="rseP"
                     /locus_tag="PK589_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNJ18910.1"
                     /translation="MGTTFAWSPDVVSMLTHWALPFLVVLGVLVAFHEAGHFLAARWV
                     GVKVLKFSLGFGPKIFGRQIGETEYLLSIVPLGGYVKLFGEDEHEPLTPEDKKRAFVH
                     QSLWGKTLIVAAGPIFNFILAYLIYTAYIGLGYTLPVPSFKDIIPEIEAVLPGSPADQ
                     AGLKPGDRVIRVNEKEISTNAELLKYIAQSNGKQLTLDLTRGQQIKTVLVTPNKTTVQ
                     DNGKATTLFQLGIEERAPVITAVIPGSRAQAAGLVAGDRVLRIDGHDIFTWSQMTALV
                     RENPNHALQFEIQRNGSAQSVSVTPMGEKATVDGKPAEIGKIGISAQNQTILQTNEPL
                     KAPLLGAQATWGWTELTVVGIYKIITGDISRKNIGGPLTIAKTAGDAAEQGTSSLVFL
                     MAMLSINLGVLNLLPIPILDGGHLLFFFIEAIRRKPLEDRQRELAQQVGLVLLVGIMI
                     FAFWNDIERLISP"
     gene            complement(45226..46386)
                     /locus_tag="PK589_04620"
     CDS             complement(45226..46386)
                     /locus_tag="PK589_04620"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNJ18911.1"
                     /translation="MKNIVILGSTGSIGTNTLDIVERFPQEFRVIGLTAGSNDEKLEA
                     QIRRFRPAFAALASEPAAAKLRQRCADLPVKILSGNEGVAQVAQSPDAELVISAIVGG
                     AGLLPTLAAIKAGKQIALANKEPMVMAGALMQAEAHKHQVRIFPIDSEHSAIFQSLEG
                     HRREDVKRIILTASGGPLWGFSREQLQDVSPERALQHPNWKMGSKITIDSATLMNKGL
                     EVIEARWLFDIPETQIEVLVHRESIIHSLVEYRDRSMIAQLGLPDMRTPISYAMRYPE
                     RMPLDLPSLDLTEVSTLTFFKPDHDRFPCLQLGYDALRIGGSMPATMNAANEVAVEAF
                     LQNGIRFLDIPDIIRSTMDAHQPRPIDGLDDALEADRWAREKAESLVHALTR"
     gene            complement(46419..47255)
                     /locus_tag="PK589_04625"
     CDS             complement(46419..47255)
                     /locus_tag="PK589_04625"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNJ18912.1"
                     /translation="MDKARPRSSSETVRRVMAAVVFLPIFYILVHDLGPIAFFGLVAL
                     SGMLAVAEFYRLHLAQAPWPWWSWAGVVATGLLLSSIQWPTIVADRTVLIATVVMALC
                     MPLLSGKALRDSLNDGMVLVMGVLYIGLLLGYLLLIRAMPDGALLIFFVVLVTWAADT
                     GAYIAGKSVGRHPLAPVVSPKKTYEGLAGGILLACLGAVAARAWFLPSISLVDCLVLG
                     VILTLAGLIGDLAESAMKRSTGFKDSGALIPGHGGMLDRLDSLLFTGPAFYYYVAIVT
                     TV"
     gene            complement(47308..48093)
                     /locus_tag="PK589_04630"
     CDS             complement(47308..48093)
                     /locus_tag="PK589_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNJ18913.1"
                     /translation="MSDPRSRDIEPAPDSDLLSQLDPDLLPKHLAVIMDGNGRWAELR
                     GLPRIAGHQEGIKSVRELISLSLDLGIKVLTIYAFSQENWNRPAQEITALMGLLEHYL
                     STERSSLVEKGVRFQTIGRVTALPPSALRWVRTTEQETAHLDKLILNVALSYGGRAEL
                     ADAAKELARSVQEGRLSLEQIDERAMQQALYTHDLPDPDLLIRTSGETRISNFLLWQL
                     AYTELYFTPTLWPDFRRRETLVALIEYQRRERRFGRVFSSVSS"
     gene            48286..49728
                     /gene="tldD"
                     /locus_tag="PK589_04635"
     CDS             48286..49728
                     /gene="tldD"
                     /locus_tag="PK589_04635"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HNJ18914.1"
                     /translation="MTNNETGALARFGVTDRDIEQALGRVKVSAVDYADLYFEYCQSE
                     SVSMEEGIVKRATKSVGQGVGVRATAGEKTGFAYSDELTAKDLNIAADTARYIANSVQ
                     GTAPVPVTHRPPLARNLYPHDRATIEVATRDRVTLLNAIDAEARRYDPRIKNVMAAYN
                     TEYKVMLVATSDGLMVGDIQPLSRLQVTCIAEENGNRQVGTFGGGGRIGLEYFQNDQR
                     YLHFAREAAREAILNLSAVDAPAGVMPVVLGGGWPGILLHEAIGHGLEADFNRKKTSA
                     FSTLMGKKVASEVCTIVDDGTLPFRRGSLNVDDEGTPTSRTVLIEKGILRGYITDKLN
                     ARLMGIPVTGNGRRESYQSVVLPRMTNTFMLAGESDPQDIIKSVKKGLYAVSFGGGQV
                     DITNGKFVFSASEAYLIEDGKITRPVKGATLIGSGPDILKQVSMVGHDLKLDEGIGTC
                     GKEGQSVPVGVGLPTIRIDEITVGGTKASS"
     gene            49725..51083
                     /locus_tag="PK589_04640"
     CDS             49725..51083
                     /locus_tag="PK589_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNJ18915.1"
                     /translation="MTRMIGEQDYTTVAQDLLTRASKQGATAADVMVADGETLSVQVR
                     MGAVDRLTKAREKRLGLRVFFGQRSASASTSDFSRDSLERFVQETCALAQAVVEDPVS
                     GLPEPGQFATELPELHIHDSTKLQTDEQIALALRAERAAFAADPRITNSEGGECDSSS
                     GRIILANSHGFLGHYANSSFSLSVSPIASDAAGMQRDYWYGVNRSFAKLESPEAIGQE
                     ATRRTVRKLGARKAATGRVSVIFDPEVAGSLLSHLCSALSGYALYKGASFLIGQLGQQ
                     IAPDFVTIYDDGRMPGGLGTRPFDGEGLPTRKQAVVERGRLTSYLLDTYSGKKLGLPS
                     TGNAARSIGESPSAGPTNFYMAPGTTRPEDILASVKQGLYVTDLIGFGINMVTGDYSR
                     GASGFWIENGELAYPVEEITIASNLKQMYSAIETIGSDLVFRGRIASPTVKIAEMMVA
                     GN"
     gene            51104..51658
                     /locus_tag="PK589_04645"
     CDS             51104..51658
                     /locus_tag="PK589_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNJ18916.1"
                     /translation="MQRLMASMLGALLTIPAIGMAAEFHVTSPTIKDQSTIGSEHVFN
                     GFGCTGQNVSPALQWEHAPKDTKSFAVTVYDPDAPTGSGWWHWLIFNLPPGTTGLSAG
                     AGNPDSAGAPAGSIQSMTDFGQPGFGGPCPPPGDKPHRYIFTVFALKVDQLPLKKDAS
                     GAMVGYYLNQNALGKASVTGLYGR"
     gene            51694..52149
                     /locus_tag="PK589_04650"
     CDS             51694..52149
                     /locus_tag="PK589_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18917.1"
                     /translation="MALDAEQGKIMLQLITSRYEDRHWRKKIEKTLSLPQSGVGDERQ
                     QQMFLYLKLNLKAYKSRRADPDDWILGGYATKEVIDRAKFQPHLVGQGITAEDVAFLG
                     VDPGPDVDEAWWEDMLVAWFGAPEDEEATEATEEESPSETAAESHPART"
     gene            complement(52209..52880)
                     /locus_tag="PK589_04655"
     CDS             complement(52209..52880)
                     /locus_tag="PK589_04655"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNJ18918.1"
                     /translation="MSSPHIALQELADSLHDCQRCPLAKLGRRQVVFGVGNPQASVMF
                     VGEAPGFHEDQKGEPFVGAAGQLLNELLQSVGLSRADIYIANVIKCRPPNNRDPEPPE
                     VDTCKPFLLKQIALIRPKLVCSLGNWATQTLLERKVGITRVRGQAFYMKDFVLFPLLH
                     PAAALHQSSMLQPLREDFQKLKEFLDRHNQPAPAKEQEVASAARTLEIERPSSPAQQM
                     DLFGS"
     gene            complement(52975..54273)
                     /locus_tag="PK589_04660"
     CDS             complement(52975..54273)
                     /locus_tag="PK589_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ18919.1"
                     /translation="MLILGGGIAGLTAARSLAAKGHAVTVIEQGAHLGGRMWHAPPPI
                     LLEAHGATWSLLKTLGQDAVTRPLRHTPLEFLQSTGARIQFLHLPLPSPLNTLLGTTL
                     FQGLSMRDRWHLLSFLERTWEQDPPLPNDLDSRVADEWLTSIGQSETARHGVWNSLAR
                     LLLGAALEDISAGLFMRTLRRCFLTGARATKLIVPPQGVDSFLLAPLTRELDRLGVRV
                     KLNATATQVRFSHNRVTEVELADRTRLTADWYLSALPPQRLTPLLPERVVTHYAYFQQ
                     LSRLSESPLVMVRLHLDQPVRQTQLILLEGKTFHWIIRHPDEERHEQTSVLWAQAVGE
                     PSLLPQSTEELVRLAADAMAAAFPTTSPPRILDFDVMRLASAMLASKPGTHQYRPIST
                     SPFTNFFVAGAWTDTGWPANLESAILSGHRCAEAISTRPS"
     gene            complement(54282..55157)
                     /gene="hpnD"
                     /locus_tag="PK589_04665"
     CDS             complement(54282..55157)
                     /gene="hpnD"
                     /locus_tag="PK589_04665"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HNJ18920.1"
                     /translation="MMTPTEAQTYCTTLTKKSGSNFYYSFLFLPKARRDAMYTVYAFC
                     KEVDNAVDEPPPGSHPQEELARWRRELSAAYEGTPTLPVTISLARHVRELSIPQAYFE
                     ELIKGVEMDLTTTRYATFEQLSLYCYRVASVVGLICLHVFGTTSPRAQDYAVNLGMAF
                     QLTNILRDLGNDAECGRVYLPQEDFTRFGYREDDLLHRRYKPEFTELMIYEVGRAKEF
                     YAKAARALESLPREERQALTVAEIMRGVYSRILQRIEQSGYRVLGDRVTLTPSHRLAV
                     AAGVWLRSRLLSSAP"
     gene            complement(55154..56095)
                     /locus_tag="PK589_04670"
     CDS             complement(55154..56095)
                     /locus_tag="PK589_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNJ18921.1"
                     /translation="MRICSLVPGATEVVAALGLADQLVGISHECDFPPQLSHAPVMVR
                     ARIESRSLASEQIDAQVGTMLSAGEGLYELDERGLLAAKPEVLITQNLCDVCAITPTQ
                     LTGILRKLAPPPRLVTLNPRRLDDILLDIATLGNALGQAETATRFLSQLRGRLDAVHA
                     RIEKESLRPRVACLEWLSPLYTAGHWVPDMVAAAGGIDALAEAGAASHKIDWDVFAAS
                     EPDVIVLMPCGFTVARTKMELASLTSRPEWKDLPAVRRGQVYLVDALSYFSRPGPRLI
                     DGVEQLAAIFHPACFNHGLPEAVEQLTSDDIPTSMTR"
     gene            complement(56092..56799)
                     /locus_tag="PK589_04675"
     CDS             complement(56092..56799)
                     /locus_tag="PK589_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNJ18922.1"
                     /translation="MSELRALIFDFDGVIADTEPLHFAALRQVLAGIDISLTEQEYYT
                     DYLGFDDRGCFLAALQSHHRQASPTLLGELMDRKAQAYLSAVREHLAIFPGVRELVHD
                     AAARYPLAIASGALRNEIELILEEAGLRKAFLHITSAEDVTRGKPAPEPFLHAMAALN
                     RQPSQPALTPDDCLVIEDSLPGIRAARAAGMKVLAVANTHTVQDLGEADAITHSLADT
                     RLSDLRSRLWGPAQGRL"
     gene            complement(56955..57722)
                     /locus_tag="PK589_04680"
     CDS             complement(56955..57722)
                     /locus_tag="PK589_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNJ18923.1"
                     /translation="MVSSTGIPAIGSVWTDWRLLFASQPEPDLEFTEDDLDEAVPPPA
                     PPMNAPKRSKKGPFLWILLLLLVGGIGYVAMDPDAAMQMIQPYLDGGTETSQPVAQRP
                     PAPAPKPAPPAVQAPAAPIPAPAPAPSMAPPAVVDSVPPPPAEAPTPMPAPAPAAVVP
                     APVPAMKPASAPVRVAGPLYAEGQRVMVIADPSRPKSPMPLFADSIGTKTSTTVPAST
                     TLTILDGEYQKNGWIYSVRTQDGKKGWISERSLRLKR"
     gene            58006..58419
                     /locus_tag="PK589_04685"
     CDS             58006..58419
                     /locus_tag="PK589_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNJ18924.1"
                     /translation="MRSYREELWFETKTRRAYLNITAQVEAAVRKSGVREGLVLVNAM
                     HITASVYINDDEAGLLQDFDTFLERLAPHDATYRHNNTGEDNGDAHIKRQLMGREVVV
                     AITDGKLDFGPWEQIFYGEFDGRRRKRVLVKVIGE"
     gene            58573..59586
                     /locus_tag="PK589_04690"
     CDS             58573..59586
                     /locus_tag="PK589_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18925.1"
                     /translation="MLSWSRPDPLTRDLIHLINARRHDHGDTDAAIDTLQAFIEQGRE
                     QDLLTVLAALDEEMAEWMFDLVAEASCAVIMDGPDEEKNAYALMAALELPLQANLGQP
                     LGLKIDPVATADLLHRHLHIPAGVVVRVEPRLLTDATLEGLTLQTLNEHLASIADSQR
                     TQAIASRLYPPVENTAFLFFQLIGMPDPGLPDSLEEPERRALLEGLVTQCSELALAPD
                     TLEVPVYASYALFDAQNAVRLHRLADETAAVWRDLDPMVRARTIVHLHTQCGNGVYMP
                     VWTDLFDPELPEDHGPVWTSPDVWVFTADLPIEWPGEMLTNRLLAEGCTRFENHIVEA
                     PEN"
     gene            59639..60247
                     /locus_tag="PK589_04695"
     CDS             59639..60247
                     /locus_tag="PK589_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HNJ18926.1"
                     /translation="MAGSSRIGAVVLVTLLSCLVLGGIWGVVLFVLPQSESPPEALPV
                     SVPTPPLPVPAATQPPDRPMIDDAARPGVANAEPPVVVPQPSVPPVAEAARMPIGREV
                     KCALEMDALCPEEEGDRRACLQRKAAQMSQPCRPMLRDKLARMKENWQQLRVACEADR
                     RQFCREVPSAGGELLLQCLESHAQEVSDQCFQFLPKRGRLLN"
     gene            complement(60248..60970)
                     /locus_tag="PK589_04700"
     CDS             complement(60248..60970)
                     /locus_tag="PK589_04700"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNJ18927.1"
                     /translation="MSQHSIPPLLLVRSPHGGTRECEIRRTPFTIGRKPDNDLCLEDP
                     AVSAHHARIVQVQGVLFLEDLSSTNGSFVNEHPIDRRQLQDADALRFGTHRVIFREKP
                     GLAAEPIIPADMLVADKTMVVPSQAPRESPAPARKVGIVEILSGRTGQSHYPLTRQVS
                     LIGAQDDAVITLTGWFAPKTAAMISRRGEAYVVSQTESGKRILVNGRPVQREHALRHG
                     DVVEVAGITMRFVLRDERSHAA"
     gene            complement(60992..61816)
                     /locus_tag="PK589_04705"
     CDS             complement(60992..61816)
                     /locus_tag="PK589_04705"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNJ18928.1"
                     /translation="MHAWHVTHGAASDVGLVRRLNEDRYHADPAAGIFVVCDGMGGHR
                     AGEIASSRTVETIAHHLAEAETDRSLPLIGISRPEFSSATNRLASAVRLANHRVHQEA
                     ARRTEYAGMGTTVVAVRLTGAVLSIAHVGDSRLYLIRDGAVQLLTVDHSLVQEQMQSG
                     LLAAADMARAPHRHVLTRAVGVHPLVDVELGEMPVLPDDILLLCSDGLTAGVTADVIL
                     RVSQESIDPQAMADRLIALSNAAGGTDNATVIVALVTQRPTSLWDRIYTQLVSPPA"
     gene            complement(61825..63234)
                     /locus_tag="PK589_04710"
     CDS             complement(61825..63234)
                     /locus_tag="PK589_04710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNJ18929.1"
                     /translation="MTTSWGWIGPYFIVILLAVLVGPFLATLPLFTHTYLRPLGMNVA
                     QVVRFIADGICLLMIWSAASRARRDLKDNGKGQTFLRAILFPSASLLIVIAAFQAYEA
                     HGISVLGPPRQPLYTWLVTGGLIGSATWLTFAWVRHVDALTSMFAWRPRRRAVAEEPV
                     RAEKPDEVRAADEAIPAAPPRPTAGIAVRNGNGVPSTLGRYQVLKELGRGAMGVVYLG
                     KDPTIQRFVAIKTMRLDDIDKEEDLKEFRDRFFREAESTGRLSHPNIITVYDAGEQDG
                     LAYIAMEYLEGVLLNCYCQKSTLVPAKQALQIVATVAEALDYAHSQGVIHRDVKPPNI
                     MILKQRLVKVMDFGIAKMASASKTQSSMIVGTPRYMSPEQATGKDVDGRSDVFSLGIV
                     LFELLTGERPFDAENMPALVMRIAKSPHAPLMKYRRDLPTRVQSILDRALQKEIQNRY
                     RHAGDMAQDLRDVFQVMPR"
     gene            complement(63375..64223)
                     /locus_tag="PK589_04715"
     CDS             complement(63375..64223)
                     /locus_tag="PK589_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNJ18930.1"
                     /translation="MSRIDLHLHTTHSDGSCSTAEVMAFAKQAGLTALAITDHDIVDG
                     IPEATAIGKELGIEVVPGVEISSRLGESELHILGYFLNWTDPLLAQRLSTLRDSRHLR
                     NPKIVQRLNELGIPITYEEVRALAGTESVGRPHIARLLMEKKFVTSAKEAFDRYLANG
                     RPAFVDRELPEPAEAVRWIREAGGVPVLAHPTWVRTSAEGLRVLVRDLKAAGLGGMEV
                     HYSTHTPSQTTEYLDLARQCDLLVTGGSDFHGVTKPDIEVGIGRGQLKVSQKLLDPLR
                     KAATSS"
     gene            complement(64220..65158)
                     /gene="folB"
                     /locus_tag="PK589_04720"
     CDS             complement(64220..65158)
                     /gene="folB"
                     /locus_tag="PK589_04720"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNJ18931.1"
                     /translation="MAERIVIERLEFYGRCGVTEEERRKPQLIVVDLELDAAVEAAAV
                     SDRLSETIDYAQVAERIVALSTSLTCQLLETLAEQLVGMLFAEFPADRVRIWIRKVHA
                     PLAMVAGSVGIRFERTRAAHQSTHQALSAAPFLIQQLARLPKGHILDVAAGRGRNALY
                     LLAQGSQVEAIDRDTDALSALEAAAGTQRLSGLTTRVLNLEASADHPPSLGHECYDAI
                     VVFFYLHRPLFPVIIDALKPNGILLYETFTIDNYFRHQHPRRWEFCLAQNELLRLTSP
                     LRVLHYDEGEHDGGHGSGPCYTARLVAQKTGPETSP"
     gene            complement(65217..65687)
                     /locus_tag="PK589_04725"
     CDS             complement(65217..65687)
                     /locus_tag="PK589_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18932.1"
                     /translation="MFLPARVFTSKNRRTSLQSIPRRFNGQSIDEQIRAVQSAWVDVL
                     TLPLCLTVLAFYEWWRWLFSIPSNPLLLTVVAAVAIHATRRRRKAYKAELKQLHLNRT
                     PQPTVSDVTEFLYRGARRMVQDFTAKCSPATIVASARRFASPANWLTKRLFARS"
     assembly_gap    65806..65905
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(65937..66455)
                     /locus_tag="PK589_04730"
     CDS             complement(65937..66455)
                     /locus_tag="PK589_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18933.1"
                     /translation="MTMRHFVHIAAIIGVLAVSGSTEAAELAGAWKGVLSSPDGSRAE
                     VQIDFSPQGLPLYSYTNNKGVTRQVELSHVGQTIEYVPPGGGVQRMAVKRFEQGPGRV
                     SISIIGSFERASQGYMDQRQEAALFEYTLVPEGMKMRVTTRTASHFGDKDLIVGGDPS
                     SVVAEGVLHRLH"
     gene            complement(66500..67411)
                     /locus_tag="PK589_04735"
     CDS             complement(66500..67411)
                     /locus_tag="PK589_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNJ18934.1"
                     /translation="MAKTTTASAAKPKALSLHIGLNAVSPAAYGGWSGELAACEFDAK
                     DMAAIAKSQGMKSTVLLTKQGTRAKMLAAMRKAAKQLKQGDLFFLTYSGHGGQVPDVT
                     GEEIDKQDETWCLYDGQLIDDELYFELSRFAAGVRILVLSDSCHSGTVTRAVPPPIDR
                     TKVTGRSKMMPPAVAIRTYREHQAFYNKLQHDVAKAAGKAGTTDPDSVLAQLAVSPRL
                     TAIAKEFHPAVILISGCQDNQTSMDGDHNGAFTEQVLKAWNHGNYRGNYAKFHAAVKA
                     GLPASQSPNLFTLGAAGRFLRQQPFKV"
     gene            complement(67590..67745)
                     /locus_tag="PK589_04740"
     CDS             complement(67590..67745)
                     /locus_tag="PK589_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18935.1"
                     /translation="MVVKQGYRLIVVDKDGVLVSEFQLTAQALAAPETFVAAIKESIE
                     EIEEEDT"
     gene            67935..68957
                     /locus_tag="PK589_04745"
     CDS             67935..68957
                     /locus_tag="PK589_04745"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HNJ18936.1"
                     /translation="MHTTKLIAAFLVLVLVGVPTISFAKARGGSGGGFSSGSRSGSSG
                     SMGSRGSRTYQDNGAKPIEQSTTARPSATPPPSMAGNPSTASRPAPAAQPSWFQRNPL
                     LAGIVGGLAGTWLGHMIFGATDTSARTTEGGEGEQAAAGAGSSGMLLLLMVMGGGALW
                     WYMRSRRTPAPNFSGLSRTSVASGSLLAESSRAGFGAAAVEEEMTSTDKAAFQQALID
                     IQTAWSHQDLAGLRRLVTPEMLDYFSTGLAEQASEGVANRVEDVVLGQAEVRESWSEG
                     ATQYATVSLCWRAKDYTISLAKQPGEQGYIVEGNAELPIETTEVWTFMRFGAGKWLLS
                     AIQQVS"
     gene            complement(69159..69470)
                     /locus_tag="PK589_04750"
     CDS             complement(69159..69470)
                     /locus_tag="PK589_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18937.1"
                     /translation="MPISRRPELEKDNPVAPQIYVGPRRKRSLARKTSSELQAPSDEM
                     ATRLQRLVEAAEQFVHSMPPVQETDQERHALLEAISQAQGCLPIEDTAATRLRQASHT
                     R"
BASE COUNT        14110 a        19455 c        21222 g        14957 t
ORIGIN      
        1 cttcctgatg ctgtcaccag gcagatcgtc ggaacacaag tcgggcatgc cctcgaattt
       61 gagaagaacc tccagaggcc ttcatcggat ggagcggaaa gggagcctta cggttctgct
      121 acgaaaggaa cgatcctcgc cactagtatc ggactgccgc tcatttctgc cagcccaaca
      181 tcaggtcaag cttctgcgct tggagcatca accagtgggt cgacaatcat ctctccgccg
      241 acagggggcg cgcttcaagc cgccggtgtt ggaggaacgg ggccggctca atccggaggc
      301 ttaaacagta gtaaattgct gcaacaaatt ttaaatgacg tgggaaagat caaaaaagga
      361 aaggacagat aatcggctag tcacgcggcg cgtctgagct attgcgggaa cgaaaggcag
      421 gcgcccgaac gaactctcat caccgcctcc tgctcgaatc gctgcttgta aatgcttatg
      481 aggcgttgga tggcggtgtt tttcaccgca ttcccctcat gaattagttg tagaatgtgc
      541 gaatcctcgt gctcgatgga ggcattctgc tttcgccact gacccgaagc ctgccatgag
      601 gtaaggcctt ctggaaatgc cggtgtgacc gtgtcattga cgaaggccgc ccactcatca
      661 gggctgacca ctccgcgagg tgtcgctgtc ccgaagtaga ttgactccat tatggcgtga
      721 cgctcgccgg gaagacagac cggtgattga gtagcgaggc agccggcggt ggtgtgcagc
      781 acaaccaaga agcatgcgac aggatgaatg ggtctcattt tggagggagc gctatttctt
      841 gtctttggac gagtgctgag gggatgcttt ggctgaggaa tcttctttcc tcccgtccct
      901 tacctttttg aaggtgtcac tgatcgcagg gccgaccttc ggaatttctt cctcgatatt
      961 tttggctgca cttttcagtc cgcgcccgat atcatcgagg gtcatccctt gattagatcc
     1021 gttctttccg ccgccggagt tctcggccgc ataggatgat acggtacaca gcgccgtgct
     1081 cacgcacaaa cacagtgcag ccaggatcat ccgcattacg tgccctccta ttcgtgatac
     1141 tagtataaag cattctgcta ccacagcaag ccagggcaag cgtcttggcc gcctgaaaca
     1201 gcctctcctg ttgaccaatt ggggcaccgt gcggtaaagt gtgttctact gcagcattgt
     1261 atcggtccac caatgctgta cctcctcaac gtggaccgcg cgatcgcatc gcgaccaggt
     1321 ccagtttgga gctgactccc tccggattca ccctcccctc ggttctctga tcccgtctta
     1381 atgcgatcca caacgaaagg atagctgcgt gcagacgact gcgtttacga gttttgactc
     1441 ccttggtctg tctcccaccc tgttgcgaaa tctcacgaaa gctggttttt ctgaaccgac
     1501 ggccattcag gctcaggcca taccgcatgc cttgtcaggg cgtgacgtgc tgggctgcgc
     1561 acagacagga acgggaaaaa ccgcggcatt tgtcattccg atgctggagc ggttgaccgg
     1621 cacaccgaag ggacagccac gtgccctcat tctggcgccc acgcgcgaac tggctattca
     1681 aattcaggcc accatcgata cattaggccg agacttgcag ttgttcgcca ccacggtggt
     1741 tggcggagcg gacatgcagg ctcaggtgag agggttgcgg caacgtccgg atattatcgt
     1801 ggctactccg gggcgattgc tcgatcatat gtggaatgga acgatcagct tattggcgat
     1861 gaacattctt gtgctcgacg aggccgatcg gatgctggat atgggatttg cccagcagat
     1921 caatcagatt ctggatgcca tgccggaaga gcggcagaca ttgctgtttt ctgcgacgat
     1981 gcctatggat ctggccaggc tggcgcaggc cagcgtaaag gatccggtgc gcgtcatggt
     2041 ggcgaagtca gcaacgacgg ccgagggcgt cgcccaggct gtgcatcaca caacgcatga
     2101 tcggaaaaat gcgctcctga tgtccctcct gcagtccgaa tccgataccg tgctggtatt
     2161 cgccagaacg aagcatcggg ccgaccgact cggcaatctg ttggatacgg ccggtcaccg
     2221 ggtggcggtg ctccacggag ggcgtacgct gccgcaacgg cgagccgccc tggaaggatt
     2281 ccggcgaggg acgtatcgag tattagtcgc gacggatatt gccgcccgag gcattgatgt
     2341 ggcaaatatt gcgcacgtca tcaattacga cgtgcctaat tgcccggaag attatgtgca
     2401 tcgtgtcggg cgtacggcgc ggatgaggac caccggccgt gcgacgacgt ttgtgacggc
     2461 ggaagaccag gaacaactgc gagccatcga gcgattgctg ggccaagcgg tgcctcgcgc
     2521 cgagggaagc ccggcttcac tgccgccttc gtcacgaccg gatggtcacg cgccacgcaa
     2581 tgaaccggag cggcgccgac gccgtggcgg gtctgcccag ggatggcgtc agacaactga
     2641 tggcggatct cgagaggcca atgggggaat caaaaacgga agcgaccttg ggccggtctc
     2701 ttaatttgga cgagtctacg ttgcaaggga ggaagaaagg tgagtgatca gaaccaagat
     2761 gcaccgcgag ctgcgaccca gtgggtcaga attccggatg ggacgatggt gcagcatcgt
     2821 ttggacgggc aaaagggaca tatcgacgga ttgacggaaa ttgtcaccgg tccgagccgg
     2881 aatcccgatg gacggacaca gtaccggatt aacgtcggca agggagatcg agtgctgatt
     2941 gcggaagaag atcttctgat tctgacggat gccgaaggaa tggtccgaat ggcgaaagag
     3001 aagggcgagt atcgctcact ggtcagtaag cagttgcgcg ggatctttgg ggaagaccgc
     3061 tttatcgtca agacatctta acgacgatgc cccttcgcgg cctcccagga acctctgcta
     3121 gtacaggggt tcctggggcc cccatcgtaa cccttccaga tcgggatctt ctcctcccga
     3181 cgtcgcaggc cgttccggct tctcggcctt ccgttgcacc cgacgagctt ctttctcccg
     3241 ctgcttcacc tgcctggctt tttctcgatc gcgtttcgcg atggtcgttt tgcctgctcg
     3301 tgcgatggtt ccctccttct attcaccttc cccgatcggg gagcaaatag gcttgcctcg
     3361 ttatgatcca gctggccgac gggcggttcg ggttgtactg gtccgtgcca gagtgcgcgc
     3421 cggacgtgtc tgggtttgtt ctgaccgggc gcgggtctct gcaccaaatc cggaactggc
     3481 cagtgccgtc acttcacggc ggatctgatc catcggcgtc tcatgtggtt ccacggaggg
     3541 atcaaccagg ccgagttccg tccaccggat tctcaacttg ggaacggttc gcttcttctt
     3601 atggttgggt ttgttgccac gccagacgga cgttatcttc atcgtacact cctttcggcg
     3661 aacaacagga tggcgatgtc caggccatca aactgggtgc agtgggggag gaatactctc
     3721 gccccatgct ttgcccattt cctgtcagaa tgtaagaaat gggccgtagg aggagcgtaa
     3781 cttggggatg cgatcgcgcg gcaatgacgg aaacgcgaag cgtgggatct cgctcgttac
     3841 tcgtacggtt tagcaactta tcatgcttac gacaccttgg tcaaatgagc cgtccgcggt
     3901 caacaatacc cggcgattga ggcggatagc tcctcccgtt caaccatcga tcttatgctg
     3961 cttacgaagg agcacgagag tgatgcttga aatgtcaggg agttaggcca tggggctggt
     4021 ttccgatgat cgtgagacgg gccggcgaat gccaagtttt cgtaagcggc tgcgcagggt
     4081 ttctggattg agtcctaaca gattggctgc cccttggtcg ccatagattc gccatttggt
     4141 ccgttccagt atcatgatga tgtgatgccg ttcgagatct tcaaggctct caggctgatg
     4201 cgtcaagacg gcccgtcctg cccggacgat cggaagcaac atttcgtcca cggtgacttg
     4261 ggatgaactg gaaaggatca ccgcgcgttc aatcacattt tgcagctcgc gcacattgcc
     4321 aggccagttg taagagagga ggcgtgccat ggactgtggg tcaaaggtca gatgcggccg
     4381 cttgtgcttc gcgccgattt gcgtgagaaa atgttgggca aggagctgaa tatcctcgcg
     4441 gcgttcccgg aggggaggga ctgcgatggg aaagatgtgg agccgatagt acaagtcggc
     4501 tcggaaagtt ccctgttgaa tcgcggcgga caagtcggca ttggtggcgg cgatgagtcg
     4561 aacattcacg gaaaccggct gcgtgccgcc gattcgatcg accatgccgt cctggagcac
     4621 ccgcaatagt ttggcctggg tttccagcgg catttcaccg atctcatcca aaaacaatgt
     4681 tccggtgtga gccagttcga aacgtcctgc tcgtcggagt tgtgcgccgg taaaggcacc
     4741 gcgttcatga ccgaataatt cactttcgat cagtccggaa gggagcgctg cgcaattgac
     4801 gcgaataaat ggcttttggc tccgcgcgct caagtcatga agggcctggg ccagcacttc
     4861 tttccccgtt ccggtttcgc cgagcaggag cacggttgtg tctgtcggcg cgaccgcctt
     4921 cacaagctcg accaccttgc tgaatgatga agatgctcca acgagcaacc gctgattacg
     4981 gctggccttc acctcttcgg cgagatactc gttctcgcgg cgcaaccgct cgctcaattg
     5041 tttgatctgc tggtatgcca ggacgtgatc gatggcgtag gcgatctggg tggcgacttg
     5101 ctgcaaaaac ttgcagtcgt ccgggtcggg ctcgccacca tggacgctgc cgatgttgag
     5161 tgtcccgaga caatgctcac gcaccagcag cggcaggtta atcatgcggc ccaatccttc
     5221 ttgggcataa aaacggtctt ccagaaacac ttgttccttc tgaaggttgc cacgaatgtg
     5281 caggcgccgg tggtcataca cccatcccac cgcgctgcct tcacggggaa tgacgctatc
     5341 atgcgccagg gccggactgg ccatgttggt gatgacggcg taaaaccgga aggcgtcagt
     5401 attggattcg tacaaggtaa tccccgcacg atcccatgga atcactttct ggatttggtc
     5461 ggcgatgact cgccagaggc tctcgatgtc tcgctgcgag ttcagtgcat tggtgacttc
     5521 gagaagtgtt tcaaaattca gggttgcgcg ttgcatgacg aattaaaaat tggtccagag
     5581 ttgtacgatg ccccagtcct gatccattga ggtgccgagc tggtggcgga tatagggccc
     5641 agagaaaaaa tggccataga tgacggccac tgacacggta ccgtccgcaa acatgtgact
     5701 ccatgcgaca tcgagttcgt cgcccacgtg ggtcgtctgg ttgtcggatc gtgaaaaaat
     5761 gagtggccct tgtgatccac ggtaccaatt gtcgcgagcg cttgccaagt acttgcgcat
     5821 ggcccagact tcgacgtgat ctcgagtggt cggcctggct tgcaggttca cctgcggctg
     5881 aaccgtgttc ctccaggccc cgttcagcat gtagccgacg tgcaggaagt ttgtgggaaa
     5941 gaaattttcg aacgtgttgg cattgccacc gcagctgcgc gcgagagttc cgccgggtgt
     6001 gacgcaattg ctgtctccgt ctcccgacgc atagtcaaac ccgatcgcga ctcgtggctt
     6061 ccaaggatgg gcataccacg tgtggccaag ccaggtgcca gaggcccagg cattaatgct
     6121 caggttgcgc tggttgtcga agccaaaccc gtccgcggtc cgcccgaatt ggtacgctgt
     6181 ttcatgcacc agatcccaat tgccatcgcg tagttcgaga cgaagcccga ccatatgtct
     6241 gagctgagag gccgacttgg gaaggtactg cccaggattg gctcgttctg gaagccggtt
     6301 gtagtagagg acgtaatacg gctcgacaat cattcgcggc acgcctcgga tttgattgta
     6361 aaacactacc atgtcgacat cgcttcccgc gtcggccgca tgctgcgcca cggccggatt
     6421 gcagacggac tgggatgtga cgtcctggtt gcacgttaaa agattcgggg ttagacttcc
     6481 gccagggtgc ccttggccca ggtcagtctc cgaatgacga aaccagccga gtttcagatc
     6541 aaaatcggtg cgggcgtaac tcaacatgac gccatcgtga gaatagccgg tattcgccca
     6601 atcgaagtgt ccgaacaaac gttgattgcc gaacacgacg tattggcggc cgaacttcgc
     6661 actgaggcct tcgacaccgg cgagattccg gatgagcgca tagcccgctc ggatcccgag
     6721 ccggcattgt cccggtcgct gagttccgca ttgatggttg gcggcgtctg cactctggcc
     6781 tccatccgtc gggttgccgc tgcctcccca tgtcgccgaa tcctgaagtt caacgtaaaa
     6841 attgagagtg ggcgaaggat catacccgag gccgagccgc gcccattgct gaatgaagaa
     6901 gtcgctggcc ttggagccat cgtgggcgct ggtgccggat ccggagaggt tgaggctgtt
     6961 gcaggcgcca ttcataggag gtcctcctcc aaaacagacc ccgctacgcc attccggtct
     7021 gacccgcagg tcggcgcgca tccagaaatt ccggatgtca aaggcagcag ggggaggcag
     7081 tagtgcgacg gggtcagtgt cgatcaccag actttcagcc gacgagaaaa gcgccggtga
     7141 ggtcggccca tagccgacat gccccgcttc ggccatgagg gaagtcgggg caaggatcgc
     7201 gcccgatgga agtgtgaagg caaaccacaa caacaggaat ttccacatgg cgaagatgcg
     7261 gcgtctcgac agtcatggaa catagaatcc tccatttccc caagtgtcgg ggcgaatagg
     7321 ccgggggcca cttggtagcg cacccccggc agattcactg ttcgacgaaa cgaacgacga
     7381 ctagaagctc attcccgccc gctcttccat gttgcgcatg aattcctcgt gctctttcac
     7441 agtgtccgcg cctttcgccc gaatgtgccg atcccgcgcg tggaattcat cgggtgtcac
     7501 ccgatagcaa aaatcccaca aggcctcggc gctatgctcg tcaatgccgc ccacgcgtgc
     7561 ctcaagcatg accatcaagg cgttgacgcc gtcatacgtc gcacgtcgat tgccggaccg
     7621 gtgcaactct cgtatcttgc tcgtctgttc cacatagggt tgaaaacttc ctggaatcag
     7681 atttttttcc gccatggttt gataggtcac cacttgaccg agcaactctt cagcccaggc
     7741 ggcggatttt tgattactgt tccatacgac cggttcaggg ttattcgaaa atgcctgcgc
     7801 ctgggacagg tccagcaggg gccaggctcc gatcaggaac gctgtcgaat acagtgcgat
     7861 ggtgttcttc atgtgatcct cctcaggtag cccggcctgg aggccggagt gatgtgcatg
     7921 cttgggcaaa tggtcatcga tggttgctgc ccctacatca gcaacaccca tgcctcggag
     7981 aacgccatgg attgcggcta taaccttttg atttcctgag ggagcagtcg tcaggagcgg
     8041 atcgaggggt atgcggatcg tgattggcaa cggtgggcgg tgaaatgcaa cggttccacc
     8101 gcgagggaat tgcagcaatc agttggagga gccgcggtgc accgtttcga cgagatcaca
     8161 gcggtgatga agaatgttta aggaagagga cagacagcgc tggcaaggta caggtgatcg
     8221 aatgcgttcg tccatgcagg ggtacaggtg tcgcttctat cccgccggca tttccccagc
     8281 catggccgcc gtagatcgcg gcatcgctgt tcaagagttc tttccagtat ccaccgcgtg
     8341 gcgcgccgag gcgataattg ggacgcggca caggggtaaa attgcacacg acgagaatga
     8401 tctcgtcagt ggcccggcct tttctgatca gactgatgac gctcgattcc gcgtcccggc
     8461 agtcaatcca ttcgatgccg gagggatcga catcgccttc atggagtgac gattcccggc
     8521 gatacgtgtg attgagatcc gtcacccacc ggtgcacccc ttgatggagc ggtaactcca
     8581 gcgcactcca ttccaactgg ccatcatggg accattcagc cggttggccg aattctcctc
     8641 ccatgaacac gagtttcttc gcggcttggg agtacatatg tccgaagagc agacgaaggt
     8701 tggaaaattt ctgccattcg tctcccggca ttttccccag taaagagcct ttcccataca
     8761 ccacttcgtc atgcgagagc ggtagcacga agttctcgtg gaaggcatag agcatccgaa
     8821 aggtcaggtt gtgatgatga tatttgcgat cgatcgggtc cttttgcata taggtcaggg
     8881 tgtcgtgcat ccagcccata tcccatttca atccgaagcc caggccaccg aggtatgtgg
     8941 ggcgagagac caggggccaa gcggtggatt cttccgcaat ggtttggacg tcgggaaagt
     9001 gccggtacac ttcttcgttc agccgcttga ggaaagcgat ggcctcgaga ttttcgcgac
     9061 ccccatatcg attaggaatc cattcgcctt ctttccgcga gtagtctagg tacagcatgg
     9121 aggccaccgc gtccaatcgc aggccgtcga agtgatactt gtccagccag aagagtgcgc
     9181 tgctaatgag aaagctgcgg acttcattgc ggccgaaatt aaagataaac gtgttccaat
     9241 ccagctgaaa tccttgccgc gggtcggcat gttcatacag atgcgtgccg tcgaaaaacc
     9301 ccagtccttg ttcatcagtt gggaaatggg acgggaccca atccagcaga accccgatcc
     9361 cgtgatcgtg caacgtatcg atgagataca tcaggtcctg cggtgtgccg taattcccgg
     9421 tcggtgcgaa atagccggtt gtttgatagc cccacgaacc gaaaaaggga tgatccgtca
     9481 gcggcataaa ttccacatgc gtgaatccgg tcttctgcag atactcggcc agcgggactg
     9541 ccagttcgcg ataactgagc gaccgattct gttcggcggc gatgcgtttc catgatccga
     9601 catgcatttc gtaaattgcc atcggcgcgt tcaatgcatt ggcgcgcccg cgtgtgcgca
     9661 gccagtcgtc gtctgtccat gtgtaatcga ggtcccagac aatcgacgcc gatttcggcg
     9721 ggatttcatt aaagaaggac agggggtcgc ttttatcgac gcgatagtcg gcatggcgcg
     9781 agtggacatg gtacttgtat aacgctccct tggcgcactc ggtgatgaac ccttcccaca
     9841 ggccggagtc tcctcgtgga tggaggatgt gggcgtcgcg attccagtcg ttgaatgagc
     9901 cgatgaccga cacctgttct gcattcgggg cccacaccgc aaagtaagtg cctgcctctc
     9961 cgtgagaaac catcgggtgc gcgcccagtt tgtcgtacag ttgaaattgt gtcccctcat
    10021 tgaagagata gacatcatca tcagtcagtc tggtggttgc cgaaggcccc ggattctgtg
    10081 caaggttctg tggactcact ggttcacctc gtctctcacc tgtccgcctg aacggggaat
    10141 ggccccatcg ttacgcacat cgtcgttgtc agcatcctca atggcgatag aggcactcat
    10201 cgagtgtagg aggccggtga gggggatgcc cacccaatcc ggtctattat tcagctcata
    10261 cgtcagttca tacatggctt tatccagcat ctgcacatcc aataagaggt tgaactcgtc
    10321 cggtgattga ggggaaaacg gcgcctgccc tgcggtagtt cgatatcctc tcagaaacgc
    10381 cgtgcgcacc gttcgatacc agtgctccat ccacgaaacc aagtcgagcg acgccggtag
    10441 gtgagggcgg cgtgcttctc ctgtgcgcaa tgccacatgg gcggcgtagt gaaaggagcg
    10501 aatcattccg gcgagatcca ccatggggag gcatttcgcc cggcgctcag ccaaaggctt
    10561 tgccggttct ccctcaaaat cgatgatgac aaaatcttcg cctgtgtaga ggacctgtcc
    10621 caggtgatag tcgccatggc accgaatgcg caacgcgctc aagggttgcc gcgccagtgt
    10681 tgctaaccgg ctgagaagcg gagactcctg ttccaggacc ttttgagcct gttcctggtc
    10741 cgtcacagag agtgaaggaa gtcggcgtcg cagtaatgcc aaggcctgag tggcagactg
    10801 ttgcatggcg tgaacgcgcg actgtacata tgacgtggca cagggctccg gcgtaaacgc
    10861 ggcctcatca ctcggttgtg cgagtgcgat atggagttcg cccgtacgtt ggcccaatag
    10921 gtgtgccgaa tcacggtagc ccaaaagaag atcactggtc gtgggttcct gattcgatcg
    10981 gtcatgcgcc ggctgcccag cctcttcctt cctgtgatcg agcgcgagga tgccatttat
    11041 gtagcgggac agttgttcca gggtgtaatc ccaggcattc cctcgattgg cgacaaaggt
    11101 ttgtgccaga gcgagcgtca tcggctcgcc agcctcttgt acgtattcca gtgcaccgat
    11161 gaggctgggg gagtgtgtaa agtgtcgtgc cgtaagggca cggccgactt ccaattctgg
    11221 attcatgccg ggctcgattc gtcggtacag cttcatgatg gcacagtcgc caaatttcac
    11281 cgacgtatta ctctgctcgc ctttcagaac ggaactggag gcggcatgcg cnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcaccgacg tattactctg ctcgcctttc
    11461 agaacggaac tggaggcggc atgcgcgcag tgggacgctt catcaaacaa ttcggtagcc
    11521 gatccgacca acgtgccgat tgatctccgg aattgcccgc gtcggcccat gctgtccagc
    11581 agcgagtacg cgcagtccgg atgccataac gcatcgtaca gaattccagc gagtgccgtt
    11641 ccgttctgga ggacagtcag tgccccgatg atcgcctctg gatagtcatg tttgacgcga
    11701 tctgcctccg catcaaaagc ggccgtcatc ggcagtgtgt acctgtccgc tcctccttca
    11761 aggtaggaca cgtcgatcaa tgccaggacc atagtgccgt ccccatgttc ctgctgtaga
    11821 acgtctgcaa gcttggccga gcgaatcgtt ttcgctttcc cgccaaacca tcgcgaggcc
    11881 gtaaggaagt ccggcagcag cttctcgatt tccgcccgtg acgagccttc aaaggcgact
    11941 ttccaattgt cccgcacggt gatcatgaga ttggtatgcc tgcgcaacgg ttagaggtaa
    12001 taatcaaaat cctcttcgcg ccgaagattg cggcgcactg taaaaatttg tgccggcact
    12061 gaatgtgggt cgagctggac ataatttcga ggtccctgcc acacgtaata ggcctgcgtc
    12121 aggagatcat ggacttgaaa cggttggttt gcctcgatcc cgagagccgc cagattcaat
    12181 tccatccagc ccgaatgaac atggtgcggg ctgagattca ctacaatgag aacgacattc
    12241 gagccgtcgg ccgattgttt gctgtaggcc aggagatact cgttgtcgat ggggtgaaac
    12301 tgcagactct cgtcgcgatg tagcgcagga ttctcgcggc ggatacggtt gatcaaactg
    12361 atgaattctt taaggctatc aggtcgattg atatcccatc gccgcacttc atatttttca
    12421 gaatccaggt attcctcact gcctggtttc tgcggacggt tttcatagag ctcgaaggcc
    12481 gggccataaa tgccataact cgatcccaag gtcgccgcca acgccagccg ggccatgaac
    12541 accggacgcc cgccgtgctg taaatgctcg gtgaggatgt cgggcgtatt cgtccagagg
    12601 ttggggcgaa aatattcccg tactgaggag gtcgtgagct cggtgaaata gtcggtcagc
    12661 tcctgcttgc tgttgcgcca cgcaaagtag gtgtacgact gcgtaaaacc tatcttcgct
    12721 aagtgataca tgactttcgg cctggtaaac gcctctgcca ggaagatcgt ttccggatag
    12781 gaggctttca cgtcgctgat gagccatttc cagaacagaa aaggtttggt atgcggattg
    12841 tccacgcgaa aaatccggat gccgtggtcg atccagtact ggaccacctc gcgtagctct
    12901 ttccaaaggt ctgggtacgc gtctgtctcg aaatgtaatg gaaagatatc ttgatactgt
    12961 ttgggcggat tttccgcgaa ttgaatggtg ccgtcagggc gtgttttgaa ccattccgga
    13021 tgctctttga cgtaaggatg gtcgggtgag cattgaaagg cgaggtcgag ggcaatctcg
    13081 atgccgttcg tctgggcggc agccacgagt tgctcgaaat cgcccaggct tcctagttcc
    13141 gaatgaattg cggtgtgccc gccatcgcga gatccgatcg cccagggact gcctaccgaa
    13201 gacgagtcac ctcgcggatt gttatttcgt cctttgcggt gggtttcgcc gataggatgg
    13261 atgggcggca gatagagcac gtcaaatccc atctccgcga tgtaggggag ccgtgactcg
    13321 caatccttga atgtgccgtg gggctgttct tcgtctgtcg tcgaacgggg aaacatctcg
    13381 taccaggcgc tgaatcgcgc tttgggccga tccaccacga cgatgagctc gcggtcatat
    13441 gtactcatca gggcgtgatc aatacagtcg gccatgatct ctgccgtgtc ctcttcgagc
    13501 aggaggtcca gcagagcctg cgaagcgaga tcagaacgag acacccttgc ctggatgtcc
    13561 ttcaggcgtc gtcgggatac gtcaggcgcg ggagcggtcg cctgttcgat caagtgcttc
    13621 ccgatcaaga tctccaccgt gacatcctgc cgagcccgca gccgtttacg catgtcacgc
    13681 tgccatgtcg cgaagggatc cacccatccc atgatgccgt agcggtattg gcccagttcc
    13741 gtgacatcga acgaggcacg ccagcggtca ttgaccaggg ggctcaacgg cgtagttagc
    13801 cattcctcct gacgttcatg acgggaacgc agaatgcctt ggatcacgtc gtgtccgtcc
    13861 gcgaagaggt ctgcttcgac gatgatcgtc tctccgacca ctcgtttgat cggatagcgc
    13921 ccgtcgtcag cttgtggctc gacatgttca atggccacgc gcaaccggcc ctcgcccaag
    13981 acaggggacg aggcttgctt cttccggctc gccggcttgg acggcacgtt tcgccgagca
    14041 tctgtaggcc gcgctttttt cataaatgtg ataactggtc ggccttgtgt tcgtgttgac
    14101 tgaagcggcg ttcaagaatc tcccgcatga gatgccgcgc gcccaagccc acggccaggg
    14161 ccagggcgag cacgacaccg ccgaaggtga tggagaacgc ggcgatgaca atttccttcg
    14221 caatgcctaa ctgggtgagc accatcgcgc aggtgaatag cagcagcccc caccgcacca
    14281 gtcccgcaac gacgcgcgct tcctccacct gggcgttcac ggcggcgatc aatgcggcct
    14341 cggcaaagaa attggcgcac agtactccca cgaggagcac aagggatgcg gccaggacat
    14401 tcggtaagaa cgcgacgatt tggctgatca aattcgccgt ggccggaagg ttcatggcat
    14461 cgacgcccat gaaggcaaat aacaaaaaga cgatccagaa cagcacgccg ctgacgacca
    14521 gggataccgg tcggcgaact cctgcctggg agagcggctg aacaatgccc aatcgttcgc
    14581 aaaacgaatc gaacccgaag gtccggagaa cccggagtgt gagcactttc accgaccagg
    14641 cagccaacag acccagactg agaaacgtta gcaaggcgag gagccgcggc aacacgagca
    14701 cgatttgcat caccgtatca cgaaaaccgg cgaccatcgt ctctctccac aggtcactca
    14761 tcgcgccacc tccattgctc cacgaggtat ggtttcgccg cttcgaactc gcggcatact
    14821 gtttccaccg tgcgctccac ctcgccggca tccccatgct gagtttctaa aacgaacgag
    14881 gctgttcgtc ccaggtagag aggggtcagt gctttcagca tatgttcccg atgcagcgtc
    14941 tggttgtgat acgccaagga aaaatcgtaa acagtttgca cccagaggga agggggaaag
    15001 cggaactcct ctggatcctg tatgtcgagc gcgatgatct gtcccacggt gtcccgagat
    15061 aaaatcagct cccagagtgg aagcaaatcg cgcaatccct gtttgaaccc actgatcatc
    15121 cgtccggtgt gcagcgggcc gggatcgggt gccgactccg gctcgccgta gagcggcact
    15181 tcactggacg ccttaattgc ctcccacgac gactcgtacg cttccatcga atggaagatg
    15241 cctccaacgc tctgggcgat cagcaccgac agatcaacgg cgccaagtgt gctctcctga
    15301 tctctggtgc cgagacaggt atggcacaca cgatacccgc cggcgagcgc ggcnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcatcgc aattcacgag cagcgcagac
    15481 gcatggggga agaattgccg catcccttcc acaatcgatt tcaggacagg ctcgatggat
    15541 ccggcattgt tgagggtcaa gaggccgatc atgatgtcaa tagtgtccgt cggccactcc
    15601 atgtcggttc tcgacacgct cagctctggg ctgggtgcca gcacctccgt ctcactcttc
    15661 ccgccttgcg gcttcgacat gacggtggtc atgaagtctc catccctctc acgctgccgg
    15721 taggagtttg agtagggtga ggtaatcacc cagctgccgg gtaatcagga aattgcgccg
    15781 tacatgttct cgcgccatgg cccccatcct gttcatcagg ccgggattgt tgagcaaatg
    15841 ccggatacgg aatgccgcgc cctctacgga atggaccaca taccccgtaa cgccgtccac
    15901 gatctgggcg gcaataccgc cggcggtgct gccgacgacc ggtttgcctt tccacatcgc
    15961 ttccgacacg gttaacccaa acccttcttt gacggatttt tgcaagacca ccgtggcact
    16021 ccgctgcagc gcattgatct ccagatccgc ttccggggga agttgaatga catgaatgtc
    16081 gggatcgcgt gccccggcct cccggagctc catgaggacc gcttcgccct ccggatcgtg
    16141 catgaccccg gaccccgcca acacgagctg gcaatcatga tgacgcttga cgagttggta
    16201 ggtgtgaatc gcacccaacg gatccttgaa gcggtcaaat cgtgccactt gcaagatcgt
    16261 tggtttcacc cggggaatgg cgagtcgatc cagcacctgc gcgatctccg atcggctcat
    16321 gtcacggttt ttcggactca gcggatcgat ggacggataa atgagaaact ttgggatcgg
    16381 tagtcgttgg gcaaatccgg acagcgagaa aatggcggca tcatatttga ggacgtaacg
    16441 ccgaagaaac gcccaggccg gacgttgagg ctgggcgagg tccaagtggc agcgccacaa
    16501 ccagcagcca ttggtccgat agtcgaccag tcccgccggt tgatggtcgt gaaccatcac
    16561 catgtcggct tcgagtttca gcgccttggc atttcgctcg atgacattcg cataggtctg
    16621 gtacatgtcg tcagtcagct tgtcgtctcg gccctgtaag gcgttcagca tgcgcgtgac
    16681 caccacaaag tactcttgtg atccggccag gacttcccat cgggcctcaa cgcccaacga
    16741 caccatcatc ggcacgagtc gccgcaacac ctctgacatc ccgcctccat acctgacggc
    16801 attcagatgc aggaaactct tgccttgtac gaggtcgctc agccggtaga gaaaatccac
    16861 cgtccctttc ggtgatacct cacgatattc gtccaatgcc gaatgcactc gatccttctc
    16921 cttacgtggc gtgagatccc gggcgattca tgttcgtcaa attgcgctcc attccatgct
    16981 gtaggcggtt gtcggcgacc gcacggccgg caagtgcaac caatacgagc tatagggccc
    17041 gagggtcagc acatacgggt gctcgtgaat ggtggggaag cgtgactcac cgagcaattc
    17101 ttcgggcacc acgtctttgt agcgactgag atccagctca acggcctgtg aggatcccgc
    17161 caggttatgc acgagcagca aggtttcctg ttcataggca cgaatgtacg cgaggacttt
    17221 ttcattggcc ggactgagga actgcaacgt tccgcgccca aacaccttat gcttcttccg
    17281 gatcgcgatc atgcggcgca tccaattaag taacgaatgg gtggcttgtt tctgcgcgtc
    17341 gatgttgacg gcctgataac cgtagacggc atccgcgacg acaggaagaa acaattgcga
    17401 cgggtcggcc ttggaaaagc cggcattacg atcggcggtc caatgcatcg gggtgcgcac
    17461 gccgttcctg tcgccaaggt gaatgttgtc gcccatcccg atttcatctc cgtaataaat
    17521 gatgggactg ccgggaaggg tgaagacgat actgttcaaa agctcgacct tgcggcgatc
    17581 gttatccagc agcggagcca ggcggcgggc aatcccgata ttgcgacgca tctgcggatc
    17641 gcgagcgtag gcgtaataca tatagtcgcg ctcttcgccg gtacacattt ccaatgtcag
    17701 ctcatcatgg ttccgtagaa agaggcacca ttggcaggct gatggaatgt ccggcgtatg
    17761 tttgaacatg tcgacgatcg gctgccgatc ctcgctccga acgcccatga acaagcgggg
    17821 catcaacgga aaatggaagg ccatgtggaa ttcatcgccg tccccgaagt aggggcgcac
    17881 gtcgctgggc cattgattgg cctcggccaa taagacccgg cctttgtatc gctgatcgat
    17941 gctgctgcgg atgtctttca ggtactgatg ggtttccggc agattttcgc agatggtgtc
    18001 ttcgcgctcg aagaggtagg gcactgcatc acatcgaaac ccgtcgaggc cctgatcgag
    18061 ccaatactcc atgacctgga tcatggcccg gcgcacctcg gggttgtcgt agttcaggtc
    18121 cggctggtgg ctgaagaagc ggtgccaaaa gtaggcctgt gcctcatggt cccaggtcca
    18181 attggatttt tccgtatcga cgaaaataat ccgtgcgcga ggatatttct gatccgtctc
    18241 gctccagacg taatagttgc gggtgggggc atccggagaa gctttggctg cctgaaacca
    18301 ggcatgttgg tcggaggtgt ggttgagaat caggtctacg atcacccgca tgccgcggcg
    18361 gtgcgcctcc tccagcaaac ggcgaaaatc gtctgtcgtg ccgtaggatg cggcgatgct
    18421 gcgaaagtcc gcgacatcgt atccatcgtc ccgcaagggc gagggataga aggggagcag
    18481 ccacaagcaa tccacaccga gccattcgag atagtccagt ttctgaatca agcctggaaa
    18541 atccccgact ccgtcgtcgt tgccatcgta gaaggcgcgg acgtggagtt cataaaacac
    18601 ggcgtctttg taccaaagcg gatcttgatt caacatggat cagctctttg ccgactcgct
    18661 ggtcaggaaa tcatcacatg cgctgatgag tttggaacgc agccgctcca gactgccctg
    18721 gtagggattg atcgagcgga tgcggtcagc cagattcgtc aatttcagac tggtgcggag
    18781 ccaggccgaa aaatcgtttt cggcccgtcg taatcgaaag tgcgcttcaa acatatggta
    18841 gtagatgctg ctcacatcga tgtccgtcac gatgtcgcgg aactcctgca gcgtgcgcgc
    18901 ctcgagaccg atggggactt caaggatcct ggagcgcttg aaatggaatg gctggccgaa
    18961 caccacccta ggaatgatgg ggctgcggga aaggtggtcg tcgatgatag aaatcagttc
    19021 ctctcgcaag ttttccaaat ccgggtagtc gaacggatca agaaccgcaa gacgctcgcc
    19081 cagcacgtga tccccgattt gcatgacgac ccattgcgca aagtcattgg gataggtgcc
    19141 ctcaatcagt ccatggcgga ggaaatagct gtgtgtgtgg aaatggatgg agtccagcgg
    19201 cacttcttcg atgagctccg ccagttcctt ttcatcgtcc gcctgatgcg cgatactttc
    19261 ctggagttcg ctgcagccga taaaggcaaa cggggcatct gctgtaagga ccacaatcca
    19321 gactccttca gagagttcag ccgtaagtca cacgcatccc gcgtctctcg gacaacatct
    19381 aatcctcagg atgcgtccct gatgggttca ctataaaact tcggccgcgg gatccttact
    19441 agtgaaacac ctagatggag agagacgaag tccagcgaca tactggtctc tgactgagtg
    19501 ggtatcgtgt agatggagtg atgactcagc catggctctc agaacagcat gcctcgttcc
    19561 gattgacgag tccttgaatg tacgatgcgg caggaactca gtagggcgtc gtgtcgcaaa
    19621 gtacaccagc gacgggcagc cactgtgcca ttttgaatgt gaagcgggac atctgttcca
    19681 ttctgatgaa acgtgccgat attattttgc ctgcgattgt ctgccgcgct atgcgcggca
    19741 gggtcgctca cctgcgatgc cccagcagta aagacgctcg gtttccgcga ggccttaaac
    19801 gtacctgcct gacagcgaac aagacccttc caccacgctg atagacgcga ttttcttcac
    19861 aaacttcagg gtgtcgtgta ccgtacaagc atcgactcca accctggtgt tcggagtgtg
    19921 gatgcgcgag tcagtgctgg ggcatccaac ccttggtcta gcgtctacat gctcaatccc
    19981 tatctattgt tttggagtat cggcctgctg ttttttgtcg ccgaatggct caggccggcg
    20041 agaaagatta cctatcgtgc cgtcttctgg cgcgacctcc tcgcgctggg cgcgtataac
    20101 ctatcgttcc tgctggtcgt gcaggtgacc gatcggattc cggttccgca gtatctcccc
    20161 gttgccctgt ataatctgcc cacagccgga aaactcttgt tgttttacat cgtcgaagac
    20221 ttcggcctct attgggtgca ccgtctcatg cacacgaggg tggtttggcc cattcaccgg
    20281 tggcatcatt caccgacgtc tctgtactgg ttggcaggca tacgcaccac gatcccgcat
    20341 atcgccctct tcaatgtgac ctatgtcgcc gcgcttccgc tccttcacga tgcctcaggc
    20401 tgggcgttcc aggtcatcat ggtggaacat attgtgcgta acaattggat gcacgtgaat
    20461 gtcacctgga agtcgtcctg gttggaatgg gtgttcgtca ccccccgcta tcatcacatt
    20521 caccacagca gcgatccggc gcatcaagtc aaaaatctcg gcgcgttgct cacgatctgg
    20581 gaccggttat tcgggaccta ctacaatcct gacgatgtga ctggtgaatt gaagtttggt
    20641 ttgagcgaac gtgtgaaccc gatacggctt gtcatcggcg tctagcacgc ctattcagcg
    20701 ctagagagac ctgctcagac gacggcactt ctccattacc tgaccatcca gccacgcccg
    20761 tggtcgagtc tcacgaatct aaacaacgtg tgctgtagga agctcgtgga gcgggtgcga
    20821 ggttattgag cgccgttctc gccgatcacg acgaactgct tgcggaaccc gacgactttg
    20881 gcgagatagt cccgggtctc ttggtaagga agcccgctgc ggagccggtc atagagggtg
    20941 gatggctgaa ggccgttgat ctgttgcagg gcggtacgct gatctttggc aaacgttttg
    21001 aacacgttgc cggcgccggt attgtaggcc gagatgacgc agtattcacg tgacaccatg
    21061 tccgtgacgt cgtccaattg agaataggtc aacacgttga gatacgcagt gcccagttcg
    21121 atgttattgt ccggatcaaa aagataatcc cgcgacgggg ctttgtcttc ccctttcgct
    21181 ttccgatacg catcccgccc tccgctggtg ggcaccagtt gcatcaaccc gtaggcgggg
    21241 gcggagctga cggcaaatgg attgaaatta ctctctgtgc ggataatcgc aaacacgaga
    21301 ctcggactga tctggtaccg ctctgcgaac tgcgcgacca cggtgcgata tttctcggcc
    21361 tgtttgtgcg agaaattggt caccatgggg atgttgacga gatgtgcggt tttggcaccg
    21421 ttctcctgct cgactggccg ggtcgtgctt ttggacagga gcgattccgc gaattgttcc
    21481 gcttccgtcg gcgtacgcac cggcttgccg gcctgattca cgaccagacc aagaagatac
    21541 ggttccttat cgccggtcag ggtgatttct ttgtccgaaa acaaatcgac gctacgcgga
    21601 tcgttgggag ttaggagcgt cgtgacgacg gcattcttca ggcttgcacg cggatccttc
    21661 tcgtcgagcg tctcgatcag aatgttcccg gcatcgaagt ccacgatcgc ccggctgcga
    21721 tagttctggg tgtatttcac atacttcttc tgctcgggta atttgacttc cttggtgccc
    21781 catttcttgc cgacctctcc cgtcaacgcc gccatcaatg cttggaagtc gcgttgaatg
    21841 gctcgcagat ctcgaagcaa tgcctggggg tctcggccgt actgctcgac ccgttgccga
    21901 gcgatatcgg cagggtcctt cccgtggacg atatccagaa cggttctgcc ggtcgtgctg
    21961 ccgacagccc gctcggcagc gccgagcatc ttatcggtgg tttcacagcc ggaaaataac
    22021 gtggggacag cgagacttag cagcaacagg gacattcggc gagtgcgcat ggcgggagta
    22081 tacagaaggt gtggccggga ggaaaagaga atttccgaaa ccgtcattat tcgcatggac
    22141 agggcccgtt tcctccggtc ggccatcggg cataggccag taaatccacg tgcttgacgc
    22201 ctggttttgg gggccagtac cataccccat cgccgggatt ccgggatgaa aggcggaggt
    22261 atggggaacg gactggtggg tatgaacaca cgtgcttcag ctgcggtatg gtgctgggta
    22321 attgtgctgt tgatgacgtt ggccccaagc cccggccgcg cggcccagac gccgaccgag
    22381 gccgtgcagg tcacgatgaa ggatctgctc tacctgctga ccgaactcaa ggaaaccagc
    22441 cgatccgctc agcgacagtg ggaaattgaa caggtggtgc gacgcgcctt taattatgaa
    22501 gaaatggctg aacgggcgtt aggggagggg agcgaaaaga ccacgattgc cgatcgccgg
    22561 caatttaccc gactctttgt gcaggtcctc cgtgacgact tagccgataa gctccgcgat
    22621 tattctcttc ctcaggtggc ctatctctcc gaacaaggcg atggcacggg aactcaggtg
    22681 ctcgtggcgc ctgcggggcg agaggtcgat acgaagatcg aattttctgt cgtggagcgg
    22741 tccgggcgtt ggctgatcaa tgacgtcagc atcgacggtg ccagtattgt ggcgacgtat
    22801 cagacgcagt tctcccgcat cctccgcgaa ggctctttct ccgaccttat ggaattcctc
    22861 aagcagaaag ccgtggttgc ttagatcaga tcttccggac ggctgccctc atatactccc
    22921 acgattgtcc tcatgcctca tcggccgttg tcatcaacgg cgtgactccc actcatgccg
    22981 aacggatccc tcggcctccg gccgaattgc cgccagacat tgttcccgga ataccgtgtc
    23041 gctgcgcgag ttagtggtgc gcgtggacct tcagcaagcc gtactcatag gcccgcaaca
    23101 gcagtttctg gcggctgttc acatcgaact tcccgaaaat actggtgagg tgatggcgga
    23161 cggtgacgct ggaaatacag agccgttcgg caatgtcctt attcgagagg gcctggccaa
    23221 tcagatagac gatctcccgt tcccgtccgg tcaatgcagt gtcgggagat gtcgacgaga
    23281 ggcttggcgg agaatcatcg tcgatggatg attccgtgca gtgcaatctg cggcgcgtcc
    23341 ttcgttcata cagggttgtg gcgtcgggga ggccgcagac gtggctgatg gtagcgagca
    23401 ataccaccgc cggttgaatg gtcaggacaa tgccgtcnnn nnnnnnnnnn nnnnnnnnnn
    23461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23521 nnnnnnnnnn nnnnnnnttc cgtccgagga gaccttagag cgatgacacg gcggggcttt
    23581 tcgtcgcttc gcgccggcaa tactcataac agctggaaag aatgaggtcg cccgagaaac
    23641 gcacggtgcc acagacctgt cccataagaa gggcccagtc ggccacatgg gcggccaact
    23701 gggtcggagg cattccctgg gtacgaaaca gattaatggc caccgtcgag aggactattt
    23761 tgagatgtga cagggaagga ccgtaactga acacatattc gtgttgtcct gtaaagagga
    23821 gttgcacccc cgttctagcc ttcatgccac gatgaccgca gcggggacaa aagaggcatt
    23881 caagtccgct gtcggggtca tacccggccc gaacatccgt ttccatcatt tggccacaat
    23941 gggtcgcagg gcacagcatc atgataatct ccctgaagaa taatcggtgc attgcgcggt
    24001 tgtcgtctca ggcgctatgg agcgcgtgag ttcccgagcg gccggaacct tctccgcttt
    24061 gcggggccga ttctccctgg acgatccgtt ccgcctcgag ccaatcttca tagtcgtatc
    24121 cgtcctgctg tcctcgttgc tgatatctgg catacgccag cttttcgatc ctggttctga
    24181 tgtcctcggc tgtatccgtg gtctcggctt gcggcgacgg ctggagtcga tcctttcgct
    24241 tgggaatcga cctgctggtg gcccctcggg agatgtttcg tatgggttcg tcgcctgcag
    24301 gtgcctgtgc gttacgttct ggcatgatga ccactccttg ccgatcaggc cggccgctga
    24361 gatgttatcg atgaatcttg ctttactgaa gtcgtacgca gggtatgccg agcgtcgtca
    24421 ggagcaccgc ttcgacgggg tgttccagag ggggactggc ggatttcgct gagagcgtat
    24481 cgccggatcg ggactctccg ctcaagacgg gatggaccgg ctccgagagg gcaagaacgg
    24541 gtgcctgggg acagagggcg ttaagggcgc ggagtgcgtc catccccgga gtgtcgcagt
    24601 gggaaccgcc tgaatcgtga ctgaaaagaa tggctgccgg ctgaatttcg ccgagttgcg
    24661 ggagtgtgga ctcgaggtct cgcacgagca ccgcatgata ccctctcgtt gccaggcaat
    24721 cggccaattt gagcccgaac tgcatcgcag aatcgacgat catgacgcgt gatcttcccg
    24781 cttgcttgac agggtgcatc gtgggactca tcgttggccc tccttgttca tgatctggtc
    24841 aactgcggct acccccaaag acggtttccc ggccgtgacg gggatgagat ggcgcaaggt
    24901 gctcgatccc gatccggcga gggacaccgt gtactgaaat tcaggcaggg gacgcagaac
    24961 gagcgtgcct cggcgggaca ggatgtccac ggccatgaat acctggttcc aggtacaggt
    25021 ctggagttgc tgcgccaagg cttccagcgt gcaggctccg cgccgtgtga gttctcgggt
    25081 aatcaatccc tctatctcag ccagcgtagt catctgatcg ttccttttcg gtactctcct
    25141 gatgtccggg cagggtccaa ttgtacggcc ttcgctgctc cggccttcgg gggacgcagc
    25201 agactcaatg tgacgcgaca gtgctaggtg tacctcttga gaggcaggca cgccatcgcc
    25261 ctttgcgcaa aatgcgcgtc gccgttctcc cagttctggc tgtcgccgtt tatgctacga
    25321 attggagagg agaggcagga acaacgtctg aacggagtca ggacacgaga cggttgacca
    25381 gacgattttc gagggcgtaa tgggtcaatt cggtattgtt cttgaactgc attttttcga
    25441 ggattcgcgc acgataggta ttgatggtgc tgatgcccac gcacaggtca tccgcgatct
    25501 ccttgaggct tttcccgacc gcaattaaac acatgacttg gtattcgcgg tcggagagca
    25561 actcgtgctg aggtcttccc gcctcctcat tgaggttggc gacgagtagt tccgccatgg
    25621 aggcgctcac aaatttcccg cctcccagga tcttcttgag cgcctggacc aactcgttcg
    25681 gggcactgtc cttggtgagg taccccgagg ctcccgcttt gagcattcgc cgggccaact
    25741 gatcttcggg gtaggcgctg agcaccagga ccggaagttt cgggcagacc tgcttgagct
    25801 ctttgagcgc atcgaggccg ctctttcccg gcatgccgac gtcgaggacc acgatatccc
    25861 aggtgaattt ccggatccgg tcgatcatct cctctgcatt ccgggcctcg ccgatgacgg
    25921 cgccctggaa ttgctcgttg aggatttgtt tgacgccttg tcgaacgacc gcgtgatcat
    25981 ccacgaccag aatttttgtc acgataggct ccatctgttg cgtctggtcc aagaatggac
    26041 cggtcatgat gactcaaatc tgttcccggc ttactcctga gccggctggt caagcgggat
    26101 cctgactcgc acgattgttc ccgatccggc attgcctgcg aacgtgatgc tgccgccgag
    26161 cagtaaggcg cgttcgcgca tgccgaccaa tcccagggac tggggatccg ccaactcggt
    26221 ttcggtgatg ccgcgcccgt tgtcttgcac ttcaagcacc agcccgccgc cctgttcctg
    26281 gagcgtgatc ttgacgacgg aggccttcgc atggcgcacc acattggtca ggatttcctg
    26341 aaaaatccga aacatcgcgg tcgatgcggt ctgcgatagg cacgcggacc gcagatagat
    26401 atcgagtcgg cattctatgc cggtgcgtga ctgaaattcg tgcgtctgcc attcaatggc
    26461 cggaatcagg ccgagttgat cgaggacgat ggggcgcagg tccgtggcaa ttcgtcgcac
    26521 ggactggatc gtggcgtcga cgagaccgga aatggattcc aggcgactgt gcacagtggg
    26581 gggcacactg gggagctgat cctgcaacaa ggagagttca agtttgacgc cggtcaacgc
    26641 ctgcccgagt tcatcatgga tctcgcgcgc gatgagaatc cgttcttctt cccgcaccga
    26701 ttccaggcgg gcggtcagct ttctcagctg tgcatgcgag gcggtcagtt gctgctccgc
    26761 ccgtttgcgg tcggtaatgt ccagaaagac gaccacggcg cctgtgacct tcccctgttc
    26821 aatcacgggg aaggaggagt attccacggg gaatgacgtt ctgtccttgc gccagtacac
    26881 ttcatcgtcg accttgcggc ctctcccatc cacaagcgtt tcgtggatgt ggcaccgtgt
    26941 ccgatgatag atgccgccgt ccggcgagga gtgatggatc cgttcatgca tgtcttggca
    27001 cagcagttca tcgggcgaat accctaacat acgcgaggcg gcggaattga tgaacgtgca
    27061 gcggccctgt cgatcgaggc catagatgcc ttccccggtg gattccagca ggagaaggcg
    27121 atctttggcg agttgctgtc ggatttcttc cgcccgctgt ttatcggtga tgtcggtggc
    27181 tattccgcag agcgcgtaac agcggccctc tgcgttgcgc aaaggaaact tgaccacaat
    27241 gctggtgtgt gggccgtcgt catgcaacgc tgattcttcg aattgcatcg gggcgtcggc
    27301 ttgaagcacc ttccggtcgt tctcgcgaaa gaccgccgct tgagccggcg caaaaatttc
    27361 ctcatcggtt ttcccgacga gatccttgtt cgccaggtgg gacaccgttt cgaattgccg
    27421 gttggcttgg aggtagcgtc cttctaaatc tttgagaaag atcagggcgg gactgttgtc
    27481 cataatggcc cgaaagcggt tttcgctgtc cctgagcgct tcatcggtgc gcctggcttc
    27541 ggtgatgtcg cgaaagacca ccacgccgcc tttgatcagg cccatctcat cccgcagggg
    27601 cctggcggac acatccagcc agaggtcgtt accctgactc cggcgtttga gaaacagggt
    27661 cgagccggtc acggattccc cccggatggc ccgtgcgagc ggaatatcct ccgcggggta
    27721 aggggtcacg cggtcaggac gaaagatggt gaagtcctcg gaccaatggc cgactgttcg
    27781 tttggccggg ccacttccca agatctgttc tgcggcggga ttccagaccc gaaaggtgcc
    27841 ggtttcatcg gccatcacca cgccgtcagc catgctattg agtactgatt ggaggaagcg
    27901 tgtctgctcg cgcagttctt cctcaagccg cttgcggccc acggcatatc tcagcgcgcg
    27961 tgcgagaaga ggggccgagg tctgtccttt gatcagatag tcttgcgcgc cggcttgaat
    28021 gaggtggagg cctaactctt catcctccaa gccggtcatc agcacgatcg gaatcccatg
    28081 tgcggcggca tgcattctga tcagggtttc aatgccctgg ctgtcgggca aagacagatc
    28141 gaggagaatg acatcgatga gggactcgct cagacatcgg attcccgcat cgagacggcc
    28201 gacgtgcgta agggtgaact gtccattgcc ggtttgtgca aagagctcac gcaccaggcg
    28261 aatgtcgccc ggattgtctt cgatcagcag gacatggata ggccggttgg tcataccgct
    28321 cccggttgat gtgagaccgg cagcaccacg atgcccagcc agaaatcttc gatcgatcgc
    28381 acgacccgca cgaactggtc caggtcgacc ggcttcgtga tgtagcagtt cgcatgaaga
    28441 ttataactct tgaggacatc ctgttcgtct tgagaggtag tgagcacgac cactggaatg
    28501 cgcttcaggc tgtcatcggc cttgatatcg gcgagcacct cgcggccgtc cttcctcggc
    28561 aggtttaaat cgagcacgat gaggtgaggg cggggggcgg cggtgtagga accctgccgc
    28621 cgcagaaagg ccaaggcttc catcccgtcc ttgacgaccg tcagatgatt gatcaccttg
    28681 gcctctttca aggcctcgat ggtcaaacga acatctccgg gattgtcttc taccagaaga
    28741 atttcaatcg gctttaccag ttctggcacg accatcgtga tggctccttt ggattgtgga
    28801 cgtgaatcat gcaacgcctg ttgtcgcgcc ttctgagcga gtgacggagg gttgctgcgg
    28861 agggtcggga atgctgaacc agaaggtcgc ccctttcccc gtttcagact ccacccacat
    28921 gcgaccccca tgacgttcga caatcttttt gcagatggcc agaccgatgc cggttccggg
    28981 gtattccgcc ctggtatgga gtcgctggaa aatgacaaaa atccggccgg caaattgcgg
    29041 atctatgccg attccttcgt cgcgtaccga aaagagccac tcatcttctc tccgctgggc
    29101 ggatatgtgg atgcgcggcg gctggtcccc gtggaactta atggcgttgc tcagaagatt
    29161 ctgaaacagc tgcgccaact gtttggcatc gcctctgacc gtcggcagcc ggtcgtgggt
    29221 aatgaccgcc tggctttctt tgatggcgct caggagattg tccatcgcat aggagagcac
    29281 ggctcccatt ggtgtaggct caaacggctg gctgcctgtg ctgacccgag aataggcgag
    29341 caggtcctga atcagcttct gcatacgggt ggcgccatcg acggcatagg cgatgaattc
    29401 atccgcgtcg gcgtccaact ggcctcgata ccgcttcgcc aggagttggg tataactgct
    29461 caccattcgc agcggctcct gcagatcgtg tgacgccaca taggcaaatt gttggaggtc
    29521 ggcgttggag cgtcccagtt ctgccacaga tttttcaagg tcctgtcgcg ttttcaggag
    29581 ttcggatcgc tcgcgtccca atttccacga aaccaggatg ccgaatcccg tcaggggtag
    29641 gatgacgagc aatccgatgc cgccgaccgt ccacagtagc cgatcgacag gcgcatagac
    29701 ctggtcatgc gtctgacgga acagcacaat ccaatcaaat cctggaaaat cccgatagcc
    29761 tctggtcgag gcatacccgg tgacgacgga gtgcccctgg cgatgatgga cttcttcgac
    29821 aaatcctgat tgattccggt cggaggctgc ccgggatcga gagggaatgt ctaagatcgc
    29881 tgatcccttg gggctctccg ctgaggtctg tttttcgctg atgatggccc cgtcccgagt
    29941 gagcagcaac caatcatagg ggtctttgcc gtaccgtagt ctgccttcct gttcgaaaat
    30001 cgagcgaaga ttgtcgatgg ggacgcggct ggccaccact ccacgaaatt cacctcgcgg
    30061 accgtagatc ggtgccatga atcccactgc tttctgctgt gggggcatgg cagaagggac
    30121 caccggttct aggtaaatgg tgcctgaccg ccgaacggca tcaaaggctg cacggttgag
    30181 aggggggggg cctgacgcag gacgcatgtc ggtggccgcg atgaccgttc cgtcggcatc
    30241 ggtgattccc agccagctgt agtaccagta gagctggtgg taatgctgca gccgggcggt
    30301 cctctcttcg gatgaaccgt cccggatcac gccatcgttg gcaaacaact ggatatcgcc
    30361 ataccgctcg aacaacacgc gatcgactgt ctccgccacg gtcgcagcat tcatggcgag
    30421 ttcgtctcct cgttctgcga ccagcatggc acgcaggaag gagagggcgt ataacccgag
    30481 ggcggtcgcg atgaaaaagc acagaaagag gaggatcggc aggatgcgtt ccgtccaccg
    30541 gctgctacta tcgaatgctg tggtctgcac ggttgttgtc gggagcgcaa tcatgtcagg
    30601 tgattagagg gtaaagcctt aatagtttaa cctgttcgga taggcagttg cacgaggaag
    30661 ccggtgagca tccattcatg gctgcgttcg attgactccg cgccggcgtc cgggtacgtt
    30721 cagaccactc taggcgcgca aaggacagga tgttgagcat ttcgacacat gtctcgattc
    30781 cggatgagga gatcgagttg acggcagtgc gggcgcaagg cgccggcggg caacacgtca
    30841 ataaagtctc gtccgccatt cacctccggt ttgacgtcag ggcgtcctcc ctgcccccgt
    30901 tttacaaaga acgtgtgttg gagctgcggg accatcgtgt gtcgcgtgac ggggtcatca
    30961 tcatcaaagc acaggactcg cggagccagg agcggaaccg tgcattggcg ttggaacggc
    31021 tccgggagtt gatccaacag gcggcgattc cgcggatacc gcgtcgacct accaaaccga
    31081 cgaaaggctc taagaaaaga cggctggagg ataaatcgaa acgcggccag ctcaaaagtt
    31141 tgcgaagccg gcctcatgag cagtgaggcg caggaagaaa aaaaggggcg cttcgggtga
    31201 agcgcccctt ccaggacgag acgaacctcg tcaccaatta gaagcggttg cgtccgccgc
    31261 ggtcaccgcc gccgaaccgt ccacccccgc cgccaccgga gcgaggctct tgcggcttcg
    31321 cttcattgac ggtgagttgc cggccatcca tgtcggagcc gttcaacgcc gtgatggctg
    31381 ccttggcttc ttccgccgtg gacatttcca cgaagccgaa gccgcgtgat tgtcccgtga
    31441 acttgtccgt gatcacgcgc gccgattcaa cggtgccatg agcggagaac aagttgctca
    31501 gttgcgattc agtggcagca tacggcagtc cgccgacata caatttgtta cccatagggg
    31561 tctccttttg aaatgtgaca ttgtcggcga cacgagaaca ggcatatgag gggaatgagc
    31621 gggccgaagg cgcggtggta ggggcgactt gggtctgact tccgatcaac ttctcggtaa
    31681 caaacaatat cggtgatgag ccatcaacac actgtgattc tcatgcggcc cgtcataaga
    31741 tcactctgca tcgtaacaga tgaagcaggg gattactacc ggtttcaggg ctgtccaaaa
    31801 aatactttga ggcgcggcag agagctgcaa ccgtagccag acccggttct aattgcctgg
    31861 ggacgcggta cggagcgatg aggccaagcc taacttcgac agggtataga tcagccactt
    31921 cgacgggtca aaattgtacc acagcggtcc attgcgatag tcgctcgggt aggtgtggtg
    31981 gtaattgtgg tacccctccc cgaaggtcag caaggagacg agccatgagt tgcggctcga
    32041 atcggaggtg ccatacggct gctcacccca gatgtggcag accgagttga tgcagaaggt
    32101 ggaattcagg accgcaaagg tgcgtccgac gccggccagc ataaatcctc cgattccacc
    32161 ctgccatccg ttatggagga agccgatgac gaaggggagg gccaggccca cgacgacgat
    32221 ggccgcataa ttgcggtgtt gccacatggc caccggatcc tgcatcacgc gggagccgaa
    32281 tcgttcatcg ttgcagggca cgggattgag gagccagccg caatggctat accagaatcc
    32341 ccgcttggca ttgtacggat cagcgggatc gtcacaatgg gcatgatgac gcaaatgatc
    32401 ggccgtccat ttcgcgccgg agttttgcaa ggcccaccca ccggcgacca gcaggcaggc
    32461 cttgacccaa tcgggacatt caaagctgcg atgcgtcaac aggcgatggt atcccacggt
    32521 aattcccagg ccggtgatca ggtagaggac ggcgaacatc gtccaatcca gccaggaata
    32581 gccatacagg attccgtacc cgggcacgca aatcaccgag cctgcgacaa tcgaccagaa
    32641 gagaaggtag gtccagaact tatgatgcac gcggtggacg atgtgtgccg gaacgggggt
    32701 cggggaaact tgttgcatgt cgctacaata gtgaagcaga cggtttttcg caagaggcga
    32761 tgcgcgatga ccggagagtg ggggcggtgc gcagatctgt tcggtgaggg cgtggcgtca
    32821 accgtctgcc gctcgccgcg ttgaatcctg tgggtagagg aacatttacg ttgaggccat
    32881 ccacggcccg gacaaggatg gtgtcaccaa cagagaagga ggacagtatg cctactgggt
    32941 atccacgaaa ggcgacatcg atggtgctct ggagttgtgt cgtgatcgga ttgtctggtc
    33001 tgtatgcacc ggtgaacgcc gcaccggatc ctgcctccac gggtcaggaa cagccggcgg
    33061 cgacttccga agagcccggc agcagcggga agcgaccgga ggggcagggg cggggaggtc
    33121 gtccaggcag gaaggcttgt gccgatgacg tgaagaaatt gtgtgcgggg gtcaaagccg
    33181 gagaggggcg gatcatgcaa tgcctgagag agcacacgca agagctctct ccggcttgcg
    33241 ccgacatgat gcaacaacga ggaaagcatc ggccgtaacc cttgtatggc attgccgccg
    33301 ggcggcgcga tgagccgccc ggtcgaccgt tctcacggtt cacgcctctc cctctccatg
    33361 cctgtcgtgt gatttcgata ttttattctc gaagtgctgc gagtgcgata cagtaccctt
    33421 gatagatacg ggagggtacg tatgagtgat actactcgtc aggaacagtt ttcggtcgac
    33481 ttgcgtcagc acccgcgctt gcgtgtgccg gcgccgtttg cctgttcgtt atcccgattg
    33541 ggcctggcca agtggctcgg tcgcggaggc gaggggatcg gtgtcgtctt tgacgtatcg
    33601 atgcgagggg ccaaggtgat gagcgaagca ggaatccagc gcggcgatcg agtatcggtc
    33661 agtctgtcac ttcccaatca ggtctcgccg atgacggtcg agaaagccac ggtgcggtgg
    33721 gagcgggagc aggtctacgg cttggaattt gtcgacctgt cgccggtggc tgaaatgcgt
    33781 ttgcgaaagt tcatctccct ggcgacaaag ccggcagcct gaggcttcgg tgcggctcgc
    33841 gatttcttcc gcctggtaga gcagcctcga tacggtcgat ccttcgaact tggtgccacg
    33901 ttccagaaga cgggggtatc tgctcgaccc ctttcccctc tccttcgaca ttgcgctcct
    33961 cgctccgttt ccctacaatg actgacccgt ttggccatgt cgaaccctca ggcaaaccat
    34021 aagcggcgcc agccacgtag tgtctatcat ccgatgtatt ggcccacgtg ggtcggcatt
    34081 acgctctttc gactgctctc ctggcttccc tatcatgctc agcttgcgtt tgggcgtcag
    34141 tgtggacgcc tgttgcacgg gctgctcacg aagcgccggg acatcgtccg cgtgaatctc
    34201 gccctctgtt ttcccgaaca gtcgccggag gagcgcaata gggtggcgct gcactcgttc
    34261 gaatccatgg gaatgggtgt attggaaatc gccatggcct ggtgggcgaa agatccccgg
    34321 gcgcattgcg aatgcacgat caagggcttg gagcatatcc gcgacgcgct ccatcgcggg
    34381 cggggcgtgc tgctctgcgg ggcacatttg catgcggcgg aactagccgg tcgattcatg
    34441 gcgctggagc agccggtcgc catcgtgtat cggccgcaga atgatgtcgt ggccgatatg
    34501 gtcgcgaacc gctgccggag ccgatattat tcagaactga ttcaacatcg ggatatgcgg
    34561 ggcatccttc gcgcattagc caggaaccag gtggtctggt acgcgcccga catcgatgcc
    34621 ggatcgaagc gtagtgtgtt tgctccattc ttcggcgtgc cggcctcttc gctgacggcg
    34681 acgtctcgcc tggccaaggt cagtggtgcc gcggtcgtcc cctgctttta ttatcggcgg
    34741 gcggatgggt ccgggtatga catcgaggta ggggaagctc tggaccattt tccgtccggc
    34801 gaaatgatcg aggatgcgac ccgtgtcaac cggttgatcg aaggggctgt ccgtcgcgtg
    34861 cctgaacaat atttctggca gcatcgccgc ttcaaaagcc gtcctcccgg cgaaccgccg
    34921 ctgtatcggc gatagccgcc atccccggta cgcgggtttt tctcactgcc tcatcagtcg
    34981 ccgtggtcag cgaaatcatc agaaatattc ggcgtttcgc gccccctggg gtatactgac
    35041 gttggctgtt ttcagacgac gactgcacca tccgctccgc catggatcaa gataaacagg
    35101 tctctcccgt tcgcattgag ctccggcaac atcgtcgctg cccggtggcc gagacctgcc
    35161 tgctgtcatt tgcccccttc gcaccaacct tgagtttcaa tggcgatgcg gaaggtgaag
    35221 ggacggttct caatctgtcg cccggcggtt gcaaaatcag cagcgaggcc ggagtgaagg
    35281 tgggcgatgc catgtcgctg attattctcc ttcctggaga gactgctcct acggcggtcg
    35341 atctggcgtt ggtgcgatgg ggcaaggatt tgtcttttgg tgtggaattc gtctcgatgg
    35401 gcacagctga acaggatcga ttgcgccaat ttctcgcctc cgccgctgtc tcggagtaag
    35461 gtgataccga cgtggatatg gtgcgcgtga cgaagaggag tgcaggtgga agaaacaaga
    35521 accttgcgta agtcccggcg cgtcgcggcg gattgtcgtc tcgagttcgt cggcgaagat
    35581 gagattgaag gagaggccga ggtgctggac ctctcgtcga ccggatgcat ggcaaaatcc
    35641 gaccaggcga tgccgccggg ccttcgcatg aaagtctcct tattgttgcc cgacggacat
    35701 gaatggccgg ttcgcataga ggaagccgtg gtccggtggg cccggggcgg agagttcggg
    35761 gtggaattcc tggtgatgcg gcctccgcaa ctccagcgca tccagcagtt cgtgatcaag
    35821 catagacccc tgggttaagt cgcgatcgac acctgaccct ctctgtccct tgcccggttg
    35881 ccccccagcg attcgtgcct ttccgccctg agcctgccac tcccgtttcc ccactcgttg
    35941 ctttacgatg actgcttccc tacaatttcg ccgtcgatcg cggcttcagg cgcccgacat
    36001 gctccttaca cgcgaggcga ttcagaagac atgttcacgt gggtcggacg gaagaccatc
    36061 gcggggtacg cctatctggc ggcgttggcg acgctgtttg cgcaagccgt ttttgatctg
    36121 atcattcccc cgaagcaggg gcggggggag accttccggg tgttggtcag gcagattctt
    36181 ttcacgggag tcgacgccct gccggtcacg acagtcatcg ccctgttgct cggcatcatc
    36241 attgtgactc aggccggtac gcagcttccc aggctcgggg caggggggct ggtgggcagc
    36301 atcatcgtgg tcaccgtgat ccgagaactc ggccccctgg tcacagcatt tattgtggtt
    36361 gggcgttccg ggtcggccat tgccactgag ctcgggaaca tgtcggtgac acgcgaggtg
    36421 gtcgccttac ggctcatggg gatccccatc agccggtttg tcatcatgcc tcgtatggtc
    36481 ggcatggtgc tctcgatggt gtgcctgaca ctgtatttcg atgtggtggc ggtcttcggg
    36541 ggctacctcg tggctgatgc gcaattgacc attccgctcg atgcattcgc ggagagtgtc
    36601 atcagggcct tgtccacgac ggacgtcctt atgacggcga tcaagggcct gggctttggc
    36661 tcggcggttg cggcggtctg ttgttaccat ggcctggctg tccgatcgtc ctatacggag
    36721 gtgccgcaac aaacaacccg cgcgatgatc aactctttcg tcctgtgtct gctgatcgat
    36781 gtcgtcgtga cggtaccgtt gtatctatga gcgaggcagc cattattttc gacaaaatcc
    36841 gcgcgcccat gttgggcgga gacctatggc tggaactcga tctcaaaatt atggcggggg
    36901 agtgcgtcgt ctgtgtgggg ccgagtcgtg ccgggaagag tttgatggtg gagcttgccg
    36961 ccggactgat tgctccggaa tcggggcggg ttcttgtgtt gggccagggt tggagcgagg
    37021 tttcggacga tgctccgggg tccgtacggc tcaaggtcgg agtggtgctc caacagccgg
    37081 ggttgttgag caatatgacg ctctttaaca atgtgctgct gccgctgcgc tatcatcgaa
    37141 gtgccatgcc ggatcgtcac cgtgaacaag cggtgatggg agagttggag cgattaggtc
    37201 tggcctcgct gcgtgatcgg tttcctgcgg agctgaatca gggggaggcc cggcggggag
    37261 ccattgcccg agccctgata ttggagccgg aggtgctcct cctcgatgat cccgtcgccg
    37321 gcttagacgc tgacatggtg ttgggactca agagctctat cgacgaacgg cgggcccagc
    37381 ggcccctcac ggtcctcgcg gcgttgcgca cctgctcgcc attcgttgat gccgccgatc
    37441 gcttgattgt tcttcgcgat ggccatgtgg aagcggacgg aacccgtgac acggtgcgtg
    37501 acctggcccc cgaagcgttg cggaggtatg tagaatagcc gtgcgatatg gtcggataga
    37561 gaggtgagtg atgatgcact attcgcatcg tctgtctagc gggcggctgg cgcagatcgt
    37621 gggcacgttt gtgctgatcc ccgcggtgat tcttgccgtg gctggttttt ggatgtcgag
    37681 gacggaacac ctgttcgagt caaaatatct gctcaaggcc agcttgagta agtcttacgg
    37741 cctcgagccg ggggcgccca tcgtcgtatc cgggattcgc atcgggcgcg tcaagcaggt
    37801 ggacctcaat gatcgaggga ccgtcgatct gacgcttcag ctcttggcgc ggtatcagga
    37861 tatggtgaag gagaattccg agctgcacat caccaagagt ggtgtggtgg tgggccagac
    37921 gcaagtcgac atcgccatgg gcacggccag cagcccggta ttgtccgagg gctccatcat
    37981 ccgtgccgtc gaaccgcggg acatcggaga cttgctcaat gaggtcgagc cggtactgat
    38041 ggccgtgaag caaacgctgc tgcgggtaga aaccatcacg caggatatgc agggtggcct
    38101 gaaggccggt gggaaagcgc ttgaacaggt tgccctggca acgcaggagc tgccggcagt
    38161 cgtcgcctcg gtacagcgga cgattgcctc cgtggaacag acggccacgg cgctgcccgc
    38221 catgaccgga tccattaagc ggacgctgag catggtcgat cgcgtcacgg ccgatgtcca
    38281 acagacgacc ggacggcttc cggccattct cgacagcgcc caaacgacgc tcaccagcgt
    38341 caagcgactc tccgattccg tgagtgaagt gagtcaggag ctgggcccca tcatccacag
    38401 cgccgagacc acgttggcgg atatgtcgat tctggtgcgt ggagccaagc agacatttcc
    38461 tttcaatcgg tttgtgcaga atgccgggcc ggcgcccaag gagtcaccgc caccctcgcg
    38521 ggaagggact caaagtttga gaggagatca gttgcgtcga tgagagtgcc cacaccagct
    38581 tcaggagcca catgctgcgc ggtggcattg tgtctgctgt cgggatgcgc gagccccccg
    38641 ccgcccccct ctcctgcgcc actgcccaga ccattggccg agcagggtga atcgttgcgg
    38701 cgtcaaggtg accgttcgtt catgcgaggt gatctgactc gcgccgctga agagtatgag
    38761 gcggcgcgcc ggatggcgga aagcctggat gatacgcagg ggcttatcgt cgcgatcaat
    38821 gatgtcgggc gcgtggctct caggcgcggg gacgttcccc gggcggtcag ttcgcatgag
    38881 cgagctgtgg ccttggcgca acaacgggga gagccggatc ttctgattgc cagtctgact
    38941 gacttggcca ctgcggaaca tcaatcgggt gcactccagg aggcagaaca tcgatacgaa
    39001 caagcgttag acctggctca gcagcgaggc gatcgttcgg ctgaagcgac aatccgtaac
    39061 aatctcgggt tgatacggca gaccaagggc gacgtggagc aggccgcgca actgttccgt
    39121 caggccctgg ccttgaatca agcactggga aatttggaat ccgaggccag caaccatgtc
    39181 aacctcggca tgttggccga agcacggcat gagtatgacg cggccgaacg ggaatatgag
    39241 cgagccctgg agttagacaa aacggcggag cagcggatgg agattgcggc ggatctcttg
    39301 cggctgggga agattgcgga tcgacgagga tttcccgatc gaggccagac gtattcggag
    39361 cgggcttacc aaagctacct ggcacaagat gcatttgccg aggcccggtc ggcgctgagt
    39421 caggctgtgg cttgcgcgaa gaaactggag cgggccggag acattgcgcg gttggaacgg
    39481 gaattgaccc gtctgaccgc ggtgcatccc gcacggtagc cggttcccct ggcgtaccgc
    39541 cgcatgctta cttgatggcg acggctttga cttctttata cgtcgtgtgt ccctgcagga
    39601 ctttctgaat gccatcctgc accagcgtcg tcatcccgtc ttccatgccg gcatgcagca
    39661 tctcatcggt cttcgcttta ttctgaatca acttcttcat cttgtcggta cccaagagta
    39721 actcatgcaa cgccacgcgg cctttcaggc cggtgcgatt gcaggcgtcg catcctttgc
    39781 cgcgatagag acggaagttc gcgtcgaacg gcactttgag ggcgtcccaa tagtcagggc
    39841 cgtaccccaa acgcagctcc tcatactctt ccttcgaagg ctgataggcc tccttgcagt
    39901 ctttgcagat gcgccgggcc aaccgttgag ccagtacgcc cagcatggcg tcggcgaaac
    39961 tgaatgagtc gcagcccatg tccagcaatc gggtgatggt ttccaccgcg ctattggtgt
    40021 gcaacgtgct gagcaccaag tgacctgtca atgaggcttc aatgccggtg tctgccgttt
    40081 ctttatcccg catttcgccg accataatga cgtccggatc ggcgcgaagg aaggcgcgca
    40141 tagccgccgc gaaggtgaat ccgattttgg catgcacctg gacctggcgc aggccgtact
    40201 gcgtgatttc gaccgggtct tctgcggtcc agatcttgat gtccggcgta ttgatgtagc
    40261 cgagtactga gtgcaacgtg gtcgtctttc cggaaccggt gggacccacg cagagaatga
    40321 tgccgtaggg tttctcggcg atgcttttga tctcgcgcag attacgttcc gaaaagccca
    40381 ttttatcgac cggcagcggc tcgctggcgg ccaggatacg catgacgacg tcttcgttat
    40441 agccggcagt cgggatggtg gcgacgcgca gctcgatttc cttgttttcg ccgattttaa
    40501 atttgatctt tccgtcctgc ggcttacgcc gctccgcgat gtcgaggctg gccatgatct
    40561 tcaggcgcga cacgatggca cggcgatagc tcggtggaat tttcatgtac tcgaagcagt
    40621 cgccgtcgac ccggaaccgg acgagggtgt cgcgtttttc accgtagggt tcgatgtgaa
    40681 tgtcggaggt gccttgccgg aacgcatcgg caatgatttg gttcgccagg cggacgatgg
    40741 cattatcgtt ttcgtcgagt cccgcggcgg aggcttcttc ctgggcttcg agctgggatt
    40801 cgttgaccag ttcgccgaga atgtcggaca cattctcgtt catcttgttg gtgacgtcgg
    40861 gggttccggt cgtgcttgcc aggaagagcg agatgtcgcg gcgcagactg acggcaaacc
    40921 ggatgttgag ccccgggaag gtgcgcttga tgtcttgaac gcggtcgaga tcgccgggat
    40981 cgtccgtgag aatctcaatg gcggcgcgat cccgcttgag cggcatccag tgattcttct
    41041 tcaggtagtc gacgttgagg ttcttgagca attcgatgtc gacgatcgtg cgctcgttgt
    41101 attcgatata ggggcacttg tggaaattgg cgagtgattt gccgatctca gctttcggga
    41161 tcttgtattt ctcaatgagc agcgactcga tatccgtcgt gccctttttt gcctcagcga
    41221 gtgcgacatc gagatcgtgt tgggaaattt ttccattggt gagcaggtag tcgtatttgg
    41281 acggcggctt tttcgaggtc tttcgaaggg tgtagaacgc ggtgcccagg gatttggcaa
    41341 tctccgcgac gcactcttcg tctttgcgcg tgaagcgtcc gccgctcttt ttgttcagga
    41401 gttgaatcac ccccatcaga tacttgtttt cggcgacgac cggatacgcg agaacttgtt
    41461 tggttttgaa gccggtactt ttatcgtacg tggcatcgtg ggtcagcgac ggatgaatcg
    41521 cgctcagttc gaccaagctg taggcatcac cgacattgac cgggcgcagg tatttggcgc
    41581 aaaaccccgc caggctttgc tcagtgatcg gcgtacgcac ttcctggacg gtatcgagat
    41641 gaggaatctt ggagaagatt tcttttttct cggaatccac gacgaagagg gtgaggtctt
    41701 ctgcgtcgaa acatccgagg atttcgtggc gtagatccag gagaatttga tcgacgtctg
    41761 cggcggcgag gatttgtccg ctgatccgct tgacctgttc ggcgttcccg gatttgagag
    41821 tggcatcgcc actaggcagc ggagctttag gctgcatcaa cgtgtctcca cgcgggctga
    41881 acggggaatt gcatgcaggg tcagaaacgc gatgccggcc tggaatcgtt cgccgtacag
    41941 gagtttcgga cggtgcgtct tccgtgtgag tcagtgtatc gcaacttgta gtcaaggcaa
    42001 gcaaaaggca ttttctcaaa ccccggcctg gagcttgcct tcgtcggcgt gatgagattg
    42061 tcgtgaaggt ggcgaacgag gatcgggagt ggattttagg acgcagcttg gagcagtgcc
    42121 gtgagtgtcg gaatgacctg atccggagca atttttttcg tttcgccggt gtgacgaagt
    42181 ttgagctcga cctgcccctg cgccaagcct ttttctccga tcaccaggtg aaagggggcg
    42241 ccgatcaggt cggcatcgtt gaatttaaca ccggctcgct cgtcgcgatc atcccacagg
    42301 acttcaatgc cggcttgttc catcgctcgg tagagcgact cggcgagttg gagaaccggc
    42361 tcagactggc tgagcggcaa cagcgtgaca tgaaagggcg cgataggaat cggccacttg
    42421 atgcccttgg cgtcgtgatt ctgttcgact gatgcggccg aaacacggct cacgccaatg
    42481 ccataacaac ccatgacggc cagttgttcc tggccctgcg catccaggaa ggtggccttc
    42541 atcgcctggc tgtatttcgt gcccagcatg aagacgtggc cgacttcgat acccttcgcc
    42601 gtcttgagca cgccatcctt gcgcggggag agatcgccgg cttgcgcatt gcggaggtcc
    42661 gagaattggg cgactgtgaa atcgcgttcc cagttcacgt cggtgaaatg ggtatcgacc
    42721 tggttgccgc cgacgacaaa gttcgccatg gcctttacgg accaatcggc gaggattcgg
    42781 atctgtttca agccgactgg ccctgcaaag ccgactggtg caccggtcag cttgggtatc
    42841 aactccggcg cgacgagctc gatatcagtt gccccgagca ggcgcttgag cttgatttcg
    42901 ttcacctcat gatcgcctcg aaccaggacg gcgacggttt ccttgccggt ggtgtagagc
    42961 agcgttttga cgagttgcga gggagcaatg gttaagaact tggtgacctc ttctaccgtc
    43021 cgtcgccccg gggtggagac ggcggtgagg ggtcgtaagg ccggcagcgg ggatacgtcg
    43081 ggtggaagga cctcggcccg ttcgacgttg gcggcgtagg tgcctccctc ggcgtagacg
    43141 atagtttcct caccggtttc tgccagcacc ataaactcgt gggaggaact tcctccgatc
    43201 aatccggtgt ctgcttccac gggacggaag gtcaatccgc agcgagcgaa gatgcggtta
    43261 taggcgtcat acattttctg atagttcagc ctggctcccg cttcgtcccg atcaaagctg
    43321 tacgcatcct tcatgatgaa ctcgcggccg cgcatcaagc cgaaacgcgg acggatttcg
    43381 tcacggaact tcgtctggat ctgatagaag ttcagtggca tctgccgata ggaacggact
    43441 tcgcggcgga agagatcagt aatgacttcc tcgtgggtcg ggcccaggca gaaatcgcgc
    43501 tcatgccggt ctttgaaccg gaggagctct ttgccataga agtcccaccg gccggtttca
    43561 cgccacagtt cggccggcga tgcgaccggc atgaggactt cctgtgcgcc ggcccggttc
    43621 atttcctcgc ggacgatgcg ttcgattttc cgtagaacgc gcaacccaag cggaagatag
    43681 gtatagatgc cggcggcaac tttccggatg aggccggcgc gcagcatgag gcgatgactg
    43741 accgtttcag cctcgcccgg atcctcacgt aatgttggaa tgagagtttc ggagacgcgc
    43801 atgtggggct atggggaaat caatcgctcg atgtcattcc aaaaggcgaa aatcataata
    43861 ccgaccagaa gtaccaaccc cacctgttgt gccagttctc gctgccgatc ttcaagtggt
    43921 tttcgtcgaa tcgcttcaat aaaaaagaat aacagatggc cgccgtcgag aattgggatg
    43981 ggtagaagat tgaggacacc caggttgatg ctcagcatcg ccatgaggaa gaccagactg
    44041 gaggtgccct gttcggccgc atcgccggcg gttttggcga tggtgagcgg gccgccgata
    44101 ttcttgcggg aaatatctcc ggtgatgatc ttgtaaattc cgaccaccgt gagttcggtc
    44161 catccccacg tcgcctgggc gcccaataac ggggctttca agggttcgtt ggtttgtaaa
    44221 atagtctggt tctgggcaga gatgccgatt tttccgatct cggccggctt tccgtctacc
    44281 gtggcctttt cacccatcgg cgttacggaa accgattggg cgcttccgtt tcgttggatc
    44341 tcaaactgca aggcgtgatt cggattttcc cgcaccagcg cggtcatctg cgaccaggtg
    44401 aagatgtcat gcccatcaat gcgaaggaca cggtcgccgg ctacaagccc agcggcttgt
    44461 gcgcgcgatc ccgggatgac ggccgtaata accggcgcgc gttcctcaat cccgagctgg
    44521 aacagggtcg tggctttgcc gttgtcttgg acggtggtct tattgggggt gacgagcacc
    44581 gttttgatct gctgcccgcg cgtcaggtcc aaggtgagtt gcttcccatt gctctgggcg
    44641 atgtacttca gcagttccgc attcgtcgaa atttctttct cattcacccg gatgacccgg
    44701 tcacctggtt tcaatcctgc ctgatcggct ggcgaccccg ggagcacggc ttcaatttca
    44761 ggaatgatgt ctttgaagct tggcacggga agggtgtagc caagcccgat gtaggccgta
    44821 tagatgagat aggccaggat gaagttgaaa atcggtcccg ccgccacaat cagggtcttc
    44881 ccccagagag attgatgaac gaaggcccgc tttttgtctt ccggagtcag cggttcatgc
    44941 tcgtcttctc cgaacagctt gacgtagccg ccgagcggca cgatcgacag gaggtattcg
    45001 gtttcaccga tctgtcgtcc gaagatcttg ggcccgaacc cgagtgaaaa cttcagaacc
    45061 ttcacgccga cccagcgtgc cgctaggaaa tgccccgctt catggaaggc gacgagtacg
    45121 cccaggacca ccaggaacgg aagggcccag tgagtcagca tcgacaccac gtcaggggac
    45181 caggcaaagg ttgttcccac aacaacactc cttaggttca gggtattacc gcgtcaaggc
    45241 atgcacgagg gactcggctt tttcccgagc ccagcgatcc gcttccagcg catcatccaa
    45301 accatcgatg ggtctcggct ggtgagcgtc cattgtactc cgaataatgt ccggaatatc
    45361 gagaaaacga ataccgttct gcaggaaggc ttccaccgcc acttcattgg cggcgttcat
    45421 cgtggccggc atactgccgc cgatccgcag ggcgtcgtag cccagttgca ggcacggaaa
    45481 gcgatcatga tccggcttga agaaggtcaa ggtgctcact tcggtcaaat ccagagaggg
    45541 aagatccaac ggcatccgtt caggataccg catggcgtaa gaaatagggg ttcgcatatc
    45601 gggcaggccc aattgcgcga tcatcgaccg atctctgtat tctaccagag aatggataat
    45661 gctctcccga tgcaccaaga cctcgatctg ggtttccgga atatcaaaga gccaacgcgc
    45721 ctcaataacc tccaatccct tattcattaa ggttgccgaa tcgatggtga ttttcgatcc
    45781 cattttccag ttcgggtgtt gcaacgctcg ttcagggctg acgtcttgca actgctcccg
    45841 ggaaaagccc cagagcggtc cgccggatgc cgtgaggata atgcgtttga cgtcctcacg
    45901 ccggtgccct tcaagggatt ggaagatcgc gctatgttca ctatcgatgg ggaaaatgcg
    45961 aacctgatgt ttgtgggctt ctgcctgcat caaggcgccg gccatcacca tcggttcctt
    46021 attcgccagg gcgatttgtt tgccggcctt gattgcagcc agcgtgggca gcaatcccgc
    46081 gccgcccacg atcgctgaaa tcaccaattc agcgtccggg gattgtgcga cctgggccac
    46141 gccttcgttg ccggagagaa tttttaccgg caggtctgcg caccgttgtc gcaacttcgc
    46201 ggcggccggt tcgctggcca gggcggcaaa ggccggccgg aaacgccgga tttgcgcttc
    46261 aagtttctcg tcattcgagc cagcggtcag accgatgacg cgaaactctt ggggaaaccg
    46321 ttccacgata tcgagggtgt tcgtgccgat cgagccggtc gaacccaaaa tcacgatgtt
    46381 cttcatggca tactccagag caggcgaccg atcacccgtt acacggtcgt gacgatcgcg
    46441 acatagtagt agaaggccgg tccagtgaac aagaggctat ccagacggtc cagcatcccg
    46501 ccgtggcctg gaatcagggc gccggaatcc ttgaatcccg tgctgcgttt catggccgat
    46561 tcagccaggt cgccgatgag gccggcgagg gtgaggatca cgccaagcac cagacaatcg
    46621 acgagtgaga tgctgggaag gaaccaggcg cgtgccgcga ccgctcccag gcaggccaga
    46681 agaatgcctc ccgccaagcc ctcataggtt tttttcgggc tgaccaccgg ggccaatggg
    46741 tgtcgtccaa cggactttcc tgcgatgtag gcgccggtat ccgctgccca ggtcaccagc
    46801 acgacaaaga aaatcagtaa ggccccgtca ggcatggctc gaatgagcag caggtagccc
    46861 agcaaaagtc cgatatacag gacgcccatc accagcacca tgccatcgtt cagcgaatcg
    46921 cgcagcgctt tgcctgagag caggggcata cagagcgcca tcaccacggt cgcgatcaag
    46981 accgtacggt ctgccacgat tgtcggccac tggatgctgc tcaacagtaa ccccgttgcg
    47041 acgactcctg cccaactcca ccagggccaa ggcgcttggg ccaaatgaag ccgatagaat
    47101 tctgcgaccg ccagcatgcc ggagagggca accaatccga agaaggcgat cggcccgagg
    47161 tcgtgcacca ggatatagaa aatcggcaga aaaacaaccg cggccatcac ccggcgaacg
    47221 gtttccgagg agctgcgtgg tcgagccttg tccaccgtgg gtgacatctc ctcgcactgg
    47281 catgctcccg gtttcaccac gggaagacta ggaggagacg ctactgaaca ctcggccgaa
    47341 acggcgctcg cgccgctggt attcgatcaa cgcgaccaag gtttcgcgcc gacggaaatc
    47401 cggccagaga gtcggggtga agtagagttc cgtataggcc agttgccata acagaaaatt
    47461 gctgatgcgg gtttcgccgc tcgtgcggat caacagatcc gggtcgggca ggtcgtgcgt
    47521 atacagggcc tgttgcatgg cgcgttcatc gatctgctcc aaagacaggc gcccctcctg
    47581 cacagaccgc gcaagctcct tcgcggcatc ggccagttcc gcgcgcccgc cgtagctgag
    47641 cgcgacgttc aggattaact tgtccagatg cgccgtttcc tgttcggtcg tgcgcaccca
    47701 gcgcagtgcc gatggcggca gcgcagtgac ccggccgatg gtctggaagc gcacgccctt
    47761 ttcgaccagg ctggaacgct cggtggagag atagtgttcc agcaatccca tcaaggccgt
    47821 gatttcctgg gccggccgat tccaattttc ctgggaaaag gcatagatgg tgagcacctt
    47881 gatgccgagg tcgagcgaca gcgagatcaa ttcccggacg gacttaatgc cttcctggtg
    47941 accggcaata cggggtagtc cacgcaattc ggcccatcgg ccattgccat ccatgatgac
    48001 ggccaaatgt ttcggcagca gatcagggtc gagttgcgag agcaggtcgg agtcgggtgc
    48061 aggctcgata tcgcgggatc gaggatcgct catggaacgg cgtaggcacc ttttcttgtg
    48121 acgacgtgaa tccttagaca ggtaacgcga gtctagcggt ggggtgtggg aaagtcaaac
    48181 tatatttccc aatttcgctg tatttgcagg tggttcggca gtgtttacat tgcgcctcca
    48241 gaaaagagta cgttatactc acgcaccgta tgagaggagt aatccatgac caataacgag
    48301 accggcgcgc tggcgcgttt cggggtcacc gatcgagata tcgagcaggc gctgggacgg
    48361 gtgaaggtct ccgctgtcga ctatgccgac ctgtatttcg aatattgcca gtcggaatcg
    48421 gtctccatgg aagaggggat cgtcaaacgc gcgacaaaaa gtgtcgggca gggtgttggg
    48481 gtgcgggcga cggccggtga aaaaaccggg tttgcctatt ccgatgaact caccgccaag
    48541 gatttgaaca ttgccgccga tacggcacgc tatatcgcca attctgtgca aggcacggcc
    48601 ccggttcccg tcacccatcg gccacctctg gctcgtaatc tgtatcctca cgaccgcgct
    48661 acgatcgaag tggcgacccg ggaccgggtg acgctgttga acgccatcga tgcggaagcc
    48721 aggcgctatg atccccggat caagaacgtc atggcggcct ataacaccga atataaagtg
    48781 atgttagtcg cgacgtccga cggactcatg gtcggagata ttcagccgtt gtccaggcta
    48841 caggtgacct gtatcgcgga agaaaacggt aaccggcagg tcggtacatt cggcgggggc
    48901 ggccggatcg ggctggaata ttttcaaaac gatcagcggt atctgcactt tgcgcgggaa
    48961 gcggcgcggg aagccattct gaatctgagc gccgtcgatg ccccggccgg tgtcatgccg
    49021 gttgtgctgg gcggtggttg gccgggcatt ttgctccacg aagccatcgg ccacgggttg
    49081 gaagcggatt tcaatcgtaa gaaaacctca gcgttctcga ccttgatggg gaaaaaggtg
    49141 gcgtccgagg tctgcaccat cgtcgatgac ggtacgcttc cgttccggcg cggttcgttg
    49201 aatgtcgacg atgaagggac gccaaccagc cgtacggtct tgatcgaaaa gggcattcta
    49261 cgtggttata tcaccgacaa actgaatgcc aggctcatgg gcattccggt gaccggcaac
    49321 gggcggcgcg aaagttatca gagtgtggtg ttgccgcgga tgacgaatac cttcatgctg
    49381 gccggtgaat ccgatccgca ggacatcatc aaatcggtca agaagggcct ctacgccgtg
    49441 tcatttggcg gcgggcaggt tgatatcacc aatggcaaat tcgtgttttc cgccagcgag
    49501 gcctacctca tcgaggacgg gaaaatcacc cgtcccgtga agggcgccac gttgatcggc
    49561 agcggaccgg acattttgaa gcaagtttcc atggtgggcc atgatttgaa gctcgatgaa
    49621 ggaattggca cctgtggtaa ggaagggcag tcagtcccgg ttggcgtcgg cctacccacc
    49681 atccgcatcg acgaaatcac cgtcggtgga acgaaagcat catcatgaca cgtatgatcg
    49741 gagagcaaga ctatacgacg gtcgcgcagg atttgctgac gcgagcttcg aaacagggcg
    49801 cgacggcggc ggacgtcatg gtggccgacg gggaaaccct ctcggtacag gtccgaatgg
    49861 gggccgtcga tcgcttgacg aaagcgcgtg agaagcgatt ggggttgcgg gttttctttg
    49921 gccagcggtc agccagtgcg tccacctccg acttttctcg cgactcgctg gaacggtttg
    49981 tccaggaaac ctgtgccttg gctcaagcgg tggtggagga tcctgtctcc ggccttcccg
    50041 aaccggggca gttcgctacc gaattgcccg agttgcacat ccacgatagc acgaagctgc
    50101 agacggatga gcagattgcc ttggcccttc gcgctgaacg cgcggcgttt gccgcggatc
    50161 ctcgcattac gaactcggaa gggggcgaat gcgactcgtc ctccggccgg atcattttag
    50221 ccaacagcca tgggtttctc gggcactacg ccaacagcag tttttccctg tcggtctcgc
    50281 cgatcgcctc ggatgcggcc gggatgcagc gggattattg gtatggcgtg aaccgatcgt
    50341 ttgccaaact ggaaagcccg gaagccatcg gacaagaggc caccaggcgc acggtccgca
    50401 agttgggcgc ccgcaaggcg gcgactggcc gggtgtcggt gatctttgat cccgaagtgg
    50461 cgggcagtct gctgagtcat ctctgcagtg cgctgtctgg ttacgccctg tacaaggggg
    50521 cctcatttct catcgggcaa ctcggccagc agatcgctcc ggactttgtg accatttatg
    50581 acgacggccg gatgcctggc ggtctgggca cgcgcccgtt tgacggcgag ggattgccga
    50641 caagaaaaca ggccgtggtt gaacgcggcc ggttgaccag ttatttgctg gacacgtact
    50701 ccgggaaaaa gttgggattg ccttcgaccg gaaacgcggc gcgcagcatc ggcgaaagtc
    50761 cctctgccgg cccgacgaac ttttatatgg cgccgggcac gaccaggccg gaagacattc
    50821 tggcgtcggt gaaacaagga ttgtacgtga ccgatctgat cggcttcggc atcaacatgg
    50881 tcaccggaga ttattcccgc ggggcgagcg ggttctggat cgagaacggc gagttagcct
    50941 atccggtcga agaaattacc atcgcgagca atttgaagca gatgtattca gccattgaga
    51001 caatcggctc ggatctggtg ttccgcggac gcatcgccag cccgacggtg aagattgccg
    51061 aaatgatggt cgcagggaac tagcgacaaa ggggggatgg gtgatgcaac gattgatggc
    51121 ctcaatgctc ggcgccctac tgacgatccc tgcgatcggc atggcggcag agtttcacgt
    51181 gaccagcccg acgatcaagg atcagagcac gatcggtagt gaacacgtgt tcaacggatt
    51241 tggctgtacg gggcagaatg tctcgcctgc gctgcaatgg gagcatgccc cgaaggacac
    51301 gaagagtttt gctgtgacgg tgtacgatcc cgatgctccc accggaagcg gctggtggca
    51361 ttggctcatc ttcaaccttc cgccgggtac caccggtttg tcggctggtg caggaaatcc
    51421 ggacagcgcc ggcgctccgg cgggcagcat tcaaagcatg accgattttg gacagccggg
    51481 cttcgggggg ccatgccctc ccccgggaga caagccgcat cggtatatct ttacggtctt
    51541 tgcgttgaag gtggaccaat tgccgctgaa gaaagacgcg tccggcgcga tggtcgggta
    51601 ttacctcaat cagaatgccc tcggaaaagc atcggtcacc gggttatatg gacgataagc
    51661 aggcaatcat cagaacagag taaggaacac accatggctc ttgatgccga acagggaaaa
    51721 atcatgctgc agctgatcac ctcgcgatac gaggatcggc attggcgcaa gaagatcgag
    51781 aagacgctca gtttgccgca gagcggagtc ggggacgagc gccagcagca gatgtttctc
    51841 tatctgaagc tgaatttgaa ggcgtacaaa tctaggcggg cggaccccga cgactggatc
    51901 ttaggcggat acgccacgaa ggaagtcatc gatcgggcga agtttcaacc gcacttggtg
    51961 gggcagggaa tcacggctga ggacgtcgcg ttcctcggtg ttgatccggg cccggatgtt
    52021 gatgaagcct ggtgggaaga catgctggtt gcctggtttg gtgctcccga agatgaagaa
    52081 gcgactgagg caacggagga ggaatctccg tccgaaaccg ctgccgagag tcatcctgcc
    52141 cgtacgtgaa cgaacgcgct gtctggctgc ttcgcctgtt ggtgaggcgg ccaggccagg
    52201 cggcgttgct aagatccgaa taaatccatt tgctgggcgg gcgaagacgg acgctcgatc
    52261 tcaagtgtcc gtgcagcact ggcaacttct tgttccttcg ccggggctgg ctgattgtgc
    52321 cggtccaaaa actccttgag cttctgaaaa tcttcccgca agggctgcag catgctgctt
    52381 tgatgcaggg ctgcggcagg atgaagcaaa ggaaagagca caaagtcttt catgtaaaaa
    52441 gcctgcccgc ggactcgcgt aatgcctacc ttacgttcca gcagcgtttg tgtcgcccag
    52501 ttgccgagcg agcagaccaa cttcggtcga atcagagcga tctgtttcag gaggaacggt
    52561 ttgcaggtgt cgacttccgg cggttccggg tcgcgattat tgggcggccg acatttgatg
    52621 acgttggcaa tgtagatatc tgcgcgggat agtccgaccg actgcagcag ttcattcagc
    52681 aattgccccg ccgcgcctac gaacggctca cctttttgat cctcgtgaaa gcccggtgct
    52741 tcccccacaa acatgacgga ggcctgcgga ttgcccacgc cgaataccac ctgacgccgg
    52801 cctagtttgg cgagcgggca tcgttggcag tcgtgaagcg agtcggcgag ttcctgcagg
    52861 gcgatatgcg gtgaagacat ggacgtgaca tctcgcgtgg agcgccaaat cttagtcgcg
    52921 ccatcggggc ttgtcaacgc tgcgctgcgc gttggcgcgc accggtgcga gtcgttatga
    52981 gggacgggta gagatggcct cggcacaacg gtggccgctg agaatggctg attccagatt
    53041 ggccggccat cccgtgtccg tccacgctcc cgccacgaaa aaattggtaa aggggctggt
    53101 cgagatcggt cgatactgat gcgtgcccgg tttgctggcc agcatggcgg aggcgaggcg
    53161 catgacgtcg aagtcgagaa tgcgcggcgg cgacgtcgtc gggaatgcgg ccgccatggc
    53221 atccgcggca aggcgaacga gttcttccgt cgactgtggc aacagactcg gctcgcccac
    53281 cgcctgagcc cagaggaccg acgtctgctc gtgtcgctct tcatcgggat gacggatgat
    53341 ccagtggaaa gtttttcctt cgaggaggat gagctgtgtc tgtcgaaccg gctggtcgag
    53401 gtgcaggcga accatcacga gcggggattc gctgagacgg ctgagttgct ggaaataggc
    53461 atagtgagtc accacgcgtt ccgggagaag cggtgtgaga cgctggggcg gcagcgcgga
    53521 gagataccag tcggcagtga gcctggtccg atcggctaac tcaacttctg tgactcggtt
    53581 gtgtgagaag cgcacttgag tggctgtggc attcagtttc actcgaacgc cgagccggtc
    53641 gagttctctc gtcaggggcg ccagcaaaaa tgaatccact ccttgaggcg gcacgatcag
    53701 cttcgttgcc cgggcaccgg tcaagaaaca ccgccgcaag gtacgcataa acaggccggc
    53761 agaaatgtct tccagcgcgg ctcccaacaa cagcctggcg agactgttcc acacaccgtg
    53821 acgggcggtt tccgactggc cgatgctcgt gagccattcg tcggccacac gcgagtcgag
    53881 atcgttcggc agcggcgggt cctgttccca ggtccgctcg agaaacgaga gcaggtgcca
    53941 gcggtcacgc atggacaggc cctggaataa ggtcgtcccg agcaacgtat ttaatggaga
    54001 ggggagagga agatgcagga actggatgcg ggcgccggtc gattgcaaaa attccaacgg
    54061 agtatgccgg agcggtcggg tcactgcgtc ctggccgaga gtcttgagca atgaccaggt
    54121 ggctccatgc gcttcgagga gaatgggtgg cggcgcatgc cacatccggc ctccaaggtg
    54181 agcgccttgc tcgatcacgg tgacggcgtg gcctttggcg gcgagggaac gggccgcggt
    54241 cagaccggcg atgccgccgc ccaggatgag caccgatcgg ctcatggggc ggaggagaga
    54301 aggcgggagc gcagccagac tccggctgcc accgctaacc ggtggctagg ggtgagggtc
    54361 acccgatctc cgagcacgcg gtaaccggat tgctcgatgc gctgcaaaat acggctgtac
    54421 acaccgcgca tgatttcggc tacggtcaga gcctggcgtt cttcgcgtgg cagtgactcg
    54481 agcgcgcgcg cggctttggc ataaaattcc ttcgcgcgtc cgacctcata gatcatgagt
    54541 tcggtgaact ccggcttata ccggcggtgc agaagatcgt cttcccggta gccgaagcgc
    54601 gtgaaatcct cctgcggcag atacacgcgt ccgcactcgg cgtcgttgcc caggtcgcgc
    54661 agaatgttgg tcagttgaaa ggccatgccg agattcaccg cataatcctg ggcgcgcgga
    54721 gacgtagtgc caaacacgtg caggcagatc aaacccacga ccgaggctac gcgataacaa
    54781 tagagcgaga gttgctcgaa cgtggcatat cgggtggtcg tgaggtccat ctcgacgccc
    54841 ttgatcaatt cttcgaaata cgcctgcgga atggacaatt cgcgcacgtg tcgggcgagg
    54901 ctgatggtca ccggcaacgt cggcgtcccc tcataggctg cgctcagttc gcggcgccag
    54961 cgagccaact cctcttgcgg atggctaccg gggggtggtt cgtcgacggc attgtccacc
    55021 tccttgcaga aggcatagac ggtgtacatg gcgtcgcgac gcgcctttgg caggaagaga
    55081 aaagaatagt aaaaattgct gccgctcttt ttggtcaggg tggtgcaata ggtttgcgct
    55141 tcggtcggtg tcatcatcgt gtcattgacg tcggaatgtc atcggaggtg agttgctcga
    55201 cggcctcggg cagaccatga ttgaaacaag ccgggtggaa gatggccgcc agctgttcca
    55261 cgccatcgat gagtctcggg cctggtcgac tgaagtacga gagcgcatcg accagataca
    55321 cctgtccccg tcggacagcc ggaaggtcct tccattcagg gcggctcgtg agggaagcca
    55381 gttccatttt ggtgcgggca acagtaaacc cgcacggcat cagcacgatg acgtccggtt
    55441 cggaggcagc gaagacgtcc cagtcaattt tgtgagaggc ggcaccggcc tctgccagag
    55501 catcgatacc gcctgccgct gccaccatgt cgggtaccca atgaccggcc gtgtataaag
    55561 gggacagcca ttccaggcag gccaccctcg ggcgcagcga ttctttctct atccgcgcgt
    55621 gcaccgcgtc gagcctgcca cgaagttggc tcagaaaccg ggttgcggtt tcagcctggc
    55681 ccaacgcgtt gccaagcgtg gcaatgtcca acaggatatc gtcgagccgt cgcgggttaa
    55741 gagtcaccag gcgcggaggc ggcgccagct tgcgcaagat ccccgttagc tgtgtcggag
    55801 tgatcgcaca gacatcgcac aaattctgcg tgatgagcac ctcgggcttg gctgcaagaa
    55861 gccctcgttc gtccagttcg tagaggcctt caccggctga gagcattgtg ccgacttgcg
    55921 catcaatctg ttcactggct agtgaccggc tttcgatgcg agcgcggacc atgacggggg
    55981 cgtgacttag ctgcggaggg aaatcacatt catggctgat gccgaccagt tgatcggcga
    56041 gacccagcgc cgcaacgact tccgtggctc cgggtaccag cgaacaaatt ctcataaacg
    56101 gccttgagcc ggtccccaga ggcgcgatcg gaggtccgag agtctggtgt ccgccaatga
    56161 atgcgtgatg gcgtctgcct cgccgagatc ctgcactgtg tgggtgttgg cgactgcaag
    56221 gactttcatg ccggccgctc gcgcggccct gatcccgggc agggaatctt caatgaccag
    56281 gcagtcgtct ggagttaatg ccggttgcga cggctgccga ttcaaggcgg ccatggcatg
    56341 gaggaatggc tccggcgcag gttttccccg agtcacgtcc tcagcgctgg tgatatggag
    56401 aaaggctttg cggagcccgg cttcctcgag aatcaattcg atttcgttcc gcagcgctcc
    56461 cgaagcaatg gcgagtggat accgggcggc ggcgtcatga accaactcgc gcacgccagg
    56521 gaaaatcgcc agatgttctc tgaccgcgga cagatacgcc tgggccttac ggtccatcaa
    56581 ttcacccagg agtgtgggcg acgcctgccg gtgatgcgac tgtaatgcgg ccaggaaaca
    56641 cccgcggtcg tcaaaaccga gataatcggt gtagtactcc tgttctgtca gggaaatatc
    56701 gatgccggcc aaaacctgtc gaagggccgc gaaatgcagc ggctcggtgt cggcaattac
    56761 tccgtcgaaa tcaaaaatga gggcgcgtaa ttcgctcatg aggtccgggc ctatccttat
    56821 gcggagatcg tctgagaccg gtttgccgat tatgcgggcg cagcattata gcaagggtgg
    56881 cgtggcaaaa aaagacgacg gcccgttcca agggaaacgg gccgtcgttc gcgaagcgaa
    56941 aagcaggaat gtacttagcg tttcagcctg agactgcgtt cagaaatcca accttttttg
    57001 ccgtcctggg tgcgcactga gtagatccag ccgtttttct ggtattcccc gtcaagaatc
    57061 gtcagcgtgg tgctggccgg cacggtggtg ctggttttcg tgccgatgga atcggcaaat
    57121 aaaggcatcg gagacttggg cctgctggga tcagcgatca ccatcacccg ctgaccttcc
    57181 gcatacaacg gacccgccac tctgacgggc gccgaagccg gtttcatcgc aggaacggga
    57241 gcagggacca cagcagcggg tgctggtgcc ggcattggag tcggggcttc cgcgggcggc
    57301 ggtggcacgc tatcgaccac ggcgggcgga gccatcgatg gcgcaggtgc cggcgcaggt
    57361 atcggtgccg caggtgcctg tacggcgggc ggagccggtt tcggggccgg cgccggcggt
    57421 cgctgggcca cgggttggct ggtctccgtg ccaccatcca gatacggctg gatcatctgc
    57481 atggctgcgt caggatccat ggccacatag ccgatgcctc ctaccagaag gagcagcagt
    57541 atccagagga agggaccctt cttggatcgc ttgggcgcat tcatcggcgg ggccggtggc
    57601 ggcacggctt catccaaatc gtcttcagtg aattccaaat caggctcggg ctggcttgcg
    57661 aacagcagac gccagtctgt ccacacactg cctatcgcag gaatgcccgt agatgacacc
    57721 atcggaggac ctcctcattc ggaatcatgg cgagagtctg tctaatcgta ggtacttatc
    57781 acccgttctc cgacctgtca attttgttcg agaaaacgac gacatgactt tccgggcacg
    57841 gtgcgcctcg cgagcggagc tcacgacgcc tgatgagtca gccgcgcagg cgaatacacg
    57901 atcattccga tggggcattc gccgggtcat cgcgcacatg cacaagggct tgcaagttcg
    57961 aacttccccg ttgcagaatc gcgcaccacc tgtgcacgga gaccgatgag atcctaccga
    58021 gaagagctct ggttcgagac gaaaaccagg cgcgcgtatc tgaacatcac ggcccaggtt
    58081 gaagccgccg tgcgaaagag cggcgtgcgt gaaggactgg tgttggtgaa tgccatgcat
    58141 atcacggcga gcgtctatat caacgacgac gaagccggac tgctccagga ttttgacacg
    58201 tttctggaac ggcttgcgcc gcacgacgcg acctatcgtc acaataacac gggcgaggac
    58261 aatggtgatg cgcacatcaa acggcagttg atggggcggg aggtcgtggt cgcgatcacg
    58321 gatggtaagc tggacttcgg tccctgggaa caaatttttt atggtgagtt tgacgggcga
    58381 cggcgcaagc gcgtactggt aaaagtcatc ggagaatgac gacgcgatcc ttgtacggag
    58441 acgctgcgtc ggccggagcg ataaggaacc gcattcgcgc ttgccacgta tgggaaagtt
    58501 tggcaacatg gacaacagga tgaacgacga ctacctcgca acacgaacgc gcatcgacct
    58561 gggaggccag ccatgttgag ttggtcacga ccggacccgc tgacgcgaga tctgatccat
    58621 ctcattaacg cccgacgcca cgatcacggt gataccgacg cggcgatcga taccctgcaa
    58681 gccttcattg agcaggggcg ggagcaggat ctgttgaccg tgctggccgc actcgacgag
    58741 gagatggcgg aatggatgtt cgacctggtc gcggaggcct cctgtgccgt gatcatggac
    58801 ggacctgatg aagagaaaaa cgcctatgcg ttgatggcgg cactggagtt gccccttcag
    58861 gccaatctgg gccagcccct cggattaaag atcgaccccg tggcgacggc cgatctcctt
    58921 caccggcatc ttcacattcc cgcaggcgtg gtcgtgcgtg tcgagccgcg gttgctgacc
    58981 gatgcgacac tcgaagggct gacgctccaa acactcaacg agcacctcgc aagtatcgcc
    59041 gattcccaac gcacgcaggc cattgcctcc cggctctatc ctccggttga aaatacggcc
    59101 ttcctctttt tccagctgat cgggatgccg gatcccggat tgcccgactc attggaagag
    59161 ccggaacgtc gcgccttgct cgagggcctg gtgacccaat gttccgaact ggcgctggcc
    59221 ccggacacgc tggaggtgcc ggtgtatgcg tcgtatgccc tgttcgatgc ccagaatgcc
    59281 gtgcggctgc atcgcctggc cgacgaaacc gccgctgtct ggcgtgacct cgatccgatg
    59341 gtgcgggctc gcacgatcgt ccacctgcac acgcagtgcg ggaacggggt gtatatgccg
    59401 gtctggacag atctctttga tcccgagttg ccggaggacc atggtccggt gtggacttca
    59461 ccggatgtgt gggtgttcac ggccgacctg ccgatcgagt ggccgggcga aatgctgacg
    59521 aatcgtctgc tcgccgaggg ctgcactcgc ttcgagaacc acatcgtcga agcgcccgaa
    59581 aactgacggt cgctgttccg tttgccgatc gagttggata ctctcataga ggagtttctt
    59641 ggctggctca tcgcgaatcg gcgctgtcgt gctggtgacc ctcctcagtt gtctggtgtt
    59701 aggcggtatc tggggagtcg tcctgtttgt ccttccgcaa agcgagtctc ctcccgaggc
    59761 cctgccggtg tccgttccca cgcctccgct tccggttcct gccgcgacgc agcccccaga
    59821 ccgccctatg atcgatgatg ctgctcgccc cggcgtggcg aatgctgagc ctccagtcgt
    59881 cgtgccgcaa ccttccgttc cgcctgttgc cgaggccgcg cgcatgccca tcggacggga
    59941 ggtcaaatgc gcgctggaga tggatgcgct ctgtcctgaa gaggaagggg accggcgggc
    60001 ctgtttgcaa cggaaggcag cccagatgtc tcagccttgt cgcccgatgc tgcgcgacaa
    60061 gcttgctcga atgaaagaaa attggcagca acttcgagtg gcgtgcgaag cggatcgacg
    60121 gcagttttgt cgtgaggtgc cgtcagcggg cggcgaattg ttgcttcagt gcttggaatc
    60181 gcatgcgcaa gaagtgtccg atcagtgctt tcaattcctt ccgaagcgcg gtcgtctgct
    60241 caattagtca ggcggcgtgg ctccgttcgt ctcgcaacac aaagcgcatc gtaattcccg
    60301 cgacttccac cacgtctcca tgccgcaggg catgttctcg ctgaaccggc cggccgttga
    60361 ccagaatgcg tttcccgctt tccgtctggc tgaccacata ggcctctccg cggcggctga
    60421 tcatcgcggc ggttttcggt gcaaaccagc cggtgagagt gatgacggca tcatcctgcg
    60481 cgccgatcag tgagacctgt ctcgtcaaag gatagtgcga ttgaccggtc cggccagaaa
    60541 gaatttccac gattcccacc ttgcgtgcgg gagccggtga ctcgcgtggc gcctgagatg
    60601 gcaccaccat ggtcttgtcg gccacgagca tgtctgcggg gatgatgggt tctgctgcca
    60661 gtcctggctt ctcgcgaaaa atcacccgat gagttccaaa ccggagcgca tcggcatcct
    60721 gcaattgacg ccgatcgatg gggtgttcgt tcacgaaact tccgttggtg ctgctgaggt
    60781 cctcgagaaa cagcacacct tgaacttgga cgatgcgggc atggtgcgca gaaaccgccg
    60841 gatcttccag gcagagatcg ttatccggct tgcgaccgat ggtgaagggg gtgcgcctaa
    60901 tttcacattc tctcgtgccg ccatgagggg atctcacgag caggaggggt ggtatcgagt
    60961 gctgagacat ggaggctcct tcaaagtcta tctaggctgg gggcgaaacc aattgggtgt
    61021 aaatgcggtc ccagaggctg gtgggccgtt gcgtcaccag cgccacgatg acggtcgcgt
    61081 tgtcggtgcc gccggcggca ttcgagagag cgatgagacg gtctgccata gcctgaggat
    61141 cgatggattc ctgactcact cgcaggatga catcggccgt cactccagcg gtcaatccat
    61201 cggagcagag taacaggata tcgtcgggca gtaccggcat ctcccctaac tcgacgtcca
    61261 ccaggggatg cacacccacc gcacgggtca gcacatgtcg atgcggggcg cgcgccatgt
    61321 ctgcagcggc caagagtccg gattgcattt gctcttgaac gagcgaatgg tcgaccgtca
    61381 gcaactgcac tgcgccgtca cgaatcagat acagtcggct atcgccgacg tgagcgatcg
    61441 acagaacggc tccagtaagc cggactgcga cgaccgtcgt ccccatcccg gcatattcgg
    61501 tgcgtcgtgc cgcttcctga tgcacccggt gattggcgag gcgcaccgcg ctcgccaatc
    61561 gattggtggc cgaagaaaac tctggacggg aaatgcctat caacgggagg gaccggtctg
    61621 tctccgcctc cgcaagatgg tgagcaatag tctcaaccgt gcggctgctg gcgatttccc
    61681 cggcccgatg cccgcccatc ccgtcgcata cgacaaaaat tccggcggcc ggatcggcat
    61741 gatagcggtc ttcgttgagg cggcggacca ggccgacgtc cgaagcggct ccatgtgtga
    61801 cgtgccaggc gtgcatgggc gtggttatct cggcatcacc tggaagacgt cacggaggtc
    61861 ttgcgccatg tcccccgcat gccggtagcg gttctgaatc tctttctgca gcgcccggtc
    61921 caggatgctt tggacgcgcg tcggcaggtc gcggcggtac ttcatcaacg gggcatgtgg
    61981 agacttggcg atacgcatga ccagcgccgg catattctcg gcatcaaagg gccgttctcc
    62041 cgtcaaaagt tcgaagagca caataccgag tgaaaagaca tctgagcgtc cgtccacgtc
    62101 ttttcctgtc gcctgttcag gcgacatgta ccgcggcgtc ccgacgatca tgctgctctg
    62161 tgtttttgaa gcgctcgcca tcttggcgat gccgaagtcc atcaccttca ccaggcgttg
    62221 cttgaggatc atgatgttcg gcggtttcac gtcacggtga atgacgccct gactatgcgc
    62281 atagtccagc gcttcggcaa cggtggccac gatctgcagc gcctgtttgg ctgggaccag
    62341 cgtcgatttc tggcagtaac agttgaggag gactccctcg agatactcca tggcgatata
    62401 ggccaggccg tcctgctcgc cggcgtcgta gaccgtgatg atattcggat gggacaggcg
    62461 cccggtggat tcggcttcgc gaaagaaccg gtctcgaaac tctttcaggt cttcttcctt
    62521 gtcgatgtcg tccagccgca tggttttaat ggccacaaat cgctggatcg tcggatcttt
    62581 tcccaggtag acgactccca tggcaccgcg ccccagttcc ttgagcacct ggtatcgtcc
    62641 cagcgtagac ggcacgccgt ttccgttgcg cacggctatg ccggctgtcg ggcggggggg
    62701 ggcggcggga atggcttcgt ctgccgcacg cacctcgtcg ggcttctccg ctcgcaccgg
    62761 ttcttcggca accgcccggc ggcgtggacg ccaggcaaac atgctggtca gggcatcgac
    62821 atgacggacc caggcaaacg tcagccaggt cgctgagccg atgaggccgc cggtgaccaa
    62881 ccaggtatag agcggctgtc taggcgggcc gagcacagat atgccgtgtg cttcataggc
    62941 ctggaatgcg gcaatgacga tcagaaggga ggcggacggg aaaagaatgg cgcggagaaa
    63001 ggtttgaccc ttgccgttgt ccttcaggtc tcgtctggct cgcgaggccg cagaccagat
    63061 catgagcagg cagatgccgt ctgcaatgaa ccgaacgact tgcgcgacgt tcatgcccaa
    63121 cggccggagg taggtgtgcg taaacagcgg gagggtcgcg agaaatggcc cgaccagaac
    63181 cgcaaggaga atgacgatga aatacgggcc gatccagccc caagatgtgg tcatgtgttg
    63241 gcgcggtacg tcgttgtagc cagtgtaacg gaaggtcgta ctgtgtcaac gaaacgaggg
    63301 aatcacgcac cgccggggca gagtctcgtg gcatccggat gaggacagag gaggcgtgtt
    63361 tgggagaaaa gggctcagga cgaggtggcg gcttttctga gtggatcgag caatttctgt
    63421 gagactttga gctggccgcg gccgatcccg acctcaatat ccggtttggt gacgccgtgg
    63481 aaatcgctgc cgccggtgac gaggagatcg cattgcctgg ccagatccag gtattcggtc
    63541 gtttgactgg gcgtatgggt gctgtaatga acctccattc ctcccaatcc ggcagccttg
    63601 aggtctctca caagtacccg caacccttcg gcggatgttc tgacccacgt cgggtgggcg
    63661 agcaccggca cgccgccggc ttcgcgaatc catcgcacgg cttcggccgg ttccggtaat
    63721 tcccggtcga caaaggccgg gcggccgttt gccaggtatc gatcgaaggc ctctttcgcc
    63781 gaggtcacaa attttttctc catcaaaaga cgggcgatgt gggggcgccc cacggattcc
    63841 gtgcctgcca gggcccgcac ttcttcataa gtaatcggaa tgcccaattc gttgaggcgt
    63901 tgaacaatct taggattacg aagatgtcgg ctgtcgcgca atgtgctcag gcgttgcgct
    63961 aataacggat cggtccaatt caaaaaatat ccgaggatat gcagttcgct ctctccgagc
    64021 cgcgaactga tttcgacgcc gggaactacc tcgatgccga gttcttttcc gatagctgtc
    64081 gcttcgggaa tgccgtcgac aatgtcgtga tccgtgatcg ccagcgccgt gaggccggcc
    64141 tgcttggcaa aggccatgac ctcggccgtg gagcaactgc cgtcggagtg cgtggtgtgc
    64201 agatggagat caatgcggct catggtgatg tctctggccc cgttttctgc gcgaccagtc
    64261 gcgcggtgta gcatggcccg ctgccatgac ctccgtcgtg ttcgccctcg tcatagtgaa
    64321 gaacgcgcag gggcgaggtg aggcggagga gttcgttctg cgccaggcaa aactcccatc
    64381 gccgcggatg ctgatggcga aaataattgt cgatggtaaa tgtttcgtag aggaggatgc
    64441 cattgggttt cagggcgtca atgatgacag ggaacagagg ccgatgcagg taaaaaaaga
    64501 cgacgatggc atcataacat tcgtggccca agctgggcgg gtgatccgca gaggcttcca
    64561 ggttgaggac gcgtgtcgtg agaccggaga gacgctgtgt gcccgcagcg gcttccaatg
    64621 cggagagggc gtcggtgtct cgatcgatgg cctccacctg tgacccctgg gccaggaggt
    64681 acagggcatt ccgtccgcgt ccagcggcga cgtcgaggat gtgtcccttg gggagacgcg
    64741 caagttgttg gatgagaaac ggtgcggcgc tcagtgcctg gtgtgttgac tgatgggcgg
    64801 cacgcgtgcg ttcaaaccgg atgccgactg acccggcgac catggccagc ggggcatgga
    64861 cttttcgaat ccagatgcgg acccgatcag ccggaaattc cgcgaataac atgccgacca
    64921 attgctcggc cagggtttca agaagctggc aggtgaggct cgtgctgagt gcgacgattc
    64981 gttccgctac ttgagcataa tcgatcgttt cactcaagcg atccgaaacc gcggcggctt
    65041 ccacggcggc gtccagttcg aggtcgacga cgatcagctg gggtttgcgg cgttcttcct
    65101 ccgtcacgcc gcagcggccg tagaactcca ggcgttcgat gacaatccgc tctgccatgg
    65161 cggcattgtc gtagatgggc tttcagactg tcaagcaaag cgggcgcgcc attgagctag
    65221 gagcgtgcga agagacgctt cgtgagccaa ttggcgggcg aggcgaaccg gcgggcagag
    65281 gcgacgatcg tcgcaggaga acacttcgcc gtgaagtctt gcaccatccg tctcgcaccc
    65341 ctgtacagaa actcggtcac gtcactgact gtcggttgtg gggtacggtt cagatgaagc
    65401 tgcttcaatt cggccttgta ggctttgcgg cggcggcgtg tggcatgaat cgccacggcc
    65461 gcgacaacgg tcaacagcaa gggattcgag gggatggaga acaaccaccg ccaccattca
    65521 tagaatgcca gcacggtcag gcagaggggc agcgtcaaca catcaaccca tgccgattgt
    65581 acggcgcgga tctgttcgtc tatcgattga ccgttgaacc ggcggggtat gctttgcagg
    65641 gacgtgcgtc ggtttttcga ggtgaagact cgcgcgggga gaaacattcc gggtacctcc
    65701 agtagtgaac gacatccttc tggaggcatt gtaggacagg ttgcacaatc agaatagaaa
    65761 agacggaatt tctgcgggtg tcgactcggt agttttaccc gtatgnnnnn nnnnnnnnnn
    65821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    65881 nnnnnnnnnn nnnnnnnnnn nnnnnacgaa tcgacgtgcc cgccgtggct ttcaattcag
    65941 tggagccgat gcagcacgcc ttcggcgaca acggagctcg gatcgccacc gacaatcaaa
    66001 tccttatcac cgaaatggga ggcggtccgc gtcgtcacgc gcatcttcat tccctcgggg
    66061 accagcgtgt actcaaacaa ggcggcttcc tggcgttggt ccatataccc ctgactggcg
    66121 cgttcgaacg agccgataat actgatcgac acacgccccg ggccctgttc gaaacgcttc
    66181 acagccatcc gttgcacacc gccacctggt ggcacgtatt caatcgtttg cccgacgtgt
    66241 gacaattcca cctggcgtgt gacgcccttg ttgttggtat aggagtagag agggaggccc
    66301 tgcggactga agtcgatttg gacttcagcc cggctgccgt ctgggctgct caggacccct
    66361 ttccacgcgc cggccaattc cgcggcctca gtggagccgg atacggccag gacgccgatg
    66421 atcgctgcga tgtgcacgaa gtgtctcata gtcatgagcc taccctcctg tgcgatggcc
    66481 ggtcgcgcgc cgagctctct tatactttga atggctgttg ccggagaaag cgccccgccg
    66541 ccccgagcgt aaacagattg ggcgactggc tggcgggcag ccccgccttc actgccgcat
    66601 ggaatttggc atagttcccc cgatagttcc cgtggttcca cgccttcagc acctgttcgg
    66661 taaaggcgcc attgtggtcg ccgtccatgg acgtctggtt gtcctggcag cctgaaatga
    66721 gaatcacggc aggatgaaat tctttggcga tggccgtgag gcgggggctc acggccagtt
    66781 gggccagcac gctgtccgga tccgtcgtcc cggccttgcc ggcggctttc gccacgtcat
    66841 gctgcaattt gttgtagaac gcctgatgtt cccgatacgt ccgaatggcg acggcagggg
    66901 gcatcatttt cgagcgtccc gtcaccttcg tccgatcaat cggcggtggg acggcgcggg
    66961 tgaccgtacc gctatggcag ctgtccgaca acacaaggat tcgcacgccc gccgcaaagc
    67021 ggctgagttc gaagtacaat tcatcgtcga tgagttgacc gtcgtacaga caccaggtct
    67081 catcctgctt gtcgatttct tctcctgtca cgtcgggcac ctgaccgcca tggccggaat
    67141 aggtcagaaa aaacagatcg ccttgtttga gctgcttcgc ggcttttcgc atcgcagcca
    67201 gcattttggc gcgggtgcct tgtttggtca gcagcacggt ggatttcatg ccctgcgatt
    67261 tggcgatggc ggccatgtct ttggcatcga actcacaggc ggccagttca ccgctccagc
    67321 cgccataggc ggcgggactc acggcgttga ggccgatgtg taacgagagg gctttcggct
    67381 ttgcagcgga tgctgtggtg gttttggcca tacgtacctc cttggtcggc tggctccatg
    67441 tcttgatagg ttcggatgaa gagcaaatga tggcggtacg gtaatcgtta cgccgaaatc
    67501 tgtcaacgga gcttcgtggg aaatactggt attgtggccg tccggtgacg ctcatggatc
    67561 gggacgagat gtggcgatga accacatgat taggtgtcct cctcctcgat ttcttcgatt
    67621 gactccttga tcgcagccac aaatgtttcc ggtgcggcga gggcctgcgc agtcagttgg
    67681 aattcagaga ccagcacccc atctttatcc acgacgatca gccgataccc ttgtttcacg
    67741 accacgacct ttctctgcgc atttcgcagg cagatgctat tgacgagagg ccctacgggc
    67801 aggctactgt atcgaacgat cagccgtcat gtcttcgttg ggaggcagga tgaccggttg
    67861 gtgttcgcca ttcaggcctg gtaggatgac acaatcacca gtacgattcg atcgtccaag
    67921 gaaaggatag tcacttgcat acaaccaaac tcatagccgc atttctcgtg cttgtgttgg
    67981 tcggcgtgcc gacgatttct tttgcgaagg cgcgaggggg atcaggggga ggattctcca
    68041 gcgggtcacg aagcggatct tcaggaagca tgggcagtcg tggatcgcgc acctatcagg
    68101 acaatggggc caagcccatc gaacaatcca cgacagccag gccttccgcc accccgccgc
    68161 cttcgatggc gggcaatcct tcgactgcat ctaggcccgc tcccgctgcg caaccgtcat
    68221 ggtttcaacg caaccccctt ctggccggca ttgtcggagg tcttgccgga acgtggctcg
    68281 ggcacatgat ttttggtgcg accgacacga gtgcccgcac gacggaaggt ggtgagggcg
    68341 aacaggccgc cgcaggggca ggctcaagcg gcatgttgct gctgttgatg gtgatgggtg
    68401 gcggtgcact ctggtggtat atgcggtcgc gacgtacacc ggcgccgaat ttttctgggc
    68461 tttcgcgaac cagtgtcgcg agcggctcat tgctggccga atcatcgcgt gcaggattcg
    68521 gggcggccgc tgttgaagag gagatgacgt caacagacaa ggcggcgttt caacaagcgc
    68581 tgatcgatat tcaaaccgcc tggagtcacc aggacctcgc cggcttgcgt cgcctcgtga
    68641 cgccggaaat gctggattat ttcagcaccg gtctggccga gcaggccagt gagggtgtcg
    68701 cgaatcgcgt ggaagatgtg gtcttaggac aggcggaagt ccgtgaatcg tggagtgaag
    68761 gtgcgacgca atatgcgacg gtcagtctct gctggagagc caaggactac accatctctc
    68821 tggcgaagca gccgggcgaa cagggctaca ttgtggaggg caacgctgag ctgccgatcg
    68881 aaacaacgga ggtatggacc ttcatgcggt ttggagccgg gaagtggctt ttgtcagcca
    68941 ttcagcaagt gtcatagcgg aatgttgaaa aatcccgcca gcttcgttct cacgtcgttc
    69001 agaggatcaa cgtacggctc gagtacgtct cacctcttcc ctcgttgcgg ccttgctgga
    69061 cgggattttt gaacatcccg cgggcctcgt gaattgcgaa tggatcggta ttgttccttg
    69121 gggtagtcac accggtggct gcccccgttg ttctttggtt agcgtgtatg tgatgcctgc
    69181 cgcaaacggg tagcggcggt gtcttcaata ggcaggcaac cttgggcttg agaaattgcc
    69241 tcgagcagcg catgccgctc ctggtccgtc tcctgcaccg gcggcatgct gtgcacgaat
    69301 tgttctgccg cctccaccaa gcgttgcagc ctggtggcca tttcgtccga cggtgcctgg
    69361 agttcggacg aagtcttgcg agccagtgac cgctttcttc tcggcccgac atagatctgc
    69421 ggggccacgg gattatcctt ctccaattcc ggtcgtctcg atatcggcat gcgtcctccc
    69481 aaatggcaaa cgagtaatgc ccactctact cgattctgcc gcgcgtttgc atggtcgaaa
    69541 tcattcttgt ctcaatggag aggtgagcct gaatccgtca aacgattcat ggcttgtcac
    69601 actgattatc tggaatcgta accgtgtcat ggctgcgatt gacggggcgc cacagggatg
    69661 acgggtgagg tggtatgtcg actcagatag accgttaggg gaaaacgagt gcaccggtca
    69721 actgtatgga aggattcagg gaaatcgcat cgcctctgat cgatgcatca gtcagtctct
    69781 catttctcct tcggtgaatt ataggcacga ctgttgcgtc agtaattgcc gttgttgtaa
    69841 cgcgaggtga cattcattcg gccaaggctg tcagcgatgc cggccagagg agtgaagcat
    69901 gcgccagtct ctcggaatgc gttgctattt taccgccttc gccatgtttc tttcggccct
    69961 cgtcggccgg tcctgggcgt ccaggagata ggttgcgcta gcaatctccc ggtttcgtgt
    70021 ctgtcagcgg tgttttccct cacgcagtcc tttgtctgac tgaattcggt tccttcctcc
    70081 ttgatcccgc tcggaccaga tactcgcctg ccgttcaacg cgctcgattt cgttgacgag
    70141 gcgc
//
LOCUS       DAOQDS010000013        64471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_135_length_64471_cov_26.038282, whole genome shotgun sequence.
ACCESSION   DAOQDS010000013 DAOQDS010000000
VERSION     DAOQDS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 64471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64471
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135_length_64471_cov_26.038282"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PK589_04755"
     CDS             complement(<1..683)
                     /locus_tag="PK589_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNJ18938.1"
                     /translation="MPTPKIARPTLILGVGLLLLVAGASVWWFYFRIPPLVGFGSGNG
                     RLEVQEIDVATKFPGRIAEVLVDEGDSVQVGQVVARMDTSSLNAQVREAEAQVQRAKQ
                     GKITAKAVIAQRRSETLLAERDLERARALYVNANISVKDYDRARSTMDTAKSAITQAE
                     AQLAEAEAAIKASLAQSERIQADLKESVLTAPRSGRVQFRLAEPGEVLAAGGKVLTLI
                     DPTDVYMTV"
     gene            complement(702..3122)
                     /locus_tag="PK589_04760"
     CDS             complement(702..3122)
                     /locus_tag="PK589_04760"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNJ18939.1"
                     /translation="MHTKTFFCIISVIFLLSWRSNVEAKSLPVVQVGIVRDGPSELVP
                     ELRDLIQKEILEITRGEFEVRFPNDLQFDGGWSVQGVKQAIDRVLAQPRVNLVIALGI
                     LASQEVSQRRQLPKPVIAPLILDPHLQGLPFKKGTSGMKNLSYLVTGKGFERDLEAFK
                     EVFPFTRLTLLADRVLLEAFPPVKDRARKMAETYGVKLTVVPVETSAEAAIGSIPPDT
                     EAVYLSPLPRLLAGEFDRLVLALNRRKLPSFSAVGQRDVQRGITLALSPELDLGRFAR
                     TIAANVQRILSGTDAGRLEVAFIRGEQLVINMATVRAIDKWPSFQVLSEAELVNEDAA
                     GTSRRLSLFEAVRESAEANLDLAAASRKVAAGVGQVGQARSGLLPQVLVGTSGVLNGR
                     GPDTFGTGGTPGQAAIALGGFRQLLYSDDAWTKYTVQQKTQLSREEEWNQLRLDIAQD
                     AALAYLTVLRTKTVRRIQKDNLKLTRANLDMAAARESVGYALRDELFRWEAEVANGQK
                     AVVSAEAQERQAEVDLNRILNRPLEERFQTVEQTLEDPGLLGDTRQLFAYIENPRGFQ
                     VFRDFEVEEGIQAAPELRRLDALSAAQERTITNAQRAYWLPEVAIQGAGFQQYAQGGG
                     GAGSLTVPLGPVGFAQTQNNFYVASIGLTFPLFQGGYKDATALKAREELRQVQFERAA
                     VALQVEERVRTTLYQTGASFPSIRLSRKAAESARKTLDLVVDQYSRGAVDIIKLLNSQ
                     NAALTANQAAANAVYEFLIDLAKVQRAVGQMSFFRSSEERAAWFERLKTHFVNAGVSP
                     VLRDDPRN"
     gene            complement(3291..3566)
                     /locus_tag="PK589_04765"
     CDS             complement(3291..3566)
                     /locus_tag="PK589_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18940.1"
                     /translation="MPRPSRPGATLEKQQPHLHDEARHIWESYKKGVIRDISFRQDRL
                     GVATTLERNCAGSLKPMPCTFRNDVEKAFELMRTKADGMLKPLITSA"
     gene            complement(3627..4643)
                     /locus_tag="PK589_04770"
     CDS             complement(3627..4643)
                     /locus_tag="PK589_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNJ18941.1"
                     /translation="MRGAVLYGPRDVRFEERDAPKIIEPTDAIIRIALTCVCGSDLWP
                     YRGLQPITEPTPMGHEYCGIVEEIGGAVRSVKPGQFVIGSFATSDNTCPHCQAGYQSS
                     CVHREWMLRAQAPVLRVPLADGTLVPTPGVPSNDLLPSLLTASDVLGTGWFAADAANV
                     TPGSTVVVVGDGAVGLLGVLSAKQMGAERIIAMSRHEKRQQLAREFGATDIVTERGDE
                     GTTQIKELTNGVGADSVLECVGTQESMMQAILATRPGGSVGYVGVPHGVLLDGEKLFY
                     SHVHLHGGPAPVRRYLPKLIDLVLDRKINPGKVFDLTLPLDQVAEGYRAMDERRAIKA
                     LLRP"
     gene            complement(4782..5192)
                     /locus_tag="PK589_04775"
     CDS             complement(4782..5192)
                     /locus_tag="PK589_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNJ18942.1"
                     /translation="MNKPEFFVTPGYGERNLKLFHYSQAVKIGNRLELSGQGGWNDDW
                     EFPESITDEIAQAFRNVERTLAVAGARWEYVIHVNSYHVGLDGDQEEINQAMTERFRH
                     YMPGHAPVWTCLGVAALGDPKMRVEIRVTAILPE"
     gene            complement(5416..6504)
                     /locus_tag="PK589_04780"
     CDS             complement(5416..6504)
                     /locus_tag="PK589_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ18943.1"
                     /translation="MCITPALGKPAAGPAPICDQNRTSSPLITQRRTLGSGKHALQVS
                     ALGFGCMGMNYHRGLHPDRDAMIKLARQATERGVTLFDTAEVYGPFVNEELVGEALAP
                     FRGQVVITTKFGWKIDPGSRTSIGLDSRPQHIKEVAEASLKRLKVDAIDLFYQHRVDP
                     DVPIEDVAGAVKELIQEGKVKHFGLSEAGAKTIRRAHAVQPVTAVQSEYHLMWRAPEK
                     DVLPVCGELGIGFVPYSPVNRGFLGGNLNEYTRFDSGSDNRKTLPRFTPEAMRANLAI
                     VEALNRFGRTRGVTSAQVALAWLLAKQPWIVPIPGTTKLAHLEENLRTADLKLSAAEM
                     SEIEGSVSKIEIVGDRYPAEQQKLVSPD"
     gene            complement(6674..6937)
                     /locus_tag="PK589_04785"
     CDS             complement(6674..6937)
                     /locus_tag="PK589_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNJ18944.1"
                     /translation="MGSAFGVDEIMLCGGGGLNWSFIRADLCDEVSIVLTPAADGTKG
                     AQTLFEADERYTQPLPTAFKLKEVQPLDDGSVWLRYTVEGPIN"
     gene            complement(6961..7407)
                     /locus_tag="PK589_04790"
     CDS             complement(6961..7407)
                     /locus_tag="PK589_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18945.1"
                     /translation="MDRPRIFIHMHMSLDGKIVGSYLPTDVGMASQRQYYNLFLGPDR
                     HFRNHKGWLSGRTTSEDNFTHYRKLKLDEFAPPMPEGDFIAVDDAPMHYFSVDPSGVL
                     AWETNSITYFDTTAHVVQILSGKATNAYKDFLRRKGVSYTSPARTL"
     gene            complement(7432..8550)
                     /locus_tag="PK589_04795"
     CDS             complement(7432..8550)
                     /locus_tag="PK589_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ18946.1"
                     /translation="MKYRKLGNTGTVVSTLALGTMYFGDETAENDAFAILDAFLDAGG
                     NLVDTSDVYVGTVAEQIVGRWLAARPREITDRVIVATKGRFGDDSDPNAVGLSRRHLH
                     RALDASLRRLDVETIDLYQLHASDMHTPVEETLSFLDDAVRAGKIHYVGLSNFTGWQL
                     QLMVSTAKATGVQTLVSLQQQYSLLSRECEWEMNPAARHNGLSLLPWSPLAGGFLTGK
                     YQRGGTPASDSRAGSGKDLYQWISADYAGSDQNWATIETVIRIAKEIGATPAQVALSW
                     IANRPGVAAPIFGARNLRQLDENLAAAKLTLDENATKALEVVSAPRSNGYPYGAFGAG
                     QRNRDLRDGNPAPGLPVAGGSDHPLGRPSLTVTSRVSD"
     gene            complement(8664..9260)
                     /locus_tag="PK589_04800"
     CDS             complement(8664..9260)
                     /locus_tag="PK589_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ18947.1"
                     /translation="MSASTPMTSSATSAGQLLARQRRHASPELLAAALLLLGTVAALQ
                     SEYSLWWREPEQELLPTLEELSIGFVPFSPLGKGYLTGKIDETTTFDSTDFRNTVPRF
                     TAENRKANQAMVELLGRIAERKKATPAQVAIAWLLSQKPWIVPIPGTTKLHRLEENVG
                     GAAVELTPDDLRDIADAAANIAVHGERYSEAAQKMINR"
     gene            complement(9274..10176)
                     /locus_tag="PK589_04805"
     CDS             complement(9274..10176)
                     /locus_tag="PK589_04805"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ18948.1"
                     /translation="MQRVSFKNRNIIMAGSLCQPRDFKDGARYPAVVVVHPAGGVKEQ
                     TAGEYAKRLAAEGFVTLAFDTSYQGESGGEPRYLDEPMNRVGDIYSAVDYLTTLPFVD
                     AERIGILGICAGGGYSVKAASLDRRIKAVATASAANVGNAARKGWDGKSPMSALPGTL
                     EMLVNQRTAEAAGAAIAYAPYVPNVGESSPFRDLREGSDYYLTPRGRHPNAPNKMLMT
                     SLMGWIAFDAFDLVDPLLTQPLMICAGSEADSLWQSQELYAKAPGSSKELFIIERASH
                     MGLYDGSGREAVLGKITPFFRANL"
     gene            complement(10215..10472)
                     /locus_tag="PK589_04810"
     CDS             complement(10215..10472)
                     /locus_tag="PK589_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HNJ18949.1"
                     /translation="MNRVLVITYPKKDDRIASVMAAEYAMTNSWRDQGIIEHLFAKEN
                     DGGGVVVFNETSVEKVQALIAQLPLFPFFEKVEYFPLNKIY"
     gene            complement(10508..11572)
                     /locus_tag="PK589_04815"
     CDS             complement(10508..11572)
                     /locus_tag="PK589_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ18950.1"
                     /translation="MKTPLLKLNNGVEMPALGLGVFQSSPEDTIKAVATAIADGYRLI
                     DTARAYKNESQVGEGIRNSGINRAELFVTTKLWLTDYGYDAALRGFDGSLTKLRLDYL
                     DLYLLHWPTKNFDATVQSYKAAEKLLAAGRVRAIGVCNHTEQQLEALIARTDVVPAVN
                     QVELHPYFAQKALRQTSAALGIVMQAWSPIGGVKRYWPGASATGTGDPLADPVITGIG
                     RAHGKTAAQVILRWQFQNGVVAIPKSVKAARIAKNIDIFDFVLSDSEMTKIDALDTGA
                     RGGPDPTQIDMDSFSDTVDRMHPVRVSRRDAKQTEPLNEHRSIRHSCSYHGCRLRNGV
                     GISRGILGSRRVRRAVRKPQ"
     gene            complement(11869..12054)
                     /locus_tag="PK589_04820"
     CDS             complement(11869..12054)
                     /locus_tag="PK589_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18951.1"
                     /translation="MPVYVNPYWDLDDSHAVSSFMVAPGRVVTRQEVAALLGKAIRHD
                     KMFGLLGAFDVRTNRLS"
     gene            complement(12184..12624)
                     /locus_tag="PK589_04825"
     CDS             complement(12184..12624)
                     /locus_tag="PK589_04825"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNJ18952.1"
                     /translation="MTQTIGIIGSGMIGGQVARLAIAAGFNVVICNSRGPETLAELVS
                     KQGPSARAATFAEVTEAADLIVLAVPFAGYAKLSAEALAGKTVIDTMNYYPERDGVMP
                     EVKTDTIATSELVQKHLSRSRVVRALNNMDFVRLLSRARPKGSA"
     gene            complement(12632..13627)
                     /locus_tag="PK589_04830"
     CDS             complement(12632..13627)
                     /locus_tag="PK589_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ18953.1"
                     /translation="MQTRKLGNSGLEVSALGLGCMGLTSFYNTALPEGEAVAFLRAAY
                     EKGITLFDTAEAYGPFVNETIVGKAVAPFRDKVVIATKFGFDIDLETGRHLGGVNSKP
                     AHIRAVVDASLKRLATDRIDLLYQHRVDPEVPMEDVAGAVRDLIAAGKVKHFGLSEAG
                     PESIRRAHAVQPVTALESEYSLWHRGPEEKILPLCQELGIGFMPFSPLGAGFLTGKIQ
                     ADTRFGESDYRNTEPRFAQENREANMALVALVKDAAARKGATPAQVALAWLLAKKPWI
                     VPIPGTTKLHRLEENIGAVDVVLSADDIAEMETTLAGLTLRGERLSAGELAMTGL"
     gene            complement(14023..15405)
                     /locus_tag="PK589_04835"
     CDS             complement(14023..15405)
                     /locus_tag="PK589_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNJ18954.1"
                     /translation="MSYQSVNPFDGTTVKTFEQLTDAQLETALETAANCFENWRRTTF
                     AERGAIAAKAAAIMRARIDEFARPVTLEMGKLIAESRAEVQLSADIIDYYAKTAERFL
                     APEPLQPGSGEAKIESAPFGVLFGVQPWNFPYYQLARFAAPNLMAGNVVMVKHSGTVP
                     QCAIAFEKLWLEADAPAGAYTNLFISYEQVNRVIEDRRVKGVALTGSVDAGKSVAERA
                     GKHLKKTTMELGGSDAFIVLEDADLDKTVKWAVWAKMNNTGQCCIAAKRYIVVEKLAD
                     RFLEKFKSALSALTPGNPMDKATTLGPLSTEGALVNLLKQVDQAVAHGADLVLGGKRM
                     DRPGAFMEPTILTNIKPDNPAYREEFFGPVALMFRVKNEDEAVVLANDSDFGLGGSIF
                     TNDVARGKRVASRINTGMVFINHPTWTAPDLPFGGIMDSGYGRELSSMGIQEFVNKKL
                     VRVESIDAPA"
     gene            complement(15525..16445)
                     /locus_tag="PK589_04840"
     CDS             complement(15525..16445)
                     /locus_tag="PK589_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ18955.1"
                     /translation="MGLSFGYGPATDKGDAVKLIRAAFERGVTFFDSAEAYGAVNEEM
                     VGEAVAPFRDKIIIATKFGFKDGNSQAGLDSTPARIRLVAEQSLKRLKTDRIDLFYQH
                     RVDPNVPMEDVAGVVKDLITEGKVKHFGMSEAGVAMIRRAHAVQPLTALQSEYSMFTR
                     EPETEIIPTLEELGIGFVPFSPLGKGFLTGKMDVDTKLDANDFRNTLPRFEAENRRAN
                     LAFVELLTTIANRKQVTPAQIALAWLLAQKSWIVPIPGTTKLHRLEENLGAASVDLTT
                     DDLREIEQGVSKLTVQGARYPEKVEQMTNR"
     gene            complement(16436..16720)
                     /locus_tag="PK589_04845"
     CDS             complement(16436..16720)
                     /locus_tag="PK589_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ18956.1"
                     /translation="MKYIKLGNTGIDVSKICLGMMSFGKPGSEHGLFPWAVDFEDAKP
                     IFKKAIELGINYFDTANVYQLGTSEEVTGKLIKEFKLDRDDLVVASAAWA"
     gene            complement(16801..17514)
                     /locus_tag="PK589_04850"
     CDS             complement(16801..17514)
                     /locus_tag="PK589_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNJ18957.1"
                     /translation="MKPQVICHMLSSVDGRLHVGRYTPLTSGKTSTEASAPYYEYSAK
                     FDADAQIIGGRTARESALPLRYEGDVATRPAHFQTFVGHRQSKRSLIVLDPQGTIRYD
                     SDNVSGENLIAVLGESVSQQYLDHLQAQGVSYLFAGPKGDDVPRLLGTLSADFGMTRV
                     LVEGGGTINGAFLKAGVIDELSLMLYPGIDGLAGIPSIFECQGAPGEKPAEGQSMELV
                     AVETLPDGIVWLRYRFHRQ"
     gene            complement(17553..18353)
                     /locus_tag="PK589_04855"
     CDS             complement(17553..18353)
                     /locus_tag="PK589_04855"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ18958.1"
                     /translation="MSKTIFITGASSGLGKATAKLFAVKGWNVLATMRQPAKETELTQ
                     FANVTVLPLDVTRSEQVSETIQSALKSSDVDVVFNNAGYGLAGPMDGIDDARIVRQFE
                     TNVFGAMRVTREFIPYFKQKRSGRFITTTSLAGLIGLPLNSVYNASKWALEGWSEGLS
                     YELAPFGIKVKTVAPGAVMTDFMSRSLDHVPAPGYDAMNEATVKFFVPDMAKISSAES
                     IAQVVWEAATDDQDHLRYVAGDDAIDIYALRQKLGNESFRQHLASLVL"
     gene            complement(18550..19434)
                     /locus_tag="PK589_04860"
     CDS             complement(18550..19434)
                     /locus_tag="PK589_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ18959.1"
                     /translation="MQTTTLSNGLHMPMLGLGVFQAPPDVTAAAVQEALRVGYRLIDT
                     AAAYANEREVGEGIRRSGLARDDIFIQTKVWISDYGYDATVHAFDKSAGKLGVDQLDL
                     LLLHQPLTSRFDLTLGAYKALEKLLADGKVRAIGVSNFMPDVLQRLLAETSVVPAVNQ
                     IEVHPYFQQKELQRLHAEHGILTQAWSPLGGITSYGGGEKRTFDDPTLQQIAREHGKS
                     AAQVMLRWHLQEGRSVIPKSTKPARIAENLDVFDFELTKEQVASIDALDTGVRSGPEP
                     DSITLENYGREIPEACGK"
     gene            complement(19512..20030)
                     /locus_tag="PK589_04865"
     CDS             complement(19512..20030)
                     /locus_tag="PK589_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNJ18960.1"
                     /translation="MGIVGPGGLGHMGVKFARVFGAHVVVFTTSPSKAEDALRLGAHE
                     VVVSRDANEMKKHTGSFDFILDTVPVDHDINQYLHLLRLDGNLTLVGVPSQPLALSSF
                     ALIFGRRSVSGSNIGGIPETQEMLDFCGEHNITADVEVIPIQNVNEAYDRLLKSDVKY
                     RFSIDMASLKTE"
     gene            complement(20003..20578)
                     /locus_tag="PK589_04870"
     CDS             complement(20003..20578)
                     /locus_tag="PK589_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNJ18961.1"
                     /translation="MYKTKAYSAASATSSLVSTTIARRDPTEHDVQIEILYCGICHSD
                     LHSVRNDWSSVMSTTYPIVPGHEIVGRVTKVGSAVTKYKPDDLAAVGCLVDSDGTCPN
                     CTAGLENFCPNQTFTFGSPDKHKTAPVTYGGYSDSIVVDERFVLRAPTNLNGAGTAPL
                     LCSALGSPPTHPCATTASPRVRKWASSAPVA"
     gene            complement(20830..21894)
                     /locus_tag="PK589_04875"
     CDS             complement(20830..21894)
                     /locus_tag="PK589_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNJ18962.1"
                     /translation="MAQTMKAFVMKKLGSVGMMEKPIPDLGPNDAIVKTTAALICTSD
                     VHTVGGAIGERTNLTLGHEAVGVIYKLGSAVKGFREGDRVAVNAITPCYLCENCLRGY
                     TSQCTDMLGGWKFANVKDGSLAEYFHVNSAQANLAPISKGLTDEQAAYCADMMSTGFM
                     GAEHANIPIGGSVAVFAQGPVGLMATVGSRLLGAGLVIAVEAVPERKKLAKEFGADVV
                     IDFTEQDPVEAILGLTGGQGVDSSIEALGAQATFEACIKATRPGGTISNVGYHGEGQY
                     VQMPRKEWGVGMSDKVIRTGLCPGGAERMKRLMRLLETGRVNPLPLTTHRFRFSDVEK
                     AFELMRTKADGMLKPLITFA"
     gene            complement(22029..23087)
                     /locus_tag="PK589_04880"
     CDS             complement(22029..23087)
                     /locus_tag="PK589_04880"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF003251.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HNJ18963.1"
                     /translation="MRKVKVAVNGYGVIGKRVADAVKQQEDMELVGVADVVTDYRIKT
                     AAVLGLDIYAALPDKAQAMRAADIPVAGTLDDLLRKVDVVADCTPKKIGAANKILYEQ
                     AGVRAIYQGGESHALTGHSFVAQANYETAIGRSSTRVVSCNTTSIVRTLGALKKAGLL
                     KRARGTLIRRASDPWEAHLDGIMNTVIPEKTIPSHQGPDAKTVDPDLDVVTIAAKASH
                     NSSHLHTWWVELTRSASKEELLAAFRLAPRIAFIRMHEGVAALNSTIELMSDLGRRRG
                     DLWEVALWEDVLTVNGNEAYYTYQVFNQAIVVPETIDAIRALTGLESQAERSIQKTDQ
                     ALGIAKEFLRSSEHVLMA"
     gene            complement(23106..24308)
                     /locus_tag="PK589_04885"
     CDS             complement(23106..24308)
                     /locus_tag="PK589_04885"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNJ18964.1"
                     /translation="MKRIISDLPHDRYRERTVLVRVDFNVPLDHGRIREDYRLRQAIP
                     TIEYLSQRGAKVILASHLGKPKGKVVPALSLKPVADRLGQILRVPTVKFVEAAVGESV
                     KQTLTTLKPGEVLLLENLRFEPGEEANDPVFAKQLAALAELYVNDAFGTMHRAHASTY
                     GAAQLFTDRLAGFLVRKEMDVLSQVRDRPVRPFRVVVGGIKIKDKLSALKALLPKADT
                     ILLGGGIAYTFLAAQGVATGDSPVEPEFIPWAKDVLAQYRKHILLPEDHVIAANAEQR
                     DRLQLVHREIPAGFMGLDIGRDTSLTFAHELLQGTGTIFWNGPLGAFEVDECADGTVD
                     IARAMALAHWRGAFTVVGGGDTVAAVRKAEVLETEISHVSTGGGASLKFIGGEELPGI
                     AVLSEQSQ"
     gene            complement(24331..26772)
                     /gene="ppsA"
                     /locus_tag="PK589_04890"
     CDS             complement(24331..26772)
                     /gene="ppsA"
                     /locus_tag="PK589_04890"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNJ18965.1"
                     /translation="MSYTATTTQAAIRWFEDIGIDDIPLVGGKNASLGEMYRELASRG
                     VKVPNGFAITAEAYRSFLRTTGLDQRITQIIRGLDTRDLDNLRQRGRQIRQAMLAASL
                     QQDLERAIVEAYDRLSDGHVDLVDVAVRSSATAEDLPDASFAGQQETYLNVQGHQALL
                     DACKRCFASLFTDRAISYRVDKGFDHFKIALSIGVQRMVRSDLSASGIMFSIDTETGF
                     RDAVLINASYGLGENVVQGAVNPDEYYVFKPTLRQGFRPILQKMLGSKEFKLVYDVGG
                     SKLVKNVPVPPDDRARFAITDDEILTLSRWACMIEDHYSAKKGRTSPMDMEWAKDGHT
                     GELFIVQARPETVQSQKRQDSLELYRLGRKGKVLLTGRSVGSKISLGPVRVVKHLQQL
                     RDFTSGDVLVTDKTDPDWEPVMKKASGIITNRGGRTCHAAIVSRELGVPAIVGTGNAT
                     EVLRDCQMVTLSCAEGDTGFVYEEALPFTVQRVELSDLTRPRTKIMMNVGNPEEAFAL
                     SFIPNDGVGLAREEFIISTYIKVHPLALLNYDRLDDAAVKAEIDRLTVGYADKAQFFV
                     DKLAQGVAMIAAAFYPKDVIVRLSDFKSNEYASLVGGRGYEPTEENPMIGFRGASRYY
                     DSRYQAGFALECRAMKKVRGEMGLTNVKLMIPFCRTVEEGRLVQAEMAKHGLTRGENG
                     LEIYVMCEVPSNVVLADQFAEVFDGFSIGSNDLTQLTLGVDRDSEIIAHLFDERNQAV
                     TSMMASVITTARKKGKKIGICGQAPSDYPEVASFLVEQGIDSISLNPDAVLKTTMAIL
                     KLEQGLQRGPAQRTG"
     gene            complement(26806..27288)
                     /locus_tag="PK589_04895"
     CDS             complement(26806..27288)
                     /locus_tag="PK589_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNJ18966.1"
                     /translation="MALAQRACSGTALVLCAVLVGPVHAAKVSDAAATHEWMSTPVRT
                     AAEQMLAAMPDDYYSIKGVAAEKAIATGVPHVLDVREPNEFAVEHIPGAKNVPLRSLM
                     TAIDDLPGDKAASLIVYCKTGHRGAIALAVLRMLGYANVLSLYGGLDGWKAAGFAIEK
                     "
     gene            complement(27374..29002)
                     /locus_tag="PK589_04900"
     CDS             complement(27374..29002)
                     /locus_tag="PK589_04900"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNJ18967.1"
                     /translation="MERTPFAITLDPNTSLANHTGNWRTMKPIYVDRLPPCNHACPAG
                     ENIQAWLYHAEEGAYEEAWQQLMQDNPMPAIHGRVCYHPCESACNRGHIDEAVSIHAV
                     ERFLGDQAIAQGWRVACAPRTGKNVLVIGAGPSGLSTAYHLARMGHQVTIHEAGPQAG
                     GMMRYGIPKYRLPREIVDAEVARIQSMGVTIRLNSRVDDLEATMAEGRFDAAFLALGA
                     HLARRVEIPGVDAKRVLDAVTFLRDMEGAARPKLGRRVAIYGGGNTAIDVARSVERLG
                     MEPVIIIYRRDLGHAPAFASEITEAQDEGVQFQWLRRIAQGDESTLTVEEMALDEQGK
                     PQPTGRFERIEADSVILALGQRADTGFLRRVSGLRVREDGTIEVDGTMMTGRPGIFAG
                     GDMVPGEQSVTVATGQGKKAARHIDAYLRGSAYVKPEAHEPATFSRLNTWYYTDADAS
                     KQPLLNLVRRRSSFDEIVGGLDADTALLEARRCLSCGNCFNCDNCYAVCPDSAVIKLG
                     PGKRYAINYDYCKGCGLCAEECPCGAIDMVREVK"
     gene            complement(29011..30000)
                     /locus_tag="PK589_04905"
     CDS             complement(29011..30000)
                     /locus_tag="PK589_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNJ18968.1"
                     /translation="MTTERIKFYQTGTFTAGNRLLDEATRTVQSRLDRTNSLNSGHRA
                     CQGCGEALGARYALDAAMRASHNQLIAVNATGCLEVFSSPYPESAWQIPWMHSLFGNA
                     PAVASGIAAALRAKRRDDVTVIAQGGDGATVDIGFGCLSGMFERNDDVLYLCYDNEAY
                     MNTGIQRSGATPPFARTNTTQAVGEHPGNPIGNGKNVPRIALAHGIPYVATASVADLH
                     DLERKVTKAIGVRGARYIHVHVPCPLGWGSDPAHTMKVARLAVESGLFPLFEAEFGKV
                     ICSTPIRRKVPVERYLELQTRFKHLFGPPQDTEALAAIQAMADDNIAVYGLME"
     gene            complement(30012..31265)
                     /locus_tag="PK589_04910"
     CDS             complement(30012..31265)
                     /locus_tag="PK589_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ18969.1"
                     /translation="MRMQIEGSQAVAEAIALCQPEVIAAYPISPQTHIVERLSRKTKQ
                     GHIGRCQFLNVESEFAALSVLIGASASGARTYTATSSQGLLFMAEAVYNVAGLGLPIV
                     MTIANRAIGAPINIWNDHSDSMALRDAGWIQLFAEDNQDAVDLHIQAFRLAESLSCPV
                     MVCMDGYVLTHAYEPVDVPDAVQVNAYLPPYDPVQVLDPERPVSIGAMVGPEAFTEVK
                     YFSHQQHLRALEVMPELVEVFEQHFQRRSGGLCSAYRMEDADIAVIAMGSVIGTIKDA
                     VDELRNTGVRVGCVKIGCYRPFPKFQVSALLEKTTRVIVVEKDLAVGMGGIVSADVQS
                     ALQGRQISVHTVIAGLGGRSISTSSLKHMILEARAERLTEPYFLDLNKTVIEQELARR
                     RDIRRAGPVAEHMLQTMNVIGTKVS"
     gene            complement(31249..31851)
                     /locus_tag="PK589_04915"
     CDS             complement(31249..31851)
                     /locus_tag="PK589_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNJ18970.1"
                     /translation="MWQIRIHGRGGQGVVTAAELLAMAAFMDGKYAQAFPSFGSERMG
                     APVVAFCRVAEGAIRTRETVTRPDVVIIQDPTLLHQVDLFGGLAPLGFVLINSTQTLD
                     ALHIEEFLSTLPLGHVCAFSATDLGLKYLKRPIANGPLLGAFAAITGLIRLASVESAI
                     QQKFPGAVGEANRAAAREAFDVVTTSITEQRGIVHHAHAD"
     gene            complement(32504..32752)
                     /locus_tag="PK589_04920"
     CDS             complement(32504..32752)
                     /locus_tag="PK589_04920"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNJ18971.1"
                     /translation="MKAQTTQPFREKKPHTMQVEQPNVPVSTRKAAGSAQAAVDDLHA
                     RITARAYELYVVRGCRNGYHLEDWLDAERVILDRAVTK"
     gene            complement(32966..33205)
                     /locus_tag="PK589_04925"
     CDS             complement(32966..33205)
                     /locus_tag="PK589_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18972.1"
                     /translation="MMSHHETDDRVLEVIVRSQGRTLDDIVLECPELTWNQVFIVIDR
                     LSREGAIRLMPKERGCYTVHLASNVFHDARQYARV"
     gene            complement(33434..33790)
                     /locus_tag="PK589_04930"
     CDS             complement(33434..33790)
                     /locus_tag="PK589_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18973.1"
                     /translation="MTTRIVMLLILCMLPATAQADASLSRAPWKERAVLRTGQVVQGD
                     YFAFGPHVEISGIVNGDVIVAGAKVILSGTVAQDARVAGAQVVSISLTHRHVVSRERC
                     SSASDMPVIQNHQQDS"
     gene            complement(33841..34266)
                     /locus_tag="PK589_04935"
     CDS             complement(33841..34266)
                     /locus_tag="PK589_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18974.1"
                     /translation="MHPIHPMVVHFPIALLLASTLFDALAFRWRRHQFRDTSLSLLVL
                     GILAAGAAVLTGHFAEEAVERSGIPKQAIEIHEELGGSVFWVFLGLLGLRLASCWGWM
                     REQPMLVLVIGLSGGLLLLIASYFGGDLVYRFGAGVLPR"
     gene            complement(34582..36273)
                     /locus_tag="PK589_04940"
     CDS             complement(34582..36273)
                     /locus_tag="PK589_04940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNJ18975.1"
                     /translation="MKREGEDGMEWWNPVARRCLEMTLLALVGGWAYFVNLHLSLLEG
                     SEGLYAGIAGEMGRRREFFDLTYHDVPYFNKPPLFFWLLNLSTSIWGDHEIALRLPGS
                     LAAVGTIILIYVLGVRLVSPAAGFWAALVVATSHVFLWYGRRVLFDSTLTFLVTLALF
                     AWIQVQLLGRSSRWYLLAFVSMALGAMLKEMHGFFLPLLVMALYAAIQRDTRMLKDGY
                     FWAGFALAVALMAGYAQMLGPGYQHHFRISNAIQSVWNTAFTRTSGPATDGHPLYWYL
                     GMMWADFFPWCAVLPSALLLLWAQRPFRAHPTELLLLVWVLSLFTAFSLATLKREPYL
                     TTIVPGIGLMIGYYYHRLFSSAEDSAAMTPLLRVLLGVLAVTFAAGMFFGAAPLRRRW
                     LVSTAVVSPMFIVTIVSLAGLLLYAVLRSRVRLALGMVGALAVAYVVLVVNYVFPAID
                     QAASPRKAVEEIKTLALGTQPALFMYIPGWPKNEDALYYLKRDNVVPDLPSQDAVREA
                     VRTHGAIRVVTEEQHAAALQSQAGLVVGKLQEFRQPGRKHLFLLSVQEQTSARVL"
     gene            37298..38722
                     /locus_tag="PK589_04945"
     CDS             37298..38722
                     /locus_tag="PK589_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNJ18976.1"
                     /translation="MKAGRWVAHTQPRLDHPDQWTREVAAEWVAAVLSMKKGDFCQTT
                     YLRQGQQGIPISPRHKMQLLSALNVFFRDCQEWEWIPRRFDPRRAFAVPRMLLALIHP
                     NPKPLPDDLWAKALSAGLQLGADDLASLSPRWPTRRFYPIEMVRAVAMVWLFSGLRSD
                     EIVRLRVGCATTKVLDEQRGKTVCLLEVPTNKTSRSFVKPVDPIVAAVIDAWEQVRPQ
                     QARFIDRKTGEPVPLLFAFRGRPLAPPYLNHTVIPLICTKAGIPRHDRHGTITSHRAR
                     STMATQLLNSPDHPMTVFELQQWLGHRSVSSTLHYLAITPTRLSESFLKSNHTHRNLR
                     TVQVLIDQDVVLSGDAGTQPWKFYDLGHGYCRYDFFEHCPHRMACAKCSFYLPKGSTE
                     AQLLEGKINLLKMRQEIPLQPAELAAVDEGIEAFERLLTQLRQTPTPAGLTPLQIDDA
                     KTSARESGVQAGLPETSAARPTVT"
     gene            38923..39495
                     /locus_tag="PK589_04950"
     CDS             38923..39495
                     /locus_tag="PK589_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNJ18977.1"
                     /translation="MNPNFRNADAFTKLVLQPYRDAQFGPNTGPFLYEFQRHGLPAEE
                     FIGKLDDFFGSVSTDFRYAVEIRNAGLLGPRYHDMLRAHGVAHVYNHWSYMPPLAEQH
                     QRMKTMTAPFTVLRLLTPLKMTYEAAKKRAEPYDKIVGELPEMRKDTVSLIQEATAEN
                     RSTYVLVNNRAEGNAPLTIQALIDRLQTGG"
     gene            complement(39519..40001)
                     /locus_tag="PK589_04955"
     CDS             complement(39519..40001)
                     /locus_tag="PK589_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18978.1"
                     /translation="MLNIHRIDHEASHTHCWRVTVQRRTRIYVRSFSDGRYGGAEQAL
                     VAATTYRDALIRSNPPLSKPVYCAILKKTNRSGISGLSCIDRWEFSKGRRVHRLYWDV
                     QWPIENCQSRHKKFSILKYGEDGAYRRALKARAAALKAISSQTFSPFASRVAANSAAA
                     "
     gene            complement(39995..40279)
                     /locus_tag="PK589_04960"
     CDS             complement(39995..40279)
                     /locus_tag="PK589_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18979.1"
                     /translation="MFHSAQDNLYDVGKIVGLSMHHCHVESQAEVTPGMTLSLFVVLP
                     TKRRALVLDEALVTWVRTEEFGVRLKTIPGEDAAYLESYLAGHKVDAGLC"
     gene            complement(40468..40710)
                     /locus_tag="PK589_04965"
     CDS             complement(40468..40710)
                     /locus_tag="PK589_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18980.1"
                     /translation="MRWADQYNVMRSMLLSSPATHWWVRDLTLRLDAKDPVDVLNGLE
                     VVARLMRMRHEAVRAGEWDQLECQKPTGEDGISMKD"
     gene            41221..42882
                     /locus_tag="PK589_04970"
     CDS             41221..42882
                     /locus_tag="PK589_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18981.1"
                     /translation="MPAGIRTSLTRVYTYPAYLTFSSYAALPAAVREEADQMQSLWNA
                     MVDVYEVAHRQQERLHKQFLQTSRTTTRAAGGTRSIGDPFECIKQLRDQNDSLEDETL
                     LALSRTYRTAAAEYEASEHHNIDRCLPYPESMKQELSQITKRLHDTLHQLARTSPLSR
                     SHSNAVLERMKTAIRRVPVGNLPPSKETGPPTSLFFTHDFGSQGIRIQSLTRADVTQS
                     GSSFAANRLLTLHPVSQFAEDTDPRERGQRDFCRTEGELFVRNVPIPFEIILTPLLPK
                     DALLKQVSLIGSQQRPQTSVNMDSSNACLRNDTTWQWYLQFTLIVPPTQQSEPKKYGM
                     IALDFDHRLINDANPLVRVGEWVSDTGKHESVFFPARVVKNLIAAYEAREALNVQHLA
                     LKQLFVHFPFAGALADMVKEPIHSDVPLSRKRLRWIAHQAVKQAYSGPLTSIIREILS
                     QIRDTRIDVVRRRRQWRRDRAEFYDKLAHRLSREAGTIVLTPVLSAGRSGAEGSSTST
                     TRSAPLAQALAHPINMQEFIHRLQHAAPIHRTTLHFVQSMHALIA"
     gene            43168..43701
                     /locus_tag="PK589_04975"
     CDS             43168..43701
                     /locus_tag="PK589_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18982.1"
                     /translation="MKTEAVPAPPPPRLEEEDDEDEQDPIASFEQDSNDFQRATISIV
                     MTLLPTDHHPDGRLTLIGVRSHNLPPQLTTARFNDLLPLPESLSQALASWEQTFTAAL
                     TARKTQRDAKAAEQQAKTVKQEKKREAERQARKPKVAKPAAAKAPPVALTPKPDREQH
                     THDSTSNTALPQAGLFE"
     gene            43715..44152
                     /locus_tag="PK589_04980"
     CDS             43715..44152
                     /locus_tag="PK589_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18983.1"
                     /translation="MVTLQLEGQEIQLPPEIAISDDAVRKAIAPLFPQAAHATIQRTT
                     DTNGMTTIRLTKQAGTKGNYHVILRQLIESPRHVNPALTLYQAYQAESQPTQTTVESI
                     LLTQQKLRLAIEHGEKEYKETATIKERLRNTPGSPSRLLPPGF"
     gene            44948..45631
                     /locus_tag="PK589_04985"
     CDS             44948..45631
                     /locus_tag="PK589_04985"
                     /inference="COORDINATES: protein motif:HMM:NF025815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18984.1"
                     /translation="MRGASSDQHDASLHFIGGQLLLEYKDGEAVIRKCISPEAARNAF
                     SSAGIDSDWLPEHVCRYGIGPRGPWLLLRFAPGRYQIPLADPITLSGGGEPHTMLAVP
                     MPGLLFLGYGTHYYVWAYKLWKCAATKLFKAPLANVYPDGAICFGNVHPRVAHGNTIT
                     NSWRLFWDSNFSDHLANGKSNAHPDNILPFLAKLHSTEAQDYPLDDLEPAHLSIATII
                     RQLMQSGKG"
     gene            46351..47178
                     /locus_tag="PK589_04990"
     CDS             46351..47178
                     /locus_tag="PK589_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNJ18985.1"
                     /translation="MKVLPKQCPRLHVIQIGCGGIGAFLGIHVARLARECFRLFDTVM
                     VTFYDGDTIEEKNLRRQNFCQAEIGHNKAEALAFRLSTAWGIPIRACPRHFSGKEAHP
                     EYDTLTILLGCVDTARARRSLHHALARWNTRNDAQAWLIDGGNLTTHGQVLVGNVVTD
                     FNPHESFQLRTVCQSLPAPALVHPELLKRERRPVNTQQRSCAELALGDPQSLTINSII
                     ASHMADYLLRLVITKDLRRFATYIDMDTGSARSLAITSTEISRVLGLPHTESMGRAS"
     gene            complement(48689..48829)
                     /locus_tag="PK589_04995"
                     /pseudo
     CDS             complement(48689..48829)
                     /locus_tag="PK589_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012736482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(48938..50064)
                     /locus_tag="PK589_05000"
     CDS             complement(join(48938..49809,49809..50064))
                     /locus_tag="PK589_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNJ18986.1"
                     /translation="MKRTRRNHGAAFKAQVAVAAIKGDKTLAELAEQFSVHPTQITEW
                     KQQLLARAADVFGGTTPTSDTPDLKVLHAKIGQLALENDFLGRGAHQGGIVERKTMIN
                     RTHALPVVRQCQILGLSRSTAYYQPTPVSGTELALMRRLDELHLRHPFAGARMLRDLL
                     RQEGQAIGRRHVATLMRRMGLEALYRKPRTSHRHPAHPIYPYRLRDLTISRPNHVWAA
                     DITYIPMAQGFVYLFAVLDWASRRVLAWRLSNTLTTDFCLEAVQDALAHYGTPEIFNT
                     DQGCQFTSQEFTGLLKDHGIQISMDGKGCWRDNVFVERLWKSIKYEEVYLHAYETISA
                     AQQGVARYLTFYNQTRPHQALDGQTPDQVYYDHLTTRRTAA"
     gene            50324..53515
                     /gene="dnaE"
                     /locus_tag="PK589_05005"
     CDS             50324..53515
                     /gene="dnaE"
                     /locus_tag="PK589_05005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNJ18987.1"
                     /translation="MPTPFVHLHTHTTYSLLDGATLLDPLIEHVRWLNQPAVAITDHG
                     NLFGTISFYTKARSAGVKPIIGCEVYMARGSLLDKQPPEGPRDFYHLILLAQNSIGYH
                     NLIRLVSHGYTEGFYYKPRIDKSLLRSHSSGLIALSGCLTGEIPTLLQQEQFASASVA
                     AQEFLDIFGPDRFYLELQANGLHQQQRVNQGLIHLHRELGIPLVATNDCHYLKRGDAD
                     AHDLLLCIQTGTTITDTTRLRFGTDQLYVKSSEEMAEAFRELPQAITHTNLIAEQCTL
                     EIPLHRTQLPRYDVPPPHTLDSFLETLAQQGLSARLAHHSGPLDRPTYERRLRTELLT
                     ICSMGFAGYFLIVWDIMKFARSRRIPVGPGRGSAAGSLVAYALGITELDPLAHDLLFE
                     RFLNPDRVSLPDIDMDFCMKRRQEVINYVTEKYGRDHVAQIITFGTLGAKAAIRDVGR
                     VLEIPYDEVDTIAKLIPSQPNMTIAQALAEEPRLSALSASHPSVGTLLSVAESLEGLA
                     RHASTHAAGLVISNTPLLETVPLCRTTSDDIVTQFGMGELEQLGLVKFDFLGLKTLTV
                     IDHTISLINQQSLGSRPLTLDDIPLDDAKTFALLASGRTTGVFQLESNGMRELLREFQ
                     PDRFEDITAIIALYRPGPLDLIPEFIQQKRGQYPLASNIAALEPILSTTYGVIVYQEQ
                     VMAIANTVAGFSLGQADILRRAMGKKKPEEMRALHAQFVEGAVGLGTEREAAEHLFQM
                     ITKFAGYGFNKSHAAAYALLTYRTAYLKAHDPLAYMTALMTSDIGDHERLSLYASECR
                     EMGVRLLPPDINRSETGFTIVDGAMLYGLAAIKHVGEYLATQLIQIRATGGTFASFFD
                     LCDRVDRRTVTKRVLDALIKSGALDTLCDHRAQLSAILDDATPAKPTRSQRNVTHQTL
                     LQLFPEESETRVLPKIPAWSPSIRLDHEREALGCYVSHHPVRVHDHQLKRLQVVPLTQ
                     LPDLPDGKEVRIAGLITRIKRILTKRQEPMAFVHLEDQDRTLEVVVFPSLYRHDSGRL
                     EPGTVIALEGILDQNDQGIKVKATRFLPLDPAA"
     gene            53720..54298
                     /locus_tag="PK589_05010"
     CDS             53720..54298
                     /locus_tag="PK589_05010"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ18988.1"
                     /translation="MTLGCSLGMTLLGAGVVLAQPVDLPAVARCQMEADSQPNRQTLI
                     RNWIHRAGRTDALAALCLSFSPNPNAGESLAWLRRAADLGLPDAQLLLGADLEVGFKS
                     PPNSQEAMRWYRKAAQADFPFAQYELGAIYSEGRANQRQDLPEAYYWAQLAARHQFRP
                     AKELADTLSPQLSHDERARIDARIKKWKRVVS"
     gene            54339..54557
                     /locus_tag="PK589_05015"
     CDS             54339..54557
                     /locus_tag="PK589_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18989.1"
                     /translation="MGPLATSLRQIISTIIRYLPGGASAPRLAHEEARAQQERVDLAM
                     LQILVQESERPHTDVSLSASPLKDDPHP"
     gene            55434..55625
                     /locus_tag="PK589_05020"
     CDS             55434..55625
                     /locus_tag="PK589_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18990.1"
                     /translation="MAFIEQHGRKIYQFSDNPPRPRHQYQILSGIPLPDIPRYQETPL
                     EYHARTGYPLDLCRRYLNP"
     gene            57107..57325
                     /locus_tag="PK589_05025"
     CDS             57107..57325
                     /locus_tag="PK589_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18991.1"
                     /translation="MKKDYAVSVIFPEVLVKHSRQHIRISASNLRLAANEALKAIMKR
                     EGIKGRRLTRLQLAVIHAGTSTIQDHEA"
     gene            57930..59363
                     /gene="xseA"
                     /locus_tag="PK589_05030"
     CDS             57930..59363
                     /gene="xseA"
                     /locus_tag="PK589_05030"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNJ18992.1"
                     /translation="MTMSLPSTHSTETPQPILSIGMLSQRIRRQLAPMLTKQTYLLQA
                     QWIKPQANKGQKGFFSVTLADTDNPNMAIDAMIWEPAVIRKVLEQGQQFGHDLLSRDS
                     RVEVVVEATLGFWANKNTIYVLIHQLSTIGMLGLRQQQREAALRTLKEEGLLTRNASL
                     PWPRFPLRIGIIGKQGSDAVHDILNVLHSSPYRFETTIFHTAVQGVGAIPGLLKAFHD
                     VAAHTTPLDVIVMSRGGGNEIDLVAFDNLDVARAVATASKPVLTGLGHHLDRSICDEV
                     AAKALSTPTAAGQYLVSHLDRIRECLFTTHATLHAHTRHTLLEWTHALTLTRHALWER
                     ARNTIEAHATASHHAHERFQRALRDRLEASYQQGKTLRYSLTASLTQALSQLSMGYAF
                     TLVRLHTAGLSRVDTLKGAITAFVQALTLSDPQHLLARGYAYVLDNQGRMVTSHRTVI
                     AGAQLHIHVQDGRITATTLHTEPSHEQ"
     gene            59353..59589
                     /locus_tag="PK589_05035"
     CDS             59353..59589
                     /locus_tag="PK589_05035"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNJ18993.1"
                     /translation="MSNEDTSHPQLPTSYDAAFKELEDLSARATKGDINIDELSTAIQ
                     RGNQLFAFLSSGIAKIKAEIEDHAQDGITQSKPN"
     gene            59650..59883
                     /locus_tag="PK589_05040"
     CDS             59650..59883
                     /locus_tag="PK589_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18994.1"
                     /translation="MSKHHHTHPITIDQTLDLLAKQWTAADRIRLRTARHRPRHVRAA
                     ELWTRIQSLTQSTLALFQHPSAMETSHGHHSDR"
     gene            59861..60238
                     /locus_tag="PK589_05045"
     CDS             59861..60238
                     /locus_tag="PK589_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ18995.1"
                     /translation="MAIILIVDDYSDIRACLRTMLEEAGHIIVEAGSGQEALHAWNET
                     RPDLVITDHSMPGMSGVELIRALGQRQPEVKGILMSADQDPRPAEPPDQSHAWPFLQK
                     PFGFRALRQAIDSLLLAQPAEPA"
     gene            60349..61278
                     /locus_tag="PK589_05050"
     CDS             60349..61278
                     /locus_tag="PK589_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18996.1"
                     /translation="MADSDFLSYAQLCQFVREQGRLPRLDDPIPAYRYAGWALPMIWE
                     GHRVLPGVPNRWGYHLDILHARRIPDAPIPQIEFLHTPHRDTLTHLQRWVTLAAEHQS
                     SWTGLTNLIAWIAFSLKLNHVPPSLADNTQAALYQAVNLQELVLHPYDYWGDIIAEGI
                     GRGPWANPNGFYPTPMSVCQLIVQMMLPDADSLSPAARNALRVKSIADPAGSGTGRML
                     LLASNVSLSLHGIERDALVRTACLINGALYAPWLAFPLPQEILHGTDAEHGLTNQASL
                     LSVATPIPEEISLIPPINDAVDHEGLQLVFPGW"
     gene            61383..61826
                     /locus_tag="PK589_05055"
     CDS             61383..61826
                     /locus_tag="PK589_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18997.1"
                     /translation="MRVLEAHGDLWDVHAQGSWIAITTNGVIRTNGHGVMGAGLAKQA
                     ADRFPSLPSLLGTHLRRFGNIPTSIPSMRIMTLPTKHHFRTASDLALIQNSLQHIQLI
                     LTRERIDRLYCPHPGCGLGQLSWQQVRAAIVHVVDHRFLFLHSTA"
     gene            63005..63505
                     /locus_tag="PK589_05060"
     CDS             63005..63505
                     /locus_tag="PK589_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HNJ18998.1"
                     /translation="MKTALDQHQLGIYDVPRYTISLIKEAASTFHAKTRCQPSEDVYR
                     YFKDICENQDREHLYALFLDTKHVPIGMNHVAMGTLSTTIVHPREVFKCAILLNAACL
                     VIVHNHPSGDPSPSPEDRALTTRIAEAGQLLGIALLDHVVIGNGRYFSFADHGPLTQQ
                     TLTYTS"
     gene            64279..>64471
                     /locus_tag="PK589_05065"
     CDS             64279..>64471
                     /locus_tag="PK589_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ18999.1"
                     /translation="MTPSIRCPFRRRSIPRHHPFWPPNSNQIDSGIRTIYDLSKGIAL
                     FGVFVPLVTFNGVWYKLVLA"
BASE COUNT        13506 a        19936 c        17478 g        13551 t
ORIGIN      
        1 aacaccgtca tgtagacatc ggtcggatcg atgagcgtca ggacctttcc tcctgctgcc
       61 agcacttcgc cgggctccgc caatcggaac tgtacccggc cgctccgagg tgcagtcaat
      121 acgctctcct tgaggtccgc ctgaatccgt tccgattggg caaggctcgc cttgatggcc
      181 gcttccgcct cggccaattg ggcctcggcc tgggtgatcg cggacttcgc cgtgtccatc
      241 gtggaccgcg cgcgatcgta gtctttcacg gagatattgg cgttgacgta gagagcacga
      301 gcccgttcca aatcccgttc ggctaataag gtttcactcc gccgttgagc gatcacggcc
      361 ttggccgtga tcttgccttg cttggctcgc tggacttgcg cctccgcttc tcgcacctgt
      421 gcgttcaacg aactcgtatc catgcgggcg acgacttgtc cgacttggac gctatcgccc
      481 tcatcaacca gcacctccgc aatgcggcct gggaatttgg tggccacatc gatctcctgc
      541 acctcaagcc gtccgttgcc gctgccgaat cccacgagcg gtgggattcg aaaatagaac
      601 caccagacgg aggctcctgc cacgagcaac agaagcccaa ctcctagtat gagagtgggg
      661 cgagcgatct ttggggtcgg catggagtca ctccgtgtca atcagttccg tgggtcatcg
      721 cgcagaaccg gcgagacgcc ggcattcaca aaatgcgttt tcagtcgttc gaaccaggcg
      781 gctcgctctt cggagcttcg aaagaaactc atctgcccca ccgcccgctg taccttcgcc
      841 agatcgatca gaaattcata gaccgcattg gcggcggcct gattcgcggt cagcgcggca
      901 ttctgcgaat tcagaagctt gatgatgtcc accgcccctc gggaatattg atccacgacc
      961 agatccaggg tcttgcgggc cgactcggcc gctttccgcg agagtctgat gctgggaaag
     1021 gaagcgccgg tttgatacag cgtagtccgg acccgctctt caacctgaag tgcgacggcg
     1081 gcccgctcga actgcacctg acgcagttct tcacgggcct tgagggccgt ggcatctttg
     1141 tatccgccct ggaacagggg gaacgtcaac ccgatactcg ctacataaaa attgttctgg
     1201 gtctgcgcaa agccgaccgg tccaaggggg accgtgaggc ttcccgctcc tcctccccct
     1261 tgggcatatt gctgaaaccc ggcaccctgt atggcaactt ctgggagcca ataggctcgc
     1321 tgagcattcg tgatggttcg ctcctgagcc gcactcaagg cgtcaagccg tcgcaactcg
     1381 ggagccgcct ggatcccttc ttcgacttcg aagtcccgga atacttgaaa tccccgcgga
     1441 ttctcgatat acgcgaacag ctgacgcgta tcgcccagca atcccgggtc ctccagggtt
     1501 tgctccacgg tttgaaacct ctcttccaga ggacgattca agattcggtt caggtccact
     1561 tccgcctgcc gttcttgagc ctcggcggaa accacggctt tctgcccgtt agccacctct
     1621 gcttcccatc gaaacagctc atcccggagg gcatagccga ccgactcacg ggccgcagcc
     1681 atgtctagat tggcacgcgt cagtttgagg ttatccttct gaatacgtcg cacggtcttg
     1741 gtccgaagga cggtcagata ggcgagggct gcgtcctgtg cgatatccag ccgcagctgg
     1801 ttccactcct cttctcgaga cagttgcgtc ttctgctgca ccgtgtactt ggtccaggcg
     1861 tcgtccgagt acaggagctg gcggaatccg ccaagggcaa tcgcggcttg accgggggtc
     1921 ccgccggtgc caaacgtatc cggccctcgc ccgttcagaa cccctgacgt gccgacgagc
     1981 acctgaggca gaagaccgga tcgtgcctgg cccacctggc cgactcccgc cgcgaccttg
     2041 cggctcgccg cggccagatc caggttcgct tctgcggatt cacgaacggc ttcgaagagc
     2101 gacagacgtc gtgaggtgcc ggctgcatcc tcgttcacca actctgcctc gctcagcact
     2161 tggaagctcg gccacttgtc gatcgcacgg actgtcgcca tgttgatcac gagctgctcg
     2221 ccccggataa aggccacttc aagccgtccg gcgtcggtcc ctgacagaat acgctgcaca
     2281 ttcgccgcga tcgttcgcgc gaatcgcccc agatccaatt caggcgacag agcgagagtg
     2341 atcccgcgct gtacgtcccg ctgtcccact gccgaaaagc tgggaagttt tcgccggttg
     2401 agagccaaaa ccagacgatc gaattcaccg gcgagcagcc gcgggagagg cgagagatag
     2461 acggcctctg tgtcgggagg gatcgagcca atggccgcct cggcagaggt ctcgaccgga
     2521 accacggtaa gcttcacgcc gtacgtctca gccatctttc gcgcgcggtc cttaactggc
     2581 ggaaaggcct ccagtaacac ccgatctgcc aagagggtta accgggtaaa gggaaacacc
     2641 tctttgaagg cctcgagatc acgctcgaag ccctttccgg tgacgaggta actgaggttt
     2701 ttcatcccgc tcgtgccctt cttgaacggc agcccctgca agtgcggatc gaggatcaac
     2761 ggcgcgatca caggcttggg tagttgcctt ctttgactga cctcctggga agcgagaatc
     2821 ccgagtgcga tgacgagatt gacacggggc tgagccagca cacgatcgat cgcctgtttc
     2881 accccttgca ccgaccatcc tccatcaaac tgcaaatcat tggggaaccg cacctcgaat
     2941 tcccctctgg tgatctcgag aatttccttc tgaatgagat cacggagctc gggaacaagc
     3001 tccgagggtc cgtcgcgcac gatcccgacc tggaccaccg gtaacgactt ggcctccacg
     3061 ttagatctcc aggacagaag gaagatgacg ctgatgatgc aaaagaacgt tttggtatgc
     3121 atgagccatc actccccgac aaggttactt ctccgtggcg atgcaattct ggactactgc
     3181 ctccgtaagt gtttcttcct gctgcaccat agatcaaaac aacctgacac gtctagccca
     3241 atctcttgcc tccaacatat tatgagcagg gacaactata gagcctcctt tcacgcagac
     3301 gtgatgagcg gcttcaacat gccgtctgct ttcgttctca tcagctcaaa ggccttctcc
     3361 acatcgttgc gaaatgtaca gggcatcggc ttcagcgatc ctgcacagtt cctctcgagg
     3421 gtggtggcga cgccgagccg gtcctggcgg aaggagatgt cgcgaatcac gccctttttg
     3481 tacgactccc agatgtggcg cgcctcatcg tgcaggtgag gctgctgttt ttccagcgtc
     3541 gcaccgggtc gggacggtcg aggcaaagaa tcttcatgag cgttcctttc gggcaagcat
     3601 gttccttcta ccgtccatgt ttcgaactac ggccgcagca gcgccttgat cgcgcggcgc
     3661 tcgtccatcg cccgatagcc ttcagccacc tgatcgagcg gaagagtcag atcgaacacc
     3721 ttgcctggat tgattttcct gtccagcacc aagtcgatca gcttgggcag gtagcgacga
     3781 accggcgcag ggccaccgtg gaggtggaca tgtgagtaga acaacttctc accatcgagc
     3841 aggactccgt gaggcacgcc gacgtaaccc acggagccac ccggacgggt cgcaagaatc
     3901 gcttgcatca ttgattcttg agtgccgacg cactccagca ccgagtcagc accaacaccg
     3961 ttggttagct ctttgatttg ggtagtgccc tcgtcaccgc gctcagtcac aatgtcggtt
     4021 gccccaaact cgcgggcgag ctgctgtcgc ttctcgtgac ggctcattgc gatgatccgc
     4081 tcggccccca tctgcttagc cgaaagcacg cccaggagcc caaccgcgcc atcaccgacg
     4141 accacgacgg ttgatcccgg tgtcacgttc gctgcatcgg cagcgaacca gccggtgccc
     4201 agaacatccg atgctgtcag taggctcggg agcaggtcgt tcgacggcac gcccggcgtc
     4261 ggcaccagcg ttccgtccgc cagcggcacg cggagcaccg gcgcttgcgc ccgaagcatc
     4321 cactcgcgat gcacgcatga cgactggtag ccggcctgac agtggggaca ggtgttgtcg
     4381 gacgtagcga acgagccgat gacaaactgc cccggcttga ccgaccgcac cgcgccgccg
     4441 atctcctcga cgatgccaca gtattcgtgg cccatcggcg tgggttcggt gatcggctgc
     4501 agcccgcgat acggccacaa gtccgacccg cacacgcagg tgagcgcgat tcggataatg
     4561 gcatccgtcg gctcgatgat ctttggcgcg tcgcgctctt cgaagcgcac atcgcgcggg
     4621 ccgtagagaa ctgctcctcg catggtgtac ctcctcgtct aaatggtgcc cgccgcgatt
     4681 cgtggcgggt gtacgtcctg cttctcgcgt cgagggcggc ccgctttcgg cagaggtccg
     4741 tctaccgagc cgccgagaat gcccaatccg gctccttccc ttcattctgg aaggatggcg
     4801 gtcacgcgga tctcgacccg catcttcgga tctccaaggg cagcgactcc caggcaagtc
     4861 caaactggag cgtggccggg catgtagtga cggaaccgct ccgtcatggc ctggttgatt
     4921 tcttcctggt cgccgtcgag ccccacgtgg taagagttca catgaatcac gtactcccag
     4981 cgcgcgccgg cgacagccaa agtccgctcg acgttgcgaa acgcctgagc gatctcatcc
     5041 gtgatcgact cggggaactc ccagtcatcg ttccagccgc cctgacccga aagctccagc
     5101 cggttgccga tcttgaccgc ctgggaatag tggaacagtt ttaggttgcg ctcaccgtaa
     5161 ccgggggtga cgaagaattc aggcttgttc atggcgttcg ctccctgtgc tggttgtaag
     5221 ttcaccaggt ttgcagctcc acactcgcta cagcagtcgc tgcggaaata ataaagtcac
     5281 gtcgtgagtg acctacgggt cttgattgtt aggttctatc ttcgtcgttg gtttctttca
     5341 tcattttctc cctacgcagc ccgacggttc agaaacgtgc ggcgtggtgc atagccatgg
     5401 gtgacagctt cgggattaat cgggtgagac cagtttttgt tgctccgccg gataacggtc
     5461 gcccacgatc tcaatcttcg aaacggaacc ctcgatctca ctcatttcgg cggccgacaa
     5521 tttcaggtcc gccgttctca ggttttcttc caggtgtgcc agcttggtag tgccgggaat
     5581 gggcacgatc cagggttgtt tggcaagcag ccaggccaac gcgacctgtg cggatgtcac
     5641 tccccgagtg cggccgaatc gattgagcgc ctccacgatc gccagattgg ctctcatcgc
     5701 ttccggcgtg aagcggggca gtgtcttgcg gttgtcgctt ccggaatcga accgggtgta
     5761 ttcattcagg ttgcctccga gaaagccgcg gttcacgggg ctgtacggga caaacccgat
     5821 tccaagctct ccacacaccg gcagaacgtc cttctcgggt gctctccaca ttagatggta
     5881 ttcgctctgg accgcggtga ccggctgaac cgcgtgtgcc cggcggatcg tcttcgcccc
     5941 ggcttcggag aggccgaagt gtttgacctt gccctcttgg atcagctcct tcaccgctcc
     6001 cgccacgtcc tcaatcggca cgtccggatc gacccggtgc tggtagaaca ggtcaatggc
     6061 atcgaccttc agccgcttga gcgaggcctc ggcgacctcc ttgatgtgtt gcggacgact
     6121 gtccaggccg atcgacgtgc gactgcccgg atcgatcttc cacccgaact tggtggtgat
     6181 cacgacttgc ccgcggaacg gggccagggc ctcacccacg agttcttcgt tgacgaacgg
     6241 cccatacacc tcggcagtat caaagagggt cacgccacgt tccgtggcct ggcgggccag
     6301 cttgatcatc gcatcccggt ccggatggag gccgcgatgg tagttcattc ccatgcaccc
     6361 aaagccgagg gccgacactt gcaatgcgtg ctttcctgag cccaatgtgc ggcgttgagt
     6421 gatcaaagga gagctggtgc ggttctgatc gcagatcggg gctggtccgg ccgccggttt
     6481 tcccaaggca ggtgttatac acatggcggc tcccagcgcg ccccccgaca gcaagaaacc
     6541 gcggcggctg attggagccg ggttgctttg gctcgcgttg ttctgtggat tcttttgttt
     6601 catttcagtt tccttgattg gtcgatctga ccggttcttg ttcgtcattg tgtggtgaat
     6661 ccggaagaac ctctcagttg atcggcccct ctaccgtgta tcggagccag acgctgccat
     6721 catccagagg ttgcacttcc ttcagcttga aggcggttgg gagtggctgg gtgtagcgct
     6781 catccgcctc gaacagggtc tgcgctccct tcgttccatc cgcagccggc gtcaggacga
     6841 tgctcacctc gtcgcacagg tctgcccgga tgaaagacca gttcaggcct cctccgccac
     6901 aaagcatgat ctcgtcaaca ccgaaggcgg agccgatctt ctttacagcc tcttccagat
     6961 ctagagcgtc ctcgccggcg atgtgtagga aacgcctttg cggcgcagga agtctttgta
     7021 ggcgttggtg gcttttccgg aaaggatctg aaccacatgg gcggtggtgt cgaagtaggt
     7081 gattgagttc gtttcccagg cgagcacgcc ggacgggtcc actgagaaat aatgcatcgg
     7141 cgcgtcgtcg acggcgatga aatcgccttc cggcattggc ggagcgaatt catccagctt
     7201 cagcttccga tagtgcgtga agttgtcctc gctggtagtg cggccagaca gccatccctt
     7261 gtgattgcgg aaatggcggt cgggcccaag gaagaggttg tagtactgcc tttgggaagc
     7321 catcccaacg tccgtgggca gataggaccc cacgattttt ccgtcaagtg acatgtgcat
     7381 atggatgaaa atacggggtc ggtccattgg ggttctccct agggttcgag ttcagtcgct
     7441 gacgcgagaa gtgacggtca gcgatgggcg gccgagcgga tggtctgatc cgcccgcgac
     7501 gggaagacct ggtgccgggt ttccatcccg caagtcgcgg ttgcgctgac cggcgccgaa
     7561 cgcgccataa ggatagccgt tcgatctggg cgcactgacc acttcgaggg ccttcgtggc
     7621 gttctcgtcc agtgtgagtt tggccgcagc aagattctcg tcaagctgcc gcaggttgcg
     7681 cgcgccgaag atcggtgcgg cgacgcccgg acgattggcg atccagctca gcgcgacctg
     7741 agccggggtg gcgccgatct ctttcgcgat gcggatgacg gtttcgatcg tcgcccaatt
     7801 ttgatcggag ccggcataat ccgcagagat ccactggtac aggtctttgc cggagccggc
     7861 acggctatcg gacgccgggg ttcctccacg ttgatacttg ccggtcagga aacctccggc
     7921 cagcggcgac cagggcagta ggctcaagcc gttatgccgc gcggccgggt tcatctccca
     7981 ttcgcactcg cgcgacagca ggctgtattg ctgttgcaga ctgaccagcg tctgcacccc
     8041 cgtcgccttg gcggtggaca ccatgagctg aagctgccag ccggtgaagt ttgacaggcc
     8101 cacgtagtgg atcttgcctg cgcggacggc atcatcgagg aacgatagcg tctcctcgac
     8161 cggcgtgtgc atgtcggacg catgcagctg atacaggtcg atcgtctcca cgtcgaggcg
     8221 gcgcagcgag gcatccagcg cccggtggag atggcggcgc gacaggccta ccgcattggg
     8281 atcggaatca tcaccgaacc ggcccttggt cgccactatg acgcgatcgg ttatctcgcg
     8341 aggacgtgcc gccagccaac ggccgacgat ctgctcggcg acggtaccga cgtagacatc
     8401 ggatgtatcg accagatttc caccggcatc gaggaaggcg tcgagaatgg cgaacgcatc
     8461 gttctcggcc gtctcatcgc caaaatacat tgtgccgagc gccagggtgg atacgacggt
     8521 gccggtattg ccgagcttgc gatatttcat gatgtgggtt cctttcgttt gatggcgcgc
     8581 gttgcgcaga ccccgatgac ggttatcaat gaccgagatg cgtgtgaccc tgcccgcaca
     8641 gcaggattgt cgtgaatgct tctttaacgg ttgatcatct tctgcgccgc ctcggagtat
     8701 cgttccccgt ggacggcaat gtttgccgcg gcgtccgcga tatcgcggag gtcatccggg
     8761 gtcagttcga cggcagcccc tccgacgttc tcttcgagcc ggtgcagttt ggtggtgccg
     8821 gggatcggga cgatccacgg cttctgggag agcagccacg cgatcgccac ctgggccggt
     8881 gtcgccttct tccgctcggc gatgcggccg agcagctcga ccatcgcctg gttcgccttg
     8941 cggttctccg ccgtgaaccg ggggacggtg tttcggaagt cggtgctgtc gaacgtcgtc
     9001 gtctcgtcga tcttcccggt gaggtatccc ttgccgagcg ggctgaacgg cacgaacccg
     9061 atgctcagtt cctcaagcgt aggcagcaat tcctgttccg gctcgcgcca ccacagcgag
     9121 tactcgctct gcagggccgc gaccgtgcca aggagaagca gggctgcggc aagaagctcc
     9181 ggactcgcat gcctgcgctg ccgtgccagc aattgcccgg cgcttgttgc agaacttgtc
     9241 attggcgtgc tggcactcac agggggtatt ccttcagaga ttggcccgga agaacggcgt
     9301 gatcttcccg agcacggctt cacggccgga tccgtcatac agacccatgt gcgaagccct
     9361 ttcgatgatg aagagttcct tgctgctgcc tggtgccttc gcgtagagtt cctggctttg
     9421 ccacagcgaa tcggcttcgc tgccggcgca gatcatgagg ggttgggtca gcagcggatc
     9481 gaccaggtcg aaggcatcga acgcgatcca gcccatgagc gaggtcatga gcatcttatt
     9541 gggcgcgttc ggatgccgac cacgtggcgt caggtaatag tcggagccct cgcgcagatc
     9601 gcggaaggga ctgctttcac cgacgttagg tacgtagggc gcgtaggcta tcgcggcccc
     9661 ggcggcttcc gccgtgcgct ggttgacgag catttcgagc gtgcccggca aagcggacat
     9721 agggctcttg ccatcccatc ccttgcgtgc cgcgttgccc acattcgccg cgctggcggt
     9781 ggcaaccgcc ttgatgcggc gatccagcga ggccgccttg acggaatagc caccaccggc
     9841 gcagatgccg agaataccga tgcgctcggc atcgacgaag ggcaaggtgg tgaggtaatc
     9901 caccgcgctg tagatgtcgc ccacgcggtt catgggctca tccaggtaac gcggctcgcc
     9961 gccgctctcg ccctgatagg acgtatcgaa ggcgagcgtg acaaagcctt ccgcggccag
    10021 gcgctttgca tactcaccag cggtctgttc cttcacgccg cctgcgggat gcaccacgac
    10081 gaccgccggg tagcgtgcgc catccttgaa atctcgtggc tggcacaagc tgccggccat
    10141 gatgatgttg cgattcttga agctgacgcg ttgcataacc ttctcctgtg ttgaaagatg
    10201 aacctggcgg aagctcaata gatcttgttg agcgggaaat actccacttt ctcaaagaaa
    10261 ggaaacagcg gaagttgcgc aatgagcgcc tggactttct ccacgctcgt ctcattgaaa
    10321 acaaccaccc cgccgccgtc attctctttg gcgaaaagat gttcgatgat gccctgatcg
    10381 cgccaggaat tggtcatggc atattcagcg gccatgacgc tcgcgattcg atcgtctttc
    10441 ttcgggtagg tgatgaccag tactctgttc atggttgcag actcctgttg attgaatgct
    10501 gaccttttta ttgtggcttc ctgacagcac gacgaacgcg ccggcttcca agaatgcctc
    10561 ggctgatgcc aaccccattc cggagccggc acccgtgata agagcaactg tgcctgatcg
    10621 atcgatgttc atttagcggt tccgtttgtt ttgcgtcgcg tctgctgaca cgaaccgggt
    10681 gcatccggtc aactgtatca gagaagctgt ccatatcgat ttgcgtcgga tcggggccgc
    10741 cgcgcgcacc ggtatcgagc gcgtcgatct tcgtcatctc cgagtcgctc aacacgaagt
    10801 cgaaaatgtc gatgtttttg gcgatcctgg cagctttgac cgacttaggg atggcgacga
    10861 cgccattctg gaactgccag cgcaaaatga cctgcgccgc cgttttccca tgcgcccggc
    10921 cgataccggt gatgacggga tccgcaagcg gatcgccggt gccggtggcg gacgctcccg
    10981 gccagtagcg tttcaccccg ccgatcggcg accacgcctg catcacgatc ccgagcgccg
    11041 cgcttgtctg tcgaagcgcc ttctgggcaa agtacggatg gagttcgact tggttgaccg
    11101 ctggaacgac atcggtgcgc gcgatcagcg cttcgagctg ctgctccgtg tgattgcaca
    11161 caccgatcgc ccgtacccgt cccgcggcca gcagcttctc ggcagcctta taggactgca
    11221 cggttgcgtc aaaattcttc gttggccagt gcaacaggta aaggtcgaga tagtccagcc
    11281 tgagtttcgt caggcttccg tcgaaaccgc gcagcgccgc atcgtagccg tagtccgtca
    11341 gccacaactt ggtcgtgacg aagagttccg cccggttgat gccgctgttg cggatgcctt
    11401 cgccgacttg gctctcgttc ttgtaggcgc gggctgtgtc gatcaatcga tagccatccg
    11461 cgatggcggt cgcgacggcc ttgatcgtgt cctcaggcga ggattgaaag acgcccaagc
    11521 caagcgccgg catctcgacg ccgttgttga gtttcagaag cggtgttttc atggcaatct
    11581 cctttcttat cgtttccagg ttgctttctc aatttatgct tttgcatggt ttatcccacg
    11641 cgagacgccg gtgactcgga tttgagcgac gccatgtcga tcgagaaccg gtacttcaca
    11701 tcggacttca gcagtctgtc gtaggcttcg ttgaccttct ggatcgggat gacttgtact
    11761 cttcctcttt cccgtccgtc gggttcgaaa gcacgatcag aacatagcgg gccatttgtc
    11821 gtttcataat aaagataaag tgagcctacg cggatttttg aacattcatc acgacaacct
    11881 gttggttctc acgtcaaagg ccccaagcaa gccgaacatc ttgtcgtgac ggatggcctt
    11941 cccgagaagc gcggcaactt cctgccgggt cacgacgcgt cctggagcaa ccatgaagga
    12001 gctgacggcg tgagagtcgt ccaggtccca ataaggattt acgtagacag gcatggtcgg
    12061 ctcgctgcgc cagctttcgg acagcggccc catgtccacg gcgtcgtagc caatcttatc
    12121 gagaaaatcg attacgactg ccttagcttc agcatggtca ctggagaccg gcagcgcact
    12181 gcgctacgcc gagccctttg gccgagcgcg gctcagcaaa cgaacaaagt ccatattgtt
    12241 caatgcacga acgacccgcg agcgggacag gtgcttttgc acgagctcgc ttgtggcgat
    12301 tgtgtcggtc ttgacctccg gcatcacgcc atcacgttcc ggatagtagt tcattgtatc
    12361 gatgacggtt tttcccgcca acgcttcggc ggaaagcttg gcgtacccgg cgaagggaac
    12421 ggcgagcacg atgagatcgg ctgcctccgt cacctccgca aaggtagcgg cccgggcgct
    12481 tggcccttgc ttagagacaa gctcggcaag tgtctctggt ccgcgggagt tgcagatcac
    12541 gacgttgaag cctgcggcga ttgcgaggcg ggctacctgg ccgccgatca ttccgctgcc
    12601 gatgattcca atggtttgtg tcatattgct cctacagccc cgtcatcgcc aactctcctg
    12661 ccgacagccg ttcgcctctc aaggtaagcc cggcaagcgt cgtttccatc tcggcgatgt
    12721 cgtccgcgct gagaacaaca tccaccgcac caatattttc ttccaggcga tgcagtttcg
    12781 tggtgcctgg aatggggacg atccacggct tcttcgccag tagccaagcc agggccacct
    12841 gagcgggcgt tgcgcccttg cgtgcggccg catccttcac caaagcgacg agagccatat
    12901 tggcctcgcg gttttcctgc gcaaagcgcg gttccgtgtt gcgatagtcc gactcgccga
    12961 acctggtatc agcttgaatc ttgccggtga gaaagcccgc gcccagcgga ctgaagggca
    13021 tgaaaccgat tcccagttcc tggcagagcg ggagaatctt ttcctcaggt ccgcgatgcc
    13081 agagcgaata ctcgctttca agcgcggtga ccggctgaac cgcgtgcgcc cggcggatgc
    13141 tttcgggacc ggcttcggaa aggccgaaat gcttcacttt ccctgcggcg atgaggtccc
    13201 tgactgcgcc cgccacgtct tccatcggca cctcgggatc gacccggtgc tgatagagaa
    13261 gatcaatgcg gtccgtcgcc agacgtttga gggaggcgtc gacgacggcg cgaatatggg
    13321 cgggtttgct gttgacaccg ccaagatgcc ggccggtttc cagatcgata tcgaagccga
    13381 acttcgtggc gatcacgacc ttgtcgcgaa agggcgccac cgccttgccg acgatggttt
    13441 cgttgacgaa aggaccatac gcctccgctg tgtcgaagag agtaatccct ttctcatagg
    13501 ccgcacgcag gaaggcgacg gcttcgcctt ccggcaacgc cgtgttgtag aacgaagtca
    13561 gccccatgca gccgagaccg agggcggaga cttccaagcc gctgtttccg agttttcgtg
    13621 tttgcatgat gtgatccttt cttaaggttg ccgtcattgc accgttttgt aaccatcccg
    13681 tggttagaac ctatctcaaa atcgttcatc ggtcggggaa ccgtttgctc gaaggggttt
    13741 gagccacgag caaattgcaa actccgatgg cctaggctca ggactcatta aaatccccat
    13801 caaccatctt gacgcggtcc gaatcatcga tcttgattcg ctacagcgca tgcgtcgctc
    13861 cgacaagtct ggccgtggct gttacgcggc caggcaggcg ggctcgcccc gcgcggtctc
    13921 cctatgtact gtgtcaagta cgtctcgacc tcgtggggct ccacgccctg tctcgcatgg
    13981 cgtctccgcc gtttcgtcac gaaccttcat gaataatgct tcctatgccg gggcgtcgat
    14041 ggattccacg cgcaccagct ttttgttcac gaactcctgg atgcccatgc tggaaagctc
    14101 gcgcccataa ccggaatcca tgatgccgcc aaagggcagg tccggggccg tccaggtggg
    14161 gtgattgatg aagaccatgc cggtgttaat gcggctggcc acgcgtttac ctcgagccac
    14221 gtcgttggta aaaatcgatc ctcccaaacc gaagtccgag tcattggcga gcaccactgc
    14281 ttcgtcttcg tttttgaccc ggaacatcaa tgcgacaggg ccgaagaatt cctcccggta
    14341 agcgggattg tcgggcttga tgttggtcag gatcgtcggc tccataaacg cgccgggccg
    14401 atccatccgt ttgccgccca ggacgaggtc ggccccatgc gcgacggctt gatcgacctg
    14461 cttgagcaag ttaaccagtg ccccttccgt agagagtgga ccgagcgtcg tcgccttgtc
    14521 catcgggttt ccgggtgtca aggccgagag cgcggacttg aatttttcca aaaaacggtc
    14581 ggcaagtttc tcgacgacaa tgtaacgttt tgcggcgatg cagcattggc cggtgttgtt
    14641 cattttggcc cagaccgccc acttcacggt tttgtccaga tctgcgtctt ccagcacgat
    14701 gaaggcgtcg ctcccgccca actccatggt tgtttttttg aggtgttttc cggctctctc
    14761 ggccacactc ttgccggcgt ccacactgcc ggtcaacgcc actcccttga cgcggcgatc
    14821 ctcgatcacc cgattcacct gttcatagga aataaacagg ttggtgtaag cgcccgcggg
    14881 cgcgtctgct tcaagccata acttttcaaa ggcgatggcg cactgcggca cggtgcccga
    14941 gtgcttcacc atcacgacgt tgccggccat cagattcggc gcggcaaagc gcgcgagttg
    15001 atagtaggga aaattccagg gctggacacc aaagagcacg ccgaacggag cgctttcgat
    15061 tttggcttcg cccgaaccgg gctgaagagg ctcgggggcg agaaaacgct cggcggtctt
    15121 ggcgtagtag tcgatgatgt ccgcgcttaa ctgcacttcg gcacgggatt cggcgatgag
    15181 ctttcccatc tcgagcgtca cagggcgggc gaattcgtcg atgcgcgccc gcatgatggc
    15241 ggcggctttt gccgcgatgg ctccacgctc ggcgaaggtg gtgcgccgcc agttctcgaa
    15301 acagtttgcg gcggtttcaa gcgccgtttc gagctgcgca tctgtcagtt gctcaaacgt
    15361 cttgacggtt gttccgtcga agggatttac actttggtag ctcatgatgt gatcctttct
    15421 ttaagattaa tgtggtcact ccatcacggc ttcgtgccgg ggtgagccgg cggtccaagc
    15481 gttcatgagc tggtgcacgg ctcatgctgt tgtggcgcga gcgttcaccg gttggtcatc
    15541 tgttcgacct tttcgggata acgcgcgccc tggacggtca gcttcgagac gccttgctcg
    15601 atctcgcgca ggtcgtcggt ggtcaaatcg accgacgcgg cgccgaggtt ttcttccagt
    15661 cggtgcagtt tggtcgtgcc ggggatcggc acaatccacg acttttgcgc cagcagccac
    15721 gcgagcgcga tttgcgcggg ggtgacctgc ttccgattcg cgatggttgt gagcaattcc
    15781 acgaacgcca gatttgcgcg acgattctcg gcttcgaacc ggggcagcgt gttgcggaag
    15841 tcgttggcgt ccagcttcgt gtccacgtcc atcttgcctg tcaggaaccc tttccccagc
    15901 gggctgaacg gcacgaagcc gatgcccagt tcttccaacg tggggatgat ctccgtctcg
    15961 ggttcgcggg tgaacatcga gtattcgctc tgcagcgcgg tcagcggctg cacggcatgg
    16021 gcgcggcgga tcatcgcgac gccggcttcc gacatgccga agtgcttgac tttgccttct
    16081 gtgatcaggt ctttgaccac accggcgacg tcttccatcg ggacgttcgg atcgacccgg
    16141 tgctggtaga acaggtcgat gcggtccgtc ttgagccgct tcagggactg ctcggccacg
    16201 aggcggatgc gtgcaggcgt gctgtccagc ccggcctgcg aattgccatc cttgaagccg
    16261 aacttggtgg cgatgatgat cttgtcgcga aagggcgcga cggcctcgcc caccatctct
    16321 tcgttgaccg cgccgtaggc ctcggccgag tcgaagaacg tgacgccgcg ttcgaacgcg
    16381 gcgcggatga gcttgacggc atcccccttg tcggtcgcag gaccgtagcc gaagctcagg
    16441 cccatgcagc cgaggccacg acgaggtcgt cccggtcgag cttgaattcc ttgatgagtt
    16501 tgccggtcac ctcttcgctg gtgcctagct ggtacacgtt ggccgtgtcg aagtagttga
    16561 tgcccagttc gatcgccttc ttgaagatgg gcttggcatc ctcgaagtcc acggcccagg
    16621 ggaacaggcc gtgttcactg cccggtttgc cgaaactcat catcccgagg caaatcttcg
    16681 agacgtcaat gcctgtgttt ccaagtttga tgtacttcat ggttttctct ttcctctatg
    16741 gaggtcgccg aaccaataga aaggtgatag gcatagcgca acctcaatgt gtggggggcg
    16801 tcattgccga tgaaagcgat agcgcagcca gacaatgccg tctggcaaag tctcgacggc
    16861 caccagctcc atcgactgcc cctcggccgg cttctcgccc ggcgcccctt ggcattcaaa
    16921 gatcgacgga atccccgcca acccgtcgat gccgggataa agcatcaggc tcagttcgtc
    16981 gatcaccccg gccttgagga aggcgccgtt gatggttccg ccgccttcga ccagcacccg
    17041 ggtcatgccg aagtccgccg atagcgtgcc caggagcctg ggaacgtcgt cgcccttggg
    17101 gccggcaaac aaataggaca cgccttgcgc ttgcagatgg tccagatact gctgcgagac
    17161 gctttcgccc aggacagcga tcaggttttc accggacacg ttgtcactgt cgtagcggat
    17221 cgttccctga gggtcgagca cgatcaggga tcgcttcgat tgccgatgac ccacgaaggt
    17281 ctggaaatgc gcgggtcgcg tagcgacatc gccctcgtac cgcaggggca gcgccgattc
    17341 gcgggccgtc cgtccaccga tgatctgcgc atccgcatca aacttcgcgc tgtattcgta
    17401 gtacggggcc gatgcctcgg tggaggtctt tccgctggtc agcggcgtgt agcggcccac
    17461 gtgcaagcgg ccatccacgg aactcagcat gtggcaaatg acttggggct tcatcttgtg
    17521 tatctcgctt tcttgacggt gaatgggcag cctcaaagca ccaggctggc gaggtgttgg
    17581 cgaaagcttt cgttgccgag cttctggcgc agcgcataga tgtcgatggc gtcatcgccg
    17641 gcgacgtagc gcaggtgatc ctggtcatcg gtggccgctt cccagactac ttgcgcgatg
    17701 gattcggcgc tggaaatctt cgccatgtcg ggcacgaaga acttcaccgt ggcctcgttc
    17761 atggcgtcat agcctggcgc gggcacgtgg tcgagcgagc ggctcatgaa gtcggtcatg
    17821 acggcgccgg gcgcgacggt cttgaccttg atgccgaacg gggccagctc gtaagacagc
    17881 ccttcgctcc agccttccag cgcccatttg ctggcgttgt agaccgagtt gagcggcagc
    17941 ccgatcaagc ccgccagcga tgtggtggtg atgaagcgcc cgctgcgctt ttgcttgaag
    18001 tacgggatga actcgcgcgt gacgcgcatc gcaccgaaga cgttggtctc gaactgccgc
    18061 acgatgcggg catcgtcaat accgtccatc gggcctgcca gaccgtagcc cgcgttgttg
    18121 aacaccacgt ccacgtcgct ggacttcagc gcgctttgga tcgtttcgct cacctgttcc
    18181 gagcgcgtca cgtccagcgg cagcacggtg acgttggcga actgggtcag ctcggtttcc
    18241 ttggcgggct gacgcattgt tgccaacaca ttccagccct tgaccgcgaa cagtttggcc
    18301 gtggctttgc ccagccccga agatgcgccg gtaatgaaaa tggtcttgct catggttgat
    18361 agctcctttc aagtcgtcac aaggcagatc aaggtgagac gtcttcccac cagcgaggga
    18421 atgtctgagc ggcgctcccg atctcggcaa cttctccgat gcgggggatc agcagcggcg
    18481 gggtggaaag gtgtcaggaa ccatttctgg tcttgccgca tgttgagcca ataaaaggtt
    18541 cctgacactt tattttccac aggcctctgg aatctcgcga ccgtagttct ccaaggtgat
    18601 gctgtccggc tccgggccgc tgcgcacgcc tgtatcgagc gcgtcgattg aagcgacctg
    18661 ctccttggtg agttcgaagt cgaacacgtc aaggttctcg gcgatgcgcg cgggcttggt
    18721 cgatttgggg atcactgaac ggccctcctg caggtgccaa cgcagcatca cttgcgcggc
    18781 tgacttgcca tgctcacgcg caatctgctg cagggtgggg tcgtcgaacg tcctcttctc
    18841 gccgcccccg taggacgtga tgccgccgag gggcgaccag gcctgggtca ggatgccgtg
    18901 ctcggcgtgg agacgttgca gttccttttg ctggaagtag ggatgaacct cgatctggtt
    18961 caccgcaggc accaccgatg tttctgccag aaggcgttgc agcacgtccg gcatgaagtt
    19021 gctcacgccg atcgcacgca ccttgccgtc ggccagcagt ttctccagtg ccttgtaagc
    19081 gccgagggtg agatcgaaac ggctggtgag cggttgatgc aagagcagca ggtcgagttg
    19141 atcgaccccc aacttgccgg cgctcttgtc gaacgcatgc acggtggcgt cgtagccgta
    19201 gtcgctgatc cacaccttcg tctgaatgaa gatgtcgtcg cgggcgagtc ccgaacggcg
    19261 gatgccttcg cccacttcgc gctcgttggc gtaggcggcg gccgtgtcga tgaggcgata
    19321 gcccacccgc aaggcttctt ggacggcggc agccgtgaca tcgggcgggg cctgaaacac
    19381 ccctagccct agcatcggca tgtgcaggcc gttgctcaaa gtggtcgttt gcatggatat
    19441 agcctcctca aaggttgctg acaccttatt gtcctttttg catttgtttt tcctttcgag
    19501 tatcgggtcg gtcactcagt cttgagagac gccatgtcga tggagaaccg gtacttcaca
    19561 tcggacttca gcagtctgtc ataggcttcg ttgacattct ggatcgggat gacttcgaca
    19621 tcggcggtaa tgttatgttc gccgcagaag tcgagcatct cctgggtctc ggggatacca
    19681 ccgatgttcg acccggacac actgcggcgc ccaaagatca acgcgaacga cgacagcgcg
    19741 aggggttgag acggcactcc gacgagggtc aggttcccgt cgaggcggag gagatgaaga
    19801 tattggttga tgtcgtgatc gacgggaacc gtgtcgagaa tgaagtcaaa gctgccggtg
    19861 tgctttttca tctcgttcgc atcacgggat acgacgacct cgtgcgcgcc caggcgcagc
    19921 gcatcttctg ccttactggg cgaggtggtg aaaaccacca cgtgggcccc gaacacccga
    19981 gcgaacttca cccccatatg gcctaagcca ccggggccga cgatgcccac tttcttaccc
    20041 ttggtgacgc cgtggtggcg caggggtgag taggtggtga tcccagcgca gagcagagca
    20101 gaggtgcggt gccggcgccg ttgaggttgg tgggggcgcg gaggacgaag cgctcgtcga
    20161 cgacgatgct gtcggagtag ccgccgtagg tgaccggggc ggtcttgtgc ttgtccggtg
    20221 agccgaacgt gaaggtctgg ttcgggcaga agttctcaag cccggctgta cagttcgggc
    20281 aagtcccgtc cgagtcaacc aggcagccga ccgcggcaag gtcgtcgggc ttgtacttcg
    20341 tcaccgctga gccgaccttg gtgacacggc cgacgatctc atgacctggg acaatcgggt
    20401 aggtagtgga catgacgctg ctccaatcgt tacgtaccga atggaggtcg gagtgacaga
    20461 tgccgcagta cagtatctca atctgtacgt catgttcggt tggatctcga cgagcgattg
    20521 tcgtagagac aagcgacgat gtcgcactgg cagcggagta agccttcgtc ttgtacatgg
    20581 atatatcctc ctctaggcaa aagatgtcgg tatacggtct gataaaccag acccgatggc
    20641 tgtttccatt tattcgacct ttaatccggc ctgatattgc tcgtcgcgga ccttttacat
    20701 tagtcgacgg ccttgaagtc gaatggatta ttcatttatc cgacgaagta ggatctttac
    20761 ccggatcgat cactagcacg gcgcgccaat tttcttgcag ggcacgccaa aagagttggg
    20821 agcttccctt cacgcaaacg tgatgagcgg cttcaacatg ccgtctgcct tcgttcgcat
    20881 cagctcaaag gccttctcca catcgctgaa tctgaatcga tgcgtcgtca gtgggagcgg
    20941 attgacacgc ccggtttcta aaagccgcat cagccgtttc atccgttccg ctccaccggg
    21001 gcagagtccc gtcctgataa ccttatcgct catcccaacg ccccattctt tgcgcggcat
    21061 ctgtacgtat tgtccctctc cgtgatagcc cacgttggaa atggtcccgc ccggacgcgt
    21121 cgctttgata caggcttcga acgtcgcctg agccccgagg gcttcgatgg acgaatccac
    21181 gccttgtccg cccgtcaagc cgagaatggc ttcgacggga tcctgttccg tgaagtcgat
    21241 caccacatcc gccccgaact ctttcgccag tttctttcgc tccgggacgg cctccactgc
    21301 gatgaccaag ccggccccca gtaaccggga tcccaccgtg gccatgaggc cgaccggacc
    21361 ctgggcgaaa accgccacag agccgccgat gggaatgttc gcatgctccg cccccatgaa
    21421 cccggtggac atcatgtccg cgcagtaggc ggcctgctcg tcggtgagac ccttagaaat
    21481 cggcgccaag ttcgcctgtg cactgttcac atgaaaatac tcggcgagac tgccgtcttt
    21541 cacgttcgcg aacttccacc caccgagcat gtcggtacat tgcgacgtgt aaccgcgcaa
    21601 acagttctca cagaggtaac agggcgtgat cgcgttgacg gcgacccgat cgccctcacg
    21661 aaatcccttc acggcgctgc ccaatttgta aatgaccccc acggcctcat ggcccagggt
    21721 caggttggtg cgttcaccga tcgccccgcc gaccgtatgg acatcggacg tacagatgag
    21781 cgcggccgtg gttttgacga tcgcatcatt cggtccgaga tcaggaatcg gcttctccat
    21841 catccccacc gagccgagct tcttcatgac aaatgctttc atggtctggg ccatggtttg
    21901 ctcctcctta tatctggatt gagttggggc tttctccatg cgatctgact tctcagacgc
    21961 atcgacctgt cagcttcggc ttccgaaagc cccggtctca tgcctgcatg cctcctgagg
    22021 ctacatgctt atgccatcag cacgtgttcg ctacttctca gaaattcttt ggcgatcccc
    22081 aaggcctgat cggttttctg aatcgaccgc tctgcctgtg actccagtcc cgtcagggcc
    22141 cgaatggcat cgatggtctc cggaacgacg atggcttggt taaacacttg gtaggtgtag
    22201 taggcctcgt tcccgttcac cgtgagcaca tcttcccaca gcgccacttc ccacagatct
    22261 ccgcgtcgac gtcccaagtc gctcatcagc tcgatcgtgc tattcagcgc cgccaccccc
    22321 tcgtgcatgc gaatgaaggc gatccgtggt gccaggcgga atgcggccaa caattcctct
    22381 ttgctcgccg aacgggtcag ctccacccac caggtgtgaa gatggctgct gttatgggac
    22441 gctttggccg cgatcgtcac gacatccaaa tccggatcga cggtcttcgc atccggaccc
    22501 tggtggctcg gtatggtctt ttccggaatg acggtgttca tgatgccgtc cagatgcgct
    22561 tcccatggat ccgatgctcg acggatcagc gtgcctcgcg cgcgtttgag gagtccggcc
    22621 ttcttcaggg caccgagcgt tcgcacaatc gaggtcgtgt tgcatgaaac gaccctggtg
    22681 ctggaacgac cgatggccgt ttcataattg gcctgggcca cgaaggaatg cccagtcagc
    22741 gcatgtgatt cgcccccctg gtagattgcc ctcacacctg cctgctcata gagtatcttg
    22801 ttggcagctc cgattttctt gggtgtgcaa tccgccacca cgtcgacctt ccggagcaag
    22861 tcatccaagg tgccagcgac tgggatgtcg gctgcgcgca tcgcctgggc cttgtccggc
    22921 agcgcagcat agatatccag cccaaggacc gctgccgtct tgatccggta gtcagtcacg
    22981 acatcggcca cgccgactaa ttccatatcc tcctgttgct tcaccgcatc ggccacgcgc
    23041 ttgccgatca ccccatagcc attgactgcg actttgacct ttctcatggg ttccctcctt
    23101 ctccattact gggattgctc gctgagcacg gcgatcccag gtagttcttc accgccgatg
    23161 aatttcaacg atgcccctcc gcctgttgac acatggctaa tctccgtctc cagtacttcc
    23221 gcttttcgca ccgccgccac ggtgtcgccg cctcccacaa ccgtgaaggc gccacgccaa
    23281 tgggccagtg ccatggcccg cgcgatgtcg actgtcccgt cggcacattc atcgacttcg
    23341 aacgcgccga ggggaccgtt ccagaagatg gtcccggttc cttgcagcaa ctcgtgcgcg
    23401 aaggtcagcg acgtatcgcg gccgatatcc aatcccataa agcctgcagg gatctctcga
    23461 tggacgagtt gcagtctgtc ccgctgctcc gcgttggcgg cgatgacgtg gtcctccggc
    23521 agcagaatgt gctttcgata ctgtgccagc acgtctttgg cccagggaat gaattcaggc
    23581 tcgaccggcg aatcacccgt cgccacgcct tgggcagcca ggaacgtata ggcgattccg
    23641 ccgcccagaa ggatggtatc agccttcggc agcagcgcct tcaacgccga cagcttgtcc
    23701 ttgatcttga tcccaccgac caccaccctg aagggacgaa cggggcgatc gcgcacctga
    23761 ctcaggacat ccatttcctt acgcacgaga aaaccggcca ggcggtctgt gaagagttgt
    23821 gctgctccgt acgtcgaagc atgggcccgg tgcatcgtgc caaaggcatc gttcacatag
    23881 agctccgcca gtgcggcgag ttgcttggca aagaccggat cattcgcctc ctcgcctggt
    23941 tcgaaccgca gattctcgag cagcagaacc tctccaggct tcaacgtggt gagcgtttgt
    24001 ttcaccgact cgccgaccgc cgcctcgacg aactttactg ttggaactct cagaatctgt
    24061 ccaaggcgat cagcaaccgg tttcagcgac agtgctggca ccactttccc cttgggcttt
    24121 cccagatggg aggcgaggat cactttcgcg ccacgctggg agaggtattc gattgtcggg
    24181 atggcttgcc gaaggcggta gtcctcccga atccgaccgt gatcgagagg cacgttgaag
    24241 tccacacgaa ccaagaccgt tcgttctcga tagcgatcat ggggtagatc agagatgatt
    24301 cgtttcatcg gtccgtcctc cctgtgtgtc tcagccagtt cgttgtgcgg gtcccctctg
    24361 taaaccctgt tccagtttca gaatggccat cgtggtcttg aggaccgcgt cagggttgag
    24421 cgagatgctg tcgatgcctt gttcgaccag gaacgaggcc acctctggat agtcgctcgg
    24481 ggcctgtccg cagatgccga tcttcttccc cttcttcctg gccgttgtaa tcaccgatgc
    24541 catcattgat gtcaccgcct ggttacgctc atcgaacaga tgggcgatga tctcggaatc
    24601 tcgatccacc ccaagcgtca attgggtaag atcgttggac ccgatggaga atccgtcgaa
    24661 gacctcagcg aactgatccg ccaacacgac attgctcgga acttcgcaca tgacgtagat
    24721 ctcgagcccg ttctcaccgc gcgtaagccc atgcttcgcc atttcagcct ggaccagtcg
    24781 cccttcttcg acggtgcggc agaacgggat cataagcttc acgttggtga gtcccatctc
    24841 gccacggact ttcttcatcg cacggcactc caaggcaaac ccggcctgat agcgtgaatc
    24901 gtagtagcga gaggcgccgc ggaagccaat catgggattc tcctcagtcg gctcatagcc
    24961 tcttcccccg accaggctgg catactcgtt ggatttgaag tcactgagtc ggacgatgac
    25021 gtcctttgga tagaaggctg ccgcaatcat cgcaacccct tgggccagct tgtccacgaa
    25081 gaactgggcc ttgtcggcgt agcccactgt cagtcggtcg atctcagcct tgaccgcagc
    25141 atcgtccaac cggtcatagt tgaggagtgc gaggggatgt acctttatat aggtggagat
    25201 gatgaactcc tctctcgcca gcccgacgcc gtcattgggg atgaaggaca acgcgaaggc
    25261 ctcttccggg tttccgacgt tcatcatgat cttggtcctc ggtcttgtga ggtcgctgag
    25321 ctcaacgcgc tgcacggtga aggggagtgc ctcttcatag acaaatcccg tatcaccctc
    25381 ggcacaggac agcgtgacca tctggcagtc ccgcagcacc tccgtggcat tgcccgttcc
    25441 gacgatagcc ggaacgccga gttctcgact cacaatggcg gcatggcatg ttctgccgcc
    25501 gcgattcgtg atgatgcctg acgccttctt catcaccggc tcccaatccg gatccgtctt
    25561 gtccgtgacc aagacatcgc cgctcgtgaa atctcgcagc tgttggaggt gtttgacgac
    25621 gcgaaccggc ccaaggctga ttttgctgcc gacacttcgg ccggtgagca gcaccttgcc
    25681 tttccgacct aaccgataca gttcaagcga gtcttggcgc ttttgcgact gcacggtttc
    25741 cggccgggcc tggacgataa agagttcgcc cgtatgcccg tctttcgccc attccatatc
    25801 catggggctg gtccgaccct tcttggcact gtaatggtct tcgatcatgc aggcccatcg
    25861 tgacaacgtg agaatctcgt cgtctgtgat cgcaaatctg gcgcgatcat ctggtggaac
    25921 cggcacattc ttgaccaact tgctcccgcc gacgtcgtac acgagcttga actctttgct
    25981 gcccagcatc ttctggagga tcggccggaa tccctgcctg agggtgggct tgaagacata
    26041 gtattcatcg ggattcacgg ccccttgcac gacgttttct cctaatccat aggaggcatt
    26101 gatcagcacg gcatcgcgaa agccggtctc cgtatcgatc gaaaacatga tccctgatgc
    26161 gctcagatcc gaccgcacca tgcgctggac accgatcgac aacgcaatct tgaagtgatc
    26221 gaatccctta tcgacccgat aggagatcgc gcggtcggta aacagtgaag caaagcaccg
    26281 cttgcaggca tccaggagcg cctgatggcc ctgcacgttg aggtacgttt cctgctggcc
    26341 ggcaaagctg gcatccggaa ggtcttctgc ggttgcgctg ctccggaccg caacatccac
    26401 cagatcaaca tgcccatcac tcagccggtc ataggcctct acgatggctc gttcgagatc
    26461 ctgctggaga ctggccgcca gcatggcctg acggatctgt ctcccccgct gacggagatt
    26521 gtccaggtcg cgcgtgtcca gcccacggat tatctgtgtg atccgctgat ccaacccggt
    26581 cgtgcgaaga aacgaacgat aggcctcggc cgtaatggca aacccattgg gaaccttcac
    26641 gccccgtgag gccaattccc ggtacatctc gcccagcgag gcattcttgc ccccaaccaa
    26701 cgggatatcg tcgattccaa tgtcctcgaa ccagcggatc gcggcttggg tcgtggtggc
    26761 ggtatacgac atgtctcaac tccttctgcc actcttctcg tggcgctact tttcaatggc
    26821 gaagcccgca gccttccagc cgtccaatcc accgtagagg ctcaggacgt tcgcgtaacc
    26881 gagcatcctc agtacggcta acgcgatagc accccgatgt ccggttttgc agtagacaat
    26941 caatgacgcc gctttgtcgc ccgggaggtc gtcgatggct gtcatgagac tgcggagcgg
    27001 gacgtttttg gcgcccggaa tatgctcaac cgcaaactcg tttggttcgc gtacatcgag
    27061 cacgtggggt acgccggtgg cgatcgcctt ctcggccgcg acgcccttga tgctgtagta
    27121 gtcgtcgggc atcgcggcaa gcatctgttc tgcggcggtt cgcaccggtg ttgacatcca
    27181 ctcatgtgtc gcggcggcat cactgacctt cgccgcatgt accgggccga ccagaacagc
    27241 gcacagaacc aatgcggtgc cgctgcatgc tcgttgtgct aaggccatgg ctgccatcct
    27301 ctctcatcga ttgtgcgagt tctgtcggca gaagagctgc caccccagca tcacatacac
    27361 gggtcggtcc cctctatttc acttctctca ccatgtcgat ggcgccgcag ggacattcct
    27421 ccgcgcagag cccgcatcct ttacagtagt cgtagttgat cgcataccgc ttcccaggtc
    27481 cgagcttgat cacggcgctg tcgggacaca ccgcatagca gttgtcacag ttgaagcagt
    27541 tgccgcagga cagacagcgg cgtgcctcca agagcgcggt gtccgcatca aggccgccga
    27601 cgatctcatc aaaactcgac cgacgccgca ccagattcag taacggctgc ttcgaggcgt
    27661 ctgcgtcggt gtagtaccag gtgttcaaac ggctgaaggt cgccggctca tgagcttccg
    27721 gtttgacgta cgcggagccg cgcaaatagg catcgatgtg gcgggcggcc ttcttcccct
    27781 ggccggtggc caccgtgacg gattgctcgc ctggcaccat gtctccgccg gcgaagatac
    27841 cgggacggcc agtcatcatc gtcccatcca cttcgatggt tccgtcctcc cgtacacgca
    27901 acccggatac ccgacgaaga aaaccggtat cggcccgttg ccccaacgcc agaatcacgc
    27961 tatcggcttc gatcctttcg aagcgaccgg tcggttgtgg tttcccttgt tcatcgagtg
    28021 ccatttcctc gactgtcaat gtcgattcgt caccttgcgc gatccttcgt agccattgga
    28081 actgcacgcc ttcgtcctgg gcttccgtga tttcgctggc aaacgccggc gcatggccca
    28141 aatctcgacg gtaaataatg atgaccggtt ccatccccaa ccgctcgaca ctgcgtgcca
    28201 cgtcgatggc cgtgttgcct ccaccgtaga tcgcgacgcg gcgtcctagc ttggggcgag
    28261 ccgccccttc catgtcgcgc aagaacgtga cggcatcgag aacccgcttg gcatcgaccc
    28321 ccggaatttc gacgcgccgg gctagatgcg cgccaagcgc gagaaaggcg gcgtcgaatc
    28381 gtccttccgc catggtcgcc tcaagatcgt cgactcggct gttcagccta atcgtgacac
    28441 ccatggattg aattcttgcg acttcggcat ccacgatctc acgcggcagt cggtacttgg
    28501 ggatgccgta ccgcatcatg ccgccagctt gtggaccggc ttcatgaatg gtgacctgat
    28561 gccccatccg agccaagtga taggctgtgg agagtccgct tggaccggcg ccgatgacca
    28621 acacgttctt ccccgtccgt ggagcgcacg ccacccgcca cccttgcgcg atcgcctgat
    28681 cacccaggaa ccgttccacc gcgtgaatgc tgacggcctc atcgatatgc ccacgattac
    28741 aggcgctttc acaggggtga taacagacgc ggccgtggat cgcgggcatc gggttgtcct
    28801 gcatgagctg ttgccatgct tcttcgtagg ccccttcttc agcatgatag agccaggcct
    28861 gaatgttctc gccggccggg caggcgtggt tgcacggtgg caagcgatcg acatagatcg
    28921 gtttcatcgt gcgccagttt cccgtgtggt tggccaggct cgtgttcgga tcgagcgtga
    28981 tggcaaatgg tgtgcgttcc ataggtgcct ctattccatc agtccataaa cagcgatgtt
    29041 gtcatctgcc attgcttgga tcgcggcgag ggcttccgtg tcctgcggcg gaccgaacag
    29101 atgcttgaat cgagtctgta gctccagata gcgttcgaca ggaactttgc ggcgaatcgg
    29161 cgtggagcag atgacttttc cgaattcggc ctcgaacaga gggaacagcc cggattccac
    29221 cgccaggcgc gccaccttca tcgtgtgtgc ggggtccgag ccccacccca gcggacaagg
    29281 cacgtgcaca tggatatacc gagccccgcg aacaccgatc gccttcgtga cttttcgttc
    29341 caggtcatgg agatcggcca cagaggccgt cgcgacatag gggatcccat gcgcgagggc
    29401 aatccgtggc acgttcttcc cgttcccaat cgggttgccg ggatgctcac ccacggcttg
    29461 cgtcgtgttg gttcgggcga acggaggggt cgcccccgag cgttggatgc cggtgttcat
    29521 gtaggcttcg ttgtcgtaac agagatacag gacgtcatcg tttcgctcaa acatcccgga
    29581 cagacaaccg aacccaatgt cgaccgtcgc tccgtcaccc ccttgcgcga tcaccgtcac
    29641 gtcgtcacgc ctcttggcac gaagcgccgc ggcgatcccg ctcgccaccg ccggtgcatt
    29701 gccgaacagc gaatgcatcc agggaatttg ccaagcgctt tcgggatagg ggctggaaaa
    29761 gacctccaga cagccggttg cattcacggc aatcaattga ttgtggctgg cacgcatggc
    29821 ggcatcgagc gcgtaacgcg cccccagcgc ttcgccgcaa ccttggcagg ctcggtgacc
    29881 ggagttgagc gaattggtcc gatccaaccg ggattggacg gtccgcgtcg cttcatcgag
    29941 caaacggttc ccagccgtga aggtgccggt ctgataaaac tttatgcgtt ccgtcgtcat
    30001 cgcctcgtcc atcaactcac tttcgttccg atcacattca tggtttgcag catatgctcc
    30061 gcgactggac cagcccgtcg gatgtcgcgc cgtcgagcga gctcctgctc gatcactgtc
    30121 ttgttgaggt ccaagaagta cggttctgtc agacgttctg cacgagcttc caggatcatg
    30181 tgcttcaacg aagatgtgga gatggaacgt cctcccaatc ctgcaatgac ggtgtggacg
    30241 gaaatctgcc gtccttggag cgctgattgg acgtcggcag agacgatccc tcccatgccg
    30301 acggcaaggt ctttctccac tacaatcacc cgcgtcgttt tttctagcag tgcgcttacc
    30361 tgaaacttcg gaaagggccg atagcatccg attttgacgc agccgacccg gacacctgtg
    30421 tttcgcagtt cgtccacggc gtctttgatc gttccgatga ccgaccccat ggcaatcacc
    30481 gcgatgtcgg catcctccat gcgataagcg gagcagagtc cgccggatcg tcgctgaaaa
    30541 tgttgctcga agacctccac caattccggc atgacctcta gcgctcgaag atgttgttgg
    30601 tgcgagaaat atttaacttc ggtaaacgcc tccggcccca ccatggcgcc gatggaaacc
    30661 ggacgttcag gatcaaggac ctgtaccgga tcatagggag gaagataggc attcacctga
    30721 acggcgtccg gcacgtccac tggttcgtag gcgtgcgtca gcacataccc gtccatgcag
    30781 accatcaccg ggcaactcag cgattcggcc agacggaacg cttgaatgtg gagatcgacg
    30841 gcgtcctgat tatcctcggc aaacaactga atccacccag catcgcgcaa cgccatgctg
    30901 tccgaatgat cgttccaaat attgatcggc gcgccgatcg cccggttggc gatggtcatg
    30961 acaatcggga gtcctagtcc ggctacgttg tagaccgcct cggccataaa gagcagcccc
    31021 tgactgctcg tcgccgtgta ggtcctcgcg ccggaagccg aggcgccaat caggacactc
    31081 aaggcggcaa actcggattc tacgttcaga aactggcagc gtccgatgtg gccctgcttg
    31141 gtcttgcgtg ataaccgttc gacaatgtgc gtctgtgggg aaatgggata ggccgcaatg
    31201 acctcaggtt ggcagagtgc aatagcctca gccacggctt gggaaccctc aatctgcatg
    31261 cgcatggtgt acgattcccc tttgttcagt aatagaagtg gtgacaacgt caaacgcttc
    31321 ccgcgcggca gcccgattcg cctccccgac cgcacctggg aatttctgct gaatcgctga
    31381 ctccacggag gcaagccgga tcagtccggt gatcgcggcg aacgcaccca gcagtggccc
    31441 attggcaatg ggtcgcttga ggtatttcag cccgaggtcc gtggcagaga acgcacacac
    31501 atggccgagg ggcagcgttg agagaaattc ctcaatgtgg agtgcatcga gggtctgggt
    31561 cgaattgatc aacacaaacc cgaggggggc gagccctccg aacaagtcga cctgatgcag
    31621 gagcgtcgga tcctgaataa tgacaacatc agggcgcgtc accgtttcgc gcgtgcggat
    31681 ggctccctca gcgacgcggc agaacgccac aaccggtgct cccatccgtt ccgacccgaa
    31741 gctcggaaag gcctgggcat atttgccgtc catgaacgcc gccatcgcga gcagttctgc
    31801 cgccgtcacg accccttgcc caccccgccc atgaatgcgg atttgccaca tcgtgtttct
    31861 catcaccatc caccgaccat gctgatcata cgctcggctc caacgtgctc tggtacgcgc
    31921 agtgtgtaga tacctggtcg gctcaactgc ggtactacac cgccgtttca actgagagga
    31981 tcaacggcca ggaagaccta attccacacg aggcctcggc attcaagaac gatctcatca
    32041 cgctcgcagc tccgaaaacg aatgacgacg cccgcgatcc gacggttcct atccgtctgg
    32101 ctctccatgg acacctctct tgggtacggt gttgggagac gtcgaatctc cgacaagatc
    32161 cactccgtct cctgcgccag ccgttgagca ctgcaatttc acgaagttac tgtcacatca
    32221 actccgcgac tcaatgttgg acattgggga gattggaaac aatttcgaga tgacgctgtg
    32281 aagaaactcc ccagaaatac acctttcttg atctgactgg atgacctcgt gactaaggcg
    32341 caaggtccag caattgccgc aagggacaca cgtgaccgct gatggaaccg atattggtct
    32401 tgataatgct cagggtgatg ttcttggctg cactgccttg tggagaccct ggcttgtctc
    32461 gacagacaat gaattgctcg gcgcgtcaac gccacggcaa atcctatttg gtgactgcgc
    32521 gatccagaat gactcgttct gcatccagcc aatcttcgag gtggtagcca tttcggcagc
    32581 cgcgcacaac atacagttca taggctcgtg ccgtgatgcg ggcgtgtaaa tcgtcgactg
    32641 cagcttgtgc cgaacctgct gctttgcgag ttgacacagg cacgttaggc tgctcaacct
    32701 gcatcgtgtg cggtttcttc tcccggaacg gttgtgtcgt ctgagccttc atgagcgtct
    32761 cctttaacgc taaaaagttt ttccctccgt acgagtctcc ttgctggaaa gaacgccaac
    32821 agcccgtcat tctgtgcaac tccgcacgcg catgtgatgg agtcacccaa taggcccgca
    32881 gaaacgttcc gcgcatcagc actgaaaaca ccaccgcgaa cactgggcca gaagacatcc
    32941 acgttcaggc ccccgtcctc tcacctcaca ctctggcgta ctgccgcgcg tcgtggaaga
    33001 cattgctcgc gagatggacc gtgtagcacc ctcgctcttt aggcatcagc cggatcgccc
    33061 cctctcgact gagccgatca atgacgatga agacttgatt ccatgtgagc tccgggcatt
    33121 cgagcacgat atcatcgaga gtgcgcccct gtgaccgcac aatcacttcc agcactcgat
    33181 catccgtctc atggtgactc atcatttccc ctcctgcgca atcacatgac gttcctatgg
    33241 atcccgcaca ctgcagcatg ggtgccagtt acaaattctc ggggcgggtt tcttcagatc
    33301 ctcagaacaa cgggagctta cgcgggagga agagaggacg gtgcggacgg atcgcgctgc
    33361 atcatgggga gtttcggtac attcattccc gcctggttgt cgcagacttc ctcaacgatg
    33421 tggctaaata cttttaagag tcttgttggt ggttttggat cactggcatg tcgctcgctg
    33481 acgaacaacg ttcccgagat acaacatgtc ggtgagttag ggagatgctg acgacctgag
    33541 cgccggcgac tctggcatcc tgagccacgg tgccggacag gatcactttg gctcccgcca
    33601 cgatcacatc gccgttgacg atgccggaaa tttccacatg cgggccgaat gcaaaatagt
    33661 ctccctgtac gacttgcccc gtacgcaaga cggcccgctc cttccacggc gcgcgagaca
    33721 acgatgcatc ggcctgggcc gtggcaggca gcatgcacag gatcaacaac atcacgatac
    33781 gtgtcgtcat gtgagttccc tccgaaagcg ctgcaggcca cacccgacct ggaaccgcgc
    33841 tcatcgtggc agaacgccgg caccgaatcg atagaccaga tccccgccga aataactcgc
    33901 aatcaacaac aacagcccgc cgctcaggcc gatgaccagc accagcatcg gctgttcacg
    33961 catccaaccc caacaagagg ccagacgcag cccgagcagg ccgagaaata cccagaacac
    34021 ggagccgccc agttcctcat gaatttcgat ggcctgtttc gggatgccgc tgcgttccac
    34081 cgcctcctcg gcgaaatgtc cggtgagcac cgccgctccg gccgccagga tgccgagcac
    34141 cagcagagac aagctggtgt cccgaaactg gtgcctccgc caccgaaacg ccaacgcatc
    34201 gaacagggtg ctcgccaaca acaatgcaat aggaaaatgg acgaccatgg gatggatcgg
    34261 atgcacgctc gctccttcgc tcaatagggt gaatcgacca ggtctcatcc ggaatcgcca
    34321 aggcacagat gccctctcaa gatcgacgcc ttccagatcg gaagtgcaag gatggagccc
    34381 tccttcttcc cggcgcagag gcagctgaaa gcttacagca agatcagaga tggagatgcg
    34441 agaggaggag aaggggatcg gaatcgggga ggactgctac agagcgaatc cttggctctg
    34501 tggcagaatg caacagggcc aatggatcaa aacaagacac agcggctacc ttggcatcga
    34561 acggcccatc gctcgctcct ctcaaagcac ccgagctgag gtctgttcct gtacagaaag
    34621 caggaagaga tgtttgcgac cgggttgccg aaactcctgc agcttcccta cgaccaaccc
    34681 tgcttgcgac tgcagcgcag cggcatgttg ttcttcagtc accacccgga tggccccatg
    34741 tgtgcgcacc gcttcacgca ccgcatcctg actcggcaga tccggcacca cattgtcgcg
    34801 cttcagataa tacagggcat cttcattctt gggccacccc gggatataca tgaacagggc
    34861 gggctgagtt cccagggcca gggttttgat ttcctcgacg gccttccggg gcgacgcggc
    34921 ctgatcgatc gcgggaaaca cgtagtttac caccagcacc acgtaagcga ccgcaagagc
    34981 cccgaccatg ccgagtgcga ggcgtacgcg tgatcgaagc acggcataga ggagcaagcc
    35041 ggccagtgaa acgatcgtca cgatgaacat cggcgacacc acggcagtag acaccagcca
    35101 ccggcggcga agcggtgctg cgccgaagaa catcccggcg gcgaacgtca cggccaacac
    35161 acccagcaac acacggagca ggggcgtcat cgcagcagag tcctcggccg acgagaacag
    35221 ccggtgatag tagtacccga tcatcaagcc gatgccgggc acgatcgtcg tgaggtaggg
    35281 ctcccgtttg agggtcgcca gactgaaggc cgtaaagagg ctcaacaccc agaccaatag
    35341 gagcaactcc gtgggatgag cacgaaacgg gcgctgggcc cagagcaaca ggagcgccga
    35401 cggaagcacc gcgcaccagg ggaaaaagtc ggcccacatc atccccagat accaatacag
    35461 gggatgcccg tcggtagccg ggccggacgt gcgggtgaac gcagtattcc agaccgactg
    35521 gatcgcatta ctgattctga aatggtgctg gtacccggga cccagcatct gcgcgtatcc
    35581 cgccatcagc gcgacagcca gcgcgaaccc ggcccagaag tatccatctt tcagcatacg
    35641 cgtatcgcgc tgaatggcgg catacaacgc catcacgagc aacgggagaa aaaacccgtg
    35701 catctctttc aacatcgcac cgagggccat gctcacgaag gccagcagat accagcgtga
    35761 gcttcgcccc agcagttgca cctgaatcca cgcgaagagg gcgagggtaa ccagaaatgt
    35821 gagggtcgaa tcgaacagca cacggcgccc gtaccagagg aacacatggc tggtggcgac
    35881 caccagcgcc gcccagaatc cggccgccgg agacaccagc ctgactccaa gcacatagat
    35941 caggatgatg gtgcccacgg cggccagcga gccgggcagg cgcaaggcaa tttcgtggtc
    36001 gccccatatc gatgtcgaga gattgagcag ccaaaaaaac agcggcggct tattgaagta
    36061 cggtacatcg tgataggtca ggtcgaaaaa ttcacgccgg cgccccattt ctcccgcaat
    36121 gccggcatac agtccttcgc tcccctccaa taacgacaga tggagattga caaaataggc
    36181 ccaaccgccg accaacgcaa gcagcgtcat ctcaagacat cgacgcgcca ccggattcca
    36241 ccactccatc ccatcttccc cttcccgttt cattcctcct cctatgaaca ctcattcgac
    36301 gcttcgcagc atattcaaca tatcggcaga tttcgcatcg acatccacga agtgtgtgat
    36361 ggagccccca aacacacgca ctatcaacgg ccaattaatg gttcaaacat ggcgggcaac
    36421 gagatcgatc atgttggaca gcttgcaaat ggggttttgg cgttccgacg caactatgct
    36481 catggtcccc gaccaaccct tttaccatca gccccttaca cccgcatgcc cctgcgctct
    36541 atcatacggc ttcacgcaac aagaccacaa ttgatcgagc cctcacttgg cagccaagcg
    36601 accgacgaac cggttctggt cccgtttcga aaagctttcc caggctcggg aagctcgtcg
    36661 atcacccgcc tccagtccgg cacctgactt tcctagcagg atgcggaaaa agtccgccag
    36721 cggcattctc gcaagacact gccgcctcac tatctcgacg gcgttcacaa gcgtggtgcg
    36781 ctttattcag cgcgtcgtga acctcaaacg ctcaacgtac ggccacggga aaagggcttg
    36841 tcttggcagg ctcggggtgg gcgggtacga atgaacgatt cgcctccggg acaccgggcg
    36901 ctcaccgact cgcgcccgtc cacagacgtg acgctgcggc cttgctggac ggcctttttg
    36961 cgcatcctgc ggacgattct gacaccgaca cggcacgtga gctaatcgcg gcgtctgacg
    37021 caacaatcga gtttttccgc agcctgctgg gagcctgtcc gacattagga aacacgaaag
    37081 tttcgtgtgc acacattcgg ctcaggatca acgatttcaa ttcgatcaat atgatttcat
    37141 cctcccatat cagtcaattt cggcaatgtc ggacaggctc ctagaagccc aggtagggga
    37201 aacaggcgtg cctccgagat gggcgtgcgc ggtgaaccga cggttccaca cctccacccg
    37261 ccaatttctg agccgacgaa acgggagagc cgtcctcatg aaagcaggcc gctgggtagc
    37321 gcacacgcaa ccacgcctcg atcatcccga ccagtggacg cgggaggtgg cggcggaatg
    37381 ggtggccgcg gtgctcagca tgaagaaggg ggacttctgc cagaccacct acctgcggca
    37441 aggacagcaa ggcatcccca tttcaccgcg acataaaatg caactcctga gcgccttgaa
    37501 cgtcttcttc cgcgactgcc aggagtggga atggatcccc cgtcgatttg atccccgacg
    37561 ggcgtttgcc gttccgcgca tgctgctggc tctcattcac ccgaatccaa aacctctccc
    37621 cgacgatctg tgggcgaagg cgctgtcagc gggattacag ctgggagccg atgatctagc
    37681 ctccctctcg ccacggtggc ccacacgcag attctacccg attgagatgg tccgcgcggt
    37741 cgcgatggtc tggttatttt ccgggctgcg cagcgatgag atcgtacgcc tccgagtcgg
    37801 gtgcgccacc acgaaagtgt tggacgagca gcgggggaaa accgtgtgtc tgctcgaggt
    37861 gccgacgaat aagacctccc ggtccttcgt gaaaccagtc gatcccatcg tggctgccgt
    37921 catcgacgcc tgggaacagg ttcgcccaca gcaggcgcgg ttcatcgacc gcaagacggg
    37981 agaaccagtg ccgctcttgt ttgcctttcg aggaagacca ttggccccac cctacctaaa
    38041 tcacacggtc attccactca tctgcacgaa ggccgggatc cctcgccatg atcgacatgg
    38101 gacgatcacg agtcaccgcg cccgctccac catggccacg caactcctga atagtcctga
    38161 ccacccgatg acggtgtttg aacttcagca atggctcggc caccggagtg tgagttcgac
    38221 cttgcactat ctggctatca cgccgacacg cttgtctgag tcattcctga aatcgaatca
    38281 tacacatcgg aatctacgaa cggtacaagt gctgatcgac caagacgtgg tcttgagcgg
    38341 ggacgcgggc acccagccat ggaaattcta cgatctcggt catggatatt gccggtacga
    38401 cttttttgaa cattgtcccc accggatggc ctgtgccaaa tgttcgttct atcttcccaa
    38461 aggttcaacg gaggcgcagc tgcttgaggg gaaaatcaat ttactgaaaa tgcgccaaga
    38521 gatcccgctg caaccggcgg aacttgcggc cgtggatgaa gggatcgagg cgttcgaacg
    38581 gctcctcact cagctaaggc agacacccac tcccgctggt ctgacccctc ttcaaatcga
    38641 tgacgcgaaa acctcggcga gggaaagtgg cgtgcaggct gggctacccg aaacatctgc
    38701 tgcacgaccg acggtcacct aatcactgtc atcagctcag caccagcgta atcccaaaga
    38761 tggccctatc gggtgaggcc aggtgccccc ttcttcctga gcagcactcg ttcggtgggg
    38821 tttgcatgac atggcatgcg agccaactgc tcggttgaac actaattcca cataattccc
    38881 acttatgcga aacatgctcg ctaaagctcg cgcgctgggc cggtgaaccc caactttcgc
    38941 aatgccgacg ccttcaccaa actcgtgctg cagccctatc gagacgcgca gttcggtcca
    39001 aacaccggcc cttttctcta cgagttccaa cggcacgggc tgccggcgga agaattcatc
    39061 gggaaactcg atgacttctt cggcagcgtc tcaacggact tccgctacgc cgtggagata
    39121 cggaatgctg gcctgctcgg gccccgctac cacgacatgc tgagggcgca cggagtcgct
    39181 catgtctaca atcattggtc ttatatgccc ccgctggccg aacagcatca acgcatgaag
    39241 accatgacgg ccccgttcac cgtccttcgt ctcttgaccc cattaaaaat gacatacgag
    39301 gcagcgaaga aacgagcgga accctatgac aaaatcgtgg gggagctacc agagatgcgc
    39361 aaagatacag tgagtcttat acaggaggct acagcagaga atcggtcgac ctatgtcctc
    39421 gtcaacaacc gagccgaagg gaatgcccca ctgacgattc aggcacttat cgaccgccta
    39481 caaaccggag gctgaaacgc aatctggacc cacaactcct atgcagctgc actgtttgca
    39541 gcgactctcg acgcgaatgg ggaaaacgtc tgtgacgaga tcgccttgag ggcggccgcc
    39601 ctggccttga gggcccgtcg atacgcccca tcttctccat acttcaggat ggagaacttc
    39661 ttgtgccggg attgacagtt ctcgatcggc cattggacgt cccagtagag ccgatggacg
    39721 cggcgcccct tggagaattc ccatcggtcg atacagctca gccctgaaat gccagagcga
    39781 ttcgtcttct tgagaatggc acagtagact ggcttgctca gcggaggatt ggacctgatc
    39841 agtgcgtccc ggtaggtcgt ggctgcaacc aacgcctgct cagctccgcc gtagcggccg
    39901 tcactgaagc ttcgcacgta aatgcgggta cgacgttgta cagtcacccg ccaacagtgc
    39961 gtgtgggagg cctcgtgatc aatgcgatga atattcaaca caagccagcg tccaccttat
    40021 gcccggcgag gtaggactcc aagtaagcgg catcttcacc tggaatcgtt ttcaaacgta
    40081 ctccgaattc ttccgtccgt acccacgtca ccaaagcctc atccaacacg agagcccgac
    40141 gctttgtcgg caggacaacg aataaggaca gcgtcatccc cggcgtcact tcggcctgac
    40201 tctcgacatg gcagtggtgc attgagagcc caacaatctt gcccacgtcg tagagattgt
    40261 cttgtgctga atggaacaca caggacagag gcgcgtcctc gaaaacgcta tttggcagtt
    40321 gggcaatgga catagatcaa agtgtactca cgctatcgac tttgtcatat tcctctttcg
    40381 acgggaccaa gaaccactcg cccgtgtatc acgatctaaa agccctggtc acctgagatc
    40441 atatcttcac gactaggagt ttttgtctca gtctttcatt gatatcccgt cttccccggt
    40501 cggtttctga cactccaact gatcccattc tcctgcgcga acggcctcat gacgcatccg
    40561 catcagtctt gccacaacct cgagtccatt caacacatca accgggtctt tggcgtctag
    40621 gcggagagtc agatcacgaa cccaccaatg agttgcgggg gaagacaata acatcgaccg
    40681 cataacgttg tattggtcag cccatcgcat gggatcgcct tatgacatgt tctattgaaa
    40741 ccaccttttg agtattacct gcgtgagttg atgtaagaat aatcgggcgc aaaagggaat
    40801 gctctatatc accaatcgca acctcatgga ttcaggcatc tcttgatcaa cacttcaatg
    40861 ctgcaaagac cctggccata gacacgtcga ggagtgaagg acattccata caccaacaca
    40921 accagctaaa aagccgacac aaaccccacg atgcacaatc ggacttccat cctatctctt
    40981 tgaaaagcgc accccgggag tctacttcgc ccttccaaag atcacctact ccgatacggc
    41041 cattgccgtt ccgttagggg gaaccccaat cacataaggc aaatgctcct acgctcagat
    41101 ggggaaacca cttaaaatct ccttgggcac tgtcgcctac agtcacttga aaccgatcat
    41161 gcgcgaagca cagcaatgcg tcgcactgac acccgagagt cttgaagagg agattgcacc
    41221 atgcccgccg gcattaggac atcgctaaca cgggtctata cgtatcccgc atatctcaca
    41281 ttttcttcct atgctgcctt gccggctgcg gtcagagaag aagctgatca aatgcaatcg
    41341 ctgtggaatg cgatggtcga cgtttatgaa gtcgcccatc gccaacagga gcgccttcac
    41401 aaacaattcc ttcaaacgag tcgtacaacg acgcgcgcag ctggcggcac acgttcaatt
    41461 ggcgatccct ttgagtgcat caaacaattg cgtgaccaaa acgactccct cgaggacgag
    41521 actcttcttg ccctttctcg aacctatcgc acagctgcgg ctgagtacga agcctccgaa
    41581 caccacaata tcgaccgatg tctcccttac cctgagagca tgaagcagga gctttcccag
    41641 atcaccaaac gtctgcatga cacactgcat caactcgcac gcacaagccc tctgtctcgt
    41701 tcacacagca atgccgtgct cgagcgaatg aaaaccgcca tccgccgcgt gcccgtcgga
    41761 aatctcccac ccagcaagga aacaggtcca cctacctcgt tattctttac ccacgacttc
    41821 ggttcgcaag gaattcgaat ccaatcgttg actcgcgcag acgtgaccca gtcgggctcc
    41881 tcatttgccg ccaatcgcct gcttacactc cacccggtgt cgcaattcgc agaggacacc
    41941 gatccccgag aacgggggca acgagatttt tgcagaactg agggggagct tttcgttcgc
    42001 aacgtcccaa ttccgtttga aatcatcctt actccgcttc tgccaaaaga cgctcttctc
    42061 aaacaggttt ctctcattgg ctcacaacag cgaccacaga cgagcgtcaa catggacagt
    42121 tccaacgcat gtctcaggaa cgacacgaca tggcagtggt accttcaatt cacgctaatc
    42181 gtaccgccga cccagcaatc ggaacccaaa aaatacggga tgatcgccct tgattttgat
    42241 catcgactca taaatgacgc taacccgctt gttagggtag gagaatgggt atctgacacg
    42301 ggcaaacacg aatccgtctt ctttcccgca cgggtggtaa agaatcttat cgcggcatac
    42361 gaagccaggg aagcgcttaa tgttcagcac ctcgccctca aacaactatt tgttcatttt
    42421 ccatttgctg gggcactcgc cgatatggtc aaggagccca ttcacagcga cgtgccactg
    42481 agcagaaaga ggcttcgatg gattgctcat caggcagtta aacaggccta cagcggtccc
    42541 ctcacaagca taatccgaga aatactctcg caaatccgag acacacgaat cgatgtagtg
    42601 agacggcgcc ggcaatggcg ccgagaccga gccgagtttt acgacaaact tgcgcatcgc
    42661 ctttcgagag aagccgggac tattgtgctg actccagtat tgagcgcagg gcgttcaggt
    42721 gccgagggct cttctaccag tacgacaaga agcgcgccgt tagcacaagc cctcgcacat
    42781 cccatcaaca tgcaagagtt tattcaccgt ctacagcatg ccgcgcccat ccatcggacc
    42841 acactccatt ttgtacaatc catgcatgcc ctgatcgcct gacttacgca gctcttgcaa
    42901 atagactccg acatcctcaa ccaagagctt cgccccaaca cactccagtc cttgagccag
    42961 cgcctaccac gacaataacc accgcctcac gatcccccct gcattgtgca caccataatg
    43021 aggacctcca tgcttggcac tgtcgatttg ctcccacacg cccaagtggt tacgaacaac
    43081 atacctgcca attcttcctc tgctgacccg cctcagagtg gggacgtttc ctcagtcatg
    43141 ccccagctag caaccgagcc agttcctatg aagaccgagg cggtgcccgc tcctccgccc
    43201 ccacggctgg aagaagaaga cgacgaagac gaacaggatc caattgccag cttcgaacag
    43261 gactccaacg attttcagcg agctacgatc tccatcgtga tgacgctgct cccaacggac
    43321 caccaccctg acggccgcct cacactcatc ggcgtacgga gtcacaacct cccgccgcag
    43381 ctgactaccg cacgattcaa cgacttactg ccccttccgg aatctctgag ccaggccctc
    43441 gcaagttggg agcagacctt tactgcagcg ttaacggcac ggaaaaccca gcgggacgca
    43501 aaagccgcgg agcaacaggc caagacagtg aaacaggaga agaaacgcga agcggaacgg
    43561 caagccagaa aacccaaagt tgctaagccg gccgccgcga aggcgccgcc cgtggcccta
    43621 acacccaaac cggatcgtga acaacacaca cacgattcca cctcaaacac agccctccct
    43681 caagccggct tattcgagta gcgaaaggag ccttatggtt acgcttcaac ttgaaggaca
    43741 agaaattcaa ttgccccccg aaatcgccat cagcgacgat gcggttcgca aagcgattgc
    43801 ccccctcttc ccgcaagccg cccatgccac cattcagcga accactgaca cgaatgggat
    43861 gacaacgatt aggctcacga aacaggctgg aacgaaagga aattaccatg tcatcctccg
    43921 tcagcttatc gagtcccccc gccacgtcaa tcccgccctc acgctctacc aggcatacca
    43981 agccgagtcc cagccaaccc agacgacggt cgaatccata ctcctcacgc agcaaaagct
    44041 gcgtctcgcc attgagcacg gtgaaaagga atacaaagag actgctacca ttaaggagcg
    44101 actcaggaac actcctggct cgcccagccg cctccttcca cctgggttct aacccgccct
    44161 ccgcgccatt aagcctcgtt ctcgcatccc tcaaaaacat agaggttatc tcggtcccct
    44221 catgcctcga atacctggag gaacgagagc gagacttttc tatcgtcaac gcgttccacg
    44281 agatctttcc ccggaggttt cgcacgaccg ccgccaccct tccgcatgga agcatcaagg
    44341 aggccgcaga acaacttcaa gaagagttca ttaagctaat cgagcaacac tacttcccgc
    44401 tcgatagctg ggacaccgaa tggattcgag accgcttccc attcattccc atacatctgg
    44461 aaaattatga ttgtgaatcc gaattcgagg aggcaccgct tccaatctgt atcgcagcgg
    44521 cctgggtggg acactattcc tacgctccca cctggacgaa cattcaggaa gaactcagcg
    44581 agcacactac aattccacga tgctttttga atcctcaaca ccgatgcaac gtccaatttc
    44641 ttgacttcag aagacgctgt gcgcgacgca gctttcccat ttcaggattt ccccaggtca
    44701 tcgacatcct cggccactgc accggatctc tgttcctcga catcagctac gactacgagt
    44761 ccccagatca cccgtaccaa tggaataaac gggatctcaa agctttggct cgccaatgga
    44821 aacgggcgca aatcatgcaa caatccatgc atagcacgac taaagcctta ctcgacaatc
    44881 cgtcagcatg ggaaacgatt ttcaggtgct ggtcagatct ttgcactgca ccacatgagg
    44941 cgcaaacatg agaggtgcga gctcggatca gcacgacgcg tcactgcact tcatcggcgg
    45001 gcaattgctc cttgaataca aagatggtga agcggttatc cgaaaatgta tttcaccaga
    45061 ggctgccagg aatgcgttct cttcagccgg gatagattcc gactggctcc cagaacatgt
    45121 ctgccgctat ggcataggcc ccagaggccc gtggctacta ctccgctttg cccctggtcg
    45181 ataccaaatc ccattggctg accccattac actttccgga ggcggagaac cacacacaat
    45241 gcttgccgtc ccgatgccag ggttgctgtt tctcgggtac gggacccact attacgtctg
    45301 ggcctacaag ctatggaagt gcgcggcaac gaagctcttc aaagcaccgc tcgctaacgt
    45361 gtaccctgat ggggcgatct gtttcggaaa cgtccatcca cgggtcgccc acggaaacac
    45421 gattacaaac agctggcgac tattctggga tagtaacttt tccgaccatc tcgcgaatgg
    45481 gaaatccaac gcccacccgg acaatatcct acctttcctg gcgaaacttc actcaactga
    45541 ggcacaagac tatcctctcg atgatcttga gccggctcac ctctcaattg ccaccatcat
    45601 tcggcaactc atgcagagcg gaaaagggta atcacatatg acgacggcgt ctcccttcat
    45661 aaaccaccac attgcgagtg gtccgcttcc ggcctccaat tccacccttt acgactatat
    45721 tctggctggc aacggggtgt ttattcgcgg caaacgtaga gagctgtcag tgctttttcc
    45781 agttgtcgaa catccaattg ccggccttcc acccattgca agcagcctca cactcacgat
    45841 tccgagaatt ccacaacggc tcatccaaga gatgattgaa gaggcactgg gagcctgcgc
    45901 agagccaacg ggtcccctgg aaagtctatt ccattttgaa cacgacacag attggcacat
    45961 gactattcca gaccagattc gtgcgcccct ctcagtccaa cctaccaccc ctgaacgctg
    46021 tccatcttac gagcgtgcca ttgtcgaaat tcactcacac cacaccatgg ctccacggtt
    46081 ttccgccgtg gacaatgaag acgaaacagg gtttcgaata tacggtgtgt gtggatggtt
    46141 gaccactcgc ccgtccatta cgtttcgtgt cgggctgtat ggccatttca taccaatccc
    46201 agcggagttc gttgcagagc tccctcccac acttcaggac acgtacacac catgagggac
    46261 gagatgtcgc cgcgacgtgt tgtcgctaca gggaagcgca gcacagaaaa aagcaccagg
    46321 acacacctag atcttagtca tgtgcacgcc gtgaaagtgc ttcctaagca gtgcccacgc
    46381 ctccatgtca tccaaatcgg atgtgggggc atcggagcgt ttctcggtat ccacgtcgca
    46441 cgcctcgcac gcgagtgttt tcgtctgttt gatacggtga tggtcacttt ctatgatggg
    46501 gacacaattg aagagaagaa cctccgccgt caaaactttt gtcaggcaga aatcggacac
    46561 aataaagcag aggccttagc atttcgactc tccaccgctt gggggattcc cattcgcgcg
    46621 tgtccgcggc atttctcggg gaaagaggcc catcctgagt acgacaccct gaccattctt
    46681 ctcggttgcg ttgacacagc aagagccagg cgcagccttc atcacgcgct cgcgcgatgg
    46741 aacaccagaa acgatgccca ggcatggctg attgatggcg ggaacctcac cacccacggg
    46801 caagtgttag ttggcaacgt cgtcacagac tttaacccac acgagagctt tcagctacgc
    46861 actgtgtgcc agtcactccc cgcgccagcg cttgtgcacc cagagttgct caagcgagaa
    46921 cgccgccccg tcaatactca acagcggtca tgtgcggaac ttgccctagg tgacccgcaa
    46981 tcgctcacga tcaatagcat cattgcctcg cacatggcgg actacctgct tcgactcgtc
    47041 atcaccaaag accttcgccg tttcgcaaca tatatcgata tggacactgg ctcagcgcgt
    47101 tctctggcca tcacttcgac agaaatctcc cgtgtattag gcttacccca caccgagtcc
    47161 atgggtaggg cttcgtagcc atgaacgcac gacacctctg aagaaagtca gtaatacaag
    47221 gggcaatact tcgatcaaag gaggtcaagc tccgacgagc cgcatcgaat gcttaccttc
    47281 ttggaaatgt cgtacgcatg gagcgcctgg atccaacgct ggggcctttc cgctatatta
    47341 tggattttta cggcgagtct gacgcacgcc gcacagcctc tcccccaaga actgatgaat
    47401 caatggtttg cggttgaagt cttgagtgga gccagaattc cgctgtcgat tgaattcgcg
    47461 ccctctccat ggtcactgat ccggctacgg ctggcgccgg acgaacgact gttggtgtta
    47521 tttaatgctc ccgtacaaga ctgcacgatt agtggcgacg cccggctcga tccagaccga
    47581 gatcggatcc acatccgaag ccatgccatc acgtgccgta ctactagagg atttgaaact
    47641 agagtcttcg ctggaacggt ggtttctgaa actgacgggt ttcaaggggt cccgttgtca
    47701 ttggcacatc aaagccagga ccagaagggg tacatcgtac cagccggagt ccagggcacc
    47761 ctggtcgtcc tcccgcacaa ccctcccgag cgagtcgacg ctcctccccc atctcgcaag
    47821 taaacgctgt ccctctcttt cgctgcccgc atccgctgca cattcttggt gccttcagtc
    47881 tctcggtgga ggcattttaa agatgaaacg gagttctcct ccacactgaa tgcccctcgc
    47941 cgtgcgaacc atacggggag cgggatgcac tgaagatctc caccatgggc tgaaagtcgc
    48001 gcatgccagg actgtgtagg atgcatacgt cgcggccgtc gcgacccatc agaggggcac
    48061 gaaacactca ttcgcgtgtt tggcaagcgc gcaggtcacg tgcggtactc gcagatggag
    48121 cagcgccatc ccgagtgcag gggcgcgtac gaacactatc acgcggcaag tgtagcatga
    48181 cgtgaatgcg tggcctaagc gaggagaagg agctagcccg ccaataccag gcgacggtgc
    48241 ccgatcgctg aagagaaaga cgtcagtatg aaagaagcag acgcacccct aacgcaatgg
    48301 atgccaggac tatggcggat caaactcagc tacaatggtc cggtgagcat tgtcacgcca
    48361 cgagcggacg gggatgaaga tttaacgatt gcggtgatcg ctgagcacct caacgatgga
    48421 aacaaagtcc tagcaaacgc atacctgatc gctgcagctc cgaaactgta cgagcaacta
    48481 gcactcttag tccgatgggc agaggaccag ggcgagtcat gcagattcgc acaggatgct
    48541 ctcgctgcgg cacggggaga atcccacaca acgtgctcgg gcccatgcat tgcggggctt
    48601 atgccgggcg aggcgtgtga gcaatgcgga aagctctgtg aagatagtga gacagagcta
    48661 tgaaactgcc tttcacagcg agcccatggg tctcgtagag atctttgagg cggtagaagc
    48721 tatctcgact gtagcccatc acctgacagg cctttgaggc atcgcctagc tgcttcgcca
    48781 gttccaacac gcccaccttc gtcctgatga tcttttgttc cgtcatcatg gtcctcgctt
    48841 ctttcgtgct gaggactcta tctgtctgtc agagaggtgg ctccggttgg ttggacagtg
    48901 taaccccttc cttaagtggc gcctggcggg ttgctgatta cgcggcggtc cgccgtgtcg
    48961 tcagatggtc ataatacacc tggtcaggcg tctggccgtc aagcgcctgg tgcggcctgg
    49021 tctggttgta gaacgtcagg tagcgtgcta ccccctgctg ggcggcgctg atggtctcgt
    49081 acgcgtgcag atacacttcc tcgtatttaa tgcttttcca cagtcgttcc acgaacacgt
    49141 tgtcccgcca acagcctttc ccgtccatgc tgatctggat gccgtgatct ttcagtaatc
    49201 cggtgaactc ctggctggtg aattggcacc cttgatcggt attgaagatc tccggcgtgc
    49261 catagtgggc cagggcgtct tggactgctt ccaggcagaa gtcggtggtc agcgtgttgg
    49321 acagccgcca cgccaacacc cgacgactgg cccagtcgag caccgcgaac agatagacga
    49381 agccctgcgc catcggaatg taggtgatat ccgccgccca gacgtgattg gggcgactga
    49441 tcgtcagatc cctgaggcga taggggtaga tggggtgggc cggatgccgg tggctcgtgc
    49501 gcggcttgcg atacagggcc tccaggccca tgcggcgcat cagggtcgcc acatgtcgcc
    49561 gtccaatggc ctggccctct tgccgcaaga gatcgcgcag catgcgggcg ccagcaaacg
    49621 gatgacgcag gtggagttcg tccagccgac gcatcagcgc cagctccgtc cctgacaccg
    49681 gcgtcggctg gtagtaggcc gtcgagcgag acaacccgag gatctgacat tgccgaacga
    49741 ctggcagggc atgggttcgg ttgatcatcg ttttgcgctc aacaatcccg ccttgatgag
    49801 cgccccttct aaaaaatcat tctccagggc cagttggccg atcttcgcgt ggaggacctt
    49861 gagatctggc gtgtctgacg tcggtgtggt gccgccaaat acgtccgcgg cccgcgccag
    49921 caattgttgc ttccattcgg tgatctgcgt gggatggaca ctgaattgct cggccaactc
    49981 ggccagcgtc ttgtcgcctt tgatggcagc gacagccact tgcgccttga aggcggctcc
    50041 atgattccgt ctcgttctct tcattgcctc gctcctctcg taggccacct cgcggtggtg
    50101 tgggtgaagc caggctacca cttagcacac tgtccgaatt tccggagccc tctctcagat
    50161 taagtgttag cttttaggcc aaactagtat tgagcctcct ccgaatccca caaccacaca
    50221 tacacaagcc attcaaactc agagccggcc gcgccaccac ctcccgacaa gcgattactc
    50281 gcatcctccg cctagaggac tcttcccatc gatctggagt gccatgccaa caccttttgt
    50341 ccatttgcac acgcacacca cttatagcct gctcgatggc gccactcttc tcgatcccct
    50401 cattgaacac gtcagatggc tcaaccaacc tgccgtcgcg atcaccgatc atgggaatct
    50461 ctttggtact atctccttct acaccaaggc acgatcggca ggcgtcaagc cgatcattgg
    50521 gtgcgaagtc tacatggcca ggggcagtct gttagacaaa caacccccag agggaccccg
    50581 tgatttctat cacctcatcc tgctagccca gaacagtatc ggataccaca acctcattcg
    50641 cctggtcagc cacgggtaca cggagggctt ctattacaaa ccccgcatag acaagtcctt
    50701 gctgcgcagc cacagttccg gcttgattgc actgtctggt tgtctcaccg gtgagatccc
    50761 caccctcctc cagcaggaac agttcgcttc cgcgtccgtg gccgcccaag aatttctcga
    50821 catcttcggt cctgatcggt tctatctcga actccaagct aatggacttc atcagcaaca
    50881 gagggtcaat caaggcctga ttcacttgca ccgggagctg gggatcccgc tggtcgccac
    50941 gaatgactgt cattatctca aacgaggcga tgcagacgcc catgacttat tgctctgcat
    51001 ccagaccggc actacgatca ctgatacaac cagactccgt tttggaaccg accaactgta
    51061 cgtcaaatcc tccgaggaga tggccgaagc ctttcgagag ctacctcaag ccatcacaca
    51121 cacgaatctg attgctgaac agtgcacgct cgagattcct ctgcatcgga cacaacttcc
    51181 tcggtacgac gtgcctcccc cgcacaccct cgacagtttt cttgaaaccc tcgcccaaca
    51241 agggttgtcg gcacgcctcg cccaccactc gggtcctctc gaccgcccaa cgtatgaacg
    51301 ccgcctgcgg accgaactcc tcaccatctg ttccatgggc ttcgccggct atttcttgat
    51361 cgtgtgggac atcatgaagt ttgcccgctc ccggcgcatc ccagttggcc cagggcgcgg
    51421 gtccgccgca ggtagcctgg tggcctatgc gctcgggatt acagagttgg acccgcttgc
    51481 tcacgacctc ctcttcgagc ggtttctcaa tccggatcga gtttccctcc cagacatcga
    51541 tatggatttt tgcatgaaga gacggcagga ggtgatcaac tatgtcacgg agaaatacgg
    51601 ccgagaccat gtcgcacaaa tcatcacctt tggcacccta ggcgccaaag ctgcaatccg
    51661 cgatgtaggc cgtgttctcg aaattcccta cgatgaagtc gacacgattg ccaagctgat
    51721 tccgtctcag cccaacatga cgatcgcaca ggcgctcgcc gaagaaccac gattaagtgc
    51781 cttgtctgcc tcgcaccctt cggtcggcac cctgctatcg gtcgccgaat cccttgaagg
    51841 gctcgccaga catgcctcca cccatgcggc gggactggtc atctccaata cccccctgct
    51901 ggagacagtc cccttatgcc gcaccacgag cgacgacatt gtgacgcaat ttggaatggg
    51961 ggaactcgaa cagcttggcc tagtgaagtt cgattttctc ggattaaaga ccctcaccgt
    52021 gatcgatcac accatctcac tgatcaatca gcaatccctg ggatcccgtc cgttgacgct
    52081 ggacgacatt ccgctcgatg acgccaaaac cttcgccctc ctcgcaagcg gacgtaccac
    52141 aggggtattt cagctggaaa gcaacggaat gagggagctg cttcgggaat ttcagcctga
    52201 tcggttcgaa gatatcacag cgattattgc gctctatcgt ccggggcccc tcgatctgat
    52261 tccggagttt attcagcaga aacgcgggca atatcctctc gcctcaaaca tcgcggccct
    52321 cgaaccgatt ctcagcacca cctatggcgt gatcgtctat caagaacaag tcatggccat
    52381 cgccaacacc gtggctggat tctcactggg ccaagccgac attcttcgcc gggctatggg
    52441 caaaaagaaa ccagaagaga tgcgcgccct gcatgcacag tttgtggaag gggccgtcgg
    52501 actcggcacg gagcgtgagg cagctgaaca cctcttccag atgatcacca agttcgctgg
    52561 ctatgggttc aacaagtccc atgccgctgc atacgcgcta ctcacctatc ggacggccta
    52621 tcttaaggct cacgatccgc tggcctacat gactgcgttg atgaccagtg acatcggcga
    52681 ccatgagcga ctctctctct atgccagtga atgccgggaa atgggcgtca ggctcctccc
    52741 gcccgacatc aaccggagtg agaccggatt cacgatcgtg gacggggcca tgctctatgg
    52801 tctggccgca atcaagcacg taggagaata tctcgccaca cagttgatcc agattcgtgc
    52861 aaccggaggc accttcgctt ccttctttga tctttgcgac cgcgtcgacc gccgcactgt
    52921 caccaagcgc gtactcgatg ccttgatcaa atcgggcgcc ctcgacacgc tctgcgacca
    52981 tcgcgcacaa ctatccgcaa ttctagatga tgcgacgcca gcgaaaccca ctcgatctca
    53041 gcggaacgtc acacaccaaa ccctgctcca actctttccg gaggagtcag aaactcgcgt
    53101 gctgcccaaa atacctgcct ggtctccatc tattcgattg gaccacgagc gagaagctct
    53161 cgggtgctat gtcagccacc atcccgtacg tgtccatgac caccaactga agcgtctcca
    53221 agttgttccc ttaactcagc tccctgacct cccggatggc aaagaagtgc gaatcgcggg
    53281 attgatcacc cggatcaaac gcatcctgac caaacggcag gaaccgatgg cctttgtcca
    53341 cctagaagat caggatcgca cactggaagt cgttgtcttt ccaagtctct atcgacacga
    53401 ctcagggcga ctggaacctg gcaccgtcat cgcgctcgaa ggtattcttg atcagaatga
    53461 ccaggggata aaagtgaaag ccaccagatt tcttcccctt gatcctgctg cctaatctcc
    53521 ccacaaacca gggagagcac tgcccatccg gtaatacaca cgcaacgacg acatcacctc
    53581 ggcgtgggtt tcatcaagaa tgagttgcgt tcaaaaagga gttgctctca ccatgacggc
    53641 cacactgact tccagcttac ctctgtcaga gatcagtgca tttctatcgc ccgtacgacg
    53701 ctggagtcgc cacggcctca tgacactcgg gtgcagtctc ggcatgacgc tcctcggtgc
    53761 gggagtcgta ctagctcagc ccgtcgactt gcctgcggtc gcgcgatgcc aaatggaggc
    53821 cgactcgcag cccaaccggc agacactcat ccggaactgg attcaccggg cagggcggac
    53881 cgatgcgcta gcagcgctct gtctctcatt ttctccaaat cccaacgcgg gggaatccct
    53941 ggcgtggcta cggcgcgcgg cggatctcgg actcccggac gcacagctgc ttctcggtgc
    54001 agaccttgag gtaggattca agagtcctcc caatagccaa gaagccatgc gctggtaccg
    54061 gaaagccgca caggccgatt tcccctttgc ccaatacgag ctcggtgcca tttattcgga
    54121 aggcagggcc aaccaacggc aagacttgcc tgaagcctac tactgggcgc agcttgcggc
    54181 ccggcatcaa ttccgaccgg ccaaggaact cgcagatacg ctcagtccac agctctcaca
    54241 tgatgagcgc gcgcgcatcg atgcccgcat caagaaatgg aagcgcgtcg tctcgtagcc
    54301 tatcctgtgc cgaaccgatt cggatttact gtgttggtat gggaccccta gccactagcc
    54361 ttcgtcagat tatctcgacc atcattcgat acctgccggg aggcgcatcc gctcctcgtc
    54421 tcgcccacga ggaagcccgt gcgcaacaag aacgtgtcga tctcgccatg ttgcagatcc
    54481 tcgtccaaga gagtgaacgg cctcacaccg acgtgagttt gtcagcctct ccattgaaag
    54541 acgaccccca tccctaagcc cgcagggccg attgcccccg ttcacacgct tcgatagaat
    54601 ggtgtccatc gagttgatga ggcgctatgc cgaccatgtc gaccgttgtc ctcctctacc
    54661 ttctcgggta tctcccaggc gccccgcttc ctcctgggat cgccccaccg gtgcctcacc
    54721 ttcgatgtga gaccttcacc gatagcgagt atcttgcgag tgaggaggac atcctcgacc
    54781 aaattaaaga ccgtggtcga tgtatcgacc ccatagtctc gtatgcacca tcactggatg
    54841 acccaagttg gtggatgaac cccgctccga ctgagccggc cgccgcttcc ttttttgatt
    54901 tcactgcatc ccggtagatc acacgtctgt caggctgccc catcacgata ccaacccatc
    54961 tcatgcctca gacgacggcc gctccacaga tcctgccgac ctggtagtct ggttcactcc
    55021 cctctagtca ggctcaagtc gcacaggaag gctgtatgcc ctacgccgaa tccttctcca
    55081 tcgcgtcgaa ttccaagcca gggctttgct ataccgtcac tcgaagccag gccaatgact
    55141 ggcaatgcac ctgtccacat tggacctacc gacgagccga gtgcgcccac atccggaaag
    55201 cccagtctct ccccgcaaca gagtcgatac aaaaaccgga accgccaatt gtctatgccc
    55261 atgtccgaga agtgacacct caaccagacg gatcgttgta cgtccccctc ctgccaattg
    55321 gggacccaca ttttctctat acactctgct ttgatctcta tgcgcacggc gtactttggg
    55381 cgagcattct cgaacggtat cggctccctc ggagtttcag cagagacact tgcgtggctt
    55441 tcattgaaca gcacggaaga aagatctatc agttcagcga caatccccca cggccgcgcc
    55501 accaatacca gattctctcg ggcattccac ttcccgacat tccacggtac caagaaacgc
    55561 cgctggagta ccacgcgcgt accggatacc ccctcgatct ctgccgacgc tatctcaacc
    55621 cctaacaacc caagtagcaa cagggcattc ctgccactac tgcacatgag gacgtatgaa
    55681 ccaacctccc attccaccga tgaggattca agaatctgga tatctcctcc atccgcctta
    55741 cggaaaccgg tatgttgcga tccaggttaa tcccgccgaa tggacacacg tgtgctcagc
    55801 acgcttcgga ccaggaagac cctgcagttt tagcaagcag acgaagtgga ctggcgactc
    55861 ctaccgctac aatattttca tccgtccaga gcatcggcag attgcactgc gatggtttaa
    55921 cggaagcgga gaaggctggc tcatcgcgga ggataaaaac gagagagatg aacgctccct
    55981 cctcgcccac attgcccagg tcccagacga agcgagacgc tgggacttct gtcatttcat
    56041 ctggagtgcg ctgacacaaa ctcggagggc ggtcgttcat acgacagagg acacctggga
    56101 acaggcgatc ctgcaaaagc ggatcacaat ccggaaacgc aatggccgac ggactgcatc
    56161 gatcaagccg cagatcatcg gattaccaaa cacagtatcc accttcaccc tttagctgcc
    56221 atccacgcac aggactgact ccaggacacg catgattcgc gacgaaactc agttagcctc
    56281 ccctcaacga tttcgatccc cggacggacg gctgtatatg ccgtcctatc cataggcagt
    56341 agattgtttc tcacgcatgt cagacggcat tcaccatatt gggagcgaca ctacactcac
    56401 gaaatgcgaa gcgatgagac ggcacaacgg tgggtccttg gcgaccccac gcgatccgcc
    56461 acgattcgtc gccgacgcga taatcgggag gttgtgcggt attgggtcga catcgagggg
    56521 ttacagtata agaccaccgc tctgaaatag cggaatctga ttggcagcgc gtttcccatc
    56581 aaagccacac acatccgtct actcacgcaa cgcaggttct gcaacgacaa ttttgagagt
    56641 ctgtccaccg attatctcga tttcagtcct tcgcagtcag taatacatct acatgaacgt
    56701 tatccttgga ataggtcccc acccctatgc ctcgcacaac cactttctcg atcgacttcc
    56761 ggaacgagat gctggagtac cgcagtgaat ccatttcgct tcaggccatg ttctggagat
    56821 ggctctgtct ctttgcgttt cgacggtgga aaacagagtt ccgagggaac cctgtcggag
    56881 ttcccggtga gcgggatccc aggttcccct gtcaaagcta tatgccgagg ccgcgggcaa
    56941 ctcacgacat gccgcattgc aggtttccgg acgggcacta cctctgttgg acttgtacag
    57001 caaatgccca acttcccaat cccggcaccc tgattgctct caaaggggcg agccctacgg
    57061 agttgaagtc tcgcacccaa gccgttccct cgactcacga ggcgctatga aaaaggatta
    57121 cgccgtatcg gtcatcttcc ctgaagtgtt ggtcaagcac agccggcaac acatcagaat
    57181 ctcggcgagt aatctacggc tggctgcaaa tgaggccctt aaagccatca tgaaacggga
    57241 agggatcaaa ggacgacgac tgactcgctt gcaactggcc gtgatccacg ccggcacgag
    57301 cacgatacag gaccacgagg cctaagcgaa cacacctcat gaaggctatg ggtcttcgtc
    57361 ttcatcccca gtggggtagc gtcctacgct gacaaatact caccctcagt tgcagcgcga
    57421 cgatgctcat gctctgccgt cgtcaggaag ccatggccac agcggacaat gactcgtttg
    57481 gcgttggcat gacgatcatc gcagcttttt ttgccaacca gctttgtcgg ctcctcgagc
    57541 cgaaacggtt agggtacacc ttcaggagtc tgattctgct caacgccacg accatcatcc
    57601 tactggaaat gagcatcgac tggacggccg tgttctattc gctcgggccg attggactcg
    57661 ggctcctcga tatcttgaac aagacctcgc gcttcggtcc tttatatccg caactgtggg
    57721 ggtcccctgg gccatccaac tccacagagc catggcgtcc gaccgatctt gggcacggac
    57781 ggacactgat gtcagtgtgc cgaagacaga cgcctcctga tggatgagtg acgacggatg
    57841 tttactaacc ggatggcgct cacgcgcagg gcaaccgcct cgctgatacc acgacgccct
    57901 aaccgagcac tgacacagcc ctgagaacca tgacgatgtc tctcccatct actcattcaa
    57961 ccgaaacgcc acagccaatt ctctctattg ggatgctctc gcaacgcatt cgtcgacaac
    58021 tcgcgccgat gctgaccaag cagacgtact tgctccaagc ccaatggatc aaaccccagg
    58081 ccaataaggg gcaaaagggc ttcttctcgg tcactctggc ggacacagac aatccaaata
    58141 tggcgattga cgccatgatc tgggaaccag cggtcatcag gaaggtgctc gaacaaggcc
    58201 aacagttcgg ccacgacctg ctgagtcggg attctcgagt cgaggtcgtc gtcgaggcca
    58261 cgctggggtt ctgggccaat aagaacacga tctatgtgct cattcaccag ctcagcacga
    58321 tcggcatgct tggactgcga cagcaacaac gggaagcagc tcttcgcacc ctcaaagagg
    58381 agggcctcct tactcgcaat gccagccttc catggccacg attcccattg cgaatcggca
    58441 tcatcggcaa gcaaggaagt gacgccgttc acgatatctt aaatgtcctg cacagcagtc
    58501 cgtaccgatt tgaaaccact attttccaca ctgccgtcca aggagtcgga gcgatcccag
    58561 ggcttctcaa ggcattccac gatgtcgccg cacacaccac accgctcgat gtcattgtca
    58621 tgtcgcgtgg aggcggaaat gaaatagatt tggtcgcgtt tgataacctc gacgtcgccc
    58681 gcgccgtcgc gacagcaagc aaacccgtcc tgaccgggct gggccaccac ctcgatcggt
    58741 cgatctgtga cgaggtggcg gcaaaagctt tgtctacgcc aaccgctgcc gggcagtacc
    58801 tcgtcagcca tttggaccgc atccgcgaat gtctcttcac gacccatgca acgctacatg
    58861 cccatactcg acacacgttg ctggaatgga ctcatgcact cacgctcacc cggcatgccc
    58921 tctgggaacg cgcccggaac acgatcgagg ctcacgcgac agcgtcccac cacgcccatg
    58981 agcgattcca gcgcgcactc cgcgatcgcc tcgaagccag ctaccagcaa ggaaaaaccc
    59041 ttcgatacag ccttaccgcc agcctgacac aagcgctctc acagttgtcc atgggatacg
    59101 cctttacact ggtacgcctg cataccgccg gactttcgcg cgtcgatacc ctaaaaggcg
    59161 ccatcaccgc gttcgtccag gccttgaccc tctcagaccc acaacatctt cttgccagag
    59221 gctatgccta tgtccttgat aatcagggac ggatggtgac ctctcatagg acggtcatcg
    59281 cgggagcgca gctacatatt cacgttcagg acggcaggat taccgccacc acccttcaca
    59341 cggagccttc gcatgagcaa tgaagacacg tcccaccccc agctgcccac gtcctatgac
    59401 gcggcattca aggaattgga agacctctcc gccagagcta cgaaaggcga tatcaacatc
    59461 gatgagttga gcacggccat tcaacgaggg aaccagctgt ttgcctttct cagcagtggc
    59521 atcgcgaaga tcaaagctga gatcgaggac cacgctcagg acggtatcac ccaatccaag
    59581 cccaattaac cgcttggcat ccgatgttcc agggaccatg tccccattca gaagaggact
    59641 cactcagtca tgtcgaaaca ccatcacact cacccgatca cgatcgatca aactctggat
    59701 ctcttagcca agcagtggac ggctgcagac cgcatcagac tcagaaccgc ccggcaccgt
    59761 ccacgccatg tacgggccgc cgaactctgg acccgtattc aatcgttgac tcaatccacg
    59821 ctggccctgt tccaacaccc ttcagccatg gagacctcgc atggccatca ttctgatcgt
    59881 tgacgactat tcagacatcc gagcctgtct ccggacgatg ctcgaagagg cgggacatat
    59941 catcgtcgaa gcaggctccg gccaagaagc actgcatgcc tggaacgaaa cccgcccaga
    60001 tctcgtaatt actgatcact caatgcctgg gatgtcaggt gtcgagttga ttcgcgcttt
    60061 aggccagcgg caaccggaag tgaagggcat cttgatgtca gccgatcagg atccgcgccc
    60121 cgcggaacca ccagaccaat cccacgcgtg gccattcctc cagaagccct tcggcttccg
    60181 ggcgctacgg caagccatcg attcgcttct gctggctcag ccggcggaac cagcctagag
    60241 catccgccaa gcgtctttcg cgcctcttca ccccagacat ctgcgttcgt ccacccttcc
    60301 ggtctccact gcatcactaa cccgtcgctt aatgggatgt accaggctat ggccgactca
    60361 gactttctgt cgtatgcaca actctgtcag tttgtgcggg agcagggacg tctcccacgg
    60421 ttagatgacc cgatcccagc ctatcgttac gccggatggg ccctaccaat gatttgggaa
    60481 gggcatcgcg tgctgcctgg ggttccaaat cgatggggtt atcatttaga catacttcac
    60541 gcgagacgga ttccggacgc cccgattccg cagatcgaat ttctccacac ccctcatcga
    60601 gacacactca cgcatctgca acgctgggtc acgctcgccg ccgagcatca gtccagctgg
    60661 acaggcctca ccaatctcat tgcttggatt gcgttctctt taaaacttaa ccacgtcccc
    60721 ccgtcgctcg cggacaacac gcaagccgca ctctaccaag ccgtcaatct ccaagaattg
    60781 gtactccacc cttacgatta ttggggcgat atcatcgcgg aggggatcgg acgtgggccg
    60841 tgggccaatc caaacgggtt ctatccgacc cccatgagcg tctgtcagct catcgtacag
    60901 atgatgctcc ccgacgccga cagcctctcc ccagccgcac ggaacgcctt gcgggtcaaa
    60961 tccattgcag atcccgcagg atccggaacc ggaaggatgc tcctgctggc atcgaacgtc
    61021 agcctctcgc tccacgggat cgaaagggat gcactggtcc gcacggcatg tctcatcaac
    61081 ggcgctctct acgccccctg gctcgcattc cccttgcctc aagaaattct ccatggcacg
    61141 gacgcggaac atggcctcac aaaccaagcc tcgctcctct cagtcgccac gccaatacct
    61201 gaagagattt cgctcattcc tcccatcaac gatgccgtcg atcacgaggg cctgcagctg
    61261 gtctttcctg gatggtgagc gagatacgct gacagcacgc aaccggtgcc gaccgaccca
    61321 cccggtactg gctcgcgctg cgcgccaatg tgttggttgt cacctaggtg gggagggaga
    61381 gtatgcgagt cctcgaagca cacggcgacc tctgggatgt tcatgctcag ggctcttgga
    61441 tcgccatcac aacaaacggg gtgataagaa caaatgggca tggcgtcatg ggagcggggc
    61501 ttgccaaaca ggccgcggac cggtttccta gcctgccctc gctgctagga acccatctac
    61561 gccgtttcgg caacattccc acatcgattc cctccatgcg gatcatgacc ctgcccacca
    61621 aacatcattt tcgtaccgcg tctgacctcg cccttatcca gaatagcctg cagcacatcc
    61681 agctcattct cacacgtgaa cgcatcgacc ggctgtattg tccgcaccca ggatgtggtc
    61741 tcggtcagct aagctggcag caagtccgag cagcgattgt gcacgtggtc gatcatcgat
    61801 ttctcttcct gcattctact gcttgagttc ctgctcatgc ttccatgagc gtcggccatt
    61861 cgccttcaac agatgtgaga cacgtcccca gctcgcatct cttctcgcca gctagacgtc
    61921 acctcacgac ccatcactgt cttcgtacca gaaacggcct caggcgtgcg tcgcacgcag
    61981 agtggaggat ggtcgcgtac catccattca caagagggag acttgccatg cgtcgtcagg
    62041 cttacgcggc tatctcgttg acgctatttc tcatattgcc cgcatctcct tctcaaacat
    62101 tggcacagac caaaccggaa gctccgctca cgggagcatc gtggcagcaa tgcctcatcc
    62161 actatgccca aaccctcgcc atcgatcctg acctcttcca tgcgatcgtc caaaccgagt
    62221 cggcaggtca cccctgggcc ttgggatgga gcgaccaaac tgggagacga cacagttatg
    62281 ctccacgtcg ccaggaggat gcggtcgcca tgctcctacg tcttctccag accgctccca
    62341 atgttgacat cgggatcgct cagatcagtt cacaacacct gggctggctg gccagacacc
    62401 accaaatcca gtctcgcgat cttctagaac catgtgtcaa tctctacgcc tcgagtttga
    62461 tccttcagac ctcaatccag aagcatggtc gcacctggag agccgttgcc ggctacaacg
    62521 ggtccaccgc gtacattccg gccgtttgga agcatttctg tcagcgtgcg gcagatgctg
    62581 gctgctcctc cggacgctcg acgcctccgc tcccaagtac gccgttacct cctccatacg
    62641 acagagacac cgttgagatg cagcggaata caggtggcac gccgatatcg tggaagatca
    62701 gtccagtcgg caggcagctt tcgtttcaga gacctcacat agaagagacc gcaacacaca
    62761 cttttgatct cctgaacgtc cgcgcactgc tagtcgtcac ccttgctctt ctatgtggtg
    62821 ggattggcct gctcctgatc ctcgcgcttg gtgggcgcct cgtgctcttg gccgtctaca
    62881 tcgttcgaga agggctatat atgcttcgca ggcaacgcgc cgcacatcat cgcctgacag
    62941 ggtagcgtca cgaaacccag cgtgcgctcg cactcaccgg ctcaccatga ggagaggatc
    63001 gcccgtgaag actgctctcg atcaacacca gctcgggatc tacgacgttc cgcgctacac
    63061 catcagtctt atcaaagagg ccgcatcgac gttccacgcc aagactcgat gccagccatc
    63121 ggaagacgtc taccgatact tcaaagacat ctgcgaaaac caagatcgcg aacatcttta
    63181 tgctctcttt ctcgatacaa aacatgtgcc catcgggatg aatcatgtgg caatgggcac
    63241 actcagcacc acgatcgtcc accctagaga agtctttaaa tgtgccattc tcctgaacgc
    63301 ggcttgcctg gtcatcgttc acaaccaccc aagtggcgat ccctcaccga gccccgaaga
    63361 ccgggccctc acgactcgga tcgccgaggc gggccagctc ctcggcatcg cactcctcga
    63421 tcatgtggtg atcggcaacg gtaggtattt ctccttcgcg gaccacggac cactgacgca
    63481 acagactctc acatacacgt cctaagataa ctgcgaaggt tcttccgacg tcccatgagc
    63541 tttgattcga aacgcaacag cgggaggtgt ccgagcggct agttttcgac agactctgag
    63601 cccacagcgt gggttaatct attgaaactc ggatgaaaca ggagggaatc aagatagccc
    63661 cattagatga gaaaactgtc ttccatcggc tggtggagtt gattgccgtt ccttcctctc
    63721 gacgcagcat ttatctaaag agcacaacca tgcatgcacc tatgcgcgat tccgtgacgg
    63781 gacgattccg gcggcgctat tcctgttcct cttgtcagat gcttcgcgtg aatggactgc
    63841 cctgtcatga aaccggctgt ccagatagct ggatagacac gacacgggaa tgccggaact
    63901 gcgggtgcga cttcatacct gattcccggt ggcagcacta ttgcgacgac gcctgcgcgg
    63961 cctggtagcg ctggccaatt cctgctactc aggcccgctc tagcgggcag tgggtgcgtg
    64021 ttcgtgaatc ccaatcgttc tgtggaggct ccttatgggc gtcacaaaaa ccgcgagcac
    64081 gcttgatggt ggcctaaccc caatctgtac ttgttgtggt attgcgctct gttgggacat
    64141 ccctgaagtt gaatacaccg agcgccaagc cttctgggat gcctggcggt gtcaggagtg
    64201 ccatcctgat gccaaggggg cacggcgacg ctggattgag caacaacacg cggctgtcag
    64261 cggcccatcg agggtgccat gacaccttcg atccgttgtc ccttccgacg ccgctctatt
    64321 cccagacatc accccttctg gcccccaaat tccaatcaaa tcgacagcgg gatccgaacc
    64381 atctacgatc tgagcaaggg catcgccctg ttcggggtgt ttgtcccatt ggtgacattt
    64441 aacggggtct ggtataaact ggtgttagcg g
//
LOCUS       DAOQDS010000014        62177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_146_length_62177_cov_21.797849, whole genome shotgun sequence.
ACCESSION   DAOQDS010000014 DAOQDS010000000
VERSION     DAOQDS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 62177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62177
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146_length_62177_cov_21.797849"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PK589_05070"
     CDS             <1..191
                     /locus_tag="PK589_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19000.1"
                     /translation="KGGLVYASRVSMDRATMQVDEKQIHLSPGMAVTVEIKTGQRRVI
                     EFLLSPLLKSMKESLRER"
     gene            244..1245
                     /locus_tag="PK589_05075"
     CDS             244..1245
                     /locus_tag="PK589_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19001.1"
                     /translation="MKQNRRMLSLFVIAGLLSLALIAPTGAGLFGKDETVGERFRKAI
                     EKIKQSCESRKLARGEVCGEVAKLKPADPLATEEGRFAHSIKIPNPVPEDSGYKPGMT
                     SQEYFEHLCKTEAGEFIYKTAENVEGLYMMRPREHANDYMQEHLYAMEDPYGYMDAES
                     QDPEFFFVGPLRYKFFEHPLAPRKKPSWGKSFLDNSYFTAPSDRAYHERYLGSNGKDP
                     LTMIKEFDSVRKARYGYTWRGIVRPHDREMGIAGGELIVLDMETGEVLGVRRGYIRTG
                     SVRNNLTGVWWLTGQACPYYQHRGGRSRDFDFSYWFIGKVVKPINYEMNVRELMNVK"
     gene            1235..8551
                     /locus_tag="PK589_05080"
     CDS             1235..8551
                     /locus_tag="PK589_05080"
                     /inference="COORDINATES: protein motif:HMM:NF018319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNJ19002.1"
                     /translation="MSSNTTAWLNFALQQMAAESYLQDIDLHNELLVKPRLLLGNNNQ
                     FGISPTDADLAGKTRFTSVLADRFLARYDIVDHHASDATGFSATLLFDKETQQYTLSI
                     RSTEYRDEAQGGDWQRDGLPGADGEIKDYGFALAQLVSMERYWRELTAVGGKLAPDAK
                     INVTGYSLSGHLATVFTEMHSDRILQTYTFNGAGRGFVSELGQDGQPVEQTLRRMMLD
                     LEGRLLAFDPEGTQFRSGAAGNIYGDARYDVARQATLTRFPTIGTGNTQFFTIPAGVV
                     GGIPTQSEALDKVVQLVGNAETGSDVQFVANSGIHAPSQSIFIEGQPLLEGFNQQREF
                     QYGNTHSLTLLVDSFALQELFLKVDPTLEQSQIEGIFNAVSAQKADVTVLPGVIPLAE
                     GDTLEKTLDALRKVFLGNVSSTPFGRQPGDFGNLGNRDAFYQNIQQVTAALTGVSYRI
                     DSLVGQSASTMRTIALQDGPNDALGLAYRYALKELNPFVLRGMDRDTTQALYSRHNET
                     GELSLLDPNTGTGNLTSLYFEDRAAFLLKKIEVDSHSISLPSLTHFNDIELGYELGSD
                     ALPLPQVLFGGQGGDSLIGSLLFVDHLYGGQGKDQLYGNGGKDYLEGNQEDDLLDGGS
                     GADTMLGGTGDDIYIVDNIGDVVREYANSGRDEVKSSVTFTLDSQVENLTITESSARN
                     GTGNELENTIVGNSAINILSGLGGQDHLVGGGGNDILLGGTGDNDLLEGGIGFDTYIY
                     HSGDGMDRIEDSDAQGQIVFDARLLQGGIRRAGDGTNTYTSLDGQTTYVMSGTDLIVN
                     GVLIVNENFQSGQMGIQLRDVSAMPRDTGVPAGHFTDTYIGDADAEEWVPGAGAIEVY
                     ANGGNDVLHGDHAPILGAFDDLLDGGAGNDVFFGGYGDDYLIGGYGDDYAFVTDGDMF
                     LGGDGDDYAVGTANYLSFAEPIIGGGAHYADGGAGNDTLMGEMGADVLLGGDGDDVLR
                     GENRPTGWIGLAYDNSFWNRTPQAGIISLSGGNDYLDGGAGSDLLVGDGGDDILLGGA
                     GDDRLYGESDFTLTIAGDDWLDGGEGNDSLFGGAGADMLSGGDGDDLLVGDFSGDPGS
                     ADILDGGAGADELQGGGGDDILYGGIGMDRLAGFGGNDFLDGGADNDELQGGAGDDSL
                     WGGLGNDRVTGQEGDDTLFGDEGDDELQGDAGGDTLFGGEGHDALFGQDGDDLLSGEA
                     GDDLLNGGLGNDQLDGGDGIDDVQGREGDDFLVGGDGSDFLYGDGNNPTVLSLAGGND
                     TLDGEEGDDQLWAGAGQDQLFGGEGSDQLVGDAGDDQLYGEAGNDFLFGDSPFFSTQA
                     GSDVLDGGEGDDTLQGGGGEDDLQGGRGNDLLIGGSGLDTYRFNLGDGADTIQDDGVQ
                     RNRLIFGAGITAESLRLDVGPADTLVLRVGNDEDVIRVSNFGLNDPAEFHTIRQFEFA
                     DGTVLTDGELLARGFQLSSPETGGTLQGTSFDDRMQGSQVGDLLYGRGGNDVLLGGVG
                     DDVLQGDEGDDELDGGAGNDRLYGNQGFNVLRGGEGNDILESVGLGDQLLGGGGDDAY
                     HLRSMGQVITEEVNAGRDTIYLTPTESLTFQAPDNFENVRIEDDVYLDSTVQVNLVGN
                     ALDNELSGSHRLDGQAGNDTLIGTGDNTFVFGPGYGQDVVRTGLQVYAHSGLDQVQFL
                     QGVTPSNLSLERQANDLVVKVTGTADELRVQSYYESPANMVDQFLFADGAIWTSGEIE
                     NRVRTFIGVGADESFYGSAGDDTIRGMGGNDQIRANAGNDVLDGGVGNDFLEGYSGND
                     TYVFGRGYGHDTIDDQGDSADIDTLQLVDGITPGDITLQATPDFQTDALLTIKNSADQ
                     VTLAGFFQSDGMRVDRIQFSDGTMWDYSAMLAHTEGVNLVGTEDTDYFYGNVTNDTLL
                     GVGGDDVLSGGAGNDTLDGGTGADRMTGGSGNDVFMVDNINDIVMESVGQGIDTVYSS
                     LTYTLGSNLENLTLTGSAAINGTGNSLNNILTGNSAANVLTGGAGNDTYVVGLGDTVV
                     EAASAGTDTVRSDISWTLGTNLENLILTGIAAVNGTGNSLANTLTGNSAANILSGLAG
                     ADTLIGAQGDDLYIVDNVGDKITELAGEGLDTVQSSVTYTLSANVENLTLSGTSAING
                     TGNGLDNVIIGNSAANQLVGGAGNDTYVIGSGDTVVEGINAGIDTVQSSVTHTLAANV
                     ENLTLIGTGAINGTGNALNNILVGNNATNTLTGGAGDDTYVVGTGDTVVEALNAGLDT
                     VQSTVTWTLGANLENLTLSGAAAINGTGNTLANSLIGNAGNNVLMGGDGNDTLSGGKG
                     NDVLNGGTGNDLFQVARGDGQDTITDASGSSDRLSFASGINPLDLMLSQSANDLRIAV
                     YGSTDQVTITNWYAGAANQIETVQAGNGQQLMSTQVNQLIQAMAGFTQQTGLSWDQAI
                     AQRPQDVQQILAANWH"
     gene            8832..9326
                     /locus_tag="PK589_05085"
     CDS             8832..9326
                     /locus_tag="PK589_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19003.1"
                     /translation="MSKHRGFCFTLLLLLVGVVEGCTTMPSGTSTVLPSSAFFPLDAS
                     ELKPLQAIAHAQDARMKNCHKGPACEDAYYTRALVALFENRADAITVFQELRTAMPNS
                     RYEVATVGWLNLLQDASLSSDHNKALMVQLKQEVLHNLLDRSEVATARPVKDHDRRVA
                     ELAR"
     gene            9576..10427
                     /locus_tag="PK589_05090"
     CDS             9576..10427
                     /locus_tag="PK589_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ19004.1"
                     /translation="MRLFSVTAVLSAIMLVGVGMASAAEPQAAASGTSAEGIKGKDGA
                     PMVLIPAGPFTMGSNDGLPAERPEHTVTLDAFYIDRYEVSMKHYRSFLVEAKHDAPPT
                     WDDEAAETVGDRPAVGMGWADAKAYCAWAGKRLPTEAEWEKAARGTDGRRYPWGQMQP
                     FVDIANYNRGVWVSEAVTLTSVSGGVEGMSVRHGLKEGGRSPYGLHHMAGNAAEWVAD
                     WYDREYYSKSPEKNPTGPETGEKKVIRGGSWSDLPVALRSSARVSAEPDFQDRAIGFR
                     CAMTPGL"
     gene            10532..11038
                     /locus_tag="PK589_05095"
     CDS             10532..11038
                     /locus_tag="PK589_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19005.1"
                     /translation="MVAPNDSSSVPPSQHNHKAIAALVAGLVLLLALVGIQNAPPPSA
                     VAPAVPNIDPNMVPLVTGEEPIQELFVRAGCVVCHRIPGIVGADGKVGPPLWLGKTGA
                     SRLADPQYRGQAQTVREYIVESVVSPGTYVVPGFPADTMPVWYGRKLTAGALDKIAAY
                     LELATDEP"
     gene            complement(11103..12266)
                     /locus_tag="PK589_05100"
     CDS             complement(11103..12266)
                     /locus_tag="PK589_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNJ19006.1"
                     /translation="MDVRTLLEVMVKQESSDLYLTVDAPPIYRIHGSTHRTDAPPFTN
                     EQLESLALALMRGQQRGEFEEKMEMNLALYYKDLGRFRVNIFRQKGNVGLVFRHIKAE
                     IMTVEQLDLPPIVKDIAMTKRGLVLVVGATGSGKSTSLAAMIDHRNTVHAGHIISVED
                     PIEFVHHHKKSIVTQREVGFDTHSFGNALKNTLRQAPDVILIGEIRDTETMEAAITFA
                     ETGHLCLGTLHSNNANQSIERIMNFFPVERHAQIYLQLSLNLRAIISQRLIPSLDGRR
                     VPALEIMLDTPRIKDLIKRSEIDTLKEAMEQGIDEGCQTFDHVLLQLYKAGKISIEQA
                     LINADSANNLRLKIKLAGLKGDDAVAALLDKNERDEKGFQIQGQMSQGGGKKR"
     gene            complement(12316..13371)
                     /locus_tag="PK589_05105"
     CDS             complement(12316..13371)
                     /locus_tag="PK589_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNJ19007.1"
                     /translation="MIDISKLLTFGVQQGASDCHISAGEPPMIRLHGDLKKLDHPPLT
                     QDETHALIYDMMSDAQRKNFEEHRECDFSFDLGDIARFRVNVFVQGRGLGAVFRTIPT
                     EILPLEKLGMPPILRQLCDREKGLILVTGPTGSGKSTTLAGMIDYLNNTFEGHILTIE
                     DPVEFVHKSKKCLVNQRELGVHTLSFANALRAALREDPDIILVGEMRDLDTIQLALTA
                     AETGHLVFATLHTSSAPKTIDRIIDAFPPNQQAQVRAQLSETLEAVLTQTLLKKKSGG
                     RIAAVEIMVGTTAVRNLIREGKLHQIPGIMQASQKDGMQTMDMALVDLATRGMVTKAE
                     AQSRSMNPNLFNTAGAA"
     gene            complement(13573..14763)
                     /gene="bioF"
                     /locus_tag="PK589_05110"
     CDS             complement(13573..14763)
                     /gene="bioF"
                     /locus_tag="PK589_05110"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNJ19008.1"
                     /translation="MFGLVKARAPMFEKHLQKLDDQHLLRRLRTIASATGPTVTLDGR
                     DIILLSSNNYLGLATHPTVVNAAIEATRRYGAGAGAARLVCGTLTPHETLERTLAGFK
                     GTEAALTFAAGYLANISVIPALIGKDELILADRLCHASLIDGCRLSGATFRVYHHRDM
                     NHLEQLLARRPAGRRTLIVTDGLFSMDGDIAPMKDLVQLAERYGAELYVDDAHGTGIL
                     GRTGRGTLEHCEVESRVPYHMGTLSKALGSAGGYVTGSATFIAYLVNTCRAFIYTTAP
                     PPATAAAATAAIQVIEQEPQRRASLWRNRERLAQGLLRLGFRLAASESPILPVIIGDP
                     DRAMKLAQALLAQGVYAPAIRPPTVPPATSRIRFTITADHTDEQIDRALIAAEQAGRS
                     INLI"
     gene            14847..15683
                     /locus_tag="PK589_05115"
     CDS             14847..15683
                     /locus_tag="PK589_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ19009.1"
                     /translation="MSYRIRVPAKVDPLDEAHLLTGVDRVLHVLQEQRRAVLAGLGVV
                     LVAGAVVAGVMWYDYQSTLKARELDQEATLHYLNRPADDPKKSQEQLSQAIALYKQVV
                     EQYPRSPAAPLALFHLGNAQVLANEVDAGIETYKRFMLLYGSNTSLLGLVQQRLAYAY
                     LAKGDRDQAVKAFTGILDVPGALNKDHVLFELAKIEESQSRPEGALAHYQDLMKNYPN
                     SPFTSEAAVRVKVLEVKKAPETPAAAPAPAPAASAPASQPEQPAKASTGKSAASSSKK
                     KQ"
     gene            complement(15720..17021)
                     /locus_tag="PK589_05120"
     CDS             complement(15720..17021)
                     /locus_tag="PK589_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNJ19010.1"
                     /translation="MQTEPALTSEVPAVPLPDSLQPPAQEPDYLLEEEDRTAAAESEA
                     AEDPAAVASEPSTEIPPAEALLQLKPLSGTTARFADLLTPPAEVIQEAAVEQGSESDN
                     VSEYNVPIVLDPSVQGHIRFFNVAIRNRFEQWLIRLSHYRPLVDSIFSEFQLPSDLIY
                     LSLVESGFNPHAYSRARAAGPWQFMKGTAKVYGLRVDSYVDERRDPVKSTVAAARYLR
                     DLYDLFGTWPLAMAAYNAGEGKVMRALHTAQAESFSDIAKTRLIRRETKEYVPRFMAA
                     TIIAKNPDRYGFPQNDVRPHQFEEVVVRRPIHFKAIANVTGISYQELKVLNPELRRDA
                     TPPDDPEYHLKVPVGTREKVEQLLERAPTHKFPPLPMPVQVKGRHFKNETDSGHWYRV
                     RVGDSLEKIAKRFNISVKTLKSNNNLTGPTIKAGSRLVIAN"
     gene            17436..18581
                     /locus_tag="PK589_05125"
     CDS             17436..18581
                     /locus_tag="PK589_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNJ19011.1"
                     /translation="MLKRYLLAPGPTPVPPEVLLAMARPMIHHRAPEFDKLFAEVRED
                     LKWLFQTRNDVLILAASGTGGMEGSVSNFLSPGDKALTINGGKFGERWTKLCKTFGAQ
                     VTELKVEWGRAIDPQAVADALKKDPSIKAVYVQASETSTAVAHDVKALAEIVKQYEDT
                     ILVVDAITALGVLDLKTDAWGLDVVITGSQKALMLPPGLAFASVSEKAWRLADKAKNA
                     AFYFNFKRERENQQKSTTAYTPAVSLILGLKEVMNILKAEGLEAVFARHAMLATAMRE
                     GVKAAGLSIFPQERPSDALTAISAPEGVDGQAVYKNLRTQYGMTAAGGQDHLKGKIFR
                     ISHMGYIDSFDVITALAAVEMVIKGLGYPVKLGSGVAKAQEIIMGKS"
     gene            18610..20202
                     /gene="serA"
                     /locus_tag="PK589_05130"
     CDS             18610..20202
                     /gene="serA"
                     /locus_tag="PK589_05130"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNJ19012.1"
                     /translation="MKILVSDSLSKQGVEVLEKAGFTVVVKTKLPKEELLKEIKDADG
                     LIVRSGTKVTAELIAAASRLKVVGRAGSGLDNVDTPAATRRGIVVMNTPGGNTVTTAE
                     HTMAMIFSMSRRIPQATASTKGGKWEKEKFMGVELYNKVLGIVGVGQIGGYLTKLAQG
                     VGMQVMAYDPYLAPERAQKMGVSIVELDELFRRADVISVHTPLTPETKSLINAQAIAK
                     MKTGVMIANCARGGIVHEGDLCEALKSKKVAAAAFDVFEDEPVKSDNPLLALDNFICS
                     PHIGASTTEAQENVAVGIAEQIVEYFTKGIARGAVNIPSVSPELLPQLQPYLSLGERV
                     GLLQAQLLEGGLERLTVEYSGEVAGLNVAPLTIAVLKGLLTPILEDPVNYVNAPVVAK
                     ERGIEVKEVKSNDAGDFTSVIRVRVEAGKKSHQVAGTLYHRKDPRIIEIDEFKVEVVP
                     DNHLLLVLNEDRPGVIGTVGHILGDHNINIARMQCSREERGGKALLIFGLDAPLPAPV
                     LDQITNSKHILSVKVADLSKGL"
     gene            20221..21345
                     /gene="hisZ"
                     /locus_tag="PK589_05135"
     CDS             20221..21345
                     /gene="hisZ"
                     /locus_tag="PK589_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNJ19013.1"
                     /translation="MGSLSLKASASSTSLVAGRERSLIPVGMSTILPAAARRIRHLEQ
                     TLLAVLARGGYDEIILPMFEYLDVLAPGLEPELLEKCYQLVDRTTGRLMVLRPDATAQ
                     IARTVAMGMMGERLPLRLCYRTSVFRYEREHAGRDREIFQVGAELIGVDGVTGDAEVL
                     TLLLDCLSQVGLSAFKVAVGHVGFFTALLVRSGLSSEGQKRVEQAAARKDIPLLEELL
                     AREGLARSTGRIILEVLELCGGPEVLSRGRKLVGRDKVLLKPLDRLAQVYERLVSPGQ
                     ESVLIDLGEFRGFEYYDGIVFDVFAPGIGAELGGGGRYDHLMGRFGRTAASSGFALDV
                     DRLFRAIDSSVRPLPPDATRSQSKRRPATPPPSRRPRSRA"
     gene            21342..22697
                     /gene="dnaB"
                     /locus_tag="PK589_05140"
     CDS             21342..22697
                     /gene="dnaB"
                     /locus_tag="PK589_05140"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNJ19014.1"
                     /translation="MKSLSAVDLTAPRLPPQNIEAEQSVLGGILLDNTAMAKAMEVLT
                     DQEFYRTAHRKIYQAMLELSDRGEVIDQITLTECLKGRSELDAVGGSAYLAELVQAVP
                     TAANIRYHSKIVRDKALLRGLIETSTEVITRGYDGTAAVDELLDFAERSVFGLAQGKL
                     DRSFTQVNHIIKESLDVVDKLSKRKERVTGVPTGYIDLDDLTAGLQPSDLIIVAGRPS
                     MGKTSLALGMAQHAALHAGTVVGIFSLEMSKPQLVLRMLSSEARVDSHALRTGRLQKE
                     DWWRLAEAAGKLEQAPIFIDDSGAVTVQQMRGKARRLKAEKGLDLLIVDYLQLMQGKS
                     DSESRQQEISDISRSLKSLAKELNVPVIALSQLSRAVEARKPPVPMLADLRESGAIEQ
                     DADVVMFIYREEVYEPNTERKGIAEILVSKHRNGPIGKRDLFFHDRFAKFENLETRES
                     V"
     gene            22779..24635
                     /locus_tag="PK589_05145"
     CDS             22779..24635
                     /locus_tag="PK589_05145"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ19015.1"
                     /translation="MQRLSEERRQSCTLCKPVGPKAAAVMLIALACVTAACQTAAPSR
                     STQPVVPAAAPPVSRPLSPASDSRASYHFLLGYQAELEQETEQAIKEYQLALQTDPSS
                     HYLKARLAVLYFTAGDVPAAVRFADEVADAPGLDAQMLGQIGGMYAAAGKPDKALRLF
                     NRAIEQEPQRSEHFFAKGLLLANQKQYAEAEETIRSGIKVSPDSAVGYYYLGRIGVEA
                     RDFDKATTHFEQAVTLNPAFEPAYVALGSVYEAKQDRGKAIDIYRRYLQGVNPKNREI
                     RHHLIRLQVSAKQYDEALRELQEMLEEDPSDLDAQLRMGLVYGEQKNYPKAIQQLTQI
                     LTVRPSELKVRDYLGYLYEETKDYAHAIEAYRHNLTLEPSYFEGHLHLGVLLYRTKQY
                     PEAIQHLKEASRLNPKQPEAYIVLGLSHFQVEQYEPSLQAFLEGIRHNPDNADLHFNA
                     GTAYDKLNRFDDVVKSMETTLTLDPHHADAMNYLGYSYAERGVKIEEAIALTKQAVAL
                     RPTNGYYVDSLAWAFFKKGLLSEALAEMKRAVALVGDDPVIYEHLGEIHLKQQHLSDG
                     REALLHALELDPSNDKLMQRFRELGLGDPAKEERIRQAIRRVTERKTAPSAQ"
     gene            25023..25550
                     /locus_tag="PK589_05150"
     CDS             25023..25550
                     /locus_tag="PK589_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNJ19016.1"
                     /translation="MVTESKQAGFTLIELMVVVVIVGILAAFAIPNFLRYRAQAMQAE
                     ARTNLAGIFVGEMSFFTERKEFGNFTDIGFAVAGPGTNRYTYRTGLGLGAGLGPNGGN
                     LCGSAGNCDTIQSEVPAAGAISFTGSVGIATTSSAGFTATAAADLDGDSTHDGWYVND
                     SKQGLSAADPNDVSS"
     gene            25805..26326
                     /locus_tag="PK589_05155"
     CDS             25805..26326
                     /locus_tag="PK589_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNJ19017.1"
                     /translation="MLKQVKGQKGFTLIELMIVVAIIGILAAIAIPNFLRYQAKSRQS
                     EAKTNLGAIFVAETAYLSENSRYGSFSEIGYALAGTTNRYTYRSPAILGNGASSNTVG
                     VDQIPCGAPVGCTVQSDASPAVPSLASSTAGSVGFTASAVASIDNDPTIDGWSVNDIK
                     GGLTQAVPDDVIS"
     gene            26634..28538
                     /locus_tag="PK589_05160"
     CDS             26634..28538
                     /locus_tag="PK589_05160"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNJ19018.1"
                     /translation="MIIRLVILAWLGGTLIQAIRAGRLIVPRLGARYVVLAFLGFAVA
                     ATIFSPYVHQSRQWLVMITGYAILFYLLVSVVDRWERVHTLTMMVIVMGLGEAAWTIV
                     QGVVWNVVRPSGTFFNPNFLAGYLTVSWAVLLSIAVYGYRKSAILYRSSVSPVFWWAG
                     MGSALGIVLIAMIFTQSRGGLVAWLAATVWILTARYGWKLAGMGTAALLLAALIVPTP
                     LKERVLAEHEQNPVSYARWQMWQGAIAQMADHPLGIGLGLYQYTYPLYAFPVEGEITR
                     YGKVAHTPHNDYLQIGVEMGVGALLVFLAAIGLVMRDLLQVLQARLVRWQRSLILGIG
                     GGGIALLVHAALDSSLRESALAILLVLCAGLIVSVARITSGNTDALHAIPIQSRPLWG
                     MGAACLLLLVGAEVSRLGMAWLTFDKASRLAVAGDTEIAIQGFKNAIALDSGKSLYHH
                     GLGSVYARDFEASRDKQLFQLAHAEFKAAIELNPLDSRLLALLAQLYVSAAQTPSLSG
                     RLDEQQIFWLRAALRVYERAVRLAPFSAPYRYEQARLHWMLGERPLAEQQAKEAEQLE
                     PNFLPARALLARLSVDAGRIDDARRELQEIQVRQARYEQWNKNNIDRTFLSVDVAPLR
                     AAVEAKGLAG"
     gene            28544..29500
                     /locus_tag="PK589_05165"
     CDS             28544..29500
                     /locus_tag="PK589_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19019.1"
                     /translation="MRGRTDIRSRLSHVLCVLVLLIGTSGVLHILEGERTAVARAEQL
                     AYLPKGEYFKLAVLGYRQVVADLIWLQAVQHIGAKRDTQLGHTWTYHAVDVLTDLDPT
                     FVPPYQATGLFLGVLVGRHDEGVAILRKGIRHNPANWQLPFLAGYISYYERCDASAAG
                     EFFQLAAHVPGSPAYLPQLAARMTVESGDPTAALEFLDRFSRSVADDRVREALFRRMK
                     EIVQEQDLRFLEESIRRYRLKYGQAPAKVEDLMLHGIIPRLPEDPLGGQYEIDGLTGI
                     VSASSKRDRLRIHEKVACRLGVASTPSTQADAIETLQPAVQQ"
     gene            29513..30484
                     /locus_tag="PK589_05170"
     CDS             29513..30484
                     /locus_tag="PK589_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ19020.1"
                     /translation="MDDIVTHNLTKTYASGWPGRPPFVALDGLSLTVGRGEIFGFLGP
                     NGAGKTTTLKILMGLVRPTSGTALLLGQPAGDVATRRRIGFLPESPYFYDYLTAEEFL
                     GFYGRLAGLNRSAITQRVTDLLELVGLVEARTRQLRKFSKGMLQRIGLAQALIHDPEL
                     IILDEPMTGLDPVGRKQVRDLILSLRDCGKTVFFSTHILHDVEMICDRVGIVMKGRLV
                     ASGRVDELVRQDHTRSVEVVCQQLKVEGNAFIHSLATRVLQQGQQCLIVLPSPDAVDA
                     LVGEIRRQGGQLLSITPHKASLEDLFFQEAAQATTRVLPHMTSGRAS"
     gene            30486..31250
                     /locus_tag="PK589_05175"
     CDS             30486..31250
                     /locus_tag="PK589_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNJ19021.1"
                     /translation="MGAIGVIAVNAFRESLRDKILYNLVLFAGLLIGLSVLLADLSIT
                     EHHKVIADMGLAAINLIGVIIAIFVGISLVNKEIERRTIYTIMARPISRTFFIMGKYL
                     GLALTLFVNLAIMLAVFLLTLWLYHVPIQTSLFQAVALIFVEILVVTAIALFFSTFTS
                     TTLSAIFTLGLYVIGHLTADLRSMVANSESGAVKGVVDLLYYLCPNLEMLNIKGQAAV
                     GLVVAPEYLMLASLYGLLYASLLLTGACLVFQQRDF"
     gene            31367..32191
                     /locus_tag="PK589_05180"
     CDS             31367..32191
                     /locus_tag="PK589_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ19022.1"
                     /translation="MVFQRYGLSPLIVLTSMSAHWNLIRQLTKREIASRYRGSLLGML
                     WAFLHPMVMLTVYTLVFRGAFGMRWGQDGESALDFGLLLFSGLIVHALFAESIHRAPY
                     LIVNHSNYVKKIVFPLEILAWTSLGSALFHALISALVLIAFYGFLHHDLHWTMLLVPL
                     LLLPLSLVTIGISWFLASAGVFVRDIGQASGPLTTVMLFLSPVFYPAGAFPEAYRILL
                     YANPLTFLITQAQDVLIWGRPPSWIGIGLYCAGSYLIAWCGLLWFQKTRKAFADVL"
     gene            32181..33554
                     /locus_tag="PK589_05185"
     CDS             32181..33554
                     /locus_tag="PK589_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ19023.1"
                     /translation="MSSEWAIRVRQLSKTYRVYDRPRDRGKQWILPPLQKCFGQSPSR
                     YYREVHAIEDVSFEVQKGETLGIIGRNGSGKSTLLQLIVGTLSPTSGTIETDGRIAAL
                     LELGAGFHPEFTGRENVFLNATLLGLSQADIDARLDDMLAFADIGSSIDQPVKTYSSG
                     MVVRLAFAVMAHVSADILVIDEALAVGDAFFVQKCIRFLRAFMERGTVLFVSHDSAAV
                     LSLCRTALWLEHGRIAGKGEAKVICDGYLHQPTPMEIPREKVAEGLPSSDPSDPAQPI
                     QNDSSNLAVLPHHETRSASHSRPAWIETITLCNANGRTVHTVTQREQVTLRVCCVTSV
                     GLASPIVGFLVRNRFGQSLFGANTCAAGNAAGHGVSPGTRFVAELEFVMPVLHPGDYA
                     MSIALADGTQEEHVQQHWIHDALAFTSAPSEWCFGLIAVEVMRTQICEIEAQDAQHER
                     LHDSIAL"
     gene            33523..34257
                     /locus_tag="PK589_05190"
     CDS             33523..34257
                     /locus_tag="PK589_05190"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ19024.1"
                     /translation="MNASMIPLPCKVCGGSTFSHHDVLWGDLIAQWGLSSREVDYINV
                     QQGTACTGCGSNVRSIALADALLRWTRATTTLQEWVHTPVARRCDVLEINEAGSLTGT
                     LSTLPGHRLVRYPEYDMMALPFEDRSFDVVLHSDTLEHVPDLMKGLRECRRVLRPGGA
                     CLFTVPVVVGRLTRGRKGLPLSVHGSEGCIDTALTVHTEFGADVWCQVLESGFDECRI
                     VPYRYPAGIALIAEVAESRPVTGGQW"
     gene            34254..35201
                     /locus_tag="PK589_05195"
     CDS             34254..35201
                     /locus_tag="PK589_05195"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ19025.1"
                     /translation="MNKTVNESTELEATGERFLPEMQGTIALEHLHRYALAKDCAAGK
                     IVLDIACGEGYGSAVLAGTADRVYGVDIAPDAIAHARAIYQRPNLEYLVGECANIPLP
                     DCAVDLVVSFETIEHHTEHEAMFAEIKRVLRPGGVMVMSSPDKLEYTERSGHANAYHV
                     KELYAEEFQRLVERYFNRVAMLGQRVLYASAILPQGAPGEAVHYSRDKDSAIQAASGL
                     VRPCYQVAIASDAALPPVLSSLFEQAGESDPEIHALQHQLEHAGRRILGLEEEVAGLK
                     AAIELMMNSHSWKATRPLRAVANALRAVSGNRVEKSRRA"
     gene            35294..38356
                     /locus_tag="PK589_05200"
     CDS             35294..38356
                     /locus_tag="PK589_05200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ19026.1"
                     /translation="MNRLMQRLIKAIRFSRFAIVEGLRWYKRHGRLPRKAECADLFRK
                     AFFRFKILAQRISPAPKTIEPVLPPYDAWLKVNVWNRRMRDDLLDRLSRHAGRLPTIS
                     LVMPVHNPPLECLTKAIDSVRAQVCGEWELCIADDCSTNPAIRDELERFRAEDPRIRL
                     TYLERNLNISLATNRAADLATGEFLLFFDHDDELTPDAVGEAALYLAEHPEIDALYSD
                     DDKIDSAGKRYAPQFKPDWSPELLLSYMYMSHIFVVRRSLFETVGAMRQGFEGAQDYD
                     LALRVAERTGMVGHIPKVLYHWRALPGSTASSGAAKPASIEAGRRAVAEAFERRGIAV
                     RVTQPEFAAAGNLGIYCHEFPDDGPTVTILIPTKNQVTVLRQCVESLRATTYRQYEIV
                     IIDNESDDPETLAYLHSLPHRVIRLGNPSGRFNFAAINNRAVEQVTGEFILFLNNDTE
                     VKSPRWLSQMVGYAQMPGVGAVGAKLIFADGRIQHAGVIHGLYHGMAGPAFKLTPSWE
                     HGYLAYASVVRNYSAVTAACLLTSRRRFLDVGGFNEAEFGVAYNDVDYCYRLVDQGYR
                     CVYCPDAQLMHYEGYSRGFRDDPAEVAAFKQKYKRRQDPWYSPHLSLVDESFNLVPRA
                     VAARRQKPIPMVMTALSLNCEGAPWSQYELTKELVRRKVIQPIVFSPLDGPLRSLYEE
                     EGIPVMVDRHPLWGVTTLKEYEEAVKVFSSKCLSWGAELIYANTLQSFYAVAAAQIAG
                     IPSVWNPRESEPWQTYFDYLPDGVIQKAYDCFASPYRVVFVADATRDAYAALNTRHNF
                     TVVRNGLDQTRIQQTSEEWLPAQARQSIGACDGEVVILLLGTVCPRKGQQDLVKALAQ
                     LSPDLQKRVRCYIVGDRPSEYSAGLHALVAQLPSELRSRMHLVPETRQTARYYRAADL
                     FVCTSQLESYPRVVLEAMAYGLPIVTTPVFGIREQVREGVNALFYEAGDSGALADRLR
                     RLLQDEALRARLASKSASVLALGTDFDEMVSSYAEIFAEAWLSGGPLS"
     gene            38353..39492
                     /locus_tag="PK589_05205"
     CDS             38353..39492
                     /locus_tag="PK589_05205"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ19027.1"
                     /translation="MTVRFNLETPGGPQLNTINRFSGWYIPDHGRRPELYVACNGKRA
                     ASLEWGSVRPDVSSVYSTHSQAIISGFQGDLLMGEQEQAQAVEVVVLDAAGQDRELFR
                     RTYAVGPYRPPVIRDRSFNLFELLVCPECRSPLAKQDQGWMCPRCARLVPLKGGVPHF
                     LQEQGTPCLHVTEQTNTHPYSADVLDILGRHRAGLVLDFGAGHTPKELLRPHVVYLDA
                     VQYEWTDLVCTRARLPFRDHSFDAVVSQAVFEHLEDPHYTARELCRILRPGGIIHLDT
                     AFMQPLHGDPWHFFNMTQHGLRKVMAPFEEIRCGIKSYQHPSASLLMQFDAIAPFITS
                     RKWRNTIAKWRKELEAGAGEFDDALGEAGRTTLAAGFFFEGRRPQ"
     gene            39618..40661
                     /locus_tag="PK589_05210"
     CDS             39618..40661
                     /locus_tag="PK589_05210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ19028.1"
                     /translation="MEQRLRSMLLPGMRRLGLVPLLRRLRAGFRSAGNAEPAGSGHPG
                     NDHDRVSDYWGQQFQAMRVDNSYWLNNKLVEEATYRLMTDTPRHWLGWLLNDYFAQRT
                     FDRSLSVCCGDGAHEIQLYTSGKVRFVSGVDISEGAIKQAAARFAEAGAPADCYRFEV
                     RDVNALQLEDQYDLIFSTGALHHTTNLEALLSTVERALTPNGYFVVVEFIGPNRFQWT
                     DRQIEIANQVLNVLDPAYLRDGRNTRFERPTIENMLACDPSEAVRSADVYPLVKKYFD
                     VRYERFYNGTLLHQLYPLLRTELANQGKRDFDSIVKLILLLEDLLIKGNVLPSDFVFL
                     ICQKPVPGSRRAA"
     gene            40678..42504
                     /gene="asnB"
                     /locus_tag="PK589_05215"
     CDS             40678..42504
                     /gene="asnB"
                     /locus_tag="PK589_05215"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNJ19029.1"
                     /translation="MCGIVGVCDRMEPQQAHHVLSRMLATMIARGPDGEGRFVDPHIA
                     MGMRRLAVIDVEGGGQPLFSRDGQVVAFQNGEIYNHAALRRELAQRGVECRTASDTEV
                     LAHGYDHWGMDGLLARLDGMYAIAILDQRNHQLHLARDRFGEKPLYYSVANGKFAYSS
                     NTTALAWLPWGDRDIDAGSVDRYLALHYVPGRRTMFKGIQRVLPGEHISISLDSLQII
                     HDRYYRPAMGPIEPVDTGTLVGRIEAAVTSRLVADVPVGVFLSGGIDSSIVAALAAKH
                     HPAIDTFSMGFKDARYDESPHAQAVARAIGSSHHHFTFDETAFHDLLPSVIDGLDEPL
                     GDQALLPTYWLCREAKRHVTVVLSGEGADELFAGYEYYGALLSRRSWRACLKAWVMGR
                     GPADELQRLCDNVWPVTPSGFPLVMDTAGRRQLLGGYAPDRDPWEEALRSTLQQSANG
                     LQRATLADVLTWLPDDLLVKLDRMAMANSLEGRAPFLSPVVAETALRLPESSRMTPVR
                     SKVALREVAARLLPPEIVHRRKQGFVLPMATWLKQWLRRVENLYEYFDLQRIAVFDGN
                     AAATLVERQLTSDRPNERLLFSLVMLAEWHHSFFRRIPPAKF"
     gene            42676..43815
                     /locus_tag="PK589_05220"
     CDS             42676..43815
                     /locus_tag="PK589_05220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ19030.1"
                     /translation="MTVTYFSKSSSIGPSSRYRVYQYLPHLYNAGIECHVEPLFGPTY
                     FSILHIRSLMLQTVLKIPYVVARFLKRLGTLMMLGQRDLIVIEGQLFPYAPPLVERLL
                     CWLGYRLVIEMDDAIYLTRGHERKMPVLFRMATGVIVGNDRLAAYATQYSSKVTVVPT
                     VVDTERFAPRPLRPTAASHLLDDPITIVWIGLAYNLKYLNVLAPAIRRLQERYRVKLR
                     VVCSQPPVLDGLDVEFRSWEWRREVEDLQDATIGVMPLEDTEWARGKCALKLLQYLAV
                     GLPAVASPVGVNRDILVNGDNGFLASTEEEWYLRLESLCRDPQLRARMGQAGRRTVET
                     RFSLAVWGPKLADVYRAFAEDAPSRQMPRPVGLSVRGHGHSTGMR"
     gene            43812..44870
                     /locus_tag="PK589_05225"
     CDS             43812..44870
                     /locus_tag="PK589_05225"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNJ19031.1"
                     /translation="MILLALTFLVAVLLSVYGVPIARRAALKFGIVDSPDGRLKHQGE
                     PVPYLGGLAIYLSFLVSLAFTFEFRQDVLGIVLSGTLIVMLGLIDDFGVLSPGTKLAG
                     QFLAVFVLIKSGIRIEIASLPDWVDILLTVFWMIGIINAFNLLDIMDGLSAGVGVISA
                     AFLCVVAILNGDQTIAFMLAALMGSLLGFLRYNWRPASIYMGDSGAMFIGLMLGALSM
                     IGKYTQGHSVSLLTPVLILGMPIFDTLFVMYIRFLRGLPVFLGSPDHIAIRLRHWGLS
                     VTHVVLLSYLGAAILGGIGLLVMAVPQDLALILSGVTVLCLAAAAVALTRVNVSSGVV
                     AVSPEAVPARSTERTGAV"
     gene            44867..46177
                     /locus_tag="PK589_05230"
     CDS             44867..46177
                     /locus_tag="PK589_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ19032.1"
                     /translation="MIVIVGAGLAGLSAAYHLRGMPYKILEREREVGGLCRSYVKDGF
                     TFDYTGHLLHFRQTAIKALVESLLPDRLQRHARKSFVFSHDTYTEYPFQVNTYGLPPE
                     VVRECLLGFIATLSRSPSTPPAENPSFKQWIIESLGEGIAKHFMVPFNEKLWQVPLDE
                     LTSDWVSWLVPKPDVKDVVSGALGIKDKAFGYNPSFQYPVSGGIKVLPEAFLPSVENL
                     TYGSELVEIETGRRRAVFRSAQGERIEEYDRIISTIPLPELVRRCVDLPASMRELAGA
                     LRWVSVYNVNLAVAREHVSDKHWIYFPEHRYPFYRAGFPMNFSPSMGQPGCSSLYVEI
                     SHQPTEKESETSLIERVRRGLEGAGVLQPTDELVMSDVKDLYYAYVLFDRYRSRAVKE
                     LLAELERRGISSIGRYGLWEHTSMEDAIAQGQQLAMRLRMRAAA"
     gene            46191..47381
                     /locus_tag="PK589_05235"
     CDS             46191..47381
                     /locus_tag="PK589_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ19033.1"
                     /translation="MDVICHVITKLELGGAQEVAMRVVSGLDRRRFRPVLIAGPGGLL
                     TEEAHALEGVDVRLIPSLVREVHPLQDLQALFELVATFRRLRPKIVHTHSSKAGILGR
                     LAAWLAGVPCILHTIHGYGVTPAQPLWLQHLVIKLEWMVGRVTTHWIAVSQADRRQGL
                     EWELFSASKVSVVRPGIDPTPFAARIEQAERDRLRAALGVGPEQLLVGTVSCLKPQKS
                     PEDFIRVAALVCQRMPAAKCVLAGDGALRPQIEAMIQAQGLQDRVSLLGWRRDVASLL
                     KAFDVFVLTSQWEGLPCAILEARASRIPIVATRVGGSAEAIIEGIQGTLCPAGDVKAM
                     ASRVCQILGDERLRADLRNGAQELPEEFTIQETIKQYHSLYTYLLHATRPAEDVMKLQ
                     PNRL"
     gene            47402..48520
                     /locus_tag="PK589_05240"
     CDS             47402..48520
                     /locus_tag="PK589_05240"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNJ19034.1"
                     /translation="MNIPLLDLKAQYQSMRSEILAAIEATCDEQGFILGPRVVALEQA
                     VATYVSSTHAVGVASGSDALLLALMALGVKAGDEVVTVPFTFFATAGAISRLGAKPVF
                     MDIRPDDFNMDPTLLERVITKRTKAIIPVHLFGQCADMGAIHDIARRHKIGVIEDACQ
                     AIGAAQNGKRAGVLGDLACFSFFPSKNLGGFGDAGMVTTNDKELAESIAMLRVHGSRV
                     RYVHEAIGINSRLDALQAAVLLVKLQRLDQWADGRRRNAARYHQLFTDAQLTDHVTLP
                     VTHPHNFHVFNQFTLRVRKRDELRAYLKDHGVGTEVYYPLPLHLQNCYRDLGYQKGAF
                     PQSERAAEEVLSIPIYAELTDEQLQYVVQMIAAFFRKR"
     gene            49038..50870
                     /locus_tag="PK589_05245"
     CDS             49038..50870
                     /locus_tag="PK589_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ19035.1"
                     /translation="MTIPSSYFLGMSRSSYSILGVALALIAVIGAIDWWLPLGLTITT
                     LYVVPVLIASRIPYPRLTFWVAATASFVTILDLFHSPLYALTWVSAVNRAFALMVIWV
                     TALLCLRRQRDEAELLRINEDIEQQVQERTSDLAAANQELEVLRAEAVSELVAIVKSS
                     DDAIVGMTLTGIIQSWNRGAERVYGYSAEEVLGRPISVLCPPNRLDEVPTMLDRIARG
                     EHVRNVEMVQRRKQGERIDVSLTISPVKDSDGCVMGASAIARDVTVKKRIEAALRESE
                     ARFRMMADTAPVMVWMAGPDTHITFINKRWLEFTGRTMQEEIGDNWFTGIHVDDLERC
                     RKSYLDAFKSEQPFFLEYRLRRHDGEYRWIMDTGVPLFDEDGRFGGYIGTCMDLTERK
                     DMEDQLRRMLKEKESLLREVHHRVKNNLQVISSLLNLQSASIKDPVVNQLFRECQVRI
                     TSIALLHETLHRSNDLSRIKMGDYTRTLTGHLFRSYGVDPGLISLELNVDDVEFDIDT
                     GLTCGLIIDELVSNCLKHAFVESGRGTVHIDLLDHVDGTFTLCVSDDGIGIPTDGVLN
                     NPDSLGLELVTLLAEKLDGHTELRSGSGTEWRIRFQQLQYSERV"
     gene            50875..51870
                     /locus_tag="PK589_05250"
     CDS             50875..51870
                     /locus_tag="PK589_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ19036.1"
                     /translation="MDAASILIVEDEPVVAKDIQLSLQRLGYRVPATATSGEEAIRKA
                     GDTHPDLILMDIVLKGKMDGVETALQIQRRQDVPVIYLTAYADNHTLERAKVTSPAGY
                     MLKPYQANELRTTIELALHRAQHDRHMRARLRWIATTVRCIGDGIVTTDRGGRVTYMN
                     PAAEEFTKVTQDEAMGMSVTALMGFPDDGATQHGGNPANQAMAEMRLVTIDEATVMSR
                     QGEPRSIRGSVAPVVDDGGAVLGAVFVFHERVPGERHLGPSNVQAEAIWRMEERLGRP
                     QGIINLCSWCKRVPDQSGEWYDLATFIAERSAIQFNGGLCPECMDQCFPCNGKHK"
     gene            52030..54048
                     /locus_tag="PK589_05255"
     CDS             52030..54048
                     /locus_tag="PK589_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNJ19037.1"
                     /translation="MTNPSPNTPVSGPLRIALIGAGRHAQHHARAILRCPGVQLVAVA
                     DPSDAAQAAMRDIVPGIGCFKTPEELFASEKLHVVHIITPPATHAPIARMALKAGCHI
                     YVEKPFTESVEDAQQILDEASAKGLRVCAGHQLLYEPPTRVLTQYLRSIGRVVHVESY
                     FSFRTVRHAPGGRKVLRADHQLLDILPHPVYLLLQVLEQAGEGRTELLSLEVSQAGTV
                     HALVRRGGVTGTLIVTLEGRPVESYLRVIGKNGSLFADYVRSTTQRAIGPGSSGIDKL
                     FAPYRQAWQLLTGTTSAMASRFLKSQRSYPGLAELFSAFYESARTGNPSPLSPESLLE
                     TVRICERVAKALKVGEAKALAAAAPKPVESRGVLVTGGTGFLGKEIVRALLSRGRPVR
                     VVARREPSPWERIAGAEYVVADVATGAAAHLFKGVDTVIHAAAETAGGWPEHQRNSLD
                     ATEHMLRGAAAVGIKHFVHVSSLAVLAQGTGRPIPDDHPLEPDSKGSGPYVWGKLESE
                     RLAVQLGKDLGVSVKVIRPGALVDYRDFDPPGRLGKRLGNIFVAVGSPGDRLGVVDVG
                     FAGRFLGWMTDAWDMAPSPLNLLDPVSPTKRELLDRLRQANPDLSVFWLPRFVLIPLS
                     WVATLAQKVLRPGKPAIDVAKVFSVLPYDTSGIATLAPRVDSASELHR"
     gene            54277..55539
                     /locus_tag="PK589_05260"
     CDS             54277..55539
                     /locus_tag="PK589_05260"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ19038.1"
                     /translation="MNMRSNALAFTIGLAILGTLSGCAEDHRWRAAMNDGNTAMRSGD
                     YTHAEESFVAARKEAEVLDPQGKRLAETLSQLGEVNRELGRYPRAEALFQEALAIRER
                     VYGPAHAETAASLTDLGELYRLQGLYTQSEALHQRAREIREKVFGPDHSKTAESLNNI
                     AVVYQDQRRFADAEPVLQRALAIFEKQLGPEHSLTAITRDNLAKMYQAQGQHPRAMPL
                     YQRALTIHEKAFGPNHPIVARNLENLGDTYRAQNQYPQAEALYQRAVSIFRKSIGSDH
                     VDTAEAMSRLGQLYELQGLYSQAEPLFQQAIAIREKQQGADNPQVAGELKNLASLYQS
                     QNKLDQSEDLYKQALVIYEQAVGYDREAYVKTLKSYASLLRRRQRSGEAERLEERAKS
                     VLKRLSLESQSQGKTAADLPLAPVSPVP"
     gene            55746..56498
                     /locus_tag="PK589_05265"
     CDS             55746..56498
                     /locus_tag="PK589_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19039.1"
                     /translation="MNRMVIAIVAVACLVNAACVSKPPKKQFQRLAGEFVSPVHITKP
                     VDQQSAVIGITIKSSAWVPNTEDRLYLVKIENDRDLFEGAKLIPTSIVSPPPGAGGGG
                     TVYVQNVPPGRYAAVAFSTSEKGFGKVREVTLFLLPKSVVKQSDTTVTAGSMHFMGEY
                     EVGASTMVLQENAADEVQAHYFEAFWGKPLAQVIADLQVLGTPAYFAVHTVNVKGGSR
                     DADAEARFQATAKRDLEPSWAPVIERARVPKK"
     gene            complement(57205..57597)
                     /locus_tag="PK589_05270"
     CDS             complement(57205..57597)
                     /locus_tag="PK589_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNJ19040.1"
                     /translation="MAVAHVVRPRSRHQAFRSIRRQLLQDQRILLRQAASWRPPETEA
                     ELPADVLDIATCDRDLALGDLMRQRTYAKLQQITRALNRLDHASYGMCHLCRTDIPLP
                     RLRAQPDATLCVDCKAECEHRAMLRKQV"
     gene            complement(57732..59876)
                     /gene="ppk1"
                     /locus_tag="PK589_05275"
     CDS             complement(57732..59876)
                     /gene="ppk1"
                     /locus_tag="PK589_05275"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNJ19041.1"
                     /translation="MALRSTASHIEPQSATPAPKVSSVRPVTSDLSRPEWFINRELSL
                     LEFNRRVLDLAKDPKVPLLERLKYLCIVSSNLDEFFEVRVAGLKEQVTHGVEQRGADG
                     LTPSELLARIAVLTHQLVQEQYVVLNQSLIPKLADERIRFLKRADWMPSHIRWMRRFF
                     SRELLPLLSPVGLDPAHPFPKLLNKSLNFLVTLEGTDAFGRPNGTAIVQVPRSLPRVI
                     HLPVRNAAWPHDFAFLSSVIHAFVHQLFPGMHVTGCYQFRVTRNSNLFVDEEDVDDLR
                     RALEGQLPDRRFGDEVRLEVADNCPPDLVYFLREQFHLDARDVYQCHGPVNLHRLMAV
                     PDLVDRPDLKFQPFTPGVPTTPVPSEDWFDAIRQSDILLHHPYQSFAPVTEFLRQAAT
                     DPHVLTIKQTLYRTGADSAIVQSLVDAARGGKEVTVVIELRARFDEEANIELAHDLEE
                     AGAHVVYGVVGLKTHAKMSLVLRREGRLLRSYTHLGTGNYHARNARLYTDFGLLTCDA
                     AIGRDVRTVFQQLTSLGQPGRLKHLLQSPFTLHTTLLKWIARETDRARQGKPAHIIAK
                     MNALLEPQIIRALYKASQAGVKIDLIVRGPCALRPGIAGLSEHIRVRSIIGRFLEHSR
                     IFYFLNDGDDRVFLSSADWMDRNFFRRIEVAFPVLDPILRHRLIEEGLRPYLEDNTHA
                     WILHRDGTYKRQTPGRRAARSAQQWLMNRLVT"
     gene            complement(59976..60881)
                     /locus_tag="PK589_05280"
     CDS             complement(59976..60881)
                     /locus_tag="PK589_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19042.1"
                     /translation="MALRSRWSLAAALCAAACLLGPIKPHAGEPPQALCADWKSHHPE
                     WIWCDDFEIDRLSHYFEYDIRHGRFVREAGAGVGASTGMRAEFLPGDPHGGSLHLAFG
                     KTPSEYMKPVDAGTANYRDIYWRFDLRLQPGWTGGGADKLTRATILSSNRFAQAAIGH
                     LWSGAHPGSDPDRLYLDPASGTDEFGTLLTTTYNDFPRLRWLGAAGGQTALFNPAHIG
                     TWFCIEMHMKLNSRDAADGVLEYWIDGQLEAQRTNLNWIGMYNEYGINAVYLEQYWTS
                     VPFSKVQQRYFDNFVVSTARIGCAR"
     gene            complement(60884..>62177)
                     /locus_tag="PK589_05285"
     CDS             complement(60884..>62177)
                     /locus_tag="PK589_05285"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNJ19043.1"
                     /translation="TIPGTVMALCRLNVPSLMIYGGSIMPGQFQGHDVTIQDVFEAVG
                     KHASGKMTNAELKDLEDHACPGPGACGGQFTANTMAIAFEFLGMSPMGRNGVPAMDVR
                     KDDVAFECGKMVMDLLKKDIRPKQIITRRSIENAIAAVATTGGSTNAVLHLLAVAREM
                     GVRLTIDDFDKINRKVPLLADLKPGGHFTAADLYAAGGTTLVAKRLIDAKILHPDQIT
                     VSGRTIGEEAKAATEKPDQQVLRPLSKPIKPTGGLVILKGNLAPEGCVVKVAGHSILH
                     FSGPAKVYEREEDAFKAVQAGKIKAGDVVVIRYEGPSGGPGMREMLGVTAAIVGAGLG
                     DSVALLTDGRFSGATHGLMAGHVAPEAIKGGPIAAVKTGDVITFDIVKRRLDVNITQK
                     EMASRLKKVKHPAPRYLSGVMGKYARHVSSASEGAVTS"
BASE COUNT        12903 a        16373 c        18917 g        13984 t
ORIGIN      
        1 aaaaaggcgg tctggtctat gcgagccgcg tgagtatgga tcgagccacg atgcaggtag
       61 acgagaagca gattcacctg tcgcctggca tggctgtcac ggtggagatt aagactggac
      121 agcggcgggt gatcgagttt ttgctgagcc cgttattgaa gtcgatgaaa gagagtttgc
      181 gggagcggta ggcaaatcca tgcacaaata atggtgcatg aaccttcagg aagagggttt
      241 ctcatgaagc aaaacagacg aatgttaagc ctattcgtga ttgccggcct gttgtcgctg
      301 gcgttgattg cccccaccgg cgcggggctg ttcggaaaag atgagacagt aggggagcgg
      361 ttccggaaag ccatcgagaa aatcaagcag agttgtgaaa gccggaagct cgcccgaggg
      421 gaagtgtgtg gagaggtagc caagctcaag ccagcagatc cgttggcgac cgaagaggga
      481 cggtttgctc attcgatcaa gatcccgaat cctgtgccgg aagacagcgg atataagcca
      541 gggatgacga gccaagagta ttttgagcat ctttgtaaga cggaagccgg agagtttatt
      601 tacaaaacag ctgagaacgt tgagggactc tacatgatgc ggccgcgtga gcacgcgaat
      661 gattacatgc aagagcatct gtatgccatg gaagatccat atgggtatat ggatgcggaa
      721 tctcaggatc cggaattctt tttcgttggg ccgcttcgtt ataaattctt tgaacaccct
      781 ctggccccaa ggaagaagcc tagctgggga aaatcatttc tagacaattc gtatttcacg
      841 gccccgagcg atcgggcgta tcatgaacga tatctaggat cgaatgggaa agatccacta
      901 accatgatca aggagttcga ttcagtgagg aaggcgagat atgggtatac atggcgaggt
      961 atcgttcgtc ctcacgatcg agaaatggga attgctggtg gcgaacttat tgtattggac
     1021 atggagaccg gagaggtctt aggggtaagg cgtggatata ttcgaactgg gagcgttcgt
     1081 aacaacctca caggagtgtg gtggctcacc ggtcaagctt gtccctacta tcaacacaga
     1141 ggtgggcgca gtagagattt tgatttttct tattggttta tcggaaaggt tgttaagccg
     1201 atcaattatg agatgaatgt tagggagtta atgaatgtca agtaacacga cggcatggct
     1261 caattttgct ctccagcaaa tggctgcgga gtcctatctt caagatattg atctgcacaa
     1321 tgaactcttg gtgaaaccac gtttattact gggcaacaat aaccaatttg ggatcagtcc
     1381 aacggacgct gatcttgccg gaaaaactcg gttcacaagc gtattagcag atcgatttct
     1441 tgctcgatac gacattgtcg accatcacgc cagtgacgcg actggattta gtgccacgct
     1501 tctattcgat aaagaaacgc aacaatatac tctttccatc cgaagtaccg agtatcgtga
     1561 tgaggcccaa ggtggagatt ggcagcggga cggcctgccg ggggccgatg gcgagattaa
     1621 ggactatggt tttgctttgg ctcagctggt gagtatggag cgttactggc gtgagctaac
     1681 tgctgttgga ggcaaattag ctcctgacgc caagatcaac gtgaccggtt attctttaag
     1741 cggccatctg gcaaccgtct tcaccgagat gcattcggat cgcattctgc aaacctacac
     1801 cttcaatggc gctggacgcg gtttcgtgtc cgaactgggt caggacgggc agccggtcga
     1861 gcaaactctt cgtcggatga tgttggatct tgaggggcgt ctgctcgcat ttgacccaga
     1921 gggaacacag ttcaggagtg gggcagcagg aaacatctat ggcgatgctc gctatgatgt
     1981 cgctcgtcag gccactctga cccgatttcc aacaataggc acggggaata ctcaattctt
     2041 tacaattcct gccggagtag tgggcggtat cccgacgcag agtgaagcgt tagataaggt
     2101 tgttcagtta gtcgggaatg ctgagaccgg cagtgacgtg cagtttgtgg ccaactcagg
     2161 gatccatgcc ccttcccaaa gtatatttat tgagggacaa cctctgcttg aggggttcaa
     2221 tcagcagaga gagtttcagt atgggaatac gcatagtctc actcttcttg tggattcctt
     2281 tgcccttcaa gagctattcc tgaaggttga tcctactctt gagcagtcac aaattgaggg
     2341 gattttcaat gctgtttcgg cgcagaaagc cgatgtgaca gtgttgccag gtgtcattcc
     2401 actagccgaa ggtgacaccc tggagaaaac gttagatgca cttcgcaaag tctttcttgg
     2461 aaacgtttca agtaccccct ttggtcggca acctggcgac tttggcaatc tcggaaacag
     2521 ggatgccttc tatcaaaaca ttcagcaagt cactgccgcg cttactggtg tcagttatcg
     2581 gattgactct ttggttggcc agtctgccag cacaatgagg acgattgccc tacaagatgg
     2641 tccgaacgat gccttagggc ttgcgtaccg atatgcgctg aaggaactca accccttcgt
     2701 gttgcgagga atggatcgag atacaacgca agcgctctac agccgccaca atgagacggg
     2761 tgaactttcg ctgttggatc cgaacaccgg tacgggaaac cttacatcgc tgtatttcga
     2821 ggatcgggcg gcgtttttgc tgaaaaagat cgaggtggac agccattcta tcagtctccc
     2881 atccctaaca cattttaatg atattgagtt ggggtatgag ctgggttctg acgctcttcc
     2941 cttgccccag gtactgtttg gcggacaggg aggcgattca cttattggga gtctcctttt
     3001 tgtagatcat ctatacggtg gccaaggcaa ggaccagctc tatgggaatg gtggtaaaga
     3061 ttatctggaa ggaaatcaag aggatgatct ccttgatggt ggaagcggtg ctgatacgat
     3121 gctgggtggg acgggagacg atatctatat agtcgataac atcggcgatg tggttcggga
     3181 gtatgcgaat agtggcaggg atgaggtcaa aagctccgtc acgttcaccc tcgactccca
     3241 agtcgaaaac ctcacgatta ccgagtcaag cgctaggaat ggaaccggca atgaactcga
     3301 gaacacgatc gttggaaaca gcgcaataaa cattctgagc gggctcggag ggcaggatca
     3361 tcttgtaggg ggcggcggaa acgacatcct ccttggcggc accggcgata acgacctctt
     3421 agaaggcggc ataggattcg acacctatat ctatcattcc ggtgacggaa tggatcggat
     3481 cgaagacagc gacgcgcagg ggcagattgt ttttgacgcc cgcctgctgc agggtggtat
     3541 ccgccgtgca ggcgatggca ctaacaccta tacaagcctg gacggccaaa ctacctatgt
     3601 catgtcaggg accgatctga tcgtcaacgg ggttctcatt gttaacgaga atttccaaag
     3661 cggtcagatg gggattcagt tgcgggacgt gtcggcgatg ccgcgcgaca ccggagttcc
     3721 ggcaggacat tttactgaca cctatatagg cgacgcggac gcggaagagt gggttcctgg
     3781 agcaggggcg atagaggttt atgccaacgg tggcaatgat gttcttcacg gagaccatgc
     3841 gccgatcctt ggcgcgttcg atgatctgtt ggatggcggt gctggtaatg acgtgttctt
     3901 tggcggatac ggggacgatt atttgatcgg cggatatggt gacgattatg ccttcgtgac
     3961 cgatggggat atgtttcttg gtggcgatgg agatgattat gctgtcggaa cagcaaacta
     4021 tttgagtttt gcggaaccga tcattggcgg tggggcgcac tatgccgatg gcggtgcggg
     4081 taacgacacg ttgatggggg agatgggggc ggatgtgctg ttgggcggtg acggcgatga
     4141 tgtgcttcgt ggcgaaaatc gtcctacggg ttggattgga ctggcgtacg acaactcttt
     4201 ttggaatcga accccccaag cagggatcat ttcgttgtct ggcggcaacg actatctaga
     4261 cggaggggcc ggcagtgact tgctggtcgg cgatggcggg gatgacattc tgctcggcgg
     4321 agccggtgac gatcgtttat atggcgagtc agatttcact ctaacgatag caggtgatga
     4381 ttggcttgat gggggtgagg gaaacgattc gctcttcgga ggagccggag cggacatgct
     4441 ctctggaggt gatggggatg acctcttagt cggtgatttc tccggcgacc ctgggtcagc
     4501 ggatattctt gacggtggcg caggagcaga cgagcttcag ggaggcgggg gcgacgacat
     4561 tctctatggt ggaatcggta tggatcggct cgccggattt ggcgggaatg atttcctcga
     4621 tggcggcgct gataatgatg agttgcaggg aggcgcaggg gacgattctt tatggggcgg
     4681 gcttggaaat gaccgtgtga cagggcagga gggagacgat acgctctttg gtgacgaagg
     4741 ggacgacgaa ctgcaaggag atgcgggagg cgatactctt ttcggtgggg aaggtcatga
     4801 tgcgctgttt ggtcaagatg gagatgatct tctttcagga gaagccggcg atgatttgct
     4861 gaatggcgga ctcggcaatg accagctgga cggtggcgat ggcatcgatg atgtgcaagg
     4921 acgtgaagga gatgattttt tggtcggcgg tgacggcagt gattttctat acggggatgg
     4981 gaacaatcct actgttctga gtcttgcagg tggaaacgat acgttggatg gggaagaggg
     5041 cgatgaccag ctttgggccg gggcgggaca ggaccaattg tttggcggag agggatcaga
     5101 tcagctggtt ggagatgctg gggacgatca gctctatggt gaagccggca atgatttcct
     5161 attcggagat tccccgttct tctccacaca agcaggatcg gatgtgctgg atggaggtga
     5221 gggcgacgat acgcttcagg gcggaggcgg cgaggatgat ctccagggag gtaggggaaa
     5281 cgaccttctg attggcggaa gcggactcga cacgtaccgc tttaacctgg gtgatggtgc
     5341 cgatacgatc caagatgatg gtgtgcaaag aaatcgactc atctttggcg caggcatcac
     5401 tgccgaatcg ctacgtttgg atgtcggacc agcagatacg ctggttctgc gcgtgggcaa
     5461 tgatgaagat gtgattcggg tcagcaattt tggactgaac gatcccgccg agtttcatac
     5521 tatccgacag tttgaatttg ctgacggcac agtgttgacg gatggcgagt tgctcgcccg
     5581 agggtttcag ctgtcctctc cggaaaccgg tgggacgcta caaggaacga gttttgacga
     5641 tcgtatgcaa ggcagccaag ttggggattt attgtatggg cgtggtggca atgatgtgtt
     5701 actcggtggt gtgggcgatg atgtcttgca aggtgatgaa ggtgatgatg aactagatgg
     5761 tggcgccgga aacgaccgac tctatgggaa ccaaggattc aacgtcttgc gcggtggcga
     5821 gggcaatgac attttggaat cggtgggttt gggagaccag ctgcttggtg gtggaggaga
     5881 tgatgcatat catctccgct ctatgggcca agtcatcacc gaggaagtga atgcaggccg
     5941 ggacacgatt tacctcactc cgaccgaatc gcttacattt caggcgccgg acaatttcga
     6001 gaatgtgagg atcgaggatg atgtgtacct cgattctacg gtccaggtga atttggttgg
     6061 gaatgctttg gacaatgagt tgtccggctc ccaccggttg gacggccaag caggcaatga
     6121 tacgttgatt gggaccggcg acaatacctt cgtgttcggc cctgggtacg ggcaggacgt
     6181 ggtgaggacg gggctacaag tgtatgccca ttcggggctc gatcaggtac aattcctcca
     6241 gggtgtgacc ccctccaatc tcagtctcga aagacaggca aacgatctcg tcgtgaaggt
     6301 taccggaact gccgacgagt tacgcgtaca gtcttattac gagtctccag cgaacatggt
     6361 agatcaattc ctcttcgcgg atggagccat ctggacctct ggagagattg aaaatcgcgt
     6421 acgaaccttc atcggcgtcg gggctgacga atcattctat ggttcagctg gtgacgatac
     6481 gatccgaggc atggggggga atgatcagat ccgagccaat gctgggaatg atgtgcttga
     6541 cggaggcgtc ggaaacgact tcctggaagg atatagcgga aacgacacct atgtgttcgg
     6601 ccgcggttat gggcacgata ctattgatga tcaaggtgac agcgctgata tcgataccct
     6661 tcaacttgtc gacggaatta caccgggcga tatcaccttg caggcaacgc ctgattttca
     6721 aaccgacgct cttctcacca tcaagaatag tgcggatcaa gtgacgctcg ctggattttt
     6781 ccagtccgat gggatgcgcg ttgatcgaat tcagtttagc gatgggacga tgtgggatta
     6841 cagcgccatg ttggcccata cggaaggcgt caatctggta ggcaccgaag acacggacta
     6901 tttttacgga aatgtgacga acgatacatt gttgggggta ggtggcgacg acgtgctctc
     6961 aggaggcgca ggcaatgaca cgcttgatgg aggcactggc gcagatcgca tgaccggtgg
     7021 ttccggaaat gatgtgttta tggtcgacaa tatcaacgac atcgtgatgg aatcagtcgg
     7081 tcaaggtatc gatacggtgt acagtagcct aacctatacg ctgggttcga acctggaaaa
     7141 tctgaccctc accggaagcg ctgccatcaa tggtactggc aattctctga ataatatttt
     7201 gactggaaat agtgcggcaa atgtcctgac tggtggggcc ggaaacgaca cctacgtggt
     7261 gggccttggc gacaccgttg tagaggcggc gagtgcgggt actgacactg tcaggtctga
     7321 tatcagttgg acgctgggca cgaatctcga aaatctcatc ttgaccggca ttgctgcggt
     7381 taacgggacc ggcaatagcc tcgccaacac cctgaccggt aacagtgcgg ccaatattct
     7441 gagtggcctg gctggggcag atacactcat cggggctcag ggggatgatc tttacattgt
     7501 ggacaacgtc ggcgacaaga tcactgaact agccggtgaa gggcttgata cggttcagag
     7561 ttctgtgacc tacacgttga gtgccaatgt ggaaaatctt acgctgagcg gcacctccgc
     7621 aatcaacggg accggcaacg gactggacaa cgtgataatt gggaatagtg ctgccaatca
     7681 gttagtcgga ggtgcgggaa atgacacgta tgtcatcggc tcgggtgata ccgtcgtcga
     7741 gggaataaat gccgggatcg atactgttca gagttccgtg actcatacgc tcgcagccaa
     7801 tgtcgaaaat ctcaccctca taggcaccgg tgcgatcaac ggtacaggta acgcgcttaa
     7861 taatattctc gtcggcaata atgccaccaa caccctgacc ggcggcgccg gggatgacac
     7921 gtatgtggta gggacgggcg acacagtggt cgaggctttg aacgctggcc ttgatacggt
     7981 gcaaagcacg gtgacctgga ccttaggtgc gaatcttgag aaccttactc ttagcggagc
     8041 agccgcgatc aacggaacag gcaatactct agctaattct ctgatcggta acgccggcaa
     8101 caacgttttg atgggtggcg acggcaatga tacgttgtcg ggcggaaaag gaaatgatgt
     8161 gctcaatggt gggacaggga acgacctgtt tcaggtggct cgcggagatg gccaagacac
     8221 gattacagat gccagcggga gcagcgatcg cttgagtttt gcgtcgggga tcaatccact
     8281 agatcttatg ttgagtcaga gcgctaatga tttgcgcatt gccgtctatg gttcgaccga
     8341 tcaggtgacc attaccaact ggtatgccgg tgcagccaac cagattgaaa cggtccaagc
     8401 aggcaatggt caacaattga tgagtactca ggtgaatcaa ctcattcagg ctatggcggg
     8461 atttacccaa cagactgggc tcagttggga tcaggccatt gctcagcgcc cacaggacgt
     8521 gcaacaaatt cttgccgcaa actggcatta aggccgagcc ttcgccccgc atacaaggcg
     8581 gggcggagga ttcttatcac cgttgtcttc ctcctcctga gattgttcag gcccgatccc
     8641 cacttatttc gttcgaatga gtgcctgtga cctggccgtg ccccgcgcag attcaccata
     8701 ccaaagaggt acagcctccg tgtaatggct gaaccgaagc gcgatggtac attcagaaca
     8761 tccctgcaag ccaagtacat cgcaatatca tcgcgccccc atctggcgag cgcgagttga
     8821 ggaggcttac gatgtcgaaa caccgagggt tctgcttcac ccttctcctg ctgctcgttg
     8881 gtgtggtcga aggatgcaca acgatgccat cgggcacctc cacggtccta cccagtagcg
     8941 catttttccc cctcgatgcc tcggagctca agccgcttca ggccattgct catgcgcagg
     9001 atgcgagaat gaagaattgc cataagggcc cagcctgtga agatgcctac tacacgcgag
     9061 cgttggtggc gttgtttgaa aatcgggcgg atgccattac ggtgtttcag gaattacgca
     9121 cggccatgcc gaacagccgg tatgaggttg cgacggtggg ctggctcaat ctgcttcagg
     9181 atgcatcgct atcctcagac cataacaagg cgcttatggt tcagttgaaa caagaagtcc
     9241 tccacaacct cttagaccgg tctgaagtgg ctacggcgag gccggtaaaa gatcacgacc
     9301 gccgtgttgc agagctcgcc cggtaactat cccgccactc cttcctcttt cccaccaggc
     9361 cggcaaggac cttcttgccg gccaacatgc cgccgccggt tgtatcgcct ttctccggga
     9421 ctcgcttttc ccaaccacta tctcaccttg ccgacagcga catcactttc aaccgtgtct
     9481 cggattttgc cgcagttgag tttggctgcc cgcacggcta cactttgccg ggcgtggtga
     9541 cctgcctctg gatctatcgc agatgggagg aatgtatgcg actcttcagt gtaacggccg
     9601 tgttgtcagc catcatgctt gttggcgtag ggatggcctc ggctgccgaa cctcaggccg
     9661 cggcaagcgg tacttctgcg gaaggtatca aaggcaaaga tggcgcgccc atggtgctga
     9721 ttccggccgg gccatttacg atgggcagta acgacgggct tcccgctgaa cgaccggaac
     9781 ataccgtgac tctcgacgcg ttttatatcg atcgctatga agtctccatg aagcactatc
     9841 ggtcgttcct cgtagaggcg aagcacgatg ctcctccgac ctgggatgat gaagccgcag
     9901 agacggttgg tgatcgtccg gccgtaggaa tgggctgggc tgatgcaaag gcctattgtg
     9961 cctgggcagg aaagcgtctt ccgacagagg ccgaatggga aaaggcggcg cgcggtaccg
    10021 acgggcggcg atacccgtgg ggacaaatgc agcccttcgt cgacattgcg aattacaatc
    10081 gcggggtatg ggtgagcgag gcggtgacgc ttacctcagt gagtggcggt gtcgagggga
    10141 tgagtgtacg ccatggcttg aaagaaggcg ggcgcagtcc ctatggcctt caccacatgg
    10201 cagggaatgc cgccgaatgg gtggcggatt ggtacgatcg ggaatactat tcgaagagtc
    10261 ctgaaaagaa tcccactggc ccggaaaccg gagagaagaa agtgattcgt ggagggtcct
    10321 ggtcagatct gccggtggcg ttacgatcat ctgcgcgagt ctcggcggaa ccggattttc
    10381 aggatcgagc gatcggcttt cgttgcgcca tgactccggg actatagcat cggctattat
    10441 tcacgcgttg ccgagactag tagtctttct tcatctgttg acgcagtagc gtgtttgttc
    10501 aatgtgcgtg taaggttcat ggtgccggtg tatggtcgct ccaaacgatt cttcctctgt
    10561 tccgccctca cagcataacc ataaagcgat tgcggcattg gtcgccggct tggtgttgtt
    10621 gctggctctg gtgggaattc aaaatgctcc gccaccgtcc gcagtggctc cggctgttcc
    10681 gaacatagac ccgaatatgg tgccgttggt gacgggggag gagcctattc aagagctctt
    10741 tgttcgcgcc ggttgtgtgg tttgccatcg gattccgggt atcgtggggg ctgatggaaa
    10801 ggtcggccct ccgctctggc tgggcaagac cggtgcctct cgcctggcag atccacaata
    10861 tcgggggcag gcgcaaacgg ttcgcgaata tattgttgag tcagtggtgt cgccaggcac
    10921 ctatgtggtg cctggttttc ctgcggacac gatgccggtc tggtatggga ggaaattgac
    10981 tgcaggagcg ctggataaga tcgccgcgta tctcgaactc gctacggatg agccgtagcc
    11041 tgggcttcat acacctggtt gcttgcgtga ggcctagagc agtagctcac tcctgtccgt
    11101 catcatcgtt tcttgccgcc cccttggctc atctgtccct ggatttggaa gcccttttca
    11161 tctctttcgt ttttgtcgag cagggctgcc actgcatcat caccttttaa tcccgccagt
    11221 ttaatcttga gccggagatt gttggcgcta tcagcgttga tcaaggcttg ttcaatgctg
    11281 attttcccgg ccttatagag ttgtaagagc acatggtcaa aggtctggca cccttcatca
    11341 ataccttgtt ccatcgcttc ttttaacgta tcaatttctg atcgcttgat cagatctttg
    11401 atgcgaggcg tatcgagcat gatttctaag gcaggtaccc gtcggccatc gagtgacggg
    11461 atgaggcgtt gcgagataat ggcccgcaga ttgagtgaaa gctggaggta aatctgggcg
    11521 tgccgctcca ccgggaaaaa gttcatgatg cgttcgatgg actgattcgc gttattcgag
    11581 tgcagcgtcc cgaggcagag gtggccggtt tccgcaaagg tgatggccgc ttccatggtt
    11641 tccgtgtcac gaatttcgcc gatgagaatc acatccgggg cctggcgcaa cgtgttcttc
    11701 agtgcattgc cgaacgaatg cgtgtcgaaa ccgacttccc gttgggtgac gatcgatttt
    11761 ttgtggtgat ggacgaactc aatcggatct tccacgctga taatgtgccc cgcatgcacg
    11821 gtgttcctgt gatcgatcat cgccgcgagt gaggtggatt taccggagcc ggtggcccct
    11881 acaacaagga ccagcccgcg tttcgtcatg gcgatatctt tgacgatggg cggcaggtcc
    11941 aactgctcaa ccgtcatgat ctccgccttg atgtgacgaa acactaaccc tacgtttccc
    12001 ttttgccgga agatgttcac gcggaaccgc ccgaggtctt tgtaatacag ggcgaggttc
    12061 atctccattt tctcttcgaa ttcgccacgc tgctggccgc gcatcagagc cagtgccaat
    12121 gactccaact gttcattggt aaacggtggc gcatctgttc gatgagtcga gccatggatg
    12181 cgataaatcg gtggcgcatc gacggtgaga tacaggtcgg acgattcctg tttgaccatc
    12241 acttcgagga gtgtgcgaac atccatgaga tgcctcttat gcggctatcg gtagacgaat
    12301 accgtgaatg gggcgttagg ccgcgccggc cgtgttaaaa aggttgggat tcatgctgcg
    12361 agactgggcc tcggctttgg tcaccatccc gcgcgtggcg aggtcgacca gggccatgtc
    12421 catggtctgc atgccgtctt tctggctcgc ctgcatgatg ccgggaattt gatgcagctt
    12481 accttcgcgg atcaggttac gcaccgcagt ggtgccgacc atgatttcca cggctgcaat
    12541 ccggccacca cttttctttt tgagcagcgt ctgtgttagc accgcttcca aggtttccga
    12601 cagctgcgcg cggacttgcg cttgctggtt cggaggaaac gcatcgatga tgcgatcgat
    12661 ggttttcggg gcgctagacg tgtgcagagt cgcaaagact aagtgcccgg tttcggcagc
    12721 ggtcaacgcc aactggatcg tatccaggtc ccgcatttcg ccgacgagga tgatatcggg
    12781 gtcctcgcgg agtgcggcac ggagtgcatt ggcgaatgac agggtgtgta cgcctaattc
    12841 gcgttggttc accaggcact ttttcgattt gtgcacaaac tccaccgggt cttcgatggt
    12901 gagaatgtgg ccttcaaagg tgttgttcag atagtcgatc ataccggcga gggtggtgga
    12961 tttcccggat ccggtggggc cggtgacaag aatcaggccc ttctcacggt cgcacagttg
    13021 tcggagaatg ggcggcatgc cgagtttttc gagcggaaga atttccgttg gaatggtccg
    13081 gaagacagca cctaacccgc ggccctggac aaagacgttc acgcggaatc gagcgatgtc
    13141 gccgaggtcg aaggaaaagt cacattcccg atgttcttca aagtttttgc gctgggcatc
    13201 gctcatcatg tcgtagatga gggcgtgagt ttcatcctgt gtcaggggtg gatggtcgag
    13261 ctttttgagg tcaccatgca gacggatcat cggcggttcg ccggcgctga tgtgacagtc
    13321 cgaggcacct tgctggacac caaaggtcaa cagtttggag atgtcaatca tcgggtcgct
    13381 ccattgaaag agaatgtgcg cggataaaac gaacgtagtt atgtctagaa gatcaaggct
    13441 cgctcgacca tttcgatcat gccataggag ggtgagaaat caagaaaata tccagaagag
    13501 gcgacgacga aatgcccgtt gctagggccc taactcttgt ggtcatcggg agatgactgg
    13561 ggagtcgtca ggtcagatca gattgatgga tcgaccggcc tgttctgcag caatcagtgc
    13621 gcgatcgatc tgctcatcgg tatggtccgc cgtgatggtg aagcgaattc tactggtagc
    13681 aggaggcacg gtcggaggac gaatggcggg ggcatacacg ccttgtgcaa ggagggcctg
    13741 cgctaacttc atcgcacgat cgggatcgcc gataatcacc ggcaggatcg gactctccga
    13801 tgccgcgagg cggaatccca gccgaaggag accttgtgcc agtcgttcgc ggttgcgcca
    13861 caggctggcg cgccgctgag gttcctgctc gatcacctgg attgcagcgg tcgccgcagc
    13921 ggcagtggcc ggaggcggag cggtggtgta aatgaacgcg cggcaggtgt tgaccaaata
    13981 ggcgatgaat gtggctgaac cggtcacgta tccccccgcg ctcccgagtg ctttgctcaa
    14041 ggtccccatg tgatagggca cacgcgattc gacctcacaa tgttcgaggg tgccgcgccc
    14101 ggtgcgtcca agaattcctg tgccatgcgc gtcgtcgaca tagagctctg caccataccg
    14161 ttccgccagc tgtacgagat ccttcatcgg ggcaatgtct ccgtccatac tgaatagccc
    14221 atcagtgacg attagtgtac gtcgaccggc gggacgacgg gcgagcagtt gctccagatg
    14281 attcatgtct cgatgatggt acacacgaaa ggtggctccg ctcagacggc acccgtcgat
    14341 gagactggcg tgacagagac ggtcggcaag gatcagctcg tcttttccga tcagggccgg
    14401 aatcacgctg atattggcca ggtacccggc ggcaaaggtg agggctgctt ctgtgccttt
    14461 gaatccggcg agggtgcgtt caagcgtctc atggggtgtg agcgtcccgc aaaccaggcg
    14521 tgccgcgcca gcgccggctc catatcggcg ggttgcctcg atggcagcat tcaccacagt
    14581 tggatgcgtg gccagcccca gataattgtt agaggagagc agaatgatgt cacggccgtc
    14641 caatgtgacc gttggtccgg tcgccgaggc gatcgtgcga agccggcgca agagatgttg
    14701 atcatccagt ttctggagat gtttttcgaa catgggtgcc cgggctttga cgaggccaaa
    14761 catttgacaa ggctgagtgg tccctagtat aaggcgcgag gctctgagaa acgaccgtct
    14821 tttccaaccg agaccattgt tcgaccatga gttatcgaat cagggttcct gcaaaagttg
    14881 acccgctcga cgaagcccac ctgttgaccg gcgttgatcg ggttcttcat gtattgcagg
    14941 aacagcggcg cgccgtcttg gcggggctgg gggtggtgtt ggttgccgga gcggtcgtcg
    15001 ccggtgtgat gtggtacgac tatcaatcca cattaaaagc gcgagagctt gatcaggaag
    15061 ccacgctgca ctatctcaat cggcccgcag acgacccgaa gaaaagccag gaacagctgt
    15121 cccaggctat cgcgctctat aagcaagtgg tggagcaata tccgagaagc cctgctgcgc
    15181 ccttggcctt gttccatttg gggaatgcgc aggttttggc gaatgaagtg gatgccggta
    15241 tcgaaaccta caaacggttc atgttgttgt acggttccaa cacctcgctc ttaggattgg
    15301 tccagcaacg gttggcctat gcctacctag ccaagggtga tcgcgatcag gcggtgaagg
    15361 ccttcacggg cattctcgac gtccccggag ccctcaacaa ggatcatgtg ttgttcgagt
    15421 tggcaaaaat tgaagagtcg cagtcccgtc cggagggagc cctcgcacac taccaggatc
    15481 tgatgaagaa ttatcccaac tcgcccttca ccagtgaagc tgcggtacgt gtgaaagtgt
    15541 tggaagtgaa gaaagctccg gaaaccccgg cggcagctcc cgcaccggcc ccagcagcca
    15601 gcgcccctgc ttctcagcct gaacagcctg ccaaggcttc aacgggcaag tcggctgcct
    15661 cgtcttccaa gaaaaagcag tagcctggtc tttcccctta cagcgggaga cgggtcgacc
    15721 tagttggcga tgaccaggcg gcttcctgcc ttaatggtgg gaccggtcag gttgttgttg
    15781 gacttgagcg ttttgactga gatgttaaac cgtttggcga ttttctccag cgagtcgccg
    15841 acgcggacgc gataccaatg tccagaatca gtctcgttct tgaaatgtcg gcctttgacc
    15901 tgaaccggca ttggcagcgg cggaaacttg tgagtcggag cccgttctaa taactgttcc
    15961 accttctctc tggtgcctac gggaaccttc aaatgatatt cggggtcatc cggaggcgtg
    16021 gcatcgcgcc gtaattccgg gttgagcacc ttcagttcct ggtaggaaat ccccgtgaca
    16081 ttggcgatgg ctttgaaatg gatggggcgc ctcacgacca cctcttcgaa ctggtgaggt
    16141 cggacatcgt tttgggggaa gccataccgg tccggattct tagcgatgat ggtcgcggcc
    16201 ataaaccgag gcacgtattc tttcgtttcc cgccggatga gccgggtttt cgcaatgtcc
    16261 gaaaagcttt ctgcttgggc cgtgtgcaag gcccgcatca ctttgccctc tcccgcattg
    16321 tacgcggcca tcgccagggg ccaggtgccg aataaatcgt acaggtcgcg caggtagcgc
    16381 gcagctgcca cggtggactt caccggatcc cggcgctcat ccacataact gtccactcgc
    16441 aacccataga ccttggccgt gcctttcatg aattgccagg ggccggcagc ccgggcacgt
    16501 gaataggcgt ggggattgaa accgctttct accaatgaca gatagatcag atcgctcggc
    16561 agctggaatt cagagaaaat gctatcaacc agcggtcggt aatggctgag tctgatcagc
    16621 cactgctcga atcggttgcg gatcgcgaca ttgaagaagc ggatatgacc ttgcacggaa
    16681 gggtcgagca cgatcgggac gttgtattcg gaaacattgt ccgattccga cccttgctcc
    16741 acggcggctt cctggatgac ttcagccgga ggggtcaaca aatcggcaaa tctcgcggtg
    16801 gtaccgctga gcggcttgag ttggagcagg gcttcggccg gaggaatctc cgtggacggt
    16861 tcgctcgcga ccgcagctgg gtcctcggcc gcctcacttt ccgcggcggc cgtgcgatct
    16921 tcttcctcta agagatagtc aggttcttgc gccgggggtt ggaggctatc tggtaacgga
    16981 actgctggaa cttcggatgt gagagcgggc tccgtttgca tgggaatcgg cgattccgcg
    17041 cctgcagaac caggcgaaga aacagagagc agacatgcga aaagaaggga aaggggcgga
    17101 acgccagcgc tggaaaaacg tgcatgcata aggtacctac tagacggttc agggaaggga
    17161 ggtgaccgac tctcgaatcg tagcctatcg aggaggtgag ttcttgtcaa gaaattgagg
    17221 gagttgagtt gctgggcgaa cgaaaccttt caggatctca gcatcaggtc cgccggaaca
    17281 ttttgaaagc aactctccgg taaaggcagt gaagtcgctg aaaagacggg catcttcctg
    17341 ggaacttacc ttgacacctc tggggggcta tggtagcgta cgccccttct aaatccctga
    17401 cattgttagt tccgtacagc gaggaggatc gtcccatgct caaacggtat ttgctggcac
    17461 ccggccccac ccctgttcca ccggaggtgc tactggccat ggccagaccg atgattcacc
    17521 accgggcacc cgagttcgat aaattgtttg ccgaggttcg tgaggacctg aaatggctgt
    17581 tccagacgcg caacgatgtt ttgatcctcg cggcttccgg caccggcggc atggaagggt
    17641 cggtttcaaa cttcctctct cctggcgaca aagccctgac gatcaacggc ggcaagttcg
    17701 gtgagcggtg gacgaagctc tgcaagacgt ttggcgccca ggtgacggag ttgaaggtgg
    17761 agtgggggcg cgccatcgac ccccaagccg ttgccgacgc gttgaagaaa gacccctcga
    17821 ttaaagccgt gtacgtgcag gcgagcgaaa cctctacggc cgtcgcacac gacgtcaaag
    17881 ccctggccga aattgtgaag caatacgaag acaccattct cgtggtggat gccattacgg
    17941 ccctgggagt gctcgatctg aagaccgatg cttggggact ggacgtcgtc atcaccggat
    18001 cgcagaaggc cttgatgctg cccccgggat tggcctttgc cagcgtgagt gagaaagcct
    18061 ggcgtctcgc cgacaaggcg aagaacgcgg ccttctattt caacttcaag cgggagcggg
    18121 agaatcagca gaaaagcacc accgcctata cgccggcggt ctccctcatc ttaggcctga
    18181 aggaagtgat gaacattttg aaggccgagg gcctggaagc cgtcttcgcc cgtcacgcga
    18241 tgttggcgac ggccatgcga gaaggcgtca aggcggcggg actttctatt ttcccccagg
    18301 agcggccaag cgatgcatta acggccattt cggccccgga aggtgtggac ggtcaggcgg
    18361 tctacaagaa cctgcgcacg cagtatggta tgacggccgc tggcggtcag gaccatctga
    18421 aaggcaagat cttccgtatc tcgcacatgg gatatatcga tagctttgac gtcatcacgg
    18481 ccttggcggc tgtggaaatg gtcatcaaag ggttggggta tcccgtgaag cttgggagcg
    18541 gggttgccaa ggctcaagaa attattatgg gaaagtccta gactcacctt caggtaaggc
    18601 gccacgacaa tgaaaatctt ggtcagcgac agtctttcga agcagggtgt agaggtgttg
    18661 gagaaggccg gctttacggt cgtggtgaaa accaagctgc ccaaagagga gctgctgaag
    18721 gaaatcaagg atgcggacgg gttgatcgtt cgctccggga cgaaagtgac agcagaactc
    18781 atcgccgcgg cgagccgctt gaaggtggtt ggacgagccg gttccggtct ggataatgtc
    18841 gatacccccg ccgcaacccg tcgcggcatt gtggtcatga acacgccggg tggcaacact
    18901 gtgaccaccg ccgaacatac gatggccatg attttttcta tgtcgcgccg tattccgcaa
    18961 gcgaccgctt cgaccaaggg cgggaagtgg gaaaaagaaa agttcatggg cgtggagttg
    19021 tataacaagg tactggggat cgtcggcgtc ggacaaatcg gcggatacct taccaagctc
    19081 gcccagggcg tcggcatgca ggtgatggcc tatgacccgt atctcgcacc cgaacgggcg
    19141 cagaaaatgg gcgtatccat cgtggaattg gacgaattgt tccgccgggc cgatgtcatc
    19201 tcggtccaca ctccactgac ccctgaaacc aagtcgctca tcaacgcaca ggctatcgcg
    19261 aagatgaaaa ccggtgtcat gatcgccaac tgcgcccgtg gcggcatcgt ccacgaaggg
    19321 gatctctgcg aagcattgaa atcgaagaag gtggcggcag cggcgttcga cgtgttcgaa
    19381 gacgagccgg tgaaatcgga caatcctctc ctggctctgg acaatttcat ttgctccccg
    19441 catatcggtg cgtctacgac tgaagcgcaa gagaatgtcg cggtgggaat cgccgagcag
    19501 attgtcgaat actttaccaa agggatcgcc cgtggcgccg tcaacattcc ctctgtgtct
    19561 cccgagctct tgccgcagct ccagccctat ctctcattgg gagagcgtgt aggtttgttg
    19621 caagctcaat tattggaagg cggacttgag cggttgaccg tcgaatatag tggtgaggtg
    19681 gccggactga atgtcgcccc gttgacgatt gcggtcttga aagggctcct gacgcctatc
    19741 cttgaagacc cggtcaacta tgtgaacgcg ccggtcgtcg ccaaagagcg cgggatcgaa
    19801 gtgaaagagg tcaagagcaa cgatgccggc gatttcacga gcgtgattcg cgtgcgggtg
    19861 gaagcgggga aaaagtctca tcaagtggcg ggaacgctgt atcaccgcaa agatccgcgt
    19921 atcatcgaaa ttgacgaatt caaggtcgaa gtggtcccgg acaaccattt gttattggtc
    19981 ctcaatgagg atcgtccggg agtgatcggg acggtcggac atattcttgg cgaccacaac
    20041 atcaatatcg cgcgtatgca gtgctcacgt gaggagcgag gcggcaaagc cctcttgatt
    20101 ttcgggctgg atgcgccgct gcctgcgccg gtgctcgatc aaattacgaa cagcaagcac
    20161 atcctttccg taaaggtcgc cgacctgtcg aaaggtctgt agattgcccg gcagtcgatc
    20221 atgggctccc tttcgttgaa ggcctctgct tcctccacgt cgctggtggc gggtcgggaa
    20281 cggtcgctta ttccggtcgg aatgagcacc atccttcccg ccgcggctcg acgtattcgc
    20341 caccttgagc agactctgct ggccgttctg gcgcgaggcg ggtacgacga aatcattctg
    20401 ccgatgttcg agtatctgga cgtcctcgcg ccagggcttg aaccggaact cctcgaaaag
    20461 tgttatcagc tggtcgatcg aacgacgggc cgcctgatgg tgttgcgccc ggatgcgacg
    20521 gcacagatcg ccagaaccgt cgccatgggt atgatgggcg aacggctgcc gttgcgcttg
    20581 tgttatcgca cctcggtctt tcgctacgaa cgcgagcatg ccggccgcga ccgggaaatt
    20641 tttcaagtcg gcgcggagct catcggggtt gacggggtga ccggggatgc cgaggtgctg
    20701 acacttctgc tggactgttt gagtcaggtg ggactcagcg cctttaaagt ggcggtcggg
    20761 catgtcggct tctttaccgc tcttcttgtc aggtccggtc tctcaagcga aggccagaaa
    20821 cgcgtggagc aagcggctgc tcgcaaagac atcccgctcc tcgaggaatt gctggcgcgc
    20881 gagggactgg cccgatccac tggacggatc attctggagg tcctggaact atgcggaggt
    20941 cccgaagtcc tgtcgcgcgg acgtaagctg gttgggcgcg ataaagtgct attgaaaccg
    21001 ctcgatcgac tggcgcaggt ctatgaacgg ttggtttcgc cgggacagga atcagtcctg
    21061 atcgatctgg gagagtttcg cggctttgaa tattatgacg ggatagtttt tgatgtcttt
    21121 gctccgggga taggcgcaga attgggcggt ggcggccgct atgatcactt gatgggccgc
    21181 tttggtcgca cggcggcatc ctcgggattt gctctggatg tcgatcgact attccgagct
    21241 atcgattcgt cggtccgccc gcttccgccc gatgcgacga gatctcaatc gaagcgtcgg
    21301 cctgcgacgc ctcctccgtc acgacggcca aggagccgag catgaaatcg ctctcggccg
    21361 ttgacctgac agcgcctcga ctcccgccac agaacatcga agccgaacag tccgtgctgg
    21421 gcggaattct attggacaat acggccatgg ccaaggcgat ggaagtcctg acggatcagg
    21481 agttctatcg gactgctcat cgcaagattt atcaggccat gctggaactg tccgatcgtg
    21541 gcgaagtgat tgaccagatc acgttgacgg aatgcctgaa gggacgttcg gaattagatg
    21601 cggtcggtgg atcagcctac cttgccgagc tggtgcaggc cgtccctacg gccgcgaata
    21661 ttcggtacca cagcaagatc gtccgggata aagcgctctt acgagggctg atcgaaacct
    21721 ctacggaagt cattacccgc ggatacgacg gtacagcggc ggtcgatgag ttgctcgatt
    21781 ttgccgaacg ctcggtgttc gggctcgccc agggcaagct cgatcgctcc ttcacccagg
    21841 tcaaccacat catcaaggaa agccttgatg tcgtggataa gttgtccaag cggaaagagc
    21901 gggtgaccgg ggtgccgacc ggctatattg atttggatga tctgacggcc gggttgcagc
    21961 cgtcagactt gatcatcgtg gccggacggc caagcatggg aaagacgagt ttggccctcg
    22021 gcatggccca acatgccgcc ttgcatgccg gaacggtcgt aggaattttc agcctcgaaa
    22081 tgtccaagcc gcaattggtt cttcgtatgc tgagttccga ggctcgtgtt gattcgcacg
    22141 cgcttcgcac cggaagactg caaaaggagg attggtggcg attggccgag gcggccggca
    22201 agctggagca ggccccgatc tttatcgatg actccggagc ggtgaccgtg cagcagatgc
    22261 gcggaaaggc ccgacggctc aaggccgaga agggccttga tttgctcatc gtggactatc
    22321 tgcagctcat gcagggcaag agcgattccg agtcgcggca gcaggaaatt tccgacatct
    22381 cccgctcctt aaagagtctt gccaaagagc tgaacgttcc cgtcattgcc ttatcgcagt
    22441 tgagccgggc cgtcgaggct agaaagccgc cggtgccgat gttggccgat ttgcgtgaaa
    22501 gcggcgccat cgagcaggac gctgacgtgg tgatgtttat ctatcgggaa gaagtctatg
    22561 agccgaacac tgagcggaag ggcattgctg agattctcgt cagcaaacat cgcaatggtc
    22621 cgatcggaaa acgcgatttg ttcttccacg accgattcgc caagtttgag aatctagaga
    22681 cgcgtgagag cgtttgacgc gttctttcac gggccggtat aattccgcat cgtttcctgt
    22741 accccgaaca ttccccctgc agattgagcg gatgaattgt gcaacgattg agtgaagagc
    22801 gtcggcagtc ctgcaccctc tgtaaacccg tgggaccaaa ggctgcagct gtcatgctga
    22861 tcgcgcttgc ttgtgtgacc gctgcgtgcc agaccgccgc gccgtcacgc tccacacaac
    22921 cggtcgtccc tgccgcggct cctccggtct ctcgcccctt gtctcccgca tctgattcgc
    22981 gtgcctccta tcacttcctg ctggggtatc aggccgagtt ggagcaggag acagaacagg
    23041 ctatcaaaga gtatcagctg gcactgcaga cagacccctc ttctcactat ctcaaggctc
    23101 gattagcggt gctctacttc accgcaggtg atgtgccggc tgcggtgcga tttgccgatg
    23161 aagttgcgga tgcgccgggg ctagatgcgc agatgctcgg gcaaatcggc gggatgtatg
    23221 cggcggcagg aaagccggac aaggccttgc ggctgttcaa tcgtgccatc gagcaggaac
    23281 ctcaacgcag cgaacatttt tttgccaaag ggctgttgtt ggccaatcaa aagcaatacg
    23341 ccgaagccga agaaactatc cgctccggca tcaaggtcag cccggactcg gcagtggggt
    23401 attactactt gggcagaatc ggtgtggagg cccgggattt cgataaggct acgacgcatt
    23461 tcgagcaggc cgtgacgttg aatccagcgt ttgaaccggc ctatgtcgca ttggggtctg
    23521 tgtatgaagc caagcaggat cgcgggaagg ccatcgatat ctaccggcgt tacttgcaag
    23581 gcgtgaatcc aaaaaatcgt gaaatccggc atcacctcat ccggctgcag gtgtcggcga
    23641 agcagtacga cgaagcgctg cgagagttac aggaaatgct ggaggaggat ccgtcagatc
    23701 tcgatgctca gttgcgaatg ggccttgtgt acggggaaca gaagaattat ccaaaagcga
    23761 ttcaacagtt gacgcagatc cttaccgtgc gcccctccga gctcaaggtg cgagattatc
    23821 tcgggtatct ctacgaggaa acgaaggatt acgcccacgc gattgaggcg taccgacaca
    23881 atttgacctt ggaaccctcg tatttcgagg ggcatctgca cttgggggtc ttgctgtatc
    23941 ggaccaagca gtatcccgag gccatccagc atctgaagga agccagccgc ttaaacccca
    24001 agcagcctga ggcgtatatc gttctcgggt tgtcgcattt tcaagtcgaa caatatgagc
    24061 cttccctcca agcctttctg gaaggaatcc ggcacaatcc agataatgcc gacctgcatt
    24121 tcaacgccgg cactgcctat gacaaattga atcgttttga cgatgtggtg aagtcgatgg
    24181 agaccacgct cacgttggat cctcaccacg ctgatgcgat gaattacctc gggtacagtt
    24241 atgcggaacg aggagtcaaa atcgaagaag ccatcgcact gaccaagcag gccgtcgcgc
    24301 tacggcctac gaatggatat tacgtcgata gcctcgcctg ggcctttttc aagaaggggt
    24361 tgctgagcga ggctctggcc gaaatgaagc gggcggtggc gctcgtcggc gatgatccgg
    24421 tcatctacga acatttgggc gaaatccatt tgaagcagca acatctctcc gatggtcgcg
    24481 aggctctcct gcacgcgctc gaacttgatc cctcgaatga caagctcatg cagcggtttc
    24541 gtgagctcgg tttgggcgat cctgccaaag aagagcgaat ccgccaagcc atccggcgtg
    24601 taacagagcg gaagacggct ccctcggctc agtaattctt gtctcacgct acagttgcct
    24661 cgtgccctct ataccgagag ggcatcgcca ccgattgttc atcacctcat tgccaatccc
    24721 gttcgtttcc acatagtcct ccagcggctt ccgcagcagt tcttcctccc cagccaacga
    24781 gctctacctg ttcgattcag tcaatttttg gcactttgcc tagctcggct cctcttcctg
    24841 tttgagcgaa cagggccaga attgtgaaat ctagacaaaa atgcctcggt ttgtcggcag
    24901 gttatgcggg aacagttaaa aggctcctct cctggcatgg ggcctgctta tacgtggcct
    24961 gtcggcagta cggacgggcg gagcatcagg atgggtttcc agagcgaaag tcttcgagcc
    25021 caatggtgac tgaatcaaag caagcagggt tcacactgat cgagctcatg gtcgtagtgg
    25081 tgattgtggg gatcttggct gcattcgcca ttccgaactt tcttcgatac cgcgcacaag
    25141 cgatgcaagc tgaggcacgg acgaacctgg cgggaatttt tgtcggtgaa atgtccttct
    25201 tcacggaacg gaaagagttc ggaaacttca cggatatcgg cttcgcggtg gccggaccag
    25261 gaacgaatcg gtatacgtat cgaaccgggc ttggccttgg ggctggattg ggaccgaacg
    25321 gaggcaatct ttgcggatct gccggcaact gcgacacgat tcaatctgaa gtgccggctg
    25381 ctggcgcgat cagtttcact ggaagcgtag gaatcgccac cacctcttcg gcaggtttca
    25441 ccgcaaccgc cgcagcggat cttgatggag attcgacgca tgacgggtgg tacgtcaacg
    25501 attcgaagca agggctgagt gccgctgacc cgaatgatgt atcgagttag gtgcgggtgc
    25561 caaaacttgt cacctgatga cggaaaaagg aatctcgttc aggcagccgt gaggagttca
    25621 aaagcgatcc taaatctaag tgattggaag atatgcaaaa agatacgcaa ctacaggaag
    25681 gaggtgttgg gcatagaacc tgctattccc aagatctgtc aggcgtgaac acgcggttcc
    25741 gaatgaagtg acaggaatca agaacgtgtc ggatgaagtg gcaacaatag gaaagggagg
    25801 cattatgttg aagcaagtga agggtcagaa aggttttacg ttgattgagt tgatgatcgt
    25861 cgtggcgatt atcggtatct tggcggccat cgccattccg aacttcttgc gctaccaagc
    25921 caagtcgcgc cagtcggaag ccaagacgaa cctgggcgcg atcttcgtgg ctgagacagc
    25981 ctaccttagc gaaaactcgc gctatggcag cttctcggaa atcggctatg cccttgccgg
    26041 tacgaccaac cggtacacgt accgcagccc tgccatcttg ggtaacggtg catcgtcaaa
    26101 caccgtgggc gtcgatcaga ttccttgcgg cgcgccggtc ggttgcaccg ttcagagtga
    26161 tgcttctcct gctgtgccgt ccctcgctag ctcgaccgct ggttcagtcg gcttcacggc
    26221 gtccgcggtg gccagcattg acaatgaccc gaccatcgat ggttggtcag tcaacgatat
    26281 caagggtggt ctgacccagg ctgttcctga cgacgtgatc agctaacgat cacgcgcagg
    26341 tctgctccga tgcgggggag gaacaatgtt cctcccccgt gttcttttgg ctgaccagat
    26401 ccttcccatt ctctgaacag cctgtttctt tcctgccctg tttctctcgc cgcagtattc
    26461 aatttcactt tcactttgcc aaattgccgg tctctgataa agcaggaggt atcggtcgca
    26521 tcaagtctgc gaacacacct atgcaattcc tgtcttcgat gccagtcatc gtaatgtgcg
    26581 cgctgatgat tttttcgccg cttcaggagg gcggaacgac gcatccagcg caaatgatca
    26641 tacggcttgt gattctggcc tggcttggtg gaacgctcat tcaggcaatc cgggcaggtc
    26701 gtttaattgt tccaaggctt ggagccagat atgtcgtcct ggcgttcctg ggctttgctg
    26761 tcgcagccac gattttttcc ccctatgtcc atcaaagtcg gcagtggcta gtgatgatca
    26821 cgggatacgc catcttgttt tatctgctgg tctctgttgt cgaccggtgg gagcgtgtgc
    26881 atacgttgac gatgatggtc atcgtgatgg gattaggaga agctgcctgg actatcgtgc
    26941 agggtgttgt gtggaatgtc gtcagaccca gcggaacatt tttcaacccg aatttcctcg
    27001 ccggctatct gaccgtcagc tgggcagttc tattgagcat cgccgtctac ggataccgga
    27061 aaagcgcaat attgtatcgg tcctcggtgt cgcctgtatt ctggtgggcc ggcatgggat
    27121 cagcgttggg cattgtcctg atcgcaatga tcttcactca gtcacgcgga ggtctggtgg
    27181 cctggctcgc ggccacagtg tggatcctga cggcccgata tggatggaag ttggcaggta
    27241 tgggcactgc cgcgcttctg ctcgcggcgc ttatcgtccc cactccgctc aaagaacggg
    27301 tgcttgctga gcatgaacag aaccctgttt cgtatgcccg ttggcagatg tggcaaggcg
    27361 caatagccca gatggccgat catccattgg gaatcggctt agggctctac caatatacgt
    27421 atcctttgta tgcgtttccg gtcgaagggg aaattacccg atacggaaaa gtcgcccaca
    27481 ctcctcacaa tgactatctt caaataggag tggagatggg cgtaggcgcg ctgctggtct
    27541 ttctggccgc cataggcctg gtcatgcggg accttctgca ggtgctccaa gcaagattgg
    27601 tacggtggca gagaagtctc atcctcggta taggaggcgg aggaatcgcc cttctcgtcc
    27661 atgccgcgct ggactccagt ttgcgtgaat cagccctcgc gatcctgctc gttctctgtg
    27721 cgggactgat cgtatcagtt gctcggataa ccagtggcaa cacagatgcc ctgcatgcca
    27781 tcccgattca gtcgcgcccc ttgtggggaa tgggtgccgc ctgtttgcta ctgttggtcg
    27841 gagcggaagt cagtcgatta gggatggcct ggctgacatt cgataaggca tctcggttgg
    27901 ccgtggcagg agacacagag atcgccatcc aaggattcaa gaacgcgatt gcgttggatt
    27961 caggcaaatc gctctatcac catggtctcg gatcggtcta tgcgagagac ttcgaagcgt
    28021 ctcgcgataa gcagctgttc cagcttgcgc atgcggaatt caaagcggcg attgagttaa
    28081 atccgttgga cagccgattg ttggcacttc tcgcgcagct ctatgtgtcg gcggcgcaga
    28141 cgccatcttt atcaggccgc ctagacgaac agcagatatt ttggttgcgt gcggcgttgc
    28201 gggtgtatga acgggccgtc cggctcgcgc cattttctgc tccctatcgg tatgaacagg
    28261 cgcgtcttca ttggatgctc ggcgagcggc cgttagccga gcagcaggcg aaggaagccg
    28321 agcaattgga gccgaatttt cttcctgctc gcgcgttgct ggctcgtttg tcggtcgatg
    28381 cgggccgcat cgatgacgcg aggcgggagt tgcaggaaat acaggtgcgg caggcgcgat
    28441 atgaacagtg gaacaaaaat aatatcgaca ggacattttt aagtgtcgat gtcgcgccgt
    28501 tgcgtgcagc cgttgaagca aaaggccttg ccgggtgaag ccaatgagag gtcgtacgga
    28561 catcaggtcg cgcctcagtc atgtgttgtg tgtattggtt ttgttgatcg gcacatcggg
    28621 agtgctccat attctggagg gagagcggac cgctgtcgca agggccgagc aattggcgta
    28681 tctcccgaag ggagaatatt tcaaactggc cgttctcggg tatcggcagg tcgtcgcaga
    28741 cctgatctgg ctacaggctg tccaacatat cggcgcgaag cgcgacacac agttgggcca
    28801 tacctggacc tatcatgccg tagatgtcct gacggacctc gatcccacct tcgttcctcc
    28861 ctatcaagct acgggcctct tccttggtgt gttggtcggc cgccatgacg aaggtgtggc
    28921 tatcttacgc aaaggtatcc ggcacaatcc ggccaattgg caactaccgt ttctcgccgg
    28981 ctatatctcc tactacgaac ggtgcgacgc ctcagccgcc ggagagtttt ttcagctggc
    29041 agcgcacgtg cccggttctc cagcgtacct tcctcaatta gccgcacgca tgactgtgga
    29101 aagtggagat cccactgcgg cgttagaatt tttagaccgt ttctcccgga gcgtagccga
    29161 tgatcgggtt cgcgaagcgc tgtttcggcg aatgaaagag attgttcaag agcaggacct
    29221 ccggtttctt gaagaaagca tcagacggta tcggctgaag tatggccagg cgcccgcgaa
    29281 ggtggaagat ctgatgctgc acggcatcat tccgcgactt cccgaggatc ctttaggcgg
    29341 gcaatatgaa attgatggcc tgactggcat cgtcagtgcg tcctcaaaac gggaccgact
    29401 acgtattcat gaaaaagtag cctgccgctt aggagttgcg tcgacgccct cgacacaggc
    29461 cgatgcaata gagacgctgc agcccgctgt gcagcaatga cggaggagtg ccgtggacga
    29521 tatcgtcaca cataatctga ccaaaaccta cgcgtcgggc tggcccgggc gacctccatt
    29581 tgtggctctg gatgggctat cgctcaccgt cggccgtgga gaaatttttg gatttctcgg
    29641 cccgaacggg gctggaaaaa cgaccacact gaaaattttg atgggactgg tgcgccccac
    29701 gagtggaacg gcactgctgc tgggacaacc ggcgggggat gtcgcaacgc gccgccgaat
    29761 cggatttttg ccggagtcgc cttactttta cgactatctc actgccgaag agtttctcgg
    29821 attctacggg cggttggccg gcctgaatcg ttcggcgatc acgcagcgag tgaccgatct
    29881 tcttgaactg gtcggacttg tggaagcgcg caccagacag ttgcggaagt tttccaaggg
    29941 aatgctgcaa cgcatcgggc ttgcgcaagc cctcattcac gacccggaat tgattatcct
    30001 cgatgagccc atgacaggat tggatccggt cggacgaaaa caggtacgcg atctgatttt
    30061 gagtctgcgc gattgcggca aaacggtgtt tttcagcaca cacattctgc acgatgttga
    30121 gatgatttgt gaccgtgtcg gcattgtgat gaagggccga ctggtggcca gcggacgggt
    30181 tgatgaattg gtgcgccagg atcatacacg atcggtcgaa gtggtctgcc aacagctcaa
    30241 ggtcgagggg aatgcgttca ttcactccct ggccacccgc gtgctgcagc agggacagca
    30301 atgtctgatt gttttgccca gtcctgatgc ggtcgatgcg cttgtgggcg agattcggcg
    30361 ccagggtgga cagttgctgt ccatcactcc gcacaaagcg tcgttggagg atttattttt
    30421 tcaagaggcc gcgcaggcga ccacgcgagt gttgccgcat atgaccagtg ggagggcgtc
    30481 ctgacatggg ggcgatcggc gttattgctg tcaatgcgtt ccgtgaaagt ttgcgggaca
    30541 agattttata caatctcgtg ctgttcgccg ggttgctcat cgggctatca gtgctgctgg
    30601 cggatctctc cattaccgag caccataagg tcattgcgga tatgggcctg gcggcgatca
    30661 atttgattgg cgtgatcatc gctattttcg tcggcatcag cctggtcaat aaagaaatcg
    30721 agcggcggac gatttatacg atcatggcga ggcccatcag ccggaccttt ttcatcatgg
    30781 ggaagtatct cgggttggca ctgacgctct tcgtgaatct ggccatcatg ctggcggtgt
    30841 ttctgcttac gctctggctc tatcatgtgc cgattcagac gtctcttttc caagctgtcg
    30901 cgctgatctt tgtggaaatc ctggtggtga cggctatcgc gctgttcttt tcgacgttca
    30961 cctccaccac attgagcgcc atttttacgc tgggtctcta tgtcatcggc catctcacgg
    31021 ctgacctgcg atcgatggtg gccaatagtg aaagcggtgc ggtgaaggga gtagtggacc
    31081 tgttgtacta cctctgtcca aatctggaaa tgctcaacat caaagggcaa gcggcggtgg
    31141 gtcttgtcgt ggctccggag tatctcatgc tcgcgtcact gtatggcctg ttgtatgcca
    31201 gtctcttgct gacgggggcc tgcctggtgt ttcagcagcg ggatttttaa gtcatcgcga
    31261 gagaggcgtg gttcatcgta gccatggagc ctgtcggcta ccggctatgt gcgtgtcgcc
    31321 cagagtcttg gaaatttgtt gccgcgctct tactgaaggg taagaaatgg tcttccagcg
    31381 ttacggcctc tcccccttga tcgtcctcac gagcatgtcc gcgcactgga atctgatccg
    31441 tcaattgacg aagcgggaga ttgccagccg gtatcgagga tcactcctgg gaatgttgtg
    31501 ggcattcctg caccccatgg tcatgctcac ggtctatacc cttgtctttc gaggcgcttt
    31561 tggaatgcgg tggggacagg acggggagag cgcgctcgat tttggactgc tgctgttctc
    31621 aggcctgatc gtccatgcat tgtttgcgga aagtattcat cgggcgccct acttgatcgt
    31681 caatcacagc aattacgtca aaaaaatcgt cttccccctg gaaatcctgg cgtggacatc
    31741 tctcggttcg gcgttgttcc atgcgttgat cagcgcactt gtgctgatcg cgttttatgg
    31801 gtttctgcat catgacctgc actggaccat gttgctggtg ccgcttctcc tgctgccgct
    31861 gtctctggtg accatcggta tctcgtggtt tctagcatcc gcgggagtct ttgtgcggga
    31921 cattggtcag gcgagtggtc cgttgacgac cgttatgctg tttctgtcgc cggtgtttta
    31981 tcccgctggg gcgtttcctg aggcctaccg gatcttgctg tatgcgaatc cgctgacatt
    32041 tctcatcacg caagctcagg atgtgctcat ttggggcagg ccgccatcct ggatcggtat
    32101 cggtctctat tgtgcgggaa gttatctgat cgcctggtgc gggctcctct ggtttcaaaa
    32161 gacacggaag gcgtttgccg atgtcctctg agtgggctat ccgtgtccgc caactttcta
    32221 agacgtaccg cgtgtacgat cgcccgcgcg atcgtggaaa acaatggatc cttccaccat
    32281 tgcagaaatg ctttgggcag tctccttcac gatactatcg cgaagtgcat gccattgagg
    32341 atgtgtcctt cgaggtgcag aagggggaaa cgcttgggat tatcggacgg aacggctcgg
    32401 gcaagtcgac gcttctccaa ttgatcgtcg gcacgttgag tccgacgagc ggcacgatcg
    32461 agaccgatgg acgcatcgcc gcgttattgg aattaggggc tggatttcat cctgagttta
    32521 cggggcgcga gaacgtcttt ctgaatgcca ccttgctggg tttgtcccag gcagacatcg
    32581 atgcccggtt ggatgacatg ctggcgttcg ccgacatcgg gtcatcgatc gatcagccgg
    32641 tcaaaacgta ttcgagtgga atggttgtcc gcctggcatt tgccgtgatg gcacatgtca
    32701 gcgccgacat cctggtgatt gatgaagcgt tggctgttgg cgatgcattt tttgttcaaa
    32761 agtgtatccg ctttcttaga gcgtttatgg aacgggggac ggtgttgttc gtcagtcacg
    32821 attcagcagc cgtcctaagc ctctgtcgca cggccttgtg gctggagcac ggacggatcg
    32881 cgggcaaggg ggaagcaaag gtgatctgtg atggatacct gcatcagcct acccctatgg
    32941 aaataccgcg ggagaaggtg gcggaaggtc ttccgtcatc agacccctcg gatccggctc
    33001 agcctataca gaatgactcg tcgaacttgg cggtattacc acatcatgag acgagatcgg
    33061 catcgcatag ccggccagca tggatcgaaa ccatcacctt gtgcaatgcc aacggtcgca
    33121 cggttcacac cgtcacgcag cgtgagcagg tgacgctgcg tgtctgttgc gtcacgtccg
    33181 tcggattggc aagtccgatc gttgggttcc tggtgcgcaa ccgcttcggg cagtctctgt
    33241 tcggcgcgaa tacgtgtgcc gcgggaaatg ctgccggtca cggtgttagt ccgggtacgc
    33301 gcttcgtggc tgaactggag tttgtcatgc cggtgctgca tccgggcgac tatgccatga
    33361 gcatcgcgct tgccgacgga acgcaggagg agcatgtcca gcaacattgg attcatgatg
    33421 cgttggcttt cacgtctgct ccttccgaat ggtgttttgg tctgatcgcc gtagaagtaa
    33481 tgcgcacaca gatctgcgaa atcgaagcac aggatgctca acatgaacgc ctccatgatt
    33541 ccattgcctt gtaaggtgtg tggcggcagc accttttctc atcatgacgt gttatggggt
    33601 gacctcattg cccagtgggg cctctcatcg cgtgaggtag actacatcaa cgtccagcag
    33661 ggaaccgcgt gcaccggctg tggctcgaat gtgcgctcca tcgccctggc cgatgcctta
    33721 ttgcgatgga cccgcgcgac gaccacattg caagagtggg tccatactcc ggtggctcgc
    33781 cgatgcgatg tgttggagat caatgaagcc ggatcgttga ccggcacgct gtcgacgctt
    33841 ccagggcatc gcctggtgcg gtatcccgag tacgacatga tggctttacc gttcgaagac
    33901 cgttcattcg acgtcgtgct ccattccgat accttggaac atgtgccgga cctgatgaaa
    33961 ggattgcggg agtgccgccg ggtccttcgt ccaggaggag cctgtctctt tacggtacct
    34021 gtggtggtgg gaaggctcac gcggggaaga aaagggttgc cgctgagtgt gcacggaagc
    34081 gaagggtgca tcgacaccgc cttgactgta catacagaat tcggcgcaga cgtgtggtgt
    34141 caggtcttgg agagcggctt tgatgaatgt cggatcgtgc cctatcggta tcccgcggga
    34201 attgcgctca ttgcagaggt tgccgaatcc agaccggtaa cagggggtca gtggtgaaca
    34261 agacagtaaa tgaatcaact gaactcgaag cgaccggaga gcgatttctt cctgagatgc
    34321 agggaaccat cgcactggag catttgcatc gctatgcctt ggcgaaggat tgcgctgccg
    34381 gaaaaattgt gctcgatatc gcctgcggcg aagggtacgg ctctgccgtg ctcgccggca
    34441 ctgccgaccg cgtctacgga gtggacattg cgccggatgc gattgcccat gccagagcta
    34501 tctaccagcg acccaatctc gagtacctgg tcggtgaatg cgcgaatatt ccccttccgg
    34561 actgtgcggt cgatctggtg gtgagtttcg agacgatcga acaccacaca gagcatgaag
    34621 ccatgtttgc cgaaatcaaa cgtgttttgc gaccgggcgg ggttatggtg atgtccagtc
    34681 cggataagct ggaatacacg gaacgctccg gtcacgccaa tgcctaccat gtgaaagagc
    34741 tctatgcgga agaattccag cgactcgttg agcgctactt caatcgtgtg gccatgctcg
    34801 gccagcgggt gctgtatgcc tcggcaattc taccgcaagg ggcgccgggg gaagcggttc
    34861 attactcccg cgacaaagat tcggctattc aggctgcgag cggattggtg cggccctgtt
    34921 accaagtggc gatcgcctcg gatgcagcct tgcccccagt gttgtccagc ttgtttgaac
    34981 aggccgggga gtcagatcct gaaattcatg cgctccaaca tcagcttgag catgccggtc
    35041 gcagaattct aggactggaa gaagaggtgg cagggctcaa ggcggccatc gaactgatga
    35101 tgaattcgca ttcctggaaa gcgactagac cgcttcgcgc cgtggcgaat gcgctacgag
    35161 ccgtcagcgg gaaccgagtc gagaagagcc gccgcgcatg aaaacatgca cacattcaga
    35221 ctgcgcgatc ccgttatcgc gcggtgggcc aggcatagta actggcgtct tgccgaccgc
    35281 cttgttgtga gccatgaatc gtctcatgca gcgattgatc aaagccattc ggttttcacg
    35341 cttcgccatc gtcgaaggtc tccggtggta taagcgtcat ggtcgactgc ccaggaaagc
    35401 cgaatgtgcc gatctattcc ggaaagcttt ctttcgattc aaaatcctcg cgcaacgaat
    35461 ctcgccggct cctaaaacca tcgagccagt actccctccc tatgacgcct ggctgaaggt
    35521 caatgtctgg aaccggcgaa tgcgcgacga tttactcgac cgattgagcc ggcatgccgg
    35581 ccgtttgccg acgatttcgc tggttatgcc ggtgcataac cccccacttg agtgtctcac
    35641 caaagccatc gattcagtac gggcgcaggt gtgcggagaa tgggaactct gcattgccga
    35701 cgactgcagt acgaatcctg ccatacgaga tgaattggag cggttccgcg cggaagaccc
    35761 tcgtatccgt ctcacctacc tggagcggaa cctcaatatc agtctagcca caaatcgcgc
    35821 cgcagatctg gcgacaggag aatttttgct gtttttcgat cacgatgatg agttgacgcc
    35881 ggatgcggtc ggcgaagcgg cactgtatct ggcggaacac cctgagattg atgcgcttta
    35941 ttctgatgat gacaagatcg attcggcagg aaagcggtat gcgccgcaat tcaaaccgga
    36001 ttggtcgccg gagttgctgc tttcctacat gtacatgagt catatcttcg tcgtgcgacg
    36061 atcgttgttt gagaccgtgg gcgctatgag gcagggattt gaaggcgctc aggattatga
    36121 tctcgcgttg cgggtggcgg agcggacggg catggtcggg catattccga aagtgctcta
    36181 tcactggcgg gcgttgccag gctcgacggc ctccagcggc gcagcgaagc ctgccagcat
    36241 tgaggcagga cggcgggctg ttgccgaagc atttgagcga cgaggcattg cggtgcgggt
    36301 gacgcaaccg gaatttgcgg cggcagggaa cctggggatc tattgtcacg aatttccaga
    36361 tgatggaccg accgtgacga ttctcattcc aaccaagaat caggtcacgg tgctgcgtca
    36421 atgcgtcgaa tcacttcgcg cgaccaccta tcgacagtac gagatcgtca tcatcgacaa
    36481 tgagagtgac gaccctgaga cgttggcgta tctgcactcg cttccccatc gagtcattcg
    36541 acttggtaat ccttccggcc ggtttaattt cgccgccatc aataatcgcg ccgtagagca
    36601 agtcaccggt gagttcattc tgtttttgaa caatgatacg gaggtcaagt ccccacgctg
    36661 gttgagccaa atggttggat acgcgcaaat gccaggggtc ggggcagttg gtgccaagct
    36721 catctttgcg gacggtcgca ttcagcatgc tggagtcatc catgggcttt accatggcat
    36781 ggccggtccc gccttcaagc tgacgccctc gtgggaacac ggttatttgg cctatgcctc
    36841 ggttgtgcgg aattattcgg ccgtcaccgc agcttgtttg ttgacatctc gccggcgctt
    36901 tctcgatgtg ggaggtttca atgaggccga gttcggtgtc gcttataacg atgtggacta
    36961 ctgctatcgg ctggtcgacc aaggatatcg ctgcgtttac tgccccgacg cgcaactcat
    37021 gcactatgag ggttattcaa gaggattccg tgacgatcct gctgaggttg ccgcgtttaa
    37081 gcagaagtac aaacggcggc aggacccctg gtatagcccg catttgtccc tggtcgatga
    37141 gagtttcaac ctcgtgccgc gcgccgtcgc agcgcggcga cagaagccga ttccgatggt
    37201 gatgaccgcc ttaagcttga actgcgaggg agccccgtgg agccaatacg aattgacgaa
    37261 ggagctggtc cggcggaagg tcattcagcc cattgtgttt tcccctctgg atggtccgtt
    37321 gcggtccctg tatgaagaag agggcattcc ggtcatggtg gatcggcatc cgctctgggg
    37381 tgtgacgacg ctgaaggaat atgaggaagc cgtgaaagtc ttctcctcga agtgtctgtc
    37441 gtggggagca gaactcatat atgccaacac gctccaaagt ttttacgccg tcgctgctgc
    37501 gcagatcgcc ggcatcccct ccgtatggaa tcctcgggaa agcgagccct ggcagacata
    37561 ttttgattat ttgcctgacg gggtcattca aaaagcctac gattgttttg catcgccata
    37621 tcgcgtggtc tttgtcgcgg atgccacccg cgacgcctat gcggcgctca atacgagaca
    37681 caattttacc gtcgttcgga acggcctcga ccagacacgc attcaacaga cgagcgaaga
    37741 atggcttcct gcacaggccc gtcagtccat cggcgcatgt gatggcgagg tggtgatact
    37801 actcctggga acggtctgtc ccaggaaggg gcaacaagat cttgtcaaag ccttggctca
    37861 actgtctccc gatctgcaga agcgggttcg ctgttacatc gtaggggacc ggccaagtga
    37921 atacagcgcg ggattgcacg ccctcgttgc gcaactgccg tcagagttga ggagccggat
    37981 gcatctcgtt ccagaaacgc gtcaaaccgc acgatattat cgagctgcgg acctgtttgt
    38041 gtgcacatcg cagctcgaaa gttacccgcg agtggtattg gaagcgatgg cctatggtct
    38101 cccgattgtg acgactcccg tatttggaat tcgcgagcag gtgcgagaag gagtgaatgc
    38161 tttgttttat gaagcgggtg atagcggcgc cctggctgat cgacttcggc gtctacttca
    38221 ggacgaggca ctgcgggctc gtttagcctc gaagagtgcc agcgtcctgg cgctcgggac
    38281 tgattttgat gaaatggtga gcagctatgc agagatcttt gccgaggcgt ggctgagcgg
    38341 agggccgtta tcatgaccgt acggttcaat ctggagacgc ccggcggtcc gcaattgaat
    38401 acgatcaacc ggttttctgg ttggtatatt ccagaccatg gtcgccgccc agagttgtac
    38461 gtggcctgta atggaaaacg agcggcgtcg ctggagtggg gaagtgtccg cccggatgtg
    38521 tcgtccgtct attcgactca ttcgcaagcg atcatcagcg ggtttcaagg cgatctattg
    38581 atgggggagc aggagcaagc tcaagccgtt gaggtcgtgg ttctcgatgc ggcagggcag
    38641 gatcgcgaat tatttcgccg cacatatgcg gtggggccgt atcgcccgcc cgttatacgg
    38701 gaccggtcgt tcaatctgtt tgagcttctc gtatgcccgg aatgccggtc tcctctggcg
    38761 aagcaggacc agggctggat gtgccctcgt tgtgccaggc tcgtgcccct caaggggggc
    38821 gttccacact ttcttcagga gcagggaacg ccctgccttc atgtcaccga gcagacgaat
    38881 actcatccct attctgccga tgtgctcgac atcctggggc gccaccgggc cgggttggta
    38941 ctggatttcg gggccggaca cacgccgaaa gaattgttgc gtccgcatgt agtgtacctt
    39001 gatgcggttc agtatgaatg gaccgatctg gtctgcacgc gggcaagact gccatttcgc
    39061 gatcactcgt tcgatgcggt ggtgagtcag gccgtgttcg agcaccttga ggatccgcat
    39121 tacacggcac gcgagctctg tcgaattctc cgcccgggtg gcatcattca tctcgacacc
    39181 gccttcatgc agccgttaca cggagaccct tggcatttct tcaatatgac ccagcatggc
    39241 ttgcggaaag tgatggctcc ctttgaggaa atccgttgcg ggatcaagag ctaccaacat
    39301 ccctccgcga gtctcttgat gcaattcgac gcgattgcgc cgttcatcac atcaagaaag
    39361 tggcgtaaca caatcgccaa gtggcgaaaa gagttggagg ccggggcagg ggagttcgat
    39421 gacgcactcg gggaggccgg gcgaactacc ttggcagcag ggtttttttt cgagggccgg
    39481 cgccctcaat aatggttgta gccaggggct gccacttggg ggcgtgccgg aagcgggcgg
    39541 ttgctccgtg aaaatgccgg acaggacgaa ataagtctct tgtgagtggg atttagggga
    39601 tgatggaggc aggtttcatg gagcagcgat tgcggagcat gttgcttccg gggatgaggc
    39661 ggctgggcct ggtgcccctg ctgaggaggc tccgtgcggg cttccgctct gctggaaacg
    39721 ctgaacccgc tggctccggc caccctggga acgatcatga ccgggtatcc gactattggg
    39781 gacaacagtt tcaagcgatg cgagtggaca attcgtactg gctcaataac aagctggttg
    39841 aagaggcgac ctatagattg atgaccgata ctccccggca ttggttgggg tggctcctga
    39901 atgactattt tgcccaacgc acgttcgatc gctctctcag cgtatgttgt ggagacgggg
    39961 cgcacgagat ccaactgtac acatcgggca aggtgcgttt cgtcagcggg gtggatattt
    40021 cagaaggcgc gatcaagcag gcggcagcga ggtttgccga ggccggagcc ccggcggact
    40081 gttaccgatt tgaggtgcgg gatgtcaacg cgctgcaact ggaggaccag tacgatctga
    40141 ttttttcgac cggcgcgctg catcatacga ccaatctcga ggcgctcctc tcaacggtgg
    40201 agcgcgcctt gacgccgaac gggtatttcg tggtggtgga attcatcggg ccaaatcggt
    40261 ttcagtggac cgaccggcag attgagattg cgaaccaggt actaaacgtg ctcgatcccg
    40321 cctatttacg ggacggcagg aataccagat tcgaaaggcc gaccatagaa aatatgctcg
    40381 cctgcgatcc gtcagaagcc gtgcgatcag ctgacgtata tccgctggtg aagaagtact
    40441 tcgatgtccg ttacgaacgc ttttataacg gcacgctgct ccatcaattg tacccgctcc
    40501 tacgaactga gttggcgaat caggggaagc gggactttga ttcgattgtg aaattgatcc
    40561 tcctgctgga agacctcctg atcaaaggca acgtgctccc gtcagacttc gtgttcctca
    40621 tctgccagaa acctgtgccg ggtagccggc gagcagcata accagggata tcaacgcatg
    40681 tgcggcattg tcggcgtctg tgatcgcatg gagccccagc aggcacacca tgtgctgtcg
    40741 cgtatgctgg caacgatgat agcgcgtgga cccgacggtg aaggtcggtt cgttgacccg
    40801 catatcgcca tgggcatgcg ccggttagcg gtgatcgacg tcgaaggtgg cggacagcca
    40861 cttttcagtc gagacggcca agtcgtagcc tttcagaacg gagaaatcta taaccatgct
    40921 gcgctccgaa gggagctggc acaacgtggt gtggaatgtc gaacggcaag cgacactgag
    40981 gtgttggcgc acggatatga tcattggggc atggatggac tgttagcgcg actcgatggc
    41041 atgtatgcga tcgccattct ggatcagcgt aatcaccagc tgcatctggc ccgtgaccgg
    41101 ttcggagaaa aacccctcta ctatagcgtc gcaaacggca agtttgcgta cagctcgaat
    41161 acgaccgctt tggcatggtt gccctggggc gatcgtgaca ttgatgcggg tagtgtcgac
    41221 cggtacctcg cgctgcacta cgtgccgggc cgccgtacga tgtttaaagg gattcaacgc
    41281 gtcctgcccg gtgaacacat cagtatctcg cttgattcgc tccagattat ccacgaccgg
    41341 tattaccgcc ccgcgatggg gccgattgag ccagtcgata ccgggacgct cgttggtcga
    41401 atcgaggctg cggttaccag ccggttggtc gcggatgtcc ccgtcggcgt ttttctgtct
    41461 ggcggtatcg atagttccat tgtcgcggcg ctcgccgcga agcatcatcc cgcaatcgat
    41521 accttttcga tgggtttcaa agatgcgcgt tacgatgaat cgccgcacgc tcaggcggtg
    41581 gcacgtgcca tcggttcctc acaccatcat ttcacatttg atgagacggc cttccacgac
    41641 ctgttgccct cggtcataga cgggttagat gaaccactcg gcgatcaggc attgctcccc
    41701 acctattggc tctgccggga agccaagcgg catgtaacag tcgtgttgtc cggggaaggt
    41761 gcagacgagc tctttgcagg gtatgagtat tacggtgctc tgttgtcgcg tcggtcgtgg
    41821 cgtgcatgcc tgaaagcctg ggtgatgggg cgaggtccag cggatgaact tcaacggctg
    41881 tgcgataacg tatggccggt gacgccatca ggttttccgc tcgtcatgga tacggctggc
    41941 cggcggcagc tacttggcgg gtatgcgccg gatcgcgatc cgtgggagga ggcgttgcga
    42001 tccacgctcc aacagtcggc gaacggtctt cagcgagcca cccttgccga tgtcctgaca
    42061 tggttgcccg atgatcttct ggtgaaactg gatcgcatgg ccatggcgaa cagtctcgag
    42121 gggcgcgcgc catttttgtc gccggtagtg gccgagaccg cgcttcggct gcctgaatcc
    42181 agtcgaatga cgccggtgcg cagcaaggtc gcactccgtg aggtcgccgc gcgactgctg
    42241 ccgcccgaaa tcgtccaccg aagaaagcag ggatttgtcc tcccgatggc gacatggttg
    42301 aagcaatggt tacgaagagt agagaacctg tatgaatatt ttgacctgca gcgtattgct
    42361 gtcttcgatg ggaacgctgc cgccactctg gttgaacgac aactgacttc tgatcgtccg
    42421 aatgagcgtc tgttattctc acttgtgatg ctggctgaat ggcatcattc gtttttccgc
    42481 cgcattcccc ccgcgaaatt ctaaacaacc gggaggacgg ctatcggagg cccgcggccg
    42541 atatcgagcc tctctgcccc cgtcaaaaac agcgaaaaat ctatccttgc cggagaactc
    42601 cggtactatt tgactagccg gttccgcctg cgcaaccggt ggatattccg agacaacggg
    42661 gcgaaacggc gcgccatgac ggtcacctat ttttctaaga gctcgagcat tggccccagc
    42721 agccgctatc gcgtgtatca atacttgccg catttgtaca atgcgggcat cgagtgccat
    42781 gtggagccgc tgttcggtcc cacctacttt tcgattctcc acattcgctc cttgatgctg
    42841 cagaccgttc tgaagattcc gtatgtcgtg gcgcgctttc tcaaacggct tggaacgctc
    42901 atgatgctgg gccaacggga cctgatcgtc atcgaagggc aactctttcc gtatgcgcct
    42961 ccactggtcg aacggttgtt gtgctggttg ggctatcgtt tggtcatcga gatggatgac
    43021 gcgatttacc tgactcgtgg ccatgaacgg aagatgcctg tgctcttcag gatggcaacg
    43081 ggcgtcattg tgggaaacga tcggttagcc gcctacgcca cccaatattc ctctaaagtc
    43141 acggtggtgc ctacggtggt ggacactgag cgatttgctc cgaggccctt gcgaccgacc
    43201 gccgcatccc atctgctcga tgaccctatc acgattgtct ggatcggcct ggcttacaac
    43261 ctcaaatatt tgaacgtgct tgcgcctgca atccgccggc tgcaagagcg ctatcgcgtg
    43321 aagcttcgcg tcgtttgttc tcagccaccg gtgctcgacg ggttggacgt cgagtttcga
    43381 tcctgggagt ggcggcgtga agttgaggat ctccaggatg ccaccatcgg cgtgatgccg
    43441 ctggaggata cagagtgggc ccggggcaaa tgtgcgctca agttgctgca gtatctcgcg
    43501 gtgggattgc cggcggtggc ctcgcctgtc ggggtcaatc gcgacattct tgtaaatggg
    43561 gacaatggat ttctcgcctc gaccgaagaa gagtggtatc tgcgccttga gtcattgtgc
    43621 cgcgatccgc aattgcgtgc tcgcatgggg caggcaggcc gccggaccgt ggagacccgg
    43681 ttctccttgg cggtatgggg acctaagctc gccgatgtgt atcgtgcgtt tgccgaggat
    43741 gcgcccagcc gtcagatgcc gcggcctgtc ggtctttctg tgcgaggaca tggccattcc
    43801 acaggtatgc gatgatcctc ctggcactga cattcctggt cgcggtgctg ttgtccgtct
    43861 atggcgtgcc tatcgcgcgc cgtgcggcgt taaaattcgg cattgtcgac agcccggatg
    43921 gtcggctcaa gcaccagggt gagccggtgc cctatttggg tggattggcg atctacctct
    43981 cctttctcgt gagcctggcg tttaccttcg aattccggca ggacgtgctc ggcatcgtgc
    44041 tctcaggaac gttgatcgtc atgctgggat tgatcgacga cttcggtgtg ttgtcgccgg
    44101 gcacgaagct ggccgggcag ttcctggcgg tcttcgtgtt gatcaagagc ggcattcgta
    44161 tcgagattgc atcgcttccc gattgggtcg atatcctgct cacggtgttc tggatgatcg
    44221 gcatcatcaa tgcctttaat ctcctggaca tcatggatgg attgtcagcc ggggttggcg
    44281 tgatcagcgc cgcgtttctg tgcgtggttg ccattctgaa cggcgatcag acgattgcgt
    44341 tcatgctggc ggccttgatg ggaagtctgc tcgggtttct tcgctacaac tggcggccgg
    44401 cttccatcta catgggggat tcgggcgcga tgttcatcgg cttgatgctt ggcgcattgt
    44461 cgatgattgg caaatacacg cagggccatt ccgtctcgtt gctgacgccg gtcttgatcc
    44521 tcggcatgcc gattttcgac acgctgttcg tcatgtacat ccgattcctc cgcggattgc
    44581 cggtattcct cggcagtccc gatcacatcg cgattcggtt gcgccattgg gggttgtcgg
    44641 tcacgcacgt cgtgttgctc agttatctcg gggcggcgat cttgggcggg atcgggctgc
    44701 tggtcatggc cgtcccgcag gaccttgccc tcatcttgag cggtgtcacg gtgttgtgtc
    44761 tggccgcggc tgctgtggct ctgacgcgag tcaatgtgtc cagcggggtc gtggcggtga
    44821 gccctgaggc cgtcccggca cgttcgacag aaaggaccgg cgcagtatga ttgtcatcgt
    44881 cggggcggga ttggcagggc tgagcgcggc ctatcacttg cgcggcatgc cgtacaagat
    44941 tctggagcgg gagcgcgagg tcggaggact ttgccgctcg tatgtcaaag acggcttcac
    45001 ctttgactac accggacatc tgctccattt tcgccagacc gcgattaagg cgttggtcga
    45061 aagcctgctt cccgaccggt tgcagcggca tgcgcgcaaa tcatttgtct tttctcatga
    45121 cacctatacg gaatatccgt ttcaggtcaa cacgtacggc cttcctcccg aggtggtgcg
    45181 ggagtgcctg ctgggattca tcgccacgct gtcacggtcg ccatcgacgc cgcccgccga
    45241 gaacccatcg ttcaaacaat ggatcatcga aagtctcggc gaagggatcg ccaaacattt
    45301 catggtgccc tttaatgaaa aattgtggca ggtgccgctc gatgagctca cctcggactg
    45361 ggtgtcctgg ctcgtgccga agccggatgt caaggacgtc gtcagcggcg ccttaggtat
    45421 taaggacaag gctttcggct acaacccgtc gtttcagtat ccggtgagcg gcggcatcaa
    45481 ggtgttgccg gaggcatttc tgccctccgt ggagaacctg acgtacggtt ccgaactggt
    45541 ggagatcgaa acggggaggc gtcgcgcggt ctttcgcagt gcgcaggggg aacgcattga
    45601 ggagtatgac cgtatcattt ccaccattcc tttacccgag ctggtgcgcc ggtgcgtcga
    45661 tcttccagcg tcgatgcggg agctggccgg cgcgctgcgg tgggtgtcgg tctataacgt
    45721 caatctggcc gtggcgcggg aacatgtgtc ggataagcat tggatttatt ttccggagca
    45781 ccggtaccca ttctaccgtg ccgggttccc catgaatttc tctccgtcca tggggcagcc
    45841 agggtgcagc tctctgtatg tagagatttc gcaccagccc acggaaaaag aatcggagac
    45901 atcgctgatc gaacgggtcc gccgtgggtt ggaaggggcg ggcgtgctgc agccgacaga
    45961 tgagctggtc atgtcggatg tgaaggactt gtattacgcc tatgtgttgt tcgatcggta
    46021 ccgcagccgc gccgtgaagg agttgttggc tgagctggag cgacgcggca tttcgtcaat
    46081 cgggagatat ggattgtggg aacacacgtc gatggaagac gcgattgccc aggggcagca
    46141 gcttgccatg cggttgcgaa tgagggccgc ggcctgaggg cgtcaatcgc atggatgtaa
    46201 tctgtcacgt catcaccaaa cttgagctgg gcggcgcaca ggaagtggcg atgcgggtgg
    46261 tgtcgggcct ggatcgtcgg cgattccgtc cggtgctgat tgccggcccc ggcggcttgc
    46321 tgaccgagga agcgcatgcg ctggaaggtg tcgatgtccg cttgatccca tcgctcgttc
    46381 gagaagtcca tcccctccaa gatctgcagg cgctgtttga actggtcgcg acgtttcgac
    46441 gcttgcggcc aaagattgtg cacacccaca gttcaaaagc tggtatccta gggcggttgg
    46501 ccgcctggct cgccggggtc ccctgtatcc tgcacacgat acacgggtat ggtgtgacac
    46561 ctgcccagcc gttgtggctt caacatctgg tgatcaagct tgagtggatg gtcggacgag
    46621 tgaccaccca ttggatcgca gtctcccagg ctgatcgccg acagggactc gagtgggagc
    46681 tgttcagcgc ctccaaggtg tcggtggtcc ggcctggcat tgatccgacg ccatttgccg
    46741 cccggattga gcaggcggag cgcgaccgtc tgcgagccgc gctgggagtg gggccggagc
    46801 agctcctcgt gggaacagtc tcgtgtttga aaccgcagaa atctcccgaa gactttatcc
    46861 gcgtcgccgc gctcgtctgt cagcgcatgc cagcagcaaa atgtgtgctg gccggagacg
    46921 gcgcactgcg gccgcagatc gaagcgatga ttcaagcgca agggctgcag gatcgggtga
    46981 gtcttctcgg ctggcggcgc gatgtggcct cattgctgaa ggcgtttgat gtcttcgtgt
    47041 tgacatctca atgggaaggg ttgccctgcg caattctgga ggcgagggcc agtcggattc
    47101 cgattgtcgc cacgcgagta gggggctccg ccgaagcgat cattgagggc atccagggaa
    47161 ccctctgtcc ggctggcgat gtcaaagcca tggccagtcg tgtgtgtcaa atcctgggtg
    47221 acgaacggct tcgagcggat ctccggaacg gggcgcagga gttgccggaa gagtttacga
    47281 ttcaagaaac aattaaacag tatcactcgc tctacacgta tctcctgcac gccacgcggc
    47341 cggcagagga tgtgatgaaa ttgcagccca atcgcttgta aagaggaaac cgggtaagac
    47401 gatgaatatt ccgctgcttg atttgaaagc ccagtaccaa tccatgcgca gtgaaattct
    47461 ggccgcgatc gaagcgacct gtgatgagca gggctttatc ctggggccgc gggtggtggc
    47521 gctcgaacag gcggtggcga cctatgtcag tagcacgcat gcggtgggcg tggcttctgg
    47581 cagcgatgcc ttgctgctgg cattgatggc gttgggtgtt aaagccggcg atgaagtcgt
    47641 cacggtgccg tttaccttct ttgccaccgc gggggctatt tcacgcctgg gtgccaaacc
    47701 ggtcttcatg gatatccgtc ccgatgattt taatatggac ccgacgttgc tggaacgagt
    47761 catcaccaaa cggaccaaag ccattatccc ggtgcatctc ttcggtcaat gtgcggacat
    47821 gggcgccatt catgacattg cgcgacggca caagatcggc gtcattgaag acgcctgcca
    47881 ggcgatcggc gctgcgcaaa acgggaagcg cgccggcgtg ctgggagacc tggcctgttt
    47941 cagtttcttc ccatcgaaaa atcttggcgg gtttggtgat gccggcatgg tgacgacgaa
    48001 tgacaaagag ttggccgaat ccatcgccat gttgcgggtc cacgggagcc gtgtgcgata
    48061 cgtgcatgaa gccatcggca tcaacagccg gttggacgcg ctccaagcag ccgtcttgtt
    48121 ggtgaagtta caacggctcg atcaatgggc cgatgggcgt cgccggaatg cagcgcgata
    48181 tcaccagtta tttaccgacg cacaattaac cgatcatgtg actctgcctg tgacgcatcc
    48241 tcataatttc catgtcttca atcaattcac gctacgtgtc aggaagcgcg atgaattgcg
    48301 ggcctacttg aaagatcatg gcgtcggaac ggaagtgtat tatccattgc cgctgcacct
    48361 gcaaaattgt tatcgcgatc tcgggtacca gaaaggcgct ttcccgcagt cggagcgtgc
    48421 cgcggaagaa gtgttgtcga ttccgattta tgcggaactg accgatgagc agcttcagta
    48481 cgtcgtgcag atgattgccg cgtttttccg taaacgataa cgccggcaga cttcgccagc
    48541 gtcgatcgcg ttccactcca acggcctgtt ccgtcggccg atcctcgatc tcctcaacgc
    48601 cgcagggtct cgtgcatgtg tcgtccatgc gcaggccccg caacgccttc tctccacaga
    48661 tacgtcgtgg gcaaccgcat gattgtgcct gcctagtgcg ccactccgat cagtcattga
    48721 atggacgaca accgcacgaa tcgcgcgccg ttcaagtctg aacattcatg aggcagaggt
    48781 gtacacccga atctcccaga agatgtggcc cggggaggcg agccgcgctg cactagctgc
    48841 gtgaaacgcc tcacctgccg cctgtcgcgg atacatggtt agccgggaag tcgcaccgag
    48901 cagggcgagg ctgtctgtac gtccgtacct ttcctcgaca gcacgtgttc acgcatgccg
    48961 ttatgaagcg tgtgagttgg aaggcatagg tattgctcct acacccagag ggaacgtgaa
    49021 acggcgtcgt tacaattatg acaattcctt cttcatactt tctgggcatg tcccgcagca
    49081 gctattcgat tctgggcgtg gcgctggcat tgattgccgt gatcggcgcc atcgattggt
    49141 ggctgcctct gggattgacg atcactacgc tctacgtagt gcctgtcctc attgcctctc
    49201 gcattccgta tccacgtctc acattctggg tggctgcgac cgcgtccttt gtcacgatcc
    49261 tggacctgtt tcacagcccg ctttacgcgt tgacgtgggt gagcgccgtg aatcgagcct
    49321 tcgccttgat ggtgatctgg gtgacggcct tgttgtgtct gcgtcgccag cgcgacgagg
    49381 cggagttgct gcgcatcaat gaagatatcg agcagcaggt gcaggaacgc acctcggatc
    49441 tggcggctgc caatcaagag ctcgaggtcc ttcgtgcgga agcggtgtcg gagttggtgg
    49501 caatcgtcaa atcgtccgac gacgctatcg tcggcatgac cttgaccggc attattcaaa
    49561 gttggaatcg aggtgcagaa cgggtctatg gatatagtgc cgaggaagtc ttggggcggc
    49621 cgatttccgt gctctgtcct cccaatcgat tagatgaagt gccgacgatg ttggaccgca
    49681 ttgctcgcgg tgagcatgtg cgcaatgtgg agatggtgca gcgccggaaa cagggagagc
    49741 gtattgatgt gtcgctgacg atctcgcctg tcaaggattc cgatggctgc gtcatgggtg
    49801 cgtcggctat tgcgcgcgac gtcacggtga aaaaacgcat cgaggcggct ttgcgtgaaa
    49861 gcgaagcgcg atttcggatg atggccgaca cggctccggt catggtgtgg atggcgggac
    49921 cggacacgca tattaccttt atcaacaaac ggtggctgga gttcaccggg cgcacgatgc
    49981 aggaagagat cggtgacaat tggtttaccg gcattcacgt agacgacctg gagcgttgcc
    50041 ggaagtccta tctggacgca ttcaagtcgg aacagccgtt ctttctcgag taccgcctac
    50101 ggcgccatga cggggagtat cgatggatca tggatacggg tgtgccactg ttcgacgagg
    50161 atggtcgatt cggcggctat atcggcacct gcatggatct caccgagcgc aaagatatgg
    50221 aggatcaact ccggcgcatg ctcaaagaga aggagagttt gctgcgggaa gtgcaccatc
    50281 gggtcaaaaa taatctccag gtcatctcca gtctgttgaa tcttcaatct gcctctatca
    50341 aggaccccgt ggtgaatcag ttgttccgag agtgccaggt ccgtatcacc tcaatcgcgc
    50401 tgctccatga aacgctacac cgttcgaacg atctctcgcg catcaagatg ggcgattata
    50461 cgcgcaccct gaccgggcat ctgttccgtt cctacggagt cgatccggga ctcatcagtt
    50521 tggagttgaa tgtagacgat gtcgagtttg atatcgacac gggtctgacg tgcggcctga
    50581 tcatcgatga actggtgtcc aattgtttga agcacgcgtt cgtcgagagc ggccgcggga
    50641 ccgtgcatat cgatctcttg gaccatgtgg acgggacatt caccttgtgt gtgagcgatg
    50701 acggcatcgg cattcccacg gatggggtgc tgaacaatcc cgattcgctg ggattggagc
    50761 tggtcacgct gttggccgag aaactggacg gccatacgga actccgaagc ggctcgggca
    50821 cggaatggcg aatccgcttc cagcaactgc aatattcaga aagggtatga cgccatggac
    50881 gcagccagca tcttgatcgt agaagacgaa ccggttgtgg cgaaggatat tcaactcagt
    50941 ttgcagcggc tcgggtaccg ggtcccggcg acggcaacct cgggagagga ggccatccgc
    51001 aaagcgggcg acacgcatcc ggatctcatt ctgatggata ttgtgctgaa agggaaaatg
    51061 gacggcgtcg aaacggctct tcaaattcag cgccggcagg atgtcccggt catctacctg
    51121 acggcctatg ccgacaacca tactctggaa cgagcaaagg tgacgtcgcc ggccggctac
    51181 atgctcaaac cctatcaggc aaatgaactg cgaacgacga ttgagttggc tctgcaccga
    51241 gcgcagcatg accggcacat gcgcgcacgg ctgcgatgga tcgcgacgac ggtgcgctgt
    51301 atcggagacg ggatcgtcac gacggatcgc ggcggccggg taacttatat gaatcccgct
    51361 gcggaagaat ttaccaaggt gacccaggat gaagccatgg gcatgagtgt gacggcgctc
    51421 atggggtttc ccgatgatgg ggcgactcaa cacggtggca atcctgccaa tcaggcaatg
    51481 gcggagatgc gcctcgtgac catcgatgag gcgacggtga tgtcccggca aggcgagccg
    51541 cggtccattc gcgggagtgt cgccccggtc gtcgatgacg gcggcgcggt gctcggggcg
    51601 gtgttcgtct ttcatgagcg cgttccgggt gagcggcacc ttggcccatc gaacgtacag
    51661 gctgaagcca tctggagaat ggaggagcgg cttggacggc cgcaggggat cattaatttg
    51721 tgttcgtggt gcaaacgggt gccggatcag tccggcgagt ggtacgactt ggccacattc
    51781 attgcggagc gttctgcgat tcagttcaat ggcgggctct gtccggagtg tatggaccag
    51841 tgctttccct gtaacggaaa acataagtag ccaaactcgc tcctggaatc cctgtactgt
    51901 cactcagcgc tattccgtgg gtcgacggcg agccttcgcg cctgtcctgc tccttgcact
    51961 aatttcgaag aagacttcaa gaacgcgccc gaatagacta tagtagcact gcacattcgc
    52021 agactacttg tgacgaaccc aagccccaac actccagtat caggcccgtt gcggatcgcg
    52081 ctcatcggcg ccggccgcca cgcacaacac catgcccgcg ccatcttacg atgtcccggt
    52141 gtgcagttgg tggccgtggc tgacccctcc gatgcggctc aagcggccat gcgcgacatc
    52201 gtgccgggta tcggctgctt taagacgcct gaagagctct ttgcttcgga aaaactgcac
    52261 gtcgtccata tcatcacccc gccggcgacc catgccccga tcgcgcgtat ggcactcaag
    52321 gccggatgcc acatttatgt ggagaaaccg tttaccgagt ccgtcgagga cgcccagcag
    52381 atcctggatg aggccagcgc aaagggtctt cgcgtctgtg ccggccacca actgctttac
    52441 gaaccgccga ctcgcgtgct gacgcaatac ttgcgctcga tcggtcgggt tgttcacgtc
    52501 gaaagttatt tctccttccg gaccgttcgg catgccccgg gcgggcgaaa ggtgcttcgc
    52561 gccgatcatc aattactcga tatcctgccg catccggtct atttgctcct acaagtgctg
    52621 gagcaagccg gagaagggcg cacggaactg ttgtcgctgg aagtcagtca ggccgggacc
    52681 gtgcacgcgc tggtgcgccg gggcggtgtc accggtacat tgatcgtgac gctggaggga
    52741 cgcccggtcg agagttatct gcgcgtgatc gggaagaacg gatctctatt tgccgattac
    52801 gtccgcagta ccacccagcg cgccatcggc ccgggatcgt ccggcattga taagctcttc
    52861 gcaccctatc ggcaggcgtg gcaattattg accggcacca cttctgcgat ggcgagccgg
    52921 tttctcaaga gccagcggag ttatccggga ttggccgagc tgttttcagc gttttacgaa
    52981 tcagctcgca ccggcaatcc gtcgccgttg tcgcccgaga gtttgctgga gactgtgcgt
    53041 atctgcgaac gggtggcgaa agcattgaag gtcggcgagg ccaaagccct ggcggcagcc
    53101 gccccgaaac cggtcgagag tcgcggcgtg ctggtgaccg gtggaacggg cttcttggga
    53161 aaggagatcg tccgtgcgct gttatcccgc ggacgccccg ttcgtgtagt ggcgcggcgg
    53221 gagccatctc cctgggagcg gatcgcgggc gccgaatatg tcgtagcaga tgtggcaacc
    53281 ggagccgccg ctcatctctt caagggagtg gatacggtca ttcatgccgc tgcagaaacc
    53341 gccggcgggt ggccggagca tcagcgtaat tccttggatg cgaccgagca tatgctgcgt
    53401 ggcgcggctg ccgtcggtat caagcacttt gttcacgtca gtagcctcgc ggtgctggcc
    53461 caaggcacgg gacggccgat tccggacgat catcccttgg aaccggacag caaggggtcg
    53521 gggccctatg tctggggaaa gttggagtcg gaacgtctgg cggtgcaact cgggaaggac
    53581 ctcggggtgt cggtgaaggt cattcgcccg ggagctctgg tggattatcg tgatttcgat
    53641 cctcccggcc gtctcggcaa gcgtctgggc aatatctttg tggcggtcgg ctcgcccggc
    53701 gatcggcttg gtgtggtgga tgtcggattt gccggtcgat tcctcggctg gatgaccgat
    53761 gcctgggaca tggcgcccag tccactgaat ttgctcgacc ctgtgtctcc gaccaaacgg
    53821 gagctgctgg atcgtcttcg ccaggccaat ccggacttga gcgtcttttg gttgcccagg
    53881 tttgtcctga taccgctttc atgggtggcg acactggcgc aaaaagtgtt gcgccccggg
    53941 aagccggcga tcgatgtggc caaagtcttc agcgtgttgc cctacgatac ctccggcatc
    54001 gctacgcttg ctcctcgcgt cgattctgct tcggaactcc atcgctgaac tttaccctca
    54061 cggtgtgctt cgctcggtat tcgtggcctc tattccacag tgtatgcgcc gatcttcaga
    54121 atggctaagt ctctgaaaaa ttgaatgaac cgtcgcttca agaaaaagtt gcttcctcgg
    54181 ggttgatttg agcccccgga gcgggtaagt ctaagggtat tccgcaggca aggtagtctc
    54241 caaacacaac cgcctcacca tcgaagagtt aacactatga acatgcgatc gaacgcgctg
    54301 gcatttacca ttggtctggc cattctcggc accctctccg gttgcgcgga agaccatcgc
    54361 tggcgtgctg cgatgaatga tggcaatacc gcgatgcggt caggcgacta tacgcacgcc
    54421 gaagaatcct ttgtggctgc gcgtaaagag gcggaagttc tcgatcccca aggaaagcgt
    54481 ctcgccgaaa cgctgagcca gctgggcgaa gtgaatcgtg aactaggccg gtatccccgc
    54541 gccgaagcgc tgttccagga agccttggca atcagagagc gcgtctatgg acctgctcat
    54601 gcggagacgg ctgcaagttt gacggacctg ggtgagctct accggctcca gggattgtac
    54661 acacagtcgg aagcgctcca tcaacgcgct agagaaattc gtgaaaaggt gttcggcccc
    54721 gatcacagca aaacggcaga aagcctgaat aacatcgccg tggtgtatca ggatcaacga
    54781 cgcttcgccg atgcggaacc agtgctgcag cgggcccttg ccatctttga gaaacagctc
    54841 ggcccggagc acagcttgac ggccatcacc cgggacaatc tggcgaaaat gtatcaggca
    54901 caggggcagc atcctcgcgc tatgccgctg tatcagcgcg ccctcacgat tcatgagaag
    54961 gcctttggtc ccaatcatcc gatcgtggcg agaaacctgg aaaatctggg tgacacctat
    55021 cgggcgcaga atcaatatcc tcaggccgaa gcgctctatc agcgtgccgt gagcatcttt
    55081 cggaagtcga tcgggagcga ccacgtggac acggctgagg ccatgagccg cttgggacaa
    55141 ctgtatgaac tgcagggact gtattcgcag gcagagccgt tgtttcagca ggcgattgcg
    55201 atccgggaaa aacagcaggg tgccgacaat cctcaggtcg cgggtgaact gaaaaatctg
    55261 gcgtcgctct accagtcaca gaacaagctg gatcaatcgg aggacctgta caaacaggcg
    55321 ttggtcatct acgagcaggc cgtcgggtat gatcgtgaag cctatgtgaa aacgttgaag
    55381 agctatgcct cattattgag acgccgccaa cgctccggcg aagctgaacg ccttgaggag
    55441 cgggctaaga gcgtattgaa acgtctttcg ttggaaagcc agagccaggg caaaacagcg
    55501 gcggacctgc ctcttgcgcc tgtgtctccg gtcccttagg agtgtacccc cagcttcctg
    55561 gactcaggac tcctccatcg gtgctcctgc ttctcctctc aacgcgttgg tccgaccccc
    55621 tccattctgc ttggaccggc cgggcccgcg cgcacccctt cggaagtatc gattgagacg
    55681 catcgtagaa tctcctacac tgtggccgcc gaaccggtaa gtgcgaccca cccagaggac
    55741 gttctatgaa tcgaatggtc atcgcgatag tagccgtggc gtgtctggtc aatgcagcct
    55801 gtgtgagtaa gcccccgaaa aaacagtttc agcggctcgc cggagagttc gtttcgcccg
    55861 tccatatcac caagccggtc gatcagcaga gtgccgtaat cggcatcacg atcaagagtt
    55921 ccgcatgggt gccgaataca gaggatcgcc tgtatctggt gaagatcgaa aatgaccggg
    55981 atttattcga aggggctaaa ctcattccga ccagtatcgt atctcctcct cccggagccg
    56041 gtggaggtgg aacggtgtat gtgcagaatg tgccgccggg ccggtatgct gcggtggcct
    56101 tcagtacatc ggaaaaggga ttcggtaagg tgcgggaagt gaccctcttt ctgttgccga
    56161 agtcggtcgt gaagcaaagt gataccacgg tgacggcggg aagcatgcat ttcatgggtg
    56221 agtacgaggt cggcgcgtcg acgatggttc ttcaggaaaa tgcagccgac gaggtgcagg
    56281 cccattactt cgaagcattc tggggaaagc cattggcgca agtgattgcc gatcttcagg
    56341 ttcttggcac ccccgcctat ttcgcggtgc atacggtcaa tgtgaaaggt ggaagccgtg
    56401 atgccgatgc cgaagcccgt tttcaggcaa cggcgaagcg cgatcttgaa ccttcgtggg
    56461 caccggtcat cgaacgggct cgagtgccga agaagtaaca acagcggagc gacgcgagat
    56521 acggccgcgc atcgtcagga cggtgcgcgg ctcttttttt gtaatgtatc cgatcggtcc
    56581 ccccgctaaa gctccagatc cgcaccgtat cttcccgcct ctcttcgcca tcatcgcctc
    56641 ttccgttaac acaatgttta cctactccgg aagtcgcggt gatcttcctc ctgtacgcta
    56701 gtcattgtcg ccgcatctca ccaccaatgc aggaggatca catgcaactt gtacagggag
    56761 gcctgcaaat ctatcgacct cgttcgcgcc ggcaggatgt gttgacgttt cgccatggcg
    56821 taccccagag caaccgcgtc tgccgggaat gtcacaacga ctttcgctct acggttgacg
    56881 tccgcaggtt tcaggcgaca ctctgcccgg agtgcataag tgagcgactc gaacgcctgt
    56941 ctgctgacgc cttataaggt cctgttgtca acgccaatct cgacgtcgct aatcgcgtac
    57001 gacgattcca tcctcgctgc cgatcatcct cattgccggg tcggtatgta acacatccgc
    57061 tcgcggtatc tcaggctagc gcctactcgg aacgttcagt tgaatgggga ggggggctgt
    57121 cccgctccgg ctcagtagag ggggacagct cgggagagag gtgcggtgag gtggcgcagc
    57181 tcaactccca tccctcgcgg atggtcagac ctgcttgcgc agcatcgcgc ggtgttcaca
    57241 ttccgctttg cagtccacgc aaagggtggc gtccggctgt gcgcgcaaac gcgggagggg
    57301 aatatcagtc cggcacagat ggcacattcc ataggacgca tgatcgagac gattgagtgc
    57361 ccgcgtaatt tgttggagtt tagcgtaggt ccgctgtctc atgagatcgc caagcgccag
    57421 gtcgcgatcg catgtcgcga tgtcaagaac atcggcgggc agttcggctt cggtttccgg
    57481 tggtctccag ctcgcagctt gccgcaagag gatgcgttgg tcttgaagca attgccggcg
    57541 aatggatcgg aacgcctggt gacgtgaacg tggtcggacg acatgtgcga cggccatggg
    57601 agcccctcca tcggtaaagt gactggtgcg gtcattaggc tatcgtgtgg acacgacagc
    57661 cagatcacag tcaggttaag aaggggttaa accgtcaggg gggataaaaa gttggaggat
    57721 tggcgaaggc cttaagtgac gagtcggttc atcagccatt gttgggctga acgtgcggct
    57781 cttcgtccgg gcgtttggcg cttgtacgtg ccgtctcgat gcagaatcca ggcatgggta
    57841 ttatcttcca ggtaaggacg gaggccttcc tcgatgagcc ggtggcgaag tatgggatcc
    57901 aggacaggaa acgcgacttc aattcgtcgg aaaaaattgc gatccatcca gtccgcgctg
    57961 gagagaaaca ctctgtcgtc cccgtcattg aggaagtaaa aaatccggct gtgctcgaga
    58021 aatcgtccga tgatggagcg gacacgaata tgttctgaga ggccggcgat cccgggtcgt
    58081 aaggcacaag gtccacggac gatcaaatcg attttgactc cggcttgcga agccttgtac
    58141 aacgcgcgaa taatttgggg ctccagcagc gcattcatct tggcgatgat atgggccggt
    58201 ttgccttgcc tggcacggtc ggtttcacgg gcgatccatt tgagcaaggt agtatgcaag
    58261 gtgaacggcg attggagcag gtgtttgagc cgtcccggtt gtcccagaga ggtcagttgt
    58321 tgaaagacgg tccgcacatc ccgcccgatg gccgcatcac aagtcagtag tccgaaatcc
    58381 gtatagagcc gcgcattgcg ggcgtgatag ttgccggtgc cgagatgggt atagctgcgc
    58441 aggagccggc cttcccggcg aagcacgagg ctcatcttcg catgcgtctt gagtcccacc
    58501 accccgtaca ccacatgggc tccggcttct tccaaatcgt gtgccagctc aatattggct
    58561 tcttcgtcga aacgggcacg caattcgatt accacggtga cttcttttcc gccgcgcgcg
    58621 gcatccacga gggattgcac gatggccgaa tcagcccccg ttcgatatag cgtctgcttg
    58681 atcgtaagta cgtgcggatc cgtggctgct tgacgaagaa actcggtgac cggcgcgaat
    58741 gattgatagg gatgatgcag caggatgtcg ctctgccgga tcgcgtcgaa ccaatcctcg
    58801 ctcggcaccg gagtggtcgg cacgccgggt gtgaacggct gaaacttcag gtcggggcga
    58861 tcgacgagat cgggtacggc catgagtcga tggagattga ccggtccatg gcattgatac
    58921 acgtctcgcg catcgagatg aaactgctca cggagaaaat agaccagatc cggcgggcaa
    58981 ttgtccgcca cttccagccg cacctcatcg ccgaagcgac ggtccggcag ttgaccttcg
    59041 agggcacggc ggaggtcgtc cacgtcttct tcgtcgacaa acaggttgct gtttcgcgtg
    59101 acgcggaatt ggtagcaccc cgtgacatgc atgccgggga aaagttgatg gacaaaggca
    59161 tggatgaccg aggacaggaa cgcaaaatca tgcggccagg cggcgttgcg cacaggaagg
    59221 tgaatcacgc gcgggaggga cctcggaacc tggacgatgg ccgtgccgtt ggggcgtccg
    59281 aatgcgtcgg tcccttccag cgtcaccaga aagttcaaac ttttattgag cagtttcgga
    59341 aacggatgcg cgggatccag cccgaccgga ctcagcaatg gaagcagctc gcgtgagaag
    59401 aagcggcgca tccagcgaat gtgcgatggc atccagtcgg cccgtttcag aaaccggatc
    59461 cgttcgtcgg cgagtttcgg gatcagcgac tggttcagga ccacgtactg ttcctgcacc
    59521 aactggtggg tgagcacggc gatgcgagcc aggagctccg atggcgtcag cccgtcggcc
    59581 ccacgctgct cgactccatg cgtgacctgt tccttaaggc cggccacccg cacctcgaaa
    59641 aattcatcga gattggaact gacgatgcag aggtatttga ggcgttccag cagagggacc
    59701 tttgggtcct tggccagatc gagcacgcgg cggttgaatt ccagaaggct gagctctcga
    59761 ttgatgaacc attcgggacg ggagagatcg ctcgtgaccg ggcggacgga tgacaccttc
    59821 ggtgccggtg tggctgactg aggctcaatg tgtgatgctg tggagcgtaa tgccatacgt
    59881 aatgggtgag cagtctacac cagcctggtg tgatgacaga tcaagaaacg gttaactttt
    59941 ccgctcactg tgcgggggcc ttgccggtgt cccgtttatc gggcacatcc aattcgagcc
    60001 gtggagacca caaaattgtc aaaatagcgc tgctgcacct tgctgaacgg gaccgacgtc
    60061 caatactgtt ccagatacac ggcattgatc ccgtactcat tatacatgcc gatccaattc
    60121 agattggtcc gttgcgcttc cagctgcccg tcgatccaat attccaggac accatctgcg
    60181 gcatcccgac tattcagctt catgtgcatt tcgatgcaga accaggtgcc gatgtgggct
    60241 ggattgaaca gcgccgtctg gccgccggcc gcgccaagcc accgtaggcg agggaaatcg
    60301 ttgtaggtcg tcgtgagcag cgtaccgaat tcatctgtgc ccgatgcggg atcgaggtag
    60361 aggcgatcag ggtctgatcc cggatgagcg ccgctccaca aatggccgat ggctgcctgc
    60421 gcaaatcgat tgctcgaaag gattgtcgct cgcgtcagct tatcggctcc gccaccggtc
    60481 cagcccggtt gcagccgtag gtcgaaccgc cagtagatgt cgcgataatt ggcggttccc
    60541 gcgtcgaccg gcttcatata ctcgcttgga gtcttgccga aggccagatg gagcgaccct
    60601 ccatgcggat cacccggcag gaattctgct cgcatgccgg tcgaggcgcc gactcccgct
    60661 ccggcttcac ggacgaagcg gccatgccgg atatcgtact cgaagtaatg gctcaggcgg
    60721 tcgatttcga aatcatcgca ccagatccat tctggatggt gactcttcca atctgcgcac
    60781 agagcctggg gtggctcgcc tgcatggggc ttgatcggcc cgaggaggca ggcggcggcg
    60841 cagagcgccg ccgcgagact ccatcgagac cgtagtgcca tggctagctg gtgaccgccc
    60901 cttcggaagc ggaggagacg tggcgggcgt actttcccat gaccccggaa agatagcgcg
    60961 gggcagggtg tttgactttc ttcagccgcg aggccatttc cttttgcgtg atgttaacgt
    61021 ccaagcgccg tttcacgatg tcaaaggtga tgacatcccc ggtcttcacg gctgcgatcg
    61081 gaccgccctt gatggcttca ggggcgacat gtccggccat cagtccgtgc gtcgcgccgg
    61141 agaaccgtcc atcggtgagc agcgcgactg aatcgcccag ccctgcaccg acgatcgcag
    61201 ccgtcacgcc gagcatttcc cgcatgcctg gtccaccgga tggtccctcg tatcgaatga
    61261 cgacgacatc gccggccttg atcttcccgg cctgcacggc tttgaaggca tcttcttcgc
    61321 gctcgtacac cttggccggt ccgctgaaat gcaggatgga gtgaccggcg actttcacga
    61381 cgcagccttc aggcgccagg ttgcccttca ggatgaccag gccgccagtc ggcttgatcg
    61441 gcttggacag cgggcgcaac acctgctggt cgggtttttc tgtagcggcc ttggcctctt
    61501 cgccgatcgt ccgtccgctg acggtaatct ggtccgggtg gaggatcttc gcatcgatca
    61561 ggcgcttcgc caccagggtc gtgccgccgg ccgcatagag atctgccgcg gtgaagtgcc
    61621 cacccggctt cagatcggcc aacaacggca ccttgcgatt gatcttatcg aagtcgtcga
    61681 tggtgagccg cacacccatt tcgcgcgcca cggccagcag atgcaggacc gcattcgtcg
    61741 aaccgccggt tgtcgcgacg gcggcgatgg cattttcgat cgagcggcgg gtgatgatct
    61801 gtttggggcg aatgtccttt ttaagcaggt ccatgaccat tttgccgcac tcgaacgcca
    61861 catcgtcctt gcgcacatcc atggccggga caccgttacg tcccatcggc gacatgccga
    61921 gaaattcgaa ggcgatggcc atggtgttgg cggtaaactg gccgccgcaa gcaccgggtc
    61981 ccgggcaggc atggtcttcc aggtctttca gttcggcatt cgtcatcttg cccgacgcgt
    62041 gtttgccgac cgcttcaaac acgtcttgga tggtgacatc atgtccctgg aactgtcccg
    62101 gcatgatcga tccgccgtaa atcatgagcg acggcacatt caaccggcag agcgccataa
    62161 cggtgccggg aatggtt
//
LOCUS       DAOQDS010000015        61553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_148_length_61553_cov_23.476503, whole genome shotgun sequence.
ACCESSION   DAOQDS010000015 DAOQDS010000000
VERSION     DAOQDS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 61553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61553
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148_length_61553_cov_23.476503"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..435)
                     /locus_tag="PK589_05290"
                     /pseudo
     CDS             complement(24..435)
                     /locus_tag="PK589_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            794..2278
                     /locus_tag="PK589_05295"
     CDS             794..2278
                     /locus_tag="PK589_05295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNJ19044.1"
                     /translation="MTHRMGKGSSPIGLKAFILFQIFRSFDKPLDMISLVSLPVALCG
                     LAASFFSFIVIFYRLQHQGIDLWHAGGLLFIIAVFCLACISLSAHYRHTRRIASKAFT
                     LQLTATGIALALLFLGAEVMIRVVAEQTPEETFVGDTVLLPREWQRVQAHRLADWERS
                     RQQSTIIEPDATLGWTIGPNRKEIGIFGEMYFSNAYGLRTLANETDPKATAPALRIAI
                     SGNSYAYGLDVTYDETWGHQLESRIGQHVQVLNFGVPAYGLDQSYLRYLRDIQDWHPD
                     IVILALISHDLVRSGLVYYLIGFQGGRVPGAKPRLSVVGEEPFALNLPLPSPERIYST
                     SSIEDLPFVDFDIAYRPADWKWHLYQYSYLLRFAVSWNPTSLWSPYELAPDVERVDKA
                     ILKSFVDTVRATGSTPLVVFLPEYGEFRHSVRSPSEQQLLGRRVLQEAGVKFLDLTAC
                     LELVNATSRFTLGWHYTAAANSAVAECLLDEVSRLVGVILEHKS"
     gene            complement(2637..3236)
                     /locus_tag="PK589_05300"
     CDS             complement(2637..3236)
                     /locus_tag="PK589_05300"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNJ19045.1"
                     /translation="MIKELLDAERVFVIDHFLSEEECAALIRRSEAAAYEPGTVGGVV
                     NENFRNNERVIVDDESLASNLFQRAQPFLPAVLDGHHLVGFNERRRFYRYQPGQTFQP
                     HRDGSYLRMKTGDKSEVTMMIYLNDGMVGGETKFYASADHAFLQPPAPYLSVRPKMGM
                     ALVFLHAIWHEGAVVQSGLKYVLRTDVLYGLHSAESRQG"
     gene            complement(3325..5526)
                     /locus_tag="PK589_05305"
     CDS             complement(3325..5526)
                     /locus_tag="PK589_05305"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HNJ19046.1"
                     /translation="MPMDQAFRLSSILLAGTGFASLTLAMALPLWLLVLAAGAFGMAL
                     LRVQSSGAISGAIGHLRFSALTWNIFLLLAFAGFWIDLFLISQEILPAGIHFLVLLLV
                     NKLSNLDQRRDFLHLYAISLIALLASAALTTQIWYAPFFFIYLVIGVWTLLLYHLLQE
                     REELTEPEPGHAHAEPSPVPLPRLTARFFWTTNAMAASAFGLTLLFFFLIPRVGVGFF
                     QNNHGENLRTTGFSEHVDLGVMGPVKQDPSIVMRVELPERTGHDAKWEPLYLRGVAYN
                     RYDGKSWSNSLPHRRMLTELPEGTFTLRTLASKPSPPARQLKLEILLEPLDTTVLFGS
                     PFPTAVKGHFLSLQSDLMGSLYLPFPLHTRSQYTVYSSPTSLNAAEKKSAALIYPDSI
                     LQQYLQVPAVNEQVLELARQVTRPATSVAQAVAMVHTHLLTHYRYNLDVPSLQSPHPL
                     EDFLLTRKTGYCEHYATAMVVMLRSVGIPARLVTGFLATEWNEFGGYYTVRQRDAHAW
                     VEVYFPQSGWITMDPTPPSPNAVGPTWWQSANSAMDSIRLQWDRLFVHYSAHDQFTVV
                     QGIREGGEAVRAGFSETLSALSAQGVAIVSHMVIRLTPSGVPHVVIALVLAAAAVYGG
                     IVILRRFRTRTTPRSPFSTQQQTVIALYTTMLQCCAQQGITKPASATSVEFLEQIRTR
                     WGEAWPLVDSVTRLYARVRFGEAALTEADLATAEVQLRTLRLLKRKTADSQ"
     gene            complement(5498..6640)
                     /locus_tag="PK589_05310"
     CDS             complement(5498..6640)
                     /locus_tag="PK589_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNJ19047.1"
                     /translation="MAFITLRTWLHQLSRHRAISVTTEGVRFLLLTLAVGIAAVNTGN
                     NLFYLLLAMMLSLIVLSGFLSEQCVRRLEFHRHLPDTLFVNQQATATLWIANRKSHLP
                     SISLRFLDVAAGQDHECGIHLTHLAPGASTLRAYPLLIKRRGRCQLDGIRVVTPFPFG
                     LFHKKAFYPLEASVIVCPALIPLPPMQVQALHAIGHDHALARRGHGNSLYNLREFRPG
                     DDSRAIHWMTTARTAKLMLKETEAEDQRIITLAVFTVAADAEVTTFERALSIAASLID
                     QLLKSGVRLRLLLGDQQEVMAEGNESPLHLFHALALCERRPVDDSTAVQQALISTLVR
                     SQDGPTLLIASWADPALQDLFPGVDQILTPHTHKDLFDADGSGLPA"
     gene            complement(6713..7654)
                     /locus_tag="PK589_05315"
     CDS             complement(6713..7654)
                     /locus_tag="PK589_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNJ19048.1"
                     /translation="MNSSQSIKLLQDNIAQVIKGKARAIELAVVCLLARGHLLIEDVP
                     GVGKTTLAHSLARSLDCSFKRIQFTSDLLPSDIVGISVFNRQKQAFEFMPGPLFAHIV
                     LADEINRTTPKTQSSLLEAMSEAQISVDNQTYPLQQPFMVIATQNPAEYHGTFPLPES
                     QLDRFLMRLCIGYPTPEEEKKVLDRPPSLHPANEIEPVLNAQHILDLQAQVDKVRMED
                     SLMDYLLAIVLATRQNPLFSLGVSTRGALALSKAAKALALARDRTYCLPEDIKELAPA
                     VLSHRVMLNRSQGARTKSVEHTERVIHDILESIPVPV"
     gene            complement(7809..9137)
                     /locus_tag="PK589_05320"
     CDS             complement(7809..9137)
                     /locus_tag="PK589_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19049.1"
                     /translation="MPVDSDSLAAFRKQVTSARSLDAPLWEASRHLVHSADWPATRTA
                     LVKAIHASSIPDGLKQTLVAALPVDLATEDRTSSAVLLKQLTGLPLTKALRALCVFFQ
                     VGRPAMAKWPLPTVTPDTIDRFVQTHHNPFDVLLEQLPASLLDLGSGDLSFAEELATI
                     YDPPLTAQHRPLLLHCLDRLDPSSQLGGPLHAPPLRLNRLRSKPGLEFRFYGDQDMFA
                     LDSLERDQRLAARYLIVTCWAPATPTFAYEPTRLSARCLAEELRRTKGDSRQIRHGKE
                     PALEVSHAGRNLLFPPWKFDIRGPLALLELMASRAALCVLGAVDSQVCWEILSQLIDD
                     PRVRPADVVLSAETLPAIFGETYRQLSALPVGGSCLLSDLTPLRKSFPSVLHTSPGHP
                     APYRFRQVKIQRGALFAGLPASSTARRFQGMSEETPPWLLTLVPEPVAIS"
     gene            9271..10287
                     /locus_tag="PK589_05325"
     CDS             9271..10287
                     /locus_tag="PK589_05325"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNJ19050.1"
                     /translation="MRTSLQHLREKVLNNLSGVIAILVLVVALGGCQAAGPVPRVTPQ
                     VWDAWSVGQILDAKTGHVVSTAEWLEGLAGYDVIYLGEEHHNRSHIESALTVLRTLVT
                     RGRRPVLAMEMFGWEGQPALDAYLETKESARPEFLERVGWKQNWGGAFEDYEPLVQFA
                     KDQRLPVLAMNPPKTLIRQVVKLGLAQAKEQPEWRQRGMEQETIVDDPAYRSRILSQL
                     QACHGGGAPEDYQTMYDASMVRDEGMAKTVAAAVNRVRAAGDPTQGPVVSYTGGGHVQ
                     YRLPVPNRVARRVEHGLKQVTIYMATLEQDRAAELYQSMQEGIADFVWLTPPGSQGAP
                     RRCR"
     gene            10558..11370
                     /locus_tag="PK589_05330"
     CDS             10558..11370
                     /locus_tag="PK589_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19051.1"
                     /translation="MISSIVEPNAFQIGRQYQTERRVQLTDASDTEFTSSVMGNSGLY
                     EQTIRLHNGHLEAKCSCTLHEQPICRHGVAALLEYQRWSKARVVPRARPAAPRLEAER
                     ATTKSAPSGDVKLSELNQFSDWMQRVVQAIQSGQPVPAQPDIEPGLVSTWTRIIQQLD
                     ERRRESEVAQIELDTDIRNREAVVARLTQDLEASAKEAKSLQVICRDLQREVDQHKAS
                     LSKSTELSRHVEQFEGEVRAIAGLLTEKGRRLEGLSDTCRDVATMLKALGKV"
     gene            11512..11688
                     /locus_tag="PK589_05335"
     CDS             11512..11688
                     /locus_tag="PK589_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19052.1"
                     /translation="MNKLIAGVLGGIGALQATNALANVSEGPPDYSGITGLYYVLIAL
                     ILAFGVYDTFFKKS"
     gene            complement(11751..12323)
                     /locus_tag="PK589_05340"
     CDS             complement(11751..12323)
                     /locus_tag="PK589_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNJ19053.1"
                     /translation="MAFGNSLTAGLGVSPEHTYPAQLQKQLDTLGLHYQVLNAGVSGD
                     TTAGGLRRVSWLLAGKPSVVILELGGNDGLRGLSLPETRSHLDTIIKRLKEARVQVIL
                     AGMKLPPNYGEEYTTQFEAMYRDLAKAHALALIPFLLEGVGGDQKLNQADGIHPTGEG
                     YRIVVQNVLQTLLPLLKSGESHSPTSKKKA"
     gene            12613..13320
                     /locus_tag="PK589_05345"
     CDS             12613..13320
                     /locus_tag="PK589_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ19054.1"
                     /translation="MMIATQQVTMQLEAGGETVRILDDITLEIPEKQTVAIVGPSGSG
                     KSTLLGLIAGLDRPTSGTIWLNGREITRLNEKAMARLRLANVGYIFQSFHLIPTLTAL
                     ENVSIPLELAGDRHARQRAMELLEAVGLGHRVSHYPVQLSGGEQQRVAVARAFACRPP
                     ILLADEPTGNLDSHTGQQVIQLIMALHRDAGTTLVLVTHDQQLAASMERVITLRDGRI
                     GSDQFMASSYDESGDRL"
     gene            13317..15989
                     /locus_tag="PK589_05350"
     CDS             13317..15989
                     /locus_tag="PK589_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ19055.1"
                     /translation="MRPFWFILAWRDLRSSWRHFVYFLACIALGVGAVVGVSLFSANV
                     ERAVLKEARGLLGGDLEIRLSRPLGGPGSTVLKDLTGRGIEVTRVDELVAMVARVDRR
                     EGGMDVTQLVELKAVEPRYPLYGVVRVEPDRPLTELLHPAGDRCGTICHGAVVQQALL
                     IRLGLGVGDAMKIGQASFRITGVIHTEPDRMANMFSLGPRVLISQEGLAAADLIKPGS
                     RMRERHLLRLPASMALSPLLHELRGRLGVESARVSSYRDAQPQLKQFLDQLARYLGLV
                     GLTALFVGGIGVALSIHAFVREKLPSIAILKTVGADTATIIYSYLGQAVGLGLLGSLG
                     GIGIGVALQTVLPQAVSTLLATDVLQQVEFTSVLSLSAVAPIGKGLGLGILTTLLFSV
                     WPLLTIRDIKPAAIFRREVERVGAPLVRREKALWKRAGQVMTADPLRTVTAAGIGIGL
                     ASLSMWQAGSLTIGALFIGGLVIAIAVLTLGAQALLRGLRALPVPRELSLRQALGNIQ
                     RPGGQTLGVMVSIGVGVMVILAIALLQHALVRQVGENRPSDSPTFFFIDIQPDQAQAF
                     GELVHRRTGDFAPDLTPLVRSRIHAINGRPVTDEREAQQDEPPAQSKEEKRKSWYVTR
                     EYVLTFLDQVPKDNKIVKGAWWKPGQVFSKPQVSVEEEAAKSLDLDVGSLLDLNIQGT
                     ILQAEVSSIRKVEWGNFSTNFYMIFSPGSLDAAPMTYVATVRVAPQDEVAFQSAVVAA
                     FPNVTAINIGEVLNSFARVLDRLSLAIRGVALFCLMAGALVMAAALAATRYRRLYEAV
                     ILKALGATRGLIARSFAAEYALLGCVAGMIGVALASLFSWAILRYILELPWALELPVL
                     GIGLGCTVLLTLLVGFLSTYRILGQPPLTVLRHE"
     gene            complement(16018..18792)
                     /locus_tag="PK589_05355"
     CDS             complement(16018..18792)
                     /locus_tag="PK589_05355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNJ19056.1"
                     /translation="MTRPQGDLPVTRRQAAALAVRAATHAALWLGAWPGIASLVFVPE
                     ARSQTTSTHNQQHPHNTMTTSSAIADPYRLPRHVIPTYYDLRMEPDLPSHSFTGHEIV
                     TLTVTEPTTDILLNATELEVSAATLSGEGQPPRTGTVRMDDEHQRCHITFPAVIPPGV
                     WKLSLAFRGTLNDKLRGFYRSSYKDDRGIAHSLAATQFEATDARRAFPCWDEPHFKAV
                     FAVTLAIDPALTAISNTRIVDERLENGKRIVRFAESMKMSTYLVAFIVGELVSTAPIM
                     AKQTPVRLWSVPGKQHLTPFGHEIAVYSLNFLAEYYDIPYPGDKLDLIAIPDFASGAM
                     ENLGAITFRETALLLDQRTATHAEQGRIADVVAHENAHMWFGDLVTMAWWNGLWLNEA
                     FATFMEMLVVDAWKPEWERWTAFGMARAAALSVDGLLSTRPIEFPVRAPKEAEAMFDV
                     LTYEKGASVLRMLEQHIGPTVFRDGVRHYLHAHAYGNAETTDLWVSLGHASKQDVPAL
                     MNEWIFSPGYPLLSLSVESRSTLMLTQRRFMYADDSSTAPSGTASPRWQVPVQLRINT
                     ARGTETRRVLLSERENRIPLPEGWTSILANEGGHGFYRVRYSADLLSALQKDGLNTLT
                     AVERFNLLNDTWAATVAGMVSSADYLALTEHFRSEQDPHVWAVMLGSFSTINHLLEEE
                     DRPLLAALVRDRVAPLFQQLGWSPHADERDLVKELRGDIIRALGTLGRDETIQAQARE
                     AYTAIQQKSRPIDPNVIPALVSILAFTGDEARYEEFSGRFRQATTPQEERRYLFSLAA
                     FRRPELLERTLTKTLTDEIRTQDAPFLVSSLLQNVYIREKAWKFVKSNWERMDKLFPK
                     SGLRRMCGGLTGLSTPELEQDVRHFFTSRKIDLGGKTLEQNLEQLHIAVQFREREREA
                     IRAVLARRST"
     gene            complement(18789..19493)
                     /gene="rpiA"
                     /locus_tag="PK589_05360"
     CDS             complement(18789..19493)
                     /gene="rpiA"
                     /locus_tag="PK589_05360"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNJ19057.1"
                     /translation="MTTTSCDLDSEKRQAALKATEFVHDGMVVGLGTGTTSKHLIIAL
                     GERVRAGLNIQAVPTSHDTAALAKQSGIPLIESDNAWMIDVAIDGADQVDPALNLVKG
                     GGGALLKEKIVAAAAKRFIVMVDHTKLVPALGGSFPLPIEVVPFGWGSTARHIEAVSG
                     GRAVLRQRNGDVFQTEAGHVILDLHMPIIDDPAALEVELNQIPGIVETGLFIGRTSIL
                     IVGHGQGTDVTHAPKP"
     gene            complement(19490..20575)
                     /gene="glk"
                     /locus_tag="PK589_05365"
     CDS             complement(19490..20575)
                     /gene="glk"
                     /locus_tag="PK589_05365"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HNJ19058.1"
                     /translation="MILAGDIGGTKTNLALYEWTSGRVEPVREDSFHSADYKSLEEII
                     DEFLSTPLVKPALENALTEESVDLGSDVAAETAPVVPEPITLTAACFGVAGPVIDNRC
                     RTTNLPWFIDGAALGERFVIPHVRLLNDLEATAHGLLHLTTDEMVVLNAGAPPKKKQA
                     LALIAAGTGLGECILYWDGTRYRPMPSEGGHTDFAPNSDSEIELLRHLRGSYLHVSYE
                     RIVSGPGLHAIYEYLRDTKKNEPTWLAEKIKVGNPAAEIAEAGLKGQAEIATQALELF
                     ASVYGAEAGNLALKALTLDGVYVAGGIAPKLLKKLQDGSFMRGFTNKGRYKRLMNQIP
                     VKVVMNDKTALLGAASVAAQLTLPPSL"
     gene            complement(20584..21354)
                     /gene="pgl"
                     /locus_tag="PK589_05370"
     CDS             complement(20584..21354)
                     /gene="pgl"
                     /locus_tag="PK589_05370"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNJ19059.1"
                     /translation="MAHPPERYIFADAQEVVRAAATLFVTVGQQAISKRARFLVALSG
                     GSTPKALYMVLASKEFATQLDWNKVHFLFGDERAVPPTHQDSNFALANTLLFTPLNIS
                     ATHIHRMRGEDSPETAAIHYENLLRGLTATTSEQWPVLDLVLLGMGEDGHTASLFPGT
                     PALSEPTRWVVPGLAPQGTRSRITLTLGVINHASVVLFLVTGLNKATVVRRIFESRAD
                     APDAYPAARIRPETGRLLWYFDRAAASALTEATDELSS"
     gene            complement(21356..23056)
                     /locus_tag="PK589_05375"
     CDS             complement(21356..23056)
                     /locus_tag="PK589_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNJ19060.1"
                     /translation="MPIRPKVTSLLDTQTLATTFDELASSRAIERILMRDHTLWKPNP
                     TEIDNRLGWLTVLDHMQDGLSDLRSFAQAAREARTTDVVLLGMGGSSLGPEVLRCTFG
                     SSKGAPRLWVLDSTVPGWVRQVTDAIQPARTLFLVASKSGGTIEVMSLFAHFWELVHR
                     TKGHRGGAQFVAITDPATGLEELARERGFWRTFTNQPDIGGRYSVLSYFGLVPAALLG
                     LDVGKLLTRALAMRESCRHTVAKENPGSALGGLMAAMAKSGRDKVTLLTSPALTSFGL
                     WVEQLLAESTGKEGTGLIPVAGEPFASPAAYGADRIFISLRLRGDRNASLDRTVSALQ
                     RAGQPVYSIPLKDRYDLGGEFFRWEFATAIAGHALGIHPFDQPNVQESKDNTGRVLKE
                     VETQGKLPSIAVLPPKQALTQLLEQMGSNRYLAILAYTTPTTQVEAALRALRKALMVR
                     YHVATTTGYGPRYLHSTGQLHKGGPNSGLFLELVDTMKPDLAVPEKFYTFGTLAQAQA
                     LGDLQSLHTHQRPAVRIALGPNPVRTIRSLVTFLTPAKATRRKSVPKKRTTSTRRSRR
                     "
     gene            complement(23244..24395)
                     /locus_tag="PK589_05380"
     CDS             complement(23244..24395)
                     /locus_tag="PK589_05380"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNJ19061.1"
                     /translation="MMSRSRYICRIATRVTLVALLSMSLGGCVETVPEELVESIESLD
                     RDLSTLRASDVNPAAYSKFSRQWIALQGRIRSEENIVRWPWEDNELETDLEALQVEGT
                     QLLSDVNSRIQAQRTTAETKLAKIEQRLRLLNSRVGDIGSRVVLGEHPVETEVHVKQA
                     RLFLEQGQFEHSIQASERAGRILLTQTALLTNELGRYADDDLIAAWRESAQRTIDWSR
                     THRAQAIVVSKADRSLTLYKNGRKVLTYPIRLGSRGIRAKQHYGDGATPEGEYRIQRK
                     RGPGQTPYFRALILDYPNIDDRRRFEEAQKAGLIPKSQHMPGLIQLHGIAQGISDQPY
                     GSIVLDNPQIAQLFDQVAVGTPVNIVGALESQNSISLVLADLGDQEEET"
     gene            24886..25023
                     /gene="rpmH"
                     /locus_tag="PK589_05385"
     CDS             24886..25023
                     /gene="rpmH"
                     /locus_tag="PK589_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNJ19062.1"
                     /translation="MSFTFKQPSNLKRKREHGFRKRMSTKNGRKVIARRRAKGRARLT
                     V"
     gene            25517..25780
                     /gene="yidD"
                     /locus_tag="PK589_05390"
     CDS             25517..25780
                     /gene="yidD"
                     /locus_tag="PK589_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNJ19063.1"
                     /translation="MAQHIAKTAVGSCWGSLVVRYFCIGLLSVYRACVSPMLGPACRF
                     HPTCSVYAQDAIERYGLLKGVAMAGWRLLKCHPFHPGGVDPVR"
     gene            25826..27571
                     /gene="yidC"
                     /locus_tag="PK589_05395"
     CDS             25826..27571
                     /gene="yidC"
                     /locus_tag="PK589_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNJ19064.1"
                     /translation="MEKRVIVFLIVSLAVIIGYDYLLKGMGLLPPSEPVQVATNTQVA
                     ERQSAGSENASAQSVSPASSTQTSSPSAPGASDGGTGTVKEEQTIEVDTDLFRAKFSN
                     RGGVLKSWELKRYTTSTDQGPVPVQLVYAGGRFKGPLSLVTTDQSVTSDLGSALYQVT
                     QDISHLDSAHPTGHITFRYHDTQRNMDVEKELTFHHGSYVVDIAVRTQGLATPVDVTL
                     GTNFGIVEWGEGFIGLMGSASLVDDKVLKETPDGEAERKGDVKWSAIQDKYFLSVLMP
                     KKAMSALAKKEGDKLVSAGVRFPAPTPGSTMTMQLYAGPKEYDTLKGLNVGLEDTIDF
                     GWFVFGSWALVKAVAKPIFYVLRFLYEFTHNYGVTIILLTMLIKLMFVPLQYKSYKSM
                     KQMQVIQPKVLAIQNKFKDDRERLNKELIKLYKDHRVNPVGGCLPMVLQMPVFVALFN
                     ILYMTIDLRQAPFMLWIKDLSVQDPYYVLPIIMGATMVIQQKITPTTMDPTQAKIMLF
                     LPVFMTFLFVNFPAGLVLYWLTNNTLTITQQVVTERLFGKKWQVVPEGADAAAIEDSK
                     EQKSNGKRRNASATE"
     gene            27593..29023
                     /gene="mnmE"
                     /locus_tag="PK589_05400"
     CDS             27593..29023
                     /gene="mnmE"
                     /locus_tag="PK589_05400"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNJ19065.1"
                     /translation="MLGALDDTICAIATPPGEGGIGILRISGFHALDVASHVVRLRSG
                     NALQDLPTHRLALADICADNTDVPAAGTKRTGALRFIDEALVVVMKGPHSFTGEDVVE
                     VQCHGGPVILDQLCRGLISAGARLAAPGEFTKRAFLNGRLDLAQAEAVLDTIRAKTAR
                     SLTLAQSQRRGELSRHVEEIRSELVVALAHIEAALDFAEEDISFVRQDELLRLLDVTL
                     QKLRRLLQSGRDGRIWREGAVVAILGRPNVGKSSLMNALLRSDRAIVTAIPGTTRDLL
                     EESLSIDGIPVRLLDTAGIRVTDDPVEVEGIRRSRLAWTDADLALILLDGSQSLSESD
                     RALLSEPDSTRALLIINKCDLPGQLSREDLDRACPGHSGILEISATLSIGLDELRETI
                     RGRLMPGMWESNEGVLVTNLRHTVAIERALQGVDQARQSVEAGRAGELIAMDLRIAAD
                     ALGEITGVITTDEILDRVFAEFCIGK"
     gene            29037..30917
                     /gene="mnmG"
                     /locus_tag="PK589_05405"
     CDS             29037..30917
                     /gene="mnmG"
                     /locus_tag="PK589_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNJ19066.1"
                     /translation="MSEQCDVIVVGGGHAGCEAALAAARMGARTLLLTMDPDRIAQMS
                     CNPAVGGIAKGHLVKEIDALGGEMGRNTDQAGIQFRMINTSKGPAVRALRAQCDKKIY
                     RQVMQDTLRAQPCLRIRGGTVDRILTHAGAVVGIVTDSGQHIEARAVVLTSGTFLKGL
                     IHIGLSHFPAGRAGEASAEHLSDCMRDFGFEVGRLKTGTPPRLDRDTIDFSVMELQPG
                     DDPPPPFSYRTNRIPLPQVPCHLTHTNTQTHDIIAKNIDRSPLFSGVIDGIGPRYCPS
                     IEDKVVRFADKDRHQIFIEPEGLDTNEFYPNGISTSLPVDVQAAILRTIPGLKQAKIL
                     KPGYAVEYDYFPPRQLHNSLETKLVQGLYHAGQINGTSGYEEAGAQGIMAGINAALKL
                     RGDAPLVLDRSQAYIGVLIDDLITKDAREPYRMFTSRAEYRLLLRHDNADLRLMDLGH
                     RIGLIPDSVHHKFQEKRLAIEREVQRLKDTRPKLTPDVRRLLEEFEIGPVTPTQSLAE
                     ILRRQDLTYGKISSVFGGCEIEDADIMDAVQLEVKYEGYIKRQQQQIQRSQKFEHRAI
                     PESFDYDDVSGFSAEVREKFKRVRPASVGQASRISGVTPAAISLLLVAIERHKRQPLT
                     AE"
     gene            31007..31651
                     /gene="rsmG"
                     /locus_tag="PK589_05410"
     CDS             31007..31651
                     /gene="rsmG"
                     /locus_tag="PK589_05410"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNJ19067.1"
                     /translation="MEHDASQFEVSFRRFAAELGIPVDSSCLPFFSTYLSELKKWNKA
                     INLTAIHDDQEVLVKHFIDSVAGVKVIENMDKCELLDIGAGAGFPGLPLKFVLPQIEL
                     ELLEPSEKKGAFLRYVAGSLGLRHVTVVPSKLEQYVRRPTRRLFDYIVVRAFKVDESG
                     QMVADLLKPAGKVILYRAERVPRGFRLGGLALVQEVEYELPSGYGHRVLSVFSK"
     gene            31723..32499
                     /locus_tag="PK589_05415"
     CDS             31723..32499
                     /locus_tag="PK589_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNJ19068.1"
                     /translation="MARIIAVANQKGGVGKTTTSVNLAAALAIEGGSVLLVDIDPQGN
                     ATSGLGVDAMSLGKTIYNALIVKESITDLAMPTAVNGLSLVPANSHLAGAEVELVNME
                     GREQRLKEALAEVADRYDTILLDCPPALGLLTINAMVAAHSVLIPVQCEYYAMEGLGR
                     LMESIERMRQSLNPSLEIEGIVLTMYDARNSLARQVVEQIRGHFGEAVYQTMIPRNVT
                     LAEAPSYGRPALLYNMASSGAQAYLSLAKEFVVHGEKSPR"
     gene            32477..33328
                     /locus_tag="PK589_05420"
     CDS             32477..33328
                     /locus_tag="PK589_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNJ19069.1"
                     /translation="MEKKALGRGLDALLPTGRNTADPERGDVQELRLEAVVPNRFQPR
                     QQFSEVELAELTASLKQNGLLQPILVRRKGDGIYELIAGERRLRAAKLAGMQKIPALI
                     RNVSDQESMVLALVENLQRDDLNPMETARAYQRMLNEFSLTQEAIAQKVACDRSSVAN
                     MLRLTSLPAEVQHMIETDQLSTGHAKVILGLMTPAAQISLATQIVSGQLSVREAERLV
                     QGHAEAKKAGKRPVRAPLRSDLEERLQKRLGTRVDVQKGRRGGKIIIHYFSPEELDGV
                     VEQLLNG"
     gene            33467..33943
                     /locus_tag="PK589_05425"
     CDS             33467..33943
                     /locus_tag="PK589_05425"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNJ19070.1"
                     /translation="MWIKDKPEGKRQAGQSEGSEMENLESTAPREGNEEINAFVGKGV
                     SFKGVISYNGTVRIDGNLDGEIHTEGVLLVGEDAVLTAKITAGTVVCKGKITGDISAR
                     EKVKLRAPAVVNAGVKAPLLAMEEGVVFNGTLEMSQTGTREPQGTSRGQGVKLVNG"
     gene            34069..34524
                     /locus_tag="PK589_05430"
     CDS             34069..34524
                     /locus_tag="PK589_05430"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNJ19071.1"
                     /translation="MWGETKKQSVAESDKFTFLGKGTSFKGIVTFDGTVRIDGRVEGE
                     VHTGGAVIVGESAVIKGVIAAGSVEISGRVKGSITAQQKVEIQKPGILIGDIQSPVIM
                     IEEGAHFHGMSNMGAEKWSEDEASEFGSPQDPGVRDLVAHRGKVKTQEP"
     gene            34557..35639
                     /gene="mnmA"
                     /locus_tag="PK589_05435"
     CDS             34557..35639
                     /gene="mnmA"
                     /locus_tag="PK589_05435"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNJ19072.1"
                     /translation="MTRQTVLLGMSGGVDSSVAASLLVQQGYDVHGVTLQVWEHEDEH
                     VAVSKRWEERGCCKIGIAKFVAQRLRIPFEVVDRREIFQQGVIDDFVAGYAAGTTPNP
                     CVRCNERVKLRSLYALAQERGMDYVATGHYARVQQAGGQWSLHRALDARKDQSYFLYR
                     IQADWLPRLLFPVGEMQKSDVWKEAETLGLPVEELKESQEICFVSQGDYRTFLEQERP
                     ELKQPGAFVGVDGEVLGRHEGIAFYTPGQRRGLGIAVGQRLYVQKVVPESGEVVLCAE
                     EQLVQSDCQVGDISLFDRSIGRQAVDVDVKIRYATPPAAATLLPSTNGTVQIRFHQPQ
                     RALSPGQSAVFYQGDRVLGGGIIQRT"
     gene            35888..36427
                     /gene="atpH"
                     /locus_tag="PK589_05440"
     CDS             35888..36427
                     /gene="atpH"
                     /locus_tag="PK589_05440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNJ19073.1"
                     /translation="MIKTAVARRYAKALFELLDASSIEPARLALNGLGEAFTESSALR
                     HAVASPMFPEATKLSVLIEVATKLGCPPVVKRFLDQLVKKNRVSFLPDIADAFAKLVD
                     ESKGTQQVLVSSANALPATEQDRITTRLRDLLKRDVDVTFHTEPEHVAGLHIRLGSTV
                     VDSTVRGRLSAMQRVLTKE"
     gene            36430..37947
                     /gene="atpA"
                     /locus_tag="PK589_05445"
     CDS             36430..37947
                     /gene="atpA"
                     /locus_tag="PK589_05445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNJ19074.1"
                     /translation="MQIKAEEISSIIKEKIKGFDQQVDVSQTGSVIQVGDGIAKVYGL
                     DGAMAGEMLEFPGGLYGIALNLEEDNVGAVLMGDDTGIKEGDPVKRTGRIAEIPVGEA
                     LVGRVVNAIGQPIDGKGPINSQHSSRIEVVAPGVNTRQSVREPLQTGIKAIDAMIPIG
                     RGQRELIIGDRQTGKTAIAVDTIINQKGLGVFCIYVAVGQKRSTVARVVKTLEENHAM
                     EYSMVVAATASDPAPMQFLAPFAGAAIGEYFRDNGKHALIVYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDKLGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLGSDLFYSGIRPAINVGLSVSRVGGSAQIKTMKQVAGTLRLDLAQYREM
                     AAFSQFGSELDKATQMQLARGVRMVELLKQGQYKPMPVADQVLSIYAGVNGFLDDVPV
                     DKVLQFEADLLHYVAQNHQDMRKDIATVGKIDDKVGGKLKEIITTFKQKMGYGGK"
     gene            38030..38914
                     /gene="atpG"
                     /locus_tag="PK589_05450"
     CDS             38030..38914
                     /gene="atpG"
                     /locus_tag="PK589_05450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNJ19075.1"
                     /translation="MPSLQSLRRKIAAFKNTQKITKAMKMVAAAKLKRSQDRILAARP
                     YAHKMRGVLSNLSQRVNRTSHPLLQKREGKKIEILVVTSDRGLCGGFNGNIVRKSAEF
                     VRQCESQGLSVNLSLIGRKGRDYFRRRSLPIRQEWTGIFDKLSFEHAIDIGGDLTENF
                     VKGTFDELYVVYNEFKSAIQQRVIVEKLFPIDAATEFGASQATEGSSLGGSYLYEPDE
                     GELLSALVPKHFQIQTYRILLESAAAEHGARMAAMDGATRNAGQLIKKVTLYYNKTRQ
                     AAITKELMDIVGGAEALK"
     gene            38982..40424
                     /gene="atpD"
                     /locus_tag="PK589_05455"
     CDS             38982..40424
                     /gene="atpD"
                     /locus_tag="PK589_05455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNJ19076.1"
                     /translation="MSTGKVIQVIGPVVDVEFPPGQLPNIYNALKVTQEENKAAGKPA
                     VRITLEVASHLGENRVRGIAMSTTDGLTRGMDVTDTGAPIAVPVGRETLGRLINVLGE
                     PVDEKGPIKTNKTYPIHRPAPRLEDQDTKTEVLETGIKVVDLLEPYSKGGKVGLFGGA
                     GVGKTVIIMELINNIALHHGGFSVFAGVGERTREGNDLWHEMQESKVIDPDDHTKSKA
                     ALVYGQMNEPPGARLRVALTGLAVAEFFRDEENQDVLLFVDNIFRFTQAGSEVSALLG
                     RMPSAVGYQPNLSTEMGALQERITSTKKGSITSVQAIYVPADDLTDPAPATAFAHLDA
                     TTVLSRQLAELGIYPAVDPLDSTSRILDPQVIGEEHYKVARGVQSVLQRYKDLQDIIA
                     ILGMDELSEDDKMVVARARKIQRFLSQPFHVAEAFTGAPGKYVKLKDTVRSFKEILEG
                     KYDHLPEQAFYMVGPIEEAVAKAEKMGVKV"
     gene            40538..40960
                     /locus_tag="PK589_05460"
     CDS             40538..40960
                     /locus_tag="PK589_05460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNJ19077.1"
                     /translation="MAGKILLEVVTPEKLLLSQEVDEVIAPGSEGEFGVLPGHCHLLS
                     SLRIGELRYKSQEVWHYMSILWGYAEVTPVKVTVMAEIAEKAEDIDVGRAQQAVEKAE
                     QRLQAGGLPSEVREAEISLEKARLRKKIADRARKTGHA"
     gene            41124..41915
                     /locus_tag="PK589_05465"
     CDS             41124..41915
                     /locus_tag="PK589_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19078.1"
                     /translation="MQQRSDWFVRLDSVQSTEPSPVVYDHPFSWKAEELSAILSRLFL
                     EERVGLMDNAKPAKPVFTPVEIDLVEPAVREAFGKARPGEWIAFLLLQPNAGATGVTS
                     GGLFLESHRLHVVVANHRTMVGNNSDELVRVRANPFYSIQGSGGVLAFEPSRFRLSTK
                     ANWSGGHRASASELVLDHSAFLSYVTHAAPREMPTSAPSPIRQQSSAEALTSPADASV
                     QGGPHDQPMLPQIQGEIERLKQELAEKDLEIDRLKAQLLKSGARP"
     gene            42071..43018
                     /locus_tag="PK589_05470"
     CDS             42071..43018
                     /locus_tag="PK589_05470"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNJ19079.1"
                     /translation="MQTEFLISPGEQPKRLDIFLVNREPKLSRAAVQRMISAGRVRVN
                     NQTAKPSQKIKPGDVVNLDKPKPTPLLINGQPSALEILFEDSACLVINKPAGVAAHPG
                     PGHWDDTLLNALLDHALRAGVVGMSGLVHRLDRDTSGVMVVAKTKTAHGRLAVQFEQH
                     SITRQYEALVQGVPQHSHGRIELALGTDRNNVKRTSPHTLHPKASLTEYYVEEVFGAA
                     AAHMVLTPRTGRTHQIRAHLHAIGHPILGDPAYGAEPVRALEGYGVPRVMLHAYKLGF
                     HHPLTGRYCEFTVKPPADFRGLSDELRFKTVREGRGRTQ"
     gene            complement(43067..43552)
                     /locus_tag="PK589_05475"
     CDS             complement(43067..43552)
                     /locus_tag="PK589_05475"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ19080.1"
                     /translation="MSSNFIHRILRSLRSSVDPTPSVEPLVVDNITDLPLTEVLRERR
                     LDARFAVRTPCLYELDRKQQRETPVISGKAHSLNVSSGGVLLLLDQQPQTGQVLSLHN
                     PTLHSQPGTSLFEVRWTTSLPLGAQQGCYLVGCHLAFGRFPFFLVQRQYLHRDISSLS
                     I"
     gene            43934..44917
                     /locus_tag="PK589_05480"
     CDS             43934..44917
                     /locus_tag="PK589_05480"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNJ19081.1"
                     /translation="MNRVSTTPVEIVVTGGESSKRIDVFLANRDPTFSRSALQRLIGE
                     GRIQINGQPVRPSQKIRPGDRIRLEVPRPEPLDLKPEAIPLEILHEDSDLLVLNKQAG
                     LVVHPAPGNWSGTMVNALLHHFATAGVTPSHIGGKERPGLVHRLDKETSGVMVIAKTD
                     QAHRALAAQFKLHTITRVYEALIWGVPKKGHGLIELAIGRDTKERKKFSARTTNPKAS
                     ATDYQVEERYGKIAAQVRLSPRTGRTHQLRVHLTSIDHPILGDKTYGGAKVMTVAGIP
                     IPRVMLHARTLGFTHPTGGEYHEFDVPFPPDMEQVQHLLRDATTQGGGCRQ"
     gene            44980..45402
                     /locus_tag="PK589_05485"
     CDS             44980..45402
                     /locus_tag="PK589_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19082.1"
                     /translation="METSAVLDRIGELIAQLKGYAAKLPTVVHLASVPTGVKPKPEAV
                     EAFEQALLRFREQSGRSPFKGLQEYLLESLEAFEVGNVLGSVQPLMAVLDQVERMQRD
                     KEIKVTPAEDKRMGEYRAALIKILPGNQPELEGAGRGM"
     gene            complement(45491..45670)
                     /locus_tag="PK589_05490"
     CDS             complement(45491..45670)
                     /locus_tag="PK589_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19083.1"
                     /translation="MGTQVRSCPKCFQLMWLKQDHYDVIDEETVRTKCPHCDSTVRFQ
                     LVTAGANAAGPKMGH"
     gene            45734..46174
                     /locus_tag="PK589_05495"
     CDS             45734..46174
                     /locus_tag="PK589_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ19084.1"
                     /translation="MPPALKTGQRPIAFSDRNDFDATQLIHLYRQAPWAKHRALEQAQ
                     AMLAKTDLVISAWDGPRLVGFGRVLTDYIFRASIWDVIVDRDYQGQQIGTEIVRRILD
                     HPTLQQVELFWLCTRMPGFYERLGFSAKEQTGMVWSRKQPSTQS"
     gene            complement(46274..47095)
                     /locus_tag="PK589_05500"
     CDS             complement(46274..47095)
                     /locus_tag="PK589_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNJ19085.1"
                     /translation="MLELLAYDFMQRSLLAAALVGSVCSVIGVFVVLRGLAFAGAGTA
                     HAAFAGVTLAYLLGLPPLSLAIVFGLATVWITGWVEEKGRMKLDVSIGILYTATMALA
                     ILFLGLMKTYNPEVYGYLFGSVLSVTTEELLTIGGLSIAVLGTILLLSKELYFIAFDQ
                     EMAAASGVPARQIFYLLLTLVALTVVIALKTVGAILVFAMILIPASTAYQLTHSLTQM
                     TLYSMLIGIFCAVTGVLLSYAFDLPSGPSIVLLATSLFFLAVCCSPKRLHRIQAE"
     gene            complement(47182..47964)
                     /locus_tag="PK589_05505"
     CDS             complement(47182..47964)
                     /locus_tag="PK589_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNJ19086.1"
                     /translation="MTHPIIRFDHATFGFPGTVALEDISLSIPESEFVGVIGPNGSGK
                     TTLCRAVLGLMAPIRGTLRVLDCACEELRCHHRALIGYLPQKGMLDRNFPVTVLEAVM
                     MGRYGALGLFRRPSGKDRDIARQALTQVGMDHHRDSALGALSGGQQQRVFIARALAQQ
                     PRILLLDEPTTGLDLTAQHSVVELIQQLHQELKLTILMITHDINMIRSRVDRLVLLKT
                     KLFAAGPPQDVLKPEILSQVYGKELVITDKDFIIVEDYHHHH"
     gene            complement(48022..48942)
                     /locus_tag="PK589_05510"
     CDS             complement(48022..48942)
                     /locus_tag="PK589_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNJ19087.1"
                     /translation="MFRTILLTLFIFLTMLGNLSAAVPTASAAEPLNVVVTLPVLKDW
                     VQQIGGSHVHVVSLMTGYESEHTYSPKPSDLVAVRKAALLFEVGAGLEVWVSSLVKNA
                     GNASLQVVTTSKDIELIPDHPESTADAHTHHHAGGNPHVWLDPSAAATMVQHISKAMV
                     EADPAHAAEYRNNTADYLQALLRVQAESLERLKKVPSRAVIVHHPAWPYFARRYDLQI
                     AGTILTQPGGEPSARHLHTLIDAIKRGHIRVIISEVQLNQKVPQLLARETGAHIAVLT
                     TLPGGLPGTETYLDMLRYNVLQLAHALEQT"
     gene            49104..50288
                     /locus_tag="PK589_05515"
     CDS             49104..50288
                     /locus_tag="PK589_05515"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNJ19088.1"
                     /translation="MKVVGLMSGTSADGIDAALVNIVRRGGRPVITPIAFSTVPYPRA
                     LQQRVVDLSLHGQVAEICHMNAYLGELFAEAALKVIRAAKHQPADIHAIGSHGQTIHH
                     LPKGRRESGVGLIRSTLQIGEPAVIAERTGITTVANFRPRDMAAGGEGAPLVPYAHAV
                     AFSHARRTRLVVNIGGISNVTYLPAGGVMADVRAFDTGPGNMVLDAIMQETTKGNRTY
                     DAGGRLARKGTIHQPLLTELMAHPFLTRRPPKSTGREEFGAPLVRRLLAKQKKARLSI
                     EDLLATCAAWTAEAIGSSRRWVPGKIDDVIVGGGGVSNRAIMASLRTVFAPAPVLTFD
                     ECGWSSKAFEATAFALLAHDVLHGHCTNVPQVTGARHPVLLGSVVPGKADIRRRNRYL
                     PG"
     gene            50298..50822
                     /locus_tag="PK589_05520"
     CDS             50298..50822
                     /locus_tag="PK589_05520"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNJ19089.1"
                     /translation="MNMDVLYPLLAIGALVLLGSLAWETFGRRRAPAASSGSLPSGLT
                     FAPKPLLTDQEVQLYNLLRLAVEDRYLLFTQIPLWSVLDLRVPSGMPPAETLRELALT
                     RATFVLVHPGSRLVEKVVQVEPSASQAEGQGTANSLFDGALRAAGVQLVRLSPFHTYT
                     VPGLVTVLDLADPD"
     gene            complement(50887..51408)
                     /gene="ybgF"
                     /locus_tag="PK589_05525"
     CDS             complement(50887..51408)
                     /gene="ybgF"
                     /locus_tag="PK589_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNJ19090.1"
                     /translation="MTMVQLSREKVRGIAAALALFSILASASDLFAASPQQPDASRRL
                     YDRVMDEFRHKDYPAALAGFRFFLELHGHSSLSANAQYWMGECQYRMGKHDEALGSFF
                     KLISDYPMSQKLAASTLKIGQIYTKQGDLEKAQMMYERVTGEYPDSPEAEVARKAIEA
                     AAAKGDPIAAGPN"
     gene            51717..52550
                     /locus_tag="PK589_05530"
     CDS             51717..52550
                     /locus_tag="PK589_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNJ19091.1"
                     /translation="MDTSHEAGSSGAGKDPLIEPPPNPKGGKTLIVDARDSDAYLCPS
                     AALKDAGEDDQVFVRPGVYEDKVFVADKPVLLVGAGRDQVQIYSRRGGPLYLQRVPNG
                     MISGITFRYVGSDQHSAMNVLDSVCTITRCRAMEGILSGVVIYGPEARPTFIDNEVCS
                     NRESGIFVFAGAQPRVAQNLCYGNHHFGLAVRDPGSHPECVRNVCHSNMLSGMLLFHH
                     AEALLLDNICRDNQHWGLVMTPDSHPTPSQEELVAGNVFLPNPRGSVVVTEQPLADIG
                     R"
     gene            complement(52612..53541)
                     /locus_tag="PK589_05535"
     CDS             complement(52612..53541)
                     /locus_tag="PK589_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HNJ19092.1"
                     /translation="MIKLLLVEDNSVDAQLTQDLLAEWSLDRFEITHASVLADGLTRL
                     SRERFDVVLLDLSLPDTHGLATVTQVLATSPDVPVVVLSGHDDHPLALQALQHGAQDY
                     LVKGEGGTEFLARSILYAIERKKAQERLTYFAQHDPLTGLINRALFRDRLIHAMARSK
                     RKDQPLAVMLLDLDRFKAVNDTLGHDIGDQLLKEVATRLMECVREVDTVARLGGDEFT
                     AILEGISGEDDVLVVAKRIVDSIGSPFQIGPHNISIGVSIGITLYPSDDEDLDELLRH
                     ADKAMYAAKQQGGGRYHFHSPAGHSPDVPAPLP"
     gene            54053..54829
                     /locus_tag="PK589_05540"
     CDS             54053..54829
                     /locus_tag="PK589_05540"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNJ19093.1"
                     /translation="MAQEQSARDQESFSRTSSVDDCLDGKTRTSVSWALPLAIAGVLV
                     VGDSASAENDIYGYVGANGVFEMTNVPTDGRFRPAELQRSRLSHRASVEEVEDAVERY
                     AWQFHLHPALLLAVIKAESDFNPTVISRAGAVGLMQLIPETAIRHGVHNLYDTGDNIR
                     GGARHLRYLLDRFNGNIRLAVAAYNAGERRVERYHAIPPYPETRDYVRKVMIYYKSFR
                     GDYQATPGKAVLLAMHHKRLQLEPLSVPVDFRHGRPAAKK"
     gene            55427..56284
                     /locus_tag="PK589_05545"
     CDS             55427..56284
                     /locus_tag="PK589_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ19094.1"
                     /translation="MPDTPFSESRSGPPSHGHLSLRSLLISALAWPFKTFIRRLIGEV
                     FYQQARVTLLHEHGGVGLDWRWGNSLRRVHPIRRDFGWQSGRPIDRYYTEDHFLPAYQ
                     ADIRGRVLEIGDDRYTRQFGGERVTLSEILNVNPGMGGTTIQADLTDAKQIPSNTYDC
                     AILTFTLQFIFDVPAALRTLGRILRPGGVLLVIVPGISQIARYDMDNWGEFWRFTSLS
                     ARRLFEQVFRPEDITITTYGNVLSSLASLHGLHSTELTTAELDHWDRDYELVIGIRAV
                     KRPAANTSL"
     gene            complement(56423..57127)
                     /locus_tag="PK589_05550"
     CDS             complement(56423..57127)
                     /locus_tag="PK589_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HNJ19095.1"
                     /translation="MHSALTTPTEITASTPRHQCLIYKGSPATHFSGLSAVIRQKLTE
                     NYRCLYFNTSAMIAAMRPYLLAAGIDIPQEVERGRLVLSSNPTHLTDGHFSIDRMLGM
                     LEEGLNQALQDGHAGFWCTGDMTLEFGPERDFSKLLDYEWRLETFLQSHPALSGICQY
                     HADTLPNEVLRQGLLAHPSLYINETLSRLNPHYVDRESFTAATYHDPALDKTIRALCM
                     VPDALILNALPPGYLH"
     gene            57554..57790
                     /locus_tag="PK589_05555"
     CDS             57554..57790
                     /locus_tag="PK589_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HNJ19096.1"
                     /translation="MRQTIKARYHDGVLQPLEPLSLADDAEVQVTVDTDLALGTDEIL
                     RRAAQVYQGLSADEITQVESIALDRQHFFREPAA"
     gene            57790..58206
                     /locus_tag="PK589_05560"
     CDS             57790..58206
                     /locus_tag="PK589_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNJ19097.1"
                     /translation="MPQPAVLLDTDILSELLKQHPLVVQRVRSYLTEHQRLAFSIITR
                     YELLRGLKAKQARTQEAAFTLLCQASLILPITDQVVERAATLYGDLHRQGALLPDADL
                     LIAATALDAQRPLITNNLAHFQRIPGLIVESWKRSV"
     gene            complement(58595..59107)
                     /locus_tag="PK589_05565"
     CDS             complement(58595..59107)
                     /locus_tag="PK589_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19098.1"
                     /translation="MTGMLMAVRNLAFLLLVVLPLSYVDNSDGKIVEEDLEAMVHESK
                     LIVLGKVASAEFTGQILTDEFKIGKWSLFKAVIKPDLVIKGRLQQDELDLYYIGGMST
                     EARFNVNDTSIFFVDEDDQGRLATVRGYAGKVDITQQVAMPVAIRNEENPQPLDAFIR
                     KIRAIVERAR"
     gene            complement(59127..60287)
                     /locus_tag="PK589_05570"
     CDS             complement(59127..60287)
                     /locus_tag="PK589_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNJ19099.1"
                     /translation="MTYTYDKGGNRITQLRQNAAGSNLPTAVAAANIAYDAANELTRW
                     NSATTNLTYNSNGNLATETQGGLTTTYTWDARNRLSGISRTGLTASFAYDALGRRKSK
                     TINGATTGFWYDGNDVYAEIMGATPSATYIRGLSIDEPYIRKGANDEFYETDALGTSI
                     ALTNAAGASQTTYTYQPYGVSTQLDTPSSNAFQYTGRDNDETGLIYYRARYYHPNMQR
                     FVTEDPLRFRGGENNFYVYTNNSPTQASDPLGLFMIDSATCRNKDTRGFIRSMAAVAY
                     DTAMNKPNCLNCSQRQAFKARWEDLVISCWPSNYTPNGRPACGFQNDSLGTNVIWLTP
                     QGLNAQGCWSVPGTIFHETLHTLTSMLGPEYAGEQIVRELEANCFTRPPTMK"
     gene            60797..>61553
                     /locus_tag="PK589_05575"
     CDS             60797..>61553
                     /locus_tag="PK589_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HNJ19100.1"
                     /translation="MDSGFTLTIDWLAFTVLASNPQETMKVLGGDWSKAKGGFRGYPL
                     SWMRADGLRGVGKLGTNAPRRPNEIHVDLSGGLASALTLDQIRTLLQWVHKQQGHVTR
                     IDCALDDRAGTVPVATIREAVSAGQCVTRAAQVRHIVSNLTHGTGATTGETMYFGSSQ
                     SQTLLRIYDKRLELQSKGQENYQDYGTRWELELKKDRAEQCARALATLDEADWKELVI
                     GLLRSYVDFRQIPKDAEDEERYRAPVLEWYALLT"
BASE COUNT        13075 a        16573 c        18446 g        13459 t
ORIGIN      
        1 ttgccaaggc gagggtcgcg ggttcaaatc ccgtttcccg ctccaatttt ctcaaacact
       61 tgcacaacac tccccgacgc tctcaccctc attgtgatag ttttgtgata tctgagggtg
      121 aacgggctcc agaatcaaca ctccttctct taaactctcc ggcgagtgat gcgcgtagcg
      181 ctgcgtcata gctgctgttt tgtgccccaa cagacgttgc accttgtaca aatcgacacc
      241 agcttgtgcg agccgagtgg caaaggtgtg tctcaaatcg tgaaagcgga aatcgctcaa
      301 cgctgccttc gttgccccga gtctgaatgc tcgcgtgagg ttaccaggaa caccgttagc
      361 gctaacgtgc gcgtgggttt tgtggacact tgagcgcgac tcttcgtgct tgcagtcaga
      421 tgcatttacg gacaagtagt cgtgtaagac cgctataaag agactctatg gcaattcgca
      481 agaagactgt gatcggtaag taaactatcg tatgccttcc tgatagagtt gatcctcgag
      541 acatcaaata gtcacggcgt gcaggggcgg ctgagtgcca aacgaatatc gcgctgcgca
      601 gagactcata ccgcttaacc gaacgctctt ttatcatttc cccaaaaatc cagcatgcat
      661 ttgcgcctgc ggccaaggcc gtggacccac cctgccctgc gtctaacagg atcaccgcaa
      721 gtctacccgc cgtcggcaaa gtgggcacgg ttcaggattt cagaccaggc ctttgctgaa
      781 aggcgtgagc cacttgaccc atcgaatggg aaaaggttca tcgcctatcg gtttgaaggc
      841 gttcatcctc tttcaaattt tccggtcttt tgataagcct ttagacatga tttcattggt
      901 atccctacct gttgcccttt gcgggctcgc agcctctttt tttagtttca tcgtgatctt
      961 ctatcgtctg caacaccagg gaattgatct atggcatgca ggaggcctac tttttatcat
     1021 cgctgtcttc tgtctcgcct gtatttcttt gagcgcccac taccgacaca ctcgtcggat
     1081 cgcttctaaa gcttttactc ttcagctgac ggctacgggt attgctttgg cgttgctctt
     1141 tcttggggcc gaggtgatga tacgggtcgt agccgaacag actcccgagg aaacgttcgt
     1201 gggggacact gtcctgttgc caagggaatg gcagcgggtt caggctcacc gacttgcaga
     1261 ttgggaaagg tctcgtcaac aatccacaat aatcgagccg gacgcgacac tcggttggac
     1321 gataggcccg aaccggaagg agatcgggat tttcggggaa atgtacttca gtaacgccta
     1381 tgggctgcga accttggcga acgaaaccga cccgaaagcg accgcccccg ctctccgcat
     1441 cgccatctcg ggaaactcgt atgcctatgg actggacgtc acttacgacg aaacttgggg
     1501 ccatcagctt gagagcagaa ttggtcaaca tgtccaggtc ttgaactttg gagtgcctgc
     1561 ctatggttta gatcagtcct acctccgtta tctgcgagac atccaggatt ggcaccctga
     1621 tatcgtcatt ctagctctga tcagccacga tcttgtacga agcggtctcg tctactatct
     1681 aataggattt caaggtggca gggtgccagg agccaaaccg agactctcag tagtcggaga
     1741 agaacctttc gctctcaatc tcccgttgcc atctcctgag cggatttatt ccacttcatc
     1801 cattgaagac ttgccgttcg tggacttcga tattgcctac cgcccagcgg actggaagtg
     1861 gcatctttat caatattctt acctgctccg attcgccgta tcatggaatc ccacgtctct
     1921 atggtccccc tacgagttgg ccccagacgt tgagagggtg gataaggcga ttctgaagtc
     1981 atttgtcgat acggtccggg caacaggctc gacgccatta gtggtctttt tgcctgaata
     2041 tggtgaattc cgtcattcgg ttcgctcgcc atctgaacag cagcttttag gcaggcgagt
     2101 gctgcaggaa gcaggggtga aatttcttga tctgacggct tgtttggagc ttgtaaatgc
     2161 gactagtcga tttaccctcg gctggcatta tactgcagcg gctaacagcg ctgtggcgga
     2221 atgccttttg gacgaagtgt cgagacttgt tggagtgatt ctcgagcaca aatcttaggt
     2281 gtaactggcc ggcaagctgg ggcacggttc aggtcacggt gtgtgacacg ttttggtcgc
     2341 gatacctcag ttgtctaggg atagcgacat tacaaactaa attggagaac gagccgtctg
     2401 gtctatgatc atcaaccttc tgtcactacg ggtctctttc catactaaca actcaggaag
     2461 ccttgccaag gggagggtcg cgggttcaaa tcccaaatcc cgtttcccgc tccaattttt
     2521 caattatctc cacgcaagat cgttccagat ctccttgtca ttgatcgttc ctcgtgcacg
     2581 tgcaccggtt tgggcattac atgcggcaat gcacgcccct aagtagggac agatagctag
     2641 ccctggcggg actcggcaga gtgcaaaccg tataagacat ccgtgcggag tacgtacttc
     2701 agtccgctct gcaccacggc accttcgtgc caaatggcat gcagaaatac aagcgccatt
     2761 cccatcttcg gccgaaccga cagatacgga gcaggaggtt gcaggaatgc atggtcggcg
     2821 ctcgcataga acttcgtctc cccgcccacc atcccatcat tgagatagat catcatcgtc
     2881 acttcacttt tgtctccggt cttcatccgc agataggagc cgtcgcgatg aggctggaac
     2941 gtctggccgg gttgataccg ataaaatcgc cgccgttcgt tgaagccgac gagatgatgg
     3001 ccatcaagaa cggctgggag gaagggttga gcgcgttgaa ataagtttga cgcgagggac
     3061 tcatcatcga cgatcacccg ctcgttattg cggaagtttt cattcaccac gccaccgacg
     3121 gttccaggtt cataggccgc tgcttcactg cgccggatca gtgccgcaca ctcttcctcc
     3181 gaaagaaaat ggtcaatgac gaagactcgt tcggcatcga gcagttcttt aatcacaacg
     3241 acttccttcc gtttcgcctg gcgtgtggaa gtccggtggt ttcacgtagc actagcttga
     3301 tggattgagg cgtgagtgcc ctggttactg cgagtcggcg gtcttgcgtt tgagaagtcg
     3361 gagtgtgcgc agctggactt ctgccgtcgc gaggtctgcc tcagtcagcg ccgcttctcc
     3421 gaatcgtact cgagcataca gccgcgtgac ggagtcaacg agaggccaag cctcgcccca
     3481 tcgcgtccga atctgttcga gaaattctac tgaggttgcg ctggccggtt tcgtgattcc
     3541 ctgctgcgcg cagcattgca gcatggtggt gtagagcgcg atgacggtct gttgctgcgt
     3601 tgaaaatgga ctgcgtggcg tggtcctcgt ccggaacctc cgcaagatca ctatccctcc
     3661 atagactgcg gcggctgcca gcacaagagc gatcacaacg tgcggaacac ccgagggagt
     3721 caagcggatc accatatgac tcacgatggc gacaccctgc gctgacaggg cgctcaacgt
     3781 ctcggaaaat cctgcccgca cggcttctcc gccttctcga atgccttgca ccacggtgaa
     3841 ttgatcatgt gcgctgtagt ggacgaacag gcgatcccat tggaggcgaa tcgaatccat
     3901 cgcactgttg gccgattgcc accaggtcgg accgactgca ttcggcgagg gcggggtggg
     3961 atccatggtg atccagcctg attgggggaa atacacttct acccaggcat gcgcatctcg
     4021 ttgacgtacg gtgtagtacc cgccgaattc attccattca gtggcgagaa atccggtcac
     4081 caggcgtgcg gggatgccta cggagcgaag catcaccacc atggccgtcg catagtgctc
     4141 gcaatagccg gtttttcgag tcagcagaaa gtcttcgagc ggatgcggag actgcagcga
     4201 ggggacatcc aggttgtatc ggtaatgagt cagcaaatgc gtgtgcacca tggcgacggc
     4261 ttgggcgacg ctggttgcag gacgcgtgac ctggcgcgcg agttcgagga cctgctcatt
     4321 caccgcgggc acctggagat attgttgcag aatagagtcg gggtagatca acgccgcgga
     4381 ttttttctcc gcggcattca agctggtcgg tgaggaatag acggtgtatt ggctccgtgt
     4441 gtggaggggg aacggcagat acagggagcc catcagatcc gattggaggg agaggaagtg
     4501 gcctttgacc gccgtgggaa acgggctgcc gaagaggacc gtggtgtcga gtggctcaag
     4561 aaggatctcc agtttgagct ggcgagccgg gggtgatggt ttggaggcta acgtacggag
     4621 cgtgaaggtg ccttcgggaa gctccgtgag catgcggcgg tgcggcaggc tgttgctcca
     4681 ggattttcca tcataccgat tgtaggcgac gcctcgaagg tacagtggct cccacttcgc
     4741 gtcgtgacct gtgcgttcag ggagttccac tcgcatgaca atgctggggt cctgcttgac
     4801 gggccccatc actcccaagt cgacatgctc cgaaaaccct gtcgtgcgca ggttttctcc
     4861 atgattgttt tgaaaaaacc ctactcccac tcgcgggatg agaaaaaaga acagcaacgt
     4921 gagaccaaac gcgctcgccg ccatggcgtt ggtggtccag aaaaaccggg ccgtaaggcg
     4981 gggaaggggc acgggtgacg gctcggcgtg agcgtgccct ggttccggtt cggtcagctc
     5041 ttcccgttcc tgcagcaggt ggtagagcag aagcgtccac acgccgatga ccaggtagat
     5101 gaagaaaaac ggggcatacc agatttgcgt cgtcagcgcc gcggaggcca ggagtgcgat
     5161 caggctgatg gcgtagagat gcaggaagtc gcgtcgctga tcgagattcg agagtttgtt
     5221 gacgaggagc aggacaagaa agtgaatgcc tgccggaagg atctcctgtg agatgaggaa
     5281 gaggtcgatc cagaatccgg cgaacgcaag caggagaaag atattccacg tgagcgctga
     5341 gaaccgcaga tgcccgatgg caccggagat cgcgcctgaa gactggacgc gcagcaatgc
     5401 catgccgaag gctcccgccg ccaggactaa gagccacaac ggcaaggcca tggcgagtgt
     5461 caggctggcg aagccggtcc cagccaggag aatggagcta agccggaagg cctgatccat
     5521 cggcatcgaa gaggtccttg tgcgtgtgag gggtaagaat ttgatccaca cctggaaaca
     5581 gatcctggag ggccgggtct gcccatgatg ctatcaagag cgtggggcca tcctgggacc
     5641 gtacgagagt tgagatcagc gcctgctgga cggctgtact gtcgtctacg ggccttcgct
     5701 cacagagtgc cagggcatgg aacagatgaa gcggtgactc gtttccctcg gccatgactt
     5761 cttgttgatc gcccagcagc aggcgaaggc gaaccccgct cttcagcaat tgatcaatca
     5821 aagaagcggc gatagagagc gcccgttcga atgttgtcac ttctgcgtcc gctgccaccg
     5881 taaagaccgc aagcgtgatg atgcgttggt cttcggcttc agtctctttg agcatgagtt
     5941 tggccgttcg tgcggtcgtc atccaatgga tggctcgcga atcgtcgccc gggcgaaact
     6001 cccgcagatt gtagagtgaa tttccgtggc cacgtcgagc caaggcgtga tcatggccga
     6061 tcgcgtgtag tgcctggacc tgcatgggtg gaagcggaat gagtgccggg cagacgatga
     6121 cgctggcctc cagcggatag aacgctttct tatgaaacag cccgaaggga aacggtgtga
     6181 cgactcggat tccgtccaat tgacagcggc cacgccgttt gatgagcagc gggtacgcac
     6241 gcaacgtcga cgcgcccggc gcaaggtgag tcagatggat cccgcactca tgatcctggc
     6301 cggcggcgac gtcgaggaag cgcaaggaga tgctcggaag atgggatttg cgattggcaa
     6361 tccacaacgt tgcggtggcc tgctgattga caaacaacgt gtccggcaga tggcggtgga
     6421 actcgagtcg ccgcacgcat tgttccgaca agaagccgga taacacgatg aggctgagca
     6481 tcatcgccag caacaggtag aacagattgt tgcccgtatt gacggcagcg ataccaaccg
     6541 ccaaggtaag cagcagaaag cgtacgcctt ccgttgtgac gctgatcgcg cggtgtctgg
     6601 agagctggtg aagccaggta cggagagtta tgaaagccat gctggtggcc cgtcatccca
     6661 tgagcggccg ctggcggccg ttatccgata gcctgcttcg tgtgcagggt ggtcacacgg
     6721 gaaccgggat gctctccaga atgtcgtgaa taacccgttc ggtgtgctcg acactcttcg
     6781 tgcgtgcgcc ctgagatcgg ttgagcataa cgcggtgcga gaggacggcc ggagcgagtt
     6841 ctttaatgtc ttccggcaag caataggttc ggtctcgcgc aagggcgagg gccttcgccg
     6901 ctttgctgag cgcgagcgct ccgcgcgtac tgacgcccag tgaaaataag gggttttgcc
     6961 tggtggcaag cacgatagcc agcagataat ccatcaggct gtcttccatt cggactttat
     7021 cgacctgggc ttgaagatcc agaatatgct gcgcgttgag aaccggctcg atttcgttcg
     7081 ccggatgcag cgatggcggt cggtccagca ccttcttttc ctcttctggt gtcgggtatc
     7141 cgatacacag ccgcataagg aaccggtcca attgcgattc cggaagtgga aatgtgccgt
     7201 gatactccgc cggattttga gtcgcaatga ccatgaacgg ctgttgaaga ggataggtct
     7261 gattgtcgac agaaatctgg gcctcgctca tcgcttcgag caaactgctt tgtgtttttg
     7321 gcgtggtgcg attgatctca tcggccagga cgatgtgggc gaagagcggt cccggcatga
     7381 actcaaaggc ctgtttctga cgattgaaga ccgatattcc gacgatgtcg gaaggcagca
     7441 ggtcactcgt gaactggatg cgcttgaagg agcagtccag ggaacgggct aaactgtggg
     7501 ccagggtcgt tttcccgacg ccgggcacgt cctcgatcag aagatgcccc cgtgccaaga
     7561 gacagacgac cgccagttca atcgcccgcg ccttcccctt gatgacctgc gcgatattgt
     7621 cctgtaacag tttgatagat tgagaagaat tcacgatggg tttgtttgtg aagggggtta
     7681 tgttgggcac atggcgtgta tgcccgaagt ctaacaaagg gtctggagac cgtcaattta
     7741 tacggccttt catgcggggt ggctcggtga tcgatttgtg aggcgccaga gcgggaacca
     7801 ggcgagtatc aggaaatggc gacgggctcg ggtacgagcg tcagcaacca ggggggcgtt
     7861 tcctcagaca tgccttggaa ccgcctcgct gtgctactgg cagggagtcc tgcgaatagc
     7921 gcaccgcgct gaattttgac ctgccggaat cgataggggg caggatgacc gggagaggtg
     7981 tgcagaacag acggaaaact cttccgcagc ggcgtcaggt ccgatagcag gcaggagcca
     8041 cccaccggca gagctgagag ttggcggtag gtttccccga agatcgctgg cagcgtttct
     8101 gccgacaaca cgacatcggc gggtctgaca cgcgggtcat cgatcagttg cgagagaatt
     8161 tcccaacaca cctgcgagtc caccgccccc aacacacaga gggctgcccg gctggccatc
     8221 aattccagca gggcgagcgg cccacgaata tcgaacttcc acggtggaaa cagtaagttt
     8281 cgtccggcat ggctcacctc gagggccggt tctttgccat gtcgaatctg gcgggaatct
     8341 cccttggtgc gtcggagctc ttcggcgagg cagcgggcag agaggcgagt tggttcatag
     8401 gcaaaggtcg gcgtagccgg ggcccaacag gtgacgatga gatatcgggc ggccaagcgc
     8461 tgatcccgtt ccaaagagtc gagggcgaac atgtcctgat cgccatagaa gcggaactcc
     8521 agaccgggct tgctgcgcag gcgattcagt cgaagcggag gggcatgtaa tggtcctccg
     8581 agttgggagg aaggatccag ccggtcgagg cagtggagta ggagtggtct gtgttgcgcg
     8641 gtcaacgggg ggtcatagat ggtggccagt tcttccgcaa acgaaagatc ccctgatccc
     8701 aggtctaaca acgaggccgg aagttgctcc agtaacacat cgaatggatt gtgatgcgtc
     8761 tggacgaatc tgtcgatcgt gtctggcgtg acggtcggca ggggccattt ggccatcgct
     8821 ggtcgtccga cctgaaagaa cacgcataag gcccggagag cttttgtgag gggaagcccc
     8881 gtgagctgct tgaggagcac agcggaagaa gtgcgatctt cagtcgcgag atccactggt
     8941 aacgccgcca ccagagtctg cttcagtccg tctggtatgg aagaagcgtg gatcgccttg
     9001 acgagggctg tcctggttgc gggccagtct gccgaatgta cgaggtggcg cgaggcctcc
     9061 cataacggcg catcgagcga tctggcggat gtgacctgtt tccggaatgc ggccaggctg
     9121 tcggaatcaa caggcatcag gagtcaacga ccggcgtgtg ggttgtgggt gatcgaccgt
     9181 tccgttgcgt cgcgcagagt atcgggccgg ttgtaaagga gtcaagcagg tcgtcttgcg
     9241 gctgcatgat gcgtatgtta ggtagacgat atgcgtacat cattacagca tttacgtgag
     9301 aaggtcttga acaacctcag cggtgtcatc gcgatattgg ttttggtggt cgctctggga
     9361 ggctgccagg cggccgggcc tgtgccccgg gtaaccccgc aagtctggga cgcatggtct
     9421 gtcggtcaga tactggatgc caagacgggc cacgtcgttt caacggccga gtggttggaa
     9481 gggttggccg gatatgacgt gatctacttg ggtgaggagc atcacaatcg atcccacatc
     9541 gagtccgcgc tgacggtgct gcggacgctt gtgacccgcg ggcgtcggcc tgtgctcgcg
     9601 atggagatgt tcggctggga aggccagccc gctctggatg cctatctcga gacgaaggaa
     9661 tccgctcgcc cggaatttct cgagcgtgtg ggatggaaac aaaactgggg tggcgctttt
     9721 gaggattatg aaccgctcgt gcagtttgcc aaggatcagc ggctcccggt gctggccatg
     9781 aatcctccca aaacgctgat tcgtcaggtc gtcaagctgg gactggccca agccaaagag
     9841 cagcccgaat ggcgtcaacg gggcatggag caggagacga tagtcgatga cccggcctat
     9901 cgctcccgta ttctatctca actccaggcc tgccacggag ggggggcacc ggaagactat
     9961 cagaccatgt acgacgcctc gatggtgagg gacgagggca tggcgaagac cgtcgcggcg
    10021 gcggtgaatc gtgttcgcgc tgcgggcgac ccgacacagg ggccggtggt gagttatacc
    10081 ggcggcggac acgtccaata tcggttgccg gtaccgaatc gcgtcgctcg gcgtgttgag
    10141 cacggactca agcaggtgac catctatatg gccacgttgg agcaggaccg ggccgcagag
    10201 ctctatcaat ccatgcagga aggtattgca gattttgtgt ggttgacccc gcctgggtct
    10261 caaggggctc ctcgccgatg ccgataagat atcgggcggt cgagtcctcg cggccctgcc
    10321 gtattctgtc gacccctgca gggggctccg tcaccggaat ggtcggatcg gggaacctgc
    10381 cgtacactcg ggtcccatgc ctggatgatg ggcctcaggg gtaagcattc catgacactg
    10441 cggcgatcat tcgataagtt gcaagggcga ctcactggaa ccaaccattc cacaggtggc
    10501 acgatgcggg ggaaaatcgc atgttcggcg gatcgcctca gtttactgga ggcggacatg
    10561 atcagctcca tcgtggagcc caatgcgttt caaatcggaa ggcaatacca gacggaacgc
    10621 cgggtgcagc tgaccgacgc ctcggatacc gagttcacct cctctgtgat gggcaattcc
    10681 gggctgtacg agcagaccat ccgtctccat aacgggcacc tggaggccaa gtgctcctgt
    10741 accctccacg agcaaccgat ctgccggcac ggtgttgctg cgctgctcga atatcaacga
    10801 tggtcgaagg ctcgcgtggt ccccagggcg cgacctgcag ctccccgtct tgaagccgaa
    10861 cgggcaacca cgaaatccgc tccctccggg gatgtgaaac tcagcgagtt gaatcagttc
    10921 agcgactgga tgcaacgagt ggtgcaggct attcaatccg gtcagccggt tcctgcacag
    10981 cccgatatcg agccgggcct ggtctcgacc tggacccgga tcattcagca attggatgaa
    11041 cgacggcggg aaagcgaagt cgcgcaaatt gaactcgata cggatattcg caatcgtgaa
    11101 gcggtcgtcg cccgcctgac tcaagatttg gaagcctccg caaaggaagc caaatcctta
    11161 caggtgattt gccgggatct ccagcgggag gtggatcagc ataaggcctc cctgtctaaa
    11221 tccacggagt tgtcacgaca cgtcgaacaa ttcgagggcg aagtgcgggc catcgccggt
    11281 cttttgacag agaaaggccg gcgtttggaa ggcctctccg atacctgtcg agatgtggcc
    11341 acgatgttga aagccctggg gaaggtgtag cggctacttc cgccttcaaa ccggccctgt
    11401 ctcttccaat tccgaatcga atccttcttt ctgtcccccc ttgcgtcatc cccagtccgt
    11461 tcagtacaat gcgggcctct ttggggattc cttgactaca agggggagat catgaacaag
    11521 ctgatagcag gagttttggg tggaatcggg gcgctccagg cgaccaatgc cttggccaac
    11581 gtcagtgaag ggccaccgga ttacagtggc atcacgggcc tgtactacgt cctgattgct
    11641 ctcatcttgg cgttcggggt ttacgacacc ttcttcaaga agagctaacc agctcctcag
    11701 gatcaagtgg cgtgaaacgt ttctccgttg cggaaggcgg aggggatgtt tcacgccttc
    11761 tttttgctag tcggggaatg ggactcgccg gatttcagca gcggaagcag ggtttgcaac
    11821 acgttctgaa cgacaatccg ataaccttcc ccggtgggat gaatgccgtc ggcttggttg
    11881 agcttctggt cgccgcccac tccttccaga agaaacggaa tcagtgcaag cgcatgggcc
    11941 ttggctaggt cccgatacat cgcttcaaat tgtgtggtgt actcctcgcc gtagttcggt
    12001 ggcagcttca tgccggccag aatcacctgt acccgcgcct ctttcaatcg tttaataatc
    12061 gtatccaggt gcgaccgagt ttccggcagg ctcaacccgc gcaatccatc attcccccct
    12121 aattcgagaa tcacgacgga cggttttccg gcgaggagcc aggatactcg acgcagtcct
    12181 cctgcggtcg tgtctccgct cacccccgca ttgagcacct gatagtgaag gccgagtgta
    12241 tccagctgtt tctgcagctg cgccggatac gtgtgttcgg gggagacgcc caggccggca
    12301 gtcaaactat tgccgaatgc cacgattctc ggtcggctgt cggcctctgg caccgtcgtc
    12361 gtagccggcg ggcccgctga aggcttgtcg tcagtcgccg gctgaggtgg ggcaaggctg
    12421 gcaggcgcgg atgatgaggg aggactagag gccaggggag attgatcgca gccgacgagc
    12481 ccgctcacga gcaggacgat aaggctgcct gtcatcagtc gcgggatgat gcggaaaagg
    12541 tccgagcagg tcgtcatgcg tacagtataa tatgaaaact gtttttgcat gtggagtcta
    12601 cggatcaaca ggatgatgat tgccactcaa caggtgacga tgcaattaga ggccgggggc
    12661 gagacggtca ggattctgga tgatatcacc cttgagattc cagagaagca aaccgtggcg
    12721 attgtcgggc cgtccggcag tggcaagtcc actctgctgg gcctgattgc ggggcttgac
    12781 cggccgacgt ccggaaccat ctggctcaat ggccgggaga ttacccgact gaacgaaaag
    12841 gcgatggcgc gcctgcgcct ggccaacgtg ggatatatct ttcagtcctt ccatctcatt
    12901 ccgacgttaa ccgctctgga gaacgtgtcg attccgttgg agctcgcagg cgaccgtcat
    12961 gcacgccaac gcgccatgga attgctggag gcggtaggct tagggcatcg ggtctcgcac
    13021 tatccggttc agctttccgg tggggaacag cagcgggtcg cagtggcgcg tgcgtttgcc
    13081 tgccggccgc cgattctgtt ggccgacgaa ccgaccggaa acttagactc ccatacgggg
    13141 cagcaggtga tccagctcat catggcgttg caccgggacg ccggcaccac cctcgtgtta
    13201 gtcacacacg atcaacaact agccgcttcg atggagcggg ttattacctt gcgagacggc
    13261 cggatcgggt ctgatcaatt catggcatct tcctacgacg agtccggcga ccgcctatga
    13321 ggccattctg gttcatcctg gcctggcggg acttgcgatc ttcctggcgg cattttgtct
    13381 attttctcgc ctgtatcgca ttaggcgtcg gggcggtagt gggggtgtct ctcttttcgg
    13441 ccaacgtcga acgcgcggtg ctgaaagagg cacggggcct gctgggtggt gatctggaaa
    13501 tccggctatc caggccttta gggggaccgg gctccaccgt gctcaaggac ctgacgggcc
    13561 gcggaattga ggtcacgcgt gtagatgagc tggtcgccat ggtggcgcgg gtcgaccgtc
    13621 gcgaaggcgg aatggacgtc acgcagctcg tcgaattgaa agccgtcgag ccgcgatatc
    13681 ctctgtatgg cgtcgtgcgg gttgagccgg atcgaccatt gaccgaattg cttcatccgg
    13741 ccggtgaccg ttgcggaacc atctgtcacg gggcggtcgt gcagcaagca ttgttgatcc
    13801 ggctgggctt aggcgttggt gatgcgatga agattggaca ggcttctttc cggatcacgg
    13861 gcgtgattca tacggagccg gatcgcatgg ccaacatgtt cagcctcggc ccgcgggtgc
    13921 tgatttcgca agaagggctg gccgccgccg atctgatcaa gccgggcagc cgaatgcgtg
    13981 aacggcattt gttacggctt cctgcctcta tggcactgtc cccgctcctt catgagctgc
    14041 ggggccgcct tggcgtcgaa tccgcgcggg tgtcttctta tcgcgatgcc cagccacagc
    14101 tcaagcagtt tctcgatcaa ctcgcgcggt atttaggctt ggtcgggctg acggcgctgt
    14161 tcgtcggcgg catcggcgtg gcgctatcca ttcatgcgtt cgtgcgagag aagctcccct
    14221 ccattgcgat tttgaaaacc gtgggggcgg ataccgcaac cataatctat tcctacttag
    14281 ggcaggccgt cggcttaggc ctgttgggta gcctgggagg gatcggcatc ggcgtggcct
    14341 tgcaaacagt ccttccgcag gcggtgtcca cgttgttggc gacggatgtc ctccagcaag
    14401 tcgagtttac atcggtgctc tctctttcag ccgtggcccc tatcgggaaa ggcttgggtt
    14461 tgggcatcct gacgacgctg ttgttcagcg tgtggccgct gctgactatc cgagatatca
    14521 aacccgctgc cattttcagg cgtgaagtgg aacgtgttgg tgcgccactg gtgcgacgag
    14581 agaaagcctt gtggaaacgc gcgggacaag tgatgacggc ggatcccctg cgaaccgtca
    14641 cggcggcggg tatcgggatc gggctggcga gcttgtctat gtggcaggcg ggatcgctga
    14701 cgatcggcgc actgtttatc ggtggcttgg tgatcgcgat agcggtcttg accttgggag
    14761 cccaggcatt gttgcgggga ctccgcgcgt tgcccgtacc gcgggagttg tctttgcggc
    14821 aggcgctggg caacattcaa cgacccgggg ggcaaaccct aggcgtgatg gtgtcgatcg
    14881 gcgtcggcgt gatggtgatc ctggcgatcg cgttgcttca gcatgcgctc gtgcggcagg
    14941 tgggagaaaa ccgtccgtcc gattctccca ccttcttttt catcgatatt caaccggatc
    15001 aggcacaggc cttcggggag ttagtccatc ggcgcaccgg cgattttgct cccgatctga
    15061 ctccattggt gcgctcacga atccatgcca ttaatgggcg gccggtgact gacgagcgcg
    15121 aggctcagca ggatgaaccg cctgctcagt cgaaggaaga gaagcgcaaa agctggtatg
    15181 tgactcgtga gtatgtgctg acgtttctcg atcaagtgcc caaggataat aagattgtga
    15241 aaggcgcctg gtggaaaccg gggcaggtgt tttcgaaacc gcaggtgtct gtcgaggaag
    15301 aagccgccaa gagtctcgat cttgatgtcg gcagcctgct ggatctcaac attcaaggaa
    15361 ccattctaca agcggaagtg agcagcatca ggaaggtcga gtgggggaac ttttccacga
    15421 atttctacat gatcttttcg cccggctcgc tcgatgcagc gccgatgaca tacgtcgcca
    15481 ccgtgcgagt ggctccccag gacgaagtgg ccttccagtc ggccgtggtg gctgcgtttc
    15541 ccaatgtgac cgccattaac atcggcgaag tattgaacag ctttgcccgt gtgctcgacc
    15601 gcttgtcgtt ggcgattcgc ggcgtcgcgc tcttttgcct gatggctggc gcgctggtga
    15661 tggcggcggc cctcgcggcg acgcgctatc ggcggctgta tgaggcggtg atcctcaagg
    15721 cgcttggcgc aacccgcggg ttgattgccc ggtcatttgc agctgaatat gcgttgctcg
    15781 gatgtgtggc gggaatgatc ggcgtcgccc ttgccagtct gttctcatgg gcgatcttac
    15841 gatatatcct ggaactgccg tgggcgttgg agctgcccgt gctgggcatc ggattgggct
    15901 gtaccgttct gctgacgctc ctggtcggat tcctcagtac ctaccgcatt ctaggccagc
    15961 cccccctgac cgtgcttcgg cacgaatgat catggtgcga tagaagctcc gattgaatca
    16021 ggtcgaccta cgcgctaaca cggcgcgaat cgcttcgcgc tcacgctcgc gaaattgcac
    16081 ggcgatatga agttgttcca gattctgttc cagcgtcttg ccgccgagat cgattttgcg
    16141 ggacgtaaag aagtgccgga cgtcctgttc cagttcaggg gttgagagac cggtgaggcc
    16201 cccgcacatt ctccgcaaac cgctcttcgg aaacagcttg tccattcgct cccaattgct
    16261 cttcacaaat ttccaggcct tctcgcggat atacacgttc tgaagcaggc tgctgacgag
    16321 gaatggcgca tcttgcgtac ggatctcgtc ggtcagggtt tttgttaacg tccgttccag
    16381 cagctccggt cgacggaacg cggccagaga aaagagatag cgacgttcct cttgtggagt
    16441 ggtggcttgg cggaaccggc ctgaaaattc ttcgtagcga gcctcatccc cggtgaaggc
    16501 cagaatcgaa acgagtgctg gaatcacatt gggatcgatg ggccgggact tctgctgaat
    16561 cgcggtgtag gcctcccgcg cttgagcctg aatggtttcg tcacgtccca aggtgcctaa
    16621 cgcgcgaatg atatcaccgc gcaattcctt gacgagatcg cgctcatcgg catggggcga
    16681 ccagccgagc tgctgaaaca ggggcgcaac ccgatcgcgt accaacgctg cgagcaaggg
    16741 acgatcctct tcttccagga gatggttgat cgtggaaaat gaacccagca tgacagccca
    16801 gacatgcgga tcctgttcac tgcggaaatg ttcagtcaag gcgaggtagt cggcggagga
    16861 gaccatgccg gcgacggtgg cggcccaggt atcattcaac aggttgaatc gttccaccgc
    16921 agtcagggtg ttcagaccat ccttctgcaa ggcactgagc aagtcggcgc tgtatcgcac
    16981 gcggtaaaat ccatgccccc cttcattggc caggatcgac gtccagccct ctggaagagg
    17041 aatgcgattc tctcgctcac tgagcaatac gcgtctggtt tctgtgccgc gcgcggtgtt
    17101 gatccgcagt tgaaccggca cctgccaacg tggcgacgca gtgccggagg gtgccgtgga
    17161 ggaatcgtcc gcgtacatga accggcgttg cgtgagcatc aacgtggagc gtgactcgac
    17221 ggagagggac aacaagggat acccgggcga gaaaatccat tcgttcatga gcgccgggac
    17281 atcctgtttc gaggcgtggc caagggagac ccagaggtcg gtggtctcgg cattcccata
    17341 ggcatgtgca tgaaggtagt ggcgtacgcc gtcccggaac actgtcggac cgatatgttg
    17401 ttccagcatg cgaaggaccg aggcgccctt ttcatacgtc aacacgtcga acatggcttc
    17461 ggcttccttc ggcgcgcgca cgggaaattc aatcggcctc gtgctcagca ggccatccac
    17521 cgataatgcg gcggcgcgtg ccatgccgaa ggctgtccac cgctcccact ccggtttcca
    17581 ggcatcgact accagcattt ccataaacgt ggcgaaggct tcgttcagcc agagcccgtt
    17641 ccaccaggcc atggtgacca agtcaccgaa ccacatgtgg gcgttttcat gggccacgac
    17701 gtccgcaatg cgcccttgct ccgcatgtgt ggcggtgcgc tgatccaata acaaggcggt
    17761 ttcacggaag gtgatggcac ccagattttc catcgcgccg gaagcgaaat ccggaatggc
    17821 gatcagatcg agtttgtcac ctgggtaggg aatatcgtag tactcggcta gaaagttgag
    17881 tgagtagacg gcgatctcgt gcccgaatgg cgtcaagtgc tgtttgcctg gaacggacca
    17941 tagtcggacc ggggtctgtt tggccatgat cggcgcggtc gacaccagtt cgcccacgat
    18001 gaatgccacc aggtaggtgg acatcttcat ggattcggcg aatcgaacga tgcgtttgcc
    18061 gttctccagc cgttcgtcca cgattctggt attcgagatg gccgtcaacg ccggatcgat
    18121 ggctaaggtt acggcgaaca cggccttgaa gtgtggctcg tcccaacagg ggaaggcccg
    18181 ccgcgcgtcc gtcgcttcaa actgcgtggc ggcgaggcta tgtgcgatac ctcgatcgtc
    18241 tttgtagctg ctgcgataga aaccccggag cttgtcgttc aacgttccgc gaaatgcgag
    18301 gctcaatttc cacacacccg gtggaatgac cgctgggaag gtgatgtggc atcgctgatg
    18361 ttcatcatcc atccggacgg tcccggtgcg tggtggctgt ccctcgccgg acaatgtcgc
    18421 tgccgacacc tcgagctccg tcgcatttaa gagaatgtca gtcgtgggtt cagtgaccgt
    18481 cagcgtgacg atctcatgcc ccgtgaacga atgtgagggg agatccggct ccatccgcag
    18541 gtcatagtat gtcggaatga catggcgcgg gagacggtag ggatcagcaa ttgcggatga
    18601 agtcgtcata gtgttgtgag gatgttgttg gttgtgcgtc gacgttgttt ggctccgggc
    18661 ctcaggcacg aagaccaggg atgcgattcc cggccatgcg cccagccaca gggccgcatg
    18721 ggtagcggca cgtacggcca gagcagccgc ctgccggcgg gttaccggca ggtcgccctg
    18781 cggcctggtc atggcttcgg agcatgggtg acgtcggtgc cttgtccatg accgacgatc
    18841 aggatgctgg ttcgtccgat gaacaggccg gtttcaacga tgcccggaat ctgattgagt
    18901 tcaacttcca gcgcagcagg gtcatcgatg atgggcatgt gcagatcgag gatgacatgt
    18961 cctgcctcag tctgaaacac gtctccgtta cgttggcgca agaccgctct gccgcccgag
    19021 accgcttcga tgtgtcgggc ggtgctgccc cacccaaacg gcaccacttc aatgggaaga
    19081 gggaaactgc cgccgagcgc cggcaccagt ttcgtgtggt caaccatgac gatgaatcgc
    19141 ttcgccgcgg cggcgacaat tttttccttc agcagcgcgc cccctccgcc cttcacgagg
    19201 ttcaatgcag ggtcgacttg gtcggctccg tcgatcgcga catcgatcat ccaggcattg
    19261 tcggattcga taagcggaat gccggattgc ttcgcgaggg ctgcggtgtc gtgcgatgtc
    19321 gggacggctt gaatattcag ccctgcacgt acgcgctcgc ccagagcgat aatgagatgt
    19381 ttcgacgtgg tgccggttcc gagcccgacg accatgccat cgtggacaaa ttccgttgcc
    19441 ttgagcgcgg cttggcgctt ttccgaatcc aggtcacagg atgttgttgt cataatgaag
    19501 gaggaagtgt gagttgtgcc gccacggagg cagcgccgag cagggcggtt ttatcgttca
    19561 tcacgacttt caccggtatt tggttcatca gtcgtttgta gcggcctttg ttggtgaagc
    19621 cgcgcatgaa tgagccgtcc tgcagtttct tcaggagttt gggagcaatg ccgccggcga
    19681 catagactcc gtccagcgtg agggctttca gcgccaggtt gccagcctcg gccccgtaga
    19741 cggatgcgaa taattccagc gcttgtgtcg cgatttctgc ttggcctttg agtccggctt
    19801 cggcgatctc ggctgccgga ttgccgacct tgatcttctc ggccagccac gttggctcat
    19861 tcttcttcgt gtcgcggaga tactcgtaaa tcgcatggag gccggggcct gacacaatac
    19921 gttcgtagct gacgtggaga tagctgccgc gaagatgccg aagcaattcg atctcgctgt
    19981 cgctattggg tgcaaagtcc gtatgtccgc cttccgatgg catagggcga tagcgggttc
    20041 cgtcccaata gaggatgcat tcaccgagcc cagttccagc cgcgatgagg gcgagcgcct
    20101 gttttttctt gggcggcgcc cccgcattca gcacgaccat ttcatcggtc gtcagatgca
    20161 gaagtccatg cgcggtggct tcgagatcat tcaagaggcg cacgtggggg atgacgaatc
    20221 gttcgcctaa ggcggctcca tcaatgaacc agggcaaatt ggttgtgcgg caacgattgt
    20281 cgatgaccgg tcccgccacg ccaaaacagg ctgccgtgag cgtgatgggt tctggaacaa
    20341 ctggggcggt ctcagcggcg acatcactgc caaggtcgac ggattcctcc gtaagagcgt
    20401 tctcgagcgc cggtttgacg agcggggtgc tgaggaactc gtcgatgatc tcttcaaggg
    20461 atttataatc cgcgctgtga aaactgtcct cgcgcaccgg ttcgacccgc ccgctggtcc
    20521 actcatagag ggccaagttc gtctttgtcc cgccgatatc acctgccaga atcatggttc
    20581 cccttatgat gacaactcat ctgtcgcctc agtcaatgcc gatgcggcgg cgcggtcaaa
    20641 ataccataac agtcgccctg tctcgggacg gatccttgcc gccggatagg catcgggggc
    20701 gtcggcacga ctttcgaaaa tacgccggac aacggtggcc ttattcagtc cggtcacgag
    20761 gaacagtacc acactcgcat gattgatcac acctagggtg agggtgatgc gggatcgcgt
    20821 gccttgcggg gctaggccgg gaacgaccca gcgagttggt tcgctgagtg ccggcgtacc
    20881 tggaaagagc gaggcggtgt gtccgtcttc tcccatgcct aagaggacca ggtccagtac
    20941 aggccattgt tctgacgttg tggccgtcag gccccgcaac aggttttcat agtgaatggc
    21001 cgcggtttca ggcgagtcct ctccgcgcat ccgatggatg tgcgtggccg aaatattcag
    21061 tggtgtgaac aagagagtat tagccaaggc aaagttacta tcctgatgtg taggcggaac
    21121 tgcgcgttcg tccccgaaca ggaagtgcac cttgttccag tcgagctgtg tggcgaattc
    21181 tttactcgcc aggaccatat agagtgcctt aggcgtggaa cccccggata gagccaccag
    21241 aaagcgtgca cgcttgctta tggcctgttg gccaacagtg acgaagagtg ttgctgcggc
    21301 acgaacgacc tcttgcgcat cagcaaagat gtaccgttcg ggagggtgtg ccatctcaac
    21361 ggcgggatcg acgagttgac gtggttcgct tctttggcac ggatttccgg cgcgtggcct
    21421 tggcgggtgt aaggaaagtg acgaggctgc ggatggtccg tacgggattg gggccgagag
    21481 cgatgcggac ggccgggcgc tggtgtgtgt gtaacgattg cagatcaccg agggcctgcg
    21541 cttgagccaa ggtgccgaac gtatagaatt tctccgggac cgccagatcc ggcttcatcg
    21601 tatcgaccag ctcgagaaac agcccactgt tcggtccacc cttgtggagt tgccccgtcg
    21661 aatgcagata gcggggcccg tatcctgtcg tcgtggccac gtgatatcgg accatgagag
    21721 ctttgcgtaa ggctcgcagt gccgcttcca cctgggtggt gggagtggtg taagccagaa
    21781 tggccaggta gcgattcgag cccatctgtt cgagcaattg agtcaacgct tgtttgggtg
    21841 gcagcacggc aatggagggc agtttcccct gagtttcgac ctctttcagc acgcgtcccg
    21901 tattgtcttt gctttcctgt acattgggct ggtcgaatgg atgaatccca agtgcatgtc
    21961 ccgcaatggc cgtcgcgaac tcccaccgga agaattcacc acccagatcg taacgatcct
    22021 tgagcggtat ggaatacacg ggctgtcccg ctcgctgcag ggcggaaacc gttctatcaa
    22081 gggaggcatt gcgatcgccc ctgaggcgaa gcgagataaa aattctatct gctccataag
    22141 cagcgggaga tgcgaatggc tctcccgcga cgggaatgag cccggtccct tctttcccgg
    22201 tgctttcggc gagcaactgt tctacccaga gtccaaaaga agttaatgcg ggcgaggtga
    22261 gaagggtgac cttgtcccgg ccgcttttgg ccatggccgc catcaggcct cccagggcgg
    22321 atcccggatt ttctttggcc accgtatgtc gacaggactc ccgcatggcg agagctcgag
    22381 tgaggagctt tcccacgtcc aacccaagca gggctgccgg caccaatcca aaatacgaga
    22441 gcactgagta gcgtccgcct atgtccggtt ggttagtgaa cgtccgccaa aatccacgct
    22501 cccgtgcgag ttcttcgagg ccggttgccg gatccgtaat ggcgacaaac tgggctccgc
    22561 cgcgatggcc ttttgtccgg tggacgagtt cccagaagtg agcgaagagg ctcatgactt
    22621 cgatcgttcc gccggatttg ctcgcgacaa ggaacaaggt tcgtgccggt tggatcgcat
    22681 cggtgacctg tcgcacccag ccgggaacgg tggaatccaa gacccagaga cgaggagcgc
    22741 ccttcgacga tccaaaggtg cagcgcagca cttccgggcc caaactgctg ccccccattc
    22801 caagtagcac cacgtctgtc gtgcgagcct ctcgagcggc ttgggcgaag cttcgcaagt
    22861 cggaaagtcc gtcttgcatg tggtcgagta cggtcagcca ccccagcctg ttatcgattt
    22921 ccgtcggatt cggcttccaa agggtatggt ctcgcatcaa aatccgttcg atcgcgcgac
    22981 tgcttgccaa ttcatcgaat gttgtcgcga gggtctgggt atccagaagg gaggtcactt
    23041 tggggcggat cggcataaca cgctctcttc cataggtgga ggaacgaaga tgctggtcgg
    23101 ctgagcccta tcttaggttc gaggatgtga aaaggcaaac caggaaggga aggcgatacg
    23161 cgatggcgga gtcagttgga gcgagagaga tgaatgatgg aggtggagaa gcgaccgaag
    23221 gctaatgcgt agcgggcccg acgctatgtt tcttcctcct gatcgcccaa gtcagccagg
    23281 actagcgaaa tggaattttg cgattccagt gcgccgacga tattgacggg tgtgcccacg
    23341 gcgacctggt caaacagttg ggcaatctgc gggttatcca gcacgatact gccgtacggt
    23401 tgatccgaaa ttccttgcgc gatgccatga agctgaatga ggcctggcat atgttggctt
    23461 ttggggatga gtcctgcttt ctgggcttcc tcgaatcggc gccggtcgtc tatattggga
    23521 tagtcgagaa tcagggcgcg gaaatagggc gtttgtccag gtccgcgttt acgttgaatg
    23581 cgatattcgc cttcgggagt agcgccatca ccgtagtgct gttttgcccg gattcctctt
    23641 gatcctaatc gaataggata ggtcagaact ttgcgaccgt tcttatatag ggtgagggag
    23701 cggtcggcct tgctgacgac aatggcctgg gcccgatgag ttcgagacca gtcaatggtg
    23761 cgttgtgccg attcccgcca cgccgcgatc agatcatcgt ccgcataccg gcctaactca
    23821 ttggtgagca gggcggtctg agtcaggagg attctaccgg ctcgttccga agcctggatc
    23881 gaatgttcaa attgtccctg ttcgaggaac aggcgagcct gtttgacgtg cacttcagtt
    23941 tcgaccggat gctcgcccag caccacgcgg ctgccgatgt ctcccactcg agagttcagg
    24001 aggcgaagcc gctgctcgat tttggcgagt ttcgtttcgg cagtcgtgcg ttgagcctga
    24061 atgcggctat tgacgtcgct caggagctgc gtgccctcga cttgaagggc ttccagatcg
    24121 gtttccagct cattgtcttc ccatggccat cgaacaatgt tctcctcgga tcggattcgg
    24181 ccttgtagcg caatccattg gcgggaaaac ttggaatagg ctgcggggtt cacgtcggag
    24241 gctcgcagtg ttgacaggtc acggtcgaga ctctcaatgg attccaccaa ctcctcagga
    24301 acggtttcga cgcacccgcc caagctcatc gataacagcg cgacgagggt aacgcgagtg
    24361 gcgatgcggc agatgtaacg tgagcggctc atcattgttg cattctagcg tgcggatggc
    24421 tgtcccgcga gttcttcccg ccttttgtcc ctaactggac ggtagtgtta ccgtggtgcc
    24481 ttctgatacg agggctccgg cctatccgcg gtccttcatg gattccgcgt gaaggtcgat
    24541 gattccatca ttccaacatc ctcctacgca atgtaagtgc cagaaagtgc attcgtgcga
    24601 agaccattga atgccttggg agaacaataa gttacggtgc agtattccgt tgtcttactg
    24661 gctggccgct gtggcatctt tgccgcaatc gggcaagagt gcctcaaaga agtggcgatt
    24721 gataaggcgt gtgcgcatcg gagaggccgc gcgcttcatg cagtctgcat ttcaggggac
    24781 gatttcatcg cggagaggcg atggtagatt gattgacacc ctcctgtgac gcccctataa
    24841 tgcggcctct tcattgaaaa atgatctctc gagaaggagt gcgcaatgtc gttcactttt
    24901 aaacagccct cgaatctcaa gcgcaagcgg gaacatggat ttcggaagcg catgagcaca
    24961 aagaacggcc gcaaggtcat cgctcgtcgc cgagccaagg gccgcgctcg cctcaccgtc
    25021 tgattctttc ctgccgctag gcaatctgca tgtaggcaaa catccaggtg gtgggtagtc
    25081 accgggggtg gatactgtcg tcttagcctg gtcgtggaag aacatgggcg atacagcggg
    25141 cgatatgcgg cttcaaacga ttgaagcccg gtcgaacctg gtaacccgca gacaggtcgg
    25201 ttatgacggc agggcaggcg tctgcgtcgt ttttcttgaa gcgtagcggg gatatccagc
    25261 tcatcaagaa gaatgggcgg cgtgtttcaa caggattgtt caatctccag atctgtccgg
    25321 gtacgatgcc ccaagacacc atgatgggga ttgtcatcgg acggcggttt gggacggcgg
    25381 tgcggcgaaa tagggcgaag cgcctattcc gcgaattggg ccgtgcagtg cggccgacgt
    25441 tgagtgcggg gcacgcattt cttgtcttcc cgaagcgaca atgcctgaat gtgccgtttg
    25501 tggagctgca aagcgtatgg cgcagcacat tgcaaagaca gcggttggtt cgtgttgggg
    25561 atccctcgtc gtgaggtact tctgcatcgg cctgctgtcg gtctaccggg cctgtgtgtc
    25621 gccgatgctt ggtcccgcat gccgcttcca tccgacctgt tccgtctatg cgcaagacgc
    25681 cattgaacga tatggattgc tcaagggggt agcgatggct ggttggcgcc tgctgaaatg
    25741 tcacccgttc catcctggcg gcgtagaccc ggtacggtga tctttgtgac gacaacccct
    25801 caggtgttat tcggtacata aattcatgga aaagcgcgtc attgttttcc tcatcgtctc
    25861 tctggccgtc attatcggct atgactacct cctgaagggc atggggttgt tgcctccgtc
    25921 tgagcctgtg caggtcgcga cgaataccca ggtcgcggaa cgacagtctg cagggtccga
    25981 gaatgcctct gcccagtccg tgagtccggc atcttccact cagacaagtt ctccttccgc
    26041 acctggggca tcagacggtg gcacgggaac ggtcaaggaa gagcagacga tcgaggtgga
    26101 tacggacttg tttcgggcta agttctccaa ccgcggcgga gtgctcaagt cctgggagct
    26161 gaagcgatat accacgtcaa ccgatcaggg ccccgtgccg gtacagctcg tgtacgccgg
    26221 gggacgcttc aagggtcctc tgagtctggt cactaccgat caatctgtga cgtcagatct
    26281 cggatctgcc ctctaccagg tgacccaaga catttctcac ctcgatagcg cccatcccac
    26341 cggacacatc acattccgat atcacgacac gcagcggaat atggatgttg agaaggagtt
    26401 gacgttccat cacggatcgt acgtggtgga tatcgcggtt cggacgcaag gcctggccac
    26461 gcccgtggat gtcactctcg ggaccaattt tggcatcgtc gaatggggcg aaggatttat
    26521 tggcctgatg ggctcagcct ccctggttga tgacaaggtc ttgaaggaaa ccccggacgg
    26581 tgaagcggag cgtaagggag atgtgaagtg gtccgccatt caggacaagt atttcctgag
    26641 cgtcctgatg cccaagaagg cgatgtctgc gctggcgaag aaagagggcg ataagctggt
    26701 gtccgccgga gtcaggtttc ctgcgccgac tcccggatcg acaatgacga tgcagctgta
    26761 tgccggaccg aaagagtacg acaccctgaa gggcttaaat gtcggcttgg aagatacgat
    26821 cgactttggt tggtttgtct ttggcagttg ggcactggtg aaagcagtcg ccaagcccat
    26881 cttttatgtc ctgcgctttc tctacgagtt tacgcacaac tatggtgtca cgatcattct
    26941 cttgacgatg ctgatcaagc tgatgttcgt gccgctgcag tacaaaagct acaagtcgat
    27001 gaagcagatg caggtcattc agccgaaggt tctggccatt caaaataagt tcaaggatga
    27061 tcgcgagcgg ctgaacaagg agttgatcaa gctctacaag gatcatcggg tgaaccccgt
    27121 cgggggctgt ctgccgatgg tcctgcaaat gccggtcttc gtcgcgctgt ttaatattct
    27181 ctacatgacg atcgacctca ggcaggcgcc gttcatgctc tggatcaaag atctttccgt
    27241 gcaggatccc tattacgttt tgcccatcat catgggcgcc accatggtca ttcagcaaaa
    27301 gattacaccg accaccatgg acccgacgca agcaaagatc atgttgttcc tcccagtgtt
    27361 catgacgttc ctgttcgtca acttcccggc aggattggtg ctgtattggc tgacgaacaa
    27421 cacgctcacg attacgcagc aggtcgtgac ggagcggtta tttgggaaga agtggcaggt
    27481 ggtgcccgag ggcgctgacg ccgctgccat cgaagattcg aaagaacaga aaagtaacgg
    27541 taaacggcga aacgcctccg cgactgaata acaccacctc actgggtgcg tcatgcttgg
    27601 cgcgctagac gataccatct gtgcgattgc cacccctccc ggggagggcg gcatcggcat
    27661 actccgcatc agtggctttc acgcccttga cgtcgcgtct cacgttgtac gtctccggtc
    27721 gggtaacgcc ttgcaagatc ttcccacgca tcggctcgcc ctcgccgaca tctgcgctga
    27781 caatacggac gtacctgccg ctggtaccaa gcgcacgggg gcgttgcgtt ttattgatga
    27841 agcgctcgtc gtcgtgatga aagggcccca ttccttcacc ggggaagatg tagtcgaagt
    27901 gcaatgccac ggtggtcccg tcattctgga tcagctctgt cggggattaa tctcagcggg
    27961 agccaggctt gccgcaccgg gtgaatttac caagcgcgcg tttctcaatg ggcgtctcga
    28021 tctggcgcag gcagaggcgg tgctcgatac cattcgcgcg aagaccgcac gcagcctcac
    28081 cctcgcccag tcgcagcgcc ggggcgagtt atcccggcac gtggaggaga tccgatctga
    28141 attagttgtg gctctggcac acatcgaagc cgctttggat tttgccgagg aggatatttc
    28201 gtttgttcgt caggacgaat tgttgcgcct gcttgatgtg accctgcaga agctgcggcg
    28261 gttgcttcag tcgggacgtg atggtcgcat ttggagggaa ggcgcagtcg tggcgattct
    28321 tggacgtccc aatgtcggga agtccagttt gatgaacgcc ttattgcgaa gtgatcgcgc
    28381 catcgtcacg gctatccccg gtacgacccg agatctcttg gaggaatcgc tcagcattga
    28441 tggcattccc gtgcgattgt tggatacggc gggcatacgc gtgacggacg atcccgtaga
    28501 agtcgaaggg attcgccgga gcaggctggc ttggacagat gccgatctcg cacttatcct
    28561 cctggatggt tcccagtcct tgtccgagag cgatcgggcg ctcctttcag agcctgattc
    28621 gacgcgtgcg ttgctcatca tcaataaatg cgatctccct ggccaattgt cgagggagga
    28681 tcttgatcgc gcgtgtcctg gccatagcgg tattctcgaa atttccgcga cgttgtcgat
    28741 cgggctggac gaactgcgcg aaacgattcg tgggcggctg atgccgggga tgtgggagtc
    28801 gaatgaaggt gtgctggtga ccaatcttcg ccacactgtc gccattgagc gggctttgca
    28861 aggggttgat caggccaggc agtccgtcga ggctggccgt gcaggggaat tgatcgccat
    28921 ggatctccgc attgccgcag atgccctagg agaaattacg ggcgtcatta cgaccgacga
    28981 aatcctcgat cgcgtatttg cagagttttg tatcgggaaa tgaaagggtg tcgggcatga
    29041 gcgagcaatg tgacgtcatc gtggtaggag gaggacacgc cggctgtgag gcggccttgg
    29101 ctgcggctcg tatgggcgcg cgcactctgt tgctgaccat ggatcctgac cgtatcgccc
    29161 aaatgtcttg taatccggcg gtcggtggaa tcgccaaggg gcatctcgtc aaagagattg
    29221 atgccttggg cggcgaaatg ggccggaaca cggatcaggc aggcatccaa ttccgtatga
    29281 tcaacacgag taaggggccg gcggttcgcg cgttgcgcgc gcagtgcgat aaaaagattt
    29341 accgccaagt gatgcaggat acgcttcgcg cacagccgtg ccttcgcatc agaggcggaa
    29401 cggtcgatcg tatcctgaca catgccgggg ccgtggtcgg cattgtgacc gattcaggtc
    29461 agcacattga ggctcgggcg gtggtgctca cctcgggcac ctttttgaaa ggtctcattc
    29521 acatcggtct gagtcatttt ccggcgggac gagccggaga ggcttcagct gaacatcttt
    29581 cagattgtat gcgcgatttc gggtttgagg tgggccgtct gaaaacgggc actccgcctc
    29641 ggttggatcg cgataccatc gacttttcgg tcatggaact tcagcccggt gacgatccgc
    29701 cgccgccctt ttcttaccgc acgaatcgaa ttccccttcc gcaggtgccg tgtcatttga
    29761 cccatacgaa cacgcaaaca catgatatta ttgctaaaaa tatagacagg tcgccattgt
    29821 tcagcggcgt catcgatggt atcggtccac gctattgccc gtcgatcgag gataaggtcg
    29881 tgagatttgc cgacaaggac cggcaccaga ttttcattga gcccgagggg ctcgatacga
    29941 atgagttcta cccgaatggc atttcgacga gtttgccggt ggatgtgcaa gcagccatct
    30001 tacgaactat tcccggcttg aagcaggcca aaatcttaaa gcctgggtat gcggtggagt
    30061 atgattattt cccgccgcgg cagctccata attcattgga gacgaagctt gtgcaggggc
    30121 tttaccatgc cggtcagatc aatggcacct caggctacga agaggccggc gctcaaggca
    30181 tcatggctgg tattaatgcc gcgttgaagt tgagaggaga tgcgccactc gtactcgacc
    30241 gatctcaagc ctacatcggt gtattgattg atgatttgat cacgaaagac gcgagagaac
    30301 cctaccggat gttcacctca cgcgcggaat accgcttgct gctgcggcac gacaatgcgg
    30361 atcttcgcct gatggatctc gggcaccgga tcgggctcat cccagatagc gtccatcata
    30421 aatttcaaga gaagcgtttg gccatcgagc gcgaagtgca gcgactgaag gatacgaggc
    30481 cgaagttaac gccggacgtc aggaggctgc ttgaagaatt cgagattggg ccggtgacgc
    30541 cgacgcaatc gttggccgag attctcaggc ggcaggatct gacctatggg aagatttctt
    30601 cggtgttcgg cgggtgcgag attgaagatg ctgatattat ggatgcggtt cagctagagg
    30661 taaagtacga gggctatatc aagcgccaac agcagcaaat ccagcgatca caaaagttcg
    30721 agcacagggc gatcccagag agctttgact atgacgatgt gtctggattt tctgccgagg
    30781 taagggaaaa gttcaaacgt gtaaggcccg catctgttgg gcaggcatca cgaatttccg
    30841 gagtcacgcc tgccgcaatt tcgctgttat tggttgcgat cgagcgccac aagcgtcagc
    30901 ccctcactgc tgaatgattt cggtattccg ctctctttct ggccagcctt cctaatttgc
    30961 agaaacattc ttgacccttg gtattggttt cgcgtaattg ttccacgtgg aacacgacgc
    31021 cagccagttt gaagtatctt tccgaagatt cgccgcagag ctcggcatcc ctgtagatag
    31081 ttcttgtctg ccgttttttt ctacctacct ctcagagctc aaaaaatgga ataaggcgat
    31141 caatctgact gccatccatg atgatcagga agtcctggtg aagcatttca tcgattctgt
    31201 ggctggggta aaagtcattg aaaatatgga caaatgtgag cttctagata ttggggctgg
    31261 ggctggattt ccaggtctgc cgcttaaatt tgtgctgccc caaatagagt tagaactgct
    31321 tgagccgagc gagaagaagg gggcctttct gaggtatgtc gccgggtccc tcgggttgag
    31381 gcatgtcact gttgtcccat ccaagttgga gcagtacgtt cgccgaccga ctcggcgact
    31441 gttcgattac atcgtagtgc gagctttcaa ggtagacgag agtggccaga tggtggccga
    31501 tctcctcaaa ccagccggaa aagtgatttt atatcgagcc gagcgggttc cgagaggttt
    31561 tagactgggt ggccttgctt tggtgcagga agtcgagtat gagcttccat ccggatatgg
    31621 acatcgcgtt ctttccgtat tctcgaaata gaaggccagc tcggatgttc cacgtggaac
    31681 atcgcagtcg gttcagtgtg ctgctagaag attggggatg acatggcgag aattattgct
    31741 gtcgcaaatc agaagggtgg agtgggcaaa accaccacct cggtgaattt ggccgcggcc
    31801 ttggcaattg aggggggatc cgtgctcctc gtggatattg atcctcaggg taatgccacc
    31861 agtggcctcg gtgtcgacgc catgtcgctt ggaaagacca tctacaatgc cttgattgtg
    31921 aaagaaagta ttaccgatct ggcgatgccc actgcggtta acggcttgtc gttagtgcct
    31981 gcaaattcac atctagcggg cgctgaggtc gaactcgtga atatggaagg tagggagcag
    32041 cgcctcaagg aggccttagc cgaggtggct gatcgatacg acaccatttt gttggactgc
    32101 ccaccggccc ttggtcttct taccatcaat gccatggtcg ctgcgcactc cgttctgatt
    32161 ccggtgcaat gcgaatatta cgcgatggaa ggtcttgggc gactcatgga aagcattgag
    32221 cgcatgagac agtcattaaa tccgagctta gaaattgagg gcattgtgct gacgatgtat
    32281 gacgcacgta attctttggc gcgccaagtc gtcgagcaga ttcgcggcca ctttggcgaa
    32341 gcggtgtatc aaaccatgat tccgcgtaac gtcacgctgg cggaggcccc cagttacggt
    32401 cgtccggcgt tgctctacaa catggcatca tccggtgctc aagcctatct ttccctagcc
    32461 aaggagtttg tggtccatgg agaaaaaagc cctcggtaga ggtctcgacg ctctgttgcc
    32521 gactggccga aatacggccg atcccgagcg aggcgatgtt caggagttgc gcttggaagc
    32581 ggttgtcccg aatcgttttc agccaaggca gcagttctcc gaggtcgagc tggctgaact
    32641 gacagcgtcg ctcaagcaaa atggcctcct gcagcccatt ttagttcgcc gaaagggcga
    32701 cggcatctat gaattgatcg cgggagagcg gcggctgaga gctgctaagt tggcgggaat
    32761 gcagaagatt cctgctctta tccgaaatgt gtcggaccag gaatcgatgg tgctcgcgct
    32821 ggtggaaaac cttcagcgtg atgaccttaa tccgatggag accgcccggg cgtatcagcg
    32881 catgttgaat gaattcagcc tcacgcagga ggcgattgcg caaaaggtcg cctgcgatcg
    32941 gtcatcagtg gcgaacatgc tgcgcctgac ctcgttgccc gccgaagttc agcatatgat
    33001 cgaaacggat cagttgtcga caggccatgc gaaggtcatc ctcggtctga tgaccccggc
    33061 tgcgcaaatt agtttggcga cccagatcgt gagcgggcag ttgtcggtgc gagaagcgga
    33121 gcgattggtt caagggcacg cagaggctaa aaaagcaggg aagcgtccgg tgcgggcgcc
    33181 gttgcgatca gatctcgagg agcggttgca gaaacggctc ggcaccagag tcgatgttca
    33241 gaaggggcgc cgtggcggaa agatcatcat tcattatttt tctcctgaag aactggacgg
    33301 cgtggttgag cagttgctga acggatagcg gaggcatttt cagggcagat tttacggttt
    33361 gaccggagta caatgccgac ggaatttgtc tcgtcatcga agtggacggt gaagaattcc
    33421 cgctccgccg gtcagacgtg tggttatttg tttggaggta tgcactatgt ggattaagga
    33481 caaaccagaa ggaaagcgcc aggcaggaca aagcgaggga agtgaaatgg aaaatttgga
    33541 atcgacagca ccccgtgaag ggaatgaaga gatcaatgcc tttgtcggga agggtgtcag
    33601 ctttaagggc gtcatttcct acaacggcac cgtgcgtatc gatggcaatc tggatggcga
    33661 aattcacacc gaaggtgttc tgttggtcgg tgaggacgcg gtgctgaccg cgaagatcac
    33721 ggccggcacg gtggtgtgta agggaaagat caccggcgac atcagtgcgc gtgagaaagt
    33781 gaagttgcgc gcgcctgccg tggtgaacgc gggtgtaaag gctccgttgc tcgccatgga
    33841 agagggcgtg gtgttcaatg gtacgttgga gatgagccag acggggaccc gcgaaccgca
    33901 aggtacgtca cgcggacagg gcgtaaagct cgtcaacggt tagtccggct cgatcacgaa
    33961 tcataggaca gcgtgcgacc ggccttatgc cggatcggca tcaatagagt tccattcagt
    34021 ccgcgccacg ggcggtcacc gtcgtggggt gcgtgaagga ggacgctgat gtggggtgag
    34081 accaaaaagc agtccgtcgc ggaatccgac aagtttacct ttctcgggaa gggaacaagt
    34141 tttaagggca tcgtcacctt tgacggcacc gtccgtatcg atggccgcgt cgaaggcgaa
    34201 gtccacacag gtggtgcggt gatcgtcggg gaaagcgccg tgattaaggg cgtgattgcc
    34261 gccggatccg ttgaaatcag cgggcgtgtg aagggatcga ttactgcgca gcagaaggtg
    34321 gaaattcaga agcccgggat tctcatcggg gacatccaaa gccccgtcat tatgatcgaa
    34381 gaaggtgccc attttcacgg catgagcaac atgggcgctg agaagtggtc ggaggacgag
    34441 gcttccgaat tcggttctcc gcaggatccg ggcgtgcgtg accttgtggc ccatcgcggt
    34501 aaagtcaaaa cccaagagcc gtaagtgcgc ggctccttgt tcccccggta ttttttatga
    34561 cacgtcagac ggtgctgcta gggatgagcg gcggagtcga tagctccgtc gctgcatcgt
    34621 tgctcgtcca gcaagggtat gatgtccatg gcgtcacgct ccaggtctgg gagcacgaag
    34681 acgagcatgt cgcggtgtct aagcgatggg aggagcgggg ctgctgcaag atcgggattg
    34741 ccaagttcgt cgcgcaacgc ctacgtattc cttttgaggt cgtcgatcgc cgggaaattt
    34801 ttcagcaggg tgtcatcgat gactttgttg ccggatatgc cgcaggaacc acccccaatc
    34861 cctgcgtccg gtgcaatgag cgggtcaagc ttcgttctct ctatgccctg gcgcaggagc
    34921 ggggtatgga ctacgtggcg acggggcact acgcgcgtgt ccagcaggcg ggcggacaat
    34981 ggtcgttgca tcgggcactc gatgcgcgca aggaccagag ttattttttg tatcggatcc
    35041 aggccgactg gctgccgcgc ctcttgtttc ccgtcggaga gatgcaaaaa agcgacgtgt
    35101 ggaaagaggc ggaaaccctc ggattgcccg tcgaggagct gaaggagagc caggagattt
    35161 gttttgtgag ccaaggcgac tatcggacct tcctcgagca ggaacggccg gaactcaagc
    35221 agccgggagc ttttgtgggt gtcgatggtg aagtgttggg gcgacacgaa gggatcgctt
    35281 tttacacgcc gggccagcgg cgcggacttg gcattgcggt tgggcagaga ttgtacgtgc
    35341 agaaagtcgt ccccgagtca ggcgaagtag tgttgtgcgc ggaagaacag ctcgtgcagt
    35401 ccgattgtca ggtcggggat atcagtttgt tcgataggag catcggtcga caggccgtcg
    35461 atgtggatgt gaaaatccgg tatgccactc cgccggccgc cgcgacgctg ttgccatcaa
    35521 ccaacgggac cgtgcagatt cggtttcatc agccgcaacg agcgctgagc cccggacagt
    35581 ccgcggtctt ctaccaagga gatcgtgtgc ttggcggcgg catcattcag cgcacgtaag
    35641 gcgtggcctg ccgttgttcg cttttcagca cgtaaaaaca caatcaatat ttgcctggga
    35701 tgccttttgc ctcggtcttt tccgaaccgc tttgacggtc ctcctgcgac cattcctcct
    35761 gccagccatg ggatcctcct tgacagtacc cgcgtttgaa tgctaacttg gcgcgctttt
    35821 tgtcgtgccc accgcacgca agcttcccgt ttgatcaaaa cacggttaac cgtagcggat
    35881 agttattgtg attaagacag ccgtcgcacg tcgatacgca aaagctctgt tcgaacttct
    35941 tgatgcctcg agcattgagc cggcccgctt ggccctcaat ggactaggag aagcctttac
    36001 cgagtcatcc gcattgcggc atgccgtggc ttctccaatg ttccctgaag ccactaagtt
    36061 aagtgtgctg atcgaagtcg ccactaagct cggatgcccg ccggtcgtca agcggttcct
    36121 agatcaactc gtcaaaaaga atcgtgtcag tttcctgccc gacatcgccg atgcctttgc
    36181 caagctggtg gatgaatcca aaggcacgca gcaagtgctc gtgtcatccg cgaatgcgtt
    36241 gccggcgact gagcaggatc gcattaccac ccgattgcgc gatctgctga agcgcgatgt
    36301 cgatgtcact ttccatacag aacctgaaca tgtcgccggc ctccacatcc gcctgggcag
    36361 caccgtcgta gacagcacgg tccggggccg actgagcgcc atgcagcgag tgttgaccaa
    36421 ggagtagcca tgcagatcaa ggcagaagag atcagctcca ttattaaaga gaagatcaag
    36481 ggcttcgatc aacaggtcga tgtcagccag accgggtccg tgatccaggt cggcgacggt
    36541 atcgcgaaag tctacggtct cgatggtgcg atggccggag agatgctcga attcccgggc
    36601 ggcctctacg ggatcgcgtt gaaccttgaa gaagacaatg tcggcgccgt gttgatgggc
    36661 gatgacactg gtatcaaaga aggcgatccc gtcaagcgga ccggccgaat tgccgaaatc
    36721 ccggtgggcg aagccctagt cggacgcgtg gtcaatgcca tcggacagcc gatcgacggc
    36781 aaggggccca tcaattcgca acattcctcc cgtatcgaag tagtggcgcc tggcgtcaat
    36841 accagacagt ccgtgcgcga accattgcag acgggcatca aggccatcga tgccatgatc
    36901 cccatcggtc gtggtcagcg tgagttgatc atcggagacc gtcagaccgg aaaaactgcg
    36961 atcgccgtcg acaccatcat caatcagaag ggtctcggcg tgttctgcat ttatgtcgcc
    37021 gtcggacaga aacggtcgac cgtcgcgcga gtggtgaaga cgctcgaaga aaaccacgcc
    37081 atggagtaca gcatggtggt ggcggcaacg gccagcgacc cggcgccgat gcagttcctg
    37141 gccccgtttg cgggtgccgc aatcggtgag tatttccgtg acaacgggaa gcacgcgctg
    37201 atcgtctacg acgatttgtc caaacacgcc gtcgcctatc gacagctgtc gctcttgctc
    37261 cgtcgtcccc ccggtcgtga ggcctatccc ggcgatgtgt tctatctgca ctcccgtcta
    37321 ttggagcgcg ccgcgaaatt gagcgacaag ttgggtggcg gcagtttgac cgcgctcccg
    37381 atcatcgaga cgcaggcagg cgacgtgtcg gcctatattc cgacgaacgt catctcgatc
    37441 accgacggcc agatttacct tggtagcgac ctgttctatt ccggtattcg tccagccatt
    37501 aacgtcggtc tgtccgtgtc ccgcgtcggc ggttccgcgc agatcaagac catgaagcag
    37561 gtcgccggta cgctccgact cgatctcgcc cagtatcgcg aaatggcggc cttttcccag
    37621 ttcggcagtg aactcgataa agccacgcaa atgcagttgg cccgcggtgt ccgcatggtc
    37681 gaactgctca agcaagggca atacaagccg atgccggttg ccgaccaggt cttgtcgatt
    37741 tatgcgggtg tcaacggatt ccttgacgat gtgccggtgg acaaggtgct ccagtttgag
    37801 gccgatctgc tccactatgt cgcgcagaat caccaggata tgcgaaaaga cattgccact
    37861 gtcgggaaga tcgacgacaa ggtgggtggc aaactcaaag aaatcatcac gacctttaag
    37921 cagaaaatgg gctatggagg aaagtaagat atcgtgattg cgtgatcggg gagtgaccag
    37981 tcctctcttc actgactcgt ccaatcgcac ctgtctgctt ccgtaaaata tgccgagctt
    38041 acaatcactc aggcggaaaa ttgccgcctt caaaaatacg cagaagatca cgaaggccat
    38101 gaaaatggtc gcggcggcaa agctcaagcg ctcgcaggat cgtattctcg ctgcccggcc
    38161 gtacgcgcac aaaatgcgcg gcgtgctgag caatttgagc cagcgcgtca atcgtacctc
    38221 ccatcccttg cttcaaaagc gtgagggaaa gaaaatcgaa atcctcgtcg tcacgagcga
    38281 tcgcggcctc tgtggcggat tcaacggcaa catcgtccga aagagcgccg aattcgtgcg
    38341 gcagtgtgaa tctcaaggac tctcggtcaa tctcagcctg atcggacgca aggggcgaga
    38401 ttattttcgc cgtcgttcct tgccgattcg ccaagagtgg acgggcattt tcgacaagct
    38461 gagttttgag cacgccatcg atattggcgg cgatctcacc gaaaatttcg tcaaagggac
    38521 attcgacgaa ctctacgtgg tgtacaacga gttcaagtct gcgattcaac agcgcgtgat
    38581 tgtcgaaaaa ctgttcccta tcgacgccgc tacggagttc ggtgcgtcgc aagccaccga
    38641 gggttcgtcc cttggaggca gctatctgta cgagcctgat gagggcgagc ttctgagcgc
    38701 attggtgccc aagcactttc aaattcagac gtaccgcatt ttattggaat cggcggctgc
    38761 agaacatggc gcacgtatgg cggccatgga cggcgccacc cgtaatgccg gacaactgat
    38821 caagaaagtg accttgtatt acaacaagac tcgccaagcg gccatcacca aagaactcat
    38881 ggacatcgtg ggtggagcag aggccctgaa ataaatctgg actgaggaat gctgcacgac
    38941 ggtaagctgc cgggtgcatc agtgacgaca agaggagcgt tatgagcaca ggaaaagtca
    39001 ttcaagtcat cggccccgtg gtcgacgtgg agtttcctcc gggtcaactg ccaaatatct
    39061 acaacgcgct gaaggtgacg caagaagaga ataaggcggc cggaaagcct gccgtgcgga
    39121 tcactctgga agtcgcctct cacctcggag aaaatcgtgt gcgcggcatc gcgatgtcga
    39181 ccaccgatgg tctcacgcgc gggatggacg tgaccgacac cggtgccccg atcgcagttc
    39241 ccgtaggccg cgaaacgttg ggtcggctga tcaacgtgct cggcgaaccg gtagacgaaa
    39301 agggtccgat caagaccaac aagacgtacc cgatccatcg tcccgctccg agattggaag
    39361 atcaggacac gaaaaccgaa gtgctggaga ccggcatcaa ggtggtcgac ttgttagagc
    39421 cctacagcaa gggcggaaaa gtcggcctct tcggcggtgc cggagtcggc aagaccgtca
    39481 tcatcatgga gctcatcaac aacatcgcgc tccaccacgg tggattctcg gtgtttgccg
    39541 gcgtcggtga gcgtactcga gaaggcaacg acctttggca cgaaatgcaa gaatccaagg
    39601 tcatcgatcc cgatgaccac accaagtcga aagcggcctt ggtgtacgga cagatgaatg
    39661 aaccgccggg agcgcgtctc cgtgtggcgt tgaccggact ggccgtagcc gaatttttcc
    39721 gtgacgaaga aaatcaggac gtgttgttgt tcgtggataa catcttccgg tttacccaag
    39781 ccggttctga ggtgtccgcg ttgctgggtc gtatgccgtc agcggtgggt tatcaaccga
    39841 acctgtccac tgaaatgggc gcccttcaag agcgtattac ctcgacgaaa aaagggtcca
    39901 tcacgtcggt gcaagccatt tacgtcccgg ccgacgactt gaccgacccg gcgccagcca
    39961 cggccttcgc gcacttggac gccaccacgg tgttgtccag gcagctggcc gagttgggca
    40021 tttaccctgc ggtcgacccg ctcgattcga catcacgtat tttggatcca caagtgatcg
    40081 gcgaagagca ttacaaggtg gcgcgtggtg tgcagtccgt gttgcagcgg tataaggatt
    40141 tgcaggacat tattgccatt ctcggaatgg acgaactgtc cgaagacgac aagatggtcg
    40201 tggcgcgtgc gcgaaagatt cagcggttcc tgtcgcagcc gttccatgtg gccgaagcct
    40261 tcacgggcgc acccgggaaa tatgtgaagc tgaaggacac ggtccggagc ttcaaggaga
    40321 tcctggaagg gaaatacgat caccttcccg aacaggcctt ctacatggtc ggtccaattg
    40381 aagaagcggt cgcgaaggct gaaaagatgg gagtgaaagt ctagcagcca ggtgcccttg
    40441 ggcttgcagg ccgcgcacat tcagaaggtg cccggccgat gcgcggacga cagggcatcc
    40501 tggtgttagc cttttgcgag agagcaggaa aaggacaatg gctggaaaga ttctcctaga
    40561 agttgtgacg ccggaaaagt tgctcctgag ccaggaggtt gatgaggtca tcgccccagg
    40621 cagtgagggg gaatttggtg tcttgcctgg ccattgtcat ctgctgtcga gcctccgtat
    40681 tggagagctt cgatataaat ctcaagaggt gtggcactac atgtcgatcc tctgggggta
    40741 tgccgaagtg acgccggtga aggtcaccgt gatggcggaa attgccgaaa aggccgaaga
    40801 tatcgatgtc ggccgtgcgc aacaggctgt cgaaaaagcc gagcaacgct tacaggccgg
    40861 cggtctcccg tcagaagtga gggaagcgga aatcagcctc gaaaaagctc gccttcgcaa
    40921 aaagatcgcc gaccgtgcgc gaaaaacagg acacgcttaa gttctcgcct ggaacttctt
    40981 ctcgttccgt atctgccgtt cttccttatc gagctcgtct aggtcctcaa gtactgccta
    41041 gtagactctc tcgctatgcc tggttcttcc ttttcctatt gatatgctcc ggctgtgccg
    41101 gccctgtgct gctgagtcga gacgtgcaac agcggtccga ctggtttgtt cgcctcgatt
    41161 cggttcagtc cactgaacca tctccagtag tctatgatca tccattttca tggaaggccg
    41221 aagagctctc cgccatccta tccaggctct ttctcgaaga gcgtgtgggc ttaatggata
    41281 atgcaaaacc cgcaaagcca gtatttacgc cggtagaaat tgacctcgtc gagccggctg
    41341 ttcgagaagc ctttgggaag gcgcgcccag gggagtggat cgcattcctt ctcttgcagc
    41401 cgaacgccgg cgccaccgga gtgacatcag gcggtctctt tctcgaatct cataggttgc
    41461 atgtcgtggt ggccaaccac cgaacgatgg tcggaaacaa ttcggatgaa ttggtacgag
    41521 tgcgagctaa cccgttttac tccattcaag ggagcggcgg agtactcgca ttcgaaccgt
    41581 cgaggtttag actctccact aaagccaact ggtctggtgg tcaccgggct tcagccagcg
    41641 aacttgtcct cgaccattca gcattcctct cctatgtcac gcacgcggca ccacgcgaga
    41701 tgcccaccag cgctccgtca ccaatcagac agcagagctc agcggaggcg ctcacgtctc
    41761 ccgctgatgc atcagtccag ggcggccctc acgaccagcc catgctgcct cagattcagg
    41821 gagaaatcga acggctgaag caggaactgg cggagaaaga cctggaaatc gatcgtctca
    41881 aggcccaatt actcaagtcc ggagccagac cctgatcgag tttcgtgtcg atactactgc
    41941 cattcacgtt gtcaggctat tctctgtccg ctaagggttt ctcggcgaac ccccaaacct
    42001 tcagagcgat tactgctcgg tgttctggcc aattgcaatc gtccgccatg ctgccgtagc
    42061 ctagcgggcg atgcagacag aatttctcat ttcgccgggc gaacaaccga aacggctgga
    42121 tatctttctg gtcaaccgtg agccgaaact ctctcgtgcg gcggtgcagc ggatgatttc
    42181 agccggacgg gtgcgcgtga acaatcagac ggctaagccc agtcagaaaa taaagccggg
    42241 cgatgtggtc aacctcgaca aacccaagcc gaccccctta ctgatcaatg ggcagccgtc
    42301 agcactagag attctgttcg aagattccgc ctgcctcgtc ataaacaaac ccgcaggagt
    42361 cgcagcccat ccaggccccg gtcattggga tgacacccta ttgaatgcgc ttctcgatca
    42421 tgccttgcgg gcgggagtcg tcggtatgtc aggactcgtc catcggttag acagagacac
    42481 ctctggcgtc atggtcgtgg cgaaaacaaa gaccgcgcat ggacggcttg cggtgcagtt
    42541 tgaacagcac tcgatcacga gacaatacga agccctggtg caaggcgtcc ctcagcactc
    42601 ccatggccgc atcgagcttg cgctcggaac cgatcgtaac aacgtgaaac gaacctctcc
    42661 ccacacgctg catccaaagg cctcactcac tgagtattac gtcgaggagg tgttcggtgc
    42721 tgcggccgcg catatggtgc ttaccccgcg gaccggccgc acccaccaga tacgagccca
    42781 tcttcacgcg attggtcacc ctatcctggg agatccggcc tacggtgcag aacctgtccg
    42841 tgcgctggaa ggatatgggg tgccgcgtgt aatgctccac gcgtacaagc tgggtttcca
    42901 tcatcctctg acagggcgtt attgcgaatt tacggtgaag ccaccggccg attttcgcgg
    42961 actgagtgac gaactacgtt tcaagaccgt gagggaaggg cgaggacgaa cacagtgaat
    43021 agcctcatcc tccggcgtgt gtgatgagtg tgtgtgaatt cgaacactag atggagaggc
    43081 tggaaatatc gcgatgcaga tattgccgtt ggacgaggaa aaacggaaaa cgtccaaagg
    43141 ccaaatggca tcccaccaga taacatcctt gctgcgcgcc gagtggaagt gacgtcgtcc
    43201 atcggacctc gaacagactg gttcccggtt gtgagtgcaa cgtcggatta tgaaggctca
    43261 ggacctgccc ggtttgtggt tgttgatcga gcaacaaaag aacgccgccg gaactgacat
    43321 tcaatgagtg agccttgccc gaaatcacgg gagtttcacg ctgctgcttc ctgtccaatt
    43381 cgtaaaggca gggcgttctc accgcgaatc gagcatcgag ccgccgttcc cggaggacct
    43441 cagtcagtgg caggtctgtg atgttgtcca caaccagcgg ttcgactgac ggggtgggat
    43501 caaccgaact gcgtagcgat ctgaggattc tgtggataaa atttgaggac atcagatggt
    43561 ccttctcgct aaaaggtgcg acgcgaaatc cttcttataa gaaagcaatt caagtgcctc
    43621 aaaacgagaa aatattccgc acgccaaccg actgatttgt gtcggagtct gtttcgcgca
    43681 agcaccggga gtcataaatt acgcgtcatg gccccgtcac atttgccgtc cccctgacaa
    43741 agatgaacgg cctgacgata ggccgctgcc gggacgtgcc gaggatcgaa tagcacaaac
    43801 ttgtggatgg cttgtggatg gggtgtgtgc gacttgtgaa gcaaggtgga tatctcgaca
    43861 tcttgttttt agcccctccc tatggcagct tacccgcgct catgatgtcg gtcacagatt
    43921 aggcagttac cacgtgaata gagtcagcac gacgccggtt gagattgtcg tcaccggggg
    43981 agaatcgtca aaacggatcg acgtctttct tgccaatcgt gacccgacct tttcccgctc
    44041 ggccttgcag cggctgattg gggagggccg cattcaaatc aatggccaac cagtccggcc
    44101 gagtcaaaaa atccggccgg gtgatcgaat cagactggaa gtaccgcgcc cggagcctct
    44161 tgatctgaag cctgaggcga tccctctgga aattcttcac gaagactccg acctcttggt
    44221 cctgaataaa caggcgggcc ttgtcgtgca tccggcaccg gggaattggt cgggaacgat
    44281 ggtgaacgcc ttattgcatc actttgccac cgcgggcgtc actccctctc acatcggcgg
    44341 aaaagagcgg ccgggccttg ttcaccggct cgataaggaa acgtccgggg tcatggtcat
    44401 tgccaagacc gatcaagcgc accgtgcgct ggcggcgcaa ttcaaattgc acacgatcac
    44461 ccgcgtttat gaagcgttga tttggggagt gccgaagaaa gggcacggcc tcatcgagct
    44521 ggctatcggg cgcgatacga aagagcgaaa gaagttttcg gcccgcacca ctaaccccaa
    44581 agcctccgcc acggactacc aggtggaaga gcgatatgga aagattgccg cacaggtgcg
    44641 gctttccccc aggacagggc gcacccatca gctgcgggtc cacctcacct cgatcgatca
    44701 tcccattctg ggcgataaaa catatggggg agcaaaagtc atgacggtgg caggcatccc
    44761 gataccccgg gtcatgctgc atgcccggac gctgggattc acacatccca cgggcgggga
    44821 gtatcatgag ttcgatgtgc cgtttccccc ggatatggag caggtgcagc atctccttcg
    44881 cgacgccacg acacaggggg ggggctgccg gcagtgatgt gcagtcaaca aaacaggagg
    44941 ccgacctttc aagtcggcga gggtgaggag aagcgaacga tggaaaccag tgccgtgctc
    45001 gatcggatcg gagagctgat tgcgcagctc aagggatatg ccgccaagtt accgacggtc
    45061 gtgcatctcg catccgtgcc gaccggcgtg aaacccaaac cggaggccgt ggaagcattt
    45121 gagcaggcgc tcttgcgttt tcgagagcag agtggccgat cgccattcaa gggattgcag
    45181 gagtatctgc ttgaatcgtt ggaagcgttc gaagtcggca atgtgctggg ctcggtgcag
    45241 ccgctgatgg cggtgcttga tcaggtggag cggatgcaac gggataagga aattaaggtc
    45301 accccggcgg aggacaagcg gatgggcgag taccgtgcgg cgctgatcaa gattctgccg
    45361 ggcaatcagc cggagttgga gggcgccgga cggggcatgt agccctgcaa tcagcagggc
    45421 tcattgatga cctctgaaat gaatcgccaa tgtggaattc agcactcaga gagcaggact
    45481 ggccgagcgg ttagtgtccc attttcggtc ccgcagcgtt ggcgccggcg gtgaccaact
    45541 gaaagcggac ggtcgagtcg cagtgtggac atttcgtgcg gaccgtttcc tcgtcgatca
    45601 cgtcgtagtg gtcctgcttg agccacatca attgaaaaca cttcggacag cttcttactt
    45661 gcgttcccat aggcttcctc ttacactacg gttaaccgat tactcccgtt cggagggatt
    45721 aatttagtac aagatgcctc ctgctctcaa gaccggccaa cgacccatcg cattttccga
    45781 tcgcaacgat ttcgacgcga cccaactgat ccatttgtac cggcaggctc cctgggccaa
    45841 gcatcgcgcg ttagagcagg cgcaagctat gctggcgaag accgatctcg tcatttcagc
    45901 gtgggacggc ccacgtctgg taggattcgg tcgagtcctg acggactata tctttcgcgc
    45961 ttcaatctgg gatgtgattg tcgatcgcga ctatcagggg caacagatcg gcactgaaat
    46021 cgtacggcgc attctcgacc atcccacgct gcagcaggtt gaattgttct ggttgtgcac
    46081 gcgcatgccg ggattctacg aacggctcgg tttcagcgca aaggagcaga ccggcatggt
    46141 ctggtctcga aagcaaccat ccactcagtc ctaacagacc ccctgtcctt cgctcccgtc
    46201 ccttctactt attggcacac tggcacgacg taccagccaa cggtgctgtt cggcaacagt
    46261 ctggcgtttg tgtctattct gcttgaatcc gatgcagccg cttcggtgaa caacagacgg
    46321 caaggaagaa gaggcttgtg gccaataaca caatcgatgg tccggacggc aggtcgaatg
    46381 cgtaggagag caacacgccg gtgacggcac agaaaatgcc gatgagcatg gaatacagcg
    46441 tcatttgcgt gaggctgtgt gtgagctggt aggcggtgga ggccgggatg aggatcatcg
    46501 cgaagacgag aattgcgccg accgttttta aggcaatcac gacggtcaac gccaccagtg
    46561 tgaggaggag ataaaaaatc tgtctcgcgg gcacgcccga cgccgccgcc atttcctggt
    46621 cgaaggcgat gaagtagagt tcttttgaaa gcagcagaat cgtccccaac accgcgatgc
    46681 tcaatccgcc aatggtcagc aactcctctg tggtgacgga cagcacactg ccgaagaggt
    46741 acccgtagac ttcggggtta tacgtcttca tgaggcccag aaacaaaata gccaaggcca
    46801 tggtcgcggt gtagaggatg ccgatggaca catccaactt catgcgcccc ttttcctcca
    46861 cccagccggt gatccacacc gtggcgagac cgaacacaat ggcgaggctg agaggcggca
    46921 ggccgagcag ataagccagc gtgacgccgg caaatgcggc atgggccgtc cctgcaccgg
    46981 cgaaagccag gccccgcaat actacaaaga cgccgatcac tgaacagacc gagcccacca
    47041 gggcggcggc cagcagtgag cgttgcataa aatcgtaggc gagtagctct aacattgtct
    47101 cactaggtca gtgaatcatt ggggaacgac ataacgaaca cgttgtatcg tgaatcaggc
    47161 tccatcatga gatcggaatg gtcagtggtg atgatggtag tcttccacga tgatgaagtc
    47221 tttatccgta atgaccaact ctttgccata gacctggctg agaatctccg gcttcagcac
    47281 gtcctgcggc ggaccggccg cgaataattt cgttttgagc aggaccaatc gatccacccg
    47341 cgagcgaatc atattgatgt cgtgggtaat catgagaatc gtcagtttca gttcctgatg
    47401 cagctgttgg atcagttcca ccacactgtg ctgggccgtg aggtccagcc cggtcgttgg
    47461 ctcatccagg agcagaatgc gcggctgttg tgccaaggcc cgtgcgatga acacacgttg
    47521 ttgctggccg ccggacaaag cacccaacgc ggaatcccgg tgatgatcca tgcctacctg
    47581 cgtgagcgcc tggcgggcaa tgtcacgatc tttcccggac ggccggcgaa acagacccag
    47641 cgcgccatat cgccccatca tcaccgcttc cagtacggtg acgggaaagt tgcgatccag
    47701 catacccttt tgcggaagat acccgatgag ggcgcgatgg tggcagcgca gttcctcgca
    47761 ggcacagtcg aggacgcgga gcgtgccgcg aatcggcgcc atcaatccga gcacggcgcg
    47821 gcacagagtg gtttttcctg acccgttcgg gccgatgacg ccgacgaatt ccgactcggg
    47881 aatggacaga gaaatgtctt ccagggccac ggtgccggga aaaccgaaag tcgcatggtc
    47941 aaaacggata atcggatggg tcatgcgtgg cgcgttcgat gaagatgctg gttgtcgtcg
    48001 atgtccggtt ggaggcggcg gttatgtctg ttcaagcgcg tgggccaatt ggagcacatt
    48061 atagcggagc atgtcgaggt aggtctcggt ccccgggaga ccgcccggca aggtggtcag
    48121 gacggcgatg tgcgccccgg tttcccgggc cagcagttgt ggaactttct gattcagttg
    48181 aacctcggaa ataatgaccc ggatatgccc gcgtttgatg gcgtcgatca gcgtatgcag
    48241 atgccgtgcg gagggttctc ctccaggctg agtgagaatc gttccagcaa tctgtagatc
    48301 gtaacgccgg gcaaaatagg gccaagccgg atgatgcacg atgaccgcac ggctcggcac
    48361 ctttttgagc cgctccagcg attctgcctg aacacgcagc agggcttgga gatagtcagc
    48421 cgtgttgttt ctgtattcgg ctgcgtgggc cgggtcggct tccaccatgg ccttggaaat
    48481 atgctgcacc atcgtggcgg ccgcggacgg atccagccac acgtgagggt taccgcccgc
    48541 atggtggtgc gtgtgggcat cggcagtcga ttcgggatga tccgggatca attcaatgtc
    48601 tttagaggtc gtgaccacct gcaaggaggc gtttcccgca ttcttgacca gtgaggatac
    48661 ccacacttcg agtcccgctc cgacctcaaa caagagggca gctttgcgga ccgcaaccag
    48721 atcgctgggc ttgggcgagt aggtatgctc gctttcatat ccggtcatga gcgagacgac
    48781 atggacatga gacccgccga tttgttgcac ccaatctttc aagactggga gcgtcaccac
    48841 gacgttgagc ggttcagctg ccgatgccgt gggtacagct gcggaaaggt ttcccagcat
    48901 ggtcaggaaa atgaaaagag tgagcaaaat cgtgcgaaac atccggcgga ccgtaacacc
    48961 cgatgtaccc aaagtcaacg cgatgacgca tgcaaaccga gtgctctgca gcagcgtacc
    49021 gcttgacccg tctcgtggtt tcgattagcg tagcgcccga atgccaggtc gatctcaggc
    49081 ggttgtgttg aatggggggc gggatgaagg ttgtcggact catgtccggg acatcggcag
    49141 atggaataga cgcggcgctg gtgaatatcg ttcggcgcgg aggccgaccg gtcatcacgc
    49201 ccatcgcctt ctccaccgtg ccctatcctc gcgccttgca gcagcgagtg gtggatctgt
    49261 cgcttcatgg ccaagtggcg gagatctgcc atatgaatgc gtacctcggc gaattgtttg
    49321 ctgaggccgc gctcaaggtg attcgggcgg cgaaacatca gccagccgat atccatgcca
    49381 ttggatcaca tggccagacg atccaccact tgccaaaagg acggcgtgaa tccggcgtcg
    49441 gcctgatccg atcgacatta caaatcggtg agccggcagt gattgccgag cgcacgggta
    49501 tcacgaccgt agccaatttc cggccgcgag atatggcggc tgggggtgaa ggcgctccac
    49561 tggttcccta tgcccatgcc gttgccttca gccatgcacg ccggacgcga ttggtggtca
    49621 atatcggcgg gattagcaat gtaacctatc ttcctgcagg cggcgtgatg gctgatgtgc
    49681 gcgcgttcga caccgggcct ggcaacatgg tgctcgatgc cattatgcag gaaacgacaa
    49741 agggcaaccg tacgtacgat gccggggggc gattggcgcg aaagggcacg atacaccaac
    49801 cgctgctcac cgaactgatg gcccatccgt tcctgacgcg gcgtccgccg aagtccacag
    49861 gtcgtgaaga gtttggtgcg cccctggttc gtcgcttgct cgcgaagcag aagaaagcgc
    49921 ggctgtcgat tgaggacctg ttggcgacct gtgcggcctg gacggcggaa gccatcggtt
    49981 catcgcggcg ctgggtcccc ggcaagatcg atgacgtcat cgtcggggga ggcggggtct
    50041 ccaatcgagc tatcatggcg tcgctccgga cggtgtttgc ccccgcaccg gtgctgacat
    50101 ttgatgagtg cggttggagt agtaaggcgt ttgaggcgac cgcgtttgcg ttgctcgcac
    50161 atgacgtatt gcacggccac tgcacgaatg ttccgcaggt gacaggggct cgtcatcccg
    50221 tcttacttgg atccgtggtt cccggcaaag cggacatacg gaggaggaac cggtatctgc
    50281 cgggttaagg gactcgcatg aacatggacg tactctatcc tctcctcgcg atcggcgcgc
    50341 tggtcctttt gggaagtctc gcgtgggaaa cattcgggcg gcgacgtgcg ccggctgcgt
    50401 catccggcag cctgccgtcc ggactgacct ttgcccccaa gcctctgctg acagaccagg
    50461 aagttcagct ctacaacctg ttacgccttg cggtcgaaga tcgatatctc ttatttactc
    50521 aaattcctct ctggagcgta ttggatttgc gcgttccctc cgggatgcct ccagccgaaa
    50581 cgctgcggga gctggcgctc acacgcgcca cgtttgtgct ggtgcatccc ggcagtcggt
    50641 tggtggagaa agtggtgcag gtcgagccct cggcctcgca ggcagaaggg caggggacgg
    50701 caaactctct attcgatggt gcgctgcgcg ccgccggcgt gcaactcgtc cgactcagtc
    50761 cattccatac gtatacggtt cccggcctcg tgacggtgtt ggatctcgcc gatccggatt
    50821 gaatccggcc gagtcagtga ggaagatcgg ttggtcgacc cgtagggacc gaatgcggca
    50881 agccggtcaa ttcggtccag ctgcgattgg atctcccttc gcagcagcgg cttctatggc
    50941 cttacgcgcg acttctgcct ccgggctatc aggatattcg ccggtgactc gctcgtacat
    51001 catttgagcc ttttcaaggt caccctgttt cgtgtagatc tgtccgattt taagggtcga
    51061 ggcggcgagt ttctggctca tgggatagtc tgaaatcagt ttgaagaatg atcccagtgc
    51121 ctcgtcgtgc tttcccatac ggtactggca ttcccccatc caatattgcg cattggccga
    51181 caatgatgag tgcccgtgga gctcaaggaa gaagcgaaac ccggcgagcg ctgcagggta
    51241 gtctttgtgg cggaactcgt ccatcacgcg atcgtagagg cggcgtgagg catccggttg
    51301 ctggggtgac gcggcgaaca gatcggatgc gctcgcgagg atgctgaaca acgccaatgc
    51361 tgcagcgata cctcgcacct tttcacggct gagttgaacc atggtcatag aagcccctct
    51421 ttcttctgtg gtcggcgcgt ctggaaccca tccattattg tggccgtgga tatgaaagaa
    51481 ccgcaccagc cggaggcaat cgacgaactt gttgtgaaat tgtatggttg gttaatccga
    51541 gcggctcggg gatcagaaag ctctgtgcgg aaagcacaca ggattgtacg gaaaagagcc
    51601 gaagggtatg ttgagggcag gcagttgaca gaaaacacgt tgtcgtttgt tggtggttag
    51661 cgctccattg tggagtgaat gacttggtcg agttcctaga ggattgaaac ggtcgtatgg
    51721 atacatctca cgaggctggt tcatcgggcg caggcaagga ccctctcatc gaaccgccac
    51781 ccaatcccaa aggcgggaag acattgattg tcgatgcccg cgattccgat gcctaccttt
    51841 gtcccagcgc ggcgctcaaa gacgcgggcg aggatgacca ggtatttgtc cgccccggtg
    51901 tgtatgaaga taaggtgttt gttgcggata agccggtgct tctcgtcggg gctggtcgcg
    51961 accaggtgca gatatacagt cgtcgcggag ggccactgta tttgcaacga gtgcccaacg
    52021 gcatgatttc cgggattacc ttccggtatg tgggcagcga tcaacattcc gccatgaacg
    52081 ttttagactc ggtctgcacc attacgcggt gccgggccat ggaaggcatc ttatcggggg
    52141 tggtgatcta cggtcccgag gcgaggccga cgtttatcga caatgaggtc tgttccaatc
    52201 gggaatccgg catctttgtc tttgcggggg cgcagcctcg ggtggcgcag aatctgtgtt
    52261 atggcaatca ccattttggt ttggcggtgc gagatccagg cagtcatccg gagtgtgttc
    52321 ggaatgtgtg tcatagcaac atgctgagcg ggatgctttt gttccatcat gcggaggcgc
    52381 tgctcttgga taatatctgc cgcgacaatc agcattgggg cttggtgatg acgccggact
    52441 cgcatccgac tccatcgcag gaggaattgg tggcggggaa cgtgttcctg cccaatcccc
    52501 ggggctcggt ggtggtgacg gagcagcctc tcgccgatat cgggcggtag cggtgagcga
    52561 tgaagagtag gtggattgaa ctgacctagc gcgcggcggc acgcgggccc tttacgggag
    52621 gggagcgggg acatcgggag aatggccggc cggtgagtga aaatgatagc ggcctccccc
    52681 ctgctgtttg gctgcgtaca tcgccttgtc cgcgtgcctc aggagctcgt cgagatcctc
    52741 gtcgtccgaa ggatagagcg tgatgccgat actcacgccg atcgagatat tatgcggccc
    52801 gatctgaaat ggcgagccaa tcgagtctac gattcgcttc gcgacaacca gcacatcgtc
    52861 ttcgcctgag atcccttcga gaatggcagt gaattcatcg ccgcccagtc gggcgaccgt
    52921 atcgacctct cgcacacatt ccatcaagcg tgtggccact tctttcagta gctgatcgcc
    52981 gatatcgtgt ccgagcgtgt cgttgacggc tttgaagcga tccagatcca gcagcatgac
    53041 cgccagcggc tgatcttttc gcttgctgcg tgccatggcg tgaatcaacc ggtcgcgaaa
    53101 cagggcgcga ttgatcaacc ccgtaagcgg atcatgctgg gcaaagtagg tgagccgctc
    53161 ctgcgccttc ttgcgttcaa tggcatagag aattgaccgt gccaaaaact cagttccacc
    53221 ttcccccttg accagataat cctgcgcgcc atgttggagc gcctgcagcg cgagcggatg
    53281 gtcatcatgc ccgctcaaga cgacgaccgg cacatcgggg ctggtcgcca agacctgtgt
    53341 gacggtcgcc aggccatggg tgtctggaag cgagagatcg aggaggacaa catcgaaccg
    53401 ctctcggctg agtcgggtga gtccatccgc caggacggag gcatgggtaa tctcgaaacg
    53461 gtccagtgac cattcggcta ggaggtcttg cgtcagttgc gcatcgacgg aattgtcttc
    53521 cacgagaagg agtttgatca tgaggccgcg ttcacactcc gatcacagtt gtaccggcag
    53581 gttgtgcaca tcatgatgaa tcgcaaggtt gggcgaggat tcagtgcggg gtctctcccg
    53641 gcccacggtt tcctggagat aacggaccgg cttgatcggg attctagcat gtcgcatcga
    53701 accgtcaaac aacagcctgc ttccccgcgg catgtgttgt cgagaccatg gtctgcgccg
    53761 tagcgatgtg cctggttagt ccggcgtgct cgcaacgttg tagattgcct gcaacggaca
    53821 ccaacgagct gattgccaaa ttacggccgc gggatggccc caaattggct catctcaaca
    53881 tcctgccctg cgcgatcgca atcactgttc agaacccgtt ctctaacctc ctgatgttat
    53941 aggccgtgac cagttttgta cgggccggcg aagtggcatg aattctgcgc aagaagatct
    54001 gggtcgcaac gcaatgccct gcacacacag tgcagaggaa ggggagggcc ctatggcgca
    54061 ggaacagtct gctcgagacc aggagagttt ttcccgcaca tcttctgttg atgactgcct
    54121 ggatggaaag acgcgcacat cagtctcctg ggcgcttcca ttggcgattg ccggggtgtt
    54181 ggttgtgggg gattccgcct ctgcggaaaa cgacatctac ggctatgtgg gagcaaacgg
    54241 cgtgttcgag atgacgaatg ttccgacgga tggacgcttt cgtccggctg agttgcagcg
    54301 tagccgattg tcacatcgag catcagtcga agaagtcgag gatgccgttg aacgctatgc
    54361 ctggcagttc cacctgcacc cagccctgct gctggctgta atcaaggcag agtcggattt
    54421 taatcccacc gtcatttcac gagctggagc ggtgggactc atgcagttga ttcccgaaac
    54481 cgccattcgt cacggtgtgc acaacttata tgatacgggc gataacattc gcggcggcgc
    54541 gcggcatttg cggtatttac tggatcgatt taacggcaac atccggctgg ctgtggcggc
    54601 gtacaatgcc ggcgaacggc gagtagaacg atatcacgcc ataccgccct atcccgaaac
    54661 tcgcgactac gtccggaaag tgatgatcta ctacaagagc ttccgtggag attatcaagc
    54721 cactccggga aaagcggtgc tcctggccat gcatcataaa cgattgcagc tggagcccct
    54781 gtcggtcccc gtggattttc gccatggtcg ccctgcagcg aaaaagtaac gagcaataaa
    54841 aggctgcagg cttccagggg cctacctgcg acaagactat cggaggcgaa agggcggagc
    54901 cccgaggtcg gctgttcgcg ggtctgtgat gaacctgcac tgggaggcta gcgccgacca
    54961 acgcgcagat tgctaaaaac tgctgagcat ccctctcgaa ataaaagcat ttaaaggaac
    55021 atcccgtact cgttcatcga ttggcgtgat cgtagtttca catctgaact tatatggctt
    55081 ccacaagagg gtgaagccgt atatactcat ttccactgtc agaattcagg aagatatctc
    55141 tgtcaatatt ggggcatgcc cgggtttcgc ctaggtaaaa caacaccgtt cctagggcac
    55201 tatgggcaag cgggcggccg ttgacaatag cgcgtcagcg cccggctgct aagctgatgc
    55261 gcaattcccg cacacattct ggggacgcga aaccctatcg ccatcgtaca gcttctacgc
    55321 cttgccggat tgtgtcgcaa aattagtggt cgcagtcgag aacagggccc agatgtaatc
    55381 ctgtaaatgg gatgcatcag ctagcccgtt tttgaggagc catatcttgc ctgacacccc
    55441 tttctccgaa tcacgcagcg gaccgcccag ccatggacat ttgtcgcttc gaagtctgtt
    55501 gatctctgca ctggcatggc catttaaaac attcatcagg cggctcatcg gagaagtgtt
    55561 ctatcagcag gcccgagtca cgttgcttca cgaacacggt ggtgtagggc ttgactggag
    55621 gtggggaaat tctctccggc gcgtccatcc tattcggcgt gatttcggct ggcagtcagg
    55681 acgccccatc gatcggtact ataccgaaga ccattttctc cctgcttacc aggcggatat
    55741 tcgcggccgg gtgctggaaa tcggcgatga tcgctacacc cgacaattcg ggggcgagag
    55801 agtgaccctc tcggagatcc tcaacgtgaa tccgggtatg gggggcacga ccatccaggc
    55861 tgacctcact gatgccaaac agattccctc aaacacttac gattgcgcca ttctgacttt
    55921 cacgttgcag tttatttttg atgttccggc ggcgctcaga acactcggcc gtattttgcg
    55981 ccccggagga gtgttattgg tgatcgttcc tgggatcagt cagatcgcgc gttatgacat
    56041 ggacaattgg ggcgagtttt ggcggtttac gtccctgtcg gcccgtcgac tctttgaaca
    56101 agtgtttcgt ccggaagaca tcacgatcac gacctacggc aacgtgctat cctcccttgc
    56161 gagtttgcat ggactccact ccacagagct caccaccgcc gagttagacc attgggaccg
    56221 cgactatgaa ctcgtcattg gcatacgtgc ggtaaagcgt ccagctgcca acacgagcct
    56281 ctagagctgc ctcgatctca agaaatgtct caatgcttac agggagggag gctgatggct
    56341 ccagggttcg ctgacgcctt cttcatcaag cgaatcacat gcctcctaga agaaactcga
    56401 atcagatcag tatgtggtgt ctttagtgga gataaccggg agggagggca ttcaaaatta
    56461 acgcatccgg caccatgcac agcgctcgga tggtcttatc aagggcaggg tcatggtatg
    56521 ttgcggccgt gaacgattca cgatctacgt aatgaggatt gagccgagag agtgtctcat
    56581 tgatgtagag tgacggatga gccaataggc cttgccgtaa tacttcattc gggagcgtat
    56641 ccgcatgata ttggcagata ccggagaggg ccggatgtga ttgaagaaat gtttccagcc
    56701 gccattcata atccagaagc tttgaaaaat ctctttctgg accaaactcc aaggtcatgt
    56761 cacccgtaca ccagaaaccg gcatgcccgt cctgcaaggc ttggttcaat ccttcctcaa
    56821 gcattccgag cattcgatca atactgaaat gcccatcggt gagatgggtc gggtttgaag
    56881 ataagacgag acggcctctt tcaacttctt gtggaatatc gattcccgca gccagcaaat
    56941 aggggcgcat ggcagcgatc atggccgagg tattgaaata caggcagcga taattctcgg
    57001 taagtttttg ccgaatgaca gccgagagcc cagaaaaatg cgtcgctggt gagcccttat
    57061 aaatcaggca ttgatggcga ggagtcgatg ccgtgatctc ggtgggtgtg gtcaacgcgc
    57121 tatgcatacc ctagatacta tcgcataagg gatatttgat cagctgaaat tgaataatca
    57181 gcggcggccg ctttcagtgt ccgccccctc cacggcgcgc tcccatcaat aatgtgtttt
    57241 gaatgtaggc tagttccggt ggctgagtct tgacgatcaa tcatgctact tggccgctaa
    57301 ctgagcggcg accttacagt tcctgctgga tgctcagctg gattttgcca caccgttagc
    57361 acaatcggct ggggtgaacc cctggggtga accccttcac tgagacagtg cctgaggaac
    57421 agcgacaccc cagctcgtgt ggcagggtga gggacgatct ttcgacaagt ggtgagattt
    57481 gagtccggtt tgagtagaat ggaagcagct agccgactca ttcagatggg cgagtgcgga
    57541 ggagggttcg acgatgcgac aaacaatcaa agcccgctac catgacggag tgctacagcc
    57601 gttggaacct ctttccctgg ccgatgacgc ggaagtgcag gtcacagtcg ataccgacct
    57661 tgcgctcggt acggacgaaa ttctgcgtcg tgcagcgcag gtctatcaag ggctgagtgc
    57721 cgacgagatc acgcaggtcg agtccatcgc attggatcgg cagcacttct tccgtgagcc
    57781 ggctgcctaa tgccgcaacc ggctgtcctc ctcgatactg atattctctc cgaactgctc
    57841 aagcaacatc ccctcgtcgt gcagcgtgtg cggagctatc tgacggagca ccagcggctg
    57901 gccttctcaa tcattacgcg ctatgagctc ctgcggggac tgaaggcgaa acaggcacgg
    57961 actcaagagg cagccttcac gttgctctgc caggccagtc tcattctccc gatcaccgac
    58021 caagtcgtcg agcgtgcggc gacgttatac ggcgatctcc atcgacaggg ggctttgctg
    58081 cccgatgcag acctgctcat tgccgctact gccttggacg cgcaacgtcc gttgattacg
    58141 aacaacctcg cgcacttcca gcgcattcct ggcctcatcg tcgaaagctg gaaacggtca
    58201 gtctaacgaa gcgatcctgc ttccttacga tctgcccatc gatcaaatcc gtacccttga
    58261 agtcggcaaa gctgtgtaac cggccccatg atgggattta ctatatagat tccaattccg
    58321 ctgcctggag gggaaaaccg gtcgatcgag aggagattgc ccaccggatc ataagtgtag
    58381 atggcggttg cgccggcact gtctgccaca ccgattaggc gctcttgatc atcgtaaagg
    58441 tatagggatt ggtccgccag gactagatat cccttgcaaa acgaaagaca cagaaaagcg
    58501 aagaagatcc tggttccgag aaaaaccagc aatcgtatct gcacgacacc tccagcttca
    58561 caaagtaaat aactaccagg cattggaccg gaatttatcg agcacgctct acaatcgccc
    58621 tgatctttcg gataaacgca tcaagcggtt gaggattctc ttcattgcga attgctaccg
    58681 gcatggcaac ctgctgcgtg atatcgactt tccctgcata accccgcacg gtggcgagac
    58741 ggccttgatc atcctcatcg acgaaaaata tgctggtatc gttcacatta aatcttgctt
    58801 cagtactcat tccaccaatg taatagaggt cgagctcatc ttgttgaagg cgtcccttta
    58861 tgacaaggtc aggtttaatc acagccttga acaaactcca tttgccgatc ttgaattcat
    58921 cagtaagtat ctgaccagtg aattccgcgc tagcgacctt gccgagcaca ataagtttag
    58981 actcgtgaac catagcttcc aaatcttctt cgacaatttt tccatcggag ttgtcgacat
    59041 acgaaagggg caaaacgact agcagtaaaa aggctagatt tcggacagcc atcagcatcc
    59101 cagtcattga gctcctctta ctgtcctcac ttcattgtcg gtggtctggt gaaacaattg
    59161 gcttctaatt ctctgacaat ctgttcacct gcatattctg gccctagcat gctggtgagt
    59221 gtatggagag tctcgtgaaa gattgtgcca ggcacactcc aacatccttg cgcattcagt
    59281 ccttggggag tcagccatat cacatttgtc cctagtgaat cgttttgaaa cccacatgca
    59341 ggccttccgt tgggagtata attcgatggc cagcaactta ttacaagatc ctcccatctg
    59401 gccttgaacg cttgtcgctg tgagcagttc aagcaattcg gcttattcat tgcggtatcg
    59461 taagcaactg ctgccattga tcgaatgaaa ccacgggtgt ccttatttcg acatgtggca
    59521 gagtcaatca taaacaaccc caaaggatcg ctcgcctgag ttggactgtt atttgtgtag
    59581 acatagaagt tgttttctcc ccctcgaaat cgtaggggat cttcagtgac aaagcgctgc
    59641 atatttggat ggtaatacct tgcccgataa taaataagcc ctgtctcgtc attatcccgc
    59701 cccgtatact gaaaagcatt gctactaggt gtgtctagtt gcgtagatac gccatagggc
    59761 tggtaggtat aggtggtctg acttgccccg gcggcattgg taagtgcaat agaagttcca
    59821 agagcgtcag tttcgtagaa ctcatcattg gcgcctttgc ggatgtaggg ctcatcgatg
    59881 ctgagacctc ggatgtatgt ggccgagggt gtggccccca tgatttcggc atagacatcg
    59941 ttcccgtcgt accaaaaccc agtcgtcgcc ccgttgattg tcttcgattt tcttcgtccc
    60001 aatgcgtcat aggcaaaact tgccgtcaaa cctgtccgac tgattccgct cagtctgttt
    60061 ctagcgtccc atgtgtaggt cgtcgtcaac ccgccttgcg tctccgtcgc tagattgcca
    60121 ttgctgttgt acgtgagatt cgtagtcgcg ctattccaac gtgtgagttc attcgccgca
    60181 tcgtaggcaa tgttggcggc ggccacggcc gtgggaagat tggagccggc cgcgttctgt
    60241 cgcaattgcg tgatccggtt tccacccttg tcgtaggtat aggtcaacgc ttcgatcgtc
    60301 gtcggcgtca gggtgtcacg tcactctgat cctatcccag aacgccggga ttaccttcca
    60361 aacttttttt tcgcgcacaa tcaaaaaact ggtcgatttt tggttgctgc atgttccggc
    60421 tctgaacggt gctcacaatt gccgctctga gcgcacgaaa gcgcacatat gcctccttct
    60481 gcgtacttcc cttcttgcct cgtcctcccg ataatttgtc ctcgatttgt cccctcgtga
    60541 cccctcctgt cctctagagg gttctttact gcgtggaggc gtttgcataa ggtctacgca
    60601 tgcaccgaaa cgatcgcaag aatgcgacaa cactccgaat ggagccctgt tcagaacaac
    60661 accgtcgtcc gtcgccggga gacaacgccg gtccgcgccg catgccgaca ggctgcgggg
    60721 cggagcggaa tggcccccgt cttggtaaca cgggggcgct acccacagcg ctccgtacag
    60781 gagttcgata gattccatgg attctgggtt cacgctcacc atcgattggc tggcctttac
    60841 ggtcttagcc agtaaccccc aagagaccat gaaggtcctc ggtggtgatt ggagtaaggc
    60901 taaaggaggg ttccgagggt atcccctgtc ctggatgagg gcagacggcc tgcgcggggt
    60961 cggcaaactg ggcaccaatg cccctcgtcg cccgaatgaa atccatgtgg atctgtcggg
    61021 cggcctggcg tccgccctga cactggacca aatccgcacc ctgctgcagt gggtgcacaa
    61081 gcaacaaggg catgtgacgc ggattgactg cgccttggat gatcgagccg gaacggtgcc
    61141 ggtggcaaca atccgagaag ccgtctcggc aggccaatgt gtgactcgtg cggctcaggt
    61201 ccggcatatc gtctccaacc tcacgcacgg gaccggagcg acgacgggcg agacgatgta
    61261 cttcgggagc tcgcagagtc agacgttact ccgcatctat gacaagcggc tcgaactcca
    61321 gagcaagggc caggagaact accaggacta tggaactcgg tgggaattgg aactcaagaa
    61381 ggatcgcgcc gagcaatgtg caagagcgtt agcaacttta gacgaagccg attggaagga
    61441 gttggtgatt ggcttgcttc ggtcctatgt agacttccgg cagatcccga aggacgccga
    61501 ggatgaagaa cgctaccggg ctccagtgtt ggagtggtac gccctcctga cgg
//
LOCUS       DAOQDS010000016        59735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_154_length_59735_cov_22.291974, whole genome shotgun sequence.
ACCESSION   DAOQDS010000016 DAOQDS010000000
VERSION     DAOQDS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 59735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59735
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154_length_59735_cov_22.291974"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PK589_05580"
     CDS             <1..938
                     /locus_tag="PK589_05580"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19101.1"
                     /translation="HRQLQEVTDRFFPETEWTSIDEFYADTTRLQTRHPDPRALGDAL
                     KADILHTTGLTCTIALASGKTAAKIAADAHKPDGLAVIEPGAEAAFLAPLPIRSLPGM
                     GPKSTEAVERLGVRTIGDLLLPRFESALARLLGTRLASFQSLARGLDSDPVVADREAK
                     STSHETTFDEDTNDPAVLEPIIHGFLETLAHELRLEGLAAGSFTLKLKDSHFRITTRQ
                     RKFATPLNYDPDMWPEIRQALGDLLQPRLRYRLVGLGLSDLVPAPEPLFDRRQRDAVA
                     ALDRLIERHGTGVVRLGALPQSEAGTRKRRPRTPD"
     gene            1028..1510
                     /gene="rfaE2"
                     /locus_tag="PK589_05585"
     CDS             1028..1510
                     /gene="rfaE2"
                     /locus_tag="PK589_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNJ19102.1"
                     /translation="MPTKVTTRDELVSLLAERRQRQQRVVFTNGCFDLMHIGHVRYLQ
                     AARNLGDLLVVGVNSDGSVRALSKGPDRPIVPDVQRAEVLAALACVDYVVIFSEPDPG
                     ALIAALQPDILVKGGDWPLDRIIGRETVEARGGRVQTIPLVPGVSTTTLVQRIRSTTA
                     "
     gene            1518..4991
                     /gene="mfd"
                     /locus_tag="PK589_05590"
     CDS             1518..4991
                     /gene="mfd"
                     /locus_tag="PK589_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNJ19103.1"
                     /translation="MPPVVPSHLTNWFAPAREALRTGSGKPCLMGLHGSTAGFGLALL
                     TLSAPAQPLADRSWLVVAKTDDEAERLYRDTLFFRTLCGQSGDDLALFPKWETLPYES
                     TVPHIDLVSRRMQTLNRLCTTARTVLFTSVPALTQRVLPALVFTESILQFQPNSDLER
                     EVLVSSLLRLGYRKGSVVEIPGEFSIRGGIVDIYSTAYPDPLRVEFLGDTIESIRFFD
                     PATQKSTDKIKQAWVLPARELIRPDDSPDALAPLAADAEWHAPSVYGAMDSLLDYFPQ
                     PPVLVLDQPGALKAHTAECWQTIEEGYLRHEDRTDPNPYPTPDQLYFTWDQLVTATQG
                     YATLALEPVTAPDASWEPVLTCPAQTPASVGLGQRGTAFSQTLEVLDRLREGGPVVLV
                     ARSQGQVGRLLALFGEHDRPAMEWKPAALSAGGAQKAPFSVLNGEVSSGFLSPDLRLV
                     VLTEEELFAKGARHKPQHKSKAATFLSSLEDLNIGDFVVHMQYGIAKYQGLRRLSVQD
                     FDSDFLVLEFAGTDKLYVPLDRLSQVQRYAGADAHVPRLDRLGGTSWAKTTARVKKDI
                     EEMAHELVDLYANRELVHRTSYGKDSMLYHEFEAAFEYEETPDQRKAIEDIAKDLAST
                     KPMDRLVCGDVGYGKTEVAMRAAFKAVEENRQVAVLVPTTLLAHQHYDNFAERFAPFP
                     TRVALLSRFQSPKDTKAIIKDTAAGLVDVLIGTHRLLQKDVQFRNLGLVIIDEEQWFG
                     VKHKERLKQLRTQVDVLTLTATPIPRTLQMTMASVRDLSIIDTPPSGRLAIRTQVLRF
                     SEKAIREAILRELGRGGQTYFVHNRVETMERMGAWLQELVPEARIVMAHGQMDSKPLE
                     AVMLKFFHREADILIASAIIQSGIDVPTANTIIVNRADTFGLAQLYQLRGRVGRGGEQ
                     AYAYFLVPDEGNLSDDAQKRLTAIQQFTELGSGFRIAAADLEIRGAGNLLGKQQSGHI
                     AAVGLDLYLQMVEQAVQRLRGQVVEEEPDPTLRLSVSAYIPEDYVVDSHQRLSLYKRL
                     SSCGQLGDLALMHGEIEDRYGPPPDPVERLFELMQIRLLAKQLRLSSVVEQTHAVVIT
                     FDPKAKVSETAVQALMDRYKKRLRFLSPLSFELQMPHDDWSLVFPELNATLQTLHVCG
                     TNNQEPRTGST"
     gene            5023..5919
                     /locus_tag="PK589_05595"
     CDS             5023..5919
                     /locus_tag="PK589_05595"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ19104.1"
                     /translation="MTSPQTPDISPWRARLTGTALALCALGSLAGCTEPPQEEPVIAM
                     INGRSITQAEFDIRWEELSQATRARYEKEGGKRRFLDELIMRELLMQEARKQGLDQSD
                     DIREKTLRYREQLILDELLKDRIKTKVEVSKEELDAYLEKHANQLLANPKVQVSVMLL
                     PNVYAAKDLKRQVEGGGNFTRFALRYSIDERSRAKGGDLGPYRKGLLEPELDALIPSL
                     HPGVLSDPIKTDKGYYLMKVSPLEPEILQADQATRERLRQELLAEKRRKRLDDVFAEL
                     RTGATIRMADAARYVTDEPGRP"
     gene            6072..7031
                     /locus_tag="PK589_05600"
     CDS             6072..7031
                     /locus_tag="PK589_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNJ19105.1"
                     /translation="MRPTSLQSLSRPALAGLCLYVWVGLFPAGPVDAAKLEDRIVAVV
                     NTDLIMLSELKRDLLPDQERLRKLYSGEDLERRLKTAEAMAVTKMIERKLQLQAAKNK
                     GVEVSDQEVTQAVQEMKKQGEALNQADPNTTRSVREQLTLMRVVDREVRGLIMVADSE
                     MKRYYEEHQDRFAYPEEYQLSQILIKPRTPDGLSVAQGRAEALLATLKQGEPFEDLAL
                     RFSDGADASRGGRLGLVRQGELIPALEQALTSVPVGQITGIVETAEGLHIVRVDDKKP
                     RQFRPFEQVKAEIQSLVFQQKTEDQYQIWMADLKNKAYIEIKF"
     gene            complement(7190..7894)
                     /gene="yihA"
                     /locus_tag="PK589_05605"
     CDS             complement(7190..7894)
                     /gene="yihA"
                     /locus_tag="PK589_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNJ19106.1"
                     /translation="MAHLSSVDQVDAHPALLPDAIIMKLLSAEFIKSSVSPEQFPADH
                     LPEIAFVGRSNVGKSSLINSLLHRKKLAKVSKTPGKTRAVNFFTVRTSDPKLPLLSLV
                     DLPGYGYAKVSKTMRAQWGPMIEQYLEQRQSLGAVILLIESRVWMPQDVMTIQWVQSL
                     GCGLIIVLTKADKLRQSERKPTLTEVRVACGLPAEVPVLLYSAETHEGRDALWGEIRA
                     QFNGLQKHESGTRELR"
     gene            7924..8700
                     /gene="mtgA"
                     /locus_tag="PK589_05610"
     CDS             7924..8700
                     /gene="mtgA"
                     /locus_tag="PK589_05610"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNJ19107.1"
                     /translation="MSKSTGSLFGRILFWMTVLIGLPAAALALYWLATLPDVSRLAKH
                     HPTETAVMEARRAQAKEQGHPLHIKFAWVPLSRISPALQRAVVAAEDASFFAHEGFDW
                     EGIKDAALYNLEVGEFKRGGSTITQQLAKNLYLSSERSLLRKAREALITRSLEHHLTK
                     KQILELYLNVAEWGQGVFGAEAAARHHFGKSAEDLTIEEAALLAAILPSPRRYDPIRH
                     TAYLTRRQRHIVRWLERGNGRKAASINRLPPESLEVVPVE"
     gene            complement(8732..9160)
                     /locus_tag="PK589_05615"
     CDS             complement(8732..9160)
                     /locus_tag="PK589_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ19108.1"
                     /translation="MPALFHLAFPVHDLAAAKQFYVEGLGCVLGRESPTAVTFGLAGH
                     QLVAHLSEDELDRQKGIYPRHFGLVLTREEEWQALADRAEAQHLTFYQQPRRRFPGTR
                     IEHRTFFLEDPSGNLLEFKYYVHESAIFGERDYTSVGEST"
     gene            complement(9201..10139)
                     /locus_tag="PK589_05620"
     CDS             complement(9201..10139)
                     /locus_tag="PK589_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HNJ19109.1"
                     /translation="MKRLRPVLLVHGGAGRRAMTVAQAACVERTLAEGHRLLQAGVPA
                     LSVVEEAIRLLEDSGLFNAGRGSNRQLDGVRRMDASIMEGRDLRAGAVASIEGILHPV
                     TAARLVMEKTAHVLLVGKSASRFADYFGLERAPRSRRSLTAQPKQLRPAAGVSRTLQL
                     HQAIVKTGRLRARSLGKETVGAVALDVKGTVAAGASTGGVDVMLPGRVGDTPLIGCGV
                     YADNDAGAVSMTGLGESIIRVAVAKEIADGLATGLSPVAAANRVLRKVVERVQGAAGV
                     LVLSPDGRCTIRHSTPHMIAGIIGPDGRAIVRDRFA"
     gene            complement(10136..10702)
                     /locus_tag="PK589_05625"
     CDS             complement(10136..10702)
                     /locus_tag="PK589_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19110.1"
                     /translation="MGSPVSRSHPLRQLFGALTEKSFTEHLGWPDLNVTEYVSNLLVE
                     FAHTDQLYKIQNTQGRPLDSVVEMLFESEVLLEAQSFERERDVHRHIGDFTLFMTGLF
                     PEYLRRLKSVGRIYHKDFLVDYVKAGKRSYGLVAEFGHYDPEQDTPLFRKLSENFELC
                     VTGLGFVRSDLDRMQDPACRRVKDLLLN"
     gene            complement(10801..11271)
                     /gene="dtd"
                     /locus_tag="PK589_05630"
     CDS             complement(10801..11271)
                     /gene="dtd"
                     /locus_tag="PK589_05630"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNJ19111.1"
                     /translation="MKAVIQRVTQASVEVEGQVVGRIGLGLLVLLGVAKGDGEGDLSY
                     LVEKVQALRIFNDDQGKMNRSLRDVGGALLVVSQFTLLGDTAKGHRPGFDLAAPPEAA
                     RAWYDEAVQRLRHEGLKVETGVFGAHMQVELLNDGPVTFIVDSRRPGAHADLKS"
     gene            complement(11298..11798)
                     /locus_tag="PK589_05635"
     CDS             complement(11298..11798)
                     /locus_tag="PK589_05635"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNJ19112.1"
                     /translation="MTETRTFKTSWGWMTVTASAKGVTSIDLFPSVRTVRHDSHRDEA
                     AASIVKEAQAQLLSYLDGERREFALPIDWSAGTSFQRKVWKAIAGIPYGRVRSYQWVA
                     MRVGGKQYARAVGMALGANPVPIVVPCHRIIAHDGSLGGFSSGLPLKRRLLGLEGTLT
                     QLRNAR"
     gene            complement(11800..13428)
                     /gene="murJ"
                     /locus_tag="PK589_05640"
     CDS             complement(11800..13428)
                     /gene="murJ"
                     /locus_tag="PK589_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNJ19113.1"
                     /translation="MSDPAATPEPSQPAVDETHSVVKAAGLIGIATFSSRILGFVRDM
                     VLARLFGATPAADAFFVAYRVPNLLRELFAEGSMSAAFIPVFTEYHTLKAKRDAWELA
                     SATFTTLLTIVTAVTLVGILAAPGIVWLLAPGFRESVDKLALTTLLTQLMFPYLIFIS
                     LAALAMGILNSLRDFAAPAFSPVFFNIFTIACMLFLSPWLSEPILAVAIGIVVGGVAQ
                     FAMQLPGLKRRGMLFGLRFNPGHPGVKRIGLLMVPSLLGLSVTQINITVSTILASYFS
                     GGPTYLFYGMRLIQFPLGIFGVALATAILPTLSAQAARGALDELRTTIGFGLRMIFFI
                     ILPAMAGLILLRYPIVHLVFEHGSFTKTDTLATATALLCYAVGLWAFAGVRIIVSAFY
                     SLQDTRTPAITAGIAVGANIGLSLWLMTILDAAGLALATALASMLNGSILVAVLHRRL
                     GRVDWGAVVRSAGRVLAASIPMAGICLWVASAPLWMETGQWIVKSVMLFGGIGFSVIA
                     YLGMHLMLGSDELDVVMGLVKRKLGRVARKFGVA"
     gene            complement(13486..14148)
                     /locus_tag="PK589_05645"
     CDS             complement(13486..14148)
                     /locus_tag="PK589_05645"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNJ19114.1"
                     /translation="MATGSKPALTEAERARVEAAVQAAEQHTSAEIVPLIVGRSGLYR
                     EAHHRAGLLSAVLALTVLVTIETAWLPWGWQASNAVWLVGITVLAYSVGVWVGTWPML
                     LRLFTSRDRMRHKVHMRAERAFAQQGLAQTRERTGLLLMVSLLERQVYILPDQALQGR
                     ISVDQWQDIVSVVVERMKVGDLAGGLCHGIEAAGVLLARACPSREGDNPNELPNAVIQ
                     DL"
     gene            complement(14135..15040)
                     /locus_tag="PK589_05650"
     CDS             complement(14135..15040)
                     /locus_tag="PK589_05650"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNJ19115.1"
                     /translation="MRLMGRAGWAVLAGWLWFTLVPSLPARALDVPPLSGRVVDLAHV
                     LPPATTAQLSETLQAHESKTSNQVAVLIVPSLEDEPLFDFSHRVATTWKLGAKGTDNG
                     VLLLIAIKERKIRLEVGYGLEGVLTDARSAQIIRNEIAPKFRAGDVPAGVTAGVQAVL
                     HTIEGTYRAPESPTAVHSDGEVIGNTIVAVIVGVVIGLFFSRAHRVLGPVAGGGLSFL
                     VAPWVIPALIAGAASVALVSLMSGLGQASGGGRRSRSGPFDDTWFSTRHGGWGSGGLG
                     DSFGGSGGFSGGGGDFGGGGASGDW"
     gene            complement(15071..15670)
                     /locus_tag="PK589_05655"
     CDS             complement(15071..15670)
                     /locus_tag="PK589_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNJ19116.1"
                     /translation="MISMARLMGASFLLAGLLALSGCGYNDLQGLDEDTKATWSEVIN
                     QYQRRADLIPNLVNTVKGYAAHEKDTLEGVVQARAKATSVQITPELLKDEAAFKRFQE
                     AQQSLSSALGRLLALAENYPNLKADQGFRDLQSQLEGTENRITVARKRYIDKVAEFNK
                     MVRYFPTNLTAKFLLHLDEKANFTVADEKAVAKPPEVKF"
     gene            15835..16140
                     /locus_tag="PK589_05660"
     CDS             15835..16140
                     /locus_tag="PK589_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19117.1"
                     /translation="MALDRSTDTPGGFQVRHRALGIFQGSSIGLAFWHPSSHMPEYGL
                     CRFATRAKAQDYVDFLSSPACPEPLSQADLIIEDFDHAEHERLTTEYPQASAWETPL"
     gene            complement(16222..16722)
                     /locus_tag="PK589_05665"
     CDS             complement(16222..16722)
                     /locus_tag="PK589_05665"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ19118.1"
                     /translation="MAQPLTCPQCGQDKVFRSPCSTLMERLQSLACMSPFHCQSCSFR
                     FSASRFGYRYPARMIDRREHLRIPVRLFLSFSGGKVRGEGTVRDISMGGCIIESQASV
                     HEDDIFYLQIVLENEQAPIEVAAMVRSVSSRGIAFKFLRSAQENKRLLAFVQSKTDSV
                     GPRSPA"
     gene            17227..17406
                     /locus_tag="PK589_05670"
     CDS             17227..17406
                     /locus_tag="PK589_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19119.1"
                     /translation="MTCGRCKGLMVDEWRPDFSPETFVWRCINCGSIVDPLIEQNRRT
                     RMAEQLLLEPVEVLQ"
     gene            complement(17475..18248)
                     /gene="xth"
                     /locus_tag="PK589_05675"
     CDS             complement(17475..18248)
                     /gene="xth"
                     /locus_tag="PK589_05675"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNJ19120.1"
                     /translation="MKVATFNVNSLRKRLPIVLQWLERHQPDVLCLQETKVQDSEFPL
                     AALATSGYEITFRGMKAYNGVAVLSRTKPEFVCYGFDDGGEAEDARLLRVVIQGIPII
                     NTYVPQGFEIDSPKYQYKLGWYDRLLKHFDMHLSPKEPAIWCGDMNVAPRPIDVHSPE
                     KHLKHVCYHEDARKAYGKTIAWGFEDVFCKLYPDRQQFTFFDYRAPSALAANKGWRID
                     HILATAPLAQKCQRVDVDLEPRRATDASDHTVLWADFDM"
     gene            complement(18377..18781)
                     /locus_tag="PK589_05680"
     CDS             complement(18377..18781)
                     /locus_tag="PK589_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19121.1"
                     /translation="MRRSIAVMVAAAFVGMSSFAAADDLGMGKMSEGMKQDMGTFSDE
                     MNAHKEELKSDLKAKKESAKSELKAKRDEAKAKKKQAKADAKAKKEHVKAKVKAKKDA
                     ARAKAADMKSAADDTVQEMGGMKNDLKEATRY"
     gene            complement(18975..19799)
                     /locus_tag="PK589_05685"
     CDS             complement(18975..19799)
                     /locus_tag="PK589_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19122.1"
                     /translation="MQDMLPVIGSSVFFDLLKSILLLLILLIARTVLVRWIKNNQALT
                     IDARRRWIVTTRNSMVLCLFVGLVVIWAHELEAFAVSLVALAATLVLATKELILCWSG
                     AALRVGGGVYGVGDRIQLGTYRGVVLEYDVFATKLLEIGPGQTSHLYTGRTVVFPNSL
                     LFGNPLIKESPSQEYGLYVLSVPLLSTDNWQAAEQALLGAAKVECAPFMDQMGRQMKL
                     LEQRNLLEAPSPEPRITIQLPEAGRIHLVLRFPAPDRGRSRVEQAILRRYLTTIRQ"
     gene            complement(19817..20308)
                     /locus_tag="PK589_05690"
     CDS             complement(19817..20308)
                     /locus_tag="PK589_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19123.1"
                     /translation="MTRNDNRLSYVSNRLLPLSVRWSRLGMLLCLAMLSGCGGPWRDT
                     YLNKGVHKLTQADIEDKFGPPSTAKTPVLGGDTVWTYRVPMGDREVHPWDPSSLAAGK
                     KTKAPPSPSPFGKSLAGNDEPYREPLYCYRYTLSFDEQKRLKDWKREECVPRTSEDGT
                     VVQ"
     gene            complement(20292..20507)
                     /locus_tag="PK589_05695"
     CDS             complement(20292..20507)
                     /locus_tag="PK589_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19124.1"
                     /translation="MSDLRTNALIARGMGIVFIAGGFMLGIYGLEFPNTMWLQTALGL
                     LVTGMMAQGYAMYCSIRWVREQRDSER"
     gene            complement(20579..21262)
                     /locus_tag="PK589_05700"
     CDS             complement(20579..21262)
                     /locus_tag="PK589_05700"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ19125.1"
                     /translation="MRRPALVGLCALLLFMAWSCGQQTWEAAMQAGEVAVQRGQLGEA
                     EQIYAAALKKAEEFGPHDRRVAVTLAHLAQVYSTQGKFVEAEPVYLEALKIYQEVHGE
                     NHLDVAAMLNNLGVLHRKHGQFADAQRLLTRALAIKEKLLGPDHLEVALAVSNLAAMY
                     AAQGDTAQAAALFARALAIREKQLGPDHPDVAKSVIDYAGALRKIGRTPEADALEGRA
                     AAIRSKTKS"
     gene            complement(21259..22668)
                     /locus_tag="PK589_05705"
     CDS             complement(21259..22668)
                     /locus_tag="PK589_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ19126.1"
                     /translation="MPSQLSQKNERRHDVVVIGGGSAGYAAARTACEAGADVAIVDQG
                     PLGGLCILRGCMPTKTILRSAEIMALMKRAREFGLAPVTARADLSAIVDRKNRLVQEF
                     ADYRIAALRDPRFTLYESHAEFVSPHEICAGGLRIRGDAFVIATGSSVAKVAIPGLSE
                     AGYVTSDEMLDVRHAPVSLAVLGGGLVAVEFAQFFSRIGTKVTMLQRGATLLSDMDQD
                     VGQALASAFEDEGIEVLTGVAFDQVTSSPTGKTVHFRQNGVSHSRSAEVIFHALGRCP
                     NLKGLNLEAAGVKVIDGRLVVGADMRTSQPHIFAVGDVNDLTPIVHLAIQQGETAAFN
                     ATHPDVPARVIDHRLDSEMVFTDPQVAIAGLSERQCREQAIPYLTASYPFADHGKAMC
                     LGALAGFVKLLCRPDNGELIGAQIVGPEAAELIHELIAVMYFHGTAQDLLRIPHYHPT
                     LAEIVTYPAESILEQMGRI"
     gene            22718..22936
                     /locus_tag="PK589_05710"
     CDS             22718..22936
                     /locus_tag="PK589_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HNJ19127.1"
                     /translation="MIKPGRYRHYKGNDYEVLGVARHSETEEEYVVYRQLYGTGGLWI
                     RPMTMFSESVSIGGEIVPRFRRLEEGPA"
     gene            complement(22916..23389)
                     /locus_tag="PK589_05715"
     CDS             complement(22916..23389)
                     /locus_tag="PK589_05715"
                     /inference="COORDINATES: protein motif:HMM:NF020254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HNJ19128.1"
                     /translation="MDDSNQVWRRLEIVEWSQWLLDSYRHCVGRELMERVDGAEAQAQ
                     ALFTAPIVVVSHGMQDDPILNYGSQLALTLWEMTWEQLVHMPSRLTAEPVNRTERESM
                     LERARTQGYIDNYRGVRISSSGRRFLIEQALVWNVVDRAGYRRGQAATFSRWTFL"
     gene            complement(23480..>23763)
                     /locus_tag="PK589_05720"
     CDS             complement(23480..>23763)
                     /locus_tag="PK589_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19129.1"
                     /translation="KSAHSRTPMLDSPDRILEGVPLDLTYQVGQGGRVVLKTKGQDAP
                     TEVWSCSVVGHDSRAKNDGTALRRGDLVMTLLGSHGQALFVRHLRKTPA"
     assembly_gap    23764..23863
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23864..24186)
                     /locus_tag="PK589_05725"
     CDS             complement(<23864..24186)
                     /locus_tag="PK589_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19130.1"
                     /translation="MARWQWALLVACCSVMLASGSALASDRAHAKSLTRSGLTVPASS
                     LRSCSGEDLLGTWSLVAFDSAYRFKDPQAPYLFPYQVFQYSREQGAKSAHSRTPMLDS
                     PDRIL"
     gene            24605..26008
                     /gene="leuC"
                     /locus_tag="PK589_05730"
     CDS             24605..26008
                     /gene="leuC"
                     /locus_tag="PK589_05730"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNJ19131.1"
                     /translation="MAAQTLFEKIWNDHVVRAEPDGTTLLYIDRHLVHEVTSPQAFEG
                     LTVAGRTPRRSAAALAVPDHNVPTTDRRVAIADPVSAKQIQTLDDNCKTFGITLFGMN
                     DIRQGVVHVIGPEQGFTLPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLV
                     QKRPKTMEVRVDGQLSPRCSAKDIILAIIGKIGTAGGTGYVIEYTGSAIRALSMEGRM
                     TLCNMSIEGGARAGMVAPDETTFNYIKGRPMAPKGALWDQAVTAWRNLHTDAGAKYDA
                     VVELRAETIAPQVTWGTSPGMVTGVDGTVPDPRSMSDDKLRQATERAIEYMALKPGMP
                     IRDIKIDKVFIGSCTNSRIEDLRLAASFAKGKKVAGSVHAMVVPGSGLVKQQAEQEGL
                     DRIFKESGFEWREAGCSMCLAMNADVLQPGERCASTSNRNFEGRQGAGGRTHLVSPAM
                     AVAAAIEGHFVDIRQWS"
     gene            26087..26707
                     /gene="leuD"
                     /locus_tag="PK589_05735"
     CDS             26087..26707
                     /gene="leuD"
                     /locus_tag="PK589_05735"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNJ19132.1"
                     /translation="MDAFTTLTGLVAPLDRLNVDTDQIIPKQFLKTIKRTGLREGLFY
                     DWRRRKDGSQDPEFFLNQPRYQQASILLTRDNFGCGSSREHAPWALLDQGFRCILAPS
                     FADIFYNNCFQNGILPVVLKAAEIQALFEGVAAHDGYTLTVDLAAQQVNTPKGMAYHF
                     DIDPFRKDCLYRGLDAIGLTLQHAAQIGEYEQRRRTQAPWLFQDVR"
     gene            26727..27116
                     /locus_tag="PK589_05740"
     CDS             26727..27116
                     /locus_tag="PK589_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19133.1"
                     /translation="MTWWETLLFPVAWLWRPKLLFTLLFFGGAAYLLIVFNWSYSDGD
                     RAGYLQKLSRKGWFCKTQEGELAMTTVPGVAPVLWSFTVWDENVAKKLDGQMGKRVVL
                     HYKEFRYIPTTCFGETPYFVDRVEVLD"
     gene            complement(27126..28184)
                     /gene="corA"
                     /locus_tag="PK589_05745"
     CDS             complement(27126..28184)
                     /gene="corA"
                     /locus_tag="PK589_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNJ19134.1"
                     /translation="MKLKHKHSRKAGLPPGTLVHIGEKRSETVTITVYEYGEGQYQER
                     TVSKPDEVVATGEPTVRWVDVGGIHKMEVLEAFGKMFGLHPLLLEDIANTDQRPKLDD
                     YGTYGYLVLKMLYEGQSEGDINVEQVSLVFGENFVLSFQENGGDVFQGVKERLRNGKG
                     RLRHAGADYLLYALMDSIVDRYFVILETLGEKIEALQDLCVTNPGPETLRDIHALKRQ
                     LLFFRRSVWPLRDVMNNLSRSDGQFLQGSTKVFFRDVYDHIIQIGDTIETLREMVSSM
                     MEVYLSSVSYRLNGVMKVLTIITTIFMPLSFIASIYGMNFEHMPELKSPWGYPAVLGV
                     MALTGLAMLYFFKQKKWL"
     gene            28320..29129
                     /locus_tag="PK589_05750"
     CDS             28320..29129
                     /locus_tag="PK589_05750"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNJ19135.1"
                     /translation="MTMEYELTILSQAMSEAGREALRLASAGFETFTKADQSPVTSAD
                     LAVNRILQDRLSTAFPEDGWLSEETTDNDTRLSRKRVWIVDPIDGTRSFVRGLPEFCL
                     SVALVENGVPTVAAIFNPATGEFFSAIRGRGLRVERLSDSDQPGFTSSERPVALVNPW
                     ELRVGRFEGLQDHLHCRPIGSIAYALALVAAGQADAAITLAGGNEWDVAAGVLLVEES
                     GGRATTAAGHPVTFNRPDPRLPGTLAVGSDLPAPVRTRLIQHGLAAASHPL"
     gene            29160..30068
                     /locus_tag="PK589_05755"
     CDS             29160..30068
                     /locus_tag="PK589_05755"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ19136.1"
                     /translation="MAALATSERYSMNVALLGTGLLGQGVAERLHATGHDLTVYNRSI
                     KKTQALRQQGIRIALTAAEAMSAADVVLLLLSDAAAIRAVLFDPAASRAIDGRTIIQM
                     GTIGPAESCSLEKDVTGLGGRYLEAPVLGSIAEAKAGTLLVMVGATPEHYDKWAPLLR
                     YLGEEVRLIGPVGKAAVLKLALNQLIAAEMAAFAFSLGLIRRGGVDVNDFMGVVRKSA
                     LYAPMFEKKLSRLEKRNYAQPNFSTRHLLKDVELALSAAEKARLSPAGLQGVRSLLID
                     TIAAGLGDVDYSAIYEQIDPLGSEHG"
     gene            complement(30072..30557)
                     /locus_tag="PK589_05760"
     CDS             complement(30072..30557)
                     /locus_tag="PK589_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19137.1"
                     /translation="MNRMKQNQWFFLMMGLSLLSIQACSHAPRPQETTWTEPPAGEIS
                     LPRGEHAVLAGEWEYEEGGMVVPLRLDRFGNGSYDFKDGRFRTDLFSDHRWAGAWAQR
                     ENDREGGFEITLSPDYSEGEGRWWYTRIEGDKAPTKAGGRFHVMKVQSIVDNQPWPSA
                     R"
     gene            30900..31358
                     /locus_tag="PK589_05765"
     CDS             30900..31358
                     /locus_tag="PK589_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ19138.1"
                     /translation="MPSSDNKHAGGEAQNPSHSSPLNILLVEDMEDNRVLVSLLLKAF
                     PYRLDLAEHGAAGVEKFQSGRYDLVLMDIQMPVMDGYAAIRLMRQWEKEQQRSETPIL
                     ALTGNTHDEDIGKAQAAGFTAHVMKPLKKVTLLEAIQRYAAPFSRKESAA"
     gene            31355..31690
                     /locus_tag="PK589_05770"
     CDS             31355..31690
                     /locus_tag="PK589_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNJ19139.1"
                     /translation="MSDMAPVTSSCGAGPIVVLVDSAFEPLIPKFMANRNKELATMTE
                     ALATSDFATIRQLAHGMKGVSGSYGFHAMTTIAGRMEEAAKVANGTSIRQDLLELASY
                     LTRVEITYE"
     gene            31899..32294
                     /locus_tag="PK589_05775"
     CDS             31899..32294
                     /locus_tag="PK589_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ19140.1"
                     /translation="MDNPETPKLRVLMVEDEEDTASLLKFLLERANYQVVHAKDGRQA
                     QELVNTIAPPDIVLLDVMLPFLSGLQVLTVIRKREGWKKVPIVMLTADGSEHDIKRAL
                     ESGANDYMIKPFNPRELTSRLKRFVAHAA"
     gene            32316..33407
                     /locus_tag="PK589_05780"
     CDS             32316..33407
                     /locus_tag="PK589_05780"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ19141.1"
                     /translation="MLGLDQSDLTLRIGTIAAISIYSSVLLLLLGILLLRYIRNNKDR
                     RRRAVTEIWRPILAECVVEVPETLPPLTTRDHLVLLYLWNHCYESIRGDARENLKELA
                     RRTGTDRFAKELLHARLLRRRLLAIVTLGHLQERSIWNELAAALQADNAFVSLVAAKA
                     LLHIDAKAAVPLIIPVISQRKDWSPLKVVAIFEPAGADIAAEAIAKAACLASPEIGSR
                     LIRHLAATQSQHALPPLRDMLQKGTPSDDLLAACLFLFGECSDPRDIVTIRTHLSHPT
                     WYVRLQAASALGKVGIEEDEARLIALLDDEHWWVRYRAAEALSNLPWMTTERLTRVHN
                     TLTTVESQEHLVPFMAQSNAKAQHPARIA"
     gene            complement(33435..35390)
                     /locus_tag="PK589_05785"
     CDS             complement(33435..35390)
                     /locus_tag="PK589_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19142.1"
                     /translation="MQRSTLVRVSGIALLFVSLVIGVRILVSAPPVQHMEVPEKDFHR
                     FQGVAGPDDATVVPRHVPTAWTTYEPGSTSRLAILLTDPDSSWLGLAHGLKSIGIPFR
                     MTRNVHEALAHKVVLVYPMISGKVLKPEELQALAEFPKTGGTLIGVQVLGGGLNHVFG
                     FRDAVASRQRYDMTLATADPLLADLTDPREQRLRVGIRDKGIEVMGTYGYTDASAPLA
                     RFDDGTAAVTQVAHGPGRAYAIGVDLGFLLLKGYNNRGEELVKTFDNEFDPTLDVWLR
                     LIKAMYRTAQEEAVTLGTVPFGKSLSVMMTHDVDFTQSIKHAVEYAEYEKRQGVVGTY
                     FIQVKYIRDYNDDIFFNDEGVRYVKQLADLGMELGSHTIAHSRVFNQFPMGSGRESYP
                     SYAPFVKDRTTAYNVSILGELRVSKFLIDHFSGQSIVSFRPGELSNPFGLPQALLATG
                     YRYSSTATANNSLTHLPYQLNYDRLTDSEVEIFEFPVTIEDEALPKMGDRLPQALEVA
                     KRISRYGGSVVVLIHPNVLDHKLAFEKGFVEGVRTYAWFGSVGEFGRWWAARNRVEVD
                     VVRDADAMRVTLNVPVSLAGLTLEVPPGWEVARSGNPLRGVAQHGTMVTFPQLQGTYT
                     VLFTPSSLTPSASRTSSSRSNTVNMGL"
     gene            complement(35395..37758)
                     /locus_tag="PK589_05790"
     CDS             complement(35395..37758)
                     /locus_tag="PK589_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ19143.1"
                     /translation="MNDSFTWINQRTSVAGLLAVIALAAAWFFAPATMIAAMAPELSP
                     RQTLQQARVLEDEKRYEEAIAAYRQYLQAKPDDDDVRASVAKLLSWQGQWDAAAAAYR
                     DLLTRHPLDHDIRVGLAQVLSWQKHYDLAREEYERVLHDEPDHVEALTGLGNVYLWSG
                     HQDQAIPYYERVVALTGDADVAARLQSLKAASRSPVVPVNPSGAADGNDSLTAHAAQT
                     LERGRRLETMRQYADAVTVYREELQLSPTNDEVRAALARVLSWQGVHPEATTLYREIL
                     ARHPQDQDIRVALGQVLSWQKQFEEAGRVYQDVLQSDPTHVEARRGLADVAHWQGHYA
                     EAVQRYEALVAETHDPELEAQLKTIRSEWRTTSPQVAEHPASTHDSQGTDERVIGAAV
                     EEATKFEVAKQYPEAERVYRDVLRRHPDNDDIRTALARVLSWQGAHAEATTLYREVLA
                     RHPEDQDIRLALAQVLSWQKQFDEAERLYGAVSQAEPTRIEARRGLAEVAHWRGDRSE
                     ALQRYEALLDDTHDPAIEQQIKAVKSELLVSPRAAVGQGLAGLRLPYRDYAKIGYGHY
                     TYTKNQPDERDLLFELAKPLGNQTLVMRVEPINRFGFHDTPMSAELYSPLWSRAWGYI
                     AGQATINPHFSPNYSFVGEVAQGLGGLHSSLAPFEASFGYRRLNYKHDDIDLLMPGLT
                     IFLPFNLWLTEKVYFIPNTGAVTLASQLTWRPADRLQFFASGSFGTSGERIVAAQDFT
                     RVGSRTIQGGVTFPINERFSAETSGYYEDRGFLYVRRGGNFNLIYHW"
     gene            complement(37792..39468)
                     /locus_tag="PK589_05795"
     CDS             complement(37792..39468)
                     /locus_tag="PK589_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19144.1"
                     /translation="MSVSTHIDQCVSARDGVARDQIDGKSVGRGTEAVGRRLFATVLC
                     SAAVISILWLSLAQAEDIPLSPPIPSTIHEIELTYAGNSTDPAVAFSGPVPPAGPLPY
                     RPFLPILELLPGFGVAHVFGPCHGQAVRSDSHLELEWTAGDAGRCGQRIELRPAGRPM
                     DLMSYQMLRLRGRATGQVVVAIEDLAGHQREHNQSLATVTGTFDLTIPLKEIGRGVDL
                     RYVTALVVSAEGPRGRIEFEQLTVSQRNTVKKPPGGTGFWVWNYRAAIQEPQPLLATC
                     RRQACSRMLIQMPSLNDDEAIWEGYASLMAQVQEAGIDALALDGYPEAIQEPHLLADK
                     VQRLFHLVRGGAVSGIQLDIEPYLLPGFLDDDGQLRRYLGAIETVSEAIHGRAKLSMV
                     IPFWLATPTVGGRPLAYAVMDRADEVAVMSYRTDLEEVQDIAEDILRYGDVSEKPVWL
                     AVETTVLPLEEHVVLRRDLRPDHADALLDREHKRLRWKPISEADRLTGSGEWFRIHRR
                     FTVRPERLTFAGRSRVEVSQAISHMLDTTMHPSFAGVIIHDLDGFRALPE"
     gene            39449..40855
                     /locus_tag="PK589_05800"
     CDS             39449..40855
                     /locus_tag="PK589_05800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ19145.1"
                     /translation="MWVDTLISTSEWLFLLYFLGLTAGYLMLDLIAIVHLRQYLQERV
                     LESLPHSYTGFEPQISILVPAYNEAATIAGSIRSLLQLSYSEYEIVVINDGSKDATID
                     VLRKEFDLFPFPEACNTQLPTAEIRGIYHSSSHTNLRVIDKENGGKADSLNAGINLSI
                     YPLFCCIDADSILQPDSLRRAVQPFLDDHRTVASGGTVRIANGCRVENGFLTKIGLPS
                     NLLALFQIVEYLRAFLFGRLGWSPLNAMLIISGAFGIFRKEAVVTVGGYRSDTIGEDM
                     ELVVRLHRHLRMGDKPYRITFVPDSVCWTEAPEDLRTLKNQRIRWQRGLCESLTKNLD
                     LLCHPKGGTIGWLAFPFMAVFEWIGPAIEVFGYCFLIFSFVMGFVSIQAWLILLVAAI
                     GLGVLLSVSALLLEEMSFHVYPDQRDVLKLLLAAILENFGFRQLNSLWKLIGLYRWVC
                     GKKATWGTMTRTASWQAR"
     gene            complement(40879..42033)
                     /locus_tag="PK589_05805"
     CDS             complement(40879..42033)
                     /locus_tag="PK589_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNJ19146.1"
                     /translation="MKKSDRTARLAQSDIRAMTLACAKVNGINMSQGVCDTPVPPVVV
                     QGAQQAMSQGHNIYARFDGIAELRQAIADKVASFNRITADPETNVTVSAGATGSFQAT
                     CMALLNPGDEVILFEPFYAYHVQAILAVEGVPRYVTMRPPDWTMDVKSLEQAVTSKTK
                     AIVVNTPGNPSGKVFTRRELEQIAEIACRRDLSVITDEIYEYFVFDGREHVSMASLPG
                     MADRTITIGGYSKTFSITGWRIGYSVAEASWAKAIGAMSDVLYVCAPTPLQYGVAAGI
                     RALGPSYYKELALDHQRKRDRFCGALAKAGLPPAVPQGAYYVLADVSRLPGNTSRERA
                     MYLLDKTGVAGVPGEAFFEGTEGSRFMRFCMAKTDADLERASQAIERLTS"
     gene            complement(42194..42775)
                     /locus_tag="PK589_05810"
     CDS             complement(42194..42775)
                     /locus_tag="PK589_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNJ19147.1"
                     /translation="MARALFPLAVAGCLMALMPPASRLAFDPIMKEVAGRPTAEPIAS
                     LIATYAEQYALDPALLQAVIKVESNFNSDAVSSKGAIGLMQLMPLTAAAFHVLDPFDP
                     KDNIRAGAALLRGLLDRFGGDLSLALAAYHLGEARVRQAIGVPALPATQLYVNRVLGH
                     YDRFRAGTSRLPTRVTTRREERRTLWSLQQAPE"
     gene            complement(42843..43229)
                     /locus_tag="PK589_05815"
     CDS             complement(42843..43229)
                     /locus_tag="PK589_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19148.1"
                     /translation="MMRLLADCRSLMLVLPLMVTATGCAESALQGHSPQEDLGMGIVS
                     GTLGAQSRSAVSSGEQSTASVVGQVQAVEGGAYLIRDVRGQEHRIPHDENTRIDRPAH
                     VGDRIQSWFDRHGRAVLIRSLDEEGR"
     gene            complement(43445..43822)
                     /locus_tag="PK589_05820"
     CDS             complement(43445..43822)
                     /locus_tag="PK589_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNJ19149.1"
                     /translation="MRDCRTIAVVGLSSNPARPSYRVASYMQQQGKVVIPVNPRETDV
                     LGERAYPSLSDVPGSIDLVNVFRRSEEAGAVVDEAIRIQAKAVWLQEGVVDEAAALRA
                     RQAGLQVVMDRCWLKEYVRCVGE"
     gene            complement(43861..44481)
                     /gene="msrA"
                     /locus_tag="PK589_05825"
     CDS             complement(43861..44481)
                     /gene="msrA"
                     /locus_tag="PK589_05825"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNJ19150.1"
                     /translation="MRSIIGRFGALGVLPMVLAGLLVSQSQAADPSGAAKAYFAGGCF
                     WCMEEVFEKVPGVTAVVSGYMGGRVEHPSYEQVSAGGTGHAESVEVVYDPTKASYTTL
                     LDAFWHNVDPVTPNAQFCDHGSQYRSVIFYQGEEQKRLAEESKRAIEQSQRLTQPIVT
                     ELTKASQFYPAEEYHQDFYKKNPIRYKFYKFNCGRAQRLEEVWGAP"
     assembly_gap    44624..44723
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<44724..45229)
                     /locus_tag="PK589_05830"
     CDS             complement(<44724..45229)
                     /locus_tag="PK589_05830"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19151.1"
                     /translation="MRYIVGVMGPAKARKKDVDNARVLGELIARREWVVLTGGRDVGV
                     MDAACQGAKKVPGSLTIGVLPSARERVSKYVDLAIITEMGNARNNVNVMSSNVVVVCG
                     LLGAGTVSEVALALKAGKPVILVGATPTEEKFFKKLGGRLIASVDSPEEAITLMMKQF
                     EQQQPDLV"
     gene            complement(45232..46053)
                     /locus_tag="PK589_05835"
     CDS             complement(45232..46053)
                     /locus_tag="PK589_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ19152.1"
                     /translation="MLKTDYVFQATEEARELQRLQMLERIFDAGTQRRMLATGLTTGW
                     HCLEVGAGAGSIVRWLEQRVGPSGKVVALDTNPRFLRGSSTSTIDILQGDICDTDLPL
                     AMFDLVHARYVMIHIADYQKAFERMLRCVKPGGWVVIEEPDFEAARAVTASDDARTSF
                     TRVTAAIERMFTSLGMDYALGAKLPALFQRQDLSHLTVEHEGHLSAGGGATAQLMKLS
                     AEQLREKYVATGLVTESDIEQYCRLADDPDSWAIYYATVAVTGWWQPQCGGRTQG"
     gene            complement(46110..47744)
                     /locus_tag="PK589_05840"
     CDS             complement(46110..47744)
                     /locus_tag="PK589_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19153.1"
                     /translation="MKNARYVRGVVYGLVIAGLALPVSDGIARSEVPLFENLGVLQHK
                     ITTHSDLAQKYFDQGLRLVYAFNHEEALAAFTEASRLDPDAPMPYWGVALSLGPNINA
                     GMDSKAESRAVEAVQKATARLAHATPTERAYVEALAARYSVKKAATRKGKDEAYAKAM
                     RRLAHEYPEDADAATIFAEALMVLRPWDYWRADGRPQPGTEEIVTTLEAVLQHQPDHP
                     GACHYYIHAVEASPDPQRGLPCAMRLPSLMPGAGHLVHMPAHIYMRVGRYRDASERNA
                     KAATVDHEYLLQHPLEGNYASGYYAHNLHFLNASLVMEGRSAEALQAARDLLSKISVN
                     EIVKEPSLEWYAPTVLLTMARFGQWGELIRQPPPPKELRYTTGLWHYVRGLAFAATTR
                     FGSAEGELSNLRKSLKGLARAKTAEGKISRTLLKIAERVLAGEIAARKGDFDAGIQAL
                     REALQLEATVPYSEPPFWFQPVRHNLGAVLLLAGRPGDAESVYREDLRLNPENGWALH
                     GLVHSLQAQRKETAQESARLHTAWAQADVPLTGSRF"
     gene            complement(48169..48876)
                     /locus_tag="PK589_05845"
     CDS             complement(48169..48876)
                     /locus_tag="PK589_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19154.1"
                     /translation="MTRKQQRLAEKPIHLLTLAPDKGEKGAVNPFAPSPLRAAIGKVF
                     VKLEDMTERFEDWCQYGNSEERTEQPLGQRVSNWLGAPVARHIEHQVQAEHGLVPARR
                     WDGAVGDLIFRLRDRAGFVQRALTAQARELKDWVIQHTQSTQAELHDLREQVATQQVQ
                     MEDLSAQLHDLRALVKSQQQVLMYMGKDMEMAPPAELELSLVSPRSIPYRERDKKSSR
                     DRRDTSNEASQIPYLNA"
     gene            complement(49029..50375)
                     /locus_tag="PK589_05850"
     CDS             complement(49029..50375)
                     /locus_tag="PK589_05850"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNJ19155.1"
                     /translation="MTSSITSSWPKLTSRQEFEFLADLVMSHSTGDHTFFSLRDSHGG
                     TTRFANNQIIQNVNLRRGHLSVTVAFGRQHGTASTTDFTAGAVREALKQAESLARLSP
                     DDPEYLPPVGAQSYLSLPTSRPETTAAGPARRLDYAREAIGQCKMENLNAAGTVSSST
                     TTVGLAAETGLRAYEERTEGRFSLTVQAGDATGWSAAAHRSIDRLHVQERTLAAIIKA
                     KRGADEPQELPPGRYTVILEPAAVAGLLSWMIWMLDAKSFYKGTSPFSGKLGSRIIDR
                     RLSLVNQPAHADLLGNSFTHEGLPVIESGWIESGVLTQLLHDRYTAREHQIEPLTTLE
                     SPHLSGERPIGTRVDDLIRTTQRGILVTNFWYIRPVNPSDLTLTGMTRDGTFLIENGE
                     ITSAVRNFRFHESPLRAFNQVETYTAPAEAVTSETGKALVPAMRIHDFHFSSVTKF"
     gene            complement(50372..51835)
                     /locus_tag="PK589_05855"
     CDS             complement(50372..51835)
                     /locus_tag="PK589_05855"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNJ19156.1"
                     /translation="MSGSQWDELAELALSRVSSAGVEYADIRLLDTTTRTISGEDRRI
                     AHIRESTDRGFGIRVLHRGAWGFAASSIISLEEIPRVADLAVEIAKGSASLAITKVHL
                     APEPVHRDRIVTPSRLDPFAVPLEEQTALLLSTMDIIQRHPGVVRSHASLWAQRDRKL
                     FVSTEGSHLEFDLLAMQGDCTATAVHNGRFASRSFNTPHLRTGYELVRDADWVREGAR
                     IAEQAVEKVRAPAIDAGRYDLVLDPEHLSLTMHESCGHPSELDRALGYEANYAGTSFL
                     TPDKRGTYRYGSADVNLVADNTEPGTLAATGYDDDGVSCQKWDIVREGIFVGYCTNRE
                     VAPRIKEERSRGSNRADSWGSVPMVRIANIGLEAGTSTLDDLLADVKRGIYIEGHGSY
                     SIDQRRYNFQFGGDAFWLIENGRRTHMVRDVIYHGITPEFWGRCDGVADATHRRRYGF
                     ITCGKGQPGQSGWMTHAASHARFRRVDVISGHTKADA"
     gene            51950..52261
                     /locus_tag="PK589_05860"
     CDS             51950..52261
                     /locus_tag="PK589_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19157.1"
                     /translation="MITPPQTPRQRIMTLLTGTLLSSHQLAQLVGMPERQVEDHLTHI
                     VKTLARDTSRTFLLEPSHCQDCTYTFRDRTRLTRPSRCPRCRSEAITSPRYGIREEAG
                     S"
     gene            52359..52580
                     /locus_tag="PK589_05865"
     CDS             52359..52580
                     /locus_tag="PK589_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19158.1"
                     /translation="MNTFLTWVILILLVSTSACGPDQASHMLETAQFEERQTNKAHAK
                     ELYEDIIRHYPDSPAAKTARTRLTELAKP"
     gene            complement(52821..53621)
                     /locus_tag="PK589_05870"
     CDS             complement(52821..53621)
                     /locus_tag="PK589_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNJ19159.1"
                     /translation="MTATDPLSLLMTAWAASALLMVALWLLERRLHNLALADVGWCYG
                     LALVVLWYTTAVPGEPARRLLVCGMIVLYAARLGTHVLIDRLWDKSEDGRYRALRVQW
                     GEQGPLRRFWYFQLQAAAIVWFSLPPLIVMQNPHPPFHLMELLGVLLWMAAVTGEAMA
                     DWQLASFRRQPWNRDRVCREGLWYYSRHPNYFFEWLHWWSYVVMGLASPLGNWALTLI
                     GPLTMGWALLKVTGIPWTEAQTMTSRGEEYATYRRTTNAFFPWFPRRL"
     gene            53799..54152
                     /locus_tag="PK589_05875"
     CDS             53799..54152
                     /locus_tag="PK589_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ19160.1"
                     /translation="MKGEHPVTMAIVMAVGTVLMQADVHGEAAGKADALAKGKQVFTR
                     HCAGCHGAEGKGDGYKLLGPDPANLTAPATRKQSDKALLTTIHEGKPNMPSWKGLLSE
                     RDITSVLAYIRSLPH"
     gene            complement(54165..55043)
                     /locus_tag="PK589_05880"
     CDS             complement(54165..55043)
                     /locus_tag="PK589_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ19161.1"
                     /translation="MKRIALITGAAGLIGGYLVRSASRWVPDWEVVGVTRAEADFTDR
                     AQVEQLWNRHRPALVVHCAALSRIGLCEQDPVRARLTNVEATRLLADLARDVPFIFLS
                     TDQVFDGAKGQYVETDAVHPLNVYAQTKAEAEQVVLANPAHTVVRIALTAGMSPTRDR
                     SFVEDMVRTASKGVTLTLFTDEFRCPIPAGALVRALWELGMQRRPGLYHLGGHERLSR
                     WEIGELVAMRFPELRSSIQPGSVADYQGPPRPPDLSMCSEKIQALLSFPLPGFRQWLT
                     EHPSESGDPWDYPAAR"
     gene            complement(55040..55714)
                     /locus_tag="PK589_05885"
     CDS             complement(55040..55714)
                     /locus_tag="PK589_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNJ19162.1"
                     /translation="MTRPTDKHRIFITGATGYVGARLIPLLLERGHDVTALVRESSAG
                     KPVVGCRVVVGNPLDAESFAESVRGHDTLVQLVGVPKPSPWKGPQFRAIDGPSAMAAI
                     HAAASTGVQHYVYVSVAHPAPIMRDYIAVRRDCEEALRRAGLVATILRPWYILGPGHW
                     WPLALLPVYRMLERVPATREAAMRLGLVTIREMVAALLWSVEQPPVKTRVIDVPEIRR
                     LGRSEP"
     gene            complement(55711..56967)
                     /locus_tag="PK589_05890"
     CDS             complement(55711..56967)
                     /locus_tag="PK589_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNJ19163.1"
                     /translation="MSMIWGRTLSRWLAVLALASASCTTIDAPRALPPDHRPAPTRAT
                     LQLGQKELADGRFVGLAFSGGGSRAAVFGAAVMKELERAGLLSQVDVLSAVSGGALPA
                     ASYALEGFGDFRFQDGFVEQIGRDIQGAVAGPWYAAPHNLVRYAFKDTIPAEPVIRAL
                     DDQLFRGATFADLNPSRPLLLLNSTDALTGDPLVIAEERFAPLGIPLAPFSIARAVYM
                     SAAYPGVLEPLKLTHGSTATNGASADPIIAYDGGAADNLGIRTLMQVVERALVNRPMT
                     DLFPQGCLVISIDATGRQRNETHTPLSAAAALLRGHRRNVLELAGIPASRQDVAQFGT
                     FRVGENGGGGSCRFWHVALRQLPDTDPLGDHVTHIKTNLGLSTEDQAALITAAARLVA
                     KGREELNGMAGWAGFLETKPALFSHP"
     gene            complement(57436..59118)
                     /gene="ettA"
                     /locus_tag="PK589_05895"
     CDS             complement(57436..59118)
                     /gene="ettA"
                     /locus_tag="PK589_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNJ19164.1"
                     /translation="MATNDKQVIFSLVGVGKVYPPKKQVLRDIYLGFYYGAKIGVLGL
                     NGSGKSSLLKIIAGVDPNYVGEITRSKGYSVGLLEQEPQLDPNKTVKEVVEEGKKELV
                     ALLHEYEAVSNSMGDASPDEMEKLIDKQAQLQEKIEAANGWELESELEIAMDALRCPP
                     ADQKVSVLSGGEKRRVALCRLMIQEPDILLLDEPTNHLDAESVQWLEQHLQQYKGTVI
                     AVTHDRYFLDNVAGWILELDRGHGIPFQGNYTSWLEQKQERLEKEEKAESKRKKTLEH
                     ELEWIRMSPKARQSKGKARLNRYEELVNQKQDQLAAELEIYIPPGPRLGDVVVEAKGI
                     SKAFGDNVLYENVEFSLPKGGIVGVIGPNGAGKTTMFRMIIGKEKPDSGTIRIGETVK
                     LGYVDQDRSLDPNKTVYEIISDGQDTVMLGKAEVNARGYCARFNFAGTDQQKKVKDLS
                     GGERNRVHLARMLKEGANLIILDEPTNDLDVNTLRALEEGLEGFAGCAVISSHDRWFL
                     DRIATHIMAFEGDSKVVWYEGNYSEYEADRKRRLGKEADQPHRIRYRKLTRN"
     gene            59218..>59735
                     /locus_tag="PK589_05900"
     CDS             59218..>59735
                     /locus_tag="PK589_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HNJ19165.1"
                     /translation="MIPDARIQRRSCRIILALLLPFLAWTGVASALADDREQRDLVEQ
                     SRMTLSNFLADSNMTWFRDHVPEAKGLFIVPQYMKGALIYGAAGGSGVFVAKDDKTGE
                     WSEPAFFTMGAASFGFQFGAQMSEVVLLVLTQRGVDSLLLGNFKLGADGSVAVGPVGA
                     GVSGATTPNLSA"
BASE COUNT        12512 a        18346 c        16895 g        11782 t
ORIGIN      
        1 tccatcggca actccaagag gtgacggacc ggttctttcc tgaaaccgag tggacaagta
       61 tcgatgaatt ctatgcggac acgacccggc tacaaactcg acatccggat ccccgcgcac
      121 ttggcgatgc cctgaaagcc gacattctcc acacgaccgg cttgacctgc acgatcgccc
      181 tggcttccgg aaaaaccgcc gccaagatcg ccgccgacgc acataaacct gacggcctgg
      241 cggtgattga accgggcgcg gaagccgcat ttctggcccc gttgccgatt cgctcgttgc
      301 ccggcatggg tcccaaatcc acggaggccg tcgagcgctt gggggtgcgg acgatcggcg
      361 atttgcttct ccctcgattc gaatcagccc tcgctcgcct cctgggcacc cgcctggcgt
      421 cgtttcaatc gctggcacgg ggcctcgatt ccgatccggt ggtcgctgat cgcgaagcca
      481 agagcacgag tcacgagacg accttcgacg aagatacgaa cgatccggct gtactggagc
      541 cgatcatcca tggatttctc gaaacactgg cgcatgagct tcgcctggag ggtctggctg
      601 ccggttcatt caccctgaag ctcaaggatt cgcattttcg catcaccacg cggcaacgga
      661 agtttgccac gccgttgaac tatgacccgg acatgtggcc ggagattcgc caggccttgg
      721 gagatttgct tcagccgcga ttgcggtacc gcctggtggg cctgggccta tcggatctgg
      781 taccggctcc ggaaccgctg ttcgaccgcc gtcaacgcga tgcggttgcc gccctggatc
      841 gactgatcga acgacatggg accggcgtcg tgcgtctggg cgccctcccg cagagtgaag
      901 ctgggacgag gaaacgaaga cctcgcacgc cagactgatt cccgagcgca ccacgttcgc
      961 catgcattcc tggtggcaga ctttttctcc atcctgataa gatgcacgcg aggattgatt
     1021 gaacgtgatg cctacaaaag tgaccacgcg agatgaactg gtttctctgc tggccgagcg
     1081 ccgccagcgg caacagcgcg tcgtctttac aaacgggtgt ttcgatctca tgcacatcgg
     1141 acatgtccgg tacctgcagg cagcccgcaa tctgggagat ctgctcgtgg tcggcgtgaa
     1201 ctccgacggt tcggtgcgcg cattgagcaa aggtcccgat cgccccattg tcccggacgt
     1261 ccaacgggcc gaggtcctcg ccgcactggc ctgtgtcgat tatgtggtca tcttttcgga
     1321 gccggatccc ggagccctga tcgcagcgct gcagccggat attctggtga aggggggaga
     1381 ctggccgctt gaccggatca tcggccgtga aaccgtcgag gcacgcggcg ggcgcgtgca
     1441 aaccattcct ctggtccccg gcgtctcaac cacgaccctg gtacaacgta ttcgctccac
     1501 cacggcataa ggatttcgtg ccacctgtcg tcccatcgca tctcacgaac tggttcgcgc
     1561 ctgcgcgaga ggccctccgc acgggatcgg gcaaaccctg cttgatggga ttgcacggct
     1621 ccacggcagg cttcgggctc gcgctcctca cgctcagcgc gccggcacaa cctctcgccg
     1681 accgctcgtg gctggttgtc gccaaaacgg atgacgaagc ggagcggctc tatcgcgaca
     1741 cgcttttttt cagaaccctc tgcggccaat caggcgatga cctggccctg ttcccgaaat
     1801 gggaaacgct gccctacgaa tcgacggtgc cccacatcga cctcgtctcc cgccgcatgc
     1861 aaacgctcaa tcgactctgc accacagctc gcacggtgct ctttacgtcc gtgccggctc
     1921 tgacgcaacg tgtgcttcca gccctggtct tcaccgaatc catactgcag tttcaaccga
     1981 acagcgatct ggagcgcgaa gtgctggtct cgagcctgtt gcgcctcggc tatcgcaagg
     2041 gatcagtggt agagatcccg ggcgaattca gtattcgtgg aggcatcgtc gacatttatt
     2101 cgacggccta tccggacccg ctgcgagtgg agtttttggg cgatacgatc gaatcgatcc
     2161 gcttttttga tccggccacg caaaaatcga ccgataagat caagcaggcc tgggtcctgc
     2221 ccgcgcgaga actgatccgc cccgacgatt caccagacgc gcttgcgccc ctggcggcag
     2281 atgcggaatg gcatgccccg tcggtctatg gggcgatgga cagcctgctg gattattttc
     2341 cccagccgcc cgtgctggta ctggatcagc ccggcgccct caaagctcac acggcagaat
     2401 gttggcagac cattgaggaa gggtacctgc gtcacgaaga tcgcacggat ccgaatccct
     2461 atccgactcc ggatcagctg tacttcacgt gggatcaact cgtcaccgcg acccaggggt
     2521 atgcgaccct cgccctggag ccggtcaccg cgccggatgc gagctgggaa ccggtactga
     2581 cctgtccggc gcaaacaccg gccagcgtgg gactcggcca gcgcggcacg gcgtttagtc
     2641 agacactgga agtcctcgat cgcctgcgtg aaggcgggcc cgtggtgctg gtcgcccgga
     2701 gtcagggcca agtcggccgg ctgctcgcgc tcttcggcga gcacgatcga ccggcgatgg
     2761 agtggaaacc cgcagccctc tcagcaggcg gcgcgcagaa ggccccattt tcggtcctga
     2821 acggcgaggt gtcttcggga ttcctttcgc ccgacttgcg gctcgtcgtg ctgaccgaag
     2881 aagaactgtt cgcaaagggt gcccggcaca aaccgcaaca caagagcaaa gcggccacgt
     2941 ttctctcttc gctcgaagat ctgaacatcg gcgatttcgt cgtccacatg cagtacggca
     3001 tcgcaaaata tcaggggctg cgccggctct cggtccagga cttcgacagc gactttctgg
     3061 tgctcgagtt cgccgggacc gataagctct atgttccgct cgaccggctg agccaggtgc
     3121 agcgttatgc gggggccgat gcgcatgtgc cgcgcctcga ccggctcggc gggacgagtt
     3181 gggcaaaaac gacggcgcga gtcaaaaaag atatcgaaga aatggcccat gagctcgtcg
     3241 atctctatgc caaccgcgag ctggtccatc ggacgtcgta cggcaaggac agcatgctgt
     3301 atcacgagtt tgaggcggcc ttcgaatatg aggaaacccc ggatcagcgg aaggccatcg
     3361 aagatattgc gaaagatctg gcctccacca aaccgatgga ccgcctcgtc tgcggggatg
     3421 tgggctatgg caaaacggaa gtggcgatgc gggccgcctt caaggccgtc gaagaaaacc
     3481 ggcaggtggc cgtgctggtc ccgaccacgc tgcttgccca tcaacattac gacaatttcg
     3541 ccgaacggtt cgcgcccttc cccacccgcg tggcgctgtt gtcccgcttt caatcgccca
     3601 aagacacgaa ggccatcatc aaggatacgg cggcgggtct ggtcgatgtg ctcatcggca
     3661 cccaccggct cttgcaaaag gatgtgcagt ttcgcaacct cggcctggtc atcatcgacg
     3721 aggaacaatg gttcggcgtc aaacataagg aacgcttaaa acagctgcgt acccaggtcg
     3781 acgtgttgac gctcaccgcc acgccaatcc cgcgcaccct gcaaatgacc atggccagcg
     3841 tgcgcgacct gtccatcatc gacacgcccc cgtccgggcg ccttgcgatt cgcacccaag
     3901 tgcttcggtt cagcgaaaaa gcgatccgcg aagcgattct ccgcgagctc ggccggggtg
     3961 gacaaaccta cttcgtccac aatcgcgtgg aaaccatgga acgaatgggc gcgtggctcc
     4021 aggaactggt ccctgaggct cgcattgtga tggcacacgg ccagatggat tccaaacccc
     4081 tggaagccgt gatgctgaaa tttttccacc gtgaagccga tatcctgatc gcgtcggcga
     4141 ttatccaatc cggcatcgac gtgccgaccg ccaacacgat catcgtcaat cgcgccgata
     4201 cgtttggcct ggcccagctg taccagctgc gcggccgggt gggacgtggt ggtgaacagg
     4261 cctacgccta tttcctggtg ccggatgaag gcaatctctc ggacgacgcg cagaagcggc
     4321 tgaccgccat ccagcaattt accgagttag ggtcgggatt ccggattgca gcggcggacc
     4381 tggagatccg cggagcgggg aatctgctcg gcaaacaaca atccgggcac attgccgccg
     4441 tcggactcga tctctacctg caaatggtgg aacaagccgt ccagcgcctg aggggccagg
     4501 tcgtcgagga ggagccggac cctacactgc gtctcagcgt ctcggcctac attccggaag
     4561 actacgtggt cgacagccat caacgcctct cgctctacaa acggttgtcc tcctgcggac
     4621 aactcggcga cctcgcgctc atgcatggcg aaattgaaga ccggtatggg cctccgccgg
     4681 acccagtcga acgactgttc gaactcatgc agatccggct cctggccaaa caactccggc
     4741 tcagttcggt ggtcgagcag acgcatgcgg tcgtgatcac cttcgatccc aaggcgaaag
     4801 tttcggaaac cgctgtgcag gccctgatgg accggtataa aaaacggctg cgatttctgt
     4861 cgcctttgtc gtttgaactc cagatgccgc atgacgactg gagtttggtc tttccagaac
     4921 tcaacgcaac cttgcaaacc cttcacgtct gtggtaccaa caatcaagag ccacggaccg
     4981 gctctacctg atcttcccag aaggatgctc cgccccgacc gcatgacatc accgcagaca
     5041 ccagacatat ccccctggcg ggcacgactg accggcaccg cgctagcctt gtgtgcgctg
     5101 ggctcattgg ccggctgtac cgaaccgccg caggaggaac cggtgatcgc gatgatcaac
     5161 ggccgctcca ttacccaagc tgagtttgac atccggtggg aagaactctc ccaggccacc
     5221 cgcgcccggt atgaaaaaga aggcgggaaa cgccgcttcc tcgatgaatt gatcatgcgc
     5281 gagctgttga tgcaggaagc gcgcaaacag ggcttggacc aatcggatga catccgggag
     5341 aaaaccctgc gctatcgcga gcagctgatt ctcgatgaac tcctgaaaga ccggatcaag
     5401 accaaggttg aagtgtccaa agaagaattg gatgcctacc tcgaaaagca tgccaaccag
     5461 ctcctggcga atcccaaagt tcaggtttcg gtcatgttgc tacccaacgt ctatgcggcc
     5521 aaggatctga agcgccaggt ggaaggcggc gggaacttca cccgctttgc cctgcgctat
     5581 tcgatcgacg aacgcagccg cgccaaaggc ggcgacctcg gcccctatcg aaagggcctg
     5641 ctggagcctg aactggacgc gttgattccg tcgctgcacc ccggcgtcct cagcgatccg
     5701 atcaagaccg acaagggtta ctatctgatg aaggtcagtc ccctggagcc ggaaatttta
     5761 caggccgacc aggccacccg tgaacggctt cgccaggaac tcctggctga gaagcggcgg
     5821 aaacgccttg atgatgtctt tgccgaactc cgcaccggtg cgacgattcg catggctgat
     5881 gcggcccgtt atgtgaccga tgaaccaggg cgcccctaac ctcctgcgca acggcttcct
     5941 gcccgcgttc cgtgttcacg cctccttcct tttcatcgca agatcgctcc ctttctgaag
     6001 tctcgattgt gtacaatccc cttaccgtga tgaatgcccg attgtctttc ctttctcatt
     6061 gtgtccgcgc cgtgagacca acctctctgc aatcgctcag ccgaccggct ttggccggcc
     6121 tatgccttta cgtctgggta gggctgttcc cggccggtcc ggtcgatgcc gcgaagttgg
     6181 aagaccgtat tgtcgcagtc gtgaataccg acctgatcat gctctccgag ctcaagcgcg
     6241 acttgcttcc cgatcaggag cgccttcgca agctgtacag cggcgaggat ctcgaacgcc
     6301 gcctcaagac cgcggaagcg atggccgtca cgaagatgat cgagcgcaag ctgcagctcc
     6361 aggccgcgaa gaacaagggc gtggaggtgt ccgaccagga agtcacgcag gccgtgcagg
     6421 aaatgaaaaa acagggcgaa gccctcaacc aggcagaccc caacaccacc agaagcgttc
     6481 gtgaacagct gacgttgatg cgtgtcgtcg accgggaagt tcgagggctg attatggtcg
     6541 ccgattcgga aatgaagcgg tactacgagg agcaccagga tcgtttcgcc taccccgagg
     6601 aataccagtt gagccaaatt ctcatcaaac cgcggacacc ggacggcctc tccgtggcgc
     6661 agggtcgggc cgaagccctc cttgcgacgc tcaaacaagg cgaacccttt gaagatctgg
     6721 cgctgcgttt ttccgatggc gcagacgctt cgcgcggcgg gcggttagga ctggtccggc
     6781 aaggggaact gattccggca ctggagcagg cgctcacctc ggtgccggtg gggcagatta
     6841 caggaatcgt agaaacggct gaggggctgc atatcgtgcg cgtcgatgac aaaaagccgc
     6901 gccagtttcg cccgttcgaa caagtgaagg cggaaatcca gtccctcgtg tttcagcaga
     6961 agaccgaaga ccaatatcag atctggatgg ccgatctgaa aaacaaggcc tacatcgaga
     7021 tcaagtttta acaggatgct gaaaaagtcc gccagcagcg ttctcgcctc gttcagaccc
     7081 tcaacgtacc tccaagggta cgcttcggcc cttcactcgc tgcggccttg ctggacggtc
     7141 tttttgagca tcctgcggga gtgttctttt gcgatgtgat acaagcgtgc tatcgaagtt
     7201 ctcttgtgcc agattcgtgt ttctgcaatc cattaaactg cgcgcgaatc tcgccccata
     7261 acgcgtcccg cccctcgtgg gtttcggccg aatacaaaag aaccggcact tccgccggca
     7321 gcccgcaagc gacacgcact tcggtgagag tcggtttccg ctcgctctgt cgcaacttat
     7381 ccgccttcgt cagcacgatg atgaggccac aacccagcga ctgcacccac tggatcgtca
     7441 tgacatcttg cggcatccag acgcgcgact cgatcagcaa aatcaccgcg ccgagcgatt
     7501 gccgctgttc cagatattgt tcgatcatcg ggccccattg cgcgcgcatc gttttggaga
     7561 ccttggcata gccatatccc ggcagatcga ccagtgacaa gagtggcagc ttcggatcgg
     7621 aagtacgaac ggtaaaaaaa ttgacggcgc gcgttttgcc cggcgtcttg ctgaccttcg
     7681 ccagtttctt gcgatgcaag agcgaattga tcagcgagga cttcccgaca ttcgatcgcc
     7741 ccacgaacgc aatttccggc aggtgatcgg caggaaactg ttctggggac acggaacttt
     7801 tgataaactc ggcgctgagt agtttcataa tgatcgcgtc aggcagcaat gcgggatggg
     7861 cgtcaacttg atccacagag gacaagtgag ccatctcgta ggctcataga tagagtgtag
     7921 ggaatgagca agtcaacagg cagtctcttc gggcggatac tgttttggat gaccgtgctc
     7981 atcggtttgc cggccgccgc cttggcgttg tattggctgg ccacattgcc cgacgtctcg
     8041 cggctggcga aacatcaccc cacagaaacg gcggtgatgg aagcccggcg ggcccaagcc
     8101 aaggaacaag gccatccgct gcacatcaaa ttcgcctggg ttcccctgtc ccggatttcg
     8161 cccgcgctcc aacgtgctgt cgtcgcggct gaagacgcct cctttttcgc gcacgaagga
     8221 tttgactggg aaggcatcaa ggacgcggcg ctctacaatc tggaggtcgg cgaattcaaa
     8281 cgcgggggca gtaccatcac gcagcagctg gcgaaaaatc tctacctgtc atccgaacgg
     8341 tctctgctgc ggaaagcccg ggaggccttg atcacccgct ccctggaaca ccatctgacg
     8401 aaaaagcaga ttctcgaact gtatttgaat gtcgccgaat ggggccaggg cgtctttgga
     8461 gcggaagcgg cggcgcgcca ccatttcgga aagtccgccg aggatctcac gatcgaggag
     8521 gcagccctgc tggccgcgat tcttccttcc cctcgtcgct atgacccgat tcggcacacc
     8581 gcctatctca ctcgacggca acgccacatc gttcgctggt tggagagagg gaacggccgg
     8641 aaagcggcct cgatcaaccg actccctccg gagagtctcg aagtcgttcc tgtggagtaa
     8701 taccactcac tcgtcacgcg caacgccagc actatgtgga ttcgccgacc gacgtgtagt
     8761 cgcgctctcc gaaaatcgcc gactcgtgga cgtaatactt aaattcgagc agatttcctg
     8821 acgggtcttc caggaaaaac gtgcgatgct cgattcgtgt cccgggaaac cggcggcgtg
     8881 gctgttgata aaacgtaaga tgctgggcct ccgcgcgatc ggccagcgcc tgccattctt
     8941 cctcccgcgt caacaccagc ccgaaatggc gaggatagat acccttctgc cgatcgagtt
     9001 cgtcttcaga gaggtgggcg acgagttgat gcccggcgag gccgaaggtg acggccgttg
     9061 gagattcccg ccccaggaca cacccgagcc cctcgacgta gaattgtttg gcagcggcca
     9121 aatcgtggac gggaaaggca agatgaaaaa gcgccggcat aggctgagac ctcgtgtcac
     9181 tgtatcagcc gatgactgcg ctaggcaaac cggtcgcgca caatcgcgcg tccgtccggg
     9241 ccgatgatcc cggcaatcat atgcggggtg ctgtggcgaa tcgtgcaccg gccgtccggc
     9301 gagagcacta agacgccggc tgcaccttga acgcgctcca ctacttttcg cagcacgcgg
     9361 ttggccgcgg ccaccgggct cagaccggtc gccaacccat ccgcaatttc cttggcgacg
     9421 gcaacccgaa tgatgctctc tcccaatccg gtcatggata cggcaccggc gtcattgtcg
     9481 gcatagaccc cgcagccaat cagtggcgta tcgccgaccc ggccaggcaa catcacatcg
     9541 acaccaccgg tcgacgcgcc ggctgccacg gttcccttca catccaacgc caccgcgcct
     9601 accgtttctt ttcctaagct ccttgcacga aggcggccgg ttttcacaat ggcctgatgc
     9661 agctgcaacg ttcgactcac ccccgctgct ggtctcaatt gcttgggctg ggctgtcagg
     9721 cttcgcctcg aacgcggagc gcgctcgagt ccaaaatagt ctgcaaagcg actggcggac
     9781 ttcccgacca gtaagacatg cgcggttttt tccatgacga gccgggcggc ggtgacggga
     9841 tgaaggatgc cctcgatgga agccaccgcg cctgcgcgaa ggtcccggcc ctccatgatc
     9901 gaagcatcca tccggcgtac gccgtcgagt tggcgattgg agccgcgccc cgcattaaac
     9961 agaccggagt cttccaacaa tcgaatggcc tcttcgacca cagacaacgc cgggacaccg
    10021 gcctgcaaca gacgatggcc ttcggccaac gtgcgttcca cgcaggcggc ttgagcaacg
    10081 gtcatggcgc ggcgtcccgc gccaccgtga acgaggagaa ccggtcgtag gcgtttcaat
    10141 tgaggagcag atctttcacg cgacggcagg caggatcttg catacggtcc aagtcagacc
    10201 ggacaaatcc cagtccggtc acacagagct caaagttttc tgacagcttg cggaacaacg
    10261 gagtatcctg ctcgggatcg taatgaccaa attccgcgac caggccatag gaccgcttcc
    10321 cggctttcac gtaatcgacc agaaaatcct tgtgatagat tctccccacg ctcttgaggc
    10381 ggcggagata ttccggaaag agcccggtca taaacagcgt aaagtcgccg atgtggcgat
    10441 gcacgtcacg ctcacgttcg aacgactgtg cctccaacaa gacttcggac tcaaataaca
    10501 tctcgacgac cgagtcgagc ggacggccct gggtattctg gattttgtag agttgatcgg
    10561 tgtgcgcgaa ttcgacgagc aggttggaga cgtactcggt gacgttcaga tcgggccacc
    10621 cgaggtgttc ggtgaaactt ttctccgtca atgctccgaa gagctgccgg agcggatggc
    10681 tgcgggacac gggacttccc atctcgacct cccgggcaat ccttgccctg tgaaaatagt
    10741 ataccagacc attctcaatc cgagcggact ttctctctga ctccttatcg gcatcccgga
    10801 tcaagacttg aggtccgcgt gtgctcccgg tcgccggcta tccacaataa acgtgactgg
    10861 cccgtcattg agtagttcca cctgcatgtg cgcgccaaag acgcccgttt ccaccttgag
    10921 gccttcgtgg cgtagtctct ggacagcctc gtcataccat gcacgcgccg cctccggcgg
    10981 agcagctagg tcaaaccccg gccggtgccc cttcgcggta tcaccgagca gggtaaattg
    11041 cgagacgacc aacagcgctc ctccgacatc acgcagggag cggttcattt ttccctgatc
    11101 gtcattaaaa atccgtaggg cttgtacctt ctccacaaga taggagagat cgccttctcc
    11161 atcacccttg gccacgccca ataacacgag cagtccgagg ccaatgcgac cgacgacctg
    11221 cccttctacc tcgactgacg cctgtgtgac acgctggatg accgccttca tctcgagttc
    11281 gccacaatcg ttcgcaccta tcgggcattg cgcaattgcg tcaatgtccc ttccaaaccc
    11341 aacagacgcc gcttcaacgg taatccgctc gaaaacccgc ccaaggagcc gtcatgcgca
    11401 atgatgcggt gacagggcac gacgataggt accggattcg ctcccagcgc catgcccacg
    11461 gctctggcat actgcttccc acccacacgc atggccaccc actgatagga tcgcacgcgg
    11521 ccgtaaggaa tccctgcaat cgccttccac acctttcgct gaaacgaggt gccggcagac
    11581 caatcgatag gcagcgcaaa ctcgcgacgc tccccgtcga gataagacaa gagctgggct
    11641 tgcgcctctt tcacaataga cgccgcggcc tcgtctctgt gtgaatcatg acgaacggtg
    11701 cgaacagacg gaaacaaatc aatggacgtc actccctttg cagaagccgt gacggtcatc
    11761 cacccccaag acgttttaaa ggtcctcgtc tccgtcatgt catgcgaccc cgaattttcg
    11821 cgcgacccgg ccgagtttac gcttgaccaa tcccatgacc acatccagct cgtccgaccc
    11881 tagcatgaga tgcatgccga gatacgcgat gacgctgaac ccgatccccc caaacagcat
    11941 gacggacttc acgatccatt gaccggtttc catccacagg ggcgcgctcg ccacccacag
    12001 acagatgccg gccattggaa tggaggccgc cagtacccgg ccggccgatc gcacgacagc
    12061 accccaatcc acccgcccca agcgccgatg caacacggct acgagaatac ttccgttcag
    12121 catcgaggcc aatgccgtgg ccagcgccaa gcctgcggcg tccagaatcg tcatcaacca
    12181 gagagacaag ccaatattcg ccccgacggc aatccccgct gttatggccg gagtccgcgt
    12241 atcctgcagg gaataaaagg cggacacgat aatacggact ccagcaaatg cccagagtcc
    12301 gacggcgtaa cagagcagcg cggtcgctgt cgccagggtg tcggtcttgg tgaaggatcc
    12361 gtgctcaaac accaaatgca cgatcgggta ccgcaggagg atcagcccgg ccatggccgg
    12421 gaggatgatg aaaaaaatca tcctgaggcc gaacccgatg gtcgtccgca attcatccag
    12481 tgctccgcgc gcagcctgcg ccgacagagt cgggagaata gccgtggcga gtgctacacc
    12541 aaaaataccg agcggaaact gaatcaaccg catcccgtaa aacaaatacg tgggcccgcc
    12601 ggagaaatag gacgcgagga tcgtgctcac cgtgatattg atttgggtca ctgacaagcc
    12661 cagcagtgac ggcaccatca gaagtccgat gcgtttcact ccgggatggc ccgggttaaa
    12721 ccgcaggccg aacaacatgc cgcgtctttt taacccagga agctgcatgg caaactgcgc
    12781 caccccgccg acgacaatgc cgatggccac cgcaaggatc ggctcggata accacggcga
    12841 taggaagagc atgcaggcga tcgtaaaaat attgaaaaag accggagaga aggccggcgc
    12901 tgcaaagtca cgtaacgaat tcagaatccc catcgccaga gccgccagac tgataaagat
    12961 caggtaggga aacatcagct gcgtcaacag cgtcgttaag gccaacttgt cgacgctctc
    13021 tcgaaacccc ggggccagca gccatacgat gccgggcgca gccagaatcc cgaccagcgt
    13081 cacggcggtc acgatggtga ggagcgtcgt gaaggtcgca ctagccaact cccaggcgtc
    13141 acgcttggct ttcaaggtgt gatattcggt aaagacggga atgaaggccg cggacatcga
    13201 tccttccgcg aacaattcgc gaagcagatt gggcacgcga taggcgacaa aaaacgcgtc
    13261 cgcggccggc gtggcgccga acagcctggc caacaccatg tcccggacaa atccgagaat
    13321 gcggctggaa aatgtggcga tgccgatcag acccgccgcc ttgacgacgg aatgggtttc
    13381 atcgacagct ggttgagatg gttcaggagt cgcggcggga tcggacattg gcggaattgt
    13441 agcggaatgg agtgaggccg tctattgtcc gacagaatcc agccgtcaca gatcttgtat
    13501 caccgcattc ggcaactcgt tgggattatc gccctcacgg gaaggacagg cccgagcgag
    13561 aagaactccg gcggcttcga ttccatgaca gagaccacca gcgaggtcac cgaccttcat
    13621 acgctccacc acgaccgaaa caatgtcctg ccactgatcc actgaaatgc gcccctgcag
    13681 tgcctggtcc ggcaggatat agacctgtcg ctctagcaat gagaccatca gcaacagccc
    13741 ggtccgttca cgggtttgtg caaggccctg ttgagcaaag gcccgttccg cgcgcatgtg
    13801 cactttgtga cgcatgcggt cccgggaagt gaagagccgc agaagcatgg gccaggttcc
    13861 aacccagacg ccaacggaat aggcgagcac cgtgatgccg actaaccaca cggcattcga
    13921 agcctgccat ccccaaggca accaggcggt ttcgatcgtc acaagtaccg tcaacgccag
    13981 cacggcactc aataggccgg cacgatgatg cgcttcgcga tagagccctg atcgccccac
    14041 gatcaggggt acgatctccg cgctcgtgtg ctgctcggcg gcctgtacgg ccgcctcgac
    14101 acgcgcccgc tcagcttcag tgagtgccgg cttactacca gtcgccactg gcccctcctc
    14161 ctccgaaatc accgccgccg cccgagaagc cgcccgatcc tccgaaagaa tcgcccagtc
    14221 cgcccgatcc ccaccccccg tgccgtgtgc tgaaccaggt gtcatcgaat ggaccgctgc
    14281 ggcttcgccg tcctcctccg ctcgcttgtc ccaatccact catcaaactg accagcgcca
    14341 cgcttgcggc gccggcgata agagccggga tgacccacgg tgcgaccaga aacgagagtc
    14401 cccctcctgc gacgggaccc agtacgcggt gagcccgaga gaagaagaga ccaatgacca
    14461 cgcctacaat gacggcaaca atcgtattgc cgatcacctc accatcggaa tgaacagcgg
    14521 tcggcgattc tggtgcccta taggttccct caatagtgtg caggacagcc tgtacaccgg
    14581 ccgtgacgcc ggccgggaca tcaccggcgc gaaatttggg agcgatttca ttgcggatga
    14641 tttgcgccga tcgcgcatcc gtcaagactc cttccagccc gtatccgact tccagacgaa
    14701 ttttacgttc tttgatcgca atgaggagca gaacgccatt gtcggtgcct ttggcgccta
    14761 acttccaggt cgtcgcgaca cggtgggaaa aatcgaacag cggttcgtcc tctaatgaag
    14821 gaacaatcag tactgcgacc tgattggagg tctttgattc atgcgcctgg agggtctcgg
    14881 acaattgagc cgtggtggcg gggggcagga catgcgcgag gtcgaccacg cggccgctga
    14941 gcggaggtac atcgagagca cgggccggaa ggctcggcac cagcgtgaac catagccatc
    15001 cggcaaggac agcccaccct gctcggccca tcagccgcat cgccattgct cctgtcacag
    15061 gaagccgacg ctagaacttc acttccggtg gcttggccac agccttttca tcggccacgg
    15121 tgaagttggc tttttcgtcc aaatggagaa gaaatttggc cgtcagattc gtgggaaagt
    15181 agcggaccat cttattaaac tccgccacct tgtcgatgta tcgcttgcgc gcgacggtga
    15241 tgcgattttc ggttccttcg agttgacttt gaaggtcacg gaatccttga tctgccttga
    15301 gattgggata attctcggca agtgcgagca ggcgccccag ggcactcgat aaactctgtt
    15361 gcgcttcttg aaacctcttg aaggcggctt cgtctttgag taactccggc gtgatctgca
    15421 cgctggtcgc cttggcgcgc gcctgcacca cgccttccaa cgtatccttt tcatgcgccg
    15481 catagccctt gaccgtgttg accaggttcg gaatgagatc ggcccgacgt tggtactggt
    15541 tgatcacttc gctccacgtg gctttcgtgt cctcatcgag cccttggaga tcgttgtagc
    15601 cgcatccgga caaggccagg agaccggcga gtaaaaaact tgcgcccatc aatcgtgcca
    15661 ttgagatcat cgctcctcct ccgcgccgaa cggtgacagc gcttcttcaa gatctaaggc
    15721 ttttgtcgac gaagtcaatc atccagtctg ccgcccaccg cttgagatgt cgcttcgctc
    15781 acaaccttgt tatacttcgc agcgggtaag gttattgggt cgcaacggaa tcttatggcg
    15841 ctcgatcgtt ctaccgatac tccgggaggc tttcaggttc ggcaccgtgc cttgggaatc
    15901 ttccaaggca gctcgatcgg gttggccttc tggcaccctt cgtcccatat gccggaatat
    15961 ggtctctgcc gctttgctac ccgggctaaa gcccaggact atgtcgattt cctgtcgtca
    16021 cctgcgtgcc cggaaccatt gagtcaggca gatctcatca tagaagattt cgatcacgcg
    16081 gaacacgagc gtcttacgac ggaataccca caggcctctg cctgggaaac tccactctag
    16141 cagaatggcc agcaggatgg atctcggggt ctcctgaagt ttcgtgggat tcgcgaggcg
    16201 atgctgcgta gcggagagtc gttaggcagg agaacgaggt ccgaccgagt ccgtcttgga
    16261 ctgtacgaac gccagtaggc gcttattttc ttgggctgac cgcagaaatt tgaaggcgat
    16321 accgcgagaa ctcaccgatc tgaccatggc agcgacttcg atgggagcct gttcattctc
    16381 cagcacgatt tgcaggtaaa agatatcgtc ctcatgcacc gatgcctgac tttcaatgat
    16441 gcaaccgccc attgaaatat cccgcaccgt cccctccccc cgcacctttc ccccggaaaa
    16501 cgacaaaaac aggcggacag gaatacgcag gtgctcgcga cgatcaatca ttcgagccgg
    16561 gtaccgatag ccgaagcggg acgccgagaa acgaaagctg catgactggc aatgaaacgg
    16621 tgacatgcag gcgagcgact gcaaccgctc catgagagtc gagcaggggg aacggaaaac
    16681 tttatcctgg ccgcactgcg ggcaagtcaa cggttgtgcc atatccaata atcggcgcct
    16741 ctcagggcct gtctccttca cacgcgcggg atagggctcg cgcgacgatc tatacatcct
    16801 gtgcggacgt catccagcac attccggtgc cgatgtggcc tgcaatacat ttttcggaaa
    16861 gctcgcgtct gtcgccagtt caatcgataa aggatggtcg gagttctgtc aagaaactca
    16921 acggttctgc gcgtagacca tgaggtaact gagtgggctc aatctgaagg agggaagaat
    16981 tcgacttctt gtcgcaagcg tcgctccatt tcgactctca tttcacgtca gattttatac
    17041 agaatgttcc tcgctgcttc tcaatttgtc taagcaggac aagtgaagct cctgatatcg
    17101 cggggaaatt ttgattaatt cattttaacg tcgcactaac acctctttaa tctcccactg
    17161 ttagtctctc cctcacatgg gcacggcctt cacgcgttgg aggcacatcc taaggaggtg
    17221 gacaccatga catgcggacg atgcaaagga ctaatggttg atgaatggcg acccgatttt
    17281 tcgccggaaa cgtttgtctg gcggtgtatt aattgcggat cgatcgtgga tccgttgatc
    17341 gaacagaatc gacgtactcg gatggccgag caattattgt tggaaccggt cgaggtattg
    17401 cagtagatcg aaccctcagg gcaggggcac tgggtgctcc tgccctgtag cacgcgaggt
    17461 agagtacggt ggcattacat atcgaagtcg gcccaaagca cggtgtgatc ggaagcatcg
    17521 gtggcacggc gaggctctaa atcaacatcg acgcgctgac atttctgggc caacggcgct
    17581 gtggccagaa tatggtcgat ccgccaccct ttattggcag ctaaggcact tggcgcacgg
    17641 tagtcaaaaa acgtgaactg ctggcgatct ggatacagtt tgcagaatac atcttcgaag
    17701 ccccacgcaa ttgtcttccc ataggctttg cgcgcgtctt cgtggtagca gacatgtttc
    17761 agatgtttct ccgggctgtg gacatcaatg ggtcttgggg ccacattcat gtctccacac
    17821 cagatcgccg gctccttcgg ggagagatgc atatcaaagt gcttcagtaa gcggtcgtac
    17881 caacccagtt tatattgata tttcggggaa tcgatctcaa acccctgcgg cacataggtg
    17941 ttgatgattg gaatcccctg aatgacgacc cgcagcagcc tggcatcctc cgcttctccg
    18001 ccatcgtcaa atccgtaaca gacaaattca ggcttcgtcc gactcagtac ggcgacgcca
    18061 ttgtaggcct tcataccccg gaacgtgatc tcatatcccg acgtcgcgag cgcggcaaga
    18121 ggaaattcgc tgtcttgcac tttggtttcc tgcaggcaga gcacgtcagg ctgatggcgc
    18181 tccagccact gcaagacgat cggaagacgt ttccgcagtg agttgacgtt gaaggtggcg
    18241 actttcatcg tgggcgcgaa ttctaccaga gcccaccacg cgcaacaacc gaggtctgca
    18301 aaatactacg gcctgtgatc gggaggttcc gatcacaggc cgcaggcgag aaaacgcgcc
    18361 gttttccgtc tacccgttaa tagcgggtcg cttccttcag gtcgttcttc atgccgccca
    18421 tctcttgaac ggtatcgtcc gcagcacttt tcatgtcggc cgctttcgcc cgggccgcat
    18481 ctttcttcgc tttcactttc gctttgacgt gttccttctt ggccttcgca tcggctttgg
    18541 cttgtttctt tttggctttt gcttcgtccc gcttcgcctt cagctcgctc tttgcagact
    18601 ctttcttcgc cttcagatcg ctcttcagtt cttctttatg ggcattcatc tcgtcggaaa
    18661 aggtgcccat atcctgcttc atcccctcgg acatttttcc catgcccaga tcgtcggcag
    18721 cggcgaatga gctcatgccc acaaatgcag cagcgaccat cacagcgatt gaccttctca
    18781 tcaacaaccc ccctcttggt tgaatatggt gtcaggcatc ccggcaatcc gacgggcctg
    18841 cagcatccat caataccccg aagagtttcg tttgccaatt tttttgccgt tttttacggc
    18901 aaggaggagg gtcgcttaca gtcgagagcg cggccatccg caagagagac ggatcacttg
    18961 aactacaagc ggccttactg acgaatagtc gtgaggtagc gccggagaat ggcctgctcc
    19021 acccgcgatc ggccgcggtc cggtgcagga aatcgcagga ccagatgaat ccgccccgcc
    19081 tcgggaagct ggatcgtgat gcgtggctca ggcgaagggg cttccaataa atttcgttgt
    19141 tccagcaact tcatttgccg acccatttga tccataaaag gggcgcactc tacctttgcc
    19201 gcgccaagta gggcttgttc ggcggcctgc caattgtcag tgcttaagag cggaaccgac
    19261 aagacataca aaccatactc ttgcgacgga ctctccttaa tgagcggatt gccgaaaagc
    19321 agactgttgg gaaagacgac ggttcgcccc gtgtacaagt gtgaggtctg accagggccg
    19381 atctccagaa gcttcgtggc aaatacatca tattcgagga cgacgccacg gtacgtaccg
    19441 agttgaatcc ggtctccgac tccatagacc ccgccgccga cgcgtaatgc cgcgccgctc
    19501 caacagagaa tcagctcctt tgttgccaga actaaggtag ccgccaatgc gacgagcgag
    19561 accgcgaagg cttccaactc gtgcgcccaa atgaccacga gtcccacaaa gaggcacagc
    19621 accatgctat tgcgggtggt cacgatccac cggcgccgcg cgtcgatggt tagcgcttgg
    19681 ttatttttaa tccatcgaac gagaaccgtt cgcgcaatga ggaggataag gaggagaagg
    19741 atggacttga gcagatcgaa gaagacactg gagccgatca ccggcagcat atcttgcatg
    19801 gttttttccg tcattgtcac tgcactacgg taccatcctc actcgtgcgt ggcacgcatt
    19861 cttctctttt ccaatctttc aggcgttttt gctcgtcaaa tgacaaggta tagcggtagc
    19921 aatacagcgg ctcccgatag ggttcatcgt taccagccag actttttcca aagggtgacg
    19981 gcgaaggcgg ggctttggtt tttttgccgg cggccaggct gctgggatcc cagggatgaa
    20041 cttcccggtc tcccatcggt acgcggtagg tccaaacggt atcgccgcct agtaccggcg
    20101 tcttggccgt gctcggtggg ccgaacttgt cttcgatatc ggcctgggtg agcttgtgca
    20161 cacccttatt caggtaggtg tcccgccagg gtccgccgca gcctgacaac atcgccaggc
    20221 acaacagcat gcctaacctg ctccatcgaa ccgacaacgg cagcaggcga ttgctcacgt
    20281 aacttaagcg gttatcgttc cgagtcacgt tgttcccgca cccagcgaat cgaacaatac
    20341 atcgcatacc cttgagccat catcccggtg accagcaagc ccaatgcggt ttgcagccac
    20401 atggtgttgg gaaactccaa cccgtagatg ccaagcatga agcctccggc aatgaagact
    20461 atgcccattc cgcgcgcaat caatgcattg gtccgcaaat cagacatcgt tcgcctcgtt
    20521 tctcaccaac ctgttacgct acaccggcac atcaggtcgg ttcaactctt cccttcatct
    20581 acgacttcgt tttcgagcga atcgcagcgg ctctcccctc gagcgcgtct gcttcaggag
    20641 tgcgaccgat ctttcgtaac gctcccgcat aatcgatcac actcttggcc acatccggat
    20701 ggtcaggccc taattgtttt tccctgatcg ccaacgccct cgcgaagagc gccgccgcct
    20761 gtgctgtatc gccttgagcg gcgtacatgg cggctaaatt gctcactgcc aaggccacct
    20821 cgagatgatc cggaccgaga agtttctctt tgatcgcgag tgcccgggtc aacagccgct
    20881 gtgcgtccgc gaattgtccg tgcttgcgat gcagtacccc gagattgttc aacatggccg
    20941 cgacatccag gtggttctcg ccgtggacct cctggtaaat cttgagcgct tccaaataga
    21001 cgggttcggc ctctacaaat ttcccttgcg tgctatacac ctgagccaga tgcgccaagg
    21061 tcacggcgac gcgccggtca tggggaccga actcctccgc tttcttcaac gctgcagcat
    21121 aaatctgttc ggcttctccc aattgtcctc gctgcacggc cacttcacca gcctgcatgg
    21181 ccgcctccca cgtctgttgc ccacaagacc aggccatgaa cagcaagagc gcgcacaaac
    21241 cgacgagagc aggccgcctc atatccgccc catctgctcg aggatggatt cagctggata
    21301 ggtcacaatc tctgccagcg tcggatgata gtgcgggatg cgcaacaagt cctgcgccgt
    21361 cccgtgaaaa tacatgaccg cgatgagttc gtgaatgagt tccgccgctt caggccccac
    21421 gatctgcgcg ccgatcagtt cgccattgtc cgggcgacac aacaatttca cgaatcctgc
    21481 gagagctccg agacacatcg ccttcccatg gtcggcaaac gggtacgaag ccgtcagata
    21541 gggaatcgcc tgttcacggc actggcgctc actgaggccg gcaatagcca cttgcggatc
    21601 cgtaaacacc atttcgctat ctaaccgatg gtcgatgacc cgcgctggca catccggatg
    21661 agtcgcgttg aaggcagccg tttctccttg ctggatcgcc aaatggacga tcggcgtgag
    21721 atcgttcaca tccccaacgg caaaaatatg cggttgcgag gtccgcatat cagccccgac
    21781 gacgaggcga ccatcgatga ccttcacgcc agccgcctct agattgagtc ccttcaggtt
    21841 aggacaacgg cccagagcat gaaagatgac ctcagccgac cggctgtgcg agacaccatt
    21901 ctgccggaaa tgcacggttt tcccggttgg ggatgaggtc acctggtcga aggcgacccc
    21961 tgtgagtact tcgatgcctt cgtcctcaaa agcagaggcc aacgcctgac cgacatcctg
    22021 gtccatatcg gacaacagcg tcgcaccacg ctggagcatg gtgaccttcg tgccgatccg
    22081 ggaaaaaaat tgcgcgaact cgaccgccac caggccgccg cccaataccg cgagcgagac
    22141 cggtgcatga cgcacatcga gcatttcgtc gcttgtcacg taccccgcct cagacaatcc
    22201 ggggatggcc accttcgcca cactggatcc ggtggcaatc acaaacgcgt cacctcgaat
    22261 gcgcaaccct ccggcacaaa tctcatgagg agagacgaat tcggcgtgcg attcgtacag
    22321 cgtgaaccgc ggatcgcgca acgcggcgat gcgataatcg gcaaactcct gcaccagccg
    22381 gttcttccgg tcgacgatgg ccgagaggtc agcccgggcg gtgacaggcg ccaacccgaa
    22441 ctcccgcgcg cgtttcatga gcgccatgat ttcggctgac cggagaatgg tcttcgtggg
    22501 catgcagcca cgcaaaatac acaagcctcc caacggcccc tgatcgacga tcgccacatc
    22561 ggctccggct tcgcacgccg tccgcgcagc ggcataaccc gccgacccgc cgccgatgac
    22621 cacgacatcg tgccgacgct cgttcttctg agagagttgt gatggcatgc ctgattcgat
    22681 agactcgctc gcaggttcga aaccgcaagg atacaccatg atcaaacccg gtcgctatcg
    22741 tcactataaa gggaacgact atgaagtgct cggggtggcc cggcattcag aaaccgagga
    22801 ggaatacgtc gtctatcgtc aactctatgg aacaggcggc ttgtggatca gaccgatgac
    22861 catgttttcg gaatcggtct ccatcggcgg cgagatcgtg cccagattcc ggcggttaga
    22921 ggaaggtcca gcgtgagaac gtcgccgcct gcccccggcg ataacccgcc cgatcgacca
    22981 cattccacac gagggcctgc tcgatcagga accgccgccc gctgctcgat attctgactc
    23041 cccggtaatt gtcgatgtat ccctgggtgc gggcgcgttc aagcatcgat tcacgctccg
    23101 tacggttcac cggttccgcc gtcagacggg atggcatatg caccagctgc tcccaggtca
    23161 tctcccagag cgtgagcgcg agttgcgatc cgtaattcag gatcggatcg tcttgcatcc
    23221 catgtgagac caccacgatc ggggcggtga acagcgcctg agcctgagct tcagccccat
    23281 ccacccgctc catcaactct cgaccgacac aatggcgata gctgtcgagc agccactgag
    23341 accactcgac aatttccagt cgccgccata cttgatttga atcgtccacg gctcctcggc
    23401 gggagggatc gggcgcaagc tggccctcct ccgcatgacg tgggtagtag cacgacggct
    23461 taccgtctgc caaggtgtgc taagcggggg tttttcgcag atgtcgcacg aacaaagctt
    23521 gtccgtggct accgaggagc gtcatgacca agtcgccgcg gcggagcgca gtgccatcat
    23581 tcttcgcccg tgagtcatgc ccgacgaccg agcaagacca cacttccgtt ggcgcatcct
    23641 gccccttcgt tttgaggacc acacggcctc cttggccgac ctgataggtc agatccagcg
    23701 ggacgccttc gagtatccga tccgggctgt cgagcatcgg ggtacgcgag tgagctgact
    23761 tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcgagta tccgatccgg
    23881 gctgtcgagc atcggggtac gcgagtgagc tgacttggct ccctgctcgc gtgaatactg
    23941 gaagacttga tagggaaaca gatagggggc ctgcggatct ttgaagcgat aggcagaatc
    24001 gaatgccacc aggctccagg taccgagcag atcctcaccg gaacaggatc gcagcgacga
    24061 ggccggaacg gtcagcccgg atcgcgtcag tgactttgca tgggcgcgat cactcgccag
    24121 ggccgatccc gacgccagca tgaccgagca gcacgccacc agaagcgccc actgccatcg
    24181 cgccatgtgc atctccatct cagacgggat taaggatctt tgcgtgaatc tgccgcggga
    24241 gagggagcag gtaaggcctt gggagctcgc ggcaggacgc gatactcgat cagtctgcac
    24301 gtgcagaacg agacctgtcg ccattcagcg gtaatcttca cgatcgccga accgcagatg
    24361 gagcacccca gggcccgggg atcgcgcttg atcgtccagc gagggatgct gatgtggtcc
    24421 gcgttgatag gttcctgttc catggaaacc ttatcggtgc gcggttttga aatctgaaga
    24481 ggcatttttt tccggagccc ctttgccctt gatgcgatgg gcctggtcgc atatataata
    24541 gctccgcgat gagcaccgcg ccctcaatct ctcgcaaggg cgcgggttta taaggagcat
    24601 ggccatggct gcacagacac tgttcgaaaa aatttggaac gaccacgtgg ttcgggcgga
    24661 accggatgga accacgttgc tctatatcga ccgccatttg gtgcacgaag ttacctcgcc
    24721 gcaggccttc gaagggctga cggtcgcggg tcgcactcca cgccgatccg ccgccgcact
    24781 ggcggttccc gaccacaatg tcccgacgac cgaccgccgc gtcgccattg ccgatccggt
    24841 gagcgccaaa caaattcaaa cgctggatga taactgcaag acgtttggca tcaccctctt
    24901 cggcatgaac gacatccggc aaggcgtggt gcacgtcatc gggccggagc aaggattcac
    24961 cctgccgggc atgacgatcg tgtgcggcga ttcccacacc tcgactcacg gcgcgttcgg
    25021 tgcactcgcc ttcgggatcg gcaccagcga agtcgagcat gtgttggcaa cgcaatgtct
    25081 tgtgcagaag cgtccgaaaa cgatggaagt tcgtgtcgac ggccaactct cgccgcgttg
    25141 ttccgcgaaa gacattatcc tcgccatcat cggcaagatc ggaactgccg gcggaacagg
    25201 ttatgtcatc gaatatacgg gttcggccat tcgcgcgctc agcatggaag ggcgtatgac
    25261 gctctgcaac atgtcgatcg agggtggagc acgcgcgggc atggtcgccc cggacgagac
    25321 cacattcaac tacatcaaag gccgcccgat ggcgccgaag ggcgccctgt gggaccaggc
    25381 cgtcacagcc tggcggaacc tccacaccga tgccggcgcg aagtacgatg ccgtcgtgga
    25441 attacgcgcc gagacgattg cgccgcaggt gacgtgggga accagcccgg gcatggtgac
    25501 cggcgtggat ggcactgtgc cggacccccg cagcatgagc gatgacaaac tccgtcaggc
    25561 tacggaacgc gccatcgaat atatggctct gaaaccaggc atgccgatcc gggatatcaa
    25621 gatcgacaag gtcttcatcg gatcctgcac gaattcccga atcgaagact tacgcctggc
    25681 cgcctccttc gccaagggca agaaggtcgc cggcagcgtg cacgccatgg tcgtccccgg
    25741 ctccggcctc gtgaagcaac aggcggaaca ggagggctta gaccgcattt tcaaggaatc
    25801 tggcttcgaa tggcgcgagg cgggctgcag catgtgtctg gccatgaatg ccgatgtgtt
    25861 acagccgggc gaacgctgcg cgtcgaccag caaccggaac ttcgaaggcc gtcagggcgc
    25921 cgggggacgc acccatctgg tgtcgccggc catggcggtg gcagcggcga ttgaaggaca
    25981 tttcgtcgat attcgtcagt ggtcataagc aggaagacaa ttcgttcctg acgccgggtc
    26041 cgctgatcac agcggccggc agacacgacg cacaaaagga cataccatgg acgcattcac
    26101 cacactcacc ggcctggtcg cgccactgga ccggctcaat gtcgataccg accaaattat
    26161 tccgaagcag ttcctgaaaa ccatcaagcg caccggtctt cgggaagggt tgttttatga
    26221 ttggcggcga cgaaaagacg gatcccagga tcctgaattt ttcctgaacc agccgcgcta
    26281 tcagcaggcc tcgatcctcc tgacgaggga caacttcggc tgcggatcct ctcgcgaaca
    26341 tgccccctgg gcgctgctcg accagggatt ccgatgtatt ctcgccccca gcttcgccga
    26401 cattttctac aacaattgct tccagaacgg cattctgccg gtcgtcctga aggctgcaga
    26461 aattcaagcg ctgttcgagg gcgtggctgc acatgatggc tacacgttga cggtcgactt
    26521 ggcggctcag caggtcaaca cacctaaggg catggcctat cacttcgaca tcgatccatt
    26581 ccgaaaggac tgtctgtatc gcggattgga cgcaatcggc ctgacgctcc agcacgcagc
    26641 gcaaatcggc gaatatgagc aacgccgccg cactcaagca ccctggctgt ttcaagacgt
    26701 gaggtaaccg tcagaaaagg agatctatga cgtggtggga aacccttctg tttcctgtag
    26761 cctggctctg gagacccaaa cttctcttca cgttgttgtt tttcggcggt gcagcctatt
    26821 tgctgatcgt ctttaattgg agctactcgg acggcgaccg tgccgggtac ctgcaaaaac
    26881 tgtctcgaaa aggatggttt tgcaagacgc aggagggtga gctggcgatg acgaccgtgc
    26941 caggcgtcgc cccggtgctg tggagcttca cggtgtggga cgagaacgtg gccaagaaac
    27001 tcgacgggca gatggggaag cgggtcgtcc tgcactacaa agagtttcgc tatatcccca
    27061 cgacctgttt cggtgaaacc ccctacttcg tggatcgtgt cgaggttctg gactgatctc
    27121 ctgattcaga gccacttctt ttgcttgaag aagtacagca tcgccagtcc ggtcaacgcc
    27181 atgacgccca gtaccgccgg atatccccag ggcgatttca actccggcat gtgctcgaaa
    27241 ttcatcccat agatgctggc gatgaaactg agcggcatga aaatcgtggt gatgatggtc
    27301 agcaccttca tcacgccgtt caagcgataa ctcacgctgg acagatacac ttccatcatc
    27361 gacgagacca tttcgcgcag ggtttcgatc gtgtcgccaa tttgaatgat gtggtcatac
    27421 acatcacgga aaaatacttt ggtggacccc tggagaaatt gtccatccga ccgcgacagg
    27481 ttgttcatca cgtcccggag aggccagacg gagcggcgga aaaagagcag ctgccgcttg
    27541 agtgcatgaa tgtcccgcag tgtctcagga ccgggattcg tcacacagag atcctgtaac
    27601 gcttcgatct tttctcccag tgtttccaga atgacgaagt atcggtcgac gatcgaatcc
    27661 atcaaggcat agagcaggta atccgcgccg gcatggcgca aacggccttt cccattgcgt
    27721 agccgttcct tcacgccttg aaagacgtcc ccgccgttct cctgaaacga gagcacaaaa
    27781 ttctctccga acaccaggct gacctgctca acattgatgt cgccctcgct ttggccttcg
    27841 tagagcatct tcagcaccag atagccgtac gtgccgtaat cgtcgagctt cggtcgttgg
    27901 tcggtgttgg cgatatcctc cagcaatagc gggtggaggc cgaacatttt cccgaaggct
    27961 tccaacacct ccatcttatg gatgccgccg acatccaccc atcgaacggt cggctccccc
    28021 gtcgccacca cctcgtccgg cttggacacc gtgcgttcct ggtactggcc ttcgccgtat
    28081 tcgtagacgg taatggtgac cgtctccgac ctcttttccc cgatatggac cagtgtgccg
    28141 ggcgggaggc ctgccttacg tgaatgtttg tgtttcaact tcatcgtact gccagcttgt
    28201 acgcggaacc tgcaagaggg tcaagagccg cagcatccgc atgaagcgac gtcgggcttt
    28261 gttcccgtcc caagacctgg taggttgaag agaagaagac tcgcccattc ccgggagcca
    28321 tgactatgga gtacgaactg accatcctgt cgcaagccat gagtgaagcc ggccgtgaag
    28381 cgcttcgctt agccagcgca ggatttgaaa cgtttacgaa agccgatcag tcaccggtga
    28441 cctctgctga cctggctgtg aatcgcattc tccaggacag attgtcgaca gcctttcccg
    28501 aggacggctg gctttccgaa gagacaacgg acaacgacac acgcttgagt agaaagcgtg
    28561 tctggatcgt cgatccgatc gatgggacgc gttcgttcgt gcggggcctg ccggaattct
    28621 gcctgtcggt cgcgctggtc gaaaacggag tgccgaccgt ggcagccatt tttaaccccg
    28681 cgaccggcga gtttttttca gccattcgag ggcgtggcct ccgtgtcgaa cgactgtcgg
    28741 attctgatca accgggcttc acctcatccg aacggccggt cgcgctcgtc aatccatggg
    28801 aactccgggt cggccggttc gaaggcctgc aggaccatct ccactgccgc ccaatcggat
    28861 ccatcgccta cgcgcttgcg ctcgtggcag ccggtcaagc ggatgcggcg atcaccctgg
    28921 ccggcggaaa tgaatgggac gtggctgcgg gagttctgtt ggtagaagaa agcggagggc
    28981 gcgccacgac cgccgctggt catcccgtca ccttcaatcg cccagacccg cgcttaccgg
    29041 gaacgctggc cgtcgggtcg gacctcccgg cgccggtgcg cacccgcctg attcaacatg
    29101 gcctcgcggc cgcatcccat ccgctttgag ccttttcaac gcgcgcgcgt ctctgtacga
    29161 tggccgcatt ggctacatcc gagaggtatt ccatgaacgt cgccttgtta ggcacaggat
    29221 tgttgggcca gggcgtagcg gaacggctgc atgccaccgg ccacgacctg acggtctata
    29281 accgttccat caaaaagact caggcactcc ggcagcaagg catccgtatc gctctgactg
    29341 ccgcagaggc catgtcggca gcggacgtcg tgctcctcct gctgtcggat gccgcagcca
    29401 tccgtgcggt gttgttcgat cccgcggcca gtcgcgcgat tgacgggcgc acgatcattc
    29461 aaatgggcac gatcggaccg gccgaaagtt gctcactcga gaaagacgtc acaggtctcg
    29521 gcggccggta tcttgaagcg ccggtgctcg gcagcattgc ggaagccaaa gccggcacgc
    29581 tgctggtcat ggttggtgcg accccggagc actatgacaa gtgggctccg cttctgcgtt
    29641 atctcgggga agaggtgcgc ctgatcgggc cggtcggaaa agcggccgta ctgaagctgg
    29701 ccctcaatca gctcatcgcc gctgaaatgg cggcgttcgc attcagtctg ggcctcattc
    29761 gccgaggcgg cgtcgatgtg aatgatttta tgggagtggt gcgaaagagc gcgctgtacg
    29821 cgccgatgtt cgagaagaaa ctatcccggt tggagaaacg gaactatgct caaccgaact
    29881 tttccacgcg gcatctcctc aaagatgtgg agctggccct gtccgctgcg gaaaaggccc
    29941 gcctttcgcc ggccggcctt cagggagtcc ggtcactcct catcgacacg attgccgcag
    30001 gccttggaga cgtggactat tcggcgatct atgaacagat cgatcccctc ggctccgagc
    30061 acgggtagga atcagcgcgc ggaaggccaa ggctgattgt ccacaatcga ttgtaccttc
    30121 atcacatgaa atcgcccgcc ggccttggtg ggagctttgt caccttcaat gcgcgtgtac
    30181 caccaccgcc cctcgccttc ggaatagtcg ggggacaggg taatctcaaa gcccccttcc
    30241 cgatcatttt ctcgttgagc ccaggctcca gcccatcggt ggtcggaaaa cagatccgtc
    30301 cggaatcgtc cgtctttaaa atcgtaggac ccgtttccga atcgatctaa tctcaacggc
    30361 accaccatcc cgccttcttc atattcccat tcacccgcga gaactgcgtg ctccccgcga
    30421 ggcaaggaaa tttccccggc gggcggttcg gtccacgtgg tttcttgagg ccgtggcgca
    30481 tgactacagg cctggatgct taacaggctc agacccatca tcaggaaaaa ccattgattc
    30541 tgtttcattc tattcatccc gggtgtttcc ttccccctgt atgcatcagg gtaatactgt
    30601 acataccatg cgtatcccat gtgctcaagg ctgcgatcaa attttgctca tttccaccac
    30661 tctggattga ttacttccga ccaagcaccg atcccaccac acggcggaat gtcccttcta
    30721 agtaattgag aaatctctca tcaacccaat atctcaaggt cacgcggtcc caaccgataa
    30781 agaatcggca ggaaagatct gcgccgatat tccgacacgc caggattttg cacgaggcag
    30841 gagttcctct cgcaacccgg cgaaggctgc cacatccaat tgggagagac ctgttgatga
    30901 tgccatcttc agacaataaa cacgcagggg gcgaggctca gaatccctct cactcgtctc
    30961 ctctgaacat cctgctcgtg gaggatatgg aagataatcg cgtcctggtt tccctcttac
    31021 tgaaggcgtt tccgtatcgc ctggatctgg ccgaacacgg tgcggcaggc gtcgagaagt
    31081 ttcaatccgg ccggtacgac ctcgtcctca tggatattca gatgccggtc atggatggct
    31141 acgccgcgat tcgcctcatg aggcaatggg aaaaagagca gcagcggagc gagacgccca
    31201 ttctcgccct gaccggcaac acccatgacg aagacatcgg gaaggcccag gccgccggat
    31261 ttaccgccca tgtgatgaaa ccgctgaaga aggtcacgct gctggaagcc attcaacgct
    31321 atgcggctcc cttctcacgc aaggagtctg ccgcatgagc gacatggcac ctgtcacgtc
    31381 cagctgcggt gccggaccga tcgtggtcct ggtcgactct gcgtttgaac cgctgattcc
    31441 gaaattcatg gccaaccgga acaaagaact cgcgacgatg acggaggccc ttgcgacaag
    31501 cgactttgcg accatcaggc aactggctca tggcatgaag ggtgtcagcg gcagttatgg
    31561 gtttcacgcc atgacgacca tcgccggtcg aatggaggag gcggccaagg tcgccaacgg
    31621 aacatccatt cgccaggact tgctcgaact ggcctcgtac ctcaccagag ttgagatcac
    31681 gtacgaatga cgaggcccca tgtgctcacg tatctggtcg gtggcaggct caatgactct
    31741 acgctgccca tgcagcacgg tggccgggcc ggggagtgag aaaatcaaga agtatgcatg
    31801 tgtttgtaag aatccgtgct acaaaagaat gaactcgtcc ggatcaggac aatctgaata
    31861 ttcaggcccc tcactcatcg atacctaagg agcgatgtat ggataacccc gaaacaccca
    31921 aactcagggt tctcatggtt gaagatgagg aagacacggc gagcctcttg aagtttctgc
    31981 tggagcgggc caactatcag gtcgttcatg ccaaagacgg ccggcaggcg caggagctcg
    32041 tcaataccat cgcgcctccg gacatcgtgc tgttggacgt gatgctgcct ttcttaagcg
    32101 gattgcaggt actcaccgtc atcagaaagc gcgagggctg gaaaaaagtc ccgatcgtca
    32161 tgttgacggc cgacgggagc gagcatgata tcaagcgcgc cttggagagc ggtgcaaatg
    32221 attatatgat caagccgttc aacccgcgcg aactgacgag tcggctgaag cgctttgtcg
    32281 cacatgcggc ctgagcgagc gccgagagga atggcgtgct gggcctcgac cagtcagacc
    32341 tgacgctccg catcggcacc atcgcggcca tttccatcta ttcctccgta ttgcttctcc
    32401 tccttggcat tctcctgctt cgatacatac gcaacaacaa agaccggcgt cgacgcgccg
    32461 tcacggaaat ctggcggccg atactggcag aatgtgtggt cgaagtgcct gagacgcttc
    32521 ctccactcac gacccgcgac cacctcgtcc tgctctatct ctggaatcat tgctacgagt
    32581 ctattcgtgg agacgcccgg gaaaatctga aagaacttgc ccggcgaacg ggcactgatc
    32641 ggttcgcgaa ggaactcctc catgcccggc tgctccggcg ccggttgctc gcgatcgtga
    32701 cgctcggcca ccttcaagaa cgatccatct ggaacgagct cgccgccgcg ctccaggccg
    32761 ataacgcgtt cgtctccctc gtcgcagcca aagcgctgct gcatatcgat gcgaaagccg
    32821 ccgtccccct gatcattccg gtgatcagcc agcgaaagga ttggtctccg ctcaaagtcg
    32881 tggcgatctt cgaaccggca ggtgcggaca tcgccgctga agccatagcg aaggctgcgt
    32941 gccttgccag cccggaaatc gggtcgcggt tgattcgcca cctggcagcc actcaaagcc
    33001 agcatgcctt gccgccgtta cgggacatgc tgcagaaagg gacgccgtcc gatgatctac
    33061 tggcagcctg cctgttcctg ttcggagaat gcagtgatcc tcgagatatc gtgacgatcc
    33121 ggacgcacct ctcgcatcca acctggtatg tgcgactcca agcggcgtcg gcattgggga
    33181 aggtggggat tgaggaagat gaagcacgac tcatcgccct gttggatgat gagcattggt
    33241 gggtgcgcta ccgggccgct gaagcgttgt cgaatctccc ctggatgacc acggagcgtc
    33301 tgacccgcgt gcacaacaca ctcaccaccg tcgagtcaca ggaacacttg gttcctttta
    33361 tggctcaatc gaatgcgaag gctcagcatc cggctcgcat cgcctagcgc atgaccgcgc
    33421 acaccgctcc ttcactacag gcccatgttc actgtgttcg aacgcgatga cgacgtgcga
    33481 gaagccgacg gggtcaaaga actgggcgtg aagaggacgg tgtaggtgcc ctgcaattga
    33541 gggaatgtga ccatcgttcc atgttgcgcc actccccgca gcgggttgcc tgagcgtgcc
    33601 acctcccatc cgggcggcac ctcgagcgtc aagcccgcca aagagactgg aacgttcaac
    33661 gtcacccgca tggcatcggc atcgcgcacg acatcgacct ccacccgatt acgcgccgcc
    33721 caccatcgtc cgaattctcc caccgagccg aaccacgcgt aggttcgcac gccctccacg
    33781 aaccctttct caaaggcgag tttgtgatcc agcacattcg gatgaatgag cacgacgaca
    33841 gagcctccgt accggctgat acgtttcgcg acctccaggg cctgcggaag acggtctccc
    33901 atcttcggta acgcttcatc ttcgatcgtc acgggaaact caaaaatctc gacctcgctg
    33961 tcggtcagac gatcgtaatt cagttggtat ggcaaatggg tcagcgagtt attcgccgtc
    34021 gccgtggaac tgtaccgata gccggtcgcc aggagcgctt gcggaagtcc aaagggattg
    34081 gacaattcgc ctggcctgaa agaaacgatg gattggccgg aaaagtgatc gatgagaaac
    34141 ttgctcactc gcaattcgcc caggatgctc acattgtagg ccgtcgtccg atccttcacg
    34201 aacggggcat acgatggata ggattcgcgg ccgcttccca tgggaaactg attgaacacg
    34261 cgtgaatggg cgatcgtatg gctacccaac tccatgccga gatccgctaa ctgcttcaca
    34321 taccggacgc cctcgtcgtt aaagaagata tcgtcgttat aatcgcgaat gtatttcacc
    34381 tgaatgaaat acgtgccgac gacgccctgg cgtttctcat actcagcata ctcaaccgcg
    34441 tgtttgatgg actgtgtaaa atccacatcg tgcgtcatca tgaccgacag cgacttgccg
    34501 aaaggcacgg ttcccagggt cacggcctct tcctgcgccg tgcgatacat cgctttgatg
    34561 agccgcaacc agacatcgag cgtcgggtcg aactcattgt cgaatgtctt gaccagctcc
    34621 tccccgcgat tgttgtatcc cttcagcagg aggaagccga gatccactcc aatcgcatag
    34681 gctcgaccgg gcccatgagc cacctgcgta acggcggcgg tcccatcatc gaaacgcgca
    34741 agcggagccg acgcgtcggt gtaaccatag gttcccatca cctcgatacc cttgtcacgg
    34801 atgccgaccc gcagccgttg ctcacgcggg tcggtcagat ccgccaacag aggatcggcc
    34861 gtagccaacg tcatgtcata gcgctgacgc gaggcaacag cgtcacgaaa cccgaagacg
    34921 tgattcaatc cgccacccag cacttgcacc ccgatcagtg tgccgcctgt cttcggaaat
    34981 tccgccagtg cttgcagttc ctccggtttc aacaccttcc cggatatcat gggatagaca
    35041 agcaccacct tatgcgccaa ggcctcatga acattgcgcg tcatacgaaa gggaatcccg
    35101 atggatttca acccgtgggc caacccgagc cacgatgagt ccggatcggt taagagaatg
    35161 gccaaccggc ttgtcgaacc cggttcgtag gtcgtccacg cggtcggcac gtggcgcggc
    35221 acgaccgtcg cgtcgtcggg gcccgccacc ccctggaaac gatgaaagtc tttctccgga
    35281 acctccatgt gctggacggg aggagcactg acgagaatcc ggacgccaat gacgagagac
    35341 acaaagagca gagcaatacc tgacacacgg acgagcgtgg accgttgcat agacctacca
    35401 atgatagatg aggttgaagt tcccgccccg tcggacatag aggaaccccc ggtcttcgta
    35461 gtatccggac gtttcggcgg agaatcgttc attaatcggg aacgtgacgc cgccttgaat
    35521 cgtgcgactt ccgacgcgcg taaagtcttg tgccgctacg atgcgttcgc ctgacgtgcc
    35581 gaacgagccg gaggcgaaga attgcaaccg atcggccggc cgccaggtga gctgagaggc
    35641 cagtgtgacg gcccccgtat tcggaataaa gtaaaccttc tccgtcagcc acagattgaa
    35701 gggaagaaaa atcgtcaggc ccggcatcaa tagatcgatg tcatcgtgct tgtaattcaa
    35761 acgccggtaa ccaaacgacg cttcaaacgg tgccagtgag gagtgcagcc cgcccaggcc
    35821 ttgcgcgact tcgccgacaa aggaatagtt gggcgagaaa tgcggattga tcgtggcctg
    35881 ccccgcgatg tacccccagg cccgcgacca caagggacta tagagctcgg ctgacattgg
    35941 cgtgtcgtga aatccaaagc gattgatcgg ttccactcgc atcaccagcg tttgattacc
    36001 gagcggtttg gcgagttcaa acaagaggtc gcgttcatcc ggctgattct tcgtataggt
    36061 atagtggccg tagccgatct tggcgtagtc ccggtaggga agacgcaggc cggccagccc
    36121 ctgtccgacg gccgcgcgcg gcgacacgag caattctgat ttcaccgcct tgatctgctg
    36181 ctcaatcgcc gggtcatgcg tgtcgtccag cagggcctca taccgctgca acgcttcgga
    36241 tcgatcgcca cgccaatggg ctacctcggc cagtccgcgc cgagcctcga tccgagtggg
    36301 ctcggcctgc gagactgccc cgtacaatcg ttccgcctca tcaaattgtt tctgccatga
    36361 caagacttgc gccagcgcga ggcgaatgtc ctgatcctca gggtgccgag ccagcacttc
    36421 acgatagagg gtcgtcgctt ccgcgtgagc gccctgccat gaaagcaccc gcgccagcgc
    36481 cgtgcggatg tcgtcattgt cagggtgccg gcgcaggaca tcgcgataga cgcgttccgc
    36541 ttcaggatat tgtttggcca cttcgaattt cgtggcttct tcaacggcgg ccccgataac
    36601 tcgctcgtcc gtgccctggg agtcgtgagt tgacgcaggg tgctcggcaa cctgtggcga
    36661 ggtcgtgcgc cattccgagc gaatagtctt cagctgggct tcgagctcgg gatcgtgtgt
    36721 ttcggcaacc aacgcttcat aacgttgaac cgcttctgcg taatgtccct gccaatgagc
    36781 cacatcggcg aggccgcgcc tcgcctcgac atgcgtcggg tctgattgga gcacgtcctg
    36841 gtatacgcga ccggcttcct cgaattgctt ctgccacgag aggacttgtc ccagtgccac
    36901 acgaatatcc tgatcctggg gatggcgcgc caggatctct cgatataacg tcgtggcttc
    36961 cgggtgaacc ccttgccagg acaggacccg tgccaacgcg gcgcgcacct cgtcattcgt
    37021 cggcgacagt tgcagttcct cgcgatagac ggtcaccgca tcggcgtatt gacgcatcgt
    37081 ttccagacgt cggccccgct ccaatgtttg agcggcgtga gcggtcaggg aatcgttccc
    37141 atcagcagcg ccggacggat tgacagggac gaccggtgaa cgagaagcgg ccttcaagga
    37201 ttgaaggcgc gcggccacgt ccgcatcgcc ggtcaatgcg accactcgtt catagtacgg
    37261 aatggcctga tcctgatgac cgctccacaa gtacacattg cccaggccgg tgagggcctc
    37321 gacatggtcc ggttcgtcgt ggagcacccg ctcgtactcc tctcgggcga gatcatagtg
    37381 tttctgccac gagagaactt gggcgagacc gacccggatg tcgtgatcga gggggtgcct
    37441 ggtcaggagg tcgcgatagg cggccgcagc cgcgtcccat tggccctgcc aggacagaag
    37501 tttggcgacc gacgcacgga catcgtcatc gtcaggtttg gcctggaggt attgccggta
    37561 cgccgcaatg gcttcctcgt accgcttctc gtcctccaag actctggctt gttgcagagt
    37621 ctggcgggga gaaagctccg gcgccatggc ggcaatcatc gtcgcgggtg caaagaacca
    37681 cgccgcggca agcgcaatca ccgcgagcag ccctgctaca gacgttcgtt gattgatcca
    37741 ggtgaaggag tcgttcatcc gtgaagactc tgtgcttcca acaggcacgc cctattcggg
    37801 gagggcccgg aatccatcga gatcgtgaat aatcacccca gcaaaactcg gatgcatcgt
    37861 ggtgtcgagc atatgactaa tagcctggga cacttccacg cgggaacgtc cggcaaaggt
    37921 cagacgttcg ggcctgaccg taaatcgacg atggatgcgg aaccattcgc cggatcccgt
    37981 cagccggtcg gcctcggaga tcggcttcca ccgtagccgt ttgtgctcgc gatctaacaa
    38041 ggcatcggca tggtccggac ggagatcacg ccgcagcacg acatgctctt caagcggcaa
    38101 caccgttgtc tcaacggcca gccataccgg cttctcactc acatcgccat accgcaggat
    38161 gtcctcggcg atgtcctgca cttcctcgag atcggtacga taactcatga ccgccacttc
    38221 atcagcccga tccatcacgg cataggcgag cggtcgacca cccacggtcg gggtagccag
    38281 ccagaaggga atcaccatcg acaatttggc gcggccgtgg atggcctcgc tcaccgtctc
    38341 gatcgcgccg agataccgac ggagctgccc gtcatcatcg aggaatccag gcaagagata
    38401 cggctcgata tcgagctgta tgccggatac cgcaccaccc cgcaccaggt gaaacagtct
    38461 ctgtacctta tcggccaaaa ggtgaggttc ctgaatggct tcggggtacc catccagcgc
    38521 cagtgcatca atcccggcct cctgcacctg agccatcaac gacgcgtagc cttcccagat
    38581 cgcctcatca tcgttgagtg atggcatttg gatgagcatt ctcgaacagg cttgcctgcg
    38641 gcaggtagcc agcagtggct gtggctcctg tatcgccgca cggtagttcc acacccagaa
    38701 accagtccca ccgggtggct tcttcaccgt attacgttgc gacaccgtca gctgctcgaa
    38761 ttcgatccgg ccgcgtggtc cttcagcgga cacgacgagt gccgtcacat atcgaaggtc
    38821 cactccgcga ccaatctctt tcaggggaat agtgagatca aatgtccccg tcactgttgc
    38881 cagcgactgg ttgtgctccc gctgatgacc ggccagatct tctatggcta ccaccacctg
    38941 gccggtggct cgcccacgaa gccgcagcat ctggtaggac atgagatcca tcggccttcc
    39001 ggcgggacgc agctcgatac gctgaccaca acgtcccgcg tccccggcag tccactccaa
    39061 ttcgagatgc gagtcgcttc gtaccgcctg tccatggcag ggaccaaaga catgggccac
    39121 cccgaatccc ggtaacagtt caagaatggg taaaaacggt cgataaggaa gaggacccgc
    39181 aggaggcact ggcccgctga atgccacggc tggatcggtg ctgttcccgg cataggtcag
    39241 ttcgatctca tggatcgtgg aaggaatcgg cggggacagg ggaatgtcct ccgcctgcgc
    39301 aagagaaagc cacaggatgg agatgacggc agcactgcat aacacggtcg cgaagaggcg
    39361 ccgcccgacg gcctctgtgc cccgaccgac ggatttcccg tcaatctggt ctcgagcgac
    39421 accatccctc gcggagacgc attgatcgat gtgggtagac acgctcattt caacttctga
    39481 gtggctgttc ctgctctact ttctcggcct gaccgccgga tacttaatgc tggatctcat
    39541 cgcgatcgtt cacctgcggc aatacctgca ggagcgtgtg ctggaaagcc tgccgcacag
    39601 ttacaccggt ttcgaaccgc agatcagcat cctggtcccc gcctataacg aggccgccac
    39661 catcgccggc tctattcggt cgctgctgca actcagctat tcggaatatg aaatcgtggt
    39721 gatcaacgat ggatcaaagg atgccacgat cgatgtcctc cgcaaagaat ttgatctgtt
    39781 tccgttcccc gaagcctgta atacgcaact gcctacggcg gaaatcagag gaatttatca
    39841 ctcctcgagt cataccaatc ttcgcgtcat cgacaaagaa aacggcggca aggcggactc
    39901 cctgaacgcc ggcatcaacc tgtcgatcta tcccctgttc tgctgcatcg atgcagattc
    39961 cattctccag ccggacagcc tgcggcgagc ggtgcaaccg tttctcgacg accatcgcac
    40021 ggtcgccagc ggagggacgg tgcgcatcgc gaacggttgc cgcgttgaaa acggcttcct
    40081 gaccaagatt ggattgccct ccaatctgct ggcactgttc caaatcgtgg aatacctccg
    40141 tgcctttctc ttcggacgac tgggctggtc gccgctgaat gccatgctga ttatctccgg
    40201 cgcattcggc attttccgga aagaggccgt cgtcaccgta ggaggctatc gatcggatac
    40261 gatcggggaa gacatggaac tggtcgtgcg cctgcatcgc catctgcgga tgggcgacaa
    40321 accgtatcgc atcaccttcg tgcccgattc cgtctgttgg accgaggcgc cggaggactt
    40381 gcgtacgctc aagaatcagc gcatccgctg gcaacgcggg ctgtgcgaaa gcctgacaaa
    40441 aaacctcgat ctcctgtgcc atccgaaggg cggcacgatc ggctggctgg cgtttccgtt
    40501 catggccgtg ttcgagtgga tcggcccggc cattgaggtg ttcggctact gcttcctgat
    40561 cttcagtttt gtcatgggat tcgtctcgat ccaggcgtgg ctgatcctgc tcgtggcggc
    40621 catcggcctg ggcgtgttgt tgtcggtgag tgcgctttta ctggaagaaa tgtcgttcca
    40681 cgtctacccg gatcagcgcg acgtgttgaa actgttgctg gcagccatcc tggagaattt
    40741 tggattccgc cagttgaatt ccctgtggaa gttaatcggc ttgtatcgat gggtctgcgg
    40801 gaagaaagca acctggggaa ccatgacgag gactgcgtcc tggcaggcgc ggtaaggatg
    40861 ctggtgctgt cactgagttt atgaggtcaa tcgttcgatc gcctggctgg ctcgctcgag
    40921 gtcggcgtcg gtcttcgcca tacaaaaccg cataaagcga ctgccctccg tcccttcaaa
    40981 aaatgcttcg cccggcacgc ccgctacacc ggtcttgtcc aacaaataca tcgcccgctc
    41041 ccgactggtg tttcccggga ggcgggatac gtcggccagc acgtagtagg ctccttgcgg
    41101 cacggctgga ggcaagcctg ctttcgctaa agccccgcag aaccgatcgc gcttccgttg
    41161 gtggtccagg gccagttcct tgtaatacga aggaccgagt gcgcggatgc cggcggccac
    41221 accgtactgt aagggcgtgg gagcgcagac atacaacaca tcactcatcg cgccgatggc
    41281 cttcgcccag gaagcctccg ccacgctgta gccgatgcgc catccggtga tgctgaaggt
    41341 tttggaatag ccgccgatgg taatggtccg atcggccatg ccgggcaatg aggccatgct
    41401 cacgtgttcc cgcccgtcga agacaaaata ctcatagatt tcgtccgtga tcactgacag
    41461 gtcacggcgg caggcgatct cagcgatctg ctccaattca cgccgcgtga agaccttgcc
    41521 ggatggattc cccggcgtat tgaccacgat ggctttggtc ttggacgtca cggcctgttc
    41581 caggctcttc acgtccatcg tccaatccgg cggccgcatc gtcacatacc ggggcacgcc
    41641 ctccacggcg agaatcgcct ggacgtggta agcataaaac ggttcaaaca gaatcacttc
    41701 atcgccaggg ttgaggagcg ccatgcaggt ggcttggaac gatccggtcg cgccggcact
    41761 caccgtcaca ttggtctccg gatcggcggt gattcgattg aacgacgcca ccttgtctgc
    41821 gattgcctgc cgcagttccg caatgccatc aaaccgagca taaatattgt gtccctgcga
    41881 catcgcctgc tgcgcgcctt gcacgaccac cggagggaca ggagtgtcgc acaccccttg
    41941 agacatattg atgccgttga cctttgcgca ggctagggtc atcgctcgga tatcggattg
    42001 agccaatcgg gctgtacgat cactcttctt catcacaccc tcaatgtcca ggtcatgtct
    42061 tgattcatga agtttgcgga tcctgcctcg caaggcagat gtccctacgc cgttcccaca
    42121 aaaaatgcaa ccggcaaaca gccatcgtgg gttctggaaa gagccaaccg ccttgcgaat
    42181 cagcgcgagg ccgttattca ggagcctgct gcagagacca aagcgtacga cgttcttccc
    42241 ggcgcgtcgt cacacgcgtg ggaagccggc tggttccggc acgaaaacgg tcatagtggc
    42301 cgagtacccg attgacatat agctgcgttg caggcagcgc cggtacaccg atggcctgcc
    42361 tgacgcgcgc ctcgccgaga tgataggctg ccaacgccaa cgacaggtct ccaccgaacc
    42421 ggtccagcaa tccgcgaagc agcgcagcac ccgcacggat gttgtctttc ggatcgaatg
    42481 gatccaacac gtggaacgct gccgccgtca acggcatcag ctgcatgagc ccaatcgccc
    42541 ctttcgagga cacggcgtcc gaattaaaat tcgactcgac cttgatgacc gcctgcagga
    42601 gcgcagggtc gagggcatat tgctcggcat aggtcgcaat gagcgaggca atgggttcgg
    42661 cggtaggccg gcccgccacc tccttcatga tcgggtcgaa ggccagccgg ctggccggag
    42721 gcatcagggc catcaggcat cccgccaccg ctaaaggaaa gagtgcacgg gccactcggc
    42781 tccagcgggt attccattca ggctgttgca tcgttgggag aagcggtctc gaccggacag
    42841 ccctagcggc cttcctcatc aaggctccga atgaggacgg cacggccatg ccgatcgaac
    42901 caggattgaa tgcgatcgcc cacgtgggcc ggccgatcga ttctggtgtt ttcgtcatgg
    42961 ggaatgcgat gttcctgtcc gcggacatcc cgaatcaggt aggcgccgcc ctcgacggcc
    43021 tgcacttggc cgacgactga ggcggtggat tgctcgcccg aggaaacggc agaccggctc
    43081 tgcgcgccca aggttccgga tacgatcccc atcccgaggt cttcctgagg ggaatgtccc
    43141 tggagcgcag actcggcgca gccggtcgca gtaaccatca agggcaggac aagcatcaga
    43201 gagcgacaat ccgcgagcaa acgcatcatg gccggaacct ccatcgatga atgacgtggt
    43261 gaacggtgca ggacgggtgt gagcgcaccg tcacgtcact attccatgga gcggcggaat
    43321 gctcaaggaa atgagggaaa ctgacgcatg aagcggagga gcgaaggaag aaagattagc
    43381 cggcaatggc ctgcgatcgg ttccggagag ctcgtgtcat agcggcagcc ctggcccgca
    43441 ggctctactc accgacacat cgaacatatt ccttaagcca acagcgatcc atcacgacct
    43501 gtaatcccgc ctggcgtgcg cgcagagcgg ccgcctcatc gacgactccc tcctggagcc
    43561 agacagcctt ggcctgaata cgtatcgctt catcgaccac ggcgccagcc tcctcggatc
    43621 gccggaagac atttacgaga tcaatcgaac cgggaacatc cgatagtgag ggataggccc
    43681 gttcacctaa tacatcagtc tcgcgcggat tcaccgggat caccaccttg ccctgctgct
    43741 gcatgtagct ggccacacga taggacgggc gggccggatt cgatgataat ccgaccacag
    43801 caatagtacg gcaatctcgc aagagacgtg cgatctcctc ccgggaggaa gcgtgatccg
    43861 tcacggtgca ccccagactt cttcgagccg ctgcgcacga ccgcaattaa atttatagaa
    43921 cttataccgg atcggattct tcttataaaa atcctggtgg tactcttcgg ccggataaaa
    43981 ctgcgaggcc ttggtcagtt cggtgacgat cggctgcgtc agccgttggg actgttcaat
    44041 cgcgcgcttt gactcctccg ccagtcgctt ctgctcttcc ccttggtaga agatgacgga
    44101 ccggtactga ctgccatggt cacaaaactg cgcgttcggc gtgaccgggt ccacattgtg
    44161 ccaaaaggca tcgagcaacg tggtgtaact cgccttggtc gggtcataca ccacttcaac
    44221 ggactcggca tgccctgttc cccctgcaga cacctgctcg taactgggat gctcgactcg
    44281 tccgcccata tatccgctga ccacagccgt gacgccggga accttctcga acacttcctc
    44341 catgcaccag aaacagcctc cggcgaaata ggctttggcg gcgccagacg gatcagcggc
    44401 ctgactctgc gagaccagca atcctgccag caccatcggc aacaccccaa gggcaccgaa
    44461 ccgacctatg atggacctca ttggacgcct cctctcgaac ctgagcgact ttcgatcatc
    44521 ataccagagc atagggactg ccgcgatccg gacagcgccc ccttcttcaa ctctttacgt
    44581 gcgtcgcaat cgattggatg ggtgtgtatg ataggcagca tgannnnnnn nnnnnnnnnn
    44641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    44701 nnnnnnnnnn nnnnnnnnnn nnntggacca gatccggctg ctgctgctca aactgtttca
    44761 tcatcagggt gatcgcttct tccggactat ccaccgaggc gatcaggcgt ccgcccagct
    44821 ttttgaagaa tttctcttcg gtcggcgtgg ccccgaccag aatgaccggc ttccccgcct
    44881 ttaacgccaa ggcaacttcc gacaccgtcc ctgcgcccag cagcccgcac acgacgacca
    44941 cgttgctgga catgacgttg acgttattgc gcgcattgcc catctccgta ataatcgcca
    45001 gatcgacata cttcgacaca cgctcgcgcg ctgacggcaa cacaccgatc gtcaaactac
    45061 cggggacttt cttggcgccc tgacaggccg cgtccatgac accgacatcg cggccacccg
    45121 tcaacacgac ccattcccgg cgcgcgatca gttcacccag gacccgggca ttatcaacat
    45181 cctttttcct ggccttggcc ggccccatta ctccgacaat gtatcgcatg cctatccctg
    45241 cgttctcccg ccgcattgcg gctgccacca gccagtcacg gccactgtcg cataataaat
    45301 ggcccaggaa tccggatcgt cggcgagccg gcaatactgc tcgatgtccg actccgttac
    45361 caggccggtg gccacatatt tctcccgcaa ttgttcggcg gagagtttca tcagctgcgc
    45421 ggtcgctcca ccgcccgctg acagatgtcc ttcatgctcc accgttaggt gactcagatc
    45481 ctgtcgttga aacaaggccg gcaacttcgc ccccagcgca tagtccatcc cgagcgacgt
    45541 gaacatacgc tcgattgcag cggtgacgcg cgtgaaagag gttctggcgt catccgacgc
    45601 ggtgacggcg cgagccgcct caaaatctgg ctcttcgatg acgacccacc cgcccggttt
    45661 gacacagcgc aacatccgct caaacgcctt ctgatagtcg gcgatatgaa tcatgacgta
    45721 ccgcgcatgc accagatcaa acatggcgag cgggagatcc gtgtcgcaga tatctccttg
    45781 gaggatgtcg atggtcgagg tgctgctgcc acgcaaaaag cgcggattgg tatcgagcgc
    45841 gaccactttg cccgaaggcc cgacacgttg ttccaaccaa cgcacaatgg aaccggcacc
    45901 cgcaccaact tccagacagt gccagccggt cgtcaggccg gtcgccaaca tacggcgttg
    45961 cgtcccggca tcgaagatac gctccaacat ctgcagacgc tgcaattcac gagcttcctc
    46021 ggtggcctga aacacataat ccgtcttcaa catgtcttat cctttattcg gcaccatgac
    46081 gccagctcgt ggccgcgcgt cagctgatcc tagaagcgag acccggtcag tgggacgtcg
    46141 gcctgggccc aggccgtatg caaccgcgca ctctcctgcg cagtctcttt ccgctgagcc
    46201 tgcagactat gcactaatcc atgcaaagcc cagccgttct cgggattgag gcgcaaatcc
    46261 tcgcgataca cagattccgc atcgccagga cgtcccgcca agagtagcac cgcccccagg
    46321 ttgtgacgaa ccggctgaaa ccagaatggc ggttcgctat acggcaccgt ggcttccaac
    46381 tggagggcct cgcgcaaggc ctggatcccg gcgtcgaaat cccccttccg tgccgcgatt
    46441 tctcccgcca gcacccgctc ggcaatcttt agcagcgtgc ggctgatctt gccttcagcc
    46501 gtcttcgccc tggccaatcc cttcaatgac tttcgaagat tcgagagctc accctccgcg
    46561 ctgccgaacc gcgtcgtcgc cgcaaaggcc agaccgcgca cgtaatgcca gagaccggtc
    46621 gtataccgca gttcttttgg gggcggcggt tgcctgatca actcacccca ctgaccaaac
    46681 cgggccatcg taagcaggac cgtgggcgca taccattcca gcgaaggctc tttcacgatt
    46741 tcgttgaccg aaatcttgct caggagatcc cgtgccgcct gcagagcttc cgcgcttcgc
    46801 ccttccatca ctaatgacgc attcaagaag tgaaggttgt gcgcatagta tcccgatgcg
    46861 tagttgccct caagggggtg ctgaagcaaa tactcatgat ccacggtcgc agccttggca
    46921 ttccgttctg aggcgtcccg atagcgcccc acccgcatgt aaatgtgcgc cggcatgtgc
    46981 accagatgcc cggcgcccgg catgagcgac ggcagacgca tggcgcaagg cagcccgcgt
    47041 tgagggtcgg gagacgcttc caccgcatga atgtaatagt gacaagcacc ggggtgatcc
    47101 ggctgatgct gcagcacggc ctccaaggtc gtcacaattt cctcggtccc gggctgcggg
    47161 cggccatcgg ctcgccaata gtcccagggg cggagcacca tcaaggcctc agcaaatatg
    47221 gtcgctgcat ccgcgtcctc cggatactca tgggccaggc gtcgcatcgc cttggcatag
    47281 gcttcgtcct tgcccttgcg agtcgcagct ttcttgaccg aataccgcgc ggcaagggcc
    47341 tccacatacg cccgctctgt gggagtcgca tgcgccagcc gtgccgtggc tttctgtacg
    47401 gcctctacgg ctcgcgattc tgccttggaa tccatccccg cattgatatt cggccccagg
    47461 ctcagcgcca cgccccaata gggcatgggc gcatcaggat cgagacggga cgcctcggtg
    47521 aaggccgcta gagcctcctc atggttgaag gcatagacca gcctcaatcc ctggtcgaaa
    47581 tacttctggg caaggtccga gtgggtagtg atcttatgct gcagcacacc gagattctcg
    47641 aacagcggca cctctgaccg ggcaattcca tcggacacag gcagcgccag acctgcgatc
    47701 accaagccgt agaccacccc tctcacatac cgcgcgtttt tcatgttccc cgctcctgct
    47761 cgtttttcgt atgcgagacg ggcaatgccg tcacactgcg acgactccgc cgcgcacaga
    47821 agggcacaaa ttagcagtcc cgacgagaag ttgctagtcg gagcaggctg agaagaaaga
    47881 agggctgaat acgtattgat gcgggacgat caaaatcatg gaccagcgag gccgcagcga
    47941 gtgaaggcgc ggaggcgtac cgtctggagg acgttcaggg tccgaacgat gtgagaacaa
    48001 agctgacggt tgttttcagc gtcctgctcg accccagaaa tctagcagga tgcgcaaaag
    48061 gatcgtccag caaggccgca gcgagcgaag aggcgaatcg tactcccgcg gtacggtgag
    48121 tctcggagtg acgcgagaac gccgctggcg gactttttcc gcatcctgct aggcgttgag
    48181 gtagggaatc tgggaagctt cgttggacgt gtcccgtcga tcacgcgatg atttcttgtc
    48241 ccgttcgcga tagggaatcg atcggggaga caccagggac agctccagct ctgcgggcgg
    48301 ggccatctcc atgtccttgc ccatgtacat caagacctgc tgctgggact tcaccagggc
    48361 acgcaagtca tgaagctgcg ccgacagatc ttccatctgc acctgttgcg tggcgacctg
    48421 ctcgcgtaaa tcatggagtt ccgcctgagt ggattgagta tgctggatga cccagtcctt
    48481 gagctcacgg gcttgggcgg ttaaggcacg ctggacaaac cccgcacgat cacgcaaccg
    48541 gaaaatgagg tcaccaacgg caccatccca ccggcgcgcc ggcaccagcc catgttccgc
    48601 ttgaacctga tgctcgatgt gccgcgcgac aggagcgccc agccagttcg acacccgctg
    48661 ccccaacggc tgttccgtcc gctcttcgga gttgccgtac tggcaccagt cttcgaaccg
    48721 ctccgtcata tcttccagct tgacgaacac cttgccgatt gccgcacgca atggggaagg
    48781 cgcaaacgga ttcaccgccc ccttctcacc cttatccggg gcgagcgtga gaagatgaat
    48841 gggtttttcc gccaaacgct gctgtttccg cgtcatagga cgatcgaagc cttccgagcc
    48901 ttatcggcaa acttttccca attcattact ctttccgata gtccgagtca atggcgagtg
    48961 ggtgtttttt tctcccccgg tccgactgac gcgcccggac cgagacaggg tctgggcgac
    49021 aggccagcct aaaacttggt cacgctcgag aagtgaaaat cgtggatgcg catggcaggc
    49081 accagcgctt ttcctgtttc ggacgtcacg gcctcagccg gcgcggtata ggtctctact
    49141 tgattaaagg cgcgcagcgg gctttcgtga aagcgaaagt ttcggacggc agacgtgatc
    49201 tccccgtttt ctatcaggaa tgtgccatcc ctggtcatgc cggtcagcgt caggtcggac
    49261 ggattgacgg gacggatgta ccagaagttc gtcacgagga tgcctcgttg cgtcgtgcga
    49321 atgaggtcgt ccaccctggt gccgatagga cgttcgcctg ataagtgggg cgactccaat
    49381 gtcgtgaggg gttcgatctg atgctcgcga gccgtatacc gatcatgcag aagttgggtc
    49441 aggacacccg actcgatcca gccggactcg atcaccggca acccttcgtg cgtaaagctg
    49501 tttcccagga ggtccgcatg ggccggttga ttcaccagcg agagccggcg atcgatgatt
    49561 cgtgagccca actttccgct gaatgggctg gtccccttat aaaaagactt ggcatccaac
    49621 atccagatca tccagctgag gagtccggcc acggcagccg gttccaggat gaccgtgtac
    49681 cggcccggcg gcagctcctg tggctcgtcc gccccgcgct tggccttgat aatggctgcc
    49741 agggtgcgct cctgcacgtg caggcggtcg atggaacgat gggccgcagc cgaccatccg
    49801 gtggcatcgc cggcctgcac ggtcaggctg aaccgccctt cggtgcgttc ttcataggcg
    49861 cgcaatcccg tctcagcagc caatcccaca gtcgtcgtgc tggacgacac ggtgccggcg
    49921 gcgttgagat tttccatttt gcattgcccg atcgcttcgc gcgcatagtc gagccgtcgg
    49981 gccgggccgg cggcagtcgt ttccggacgg gacgtgggca gggacaaata gctttgcgcg
    50041 ccgaccggcg gaaggtattc cggatcgtcg ggggagagcc gcgcgagcga ttcggcctgc
    50101 ttcagggctt cgcgaactgc cccggcggta aaatccgtgg tgctggcggt gccatgctgg
    50161 cgcccgaagg ccacggtgac ggagaggtgg ccccgccgaa gattgacgtt ctgaatgatc
    50221 tggttgttgg cgaaccgcgt ggtaccgccg tggctatcgc gcaatgagaa gaaggtgtga
    50281 tcgcccgtag agtgactcat cacgaggtcc gccagaaact caaactcctg gcgactcgtc
    50341 agtttcggcc atgaggacgt gatcgatgac gtcatgcgtc cgccttcgtg tgtccgctga
    50401 tcacatccac gcgccggaag cgggcgtggg atgcagcgtg agtcatccag cccgattggc
    50461 cgggctgccc tttaccgcac gtgatgaagc cataccggcg acgatgagtg gcatcggcga
    50521 cgccgtcgca gcggccccag aattccgggg tgatgccgtg atagatgaca tcccgcacca
    50581 tgtgcgtccg ccggccattc tcgatcagcc agaacgcgtc gccaccgaac tgaaaattat
    50641 accgccgctg gtcgatacta taggacccat ggccttcgat ataaatgccc cgcttcacgt
    50701 cggccagcag gtcatccaac gtggaggttc ccgcttcaag accgatgttc gcaatccgga
    50761 ccatgggaac cgatccccag ctatcggcgc ggttcgatcc gcgtgaccgc tcttccttga
    50821 ttcgtggtgc cacctctcga tttgtgcagt acccgacgaa aatgccctct cggacaatgt
    50881 cccacttctg gcagctgacg ccgtcatcgt catacccggt cgccgccagc gtccccggtt
    50941 cggtgttgtc cgccacgagg ttcacgtccg ctgagccata ccggtacgtg ccgcgtttgt
    51001 cgggtgtcag aaaactggtt ccggcataat tggcttcata cccgagggcg cgatccaatt
    51061 cgctcggatg tccgcaggac tcatgcatcg tcaaggacag gtgctcggga tccagcacca
    51121 ggtcgtaccg tcccgcatcg attgccggag cccgcacttt ctccacggcc tgttccgcga
    51181 tgcgcgcccc ttcacgcacc cagtcggcgt cgcgcacaag ctcatagccc gtacgcagat
    51241 ggggagtgtt gaacgaccgg ctcgcaaacc gtccgttatg gacggccgtt gccgtacaat
    51301 cgccctgcat cgccagcagg tcaaactcga gatgcgaacc ttcagtggag acgaacagtt
    51361 tgcgatcgcg ttgagcccat aggctggcgt gactcctcac cacaccggga tgccgctgga
    51421 tgatgtccat cgtactcaga agcagtgcgg tttgttcttc caatggaacc gcgaagggat
    51481 caaggcggga gggagtaaca atgcggtctc gatgcaccgg ctcgggggcc aggtggactt
    51541 tagtgatcgc gagagaggcc gaacctttgg caatttcgac ggccagatcg gcgacacgag
    51601 gaatttcttc gagtgaaatg atggagctgg cggcaaaacc ccaggcgccg cggtgcagca
    51661 cgcggatacc gaaccctcga tcggtggact cgcggatatg ggcgatgcgg cggtcttcgc
    51721 cgctgatggt ccgcgtcgtc gtatcaagca ggcggatatc cgcatactcg actccggccg
    51781 aagacacacg agacaaggcc aattcggcca gctcgtccca ctgcgatcca ctcatagcgg
    51841 cgtctttctc tgcgccgaat gcacaggcag tttcgcgtag tataacaaga tcgcggtcgc
    51901 tcgtgcctgc cttttcatcg acaggcggtt cacctacaat caagatggca tgatcactcc
    51961 accgcagaca ccccggcagc gaatcatgac gctgttgacc ggtacgttgt tatcttctca
    52021 tcagttggcg caacttgtcg gcatgccgga gcgccaggtg gaagatcacc tgacccacat
    52081 cgtcaagacg ctcgcacgcg acacttcacg aacatttctg ttggaaccgt cgcactgtca
    52141 ggattgtacg tacaccttcc gcgatcgaac caggctcacg cgaccaagcc ggtgtccccg
    52201 ctgccgcagc gaagccatta cctctccacg atatggcatc cgggaagagg ccggttcatg
    52261 atgatgcggc ctgtctcctc cagaccactg gaaaccctgc gagcgtggca gaccggcaat
    52321 gagcacgcca cgcgaccgat acatgaaagg accgtcgcat gaatacattc ctgacgtggg
    52381 tcattctcat cctgctcgtg agcacatcgg cctgcgggcc ggatcaggcg tcgcacatgc
    52441 tcgagaccgc ccagtttgaa gagcgacaaa ccaacaaggc ccacgcgaag gaactgtacg
    52501 aagacatcat ccgtcactat ccggacagtc ccgccgccaa gaccgcacgg acacggctga
    52561 cggaactcgc caaaccataa caacagccag ctgcgctgca gtgatggtcg cgaacgccgc
    52621 tggcggacgt ttttggcttc ttgttaaccc ggattattca tgaatgccgt cgcgaggtgt
    52681 tcgagaccga aacccgccga ggctgctcgc acgtgaggcc gacgcaggcg tactggcagt
    52741 ccgtcgagga ggccgagcga gaacagcctc gccgggcgac aggatcggac gccggagcag
    52801 gcattcatga atagtccggg ttacagccgg cgggggaacc agggaaagaa cgcattcgtg
    52861 gtgcggcgat aggtggcata ctcctcaccc cggctcgtca tggtctgtgc ttcagtccag
    52921 ggaatgccgg tgactttcag gagtgcccag cccatcgtca gcgggccaat cagcgtcaac
    52981 gcccagttgc ccagcggact cgccagtccc atcaccacgt agctccacca atgcagccat
    53041 tcaaaaaagt aatttgggtg tcgcgagtag taccacagcc cttcccggca cacacggtcc
    53101 cggttccatg gctgacgccg aaaggatgcc aactgccaat cggccatggc ctcccctgtc
    53161 acggcagcca tccagagaag gaccccgagt agctccatga gatgaaacgg cggatgagga
    53221 ttctgcatga cgatcagcgg cggaagcgag aaccagacga tggccgcggc ctgcaactga
    53281 aaataccaga accggcgtag cggaccctgc tcgccccatt gcacacgcag ggcgcggtag
    53341 cggccgtctt ctgatttgtc ccacagtcga tcgatgagga catgcgtgcc gaggcgtgcg
    53401 gcgtagagca cgatcatccc gcacaccaac agacgcctcg ccggttcgcc aggaacagca
    53461 gtggtgtacc acagcacgac caaggccaac ccgtaacacc agcccacatc ggcgagagca
    53521 agattgtgca ggcggcgctc gagcaaccag agcgcaacca tcaagagcgc tgaagcggcc
    53581 caagcggtca tgagaaggga gagggggtct gtcgccgtca tgcgatcagt gatcgacgtg
    53641 aaatgcaaag atacatcacg tgtggttcgt gttcagatga ttgtactacg acggacatcg
    53701 cgacacacgg aggaggagat cgcatggcat tgccaccgca gcgggcgtat gattgctcat
    53761 atcagagcaa gggtgcttga tacacaggag gaacagagat gaagggcgaa catcccgtta
    53821 cgatggccat cgtcatggcc gtcgggacag tcttgatgca ggcggacgtg cacggtgaag
    53881 cggcaggaaa ggccgatgcc ctggcaaagg ggaaacaggt gttcacgagg cattgcgccg
    53941 ggtgtcatgg tgcagagggg aaaggcgacg ggtacaaact actcggcccg gatccggcca
    54001 atctgaccgc tcccgcgacc aggaagcaat cagacaaggc gttgctcacc accattcacg
    54061 aaggcaaacc gaacatgcct tcgtggaaag ggcttctctc cgagcgcgac atcacgagcg
    54121 tgctggccta catccgttcg ctgccgcact gatttcgcct caatctagcg ggcagcggga
    54181 taatcccacg gatcgccact ctccgacgga tgctccgtca gccactggcg aaatcccggc
    54241 agagggaacg atagcagtgc ctggatcttc tcactgcaca tcgagagatc cggcggacgc
    54301 ggcggcccct gataatctgc caccgaaccg ggctggatcg aagaccgcaa ttccgggaat
    54361 cgcatcgcca ccagctctcc gatctcccac ctagagagcc gctcatggcc acccagatga
    54421 tacagacccg gccggcgctg catgccgagc tcccagagcg cccgcaccag ggcacccgcc
    54481 gggatcggac agcgaaactc gtctgtaaac agtgtgagtg tcaccccctt cgatgccgtg
    54541 cgcaccatgt cctcgacgaa actccggtcg cgcgtcggtg acattcccgc ggtcagcgca
    54601 atacgcacga ctgtatgggc cggattggcg aggacgacct gctccgcctc ggccttcgtc
    54661 tgtgcgtaga cgtttaacgg atggaccgca tcggtttcga catactgccc cttcgcgcca
    54721 tcaaacactt ggtcggtgga aagaaagatg aagggcacgt ctcgcgcgag gtcggccagg
    54781 agccgcgtgg cctcgacatt ggtcaacctg gcccgtacgg gatcctgttc gcagagaccg
    54841 atgcggctca aggccgcgca atggacgacg agagccgggc ggtggcgatt ccaaagctgt
    54901 tccacctggg ctcgatccgt gaaatcggcc tcggccctcg tcacgcctac cacctcccaa
    54961 tcaggcaccc agcgcgaggc actcctgacg aggtagccac caatcaaacc cgctgcaccg
    55021 gtgatgagtg cgatccgttt catggttcgc tccgccccag gcgccggatc tccggcacgt
    55081 cgatgaccct ggtcttcacc ggaggctgct cgacagacca cagcagggcc gcgaccatct
    55141 ctcgaatcgt caccaaaccc aaccgcatgg cggcctccct cgtcgcgggc actcgttcca
    55201 gcatacgata gaccggcaac agcgccaatg gccaccaatg tccgggaccg agaatgtacc
    55261 agggcctcag aatcgtggcg accaatccgg ctcgcctgag agcctcttca cagtcacgac
    55321 gtacggcgat gtagtcacgc atgatgggcg ccggatgggc cacgctgacg tagacatagt
    55381 gctgcacgcc cgtcgaagcg gcggcgtgaa ttgcggccat ggccgagggc ccatcaatcg
    55441 cgcgaaactg cggccccttc cagggtgatg gtttgggtac gcccaccagc tgcacgagtg
    55501 tatcgtgtcc ccgcacagat tccgcgaagg actccgcatc cagtggattg ccgaccacaa
    55561 cccggcaccc gacgacaggc tttcctgcag aggactcgcg aaccaacgcc gtcacgtcat
    55621 gccctcgctc gagcagcaac ggaatgagcc gcgccccgac gtatccggtg gcgcccgtga
    55681 tgaaaatgcg atgcttgtcc gtcggcctag tcatggatgc gagaacagcg ccggcttggt
    55741 ttccaggaaa ccggcccacc cggccattcc attcagctcc tcgcggccct tcgcgacgag
    55801 gcgagctgcc gcggtaatca acgcagcctg atcctcagtc gataacccga ggttcgtttt
    55861 gatatgcgta acgtggtctc ccaatggatc ggtgtccggc aactggcgca gtgcgacatg
    55921 ccagaagcgg caggatcctc cgccaccgtt ctcgcccact cgaaaggtgc cgaactgggc
    55981 cacatcctgc cgggatgcag gaatgccggc cagctccaac acattgcgac gatgccctcg
    56041 caagagggcc gcggcggcgg agagcggcgt gtgcgtctca ttcctctggc ggcccgtcgc
    56101 atcgatggag atgacgagac aaccttgcgg aaagagatcc gtcatgggac ggttgactaa
    56161 cgctcgctcg acgacctgca tgagagtccg aatccccaaa ttgtcagccg cgcccccatc
    56221 ataggcgatg atgggatcgg cggacgcgcc gttcgttgcg gtacttccat gggtaagctt
    56281 caggggctcc agcacgccag ggtaggccgc agacatatac acggcccggg caatgctgaa
    56341 cggcgccaag gggatgccca acggtgcaaa tcgttcctcc gcaatcacca ggggatcgcc
    56401 ggtcagggca tccgtggagt tcaagagcaa gaggggtcgg gagggattga ggtccgcgaa
    56461 cgtggcaccg cgaaacagtt ggtcgtccag agcacggatc accggttcgg cggggatcgt
    56521 gtccttgaag gcgtaccgga ccagattgtg gggagccgca taccagggcc ccgccactgc
    56581 gccttgaata tcgcgcccga tctgctccac aaacccgtct tggaaccgaa agtctccgaa
    56641 cccttccaat gcataggatg cagcgggcag cgcgccgccg gagaccgccg aaagcacatc
    56701 gacctgcgat aacaggccgg cccgctccag ctccttcatg acggccgccc cgaagacggc
    56761 cgcgcgactg cctccgccgg agaacgccag tccgacaaac cgtccatccg ccaattcctt
    56821 ctgccccaat tgcagcgtgg cccttgtggg tgcaggacga tggtcaggcg ggagggcccg
    56881 gggagcatca atggtcgtgc aggaggcgga agccagggca agcaccgcca accaacggct
    56941 tagagtacgt ccccatatca tgctcatgga tgaatctacg gccgaatgcg gaaaatggat
    57001 gtgcttctag taaggagtcc tggcgttgag gcggacgcta acaggatgct gacaccatac
    57061 aggcctacag caactatgtg actctcaaag actcagagac gtgcgcagtg gccgtggcag
    57121 gttgttcaaa aaggctgtcc ggcaaggccg cagccagcgt agaggcgatg cgtacccttt
    57181 gcggtacgtg gagcctcttc gcgccgcgag aacgccgccg gcagcccttt ttcaacagcc
    57241 ggctagcccg catgattcat gacggttcgt cgcgaaatcg ccgagtcacg tggcgagacc
    57301 ggcgcgcgga gcccgggagg acccgaagcg tactcgtgca gtacgttgag ggtccgaggg
    57361 gcgagcccgc tggccgaagg ctcgtcgtag cagtgagttg gcgattgcag cagaagtgct
    57421 catgaatcat gcgggctagt tcctggtcag cttgcggtac cgaatccgat gcggctggtc
    57481 ggcttctttg ccgagccggc gcttccgatc ggcttcgtac tcgctatagt tgccctcgta
    57541 ccacaccact ttgctgtcgc cctcgaaggc catgatatgc gtggcgatgc ggtccaggaa
    57601 ccagcggtcg tggctgctga tgacggcgca accggcaaac ccttccaatc cctcttccag
    57661 agcgcgaagc gtgttcacgt cgaggtcgtt ggtcggctcg tcgaggatga tcaggttcgc
    57721 gccctctttc agcatgcgag ccaggtgcac acgattgcgc tctccacccg agaggtcctt
    57781 cacttttttc tgctggtcgg tcccggcgaa gttgaagcgg gcgcagtagc cgcgggcatt
    57841 gacctcggcc ttccctagca tcaccgtgtc ctgcccgtcc gaaataattt cgtagacggt
    57901 tttgttggga tccagcgatc ggtcctggtc cacgtaaccc agcttcaccg tctcgccgat
    57961 gcgaatggtg ccgctgtccg gtttttcctt gccgatgatc atgcggaaca tggtcgtctt
    58021 gccggcgccg ttcgggccga tcacgccgac aatgccacct ttcggcaggc tgaattccac
    58081 gttctcatac aacacattgt cgccgaacgc cttgctgatc cccttggcct ccaccacgac
    58141 gtcgcccagg cgcggtcccg gcggaatata aatctccagt tcggccgcca gctgatcctg
    58201 cttttgattg accagttctt cataacgatt gaggcgcgcc ttaccctttg attgacgggc
    58261 tttcggtgac atccggatcc attccaactc gtgctccagc gtcttcttgc gtttcgattc
    58321 agccttctct tccttctcca gccgctcctg tttctgctcc agccaagacg tgtagttgcc
    58381 ctgaaacgga atgccgtgcc cgcggtccaa ttccagaatc cagcccgcca cgttgtcgag
    58441 aaagtaccga tcgtgcgtca ctgcgatgac ggtgcctttg tattgctgca gatgctgctc
    58501 caaccattgc accgattcgg catcgagatg gttggtcggc tcatcgagta acagaatatc
    58561 cggctcctgg atcatgaggc ggcacagggc cacccgccgc ttttcgcctc cggagaggac
    58621 gctcactttt tgatcggcag gcggacaacg cagggcgtcc atggcgattt ccaactcgct
    58681 ttcgagttcc catccattcg ccgcctcgat cttctcttgc agttgcgcct gcttgtcgat
    58741 gagcttttcc atctcatccg ggctcgcgtc ccccatgctg ttgctgaccg cttcatattc
    58801 gtgcaacaag gcgacgagtt ctttcttgcc ttcctccacg acttccttga cggttttgtt
    58861 cgggtcgagc tgcggctcct gttcgagcaa accgacgcta tagcccttcg agcgtgtgat
    58921 ctcgcccaca taattcggat ccaccccggc gatgattttc aacagcgagc ttttgccgga
    58981 accgttcaat ccgaggacgc cgatcttggc gccgtaatag aaacccaggt agatatcgcg
    59041 caggacttgt ttctttggcg gatagacctt gcccactccg accagtgaaa aaataacttg
    59101 cttatcgttc gtcgccatag ccatcctcag tagagtgtga accgtctgtt cgatcgtgca
    59161 atcgatgacc ataacaaagc gcggcattat tcacaaccgc gcgagggagc tccgggcatg
    59221 attccagacg cacggattca gcgaagatct tgccgcatca tcctggcact gctcctgcca
    59281 ttcctcgcct ggactggtgt cgcctccgcc cttgcagacg atcgcgagca gcgcgacctg
    59341 gtggaacagt cgcgcatgac cctgtcgaac ttcctcgccg attccaacat gacctggttt
    59401 cgcgaccacg tcccagaggc caagggtctg ttcatcgtgc cgcaatatat gaagggcgca
    59461 ttgatctatg gcgcagccgg cggcagcggg gtcttcgtcg cgaaggacga caagaccggc
    59521 gaatggagcg agccggcctt cttcaccatg ggggccgcca gcttcggctt tcaattcggc
    59581 gcgcagatgt cggaggtcgt gttgctggtt ctgacacaac gcggggtaga ttcgttgctg
    59641 ctggggaatt tcaaactagg ggcagatgga tcggtcgccg tcggaccggt cggcgcgggt
    59701 gtttccggcg cgacgacgcc caacctcagt gcgga
//
LOCUS       DAOQDS010000017        59184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_156_length_59184_cov_21.430533, whole genome shotgun sequence.
ACCESSION   DAOQDS010000017 DAOQDS010000000
VERSION     DAOQDS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 59184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59184
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156_length_59184_cov_21.430533"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /gene="hflC"
                     /locus_tag="PK589_05905"
     CDS             <1..444
                     /gene="hflC"
                     /locus_tag="PK589_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNJ19166.1"
                     /translation="ARAQLMAVVTQRANEKASVYGIEIQDVRIKRADLPEQNEKAVFS
                     RMQAERERQAKQYRAEGAEEAQKIKSEAEKDREIILAEAYRESEELRGGGDAKAFKIY
                     ADAYRQDPHFFEFTRTMEAYRKTLKDKTTILVSPDSEYFRFLKQR"
     gene            complement(451..>2067)
                     /locus_tag="PK589_05910"
     CDS             complement(451..>2067)
                     /locus_tag="PK589_05910"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNJ19167.1"
                     /translation="GIPSEDLIPYGRYKAKIAANFSARVKSRPLGRYILVTAINPTPL
                     GEGKTTTSVGLSMGLCRLGHNAAVTLRQPSLGPVFGIKGGGTGGGRAQVWPMEDINLH
                     FTGDAHAVSASHNLLSAFVDNHLFQGNALKLDPQRIRWPRTLGVSDRALRDIVLNAEA
                     GPRPGQFVITEASEIMAVLALATDQRDLRQRLGRILVGLTESGAMTTAEAFGCAGSMS
                     VLLKDALLPNLVQTLEGTPAFVHAGPFGNIAHGNCSVVSDQLALRCADYVITEAGFGS
                     DLGAEKFFNIKCRTSGLRPDAGVVVATLRALKLHGGGGIVKAGTPLPPGLTEVNPAAL
                     EKGFANLEQHIANVRAHGVPVVVAVNAFKDDSPKELEWVCERALAAGAAAAAVSTHWA
                     DGGKGAEELARAVVKVAAQGSQFRPLYDATWPIKRKIETIATTIYGAAAVSYSSQAEK
                     DIELAARLGFEQLPICMAKTPLSLSHDPALKGRPSGFTVPIQELRILSGAGFLTAVCS
                     GIQLMPGLPKKPAGERIDIDPQSGEIVGLS"
     assembly_gap    2068..2167
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2168..3186)
                     /locus_tag="PK589_05915"
     CDS             complement(<2168..3186)
                     /locus_tag="PK589_05915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNJ19168.1"
                     /translation="MSQQTAETSDAYTVVVFRGSSSKPLRFSFPRKFVRKLLILAAIL
                     IVADLLVVSHYVIRTGEVWQLSAFRAEAMGAREQTAAFSAAIDDLKKRLSAMGEVNQR
                     LRVMLGIDTTKPAGDLANGRGGEDGPLPDGKSSVPGNGPASSGLESRQQVSELRENNQ
                     SDLDFSTESIEEATQQVRESLEALVREATQQEEALQSLTQVAEQRSTQWASTPSIWPV
                     RGWVTSGFGPRVSPFTEKPAWHDGLDIGAQANSPVQAPALGRVVTVAFDSKMGNMVKL
                     DHGYGIETVYGHLAKSLVKEGQRVKRGDVVALVGSTGLSTGPHLHYMVKKNGQALDPT
                     KFILD"
     gene            complement(3305..3661)
                     /gene="cutA"
                     /locus_tag="PK589_05920"
     CDS             complement(3305..3661)
                     /gene="cutA"
                     /locus_tag="PK589_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNJ19169.1"
                     /translation="MQIALAGGGVDAQGSEVVVFITASSNEEAEKLGRMVVESRLAAC
                     ANIVSGIRSIFRWDNQVTVENECLMIMKTTLQRYSELEAVIRQHHSYTVPEIIALPIV
                     AGSVSYLDWIRSETAK"
     gene            3982..4302
                     /locus_tag="PK589_05925"
     CDS             3982..4302
                     /locus_tag="PK589_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNJ19170.1"
                     /translation="MSFTITPTELKSRLDKGDKLVLVDVREPWEYAIAKLEGSVLVPL
                     ATLQQSLGKLDRNSEIIAVCHHGMRSADATGFLLQQGFGNVKNLIGGIDAWSVQVDSS
                     VPRY"
     gene            complement(4312..5235)
                     /locus_tag="PK589_05930"
     CDS             complement(4312..5235)
                     /locus_tag="PK589_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ19171.1"
                     /translation="MKVLVTGGAGFIGSHVVDRLLQEGHDVVVVDNLVTGKRKNVPKA
                     AQFYKLDIENPKLERVFRNERPSVVFHLAAQMNVRRSVEDPLFDAQVNVLGTLNVLEQ
                     ASKHGARKVIFSSSGGAIYGEQLAFPAPETHVTQPLSPYGISKLCGEHYLSYYHRMSG
                     IQVVSLRYANVYGPRQDPEGEAGVVAIFIQKMLRGEQAVVNGNGRQTRDFVFVEDVVE
                     ANLMAMGPQVEGVYNVGTGIETSVNDLFRIVVDLTKVEFKEVHGPAKRGEQARSVIDC
                     AKLQRTLGWEPKVELREGLRRTVEFFRDGLG"
     gene            complement(5395..6150)
                     /locus_tag="PK589_05935"
     CDS             complement(5395..6150)
                     /locus_tag="PK589_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNJ19172.1"
                     /translation="MTQKPRPERERLDRALVSRGLVSSREDAARLILAGLVRVDGIVV
                     DKAAKPTMPGALVEVTGPGSPYVGRGGEKLAGALNQFQVDPSGMVCFDVGCSTGGFTD
                     CLLQRGATRVYAVDVGYGQFEWRLRQDPRVVLMERTNVRYLEPGAIAEPIDLIVIDVS
                     FISLTLVLPAVVSHLAASGSIITLIKPQFEVGKGLVGRGGIVRDDALRESTVEKVVSC
                     AVQLGLQLVGRMDSPIEGRKGNREFLAWFQRKA"
     gene            complement(6215..6463)
                     /gene="xseB"
                     /locus_tag="PK589_05940"
     CDS             complement(6215..6463)
                     /gene="xseB"
                     /locus_tag="PK589_05940"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNJ19173.1"
                     /translation="MAAVKFEYAMARLETIVAELEKGDLPLDDSLKIFEEGIRLSKTC
                     LKMLEDAERKVEILVQEKDGKKRIQAFSPGEDELENPQ"
     gene            complement(6523..7881)
                     /gene="xseA"
                     /locus_tag="PK589_05945"
     CDS             complement(6523..7881)
                     /gene="xseA"
                     /locus_tag="PK589_05945"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNJ19174.1"
                     /translation="MTGHNLALPLLLSVSDVTRLIRESLEEQFQDVWIEGEISNLRAP
                     SSGHLYFTLKDSQSQLRGVLFRSGALRLRFALQEGLSVIARGRISVYEARGEYQLIVD
                     SLDPKGVGAFQLAFEQLKERLSQEGLFDEARKRRLPSFPSTVGVVTSLTGAAIRDIVA
                     VLRRRCPTVNILIAPVPVQGEGAGASIAEAIDVLSEMPQVEVLIVGRGGGASEDLWAF
                     NEEAVVRAIARSRVPVVSAVGHEIDVTLSDFAADHRAPTPSAAAEAVVPVLAEIMERL
                     DELARRLRRLVDTMLQMQRHRFERSLSVLGETRFRIQAQAQRLDELQDGLTRALTAQL
                     TVLQRELVERRHLLLAQGPRNRIERFLVVIPQLCKRLEQEARRGLMSRRQSVAMQMAA
                     LDALSPLAILQRGFSIVHTISNRKVLRRASEVTVGEAVEVTLAEGQLVCQVEKVMPSS
                     IS"
     gene            complement(7919..8698)
                     /locus_tag="PK589_05950"
     CDS             complement(7919..8698)
                     /locus_tag="PK589_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNJ19175.1"
                     /translation="MKVLMIGDIMGEPGRRSVARLLPKLIANHSIDVVVGNGENVAGG
                     FGITPDLVDDLFDLGVSVITTGNHAWDKKEILDVFPREPRLLRPANYPAGVPGRGSYI
                     FTTPGGESLGVLHLMGRAFMPTIDCPFQVAKREVERLKSQVSAIVVDMHAEASSEKMA
                     MGHYLDGLVTVVAGTHTHVQTADEQILPKGTAYITDIGMTGPLHSVIGIKKELAIEKF
                     LTGMPRRFEVASGPTVFCAVLIDLDATLGKAVSIERIRVMD"
     gene            complement(8709..10286)
                     /gene="rny"
                     /locus_tag="PK589_05955"
     CDS             complement(8709..10286)
                     /gene="rny"
                     /locus_tag="PK589_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNJ19176.1"
                     /translation="MVPISLSVVAYIFIGLVGAVLGAGLYEVLRRRSAITRRAEAEDQ
                     STQIVQSAQREAENLVKEAKLEAKDLVFQARIELEKEQKAKLAEVSNVERRVSQREEG
                     LDRKVSLLEKRDQDVLKREQDLLKREEVLSQKDAACAQALKQHREALERVAGLTAEEA
                     KRQLIQEMDSQARLEAAGLAKRLLEEAKENADREAREIIASSIQRVTRDYVNEATISV
                     VPIANDAMKGRIIGREGRNIRAIEAATGIDLIIDETPEAVIISGFDPLRREIAKVSLE
                     RLMHDGRIHPTRIEEIVEKVKVDIEKLMIEEAEKVIFEVGLSDFHPELVKVLGRLKYR
                     TSYGQNNLYHAREAAYICGIMASELKLDVKLAKRGALLHDIGKAVSHEEEGPHAMLGA
                     EIAKKYGENAKVVNAIAAHHEQVEPICPETVLVAAAEALSAARPGARREALESYVKRL
                     EKLESLATVHKGVQKAYAIQAGREIRVIVKQEDLSDPECFQLSRDLAKKIEQELTYPG
                     QIKVTVIRESRFVDFAK"
     gene            complement(10834..11130)
                     /locus_tag="PK589_05960"
     CDS             complement(10834..11130)
                     /locus_tag="PK589_05960"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNJ19177.1"
                     /translation="MTKTIDVEIYGQRYTVNGEADESYVKQLAEIVDKQMKQVAAGMR
                     SATPAKLAVLAAFNLAHELMESERRFRQDEADADRRVASLMESIDQQMPSILSR"
     gene            complement(11123..11377)
                     /gene="zapB"
                     /locus_tag="PK589_05965"
     CDS             complement(11123..11377)
                     /gene="zapB"
                     /locus_tag="PK589_05965"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HNJ19178.1"
                     /translation="MTLDRLDALEIRIRDLVKLVQDLKRKNTSLEDELRLARERVAVR
                     DDENRRWEQERLDIRSRIEKVLGEIDLLECLDEPKEVAFD"
     gene            complement(11472..13637)
                     /locus_tag="PK589_05970"
     CDS             complement(11472..13637)
                     /locus_tag="PK589_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNJ19179.1"
                     /translation="MTAIAESPRPLPVLPPLCVSAEDCFRSALAVNDRTGSPAQREQA
                     TALKIDQLRSVMELFPSTPWAPRARVVLGVLLIEREPAEATKLLRAAQPELPVIDDYF
                     RLWIAESLSKQNEPIQAAELLETIPKIVPDSNLIAKAAYRTGEAWYSANVCFRAVDWL
                     ERAVALADKDQAAPMALSHQADCHLRENRLPEARTALKQLWLRYPQSPEARDAKTRLE
                     GGLGGESWLPTAEEYLIRAQAFLGLSMQAEAVEELRRFLSLAPGHARRHEARLKLGVA
                     YVRLKQYDQARETFRGLVADRVQESAEATVWLARVYLRQSLGDKLIELARSAAQGPLA
                     GDQRAMVHLFAGVWLEDQGKFDEAIGMFRQVAKLGDSASQRAEGLWRAGWAQYRTARY
                     KDAAETFRAVVELHVNGFEPQAMYWAARAEEHGKNANVGDQYARVCQRHAFSYYCQLA
                     ARRTSIPANQPAVGSVERPAGEEIERLPENRRPEIEKHAVYQRGIELKILGFAQEAAR
                     ELGALTEQYSRDQDVLLAFSTMLSEVGAYHPALRVAKVHFREKLERSGLPTAPALWTV
                     AYPTGLVPTIAAQGVTGVDPYLAAAIIREESQYDEKAVSVVGAVGLMQLMPVTANAVA
                     QRYGFPTVGREDLFDQETNIRLGVRYLGLLIEQYSGNLAHAVAAYNAGPIAVNNWIAV
                     HRGRDQDEFVELIPYQETRLYVKRVLRSYGEYRRLHGAS"
     gene            13816..14904
                     /gene="rlmN"
                     /locus_tag="PK589_05975"
     CDS             13816..14904
                     /gene="rlmN"
                     /locus_tag="PK589_05975"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNJ19180.1"
                     /translation="MLSHTATRHNLLALTESDMAAFVASLGWPAYRASQILRWLYQER
                     VRTFADMSNLSQKDREHLTSTCSIERATDVHIFASQDGTKKFVLTLTDGKQIECVLIP
                     DEDRLTLCLSTQVGCTLDCGFCLTGTMGLTRNLRAHEILDQVLTAQDHLEADQRLTNL
                     VFMGMGEPLANLDAVADAVTRLTNQTWGLGLSGHRITISTAGLASRIKEVAPLKVNLA
                     ISLNATTDELRHQIMPAANRLHSLQALLAACRDYPLAERDRLTFEYVLLADVNDRPED
                     ATRLIKLLRGLRCKVNLIAFNPFPGSPYRRPSDAAIDQFQNILRRGHVDAYLRRSRGR
                     DVLGACGQLGRIEMPQVPVTLTQIQTRC"
     gene            14919..16313
                     /locus_tag="PK589_05980"
     CDS             14919..16313
                     /locus_tag="PK589_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ19181.1"
                     /translation="MPKLSTSHRSSLLRHSVFACSLLSLLLFTSQSSTAHAVEPKEFT
                     LSNDMKVILVEVPKAPVATVQVWYKVGSRNEVMGRAGLSHMLEHMMFKGTAKYPKGTF
                     SRLIRKNGGMDNAFTSQDFTAYFENLAADRVTLALELEADRMQGLILDANEFKTEREV
                     VKEERRLRNEDDPQGALVEALFAQAFMSHPYHWPVIGWFSDLDAMSLDDLQRHYDTYY
                     SPNNATLLVVGDIKAETLLPVIARLFEPIPKGPSPKALTVTEGAQHGERRFLLKREAQ
                     VPFVMMGYRVPNYTSDDSYALNVLESILSHGKSARLYQSLVYEQKTALAVGADYGLMQ
                     ADPGLFYFYAVVKPGEKVEAVEDAVLKEIQRLQTEPPSELELQRAKNQIEAAHIFEQD
                     SNFRQAMLLGEAETIGAGWRKVSQFVERTRAVTPQDVQRVASQYLAADMRTTGTLIPL
                     PPQTQSASPAQPHQ"
     gene            16322..17695
                     /locus_tag="PK589_05985"
     CDS             16322..17695
                     /locus_tag="PK589_05985"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ19182.1"
                     /translation="MMRRQVPHSPRRHRSPGRFFLGLLLLFASLPSTQAQAADIVPSR
                     SVTANGMTVLFLEQHFLPTVEIHALVKVGSAQDPPDKAGLANLTASLLDEGTQTRTSR
                     QIAEQIDFVGGSLEAHAAEDFTTASARVLKKDADLGFALLADVLQHPAFHKQEFERVR
                     TQILGEILSDDDDPGNVAMKAFHQLIFHGHPYSWPAHGAEDTLNKITVADVQQFHARE
                     YLPNQTILVIVGDLTQEQAATLVQTHFGSWKKGAPSPYQLKKPTPIDRKMVQLIEKDL
                     TQSTIVLGHTGISRTNPDYYAVTVMNYILGAGGFSSRLMDSIRDKQGLAYGIMSQFDS
                     RLLPGAFYISLQTRTEVTNQAITGVLTEIKGIRDAPVTDQEINEAKSFIVGSFPLRVD
                     SSAKLANVLAQVEFYNLGLDYFTNYPKAIEKVTKDDVLRVAKQYLDPQHYALVVVGSI
                     AKAKVKQ"
     gene            17743..18573
                     /gene="larE"
                     /locus_tag="PK589_05990"
     CDS             17743..18573
                     /gene="larE"
                     /locus_tag="PK589_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNJ19183.1"
                     /translation="MVSSTPLIEKISRARHLLREMGSVIVAFSGGIDSTLVLKLAHDE
                     LGMQATGVTAVSPTLPASELALTQQLATEIGARHRVVETDQLEIPQFVRNDATRCYHC
                     KTDLYSLLGKLRSEDATACIVDGTNVDDLGDDRPGLKAAREHGVRSPLLEAGFTKADI
                     REAARELHLSNWDKPAAACLSSRVPRGITITRSTLSRVEQAEAALIQGGFRHCRVRDY
                     GDLARIELAVEELPKLLEAGCREQLVESLKQIGYRFVTLDLEGYRQGGVSLTPPASET
                     "
     gene            complement(18570..19082)
                     /gene="atpF"
                     /locus_tag="PK589_05995"
     CDS             complement(18570..19082)
                     /gene="atpF"
                     /locus_tag="PK589_05995"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNJ19184.1"
                     /translation="MPQFESHFFSSLIFWEILSFGILFFLLYKYAFPSLLGMLEEREK
                     KIKDSLDQAERHRSEAERKLKEYEAKLATAAKDAEALLAQAKERAARLMEENEQRMTA
                     DAERIKGDATREIETERRKAIQDIRSQTTDLAMMVAEKVVGRALTDGDHRRLADEALD
                     ALAKTYQSRN"
     gene            complement(19156..19386)
                     /gene="atpE"
                     /locus_tag="PK589_06000"
     CDS             complement(19156..19386)
                     /gene="atpE"
                     /locus_tag="PK589_06000"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNJ19185.1"
                     /translation="MDAAAAALVGMGLAAAGFAGAGVGIGYIFGKMIEAVARQPEAEG
                     RVGKYMWIGFALVEAIALYGLVIAFIIMGLRK"
     gene            complement(19422..20171)
                     /locus_tag="PK589_06005"
     CDS             complement(19422..20171)
                     /locus_tag="PK589_06005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNJ19186.1"
                     /translation="MEESPLHAFELHDLIPLVPAGVDISINKAVILMWVVVGLVAFLM
                     ISAAAARKLVPGKLQNMAELIVEFIRGIILDTMGEAGMKYFPLIATLFLFILFANLLG
                     LIPGSYTVTSQIIVTAVFAVGIYGLSLVLGFSLHGMKFLGILVPPGTPGWLLPLMIPI
                     ELISQLARPISLAVRLFANMTAGHVILGVLFGLAISGGLLIGWLPFAFTIAMNGLEVG
                     IAFIQAYIFTVLSCVYLGDAVTLHGHSEHAH"
     gene            complement(20202..20420)
                     /locus_tag="PK589_06010"
     CDS             complement(20202..20420)
                     /locus_tag="PK589_06010"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNJ19187.1"
                     /translation="MPPSQDPLYAGLGQAVRIGTELLAALIVGGGLGWVVDTYLLDSN
                     PWGLVAGLGLGAAAGIRNAYRSAQRWQS"
     gene            20593..22077
                     /locus_tag="PK589_06015"
     CDS             20593..22077
                     /locus_tag="PK589_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNJ19188.1"
                     /translation="MTAVSHQAFLNGPPQPLVVVRPLPNADAFELYQRLGITDGPSFL
                     LESGNGTNATARYSFFGNHPYLTLTGRAQDYSMKADGHITTAHGYAFDAFRQAFADST
                     IPQPDGLPPFYGGAVGYLSYDLVRSFESLPTLASDDLGLPDLHMAFFDTVAAVDHHTD
                     QLYLMYCPPLSRFRAEPREKLLREGSDRLAELEARLTSISRPHPAPTGPIQTSFVPSQ
                     SRDAYTARVRRCQDYIAAGDIYQANLSHRFTLNLDASWSAADRDRYAGILYSRLRRVN
                     PSPFAGLVRFPELTLVSSSPERLVRLTASQASTRPIAGTRPRGNGESHDVTMRAELLA
                     SPKERAEHVMLVDLERNDLGKVCQYGSVRVDEFMTIEQYSHVSHLVSEVVGTLRPGTS
                     PFDLVRAVFPGGTITGVPKLRCMEIIDELEPVRRGLYTGALGYFSWSGDLDLNILIRT
                     LVLTKDRGYLQVGAGIVADSDPDREYDETLAKAGAFLHILEADR"
     gene            22077..22958
                     /locus_tag="PK589_06020"
     CDS             22077..22958
                     /locus_tag="PK589_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNJ19189.1"
                     /translation="MWVFLNDRFVPKEQAVVSVFDHGFLYGDGVYETLRSYGSRIFMR
                     DQHLARLRRSAEAIGLTIPIPDAQWPDLLHEAMRRNEVGNDRVDAYLRITISRGEGEI
                     GLDPALCPRPTVVILTKVLHPSPPALSRDGVTLTVANTRRNLPEALSPHIKSTNFLNN
                     ILAKRESIAAGTFDSLFLNWREELTECTISNLFFISNGTVHTPSLDCGILDGITRAIV
                     MTLAEEEGLPVQEGHYHLADLRQADECFLTNTSMEVMPVSRVDAWPIGDGRPGPLTRR
                     LQARFAASRTRFLAPAP"
     gene            23109..23774
                     /locus_tag="PK589_06025"
     CDS             23109..23774
                     /locus_tag="PK589_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNJ19190.1"
                     /translation="MARFKRTTPLLLLYATLLLTHAPVQAEVYQYIDANGTISLTNVP
                     NDPRYKRVMSELPRSRSVISDRELDPVIARHSRAHRLHPALIRAVIKTESDFDPLAVS
                     HAGAVGLMQLMPQTAMRFDVRDSYNPDENIGGGTKYLRQLLDRFNGNLPLALAAYNAG
                     EHAVERYQGLPPIPETRQYVVKVLGYYRTFLMNARTSSSRAYVPQAPRSQGRGLAHST
                     ASR"
     gene            complement(23680..25356)
                     /gene="nadB"
                     /locus_tag="PK589_06030"
     CDS             complement(23680..25356)
                     /gene="nadB"
                     /locus_tag="PK589_06030"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNJ19191.1"
                     /translation="MSARSASPKIETDFLVVGSGVAGLRAAIELSRAGRVLMLTKGHP
                     LESNSMYAQGGVAVALSEEDDVGSHLTDTLKAGHGLCRREAVRVLVEEGPERIQELIA
                     WGAKFDKIGKRFAFTREAAHSRSRILRARGDATGNEMVRALMTYAARQPRIQRLDRRF
                     TVDLAVVDGRCCGAIVLNEMSGERTVLPASAVVLSTGGAGQVYARTTNPGNATGDGMA
                     MALRAGALLMDMEFVQFHPTSLYLPSSPPFLLSEAIRGEGGQLRNIKGELFMHRYHPD
                     GALAPRDVVSRAIWSEMAATRARHVYLDVTHLGAAFVKRRFPTIYATCLRYDIDMTEE
                     WIPVSPSAHYMMGGVWTDVHGATTLPGLYAAGEVACSGVHGANRLASNSLLEGLVFGM
                     RAAQSAVAHAGHFSESVQLPSLEELEQGRPTELDDPEKLRSSLRRLMWGKVGLVRSGD
                     SLISASAQLVRWMRLLGKPFSSRAALEVKNMVQVARCITDAALWRENSVGAHYRSDFP
                     EAKRQGWRNHSQLLLGGGQVNGMPSNERDLVLATEAPVGRKPGKKTSGRS"
     gene            complement(25359..25874)
                     /locus_tag="PK589_06035"
     CDS             complement(25359..25874)
                     /locus_tag="PK589_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19192.1"
                     /translation="MFTSAARRSPWIGRLLLGFVLILGVSCSGKTIQYKDDHDRILRI
                     DQAVESLRKSYVERDRSALEALLLPSGGLDQVQRDIHSDFDTFRDIQLEFSIERILIE
                     GDNIDVFVHWQGQWKRDPGDVGTRQRGHGRLAWVGTQSILLREAQGDLPFGMGTRASL
                     NEDPARSQPKP"
     gene            26174..26392
                     /locus_tag="PK589_06040"
     CDS             26174..26392
                     /locus_tag="PK589_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19193.1"
                     /translation="MPEGKKVRIRVRTVNCIYVGDFLIPPMRNRVSDAINEEQRLFIS
                     LTDVLINDKDRSDFVAINKNLIESIAQL"
     gene            26493..28223
                     /locus_tag="PK589_06045"
     CDS             26493..28223
                     /locus_tag="PK589_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ19194.1"
                     /translation="MFSFKRFYPFLKPYVPRMIAAAVMVMAVAAINLALLRLGGTLWD
                     VITVQHDAPRMTNMILLLLGLVLIQGLCSMGHSYLTAWVSQRVMADFRIHLFAHLETL
                     SVNFFSKRRTGELMSRLMNDVTVIQNVVTDTPIDAAKQIVTFIGGAGFLFAMNWQLCL
                     LILVLLPMLVVVAKLFGRRLRALSTKIQDQTATVSTLVEEVIAGIRVVKSFVQTKREE
                     ERFVTQVQSAMELSLRRATIMAWFVPTIIFVTFAAAALVLWYGGRQVIDGSVSPGDLF
                     AFVLFAGILIGPFGSAARVFAQIKEAQGAMQRVFEILDTHAEIADAPGAIELPAVRGH
                     VRAENLSFAYDPRQPVLSDVSFEAKPGELIAIVGPTGSGKTTIMNLLHRFYDPTSGRL
                     TIDGHDVKNVRLDSLYRQIALVPQDTILFGGPIRDNIRYGREDATEEEIIAASTAAHA
                     HEFIVGFPDGYQTIVGEKGINLSGGQRQRVAIARAILKNPRILLLDEATSALDTESER
                     LVQEALERLMVNRTTFVVAHRLSTIQRADRILVLNKGKIVESGTHAALLEQQGLYHYL
                     YTLRLSELPV"
     gene            28241..29113
                     /locus_tag="PK589_06050"
     CDS             28241..29113
                     /locus_tag="PK589_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNJ19195.1"
                     /translation="MNRSVFALVFTALFLSWTTPGSAHSPLPKPDHVVIVIEENHAFG
                     QIIDSPAAPYLNALARKGALLTNSYAVTHPSQPNYIALFAGTMEGVNGNVCPTPLTAP
                     NLSSTLKQAGQSFIGYSEDLPAVGAVDCIAGAYARKHNPWVNWQASPVNTVPAAQNRP
                     LTDFPTDLSTLPTVSIIVPNQQNDMHDGNDPDRIQRGDHWLQVRLERYVDWAQTHNSL
                     LIITWDEDNGKSDNHIPTILVGPMVRPGRYHEQTNHYGLLRTITDMYGAQPVGLSRQA
                     SPLTTIWAAPKLTP"
     gene            complement(29103..>31154)
                     /locus_tag="PK589_06055"
     CDS             complement(29103..>31154)
                     /locus_tag="PK589_06055"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNJ19196.1"
                     /translation="TELGRIATLMTTVPVEPTPLQRRLEQFGHVLLWLSLAIVVVVFG
                     LGLWRGEPLFEMFLTAVSLAVAAIPEGLPAIVTTTLALGVMRMVSRHALIRRLPAVET
                     LGAATVICTDKTGTLTKNEMTVTRLAVDDVVYDVTGEGYAPEGTIVDGDGRTGALRAL
                     LWSAVLCNGSSLRAAEGSWTVVGDPTEGALLVAGGKGGWRKEALERAHPLVGEIPFDS
                     ERKMMTMIRRSEGRAVAYVKGAPDILLERCDSYMTKSGEVAPLSDSMRRSMVSINQQF
                     AQQALRVVALAQRVLDPDPATFESKGIEQQLVFLGLAAMKDPLRPEAKQAVERCRSAG
                     ITTVMITGDHKETALAIGREAGFASGSTQALSGLELNGLTDAELSSRVRDVCVYARVS
                     AEHKLRIVRAWRAQGAVVAMTGDGVNDAPAVREADIGIAMGMTGTDVTKDASDMVVTD
                     DNFASIAAAVEEGRSIYENIRKAVHYLLSCNLSEVLVMLGSTLLGWPLPLLPIHILWI
                     NLVTDGFPALALAVDPKDPDVMKRPPRDPKAPLLDRQRFVTVCVQGAVMAAATLAVFG
                     IALVIMKDEVTFARTMTFTTLVLVQFLHAFTCRHDRFSLFQIGITTNRMLVAAVLISA
                     LLQAGILLSPWGQDIFRVVPLRLDDWWLVAACGMLPFVVMELWKAWSRVRNGAARTST
                     A"
     assembly_gap    31155..31254
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31255..31568)
                     /locus_tag="PK589_06060"
     CDS             complement(<31255..31568)
                     /locus_tag="PK589_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNJ19197.1"
                     /translation="MVEARQHSQTRWYARSLDDLGREFGVDQALGLDEDEAARRLTAH
                     GPNELPEAAPPSPWHMLGAQFSSLIIWVLIGAALVSGLLGEWVDACAILAIVLLNGLL
                     GFV"
     gene            31861..32313
                     /locus_tag="PK589_06065"
     CDS             31861..32313
                     /locus_tag="PK589_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19198.1"
                     /translation="MTRSSWSSSNPLPDNLRASLKDLRHGLLGLHKSLIVSEQLTYER
                     IYGRISSAGQLLQLVMNDPWFAWLHPLSHLVVRIDVVLEDHEDTTIETAQDLLLEARH
                     MLRPSEEGDGFERSYYEALQRTPDVVLAHASVKKLLSTAATAASAAAA"
     gene            complement(32332..32946)
                     /locus_tag="PK589_06070"
     CDS             complement(32332..32946)
                     /locus_tag="PK589_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19199.1"
                     /translation="MRERDVQAIGTRRGARVGWRTVFGIVAVAMMAQVSSAPAVVHVL
                     PDMTVTRDSGTVTGAKAVRADPTLQEVLAAFDRAERALEKQDLSSLMQFYAPGYNYHG
                     LHVEDVQRIWGEVFEHYRTLSSTHLFSEMKIVRAEDGLRVELTCTGGLYGADVQSGAR
                     ITLDSWFQEVHYLVRDRQGWRFLGNRGDAPRSAPFSSSPHHPLF"
     gene            33288..34277
                     /locus_tag="PK589_06075"
     CDS             33288..34277
                     /locus_tag="PK589_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19200.1"
                     /translation="MPSAMKQLLASALGYALMASGAGLAEEIPHQRSIALIDKTWAVS
                     LDMTGYRVQADGVKPDSRRYVLATNDAAAMQLSVTLEAVSGQATEQGCLAHLHRIAQT
                     AAAPSHAGPTRTEINQLPVFEYLLYETSAGWTDQVHLFACTNKDNVYTDIHLSHSGTT
                     TVDASQLRKVLESLSIVAAAQPGSLDYFRAGSAPYLQGQFKQAIPHYERAVALERTNP
                     SLDKALWRLLVHNLGVAYRRSGDLARAKTTIDYGLSQEPANPSFHYDLARTYAGMNER
                     DRAMQSLHDAFLHFRRSNDREPIPDPRQDVSFGRFMLDPAFRTLTESLMQPAI"
     gene            34326..34694
                     /locus_tag="PK589_06080"
     CDS             34326..34694
                     /locus_tag="PK589_06080"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ19201.1"
                     /translation="MSKRKNTRVEMNRPIDVQGPRGTSQGVVKDFSPGGCKIQQTAAK
                     VHCGMRLTLRISLPDRIEPIEIKPAVVTWTSKDAFGVEFLALSAETRSRVKLVYDLLL
                     DAQSTAETERVISLPGVSWK"
     gene            complement(34898..36415)
                     /locus_tag="PK589_06085"
     CDS             complement(34898..36415)
                     /locus_tag="PK589_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ19202.1"
                     /translation="MPGSPHSPNGLSYDPQVLLNSQPVIVTVIDPADHSVQFQNQTGL
                     GAFGDMAGSPCYEKIAGKTAPCEFCRMPEALSRDGVVSEQVEMPNGRHLLVHWAKAPT
                     KDGRVHVIETIIDLTKRKQDEQSLRQSQKMEALGRLAAGIAHDFNNLLMVVIGHAHRV
                     AQQLGSHPLHHEVEMIGQAGTRAAALTKKLLTFSRRQVLEQRDVPLNTLIRDMEDILR
                     RLIGEQIQTVIVLHPQAGHVLGDPVQMEQVLLNLALNARDAMAEGGILTIETGNTDVD
                     EQYVRTHPGAVPGPYVTLVVEDTGCGIDAETLAHIFEPFFTTKEFEKGTGLGLATVYG
                     IVKESRGYIDVTSQPGRGTRFTVMLPRVQSQTSEAEQPASSRPLPATCATILLVEDDE
                     GIRRLVAAVLRDQGYEVLAAADGVEALQMLQLRKGGCDLLITDVILPRMKASVLAQGA
                     RTMFPKIKVLYISGYAGDMLVSHGVDAQAAYLQKPFMPQAIIEKVAELIPPAPAQ"
     gene            complement(36565..39090)
                     /gene="mgtA"
                     /locus_tag="PK589_06090"
     CDS             complement(36565..39090)
                     /gene="mgtA"
                     /locus_tag="PK589_06090"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HNJ19203.1"
                     /translation="MLKQTPFWSLSADDVLRQLSATPNGLATAEVDARQATSASVRLK
                     PQRNSRPLRLLMAQFRSPIILILLFASGVSFFLAEHSDALIILTIILVSALLSFWQEY
                     SAARAVAGLLALVQITARAWRDGELRDIPADHIVPGDVIELSAGSSLPGDALVLEAKD
                     LFVDEATLTGETYPVEKSVGSLPAHAPLAKRTNSLFLGTHVVSGQARVVVVAVGTDTE
                     FGRIAHRMALRAPETEFERGVRRFGYLLLEVTLLLVLAIFAVNVYLERPALESFLFSM
                     ALAVGLTPQLLPAIISVNLSHGAKRMAQQNVVVKRLASIENFGSMNVLCSDKTGTLTE
                     GSMRLHAALDLDGQPSERVLFHAHMNAMFETGFPNPLDEALRQHRSFDLGSYRKLEEE
                     PYDFVRKRLSVLVATPQTHLLITKGAVESMLSVCLHAEQQNGSLMPMDQVRASIREQV
                     RGLSSEGYRTIGVAYRDLGAIDRVSKEHETGMTFLGLLVFVDPPKAGIVDTIALLRKL
                     GVSLKMVTGDQGLVAAHVGRQVGLANPRLLTGSDLRGMTDDALRARANEVDIFAEIEP
                     NQKERIIRALRGSGNVVGYLGDGINDAPALHAADVGLSVDGAVDVAKEAADLVLLEHD
                     LSVLVEGVREGRRTFANTLKYVFMATSANFGNMFSMAGASLFLPFLPLLPKQILLTNV
                     LTDIPEMTIATDHVDSELLDRPRRWDIPFIRRFMLTFGLVSSFFDYLTFGILLFLLHA
                     TTAQFRTGWFVESVLSASLIVLVIRTRRPCVTSRPSAPLLWSTLLIALVTILLPITPM
                     GSFLGFEPLPAVFWVALLGILLAYVAAAELAKTFFYRQVNNGR"
     gene            complement(39111..42239)
                     /locus_tag="PK589_06095"
     CDS             complement(39111..42239)
                     /locus_tag="PK589_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNJ19204.1"
                     /translation="MIAALLEFSLRQRILILGLACFCAAAGVFAFQSIPIDAYPDVTN
                     VQVQVLTEAAGLSPVEVERFITYPIELQMNGLPGLTEIRSISKFALSQLTVVFEDDVD
                     VYFARQLVLERIMAVRERLPAGMEPVLAPVTTGLGEIYQYYLEGGTKPDTNAALVEEA
                     LTNQRTIQDWVLRPLLKGVPGVIDVNSLGGFVKQYQVLVDPDRLRKYSLTLHDVFDAV
                     ERNNANAGGNVLERHAERAIVRGLGLIKSLADIERIVVKEAGGVPVFVRDVAEVRIGH
                     AVRHGAAVLNGEREVVAGTVLMLRGGNAREVVQAVKQRAESIERQGILPGGLTLVPFY
                     DRIELVTAAIHTVRDALIEGVVLVALVFFLFLGHVRSAIVVTVSLLVTPLITFVVMQR
                     VGLSANLMTLGGLAIGIGEIADGSLVVVENIYRHLSENRLQERSRLEVVLRATNEVGR
                     PILFGILIISVVFLPLMTLHGMEGKMFAPLAYTLVISLLASVIVTLTLSPVLASLILH
                     GDHPEETRLTRWMKAQYQPVLRWTLDHRAAVLLSSTAIVLASLSLLPSVGREFIPILE
                     EGALTPQIVRLPSVSLDESIAIEKQTQKVMLEFPEVRMSVSKIGRPDIAVGPEEPNES
                     DPIVALRPREAWITAQTQAKLVDAIRTRLAEIPGISVLMSQPIQERVDELISGIRTEC
                     AIKLFGDDLDLLYQHAERIADLMRTIDGVKDVKVEQVAGQPYLTIDINREKIARYGIN
                     VSDVQDIISTAVGGRPATQVYEGERRFQLVLRFPESSRNSIGAIGDIRVRSASGAPIP
                     LSELAAIEMREGPARISREQAKRRIYIGFNVVGRDIGGVVDEGRKRLAEQLRLPQGYT
                     VAWGGAFENMERANARLLVVVPITLGLVFFLLFWAFHSLRYAGLIILNLPFALIGGVV
                     SLWLSDQYLSVPASIGFIELFGLAVGNGIVLVSYINQLRNEGQQTEAAIISGCVLRLR
                     PVVMTMMTTLLGLLPLVLAQGIGAEVQRPLATVVIGGLFTSTALTLLVLPALYRTFAE
                     QDVTKEHAPEWV"
     gene            complement(42236..43513)
                     /locus_tag="PK589_06100"
     CDS             complement(42236..43513)
                     /locus_tag="PK589_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ19205.1"
                     /translation="MWLYTAGTCRTDNGHMTDRVAALTCEFRRLLPRPACSLHLAGRA
                     LLVVLLMGQVGCDRDPGVPPAAKPVSRAADGIVQLTAAEIGRAGIQVQRAAKEPFVLH
                     REFPATVQANENELAEVTTLIRGRVVDVLVDAGKDVKKGERLALLDSTDLGMAEGVYL
                     KATARQHEAQLAHERAANLHEHRAISLAELQRREAEMKTAQAEAREAGHRLRLLGVPD
                     QEIQRLERERTIRSDIAIRAPFAGRVIMRNLTRGEVVETSRNCFTIADLSDVWVIASV
                     PEKDVQFIHPNQTVHVIVAAYPHSLFSGRMTYISDVLDPTTRTMRIRVTVPNPDRALK
                     PEMFAMVRVDASPQPAVLTVPLSAIQQEGGGKMLFVRQGENRFAPRRVQLGDEQDGQV
                     IVLEGLREGEDVVVKGAFAIKSELDIHKIEPTQ"
     gene            complement(43659..44513)
                     /locus_tag="PK589_06105"
     CDS             complement(43659..44513)
                     /locus_tag="PK589_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ19206.1"
                     /translation="MKVLIATDGSKYGKWATEWVARMPFAEKPEVALLHVTDVEALRA
                     PFMFQPVVIGNEPFIQEEIKRIEARGKTAITEAKAQMASLKLKGKVQSERGPAGPTIL
                     DRAPRRDGIVAIGSRGLDALDRFMLGSVSTQVTLHAPCSVLIVKEEPRPVSRILFAAD
                     GSKASEKALRFLLTKLQPEAREGFEPIDVVVMHAMPFLKYPELKEAGARLVEQCANKL
                     IKAGYVVDEVVQLGKPADEILKVASKKKVDLIVTGAKGMGAVARFLLGSVSTKVVQHS
                     TCSVLVVR"
     gene            45279..45734
                     /locus_tag="PK589_06110"
     CDS             45279..45734
                     /locus_tag="PK589_06110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ19207.1"
                     /translation="MTQAGEPIRVELVDWVHAEQLIRPIRETVFIREQAVPEELEWDG
                     LDPQCVHVLAWNKQGDAIGTARMQAQGTIGRMAVLKDWRGRGAGRALLEALLECAVRQ
                     GLTRVTLSAQTHAIGFYERAGFHVIGELFMDAGIPHRRMVKELIRQESE"
     gene            45825..46640
                     /locus_tag="PK589_06115"
     CDS             45825..46640
                     /locus_tag="PK589_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19208.1"
                     /translation="MATTPDISRAWPPDDHYSLLQRCRSLYHELWFRLSERIRWSRQP
                     YHEKPIGCLAGLTSWQIARINELQVRYDLRFETHYGQHTALANYAYLDLLHQAWTDTN
                     RPVPQGGVVADVGCANFWYARTLHAFFRPATLTGVDVEGFRLYPTGYSRYDAARGYIA
                     GLSQTSFLVANYCEVETKADVITAWFPFVTPSPVLAWRLPLTVFSPERLFDRIARNLT
                     SQGLFFMVNQGSDEAAIAADYGRRAGLRLIDRWVHPRPLRTRPHPPVVTWWTT"
     gene            complement(46694..47017)
                     /locus_tag="PK589_06120"
     CDS             complement(46694..47017)
                     /locus_tag="PK589_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19209.1"
                     /translation="MMTRKILATLMFVWGIGLVSLAQAFQTAVVFPVSSVEHVDSRNQ
                     VLTFKTQDGQVRMFRVAQSVGMKRESLAKGDLVRIEVDLDDQIVNIVKLTQTSLSDRE
                     ALRRK"
     gene            complement(47253..49163)
                     /locus_tag="PK589_06125"
     CDS             complement(47253..49163)
                     /locus_tag="PK589_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNJ19210.1"
                     /translation="MPASLKHIDRDTPMGATLVGGGATFRCWAPHAKAVYVVGDFNHR
                     IRNDAALLTRDQQGHWRGFFPGVKDRQRYMFYVVGEGSEGLKRDPYARELESPFPAEC
                     LIRRTDFPWHESGYVTPPFHEFVIYQLHVGTFFTPNLPHKGGTFLDVARKLPYLADLG
                     VTALQLMPIQEFQTSFSLGYNGTDYFSPEMDFAVADAELPAYVAEVNKLLDAKGLRRY
                     HAADLHGEMNQLKALVDLAHLHGLAVLLDVVYNHAGGEFGDQSLYFFDRQDPAGGQWN
                     SLYFTDRGHAGGLVFDFSKPEVRDFLIQNAKFFLGEYRIDGFRYDQVSVIDHDGAPDG
                     WRFCQDLTSTVHAQRPKTLHHAEYWDVNPFIIAPPPVGAGFDTTLTDGLRIAIRDVIA
                     NASAPDERPLHMTQLAQSLWPQGFSESWRFVQGPENHDIVYNGREARIARLGDPDHPR
                     SWFARSRARVATGLSLTAPGIPMLFMGQEFLEDKQWSDDFVSHRDLLLYWAGLDQGDR
                     QMLDHLRFTRELLALRRLLPALRGRGFRATHMHDQNRVLAFHRWVEGEGQDVMVILHL
                     ANFTRVGYQIGLPGEGAWREVFNSDVYENWVNAGVAGNNGRVLANPQPLHGFGYSAGL
                     VLPANSILIFAR"
     gene            complement(49300..49836)
                     /locus_tag="PK589_06130"
     CDS             complement(49300..49836)
                     /locus_tag="PK589_06130"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNJ19211.1"
                     /translation="MVNMTMAPFALWLTGLPASGKSSIVARLLPKLASMDMTAEVLES
                     DAVRRILTPEASYSKEERDLFYRALGFMGARLLAHGVNVIIDATASRRAYREFARTLI
                     PDLLEVSVECPLHVCMERDKKGTYRRGLQGESSTVPGLQEPYEAPLSPDLVLNTTELL
                     PDAAADQIVRLIRAHRQS"
     gene            complement(49884..50972)
                     /locus_tag="PK589_06135"
     CDS             complement(49884..50972)
                     /locus_tag="PK589_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNJ19212.1"
                     /translation="MPVLKKSALDGYVKARFGSTAELVAYGPIGKETQGARYKQYGYG
                     APIRLTFRTNGNKTRQVVLGTMSPGPFGHEHPADRAQAMLMDYDCYSRLPRHITALDV
                     GAFTARGELMSVAAAKEFFLLTEWSEGDTYHKDLERLADATRPTPLDRKRTQALADYL
                     ATIHRVKRRDPQLYRRRLRELLGHGECIMGLTDSYPHRYGFITEELLQRIEVACNAWR
                     WRLRRQHARLSQVHGDFHPWNVLFRRGADFSVLDRSRGEWGEPADDVTSMSINYLFFS
                     LCRHGTLKGPLEVLFHSFWDRYLSRSHDQDVLTATGPFFAFRGLVLASPLWYPKLTLT
                     LRRKIFRFIENVLAVPRFDPADVNRYCD"
     gene            51257..51802
                     /locus_tag="PK589_06140"
     CDS             51257..51802
                     /locus_tag="PK589_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNJ19213.1"
                     /translation="MNLEVESRNIEMTPRWKTEIEERMAALQRGHDDIIHGRVTLTKN
                     RHHKKLDNVAEALVLVTVPSRQTITSRKEDKTFEEAIRSAFDAVAIELKKFREKRADK
                     VIRVEPLPQRRGVVSKVFPEQGYGFILMDGGGEVYFHKNAVKGMAFADLEDGVEVLFE
                     SEPGEKGLHATIVQPPHLLKL"
     gene            51813..54224
                     /locus_tag="PK589_06145"
     CDS             51813..54224
                     /locus_tag="PK589_06145"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ19214.1"
                     /translation="MLDNHRLPASLLAPTVDPARLGFADTSQVQPLEETIGQERAVEA
                     LEFGLQMKSPGFNIFVSGPVGTGKGTLVRQMVQRLAQTAPPPPDWCFVHNFQDASRPS
                     CLSFSAGQGASFKRDMSTFIEGLRRDIPTTFEGKKYLDAKAKIIEETEDKKKNLFHDL
                     TILCHQRGFVFEETAGGFALVPIKDDRPMTGKEMEELPESIQEELTARRQSLESDIRE
                     FHVRMHGLEREAEQALHHLDHQIVANVMKGAYDALLQTYQSSQLVVTYLQRVHDDIVH
                     QYRDFLPHTGPMLAIPGLEQSRRPDLTRFAVNLIVSREAAGGAPVIDESHPTYSNLIG
                     KIERRAHLGVMYTDFTEIRPGSMLQANGGYLILQALDLMRQPFSWEALKRVIKTGAVT
                     IEDPSEFFGFATAGLRPEPIPVSVKVILVGTAGIYHLLQAYEEDFSKLFKVKADFDVE
                     VPHDEHQERQYARFVSRLCREEGLPHFGADAVAEVIRQGFRFADRHDRLSLRFSLVSD
                     LIREAGYWAQKSGHVLVTRADVESALAHQRRRADLPEQWLQGEIAEGTLMVDLQGEVI
                     GQVNGLSVYELGDYCFGRPTRITARTYVGTKGVIDIQREVDLAGEIHSKGVMTLAGFL
                     AGKFAGMQPFALSATLTFEQTYSEVEGDSAAVAELAAVLSSLADLPVRQYLAVTGSVN
                     QLGEIQPIGGVNEKIEGFFESCKRRGLTGKQGVIIPARNIKHLALNREVVAAVEDGRF
                     AVYGVDTADQALELLIGVPAGERGAEGTYPPVSIYGRTAARLEEMAQIVATWGEAEED
                     NRVKD"
     gene            54258..54422
                     /locus_tag="PK589_06150"
     CDS             54258..54422
                     /locus_tag="PK589_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ19215.1"
                     /translation="MPMYDYTCLDCGKESLIVVTLKQHEKGEVQCPACGSTKLQQHFS
                     SFIAHTTKKS"
     gene            54440..54823
                     /locus_tag="PK589_06155"
     CDS             54440..54823
                     /locus_tag="PK589_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ19216.1"
                     /translation="MRTFTTLSIRHLILATAAGLMLAPVVDDANAQEFPPDAAKGKAV
                     YERHCLACHGAGGRGDGPDAAGLTVPPANFHRFKSFLKSDEELLRTIEHGVVFSPMHA
                     WQGQLTETERQDALAYIRLLVQQGK"
     gene            54874..55455
                     /locus_tag="PK589_06160"
     CDS             54874..55455
                     /locus_tag="PK589_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNJ19217.1"
                     /translation="MTNLTSHDALVVVDVQRDFLPGGALGIQDGDKIVPILAGYVAQF
                     HARGLPIFITRDWHPPDHCSFRDQGGPWPVHCIAGSPGSLPPPNFQPPVSAVTIYKAI
                     DQDKEAYSTLQGTPLDRHLRALKVRRLFIGGLATDYCVLNTVKDALGLGYDVCVLMDG
                     IKAVNLHPDDGRNAEEAMLHLGAVPVRLESLAA"
     gene            55452..56786
                     /locus_tag="PK589_06165"
     CDS             55452..56786
                     /locus_tag="PK589_06165"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNJ19218.1"
                     /translation="MTPSTTSLLTDLYELTMTQAYLAHNMTGEAVFEFFVRKLPTRRN
                     FFMAAGLEQVLSFLEQFHFSEGDLAWLEERGRFPPETLIAFRAMRFTGDVHAMPEGTV
                     CFPHEPILRITAPLPQAQLVETRVMNLLNFQTMVASKACRCILAASGKPLIDFGLRRS
                     HGAEAGLLAARASYLAGFAGTSNVLAGAQFAIPTYGTMAHSFVQAHRDETTAFLHIAQ
                     SQPHHVVLLIDTYDTEAAAERVVMLAPELKRQGIAIHGVRLDSGDLGAHARKVRHILD
                     RGGLEETTILASGNLDEERIEALVTSGAPIDRFAVGTAMTTSADAPYLDCAYKLQEYA
                     GRPCRKRSEGKATWPGRKQVYRRLGPDGRLAQDTITVEGDTLPGRPLLRQVMEGGRRL
                     APSPSILEIRDRTRLALSELPDTLGQLDQAEPYEVRISGALERLAQQVDRYT"
     gene            56852..58279
                     /locus_tag="PK589_06170"
     CDS             56852..58279
                     /locus_tag="PK589_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNJ19219.1"
                     /translation="MNQHSLSAAFVAGLVVTTLCSIPDGALAAAKSASKSALAVACES
                     QYQRTRPAPKVVDKVLQAHARWLEDRESADGRRANLCRTDLRQLRLTTASLERANLEG
                     AILKGANLRSANLVQAHLKGADLSHAILDDANLEGADLRKGLLAKAHLNRISADEAAF
                     YGANLQGAFFREALLERAHFEDADLQLADLSGASLLDGYFYGANLSKANLTDADLAGT
                     DLRRTNLRQATLRRANLQGALLDSATLDGTSLVEADLESAYLDDASLVQADLHEASLR
                     GADFRYARLTNANLQRANLENANIEGANLAKARLNSATMTMSVLYKANLTSANLHGAR
                     LHHAVLIDAHLSRADLQKADLTEVYAPKAHLQQARLAEANLELANLVSADLSEADMSH
                     SIMVQTNLQEANLRGTNLTAADLTGAQLNNADLQQANFRGANLSGALGLVQAQLDQAC
                     LDEATQIPTDLQRPKACPPPRQKRK"
     gene            58453..58983
                     /locus_tag="PK589_06175"
     CDS             58453..58983
                     /locus_tag="PK589_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19220.1"
                     /translation="MNLRRKTMFLLAGLAALAVTLLLIWCPPALCRVLNIYTFPLSNL
                     SDFRQYVADEEAFYSLHHTRAVRIDCAALRDEVVTVLKIGHLVLETNTPGDTQRERRP
                     GTIEAWHQGRLLHLTNTTLASKDEQQAWATSVRAVVTLQQQQDLAFLSRLFGAVVLHV
                     SHRQIPIVLFSDRPCL"
BASE COUNT        12652 a        18578 c        16115 g        11639 t
ORIGIN      
        1 gcccgggcac aactcatggc ggtcgtgact caacgcgcca atgagaaagc gtcggtgtac
       61 ggcattgaga ttcaagatgt gcggatcaaa cgagcagacc tccccgaaca aaacgagaaa
      121 gcggtctttt cacgcatgca ggcggagcgg gaacggcaag ccaagcaata tcgggcggaa
      181 ggcgcggaag aggcgcagaa gatcaagtcg gaagccgaaa aagaccggga aattatcctt
      241 gccgaagcct atcgcgaatc agaagaactt cgtggcggcg gcgatgccaa agcgttcaaa
      301 atctatgcgg acgcgtaccg ccaggacccc catttcttcg agttcacccg cacgatggaa
      361 gcctatcgca aaaccctcaa agacaagacc accatcctcg tcagtccaga ctctgagtac
      421 ttccggtttc tcaaacagcg ctaaccgcca tcacgacagc ccgacgatct cgccagactg
      481 gggatcgata tcgatgcgct cgcccgccgg ctttttgggc aggcccggca tcagttgaat
      541 gcccgagcat accgctgtga gaaagcccgc accggacagg attcgcaatt cttggatggg
      601 caccgtaaac ccggatggcc gccctttaag cgcggggtcg tgggaaagcg acaatggcgt
      661 cttcgccata cagataggaa gctgctcgaa ccctaatcgc gccgccaact ctatatcctt
      721 ttccgcctga gacgagtacg acaccgcggc tgcgccgtag atcgtggtgg caatggtctc
      781 gatcttcctc ttgatgggcc aagtggcatc ataaagaggt ctgaactgtg atccttgtgc
      841 agcgactttg acaacggccc gagccagttc ctccgcccct ttcccaccat cggcccaatg
      901 agtcgacaca gcggccgccg cagctcctgc agccagcgcg cgttcacaga cccactccaa
      961 ctccttaggt gaatcatctt tgaatgcatt cacggccacc accacaggca ctccgtgcgc
     1021 gcgcacattg gcaatatgct gttcgagatt ggcaaaccct ttttcgagcg cggccgggtt
     1081 cacttccgtc aaccctggcg gcagcggtgt tcctgctttt acaatcccgc ctcccccatg
     1141 cagcttgagg gcacgcaacg tcgccacaac cactccggca tcgggccgga gaccggatgt
     1201 acggcatttg atgttgaaga acttttccgc acctaaatca ctgccgaaac cggcttcggt
     1261 aatgacatag tccgcacatc gcaacgccaa ctgatccgag acgacggaac aattcccatg
     1321 agcgatgttg ccgaagggcc cggcatgaac aaacgcgggg gtgccctcca aggtctgcac
     1381 cagattcggt aagagggcat ccttgagtaa tacggacatt gaaccggcac aaccgaatgc
     1441 ctcggcggtg gtcatcgcac cgctctctgt caggccgacc agaattctac cgaggcgttg
     1501 gcgtagatca cgctgatccg tggccaatgc caacaccgcc atgatctccg atgcctcggt
     1561 gatgacaaac tgccctggtc gcggccctgc ttccgcattc aatacgatgt cgcgaagcgc
     1621 tcgatcactc acccccaggg tgcgcggcca tctgattcgt tgcggatcca gtttgagcgc
     1681 attgccctga aacagatggt tatccacgaa tgcggacaac agattatggc ttgcagagac
     1741 ggcatgcgca tccccggtga aatgcagatt gatatcctcc atcggccaga cctgggcgcg
     1801 ccctccaccg gtcccgccgc ctttgatacc aaagaccggc ccgagggaag gttgccgtaa
     1861 cgtcactgcc gcattatggc ccaatcggca gaggcccatg ctcaaaccta cggaggttgt
     1921 cgtcttacct tcgccaagcg gagtggggtt aatggccgtc acgaggatat accgcccgag
     1981 cggcctgctc ttcacgcgcg cagaaaagtt ggcagcgatc ttggctttgt accggccata
     2041 agggatgagg tcttccgaag gaatcccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnntaa tcaaggataa actttgtcgg atcaagcgcc tggccgttct tcttcaccat
     2221 gtagtggaga tgtggaccgg tcgaaaggcc ggtgctgcct acgagcgcga cgacatcgcc
     2281 gcgcttcacg cgctgcccct ctttcaccag ggacttggca agatgcccgt acacggtttc
     2341 aatgccatac ccgtgatcca gcttcaccat attgcccatt ttggaatcaa atgccacggt
     2401 gaccacacga ccaagggccg gagcctgtac gggggagttc gcctgggccc cgatatcgag
     2461 cccgtcgtgc caggctggct tctcagtgaa cggcgagact ctgggcccaa aacccgacgt
     2521 gacccaaccg cgaaccggcc agatcgacgg ggtggacgcc cattgggtcg agcgctgctc
     2581 ggcaacctgt gtcaggcttt ggagagcttc ttcctgctgc gtggcttccc gaacgagggc
     2641 ttctaagctt tcacgaacct gctgggtcgc ctcttcaatg ctctcggtcg agaaatccag
     2701 gtcggactgg ttattctcac gcagctccga tacctgctgc cgagattcca gtcctgagga
     2761 cgccggtcca ttccctggta cactgctctt tccatccggc aagggaccat cttcaccacc
     2821 tcgcccattg gcaaggtcgc cagcaggctt ggtcgtatca attcccagca tgacccgaag
     2881 ccgttgattg acctctccca tggccgacag tctcttcttc aaatcatcaa tggctgcaga
     2941 aaaggctgca gtttgctccc gagcacccat ggcctcagct cgaaaagcgg ataactgcca
     3001 gacttctccg gttcgaatca cataatggga tacaacgagc agatcggcca caatcaaaat
     3061 tgcagccagg atcaagagct tgcggacgaa tttccgtggg aaactaaacc ggagtggctt
     3121 ggaggatgat cctcgaaaaa ccacgacagt gtaggcatca cttgtttcag ccgtttgttg
     3181 gctcataggc tggtctcccc ttcacggatt cactttcaaa ccgcaaccac cgacttttgc
     3241 agtgtaccca tctcaagccc ggtggtcaac ccttagtttt caaaatttta catattagaa
     3301 tcagctactt agcagtttca ctcctgatcc agtccaggta cgaaaccgag cccgcgacga
     3361 tcggcaacgc gatgatttct gggaccgtat agctgtggtg ctgccgaatc acggcctcca
     3421 gctcagaata ccgctgcagg gtcgttttca tgatcatcag gcattcattc tcgacagtga
     3481 cctgattgtc ccatcgaaaa attgagcgaa tcccgctcac gatgttggcg caggcggcaa
     3541 gtctggattc gacgaccatt cgtcctagtt tctctgcttc ctcatttgac gaggcagtga
     3601 tgaataccac gacctcggaa ccttgcgcgt ccacgcctcc ccctgctaaa gcaatttgca
     3661 tgcccatacc tgtcgccaat cacagacatg ctgatataaa tcaaccactt acgggatatt
     3721 ttcccacatt ttccagcaca acgctcctac caatctgggc aggaattgca cagagaaaca
     3781 aaaaacggcc atcctgcgat gcaggatttt cccggcccca tactgttgaa ccaaccgtcc
     3841 ggcttatctg ataccgctgc tcttcttcgg actgaacgcg tcgaaccttg agagacactc
     3901 cccagcaagg cctcaccagg gttgacaccc cttttctgcc tccttatgat ccgaccattc
     3961 agtccagagg gaggtatatt gatgagcttt actattacac ccacagaact caagtcgagg
     4021 ttggataaag gtgacaaact tgtgctcgtt gatgttcgtg agccctggga atacgccatt
     4081 gccaagttgg aagggtcagt cctggtacca ctggccacgc tccaacagtc cctggggaaa
     4141 ctagatcgca actcagaaat tatcgcggtt tgccaccacg ggatgagaag cgcggatgca
     4201 acgggatttc tcttacagca agggtttggg aacgtaaaaa acttaatcgg aggtatcgac
     4261 gcctggtccg tccaggtaga ttcgtccgtg cctcggtact agcccgggag gctacccgag
     4321 tccatctcga aaaaattcca ccgttcgtcg caagccttct cgcaactcga ccttcggctc
     4381 ccatccgagt gtccgctgga gttttgcgca gtcaatgacg cttctcgctt gctcaccgcg
     4441 cttggccgga ccatgcactt ctttaaattc caccttggtc aggtccacga cgatccggaa
     4501 caggtcattg accgaagtct caatgccggt ccccacattg tacacaccct ctacctgagg
     4561 gcccatcgcc attaaatttg cttcgacgac gtcttccaca aacacaaaat cacgggtctg
     4621 gcgaccattc ccattcacga ctgcctgctc cccgcgcagc attttctgaa tgaaaatggc
     4681 cacgacgccc gcttcgcctt caggatcctg gcgaggtccg tacacattgg cataccgcaa
     4741 gctcaccacc tgtatgccgc tcatccggtg gtagtaggac aggtagtgct ccccgcacaa
     4801 tttgctgatc ccatagggag acagcggttg cgtgacatgc gtttccggtg cgggaaaggc
     4861 taattgctct ccatagatcg ccccgccgga tgacgaaaaa atgacctttc gcgcaccatg
     4921 cttggatgcc tgctccaaga cattcaacgt ccccagcaca ttgacctggg cgtcgaatag
     4981 cggatcttcg acggaccgcc gcacattcat ctgagccgcc aggtgaaaga ccacagaagg
     5041 ccgctcattg cggaaaaccc gctccagttt gggattttcg atgtccagct tatagaactg
     5101 cgccgccttg ggcacgtttt tgcgcttgcc cgttacgaga ttatccacca ccacgacatc
     5161 gtgcccctct tgaagcaacc gatccacaac gtgtgatccg ataaatccag cccctccggt
     5221 caccaagact ttcatacgtc tcctcgcctc tccttcatca gccgtaacag cagcatccgg
     5281 actgtatcgg aactcagcct acaccttctg tccgcttcct caaagtgttt tcgtcctgcc
     5341 cctgccagaa ggccattggc caggaccatc ctgcgacggc ctacggggcc ggattcaggc
     5401 cttccgctga aaccatgcga ggaattcgcg attgcctttg cggccctcga tcggagaatc
     5461 catgcggcct accaactgca agccgagttg caccgcacaa gacaccacct tttccaccgt
     5521 cgactctcgc aatgcgtcgt cccgcacaat ccctccccgg ccaaccagtc ccttccccac
     5581 ctcaaattgc ggcttgatca gcgtgataat ggaaccggac gcggcaagat gggaaacgac
     5641 cgcaggcaat acaagcgtga gggaaatgaa tgagacgtcg atcacgatca gatcgatcgg
     5701 ctccgcgatg gcacccggct cgaggtaccg cacgttggtc cgctccataa gaaccactcg
     5761 cggatcctgc cgaagacgcc actcaaattg accgtacccc acatcgacgg catacacacg
     5821 ggtggcgcct cgctgcagca gacaatcggt aaaccctccc gtcgaacatc ccacatcgaa
     5881 acagaccatt ccactcggat cgacctgaaa ctgattcagc gcgccggcca gcttttctcc
     5941 tccacgcccc acgtagggtg atccgggccc ggtcacttca accagcgcac ccggcattgt
     6001 cggtttcgcc gccttgtcga cgacgatgcc atccacacga accagccccg ccaggatcaa
     6061 tctggctgca tcctcacgac tggatacaag cccacggctg accagggcac gatccaggcg
     6121 ttcccgttct gggcgaggtt tctgagtcat acgtgtgaga ggaggttcga cgatgatggg
     6181 caggccacat cgtggcccgc gtagagccaa acgactactg tgggttctcc aactcatcct
     6241 caccgggaga aaacgcttga atgcgctttt tgccgtcttt ctcttgcacc agaatctcca
     6301 ccttccgttc ggcgtcctcc agcatcttca gacaggtctt cgacaaccga atgccttcct
     6361 cgaaaatctt cagtgaatcg tcgagaggaa ggtctccctt ttcaagttcg gcgacgatcg
     6421 tctccaatct cgccatcgca tattcgaatt ttacggcagc cacgacaact cccttttgct
     6481 catccgtcaa tcaaaacatt attgcctgcg ctcctggtcg ggtcaagata tcgacgaggg
     6541 catgaccttc tcgacctgac aaaccaattg tccttcggcg agtgtcacct caacggcctc
     6601 tccgactgtc acctcggagg cccggcgcaa gaccttccga ttagaaatcg tgtgaacgat
     6661 gctgaacccg cgctgtaaaa tggctaacgg actgagcgca tcgagcgcgg ccatctgcat
     6721 ggccaccgac tgccgcctgg acatgagccc gcggcgagcc tcctgctcta agcgcttgca
     6781 gagctgagga atgaccacca agaaccgctc aatccgattg cgcggtccct gcgcaagcaa
     6841 gaggtgtcgg cgctccacca gttcgcgctg cagcaccgtc agctgtgcgg tcaaggccct
     6901 cgttaatcca tcctgcaatt catcgagccg ctgagcctgc gcctgtatcc ggaaccgtgt
     6961 ctcgcccaac acgctgagcg atcgctcaaa ccggtgccgc tgcatctgca gcatggtatc
     7021 cacaaggcgg cgcaaacgcc gggccaattc atccaaccgt tccatgatct cggccaatac
     7081 gggtaccacc gcctcagccg ccgcagaggg cgtgggagca cggtggtccg cggcaaagtc
     7141 cgagagcgtc acatcgattt catgccccac agccgagacc accggaaccc tggatcgagc
     7201 aatggcgcga accaccgcct cttcgttgaa cgcccacaaa tcttccgacg cgccgccgcc
     7261 acgaccgacg atcagcacct ctacctgcgg catttcactc aagacgtcta tcgcctcggc
     7321 aatggacgcc ccggcgcctt cgccctgaac aggaaccggg gcaatgagaa tattgacggt
     7381 ggggcaacgg cggcgaagca cggctacaat atcccgaatc gctgcaccgg tcagcgaggt
     7441 gaccaccccg accgtcgaag gaaatgatgg caacctccgt ttgcgcgcct catcgaacaa
     7501 tccctcctga gacaaccgct ccttgagctg ctcgaatgcc agttgaaacg caccgacgcc
     7561 cttggggtcg agcgagtcga caatcaattg atactcgcca cgggcctcgt agacggagat
     7621 gcgcccgcgc gcaatgacag acaagccttc ctgcaaggca aaccgcagcc gcaaggctcc
     7681 ggaccgaaac aacacgccgc gaagttggct ctggctatct ttcagcgtga agtagaggtg
     7741 gccggaagac ggcgcacgaa gattcgagat ttcgccctcg atccagacat cctgaaattg
     7801 ctcttccaga gactcccgga tgagccgcgt cacatcggac acggacagca gcaatggcag
     7861 cgcaagattg tgcccggtca tggctcaccg agcggaaggg aataagtcag cggacagatc
     7921 aatccatcac tcggatacgt tcgatcgaca ccgctttgcc gagggtggca tccaggtcaa
     7981 tgaggacggc gcagaacacc gtcgggcccg acgccacctc aaaccggcgc ggcatccccg
     8041 tgagaaactt ttcaatcgcg agctcttttt tgataccgat caccgaatgt aacggaccgg
     8101 tcatgcctat gtcggtgatg taggccgttc ccttgggaag aatctgctcg tccgccgtct
     8161 gcacgtgcgt atgagttccc gccaccaccg tcaccagccc atcaaggtag tgtcccatcg
     8221 ccatcttctc agacgaggct tcggcatgca tatcaaccac gatggccgac acctgcgact
     8281 tgagtcgctc gacttcccgt ttggcaactt gaaacgggca atcgatggtc ggcatgaacg
     8341 cccgtcccat gagatggaga acccccagcg actccccgcc cggcgtcgtg aagatgtaac
     8401 tacctcgccc gggcaccccc gcgggatagt tcgcaggccg cagaagacgc ggttcccgag
     8461 gaaacacgtc taaaatttct ttcttatccc aggcgtgatt ccctgtggtg atgactgaaa
     8521 ccccgaggtc aaagagatca tccaccagat caggcgtaat gccgaatcca ccggccacgt
     8581 tctcgccgtt gccgacgacc acatcgatgg aatgattggc aatgagcttc ggtaggaggc
     8641 gagccacgga ccgtcgtccg ggctcgccca tgatatctcc gatcatcagg actttcatac
     8701 gctgtcgctc acttcgcaaa atcgacgaac cggctctccc ggatcaccgt gaccttgatt
     8761 tgccccgggt acgtcagttc ctgttcgatt ttcttggcca gatcccggga gagctggaaa
     8821 cattccggat ccgagagatc ttcttgcttg acgatcacgc gaatttcccg tccggcctga
     8881 atcgcatagg ctttctgtac gcccttgtga acggttgcca gcgactctag cttttctaac
     8941 cgcttcacgt aggactccaa cgcttcacga cgagcgccgg gacgcgccgc cgagagcgct
     9001 tccgccgccg cgaccagcac ggtctcgggg caaataggct ccacctgctc atggtgcgcc
     9061 gcaatggcat tgaccacctt ggcgttttcg ccgtatttct tagcgatctc tgcgccgagc
     9121 atggcatgcg gcccctcttc ctcgtgactc accgctttgc cgatatcgtg caacagcgcc
     9181 cctcgcttag cgagtttcac gtcgagtttc aactcggagg ccatgatccc gcaaatgtag
     9241 gccgcctccc gcgcatggta caaattgttc tgtccgtagc tcgttcgata tttgagccgt
     9301 cccagcacct tgaccaactc gggatggaaa tccgacaagc cgacttcgaa gatgaccttt
     9361 tccgcctctt cgatcatcag cttctcgata tcgaccttta ctttttcgac gatttcctca
     9421 atgcgggtcg gatgaatccg gccatcgtgc atgagccgct cgagcgacac cttggcaatt
     9481 tcgcggcgaa gggggtcgaa gcccgagata atcacggctt caggcgtttc atcgataatg
     9541 agatcgatac ccgtggccgc ctcgatagcg cgaatgtttc gcccttcacg gccgataatc
     9601 cggcccttca tggcatcatt ggcgatcggc accaccgaaa tcgtggcctc gttcacataa
     9661 tcacgggtca cgcgctgaat tgaagaggcg atgatctccc gcgcttcacg atccgcgttt
     9721 tctttcgctt cctcgagcaa ccgcttggct aaaccagcag cctccaagcg agcctgacta
     9781 tccatctcct gaatcaattg ccgcttggct tcttcggcag tgagtccggc cacccgctcc
     9841 aacgcctcgc gatgctgctt gagcgcctgt gcacaggctg catctttctg cgagagcacc
     9901 tcttcccgct tcaggagatc ctgctcccgc ttgagcacat cctgatcacg cttctcaagc
     9961 aggctgactt tcctgtccag cccctcttcg cgctgagaca cccgccgctc cacattcgag
    10021 acctcggcaa gcttcgcttt ttgttccttt tccagctcga ttcgtgcttg aaacacgagg
    10081 tccttggctt caagcttggc ctctttcacg aggttttcgg cttcacgctg ggccgactgc
    10141 acgatctgcg tggactggtc ctcagcctcg gcccgtcgtg taatggcaga ccggcggcgc
    10201 aacacttcat acagaccggc gcccaagacc gctccaacta acccgataaa aatgtacgct
    10261 acaacgctga gagaaatggg aaccacctcc cttacggcag tgggacaggc acactgtcac
    10321 cctgcaaata atcgcagcca gtggtaaagg gagcaggaag tgaaggaata gctggacagg
    10381 aaggaacctg actacttgag ccagtcgaac acgaagtgcg gaatcgccgt cgctagtcca
    10441 agtagaggcg tggtcggcct cgacctagat cgtttcttac gtgagctcgt gcacgaacag
    10501 agccgagccg ccttgttcga actgttgcat attcaagcca cggggaatgc ccccagatcc
    10561 tggtgagcct ctggttctcc aacatcggcg ttggttgatg acacacctgt cgcacgggca
    10621 catagggccg agccccctga ctcatccatg caatcacgac cacactgaac agcagcacaa
    10681 aaaccagcga ttccatatgt ttagagctca aataccgtcg tcagattttc cgtccttatt
    10741 cgaagcgtcc tttcctctcc tgctcttgtt gaccctcagg tcaacacaac gaagaaacga
    10801 taacaaagga gtgcagtgaa agcaaggtga aactcaacgc gacaagatgg acggcatctg
    10861 ttgatcgatc gactccatga gggaggctac gcggcggtcg gcatcggctt catcctgccg
    10921 aaacctccgc tccgattcca ttaactcatg ggcaagatta aatgcggcaa gcacggccaa
    10981 tttggccggg gtggccgacc gcataccggc cgccacctgt ttcatttgtt tatcaacaat
    11041 ctcggcgagt tgcttcacgt aggattcgtc ggcctcacca ttcacggtat accgctggcc
    11101 atagatctcc acgtcaatgg ttttagtcaa acgccacctc cttgggttca tccaggcact
    11161 ccagcaaatc aatttcgccg agcacctttt cgatccggga ccggatatcg agacgttcct
    11221 gctcccagcg ccggttttca tcatcgcgca ccgcgacccg ctcacgcgcc aggcgaagct
    11281 cgtcctccaa cgacgtgttc ttccgcttga gatcctgcac cagcttcacg agatcacgaa
    11341 tccgaatttc cagcgcatcg agacgatcta aagtcatggt atttcctcat tcaaagtgat
    11401 ggtcacacac gatggttaat gggggcgaaa tatagaaagt tcatgccccc ttgtcaagaa
    11461 aaccggtctc gtcatgacgc gccgtggagc cggcgatatt ctccgtaact gcgcaagacc
    11521 cgcttcacat acaatcgcgt ttcctggtac ggaatgagct ccacgaactc gtcttgatca
    11581 cgtccccgat gtactgcgat ccaattgttc acggcaatcg gcccggcatt gtaggccgcg
    11641 accgcatggg caagattgcc gctgtactgc tcgatcaata gccccagata ccgcactccg
    11701 aggcggatat tcgtttcctg gtcaaacaag tcttcccggc cgaccgtcgg gaatccgtac
    11761 cgttgcgcga ccgcattggc tgtcaccggc atgagctgca tcaagccaac cgcccccacc
    11821 actgagaccg ccttttcatc gtattggctt tcctcccgga tgatagcggc tgcgagatag
    11881 ggatcgacgc cggtcacgcc ctgggcggcg atcgttggaa cgaggccagt gggataggcg
    11941 acagtccata gggccggtgc cgtcggcaat ccacttcgct ctaacttttc ccgaaaatgc
    12001 actttcgcta cgcgtaaggc cggatgatac gcccccactt cactgagcat cgttgaaaag
    12061 gctagcagca catcctgatc gcgactgtac tgctcggtca gcgctcccag ctcccgcgcc
    12121 gcctcttggg caaaccccag gattttgagc tcgattcccc gctgatacac cgcgtgcttt
    12181 tcgatttcgg gacgccgatt ttccggcaac cgttcgatct cttccccggc cggccgttca
    12241 accgatccca ctgccggctg gttcgccggt atcgacgttc gtcgcgcagc gagctggcaa
    12301 taataactaa aggcatggcg ctgacagacc cgcgcgtatt ggtctccgac gttcgcgttc
    12361 tttccatgct cctcggccct cgctgcccaa tacatcgcct gaggttcaaa gccattgacg
    12421 tgcaactcca cgacggcacg gaaggtctcc gcggcatcct tataccgggc ggtccgatat
    12481 tgcgcccacc cggcccgcca gagcccttca gcccgttggc tggctgaatc ccccagcttc
    12541 gcgacctgcc ggaacatgcc gatcgcctca tcgaacttgc cctgatcctc caaccacacg
    12601 ccggcgaaga gatgcaccat cgcccgttgg tcaccggcta gtggaccctg ggctgccgat
    12661 cgtgccaatt cgatgagttt gtcgccaagg ctttgtcgca aatacacccg ggccaaccat
    12721 accgtggctt ccgctgattc ctgcactcgg tcggccacga ggccgcggaa tgtttcacgg
    12781 gcctggtcat attgtttgag ccgaacatag gcgaccccca acttgaggcg agcctcatgc
    12841 cggcgggcat gcccgggagc caacgagaga aaccgccgca actcctccac ggcctcggct
    12901 tgcattgaga gtccaagaaa cgcctgcgct cgaatcaaat attcctcggc agtcggcagc
    12961 caagactctc ctcccagacc gccctccaat cgcgttttcg cgtcgcgggc ttccggtgat
    13021 tggggataac ggagccagag ctgcttcagc gccgtgcggg cctccggcag ccggttctcg
    13081 cgaagatgac agtccgcctg gtgggataag gccatcggag ccgcctgatc cttatccgct
    13141 agcgccacgg cacgctccaa ccaatcaacg gcgcggaaac atacattcgc gctgtaccag
    13201 gcctctcctg ttcgataggc cgcctttgcg atcaggtttg aatccggcac aatcttggga
    13261 atcgtttcca gcagttcggc ggcttggatc ggttcgttct gtttcgacaa cgactccgca
    13321 atccaaagac gaaaataatc gtcgatcacg ggcaattccg gctgagcggc ccggagaagt
    13381 ttggtggcct cagccggttc tcgctcgatg agcaggacgc ctaacacgac ccgagctcgt
    13441 ggagcccatg gagtcgacgg aaacaactcc atcacggatc gcagctggtc gattttgagc
    13501 gccgtcgcct gctcccgctg ggcgggggaa ccggttcgat cattgacggc gagggcggaa
    13561 cgaaagcaat cttcagcgga gacacagaga ggaggaagca ccggcaacgg acgaggggac
    13621 tccgctatcg ccgtcaagaa tacactcgac agaaaaagac acacggcgaa tgccgctata
    13681 ctggtccgcc aaggaagtcc gcgcacaatg cccctgagca gaaggtgaac cgacatcatt
    13741 atagcgtaac gccatagaaa cgcgaccagg ttcgccttgc ttgtcacaag ttttttccct
    13801 gtgctaggct ccgtcatgct ctcacacact gcgacacgtc ataacctgct ggcgttgacc
    13861 gaatcggaca tggccgcatt cgtggcctcg ctgggctggc cggcctatcg cgcctcccaa
    13921 atccttcgct ggctctatca ggaacgggtc cgcacctttg ccgacatgag caacctctcg
    13981 cagaaggacc gtgagcatct cacctccacc tgcagtatcg agcgagccac ggatgtccac
    14041 atctttgcct cgcaggacgg cacgaagaaa tttgtgctca cgctgacgga cggcaagcag
    14101 attgaatgcg tcctcattcc cgacgaagac cgcctcacac tgtgcctgtc cacgcaggtc
    14161 ggctgcacgc tcgattgcgg attctgcctg acgggcacaa tggggctcac gcgcaatctc
    14221 cgcgcccatg aaatcctcga ccaggtcctg acagcccagg accatttgga agccgaccaa
    14281 cgcctgacga acctggtgtt catgggaatg ggcgagccgc tggccaacct cgacgccgta
    14341 gccgacgcgg tcacacgcct gacgaatcaa acatggggac tcggattgtc gggtcaccgg
    14401 atcaccattt ccactgcggg tctcgcgtcg cgaatcaaag aggtggcccc cttgaaggtc
    14461 aacctggcga tttcattaaa cgccaccacc gatgagttgc gccaccagat catgccggcc
    14521 gcgaaccggc tccattcgct ccaagccctg cttgccgcct gccgcgacta tcccctggct
    14581 gaacgcgatc gccttacctt tgaatacgtc ctgctggcgg acgtgaacga tcggcctgag
    14641 gatgcgaccc gattgatcaa actgctgcgc ggccttcgct gcaaggtcaa cttgatcgcc
    14701 ttcaatccct ttcccggcag tccctatcga cgcccgtcgg atgccgctat tgaccagttt
    14761 cagaacatcc tgcgccgggg ccacgtggat gcgtacctgc ggcgcagtcg cggtcgcgat
    14821 gtcctgggcg cctgcggcca gttgggtcga atcgagatgc cgcaagtccc ggttaccttg
    14881 acacagattc agacccgttg ttagcatgat cttcgtacat gcctaagctg agcacatccc
    14941 acagatcatc cctgcttcgc cattcagtat ttgcttgttc gcttctaagc ctcctgctgt
    15001 tcaccagcca gagctccacc gcccatgccg tcgagccgaa agagttcacg ctgtccaacg
    15061 acatgaaggt catcctcgtg gaggtcccga aggctcccgt ggcgactgtg caggtctggt
    15121 acaaggttgg ttcccgcaat gaggtgatgg gccgcgctgg gctctcgcac atgctggaac
    15181 atatgatgtt caaaggcacc gcgaaatatc ccaaggggac gttttcccgc ctcatccgca
    15241 aaaacggcgg aatggacaac gccttcacca gtcaggactt taccgcgtac tttgaaaatc
    15301 tggccgccga tcgcgtgacc ctggcccttg agcttgaagc agaccggatg cagggactga
    15361 ttcttgatgc gaacgaattc aaaaccgagc gggaagtcgt caaggaagaa cgtcggcttc
    15421 gcaatgagga tgatccccag ggcgcgctgg tcgaagcgct gttcgcacaa gccttcatga
    15481 gccatccgta tcattggccg gtgatcgggt ggttttccga cctcgatgcc atgagcctgg
    15541 acgatcttca gcgccattat gatacgtatt attcgccgaa caacgcgacc ctgctcgtcg
    15601 tcggcgacat caaggctgag acgctccttc ccgtcattgc cagactcttt gaaccgattc
    15661 ccaaaggtcc ttcgcctaaa gccctgactg tcaccgaagg tgcgcagcac ggcgaacgac
    15721 ggtttctgtt gaaacgcgaa gcccaagtac cctttgtcat gatggggtac cgggtgccta
    15781 actacacgag cgacgattcc tatgcgttga acgtgctcga gtcgattttg tcccacggaa
    15841 agagcgcgcg gctgtaccaa agtctcgtct atgaacagaa gaccgccttg gctgtgggcg
    15901 cggattacgg cttgatgcag gccgatcctg gcttatttta tttttatgcg gtcgtgaaac
    15961 caggcgagaa agtcgaagct gtggaggacg cggtactgaa agagattcag cggctgcaaa
    16021 cggagccacc cagtgagctc gaactccaac gcgccaaaaa ccaaatcgaa gccgcccaca
    16081 ttttcgaaca ggattccaac ttccgccagg ccatgctgct gggcgaagca gaaacgatcg
    16141 gggctggatg gcgtaaagtc agtcagttcg tcgaacgtac ccgtgccgtc acgccacagg
    16201 acgtccagcg tgttgcctcg caatatctgg ccgccgatat gcgcaccacc gggacattga
    16261 ttccgctccc gccgcaaact caatcggcgt cgccggccca accgcaccag tgagcacata
    16321 gatgatgcga cgacaggttc cgcattcacc ccgacgccac cggtcaccag gccggttctt
    16381 tctcggactc ctcctgctgt ttgcctctct gccgagcaca caagcacagg ccgccgacat
    16441 cgttccgagc cgctccgtga cggcaaacgg catgaccgtc ctgtttcttg aacaacactt
    16501 tcttcccacg gtggaaattc atgccttggt gaaggtgggg tcggctcaag atccgccgga
    16561 taaagcgggc ctggcaaatc tcacagccag cctcctggac gaaggcactc aaaccagaac
    16621 atcccggcag attgctgagc agatcgattt cgtgggaggc tctcttgaag cccatgcggc
    16681 ggaagatttc accaccgcat ctgcgcgcgt gctgaaaaaa gacgcggacc ttgggtttgc
    16741 cctcctcgcc gatgtgttgc aacatcccgc tttccacaaa caggaattcg aacgggttcg
    16801 cacccagatc ctcggtgaaa ttctcagcga tgacgacgat cccggcaatg tggccatgaa
    16861 ggcctttcat caattgattt tccatggcca tccctatagc tggccggcgc atggcgccga
    16921 ggacacgctg aacaaaatca ccgtggccga tgtccagcag ttccacgcgc gggaatattt
    16981 accgaatcag acgattctgg tcattgtcgg ggacctgacg caggaacaag cagccacatt
    17041 ggtacaaacc cactttggct cgtggaagaa gggcgcgcca tcgccctacc agcttaaaaa
    17101 gccgacgccg atcgaccgga aaatggtgca gttgattgaa aaagatctca cgcaatcgac
    17161 cattgtgctg gggcataccg gcatcagccg gacgaacccg gactactacg ccgtcacggt
    17221 catgaactac attttgggcg ccgggggatt ctcttcacgg ctcatggact ccattcgaga
    17281 caagcaggga ctggcgtacg gcatcatgag ccaattcgat tcccgcctgc tgccgggcgc
    17341 gttctacatc agcctccaga ccagaaccga ggtcacgaat caagccatca ccggggtcct
    17401 gactgaaatc aaagggatac gcgacgcccc ggtcacggat caggaaatca atgaagcgaa
    17461 gtccttcatc gtcggcagct tccctctgcg ggtcgattcc agcgccaagc tggccaacgt
    17521 cctcgcacag gtggagttct ataatctggg attggattat tttacgaact atcccaaagc
    17581 catcgaaaag gtcacgaagg acgatgttct tcgcgtggcg aaacagtatc tcgaccctca
    17641 acattacgcc ttggtcgtcg tcggttcgat tgcgaaagcc aaagtgaaac aatagctcct
    17701 tccttgcccg actcgacgac cgcgcaacac gtcggactcc ccatggtctc cagcacacca
    17761 ctgatcgaaa agatttcacg ggcacggcac ctccttcgag agatgggctc ggtgatcgtg
    17821 gccttttccg gaggcatcga cagtacgttg gtcctcaaac tggcgcatga tgaattggga
    17881 atgcaggcca ccggagtcac cgccgtctca cccacgcttc ccgcatcgga actcgcgctg
    17941 actcagcaac tcgcgacgga aatcggagcg cgccatcggg tggtcgagac ggatcaactg
    18001 gaaatcccgc agttcgtccg caatgatgcc acccgctgct accactgcaa gaccgatctc
    18061 tattccctct tgggtaaatt gagaagcgaa gatgcgacgg cgtgtatcgt ggatggcacc
    18121 aacgttgacg acttaggaga cgatcgcccc ggtctgaagg ccgcccggga acatggggtg
    18181 cgcagcccat tattggaagc cggattcaca aaagccgaca ttcgcgaagc tgcccgcgaa
    18241 ctgcacctct ccaattggga caagcctgcg gctgcctgcc tctcctcacg cgtcccgaga
    18301 ggcatcacga tcactcgaag cacgctctcg cgcgtggagc aggcggaagc ggctttgatc
    18361 cagggaggtt ttcgccactg ccgggtacgc gactacgggg acctggcgag gattgagttg
    18421 gccgtagagg agctgccgaa attgttggaa gcggggtgcc gcgaacagct cgtcgagagt
    18481 ctgaaacaga taggctatcg cttcgtgacg ttggacctgg aaggatatcg ccagggaggt
    18541 gtaagtctca cccccccggc gtcagaaact tagttccggc tctggtaggt cttcgcaagt
    18601 gcgtccaggg cttcatctgc cagacgacgg tgatccccgt cggtcaatgc tcgcccgacc
    18661 actttctccg caaccatcat cgctaaatcc gtcgtttgcg agcggatgtc ctgaatggcc
    18721 ttacgacgtt cggtctcgat ctcacgagtc gcgtcgccct tgatccgctc tgcatccgcc
    18781 gtcatgcgct gttcgttctc ttccatcaac cgtgccgcgc gctctttggc ctgagccaag
    18841 agtgcctccg cgtctttggc tgcggtggcc agcttggctt catattcctt gagcttgcgc
    18901 tcggcctccg aacgatggcg ttcggcttga tcgagactgt ccttgatctt tttctcacgc
    18961 tcctccagca tgcccaacaa gctcgggaac gcgtacttgt agagcaagaa gaagagaatc
    19021 ccgaaggaga gaatctccca gaagatcaac gacgaaaaga aatgtgattc aaattgaggc
    19081 atagtctgtt acccaatgag ttgagggttc tgagccgggc ctgtccccag catcagggct
    19141 cgaccgtcgg tgcgcttact tgcgcaagcc catgatgatg aacgcaatga ccagcccgta
    19201 caaggcgata gcttcgacca acgcgaaacc gatccacatg tacttcccga cgcgaccttc
    19261 ggcttccggc tggcgggcca cggcctcaat catttttccg aagatgtacc cgattcccac
    19321 gccggctccg gcaaaacctg ccgccgccaa ccccatgccg accaacgctg ctgctgctgc
    19381 atccattgtg tgtcctctcc tccgtttata gaccactcga cctagtgtgc gtgctcgcta
    19441 tgaccgtgca aggtcacggc atcccctaaa tagacacagc tcagcacggt gaagatatag
    19501 gcttgaataa acgcaatgcc gacttcgaga ccattcatcg caatcgtgaa ggcaaacggc
    19561 aaccatccga tcagcaatcc accactgata gccaatccga acagcacgcc taaaatcacg
    19621 tggccggccg tcatatttgc gaacaaccgt accgccagtg aaatgggccg cgccagttga
    19681 ctgatcaact caatcggaat catgagcggc agcaaccaac ccggtgtgcc aggcggcaca
    19741 agaataccca ggaatttcat tccatgaagc gaaaatccca gaaccagact cagtccatag
    19801 atccccaccg caaataccgc ggtgacgatg atctggctcg tgacggtgta ggaacccgga
    19861 atcaagccga gcagattcgc gaagaggata aagagaaaca gcgtggcgat cagggggaaa
    19921 tatttcattc ccgcttcacc catggtgtcc aaaataattc cgcgaatgaa ctcgacgatc
    19981 aattcggcca tgttctgcaa cttgccgggc actaacttac gtgcggcggc ggcagagatc
    20041 atgagaaagg caaccagccc gaccaccacc cacatgagaa tcaccgcttt gttgatggag
    20101 atatcgacgc cggcgggcac gagcggaatc aggtcgtgca attcaaacgc atgtaacgga
    20161 ctttcttcca cgatgatcct ttgtgagtca gacgattcag gttaactctg ccatcgttga
    20221 gccgatcgat aggcattgcg gataccggca gcggcaccca ggcccaatcc tgcgaccaac
    20281 ccccagggat tggaatccag cagatacgta tcgaccaccc agcccaatcc gcccccgaca
    20341 atcaacgcag cgagcagttc cgtcccgatc cggacagctt gcccgagccc cgcatataac
    20401 ggatcctgag aggggggcat cacgtgctca cgcgcggcac tcctcagcac agtcggagag
    20461 agtgcaccca tcggcgggcg cgccattcaa gcaaattcgc tggggacttg tcaagaagtt
    20521 ggcctgctat gacccggcct cacgatgcgg tatagtcttt ttctcgggta cgacaccgtg
    20581 actgacaccc tcatgactgc tgtatcgcac caggcatttc tcaacggccc tccacaacca
    20641 ctggtcgtgg ttcgaccgct gccgaacgcg gacgccttcg aactctatca gcgcctgggt
    20701 attacggacg gcccgtcctt tctcctggaa agcgggaatg gcaccaacgc taccgcgcgc
    20761 tattcgttct tcggcaacca tccgtacctt acattgaccg gccgggcgca ggactattcc
    20821 atgaaggccg acgggcacat cacaaccgcc catggctacg cgttcgatgc gttccggcag
    20881 gccttcgccg attccacgat ccctcagccg gatggtctgc cgccgtttta cggtggggcc
    20941 gtcgggtacc tcagttatga tctcgtccga tcgtttgaat cactgcccac cttggcttct
    21001 gacgacctcg ggctgccaga cctgcacatg gccttcttcg ataccgtggc cgcagtcgat
    21061 caccacaccg atcagctgta tctaatgtac tgccctccgt tgtcacgatt tcgggctgaa
    21121 ccgcgggaaa aactgctccg ggaaggcagt gaccgattgg cggagttgga agctcgcctg
    21181 acgagcatat cccggcctca ccctgccccg accggcccca tacaaaccag ctttgtcccc
    21241 agtcagtcgc gtgacgccta cacggctcgt gtccggcgct gccaggacta cattgcggcc
    21301 ggcgacattt atcaggccaa cctttctcat cgcttcaccc tcaatctgga cgctagctgg
    21361 tcggctgcgg atcgcgaccg atacgccggc attctctaca gccgtctccg ccgcgtgaac
    21421 ccctctccct ttgcgggact cgtgcgattt cccgagctca ctctcgtcag cagttccccc
    21481 gaacgactcg tgcgattaac cgcctcgcag gccagcacca gacccatcgc cgggaccagg
    21541 cccaggggaa acggcgagtc acacgatgtg accatgcgtg ccgaactctt ggccagcccc
    21601 aaggaacgag cggaacatgt catgttggtg gaccttgagc ggaacgacct gggaaaagtc
    21661 tgccaatacg gatcggtccg ggtggacgag tttatgacca tcgagcaata ctcgcatgtc
    21721 agccatctgg tctcggaggt ggtcggaacc ctgcgaccgg gcacctcgcc tttcgacctc
    21781 gtccgagcgg tgtttccggg cggcaccatc accggcgtac ccaaactacg atgcatggaa
    21841 atcatcgacg aactggaacc ggtgcggcgc ggcctctaca cgggagcgct gggctatttc
    21901 agttggagcg gggatttgga tctgaacatt ctcattcgaa cgttggttct cacgaaagac
    21961 cgcggctacc tgcaggtagg ggccggcatt gtggccgatt cggatcccga tcgagaatat
    22021 gacgaaacgc tcgccaaggc gggcgctttt cttcacatcc tggaggctga ccgttgatgt
    22081 gggtgtttct gaatgatcga ttcgtgccga aagaacaagc cgtcgtttcg gtgtttgatc
    22141 acggatttct ctatggtgat ggcgtctacg aaacgcttcg ctcctacggc tctcgcattt
    22201 tcatgcggga ccaacacctg gcccggcttc ggcgctcggc agaggccatc gggctgacga
    22261 ttccgattcc ggacgcgcaa tggccggacc tgctccatga ggccatgcgc cgcaatgagg
    22321 tgggcaatga tcgggtcgat gcctatctcc gcattacgat ttcacgcggc gaaggagaga
    22381 tcgggctgga ccccgcactc tgtccccgtc cgacagttgt cattttgacg aaggtgctcc
    22441 atccctcccc gccggcactg tctcgcgacg gagtcacgct caccgttgcc aacacgaggc
    22501 gaaatctgcc cgaagccctg tcaccgcaca tcaaatccac gaatttcctg aacaacattc
    22561 tggcgaaacg cgaatcgatc gcggccggga cttttgacag tctatttcta aactggcgcg
    22621 aagaactgac cgaatgcacg atcagtaatc tcttttttat ctcgaacgga accgtgcata
    22681 cgccatccct ggactgcggt atcttggacg gtatcacgcg agcgatcgtc atgactctcg
    22741 cggaggagga gggcctgcca gttcaggaag gccattacca cctggccgat ctccgccagg
    22801 ctgatgaatg ttttctgacc aataccagca tggaagtcat gccggtttca cgcgtcgacg
    22861 cctggccgat cggtgacggt cgtcccggcc ccctgactcg ccgcctgcag gcgcgtttcg
    22921 ctgccagccg aacccggttt cttgcaccag ccccttagac gcacacaccg ctattacccg
    22981 tgatatcagt gccttatagg gaattccagg ggtaggaagg agtaagggtt tttgacaggt
    23041 tgggggtcga ctgctacggt taaatcatcg gcaaagcaca ccgtacaggc acaggcgcct
    23101 caaccccgat ggcccgattc aaacgcacca cgcccctcct tctgctgtac gcaacactct
    23161 tgctgacaca cgcacccgtc caggctgagg tctaccaata tatcgacgca aacggcacca
    23221 tttccctgac caatgtaccg aatgatccgc gatataagcg ggtgatgtcc gaactgcctc
    23281 gctcgcgcag cgttatttcc gaccgcgaat tggatccggt tatcgcccgg cactcccgcg
    23341 cccatcgcct gcatccggcg ttgattcggg ccgtgatcaa aacagagtca gacttcgatc
    23401 cactggccgt atcacatgcg ggcgccgtcg gcttgatgca gctcatgccc cagaccgcta
    23461 tgcgcttcga tgtgcgggat tcatacaatc cggatgagaa tatcggcggg ggcacgaaat
    23521 acttacggca gctgctggac cgatttaacg gcaatctgcc cttggccctg gcggcctaca
    23581 atgcgggaga acatgcggtg gaacgctatc aggggcttcc accgattcct gaaactcggc
    23641 agtatgtggt caaagttctc gggtactatc gcaccttcct catgaacgcc cggacgtctt
    23701 cttcccgggc ttacgtccca caggcgcctc ggtcgcaagg acgaggtctc gctcattcga
    23761 cggcatcccg ttaacctgtc caccccctaa cagtaactgg ctatggttcc gccacccctg
    23821 ccgcttggct tcgggaaagt ccgaccgata atgggctccg acgctatttt cccgccacaa
    23881 ggcggcatcc gtgatgcagc gcgccacctg caccatgttc ttcacctcga gcgcagcgcg
    23941 cgacgaaaag ggtttgccaa gaaggcgcat ccagcggacc aactgggcgg aggcactgat
    24001 caaggaatct cctgaacgca cgagtccgac ttttccccac atcaaccgcc gcagcgaact
    24061 gcggagcttt tccggatcat cgagctcagt cggtcgcccc tgctcgagct cttcgagtga
    24121 cggcagctgc actgactcgg aaaaatggcc cgcatgggcc acggcagatt gcgcggcccg
    24181 cataccgaag accaacccct ccagtaatga attgctggct aatcgattgg ccccatgcac
    24241 gccactgcag gccacttctc ccgccgcata caacccaggc aacgtcgttg cgccatgcac
    24301 atcggtccac acgccaccca tcatgtaatg cgcgctgggg gagacaggaa tccactcctc
    24361 cgtcatatcg atgtcatacc gcaggcaggt cgcgtagatc gtgggaaacc ggcgcttcac
    24421 aaaggctgcg ccaaggtgtg tgacatccaa atagacatga cgcgcacggg tggcggccat
    24481 ttccgaccag attgcccggg agaccacatc gcgcggcgcc agtgcgccgt ccggatgata
    24541 ccggtgcatg aataactcgc ccttgatgtt ccgaagctgc ccgccttctc cgcgaatggc
    24601 ttccgatagc aggaagggag gactcgacgg caggtacagc gaggtgggat ggaattgcac
    24661 gaactccata tccatcagca acgcgccggc ccgcagcgcc attgccatgc cgtcgcccgt
    24721 cgcgttgccc ggattcgtcg tacgcgcgta cacctggccc gctccgccgg tcgacaagac
    24781 caccgcgctg gccggcagca cggtgcgctc ccccgacatt tcgttcaaca cgatggcgcc
    24841 gcaacaacgt ccgtcgacca ccgccaggtc gaccgtaaat cgtcgatcca atcgctgaat
    24901 gcggggctgc ctggctgcat aggtcatgag cgcgcgcacc atctcgttgc cggtggcatc
    24961 cccccgtgcg cggaggatgc ggcttcggct gtgcgcggct tcgcgggtaa acgcaaaccg
    25021 tttgccgatt ttgtcgaact tggcccccca ggcaatgagc tcctgaattc gctccggccc
    25081 ttcttcgacc aacacccgca cggcttcgcg gcgacagagg ccatgcccgg cttttaacgt
    25141 atcggtgaga tggcttccga catcgtcttc ttcgctgagt gcgacggcca caccgccctg
    25201 ggcatacatg gaattgcttt ccaggggatg ccccttggtc agcatcaaca cacgtcccgc
    25261 tcggctcaat tcaatggccg cgcgcaaacc cgcgaccccg ctcccgacga cgagaaaatc
    25321 tgtttcaatt tttggcgatg cactgcgggc tgacatggct agggtttggg ttggctgcga
    25381 gcgggatctt cattgaggct ggctcgtgtc cccatgccga acggcagatc gccttgagcc
    25441 tctcgaagca gaatcgattg tgtcccgacc caggccaatc ggccgtggcc tcgttggcgc
    25501 gtgccgacat cgccgggatc ccgtttccac tggccctgcc aatggacaaa cacatcgatg
    25561 ttgtcgcctt caatcaaaat gcgctcgatg gaaaactcca attgaatatc gcgaaaggtg
    25621 tcaaaatcgc tatggatatc ccgttgcacc tggtccaggc ccccagacgg gagaagcaac
    25681 gcctccaagg cgctccggtc ccgctccacg taggatttcc tgagtgactc cacggcctga
    25741 tcgatccgga gaatacggtc gtgatcgtct ttatactgaa tggtttttcc ggagcaactc
    25801 acgcccagaa tcagcacgaa accgaggaga agtcgaccaa tccagggaga acggcgcgca
    25861 gcagaagtaa acatggcgca gtataggaat cttggcggaa agaggtcaag caacggcctg
    25921 gcccttacgt gactgacttc cgatcccttc gaatgattcg acagcagaca acacgtcaga
    25981 atcttgactt gctttttgaa ctgaaaccca ctagactccc gcgcctagca gttggagatt
    26041 gaatgtatgg cgagtgtcct caagaaacgc cggaagaaga tgcgcaagca caagtataag
    26101 aagttgcgcc ggcgtcagaa attcttgcgt cgcaagagct gagcccatca cgacataatg
    26161 gacggaggga tccgtgcccg aaggaaaaaa agttcgcatc cgcgttcgaa cggtgaactg
    26221 tatctacgtc ggggatttcc tcatcccgcc catgcgtaat cgtgtgtccg atgcgatcaa
    26281 tgaagagcag cggctgttta tcagcttgac tgacgtgttg atcaatgaca aggatcgatc
    26341 ggactttgtg gcgatcaaca agaacctgat cgagtcaatc gctcagctct agcgctctca
    26401 cctcatcacg aaggtcatgt cgtcttcgta ccgccgcgac caccggtgat acgctacgcg
    26461 cctgcctccc tgaaacaatt gtcgtaacac cgatgttctc gttcaagcgc ttctacccct
    26521 tcctcaagcc ctacgtcccc cggatgatcg ctgccgccgt catggtcatg gcggttgctg
    26581 ccatcaacct tgccctcctt cgcttaggcg gcactctgtg ggacgtcatc acggtccagc
    26641 acgatgcccc gcgcatgaca aacatgatcc tgctcttgtt gggcttggtc ttgatccagg
    26701 ggttatgttc gatggggcac agttacctca cggcctgggt ctcccaacgg gtgatggccg
    26761 atttccggat ccatctcttc gcacatctcg agacgctgtc agtcaacttc ttctcgaagc
    26821 gccgaaccgg cgaattgatg tcccgcctca tgaacgatgt caccgtgatc caaaatgtcg
    26881 tcaccgatac acccatcgac gccgccaaac aaatcgtcac cttcatcggc ggcgcgggct
    26941 tcctgttcgc catgaactgg caactctgcc tgctcattct ggtgctgctc ccgatgctgg
    27001 tcgtcgtggc caaactgttc gggcggcggc tccgggcgct gtctaccaaa atccaggacc
    27061 aaacggctac cgtcagcacg ctggtcgaag aagtcatcgc agggatccgc gtcgtgaaat
    27121 ccttcgtcca aacgaaacgg gaagaagaac ggttcgtgac tcaggtccag tccgcgatgg
    27181 agctgtcact gcgccgtgcc accatcatgg cctggttcgt gccgacaatc atcttcgtca
    27241 cctttgccgc cgccgccctc gtcttgtggt acggcggccg ccaggtcatc gacggctcag
    27301 tctcccccgg cgacctgttt gcgttcgtgc tctttgccgg cattctgatc ggaccgttcg
    27361 gatcggcagc ccgcgtcttt gcgcaaatca aagaagccca aggcgcgatg caacgcgtgt
    27421 tcgaaatcct tgatacccat gccgaaattg cggacgcgcc tggcgcgatt gaattgccgg
    27481 cggttcgcgg gcacgtgcgc gcggaaaatc tgagcttcgc ctacgacccc cggcaacccg
    27541 tgctctcgga cgtgtcgttc gaagccaagc ccggtgaact cattgcgatc gtgggcccca
    27601 ccggctccgg caaaacgacg atcatgaacc tcttgcatcg attctatgat ccgacctcgg
    27661 gacggctcac catcgacggt catgacgtca agaacgtgcg gctcgacagc ctgtaccggc
    27721 aaatcgcgct cgtgcctcag gacacgatct tattcggcgg cccgatccgc gataacattc
    27781 gctatggacg cgaggatgcc accgaggagg aaatcattgc tgccagcaca gcagcccatg
    27841 cccatgagtt catcgtggga tttcctgacg gctatcagac gatcgtcggc gaaaaaggca
    27901 tcaacttgtc cggtggtcaa cggcaacgcg tcgctatcgc ccgagcgatt ctcaagaacc
    27961 cccggattct gctgctcgac gaggccacct cggcgctgga taccgaatcg gagcggctgg
    28021 ttcaggaagc cctggaacgg ctcatggtca accgcaccac cttcgtcgtg gcccatcgac
    28081 tcagcacgat tcaacgcgct gaccgcattc tggtactgaa caaaggcaag atcgtcgaat
    28141 ccggtaccca tgcggcgctc ctggagcaac aggggctgta ccactacctc tacacactac
    28201 gactcagcga actcccggtg taatcgatca ggagaccctg atgaacaggt cagtcttcgc
    28261 actcgtcttc accgcactct tcctctcctg gaccaccccc ggttcagccc attcgccctt
    28321 gcccaagccg gaccatgtgg tgatcgtgat cgaagagaat catgccttcg gccagattat
    28381 cgactctccg gccgccccat acttaaacgc gctggcacgt aaaggcgctc tgctgaccaa
    28441 ttcctacgcc gtaacccatc cgagccagcc gaactacatc gcgctgtttg ccggcaccat
    28501 ggaaggcgtc aatgggaatg tctgtccgac cccgctgacg gctccgaacc tgtccagcac
    28561 tctgaagcag gccggacagt cgtttattgg ttactccgaa gatctccccg ccgtgggggc
    28621 cgtcgattgc attgcaggcg cttatgcgcg gaaacataac ccctgggtga actggcaagc
    28681 gtctcctgtc aacaccgtcc cggctgcgca aaaccgcccg ctgaccgact ttccgacgga
    28741 tttgagcacc ttgccaaccg tcagcatcat tgtgcccaat caacaaaacg atatgcacga
    28801 cggcaacgat cccgaccgca ttcaacgcgg agatcactgg ctccaggtcc gcctcgaacg
    28861 ctacgtagac tgggcgcaga cccacaacag cctgttgatc attacgtggg acgaggacaa
    28921 cggaaaatcc gacaaccata ttcccacgat tctcgtgggt ccgatggtgc gcccaggacg
    28981 ctaccatgag cagacgaatc actacgggct gctgcggacg atcacggaca tgtatggagc
    29041 gcaaccggtt gggctcagcc ggcaggcctc cccgcttacc acgatctggg cagcgcccaa
    29101 actcacgccg tagacgtgcg cgcggccccg tttcgcactc gactccaggc cttccacagt
    29161 tccatcacca cgaacggcag cattccgcac gccgcgacca accaccagtc atcgagtcgt
    29221 agcggaacga ctcgaaagat atcctgtccc catggactca acagaatccc cgcctgcagg
    29281 agggctgaga tgaggacggc ggccaccagc atccgattgg tggtgatgcc aatctgaaac
    29341 agggagaacc ggtcgtgacg acaggtaaac gcatggagga actgaaccag caccagcgtc
    29401 gtaaaggtca tggtgcgcgc aaacgtgacc tcgtccttca tgatcaccag ggcaatcccg
    29461 aacaccgcca aggtcgccgc cgccatcacc gcgccttgaa cgcacacggt cacgaaccgt
    29521 tggcgatcca gcagcggcgc tttggggtcg cgcggtgggc gcttcatcac atcgggatct
    29581 ttcggatcca cggctaacgc gagcgcagga aatccgtcgg tgaccagatt gatccagaga
    29641 atgtggatag gcagcagcgg caacggccag cctaacaacg tgctgcccag catcaccagc
    29701 acttcactca ggttgcacga cagcaaatag tgcacggctt tgcgaatgtt ctcgtagatg
    29761 ctccggcctt cctcgaccgc cgcggcgatg gacgcaaagt tatcgtcggt tacgaccatg
    29821 tccgacgcgt ctttcgtcac atccgttcct gtcatgccca tggcgatccc gatatccgct
    29881 tcccgcaccg caggggcatc attcacgccg tcgccggtca tcgccacgac tgcgccttgc
    29941 gcgcgccagg ccctgacgat gcgcagcttg tgttcggccg acacgcgcgc atagacacac
    30001 acgtcacgca cccgggacga cagctccgca tcagtcaatc cgttcagttc caggccggag
    30061 agggcctgcg tcgagccgga tgcgaacccc gcctcgcgtc cgatagccaa tgccgtctcc
    30121 ttgtgatccc cggtaatcat cacggtcgtg atgcctgcgg accggcatcg ctcaaccgct
    30181 tgtttcgctt ccggccgtaa aggatctttc atcgcagcca acccgaggaa caccaattgc
    30241 tgctcaatgc ctttcgactc gaatgtcgcc ggatccggat cgagtacgcg ttgcgccaat
    30301 gcgacaaccc gcagggcttg ctgagcgaat tgctgattga tcgataccat cgaacggcgc
    30361 attgaatcag acaggggggc gacttccccc gacttcgtca tatacgaatc gcatcgctcc
    30421 agcaggatat ccggggcacc cttcacgtag gccacggcac gcccttccga tcggcgaatc
    30481 atcgtcatca tcttgcgttc cgagtcgaag gggatttctc ccaccagagg gtgcgcgcgt
    30541 tcgagtgctt ctttccgcca tccgcccttg ccgcccgcaa ccaacaaggc cccctccgtg
    30601 ggatcaccca ccaccgtcca gctgccttct gcggcccgca acgaggatcc gttgcataag
    30661 actgcgctcc acaacagtgc tcgcaacgct cccgtgcggc catcgccatc gacaatcgtg
    30721 ccctccggcg catatccctc cccggtcacg tcatagacca cgtcatcgac ggccagccgg
    30781 gtgaccgtca tttcgttctt ggtcaaggtg ccggttttgt ccgtacagat caccgtcgcc
    30841 gcgcccaggg tttcaacggc cggcagacgg cggatcagcg catggcggct caccatacgc
    30901 atcacgccca gggccagcgt cgtcgtcaca atcgccggca gcccttctgg aatggccgcc
    30961 acggccagac tcacggccgt caagaacatc tcgaacagcg gctccccccg ccacaagcca
    31021 agcccgaaca ccacgaccac gatagccagt gataaccaca acaacacatg gccgaactgt
    31081 tccaagcgcc gctgaagagg tgttggctcg acaggcaccg tggtcatcag cgtcgcaata
    31141 cgccccagct cggtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacgaat
    31261 cccagcaacc cgttcagcag cacgatggcc aagatggcgc acgcgtccac ccattcgccg
    31321 agcaaacccg acacgagagc ggcgccgatc agcacccaga tgatcaggct gctgaattgc
    31381 gcacccaaca tatgccaggg cgaaggcggt gcagcctcag gcaattcatt agggccatgg
    31441 gcggtaagcc ggcgcgcggc ttcatcttca tccagcccta gcgcctgatc gacgccgaac
    31501 tcccggccga gatcgtcaag ggaccgtgcg taccaccggg tctggctatg ctgtcgggct
    31561 tcgaccatac gttccgatct ctttcagctt ccctgcgggg gcggctcctt agggatagcc
    31621 cccttccccc aaccccaatc ctaagcactt caacttgcgg agaaaacagc cgttcctgcc
    31681 ctcgcccacg cggcggcgag ggggagtttc gtcgatttca caatcccggg gactcaatcc
    31741 cggcgcaaac cagccgatac agtatccacg aaggacggag agtggccctg aagggggcat
    31801 cattcctgtg gtaggaacgg ccgtcgcttg aagctgtgga ttgcgtaggg tgggtgatct
    31861 atgacacgtt cctcctggtc gtcttcgaat ccgcttccgg ataaccttcg tgccagcctg
    31921 aaggaccttc gtcacggctt gctcggcctg cacaaatccc tcatcgtctc cgaacagctg
    31981 acgtatgaac gcatctatgg gcgcatctcc tcggccggtc agcttttgca gctggtgatg
    32041 aatgacccgt ggttcgcctg gctgcacccc ttgtcgcatc tcgtcgttcg catcgatgtc
    32101 gtgctggaag atcacgaaga cacgaccatc gaaacggcgc aagaccttct gctggaagcc
    32161 cgccacatgc tgcgcccttc agaagaaggc gacgggttcg aacggagtta ttacgaagcc
    32221 ctgcaacgca ctccggacgt ggtgttggcc catgccagcg tcaaaaagct gctcagcacc
    32281 gcggccactg ccgcctcggc ggccgcagcc tgaccgcctg gcccgtctcg atcagaacag
    32341 cggatggtgc ggggatgagg agaagggcgc cgaacgcggt gcatcacctc gatttcccag
    32401 aaaccgccat ccctgcctgt cccgcacgag atagtgcacc tcctgaaacc agctatccag
    32461 cgtgatgcgc gcgcctgatt gcacgtcagc gccatacaag ccgccagtgc aggtcaactc
    32521 gacgcgtaac ccgtcctcgg cacgcacgat cttcatctcg gaaaagagat gcgtcgagga
    32581 caatgtccga tagtgctcga acacttcacc ccagatgcgc tggacatcct ccacgtgtaa
    32641 gccgtgatag ttgtagcccg gcgcataaaa ctgcatcagc gaggacaggt cctgcttctc
    32701 caaggcacgt tctgcacgat cgaatgccgc cagcacttcc tgcagcgtgg gatcggcacg
    32761 aaccgccttg gcaccggtga cggtcccact gtcacgcgtc acggtcatat caggaagtac
    32821 gtgcaccacc gcaggagcag aggatacttg tgccatcatg gccacagcaa cgatcccgaa
    32881 gaccgtccgc caccctacac gagcgcctcg cctcgtcccg atcgcctgta cgtctcgttc
    32941 gcgcatcgcc acctctctct tcacagcatc gtgccttgtc ccaggatagg gatacatcct
    33001 tatcgaggac tatgcaacgc cgataccgcc attgattgat ggtggcccgc gagatattcg
    33061 ccacacggtc actccctgag gtgtgccgtg ctgccgtcaa aggcgacagt cctctcctga
    33121 tcctgtggca cgacgccaca gcatcaaccc gtgtttccga tcgaaaacca atgatgctca
    33181 gcatagagga cgctggtttt gtacttttgc tttggtccgc aagacctcga cgttagacta
    33241 cagtgacgcc acaatcagct ggcagaaaga gcggacaggg agcgggaatg ccatcggcaa
    33301 tgaaacagtt gcttgcaagt gcactgggct atgctctcat ggcatccggt gccggtctcg
    33361 ccgaggagat tccgcatcag cgaagcatcg ccctcatcga caagacctgg gccgtgagtc
    33421 tcgacatgac cggctatcgc gtccaagcgg atggcgtcaa accggacagc cggcgctatg
    33481 tgctcgccac caacgacgca gccgccatgc agctctccgt cacgctggag gccgtctccg
    33541 gacaggccac cgaacagggc tgcctcgcgc atttgcaccg tatcgcacaa acagctgcgg
    33601 caccatccca tgcaggtccg acacgaaccg agatcaatca actcccggtc ttcgaatatc
    33661 ttctgtatga gaccagcgcc gggtggacgg accaggttca cctctttgcc tgtacgaaca
    33721 aagacaacgt ctataccgac attcatctgt cacacagcgg caccacaaca gtagacgcct
    33781 cgcaacttcg gaaggtgctg gagagccttt ccatcgttgc ggcggcccag cccggcagcc
    33841 tggattactt tcgtgcaggg agcgcgccgt atctgcaggg acaattcaag caggccattc
    33901 cgcactatga acgggccgtc gcccttgagc ggacgaaccc ctctttggac aaggctctct
    33961 ggcgtctgct cgtccataac ctgggtgtcg cctatcgcag gagcggtgat ctcgcccgcg
    34021 ctaaaacaac gatcgactat ggtctttcac aagagcccgc gaatccatcc tttcactatg
    34081 atctcgcccg cacctacgcc ggaatgaatg aacgtgaccg ggccatgcaa tctctgcacg
    34141 acgcatttct gcacttccgt cgcagcaacg accgagaacc cataccagac ccccggcagg
    34201 atgtatcctt cggtcggttc atgctggatc ccgcgtttag aacattaacg gaatcgctca
    34261 tgcagccagc tatctaagga ccgtgcgaac cctggaagac ccgccgatta caaaaggacc
    34321 aagggatgtc aaaacgtaag aatacgcgtg tcgagatgaa tcggccgatc gatgtgcaag
    34381 gcccccgtgg aaccagccag ggtgtggtga aggatttttc acccggcgga tgcaagattc
    34441 aacagactgc cgccaaggtt cactgcggga tgcgattgac gctgcggatc tcgcttcccg
    34501 atcgcatcga accgatcgaa atcaagcccg ccgtcgtcac gtggacgagt aaggatgcct
    34561 tcggcgtgga attcctggcg ctttcggcag aaacgcgcag ccgggtcaaa ctcgtgtacg
    34621 accttcttct ggacgcgcag tcgacagccg aaacggaacg ggtcatttct cttcccggcg
    34681 tctcctggaa ataagttccc tgcctcgcac aaccaccgag taccagccag aatcttgagt
    34741 tctgccctgt ttggccatga cgcatgggtg caatccgtcg cccagtggcg cgcggggcaa
    34801 aacgcggggc gcggtacgaa gaagacgaaa cactctcgaa ggagatcacg caagcggatc
    34861 ggtgaacgac catccacgcg caatagatac gagctcgtta ctgcgcagga gcgggcggga
    34921 ttaattcggc cactttttcg ataatggcct gcggcataaa tggtttttgg aggtaggctg
    34981 cttgtgcatc cactccatga gataccaaca tgtcaccggc atatccggag atatagagca
    35041 ccttgatctt gggaaacatc gtcctggctc cctgcgccaa aaccgaggcc ttcatacgtg
    35101 gcaggatcac gtcggtgatg aggagatcgc agccgccctt gcgtagttgc aacatctgca
    35161 gcgcctcgac gccgtccgcc gccgccaaca cttcataccc ctgatcacgc agcacggccg
    35221 ccacaagccg tcgaatgccc tcatcgtctt cgaccaggag aatggtggca caagtcgccg
    35281 gaagcggtcg tgaggatgcc ggctgttcag cttccgatgt ttgcgactgc acgcgtggca
    35341 acatcaccgt aaaacgcgta ccccgtccag gttggctggt gacatcgata tacccgcggc
    35401 tctccttcac gatgccatac acggtggcca gccccaaccc ggttcccttt tcgaattcct
    35461 tcgtggtgaa aaagggttca aaaatatggg cgagggtctc ggcatcgatc ccgcatcccg
    35521 tatcttcgac caccagggta acgtaaggac cgggaaccgc gccgggatga gtgcgcacat
    35581 actgctcgtc gacatcggta ttgccggtct cgatcgtcag aatgccccct tctgccatgg
    35641 catcgcgggc gttcagggcc agattcagca atacctgctc catctgcacg gggtcgccca
    35701 agacgtggcc cgcctgggga tggagcacaa tcaccgtctg gatctgctca ccgatcaatc
    35761 gacgcaaaat gtcttccatg tcgcgaatca gggtgttgag cggcacgtca cgctgctcga
    35821 gcacctggcg tcgactgaac gtcaacaatt tcttcgtcag cgccgcggct cgcgtgccgg
    35881 cctggccgat catttccact tcatgatgca gcggatggga tcccagttgc tgcgcgactc
    35941 ggtgcgcatg gccgatgacg accatgagca aattattgaa gtcatgggcg atacccgcgg
    36001 ccaatcggcc gagcgcttcc attttctgcg attgccgcag gctttgttcg tcctgtttgc
    36061 gcttggtcag atcgatgatg gtttcaatga catggacacg gccgtctttt gtcggggcct
    36121 tcgcccaatg caccagtaaa tggcggccgt tcggcatttc cacctgctca gagaccactc
    36181 catctcggct caacgcctca ggcatgcggc agaattcgca cggcgccgtc tttcccgcaa
    36241 ttttttcata acagggcgag ccggccatgt cgccgaacgc tcccagaccg gtctggtttt
    36301 ggaactgcac ggaatggtcg gcaggatcga tcacggtcac gatgaccggc tgggaattca
    36361 ggagcacctg cggatcatac gagagaccat tgggggagtg cggagaaccg ggcatgggcg
    36421 tcctcgtgcg gattgggcgt ggaagaatgt tgaacgaata ccctctctct ggttgtacta
    36481 catcaccacg tacagtcaag gagatgatcg attgtctggc ttgggcggta cgtaggaatt
    36541 atggaagcgc gtcgcgtcgc ctgatcagcg gccgttattg acctgccggt aaaagaacgt
    36601 tttcgccagc tccgccgccg cgacataggc caggagaatg ccgagcagcg cgacccaaaa
    36661 gacagccggc aacggttcaa aacccagaaa tgaacccatc ggcgttatcg gtaacaggat
    36721 cgtgaccagt gcaatcagca acgttgacca caacaagggc gcagacgggc ggctggtcac
    36781 gcaagggcgg cgcgtacgaa tgaccagaac aatcaacgac gcggatagca ccgattctac
    36841 gaaccagccg gtacggaatt gggcggtcgt ggcgtgcagc aggaacaaca agatgccaaa
    36901 cgtgaggtaa tcgaagaacg agctcaccaa tccgaaggtc agcatgaacc gccggatgaa
    36961 cggaatatcc cagcggcgcg gtcgatcgag caattcagag tccacatgat cggtggcgat
    37021 cgtcatttca ggaatgtccg tcaacacatt cgtcagcaag atttgtttcg ggagtaacgg
    37081 cagaaagggc aagaacagcg aggcaccggc catgctgaac atgttgccga aattcgcgct
    37141 tgtcgccatg aacacatact tgagggtgtt ggcaaacgtg cgtcgcccct cccgcacgcc
    37201 ttccaccaat acgctcaaat catgttccaa cagcacaaga tcggcggctt ccttggccac
    37261 gtcgacagcg ccatcgaccg aaaggcccac atccgcggcg tggagggcag gcgcatcgtt
    37321 aatgccgtcg ccgaggtagc ccaccacatt cccggaaccg cgtagcgcac ggatgatgcg
    37381 ctccttttga ttcggttcaa tttccgcaaa aatgtccact tcgttggccc gggcccgaag
    37441 cgcgtcgtcg gtcatgcctc gcaagtcact cccggtcagc aagcgcggat tggccaggcc
    37501 gacctgtcgc cccacatggg ccgccaccag tccttgatcc cccgtcacca tctttaacga
    37561 cacccccagc ttgcggagca gcgcgatcgt gtcgacaata ccggccttcg gcggatccac
    37621 aaacactagc aggcccagga aggtcattcc cgtttcatgc tctttggaaa cccggtcgat
    37681 ggctcccaaa tcccgatagg ccactccgat ggtacggtag ccctcgctgc tgagcccccg
    37741 cacctgctcc ctgatcgatg cgcgaacctg atccatcggc atgagggacc cgttctgctg
    37801 ctcggcatgc agacacaccg acagcatact ttcaaccgcg cctttcgtaa tcagcagatg
    37861 ggtctgcgga gtggcgacca acaccgacag ccgcttccga acaaaatcgt acggttcctc
    37921 ttccagctta cggtagctgc cgagatcgaa ggaacgatgc tgccgcaggg cctcatccaa
    37981 gggattcgga aacccagttt cgaacatcgc gttcatgtgg gcatggaaca gcacgcgctc
    38041 gctcggctga ccatccagat ccagcgcggc atgcagtcgc atggagcctt cggtcaaggt
    38101 ccccgtcttg tcggagcaca gcacgttcat actcccgaaa ttttcgatcg acgccaggcg
    38161 cttgaccacg acgttctgtt gggccatgcg tttcgcgccg tgcgagaggt tcacactgat
    38221 gatggccggc agcaactgcg gcgtgagtcc caccgccagg gccatggaaa agaggaagga
    38281 ttcaagggcc ggccgttcaa gatagacgtt gacggcaaaa atcgcgagca cgagcagcag
    38341 cgtcacttcg agcaggagat agccgaatcg ccgcacgccc cgttcgaatt ccgtctcggg
    38401 cgccctgagc gccatgcgat gggcgatgcg gccgaattcc gtgtcggttc ccaccgccac
    38461 gacgacaacc cgggcttgcc cgctcacgac atgggtgccg agaaacagac tgttggtacg
    38521 cttcgccaac ggcgcatgag ccggtagtga gcctaccgac ttctcgaccg gatacgtttc
    38581 tcccgttaag gtggcctcat cgacgaacag atccttcgcc tccagcacca aggcgtcacc
    38641 cggtaggctc gacccggcgg aaagctcaat gacgtcgcct ggcacgatgt gatcggccgg
    38701 aatgtcgcgc agctcgccat cccgccatgc ccgagccgtg atttgcacaa gagctaatag
    38761 gccggccact gcccgggccg cgctatattc ctgccagaaa ctcagcaggg cactgacgag
    38821 gatgatggtc aggatgatga gcgcatcact atgctcagcg agaaaaaacg agaccccgga
    38881 agcgaagagg agaatgagaa tgatgggact gcgaaactgc gccatgagca agcgcagcgg
    38941 ccggctattg cgctgaggct tcagccgaac ggatgcagag gtggcctgcc gcgcgtcaac
    39001 ctccgctgtc gccaatccat ttggtgtggc ggacaattgc cgcaacacat catcggcgga
    39061 aagagaccag aatggtgtct gcttgagcat ggttccacac cggcatcggg ctacacccac
    39121 tcgggagcat gttccttcgt cacgtcctgt tcggcaaacg tacggtacaa ggcagggagc
    39181 accaagaggg tcaaagccgt cgacgtgaac aggccgccga tcaccaccgt cgccaacggc
    39241 cgctgcactt ccgcgccgat cccctgcgcc aacaccagag gcagaagccc cagaagggtc
    39301 gtcatcatcg tcatcacgac cggtcgaagc cggaggacgc agcccgagat gattgccgcc
    39361 tctgtctgct gcccttcgtt ccgcaattga ttgatgtacg acacgagcac gatgccattg
    39421 ccgaccgcca agccgaacag ttcgatgaag ccgatcgagg ccggcacgct caaatactgg
    39481 tcgctcagcc acagggaaac cacgcctccg atcaacgcga agggcaggtt cagaatgatc
    39541 aacccggcat accgcagcga atggaacgcc caaaagagca gaaagaacac caggccgagc
    39601 gtgatcggca ccaccacaag caaccgcgca ttcgcccgtt ccatattctc aaatgcgccg
    39661 ccccaggcca cggtataccc ttgcggcaat cggagctgct cggccaagcg cttccggcct
    39721 tcatccacga cgccgccgat gtcccgcccc acaacattga acccgatata gatccgccgc
    39781 ttcgcctgtt cccggctgat tcgcgccgga ccttcccgca tctcgatcgc cgccagttcg
    39841 ctcaaaggaa tcggggcacc ggacgccgat cgtactcgaa tatctcctat cgcgccgata
    39901 ctgttgcggg acgattcagg aaatcgaagg accagttgaa accgccgctc gccttcatag
    39961 acctgggtcg ccggtcttcc gcccaccgcc gtgctgatga tgtcttgcac atcggacacg
    40021 ttgatcccgt atcgagcgat cttttctcga ttgatatcga tcgtgaggta cggctgaccg
    40081 gcgacctgtt cgaccttgac gtctttgact ccgtcgatcg tccgcatgag atcggcgatc
    40141 cgttcggcat gctgatacag cagatcgaga tcgtcgccga acaacttgat ggcgcattcg
    40201 gtcctgatgc cggagatcag ctcgtccacc cgctcctgaa tcggctggct catcagcacg
    40261 gaaataccgg ggatttccgc cagacgtgtc ctgatagcat cgaccagctt ggcttgcgtc
    40321 tgcgcggtga tccacgcctc acgcgggcgg agcgccacga tcgggtcgct ttcattgggc
    40381 tcttccggcc ccaccgcgat gtccggccgg ccgatcttac tgactgacat ccgcacttcc
    40441 ggaaactcca gcatgacttt ttgggtttgt ttttctatgg cgatcgattc gtccaacgac
    40501 acactcggca ggcgcacgat ctgcggggtc agtgccccct cctccaagat cggaatgaat
    40561 tcgcggccga cggaaggcaa caggctcagg ctggccagca cgatcgccgt tgaactcagc
    40621 agcacggcgg cgcgatggtc cagcgtccac cgaagcaccg gttgatactg cgccttcatc
    40681 cagcgggtga gacgagtctc ctccggatga tcgccgtgta agatcagaga ggcgaggacc
    40741 ggggacaggg tcagtgtcac gatcaccgag gccaacaggg aaatgacgag tgtataggcc
    40801 aggggcgcga acattttgcc ttccatgccg tgcagggtca tcaagggcag aaagacgacg
    40861 ctaatgatga gaatgccgaa caggatcggc cgcccgacct cattcgtcgc ccgcagcacg
    40921 acctctaagc gggatcgttc ctgcagccgg ttctctgaca agtggcgata aatattttcg
    40981 accacgacca gcgagccgtc ggcgatttct ccgatgccga ttgccagtcc gcccaaggtc
    41041 atcaggttgg cggagagtcc cacacgctgc atcaccacga acgtgatcaa cggcgtgacc
    41101 agcagcgaga ccgtcacgac gatcgcgctt cgcacatgac cgaggaagag aaaaaagacc
    41161 agcgcaacca gcacgacacc ttcgatcaat gcgtcgcgca cggtgtggat ggcggccgtc
    41221 acgagctcaa tgcgatcata aaaaggcacg agggtcagtc cgccgggcag aattccctga
    41281 cgctcgatgg attccgcccg ttgtttcacc gcctgaacca cttcacgcgc gttgccgcct
    41341 cgcagcatca acacggtgcc ggccaccacc tcccgttcgc cgttcagcac ggcggcgccg
    41401 tggcgtaccg catggccgat gcgaacctcc gccacatcac gcacaaacac cggcacgccg
    41461 cccgcctcct tgacgacgat ccgctcgata tcggccaggc ttttgatgag gcccaacccg
    41521 cgcacaatgg cgcgttcagc atggcgttca aggacatttc ctccggcgtt cgcattgttg
    41581 cgttccacgg catcgaagac gtcatgcagc gtaaggctgt acttgcgtag cctgtccgga
    41641 tccaccagga cttgatactg tttcacgaac ccgccgaggc tgttcacatc aatgacgccc
    41701 ggcacgccct tcagcagggg acgtagcacc cagtcctgta tggtccgctg gttcgtcagc
    41761 gcttcttcca ccagcgccgc attcgtatcc ggcttcgtcc cgccctcgag gtaatactgg
    41821 tagatttctc caagcccggt ggtcacgggg gccagaacag gctccatgcc ggccggcaac
    41881 cgctcgcgca ccgccatgat tcgttccagc acgagctgcc tggcaaagta cacatccaca
    41941 tcgtcctcga acaccaccgt cagctgagag agcgcgaact tggagatgga gcgaatttcc
    42001 gtcaggcccg gcaatccgtt catttgcagt tcgatgggat aggtaatgaa ccgttccact
    42061 tcgaccgggg agagtccagc cgcttccgtc agcacctgca cttgcacgtt ggtcacatcg
    42121 ggataggcgt cgatgggaat cgactggaag gcgaagacac cggccgcggc gcagaagcag
    42181 gccaggccga gaatgaggat tcgttggcgc agggagaatt cgagcagcgc agcaatcatt
    42241 gagtgggctc gattttgtgg atatcgagtt cagacttgat cgcaaaggct cccttgacga
    42301 ccacgtcctc cccttcccgc agcccctcca gaacaatgac ctgcccatct tgttcatcgc
    42361 ccaactgaac gcggcgcggc gcgaatcgat tctccccttg ccgcacgaac aacattttgc
    42421 ccccgccctc ctgctgtatt gccgacaacg gcacagtcag cacagcgggc tgtggcgagg
    42481 catcgacgcg aaccatggca aacatttccg gcttcaacgc acgatccgga ttcggcacgg
    42541 tcacacggat gcgcatcgtt cgggtggtcg gatcgagcac atcgctgatg taggtcatgc
    42601 gcccggagaa caggctatgg ggataagccg cgacgatgac atggaccgtc tggttgggat
    42661 gaatgaactg cacgtctttc tccggcacac tggcgatcac ccagacatct gacaagtcgg
    42721 cgatggtgaa acagttccgc gaggtttcca cgacttcgcc gcgcgtgaga tttcgcatga
    42781 tcacgcggcc cgcgaacggc gcccggatcg cgatgtcgga acgaatggtt cgctcccgtt
    42841 ccagccgctg aatctcctgg tccggcacgc ccagcagccg gagccgatgt cccgcctctc
    42901 gggcctcggc ctgcgccgtt ttcatttccg cttcccggcg ttgcaactca gccagactga
    42961 tggcacgatg ctcatgcaga ttggccgccc gttcatgcgc tagctgcgct tcatgttgcc
    43021 gcgccgtcgc cttgagatac accccctcgg ccatgcccag atcagtgctg tcgagcaagg
    43081 ccagccgctc acccttcttc acatccttgc ccgcatcgac caatacgtct accacgcggc
    43141 cccgaatcaa cgtggtgact tctgccaatt cgttttcatt ggcttgcacg gtcgccggaa
    43201 attcgcgatg caggacaaag ggttcttttg ccgctctctg cacctgaatg ccagcccgcc
    43261 cgatttctgc cgcggtcagt tgtacgattc catcagccgc tctgctcaca ggcttagcgg
    43321 ccggcggcac ccccggatct cgatcacacc cgacctgccc catcagcagc actaccagga
    43381 gcgcgcgacc cgcgaggtga agcgaacagg ccggccgcgg aagcagccgt ctgaactcac
    43441 atgtgagagc cgccactcga tcggtcatgt gtccattgtc tgttctgcat gtccctgccg
    43501 tgtataacca cagcagggac catgccgcgg gcgagccgaa atttcacaag gaatgacaca
    43561 cccatggcag cgttttgcta cgcttgcggg agggatacgg ggtgaaatgc cacagcggtc
    43621 ttctgttctc caagatggcg ccgctggaga gtcgcacctt accggacgac gagaaccgaa
    43681 caggtactgt gctgcacgac cttggtggag acgctcccca acaggaagcg ggcgaccgct
    43741 cccatcccct tggcaccggt cacaatcaaa tccactttct tcttggatgc cactttcaag
    43801 atctcgtcgg cgggtttacc gagttgcacc acttcatcga ccacataccc ggccttgatc
    43861 aatttgttgg cacattgttc caccagacgc gcaccggcct ctttgagctc cggatacttc
    43921 aggaacggca tcgcatgcat caccaccaca tcgatcggct caaatccttc ccgggcttcg
    43981 ggctgaagct tggtcaggag aaaccgtaag gccttctccg atgccttgga tccatccgcg
    44041 gcaaagagaa tacggctcac cgggcgcggc tcttccttga cgatcagcac cgagcagggc
    44101 gcatgcaggg tgacctgcgt cgagacgctg ccgagcataa accggtcgag tgcgtccagc
    44161 ccgcgactcc cgattgccac gatgccgtcc cggcgaggcg cccgatccag aatcgtggga
    44221 cccgccggcc cccgctccga ctgcaccttt cccttgagct tcaaggaagc catctgggcc
    44281 ttcgcttcgg tgatggccgt ctttcctcgt gcttcaatac gcttgatttc ttcctggata
    44341 aacggctcgt tgccgatgac caccggttga aacatgaacg gcgcgcgcaa ggcttccaca
    44401 tccgtcacat gcagcagagc cacttccggc ttctcggcaa agggcattct cgccacccac
    44461 tcggtcgccc acttgccgta cttcgatcca tccgtcgcaa tcaatacctt cataggttgc
    44521 cccttatcat tcgatctgtt gccaagaata cagaattgtt gagcaaaacc tgcgccacgc
    44581 tgccgcatct tcgcctcaac cgggcgacgc gtcaatagca tctttggcgg cagcgatgaa
    44641 aagatctggg caaacgaggt tgattcaagg acggcatcac cttgttgacg ccgaccggtg
    44701 gatgttctgc caggacagcg ccgtaaacca atggcgaaag gaagagagct tgtgggctgg
    44761 aggtactcgc agaaagagat ccgattggta tcagatcatg cgcccataga ttccggaaga
    44821 acgcggagcg gtgcagccga tgtgttcttg ccacgcgaag cggttctcat ccggccgcac
    44881 ccccttgttc cgggtcatgc gacacagcag agaccagacg gagcgcagga agtcccaact
    44941 gaagacggac ggccgtgcct ctttcgcgcc ggcatggcac caggcacgcc acatccctgc
    45001 ccgcgtcttc cgtagcgatc cgactcgagc ccgccgctgt tgaatgttca ctcgtcatcc
    45061 tccttcctgt caccggagag caggcgcacg ccatgggcat gcgcaccaat ctcctgatca
    45121 tgacggtaac atgccattgg caatcctccc aaacagaatc gaatttgatc cgaccagccc
    45181 tcggtcgcgc tgacgtgtta ctacgcaggc cctgtccctc cggttgattc ccaccgcgat
    45241 tgaaggcaca ttgtgggctt taccttggag ggcatgctgt gacgcaggct ggcgaaccta
    45301 ttcgcgtaga actggtggat tgggtccatg ccgaacaact gatccgccct atccgcgaga
    45361 ccgtctttat ccgggagcag gcggtgcccg aagagttgga gtgggatgga ctcgacccgc
    45421 aatgcgtcca cgtgctggcc tggaataaac agggtgacgc catcggcacc gcgcgcatgc
    45481 aagcacaggg cacgatcggc aggatggccg tcctcaaaga ttggcgtggt cggggcgcgg
    45541 gccgtgcgtt gcttgaggcg ttactcgagt gcgcagtgcg acaagggctc acacgcgtga
    45601 cgctgtcggc ccaaactcac gcgatcgggt tctatgaacg tgcgggattc cacgtgatcg
    45661 gcgagctatt catggatgct ggtattccgc accggaggat ggtgaaggag ttgatccggc
    45721 aggagtccga atgagtggcc cgcaggcatc cactcgcgtg agccgatggt ctccattgcg
    45781 tttttcattg gaaaagccgg cgacctacga gacactttcc cggtatggcg acgacaccgg
    45841 acatctcacg cgcatggccg ccggacgacc actattctct ccttcaacgc tgccgctcgc
    45901 tctatcatga gctgtggttt cgcctgtcgg aacgtatccg gtggtcgaga cagccgtatc
    45961 atgaaaaacc gatcggctgc cttgccggac tcacctcctg gcagatcgcg cgcatcaatg
    46021 aattgcaggt gcgatatgac ctgcgcttcg aaactcacta cggccagcat accgcgctgg
    46081 ccaactatgc ctatctcgat ctgttgcacc aggcatggac ggacacgaac cggccggtcc
    46141 ctcagggagg cgtcgttgcc gatgtgggct gcgcaaactt ttggtatgcg cgcaccttgc
    46201 acgcgttctt ccgtcctgca acgttgacgg gtgtggatgt ggagggattc cggctctacc
    46261 cgacggggta cagccgttat gatgccgccc gtggatatat cgcggggctt tcgcagactt
    46321 catttctggt cgcgaactat tgcgaggtgg agaccaaggc ggacgtgatc accgcctggt
    46381 ttccgttcgt gacaccatcg cctgtactgg cgtggcgact gccactcacg gtgttttccc
    46441 ctgaacgact gttcgacagg atcgctcgca atctcacgtc acaaggtctc ttctttatgg
    46501 tgaatcaggg gagcgacgaa gcggccatcg ccgcggatta tggtcgcagg gcaggacttc
    46561 ggctgataga ccggtgggtt catccacggc ccctccgcac gaggccacat ccccctgttg
    46621 tcacctggtg gaccacctag cctcccgctc ccggtcgcgg gagtgcctat cacattgact
    46681 catggatgga gactcattta cgacgcagcg cttcgcgatc ggacagtgag gtctgagtca
    46741 acttcacgat attgacgatc tggtcgtcga gatcgacttc gatacgcacg aggtcaccct
    46801 tcgccaacga ttcccgcttc atgcccacag attgggccac ccggaacatg cgcacctgtc
    46861 catcctgcgt tttgaacgtg aggacttgat tgcgtgaatc cacatgctcg actgaactga
    46921 cgggaaagac cactgcagtt tgaaatgcct gagccaggct gactagtccg atgccccaca
    46981 caaacatgag agtcgccagg attttccgag tcatcatgac ctcctctccc gtgccatggc
    47041 acgaccacac atgagttctt tgccggactc cgtcactgta tcgccggtac gtgggggcgt
    47101 ctgtcgccga tggtgctaat tccgggtgga atatcggccg cacggtgagg tcgtctgtgc
    47161 tacgcaaaag ccttatggcc ttgcgagaga aagaaggagc aggcatgaca gtcgcgggaa
    47221 tacaggagac tcacgcactg aacaccagat ggctaccggg cgaaaatcag gatgctgttt
    47281 gcgggcagga cgaggccggc ggagtaccca aaaccatgaa gcggctgcgg gttggccagg
    47341 acgcggccgt tattgcccgc cacgccggca ttgacccaat tttcatacac atcgctgttg
    47401 aacacttcac gccaggctcc ctcgccaggc aagccgattt gatatccgac acgagtgaag
    47461 ttggccaggt gcagaatgac catcacgtcc tgaccttccc cctccaccca acgatggaag
    47521 gccagcaccc gattctggtc atgcatatgg gtcgcacgga acccacggcc gcgcaatgca
    47581 ggcaagagac ggcgtaaggc caacagttcc cgcgtgaacc gcaggtgatc gagcatctgc
    47641 ctgtcgccct gatcgaggcc cgcccaatac agcagcagat cgcggtggga cacaaagtcg
    47701 tccgaccact gtttatcctc caagaactct tgtcccatga ataacatcgg aatccccggt
    47761 gccgtcagac tgagaccggt cgcgacacgc gcccgactcc tcgcaaacca ggaccgggga
    47821 tggtccggat cgcccagccg cgctatacgc gcttctcgac cgttatagac gatgtcgtga
    47881 ttctccggcc cctgcacgaa acgccatgac tcagaaaaac cctgaggcca cagactctgc
    47941 gccagctgcg tcatgtggag cggccgttcg tccggagcgc tggcgttggc aatcacatcg
    48001 cgtatggcaa ttcgcagtcc atcggtcaac gtcgtatcga acccggcacc aaccggcggc
    48061 ggcgcgatga taaagggatt cacatcccaa tattcagcat ggtgcaaggt cttcggtcgt
    48121 tgcgcatgca cggtggaggt gaggtcctgg cagaaacgcc acccgtccgg ggcaccatcg
    48181 tgatcgatca cgctcacctg gtcataccgg aacccatcga tccggtactc gccgagaaaa
    48241 aatttggcgt tctgaatcag aaagtcgcgc acctccggct tggaaaaatc gaataccagc
    48301 cctcccgcgt ggccccggtc cgtaaagtag agagaattcc actgaccgcc ggcgggatcc
    48361 tgccggtcga aaaaatacag gctttgatcg ccgaattctc cgcccgcatg gttgtagacc
    48421 acatcgagca gcaccgccag accgtgaaga tgggccaggt ctaccagcgc cttgagctga
    48481 ttcatttccc cgtgtaagtc agccgcgtga taccggcgca gccctttcgc atccagcagc
    48541 ttgttcacct ctgccacata ggccggcagc tcagcatccg ccaccgcaaa gtccatttca
    48601 ggcgaaaaat agtcggtccc gttgtacccc agactgaagc tggtctgaaa ttcctgaatg
    48661 ggcatgagtt gcaacgcggt cacgccaaga tcggcaagat acggcagctt gcgcgcgaca
    48721 tcgagaaacg tgccgccctt gtgcggcagg ttcggcgtga aaaaggtgcc gacatggagt
    48781 tgatagatca cgaactcgtg aaacggcggg gtgacatagc cgctctcatg ccagggaaag
    48841 tcggtgcgcc gaatcaggca ttcggcggga aagggcgatt ccaattcacg ggcgtaggga
    48901 tcgcgcttga gcccctcgct cccctcgccg accacgtaga acatgtaccg ttgacgatcc
    48961 ttcaccccgg ggaagaaacc acgccaatgc ccctgctgat ctcgcgtgag gagtgctgcg
    49021 tcgttgcgga tgcgatgatt gaaatccccc accacgtaca cggccttggc atgcggagcc
    49081 cagcagcgga acgttgcgcc gccgcccacc agcgtagctc ccatgggagt atcgcgatca
    49141 atgtgtttga gcgaggcggg catgaatgcc ctccggggaa cgatcaggcc gacgaagtca
    49201 tcctaaagaa aggctatggt atcgagcaag ggggcgccta agagaccgag cgccgccgca
    49261 tgtagaccat tcaggcaacc ataccccata cagtcggggt caactctgac gatgggcacg
    49321 gatcagcctg acaatctgat ccgccgcagc gtcaggcaac agctccgtgg tattgagaac
    49381 caggtccggc gagaggggcg cttcgtaggg ttcctgcaat ccgggcacag tggaagattc
    49441 gccttgcagg ccccgccgat aggttccctt cttatcccgc tccatgcaga catgcaacgg
    49501 gcattcgacc gagacctcca acagatcagg aatcagcgtg cgggcaaact ctcgataggc
    49561 ccgacggctc gcagtcgcat cgatgatcac attcacccca tgcgccagca atcgcgctcc
    49621 catgaagccc aaggcccggt agaacagatc gcgttcctct ttcgaatagc tggcctccgg
    49681 cgtcaaaatg cggcggacgg catccgactc cagcacctcg gcggtcatat ccatcgaagc
    49741 aagcttgggc agcagcctgg ccacgataga acttttcccg gaagccggga ggccggtgag
    49801 ccatagggca aagggcgcca tcgtcatgtt caccatgtga gagccgcctc attgcacgag
    49861 gcgcgactgc gactttgact gtactaatcg cagtaccgat tcacatcggc cggatcgaag
    49921 cgcgggacgg ccagcacatt ctcaatgaag cggaatattt ttcgccgcag cgtcagggtc
    49981 aacttcggat accagagtgg actggccaac accaaccccc ggaaggcaaa aaacggcccc
    50041 gttgccgtta gtacgtcttg atcgtggctc ctgcttagat agcggtccca gaacgaatgg
    50101 aacaacacct ccagcggtcc tttcaacgtg ccatgccggc agagcgagaa aaatagataa
    50161 ttgatcgaca tcgacgtcac atcgtccgcc ggctcacccc actccccacg cgagcggtca
    50221 agcaccgaaa agtcggctcc ccggcggaac aacacgttcc agggatgaaa atccccatgc
    50281 acttgtgaga gacgcgcatg ttgacggcgc aatcgccagc gccaggcatt acaggcgact
    50341 tcgatgcgtt gcagcaactc ctccgtgatg aaaccatagc gatgcggata actgtcggtc
    50401 agtcccatga tacattcgcc gtggccgagc aattcgcgca agcggcggcg atagagttgc
    50461 ggatctctgc gcttcacgcg gtgaatggtc gccaaatagt cagccagcgc ctgtgtccgc
    50521 ttccggtcca acggggtagg cctggttgcg tccgccagtc gttcgagatc cttgtgatac
    50581 gtgtcccctt ccgaccattc ggtcagcagg aagaactctt tggccgccgc gaccgacatc
    50641 aactcgccac gcgcggtgaa cgcgcctacg tccagcgccg tgatatgccg gggaagccgg
    50701 ctgtaacagt catagtccat caacatcgcc tgggctctgt cagccggatg ctcgtggccg
    50761 aacggaccgg gactcatcgt acccagcacc acttggcggg ttttgttgcc gtttgtacga
    50821 aaggttaatc ggatcggcgc gccatacccg tactgtttgt atcgtgcacc ctgggtttct
    50881 tttccgatgg ggccataggc caccaattcg gccgtcgaac cgaaccgtgc tttgacgtag
    50941 ccgtccaggg cggatttctt caataccggc atatccggct cgtacgcaaa acatgttccc
    51001 gaaaaggagt agccacgccc gccacatcga gccgagactg gtgcattggg cttcaacccc
    51061 attcaggcaa gtgaggaatt atgcgccagt ggtatgcaga cctccgcccg gcgtagtaag
    51121 ccttcccatg aaaatgcctc actttgagcc gattcaccct tgcctcgacc gccatacagg
    51181 ctgattgtca ggggcatgtg ccttgctgag gagagcagtg agagtccctg ttccatctga
    51241 gacgagagga ggacgcatga acctggaagt ggaaagccgg aatatcgaaa tgactccccg
    51301 ctggaagacg gaaatcgaag aacgcatggc cgctttgcaa cgtggccatg acgacatcat
    51361 ccacggccgc gttacgctca ccaaaaatcg ccatcacaag aaactggaca atgtcgccga
    51421 agcgctcgtg ctcgtcacgg tgccctcgcg gcagaccatt acttcacgca aagaggataa
    51481 gaccttcgaa gaagcgatcc ggtccgcctt cgatgccgtg gcgatcgagt tgaaaaaatt
    51541 tcgcgagaaa cgcgcggaca aggtcattcg cgtggaaccg ttgccgcagc ggcgcggtgt
    51601 cgtgagcaag gtctttcctg aacaagggta cggcttcatt ctgatggacg gcggcggcga
    51661 ggtgtatttt cacaagaatg cggtcaaggg catggccttc gccgacttgg aagacggagt
    51721 ggaggtgttg ttcgaaagcg agcccggcga gaagggcttg cacgccacca tcgttcagcc
    51781 accgcatctc ctcaagcttt aggacgtaca cgatgctgga caaccataga ctcccggctt
    51841 ctctcctggc tccgacggtc gatccggccc gcttgggatt tgccgatacg agccaggtgc
    51901 agccgctcga ggagacgatc ggacaggagc gggcggtcga ggcattggaa ttcggcctgc
    51961 agatgaagag tcccgggttt aacatcttcg tgtcgggccc tgtaggaacc ggcaaaggga
    52021 cgctagtccg acagatggtt caacgcctgg ctcagaccgc tccgccccca ccggattggt
    52081 gttttgtcca taatttccag gacgcctcgc gcccgtcctg cctttcgttt tcagcagggc
    52141 aaggcgcgtc cttcaaacgc gatatgagca ccttcatcga aggtctccgc cgagacattc
    52201 ccacgacctt cgaagggaaa aagtatctcg atgccaaggc gaaaatcatc gaagagacgg
    52261 aagataaaaa gaagaatctc tttcatgatc tcacgatcct ctgccaccag agaggattcg
    52321 tgtttgagga aaccgcgggg gggttcgcgc tcgttcccat caaagacgac cgcccaatga
    52381 ctgggaagga aatggaggag ctccctgagt caatccagga agaactgacc gcgcggcggc
    52441 aatcgctgga gagcgacatc cgggagtttc atgtccggat gcacggcctc gaacgggaag
    52501 ccgaacaggc gttgcatcac ttggaccacc agatcgtggc caacgtcatg aaaggcgcct
    52561 atgacgctct cctgcaaacc tatcaatcct cgcagctcgt cgtcacgtat ctgcagcggg
    52621 tccatgacga tatcgtgcac cagtacagag acttcttgcc tcacactggt cccatgctgg
    52681 caattccagg cctggagcag tcacgacgtc ccgacctgac acgattcgcc gtgaatttga
    52741 tcgtctcccg ggaggctgcc ggcggcgcac ccgtgataga cgaatcacat ccgacctaca
    52801 gcaatctcat cggcaaaatc gagcgacgtg cccatcttgg cgtcatgtat accgacttta
    52861 ccgaaatccg gcccgggtcc atgttgcagg ctaatggtgg gtatctcatc ctgcaggcgt
    52921 tggatctcat gcggcagccc ttctcctggg aggccttaaa gcgcgtgatt aagacgggag
    52981 ccgtgaccat tgaggatccg tcggaatttt ttggattcgc cacggccggc ctgcgtccgg
    53041 agcccattcc cgtctcggtg aaagtcatcc ttgtcggcac cgccggcatc taccacctcc
    53101 tgcaggccta tgaggaagat ttttcaaaat tattcaaggt caaggcggac ttcgacgtgg
    53161 aagtgccgca tgatgagcac caggaacggc agtatgcgcg attcgtttca cgcttgtgcc
    53221 gggaggaagg gctgccccac ttcggcgcgg acgcggtcgc cgaagtgatc cgccagggat
    53281 ttcggttcgc cgaccgccac gaccgactct cgctgcgctt cagtctggtg agcgacttga
    53341 ttcgggaagc cggctactgg gcccaaaaga gcgggcacgt cttggtgacc cgcgcggacg
    53401 tcgaatccgc cctggcgcat caacggcgcc gggccgattt gcctgagcag tggctccagg
    53461 gagaaatcgc cgaaggcaca ttgatggtgg atctccaagg cgaggtcatc gggcaggtca
    53521 atgggttgtc cgtctatgag ctgggcgatt actgcttcgg ccggccgacg agaatcaccg
    53581 cgcgcaccta cgtcggcacg aagggcgtga ttgatattca gcgggaggtc gatctggcgg
    53641 gtgagatcca cagcaaaggc gtcatgacgc tggccggttt cctggccggg aaattcgccg
    53701 gcatgcagcc atttgccttg agcgccacct tgaccttcga acaaacctat tctgaagtcg
    53761 aaggagacag cgccgccgtg gccgaactgg cagccgtgct ttcaagtctg gccgacctgc
    53821 ctgtccggca gtatctggcc gtgacaggtt ccgtgaacca gctgggagaa atccagccca
    53881 tcggcggcgt gaacgaaaaa atcgaagggt tttttgaatc gtgcaagcgg cggggcctca
    53941 caggcaagca gggtgtgatc attcccgccc gtaacatcaa acacctggcg ctgaatcggg
    54001 aggtcgtcgc ggcagtggag gacggacggt ttgccgtcta cggcgtcgat acggcagacc
    54061 aggcgctgga gttgctgatc ggcgtgccgg caggggaacg cggcgcagaa ggcacctacc
    54121 cgccggtaag catttatggc cgaaccgccg cacgtctcga agagatggcc cagatcgttg
    54181 caacctgggg tgaggccgaa gaggacaaca gggtcaagga ttagcaaaag cagtatcacc
    54241 ttgcgagcga ggcgcccatg ccgatgtatg attacacctg tttggattgc gggaaagaat
    54301 cgctgatcgt cgtcacgctg aaacagcacg aaaaaggcga ggtacaatgc ccggcgtgcg
    54361 gcagcaccaa attgcagcag catttttcct cgttcatcgc acatacgacg aagaagagtt
    54421 aaaggagacg cagcgatcca tgcggacgtt cacaaccctc agcattcgtc acctgattct
    54481 cgccactgca gcaggcctga tgctggcgcc agtggtcgac gacgcgaacg cccaggaatt
    54541 tcctccggat gccgccaaag gcaaggcggt gtacgagcgg cattgtctcg catgtcacgg
    54601 cgccggcggc cgcggagatg gccccgatgc tgccggattg acagtgccac cggccaattt
    54661 ccaccgcttc aaatccttcc tgaaatccga cgaagaactg cttcggacga tcgagcacgg
    54721 cgttgtcttc agtccaatgc atgcgtggca agggcaattg accgaaaccg agcggcagga
    54781 tgctctcgcc tacatccgtc tgctcgtcca gcagggaaaa tgacgcagcg ccccgccgct
    54841 gcgggccgat gtcgtatgga cgggacctgc tgcatgacga acctcacctc tcatgacgcc
    54901 ttagtcgtgg tggacgtgca acgggatttc cttccgggcg gcgcattggg catacaggat
    54961 ggcgacaaga tcgtcccgat tctggctggg tacgtggcac aattccacgc tcgaggcctg
    55021 ccgattttca tcactcgcga ctggcatccg cccgaccact gttcgtttcg agaccaaggc
    55081 gggccttggc ccgtccactg tatcgcagga tcgcccggct ccttgccgcc accgaacttc
    55141 cagcctcccg tctcggcggt cacaatctat aaggcgatcg atcaggacaa agaagcctat
    55201 tctacattgc agggcacacc gcttgatcgc catttacgcg ccctgaaggt ccgccgcctg
    55261 ttcatcggag ggttggcgac cgattactgt gtgctgaaca cggtgaagga tgcgctgggg
    55321 cttggttacg atgtgtgcgt actcatggat gggatcaagg ccgtcaatct ccaccccgac
    55381 gacgggcgca acgctgagga agccatgttg catctcggcg ccgtgcccgt ccgcctcgaa
    55441 agcctggccg catgacacct tcgaccacct ccctgctcac cgatctctat gaactcacca
    55501 tgacgcaggc ctacctcgct cataacatga cgggcgaagc ggtcttcgaa ttttttgtcc
    55561 gcaagttgcc gacccgcagg aatttcttca tggcggccgg actggagcag gtcctctcct
    55621 ttctggagca gttccatttc tccgagggcg acctcgcatg gctcgaggag aggggccgtt
    55681 tcccgccaga gaccctcatc gccttccgag cgatgcgctt taccggcgat gtccatgcca
    55741 tgccggaagg cacggtgtgt ttcccgcacg aacccatcct tcgcatcacc gcgccgctgc
    55801 cgcaagcgca acttgtcgaa accagggtga tgaatctcct gaatttccaa accatggtcg
    55861 cctctaaagc ctgccgctgc atccttgcag cctccgggaa gccgctgatc gatttcggcc
    55921 tgcgccggtc ccatggtgct gaggcgggac tattggccgc ccgggccagt tatctggccg
    55981 gatttgccgg cacgtcgaat gtcctggccg gagcgcagtt cgccatcccc acctacggga
    56041 cgatggccca ttcttttgtg caagcccacc gcgacgaaac cacggcgttc ttacatattg
    56101 cccagtcaca accgcaccat gtggtcctgc tcatcgacac ctacgatacg gaagcggcgg
    56161 cagaacgcgt cgtcatgctc gcccctgaac tcaaacgcca gggcattgcg atccatgggg
    56221 tacgtctcga cagtggagac ttgggcgctc atgcgcgcaa ggtccggcac atcctcgatc
    56281 gcggagggct ggaggagacc acgatcctgg ccagtggaaa tctggatgaa gaacggatcg
    56341 aggccttggt gacaagcggg gcgccgatcg atcgctttgc agtcgggacg gccatgacca
    56401 cctcggccga cgcgccctac ctggactgcg cctataaact ccaggaatat gccgggagac
    56461 catgccggaa gcgttcagag ggtaaagcca cctggcccgg ccggaaacag gtctatcgac
    56521 ggctgggacc agatggacga ctagcacagg atacaatcac cgtcgaaggg gacactctgc
    56581 cgggccggcc tctcctgcgg caagtcatgg agggagggcg gcgactggct ccgagtccct
    56641 cgatcctgga aattcgcgac cggacacgat tggctttgtc ggaacttccg gatacgctag
    56701 gccagttgga tcaggctgag ccttacgagg ttcggatttc cggcgccctg gaacgcctgg
    56761 cgcagcaggt cgaccgatac acatgactcc atgcgaacaa ccagccgctc cagcgaggag
    56821 cggcgtccac gcttgtgaag gaggcccttt gatgaatcag cattccttgt ccgcggcatt
    56881 cgttgccgga ttggtggtga ccacgctctg ttccattcca gacggcgcgc tggccgcagc
    56941 aaagtccgca tcgaaatccg cgctggccgt cgcatgcgag agccagtatc agcgcacccg
    57001 tcccgcgcca aaagtcgtgg acaaagtgtt gcaggcccat gcccgctggc tcgaagaccg
    57061 ggaatctgcc gatggtcgcc gggcgaatct ctgtcgcacg gacctgcgac aactgcggct
    57121 gacgacggct tcactcgaac gggcgaatct cgaaggggca atcctgaagg gcgcgaatct
    57181 gcggagcgcc aacctggttc aagcccatct gaaaggggca gacctgtctc acgccattct
    57241 cgacgatgcc aatcttgagg gggcggacct gcgcaaaggc ctgctggcca aggctcatct
    57301 caatcgcatc tccgcggacg aggcggcctt ttacggggcc aacctccagg gcgcattctt
    57361 tcgtgaagcg ctgttggagc gagcgcattt tgaggatgca gatttacaac tcgcagacct
    57421 gagcggagcc agcctgctcg atggatattt ctatggcgcg aacctctcaa aggctaatct
    57481 aacggacgcg gacctagccg gaaccgacct gcgccgaacc aatctccggc aggccactct
    57541 gcgacgggcc aacctccaag gcgcgctcct ggacagcgcc acactcgacg gcacctcact
    57601 ggtcgaggcg gatttggaaa gcgcctatct ggatgatgcc tcgctggtcc aagcggatct
    57661 tcacgaggcc agcctgagag gggcagattt tcgatacgcg cggctgacca acgccaacct
    57721 tcaacgggcg aatctcgaaa acgccaacat cgaaggcgcc aatctggcca aggctcgctt
    57781 gaattccgcc acgatgacca tgtcggtgtt atacaaggcc aacctgacgt cagcgaacct
    57841 ccatggcgcc cgattgcatc atgctgtcct tatcgacgcg cacctgtcac gagccgacct
    57901 gcagaaagcg gatctaaccg aagtctatgc gcccaaagcc catttgcagc aagcgcggct
    57961 cgccgaggcg aatcttgaac tggccaatct ggtatccgct gacttgagcg aagccgacat
    58021 gagtcactcg atcatggtcc agaccaattt gcaggaagcc aatctgcgag gaaccaactt
    58081 gaccgccgcc gatttgactg gagcccaact caataacgcc gaccttcagc aagccaactt
    58141 ccgcggtgcg aacttgagcg gcgcgcttgg actcgtgcag gcacaactag accaggcctg
    58201 tctggatgag gccacccaaa tccctacaga ccttcagcga ccgaaggcct gcccgccgcc
    58261 acgtcagaag cgtaagtagg gaactgccca tcggaatgtc ggggcaaaca tccgggctat
    58321 cgggaggaac actcggacaa gggctaccat ggcaagggcg gggcagagat ggattgatgt
    58381 agttgaatga agtgttagtg cgattcagga caggtcacgc ctcccttgcc ggtgggctct
    58441 cgggagatca ccgtgaacct tcggcgcaag acgatgttcc tgctggccgg gctggcggca
    58501 ctggccgtga cgctcctcct catctggtgt ccgcctgccc tctgtcgggt cctgaacatt
    58561 tatacctttc ccctctccaa cctgagcgac tttcggcaat atgtggcaga tgaagaggcg
    58621 ttctactcac tgcatcacac ccgcgctgtc cgtattgatt gtgcagccct gcgcgatgag
    58681 gtcgtgacgg tcctcaagat cggacatctg gtgttagaaa ccaacacacc cggcgacact
    58741 caacgtgaac gtagaccagg cacaatcgag gcctggcatc aggggcgtct gttacacctc
    58801 acgaatacga ctttggcgtc gaaagatgaa caacaggcct gggccacctc ggtgcgggca
    58861 gtcgtcacgc tgcaacaaca gcaagacctc gcatttcttt cccgactgtt tggcgctgtc
    58921 gtcctgcatg tgtctcacag gcagataccc atcgtgctgt tctccgatcg tccctgtctc
    58981 tagcaactcc acattgtcgt cgtattcccc gcgccgcaga ctctgtactg gtgacggcca
    59041 gaacgccgcc gtcacacctg aatcgacata acgcgttgag aacacacgtg ctgaagcact
    59101 cttgttccct atttcctact tagggttgcg aaccggctga actaggcaca tttccagggt
    59161 gccggacacg gaagcagcgc aaag
//
LOCUS       DAOQDS010000018        57384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_169_length_57384_cov_17.365993, whole genome shotgun sequence.
ACCESSION   DAOQDS010000018 DAOQDS010000000
VERSION     DAOQDS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 57384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57384
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169_length_57384_cov_17.365993"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PK589_06180"
     CDS             complement(<1..191)
                     /locus_tag="PK589_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19221.1"
                     /translation="MTVSGGFTQTIDWLAFTLPKAEVADVIALIGGDWFQSETGFRGY
                     PVAQLMTEGKTGVGKLGTGA"
     gene            complement(188..499)
                     /locus_tag="PK589_06185"
     CDS             complement(188..499)
                     /locus_tag="PK589_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ19222.1"
                     /translation="MEQTWLTVDELAAVLKVSPKSIRRAYRKGEIPVDRFCRFVRFDL
                     ERVKEALQAKGHRSSLLLPQNKEGQRSATGGDSRRRAQPTRPRLGKTGASIAQKPRGK
                     K"
     gene            1268..4543
                     /locus_tag="PK589_06190"
     CDS             1268..4543
                     /locus_tag="PK589_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNJ19223.1"
                     /translation="MELIDNISRLLGDDLKQVIRPGSRLKVAASCFSIYAFEALKAEL
                     EKIESLQFIFTSPTFVPGEVTDKFRKEHREFHIPKLERERSLYGSEFEVRLRNELTQK
                     AIAKECAEWMRQKASFRSNRGLAPMQQFAAVTSSGKESIYLPLHGFTAVDLGYEKGNA
                     ISNFVNRIDEQPLTTTYLSLFDQIWNDPEKLEDVTARLCEHIASVYQENSPQRIYFLM
                     LYNIFSEFLEDISADVLPNDRTGYQDSLVWKKLFNFQRDAATGVINKLETYNGCILAD
                     SVGLGKTFTALAVIKYYELRNRSVLVLCPKKLADNWLTYKGNLVTNIFAKDRFGYDVL
                     CHTDLLRESGYSFGLALNRVNWGNYDLVVIDESHNFRNNTAFEDRETRYQRLLNQVIR
                     QGVKTKVLMLSATPVNNRFTDLRNQLALAYEGEPEILSTKLKSQKHIDEIFRRAQAVF
                     NAWSTLPPEERTAAAILNTLDFDFFEVLDSVTIARSRRHIQTYYDTKDIGTFPERLKP
                     LSFHCQLTHRKDVIGFNEIFARLNRLTLAVYAPLSYVQPSRLSQYEKLYDPDAAIGHG
                     NLKQTGREKGIQALMTVNLLKRLESSVHSFRLTLKSLAGHHRHALEKIAAFKKTGRNA
                     TFVDVSPAFAEAEPDEESEFPNPDDAQIGKNVQINLSDMDLPRWEDELRADLVIIEEL
                     LAEMHKVTPSDDAKLQHLKTTILSKLASPINVGNKKVLLFTAFADTADYLYEHLAPFF
                     WEKKQLHTGKVTGANSPKSTLKKTYDFQSLLTLFSPLSKEKAAILPKEPGEIDLLIGT
                     DCISEGQNLQDCDYLINYDIHWNPVRIIQRFGRIDRIGSQNKCIQLVNYWPDITLDEY
                     INLKERVENRMVIADVTATGDDNPLDSRANDVAYRREQLRRLQEEVIELEDLKTGISI
                     TDLGLNDFRMDLVSYVKAHGDLASLPNGMHAVVPADPERGLVPGVIFALRNRNEGVNI
                     NRQNRLHPYYLVYVAQDGNVVVHHTDAKRLLDLARAACKDRSESIPEVYQSFNRATQD
                     GRRMDAYSALLQKGIQSMIAQKEEKDIDSLFTGRKTTALLNTINGLGDFELIAFLVIQ
                     SRA"
     gene            4550..5206
                     /locus_tag="PK589_06195"
     CDS             4550..5206
                     /locus_tag="PK589_06195"
                     /inference="COORDINATES: protein motif:HMM:NF025693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4391 domain-containing protein"
                     /protein_id="HNJ19224.1"
                     /translation="MFAYPKQAEFNRLVPKSKIYTYGRVSKRIKDLFVAQVGEILWKY
                     KLSPETINLPARNGINEIQVFDIALKKPELDTDILQAIDKVIPFPLVFELTYDGQVHF
                     AASYKRPSEADPSKWVIEATFQTDPQPVNAERKPLPVALDLASLYEQIVGCHVPLPSR
                     QGEGIAEHVARFNEVEAKKKMRQQLESRLAQEKQFNRKVELNASLRSLSQELDSLTRH
                     "
     gene            5218..7095
                     /locus_tag="PK589_06200"
     CDS             5218..7095
                     /locus_tag="PK589_06200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNJ19225.1"
                     /translation="MDKLKMHTPNLTAEKIEKVAALFPNCMTETKDEKTGQLKRAIDF
                     DQLRQELSDHIVDGPRERYHLDWPGKREALLAANAPIAKTLRPCREESVDFDTTKNLF
                     IEGDNLDALKLLQETYLGKVKLIYIDPPYNTGNDFIYEDDFAADSDEYQLRSNQKDAV
                     GNRMVANTESNGRFHSDWLTMMYSRLKLAFNLLRPDGAIFISIDDHEVHNLRRVCDEV
                     FGDTNFVSDIAWQKRYTRSNNTVEFTTVIEHILVYRRSPEFCVNLLPRTDDADARYTN
                     PDNDPRGPWKGASFLNPATPQERPNLCYPIQNPNTQSVTHPTTNAWRRSRDEFERLAQ
                     EKKLYWGLDGTSPIPSVKMFLSEARGLTPINFWSHEYAGNTDQGTQDLEMLMGGKYFN
                     NPKPVVLIKRILEHATGPNDLILDFFAGSGTTAQAVLQINSEDSGVRRFILVQLPEAA
                     DPQSAACRAGYTTISDLAKERIRRAGSKVSTDNGLISSKLDVGFRVLKIDTSNMNDVY
                     FVPDSVRQEDLLAHTDNIKGDRTPEDLLFQVLVDWGVDLSMLIEKKTIGGSAVFFVDG
                     NALAACFDMDIGEDLVKELAERKPLRAVFRDSSYGSDSLKINVEQIFKLLTPATEVRS
                     I"
     gene            7133..8203
                     /locus_tag="PK589_06205"
     CDS             7133..8203
                     /locus_tag="PK589_06205"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HNJ19226.1"
                     /translation="MPISSRISGIDGPPLGQILRTELINGEDRALGIASAFVSVGGLR
                     DILAITKKCTSLECRLLAGISNAVTHPKALTEALDAGWKVRLGTALGARIFHPKMILS
                     GRVFLRDGRVKEPSFAYVGSSNLTKGGLYKNIECGIIAHADFAPPSISLCFARLWKEG
                     KAATQESIESYAEEFARRNRKRSLDDLVELGVVDNAEDQKSTYKNLALQRDTPRQGVI
                     SEGVAGAAWAGLESFTGDYQFQVEFPQAAGIVLNRIIGGSSSSKVPILCTTDNTVREM
                     TYRYYTDNGMFRLNIPNDTPGVHRARKSHKGIALIEVSERPDVIAQLTIVPPGRTLEQ
                     IIRRSTLLGTWGSTSTRNYGWY"
     gene            8278..11304
                     /locus_tag="PK589_06210"
     CDS             8278..11304
                     /locus_tag="PK589_06210"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNJ19227.1"
                     /translation="MKLKFKQQGYQTDAVKAVVDCFAGQPKSRGSKYAIDPGRAAVGG
                     TVGLPMESEGFANAGLALSPVQVLENLQEVQRRQNLPMSSELKKTTVCDLNLDVEMET
                     GTGKTYCYIKTMFELNREHGWSKFIVVVPSIAIREGVLKSFEIMAEHFLEQYGRRARY
                     FVYNSKQLHNLESFSSDAGINVMIINVQAFNARGEDARRIYVELDDFQSRKPIDVIKK
                     NRPILILDEPQKMEGKATTEKLAEFNPLMILRYSATHKTEHNKVYRLDAIDAYNQKLV
                     KKIAVRGVTVKGLAGTNAYLYLETIRIATTKPPEARAELEILQANGIKRVHRLLKKGD
                     DIFTLSDGLEQYRGFVVSDINAIENTISFTNGVVLGAGEATGDVNESALRRIQIREAI
                     KAHFEKEKVLFPQGIKVLSLFFIDEVAKYRSYDGAGEHAGEYAKMFEEEYDAQLNELP
                     LLEERPYNRYLKGIQTSKTHDGYFSIDKKTKRLVNPDVKTRGETAGEADDVDAYDLIL
                     RDKQRLLTFEEPVRFIFSHSALREGWDNPNVFVICTLKHSDNTVSRRQEVGRGMRLSV
                     TQNGDRMDDPAIVHQVNVLTVVANESYKDFVAGLQKDISASLSARPRQANEEYFRDKV
                     LKAATGDVKVTPHMAKLIERYLVKNEYSDTNESITERYHKAKKEGALATLPAELEPYK
                     EQIFQLIDSVFSAAQLPEIEDDRKGKDNHRNANFDKKEFQELWSRINRKAIYAVDFKT
                     SELIEKCIKALDKEIRVTPLHYTVTSGIQKDEADYDEVKKGDAFVISENLTDNVRSSA
                     RSSVKYDLIGKLAEATQLTRQTIATILRGINLAVFGQYKTNPEDFILKAGTLINEQKA
                     TVIIEHLTYNPLEETHTIDIFAQQKKEDISKGFKANRHIYDYVFTDSGNERTFVGELD
                     ASSEVVVYAKLPKVFYIPTPVGNYNPDWAIAFEAGKVKHVYFVAETKGSMSSMDLRKI
                     EESKIECARKFFAKITSDQVKYDVVDSYGKLMEIVK"
     gene            complement(11756..13051)
                     /locus_tag="PK589_06215"
     CDS             complement(11756..13051)
                     /locus_tag="PK589_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19228.1"
                     /translation="MSDNDLISSYSTDKLLSYYADKVKQPAWLRWFRNADIALDKSKE
                     SLPSQAGKFVRLGREITNEMRYRSAMWKWLHPANPTLCLDELSSLQKLTAFDHLDSKT
                     FLRGLRDHCREFMRQHPQLRLWIEKRNALLDFAIEQACDIPEQLSLTAIALRQAQNQT
                     YGLISDGQSAQAIDMAGQISERAMYAAVQAVLNVLGKNKTDACPASANLMVPCRVADL
                     ECDGFLPNELARQNHARAARLWKHLAQPNKCLVVIAETAEAGHLGFWVPLDSGGSRLS
                     LAGAPTAFIHQEGTAVFKDDLPLLQGVPDALAVEWTAYMKSEFQEQMFVSLPFIVPKT
                     DTVGGKLVAGVLNVNASPPSDDGWRRAYHKEWLALARKNIDQFVEVAFIVNKLKSKAR
                     TNQDLKIDTGSSLWDSLPYALIPPPAPKMLSLPKKEGDQ"
     gene            14672..17914
                     /locus_tag="PK589_06220"
     CDS             14672..17914
                     /locus_tag="PK589_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19229.1"
                     /translation="MADAKESDLTGCTEEVPLLGVTREFDFGQFIELYDDSSDPNDPK
                     NTPTISRIVIQPNSELAAGRNAVTGEPPPIKVSSSAISVASTVNTPQNNGHQVPFILE
                     TSGGASSRSAKEPEVHLFDLAQYGPAIKNGPIDVQIVDADRFLENGTFEMRGRTYKVN
                     LEEASMDAIVKGMAVTTIASSGKASSSTVPVRLFLSSEPVNMTGGQLAKVLSGQPVES
                     SIGEVLPALTTSAIRTLLSGGTVLTHGVASDGRKRPLTLHVPDSNAEPLTDHFEVFDV
                     ASFLTSPTVMSKGGKHLPLKLMPEQVNQLISSGHTRLELAGRSIDFTVVKDHQESSIR
                     GVSSFKEDLKKKTLPQGVRIPGMSLDEMVAYGKQFEKYEFVHPYQPNKYNGIASEAPS
                     DGSASGAGLHSMTEKVQPRLPSGSGLPVAVFVPWKQTWMLKGFSRGNLLQTIAMAPQE
                     QITMQVLSWERRSRSLDQSSETEVDQQTDVTQTTRDTEDVFKEMISKHDFAWQISGSI
                     DASYSPGVASIQVHSGGGVTDTSNIVQTARRSSQSVKESTVKASSRVRSRRVTRITQT
                     VESGREERVTRVIRNPNQCHTLTLDFFETLAHYEIKLEFLKDRLRLVVLVPNPINVPD
                     FLSEIIRRNETALRNALIDTALVDGFEASRLVAAYEEAKMLLALQISEAAKVNEVETQ
                     RDKPSPSGSPDPAAPQQTELERVVGEMISILKNMKDAEIDTALLAIAAHAGVPETARR
                     KGQQWLFINFCNAKLPALLSTLDDITKGNIAPVVAAQKMVAVLPKPDAPTNLGNLNQM
                     SNAEKETACITSKLREETNQERLYMKRDWDWGWWSGRMQEEGLYTANDGGLAGLADQL
                     QKAYAAWEAKKAQGDAMKDQEVAKTEAEGKQEKASTDDKLAMAFPLEDLARAYERMKT
                     LMSHLNEHRAFYNYALFQALPPSEQTVRIVEASNGRLQVGLFEPRVVAMSGNKLVVPL
                     TPLANTTQLQTFVTSLANDLENAFKTTLTTPDTTVLPTPGVSVSSRLGKCSACEDYIE
                     KAREHELKRLEGLARQEQAEADRRKARLEKNDFDDFRQAPPAIKLDLDKT"
     gene            17920..19563
                     /locus_tag="PK589_06225"
     CDS             17920..19563
                     /locus_tag="PK589_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19230.1"
                     /translation="MADPNNPSPHTVRLALAIPILQADLEDEEVEGSSPAPAGSEPPT
                     DPYMALAAREKAWAMVAIWPDQDSVRIEIPYAETDALYELIETLYLIGGWAAVDKVCE
                     STSITLVGAVSQILGVPRGENFAITWLLSDGLLRIPPEHLDLAKKAFLVLLARAADRS
                     WPSVMTGLQAIVPFSGIFSYGPSHTGPYPSDVPNPEVIARLYANVVVNLEFVRRQLTT
                     RIRNTLHRTEQNAKRLLSQSLLNSRADIIRETIRYFDFEQGSIDHVFKSTKRKWVKLS
                     SEPGNMTRPAEQPAALQLALKKLVPFAQRINKLKGSARGAGTSTQKMVSEALQNARSI
                     FAREMGRLAQEFPILSQIEENHIKDASEAYAPQLGSILFSILERANDANHETTLRLDG
                     FGDLIITLTEGRHPEQDLAEAVQRLGKRNTIWGITKYVERAISQATSVTDEVAQKALA
                     DTVMLLGIESGETVKALAQGAIEMAALGMASNFATRFVPVLNIGLAAWHIGKSVKEFS
                     EQEADFYCVLDPRDSLVSAAPSVAGLTIDIATEAVFAII"
     gene            19938..21110
                     /locus_tag="PK589_06230"
     CDS             19938..21110
                     /locus_tag="PK589_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19231.1"
                     /translation="MFDRQLKEFSVIKGEILRDIEKSVDAVAKRLQAQTVGQRATKVR
                     KDVQTALDEAEQQLQNQRKVLTGKIEDSSSAITHQKRAEAHVTMVMATKDTGARAFIN
                     SRIKRLMQFPKGREMAADFGKELKNKVRHFLPILDEVEDSAPFFMEALGIVASANGKG
                     TRLLRSWAQGKTKIDSVSGYLAHMKGILPEEVVTRMKFLESIFHKNAFEILSEFPPKL
                     RGQMSVEIVEGPLWVIDHSGAPRQFGDSCMLLTGPNGQAAIIGLAEVKAGFDEDLLKQ
                     LFARSDARALNSSVTFIDRNGKSQVRKLTREFSFEGGEAVPINKPPTYVYGRTKGEKK
                     ETAEEFQKLVNDQMRRDREVWKVPLPLSLQQNQRFSEQALREAVKAIVKADPNWGL"
     gene            22390..23430
                     /locus_tag="PK589_06235"
     CDS             22390..23430
                     /locus_tag="PK589_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ19232.1"
                     /translation="MVDVSCLICGGVYHETVAFAAEIAEQKELLRRFHQQRQRQDAQA
                     DLKDRTTFSQDYSTEVVRCLTCGFVYRNPRPTLRSVTAQYADDQYGEEHLEAEFQAQQ
                     RWAEWKITAFAQHCHFTSRHPVVVEVGSFVGGFLAAAQQRGWSILGVDPGKEVVNFCQ
                     ARGLPVFHGTLNDATIAARSVDAVVIWNTFDQLVNPDTTLSAARRLLREDGHLVLRVP
                     NGAMFQQGVHWLKAGGSRRRWAIVTLAWNNLLSFPYLNGYSVSTLDQLVARHGFSRVA
                     VEVDTLMTLSDQHTTLWGQWEERVVKSSCRILAGTTTLLPSLHNMVSPWLDVYYAVSG
                     DAATSPPIACPA"
     gene            23526..25874
                     /locus_tag="PK589_06240"
     CDS             23526..25874
                     /locus_tag="PK589_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HNJ19233.1"
                     /translation="MTITRNSIEDIWGPRTPYRGQWPERSDCNVEGTPEKWVPSACVL
                     CSNGCGVEIGVREGKIVGIRGRSDDRVNRGRLGPKGLYGWKANASPDRLTHPLIQKKG
                     RFQEVSWDEAMEVIVRKSQEVRQQYTAGAIGFYTSGQLFLEEYYTLGVIGKAGLGTPH
                     MDGNTRLCTATSSAALKETFGCDGQPGSYNDIDTTDALFNFGHNVAEQQTVLWMRMLD
                     RLCGANPPKLVVVDPRPVPAALKADVHLAPKPGTNVAIMNGLLHLLIKHGWVDYSYLS
                     AHTMGFEQLEKVVAAWTPARVAQVADIPERGLREAAEILGTTPTLVSTVLQGVYQSMQ
                     ATAAACQVNNLHLLRGLLGRDGCGIYQMNGQPTAQNTRECGADGDLPGFRNWDNPSHI
                     ADLARLWNVEPHIIPHWAPPTHAMQIFRYCEQGSIKFLWISGTNPAVSLPDLRRTRRI
                     LGQKDLFVVVQDAFMTETASRADVILPAAIWGEKTGCFTNVDRTVHLSRKAIEPPGEA
                     RPDLEIFLDYAERMNFRDKDDQPLIKWHTAEEAFEAWKACSRGRPCDYSGMTYERLSS
                     RSGIQWPCNSENPDGTSHLYVDGHFPSDAEYCETYGHDFDSGAAATPERYRAHNPQGR
                     AVIKASDYLAPFEEPDERFPLWLTTGRVVYQFHTRTKTGRVEELQAKAPEPFVEISSQ
                     DAADHGIADGERVEVRSRRGYVQMRAKITGIRPGVLFIPFHYGYWDENSHPRAANELT
                     LVEWDPVSKQPHFKCAAVRIGKPGKSGLVPKLVKTFVRKTFG"
     gene            25871..26326
                     /locus_tag="PK589_06245"
     CDS             25871..26326
                     /locus_tag="PK589_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19234.1"
                     /translation="MNIGVYVRLAHHSETQLAEAFLQVSDHHRAEPDVQETCRLLSSW
                     SKEKSNGIRPFIATYGEPRVNEPETLFSDLFQGPRTGGLALLRDLHDLWLLATEAMIS
                     WTVLLQASRALRDEDLEHLCTRHITQTDRQLAWLQTRTKQAAPQALVVA"
     gene            26512..27087
                     /locus_tag="PK589_06250"
     CDS             26512..27087
                     /locus_tag="PK589_06250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNJ19235.1"
                     /translation="MALLIIDMINPFTFPRAEDLFPAAYDVAVRICRLKQKANQAAMP
                     VIYINDNFGTWRHDFQTLVRQCRDEPCRGRGIAELLAPSQNDYFVLKPKHSAFFATPL
                     EILLQSLSVRRLILTGVTGDQCILYTAADAYMRGYGLFVPSDCTASLSAQVNEQALEH
                     MRTYFDANTCLSEQLITTAPAEALAKCGTPQ"
     gene            27146..27727
                     /locus_tag="PK589_06255"
     CDS             27146..27727
                     /locus_tag="PK589_06255"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNJ19236.1"
                     /translation="MNRDAKGWDVIIVGSGPAGLSAALYLGRSRRRTLLLHSGHSMAK
                     WEVNVQNYLGFPQGIAGTDLLERGLTQAMQFGVEVIEDTVVSLSVGDPFQLEGGKTRY
                     EAGRILLATGLTHVPPDIPGVKECLGHSLFFCKDCDAYRVQGQRIVIIGWNNEAAEYA
                     LGMLTFSSNVKVCTNGRPAAWIRPMPRGSTSMA"
     gene            27724..28119
                     /locus_tag="PK589_06260"
     CDS             27724..28119
                     /locus_tag="PK589_06260"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ19237.1"
                     /translation="MSISSSRIRTVHHESGFIRSFTLESSEQFEADAVFTTRGDVVHN
                     RLAAQAGAALNEEGQVVVDHCMQTSVPGLYAAGCLTPANCQMIIAAGQGATAGQAINR
                     DLFEESLRRHALPMRASTRDCRESHRLMC"
     gene            28135..28611
                     /locus_tag="PK589_06265"
     CDS             28135..28611
                     /locus_tag="PK589_06265"
                     /inference="COORDINATES: protein motif:HMM:NF039409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HNJ19238.1"
                     /translation="MAMLKSHSAPNLPQPEAASEVASHLLEECRMVLPGIQALFGFQL
                     IAVFNNKFGEMPEGHQTLHLLAIVFITVSIAFVMTPAAYHRQAHQFAISRTFVQISSL
                     LLLLSMILLSVGICLDVYVVCALITGSDAISLMVALAAALLFALLWFVFPRCIRNP"
     gene            28766..29272
                     /locus_tag="PK589_06270"
     CDS             28766..29272
                     /locus_tag="PK589_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNJ19239.1"
                     /translation="MAGKTAFASGVVEMLKEDHAKVKGLFEEFESADGQEQAEIAATA
                     IFELEIHAELEEKLIYPAIREAIDDSDMMNEAVEEHHLVHVLIKELKKLKPKDEKFHA
                     KFSVLSELVKHHIEEEEGEMLPEAERHDIDWDALEVAVMKRKEVLMAKSSKKSGSRSK
                     ATPKSGRS"
     gene            29394..29735
                     /locus_tag="PK589_06275"
     CDS             29394..29735
                     /locus_tag="PK589_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19240.1"
                     /translation="MLPCHPAHNIQAAARRRLSLKEKRGLMAGLAAKARDKLGRLRLG
                     RARQVQGELKAVKEAQGEGHLPQGPGWGRGPAWEVGQEWAPEAGEARVAAVLEVAAGG
                     NAQRWATEGQN"
     gene            29835..30269
                     /locus_tag="PK589_06280"
     CDS             29835..30269
                     /locus_tag="PK589_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ19241.1"
                     /translation="MPLLKEIMTPSVEVIAPNATAADAARKMRDLDVGAIPVCDGEKL
                     LGMVTDRDLVLRVLALSRSPVDALVSDAMTPGLVYCFEDQDAEVAAELMAEKQIRRIP
                     ILSKNKQLVGIVSLGDLAIDGLDQHTSGEVIHDVSDPSRGGH"
     gene            30321..30503
                     /locus_tag="PK589_06285"
     CDS             30321..30503
                     /locus_tag="PK589_06285"
                     /inference="COORDINATES: protein motif:HMM:NF040444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HNJ19242.1"
                     /translation="MARYSKGSQKTVQEAMHKRKKGTLKSGSGKKVKSKKQAIAIGLS
                     EARKKGKKVPAKKSAK"
     gene            complement(30522..31688)
                     /locus_tag="PK589_06290"
     CDS             complement(30522..31688)
                     /locus_tag="PK589_06290"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HNJ19243.1"
                     /translation="MKANCWYGAGDVRVEQVPDPTILNRRDAILKVTSTAICGSDLHL
                     YNGLMPTMKAGDILGHEFMGEVVEVGSEVTNLRVGDRVVTAFPIACGRCLPCRQELFS
                     LCENSNPNAWMAEKLLGHSPSGIFGYSHMLGGFAGGQAEYARVPFADVGTIKIPSDIP
                     DEQVLFLSDIFPTGYMAAENCQIQSGDTIAVWGCGPVGQFAIRSAFLLGAERVIAIDR
                     FPDRLQMARDGGADTMNYEEEEVYDRLMEMTGGRGPDACIDAVGMEAHGPGVLAAYDR
                     MKQAVMLESDRPYALRQAIMSCKNGGTVSIIGVYSGFIDKFPIGSIMNRSLTIKTGQA
                     HVQRYMAPLLERIQRGEIDPSVIITHRMRLDEAPQAFAKFNRKEDHCMKIVLTP"
     gene            complement(31739..>32506)
                     /locus_tag="PK589_06295"
     CDS             complement(31739..>32506)
                     /locus_tag="PK589_06295"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNJ19244.1"
                     /translation="GASGLREITMGLGILSERRPAGWMWSRVGGDALDLALLATALSS
                     AQGNRKRTAAAMLAVAGIAAVDCFASRHLSRMVGWTSDDGAVLIKKTIMIDSSPEALY
                     LFWRDFQNLSRFTEHVESVTIYDRHHSHWTVKGPAGYRVEWNAEITDDEPNRKIAWRT
                     TSQADIHHTGSVEFTPSPGGRGTLVRVVMEYNVPAGVLGASIAKLFGSEPGQQVQSDL
                     RRLKQIIEVGEVLRSDGSLHGKGILQQRPAQPAGSHT"
     assembly_gap    32507..32606
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32607..33999
                     /locus_tag="PK589_06300"
     CDS             <32607..33999
                     /locus_tag="PK589_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNJ19245.1"
                     /translation="VFLVSSQKRSSRFMDVALQYDRLQFAVTGTFHYLFPQLTMGLAL
                     FVLYFRTRDLFSVGEHFHEVARFWTGIFALSFAFGVVTGIPLEFQFGTNWAAFSNYAG
                     GIIGQTLAMEGVFAFFLESSFLGVLLFGESRCSRRILWVASAMLWVGSWLSGYFILAT
                     NAWMQHPVAYRVLDNGKLAIESLAELLTNPWLLWQFTHNMTAAVATGSFVMAAVGALY
                     LLAGRHLVHAKTYVQTGVVAGAVASALLITPTGHESARQVFEHQPIKGAAFEGLFRSE
                     RGADILLIGQPNLETMTIDNPLVVPSALSLLVYYDLYANVKGLDAFPPQDWPDHIALL
                     YFSYHIMAGLGTWLVVVMGLAFLWLWRGRLFASSWLLWLLLLSAPAPYIATTAGWMTA
                     ELGRQPWLVYGLFRTAEGISPLVHSGNALFTLLGFLGLYLALGLLFVLLFLEMLRRGP
                     IDHGATAGQSGEL"
     gene            33999..35048
                     /gene="cydB"
                     /locus_tag="PK589_06305"
     CDS             33999..35048
                     /gene="cydB"
                     /locus_tag="PK589_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNJ19246.1"
                     /translation="METLWFSALTFVLAMYIVLDGYDLGVAMLFPVVATDESERRMVR
                     ATIGPVWTGNEVWLIAGSGLLFLSFPKAYAAGFSGFYLGFIMVLWLLMGRGLAFELRG
                     HVEHPLWRRFWDTIFFVTSLSLAFLLGLFAGNVIRGVPLDADGYFFLPLWTDLRPGLN
                     PGILDWFTLLAGLTMVALLTLHGASYLAMKTVGRLQARSRALASWALLPAVLLVLVFV
                     VAIPFVRATFTVNFHTHPAGYLWVVLGLLAFVSLALSHTRQRQVAAFASSSVLLAVFI
                     AVIAWGTYPNLLVATTEPANSVTIHNASPGKDGLQAALWWLPFGLLVVVLYQIQIHRM
                     FAAPVTPDSRSVSDH"
     gene            35171..36274
                     /locus_tag="PK589_06310"
     CDS             35171..36274
                     /locus_tag="PK589_06310"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ19247.1"
                     /translation="MKAHRHHHPRVVVITGASAGVGRATVREFAREGAWIGLVARSRD
                     GLEGARKEVEAAGGRALVLPTDVAYPDQVEAAAAMVERRFGPIDVWVNNAMASVLSPA
                     LKMTAEEYQRVTDVTYLGYVYGTLAALRRMVPRDVGVIVQVGSALAYRSIPLQSAYCG
                     AKHAVQGFVDSLRSELLYDGSRVHITTVQLPAVNTPQFDWIKSRMPCHPQPVPPIYQP
                     EVIARAIVWASRHRRREISIGLPTVKAIVGQKFIPGLLDYYLAWKGYKAQQTNEPIAP
                     DRPNNLFHPLPGDHGAHGGFDEGAYEFSLQLWLNQRRLWVAAAALAVGGYLAKGLSTR
                     RNDRRWDGEPFSPTGGTTRSAREQAARQGGLYA"
     gene            36267..36539
                     /locus_tag="PK589_06315"
     CDS             36267..36539
                     /locus_tag="PK589_06315"
                     /inference="COORDINATES: protein motif:HMM:NF040444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HNJ19248.1"
                     /translation="MPEQKTMQRARQAKREGKAPTTQAGAFVEEEMEHMKRGKHRVQS
                     RKQAIAIGLSKARKAGVKVPAAGSKRATSASRRKSASRTRGSKRRE"
     gene            complement(36600..37664)
                     /locus_tag="PK589_06320"
     CDS             complement(36600..37664)
                     /locus_tag="PK589_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNJ19249.1"
                     /translation="MITASGAVILILIGLLLGLGEVIIRSVHLIRDGIPFFESNAGGR
                     IGPITLDQDLGWKATEHYQEALVEKTKNGTLYPVRRSQTQYGFRSFGDVSSRRPKMLV
                     MGDSFTHATAVSDDRTYHALLSKLLDVEVFAYGAGGYGTLQEYLVFDRYLDIVRPDVI
                     LWQYCINDFINNDNELERLSRVNNNGWVRPYWRNGTIQLLSPKESSLQIRDWINRHSR
                     FLYFVVTRLDRLRAANTRDTVEVDIEAEGMRHAGFARSVAVTDDLMGRVRARAGTRPI
                     MAFNCTEAEPYSQAFRDISGHHAIAYWGDVAQVVQDADTRGEDVFAADGSHWNERGHE
                     LAAQALAKHFKTALPPVPRP"
     gene            38143..38508
                     /locus_tag="PK589_06325"
     CDS             38143..38508
                     /locus_tag="PK589_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19250.1"
                     /translation="MMSQRLSNWSRRGWMVVMLAVGALTAGCMHHPGGIAPSTKPLAP
                     GGYTELGKVRGQDCVYHLLGLIPVTGGNEMRNAVEDALRTKPLADAMVEVTVDGYFQY
                     FILFSRACTQVYGTAVETK"
     gene            38637..39929
                     /locus_tag="PK589_06330"
     CDS             38637..39929
                     /locus_tag="PK589_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ19251.1"
                     /translation="MGNVAISVDRLTKRYSLGLQARHDTLRDRLAAALSWWKQCRSDD
                     TGKGREFCALTDVSFDVKPGEVFGIIGHNGAGKSTLLKILSRITAPTSGTADIYGRVG
                     SLLEVGTGFHPELTGRENIYLNGSILGMRRAEITAKLDEIIDFAGVEEFIDTPVKRYS
                     SGMYVRLAFSVAAHFEPDILIVDEVLAVGDAGFQQKCLGKMRQASRGGRTVLVVSHNL
                     PIIENLCQRALFLAQGRVALTGTARDVVQAYAGMTADLARSALDTRTDRQGKGHVIAT
                     AIEVLDQYRNVSSSAICGRETIVRWHYRCVPDKIIRRCRVELSLHARIGDPYETAYFL
                     MSTDLVSPLPLDLSGDGYIDFRVPEWPLSGGQYYVMSYVESDKEVQDWVHNASLLSVV
                     DGDFYGIGKSYPPNWRGKSVLVKYDWTQTAREAVHSFL"
     gene            40073..41434
                     /locus_tag="PK589_06335"
     CDS             40073..41434
                     /locus_tag="PK589_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ19252.1"
                     /translation="MSRSTTPLEIESHEQANIDLSSSARIRPPKVVHLSTYDVAGGAA
                     RAAYRLHDGLRRAGVDSSMFVREARTSDGSIIRFEPTTTFCKRAERRIRREQIERKLR
                     PAISPENDKIEPFRTDRSEYGTDILSKLPPYDILNLHWIAEFVDYPSFFGAFSKNTRV
                     VWTLHDMNAFTGGCHYDLGCGRYLGECGDCPQLAAAGPNDLSHEIWARKKSLFDQLSD
                     GQIHFATPSTWLAEEVQRSPILGRFPVSVIPYGLDLTEFSPRDVGSAREVFGIPPKAK
                     VILFVADGLPLFRKGFEVLVRALEHIRSWIPDLHLVTVGHNSPDLGHHIPHSYLGHIN
                     SDRLLSNIYSAADLLVIPSLQDNLPNTVLESMACGTPAVGFEVGGIPDMIQHCETGLL
                     VPPGEAGALGAAMLELLQNPERRMALGANCRQYALNHFSLEEQADRYCQLYTEILSER
                     AIR"
     gene            41477..41656
                     /locus_tag="PK589_06340"
     CDS             41477..41656
                     /locus_tag="PK589_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HNJ19253.1"
                     /translation="MFLKWAAVFFVLAMVAAAFGFTGIADKASDVAEILFYIFLAIVL
                     TFVVSGVVVARKLTA"
     gene            41986..42426
                     /locus_tag="PK589_06345"
     CDS             41986..42426
                     /locus_tag="PK589_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ19254.1"
                     /translation="MGMAWPFGLSTGDKVSLGRVLVVDDEDSIRSVLRMMLTQAGYDV
                     EEADDGGKAVEVLNSGDNPLMLDAIICDIRMPRINGVEAIAYFRAQYPSLPVIVLTGY
                     HDEPLASSLRRQGIVEFLEKPAEREQILASVAQAIAGRRISFYP"
     assembly_gap    42599..42698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <42699..43001
                     /locus_tag="PK589_06350"
     CDS             <42699..43001
                     /locus_tag="PK589_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19255.1"
                     /translation="SPATSAYEVHKLADGSGLLIGFMGKDVLMQVAPSQRPKSVRIAL
                     HSNPSEKAPHIVAVPLVKLAADMMPLRIDPKNPDSPVVFEMDLQSSSTRASTQGGQ"
     gene            complement(43109..43447)
                     /locus_tag="PK589_06355"
     CDS             complement(43109..43447)
                     /locus_tag="PK589_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19256.1"
                     /translation="MTRLTLSVCSLALGAILAMSSWCAAENVTTINILSPRNGDSVPE
                     TFEIKYEILNAPEGNHAHVYLDGAYQKGFKGTFTHVPKGEHTITVKIADHDHKDAGTA
                     TDSVTVKVGE"
     gene            complement(43471..44190)
                     /locus_tag="PK589_06360"
     CDS             complement(43471..44190)
                     /locus_tag="PK589_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNJ19257.1"
                     /translation="MQQVSGFIRSIQVGLPEQGHPHCHEDRPPQSWSTGIFKRPVRGP
                     IRLQQKNLEGDGQADLIHHGGVDKAVCVYPSEHWFHWSSVLPQQQLIGGEFGENFTLE
                     GLTEADVCIGDIFSVGSAVVQVSQPRQPCWKLARRWQIKDLAVQVEQTGFTGWYFRVL
                     QEGVVEPSIPLRLTDRPYPEWTVTTANRIMHQEHSNLKAAEHLSLCPLLSASWRHALH
                     QRSQQQNSPDPQPRQFGNSQS"
     gene            44317..45225
                     /gene="cynR"
                     /locus_tag="PK589_06365"
     CDS             44317..45225
                     /gene="cynR"
                     /locus_tag="PK589_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HNJ19258.1"
                     /translation="MEFRHLRYFLAVADALHFTKAAEGLPVSQPALSAQIKQLEQEVG
                     VPLFDRVGRSVQLTRAGSIFREHARRALREMELAQVAIAEEEGLQRGTLTVGVVQTVN
                     AYLVPEIVGRFSTLHPHVSLKFDELSGPDIEAGVKNGVLELGIGFVPPGSDRIATQPL
                     FEEDFVLIASPRHRFATRRHLPLASLADEPLVLLPGIFCTRRLLNASFEQAGIVPRVT
                     VEMNSIEGILATVKTSKLATVLPRLSLGLERHDGLRGIPLNNPTPRRALGLLWKKGGY
                     RSGAARALADQVRLVVNKRWKQKPGK"
     assembly_gap    45460..45559
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <45560..46177
                     /locus_tag="PK589_06370"
     CDS             <45560..46177
                     /locus_tag="PK589_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ19259.1"
                     /translation="VSFTGAQALLSDDAECIDQVLGGDIDRFGELIARHQHHVRKIVQ
                     GHVPADHVGEVAQDVFVRAYTGLAQYGGTVPFDHWLSRLSVRACYDFWRARRRTEIPV
                     SGLSEDQVGWLDRALAEDSTHQFRDQADQRDLLEVLEWALAQLSPEHRLVLTLVSLEG
                     RSVRDAAELLGWSVVNVKVRCYRARQALRTIFLREWERHRHDRPS"
     gene            46161..46481
                     /locus_tag="PK589_06375"
     CDS             46161..46481
                     /locus_tag="PK589_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19260.1"
                     /translation="MTVHRKPTLEQIEPLLVQAHRSRGEVPVGEDWAQEVMRAIRREA
                     AEHPAPSLLAWAEPVVWRVAAGAALVAVCFAGSVAIYTKQHAVPVAAVWLEEFDAGSA
                     FPDK"
     gene            46508..46936
                     /locus_tag="PK589_06380"
     CDS             46508..46936
                     /locus_tag="PK589_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19261.1"
                     /translation="MSRTGLWIGLSVLFCAGALMGAVGTSLYYQYEDQHRWERGPGAR
                     QERIMKRLTQELVLSTTQQAAVEPIVARAHVELLRLRVQHQPDVDRVLDQGMQELKAT
                     LSPEQQAKLDGLHAQLQQRWRMTREYVRQAQGAGGRKEER"
     gene            46936..48174
                     /locus_tag="PK589_06385"
     CDS             46936..48174
                     /locus_tag="PK589_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HNJ19262.1"
                     /translation="MAESVRFSTLLKRWMIGLSAACLLAVGTYVTMTRSGEAQPSRSE
                     KRPPTAARNTPVVAAVAKTGEINIYLNGLGSVIPMNTVTVKSRVDGQLMRVLFKEGQL
                     VRNGELLAQIDPRPFEVQLIQAQGQMARDQAQMKNAQLDFERYRDLYKQNFIPKQQLD
                     TQEALVRQYEGIVKADQGQIDNAKLQLTYSSITAPIDGRVGLRLVDAGNIVHANDPNG
                     LLVITQLQPITVVFALAEDHLPAVFERLKSGKQLVVEAFDREQKRKLATGTLLTVDNQ
                     IDPTTGTVRLKAVFPNDDSALFPNQFVNARLLLDIKQGATIVPSAAIQRGPKGTFVYV
                     VNEAEQTVSVRAVTVGVSQGEDTSIDTGLASGETVVVDGTEKLREGSKVEMRPSGGAV
                     PTSQESPPAGGERKKQGKRT"
     gene            48171..51284
                     /locus_tag="PK589_06390"
     CDS             48171..51284
                     /locus_tag="PK589_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNJ19263.1"
                     /translation="MNPSRLFIVRPVATVLTMIAILLAGALAYRHLPVSALPQVDYPT
                     IQVLTFYPGAGPDVMTSSITAPLERQFGQMPGLNQMTSTSSGGSSVVTLQFSLDIDLD
                     IAEQEVQAAMNSAATFLPRDLPAPPVYNKVNPADAPILTLALTSATLPLSQVRDFAET
                     RFAQKISQLSGVGLVSISGGQRPAVRIQANPRALAAYGLTLEDLRVVVAAANVNQAKG
                     GFDGPRRASIINATDQLFAGKDYKALIVAYRNGAPVRLSEVAEVIDDVENTKQAAWMN
                     ETPAVIVNIQRQPGTNVIEVVDRVKQLLPQLQGTLPSSVQVSVLTDRTTSIRATVRDV
                     QFELVLAVALVILVIFLFLRTLPATVIPAVAVPLSLIGTFGLMYLMGFSLNNLTLMAL
                     TISTGFVVDDAIVMIENIARYIEQGESPFQAALKGSKQIAFTILSLTVSLIAVLIPLL
                     FMGDVVGRLFREFAVTLSITILISAIVSLTLTPMMCARLLRERREEHPGPLARASQQM
                     LDRIIAGYGRSLQWVLRRQQATLLVTGATLVVTVALFVLIPKGFFPLQDTGVILGITE
                     GPQAVSFGTMAERQQALARVILEEPAVESLTSFIGVDGTNTTLNSGRVYINLKPLGER
                     VGGAGDIIRRLESRVADVEGITLHMQPVQDLTVEDRVSRTQFQYTLEDADPEELNRWA
                     PKLLEHLQLRPELRDVSSDQLNQGLASLVEIDRNTASRMGITPQLITDTLYDAFGQRQ
                     VSTMFTQLNQYRVILEVMPEFQKGPEALQALEVRGSGGSVPLNVFTRVTETTVPLVIS
                     RQGQFPAVTISFNLAPNGSLGAAVEAIRHVTQELGLPASIRGSFQGAAQAFQNSLEHE
                     PLLILAALITVYIVLGILYESYIHPLTILSTLPSAGVGALLALMLFRMEFSIIALIGI
                     VLLIGIVKKNAIMMIDFALEAERNEGKPPEQAIYEAGLLRFRPIMMTTMAALFGALPL
                     AMGAGVGSELRRPLGIAIVGGLVLSQLLTLYTTPVVYLALDRVATRFRRSREPVSVAG
                     ALPIGPP"
     gene            51281..54382
                     /locus_tag="PK589_06395"
     CDS             51281..54382
                     /locus_tag="PK589_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNJ19264.1"
                     /translation="MNISAPFVRRPVATTLLTIGATFIGIVAFQFLPVAALPQVEFPT
                     INVSASLPGASPEIMASSVAAPLERQFTRIAGVTEMTSSSTMGGTNVTLQFELDRDID
                     GAARDVQAAINASRVDLPPNLPSAPRYSIVNPADGPILILAITSELVTRGQMYDAASS
                     ILQQKLSQVEGVGQVAVGGGSLPAVRVDLNPTALNKYGIGLGDVREVLTSTNVNRPKG
                     QLTSDDRTWQIRTNDQLHKADEYLPLIVAYRNGRAVHLSDVASVESSVEDLRTMGVAN
                     GTPAVLVIVYRQPDANIIETVDRLRARLPQLEASLPGSMSLSIVMDRTPVIRASLHDV
                     ERTLAISVALVILVVFVFLRNLRATLIPAVAVPVSLISTFGVMYLLDYSLDNLSLMAL
                     TIATGFVVDDAIVVLENITRYREQGVPAMEAALRGAKDIAFTVVSMTVSLVAVFIPVF
                     LMGGMLGRLFREFAVTLSVAIGVSLLVSLTTIPMLCARVLKSETNVAHGWWYRISERG
                     FNVMLRGYATSLGWVLRHPRTMLIVTLATMALTIYLYVLVPKGFFPQQDTGRLFGNIR
                     AAQDISFQAMRDKLTEVVGIIKSDPAVASITGFTGGGSHSGTTNTGRMFIALKPLEER
                     GMSSEEVIARLRPKLAKVPGAPTYLQAIQDLRIGGRASSAQYQYTLQSVDLEELNTWA
                     PKVENKLRTLPEIVDVNSDQQDRGQQSLVIFDRSTASRLGLTPQLIDDTLYDAFGQRQ
                     VSIMYTPLNQYHVVMEVAPQYWQNPDALHEIYVRSPNGAQVPLSAVTRYEPANAILSV
                     NHQGQFPAVTVSFNMAVGVSLGEAVAAIDQAMHEIGLPASVRGTFQGTAKAFQSSVEN
                     QPWLILAALVTVYIVLGILYESYIHPLTILSTLPSAGVGALLALLLFKSELSMIALIG
                     IMLLIGIVKKNAIMMIDFALQSERLGEGKTPQAAIYEACLLRFRPIMMTTAAALLGAL
                     PLAVGMGVGSELRRPLGIAIVGGLLVSQVLTLYTTPVVYLLLDRLRLRWVRARESAPH
                     PAA"
     gene            54670..55713
                     /locus_tag="PK589_06400"
     CDS             54670..55713
                     /locus_tag="PK589_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNJ19265.1"
                     /translation="MSDIALRAHIVYERGSRSGGWLISLLLHGGLVALAMQAVMEVTP
                     HRQTETFRWDVALQSAPAPSPLAAEPLPPQQSVPQPPQPQKPVSRPVSQPSPEPVSQV
                     VERTAVVQTVQATQVTPRVVERSAPQERQAVATEVQPVTSQPVHETMVAHAQEILPQE
                     RQETVHTVEAQAVVTQVSEAMIQESAVPVATQSTAVEQPPIVEPAVVATEAPVETSAA
                     PVIEPQEAIVSEPVVVRQSAVVHREVREQAPAQADLGWLAESLWSRIEELKRYPRQAR
                     ARRWEGKVVLEAVIRHDGTILECLIAESSGHGLLDQDAISVLRKASPLTLKHPLGKEQ
                     ITILVPIAYRLES"
     gene            55753..56211
                     /locus_tag="PK589_06405"
     CDS             55753..56211
                     /locus_tag="PK589_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19266.1"
                     /translation="MKDVMQRVRRGLSSAVLCSVCLLWLGTGTALAESKGEKAFQAVA
                     TDAKGVETDVHGIIFYWEEKISETSFVPHELKEVPVKRGTATVKIKFDVIKAIDVKPS
                     GNGAPPAVSITLNDGKTGEFTLAIAGSFKGQSDFGEVELPAVELKRIAFK"
     gene            56268..56456
                     /locus_tag="PK589_06410"
     CDS             56268..56456
                     /locus_tag="PK589_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19267.1"
                     /translation="MTNSTKREPARMLTCDDVRCRCGQLVARWAEEGIEIKCKRCRRL
                     VTIAFGSIAGRPPAMLEN"
     gene            56598..>57384
                     /locus_tag="PK589_06415"
     CDS             56598..>57384
                     /locus_tag="PK589_06415"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNJ19268.1"
                     /translation="MARRIGVMGGGAVCVLVMGLVCGVSVHARAEGTEAAVPGQDASA
                     AKSNEEASGVVAESGETPKPKPVKIPEVMVRDVHERNEPDQESVKDIAGSVNVVTREE
                     IQRARPKNADEMLRRVPGVNVLDEYGQGLRPNIGIRGMDPRRSKNILLMMDDIPIQPA
                     LFGDASTYYMVPIERIDHIEVIKGGASVLYSPNTQGGLINFIQKRIPMTPTFSTTNTY
                     GSFNLFQSDTQYGGYWGNFGAQLGYLRRQSDGFRDRSKSQLDDF"
BASE COUNT        12803 a        15292 c        16327 g        12662 t
ORIGIN      
        1 gcacccgtcc ccagtttccc gactcccgtt ttgccttccg tcataagctg agccaccgga
       61 tagccgcgaa agccggtctc actctggaac cagtcgcccc caatcagcgc aatcacgtcg
      121 gcgacttccg ccttgggcag cgtgaaggcg agccaatcga tggtctgcgt gaaccctccg
      181 gagactgtca tttcttcccc cttggcttct gtgcgataga cgcccccgtc ttacccagtc
      241 gggggcgggt cggctgcgcg cgccgccggc tatcgccgcc ggtcgcgctg cgctgtccct
      301 ctttattctg aggcagaagc aaggacgacc tatgtccctt cgcctgcagg gcttccttca
      361 cgcgctccag gtcgaaccgc acaaaccggc aaaatcgatc tacgggaatc tcccctttgc
      421 ggtacgcccg tcggattgac ttcggactca ctttgaggac agcggccaac tcatcgacgg
      481 tcaaccatgt ttgctccatc gcatctcctt tacgtttcgg cgcacgatgc atggccgaac
      541 catacgcatc ggcgtgtggt ggtgaaagag gcccgttcgt ggacactagg ggacagcagg
      601 ggacactgag ggacaggagt tcaccgcaga aaagactaga atacagaagg ccgttaggag
      661 ggtatcgggg agagctcgaa agatcaccac gatctcctca tcgcagacgg cactcatgca
      721 ggccgctcca gacgtggcga aaacagcacg cctgaatttc agagcagcac accaagactc
      781 aggcgttatg tggaaggcgt gagtgtgtta aatacgtaga tgcagatatc ggcatcgttt
      841 tttgcgaaaa atcgtcacta gggccaaaaa atagttattg gggacaaatg gccctagcgg
      901 ctcgctttct tgcccgatct caactcgatc ttggataacc aggcgctgtc cggtcgaatg
      961 caagccagat gcgaaggaag ttagagaggc aacacgatca aaccgatatc aatacgtcgt
     1021 atggaaagaa ctgcagcggc aaagggagcc aaaaaaagta agttgaacag ccgataaatg
     1081 gtcctcctag agagacttac attcttaacc tagtcttttc tcccaagaat gcgtgctcgc
     1141 cgccaattat aagcaagtga tttcacagct taaaaaaggt atcttagctg tcaaaattcg
     1201 gcaggcgagg gtgaccaagc cctcgaagaa aaggacatat cctgttgctc cacatttctg
     1261 atcaagcatg gaactaatcg ataacattag ccgcctcctc ggcgacgact tgaaacaagt
     1321 tatccgccct ggatcgaggc tcaaggtagc tgcctcatgt ttctcaattt atgcgtttga
     1381 agcactcaag gcggagttag aaaaaataga atcactccag ttcattttca cctcacccac
     1441 cttcgtcccc ggagaggtca ccgacaagtt cagaaaagaa caccgcgagt ttcacatccc
     1501 caagctggag cgagaacgca gcttgtacgg cagtgagttt gaggtacgcc ttcgcaatga
     1561 attgacgcag aaagccattg ccaaagaatg cgccgagtgg atgcgccaga aggccagctt
     1621 ccggtcgaat cgcggcctgg cacccatgca acagttcgcg gctgtcacgt cgagcgggaa
     1681 agaatccata taccttccgc ttcacggatt tactgccgtc gatctcgggt atgaaaaggg
     1741 caatgccatc tccaacttcg tcaatcggat cgacgagcag cccctcacga caacctacct
     1801 ctcgctattc gatcagatct ggaacgatcc cgagaaatta gaggatgtca cggcgaggct
     1861 ctgcgaacac atcgcttcgg tgtatcaaga gaattcgccc cagcgcatct acttcctgat
     1921 gctctacaac atcttcagcg agtttcttga agacattagc gccgatgtgt tgcccaacga
     1981 ccgtacgggg tatcaggata gcctggtctg gaagaagctg ttcaacttcc aacgcgatgc
     2041 cgccacgggt gtgatcaata agctcgagac ctataacggc tgcattcttg ccgacagcgt
     2101 ggggctcggg aaaaccttca ccgcgctagc cgtcataaaa tactatgaac tgcgcaatcg
     2161 gtccgtccta gtcctctgcc cgaagaaact ggcagacaat tggctgacct acaaaggcaa
     2221 tcttgtcacc aacatcttcg ccaaggaccg ctttggctat gacgtgcttt gtcacaccga
     2281 tctgctccgt gagagcgggt attcatttgg gttagccctg aaccgtgtga actggggcaa
     2341 ttacgacctc gtggtcatcg acgaatcaca caacttccgc aataacaccg cctttgaaga
     2401 cagagagact cggtatcagc ggctgctgaa tcaagtgata cggcagggtg tgaagactaa
     2461 ggtgctgatg ctttcagcga ccccagtgaa taatcgcttt actgatttac gcaatcaact
     2521 cgcactggcc tacgaaggcg agccagagat tctcagtacc aagctcaagt cacaaaagca
     2581 tatcgacgaa atattccgcc gcgcccaggc ggtctttaat gcgtggtcca ctcttccgcc
     2641 agaagagcgt accgccgccg caatcctcaa tactctcgac ttcgacttct tcgaggtgct
     2701 cgacagcgtg acgattgcca ggtcccgcag gcacattcaa acctattacg acaccaaaga
     2761 cattggcacc tttcccgagc ggctgaagcc attgtccttt cactgccagc tcacgcaccg
     2821 aaaagacgtt atcggcttca acgaaatctt tgcacggctc aacaggctca cgctcgccgt
     2881 ctatgcccca ttgagctatg tgcagccaag ccggttatcg caatacgaga agctgtacga
     2941 ccccgatgcg gccatcggtc atggcaatct caagcagaca ggtcgtgaga aggggattca
     3001 ggcgttgatg accgtgaact tgctgaagcg gttggaaagc tcggtgcatt ccttccgcct
     3061 cacgttgaag agtcttgcag ggcatcaccg ccatgcactt gagaaaatcg ccgcgttcaa
     3121 gaaaaccggt cgcaacgcaa cctttgtcga tgtgtctccc gcctttgcgg aggcagagcc
     3181 ggacgaggaa agcgaattcc ccaatcctga tgatgcccag attggcaaga acgtacagat
     3241 caatctctcc gacatggatc ttccacgatg ggaggacgaa ctgcgtgccg atctcgtcat
     3301 catcgaggaa ttgctggcgg agatgcacaa ggtcacgccc tccgacgacg caaagcttca
     3361 gcatctcaag acgacaattt tgagcaagct agcctcgccc attaatgtag gaaacaagaa
     3421 ggtgctcctg ttcaccgcat ttgcggacac ggcggactat ctctatgagc acctggcgcc
     3481 cttcttttgg gaaaagaagc aactgcatac cggcaaggtc accggagcga acagccccaa
     3541 aagtacgctc aagaagacct acgacttcca atcgttgctc acgctgttct ccccactctc
     3601 caaggagaaa gctgccatac ttcccaagga gccgggtgag attgatctcc tcatcggcac
     3661 ggactgtatt tccgagggcc agaatctcca ggactgcgac tatctcatca attacgacat
     3721 ccactggaac ccggtccgta tcattcagcg ctttggccgt attgaccgca tcggctcgca
     3781 gaacaagtgc atccagttag tgaactactg gcccgacatc acgctcgacg aatacatcaa
     3841 tctcaaggag cgcgtcgaaa accgcatggt catcgccgat gtcaccgcca ccggcgacga
     3901 caatccgctc gactcccgtg ccaacgatgt ggcctatcgc cgtgagcagc ttcggcgcct
     3961 acaggaggaa gtcattgagc tggaagacct caagaccggc atctccatca ccgatcttgg
     4021 gctgaatgat ttccggatgg atctggtcag ttacgttaaa gcgcatggtg acttggcgag
     4081 cctcccgaac ggtatgcatg ccgtggtgcc ggctgatccc gagcgaggcc ttgtcccagg
     4141 cgtcatcttt gccctacgca atcgaaacga aggggtgaat atcaaccggc agaatcggtt
     4201 acacccctat tatctcgtct atgtcgcgca ggacggcaat gtcgtcgtcc accacacaga
     4261 cgccaagcgg ctcctcgatc tcgcgcgtgc ggcgtgtaaa gaccgaagcg aatccattcc
     4321 agaggtgtat cagtcgttta acagagccac tcaagacggc cgtcgcatgg atgcctactc
     4381 ggcgctcctg cagaagggca tccaatccat gatcgcgcag aaggaagaga aggacattga
     4441 cagtctcttt accggacgga agactaccgc tttactgaac accatcaacg gtcttggcga
     4501 cttcgagttg attgcattcc tggtcattca atcccgcgcg tgaccctgta tgtttgccta
     4561 tcccaagcag gccgagttca atcggctggt gccgaagtcc aagatctata cgtacgggcg
     4621 ggtctcaaag cgtatcaaag acctcttcgt ggcgcaggtc ggcgagatcc tctggaaata
     4681 caagctctcg ccggaaacga tcaatcttcc tgcacggaac ggcatcaatg aaattcaggt
     4741 attcgacatt gcgctcaaga aacccgaact cgacacagac attctccagg caatcgacaa
     4801 ggtcatcccg ttcccattag tgtttgaact cacgtacgat ggacaggtcc actttgcagc
     4861 atcatacaag cgtccgagcg aagccgatcc gtcaaagtgg gtcatcgagg ccacgtttca
     4921 aaccgatccg cagccggtga atgccgagcg caagccgctt cccgttgcgc ttgacctagc
     4981 cagtctttac gagcagattg tcgggtgcca tgtgccgttg ccgtcacggc agggggaagg
     5041 catcgcggag cacgtggccc gcttcaatga agtggaggcg aagaagaaga tgcggcaaca
     5101 attggagagc cggctcgccc aagaaaagca atttaaccgg aaggtggagc tgaacgcctc
     5161 actgcgctca ctctcacagg aactcgactc actcacccga cactgacgga tggctctatg
     5221 gacaagctca aaatgcacac gccgaatctt accgcggaga agatcgagaa ggtcgccgct
     5281 ttgttcccca actgcatgac cgagacgaaa gacgaaaaga cggggcaact caagcgggcg
     5341 attgatttcg accaacttcg ccaagaactc tctgaccaca ttgtggatgg accacgggag
     5401 cgatatcacc tcgactggcc aggcaagagg gaggcactgt tagccgcaaa cgcgccaata
     5461 gccaagacgc tgcgcccctg ccgtgaggag agtgtggatt tcgacaccac caagaacctg
     5521 ttcatcgaag gcgacaactt ggatgcgttg aagctgcttc aggagaccta ccttggaaag
     5581 gtgaagctga tctacatcga tccgccctac aacactggca atgattttat ctacgaagat
     5641 gacttcgcgg ccgacagtga tgagtatcag cttcgttcca accaaaagga tgccgttgga
     5701 aaccggatgg ttgcaaatac cgagtcaaat ggtcgcttcc attctgattg gctaaccatg
     5761 atgtattcga gattgaaact ggcgtttaat cttttgcgac ctgatggggc catttttatt
     5821 tcgattgacg atcatgaagt acataattta aggcgtgttt gtgatgaagt ctttggtgac
     5881 actaactttg tttccgacat agcttggcaa aagagataca ctcgcagcaa taacacagtc
     5941 gagttcacta ccgtaatcga gcatatttta gtatataggc gctccccaga attttgcgtc
     6001 aatcttcttc cgagaactga cgatgcggat gcccgctata ctaatccgga taacgatcct
     6061 cgagggccat ggaaaggcgc atcgtttctg aacccagcaa ctccacagga gcgtcctaat
     6121 ctctgctatc ccattcaaaa tccaaacaca caatcggtta cacatcctac gactaatgcg
     6181 tggcggcgtt cccgtgatga gtttgagcgg ttagcccaag aaaagaaact ttactgggga
     6241 ctcgacggca cttctcccat tccttccgtc aagatgtttc tttcggaagc acgtggtctg
     6301 acaccaataa acttttggag tcacgagtac gcagggaaca cagaccaagg gacacaagat
     6361 ctcgaaatgt tgatgggggg aaagtatttt aataatccga agccagttgt gcttatcaag
     6421 cgcattctgg agcatgcaac aggaccgaat gacttgatcc ttgatttctt tgcgggctct
     6481 ggaactaccg ctcaagccgt attgcagata aattcagaag atagtggggt gcgccgcttc
     6541 atcttggttc agttgcctga agctgctgat ccccagagtg cagcctgtag agctggatac
     6601 acgaccattt ctgacttagc caaggagcgt attaggaggg cagggtccaa agtatctaca
     6661 gataatggcc taattagtag caagcttgat gtcggattcc gtgtcctcaa gatcgataca
     6721 tccaatatga acgacgtata ttttgttcca gattctgtga ggcaagagga ccttttggcg
     6781 catacggaca atatcaaggg ggataggacg cctgaggatt tgctatttca ggtgctggtt
     6841 gattggggtg tggatttgtc catgcttatc gagaagaaaa caattggcgg gagtgccgta
     6901 ttcttcgtag atggcaacgc ccttgcagcg tgtttcgata tggatattgg agaggacttg
     6961 gtcaaagaac tcgcagagcg caagcctctg cgcgcagtct tcagagatag cagctatgga
     7021 agcgatagcc tgaaaatcaa tgtagaacaa atttttaaac tcttaacccc agctacagaa
     7081 gttcgatcga tctaaggcct cttttcttat aacaatcctt gaggtcggct gtatgccaat
     7141 ttcatccaga attagtggaa tcgatggccc tccgcttgga cagattttaa ggaccgaact
     7201 aattaatggt gaggatcggg ctttaggaat cgcatctgct ttcgttagcg taggcggact
     7261 ccgtgacata ttggcgatta caaagaagtg cacgtcgttg gagtgtcgac tgctcgcagg
     7321 tatcagcaat gcggtcacgc atccgaaggc cttaactgag gctctggatg cagggtggaa
     7381 agtgcgccta ggcacggcac ttggagctag gatatttcat ccgaagatga ttttgtccgg
     7441 gcgagtattt ctaagggatg gccgtgtaaa ggagccttcg tttgcgtatg tgggttcgag
     7501 caacttaaca aagggcggtc tttataagaa tatcgaatgc gggattattg ctcatgcaga
     7561 ctttgctccc ccgagtattt cattatgctt tgctaggctt tggaaagaag ggaaggcagc
     7621 gacccaagag agcattgagt cttacgccga agaatttgca agacgcaatc gaaagcgttc
     7681 attggacgac ctggtagagc ttggagtcgt cgacaatgct gaagatcaaa agtcaaccta
     7741 caagaatctt gctctccagc gtgacacgcc aaggcaaggg gtaatttcag aaggtgttgc
     7801 tggcgcagct tgggcaggcc ttgagtcgtt taccggtgac tatcaatttc aagttgaatt
     7861 tcctcaagcc gccggcattg tcttgaatag gatcattgga ggcagttcga gctccaaggt
     7921 acctatccta tgcaccaccg acaacacggt tcgtgaaatg acctatcgct attacactga
     7981 caatgggatg ttccgactga acataccaaa tgacacgcca ggtgttcacc gggcgcgaaa
     8041 aagtcataag ggaattgcgc ttattgaagt ctcagagagg cccgatgtta tcgcccaact
     8101 cacaatagtg cctcctggaa gaactctaga gcaaattatt agacgttcta ctttactagg
     8161 tacgtggggg agtacctcaa cacggaacta tgggtggtac tagtgcgaga tgtatagtga
     8221 ctcaaatagc gccgattcca atcgtggatc catttctgcc caaagtgtgc ttttaaaatg
     8281 aagctgaaat tcaaacagca gggctaccag acggatgccg tgaaggcagt ggtggattgc
     8341 ttcgccggtc agccgaagag ccgtggctcc aaatacgcga ttgaccccgg ccgtgccgct
     8401 gtaggcggca cagtaggact tccaatggag agcgagggct ttgcgaacgc cggcctcgcg
     8461 ctgtctccag tccaggtatt ggagaatctg caagaggtgc agcggcgtca gaaccttcct
     8521 atgtcatctg agctgaagaa gaccaccgtc tgtgatctga atcttgatgt cgagatggag
     8581 accggcacgg ggaagacgta ctgctacatc aagacgatgt tcgaactgaa ccgggagcat
     8641 gggtggagca agttcatcgt cgtggtgccc agcatcgcga ttcgtgaagg cgtgttgaaa
     8701 tcctttgaga ttatggcgga gcacttccta gagcaatacg gcaggcgcgc ccgctacttc
     8761 gtctataact cgaaacaact tcacaatctg gagagctttt cctccgacgc cggcatcaat
     8821 gtcatgatca tcaacgtgca ggcgttcaac gcccgtggcg aggatgccag gcgtatttat
     8881 gtggagctgg acgattttca atcccgtaaa cctattgatg tcatcaaaaa gaaccggccg
     8941 attctgatcc tcgatgagcc gcagaaaatg gaggggaaag ccacgacgga aaagttggcg
     9001 gagttcaacc cactcatgat tcttcgttac tccgccactc acaaaacaga acacaacaaa
     9061 gtgtatcggc tcgatgccat cgacgcctac aaccaaaagc tggtgaaaaa gattgcggtg
     9121 cgcggcgtca cggtcaaagg cttggcggga accaatgcct atctatacct agagactatc
     9181 cgtattgcga cgaccaagcc cccagaggcg cgtgccgagc tggagatctt gcaagccaac
     9241 ggtatcaagc gggtgcatcg attgctgaag aagggggacg atatcttcac gctttccgat
     9301 ggcctggagc aatatcgcgg gttcgtggtc tctgacatca acgctatcga gaatacgatc
     9361 agtttcacca atggcgtagt tctcggcgcg ggcgaggcca ccggtgatgt gaacgaatct
     9421 gctctgcgcc gcatccaaat tcgcgaagcc attaaggcac actttgaaaa ggagaaggtc
     9481 ttgtttccac aagggatcaa ggtcctctcg ctctttttta tcgatgaggt cgcaaagtat
     9541 cgaagctacg acggggcagg agagcacgca ggcgaatatg ccaagatgtt cgaagaggaa
     9601 tacgacgccc agctcaacga actgccgttg ctggaggaga gaccctacaa ccgctacctc
     9661 aagggtattc agacatccaa aacccacgac ggctacttct ccattgataa gaagaccaag
     9721 cggctggtga atcctgatgt gaagacgcgc ggcgaaacgg cgggcgaagc cgacgacgtg
     9781 gatgcctacg atcttatttt gagagacaaa cagcggttgc tgaccttcga ggagccggtc
     9841 cggtttattt tctctcactc cgccctgcgc gaggggtggg acaatccgaa tgtcttcgtg
     9901 atttgcacgc tgaagcatag cgacaatacg gtctcgcgcc gacaggaggt tgggcgtggg
     9961 atgcggctct ccgtcacaca aaacggtgat cggatggatg atccggcgat cgtccatcag
    10021 gtcaatgtgc ttaccgtagt ggcgaacgag agctacaagg attttgttgc cggcctgcag
    10081 aaagacatca gcgcatcgct atctgcacgg ccgaggcaag ccaacgagga atacttcagg
    10141 gacaaggtgc ttaaggctgc caccggcgat gtgaaggtca cgccgcacat ggcgaagttg
    10201 atcgagcggt atctcgtcaa gaatgagtat tccgatacca acgagagcat taccgagcgg
    10261 tatcacaagg cgaagaaaga aggagctctg gcgacgcttc ccgccgaact agaaccgtac
    10321 aaagagcaga tcttccagtt gatcgacagc gtgttcagcg cagcccagct acccgagatt
    10381 gaggatgacc ggaagggaaa ggacaatcac cggaacgcca acttcgataa gaaagagttc
    10441 caagaacttt ggagccgcat caatcgcaag gccatctatg cggtggattt caaaaccagc
    10501 gagcttattg agaagtgcat caaggcgctt gataaggaga ttagagtcac gccgcttcac
    10561 tacacagtaa cctctggtat acaaaaagac gaggcagatt acgacgaagt gaagaaaggg
    10621 gatgctttcg tcataagtga gaacctgacc gacaacgtaa ggagttcagc caggtcctcc
    10681 gtgaaatatg atcttatcgg caaactggcc gaagcgaccc agctcactcg acagaccatt
    10741 gccaccatcc tacgcggcat caatctcgcc gtgttcggtc aatacaaaac caaccctgaa
    10801 gacttcattt tgaaggccgg cactctcatt aatgaacaga aagccacggt gatcatcgag
    10861 cacttgacct ataatccgtt ggaagagacc cacaccatcg acatcttcgc gcagcaaaag
    10921 aaggaagaca ttagcaaggg attcaaagcc aatcgccata tatacgatta cgtctttacc
    10981 gattcgggta acgaacgcac attcgtcggc gaactcgatg ccagttcaga ggtcgtggtg
    11041 tatgccaagc tgcccaaagt cttctacatt ccgacaccgg tagggaacta caatccagac
    11101 tgggccattg cctttgaggc tggaaaggta aagcacgtct atttcgtcgc cgaaaccaag
    11161 ggctccatgt cctctatgga tctgcgtaag atcgaagaat ccaagatcga gtgcgcccgt
    11221 aaattctttg ccaagatcac ctcagatcaa gtgaagtacg atgtggtaga tagctacggt
    11281 aaattgatgg agatcgtgaa gtaggtgtga gccgaaggct ttcactcacc tcgctcaatt
    11341 ccagctctgc atatttcggc tgccatttat agaacgttgg ccgcaagttc gcttggagac
    11401 ttaacaccct aggtactttg tcaaatgggc ggatcaaatg gggccaacgt cagaggcatc
    11461 gaatttccaa caccgcgcag tctagttttt agggaagggg caaggccaac gtcagcagga
    11521 agaagagttg gattgcaggc taaatagcgt gaactaatct ttgaggccag aactcagtac
    11581 ttaatacttg tgcgaagcca ttaccgtcgt cgaattagtt ggacatgagt cgatttagcg
    11641 agttcggcca gttgagaatc tgcgcttttt agtaaccgaa gcgtttcctc aagggcgctt
    11701 tgggcaggtt ttgtagagga agtcttgacg atcgccacgg atggtacggt ttggctcatt
    11761 ggtcaccttc ttttttaggc aaagaaagca ttttgggtgc tggaggaggg attaaagcat
    11821 agggcaaact atcccacaaa gacgaacctg tgtcaatctt taggtcttgg tttgttctcg
    11881 ctttactttt caacttatta acaataaaag caacttccac aaattgatca atattttttc
    11941 gtgccagagc cagccactcc ttatggtagg ctcgtctcca gccatcgtct gaaggaggag
    12001 acgcattcac attgagcacc cctgcaacga gttttccacc taccgtgtcg gttttaggaa
    12061 caatgaaagg aagtgacacg aacatttgtt cctgaaattc gcttttcata tacgccgtcc
    12121 attcgacagc cagggcatct gggaccccct gaagcagggg gaggtcatct ttaaatactg
    12181 cggtcccctc ctgatgaata aatgctgttg gagctccggc aagtgaaagc cgagagccac
    12241 cagagtccaa aggaacccag aatcctaaat gcccagcttc agcagtctcg gcaataacga
    12301 cgaggcactt attcggttga gccaaatgtt tccaaagacg ggcagctcta gcatgatttt
    12361 gccttgctag ctcgttaggc aagaaaccat cgcattctaa gtcggcaact cgacatggaa
    12421 ccattaggtt ggcactcgct gggcaagcat ccgttttgtt tttgcccaga acatttagaa
    12481 ccgcctgcac ggcagcatac atagcccttt ctgaaatttg acctgccata tcaatagctt
    12541 gagctgactg tccatcgcta ataagaccat atgtttgatt ttgagcttgc cgaagggcaa
    12601 tcgcagtgag actcaactgt tcaggaatat cgcaggcctg ttcaatcgcg aaatccaaaa
    12661 gagcattacg cttttctatc cacaacctaa gttgaggatg ctgacgcatg aactctctgc
    12721 aatgatcgcg cagccctctc agaaaggtct tgctatctaa gtggtcgaag gcagtaagtt
    12781 tttgaaggct agacaattca tctaagcata gtgttggatt agctggatgc aaccatttcc
    12841 acatcgcgga ccgatatctc atctcatttg tgatttctct gccgagtctt acaaattttc
    12901 ctgcttggct tggtaggctc tccttcgact tatcaagtgc tatgtcggca ttccgaaacc
    12961 acctaagcca ggctggttgc tttactttat ctgcgtaata ggaaagtagc ttgtccgttg
    13021 agtaacttga aattagatca ttgtcggaca tctacacatc tccagatgtg attacggccg
    13081 tctaggttgc aagcaggtga ttacaaatac tctagtatat gtccgaacgc aatattccaa
    13141 caaagtacca acccctagtt attttaaatc ttctgatggt atcagtatcg taggtacggt
    13201 gggaaactgc catcgtactc taggtttgac ctaccgttgt tttccaatca gacgtcagcg
    13261 gcctagcgtc ggccatccct acacatccta tatcggacag tctatttgta agccattgta
    13321 ataccgtgta tgcgattgga atatttcagc acgtaagtca ttgattttac ttgtcgcctt
    13381 gggccctttt aaggcgaggg tcctgggttc gaatcccagc cgactcccca aaaatagcgg
    13441 aaataaaaga gaacatattg gcctaagttg ggcccggttg gttgaggata gtgtgtgatg
    13501 ggcacaattt aggcacaagt gcctgtccga ccgtgccgca ctgccccatg cgcgaaccct
    13561 ttttttcact caccactccc attcctttgc gctaaccttc cgacttcctc tgacccttct
    13621 ttttgttttg agcatagtgt cgaagctggg atttgacccg gtcgattttg aagatttaaa
    13681 attggcgcgg aatttaccag ccttttactt atctcaattt tctctcttcg tcggcattat
    13741 gagcacgtat ctaaatagat accatcacgt atcctaatgt gcacaaatta gcgaatcgct
    13801 cctccctagg agggctcata cgacggctca ataaacctgg tcaacacctt attagtaata
    13861 gcttttcaga ttaactggcg aatagtgagc gcactacact gagccaaggc gcaatgcttg
    13921 cagtcgtttt cttgagcgca agttgtgagc tgttacgtct gtccacgagg tgcccatgcc
    13981 aagcatccga ttcatcgcga atatgttact ggtttttctg gtgtcaacgg gcaatgcctg
    14041 cctagggcca gaagggcagg tttaaagcat cctacaagtg cagtctgctt cttgaaaaat
    14101 gctgtcccag ttccttcatt gaatagtgtc tgtgcagcta cttcagattg caagtccggc
    14161 ctaaagcgga atgatcagtg gtgccggaga aactccagct cgtaccgttg ctggaagtga
    14221 ggtgttgggt caagatgaag tgttgatgtg cccttctggg caaagttgtc gccccaaaat
    14281 agcgcagacg tacgagacat gctggatatt tttaaaatgt cgtggtccaa agttacaatc
    14341 atcggacttc ttttgtcagc aatgaagctc gcctttccgg ctggctcggt gaaggagtat
    14401 tcgtgaaagt agagtagcag ctgaacctcg cgaagatttc cttggctcat cattgcgaaa
    14461 ttaaggttga ggttataaat cagtcaatcg agcagagaga caccttcttc agaactgcaa
    14521 atgcggcgta agaaatgaat caaagtccga gtcatgatcg gaattagcat tgcgccgccg
    14581 ggcgccgcaa accttcattt ccgaggaact gcgcgtcgct attctgaaat tgacttcctt
    14641 tcgttcatcg ctctaaccaa ggaggtccat tttggcagac gcaaaagaat cagatctcac
    14701 tggctgcacg gaggaagtac ctcttctcgg tgtgacgcgc gaattcgatt tcgggcagtt
    14761 catcgagctt tatgacgact cttcagaccc aaacgatcct aagaatacac ccacgatctc
    14821 tcggatcgta atccagccca actcggagct ggccgctggc cgaaacgccg tgacggggga
    14881 gcctcctcct ataaaggtca gctcctctgc catatctgtc gcttctacag taaacacgcc
    14941 tcaaaacaat ggacaccagg tacccttcat cttagaaacc tccggaggcg caagttctcg
    15001 gtcggctaag gagccagagg ttcacctctt cgacttggct caatatggcc cggccatcaa
    15061 aaatgggccg atcgatgtcc agatcgtgga tgcggacaga tttctcgaga atggcacctt
    15121 cgagatgcgt ggacggactt acaaggtgaa ccttgaagaa gcctcaatgg atgccatcgt
    15181 gaagggcatg gctgtcacga caatcgcatc gtccggaaag gcctcgtcct cgacagtacc
    15241 cgtgcgcctg tttctgtcct ctgaaccagt caatatgacg ggtggtcaac tggccaaggt
    15301 cctatcgggc cagcctgtgg agtccagtat cggcgaagtg ctgccagctt tgacaacaag
    15361 cgcgatccgg acactattat cgggtgggac agtgctaacg cacggtgtag cctcagatgg
    15421 acggaagagg cccctcacgc tccatgtacc ggactctaat gcggaacccc tcacagacca
    15481 tttcgaagtc tttgacgtgg cgtcattcct tacaagcccc accgtcatgt ccaagggagg
    15541 gaaacacctg cccttaaagc tcatgcctga acaagtaaac cagctcattt cgagcggcca
    15601 tacccgtctt gagttagctg gtcgttccat tgacttcacc gtggtaaagg accaccagga
    15661 atcatcgata aggggtgttt ctagcttcaa agaggaccta aagaaaaaaa cgctccctca
    15721 gggagtcaga atccccggta tgagtttgga tgaaatggtc gcctatggca aacagttcga
    15781 gaaatatgaa ttcgttcatc cgtatcaacc taataagtac aacggtatcg cttctgaagc
    15841 gccctcggac gggtcggcca gcggtgcagg actacacagc atgacagaga aagttcagcc
    15901 gcgcctgccc tcgggttcgg gactccccgt ggccgtcttt gttccgtgga agcagacatg
    15961 gatgttgaaa ggattcagcc gtggaaatct gctacagacg atcgcgatgg cgcctcagga
    16021 acaaatcaca atgcaagtcc tgagctggga aagacgttcg cgctcacttg atcaatcttc
    16081 ggaaacagaa gttgaccagc aaactgatgt aacccaaacc acccgcgata ccgaggatgt
    16141 atttaaggag atgatctcta agcacgactt tgcatggcaa atttcaggat caatcgacgc
    16201 atcatacagc ccaggcgtcg catccatcca agtgcattct ggcggaggcg ttaccgacac
    16261 tagcaatatt gttcagacgg cacgccgaag cagccaaagc gtaaaggaat caaccgtcaa
    16321 agcctccagt cgcgtcaggt cacggcgtgt cacacggatt acccagacag ttgaaagcgg
    16381 cagggaggaa cgggtgacgc gtgtgattcg caaccccaat cagtgccata cactaacgct
    16441 agactttttt gaaactcttg ctcattacga gattaagcta gagttcttga aagaccgcct
    16501 gcgcttggtg gttctcgttc ccaatccaat taatgtacca gacttcttgt cagaaattat
    16561 ccgccgaaac gaaacggcgc ttcgcaacgc cctgatcgat acagctctcg ttgacggctt
    16621 cgaagcctcc aggctagttg cggcctatga agaagccaag atgctactgg cattgcaaat
    16681 ctccgaagcc gccaaggtga acgaggtgga gactcagcgt gacaagccca gcccatccgg
    16741 ttcaccggac ccagctgctc ctcagcagac ggagttagag cgggtggtcg gtgaaatgat
    16801 cagcatctta aagaatatga aggatgccga gattgacacc gcccttttgg ccattgctgc
    16861 ccatgctggt gtcccagaga ctgcccgcag aaagggccaa cagtggcttt tcatcaactt
    16921 ctgcaatgca aaattgcccg cgttgctctc cacgctcgat gacataacga aggggaatat
    16981 cgccccagta gtggcggccc agaaaatggt cgccgtcctt cccaaacccg atgcaccaac
    17041 gaatcttggg aatctcaatc agatgtccaa tgctgaaaag gagactgctt gtatcaccag
    17101 caagctgcgg gaggagacta atcaagagcg actttacatg aagagggatt gggactgggg
    17161 ttggtggtcg ggcaggatgc aggaggaagg gctttatacc gcaaacgacg gtggcctggc
    17221 agggctggca gatcagctcc agaaggccta tgccgcctgg gaagcgaaga aagcccaagg
    17281 cgacgcgatg aaggaccagg aggtggccaa gaccgaagcc gagggtaagc aggaaaaggc
    17341 aagtaccgac gacaagctcg ctatggcctt ccccctagaa gacctggcga gggcatatga
    17401 acggatgaag acattgatga gccacttaaa cgagcaccgg gcattctaca actacgcact
    17461 gttccaggcc ctgccaccca gcgagcagac agtacgaatt gtggaggcta gcaacggcag
    17521 gcttcaggtg ggcctcttcg aaccacgcgt cgtagccatg agtggcaaca agcttgtagt
    17581 gcctctaacc ccactggcga acaccacgca gctccaaaca ttcgtgacca gtctggcaaa
    17641 tgatctggaa aacgcgttca aaaccacact aaccacgccg gataccacag ttctgccgac
    17701 acccggagta tcggtctcgt ctagacttgg taagtgcagc gcttgtgagg actatatcga
    17761 aaaagcacgc gagcatgagc tcaagcgcct cgagggcctg gccagacagg agcaagccga
    17821 agctgaccga cgtaaggcgc gtctggaaaa gaatgacttc gacgactttc gtcaggcacc
    17881 ccctgcgatc aaattggatc tggacaagac ataatcgtca tggcagaccc aaacaatccc
    17941 tcacctcaca cggttcgact agcactggca attccgatat tgcaggcaga cttagaagat
    18001 gaagaggtgg agggatcttc accagcccca gctggtagtg aacctcccac ggatccctac
    18061 atggccttgg ccgcccggga gaaagcttgg gctatggttg caatctggcc ggaccaagac
    18121 tccgtccgga ttgagattcc atacgcagaa acagatgccc tctacgagct gatagaaacc
    18181 ctctatctaa ttggaggttg ggcagcagtt gataaagtct gcgaaagcac atcgattact
    18241 ctggtgggag cggtgtctca aatactgggt gtgcctaggg gcgaaaactt tgcgatcacg
    18301 tggctcctat cggatggcct ccttagaatt cccccggaac acctagatct ggcgaagaaa
    18361 gcctttcttg tcctcttagc ccgcgctgcg gatcggtcgt ggcctagcgt catgacaggc
    18421 ctacaggcta tcgttccctt tagtgggata ttctcgtacg gtccatctca cacaggtcca
    18481 tacccgtctg atgttcctaa ccctgaagta atcgcgcgat tatatgcgaa cgtggtggtc
    18541 aacctcgagt tcgtaagaag acaacttact acgcgcattc gtaatactct tcatcgcacc
    18601 gagcagaacg ctaaacgcct ccttagccag tcccttctaa actcacgagc agatattatt
    18661 cgggagacca tccgatattt cgactttgaa caaggaagta tcgaccatgt atttaagagc
    18721 acgaagcgta agtgggttaa gctttcttcg gaaccgggaa acatgaccag acctgctgaa
    18781 caaccagccg ctttacagct cgcccttaag aaactcgttc ccttcgcaca gagaattaac
    18841 aagcttaagg gtagtgctcg aggagcagga acaagcactc agaaaatggt ttctgaggct
    18901 ctccaaaacg ctcgatctat ctttgcccgc gagatgggcc gcctagcaca ggaattccca
    18961 atcttatctc aaatagagga gaaccatatc aaggatgcct cagaagccta cgctccgcag
    19021 ttgggttcca tccttttttc tatcctggaa cgggcaaatg acgctaacca tgagactaca
    19081 ctacgcttag acggctttgg cgatctcatc attacactga ctgaaggacg gcaccctgaa
    19141 caggatctcg ccgaggctgt ccagcgtctc ggtaaacgaa ataccatttg gggaatcacc
    19201 aaatatgtag agcgcgcaat ttcacaggcc actagcgtca cagacgaagt ggcacaaaag
    19261 gctttagcag atacagtgat gcttttggga attgagtctg gtgagacagt caaggctctt
    19321 gctcaaggtg cgatagaaat ggcagctctt ggtatggcca gcaatttcgc cactagattc
    19381 gttcctgtcc tcaatattgg attggctgct tggcacattg gaaaatctgt caaggaattc
    19441 tcggaacagg aagcggattt ctactgcgtc ttggaccctc gagactcgct ggtgtcggcc
    19501 gccccctcag tggccggcct caccatagac attgctacag aagctgtgtt cgcaatcatt
    19561 tagatttgca tatggctaac cccatcggaa caatactccc aaagaacaaa gtgacgggag
    19621 aatccatagc gcgattggtt tcggacgaag ataaggctat cgagaaaatt ctcgatggta
    19681 ttgtggagat gtcagatggt tcgcttgcta aagcccgtgt aactcgccct ctcactgagg
    19741 ccgcatgtcc agaaggctcg ttagtactta cacgagtctt gcgagagata gacgatctgc
    19801 tgggtcccgg aagatccgtc ctgcaggttt tgacggctcg atacgtgaag ttgcaatctt
    19861 ccttgcacgt tacgtcccgt ctactgcgac ttggccgtcg aacccggact cagtctgctc
    19921 gccagcttgt catcaccatg tttgaccgtc aactcaaaga gttctcagtt atcaaaggag
    19981 agatattgag agacatagag aagagtgtcg atgctgttgc caaacgtctt caggcacaaa
    20041 cggtaggcca gagagctacg aaggttagga aagatgtcca gacggcgctc gacgaagccg
    20101 aacaacaatt acagaaccaa cgcaaggtct tgacaggaaa gattgaggac tcatctagcg
    20161 ccattactca tcagaagagg gccgaagctc acgtcacaat ggtcatggct acaaaggata
    20221 caggagcacg cgcatttatc aattctcgca tcaaacgcct catgcaattt ccaaaagggc
    20281 gagaaatggc ggccgacttt gggaaggagc taaagaacaa ggtgcgccac tttttgccga
    20341 ttctggacga agtcgaggac tcggcccctt tctttatgga ggctttaggc atagtagcct
    20401 cagctaatgg aaagggaact agacttttgc gaagttgggc acaggggaaa actaaaattg
    20461 acagcgtctc cggttacctt gctcatatga aggggatttt gcctgaggaa gttgtaacta
    20521 gaatgaaatt ccttgagtcc atattccaca agaacgcctt tgagattctc tcagagttcc
    20581 ctccgaagct aagaggccag atgagcgtcg agatagtcga gggacctctg tgggttatcg
    20641 accactccgg agcgcccagg caatttgggg acagttgcat gttactcacc gggcccaatg
    20701 gtcaagcggc aataattggt ttagctgaag tcaaggcagg ttttgacgag gacctcttaa
    20761 agcaactgtt cgcaaggtct gatgcgcggg cactaaactc gagtgtaaca tttattgacc
    20821 gtaatgggaa aagtcaggtg cgcaagctca cgcgggagtt ctccttcgaa ggtggagaag
    20881 cagtccccat aaataaacca cccacctatg tctatggtcg aacaaaaggt gaaaagaagg
    20941 aaacagctga agagtttcag aaactggtga atgaccagat gcgcagagat agagaggtgt
    21001 ggaaagttcc tcttcccctt agcctccaac agaaccagcg cttctccgaa caggccctcc
    21061 gagaagcagt gaaggcaatc gtcaaagcag atccaaattg gggcctatga cgaacaacgt
    21121 ggtggcgatc agaataccta tgcagttagc actggaaaca gcggcttgct tatccattgg
    21181 tggccacaaa tgcactggtc caatgtagct tttgaccgga tactcgaggt ggctgctggt
    21241 ctgcaaaatc cttccaatat ggtgttaccg gcgcatccaa aaattgttac tggcatgcag
    21301 ccatattatc agtgagaatc tgcaaataga caattgtagc tcaacagtca tttctagcga
    21361 ccagacgccg agtaaattat gtcctgggaa ttccataacg cttagacagc atcctgttac
    21421 agcattctca gatagatggt tgggatgaga ttgcaaaaat ctctgcgtga gtccaggtct
    21481 tgtcctatat cggacagcct atttgtaagt cattgtaaaa ccgtgtatga gtttggaatc
    21541 tttcagcgcc taatgcattg atttacttat cccctggggc ccttttaagg cgagggtcct
    21601 gggttcgaat cccagccgac tcaccaccct tactcgaaac aagcctgtag tccctttcga
    21661 gaccgagacg atttagtttt ttcactcctc gtcaccataa acaaaagtcg ccgcttcctc
    21721 gccttcgccg gcccttacta gcgcgccaga gggtgccatg tttcgctgct ctacgtcggc
    21781 tctccgtatc accagggaac atcctagctc tcagtatgta gatgtgcatg gcataagaga
    21841 gcatgagatg gaaacgctta ctgttcctac tcatgatagc ggttgggtgt tcccgcccgg
    21901 tcgggggtgc gtggctcgct tgggcgcaaa ccggattagg tactcctcaa tcgagcactc
    21961 cagcggcgat ctctcctatc caaccttcat cagcgccgac gagcgggcta ccggctaaca
    22021 ttctgacacc ctcgaccacc tcccagctcg cgcccagctc ggggcctcgc gcgaacagtg
    22081 gccctccgcc tttcaccagc gcgggacgcg gcttgccggg tatgcaggga gggccaccac
    22141 tgaatgcgcc gatgggagca caagatcctt cccgaggtta tatgcagccc ccgactgtgg
    22201 ggccattgtt ctgtgatcct gccctgaata tccaatgttg aacggccgcg cctatgcttt
    22261 cctcaccgac atgcgaaaaa ccatgagtat ttttctgaac gcaatacaat ccgggatcta
    22321 cacaggagtc agcatggggt aggaacagct gaagaatcag tacggcgggc gggatcggac
    22381 gaaccagtca tggtcgacgt gtcctgcctg atatgcggag gtgtctatca tgaaacggtc
    22441 gcgtttgcgg cagagatcgc cgaacaaaaa gagctgctgc gaaggtttca ccaacagcgc
    22501 caacgtcagg acgcgcaggc ggacctcaag gaccgaacca cattctcgca agactattca
    22561 acggaggtgg tccgatgtct gacgtgtgga tttgtctatc gtaatcctcg gccgacgctt
    22621 cgatccgtca ccgctcagta tgcagacgac cagtatggcg aagagcatct cgaggcggag
    22681 tttcaggctc agcaaagatg ggcggagtgg aagattacag ccttcgcgca acactgtcac
    22741 ttcacttccc gtcatccggt agtcgtcgaa gttggaagtt ttgtcggggg ttttcttgcc
    22801 gctgcccaac aaagagggtg gtcaatcctg ggcgttgatc cggggaaaga ggtggtgaat
    22861 ttttgccagg cccgaggatt gccggtcttt catggcacgc tgaacgatgc caccatcgcc
    22921 gcccgatcgg ttgatgcggt ggtcatttgg aatacctttg atcagttggt gaatcccgac
    22981 acgaccctca gtgcagcgcg ccggctccta cgggaagatg gtcatctggt cctgcgggtt
    23041 cctaacggcg cgatgtttca gcaaggcgtg cattggttga aggccggtgg tagtcggcgt
    23101 cgttgggcta tcgtgaccct tgcgtggaat aatcttctgt cttttcccta cctcaacgga
    23161 tattcggttt cgactctgga tcaactggtc gcacgccatg gtttctctcg cgtggcggtc
    23221 gaagtggata cgctcatgac attgtcagac cagcacacga ctctgtgggg ccagtgggag
    23281 gaacgagttg tgaaatcttc ctgccgaatc ctggccggca ccaccaccct gcttccttcc
    23341 cttcacaata tggtcagccc ctggttggat gtctactacg ccgtcagtgg tgatgccgcc
    23401 acgtctccgc ccattgcctg tcctgcctga gcaaacgttt gtaaagagtg ttcgctaggg
    23461 gctttcccag atcatatggg acatgttcgc gcgtataagt gaggcgaata cctctttcac
    23521 gaacaatgac tattacccgc aatagcatcg aggatatttg gggcccgcgc acgccctatc
    23581 gcggacagtg gccggagcgg tctgactgta atgtggaagg aactccggag aagtgggtgc
    23641 cttccgcatg tgtgttgtgc tccaacgggt gtggagtgga aattggggtg agagagggaa
    23701 aaatcgtcgg tatccgggga cgctcagacg atcgggtcaa tcgaggacgg ttaggcccga
    23761 aggggctgta tggatggaag gccaatgcga gcccggaccg gctcacacac ccactgatac
    23821 agaaaaaggg caggtttcag gaagtcagtt gggacgaggc gatggaggtg atcgtccgaa
    23881 aatctcagga agttcgtcag cagtatacgg ccggggctat cggcttttac accagcgggc
    23941 aactgttcct tgaagaatat tacacgctag gcgtcatcgg aaaagccggt ttaggtactc
    24001 cacatatgga tggcaataca cgcctctgta cggccacctc atccgcggcc ctcaaagaaa
    24061 cgttcggctg cgacggtcaa ccgggttcgt acaacgacat tgatacgacc gacgcccttt
    24121 tcaatttcgg ccacaatgtc gcggagcaac aaacggtctt atggatgcgc atgttggacc
    24181 ggctttgcgg tgcgaatccg ccgaagcttg tcgtcgtgga tcctcggccc gtgccggctg
    24241 ccttgaaagc ggacgtccat ctcgcgccta aaccaggcac aaatgtggcc attatgaatg
    24301 gcctactgca tctcctcatc aaacatggat gggtcgatta ctcgtacctc agtgcccata
    24361 ccatggggtt tgagcaactg gaaaaggtgg tggccgcctg gacgcccgca cgggtcgcgc
    24421 aggtggcgga cataccggaa agaggcctgc gagaagccgc tgaaattctc ggcaccacgc
    24481 cgacgctcgt atcgaccgtt ctccagggcg tctaccaatc catgcaggcc acggcagcag
    24541 cctgtcaggt gaacaatctc catcttctca ggggactact ggggcgtgat ggctgcggaa
    24601 tctatcagat gaatgggcag cccacggcgc aaaatacgcg tgagtgtgga gcagacgggg
    24661 atttgccagg ttttcgcaat tgggataatc cgtcacatat cgccgacctc gccaggctct
    24721 ggaatgtcga gcctcatatc attccacatt gggcgccccc cacacatgcc atgcagatct
    24781 tccgctattg cgagcaaggt tccatcaaat ttctctggat ttcgggaacc aatcctgccg
    24841 tgtcactgcc tgatctccgg cgtaccaggc gtattctggg tcagaaggat ctgtttgtgg
    24901 tcgttcaaga cgcgtttatg accgaaacgg cctcgcgggc agatgtcatt ttgccggcag
    24961 cgatctgggg cgaaaaaacc ggatgcttta ccaacgtcga ccgcacggtc catttgtccc
    25021 ggaaggccat cgagccgcca ggggaagccc gtccggatct cgagatcttc ctggattatg
    25081 ccgagcggat gaactttcga gacaaggacg accaacctct catcaagtgg cacacagcgg
    25141 aagaagcatt tgaagcctgg aaggcatgct cgagagggcg cccctgcgac tatagtggta
    25201 tgacgtacga gcggctctca agccgctccg gcattcaatg gccctgtaac agcgagaatc
    25261 cggatgggac ctcgcatttg tacgtcgatg ggcatttccc ctccgacgcc gaatactgtg
    25321 agacctacgg gcatgacttt gactcgggtg ccgcggcgac gccggaacgg tatcgggccc
    25381 ataatccgca ggggcgggcg gtcatcaaag ccagcgacta tctagctccg tttgaagaac
    25441 ctgatgagcg atttccgttg tggctcacca cgggacgcgt ggtgtatcaa ttccacacgc
    25501 gcacaaaaac cgggcgcgtc gaagagctcc aagccaaggc cccagagccg tttgtggaga
    25561 tctcaagtca ggacgcggca gatcacggca tcgcggacgg cgagcgcgtg gaagtgcggt
    25621 cccgtcgcgg atatgtgcag atgcgggcga agataaccgg gatacgacca ggggttctct
    25681 ttatcccgtt tcactacggc tattgggacg agaacagtca tcctcgcgcc gcgaatgaat
    25741 tgacgctggt ggaatgggat ccggtgagca aacagccgca tttcaaatgt gccgccgttc
    25801 gtattggaaa gcccggcaag tccggccttg tgccgaagct ggtgaagaca tttgtcagaa
    25861 agacattcgg atgaatatcg gcgtctatgt tcgtttagct caccacagcg aaacgcagct
    25921 ggctgaggcc tttttgcagg tgtccgacca tcatcgggcc gagcctgatg tgcaggagac
    25981 ctgtcgcctc ctgtcatcct ggtccaaaga gaaatcgaac gggatccgtc cgtttatcgc
    26041 cacctacggg gaaccgaggg tgaacgagcc ggagacactc ttcagtgatc tgtttcaagg
    26101 ccctcgaacc ggagggctcg cgctcctgag agacttgcat gacctctggc tcctggccac
    26161 cgaagccatg atttcgtgga cggtgctgct gcaggcgtcg cgggcattac gggatgagga
    26221 tttggagcat ctctgcacta ggcatataac tcagaccgat cgccaactcg cttggctgca
    26281 gacacgtacc aaacaagctg ctcctcaggc gctggtggtc gcctagttcc ccctcagctt
    26341 cgtgcccgcc caaggggcgc tcccgagcag actcgtgatg cagcgacggc atgagcacgg
    26401 tttatggggc aaccgttcta cgaatcataa tttcctgtga gggtggtcgc agcaatggag
    26461 cttccgaatc aatccgctga gtcaatcggg ggcaggtccg gtctttcaga catggccctt
    26521 ctcatcatcg acatgatcaa cccgttcacc tttccccgtg cggaagatct ctttccggcc
    26581 gcctacgatg tggcggtacg catctgccgc ctcaagcaga aggcgaacca ggctgcaatg
    26641 cccgtgatct atatcaacga taatttcggg acatggcgcc atgattttca gaccctcgtc
    26701 cggcagtgtc gcgatgaacc atgcagagga cggggtattg ctgagctctt ggctccatcg
    26761 cagaacgact actttgtgtt gaagccaaag cattctgcgt tttttgccac tccgttggag
    26821 atcttgctgc aatcgctcag cgttcggcgt ctgatcctca ctggagtcac cggcgaccag
    26881 tgcattctct atacggcggc cgatgcgtat atgcgtgggt atggcttatt cgtgccttct
    26941 gactgtacgg catcgttgag tgctcaggtg aacgagcagg cgctggagca tatgcgtacc
    27001 tattttgacg cgaacacctg cctctccgag caactgatta cgacagctcc ggcggaggcg
    27061 ctcgccaagt gtgggactcc tcaatgagga agaggcgcgc accatcccgc tttgccagac
    27121 aatgcgggct agaaccagga tagccatgaa ccgcgacgcg aaggggtggg atgtcatcat
    27181 tgttggaagt gggccagcag gtctttctgc cgcgctatac ctcggtcgaa gccggcgacg
    27241 cacattgctg ctgcactctg gtcattccat ggccaaatgg gaagtgaatg tccaaaacta
    27301 tctcggattt ccgcaaggta ttgccggtac agatctgctc gagcgagggc tgacacaggc
    27361 gatgcaattt ggcgtagagg tgatcgaaga caccgtcgtc tcgctcagtg ttggcgatcc
    27421 gttccagctc gagggcggaa aaacccgtta tgaagccggg cggattcttc tggccaccgg
    27481 cctaacgcat gtgccgccgg atattcccgg cgtgaaggaa tgcctgggac acagcttgtt
    27541 tttttgtaag gattgcgacg cctaccgggt gcaaggtcaa cgaattgtca ttatcggatg
    27601 gaacaatgag gcggcagagt atgccttggg catgttgacg ttttcatcga acgtgaaagt
    27661 atgcacgaac ggccggcccg cggcgtggat acgacccatg ccgcgtggct caacgagtat
    27721 ggcgtgagca tatcgtcttc gcgcatccgt accgtccatc atgaaagcgg attcatcagg
    27781 tcgttcacgt tggagtccag tgaacagttc gaggccgacg ccgtatttac gactcgcggc
    27841 gatgtcgtac acaatcggct tgccgcccag gccggtgctg ccttgaatga agaaggtcag
    27901 gtggtggtgg atcattgcat gcaaaccagt gtcccgggct tgtatgccgc agggtgtctg
    27961 acaccggcga attgccagat gatcatcgcc gccggacaag gggccactgc ggggcaggcg
    28021 atcaatcgcg atctgtttga agagagtttg cgccgacatg cactcccaat gcgagcctcg
    28081 acgagggatt gccgggagag ccatcggttg atgtgctaga aggcgtgagg ttgtatggcg
    28141 atgttgaaaa gccattccgc tccgaatctc cctcaacctg aggccgcttc cgaggtcgcc
    28201 tcacatctcc tcgaagaatg ccgcatggtc ctccccggta ttcaagcgct attcgggttt
    28261 cagctcatcg cggtgttcaa taacaaattt ggcgagatgc cggaaggcca tcagacgttg
    28321 catttgctgg ctattgtgtt catcacagtt tcgatcgcct tcgtcatgac gccggcggcc
    28381 tatcatcggc aggcccatca gttcgcaatc agccgtacct tcgtccagat ttcttctctg
    28441 ctgctcttgt tgagtatgat cctgctcagc gtaggtatct gcctcgacgt ctatgtggtt
    28501 tgcgcgctca tcacgggaag tgacgcgatc agtctgatgg tggccctggc cgcagctttg
    28561 ctgttcgcat tgctctggtt tgtctttccg cgctgtatta ggaacccata aagtcctctc
    28621 tttgctccct gcatcgtcct cttctgtgac gaccacatga cgtggctccc ttcacgaact
    28681 agggatgaca cgagctctcc tgatggaatc tgtttgatag gctgttgcga gagtgtgagc
    28741 cgatttaacc cataaggagg ccgtcatggc agggaaaacc gcatttgcat cgggggttgt
    28801 tgagatgctg aaagaggacc acgccaaagt gaaagggttg ttcgaggaat ttgaatctgc
    28861 cgacggccag gagcaagcag aaatcgccgc gacggccatt tttgaattgg agatccatgc
    28921 cgaactcgaa gagaagctga tttaccccgc gatccgtgag gcgattgacg acagcgacat
    28981 gatgaatgaa gctgtggaag agcatcacct cgtgcacgtg ctcatcaaag aactgaagaa
    29041 gttaaagccg aaggatgaaa agtttcatgc gaagttcagt gtgttgagcg agctcgtcaa
    29101 gcaccacatt gaggaagaag aaggcgagat gctgccggag gctgaacgtc acgacatcga
    29161 ttgggatgcg cttgaagtgg cggtcatgaa acgcaaagag gtgttgatgg caaagtcctc
    29221 gaagaaatcc gggtcgagat caaaagccac tcccaagtca gggcgaagct gagtgtctgg
    29281 cggcggggta caaaccgggg ggatcgtctc taatccttct ctttgagagg agcatcgtat
    29341 gaggaaccac acaacattgg tgatcgcttg tctgacggga ggtgttctcg tgtgtgctgc
    29401 cctgccatcc ggcgcacaac attcaagcgg cggcacgccg ccggctgtca ctgaaggaaa
    29461 agcggggact catggcgggt ctggcagcca aggccaggga caagctgggc agactcaggc
    29521 tgggcagggc cagacaggtt cagggggaac tcaaggcagt aaaggaagca cagggggagg
    29581 gtcatcttcc tcagggtccg ggatggggtc ggggtccggc atgggaggtg ggacaggaat
    29641 gggctccgga agcaggggaa gctcgggtgg cagcggttct ggaggtggcg gcgggcggta
    29701 acgcccagag gtgggccacc gaagggcaaa actgagatgt attcgtatcg acggtgagca
    29761 ggcggcagcg agtggggcat gcattcctca tccgctgccg ccataggatt gagataggcc
    29821 ggcaaaggag gaccatgccc ttactcaaag aaataatgac accgagtgtc gaggtgatcg
    29881 ctcccaatgc gacggccgct gacgccgcgc gcaagatgcg agacctggat gtgggtgcta
    29941 tcccggtatg cgatggagaa aaactgttgg ggatggtgac ggatcgcgat ctcgttttgc
    30001 gtgttctggc cttgagtcga agcccggtcg atgccttagt gagtgacgcg atgacgccgg
    30061 ggttagtgta ctgcttcgag gatcaggatg ccgaggtcgc cgcggaactg atggcggaga
    30121 aacagatccg ccggataccg attctgtcaa aaaacaaaca actcgtcggg atcgtctcct
    30181 taggcgatct ggctatcgat ggattggatc agcacacgtc gggtgaagtg atccatgatg
    30241 tctctgatcc atcacgagga ggccattagt cggacgattc accggcagga atattctcgt
    30301 ttgccacaag gaggcgcaca atggccaggt acagcaaagg ctcgcagaaa accgtccagg
    30361 aagccatgca caagcgtaag aaggggacgc tcaaaagcgg cagcgggaaa aaggtgaaaa
    30421 gcaaaaagca ggcgattgcc atcgggttgt ccgaggctcg caaaaagggc aagaaagtcc
    30481 ccgccaaaaa atctgcgaaa tagactgacg cgtccgcagg actagggcgt cagcacgatc
    30541 ttcatgcagt ggtcttcctt gcggttgaat tttgcaaagg cctgcggcgc ttcgtcgagg
    30601 cgcatccgat gggtaataat tacactcgga tcgatctctc cgcgctgaat tctctccaac
    30661 aagggcgcca tgtagcgctg cacgtgggcc tgtcccgttt tgatcgtcaa tgagcgattc
    30721 atgatggacc cgatgggaaa cttgtcgata aacccgctgt agacgccgat gatcgagacg
    30781 gtgccgccgt ttttacagct catgatggct tgtcgtaagg catacggacg atcgctttcc
    30841 agcatcacgg cctgtttcat ccgatcatag gcggcaagga cgccggggcc gtgcgcttcc
    30901 atgcccacag cgtcgataca ggcgtcgggg cctcgaccgc cggtcatttc catcaaccga
    30961 tcatagacct cttcttcttc atagttcatg gtgtcggcgc ctccatcccg tgccatctgc
    31021 aggcgatcag gaaaccgatc gatggcaatg acacgttcgg cacccagaag aaacgcgcta
    31081 cggatggcaa attgtccaac gggcccgcaa ccccacacgg cgatcgtgtc accggattgg
    31141 atctggcaat tttcggcggc catgtatccg gtgggaaaaa tatcggagag gaacaacacc
    31201 tgttcgtcgg ggatgtccga gggaatttta atcgtcccca catccgcgaa gggcacccgg
    31261 gcatactccg cctgcccacc ggcgaatccg ccgagcatgt gcgagtagcc gaatattccg
    31321 gacggagaat gccccagcaa cttttccgcc atccacgcgt tggggttgga attctcgcag
    31381 agcgaaaata attcctgccg gcaggggaga catcgcccgc aggcaatcgg aaacgccgtg
    31441 acgacccgat ccccgactcg caagttggtg acctcgctgc cgacttcgac cacctcaccc
    31501 ataaattcgt gcccgagaat atctccggcc ttcatggtcg gcatcaatcc attgtataga
    31561 tgaaggtccg aaccacagat ggccgtcgaa gtcaccttca ggatggcgtc acggcgattg
    31621 aggatcgtcg gatcgggaac ctgttccacg cggacatcgc ccgcgccata ccaacaattc
    31681 gccttcatgg ggatcctccc ttgcttccgt cgttcacgca aatagacgaa cgataggttt
    31741 aagtgtgact cccggctggt tgcgccggcc gttgctggag aatccctttg ccatgtaacg
    31801 atccgtccga tctcagcact tcaccgactt caataatttg cttgagtcgg cgaagatcac
    31861 tctggacctg ctggccgggt tcgctgccga acagtttcgc aatggaagca cccagcaccc
    31921 cagccggcac gttgtattcc atcacgactc gcacaagagt cccccgtccg ccgggcgacg
    31981 gtgtgaattc caccgagccg gtgtggtgga tgtccgcctg cgaggtcgtc cgccacgcga
    32041 tctttcggtt cggttcgtcg tcggtgatct ccgcgttcca ttcaacccga tacccggccg
    32101 gtcctttcac cgtccaatga gaatggtgcc ggtcgtagat ggtcaccgat tccacgtgct
    32161 cggtgaagcg agacaggttt tggaaatcgc gccagaagag atagagcgct tcgggggaac
    32221 tgtcgatcat gatcgttttt ttgatcaaga cggctccgtc gtcactcgtc cagcccacca
    32281 tccggctcaa atgccggctc gcaaaacaat ccaccgccgc gattcccgcg acggccagca
    32341 tcgccgcggc cgttctcttg cggtttcctt gcgcactgga gagcgctgta gcgaggagcg
    32401 ccagatcgag ggcatctccg ccgacacggg accacatcca ccccgccggg cgtcgctccg
    32461 agaggatgcc aagccccatc gtaatctctc gtaatccaga ggctccnnnn nnnnnnnnnn
    32521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32581 nnnnnnnnnn nnnnnnnnnn nnnnnncgtg tttcttgtgt catcgcagaa gaggtcgtct
    32641 cggtttatgg acgtcgcgtt acagtatgac cgtctgcaat tcgccgtcac tgggacattt
    32701 cattatctgt ttccccaact caccatgggg ctggcgctgt tcgtcctgta ctttcgcacc
    32761 agggatctct tcagcgtcgg tgagcacttt catgaggtcg ctcgcttctg gaccggcatc
    32821 tttgccctga gtttcgcttt tggcgtcgtc accggtatcc cgttggaatt tcagttcggc
    32881 acgaattggg cggcgttttc caattacgca ggggggatta tcggtcagac gttggcgatg
    32941 gagggagtct tcgccttttt tctggagtcc tcgttcctgg gagtgctcct cttcggtgag
    33001 tctcgatgta gccgccggat tctgtgggtg gcgagcgcga tgttgtgggt cggttcctgg
    33061 ctttccggct atttcattct ggccacgaat gcctggatgc agcaccccgt ggcgtatcgt
    33121 gtcctcgaca acgggaagtt ggccattgag agcctcgcgg aactgctcac gaacccctgg
    33181 ttgctctggc aattcaccca caatatgacg gctgcggtcg caaccggttc attcgtcatg
    33241 gcggcagtcg gagcactgta tctcttggct ggtcggcatc tggtgcatgc gaagacctat
    33301 gtgcaaaccg gagtggtggc gggggcggtg gcctcggcgc ttttgattac tccgaccggt
    33361 catgaaagcg cgcgacaggt gtttgagcat cagcccatca aaggagcggc gtttgaaggc
    33421 ctgtttcgca gcgagcgcgg ggcagacatc cttctgatcg gtcagcccaa ccttgagacg
    33481 atgacgattg ataacccgct cgtggtcccg tcggcactca gtctgctcgt gtattacgat
    33541 ctctatgcca acgtgaaagg gctggacgcc tttcctccgc aggactggcc ggaccatatt
    33601 gccctcttat atttttccta tcacatcatg gccggactgg ggacgtggct ggtcgtcgtg
    33661 atggggttgg cattcctgtg gttatggcgc ggcagattgt ttgcctccag ctggctgctc
    33721 tggttgctgt tgttgagcgc ccccgctccc tacatcgcca ccactgccgg ttggatgacg
    33781 gcggaattgg gccgccagcc atggctggtc tatggcctgt ttcgcacggc ggagggcatc
    33841 tcgcctctcg tgcattcggg taacgcactg ttcaccctcc tcggttttct cggtctctat
    33901 ctcgccctcg gactgctgtt tgtattgtta tttctcgaga tgcttcgacg gggaccgatt
    33961 gaccatgggg cgactgccgg acagtcaggg gaactgtgat ggaaacgttg tggttcagcg
    34021 ccctcacctt cgtgctggcg atgtatatcg tccttgatgg atacgatttg ggcgtggcga
    34081 tgctttttcc cgtcgtggcc acagatgaat ccgagcgacg tatggtgcgc gccaccatcg
    34141 gccctgtatg gaccgggaat gaagtatggt tgatcgctgg aagcgggttg ttgtttctgt
    34201 cttttcccaa agcctatgca gccgggttca gcggattcta cttaggcttc atcatggtgc
    34261 tctggctctt gatggggcgc ggactggcat ttgaattgag aggccatgtc gaacatccac
    34321 tctggcggcg cttttgggat acgatctttt tcgtgacgag cctgtcgttg gcgtttcttc
    34381 tcggactgtt cgccggtaac gtcatccgcg gtgtgccact ggatgcagac gggtattttt
    34441 tcctgccgct ctggaccgac cttcgtcccg ggttgaaccc cggcattctc gattggttta
    34501 ccctcctggc cggcttgacg atggtggccc tgctgacgct gcacggcgcc tcgtatctag
    34561 ccatgaagac tgttggccgg ctgcaggcga ggagcagggc gctagcgagc tgggccctct
    34621 tgcccgctgt cctgttggta ttggtcttcg tcgtcgctat cccgtttgtg cgggctacct
    34681 tcacggtaaa tttccacact catcctgccg ggtacctgtg ggtcgtgctt ggccttctcg
    34741 cgttcgtctc cctcgccctc agtcacacgc gccaacggca ggtggctgct ttcgcttcgt
    34801 cgagcgtgtt gttggctgtg ttcatcgccg tgattgcatg gggcacctac ccgaatctcc
    34861 tggtcgcgac gaccgaaccg gcaaacagtg tgacgattca caacgcctct ccaggcaaag
    34921 atggcctgca ggccgccttg tggtggctgc cgttcggctt gctggttgtg gtgttgtatc
    34981 agattcagat acaccgcatg tttgccgccc ctgtcacgcc ggactcacga tctgtgtcgg
    35041 accattagct gaaatttcca tgcgctgagc gagcaaccat ccagggtttc gtccagttcc
    35101 aatccgatgc tctgggcgta cgctctcaga tcatcacgaa ggcgtctgtt cactcacgat
    35161 cgaaggactt atgaaagctc accgtcatca tcacccacgg gttgttgtga tcaccggagc
    35221 ttccgcggga gtcggccggg cgacagtgcg ggagtttgcc agggagggcg cctggattgg
    35281 attggtcgcc aggagccgcg atggactcga aggggcccgc aaggaagtcg aggcggctgg
    35341 agggcgggca ttggtgctgc cgacggatgt tgcgtatccc gaccaggttg aagcggctgc
    35401 ggcgatggtc gagcgccgat tcggaccgat cgatgtctgg gtgaacaacg ccatggcgtc
    35461 ggttctatcg ccggctctga agatgacggc cgaggagtat caacgcgtca cggatgtcac
    35521 ctatctcggc tatgtgtacg gcacattggc tgcgttgcga cgcatggtgc cgagagatgt
    35581 cggcgtgatc gtgcaggttg gctcggcctt agcctatcga tcgattccgc tccagtccgc
    35641 ctattgtggg gcgaagcacg cggtgcaggg attcgtcgat tccctcaggt ccgaactgct
    35701 ttacgatgga agccgcgtcc atattacgac tgtgcagttg ccggcggtga atacccctca
    35761 attcgactgg atcaagtctc gcatgccttg ccatccccaa ccggtccctc cgatttacca
    35821 gccggaagtg atcgccaggg ccatcgtctg ggcttcgcgc catcgtcgcc gggagatctc
    35881 gatcgggctg ccgacggtga aagccatcgt cgggcagaag tttattcccg ggctgttgga
    35941 ctattatttg gcctggaagg gctataaggc gcagcaaacg aacgagccta tagctccgga
    36001 tcgacccaac aacctcttcc acccacttcc aggtgaccac ggcgcacatg gcggcttcga
    36061 cgaaggcgct tacgagttca gtttgcaatt gtggctcaat cagcggcgtc tgtgggtggc
    36121 agctgcggca ttggcggtcg gagggtatct cgcgaagggc ctctccactc gacggaatga
    36181 tcggcgttgg gacggtgagc ctttttcgcc gaccggcgga acgaccagaa gcgcgcgcga
    36241 acaagcagca cgccaagggg gactgtatgc ctgaacaaaa gaccatgcaa cgggcgcggc
    36301 aggcaaaacg ggaagggaag gcgcctacga ctcaagcggg ggcattcgta gaagaagaaa
    36361 tggaacacat gaagcgcggc aaacaccgcg tgcaatctag aaagcaggcc attgcgatcg
    36421 gcctttcgaa ggcgagaaaa gccggtgtga aggtgcctgc agccggttcg aaacgggcca
    36481 cctcagcgtc tcgacggaag tcagcgtctc gcaccagggg ctccaagcga cgggaatagt
    36541 cgcaatggtg gggtacgttg gatgaaagac atcgtccgcc tgcgataggt gaggcgacgt
    36601 caggggcgcg gtacgggcgg gagtgctgtc ttgaaatgtt tcgccagcgc ctgggctgcc
    36661 agttcgtgcc cccgctcgtt ccagtggctt ccatcggcgg caaatacatc ctccccgcgt
    36721 gtgtccgcgt cttgaaccac ctgagccaca tcgccccaat aggcaatggc atggtgcccg
    36781 gaaatatcgc gaaaagcctg gctgtaaggt tcggcctccg tgcaattaaa tgccatgatc
    36841 ggccgcgtac ccgcccgtgc ccgaactcgt cccatcaagt catccgtgac agcgaccgat
    36901 cgagcgaacc cggcatgtcg catgccttcc gcttcgatgt ccacttcaac cgtatcccgc
    36961 gtatttgcgg ctcgcagacg atccagcctg gtgacgacga aatagagaaa tcggctgtgg
    37021 cgattgatcc agtctcgtat ctgaagcgat gattccttcg gagagaggag ttggatggtg
    37081 ccgttgcgcc agtagggtcg cacccagcca ttgttgttga cgcggctgag gcgctcgagc
    37141 tcattgtcgt tattgatgaa gtcgttgatg cagtattgcc acaggatgac atccgggcga
    37201 acgatatcga ggtaccggtc gaacacgaga tattcctgca acgtgccata gcctcctgcc
    37261 ccataggcaa agacctcgac atcgagcagt ttggataata aagcgtggta ggtccggtcg
    37321 tctgagaccg cggtggcatg ggtgaatgaa tcgcccatca ccaacatttt ggggcggcgg
    37381 gacgacacat cgccgaaact gcgaaagcca tattgcgttt gagagcggcg gaccgggtac
    37441 agggtgccgt tcttggtctt ttccaccaag gcttcctgat aatgctcagt ggccttccag
    37501 cccaggtcct gatccagggt aatgggaccg attcgcccgc ctgcgttgct ttcaaaaaaa
    37561 ggaatgccgt ctcgaatgag gtgaacggaa cgaatgatca cctcacccaa ccccaggagc
    37621 aggccgatga gtatgaggat caccgcgccc gacgcagtga tcatgacgag ttttctgccc
    37681 aaggtataca gccgcactct gcgagaggcg tgcgctccca cgccggaaca ttctctggtg
    37741 tgatcggctc acaccgccat catccggcag ccggcgaagg tctcatgttc cgtgaataaa
    37801 tactatcagg tattccctga caggcaatga aattgcccat gatgtgctcg gtggcgaggg
    37861 taaggttctg ctgttccgta atacctccat ttggagttcc ggcggcgacg tgccctgcca
    37921 agcagcccgc gagaggatgt cgcacattcg atcatgtgct aggccgtcgc aatttagggt
    37981 tgaggctgaa gggctatctg tgcaagatgg ggaaacgtgc tcgccgtact ccgccggttc
    38041 cttgcagtac caacttcctc atacatcctc acaacggtat gtccgacgtg gatgtcacac
    38101 tgcatgtcgt gaaggctatg cgcatgaaag gagtagaacc tcatgatgag tcaacgtttg
    38161 agcaactggt ctaggcgtgg gtggatggtc gtcatgttgg cggtgggagc cctcaccgcg
    38221 ggatgcatgc atcatccggg aggcatcgcg ccttcaacca agccgctcgc tccgggagga
    38281 tataccgaac tgggcaaggt gcgtgggcag gattgtgtct accatcttct cggattgatc
    38341 cccgtgaccg gcgggaatga gatgcgcaat gccgtcgagg atgcgttacg cacgaaaccc
    38401 ttggctgacg caatggttga ggtcacggtg gatggctatt ttcaatactt cattctgttt
    38461 tctcgtgcct gcactcaagt gtatggaacg gcggtggaaa caaaatagcc ccctcgttga
    38521 ggcgactcac gcttaatcca tccacccatc tgctagggaa agtccgaggt gatctgaaga
    38581 ccaatgtgtg acacataagt ggagcgaaat atcgtttatc cactacatgc ggacttatgg
    38641 gcaacgtcgc catctcagtc gatcgcctga caaaacgcta ttccctcggc ctgcaggcgc
    38701 gtcacgatac gcttcgtgat cgcctcgcgg ctgcgctctc ctggtggaag cagtgtcgtt
    38761 ccgatgatac cgggaaggga agagagtttt gcgctctcac tgatgtctcc tttgatgtga
    38821 agccggggga ggtgttcggc attatcggcc ataacggggc cgggaaaagt accctcctca
    38881 aaatcctgtc cagaatcacg gcgcccacgt cagggacagc cgatatctat ggtcgcgtcg
    38941 gttcgctcct tgaggtcggc actggctttc accctgagct gacggggcgc gagaacattt
    39001 atctgaatgg gtctattctc gggatgcgcc gggccgagat caccgccaag ttggatgaga
    39061 tcatcgattt tgcgggcgtc gaggagttta tcgacacgcc ggtgaaacgc tattcgagcg
    39121 ggatgtatgt gcgcttggcc ttttctgtcg cggcccattt tgagccagac attcttatcg
    39181 tggacgaagt cctggccgta ggcgatgcgg gttttcaaca gaagtgttta gggaagatgc
    39241 ggcaggccag tcggggcggt cggacggtgt tggtggtcag ccacaatttg ccgatcatcg
    39301 aaaatctctg ccagcgtgcg ctgttcctcg cacagggacg tgtggctctt accggtacag
    39361 ccagggatgt ggtgcaggcc tatgcaggca tgacggccga tttagccaga agtgcgttgg
    39421 acacacgcac ggacagacaa gggaaaggcc acgtcattgc gaccgccatc gaggtgctcg
    39481 accagtatcg gaacgtctcg tcgtcggcca tctgcgggcg agagaccatc gtgcgatggc
    39541 attaccggtg cgtgcctgac aaaatcattc ggcgatgccg cgtggaactt tcgttacatg
    39601 ctcgaatcgg cgacccctat gagacggcgt attttctcat gtccaccgat ctcgtgagcc
    39661 cgctaccact cgatttgtcc ggagacggct acattgattt ccgggttccg gaatggcccc
    39721 tctccggcgg ccagtactat gtgatgtcgt acgtggaaag tgacaaagag gttcaggatt
    39781 gggtgcataa cgccagccta ttgtccgttg tcgacggaga cttttacggg attggaaaaa
    39841 gttatcctcc caattggaga ggcaagtctg tgttggtgaa atatgattgg acgcagacgg
    39901 cccgcgaggc ggtgcactcc ttcctgtgat cactccattt cgagcggcct cgcgacgagt
    39961 gattcctcgg ggtatgccta gtggagcaag gagagttcag caggcatgat cgggactcat
    40021 ctcaagacat tttcccgagc accacggtgg aagatgaata gggaacccca tcatgtccag
    40081 aagcaccact ccccttgaaa tagagagtca cgaacaagcc aacatcgatc ttagctccag
    40141 cgctcgcatc cgtcctccta aagtcgtcca tctcagtacc tacgacgtcg ccggcggtgc
    40201 cgcgcgggcg gcctatcggc tgcatgatgg attgcgcagg gcaggcgtcg attcgtccat
    40261 gttcgtccgg gaagcccgaa cctctgacgg atctataatt cggttcgagc cgaccaccac
    40321 tttctgtaaa cgagccgagc gccgcatccg gcgtgaacaa atcgaacgca agctccgccc
    40381 cgccatctct cccgagaatg acaagattga accattccga acggatcgct cggaatatgg
    40441 gaccgacatt ttgtcgaaac tcccgcccta tgacattctg aacctgcact ggattgcgga
    40501 atttgtggat tacccctcct tctttggggc cttctcgaag aatacgcgcg tggtgtggac
    40561 acttcacgac atgaatgcgt ttacgggcgg ctgtcattac gatctggggt gcggccgcta
    40621 tctcggtgaa tgcggagact gtcctcagct ggctgccgca gggccgaatg atctctccca
    40681 tgaaatctgg gcccgcaaga aatcactctt cgaccaactt tccgacgggc agattcactt
    40741 cgcgactccg agcacgtggc tcgccgagga agtgcagcgc agcccgattc tcggccggtt
    40801 ccctgtgtcg gtgattccct atgggctcga cctcacggag ttctcgcccc gcgatgtcgg
    40861 cagtgccaga gaggtcttcg ggattcctcc taaagcgaaa gtcattctgt ttgtagccga
    40921 cgggctcccg ctgttccgga agggatttga ggttctggta cgggccttgg agcatattcg
    40981 cagctggatc ccggaccttc atctcgtcac ggtgggccat aatagccccg acctaggaca
    41041 tcatattcct cactcctacc tgggacacat caacagcgat cgattgctct ccaacattta
    41101 cagcgcagcc gatctgctcg tgatcccgtc gttgcaggat aacttgccga atacggtgct
    41161 cgagtcgatg gcctgtggaa cgccggcggt ggggtttgaa gtcggaggga ttcccgacat
    41221 gattcaacat tgtgaaaccg gcttgctggt gccgcccggt gaggcaggag cattgggtgc
    41281 cgccatgctg gaactgctgc aaaaccctga gcgccgcatg gcgctgggtg caaactgtcg
    41341 ccaatatgcc ctcaaccatt tttctctcga agaacaggcc gatcggtact gccaattgta
    41401 cacagagatc ttgagcgaac gtgccattcg gtaaagtcga gtgctgggat cgggaatctg
    41461 agccgaggag gtgggtatgt ttctcaaatg ggcagcagta tttttcgttc tggccatggt
    41521 ggctgcggca tttggattca caggaattgc ggacaaggcg tcggatgtag cggaaattct
    41581 gttttacatc tttctggcga tcgtcctcac ctttgtggtc tcgggtgtgg tggttgcacg
    41641 caaactgacg gcgtgagtcc ctcctctcta tagcggaccg tatcgctatg aggacatgtt
    41701 gatgtcgagg ccgccccagc tgtttacgcg gccggtgcat ctctgtgccg gccgctgtcc
    41761 ttcctgaacg tcttccaccc ctcaccatac ttcactgtga gctttggtgc atttgcaccg
    41821 aggtggtgtg attccaccac ttgaaagagg cgttccacgc gatggacaga agatctcgat
    41881 cgctaacttg ttgaatcggg tcatgtcgag tccgataggt cgaaggcctt gtccttgcat
    41941 cgccaaccca gggaaacaca tgcgtctggc gaagggaggg acaccatggg aatggcatgg
    42001 ccgttcggac taagtacggg cgacaaggtc agcctgggcc gggtcttggt ggtggatgac
    42061 gaagactcga tccgcagcgt gctgcgcatg atgctgacgc aggccgggta cgatgtggag
    42121 gaggcggacg acggcgggaa agcggtggaa gtgttgaaca gcggcgacaa cccgctgatg
    42181 ctggatgcga ttatttgcga tatccggatg ccgcgcatca atggtgtgga ggctattgcc
    42241 tattttcgag cccaatatcc atctctgccg gtcatcgtac tgaccggcta tcatgacgag
    42301 ccattggcct cctcgttacg ccgacagggc attgtggaat ttctcgaaaa accggcggag
    42361 cgggagcaga tcctggcatc cgtcgcccag gccatagccg gacgccgtat cagcttctat
    42421 ccgtgaggca agtgcactcc ttcctgtttg attttcgtga gtcggcttct tagaatcctc
    42481 cggaacgaca gctctccctt gttcaggagg atcagtactc atgtgtgaat gtatcaatcg
    42541 aaagatgttc caggcgccgc tccgtcatct cgcggccgcg ggcttggtgg gtgttcttnn
    42601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc tcctgcaacg agtgcgtatg
    42721 aggtgcataa gctggctgat ggaagcggct tgctgattgg gtttatgggt aaggacgtgc
    42781 ttatgcaagt ggcgcctagt cagcggccga agagcgttcg tatcgcactc cattcgaatc
    42841 catcggaaaa ggctccgcac attgtggcgg tgccgctggt gaaactcgcg gccgatatga
    42901 tgcccctccg gatcgacccg aaaaatccag acagtccggt cgtgtttgag atggatctgc
    42961 agagctcgtc cacccgtgcg tcgacacagg gcgggcaata ggcgctgagc actggtgagg
    43021 aaggcggcgg acagtgggcc gccttccggc cgtgcgattt tgaaagccgc accgtatcag
    43081 gcgcactctc gtcgcccgag gcttcctcct actccccgac cttcaccgtt acgctgtctg
    43141 tcgccgttcc tgcatctttg tgatcatgat cggcaatttt gaccgtaatg gtatgttcgc
    43201 ccttaggcac gtgcgtgaag gtacccttga accccttctg gtaggcgcca tccaggtaga
    43261 cgtgagcatg attcccctcc ggcgcattca aaatctcata cttgatttcg aacgtttccg
    43321 ggacggagtc gccgttgcgc ggagacaaaa tgttgatggt ggtcacattt tcagcggcgc
    43381 accaggaact catggcgaga atggcgccaa gggccagact gcagacggat agagtgagac
    43441 gagtcataac acctccggaa agaatggtga ttaggattga gaattgccga attgacgtgg
    43501 ctgcggatcc ggactgttct gctgctggct ccgctggtgc aatgcgtgtc gccaacttgc
    43561 agagagcaac gggcagaggc tcaagtgttc agcggctttc aggttgctgt gttcctgatg
    43621 catgatccga ttcgcggtgg tgaccgtcca ttcgggatac gggcgatccg ttaagcgtaa
    43681 cgggatgctt ggttcgacga caccttcttg gaggacgcga aaataccatc ccgtgaatcc
    43741 ggtctgttcc acttgcaccg ccagatcctt gatctgccag cgccgggcca gcttccagca
    43801 gggctgccga ggttgagata cctgcacgac agcggacccc actgaaaaaa tgtctccaat
    43861 gcacacgtct gcttccgtca gtccttcgag agtaaagttt tcgccgaact cgccgccgat
    43921 gagttgttgc tgcgggagga cgctgctcca gtgaaaccaa tgctcggacg gatacacaca
    43981 gaccgccttg tcgacgcctc cgtggtggat gagatctgct tggccgtcac cctccaagtt
    44041 cttttgctgg aggcgaatgg ggccacggac tggacgcttg aagatgccgg tggaccagga
    44101 ttgcgggggg cgatcttcgt ggcagtgcgg atgaccctgc tcaggcagac ccacctgaat
    44161 ggaacgtatg aaaccgctga cctgctgcat gtgatgctgt tcgatccgtt agacaacgac
    44221 gcgagtgacc ggcgtcaccc tagtgccagg cccgttcagg cgtcaaagtg tttgttctga
    44281 ttagatcaat cacttctagt tatgataaga ggtggtatgg aattccgtca tctccggtac
    44341 tttctcgcgg tggccgacgc gttgcatttt accaaagcgg ccgaagggct cccggtctct
    44401 cagccggcct tgtccgccca gatcaaacag ttggagcagg aagtcggcgt gccgctcttc
    44461 gaccgggttg ggcgatcggt ccaattgacc cgggccggat cgatttttcg ggaacatgcc
    44521 cggcgtgccc tgcgtgaaat ggaactggcg caagtggcca ttgctgaaga ggaagggctg
    44581 cagcgcggta ccctgaccgt cggcgtggtg caaaccgtca atgcctatct ggttcccgaa
    44641 atcgtcggac gtttctcgac gctgcatccg cacgtctcgc tgaaattcga tgaactatcc
    44701 gggccggaca tcgaagccgg tgtgaagaac ggagtcctgg aattaggcat cgggttcgtt
    44761 cctcccggat cggatcggat agcaactcag ccgctctttg aggaagattt tgtcttgatt
    44821 gcgtcacccc ggcaccgctt tgccacacgc cgccacctgc cattggcgtc tctcgcggat
    44881 gagcccctgg tgctcctgcc gggtattttt tgtacgcgcc gtttactgaa tgcaagtttt
    44941 gaacaggcgg gtatcgtgcc tagggtgact gtggaaatga attcgatcga agggattctc
    45001 gcgacggtga agaccagcaa actggcgacc gtgcttcccc ggctgtctct gggcttagag
    45061 cggcatgacg ggcttcgcgg gattccactc aacaatccca cacctcgccg cgctctcggg
    45121 ctgttatgga agaagggcgg gtaccgaagc ggggcggccc gggctctggc tgatcaggta
    45181 cgcctcgtcg ttaacaagcg ctggaaacag aagccgggta agtgactcgt agaccagggt
    45241 tgatagggca cggcccagtc agccttccgg cctgagcgat tgtgtgtggt cacgtgtaac
    45301 ctttgccggt ctatcaacga ctaacataat aagagggcca cgcctgcggt cctgagggcg
    45361 gatggggtat gatgctgagg tccatgctgt aagcagggag gacggtcagc tttacaggag
    45421 cgcaggcact cttgtccgat gatgccgaat gtatcgatcn nnnnnnnnnn nnnnnnnnnn
    45481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45541 nnnnnnnnnn nnnnnnnnng tcagctttac aggagcgcag gcactcttgt ccgatgatgc
    45601 cgaatgtatc gatcaagttt tgggcggtga tatcgatcgg ttcggcgaat tgatcgcccg
    45661 gcatcaacac catgtgcgca agattgtgca aggacatgtg ccggcggatc atgtggggga
    45721 ggttgcgcaa gacgtgttcg tccgggctta tacagggctt gcgcaatacg gaggcaccgt
    45781 tccattcgac cattggttgt ctcgcctgag cgttcgggcc tgttatgatt tttggcgtgc
    45841 ccgtcggcga acggagatac ctgtcagcgg gctgagtgaa gaccaagtcg gatggctgga
    45901 tcgcgcgctg gcggaagact cgactcacca gttccgtgac caggccgatc agcgtgatct
    45961 gctcgaagtg ttggaatggg cgttggcgca gctgtcaccc gagcaccgcc tggtgttgac
    46021 cttggtttcg ctggagggcc gaagcgtgcg cgacgcggcc gagctgttgg gatggtcggt
    46081 ggtgaacgtg aaggtgcggt gttatcgagc caggcaagcc ttacggacga tatttcttcg
    46141 agaatgggag cgtcaccggc atgaccgtcc atcgtaagcc tacacttgag cagattgagc
    46201 cgcttctggt acaggcgcat cgcagtcgcg gcgaagtgcc tgttggagag gattgggcgc
    46261 aggaggtcat gcgagcgatt cggcgggagg ccgcggaaca tcctgcgccg tcgctcctcg
    46321 cctgggcgga accggtagtc tggcgcgtgg ctgccggtgc cgcgctcgtg gccgtctgct
    46381 ttgccggctc tgtggccatc tataccaagc agcatgccgt tccggtcgcc gccgtctggc
    46441 ttgaagagtt tgatgcggga tcagcgtttc ctgataaata actcgttcgg aggctcgagc
    46501 gcgaggtatg tcacgcaccg gattgtggat cggactgagt gtcctctttt gcgccggagc
    46561 cttgatgggc gccgtgggga cgtcactgta ttatcagtac gaagatcagc atcgatggga
    46621 gcgggggccg ggagcccggc aggaacggat tatgaaacgg ctgacccagg aactggtatt
    46681 gtcgacgact cagcaagccg cggttgagcc aatcgtggcg cgagcccatg tggaattgtt
    46741 acggttgcgc gtgcagcatc aaccggacgt cgatcgagtg cttgatcagg ggatgcagga
    46801 attgaaggcg acattatcgc ctgagcagca ggccaagtta gacggactcc atgcccagtt
    46861 gcagcagcgc tggcgcatga cccgcgagta tgtccggcag gcgcagggtg ccgggggacg
    46921 gaaggaagag cggtgatggc tgagtcggtg agattttcca ctctcttgaa acgatggatg
    46981 atcgggctct ccgcagcctg cctattggcc gtgggaacct atgtcacgat gacccgctcc
    47041 ggtgaggcgc aacccagtcg gtcggaaaag cgtccaccga cggctgcgcg caataccccg
    47101 gtcgtggcgg ccgtggcaaa aaccggtgag atcaacattt atttgaacgg cctggggtcc
    47161 gtcatcccga tgaataccgt cacggtgaaa agccgggtgg atggccagtt gatgcgcgtg
    47221 ctcttcaagg aaggccaact cgtgcggaac ggagagttat tggcgcagat cgacccccgc
    47281 ccctttgaag tgcagttgat ccaggcgcag ggacaaatgg cgcgcgacca ggcgcagatg
    47341 aagaatgcac agctcgactt tgaacgctac cgggacctgt acaaacagaa cttcattccc
    47401 aaacagcagc tcgataccca ggaagccctt gtgcgtcagt atgaaggtat cgtcaaggcg
    47461 gaccaaggac agatcgacaa tgccaagttg caactgacct attcgagcat taccgcgccg
    47521 atcgacgggc gggtcgggct gcgcctcgta gatgcgggca atatcgtgca tgccaacgat
    47581 cccaacggat tgcttgtgat cacgcaattg cagccgatca ccgtcgtctt tgcgttggcg
    47641 gaagatcatc tgccggcggt gttcgaacgg ctcaagtcgg gaaagcagct ggtggtggag
    47701 gccttcgatc gggagcagaa gcgcaaattg gcaacgggga cgttgctcac cgtcgacaat
    47761 caaatcgacc cgaccaccgg gaccgtccgt ctgaaggcgg tgtttcccaa cgacgacagc
    47821 gcgctcttcc ccaatcagtt cgtgaacgcc cgcctgctcc ttgatatcaa gcaaggtgcg
    47881 accatcgtgc cgtcggccgc gattcaacgc ggtcccaagg gaacatttgt ctacgtggtg
    47941 aatgaggcgg aacagacggt cagcgtgcgc gcagtgaccg tgggtgtgtc acagggcgaa
    48001 gacacctcga tcgataccgg cctggcatca ggtgaaaccg ttgtggtgga tggaacggaa
    48061 aagttgcgtg aaggcagcaa ggtcgagatg cgtccatcgg gcggagcagt gccgaccagc
    48121 caggagagtc cgcccgcggg aggagaacgg aaaaaacagg ggaaacgcac gtgaaccctt
    48181 cgcggctgtt catcgtgcgg ccggtggcga cggtcttgac gatgatcgcg attctgctgg
    48241 ccggcgcgct ggcatatcgc catctgcccg tttccgcgct tccgcaggtc gattatccca
    48301 ccattcaagt gttgacgttt tatcccggcg ccgggccgga cgtgatgacg tcttccatta
    48361 cggctccact ggaacggcag ttcgggcaaa tgcccgggct caatcagatg acctccacga
    48421 gttccggggg cagttcggtc gttacccttc aattcagtct cgacatcgat ctagatatcg
    48481 ctgagcagga agtgcaggcg gccatgaatt ccgcggccac cttcctgcct cgcgatcttc
    48541 ctgcgccgcc ggtctacaac aaagttaacc ccgcagatgc gccgatcctc acgctggcgt
    48601 tgacctccgc taccttgcct ctgtcgcaag tgcgtgactt tgccgaaacc cggtttgcgc
    48661 aaaagatttc gcaattgtcc ggtgtcggtc tcgtaagcat cagcgggggc cagcgtccgg
    48721 cggtgcggat acaggcgaat ccgcgcgcgt tggccgccta cgggttgacg ttggaagatc
    48781 tccgcgtggt ggtggctgcc gccaatgtga accaggccaa gggcggtttc gacgggccgc
    48841 gtcgcgcctc catcatcaac gccacggacc aattgtttgc cggcaaagac tataaggcgc
    48901 tgatcgtggc ctatcgcaac ggcgcgccgg ttcggctgtc ggaagtcgcc gaggtcatcg
    48961 acgatgtcga aaataccaag caggccgcct ggatgaacga gacgccggcg gtgatcgtca
    49021 acattcagcg ccagccgggg acgaatgtca tcgaagtggt cgaccgcgtc aagcagttgc
    49081 tgccgcagct acagggaacc ctcccgtcat ccgtgcaggt ctccgtgctg accgatcgca
    49141 cgacttcgat tcgcgcgacc gttcgcgatg tacagttcga attggtgcta gcggtggccc
    49201 ttgtgattct ggtgattttt ttgtttctcc gcacattgcc ggccacggtg attccggcgg
    49261 tggccgtacc cctctctctt atcggcacct tcggcctcat gtacctcatg gggttcagcc
    49321 tgaataatct gaccttgatg gcgctgacga tctccacggg cttcgtggtc gacgatgcca
    49381 tcgtgatgat cgagaacatc gcgcgataca tcgaacaggg ggaatcgcct ttccaggctg
    49441 ctctcaaggg gtccaagcaa atcgccttca cgatcctgtc gttgaccgtg tccctgatcg
    49501 cagtattgat cccgttgttg ttcatgggcg atgtggtggg acggttattc cgggagtttg
    49561 ccgtgacgct gagcattacg atcctcattt cggcaatcgt gtcgctcacg ttgactccga
    49621 tgatgtgcgc caggcttttg cgcgaacggc gggaggagca tccggggccg ttggcgcgag
    49681 cctcgcagca aatgctggat cgcatcatcg cgggctatgg gagaagtctg cagtgggtac
    49741 ttcggcgcca acaggcgacg ctgcttgtga cgggcgccac gctggtggtc acggtcgcgc
    49801 tgtttgttct gattcccaaa ggattttttc cgctccagga tacaggcgtc atcctcggca
    49861 tcacggaagg cccgcaagcg gtctcgttcg gcacgatggc ggaacgccag caagctctgg
    49921 ctcgcgtcat tcttgaggaa ccggctgtgg aaagcctcac gtcatttatc ggtgtggacg
    49981 gcacgaatac gacgctcaac agcgggcgcg tgtacattaa tctgaaacct ttgggcgagc
    50041 gggtgggggg cgccggtgac atcatcagac ggctggaatc acgtgtggcc gacgtggaag
    50101 ggatcacctt gcacatgcag ccggtgcaag acctcacggt ggaagatcgg gtgagtcgga
    50161 cgcagtttca atacacgttg gaagatgccg atccggagga gttgaaccgg tgggccccga
    50221 aactgctgga acatttgcag ctgcgccctg aactgcgcga tgtcagcagc gatcaactga
    50281 atcagggatt ggcctcgctc gtggagatcg accgcaatac cgcgtcacgc atgggcatca
    50341 cgccgcaact catcacggat accttgtacg atgcgttcgg ccagcggcag gtttcgacga
    50401 tgtttacgca gttgaatcag tatcgcgtca ttctggaggt gatgccggaa tttcagaaag
    50461 gaccggaagc cctgcaagct ctggaggtga gaggcagcgg cggatcggtg ccgttgaatg
    50521 tcttcaccag ggtgaccgag accaccgtgc cgctggtcat cagccggcag gggcagtttc
    50581 ccgccgtgac gatctcgttc aatcttgcgc cgaatggttc gcttggcgcg gcggtggaag
    50641 cgattcggca tgtcacgcag gaattggggc ttcccgccag tatccgcgga agttttcagg
    50701 gcgcggcgca ggcctttcaa aactctctgg aacatgaacc gctgttgatt ctggccgcct
    50761 tgatcacggt ctacatcgtc cttggtattt tgtatgagag ctacatccat ccgctgacga
    50821 ttctctccac ccttccctcg gcgggtgtcg gcgcgctgct ggccctcatg ttgttccgta
    50881 tggaattcag catcatcgcg ctgatcggga ttgtgttgct catcggcatc gtgaagaaga
    50941 acgccatcat gatgatagac tttgccttgg aggctgagcg caacgaaggt aaaccaccag
    51001 agcaggccat ctacgaagcc ggactgttgc ggtttcgccc gatcatgatg accaccatgg
    51061 ctgcgctctt cggcgccttg cctctggcga tgggggcagg ggtgggctcg gaattacgac
    51121 gcccgctcgg gatcgcgatc gtgggaggat tggtcttgag ccagctcctc acgctctaca
    51181 ccacgcctgt ggtgtatctg gctttggatc gggttgcgac gcgtttccgc cgcagtcgcg
    51241 aaccggtgtc cgtggccggt gcgctgccga taggtccacc atgaacatct ccgccccctt
    51301 tgtccggcgc ccggttgcca ccacgttatt gaccattggt gcgacgttca tcgggatcgt
    51361 ggcgtttcaa ttccttccgg ttgccgcact accgcaagtc gagtttccga cgatcaatgt
    51421 atcagcctcg ttgccgggag ccagccctga gatcatggcc tcgtccgtgg cggctccgct
    51481 ggagcggcag ttcacccgga ttgccggagt gacagaaatg acatcctcca gcacgatggg
    51541 aggcaccaac gtcacactgc agtttgagct cgaccgtgac atcgatggcg ctgcgcgcga
    51601 cgtgcaagcg gcgatcaatg cctcccgtgt cgatctcccg ccaaacctgc cgagtgcacc
    51661 tcgctacagc attgtgaatc cggccgacgg gcccattctc attctggcca ttacctcgga
    51721 actcgtcacg cgcggacaaa tgtacgacgc ggcgtcgagc atcttgcagc agaaactctc
    51781 gcaggtggaa ggcgtcgggc aggtggcggt gggcggaggc tccttgccgg cagtccgtgt
    51841 cgatttgaat cctaccgccc tgaataagta tggcatcgga ctgggcgacg tccgggaggt
    51901 gctgaccagt acgaacgtca atcgtccgaa gggacagctc acctcggatg atcgcacctg
    51961 gcagatccgg acgaatgatc agttgcataa agcggatgaa tatctgccgt tgatcgtagc
    52021 ctatcgaaac ggacgggcgg tgcatctgtc ggatgtggcc tccgtggaat catcggtgga
    52081 agacctgcgg actatgggtg tcgcgaatgg tacaccggcg gtcttggtga tcgtctaccg
    52141 gcagcctgat gccaacatca tcgagacggt ggatcggctt cgcgcgcggt tgccgcagct
    52201 ggaagcgtcg ctgccgggga gcatgtcgct gtcgatcgtc atggatcgaa ctcccgtcat
    52261 ccgtgcctcc ttgcacgacg tggagcggac cctcgcgatc tcggtcgccc tggtcattct
    52321 cgtcgtgttc gtgtttctcc ggaaccttcg cgccacactc atccccgcgg tggctgtgcc
    52381 cgtgtcgctg atttccacgt tcggcgtgat gtatctcctc gattacagct tagacaacct
    52441 gtcgctcatg gccttgacga tcgcgaccgg ttttgtggtg gacgatgcca tcgtggtgct
    52501 ggaaaacatc acacgctatc gtgagcaggg cgtgccggcc atggaagcgg cgttgcgagg
    52561 agcgaaagac atcgcgttta cggtggtgtc gatgaccgta tccttggtgg cggtgttcat
    52621 tccggtgttt ctcatgggcg ggatgctggg gcggttgttt cgcgaatttg ccgtgacgct
    52681 ttcggtggcg atcggagtgt cattgctggt gtcgttgacg acgattccca tgctgtgtgc
    52741 gcgagtattg aagtcggaga ccaatgtcgc ccatggatgg tggtatcgca tcagcgagcg
    52801 gggattcaat gtgatgctgc gaggatatgc cacgagtctg ggctgggttc tccggcatcc
    52861 ccgcaccatg ttgatcgtga ccctggcgac catggccctt acgatctatc tgtatgtatt
    52921 ggtgccgaag gggtttttcc cgcagcagga cacggggcgc ctcttcggaa acatccgtgc
    52981 cgcgcaggat atttcgttcc aggccatgcg agacaagttg accgaggtgg tggggatcat
    53041 caagagcgac ccggcggtgg cgagcatcac cgggttcacc ggcggaggga gccattccgg
    53101 caccaccaat acgggccgta tgttcatcgc attgaaaccg ctggaagagc gcggcatgag
    53161 ttccgaggaa gtcattgccc ggttacgccc gaagctggca aaagtgccgg gtgccccgac
    53221 ctatctgcaa gctatccagg atctgcgaat cggcgggcgg gccagcagtg cgcagtatca
    53281 atatactctc cagagcgtgg atctggagga actcaacacc tgggcgccta aagtcgagaa
    53341 caagttacgc actttgccgg agattgtgga cgtgaatagc gatcagcagg atcgcggtca
    53401 acaatcgctg gtgatcttcg accggagtac cgcgtcacga ctcggtttga cgcctcaatt
    53461 gatcgatgac accctctacg atgcgttcgg tcagcggcag gtttcgatta tgtatacgcc
    53521 gctcaatcaa taccatgtcg tgatggaggt cgcgccgcag tattggcaga atcccgacgc
    53581 actccacgaa atttacgtcc gttcgccgaa tggtgctcaa gtgccattga gtgcggtgac
    53641 acggtacgaa ccggccaatg ccatcttgtc cgtcaatcac caggggcagt ttcctgcggt
    53701 gaccgtgtcc ttcaacatgg cggtcggcgt gtcgctcggt gaggcggtgg cggcgatcga
    53761 ccaggcgatg catgagatcg gattgccggc gagtgtgcga gggacgttcc aaggaacggc
    53821 caaagcgttt cagtcgtctg tagagaacca accctggttg atcctggcgg cgttagtcac
    53881 ggtgtatatc gtgctgggga ttctgtatga gagttatatt catcccctca ccattctttc
    53941 cacgctgccg tccgccgggg tcggggccct gttggcattg ctgctgttca agtctgagtt
    54001 gagcatgatt gccttgattg gaattatgct cttgatcggc attgtgaaga aaaatgccat
    54061 catgatgatc gatttcgcct tgcagagtga acgcctggga gaaggaaaaa ctccccaggc
    54121 ggcgatttat gaagcctgcc tgttacggtt ccgaccgatc atgatgacca ccgcggccgc
    54181 cctcttgggt gcgttgcctc tggctgttgg catgggggtg ggatcggaac tgcgtcgccc
    54241 gctcggtatt gccatcgtag gcgggttgct ggtgagtcag gttctcaccc tctacaccac
    54301 tcctgttgtc tacctgctcc tcgaccgtct gcgactgcga tgggtccgcg cccgagagag
    54361 cgctcctcat cccgccgcat agggactcgc acgatataca tggaagtgct tcgccgccgt
    54421 gagtagggcg atatgcgcat tgacaactgt ttcgcggtca tgatattgag aattgctatc
    54481 aagacttgtc gggagaaaag ccgactaaat cttggtcaag gacgcgggta gtgcgtcacg
    54541 ttcgcgcaac actcgaataa ggccagcgac ccctgcggtc agagcccaaa gctgtatcac
    54601 gacagctttg ggcttttttt atttactgtc ccgcggaccg gattgtctgg tcggggagga
    54661 ggtcggagca tgtcagacat cgcactcaga gcccatatcg tatacgagcg gggatctcgt
    54721 agcggtggat ggctgatctc attgctgttg catggcggac ttgtcgccct cgcgatgcag
    54781 gcggtgatgg aggtcacacc ccatcggcaa accgagacct ttcgatggga tgtcgccttg
    54841 cagtcggccc cagcgccgag tcctttagca gctgagcccc tccctccgca acagtccgtt
    54901 ccgcaaccgc cacagcccca aaagcccgtg tctcggccgg tgtcgcagcc ttcaccggag
    54961 ccagtatctc aggtcgtgga gcggacggca gtcgttcaaa cggttcaggc tacgcaagtc
    55021 accccgcgag tagtcgaacg ctccgctcct caggaacgtc aggcggtcgc gacagaggta
    55081 cagcctgtca cctcgcaacc ggtacatgaa accatggtgg cgcatgcgca agaaatcctt
    55141 ccccaagaac gtcaggagac tgttcatacc gtcgaagcac aggccgtggt gacgcaggta
    55201 tccgaggcga tgattcaaga gtcggcggtt cctgtcgcaa cgcagtcgac tgcagtggaa
    55261 cagccgccta tcgtcgagcc agcggtcgtt gcgactgaag cacctgtgga gacctcagcg
    55321 gctcccgtta tcgagccgca ggaggcgatc gtatcggagc cggtcgtcgt gcgtcaaagc
    55381 gcagtcgtgc atcgcgaagt gcgtgaacag gctccggcgc aggcagacct tggctggtta
    55441 gcggagtcgc tgtggtccag gattgaagaa ttaaagcgat atccccggca ggcgcgggca
    55501 cgccggtggg agggcaaggt cgtgctggag gcggtgatcc gccatgacgg aacaattctg
    55561 gaatgtttga ttgccgaaag ttccggtcac gggttgctgg atcaggatgc catctcggtg
    55621 ttgcggaagg cgtctcccct caccttgaaa catccgctgg ggaaagagca aatcaccatt
    55681 ctcgtgccga ttgcctatcg gcttgaaagt tagtaacagc aacatcatca caaggggcaa
    55741 aggagtttca ccatgaagga tgtcatgcag agagtgcgga gggggttgtc gagcgctgtc
    55801 ctgtgcagcg tatgcctgtt atggctgggg actgggacag ctcttgctga gtcgaaaggc
    55861 gagaaagcgt ttcaggcggt ggcgactgat gccaaagggg tggagaccga tgtgcacggc
    55921 attatttttt attgggaaga gaagatcagc gaaacctcgt ttgtgccgca cgaactgaag
    55981 gaagtgccgg tgaagcgcgg aacggccaca gtcaaaatca agttcgatgt gatcaaggcc
    56041 atcgatgtga aaccgtccgg aaacggggcg ccgccggctg tctcgattac gttgaacgac
    56101 ggcaagaccg gcgagttcac cctggcgatc gccggcagct ttaaggggca atcggatttt
    56161 ggcgaagtcg aattgccggc ggtcgaactc aaacgaatcg cctttaaata aatgcggcgg
    56221 cgaattgagc gtgacggaac agaaggccgt cgagtggagg aattggcatg acgaacagca
    56281 cgaagcgtga acctgcccgg atgttgacgt gcgacgatgt gcggtgccgg tgcggacagc
    56341 tggtggctcg ttgggcggaa gagggcattg aaatcaaatg caagcggtgc cggcgtctgg
    56401 tcaccattgc ttttgggtcg atcgcgggga ggccgccggc gatgctcgag aattaatcca
    56461 tcctcagccg atcatgccat agaggccaaa ggaccgtgtg tgtccagagc ccaagaccat
    56521 caacagggtc ttgggctttt ttatgtgtgc tgatgtcttt ctacgtgtcg gtgaatgaac
    56581 aaggagaaca agtaatcatg gcgagacgta tcggggtcat gggcggaggg gcggtatgtg
    56641 tgttggttat gggacttgtc tgcggcgtga gtgtccatgc gcgggccgaa ggaacagagg
    56701 cggctgtgcc tggtcaggat gcctctgctg ccaagtccaa tgaagaggcc tcaggcgttg
    56761 tggctgaatc gggggaaacg ccgaaaccta agccggtgaa gattcccgaa gtgatggtga
    56821 gagatgtcca tgaacgaaac gagccggatc aagagtcggt gaaggacatc gcggggtcgg
    56881 tcaatgtcgt cacgcgcgag gaaattcagc gggcccggcc gaaaaacgca gacgagatgt
    56941 tgcgacgcgt gcccggtgtg aacgtgttgg acgaatatgg ccaggggttg cggccgaaca
    57001 tcggtatccg ggggatggat cctcggcgca gcaaaaatat tctgctgatg atggacgata
    57061 ttcccatcca gccggctctc tttggtgatg cctcgacgta ttacatggtg ccgatcgaac
    57121 gtatcgacca tatcgaggtg atcaaaggcg gcgcgtccgt gctctattca ccgaacacgc
    57181 agggaggcct gattaatttc atccagaagc ggattcccat gacaccgacg ttttctacca
    57241 ccaatacgta cggcagtttc aacctatttc aatcggacac tcagtacggg ggctattggg
    57301 ggaattttgg cgcgcagctc gggtatctcc ggcgccagtc agacggattt cgagatcgaa
    57361 gcaaatcgca gttagatgat ttca
//
LOCUS       DAOQDS010000019        57170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_171_length_57170_cov_20.080539, whole genome shotgun sequence.
ACCESSION   DAOQDS010000019 DAOQDS010000000
VERSION     DAOQDS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 57170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57170
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171_length_57170_cov_20.080539"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PK589_06420"
     CDS             <1..873
                     /locus_tag="PK589_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNJ19269.1"
                     /translation="QLSAISRPRIKAWAAGLLEKGLDFDTALNALLTLSAVLTEAVED
                     GLITHNPALRSGKLLKRPATIEESELAIFTPEEEGLLLETIRRDRPLFYPMALTFFRT
                     GLRAGEVLGLHRGDLNFHSRSIYVQRNWSRGILGTPKNGKARRVDMSQGLAKALTEWI
                     ELQDLEAAAAGHAAPEVLFPGNLGGTRREPSYMAENWLRYKLWFPMVEKAGLRRLDMH
                     SVRHTYASRLIANGENLKYVQEQLGHASITITCDTYGHLIPGGNRQAVDKLDSLKIGS
                     GTVTKTVTNAYENS"
     gene            complement(870..1064)
                     /locus_tag="PK589_06425"
     CDS             complement(870..1064)
                     /locus_tag="PK589_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19270.1"
                     /translation="MQYWVKVVFADNQELLVKDAIRHTISEDMEVLEVDSAKEVIIVP
                     MKQIKYIACDATVFAQKSKT"
     gene            complement(1080..1910)
                     /gene="proC"
                     /locus_tag="PK589_06430"
     CDS             complement(1080..1910)
                     /gene="proC"
                     /locus_tag="PK589_06430"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNJ19271.1"
                     /translation="MLTGRQIGFVGGGNMAESLLAGLLRKGLSSPGRLVVSDPLASRR
                     ELLSRNLGVSVTPDNQAAVRGADIVVLCVEPQVLDDVLRELAPSLPSDPVIISVAAGY
                     PLSRVQHHLPSATRLIRAMPNTPSAIGEGVTAMSLAPGVSSEDRETARQLFESVGMAV
                     VVEERLLDAVTALSGSGPAYVFAMIEALADGGVLGGLPRSTAQLLAAHTVAGAASMVL
                     EQGEHPAVLKDRVASPGGTTIAGLYQLEQRKLRATLMSAVEAATQRSQELGKTENHHL
                     "
     gene            2027..3103
                     /locus_tag="PK589_06435"
     CDS             2027..3103
                     /locus_tag="PK589_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ19272.1"
                     /translation="MRCILSLVILGVLLLFGDPSWARREALTAEQKSQMERIDRVLIT
                     VLALSDKGSVDAGPLAEVAANRMKEFGYTPVTDPTQPHDVELKIKCEQKKTWEGTTTM
                     GSDADLPDAPSRIWKGPACQLGYLLNGKKMAWRKEVRTDFDDAQAAATLAKAGDPTSF
                     AMGRLQARLEEYDFPALITAEWGQEARLFRLYDDPKTPVARKVKLVALFGDLFSVKAI
                     PRLLDGLNGPDRSIAKASALALGNIGQKDTIPVLIKTMKNGAPDLRAPAAKALGIVGA
                     LHGDFTIVDPLLETLNTEDVAVKTEVAWALGKLPDMRAYEPLFALQKSLYHVHENDAD
                     PNLVKLKEAVNWSIKQIDTWEHVQ"
     gene            3100..3642
                     /locus_tag="PK589_06440"
     CDS             3100..3642
                     /locus_tag="PK589_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19273.1"
                     /translation="MRNGIHIGRCEQCLAASILSFFILVAAAGIGQAQVIGEEAELDR
                     LRAKAEDAMGNDDAEGAAMNMGRAALMAAQLGKRQTEPALRQVFKSTEHLYRSQEHGY
                     RGLALFRRAGGELPASAGVCGSLQLAQLELQHAQETLASSETPDRPGAVSPKLPAIRQ
                     TADDWAIVLASMMGEFRCPN"
     assembly_gap    3692..3791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3986..4429)
                     /locus_tag="PK589_06445"
     CDS             complement(3986..4429)
                     /locus_tag="PK589_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNJ19274.1"
                     /translation="MPYYYFDSTALVKRYSMERGTRIVNKLMVKRGKVAILPMWSVTD
                     FYSALSVRSQQGEITRDDCYSVLYKFEMEASQGLFQFISPTMDTYLAAKELILDYPAL
                     RSPQLLHLALALELKPLRLTVVSADKQLLAACRPAGLHIINPEDD"
     gene            complement(4431..4679)
                     /locus_tag="PK589_06450"
     CDS             complement(4431..4679)
                     /locus_tag="PK589_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19275.1"
                     /translation="MAKTGTRKAGEQAGGGRRMSSQAMKKHLRERLTADTQQVLKSDI
                     VSIFRKQGYYEELPLDELQDRLSKLQSPLADSLLKGRV"
     gene            complement(4714..5850)
                     /locus_tag="PK589_06455"
     CDS             complement(4714..5850)
                     /locus_tag="PK589_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HNJ19276.1"
                     /translation="MRDAEAMVSDRGTRISLVALVMLLCAGCGWVGGPSRPSWIDGSS
                     TQFPSTQYLVGVGQADSRPQATEQAYAAVSRIFKAEITAQAKDWESYLVVESRGQTST
                     QRKLILDNVTRVTTDKVLENVQVLDTWFDQKARQYYALAGMNRAQAESAMLERLADLD
                     RSIQTEVTEAHQAQDKLSHVRNLKRAAKNLVLREAYNTDLRTIRSNGQGHPAAYRVAE
                     LTAELEHYLAANMGMAVEVSGEQAEPLERALIEGLTQEGFSVVGSGPGAATAELLITG
                     TVRMWPIQVPDPQFRYVRWCTDAVIEEVSTHRVIGAVSKGGKEGHVTEREAAAKAVRV
                     MQQEFSADLAQAVAAHIYGERDLSVSSSAPSGCPKEAGQPPLSR"
     gene            complement(5847..7280)
                     /locus_tag="PK589_06460"
     CDS             complement(5847..7280)
                     /locus_tag="PK589_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19277.1"
                     /translation="MHSVIGWCLLWVLSGCGLSSNHYLLIDQSLAAHDARRADAIMAE
                     AESEYGPRNRLLYSMDRGMTLHLAGEYMQSNAQLESAAQEVERLYTRTIRTETAAFLT
                     NDNVLPYEGDPYEHVMINIIKALNYAAMGQLMEAVVEARQIDHRLNVLSDAAKEKDGY
                     REDAFARYLTGVLYEATGDLNNAFIAYRKAYDIYDATKSWARTPMPPMLRADLLRTTD
                     ALHMTTEFEEYRRLFPETRWLPRADQQNLAQVVVIGYNGRAPRKEDAFLDIPISLSAL
                     QLVLLNRGVIHASNRQERRVADSVLYGLNGRVVRVALPKLILQKTQVVHETVTLIPRG
                     GEPISATSELVYNGTALAERSLSDRLPGITTKALARAAVKFAAAEAATRGSQHAVNKG
                     DAPWVGLLVGVLAHGLAVASESADTRSWRTLPDEIQISRLWVPPGEYESRIQAMVRGN
                     GTPQVEGPRPLVLRAGQTLFLIERVML"
     gene            complement(7358..7987)
                     /locus_tag="PK589_06465"
     CDS             complement(7358..7987)
                     /locus_tag="PK589_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HNJ19278.1"
                     /translation="MIEWREHSVLRTCAVGLLVGCAAVVSGCGHETKVTRVDTGTVTD
                     LSGRWNDTDSQMVAEAMVKEALANPWLGNFTKGKNRQPVVIVGTVLNKSHEHINVQTF
                     VNDLERELTNSQKVTFVAAKGERDEVREERREQAVHAREDTQKAPGKEIGADYMMRGS
                     IATILDEQDGAKAVFYQIDLEMVDLENNVKSWFGQKKIKKVIEKKRTIF"
     gene            complement(8027..8593)
                     /locus_tag="PK589_06470"
     CDS             complement(8027..8593)
                     /locus_tag="PK589_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19279.1"
                     /translation="MSRVEGAFSKFTGSALMLVLLIGLTACGGPPNWVKKGSGAFNEK
                     SDKSFYGVGSVVGVRNEPLAWDTAENRSRAEIAKTFETYTAYLMRDYAASTTAGDFSR
                     NSEEQNIERAVKTFSAVTLNGVKPMDRYKDEKSGTYYVLTKLSLEDMKNNLEQAKELN
                     SQVRDFVRKNADRLFDRLEKEEDKRATK"
     gene            8830..9000
                     /locus_tag="PK589_06475"
     CDS             8830..9000
                     /locus_tag="PK589_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19280.1"
                     /translation="MADVQIEDGIIRVVQLDIQDPKAAAVLAEYPQVRWPEITRRALK
                     IGLGYLKGGGKD"
     gene            complement(9092..10471)
                     /locus_tag="PK589_06480"
     CDS             complement(9092..10471)
                     /locus_tag="PK589_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNJ19281.1"
                     /translation="MRSPRSLTFPRMITCLAALFVAGMSTDHAAAQVGKPEGLYYKSW
                     AVVVGIENYLVAPKVPGALESSHAVAEALRGLGFDEVIEIQDKEAASKRLLHILNDYL
                     PRKVGRQDRIVFFFTGHAGLTQDSQAKDSGFVVPWDAQLNNPAKAITLDQLKEFSRRS
                     ASKHILMVFDANLRGWDVTAPQPLSLEGRLSPEDDTEKRSVQVLTAAEKEESVVRTSG
                     QSAFVTALVAGLKGAADLNKNGWIMASELAAQVKQDVERETKGAQHPQLLQLEGDGDV
                     ILVEGRKAMFSVGKEPSTEADRMKEARVQYEQAFALLQQQKSAEEALERLNRAITYDP
                     SFGDAYVLKSFIRLEVLPNLEESLAAAQAAVQHAPQNPDSHYSLALALEKKGQHQEAE
                     RAMLQALAVNPAYTDVYFSLGTLYADHLNEPQKSVDAFRRYLELGGQSERATLAIQGD
                     RAPGEKPAP"
     gene            10700..12589
                     /gene="acs"
                     /locus_tag="PK589_06485"
     CDS             10700..12589
                     /gene="acs"
                     /locus_tag="PK589_06485"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNJ19282.1"
                     /translation="MSEKIDTLLKEGRVIQPSARIKAAAHIPDYDQAYKASIADPEAF
                     WGGVAKELEWFSPWSRVLEWNYPWAKWFVGATCNIAYNCLDRHTNGWRRNKVAIIWVG
                     ENGEERIFTYGELLRQVNRCANALKALGLNKGDRVTIYLPKIPEQVVAMLACARLGII
                     HSVVYSGFSAPALASRIQDAEARLVITADVGYDRGKTIPLKPVVDAAVQSCPSVEKVV
                     VVRRDALSVALTAPKEIDWADWLKEQNAVCAAEPLDAETPLYILYTSGTTGKPKGVVH
                     VHGGYMVGTYITTKYVFDLKEEDVYFCVADPGWVTGHSYIVYGPLLNGATILMAEGKP
                     DYPNPGRWWDLISRYGVSIFYTTPTAVRLLMKYGEEWPKKYDLSTLRILGSVGEPINP
                     EAWEWFYRVTGSDKPIMDTWWQTETGAILVTPLPSVPLKPGSATRPFLGIEADVVDKE
                     GNSLPANAGGFAVIKKPWPAMMRTIYKDPDRYKVYWNTIPNCYTAGDVCRKDQDGYMW
                     FMGRADDVIKVAGNRIGTAEVESALVSHEAVAEAAVIGKPHRTAGEAIKAFVILKQGY
                     QDSKELVQSLKDHVLKELGKIAVPQEIDIVSSLPKTRSGKIMRRVLKAKELGQDVGDI
                     STIED"
     gene            12642..13157
                     /locus_tag="PK589_06490"
     CDS             12642..13157
                     /locus_tag="PK589_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ19283.1"
                     /translation="MLGQRTTRGLLWSMVTGIILLGGVVPILAADDTPKAAGQAPAQA
                     KKAPEKKSVKKGEGRKTTTAPVKSPATKYASAETAAKAQACFGEAPKIESVKPDEGKA
                     GEKVTISGMNFGPAECLRSLSFGPGNAATFKLDSPTQITATVPASKRKGMAIMTVTTA
                     SGEDSKGFLLK"
     gene            complement(13374..14030)
                     /locus_tag="PK589_06495"
     CDS             complement(13374..14030)
                     /locus_tag="PK589_06495"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNJ19284.1"
                     /translation="MFSGIVEEMGAVSILNKGLAGTRLTIMASTIMTDLTIGASVSVN
                     GACLTAVARTDHDFSVDVSPETLSVTTLSDLTTGSPVNLERAMKLNERIGGHMVSGHV
                     DGIGAIRSRHQDGNALVLEIEAPKDILRLCVHKGSITVEGISLTINEVTERSFLVAII
                     PHTAKVTTLGLKQVGDKVNLESDLIGKYVERLLQDRGLLPPKPTPVIDKDYLQRRGLI
                     "
     gene            complement(14210..15385)
                     /locus_tag="PK589_06500"
     CDS             complement(14210..15385)
                     /locus_tag="PK589_06500"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ19285.1"
                     /translation="MTTSRSFFYLCTLGVFCFISYNLVRMPVLSLFAESLGAGPERIG
                     LIVSVSTITGVLLKLPSGALSDIYGRKMLLRIGVVAFGLPPFIYPFISDLNALTALRF
                     VHGLATAIFAPSALATVADLYKERRGAAMGTYTACTQSGSLLGPFIGGWLAHAAGFPA
                     AFVTAGVFGCIAILIFFSLHLDEPPPRVREKGLAPVITEMGKGFLAVARNRKVLITSS
                     TDAAKMIANGALMAFLPLYGLSVGLNAGEVGLMFSVQAVTSFLSKPVMGRISDRVGRQ
                     PLIVLGLVICAATFISIPHVGSFAVLLMLSSGFGFGEAVVSSSSAALVADSSEFKRLG
                     AGMGMQGTVMDIGHASGPLLAGFLIAHASYQGAFAVIAGIQILAAVAFWATMRTILR"
     gene            complement(15393..16184)
                     /locus_tag="PK589_06505"
     CDS             complement(15393..16184)
                     /locus_tag="PK589_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19286.1"
                     /translation="MVRRIGPLAAVLTAALALCDSTGQAEPERCSLPSHHVGMVFPLD
                     QVDAAWTCRLEPIIAHYTTANKVGPIRTLLPEDVYLYLLEHPVMAAALVNRLDLGLYK
                     AEWRGPDEFWATDGEGTEGIVHPLYQDRSTRIYYVEGTHDGRFLPRVSGKALVLFKLQ
                     PVKDGQGSESMDNTMVAYLRLDNRLYSGLLSMLRPLIGNVVTRQVVKAFDAARRLAGV
                     MRDHPDQVLFEATDPPGLPDEQVAFLKAALASLHNPGQKPSPALP"
     gene            complement(16199..17527)
                     /locus_tag="PK589_06510"
     CDS             complement(16199..17527)
                     /locus_tag="PK589_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19287.1"
                     /translation="MRSSLRPAYRVHPRRPGLLLSLVCIWLALLSVSLTHAAETASSS
                     DLAAQVWQYLTTQDSEQANSALASIMQRPDATLQAVQDILRKGQPHGLQPVGVLADEQ
                     VMVRDRPYRVSLSIPQSYEPTKDYALVVCLHGAGFTGEAYLDRWKSRLGEQYILACPT
                     YPAGAWFTRRAEDLVLATVQLVRERYRIDPNRMFLSGMSNGGIGAWLIGMHHAPLFAG
                     LAPMASGIDDVLFPFLENLRTTPVYIIHGSQDQVMPVELSRKLAGELKNLDYPYIYRE
                     HDRTHAMAGGHFFPREELPDLVKWFDTQHRTPVPKTVTVVRDASHLLPFGWVRIDATD
                     QIASFSEDLVDKRDDSIRKRIYARLTAQVTGPNRVDVRTDRVRQYTLYLNEDLVDLSK
                     PVVITTDGQPSFEGLLEPHVETLLRQARLRQDPAQLFPAHVTVTVPQRAS"
     gene            complement(17535..18662)
                     /gene="ribD"
                     /locus_tag="PK589_06515"
     CDS             complement(17535..18662)
                     /gene="ribD"
                     /locus_tag="PK589_06515"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNJ19288.1"
                     /translation="MKLSSRDREVMALALRLAAKGRGHTGPNPMVGAVVVNRGEIVGQ
                     GSHRKAGGPHAEVLALSQAGSRAKGGTLYVTLEPCSHLKKRTPPCVPLVISSGVRRVV
                     VAMVDPNPQVQGRGIAQLKRAGIQVEVGCLEPEARQLNETYIHWVQTGRPFTILKAGM
                     TLDGQIATATGESQWITDEAARMQAHQLRADVDAVLVGIGTVLRDNPQLTARTGGDPV
                     RLAARQPLRVVVDSRLRIPSRAAVLKRQEEAHTVIATTRAASVTKVERLRGQGIDVLV
                     LPAAGGHVNLTALWARLGQLGVTSVLVEGGSDLNAAVLRAGLPQRLMCFVAPLLLGGQ
                     DAKGVLGGLSPRRLREAVLLNNLRMTPVGRDMLIQADFPTR"
     gene            complement(18659..21508)
                     /gene="ppdK"
                     /locus_tag="PK589_06520"
     CDS             complement(18659..21508)
                     /gene="ppdK"
                     /locus_tag="PK589_06520"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNJ19289.1"
                     /translation="MAKKYVYYFGDGKAEGTGNMKELLGGKGAGLAEMTNLKVSVPPG
                     FTISTEACVEYYKRGKAYPPGMMEEALVALKKIERSMKAGFGDPDSPLLVSVRSGARA
                     SMPGMMDTVLNVGLTTKTVHGLALKTKNERFAQDSYRRFIGMFGSIVMGINREHFEDI
                     LKHKKRDLGVTQDTHLDAKALKELVVSFKELVKEETKRDFPDDPLEQLRMAINAVFSS
                     WYGARAVTYRRLYNIPETWGTAVNVVAMVFGNMGETSGTGVAFTRDPATGQRQFFGEC
                     LTNAQGEDVVAGIRTPLPVNQLEKFMPQAYKDLEVTYKKLERHYRDMLDLEFTIQEGK
                     LYMLQTRVGKRTGVAAVRIAVDMVKEGLISKKEALQRIGPDQLAQYLYPIFDAKEESH
                     CTPLGKGLPAGPGAAAGKIALTADRAVEMQAAGNRVVLVRQETSPDDIHGMNAALGFL
                     TARGGMTSHAAVVARQMGKVCVAGCEAIEVVDNQTVRIGTQTFREGDYLSVNGSTGNV
                     YGGDIPVVESEVIQVLQGKMEATASEKYQLFESVLKWADGVRKLKVRANADVPDQARI
                     ARSFGAEGIGLCRTEHMFFAEDRIQIMQKMILARKREEREMYLDQLLPLQKQDFIGLY
                     REMKGFPVTIRLLDPPLHEFLPKREDLMVEIAQLELTSGSQTVLEEKKRLLTRVEELH
                     EFNPMLGLRGCRLGITMPEITKMQARAIIEAACELAKEGTKIVPEIMIPLVGMVSEMK
                     AQKDLVREVAAETMKRYGVKLSYLVGTMIELPRAAVTADGIAEEAEFFSFGTNDLTQT
                     TFGFSRDDAAKFIDFYKTANILESDPFAVLDREGVGSLMRTAITGGRKTRPTIKLGIC
                     GEHGGDPSSVEFCHQLGLDYVSCSPYRVGIARLAAAQAALAEAEKEKPKPAKSKPKAA
                     KPSAVKSVKKSAKSAPAKKRPVAKRVVRRSKRTSR"
     gene            complement(21534..21947)
                     /locus_tag="PK589_06525"
     CDS             complement(21534..21947)
                     /locus_tag="PK589_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19290.1"
                     /translation="MMVVCNAVFLFGIWVTGLNFERLVRTPDIYDSRQDICLRLAYQH
                     VPGSENPVQFCSEWLNLADPTGKTHTFQKDAQLKQGADGSFYFDYGPFVDYRLFAVGA
                     FVVGIIVCGIRVTRHVVNRYRMRLEAAARHSVPTH"
     gene            complement(22067..24268)
                     /gene="glyS"
                     /locus_tag="PK589_06530"
     CDS             complement(22067..24268)
                     /gene="glyS"
                     /locus_tag="PK589_06530"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNJ19291.1"
                     /translation="MPSKKPKTKTVSRAKKPASASRAKTPSTTELLLEIGTEELPYQF
                     VAPALRALQQGAETLLKDSRLTYGTVRTMGTPRRLVLLVEGLARQQASAVKEAMGPSK
                     AVAFDQSGQPTRAAIGFAAGQGVPVESLQVRQTPKGDYLFAVKPEKGQAVAAVLTQAL
                     PQLLAKLSFPKAMHWNQTGVRFARPVRWLIALCGGKVLPIQFATIKAGNYSQGHRVLG
                     AKVSSPKGFAVQSIAHYFKETERHSVIVDQDRRRAMILDQLAALAKSARGHLHQDEEL
                     LEQAVYMVECPLTILGSFKPHYLALPKEILMTSMKEHQGYFSLVDSKGALLPNFLAVT
                     NMKLANMQLIREGNERVLAARLADAKFFFDEDRKTSLADRVAKQLAVTFHQKLGSLHQ
                     KTQRVVAMAAHLAGQLGDDQLVHDCRRAAELSKADLLTGIVGEFPTLQGMMGGEYAKH
                     DGESATVSAAIREQYLPRAMEGELPESLAGKVLSLADRLDSIAGFFLVGLVPSGSEDP
                     FALRRHATAIVRILIESRLQINLAQAVRATQEGLAGQHVAAAPQPAKGGAPDVIGFLF
                     ERVRFYGKSAHQLRDDVMEAVLKSADRQSIDLTDLFDKMQSLQQITSRAEFDPLIVGF
                     KRAHRLTEKEQWDRKPVESGLFREPAESALYETVQQSHAAYAAAMRGGQYGQALDVLV
                     RMKGPIDDFFNAVMVNADDPAVRGNRLSLLKEVDDLFMSFADFSQIMVQGT"
     gene            complement(24290..25210)
                     /locus_tag="PK589_06535"
     CDS             complement(24290..25210)
                     /locus_tag="PK589_06535"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNJ19292.1"
                     /translation="MNFQDLILTLHRFWADRGCVIHQPYDLEVGAGTFHPATFLRSLG
                     PEPWSAAYPQACRRPTDGRYGENPNRMQHYYQYQVVLKPAPDNIQGLYLESLKQLGID
                     PKKHDIRFVQDDWESPTLGAWGLGWEVRLDGMEITQFTYFQEIGGIPLNPITGEITYG
                     TERIAMYLQQVDNVYDLQWSDGVTYGDVHHRSEVEFSRYNFEEGEVPMLMATFQAFEG
                     ECQRLLDKKLTLPAYDYCIKTSHMFNLLDARGAISVTERTSYIARVRALARRCAESYV
                     ADREAMGHPLIKQPAGAGKGTINHSPVTTK"
     gene            25455..25895
                     /locus_tag="PK589_06540"
     CDS             25455..25895
                     /locus_tag="PK589_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNJ19293.1"
                     /translation="MKFSKKSEYGLRALLELCETYGGRVLQRHEIAERQNIPVEFLEQ
                     ILLALKRAGLLASRRGIRGGYSLIKSPEEITLGQVIRILDGPLAPISCVSKTAYQKCS
                     DCPYAAKPSCPLQQAMGEVRDAIANILDHYTLSRFAHTKLAEGS"
     gene            25895..26959
                     /locus_tag="PK589_06545"
     CDS             25895..26959
                     /locus_tag="PK589_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNJ19294.1"
                     /translation="MRTITHTFSTLMLSVIASWGLFTFPVAPSHAAETRDLILAAYSV
                     PKEAYERHIIPAFQRYWKQKTGQDVRVRSSYGASGAQARAIIGGFDADVAVLSLEGDV
                     DQITKAGLITHDWRKAPHGGMISASVVALGVRKGNPKGVKGWEDAARPGVEVLYPNPK
                     TSGGAMWDVIAIYGAGLKLAEQRAGKPVAPEAAATQAVDLLTRIQRNVKVMDKSGRES
                     VTTFERGVGDVIVTYENELLPRVKSGRPYELIVPAETVWIENPAAVVDTYADRHKAKD
                     LAEAFVAFLHGPEAQAAFIELGFRPLDRGAVAPASGAALPQPARLFTIADLGGWESVS
                     TKLFGAQGVWTKIVEDVSKK"
     gene            27221..28102
                     /gene="cysT"
                     /locus_tag="PK589_06550"
     CDS             27221..28102
                     /gene="cysT"
                     /locus_tag="PK589_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNJ19295.1"
                     /translation="MTSHMLLRVALRSAAVGYVLLLIFLPLATLTQQSIASGWDRFIQ
                     DLSAPQAAAALWLTIETSAIATAINAVFGTLSALVLVRYEFPGRWLLNALVDLPFAIP
                     TLVAGLMIAAIYGPTSVLGTWLQQGGLTVLYNQPGIILAMLFVTMPFTIRSLQPVLMG
                     LERDQEEAAFTLGASPWTTFWKVTVPSVLPGLLTGVFLTFVRALGEFGSIVIVAGNIP
                     MKTQVASVYVYGEIESYNPQAATSVSVLILLISFVVLLLLERLTRRPGERLPKLFRWA
                     NRHDQPGRDAAAATAAH"
     gene            28151..28960
                     /locus_tag="PK589_06555"
     CDS             28151..28960
                     /locus_tag="PK589_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HNJ19296.1"
                     /translation="MRWLLIGIVWLYFLVLLVGPILYMASQSFSEGLAAFWTEISRPE
                     AVHGFTLTAEITLIVLVLNLIFGTLTALVLARQQFWGRSLVSGVIDLPFAVSPVIAGF
                     MLILLFGPDTMLGALFGQAHIKILFALPAMILATLFVTFPFMVRELTPLLQTLGTEEE
                     EAARTLGAGEWQVFLKVTLPALRWGLVYGATLTVARAIGEFGAVLVVSGNILLLTQTA
                     TLHIYQSYVDFNYVAANAVALTLLAVSFAILTVLEIAKARAETTVAEAGAQ"
     gene            28965..30062
                     /locus_tag="PK589_06560"
     CDS             28965..30062
                     /locus_tag="PK589_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ19297.1"
                     /translation="MKIEVRGLTKKFGSGTAVGNVSFVVHEGELLGLLGPSGSGKTTV
                     LRLIAGLEVPTSGDIFIDGKRVNDLTVQERNIGFVFQHYALFKHLTVFDNIAFGLKIK
                     KWNRQDIEQRVLELLSLMSLDGLGSRYPHQLSGGQRQRVAIARALAPRPSVLLMDEPF
                     GAVDAKVRQELREWLIRLHTDLNVTSLFVTHDQEEAMEVSGRIIVFSKGKLEQAGSPA
                     DVYEEPATEFVARFIGSMNIVEGIVRRNQVQVGSLEFPAPGFSDGQKLQVGFRPYYVK
                     VAEDATRYRQQAKLRHIYFLGVAYRLEIETADGLILRSRMNKEEFRRCNFTVGQAVSF
                     AVTHFRILPQEGAEASSEAKPGSPITGPLTP"
     gene            complement(30122..30499)
                     /locus_tag="PK589_06565"
     CDS             complement(30122..30499)
                     /locus_tag="PK589_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19298.1"
                     /translation="MRKLTPLHGDFLAYCVDDWTGLWLLVKRIKPLHSSTDADKIQAH
                     ALEIIRDLIEAGYIMAGDVDFGLGKFRQWNLSVDKIIGRIEQEWIELGRDPTICEIVW
                     FVSTTEGDYAFASGQFPETPSTE"
     gene            complement(30671..32140)
                     /locus_tag="PK589_06570"
     CDS             complement(30671..32140)
                     /locus_tag="PK589_06570"
                     /inference="COORDINATES: protein motif:HMM:NF040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HNJ19299.1"
                     /translation="MVRYSNLWQCLVTVLLLCGAGQVECWAGDTPHASHAHGPLRQSP
                     DNPRYFADADNRIVYLTGSHTWENFQDVTQVFDYPAYLDRLTALHHNFMRLWVTESIH
                     SDGGWLMNPPPNEKDIAPLPYLRTGPGLALDGGPRLDLSRFNPAYFERLRARVKDAGQ
                     RGIYVSVMFFQGFSVVTPGGWKAHPLNGQNNVNGIDGDANGDELGLEVHRSPGVPVLQ
                     IQEAYVRHVLETLHDLDNVLYEVVNEATPESVSWQYHFISFIKRYERERGFMAHPVGM
                     TFFQLGEFGGGDNRTLFESEADWISPGGYTKYARNPPATDGRQVQVLDTDHIHGIGGD
                     QQYVWESFMRGYNPIYMDPFDAVHELTIGEPVLNEPQHERARVAMGQTRRWADRINLK
                     RVTPQSELASTGYCLADRGREYLIYIPASDSVAESGAEKPTRPVTVNLTGASGVFTAE
                     WMELETGRTKQADSLIGGSERMLTVPFPGSGLLYLRKSG"
     gene            complement(32257..33135)
                     /locus_tag="PK589_06575"
     CDS             complement(32257..33135)
                     /locus_tag="PK589_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19300.1"
                     /translation="MISPPILFFCLFTLVMAGAPPPALSAQLTAREEDGLIGTVQSVE
                     TIDSLVSQTDYYDPQGRILERIQGGKDTSHGLWPLRFTYVYDKEGRRIAEMIRDAQGE
                     LVKETRAVYDAQGNRSAELSAWGDGTFENVSLYDYDELRRLRRGLHYNAVHVVNRNLY
                     SYDGGGRLLQERFERNYRYDADGHQMVTTDRFDVGHDVAIIYNDRGQVQQKTVSDLRG
                     RRQGHSEFRYDERGSQIEERSYDAAGRLMDRKTYRYEYDTVGNWITETFQWWELRDGE
                     PRIKQSHVRERRITYH"
     gene            complement(33218..33997)
                     /locus_tag="PK589_06580"
     CDS             complement(33218..33997)
                     /locus_tag="PK589_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNJ19301.1"
                     /translation="MLLEGKKGLIIGVANKHSIAWAIAQAAAREGAQMLFSYQNERLR
                     ENVEELVQTLPGASACVCDVGDDSQIAAMMKEAAEKLGRLDFVVHSLAFAPREELTGE
                     FVNTTRQGFATALDVSAYSLVAVTRAAMPLMTDGGSVVTLTYLGSERVVPHYNVMGVA
                     KAALEATVRYLAHDLGPKNIRVNAVSAGPIKTLAARGVSGISKMVDHHRAFAPLRRAT
                     EQGEVGDTALFLVSPLGRGITGEVIYVDGGYHILGSLASVD"
     gene            complement(34250..35116)
                     /locus_tag="PK589_06585"
     CDS             complement(34250..35116)
                     /locus_tag="PK589_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19302.1"
                     /translation="MAEVVGLGLRIFGVLLCAALALATTGEAAGKVSGTLILHDALTL
                     PNHSVRIEAQVAVTAAGPPLSGLMLHLQIDGKDVATAKTNEGGRAAFDYLAKMRGTNV
                     ISVSIDPDAPVAAEKVDATLCVWERRRPIVVVELDALIQQSPAPATPAASPGIGVTDD
                     TVPTPVPEAAEELSRLTQYYYNVVYVSNNEQGPMGNVSSGQVRRWLEAHQFPVGFVAT
                     PTSGGLNATLDALKHDGWTTMKSGIGRTRGFADMLLQHRMEVVVVPELPKGELPRKAK
                     AAKDWKEVRKKL"
     gene            complement(35156..35344)
                     /locus_tag="PK589_06590"
     CDS             complement(35156..35344)
                     /locus_tag="PK589_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19303.1"
                     /translation="MPGLGHLYIGEKRGGPLLAAGLLLIILWTNWFAFALVPLLGLVL
                     FSMGDTYLTVSRDRGLFS"
     gene            complement(35448..35915)
                     /gene="bcp"
                     /locus_tag="PK589_06595"
     CDS             complement(35448..35915)
                     /gene="bcp"
                     /locus_tag="PK589_06595"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNJ19304.1"
                     /translation="MASGVEVGAKAPEFSLPDQDGNLVTLKSLKGKQVVLYFYPKDDT
                     SGCTKEACDFRDSLAAIKKTGAVVIGVSKDGKTSHQKFIAKYGLPFALLSDEDTAVST
                     AYDVYKEKSMYGRKYMGIERSTFVIDATGRIKASFRKVKVPGHVEEVLAALKA"
     gene            complement(35950..36390)
                     /locus_tag="PK589_06600"
     CDS             complement(35950..36390)
                     /locus_tag="PK589_06600"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ19305.1"
                     /translation="MRVSRWIALVVVVLSCSACAHEKPKPLVPLELAYGAKADANALN
                     NQGRQAFLAGQMAEAKDFFGQVIKIAPDSGQAHYNYGLTLNALGQTDQARQEFIEAAN
                     LAPGDKVIWDSPALRPFGNPEVPKGPAREHPYGTSRPTIGSGPR"
     gene            complement(36425..36700)
                     /locus_tag="PK589_06605"
     CDS             complement(36425..36700)
                     /locus_tag="PK589_06605"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HNJ19306.1"
                     /translation="MISDDKASHLAHLILGTLKKSPQCRLTEDDGKVLREIKRVVAAE
                     LALDAEIDKKVRAKLASYTKQIVEGSPEWDVMYRKTSEEEQKKRVKP"
     gene            complement(36734..37015)
                     /locus_tag="PK589_06610"
     CDS             complement(36734..37015)
                     /locus_tag="PK589_06610"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HNJ19307.1"
                     /translation="MRLAKERVHHMADSVVARLQELNFLEVTGDRKALREVLEHTMTE
                     ELMVEDRLNAEVRRMMQPYERQIEQGQVDYQKMFTMIKQKLVRERGIIL"
     gene            complement(37051..37461)
                     /locus_tag="PK589_06615"
     CDS             complement(37051..37461)
                     /locus_tag="PK589_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HNJ19308.1"
                     /translation="MTTNGTMKRIRIKVGGLQLEAELKGTRTAEELYRALPAEGPLNV
                     WGEEFYFKIPGVKDHRETATTQVKIGDVAFWGAGQVLAIFFGRTPMSMGADPVPADRV
                     NVVGRVLGDAATLRQAMNASMIRVERIEPTQPVG"
     gene            37578..38351
                     /locus_tag="PK589_06620"
     CDS             37578..38351
                     /locus_tag="PK589_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNJ19309.1"
                     /translation="MLTSTFVHLKGIGPSTERRLWESGIADWATFRREAHLTGIAPAR
                     KAAYDAELALAQDHLDLHNPRYFADCLHTRDHWRLFQTFGARALYLDIETTGLSAREG
                     QVTIVGLHRQGRMRTLIHGQDLTLEAIQEELDQADLLITFFGTVFDIPYLQTCFHGLR
                     VAIPHFDLCFAARRVGLQGGLKSIERELDIARESDLLQLDGLEAVRLWHHYRAGHDEA
                     LDQLVRYNAADTQNLEPLAACLYERLVLRYGPQSLGLVG"
     gene            38353..39159
                     /locus_tag="PK589_06625"
     CDS             38353..39159
                     /locus_tag="PK589_06625"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNJ19310.1"
                     /translation="MALAQGKWDWTGYGQTDRGLVRSTNQDAFLVDNRRRLWAVADGM
                     GGHPGGDVASRIVMDTLADFSPSLIRNSDNLDEDHTEQQSFRLTTMAEQAHDAVRAYA
                     DRHPSVYGMGTTLVMAHLMPLPAPRLLVLNVGDSRAYLVRAGRITQLTRDHTLIDDYV
                     REGRLTPEQAARHPDRHVLTRAIGLEPVLQIDLFPVDVFVGDVLLLCSDGLTKMLPDE
                     HILKIAQSHRDSPSLTTQALIQAAIDTGGIDNITVVACAMTSLPDQGNIH"
     gene            39246..40151
                     /locus_tag="PK589_06630"
     CDS             39246..40151
                     /locus_tag="PK589_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ19311.1"
                     /translation="MESHYFRPLSLRVRTWLLLGPLLGMLYLPTARAGEPHDAETTLR
                     RLVQANAERDLATMSKYMSHDVDAIGYTIDGRKYIGWSDLAADMRSEFDSVVKLEIPI
                     TDLRMWNNKDVAWFAMELDYIRYSDPADLAQRMVLHLRETGVLEQRNGRWILVAWHES
                     TRHHDSGTEQTAANGAALLHRASDAQPAVPNGVDLSGEWEVTEIEDNKKYVATLNAQG
                     TGPYTWQGGQFSTTSFNDRRWQGTWKQTGNDREGAFEVVLSEDGTEAKGIWWYVRVGT
                     RDNIPPRQHGGSYLWKRLTPPPTAP"
     gene            40227..41228
                     /locus_tag="PK589_06635"
     CDS             40227..41228
                     /locus_tag="PK589_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19312.1"
                     /translation="MNRALLLVVMFLGVTSLAYADGGHDHHAIPTMPSLGETKLTIDE
                     QTVTLTFGPVDLPTGHDGDLASSMPKKVFQLPEDTYMIGYKSEVFTKDGKPLPKNYLH
                     HILMLNNDKPSVSCDGEPLFFAGAGLEMTEARFPDGYGVKLGKGQNLMSVVAFYHKAP
                     PTKDVMARFTMYVAPKGAKVQEMDVYQVGVNIVCYSKFAQRGPDQTDEGIEIKSGVSV
                     LSAPLKFNMDGCVKFAYPHGHDELLMVALENKTSKETLLRTVPEVDADGTFRDFRPHQ
                     VYRDSKGFSVTKQDDYEMVMVHHHLLQNPNIQHGMGNYLLYMTPGACQQSSTTAAAH"
     gene            41608..45789
                     /locus_tag="PK589_06640"
     CDS             41608..45789
                     /locus_tag="PK589_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ19313.1"
                     /translation="MSLPEPSLHILLINENADEIKLVTTSLRSFFVGCRIEAGYSSED
                     ALAFTRQGDWQIILIDQDLSPERGLDLLARLRRNAPYAAILLQTNESDSHTAVQALQH
                     GADFLLFKRSPGFLTELLFSVQEALEKRDLQMKLDHTFQRHLRVIETLSDLLYELDAD
                     GRFVYVSPTVTAMLGYTAEELAGRHYSLLLPPQQEPTARFRLNERRAGSRSTRRVELT
                     LYRKALPEAPSVPIIVEVTAKGLFDSTNRYLGTVGLLRDLSQQKAQQNRLAELESRLQ
                     ETDRQLTLSREAARVSRQLQQPLTSLLQDSQRLLTSIQHSRFEQHVETMVAQASQASQ
                     LGHQLAEAIHAPPTELILLDLNEVLQTVAQTTHGETEDSQFLLTTQFAANLPLILGSR
                     ADLEDLTHILLSYACRSTPDNTRPSLTLHTAVLAVETSGSPGQEVASLSRPLRTYASF
                     TIRGSASSLRLPPDSQSGHDRSAVSLFQAHRIVQVHRGAIEVEHTPESGLAITVRIPA
                     SEDFSLTGTPATTISPPPTDIHKPGRDTSQRPERRRSERKPFSLPVQLSIGGTTLRGL
                     LRNMSTEGALLTIRGMESSIHLQPAYVVIKTPVSFLELQGVVHERPSASTELSLPAIK
                     DFVISFALAVEHERNVLRSLLEGLQEGSPQITFEGLILPLPIAQTIQHRNLLPLREMP
                     EDRREAARLSVSCPVYITGSDAASSRPFGKIINLNLNGACIELPTEVVLTEHSRVIHL
                     NPLESITMVPGTSQAPEAEQPLTAQILWNDSAERRLKLRLAATGHHLQQIGIRFDTLS
                     PAQEQRLRSIMEAEFRAEQHLAETPSDAPVLTVSHTVRNREGYTIALRHDFPTGGGSE
                     HDPLVLLCPGYGTTQHAYVALAYMLAGRGFHVVRYDHSRHIGLSDGDPSQTTFTSLED
                     DLDTVLTFVQEHWPERPLTILAPDLPARIALRRQDWHRRIRRLLLLNPTLDLGQCLQT
                     LHHRDLLQEHLNGTCLGLGNLLGIPLDIDHFLADAVAAQYTDVRVLHNELAHCETEVV
                     VLTGGQDGPDCSIPPPPQPLVNDLLSRLGVKGRKVLLPSSTLTSGPQPPSTLRASWQQ
                     VSHLCQAGQALHEPGRVMLPSLLLATSTRARFERDQLRHKHAIGTTSNERLWTAHTEL
                     TRALDELPAHWQSIDQLYQLLQPLDGNVALLDIGCGMHSFARLLLLNLSYRLRAQTWH
                     HNHPLRYVGLDFSSASLHAARASTKEALQHLDELFSGRISAPTPVTQQWILGRSADAL
                     PFADCSFDRVVANFSLSFVRSPVHSLREFFRVLRPGGKLILKAFTPATDLARLYRPAL
                     HEIGIDAFSGAPRLALHRMGQCAVALRTGQLHTFEEESLTTLIARFTPLTPRLVRTLA
                     GQILLAAAEKPDSSS"
     gene            46025..47179
                     /locus_tag="PK589_06645"
     CDS             46025..47179
                     /locus_tag="PK589_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ19314.1"
                     /translation="MTALSQYTRLPEVALWIRESTGERYRLLASYGHNHPSSLPSTLT
                     GDHPLVAGLSAKPYVCHGSENPSKPLGLAMATARASLCLGLRTKGRLLGICILGPLDT
                     KAQVGEELLAIVDAITHIAGNSLDHYLTQDDLRRSSTLMRRTDRLRSLEIMAGGFAHE
                     IRNPLTSIKTFVQLAPQRQQDPVFIKEFSKLAIEDIHRIERLLHEILDYASYMTPRPT
                     EVDLNDLVASCIGFISSSAAHRGIQVQTHLSPQLPLLLLDRQQIKQVLLNLLLNALDA
                     MPNGNGIIRVHTRLLSEAGRPPWVQLQVEDEGCGIAPDHLEHIFDPFFSTKHSNSASE
                     GAGLGLTTAHQIVREHGGTLIVESRLGAGSTFSVNLPVPDSAPTVSAARE"
     gene            47181..48596
                     /locus_tag="PK589_06650"
     CDS             47181..48596
                     /locus_tag="PK589_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ19315.1"
                     /translation="MKKRVLLVDDEPRVRASLRTVLEPTYEIFEAADAAEGLRSFKHD
                     APDLVLLDVILPGADGLSALQTMRTENRAVPVIMLTGTKAVKTAVDAMKLGAADYLSK
                     PFDVEELQIVIERTLGKQELEQEVRQLRAQVVQRYAFHNLIGKSPAMQEIYAKIEQVA
                     DSRTTVLVTGESGTGKELVAKAIHYNSARRERPFVALNCAALPETLIESELFGHEKGS
                     FTDATARRVGQFELAHTGTLFLDEIGDLSPATQAKLLRVLQEREFTRVGGVQSIKVDV
                     RIVAATNKNLDEMVRKNQFREDLYYRINVIALYLPPLRERGEDIALLAKHFLSKRIEE
                     EKRPSQEFTKDALELISHYPWPGNVREMENIIEQAFIWSKGSTVITPEHLPNILRTDT
                     RSSSLRDDTLAGRLSLEKAVMEFEREIILDALKRTTYVQTHAATMLGISRRMLKYRMD
                     TLGISRPDSGGGNAEASLPQE"
     gene            48783..49925
                     /locus_tag="PK589_06655"
     CDS             48783..49925
                     /locus_tag="PK589_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ19316.1"
                     /translation="MGTQASTEQISIKPSVLVVDDETGPRDALKVILRPFFTIHSADN
                     AKSALRVLKDQHIDLITLDQKLPDRQGIDLLQDIKQDYADIEVIIITGYGSLKSAMEG
                     IRHGAAGYLLKPFNVTELITLINQTLEKKQRLDYLRSFLKTSTALWGTEQEAAEAWKD
                     LQSKYFAIGNSSTDATGTAADVTALIPLLSDLLEAKDRQLLNHCSRVSFYASLLANQL
                     TLPLPDQKALALGAFLHDIGKISLSNYKYSADDDESLKTNTHGKECFETGARMLLPLG
                     FQAEVGQIVAYHQERFDGVGNPHGLEGEGIPLLARIVAIAQAFDNLTVDMPGHQPTSI
                     DEAIRQILLQADTYFDPKLTELFAHVVRECKSSLPALAIAKTSPTN"
     gene            complement(49933..51618)
                     /gene="recN"
                     /locus_tag="PK589_06660"
     CDS             complement(49933..51618)
                     /gene="recN"
                     /locus_tag="PK589_06660"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNJ19317.1"
                     /translation="MLTELRISNFGVIEQLAVTFGAGFIVFTGETGAGKSLLIDAVTL
                     LVGGRASAEHIRAQAEEAELEAAFHLPPEHPLLALLRNKGFARSGETDILIRRIISRT
                     GRNRTYLNGNLCPVHLLEELGGALVDVHGQHEQQSLLSPSAQMDALDAFGGFQPLRQE
                     YQHAYRDWQDRVGDLETLTAQIAQRREREDLLRFQFQEMTDAGIEAGEDARLEQERPR
                     LMHSQQLGELSDQLHELLYAGDQGVLSLLAGARKLLAKMESLDATTVEWIKTMDEAVV
                     PLKDLADQVRRYRDHVEANPSRLLEIEQRLDRLHRLAKKYGGSLDAMLAWQDSLRAQL
                     AELDHAETHLQERTRAVEQAVLSLRDVADRLSRKRLDAAKKITAQVMKELGGLRMERT
                     RFSVEVSRLSGDAPYGQTGQDTVEFMFSANPGEPLKPLAKVASGGELSRVMLALKTIL
                     AESDQVPVLIFDEVDAGVGGAVAEVMGVRLRSLSRHHQVLCVTHLPQVGSQAHAHYLV
                     EKWVRQNRTMTQVRLLDLDEREEEVARMLAGVTVTKKARAAAAEMIASAKDRQ"
     gene            complement(51637..51984)
                     /gene="trxA"
                     /locus_tag="PK589_06665"
     CDS             complement(51637..51984)
                     /gene="trxA"
                     /locus_tag="PK589_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNJ19318.1"
                     /translation="MAGDALKATDATWDEDVMKASELVMVDFWAVWCGPCQMVAPIVD
                     ELATEYAGKVKVRKLNTDENPEIAGRYQVMSIPTILFFKNGQAVERLVGARPKRQFKE
                     IIDTLLAQHSGAA"
     gene            complement(52087..52653)
                     /locus_tag="PK589_06670"
     CDS             complement(52087..52653)
                     /locus_tag="PK589_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19319.1"
                     /translation="MIETKRAAGIRLNRRTVLTTCLLMLAIAVQEIYPSGLSATEHMA
                     HLERAAVFLKAGDYRQALEACQAEVKDRPSARSYLYLTYAYHALDGYLQYLANTDQWV
                     RVEHLYVNLSTGRVEDLLDPPDVLARIAKEVIQQAAQRQADVTAALAGRLDETLVKQL
                     WQQQTAWRQARPDDWWSAAPPQWQWAAP"
     gene            complement(52696..55182)
                     /gene="lptD"
                     /locus_tag="PK589_06675"
     CDS             complement(52696..55182)
                     /gene="lptD"
                     /locus_tag="PK589_06675"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HNJ19320.1"
                     /translation="MGRDGYSVSTWQRLISHFPCILSISILFGLFPLFAPSAWGESTV
                     TTVSPTNQPLTVTAERIDHLQEREVYEADGSVVVTQGNLRLTADHVTINALPGTLTAT
                     GRVHFWDASSDLKSERLELNVNTEAGIVANGSMFIRPSNSFFTGRSIRRLSEDHYRIK
                     EGTFTNCDAKEGEVPAWRFTFEDLDVNAGESIGMKRAWLCANDTPIIPVPTLTYPLSN
                     RHSGFLIPTPGYDNRFGLHYQQGYFWAINPSQDLTIAPSYYNNLGYGSDFTYRYYLDR
                     RSSGQWFASFLQQTKLPNVSGVDQVSSDERRLRGLISGQHVQQVTDTLLVRGQASFVS
                     DRQYLQQLSNSGTQRASPSGESTLLATQRLPYGSAYFLGQYLQPLNSGGPDTFQRLPE
                     VGYVLPNTSPFGMPVLLNLDTNFVNFYREQGFAVNRMDFMPGVTTEVMDVGHVIGLTP
                     HVKFKEVYYTRGIQEASPLHRETFWAAMDATSKLSRRYTGGEGGSFLHTIEPSVIYEY
                     VPGSDQSKIAQIDQIDDIPKKNLLTYALRTKFLEQQLNGQSFNWLDLTVAQSYHVGAV
                     QTRAREFTPGVLPFLGSLTQPLQPATVDVQGRKMSDVWMRAVIGNTAPEFFRPPAINE
                     ALGKNVTGGQMLPPTNSYLQVDAFLDPYRGTFSQWNTDLRVQQSNYWYVEVGQRFSHD
                     GNRVRRGDIWNPVSFNEVYAPTKEIQFATAGGGFRTPWGWTIGAKGYYDVKGGRSPEF
                     DVVALYQNPCKCWSLGLYYLQFPDRQSYSFMFSMTGIGWTENFGTIVARTILGPLLVG
                     DRGLPWAAPGGPYGRVQNALPVQSMPMGRY"
     gene            complement(55184..56506)
                     /locus_tag="PK589_06680"
     CDS             complement(55184..56506)
                     /locus_tag="PK589_06680"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNJ19321.1"
                     /translation="MQCESPVAPMTYPATVQFLYGLQLHGIKLGLETIRTLLARVGDP
                     HRQYPVLHIGGTNGKGSTAAMTASILQVSGHRVGLYTSPHLIDFRERIRVNGEMIPES
                     RVIHGVELLRAASAPDLAPTFFEFTTALAFHHFAECQVDVVVLEVGLGGRFDATNVVE
                     PLAAAITTIGLDHEAYLGSTIEAIAMEKAGIIKPGVPVVLGRIGEPGSRVIEERASTV
                     GAPVLRLDRDFRCEGSSPADCRYTGFGLRLEHLSCPLQGRFQIDNVACSLALIELAHE
                     RGVTVTETSVRAGLQQVAWEGRLEIVGQEPTVMVDGAHNPAAAAVLAEYLSEWKRVSP
                     AARLCLIVGMMRDKHPREFLAPLLPLIDTLILTQADLPRAATGSELRLLLEHDAPFAQ
                     VATTPSDALAMAKHSAAASDVVCVTGSLMLVGEIKALLRGDSLSPVRG"
     gene            complement(56647..>57170)
                     /locus_tag="PK589_06685"
     CDS             complement(56647..>57170)
                     /locus_tag="PK589_06685"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HNJ19322.1"
                     /translation="KDAMVIGQGVINGRKVALCVFDFGFMGGSMGSVVGEKICRAVDR
                     ALEARMPLILVTASGGARMQEGILSLMQMAKTSAAVAKLGEAKLPFISILADPTFGGV
                     TASIAMLGDVIIAEPKALIGFAGPRVIEQTIKQQLPDQFQRAEFLLDHGMIDMIVERK
                     QLKEAVSTLVGHF"
BASE COUNT        12142 a        17491 c        15699 g        11738 t
ORIGIN      
        1 caactctccg ccatctcacg accacggatc aaagcatggg ccgcagggct gcttgaaaag
       61 gggctggatt tcgatacggc actcaatgcg ctgctcacgc tcagcgcggt attgaccgaa
      121 gctgtggagg atgggcttat cactcacaac ccggccctcc gttccggcaa actcctgaaa
      181 cggccggcga ccatcgaaga atcagaactg gcgatcttca cgccggaaga ggaaggcctg
      241 ttactggaaa ccattcgacg cgatcgcccg ctgttttacc caatggccct gaccttcttt
      301 cgcaccggcc tacgagcagg agaggtactt ggactccatc ggggcgatct gaactttcac
      361 agccgatcca tctacgtgca acgtaactgg tcgcgcggaa tcctgggcac acccaagaac
      421 gggaaggccc ggcgggtgga catgtcgcag ggtctggcaa aggctctgac ggaatggatc
      481 gagctacagg acctggaggc ggccgccgct ggccacgcag cacctgaagt cctctttcca
      541 ggcaacctgg gcggaaccag gcgggagccc agttatatgg cggagaattg gttacggtac
      601 aaactgtggt ttccgatggt ggagaaagcg ggactacgac gactcgatat gcatagtgtc
      661 cgacatacct atgcaagccg actcatcgcg aatggcgaga acctgaaata tgtccaagag
      721 caacttggcc atgcgtcgat taccatcacc tgtgacacct atgggcatct aattccaggc
      781 gggaatcggc aagcggtcga taagctggat tcgttaaaaa tcggttctgg aaccgtgacc
      841 aaaaccgtga ccaacgccta cgaaaactcc taagtctttg atttttgagc gaatacagtg
      901 gcatcacaag cgatgtactt aatctgcttc atcggcacaa tgatgacttc cttggccgaa
      961 tccacctcaa gcacttccat gtcctcactg atggtatgcc ggatggcgtc cttgaccagc
     1021 agttcctgat tatcggcaaa tacgactttc acccaatatt gcacgagacg cgctccttgt
     1081 tagagatgat ggttctcggt cttgcccaat tcctgcgagc gttgcgtggc cgcttcaacg
     1141 gccgacatca gggtggcccg cagctttctc tgttccaact gatacagtcc ggcaatcgtg
     1201 gtcccgccag gcgaagccac ccggtctttc agtacagcag gatgctcgcc ctgctccagc
     1261 accatgctgg ccgcgccggc cacggtgtgc gctgccaaca gttgtgcggt ggatcgcggc
     1321 agacccccca acaccccgcc gtcagcaagc gcttcaatca tggcgaacac gtaggctggt
     1381 ccgctgccgc tcaaggccgt cacagcgtct aacagccgtt cctctacgac gacagccata
     1441 ccaaccgatt caaacaactg gcgggccgtc tcacgatcct cggacgacac ccccggcgca
     1501 agactcatcg ccgttacacc ctcgccgatc gcggatggag tattcggcat ggcgcggatg
     1561 agcctggtgg cggaaggaag atggtgttgc acgcgagaca agggataccc tgcagcaacg
     1621 gagatgatga ctgggtctga cggaagcgag ggcgcgagtt ctctcagcac atcatcgagc
     1681 acctgcggtt ccacgcagag caccacaatg tccgcgcctc ggaccgccgc ctgattgtca
     1741 ggcgtgacac taacgcccag gttacggctc aacagttcgc gtctggaagc aagcgggtcg
     1801 ctgacgacaa gacggccggg cgaggacagg cctttccgca acaggcccgc cagcaacgac
     1861 tcggccatgt tgccgccgcc gacaaagccg atctgccgtc cagtcaacat ggcgcgatta
     1921 tacggaggct gttcaagccg gcgcaacttg caaccgccgg aaggggttgt tatagagtga
     1981 ttgacaggac agccatcatc gtctttttga gcagaatgga gccgacatga gatgcatcct
     2041 ctcgcttgtc attctgggag tcctcctgct gttcggcgac ccgagctggg cccgccggga
     2101 ggccctgacc gccgagcaaa aaagccagat ggaacgaatc gaccgtgtgt tgatcacggt
     2161 cctcgctctt tccgacaagg gctctgtcga tgccggcccg ctcgccgagg ttgcggccaa
     2221 tcgcatgaag gaattcggct atacgccggt gaccgacccg acccagccgc acgatgtcga
     2281 gttgaagatc aaatgtgaac agaaaaagac ctgggagggc acgaccacga tgggaagcga
     2341 cgccgatctc cctgatgcgc catcccgtat ctggaaggga ccggcctgtc agctcggcta
     2401 tctgcttaat ggtaagaaaa tggcctggcg caaggaggtc cgcacagact tcgatgatgc
     2461 gcaggctgcg gcaacactgg cgaaggccgg cgatcccacg tcctttgcca tgggcaggtt
     2521 acaagcccgc ctcgaagagt acgattttcc tgccctcatc accgctgaat ggggacagga
     2581 agcacggctg tttcgtctgt acgacgatcc gaaaacgcca gtggcacgca aggtgaagtt
     2641 agtggcgctg ttcggcgacc tcttctccgt gaaagccatt ccgcgcttgc tggatggcct
     2701 caacgggccg gatcggagca tcgcgaaagc ctccgcactg gccctgggca atatcggcca
     2761 gaaagatacg atccccgtcc tcatcaagac gatgaaaaac ggtgcgcccg atttacgagc
     2821 ccccgcggca aaggcgttgg gaattgtcgg tgcgctgcat ggagacttca ccatcgtcga
     2881 ccctctgctt gaaaccctga acaccgaaga tgtcgccgtg aaaaccgagg tcgcctgggc
     2941 gctcggaaaa ttgccggata tgcgggccta cgaaccgctc ttcgcccttc agaaatcgct
     3001 gtaccacgtc catgaaaatg atgccgaccc gaatctcgtg aaattgaagg aagcggtcaa
     3061 ttggagcatc aagcaaatcg atacctggga acacgtacag tgaggaatgg catacacatc
     3121 ggccggtgcg agcagtgcct cgccgcatca atcctcagct tcttcatact ggtggccgcc
     3181 gccggaatcg gacaggctca agtgataggc gaagaggcgg agctcgaccg gctacgagcc
     3241 aaagcagaag acgcgatggg gaatgatgat gcggaagggg cggccatgaa catgggccgc
     3301 gccgcactaa tggcggcaca actcgggaaa cgtcagactg aaccggcgtt gcggcaagta
     3361 ttcaaatcca cagaacacct gtatcgatca caggagcatg gataccgggg gctggctctc
     3421 ttcaggcggg ccggtggaga acttcccgcc tctgccggcg tatgcggcag cctccaactg
     3481 gcacagcttg agctacaaca tgcccaggag accctcgcct catcggagac tccagaccga
     3541 cctggtgcgg tctcaccaaa actgcccgcc atccgtcaaa cagcagacga ttgggccatc
     3601 gtgctggcat cgatgatggg cgaatttcgc tgccccaact agcaggccgt tgaaaaagac
     3661 cgccagcttc gttctcgcat cgctctgagg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn naactagcag gccgttgaaa aagaccgcca gcttcgttct cgcatcgctc
     3841 tgaggctcaa cgtaccgcaa gggtacgcct cgcctcctcg cattgctgcg gcctcgctga
     3901 acgacctttt tgaacggcct gctctcaaca tcaattcacg aaaatccgac cgtgccgccg
     3961 gcgggctgtt tcgcatcctg ctctgctagt cgtcttccgg attgatgatg tgcaacccgg
     4021 caggacgaca ggcggccagc agttgtttgt ctgcgctgac gacggtcagc cggagcggct
     4081 tcaactccag ggctaaggcc aggtgcagaa gctgcggcga gcgaagggcc ggatagtcca
     4141 aaatcaattc cttcgcagcc agataggtgt ccatcgtcgg cgaaatgaac tggaagagtc
     4201 cttgcgaggc ttccatctca aacttgtata agaccgaata acaatcgtcc cgcgtgatct
     4261 ctccctgttg cgagcggacg gacaaggccg aataaaaatc cgtgacgctc cacatgggaa
     4321 ggatggcgac cttcccccgc ttcaccatga gcttgttgac gatacgcgtc ccccgctcca
     4381 tgctgtagcg cttgaccagc gccgtggaat caaaatagta atacggcatc ctacaccctc
     4441 cccttcaaga gactgtcggc caacggcgac tggagcttgg agaggcgatc ctgcagctca
     4501 tccaacggca attcctcata gtagccttgc ttgcgaaaga tgctcacaat atcgctcttc
     4561 agcacctgct gcgtatcggc ggtgagccgt tcccgcagat gttttttcat tgcctgactg
     4621 gacatccggc gtccgccgcc ggcctgttcg cccgccttcc gggtccctgt ctttgccatt
     4681 ccctctcctc tctctatggt gaattcgcgg ccgttaacga ctgagcggcg gctgccccgc
     4741 ctctttcgga caacctgacg gcgcagagga tgacaccgat aagtccctct ctccgtagat
     4801 atgcgcggcc accgcttggg ccaggtctgc tgaaaactcc tgctgcatga cgcgaaccgc
     4861 tttagccgct gcctcgcgtt ccgtcacatg cccttctttt ccgccttttg ataccgcacc
     4921 gatgacacga tgggtcgaga cttcttcaat tacggcatca gtacaccatc gcacgtagcg
     4981 gaactgggga tcagggactt ggatcggcca catccgcacg gttccggtga tcagcaattc
     5041 cgccgtagcc gctcctgggc cagagccgac gaccgagaac ccttcctgcg tcaggccctc
     5101 gatgagcgcc cgctcgagcg gttccgcctg ctcacctgac acctccacgg ccatccccat
     5161 attcgcagcg agataatgct ccaattcagc agtcaattcg gccacacgat aggcggcggg
     5221 atgcccctgc ccgttcgacc ggattgtgcg caggtcggtg ttgtaggctt cgcgaaggac
     5281 gagatttttg gcagctcgct tcagattgcg cacgtgtgag agcttgtcct gggcctgatg
     5341 cgcttccgtg acttccgtct gaatcgaccg atcgaggtcg gccagacgtt ccagcatcgc
     5401 cgattcggct tgcgcccggt tcatgcctgc cagagcatag tactgtcgcg ccttttggtc
     5461 aaaccaggta tcgaggactt ggacgttttc caacacctta tccgtggtca ctcgcgtgac
     5521 attgtccaga atcaacttgc gctgagtgct tgtctgtccg cgggattcga ccaccaggta
     5581 cgattcccaa tccttggcct gcgccgtaat ctccgcctta aagatgcgcg acaccgccgc
     5641 ataggcctgt tccgtcgcct gtgggcgcga atcggcctgg ccgacgccta cgagatattg
     5701 cgtggaagga aactgcgtac tgctgccgtc tatccaggat ggacgggatg gcccaccgac
     5761 ccagccgcac ccggcgcaca gcagcatgac cagagcgact aggctgattc gcgtgcctcg
     5821 atccgagacc atggcttcag cgtccctcac agcatcactc gttcgatcag aaacagcgtt
     5881 tgtcccgctc gaagaacgag agggcgcggc ccttctacct gcggcgttcc gttgcctcga
     5941 accatggctt gaatccggct ctcatattcg ccgggcggca cccacaggcg ggaaatttga
     6001 atctcatccg gcaaggtgcg ccaacttctg gtgtcggcgg attctgacgc cacggccaat
     6061 ccatgcgcga ggacgcccac cagtaatccg acccacggcg cgtcgccttt attcaccgca
     6121 tgctgcgatc ctcgggttgc ggcttcagcc gcggcaaatt tcacagctgc ccgggcgagg
     6181 gctttcgtcg taattccagg taagcggtcg gagagggacc gctcagccag ggcggtgccg
     6241 ttgtagacta actcggatgt cgcagaaatc ggctctcctc ctcgagggat caacgtcacc
     6301 gtctcgtgca ccacctgcgt tttctgcagg atcagtttgg gcagagccac ccgtacgact
     6361 cgcccattca agccatacaa cacgctgtcc gccacgcggc gctcctgacg attcgacgca
     6421 tggatgacac cgcgattcaa caagaccaac tgcagcgcgc tcagactgat agggatgtcg
     6481 agaaacgcgt cttccttgcg cggcgcgcgg ccattatagc cgatcacaac aacctgggcg
     6541 agattctgtt ggtcagcgcg aggcagccaa cgcgtctcgg gaaacaggcg ccgatattct
     6601 tcgaactcgg tcgtcatatg caacgcatcg gttgtcctca gtaaatctgc gcggagcatc
     6661 ggcggcatgg gcgtccgcgc ccagctcttc gtcgcatcgt agatgtcgta ggccttccgg
     6721 taggcgatga acgcgttatt cagatcgccg gtcgcctcgt acaacacgcc cgtcagataa
     6781 cgggcaaagg catcttcacg gtacccgtct ttttccttgg cggcatcgga caagacgttc
     6841 aggcgatgat cgatctgcct ggcctcgacg acggcttcca tcagttgccc catcgcagcg
     6901 taattcagcg ccttgatgat gttgatcatc acatgttcgt agggatcccc ttcatagggc
     6961 agcacgttat cgttggtcag gaaggctgcg gtttcggtcc tgatcgtcct cgtatacagc
     7021 cgttcgactt cctgtgccgc cgattcaagc tgggcattgc tctgcatgta ctcgccggcg
     7081 aggtgaaggg tcatcccccg gtccatactg tagaggagac gattgcgggg tccatattcc
     7141 gactccgcct ccgccatgat cgcatccgcc cgtcgagcgt cgtgtgccgc caggctttga
     7201 tcgatgagga gataatggtt ggatgacagg ccgcagccgg acagcaccca cagcaggcac
     7261 cacccgatga cagagtgcaa gggccaagaa atgaaacggc ctccctgggg ggaggccgtg
     7321 gtcgatggga ggtgaggtct caactgaata cgtctggcta aaaaatcgtg cgtttctttt
     7381 cgatgacttt tttgatcttt ttctggccga accaggactt gacgttgttc tccaggtcga
     7441 ccatctcgag atcgatctga tagaagaccg ctttcgctcc gtcctgttcg tcaagaatcg
     7501 tggcgatact tccccgcatc atataatctg cgccgatttc tttgcccggc gctttttgcg
     7561 tatcttcgcg ggcatgcaca gcctgttcgc ggcgttcttc ccgcacctca tcacgttcgc
     7621 ccttggcagc cacaaacgtg actttttgcg agttggtcaa ctcacgttcc aggtcattca
     7681 caaacgtctg cacgttgata tgctcatggc tcttattgag caccgtccct acaatcacaa
     7741 ccggctgacg attcttgccc ttggtaaagt tgcccagcca gggattggcg agggcttcct
     7801 tcaccatggc ctcagccacc atctgggagt ctgtatcgtt ccaacgcccg ctaaggtcag
     7861 tgacggtgcc cgtatcgacc cgcgtcacct tcgtctcatg gccgcacccg ctgactacgg
     7921 ccgcgcatcc taccaatagc cccaccgcac aggttcgcaa cactgaatgc tctcgccact
     7981 cgatcatgct gactcctccc ggccacagac ccggataacg cctcgactat ttcgtcgccc
     8041 gcttgtcttc ttctttttcc agacgatcga ataaccggtc ggcattctta cgcacgaagt
     8101 cacggacctg gctattgagt tctttggcct gctccagatt attcttcatg tcttcgaggc
     8161 tgagcttggt caggacgtaa tacgtgccgg acttttcgtc cttatatcgg tccatgggct
     8221 tgacgccgtt cagcgtgacc gcggaaaagg tcttcactgc ccgctcgatg ttctgctctt
     8281 cactgttgcg agaaaaatcg cccgccgtcg tggaggcagc ataatcacgc atcagatacg
     8341 cggtataggt ttcaaaggtc ttggcgattt ccgcccggct gcggttctcc gccgtgtccc
     8401 aggctaacgg ttcgttccgc acacccacca cggagccgac gccatagaag gatttatcgc
     8461 ttttttcatt gaaggcgccg gatccctttt tgacccagtt cgggggacca ccgcaggccg
     8521 tcagcccgat gaggagtacc agcatcaacg cgcttccggt gaacttcgag aaagccccct
     8581 ctactctgct catacgattc ctccttggtg tggcacctgt gagatccgat ggctcggttg
     8641 tatgcggcaa gatgatggaa gatcgacgtg tgtggttcac tgggtgacga gagacaatgg
     8701 cgcgaaaaag cgagttttta tgctaacatg cacccccaag actgtcaacg cgcaaccggt
     8761 tttgcggttg acgtctggac ctgcgacagg aaatattttt acgtagcata cgcgaaggag
     8821 atgaaacaga tggctgacgt tcagattgaa gacggcatca ttcgggtcgt tcaactcgat
     8881 attcaggatc cgaaggcagc cgcagtgctg gcggaatatc cccaagtacg ctggcccgag
     8941 atcacccgcc gggcattgaa gatcggactg gggtatctga agggcggagg caaggactag
     9001 tcgttgagtg agaatgaata ggtgtaggac ccgtcacggc gcaacacgtt taatcaagtc
     9061 tgacgatatg gtaggccggt caggactggt gctacggagc gggtttctca ccgggagcac
     9121 gatcaccttg aatggcgagg gtggcgcgct cgctctggcc gcctaattcc aaatatcggc
     9181 gaaaggcatc gaccgacttc tggggttcat tcaggtggtc ggcgtacaag gtgcccagtg
     9241 aaaaatagac atcggtatag gcgggattga cggccaatgc ctgcaacatc gcccgctcag
     9301 cttcctggtg ctggcctttc ttttcgaggg ccagcgccaa tgagtagtga gaatccggat
     9361 tctgcggcgc atgctggacc gcggcctgtg cagctgccag cgattcttcg aggttgggca
     9421 ggacctcaag gcggataaaa ctcttcaaca catacgcgtc gccgaaggac ggatcgtacg
     9481 tgatcgcgcg attgaggcgt tccaacgctt cttctgccga cttctgctgc tgcaataaag
     9541 caaatgcctg ttcatattga actcgggctt ccttcatccg gtctgcttcg gtactgggtt
     9601 ccttgccgac ggaaaacatg gccttccgcc cttcgaccag aatgacatca ccgtctccct
     9661 caagttgaag gagttgcgga tgttgggccc ccttggtctc acgctccacg tcctgcttca
     9721 cctgagccgc gagttcactg gccatgatcc agccgttttt attcaggtcc gccgcaccct
     9781 tcagcccggc cactagcgcc gtcacgaatg cgctctgccc cgacgtacga acaactgatt
     9841 cttctttctc ggctgccgtg agtacttgca ccgatcgttt ctcggtgtca tcttccggcg
     9901 acaatcgccc ttccagcgag agcggctgcg gcgcggtcac gtcccagccg cgcaggttgg
     9961 catcgaacac catgaggatg tgcttggacg ccgatcgccg gctgaactct ttgagttgat
    10021 ccagggtaat ggctttggcg ggattgttga gctgcgcgtc ccatggaacc acgaagcccg
    10081 aatccttggc ttgagaatcc tgcgtcaggc cggcatggcc ggtgaaaaag aagacgatgc
    10141 gatcctgccg acccactttg cgcggcaggt aatcgttgag gatatggagc agccgtttgg
    10201 aggccgcctc cttgtcctgg atttcgatga cctcatcaaa cccgagccct cgcaacgctt
    10261 cagcgactgc atgtgagctc tccagcgctc ccggcacctt gggcgcgacg agataattct
    10321 caatgccgac gacgacggcc catgatttgt aatagagtcc ctccggcttc ccgacctggg
    10381 cagccgcatg atcagtggac attccggcga cgaataaggc cgccaggcag gtgatcatcc
    10441 gggggaacgt gagagatctc ggcgaacgca tcagcaggat gatccagtga agggtaacgg
    10501 actcagccta ccctcgcgtc gtcccgactg tcaagaaagg ccatcggcgc caacgcttgt
    10561 cccacgcggt cattcgctgc gctccagcgg cacctctctg cggcttgagg gttgcagttc
    10621 ctccgcccca tccgcataat gccggagaga ttgaaccgcg catcggacag gcccgcgttt
    10681 tgtggaggta tggcgactca tgagtgagaa aatcgatacg ctattgaaag agggacgtgt
    10741 cattcagccg tccgcccgga taaaagcggc ggcgcacatc ccggattacg accaggcgta
    10801 caaggcttcc attgccgatc ccgaagcctt ctggggcggc gtggccaagg agctggaatg
    10861 gttctccccg tggagccggg tgctcgagtg gaactacccc tgggcgaaat ggttcgtcgg
    10921 cgcgacctgt aatattgcct acaactgcct cgatcgccat acgaacggct ggcgtcgcaa
    10981 taaggtcgct atcatctggg tcggcgagaa tggtgaagaa cgcatcttca cctatgggga
    11041 gctgctgcgc caggtcaacc gctgcgccaa tgccctgaaa gcccttggct tgaacaaggg
    11101 cgatcgcgtc acgatctatt taccgaaaat tcccgagcag gtcgtggcca tgctggcctg
    11161 cgcccgcctc ggcatcattc atagcgtcgt gtattccgga ttcagtgcgc cggccttggc
    11221 aagccgcatt caggatgcgg aagcgcgtct cgtcatcacc gccgacgtcg ggtacgaccg
    11281 cggaaaaacc attccgctca aacccgtggt cgacgcggcc gtgcaatcct gcccctccgt
    11341 cgaaaaagtc gtcgtggtgc gccgggatgc cctcagcgtt gcactcaccg cgccaaaaga
    11401 aatcgattgg gccgattggc tgaaagagca gaacgccgtc tgtgccgcag aaccacttga
    11461 tgccgaaacg ccgctttaca tcctctacac gtccgggacc accggcaagc ccaaaggtgt
    11521 ggtgcacgtc catggcggct acatggtcgg cacatacatc acgacgaaat acgtgttcga
    11581 tctgaaagaa gaggacgtgt atttctgcgt ggccgacccg ggctgggtga ccgggcacag
    11641 ctacatcgtc tatggcccgc tattgaacgg cgccacgatc ctcatggccg agggcaaacc
    11701 cgattacccc aaccccggcc gctggtggga cctcatttcg cgctatggcg tctccatttt
    11761 ttatacgacg cccaccgccg tccggctgtt gatgaaatat ggcgaagagt ggccgaagaa
    11821 gtacgacctg tccacgttgc gcatcctcgg cagcgtcggt gaacctatca atcccgaagc
    11881 ctgggaatgg ttctatcgcg tgaccggcag cgacaaaccc atcatggaca cctggtggca
    11941 aacggaaacc ggcgcgatcc tggtcacgcc cctcccgtct gtcccactca agcccggttc
    12001 ggcgacgcgc ccgttcctcg ggattgaagc cgacgtggtg gacaaggaag ggaacagcct
    12061 gcccgccaat gccggcggct ttgcggtgat caagaagccc tggcccgcga tgatgcgtac
    12121 aatttataaa gacccggatc gctacaaggt ctactggaac accatcccca attgttatac
    12181 ggcaggcgac gtctgccgga aagaccagga cggctacatg tggttcatgg gccgcgccga
    12241 cgacgtcatc aaagtggcgg gcaaccgcat cgggacggcg gaagtcgaaa gcgccttggt
    12301 gagtcacgaa gccgtggctg aagcggccgt catcggcaag ccgcaccgga ccgccggaga
    12361 agcgatcaaa gccttcgtca ttctgaagca gggctaccag gattcgaagg agttggtgca
    12421 gtccctcaag gaccatgtgc tgaaggaact gggaaaaatc gccgtgccgc aggaaatcga
    12481 tattgtgtca tccttgccca aaacgcgctc cggcaaaatc atgcggcgag tgctcaaagc
    12541 caaggagttg ggacaggacg tgggggatat ttccacgatt gaagactaag ctgatccact
    12601 gggggtcagc atcatctcta acgaaagcat ggaggccggt catgctaggg caacggacaa
    12661 cacgtggact cctgtggtca atggtcacag gcatcatctt actcggggga gtggttccca
    12721 ttctcgctgc ggacgataca cccaaagccg cgggccaggc tcccgctcag gcgaaaaaag
    12781 ctccggagaa gaagtcggtg aaaaagggag agggccggaa gaccaccacc gccccagtca
    12841 aatccccggc gaccaagtat gcctcggcag aaacggcagc caaagctcaa gcctgtttcg
    12901 gggaagcgcc caaaatcgaa tccgtcaaac ccgatgaagg aaaagccggg gagaaggtca
    12961 ccatctccgg gatgaatttc ggcccggcag aatgtctgcg atccctgtcc ttcgggcctg
    13021 gaaatgccgc gaccttcaag ctcgacagtc cgactcaaat caccgccacc gtccccgcga
    13081 gcaagcgcaa aggcatggcc atcatgaccg tcaccacagc gtccggcgaa gactccaaag
    13141 ggtttctgct gaagtagctg atctgtccta gccaattccg gcaacacggg atgatcaaaa
    13201 ggccgtccag gcagaccagt accgaagccc acggcgcgag aagcctgaag ctcaacatgt
    13261 gtgggtacta gggaacgatg agccgggaag agtcttaggc ccgaggcctt cccgggtacg
    13321 tcgaggatct gaacgaagcg agaacgccgc tggcgaactt tttcagcatc ctgttaaatc
    13381 aggccgcggc gctgcaaata gtccttgtcg atgacggggg tgggtttcgg cggaagaagc
    13441 cctcgatcct gcagaagccg ttcgacgtac ttaccgatca ggtcggactc aagattgacc
    13501 ttgtcgccta cctgtttcag accgagcgtc gtcactttgg ccgtgtgagg aatgatcgcc
    13561 acgaggaatg agcgctccgt cacctcgttg atggtcaggc tgataccctc caccgtgatc
    13621 gaacctttgt gaacgcagag acgcaagatg tctttgggcg cttctatctc caggaccagc
    13681 gcattgccat cctgatgccg gctgcgaatc gcgccgatgc cgtctacgtg gccggagacc
    13741 atgtgcccac cgattcgttc gttcagtttc atggcgcgct ctaaattgac aggcgatccg
    13801 gtggtgagat cactgagggt ggtgaccgag agggtttcgg gcgacacgtc caccgagaaa
    13861 tcatgatcgg ttctggcgac ggctgtcagg catgcgccgt tcacactcac gctggcgcca
    13921 atcgtcagat cggtcatgat agtcgaggcc atgatggtga gccgggtgcc ggccaacccc
    13981 ttattcagga tggaaaccgc gcccatctcc tccacaatgc cgctgaacat ggcttacgcc
    14041 cccttgtgcg tcttcaagag atagagacaa aatccgagca atccaacccc gcccacgatg
    14101 gcaatcccgc gttgaagcgt ttcgataaaa tccgtatcca tcctttgctg cgtccttgtg
    14161 aaaaaaagat tgtctagtgc tgccctgaca aatgcggcgg cattatacac taccgcaaaa
    14221 tggttctcat cgtcgcccag aaggcgacgg ccgcaagaat ttggatgccg gcgatcacgg
    14281 caaaggctcc ctgatagctg gcgtgggcga tcaaaaaccc ggccaggagc gggccactgg
    14341 catgcccgat atccatgacc gttccctgca ttcccattcc cgcccccaac cgtttgaatt
    14401 ccgaactgtc ggccaccagg gcggccgatg aggatgacac caccgcttcg ccgaacccga
    14461 acccggagga cagcatcagc agcacagcga atgatccgac gtggggaatg ctgatgaacg
    14521 tcgccgcgca aatgaccagg ccgagcacga tcaacggctg ccgaccaacc cgatccgata
    14581 tgcgccccat aaccggcttg gacaaaaagg acgtcaccgc ctgcacactg aacatcaggc
    14641 cgacttcacc ggcgttcagg ccgactgaca ggccgtagag gggcagaaac gccatgagcg
    14701 cgccattggc gatcatcttg gcggcatcgg tggaactggt aatcaacact ttccggttcc
    14761 gggccactgc gagaaaccct tttcccatct cggtgatcac cggagccagc cccttctcgc
    14821 gtacacgcgg aggcggctcg tccaaatgga ggctgaagaa gatgagaatg gcgatgcacc
    14881 cgaagacacc ggccgtcaca aatgccgctg gaaaccccgc cgcatgggcc aaccatcctc
    14941 caataaaagg gccaaggagg gaaccggact gggtacaggc cgtataggtt cccatcgccg
    15001 cacctcgccg ctccttgtag agatcagcta ccgtggccag ggcgctgggc gcgaagatgg
    15061 ccgtcgccag gccgtggaca aatcgcaacg ctgtcagcgc gttgagatcg gaaatgaacg
    15121 gatagataaa gggcggcaac ccgaacgcca ccaccccgat gcggagcagc atcttccgtc
    15181 cataaatatc ggacagggca cccgacggca atttcagcag caccccggta atcgtcgata
    15241 ccgagacgat caatccaatc cgctccggcc cggcacccag cgattccgcg aagagggaca
    15301 ggaccggcat gcgaacgagg ttatagctaa tgaaacaaaa tacgccgagg gtacagaggt
    15361 agaaaaagct gcgggacgtc gtcatcactt tctcatggaa gggcggggct cggcttctgc
    15421 ccaggattgt ggagggacgc gagggcggct ttgagaaacg cgacctgttc atccggaaga
    15481 ccggggggat ccgtcgcctc gaagagcacc tgatccggat ggtcgcgcat gacgcccgcc
    15541 aaacgacggg cggcatcaaa cgctttcacg acctgacgag tgacgacatt gccgatgagc
    15601 ggacgcagca tcgagagcaa cccggagtac aaccggttat cgagccgcag gtaggccacc
    15661 atcgtattgt ccatcgattc gctgccctgc ccgtctttca ccggctgcaa tttgaacaac
    15721 accagagcct tgccgctgac gcggggcagg aatcgtccat catgcgtccc ttcaacgtag
    15781 taaatccgcg tgctccgatc ctgatacaac ggatgcacga tgccttccgt gccttcaccg
    15841 tccgtcgccc agaattcatc gggaccgcgc cactcagctt tgtacaaccc gagatcgaga
    15901 cgattcacga gggccgcggc catgaccgga tgctccaaca gatagagata gacatcctcc
    15961 ggcagcaagg tacggatcgg tcccacctta ttggccgtgg tgtaatgcgc aatgatcggt
    16021 tcaagccggc aggtccaggc agcatctacc tgatcgagag ggaacaccat gccgacatgg
    16081 tgcgacggca gggaacagcg ttctggttcg gcctgcccgg tggaatcgca gagagccaag
    16141 gcggcggtga gcacagcggc caacgggccg attcgccgaa ccatctcgcc gcccccggtc
    16201 atgaagcccg ttgaggcacg gtcacagtca cgtgagcggg aaaaagctgc gcaggatcct
    16261 gccggaggcg agcctgccgt aacagcgtct cgacatgtgg ttcgagaagg ccttcgaatg
    16321 acggttgccc gtctgtggta atgacgaccg gtttggacaa atcgaccagg tcctcattca
    16381 aatacaacgt gtactgccgc acgcggtcgg tccgcacgtc gacccgattc ggaccggtca
    16441 cctgtgcggt caatcgggca tagatccgct tccgaatgct gtcgtcgcgc ttgtcgacca
    16501 gatcttccga aaaggatgcg atctgatccg ttgcatcgat ccgcacccac ccgaacggca
    16561 ggagatgact cgcatcccgc accacggtca cggtcttggg aaccggcgtg cggtgctggg
    16621 tatcgaacca cttcaccaga tcaggcagct cttcacgcgg gaagaaatgg ccgccggcca
    16681 tggcatgggt ccggtcatgt tcacgataga tgtaggggta atcgaggttc ttcagctcac
    16741 ccgccagctt gcgactcagt tccacgggca tcacctggtc ttgagagcca tgaatgatgt
    16801 agaccggcgt ggtgcggaga ttttccagga acggaaataa gacatcgtcg atgccactgg
    16861 ccatcggggc aagccctgca aacaggggcg catgatgcat accgatcagc cacgcgccga
    16921 taccgccgtt ggacatccca ctgagaaaca tccgattcgg atcgattcga taccgctccc
    16981 ggacgagttg caccgtcgcc agcacgagat cttcggcccg cctggtaaac caggcacccg
    17041 cgggataggt tggacaggcc aggatatatt gttcgcctaa gcgcgacttc catcggtcca
    17101 aataggcttc accggtgaac cccgcgccat ggagacatac caccagcgca tagtctttag
    17161 tcggctcgta actctgggga atggacagac tcacccgata gggccggtcg cgaaccatga
    17221 cctgttcatc cgccaacacc ccgacaggct gaaggccatg gggctggccc ttgcgcagga
    17281 tgtcctgcac ggcctgaagc gttgcatcag gccgctgcat gatggacgcc agcgccgaat
    17341 tggcctgctc gctgtcctga gtcgtgaggt attgccagac ctgcgccgca agatccgacg
    17401 aggatgctgt ttcggctgca tgagtcaaag aaaccgacaa cagcgccaac cagatgcaaa
    17461 cgagggacag gagcagaccg gggcgccgcg gatgaaccct gtacgcaggg cggagagaag
    17521 atcgcacaat actcctatcg agtcgggaag tcagcctgga tcagcatatc acgcccgaca
    17581 ggcgtcatgc gtagattgtt gaggagcacg gcttcccgca ggcggcgcgg tgacagccct
    17641 cccaacaccc ctttcgcatc ctgcccgccc agcagcagcg gggcgacaaa acacatcaag
    17701 cgctggggca gtcccgctcg cagcacggcc gcattgagat cgctgccgcc ttcgacgaga
    17761 acactcgtga cgccgagctg tccgagcctc gcccataatg ccgtgagatt gacgtgtccg
    17821 ccggcagcgg gcaacaccag gacatcgatg ccctggcctc gcaagcgctc gacctttgtt
    17881 actgaggccg cgcgcgtggt ggcaatcacg gtgtgggctt cttcctgccg tttcagcacg
    17941 gcggcccggg acgggatacg tagtcggctg tcgaccacca cacggagcgg ttgcctggcg
    18001 gcaaggcgga cgggatcacc acccgtcctg gcggtcaact gtggattgtc gcgcagaacc
    18061 gtaccgatcc cgacaagcac ggcatcgacg tcggcgcgca gctgatgggc ctgcatccgg
    18121 gccgcttcat ccgtaatcca ttgagattcg ccggtggcgg tggcgatctg accatccaac
    18181 gtcatgccgg ctttcagaat ggtgaatggc cggccggtct gcacccaatg gatatacgtt
    18241 tcgttgagct gtcgcgcctc aggttcgagg catccgactt cgacctgaat gcctgcccgc
    18301 ttgagctggg cgattcctct cccctgcacc tgtggattcg ggtccaccat ggcaacgacg
    18361 acgcgccgaa cgccggagga aatgaccagc ggtacacaag ggggcgtccg ttttttgaga
    18421 tgactgcagg gttcgagggt gacgtagaga gtgccgcctt tggcgcgtga accggcctga
    18481 cttaaggcga gcacttcggc atgcggacca ccggccttgc ggtgagagcc ctgtccgaca
    18541 atctctccgc ggttcacgac caccgcgccg accatgggat tgggaccggt atgaccccgg
    18601 cccttcgcag cgaggcgaag ggccagggcc atcacttcac gatcgcggga ggataatttc
    18661 accggctcgt acgcttggag cggcgcacca cgcgtttcgc aaccggccgt tttttcgccg
    18721 gagcagattt cgccgacttc ttgacagact tgacggcgct cggcttcgcg gccttcggct
    18781 tgctcttcgc cggcttgggc ttctctttct cggcttccgc caacgctgct tgcgccgcag
    18841 ccaatcgcgc aattcccact cgatacggag aacagctcac atagtccaat cccaattggt
    18901 gacagaactc cacggaactg ggatcgcccc catgttcgcc gcagatgccc aacttgatgg
    18961 ttggacgggt tttacgtcca ccggtgatcg ccgtgcgcat gagggaaccg acaccttcac
    19021 ggtccaacac cgcaaatggg tcgctctcca gaatattggc ggttttgtag aaatcgatga
    19081 actttgccgc atcatcgcgc gagaagccga aagtggtctg agtcagatcg ttcgtgccga
    19141 acgagaaaaa ctcggcttct tccgcgatac catcggccgt cacggccgcg cggggcaact
    19201 cgatcatagt gccgaccaag tacgacaact tgacgccata ccgcttcatc gtctcggccg
    19261 caacttcccg gacaaggtcc ttctgggcct tcatttccga gaccatgcca accagcggga
    19321 tcatgatctc cgggacgatc ttggtgcctt ctttcgccag ttcacacgcc gcttcgatga
    19381 tggcgcgcgc ctgcatcttg gtaatttccg gcatcgtaat accgagacgg cagccacgca
    19441 ggccgagcat cggattgaat tcatgcagtt cttcgacgcg agtcaacaac cgcttcttct
    19501 cctccagcac cgtctgcgac ccactggtga gttcgagctg agcgatttcg accatgaggt
    19561 cctcacgctt cggcagaaac tcgtgcagcg gcggatcgag caatcgaatc gtgacgggga
    19621 accccttcat ctcacgatac agaccgataa agtcctgctt ctgcaacggc agcagctgat
    19681 ccagatacat ttcccgctct tcgcgcttcc gggcgagaat cattttttgc atgatctgga
    19741 tgcggtcctc ggcgaagaac atatgttcgg tgcggcagag accgattcct tcggcgccga
    19801 aactccgcgc gatacgagcc tgatccggca catcggcatt cgcccggacc ttgagcttgc
    19861 gtaccccgtc ggcccacttc agcacggatt cgaacaattg atatttctca gaagccgtgg
    19921 cctccatttt cccctgcaag acctgaatga cttcggattc caccacggga atatccccgc
    19981 cgtacacatt gccggtcgag ccgttgacgg agaggtagtc gccctcacgg aaggtctgcg
    20041 tcccgatccg taccgtttga ttgtccacca cctcgatggc ttcacaaccc gccacacaaa
    20101 ccttacccat ctgcctggcc acgacggccg catgggaggt catgccgccg cgcgcggtga
    20161 gaaaacccag ggcggcattc atgccatgga tatcatccgg gctggtttcc tgccggacca
    20221 acacgacgcg gttacctgcg gcttgcatct ctaccgcccg atccgccgtc aaagcgattt
    20281 ttccggcggc ggctcccggg ccagcgggca atcccttgcc gagcggcgtg cagtgcgatt
    20341 cttccttcgc atcgaaaatc ggatagagat actgtgccaa ctggtccggg ccgatccgct
    20401 gcaaggcttc cttcttcgaa atcagtcctt ccttgaccat atcgaccgca atccgcaccg
    20461 cggccacgcc ggtccgtttc ccgacgcggg tctgcaacat gtacaacttg ccttcctgga
    20521 tggtgaactc caggtcgagc atgtcgcggt agtgccgttc cagtttctta taagtgactt
    20581 ccaaatcttt gtaggcctgc ggcatgaact tttcgagctg attgaccggc aacggagtcc
    20641 gaatgccggc gacgacatcc tctccctgcg cattcgtcaa gcattcaccg aagaattgcc
    20701 gctgaccggt ggccggatcg cgcgtgaagg ccacgccggt cccgctggtc tcgcccatgt
    20761 tgccgaacac catggcgacc acgttcaccg ccgtgcccca ggtttcggga atattgtaga
    20821 ggcgacgata ggtcacggcc cgggcgccgt accaggagga gaacaccgca ttgatagcca
    20881 tgcgtagttg ttcgagcgga tcatccggaa aatcgcgctt ggtttcctct ttgacgagtt
    20941 ccttgaaact gacgacgagt tccttgagcg ccttcgcatc gagatgcgta tcctgcgtga
    21001 ccccgagatc gcgcttttta tgtttgagaa tgtcctcgaa atgctcgcga ttgatgccca
    21061 tgacgatgct gccgaacatg ccgatgaagc ggcgataact atcctgcgca aaccgttcgt
    21121 tcttggtctt gagcgccaag ccgtgcacgg tcttggtggt caatcccacg ttcagcacgg
    21181 tatccatcat gccgggcatg gaagcacgcg caccggagcg aacggagacc aataacgggc
    21241 tgtcgggatc gccgaagccc gccttcatcg accgctcgat cttcttgagc gccaccaggg
    21301 cttcctccat catgccggga gggtaggctt tgccccgctt gtaatattcg acgcaggcct
    21361 cggtggagat cgtaaacccg gggggcacgg acactttgag attggtcatc tcggccagac
    21421 ctgcgccttt gccgccgagc agttccttca tgttgccggt gccttcggct tttccatctc
    21481 caaagtaata gacgtatttc ttggccacgc gacgctctcc ttctgctatg aggtcaatga
    21541 gtcggaaccg aatggcgggc ggcggcttcc agtcgcatac gatagcggtt gacgacgtgc
    21601 ctggtcacgc gaatgccaca cacgatgatc cccacgacga acgcgccgac cgcaaacaga
    21661 cgatagtcca cgaacggtcc gtagtcgaaa tagaatgatc cgtctgcacc ctgcttgagt
    21721 tgtgcgtctt tctgaaacgt atgggttttt ccggttggat cggcgaggtt gagccactcc
    21781 gaacagaact gcacgggatt ttccgagccc ggaacatgtt ggtaggccag tcgcaagcaa
    21841 atgtcttgtc tggaatcata gatatccggt gtccgcacca gccgttcaaa attcaggccg
    21901 gtcacccaga tgccgaacag gaagaccgca ttgcacacca ccatcatcac gacgctgatg
    21961 ccggacgcaa atcgccggac tttggtcgct cttacttgag ccgaggcact ggcctcgggt
    22021 gactcagggt ggctctctga agttgagtcc aaatgctcac catccgctac gtcccttgta
    22081 ccataatctg ggagaaatca gcgaatgaca tgaacaaatc atcgacctct ttcaagagtg
    22141 acagccggtt tccacgcacg gccggatcgt cggcgttgac catcaccgca ttgaaaaagt
    22201 cgtcgatcgg acccttcatc cgaaccagca cgtccagcgc ctgtccgtat tgcccgccgc
    22261 gcatggcggc cgcataggca gcatggcttt gctgcacagt ctcatataag gcggactcgg
    22321 caggctcccg aaataacccg gactccaccg gcttacgatc ccactgttcc ttttcggtca
    22381 gccgatgcgc gcgtttgaat ccaacgatca gcggatcgaa ctccgccctc gacgtaatct
    22441 gctgcagcga ctgcatcttg tcgaagaggt cggtgagatc gatggactgg cggtcggccg
    22501 atttcagaac agcctccatc acatcgtccc gcaattggtg cgcgcttttt ccgtaaaaac
    22561 ggacccgctc aaaaagaaat ccgatcacgt ccggcgcgcc gcctttggcc ggctgaggcg
    22621 cagccgccac atgctgaccg gccaaccctt cctgggtcgc ccgcactgct tgcgcgagat
    22681 tgatctgcaa tcggctctcg atgagaatac gcacaatcgc cgtggcatgg cgtctgaggg
    22741 cgaagggatc ttcggagccg ctcggaacca gcccgaccag gaaaaatccg gcaatgctgt
    22801 ctaaccggtc ggccagagag agcactttcc ctgccagcga ttccggcaac tctccttcca
    22861 tcgcccgtgg cagatactgt tctcgaatcg cggcgctgac cgtcgccgat tccccgtcgt
    22921 gtttcgcgta ctccccgccc atcatgcctt gaagagtcgg gaactcgccg acgatacccg
    22981 tcaacaaatc agctttgctg agttccgccg cgcgccggca gtcgtgaacc aattggtcgt
    23041 caccaagctg cccggcgaga tgagccgcca tcgccacgac gcgctgcgtt ttctgatgga
    23101 ggctgccaag tttctggtgg aaggtgaccg cgagctgctt tgccacacga tccgccagcg
    23161 acgttttgcg atcctcgtcg aagaaaaatt tcgcgtcggc caagcgcgca gccagaaccc
    23221 gttcgttccc ctcacggatc aactgcatgt tcgccaactt catatttgtg accgcgagga
    23281 aattgggcaa cagcgcgccc ttggagtcca cgagggaaaa atatccctga tgctccttca
    23341 tcgaggtcat caggatttct ttcgggagcg ccagataatg cggtttgaag gaaccgagaa
    23401 tcgtcagcgg gcattccacc atgtagacag cctgctccag caactcctcg tcctgatgca
    23461 ggtgcccgcg tgctgatttg gccaacgcgg ctagttgatc gaggatcatg gcgcgccggc
    23521 gatcctgatc gacgatcacg ctgtggcgtt cggtctcttt gaaatagtgc gcgatggatt
    23581 gcacggcgaa acctttgggg ctggacacct tcgcacccag cactcgatgc ccctggctgt
    23641 aattgccggc cttgatcgtc gcgaactgaa tcggcaggac cttgcccccg cagagcgcaa
    23701 tcaaccaacg caccggccgg gcaaagcgca ccccggtctg gttccaatgc atggccttgg
    23761 gaaacgacaa cttggccaac aactgcggca acgcctgcgt caggaccgca gccacggcct
    23821 ggcccttctc cggcttcacc gcaaacagat agtcgccttt gggcgtctgc cggacttgca
    23881 gagactcaac cggcacgcct tgacccgcag caaacccaat ggccgctctc gtcggctgtc
    23941 ccgactgatc gaaggccacg gcttttgaag gccccatggc ttctttgacg gcggaggcct
    24001 gctgtcgcgc cagcccttcg accaacaaca cgagccgccg cggtgtgccc atcgtccgga
    24061 cagtgccgta ggtgagacgt gagtctttca gcaacgtctc cgctccctgc tgcaaggcgc
    24121 gtaacgcggg agcgacaaac tgatagggca actcttccgt cccgatttcg agcagcaatt
    24181 ccgtcgtaga gggagtcttg gcgcgtgagg cagatgcagg cttcttcgcg cgtgaaacgg
    24241 tcttcgtctt gggtttttta gatggcattg tggaaatagg atccgcgggc tacttggtag
    24301 tgaccggtga gtggttgatg gttcccttac ctgccccagc cggttgcttg atcaaaggat
    24361 ggcccatggc ctcccggtcg gccacgtacg attccgcgca gcgacgcgcc agcgcccgca
    24421 ccctggcgat gtaggacgtc cgctcagtga cgctgatcgc cccgcgcgca tccagcaggt
    24481 taaacatgtg cgaggtcttg atgcagtaat cgtaggctgg cagcgtcaac ttcttgtcca
    24541 gcagtcgctg acattctcct tcgaaggcct ggaaggtggc catcaacatg ggcacctcgc
    24601 cctcttcgaa gttgtagcgt gaaaactcga cttcgctgcg gtgatgcacg tcgccgtagg
    24661 tgacgccgtc actccactgc agatcgtaga cgttatcgac ctgctgcaga tacatggcga
    24721 tgcgctcggt gccatacgtg atttcgccgg tgatcggatt gagcggaatg ccgccgattt
    24781 cttgaaaata cgtaaactgg gtaatctcca tgccgtcgag acgcacttcc cagcccaacc
    24841 cccaggcccc cagcgttggt gattcccaat catcctgcac aaaccggatg tcgtgctttt
    24901 tgggatcgat gcccagttgc ttgaggctct ccagatagag tccctggata ttgtccggag
    24961 ctggtttcaa aactacctgg tactggtagt aatgctgcat gcggttggga ttttcaccgt
    25021 agcggccatc ggtcggccgg cggcaggctt gcggatacgc agcggaccag ggttcaggcc
    25081 cgagggagcg gaggaacgtg gcgggatgaa atgttcccgc gcccacttcc agatcatacg
    25141 gttgatggat gacacaccca cggtcggccc agaagcgatg cagagtcaaa atgaggtctt
    25201 ggaaattcac cggatgtcca gatctagccg caggctgctc gtggtgacgg acaaaaatca
    25261 cgctgaagct agcaagaacg gttacagggt gtcaagaaac ggagcgtccg ttttcaatag
    25321 gttgcaggat ggaaccggct acgcagtgga acggatttct tcttcgccac ctcgtggtcg
    25381 aatcgcttga atcgcgagac gcgctggttt attctgccaa atcttgagca actcactaga
    25441 caactacctc gaccatgaag ttctccaaaa agagtgaata cgggctccgg gcgcttctcg
    25501 agctctgcga gacgtatgga gggcgcgtcc tccagcgcca tgagattgcc gagcggcaga
    25561 acatccctgt ggaatttctc gagcagattc tgctcgccct caaacgcgcc gggctgctgg
    25621 cgagtcggcg cggcattcgc ggcgggtatt cactgatcaa atcccctgaa gaaatcacgc
    25681 tcgggcaggt catccggatt ctggacgggc cactggcccc gattagctgt gtgagcaaaa
    25741 ccgcctatca aaaatgctcc gactgtcctt acgccgctaa accctcctgt ccgctccagc
    25801 aggccatggg ggaggtgcgc gatgccattg ccaacattct cgaccattac accttgagtc
    25861 gatttgccca taccaaactg gcggaaggat cctgatgcgt accatcaccc atacattttc
    25921 gaccctgatg ctcagcgtca tcgcgtcctg gggtctgttc acgttcccgg ttgcaccgag
    25981 ccacgcagcc gagacgcgcg atttgattct ggcggcctac agcgtgccca aggaagccta
    26041 tgaacgacac atcattcccg ccttccaacg gtattggaaa cagaagacgg gacaggatgt
    26101 gcgggtccgc agttcgtacg gggcgtccgg agcccaggcg cgcgccatca tcggagggtt
    26161 cgatgcggat gtcgccgtgc tctcgctgga aggcgatgtc gatcaaatca ccaaagccgg
    26221 cctgattacg catgactgga ggaaagctcc gcatggaggc atgatctccg cctcggtcgt
    26281 ggcgctcggt gtccggaaag gcaatcccaa aggcgtgaag ggctgggaag atgcggcacg
    26341 gcccggcgtt gaagtgctgt atcccaatcc caaaacctcc ggcggtgcca tgtgggatgt
    26401 gatcgccatt tatggcgcag ggctgaaact cgccgaacaa cgggcgggaa aaccggtggc
    26461 tccggaagcg gcggcgacgc aggcggtcga cttgctcacg cgcattcagc gcaacgtgaa
    26521 ggtcatggac aaaagcggcc gggaatcggt gacgacgttc gaacgcggcg tcggcgatgt
    26581 gatcgtgacc tatgaaaatg aattgttgcc gcgcgtgaaa agcgggcgcc cctacgaact
    26641 cattgtgccg gccgaaacgg tgtggatcga aaacccggcg gcggtggtcg atacctatgc
    26701 cgaccggcac aaggccaagg atctggctga agcgttcgtc gctttcctcc atggtccgga
    26761 ggcgcaggct gcgttcatcg aactgggatt ccgtccgttg gaccgtggcg cagtggcgcc
    26821 tgcttcgggt gccgccctgc ctcaaccggc ccggctgttc accattgcag accttggtgg
    26881 atgggagtcc gtgagcacca aactcttcgg cgcacagggc gtgtggacga agattgtcga
    26941 agacgtgtca aagaaatagc aggaggctga acaggccagc cagcggcgtt ctcgcatcgt
    27001 tcaggccctc aacgtaccgc ccatgggaca tgagtctgtc tcgccaggct cagggtgggc
    27061 gggtaagaac atgcagctca ggccttcact cgctgcggcc ttgctggact gacctgttga
    27121 gcctcctgtg aatgaaccag taacacatga tcgccaccac aagtggaaat agttgcatgc
    27181 caaacataaa tgaccgtcac cacagacacg gaacgggcgc atgacctcac acatgctctt
    27241 gcgtgtggca ctacggtctg cagcggtggg gtatgtcctg ctgctgatct ttcttccgct
    27301 ggctaccctg acgcagcaat ccatcgcctc gggatgggac cgtttcattc aggatttatc
    27361 ggcccctcaa gcggcagccg ccctctggct gaccattgaa acatccgcga tcgccaccgc
    27421 catcaatgcc gtcttcggca ctctttcggc actcgtgctg gtgcgttatg aatttcccgg
    27481 ccgctggctc ttgaatgcgt tggtcgatct gcccttcgcc attcccactc tcgtcgccgg
    27541 gctgatgatt gccgccattt atgggcccac gagcgtgctc ggcacctggc tccagcaagg
    27601 tggcctgacg gtgctgtata accagcccgg catcattctg gccatgctgt ttgtcaccat
    27661 gccgtttacg attcgctccc ttcagccggt gttgatggga ctggaacgcg atcaggaaga
    27721 agcggcattc accctcggag cgagcccctg gacgaccttt tggaaggtga cggtcccgtc
    27781 ggtccttccc gggctgctga ccggagtgtt tctgaccttc gtacgggctt tgggcgaatt
    27841 cggctccatc gtcatcgtgg cgggcaatat tccgatgaag actcaggtgg cgtcggtcta
    27901 cgtctacgga gaaatcgaaa gctacaaccc ccaggccgcg acctccgtct cggtgctgat
    27961 tctgctcatt tcgtttgtgg tgctgctgct cttggaacgg ttgacccggc gtcccggtga
    28021 acggttgccc aaactgtttc gctgggcgaa ccggcacgat cagcccggcc gtgatgcggc
    28081 agcggcgacg gcggcgcact gaaaagccag ccgggtttga gattgtgtga cgtgtaatgg
    28141 gagtgcgggc atgcgttggt tgttgatcgg aatcgtctgg ctctattttc tggtgctcct
    28201 ggtcggcccg attctgtaca tggcgagcca aagtttcagc gaggggctgg cggcgttctg
    28261 gacggagatc tcccggccgg aagcggtgca tggcttcacc ctcaccgcgg aaatcacgct
    28321 gatcgtcctc gtgctcaacc tgattttcgg aaccctcacg gcgctcgtgt tggcgcgcca
    28381 acagttctgg ggccgcagcc tggtcagcgg cgtgatcgat ttgccttttg ccgtctcgcc
    28441 cgtgatcgcg ggattcatgc tgatcctgct gttcggaccg gacaccatgc tgggcgcgtt
    28501 gttcggccag gcgcatatca agatactctt cgcgctgccc gccatgattc tcgccaccct
    28561 gttcgtcacc tttcccttca tggtgcgaga actcacgcct ctgctgcaga cgctggggac
    28621 ggaagaagaa gaggccgcac ggacattggg ggcgggcgag tggcaagtct ttctcaaggt
    28681 gacgctcccc gccttgcgct ggggtctggt ttatggcgcc acgctcaccg tggcgcgcgc
    28741 catcggcgaa ttcggagccg tgctggtcgt atccggcaat atcctgttgc tgacccagac
    28801 tgccaccttg catatctatc aaagttatgt cgatttcaat tacgtggcgg ccaatgccgt
    28861 ggccttgacc ctgctcgccg tctccttcgc gatcctcacg gtgctggaga tcgccaaggc
    28921 cagagcggaa acgaccgtcg cagaagcagg agcgcagtaa gccggtgaaa atagaagtcc
    28981 gcgggctcac caaaaaattc ggttccggaa cggcggtcgg caatgtttcg ttcgtggtgc
    29041 acgaaggtga actgctggga ctgctcggcc cgagcggcag cggcaagacc acggtgctgc
    29101 ggttgattgc aggcctcgaa gtcccaacct ccggcgacat cttcatcgat gggaagcgcg
    29161 tcaacgacct caccgtgcag gaacgcaaca tcggcttcgt gttccaacat tacgccctct
    29221 tcaagcacct gacggtcttc gacaacatcg ccttcggcct caagatcaag aagtggaacc
    29281 ggcaggatat cgaacagcgt gtactggagc tcctctcatt gatgagcctc gacggactcg
    29341 gtagccgcta cccgcaccag ctttccggcg ggcaacgaca gcgcgtcgcg atcgcccggg
    29401 cgctggcacc ccgcccctcg gttctcttaa tggatgaacc gttcggcgcg gtggatgcga
    29461 aggtccggca ggaattgcgc gagtggctca tccgcctgca tacggatctg aacgtcacca
    29521 gcctcttcgt cactcacgat caggaagaag cgatggaagt atccggccgc atcatcgtct
    29581 tctccaaagg caagctggag caggccggat cgcccgctga tgtctacgaa gaaccagcca
    29641 cggaattcgt cgcgcgtttc atcgggtcga tgaatatcgt ggaaggcatc gtgcggcgca
    29701 atcaggtgca ggtgggctca ctcgaattcc ccgcgccggg attttccgac ggccagaagt
    29761 tgcaggtcgg cttccgcccc tactacgtga aggtggccga agatgcgacc cgctaccgtc
    29821 agcaggccaa actccgccac atttattttc tcggtgtcgc gtatcggttg gaaattgaaa
    29881 cggcggacgg attgattctc cgctcacgca tgaacaaaga agagtttcgc cgctgcaatt
    29941 ttacggtggg gcaagccgtt tcgtttgccg tcacccactt ccgcatcctg ccgcaagaag
    30001 gcgctgaggc gtcgagcgag gccaagcccg gcagccccat cacgggaccg ctgacgccat
    30061 aacccgaacc gagccagcta gtggagcaaa ctagaggatt ccacccccac cttgatcttg
    30121 gttattcagt agaaggcgtc tcagggaact gcccactggc aaaggcgtaa tccccttcag
    30181 tcgtgctgac aaaccacaca atttcacata ttgtcggatc gcgacccaac tcgatccact
    30241 cttgctcaat tcggccgata atcttgtcaa cagacagatt ccattgccta aattttccta
    30301 agccaaagtc cacatcaccc gccatgatat agcctgcctc tattaagtca cgtatgatct
    30361 caagggcatg agcttgaatt ttgtcggcgt cagtgcttga gtgaaggggc ttgatccgtt
    30421 ttactaggag ccaaagaccg gtccaatcgt caacgcaata tgcaagaaag tcgccgtgca
    30481 gaggagtcaa tttacgcatt actttatctt cactcaaaat ggaaccggtc aagagcttca
    30541 aaaaactttc ggactcaaat ctattgctga gctatcacat gcggaattag attacatccg
    30601 atcgtccttc cactgaaatg cagcgatacc ttttcccgca atcttctctg atgctgtgac
    30661 gagaaattct ttatcctgac ttccggagat acagcagccc gctcccaggg aaaggcacgg
    30721 taagcatgcg ctcgctgccg ccgatgaggg aatctgcttg tttagtgcgt ccggtttcca
    30781 gttccatcca ctccgcggtg aagacgccgc ttgcgcccgt cagattgacg gtcaccggtc
    30841 gagtaggctt ttcagcgccg gactcagcca ccgagtcgct ggcaggaata tagatcaaat
    30901 actcacgccc ccgatcagcc aagcagtagc cggttgaagc cagctcgctt tgtggagtta
    30961 cacgcttcaa gttaatcctg tccgcccacc ggcgcgtctg ccccatcgcc actcgcgcgc
    31021 gctcatgttg cggttcgttt agcaccggct cgccgatggt taactcatgg accgcgtcaa
    31081 agggatccat atagatgggg ttataccccc gcatgaagct ttcccagaca tattgctgat
    31141 caccgccgat gccgtggata tgatccgtat ccagcacctg cacctgacgt ccatccgtgg
    31201 ccgggggatt tcgcgcatat ttggtatacc cgccgggaga gatccagtcg gcctcgctct
    31261 caaacagggt gcgattgtca ccgccgccaa actctcctag ttgaaaaaac gtcatgccga
    31321 ccggatgcgc catgaagcct cgctcacgct catagcgctt gatgaagcta atgaaatggt
    31381 attgccagct gaccgactcg ggcgtggcct cgttcaccac ctcatacaag acattgtcca
    31441 gatcgtgcag cgtctccaac acgtgccgca cataggcttc ttgaatctgg agcactggaa
    31501 ctcctggcga tcgatgaacc tccaaaccca gctcgtcgcc gttggcgtca ccgtcgatgc
    31561 cgttcacatt gttctgacca ttcagcggat gcgccttcca ccctcccggc gtcaccacgc
    31621 tgaagccttg aaagaacatc accgaaacat agataccgcg ctgccccgcg tccttcaccc
    31681 tcgctcgaag ccgttcgaag taggccggat tgaagcggga cagatccaat cgtgggccac
    31741 catccagagc aaggcctggc ccggtccgca gatacggaag cggggcgata tccttttcat
    31801 tggggggagg attcatgagc cacccgccgt ccgaatggat cgattcagtg acccacaagc
    31861 gcatgaaatt gtgatgcagc gccgtcagac gatcgagata ggccggatag tcgaagacct
    31921 gcgtgacatc ctgaaaattt tcccaggtat gggatccggt gaggtaaacg atccggttgt
    31981 cagcatcggc aaaatatcgt ggattgtcgg gagactgtcg caagggtccg tgtgcgtgcg
    32041 acgcgtgagg agtatccccg gcccaacact ctacttggcc ggcgccgcat aacagcagga
    32101 cagtcacgag acattgccac aggttggaat accgcaccat cgccctccgt cgagatcaca
    32161 cacgaggcgt cgaaaattta cgccgcagag acgagcggcg cttacagtct ttcgtggagg
    32221 gacctgagac atccggaaca tgccggacga agtgcgttag tggtaggtga ttcgacgctc
    32281 gcggacgtgc gattgcttga ttcgaggctc accatcacgc agttcccacc attgaaaggt
    32341 ctctgtaatc cagttgccga ccgtatcgta ttcataccgg tatgtcttgc gatccatgag
    32401 ccggccggca gcatcatagc tgcgctcttc gatctgacta cctcgctcat cgtagcgaaa
    32461 ctccgaatgc ccttgcctac gcccccgcag atccgacacg gtcttttgct gtacctgtcc
    32521 acgatcgtta tagatgatcg ccacgtcgtg ccccacgtcg aatcgatccg tggtgaccat
    32581 ctgatgtccg tccgcgtcgt accgataatt ccgttcgaat cgctcctgta acagacgccc
    32641 acctccatca taggaataga ggttccgatt cacgacgtgc acggcattgt aatggaggcc
    32701 acgcctgagc cgtcgcaatt catcgtagtc atacaaggac acattttcga aggtcccatc
    32761 cccccacgcc gacaactcgg cagagcggtt gccctgcgca tcataaaccg cgcgagtctc
    32821 cttcacgagt tcgccttgcg catcacggat catttcggcg atacgtcttc cctccttgtc
    32881 atagacatag gtaaatcgca gcggccataa gccgtgagat gtatccttgc cgccctgaat
    32941 gcgttcgaga atacgcccct gaggatcgta gtagtcggtc tgcgagacga gactgtcgat
    33001 cgtttctacg gactgaacgg taccgatcaa tccgtcttct tctcgagccg tgagctgggc
    33061 agagagggca ggcggtggcg cgcccgccat gacgagggtg aacaggcaga agaaaagaat
    33121 gggcggagaa atcaccgccc gattccatcg ccaccacatc ccgaatgagt ccgagacatc
    33181 gcgcagccgg gatcgggcgg acaggacggt cggcttccta gtcgacggaa gccagcgagc
    33241 cgagaatatg gtatccccca tcgacgtaga tcacctctcc ggtgatgccg cggcctaaag
    33301 gactgacgag gaacaacgcc gtgtcgccga cttcgccttg ctcggtcgcc cgccgaagcg
    33361 gtgcgaacgc gcgatggtgg tcgaccattt tgctgattcc cgatactccg cgagccgcca
    33421 gcgttttgat cggtccggcg gagacggcat tcacgcggat gttcttgggt cctagatcgt
    33481 gggcaaggta ccgcaccgtt gcttcaagcg cggctttggc gacgcccatc acgttatagt
    33541 gcggcacgac ccgctcgctg ccgagatagg tcagcgtgac gactgaaccg ccgtccgtca
    33601 tcaacggcat cgccgcccgc gtgacggcga cgagggaata ggcgctcacg tcgagggccg
    33661 tcgcaaatcc ctggcgggtg gtattcacaa attccccggt gagttcttcg cgcggcgcaa
    33721 aggcgagtga atggacaaca aaatccaacc gcccgagttt ctcggcggct tctttcatca
    33781 tcgcggcgat ctgactgtca tctccgacat cacacacgca ggccgatgcg cccggcaggg
    33841 tctggaccaa ttcttcgacg ttttcgcgca atcgttcatt ctgatagcta aacaacatct
    33901 gcgcgccttc ccgcgcggca gcctgcgcga tggcccaggc gatactgtgc ttattggcca
    33961 caccgatgat cagtcccttt ttaccttcta gcaacatacc ggcttgtgct ccttcaatga
    34021 gatgacacct gattgggggc tgaacatacc atgattcgcg gatggagtga atgaccttcc
    34081 caccatccgc catcgccgcg ttgcgcgctt ctctgcacgt tccgtcgctc tctcagtctt
    34141 tacctttgcc tcacgcaagg tgatgtccgg atggccatag attgcgcgca tcgaacgatg
    34201 catcttctga ggtgcgcgtt ctgcgaccat atggctatcc agacgccacc tacaacttct
    34261 ttcgcacctc tttccaatcc ttcgcagcct tggctttgcg aggcaattcg cccttgggta
    34321 attccggcac caccacgact tccatgcggt gctgcagcag catatccgca aatcccctcg
    34381 tgcgcccgat gccggatttc atcgtcgtcc atccgtcatg tttcaacgca tcgagggtcg
    34441 cgttcagtcc ccctgatgtc ggcgttgcca cgaaccccac cggaaactga tgcgcttcca
    34501 accagcggcg cacctgtcca ctggagacat ttcccatcgg cccctgctcg ttgttcgaga
    34561 cgtagaccac gttgtagtag tactgggtca gtcgggagag ttcctcggca gcctccggaa
    34621 ccggcgtcgg cacggtgtca tccgttaccc ctatcccagg cgatgcggca ggagttgccg
    34681 gggcgggaga ctgctggatg agcgcgtcta attcgaccac cacaatcggg cgccggcgtt
    34741 cccacacaca cagggtcgcg tcgacttttt ccgccgcaac gggagcatcc ggatcgatcg
    34801 acacactgat gacattagtg cctcgcattt tcgccagata atcgaacgcg gcccggccgc
    34861 cttcattggt tttggccgtc gcaacgtctt ttccgtcgat ctgcaggtgc agcatcagac
    34921 cgctcaacgg cggacctgcg gccgtcacag cgacctgcgc ttctatgcga accgaatgat
    34981 tgggcaaggt cagcgcatcg tgcaggatca aggtaccgga gaccttgccg gctgcctcgc
    35041 cggtcgtcgc cagtgcgagg gcggcacaga gaagcacacc gaatatgcgc agtcccagac
    35101 caacaacttc tgccatggtg cgggtggtct tctctggtga gcagcacatg cgtgatcagg
    35161 agaacaatcc gcggtcgcgg ctgacggtga gataggtatc ccccatggag aacaggacga
    35221 ggccgagcaa cggcacgagg gcaaacgcaa accagttcgt ccacaggatg atcagcaaca
    35281 acccggccgc gaggagcggc ccgccgcgct tttcaccgat atacaggtgt ccaagtcccg
    35341 gcatcagaga caacgcagct gcacggagag catgggatgg tttttccagc ggatccatag
    35401 aagatacaga ggagtcaacg acgcaatcgc tgtggtccga acagaagcta ggccttcaac
    35461 gcggccaaga cctcttccac atgaccgggc accttgacct tcctgaagga ggccttgatt
    35521 cgacccgttg catcgatcac aaacgtactg cgctcaatgc ccatgtattt ccggccgtac
    35581 atgctttttt ctttatagac gtcataggct gtgctgaccg ccgtatcttc atcgctcaac
    35641 agcgcaaacg gcaatccgta cttggcgata aacttctggt gagaagtttt gccgtccttg
    35701 ctgaccccga tcaccactgc tccggtcttc ttgatcgccg ccagggaatc ccgaaaatcg
    35761 caggcctctt tcgtgcaacc ggaggtatcg tctttgggat aaaaatagag cacgacttgt
    35821 ttgcccttca aactcttgag cgtcacgagg ttgccgtcct gatccggaag ggaaaactcc
    35881 ggcgcctttg ccccgacttc tactccactt gccatgctac gcgtctctcc ttctccacca
    35941 tcgtttgcgt taccgcgggc cgctaccgat cgtaggcctg ctcgttccgt agggatgctc
    36001 tctcgccggc cccttgggca cttccggatt tccaaaggga cgcaatgccg gagaatccca
    36061 gatgaccttg tccccggggg ccagatttgc cgcctcgatg aattcctgac gggcctgatc
    36121 agtttgcccc aaggcattca acgtcaaccc gtaattgtaa tgcgcctgtc cggagtccgg
    36181 cgctatcttg atcacctgtc cgaagaagtc cttggcctca gccatttggc cggccagaaa
    36241 ggcctgcctg ccctgattat tcagcgcatt cgcatccgcc ttcgccccgt aggccagctc
    36301 gagaggcacg agcggtttcg gcttttcatg agcacaggcc gaacacgaca gcaccaccac
    36361 taccagggct atccagcgac tcactctcat cgcgccacct cgttcatatc attcgccagc
    36421 gtgattaggg cttgacgcgt tttttctgtt cttcttcgga cgtttttcga tacatgacgt
    36481 cccattcggg actgccctcc acgatctgct tcgtgtagga cgccagtttc gcccgcactt
    36541 ttttatcaat ctccgcgtcc agggcgagtt cagcagcgac cacgcgtttg atctctctca
    36601 agaccttgcc gtcgtcttcg gtcagcctgc actgagggct tttctttaac gtgcccagta
    36661 tcaggtgggc aaggtggctg gctttgtcat cactgatcac gattacggct cccgtctcga
    36721 gtggcacggc cactcataag atgatcccgc gctcacgcac cagtttctgt ttgatcatgg
    36781 tgaacatctt ttgatagtcg acctgccctt gctcgatctg gcgttcatat ggctgcatca
    36841 tccgacgcac ttcggcattg aggcggtctt cgaccatcag ctcttccgtc atcgtgtgtt
    36901 ccaacacttc gcgaagagcc tttcgatctc cggtcacctc gagaaaattc aactcctgca
    36961 gtcgtgcgac taccgaatca gccatatgat gaacccgctc tttcgccaat ctcatggcaa
    37021 tcgctccccg ttcgccttca tcgctgaacc ctacccgacc ggttgtgtcg gctcgattcg
    37081 ttccacccgg atcatactgg cgttcatggc ctgccgtaat gtcgccgcat cacccaacac
    37141 ccgccccaca acgttcaccc ggtcggcagg cacagggtcg gcgcccatgc tcatcggcgt
    37201 gcggccaaaa aagatcgcca gaacctggcc ggctccccag aatgccacgt cgccgatttt
    37261 gacttgcgtc gtcgccgttt cgcgatgatc tttcacgccg ggaattttaa aatagaactc
    37321 ttcgccccac acattcaacg gcccttccgc cggcaaggct cgatacagtt cctcggccgt
    37381 tcgcgtcccc ttcaactcgg cctccagttg gaggccgccg accttgattc gaattcgttt
    37441 catcgtgccg ttcgtggtca tgatccctca ttcacaccgt gatactgtgc gcaacaccaa
    37501 atgacgaaac tccgcaccag tcgcggactc taacttgttt atcccctgaa atcaaggtta
    37561 caatcccggc cgcttctatg ctgacatcca cgtttgtcca tttaaaaggt atcgggccat
    37621 cgaccgagcg acgtctgtgg gaaagcggca tcgccgattg ggcgaccttc cggcgggaag
    37681 cccacctgac cggtatcgcg ccggcgcgta aagctgccta cgacgccgag ctggcccttg
    37741 cacaggacca cctcgacctg cataaccccc ggtatttcgc agattgcctc cacacccggg
    37801 atcattggcg attgtttcag accttcgggg cccgcgcgct ctacctcgat attgaaacca
    37861 caggactctc ggcccgcgag ggacaggtga ccatcgtcgg acttcatcgt caggggcgca
    37921 tgcggacgtt gatccatggc caggatctta ctctagaggc cattcaagag gaactcgatc
    37981 aagccgacct gctcatcaca ttttttggaa cggtcttcga tatcccctat ctgcaaacct
    38041 gctttcacgg cttgcgcgtg gcgatccctc atttcgatct ctgttttgcc gcccgacgcg
    38101 tcggcttgca aggcggcttg aagtccatcg aacgagagtt ggacattgca cgggaatcag
    38161 acctgctgca gctcgatgga ttggaagccg ttcgattgtg gcaccattac cgcgcaggtc
    38221 acgacgaggc cctggatcag ctggtccgtt ataatgcagc cgacactcaa aacctggagc
    38281 ccctcgcagc ctgcctgtac gaacggctgg tcttgcgata cgggcctcag tcactcggtc
    38341 tggtcggata acgtggcgct cgctcaaggc aagtgggact ggacaggcta cgggcagacg
    38401 gaccgtggac tggtccgctc gaccaatcag gatgcctttc tcgtcgacaa ccggcgccgc
    38461 ctgtgggcag tcgctgatgg catgggagga catcccggag gagacgtggc cagccggatc
    38521 gtcatggaca cgctcgccga tttttcacca agcctcattc gcaattccga caacctcgac
    38581 gaggaccata ccgagcagca gtcttttcgc ctcacaacta tggcggaaca ggctcatgat
    38641 gccgtgcgcg cctatgctga tcgtcacccc tctgtctacg ggatgggaac gacgctggtg
    38701 atggcccatc tgatgcctct gcccgcccct cgactccttg tgctcaatgt cggcgacagt
    38761 cgggcgtatc tcgtccgggc gggacgcatc acgcagttga cccgagacca caccctcatt
    38821 gacgactatg tgcgcgaggg ccgcctcacg cctgagcagg ctgcccgcca cccggaccgc
    38881 cacgtgctga cccgtgcgat cgggcttgaa ccagtcctgc agatcgatct ctttcctgtc
    38941 gacgtcttcg tcggcgatgt gctgttgctc tgttccgacg ggctcaccaa aatgctgccg
    39001 gacgaacaca tactcaaaat tgcacagtcg catagagaca gtccctccct cacgacccag
    39061 gccctgatcc aagcggcgat tgataccggc ggcatcgaca acatcaccgt cgtggcctgc
    39121 gccatgacca gtctccccga tcagggaaat atccattgac agtgcctcaa ggcaccaggt
    39181 agcctcaaaa ccatacatcc acgaggtaag gcctcagagg cctccatagc tcacttctaa
    39241 tgaacatgga atcgcattac tttcgtccac tcagtctgcg agtccgaacc tggctcctgc
    39301 ttggtccgct cttgggaatg ctgtaccttc cgacggcgcg agccggggaa ccccacgatg
    39361 cggagaccac cctgcggagg cttgttcagg ccaatgccga acgtgatctg gccaccatgt
    39421 ccaaatatat gtcacacgac gtggatgcca tcgggtacac gattgacgga cgcaaataca
    39481 tcggatggtc ggatctggcg gccgatatgc gtagcgagtt tgattcggtg gtcaaactgg
    39541 aaattccgat caccgatctg cgcatgtgga acaacaaaga tgtggcctgg ttcgcaatgg
    39601 aattagacta catccgctac agcgatccgg cggatctcgc tcaacgcatg gtgctccacc
    39661 tgcgtgaaac cggagtgctg gagcaacgga acggccggtg gattctggtg gcctggcatg
    39721 aatcgacacg ccatcatgac agcggaacgg aacagaccgc ggccaacggc gcggcactgc
    39781 ttcatcgcgc ctcggatgcg cagccggccg tcccgaacgg cgtcgactta agtggtgaat
    39841 gggaagtcac ggaaatcgaa gataataaga aatacgtcgc cacgctgaat gcccagggca
    39901 ccggtcccta cacgtggcaa ggcggacagt tttccaccac cagcttcaac gatcgacgct
    39961 ggcagggcac gtggaaacaa acgggaaacg atcgcgaggg tgcctttgaa gtcgttctct
    40021 cagaggatgg cacggaagcc aaaggcatct ggtggtatgt gcgtgtcggc accagagaca
    40081 atatcccgcc ccggcaacat ggcggcagct acctgtggaa acgactcacc ccgcctccga
    40141 cagctcctta acggacaggc ggtcacgccc tacaacgctt tacacacact catggacggc
    40201 cggtctgtta caacgaggag gggcctatga atcgtgcgct tctgttagta gtcatgttct
    40261 tgggcgtcac gagtttggcg tatgccgacg gcggacatga tcatcacgct attcctacca
    40321 tgccgagttt gggggagacg aaactgacga tcgatgagca gaccgtcacg ctcacgttcg
    40381 gccccgtcga tctgccgacc ggccatgatg gcgatctagc ctccagcatg ccgaagaagg
    40441 tgttccaact gccagaggac acctacatga tcggctacaa atccgaggtg ttcacgaaag
    40501 atggcaaacc ccttccgaaa aattatctcc atcacatcct catgttgaac aatgacaaac
    40561 ccagcgtctc atgcgatggc gagccgttgt tttttgcggg ggccggactg gagatgacgg
    40621 aagcccgttt cccggacgga tatggagtaa agttgggcaa aggccaaaat ctcatgtccg
    40681 ttgtggcctt ttatcacaag gccccgccga cgaaagacgt gatggcgcgc ttcaccatgt
    40741 acgtcgctcc caaaggggcg aaggtgcagg agatggacgt ctaccaggta ggcgtgaaca
    40801 ttgtgtgcta cagcaagttc gcccaacggg gcccggacca gacggacgaa gggatcgaaa
    40861 tcaagtcggg agtctcggtc ctttcggcgc cgctgaaatt caacatggat ggctgcgtca
    40921 aatttgccta tccccatggg catgacgaac ttctcatggt ggcgctcgag aataagactt
    40981 caaaagagac cctgctacgc accgtccccg aggtcgatgc cgacggtaca ttccgggact
    41041 ttcgaccgca tcaggtttat cgggatagca aaggattttc ggtgacgaag caggatgact
    41101 acgaaatggt gatggttcac catcatcttc tgcaaaaccc taacatccaa catggcatgg
    41161 gtaactatct gctctatatg acgcctggcg cctgccagca gtcgtcgacc acggccgcgg
    41221 cccactagcc aacactgtca ggcacccacc acaggacgcg gctgtggtgg gttcagccca
    41281 atccaggctc cgcaccgact catactaaaa cctgttccaa tcggaacact tctccaattc
    41341 agggtctccc tagctttcgt cagtcgctca gatttcaatc gttcctcccc taccgaagtg
    41401 gaaccgcccc ctcgggcggc ggactgggat tctcaagaag gtgtatgata ttgaaccaat
    41461 catgcagtcc aatcagctgg ggaaggacag atttgtccgc ctgagaggga gagcatgttc
    41521 atcactgtac ggtggtgcat tccccggggc actcacagcc tcggggcacg ctgaacgcgc
    41581 ccctgcatga agaccatgag atcttccatg tccttgcccg aaccctcgct gcatatcctc
    41641 ctcatcaatg agaatgccga cgaaatcaag ttggtgacca ccagcttgcg gagctttttt
    41701 gtgggctgtc gcatcgaagc cgggtactcg tccgaagatg cccttgcatt cacccgccag
    41761 ggcgactggc aaatcattct cattgatcag gatctgagtc cggaacgagg tctcgatctg
    41821 ctcgcgcgcc ttcgtcgcaa tgcgccctat gcggcaatcc ttcttcagac gaacgagagt
    41881 gactcacaca cggccgttca agccctccag cacggagccg actttttgct cttcaaacga
    41941 tcgcccggat ttctcacgga gctcctcttt tccgtacagg aagccctcga aaagcgcgac
    42001 ctgcaaatga agctggacca tacgtttcaa cgccacctcc gcgtgatcga aacgttgagt
    42061 gatctcttgt acgaactcga tgcggacggt cgtttcgtgt atgtgagtcc aaccgtcacc
    42121 gcaatgcttg gatacacggc tgaggaactc gccggtcggc actactctct cctgcttcct
    42181 cctcaacagg aaccaaccgc gcgttttcga ttgaatgagc gccgggcagg cagccgttcg
    42241 acacgccggg tggaactcac gctctaccgc aaagccctcc ccgaggctcc atccgtaccc
    42301 atcatcgtcg aagtcacagc gaaaggcctt ttcgactcca cgaatcgata cttgggaacg
    42361 gtcggattgc tgcgggatct atcccagcaa aaggcccaac agaaccggtt ggcggaactg
    42421 gagtctcgtc tccaggagac cgatcgtcaa ctcaccctct cccgcgaggc agcccgggtc
    42481 tcccgccaac tgcagcaacc ccttacgtcc cttcttcagg actcacaacg attgctgacg
    42541 tcgatccaac acagccggtt cgagcaacat gttgaaacga tggtcgctca ggcttcgcaa
    42601 gcgagccagc tgggacatca attggccgaa gcgatccatg ccccgccgac agaattgatt
    42661 cttcttgatc tgaatgaggt tctccagaca gttgcccaga cgacgcacgg tgagacggaa
    42721 gattcccaat ttctcctcac cactcagttt gcggcgaacc ttccactcat tctcggttcg
    42781 cgtgcggacc tggaagacct cacccatatc ttgctcagct atgcctgccg atccacacct
    42841 gacaacacac gtcccagctt aaccttgcac acagcagtac tcgccgtgga gacttcagga
    42901 agtcctgggc aggaggtggc atcgctttcc cggccgcttc gtacgtatgc gagctttacg
    42961 atccggggaa gtgccagctc actccgtctg cctccggaca gccaatcagg ccacgatcgc
    43021 tcggcagtga gtttattcca ggcacatcgc atcgttcagg tacaccgggg tgccatcgaa
    43081 gtcgaacaca caccagaatc cggattagcc atcacagttc gcatccccgc ctcggaagac
    43141 ttctccctga ctggaacacc ggcaacaaca atctcgccgc ccccaaccga cattcacaag
    43201 cctgggagag acacctcgca acgccctgaa cgtcgccggt cagagcgcaa gccgttttcc
    43261 ctccctgttc aattgtcgat cggaggcacg accctacgcg ggctgttacg caatatgagt
    43321 acggaagggg cgctccttac gatccgagga atggaaagtt cgatccatct gcagcccgcc
    43381 tatgtcgtga tcaagacccc ggtcagcttc cttgaactgc aaggagtcgt gcatgagcgg
    43441 ccctccgcat ccaccgaatt gtcgttgccg gccattaagg attttgtgat ttcctttgct
    43501 ctcgccgtcg aacatgagcg caatgtactc agatccttgc tcgagggcct gcaggagggg
    43561 tccccgcaga tcacattcga aggactgatt ctccccctgc ccatagccca aacgatccag
    43621 caccggaacc tccttccact tcgagagatg ccggaagacc ggcgggaagc cgctcgactg
    43681 agcgtgtctt gccccgtata cataactgga tccgacgcgg cctcatcgcg tccgttcggc
    43741 aagatcatca atctcaatct gaacggggct tgcatcgaac tgccgacaga ggtcgtcttg
    43801 accgaacact cacgggtcat ccatctgaac cctctggagt cgatcaccat ggtgcccggc
    43861 acctcccaag caccagaggc cgaacagcca ctgacggcac agattctctg gaacgattcg
    43921 gcggaaagga ggctgaaact ccgcctcgca gcaacgggac atcacctcca gcagatcggt
    43981 atacgctttg atactctgtc ccccgcacaa gagcagagac tgcgtagcat catggaggct
    44041 gaattccggg cagagcagca tctggccgaa accccgtcgg atgcccctgt tctcaccgtg
    44101 tcacacacgg tgcggaaccg tgaaggctat acgattgcgt tgcggcatga cttccctaca
    44161 ggaggcggca gcgagcatga tccgctggta ctcctgtgcc ccggatacgg cacgacgcag
    44221 cacgcctatg tcgcgttggc ttatatgctg gctggccgcg gctttcatgt ggtgcgttac
    44281 gatcacagcc gccatattgg cctcagcgac ggagaccctt cgcaaaccac attcacctct
    44341 cttgaggacg accttgatac agtgttgacc ttcgtgcagg aacactggcc ggaaagaccc
    44401 ctgacgatat tggcgccgga ccttcccgct cgaatcgcat tgcgcagaca ggattggcac
    44461 aggcggattc ggcgccttct gcttctcaat ccgacactcg acctcggaca gtgcctgcaa
    44521 accctccacc accgagatct gttacaggaa cacctaaacg ggacgtgcct gggcctggga
    44581 aatttactcg ggattccgct cgacatcgac cattttttgg ccgacgcagt tgccgcgcag
    44641 tatacggatg tcagggtcct gcacaatgaa ctggcccact gtgaaacgga ggtagtcgtc
    44701 ctcaccggcg gacaagatgg ccctgactgc tcgatcccgc ctcctcctca acctctcgtg
    44761 aatgacctcc tctcccggct tggcgttaaa ggcaggaaag tactgttgcc gtcgtctacc
    44821 ttgacatcag gccctcagcc accttcgact ctacgcgcaa gttggcaaca ggtgtcacac
    44881 ctctgccaag cgggacaggc acttcacgaa ccaggaaggg tcatgcttcc gtcgctcttg
    44941 cttgcaacat ccacacgtgc ccggttcgaa cgcgatcagc ttcgtcataa acacgccatc
    45001 ggcacgacga gtaacgaacg actttggacg gcacacacgg aactcacccg ggccctggat
    45061 gaactgccgg cacattggca gtctatcgat caactctatc aactcttgca gcctctcgac
    45121 ggcaatgtcg ccctcctgga tatcggatgc ggcatgcatt ccttcgctcg gttgctgcta
    45181 ctcaatcttt catatcgcct gcgcgctcaa acctggcatc ataaccaccc actccgatat
    45241 gtcgggctgg atttttcctc tgcttcactg catgctgccc gagcctcgac aaaagaagcg
    45301 ctgcaacacc ttgacgagct cttttcagga cggatctctg caccgacgcc cgtcacccag
    45361 caatggatcc tcggtcgctc ggcagacgcg ctgccatttg cggactgctc attcgatcgt
    45421 gtggtggcaa atttctcgtt gagctttgta cgctcacccg tgcactcgct ccgagaattc
    45481 ttccgcgtgc ttcggcccgg cgggaaactt atcctgaagg cttttacccc cgccacagat
    45541 ctggctcgcc tgtacagacc ggccttacac gaaatcggca tcgacgcatt ttcaggagcg
    45601 cctcgattgg ccttgcaccg tatgggacag tgcgcggtag cactacgtac agggcaatta
    45661 cacacgtttg aggaagagag tctgaccaca ctgatcgcac gcttcacacc gcttacacct
    45721 agacttgttc gtactctcgc cggacagatt ctgctcgccg ccgctgaaaa accggattcc
    45781 tctagctgaa acaatagagc gcatatatac ttcactcact agcataaaac atgcagtgag
    45841 tcaggcgaca cctggggaaa cctggtgtcc ggaatgatat tcctcccacg ggactatgac
    45901 gctccacgcc aacatccggc agatgcccgt cctttcagct tccacggaca ttgtcagact
    45961 gatccgtgac ttcgctgaaa ccctgggatc ggcacgagac ctgtcatgtc tcggacattc
    46021 gattgtgacg gctctttcgc aatacactcg gttgcccgaa gtagccctgt ggattcgcga
    46081 gtctacaggt gaacggtatc gtctccttgc atcgtatgga cacaaccatc cctcttcatt
    46141 accatcgact ttgacgggtg atcacccgct cgttgcaggt ctctccgcca aaccctacgt
    46201 atgtcatggg agcgagaatc cctctaaacc gcttgggttg gcgatggcga cggcccgcgc
    46261 gagcctgtgc ctcggactac gaaccaaagg gcgactgctc gggatctgca tcctggggcc
    46321 tctcgacacg aaagcgcagg tcggcgaaga actgctcgcc atcgttgacg cgattaccca
    46381 catcgcaggc aatagcctcg atcattacct gacgcaagac gacctccgcc gctcatccac
    46441 attgatgaga cggacggatc gactccgttc tctcgaaatc atggccggcg ggtttgccca
    46501 tgaaattcga aacccgctca cgtccatcaa aacattcgtc cagctggccc cccagcgaca
    46561 gcaggatccc gtcttcatca aagaattcag caaactggct atcgaagaca tccatcgcat
    46621 cgaacgactg cttcatgaga ttctcgacta tgccagttac atgacgccgc gccccacgga
    46681 agtggacctg aacgatctcg tcgcctcttg tatcggcttc atctcatcga gcgccgcgca
    46741 tcggggaatt caggttcaga cgcacctgtc gccgcagctt cctctgctct tgctcgatcg
    46801 ccagcaaatc aaacaggtgt tgttgaatct gctcctgaat gctctggatg caatgcccaa
    46861 tggcaacggc atcattcggg ttcatacgcg actcctgtcc gaagccggcc gccccccatg
    46921 ggtacaattg caggtcgagg atgaaggatg tggtatcgcg cccgatcatc tcgagcacat
    46981 tttcgatcca tttttcagca ccaaacattc caacagtgcc agtgaaggag ccggcctcgg
    47041 gctcaccacc gcgcatcaaa ttgttcgaga gcacggtggc accctgatag tcgaaagccg
    47101 cctaggagcg ggctccacgt tctccgtgaa tcttcctgtt cccgattcag cgcccacagt
    47161 ctcagcagca cgggagtaat atgaaaaaac gagtcctact tgtcgacgat gagccgcgtg
    47221 tccgggcctc gctgagaacg gtcctagaac ccacgtatga aatttttgaa gcggcggatg
    47281 ctgctgaggg ccttcgcagt tttaaacacg atgccccgga cctggtcctc ctggacgtca
    47341 tcctaccggg agcggacggt ctgtccgcgc ttcagaccat gcgaaccgag aatcgagccg
    47401 taccggtgat tatgctgacc ggcaccaagg cagtgaaaac cgctgtcgat gcgatgaagc
    47461 tcggagccgc cgactatcta tccaaaccgt tcgacgtcga ggaacttcaa attgtcatcg
    47521 aacgaacctt gggtaagcag gaactggaac aagaggtccg gcaattgcgc gctcaggttg
    47581 tgcagcgcta tgccttccac aacctcatcg gcaaaagccc cgcgatgcag gaaatctacg
    47641 ccaaaatcga acaagtggca gatagccgca ccaccgtcct ggtcaccggt gaaagcggga
    47701 ctggaaaaga actggtcgcc aaagccatcc attacaacag cgctaggcgg gaacgcccgt
    47761 tcgtcgccct caattgcgcg gcgttgcctg aaacgctcat cgaaagcgag ctgttcggcc
    47821 atgaaaaggg ttcgttcacc gatgccacgg cacgacgagt gggacagttc gaattagcgc
    47881 atacgggcac gctgttcctg gatgaaatcg gcgatctcag tcccgcaaca caagccaagc
    47941 tcttgcgggt actgcaggaa cgggaattta cgcgggtcgg cggggtgcaa tccatcaagg
    48001 tcgatgtccg gatcgtcgcg gccaccaaca agaaccttga tgagatggtc aggaaaaacc
    48061 agttccgcga ggacttgtat taccgtatca acgtgattgc gctgtacctt ccgccattac
    48121 gtgagcgcgg cgaggacatt gcccttctcg cgaagcattt tctttcgaaa cgcattgagg
    48181 aagagaaacg accgtctcag gaattcacca aagacgccct cgaattgatt tcacattatc
    48241 cgtggccagg caacgtgcgc gagatggaaa atatcatcga gcaagccttc atctggtcga
    48301 agggctccac ggtgatcacc ccggagcatc tcccgaatat tctccggact gatacgcgct
    48361 catcgtccct tcgggatgac accctcgccg gacgattatc cctggaaaag gctgtcatgg
    48421 aattcgagcg tgaaattatt ttagatgcgc tcaagcggac gacatacgta caaactcatg
    48481 cggccacgat gctgggcatt agccgtcgca tgctgaaata tcgcatggac acattgggca
    48541 tcagccgacc tgatagcggc gggggaaatg ccgaggcttc actgcctcag gaatgacaca
    48601 gactggcaca agagtctcgg catgtgtctc atactttttc acgtgccggt cgccccgctc
    48661 aaatatcttg tggcctgaaa tagttagacc tgctatatat tgaccgttta cggtatacgt
    48721 agacggtaca ggttttgcgt tttatctttc ctgaagttcg caatgcaccg ggagcgctct
    48781 ggatgggaac tcaggcatca actgaacaga tcagcataaa accgtcggtc ctggtcgttg
    48841 acgacgaaac aggcccgcga gacgcgctga aagttattct tcgccccttc ttcacgatcc
    48901 actctgccga caatgcgaaa tccgcgctcc gggttctgaa ggaccagcac atcgatctca
    48961 tcaccctgga tcagaaacta cccgaccgtc aaggtatcga cctcctgcag gacatcaagc
    49021 aggattacgc agacatagaa gtcatcatca ttacaggcta tggcagcttg aaatcggcca
    49081 tggaaggcat ccggcacggc gcggccggct acctgctcaa gccattcaac gtcacggagc
    49141 tgatcaccct gatcaaccag accctggaaa agaaacagcg actggactac ttacgatcgt
    49201 tcctgaaaac atcgacggcc ttgtggggca ccgaacagga agcggcagaa gcgtggaaag
    49261 atctgcaatc gaagtacttc gcgatcggca attcctcaac ggacgcgacc ggaactgcag
    49321 cggacgtcac agcgctcatc ccactgttat ccgacctgct cgaagccaag gaccggcaac
    49381 tgctcaacca ttgcagccgc gtgagtttct atgcctcact tctcgccaac cagctcacgc
    49441 tgcccctgcc cgaccaaaag gctctggcgc tgggagcatt tctacatgat atcggcaaga
    49501 tcagcctctc caactacaag tattcagcgg atgatgatga gagcctgaag acgaacactc
    49561 atgggaaaga gtgctttgag acaggggccc ggatgctgct gccacttgga ttccaagcgg
    49621 aggtcggaca gatcgtggcg tatcatcagg agcgcttcga cggtgtgggc aatccacacg
    49681 gtctagaagg cgaaggcatc ccgcttttgg cacggattgt cgccatcgcc caggcgttcg
    49741 acaacctgac ggtggatatg ccgggacacc agccgacatc catcgatgaa gccattcgac
    49801 agattctgct gcaagccgat acctacttcg atcccaagtt gaccgagctc ttcgcgcacg
    49861 tggtgcgaga atgtaagtct tccctgccgg cactcgcgat cgccaaaacc tcgcccacga
    49921 actaaccggc cgttactgcc ggtccttcgc gctcgcaatc atttcagccg ccgccgcgcg
    49981 cgccttcttg gtcaccgtca cgccggccaa catccgcgcc acttcttctt cccgttcatc
    50041 caggtccagc aggcggactt gtgtcatggt tcgattctgc cgaacccatt tttccacgag
    50101 ataatgcgca tgcgcttgag agcccacctg aggaagatgg gtaacgcaca acacctgatg
    50161 gtgccgactg agactcctca gtctcacccc cattacctcg gcaacggcgc ccccgacacc
    50221 ggcatcgact tcatcaaaaa tcaggacagg cacctgatcg ctctcggcca aaatcgtttt
    50281 taatgccaac atgacacgcg acaattctcc gcccgaagcc acctttgcca gcggtttgag
    50341 cggttcccct ggatttgcgg agaacataaa ctccaccgtg tcctgtcctg tctgaccgta
    50401 gggcgcgtcg ccggacagac gcgagacctc cacgctgaaa cgagtccgct ccattcggag
    50461 cccccccagt tccttcatca cctgggccgt tatttttttg gccgcgtcga gccgcttacg
    50521 agatagacgg tccgccacat cgcgtaggct gagtacagcc tgctcgaccg cacgagtacg
    50581 ttcctgcaga tgggtctccg catggtctaa ttcagccagc tgtgcacgca gagaatcctg
    50641 ccaagccagc atcgcatcca acgaaccgcc gtattttttc gcgagccgat gcaaccgatc
    50701 cagccgctgc tcgatctcca gcaatcggct gggattggcc tccacatgat cacggtagcg
    50761 gcggacctgg tcagcaagat cttttaacgg gacgaccgct tcatccatcg tcttgatcca
    50821 ctcgacagtc gtggcgtcga gcgactccat tttggcaagc agcttcctgg caccagccaa
    50881 tagacttaag actccctggt cacccgcgta gagcagctca tggagctggt cggacaattc
    50941 tccgagctgc tggctgtgca tcagccgtgg acgctcctgt tcgagtcgtg catcttcgcc
    51001 tgcctcaatc cccgcgtccg tcatttcctg aaattgaaat ctgaggaggt cctcccgctc
    51061 ccgacgctgt gcaatctgag cagtgagcgt ttccagatca cccacccggt cctgccaatc
    51121 ccgatacgca tgctgatatt cctgccggag cggctgaaag ccgccgaacg catccagcgc
    51181 atccatctga gcggaaggtg acaacaagga ttgctgctcg tgttgtccgt gcacatccac
    51241 gagggcgccc cccagttctt ccaggagatg gacagggcac aggttgccgt ttagataggt
    51301 tcgattgcga cctgtgcgtg agatgatgcg ccgaatgaga atatcggtct cgccggaccg
    51361 tgcaaagcct ttgttgcgca acaatgcaag gagcggatgt tctggtggca gatgaaaggc
    51421 cgcttccagc tctgcttctt cggcctgcgc acgaatgtgt tctgcggaag cacggcctcc
    51481 gacgaggagc gtcacagcat cgatgagaag agattttccg gcgccggttt caccggtaaa
    51541 tacaatgaaa ccggcgccaa aggtgacggc cagctgctcg atcacaccga aattcgagat
    51601 gcgcagctcg gtcagcatgc cgctgagagt ctggcgctag gcggcgccgg aatgttgagc
    51661 aaggagcgta tcgatgatct ctttgaactg gcgtttggga cgcgcaccga ccagccgttc
    51721 tacggcctga ccattcttga agaacaagat ggtcggaatg ctcatgacct gataacggcc
    51781 ggcgatttcc gggttttcat cagtattgag tttacgaacc tttactttac cagcatattc
    51841 tgtcgccaat tcatcgacga tcggagcgac catctgacag gggccacacc acaccgccca
    51901 aaaatctacc atgaccaatt cggatgcctt catgacatct tcatcccacg ttgcatcagt
    51961 cgctttcaac gcatcgcctg ccacaattgc ctccttctct actcacaaac tgctgtctgg
    52021 ttcacgtcct gaagcggctt caggtactgt tggtcatcct ataacatggt tttgcggaga
    52081 gtcaaatcac ggtgccgccc attgccactg cggcggtgca gccgaccacc aatcatccgg
    52141 tcgggcctgt ctccatgcgg tttgctgctg ccacagttgt ttgaccagag tctcgtccaa
    52201 gcgcccggct agagctgcgg tgacatccgc ctgccgttgc gcagcctgct gaatcacctc
    52261 tttggctatc cgagccagga catccggcgg atcgagcaaa tcctccacgc gtcctgtaga
    52321 cagattcaca tagagatgtt cgactcgaac ccattgatcg gtgtttgcta aatactgcaa
    52381 gtagccatca agagcatgat aggcataggt cagatacaga taactccgag cggacggcct
    52441 atccttcact tctgcctgac acgcctccag cgcttgtcga taatctcccg ccttcaaaaa
    52501 caccgcggcc cgttcgagat gtgccatgtg ctctgtggca gaaagccctg acgggtaaat
    52561 ttcctgcacg gcgatggcta gcatcagaag acaggtcgtc agaacggtac gccggttgag
    52621 tcgaattcca gctgcacgct tcgtttcgat catgggcgat gctcctgaga gatggaaatc
    52681 caaccgggcg cttcactaat agcgccccat tggcatcgac tgaacaggga gcgcattttg
    52741 tacacgacca tagggaccac cgggagcggc ccagggcaac ccacgatctc ctaccagcaa
    52801 cggaccgagg atggtcctgg ccacaatcgt accgaaattt tccgtccagc caatgcctgt
    52861 catgctgaac atgaaggaat aactctgtcg gtccgggaac tgcaggtagt acagtccgag
    52921 cgaccagcac ttacatggat tttgatagag cgccaccaca tcaaattccg ggctgcgtcc
    52981 gcctttcaca tcgtaatatc ccttggcgcc gatggtccac ccccagggcg tccggaatcc
    53041 gccgccggcc gtggcgaatt gaatttcctt ggtgggggca tagacttcat tgaaggaaac
    53101 cggattccaa atatctccgc ggcggactcg attgccgtca tgactgaacc gttgcccgac
    53161 ctcaacgtac cagtaattgg attgctgcac tcgcaggtcg gtattccact ggctgaacgt
    53221 cccccgatac gggtccaaaa acgcatccac ctgtagatag gaatttgtcg gcggcaacat
    53281 ttgtccgcct gtcacattct ttccgagtgc ttcgttaata gcgggaggac gaaaaaattc
    53341 cggtgccgtg ttcccgatca ccgcacgcat ccacacatcg gacatcttcc ggccctgcac
    53401 atctaccgtc gccggctgca aaggctgtgt cagcgacccc aggaacggca atacgccggg
    53461 agtaaattct cgcgcccgcg tctgcaccgc tcctacgtga taactttgcg ccacggtcag
    53521 atccaaccaa ttgaaactct gcccattgag ctgttgctcc aggaacttcg tacgcagcgc
    53581 ataggtcagc aaatttttct tcggaatgtc gtcgatctga tcgatctgcg caatcttgct
    53641 ttgatcgctg cccgggacgt attcataaat cacgcttggt tcgatcgtgt gaaggaaact
    53701 tcctccttcc ccgcctgtat accgccgact caacttcgag gtcgcatcca tggcggccca
    53761 gaatgtctcg cgatgcagcg gactggcctc ctgaatcccg cgcgtgtaat agacctcctt
    53821 aaatttgaca tgcggcgtca agccaatcac atgccccacg tccatcacct ctgtggttac
    53881 ccccggcatg aagtccatgc gattcaccgc gaaaccctgt tcgcgataga agttcacaaa
    53941 gttcgtgtcc aggttcagga gaaccggcat cccgaatggc gatgtgttcg gcaggacgta
    54001 ccccacctcg ggaaggcgct gaaaggtgtc tgggccgccg gaattcaacg gctgcagata
    54061 ttgtccgaga aaataggcgc ttccgtacgg tagccgctgc gtggccagca acgtcgattc
    54121 accgctgggc gaggcccgtt gcgtacctga attgctcaat tgctggaggt attgccggtc
    54181 tgagacgaac gaggcctgcc cgcgcaccag gagcgtgtcc gtcacctgct ggacatgctg
    54241 gccgctgatt aatccgcgca gacgccgctc atccgaacta acctgatcca ctcccgacac
    54301 attcggcaac ttggtctgct gcaggaaact ggcaaaccac tgcccgctcg atcgccggtc
    54361 cagataatag cgataggtga aatcgctgcc gtaccccaga ttattgtagt acgacggcgc
    54421 gatagtgaga tcctggctgg ggttgattgc ccagaagtac ccctgctgat agtgcaagcc
    54481 gaaccggtta tcgtagccgg gcgtcggaat gagaaacccg ctatgccggt tgctcagcgg
    54541 gtacgtcagt gttggtaccg gaatgatcgg cgtatcgttc gcacagagcc acgcccgctt
    54601 catcccgatg ctctctcccg cattcacatc gagatcctcg aaggtaaatc gccaggccgg
    54661 cacttcgccc tccttggcat cgcaattggt aaacgtacct tccttgatgc ggtagtgatc
    54721 ctccgacaac cttcggatac tgcggccggt aaaaaatgaa ttgcttggcc ggataaacat
    54781 cgacccgttg gcgacgatgc cggcttcggt attcacattc aattccagcc gttcggattt
    54841 gagatcggaa ctcgcgtccc agaaatgcac acggcccgtt gccgtcaacg tgccaggcaa
    54901 ggcattgatc gtcacatgat cggcggtcag ccgtaaattc ccttgggtca ccacgacgga
    54961 tccatccgcc tcatacactt cacgctcttg gagatgatcg atgcgctcag cggtgaccgt
    55021 cagaggctgg tttgtcggtg aaacggtggt gacggtcgat tccccccacg ccgagggcgc
    55081 gaagagcgga aagagaccga aaagaatgga aatggagagg atgcacggaa agtggctgat
    55141 caggcgctgc cacgttgaaa ccgaataacc gtctcgcccc atctcaacca cgaacaggcg
    55201 agagggaatc ccctcgaaga agggccttga tctcgccgac cagcatcaag gagcccgtga
    55261 cacaaaccac gtcggacgca gcggctgaat gcttggccat ggccaaggca tccgaagggg
    55321 tcgtcgcaac ttgcgcaaac ggtgcgtcat gctcaagcaa cagacgaagt tccgacccgg
    55381 tcgcggcacg cggaaggtcc gcctgagtca gaatcagcgt atcgatcaac ggcagcaatg
    55441 gcgccagaaa ttcgcgcgga tgcttatcac gcatcattcc cacgattaga cagagccgcg
    55501 cggcggggct gacacgcttc cattcggata gatattcagc gaggaccgcc gcagccgccg
    55561 gattgtgggc cccgtctacc atcaccgtcg gctcctggcc gacaatctcc agccgcccct
    55621 cccatgccac ctgctgcagc cctgctcgta cggacgtctc cgtcactgtg acaccgcgct
    55681 catgagccag ctcgatcagc gccagggagc aggccacatt gtcgatctga aatcgaccct
    55741 gtaagggaca actgaggtgc tccagccgca gtccgaaacc tgtgtatcgg cagtcagccg
    55801 gcgaggatcc ttcacagcgg aagtcccgat cgagacgaag gacgggagcg cctactgtcg
    55861 aggcgcgctc ttcgattact cgagaacccg gctcaccgat tcgacccagc actaccggca
    55921 ctcccggctt gatgatcccg gctttttcca tcgcaatagc ttcaatcgtc gagcccagat
    55981 aggcctcatg atcgagtccg atcgtggtga tggcggctgc gagcggctcc acgacattcg
    56041 tggcgtcaaa gcgtcctccc aagcccactt cgagcacaac gacatccacc tgacattccg
    56101 cgaaatggtg aaacgcgagc gccgtggtga actcaaaaaa tgtcggagcc agatccggcg
    56161 cggaagccgc gcgcaacaat tcgacgccat ggatgacgcg cgactcggga atcatctcgc
    56221 cgttgacgcg aatgcgctcg cgaaagtcga tcaaatgcgg cgaggtatac agtcccaccc
    56281 gatggccgga gacctgcaaa atcgacgcgg tcatcgcggc agtcgagcct ttgccgtttg
    56341 ttccgccgat gtgcaacacg gggtactgac gatgcggatc gcctacccgc gcgagcagcg
    56401 tgcgaatcgt ttcgagacct aatttaatcc cgtgcagttg caagccatac aaaaattgca
    56461 cggtagcggg ataggtcatc ggggccacgg gagactcaca ttgcatgttg acgtcgaagc
    56521 tcaccaggat gcggaaatga tcgtccggga aggccgtggc gaacacggag cgagaccgaa
    56581 cggtgctggt acctcacgcg agcggacaga gcgggaacat tgccggcaga ctgtgtccgc
    56641 atcccgttag aaatgaccga ccagggtgct gaccgcctct ttcagctgct tgcgctcgac
    56701 gatcatatcg atcatcccat ggtcgagcaa aaactccgcc cgctggaact ggtccggcaa
    56761 ttgttgcttg atcgtctgct caatgacgcg cgggccggcg aatccgatca gcgctttggg
    56821 ctcggcaata atcacgtctc cgagcatcgc gatacttgcc gtcacgccgc cgaaggtcgg
    56881 atcggccaga atcgagataa acggcagttt ggcctcgccc agtttcgcga ccgctgcgga
    56941 cgttttcgcc atttgcatca gcgacaggat cccctcctgc atacgggctc cgccggaagc
    57001 cgtcacgagg atcaatggca tccgcgcttc caaggcgcga tcgactgcgc ggcagatttt
    57061 ttcacccacc acagagccca tactgccgcc cataaacccg aaatcgaaca cgcaaagggc
    57121 gacctttcgt ccgttgatga caccctggcc gatgaccatg gcgtccttcc
//
LOCUS       DAOQDS010000020        56390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_174_length_56390_cov_20.661578, whole genome shotgun sequence.
ACCESSION   DAOQDS010000020 DAOQDS010000000
VERSION     DAOQDS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 56390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56390
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174_length_56390_cov_20.661578"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PK589_06690"
     CDS             <1..920
                     /locus_tag="PK589_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19323.1"
                     /translation="MVHVWPFSIVGRLFYRLRWSKPCYQIINRQARRAFDSIPQPLHP
                     LSVEAVQALNTDGIYRSSVDALVHDANLFHELSHDAQMLLAGQDVQRKIRQRYNPHDA
                     KWYVVRAIGHRAKRPSLPSSFVRFFLHPQVLSIASTYLGLQVRLNYTDVWHNIPVREG
                     EPPISAEFWHRDHEDKRVIKLFVFLTDVDETMGPFTYVRRSQVGGEFGHVYPAIPPIG
                     RYPEQDALSQLITGTPLPSVSCVGPAGSIILCDTSGLHRGGRSVTKPRIVLVGVYTSN
                     AGMDAARYALPRSLPHEHLSPAAKFALYS"
     gene            1052..1600
                     /locus_tag="PK589_06695"
     CDS             1052..1600
                     /locus_tag="PK589_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNJ19324.1"
                     /translation="MGERIQVSGRVVRCVGLLAAAFVAAGWLGAAQAADELPKESVLP
                     AALAGKAVQAALDACKKDGYRVSASVVDRAGVLRAMLRADGAGPHTVDSSRKKAYTAA
                     SLRRATSELADMIAKQPALQALREMNESILMVGGGLPIEIAGEVVGAIGVGGAPGTHL
                     DDACAEAGLDAIGAASKMPAAK"
     gene            1606..2061
                     /locus_tag="PK589_06700"
     CDS             1606..2061
                     /locus_tag="PK589_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19325.1"
                     /translation="MQRRGPALLVMGLVLLLAACNSDRPEPAEADVPSAGLRLRVTRV
                     ATHPFLSRYRLTLQVEGSRGCTDTAELFPDTGYAGRRNVYAQASGAITLLGQYDARVI
                     DPAQCRIRLVEFQSVGEPSKFLGAFDVDAEKRWQFIPASMRPERPFEKH"
     gene            complement(2069..2968)
                     /locus_tag="PK589_06705"
     CDS             complement(2069..2968)
                     /locus_tag="PK589_06705"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ19326.1"
                     /translation="MAQAEFRIGIVGVGRMGANMARRLHDLTYQLVAVYDTEASRAET
                     LANELGCVAAPTPARVAELANTILTVVPDDAAMRHIYAPNTPDSLLRHATGRLFINCA
                     TLSPAAQMEVHRLVEQQGGHSLEACMASSIPQARHGTLYLMCGGRRDVFERATPLLKD
                     LSAHLRFIGPAGDAAKVKALVNMVMNANTAALAEGLGLASALGLDLTMLREVFSQTGA
                     NSRVLETDGEDMQQRAHDCYFSAAHAAKDSGIACSLAEQVGLELPLAKATYDQYRRLV
                     TLGKGELDKSAVAELTFKERLTS"
     gene            3096..3317
                     /locus_tag="PK589_06710"
     CDS             3096..3317
                     /locus_tag="PK589_06710"
                     /inference="COORDINATES: protein motif:HMM:NF023510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HNJ19327.1"
                     /translation="MQQSIIGYHQDEEGHWVADLRCGHGQHVRHQPPMTSRPWVLTEE
                     GRQVFIGTELNCKKCDEGGDGFSPAEPLS"
     gene            complement(3396..4211)
                     /locus_tag="PK589_06715"
     CDS             complement(3396..4211)
                     /locus_tag="PK589_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19328.1"
                     /translation="MQSITPHPRLRSVFTLCWMVGGAALTLGGCVSQQTYDTARHEVK
                     TRAGELAQTQADIHSLEQEREAAHLANQRDERTLANLKSELKTIQASYDQLHKANQAK
                     LALLEHNIAALRAKHQAMLKEIGDTKRIEKRLEGLTTLRDRTMGTQQSGPEAQATPVD
                     ISSPEQHMVAVITPQPSQADASSLPPIPATVPAQLPPAPAAAAAPAPATSSAAPAAVA
                     PATPNAPAKVSQAPAAAAAPPAPRPVPAAPPAEESWFSSVTGWFSSLIDWLWA"
     gene            complement(4385..5485)
                     /locus_tag="PK589_06720"
     CDS             complement(4385..5485)
                     /locus_tag="PK589_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNJ19329.1"
                     /translation="MNSDIIQRIEIWPVDIPITDPFVVATGARVTAQNLFMRVTLKDG
                     THGVGEAAPFPEVGGEDRASCLAAAQKLAAGLIGHSAGDYETLADHLTEQAPLFPAAR
                     CGLETALLDAYCRSQRLPLWQLWGKADVRERETDITIPICDLDKTLALSRGWYAQGFR
                     LFKMKVGTDVEEDIRRLQAVHEALPGISFIGDGNQGFSREDCIHFAGRVKQFGGRLVL
                     LEQPVVRDDLEGLHAIRHLTGIPVAADESVRSLDDARRVVRMQAADYINIKIMKTGVI
                     EARRIADFTRSSGLRLMVGGMLETRIAMGCSFSLVLGLGDFDVLDLDTPLLLSTDPVI
                     GGYRYQGPRLLPWQEAGLNMQMAVPTHCLTIQ"
     gene            complement(5514..6158)
                     /locus_tag="PK589_06725"
     CDS             complement(5514..6158)
                     /locus_tag="PK589_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HNJ19330.1"
                     /translation="MDQNDRLIKTKENWAKARRGGEEREVFYEGDDRLPPGQHLVETW
                     PVLDLGQKPDIPLTEWTLTIVGAVAAPMTWTWTDFLAQPQFKDISDFHCVTSWSRYDN
                     EWEGVSFKHVAAVVQPHPSARFVLFKSYDDYTTNLPLSACLDDDVLLTYKWNGRPLTK
                     DHGGPVRMIIPKRYAWKGAKWIKEITFSQHDEKGFWEVRGYSNTALPWQNDRYG"
     gene            complement(6395..6544)
                     /locus_tag="PK589_06730"
     CDS             complement(6395..6544)
                     /locus_tag="PK589_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19331.1"
                     /translation="MGPLSVHDMKGGFFLIASFVLLATICAIAALGTLMRPQRWCERE
                     TRLHV"
     gene            6981..7697
                     /locus_tag="PK589_06735"
     CDS             6981..7697
                     /locus_tag="PK589_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HNJ19332.1"
                     /translation="MTISADSSLPKARAGSGKLVLALLVGCAVAGFLYFDLGRYLSLD
                     ALKANRDQLLAFTEANYSTAVAVYVLAYCAVVGLSLPGGAIMTLAGGFLFGSVLGTLY
                     VNVGATVGATLAFLVARYLLRDWVERQFGSRLGAIQEGFSRNAFSYLLTLRLIPLFPF
                     FLVNMVSGLTRVNVGTYVAATSLGIIPGSFVFAYAGRQLGTINSLKEIVSPNVLIAFT
                     LLGLLALVPILYKRFAGHSS"
     gene            7749..9311
                     /locus_tag="PK589_06740"
     CDS             7749..9311
                     /locus_tag="PK589_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HNJ19333.1"
                     /translation="MSVRTADSQHLVLPDDEHNRKLVGNVHPSNWVNPESDGRYNIVV
                     VGAGTAGLITAVIAASVGAKVALIERHLMGGDCLNVGCVPSKGLIRAANAWAQVRDAP
                     VFGARIPDGASFDFGAAMARMRQLRARISHVDSAHRYKSLGVDVYIGQARFVGPDTVS
                     VEGAAGNRTLAFVKAAVCTGARAAAPPIPGLEEAGYLTNETIFSLTALPARVGVIGAG
                     PIGCELAQALARFGSRVSLIEAMHGILPNEDRDAAECVQQSMIRDGVALLCCGKDLRV
                     EKVSDGKRLIVDSHGRHHDLTVDEILVGVGRAPNVQGLGLEAVGVEFDKTGVTVNDRL
                     QTTNPRIYAAGDVCSRYKFTHAADAMAQILIQNALFPHPFGLGYASMASLNMPWCTFT
                     SPEIAHVGLYEAEAVKQGLQVETYTYKFDEVDRAILDGDEEGFARLHIKAGTDTILGA
                     TIVGSHAGDVISEVSVAMKAGVGAKTIAATIHPYPTRAEVIKKTVNLWRKAHFTPRTK
                     SLLTKLFAWLRR"
     gene            9318..10055
                     /locus_tag="PK589_06745"
     CDS             9318..10055
                     /locus_tag="PK589_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HNJ19334.1"
                     /translation="MRPIGPILCLALFGATVMGEAQQPALLPVGTFSAATEGSALPDG
                     WHMLTFKKIERHTQYDVIKDQAISVVRAVSEAGASGLTKAVTIDPREYPIVRWRWKIE
                     NVLNKSDVSRKEGDDYPARLYITFAYEPDRVSFGKKLKYKAGQALFGDIPIAALNYIW
                     DSKSPVGTVVDNAFTSFAKMIVVESGARRIGSWIEEERNVYQDYLSAFAEEPPAINGI
                     AIMTDTDNTKERAVAFYGDIEFVRAPK"
     gene            complement(10158..10571)
                     /locus_tag="PK589_06750"
     CDS             complement(10158..10571)
                     /locus_tag="PK589_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19335.1"
                     /translation="MKINVETSWLGIAVATLATISFAVAPASAKEKDKSGKHHPPAAT
                     TSAAAAASLLGSVPEQSAAVKPGQYDSKPGVAWKTVGGTVKDIQGETYTVEDYDGSQM
                     KVRVGQGTKQLRGHKKVGDTIRAEITHGGFANSIQ"
     gene            10898..11215
                     /locus_tag="PK589_06755"
     CDS             10898..11215
                     /locus_tag="PK589_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19336.1"
                     /translation="MKQVIWSVAILSMCVPSLVFAQAETPRIDQRQANQERRIDQGIA
                     SGQLNEREATRLNNQQEHINNIEDKAKSDGVVTKKERARIKHAQDRTSRHIARQKHDR
                     QQR"
     gene            11449..14109
                     /locus_tag="PK589_06760"
     CDS             11449..14109
                     /locus_tag="PK589_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ19337.1"
                     /translation="MDLITTHLNADFDGLASMVAARKLYPGALLVFPGGTQESVRKFL
                     ADHDLGICRLKDVALEHVHRLILVDVQEPERIGPLKVLSSRKDLDVHIFDHHGNGEGV
                     ETSAWASKTQRHIEPVGATATLLIERLQSQQVVLTAAEATVLAMGLYEETGSLAYPTT
                     TPRDLEAAAFVLRAGADLNRVAETLQHPLDPDLIALLNDLLQSGEIYYLEGRKILVAS
                     SAYDRYTGDLAEAVQRLAELQGFDAVIVAIAMDEKVQIIGRSRRPEIDVAWIAREFGG
                     GGHAVAAAASVKGRTLVEVQEQLTRLLTERYRPTLLARDVMTTPVKSIVADATVTEAE
                     RSMTTYGVNVLPVVDTKDRYIGMISRETVQKALFHQLGDQPVGDLAGSGHYSASPDTP
                     FHDIETRMIELNQRFVPVLSDGKIVGVITRTDLLRSLHDDVLASARSKPKSFIGLESP
                     GGVRKRDLKGLLRDRLPPRVHDLLHASGVLAERLGLSLYVVGGFVRDLLLGLENLDVD
                     LVVEGDGIAFARALAKERAGRVKVHERFGTAVVLLPDGFKIDVATARTEYYEYPTALP
                     TVEQSSIKKDLYRRDFTINTLAVALRPRNFGQLIDFYGGQRDLKERLIRVLHSLSFVE
                     DPTRVFRAIRFELRFDFHLSKDTLALIKGAVKMELFHRLSGQRLLDELRLLFSERTPR
                     QAVRRLADLDLLRFIHPTLTWSARLDRRLSELEAALDWYRLSCLDRTIDGWVVYAMAL
                     AETMPDEAVRDMLERFPFTEAERTAINQARFLTQPVCRTLARRAPLRPSETVAALAGF
                     SEECLVFLLAKNGSDTAKRSLSQYLTTYRDIKPALTGKALQALGLKPGPVYRTILERL
                     TEARLNGEVHTEEEERALVKDLIARRRSAS"
     gene            complement(14211..14411)
                     /locus_tag="PK589_06765"
     CDS             complement(14211..14411)
                     /locus_tag="PK589_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19338.1"
                     /translation="MDTTLTPRQITHDEKKAAEAAFAGRPFNPKWSDAARIVYDGLLA
                     AMGKEARAVDTHDELEPVEPLA"
     gene            complement(14532..15509)
                     /locus_tag="PK589_06770"
     CDS             complement(14532..15509)
                     /locus_tag="PK589_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HNJ19339.1"
                     /translation="MPTKQPLIHVLHPGLFTTVQDLGRSGYQRFGVSVSGAMDPWALM
                     VGNRLLGNPDQAAGLEITLQGPQLLFEQAVRIAITGADLSPTSNGLALPMWTVVAMPA
                     GSRLQFGMRRQGARAYLTVAGGITGPMILGSLSTHVRSGLGGLAGRAVKKWDQLFVGT
                     ASRAAAQPIGRSLPERYRPRYTGSPAVRVMPGPQSDYFTDDAIRLLTSSPYRVTTESD
                     RMGYRLEGAELPHRASAEIVSEAVCVGSLQVPSNRQPILLMADCQPTGGYAKLATMIG
                     ADRSLAAQLAPGDTLSFRMISAEKASELFRSTYAELDRILPPAVLGAKP"
     gene            complement(15499..16275)
                     /locus_tag="PK589_06775"
     CDS             complement(15499..16275)
                     /locus_tag="PK589_06775"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HNJ19340.1"
                     /translation="MTRLRHIDLNCDVGEAATPEQRDVEARLMPYVTSVNIACGVHAG
                     DAALMRSTIQLARQYDLAIGAHPGLPDRESRGRRELPLSQGFVLDLILSQVSDLMTIG
                     QAEGIRLSHVKPHGALYNMAARDPELADAIAAAIVQLNRRLILIGSAGSELLTSGRAH
                     GLAVAAEGFVDRAYQADGRLVPRTEAHAVLHDESTVLARAHALVHDGTVVAANGTMLH
                     LHIDTLCLHGDTPDALRLAHALRTMFAESGISVRRLDHAD"
     gene            complement(16272..16958)
                     /gene="pxpB"
                     /locus_tag="PK589_06780"
     CDS             complement(16272..16958)
                     /gene="pxpB"
                     /locus_tag="PK589_06780"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HNJ19341.1"
                     /translation="MIPRMIQLSESAVTVDFGDGIDLHTNELVLAFTAAVDQSKIPGF
                     REVVPTYRSATVYFDPLLTDADTLMKKVRALTVDTVVAPSRASITHTIPVWYGGAAGP
                     DLPDVARQAGLTPEEAGELHASVAYRVCMLGFSPGFPYLSTVPAPLAAPRLPTPRKYV
                     AAGSVGIAGTQTGVYPQASPGGWRIIGRTPVVLFDLHRPKPFLLAPGDQVRFVAIEEE
                     EFTQLAGDQA"
     assembly_gap    17057..17156
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17160..18431)
                     /locus_tag="PK589_06785"
     CDS             complement(17160..18431)
                     /locus_tag="PK589_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ19342.1"
                     /translation="MHGVTTYQWLVLFVAWLGWVFDAMDATIYAIVLHPALHELLQAG
                     ATGGEVTSEQIGWYGGIVFSVFLIGWALGGIGFGMAADYFGRTKTLIATILIYAVFTG
                     LAALAQEWWHLAIYRFLTALGVGGEWAAGAAIVAETWPEEKRSRAAGILQSAWAFGFF
                     IAAGCNLLLKDSGWRMLFLIGILPAFVALLVRRWVKEPERWVQAHEREPRSAHTSWLH
                     LVELFRPSLRRDTLIGSTLAFVAVFGVWGATNWAPTLIRGMPDLQGQDSAALAEKVSY
                     AIMALNAGALFGYLGFGPLAERFGRRPVFGLMCLGSLILLPATYFVPHSYAEVLLLLP
                     VLGFFNNGIFSGFPIYLPELYPTRLRATGSGFCFNAGRILASAAPFVTGWLVTMLGSF
                     NNAASTIALIYLLGLAILPFATETKGRPLPE"
     gene            18545..19183
                     /locus_tag="PK589_06790"
     CDS             18545..19183
                     /locus_tag="PK589_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNJ19343.1"
                     /translation="MPRIDYYRVLGVSRDISDDEIKKAYRKLVFEHHPDRNPNNSKAE
                     EKIREINSAYEVLGDPESRRTYDRLHWGEEMRPETVDPALILEEMEKKLFDEGRKEVF
                     AVLMKMVPRIKSELAIIRERTVAHQGYDTFKEPIVEARGAEVLDEFVTPEMEQRKHRL
                     LEVAVQMMVSQSVVARGDEGGIRALRGRLEEMFRKGRINGFATALELLYQRR"
     gene            complement(19274..20482)
                     /locus_tag="PK589_06795"
     CDS             complement(19274..20482)
                     /locus_tag="PK589_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNJ19344.1"
                     /translation="MTIAIRQIRILDGQGRKIERGTVVIEGDRIVAAGPDQTVRLPRG
                     AQRIDGRGLTILPGLIDCHVHFCLGAEADVVAAVEQESSAVTLLKAAELARRTVQAGF
                     TTVRDVGFRDHAVFALKKAIASGLTPGPRILAAGLAICMPGGHARFIGREAQGIEAVR
                     AAVQDQLTAGAEVIKVIASGGVLTPGTSPDEAQMTVEELRAAVEVAAAHGRHVAAHAH
                     GASGMKNALRAGVHSIEHATLMDEEAAGLMTQRGVYMVPTLSALATTAACPTGCGIPD
                     SARSKAKNMVRQHEKSFRAAVRRGVPIALGTDAGTPFNYHGDNAQELERMVTLGMTPM
                     DAIMTSTSAAARLLRLSAEIGTIEAGKQADLLVVEGNPLRNIGLLLKRERIAGVMQGG
                     RFVSGPLSET"
     gene            complement(20479..21081)
                     /locus_tag="PK589_06800"
     CDS             complement(20479..21081)
                     /locus_tag="PK589_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNJ19345.1"
                     /translation="MSRNGFWIVMAGVAGFVAAITYWVIALAVAESRKADMVAPERVT
                     SFIHAVIDANRANYTQNVVDKLHTQGVVEALEHWKEEKGLPLPAQFLLESGRLVAQKD
                     MKLSFRLASLTPIYVWNGPNSEFERRGLEVVMKAPEKPFTGFYQQGGVRYFQGIYADR
                     AVSESCVSCHNGHANSPRRDYKLNDIMGGVIVTIPISEAP"
     gene            complement(21078..22295)
                     /locus_tag="PK589_06805"
     CDS             complement(21078..22295)
                     /locus_tag="PK589_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNJ19346.1"
                     /translation="MSLSSHDHAFVPPHRLLMGPGPSMVHARVLHALSQPLVGHLDPI
                     FLNLMNTIQASLRTLFRTENEFTIALPGTGSAGMEAVIANLIEPGDRAIVGVNGVFGS
                     RLATMIERSGGIPLRIEAPWGQIISLDAVHDALSRGGPVKAVVLVHAETSTGAWQPLE
                     DVGALCRAHGALLIIDAVTSLGGLPVEVDAWGIDACYSGTQKCVSCPPGLAPLTVSPR
                     AMEAVRQRRTPCHSWYLDLSLIAEYWNDRSRAYHHTAPISMLYGLHEALRLIHEEGLS
                     PRFIRHQLNSDALLAGLEPLGLRPLPPAAHRLPMLSCVTLPEGIDDTLVRTQLLQDHG
                     IEIGGGLGPLRGRVWRIGLMGESCQQAHVLTLLNALEDIFAQHGWLDQPGRAVQAAVE
                     TYTQSQLSARRGA"
     gene            22508..24001
                     /locus_tag="PK589_06810"
     CDS             22508..24001
                     /locus_tag="PK589_06810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNJ19347.1"
                     /translation="MDADESTGPVSQSSRPPAPCVGAPPPPHAYNPGFSRAVTLLSAV
                     VLSASIAVVAWLSFDIPRVERVADPGRALSHMVGRLMDQADGVKTLPVWEQFLYDLTM
                     GSDANDREQAIEWYRELAEESSDPFVDLHLAILEGEAGHLPEVRQRISRWIRQSAPYP
                     LFARLLQAGYVDAHVARSAGFDLQASLAEQPVSGWFYSRLAARIAERAGDRPLLVTIE
                     TADQQRIETLVWRSRAFALLELGLMVVGGMVLMVWVRRGTGANMFRVGSAELPPLWAG
                     RLGAGVLLRGGAVGALLTVAFLFAAGDVPSLRVVAVPLSNLPLLALAYYHLLRPQRQT
                     FWRGLGLSLEPRHVGQLSLAVLAVVAAGLVGEWVLGRLAEPLNLISHWTEWFDADLVW
                     GTPPTLIVSLMEYVVFAPVFEELAFRGLLFGVFRRRFQWGTAAMLSAALFAIAHGYGL
                     IGFLSVFWSGVIWAWAYERTGSLWPGMIGHAVNNLLVCLSVMALLRP"
     gene            complement(24088..24696)
                     /locus_tag="PK589_06815"
     CDS             complement(24088..24696)
                     /locus_tag="PK589_06815"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNJ19348.1"
                     /translation="MTATKKTKPLKRASPPATKAEVHVGDIVRRLRKSHHLSVRTLAD
                     KCGFSPSFISQVELRQASPSIASTEKIASALGVTLGEFFRAIDPTPPAIIRSDSRPVV
                     QSEWSRAKIEALGPFNKDSQFEPMVITIQPGGASGHKPYVRQAEQLAVVLMGSLELTL
                     EEDVHQLKRGDAAGIPAGNRHCWRNISKRPAQILIVTAHRRL"
     gene            24984..26330
                     /locus_tag="PK589_06820"
     CDS             24984..26330
                     /locus_tag="PK589_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNJ19349.1"
                     /translation="MTHPDAMWGGSLKDKATWLTLTIFVSACALLWLTGVTSTKHLVD
                     NLIAGYLLSWGLYAFLSDLPRREIQGRFILMTLCGIVVLGTAELLGVTGLVNYQMLFG
                     TQGREWFDRSGYVRDPELGYRRQAHYQEEGAFVRGNIGEALCLPANAPQRFDLRYDHR
                     GFRNDTDLDRAEVVVIGDSYVESPMLPGDSLLTTQLSHRLRTSVANLGISGYGPEQEL
                     VVLKRYALDLQPKTIVWVFFEGNDLYQLDPEEQNLSAVPDTRAEHDEYWMRSLTRNLL
                     LLSRKAVQGCVPHPDYLRLRGTFHDTEGNETLLYFWERLGELKPTDRLRLERLRSILT
                     EAYHVSRQQGIRLVVAFAPVKHRVHQGLKNFEPLTAEMREWPLNELPDEVASMLNTLS
                     PDIEFIDLTPPLREAAAQGVLTYLPDDTHWTAEGQRVAGEAIHHLLAGGQTRVAKQ"
     gene            complement(26338..27240)
                     /locus_tag="PK589_06825"
     CDS             complement(26338..27240)
                     /locus_tag="PK589_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19350.1"
                     /translation="MRLCPETRTRIGSNRQQQTNRQFGYDARMPSLRPIVILGTGYTG
                     RWIQKLAREQSLPILASSRQPEHHLSDIAQTARIRFDLESPSTWSALPLDADLIWTFP
                     AVPLEQVKAFATHSCSHSRRLVVLGSTSAYDRGDEPLTDMPPWIDETCPVNVDLPRVQ
                     GEEYLRTKHGGIILRVAGIYGPHRNPVEWIRQGRVGPTNKFVNLIHVEDLAALCLLAL
                     QIGRAGDIYNISDGLPRRWTEICVEVSTRWGIVSPREATSSQPGKRINNRKALEGLRY
                     TLRHPDLYRTLATLQQESGVRNCP"
     gene            27297..28400
                     /locus_tag="PK589_06830"
     CDS             27297..28400
                     /locus_tag="PK589_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ19351.1"
                     /translation="MIGRPTDAQAQRRQRILAGLAGLLACLFFVLDLQLPLGVANAVL
                     YSAVVLLSSASQYRWLPIATATACSLLTIIAIPFSPRIPNLPPWFEWVNHLFSLFGVW
                     APVVFVYQRRRTERLLEEINERLERGVETRTADLAASRLALERSEEQLHTLTGRILTA
                     QEEERRRIAQDLHDDVNQRLALLMLELQSVDRQIGVSPVAAQDGIHNVLKGLEALSDD
                     VRYMAYRFHPSILDDLGLKAALQRLLDDFSTRTGVKALFVHQPLDHPLDKTASTALYR
                     VVQESLSNIARHAKATRVEVEVTVEEEGVVVVVRDDGKGFDQLTVERCEGGLGLLNMR
                     ERLLSVQGTCEVESSPGKGTTVSMYVPLVRVAI"
     gene            28397..29041
                     /locus_tag="PK589_06835"
     CDS             28397..29041
                     /locus_tag="PK589_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ19352.1"
                     /translation="MTLPRVLLADDHTLVLEGFRRLLDDQCELVGTVGDGRSLLEAVP
                     QLKPDIVILDISMPVLNGIDAARVMKVKFPQVKVVFITMHADPAYVRAAFEAGASAYL
                     LKRCVGEELAQAIRAVRSGNFYVTPLVTKEVVESMLRGVDGGAPSGPELTTRQREVLQ
                     LLAEGHTVKDIAGTLKISPRTVEFHKGQIMEQLNLHTTVELVKYALAQGLTSQT"
     gene            29193..29624
                     /locus_tag="PK589_06840"
     CDS             29193..29624
                     /locus_tag="PK589_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ19353.1"
                     /translation="MRKKISRPRVLLGDSSRSMLAFLEILLESHFDVVGSETEGDALV
                     LTAKTLAPELVILDFSLSFLDGLSVGRQLQEAVPDSKVVFFSSHEDAAYVTAAFEVGA
                     MGYLVKHRTVDPGHYLRRVLDGERVCCPDDLHKRVGRPKKK"
     gene            30060..34511
                     /locus_tag="PK589_06845"
     CDS             30060..34511
                     /locus_tag="PK589_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNJ19354.1"
                     /translation="MTPVLPFHPIIADWFTAKFAVATDVQQQAWPEIQSGRDLLISAP
                     TGSGKTLAAFLSCIDRLFQQALRCDLRDETQILYVSPLKALSNDVQKNLQQPLLEIGQ
                     AALSAGLLLPDLRVIVRTGDTPMSERQQMLKRPPHILITTPESLFILLTAEKSRAMLK
                     TVRTVIVDEVHAVAPNKRGAHLALSLERVAALAGGAVQRIGLSATQRPIERMGHFLVG
                     NRSSSPQTQDEHSAAGCSGDEMPVANHVAIIDVGHRRDMDLAVEVPKDELGPVATNAI
                     WSDIYDRVAALVEAHRSTLVFVNTRRLAERVSHYLEDRLRHLGEGVVAAHHGSLSREI
                     RLSAEDRLKTGAVRVVVATASLELGIDVGTVDLVCQIGSPRSIATCLQRVGRAGHWVH
                     AIPKGRLFATTRDELIECAALIRSIRAGVLDHIEVPPAPLDVLAQQIVAASATQAWDE
                     TELYKLCCRAMPFRDLTRETFDAVIAMLADGFATSRGRGRAYVHHDRINHQIRGRRGA
                     RLAAITSGGAIPDTANYAVIAEPDGTVVGSVDEDFAVESLAGDIILLGNTSWRIKGVE
                     TGKMRVEDAQGAPPTIPFWRGEAPARTADLSAEVARLRADLDHRLDPNVPAPPPSAPP
                     VQWLKQECGLDQRGAEQAVQYILAGKSVLGTVPTQHTIVAERFFDESGGMQLVIHAPF
                     GGRVNRAWGLALRKRFCVTFDFELQAAATDEGIVLSLGEKHSFPLETVFAFLNVKTLR
                     DVLTQAVLQAPMFMTRWRWNASRALALLRFAGGKRVPPQIQRMRAEDLLAAVFPDAIA
                     CQDNFQGERTERQIPDHPLAQETIRDCLTEAMDIDGLAAVLNRIESGAIACVAVDTPM
                     PSAFCHEILNANPYAYLDDAPLEERRARAVDMRRTLPAALAGEMGALDQSAIDQVREE
                     SWPDVRDAEELHDALLTLGWVPCACMPGWDLLVPRLEVAGRVATLWRGDNKQGWVAAE
                     NHQYGGLLFPDTRIDPESAPTHEPLLEREEVLQRIVLGWMESVGPVTAADLAARLGLT
                     VPDVDGAMLRLEAQGHVLRGRFSTQVSHTTDGVVEWCHRRLLARIHRLTVGRLRKEIE
                     PVTAAEFMRFLFQWQHASPGARLHGEAGLLEVAKQLGGFEAAASAWEAQILRVRLAKY
                     QPEWLDRLCLGGALMWGRITPHPRLMQELALLPGRRVVPTRVAPVSLFAREDAPVLLE
                     ATGEDLARLDLSSKLSVSAQAIRHCLQQRGASFFSELLHGARLLASEVEDGLWELVAA
                     GLVTADGFDNLRALIDPRRRRAEASDRNRRPRHVGGRWSLLRPAVGQSQTGPQAATTL
                     RASSSSAHVVGSSERAQATERIARQLLQRYGVVFRDLLARESIVSSWRDLLVCYRRLE
                     STGELRGGRFVSGFTGEQFALPEALEALRALKKRPGTATQQEIKISAADPLNLAGIIL
                     PGPRIAAVPSNFVVFREGVVIRTVTGRSATDRQEPPILEVAQRDLRS"
     gene            complement(34512..35366)
                     /locus_tag="PK589_06850"
     CDS             complement(34512..35366)
                     /locus_tag="PK589_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ19355.1"
                     /translation="MNAPRNPLSIHRIFHPTDFSQDSQVAFVHALKLALVFRAELTIM
                     HVDPDVSPEGFEDFPRIRPTLAQWGLLPESSSKSDVTTLGIRIRKVRALAADAKQAIM
                     HHLTATPTDLMVLATHKHEGFAGWIHHAVAEPVSREMHVTTLFVPSNVAGFVSRTTGE
                     TSLHRVLLPISSHPPSQPAIDAATVLVSQLSKPPVTMTLIHAGAEADVETLALPQKAD
                     WSWNQLFGKGDPVEWILAAGAEFDVDVIVMATKGQDSLLDMLRGSTTERVLRGARCPL
                     LAIPAPLI"
     gene            complement(35429..35824)
                     /locus_tag="PK589_06855"
     CDS             complement(35429..35824)
                     /locus_tag="PK589_06855"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HNJ19356.1"
                     /translation="MTGHRRSLIAAASGLSLLALLSACSFKATFKETTDTTSNITGTT
                     SGRIWWNEDGLLKSEHKVAAFTAYNADNLEADAARGHGEYLASLDALAHLPDAAVFQP
                     TAQAAFSRWSQAGSSSATELVKALQTAHR"
     gene            36077..36517
                     /locus_tag="PK589_06860"
     CDS             36077..36517
                     /locus_tag="PK589_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19357.1"
                     /translation="MKVGMGLCVIAGILCAAPLVRAETVPLKPGEYQVTAVTESSSGE
                     AGKPDTHTRCVKEEHLANPDAVFNYYALNGFKPNPANKVMNVSMHGGTVSYDIEGLYA
                     MTRVEGTVSTTGFSVVRKATPKGDKALSVTMKVDGKRTGDCATK"
     assembly_gap    36752..36851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<36852..38686)
                     /locus_tag="PK589_06865"
     CDS             complement(<36852..38686)
                     /locus_tag="PK589_06865"
                     /inference="COORDINATES: protein motif:HMM:NF021597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HNJ19358.1"
                     /translation="MEQQHANPEPDPSASRQDPRYYQIASLGTLLIYGLCWLHFDLSI
                     RQIVVTIGTALLTQYVGTILVNLPAFDAKSALISSLSLCLLLRTDDLAVAALAAFLAI
                     GSKFVLRWHDKHLFNPTNLALTVMLGSGLGWISPGQWGQTAWLAFLIACLGSLVVTRA
                     ARADVTLAFLACYVGLLFARAVRLGDPLTIPLHQIESGTLLIFAFFMISDPKTTPDSR
                     IGRMVYALLVSFTALYVQFGLFKPNGPLWGLIACAPLVPVIDHVLPGSRYQWAHASTG
                     GSPHPAPIPVRTLSTVLSSTKEVSMRRAIIPSVLFTLSLLAWGGDALAFCGFYVGKAD
                     TKLFNKASEVAIARHDDKTVITMANDFKGEVKEFALVVPVPTVLEKEQIHVGDPALLK
                     HLADYSAPRLVEYFDQNPCLRYELMERRSMDAMKSVAPSAAPRERDKALGVTVEAQYT
                     VGEYDILILSGKESSGLEIWLTENGYRIPQGASAVLQSYLKQGMKFFVAKVNLGEQAK
                     LGLTYLRPLQIAFESPKFMLPIRLGTVNADGAQELFLYFLTKQGRVETTNYRTVRLPE
                     AQEVPLFVKEKFGDFYRDLFSQQVKRESERGIFMEYAWDMNWCDP"
     gene            38832..39464
                     /locus_tag="PK589_06870"
     CDS             38832..39464
                     /locus_tag="PK589_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19359.1"
                     /translation="MPIQVIVLIGFLSLLPGCSLEQELSRTPRTAVEQVLLTQAVNQA
                     LTNLTVQLPVGVNVELDATGLESDRSRLRMTNSDLGSLNSPNRDILYIRDTVAAELGR
                     RGYLVRARDTESPYLVRVMAESFGTMQGITFFGMPPVQSVLIPFALPELTLYKQQNQS
                     GYARLHLDIYDNRTGEFLGSSATLVGRTFYNQYTMLFFLTWNRTDIDAPP"
     gene            39513..39875
                     /locus_tag="PK589_06875"
     CDS             39513..39875
                     /locus_tag="PK589_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19360.1"
                     /translation="MLELLLVLASLVVIGLTGLLTVVATPPRMVELGLWALALGLLIG
                     IPTGWWYHVVLYRALSARMALPRRWWQRPVELHPLLQPDEFRRVRPWFVAGALGFVLC
                     FTGGVAAIAGMLVMRFYP"
     gene            complement(39900..41207)
                     /locus_tag="PK589_06880"
     CDS             complement(39900..41207)
                     /locus_tag="PK589_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19361.1"
                     /translation="MADHELEHLLGGFAANTLTPEERKQLYAAALHDQQLFTALADEQ
                     ALKELLANPDVRRRLLQALQHTSPTATGGTDTWWGWLKKPAGLAWAGGLAAAVFAIAF
                     GTRIYQESLDRARQSIVTEEVKPAAPPMPRVQPSMPAPQSAATPDPQAMEPADAAKEN
                     RRTDTRAKREQPLAGRASEQSAAKSSTDTAPPRRERDESSNNAPMAAAEKKSTGTPPS
                     ADRTLAAPAPPSTAPQPSAAPPLLQAPAGFSAAGAPAPKKSARALFYGEQSSPSLGQA
                     KPLGLRYSFVTRGTDGRTQEITAAMVARSSDPVRLTVEATQESYVQLLQNLGSAGTRL
                     WWPPQETGKISLKLQPGKRTDIPIPPPAENGLVTLIIRLSSKPFGPLTMQEVGMLDRF
                     SASLLIEAVSPGTTAGVQEQATYVVSQDPSTSAQLAVEIPVVQ"
     gene            complement(41256..41753)
                     /locus_tag="PK589_06885"
     CDS             complement(41256..41753)
                     /locus_tag="PK589_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ19362.1"
                     /translation="MTRDEILTHLRERILTFATSRLQKDVAEDLTQEVLMLLHEKYVR
                     VTELTELVPLAFQVLRFKMLDAHRKSLRRGEYNDHSVDDLPSADPGTDPASQLDQKQR
                     VDRLMAALGQLGDRCRQLFKWKLEGNSFPEIQTLMGQASINTIYTWDLRCRKQLLSLM
                     GGTWE"
     gene            complement(41765..42757)
                     /locus_tag="PK589_06890"
     CDS             complement(41765..42757)
                     /locus_tag="PK589_06890"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNJ19363.1"
                     /translation="MTRPVTKTLIDCHVHLAALPDGDNGCYISPKTLRSPLFRFLLWK
                     HGLSPKHPREANQKYLADLLTELRASRHVHKAVLLGMDGAYDQNGRLNQANTDFLIGN
                     DYVLKTAQAHPDELLAGVSINPQRRDAIEEVHRCADAGAVLVKVLANAQQFDPANKTY
                     TPFYRALAERKLPFLSHVGYEFSLIGKDQSVGEPDRLRVALDEGVTVIAAHACSYGLI
                     LYEKFLPTLHDLVQRYPHFYADISALTLPNRLRMLLHLRNYPQVHERLLFGTDYPLPV
                     FHLAAWGRVAMGRLRQLILTKNRFDRQVDVCQGLQVGFRSIGDLIPHASGQTPH"
     gene            complement(42820..>46040)
                     /locus_tag="PK589_06895"
     CDS             complement(42820..>46040)
                     /locus_tag="PK589_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HNJ19364.1"
                     /translation="LTSFVAGFFADRLSKRTVIITMKAVEVLLMAGATLALLSHPTGG
                     AWALVMLACMGVHSALFSPAKYGILPELLPHAQLARGNSILELFTFLAILTGTTAGGF
                     LLAGAGNQPWLAPLALTILALIGFAAARAVPPVPAARAAGGLFDTLRGALSALRADRL
                     LRLAITGAVFFWTIASLVVQNVLVYAKAVLGLSDALATVPSALISVGIGLGALVVGRL
                     SRSRVEYGLVPLGAAGVATFLLTLGWWVPAFTGTLVVMIALGMSSALIFIPINALVQW
                     RAPHDRRGSVIALENICVFTGILLGSLSGGVLANVGLSTVGIFLATAGLTTIGTAWAM
                     WLMPETFLRLVLVLLTHTIYRLRIVGQAHVPVTGGALLVPNHISFIDGFLLIASVDRP
                     IRFVVDAHYVNLPFFKPFMRALNVIPIASDGGPRVILRALRDAGQALDHGDLVCIFPE
                     GQITRTGNLLPFRRGFERIVKGREVPVVPVHLDRVWGSIFSFVGGRFVTKWPERLPYP
                     VTVSFGAPVPAGTPAHELRRLVRELGETAWHWRKSSRRPLHAAFVRAMRRHPFRLAMA
                     DATKPHVSSLQALIGAIALARALTPHWQGQPSVGLLLPPTVAGALTTVAAALAGRSSV
                     NLNYTVGKAGLESAVRQAQLRTIVTSRKFIEKAKLEVPDGPTILWLEDIGATIGSLQK
                     MLAAALALFAPLRLIESSCGQTRRVTMDDLATIIFSSGSTGEPKGVMLTHFSIDANVQ
                     AVSQVLPLDPEDRILGILPLFHSFGYLVFWYVTLNGAASVFHPSPLDVTAVGELCAKY
                     RLTFLVCTPTFLQLYQRRCTPEQFSTLRVVLTGAEKLPLRLCQSFQDRFGIGPIEGYG
                     VTECAPVIAVNCPDFRAAGFFQPASRRGTVGQPLPGVSVRIVDPETDAILPPGMPGML
                     LVKGPNVMNGYLGREDLTAQAMRDGWYITGDIATLDDDGFLTITDRLSRFSKIGGEMV
                     PHRLVEDALQQASGEELQVCAVTGVPDERKGEQLAVLHTLAEERIPHILAKAQAAGLP
                     NLFLPSHSRCFKVEALPILGTGKLDLRALKRMAMERLNISGT"
     assembly_gap    46041..46140
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(46502..48385)
                     /locus_tag="PK589_06900"
     CDS             complement(46502..48385)
                     /locus_tag="PK589_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HNJ19365.1"
                     /translation="MNPVTSAELKPSREQHLVHALRRAVHLETNGRRVSARFTTARLT
                     IFAATLLGSIIPHKLGWTLFGNLVLASGFVLFLIVAWYHNRLEDRLHRLRRWQGIKRV
                     HLARLSLQWAEIPLREVTVPERHGYARDLDLTGPRSLLHLIDHSISSQGRQRLASWFL
                     DQPPLAAPWSSRQALIRELTPLSLLRDRLVLEAQAVEEADIDGRRLLAVLQKRVDDSG
                     LMPMLLTESALAVTTAILLLADLISGLGNYWMLSFGLYAFLFLSVRSRSEEIFDHAQS
                     LHLQLERLSAVLQYLERRSYRSTPHLAKLCAPLMQQDSSPSRSLKQAASLMNRLSVRA
                     HPLVHYLINAVGPWDLFHTYRLQQLQDRIASVAPLWFELLADLDAAASLATFAHLHPN
                     YLWPTLHHAGADGQENGNAPELAAEGLAHPLIPAAVRVANQVTLQGRGRIFLVTGSNM
                     SGKSTFLRTIGINTCLAQAGAPVCATAFRWSLVRIGSCIRVDDSLDGGLSFFYAEVKR
                     LKTILDSTRDRQGPPVLWLIDEVFKGTNNRERLIGGKALITALAGGNGFGLVTTHDLE
                     LAELEKQLPTVTNVHFQETVVAGALHFDYRLKPGPCPTTNALRIMALEGLPVESADPM
                     PPR"
     gene            48415..49350
                     /locus_tag="PK589_06905"
     CDS             48415..49350
                     /locus_tag="PK589_06905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNJ19366.1"
                     /translation="MTSTSKVDQSSSSDSAAVQTGDWIQVDVRTSLDAGELLGMLNDP
                     DVTGAWQEGDEVHLYWPAGRCGPDTWQGLKRALTQLGHPDPDHAVTINHLANQDWNAQ
                     WSRLVEPIKIGRVLIRPSWRTVSLNSDEIELIIDPKQAFGTGHHATTQLLIEWLQQAV
                     RPDDRVLDVGTGSGILAMVALRLGATEALGVDFDAVAVDCAREYAQVNGFDTRLTLAV
                     GQAQAPQAHDAFDPTLVLANLDRQTLLTSADSLCAYAVRGARLFLSGLLAEQRSEIEA
                     AYATRGVYMKAVRERDGWLALEATAMETCEGGLCS"
     gene            49358..49849
                     /locus_tag="PK589_06910"
     CDS             49358..49849
                     /locus_tag="PK589_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNJ19367.1"
                     /translation="MTRSATPYVHQISVSDGGVPKLAVPSARITIDGLAGDRQRNRKY
                     HGGKDRAVCLFSLERIEALRAEGHTIGPGSSGENLTVAGLDWQHMAPGDQLTIGEHVR
                     LELTSYTTPCRLNAPWFKDEDFRRIAQEVHPGWSRLYARVLNEGVVKPGDVVVVDACP
                     GER"
     gene            49877..50539
                     /locus_tag="PK589_06915"
     CDS             49877..50539
                     /locus_tag="PK589_06915"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ19368.1"
                     /translation="MMKRVLEPELMDDVAQALAYAKADFAEENQSFVDRFREYFPAWD
                     GGHVVDLGCGPGDIPIRFLRAYPHARVTAVDASRPMLDLAADAIAGAGLTDHITLRCE
                     RFQSLVLPEQADALLSNSLLHHVPNPLQFWFRLKQLAKPGACILVMDLLRPDSPEAAQ
                     ALVEQYAADEDPILKRDFYHSLLAAFTEDDVAAQLAEMNLSRLLIDVPDDRHWVVGGI
                     IP"
     gene            50779..51474
                     /locus_tag="PK589_06920"
     CDS             50779..51474
                     /locus_tag="PK589_06920"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNJ19369.1"
                     /translation="MIPEGVFRMGADGTDALEDEKPQHDVWLSRFEIDRHEVTTGHYA
                     EFLASTKRPAPWQWESLDLSQHGDRPVIGVSWFDADAYCRWRGTRLPTEAEWEKAARG
                     TDGRLFPWGNHSPQEETANFGLGARFSYSQVLAPVERYEQGRSPFGLYQMAGNAGEWV
                     ADWYGAHYYENGPRQNPSGPDAGAFRVVRGGSWSDLPKYLLTYGRFKLPPETRNSYTG
                     FRCARDGGVLASP"
     gene            complement(51517..>52779)
                     /locus_tag="PK589_06925"
     CDS             complement(51517..>52779)
                     /locus_tag="PK589_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNJ19370.1"
                     /translation="PAIIPTPSPVAVPPPPAPTPVVAPVVTGTAITSSPAALALSAVK
                     GQTAVGTLSLRKGGTDQHSYYLSTNQGWVWMNPPYGSTQTITTETDQLVITAQTANLA
                     AGTYSAVVYVVQSGPNNFQNMLRIPVTLTVTASAVAPPPPPPAAVPPAIVPTPKPVVL
                     APPPPPAPAVVPPPPAPAPPAPAPVTVATGPIQVTPSSLSLTSANNVGTLTLRKTGTD
                     QHAYYLSTNQGWIWMNPPYGSTQTITTESDQLVITAQTAGLAAGTYSGVVYIVESGPN
                     NFANTLRIPVTFTVAAGQTASSTPTTPTQPTVASPPPPPPSPPSPVPVTTTPTPTSSA
                     SSTAPKTASATVSWNANTESDLAGYRIYVGTKSGSYGVVGPFEVTNGTSFTVPNLPLG
                     ATYFFAVSAFDKSGNESAKSAEVSKSLF"
     assembly_gap    52780..52879
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<52880..53265)
                     /locus_tag="PK589_06930"
     CDS             complement(<52880..53265)
                     /locus_tag="PK589_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19371.1"
                     /translation="MKTPKSVLVIGVAGCLSSALCSTALPPTAAAADTTLSLLQQSST
                     DAVAIMAQNSGTAPIFTSPTALVLSAQKGQTAVGTLNLKKSSTDQHSYYLSTNQGWVW
                     MNPPYGSTQTITTETDQLVITAQTANL"
     gene            complement(53526..54365)
                     /locus_tag="PK589_06935"
     CDS             complement(53526..54365)
                     /locus_tag="PK589_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNJ19372.1"
                     /translation="MKERRNNRHHFTTMEEALSFHDQAGHRIAAVLATPPQSTDSVAV
                     LCHGFLSHKNSTSNQALAELLKTRGIATLRFDCFGHGESDGPFAKLTTSIGVTQARAA
                     LTYAMTRGYRRLALVGSSFGGLVSLLAASAWTDLHNSAPADVPPLACLALKCPVVDFG
                     EELRLELGEDGLQEWQRTNTIPDLHGGPSRLPLDYAFYQDCLNRIAYEPARAITTPTL
                     IVQGDHDEYVPLHQSQRLYASLAGPKHLEILPGADHRFTNPSDFQLMLTLLTDWIAAP
                     PAA"
     gene            complement(54346..55635)
                     /locus_tag="PK589_06940"
     CDS             complement(54346..55635)
                     /locus_tag="PK589_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19373.1"
                     /translation="MSRGLGSPLSFLLAGWGWVLLTALLGLAAFGGIVRGGPLPSGLR
                     LLHVHGVLIGGVLQLMTGLAIAAHDLSGHEGKRTTHRLLLLSMNLTAVGFAAGAWFRN
                     PTLTLAAGFLLAALFVPMARHMLTALRTCSGWTPLVHVYFGISFAGLFGSMVLGGLLA
                     DAWYPTWHGILRLGHLHAGLLLFLILGAVGTIQLTLPMMVHRPLHSPGMGQAVMLLLP
                     ASAAGLITGFVLSSVHIQLAAGTVLLIALALCCINLLRTWSQAGQPGSAATDHLLTAL
                     SFLLIMTFIGLAFGINVLSTPPAMPYGTLHLVAYTHTAFLGFLLQSMVGGLSFTLPSL
                     LATSRIANQKKRGTYREDLERIMNRWRAAQVATVSFGSLGLVLVASLTWNLPLSSPWL
                     QTSTWISLGLLLFHFTMVTVKLAQATGAQPSHEGAAK"
     gene            complement(55641..>56390)
                     /gene="thiD"
                     /locus_tag="PK589_06945"
     CDS             complement(55641..>56390)
                     /gene="thiD"
                     /locus_tag="PK589_06945"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNJ19374.1"
                     /translation="GAGIQADLKAMSANGVYAMSVITAITAQNTEEVADVFELPTSII
                     AAQLDAIFDDFDVAAVKTGMLSSAEIIRTVVKLLKPQQVKHLVVDPVMIAKGGQALLK
                     PDAIDTLKSALFPLALLVTPNVHEAQQLSGIEITSLADARRAAKVIHQFGCANVLIKG
                     GHLQKERGTDLLYDGRFFNIFKGEYIDTPHTHGTGCTFASAIAAHLARGKSVSDAVQT
                     AKTYLTEAIRHGLAIGHGKGPTNHFYFLNQG"
BASE COUNT        10985 a        15707 c        17858 g        11440 t
ORIGIN      
        1 tcatggtgca tgtatggccg ttctcgattg tcgggaggct gttttatcgc ctacggtggt
       61 ccaagccctg ctatcagatc atcaatcgac aagcccgccg ggcgtttgac tcaatcccgc
      121 agccgctaca cccgctgagc gtcgaagcgg tccaggcact caacacggat ggcatctatc
      181 gcagctcggt cgacgcgctc gtgcacgatg ccaacctgtt ccacgagctg tcacacgatg
      241 cgcagatgct tttggcggga caggacgttc aacgcaagat ccggcagcga tacaatccgc
      301 atgatgccaa atggtatgtg gtgcgcgcca tcggccaccg cgccaagcga ccgtcgttgc
      361 cctcatcctt cgtccggttt tttctccacc cgcaagtcct cagcatcgcc agtacgtatc
      421 ttgggctgca ggttcgtctg aactataccg atgtctggca taacatcccc gtccgcgagg
      481 gcgaaccgcc catctccgcc gaattctggc atcgcgatca tgaggataag cgggtcatca
      541 agctgtttgt gtttctgacc gatgtcgacg agaccatggg tccgttcacc tacgtaagac
      601 gatcgcaggt ggggggcgag ttcggccatg tctatcccgc catccctccc attggtcgtt
      661 atcccgagca ggatgccctg agtcaactga tcaccggcac cccactgccg tcggtgtcct
      721 gtgtcgggcc ggccggaagt atcattttgt gtgataccag tggcctccat cgcggtggcc
      781 gatcggtgac gaaaccgaga atcgtcctgg tcggagtgta cacgtccaac gccgggatgg
      841 atgccgcacg ctatgccctt cctcgctctc tcccccacga acacttgtcg ccggccgcaa
      901 agttcgccct ctattcgtaa cccgtcacgc ctctgtgtcg aatccgacta cgggaacccg
      961 agtattgaca gagcggtgtg atggccgtag gctcgcgcgg tgcagtgtgg atgattcatg
     1021 acggacgaag accgaatgaa ggaggcatca gatgggagag cgaatacagg tgtccgggcg
     1081 ggtggtgaga tgtgtcggcc tccttgctgc ggcgttcgtc gccgccggat ggctgggagc
     1141 ggcgcaggcg gcggatgaat tgccgaaaga gtcggtgttg ccggcagccc tcgccggaaa
     1201 ggccgtgcag gcggccctcg acgcctgtaa aaaggatggc tatcgggtca gcgcatcagt
     1261 cgtagatcgt gccggtgtgc ttcgggccat gctgcgggct gatggtgccg gtccccatac
     1321 cgtcgacagc agccgaaaga aggcctacac ggcagccagt ttgcgccgtg cgaccagtga
     1381 gttggcggac atgatcgcca agcagccggc gctgcaggcg ttgcgagaaa tgaacgaaag
     1441 cattttgatg gtgggaggcg gtttgcccat tgaaattgcc ggtgaagtgg tcggcgcgat
     1501 cggggtcggc ggcgcgccgg gaacgcacct ggatgatgcc tgtgccgaag ccggtcttga
     1561 tgccatcggc gcagcctcga agatgcctgc cgcgaaatga acctggtgca gcgacgagga
     1621 ccggccttgt tggtgatggg gctggtcctc ctcctggcag cttgtaacag tgatcgtccg
     1681 gaaccggctg aggcggatgt gccctcggca gggttgcgct tgcgggtgac acgcgttgcc
     1741 acacatccct ttttgtcccg ctaccgttta acgctgcagg tagaaggatc tcgcggctgt
     1801 accgatacgg cggagttatt tcctgatacg ggatatgccg gcaggcgcaa tgtgtatgcg
     1861 caggcgtcgg gggcgattac tctgctgggg cagtatgatg cccgagtgat tgaccccgcg
     1921 cagtgcagaa ttcgactggt ggagtttcag tcagtagggg agccatcgaa gtttttaggc
     1981 gcgttcgatg tggatgctga gaaacgttgg caatttatcc ctgcctcaat gaggcccgag
     2041 cgcccgttcg aaaagcacta agtgatcctc aagaagtcag ccgctctttg aaggtcagtt
     2101 ctgcgactgc cgacttatcc aattcaccct ttccgagcgt caccagccgg cggtattgat
     2161 cataggtggc cttcgcaagc ggaagttcca gcccgacttg ttcggccaaa ctgcaggcaa
     2221 tacccgaatc tttcgcagca tgggcggcgg aaaagtaaca atcatgcgcg cgctgctgca
     2281 tgtcttcgcc atccgtttcc agaacgcggg agttggcgcc ggtctgagaa aagacttctc
     2341 gcagcatcgt cagatctaat cccaaggcgg acgctaatcc gaggccttcc gccagggcgg
     2401 cggtattggc gttcatgacc atgttcacca gcgctttcac cttggcggcg tccccggcgg
     2461 gcccgataaa gcgcaagtgc gcgctcagat ccttgagtaa cggggttgcg cgttcgaaca
     2521 cgtcccggcg cccgccgcac atgagataga gcgtgccatg gcgggcctgg ggaatactgc
     2581 tcgccatgca ggcttcgagg ctgtggccgc cctgttgttc caccagcctg tgcacttcca
     2641 tctgggcagc gggagagagc gttgcgcaat tgataaagag acggccagtc gcatgccgta
     2701 acaaactgtc cggcgtattt ggcgcataga tgtggcgcat ggctgcatca tccgggacca
     2761 ctgtcaggat cgtgttggcc agttcggcga ccctggcggg agttggagcg gccacacagc
     2821 cgagctcgtt ggcgagtgtt tcggcgcgtg atgcctcggt atcgtagacg gcgacaagct
     2881 gataggtgag gtcgtggagg cgtcgggcca tgttggctcc catgcgtcct accccgacga
     2941 tgccgatacg aaactccgcc tgtgccatcc gcaacctcct ggaaaacatg gtactctgac
     3001 tggtgtggca gtgtaccaca gtctattcag gccccggtat cgcggcatcg gctgtgaggc
     3061 ccgggggcgc tcggataacg acgagggagt gtgtgatgca acaatcgatt atcggatatc
     3121 accaggatga ggaggggcat tgggtggccg acctgcgctg cgggcacggc cagcacgtgc
     3181 gtcaccagcc gccgatgacg agtcgtccgt gggtactgac cgaagagggg cggcaggtat
     3241 ttatcggcac tgaattaaat tgcaagaagt gcgatgaggg gggagatggg ttctcgccgg
     3301 cagagccgct ttcgtaagcg ccgtggagag aaagcgatac ctgtggcccg gaccatcaca
     3361 aggtggtgag gtccgagccg attcgcatat ctatcttagg cccagagcca atcgatcaaa
     3421 gaagaaaacc agccggtcac gctggaaaac caggattcct ccgcgggggg tgctgcaggg
     3481 accggccgcg gggcaggcgg agcagcggca gcagccgggg cctggctgac ctttgccggt
     3541 gcattcggcg tagcgggtgc aacagccgct ggtgcggctg aggaagtcgc cggtgctgga
     3601 gccgcggctg ccgctggagc agggggaagt tgggccggga cggtcgccgg gatcggcggc
     3661 agtgaggatg cgtcagcctg cgagggctgc ggtgtgatca ccgcgaccat gtgctgctcc
     3721 ggtgaactga tatcgaccgg cgttgcctgt gcttccggcc cggattgctg cgtgcccatc
     3781 gtccgatcgc gcaacgtggt caggccttcc agccgtttct caatccgctt ggtatcgccg
     3841 atctccttca gcatggcctg atgcttggca cgtaaggcgg caatattgtg ctcaagcagt
     3901 gccagtttgg cttgattggc cttgtgaagt tgatcgtagg aagcttgaat ggttttcagc
     3961 tcgctcttca ggttggcaag cgttcgctca tcgcgctgat tagcgaggtg tgctgcctct
     4021 cgttcctgtt ccaggctgtg aatatcggcc tgagtctgcg ccagctcgcc cgcccgcgtt
     4081 tttacttcat gcctggcagt gtcgtaggtc tgctgcgaga cgcagcctcc cagcgtcaat
     4141 gccgcgcctc cgaccatcca gcagagcgtg aagacagatc tgagccgcgg gtgtggggtg
     4201 attgattgca tgggacgatc ctcccggata cgaacggtgg cgataggtgc aaaaggtggt
     4261 cccctcagcg gagccacggt gcctgaggtt aatttttcag aaaagtgaaa aaacggcaaa
     4321 atatttcggg aaacctgcag gcagcggtag ggagagtctg agcgacggcg aattgagcgt
     4381 ccggttattg aatagtgaga cagtgggtgg gaacggccat ctgcatgttg agtcccgcct
     4441 cctgccaggg gagaaggcgg gggccctggt agcgataccc gccgatgacg ggatcggtcg
     4501 aaagcaggag cggtgtgtcg agatccagca catcgaaatc gcccagcccc aacaccaggc
     4561 tgaacgaaca gcccattgcg atgcgcgttt cgagcatgcc gccgaccatg agtcgaaggc
     4621 cggaggagcg ggtgaagtct gcgatgcgcc gtgcttcgat tacccccgtt ttcatgatct
     4681 tgatattgat atagtcggcg gcctgcatcc tgaccacgcg gcgcgcatcg tccagtgatc
     4741 ggacggattc gtcagctgcg acgggaatgc ccgtgagatg ccgaatggcg tgaagtcctt
     4801 cgaggtcgtc acgaaccacc ggttgttcca aaaggaccag gcgccctccg aactgtttca
     4861 ctcggccggc gaaatggatg cagtcctcgc gggaaaagcc ctgattgcca tcaccgataa
     4921 agctgattcc cggaagcgcc tcatgcacag cctgtaggcg gcgtatgtct tcctcgacgt
     4981 cggttccgac cttcatcttg aacaggcgga agccttgcgc ataccagccg cgcgagaggg
     5041 ccagcgtttt gtcgaggtcg cagatcggaa tggtgatgtc ggtttcacgc tcccggacgt
     5101 cggctttccc ccacagttgc cacagcggga ggcgctgaga ccggcaatag gcgtcgagaa
     5161 gggccgtttc caggccgcat cgagcggcgg gaaagagcgg agcctgttca gtcaggtggt
     5221 ctgcaagggt ttcgtagtct ccggcagagt gtccgatcag tccagcggct aatttctggg
     5281 cggcagcaag gcaggaggct cgatcctcgc ctcccacttc aggaaacggc gcggcctctc
     5341 cgacgccgtg agtcccgtcc ttcaaggtca cgcgcatgaa gaggttttgc gccgtcacac
     5401 gcgcgcctgt ggcgacgaca aagggatcgg tgatcggaat atcgaccggc cagatctcga
     5461 tgcgctgtat gatgtcggaa ttcaccaggg gcgtcgtggc tgcaggtgga ggctcatccg
     5521 tagcgatcgt tttgccaggg aagggcggta ttggagtagc cgcgcacttc ccagaaaccc
     5581 ttctcgtcgt gctgcgagaa ggtaatttcc ttgatccatt tggcgccttt ccaggcgtag
     5641 cgcttcggga taatcatgcg caccggacca ccgtggtcct tcgtgagcgg ccgaccgttc
     5701 catttatagg tgagcagcac gtcatcgtcc agacaggcgc tcagcggtaa attggtcgtg
     5761 tagtcgtcat aggacttgaa cagcacgaag cgggcggagg gatgcggctg gaccacggcg
     5821 gccacatgct tgaagctcac cccttcccat tcattgtcat accggctcca ggatgtcacg
     5881 caatgaaagt ccgagatatc tttgaactgc ggttgcgcca gaaagtctgt ccaggtccag
     5941 gtcatcgggg ccgcgactgc gcctacgatg gtgagcgtcc attcggtaag cggaatatcg
     6001 ggcttctggc caaggtcgag caccggccat gtctcaacga ggtgctggcc tggcgggagc
     6061 cggtcgtcgc cttcataaaa cacctcacgc tcttctcccc ctcgccgggc tttggcccag
     6121 ttctccttgg ttttgatcaa tcgatcattc tgatccattc cggcctccct gttcacagag
     6181 taggacaaga tgccctgttc ttacaagggt tgaagcgagc aatgacaaag ggcgaggaca
     6241 catgcggaag gcatgaaagg gcccatgatt ctgcgccaat gtgcggttta gaaggattct
     6301 caatgctgga gcaggagata cattgcgcac agagagggaa ggccgcaggg ggcgtcgacg
     6361 tcaggcagcc tcaaacgggg gccgactggg aaccttagac gtgcagtctg gtctcgcgtt
     6421 cacaccagcg ttgcgggcgc atcaacgtgc ccagtgccgc aatggcgcaa atcgtggcca
     6481 gtaacacaaa gctggcgatg aggaaaaatc cacccttcat atcgtgcacc gagagcggtc
     6541 ccatgacgtc acccccttca tatgccctaa ttgtaccact tcactccgtg cgccagccgc
     6601 agccacaacg ccatgattct tcaggtgaat tttttctgag acggcaatgg caatggggac
     6661 aggggcgcat ccacacgtgt aaagcggttt cgtaagcggg gtgcgaggta cgggtctgct
     6721 gtggaaccga atgtgttgtc ggcatggcag cgtccaggac atcaatgatc gcgtacacgg
     6781 acgacattac ctccactcgc ctcccggctt caagagttct ttcggaggtt gccgccctgc
     6841 acttgagggt accgatgaac gctgggagca aaaaaatgca gaagcgagga aaaagaggcc
     6901 gcagcatgga cgacgaggac gtgatctgat tgacacagga gcaggtagac acgatgatac
     6961 cgccgggagt accgaacacg atgacgattt cagccgattc ttctcttccc aaggcgcgcg
     7021 ccggtagcgg caaacttgtg ttggcgttac tcgtgggctg tgccgtggcg ggatttcttt
     7081 atttcgatct ggggcgctac ctttccctgg atgcgttgaa ggccaatcgc gatcagctgc
     7141 ttgcattcac ggaagccaac tattcgacgg ccgttgcggt gtatgtattg gcctattgcg
     7201 cggtggtcgg gctgtcattg ccgggtggcg cgattatgac cctcgccgga ggatttctct
     7261 tcgggagcgt gctgggaacg ctgtacgtga atgtcggcgc cacggtcggc gccaccctgg
     7321 catttctggt tgcgcggtat ttgctgcgag actgggtgga gcggcaattc ggtagccgac
     7381 tcggagctat ccaggagggg ttctctcgga atgcgttcag ctacctgctg actctgcgat
     7441 tgatcccgct ctttccgttt tttttagtca acatggtgtc gggattgacc cgggtgaacg
     7501 tcggcaccta tgtggcggcg acatccctcg gtattattcc gggaagtttt gtgtttgcct
     7561 atgcggggcg ccaactgggc accatcaatt cgctcaagga aatcgtgtcc cccaatgtat
     7621 tgatcgcgtt tacgctgtta ggattactcg cgctggtccc catcctctac aagcggtttg
     7681 ccggtcactc gagctgaaca gagagagacg cctctggcga gggccttgcg tagagaaagg
     7741 tcgtgtagat gtctgtaagg actgccgata gtcagcacct cgttctgccg gatgacgagc
     7801 acaaccgcaa actggtgggc aatgtgcatc catccaattg ggtgaatcct gaatcggacg
     7861 gtcgatataa catcgtcgtc gttggggcgg gaacggcagg attaattacg gcagtgattg
     7921 ccgcgagcgt cggcgcgaag gtggcgctta tcgagcggca cttgatgggt ggcgattgtc
     7981 tcaatgtcgg ctgcgtgccc tccaagggat tgattcgcgc cgcaaacgcc tgggcacagg
     8041 tgcgcgatgc gccggtattc ggcgcccgca ttccggatgg ggcgagcttc gatttcggcg
     8101 cggcaatggc cagaatgagg cagctgcgag ctagaatcag ccatgtggat tctgcacacc
     8161 ggtataaatc gctgggcgtg gatgtctata tcggccaggc ccggtttgtc ggcccagata
     8221 cggtgagcgt ggaaggcgcg gcaggcaacc ggactttagc cttcgtcaag gcggctgtgt
     8281 gtacaggggc aagggccgca gctcctccca tccccggcct ggaagaggcg ggctatctca
     8341 ccaatgaaac gattttttcg ctcacggcgc tccccgcgcg cgtcggcgtc atcggggccg
     8401 gcccgatcgg gtgcgaattg gcgcaggctt tggcacgatt cggcagccga gtatcgctga
     8461 ttgaggcgat gcacggcatt ctcccgaatg aggatcgaga cgcggcggaa tgcgtccaac
     8521 agtcgatgat ccgtgacggt gtggccctgc tctgttgcgg gaaggatctc cgggtggaga
     8581 aggtctcgga cggcaagcgg cttatcgtcg attctcatgg acgacaccat gacctgacgg
     8641 tcgatgaaat cctggtaggg gtaggccgtg cgcccaacgt acaaggcttg ggattagagg
     8701 ccgtcggcgt tgaattcgat aagaccggcg tcacagtgaa cgatcgcctg cagacgacga
     8761 acccgcgtat ctacgctgcc ggggatgtct gctcgcgcta taagttcacc catgccgccg
     8821 atgcgatggc gcagatcctc attcaaaacg cgctctttcc ccatcccttc gggttgggat
     8881 atgcgagcat ggcgtcgctc aacatgccct ggtgcacctt cacctctccg gaaatcgccc
     8941 atgtcggttt gtacgaagcg gaggcagtga aacagggact gcaggtcgaa acctacacct
     9001 ataagtttga tgaggtcgat cgagccattc tggatggtga tgaggagggt tttgcccgtc
     9061 ttcatatcaa agcgggtacc gatacgattc tcggggccac aatcgtcggg tcgcacgcag
     9121 gggacgtgat cagtgaagtc tctgtggcga tgaaagccgg cgtcggcgcc aagacgattg
     9181 ccgcaaccat ccatccctat ccgacacgtg cggaagtcat caagaaaacg gtcaatcttt
     9241 ggcgcaaggc gcatttcacc ccgcgcacca agtcactgct gaccaagtta tttgcctggc
     9301 tgaggcgatg aatacctatg cggcccatcg gcccaatcct ctgtctcgca ttattcggcg
     9361 caacggtgat gggggaggcg cagcagccgg ccctgttgcc cgtgggaacg ttttccgccg
     9421 caacagaggg atcagcattg ccggatggct ggcacatgct gactttcaag aaaatcgagc
     9481 ggcatacgca atatgacgtc atcaaagacc aggccatatc tgttgtcaga gcggtgagcg
     9541 aggctggcgc ttccggactg accaaagccg tgacgatcga cccgcgtgaa tatccgattg
     9601 tccgctggcg ttggaagatc gagaatgtgt tgaacaagag cgacgtgagc cggaaagaag
     9661 gcgacgacta tccggcgcgg ttgtacatta ccttcgcgta tgagccggat agggtgagtt
     9721 tcggaaaaaa actcaaatac aaggccggcc aggcgctatt cggcgacatt cccatcgccg
     9781 cgctcaacta catctgggac agcaagagcc cggtgggaac cgtggtggac aatgcgttca
     9841 cgagttttgc caagatgatt gtcgtggaga gtggggctcg acggatcggt tcctggatcg
     9901 aagaggagcg gaacgtctat caggattatc tctcggcgtt cgcggaggaa ccgcccgcga
     9961 tcaacggcat tgcgatcatg acggatacgg ataatacgaa agaacgcgcc gtggcctttt
    10021 acggtgatat cgagtttgtt cgcgctccga aatagctgac gtagcaacgg atgtgaggga
    10081 tgaaggagag gccgacctgt gcagggtcgg cctcttcgga caccggcgag ctgaggcggg
    10141 ttcgcaatgt cagcgggtta ctgaatggaa ttggcgaagc cgccgtgagt aatctcagcc
    10201 cgaatggtgt cgcccacctt tttgtgacct cgaagttgtt tggtgccttg ccccacacgg
    10261 accttcattt gactgccgtc gtagtcctcg accgtatagg tttctccctg aatatctttg
    10321 acggtaccgc ccacggtttt ccaggccacg cccggtttcg agtcgtattg gccaggcttg
    10381 acggcagccg attgctcagg cacggatcct aaaagagacg ccgcggcagc tgcggatgtg
    10441 gtcgcggccg gcgggtgatg tttgcccgac ttgtcttttt cttttgctga tgccggtgca
    10501 accgcgaagc tgatggtggc gagggtcgcc acggcgatgc ccagccatga agtttcaacg
    10561 ttaatcttca taagaacctc cttcagtgat gaggactgcg ggtcggcgat tgactgagca
    10621 agccccatgc ctccgagatc cgggggtgat tcgctcggat ttgtgccggt tgtgtccggt
    10681 tggcgtgata agacaggacc ggcaggagag gaaaacgcca acatctgttc aattttgccc
    10741 agccattctc cggatccttc ctgacttctg ctgtttcgct gggacacgca gttctccgta
    10801 tagcgttctc ggaaatcaaa aactcgttgt caacgaacca gccgccttgt acgttgaaat
    10861 gaacgacagg gctttcgcga taaccaaggg aggatgtatg aagcaggtga tttggagtgt
    10921 ggctatactg tcgatgtgtg tcccgtcatt ggtcttcgcg caggcggaga ccccaagaat
    10981 cgatcagcga caggcgaatc aggagcgacg gattgatcaa ggcattgcca gcggtcaact
    11041 caatgaacgt gaggccactc gcttgaacaa tcagcaggaa cacattaaca acatcgagga
    11101 caaggccaag tccgacgggg tggtgacgaa gaaagaacgc gccaggatca agcatgccca
    11161 ggatcgtacg tcgcgccaca tcgctcgcca gaagcacgac cgccagcagc gttaatgttt
    11221 ccatccggac cgacttcgtc agaaactcag catgtcggtc cggggcactt ggtcggtctc
    11281 tgcgctcgtg cttgttgtcg ggtgccttcc atgccgtcca caggtcatac tgtgggcggc
    11341 atggttgtat ctgacctcaa ttgtcatagc cgttccagtc ggcacccggt ccctggattt
    11401 cacacatcgc gcctgaatcg attcacttga tcctgctaga atgccggcat ggatctcatc
    11461 accactcacc tgaatgcaga ctttgacgga ttggcctcca tggtggcggc caggaaattg
    11521 tatcccggtg ctcttctggt ctttcctggg ggcactcagg aatccgtccg aaagtttcta
    11581 gccgatcacg acctgggcat ctgccgcctc aaggatgtgg cattggagca tgtgcatcga
    11641 ttgatcctgg tcgatgttca agagccggag cgcatcggtc ccttgaaggt gctgagttcg
    11701 cgcaaggacc tggacgtcca tatatttgac catcatggaa acggcgaagg ggtagagacg
    11761 tcggcctggg cgtcaaagac acagcgccat atcgagccgg tgggagccac tgcaacgctg
    11821 ctgatcgagc gcctgcaatc gcagcaggtc gtcctcactg ctgctgaagc caccgtgctg
    11881 gccatgggcc tgtatgagga aacaggctcg ctggcctatc ccacgacgac tccgcgcgat
    11941 ctggaagccg cggcgtttgt cctccgtgcc ggcgccgatt tgaaccgggt cgcagagacg
    12001 ctgcagcatc cgcttgatcc ggatctcatt gccctgctga acgatctgct tcaatcaggg
    12061 gagatctact acttagaagg ccgaaaaatc ctcgtcgctt cgagtgcata cgatcgatat
    12121 acgggagacc ttgccgaggc ggttcagcgg ctcgcggaac tgcaaggatt tgatgcggtc
    12181 atcgtggcca tcgcgatgga cgagaaggtt cagattatcg gacggagccg caggccggag
    12241 atcgatgtgg cgtggattgc gcgcgagttt ggcggcggcg gacatgccgt ggcggccgct
    12301 gccagtgtta aaggccgtac gctcgtggaa gtgcaggaac aactgactcg tctgctgacc
    12361 gaacggtacc gcccgacgtt gttagcccgg gatgtgatga ccacgcccgt caagtcgatt
    12421 gtcgcggatg ccaccgtgac ggaggcggaa cggtccatga ccacctacgg tgtcaacgtg
    12481 ctgccggtgg ttgatacgaa agatcgatac atcggcatga tttcacggga aaccgttcag
    12541 aaggccctgt ttcatcaact cggagatcag ccggtaggag atctggccgg cagcggacac
    12601 tatagcgcat cgcccgacac gccgttccat gacatcgaga cacggatgat cgaattgaat
    12661 cagcgtttcg taccggtgtt gtccgacggc aagatagtgg gtgtcatcac gagaaccgac
    12721 ctgctccgta gcttgcatga cgatgtgctg gcatctgcgc gcagcaaacc gaagtcattc
    12781 ataggactgg agtcacccgg aggagtgcgg aagcgggatc tgaaaggact gctgcgggat
    12841 cggttgcctc ctcgcgtcca tgacttgctg cacgcatcag gtgtattggc cgaacgactg
    12901 ggcttgtcac tctacgtggt cggaggattc gtccgcgatc tgttgctcgg tctcgagaat
    12961 ctcgatgtgg acctggtcgt cgagggggat ggcatcgcgt ttgcgcgcgc cctcgcgaag
    13021 gagcgtgccg gtcgcgtcaa ggtgcatgaa cggtttggca cggccgtggt actgctgccg
    13081 gacgggttta aaatcgatgt cgcaacagcc cgtaccgagt actacgagta tccgacggcc
    13141 ttgccgactg tggagcaaag ttccatcaag aaggatcttt accggcggga cttcaccatc
    13201 aatacgttgg cggtggcgct gaggcctcga aatttcggtc agctcatcga tttttacggc
    13261 gggcaacgcg atctgaaaga gcggctgatt cgtgtgcttc atagtttgag ttttgttgaa
    13321 gatccgacgc gggtgtttcg ggcgatccgg ttcgaactgc gcttcgactt tcatttgagt
    13381 aaagacacgt tggccttgat taagggcgcg gtgaagatgg aactgttcca tcgactgtcc
    13441 ggccagcgcc tcttggatga actgcggttg ttgttttctg aacgcacccc gaggcaggcg
    13501 gtgcgccggc tagccgacct tgatctgttg cggtttattc acccaacgtt gacctggtct
    13561 gccaggctgg atcgacggct cagcgagctg gaagcggcgt tggattggta tcggctgtcg
    13621 tgtctcgacc ggaccatcga tggatgggtg gtctacgcca tggcgctcgc ggagaccatg
    13681 ccggatgaag ccgtccgcga catgctcgaa cgttttccct ttaccgaagc ggaacggacc
    13741 gccataaacc aggctcggtt cctgacgcag ccggtatgcc ggacgttagc caggcgtgca
    13801 cctctccggc catcggaaac ggtcgccgcc ttagccggct tctcagaaga gtgcctggtg
    13861 ttccttctgg cgaagaatgg ctcggacacc gctaaacggt ctctgtccca gtatctcact
    13921 acgtatcgtg acataaaacc ggctctcacc ggaaaggcgc tccaggccct tggtctgaag
    13981 ccaggccctg tctaccggac catattggag cggttgaccg aggcgcgttt aaatggagag
    14041 gtacacactg aagaggagga acgggcactg gtcaaggatc ttatcgcgcg acggcgttca
    14101 gcctcctagg ctgcagcgcg aggtggcgat gccacgcatc ggcgatggcc acaaggtgag
    14161 agaggggagg cctatgcgca cccctaggcg accgaactgc agcgagggga ttaagccagc
    14221 ggctcaaccg gctcgagttc gtcgtgtgta tcgacggcac gagcctcttt ccccatcgcg
    14281 gcgagaaggc cgtcgtagac gatgcgagcc gcgtcagacc atttgggatt gaatgggcga
    14341 ccagcgaaag cggcctcagc cgcttttttc tcatcgtggg taatttgtcg aggagtcagt
    14401 gtggtgtcca tacgaataat aatagatcgc ttcagggcaa tgtcaaggac tctgcgcctc
    14461 gaagaacccc tctcgttcgt tgggggctgt gcagaaatct ccggcagtga tcaacgcaga
    14521 gacttcacta ttcacggttt tgccccaagc acggcagggg gaaggattcg atcaagttcc
    14581 gcatacgttg accgaaacag ttcagatgcc ttctctgcgc tgatcatcct aaaggacagg
    14641 gtgtcgcccg gtgccagttg cgcagcaagc gaccgatcgg caccgatcat cgtggcgagc
    14701 ttggcatacc cgcccgtcgg ctgacaatct gccatcagga gaatcggttg tcgattggac
    14761 ggaacctgaa gactgccgac gcataccgct tcggagacaa tctccgctga agcacgatgc
    14821 gggagctccg cgccctccaa gcgatagccc atccggtcgg attccgtcgt cacccggtat
    14881 ggactgctgg ttaacagtcg aatggcgtcg tcggtaaaat aatctgattg cgggcccggc
    14941 atgactcgca cggcgggaga gcccgtgtac cgcggtcgat atcgttccgg cagcgaacga
    15001 cctatgggct gtgctgcagc tcgtgaagca gttcccacga agagttgatc ccatttcttc
    15061 accgctcggc ctgccagccc gcccaatcca ctccgcacat gggttgaaag acttcccagt
    15121 atcattgggc cggtgatgcc gccagccact gtgagatagg cgcgggcacc ctgtcgtcgc
    15181 atcccgaact gtaaccggct tccagccggc atggcgacca cggtccacat gggaagcgcg
    15241 agaccgttgc tggtgggtga aagatcggca ccggtgatgg cgatcctgac cgcttgctca
    15301 aacagcagtt gaggcccctg gagtgtgatc tccaaaccgg cggcctggtc aggattaccg
    15361 agcaggcgat tgccgaccat gagcgcccac gggtccatcg ctccactcac cgacacgccg
    15421 aatcgttgat atccggaacg accgagatcc tgcacggtgg tgaagaggcc gggatgcagc
    15481 acatgaatga gcggctgttt agtcggcatg atcgagtcgt ctgacggaaa ttccgctctc
    15541 cgcgaacatt gttcgcagcg catgggccag ccgaagagca tccggggtat cgccgtggag
    15601 gcagagggta tcgatgtgga ggtggagcat ggtcccattg gctgcaacaa cagtcccatc
    15661 gtgtaccagt gcgtgcgccc gcgcgagcac agtcgattca tcatggagca ccgcatgcgc
    15721 ctccgtcctg ggtactaaac gaccatcggc ttggtaggct cgatcgacaa atccttccgc
    15781 ggcgactgct agcccatgcg cccttccaga ggtgaggagt tcggaaccgg ccgatccgat
    15841 cagaatcaac cggcgattga gttggacgat tgcggctgcg atcgcatccg caagttccgg
    15901 atcacgtgcc gccatgttgt agagggctcc gtgcggtttc acgtgagaaa gccggatgcc
    15961 ttccgcctga ccgatcgtca tcaaatcact cacctgcgag agaatgaggt cgagcacgaa
    16021 cccttgggag agcggcagct cccggcgtcc gcgtgattcc cgatcaggta acccgggatg
    16081 cgcaccgatc gccaggtcgt actgtctcgc cagttgaatg gtactgcgca tcagtgccgc
    16141 atcgcctgca tgcacgccgc acgcgatgtt caccgaggtc acgtacggca tgagacgagc
    16201 ctccacatcc cgttgctcag gcgttgccgc ttcgccgacg tcacagttca gatcgatgtg
    16261 gcgcaaccgg gtcatgcctg gtctcctgcc agttgcgtga attcttcctc ctcgatcgcc
    16321 acgaatcgca cctggtcgcc cggagcgagc agaaacggct tgggcctgtg gagatcgaat
    16381 agcacgaccg gcgtgcgccc gatgatgcgc cacccaccgg gactggcttg gggatacacg
    16441 ccggtctgtg tgcccgcgat gcccactgag cctgcggcca catatttgcg cggcgtcgga
    16501 aggcgcggcg cggcaagcgg cgcgggcaca gtgctcaggt agggaaagcc gggactgaat
    16561 cccagcatac acacacggta cgccacggac gcatggagct cgccggcttc ttctggagtg
    16621 agaccggctt gccgcgctac atcgggcaga tccggtccgg ctgcgcctcc ataccaaaca
    16681 ggaatggtgt gagtaatgga cgcgcgcgaa ggcgcgacta ctgtgtccac cgtcaatgca
    16741 cgcacctttt tcataagtgt gtcggcatca gtgagtagcg gatcgaaata gactgtggct
    16801 gaccggtagg tgggaaccac ttctctgaag cccggaattt tcgactggtc caccgcagcg
    16861 gtaaaggcca acaccagttc attcgtatgg aggtcaatcc cgtctccgaa gtcaaccgta
    16921 accgcggact cgctgagctg gatcatccga ggaatcatag tcatcgcctg cggcgcgaga
    16981 tgctcacaca ctcacgaggg aagttcattc cggtaacggt ctccccttgg tttcggtcgc
    17041 gaatggaagt atggccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagtt
    17161 cattccggta acggtctccc cttggtttcg gtcgcgaatg gaagtatggc caagccgagc
    17221 aggtagatca acgcgatggt acttgcggca ttgttgaacg atcccagcat ggtgacgagc
    17281 cagcccgtga cgaagggggc cgcggaggcg agaatgcgtc cggcattgaa gcaaaagccg
    17341 gagccggtgg cacggagccg cgtcggatac agctcgggca gataaatcgg aaagccgcta
    17401 aagatgccgt tattgaaaaa tcccagtacc ggcaacagga gcagcacttc tgcgtagctg
    17461 tgcggcacga aataggtcgc cggcagcagg atcagactgc caaggcacat caatccgaac
    17521 acggggcgac gtccgaaccg ttccgcgagc gggccgaatc ccagatagcc gaaaagcgcg
    17581 cctgcgttca gggccatgat cgcgtaactc actttctcgg cgagcgcggc ggagtcctgg
    17641 ccctggagat cgggcatccc acggatgagg gtcggcgccc agttggtggc gccccagact
    17701 ccgaagaccg cgacgaaggc taatgtagac ccgatcagcg tatcgcgacg taaagagggg
    17761 cggaacaatt cgacgagatg gagccagctc gtgtgggctg atcgcggttc tcgctcatgg
    17821 gcctgcaccc agcgctcggg ttctttcacc catcgtcgga cgaggagggc cacaaaggcc
    17881 ggaaggattc cgatgagaaa gagcatccgc cagccggaat ctttcaacag caggttgcag
    17941 ccggcggcga taaaaaagcc gaaggcccag gctgactgta agatgcccgc cgccctggat
    18001 cgcttctctt caggccaggt ctcggccacg atggcggctc ccgccgccca ttctccgccg
    18061 acgccaagcg ccgtcagaaa ccgatagatc gcgagatgcc accattcctg cgcgagtgcg
    18121 gccaagcccg taaacacggc atagatcaga attgtcgcga tcagcgtttt ggtgcggccg
    18181 aaatagtcgg cggccatacc gaagccgata cctccgaggg cccacccgat gaggaacacg
    18241 gaaaagacga tgccgccgta ccatccgatt tgttcggacg tgacctcgcc gccggtggcg
    18301 ccggcttgca agagttcatg cagcgcggga tggagcacga ttgcatagat cgtggcatcc
    18361 atggcatcga atacccagcc gagccaggcg acgaacaaca ccagccattg atatgtcgtg
    18421 acgccatgca tccaagccac gctgcggttc tcctctgtgc gggtcgtgcg cgccacgcta
    18481 cggggagaac gcggcgctgt caaggtgaag cgcgcaaagc ggacatggta tagtccctcg
    18541 cactatgccg cgtatcgatt attaccgcgt cctcggggtg tcacgagaca tttctgatga
    18601 cgagatcaag aaggcctacc ggaagctcgt cttcgagcat catcccgatc gcaatcccaa
    18661 caacagcaag gctgaggaga agatccgtga gatcaactcc gcctatgaag ttctcgggga
    18721 tcccgagagc cggcgcacgt acgatcgcct gcattggggc gaggagatgc ggccggagac
    18781 cgtcgatccc gcgctgattc tcgaggagat ggagaagaag ctcttcgacg agggccgcaa
    18841 ggaagtgttc gccgtcctga tgaaaatggt gccgcggatc aaatccgaat tggccatcat
    18901 tcgggaacgc acggtggcgc accaggggta tgatacgttc aaggaaccga tcgtcgaggc
    18961 gcggggggct gaggtgctgg atgaattcgt gacgccggag atggagcaac gcaagcatcg
    19021 cctgctcgaa gtggccgtac agatgatggt gtcgcagtcc gtggttgcca ggggcgacga
    19081 aggcgggatt cgcgcgctac gaggccgtct ggaggagatg ttccggaagg gacgaatcaa
    19141 tgggttcgcc acggcgctgg agctgctgta tcaacgcaga tgaataagaa ggtaacgctc
    19201 acgcgtgaag agtacgccac acgcgtacga tgaaacggtt ccggagcgtt gcttcctcgg
    19261 acgcggtttc cctttacgtc tccgaaagcg gtcctgatac aaatcgcccg ccttgcatca
    19321 ctcctgcgat ccgttcccgt ttcaggagca gtccgatatt tcttaacgga ttgccttcca
    19381 ccaccaagag atctgcctgc ttgccggctt caatggtgcc gatttctgcc gaaagtcgca
    19441 gcaatcgcgc ggcggcggac gtgctcgtca tgatggcatc catcggtgtc atgcccaggg
    19501 tgaccatgcg ttccaattcc tgcgcattgt cgccgtggta attaaaaggt gtgccggcat
    19561 cggttcctag agcaatgggg acgccacgcc ggacggcggc gcggaaactc ttctcgtgtt
    19621 gccggaccat gtttttggcc tttgagcggg cgctgtcggg gatgccgcag ccggtcggac
    19681 aggcagccgt ggtggccaga gcggatagtg tgggcaccat atacaccccg cgctgagtca
    19741 tcaggccggc ggcctcctcg tccatgagcg tggcatgttc gatcgaatgg accccggcgc
    19801 gcagggcgtt cttcatcccc gaggcaccat gggcatgagc cgccacgtgc cggccatggg
    19861 cggcggcgac ttcaacggcg gctctcagtt cttccacagt catctgggcc tcgtccgggg
    19921 aggtccctgg agttaggacc ccgcctgagg cgattacctt gatcacttcc gcgccggccg
    19981 taagctgatc ctgcacggcg gcccgcacgg cctcgatccc ctgtgcttca cggccaatga
    20041 agcgggcatg tccgccgggc atacagatcg caagtccggc ggcgaggatg cgtggccccg
    20101 gcgtgagtcc cgaggcgatg gccttcttta aggcaaacac ggcatggtcg cggaagccga
    20161 catccctcac ggtggtgaag ccggcctgca cggtgcgccg cgccagttcc gcggccttca
    20221 gcaacgtcac cgccgaggat tcctgctcga cggccgcaac gacatcggct tctgcaccca
    20281 ggcaaaagtg gacatggcaa tcgatgagac ccggaagaat cgtcaggcca cggccgtcga
    20341 tgcgttgtgc ccctcgaggc agacgtacgg tctgatccgg tccggcagcc acaatgcgat
    20401 cgccttcaat cacgaccgtg ccgcgctcaa tcttgcggcc ttggccgtcg aggatacgaa
    20461 tctgtcggat ggcgatggtc atggggcctc gctgatggga atggtgacga tgacaccgcc
    20521 catgatgtcg ttgagtttat agtcgcgcct ggggctgttc gcatgcccgt tgtgacagga
    20581 tacgcagctc tcggagaccg cccggtcggc gtagatgcct tggaagtacc gcaccccgcc
    20641 ctgctgataa aagcccgtga acggtttctc cggcgccttc atgacgactt ccaggccgcg
    20701 tcgttcgaac tcactgttcg ggccgttcca cacgtaaatc ggggtgaggc tggcgagccg
    20761 gaaactcagt ttcatgtctt tctgggcaac cagccggcct gattcgagaa ggaattgggc
    20821 gggcagggga agtccttttt cttctttcca atgttcgagg gcttcgacca cgccttgggt
    20881 gtgcaacttg tcgacgacgt tctgcgtgta gttggcgcga ttggcatcga tcaccgcatg
    20941 aatgaaactg gtcacccgtt cgggcgcgac catgtcggct ttgcgtgact ctgccacggc
    21001 gagggcgatg acccagtagg tgatcgccgc cacgaagccg gctacccccg ccatgacgat
    21061 ccagaacccg tttcgactca tgcacccctc ctcgccgaca actgtgattg ggtataggtt
    21121 tcgaccgccg cctgcaccgc gcgacctggc tgatccagcc agccgtgttg cgcgaaaatg
    21181 tcttccaagg cgttgagcag cgtcagcacg tgcgcctgct gacaggactc gcccatcagc
    21241 ccgattcgcc agacccgccc gcgcagcggc cccaggccac ccccgatttc aatcccgtgg
    21301 tcctgcaaca gctgcgttcg caccagcgta tcgtcgatgc cctcgggcag ggtgacacag
    21361 ctgagcatgg ggagtcgatg cgccgccgga gggagcggcc gtaggcccag aggctcaagg
    21421 ccggcgagta aggcgtcgct gttgagttga tggcggatga accgcggtga cagtccttcc
    21481 tcatggatca gccgcagggc ttcgtgcaat ccatacagca tagagatggg cgccgtgtgg
    21541 tgataggcgc ggctgcggtc attccaatat tcggcaatca atgagaggtc aaggtaccaa
    21601 ctgtgacagg gtgtccgtcg ctgtcgaacg gcctccatgg ctcgggggct caccgtcaac
    21661 ggggccaggc caggcggaca gctcacgcat ttttgcgtgc cgctgtagca ggcatcaatg
    21721 ccccatgcgt caacctcgac ggggaggcct ccgagggatg tgacggcatc gatgatcaat
    21781 aaggcgccgt gcgcccgaca aagggcgccg acgtcttcga gcggttgcca ggcgccggtt
    21841 gaggtttccg catgcaccag taccaccgcc ttgaccgggc cgcctcgaga caaggcatcg
    21901 tgcacggcat ccaacgaaat gatctgtccc cagggcgcct cgatacgtag cgggataccg
    21961 ccgctccgtt caatcatggt ggccagtcgt gaaccgaaca cgccgttgac ccctacgatg
    22021 gcgcggtcgc ccggctcgat gagattggcg atcacggcct ccatgcctgc cgatcccgtg
    22081 cccgggagcg cgatggtgaa ctcgttctcc gtccggaaca gggtgcggag cgaggcttga
    22141 atggtattca tcagattcag gaagatcgga tccagatggc cgaccagtgg ctgcgacagg
    22201 gcatgaagca cgcgcgcatg caccatgctc gggccggggc ccatcagtaa tcgatgcggg
    22261 gggacgaatg cgtgatcgtg cgaggaaagg ctcatggttg acgctgatcg tgtccgctcg
    22321 ccacggaagg tcgcaccgtc tcgggagcaa cgcctggcgc agcgagtggc gtagtatagc
    22381 gaatccagag ggggttcagc catgagattg tggcgagatc ccggcaccct ctgttgcggg
    22441 atgctctgca gtttcaccgc cataaggccg gtggtatact ccggcgcgcc ttacccttgg
    22501 aggtctcgtg gacgctgacg aatcgactgg tccggtctcg caatcatctc gccctccagc
    22561 gccctgtgtc ggggcgcctc ctccgccgca tgcgtacaat cccgggtttt cccgtgcggt
    22621 cacgttgtta tctgccgtgg tgctgtcggc ttcgatcgcg gttgtggcct ggttatcgtt
    22681 cgatatcccg agagtggagc gggtcgcgga tcccggccgg gcgctgagtc atatggtcgg
    22741 tcgtctgatg gatcaggcag acggcgtgaa gacgctgccg gtgtgggaac agttcctcta
    22801 tgacctcacg atgggaagcg atgcgaacga tcgtgagcag gccatcgagt ggtatcgcga
    22861 actggccgaa gaatcgtccg atccgtttgt cgatttgcac ctggccatcc tggagggcga
    22921 ggcggggcat cttccggagg ttcggcaacg tatctcgcga tggatcaggc agtccgcacc
    22981 gtatccgttg tttgcgcgac tgttacaggc tggatacgtc gatgcgcatg tggcgcgcag
    23041 cgcggggttc gatctgcaag cctctttggc agagcaaccc gtttccggct ggttctacag
    23101 ccggctggcg gctcgtattg ccgaacgcgc aggggatcgt ccgctgctcg tcacgattga
    23161 gacagccgac cagcaacgca tcgaaacatt ggtctggcgc tcacgggcgt tcgccctgct
    23221 cgagttgggc ctcatggtcg tcgggggaat ggtcctgatg gtatgggtgc ggcgaggtac
    23281 aggcgcgaac atgtttcggg tcggatccgc cgagttgccg cccctgtggg caggccgtct
    23341 gggggccggt gtgttgctgc gcgggggtgc cgttggtgcg ttgttgacgg tggcgtttct
    23401 gtttgccgcc ggcgacgtgc cctccttgcg ggtcgttgcg gtgccgctgt cgaacctgcc
    23461 gctcctggca ttggcctatt atcacttgct tcggccccag cggcagacat tctggcgagg
    23521 actggggttg agtcttgagc cgcgtcatgt gggacaactg agtttggccg tcttggcagt
    23581 cgtggcagcc ggacttgtgg gagagtgggt gttgggccgc cttgccgagc cactgaattt
    23641 aattagtcac tggaccgagt ggttcgatgc ggatctcgta tggggcacgc ctccgactct
    23701 gatcgtcagt ttgatggaat atgtggtctt tgcgccggtc tttgaagagt tggccttccg
    23761 tggactgtta ttcggcgtgt tccggcggcg gttccaatgg ggcacagcag ccatgctgag
    23821 cgcggccttg tttgccattg ctcacggcta tggattgatc ggattcttga gcgtcttctg
    23881 gagcggcgtg atctgggcgt gggcttatga gcgaacgggg agtttgtggc cgggcatgat
    23941 cggccatgcc gtcaacaacc tcctggtctg cctcagcgtg atggcgctgt tgcggccctg
    24001 aaagcggtgt tcgagcgaga ccactgcatg gttattccga tgggtagatg tgcggactgg
    24061 tacgtggtgg aaaggtgata acaacggtca cagccgccgg tgagccgtca cgatcagaat
    24121 ttgtgccggc cgcttgctga tgttgcgcca gcagtgccgg ttgcctgccg gtatcccggc
    24181 ggcatcgccg cgcttgagct ggtggacgtc ctcctccagc gtcaattcga gagatcccat
    24241 caggacgacc gcgagttgtt ctgcctgccg gacgtagggt ttatgaccgc tggctccccc
    24301 cggctgaatc gtgatcacca taggctcgaa ctggctgtcc ttgttgaacg ggccgagcgc
    24361 ttcgattttt gcacgagacc actcgctttg gacgaccggc cgagaatcgg accggatgat
    24421 ggccggaggc gtcgggtcga tggcgcgaaa gaactctccc agggtgacgc ccaatgcgga
    24481 ggcgatcttt tcggtggagg caatggacgg cgaagcctgg cgtagttcga cctgagagat
    24541 gaagctggga gagaacccgc acttgtcggc gagtgttcgc actgaaagat gatgagattt
    24601 gcgaagccgc cggacaatgt cgccgacgtg cacctcggct ttggtggcag gaggtgaggc
    24661 gcgtttcagt ggcttcgttt tcttggtagc cgtcatttgt ccgttaagaa cacatacata
    24721 gagccgagtg cgaaaggccg gctttttacc tgtgtaacac gtttttgtgg ggagtctcaa
    24781 ggatgagtct cttcccccgt cctctgcgct actgccgtct gtcgatttct cgacaccgcg
    24841 tcctagggac ttccccagct tcccagacct tcgtaatcgg gctaaggaag tgtccagtat
    24901 agctggacag tcgttcagcc attattgtgg agtgttcgtt gctcgttcgt cagtcactga
    24961 tggcctgatc tgaaccagga ccagtgacac atccagatgc gatgtgggga gggagcttga
    25021 aagacaaggc cacatggttg acgttgacga tatttgtaag tgcatgtgcc ctgttatggc
    25081 tgacaggcgt gaccagcacc aagcatctcg tggataacct cattgcgggc tatttgttgt
    25141 cttggggctt gtatgctttt ctctccgatc tgccgcgcag ggagattcag ggacggttca
    25201 ttttgatgac cctctgcggg attgtggtgt tggggacagc cgaattgctc ggcgtgacgg
    25261 gactcgtgaa ttaccaaatg ctgtttggga cccaggggcg tgaatggttc gatcgctccg
    25321 gatatgtccg tgatccggaa cttggatatc gccgtcaggc gcattaccag gaggaagggg
    25381 cgtttgtgcg aggaaatatc ggtgaagcgc tctgtcttcc ggcgaatgca cctcaacgat
    25441 ttgatctccg gtatgaccat cgggggtttc gcaacgatac ggatctggat cgggcggagg
    25501 tggtggtcat tggtgattcc tatgtggaat cgcccatgct ccccggcgac agtcttttga
    25561 cgacgcaatt aagccatcgg ctgcgcacgt ctgtcgcgaa tttagggatt agcggatatg
    25621 ggccggagca ggaactcgtc gtgctgaagc ggtatgcgct ggaccttcag ccgaagacga
    25681 tcgtgtgggt attttttgag ggaaacgatc tctatcaatt ggatccggaa gagcaaaacc
    25741 tgagcgccgt gcccgacacc agggccgaac acgacgagta ttggatgcgc tcgttgacga
    25801 ggaacctgct gctcctttca cgtaaagcgg tgcagggatg tgtgccccat ccagattact
    25861 tgcgacttcg cggaacgttt cacgatacag agggcaatga aaccctgttg tatttctggg
    25921 aaaggctcgg cgaattaaaa ccgacggaca gactccgcct cgagcggctg cgatcaattt
    25981 tgaccgaggc gtatcacgtg agtcgtcagc aaggtattcg cctcgtggtg gcgtttgcgc
    26041 cggtgaagca tcgggtgcat cagggattga aaaacttcga gccactcacc gcggaaatga
    26101 gagagtggcc gctcaatgaa ctgcccgatg aagtggcgtc gatgctgaac acactctccc
    26161 cggatatcga attcatcgat ttgactccgc cgcttcgcga ggctgctgca cagggtgtgt
    26221 tgacctatct tcccgatgac actcattgga cggcggaggg gcagcgagtc gccggtgaag
    26281 cgatccatca tctccttgct ggcggccaga ctcgcgtggc taagcagtaa ggcgtggcta
    26341 ggggcagttc ctgacgcctg attcctgctg gagggtcgcg agtgtgcgat agagatccgg
    26401 atgccggagg gtgtagcgta agccttcgag cgctttgcga ttgttaatcc gcttgcccgg
    26461 ttggctggag gtggcctccc gcgggctcac aatgccccat ctggtcgaca cttctacgca
    26521 aatctcggtc cagcggcgcg gaagcccgtc gctgatattg tagatgtctc cggcgcgtcc
    26581 gatttgcagg gccagaaggc agagcgcggc aagatcttcg acatgaatca agttcacaaa
    26641 tttattcgtg gggccgacgc gcccttggcg gatccactcc accggattgc gatgggggcc
    26701 atagattccg gccacgcgga gaatgatccc accatgcttc gtgcggaggt attcctcccc
    26761 ttggacccgt ggaaggtcga cattgacggg gcacgtctcg tcgatccatg ggggcatgtc
    26821 tgtcaaaggc tcatctcctc gatcgtaggc ggacgtactg cctaacacca ccaagcgtcg
    26881 cgaatggctg caggagtgtg tggcgaaggc cttcacctgc tcaagaggaa ctgccggaaa
    26941 cgtccagatc aggtccgcgt ccaacggcaa cgcagaccat gtggaaggac tctcgagatc
    27001 gaaccggatg cgggcagtct gggctatgtc gctgaggtgg tgctccggct ggcggctgct
    27061 ggccagaatg ggcagcgatt gttcgcgggc cagcttctga atccatcggc cggtgtagcc
    27121 ggttccgaga atcactatgg ggcggagcga gggcatccgg gcatcatacc cgaactgccg
    27181 gttcgtttgc tgctgccggt ttgagcctat tctggttcta gtctcgggac agagtcgcat
    27241 gctaaaatcc ggccccgatg gcgtctttca acttacgtca ggacgagacg ggtgacatga
    27301 tcggccgacc gaccgatgcg caggcgcagc ggcggcagcg gatcctggcc ggacttgccg
    27361 gcctgctggc ctgtctcttt tttgtgctgg atcttcagtt gccgctgggt gtggccaacg
    27421 cggtactcta cagcgcggtc gtgctcctgt cgtctgccag tcaatatcga tggttgccga
    27481 ttgccactgc caccgcgtgc tcattgctca cgatcattgc cattccgttc agtccgcgga
    27541 tccccaatct tcccccctgg tttgaatggg tcaatcacct gtttagtctc ttcggcgtct
    27601 gggctcctgt ggtgtttgtc tatcagcgtc gccgtacgga gcgactgctc gaagaaatca
    27661 acgagcggct ggaacgaggg gtagaaacgc ggacggccga tctggccgcc agccggctgg
    27721 cgctggagcg gagcgaagaa cagttgcata cgttgaccgg ccggattctc acggcccagg
    27781 aagaagaacg ccggcggatt gcccaggacc tgcatgatga tgtcaatcag cggctggcgc
    27841 tgctcatgtt ggagcttcag agcgttgacc ggcagattgg agtatcaccc gtggcagccc
    27901 aggacgggat acacaatgtg ttgaagggcc tcgaggctct gtccgacgac gtccgctaca
    27961 tggcgtatcg ctttcatcca tccattcttg atgacctggg tctgaaggcg gccttacagc
    28021 gcctcttgga tgatttttcg actcgaacgg gcgtcaaagc cctgttcgtc catcaacctc
    28081 ttgaccaccc cctcgacaaa actgcttcga cggcgctgta tcgcgtcgtt caggagagcc
    28141 tgtccaatat cgcccgacac gcgaaagcga ctcgggtgga agtggaggtg acggtcgaag
    28201 aggagggggt cgtcgtagtg gtacgcgatg atgggaaggg atttgatcag ctgacggtag
    28261 aacgatgtga gggcgggttg ggtctgttga acatgcgcga acgcctcctg tctgtacaag
    28321 gcacgtgcga agtcgaatcc agcccaggga agggaacaac cgtatccatg tacgtaccgc
    28381 ttgtacgggt ggcaatatga cgcttccgcg tgtgttattg gcagatgacc ataccctcgt
    28441 cctcgaggga tttcggcggt tgttggatga tcagtgtgaa ctggtcggca ctgtggggga
    28501 tggccggtcg ctgctggagg ccgtgccgca gttgaagccg gacatcgtca ttttggacat
    28561 ctcgatgccg gtgctgaatg gcatcgatgc cgcccgtgtg atgaaggtca agtttccaca
    28621 ggtcaaagtg gtgtttatca ccatgcatgc cgatccggcc tatgtgcggg cagcgtttga
    28681 ggccggcgcc tcggcctacc tgctgaagcg ctgtgtggga gaggaattgg cccaggcgat
    28741 ccgggctgtg cggagcggca atttctatgt gacgcctctg gtcacgaagg aagttgtcga
    28801 aagcatgctc cgcggcgtgg acggcggagc tccatccggc cctgagctca ccactcgtca
    28861 acgcgaagtg ttgcaactcc tggccgaagg ccacacggtg aaggacattg ccggcacgct
    28921 caagatttct cctcgcacgg ttgagtttca caaggggcaa atcatggaac agctcaacct
    28981 gcataccaca gtggaactcg tgaaatacgc cctcgcgcag ggtctcacca gccaaacttg
    29041 atctcctgct cggtttccgc ccccgtcttt ctctgctacc agtatttaag ctagtatatt
    29101 tttgatgtaa aacctcgctt tgttacgcgt gtggtttacc cttcacttgc ctactatacg
    29161 tatccctttt ctaagcagag actcgtagga ttatgagaaa aaagatttcc cgtccccgtg
    29221 tgctgttagg tgattcctca cgttccatgt tggcctttct cgagattctc ctggagtctc
    29281 atttcgatgt cgtcggctcg gaaaccgaag gcgacgcctt ggtcctgacg gcaaaaacac
    29341 ttgcccctga gttagtcatc ctggattttt ccctctcgtt cctcgatggt ttgtcggtcg
    29401 gccggcagct tcaggaggct gtgccggaca gcaaggtcgt gtttttttcg tctcacgaag
    29461 atgccgccta tgtgacggcc gcctttgagg tcggcgcgat gggctatctg gtcaaacacc
    29521 ggacggtgga ccccggccat tatctgcgcc gggtgctgga cggggagcgg gtctgttgtc
    29581 ccgacgattt acacaagcgg gtggggcgcc ccaaaaaaaa gtagtgccga ctccatgcat
    29641 gcagtcagct tcgcgcaaaa ggagaccgac gatggtccga gtcgtaccga tgtgtgagtt
    29701 gtgccgtcgg gttcgtgacg atggattttc cgccagggga acgaattgtt gggtcgattt
    29761 cccgagttac ctggcgcggc atgtggtgtc gccgtcccag gtgcggtttt cgagaaatta
    29821 ctgcagcgaa tgccgcttgt cgtatgagat cttaaaatct tatggcgagc agtgagacgg
    29881 ccggcctgct gatgatccgg cttagatcgg gccgacgaag cctcaagggg tgaccccggt
    29941 gctcccccgc acggtccccg tttgtaccgt cccgcccccg tcgtcttcct tccggccatt
    30001 tcaaatctca acggtttgac cgcatgttca gctcgtgacg gttgtgttac gatcgcccga
    30061 tgacgccggt tcttcccttt catcctatca tcgccgactg gttcacggcc aaatttgcgg
    30121 tcgcgactga cgttcagcag caggcctggc cggagattca gtcgggccgt gatctgctga
    30181 tttccgcccc caccggatcg ggaaagaccc tcgcagcatt tctgtcctgt atcgatcggt
    30241 tatttcagca ggcgctgcgt tgtgacctgc gggatgaaac acaaatcctc tacgtctcgc
    30301 cgctcaaagc gctcagcaat gatgtgcaaa aaaaccttca acaaccgctt ctggagatcg
    30361 ggcaggctgc cctctcggcg ggactgttgc tgccagacct ccgggtcatc gtacggaccg
    30421 gcgacacacc gatgagcgag cggcagcaaa tgctcaagcg tcctccgcat attctgatta
    30481 ccacgcctga atccctgttt attttgctga ccgctgaaaa aagtcgggcg atgttgaaga
    30541 ccgtccgaac ggtcattgtg gacgaggttc atgccgtcgc cccgaacaag cgaggggcgc
    30601 acctggccct gtcattggag cgcgtggcgg cgctggccgg tggtgccgtt caacggatcg
    30661 gtctctccgc cacccaacgt ccgattgaac ggatggggca cttcttagtg gggaacaggt
    30721 cgtcctcccc acagacgcag gatgagcatt ccgcagccgg gtgttctggc gacgagatgc
    30781 cggtggcgaa ccatgtggcg atcatcgatg tcggccatcg ccgcgacatg gacttggcgg
    30841 tcgaagttcc gaaggacgaa ctcgggcccg tcgcgaccaa tgcgatctgg agcgatatct
    30901 acgatcgggt tgccgcactg gtggaagcgc atcgttccac actggtcttc gtgaatacgc
    30961 ggcgcttggc cgagcgggtc tcgcactact tggaagatcg gttgcgtcac ctgggcgaag
    31021 gtgtggtggc agcccatcac ggaagcctgt cgcgggagat ccgcctgtct gcggaagacc
    31081 ggttaaaaac cggagccgtg cgagtggttg tcgcgacggc ttcgctggaa ctcggcatcg
    31141 atgtcgggac ggtggatctc gtttgccaga tcggatcgcc tcgctcaatc gcaacgtgtt
    31201 tgcagcgtgt cggccgtgcc ggccattggg ttcatgccat cccgaagggc cgcctctttg
    31261 ccacgacgcg tgacgagttg atcgaatgtg ccgcgttgat ccgttcgatc agagctggtg
    31321 tgctagacca cattgaggtg ccgcccgcac cacttgatgt cctggcgcaa cagatcgtag
    31381 cggcatcggc cactcaggcc tgggatgaga cggagctgta taagttgtgt tgccgggcca
    31441 tgccctttcg tgatctcacc cgcgagacct tcgacgcggt gattgccatg ctggccgacg
    31501 gatttgcgac cagtcggggc cggggccgag cctatgtgca tcacgaccgc atcaaccatc
    31561 agattcgcgg gcgccgcggg gccagattgg ccgctattac ttcgggcggc gccattcccg
    31621 acacagcaaa ctacgcagtc attgccgaac cggacggcac ggtggtggga tccgtcgacg
    31681 aggacttcgc cgtcgagagt ctggcgggag acattattct attggggaac acgtcctggc
    31741 gcatcaaggg cgtcgaaacc ggcaagatgc gtgtcgaaga tgcgcagggt gcaccgccga
    31801 ccattccgtt ctggcgcggg gaagcgccgg cacgcacggc ggacttgtcg gcagaagttg
    31861 cgcgcctcag agccgatctc gaccatcgtc tcgatccgaa tgtgccggcg cctcctcctt
    31921 ccgcgccgcc ggtgcaatgg ctcaaacagg aatgcggtct cgatcagcgg ggggcggagc
    31981 aagcggttca atatatcctg gccggcaaat ccgttctggg gacggtgccc acgcaacaca
    32041 cgatcgtggc ggaacgattc ttcgatgaaa gcggggggat gcagctggtc atccatgccc
    32101 cgttcggcgg cagggtgaac cgcgcctggg ggctggccct gcggaaacgc ttttgcgtga
    32161 ccttcgattt tgaactgcaa gccgcggcga cggatgaggg gatcgtactg tcgctgggcg
    32221 aaaagcacag ttttccgctc gagacggttt tcgcctttct gaacgtcaag actctgcgcg
    32281 acgtgttgac tcaggccgtg ctgcaggcgc cgatgttcat gacacggtgg cgatggaatg
    32341 cgtcccgagc cctggcgctg ctccgatttg ccggagggaa gcgggtgccg cctcagattc
    32401 aacgtatgcg cgccgaagat ctcctcgccg ccgtgttccc ggatgccatc gcctgccagg
    32461 acaatttcca aggagaacgc acggagcgcc agattcctga ccacccgttg gcgcaagaga
    32521 cgatccggga ttgcctgacg gaagcgatgg acattgacgg gttagcggcg gtgctgaacc
    32581 gaatcgaatc cggcgcgatt gcctgcgtcg ctgtcgatac gccgatgccg tccgcttttt
    32641 gccacgagat tttgaatgcc aacccttatg cgtatctcga cgatgcgccg ctcgaagagc
    32701 ggcgggcacg agccgtggat atgcggcgca cgcttccggc tgcactggcc ggggagatgg
    32761 gcgcgttgga ccagtcggcc atcgatcagg tcagagaaga gtcctggcct gatgtgcgtg
    32821 atgcagagga attgcacgat gcgctgttga cgctcggctg ggtgccctgc gcctgtatgc
    32881 cgggatggga cttgctcgtg ccgaggctcg aggtcgcagg ccgggtcgcc acgctgtggc
    32941 ggggtgataa caaacaggga tgggtggccg ccgagaatca tcagtacggg ggcctgctct
    33001 ttccggatac ccggatcgat ccggaatcgg caccgactca tgagcctttg ctggaacggg
    33061 aagaggtgtt gcagcgcatt gtcttaggct ggatggaaag tgtggggccg gttacggccg
    33121 ctgatctggc tgcgcgtctg ggcctgacgg tgccggatgt cgacggcgcg atgttgcgac
    33181 tcgaagcgca gggccacgtg cttcgagggc ggttttcaac gcaggtgtcc cacaccacgg
    33241 atggggtggt ggaatggtgt catcggcggc tgttggccag aattcaccgc ctcacggtcg
    33301 gacggttacg caaggagatt gaacctgtca ctgccgccga gttcatgcgg tttttattcc
    33361 agtggcagca cgcgtcaccg ggggcgcgtc ttcatggaga ggccggactc ttggaggtgg
    33421 cgaagcagct cggagggttt gaagctgccg catcggcctg ggaggcgcag attctccggg
    33481 ttcgtttggc caagtatcaa ccggaatggc tggaccggct ctgtttgggc ggcgcgctga
    33541 tgtgggggcg tatcacgccg catccgcggc tcatgcagga gctcgccctg ttgccggggc
    33601 ggcgtgtggt tccgacgcgg gtcgcgccgg tcagtctgtt tgcgcgagaa gatgctccgg
    33661 tgctgttgga ggcgactggc gaggatttgg cccgattgga tctgtcctcc aaactcagcg
    33721 tctcggctca ggccattcgc cattgtctgc agcagcgtgg tgcgagcttt ttcagcgagc
    33781 tgctgcatgg tgcccgtttg ttggcatcgg aagtggagga tgggctctgg gaattggtcg
    33841 cggccggcct ggtcacggct gacgggttcg acaatctgcg agcgctcatc gatccgcgcc
    33901 gccgtcgggc ggaggcgtcc gatcggaatc gccggcctcg ccatgtcggt ggacgctggt
    33961 cgcttttgag gccggccgtc ggacaatcac agaccggtcc tcaggccgcg acgaccttgc
    34021 gagcatcgtc ttcgtcggcc catgttgtgg gttcctcaga aagggcgcag gccaccgaac
    34081 gtattgcgcg gcaactgctg cagcgatatg gcgtggtatt ccgggatctg ttggcgcgcg
    34141 aatcgatcgt ctcttcctgg cgggatctgt tggtctgtta ccggcgcctg gaatcgacgg
    34201 gggagctacg cggggggcgg ttcgtcagcg ggttcacggg agaacagttc gccttgcccg
    34261 aggcgctgga agcattgcgg gcattgaaaa agcgtccagg taccgccacc cagcaggaga
    34321 tcaaaatttc ggccgccgat ccgttgaacc tggccgggat cattctgccg ggacctcgta
    34381 tcgcagcggt cccgtcgaac tttgtggtgt ttcgcgaggg cgtcgtcatt cgcaccgtga
    34441 ccgggagaag tgcgactgat cggcaggagc cgcccatcct cgaagtggca caacgggatc
    34501 tgcgatcctg atcagataag cggcgcgggg atggcgagca acgggcatcg cgctccgcgc
    34561 aagacccgtt ccgtggtgct gccgcgtaac atgtcgagca agctgtcctg ccctttggtt
    34621 gccatcacaa tcacatccac atcgaattcg gcacctgccg cgagaatcca ttcgacggga
    34681 tcgccctttc caaacaactg attccaggac cagtccgcct tctgcggaag cgccagtgtc
    34741 tcaacatcgg cttccgcgcc tgcatggata agcgtcatcg tgaccggtgg cttgctcaat
    34801 tgggacacca gcacggtcgc cgcatcgatg gcgggttgag aggggggatg gctcgagatc
    34861 ggcagcagca cgcgatgcag tgaggtttcg ccggtggtgc gagagacaaa tcccgcgacg
    34921 ttggatggga cgaacagcgt ggtgacatgc atttcgcgcg aaacgggctc agcgaccgcg
    34981 tgatgaatcc atccggcaaa cccttcgtgc ttgtgcgtcg ccagaaccat gagatcggtt
    35041 ggtgtcgcgg tcaaatgatg catgatggcc tgcttggcgt cggccgccag ggcgcgaact
    35101 tttctgatcc ggatgccaag cgtggtcaca tcgcttttgg aactggattc cggaaggagt
    35161 ccccactgcg cgagggtcgg acggatgcgg ggaaaatcct cgaacccttc cggcgagacg
    35221 tcgggatcca catgcatgat ggtcagctcg gcgcggaaga ccaacgccag tttgagcgcg
    35281 tggacgaatg ccacctgact gtcctgcgaa aaatccgtgg ggtgaaagat ccgatggatc
    35341 gagaggggat tgcgcggcgc gttcatgtcc gtatcgaact ccggtgaggt gcaatctgcc
    35401 tgtgctgccc ggtgatcggt ctgtgtcgtc agcggtgcgc ggtttgtaaa gccttgacca
    35461 gctcggtggc tgacgaagag ccggcctggc tccagcggct gaacgcagcc tgcgccgtcg
    35521 gttgaaacac agccgcatcc ggtagatggg cgagggcgtc caacgacgcc agatattcgc
    35581 cgtgaccccg cgccgcatca gcttccaaat tgtctgcatt atacgcggtg aaggcggcta
    35641 ccttgtgctc agatttcagc aggccgtctt cattccacca gatgcgcccg gacgtggtgc
    35701 cggtgatatt cgatgtggtg tcggtggtct ctttaaaggt ggccttgaac gaacaggctg
    35761 agagcaacgc caggagtgaa agtccgctgg ctgcggcgat gagcgaacga cggtgtccag
    35821 tcatacgtag tgctcctttc atgcggccac atggagtgat ggtggccgac tatagcatag
    35881 tcgacacgga aatgagccta ggccggccct tggtcgtcag aggttgagtc tgttcgaagg
    35941 aggtaacggt tgacaatatc cccaggcatc agcacaatcg ccgggtcctt gatggggcac
    36001 gaattcgggc gaggcggcct ggtcgctgtg atgagccagg agcagacgca ccccggtatc
    36061 acaaggaggg gcgacgatga aggtaggcat gggcttgtgt gtgatcgcag gaatcctctg
    36121 tgcagcgcca ttggtgcggg ccgagacggt tccgctgaag ccgggtgagt atcaggtgac
    36181 agccgtgacc gagagcagca gcggggaagc cggcaagccg gacacccata cccgatgtgt
    36241 gaaggaagaa catctggcga acccggatgc ggtgtttaat tattatgcct taaacggatt
    36301 caaacccaat ccggccaaca aggtcatgaa cgtgtcgatg catggcggga cggtcagtta
    36361 cgatattgaa ggcctctatg cgatgacccg cgtcgaaggc acggtctcga ccaccggttt
    36421 ttctgtggtc cgcaaagcga cccctaaggg cgacaaggcc ctgtcggtga cgatgaaggt
    36481 cgatggcaaa cgcacgggcg attgcgcgac gaagtaagtt cctgcccgat atcgcatctg
    36541 catacagcga aggtggcgtg acggaggccg gcgttccggc ctccggtttc tctcgctgct
    36601 agttgtccca caaacgctga taccacttct tctccttccc ggccggaaga gatgccaatt
    36661 tcatggtgtg gcggatctcg ccgatattcc acccggtcag cgaggcgagt gtctgcgctt
    36721 cctgctcgta tcgctcgcgt aattgttggc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36841 nnnnnnnnnn ncagggatca caccagttca tgtcccaggc atattccatg aagatcccgc
    36901 gttcgctttc ccgcttcacc tgctggctga agaggtcgcg gtagaagtcg ccgaactttt
    36961 ccttcacgaa cagtggaacc tcctgcgcct ccggtaaccg caccgtccgg tagtttgtgg
    37021 tttcgacacg gccttgcttg gtgaggaagt agaggaacaa ttcctgcgca ccgtcggcgt
    37081 tgaccgtgcc gagccggatc ggcagcatga acttgggcga ttcgaacgcg atctgcagcg
    37141 gccggagata cgtgaggcca agcttggctt gttcaccgag gttgaccttc gcgacgaaga
    37201 atttcatgcc ctgtttgaga taactctgca ggactgccga ggcgccttgt gggatgcggt
    37261 agccgttctc ggtcaaccag atttccaggc cgctgctttc cttgcccgag agaatgagga
    37321 tgtcgtattc accgaccgta tactgcgcct ccacggttac gcccagggcc ttgtcacgtt
    37381 cacgtggcgc ggctgacggt gccacgctct tcatggcgtc catgctgcgg cgttccatca
    37441 gctcatagcg gaggcaggga ttctgatcga agtattccac cagccgaggc gccgagtaat
    37501 ccgctagatg tttcagcagg gccggatcgc ccacatggat ctgctccttc tccagaacag
    37561 tcgggaccgg caccaccagc gcgaactcct tcacctcgcc cttgaagtca ttggccatcg
    37621 tgatcacggt cttgtcgtcg tgccgcgcga tggccacctc ggaggccttg ttgaacagtt
    37681 tcgtatcggc cttaccaaca tagaacccgc agaaagctaa ggcgtcgcca ccccaggcga
    37741 gcaggctcag ggtgaataat actgacggaa tgatggcacg tcgcatagaa acctccttgg
    37801 ttgatgacag aacagtggag agagtgcgga ctggtattgg agccgggtga ggagagccac
    37861 cggttgaagc gtgcgcccac tggtagcgcg agccggggag tacatgatcg ataaccggca
    37921 ccaacggcgc gcaggcaatg agtccccaaa gcggcccgtt gggcttgaat agtccgaatt
    37981 gcacatagag cgcggtgaaa gacacgagga gtgcgtagac catacgtccg atgcgtgaat
    38041 ccggcgtggt cttcgggtcc gagatcatga agaaggcaaa gatgagcagc gtgccgctct
    38101 cgatctgatg gagtggaatc gtgaggggat caccgagtcg aactgcccgg gcgaacagga
    38161 gcccgacata acaggcgagg aacgccagcg tgacatcggc gcgtgcggcg cgggtgacca
    38221 ccagactccc gaggcaggct atgagaaagg cgagccaggc ggtctgcccc cattgccccg
    38281 gcgagatcca tcccagcccg ctgccgagca tgacggtcaa ggccagattg gtcggattga
    38341 agaggtgctt gtcatgccag cgaaggacaa acttgctgcc gatggcgagg aaggccgcca
    38401 atgcggcgac cgcgagatca tcggtacgca ggaggaggca cagtgacagt gaagagatca
    38461 gtgcgctctt ggcatcgaat gccggaaggt tgacgaggat ggtcccgaca tattgagtga
    38521 gcaatgcggt gccgatcgtc accacaatct gccggatcga cagatcgaag tggagccagc
    38581 acagaccata gatgaggaga gtgcccaggc tggcgatctg gtagtagcgg ggatcctgcc
    38641 gagatgctga cgggtcaggt tcaggattgg cgtgctgctg ctccataact cctcctttac
    38701 ggagggtatg gcatgactcg gaaaatcctt tcagcgggcc atctgcctgg ctctagggat
    38761 gtcccccatt gagtagatgg gcgtaatcca ttagcatgaa cttgaatcca tcgttcagcc
    38821 gaggtgatgc cgtgcccatc caggttattg ttcttatagg gtttttgtct ctgctccccg
    38881 gctgctcgct cgaacaagag ttgtccagaa cgcccaggac ggctgtcgag caagtgctgc
    38941 tgacgcaggc tgtgaatcaa gcgctcacga atcttaccgt gcaattgcct gtcggtgtga
    39001 atgttgagct cgatgcgacc ggtctggaga gtgaccggtc ccgacttcgc atgacgaatt
    39061 cggacctggg ctcgttgaac agtcccaacc gggacattct gtacatcaga gatacggtcg
    39121 cggctgaatt gggacgcaga gggtatctgg tgcgggcgcg cgacacggag tcgccctatc
    39181 ttgtgcgggt catggcggaa tcgttcggga cgatgcaggg gatcacgttc ttcggcatgc
    39241 cgccggtcca gagtgtgctg attccattcg cgcttccgga attgacgctc tacaaacaac
    39301 aaaaccaaag cgggtatgcc agactccatc tggatattta cgacaatcgc acgggcgagt
    39361 ttctcggttc ttccgccact ctcgtgggtc gaacgtttta caaccaatac acgatgttat
    39421 tctttctcac ctggaaccgc accgatatcg acgcgccgcc gtgatacgcg agagccaccc
    39481 ttccttgcaa tccttcgagt ataatccggc ccatgcttga actgctcctc gtgctggcga
    39541 gcctggtggt gatcgggctg acaggcctcc tgacggttgt cgccactccg ccccgaatgg
    39601 ttgagctcgg cctttgggcg ctggccctcg gcttgctcat cggtatccca acggggtggt
    39661 ggtatcacgt ggtgctctat cgagccttgt ccgcgcgcat ggcgctgccg cgtcggtggt
    39721 ggcaaagacc ggtagaattg catccgttac tgcagccgga tgaatttcgc cgggtgcgtc
    39781 cctggtttgt ggcgggtgca ctgggttttg ttttgtgctt caccgggggc gtggcggcga
    39841 tagccggaat gctggtgatg cggttctacc cgtgatgccg acagtcggca atcctcacat
    39901 tactgaacga ccggtatctc gacggcgagc tgggcgctgg tggatggatc ctggctcaca
    39961 acgtaggtcg cctgttcctg aacgcctgcg gttgtgccgg gagagacagc ttcgatgagc
    40021 agtgacgcgg agaatcggtc cagcataccg acttcctgca tcgtcaacgg tccgaagggt
    40081 ttcgaagata gccggataat gagcgtgacc agcccgtttt cagcgggagg cggtatggga
    40141 atgtcggtcc gtttcccagg ctggagtttg agggaaatct tgccggtttc ctgcggcggc
    40201 caccagagcc tggtgccggc agagccaagg ttttggagca gctgtacgta cgattcctgc
    40261 gtcgcctcca cggtgagacg gacggggtcg ctgcttcgcg caaccatcgc tgccgtgatt
    40321 tcctgagtcc ggccatcggt tcccctcgtc acaaagctgt agcgaaggcc caacggtttc
    40381 gcctggccga ggctggggct cgattgctcg ccataaaaga gggcgcgggc gctcttcttg
    40441 ggggctggag caccagccgc cgaaaatccg gccggcgctt gcaagagagg cggcgctgcc
    40501 gatggttgag gcgctgttga aggtggtgcg ggggcagcaa gcgttcggtc ggctgaaggg
    40561 ggcgtgccgg tcgatttctt ttcggcagcc gccatcgggg cgttgttgct ggattcatcg
    40621 cgctcacgtc gagggggagc cgtatcagtc gaagatttgg ccgcagactg ttccgacgcc
    40681 ctgcccgcaa gaggctgctc gcgtttggcg cgcgtgtcgg tgcgtctgtt ttccttggcc
    40741 gcgtcggcag gttccatggc ttgcgggtcc ggcgtagcgg cagactgtgg cgccggcatc
    40801 gacggttgta cacgtggcat cggcggtgcg gccggtttaa cctcctcagt cacgatggat
    40861 tgtctcgctc ggtcgaggct ctcttgatag attctggtcc cgaatgcgat ggcaaagacc
    40921 gcagccgcca accctcctgc ccaggcgagc cctgccggtt tcttcagcca gccccaccag
    40981 gtgtctgttc cgccggtagc ggtggggctt gtgtgctgca atgcctggag gagccgccgg
    41041 cgaacatccg gattcgccag caactctttc aacgcctgtt catcggccag ggcagtgaag
    41101 agctgttgat cgtgcaaggc cgcggcatag agctgcttgc gttcctccgg cgtgagggta
    41161 ttcgctgcaa agccgcctaa gaggtgttcc aattcgtgat cagccatgct cgatacctgt
    41221 cacaacgcga tctctgatgg agtgagcacc catcatcatt cccatgtccc gcccatcaga
    41281 ctcaataact gtttccggca acgcaggtcc caggtgtaaa tggtattgat cgacgcctgc
    41341 cccatgagcg tttgaatttc agggaaactg ttcccctcca gcttccattt gaacagttgg
    41401 cggcagcggt ctccgagctg tcccagagca gccatcaagc gatcgacacg ctgcttctga
    41461 tccaattgcg aggccggatc ggtgccggga tcggccgacg gcaggtcatc gacggagtgg
    41521 tcattatact cgccccgccg cagtgatttt cgatgggcat ccagcatttt gaagcgcagc
    41581 acttgaaagg ccaggggcac cagttcggtc aattcagtca cgcggacata tttttcatgc
    41641 aggagcatca gcacctcttg cgtgagatcc tctgccacat ccttctgaag gcgtgatgtc
    41701 gcgaaggtca gaatcctttc gcgcaggtgg gtgagaatct cgtctcgcgt cacggcggtc
    41761 gtgctcagtg aggtgtctgt ccggatgcat ggggaatgag atccccgatc gaccggaacc
    41821 ctacctgaag tccttggcac acgtccacct gccgatcgaa gcggttcttc gtcaggatca
    41881 gttgtcgcaa ccttcccatc gcgacccgtc cccatgccgc caggtgaaag acaggcaacg
    41941 gataatcggt tccgaacagc agacgttcat ggacctgcgg atagttccgg aggtgcagca
    42001 gcatccgtaa acggttgggt aaggtgaggg cggaaatatc cgcataaaag tgcggataac
    42061 gctgcaccag atcatgcaag gtgggaagaa acttctcata gaggatcagg ccgtaactgc
    42121 acgcatgcgc cgcaatgacc gtcacgccct catccaatgc gacgcgaagc cgatcaggct
    42181 ctcccactga ctggtccttg ccgatcaagc tgaattcata gccgacatga ctgagaaacg
    42241 gcaacttccg ttccgccagc gctcgataaa agggggtgta tgtcttgttc gccggatcga
    42301 actgctgcgc attcgccagc actttcacta acacagcacc ggcatccgcg cagcgatgca
    42361 cttcctcgat ggcatcgcgc cgttgggggt taatggatac gccggccagc aattcatcgg
    42421 gatgcgcctg ggccgttttc aggacatagt cgttcccgat gagaaaatcg gtgttcgcct
    42481 ggttgagccg gccgttctga tcatacgcac cgtccatgcc gaggagcacg gccttgtgca
    42541 cgtggcgtga ggctctcaat tcagtcagga gatccgcgag atatttctgg ttcgcttcac
    42601 gcggatgctt cggcgagagg ccatgtttcc agagcaggaa gcgaaacagc gggctgcgta
    42661 gcgtttttgg agaaatgtag catccgttgt cgccgtccgg cagcgccgcg agatgaacgt
    42721 ggcaatcgat cagcgttttt gtgaccggtc ttgtcatggg ttcatcaaaa actggtggct
    42781 cctcggccgt cacctggccg gaacctccgc gaggatggtt cacgttcccg atatgttgag
    42841 tcgttccatt gccatccgct tgagggcgcg gagatccaat ttgcccgttc caagaatcgg
    42901 aagcgcttcg accttgaagc aacggctatg ggacggcaga aacaaattcg gaaggccggc
    42961 ggcttgcgcc ttcgccaaga tgtgcggaat ccgttcttcg gcgagcgtat ggaggacagc
    43021 cagctgttcg ccttttcgct cgtcgggcac accggtgact gcgcagactt gtaattcttc
    43081 ccctgaggcc tgttgcagcg cgtcttcaac caaccgatgc ggcaccattt ctccgccgat
    43141 cttggaaaaa cgtgacagcc gatcggtgat cgtcaggaag ccgtcatcgt ccagcgtcgc
    43201 gatgtcgcct gtaatatacc atccgtcgcg catggcctga gctgtcagat cctcgcgccc
    43261 taagtagccg ttcatgacgt tcgggccttt gaccagcaac atgccgggca tgccgggcgg
    43321 aaggatggcg tcagtctcgg gatcgacgat acgaacggac acgccgggaa gcggctggcc
    43381 gacggtcccg cgccgcgagg ccggttggaa aaatcctgcc gctctgaaat ccggacagtt
    43441 cacggcaatc acgggcgcgc actccgtcac gccgtacccc tcgatcgggc cgatgccgaa
    43501 ccggtcctgg aacgattggc agagacgcag cggcaatttt tccgcgccgg tcagcacgac
    43561 tcgcagcgtg ctgaattgtt cgggcgtaca gcggcgctgg tagagctgta aaaacgtggg
    43621 cgtacagacg agaaatgtga ggcgatattt ggcgcacagt tctccaacgg ccgtgacatc
    43681 gaggggagaa ggatggaaca cgctggccgc gccgttgagc gtgacatacc agaacacgag
    43741 gtagccgaac gagtggaata gcgggagaat gccgaggatg cgatcttccg ggtccagggg
    43801 aagcacttgg gagacggcct gcacattggc gtcgatgctg aagtgcgtga gcatcacacc
    43861 tttgggttcg cctgtgctgc cgctactgaa gatgatggtg gcgagatcat ccatggtcac
    43921 ccgccgcgtc tgtccgcagg acgattcaat gagccggagc ggagcgaaaa gcgccagcgc
    43981 tgcggcgagc attttttgca ggctgccgat ggtagcaccg atgtcttcca gccacaggat
    44041 cgttggcccg tcggggactt cgagcttggc tttctcgata aacttccggc tcgtgacgat
    44101 ggtacgcagt tgtgcctggc ggacggccga ttcgaggccg gccttcccga cagtgtaatt
    44161 gaggttgacg ctgctgcgac cggcgagtgc cgcggcgacc gtcgtcagtg cccccgcgac
    44221 agtcggcggc agcagcaaac ctacggacgg ttgtccttgc cagtggggcg tcagcgcgcg
    44281 cgccagggcg atcgcaccga tcaaagcctg gagggaagac acatggggct tggttgcgtc
    44341 ggccatagcg aggcgaaagg gatgccgacg catggcgcgg acaaaggcgg catgcaacgg
    44401 gcgtcgggac gattttcgcc aatgccaggc cgtctcgccg agttccctga cgagtcggcg
    44461 gagctcatgc gccggtgtgc cggcaggcac gggggcgccg aaagagacgg tcaccggata
    44521 gggtagccgt tccggccatt tggtcacaaa ccgtcctccg acaaaactga agatgctccc
    44581 ccagacgcga tccagatgca ccgggacgac gggcacctcc cggcctttca cgatccgttc
    44641 gaatccccgg cggaacggca gtaagttgcc ggtccgtgta atctgacctt cggggaagat
    44701 gcagaccagg tcaccgtggt cgagggcctg gcccgcatcg cgcaaggccc ggagaatgac
    44761 gcgcgggccg ccatcggagg caatcggaat gacgttcaac gcccgcatga acggtttgaa
    44821 gaagggaagg ttcacgtagt gcgcgtcgac gacgaagcgg ataggccggt ccacgctggc
    44881 gatcagcaaa aatccatcga tgaatgaaat gtgattgggg acgagcaggg cgccacccgt
    44941 gaccggtacg tgggcctggc cgacgatacg caaccggtag atcgtatgcg tcagcagaac
    45001 caggacgagg cgcaggaagg tttcgggcat cagccacatg gcccaggccg tcccgatggt
    45061 agtgaggccg gctgtcgcga gaaagatgcc cacggtcgaa agtcctacat tcgctagcac
    45121 accgccgctg agtgagccca gcagaatgcc ggtgaagacg cagatatttt ccagcgcgat
    45181 gaccgagccc cggcggtcgt gcggtgcgcg ccactgcacc agggcattga tgggaatgaa
    45241 gatgagagcg ctcgacatgc ccaacgcgat catcacgacg agggtaccgg tgaacgccgg
    45301 cacccaccag ccgagcgtca ggaggaaggt cgctacgccc gccgccccca atggaaccag
    45361 cccgtattcc acgcgagagc gggagagtcg tcccaccacg agggcgccca agccgatgcc
    45421 gaccgagatt agggccgaag gaacagtggc aagtgcatcg gaaaggccca gtacggcttt
    45481 ggcgtagacc agcacgttct gcacaacgag actggcaatc gtccagaaga acacggcgcc
    45541 cgtgatggcc agccgcaaca gccgatccgc gcgtaatgcc gagagggcgc cgcgcaaggt
    45601 gtcgaagagt cccccggcag cacgtgcagc cggcactggc ggcacagccc gggctgcggc
    45661 gaaaccgatc agcgccaata tggtcagcgc cagcggagca agccagggtt ggtttccggc
    45721 gccggccagc aggaagcctc ctgcagtggt gcccgtcaaa atcgccaaga aggtgaacaa
    45781 ctccagaata ctgttgcccc gcgccagttg ggcatggggc agcaattcgg gaagaatgcc
    45841 gtatttagcc ggactgaaca gcgcgctatg gacgcccatg caggcaagca tgacgagagc
    45901 ccaggcacct ccggtgggat gagacagcag ggccagcgtt gcaccggcca tgagcagcac
    45961 ttccacggcc ttcatggtga tgatcacggt ccgcttgctc agccggtcgg caaaaaatcc
    46021 cgccacgaag gaggtcagga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46141 acggtccgct tgctcagccg gtcggcaaaa aatcccgcca cgaaggaggt caggaccagg
    46201 ggcagggtaa acaccaccat ggccagcgtg gtctgcgttt gcgaggcggc ttcaagatcc
    46261 tgtccaggcg caagggttgt ggtgacggcc cggatgccca ggagggcgac cataaatttc
    46321 caagcgttgt cgttgaaggc tccgcaaaac tgtgcgacga gcaatccgcg caacggccgc
    46381 gcgggtggtg gtgaggaatc ggactgcgga tcgataggag cctccttgcc tggcgaaaca
    46441 caggcgcctg tcaacggtca tgagtccagt ctgctgaaac gtcctggaac gatgcaatga
    46501 cttaccgtgg cggcattgga tcggcgctct ccaccggaag cccttccagt gccatgattc
    46561 gtaaggcatt ggtcgtcggg cagggacccg gcttgagacg atagtcgaag tgcagggcgc
    46621 cggccacgac ggtttcctga aaatgaacat tggtgacagt cggtaactgc ttctccaact
    46681 cagccagttc cagatcgtgg gtcgtgacca gtccgaatcc attgcctccg gcaagcgcgg
    46741 taatcaaggc cttccccccg atgagtcgct cccgattgtt ggtgcctttg aacacttcgt
    46801 cgatcaacca cagcactgga ggtccttgtc tgtctctcgt gctgtcgagg atggttttca
    46861 ggcgtttcac ttcggcataa aaaaacgaaa ggccgccgtc gagagaatca tccacgcgga
    46921 tgcaactgcc gatacgtacc agcgaccagc gaaaagccgt ggcacacacg ggagctcccg
    46981 cctgcgcgag gcaggtgttg atgccgatgg ttctcagaaa cgtgctcttg ccggacatgt
    47041 tggagccggt gaccagaaag attcggcccc gcccttgcaa cgtaacctgg ttggccacgc
    47101 ggaccgcggc aggaatgagt ggatgcgcca gcccttcggc cgcgagttcc ggcgcgttgc
    47161 cgttttcttg accgtctgcc ccggcgtgat gcagcgtggg ccagagatag ttcgggtgca
    47221 aatgggcgaa ggtggccagg gacgcggcgg catccaaatc agcgagcaac tcgaaccaga
    47281 gtggtgcgac agaggcgatc cggtcctgga gttgttgcag gcgataggta tggaagagat
    47341 cccacggacc gacggcattg atgagatagt ggaccaaggg atgggcacgt acactgagcc
    47401 ggttcatcag ggaagcggcc tgtttcagtg aacgcgacgg actgctgtct tgttgcatca
    47461 atggagcgca gagcttggcc agatgcgggg tgcttcggta ggagcgccgt tcgagatact
    47521 gtagcacggc actgagccgc tccaattgta ggtgcaatga ctgggcatgg tcgaagattt
    47581 cttccgaccg gctgcggacc gacaggaaca ggaaggcgta caacccgaat gacagcatcc
    47641 aatagttacc aaggccggaa atcaagtcgg cgagcagcag gatggccgtg gtgacggcaa
    47701 gagccgattc cgtcagcaac atcggcatga gtccagaatc gtccacgcgt ttctgtaaca
    47761 ccgcaagcag ccggcgcccg tcgatgtcgg cttcctccac cgcctgtgcc tccaggacca
    47821 gtcggtcacg gagcagcgac aagggcgtca attcccggat gagggcctgc cggctcgacc
    47881 atggtgcggc aagaggaggc tgatccaaga accacgaggc gagacgttgc cgaccctgag
    47941 aggaaatgct gtggtcgatc aggtgcagca gagagcgggg accggtaagg tcgagatccc
    48001 tggcatatcc atgccgttcc ggcacggtca cctcgcgaag cggaatctcc gcccactgta
    48061 agctgaggcg tgccagatgc actcgtttga tgccttgcca gcgccggagt cgatgcaagc
    48121 gatcttcgag cctgttatga taccaggcca cgatcaggaa caggacgaag ccggacgcga
    48181 ggaccagatt gccgaagagc gtccagccta acttatgcgg aatgatgctg ccgaggaggg
    48241 tcgcggcaaa aatcgtgaga cgagccgtgg taaaccgcgc actgacgcgg cgaccattcg
    48301 tttccagatg aaccgctcgc cggagtgcat ggacgagatg ctgctcacgg gatggcttca
    48361 attctgctga cgtaactggg ttcacaataa tgctgacgat actgggagtc tgagatgacg
    48421 tcaacctcca aggtagatca atcttcttcc tccgattccg ccgcggtgca aacaggggat
    48481 tggatacaag ttgatgtgcg tacctcgctg gatgccggtg agctattggg aatgctcaac
    48541 gatcctgacg tgacgggggc ctggcaagag ggtgacgagg tgcatctgta ttggcccgcg
    48601 ggccgttgcg ggccggatac ctggcagggg ctgaaacgtg cgctgactca acttggccat
    48661 ccggacccgg atcacgcggt gaccatcaat catttggcca atcaggattg gaatgcacaa
    48721 tggtcccggc tggtggaacc catcaagatc gggcgggtcc tgatcaggcc gagctggaga
    48781 accgtctcgc tcaattccga tgagatcgaa ttgatcatcg atcctaagca ggcattcggg
    48841 accggccatc atgcgacgac acagctgttg attgaatggc tgcaacaggc tgtgcgaccg
    48901 gacgatcgcg tgttggatgt ggggaccggg agcggcatcc tggcgatggt ggccttacgg
    48961 ctcggagcca cggaggcgct cggggtggat ttcgatgccg tcgcggtgga ctgtgctcgc
    49021 gagtacgcgc aggtgaacgg gtttgacacg cgtctcacgc tggccgtggg acaggctcag
    49081 gccccgcaag cccatgatgc gttcgatccc accctggtgc ttgccaatct ggatcggcag
    49141 acgctcctga catccgcaga tagcctctgc gcgtatgccg tccgcggcgc ccgcctcttc
    49201 ttgtcgggac tgttggccga gcagcggtcg gaaattgaag cggcctatgc cactcgcgga
    49261 gtctatatga aggccgttcg ggagcgagac ggatggctcg cattggaggc gaccgcgatg
    49321 gaaacgtgcg agggcggtct ctgttcctga tgcggccatg acgcgatcgg cgactccata
    49381 tgtgcatcag atcagcgttt ccgatggtgg agtgccgaag ctggccgtcc cctcggcgag
    49441 gatcaccatc gacggactgg ccggcgaccg gcagcgcaat cgaaagtatc acggaggaaa
    49501 agatcgggcc gtctgcctgt tctcgctgga acgcatcgaa gcgttacgcg ctgaggggca
    49561 tacgattggg ccgggctcgt ccggtgaaaa cctcacggta gccggtctcg attggcagca
    49621 catggcgccg ggtgaccaac tcaccatcgg cgagcatgtc cggcttgaat tgaccagtta
    49681 caccacgccg tgccgcttga atgccccttg gttcaaggat gaagattttc gtcgaatcgc
    49741 gcaggaggtg catcccggat ggagccgcct ctacgcgcga gtgctcaatg agggcgtggt
    49801 gaagccgggg gatgttgtgg tagtcgacgc ctgtcctggg gagcggtgag tgatcttgat
    49861 ccacatgtag gaacttatga tgaagcgagt acttgaaccg gaattgatgg atgatgtggc
    49921 gcaagctctc gcctatgcca aggcggattt tgccgaggag aaccagagct ttgtcgaccg
    49981 ctttcgtgaa tattttcccg cctgggacgg cggccatgtg gttgatctcg gatgcgggcc
    50041 tggcgatatc ccgattcggt ttctccgcgc ctatccacat gcgcgagtga ccgccgtcga
    50101 tgcgagccgt cccatgctcg atctggcggc ggacgccatt gccggagcag ggttgacgga
    50161 tcacatcacc cttcgttgcg agcggtttca gtcgctcgtg ttgccggaac aggccgacgc
    50221 gctcctgtcc aacagccttc tgcaccatgt gccgaacccg cttcagttct ggtttcggct
    50281 gaagcagctg gcaaagccgg gcgcgtgcat tttggtcatg gatctattgc ggcctgactc
    50341 tcctgaagct gcccaggcgt tggtagaaca gtatgctgcc gatgaagatc cgattctgaa
    50401 gcgggatttt tatcactcat tgctcgcggc cttcaccgaa gacgacgtgg cggcgcaact
    50461 ggctgaaatg aatttatcgc ggctcctcat cgatgtgcct gacgatcgtc actgggtagt
    50521 gggcggaatc atcccctgac tgcctgttcg acacaagagc tggccgcgag ggctcacgtg
    50581 cacgcctcta tgagcgctgt ttgtccagcc agatccatct tcattgcgac cctgcgctgc
    50641 gtgcactccg cggtgctcgc ctgcctgttg gccatagtca tgttgtatcc gacagaatct
    50701 gctgccgggc ttcttgaaca gttgcgtaag ccgcgcccga gttcggaact gcaggtccac
    50761 tccgacgtac ccatgatcat gatccccgaa ggagtcttcc ggatgggtgc cgatggaacg
    50821 gacgccttgg aggatgagaa gccacaacat gacgtgtggc tcagccggtt tgagatcgac
    50881 cgtcatgagg tgacgaccgg acactacgcc gaatttctcg cctccacgaa gcgaccggct
    50941 ccctggcaat gggagtcact cgatctgtca cagcacggcg accgtccggt cataggcgtc
    51001 agctggttcg atgcggacgc ctattgtcga tggcgaggca cacgtcttcc gacggaagcg
    51061 gagtgggaaa aagcggcacg agggaccgat ggacggttat ttccctgggg caatcacagc
    51121 ccgcaagagg aaacggcgaa tttcggatta ggggccaggt tcagctacag ccaggtgctg
    51181 gctcccgtag agcgctacga acaagggaga agccccttcg gcctgtatca gatggcagga
    51241 aatgccgggg aatgggtggc ggattggtac ggggcacact attacgagaa cggtccgcgt
    51301 cagaaccctt ccggacctga tgccggagca tttcgcgtgg tgaggggagg atcctggtcc
    51361 gacctgccga aatatctcct gacctatggc cggttcaagt tgcccccgga gactcgcaac
    51421 agttacaccg gatttcgatg tgccagagac ggtggggtgc tagcttcgcc gtgagccggg
    51481 cgcccatgcg ccccagctca cggtttctcg ttcggtctag aacagactct tactgacttc
    51541 cgcagatttc gcactctcgt ttcccgactt gtcaaaagcc gagacagcaa agaagtaggt
    51601 ggctccgagg ggcaggttcg gtacggtgaa gctggtgccg tttgtcacct cgaatggtcc
    51661 cacgactccg taacttcctg atttcgttcc cacatagatg cggtagccgg ccaggtcaga
    51721 ctccgtattg gcgttccagc ttaccgtcgc gctggcagtc ttgggcgcgg tgcttgaggc
    51781 ggacgacgtc ggggtcggcg tcgtggtcac cgggaccggt gacggagggc tcggaggagg
    51841 cgggggcgga gaggccaccg ttggttgtgt cggtgtcgtc ggggtgctgg aggctgtttg
    51901 acccgcggcg accgtaaagg tcactggaat gcggagcgta ttggcgaagt tattgggacc
    51961 agattcaacg atgtacacga ctcccgaata ggtgcctgcc gccaggccag ccgtttgagc
    52021 ggtgatgacc agttgatcgc tttcggtcgt aatggtctgc gtgctgccat agggcggatt
    52081 catccagatc cacccctggt tcgtgctgag atagtacgcg tgttggtcgg ttccggtttt
    52141 gcggagcgtc aaggtgccga cgttattggc gctcgtcagg ctcaaggagg acggggtcac
    52201 ctgaatcggc cccgtggcaa ccgtgactgg tgctggcgca ggtggggccg gagctggagg
    52261 cggtggtacg accgcgggtg ccggcggcgg gggcggtgcc aggacgactg gtttcggcgt
    52321 cggaacgatg gccgggggaa ctgccgcggg gggcggaggc ggcggtgcca cggccgatgc
    52381 cgtcaccgtg agggtgaccg ggatgcggag catgttctgg aagttattcg gtccggattg
    52441 gacgacatac acgacggccg agtaggttcc ggccgcaaga ttggccgttt gggccgtgat
    52501 caccagctga tcggtttccg tcgtaatggt ttgcgtgctg ccatagggcg gattcatcca
    52561 gacccacccc tggttcgtgc tgagataata gctgtgttga tcggtaccgc ccttccgcag
    52621 tgagagggtt ccaacggcgg tttgcccctt gacggcactc aacgccaacg cagccgggct
    52681 cgacgtgatg gccgttccgg ttaccaccgg cgccacgaca ggagtcggag ccggaggagg
    52741 aggcacggcg accgggctgg gcgtcgggat aatggccggn nnnnnnnnnn nnnnnnnnnn
    52801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    52861 nnnnnnnnnn nnnnnnnnna gattggccgt ttgggccgtg atcaccagct gatcggtttc
    52921 cgtcgtaatg gtttgcgtgc tgccataggg cgggttcatc cagacccacc cctggttcgt
    52981 gctgagataa taactatgtt gatccgtgct ggattttttc aggttgaggg tgccgaccgc
    53041 cgtttgtccc ttctgggcgc tgaggacgag ggcagttgga ctggtaaata tgggagccgt
    53101 gccgctgttc tgggccatga ttgccaccgc gtcagtcgat gactgctgca gcaggctgag
    53161 cgtggtgtct gcggcagcgg cggttggggg caaggctgtt gaacacagcg cggaagataa
    53221 gcaaccggca acaccgataa ccaacacact cttgggcgtc ttcatgatag ttcctccgaa
    53281 attccagacg ctggatgagc aaccagcatg ccagctcgaa aatttgtgcg aacgtcagaa
    53341 agccgcgtca caagccattt tgcagagaat ggcgctgtgc gatagtgttc gttatttttt
    53401 gctgtgcaag ttggagcagt gggtgcagga aaatgccttc gatcttgttc gcgggagcgt
    53461 ctgtgaagca gacggccagg cgactcgtcc acttcattgt gtagtgctcg gggaatgaga
    53521 gcgggtcacg ccgcgggagg cgccgcaatc caatccgtga gcaatgtcaa catcagctga
    53581 aagtcggagg ggtttgtaaa ccggtgatca gcaccgggaa ggatttccag gtgcttgggg
    53641 cctgccagcg atgcatacag tcgttggctt tggtgcagag gcacgtattc gtcatgatcc
    53701 ccttgaacga tcagggttgg tgttgtaatg gcacgagccg gttcgtaggc gatgcggttg
    53761 agacaatcct ggtagaaggc atagtcgagg gggaggcggg aaggacctcc atgaagatcc
    53821 gggattgtgt ttgtacgttg ccactcctgc agcccatctt cccccagctc taagcgcagc
    53881 tcttctccga aatcaacgac cgggcattta agtgcgaggc aggcgagagg gggaacatcc
    53941 gccggcgcgg agttgtgtag atcggtccat gcagaggccg cgagcagtga gacaaggccg
    54001 ccgaaactcg agcccacgag tgcgagtcgc cgataacccc gggtcatggc ataggttaat
    54061 gcagcgcgtg cctgagtcac cccgatgctg gtcgtcagct tggcgaaggg cccatcgctc
    54121 tcaccatggc cgaaacagtc gaatcgcaac gtggcgatgc cgcgtgtttt caagagttca
    54181 gccagggctt ggttactcgt gctgttcttg tgagataaaa agccatggca gagcaccgcg
    54241 acagagtcgg tgctctgtgg tggtgtggcc agcaccgccg caatccggtg gccggcctga
    54301 tcgtgaaatg aaagcgcttc ttccatagtg gtgaagtgat ggcggttatt tcgccgctcc
    54361 ttcatgggac ggttgagcgc cggtcgcctg tgcgagtttg acggtgacca tggtgaagtg
    54421 aaacagcaac agtcccaagc ttatccaggt gctcgtttgc agccagggcg aggacagggg
    54481 aagattccag gtcaatgagg cgacgagcac caaccccaaa gatccaaaac ttacggtcgc
    54541 aacctgggcc gcccgccaac ggttcatgat gcgttcaagg tcttcgcgat aggttccccg
    54601 ctttttctga ttcgcgatcc gcgatgtggc cagcagcgac ggcaacgtga aggagagtcc
    54661 gccgaccatg ctttgcagga gaaaaccgag gaaggcggtg tgggtgtagg ccaccagatg
    54721 cagcgtccca tagggcatgg ccggcggtgt cgataacacg ttgatcccga atgccaagcc
    54781 gatgaaggtc ataatcagca ggaagctcaa tgccgtgagg agatggtccg tggcggcaga
    54841 gccgggttga ccggcctgac tccatgtgcg cagcaggttg atgcagcaca gcgccagcgc
    54901 gatcaggagc acggttcccg ccgccaactg gatatgaacg gaggacaaca cgaatcccgt
    54961 gatcagcccg gcggcggagg ccggcagaag cagcatgacc gcctggccca tcccggggct
    55021 gtgcagcggg cgatgtacca tcatcgggag cgtcagttgg atggttccga cggctccgag
    55081 tatgaggaac agcaaaaggc ctgcatggag atggcccaga cggagaatcc cgtgccaggt
    55141 gggataccag gcgtcggcga gcaggcctcc cagcaccatg ctgccgaaca ggccggcgaa
    55201 gctgatgccg aaatagacat gtaccaatgg tgtccatcct gagcaggtcc ggagcgcagt
    55261 cagcatatgg cgggccatgg ggacaaagag cgcggccagc aggaatcctg cggccagggt
    55321 caaggtggga ttccggaacc aggctccggc agcaaaccca acggccgtaa gattcatgct
    55381 gagaaggagc aagcggtgtg tcgttcgttt cccctcatgg ccggacagat catgtgccgc
    55441 gatcgccagg ccggtcatca attggagaac cccgccgatc agcactccgt ggacgtgtaa
    55501 aaggcgaagg ccggagggaa gcgggcctcc ccgtacgatg cccccaaatg cggcgagtcc
    55561 caacagtgcg gtcagcagca cccagcccca tccggcgagc agaaacgaca gtggcgatcc
    55621 aagccctcga ctcatagcct ctaaccctga ttcaagaagt aaaaatgatt ggtcggtccc
    55681 ttgccatgcc caatggccag gccatgtctg atggcttcag tgagataggt cttggcggtt
    55741 tggacggcat cagagacact tttgcctcgg gcgagatgcg ccgcgatggc agaggcaaag
    55801 gtacatccgg tgccgtgcgt atggggcgtg tcgatgtatt cacccttgaa gatgttgaag
    55861 aaccgtccgt catagagcag atcggtcccg cgctcttttt gcagatggcc ccctttgatg
    55921 aggacattgg cgcaaccgaa ctgatgaatg actttggcgg cacggcgggc atcggccaac
    55981 gaagtgatct cgatccccga aagctgctgc gcttcatgca cgttcggcgt gacgagcaaa
    56041 gccagcggaa agagcgcgct cttgagggta tcgatggcat ccggcttcaa cagcgcctgc
    56101 ccgcctttcg caatcatgac cgggtccacc acgagatgtt tcacctgctg aggcttcagg
    56161 agtttcacga ccgtccggat aatctctgcg gaggagagca tgccggtctt caccgccgcc
    56221 acgtcaaagt cgtcgaagat ggcatcgagt tgagcggcga tgattgatgt gggcagttcg
    56281 aacacgtcgg cgacttcctc tgtgttctgt gcggtgatgg ccgtgatgac cgacatggca
    56341 tagacgccat tggcggacat ggccttcaga tctgcctgaa tgcctgcgcc
//
LOCUS       DAOQDS010000021        55602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_179_length_55602_cov_20.402560, whole genome shotgun sequence.
ACCESSION   DAOQDS010000021 DAOQDS010000000
VERSION     DAOQDS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 55602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55602
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179_length_55602_cov_20.402560"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1774)
                     /gene="thiC"
                     /locus_tag="PK589_06950"
     CDS             complement(<1..1774)
                     /gene="thiC"
                     /locus_tag="PK589_06950"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNJ19375.1"
                     /translation="MSEAHTQNGSSNGHGAKPSTARLTTTPFAASRKVHVEGSQPGVR
                     VPMREISLTPTHSTNGNGGTPNQPVTVYDTSGPYTDPNVTIDVRAGLPPLRRMWIEAR
                     QDTEELPQVTSQYGRQRAADPKLADLRFTHIRKPLRAKAGRNVSQMHYAKKGIITPEM
                     EFIAIRENQARARAKDLMASSNGHGGGVSQHPGQAWGARIPSLITPEFVRDEVARGRA
                     IIPNNINHPESEPMIIGRNFLVKINSNIGNSAVASSIEEEVEKMIWSIRWGADTVMDL
                     STGKNIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEDLTWEIFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTAKRMTGIVSRGGSIHAKWCLSHHQENFAYTHFEEICEIMKAY
                     DVAFSLGDGLRPGSIADANDEAQFAELETLGELTKIAWNHDVQVMIEGPGHVPMHMIQ
                     ENMEKQLKECQEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGCAMLCYVTPKEHL
                     GLPTKEDVKVGVVTYKIAAHAADLAKGHPGAQARDNALSKARFEFRWEDQFNLALDPE
                     TARSYHDATLPDNAAKVAHFCSMCG"
     regulatory      complement(1866..1968)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(2053..2610)
                     /locus_tag="PK589_06955"
     CDS             complement(2053..2610)
                     /locus_tag="PK589_06955"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HNJ19376.1"
                     /translation="MKKRMTPTLGRFVYLVACGLLSNPVLLWPTSGAQAESFNAKPGA
                     WEMTFTTQSSGMLVPPDVLAKMPPEQRAKVEQSMQARSGKPKTHSVKTCLTKEDLDQN
                     RIIKENDDDEPGCKTSVVSKSSAKLVLERTCPAPQASTSRMSVEAQTAELLVGSMDTD
                     RPRAGKVHIDFKSRWLGANCAGIAE"
     gene            2780..3376
                     /locus_tag="PK589_06960"
     CDS             2780..3376
                     /locus_tag="PK589_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNJ19377.1"
                     /translation="MNGRNFWIGLVVGASAMGILGQWGPAVAGKDAIQPTCIPAAAVA
                     EYLHSVIQADRTFYTTDVVERMQMRGIAFAAENWRETSRLPLPAQYLLETGRLVAAGR
                     SGVQYRLISNWAINAKNRPMTDFERAGLTEILVNPDQPYTAVTTEGGAPVFQAIYADK
                     AASQSCVGCHNAHPNSPKKDFKPQDVMGGILLTIPLPQ"
     gene            3601..4728
                     /gene="nadA"
                     /locus_tag="PK589_06965"
     CDS             3601..4728
                     /gene="nadA"
                     /locus_tag="PK589_06965"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNJ19378.1"
                     /translation="MDSTERLQVKTVTTLPRPITDYQSLSAEELFERTRAAKRALGDR
                     VMVLGHNYQRDEVIQHADFRGDSLILAQVAEQRSDRPYVVFCGVHFMAETADVLSRSN
                     QTVILPDMAAGCSMADMAAIEQVEQCWDALGRVVPVEETVMPAVYVNSAAILKAFCGE
                     HGGLTCTSSNARKVIEWSWARREKILFFPDEHLGRNTANKMGIPREQMIVWDPYMPRG
                     GNSVEAIQRAKLILWKGHCSVHQMFQPSHIDYVRKKHPEVKVIVHPECHEDVVNKADL
                     VGSTEYIIRTITAAPAGTTWAVGTELNLVNRLKHEQADKKIFFLSSTVCQCATMYRID
                     APHLCWALENLADGHVVNRIVVPDDEKQLAKVALDRMMALS"
     gene            4991..7810
                     /gene="ileS"
                     /locus_tag="PK589_06970"
     CDS             4991..7810
                     /gene="ileS"
                     /locus_tag="PK589_06970"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNJ19379.1"
                     /translation="MDYKSTLNLPKTDFPMKANLPQREPEMLARWAQERLYERIQESR
                     QGREQYILHDGPPYANGRIHIGHALNKILKDIIVKSKTMSGYQVPYVPGWDCHGLPIE
                     HQVLKELGDKKRDLDALAIRKLCQEYAAKYVAIQREEFQRLGVLGEWQQPYLTLNPAY
                     EGTIIREFGRFVERGGVYKGLKPVLWCTQDQTALAEAEVEYDDHTSPSVYVKFPLVSP
                     PSVLSKRFGPVSFPVEATRASVLIWTTTPWTLPANQAVCLHADIDYAFVLVGDELLVM
                     AEKLVESVAKACNIPTYTIVAVKKGREGFEGLEAQRPLTTGLSPLLLGDFVTLEQGTG
                     CVHIAPGHGMEDYLLVLDHNAKASVGERLEILAPVDNGGKFTEAVPEFAGQHVFKANP
                     KIVERLKGNGRLLGHGSLNHSYPHCWRCKQPVIFRATEQWFVSMETNDLRKEALAEIE
                     RVRWIPAYGRDRINGMIENRPDWCLSRQRVWGTPIPGFTCLTCGKVLAHPRVIDHVAD
                     LIGQHGTDYWFANQAGALLPAGTQCEGCGGTDFEKERDILDVWFESGVSYAAVLKPRQ
                     WYPADLYLEGSDQHRGWFHSALLSGVITDHRAPYKAVLTHGFVLDGAGRKMSKSAGNV
                     VAPQDVIKQSGAEILRLWVSAQDYREDLRISQEILNHLIEAYRKIRNTCRFLLSNLYD
                     FDPAQHRVPFEQLPELDRWALMRLSDLIPRVRKGYDEFEFHTIFHALNNFCSVDLSAV
                     YLDILKDRLYTFRKDSPLRRGSQTVLFDIVVALTKLMAPVLSFTAEEIWRTLPESVRT
                     EAKADSVHIAMFPEVDPRWADPMLAQRWEQVLEVRSAVQAALEVKRREKVIGAPLEAR
                     VVIEANSERYEFLKRYEQELSSFFIVSDVVLTQAGHLAESPGYSVTVDKATGAKCERC
                     WNYRSAVGTSADHPTLCDRCIEAVR"
     gene            7807..8328
                     /gene="lspA"
                     /locus_tag="PK589_06975"
     CDS             7807..8328
                     /gene="lspA"
                     /locus_tag="PK589_06975"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNJ19380.1"
                     /translation="MSQSIRYLLLASLSLAIVVLDQVTKVYVMETMRLHESIPVIANL
                     FSITYIRNPGAAFGFLSSSSSSFRFVFFGLTSIFAVGLLGMIMVRMPKEDWMGRLSVA
                     GILGGAIGNLLDRLRYGEVIDFLDFYVNGYHWPAFNVADSAITVGVVFLILHFATEKE
                     PADQPVASEQPPS"
     gene            complement(8347..8613)
                     /locus_tag="PK589_06980"
     CDS             complement(8347..8613)
                     /locus_tag="PK589_06980"
                     /inference="COORDINATES: protein motif:HMM:NF021169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HNJ19381.1"
                     /translation="MASNMDSVHVYDTWVKGKNGKLHFDVMTTSQEKALALAKQYLVG
                     IGEPQAVITLKECQFCHSEPLAMFSVKQQRQFREQGGFIITLPV"
     gene            complement(8642..9517)
                     /locus_tag="PK589_06985"
     CDS             complement(8642..9517)
                     /locus_tag="PK589_06985"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNJ19382.1"
                     /translation="MMNWGPELAVILGIIEGLTEFLPVSSTGHLILVGHALGFTGDIA
                     SNVEISIQLGSILAIIAYERAKLASLASHALREQQEFRSLIATRGTKSWATTLQESIN
                     AQPNLWFVIGLGLAFLPAALVGFVAHKSIKAYLFSPTTVAISLIVGGVIILVVERMQS
                     RVRVKELLQVTPRSALLVGLAQCASLIPGMSRSGSTIVGGLLAGLDRRVATEYSFFLA
                     LPTMIIATIYQMLKSQASFSQDDYVALGIGLVVSFVVAWAVIAAFLTFVQRHSLSVFA
                     YYRMVLGVIVLLVVR"
     gene            9769..10482
                     /locus_tag="PK589_06990"
     CDS             9769..10482
                     /locus_tag="PK589_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNJ19383.1"
                     /translation="MRHVWVAGLALMSAMAVSGCVSDKKYHEALADADATRGELERAR
                     AQKNALEQQVKTLKDLNAKFGSESQIARDELQRIQHGRDKERDTIDVRTKELEDKVKQ
                     LSAQNRSVKQEYEEARRHNEQLKSLVARYQKEMKDRSRSLSSGMESPALTAPLGGGGA
                     SSKAAPAPAPFEAPAAPLLNINKAPSSDLVLTLGITQDVADRIVSNRPYRVKGELVAK
                     NVVTKDVFDDIKDRITVSP"
     gene            10671..11954
                     /locus_tag="PK589_06995"
     CDS             10671..11954
                     /locus_tag="PK589_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ19384.1"
                     /translation="MRRVSIIVGVLALGLAIAGYVFFNGERKVPLRFRTVPVERGSVV
                     SLVTATGTINPITTVQVGSQVSGMIESLHADFNSKVKANQVVARIDPFPYQARRDQAV
                     ASLANAKAAFEKARIDLAQRRRELDRAKSLIGQQFISQNEVDVALTASEGAVAQLKVT
                     EAAVKQAEAMLQAAELDLKYTVIRSPVDGVVISRLVEVGQRISASFSIPTLFLIAEDV
                     TKMQVDTNVSEADIGGIVDGKAASFTVDAYPGEAFQGRVRQVRNAPINIQNVVTYDVV
                     VEFENPEFRLKPGMTANVSIVVNKRENVLKVPNAALRFVPPKAVRDEKGEAGPRRDGE
                     RAGGRPTIASGSPESGNRQWGVWRLDGAGELERVPVEMGISDRSYVEITAAGIQEGDQ
                     VVTGIESPRGERKSGELPPGFGGGQQRGGRRDRGM"
     gene            11957..12709
                     /locus_tag="PK589_07000"
     CDS             11957..12709
                     /locus_tag="PK589_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ19385.1"
                     /translation="MVETVACDPVCEGTMAPLIVCEDLWKIYRIGDVEVQALRGINLT
                     IDRGEFVAVMGTSGSGKSTLMNLLGCLDKPSRGRYRLDGLDVSTANPDLLADLRNRQI
                     GFVFQNFNLIPRTSALENAQLPLFYRGVSIKEQRRQAAAALQRVGLAGREQHYPAQLS
                     GGQQQRVAIARALVGAPSILFADEPTGNLDTASSREIMDILEHLNREDGITIILVTHE
                     PDIAAYASRELVMKDGQIVQDVRREPHLSAVT"
     gene            12716..13951
                     /locus_tag="PK589_07005"
     CDS             12716..13951
                     /locus_tag="PK589_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ19386.1"
                     /translation="MPAFVLLTVMTALRILSRNRLRAGLTMLGIVIGVGAVIAMVSIG
                     QGARAAVQAQVASMGTNVIVIMPGSTTVSGVRGAQGGAVTLTINDAAELKKRSPLLSD
                     TGWAKRDVMQIVNGNRNWNGPVNGVSPSYLTIRDWSFTSGGPFTQTDMDSAARVALIG
                     QTTLENLFDPGEEAMGATIRIKNVPFQVVGVLAPKGQSAQGTDQDDVIFIPFTTAERR
                     VFGSQFLGSVGALFASTEQSGDLPEAVEHIRETLRLRHRLEPDQADDFTIRTQVDIGK
                     VQESTSQTLTVMLFAIASVSLLVGGIGIMNILLVSVTERTKEIGVRMAVGAKRIHILT
                     QFLIEAMTLSLFGGLIGVIVGIVGARLTTVIAGWPTMISVDAVLTAFLFSLAIGLFFG
                     LYPANKAARLNPIEALRYE"
     gene            complement(14024..15262)
                     /locus_tag="PK589_07010"
     CDS             complement(14024..15262)
                     /locus_tag="PK589_07010"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNJ19387.1"
                     /translation="MTYRPITIDALRIGMHVAKIDVAWFRSPFMRHSFLIRTDEQIEK
                     LRRAGVKQVSIDPSRGLDLQDPMAPSQAPLPVAAQPATSQMGKSVEIRSLATMTQELL
                     AARSARAKLEESVHSAFSRIAKTGVVDPEEASHAVHAISAVAQALNTSALFMVFSQGR
                     EANAPLSQHALATCSFSMILAHAADYNLMAIQELATGALLHDIGLLQVPPAILRRVHD
                     TSTTVSEQNRLTYEAHARSGAILLERRGGFTKPVEQIVAEHHAYLNGSGFPAETGGAF
                     TSDMTRIVMVTDRYDELMTGFGGASPLTPHQSLQRLYQEGQEGRYESRLISLFVKVMG
                     IYPVYSYVQLTTGERAIVSVINSGKLHQPIVTITHDPSGEPYIVPLVIDLANQDEEAP
                     PRGVRSVLGTIPEEFQRAYH"
     gene            complement(15318..15881)
                     /locus_tag="PK589_07015"
     CDS             complement(15318..15881)
                     /locus_tag="PK589_07015"
                     /inference="COORDINATES: protein motif:HMM:NF017663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HNJ19388.1"
                     /translation="MERAPLIPTQSNRNRHALSFPTPAGTGLSVTIDSITLTLPVPPS
                     INHQYATVNGRRLLSAKGRTYKDFVGQQIVVALAQSPHRATLRQTLQQASLSLSIHFF
                     FASALRRDTDGGLKIAQDALCEGLGLNDNRVVETHLYKHQDRDNPRMEIRLSVAPSTA
                     PDPFFPASLKAQVAVIPKPPIDNGQDR"
     gene            complement(15974..16414)
                     /locus_tag="PK589_07020"
     CDS             complement(15974..16414)
                     /locus_tag="PK589_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19389.1"
                     /translation="MFAGEHLCKVDEKGRFLIPSPLRERFQAEGNQVMFLKNTEQTLW
                     MYSAKEWEQVLERTRATLDEDQTRLFMHHVMAQAGASDIDKAGRVLIPSRLRKLVPMD
                     EDQELVLVGMYHRLEIWGPSEWRRYLTRNEDRAEQDMAKIQNLL"
     gene            complement(16492..16836)
                     /locus_tag="PK589_07025"
     CDS             complement(16492..16836)
                     /locus_tag="PK589_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ19390.1"
                     /translation="MPIYEYRCRQCGKRTTRLILNANSVPQQTCAHCQSADMERLLSR
                     FASPKSEEARLEALSDPSNLSGLDENDPQSMARFMKKMGQEMGEDLGDDLDAAMEDGQ
                     APASGIDGTDFD"
     gene            complement(16840..17631)
                     /locus_tag="PK589_07030"
     CDS             complement(16840..17631)
                     /locus_tag="PK589_07030"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNJ19391.1"
                     /translation="MDFLRQASRLLLPMDCTTCAQPLADDPVPFFCRRCWELIRPLRG
                     PSCPRCHRPFASPSALTYSPTHECQDCRTREPYYSQVWAPYAYCSPLQDAIALFKYRG
                     KVALADALGVLLLQAIPDRLDVDVLMPIPLHPTRLRQREFNQSLLIADRIGSARRLPV
                     SYRNLIRTVDTAPQTSLPRAARLQNLRKAFAIRQPKEITGARILLVDDVFTTGTTASE
                     CARVLLTAGAKQVAVLALARSADAGMVPDTWLPPSTLNQTEFQRA"
     gene            17869..18543
                     /locus_tag="PK589_07035"
     CDS             17869..18543
                     /locus_tag="PK589_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ19392.1"
                     /translation="MSVHNLSKEYRRGGTVLCALQGVTLEVKAGEFCAFVGPSGCGKS
                     TLLNLIGGLDQPTSGTIMIEGRAVTDASATEWTRLRRELIGIVFQAFHLIPGLTVAEN
                     VALPMLLKGEQGRAVTDRIDEVLQAVGMEARRSHRPSELSGGEQQRVAIARAIVHRPR
                     LILADEPTGNLDSKQGADIVNLFRTLPQRFGHSVLLVTHSDSAAEAADYVWHMRDGRL
                     VNRIAH"
     gene            18617..19543
                     /locus_tag="PK589_07040"
     CDS             18617..19543
                     /locus_tag="PK589_07040"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HNJ19393.1"
                     /translation="MSLSMTIAGVTFPSCLMNASGALCMTREELLALGRSRAGAIVTK
                     SMTVEPRGGNPEPRYYGFPGGSINSMGLPNLGYLAYADLIPELKQFGKPVIASIAGLC
                     EDDFLTMARVINHAKPDLIEVNLSCPNIPGKPQIAYDPVDSERLLKRVRPLITVPMGV
                     KLPPYFDPAHHAVMAEVIGRCGVDYLNLINSVGNGLVIDPKRETPVIKPKGGFGGLGG
                     SLIKPVALANVRAFWKLLGGRIPIIGTGGVVQGLDAFEHVLCGASAVQVGTVLVEEGV
                     GVFERLERELEAELSSRGARRVEDYRGRLKEL"
     gene            complement(19586..19921)
                     /locus_tag="PK589_07045"
     CDS             complement(19586..19921)
                     /locus_tag="PK589_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ19394.1"
                     /translation="MALRKRPQAKKSSKTTKAAPAAKKRKPVKLAKASPGRTRRSDGL
                     TPRQKEILKLVSQGNTNRDIARRLDISVRTVEVHRFNLMRRLKVRNVAQLLRQALQQG
                     FLPKNFAFR"
     gene            20020..22674
                     /locus_tag="PK589_07050"
     CDS             20020..22674
                     /locus_tag="PK589_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ19395.1"
                     /translation="MSLFHSSLLRVCASLGWTHLSTHPGRTALTMFGVGLGVAATIAV
                     QTANVEVLRSFEESVLTVAGPVTLEVSAGETGLDERLISTVRTVPGVLSARPVLEVGV
                     RVTDGRSPRQSFSILGLDLLEELSPEGGRVVSPANRGEGAGERMPLEDLLARNGLLIG
                     HALASELSLALRAQLALQSGDQHVSVSIIGVMGRRPGGPSPWDRMAVMDIAAVQRTFG
                     LIGRLDRIDVVTTPTSSVEQVAEAIGRVLPPATTVRRPIQRSQQVESMVGAFQLNLSV
                     LSMVGLLVGIFLIYNTVSFTVAQRRREVGILRAIGMSEPMVVAVFLAEAGVFGVVGGL
                     LGGMLGLLLGDVLVALVGRTIHDLYTPLSHSTGTVGFPVGSGRLLVEAVLIGSGVSVL
                     GALGPSVDAGRTIVVAALAPGEYDVAQRVRAASLGVAGGVLLLIALGCALAGPVGGVP
                     IFGYVATFCLLAGLSCLVPILLQSLCRTRELTTLSQAPTLGGAIRHIAREQTSRGMGR
                     NAVTVSAFLVGVAIMVGVLVMIRSFRDTVEIWIDQTVMADFIVAPAGWPHAVRSGSSS
                     PVLPGAWRARLANAPLVSAVDMYRDLRIDLQGRPVSLVSRDLALHAARSRYLFVTGES
                     APILARAAAGEGAILSEVVANHLALGKGDRLSIVTPKGEQSLEVLGVFYDYATDGGKI
                     VIDRSLYQQWWPDDGVTVFPVYVESGADMGKARMAILGTLAQASQGNLMPTVLSNAEL
                     RQEILRIFDRTFTLTYVLEAIAVIIATLGIINTLVTSVVERRRELATLQALGSSRGQV
                     TALILWEAGYLGLLGTGMGLVGGGALALILIRVINRQSFGWTIQISWPIGLMAEVAAL
                     ALVASLVAGFWPARWAARQPLVEGLRYE"
     gene            complement(22665..23696)
                     /gene="bioB"
                     /locus_tag="PK589_07055"
     CDS             complement(22665..23696)
                     /gene="bioB"
                     /locus_tag="PK589_07055"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNJ19396.1"
                     /translation="MTDFTILADKALRDELLTREECLTVLHTPDTNLLELLQAAFKVR
                     ERYFGKTVRLQMLLNAKSGACQEDCGYCSQSSVSTAPIERYGLLPQDQMVTGARRAAA
                     AKAQRYCIVISGRSPLDREIADIASAVRSIKQEVPIQICCSLGLLNERQAKDLKAAGV
                     DRINHNLNTSEAYHTEICSTHTFQDRLATIRHARTAGLEICSGGIVGMGEGDEDLIDL
                     ALALREVKPDSIPLNMLHPASGTPLEHCTPLTPQRCLKALCLFRFLHPRTELRIAGGR
                     EHNLRSLQPLALYPADSVFVNGYLTTPGQPAAEVWRMIEDLGFDIQVDALPTQGATSS
                     AEPAVGLHS"
     gene            complement(23797..25197)
                     /locus_tag="PK589_07060"
     CDS             complement(23797..25197)
                     /locus_tag="PK589_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ19397.1"
                     /translation="MFRLLSTIFLVSVGIFLYSYFRELNPGTITVRTSPDAIFELSPV
                     SLVLFSMALGATLVALIVTIKETSHVFMNWRTNRLVRRKEKVDALHRDGTHAFMSKRT
                     ADAVSLFERALVIDPNRTDSLLWLGNIYRSESNFTEAIRLHQQAYRIEERNVEVLLEL
                     AKDLEGARRYEEALQTLQNILRIEPDNLMALIRKRDLQIRLEKWSDALEIQHRLVKAN
                     LPESERRAEANLLLGCMYEVGRQLLERGHPDKARRYFRGAIKKDRTFLPAYIGIGEIL
                     VREGKIKNAVEILKKIYAKTRSVIILHRLEELFLELGEPDEIIRVYQEALQQDPHNAV
                     IQFYLGKLYYRLEMVDEAYDVLSTLDGTQEQLVDYHKIMANLFLRKQHMDEAVGELKK
                     ALGFKKRVVVPYQCTQCHHELAEWSGRCRRCGRWNTYVALPWRDSTKSGADQDGQAAR
                     LPAVPYQGIASPFETV"
     gene            complement(25199..25621)
                     /gene="rsfS"
                     /locus_tag="PK589_07065"
     CDS             complement(25199..25621)
                     /gene="rsfS"
                     /locus_tag="PK589_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNJ19398.1"
                     /translation="MSVSETKAKALAVAAAILDKKATDVLILHIAKLTSIADYLVIGS
                     GESERQARAIADHVGDVLKAQGHPPLGIEGASSAKWVVMDFGDVIVHIFQKDIREHYA
                     LERLWGDAGQVKLPEERAVKVTPPARTKARPARTRKRV"
     gene            complement(25608..>25917)
                     /locus_tag="PK589_07070"
     CDS             complement(25608..>25917)
                     /locus_tag="PK589_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19399.1"
                     /translation="LCHFVVISRPATTFLTVASLPLFPEVPKEELLALDTGRLERADV
                     PLKTGRTVTFLRLPPCESSASEIRARVADGRALANLLPPLVESYILREGLYREDSERF
                     "
     assembly_gap    25918..26017
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26018..26443)
                     /gene="nadD"
                     /locus_tag="PK589_07075"
     CDS             complement(<26018..26443)
                     /gene="nadD"
                     /locus_tag="PK589_07075"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNJ19400.1"
                     /translation="MRLGVLGGSFNPIHRCHLSIAQSARRLLCLDQVLFIPTGDPPHK
                     QPDTLASAQHRYRMVQLAIQDEPAFTLTDIEICRSGKSYSIDTVRALQEQYGSDTEIF
                     FIIGLDAFLDLPSWKDAAQLLSLCHFVVISRPATTFLTVA"
     gene            complement(26533..27654)
                     /gene="proB"
                     /locus_tag="PK589_07080"
     CDS             complement(26533..27654)
                     /gene="proB"
                     /locus_tag="PK589_07080"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNJ19401.1"
                     /translation="MRDELLKQARRVVIKIGSSLVASRESGLSTERIERLASEIAHVR
                     AQGREVLVVSSGAVVSGIKKLELREYPKSLPVKQAAAAVGQSLLMWAYEKAFERLSLR
                     VAQVLLTHEDLADRRRFLNARHTLTTLIKFGVIPIINENDTVAVEEIRVGDNDTLAAQ
                     VAHLVDADLLIILSDVDGLFTADPRKDLQATLIPLIQEITADIEQRAGMSSTFEGTGG
                     MATKVRAAKKVGEYGVATLILNGTRTGLLPDVLSGQPGGSLFLGRERRMNSRKHWIAF
                     TLRPRGHVQLDQGAVDALVRNGKSLLASGIRDITGQFEAGDPVTCAGPDGKECAKGLV
                     NFSSTILTRIKGLKTADIQKIPGLQEYEEVIHRDNLVLL"
     gene            complement(27636..28667)
                     /gene="obgE"
                     /locus_tag="PK589_07085"
     CDS             complement(27636..28667)
                     /gene="obgE"
                     /locus_tag="PK589_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNJ19402.1"
                     /translation="MSTFVDQVQILVKAGHGGNGACSFRREKFVPRGGPDGGDGGDGG
                     SVIVEATTRLSTLLDLRYQKHYEAENGEVGGGSNCQGRRGADVRIPVPVGTIVLDANT
                     QELLADLTADGESCVIAKGGLGGRGNSRFATSTNRVPTQFEPGTPGEERLLRLDLKLL
                     ADVGLVGYPNAGKSTLIAAVSAARPKIADYPFTTLTPNLGVVRSTGEHSFVIADIPGL
                     IEGAHEGKGLGFQFLRHIERTSLLIHVIDISEWATEEPVASLKVMRHELSAYDEALAA
                     RPFAVVGTKLDIKGEGERLEQLRKYCQRRKIPFFAISAATREGLDECILYMGQQVELL
                     RKTPCETNS"
     gene            complement(28795..29058)
                     /gene="rpmA"
                     /locus_tag="PK589_07090"
     CDS             complement(28795..29058)
                     /gene="rpmA"
                     /locus_tag="PK589_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNJ19403.1"
                     /translation="MATNKGGGSTRNGRDSNPQYLGVKAYGGETIKAGSIIVRQRGTK
                     FFPGLNVGLGRDHTLYAYVSGVVKFEGGRGRQKVSVYPVTAKA"
     gene            complement(29080..29394)
                     /gene="rplU"
                     /locus_tag="PK589_07095"
     CDS             complement(29080..29394)
                     /gene="rplU"
                     /locus_tag="PK589_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNJ19404.1"
                     /translation="MYAIIETGGKQYRVETGTVLQVESLPGDVGHSVQIDKVRLLNGD
                     SGLVIGQPVVAGARVTAEIIRQGRTRSITVFKKQRRKNYRRTRGHRQGFTQLRVTEIA
                     SK"
     gene            29605..30519
                     /locus_tag="PK589_07100"
     CDS             29605..30519
                     /locus_tag="PK589_07100"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNJ19405.1"
                     /translation="MLQTSEKIWMDGKFVAWADAQVHVLTHSLHYGLAAFEGIRCYKG
                     TSGSAIFRLPEHVDRLFESAHIGLIEIPYDRKQMTDAIIETVRVNRLEACYIRPLVYI
                     GYGAMGLYPGENPIKVSIAAWKWGTYLGDEALQKGIRARVSSFTRHHVNVSMTRGKIS
                     GYYVNSILAKREVKADGYDEAIMLDPEGYVAEGTGENVFIVRRGVLKTTPLTSILEGI
                     TRNSIIQLAKERNIPVVEERFTRDEMYVAEEVFVTGTAAELTPVTEIDQRRIGTGKPG
                     TITQTLQKAFFDVVRGTDAAHSQWLTPV"
     gene            complement(30760..31113)
                     /gene="secG"
                     /locus_tag="PK589_07105"
     CDS             complement(30760..31113)
                     /gene="secG"
                     /locus_tag="PK589_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNJ19406.1"
                     /translation="MYTLLIIIHVIVCLLMIGAILLQSGKGAEIGAAFGGSSQTVFGS
                     RGPANFLSKFTVITAFVFMFTSLSLAILAKDRNFSSTVIDLNKKPAATTPSTTPSETA
                     TPPAKDSHSSTDSSH"
     gene            complement(31124..31903)
                     /gene="tpiA"
                     /locus_tag="PK589_07110"
     CDS             complement(31124..31903)
                     /gene="tpiA"
                     /locus_tag="PK589_07110"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNJ19407.1"
                     /translation="MRTRFLIGNWKMNKTATEAGAFVRRLTQELPRSEGIQVGFAPPF
                     TALHAAHEALGSSPDFLLGAQDLYWEDKGAFTGEVSGAMLKDLGCQFVLVGHSERRQY
                     FGEQDSWTNKKVLAALRNGIQPVLCVGETLHDRDTNQTDQVIQRQVRAGLAGIEGQHL
                     ESMVIAYEPVWAIGTGRAASPEQAVPVHRLIRQTINELSGAEAGARVRILYGGSVTPH
                     NIAEFLASEEIDGALVGGACLDPVSFATLAKVAIGSKPTTA"
     gene            complement(31939..33138)
                     /locus_tag="PK589_07115"
     CDS             complement(31939..33138)
                     /locus_tag="PK589_07115"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNJ19408.1"
                     /translation="MNIRKRIIEDLQLRGKRVIIRADYNVPLDDSLQITDDTRIRSTL
                     PTINRAVDEGAKVILCSHLGRPKGKFDPKFSLAPVAKRLQRLLGKEVIFAPDCIGSAV
                     EGLVAKMQPGDVMLLENLRFHPEEEKNDEGFSKALAALADVYINDAFGAAHRAHASTV
                     GITKYIPEAAAGYLLKKEIEYLEGAVENPVRPFVAILGGAKVSGKIGVIENLGKKVDK
                     VIIGGGMAFTFLKAMGLEIGQSLVENDMLDFAKGVQDHAFASGVKFYLPVDCVVAAGR
                     EPGAETKIVPVQEIPKGWYGLDIGPASVKLFSEAVQDAKTILWNGPMGMFEVDAFARG
                     TLAMAHSVANAYALTIVGGGETALAIHRAGESESISFISTGGGAALELLEGKTLPGLA
                     ALPNRAA"
     gene            complement(33235..34242)
                     /gene="gap"
                     /locus_tag="PK589_07120"
     CDS             complement(33235..34242)
                     /gene="gap"
                     /locus_tag="PK589_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNJ19409.1"
                     /translation="MTTRIGINGFGRIGRNVLRASLGDPSLEFVAINDLTDAKTLAYL
                     LKYDSVHGTLDASVEAKDDQLIIDGRAIKVLAVRDPKELPWKALGVDIVVESTGHFTD
                     REGAGKHLSAGAKTVIISAPAKDPDATVVLGVNEQVFDAKAHHIVSNASCTTNCLAPV
                     AKVLLENFGIKHGVMTTIHSYTNDQQLLDLPHKDLRRARAAGMSMIPTSTGAAKALHL
                     VIPQLKGKLDGLAIRVPTPNVSLVDLTVETEKDCDVAGVNAAFKKAAEGPMKNVLAYS
                     EAPIVSIDLKDDAHSAIVDAPLTAVIDKRLVKVTAWYDNEWGYSCRVRDLIKYIVAKS
                     A"
     gene            34458..36890
                     /locus_tag="PK589_07125"
     CDS             34458..36890
                     /locus_tag="PK589_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNJ19410.1"
                     /translation="MQSEKLQFEACKVLEWAKLLDLLAGYAQSEVGAEQCRSMELERD
                     VAQARLRQEETSDMLRLRASVDPFPVLRFPDVTEWLKRVVKGACLEAHELRDIALVLE
                     LVVDVQRYLLRHQSDAPTVGAMAEQLGPVMEYRRLTVALNGAIDPDGSIRESASPELH
                     RLIQRANDFKQQMRHRLEQILHSRRYEEVLQERYFAQREGRYVVPIKAEMQGRMPGIV
                     HDVSSSGATLFLEPRELVDLNNSIKVVDLDIEREVRRILRELSALVAPHQPALAGSVT
                     LLGRLDAISAKAGLSQRLQGSPPRLNDQGRILLTQARHPFLVVSKDQVVSNDLAFDET
                     VRVLIISGPNTGGKTVILKLLGLFSLMVRCGLHLPCAPESEMAIFPSVYADIGDAQDL
                     ARDLSSFSAHITQMVQLLNEVSDGAGSEHDREPVPPGRALVLLDEPVTSTDPAEGAAL
                     ASALLCRLAAAGVKVVATTHYSLLKGLAQDRPGFANASVEFNLDTLSPTYRLIQGQPG
                     GSAAIEIAGRLGMDQSLLEDARARLQGDNRLLETLLSDLQDKQRRLNDDLVAATAARQ
                     GAEQASREAQELLTFLQESERDERQGLKKKLSQEFQRARAAVQATMDDLKRDQKLLKA
                     KEAKARLVELEESVRREAGDSEEPIPIDQLTIGDAVEVIGLGMTGTLLENPQGKKRVR
                     LKVGEGEILANVASLAGVSKARQPQAPAAVKTSVVSPRRTSQTLAPEDIQETLDVRGQ
                     AADDALDVVVAGLDRAILGGSPFVRIIHGHGTGRLRSALRDYLKDSPYVVAFRGGDRA
                     EGGDGVTIVQLR"
     gene            36999..39140
                     /locus_tag="PK589_07130"
     CDS             36999..39140
                     /locus_tag="PK589_07130"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNJ19411.1"
                     /translation="MPFSPRTKKILSAIAVGVAVYALVTGLRMTRWFEVVELKAFDHL
                     VRRYADPAKADSSLVLLAIDESSLEAFGRWPWPRDRYGYVVRYLKRAGAKAVVFDVMF
                     FEPDDNAEEFDQSFADDVKAAGNVFLPMLFQSEAAPVPAGLQDHATVKAERSDSGPVQ
                     AAAHPGVKLPIPVLAQQARGLGVINLSADADGPTRRLPLLGQVQGNLVPHLSLAVARY
                     VLGGSELAMRDDRLQIGDSSVPLDADGNLLIKWHGSLEQTYHARRYSIGRVLQAFAQQ
                     EKGERPDLDASLFKDKIVFIAGTAAGLYDLRVTPFSSATPGVLIHMAALDNLLHGQGL
                     QAAPSWFSLSILLLLCLVSAGAFMLFPSYPVKFGVTIGLALAYYGLVAHAFAGHERWL
                     DLVFPEVALALTFGTAATVEYVTEGKQRRLMRAAFDKYMSSEVVEEIMRNPAAIKLGG
                     EKKEISILFSDIAGFTTISEKMSPEDLVALLNRYLSAMTTIIKTTHRGNVNKYLGDGI
                     MALFGAPLNDPQHASLACYAALDCQVELARLRQVWKQEGLPEIGARIGINSGACIVGN
                     MGSEERMEYTVTGDSVNLASRLEGASKYYDTLILIGQRTAELAKNDVEVREIDLLRVK
                     GKKDPVVVFELLARKGQVEEKKRRVIDVYLEGLAAYKVRNFSIACAKFSEAVTLDPSD
                     GPSRVYLDRATNYCQTPPSADWDGVYEMTSK"
     gene            39167..39679
                     /locus_tag="PK589_07135"
     CDS             39167..39679
                     /locus_tag="PK589_07135"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNJ19412.1"
                     /translation="MISPRVLRVVLIVLWIGLLVWSGEGRAETVYVQAKTAQLRAGKT
                     SLDAVVGNVKFGDALEVVGRDGSWVEVKTSAGAKGWIFANKTSSSKPSGTNDTLAKLG
                     QSMRGSDASATTASAGARGLDKASEGYANRAGVSARDRDAVDRMTAYQIPDQEVEEFL
                     REGGLGEYAK"
     gene            39669..40535
                     /locus_tag="PK589_07140"
     CDS             39669..40535
                     /locus_tag="PK589_07140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNJ19413.1"
                     /translation="MRSNHGLTATIIALSLMVAGCAEVQRASEDIARQSGNPRLAGAI
                     HGVGNVVGSLFPIGYEEESSIGQAMALQVVARYGGVVDQPELVRYVNLVGRAVANTSD
                     RPDIPYRVAILEHESINAFAAPAGYIFVTRGLLKQVRNEAELAAVLGHEIAHVSQKHI
                     LDVIQRSKRLAGVTEAGLSYATSNPAAFKTVIDGAVKKLLDEGLDQDKETEADTIGEV
                     FATRVGYDAEAYVNLLTRLRDLKGDDRALFKTHPNFSARIEAVQKTIQSKHLVATGVL
                     LPERFARMTKRV"
     assembly_gap    40618..40717
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(40996..42909)
                     /locus_tag="PK589_07145"
     CDS             complement(40996..42909)
                     /locus_tag="PK589_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ19414.1"
                     /translation="MRAARRTGGLQRKFFVALLIVGIVPGMAALWATYRSSTASLKEA
                     IGEGFQEIARSTSIRLAGAVDDEIDRAGRLAISVANQHAQAQSKADSNKTEAARVHST
                     HRPVNSPVTAAYLEDWAREARHYLRVTVADHRGLVIASTDRALPPQQADQSWWREAMQ
                     APAGTAYVSNVTIDPAINDATFHVAVPIVEKAGGAVTGAVDLVIRHTLLTQLILPIHI
                     GNTGHAMLLDTQGTPLICPVLPPTAHLIPANLMNRLALDHPLWLVAEDDAHGGRDAIV
                     GASPVRFSHPLTRSSLEGNRWYAFVRQAPEETYAPIYSLLLSVGVIGFGLVIALSMFG
                     FVVGYRIVRPLTALQREAEGLRLTLATTQTDHALPLAATPLPREYRTGDEIEDLATTF
                     SAMRAVLEENLRTIRAQQHELIRQEKLASVGQLLAALAHDLRNPLGVIRSSAQVVLEG
                     PQEEGIRHEMARYIIDEVDKLSQRLNDFLRYARQKPPDLADCAAEGIIGAALKQWEAQ
                     GGHEHIRVEQRFSHDLPMIRIDPEQIKDALVNVLINAREAMTEGGGTLTLTTRSAEGG
                     TVEINVTDTGSGIAPDHLGRIFEPFFTTKTYGTGLGLTNVKRLVEDNGGTLIVTTNQG
                     KGTTCTLRFRSAT"
     gene            complement(42909..44294)
                     /locus_tag="PK589_07150"
     CDS             complement(42909..44294)
                     /locus_tag="PK589_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ19415.1"
                     /translation="MHAPTLLIVDDEERMRRLFELVLKAAGYQLLLARSGDEALHMLQ
                     EHDSIDMIITDLQLGTVSGMDVLEAAHKQAPDVPVLIVTGYGTVKSAVEAMQKGAYDY
                     ISKPVDNEELKIVIARALQVRQLARDNRTLRAGLQEQFGFDRIVSVSKEMELVKRLAK
                     EVAQTDATVLITGESGTGKDLLARAIHLASPRAQGPMVALNCAGIPEHLLESELFGYE
                     KGAFTDAKKSKPGRFQMADRGTLFLDEIGELSLTAQAKLLRVLEQHVVEPLGGVRSIP
                     VDLRVIAATNQELPDLIKAGRFRLDLYYRLNVYQLRMPPLRERPGDIEPILTQFLDRA
                     RRERGSRIKGIAQNALALLTQYPWPGNVRELHNVVEWLTITCKQDMIQPEHLPASLTA
                     AAPQSSASPAGAPSLLSLGLSVEEVEKAMLQEALAKSGGNVSEASRLLKITRNTLRYR
                     MAKHNLSQPQG"
     gene            complement(44384..45424)
                     /gene="queG"
                     /locus_tag="PK589_07155"
     CDS             complement(44384..45424)
                     /gene="queG"
                     /locus_tag="PK589_07155"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNJ19416.1"
                     /translation="MRETVMPEPLTALITQAARELGFDVVGISRLPPQPAPNHNPSVS
                     PHRLLDRLHEWLQRGFHASMNWMTRDPERRADPALVLPGCRSIISVGMNYYTDHRADE
                     RPGNGRIARYAWGLDYHNIMGDRLTQLSERLATLAPEDRHRAYVDTGPVMEKAWAQQA
                     GLGWIGKHSNLVSPEFGSWLLLGEILTTLELDADEAGTDLCGSCTLCIQACPTGAITE
                     PYVVDAGRCISYLTIELRGSEPTLSEELRRGIGNRIFGCDDCLDICPYNHQAEPTSEP
                     GFQPTPLTTSPSLRSLMDMNEATFAATFKQSPVRRAKHAGLQRNVSWALANEAAPAGN
                     GTPASSSSRDSR"
     gene            complement(45409..45783)
                     /locus_tag="PK589_07160"
     CDS             complement(45409..45783)
                     /locus_tag="PK589_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19417.1"
                     /translation="MSWLVRLISPVLLSVQIALHVMPFDAQAQAPADSAATTEHSCSF
                     GMAKGDPAHQCQVPIPAGCVIATFPGTDKPWTTVSKAGRTFCAFDKKGTDWKNKITGQ
                     CTRCDSSHCTGQFMVRFECAKP"
     gene            46053..46859
                     /locus_tag="PK589_07165"
     CDS             46053..46859
                     /locus_tag="PK589_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ19418.1"
                     /translation="MTGNNPVFKPFSDCTMAQEKILIVDDEVVVAEDIRRQLRSLGYV
                     VVGVVASGSEAVDMAGEHRPDLILMDIKLKGPLDGIDAARMIQARYGTPVIYLTAFSD
                     EDTLKRARYTLPLAYLIKPFVSSDLRAAIELALFRHRVLRIAEQRGRWLDSVLQSMSE
                     AVVTIDAQGKVTLLNQAAEGLTGWSQPDALGKPIQDVMVVVDAERRTPLVNPALSVLQ
                     QIKAGDRGTRPFLLLSRQGVEHVIHESATVIHDEQGGLTGAVLVFRLAIE"
     gene            47135..47728
                     /locus_tag="PK589_07170"
     CDS             47135..47728
                     /locus_tag="PK589_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNJ19419.1"
                     /translation="MVQLMSFLGTLLGCLMAFVLATTPQSWAGEVGSIHLLRPLLVQS
                     EPGGGVKATATLLFPGSPSVLHSILTEYRKWPELFETRMRMAQVEEREGHVLTDVYIS
                     HALLPGEQRLLCESQVLPGGGLVTDLKGGDFKQYHREWRLQAAGEGTQTRAEFELLVE
                     PKTMIPDWLIAVAMERELTVHFRLLRQRALDLVTKGR"
     gene            complement(47712..48890)
                     /gene="thiI"
                     /locus_tag="PK589_07175"
     CDS             complement(47712..48890)
                     /gene="thiI"
                     /locus_tag="PK589_07175"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HNJ19420.1"
                     /translation="MHCAIIHYHELALKGRNRDFFEQRLVRNIRLALKDLKIRRIESL
                     QGRIRVTIPEDVTPDLVTERLRRICGVSNFLLSESVPLDIREPELSSIKQVAQRQLGT
                     QTFTSFRVTAKRADKRLPLTSMDVEREVGAYLHEAMGKAVKLKQPDVTLYIELLTKEA
                     HVAARKIEGPGGMPVGTSGKVACLISGGIDSPVAAYRMIKRGCKAVFVHFSGRPLVSR
                     ASEEKVQELVQLLTTYQYHSKLYIVPFGEIQQEIVANAPAAFRVVLYRRIMVQIAQEL
                     ARREGCWALVTGDSLGQVASQTAENLVVVENAAEIPLLRPLIGMDKIEITEQAQQIGS
                     FTTSIEPDQDCCKLFVPLHPSTRTKLEDIVRIERGLEISAFVKRGVEKAELTTFTFPS
                     "
     gene            complement(49194..50120)
                     /gene="trxB"
                     /locus_tag="PK589_07180"
     CDS             complement(49194..50120)
                     /gene="trxB"
                     /locus_tag="PK589_07180"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNJ19421.1"
                     /translation="MHHLVIIGSGPAGLTAAIYAARANLSPLLIEGWQSGGQLTTTTE
                     VENYPGFSKGIMGPELMKEMRGQAERFGTEFLTGDVSAIDLSTRPFRLTIDGEQTIGA
                     SALILATGASPIRLGLPNEHRLTGYGVSTCATCDGYFFRGQDLVVVGGGDSAVEEATF
                     LTKFARTVTLVHRRDKLRASKIMQDRAMNNEKISFAWNSIVEDILGKDLVTGVRLKNL
                     VTGHSTDLPCAGVFVAIGHRPNTDLVKGQLEMDEQGYILTSRGTNTSVPGVFAAGDVQ
                     DAKYRQAITAAGSGCMAAIDAERFLESALHSL"
     gene            complement(50230..50850)
                     /gene="coaE"
                     /locus_tag="PK589_07185"
     CDS             complement(50230..50850)
                     /gene="coaE"
                     /locus_tag="PK589_07185"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247713.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNJ19422.1"
                     /translation="MVSSRPMIFVGLTGGVATGKSTVAKMLERCGALVIDADALAHQV
                     MEPGKPAWRAIVKTFGPAVINPDRTINRPALGTMVFRNRARLRRLEQIIHPRVAREQA
                     RLTRLAVRKDPNAVVIYDVPLLYEAGIDARVDKVIVVTADRETQIARLKKRNGLTRSE
                     ALRRIRNQWPLRKKVAAADFVLDGTTARPRLFTLVKSLYRELARLA"
     gene            50916..51947
                     /locus_tag="PK589_07190"
     CDS             50916..51947
                     /locus_tag="PK589_07190"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HNJ19423.1"
                     /translation="MKIMNYWILQIAFLCVSVGTVSMVSVKAEEPPAERQPLVVALDG
                     SGQYRSIQEAVDAAKKGDTILIRPGAYPEDVTIHSKENVRLIGAGMDQVTILGRERVG
                     VFHIGKWPYGATNIEISGITVNEHGGHAMGMFNGRGIHFHHARVKGMLFTQQVEDVRI
                     ENCDIGGSETTGVQFANSQAVMRGNFIHDNDHGVSVAGKSTVRLEHNVITRSLFEAVI
                     VNDQAQAVLVGNTFVKNGGGAAFLGTSQNEASGNIVSLNTYGFVVAASSRVLFSYNAM
                     QNSGSNYLRSGTPNQPAPELKPDSDLTVDPRFVDTAQDDFRLRADTSLVKIGTFPYLG
                     ALPPLSASR"
     gene            52104..52289
                     /locus_tag="PK589_07195"
     CDS             52104..52289
                     /locus_tag="PK589_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19424.1"
                     /translation="MASFRFDLEKRSTDGIPNLAEVESGKLLGAILPMSEQAQLQLRD
                     SIEVIDYLMNQETVVWS"
     assembly_gap    52525..52624
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            52689..>54211
                     /locus_tag="PK589_07200"
     CDS             52689..>54211
                     /locus_tag="PK589_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HNJ19425.1"
                     /translation="MSTSTGKKSVPVPPSAEQQRLDDDDLRRKHWKRWGPYLSERAWG
                     TVREDYSPYGTAWEFLPHDHARSKAYRWNEEGLAGISDRHQFICLALALWNGRDPILK
                     ERLFGLTGNEGNHGEDCKEYYFYLDSTPTHSYMKFLYKYPQAAFPYTRLIEENRRRNR
                     TDPEFELLDTGLFDDDRYFDIVVEYAKASPEDLLLRITAINRGPEPSELTVLPTIWFR
                     NTWSWGIDVRRPRMRQGESSPGVSAVEFDHEYYGRRRFLCDGAPDLLFTENETNTRRL
                     YGDSDGARYVKDGINDYIVQGDKNAINPDRIGSKAAAHYVRSAMPGQPVTIRLRFTNG
                     SAEGALDPEAFDAVFAARLKEADEFYAHLAPPDLSADARLVQRQAFAGLLWSKQFYHY
                     EVDRWLKGDPAGPEPPPERLRGRNADWTHLYNADVISMPDKWEYPWYAAWDLAFHCLP
                     IALVDSRFAKDQLVLMLREWYMHPNGQIPAYEWALGDVNPPVHAWAAWRVYKIEKKR"
     assembly_gap    54212..54311
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <54312..55190
                     /locus_tag="PK589_07205"
     CDS             <54312..55190
                     /locus_tag="PK589_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HNJ19426.1"
                     /translation="ASKFFEHFVYICRAMNNIGNAKIELWDREDGFFYDVLHLPDGKT
                     FPMKVRSMVGLIPLFAVETLDSELIDQLPRFKHRMQWFIENRPDFGLHIETQSQDGEV
                     RRFLSLVNRRRLAKVLRYLLDEQEFLSPHGVRALSRYHKDHPYVFSMMGTSHCVEYQP
                     AESSNGLFGGNSNWRGPVWFPVNYLLIESLQKFHYYLGESFRVEYPTGSGQRRNLAEV
                     AAELSRRLTHTFLRGPDGRRPVYGGTAKFQQDPHWRDLLLFYEYFHGDNGAGLGASHQ
                     TGWTGLVAKLIQQSGE"
     gene            55205..>55602
                     /locus_tag="PK589_07210"
     CDS             55205..>55602
                     /locus_tag="PK589_07210"
                     /inference="COORDINATES: protein motif:HMM:NF023855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HNJ19427.1"
                     /translation="MTIDSRHCQDLDGALNLEWLETNGRGGYASGTVAGANTRRYHAL
                     LLIARHPPVDRVVLVNHVEEFVESGGRTFPLSTNLYHDAVHPEGFQYCEEFSADPWPT
                     WRYAGHGIRLIRELLCPQGRDLVILRWRLL"
BASE COUNT        10740 a        15342 c        17244 g        11876 t
ORIGIN      
        1 gcccgcacat gctgcagaaa tgggcgacct tcgccgcatt gtccggcagc gtggcatcgt
       61 ggtacgagcg ggccgtctcc ggatcgagcg caaggttgaa ctggtcttcc caacggaatt
      121 caaaccgggc cttcgacagg gcgttgtccc gggcttgcgc gccgggatgg cctttggcga
      181 ggtccgccgc atgcgcggca atcttatagg tgacgacacc gacctttacg tcttctttgg
      241 tcggcaatcc caaatgttcc ttcggcgtca catagcagag catggcgcag ccgtaccacc
      301 cgatcatcgc ggcgccgata ccggaggtga tgtggtcgta ccccggcgcg atatcggtgg
      361 ttagcggccc gagtgtatag aagggcgctt cctggcattc tttcagctgc ttctccatgt
      421 tctcttgaat catatgcatc ggcacatggc ccgggccttc gatcatcacc tgcacatcgt
      481 ggttccaggc gatcttggtc agttcgccaa gggtctccaa ttcggcgaac tgtgcttcgt
      541 cattggcatc cgcgatggag ccgggacgca acccgtcgcc gaggctgaac gccacgtcgt
      601 aggctttcat gatctcgcag atttcttcga agtgcgtata ggcgaagttc tcctggtggt
      661 gcgagaggca ccatttcgca tggatggagc cgccgcgaga gacgatgccg gtcatgcgtt
      721 tcgccgtcat cggcacgtag cgcagcagga ctccggcgtg aatggtgaaa tagtccactc
      781 cttgctcggc ctgctcgatg agggtatcgc ggaaaatttc ccaggtcagg tcttcggcct
      841 tcccgttgac cttctcgagc gcctggtaaa tcggcacggt cccgatcggc acgggcgaat
      901 tgcggatgat ccattcccgc gtctcgtgaa tgtttttgcc ggtggagagg tccatcaccg
      961 tgtcggcgcc ccagcggatc gaccagatca tcttctcgac ttcttcttcg atcgaagagg
     1021 cgacggcgga gttgccgatg ttgctgttga tcttcacaag gaagttccgg ccgatgatca
     1081 tgggctcgga ttccgggtga ttgatattgt tgggaatgat agcgcggcca cgggcgactt
     1141 catcccgcac gaactcgggg gtgatgaggc tcgggattcg agcgccccag gcttggcctg
     1201 gatgttgtga gacacccccg ccatgcccgt tgctcgacgc catcaagtcc ttcgcgcggg
     1261 cccgggcttg gttctcccgg atcgcaatga actccatctc cggcgtgatg atgcctttct
     1321 tcgcgtagtg catctggctg acgttccgtc cggccttcgc gcggagcggt ttgcggatgt
     1381 gcgtgaagcg caggtccgcg agcttcggat cagccgcccg ttgacggccg tattgcgacg
     1441 tcacttgcgg gagttcttcc gtatcctgcc gggcttcgat ccacattcgg cgcaagggcg
     1501 gtaacccggc gcggacatcg atcgtgacat tcggatcggt ataggggccg gaggtgtcgt
     1561 acacggtcac cggttggttg ggcgtgccgc cattgccgtt ggtggagtgc gtcggtgtaa
     1621 ggctgatttc acgcatcggc acgcgaacgc cgggttggga cccttcgaca tgcaccttgc
     1681 gcgaggccgc gaatggggtg gtggtcaggc gcgccgtcga gggtttggcg ccgtgtccgt
     1741 tgctggaacc gttctgtgta tgggcctcgc tcatggcgga attcctttcg tcgaaggtga
     1801 gcggtctgat ggccaaaata aaaaagccgc atccagcttg ggtgcggctt gaagcgatct
     1861 cgaagccagt gaaccggtcg cttccctacg ctggtattac ccaggtcagg ttgtgagggt
     1921 cgcccgatcg ctcgggctct cagccggatg gcacccctag ctgtcgttgg tgatcgtacc
     1981 gacctgtcag ggcgaagtca atcttctgga aggcctagtg gcaaggtgcc tcatggctca
     2041 tgtggacggt cgttactcgg caataccagc gcaattggct ccgagccagc ggctcttgaa
     2101 gtcgatgtga actttgccgg cacggggacg gtcggtgtcc atgcttccca cgagcaactc
     2161 tgccgtctgg gcttccacgc tcatccggga cgttgaggcc tgaggagcag gacacgttcg
     2221 ttcaaggacg agtttcgcgg atgatttcga caccaccgag gttttacagc ctggctcatc
     2281 atcatcattt tctttgatga tgcgattctg gtccaggtcc tctttggtca ggcaggtttt
     2341 gacggaatgg gtcttcggtt tgccggaacg tgcctgcatg gattgttcga ctttggcacg
     2401 ttgttcaggc ggcatttttg ccagaacatc cggtgggacg agcatgccgc tggattgagt
     2461 ggtgaaagtc atctcccagg cgccgggttt cgcgttgaat gattcggcct gtgccccgga
     2521 ggtcggccag agcaggacgg ggttcgacag aagtccacat gccaccaggt acacaaagcg
     2581 gccaagcgtc ggtgtcatcc gttttttcat gggtcggtct cctcctctcc ggggtgacta
     2641 ggatggtgcc tgcgcaatca tacgcagttc tgtcggagaa atcacgatgt gtgacagcac
     2701 actcgctttc tctcctgagc gggcgtaagc ttttcttaag accaatgatg cactgcgctc
     2761 acagaaatgg aggcacatca tgaacgggag aaatttttgg atcgggctgg tcgtgggagc
     2821 gagtgcgatg ggaattcttg gtcagtgggg gcctgccgtc gcgggcaagg acgccattca
     2881 acccacctgt atccccgccg ccgcggtggc cgaatatctg cacagcgtca ttcaggccga
     2941 tcgtaccttc tacaccacgg atgtcgtcga gcggatgcag atgcgcggga ttgcctttgc
     3001 cgcggaaaac tggcgtgaaa cctcgcggct tcctcttccg gcccaatatt tgttggaaac
     3061 ggggcggctc gtggcggcgg gacgaagcgg ggtgcaatac cggttgatca gcaactgggc
     3121 gatcaatgcc aagaaccggc cgatgaccga ctttgaacga gcgggcctga cggagatcct
     3181 cgtcaatcct gatcagccct atacggcagt gaccacggag ggcggcgccc ctgtttttca
     3241 agccatttat gccgacaaag cggcctccca gtcctgtgtc ggttgccaca atgcccatcc
     3301 caatagtccg aagaaagatt tcaagccgca ggatgtcatg ggagggattc tgctcaccat
     3361 tccgctgccg cagtagaggg atgtgcctgt tgaggaccta gaagggttgc cacgagactt
     3421 gttgaagtcc gacatgtgga tgagtccatg gatcacctct cacattcctc tcgcatcgcg
     3481 cgcccccttc gcccaattgc cccagttcct tctaggccat ctgctccgac tcatttcatt
     3541 gcgtagccgc gaaagggtgt cgtagaatta ttaagaccgt gtctcttcat gacacagggt
     3601 atggactcaa ccgaaaggct gcaggtgaaa acggtcacca cgcttccccg tcccattacc
     3661 gactatcagt ctctgtctgc cgaagaactc ttcgaacgga cccgcgcggc caaacgggcg
     3721 ctcggtgacc gggtcatggt tctcggccat aattatcagc gcgatgaagt catccaacat
     3781 gccgacttcc gtggcgactc gctgattctc gcgcaagtcg ccgagcagcg ttccgatcgg
     3841 ccctatgtgg tgttctgcgg cgtgcacttc atggcggaaa cagccgatgt gctgagccgt
     3901 tccaaccaga ctgttattct ccctgacatg gcggccggct gttctatggc cgatatggcg
     3961 gcgatcgagc aggtcgaaca gtgctgggat gcgctcggtc gggtggtgcc ggtggaggag
     4021 acggtcatgc cggcggtcta cgtgaactcc gcggcgatct taaaggcttt ctgcggcgag
     4081 catggcgggc tgacctgcac ttcgtcaaac gcgcgcaagg tgatcgaatg gagctgggcg
     4141 cgacgtgaga aaatcctgtt cttccccgac gagcatctgg gccggaatac ggcgaacaaa
     4201 atgggcatcc cgcgcgagca gatgatcgtc tgggatccgt atatgccgcg tggcggcaac
     4261 tcggtggagg cgattcagcg cgcgaagctg atcttatgga aaggccattg cagcgtgcat
     4321 cagatgtttc agccgtccca catcgattac gtccgcaaga agcatcccga ggtaaaagtc
     4381 attgtgcatc ccgaatgtca cgaagatgtc gtcaacaagg ctgatctcgt gggatcgact
     4441 gagtacatca ttcgcacgat cacggcggcc ccggcgggga ccacctgggc ggtcgggaca
     4501 gaactcaacc tcgtcaatcg cctgaagcat gagcaagccg ataagaagat ctttttcctc
     4561 tcttccacgg tgtgccaatg cgccacgatg tatcgcatcg acgctccaca tctctgttgg
     4621 gcgctggaga atcttgccga tggacacgtc gtcaaccgga tcgtcgttcc ggacgacgaa
     4681 aagcaactgg cgaaggtggc attggaccgc atgatggcgc tcagctagca aactgttgag
     4741 aaaggccgct ggctgcgttc tcgcgtcgct cgcatgttca acgtaccgca agggtacgcc
     4801 tcacatgctc cctcgctgcg gccttgccgg tcgacctttc tgaacagctt gcaggacagc
     4861 caggtcctac cagtcacctt gctaatggtt ccggctgcca acaaaccact ggttcctctc
     4921 gccccgattc ggtatagtcc tctctttacg cgtacttcat ggccatttcg tctcgcagct
     4981 agatcaatct atggactata aatcgaccct caatttaccg aagaccgact ttcctatgaa
     5041 ggcgaacctg ccgcaacggg agccggagat gctggcccgt tgggcacagg aacggctgta
     5101 cgaacggatc caggagtctc gccaggggcg tgaacaatac atcctgcatg acgggccgcc
     5161 ctatgcgaac gggcggatcc atattggcca cgccctgaac aagattctga aagacatcat
     5221 cgtcaaatcg aagacgatgt ccggttatca ggtgccgtac gtgccgggct gggattgcca
     5281 cggtctaccc atcgagcacc aggtcctgaa ggaactgggc gacaagaagc gcgacctgga
     5341 tgcgctcgcg atccgcaaac tgtgtcagga gtatgcggcc aagtatgtgg ccatccagcg
     5401 ggaggaattc cagcggctgg gcgtgctcgg cgaatggcag caaccctatc tcacgttgaa
     5461 tccggcctac gaaggcacca tcatccgcga gttcggccgg tttgttgagc gcggcggcgt
     5521 ctataaaggc ttgaagccgg tcttgtggtg cacgcaagac cagaccgcac tggccgaagc
     5581 cgaggttgaa tacgacgacc acacctcgcc ctcggtctat gtaaagtttc ccctggtgag
     5641 cccgccttct gtgttgagca agaggtttgg gccggtttcg ttccctgtcg aagcaacacg
     5701 cgcgtcggtg ctgatctgga cgacgacgcc gtggaccctg ccggccaatc aggccgtttg
     5761 tcttcatgcc gacatcgact atgccttcgt gctggtcggg gacgaattgc tggtcatggc
     5821 tgagaagctt gtcgagtcgg ttgccaaggc gtgcaacatc cccacgtaca cgatcgtcgc
     5881 cgtgaaaaag ggtcgcgagg gatttgaggg cttggaggcg caacgcccgt tgaccacagg
     5941 tctctctcca ttgctcctgg gcgatttcgt gacattggag cagggaaccg gatgcgtgca
     6001 catcgcgccg ggccacggca tggaagacta tctgctggtg ctggatcaca atgccaaggc
     6061 gagtgtcggc gagcgactcg aaatcctggc tcccgtggac aatggcggga agtttaccga
     6121 agccgttccg gagtttgccg gtcagcatgt cttcaaagcc aatccgaaaa tcgtggagcg
     6181 cctgaagggc aatggccgct tgctcggaca cggctcgctc aaccactcgt atccccattg
     6241 ctggcgatgt aagcagccgg tgatctttcg cgcgaccgaa caatggttcg tctctatgga
     6301 gacgaatgat ctgcggaaag aagcgttggc agagattgaa cgggtgcgct ggatcccggc
     6361 ctatggccgc gaccgcatta atggaatgat cgaaaatcgc cctgactggt gcctgtcacg
     6421 ccaacgggtt tggggcacgc cgatccccgg cttcacctgc ctcacgtgcg gcaaggtgct
     6481 ggcccatccc cgcgtgatcg atcatgtggc ggatctgatc gggcaacatg gcaccgacta
     6541 ctggtttgcc aaccaggctg gcgcgttgct gcctgccggc acgcagtgcg aaggttgtgg
     6601 cggaactgat ttcgagaagg agcgcgacat tctggacgtc tggttcgagt ccggcgtgag
     6661 ttatgcggcg gtgctcaagc ctcgacaatg gtatccggcg gatctgtatc tggaagggtc
     6721 ggaccagcat cgcggttggt tccacagcgc cctgttgtcg ggtgtgatca ccgatcatcg
     6781 tgcgccgtat aaagctgtgt tgacccatgg tttcgtgctc gacggggcgg ggcgaaagat
     6841 gtcgaagtcg gcgggaaacg tcgtggctcc tcaggacgtc atcaaacaat ccggggcgga
     6901 aatcctgcgc ctctgggtat cggcccagga ttatcgcgaa gatcttcgaa tttcacagga
     6961 gatcctgaat cacctgatcg aagcgtaccg aaagattcgc aatacctgcc ggttcctgct
     7021 gagcaatctg tacgacttcg atccggcgca gcatcgcgtg cccttcgagc agttgcccga
     7081 attagaccgc tgggccctca tgcggctgag cgacctgatt ccgcgcgtgc gcaaggggta
     7141 tgacgaattt gagttccaca cgatctttca cgcgttgaac aatttttgtt ctgtcgatct
     7201 gagcgccgtg taccttgata tcctgaagga ccgtttgtat acgttccgga aggattctcc
     7261 gctccggcgt ggttcgcaga cggtgttgtt cgacatcgtg gttgcgttga ccaaattgat
     7321 ggcgcccgtg ttgagtttca ccgccgaaga aatctggaga acgttgcctg agtctgtgcg
     7381 cacagaagca aaagccgaca gtgtgcatat cgccatgttc ccagaggtcg atccgcgctg
     7441 ggctgatccg atgctggcgc aacggtggga gcaggtgctc gaggtgaggt ccgccgttca
     7501 ggccgctctg gaagtgaagc gtcgcgagaa ggtgatcggc gcgccgctgg aagcgagagt
     7561 cgtgatcgag gcgaattcgg agcggtatga attcctgaag cggtacgaac aggagctctc
     7621 gtccttcttt atcgtgtcag atgtggttct cactcaggcc ggtcatctcg cggagtcccc
     7681 cgggtatagc gtgacggtgg ataaggctac gggcgccaag tgcgagcgct gctggaacta
     7741 tcgatccgct gtgggcacct ctgccgacca tcccacactg tgcgatcgct gtattgaggc
     7801 cgtccgttga gccagtccat ccgttatctg ctgctcgcct cgctgagcct ggcgattgtc
     7861 gtgttggatc aagtcacgaa ggtctatgtc atggagacca tgcggctcca tgagtccatt
     7921 ccagtcatcg ccaatctgtt cagtatcacc tacattcgca atcccggtgc ggccttcggt
     7981 ttcctctcat cgagtagcag ctcgttcagg tttgtcttct tcggcctgac atcgatcttt
     8041 gcggtgggct tgctcggcat gatcatggtg cgtatgccga aagaagactg gatggggcgg
     8101 ctcagcgtgg cgggaattct cggcggagcc atcggaaact tattggatcg gctccgttac
     8161 ggagaagtca tcgacttcct cgatttttac gtcaacgggt atcactggcc ggcgtttaac
     8221 gtggctgatt ccgcgattac cgtcggagtc gtgtttctaa tacttcactt cgcgacggag
     8281 aaggaaccgg cggatcagcc tgtcgcttcc gagcagccgc cttcctgacc agcgcatttc
     8341 cgaccatcac accggcaagg tgatgatgaa tccgccctgc tcccgaaact gccgttgctg
     8401 cttgaccgaa aacatcgcga gcggttcgct gtggcagaac tggcattcct tgagcgtaat
     8461 cacggcctga ggctcgccga ttcccaccag gtattgcttc gccagcgcca gggctttttc
     8521 ctgactggtc gtcataacat cgaagtgcag tttgccgttc tttcctttga cccacgtatc
     8581 gtacacgtgg acgctgtcca tgttacttgc catgacgatt cagctcccgg ttatggtttg
     8641 gttatcggac cacgagcagc acaatgacgc ccagtaccat gcggtaatag gcgaacacgc
     8701 tgaggctgtg gcgttgcaca aaggtgagaa aggcggcgat cacggcccag gcgaccacaa
     8761 acgacaccac caggccgatg ccgagcgcca cgtagtcatc ctgagaaaac gaggcctgcg
     8821 atttgagcat ctggtagatc gtggcgatga tcatggtcgg gagggccagg aagaacgaat
     8881 attcggtggc cacgcgccgg tcgaggccgg ccagcagacc gccgacaatc gtggaacccg
     8941 agcgggacat gccagggatc agggatgcgc attgggccaa gccgaccagc agggccgatc
     9001 gtggcgtcac ctgtagcagc tctttcacgc gcacgcggct ctgcattcgc tcgaccacca
     9061 gaatgatcac gcctccgacg attaaggaaa ttgccacggt cgtcggcgag aagagatagg
     9121 ccttgatcga cttgtgcgcg acgaatccca ccagcgcggc gggcaggaag gccaggccca
     9181 atccaatcac gaaccagaga ttgggctggg cattgatcga ttcctgaagg gtcgttgccc
     9241 aggatttcgt gccccgggtg gcgatcaacg agcgaaactc ctgttgttcg cgtagggcat
     9301 gggacgcgag cgacgccagc ttggcgcgct cgtaagcgat gatcgcgaga atcgagccga
     9361 gctgaatgga gatctccacg ttggaggcga tatcccccgt aaacccgagg gcatgcccga
     9421 cgaggatgag atggccggtc gaggatacgg ggagaaattc cgtcagtcct tcaatgatgc
     9481 cgagaatgac ggcaagctcc gggccccagt tcatcatagt gtgtgtgaga tggtgtgtgg
     9541 gatgctgcgg ttggttgcgt agttgtcacg aatcgtcgtt gcataatccc agactggtca
     9601 ccggcagtca agcgctgtcg agtttacggc gacctctttc tcgatgtggc gcgtcatcgc
     9661 aaccggtgct gcgcaatcgg caaacttgtt gacaaagttg aagtgatggc atacacacaa
     9721 caagagagaa gaggtccgat cggtccagat cgaacagggg aggatcgcat gaggcatgtg
     9781 tgggtggctg ggcttgcgct catgagcgcg atggccgtca gcgggtgcgt aagtgacaag
     9841 aaatatcatg aggcgcttgc cgatgccgat gccaccaggg gagaactgga gcgcgcgcga
     9901 gcgcagaaaa atgccctgga gcaacaggtc aagaccttaa aggatttgaa cgcgaagttc
     9961 ggttccgaaa gccagatcgc ccgtgatgaa ctccagcgta tccagcacgg gcgcgataaa
    10021 gagcgggata ccatcgatgt caggaccaag gagctggaag acaaggtcaa gcagctgagc
    10081 gcgcaaaacc gcagcgtcaa gcaggagtat gaagaagctc gccgccacaa tgagcaattg
    10141 aagtcattgg tcgcgcggta tcagaaagaa atgaaagacc ggtcgcgttc cctgtcatcg
    10201 ggaatggagt cgccggcctt gaccgcgccg ctgggcggcg gcggtgcttc ttcgaaagct
    10261 gctcctgcgc cggccccatt tgaggcaccg gctgcgccgc tgctcaatat caacaaggcg
    10321 ccgtcctcgg atctcgtgct cacgctgggt attacacagg atgtggcaga ccgcatcgtc
    10381 agtaaccgtc cctacagagt gaagggcgaa ctcgtcgcca agaatgtggt gacgaaggat
    10441 gtgttcgacg acatcaaaga ccgcattacc gtcagcccct aagcaccagt gagcaggtcc
    10501 accgctggtg gatcacgtga catgaacggc cgtttgccat tctggcaaac ggccgttctc
    10561 tttcggttcg ctccgcgctc agaggcccca tcgccgccct ctgcctgaca tcgtcaaatt
    10621 gttttctgct agaatgcccg cagtacctgg gcgaagagga acgcctgttc atgcgccgtg
    10681 tcagtatcat tgtcggtgtg cttgcgctcg gcctggccat cgccggttac gtgtttttca
    10741 acggcgagcg gaaggttcct ctgcgctttc gcacggttcc cgtcgagcga ggatcagtcg
    10801 tctcgctggt caccgcaacc ggtaccatca atcccattac gacggtccag gttggcagtc
    10861 aggtctcggg tatgatcgag agcctgcatg ccgatttcaa ttccaaggtc aaggcgaacc
    10921 aggtcgtcgc acggatcgat cctttcccct accaggccag gcgtgatcaa gcggtcgcca
    10981 gccttgccaa tgccaaggcg gcgtttgaaa aggcgcggat cgatctggct cagcgtcggc
    11041 gcgaactgga tcgagcaaag tcgcttatcg gccagcagtt catttcgcag aatgaagtcg
    11101 atgttgccct gaccgcttcc gagggcgccg tcgcgcaact gaaagtgacg gaagccgccg
    11161 tgaagcaggc ggaagccatg ttacaagcgg ccgagctgga cctgaagtat acggtcatcc
    11221 gctcaccggt cgacggagta gtaatttccc gtctggtgga agtcggtcag cgtatctctg
    11281 ccagcttttc cattcccacc ctctttctga tcgccgagga tgtgaccaaa atgcaggtcg
    11341 ataccaatgt gagcgaggcc gacatcggcg gcattgtcga cggcaaagcg gcgtcattca
    11401 ccgtggatgc ctatcccgga gaagcctttc aagggcgggt tcgccaggtt cgcaatgcgc
    11461 ccatcaatat tcaaaacgtg gtgacctatg acgttgtcgt cgaattcgag aatcctgagt
    11521 ttcgtttgaa accaggtatg accgcgaatg tctccatcgt cgtcaacaag cgggagaatg
    11581 tgctgaaggt gccgaacgcg gcccttcgct tcgtaccccc caaggccgtt cgagatgaga
    11641 agggagaggc cgggccgagg agggatggcg agcgcgccgg cggcaggcct acgatcgcga
    11701 gtgggtctcc tgaatcaggg aaccggcaat ggggtgtctg gaggctggat ggtgcgggag
    11761 agttggagcg cgttccggtc gagatgggta tatcggatcg gtcctatgtc gagatcactg
    11821 ctgccggtat ccaggaaggc gaccaggttg tgaccggtat cgagtctcca cgcggtgagc
    11881 gcaagagcgg ggaactccca ccgggcttcg gtggcggcca acagcgcggc gggcggcggg
    11941 accgtgggat gtagccatgg tggaaacagt cgcgtgcgat cccgtttgtg agggcacgat
    12001 ggctcccttg atcgtctgcg aagacctctg gaaaatttat cgcatcggtg atgtcgaggt
    12061 gcaggcgctt cggggcatca atttgacaat cgaccgcggc gagtttgtcg cggtgatggg
    12121 cacgtcgggg tcgggcaagt cgacgctgat gaatctactg ggttgcctgg acaagccgtc
    12181 gcgaggccgg tatcggctcg acgggttgga tgtgagtacg gccaacccag accttctcgc
    12241 cgatcttcgc aaccggcaga tcgggttcgt gtttcagaat ttcaatctga ttccccggac
    12301 cagtgcattg gaaaatgcgc aattgccgct gttttatcgc ggtgtctcga tcaaggagca
    12361 acgccggcag gcggccgccg cgcttcagcg agtcgggttg gccgggcggg aacagcatta
    12421 tccggcccag ctttccggtg gtcagcagca gcgcgtcgcc attgcgaggg cattggtcgg
    12481 cgccccctca atcctgttcg cggacgaacc gaccggtaat ctcgataccg cgtcgagccg
    12541 tgaaattatg gatatcctcg aacatctcaa ccgggaggac gggatcacga tcattctcgt
    12601 gacccacgaa cccgatatcg ccgcgtatgc gtcccgcgaa ctcgtcatga aggacgggca
    12661 gatcgtgcag gatgtccggc gcgagcctca cctatctgcg gtcacgtagg gccagatgcc
    12721 ggcattcgtt ctgctcactg tcatgacggc gttgcggatt ctgagccgga accggctgcg
    12781 ggccggtctc accatgctcg gcatcgtcat cggcgtggga gcggtgatcg ccatggtgag
    12841 tatcgggcaa ggggccaggg cggcggttca ggcccaggtg gcaagtatgg ggacaaatgt
    12901 catcgtcatc atgcccgggt cgacgacggt cagtggtgtg cgaggcgcgc agggcggggc
    12961 ggtgacgttg acgatcaacg atgcggccga attgaaaaaa cggagtccgc tcctgtccga
    13021 caccgggtgg gcgaagcgcg atgtgatgca aatcgtcaac gggaatcgga actggaatgg
    13081 ccccgtcaat ggagtctccc cgagctacct gacgattcgc gactggtcgt tcacgagtgg
    13141 cgggcccttc acccagacgg atatggacag tgcggctcgc gtggcgttga tcggccagac
    13201 caccttggag aatttgtttg atcctggcga agaggcgatg ggagccacaa ttcgcatcaa
    13261 gaatgtgccg tttcaagtcg ttggagtatt ggctccgaag gggcagtcgg cccagggcac
    13321 ggatcaggat gacgtgattt tcattccctt cacgacggcg gagcggaggg tattcggcag
    13381 ccagtttctc ggatcggtcg gggcgctctt cgcgtccacc gaacagagcg gcgatctccc
    13441 cgaggccgtg gagcatattc gggagacgct acggctgagg cacaggctgg agccggatca
    13501 agcggatgat tttacgatca ggacgcaggt cgatatcggg aaggtgcagg aaagtaccag
    13561 tcaaacgctc acggtcatgt tgtttgccat cgcctccgtc tctcttctgg ttggcggtat
    13621 cggcattatg aacatcctgc tggtctcagt caccgagcgg acgaaggaga tcggggttcg
    13681 tatggccgtc ggtgcgaaac gcattcatat cctgacccag ttcctgatcg aagcgatgac
    13741 gctgagtcta ttcgggggcc tgatcggcgt gattgtgggc atcgtcggcg cacgactcac
    13801 gacggtgatt gccggttggc cgaccatgat ttcagtcgat gccgttctga cggcgtttct
    13861 gttttccttg gctatcgggc tctttttcgg actctacccc gccaataaag ctgcacgtct
    13921 caatcccatt gaagcgctcc gttacgaata ggcctgcatg ccgccagggc ttctccagcg
    13981 gtccgatctt ctcgatgagg ggagcacacg gatggggctg aggtcagtga taggctcgtt
    14041 gaaattcttc cgggattgtt cccaacaccg aacggacccc gcgcggaggg gcctcttcat
    14101 cctgattggc gaggtcgatg acgagcggga cgatatacgg ttctccggaa gggtcgtggg
    14161 tgatcgtgac gatgggttgg tggagtttgc ctgagttgat gacggagacg atggcacgtt
    14221 cgccggtcgt cagctggaca taactgtaaa cggggtaaat gcccatgact ttgacgaaga
    14281 gggagatcag ccggctttcg tatcggcctt cctgtccctc ctggtagagc cgctggagcg
    14341 attgatgcgg ggtcaggggc gaggctccgc caaagcccgt catcaattcg tcgtagcggt
    14401 ccgtcaccat cacgatccgg gtcatgtccg acgtgaacgc gccgccggtt tccgctggaa
    14461 agccgctgcc gttcaggtag gcatgatgtt ccgccacaat ctgttccacc ggtttggtga
    14521 atccgccccg gcgttccaag agaatggcgc ccgagcgcgc gtgagcttca taggtcagac
    14581 ggttttgttc agagactgtg gtcgaggtgt catgcacgcg ccggaggatg gcgggtggca
    14641 cctgcaggag cccgatatcg tgaagcagcg caccggtggc cagttcctgg atcgccatta
    14701 gattgtagtc agccgcatgg gcgaggatca tcgagaaact gcaggtggcc agtgcatgct
    14761 gactgagggg cgcattggcc tctcggcctt ggctgaacac catgaacaag gcagaagtat
    14821 tgagggcctg ggcgaccgcg ctgatggcat ggacggcatg gctggcctcc tccggatcga
    14881 ccacaccggt tttggcgatg cgggagaacg ccgaatggac ggattcctcg agtttggctc
    14941 gcgctgacct ggccgccagc aattcttgcg tcatggtggc aagagaccgg atctcgacgg
    15001 acttgcccat ctgcgaagtc gcaggttgcg cggcaacagg gagcggggct tgggacgggg
    15061 ccattggatc ttgaagatcg agtccgcgag agggatcgat gctcacctgt ttcacgcccg
    15121 cgcgccgcag cttctcgatc tgctcgtcag tgcgaatcag gaacgagtgg cgcatgaagg
    15181 gggaacgaaa ccaggcgacg tcgatcttgg cgacgtgcat gccgattcgg agcgcatcaa
    15241 ttgtaatggg tcggtatgtc atagcgcgtg ctgcccgccg gcgaggcgga attcaaacgg
    15301 gtgtcgcccc aaccatccta tcggtcttgc ccgttgtcga ttggcggctt ggggatcacc
    15361 gcgacctgtg ccttgagtga ggcgggaaaa aacggatcag gcgccgtcga cggcgccacc
    15421 gataaccgga tttccatgcg aggattgtcg cgatcctggt gcttatagag atgtgtttcg
    15481 accactcgat tgtcgttcag tcccagcccc tcacacaggg catcttgcgc gatcttgagc
    15541 ccgccgtcag tatcccgtcg cagcgcagag gcgaagaaaa aatggatcga gagagacagg
    15601 ctcgcctgtt gcaaggtctg tcttagagta gcacgatgag gcgattgggc aagcgcaacc
    15661 acaatttgtt gcccgacaaa gtctttatac gtacgtcctt tcgcggagag caggcgacgc
    15721 ccgttgaccg tggcgtactg atgattgatg cttggcggga cgggcaatgt gagggtgatg
    15781 gagtcgatgg tgacgctcaa gccagtacct gccggggtag ggaacgacag cgcatggcga
    15841 ttcctgttgg actgtgtggg aatgagcggt gctcgttcca tgggtacggc cggctcggac
    15901 agtgacctga aggacgtgga tcggatcgta acgggggatt gtcggggacg ggatgcgcgg
    15961 cgcaagggcc tgcctataga agattttgga tcttagccat atcctgctcg gcccggtctt
    16021 cgtttctggt gaggtagcgc cgccattcag acgggcccca gatttccaga cgatgataca
    16081 tgcccacgag gacgagttcc tgatcctcat ccatcgggac gagtttgcgg agtcggcttg
    16141 gaatcaacac ccggccggct ttatcgatgt cagaggcgcc ggcttgggcc atcacatggt
    16201 gcatgaacag gcgggtctgg tcctcgtcca gggtggctct cgtgcgttcc aggacctgtt
    16261 cccattcctt ggcggagtac atccagagcg tctgctcggt gttcttcagg aacatgacct
    16321 ggttaccctc ggcttggaat cgctcacgaa gcggagaggg gatcaggaaa cgtccctttt
    16381 catcgacctt gcagagatgt tctccggcga acatgggcgg cttcctccgt ttcaacgccg
    16441 atggtaggag acatggctga tcctgtcaag agaatattct tacagtgcgt gtcagtcaaa
    16501 atccgttccg tcgatccccg atgctggcgc ttgcccatcc tccatcgctg catcgaggtc
    16561 atcgcccaga tcctcgccca tttcctgccc catcttcttc atgaaccgcg ccatgctctg
    16621 gggatcgttt tcatcgagtc cgctgagatt gctcgggtcg gagagggcct caagccgtgc
    16681 ttcctccgat ttgggcgagg cgaatcgcga cagcagccgt tccatgtcgg cgctctgaca
    16741 atgggcgcaa gtctgctgcg gaacggagtt cgcgttgaga atcaggcgtg tggtccgttt
    16801 tccgcactgt cggcaacgat actcataaat cggcatgggt taggctcgct ggaattccgt
    16861 ttggttgaga gttgatggtg gcagccacgt gtccggcacc atccccgcat ccgcggaccg
    16921 cgccagggcg aggacggcga cctgtttggc tccggccgta aggaggactc tggcgcattc
    16981 gctcgcggtc gtgccggtgg tgaacacgtc gtccaccaga agaatgcgtg ctccggtgat
    17041 ctccttcggc tggcgtatgg cgaacgcctt gcggaggttc tgtagacgcg ccgcgcgcgg
    17101 cagcgaggtc tgcggggcgg tatcgacggt acgaatcagg ttccgatagg ataccggtag
    17161 tctgcgggcg gaaccgatgc ggtcggcgat gagaagcgac tgattgaact cccgctgccg
    17221 gagtcgagtg gggtgcagag ggatcggcat caggacatcg acatccaggc gatcgggaat
    17281 cgcctggagg agtaacacgc ctaatgcgtc cgccagcgcc accttccctc ggtatttgaa
    17341 gagggcaatg gcatcctgga gcggagaaca gtaggcatag ggagcccaga cctgggagta
    17401 atagggttcg cgggtacgac agtcctggca ttcgtgcgtc gggctgtagg tgagggccga
    17461 gggtgaagcg aacgggcgat gacaccgtgg acaggaaggc ccgcgcaatg gtcgaatcag
    17521 ctcccagcac cggcggcaga aaaagggcac cggatcatca gcgaggggtt gcgcacaggt
    17581 ggtgcaatcc atcggaagca gcagccgcga ggcctgcctg agaaagtcca ttatcattgt
    17641 cgactacgta tcgttgacgt gaagcccttc agcctggtgg gattgtctaa ggaacgctcc
    17701 agacctgtca aggttgtggg ctgtaaaggg ggtgtgatat agcaccattc ggtctgcatg
    17761 cgggcgattt caccattgga cagggttggc cggctgacga tgcttggaac atcccacgat
    17821 cattcaccgt ccagtcctgc tgatatgagc ggttctgcgg ccccactcgt gagcgtacac
    17881 aacctctcga aagaatatcg ccgtggcgga acggtcttgt gcgctctgca gggcgtcacg
    17941 cttgaggtaa aggccggaga gttctgcgcg tttgtggggc ccagcggctg cgggaaaagc
    18001 acgttactca acttgatcgg aggcctggat caacccacct cgggaaccat catgatcgaa
    18061 ggtcgggccg tcaccgatgc gagcgcgacg gaatggaccc gtctccggcg cgagttgatt
    18121 ggcattgtgt ttcaagcctt tcatttgatt cccggcctta cggtcgcgga gaatgtggcg
    18181 ttgcccatgt tactcaaagg agagcaaggc cgcgccgtca cggatcggat cgatgaagtc
    18241 ctgcaggcgg tggggatgga ggcacgccgt tctcatcgac ccagtgaatt gtccggtggc
    18301 gaacagcagc gggtggccat cgcccgcgcg atcgtgcatc ggccccgtct gattttggcg
    18361 gatgagccga cgggaaattt ggactccaag caaggcgccg atatcgtgaa cctcttccgg
    18421 acccttcctc aacggttcgg ccattccgtg ttgttggtga cccacagcga cagcgcagca
    18481 gaagctgcag attatgtctg gcacatgcga gatgggcgtt tggtcaatcg cattgcacac
    18541 tgattccggg agggtgacgg atgcttactg ttcctaacgc tggccctgtt cgtgatgcag
    18601 aagagaaagg atcggtatga gtctcagcat gaccattgcg ggcgtcacgt ttccgagctg
    18661 tttgatgaat gcgtccgggg cgctctgtat gacgcgggag gaattgctgg ctttgggtcg
    18721 ctcgcgggcc ggcgccatcg tgaccaagtc gatgacggtg gaaccgcggg gcggaaatcc
    18781 tgaaccgcgt tattacggat ttcccggcgg gtcgattaat tccatgggac ttccgaatct
    18841 cggctatctg gcctatgcgg acctcattcc ggagctgaag cagttcggca agcctgtgat
    18901 tgccagtatt gccggtctgt gcgaagacga ctttctcacc atggcgcgcg tgatcaatca
    18961 cgcaaaaccc gatctcatcg aggtgaacct gtcttgtccg aacatccccg gcaagccaca
    19021 gatcgcctat gacccggtcg attccgaacg gcttctgaag cgggtgcggc cgctcatcac
    19081 ggtgccgatg ggcgtcaagc tgccgcccta ttttgatccg gctcaccatg cggtgatggc
    19141 ggaggtgatc ggccgttgcg gagtggacta tttgaatttg atcaattccg tcggcaacgg
    19201 cctcgtgatc gacccgaagc gtgaaacgcc cgtcatcaaa cccaaggggg ggttcggcgg
    19261 attgggaggt tcgttgatca agcctgttgc cttggccaat gtgcgagcat tctggaagct
    19321 gctcggcggg cgcattccga tcatcgggac cggcggagtc gtgcaggggc tcgatgcgtt
    19381 cgagcatgtc ctctgcgggg cgtcagccgt tcaggtgggc acggtgttgg tggaagaagg
    19441 tgtcggtgtt ttcgaacgcc tggagcgtga actggaagcc gaactgtctt ctcgcggagc
    19501 gcgacgggtg gaggactatc ggggcaggtt aaaagagtta taacgcgtgg cgcggagatt
    19561 ccacgccacg cggtcttcgg tcggattatc taaaggcaaa gttcttcggc aggaaacctt
    19621 gctgcagcgc ctggcggagc agttgggcca cgttgcggac cttcaatcgc cgcatcaagt
    19681 taaaccgatg cacttcaacc gttcgcacgc tgatatccag acggcgggcg atgtcccggt
    19741 tggtgtttcc ttgcgacacc agtttaagaa tttccttttg tcggggagtg agtccgtctg
    19801 agcgccttgt gcgtccggga gacgccttgg ccaacttcac gggctttcgt ttcttcgctg
    19861 cgggagcagc tttggtggtc ttcgaggatt tttttgcttg aggtcgtttg cgtagtgcca
    19921 taaagatttt gtaccccaat ttctactgaa agtaatctta acatacctgg gtaaatctgt
    19981 aaaaacaatt actagactgt aggacgaacg cgcgtacgta tgtcgttgtt tcactcttct
    20041 ttactgagag tctgtgcgag cctggggtgg acacatttgt ccacccatcc ggggcggaca
    20101 gcgttgacca tgttcggtgt cgggcttggc gtggcggcca ctattgccgt ccaaacggcc
    20161 aacgtggaag tcctgcgttc cttcgaggaa tcagtcttga cggtagccgg cccggtcacg
    20221 ctggaggtgt cggccgggga aaccggcttg gacgaacgtc tgatctccac cgtacggacg
    20281 gtccccggtg tcttgtcggc tcgaccggtg ctggaggtcg gggtgcgggt cacagacggc
    20341 cggtcccccc ggcagtcgtt ttcgatcctt ggcctggacc tattggaaga gttgagcccg
    20401 gagggaggac gtgtcgtttc tccagcgaat cgaggggagg gggctggtga gcgaatgcca
    20461 ctggaagacc tcctggcaag aaatggcctt ctgatcgggc acgccctagc gtcggagttg
    20521 agtctcgcgc tacgggctca gttggcgctg cagagcgggg atcagcacgt gtcggtgtcg
    20581 attatcggag tcatgggccg gcgtcctggt gggccttcgc cctgggaccg catggcggtg
    20641 atggatattg cggcagtgca gcggacgttc ggactgatcg gccgccttga tcgtatcgat
    20701 gtggtgacga cgcccacttc gtcggttgag caggtggcgg aggccatcgg gcgggttttg
    20761 cccccggcta ccaccgtacg tcgcccgatt cagcgcagcc agcaggttga atcgatggta
    20821 ggggcctttc aactgaatct ctccgtgttg agcatggtgg ggctgttggt cgggatcttc
    20881 ctgatctaca acacggtctc ctttacggtg gcgcagcgac gtcgggaggt ggggattctc
    20941 cgcgccatcg gcatgtcgga gccgatggtc gtcgctgtgt ttctggcgga agccggagtg
    21001 ttcggtgtcg tgggcgggct gctcggaggc atgctcggcc tgttgttggg cgatgtgctt
    21061 gtcgcgctgg tcggccggac gatccatgat ctgtatacac cgctgtccca ttcgacagga
    21121 actgttggtt ttcctgtcgg gtctggccgg ttattggtcg aagcggtatt gatcgggagc
    21181 ggagtgtcag ttctgggcgc gcttggtccg agcgtcgatg ccggacgtac gattgtggtc
    21241 gcggctctgg caccgggtga gtacgacgtc gctcaacgtg ttcgcgcggc gtcactgggg
    21301 gtggcgggtg gagtgctgtt actcatcgct ctgggatgcg cattggccgg cccggtgggt
    21361 ggcgtaccga tcttcgggta cgtggcaacc ttttgtcttc ttgccggcct gtcctgcctc
    21421 gtccccatcc tgttgcagag cctctgccgt acccgggagt taacgacgct ttcgcaggcg
    21481 ccgacgttgg gcggggccat tcggcacatt gcccgagagc agaccagccg gggcatggga
    21541 agaaatgccg tcacggtgtc ggcctttctc gtgggcgtcg cgatcatggt cggcgtgctg
    21601 gtgatgatcc gcagctttcg cgacacggtt gaaatctgga tcgatcagac cgtcatggcg
    21661 gactttattg tcgctcctgc cggatggccg cacgccgtgc gcagcgggtc gtccagtccg
    21721 gtactgcctg gtgcctggcg ggctcggttg gccaatgctc ctctggtctc ggcggtagac
    21781 atgtaccgtg acctgcgtat cgatctgcag ggccggccgg tctcgctggt ttcgagagac
    21841 ctggcactcc atgcggcacg gagccggtat cttttcgtca cgggggagtc cgccccaatt
    21901 ttggctcgtg ccgcggccgg ggaaggcgcg attctttcgg aagtcgtggc gaatcatttg
    21961 gcgctgggca aaggggatcg attgtccatc gtgaccccaa agggcgaaca gtccctggag
    22021 gtgctgggag tcttttatga ttatgccacc gatggcggaa agatcgtcat tgatcgatcc
    22081 ttgtatcaac aatggtggcc tgatgacggc gtgaccgtgt ttcccgtcta tgtcgaatcg
    22141 ggggccgata tggggaaggc aagaatggcg attctcggca ccctcgctca ggcgtcgcag
    22201 ggaaatctca tgccgaccgt attgagtaat gcagaattgc gccaggagat tcttcgaatt
    22261 ttcgaccgga cgtttacgtt gacctatgtg ctggaagcca tcgccgtgat tattgcgacg
    22321 ttaggtatca tcaacacgtt ggtcacgtcg gtggttgagc ggcgtcgtga attggccacg
    22381 ctccaggcct tgggcagtag ccgggggcag gtcacggcgt tgattttatg ggaggccggc
    22441 tatttgggat tgctggggac agggatgggt ttggtcggag ggggagccct ggcgctcatt
    22501 ctgatccggg tgatcaaccg gcagtcattc ggttggacca tccagatttc gtggcccatc
    22561 ggcctgatgg cggaggttgc cgccttggcc ctggtggcct cgctcgtcgc agggttctgg
    22621 ccggcccgct gggccgccag acaacccttg gtcgagggac tgcgttatga atgaaggccg
    22681 acagccggtt cggcggacga agtggcaccc tgtgtcggaa gcgcgtccac ttgaatgtcg
    22741 aatcccaggt cttcgatcat gcgccagact tctgcggccg gttgtcccgg agtggtgaga
    22801 tacccgttca caaacactga atcggccggg tagagtgcca atggctggag gctgcgtaag
    22861 ttgtgttctc gtccgccggc gatacgaagc tccgtccgcg ggtgaaggaa gcggaagagg
    22921 cagagggctt tcaggcaccg ctgcggcgtc agcggagtgc aatgctccag tggcgtgccg
    22981 gaggccggat gcagcatgtt gagcggaatc gaatcgggct tgacctcgcg cagggccagt
    23041 gccaaatcga tcaagtcctc atcgccttct cccatcccga cgatgccgcc tgaacagatt
    23101 tccagtccgg ccgtcctggc atgtcgaatg gtggcgagcc ggtcttgaaa ggtgtgcgtg
    23161 ctgcagattt ccgtgtgata ggcttcgctc gtattcaaat tgtgattgat gcgatccacg
    23221 ccggctgctt tgaggtcttt tgcctgccgt tcgttgagga gtcccaggga acagcagatc
    23281 tggatcggga cttcctgctt gatggatcgg acggctgagg cgatatcggc gatctcccga
    23341 tccagcgggc tgcgtccgct gatgacgatg cagtagcgtt gggctttcgc ggctgccgca
    23401 cggcgcgcgc cggtcaccat ttgatcttgc ggcaacaatc catatcgttc gatgggcgcc
    23461 gtggacacgg atgattgcga gcagtagccg cagtcttcct ggcaggcgcc gctcttggcg
    23521 ttcaagagca tttgcaggcg caccgtctta ccgaagtacc gttcccggac cttaaaggcg
    23581 gcctgcagaa gctcgagcag gttggtgtcc ggtgtatgta gtacggtgag gcattcctct
    23641 cgggtgagta attcgtcccg caaggccttg tcggccagga tcgtgaaatc agtcatgcac
    23701 gctactcctc gaaaaaacag gcaagccccg ctccttcagc aatgcctccg tgagcaaagg
    23761 atctgtctgt aacatgaaaa aagggcggtg gaaaccctac acggtttcaa acggagatgc
    23821 aatgccttga taggggacgg caggcagccg ggcagcctgt ccgtcctgat cggcaccgga
    23881 tttggtgctg tctctccagg gaagggcgac gtaggtattc cagcggccgc accgccggca
    23941 ccggccggac cattcggcca actcatggtg gcactgggtg cattggtagg gcaccacgac
    24001 tcgctttttg aagcccaggg ctttcttgag ctcgcccacc gcttcgtcca tgtgttgctt
    24061 gcgcaggaac agattggcca tgatcttatg gtaatcaacg agctgttcct gcgtgccatc
    24121 cagcgttgaa agcacgtcgt acgcttcatc gaccatttcg agccggtaat acagcttgcc
    24181 caggtagaac tggatcacag cattgtgcgg atcctgttgc agagcttcct ggtagacacg
    24241 gatgatttca tccggttctc ccagctccag gaacagttcc tcgaggcggt gaaggatgat
    24301 gacgctgcgc gtcttggcgt aaatcttctt gagaatttcg accgcgttct tgattttgcc
    24361 ctcacggacc aagatttccc cgatgccgat gtaggccggc agaaaggtgc gatctttctt
    24421 aatcgcgcct cggaagtacc ggcgggcctt gtccgggtgc cctcgttcca agagttggcg
    24481 gccgacttcg tacatacagc ccaataacag attggcctcg gcgcgtcgct cgctttccgg
    24541 caggttagcc ttgaccaacc ggtgttgaat ctccagcgcg tcgctccatt tttcgagcct
    24601 gatttgcaga tcgcgcttgc ggatcagggc cattagattg tccggctcga tccgcagaat
    24661 attctggagt gtctgaagtg cctcttcata gcgacgggcg ccttcaagat ctttggctaa
    24721 ttcgagcagc acctccacat ttcgttcttc aatgcgatag gcctgctggt ggagccggat
    24781 ggcctccgta aagttgcttt cagagcgata gatatttccg agccagagca gcgaatcggt
    24841 ccggttcgga tcaatgacca gggcccgctc aaacagactc accgcatcgg ctgtacgctt
    24901 ggacatgaag gcgtgcgtgc catcccggtg gagcgcgtcc actttctctt tacggcggac
    24961 gaggcgattg gtgcgccaat tcatgaagac gtgggaggtt tctttgatcg tcacgatcag
    25021 tgcaaccagc gtggcgccca gggccatcga gaatagcacc aaggagacgg ggctgagctc
    25081 gaagatcgcg tcgggactgg tccggacggt gatcgtgccc gggttcagtt cgcggaaata
    25141 gctgtagaga aagatgccga cactgaccag gaagatcgtg gagaggagcc gaaacatgct
    25201 agacccgttt ccgggtgcga gcgggacggg ccttcgttcg tgcgggggga gtcaccttca
    25261 cggcgcgctc ttccggcagc ttgacctgtc ctgcgtcgcc ccacagtcgt tcgagggcgt
    25321 agtgttcgcg aatatccttt tggaagatgt ggacgatgac gtcaccaaag tccatgacga
    25381 cccacttggc agacgacgcg ccctcgatac cgagcggcgg atgcccctgg gccttgagca
    25441 catcgccgac atggtccgcg atcgcccgtg cttgacgctc cgactctccg gagccgatga
    25501 ctaaatagtc ggcgatagag gtcagcttgg cgatatgcag gatcaggaca tcggtggctt
    25561 tcttgtccag aatggcggcc gccaccgcga gagctttggc ttttgtttca gaaacgctca
    25621 ctgtcctccc tgtataaccc ctcgcgaagt atataggatt cgaccagggg gggcaacaga
    25681 tttgccagtg cacggccatc cgcgacgcgt gctctgattt ccgaggcaga gctctcgcag
    25741 ggcggcagcc tcaggaaagt cacggtccgg ccggttttca ggggcacgtc cgctcgctcc
    25801 agccggcctg tatccagagc gagcaactcc tccttgggca cttcgggaaa gagaggcagc
    25861 gaggccacgg tcagaaacgt tgtggcgggc ctggaaatta cgacgaaatg acacaggnnn
    25921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc cacggtcaga aacgttgtgg
    26041 cgggcctgga aattacgacg aaatgacaca ggctaagaag ctgtgcggca tccttccagg
    26101 aaggcaggtc cagaaaggca tcgaggccaa tgatgaagaa aatctccgtg tcggacccgt
    26161 attgctcttg aagggcacgg accgtgtcga tggaatagga tttgccggat cggcagattt
    26221 cgatatcggt aagggtgaag gcaggctcgt cctgaatagc caattgcacc atgcggtagc
    26281 gatgctgtgc ggacgccaag gtgtctggtt gtttgtgcgg aggatctccg gtcgggatga
    26341 acaagacttg atccagacac agcagacgcc gcgccgactg cgcaatcgag agatggcagc
    26401 ggtgaatcgg attaaaactg ccgcccaaaa ctcccagacg cataggaggc taccttcacg
    26461 cacgattgtg caggtctcgg atggacaggc ccccgtcgaa cgacgccctg catcggcggc
    26521 gtctggcggc cgctacaaga gcaccaggtt gtcccggtga atgacctctt catactcttg
    26581 gagaccgggg atcttttgga tatcggcggt cttgaggccc ttgatgcggg tcaggatcgt
    26641 ggaggaaaaa ttaaccaggc ctttggcgca ctctttgccg tcaggtccgg cgcaggtgac
    26701 cggatcccca gcttcgaact ggccggtgat gtcgcgaatg cctgaggcca gaaggctttt
    26761 gccgtttcgc accagtgcat ccaccgcgcc ttgatccaat tgcacatgtc cgcggggccg
    26821 caacgtgaat gcaatccaat gtttgcggct gttcatgcgt cgctcccggc cgagaaacag
    26881 gctgcccccc ggttgcccgg acagcacatc cggaagcaga cccgtgcgag tcccgttcag
    26941 gatcaaggtc gcgaccccgt attcgcccac tttcttagcg gcgcggacct tcgtggccat
    27001 gccgccggtt ccttcgaagg tggaggacat gcccgcccgt tgttcaatgt cggccgtgat
    27061 ctcctgaatc aggggaatca aggtggcttg taaatctttg cgagggtcgg cggtgaacag
    27121 tccatccacg tctgacagaa tgatcagcag gtcggcgtct acgaggtggg cgacctgtgc
    27181 tgccagcgta tcgttgtctc ctacgcggat ctcttcgacg gcaaccgtat cgttctcatt
    27241 gatgatgggg atgacgccga atttgatcag cgtggtgagg gtgtgccggg cgttcaaaaa
    27301 ccggcggcga tccgcgaggt cttcatgggt cagcagcact tgggccacgc gaagcgagag
    27361 gcgctcgaac gccttctcgt aggcccacat cagcaggctc tgaccgaccg ccgcggccgc
    27421 ctgtttgacc ggcaggcttt tggggtactc acgtagctcc agttttttga tgcccgagac
    27481 gacggcaccg gacgagacga ccagcacttc tcgcccctgt gcgcgcacgt gggcaatttc
    27541 ggaggcgagt cgttcgatgc gctcggtgct caggccggat tcgcgtgagg cgaccagact
    27601 gcttccgatc ttgatgacga cacgtctggc ttgctttagg agttcgtctc gcacggggtc
    27661 ttccggagca gttccacctg ttggcccatg tagagtatgc actcgtccag accttctcgc
    27721 gtggctgccg aaatcgcaaa aaatggaatc ttgcgccgct ggcaatattt ccgaagttgt
    27781 tcgaggcgct cgccttcgcc tttgatgtcg agcttcgtgc ctactacggc aaagggacgc
    27841 gcggcgagtg cctcatcgta ggcggagagt tcatgacgca tgaccttgag gctcgccact
    27901 ggctcctccg tggcccactc tgaaatatcg atgacatgga tcagcaggct ggtgcgctcg
    27961 atgtgccgga ggaactgaaa gccgaggcct ttcccttcat gggcgccttc aatcagtccg
    28021 ggaatgtcgg caatgacgaa gctgtgttcg cccgtcgagc ggacgacacc caggttcggt
    28081 gtaagggttg taaagggata gtcagcgatc ttgggacgcg ccgcagagac ggcggcgatg
    28141 agcgtcgatt ttccggcgtt cgggtagccg actaatccga catccgcgag gagtttcaag
    28201 tcgaggcgca ggaggcgttc ttcgcccggt gtgcccggct cgaactgagt cggaacgcgg
    28261 ttggttgagg tggcgaatcg cgagttgccg cggcctccca gtcctccctt ggcgatgacg
    28321 caactctcgc catccgcggt caggtccgcc aaaagttcct gcgtatttgc atcgagcacg
    28381 atcgtgccga ccgggacagg tatccgcacg tctgcgccgc gtcgaccctg gcagttgctg
    28441 ccgccgccga cctcgccgtt ttctgcttcg taatgttttt ggtatcgaag atccagcagg
    28501 gtgctgaggc gagtcgtggc ctcgacaatg acgctgcctc cgtccccgcc gtccccgccg
    28561 tcggggccgc cgcgcgggac aaacttttcc cgacggaagc tgcaggctcc gttgccgccg
    28621 tgcccggcct ttaccaggat ttggacttga tcgacaaatg tagacatcgc agcgagcccc
    28681 cacccggcgt catttattca ggaatcacag tatagcgtat ccgccggctc atgggggatg
    28741 ctgcgtaagc gaaggggcgc tgttccgcgg aacaagactg aagctaagga gggatcaggc
    28801 cttcgcggta actggataga cgctgacctt ttgccgtccg cggccacctt cgaactttac
    28861 gacaccggac acgtaggcat agagggtgtg atcgcgtcca aggccgacgt tgaggccggg
    28921 gaagaatttc gttccccgtt ggcgcacaat gatggagccg gccttgatgg tctctccgcc
    28981 gtaggctttt acgccgaggt attgtgggtt actgtcacgg ccgtttcttg tcgaaccgcc
    29041 gcctttattt gttgccatgg caaactcccc ttcgagtcgt tacttcgatg cgatctcagt
    29101 gacccgcagc tgcgtaaagc cctgccggtg gcctctggtt cggcgataat ttttgcgacg
    29161 ttgcttcttg aaaaccgtga tggacctggt gcgtccctgg cggatgattt ccgcggtgac
    29221 tcgcgcgccg gcaacgaccg gctggccgat gaccagtcca ctgtcgccat tcagcaatcg
    29281 aaccttgtcg atctgcacgg aatggccgac atcgcccggg agcgactcca cttggagtac
    29341 ggtccctgtt tccactcgat actgttttcc acctgtttcg ataatagcgt acatgtgcgt
    29401 ccttctcctc gtcagcagaa cgtgggtgtt tatcacagcc tcggcgagag tgtcaagaat
    29461 tagtcctggt gttgcgcgaa accattcctt gtcgtgcggc acgaagaagt ggagtcgtac
    29521 ggtcggaggc aacaaaacta cggcccggga gcgtggcggc tgttatcttg acgctcatcg
    29581 cgagccgctg gtattatccc gcgcatgtta caaaccagcg aaaaaatatg gatggatggc
    29641 aagtttgtgg cgtgggccga tgcacaggtt catgtgttga cccactcact tcattatgga
    29701 ttagcggcct ttgaaggcat ccgctgctat aaaggcacca gcgggtcggc cattttcagg
    29761 ctacccgagc atgtggaccg cctatttgag tcggcgcaca tcggtctgat cgagattccc
    29821 tatgaccgaa agcaaatgac cgacgccatt atcgaaacgg tcagagtcaa tcggctagag
    29881 gcttgctaca ttcgcccgct cgtgtatatc ggctatggcg ccatgggact gtatccggga
    29941 gaaaatccca tcaaggtcag tatcgctgcc tggaaatggg ggacttatct gggggatgag
    30001 gcgctgcaga agggcattcg tgccagagtg tcttcattta cacgacacca tgtgaacgtg
    30061 tccatgacgc gtggcaaaat ttccggctac tacgtgaact cgattctcgc caagcgggag
    30121 gtcaaagccg acgggtatga tgaagcgatt atgctcgatc ccgaaggtta tgtggctgag
    30181 gggacgggag aaaatgtctt catcgtgcga cgcggtgtgt tgaagacgac tccgttaacc
    30241 tcgattctgg aggggattac gcgcaattcc atcattcagc tggcgaaaga gcgcaacatt
    30301 cccgtggttg aggaacgatt cacccgtgat gaaatgtatg tggccgagga agtttttgtg
    30361 acggggacgg ctgcagaact cacgccggtg accgaaattg atcagcggcg tatcgggacc
    30421 ggaaaaccag ggacgatcac gcagacctta cagaaagcgt tttttgatgt cgtgcgcggg
    30481 accgatgcgg ctcacagtca gtggctgact cccgtttagc aagtctcggt aacctcacct
    30541 gatctcttag tcgagcattc atcacattga tgtgctgtgg cgcgaacgct gcactggcct
    30601 tcgaactcct gcactcagag tgcttctcca ttccactact agcgtagtgc ccgcgcgagc
    30661 aatcataggg gtctcacttt ttagtcagag cgagaggaaa cgattcggct cgttgatcga
    30721 aacctgcgtg gaatcgaagg ctagctggaa cagtaggtct tagtgggagc tatcggtgga
    30781 ggaatgcgag tctttcgccg gtggcgtcgc ggtttctgac ggtgtcgtac tgggagtggt
    30841 tgccgcaggt ttcttgttca gatcaatgac cgtcgaggaa aagtttcgat ccttcgcaag
    30901 aatggccaga gacaatgacg tgaacatgaa gacaaacgcg gtgatgaccg tgaacttgct
    30961 caaaaagttg gcgggccctc ggctgccgaa cacggtttgg ctggatccac caaaggccgc
    31021 tccgatttca gcgcccttac cggattgcag cagaatggct ccaatcataa ggaggcagac
    31081 gatgacatga atgatgatga gcaatgtgta catgtttgag tctttaggcc gtggtaggtt
    31141 ttgacccgat ggcgactttg gcgagtgtag caaaagagac cggatctaaa caagccccgc
    31201 cgactaatgc gccgtcgatt tcttcggaag ccagaaattc ggcgatattg tgcggagtga
    31261 cgctgcctcc atacagaatc cgaacacgcg cccccgcttc agctccgctc agttcattga
    31321 tggtttggcg gatcaatcga tggaccggca ccgcctgttc cggcgatgcg gctcgccccg
    31381 ttccaatggc ccacactggt tcataggcga tgaccatcga ctcaagatgt tgcccctcga
    31441 ttccagccag tcctgcgcga acctgtcgtt ggattacttg gtctgtttga ttggtatcgc
    31501 gatcgtgaag tgtttcgccc acacagagaa ccggttggat gccgttacgc aaagcagcca
    31561 gaaccttctt gttggtccag ctgtcttgct cgccgaagta ctggcggcgt tccgaatggc
    31621 ccaccaggac aaattgacag ccgagatcct tcagcatggc tcccgacact tctcccgtga
    31681 aggccccctt atcttcccaa tacaggtcct gtgcgccaag cagaaaatcc ggtgaggaac
    31741 cgagcgcctc atgggctgca tgcagcgcgg taaagggcgg tgcaaaaccg acctggattc
    31801 cttcagacct aggcaactcc tgggtgaggc gacgaacgaa cgcccccgct tcggtggcgg
    31861 tcttattcat tttccagtta ccgatgagga agcgggttct cacgaagcct ctagtgggag
    31921 aatggagaaa gggtgtcgtt acgctgcacg attggggagg gcggcaagtc ccggtaaggt
    31981 tttcccttcg agcagctcca gggctgcgcc accaccggtc gagataaacg aaatgctttc
    32041 ggactctccg gcgcggtgaa tagccagggc tgtttcgcct cctccgacaa tggtcagcgc
    32101 gtaggcgttg gccacggagt gagccatggc gagggtgcct ctcgcgaagg cgtcgacttc
    32161 gaacatgccc atggggccat tccacagaat ggttttcgca tcctgcacgg cttccgagaa
    32221 caattttacg gaggcgggac cgatgtccag gccataccat cccttgggaa tttcctgaac
    32281 cggaacgatt ttcgtttcgg ctccgggctc ccggccggcc gcaacgacgc agtccacggg
    32341 aagatagaat ttgacgccac tggcaaaggc gtggtcctgc acccctttgg caaagtcgag
    32401 catgtcgttt tcaacgagtg actggccgat ctcaagaccc atggctttga ggaacgtgaa
    32461 cgccatgcct ccgccgatga tgaccttgtc gactttcttg ccgagattct cgatcacgcc
    32521 gatttttccg gataccttgg cgccgcctaa gatggctacg aacggacgca ccggattttc
    32581 gacggctccc tccaggtatt cgatttcttt cttaaggaga tacccggctg ccgcttcggg
    32641 aatatatttg gtgatgccga ccgtggatgc atgggctcga tgggcggcac cgaacgcgtc
    32701 attgatatac acatcagcga gtgcggccag cgccttggag aatccttcgt cgttcttttc
    32761 ttcttcggga tgaaagcgga ggttttccaa taacatcaca tcgccaggct gcatcttggc
    32821 aaccaggcct tcaacggcgg agccgatgca atccggcgca aaaatcactt ctttgccgag
    32881 caggcgctgg aggcgtttgg ccactggagc caggctgaac ttcggatcga atttgccttt
    32941 cggacggccg aggtgggagc acaaaatgac cttggcgccc tcgtcgacgg cacgattgat
    33001 ggtgggaagg gtcgatcgaa tcctcgtgtc gtcggtgatc tgcaaggaat catcaagggg
    33061 tacgttgtag tcggcgcgaa taatgacgcg ctttccgcga agttgcaggt cctcgataat
    33121 ccgtttgcga atattcatgg cagatcctca ctcctcctct ggtgtgtccg cgccgggcgc
    33181 tggcagctcc ggccggtgct gcacgtcaga aaagcgtgtc gtgcgtgacg cctgttacgc
    33241 cgattttgca acaatatact tgatcaggtc ccgcacgcgg caggaatagc cccactcgtt
    33301 atcgtaccaa gcggtgactt tgacgagccg tttatcgatg acggcggtca gcggagcgtc
    33361 gacaatggca gagtgggcat catccttgag gtcgatggag acgatgggtg cttccgagta
    33421 ggccagcaca tttttcatgg gcccttcagc agccttcttg aatgcggcat ttacgccggc
    33481 cacatcgcaa tccttctccg tttcgacggt gaggtccacc agcgagacat tgggggtggg
    33541 aacgcgaatg gccaatccgt cgagctttcc tttcagttgc ggaataacca ggtgcagggc
    33601 cttcgccgct ccggtgctgg tgggaatcat cgacatccct gcagcacgcg cccgccgcaa
    33661 atctttgtgg ggcagatcga ggagctgctg gtcgttcgtg taagagtgga tcgtcgtcat
    33721 gacgccgtgt ttaatgccga agttttccaa caagactttt gccaccgggg ccagacagtt
    33781 cgtggtgcag gaggcattgg agacgatatg gtgagctttg gcgtcgaaga cctgctcatt
    33841 tactccaagg accaccgtgg catcaggatc tttcgcgggg gcggaaatga tgaccgtttt
    33901 agctccggcc gacaggtgct ttcccgcgcc ttcgcggtcg gtaaaatggc cggtcgattc
    33961 gacgacgata tccacgccga gagctttcca tgggagctct ttggggtccc ggaccgcgag
    34021 aaccttgatc gcgcggccgt caatgatgag ctggtcgtct ttggcctcga cggaagcgtc
    34081 cagcgtgcca tgaaccgagt catacttgag caaataggcc aatgttttgg catcggtgag
    34141 atcgttgatg gcgacaaatt cgaggctggg atctcccagg gaagcgcgaa gaacgttgcg
    34201 gccgatgcgt ccgaatccat tgatgccgat gcgtgtggtc atggggctaa atcctcaaga
    34261 attaaacggg ttgaacagag taagtctggg gcgatagtac tgaccgactt tctgcgtgtc
    34321 aagcatacaa aaagagggaa ggccggtgga tattggcgaa agggcctcag ggaaatgatg
    34381 gggtgttggc cgtcttgttg tggacgtatg gggtccggta gagtctcatg cctgagcgtt
    34441 catcgagatg gggcgtgatg caatctgaaa agctgcagtt cgaagcgtgc aaggtcttgg
    34501 agtgggccaa actgttggat ctgttggccg gctacgccca gtcggaggtt ggggcggagc
    34561 aatgccggtc catggaattg gagcgggacg tggcgcaggc tcggctgcgc caggaagaaa
    34621 cctccgacat gttgcgcctg cgtgccagcg tcgacccttt tcctgtcctg cgattccctg
    34681 acgtgacgga gtggttgaag cgagtggtga aaggcgcctg tttggaggcg catgagctac
    34741 gagacatcgc gctggtgttg gaattggtcg tggatgtcca gcgatacctg ctgcgtcatc
    34801 agtccgatgc tccgacggtc ggcgccatgg ccgagcagtt gggaccggtg atggaatacc
    34861 gccggctaac ggtcgcgcta aacggggcca tcgaccccga tgggtccatt cgtgaatcag
    34921 ccagccctga gttacaccgg ttgatccaac gggccaacga tttcaaacaa cagatgcgac
    34981 atcggctcga gcaaattctg cattcgcggc gctatgaaga agtgttgcaa gaacgatatt
    35041 ttgcacagcg ggaagggcgt tatgtggtgc cgatcaaggc cgagatgcaa ggccgcatgc
    35101 ccggtatcgt gcacgacgtc tcatccagcg gtgccacact gtttctggag cctcgggaac
    35161 tggtggatct caataactcc atcaaggtcg tcgatctgga tatcgaacgg gaggtgcgca
    35221 ggattcttcg ggaactctcg gcgctggtgg cccctcatca accggcgctc gccggcagtg
    35281 tgactctgct cggtcggctg gatgcgattt cggcaaaggc aggcctgagt caacgtctgc
    35341 aaggttcccc gccgcggctc aatgaccagg gacgcattct ccttacgcag gcgcggcacc
    35401 cgtttctggt ggtgagcaaa gatcaggtcg tgtcgaatga tctcgcgttc gatgaaaccg
    35461 tacgggtact gatcatttcc ggcccgaata cgggcgggaa gacggtcatc ttgaaattac
    35521 tgggtctgtt ctctctgatg gtccgctgcg ggctgcacct tccctgtgcc cccgagtcgg
    35581 agatggcgat ctttccgtct gtgtatgcag acatcgggga tgcgcaagat ctggcgcgcg
    35641 acctgtccag tttttctgcc cacatcacac aaatggtgca gttactgaac gaagtgtctg
    35701 atggagcggg gagcgaacac gacagggagc cggtgcctcc cggacgagcg ctggtgttgt
    35761 tagatgagcc ggtcacctcc accgatccgg ccgaaggcgc agcgctcgcg agtgcactcc
    35821 tctgtcggct cgctgcggcc ggcgtgaaag tggtggccac cacacactac agtctcttga
    35881 aaggtctggc ccaggacagg ccgggcttcg ccaatgccag tgtggaattt aatctcgaca
    35941 cgctttcgcc gacctaccgt ctcattcaag ggcaaccagg cggctcggcc gcaatcgaaa
    36001 ttgccgggcg gctcgggatg gatcagtcgc tgctggagga tgcccgcgca cggctgcagg
    36061 gagacaaccg gttgctggag acactgttga gcgatttgca ggacaaacaa cgacgactca
    36121 atgacgacct ggtggcggcc acggcggcca ggcagggtgc tgagcaggct tctcgcgagg
    36181 cgcaggagtt gctcacgttc ctgcaggaat ccgagcggga tgagcggcag gggttgaaga
    36241 aaaagttgtc tcaggagttt caacgggccc gcgccgcggt gcaggcgacg atggacgatc
    36301 tcaagcgcga tcaaaagctg ctcaaggcca aggaggccaa agcccggctg gtcgaattgg
    36361 aagagtcagt ccggcgagag gccggagact ccgaagaacc gattccgatt gatcagctga
    36421 ccatcggaga tgcggtcgaa gtgatcgggc tggggatgac aggtacgttg ctggaaaatc
    36481 cccagggcaa aaaacgcgtg cgtctgaaag tcggcgaggg cgaaatcctg gccaatgtgg
    36541 cgagcctggc gggagtctcc aaggctcgcc agccacaagc gccagccgcg gtcaaaacga
    36601 gcgttgtctc accgaggcga accagccaga ccttggcgcc tgaagacatc caggagacgc
    36661 tggatgtccg tggtcaggca gccgacgatg cgttggatgt ggtggtcgcc ggtctcgatc
    36721 gcgccattct cggcggtagc ccgttcgtac gcatcatcca tgggcacggg accggccggt
    36781 tgagaagcgc cttgcgagac tacctgaaag actcaccgta cgtggtggcg tttcgagggg
    36841 gcgatcgcgc agagggaggc gacggcgtca cgattgtgca gctgcggtga gatcccgccg
    36901 agacccgctc aaaaacaccc gtagcccctt tcgctctttc tcgttctcca gtacgatagc
    36961 ccgcctgtga catgatcggg ggcggacctg tcttgttcat gccattttct ccacgaacca
    37021 aaaaaatcct ttcagcgatt gccgtcggag ttgcggtcta tgccctggtg accgggctcc
    37081 gcatgacgcg atggttcgag gtggtggaac tcaaggcctt cgaccatctc gtccggcgct
    37141 atgccgatcc tgccaaggcc gattcaagtc tcgtgctgct ggccatcgat gaatccagcc
    37201 tggaagcgtt cgggcgctgg ccctggccgc gtgaccggta cgggtatgtc gtgcggtatc
    37261 tcaaacgggc tggagccaag gcggtcgtct tcgatgtgat gtttttcgaa ccggacgata
    37321 atgccgaaga attcgatcaa tccttcgccg acgatgtgaa ggccgcgggg aatgtcttcc
    37381 tgccgatgtt gtttcaatcc gaagcggcgc cggttccagc cgggctgcag gaccatgcca
    37441 ccgtaaaggc cgaacgatcg gactcaggac cggtacaggc tgcagcgcac ccgggtgtga
    37501 aattgcccat tcctgtgttg gcgcaacagg cgcgtgggtt gggcgtcatc aatctgtctg
    37561 ccgacgccga cgggccgaca aggcgtcttc ccctgctggg gcaggtgcaa ggaaatctgg
    37621 tgccgcatct ctcgttggcg gtagcccgat acgtgttagg cggtagcgag ctcgcgatgc
    37681 gggacgaccg cttgcagatc ggcgacagca gcgttcctct ggatgcagac ggcaatctgt
    37741 tgatcaaatg gcatgggtcc ctggagcaga cctaccacgc caggagatac tcgatcggcc
    37801 gggtcttgca ggccttcgcg cagcaagaaa aaggggaacg tccggatctg gatgcgtccc
    37861 tgttcaaaga caagatcgta tttatcgccg ggacggcggc agggttatat gatctgcgag
    37921 tgacaccgtt ttcgtccgcc acgcctggcg tgctcatcca catggcggcg ctggacaacc
    37981 tgctgcatgg gcaggggctt caagcggcgc cgtcctggtt ttctctttcc atccttctgc
    38041 tgctttgcct tgtctcagcc ggtgccttca tgttgttccc gtcctatccg gtcaaattcg
    38101 gcgtcacgat cggcctcgct ctggcctact atgggttggt ggcgcatgcc ttcgcggggc
    38161 atgagcggtg gctggatctg gtgtttccgg aagtcgccct cgccttaacg ttcggcacgg
    38221 ccgcgacggt tgagtacgtc accgaaggca agcagcgccg gcttatgcgg gccgcgttcg
    38281 acaaatacat gtcgtctgaa gtggtcgaag aaatcatgcg gaatcccgcg gccattaagc
    38341 ttggtggcga gaagaaggag atttcgattc tcttctccga catcgccggt ttcacgacca
    38401 tctccgagaa aatgtcgccg gaggatcttg tcgcattgct gaaccggtac ttgtcggcga
    38461 tgacgaccat catcaagacg acgcatcgcg gcaatgtgaa caagtatctc ggcgatggaa
    38521 tcatggcgct cttcggcgcg cccctgaacg atccccaaca tgcgtcgctg gcctgttatg
    38581 ccgcgctgga ttgccaggtc gaattggccc ggcttcgtca ggtctggaag caggagggac
    38641 ttccagagat cggcgcccgc atcggtatta attccggggc ctgcatcgtc ggcaacatgg
    38701 gatcggagga gcgcatggag tacacggtga ccggggacag cgtgaacctg gcctcacgct
    38761 tggagggcgc cagcaaatat tacgacacct tgattttgat cggccagcga acggccgaat
    38821 tggccaagaa cgacgttgaa gtgcgagaga tcgatctgct acgagtgaag ggcaaaaaag
    38881 acccggtcgt cgtctttgag ttgctggccc gcaaggggca agtggaggaa aagaagcggc
    38941 gcgtcatcga cgtctatttg gaaggattgg cggcctataa agtgaggaac ttctcgatcg
    39001 cctgcgccaa attttctgaa gcggtgacct tggacccatc ggacggtccg tcgcgggtgt
    39061 atctcgaccg cgcgactaat tattgccaga cgcctccgtc agcggattgg gacggggtct
    39121 atgaaatgac atcaaagtga gcggcggtgt tgaaggggag gaagctgtga tatcaccacg
    39181 agtgcttcgc gttgtcctga tcgttctgtg gatcggtctc ctggtctgga gcggagaagg
    39241 ccgggccgaa accgtctacg ttcaagccaa gaccgcacaa ctgcgcgccg gcaaaacgtc
    39301 gttggatgct gtcgtcggga acgtcaagtt cggcgacgca ctggaggtgg tggggcgtga
    39361 cggcagttgg gtggaggtca agacatccgc tggcgcgaaa ggatggatct tcgccaacaa
    39421 aacctcgtcg tccaaaccgt ccggcaccaa tgataccctg gccaaactcg ggcagagtat
    39481 gcgggggagc gatgcgtcgg ccacgacggc gtcggcaggc gccagaggcc tggataaggc
    39541 ttccgaaggc tatgccaatc gcgccggcgt atccgcgcgc gatcgtgacg ccgtggaccg
    39601 gatgaccgcg tatcagattc ctgatcagga ggtcgaagaa tttttgcgag aaggaggcct
    39661 cggtgaatat gcgaagtaat catggcctga cagcgacgat catcgccctc tcactgatgg
    39721 tggccggttg cgcggaagtg cagcgcgcca gtgaagatat cgcgcggcaa tccggtaatc
    39781 cgcggctggc cggtgccatt cacggagtcg gcaatgtggt ggggagtttg ttcccgatcg
    39841 ggtacgagga agagtcgtcg atcggccagg cgatggcttt gcaggtcgtc gcacggtatg
    39901 gcggagtcgt ggatcagcca gagctcgtcc ggtatgtgaa tctggtggga cgggccgtcg
    39961 cgaacacgtc tgaccggccg gatattccgt atcgggtggc gattctggaa catgaatcca
    40021 tcaatgcgtt tgccgcgccg gccggctaca tctttgtgac gcgtggtctg cttaagcagg
    40081 tccgcaatga agcggaattg gctgcggtcc tgggccatga aatcgcccac gtcagccaaa
    40141 agcatattct cgatgtcatt caacgcagca aacgcctggc cggggtaacc gaagccggcc
    40201 tgtcctacgc gacgagcaat ccagcggcgt tcaagaccgt gatcgacggc gcggtcaaga
    40261 agttgctcga tgaaggcctg gatcaggaca aggaaacgga agccgatacg atcggcgaag
    40321 tgtttgcgac gcgggtcggc tacgatgcgg aagcctatgt gaacttgctg acgcgcctac
    40381 gagacctcaa gggtgacgac cgggctttgt ttaagaccca tcccaacttt tcagcgcgta
    40441 tcgaggccgt gcagaaaacg attcagagca aacatctcgt ggccaccggc gtcttattgc
    40501 cggagcggtt tgctcgcatg accaagcgcg tgtagcgcaa gattgtgaaa tgggcagcca
    40561 gcggcgttct cgtgtcgctc agggactgaa cgtaccgtga agatacgcct cgcctctnnn
    40621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc gttctcgtgt cgctcaggga
    40741 ctgaacgtac cgtgaagata cgcctcgcct cttcgctcgc tgcggccttg ctggacagcc
    40801 catttgaaca tcttgcgagg cggctgaaaa aagtcctcca gctttcttct cgcatcattc
    40861 agactctcaa cgtagccagc cagtacacct cgggtcttca cttgctacgg ccccgcttga
    40921 gagtctgttt gagccgcctc tcaggctgct tcgattggtc gtcagcgctc ttctcgcttc
    40981 ccggatgagc catccctagg tcgccgagcg aaaccgcagc gtacacgtgg tgccttttcc
    41041 ttggttagtg gtcacgatca aggtcccgcc gttgtcttcc acgagccgtt tgacgttggt
    41101 gaggccgagt ccggtgccgt aggtcttggt cgtgaaaaat ggttcgaaga ttctcccaag
    41161 atgatcggga gcgatgcccg atcctgtatc ggtcacgttg atttccaccg tgcctccttc
    41221 cgcgctgcga gtcgtcaggg tcagagtccc tcctccctcc gtcatcgctt cccgggcatt
    41281 aatcagcaca ttcaccaacg catctttgat ctgttcagga tcgatgcgaa tcatgggcag
    41341 atcgtgtgag aaccgctgtt cgacccggat atgctcatgg ccaccttgcg cctcccattg
    41401 cttcagcgca gcgccaatga tcccttccgc agcacagtcg gccaggtccg gcggtttctg
    41461 tctggcataa cgcaaaaagt cgttcagtcg ctgagacagc ttgtcgacct cgtcgatgat
    41521 atagcgggcc atttcatgcc ggattccttc ttcctgggga ccctccagca cgacttgcgc
    41581 ggaacttcga atcacaccga gaggattgcg taggtcgtga gccagagcgg caaggagttg
    41641 tccgacggag gcgagctttt cctgccggat caattcatgt tgttgcgccc tgatagtgcg
    41701 cagattttct tccagcacgg ctcgcatcgc gctgaacgtg gtggcaagat cttcgatctc
    41761 atcgccggtg cggtattctc ggggcaaggg cgtagcagcg agggggagag cgtgatcagt
    41821 ctgagtcgtc gccagcgtga ggcgcagccc ttcggcctcc cgctgaagcg ccgtgagtgg
    41881 tcgcacaata cggtagccaa ccacaaatcc aaacatggac aacgcgatca ccagcccgaa
    41941 gccgatcacg cctaccgaca ggaggagcga atagatcggc gcataggttt cttcgggagc
    42001 ctgccggaca aaggcatacc atcgattgcc ttccaagctg gaccgggtca acggatggct
    42061 gaagcggacc ggcgaggctc ccacgattgc gtcacggccg ccgtgagcat cgtcctcggc
    42121 caccaaccat aagggatgat ccagagccag ccggttcatc aggttcgcgg gaatcaggtg
    42181 ggcagtcggc ggcagcaccg gacagatgag cggtgtccct tgcgtatcga ggagcatcgc
    42241 atggcctgta ttgccgatat ggatgggaag aatcagctgg gtgaggagcg tgtgccgaat
    42301 cacgaggtct acggcaccgg tcaccgcgcc tcctgccttc tcgacaatgg gaaccgcgac
    42361 gtggaaggtc gcgtcattga tggcaggatc gatcgtgacg ttgctgacat aggccgtccc
    42421 ggccggggcc tgcatggcct cgcgccacca cgattgatcg gcctgctgcg gtggcaaggc
    42481 ccgatcggtg gaggcgatga cgaggcctcg gtgatcggcc acggtcacgc gcagataatg
    42541 ccgcgcttcg cgtgcccaat cttcgaggta ggcggcggtg acggggctgt tgacaggccg
    42601 atgtgtggaa tgaacgcggg cggcttctgt cttgttgctg tcggccttgg attgagcttg
    42661 tgcgtgctga ttcgccacgc tgatggcgag gcggccggcg cgatcgattt catcgtcgac
    42721 ggcgccggcc aatcgaatcg aggtcgagcg agcaatttcc tggaaccctt cgccgatcgc
    42781 ctctttgagg ctggccgtgc tggagcggta cgtggcccac agtgccgcca tgccgggcac
    42841 gatgccgacg atcaataacg cgacaaaaaa tttcctctgg agtccgccgg tccgtcgtgc
    42901 agcgcgcatc acccttgagg ctgcgacagg ttatgtttcg ccatgcgata ccggagcgtg
    42961 ttgcgcgtga ttttgagcag ccggctggct tcggacacat tcccgccgct cttggccagc
    43021 gcctcttgca gcatcgcctt ttccacctct tcgactgaca accctaacga caggagcgaa
    43081 ggagcgccgg ccggcgacgc agacgattgg ggtgcggcgg cggtcaagga ggcagggaga
    43141 tgttcgggct gaatcatgtc ctgcttgcag gtgatggtga gccattccac gacgttgtgc
    43201 agttcccgca cgttgccggg ccaagggtac tgggtcagaa gcgcgagcgc attctgagca
    43261 atgcctttga tccggctgcc acgctctcgc cgggctcggt cgagaaactg agtcaggatc
    43321 ggttcaatgt cacccgggcg ctcgcgcaag ggcggcatgc gaagttgata gacgttgagg
    43381 cgataataca agtccaggcg gaagcggccg gccttgatga gatcgggcaa ctcctgattg
    43441 gttgcggcaa tgactcgcag atcgaccgga atactccgca ccccgccgag cggctcgact
    43501 acatgctgct ccagcacccg cagcagtttg gcctgcgcgg tcagactgag ttcgccgatt
    43561 tcatccagga acaaggtgcc ccggtctgcc atttgaaagc ggcccggttt ggattttttg
    43621 gcatccgtga aggcgccttt ttcataaccg aataattcgg attcaagcag atgctcggga
    43681 atcccggcgc aattcagtgc caccatcggg ccttgggcac gcggactggc caaatgaatc
    43741 gctcgcgcga gaagatcttt gcccgtgccg ctctcgccgg tgatcaacac cgtggcatcg
    43801 gtctgggcca cctccttggc cagccgtttc acgagctcca tttctttgga gacgctgacg
    43861 atccggtcga atccgaactg ctcctgcagc cctgcgcgta aggtgcgatt gtcccgggca
    43921 agttgacgga cttgcagtgc ccgggcgatg acgattttca gctcctcgtt gtccaccggc
    43981 ttggagatgt aatcgtaggc gcccttttgc atcgcttcga cggccgactt caccgtaccg
    44041 tatccggtca caatcaggac cggcacatcg ggcgcctgtt tatgcgcggc ttccaggacg
    44101 tccatgcccg agacggttcc cagctgtaga tccgtgatga tcatgtcgat cgaatcatgc
    44161 tcctggagca tgtgaagggc ttcgtcgccg gaacgcgcca gcaggagttg atagccggct
    44221 gctttgagca ccagctcaaa gagccgccgc atgcgttctt catcgtcgac gatcaggagg
    44281 gtgggggcgt gcatggtgtc gcaaccgctg aggtgggtcc ccgactcggc gaggacgtga
    44341 gtcgtagcct accactatcg gcgaaagaga aacagagggg agcttaccgg gaatcacggc
    44401 tcgatgatga ggcgggcgtg ccatttccgg ctggcgcggc ttcattggcc agtgcccagg
    44461 agacgttgcg ttggagtccg gcatgtttgg cgcgtcgaac cggactctgt ttgaacgtcg
    44521 ccgcgaaggt tgcctcgttc atgtccatca agctgcgcaa cgacggtgag gtggtgagcg
    44581 gtgtgggctg gaagcccggc tcgctggtcg gttcggcctg atgattatac ggacagatat
    44641 cgagacaatc gtcgcagccg aaaatccgat tgccgatacc ccgtcgcagc tcctcagaaa
    44701 gcgtgggctc gctgcctcga agttcaatcg tgaggtacga aatgcatcgc ccggcatcca
    44761 cgacataggg ctcggtgatc gcacctgtcg gacaggcttg gatgcaaagc gtgcaactgc
    44821 cgcacaaatc ggttccggcc tcgtccgcat cgagctccag ggtcgtcaga atttctccca
    44881 acaggagcca tgaaccaaac tcaggggaga ccaggttgga gtgcttgccg atccatccca
    44941 ggccggcctg ctgagcccag gctttttcca tgaccggccc cgtatcgacg taggcgcgat
    45001 ggcgatcctc aggtgccaga gtggcgagcc gttcagacag ctgcgttaac cggtcaccca
    45061 tgatgttgtg atagtccagt ccccaggcat accgggcaat ccgcccgttg cccggtcgtt
    45121 catcggcccg atgatccgtg tagtaattca ttccgacgga aataatggac cggcatccgg
    45181 gcaggaccag tgccggatcg gcccgtcgtt ccgggtcgcg cgtcatccag ttcatggagg
    45241 catggaagcc tcgctgcagc cattcatgta accggtcgag gaggcggtgg ggagaaacgc
    45301 tgggattgtg gttcggtgcc ggctgtggtg gcaaccggct gatgccgacg acatcgaatc
    45361 ccagctcgcg cgcggcttgt gtaatcagcg ctgtgagtgg ctcgggcatt acggtttcgc
    45421 gcactcgaaa cgcaccatga attggccggt gcaatggctc gaatcgcagc gggtgcattg
    45481 accggtaatc ttgtttttcc agtccgtccc ttttttgtcg aaggcgcaga aggtgcgacc
    45541 ggcttttgag acggtcgtcc agggtttgtc tgtcccggga aatgttgcga tgacacagcc
    45601 ggctggaatt ggtacttggc actgatgggc cggatcaccc ttggccatgc cgaaactgca
    45661 ggagtgttcg gtggtggccg cagagtcagc aggtgcttgt gcctgcgcgt cgaacggcat
    45721 gacatgaagc gcaatctgca cactgagtag gacaggggaa atcaggcgga cgagccagga
    45781 catcgtagag cgctccagtg aaaagggcat ggtaccacag ccttgtttct gcggcgaacc
    45841 ctcatctccg gcatgccccg ctatttccgg ccctgatctt ccgattctgt ccggacattg
    45901 ttcaaaaacc accgtcggtg tagggagaag caggaacgcg agggcgtaac ttggtgcaag
    45961 aacaggatcg ggcaagtggc atgttagttg cgagaaccgg tagaccacta gtgcgtgtgc
    46021 ataggtgtgc gacgggacca cgagatcccg gcatgacggg caacaatcct gtgttcaagc
    46081 cgttttctga ttgcaccatg gctcaggaaa aaattttgat tgtcgatgat gaagtggtgg
    46141 tggctgagga cattcgtcgc cagctgcggt cgctggggta tgttgtcgtc ggggtggtgg
    46201 cctcaggaag cgaggccgtg gatatggctg gagagcatcg gccggacctg atcctcatgg
    46261 atatcaagct caaagggccg ctcgacggga tcgatgccgc tcgcatgatt caagcgcgct
    46321 acggcacgcc ggtcatctat ctgacggcct tctccgatga ggacacgctg aagcgtgcac
    46381 ggtatacatt gccattggcg tatctgatca agccttttgt gagcagtgat ctgcgcgctg
    46441 cgatcgaact ggcgctcttt cgtcatcgtg tgctgcgtat tgccgagcag cgcggccgct
    46501 ggcttgattc ggtgctgcag tcgatgagcg aggcggtcgt gacaatcgat gctcagggaa
    46561 aggtgacgtt gctcaaccag gcggcggagg ggctgaccgg ctggtcccaa ccggacgcgc
    46621 tcggaaagcc gattcaagat gtcatggtcg tggtggatgc ggagcggcgc acgccgctcg
    46681 tgaatcccgc cctgtcagtg ctgcagcaga tcaaggctgg ggatcggggc acgcgcccgt
    46741 tcctgctcct gagtcgtcaa ggcgtcgagc acgtgatcca cgagagcgcg accgtgatcc
    46801 acgatgaaca aggcggcctg accggagctg tgctggtatt ccgcctcgcc atcgagtagc
    46861 tattggggac cgcggctcgc tgccgtcagt cacggattta ccgatcgcag aaatcggtat
    46921 cggcggtcat tccgtcatgc tctgcactcg cactccaggc agtcaatcag tatacttatc
    46981 ctatgacctc ttccacggtg attggttcat cggcccgtcc gtttcctgga gtacccatga
    47041 ttcctcgtgc caccgtcgaa cgcgcctcga tccccttcgt aacacccagc gttctgcgac
    47101 tcgccaggcc cttcggtaga gactgatgcg tgccatggta cagctcatgt cttttctggg
    47161 aacgctgctg ggctgcttga tggcattcgt gttggcgacc acccctcaat cctgggcagg
    47221 agaggtcggg tcgatccatt tgcttcggcc ccttctggtg cagtctgaac cgggtggcgg
    47281 ggtcaaagcc accgccacgc tgctcttccc gggcagtccc tccgtcctgc attccattct
    47341 caccgaatat cgcaagtggc ccgagctatt tgaaacccgt atgcgcatgg cacaggtgga
    47401 ggagcgcgaa ggccatgtcc tgacggacgt ctatatttcc catgcgctgc tgccaggcga
    47461 gcagcgattg ctctgtgagt cgcaggtgtt acccggcggc gggttggtga ccgatctcaa
    47521 aggcggagat ttcaagcagt accatcgcga gtggaggctg caggcggcag gtgaaggcac
    47581 tcaaacgaga gcggagtttg aattgctcgt cgagcccaag accatgattc ccgattggtt
    47641 gatcgccgtt gccatggagc gggagctcac ggtgcacttt cgtctcctgc ggcaacgggc
    47701 gctggatctg gttacgaagg gaaggtaaag gtcgtcagtt cggccttctc gacgcctcgc
    47761 ttgacgaacg cgctgatctc cagccctcgt tcgatgcgga cgatatcctc caatttggtt
    47821 ctggtgcttg gatggagggg gacgaacagt ttgcagcaat cctgatcggg ttcaatggat
    47881 gtggtgaagc tgccgatctg ttgggcctgt tccgtgatct cgattttatc catgccgatg
    47941 agcgggcgga gcaaaggaat ctccgccgcg ttttcgacga ccaccagatt ctccgctgtc
    48001 tgcgaagcca cctgaccgag actgtctccg gtgaccaatg cccagcagcc ttcccgccgc
    48061 gccagctcct gtgcaatctg caccatgatc cgccggtata acaccacacg aaacgccgcc
    48121 ggcgcattgg cgacgatctc ctgttgaatt tcgccgaacg ggacgatgta gagcttggag
    48181 tgatattggt acgtcgttaa aagttggacc agctcctgga ctttttcctc cgatgcgcgg
    48241 ctgaccagcg gccggccgga aaaatggacg aagaccgcct tgcacccgcg cttgatcatg
    48301 cggtaagccg cgaccgggga atcgatacct cccgagatca aacaggcgac tttgccgctg
    48361 gtgcctaccg gcatgccccc gggaccttcg attttcctcg ctgcaacatg ggcttccttg
    48421 gtgaggagct cgatgtagag ggtcacgtcc ggctgtttca gcttgacggc tttccccatg
    48481 gcttcatgga gataggcgcc gacttcgcgc tccacatcca tcgacgtcaa cggcaggcgt
    48541 ttatccgcgc gtttggccgt tactcgaaac gaggtaaacg tctgtgtccc aagttgacgc
    48601 tgcgcaacct gcttgatgct cgacagttcc ggctccctga tgtctaaagg cacggattcg
    48661 ctcagcagga agtttgagac gccgcaaatg cgacggagcc gctccgtcac caggtccgga
    48721 gtgacgtcct cgggaatcgt cacccggatg cgtccctgca gcgattcgat ccgccggatc
    48781 ttcaggtctt tgagggccag gcggatgttg cggacgagcc gctgctcgaa gaaatcgcgg
    48841 ttacggcctt tgagggcgag ttcgtggtag tgaatgatcg cgcaatgcat agtcccggtc
    48901 gctgaaaact tcctccggcc gcgttctcag tcgcttgtcc ttcctcacgt acccactcgt
    48961 acgctcggtc ggccaagctc cctgcggcct tgccggagaa atttttgagc gacctccttt
    49021 taattaagtt cggtattggt ttgtgaagaa ttgccgatcg tcgactgctg gcaacttcgt
    49081 tctcggctcg acgatatcct caacgtacca ctgcgggtac gcctccggta tctgtctcgc
    49141 ctgcggcctc gttggacaac cattttgagc atcctgcggg aggtttagag aggttagaga
    49201 gaatgaagcg ctgattctag aaaccgttcg gcgtcgatgg cggccatgca gccggagccg
    49261 gctgccgtaa tggcctgccg gtatttcgca tcctggacat cgcctgcggc aaacacgccg
    49321 ggcacactgg tgttcgtccc gcgcgaggtg aggatgtagc cctgctcgtc catctccaat
    49381 tgacccttga cgagatcagt gttgggacgg tggccgatgg caacgaagac gccggcgcag
    49441 gggaggtctg tggaatggcc cgtcacgagg ttcttgagcc ggacaccggt gaccagatct
    49501 ttcccgagaa tgtcttccac gatggagttc caggcaaagg agatcttttc attgttcatg
    49561 gcccggtctt gcatgatctt ggaggcacgc agcttatcgc ggcggtgcac aagggtgacg
    49621 gtgcgcgcaa acttcgtcag gaaggtcgct tcttccaccg cgctgtcgcc gccgccgacc
    49681 acgaccaaat cctggccgcg aaaaaaatat ccgtcgcagg tggcgcaggt cgagacacca
    49741 tacccggtca gacggtgttc gttcggcaag ccgagtcgaa tgggggaggc gccggtcgcc
    49801 aagatgagag cgctcgcccc gatcgtttgt tctccgtcga tggtcaggcg aaacggtctg
    49861 gtcgacagat cgatcgcgga gacgtcgccg gtcaggaact ctgtcccgaa tcgttcggcc
    49921 tgaccacgca tctccttcat caactccggc cccataatcc ctttcgaaaa cccgggatag
    49981 ttttcgactt ccgtcgtggt tgtaagttgt ccgccggatt gccagccttc gatgagaaga
    50041 ggcgacaggt tggcccgcgc ggcgtagatg gccgcggtca gacctgcagg tccggagccg
    50101 atgatcacga gatgatgcat acgcggatgc taacacaccg atgctgcgag ggaaaagcca
    50161 gaggcacggc atttgggtaa cgattcgcac ggatcacgga tgaccgaagg ctgctgggtc
    50221 gcagatccgc taggcgagcc tggccaattc gcgatagagc gatttgacca gcgtaaacag
    50281 gcgcgggcgg gcggtggttc cgtcgaggac aaagtcggcg gccgccactt ttttcctcag
    50341 gggccattga ttacggatgc gtcggagggc ttcgctgcgg gtcaatccat tgcgtttctt
    50401 caatcgggcg atctgggttt cccgatcggc ggtgaccacg atgaccttgt ccacgcgcgc
    50461 atcaatgccg gcttcgtaga gaagaggaac gtcgtagatg acgacggcgt ttggatcctt
    50521 gcggacggcc agcctggtaa gtctcgcctg ttctcgcgcg acgcgggggt ggatgatctg
    50581 ttccaaacgg cgcagcctcg cgcggtttcg aaacaccatg gttcccagag ccgggcgatt
    50641 gatcgtccga tcgggattga tgacggcggg tccgaacgtc ttcacgatcg cgcgccaggc
    50701 cggcttaccc ggctccatca cctgatgtgc cagtgcatcc gcgtcgatga cgagagcccc
    50761 acatcgttcc aacatcttgg cgaccgtgct tttcccggtg gcgacgccgc ccgtgaggcc
    50821 cacaaaaatc atagggcgcg aagataccat gggggtccga tccccgcaac gaggctcggt
    50881 tgacttctcg ctatggccag cggtatgacc atcctatgaa aataatgaac tattggatat
    50941 tgcagatcgc atttctctgt gtctcggtcg gcacggtgag catggtctcc gtgaaagcgg
    51001 aagaacctcc agcagaacgg cagccgctag tcgtggcctt ggacggctcg ggccagtatc
    51061 gctccattca ggaagccgtg gatgccgcga aaaaaggtga caccatcctg attcgccccg
    51121 gcgcctaccc cgaagatgtg accattcaca gcaaagaaaa tgttcgcttg atcggggccg
    51181 gcatggatca ggtgacgatc cttggccgtg aacgggtggg cgtgtttcac atcggcaaat
    51241 ggccctatgg cgcgacgaat atcgagatct ccggcatcac cgtcaatgaa catggcgggc
    51301 atgcgatggg catgtttaat ggccgtggca tccactttca tcatgcgcgg gtgaaaggca
    51361 tgctgtttac ccagcaagtg gaggatgtgc ggatcgagaa ttgcgatatc ggcggcagcg
    51421 aaaccaccgg tgttcaattt gccaactcgc aggccgtcat gcgggggaat ttcatccacg
    51481 acaacgacca cggcgtcagt gtcgcgggaa aatccaccgt gcggctggaa cacaacgtca
    51541 ttacacgaag cctgttcgag gccgtgatcg tcaatgatca ggcgcaggcc gtcttggttg
    51601 ggaacacgtt cgtgaaaaac ggcggtggtg cggcattttt aggcacgtcg caaaacgaag
    51661 cgtcggggaa catcgtgagc ctcaatacct acggcttcgt cgtcgcggcc tcaagccgtg
    51721 tcctcttctc ctacaatgcg atgcagaatt caggttccaa ctatctccgg tccggtactc
    51781 cgaaccagcc ggcgcctgaa ctgaaacctg attctgattt gacggtggac ccccgtttcg
    51841 ttgacactgc gcaagatgat ttcaggcttc gagcggatac gtcgctcgtt aaaatcggca
    51901 cctttcccta tctcggcgcg ctcccgccct tgagcgcatc gcgctgaact tcagtcggaa
    51961 cgcgcaacaa attaagaggt tacctggctc gtcttagcga cggaggtcct gtgctatgct
    52021 tcatggtgag ccatggacgt atagggcgcc tgtctggctc aagaattgct gcactacgac
    52081 cgacaaagga ttggaggtaa gacgtggcca gctttcgctt cgatctcgaa aaacgcagta
    52141 cggacgggat tcccaacctg gcggaggtgg agtccggtaa gctcctgggg gccattttgc
    52201 cgatgtcgga gcaggcgcaa ctccagttac gggatagcat cgaggtcatc gactatctca
    52261 tgaaccagga aaccgtcgtc tggagctagt gtccctgcct cctcgcgcaa gcaatcctcc
    52321 cgcgctcctt cctgtcccgc atttcattgt atgtcccgct catgcagttc accgtcctac
    52381 tcacggaccg gtgctgctac acgttccatt cgtgatcgac cgttccattc actcggctct
    52441 ccactcctcc gaacatcagc agtgatcctc ctcaatcctt cacgaatccc tgttgaatct
    52501 ttttgatgcc cggtgcatgc tggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    52561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    52621 nnnngatgcc cggtgcatgc tggcgccgtt ttgggtcaag gacggccagc aagaggagtc
    52681 tgtgtcatat gtcgacatcg accgggaaga agtctgttcc tgtcccgcca tcagctgaac
    52741 agcagcgcct cgatgacgat gacctccgcc ggaaacattg gaagcggtgg ggcccttacc
    52801 tcagcgaacg ggcctgggga acggtccgcg aagactatag tccctatgga acggcctggg
    52861 agttcctgcc ccatgaccat gcccgctcga aggcctatcg atggaacgaa gaaggattgg
    52921 ccggcatttc tgatcgccac caatttatct gtctcgcgct ggccctctgg aacgggcgcg
    52981 acccgatcct gaaagaacgc ctgttcggac tgacggggaa tgaaggcaac cacggagaag
    53041 attgcaagga gtactacttc tatctcgatt ccacgcccac gcattcctac atgaagtttc
    53101 tctacaagta cccgcaggct gccttcccgt acacgcgact catcgaagaa aatcgccgcc
    53161 gcaaccggac agacccggaa ttcgagctgc tcgacaccgg tctgtttgac gacgaccggt
    53221 acttcgatat cgttgtggag tatgccaagg cgtcgccgga ggatctgtta ctgcgcatca
    53281 ccgccattaa tcgtggcccg gagccatcgg agctaacggt gttaccgacg atctggttcc
    53341 ggaatacctg gtcctggggc attgatgtgc gccgcccgcg aatgcgccag ggcgaatcga
    53401 gccccggggt gagtgccgtc gaatttgatc atgagtatta cgggcggcgt cgattcctgt
    53461 gcgacggagc cccggatttg ctgttcacgg aaaatgagac caatacacgc cgtttgtatg
    53521 gcgattccga cggggcgcgg tatgtgaaag acgggatcaa tgactacatc gtccaaggag
    53581 acaagaacgc cattaatccg gaccggatcg gatccaaggc cgccgcgcat tatgtgcgtt
    53641 cggcaatgcc gggacagcca gtcacgattc gcctgcgctt taccaacggg tctgcggagg
    53701 gagcgctcga tcctgaggca ttcgacgccg tctttgcagc gcggttgaaa gaagccgacg
    53761 agttttacgc acatctggcg cctcccgatc tgtccgccga tgcgcgcctg gtgcagcgcc
    53821 aggcctttgc cgggctgctc tggagtaaac agttttatca ttatgaagtc gatcgatggc
    53881 tcaaggggga tccggccggt ccggagcctc cgccggagcg gttgcgcggc cgaaacgccg
    53941 actggaccca tctctacaac gccgacgtca tctccatgcc ggataaatgg gaatatccct
    54001 ggtatgccgc ctgggatctt gcctttcact gcctgccgat tgcgttggtc gattcgcgat
    54061 ttgccaaaga ccagctcgtg ctgatgcttc gtgaatggta catgcatccg aacgggcaga
    54121 ttcccgccta cgaatgggcc ttgggcgatg tgaatccgcc tgtgcatgcc tgggcggcct
    54181 ggcgcgtgta caaaatcgag aaaaaacgta annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    54241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    54301 nnnnnnnnnn ngcgagtaag ttcttcgagc atttcgtcta catctgccgg gcgatgaaca
    54361 acatcggcaa cgccaagatc gagctctggg acagggaaga cgggtttttt tacgacgtgc
    54421 tgcatctgcc ggacggcaag accttcccca tgaaggtccg gtccatggtg ggactcatcc
    54481 ctctatttgc cgtggagaca ttggattccg aactcatcga ccagttgccg cgcttcaagc
    54541 atcgcatgca atggttcatc gagaaccggc ctgatttcgg cttgcacatt gaaacccaat
    54601 cgcaagacgg cgaagtgcgc cgctttctct cgctcgtgaa tcggcgacgg ttggccaagg
    54661 tgctccggta tttgctcgac gaacaggaat ttttgtcgcc tcatggcgtg cgtgccctat
    54721 cccgttatca caaagaccat ccgtacgtgt tttcgatgat gggcaccagc cactgcgtcg
    54781 aatatcaacc ggcggaatcc tccaacggcc tgttcggcgg gaactcgaac tggcgcggtc
    54841 cggtctggtt tcccgtgaat tatctgctga tcgaatcgct gcagaagttt cattattatc
    54901 tgggcgaatc atttcgcgtt gagtacccga ctgggtcggg gcagaggcgc aatctggcgg
    54961 aggtggctgc cgagttgtcc cgccgcctca cgcatacatt tttacgggga ccggatggtc
    55021 ggcggccggt gtatggcggg acggcgaagt ttcagcagga tccgcactgg cgggatctcc
    55081 tgttgttcta tgaatacttc catggcgaca acggcgccgg cctcggcgcg agtcaccaga
    55141 caggttggac cggtctggtg gccaaattga ttcaacagtc aggcgagtag gccggagatc
    55201 cgtcatgacg atcgattcga gacattgcca ggacctggac ggggcgttga atctggagtg
    55261 gctggagacg aatggccgcg gaggatatgc gtccggcacg gtcgctggtg ccaatacgcg
    55321 gcgctaccat gccttgctct tgatcgccag acatccgccg gtcgatcgcg tcgtgctggt
    55381 caatcacgtt gaggaattcg tcgaaagcgg cgggcggaca ttcccgttgt cgaccaatct
    55441 ctatcacgac gccgtgcatc cggagggctt ccagtattgc gaagagtttt ccgccgatcc
    55501 ctggccgacg tggcgttatg ccgggcacgg tattcgtctg atacgggaac tgttgtgtcc
    55561 gcaggggcgc gatctcgtga tcctgcggtg gcggctgctg ga
//
LOCUS       DAOQDS010000022        55571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_180_length_55571_cov_20.492291, whole genome shotgun sequence.
ACCESSION   DAOQDS010000022 DAOQDS010000000
VERSION     DAOQDS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 55571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55571
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180_length_55571_cov_20.492291"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PK589_07215"
     CDS             complement(<1..225)
                     /locus_tag="PK589_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19428.1"
                     /translation="MILSDYTPVTEKAGDWVTPEALSMVYTRYRFARDFCRGRRVLEV
                     ACGPGVGLGYFGRYADQIIGGDLTSRLLRQA"
     gene            complement(222..1334)
                     /locus_tag="PK589_07220"
     CDS             complement(222..1334)
                     /locus_tag="PK589_07220"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNJ19429.1"
                     /translation="MAYHVPFIDPRSHYAKLKPEIDRAIIDCLANGDLVNRHQLKDFE
                     QHLAEFVGVKYAVGVNSGYHALLFALLGAGVGAGHEVITVAHTFVATVSAIVHCGAQP
                     VLIDVGPDFNMDVDLIERAITPKTKALLPVHLNGRVCDMERILALADKHGLAVIEDAA
                     QALGATFDGKCAGSQGRAGCFSFYPFKVLGGFGDGGAITTNDASLARMATLLRYNGED
                     RATGEYHYHGQTALLDNVQAAVLDVKLRHLPEWIAHRRQIAAAYHRGLAGISQLRIPQ
                     FDESRRQDIFQNYVIRTTVREPLRAYLKEQGVETLVHWQKPMWEHKGLGLLVPDVPET
                     LAICREVVSLPMSAETTEAQVEQIVAALRAFFSTQP"
     gene            complement(1337..2326)
                     /locus_tag="PK589_07225"
     CDS             complement(1337..2326)
                     /locus_tag="PK589_07225"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ19430.1"
                     /translation="MRILTYAQAIREAHAQLLAEDPRVFLIGQGVRNPWYAGTSLQDL
                     DKEFGTHRVIDSPVSENATTGAAIGAAITGMRPIVFHPRMDFMLLAMDPIMNQAANWS
                     YLFAGRVSVPIVIRAVINRGGEQGAQHAQALQALFAHVPGLKVVMPATPYDAKGLLIA
                     AVRDGNPVLYIDDRWLYDLQGDVPEGLYEVPIGSAALRRSGRDVTVLATSYMSKEATK
                     AVETLTQRGIDVELIDVRSIKPWDRNLVYSSVGKTGRLVIADAAWMSGGIAAEIAASV
                     AGEVFHALKAPIGRVCLPDAPAPTSAALEEAYYIGAEEIVSTVEKVLVSSTRS"
     gene            complement(2331..3332)
                     /locus_tag="PK589_07230"
     CDS             complement(2331..3332)
                     /locus_tag="PK589_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNJ19431.1"
                     /translation="MNFPDVHTGALSRDQLISLLRAMQRIRMAEERIGELVETREVRT
                     PCHLSIGQEAIPAGVCAALQQDDTLWGGHRSHGHYLAKGGDLRGMMAEIFGKATGCAR
                     GRGGSMHLVDPAQGIFGTVPLVAATIPLAVGAALSAKLRGTQQVAVAFFGDGATDEGH
                     FHESLNLAALYRLPVLFVCENNLYSTHLTLKERRVKDNIVESAALHGLPGVVVDGNDA
                     TAVYQTAHQAVQRARIGEGPTLLECRTYRWRGHVGPAADLEVGTDRRRELDDWQRRDP
                     IAQCRGLLKVHGLSSEVFDRLEAEIRREIDEAVSFARLSPAPDESELLQHVYARGGV"
     gene            complement(3329..3958)
                     /locus_tag="PK589_07235"
     CDS             complement(3329..3958)
                     /locus_tag="PK589_07235"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNJ19432.1"
                     /translation="MKRVFDVLCAVLGLMLLSPMLVVIALLITLGDGGSPFYRGLRVG
                     RNGRPFRILKFRTMRSDAERSGVTSTAKDDPRVTSVGAVLRRYKLDELPQLWNVLCGE
                     MSLVGPRPEVQRFVDLYTDEEKAILAVRPGLTDWASLWNIDEGAILEGSADPDRTYCE
                     LIRPKKIRLQLAYVRQAGFRTDLDILLRTVGAVLFRVKFREPRVLELGA"
     gene            complement(4321..5499)
                     /locus_tag="PK589_07240"
     CDS             complement(4321..5499)
                     /locus_tag="PK589_07240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ19433.1"
                     /translation="MSFTNSQAETPWVSGPFFHTYPIVSIIIPCRNERAFIAGCLESV
                     LANDFPKDRLEILVVDGLSDDGTREILDGFSRDHAFLSVLDNPKRVTPAALNLGIKHT
                     RGPIVMRMDAHARYDSHYISRCVDALGKYKVDNVGGIWKTLPRTETLAGWGVVKALSH
                     PFGVGNSHFRLPQGSEPKFVDTVPFFCVRRWVFQELGGFNESLVRGEDMEFSLRLNQP
                     GVFNERLTRGQDMEFSLRLRKAGGRTLLIPDIVSYYYARSDIKSFWCHNWINGVWAIL
                     PFAYSNVIPVSLRHLVPLFFVGSLVSTLVLTAWQGTFGWMFAGILGAYLLANVVASLH
                     VAWEARQPQYAVLMPIIFAMLHVGYGLGSLWGVVRLLTIREFWQKIFNVETNRAAPAR
                     "
     gene            complement(5527..6561)
                     /locus_tag="PK589_07245"
     CDS             complement(5527..6561)
                     /locus_tag="PK589_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNJ19434.1"
                     /translation="MFRVYYWTKDGIPAREWHRFFLGNFDGTMLDETLGWKATENYRA
                     NRAEKTKQGKSYVVTLSQNRYGFRQFGEIGSDKFKILILGDSFTHAREVSDSKTYYAL
                     LGRERPYEVFAYGVENYGTLQEYMILDRFFDLIKPDLLVWQYCYNDFANNDPLMERRT
                     MLHIDFRQRPYWSKGEIIRDMPSSILGKFRGLAYKYSRFIVFLLSRMDRLYAAYLLDS
                     VEAEILRSGAQHPDFRRAVRTTDELMATVRARVGATPVVAFNCKAVEPYNAALKNISG
                     RHDIIFLDEIAEAVDFAARQGRDVFHADGAHWSEEGHKLVGEMLVKRLEEFRVNRSLY
                     RYREAAAPPN"
     gene            complement(6651..7955)
                     /locus_tag="PK589_07250"
     CDS             complement(6651..7955)
                     /locus_tag="PK589_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HNJ19435.1"
                     /translation="MFLFVQGVLILACIALIARIAEGSWLSPGALFALLWSWYSLTAL
                     LFRVPSETLILGIVWIAGSGVLMHLGCLWGTGSQRVENQCEDKRFIYPYLRAVTGTLV
                     FIGLVRISYIILDGSPSLGEALSVENLSQIVMQNRATFVYGSEKLTFIERVLFVAFYA
                     APLFGGILFQVAVSNMDKAIAVLAVIAPAAHNLLYGSRMGAMFGGAFWISAYMAARLL
                     GGRSGKAHSDVSNLRALICIAVAIVGTSLVVLSLRYGDEEVSTLSLLLLAASDIFSFL
                     PSFAILLDKGTFSFSDLTYGDRTFGRIFEFARLGTEDHYIYEAVDVGYTSSNVYTVFQ
                     GLIDDFGHIGALVSALIFGYIAGHSYHQVLRGKVVAVPFLTAVYAAVMISFSFSLFTY
                     TSTTLALISFGLYIVLMAKEVRPDSSLDSSRLRLSRHQSQSQ"
     gene            complement(7965..9008)
                     /locus_tag="PK589_07255"
     CDS             complement(7965..9008)
                     /locus_tag="PK589_07255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNJ19436.1"
                     /translation="MKKILVVQTWGIGDMVMTTPMVHALRLAQPSAHICVVAGSDSAA
                     EVIRGSRLCEEVQVMSFRRSSMFTILRFFVRLRMQQFDVAFIASGLSPKIALGLKFLS
                     GVRTVVGDGGGAKGWGYTHWRRVDNTNHRVMENAEILKLALPNCGIGDLHFEFDEQAR
                     TEAARIWTNHHLDTCDVLGVHPGGQGSECPDKQIPQELCREVVQEYLARDETRRVVLF
                     FGPDETGLIKSYDWGLERVIILSGLRLTVVAATIGKCQAVLSGDTGLGHIAAAVGTPV
                     VTVAGPTDIVNTRPWGNKHRVVQTEKRPDCMPCYQTVLYGKCPHGQECMRSIDAAEVL
                     KVLMCLDDRQNVR"
     gene            complement(9015..10541)
                     /locus_tag="PK589_07260"
     CDS             complement(9015..10541)
                     /locus_tag="PK589_07260"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNJ19437.1"
                     /translation="MSMGHESRTALSVQTWTSASALLFGVTGISKVLGFVRELLLASY
                     FGAGREIDAYAVAYAIPLFLAGGIGYAFSTAIIPGYHSMVAEHGKQAGLHYLSTSCVS
                     SVAWSFILLSPVWGAPEYLVKFAAPSMSAATIRLTVELMGWLSIYVLIQNCVYVLSAV
                     FHAFNHFRLPAVSDLLFNVVVIGVLMALASNVGVHALVIGNLGGIFICLLVLMGIILR
                     THGAHFGALSFTGLVHPILACLPVALYYVASQFPGLLANYFASGLGEGSIAAFSYART
                     IVAGLISLITVSVARAAYPTFASLFTYGREEQFREFVAGLARLILYGFLPLSVLCWVY
                     QKPLLRLLYQRGAFDEAALTLTATAFGFLAIGLTFGAWEPVGTRALYAAGDARSPLLA
                     TILSACLVVPLCLMLTPALGLAGVALSLALAFAVDSAMQVISLGWRLNSPIWKDLAWF
                     SMKCATCALFATTSFLVMPVDHGWQATGGGVLYICSYGLLTWWLVRPVRSMWNVAIVR
                     "
     gene            10658..12931
                     /locus_tag="PK589_07265"
     CDS             10658..12931
                     /locus_tag="PK589_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19438.1"
                     /translation="MKPLLSHRFPISPLWSGLLLALLYLNVHDVLRAEESASPTTSHV
                     VDMRFSTTTFLLNLEKRGLEDNTFTNGLGRDTTLIGNLINATLYKRLLPSLEAEIGVF
                     ANMPFGHDHEISQVRPVVRLTYQPVEQITAVAGTIRTPHRDFHDAVFDNGNRFLRPIE
                     QGAQMLVDSTHYQQDLFINWEQAFRGSAPNRYDVGYAGQLKFGPLHFNGQAHWVRNGQ
                     ALFKLDRSFSTRDNLVVATGPELVLEPSRWFASTAWWHQIGISFTYLTSYNQADNGSD
                     ASRGRGYELRTWLDLAGWRPYLAFWKGRNFLSQQGDPEFRASNFPEFGLSKTIRLGKR
                     ASIEFGAQARRIRAFFTEDGLNYMNRDTFKWVNQEYLVFNWDWDTNHADLIGDVWASL
                     SRPFDDRQDAPSERRFTAKLDTLTYVYSISYHGVRQIGGIPVSNHTFAGQYLSPVLQY
                     RVSPQLTLDAGLFAGLPVGDTQSFHTVQPILSAQYEMVPDVRLVAGTLHRNHPFVDAL
                     FNDATLFSRPVEQGFQLLVDREHYQQDLFINWNQIETYQKAERFDVGYAGRVAAGPFA
                     LNGQVYWTHFGGAQYSEARTFFGPGLPRDRPAGNNFLTAFGPQFTWQPKQYWPQLSWF
                     REIDVMALYLTSQNEPTQSSSPIVRGRGYQLTAGVNLDGWRPYITLWRGEHFVTERGD
                     PAYYAGDFTEFGLFKDFILPAGFSLRLGGFGRTIERHLTHTEYALLNWSWDQTPWRGF
                     CQRPTLLHRSESSCGQS"
     gene            13023..13646
                     /locus_tag="PK589_07270"
     CDS             13023..13646
                     /locus_tag="PK589_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ19439.1"
                     /translation="MRRSSIGLLFLALLCIPAVTRAENYVAAGIGVNAPSFDTTDAGK
                     VSLQKDLFYGGKIGHYFNDRGYNWFGLEVDMYRSTPGIKQQTLPTSSNPRLSGPMPGA
                     DLQVHSLAFNALVRVTGYQYKVEPYAGLGIGLNVGNISNGNFRPEASFAPSFNVLAGI
                     RYYMTESIAPFIEYKYNFAQFKFDRSNVTADYRANLFMFGIAYHFGR"
     gene            complement(13735..15435)
                     /locus_tag="PK589_07275"
     CDS             complement(13735..15435)
                     /locus_tag="PK589_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNJ19440.1"
                     /translation="MTPDPIFFRDLAYVFIAAVVGGTSAYLLRQPLILGYVFGGILIS
                     PLTPGPSISDVHSFELFAEIGVILLMFSIGIEFSLNDLLRVRWVALLGGPLGIFLSIL
                     LSVGLGHLVGWTMIQSVVIGSVVSVASTMVLARLLLDSGDLRAKHGRVMIGITLVEDL
                     AVVVLTVLLPALGTLEPGRFATIGKALWLAVLILVPFSYLAAKAIPPLLTRIAKTQNA
                     ELFLLVSLSLGIGTAAVTQMAGLSLALGAFLAGLIISGSEYGHETLARLLSLRDAFVA
                     LFFVTIGILIDPRIVWSNLPLLGLMLGLILIGKFVIWTLVVRLFRYAWGTALLVGVGL
                     TQIGEFSFVLVQVAKTAGHVGEDVYNATLAASLLSILLNAPFVRYAPRWVAALHLRMG
                     RAAMPMQGGVQDEQAGHVVVCGFGRMGSTVGLALDHFGIAYTVIERDPDLVRQLRRRG
                     ISCVYGDASQTELLTAAGAERAALVIVALPVIHEASLTVRRLRGLNEKIPLLVRAHGF
                     REAEDLRAVGATEIILPEIEGAHTLIRHAFQALKVPKPDILAYIKSGQHFRASDADGE
                     "
     gene            complement(15894..15969)
                     /locus_tag="PK589_07280"
     tRNA            complement(15894..15969)
                     /locus_tag="PK589_07280"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15934..15936),aa:Cys,seq:gca)
     gene            complement(16071..17270)
                     /gene="ispG"
                     /locus_tag="PK589_07285"
                     /gene_synonym="gcpE"
     CDS             complement(16071..17270)
                     /gene="ispG"
                     /locus_tag="PK589_07285"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNJ19441.1"
                     /translation="MHITRKKTRQIQAGSVKIGGDAPISVQSMCSTDTRDVKATVEQI
                     RQLETAGCELIRVAVPDDEAANALPKIKAAMSVPLIADIHFDHRLALKAAEVVDCVRI
                     NPGNIGAWWKVQDVIKAVNERGIPLRVGVNGGSLERPLLDKYGWPSPEALSESALNAV
                     HALEDEGFTNMKVSLKASDVHHAIDAYYLFSTQSNYPLHIGITEAGTAMTGAVKSAIG
                     LGWLLSQGIGDTLRVSLAADPVEEVKVGFEILKSLELRHRGINVIACPTCGRVEIDVV
                     RMANELEKKLGHIKTPLNVSVLGCVVNGIGEGKEADIGIAGGEGKGILFKKGKLMRKV
                     PMDELMDTLIHEVEQLAKEKEAEASGVTHNGSSDGQTEEGWELMGHAPDEQSTIVRDI
                     PVLPSKP"
     gene            complement(17285..19228)
                     /gene="dxs"
                     /locus_tag="PK589_07290"
     CDS             complement(17285..19228)
                     /gene="dxs"
                     /locus_tag="PK589_07290"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNJ19442.1"
                     /translation="MSLLKNIHSPADLKRLSPEQFPELCQEIREQILAVVSNVGGHLA
                     SNLGVVELTVALQYLLDTPKDKIVWDTSNQAYTHKLLTGRREQFHTLRQYGGLSGFCK
                     REESAYDTFNAGHAGTGVSAAFGMVEAREQRGEKHKVVCVVGDGAMTAGMTLEGLHHA
                     GGTNKDFIVVLNDNQMSISRNVGAISAYLNRTFTGEFYARMREETGQLLRKIPHIGAE
                     MQKIARRAEELAKGAILPGLLFEELGFQYAGPIDGHNFEHLLPTLENVLKMKGPVLLH
                     VITKKGLGYQAAMDNPVWFHACPPFVRETGVPAKKAVRPSYTSMAVDALIKVARQDKR
                     VVAITAAMCEGTGLNAFEKEFPERIYDVGIAEQHAVTFAAGMAAQGMKPVVALYSTFL
                     QRAYDQVVHDVATQNLPVTFCIDRGGLVAEDGTTHHGAFDFAFLRHVPNMVVAAPKDE
                     NELQHMIKTCVSFDGPASVRYARGVSLGVPMDPEPTTLPIGKGELLREGTDVAIVAIG
                     VTVWPAMKAAERLAQEGISAAVVNARFAKPLDTELILKTAKNVRCLVTVEEGCKMGGF
                     GSAVLETLSEAGVMLRTKILGLPDWYIEQGPQDLLRERYGLTADGIYNSVKQLFGAGV
                     VADDAARLASLVGSLPHGDEQGS"
     gene            complement(19409..20428)
                     /locus_tag="PK589_07295"
     CDS             complement(19409..20428)
                     /locus_tag="PK589_07295"
                     /inference="COORDINATES: protein motif:HMM:NF022825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HNJ19443.1"
                     /translation="MNGRVLLMRHLVYFFCLIGLSCGLPLLAGAEGFGPFPVRNFQAL
                     DQLVLAMPGERAAVLRKGDLDIRLEAANTATIARDREEQADVTMKFETVRAGLFLRYG
                     LTERLEVGAEIPAYHRYSGFMEGAILGVERGTTGVSPPRKALRDTGYAFNVSNGGRTM
                     FQGSENATGLGDVSFYGKYQLLQETGSQPALSIRMAVKAPTGDTGQVFGSGHPDAGIG
                     LALDKTFATGWILYANVNGIFPTGRIAGLDVQPVVSGLVAVEYLWTENFSITAQFDYY
                     SPPFHGTGTRVLDKGVTESVLGVSYRILPRLLWQVYGVENLDFITGSAADFTLSTVLT
                     YRFRS"
     gene            complement(20437..21642)
                     /locus_tag="PK589_07300"
     CDS             complement(20437..21642)
                     /locus_tag="PK589_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNJ19444.1"
                     /translation="MQRHYFSSIRILLALAGLMFLSSPAPAGADTQIFPVPSVSTSKN
                     DGNDAGLIAPILISDPDGELKYLMAPMLIQNSIVGSRAVFNLFKYDPGGRQMKLIASL
                     TEKIERKVLFDYVDPAFGNGQYFLNFGGTFFKNATSRFFGLGQSTVQADESNYTAREA
                     RAYWRLGLYANEVTQVSVGQRVRQVQLQRGATDLPFSVEQFPTVDGIQGESIIVGHRA
                     SFYYDTRDSLVTPTDGMSVMAYAELNQNVKNGDHPVYSRYEIEVKKLFPSESKRAILV
                     VRADLQATIGSQVPFFEQSSLGGQNNLRGFGMDRYIDKHLIAFSIEERIHILRTKLAG
                     VTADFELAPFLDTGQVFNSFKDVSFQDYRMTPGVGFRAIVRPNVVGRLDYGYSREGGA
                     IFAGLDFPY"
     gene            complement(21676..22290)
                     /locus_tag="PK589_07305"
     CDS             complement(21676..22290)
                     /locus_tag="PK589_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ19445.1"
                     /translation="MHVRRWMVVGVLLALQLFVGGLSFALAGPATESIRGTVDEVLRI
                     LNDKELKAPAKHEDRRQRLEKVVAARFDYSEMSRRSLGAQWNQLSDKEKQEFVDLFRT
                     LLTNTYADRVETYSGEGVQYLNERTEKEYAEVRTKVLSGKTEIPMDYRLLNKSNDWHV
                     YDVVVDGVSLVNNYRGQFTKILHTSSYSDLVDQLRKKSEKIKAP"
     gene            complement(22377..22820)
                     /gene="mlaD"
                     /locus_tag="PK589_07310"
     CDS             complement(22377..22820)
                     /gene="mlaD"
                     /locus_tag="PK589_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNJ19446.1"
                     /translation="MERAKLELMVGIFVLVGIACLGYLSIKLGKLEVIGGHNYPIEAE
                     FTSASGLKPGASVEIAGVEVGRVRTIGLNSDRALVALAIQDGVKLYSDTIASIKTRGI
                     IGDKYLALSVGGGGDPLKPGDKIRDTESGLDLEELVSQYVHGKVN"
     gene            complement(22832..23593)
                     /locus_tag="PK589_07315"
     CDS             complement(22832..23593)
                     /locus_tag="PK589_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ19447.1"
                     /translation="MIKLVGVEKTLGKQAVLRGVDLTIPTGKLTTIIGRSGEGKSVLL
                     KHIIGLMQPDRGEVWIDGTDIARLKGRALNEVRKKFAMLFQGAALFDSMTVFENVAFP
                     LREKLRLKGDVVTRRVEEKLEQVGLKGMGHKFPAELSGGMRKRAGLARALVMEPEIIL
                     FDEPTTGLDPLMAKAIHDLIVAMQQQFRFTAVMVSHEIPEIFGISDYVAMLKNGRIAE
                     MAPSSEFVKTTDEEIREFIFVGGTVAPKGLPAESR"
     gene            complement(23678..24445)
                     /locus_tag="PK589_07320"
     CDS             complement(23678..24445)
                     /locus_tag="PK589_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ19448.1"
                     /translation="MEGIARIGRFTIDLTEQMGRMMLFVLSSFAWLTRPPFRFYQIVK
                     QLNFIGYKSTFVVVLTAVFTGMVLALQGYYTLRKFGSEAVLGSAVALSIIRELGPVLA
                     ALMVTARAGSAMTAEIGIMRITEQIDALDTMAINPLQYLIGPKLVASLIAVPLLVALF
                     DVVGIYGGYVVGVQLLNGNEGAYWSSIESAVEWKDVYGGILKSISFGLLISWVCCYKG
                     FHTKHSAEGLGTATTEAVVLSAVLILVWDYFLTSVLL"
     gene            complement(24500..25255)
                     /locus_tag="PK589_07325"
     CDS             complement(24500..25255)
                     /locus_tag="PK589_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19449.1"
                     /translation="MTTIGVFVATRWELAAVRHAFPASEVRAVGGIRCVVVRQPFVEW
                     CIIPMGVGPERAMATAKRILAEQALSAIWSTGFACALGPAGIGDVLIGTDVILENGGK
                     AGPSVPCAPAMVEWVRQAVHVQGLPMRSGGFVTVPRVLCLAEEKRAIAGRAGGIGLDM
                     ESAALGRVASEHRIPFAIVRTVSDLEDESLPLDFNLFLRPSGWAKGVVACLAHPTSLI
                     GLNRLRVQSQIAGAQLTALFRACADRALGEGIG"
     gene            complement(25310..27481)
                     /gene="shc"
                     /locus_tag="PK589_07330"
     CDS             complement(25310..27481)
                     /gene="shc"
                     /locus_tag="PK589_07330"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HNJ19450.1"
                     /translation="MRLLKNLFSRLSDSLLVSVPSRIRAARDFASAPVLRLVSSKPDA
                     PASDGAKRAPSHTTGQLEALEEAIRKSQAWFLARQDTTEGFWVAELEADTTLSSEYLM
                     LRRFMNCVDPERERKVIRYLKSAQLPCGGWPIYHGGPADISASVKAYFALKLSGVSAD
                     EPFMAQARTCILDKGGVVAANVFTKIALALFGQYDWRGVPSMPPEIMLLPKRFYFSIY
                     AISYWSRAVLIPLLIIFAKRPQCYVPPEQGIDELYTQPPAEIDYSTVPPFKKDRTWFT
                     ARNFFINLDALLKVYDRSPVEWVRQKALKGAENWMLDHMKGSGGLGAIYPAMANSVMA
                     LNCLGYKNDDPLVVKAMREIEELEIHDTVMDNGQCVDALHLQPCHSPIWDTALLINAL
                     SEAGMSQDHPALQKAATWLLSKQTKTVGDWIISSPGAEPGGWYFQFENELFPDVDDSA
                     VVLMALAKVRLPDEEQQRLAIRRGCRWVTAMQGSDGGWGAYDVDNNRIVFNYIPFADH
                     RALLDPSTADLAGRCLEMLATLGYDWTHPSVASALTFVKNDQEQDGSWYGRWGVNYIY
                     GTWSVLSGLRAIGEDLSSPYIRRAVSWVESKQNPDGGWGESCLSYADVAESGRGDSTP
                     SQTAWALLALMAGGVTDSFSLARGIHYLIRNQRKDGSWEEVRHTGTGFPRVFYLRYHW
                     YCQYFPLWALAMYRNLKTRGTTRADELRQLAYQSGQFRSPR"
     gene            complement(27705..31388)
                     /gene="smc"
                     /locus_tag="PK589_07335"
     CDS             complement(27705..31388)
                     /gene="smc"
                     /locus_tag="PK589_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNJ19451.1"
                     /translation="MYLKSLEMLGFKSFAEAKIQFPKGITAIVGPNGSGKSNVVDSIL
                     WVLGEQSTKTLRSEKMEDVIFNGTEVRKPLGLVEVSLVIGGLGELRLDAISGLPSQLS
                     EYQELMITRRLYRNGDSEYLINKTPCRLKDIRNVLIETRAGSKGHTVIEQGRIEQILQ
                     ASPQDRRELIEETAGIVRYKKQKAEALRKLESTQQNLLRVRDIVAEVKKQLNSLERQA
                     RQARTYQTLQQEAKGLEIELLSRDYRTMHADLESVDREAREVEALEAEQVAEQARLDS
                     EQEAIRLRMNDTAEAISRVRDTLAGTEQRQSQALTAAEVERNRTELFERQRVQASQEM
                     ERLAGDQEQAQVEIEDLRATLLQLEGDIVEQEAQFQQADAEAKALAGHRAAAVSEEER
                     ARKDVLNLAVLVANTEQSLAQVTARQQETSARAERVSREQEQMVAQVAGLDEQRELLA
                     AQREEASQRIQGLMAERQAAIERIEGLGGQITGLDRDIVRFSEDVAAVESRLGALQGV
                     VREEMGYGREGEEEGTALKTCDGVREALAEWLVIPSGLDRAVETILGERVRGWLVDQP
                     SAASRAVEFLKGKELGRGAFIPQQIRWATDQAAADSSASWWPALNGQPGVVGRAIDVI
                     RAEGASASTLSYLFDGIVIVESLAVALQLWEQHQWPAPAGPTYVTLSGETLDAAGVMT
                     GGGTSAGAGLLQRRREVLELEARRMEAVQALELARVAREEAASALGLGREEEQQLGRA
                     IREAEMLELSLQKDDTNVERQRGELHRRMDMLAEELQRGLAEQARLQEQLESSQAQLE
                     QWVAEKLGQENGLLQIRERLVVIESQGLAIQQRLTEVRLSLESMRGRREHAHSDIARF
                     TQRLDAAQRRAAELEEQVTGLVQATESSREEQTRQEALCRELGAEVDGIKSELIAAQE
                     RQAEEMSGLHTVEETLSTVRQSLSALHDRRMTAEVRKAEVKAHLSTIESTLAGTYQID
                     PATLLVVSTEEQPVTEGEEPAAPVSLLETPQLREQIQKIRERLDRMGAINLAAIDEHR
                     ELEERYQFLTTQEQDLSTSIASLKEIIQKINRTTKDMFVETFNELQQKFRDVFAQFFP
                     GGRAELQLVEEPLEEGVEDNGAREPGVEIVAQPPGKRLKSITMLSGGEKTLTAMALLI
                     ASFLIRPTPFCILDEIDAPLDEENIGRFTSVLRSLSSTAQFMVITHNKRTMAMADSLF
                     GVTMEEPGVSTLISVKLGDLQPA"
     gene            complement(31579..32541)
                     /gene="ispH"
                     /locus_tag="PK589_07340"
                     /gene_synonym="lytB"
     CDS             complement(31579..32541)
                     /gene="ispH"
                     /locus_tag="PK589_07340"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNJ19452.1"
                     /translation="MKIYLANPRGFCAGVDRAIDIVDLSLKKYGAPIYVRHEIVHSRH
                     VVNSLRHKGAVFVEELNEVPEGSVVIFSAHGVAKSVWEEAQSRRLHVIDATCPLVIKV
                     HNEVNRDYTQGYELILIGHAGHPEVIGTLGQIPDKFHLVSSVQDVETLHVEKTQNLSY
                     VTQTTLSVDECRDIVEALHRRFPNIKGPHQEDICYATQNRQNAVKALSKLVDVILVIG
                     SPNSSNSNRLRELGEHCGIPSYLIDAASDINPDWLKDAKSVGLSAGASAPEVLVTEVV
                     AYLKRLGSSEEVEELTVIEEDVEFLLPKELVTIESASRTAASAN"
     gene            32716..33432
                     /locus_tag="PK589_07345"
     CDS             32716..33432
                     /locus_tag="PK589_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ19453.1"
                     /translation="MSSPSSTHKKILIVEDEKDILQLVKLYLEKEGFRTVSAMTGTEG
                     LRQVKAEHPDLIILDLMLPELDGLEVCKRIRLNQETALLPILMLTAKAEESDTVIGLE
                     LGADDYVTKPFSPKALVARVKALLRRLDRNANSPQTQYHYGPLTVDLSRHEVRLEGEE
                     VTLTAKEFGLLEHLLRNVGRVLTRDVLLSAVWGYDYYGTTRTVDVHVRRLKQKLPLLD
                     DAIISIKSLGYKLRESDIPA"
     gene            33429..35288
                     /locus_tag="PK589_07350"
     CDS             33429..35288
                     /locus_tag="PK589_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ19454.1"
                     /translation="MNLSLRWKVALGTLAAVLVGLLVAGWLVIRSVEQTELSRMAEML
                     ETRSSLAAMALRPLFDQTGQAVPSPLLHTTIRELSQQARLRITVIKQDGTVLSDSAVP
                     AEGLTHIDNHLARPEVAQALASGRGMDIRASQTTGERTYYVARLLSEPARIQPGVPVI
                     RLGLPLTSIDERVRHIQQDLLTAFGAAFLLAMVLSLWVSRNLTKPLSEMAAAARQLAA
                     GTPGIRLTVSSSDEVGLLARTLNQMTDQLETKIKEVSDDRAQLLAMLIAMVEGVMVLD
                     YRGTVVQVNPALERMFALELTESRGRHYAELIRHEGLTALVSAVLQTRSGQGGEITLS
                     PSGSCLRVEASIAGGNREQEACAVFVFHDITELRRLEKIRKDFVANVSHELRTPLTSI
                     KGYVEALLDGGKDDPSTAAAFLEIIMRQSNRLNLILDDLLQLSQIESGQVLFRREPVE
                     LRALLERTVAVIKPLADKKHHTIELSLPDEYVVVEGDEERLVQVFINLLENAVKYTPD
                     QGRISMAIRQATQSRTASPRPMIEIVVADSGIGIPEADRPRVFERFYRVDKARSRELG
                     GTGLGLAIVKHIVEAHSGHVWVEGNAPRGSRFVVHLPVAQESSTTISTAAGNI"
     gene            complement(35254..36246)
                     /locus_tag="PK589_07355"
     CDS             complement(35254..36246)
                     /locus_tag="PK589_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNJ19455.1"
                     /translation="MAELSGLLLVVVILALLFDFSNGWHDSANAIATVVSTRVVSPST
                     AVLVAGALNVAGAFMSTAVAKMVGTGIVDPQSVTQTVVASALAGAIVWNFMTLMLGLP
                     TSSSHALIGGLVGAALTHGGMAAVKFSGLRSVLEAMILSPFFGFAIGLVLMIVLSWLF
                     FRVPRALALRLFSRLQLVSASFMAFSHGANDAQKAMGIITLALVSAGALPKAEVPTWV
                     IGSCALAMGLGTAVGGWRIVRTLGMRIVKLEPVHGFAAETGAAIVLMATAHIGLPVST
                     THTITSTVMGVGAVKRVSAVRWGVTRRILSAWLFTLPGAALLSTICYLLLSKLS"
     gene            complement(36311..36928)
                     /locus_tag="PK589_07360"
     CDS             complement(36311..36928)
                     /locus_tag="PK589_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNJ19456.1"
                     /translation="MFGLIPREEAFFDLFKNAAHNMIEGSRLLKDMTEDFRNPVEKAK
                     RIKDVEHVGDGITHDIARKLNQTFITPIDREDIHDLASALDDILDVVEAIADRFVLYK
                     VVAPTEAAIRLANVLYQAAVEVGATVDLLGKSHQAVTECSVRVNSLENEADRISRDAI
                     SSLFEKETDPIAVIKWKEIYENFEAGTDRCEDVANILERIALKHT"
     gene            complement(36953..37945)
                     /locus_tag="PK589_07365"
     CDS             complement(36953..37945)
                     /locus_tag="PK589_07365"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNJ19457.1"
                     /translation="MKCSRIVGTGSFVPPRVVHNEEVGKPLGLNSDQITALTGIRTRH
                     WAEPGQASSDLAVEAGRRACEAAGIPPQSLSAILVSTTSPDSAFPSTACHVQRALGAE
                     SVMAFDLSASCSGFLYGLSMADAMIRSGHIRSCLVVAAEVKSRSLDPADRETAVLFGD
                     GAGAVVLVQEESIRPQAAGILGVRLYAEGAGHGLITIPAGGSRKPSGMETVRGKDHLL
                     RMRGSAVFRAAIRHLEQAMLELLKEFGLSTVDVRRVIAHQANARILEQLRRRLGLPRE
                     VLYSVIERYGNTSSASLPIALDCAVREQRIAAGDVVVLAAFGGGLTWATGLVRW"
     gene            38147..39049
                     /gene="bamD"
                     /locus_tag="PK589_07370"
     CDS             38147..39049
                     /gene="bamD"
                     /locus_tag="PK589_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNJ19458.1"
                     /translation="MHRHSRDWCVRIAVMVGLLCFSAGCSSKPKTTAQGKPVSGTDEQ
                     IFLGDTIEKNYDPNVIMKRGEAFFDKEEFAEAIVEYQHFLELHRSHQLAVYAQFRLAE
                     SHLRMGKSIDRDPDPIQKAITAFEKLRKDYPNSKYEAQALQRLADCHDWLAKTHLFVG
                     QFYYRRSSYLAAAHRFDMIMKDYPDKTVAPEALYYLALTYQELGANDWAMEKLQLLAE
                     KYPNSDVAGSGRRLLAKLEKKSPPAAIVASKETPAPQSPPSNTLLAASVPSTFAAPAV
                     PELKRAPSAALSQPFVSCRLGAWC"
     gene            complement(39075..40280)
                     /locus_tag="PK589_07375"
     CDS             complement(39075..40280)
                     /locus_tag="PK589_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HNJ19459.1"
                     /translation="MADGYGPTTYDVIIVGMGPAGATAAAALSRGGLTVLGLDKDAHP
                     RYKVCGGGLSARIDHALEPGFHSVVEQSVTGVQFVYRGQDPLLIESARPIAYMVMRDR
                     FDHYLVQEAIQAGAHIRTGHGVVELMQDQHGVEVVTAQGRYRAQIVVGADGANSVVAR
                     RFFPQRSQYRAPALESEVPLGNGRHGHSASTILVDVGAARQGYGWIFPKRACLSVGVG
                     EFRRKSKSLRATFDRFVTDEQHVIGGAVPRPAGHPIPVYTESEKREQDEESRFVAGRA
                     ALVGDAAHLVDPLFGEGIYYAIRSGQLAAHAILAHRDDHHMAVSAYAAALRREILPDF
                     RVTARMAQVIYAFPRLAFKLLGRYQDVVQDYFEVLQGRTTAQQFLPNAKKRLKASVND
                     LLLEALHLR"
     gene            complement(40400..41146)
                     /locus_tag="PK589_07380"
     CDS             complement(40400..41146)
                     /locus_tag="PK589_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNJ19460.1"
                     /translation="MTDSVQSISLVAAFSAGLLSFVSPCVLPLVPSYISYITGLSIEQ
                     LTDVSERSRFRKAIILNSLLFIAGFSTVFVAFGASASLLGQVLITYQEHLRRFGGIVV
                     IVFGLYLLGILNLNFLKMEHRYQFRNRPAGFLGSFLIGIAFAAGWTPCVGPVLGTILL
                     YASTTESMFSGVVLLTFYSLGLALPLFLTALGVDRFLSYFKEVRSYLWGVSTVSGVLL
                     IVVGVMIYGNSLTMITSFLERYGIGWYLGQ"
     gene            complement(41148..41702)
                     /locus_tag="PK589_07385"
     CDS             complement(41148..41702)
                     /locus_tag="PK589_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNJ19461.1"
                     /translation="MMLSIIVGGLMIGGVMIADAPWASPLLSPAPAPILVASPAPQVD
                     EEAPNFLLKDSEGFNVTLDQFRGKVVLLNFWATWCGPCKIEMPAMERLYRSFSRKEFE
                     ILAVSTDAQGTAVTRPFQQEVGFTFPVLHDADFRIGLQYGARQLPMTFLIDRKGIIRN
                     RIPGARDWSAPAGKRLVEALLQGS"
     gene            complement(41722..42345)
                     /locus_tag="PK589_07390"
     CDS             complement(41722..42345)
                     /locus_tag="PK589_07390"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNJ19462.1"
                     /translation="MSDILNQSSSGVTTRSGPSRTVVVLAAAAILGVMFLVVWLQSSK
                     YEPLIVGKEAPDFQLPDLNDKPLRLSDYRGKVVFLNFWATWCKPCREEMPSMEVLYKN
                     FEKDGLVILAVSIDRVTTKKEIPPFVKGLNLTFPVLVDSWGQTDKRYKLMGVPETYII
                     DQQGVLREKVIGPRDWTRLDNLKVLTQLLKPAEKAASAATVEAVPAL"
     gene            complement(42349..43263)
                     /gene="ccsB"
                     /locus_tag="PK589_07395"
     CDS             complement(42349..43263)
                     /gene="ccsB"
                     /locus_tag="PK589_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HNJ19463.1"
                     /translation="MMRSLFLFDMTFWLYLAALGLYVAYLFAKRPAMQMAPAGHPIEN
                     LEDRDGAWATQLGQVATLVTVFGWVLNTMALMTRAFERMEHSGTFAPWSNQFEAMAYV
                     SWAIILGYVLLEFRYRIKAVGAFVVGIGFIAMGAASLLPYRYQTAEPLVPALNSYWIY
                     IHVSVTLTSYAAFAMAGGLGLMYLFKERAVNRGSQSRFYAAFPDLETIDELGYKAIML
                     GFPLLAFGIILGAMWANYAWGGYWSWDPKETWSLIVWLIYGAYIHARMTRGWEGHKAA
                     IYAIFGFLMVIFCFWGVNFLLSGLHAYA"
     gene            complement(43336..44772)
                     /locus_tag="PK589_07400"
     CDS             complement(43336..44772)
                     /locus_tag="PK589_07400"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNJ19464.1"
                     /translation="MNDKPDAASEMSASGPSTSGLGWEEFSREVVDFFASIKLAMFLF
                     IVLAMTATIGTVIQQGERPETYVQEYGEEAYRWFLRLGFTDVYHTWWFTGLLGLLCVN
                     SLTCFHKRFPSVWRSMRQDKVSVSLAFIQGMKQQTTLSLNQPKETVAESLVRLFAEKG
                     YKVLAKSDPGEVTVYATKGIMGRVGAHVAHLSATVIVLGGLLGSYYGFSEFGVCLEGQ
                     TYHIPRGNFDLRVDKFWIDYHENGSVKSYNSTLTVIDQGAPTMTKTITVNDPLVYKGI
                     WFYQSSYGDAWDQIEAARINIKDKASDKVVATVDLEWNKEKAVEGLPLKMKMTDFVAD
                     FAFNSTEKKVFSKTAEHSNPAIRLAVDERSTVQSTPWVFYHYPDLFEIKDSAYQFEFI
                     GYQPKKFTGLQIARNPGIMMVWIGCTMLVVGMTLSSLIYHRRLWARILPGESGVTVHL
                     GGTTHKSQIDFQKEFKKLTEKIQAKTSS"
     gene            45170..46318
                     /gene="metK"
                     /locus_tag="PK589_07405"
     CDS             45170..46318
                     /gene="metK"
                     /locus_tag="PK589_07405"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNJ19465.1"
                     /translation="MRHNYLFTSESVTEGHPDKIADQISDGILDAIIAQDKTSRVACE
                     TILTTGIAFVAGEISTKAYVEIPDIIRDVIKDVGYTDASWGFDSNTCSVLTSIHQQSS
                     DIAMGVDSGGAGDQGLMFGYATNETTELMPMPIVLAHRLTKRLAEVRKKKVLKWVRPD
                     GKSQVTVEYKDGKPFRIDTIVVSTQHSPDVTNKQIERDLMEYVIKPSMPKGLYDATSV
                     KHHINPTGRFVVGGPMGDTGLTGRKIIVDTYGGHGSHGGGAFSGKDPSKVDRSASYMA
                     RYIAKNIVAAGLASKCEVQLAYAIGVADPVSVLVDTKDTEKVAPESLDKLVRKHFPLT
                     PRGIIDHLKLRRPIFRKTAAYGHFGRNEPEFTWEKTDKAKALRKDAGL"
     gene            46425..47681
                     /gene="ahcY"
                     /locus_tag="PK589_07410"
     CDS             46425..47681
                     /gene="ahcY"
                     /locus_tag="PK589_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNJ19466.1"
                     /translation="MDYDVKDMGLADQGKLKIEWAEATMPVLRLIRKRFEKEQPFRGI
                     RATACLHVTTETANLMKTLKAGGADVRLCASNPLSTQDDVAAALVKHEGIPTFAVKGE
                     DNKTYYRHIESAIAHKPHLSMDDGADVVSHLHSKRKELLRHVIGGTEETTTGVIRLRS
                     MAEKKVLKFPVISVNDADTKHMFDNRYGTGQSTMDGIVRATNRLVCGSTLVVAGYGWC
                     GRGIATRARGMGANVIVTEIDPLKGLEAVMDGFRVMPMDEAAPLGDFFVTVTGNLKVI
                     RGEHFAAMKDGAIVCNSGHFNVELDIPALEKLSKKKLAVRSGVDQYTLKNGRRVSLLG
                     EGRLVNLATAEGHPSSVMDMSFANQALGAEFIVKNYKKLEKKVYPVPADIDKEISRLK
                     LAGMGVSIDKLTKEQVKYLASWEMGT"
     gene            48013..48750
                     /locus_tag="PK589_07415"
     CDS             48013..48750
                     /locus_tag="PK589_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNJ19467.1"
                     /translation="MATVRIGLSGYSYKPWQGPTRFYPPTLKQAQFLEYYSTRYRTVE
                     LDGVWFRLPTLSAVTKWIEQTPAHFVFSVKAHRTITHVKRLRPDAASFLHTMLEHLAP
                     LAEKQKLGPILLQLPPNFKRNDDRLAEFLQGLPPTYRWVMEFRHDSWNHATVENLLRR
                     FRVAWAAVETDDRPAEYRDTADFLYARLRRSQYSNKDLTRWGEYFLQTRRNGKDCYAY
                     CKHEDEGSPWMWADQLLKIMNVGTLPG"
     gene            complement(48739..50373)
                     /locus_tag="PK589_07420"
     CDS             complement(48739..50373)
                     /locus_tag="PK589_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ19468.1"
                     /translation="MLVFLIHVRDPQFYALPAKTRAKNGRIRVMGFPPIGIMSLSAVL
                     KRAGHECVMFDQAHPETPNEFIIEEIKRRKPALVGMSFLSTTSYPYAKILARQIRVAD
                     GSVKLAFGGVFASLNAGLVKLQCPEVDFVCRGDGEQLLLELLDHIDDPKDVAGVTWAK
                     DGKVINNPNRAMERHLDQWPFPDRESLELDFVESMPLDVPAVLSMERFTTMQTSRGCP
                     WPCVFCDIPIFNEGKWRARSAQHVVEELKFLEANGYGSVYFVDDHFLLQPKRIDAICN
                     GIIDAKLSIQWGIEGRVDSVAQHLFPAMAKAHCRTVMFGIESGSQKILDRLQKEQTLA
                     EVETAVRNAKKAGIEIVHGFFTVGNPDETVEDMEKTFDFASKLPLDTFGFNRLCVYRG
                     TPLWQEYIKRGLVSDAKDWYKYFKCSEIDPTCLPGATINSVRQEGLKKLFIYKLTRYP
                     IQTFKLLRRFLRYMPFRDVAYLIIKPFLGQKKGATKAEVLSRAVEHAEMKDAAAQLTQ
                     LSDELLHSVLEESKAERQRIQQEAESSRELPMISAR"
     gene            complement(50655..51527)
                     /gene="sucD"
                     /locus_tag="PK589_07425"
     CDS             complement(50655..51527)
                     /gene="sucD"
                     /locus_tag="PK589_07425"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNJ19469.1"
                     /translation="MSILVNKNTRVVVQGITGKEGSFHASQCKAYGTKMVAGVTPGKA
                     GQEVEGIPVFNTVADAVKKTDCDTSLIFVPPPFCADAILEAADAGVKLIICITEGIPV
                     NDMVKVKRALHGRDVRLIGPNCPGVITVDEAKIGIMPGFIHKRGVVGVVSRSGTLTYE
                     AVHQLSTLGLGETTCVGIGGDPVNGTGFVDVLPLFEKDPETQAVVMIGEIGGDAEEKA
                     AEYIKQHVKKPVISFIAGITAPPGRRMGHAGAIISGGKGTATEKMKALESAGVRVVKN
                     PAEIGHAVMKALGR"
     gene            complement(51539..52717)
                     /gene="sucC"
                     /locus_tag="PK589_07430"
     CDS             complement(51539..52717)
                     /gene="sucC"
                     /locus_tag="PK589_07430"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNJ19470.1"
                     /translation="MNVHEFQAKQLFAQFGVPVPRGKEITSPEGATAWANELNTPVFV
                     VKAQIHAGGRGKAGGVKITKDRGAIAGLAKELIGKTLITHQTGPKGRTVHRLLLEEGA
                     NIAKELYLSILVDRDTGWPTFIASTEGGMEIEEVAEKTPEKIIKEAIDPAVGFQGHNG
                     RNVAFALGLQSMEPAVINPFVQMLGNLYRLFMEKHAALVEINPLIITKEKTLVALDGK
                     VSFDDNGIFKHEDVQKMRDLNEEDPLEIEATANNLNYVKLDGNIGCMVNGAGLAMATM
                     DVIKLAGSEPANFLDVGGGATKDTVAAGFRILLKDPNVKGIFINIFGGIVRCERIAHG
                     VIEAAKEVKITVPLVVRLQGTNADEGRKLLAESGLKLEVANDLWEAAQKIVKLTGKAA
                     "
     gene            complement(52803..54404)
                     /locus_tag="PK589_07435"
     CDS             complement(52803..54404)
                     /locus_tag="PK589_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNJ19471.1"
                     /translation="MDIHALQQIVHQTRDARRKQTIPKFSPADRDQLIHKYHPDYRAS
                     AYRPIRFGPNADDKTVVELAALLEGDSSIPEHLDLTPQYTADVLVIGGGGAGCAAALH
                     AHTSGAKVILATKLRLGDSNSVMAQGGMQISVAPEDSPVTHFLDTLKGGHMQNDHELL
                     KVMVEEGPSIAKWLIELGVLFDRQADGNLHVKKGGGSSKPRLLTCSDYTGLEIMRVLK
                     DEVLNQKIQLLEFCAAVELLSDDNGQCTGAIFKDLDNHRHVIVAAKSVILATGGIGRL
                     HIQGFPTSNHYGATGDGLCLSYRMGAKLDHIDTFQYHPSGAVYPEQLIGALVTEGIRS
                     EGGHLVNAKGERFVNELDTRDVVSSSIIRECEEGRGIRTMSGRIGVWLDTPLLDAEHG
                     PGTVEKHFPAMMMQFERFGIDISKDPVLIYPTLHYQNGGVKIDANSETNVKNLFVAGE
                     ASGGLHGRNRLMGNSLLDLMVFGKRSGLTAASRAGSMSQGKLTLNHLKRFRAEAKKHG
                     NSSGVISPMILPAYTRKEPERTATK"
     gene            complement(54473..55183)
                     /locus_tag="PK589_07440"
     CDS             complement(54473..55183)
                     /locus_tag="PK589_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNJ19472.1"
                     /translation="MAQEDTNVIDQPEVLRHKMVTIEISGKKYEVPEGITVIKALWHT
                     GQEVVRGAGCLGGFCGACATYYRTKDDPKVRTCLACQMAVEDGMSFTIMPPFPARKAL
                     YDIQSLKDPKQDLFNLYPEAPLCRNCNACTEACPQKIDVREGVWKAVFGDFKSVSEMF
                     MDCVMCGMCTPVCIADIAPNLVALYVSRAQGAHFTEKPQGLDLRIKEIQDGRFNDEWN
                     KVLAMNEQELADHCATVK"
     gene            complement(55267..>55571)
                     /locus_tag="PK589_07445"
     CDS             complement(55267..>55571)
                     /locus_tag="PK589_07445"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNJ19473.1"
                     /translation="ESNKSPARKVGEIDNRGSHFYLALYWAQALAAQTADKKIAERFA
                     KIAKDLSDNEKKIDGELLAAQGKPQDVGGYYHPNDAKAAKALRPSATLNAIIDAIA"
BASE COUNT        12844 a        16744 c        14349 g        11634 t
ORIGIN      
        1 agcctgccga agcaaccggc tcgtcaggtc tccgcctata atctgatccg cataccgccc
       61 gaaataacca agccccactc cggggccgca ggcgacttcc agcacccgtc ggcctcgaca
      121 gaagtccctt gcgaagcgat agcgcgtata gaccatcgac aacgcttcgg gggttaccca
      181 atcgccggct ttttctgtca cgggggtgta atcggagagg atcacggctg cgtggagaaa
      241 aacgcccgga gtgcagcgac aatctgttcg acttgcgcct ctgtggtttc agcactcatc
      301 ggcaacgaca caacttcccg gcagatcgca agcgtttcgg gaacatccgg aaccagcagt
      361 cccaatcctt tgtgttccca catgggtttc tgccaatgga ccaatgtctc gaccccttgt
      421 tccttcagat aggcgcgcaa tggctcgcga acggtcgtgc ggatcacata gttctggaag
      481 atgtcctggc gccgcgactc gtcgaattga ggaatgcgca gttggctaat tcccgccagc
      541 ccgcgatgat aggcagcggc aatttgccgc cgatgtgcga tccactcggg cagatgacgc
      601 aacttcacat ccagcacggc agcctgaacg ttatccaaca aggccgtctg cccatgatag
      661 tgatattcac cggtcgcgcg gtcctccccg ttataacgaa ggagtgtcgc catgcgggcg
      721 agtgacgcat cgttcgtggt aatggccccc ccgtctccga atccaccgag gaccttgaag
      781 ggataaaaac taaaacagcc cgcgcgcccc tggcttccgg cacacttgcc gtcgaacgtc
      841 gccccgagcg cctgtgccgc atcctcgatc acggccaatc catgtttgtc ggccagcgct
      901 aagatacgct ccatgtcaca gacccgtccg ttcagatgca caggaagcag cgcttttgtc
      961 tttggcgtga tggcccgctc aatgagatcg acgtccatat taaagtcagg accgacatcg
     1021 atcaacaccg gctgcgcccc gcaatgcacg atcgccgaca ccgttgcgac gaatgtatgc
     1081 gctaccgtaa tgacttcatg acccgccccg actccggcgc caagcagtgc aaacaggagc
     1141 gcatggtacc cgctgttcac cccgacggcg tacttcaccc cgacaaactc ggcgaggtgc
     1201 tgctcgaaat ctttcagttg atgccggttg acgagatcgc cgtttgccag gcaatcgatg
     1261 atcgcccgat cgatctcggg cttgagtttc gcgtagtgac tgcgcgggtc gataaaggga
     1321 acgtgatagg ccataactac gatctggtac tcgacacaag cactttttcc acagtggaca
     1381 caatctcttc cgcgccaatg tagtacgctt cttcaagcgc ggcagacgtc ggcgcaggcg
     1441 catccggcaa acagacgcgg ccgatgggcg ctttcaaagc atgaaagact tcccctgcca
     1501 ctgaggcggc aatttccgcc gcgatgccgc cactcatcca cgcggcatct gcgatgacca
     1561 agcggccggt cttgccgacc gacgaataca ccagattgcg atcccagggt ttaatcgaac
     1621 gcacatcgat caattccaca tcaatgcccc gctgggtcaa cgtctcaacg gccttggtgg
     1681 cttctttcga catgtatgac gtggcgagca ccgtcacatc ccttccactc cgtcgaagcg
     1741 ccgcggagcc tatgggcacc tcatacagac cttcaggcac atccccctgc agatcgtaga
     1801 gccatcgatc gtcgatgtag agcacgggat tgccgtcccg gacggccgcg atgagcagcc
     1861 ccttcgcatc atagggagtg gccggcatga cgaccttcag cccgggcaca tgcgcaaaga
     1921 gggcctgcag agcctgagca tgttgcgcgc cctgttcgcc ccctcgatta atcactgctc
     1981 gtatcacgat cggcacggac acccggccgg cgaagagata cgaccaatta gccgcctggt
     2041 tcatgatggg gtccatcgcc agcaacataa aatccatgcg cggatgaaac acaatcgggc
     2101 gcatcccggt gatagcagcg ccgatcgccg ccccggtcgt ggcattctcc gacacgggcg
     2161 aatcaatcac gcgatgggtc ccgaattcct tgtccagatc ctgcaggctg gttcccgcat
     2221 accagggatt gcgaacgccc tgtccaatca agaacacccg tgggtcctct gcgaggagtt
     2281 gggcgtgagc ctctcgaatg gcctgcgcat acgtgagtat ccgcatacgc ctagactccc
     2341 ccccgcgcgt aaacatgctg tagtaattcg ctttcatccg gggccggcga tagccgtgca
     2401 aagctcacgg cctcgtcaat ttcccttctg atctcggcct ccagccgatc aaagacttct
     2461 gacgacaaac catgtacctt cagcaagcca cggcactgcg cgatcggatc gcggcgctgc
     2521 cagtcatcaa gttcgcgccg tcggtccgta ccaacctcga gatcggccgc aggccccaca
     2581 tgtccgcgcc accgatacgt gcgacactcc agcagcgtgg ggccctcgcc aattctcgcg
     2641 cgctgcacgg cctgatgcgc cgtctgatag accgccgtcg catcgttccc gtccaccacc
     2701 acgccgggca atccatgcag cgctgcgctc tccacgatat tgtcctttac ccgtcgctct
     2761 ttcagcgtca gatgcgtgga gtacaaatta ttttcacata cgaacaagac tggaagacgg
     2821 tacaaggctg ccagattgag cgactcgtgg aagtgcccct cgtcggtcgc accatctccg
     2881 aaaaaagcca cggccacctg ctgcgttcct cgcaatttgg ccgaaagcgc ggcaccaacg
     2941 gccagcggaa tggtggccgc caccaacgga accgtgccaa agatgccctg ggccggatcc
     3001 accagatgca tggaacctcc acgtccgcgc gcgcaacctg tagctttgcc aaagatctcc
     3061 gccatcatgc cgcgcagatc gccgcccttc gcaagatagt gcccatggga ccgatgcccg
     3121 ccccacagag tgtcgtcttg ctgcaacgcc gcgcagacac cggcgggaat tgcttcctgc
     3181 ccgatcgaga gatggcacgg tgtccgcact tcgcgagttt cgacgagttc cccaatgcgc
     3241 tcttcagcca tgcggatgcg ctgcatggct cgaagcaacg atatgagttg gtctcgcgac
     3301 aacgcacctg tatgcacatc cgggaagttc atgcacctaa ctccagcact cgtggttcgc
     3361 ggaatttcac acggaacaga accgctccaa cggtccggag cagaatatcg agatcggtcc
     3421 ggaagccggc ctggcgcaca tacgcgagtt gaaggcggat tttcttcggt ctgatgagct
     3481 cacaataggt tcgatcagga tcagcactcc cctcaagaat ggctccctca tcgatgttcc
     3541 agagactcgc ccaatcggtg agaccgggcc gcactgcgag aatggctttt tcttcgtctg
     3601 tgtacaaatc cacaaatcgc tgcacttccg gccggggccc gacaagactc atttcgccac
     3661 agagcacatt ccagagttgc gggagttcgt ccaatttata tcgtcttaac accgccccga
     3721 cgctcgtcac ccgaggatcg tccttggctg ttgaggtcac gccggaccgt tcggcatccg
     3781 atctcatcgt gcggaatttc aaaatacgga atggccgccc gtttctcccc acacgcaacc
     3841 cacgataaaa cggtgagccc ccatcgccca atgtgatcaa cagtgcaatc actaccagca
     3901 tgggagaaag cagcattagc cccaggaccg cacaaagaac atcaaatact cgtttcataa
     3961 tagaaaagac gctaccgccc actgcttcag tgtcaagaat cgatcaacca gaatcctgct
     4021 acacgcgcat gctcagtaac gtgccagtat ggcggctgag atggtaccgc ttcaagagtg
     4081 tttttcaggc ccaacgcgct ctcctcatga tttgcccact gcaaaccgag ccatccatga
     4141 tcgccacttg taccccacac gctgagcaga actgcattcg gggccagaag tgccttgaga
     4201 acggaataat caacggaaga tgttgaaagg aagcgctacc tgcgtggcaa ggtacaagaa
     4261 ttgcccgacc tgtatttccg aaacatgatg gaatccagaa aaacttcctc cacgtctgtt
     4321 ttatctggcg ggggcggcac ggtttgtctc cacattgaat attttctgcc aaaattcacg
     4381 gattgtaaga agcctgacca ccccccagag ggagccgagc ccgtatccga catgcaacat
     4441 cgcaaaaatt atcggcatca gcactgcgta ttgtggttgc cttgcctccc aggcgacatg
     4501 gagtgatgcc accacattcg cgagcagata ggcgccgaga atgcccgcaa acatccatcc
     4561 gaacgtcccc tgccacgcgg ttaagacgag cgtagatacc aaactaccga caaaaaacag
     4621 tggaacgagg tgacgtaggc taaccggaat cacattgcta tacgcgaagg gcaaaatagc
     4681 ccaaacgcca ttgatccaat tgtgacacca gaaggacttg atgtctgacc gagcgtaata
     4741 gtaactgacg atatcaggta tcagaagggt gcggcctccg gccttccgca aacgaagact
     4801 gaattccatg tcttgccctc gcgtcagccg ctcattgaac acgcccggtt gattcaaccg
     4861 aaggctaaac tccatgtctt cgccccggac caggctttca ttaaatccac cgagttcctg
     4921 aaatacccat cgtcgcacgc aaaagaacgg aaccgtatcg acaaacttcg gctcgctacc
     4981 ttggggcagc cgaaaatgag aattgccaac cccgaatgga tgggacagag ccttaacgac
     5041 gccccatccc gccaatgtct ccgttcgagg cagcgttttc caaattcctc ccacattgtc
     5101 gactttgtac tttcccagag catccacgca gcgggagatg taatggctgt catatcgagc
     5161 atgggcgtcc atccgcatca caatggggcc acgggtgtgt ttaatcccga ggttcaaggc
     5221 agcgggagtc acacgtttgg ggttgtccag caccgaaagg aacgcatggt cacgggaaaa
     5281 gccgtcaaga atttcacgcg tgccatcatc gctcaggccg tcaaccacga gtatttccaa
     5341 gcgatctttg ggaaaatcat ttgcgagcac ggattcgagg cagccagcga tgaacgccct
     5401 ctcatttcgg cagggaatga taatcgagac gatagggtat gtgtgaaaaa acggaccaga
     5461 gacccatggc gtttcagcct gtgagttagt gaaactcatg cgcgtgcacc aatcagttcc
     5521 tatggcctag tttggaggag ccgccgcttc ccgataccga taaagcgatc gattgacccg
     5581 aaactcctct aggcgcttca ccaacatttc accgactagt ttgtggccct cttcactcca
     5641 atgggcgccg tcagcatgaa acacatctct tccttgacgc gccgcaaagt cgaccgcctc
     5701 ggcaatctca tcgagaaaga tgatgtcatg tcggcctgaa atattcttca gtgcagcatt
     5761 atagggctct accgctttgc aattgaaggc cactactggc gtggctccaa cacgagctct
     5821 cacagttgcc atcaattcat ccgttgtacg aaccgccctg cgaaaatctg gatgctgcgc
     5881 cccggatcgc aagatttcag cctctacgct atcgagcaga taggccgcat agagccgatc
     5941 cattcggctc aacaaaaaaa caataaaccg tgaatactta taagcgagcc cccgaaattt
     6001 ccccagaata gaactcggca tgtcacgtat gatttccccc ttgctccaat acgggcgttg
     6061 acgaaaatct atatggagca tcgtgcgacg ctccatcagg ggatcattat tcgcgaaatc
     6121 gttgtagcaa tattgccaca caagcagatc cggcttgatg agatcaaaaa accgatccag
     6181 gatcatatac tcttgcagag tgccatagtt ttccactcca tacgcaaata cttcgtaagg
     6241 acgttcgcgt ccaagcaaag catagtaagt cttcgaatca gaaacttctc gcgcatgcgt
     6301 aaatgaatcg ccaagtatca atatcttaaa tttgtccgac ccaatctcac caaattggcg
     6361 aaacccatag cggttctgcg acagcgtcac aacatacgac ttgccttgct tagttttttc
     6421 cgccctgttc gcgcggtagt tttcagtagc cttccagccg agtgtctcgt ccaacatcgt
     6481 gccatcgaaa ttccccagaa aaaaacggtg ccattcgcga gcaggaattc catctttcgt
     6541 ccaatagtaa actcggaaca cggcttcacc acacaatatg gcgaaacaaa ttgagacgag
     6601 gggaaggagg cacgtcgcga tagacttaag tagctggaca tgtcgcgaag tcattgcgat
     6661 tgggattgat ggcggctaag tctcaaccgt gatgaatcta gacttgaatc tgggcgcact
     6721 tcttttgcca taagcacgat gtagagtccg aaagaaatca gggccaatgt tgtacttgta
     6781 taggtgaaaa ggctgaaact aaaactaatc atgaccgcag catatactgc tgtcagaaaa
     6841 ggaaccgcga ctaccttgcc tctcaacact tggtggtatg aatgtccggc gatataacca
     6901 aaaattaaag ccgagaccag agcaccgatg tggccaaaat catcgatcaa tccttgaaac
     6961 accgtataaa cgttcgagct cgtgtagcca acgtcaacgg cttcatatat gtagtgatcc
     7021 tctgtgccta atctggcaaa ctcgaagatg cgcccgaatg tgcgatctcc atatgtcaga
     7081 tcagaaaaac taaaagtccc cttatcgagc aagatcgcaa atgagggaag aaacgaaaag
     7141 atatctgaag cggccaggag caacaacgat agtgttgaga cttcttcatc accataacga
     7201 agggagagta ccactaggga cgtccccact attgccaccg caatacagat gagtgctctt
     7261 aaattgctca catcactatg cgcctttcca ctccgacctc ccagcaacct ggctgccata
     7321 tatgccgaaa tccagaacgc acctccgaac atggccccca tacgcgagcc atagagcaaa
     7381 ttgtgcgccg caggggcgat gacagcaagg acggcgatag ccttgtccat attcgagaca
     7441 gcaacctgaa acagaattcc cccaaacaac ggagccgcat agaatgcgac aaataatact
     7501 ctttctatga acgtgagctt ctccgatcca tacacaaagg tcgcccgatt ttgcatcact
     7561 atctggctga ggttctccac agaaagcgcc tcccccaggc tgggactccc atccagaata
     7621 atgtagctga ttctcactaa accgataaaa accagagtgc ccgtgacggc acgtaaatat
     7681 ggatatatga atcgcttgtc ttcgcactga ttttcaacgc gctgcgatcc tgttccccag
     7741 aggcatccaa gatgcatcaa aacgccggaa ccagcgatcc atacgattcc gagaataagg
     7801 gtttcggaag gcacgcggaa taacaacgcg gtgagactgt accaactcca aagcagcgcg
     7861 aataaagcgc cgggggataa ccaactgccc tccgcaattc gggcaatcaa tgcaatacag
     7921 gctaatatca agactccttg cacaaataag aacatctgtc ttacctaacg aacattttga
     7981 cggtcatcca gacacatgag aactttcagt acttcagccg cgtctatcga gcgcatacat
     8041 tcttgaccat gcggacattt gccatagagc acggtctggt agcatggcat acagtccgga
     8101 cgtttttctg tctgcacaac acgatgcttg ttgccccagg gacgggtatt aacgatgtcg
     8161 gtcggccctg cgacagtcac gacaggcgtt ccaacggctg cagctatatg ccccaaacct
     8221 gtgtctccag aaagaaccgc ctgacacttg ccaatggttg ctgccacgac cgtcaaccgc
     8281 aacccagaca gaatgatcac tcgctcaagg ccccagtcat acgatttgat gagaccggtc
     8341 tcatcagggc cgaaaaacaa aacgactctc cgggtctcat ctcttgctag atattcctgc
     8401 acaacctctc gacaaagttc ttgtggaatc tgtttgtccg gacattcact gccttgacca
     8461 ccaggatgga cgcccagtac gtcgcatgta tctaggtgat gattcgtcca aatcctggcc
     8521 gcctcagttc gagcttgttc atcgaactca aagtgtagat ccccaattcc acaattcggt
     8581 aatgcgagct tgagaatctc cgcattttcc atcaccctgt gattcgtatt gtcgactcga
     8641 cgccaatggg tatatcccca cccttttgcg ccacccccat caccgacaac ggtccttacc
     8701 cctgagagaa atttcagccc aagcgcgatc ttcggtgaca gcccgcttgc gatgaatgct
     8761 acatcgaatt gctgcatgcg aaggcgaaca aagaaccgca gaatcgtaaa catcgaactg
     8821 cgtcgaaacg acatgacttg cacttcctca cacaacctcg aacctcggat gacctcagct
     8881 gctgaatctg acccggccac tacacagata tgtgcggacg gttgcgctag acgcagtgca
     8941 tgaaccatgg gcgtggtcat caccatatcc ccgattcccc atgtctgaac gacaagaatc
     9001 ttcttcattg aacgttatcg cacaatcgca acattccaca ttgacctaac cggccgcact
     9061 agccaccagg tcaacaagcc atacgagcag atgtacagca caccgccccc agttgcctgc
     9121 catccatggt ccacaggcat caccagaaac gatgtcgtgg cgaacagagc gcaagtagcg
     9181 cacttcatgg aaaaccaagc cagatccttc caaataggag agttcagtcg ccagccaaga
     9241 ctgatgactt gcatggcgct atctacagcg aacgcgagtg caagactcag tgcaacgcca
     9301 gctaaaccca acgccggagt caacattaga cacaatggca cgactaaaca ggcgcttaga
     9361 atcgttgcaa gcagcggcga ccgggcatct cccgctgcat acagggcccg agtaccaacc
     9421 ggttcccagg ctccaaaggt gaggcctatt gcaagaaacc caaaagccgt cgccgtgagc
     9481 gtaagcgctg cctcatcgaa tgcaccccgt tggtagagca atcgcaagag aggtttttga
     9541 tatacccaac acaacactga gagcggaaga aatccataca ggattaatct ggccagccct
     9601 gcaacaaact ctctgaattg ctcttctcga ccgtacgtaa acagggaggc gaaggtaggg
     9661 tatgcagccc gcgccacact caccgtaatg agcgaaatca gtcccgcgac gatggtgcgt
     9721 gcatagctaa atgccgcgat gctgccttct cccaagccag aggcaaagta gtttgccaat
     9781 agccccggga actgagaggc gacgtagtac aaggcaacag gcaggcaggc caatattgga
     9841 tgtaccaatc ctgtaaagga cagcgcacca aagtgtgcgc catgcgttct cagaatgata
     9901 cccatcagca cgaggaggca aatgaaaatg ccccccagat tcccgatgac gagggcatgc
     9961 acacccacgt tagaggctag tgccatgaga accccaatta cgacgacatt gaagagcaaa
    10021 tcactcacgg ccggcaatcg aaaatggttg aacgcatgaa atacggcaga taaaacatag
    10081 acacaattct gaataagtac gtaaatcgag agccacccca taagctcgac agtgagtctt
    10141 atcgttgcgg cagacattga tggtgcggcg aacttaacta aatactcagg tgctccccag
    10201 accggactca gcaaaatgaa agaccacgcc acggatgaga cgcaactcgt actcaaatag
    10261 tgaagacccg cctgcttccc atgctccgcc accatggagt ggtaccctgg gataatggca
    10321 gtagaaaagg cataaccaat cccgcccgcg agaaaaaggg gaatagcata ggcaacagca
    10381 taggcatcga tctctctgcc agccccaaag tagctggcca gcaagagttc ccgcacaaaa
    10441 cccaaaacct tgctgattcc ggtcacccca aacaggagtg ctgaagctga agtccaagtc
    10501 tgaaccgaaa gcgctgttcg tgactcatgt cccatactca tctgtggagt ccctacgaat
    10561 tacatggcga aacctaactt gggacatacc gattgaagct gcttatattg attgattgac
    10621 gccacgcctg ccaaattctt atcatcggcc cccacgcgtg aaaccactgc tctcccatcg
    10681 ctttcccatc agcccactat ggtcagggct gcttctcgcg ctgctctacc tgaatgtcca
    10741 cgacgtgctt cgagctgagg aatctgcctc ccccaccacc tcccacgtcg tcgacatgcg
    10801 gttctccacg acgacgtttc tactcaatct tgaaaaacgg ggactggagg ataatacctt
    10861 caccaacggc ctgggacgcg acacgacgtt gatcgggaac ctgatcaacg cgacgctcta
    10921 caaacgtcta cttccctcgc tagaggccga aatcggcgtc tttgccaaca tgccctttgg
    10981 acacgatcac gaaatctcac aggtccgtcc cgtcgtccgc ctgacctatc aaccggtcga
    11041 gcaaatcaca gcagtcgccg gcacgattcg caccccgcac cgggacttcc acgacgcagt
    11101 gtttgacaac ggcaaccggt tcctgcgccc gattgaacag ggggctcaaa tgctagtgga
    11161 ttccacccac tatcagcaag acctgttcat caactgggag caggcctttc gtgggtcggc
    11221 ccccaatcga tacgatgtcg ggtatgccgg gcaactgaag ttcggccctt tgcatttcaa
    11281 tggtcaggcc cattgggtgc gcaacgggca ggcactgttc aagctagacc gatcgttttc
    11341 gacacgcgac aatctcgtcg tcgccacggg tccagaattg gtgcttgaac catcccgatg
    11401 gttcgcatct actgcttggt ggcatcagat tggtatcagt ttcacctacc tgactagcta
    11461 caaccaggcc gacaatggct cagacgcgtc gcgcgggcgg ggttatgaat tacgcacctg
    11521 gctcgacttg gccggatggc gtccctacct cgccttttgg aagggccgga acttcctcag
    11581 ccaacaggga gacccggaat ttagagcgag caatttcccg gaattcgggc tctcgaaaac
    11641 gatccgtctc ggcaagcgcg cctccatcga attcggagcg caagcgcgcc gtatccgagc
    11701 cttcttcacc gaagacggcc tcaactatat gaacagggat acgtttaagt gggtgaatca
    11761 ggaatatctc gtcttcaatt gggattggga taccaaccac gccgacctga ttggtgatgt
    11821 atgggcttcg ctgtctcggc cttttgatga tcgacaggac gctccgtcag agcgcaggtt
    11881 cacagccaaa ctcgatacac tgacctatgt ttacagtatc tcctatcacg gggtacggca
    11941 gatcggcggc atcccggtct caaatcacac gttcgcggga caatatctct cgccggtact
    12001 tcaatatcga gtcagccccc aactcacact cgacgccggc ctgttcgccg gattgcccgt
    12061 tggagataca cagtcctttc acaccgtgca accgattctc tccgcacaat atgaaatggt
    12121 gccggatgtg cgactggtgg ccggaaccct gcatcgcaac catcctttcg tggacgcatt
    12181 attcaatgat gccacgctgt tcagccgccc cgtggaacaa ggcttccaat tgctggttga
    12241 tcgtgaacat taccagcagg atctcttcat caactggaac cagatcgaaa cgtatcagaa
    12301 ggcagagcgg ttcgacgtgg gctatgcagg tcgagtagca gcagggccct tcgcgctgaa
    12361 tggccaggta tattggaccc atttcggtgg cgcccaatat tcagaagccc gcacgttttt
    12421 cggccctgga ctgccccgcg accgccccgc cggcaacaat ttcctcacgg cgttcggtcc
    12481 tcaattcacc tggcagccta agcagtactg gccgcagttg tcgtggtttc gggaaatcga
    12541 tgtcatggcc ctgtatctga ccagtcagaa cgaaccgacg cagagcagtt caccaatcgt
    12601 tcgtgggcgc gggtatcaac tgacagcagg cgtgaacttg gacgggtggc ggccctatat
    12661 cacgctctgg cgcggggaac actttgtcac cgagcggggc gacccagcct actatgcggg
    12721 tgacttcaca gaatttggcc tgttcaaaga tttcatccta cccgccggct tttctttacg
    12781 actcggcgga ttcggccgaa cgatcgaacg gcatctcacc catacggagt acgcgctgtt
    12841 gaattggtcg tgggatcaaa cgccctggcg cggtttctgt caacgcccca cgctgctaca
    12901 ccgaagtgag tcatcatgcg gtcaatcttg agatacactt ctctcttgcc accccgcgcc
    12961 aagctcgtta gagtagcact ttagtgctcc tgaggccgtc agctattgta agaggagggc
    13021 gcatgcgccg ttcgtcaatc ggtcttctgt ttctggccct gttgtgcatt ccagccgtca
    13081 cacgcgccga gaactatgtg gctgcgggga tcggcgtgaa tgctcccagt tttgatacca
    13141 cggatgccgg caaagtctct ctacagaagg atctgtttta tggaggcaaa atcggtcact
    13201 actttaatga ccggggctat aattggtttg gccttgaagt agacatgtac cggtccacgc
    13261 ccggcatcaa gcaacagacc ctcccgacct ccagcaatcc acgactgtcg gggccaatgc
    13321 cgggcgctga tttgcaggtg cattcgttgg cctttaatgc actagtccga gtcaccggct
    13381 atcagtacaa agtggagccc tatgccggtc ttggcattgg cctgaacgta ggcaatattt
    13441 ccaacggcaa cttccgacct gaagcatcct tcgctccaag ctttaatgtc ctcgccggta
    13501 tccgttacta catgactgag tcaattgccc ctttcattga gtacaagtat aactttgctc
    13561 aattcaagtt cgaccgcagc aatgtcacgg cagactatcg agccaatctg tttatgttcg
    13621 gcattgccta ccatttcgga cggtaacccc tgccggatcc agcgtcctgt tcttacgagg
    13681 acgacctctt ctttccatcc gatggtccgc caacgatcgt gagaacagtc agcactattc
    13741 tccgtcggca tcggacgcgc ggaagtgttg cccggacttg atataggcaa gaatgtcggg
    13801 cttcggcacc ttcaacgcct gaaatgcatg ccgaatcagg gtatgtgccc cttcgatttc
    13861 tggcaggatg atttctgtcg cgcctaccgc cctcaaatct tctgcctccc taaatccatg
    13921 tgcccggaca agcaggggta ttttttcatt cagtccccgc agccgtcgaa cagtcagcga
    13981 tgcttcgtgg atcaccggga gcgcaacaat caccagagca gcccgctcag cgccggctgc
    14041 agtcaacagc tcggtttgcg aggcatctcc atacacgcag gaaatgccgc gacgccgtaa
    14101 ttgtcgtacg aggtccgggt cgcgctcgat caccgtgtag gcgatcccaa aatggtcaag
    14161 ggccaggccc accgtgctgc ccatgcgacc gaatccgcac accaccacgt gtcctgcttg
    14221 ctcgtcttgg acgccccctt gcattggcat ggcagcccgt cccattcgca agtgcaaggc
    14281 cgcgacccac ctcggtgcat atcgaacgaa cggtgcgttg agaagaatgc tcaacaacga
    14341 tgcggcgagg gtggcattat acacgtcctc tcccacatga cctgccgtct tggcgacctg
    14401 caccaacaca aaagaaaact cgccgatctg cgtgagaccg acgccgacga ggagtgcggt
    14461 gccccaggcg taacggaaaa gtcgtacaac cagggtccag atgacgaact tgccgatgag
    14521 aatcaatccg agcatgaggc cgagcagcgg aagattggac cacacgatac gcgggtcgat
    14581 cagaatcccg atcgtcacaa aaaagagggc gacgaatgca tcgcgaagtg agagcagcct
    14641 ggcaagcgtt tcgtgcccgt actccgaccc gcttatgatc aacccagcca gaaatgcgcc
    14701 aagggcaagg gacaatcccg ccatctgtgt taccgccgcc gtcccgatgc ccagagatag
    14761 cgagaccagg agaaagagct cggcattttg tgtcttcgca atgcgcgtca ataacggagg
    14821 gatcgctttg gccgcgagat aggaaaacgg aaccagaatt agaactgcca accataacgc
    14881 ctttccaatg gtagcgaagc gacccggttc cagcgtgccc agcgctggaa gcagcacggt
    14941 gaggaccacg acggccagat cctccaccaa tgtgattccg atcatgaccc tgccatgttt
    15001 ggcccgtaag tcgccgctat ccagaagcaa ccgcgccaac accatcgtac tggccacgga
    15061 gaccaccgac ccaatcacca cgctttggat catggtccat cccaccagat ggccgagccc
    15121 gacggagaga agaatggaga ggaaaatccc gagtggtcct cccaggaggg cgacccaacg
    15181 aactcgcaac aagtcattca acgaaaattc gataccaatg ctgaacatca acaaaatcac
    15241 gccgatctcg gcgaacaatt caaagctgtg gacatctgag atggatgggc caggcgtcaa
    15301 gggactgatg agaatgccgc cgaagacata tcccagaatc aatggctggc gaagcagata
    15361 ggcggaagtc cctccgacga ccgcagcgat gaacacatag gcgagatctc gaaaaaaaat
    15421 tggatcaggg gtcatgcatc ctttcagaca acaaattcgt catccgcaaa ttggtctgag
    15481 catcaaacag gccttccgta ttctaaccat ttcacaattc aggaggtaag gttcttgatc
    15541 caaaagggca cggctttggc ttctcacgca cgcgccaaca atgacatcga cagcctcaag
    15601 tttttctctt ttgcgcccga ccgaacagcc ctcttgcctt acactatcca gtgtcaaatg
    15661 ctgacatccg aacggtgcag taactcatgg gccattccaa gagcgccatg acgaggcatt
    15721 acgcccatct cctccccgac tcatttacga agaacgatga atcgagcgaa tgtgggtttg
    15781 cagcaaaaag tgtgaatgga ctcgggagta aaaccgggaa gtaacgaaac tcagcgggtg
    15841 gaacagggtc cggtcagaat caccgaaccc tctgagatat ctgcaggaat ggttggaggc
    15901 gccgggcggg ttcgaaccgc cgcatcgcag ttttgcagac tgctccctta gccacttggg
    15961 tacggcgcca cgggtcggat tatagaaggg ggaggttcag gaactcaacc gcgaagagca
    16021 gagaaacaga ggaaacttcc acaacacagc cgctgaacga atgccgcgga ttacggctta
    16081 ctgggaagca ctggaatgtc gcgaacgatg gtggattgtt catccggcgc atggcccatc
    16141 aattcccaac cttcttcggt ctgtccgtcc gacgacccgt tgtgcgtgac accggaagct
    16201 tcagcctctt tttctttagc cagctgttcg acttcgtgaa tgagggtgtc catgagttca
    16261 tccatgggca cctttcgcat caactttccc ttcttgaaga gaatgccttt gccttctccg
    16321 ccggcaattc cgatatccgc ttccttgcct tcgccaatgc cattgaccac gcaaccgagg
    16381 acagacacgt tcaacggggt cttgatatgt ccaagctttt tttccaactc gttcgccata
    16441 cgcaccacat cgatttcgac acggccgcag gtgggacagg cgatgacatt gatcccgcga
    16501 tgacgcaatt ccagcgactt caggatttcg aagccgacct tgacctcttc cactggatcg
    16561 gccgcaagcg agacgcgtag cgtgtcaccg atgccttgcg aaagcagcca cccgaggccg
    16621 atcgcggact tcaccgcacc ggtcatggcc gtcccggctt ccgtaatacc gatatgcagt
    16681 ggatagttgg attgagtaga gaacagatag taggcatcga tcgcgtggtg cacatcggaa
    16741 gccttgagcg acaccttcat gttggtgaag ccttcgtctt cgagcgcatg cacggcgttc
    16801 aaggccgatt cagacagggc ttcaggcgaa ggccagccat acttatctag caacggccgt
    16861 tcgagcgagc cgccgttgac gcccacgcgc aggggaatgc cccgctcgtt gacggccttg
    16921 atgacgtcct gcactttcca ccaggcccca atgttaccgg ggttgatacg gacgcaatcg
    16981 acgacttcgg ccgctttgag cgccaatcga tgatcaaagt gaatgtcggc aattaacggc
    17041 accgacatgg ccgccttgat cttcggcagc gcgttcgccg cttcgtcgtc gggaacggcg
    17101 acgcgaatga gctcacaccc ggcggtttcc aattgccgga tctgctccac ggtcgccttc
    17161 acatcgcgcg tatcggtcga gcacatcgac tggactgaaa tcggcgcatc accgccgatc
    17221 ttgaccgatc ccgcttgaat ctgccgcgtc tttttcctgg tgatatgcat gggtgtcagc
    17281 ggccttagct gccttgctca tcaccgtgcg gcaggctgcc gacgagtgaa gccaggcgag
    17341 cggcatcgtc cgccacgaca ccggcgccga acaactgctt gacgctgttg taaatgccgt
    17401 cggcggtgag accataccgc tcacgcaaca ggtcctgcgg cccttgctcg atataccaat
    17461 ccggcaaccc aaggatcttc gtgcgcagca tgactccggc ttcggacaat gtttccaaga
    17521 ctgcggaacc gaatccgccc atcttacaac cttcttccac cgtcaccaga caacggacgt
    17581 tcttcgccgt tttgaggatg agctcagtat ccagcggttt ggcgaagcgg gcgttgacga
    17641 cggcagcgga aatgccttcc tgcgccaacc gttcggcagc cttcatggcc ggccagaccg
    17701 tcacgccgat cgccacgatc gccacatccg ttccttcacg caataactct cccttgccga
    17761 tcggcagagt ggttggctca ggatccatcg gcacacccag gctgacgcca cgtgcgtagc
    17821 gcacggaagc agggccatca aaactcacac aggtcttgat catgtgctgc aattcgttct
    17881 cgtccttcgg tgcggccacg accatgttcg gcacatgccg gaggaacgcg aaatcaaagg
    17941 cgccatggtg cgtcgtgccg tcttcggcca cgagcccacc acggtcgatg cagaacgtca
    18001 ccggcaaatt ctgggtggcc acatcatgga cgacctgatc gtaggcccgc tgcaggaagg
    18061 tggaatacaa ggcaaccacc ggcttcatgc cctgcgccgc cataccggcg gcaaaggtca
    18121 cggcatgttg ctcagcaatc cccacgtcat agatgcgttc cgggaactct ttttcgaacg
    18181 cattgagccc cgtgccctca cacatggccg cagtgatcgc aaccacccgc ttgtcctgac
    18241 gcgcaacctt gatcaaggca tcgacggcca tgctggtgta actgggacgc acggctttct
    18301 ttgccggcac ccccgtttca cgcacaaacg gcgggcaggc atggaaccac acggggttat
    18361 ccatggcagc ctgatatccc aagccttttt tcgtgatcac gtgcagcaga accgggccct
    18421 tcattttcag cacattttcc aatgttggaa gcaggtgctc aaagttatgt ccgtcgatcg
    18481 gtccggcata ctggaagccc aactcctcga acaacaggcc gggaagaatc gcacccttgg
    18541 ccaactcttc ggcccgacga gcgatttttt gcatttcggc accgatgtgg ggaatcttcc
    18601 gcaggagctg gccggtttct tcacgcatcc tggcgtagaa ttcaccggtg aaggtccgat
    18661 tcaagtaggc ggaaatagct ccgacgttgc gggaaatcga catctgattg tcgttgagga
    18721 cgacaataaa atccttgttc gtcccgcccg cgtgatgcaa accttccagc gtcatgcccg
    18781 ccgtcatcgc cccgtcgccg acgacgcaga cgaccttatg tttttctccc cgctgctcgc
    18841 gggcttccac catgccaaag gcggccgaga caccggtacc ggcatgaccg gcattgaagg
    18901 tgtcgtaggc actttcttca cgcttgcaga agccactcaa tccgccgtat tgccgcaggg
    18961 tgtgaaactg ttcacgacgc ccggtgagga gtttgtgggt gtaggcctga ttgctggtgt
    19021 cccagacgat cttatctttc ggcgtgtcca agagatattg caatgcgacc gtcaactcca
    19081 cgacacccag attggaggcc agatgccccc cgacattcga aaccaccgcg aggatttgct
    19141 cacgaatctc ctggcatagt tccggaaact gttcagggga caggcgcttc aaatcagcgg
    19201 gactgtggat attttttagc agagacattg cacaatctcc ttgtcaaagc atgacaattg
    19261 gacgcaggta ttcatattcg tggaaaacaa tcgtcttgag aaggtcgtgg cgaagcagcg
    19321 aaagaagttc gtacaaatcg cctcatcctc acatatggca acgcctgtgt caagattccg
    19381 ctgtcctccg gaccatccga ccatcgcctt acgaccgaaa ccggtacgta agaaccgtag
    19441 aaagggtaaa atcggcggcg ctgccggtga tgaagtccag gttttcgact ccatagactt
    19501 gccacagcag cctcggcagt atccggtaac ttacccccag aaccgactcg gtcacccctt
    19561 tatccaaaac acgggttcct gtgccgtgaa acggggggga ataatagtcg aactgcgccg
    19621 tgatggaaaa attctccgtc cagaggtatt ccaccgcgac caacccgctg acgaccggtt
    19681 gcacatcaag tcccgcgatc cgtcctgtgg gaaagattcc gttcacgttg gcgtacagga
    19741 tccagccggt cgcgaaagtc ttgtcgaggg ccagaccgat gccggcatcc gggtggccgc
    19801 ttccgaatac ctgtccggtg tctcccgtgg gagctttgac cgccatacgg atcgacaatg
    19861 ccggctggct tccggtctct tgcaacaact ggtatttgcc ataaaatgag acatcgccta
    19921 agccggtggc attttccgat ccctggaaca tcgtacggcc accgttggac acgttgaagg
    19981 cgtacccggt atcccgcaaa gcctttcgtg gaggagacac tcccgtcgtg cctcgttcca
    20041 ccccgagaat cgcaccttcc ataaatccgc tgtaccgatg gtatgccggt atctccgccc
    20101 ccacctccaa tcgttcggtg aggccgtatc gaagaaacag accggcgcgc acggtctcaa
    20161 atttcatggt tacatcggcc tgctcctccc gatcccgggc gatcgtggcg gtattagccg
    20221 cttcgagacg gatatccaaa tcccccttgc gcaacacagc cgcccgctca ccaggcatgg
    20281 ccaacaccag ctggtccagt gcttgaaagt ttctgaccgg gaagggacca aacccctccg
    20341 cgccagccag aagcggcagg ccgcaggaaa gcccgatgag gcagaaaaaa tagacgagat
    20401 gccgcatgag taatacccgg ccgttcactc aggcgcttaa taagggaagt ccaacccggc
    20461 gaaaatcgct ccgccttcgc ggctatatcc gtaatccagg cgaccgacca cgttcggtcg
    20521 cacaatcgcc cgaaaaccga cacccggtgt catccggtaa tcctgaaagc tcacatcctt
    20581 aaaggaattg aacacctggc ccgtatccag aaacggggcg agttcaaaat ccgccgtcac
    20641 ccctgccaac ttcgtgcgca agatatgaat ccgctcttcg atactgaagg cgatcagatg
    20701 tttgtcgatg tagcgatcca tcccgaatcc gcggagattg ttttgtccgc ccagcgacga
    20761 ctgttcgaag aacggcactt gcgaaccgat cgtggcctgc aaatcggccc gtacgaccag
    20821 aatggcacgc ttggattcgc tggggaacaa ctttttcact tcgatttcgt accgtgaata
    20881 cacgggatga tccccatttt tcacattttg attcagctca gcataggcca tgacggacat
    20941 gccatcggtg ggcgtgacca ggctgtcgcg ggtatcgtaa taaaatgagg cgcgatgccc
    21001 gacaatgatg gattcgccct gaataccgtc gacagtcgga aattgctcca ctgaaaacgg
    21061 caggtctgtg gcgccccgct ggagttgtac ctggcgcacc cgctgcccca ccgaaacctg
    21121 cgttacctca ttcgcataga ggccgaggcg ccagtacgcg cgcgcttctc tcgccgtgta
    21181 attcgattca tccgcttgga ccgtcgattg gcccagcccg aagaaccggg acgtagcatt
    21241 tttaaagaag gttccaccga agttcagaaa atactgcccg ttaccgaacg ccgggtcgac
    21301 atagtcaaac aacaccttgc gctcgatttt ctctgtcagg gaggcgatca gcttcatctg
    21361 ccgccccccc ggatcgtact taaagaggtt gaacaccgcc cggctgccga cgatcgaatt
    21421 ctgaatcaac atcggcgcca tcaggtattt caattcgcca tccggatcgg agatcaggat
    21481 gggtgcgatc aaaccggcgt cgttcccgtc gtttttgctg gtggagaccg aaggaaccgg
    21541 aaaaatctgg gtatcggccc ctgcgggagc cggagagctc aggaacataa gtccggccag
    21601 cgccaggaga atacggatcg acgaaaaata atgtcgctgc atggcgctcg ccggaacggt
    21661 cgctttctat tgagactacg gggctttgat cttctcggat ttcttgcgga gctgatcaac
    21721 gaggtcggaa tacgaggagg tgtgaaggat cttcgtaaac tgcccgcggt aattgttcac
    21781 cagactgacg ccatcgacca ccacgtcata cacgtgccaa tcattacttt tgttcagcag
    21841 ccggtagtcc atggggattt cggttttgcc ggacaagacc ttggtgcgca cctccgcata
    21901 ctctttctcg gtgcgctcat tgaggtattg cacaccctcg ccggaatagg tctccacccg
    21961 atcggcgtag gtgttggtca gcaacgttcg gaacagatcg acaaactctt gcttctcctt
    22021 gtccgacagt tgattccact gcgcgcccaa tgatcgtcgt gacatttcgg agtagtcaaa
    22081 tcgcgccgcg acgacttttt ccaaacgctg ccgccggtct tcatgtttag ccggcgcctt
    22141 cagttcttta tcgttcaaga tcctcaggac ctcatccact gttccccgga tcgattccgt
    22201 ggccggaccg gccagggcga acgacagccc gcccacgaac agctgcagcg ccaacaggac
    22261 acccacgacc atccatcgcc ttacatgcat tccaacacct ctcctgtgag tggtgattcc
    22321 ctggtcccac cagcccaatg ttcgcattgc caccctctgc aaatgagccg gtgagattag
    22381 ttcaccttgc catgtacata ctggctaacc aattcctcca gatcaagccc tgactcagtg
    22441 tcacgaattt tgtcgccggg cttcaatgga tcgccgcccc cgcctacgga aagtgcgaga
    22501 tatttatccc cgatgatgcc acgggttttg atggaagcaa tcgtgtcgga gtacaacttg
    22561 acgccatcct gaatcgccag ggccaccaat gcccggtcac tattgaggcc aatggtgcga
    22621 acgcggccca cctcgacccc ggcaatctcc accgacgctc ccggcttcag tcctgaggcc
    22681 gacgtaaact ccgcttcgat cggataattg tgtccgccga tgacctccag ttttcccagc
    22741 ttgatggaaa gatatcccag gcaggcgatc ccgacgagca cgaaaatccc gaccatcaat
    22801 tccagttttg cacgttccat aacgtctcct atcagcgact ctcagccggc aaccctttgg
    22861 gcgcgaccgt gcccccgaca aaaataaact cgcgaatctc ttcatcggtc gtcttcacaa
    22921 actcgcttga tggtgccatc tcggcaatcc ggccgttctt cagcatcgcc acatagtcgg
    22981 aaatgccaaa aatttccgga atctcatgac tcaccatcac cgcggtgaaa cgaaactgct
    23041 gctgcatcgc cacgatgagg tcgtgaatgg ccttcgccat caacggatcg aggcccgtcg
    23101 tcggctcatc gaacagaatg atctccggct ccattaccaa cgcacgagcc agcccagccc
    23161 gtttgcgcat cccgccgctc agttccgcgg ggaatttatg ccccatgccc ttcaatccta
    23221 cctgctccag cttctcctca actcgcctgg tcacgacgtc acccttcagg cggagttttt
    23281 cccgcaacgg aaaggcgaca ttctcaaaca ccgtcatgga atcgaacagg gcggcgccct
    23341 ggaacagcat cgcgaatttc ttgcgcacct cgttgagagc ccgtcctttg aggcgggcaa
    23401 tgtccgtccc atcgatccag acttcgccgc gatccggctg catgaggccg atgatgtgtt
    23461 tcaacaacac actcttgcct tcgccgctcc gcccaatgat cgtcgtcaat ttccccgtgg
    23521 gaatggtgag gtcgacgccg cgcagcacgg cctgtttccc caaggtcttt tctaccccga
    23581 ccagtttaat catctgaatc cagcaattta tgtcatcagc gttcgggaat ccgttcacat
    23641 ccaaacgctg aatgcggacg gccgatgact gacagctcta tagcaacacc gaggtcagaa
    23701 agtaatccca caccaggatc aacacggcag acagcaccac cgcttcggtc gtggccgttc
    23761 ccaaaccttc ggcactgtgt ttcgtgtgaa agcccttgta acagcagacc caactgatca
    23821 acaggccgaa gctgatggat ttgagaatgc cgccatagac atccttccat tccacagccg
    23881 actcgattga actccaatag gcgccttcat tgccgttcag cagttgcacg ccgacgacgt
    23941 atccaccgta aatgccgacg acgtcgaaca gtgcgaccaa taacggcaca gcaatcaaac
    24001 tggccaccag cttgggacca atcagatact gcagggggtt gatggccatc gtatccagag
    24061 catcgatctg ctcggtaatc cgcatgatgc cgatttcagc cgtcatagcc gatcctgcgc
    24121 gggccgtcac catcagcgcg gcaagcaccg gccccaactc acgaatgata ctgagcgcaa
    24181 cggcggatcc cagcaccgcc tcggagccga atttccgcag cgtgtagtaa ccctgcagcg
    24241 ccaaaaccat gccggtaaag acggcggtga ggaccaccac gaaggtggat ttatagccga
    24301 tgaaattgag ttgtttgacg atttgataga acctgaacgg cggacgggtc agccaggcaa
    24361 aggaggagag aacgaacagc atcattcgcc ccatctgctc cgtcagatcg atggtaaacc
    24421 gtccgatgcg agcaatacct tccacgcctc accctcctct tctctcacgg gccggacgac
    24481 cgccgcgcat aactcctgat cagccgatgc cttcacccag agcacgatcg gcacaggcac
    24541 gaaacaacgc cgtcaactgt gcgccggcaa tctgactctg cactcggaga cgattgagcc
    24601 cgatcaggct cgtcggatgt gccaggcacg cgacgacacc cttcgcccat ccagatggcc
    24661 tgagaaacag gttgaagtca agcggcagac tctcatcctc gagatcggag accgtgcgga
    24721 cgatcgcaaa agggattcga tgctccgagg ccaccctccc taaggccgcg ctctccatgt
    24781 cgagaccgat gcctccggct cgtccggcga tcgcgcgttt ttcttccgcc aggcagagta
    24841 cacgcggcac cgtgacaaac cctccgcttc gcatcggcaa gccttgcaca tggaccgcct
    24901 gcctcaccca ctcgaccatc gccggcgcgc acggcacaga aggccccgcc ttcccgccgt
    24961 tttccaggat cacgtccgta ccaatcaaca cgtccccgat gccggccggt cccagagcac
    25021 aggcaaatcc cgtcgaccag atggccgaca gcgcctgttc cgcgagtatc cgtttggccg
    25081 tggccatcgc ccgctccgga cccactccca tgggaatgat gcaccattcg acgaacggct
    25141 ggcgtaccac cacacaacga attccaccga cggcccggac ttcgctcgca ggaaaggcgt
    25201 gccgcacggc ggccaattcc caccgggtgg caacaaagac accgatcgtg gtcacagcgg
    25261 ctcccgataa ctgatggcga ggggaaatgg actcgatgag catccacggt cagcgaggcg
    25321 accggaactg tcctgattga taggccagct gtcgcaattc atccgccctc gtcgttccac
    25381 gagtcttcag attgcgatac atggccagcg cccagagagg gaagtattgg caataccaat
    25441 gataacggag gtagaacacg cgagggaaac cggttccggt atggcgcacc tcttcccatg
    25501 acccgtcctt ccgctgattt cgaatgaggt agtgaatacc tcgggcaagg ctgaatgagt
    25561 ctgtgacgcc gcctgccatc agggccaaga gcgcccaggc agtttgcgac ggggtactat
    25621 cgccacgccc gctctccgcc acatccgcat aggagagaca ggactcgccc catccaccgt
    25681 cgggattttg cttcgactcg acccagctga ccgcacgacg gatataggga gaagagaggt
    25741 cctcaccgat ggcccgcagc ccggagagca ccgaccaggt cccgtaaatg taattgacgc
    25801 cccaacgacc ataccaacta ccgtcctgct cttgatcgtt cttcacaaag gtcaacgccg
    25861 aggccacgga cggatgcgtc cagtcatacc ccagggtggc cagcatttcg agacagcgcc
    25921 cggccaggtc ggccgtactg ggatccaaca atgcgcgatg gtcggcaaaa ggaatgtagt
    25981 tgaagacgat tcgattattg tcgacgtcgt acgcgcccca cccgccgtcg gatccctgca
    26041 tcgcagtcac ccaccggcac cctcggcgaa tcgccagccg ctgctgttcc tcatcaggca
    26101 aacgcacttt agcaagcgcc atcagcacga cagccgaatc atccacgtcg gggaagagct
    26161 cgttctcaaa ctgaaagtac cagccgcccg gttccgcgcc gggggaggaa ataatccagt
    26221 cgcccacggt ctttgtttgt ttggataaca accaggtggc ggctttctgc aacgccggat
    26281 gatcctgcga cataccggcc tcggacagcg cattgatgag caaggcggtg tcccaaatcg
    26341 gagaatggca gggttgcaga tgcagcgcat cgacacattg tccgttgtcc atcaccgtat
    26401 cgtgaatttc gagttcttcg atctcccgca tcgctttgac gacgagggga tcatcattct
    26461 tatagccgag gcaattcaga gccatcaccg aattggccat ggcgggataa atcgcgccga
    26521 ggccaccgct ccccttcata tgatcgagca tccagttctc ggcgcccttg agcgccttct
    26581 ggcgaaccca ttcgaccggc gagcgatcat agaccttgag cagcgcgtcg agattgatga
    26641 aaaagtttct ggccgtaaac caggtccggt ccttcttgaa tggagggacc gtgctgtaat
    26701 caatctccgc cgggggctgc gtgtacagct cgtcgatgcc ctgttcgggt ggcacataac
    26761 actggggccg tttggcaaaa atgatcaaga gcggaatgag gaccgcgcgc gaccaataag
    26821 aaatggcgta gatgctgaaa tagaaccgct tcggcaacag catgatttcc ggcggcatgc
    26881 tgggcacgcc gcgccaatca tactgaccga ataaagcgag ggcgattttt gtgaacacat
    26941 tggccgcgac gaccccgccc ttatcgagga tgcaggttcg cgcctgggcc atgaacggtt
    27001 catcggccga gacgccggaa agcttcaagg caaagtacgc tttcacggaa gcgctgatat
    27061 ccgccggccc cccgtgataa ataggccagc ctccacacgg aagttgggcg gacttcaaat
    27121 agcggatgac tttgcgctca cgctccgggt cgacgcagtt catgaaccgc cgcagcatca
    27181 gatattcgga gctcagggtc gtatctgctt ccagctcagc tacccaaaaa ccttcggtag
    27241 tatcctgcct ggccagaaac cacgcctgac tcttccggat ggcttcttcc agtgcttcga
    27301 gttgaccggt ggtgtgtgaa ggcgctcgct ttgcaccgtc gctggctgga gcatcgggct
    27361 tactggagac gaggcgcagc acgggagcag aggcaaaatc gcgcgccgct ctgattcggc
    27421 tcggcaccga gaccagcaga ctatcagaca gacgactgaa gagattcttg agaagtcgca
    27481 tagcgtgttg ccgtgaatca gacagagacg gcgggatcga tagtcgaaac ggttccatca
    27541 atcaagcagt gatcacatga aaccgcacag taccatgtat aaccattagc aggactgtat
    27601 aacaaactgc ttcagaccag tcaagggaag tagccctcga agtgcgcgcc aagtgcgcgt
    27661 ttgagcagaa gactcagcgc gctgcttccc cgacaccgtc ctcattaggc cggctgcaaa
    27721 tcgccgagct tgacggagat caaggtcgac acgccgggct cttccattgt gacgccgaag
    27781 agcgaatccg ccatcgccat cgtccgcttg ttgtgcgtga tgaccatgaa ctgggcagtc
    27841 gaggacaggc tgcgcaagac gcttgtgaac cgcccgatgt tctcttcgtc cagcggcgca
    27901 tcgatttcgt ccaggatgca gaacggcgtc ggacggatca agaagctggc aatcaacagc
    27961 gccatcgccg tcaaagtctt ttctccgccg gacaacatgg taatactctt caatcgtttc
    28021 cccggcggtt gcgccacgat ttccacaccc ggctcgcgcg cgccgttatc ctccacccct
    28081 tcttccaacg gctcttcgac caactgcagc tctgcgcggc caccgggaaa gaattgtgcg
    28141 aacacatccc ggaatttctg ctgcaattcg ttgaaggtct ccacgaacat gtctttggtg
    28201 gtccggttga tcttctgaat aatttccttg agcgaggcaa tagaggtaga cagatcctgc
    28261 tcttgtgtcg tcaaaaactg ataccgttct tccaattcac gatgttcgtc gatcgccgcg
    28321 agattgatcg cgcccatgcg gtccaaccgt tcccggattt tctgaatctg ttctcgcaac
    28381 tgtggcgtct cgagcagaga gaccggtgca gctggctctt ctccttcagt tacgggctgt
    28441 tcctcagttg ataccaccag cagagtagcc ggatcgattt gataggtgcc ggccagcgtg
    28501 ctctcgatgg tcgagagatg cgccttcact tcggccttgc gcacttcagc cgtcatgcgg
    28561 cgatcatgca aggccgacaa actctgccgc acggtggaga gggtttcttc gacggtatgc
    28621 aatccgctca tttcttcagc ctgacgttcc tgcgccgcga tgagttccga tttgatgcca
    28681 tccacctcgg caccgagttc acggcagagc gcctcctgcc tggtctgctc ctcgcggctg
    28741 ctctccgtag cctgtaccag tccggtcact tgctcctcga gctccgccgc acgtcgctgc
    28801 gcagcatcca gccgttgcgt gaaccgcgca atatcgctgt gagcatgctc gcgacgtcca
    28861 cgcatgcttt cgagcgacaa ccgcacttcg gtcagccgtt gctggatcgc cagcccttgg
    28921 ctttcgatga cgaccagtcg ttcacggatc tgaagcaacc cgttttcctg gccgagcttc
    28981 tctgccaccc attgctcaag ttgagcctgg ctggattcca actgctcctg caaccgcgcc
    29041 tgttcagcca atccccgctg cagctcttcg gccaacatgt ccatccgccg gtgcaactct
    29101 ccacgctggc gctccacgtt tgtatcgtct ttctgaaggg ataactccag catctcggcc
    29161 tcgcggatgg ccctgcccag ctgctgttct tcctcacgcc ccagacccaa tgcggaggct
    29221 gcttcctcac gtgcgacacg agcgagttcc agcgcttgca ccgcttccat gcggcgagcc
    29281 tccaactcca gcacttcccg ccggcgctgc aggagtcccg caccggcact ggtcccaccc
    29341 ccggtcatga cgccggctgc atccaacgtc tcgccggaca aggtcacata ggttgggcct
    29401 gcaggtgccg gccactgatg ctgttcccac aattgcagtg ccacagcgag ggattcgaca
    29461 atcacgatgc catcaaacaa ataactcaag gtggaagcgg atgcgccttc tgcgcggatg
    29521 acatcgatgg cacgccccac gactcccggc tgcccattga gtgcaggcca ccaggaagct
    29581 gaagaatccg ctgctgcctg gtcggtcgcc caacggatct gctgagggat aaatgcgcca
    29641 cgcccaagtt ccttaccttt caaaaactcc accgctcggc tggcggctga gggctgatcg
    29701 accaaccagc cccgtacccg ctcacccaga atcgtctcca ccgcacgatc cagcccggac
    29761 ggaatgacga gccactctgc aagcgcctcg cgcacaccgt cgcaggtttt cagcgcggtg
    29821 ccttcttcct ctccctcacg gccgtagccc atttcctccc gcaccacacc ctgcaacgcg
    29881 cccaggcgcg attccactgc agccacatct tcggaaaatc ggacgatatc gcgatccaag
    29941 cccgtgatct gcccgccgag cccttcaata cgctcgatcg cagcctgccg ttccgccatg
    30001 agtccctgga tccgctgact ggcctcttcc cgctgcgccg ccagaagttc ccgctgctca
    30061 tccaatccag ccacttgcgc gaccatctgc tcctgctcac gcgagacccg ttccgcccgg
    30121 gctgacgttt cctgctggcg cgcggtcacc tgcgcgagac tttgctccgt gttcgctacg
    30181 agcacggcga ggttcagcac atccttccgg gcgcgctcct cttcgctgac agcggcggcc
    30241 cgatgccccg ccagggcttt cgcttcagca tccgcctgct gaaactgcgc ctcttgctcg
    30301 acaatgtcgc cttccagttg cagcaaggtc gctcgcaggt cttcgatttc aacctgcgcc
    30361 tgctcctgat cccccgccag tcgctccatt tcctgcgaag cctgcacgcg ttgccgctcg
    30421 aacaattccg tgcggttccg ctccacctcc gcggccgtca gtgcctgcga ttgccgctgc
    30481 tcggtcccgg ccaaggtatc ccgcacgcgc gagatggcct cggccgtgtc gttcatccgg
    30541 agccggatgg cttcctgctc cgaatccaga cgagcctgct cggcgacctg ctcggcctcg
    30601 agtgcttcca cctcccgcgc ttcgcggtcg acggattcca ggtcggcatg catggtgcga
    30661 tagtcacggg acaacaactc gatctcgagc cccttggctt cctgctgtag cgtttggtac
    30721 gtccgggcct ggcgagcctg gcgttccagt gaattcagct gcttcttcac ttccgccaca
    30781 atatcgcgca cccgcaggag attttgttgc gtggattcca gcttacgaag cgcttcagcc
    30841 ttctgttttt tgtaccggac gatgcccgcg gtttcttcga tcaattcccg acggtcctgc
    30901 ggtgacgcct gcagaatctg ctcaatgcgt ccctgttcga tgacagtatg gccctttgac
    30961 cccgctctgg tttcaatcag cacattgcga atatctttga gcctgcaggg cgtcttgttg
    31021 atgagatact cgctgtcgcc attgcgatag agacggcgcg taatcatcag ttcctggtat
    31081 tcgctcaatt gactcggcaa tcctgaaatc gcatccaatc gcaactcgcc cagtcccccg
    31141 atcaccagcg agacttccac caacccgagt ggtttccgca cttccgtccc gttgaagatg
    31201 acgtcttcca tcttctcgct ccgaagcgtt ttggtgcttt gttcgcccaa cacccagagg
    31261 atggaatcca ccacattact tttgccgctg ccgttcggac ctacgatcgc cgtaatgcct
    31321 ttgggaaatt gaattttcgc ttccgcgaat gacttgaagc cgagcatttc caaggacttc
    31381 agatacaccg gcgacctcca gagcaagaag gttgagaagg gacgcgttgt gaggaagaaa
    31441 acgcaaaacc ttctatcaca gcgatagaat caatgcaaag gaaaagccct tgatatgggg
    31501 gaataaagag gccactcccc caatatatgg aggaatggcc tgatctacct gtaggacaat
    31561 atgaggacgg ccgagcagtt agttcgcaga ggcggccgtg cgtgatgcag attcaatggt
    31621 gacgagttct ttcggcagga ggaattccac atcctcttca atgacggtca attcttccac
    31681 ttcttcagaa gaacccagac gcttgagata ggccaccact tccgtcacca acacttctgg
    31741 cgcagaggcc cctgccgaca ggcccacact cttggcatcc ttcaaccagt ccggattgat
    31801 atccgacgcg gcatcgatga gataggaggg aatgccgcaa tgctcgccca attcgcgcaa
    31861 ccggttcgaa ttggagctgt tcggcgaccc gatgacaagg atgacatcga cgagcttaga
    31921 caaggccttc accgcattct gacggttctg cgtcgcgtaa caaatatctt cctgatgcgg
    31981 gcctttgata ttggggaatc gccgatgtaa ggcctcgacg atatctcgac actcatccac
    32041 actgagtgtc gtctgggtga cataggacag gttctgcgtc ttttccacgt ggagcgtttc
    32101 gacatcctgc acggaggata cgagatggaa cttatccgga atctgcccca acgtcccaat
    32161 cacctcgggg tgcccagcat ggccgatcag aatcaattca tagccttgcg tgtaatcacg
    32221 gttgacttcg ttatgcacct tgatcaccaa gggacaggtg gcgtcgatga catgcaggcg
    32281 gcggctctgc gcctcttccc ataccgactt cgccacaccg tgggcactga agatcaccac
    32341 tgatccttcc ggcacctcat ttaactcctc cacgaatacg gcacccttgt gccggagcga
    32401 gttcaccaca tgccggctat gaacaatctc atggcgcaca tagatcggcg cgccgtattt
    32461 tttcagggac agatcgacaa tatcgatcgc gcggtcgaca cccgcgcaga atccgcgagg
    32521 attggctaag tatatcttca tctgctgatt ccctctcgcg atcatgaagg gctgcccggc
    32581 ttgccggggt atacacatcg tctcactaag cggtcacctt acgtcagacc cgcccttgac
    32641 gtcaaccgcg tcatccgcat cttgccagcc ccaaaagcag ttgaggccgt tgccccattg
    32701 tgcaacaatc cggccatgtc atcaccttcc agcacgcaca aaaaaattct gatcgttgaa
    32761 gatgagaaag atattctcca gctagtcaaa ctctacctcg agaaggaggg attccggacg
    32821 gtgtcagcca tgaccggcac ggaaggcctt cggcaggtga aggccgaaca tcccgatctg
    32881 attattctcg atctcatgtt gcccgaactg gacgggctgg aggtgtgcaa acggatccgt
    32941 ctgaaccagg agaccgccct gctccccatc ctcatgctga ccgccaaggc cgaagagtcc
    33001 gatacagtga tcgggcttga gctgggtgct gatgattacg tgaccaaacc tttcagtccc
    33061 aaagctctgg tggcgcgggt caaggcatta ctccggcgac tggatcgcaa cgccaacagt
    33121 cctcagaccc aatatcatta tggccccttg accgtagatc tgtcgcggca tgaagtccgg
    33181 ctggagggag aagaggtgac gctcaccgca aaagagttcg gcctcttgga gcatctgctc
    33241 cgcaatgtcg ggcgggtgct cacgcgcgac gtcctgctca gcgcggtgtg gggatatgat
    33301 tattatggga ccactcgcac ggtggatgtt cacgtgcgcc ggctcaaaca gaaactcccg
    33361 cttctggatg acgccatcat ctcgatcaaa tcactgggct acaaactgcg cgagtctgat
    33421 attccggcat gaacctcagc ctacggtgga aagtcgccct gggcacactc gccgccgtcc
    33481 ttgtcggcct cctggtcgcg ggctggctcg tgatccgttc ggtggaacag accgaactca
    33541 gccgcatggc ggaaatgctg gagacgcgca gcagcttggc cgccatggcg ctcaggccgt
    33601 tgtttgatca aaccgggcaa gccgttccca gccccctgct tcacaccacc atccgcgagc
    33661 tcagccagca ggcccgtctt cgcatcaccg tcatcaaaca ggacgggacc gtgctctcgg
    33721 acagcgcggt ccccgccgaa ggactcactc acatcgacaa tcatcttgcc cgcccggaag
    33781 tggcccaagc gctggcatcc ggccggggca tggatattcg agccagccag actaccggag
    33841 agcgcacgta ctatgtcgcc cggctccttt ccgaaccggc gcgcatacaa ccaggcgtcc
    33901 cagtgatccg cctcggcctg ccattaacct ccatcgatga acgggtacgg cacattcagc
    33961 aggacttgct gacagcgttc ggggccgcct ttctcctggc gatggtgctc agcctctggg
    34021 tgtcacgcaa tctcacaaag ccgctctcgg agatggctgc tgccgctcgc caactcgccg
    34081 cggggacgcc cgggatccgc ctcaccgtct cgtcatcaga tgaagtcggt ttgctggcgc
    34141 gaacactcaa ccagatgacc gaccagctgg agacgaagat caaggaagtc tccgacgacc
    34201 gcgcgcaact cctggccatg ctgattgcga tggtcgaggg cgtcatggtg ctggactatc
    34261 gaggcacggt tgttcaggtg aatccggcgt tggagcggat gttcgcatta gagctgacgg
    34321 aatcacgcgg ccgtcattat gccgaactga tccggcacga agggctgacc gcgctggtct
    34381 cggccgtgtt gcagacgcga tccggtcagg gcggtgaaat tacgctctcc cccagcggca
    34441 gctgtttgcg ggtggaagcg tcgatcgccg gcggcaatcg tgagcaggaa gcctgtgccg
    34501 tctttgtctt ccatgacatt accgaactgc gtcgattgga gaaaatccgc aaagattttg
    34561 tcgccaacgt gtcgcacgag ctccgtaccc cgctcacctc gatcaaggga tacgtcgagg
    34621 ccctgctgga cggcggcaag gacgaccctt cgacagcagc agcgttttta gaaatcatca
    34681 tgcggcaaag caatcgcttg aacctcatcc tcgacgatct actgcaattg tcgcagatcg
    34741 aatccggcca ggtactgttt cgcagggagc cggtggaatt gcgggccttg ctggaacgga
    34801 cggttgcggt gatcaagccg ttggcggaca agaaacatca tacgatcgaa ttgtcgcttc
    34861 cggacgagta tgtcgtggtg gaaggggacg aggaacgcct cgttcaggtc tttattaacc
    34921 tgctggaaaa tgcggtcaaa tacacgccgg atcagggccg gatctctatg gcaatccgcc
    34981 aggcgacgca aagccggacg gcgtcaccgc gtccgatgat tgagattgtc gtggcagact
    35041 cgggaatcgg cattcccgaa gcggaccggc cgagggtctt cgaacgattc tacagggtcg
    35101 ataaggcccg ctcgcgcgaa ttgggtggaa cgggtttagg actcgccatt gtcaaacaca
    35161 tcgtcgaagc ccatagcggg catgtctggg tggagggaaa tgcgcccagg ggcagccggt
    35221 ttgtggttca cctgcccgtt gcgcaggagt cgtctacgac aatttcgaca gcagcaggta
    35281 acatatagtc gacaggagcg cagctccggg gagggtgaag agccaggccg acagaatgcg
    35341 acgggtcaca ccccagcgca ccgccgagac ccgcttgacc gctccgactc ccatcaccgt
    35401 cgaagtaatg gtgtgggttg tactgaccgg aagtccgata tgcgccgtgg ccatcaacac
    35461 gatcgcagct ccggtttccg ccgcaaagcc atggaccggt tccagcttca cgatccgcat
    35521 ccccaacgtt cgcacgattc gccatccgcc caccgccgtg ccaagcccca ttgcaagagc
    35581 gcaggatccg atgacccagg tcggcacttc cgccttggga agggcccctg ctgagacgag
    35641 cgccagcgtg atgatgccca tggctttctg tgcatcattc gccccatgac tgaaggccat
    35701 aaaacttgcg gagacgagtt ggagccgcga aaacaatctc aaggccaagg ctcgtggcac
    35761 acggaagaag agccagctca atacgatcat caacaccagc ccgatcgcaa acccgaaaaa
    35821 cggcgacagg atcatcgcct ccagtaccga gcgtaatcct gaaaatttga cggccgccat
    35881 cccgccatgg gtcagcgcgg cgcctaccaa cccgccgatg agggcatggg aggagctggt
    35941 cgggagaccc agcatgaggg tcatgaaatt ccacacgatg gcaccggcca gcgcagaggc
    36001 gacaacggtc tgggtcaccg attgaggatc gacaattccc gtgccgacca tcttggccac
    36061 ggcagtggac atgaacgctc cggccacgtt gagcgcaccg gcgacaagga ccgccgtaga
    36121 aggactgacc acgcgcgtcg agaccaccgt cgcgatagca ttggcgctgt catgccaccc
    36181 gttggaaaag tcaaacagca gcgccagaat cactacgact aacaacaatc cgctcagttc
    36241 agccatgaat gccgcgtcct atccctcgtg tattggtcag gatgagtatc gaagaatgtg
    36301 cgagggttgc tcacgtgtgc ttcaaggcga tgcgctcgag aatgttcgcg acatcttcgc
    36361 accgatcggt gcccgcctcg aaattttcgt agatttcttt ccacttgatg acggcgatcg
    36421 gatccgtctc cttctcgaat aaagacgaga tggcatcgcg cgaaatacga tcggcttcgt
    36481 tttccaggct attcacccgc acgctgcact ccgtgaccgc ctgatgggac ttgccgagta
    36541 agtccaccgt ggctccaacc tcaaccgccg cctgatacag gacattggcc aatctgatcg
    36601 ccgcctccgt tggagcgacg accttataca agacgaaccg atcggcgatt gcctcgacca
    36661 catccagaat gtcgtccagc gcactggcca aatcatggat gtcttcccga tcaatgggag
    36721 taatgaaggt ctggttcagt ttccgggcga tgtcatgagt gatgccgtcc ccgacatgtt
    36781 cgacatcctt aatccgtttg gctttctcaa ccggattccg aaagtcttcc gtcatgtcct
    36841 tcaaaagacg gctgccttcg atcatgttat gcgccgcatt tttgaacaaa tcgaaaaacg
    36901 cttcttcccg gggtatgaga ccgaacataa cgtcctttct agccgcgagg ggttaccaac
    36961 gcaccagtcc tgtggcccag gttaagcccc cgccaaatgc tgccaatact acgacatctc
    37021 cagctgcaat cctctgctct cgaaccgcac agtctaacgc gatgggaagg gacgcggacg
    37081 aagtattgcc atagcgttca atgaccgaat agagcacctc tcggggtagc ccgagacggc
    37141 gacgaagttg ctccaaaatc ctggcgttgg cttggtgagc aatgaccctc ctcacatcaa
    37201 cggtcgagag cccaaattcc ttcaacagtt cgagcatcgc ctgttccaaa tgacggatcg
    37261 cggcgcgaaa cacggcactc ccccgcatcc gcaggaggtg atctttgcca cggacggtct
    37321 ccatcccgct cggcttccgc gaccctccag caggaatggt gatgaggcca tgccctgccc
    37381 cctctgcata caggcgcacg ccaagaatgc ccgcggcctg cgggcgaatg gattcttcct
    37441 gcaccagcac cacggcaccc gccccatcgc cgaacaagac ggctgtttct ctgtccgccg
    37501 gatccagcga gcgggacttc acttcggccg caacgaccaa gcaggatcgg atgtgaccgc
    37561 tccggatcat cgcgtcggcc atcgacaacc cgtagagaaa ccccgaacac gaagccgata
    37621 ggtcgaacgc catgaccgac tccgccccca gggcacgctg cacgtggcag gcggtggaag
    37681 gaaaggcgct gtccggtgag gtggtcgaaa cgagaatcgc gctcagggat tgcggcggta
    37741 ttccggcagc ctcgcaggcc cgccgtcctg cctcgaccgc cagatccgat gatgcctgcc
    37801 ccggttcggc ccaatgtctc gtgcgaatac ccgtcagggc cgtgatctga tccgaattca
    37861 gcccaagcgg cttcccgacc tcctcgttat ggaccactcg agggggcaca aaggaaccgg
    37921 tcccgacaat ccgactgcac ttcacggaat tcctactgga gccttctggg atttggtttt
    37981 acaacgcgtc gcaccccatg cagaggtgcg gcgcgattat aggacccggc ttcaggaggg
    38041 tcaactccgg acattgattt cgttgctttt catttcacga tctgctagaa aaattgtgta
    38101 gttacgttgt ctctaccatc aatgcttgct gtgcgaagga gtctggatgc atcggcattc
    38161 gagggattgg tgtgtgcgca tcgccgtcat ggttggactg ctgtgttttt cggccgggtg
    38221 ttccagcaaa cccaaaacca cggcccaagg caaacccgtg agcggaaccg atgagcaaat
    38281 ctttcttggc gacaccatcg agaaaaatta cgatcccaac gtcatcatga agcgcggcga
    38341 ggcctttttc gacaaggaag agttcgccga agccatcgtt gaataccagc atttcctgga
    38401 attgcatcgt agccaccagc ttgccgtcta tgcccaattt cgcttagcgg aaagccatct
    38461 tcggatggga aaatcgattg accgcgatcc cgatcccatt cagaaagcca ttaccgcatt
    38521 tgaaaaattg cggaaggact accccaacag caaatacgag gcccaggcgc tccagcgtct
    38581 cgctgactgc catgattggc tcgcgaagac gcatctgttt gttgggcaat tttactatcg
    38641 tcgctcgtcg tatctagctg cggcccatcg gttcgacatg atcatgaagg attatccgga
    38701 caagacggta gccccggaag ccttatacta tctggcgttg acgtaccagg agctcggcgc
    38761 gaacgattgg gcgatggaaa aactccagct cttagccgaa aaatatccga atagtgatgt
    38821 ggcgggaagc ggccgtcgat tactggccaa actagagaag aagtcgccgc cggcggccat
    38881 cgtggccagc aaagaaacgc cggctccaca gagcccacca tctaacacgc tcctcgcggc
    38941 ctccgtccca tcgacctttg ctgcgcccgc cgtccccgag ctcaaacggg cgccgagtgc
    39001 tgccttgtcc cagcccttcg tctcatgccg gctcggagcc tggtgctgac ctagcgctta
    39061 cctggctcac taccctatcg cagatgcaat gcttcgagga ggaggtcgtt gactgacgcc
    39121 ttcaggcgct tcttcgcgtt ggggaggaat tgctgagccg tcgtacgccc ctgcagcact
    39181 tcgaaataat cctgaacgac atcctgataa cgccccaaaa gcttgaaggc caggcgtggg
    39241 aacgcataga taacctgagc cattctggcc gtcacgcgaa agtccggaag gatttcccgc
    39301 cgcagagcag ccgcgtaggc agacaccgcc atgtggtggt catctcgatg ggccagtatc
    39361 gcatgagccg ccagttgtcc tgaacggatc gcgtagtaga tcccctcgcc aaacaacggg
    39421 tcgaccagat gggccgcgtc accgacgagt gccgcgcggc ctgcgacaaa acgggactct
    39481 tcgtcctgct cccgcttttc agattctgta tacacaggaa tcggatgccc tgccggacga
    39541 ggcaccgccc ccccgatcac gtgttgctca tccgtgacaa agcggtcaaa ggtggcgcgc
    39601 aggctcttcg atttccggcg aaactctccg actcctacag aaaggcatgc tctcttcggg
    39661 aatatccatc catacccctg acgtgccgcg cccacatcta cgagaatcgt tgacgcagaa
    39721 tgaccgtgtc tgccattgcc aaggggaact tcgctttcca atgcgggagc ccggtactga
    39781 gatcgttgtg ggaaaaaccg cctggccacg acactattcg cgccatctgc gccgacgacg
    39841 atctgcgccc gatagcgtcc ctgtgctgtg acaacttcca cgccatgttg atcctgcatg
    39901 agttccacca cgccgtggcc ggtccggata tgcgcgccgg cctgaatcgc ttcctgcacg
    39961 agatagtgat cgaaccggtc ccgcatgacc atgtaggcaa tcggccgcgc tgattcgatc
    40021 agcagagggt cctggccccg atagacaaac tgcacaccgg tcacagactg ctcgaccacg
    40081 gagtgaaaac cgggttccag cgcgtgatca atacgcgccg acagaccgcc gccacacact
    40141 ttgtaccggg ggtgggcatc cttatctaaa ccaagtaccg tcaaaccgcc tcgactaaga
    40201 gccgcagcag ccgtcgctcc ggccggcccc atcccgacaa tgattacatc gtacgttgtg
    40261 ggtccgtatc catcagccat ggttacacac cggttgtgaa agggcttgcc gcgcaagtct
    40321 tcggcgacca tcgtgagaaa ttgttgctgc gtgggagagg tcaggaagag ccggagaggg
    40381 aagcactcat gaggcctgat tattgaccaa gataccagcc aatcccgtat cgctcgagaa
    40441 agctcgtaat catggtcaac gaatttccat agatcatcac gcccacgaca atgagcaaca
    40501 ccccgctcac cgtggagact ccccaaagat aggagcgcac ttccttgaag tagctcagga
    40561 accgatcgac ccctagcgcc gtgaggaaca acggcaaggc taatcccagg gaatagaacg
    40621 tgagtaacac gactccactg aacatcgatt cggtcgtgct cgcatacaga aggattgttc
    40681 cgagcacagg cccgacgcac ggcgtccaac ccgccgcaaa tgcaatgccg atcaagaatg
    40741 atccgagaaa gcctgccgga cgattccgga attgataccg gtgttccatt ttcaggaagt
    40801 tcaaattcag aatgcccagg agataaagcc caaacacaat gacgacaatg cccccgaaac
    40861 gccgcaaatg ctcctgatag gtaatcaaca cctggcccaa cagactcgcc gaagctccga
    40921 acgccacaaa taccgttgag aaccctgcga taaacagcaa ggagttcaaa atgatggcct
    40981 tgcgaaaacg gcttcgctct gaaacgtcgg ttagttgctc gatcgaaagg cccgtgatgt
    41041 aggaaatgta ggacgggacc agcggcagga cgcagggaga gacaaatgag agaaggccgg
    41101 ctgagaaagc ggccaccaag gaaatcgatt gcactgagtc ggtcatacta ggacccttgc
    41161 aaaagcgctt cgaccaaccg tttccccgct ggcgcgctcc agtctcgagc gccgggaatt
    41221 cgattccgaa tgataccttt gcgatcgatc aaaaatgtca tcggcagctg tcgggcgcca
    41281 tactgaagtc cgattctgaa atcggcgtcg tgaaggacgg gaaatgtaaa gccgacttcc
    41341 tgctgaaagg ggcgggtgac ggccgtgcct tgggcatcgg ttgaaaccgc cagaatctcg
    41401 aactccttcc ggctaaacga tcgataaagc cgttccatcg ccggcatttc gattttgcag
    41461 ggaccacacc acgtggccca aaaattcaga agcacgacct tgccgcgaaa ctggtccaaa
    41521 gtgacattga acccctcgga atccttcagt agaaagttcg gagcctcttc atcaacctgc
    41581 ggcgcagggc tcgccaccag aattggagct ggggctgggc tcaatagggg cgaggcccat
    41641 ggagcgtcgg caatcatcac tccgcctatc atcaatccgc cgacaattat ggagagcatc
    41701 atccaacggt tcatcatccg cctatagcgc tgggactgct tcaacagtgg ctgcacttgc
    41761 cgccttttca gcaggcttga gcaattgcgt caggactttg agattatcta atcgcgtcca
    41821 atcccgagga ccaatcactt tttcgcgcaa cacaccttgc tgatcgatga tataggtttc
    41881 aggaactccc atcaatttgt agcgtttatc cgtttgtccc caggagtcga ccaggacggg
    41941 aaaggtcaga ttcaggcctt tgacaaaggg cgggatctct ttcttagtcg tgacccggtc
    42001 gatgctgact gccagaatca cgagtccgtc cttttcaaaa ttcttgtaca gcacttccat
    42061 ggaaggcatt tcttcccggc agggtttgca ccaggtggcc caaaaattca aaaagacgac
    42121 tttcccccga taatcagaca agcgtaaggg cttgtcgttc aagtcaggta gctgaaagtc
    42181 gggggcctct tttccgacga tcaacggctc atactttgag ctctgcagcc agacgacgag
    42241 aaacatcacg ccaagaatgg ctgcggcagc cagcaccacg accgttctgg aaggaccact
    42301 tcgtgtcgta acgccgcttg aactttgatt cagaatatca ctcatggtct acgcgtacgc
    42361 atgaagacca gagagcagaa aattcactcc ccagaaacag aagatcacca tcagaaaccc
    42421 gaagatcgcg tagatggccg ccttatgccc ttcccaccca cgcgtcatgc gggcatggat
    42481 ataagcgccg taaatgagcc atacgatcaa cgaccaggtc tcttttggat cccaactcca
    42541 gtaaccgccc caggcgtagt tggcccacat ggccccgaga atgatgccga aagccagtag
    42601 cgggaagccc aacataatgg ccttataccc gagttcatcg attgtttcga ggtctggaaa
    42661 ggccgcatag aaacgggact gactgccccg attcaccgcc cgttccttga acagatacat
    42721 caaccccaac ccgccggcca tggcgaatgc cgcatagctg gttagggtta cggatacatg
    42781 gatgtagatc caataactat ttaacgccgg caccagcggt tctgccgtct ggtagcgata
    42841 gggcagcagc gacgctgcgc ccatcgcgat aaacccgatc cccaccacaa acgctccgac
    42901 agccttgatc cggtaccgaa actccagcag cacgtagccg agaatgatcg cccacgaaac
    42961 gtaggccatg gcttcaaatt gattggacca gggggcaaag gtcccggaat gctccatgcg
    43021 ctcgaacgcc cgggtcatca gggccatggt attgagcacc cacccaaaca cggtgaccag
    43081 ggtggccacc tgtcctaatt gagtcgccca agccccgtca cggtcttcca ggttctcaat
    43141 gggatgcccc gccggagcca tctgcatagc cggacgcttt gcgaacagat aggcaacata
    43201 gaggcccagc gctgcgagat agagccagaa cgtcatatcg aacaaaaaca atgatcgcat
    43261 catacgggcc tccgtctatc gtagagtgca gcgtcgtcct gaatggaata tccgtcaacg
    43321 gcgctgaaac gggctttaag aagaagtctt ggcttgaatc ttctccgtca atttcttgaa
    43381 ctctttttgg aagtcgatct ggcttttgtg cgtggttccg cccaaatgaa ccgtcacgcc
    43441 cgattcgccg ggaagaatcc tcgcccacag ccgccggtga tagatgaggg atgacaatgt
    43501 catccctacc accaacatcg tacacccgat ccataccatc atgatgcccg gattcctggc
    43561 aatctgcaga ccggtgaatt tcttcggttg atacccgata aattcaaact gataggcgga
    43621 atccttgatc tcaaacaggt cggggtaatg ataaaacacc cagggggtcg attgcacggt
    43681 gctgcgctca tcaaccgcca gacgaatagc aggattgcta tgttcggcgg tcttggaaaa
    43741 gaccttcttt tcagtcgaat tgaacgcgaa gtcggccacg aagtccgtca tcttcatttt
    43801 caacggaaga ccctcgaccg ctttttcctt attccactcc aaatccaccg tcgccacaac
    43861 cttgtccgac gccttatcct taatattgat ccgcgcggcc tcgatttggt cccaggcatc
    43921 cccgtaactg gactggtaaa accagattcc cttatagacc aaggggtcgt tgaccgtgat
    43981 cgtcttcgtc atggtcggag cgccttgatc gatgacggtc aaggtgctgt tgtaggactt
    44041 gacggagcca ttctcgtgat agtcgatcca gaacttatcg acccgcaggt caaagtttcc
    44101 tctgggtata tggtacgtct gcccctctag acacacgccg aactcggaaa agccgtaata
    44161 gctgccgagc agccccccaa ggacaatcac tgtcgcactt agatgggcga catgcgcgcc
    44221 gacccggccc atgattcctt tggtcgcata gacggtgacc tcgccgggat cactcttagc
    44281 cagcaccttg tagccttttt ccgcaaaaag cctgaccagg gattccgcaa ctgtttcttt
    44341 cggttgattg agcgagaggg tggtctgctg cttcatccct tgaatgaatg ccaaggaaac
    44401 actgacttta tcctgacgca tggagcgcca gacgctggga aatcgtttat ggaaacaggt
    44461 cagcgagttg acgcagagaa ggcctaagag accggtaaac caccaggtgt gatagacgtc
    44521 cgtaaagccc agacgaagaa accatcgata ggcctcttcc ccatactcct gcacgtatgt
    44581 ttccggacgc tcaccttgct ggatcactgt gccgatggtc gccgtcatgg ccagcacgat
    44641 gaacaggaac atcgccagct taatggaagc gaaaaagtct acgacttcgc gcgaaaactc
    44701 ttcccacccg aggccggacg tggaagggcc ggaggcgctc atctctgatg ctgcgtcggg
    44761 tttatcgttc atgaatccac acacctcgct gcttcgacct ggccgtcata cagatcccat
    44821 catcataacg aatgacgagc tgcgtgtggg accctgcatc catgcaggcc gataccacga
    44881 ggttgggagt gccggggcag agcgtttgat gaaaaatgtt ccaaatacgc gaaaagtaag
    44941 ctatcttaca agtcgtctcg aagagctgtc aagcaagacc cgagtctcgc gaaagacgct
    45001 atcaattccg tcagaacggt agacaaaaac cacccggtca gctactattg ccgtagcgtt
    45061 tttgcggggt tgccttatct gtgcacgtgc agcatcttcc acgctcggtc gttgtgccgc
    45121 ctaactctaa ccaccgcatc ctcgaatcac ttcacttcca gggggaacaa tgagacataa
    45181 ctacctgttt acgtctgagt cggtcactga aggacacccg gacaaaatcg ccgatcagat
    45241 ttcagatgga attctagacg ccatcatcgc ccaagacaag acatcgagag tcgcgtgtga
    45301 aaccatcctg acaaccggta tcgccttcgt cgccggcgaa atctctacga aagcctatgt
    45361 ggagattccc gatatcatcc gagacgtgat caaggatgtc ggatatacag acgcctcctg
    45421 gggattcgac tctaatacgt gctcagtcct gacctctatc catcaacaat ccagcgatat
    45481 tgcgatggga gtcgattccg gcggggcggg cgatcaggga ctgatgtttg gttatgcgac
    45541 caacgaaacg accgaactca tgcccatgcc gatcgttttg gcccatcgct tgacgaaacg
    45601 tctggctgaa gtacggaaga aaaaagtctt gaagtgggtc cgccccgacg gcaaatccca
    45661 agtcacagtc gaatacaagg atggcaagcc cttccgtatt gataccatcg tcgtctccac
    45721 gcaacacagc ccggatgtga ccaataaaca gatcgagcga gatctgatgg aatacgtcat
    45781 taagccctcc atgccgaaag gcctgtacga cgcaaccagc gtgaaacatc atatcaatcc
    45841 taccggtcga ttcgtcgtcg gcggtcctat gggtgacacc ggtttgaccg gacgcaagat
    45901 cattgtggac acctacggcg gacatggcag ccacggcgga ggtgcgttct cgggcaaaga
    45961 cccatccaag gtcgaccgct cagcctcata tatggctcgc tatattgcca agaacattgt
    46021 tgccgccggg ctggcctcga aatgcgaagt ccagctggcc tatgctatcg gcgtcgcaga
    46081 tccagtctcg gtattggtcg acaccaaaga cacggagaag gtggctcccg aaagcctcga
    46141 taagttggtg cggaaacatt tcccgctgac cccgcgcggg attatcgacc acttgaaact
    46201 gcgtcggccc attttcagga agacggccgc ctatggacac tttggtcgca acgaaccgga
    46261 gtttacgtgg gaaaagaccg acaaagcgaa agctttgcgt aaggatgcgg gcctctaagg
    46321 tcccgtcacg cgtgatacca gccgaggggg gcgggttgta agcccgcccc cttttttctg
    46381 caatcgaaga tcaatcatca ctcacgacac aggaggagga accagtggat tacgacgtga
    46441 aagatatggg cctggcggat caaggcaaac tgaagatcga atgggccgaa gccacaatgc
    46501 cggtgttgcg attgattcga aaacgctttg aaaaggagca gccgtttaga ggcattcgcg
    46561 caaccgcctg tctccatgtc acgactgaga ccgccaatct tatgaagacg ctcaaggccg
    46621 gaggggccga cgttcgcctc tgcgcctcta acccactcag cacgcaggat gacgtcgcag
    46681 ccgccctggt gaaacacgaa ggcatcccga cttttgccgt caagggggag gacaacaaga
    46741 cctattatcg gcacattgaa tcggccatcg ctcacaagcc ccacctttcg atggacgacg
    46801 gcgccgatgt cgtttcccat ttacactcaa aacgaaaaga actgcttcgc catgtcattg
    46861 gtggaaccga agaaaccacg accggcgtca ttcggcttcg cagcatggcc gagaagaaag
    46921 tattgaagtt ccccgtcatt tccgtgaacg acgcggacac gaaacacatg ttcgataacc
    46981 ggtacggcac gggccagagc accatggacg gcatcgttcg cgccaccaat cggttggtct
    47041 gtggttcgac gctggttgtg gccggctacg gttggtgcgg acgcggcatc gccacgcgcg
    47101 cgcggggcat gggtgccaat gtgatcgtga cggaaatcga cccactcaag gggctcgaag
    47161 cggtcatgga cggatttcgc gttatgccca tggacgaagc tgcgccgctc ggagacttct
    47221 tcgtgaccgt caccggtaat ctcaaggtca ttcgcggtga acattttgca gcaatgaaag
    47281 acggagcgat cgtatgcaac tccggacatt tcaacgttga attggacatc cccgcgcttg
    47341 agaaactcag caagaagaaa cttgccgtgc gctccggagt ggatcaatac accttgaaaa
    47401 atggccgtcg cgtcagcctc cttggagaag gacgcctcgt gaatttggcg accgctgaag
    47461 gtcacccctc cagcgtcatg gacatgagct tcgccaacca ggcgctgggc gcggagttta
    47521 tcgtgaaaaa ctacaagaaa ctggagaaga aggtgtaccc ggtgccggcg gatatcgaca
    47581 aagagatctc ccgcctcaaa ctcgccggca tgggtgtgtc gatcgacaaa ctcaccaaag
    47641 agcaggtgaa gtatctggct tcgtgggaaa tgggaaccta atccaaaacc cgtattcccg
    47701 tgctcgcaag cggtcggttg cggctcccgc accggccgct tgccttttct gaatggcatg
    47761 cgagccgcaa acagcgtctc ctcactaaac cactcaagac ctgagaatgc actcgcattg
    47821 atgatggctt tttcgtgctt cgatgccaat cgataggtgc atggccgata gcctcgcgat
    47881 tgcattcacg ctggggatgc tccaaactga attgcaggct tttctcaagc gtgttgccgg
    47941 acacatcagt ctaggcaggt agcagtttac ctcggtgaag aacgtgtctc gccgaagcaa
    48001 ggtcaggtct ctatggcgac tgtccgcatc ggcttgtcag ggtattctta taagccatgg
    48061 caggggccga ctcgatttta ccctccaacg ctcaagcaag cacaatttct ggagtattat
    48121 tcaacgcgat accgcacagt cgaactggat ggagtatggt ttcggctgcc taccctgtcg
    48181 gcagtgacaa aatggattga gcagactccg gctcattttg tcttttcagt aaaggcacat
    48241 cgtaccatca cccacgtgaa gcgactgagg cccgatgcgg cgtcctttct tcacaccatg
    48301 ttggagcatc tcgctccctt agccgagaaa cagaagctag gccccatcct tcttcaactg
    48361 cctcccaatt tcaaacgaaa cgatgaccgc ctagctgaat tcttgcaagg cctgcccccc
    48421 acgtatcgct gggtcatgga attccggcat gacagctgga atcatgccac tgtggaaaac
    48481 ctcttgcgcc ggtttcgcgt cgcgtgggcg gctgttgaaa cggatgatcg acccgcggaa
    48541 taccgagaca cggctgactt cctttacgcc cgtctgcgcc gcagccaata ttcgaacaaa
    48601 gacttaacgc ggtggggtga atactttcta cagacgagac ggaatgggaa ggattgctac
    48661 gcctactgca aacatgagga cgaaggctca ccctggatgt gggcagatca actgctgaag
    48721 atcatgaatg tcgggacact accgggctga aatcatgggc aactcccgag agctctcggc
    48781 ttcctgctga atgcgctggc gctccgcttt cgattcttcc agcacgctat gcaataattc
    48841 atcactcaac tgcgtcagct gcgcagccgc atctttcatc tccgcatgtt ccacggcgcg
    48901 tgagagcacc tctgccttag ttgccccctt cttctgcccc agaaatggct tgatgatgag
    48961 ataggcaaca tcgcggaatg gcatatagcg caagaatcgg cggagcaact tgaacgtctg
    49021 aattggatag cgggtgagct tgtagatgaa cagtttctta agcccctcct gccggaccga
    49081 attgatggtc gcgcctggca agcatgtagg atcgatttcc gaacacttaa aatatttgta
    49141 ccaatcctta gcatcactca ccaatccacg cttgatgtac tcctgccaca agggcgtccc
    49201 ccgatagaca cacagtcgat tgaatccaaa cgtatccaat ggaagcttcg acgcgaagtc
    49261 gaatgttttt tccatgtcct ccacagtctc atcaggattt cctacggtga aaaagccgtg
    49321 gacaatttca atgccggctt ttttagcatt tctcactgca gtctctacct cggccaacgt
    49381 ctgttccttc tgcaaacgat cgagaatttt ctggctcccg ctttcgatgc caaacatcac
    49441 cgttcggcaa tgggctttcg ccatggcggg aaacagatgc tgtgcgaccg aatccacgcg
    49501 gccttcaatt ccccattgaa tagaaagctt ggcatcaatg atgccattgc aaattgcatc
    49561 aatacgcttc ggctgcagta agaagtgatc gtccacgaaa taaaccgagc cgtacccgtt
    49621 tgcttctaaa aacttcagct cttcgacgac atgctgggcg ctgcgcgctc gccatttccc
    49681 ttcgttgaag atcggaatat cacagaatac gcagggccat gggcaacctc gggacgtctg
    49741 catggtcgta aagcgctcca tggacaggac tgctgggacg tccaacggca tggattcgac
    49801 gaagtctagc tctaaacttt cccgatccgg gaaaggccat tggtccagat gtcgctccat
    49861 ggcccgattc gggttattga tgacttttcc gtctttcgcc catgtcacgc cagccacatc
    49921 cttggggtcg tcgatatggt cgagcaactc caggagcaac tgttccccgt caccgcggca
    49981 cacaaagtcg acctccggac attgcaactt gaccaggccc gcgttcaggc tggcaaatac
    50041 gccaccgaag gccagcttca ccgagccatc cgccacgcga atctggcggg cgagaatctt
    50101 cgcgtaagga taactcgtcg tactgagaaa gctcatcccg acgagcgccg gctttcgtcg
    50161 ctttatttct tcaatgataa attcgttcgg tgtctcaggg tgcgcctgat cgaacatgac
    50221 acattcgtga cctgcccgtt taaggacggc cgagagagac ataatgccga tcggcgggaa
    50281 ccccatgacc cgaatacggc cattcttcgc tcgcgtcttg gccggaagcg cgtagaactg
    50341 aggatcacgc acatgtatga gaaacacgag catagtggcg tcctttcatg gacagttcgg
    50401 acccttaccg gacggttgcg gctccggaaa gaaaccagat ccgatcaggc gggccaacgt
    50461 tcgagggtta atcgcctcac gctgggcgcc atacagtacc acgtctggca tggagggaaa
    50521 agggaagatt ctgacatggg cgtcaaaatt cgaaggcgtc cagtgattca cccatacagc
    50581 tgcacacgtc gctaaaaaca aagagggcgt cggaccggtc cgacgccctt cagtggatac
    50641 atactggaga tgagttaccg gccaagtgcc ttcatcaccg catgcccaat ttcggcagga
    50701 ttcttgacga ctcgaactcc agcggattca agagccttca ttttctctgt tgccgtacct
    50761 ttaccaccag aaataatcgc accagcatgt cccatacggc gaccaggagg cgccgtgata
    50821 ccggcgataa agctgataac cggctttttc acgtgctgtt taatgtattc tgccgccttt
    50881 tcttccgcgt cgccaccaat ttccccgatc ataaccacag cctgcgtttc ggggtccttc
    50941 tcaaagagtg gcaacacatc cacgaagccg gtgccattaa ctggatctcc accgataccc
    51001 acacacgtcg tctcaccaag tcctaacgta gagagttggt gcaccgcctc atacgttaag
    51061 gtcccactgc gcgagacgac tccaaccacc ccgcgcttat ggatgaatcc aggcatgata
    51121 ccgattttcg cttcgtcgac cgtaatgact ccaggacagt ttggcccgat aaggcgcaca
    51181 tcacgtccat gtagtgcccg cttcaccttc accatatcat tgacgggaat tccttcggta
    51241 atgcagatga tcaatttcac gcctgcatcg gcagcttcca ggatagcgtc tgcacagaag
    51301 ggcgggggca caaaaatgag agaggtatcg cagtcggtct ttttcaccgc atcggctacc
    51361 gtattaaaga cagggatccc ttcaacttcc tggcctgcct tgccgggggt gactccagcg
    51421 accatcttcg tcccgtaagc cttgcactgc gatgcgtgga aagatccttc cttccccgtg
    51481 attccctgta ccaccactcg tgtattcttg ttgacgagaa tgctcacggt cttctccttt
    51541 acgccgcctt gccggtcagt ttgacgattt tctgcgctgc ttcccacaga tcgttggcaa
    51601 cctctaactt gagtccagac tctgccaaca gtttccgtcc ctcatctgca ttggtacctt
    51661 ggaggcgaac cactaagggc accgtaatct tcacttcttt cgccgcttcg atcacgccat
    51721 gcgcgatacg ttcgcaccgg acaatgccgc cgaatatatt gataaagata cctttgacgt
    51781 tcgggtcctt cagcagaatt cggaagcctg ccgctaccgt gtctttcgtg gccccgccgc
    51841 ccacatctag aaaattggca ggttcactgc cggccagctt gatcacatcc atcgtcgcca
    51901 tcgcaagacc tgcgccattc accatgcagc cgatattgcc atcaagcttt acataattga
    51961 gattattcgc cgtggcctcg atctccaacg ggtcctcttc attgagatcc cgcattttct
    52021 gcacatcttc atgtttaaaa attccgttgt cgtcaaacga caccttcccg tccaacgcca
    52081 ccagtgtctt ttccttggtg atgattaagg ggttgatttc gacgagcgcg gcatgtttct
    52141 ccatgaacag gcgataaaga ttaccgagca tctgcacgaa cgggttgatg acggccggct
    52201 ccatgctctg aagccccaag gcgaaggcca cattgcgacc attgtgccct tgaaatccga
    52261 cagccggatc aatcgcttcc ttaataatct tttctggggt tttctcggcg acctcttcga
    52321 tctccatgcc accttcagta ctggcaataa aggtgggcca gccggtatcg cgatcgacca
    52381 aaatactgag atagagctcc ttggcgatat tcgcaccttc ttcaagaagc aaccgatgga
    52441 ctgtcctgcc tttcggtccc gtttgatgcg tgatcagagt cttcccgatc aattctttgg
    52501 cgagccctgc gatggcaccc ctatctttcg tgatcttgac accaccggct ttccctcgtc
    52561 cgcccgcatg aatttgagcc tttacgacaa acacaggggt atttagttca ttggcccacg
    52621 cagtcgctcc ttccggcgag gtaatttctt ttccacgcgg cactggcacc ccaaactgtg
    52681 caaataactg cttggcctga aactcatgaa cgttcattga atcctcatac gagctagtgg
    52741 cttatgaaaa acgacatagt gtgcgcagaa acccagtgat gttcggctgt aaaaacgtac
    52801 gatcatttgg tagcggtacg ctctggttct ttgcgggtat aggcgggaag aatcatggga
    52861 gaaataactc cactggaatt tccatgcttc ttcgcctcag ctcgaaaccg cttcaaatgg
    52921 ttcagcgtca gttttccttg agacatggat ccggcacgtg atgcggcggt taaacccgaa
    52981 cgtttaccga agaccatcag atcaagcaag gaattgccca tcaatcgatt tcgcccatgc
    53041 agtcctccgg atgcttctcc cgcgacaaac aagttcttca cgttggtttc cgagttcgca
    53101 tcgatcttca ctccgccgtt ctgatagtgc aaggtggggt agataagcac gggatcttta
    53161 ctgatgtcga ttccgaaccg ttcgaactgc atcatcatcg cagggaaatg cttctctacc
    53221 gtcccggggc catgctccgc atcaagcaac ggcgtatcca gccacactcc gatccgacct
    53281 gacatcgtgc gaataccacg accttcctca cactcccgaa tgatcgagga ggagacgaca
    53341 tcgcgagtgt ccaactcatt gacaaaccgc tctcccttgg cgttgacaag atggccaccc
    53401 tctgaccgaa taccttccgt gaccagagcc ccgatcaact gctcagggta caccgcgcct
    53461 gatgggtgat actgaaaggt atcgatatgg tcaagcttgg ctcccatccg atacgagagg
    53521 cataacccgt caccggtagc accgtagtga ttgctggttg ggaatccctg gatatgtaag
    53581 cgaccgattc caccggtggc cagaataacc gacttggcgg cgacaatgac atgacggtgg
    53641 ttgtcgagat ccttaaatat ggctccggtg cactgaccgt tatcatcgct caataattcg
    53701 acagctgcac agaactcaag tagctgaatc ttttggttca gcacctcgtc cttcaggacc
    53761 cgcatgattt cgaggccggt gtagtccgag caggtgagaa gacgcggctt cgagcttcct
    53821 ccaccttttt tgacatgcaa attgccgtcg gcttgccggt caaagagtac cccgagttca
    53881 atcagccatt tggcaatgga tggcccctct tcaaccatga ccttgagcag ctcatggtcg
    53941 ttctgcatgt gtccgccctt gagggtatca agaaaatgtg tgaccggaga gtcttcgggg
    54001 gcaacggaga tttgcatgcc tccttgagcc atcacgctgt tggaatcgcc aagacgaagt
    54061 tttgttgcca ggatcacctt cgcaccggat gtgtgagcgt gaagtgccgc cgcacaccct
    54121 gctcccccgc cgccaataac taggacgtcg gccgtatatt gaggtgtcaa gtcgagatgc
    54181 tcggggatag agctatcgcc ttcaagcagc gccgccagct cgacaacggt tttgtcgtcg
    54241 gcattgggcc caaatcgaat cggccgatag gcactcgccc gataatcggg gtgatactta
    54301 tgaattaact ggtcgcggtc ggcaggggaa aactttggaa tggtctgctt gcgacgagca
    54361 tctcgggttt ggtgaacaat ctgctggagt gcatgaatat ccatggtttc actttcagcg
    54421 cggagcacag tacccgagcc atgcgctcag aaccggctaa cggcacaatg cattacttta
    54481 cggtcgcaca gtgatcggcc aactcctgtt cgttcatcgc gagcactttg ttccactcgt
    54541 cgttgaatcg accgtcttga atttctttaa tgcggagatc aaggccctgg ggcttctcgg
    54601 taaaatgcgc tccttgggcc cggctgacat ataaggccac caggtttggc gcgatgtcgg
    54661 caatacagac gggcgtgcac atgccgcaca tgacacaatc cataaacatt tctgacacgc
    54721 ttttgaagtc gccaaatact gctttccaga cgccttcacg cacatcaatt ttctgggggc
    54781 aggcttccgt gcaagcgtta cagtttctgc agagcggcgc ctcggggtac aaattgaaga
    54841 ggtcttgctt gggatcttta agcgactgaa tgtcgtaaag cgctttgcgg gctggaaacg
    54901 ggggcatgat ggtaaaggac atgccgtctt caaccgccat ctgacaagcc aaacaggttc
    54961 gaaccttcgg atcgtccttc gtgcggtaat atgttgcaca ggcgccacag aaaccaccga
    55021 gacaccctgc gcctctcacg acctcctggc cagtatgcca caaggccttg atcaccgtaa
    55081 taccttccgg cacttcgtac tttttaccgg aaatctcaat ggtcaccatt ttgtggcgca
    55141 atacttccgg ctgatcgatc acattggtgt cttcttgtgc catggctttc tcgtcaatcg
    55201 taaaaaggtg cggcgcaaga aggacgttgg tccttcttgc gcctgaccgg atcaaatcat
    55261 gatcgcttaa gcaatcgcgt caatgattgc gttcagcgtt gcgcttggcc gcagggcttt
    55321 agcggctttc gcatcgttcg ggtggtaata cccgccgacg tcctggggct taccctgcgc
    55381 ggcaagcagt tcgccgtcga tcttcttctc gttgtcgctc aaatcctttg caattttcgc
    55441 aaatcgctca gcgatcttct tgtccgcggt ttgggccgcc aacgcctgcg cccagtagag
    55501 cgcgaggtag aagtgactgc cgcggttgtc gatctcgccg accttacgcg cgggtgactt
    55561 attgctctca a
//
LOCUS       DAOQDS010000023        53330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_197_length_53330_cov_20.026222, whole genome shotgun sequence.
ACCESSION   DAOQDS010000023 DAOQDS010000000
VERSION     DAOQDS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 53330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53330
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197_length_53330_cov_20.026222"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2756)
                     /locus_tag="PK589_07450"
     CDS             complement(<1..2756)
                     /locus_tag="PK589_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ19474.1"
                     /translation="MKATDMFSWLTGSEMGGRIRAFDWSRTPLGPIEQWPASLVSILG
                     VCLTAQTPMAIYWGADSWLLYNDAWRPVVGGKHPWALGHPAREVWPELWPTIQHYFHS
                     VLNTGEANWRTDELLPMQRFGYTEECYFDYSLNPIRGQSGAVEGILNIVQETTYRVLN
                     DRRTSLLRELASLSGSAQNDTDACARILQALATDSADVPFALLYQIDRDHREAQLAGS
                     IGLEDASLARQEQLDLTGNAAPGSWPLAAAIRNGGPLLLDELPDGFGLLPGGAWPEPT
                     RQCLLLPIGTATQDGGVVLVAGISPRRPLDEHYHHFFRMVSSHIAVAINNARAYQSEQ
                     RRAEALAELDRAKTAFFSNVSHELRTPLTLMLGPLEEELHLRPQSPALEVAHRNSLRL
                     LKLVNTLLDFSRIEAGRAEASYQPTDLAAYSADLASGFRSAIEKAGVTFVVDCPPLPE
                     AVYVDRSMWEKIVLNLLSNAFKHTFQGQITISMRHDAAGAELVVSDTGVGIPDEALPR
                     LFERFFRVQNRRSRTHEGSGIGLALVQEVVRLHGGSVQVESRLDEGSTFTVRISWGTA
                     HLPAEQIRAGDEPSVRSASAVAYVQEALHWLPDTQAIPPPASTSEAASAVIRPRVLVA
                     DDNADMRQYIERLLSRRYDVVTVGDGLAALNEIRRHPPDLLLSDIMMPQLDGIALLKA
                     LRADPAVHTLPVILLSARAGEESRVEGLEQGADDYLVKPFSAQELMARVTTHLDLSRI
                     RRKAEQEITRSKLFLERIAASTPDLLFVFDLLRTRTVYINHSLKSVLGYDVEQLAATQ
                     DNPIVTLVHPDDVPSVEQWLGRFDSAADAEVLEHKHRLRHADGTYRWILMRASVFDRS
                     PEGRARQIIGIAKDTTDQIRAEELMAASEARYRAIVSQATASVAQTDLTGRFVFVNQR
                     FCDIL"
     gene            complement(2989..3456)
                     /locus_tag="PK589_07455"
     CDS             complement(2989..3456)
                     /locus_tag="PK589_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19475.1"
                     /translation="MMLNTLMGSGLLTVVLLTGGQAVQQDVPAPVPNPGPGPTVPPKP
                     SDPIPTPPTVPKLLLQAWSGVERTSLFLIAEAAPRSQEAVSGKLLHLDLEKKEGRLGT
                     DLGREVPFRIPKPELFVHLSVGQRVTIKLDSGQQAVGVMETAAPELPSPSPAR"
     gene            complement(3479..3889)
                     /locus_tag="PK589_07460"
     CDS             complement(3479..3889)
                     /locus_tag="PK589_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HNJ19476.1"
                     /translation="MRMSTSIQSVDQHRLDCIQACLDCARICNTCGNDMIGMDMSEDE
                     EAQDLMVRCIRLCRDCADICLLAAQWMGRTSAFAVRLCAFCVEMCELCAGVCEQHAPH
                     HAMCGDCAKECRRCAELCHKMVLESVPASTGGTA"
     gene            4044..6275
                     /locus_tag="PK589_07465"
     CDS             4044..6275
                     /locus_tag="PK589_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNJ19477.1"
                     /translation="MANIAMHNAAEGGALSSPPTEPEGSSPILVPGHTCWRIEPAERI
                     AFLVDGEAYFQAFREAALRAKHSMMIVGWDIDTQVELVRETSASSEMPTKLGEFLVAL
                     LRRRRRLRVSVLNWDYAVIYALERQWMPTAEPGWRRHRRLSYQVDDHHPVGASHHQKL
                     VVIDDAVAFVGGLDLSKSRWDTREHLSRDPRRVDAEGIVYAPFHDVQMMVSGDAASAL
                     GDLARNRWFNATGRRLSAPPPRSVEEVWPPRVNPDLTECPVAIMRTQPRYEELEEQRE
                     IEQAYLSAIKRARRSIYIESQYFTAQAISHALAQRLSEHDGPDVVLVLRQHCDGWLER
                     RTMDSLRAQLLHEVELADHYGRLRVYAPVVPGEAGAEPVAVHSKLLVVDDEFVCIGSA
                     NVSNRSMGFDTECNLAVEACGQARVQSAIAAFRNGLLAEHLGVDPEVVANQIQTCGSL
                     AAATDALRGGGRTLADDCFEAMPPSDAMIPEHALVDPERPMAADHLLAGVGFRRERSR
                     IVRRAMAGLTLLAGMAILAAAWRWTALGQLLDVEALVSYAQALGRSPLGLISVMGGYL
                     IGGLLAVPVTLLLVVTILAFGPWFGALFALTGSLLSAITLFGLGRVLGSYRIRRLAGS
                     RVEHLSRRMAERGLWAVLLVRILPIAPFSVINLIAGASSVSWRHFVLGTVVGMLPGIA
                     IMATFVSGLEEAVREPSWQAFSMVGLVVAAAWAGVWYVSSRLQSRQSTESVMAAGSSV
                     PAT"
     gene            6301..7071
                     /locus_tag="PK589_07470"
     CDS             6301..7071
                     /locus_tag="PK589_07470"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNJ19478.1"
                     /translation="MRVQVASYNIHRGVGRDGRYNPGRILRVLQEVNADIVALQEVDF
                     PGPHPHHFLTWLAAQLGMTAVGGPVRTSAAGTYGNALLTRYPVRNVRRWDLSVGRHEP
                     RGALAADMLCQGAMIHVVTTHLGLWPTERPRQATKLLEGLHVTEEELTILMGDLNEWQ
                     LWGKSLRVLRKVFGAPVSPPTFPTAWPLLALDRIWVSPPRALFEVHTHQSRQSRTASD
                     HLPVKAEIRFESVHGTGSVMREDYEPTPTSTGCGRSML"
     gene            complement(7023..7481)
                     /locus_tag="PK589_07475"
     CDS             complement(7023..7481)
                     /locus_tag="PK589_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19479.1"
                     /translation="MKTEMKKFIPICCVCENVRGETLSPGTEPEWGPLKTYLDTHHLT
                     IGGYRLTHTYCPACAVQFTKLGRTARPAVTEEQPEIDNSVTPTILDAFDHMQQCDMDM
                     LVEACPTLTWNQVFSEVDRLSRMGQLRLSHLGYGRYSIERPQPVEVGVGS"
     assembly_gap    7500..7599
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7779..9419)
                     /locus_tag="PK589_07480"
     CDS             complement(7779..9419)
                     /locus_tag="PK589_07480"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNJ19480.1"
                     /translation="MSIRIDSRQTTYTGESLHAASSVKKHHRGMWRWVMAACAAVILL
                     GGIGLWFVDEPLRVYAERQLNGRIAGYTFHIGTLDFHPIGLSVDLENVTVIQEAHPDP
                     PLAHIAEWHASIQWEGLLSGRLVSDHIIDRPLIHFTRPQAAQELQDAPAQKKSWQEAL
                     FAVYPLQINELSIKDGDVTYRENATSNPIRLTNIQVLAENIRNVRSKPLQYPSRVQVE
                     AMVAGGGHMKVDGHADFFAEPSMGVDVDITLQDINLADLLPLTAQRQIHLTQGSLTTS
                     GHVEFAPAAQEVRIASLNLHDVKVDFVHAARTKQREQRTAQQVSRTAAKAANHPTLLL
                     RIDQGKVERSEFGFVNQATNPSYRLFISGTELELENWSNQLSEGTARVQLKGMLMGTG
                     ATKITGAFRPEVESPDFDLSVKIVKTQVKSLNQLLRAYGGLDAAAGLFSLFSEVNVKN
                     GTVKGYLKPLFKDVTAYDPQQDRDKGMLNQLYEKTINALAEILKNTPRDEVATKTDLS
                     GTVENPQASTWELVLTLFQNAFFDAVLPGLEGKVQLER"
     gene            9772..10845
                     /locus_tag="PK589_07485"
     CDS             9772..10845
                     /locus_tag="PK589_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNJ19481.1"
                     /translation="MAHTWKTLLQPQSLWKLLQEAFTAWSDDKIPRHGAALAYYTVLS
                     LVPLLIVMISLIGLIFGREAAQKHILDQTTNLMGPQSGDAVKQMIEHATQPTTGTIAM
                     LIGVATLLLGASGVFAQLQDSLNTIWRVEPKEGRGLWGLIRDRFISISAVLGTGFLLL
                     VSLVLSAALAAFGKWYSGWLPAPEVVLQLLEIVISLLVIAGLFALMFKVLPDARIAWN
                     DVWVGALLTAFLFTIGKFAIGLYLGKSDVGSAYGSAGSLVIMLVWVYYSAQIVLFGAE
                     FTQVYANELGSRIVPTENAVPADEPRAGRMRPAPTQPRLQPASGRQAQAASAQRQPAE
                     RLPWWGTVLLLWVAVTRFPRRSE"
     gene            10961..13042
                     /locus_tag="PK589_07490"
     CDS             10961..13042
                     /locus_tag="PK589_07490"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNJ19482.1"
                     /translation="MAGKQDMKTNQGHGGETHQQAGGPKEPVLTTDQGIPISDNQNSL
                     KSGARGPTLLEDFVLREKIFHFDHERIPERIVHARGSAAHGFFEVYESLSSLTSADLF
                     QRKGERTPVFVRFSTVVGGAGSVDTPRDVRGFAVKFYTKTGNWDLVGNNIPVFFIQDA
                     MKFPDLVHAVKMEADSGFPQAASAHDTFWDWASLMPESTHMLMWAMSDRTLPRSFRMM
                     EGFGVHTFKLVNDKGKETFVKFHWKPKLGLQSTVWEETLKLQAADNDYHRRDLREAID
                     AGNFPEWELGIQTFDQAFADKQPYDVLDATKLIPEEQIPVRRIGRLVLDRNPDNHFAE
                     NEQVAFCPANIVPGIDFSNDPLLQGRLFSYLDTQKSRLGTANFHQLPINAPKCPMANF
                     QRDGQMQMRVPTGRANYEPNSLAAHGEEGGPRESPQGFTTAPGRDDREEQGDKLRVRP
                     ELFADHYSQARMFWKSLTDNEQAHLASAFVFELAKVNLDEVQSRMVGRLRNVDEGLAK
                     RIADGLGMPLPGKIRAAREPIEMKPSPALSIQKNMLATLKGRKVGLLYAEGSNGGDID
                     RMIKQVEKAGGTVFLVAPKLHGTKLENGKELKADGQLAASPSQLFDAVAIILSAKGAQ
                     MLLKEGAAIQFVMDAFGHLKAIGHSEAAQPLLDKAGVVPDEGVTDLRGSFVEAAAKRF
                     FAREPSVRTLA"
     gene            complement(13157..13594)
                     /locus_tag="PK589_07495"
     CDS             complement(13157..13594)
                     /locus_tag="PK589_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19483.1"
                     /translation="MKTLMHSLLALALVSLAACSSSPTASKTRSEMDVGTKTGMVKNI
                     VIKDTLTPDALTVRAGDEVRWINQRQDSVTVTIDGPLEHNVSCRNGFSKALGMGVDNS
                     TTLASNETAGLCFSRVGTVNYSVRPSSDTRTTMADTKGSITVQ"
     gene            13801..14682
                     /locus_tag="PK589_07500"
     CDS             13801..14682
                     /locus_tag="PK589_07500"
                     /inference="COORDINATES: protein motif:HMM:NF039714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HNJ19484.1"
                     /translation="MSPSEIIRKSGGSAFHRLTGLLLTLVLLLSGCALSFGYRHADWL
                     ITRQLDHYLDLTSSQRRIVNARLQPLLWRHRIEALPQYEQFLKDLHARIGRGLTSEDV
                     EWTFASYDRFRADLFERAIPEGTQLAMLLKNNQITYLERVFRKEEVQAESLARRSVST
                     RLDERARKGIATAEEWLGPLSHEQSVQLREMMLALPDSQPTWWQYRRQRHGEVLAMLR
                     TSTSADQIAERLRYLFVQSDQTAPTGYLKMMTEMRAALTRMVLDVDRMLTPAQRRHVL
                     VAIQKVIDEVHALAHNS"
     gene            complement(14964..>15791)
                     /locus_tag="PK589_07505"
     CDS             complement(14964..>15791)
                     /locus_tag="PK589_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19485.1"
                     /translation="TAQNGPSLTEILTALANPQTHVVVNLVGLPLHDRPAFFFGLLHK
                     ILELRAASGRPHWILIDETHHLLPRDGNPGAPGLTQDCTGMMYVTVHPDQIDPAILKT
                     VDLVFALGQSPGKTIEAFCQARGQQAPTLPSTELEAGQTLMWDPTLAVAPEVLEIAPS
                     STERRRHRRKYAEGELPPDRSFYFRGPAGKLNLRAQNLLLFMQLGEGVDEQTWQYHLN
                     AHDYSTWISDMIKDETLSKVVHDIEARPELSAEESRRLIRAAIEEQYTVPATGIEPA"
     assembly_gap    15792..15891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15892..16373)
                     /locus_tag="PK589_07510"
     CDS             complement(<15892..16373)
                     /locus_tag="PK589_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HNJ19486.1"
                     /translation="MRYMALAADYDGTLASDGAVYPETVAALERLVASGRKLILVTGR
                     ILPELLDIFPQISLCELVVAENGGVLYRPATKEVTLLADAPRPAFLEEVQRRAVQHLS
                     LGHVIVATRVPYETVILDIIRDLGLELRIIFNKGAVMVLPHGINKATGLTAALARLEL
                     S"
     gene            16520..17500
                     /locus_tag="PK589_07515"
     CDS             16520..17500
                     /locus_tag="PK589_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19487.1"
                     /translation="MSYSGRIHLIAGWCGLVLIVGGCTTVLPPADHGAANESSQRCVP
                     AMTKRARVAAAGDETPVTPTTEARSRFSSDALQVAEVVGVLPLVNHLASDTGSSRRDP
                     LASVVLRQRLTEILLLTLFEITSTTAELTCERDRADQVADRIDEIDGARVKRLTIASI
                     LFGGIASIVSGGIGLAAGASVGGEAADVAGGALASWFGVSALFTHSEVEFRHERNVLQ
                     EIWEDPSQPRLFSPTIWRYLHRAHDEKGKLPREQVLDSWQQAGRMGENGSPSEQERRR
                     LFFGAGGRYGAAELHARASMLETLEASIRLLYEELEVLMRELAEETADVS"
     gene            17523..17963
                     /locus_tag="PK589_07520"
     CDS             17523..17963
                     /locus_tag="PK589_07520"
                     /inference="COORDINATES: protein motif:HMM:NF016045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HNJ19488.1"
                     /translation="MVSKHTKTRDSGLFVRFANEASRVVGSAGAFGVAIVVVLLWSLS
                     GPLFGYSDTWQLVINTGTTIVTFLMVFLIQNTQNRDSAATQLKLDELIRSTQGAHNAL
                     LDLEKLSSKDLEDIRMLYQKLADRARREKKGGWEDTDTPELKRV"
     gene            complement(17970..19523)
                     /locus_tag="PK589_07525"
     CDS             complement(17970..19523)
                     /locus_tag="PK589_07525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ19489.1"
                     /translation="MHDEPGRTVSIWMSENIPQLPPISTGAHADVCVVGAGISGITTA
                     YCLMKEGRSVILLDDSQPGSGMTERTTAHLSNAIDSGYLDIERLHGRSGARLAAESHT
                     AAIDWIEKTIKDARIDCDFLRVDGYLCAASPDDAQHIKDEWMAVLRAGLSEVEHVTRP
                     AGPLAKNGPCLRYPHQGQFHPLKYLAGLVKALQSGGCRAFSGAHVTKVESGKQARVET
                     SQGCVVTADAVVVATNTPINNMVAIHTKQAAYITYVIGAPIPAEAIEQALWWDIQDPY
                     HYVRTQRHSFPDGREELLLIVGGEDHKAGQADDAERRYGRLETWAREYFPAMGAVSYR
                     WSGQVMETVDGLAFIGKNPGDVDNIYIATGDCGMGMTHGTIAGLLLTDLIMGRECPWA
                     SLYNPSRQPVKAMGEFVRESLNVVAQYTDWVTKGDVEREEDIANDQGAVLRDGLTKIA
                     AYRDADGTLHTCSAVCTHLNCIVSWNHSEHTWDCPCHGSRFDRFGKVLNGPAITDLAP
                     VKRKERTPT"
     gene            19916..20776
                     /locus_tag="PK589_07530"
     CDS             19916..20776
                     /locus_tag="PK589_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HNJ19490.1"
                     /translation="MPRSLWKGSISFGLVNIPVVLYSAENRNSFDLTLLDRRDMKPVG
                     FKRYNKESGKEVTWDQIVKGYEYDKNRYVVLTDEDFKRANVEATQTIDIVNFVRAEEI
                     APTSFETPYYLAPDKHGEKGYALLRETLKKTGKAAIATVVIRTRQYIAALIPWSEMMV
                     LNTLRYANEIRSSKELDLPAKHEKSAQVTPREVDMAMKLVEEMSEPWKPERYKDTYHE
                     DLMRLIEKRIKAGKTEVVTTPGKEEGGEKPGRAKVVDLMALLKRSVQQSGHSKHPARE
                     KERSPRRKSA"
     gene            20815..22800
                     /gene="treZ"
                     /locus_tag="PK589_07535"
     CDS             20815..22800
                     /gene="treZ"
                     /locus_tag="PK589_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HNJ19491.1"
                     /translation="MAGVSKAEPEAETELVKDSHSREEKQGLTLGAQVTDKGVRFRCW
                     APRAARVDVVLEGRNVIHALSKQADGYWTGLVPEASAGMQYRYRLDGGQAYPDPCSRF
                     QPEGPHGPSLIVDPGAYTWHDDRWPGVAMHGQVIYELHIGTFTPEGTFEAAIERLDGL
                     KEIGITVIEVMPVAEFPGRWNWGYDGVGLYASAHVYGDANSFKRFVDEAHRRGLGVIL
                     DVVYNHLGPDGNYLPTFSAEYFTDRYPNEWGQAINFDGPGSGPVRDFFIQNACYWIRE
                     YHVDGLRLDAVHAFHDASPVHVVAELSRAARQAAGPRSIILVAECEAQWVQTIQPLAE
                     GGWGLDGVWSEDFHHTVRVAATGRSEGYYNDYRGTPQEFISCVKRAFLYQGQRYQWQG
                     KPRGTVVSEEPAEGFVFFLQNHDQIANQLWGDRLHQKVSPSVYRALTALLLLAPETPL
                     LFMGQEFAASSPFLFFTDFPPGQLANDIHQGRKQFLSQFPSYASSAAQDAIGDPSAPA
                     MFERSKLDWTERERHAGIVALHRDLLRLRREDDVIAQQRRDRVDGAVIGPSAFLLRYA
                     GQHGDNRLLLVNLGPDLDFRPAPEPLLAPPSGRMWTFRWSSDAPSYGGPGVIEPLTET
                     GWKIPGGAAFLYAALDDTSKDTGKTPAMASPHEEESR"
     gene            22797..24839
                     /locus_tag="PK589_07540"
     CDS             22797..24839
                     /locus_tag="PK589_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNJ19492.1"
                     /translation="MTTMADAMVLTVPPERLRDPNAFLAREWLVTNGLGGYASQSLLC
                     APTRRYHGLFVPDLPAPWGRTVMMPRFDDEVLVDGDAIFLSGVEFSDGRLESNLLSVF
                     DGFSRERQTPVWRIRVKGRRLEKRVIMPYGHNSVYVEYRLLEGDPVGLRLRPFVTFRM
                     LDARLHDAKRPPFPLTVLNGRYDMHLCEGAPSLKLCLRPHGGKFVADYELSVDVSYRV
                     DRDRGSEHIESLASPGYFVADLTQDRPLAFVASTEPWELLEFDPEAIFEAEAQRLDKL
                     LSQRGGEADEDIEGWLTMAADQFVVAPGSRLEEQALARASGNEARTIIAGYHWFTDWG
                     RDTMISLEGLTLCTGRHQEARAILRTFANYVKDGLIPNLFPEGERMGLYHTADATLWY
                     FHAISRYYRVTNDRETLLTLLPVLRSVLEHHVRGTHFGIGVDAKDGLLRASAEGYQLT
                     WMDALVDGWVVTPRRGKPVEIQALWYNALRCMEEWEVLAGQTPSRWRDLAEQVQRSFN
                     ERFWIAAEGYLYDVIDGERGDDTAFRPNQVLSIALPHPVLQEDRWRRVVDLAADRLLT
                     PVGLRSLSRDHPDYKPMYFGDLRARDAAYHQGTVWAWLIGPFIDAWLKVYKDKSKARG
                     MLDGFRSHLLDDGIGTVSEIFDAEAPFHPRGCIAQAWSVAEVLRAYRNTRAEKERV"
     gene            24852..25574
                     /locus_tag="PK589_07545"
     CDS             24852..25574
                     /locus_tag="PK589_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNJ19493.1"
                     /translation="MARIGTSGWHYAHWRGPYYPPSVPPRAMLDYYARDLDTVEINNS
                     FYRLPPSGTFEGWKKSTPPGFCFAVKASRYLTHQKKLLDPSPALKRLLGAAVHLGAKL
                     GPILFQLPPHWRCNLERLSDFLRTLPPSHRYSLECRNATWHTGEVYRLLEQHNVAFCL
                     FELGGCMTPIEVTADFVYVRLHGPGNKYQGDYPTATLKAWCRRIRQWTRCDKDVYVYF
                     DNDQAGYAIKNAMSLRAMVDGS"
     gene            complement(25623..26990)
                     /locus_tag="PK589_07550"
     CDS             complement(25623..26990)
                     /locus_tag="PK589_07550"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNJ19494.1"
                     /translation="MEMEALQIPMLYGRVAIAVAALAHALCATFIVGSSVIGSATETI
                     GHVTQNLRFERLSRLIAFTLILTTAGISFLGVTFVFLLNIFWPRFWSQLFHIMFWPLL
                     LEASFFLAEAIFAYTWYYSWDWANQQPARKRWHLVFGWSAAASSLIAMLMIDMVASYM
                     LTPRPPDDTWAKLFNPTMLYLDTHRIVGNLTWTGFGLAGLCAAGFLRSGGEDDRAFYR
                     WAGGVCFIIGFGALLIMPAIGYQYLLRVRHIEPQAFYTLMLGARSWLFDLVATLYSLL
                     VLFGSWYIWRSVRAQPANDEGGTARLALPMSLLIVAIAGMILSQPYQLQHIPGMQILT
                     DRLINPLGKMQPNKYFALAFLVVFGLMNWLFFFRSYANRVPWWQGQSGSEKESSSPAL
                     LITLALCAMLMMLTMGWARETARAYNGYLIYGQVRLEDERSTYEGMKTNPGTPDAATR
                     RPVSP"
     gene            complement(26990..28207)
                     /locus_tag="PK589_07555"
     CDS             complement(26990..28207)
                     /locus_tag="PK589_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19495.1"
                     /translation="MINYGTVLPAIQIPLLGNSVAVGLFSLLHIVPAALSVAFMLMAP
                     VFEFRGRTDPFDRELALLVTRVTVVVFSVSTVLAVIMVELMIGLFPVTTMWMWNHFRV
                     PILFGVASFMLMLLALYPYYHFWDRLRATSITLHMVLGALAAFFMLPWVFMLDGMGAF
                     MLTPVQEGGTWERLFTPTWLPLVIHRMVGNLMIAGYTLAAYGAWRVWSPGEPALKPYY
                     QHLLSRGWVIGLVAFLLQPFTGLLYAMAIHRAAPDAQASIMTGPYVPWLYLQLLLLSL
                     FFLGNDVLTKRMRTLTGNRQRWLDIGFPVLAALLLLSAGYPELRRVCVYLLVGVTVWR
                     VAASVSKAPTESALSPSPVRSLAVGLALLSFLLYLTMGVMREASRRPDTVRGIISLQD
                     ELRHQARMEGALR"
     gene            complement(28200..29234)
                     /locus_tag="PK589_07560"
     CDS             complement(28200..29234)
                     /locus_tag="PK589_07560"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNJ19496.1"
                     /translation="MSRTQPCEGLIALFPLDADLDSVRLSLEGSGISQDQIALLTPVP
                     LKSASATDGTSQPLWPYIITIVAGLVGIGVGVFFAAGTALLYPLMTGGKPIVSAPIVG
                     IISYETMMLLAIVVTFITMIVRIRRARRASVDRETRIDDGKIALSVRLVEDGQEQRVK
                     TLLEMAGAEDVRLVTHPSPIQQGMQRAHVTAAWLLICITVGGCSPDMQEQPSYRSQEA
                     PRLHSPTGSVPRDSRAILSREARTMELEGGRAPELYRINCLPCHGEGGEGTGLVAPYL
                     HELPANLRSPRVRALSKTELYRIITQGKDMMPSFQGELSATERMILASFVHAMEPDTT
                     MAHEERRSHD"
     gene            complement(29185..30546)
                     /gene="nrfD"
                     /locus_tag="PK589_07565"
     CDS             complement(29185..30546)
                     /gene="nrfD"
                     /locus_tag="PK589_07565"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNJ19497.1"
                     /translation="MNHSTAGWEEMPAINRDLLAPLSAGGRGYVLLVTLLAAMVSAGI
                     GAWVYQIETGIGRTNLHPPIFWSAYIASFVYWIGVSHSGTFISGVLRLTKAEWRRPIT
                     RVAELMTIISVVIATLFVFYHLGRSWRWYYLIPYPNQREIWPDFRSPLMWDATAVFTY
                     ATASTIYLYLPLIPDFALVRDRIGGWRKPLYSLLSLGWRGSQREWTALNTAIRIITPL
                     IVMVMISVHSIVGWDFGMSLVPGWHTSIIAPYFVVGAVHSGLGMVVIGLYLVRRIYRL
                     DAYIRLEHFDKLGKLMIVMTLTLAYLYFADQLTVWYGAIPDHLSVMHAVMSGPYAVPQ
                     WTMITLIYVLPLITLTIPAFRTWPAGMLVIGLCINIGMYIERMLILTPPLAHPRLTYN
                     WSDYFPSWVELTIMAGSLSLAVLLYVLAIKFVPIISIWEEKEGRLHEQDSALRRPDRT
                     LSA"
     gene            complement(30543..31265)
                     /locus_tag="PK589_07570"
     CDS             complement(30543..31265)
                     /locus_tag="PK589_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNJ19498.1"
                     /translation="MEEKQRPSDEPRPYRWQMVIDLDRCTGCEACVVACHAENNIRIS
                     GPGEASDGRAINWIRIERYWEGAYPDVKARFMPVLCQHCADAPCEPVCPVYASYHTPE
                     GLNAQVYNRCIGVRYCGNNCPYTARQFNWFDPHWDPPLEQQLNPDVTVRQNGIMEKCT
                     FCVQRIRQGKEQAERDGRPVRDGDITPACAQSCPTTALVFGDGNDPTSRVATMAKSGR
                     GFHLLESLGTHPAITYLKRLDE"
     gene            complement(31318..33534)
                     /locus_tag="PK589_07575"
     CDS             complement(31318..33534)
                     /locus_tag="PK589_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNJ19499.1"
                     /translation="MDVKRRTFIKALGLTAAGTLLPGCDQEVRRLVPYVLPDDEIIPG
                     VAEWYASTCGECDAGCGIIVKVMEGRAKKIEGNPLHPVNQGKLCAQGQAALQRLYNPD
                     RLRGPLRRMGTEADQRFTPVSWDEGLHQLADQIRHAKGPVIMISRPLSGTLADVVSDF
                     VASVKGSLYFYDPSPELALRAAMKQAFDIHDLPAYDLGDADYVLSFGAPFLEQWLSPV
                     SFGIAFGRMRQGRKNRRGRFVHVEPRLSLTAASADRWLPIRPGMEGLLARGIGHILLS
                     EHRSSLPTGRLHAYESLYGRLSLDEVAKQTDISTADIKELARAFAEAVSPLAIGGGTA
                     CAHTNAFETHLAIHGLNALAGNINKAGGIRWYQSASLTLHPEIPWLSERRIKELAETI
                     EKTGQRPLLLLYDCNPLFSVPPAIPIRRLFDRASYIASFSGSLDESTVSADLILPDHH
                     RLETWGDHVQGGSSPYRAASLVQPVVVPLYDTRGLGDTLLELTRQLAGKHAGVESFPE
                     HIKKRWQHFHTSHSSGSHEPFQSVWEQLLQQGGWWDSAANEQRLQQSSSAVEYRPPEF
                     DGDASEFPFYLYPFAAPHLGQGRGANLPWLQELPDTMTSAVWGSWIEINPATAQAMGI
                     QQGALVRLHSRHGSLEAPALHYPGLRPDVIAMPIGQGHTDYGRYAARRGANPMTILAP
                     LFDKQSGALAIGATRVRAEPLNVPGTLILLEKPGTTAAGSSDLISIERPPSSKART"
     gene            complement(33572..34090)
                     /locus_tag="PK589_07580"
     CDS             complement(33572..34090)
                     /locus_tag="PK589_07580"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNJ19500.1"
                     /translation="MTRSLWQSLGLVSLATVSALAIGILYSKGSADSAAHDQQPIAFN
                     HAFHAGELALDCRYCHRAAHISPAAGIPSLQLCMGCHQNLDVATAGIQTLVDHWKRQA
                     PVEWVRLQRLPDFVYFTHERHLAQGLECTTCHGDVTRMTSTPRAATYEMGWCLSCHQQ
                     RGASRDCVTCHK"
     gene            34371..35492
                     /locus_tag="PK589_07585"
     CDS             34371..35492
                     /locus_tag="PK589_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNJ19501.1"
                     /translation="MERDVCSTNGDSGPKIEKVQVSAYTIPTAGPESDGTLEWDRTTL
                     VLVDVSAGGQHGIGYTYADVSTAWFVKQNLAPVVEGRDAMHIADAWWAMMRSVRNLGH
                     PGVASMAIAAVDTALWDVKARLLDLPLVSLLGRVRTAVPVYGSGGFTSYSIDRLEEQL
                     GGWVRDGISRVKMKVGRQPDQDLRRVEAVRKTIGEEAQLFVDANGGYGRKQALQLSEG
                     FAKAGVVWFEEPVSSDDTEGLRLLRDRVPAPIQVTAGEYGYDLFYFRRLLDAEAVDVL
                     QADASRCGGITGFLKVGSLCEARPVPLSAHCAPSLHVHPGCASMSVCHIEYFYDHVRI
                     EQLLLDGAIRPVGGALYPDLSRPGIGLDFKYQDARRYQV"
     gene            35494..38502
                     /locus_tag="PK589_07590"
     CDS             35494..38502
                     /locus_tag="PK589_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ19502.1"
                     /translation="MREPLIHIQSAGQDSTQGASYAAEPSDAMREHLARRIDAQGLAE
                     ALRKNIDGEVRFDEGSRALYATDASNYRQVPIGVVIPRHADDVLRTIATARTYDAPIL
                     ARGGGTSLAGQCCNYAVVLDCSKYMHRLVGIDVAGRSARVQPGLVLDDLRHAAEQHHL
                     TFGPDPSTHNHCTLGGMIGNNSCGVHSVMAGETSENVIEMDVATYDGLRLRVGRTSDH
                     QLDEIIAHGGREADLYRRLIALRDRYADLIRSRFPNIPRRVSGYNLPALLPEHGFDVA
                     KALVGSESTCVTVLEATVRLVSSPRVRSLLVLGYPDVYAAGDHIPDILRAKPIGLEGM
                     DDRLVEDMTKMKIHPDDVKLLPEGRGWLLAEFGGESKDEADGQAHRCMELLKAAGHPP
                     SMKLFDRRRDEQVIWTVRESGLGATAHVPGEQVTWEGWEDSAVPVERLGDYLRALRRL
                     FEKYRYACALYGHFGQGCVHTRIDFDLQTKEGISRFRSFLFEAADLVKSFGGSLSGEH
                     GDGQSRAELLPRMFGPELIGAFEEFKAIWDPDGKMNPGKIVRPFRVDENLRLGSDYHP
                     PDPQTDFTFPQDEYSFSRATLRCVGVGECRRQAGGTMCPSYRATREELHSTRGRARLL
                     FEMLEGDPLTRGWRNDTVKEALSLCLACKGCKGDCPVHVDMATYKAEFLAHYYKGRLR
                     PRSAYSMGLIHRWARWASRAPGMANFITQTPGLCDVVKMLGGISPHRRMPRFAAETFQ
                     AWHRQRAKRAGGGQPVLLWPDTFNNYFYPETARAATEVLEAAGCAVFVPQTNLCCGRP
                     LYDFGMLRQAKRHLQQILCELASTIRSGMPLVVLEPSCAAVFRDELGNLFPHDEDARR
                     LAKQTFTLAEFIDKRLPDWELPQSHQSVTVHGHCHQKAVIGLGAEERVLDRLGLNVQM
                     LDTGCCGMAGSFGFKQEHYDISMQIGELALLPAIRNTATDTIVISDGFSCRQQIEQST
                     HRQGMHLAEVIHQAMGNVTMHVSASAHGGT"
     gene            38549..39184
                     /locus_tag="PK589_07595"
     CDS             38549..39184
                     /locus_tag="PK589_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ19503.1"
                     /translation="MTSSKTLIAVVLICLIGTGMLNSRPVLGEWYVAGQFGANFADQL
                     SDIHGTGNLSGFRAPDFDLQNSLLYGAKVGNFPGNGWFGLELDVFHSTPHVKNLDDVP
                     GFHMRVTNVGVNLLARYPGVTFQPYAGIGAGVAIARIGDSATTRGSTDASSALNLLVG
                     IRAFVTPYVALFTEYKYSQSTFEFSHAFGPSSGFNGNYDAHILVTGLSYHF"
     gene            39141..39881
                     /locus_tag="PK589_07600"
     CDS             39141..39881
                     /locus_tag="PK589_07600"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNJ19504.1"
                     /translation="MMPTSWLPACHTIFKKRYWATAALFLSAWLLSGCAQSAHQSGRD
                     LLDQILPDQLQQQIDPTVTFPELKANPSAYQGKTIMVSGIVVKSKRRKDQTEIEVLQI
                     PTGTGAVPSKDRAHSQGRFLAVKSGEFLDPAVVDAGTPVTVVGEVGPPVTRPMDDGEY
                     TYPVLAITHLVDWDEVNPAMRSAPYAYPYGRSMYAYPYAYWGPGGWYGGYPGYGYGYP
                     YYGFYGFGGGSSAPAGPPPADAPPQFNK"
     gene            39915..42530
                     /gene="ligD"
                     /locus_tag="PK589_07605"
     CDS             39915..42530
                     /gene="ligD"
                     /locus_tag="PK589_07605"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HNJ19505.1"
                     /translation="MSLRQYWKKRNFRNTPEPRGKETPAGARLQFVIQQHAASRLHYD
                     FRLELDGTLKSWAVPKGPSLDPAQKRLAVHVEDHPLDYASFEGIIPEKQYGAGTVLLW
                     DRGYWEPIGDPKADYRRGRLKFTLHGVKLQGIWNLVRMGGREEAGKENWLLIKEKDAE
                     AKRGTQSRVTDRLTSSVASGRSMEQIATGEHAVWHSNRSSTTSAKKRAVSRPVEKTPA
                     AGARKAAQEEWIVPQLATLVTASPEGEDWVHELKYDGYRILCRLKDGQVRLLTRNGHD
                     WTAKLPHIAAALTDLPAQAAWFDGEVVALSPDGRISFQLLQNAFDAHSDSDLLYYVFD
                     LLYLNGYDLREVPLLERKRLLSALLKDRPSSSLIQYSDHITGRGDVAFAEACRSGMEG
                     LIAKRADAPYLSGRNRNWVKVKCGQRQEFVIGGFTDPSGSRSAFGALLLGVYDAQGRL
                     QFSGRTGTGFTERSLKELHTRLKKIEQKQPAFVNPPVGSEARGVHWVKPLLVAEVAFA
                     EWTNEGQLRQASFQGVREDKDARSIIHERPDSGPSSPKARKGRGHARSRRISSNQQTP
                     SAGAEADETAPVIGGVTLSHPDRVLFPEQGITKIALARYYESISDWILPHVEGRPLTL
                     VRCPEGHKKECFYQKHAGEHIPDTVGRVEIPEEKGSSWYMVADDLPALIGLVQMGILE
                     LHTWGAKRDQLERPDRMILDLDPDPAVEWPLVIEGAQLLRTLLEELDLVSFVKTTGGK
                     GLHVVVPLQRAHTWDEVKTFSKAVADHLVRAIPERFLANMSKQKRKGKIYVDYLRNAR
                     GATAVAAYSTRARPRAPVSVPLAWNELSTDLRSDHFTVDNIAERLEHLRQDPWKTYFT
                     TRQRITKNMVRRLAG"
     gene            42678..42947
                     /locus_tag="PK589_07610"
     CDS             42678..42947
                     /locus_tag="PK589_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19506.1"
                     /translation="MNATTTRTLPLTGKTRSTPAVSQHDTCRCLRCGGFLVDERCMDI
                     GESLGGYWFMAMRCVQCGDITDEVILRNRSAQLATTTQHEVVRAA"
     gene            complement(43037..43408)
                     /locus_tag="PK589_07615"
     CDS             complement(43037..43408)
                     /locus_tag="PK589_07615"
                     /inference="COORDINATES: protein motif:HMM:NF018767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HNJ19507.1"
                     /translation="MEPIGRRRWVIAEGYIPSKSHGPEPQMTSHETMCILNSSDQDAT
                     VQVTIFYADREPVGPYIIQVPARRTKHVRFNDLTDPAPIPLDTDFSSLIDSNVRIVVQ
                     HTRLDSRQAENALFSTMAFPV"
     gene            complement(43446..45197)
                     /locus_tag="PK589_07620"
     CDS             complement(43446..45197)
                     /locus_tag="PK589_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNJ19508.1"
                     /translation="MATTVADLLVERLIQWGVDTIFSLPGDGINGIYEALRTRQDKIR
                     VVLVRHEESAAFAACGYAKFTRRLGVCLATSGPGGIHLLNGLYDAKCDGQPVLAITGH
                     TYHDLIGTHYQQDVDLERLCSDVAAYSQRVMSPAHVGNVVDEAIKTAISRRTVAHITI
                     PKDIQDWSVEDRGSKANIPGHSGDLYSDPLPLPSRSLLEKAATLINAGSKIAILAGRG
                     CLGARAEVLQLAELLGAPIVKPLLGKAVVPDDHPLTTGGIGLLGTAPSQEVLEACDTL
                     IIAGTSFPYLEFYPKPGQARAVQIDLDAARIGLRYPIEVGLVGHCWDVLRALLPLLEK
                     KSDRSFLNTAQASMQQWNELMEERGTRTDTPLKPQVVVRGLNEFLADDAIICCDTGTV
                     TTWAARHIIIRENMEFSASGTLASMGNGLPYSLGAGIAYPGRQIVCLAGDGGCTMLMG
                     ELATLVRYGLPVKIIVLKNNLLGMIKWEQLAFEGNPQYGVDLQPIDFALFAKACGATG
                     LTVDDPGDVREVLREAFAVQGPAVVQAVIDPLEPPLPGKITTTQAWQFAKALAKGQDD
                     RWGIIKTLVESKIREVV"
     gene            complement(45216..46397)
                     /locus_tag="PK589_07625"
     CDS             complement(45216..46397)
                     /locus_tag="PK589_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNJ19509.1"
                     /translation="MKPGYVETLAEERPRVQTSKERGTSVANRHLWEITPVRDLLWLG
                     GILFLLWFGYYLRGVFTPVLVALLLAYLVNPLVRRAETAWHIPRPVTVSIVLLLSAMM
                     LGGLVTWLGPLLAEQVQSFAERVPGYLQSIATQYHVTLGNFSEPLSSIATSLRDDPLS
                     ILKPIFSGTGQAFGVLGSVIGTTADVTLALVLIPIYFFFFAWQFDSALAQLRRYIPPG
                     YRPRARHIVRRMDQAVSGFFRGRLTIALASAVLYSVGWALTGIRYWFILGLITGILTI
                     IPYASLIGWPLAVLLKYLDGFSAGAGSFDVMTVVVWPSVAYLVVQFIESWLLTPWVQS
                     QSMEMNAVTVLIVVFIGGALGGFYGLLLAIPIAACLKILVQELVLPRLEHNAASPPPS
                     F"
     gene            complement(46440..47354)
                     /locus_tag="PK589_07630"
     CDS             complement(46440..47354)
                     /locus_tag="PK589_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNJ19510.1"
                     /translation="MSASLTSASPRWNPWKLGGLGWKELGQRLWTDSQKDEILGRAAQ
                     LAYYFLLALFPALLFLTALLGLFPLEQVLPELMTYLRTVLPADALSLLQKYLDSVVKG
                     SGSDILSLGLLGALWASSSGVTAIMESLNVVYGAQETRPYWKVRLVAALLTIGLAAFI
                     IISITLVLYGERIGEWIADLIGLGWLFLLTWNVLQWPVAIGLMLLALAIIYAVCPDVD
                     HDWRWVTPGSVVAVLLWMGLSLGFKAYVDHFGNYNAAYGSIAGVIVLMLWLYLTGVVI
                     LLGGEINAQIERAALALRSFSEQSSHTA"
     gene            complement(47370..48029)
                     /locus_tag="PK589_07635"
     CDS             complement(47370..48029)
                     /locus_tag="PK589_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19511.1"
                     /translation="MDIAEKLELLEQRIKDTAEGATMKFSRLLDEGSQSVTQMVDKTK
                     AALDPIHKVDDYPWLMLGGAICAGYAIGLLEARTRAQRGVYPYYPPGAHASQVMPESE
                     TEGKTASRRAEGIYDYYPDPTHRARRTEQRSHSTIWETMSRELEIDTEQAKAALMQAG
                     RTLMVEFANKLIPEIARSLGVTLTSQGKQSRPSYDQASYSETEGRGNARSSAGSASQF
                     R"
     gene            complement(48050..48457)
                     /locus_tag="PK589_07640"
     CDS             complement(48050..48457)
                     /locus_tag="PK589_07640"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNJ19512.1"
                     /translation="MENPHPISIASLVTGLVEDLRRLFAQEVQLARHEMQHELGKVVK
                     SAMHAGIAMMLAFLAVLFLLMTLIHLLHFYSGLPLWACYGIVALVAAAAGAAFFARLI
                     AIGTTLRLWPFRTFYSLKEDAKWIKEQLLSTKT"
     gene            complement(48476..48826)
                     /locus_tag="PK589_07645"
     CDS             complement(48476..48826)
                     /locus_tag="PK589_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19513.1"
                     /translation="MEQDMKHMADRAKQAGSSIQDAIHDTATSAEHMAKRAGDAVESA
                     ADHLRDRLPKDSAAGEAADTVVRKVKETSVYLQEQGIGGVVEDLEILIRRYPLQTLLL
                     GLGCGYLLSRARTD"
     gene            complement(48960..50063)
                     /locus_tag="PK589_07650"
     CDS             complement(48960..50063)
                     /locus_tag="PK589_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNJ19514.1"
                     /translation="MSHSPLRVAVVGLGHISQMAVLPAFSHTGSAATLTALVSDDPVK
                     RRVLAKRYGVTHTYPYKDFDACLRSGEIDAIYIALPNSLHCEYAVRAAKAGIHILCEK
                     PMAVTAAECRRMIQAAERHRVKLMIAYRLHFEACNLFTIDLVQSGRLGDPRLFHSVFT
                     QQVRAGDIRLRQQLGGGPLYDIGVYCINAARYLFRDEPTEVTALAGRGPDHRFQSVEE
                     SAGAIMRFSRDRLATFTCSFGAADAATYEVVGTKGKVRVEPAYEYVGGLKASVTIGGR
                     TSVRTFTPGDQFAPQLIYFARCVTHDRQPEPNGTEGLLDVQIIEALHRSAQRGQSITL
                     RLPAKRLRPDLTLRMHRPPVSKPQGVHTESPTL"
     gene            complement(50143..50559)
                     /locus_tag="PK589_07655"
     CDS             complement(50143..50559)
                     /locus_tag="PK589_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNJ19515.1"
                     /translation="MEQRPTLHETADIIKIMKNDHRHILALFQVYLGTESDSRQSIVD
                     DILQRLDDHFDWEERLFEEDSRLQEHATPVIRRVLLDHEEVKAMIHELRHAETDDDES
                     MDQFFEDMMQTVRVHFHGEERDLIPLLDAMTTAPRG"
     gene            complement(50598..52067)
                     /locus_tag="PK589_07660"
     CDS             complement(50598..52067)
                     /locus_tag="PK589_07660"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNJ19516.1"
                     /translation="MTRHVSHILPVLFSALLVGACSHSIPKSPSKPVPPPLTSAVPTF
                     PAQTKEDSRAPVSQVPVQIKTDLTPIQTAIRKAIPERITETGHPLGQEFRWTFVRVGE
                     PDVHIQDGLVAIHAEYKGDIESRGSSRACRLDPLYTSIDATGRLTMIQNGDALSFGFE
                     PAQTAVTTKPDSDARCNMFNVPISEQLPDLFGMSEIKTALAEAVHPKEFGIPLQRIWD
                     DLGGPLSIPIASLNTRACMYGNPREMILGGQKGTTQETVISGTAKEMPFITYEATCTE
                     ATPTVALVNSGSIPPEGKPYTLVARIPLSYQQISHQLQSKLFHQSVFLDTASDTAVIE
                     RVSASDANGRALVAIDMSGDLKGTIYYWGTPRLEEGGRSISVPDLQMANESKAAIDSI
                     RIGYWQLVDRELNGKLRQAMTVDISPQVDRLKQTVTGTHRSGGTTMDILITRQQPDHV
                     RSSAQGITATIMLEGTANATGQVTLEEQSPRALLRQDVR"
     gene            complement(52144..52452)
                     /locus_tag="PK589_07665"
     CDS             complement(52144..52452)
                     /locus_tag="PK589_07665"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNJ19517.1"
                     /translation="MMKLLTHLMFAFAMMTLVACQSTTGKTAGQTIDDASITTAVQAK
                     LSQDRLSNFSRIDVDTERGVVTLNGVVKSAEQKMRVVELARGVAGVRTVNNNLQIQAP
                     "
     gene            complement(52513..52839)
                     /locus_tag="PK589_07670"
     CDS             complement(52513..52839)
                     /locus_tag="PK589_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNJ19518.1"
                     /translation="MHTDDSSNWSAFMAGALIGAGIALLLAPQSGNELRSTLRDYASR
                     AKDELDEATEQGRAAWDSAMERGQEYVEGGKQTLRKAGRAAREFVDETMQTAQNTAEE
                     ATSRRG"
BASE COUNT        10959 a        14841 c        16292 g        11038 t
ORIGIN      
        1 aggatgtcgc agaagcgctg gttgacgaag acgaagcggc cggtgagatc ggtctgtgcg
       61 accgaagccg tggcctggct gacgatggcg cggtagcgcg cctcgctggc ggccatcagt
      121 tcttccgccc gtatctggtc cgtggtgtct ttcgcgatgc cgatgatctg cctggctcgc
      181 ccctccggac tgcggtcgaa caccgaggcg cgcatgagaa tccatcgata ggtcccgtcg
      241 gcatgcctga gccggtgttt gtgttccaac acctcggcat cggctgccga gtcaaagcgg
      301 cccagccatt gctcgacaga cggcacatca tcggggtgga cgagggtgac aatcggattg
      361 tcctgcgtcg ccgccagctg ttcgacgtca tagccgagca cagatttcag actgtggttg
      421 atgtagacgg tgcgagtccg caggaggtcg aacacgaaca ggagatccgg tgtggacgcc
      481 gcaatccgct ccaggaagag tttgctgcga gtgatttcct gctcggcctt gcggcgaatg
      541 cgcgagagat ccaggtgggt cgtcacgcgc gccatcaatt cctgtgcgct gaatggtttg
      601 acgagatagt catccgcacc ttgttcgagc ccctcgacac gggattcctc gcccgccctg
      661 gcggagagca ggatcacggg aagggtatgc acggcgggat cagcgcgcaa cgccttgagc
      721 agggcaatcc cgtcgagctg cggcatcatg atgtcactca agaggaggtc gggcgggtgg
      781 cggcgaattt cgttcaacgc tgccagccca tcaccaaccg tgacgacgtc gtaccgtctg
      841 ctcaacaagc gctcgatgta ttgccgcatg tcagcattgt cgtcggcgac cagcacgcgc
      901 ggacggatga ctgcgctcgc ggcctcgctg gtcgaagccg gcggtgggat tgcttgcgta
      961 tcaggcagcc aatgcagcgc ttcctgaaca taggcaacgg cgctcgctga ccggactgac
     1021 ggctcatcac ctgcgcggat ctgttcggca ggcaggtggg cggttcccca cgagatcctc
     1081 accgtaaagg tgctgccttc atccagacgg ctctcgactt gtaccgagcc tccatgtaag
     1141 cggaccactt cctgtaccag tgccagaccg atgccgctgc cttcatgggt ccggctgcgc
     1201 cggttctgca cgcggaagaa tcgctcgaag aggcggggca gtgcctcgtc cgggatgccg
     1261 actcccgtgt cgctgacgac caattcggcg cccgccgcgt cgtgccgcat cgagatggtg
     1321 atctgtccct ggaaggtgtg cttgaaggca ttgctgagca aattgagaac gattttttcc
     1381 cacatgctcc gatccacgta gacggcttcc ggcagtggcg ggcaatcgac aacgaacgtg
     1441 acccctgctt tttcaatggc ggagcggaag ccgcttgcca ggtctgcgct gtaggccgcg
     1501 aggtcggtcg gttgataact ggcctcggct cgccccgcct cgatgcgcga aaagtccaat
     1561 aaggtgttca ccaatttgag gagccgcagg ctattgcgat gggcgacttc gagggcagga
     1621 ctctgtggtc ggagatgcag ctcttcctcg agcggcccga gcatgagggt gagcggcgtg
     1681 cggagttcat ggctgacgtt gctgaagaag gcggttttcg cccgatccaa ttcagccaag
     1741 gcctcggccc ggcgctgctc ggattgataa gccctggcat tgttgatggc gacggcgata
     1801 tgcgagctca ccatccggaa gaaatggtga taatgctcgt ccagaggccg acggggactg
     1861 atgcctgcga ccaggacgac accgccgtct tgggttgccg tgccaatggg cagcaggagg
     1921 cattgcctcg tcggttcagg ccatgcgccg ccaggcaaca ggccgaaccc gtccggaagc
     1981 tcgtccaaaa gcaacgggcc tccattacga atggcggcgg caagaggcca acttcccggc
     2041 gccgcattgc cggtcaggtc cagttgttct tgcctggcca ggctggcatc ctccagccct
     2101 atgctgccgg ctaactgcgc ctctcgatga tcccgatcga tctggtagag cagggcgaag
     2161 ggcacatcgg cggagtccgt ggcgagggct tggagaatcc tggcgcaggc gtcggtgtcg
     2221 ttttgcgctg atcccgacag cgatgccagc tcacgcagca gactcgttcg ccggtcgttc
     2281 agcacccggt acgtcgtttc ctggacgata ttcagaatgc cttcaacggc tccgctttgt
     2341 ccgcgaatcg ggttgaggct gtagtcgaaa taacattcct ccgtgtagcc gaaccgttgc
     2401 atcggcagca gctcatcagt ccgccaattc gcttctcctg tgttgagcac gctatgaaag
     2461 taatgctgga tggtcggcca caattccggc cagacttcgc gggcagggtg gcccagcgcc
     2521 cagggatgtt ttccgccgac aacagggcgc caggcgtcgt tgtacaggag ccagctatcg
     2581 gctccccagt agatcgccat gggggtttgg gctgtcaggc agacgccaag gatcgatacg
     2641 agggaggcgg gccactgttc gatggggcct agcggcgtcc ggctccagtc gaatgcccga
     2701 atgcggccac ccatctcgct gccggtcagc cagctgaaca tatcggtcgc cttcattccc
     2761 ccttccaaag ttgtctgctg gttgatgagg gtcggccgat catacagggg cagagattga
     2821 ttctctgccc ggggtcactc ctcttgaatg aaggacaccg ctgatgcgga gcggaacctg
     2881 gcgacaggcg tccgtccgag ggctgcacct agcgttattt ctgtagcatg aatgagacgg
     2941 gagcccccac tagtgaaacc cctagtcctc tgggatgagt tgagagactt atcgggcggg
     3001 agaaggagag ggaagctccg gtgcggccgt ttccatgacg cccacggctt gttggccgga
     3061 atccagcttg atggtgaccc gctggccgac tgaaaggtga acaaacagtt caggttttgg
     3121 aatgcgaaac gggacttccc ggccgagatc tgttcctaat cggccctctt ttttctcgag
     3181 atcgaggtgc agcaatttcc cagaaacggc ttcctgtgaa cgaggtgccg cttcggcaat
     3241 caggaagaga gaggtccgct cgactcccga ccatgcctgt agcaacagct tcggtaccgt
     3301 cggaggcgtg ggaatcgggt cgctgggttt cggtggcacc gtgggtcccg gcccgggatt
     3361 agggacaggc gcagggacgt cttgttgaac cgcctgccct cctgtcagca ggacgactgt
     3421 gagcagaccg cttcccatca aggtattcag catcatattc tccgttcgcc ggggccagct
     3481 aggcggttcc gccggtcgat gcaggcacgg attccaacac cattttgtga cagagttccg
     3541 cacaccgccg gcattctttg gcgcagtctc cgcacatggc atggtgtggc gcatgttgct
     3601 cgcagacgcc ggcacagagt tcacacattt cgacacagaa cgcgcaaagg cgaacggcaa
     3661 aggccgaggt ccgtcccatc cactgcgccg caaggaggca gatgtccgcg caatcgcggc
     3721 aaagacggat gcaacgcacc atgaggtcct gggcctcttc gtcctccgac atgtccatgc
     3781 cgatcatatc gttaccacag gtgttgcaga tacgcgcgca atccaaacaa gcctgtatgc
     3841 aatcaagtcg atgctgatct actgattgaa tcgatgtgga catcctcatc gccctccctc
     3901 ctctttgtgc cagtgttcag cctatcctac tgagatagtg gcgagtacta gggaaaactc
     3961 tcgaaccgtt gaaggatggg cgcggaccta ggggtctgac tagctgggta tcgtgaagaa
     4021 agttgggtag ggtgtgcggc aagatggcga acatcgcgat gcacaacgca gcggaagggg
     4081 gggcgctgtc gtctcctcca acagagccgg aaggctcttc gcccatactc gtccctggac
     4141 acacctgttg gcgcatagag ccagcggaac gaatcgcgtt cctcgttgac ggggaggcgt
     4201 acttccaggc gttccgtgag gcggcgttgc gcgcgaaaca ctccatgatg attgtcgggt
     4261 gggatatcga tacccaggtc gagctggtac gcgaaacctc tgcctcgtcg gagatgccga
     4321 ccaagctcgg cgagtttctg gtggcgctgt tacgccgcag acgcaggctt cgggtgtctg
     4381 ttcttaactg ggattatgcg gtgatttatg cgctggagcg gcaatggatg cccacggccg
     4441 aaccggggtg gcgccggcat cgacgtctct cgtaccaggt cgatgaccac catccggtgg
     4501 gcgcgtcgca ccatcaaaaa ctcgtggtga tcgatgatgc ggtcgccttt gtcggcgggc
     4561 ttgatttgag taagtcccgc tgggatacgc gggagcatct ttcccgggat cctcgtcgtg
     4621 tcgacgccga agggatagtc tatgccccgt ttcatgacgt gcaaatgatg gtcagcggcg
     4681 acgcggcgtc ggctttgggg gacctcgcgc gcaaccggtg gttcaacgcg accggacggc
     4741 gactttcagc gccgccccca cgatccgtcg aagaggtgtg gccgccgagg gtgaaccccg
     4801 atctgacgga atgcccggtc gccatcatgc gcacccagcc tcgatacgaa gagctggaag
     4861 agcaacgcga gatcgaacag gcgtatctga gtgccatcaa acgcgcgcgt cgatcgattt
     4921 acatcgagtc gcaatatttc accgctcaag ccattagtca tgcgctcgcc caacggctgt
     4981 ccgagcatga cgggccggat gtggtgttgg tgctccgtca gcattgcgac gggtggctgg
     5041 agcgccggac gatggacagt ttgcgcgccc aactcctcca cgaagtggaa ctggctgacc
     5101 actatggccg gctgcgtgtg tatgcgccgg tcgtgccggg agaggcgggc gccgaaccgg
     5161 ttgccgtgca tagcaaactt ctggttgtcg atgacgaatt cgtctgcatc ggatccgcca
     5221 atgtctccaa tcgttccatg ggattcgaca cggagtgcaa cctggcagtc gaggcgtgcg
     5281 gccaggcacg agtgcaatcc gccattgcgg cgtttcgcaa cggattgctg gcggaacatc
     5341 ttggcgtcga tcccgaagtg gtcgccaatc agattcaaac ctgcgggtcg ctcgcggcgg
     5401 cgactgacgc attgcgcggg ggcggccgga ccctggccga cgactgtttt gaagcgatgc
     5461 ctccttccga tgcaatgatt cctgaacatg cgctggtcga tccggagcgt cccatggccg
     5521 cggatcatct tctggccggc gtgggattcc ggcgggagcg gtcgcggatc gtccggcgcg
     5581 ccatggcggg cctcacgttg ctggcaggaa tggcgatact cgcagccgcc tggcgatgga
     5641 cggctctggg acaattgctg gatgttgaag cgttggtgag ttatgcgcag gcgctgggac
     5701 gaagcccgct ggggctcata tctgtcatgg gcggatatct cattggcggt ctcctcgcgg
     5761 tccctgtgac cctgctgctg gtggtgacga tcctcgcctt tggtccctgg ttcggtgcct
     5821 tgttcgcgct gaccggatcg ttgttgagtg cgatcacgct gtttggactc ggccgggtcc
     5881 tgggctcata tcggatccgg cgattggctg gttcccgcgt ggaacacctc agtcgacgta
     5941 tggccgaacg agggctttgg gccgtgctcc tggtgagaat cctcccgatc gcaccgttct
     6001 cggtcatcaa tctgattgcc ggtgcctcat cggtgtcatg gcgacacttt gtgctgggca
     6061 ccgttgtggg catgttgccg ggaatcgcca tcatggcgac cttcgtgagc ggtctcgaag
     6121 aagccgtcag ggaaccgagt tggcaggcct tctcgatggt ggggttggtc gtcgccgcgg
     6181 cctgggctgg cgtgtggtac gtatcgagcc gactgcagtc caggcagtcg actgagagtg
     6241 tcatggccgc cggttcctcg gttccagcga cgtaggtcct tcagcaaagg ggtgccatcg
     6301 atgcgtgtgc aggtggcctc gtacaatatt caccgcggag tcggaaggga cggacgctat
     6361 aatcccgggc gaattctgcg cgtgctacag gaggtgaacg cggatattgt cgcgctccag
     6421 gaagtggatt ttcccggccc gcatccgcat cactttttga cgtggctggc tgctcagcta
     6481 ggtatgacgg ccgtgggtgg accggtgcga acatcggcgg caggcaccta cggcaacgcg
     6541 ttgttgacgc ggtatccggt gagaaacgtc aggcgatggg atctttcagt cggaagacat
     6601 gagccacgag gcgccctggc tgcggacatg ctctgtcagg gcgcgatgat tcacgtagtg
     6661 acgacacact tgggactgtg gccgacggag cggcctcggc aggccaccaa attgctggag
     6721 ggtttacacg tcacagaaga agagttgacg attctcatgg gcgacctgaa cgaatggcaa
     6781 ttgtggggga agtcgctgcg ggtgctccgc aaggtgttcg gggcgcccgt ctcgccaccg
     6841 acctttccca cggcctggcc tctgttggcg ttggatcgga tttgggtctc tccgcccagg
     6901 gcattattcg aggtccacac ccatcagtcg cgccagagtc gcaccgcctc cgaccacctt
     6961 ccggtgaagg cggagatccg gtttgagtcc gtgcatggca cagggtctgt gatgcgggag
     7021 gactacgaac cgacgcctac ttcaacaggc tgtggccgtt caatgctgta gcggccatat
     7081 cccaggtggc tcaagcgtag ctgtcccata cggctcaacc ggtcgacctc tgaaaatacc
     7141 tggttccagg tgagagtcgg gcaggcttcc accagcatgt ccatatcgca ctgctgcatg
     7201 tggtcaaacg catcgaggat ggtgggggtc acgctgttgt cgatttcagg ctgctcttcg
     7261 gtcactgcag ggcgagcagt tctccccaac ttggtaaatt gcacggcgca tgcggggcag
     7321 taggtgtggg tgagacgata ccctccgatg gtcaggtgat gcgtatcgag atacgtcttt
     7381 agagggcccc actcgggctc ggtcccgggt gacagagtct cgcctcgcac attttcacat
     7441 acgcagcaga tggggataaa cttcttcatc tctgtcttca taacccaccc ctctcttctn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gcagatgggg ataaacttct
     7621 tcatctctgt cttcataacc cacccctctc ttcttgtcgg aatgaactgc aaccggtcaa
     7681 ctagtgtcgc actgtagaaa atattctgcc ctccgactac taggtgctcc cctagttctc
     7741 gcccgtcatg atgtggaggg gcgaggagtg ggcctggctc aacgctcaag ctgtacttta
     7801 ccttcgaggc cgggcaggac cgcgtcgaag aaggcgtttt ggaagagggt cagcaccagt
     7861 tcccaggtgc tcgcttgggg gttttccacc gttccggaca ggtcagtttt cgtggcgact
     7921 tcatcccgcg gggtgttttt gagaatttca gctaacgcat tgatggtctt ttcgtacagc
     7981 tggttgagca tccccttatc ccggtcctgt tgcggatcat aggccgtcac atccttaaac
     8041 aggggcttta agtatccctt gacggtgccg tttttgacgt tcacctcgct gaacagcgaa
     8101 aacaggccgg ccgcggcatc cagacccccg taggcgcgca acaattgatt gagagatttc
     8161 acctgcgttt tgacgatctt cacagaaagg tcaaagtccg gggattcgac ttccggcctg
     8221 aacgccccgg taatcttggt cgcgccggtc cccatcaaca tgcccttgag ttgcacccgc
     8281 gccgtgcctt cggagagctg gttgctccag ttctcaagct ccagctccgt cccgctgatg
     8341 aacaggcgat aggaaggatt cgtcgcctgg tttacaaacc cgaactcact gcgttcgact
     8401 ttcccctggt ctatccgaag gaggagcgtc ggatggttgg cagctttcgc cgccgtccgg
     8461 ctcacctgtt gtgccgtacg ttgctcgcgc tgtttcgtcc gggctgcatg aacaaagtcc
     8521 accttcacat catgaaggtt cagcgaagcg atccgcactt cttgcgccgc aggggcaaat
     8581 tccacatgac cgctagtcgt caatgaacct tgggtgaggt ggatttggcg ttgagcggtg
     8641 agggggagca ggtcggccag gttgatgtcc tgcagcgtga tgtccacatc gacgcccatc
     8701 gacggttcgg caaaaaagtc cgcatgccca tcgactttca tgtggcctcc accggccacc
     8761 atcgcttcga cctgcacccg cgaggggtac tgtaacggtt tcgacctcac gtttcgaatg
     8821 ttctccgcca ggacctgaat gttcgtcagg cgtatgggat tggacgtcgc gttctctcga
     8881 taggtcacgt cgccgtcctt gatactgagt tcattgattt gtagcgggta gacggcgaag
     8941 agggcctcct gccaactttt cttttgagcc ggcgcgtcct gcagttcttg agccgcttgg
     9001 ggtcgggtga agtgaatgag aggacggtca atgatgtgat cgctgaccag gcggccggat
     9061 agcagcccct cccactggat gctggcatgc cattccgcga tgtgtgcgag tggtggatcc
     9121 ggatgggcct cctggataac ggttacgttt tccaagtcca cagacaggcc aataggatga
     9181 aagtcgagcg taccgatatg gaaggtgtag cccgcgatac ggccgttcaa ctgacgttcg
     9241 gcatagaccc gcaagggttc atcgacaaac cataacccta ttccacccag gagaatcacg
     9301 gccgcacagg cagccatgac ccagcgccac atgccgcgat ggtgtttctt gacagacgat
     9361 gcggcatgca gactctcacc cgtgtaggtc gtctgtcggc tatcgatccg aatgctcata
     9421 accgtctgac ccgctgaata acgacaccta cctcaagaaa cccccgacct gtcggcggaa
     9481 taacacgaca tggacattgc gctcggatcg taatgccgct ttgcctgctc ggctactggg
     9541 aaggcccctg gtttcgagaa ggcgtgcagc cgagaatcgg cccttttcgg ctctagggta
     9601 atgccgagtt atgcgcggca gacggagcgt gacagtgtaa ctgcggacgt aagcaggtga
     9661 ggatcgctct acttctcgtc agccgaattg aagcgtcagg tccttacgat tggcatctcg
     9721 ctagaccctg ccattacgaa cgatcatttc acgtcgagag gggcccgcaa gatggcacac
     9781 acatggaaga cgctgctgca accgcaatcc ttgtggaaac tgcttcagga agctttcacg
     9841 gcctggagtg atgacaagat tccgcggcac ggggccgcgt tggcctacta taccgtgttg
     9901 tcgctggtgc ctctcctgat cgtgatgatc tccctcatcg gactgatctt cgggcgggaa
     9961 gcggcgcaga aacacatttt ggatcaaacc accaatctga tggggccgca aagcggggac
    10021 gcggtcaagc aaatgatcga gcatgccacg cagcccacca cgggaacgat cgccatgctg
    10081 atcggcgtag ccacgctgct gttgggcgcg tcaggcgtat tcgcgcaact ccaggactcg
    10141 ctgaatacga tctggagagt agagccgaaa gaggggcgcg gcttgtgggg actcattcgc
    10201 gatcgattta tctcgatcag cgcggtgctg ggtacagggt tcctgctgct cgtgtctctg
    10261 gttctcagtg cggcattggc ggccttcggg aaatggtata gcgggtggct tccggcgccc
    10321 gaggtggtgc tccaactctt ggaaatcgtg atttcgttgc tggtgattgc gggactcttt
    10381 gccttgatgt tcaaggtgct tcccgatgcc cgcatcgcgt ggaatgatgt gtgggtagga
    10441 gccctgttga cggcgttcct gtttacgatc ggtaaattcg ccattggcct gtatctggga
    10501 aaaagcgatg tggggtccgc ctatgggtcg gccggatcac tcgtcatcat gttggtctgg
    10561 gtgtactact ccgcgcagat cgtcctgttc ggggcagaat tcacgcaggt ctatgccaat
    10621 gagctcggga gccggatcgt cccgacggag aatgccgtcc cggccgatga gccaagggcc
    10681 ggacgcatgc gcccagcacc gacacagcca agattgcagc cggcatccgg tcggcaagca
    10741 caggctgcct cagctcagcg tcagcctgca gagcgcctcc cttggtgggg aacagtcctg
    10801 ctgttatggg tggcagtaac ccgcttccca cggcgttcgg aatgaacaat gaacagggac
    10861 cgagatggaa tggaggagcg gccagaaccg cgtgaacgat tgaggtgaac ggttgagagg
    10921 gcaggcatca aacgacgata tgaagagagg aacaacacgt atggctggaa agcaggatat
    10981 gaagacgaat caggggcatg gtggagaaac gcatcagcag gcagggggac ccaaagagcc
    11041 ggtgttgacg accgaccagg gcattcccat ttccgacaat caaaacagcc tcaagagcgg
    11101 tgcgcgaggc cccacgctgc tggaagattt cgtgctgcgg gaaaaaatct tccatttcga
    11161 tcatgagcgg ataccggagc gcattgtgca cgcgcgggga tcggccgcgc atggattctt
    11221 tgaagtgtac gaatcgcttt catcattgac gagcgccgat ctgtttcaac gcaaaggcga
    11281 acggacgcct gtcttcgtgc ggttttcaac cgtcgtcggt ggcgccggct ccgtcgatac
    11341 tccccgtgat gtgcgcggtt ttgccgtcaa gttctacacc aagacgggga attgggacct
    11401 ggtcggcaat aatattcctg tcttttttat acaagatgcc atgaagtttc ccgacctcgt
    11461 ccacgccgtc aagatggagg cagacagcgg tttcccgcag gcggccagcg cgcacgatac
    11521 cttttgggat tgggcgtcgc tcatgccgga aagcacgcat atgttgatgt gggccatgtc
    11581 cgaccggact cttccgcgat cgtttcgtat gatggagggc tttggcgttc atactttcaa
    11641 gctggtcaat gacaaaggca aagagacgtt cgtgaagttt cactggaaac cgaaactcgg
    11701 cctgcaatcg acagtctggg aagaaacgct gaagttgcag gcggccgata acgattacca
    11761 ccggcgtgat ttgcgggagg ccatcgatgc ggggaatttt ccggagtggg aattgggcat
    11821 ccagacgttc gatcaggcgt ttgcggataa acagccgtat gacgtcctcg atgcgaccaa
    11881 actgattccg gaagaacaga ttcctgtccg acgcatcggg cgcctggtgc tggaccgcaa
    11941 tcctgacaat cacttcgcgg aaaacgaaca ggtcgccttt tgtcccgcca acatcgtgcc
    12001 cggcattgat ttttcgaacg atccgctgct gcaaggacgg ttgttttcgt atctcgatac
    12061 gcagaagtcc cggttgggga ccgccaattt tcaccaactg ccgatcaacg cgccgaagtg
    12121 tccaatggcc aactttcagc gcgacggcca gatgcagatg cgagtgccga ccggccgggc
    12181 caactacgag ccgaatagtc tggctgccca tggggaggaa ggtggtccca gggaaagccc
    12241 gcagggattc accaccgcac cgggccgtga cgatcgggag gaacagggcg ataaactccg
    12301 cgttcgtccc gaactctttg ccgatcatta cagtcaggcc cgcatgttct ggaaatccct
    12361 cacggacaat gagcaggccc atctcgcatc agcgtttgtc ttcgagctgg caaaggtcaa
    12421 cctcgacgag gttcagtcgc gtatggtcgg caggctgcgg aacgtggatg aggggctggc
    12481 gaagcggatc gcggatggtc ttggcatgcc gctccctggg aagattcgcg cggccaggga
    12541 gccgatcgag atgaagccga gccctgctct gtcgatccag aaaaatatgc tggcgacgtt
    12601 gaagggacgc aaggtcggcc tgctctatgc ggaaggatcg aacggcgggg acatcgatcg
    12661 aatgatcaaa caggtcgaga aggccggcgg gaccgtcttt cttgttgcgc ccaagctcca
    12721 cgggacgaaa ctggagaacg gcaaggaatt gaaggccgac gggcaactgg ctgcctcacc
    12781 gtctcagtta ttcgatgctg tggccatcat cctctcggcc aaaggcgcgc agatgttgct
    12841 caaagagggg gcggcgattc aattcgtcat ggatgcgttc gggcatctca aggcgattgg
    12901 ccattcggag gcggcgcagc ctctgttgga caaagccggt gtggtaccgg atgaaggcgt
    12961 gaccgatctg cgagggtcat tcgtggaggc agccgccaag cgcttttttg ctcgcgaacc
    13021 gtccgtgcgt acgttggcgt agcaggcatc ctcagcgttg gagtggaacc acggacaggt
    13081 atggggggag tgaaagcccg ggctggtgtg cgatgcctct atcgacagac atcgcacacc
    13141 agcagccgtt tctgctttac tgaaccgtga tggaaccttt cgtgtcagcc atcgttgttc
    13201 gcgtatcgct cgaaggcctg accgagtagt tcacggtgcc gacccggctg aaacagagtc
    13261 cggccgtttc attgctcgcc aacgtggtgg aattatcgac gcccatccct agcgccttgg
    13321 agaatccgtt gcggcaggac acattgtgct ccagggggcc gtcaattgtg accgtcacgg
    13381 aatcctgtcg ctgattgatc cagcgaacct cgtcgcccgc ccgcaccgtc aaggcatcgg
    13441 gagtgagcgt atctttgatc acgatatttt tcaccatgcc tgttttggtg cctacgtcca
    13501 tttctgaccg ggtcttcgag gcggttggtg aactcgagca ggcggccaac gacaccagcg
    13561 cgagggcaag tagtgagtgc atcaatgtct tcatgggtaa tcctccttgg taagatgaaa
    13621 tgatcgcgac atcaccgcga cgtgccgctg atgcgatagg ccatgtgtcc tctgtgggag
    13681 tgcgaggcct ccatgcccac agtactcagc acgatgacga ctccgtactc gggaaagccc
    13741 cagtatgcgt caggcggccg gatggttatt catgaagaaa gactgaatgg gtgagggggc
    13801 atgagtccat ctgaaatcat tcgaaaaagc ggaggttcag cgttccatcg gctgacgggc
    13861 ctgctactca cgctcgtctt gctcctgagc ggctgtgccc tctccttcgg gtatcggcat
    13921 gctgactggc tcatcacgag gcaactggat cattacttgg acttaacgtc gagccagcgg
    13981 cgcatcgtca acgcccgcct gcagccattg ctctggcggc accgcatcga agcgttaccg
    14041 cagtacgaac agtttctcaa agatcttcac gcccgcatcg gcagggggtt gacctccgaa
    14101 gacgtagaat ggacttttgc ctcctacgat cggtttcggg cagatctgtt tgagcgggcc
    14161 attcccgaag ggacgcagct ggcgatgctc ctgaagaata accagatcac ctatctggaa
    14221 cgggtgtttc gcaaggagga ggttcaagcg gagagtctgg cgcgacgatc agtttctacg
    14281 cgccttgatg aacgtgcgag aaaagggatc gcgacggctg aagaatggct tggacctctg
    14341 agccacgagc aatccgttca gcttcgcgag atgatgttgg ctctgcctga cagtcaaccg
    14401 acgtggtggc agtaccgtcg acagcggcat ggtgaggtgc tggccatgct ccgcacttcc
    14461 acctccgccg atcagattgc ggagcggtta cgatacctgt ttgttcaatc cgatcagacg
    14521 gccccgaccg gttacctcaa gatgatgacg gaaatgcgcg cggcactgac gcgtatggtc
    14581 ctggacgtgg atcgcatgct gacaccggct cagcgacgtc atgtgctggt cgcgattcag
    14641 aaggtgatcg acgaggtaca tgcattagcg cacaattcgt aactagaagt ggaccacccc
    14701 gaaaagccac agcaacagaa tcaaactcag cggcacgcca agcagccata atataatcgg
    14761 catagtctct ctccttcgtc atcacactcg ttgaaatgat cggaagggag gggcaccgaa
    14821 ctgggttcgc tacccctcgc cctccgacag agcggacatt cccggacgga tggaaatgtg
    14881 cgctgctatt gtcacgatgc aatggaacag tgtgatgcgg tactaggtga agcccttggg
    14941 aacgagatgc tgggctgcgg gtattacgca ggttcgatcc ctgtggcagg taccgtgtac
    15001 tgttcttcaa tggccgcgcg aatgagccgc cggctttcct cggctgagag ctccggtcgg
    15061 gcttcaatgt cgtggaccac cttggagagg gtttcatcct tgatcatgtc gctgatccac
    15121 gtggaatagt catgggcgtt aagatggtac tgccaggttt gttcatcgac gccctcgccg
    15181 agctgcataa agagcaagag gttttgcgcc cgcaaattca atttccccgc cggacctcta
    15241 aaataaaaac tgcggtccgg cggaagttcg ccttccgcat acttgcgtct atgtctccgg
    15301 cgttcagtgc tgctgggggc aatttcgagg acttcaggcg cgactgctaa cgttggatcc
    15361 cacatgagag tctgaccggc ttcgagttct gttgagggca aggttggagc ttgctgtcct
    15421 cttgcttggc aaaaggcctc gatggtcttc cccggtgatt gcccgagggc gaacaccaga
    15481 tccactgttt tgagaatggc cgggtcaatc tgatcggggt gtacggtcac atacatcatg
    15541 cccgtgcagt cttgcgtcag gcccggggcg ccgggattgc cgtcgcgcgg gagaagatgg
    15601 tgcgtttcat cgatcaagat ccaatgcggg cgaccgcttg ccgcgcgcaa ttccagaatc
    15661 ttgtggagca gaccgaagaa aaacgccggc cggtcatgta acggaagccc gacgaggttc
    15721 accacgacat gtgtctgcgg gtttgcgaga gctgtcagga tttcggtgag gctgggacca
    15781 ttctgggcgg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngacagctcc
    15901 agacgggcca gcgccgccgt caacccggtg gctttgttga tgccatgcgg cagcaccatg
    15961 acggcgccct tattgaaaat gatgcgaagt tccagtccaa gatcgcggat gatgtcgagg
    16021 atcacggttt cgtaggggac acgggtggcg acaatgacat gtccgaggct gagatgctgg
    16081 actgcgcgac gttggacctc ttcgagaaat gccggtcgtg gggcgtcggc cagaagagtc
    16141 acctcttttg tggcggggcg gtacaacaca ccgccgttct ctgccaccac taattcgcac
    16201 aacgaaatct gcggaaaaat gtccagcagc tccggcagga ttcgacccgt gacgagaatg
    16261 agtttgcggc cggaagcgac gagccgttcg agggcggcga cggtttcggg atatacggct
    16321 ccgtctgacg ccagggtgcc gtcgtaatct gctgccaagg ccatataacg catcggttcg
    16381 tcctttcccc aatcatttgc cgtgacccag tgcgatgtat acaagcttcc aacagtcggt
    16441 ggagctagga aggagcctag ggagtttccg agtcggcggg catctgcttt catgccagaa
    16501 aaagatgagt gaatatccaa tgagttacag tggaaggatt catctcatcg cggggtggtg
    16561 cgggcttgtg ctgatcgtcg gtggatgcac caccgtgctt ccgccagccg atcatggtgc
    16621 cgccaacgag tcgagccaac gctgcgtgcc tgcgatgacg aagcgggcgc gagtggctgc
    16681 ggccggggat gaaacgcctg tgacacccac cacggaagcc cgctccagat tcagttcaga
    16741 tgccttgcag gtcgcggagg tcgtgggtgt cctccccctg gtcaatcacc ttgcgagtga
    16801 caccggatca tcgcggcggg atcctctggc atccgtcgtt ctccgtcagc ggctcacgga
    16861 aatccttctg ctgaccctct ttgagatcac ctcgacgacg gccgaactca cctgcgaacg
    16921 tgatcgagcc gatcaagtgg cggatcgcat cgatgaaatc gatggagccc gggtgaagcg
    16981 gttgacgatc gcctcgatcc tcttcggggg aattgccagc atcgtctcgg gggggatcgg
    17041 gttggctgct ggcgctagcg tgggcgggga ggctgccgat gtggccggag gggctctggc
    17101 gtcctggttc ggggtgtcag ccttattcac gcattctgag gtggaatttc gtcatgagcg
    17161 caacgtgctc caggaaatct gggaagatcc ctcgcaaccg cgcctctttt ctcccaccat
    17221 ctggcggtac ttgcatcgag cgcatgacga aaagggaaag cttccgcggg agcaggtcct
    17281 tgactcatgg cagcaggcag gcaggatggg agaaaacggg tcaccctccg agcaggaacg
    17341 gcgccgctta tttttcggcg caggcggccg gtacggggcg gcggaattac atgcgcgcgc
    17401 gtctatgctg gagacgctgg aagcctcgat ccggttgctc tatgaagagt tggaagtgct
    17461 gatgcgtgaa ctggccgaag agaccgccga cgtctcatga atgtcaccag aggagtcaga
    17521 ccatggtttc caaacacacg aaaacgcggg attcaggact cttcgtgcgg tttgccaatg
    17581 aggcttcgcg ggtggtgggc agcgccggcg cgttcggcgt ggcgattgtc gtggtgttgc
    17641 tgtggagcct gtcagggccg ctgttcggtt acagtgatac gtggcaactg gtcatcaaca
    17701 cggggacgac cattgtcacc ttcctcatgg tgtttctgat tcagaacacc caaaaccggg
    17761 atagcgccgc cacgcagctg aaacttgatg aactgatccg ctcgacacaa ggtgcccaca
    17821 acgcgttgct cgacctggaa aagctgagct cgaaagacct cgaagacatc cggatgctat
    17881 atcagaagtt ggcggaccgg gcccgccgcg aaaagaaagg cgggtgggaa gacacggata
    17941 cgcccgagct caagcgggtg tgaggccgat caagtcggcg tacgttcctt tcgcttgacc
    18001 ggcgcgagat cagtaatggc aggtccattc aggaccttcc cgaaccgatc gaagcgcgat
    18061 ccatgacagg ggcaatccca ggtgtgctcg gaatggttcc aggagacgat gcaattgagg
    18121 tgagtacaaa cagccgaaca ggtgtgcagc gtgccatcgg catcacggta ggccgcaatt
    18181 ttcgtgaggc cgtctctcag tactgcgccc tgatcgttgg caatatcctc ctcccgttcg
    18241 acatcccctt tcgtcaccca atcggtatat tgcgccacca cattgagcga ctcgcgcaca
    18301 aattctccca tcgccttcac gggctggcgg gaaggattgt agagcgatgc ccaagggcat
    18361 tcccggccca tgatgaggtc ggtcaggagc agaccggcaa tggttccgtg ggtcataccc
    18421 atcccgcagt caccggtcgc aatgtagata ttgtcgacat cgcccggatt tttaccgatg
    18481 aacgccaagc catcgaccgt ttccatcact tgtcccgacc atcgatagga gactgctccc
    18541 atggcaggaa aatattcgcg ggcccaggtt tccagacggc cgtagcgcct ttccgcatcg
    18601 tcggcctgac cggccttatg gtcttcgccg ccgacgataa gaaggagttc ctcgcgcccg
    18661 tcgggaaacg aatgtcgctg cgtccgcacg tagtgatagg gatcctgaat atcccaccac
    18721 aacgcttgtt caatggcttc agcgggaatc ggcgctccga tgacataggt gatataggcc
    18781 gcctgcttgg tatgaatcgc caccatgtta ttgatgggcg tattggtggc gacgacgacg
    18841 gcatccgccg tgacgacgca tccttgactc gtttccaccc gggcctgttt gccgctttcc
    18901 accttggtga catgcgcgcc gctgaatgcc cggcatcctc ctgactggag cgctttgaca
    18961 aggccggcca ggtatttgag cgggtgaaac tgcccctgat gcgggtatcg caggcaggga
    19021 ccgttcttgg ccagggggcc tgctggtctc gtgacatgtt cgacttcgct gaggcctgca
    19081 cgcaaaacgg ccatccattc gtctttgatg tgctgcgcgt cgtccggaga ggcagcgcag
    19141 agatagccgt ccacccgaag aaagtcgcag tcgatcctgg cgtctttgat ggtcttttcg
    19201 atccagtcga tggcggccgt atgactttcc gcagccaggc gggctcctga tcgaccgtgc
    19261 aaacgttcaa tgtccagata tccggaatcg atggcgttcg agaggtgtgc cgtcgttcgt
    19321 tccgtcatgc cgcttcccgg ttggctgtca tccaagagga tcacagaacg gccttccttc
    19381 atcagacaat aggccgtcgt gatgccggaa attccggcgc cgactacaca cacatcggca
    19441 tgcgcgccag tggatatagg tggaagttga gggatgtttt cggacatcca gatggaaact
    19501 gtgcgtccag gttcatcatg catggcggac tccttaggat ggtgcgcaga aggcaggcag
    19561 cggcctcgtg aatattggcc aagcctatcg ggctgcccca gagataacag ctagggatgg
    19621 gcctagttgt cgggtcgtag aagtgaagag atgattcgat cttcaaatga tttgcccttg
    19681 aacagggtcg ctctcacgag aaccagcatt catcgattga tggtctgtcc atacgattgt
    19741 cacttctgcg ccgatccaac ctgccgggtg gatgggtgcc agcttgtcga ggccacgatg
    19801 ttgctcgtct gcgacgagtg cggagagccg gtgctgtgcg tgaactgtgc gcatgtgtgc
    19861 gtcacttgta tgcaggcctc gccgcctcgg cctaccccta acaaataggt gccccatgcc
    19921 acgctccctc tggaaaggat cgatcagttt cggcctggtc aatattccgg tggtgctcta
    19981 ctcggccgaa aaccggaaca gttttgacct taccctgtta gaccggcgcg acatgaagcc
    20041 ggtcgggttt aaacgctaca acaaggagag cgggaaagaa gtcacgtggg atcaaatcgt
    20101 caaaggatat gagtacgaca aaaaccggta tgtcgtgctc accgatgagg atttcaagcg
    20161 cgccaatgtg gaagcgacac aaacgatcga catcgtcaat ttcgtgcggg cagaagagat
    20221 tgcccccacg tcgtttgaga cgccctatta tctcgccccg gataagcatg gtgagaaggg
    20281 ctatgcgctg ttgcgcgaga cgttgaaaaa gaccggtaag gcggccatcg ccaccgtagt
    20341 gatccgtacg cggcaataca ttgcggcatt gattccctgg agcgagatga tggtgttgaa
    20401 tacgctgcgg tatgccaacg aaatccgctc cagcaaggag ctcgatctgc ccgccaaaca
    20461 tgaaaagtcg gcacaggtga caccgcgcga agtggacatg gccatgaagc tcgtcgagga
    20521 aatgtccgaa ccgtggaaac ccgagcgcta caaagatacc tatcatgaag atttgatgcg
    20581 gttgatcgaa aagcgcatca aggccggtaa aacggaagtg gtcacgacac ccgggaaaga
    20641 ggaaggcggg gagaagcccg gtcgcgccaa ggtggtcgat ttgatggctc ttctcaagcg
    20701 cagtgttcaa cagtccggtc attccaaaca ccctgctcgt gagaaagagc ggtcgccacg
    20761 gcgtaagagc gcctgaggtc actcagattg gctgtagaca gtggagggct aggtatggct
    20821 ggggtcagca aagcggagcc ggaagcggag acggaacttg tgaaggatag ccatagccgc
    20881 gaagagaagc aggggctgac attgggcgcg caggtgacag acaaaggcgt gcgatttcgt
    20941 tgttgggccc cgcgggcggc tcgggtcgat gttgtcctag agggtcgcaa cgtcattcac
    21001 gcgttgtcca aacaggccga tgggtattgg accggactcg tccccgaggc ctcggcgggc
    21061 atgcagtatc gctatcggct ggacggagga caagcttatc cggacccctg ctcgcgtttc
    21121 cagcctgagg gaccccacgg cccatcactg attgtcgacc ccggcgcata cacctggcat
    21181 gatgaccggt ggcccggcgt cgccatgcat gggcaggtga tctacgaact tcacatcggt
    21241 accttcaccc ctgaaggcac attcgaagct gccatcgagc ggctcgacgg tttgaaagaa
    21301 atcggcatta ctgtcatcga agtcatgcct gtggcggaat ttccgggtcg ctggaactgg
    21361 ggctacgatg gcgtcggcct gtatgcctcg gcccatgtgt atggcgacgc gaacagcttc
    21421 aagcgcttcg tggacgaagc gcaccggcgt ggactcggcg tcatattgga tgtggtgtac
    21481 aaccacctgg ggcccgacgg gaattacttg ccgacattca gcgctgagta tttcaccgat
    21541 cggtatccga atgaatgggg gcaggcgatc aatttcgacg gccccggttc agggccggtg
    21601 cgtgattttt ttattcagaa cgcctgctac tggatccgcg aataccacgt cgatggattg
    21661 cgcttagacg cggtccatgc gtttcatgat gccagccccg tgcatgtcgt cgcggaattg
    21721 tctcgagcgg ccaggcaagc ggccggaccc cgttccatca ttctggtcgc cgaatgtgaa
    21781 gcgcagtggg tccagaccat tcagccgttg gcggaaggag gctgggggct cgacggcgta
    21841 tggagtgaag acttccacca tacggtgcgc gtggccgcaa ccgggcgcag cgagggctac
    21901 tacaacgatt atcgaggcac gccgcaggaa ttcatttcct gtgtgaaacg ggcgtttctc
    21961 taccaggggc agcgatatca gtggcaaggg aagccacggg gcacggtggt gtcggaagag
    22021 ccggccgaag ggttcgtgtt ttttctgcag aaccacgatc agatcgcgaa tcagttgtgg
    22081 ggcgatcgct tacatcagaa agtcagtcca tccgtgtatc gcgctctgac ggctctcctg
    22141 ctcttggccc cggagacgcc cctgttgttc atgggacagg aattcgcagc ctcctcgccg
    22201 tttttatttt ttaccgattt ccccccgggg caattagcga acgacattca ccaaggacga
    22261 aaacaattcc tgtctcagtt tcccagttat gcgtcgagtg ctgcgcagga cgcaatcggt
    22321 gaccccagcg cgccggccat gttcgagcgc tcgaaattgg attggacgga acgggagcgc
    22381 cacgccggaa tcgtggcgtt gcatcgagac ctgttgcgtc ttcgtcggga agacgatgtc
    22441 atcgcacaac agcggcgtga tcgggtggat ggggccgtca tcgggccttc ggcgtttctc
    22501 ctccggtatg ccggtcagca tggcgataat cgcttgctgc tcgtgaatct ggggcccgac
    22561 ttggattttc gtcctgctcc ggagccgctc cttgcgccgc caagcgggcg tatgtggacg
    22621 tttcgctggt ccagcgatgc cccgagttac ggaggaccgg gagtcatcga gccgttgacc
    22681 gaaaccggct ggaaaattcc cgggggtgcg gccttcctgt acgccgctct ggacgataca
    22741 tccaaggaca ctggcaagac tcccgcgatg gcgagcccgc atgaagagga atcgcgatga
    22801 cgacaatggc cgatgcgatg gtgctgacgg tccctccgga gcggctccgt gatcctaacg
    22861 cctttctcgc cagagaatgg ctggtgacga acggtctcgg cggctatgcc tcgcaaagcc
    22921 tgttgtgcgc tcccacacgg agataccacg gtctgttcgt gcctgatctg ccggcccctt
    22981 gggggcgcac ggtcatgatg cctcgttttg acgacgaagt gctggtcgat ggcgacgcga
    23041 tcttcttgag tggtgtggaa ttttccgatg gccggctgga aagcaatctt ctgtcggtat
    23101 tcgacggctt cagccgggaa cggcagacgc cagtctggcg catccgggtc aaaggccgcc
    23161 ggctggaaaa gcgcgtgatc atgccctatg gccacaactc cgtctacgtg gaataccgcc
    23221 ttcttgaggg tgatccggtc ggcctgcggc ttcgcccgtt cgtcaccttc cggatgcttg
    23281 atgcgagatt gcacgatgcc aagcgaccgc ctttccctct caccgtcctg aatggccggt
    23341 acgacatgca tctgtgcgaa ggagcgcctt cgttaaaatt gtgcttgcgt ccccacggag
    23401 ggaagttcgt ggcggattac gagctgagtg tcgatgtgtc ctaccgggtc gatcgcgatc
    23461 gaggctcgga gcatatcgag agcctcgcca gccccggcta ttttgtggcg gaccttacgc
    23521 aggatcggcc cctcgcgttt gttgccagca cggaaccgtg ggaactgctc gaattcgacc
    23581 ccgaagccat ttttgaggca gaggcccaac gcctggataa attgctgtcc caacggggag
    23641 gcgaagcgga tgaagacatc gaaggttggc tgaccatggc ggccgatcag tttgttgtcg
    23701 ccccgggctc tcgactggag gagcaggcgc tggcgcgtgc ctccggaaat gaagcgcgca
    23761 cgattatcgc ggggtaccat tggttcacgg attggggccg cgatacgatg atcagcttgg
    23821 aaggtctcac actctgtacc gggcgccatc aggaagcccg agccatcctc cgaacctttg
    23881 ccaattatgt gaaagatgga ttgattccga atctcttccc cgaaggagag cggatgggcc
    23941 tctaccacac ggcggatgcg acgctgtggt attttcacgc gatcagccgg tattaccggg
    24001 tcacgaacga ccgtgagacg ctgttgacct tgttgcccgt cctccggtcc gtgcttgagc
    24061 accatgttcg gggtacacac ttcggaatcg gtgtggatgc gaaagatgga ctcttgcgcg
    24121 cctcggcgga aggttaccaa ctgacctgga tggacgcctt ggtggatggc tgggtggtga
    24181 caccccgtcg gggaaaaccg gtcgagatcc aggcgctgtg gtacaacgca cttcggtgta
    24241 tggaggagtg ggaagtactt gccggtcaga cgccgtcccg ttggagagac ctagcggaac
    24301 aggtgcaacg ctcgtttaac gagcggttct ggattgccgc agagggctac ctctacgacg
    24361 tcatcgatgg cgagcgaggc gatgatacgg cgttccggcc gaatcaagtg ttgtccatcg
    24421 cgctcccgca tccggtgctc caggaagatc gatggcgaag agtggtggac ttggcggcag
    24481 accgactgtt aaccccggtg ggattgcgaa gcctgtcccg cgaccatccc gactataaac
    24541 cgatgtattt cggcgatctc cgtgcccgag acgcggcgta tcatcaaggt accgtgtggg
    24601 cttggctgat cggtcctttc atcgacgctt ggctcaaggt ctacaaagat aagtccaagg
    24661 cgcgaggtat gttggatggg ttccgttcgc acctgctgga tgacgggatc gggacggtca
    24721 gcgaaatttt tgacgcagaa gctccctttc atccgcgggg ctgcattgct caggcctgga
    24781 gcgtcgccga ggtgttacga gcctatcgaa acacgcgagc cgagaaggag agggtgtgag
    24841 ggattgtggg tgtggcgcgt atcggtacgt cgggatggca ttacgcacat tggcgcggac
    24901 cctattatcc gcccagtgtc ccgccgcgag cgatgctgga ttattacgcc cgtgatctgg
    24961 ataccgtcga gatcaacaac agtttttacc gcctgccccc atcggggacg tttgagggat
    25021 ggaagaagtc gacgccgccg ggtttttgtt tcgcggtcaa agccagccgc tacctcaccc
    25081 atcagaaaaa actgctcgat ccttcgcctg cgctgaaacg attgctgggt gcggcagtgc
    25141 atctcggtgc aaagttgggc ccgattctgt ttcaattgcc tccccattgg cgatgcaacc
    25201 tcgaacggtt gagcgacttt ctgcgcacgc tgccgccttc gcaccggtat agcctcgaat
    25261 gccggaatgc gacctggcac acgggcgagg tctaccgtct tctggaacaa cacaatgtgg
    25321 ccttttgcct cttcgaactc ggtggatgca tgactcccat cgaggtgacg gcggatttcg
    25381 tctacgtgcg gctccacggg cctggcaata aatatcaagg cgattatccc acggcgacgc
    25441 taaaagcctg gtgccgtcgc atccggcaat ggacccgttg cgacaaggat gtgtatgtgt
    25501 atttcgataa cgaccaggcc ggttatgcga tcaagaacgc gatgtctctg cgggcgatgg
    25561 tcgatggttc ttaattgagt gattgctgag gcgagcgatt ggcggacgtg aaatattggt
    25621 cgctagggcg aaactggacg acgggttgcc gcgtcgggcg tgcccggatt ggtcttcatc
    25681 ccctcatagg tgctgcgttc atcttccagt cgcacctgtc catagatcaa gtagccattg
    25741 tatgcgcgcg cggtttctct ggcccagccc atggtcaaca tcatgagcat ggcgcatagg
    25801 gctaacgtga tcaggagcgc gggactcgac gactctttct ctgagccgct ctgaccctgc
    25861 caccatggaa ctcgattggc ataggatcgg aagaaaaaga gccaattcat gaggccgaag
    25921 accacaagaa acgcgagggc gaaatatttg ttcggttgca tctttcccaa ggggttgatg
    25981 aggcggtcgg tgaggatctg catccccggg atgtgttgca gttggtaggg ttgggaaaga
    26041 atcatacccg caattgcaac gataagcagc gacatcggca gtgccagcct ggctgttcct
    26101 ccctcgtcat tcgctggctg ggcccgtacg ctccgccaga tgtaccagga tccgaacagg
    26161 acgaggagtg agtagagggt tgcgacgaga tcgaagagcc aggagcgggc gccgagcatc
    26221 agcgtgtaaa acgcctgcgg ttcgatgtgg cggactctga gcagatattg gtacccgatg
    26281 gccggcatga tgagcagggc gccgaagccg atgatgaagc agacccctcc tgcccaccgg
    26341 taaaacgcgc gatcgtcttc gcctcccgaa cgtagaaatc ccgccgcgca gaggcccgcg
    26401 agaccgaatc cggtccaggt cagattgccc acgatccgat gcgtgtccag atagagcatg
    26461 gtgggattaa acagtttcgc ccaggtgtca tcgggtgggc ggggagtcaa catataggaa
    26521 gcgaccatgt cgatcatcaa catcgcgatg agggaagagg cggcggcgga ccacccgaag
    26581 acaaggtgcc agcgtttgcg cgccggctgc tggttcgccc aatcccagga gtaataccag
    26641 gtgtaggcaa agatggcctc cgccagaaag aatgatgctt cgagcagaag gggccagaac
    26701 ataatatgaa acaactgact ccagaatcgg ggccagaaga tgttcaagag gaagacgaat
    26761 gtcacaccga gaaaggagat tccggcggtc gtgagaatga gtgtgaaggc aatcaggcgg
    26821 gagagccgtt cgaagcgaag gttctgcgtg acatgcccga tggtttccgt cgccgagccg
    26881 atcaccgagg agccgacgat gaaggtcgcg cagagcgcat gggccaaagc tgcgactgct
    26941 atagccaccc gtccgtagag catgggaatc tggagcgcct ccatctccat cagcggagcg
    27001 ctccttccat gcgtgcctga tgccgcaatt cgtcctgaag cgaaattatg ccgcggacgg
    27061 tgtccggtcg ccgggaggct tctctcatga cacccatggt gaggtacagc agaaaggata
    27121 agagagccag gccgacggcc agtgagcgca ccggtgacgg agagagagcc gattctgtgg
    27181 gtgccttgga cacggaggcg gccactcgcc ataccgtcac gccgacgagc aggtatacac
    27241 agacccggcg gagttccggg tagccggcag agagtaggag tagcgctgcc agaacaggaa
    27301 agccgatgtc tagccagcgt tgtctgttgc cagtcagcgt ccgcatgcgt ttcgtcaaga
    27361 catcattgcc gaggaagaac agagagagga ggagcaattg gagatagagc catggcacgt
    27421 atgggccggt catgatggat gcctgggcgt ccggtgctgc gcgatggatc gccatcgcat
    27481 acagcaggcc cgtgaagggc tgcaacagga acgcgaccaa tccgatcacc cagcctctgg
    27541 agagcagatg ttggtagtaa ggcttgagag caggctcgcc ggggctccaa actcgccagg
    27601 cgccgtaggc ggcaagggta tatcctgcga tcatcagatt tccgaccatc cggtggatga
    27661 cgagcggcag ccaggtggga gtgaagagtc gttcccacgt ccctccctcc tgcaccggcg
    27721 tcagcatgaa ggcacccatg ccgtccagca tgaagaccca cggcagcatg aagaaagcgg
    27781 ccaaggctcc cagaaccatg tggagcgtga tactggttgc tcgtaagcgg tcccagaaat
    27841 ggtagtaggg atacagggcc agcagcatca gcatgaacga ggcgacgccg aatagaatcg
    27901 ggacgcgaaa gtgattccac atccacatcg tggtgaccgg gaagagaccg atcatcagtt
    27961 ccaccatgat gacggcgagc acggtgctga cactgaacac caccacggtg actcgtgtca
    28021 caagaagcgc cagctctcga tcaaagggat ccgtccgccc tctgaattca aaaacgggcg
    28081 ccatcaacat aaaggccacg gaaagcgcgg ctggaacgat atgcagcagg ctgaacaagc
    28141 ccaccgcgac gctatttccc agaagaggaa tctgaatggc aggcagcacc gttccataat
    28201 taatcatgtg accgtcgttc ctcgtgagcc atggtggtgt ctggctccat ggcatggacg
    28261 aatgacgcca gaatcatgcg ctcggtggcg gacagctcgc cttgaaagga aggcatcatg
    28321 tccttcccct gcgtaatgat ccggtagagt tctgttttgg acagagctcg cactcgaggt
    28381 gaccgcaggt ttgccggcag ttcgtgcaaa tagggcgcga caaggcccgt tccttctccg
    28441 ccctcgccat ggcagggcag gcaattgatg cgatacagct caggggcgcg ccctccttcc
    28501 agctccatgg tgcgagcttc ccgggacaaa atggcgcgac tgtcgcgagg gacgcttccc
    28561 gtcggagaat ggaggcgcgg ggcttcctgg cttcgatagg acggctgttc ctgcatatcc
    28621 ggggaacagc cccccacggt gatacagatc agaagccatg cggccgtcac atgagcccgt
    28681 tgcatccctt gttgaatcgg cgaagggtga gtcaccagcc gaacgtcctc cgcgcctgcc
    28741 atttcgagca acgttttgac gcgctgttcc tggccgtctt cgacgagacg cactgagagc
    28801 gcaatctttc cgtcatcgat cctggtctca cgatcaacgg aggctcgccg tgcccgtctg
    28861 atcctgacga tcatcgtgat aaacgtgacg acgatcgcca acaacatcat cgtttcatag
    28921 gaaatgatgc cgacgatggg agcggacaca atcggtttgc ctccggtcat caagggatac
    28981 aggagcgcag tgccggcggc gaagaagact cctacgccta tgcccacaag cccggcgacg
    29041 atcgtgatga tgtaaggcca caagggctgg ctggtaccat ctgttgcgga agccgacttc
    29101 aacggcaccg gggtcaggag tgcgatctga tcctgggaga tgccggatcc ctccagcgac
    29161 aggcgcacag aatcgagatc ggcatcaagc ggaaagagtg cgatcaggcc ttcgcaaggc
    29221 tgagtcctgc tcatgcaacc gtccttcctt ttcttcccag atggaaatga tcggaacgaa
    29281 cttgatcgcc agcacgtaga ggagcaccgc cagcgaaagg gaaccggcca tgattgtcag
    29341 ttcaacccac gacggaaaat agtcgctcca attgtaggtc aaccggggat gggccaacgg
    29401 cggggtgaga atcagcatcc gctcgatgta catgccgata ttgatgcaga ggccgatcac
    29461 cagcatgccg gccggccacg tgcggaaggc gggtattgtt aaggtgatca gcggcagtac
    29521 atagatcaac gtgatcatgg tccattgagg aacggcatag ggtccgctca tgactgcatg
    29581 catgaccgac agatgatccg gtatcgctcc gtaccagacc gtcagctggt ctgcgaaata
    29641 gaggtaggcc agcgtcagcg tcatcacgat catgagcttg ccgagtttgt cgaaatgttc
    29701 caggcgaatg taagcgtcga gtcgatagat tcgccgcacg aggtacaaac cgatgacgac
    29761 catgcccagt cctgaatgca ccgcgcccac cacgaagtag ggggcgatga tcgacgtgtg
    29821 ccatccgggg accagcgaca tgccgaagtc ccagccgaca atcgaatgta ccgagatcat
    29881 gaccatcaca atcaggggag tgatgatcct gatcgcagtg ttcagcgccg tccattccct
    29941 ctgggaacct cgccagccga ggctgagtaa ggaatagagc ggcttgcgcc atccgccgat
    30001 gcgatcgcga accaatgcaa aatccggaat cagcggaagg tacagataga tggtgctcgc
    30061 ggtggcatag gtaaagacgg cggtggcatc ccacatgagc ggagaacgga agtccggcca
    30121 gatttcacgc tggttgggat agggaatcag gtaataccaa cgccaggatc ttcccaggtg
    30181 ataaaagacg aacagggtcg caatgaccac agagatgatc gtcatcaact cggcgaccct
    30241 cgtaatcggg cgtcgccatt ccgccttggt cagtcgaagc accccggaga taaaggtgcc
    30301 ggaatggctg acgccgatcc aatagacgaa ggaggcaatg taggcactcc agaagatggg
    30361 aggatgcagg ttcgtccggc cgatgccggt ttcgatttga tagacccagg ctccaatgcc
    30421 ggcggatacc atggcggcca gcaatgtgac caggagcaca tacccgcggc ctcccgctga
    30481 aaggggcgcc aacaggtctc gattgatggc cggcatctct tcccagccgg ctgtgctgtg
    30541 gttcattcat ccaaccgttt caaataggta atggccggat gcgttccgag cgattccagc
    30601 aggtggaagc cccgtccgct tttggccatg gtcgcaaccc ggcttgtagg gtcattgccg
    30661 tcgccgaata ccaacgcggt ggtcgggcag gattgagcgc aagcgggggt gatatcgcca
    30721 tcgcgcaccg ggcgaccgtc gcgctccgcc tgttccttac cctgtctgat tcgctgcacg
    30781 cagaacgtgc atttttccat gatgccgttt tgccggaccg tcacgtcggg attgagttgc
    30841 tgttccaacg gtggatccca gtggggatcg aaccagttga attgccgcgc cgtatacgga
    30901 caattattgc cgcagtagcg caccccgatg catcggttat agacctgggc gttgaggccc
    30961 tcaggggtgt gatacgacgc gtagaccgga cagaccggct cacaaggcgc atcggcgcag
    31021 tgttggcaga gcacgggcat gaacctcgct ttgacgtcgg gatatgcgcc ttcccaatag
    31081 cgttcgatgc ggatccaatt gatggcgcgg ccgtcggacg cttctccggg gccggaaatg
    31141 cggatattat tttcagcatg gcaggcgacg acgcaggcct cgcagccggt gcatcgatcc
    31201 aggtcgatca ccatctgcca ccggtaaggg cggggttcat ctgatggcct ctgcttctct
    31261 tccatgcttc agtcctttcg tcgaaacctc cggttcaccc agtgaaggcc gtccagctca
    31321 tgtgcgggcc ttggatgagg gcggccgctc tatcgagatc aggtcagagc ttccagccgc
    31381 ggtcgtgccc ggcttctcaa gaaggatcaa agtccccgga acgttcaacg gttcagcccg
    31441 gacccttgtc gcaccgatgg ccaaggcccc cgattgcttg tcgaataggg gcgcgagaat
    31501 cgtcatggga ttggctcccc gccgagcggc atatcgacca tagtccgtat ggccttggcc
    31561 gatgggcatc gcgatgacgt caggccgcag cccgggatag tgcagggcag gcgcctcgag
    31621 agatccatgg cgagagtgca ggcgcacgag tgcaccctgc tgaatcccca tggcctgcgc
    31681 cgtggccgga ttgatttcaa tccaacttcc ccacactgcg ctggtcatgg tatcgggcaa
    31741 ttcctggagc cacggcagat tggcgccgcg tccctgcccc agatgcggcg cggcgaaggg
    31801 gtataggtag aagggaaact cggaggcatc accgtcaaac tctggcgggc gatactccac
    31861 agcggaagac gactgctgca agcgttgttc gttggccgcc gagtcccacc agcctccctg
    31921 ttgaaggagt tgttcccaga ctgattgaaa cggttcatgg gagccggacg agtgggacgt
    31981 atgaaagtgc tgccagcgtt tcttgatgtg ctcaggaaac gactcgaccc cggcgtgctt
    32041 gcctgccaat tgacgtgtca actcaagcag cgtgtcgccc aacccgcgcg tgtcatacag
    32101 cggcaccaca accggctgca ccagactcgc cgcacggtac ggtgaggacc cgccctgaac
    32161 atggtcgccc caggtttcca accgatgatg gtctggcaga atcagatccg cactgacggt
    32221 ggactcatcg aggctgccgc tgaaactggc aatgtaggaa gcgcggtcaa atagacgtcg
    32281 aatagggatg gcaggtggaa cgctgaacaa gggattacag tcatagagca acaagagcgg
    32341 gcgttgcccg gtcttctcga tggtctccgc cagttccttg atgcggcgtt ctgacagcca
    32401 ggggatctcg ggatgaaggg taagtgaggc cgactgatac cagcggatgc ctcccgcttt
    32461 gttgatgttt cctgctaagg cattgagccc gtgaatggcc aggtgggttt cgaacgcgtt
    32521 cgtatgggca caggcagttc cgccgccgat tgccaggggc gagactgcct cggcaaatgc
    32581 tcttgcaagc tctttgatgt cagccgtgga aatgtccgtc tgcttcgcca cctcgtccag
    32641 agagagacgg ccatacaacg attcgtatgc atggagtcga cccgtgggca gactggaacg
    32701 gtgttccgaa agcaggatat gaccgatacc gcgcgcgaga agcccttcca tgccgggccg
    32761 gatgggcagc caacgatctg cgctggcggc cgtcagagac aggcgaggtt cgacgtgaac
    32821 gaaacgcccg cgtcgattct tgcgtccctg tcgcatccgg ccgaacgcga taccgaacga
    32881 caccggcgac agccactgtt ccagaaacgg tgcgccgaat gacaacacat agtccgcatc
    32941 gcccaggtca taagcaggaa ggtcgtgaat gtcgaatgcc tgtttcatcg ccgctcgcag
    33001 agccaattca ggcgacggat catagaagta caaggagccc tttacggaag cgacaaagtc
    33061 cgagacgacg tctgccagcg tgccggagag cggccggctg atcatgatga ccgggccctt
    33121 cgcgtggcgt atctggtctg ccagttgatg aagaccctca tcccacgaaa ccggcgtaaa
    33181 tcgttgatcc gcttccgtgc ccattcggcg gaggggaccc cggaggcggt cagggttgta
    33241 aaggcgttga agcgctgcct gtccctgcgc acagagcttc ccctggttga ccggatgcaa
    33301 aggattgcct tcgatttttt ttgcgcggcc ttccattacc ttcacgatga tgccgcagcc
    33361 ggcgtcgcat tctccgcagg tcgaggcata ccactcggcc acacccggaa tgatttcgtc
    33421 gtccgggagc acgtacggca ccaatcggcg aacctcttgg tcgcacccgg gaagaagtgt
    33481 cccagctgcc gtcagcccca gtgctttgat aaacgttcgc cgtttgacat ccattcgaat
    33541 cccacatcca tatgatcaac gcggcagccg atcatttgtg gcaggtcacg caatcgcgtg
    33601 aagccccacg ttgctgatga cacgagaggc accagcccat ttcatacgtt gccgcgcgtg
    33661 gtgtgctcgt catacgcgtc acgtcgccat ggcatgtggt gcattcgagg ccctgggcca
    33721 gatgacgttc atgggtgaaa tagacaaaat ccggcagacg ttgcagtcgc acccactcca
    33781 ccggtgcttg ccgcttccaa tgatcgacca atgtctggat gcctgctgtc gcgacatcca
    33841 ggttttggtg acaacccatg cacagctgga ggcttggaat cccggccgcc ggagagatat
    33901 gcgcggccct gtggcagtac cggcagtcaa gggcgagttc gccggcgtgg aaggcatgat
    33961 tgaatgcaat gggctgttgg tcgtgggcgg cggagtcagc gctgcctttc gagtacagaa
    34021 tcccaatggc cagcgcgctg acagtggcca gcgagaccag accaagactt tgccacaatg
    34081 aacgtgtcat cggacggcaa ggaacattct cgtagcgcgt tccaactcgt tgttgtctca
    34141 tctattcaga ggctgagaga cggttctact aggcattcct cctgagcccg cgtcgaataa
    34201 ctaccgaagc ctctctgcta gggaaccctc tagctcaaag cctcgtccgc cagagctaca
    34261 gtgggtggac acgtctcgac ggtatgagaa ccgtcgaagg ctcgaatctt tcacgaactt
    34321 cacacggtct aaagatgcct ccacggacta gagatgcgga acgacgtgtg atggagcgag
    34381 atgtctgctc gaccaatggc gactccggcc cgaaaatcga aaaggttcag gtctctgcct
    34441 acacaattcc cacggccggc ccggagagcg atggcacact cgagtgggat cggacgacgc
    34501 tggtgctcgt cgatgtgagc gcagggggcc agcacggcat cggttatacc tatgccgatg
    34561 tctcgaccgc gtggtttgtg aagcagaacc tggcgcctgt cgtcgagggt cgtgatgcga
    34621 tgcacatcgc ggacgcctgg tgggccatga tgcgctcagt gcggaatctc ggccatccgg
    34681 gagtggcttc aatggccatc gcagcggtgg acaccgcctt atgggacgtg aaagcacggc
    34741 tgctcgatct tccgttggtg tcgctgctgg gccgcgtccg gacagcggtg ccggtctatg
    34801 ggagcggtgg gttcacctct tattcgatcg atcggttgga agagcagcta ggtgggtggg
    34861 tcagggacgg catttcacgc gtcaaaatga aagtcggccg tcaaccggat caggatcttc
    34921 gccgggtgga ggctgtgcgg aagacgatcg gagaggaggc acaactgttt gtcgacgcca
    34981 acggcggcta cggccggaaa caggcattgc agctgtccga aggctttgcc aaggccgggg
    35041 tcgtatggtt tgaagagccg gtctcgtcgg acgacacgga ggggctgcgc ttacttcgtg
    35101 atagggtgcc ggcgccgatc caggtgacgg caggggaata cggatacgat ctcttctatt
    35161 ttcgccgcct gctcgatgcg gaggccgtcg acgtgctgca agcggatgcc agccgctgcg
    35221 gaggcatcac gggattcctc aaagtcgggt cgctctgcga ggcacgccca gtcccactct
    35281 cggctcactg cgccccgtct ctgcatgtcc atcccggctg tgcgtcgatg tcggtgtgcc
    35341 acatcgaata cttttatgat cacgttcgga tcgagcagct gttgctcgat ggcgcgatac
    35401 gccctgtcgg cggggctctc tatccggatc tctctcgtcc gggaatcgga ttggacttca
    35461 aatatcagga tgctcgccgg tatcaggtgt gacatgcgtg agcccctcat tcacattcag
    35521 tccgccggtc aggattcaac tcaaggggct tcgtatgcgg cggaaccgag tgatgcgatg
    35581 cgggaacacc tggcgcggcg catcgatgca cagggattgg ccgaggcgct gcgaaagaac
    35641 atcgatggcg aagtccggtt tgatgaaggc agtcgtgccc tctatgcgac ggacgcatcc
    35701 aattatcggc aggtgccgat tggggtggtg attccccgcc atgctgacga cgttctgcgg
    35761 accattgcca ccgcccgaac gtatgacgca ccgatcctcg cgcgaggtgg cgggacaagc
    35821 ctggccggac aatgctgcaa ttacgccgtc gtgcttgatt gttccaagta tatgcatcgg
    35881 ctggtcggca tcgatgtcgc gggtcgatcg gctcgcgtgc aaccgggcct cgttctcgat
    35941 gatcttcgcc atgcggccga acaacaccat ttgaccttcg ggccggatcc ctccactcac
    36001 aaccattgca ccctcggggg aatgatcgga aataattcct gcggcgttca ttcggtcatg
    36061 gccggtgaga ccagcgagaa tgtgatcgaa atggacgtgg cgacctatga cgggctccgt
    36121 ctgcgagtgg ggcgaacgtc ggatcatcag ctcgatgaaa ttatcgctca cggtggacgg
    36181 gaggcggatc tgtaccggcg cctcatcgct ctgcgtgacc ggtacgccga cctgattaga
    36241 tcgcgcttcc cgaatattcc ccgccgggtt tccggataca accttcccgc gctgctgccc
    36301 gagcatgggt tcgatgtggc caaagcattg gtggggtcag aaagtacctg cgtgacagtg
    36361 ttggaggcca cggtgcggct ggtttccagt ccgcgtgtac gttccttgct ggtactgggt
    36421 tatccggacg tgtatgccgc cggtgatcat atccccgata ttctccgcgc gaagccgatc
    36481 ggccttgaag gaatggacga tcggctcgtg gaggacatga ccaaaatgaa aattcacccg
    36541 gacgacgtca agttgctgcc tgaagggcgg ggctggctgc ttgcggagtt cggcggtgag
    36601 agcaaggacg aggcggatgg gcaggcgcat cgctgtatgg aactcctcaa ggccgccgga
    36661 caccccccct ccatgaagtt gtttgatcga cggcgagacg agcaggtcat ctggacggtc
    36721 cgtgaatcag ggcttggagc gacggcccat gtgcctggag aacaggtgac ctgggaaggg
    36781 tgggaggatt cggctgtccc tgtagagcgc ttgggcgatt acctgagggc cttgcgccgt
    36841 ctgttcgaga aatatcgcta tgcctgtgcg ctgtacggac atttcgggca aggctgcgta
    36901 catacgagaa tcgattttga tctgcaaacc aaggaaggga tcagccggtt ccggtcgttt
    36961 ctgttcgagg cggccgatct cgtcaagagt ttcggcggtt ccctttcagg agaacatggt
    37021 gacggccaat cgcgcgccga gttactcccg cgaatgttcg gaccggaact cataggggcg
    37081 tttgaagaat tcaaggcaat ctgggatccc gatggaaaga tgaatccggg caagatcgtc
    37141 cggcctttcc gtgtcgatga aaacctgaga ctggggagcg actaccaccc gccggatcct
    37201 cagactgatt ttacctttcc gcaggatgaa tatagttttt cacgagcgac gcttcgttgc
    37261 gtgggggtgg gtgaatgtcg ccggcaggca gggggcacca tgtgcccgag ttaccgcgcg
    37321 acgcgtgagg aactccactc gactcgcggg cgggcacgcc tgttgtttga aatgctggaa
    37381 ggcgatcccc tcacgcgcgg ctggcggaat gatacggtga aagaagcgct cagcctctgc
    37441 ctggcctgta aggggtgcaa gggggattgc cccgtccatg tcgacatggc gacctacaag
    37501 gccgagtttc tcgcccatta ttacaaaggg cggttgcgtc ctcgatcggc ctattccatg
    37561 ggattgatcc accggtgggc tagatgggct tcgcgggcgc ccggcatggc caatttcatc
    37621 acccagacgc ctggattgtg cgacgtcgtc aaaatgctgg ggggtatttc gccccaccgc
    37681 agaatgcctc gctttgcagc cgagacgttt caggcatggc atcgtcagcg cgcgaaacga
    37741 gcaggcggtg ggcaaccggt acttctttgg ccggacacgt tcaacaatta tttttacccg
    37801 gagacagcca gggctgcgac cgaggtgctg gaggctgccg gatgtgccgt gttcgtgccg
    37861 cagaccaatc tctgttgcgg tcgaccgctg tatgacttcg gcatgctgcg gcaagccaag
    37921 cgacacttgc agcagatctt gtgtgaatta gcgtccacga tccgtagcgg tatgccgttg
    37981 gtggtgctgg agcccagttg tgctgccgtt tttcgagacg agttgggcaa cctgttccct
    38041 catgacgagg atgccagacg cctggcgaaa caaaccttca cgctggcgga atttatcgac
    38101 aaacgtttgc cggactggga actacctcaa tcgcatcaat ccgtgacggt gcatggccat
    38161 tgtcaccaaa aggccgtgat cgggctgggt gcagaagaac gcgtactgga tcgattgggg
    38221 ctgaatgtgc agatgctgga taccgggtgc tgcggcatgg ccggctcctt tggtttcaaa
    38281 caggagcact acgatatttc catgcagatt ggagaattgg ccttgctccc agcgatcaga
    38341 aataccgcta cagatacgat tgtgatctcg gatggcttca gctgcagaca acagatcgag
    38401 caatcgacgc accggcaggg gatgcatttg gctgaagtga ttcatcaggc aatgggcaac
    38461 gtaacaatgc acgtgtcggc ctcggctcac ggtgggactt aaccatgaat caggggccgt
    38521 gccgatacag agtggaagtg attgctcgat gacgtcctcg aagacattga tcgcggtggt
    38581 gctcatttgc ctgatcggga cgggtatgct gaatagccgg ccggttttag gcgaatggta
    38641 tgtcgccggt cagttcggag ctaattttgc cgatcaactg agtgacattc acggaaccgg
    38701 caatctcagc ggctttcgcg cgccggattt tgatttgcag aactcgctgc tgtatggagc
    38761 aaaggtcggg aactttccgg gaaacggatg gttcggtctg gagctagacg tctttcacag
    38821 tacgccgcac gtcaagaacc tcgatgatgt gccgggcttt cacatgcggg tgacgaacgt
    38881 cggcgtcaat ctgctggctc gataccctgg agtgaccttc cagccctatg ctggaatcgg
    38941 cgcgggagtg gccatcgcgc gaatcggcga ttcggctacg accagaggca gtaccgatgc
    39001 atcttcggcg ttgaatttac tggtcgggat cagggcattc gtgacgccat atgtggccct
    39061 gtttaccgaa tacaaataca gccaatccac ctttgaattc agtcacgcgt tcggcccttc
    39121 gagtgggttt aatggaaatt atgatgccca catcttggtt accggcttgt cataccattt
    39181 ttaagaaacg ctattgggcc acagcagcgc tcttcctgtc tgcgtggctt ctcagcgggt
    39241 gcgcgcaaag tgcgcatcag tccgggcgcg atctgctgga tcagattctg ccggatcagc
    39301 tgcagcaaca gattgatccc actgtcacgt ttccagagct gaaagccaat ccttccgcct
    39361 atcagggcaa aacgatcatg gtcagtggca ttgtggtgaa gagtaagcgg aggaaggatc
    39421 agacggagat tgaggtcttg cagatcccga caggaaccgg ggctgtgccc agcaaggatc
    39481 gtgcgcactc tcagggaaga tttcttgccg taaagagcgg tgaatttctc gacccagccg
    39541 tagtcgacgc agggacgccg gtgacggtcg tcggtgaagt gggaccgccg gtcaccaggc
    39601 cgatggacga cggggaatac acctatcccg ttctcgccat cacacatctc gttgattggg
    39661 atgaagttaa tccagctatg cgttccgccc cgtatgccta tccgtatgga cgctcgatgt
    39721 atgcctatcc ctatgcctat tgggggccgg ggggctggta cgggggctat cctgggtatg
    39781 ggtacggata tccctattac ggtttctatg gcttcggtgg aggatcatcc gcgccggccg
    39841 gcccgccacc ggcagatgct cctccgcagt tcaataagtg atggggcaat cgaccatccc
    39901 ttcgcaggag tgacatgagt cttcgtcaat actggaagaa acgaaacttc cgcaacactc
    39961 ccgagccgcg aggaaaagaa acgccggcgg gagcccggct ccagtttgtc attcaacaac
    40021 acgccgcaag ccggctccat tacgactttc gcctggagtt ggatggcaca ttgaaaagtt
    40081 gggcggttcc caaagggccg agtctggatc cggcacagaa gcggctggcg gtccatgtcg
    40141 aggaccatcc actggactat gcgtccttcg aaggcatcat tccggagaaa cagtatggcg
    40201 cgggcacggt gttgctctgg gatcggggct actgggaacc gatcggtgat ccgaaagcag
    40261 actatcggcg aggccgactc aaattcacct tacacggggt aaagctgcag gggatctgga
    40321 atctggtacg catgggcggc cgcgaagagg cagggaaaga gaattggctg ctgataaaag
    40381 agaaggacgc tgaagccaag cgcgggacgc agagccgggt gactgaccgt ttgacgagta
    40441 gcgtcgccag cggcaggagt atggagcaaa ttgccaccgg agagcatgcg gtgtggcatt
    40501 cgaaccggtc ctcgaccaca tcggcaaaga aacgtgccgt ctcccgcccg gtagagaaaa
    40561 ctcctgcggc cggcgcccgc aaggccgcac aggaagagtg gatcgttcca caattggcga
    40621 cattggtgac tgcttctccg gaaggggaag actgggttca cgaactcaaa tatgacggat
    40681 acagaatatt gtgccgcctg aaagacggtc aggtgaggct cctgacgaga aacggccatg
    40741 actggaccgc gaagctgcct catatcgccg cagcactaac agatttgccc gcacaggcgg
    40801 cctggttcga cggcgaggtc gtggcgttgt cgccggatgg tcgcatcagc ttccagctat
    40861 tgcagaatgc gttcgatgcg cactcagaca gtgatctgct ctattacgtg ttcgacctgc
    40921 tctatctgaa tggctacgat ctgcgggagg tgccgctcct ggagcgcaag cggcttctgt
    40981 cggctctact gaaagaccga ccgtcgtctt ccttgattca gtacagcgat catattaccg
    41041 gcaggggcga cgtcgcattc gccgaggcct gccggagcgg gatggaggga ttgatcgcca
    41101 aacgagccga tgctccgtat ctttcgggca gaaaccgcaa ttgggtcaag gttaaatgcg
    41161 ggcagcggca ggaattcgtg atcggaggat ttactgaccc ctccggttca cggagcgcct
    41221 tcggtgccct gcttcttggt gtttatgatg cgcaggggcg gctgcaattt tccggacgca
    41281 ctgggacggg atttacggag cgctccctca aggaattaca cacgcgcttg aagaagatcg
    41341 agcagaaaca gccggcattc gtgaatccgc cggtcggcag tgaggcgcgt ggtgtgcatt
    41401 gggtcaaacc tctcctggtt gctgaagtag cctttgccga atggaccaac gaggggcagc
    41461 tgcgccaggc ttcatttcaa ggtgtacgag aagacaaaga cgcccgttcc atcattcatg
    41521 aacgacctga ctcaggccct tcttctccga aggcacgcaa ggggcgggga catgctcgct
    41581 cgcgccggat ctcatcaaat cagcagactc cttctgccgg tgccgaagcg gatgaaaccg
    41641 cgccggtcat tggcggtgtc acgctcagtc acccggatcg ggtgttgttt cccgagcaag
    41701 gcatcacaaa aattgcgctg gctcggtatt acgagtccat cagcgattgg attctgccgc
    41761 atgtcgaagg tcggccgttg accctggtcc gctgtccgga ggggcacaag aaggagtgct
    41821 tttatcagaa gcacgcaggg gagcacatac ccgacactgt gggccgggtc gagatcccgg
    41881 aagaaaaagg ctcgtcctgg tacatggtgg cggatgatct tcccgcgctg attgggctgg
    41941 tgcagatggg gatattggaa cttcatacct ggggggcaaa gcgagaccag ctcgaacggc
    42001 cggaccggat gattctggat cttgatcccg atcctgcggt cgaatggcca ttggtgattg
    42061 aaggtgcgca attgctgcgc accttgctcg aggagctgga cctcgtctca ttcgtgaaga
    42121 cgacaggagg caaggggctg catgtcgtcg tcccgttgca acgcgcccat acgtgggatg
    42181 aggtaaagac cttctccaaa gccgtggctg atcatctcgt ccgcgcgatt ccggaacgat
    42241 ttctcgccaa catgtcgaaa cagaaacgca agggcaagat ctacgtcgac tacttgcgta
    42301 acgcccgagg ggccacggcg gtggccgcct attcaactcg cgcgcggcct cgcgcgcccg
    42361 tctcggttcc gctcgcctgg aatgaattgt cgaccgatct ccgttctgac catttcacag
    42421 tcgacaatat tgccgaaaga ctcgagcatt tgcgacagga tccatggaag acctacttca
    42481 ccacgaggca acgtattacg aagaatatgg tgcgccggct ggcaggataa tgtcgaagct
    42541 ccgccagggt gctttgctag gggacctggg gggagtgcta gtagagtgaa tcgtacggga
    42601 ctgttatcag tgatcatgac aatcggtgga agacattccg ccgaagccta tcaggatcac
    42661 gacgaaagga gaaccgtatg aatgccacca cgacgagaac ccttcctctc acgggcaaaa
    42721 ccagatctac tcctgctgtt tcccaacacg atacctgccg atgcctccgc tgcggcggat
    42781 tcctggtcga tgaacgctgt atggatatcg gggagagcct tggtggttac tggtttatgg
    42841 cgatgcgttg cgtacagtgt ggcgacatca ccgatgaagt cattttgcgg aaccggtctg
    42901 cgcaactggc gacgacgact cagcacgagg tggtgagagc cgcctgacgg tcaatcggta
    42961 gagggttccc tggaagagta gttgcttctt gaagtctggg cgagaacggg tttcgcacgt
    43021 tcctgtgcgg ggcggctcag acaggaaagg ccatcgtgct gaagagcgca ttttcagcct
    43081 gccgggaatc cagcctggta tgttgcacga cgatgcggac gttggaatcg atcaggctgg
    43141 agaaatccgt atccagagga atcggggctg gatcagtcaa atcgttaaag cggacgtgtt
    43201 ttgttcggcg ggcgggaacc tgaataatat agggaccgac cggttcgcga tccgcataaa
    43261 aaatcgtgac ctgcacggtc gcatcctggt ccgaagagtt gaggatgcac atggtttcgt
    43321 ggctcgtcat ttgtggttcc ggcccgtggc ttttggatgg aatatatcct tccgcaatca
    43381 cccatcttcg gcggccgatc ggctccatag gctctccccg ttaagtgcca gtacacgcgg
    43441 ttgcttcaca ccacttctct gattttgctc tcgaccaacg ttttgatgat gccccatcgg
    43501 tcatcctgcc ctttggctaa ggccttcgca aactgccagg cttgcgtcgt cgtaattttc
    43561 ccgggtagcg gtggttccaa cggatcaatg accgcctgga ccacggccgg cccttgaacg
    43621 gcgaaggcct ctcgcagcac ctcacggacg tcgcccggat cgtcaaccgt caatcccgtg
    43681 gcgccacagg ccttcgcaaa gagcgcaaag tcgatcggtt gaagatcgac gccatattgc
    43741 ggattgccct cgaacgccag ctgttcccac ttgatcatgc cgagcagatt atttttcagg
    43801 acaatgattt taaccggcag gccatacctc accagcgtgg ccagttcgcc catcaacatc
    43861 gtgcagccac cgtctcccgc gaggcagacg atctgtcggc caggatacgc aatacccgct
    43921 cccagactat aggggagccc attgcccatc gacgcgagtg tgccggatgc tgaaaattcc
    43981 atgttctccc tgatgatgat gtgccgggca gcccaggtgg tcaccgtgcc tgtgtcgcaa
    44041 cagatgatgg cgtcgtcggc gaggaactcg ttgaggcctc gcaccacgac ttgtggttta
    44101 agaggagtat ccgttctggt gcctcgctcc tccatgagct cattccactg ctgcatgctg
    44161 gcttgcgcgg tgttgagaaa gctgcgatcc gatttcttct ccagcagcgg caataacgcg
    44221 cgaaggacat cccaacaatg tccgacgaga ccgacctcga tcggataccg caagccgatg
    44281 cgagcggcat ccaaatcaat ctgaacggct cgcgcttggc ccggtttcgg ataaaactcc
    44341 agatacggaa agctggtgcc ggcgatgatg agggtatcgc aggcttcaag gacttcctga
    44401 gagggcgccg taccgaggag cccaatgccg ccggtggtca atgggtggtc gtccggcacc
    44461 acggccttcc caaggagcgg cttgacgatc ggcgcgccca gcaattcagc cagctgcagc
    44521 acttcggctc tcgcgcctaa gcagccccgt cctgcgagga tggcgatttt cgagccggca
    44581 ttgatgagcg ttgcggcctt ctcgagcagt gatcgggagg ggagggggag ggggtcgctg
    44641 tacaggtctc cgctatggcc gggaatgttg gccttggatc cgcgatcttc gacgctccag
    44701 tcctgaatat cttttgggat ggtgatatgg gccacggtcc gtcgtgaaat ggccgttttg
    44761 atcgcctcgt ccaccacatt accgacatga gccgggctca tgaccctctg gctataggcg
    44821 gcgacatccg agcagagccg ctccagatcg acatcctgtt gatagtgcgt gccgatcaga
    44881 tcgtggtacg tatgccccgt gatggcgagc accggctgtc cgtcacattt cgcatcgtac
    44941 aacccgttca gaaggtggat acctcccggt ccggaggttg cgagacacac gcctaatcgt
    45001 ctggtgaact tggcataccc gcaggccgcg aaagcggccg attcttcatg ccggaccaac
    45061 accaccctga ttttgtcctg gcgggttcgc aaggcctcat agatgccgtt gatgccatcg
    45121 ccggggagac tgaagatggt atcgactccc cattgaatca agcgttcgac caagagatca
    45181 gccaccgtgg ttgccatcgt cgttcctttc acggatcaaa aagaaggagg cgggctcgcg
    45241 gcgttgtgtt ccaaccgcgg tagcaccaat tcctggacga ggattttgag acatgcggca
    45301 atgggaatcg ccagaagcag accgtaaaat ccacccagtg ctccgccgat aaacaccacg
    45361 atcagcacgg tcaccgcgtt catttccatg gattggcttt gcacccaggg cgtcagcaac
    45421 caactttcga taaactgcac cacgaggtag gccacagacg gccacaccac caccgtcatg
    45481 acatcgaagg agccagcgcc ggcggaaaac ccgtccagat atttcaacag cacggccaat
    45541 ggccagccga tcaatgatgc atagggaatg atcgtgagaa tgccggtgat caggccgagg
    45601 ataaaccaat agcggatgcc ggtcagcgcc catccgaccg agtagaggac ggcagaggcg
    45661 agggcgatcg tgagtcggcc gcggaaaaat ccactcacgg cctggtccat cctccgcacg
    45721 atatgacgcg ctcgcggccg atacccggga gggatatacc ggcggagttg tgcgagggcg
    45781 ctgtcaaact gccaggcgaa aaaaaagaaa tagatcggta tcagcaccag tgccagggtg
    45841 acgtccgccg tggttccgat gacgctgcca agcactccga aggcctgccc cgtcccggaa
    45901 aaaatcggtt tcaggatgga aagagggtcg tcccgaagac tcgtggcaat cgacgagagt
    45961 ggttcggaaa aattccccag ggtgacatgg tactgcgtgg cgatactctg gagatacccg
    46021 ggtacccgtt cggcaaacga ttgtacctgc tcggccaata gcggtcccag ccatgtcacc
    46081 agccctccca gcatcatcgc tgaaaggagg aggacgatgg acacggtcac gggccgtggg
    46141 atatgccaag cggtctcagc gcgccggacc agaggattca ccaagtaggc gagcagcaac
    46201 gcaaccagca cgggggtaaa cacgccgcgc aggtaatagc cgaaccagag caggaacagg
    46261 atgccaccca accagaggag atcccggacc ggcgtaattt cccacaaatg gcgattcgcc
    46321 acagaagtgc cccgttcctt agacgtctgc actcgcggtc tctcctccgc aagcgtctcg
    46381 acatagcctg gtttcatgag gcgttcgatc ctttctctca ttcgtcgaaa cggtaggggc
    46441 taagctgtgt gacttgattg ttccgagaac gaccgcagcg ccaacgctgc acgctcaatc
    46501 tgggcgttga tttcgcctcc gagcaagatc accacacccg tcaaatagag ccacagcatg
    46561 agcacgatga cccccgcaat ggacccatac gcagcgttgt agttgccgaa atgatccaca
    46621 taggctttaa agcctaatga cagccccatc cagagcagta cggcgacgac agaccccggc
    46681 gtcacccagc gccagtcatg atcgacgtcg ggacagacgg cataaatgat ggcgagggcg
    46741 agcagcatca acccgatcgc cacaggccac tgcagcacat tccaggtcaa gagaaacagc
    46801 catcccaatc ctatgaggtc ggcaatccat tcgccgatcc gctcgccgta gaggacaagg
    46861 gtgatggaga tgatgataaa tgctgcgaga ccgatcgtga gtagtgccgc caccagccga
    46921 actttccagt aaggccgggt ttcctgcgcg ccatacacca cattgagcga ttccatgatg
    46981 gccgtgactc cgctcgagga cgcccagagc gctcccagga gtccaagcga aaggatgtca
    47041 ctcccggagc ctttcacgac actatccagg tacttttgca gcaatgataa ggcgtccgca
    47101 ggtaacacgg tccggagata ggtcatcaac tccggcagca cctgttccaa cgggaacagg
    47161 cccagcaatg cagtcaggaa cagcagcgcc ggaaaaagag ccaggagaaa gtaatacgcc
    47221 aattgagccg cacggccgag gatttcatcc ttctggctgt cggtccaaag ccgttgccca
    47281 agctctttcc atcccagtcc gccgagtttc cagggattcc atcgtgggga tgcactggtc
    47341 agcgaggccg acaccctgac tcctctactt catcggaact gtgatgccga tccggccgaa
    47401 gacctggcgt tgccgcgtcc ttcagtctct gaatacgaag cctggtcgta cgacggtcgg
    47461 gactgcttcc cttgggaggt cagcgtcacg ccgagagacc gggcgatttc gggaatcagc
    47521 ttgtttgcga attcgaccat gagcgtacgc ccggcttgca ttaatgcggc cttggcctgc
    47581 tccgtatcga tctccaattc acggctcatg gtttcccaga tagtggagtg acttcgctgt
    47641 tccgttctgc gggccctgtg ggtgggatcg ggatagtagt cgtaaatgcc ttcggctcga
    47701 cgagaagcgg ttttgccttc cgtctccgac tccggcatga cctgtgatgc gtgagcgccc
    47761 ggcggataat aggggtacac cccgcgttgt gcacgcgtac gggcttccaa cagaccgatc
    47821 gcgtatccgg cgcaaatcgc tccgcccagc atcagccatg ggtagtcatc gaccttatgg
    47881 atggggtcca aggcggcttt ggttttgtcc accatctgcg tgacgctctg ggatccttcg
    47941 tcgagtaagc gcgaaaattt catcgtggct ccctccgccg tgtctttgat ccgttgttcc
    48001 aacagctcga gcttttcggc gatatccatt ctcgtctcca caatatcttt tatgtcttgg
    48061 tcgatagcag ctgttctttg atccatttgg catcttcctt taatgagtaa aaggtgcgaa
    48121 acggccagag ccgaagagtg gtgccgatgg cgatcagacg agcaaagaag gccgcgcctg
    48181 cggcagccgc aacgagtgcc acgattccat aacaggccca cagtgggagg ccgctgtaaa
    48241 agtgcaacag gtggatcaac gtcatcagga gaaataatac agccaggaag gccagcatca
    48301 tggcgatgcc ggcgtgcatg gcgcttttga cgaccttccc caactcgtgc tgcatttcat
    48361 gccgggccag ttggacctcc tgggcaaaca atcgccgaag atcctccact aaccctgtga
    48421 ccagcgaggc gatcgaaatc ggatggggat tttccatata gccgtcctct tccggttaat
    48481 cggttcgagc gcgggagagc agatatccgc atcccaggcc gagcagcagt gtttggagcg
    48541 ggtaacgtcg aatgagaatt tcaaggtcct cgacgacccc gccgatgccc tgttcctgta
    48601 agtagacaga ggtttctttg accttgcgca cgactgtgtc cgccgcctcg cctgcagcgc
    48661 tgtctttggg aagacggtca cggaggtggt ccgcagccga ttccactgcg tcaccggcgc
    48721 gtttcgccat gtgttctgcc gaagtggcgg tgtcgtggat ggcatcttgt attgacgagc
    48781 cggcctgttt cgcccggtct gccatatgct tcatatcctg ttccatcggc cctcctcctc
    48841 actcgtgtat agggtgcatg ttgagcagat acttgcccag aatagcggcg tgaccatgat
    48901 gtttgaactg ggcagagccc tagtcagcca ggagtctcgc gccagcgatc gcaccgacat
    48961 taaagagtcg gcgactccgt atgcacaccc tgaggttttg acacaggcgg gcggtgcatc
    49021 cgaagcgtga gatcgggacg aaggcgtttg gcaggcagcc gtagggtgat cgattgtccg
    49081 cgctgggcgg accggtggag cgcttcaata atctggacgt cgaggagacc ttccgttcca
    49141 ttcggttccg gctgtcgatc atgtgtgaca cagcgcgcaa aatagatcag ttgtggcgcg
    49201 aattgatcgc ccggtgtaaa cgtacgtacg ctcgtgcgcc ctccgatcgt gacggaggct
    49261 ttcaatccac cgacgtactc atacgccggc tcgacgcgta cttttccctt ggtgccgacc
    49321 acctcatagg tcgcggcgtc ggcggcgccg aaactacagg tgaaggtcgc gaggcgatct
    49381 cgagaaaatc gcatgatcgc tccggccgat tcttcgacgg attgaaaccg gtgatccggt
    49441 cctcgaccgg cgagggcggt gacttctgtg ggctcatccc gaaatagata ccgggccgcg
    49501 ttgatacagt agactccaat gtcatacagg ggaccgccgc cgagctgttg ccgcaagcgg
    49561 atatcgcctg cccggacctg ttgtgtgaac accgaatgaa aaaggcgcgg gtcgcccaac
    49621 cgtcccgact gtacgaggtc gattgtgaag aggttgcagg cttcaaaatg caggcggtag
    49681 gcaatcatga gtttaacgcg atgccgttcc gcagcctgta tcatgcggcg gcattcagcc
    49741 gccgtgacgg ccatcggttt ttcgcaaagg atatggatgc cggctttcgc ggctcgtacg
    49801 gcatattcgc aatgcaggct gttggggaga gcgatataga tcgcgtcgat ctcgccgcta
    49861 cgcagacatg cgtcgaagtc tttgtacggg taggtatggg tcacgccgta gcgtttggcg
    49921 aggacacgac gcttaaccgg atcgtccgag acgagtgcgg taagggtggc ggcagatccc
    49981 gtatgagaaa aagccggtag gaccgccatc tgggaaatgt ggccgagtcc tacgactgcc
    50041 acacgcaacg gtgaatgact cacctcctac tactcctttg tgaacatgaa aaaatgtgcc
    50101 ggcggagagt cggccccaaa cgcttcgcat ggcaaggccg ggtcacccac gcggtgcggt
    50161 cgtcatggca tcgagcagag gaatgagatc acgctcttcg ccatgaaaat ggacacgcac
    50221 agtctgcatc atatcttcaa agaactgatc catggattca tcatcgtccg tctccgcgtg
    50281 gcgcaattca tggatcatgg ctttgacttc ctcatggtca agcagaacgc gccggatcac
    50341 tggagtggcg tgttcctgga ggcggctgtc ttcctcgaac aatctttctt cccagtcgaa
    50401 gtgatcatcc agccgctgaa gaatatcgtc gacaatggac tgtcgggaat ccgactccgt
    50461 accgagatat acctggaata gggccagtat atggcggtgg tcattcttca taattttgat
    50521 gatgtcggcg gtctcgtgca gcgtcggtct ctgttccata gatccgcctc cgcgtggcat
    50581 atcgtgagcg ccggccgtta ccggacgtcc tgtcgaagca gagcgcgggg actctgttct
    50641 tcaagcgtga cctgtccggt cgcgttggcc gtgccttcca gcatgatggt ggccgtgatg
    50701 ccctgagcag aggatcggac atgatccggc tgctggcgcg taatgagaat gtccattgtg
    50761 gtgccgcccg atcgatgggt accggtcacc gtctgcttca agcgatccac ttgaggagag
    50821 atgtcgacgg tcatcgcttg ccgcaacttg ccgttcaatt cacggtcgac cagctgccag
    50881 tagccgatgc ggatggaatc gatcgcggct ttggactcat tggccatttg gagatccgga
    50941 acagaaatac tgcgtccccc ttcttcgaga cgaggagtcc cccagtaata gatcgtgcct
    51001 ttgagatcgc cactcatgtc gatcgccacc agcgcgcggc cgttggcatc cgaggccgag
    51061 acccgttcga tgacggcggt atcggaagcg gtatccaaaa acaccgattg gtgaaagagt
    51121 ttactctgca actgatgcga gatttgctga taagacagcg gtatccgcgc tacgagcgta
    51181 taaggctttc cttcgggtgg aatggatcct gaattgacca gggccactgt cggtgtggct
    51241 tccgtacagg tcgcttcata ggtaatgaac ggcatctctt tcgctgttcc cgaaatgact
    51301 gtctcctgcg tggtcccttt ctggccgccg agaatcattt cccgaggatt gccatacata
    51361 caagcccggg tgttgagaga cgcaatgggg atcgataatg ggccgcccaa atcatcccag
    51421 attcgttgca gtggaatccc gaactccttg ggatgtaccg cttcagccaa ggccgtttta
    51481 atctccgaca tgccgaacag atcgggaagc tgttcggaaa tcgggacatt gaacatattg
    51541 cagcgcgcat cgctatcagg tttggtggtg acggctgtct gtgccggttc gaagccgaaa
    51601 gatagagcat cgccgttctg aatcatcgtc aaccggcctg tggcgtcgat tgaggtgtat
    51661 aacgggtcga gcctgcaggc acgtgaactg ccacgcgatt cgatatcacc tttatattcg
    51721 gcatggatcg cgaccagccc atcttgaatg tgaacatcag gctcaccaac cctcacaaaa
    51781 gtccacctga actcctgccc cagggggtgg ccggtctccg tgatcctttc gggaatggcc
    51841 ttgcggatcg cggtctggat gggggtcagg tctgttttga tttgaacggg cacctgggac
    51901 acaggcgccc gggaatcctc tttggtctgc gctgggaatg tgggcacggc actggtcaga
    51961 ggaggcggga ccggcttgga tggcgatttg ggtatggaat ggctgcaggc tcccaccaac
    52021 agggcagaaa agagcacagg cagtatatgt gagacatggc gcgtcataat cagcctccta
    52081 gacctcatca cagcagcgat cgtgcgatcg ccgcctcccc cagaagatgc tcatcctggt
    52141 ttcttacggc gcttgaattt gtaagttgtt gttcacggtg cgcacaccgg ccaccccgcg
    52201 agccagttca acgacccgca ttttttgttc ggcggatttg accaccccgt tcagggtcac
    52261 gacgccgcgc tccgtgtcga catcgattcg cgaaaagttc gacagccggt cttgtgacag
    52321 tttggcctga accgctgtgg tgatggaggc atcgtcgatg gtctgaccgg cagtcttgcc
    52381 ggtggtggac tggcaggcca ccagggtcat catggcaaag gcgaacatga ggtgcgtgag
    52441 tagtttcatc atgatagcgc atccttttca acgtagtgtg acagcatcac tgactctcta
    52501 gcgcggctct tctcagcctc gccgtgatgt cgcttcttcc gccgtgttct gggcagtctg
    52561 catcgtctca tccacgaact cacgagcggc tcgccctgcc tttcgtaacg tttgtttgcc
    52621 accctcgacg tattcctgcc ctcgttccat ggcggagtcc caggctgcgc gtccctgttc
    52681 agtggcttca tccagttcgt ccttcgccct ggaggcatag tcacgcagag tcgagcgaag
    52741 ttcattgcct gattgaggag caaggagcaa cgcgattccc gcgccgatca atgcgcccgc
    52801 catgaatgcc gaccagttgg aagaatcatc cgtatgcatg tgcgacctcc ttcaacggta
    52861 ccgatgaatg gtgatgtgta gtgttcccgc gacgtccgaa cagatatctt tcgcgtgatt
    52921 tcagactagc aggatgtggc gatcctcaaa ctaggcatgg ccctagttaa ggctacggag
    52981 ctgctctggc ggtcagaacg atgtggcagc gtcgagactc agaagagcat aaaggactca
    53041 catccattga tcgttgcccc ggtagccgct cgcgagtcat agtgagatgc ggtggttccg
    53101 tctgaatgct catgcgtgtc attgctgcca tcacaagctc gcaggtccag gcgtgggtac
    53161 gtattatcct cctcatggcg tttgtgtgca tgatgaccat agggtgcgtc tacactgctc
    53221 cttttacgga cgccgaatgc cggactgttc ctcacagtat tgcgacgatg gaacgaatag
    53281 ccctgggcgg cctgcctcag agcatatggt ttcgcggtgt ctctgattcc
//
LOCUS       DAOQDS010000024        52942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_200_length_52942_cov_20.607938, whole genome shotgun sequence.
ACCESSION   DAOQDS010000024 DAOQDS010000000
VERSION     DAOQDS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 52942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52942
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200_length_52942_cov_20.607938"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PK589_07675"
     CDS             <1..193
                     /locus_tag="PK589_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19519.1"
                     /translation="QFRVQRSCGGMPDGWIEVLREGAAAPSFLLADRARSECARPMRA
                     VKADAATPKGNAGGKWREH"
     gene            472..3903
                     /locus_tag="PK589_07680"
     CDS             472..3903
                     /locus_tag="PK589_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19520.1"
                     /translation="MAELYETAVTLLEEQPLPGRSRIIAHCVREIANALPALLAGVER
                     TRLEYDKRLDKIALARHRLTRLPIQAPRSMGGTSISDPQDALTQEFFNQIEELIKEHK
                     ATRLKRDTIAERLFQANRPENRHLGDALRPVLRRWTDLKDWFVGHAHDNGKTDAQCDW
                     SDIKRRFVLFERTVLTLGQGFFATIADLDELIRTSTPADVHLVVTQLGHIEHYKYFFE
                     HLDDPAWIPALRAEGIFNAPPSVEPNAIPRWAASEFLVRVIDRGDPTEGMKALSQIAD
                     ALTQQSLVNPLVIRDVVDVLRKVPAEHSVSFMPRLKQWAHHDRGFLFWRQLGKLVTHL
                     AEGGKPHEALTLLTPLLAIEGVRADDDFEYSYKPVKPRISMYEYIQIVDEHLRPVLER
                     RGEELFDVLCNILQKAAKLSRRSHSSEEWDDNSRVWQPDLSIQDEDDRDDVRSVLVVR
                     IRDAAETLLNDGRVDLGPLVEDLEARRWLIFRRLALYCLVTTASAPRDLIAPRLTHEA
                     SFRDRNLGKEYDSLLHIGFTRLTPDEQQTILSWVEAGPENIEQLIRNYETSTGRRATQ
                     EDIEKHHRSWQWKRLWPCHTALPATWRERYATLVEEFGEIEAPLPTTREARDVLEGER
                     APRPEQDLRTLTPAALREYLLAWTPERTTFPYPTRSGLAEVLTSLVTADPVAYANATE
                     EWLHLDPTFLHGIIRGFTKALQVDHPFSWAPVLSLCRWILAEPRTIPGRSVDRWEADL
                     DWGGTRWWIVELLRVGFQDETHGIPIDLRDVAWQLLETLTMDPDPEADEDEEDFGRPS
                     QSMNRAISSVRGRAIEALMFYPGWIKRQTGDAGPPRLPAEARAVLDRHLDLTEDPSLA
                     IRSLYGRWLPWILVFARDWGTASIPRIFPEYSQRYWLAAWEGFISFNNPYPEIFEPLQ
                     PVYAQAIAQLGSPSAEADDTRDQRDERLAGHLMTYYWRGHYPIEDETGLPYQFFARAS
                     DQVRAEAIEFIGRSLDRTDDPVESVVLDRLQRLWAQRFDGARQDPATHQAELRAFGWC
                     FGSGKFEPRWAVQNLLDVLRLTQSIDPDFQVLERLPDHAGAFPLEVLECAKRLIEGQT
                     SHVELSSWGEDLRRIFSQTRNHPAPEVRQASDDVIEVLGRFGHLGYADLLSRESG"
     gene            4045..4335
                     /locus_tag="PK589_07685"
     CDS             4045..4335
                     /locus_tag="PK589_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19521.1"
                     /translation="MALLLAFYLIFSAVIPASALEGAQATAAMVIKADVLYWEEGELV
                     VKEFSGHEERLKVTPETKIEGVVAGRLKTGDKIVAQITDDRKALSIKVQIPE"
     gene            complement(4466..5185)
                     /locus_tag="PK589_07690"
     CDS             complement(4466..5185)
                     /locus_tag="PK589_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19522.1"
                     /translation="MKPLRIITLLMLSLAVIMGVQCLTAEPAAAHNWLGDCVVDFDNT
                     FALTYIYGQARGNFGYPTGIDNTGELQPCNATHQACWTYRHRCFGNYINVEDMTYGHI
                     HLTFDSPSLLTPCFVDPGDGPGAGFGRTVGDNCVAADWIHEPRTLGTHGKDHWIKIWM
                     EDRVTHQPRVFDMPIIRVTGTEPIQFWFKKSNGSWWYWSNLAPNPWLVGGWVQDVVEV
                     RIRGAQAAKGPYFIGAFSILD"
     gene            complement(5240..5641)
                     /locus_tag="PK589_07695"
     CDS             complement(5240..5641)
                     /locus_tag="PK589_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19523.1"
                     /translation="MSSVSYRSLLAPAIPYVIVIPATILSGCVPPPPHQFVPGGPVGE
                     AGQTVPGPRQASDDEVRAMLAESSRHQSMPSERVDLNGLLTQISQATTFRQKQHLVDQ
                     YHHAALLLPEAERNQAFQRLRDLGNHGRTTP"
     gene            complement(6027..6155)
                     /locus_tag="PK589_07700"
     CDS             complement(6027..6155)
                     /locus_tag="PK589_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19524.1"
                     /translation="MHDAALWASWYVWLLAVPVLVIGTLIRDWLHRRHPVRLRKRS"
     gene            complement(6382..7293)
                     /locus_tag="PK589_07705"
     CDS             complement(6382..7293)
                     /locus_tag="PK589_07705"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNJ19525.1"
                     /translation="MPATLQAFIAIFVLGALLRVSGLLGKIHAERLASFVFSISLPAT
                     ILVSLDHVTFTATAWRLPLAACLITVSMLLCAWQLARRLHLTRPTQGGLLLGTGCINS
                     VYFAYPVVLATFGDQGLARAILFDLGQTTLTLTLIYALAVGHGTKSTTARSAIVRFLS
                     SPPLWALCGSLLLSLFGLHLHDWLRQILTPLHLTTTPLASLVLGLSISVAVTHRTFAL
                     ATLGVVMRMAGGLLLGLGVAFVLNLTGIERAIVVLIAAMPSAVTAVIFAADTGLDEDL
                     VTSIVALSICVGIATLPWLPQLIRLLE"
     gene            7404..8630
                     /locus_tag="PK589_07710"
     CDS             7404..8630
                     /locus_tag="PK589_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HNJ19526.1"
                     /translation="MNQAQPSVPPRQAAVFFILVTVVLDMLSFGIIIPVLPKLVEEFL
                     GGDTAQAAEIYGLMGTSWALMQFVCSPIQGALSDRFGRRPVVLLSNLGLGLDFILMAM
                     APSLAWLFGGRVISGIASSSFSTAGAYIADVTAPEKRAAAFGLMGASFGLGFVLGPAV
                     GGLLGALDPRWPFWGAAATSLLNACYGFFVLPESLPVEKRAPFRWQRANPAGALILLR
                     SHHELFGLATANFLMNLAHGVLPSVAVLYLGYRYGWGPSAVGFTLAAVGICAMIVQGT
                     LVRPITAKLGERRTLLTGLLCGATGFAIYGLAPTPLIYCLGIPVMAFWGLAGPSAQVF
                     MTRRVSASEQGQLQGAIASLTGIAGLIGPTLFTQTFALFIGPQADWHMPGAPYLLSTM
                     LLLVSAATAWRATRPV"
     gene            complement(8690..9640)
                     /locus_tag="PK589_07715"
     CDS             complement(8690..9640)
                     /locus_tag="PK589_07715"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ19527.1"
                     /translation="MIGFWFLLLWCLPAQAWAVSPGDVTPTTSPNQLSGRELLRIGEV
                     HDKQEHWPETLTYYQLALSKFREKKQQQGIATTLMKIARVHERQGKLQDAHASLREAE
                     QLFAQAADRSAHAEALLAMGRVAAQLGQRDESRDALTKAAALFTRVRNARGWNETMVQ
                     LGLLQVVEGSADAGLSSLQQAAEEAHTRRHVDQQFTATVALGDAHWLLGRLEEARTHY
                     LDALALAQAEHHLPFEGMLHLRLARLDQGKDTLNDRVALGKRAIQIAQAIHDTAAEAE
                     AWSLLADLHRQLGQQAEADTAEMRALALYRSRELFVHGVQ"
     gene            9895..10470
                     /locus_tag="PK589_07720"
     CDS             9895..10470
                     /locus_tag="PK589_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ19528.1"
                     /translation="MAGATGTRQKKQAPQRKKAGPVKKTTRARAGQPSGAVIVLSGAT
                     PLRRQKAAEVLAEELQLHLAAVDLSAVVSKYARETEKNMTRVFDMANRHGSILFFDEA
                     DALFGKRTQVNDSHDRYANSEVSYLLQCIETHQGLVILATNGKSRIEDAFSPEKPVIV
                     LGGTAKKQAGVKPKPKAKSTPAPTKKVRKKK"
     gene            10571..11020
                     /locus_tag="PK589_07725"
     CDS             10571..11020
                     /locus_tag="PK589_07725"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNJ19529.1"
                     /translation="MDLPIRSPRQKLGEYVILPRLIDKVRLHAAGALPLDYVRNLLKP
                     GLTLDGRFLKFTGLAADALEGAILAAESDSLVLAWVDEQAIVHSDAEKQAWSEKAAAF
                     HPDPRVAKYLMMSLPDLATSLDITRYTLFDLIDMDEGRLPVPSRPKA"
     gene            complement(11056..11307)
                     /locus_tag="PK589_07730"
     CDS             complement(11056..11307)
                     /locus_tag="PK589_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNJ19530.1"
                     /translation="MTWIVYILECADGSLYTGITNDLERRMRSHASGNGAKYTRHRGP
                     FTVRYTESLASKGAALQREAAIKALDRAAKVALITALQC"
     gene            complement(11399..11557)
                     /locus_tag="PK589_07735"
     CDS             complement(11399..11557)
                     /locus_tag="PK589_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19531.1"
                     /translation="MMTQRLRKSSMYRFHDSVRHDRKYLIVALAAALILAAILASLGL
                     TVFPTFPK"
     gene            11816..12706
                     /locus_tag="PK589_07740"
     CDS             11816..12706
                     /locus_tag="PK589_07740"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HNJ19532.1"
                     /translation="MSLPSRIALELFTAKQAWSAGNEGKARVCARRAVAAAAESWFAH
                     RGEQVAQADAMTYLRRIQQQGTFPLPVRQAAERLTTAVTRRHEAPFTTDPFTDASLII
                     SHLAADSSSEGHADVSPEREDDLQSRPGAGRILIILIMGVSGAGKTTVGRRLAEVLGW
                     RFLEGDDLHPIANIEKMRSGHPLTDADRRPWLERLHTAIADQVRMNQPAVMACSVLKA
                     RYRTVVEEGFGHALRLVYLKGTADVFRERLAGRRNHFMRPELLDSQLAILEEPADALV
                     VDAALPPDEIILRIRQGLAV"
     gene            complement(12743..13102)
                     /locus_tag="PK589_07745"
     CDS             complement(12743..13102)
                     /locus_tag="PK589_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ19533.1"
                     /translation="MNEQFARSLAEAWIDAWNRHDLEAILHHYASDVEFTSPFVPALS
                     GDPSGTLHGRDRLSAYFQKGLHTYPDLHFDLLQVLTGIDSLLLYYRSVKGLLAGEMMT
                     VNAEGLIQTVRVHYVKE"
     gene            13206..13676
                     /locus_tag="PK589_07750"
     CDS             13206..13676
                     /locus_tag="PK589_07750"
                     /inference="COORDINATES: protein motif:HMM:NF021425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HNJ19534.1"
                     /translation="MLVSAPISQQTSLAPIALFKLVKGLLVLLAGSGFLRWIDPEIDS
                     GLSPVLDALHLNIHTRLLHALAITVDSLPRHSLLLMSLVSLGYAALLFVEGFGLWSET
                     SWAAYLTVVSTCVLLPGEFHAVVREWSMAGLAVLAVNIAIVGYLVRRLADETLR"
     gene            13783..14175
                     /locus_tag="PK589_07755"
     CDS             13783..14175
                     /locus_tag="PK589_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19535.1"
                     /translation="MVEVNSMYQPRMRNDDIKAQIDKLEGLAHVTAVHPTFDTFDEET
                     ETILTRIFGGSHPYVESYKYATLGEAEAIVNLPESAQEPLTRDTPKKGLQQRRQVLQS
                     ILTDLQGLEAEEAEALTGEDHEDPPGPS"
     gene            complement(14330..15142)
                     /locus_tag="PK589_07760"
     CDS             complement(14330..15142)
                     /locus_tag="PK589_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ19536.1"
                     /translation="MPAFLTATARSGRLGIASVGGLCLAISLTAGPLLWAGSSSPTKP
                     GKGPQEAEQGRVLFNGKGLCSTCHGVDGHKGQLPPHLTQHIRDNIAHLDPAPADLRQA
                     RTLTLTTDKQRFEIIRHGHLRTAMYPISKDTISDEEILALLPYLASIRGAVSPPASVP
                     ASSFSLRGDANNGRQLYHELGGCAICHGIEGHLDSRPPMSADLISRLKALPHPPADLR
                     HPSSLTAQSDEDLYRSIKLGHPGTAMYPKTLLRDEDIRDLIAYLAVLRGGSQ"
     gene            15292..16032
                     /locus_tag="PK589_07765"
     CDS             15292..16032
                     /locus_tag="PK589_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNJ19537.1"
                     /translation="MGSMIHTAMNMVDHRHCLALSGCFRGKLCEQLMNRPGRHMPKGS
                     RVYGPGDPAQSVFYLRRGFVKLTSLTEDGRELILRLHQTGEVFGELCHCTGERREQAI
                     AMEDSEIVELSFDEFIAHLQSNRPAFLLFLSNVAQQLSAAYDQIQTLSFNSTMERLVR
                     TLGRLADEFGEPDGEWIRLTHYFRQDDLAQMIGARREVVSTLLNQLREQGLITYARKD
                     GVLLRRAELDRFLGSTAQSSASLADSGF"
     gene            16166..16816
                     /locus_tag="PK589_07770"
     CDS             16166..16816
                     /locus_tag="PK589_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19538.1"
                     /translation="MRKVIHACSVLMLSVATVACAHTTDTPAPVASSAAGHQAKVAET
                     KAALRDLWLGHILSIRNVAVATMDKNAAARSAAEADVVANAQQIARSIEPFYGKPAAD
                     QLFTLLAGHYQGIREHLDATVAGSTSQQDAAVKALTANAAEIATFLSGANPNLPKDTV
                     MGLLMAHAAHHLTQFQQLKDGEYAREAETWKGMKQHIYVVADALTQALAAQFPAKF"
     gene            17203..17787
                     /locus_tag="PK589_07775"
     CDS             17203..17787
                     /locus_tag="PK589_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ19539.1"
                     /translation="MGRIRHCVVSGKVLLLALVLSVAPAICQAAEWVHEGMADEGKMT
                     FGFRVGPSFMTQSSGLSTTGPALNFQGMYGINKWFRFGMMLEWENHSIDGPRNGSLNT
                     ITLLPVNLEYRPGHFGALIPYVTSGIGVNINTKDQADSFAWRFGAGLDYPMTSLFQGA
                     PKGLMLNMETAWKRNSADGDGSSMGLLFGVRHTF"
     gene            18097..18783
                     /locus_tag="PK589_07780"
     CDS             18097..18783
                     /locus_tag="PK589_07780"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HNJ19540.1"
                     /translation="MSKKEIDAARRLMSLMFKAHPWHGVSMGDQAPDLVTAYIEIVPS
                     DTVKYEVDKATGFLKVDRPQRFSNFCPVYYGLIPQTYCGEQVAKLFGARAGRPDMIGD
                     GDPLDICVLTEKAIPHSDILLTARPIGGFSMADGGEADDKIIAVMRDDAVYGTLTDLK
                     ECPMTLLDRLQHYFLTYKQAPGSTAHNKVEITSMYGRDEAIKVIHASHDDYKAKFPEL
                     EFMWPASMNS"
     gene            18813..20540
                     /locus_tag="PK589_07785"
     CDS             18813..20540
                     /locus_tag="PK589_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNJ19541.1"
                     /translation="MKHTTTPSPTEPAAKGFSPGFAALGLEASLLTTLEALGYEEPTP
                     IQREAIPPLLEGRDLLGQAATGTGKTAAFALPLLQRIAHGPRQRPTALVLVPTRELAV
                     QVSEAVQRYGKELRISVLALYGGQAMGPQLQALRRGVEVIVATPGRALDHLRRKTLKL
                     ADLQVVVLDEADEMLDMGFADDLDAILEETPATKQTALFSATMPPRIASIARRHLKNP
                     IEVTIAREPVKAGAAPRVQQTAYVVARQHKVSALARVLDIAMPKSALVFCRTRLEVDE
                     VTAALNSRGYRSEAIHGGMSQVQRDRVMQAFRTGQTELLVATDVAARGLDIPSVSHVI
                     NYDLPSSLEVYVHRIGRTGRAGREGAAMTIVEPREQRLLRAVEQHTKARITIAPVPSL
                     GDLLAKRLERTKSSIQETLEAGGLEDFRRVVDALSASADPADIAAAAIKLVYRSHGGD
                     REEEEIPAVQMRAPEPYRSSRPAYGSAPQGDRGRAPRGGPSRGGRAAGTVRVYVGAGR
                     AAGIRPGDLVGAIANEAGVPSRMIGAIEVEERFSLVDVQEEAARQIIEALGRTRIKGQ
                     KVAVRLFRD"
     gene            20731..22143
                     /locus_tag="PK589_07790"
     CDS             20731..22143
                     /locus_tag="PK589_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ19542.1"
                     /translation="MDRLPWSRWHWLVVTALGVTWILDGLEVSIVAALGPVLTHATTL
                     HLTASEVGLTASAYLAGSVVGAIVFSYLTDRQGRKKWFMFTLMLYLAATVMTAFSWNL
                     WSFMFFRFLTGAGIGGEYAAINSAIDELIPARSRGHTDLSINGSWWLGSAAGALLTIV
                     LLNPLLIPEYLGWRLCFALGAVLGVSILLVRRVVPESPRWLMTHGSVQEAERIVSGIE
                     AQIMRDEQLRALPAAEGSITIHGRTPATLATVARELFTAYPRRTSLGIVLMVTQSFMY
                     NAISFTYPFVLTKFYAVPSHSIGLYIVPFAIGNFLGPLLLGRFFDTIGRKQMISLTYA
                     VAGCLLASTGYLFFQGAFTSVTQTAAWSAIFFFASAGASAAYLTVSEIFPMEIRAMAI
                     AFFFVVAQGAGIAAPWLYGKLIETSAESVMYGYLLGAGMMVIGAIVELWLGVKAEGRS
                     LEHIAMPLSAQPVSSHAPHV"
     gene            22275..23831
                     /locus_tag="PK589_07795"
     CDS             22275..23831
                     /locus_tag="PK589_07795"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNJ19543.1"
                     /translation="MNVRPWTAALRDWQARAVADVLAQKREDYLVTATPAAGKTRFAL
                     RIAHHYLAERAASRVLVVCPTNHLRTQWASAAGQAGIQLDPALSNEQACEARDYHGAV
                     VTYQQVCLAPDVFRRACRNRKTLVILDELHHAGDGKDWGKALRESFGEAVFRLALSGT
                     PFRSDNNPIPYVRYEQGESQADFTYGYSHSIRDGVCRPILFPSYEGELTWLSDGREHR
                     ATFEDGLTFERQRERLKTALLQETWLGPVLSDAHAELQRLRKQEQADAGGLIVTMNQD
                     HARQVADLVHRLTGTRALVAVSDDPSASKTIETFAHHKTQQWLVAVNMVSEGVDIPRL
                     RVGVYATNVLTEMYFRQVVGRFVRMQDKVPKPQRAWLYLPKDATLVHYAKRIKVERDH
                     VLEEIMPAVQRTLFGTAATSLKEYIPLNGVARLDALIGADEGEPSPAGKGHPEPAVAL
                     HDQKNQLRDKHRLLVGAVARKCGMDHRQLNAELIKRTGGRVDQATIPQLERRIALLEK
                     WRDSGFDKKR"
     gene            23914..24483
                     /locus_tag="PK589_07800"
     CDS             23914..24483
                     /locus_tag="PK589_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ19544.1"
                     /translation="MTGKKGKRPVSAAAKISRKKVGMKVVKKAAPRKQAPARRAKVTR
                     TNKSSVAVVVLSGSTPLRRNKAAELVAEELQLDLSKVNLSSVVSQRVGETEKNINRVF
                     DDAERSGSILFFDGADALFGKHRPAKDGASHYANAGAAHLLQRIEDHNGLVILTTNGK
                     SRIDQALSKQARVSVMVGIEKKKRKQKTA"
     gene            complement(24654..24824)
                     /locus_tag="PK589_07805"
     CDS             complement(24654..24824)
                     /locus_tag="PK589_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19545.1"
                     /translation="MPKARSAKNCYYCEAEDRIKMTFMLCGLCHRHFCSAHGVPDLEQ
                     CTKCLEASEETE"
     gene            complement(25156..26784)
                     /locus_tag="PK589_07810"
     CDS             complement(25156..26784)
                     /locus_tag="PK589_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNJ19546.1"
                     /translation="MSLPVEKSASDELRREVLRRRTFAIISHPDAGKTTLTEKLLLYA
                     GAVHLAGAVQARSTQRQAKSDWMELEQARGISITSTMLQFDYQGARVNLLDTPGHQDF
                     SEDTYRTLMAVDSAVMVLDGAKGIEPQTKKLFAVCRKRGIPILTFINKMDQPGRHPFD
                     LLDEIERTLGMTAVPFNWPIGEGSGFQGVYDFQNRQVLFFQRTSHNQRRAPMTVEAFP
                     NPKLAETLGDAYGPLQEEIGLLTGAGTSFDRDRFLAGELTPVFFGSALTNFGVGPFLD
                     AFTKLAPPPGPRHSAQGLVQPTDETFSGFVFKIQANMDPQHRDRMAFLRICSGRFEKD
                     MMVHHARLGRKIRMTRPHRLFGRDRETIDEAYPGDVVGLVNPGLFTIGDTLTSDPSLT
                     FDPIPHFPPECFGLLRTQDISKHKQFQKGLKQLEEEGVMQIFYSPDHVRREPVLAAVG
                     ELQFDVVMSRLENEYGVKTTVERMPHSLARWIVGDAATISAVYWPSDTVRLVDQQGRA
                     VMLFTTEHLLAYCIKSNPSIKFLLREEVEQATAQ"
     gene            27096..27845
                     /locus_tag="PK589_07815"
     CDS             27096..27845
                     /locus_tag="PK589_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNJ19547.1"
                     /translation="MNVDDIISYGELVSAEKLMLQKGMNFGVGKDYSILLMSQRANAP
                     YRDVVDDATGILIYEGHDQPRTKDCPNPKTVDQPMTTPKGTLTENGRFFRAATDFKHG
                     LREKAELVKVYEKIAVGVWCYKGFFELFDAHLISRLEKRRVFQFFLKPVQKKAFGRIG
                     ELSHKRLIPTHVKVEVWKRDQGKCVQCGLQENLHYDHDIPYSKGGSSLSAQNVRILCA
                     KCNLQKSNRIMGVFPWLWVASSRLLELGWHP"
     gene            27914..28129
                     /locus_tag="PK589_07820"
     CDS             27914..28129
                     /locus_tag="PK589_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HNJ19548.1"
                     /translation="MLNHPRDPLHGITLEKIVTTLVERHGWAELGRRLPVRCFLNNPS
                     VKSSLTFLRKTQWARERVEGMYIAELE"
     gene            28305..30623
                     /locus_tag="PK589_07825"
     CDS             28305..30623
                     /locus_tag="PK589_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNJ19549.1"
                     /translation="MPIPLTLAFCFLLSLMQISAVSAQSTSSSTDKAVRATLETVGVK
                     LDQPAANGPAPDAAAADLQHKRAAAEKELSELTTPQAQRKGTAGNQVDGRTAERRSLL
                     QQLVQIYEQHLDALRNLEQMRQRVREAERQEKEWDGFSTPAPYSVLKIDELRDAVRSM
                     TLASQGAQTRLNMTESLTESTQRSLKASQEKARQLSERLEEVQDPAKREALTGDRDLA
                     RLRERVEGARAGMLEAEKKQIQEEVAEARHRMALLTRQRTVAEQDSAFTQQDYDKIKK
                     RLDAEHQSLTDEIERAVLEQSTQRQAVAAGEAALAAANAKDSSSKRNASRPKGDRLTA
                     LTESVELKRLQFDNANLHVELLREMLTGLEQERHIWDIRFATAQETLSVVEEREANAK
                     IAAAAKQIRGWKEYGSQQLSMVGNHISDVEDRLAEAPSAARAQVFTDKLRLYRHREDL
                     YRRALQRTDSLLGLIDNKQAEFAHREQARSVFARMKEWGRASVVMLGNAWHVELFAAE
                     DTIEVDGKNITGRRSVTVGKVVTALAILIVGYWVAGIMARFAERQAIARLQIDPNVAN
                     IIRQWALACFFLLLVIVTLMSVKIPITAFAFLGGALAIGVGFGTQNLLKNVISGLLLL
                     MERPLRVGDVIEVDGIRGMVTTIGLRSSTIRDINGVETLIPNSNLLEKNLTNWTYSSF
                     RKRYSLRIAVATGSDARHVKEKLRELAGEHGQILKEPEPYVLFEDFSEHALVFVLHYW
                     IEISPGIDAATVASDLRFMIERTFAEEKIARK"
     gene            30671..31222
                     /locus_tag="PK589_07830"
     CDS             30671..31222
                     /locus_tag="PK589_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNJ19550.1"
                     /translation="MAPNATICKAVLHIADVDRQYYEDHSITLARHPSETDERMMVRL
                     LAFARHAHEALSFGRGLSTEDEPALWQKDATGLIELWIEVGVPDEKTIRRACGRARQV
                     WVYTYGGRAADQWWKQHGAALDRLNNLAIVNLSLEGSRALTSLAQRSMDLHCTIQDGQ
                     VLIGDGTNAAQMDLLLWKEPARE"
     gene            31244..31636
                     /locus_tag="PK589_07835"
     CDS             31244..31636
                     /locus_tag="PK589_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNJ19551.1"
                     /translation="MKEKTRIPTDGEPVTWKSPFAALKDVVLPPAPPREQAKQLGTPA
                     PAPAKNRGRVDIIRQTAHRGGKTVTVVTGFVGISQTEKEQLAKEMQKACGVGGTVKEG
                     RIEIQGDRRDDIARILTKAGFRPVFAGG"
     gene            31643..32758
                     /locus_tag="PK589_07840"
     CDS             31643..32758
                     /locus_tag="PK589_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNJ19552.1"
                     /translation="MAEPLKNSFGADVPRTIARMIAAVSPRFDEKAFVRSVLDGYDEL
                     ELMPRAWKIAHVLRRHLPDDYERALEMLLASLDQQSKRKTASGMGAFLFLPHVFFVAE
                     YGLDHFEASMRAQYVLTQRFTAEFSIRRYLERHQAATLARLAEWTADPSEDVRRLVSE
                     GTRPRLPWAPRLRAFQADPRPVLALLERLKDDPSLYVRRSVANNLNDIGKDHSEVLVE
                     TARRWLENATAERRWIIRHALRSAVKRGEAGALHVLGFGHTPLVAVETIRIAPSRPRI
                     GSSVGVTWEVANQASGGQRVLLDLRVHYLKANGKHSPKVFKFKTVELAARETLRFSKS
                     LALTQLTTRTHYPGLHRIELLVNGHAYPLGVFHITAG"
     gene            complement(32775..33383)
                     /locus_tag="PK589_07845"
     CDS             complement(32775..33383)
                     /locus_tag="PK589_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HNJ19553.1"
                     /translation="MRVIVIGGTGTIGSAVVKRLSTRHEVVVVGHKKGAFQVDLASPD
                     SITSLFKAIGTCDAVVSTAGIAKFASLDDLTYDDYFIGLKNKLMGQANLVRIGRPFVT
                     NHGSFTLTSGVLSQNPMKGSASISMVNAGLEGFVRAAAIDLPRGLRVNVVSPPWVTET
                     LIARSMDPSIGIPADQVAQAYVASVEGSMTGQTLDPRQIAAT"
     gene            complement(33453..33767)
                     /locus_tag="PK589_07850"
     CDS             complement(33453..33767)
                     /locus_tag="PK589_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19554.1"
                     /translation="MEAAQANQVVELRLTYRYIKDHPWTVQAINGFLSAYFMEKPGFS
                     VQRHYDDLESGMHVWICDIPANMKITVLLRRLQADIPPCRHLQPAVEPSARPQYVIDS
                     PE"
     gene            33915..34751
                     /locus_tag="PK589_07855"
     CDS             33915..34751
                     /locus_tag="PK589_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ19555.1"
                     /translation="MLTAYNQVPVPSFMYGTAWKKDATTQLVQQAVAAGFTAIDTANQ
                     LIHYQEALVGEALLALAKQGTPRERLFVQTKFTPVNGQDHRTPYDAQADLTTQVKQSF
                     GSSLAHLHTDYLDSYVLHGPYARRGLSDADWEVWGAIESLYAAGTTRMIGISNVSADQ
                     LRLLCERATHKPMVVQNRCYAALGWDRDVREICRAHGIIYQGFSLLTANRDIFSDPDV
                     RTMAAKYGMGLAQLVFRFAMQMGMLPLTGTTDQQHMAEDLRSDQFTISTEDLQRLETV
                     GM"
     gene            34784..36400
                     /locus_tag="PK589_07860"
     CDS             34784..36400
                     /locus_tag="PK589_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HNJ19556.1"
                     /translation="MDSLHQLEIIILLLAVVLALTTIAQKISIPYPIFLVLGGLVLGV
                     VPGLPTVTLHPDLVFLVFLPPILWAAAYFTSLREFRQNLRPISLLAVGLVLATTAGVA
                     VVAHALLPGIGWAEAIALGAIVSPPDAVSATAIGKRLRIPRRVVTILEGESLVNDATA
                     LVLYRAAVGAVVSGSFLLGGTLLNFLFAALAALAIGLAAAWLTRWALCVTEDGYTQIG
                     VTLLVPYIAWVIAESVHASAVLACVAGGITLRQYFSGAVAPATRLQARAVWELLIFVL
                     NGVIFILIGLQLGALRDAVPAGQYGPVVFAGLMVSATAIVVRFLWVPLAALIPRCLSA
                     TLRARDPMPSWKGLFLISWTGMRGIVTLAAALALPMTTGTGAPFPFRSEIILISFAVI
                     LVTLVLQGLSLPPIIRWLRLEDEDDLDREQRLAREHAATAALARLDEVMTEDWVPREQ
                     IERVRLRYLRRLEHLNRTGLNEEERPEGSVETVQRLHHEVLTAERVALIGLRDDGTIS
                     DEVLHHLEQELDVAALHQGVGERRAGKGRI"
     gene            36402..36872
                     /locus_tag="PK589_07865"
     CDS             36402..36872
                     /locus_tag="PK589_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ19557.1"
                     /translation="MPIHIAAAQPVDAPIIAKLVGDLLAEIVAAIKTAAFGFSLAETE
                     ARARAWLEDGNYTVLLARDGEGAAGFIALAECRALYAEGRFGIIPELYVSPDRRSQGV
                     GGALVNEAKRVARSNGWARLEVTTPPLPQFDRTLAFYERQGFSVSGGRKMKVSV"
     gene            36869..37288
                     /locus_tag="PK589_07870"
     CDS             36869..37288
                     /locus_tag="PK589_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19558.1"
                     /translation="MIRPVVQEERTGCGIAAAAALAGVSYARAKAVAKSLGIVASDRK
                     LWSETEYVRDLVAQFGLRAARTRQPFHSWASLPDYALLAIKWHLEQGRPFWHWVVFVR
                     ENDRSYVVDSSPTLKAPLRTDFGRMRPVWFLTVTMKR"
     gene            37292..38530
                     /locus_tag="PK589_07875"
     CDS             37292..38530
                     /locus_tag="PK589_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNJ19559.1"
                     /translation="MNIAIIGGGPAGLMAAEVALAGGAQVALYDSMPSVGRKFLLAGK
                     GGLNLTHSDPSDLFLSRYGERRAQVAPWLAEFGPDALRTWARGLGIETYVGSSGRVFP
                     TDMKAAPLLRAWLRRLRQDGLTIHVRHRWCGWDEKKALRFETPHGATAVRADAVILAL
                     GGGSWPQLGSDGAWVPVLAGRSIAIAPLRPANCGFDVGWTEHFRTKFAGHPVKTVGVV
                     AKSPTGAVMRRMGEFVITDTGVEGGVIYAVAPFLRDEIEANGRGTLRLDLAPDRELPR
                     LIKDLSQPRGKKTMATHLQRRAHIDGVKAGLLREIVSKEDFADPTRLAAAIKALPLTL
                     TAPRPLHEAISTAGGVTCEALDKRLMLKALPGVFCAGEMVDWEAPTGGYLLTACFAGG
                     RTAGAGALAWLAERAERAKA"
     gene            38517..39074
                     /locus_tag="PK589_07880"
     CDS             38517..39074
                     /locus_tag="PK589_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNJ19560.1"
                     /translation="MPRHNVTIDGMALVVPGIGNSGPRHWQTLWEQQHPRWQRVQQRD
                     WDRPVLTEWLPALESALAGISAPAVLIAHSMGCLLVAHWAQQASVPVRAALLVAPPDP
                     RSPAFPASARGFETVPSGRLPFPTLLVASSNDPFGSVAYAKQCATVWGSAFAEAGAIG
                     HINAESEIGAWPAGLVLLERLLASS"
     gene            complement(39138..39920)
                     /locus_tag="PK589_07885"
     CDS             complement(39138..39920)
                     /locus_tag="PK589_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNJ19561.1"
                     /translation="MAGSLLRIACLHLAPIPGAIAQNRHLLTQAITRAACSGASWIIT
                     PELAVSGYTFADTLGTEWIEPQPDHWMSRICRLAARLGVTLFLSHPEKEPRSGQLYNT
                     VFAIGPDGRIAGSHRKINALRVGSEAWSTPGTDATAFRLAPFGRVGLLICADAYTPGI
                     AKSLKAQGATVLVSSAAWAPGLHGPNDEWERCTKDTGLPLFVCNRTGHDRTLDFRKAE
                     SVVAHNGKRLLSLSCEQSAIFLFDWNLTTGTLADRGHQRILI"
     gene            40064..>42218
                     /locus_tag="PK589_07890"
     CDS             40064..>42218
                     /locus_tag="PK589_07890"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNJ19562.1"
                     /translation="MNHPIVPPSRKRLGQWLLLLVLLLALLCVATILALPWLLNRPAS
                     MAALLQQVEARTGHRISFEQSHVEIFPSPRVTLMQPRLYAATSPDPLLFAERVEVALQ
                     WLPLLEGSLVVKDLVIDRPRVTLRRSPNGNWALGNEHSPGPDSEPTPPFALLQVVRNL
                     LVVQGMVTLVDESASPSTSLNIIVTQAALSSEMLGRRAKLHLSGELPQAGGRAAFLWE
                     GSITQQGDEDGIQAEGDLRLHQLNVRQVLSAWMGDGRITDGFSQPAQLTAHVRWKPGA
                     AGYDLLADDVRAELADVSLQGSGAMLGIGTAQARFSSSLSASPVTAARLLNEVPSAWM
                     SSALRAQFVDHAVEGLITLQSLSIAGDIAPGARPHINGLLAIRNGRFTLSSPYPPVEA
                     LTVSMVFDADQLRMTEVRAQCGPLRLAGRDLLITQWATDPHIDVKITGTAPVSGLVET
                     VRRLDEFPLLRDQVTDVEQPTGDVEMIAHVMGRPLSGQPLTLVDADLRLQRGGGRSAL
                     LPMPVRQVDAHVTVTPTMVAIEHLNGWFGPAAFQTHGSVTMADGKAYSNVTLAMSLEA
                     SELQSWWAEQAAEHFVPELDGSIRLRAAVTGALGHPRVKGRIDLAEAGVRWQNWLTKP
                     LQAPAAIDFEGQVSRDSHLLIRHVGITFPPVTVTASGTVDLDGEREFSAHVSSGRIAV
                     ARLPKGVVLGPVLAGTLEATLDMEGRMTDRASWRTS"
     assembly_gap    42219..42318
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <42319..43560
                     /locus_tag="PK589_07895"
     CDS             <42319..43560
                     /locus_tag="PK589_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HNJ19563.1"
                     /translation="ATLDMEGRMTDRASWRTSGQIRFDEGTIKVENLDEPIRDAFVTL
                     RFDRDRIHIPRMAFHVGASDLRISGSIAQWADQPKARLVVESSQIDLAAFEMSRRRPS
                     ASARQRSSDHLWTDTTLHAFLFADHVYYKKFLLTDLSTKITWDHGLLTVERISGDTNE
                     GQLAGQVKIRTKNGQMEQARSTFRASGIPVERVLSPFQERPALSGWLTTSGKVQAEFE
                     RGVLLPAALTSRQPIQILVEEGRMHHIPVLSTLLSVLNLPAVLQGQVNLEKEGMPFDR
                     LKLAGFINNGVVHTKEFLLDSPVLKISGTGRYDLLADEFDMVLATSPLGSYSAILKRI
                     PLFGHLLSGDRQGFDTAVFELKGSANKPQLRYLPAESLMTGVKGTAQLAFDILVNAIT
                     LPQKAFSMVEEGVTGGEEEDF"
     gene            complement(43584..43955)
                     /locus_tag="PK589_07900"
     CDS             complement(43584..43955)
                     /locus_tag="PK589_07900"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNJ19564.1"
                     /translation="MPRSGTIFMETRYLRQPMRLVHRSFLFLPLLLCLSAGCLWTGTS
                     PLPDGPKDAVISTNVEAKFAEDQQGGLSGIIVTTEEGIVTLTGTVHKAERKARAAELA
                     RQVKGVRRVKNDLDIRADFAP"
     gene            44057..45337
                     /locus_tag="PK589_07905"
     CDS             44057..45337
                     /locus_tag="PK589_07905"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNJ19565.1"
                     /translation="MAEPVADICVIGAGAAGLAAGIFAAERQPALRVEVLDGAASVGA
                     KILVSGGGRCNVTHDVVTPEDFFGTRHLVRNVLAAFPVKDTVAWFASLGVELKREETG
                     KLFPVTDKARTVLDALLRRSRELGVVLRPGHRVVNIERRTESEEGGTEARPGFVIRHQ
                     HGETPARRIILATGGRSLPRTGSDGGGYELVRHLGHHVTPTVPALVALMLDEDCFHAE
                     LSGLSQEVEIQTLVEGRSVDRRRGSLLWTHFGISGPVVMDASRFWCLARSRGEAAEVM
                     GNFLPGRNAEQAREWFLAQAAQNPRRSLLKVLAESLPERYAEVLCRHAGSDPQQACAQ
                     VPRAIRDRLLEALTRFRFPVVRDRGWNFAEVTAGGIPLEEVNFRTMESKLVPGLYLVG
                     EVLDCDGRIGGFNFQWAWATGRVAGRAAAASLGS"
     gene            complement(45362..46387)
                     /locus_tag="PK589_07910"
     CDS             complement(45362..46387)
                     /locus_tag="PK589_07910"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNJ19566.1"
                     /translation="MHHRHVFSLLLWQRALLLFSLWLATPAAADEIYLLNGDRLTGTI
                     VKMEENLLTIQTDYGGEIKVDWKKVQRLTATSPIKILVPGESRDVLRDFVYGTQGQRE
                     VSEAGQDTSTPLSDITAINLEPFRVAGTVSVGGNNTSGNSSTKAFNSAARLTLYAHRQ
                     RLLVEGKYNYGQANDQVTARNSLASLKHNYFISKQIFIETFGMLEKDTLQALQLRSTI
                     GSGLGYQFYETPTTTLSLSAGIAHVNEHFTNSPNTQTPSGRWSLRWEHSLWPDRVKVF
                     HRHEGFWDINAGNAFRFNADQGLRITVYKNLFFNVEYDLRLNTQPAPGRKKTDEAMIF
                     GVGYEFR"
     gene            complement(46536..47729)
                     /locus_tag="PK589_07915"
     CDS             complement(46536..47729)
                     /locus_tag="PK589_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19567.1"
                     /translation="MTIPSLLLRGLIALTVFMNLAGCLSPPTLTRAVVSYDNAITDSI
                     SKQLLLNIARAHHHQPVHFTGVSNVAATFDFRINAGATPTLTGEAGKTLLPVFGGSVA
                     ENPTISIAPIDGEEFTRRLLTPFQETKFTLLLRQGGDIDLLLRLMAKELRVDDQGEDI
                     AYRNSPTDKAGYEMFRKVVLHLSAIQDANRLYAESMLFERTWTIPAESVTAEGFKALE
                     QDYLVAYDPQQKTYRLRKPVSGRILITNYDPNTLPQEERVRLHEEAERSPMNDVSFDI
                     RPGHYGGEWPLKGEFRLRSFNTMLNFLAQSVEEEPEYAVEKDNRTPPFMDNPVKTMDL
                     LVQESSPSESDLTVQSHGKYYSVNITGPLARWNREAFKLLYQLFQMTVTEVSRSGVPS
                     ITIAK"
     gene            47910..48680
                     /locus_tag="PK589_07920"
     CDS             47910..48680
                     /locus_tag="PK589_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19568.1"
                     /translation="MRHSVWVGVMTLLCFTGCGSSAKLISGLSIEELKPVPGILSSAR
                     LLKGTIRSITGDRVEVDTGERAPRYLSLEAGGDEARGLQVGGAVEIMVNDHDDIVAYQ
                     RPAETPPTKVFRGSLGKVFNPQKERVTIRLDTGRDVGFYARSVAMEKLAALEVGETAD
                     FAVDRAYLLVDVLSLGKPRDLGVGSSLPAPQRRVEGAFVTIGDGRMRLRMKIDDIQSF
                     PVRPLLQPAIEQLSPNETVSIYLDGQGQVIDMVRTARR"
     gene            complement(48687..49571)
                     /locus_tag="PK589_07925"
     CDS             complement(48687..49571)
                     /locus_tag="PK589_07925"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ19569.1"
                     /translation="MLNWFSTSSPSDSVVITGASSGIGAACALALDTLGYRVFACVRH
                     PADGERLQRQAGPRLMPIRLDVTDSASIAAASHTVAAMVGDRGLAGLVNNAGIGVAGP
                     IELLPLADWRRQFEVNVFGLIAVTQTFLPLIRTGHGRIINMGSIAGRASMPFMAPYAA
                     SKHALEAITDALRLELQPWGIRVALITPGAIATPIWGKTRKEVDTWDATWSPDLKDMY
                     QEGFTRIKEAATAAGEQAQPAKVVAEAVAHALRSRWPHTRYLVGSDATIRSWLALLLP
                     DRLNDWIITRIVKLPRRR"
     gene            49714..51114
                     /locus_tag="PK589_07930"
     CDS             49714..51114
                     /locus_tag="PK589_07930"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HNJ19570.1"
                     /translation="MHTEKCKWALLLMMGLSLAAYVSPLNAGTDQKGVVATEKLVTVQ
                     PREIDDVLDNPGMGFADFHFGFGHPPKPDEYPHTTVAYFRWPWAELEPAEGQYNFALV
                     DGVIAQAKAKGETLAIRIVSEYKTGTPQWLLDKGVASIKETDGIFPDYNNPIFLDYHE
                     RLIRAFGERYGRSLDIDHVDIGSVGCWGEWNTACCVGAETQCKAYYPTEANQLAITDW
                     YLKYFSGTPLVMLHGGQLQYAASRGAGWRADCFGDYGYFSPDWNHMEHAYAPVLEAQV
                     VADAWKQGPVQMEVCGYIHEWYERGFDLDRILEKGLEWHVSVLNAKSKPVPAAWRPRF
                     NEFLKKIGYRFVLRELTHPASIGAGESLIIHSRWDNKGVAPIYHAWPLAYRLRSGTDE
                     VVAQWTSAADLKRWLPGPSHLAEDAVVVPSTVPAGVYTLDVGILSEDAKSATVELAIE
                     GKRTDRWYAVSRVEIR"
     gene            complement(51194..>51757)
                     /locus_tag="PK589_07935"
     CDS             complement(51194..>51757)
                     /locus_tag="PK589_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ19571.1"
                     /translation="DAAVRDHISYLDIRQDLRRTTTTMRLLESGTFHFRTPEEARNLA
                     MLLAHAYPDADRVVTGILELTLNAIEHGNLSIGYKEKSRLIEEDKLEEEIAHRLSSPP
                     YSSRVATAKFSRLPQGLSLHITDQGNGFDWQRYMDFDPERAFDTHGRGIAMANKVSFD
                     QIEYRGNGNQVMTVLRLEQAAAPALAV"
     assembly_gap    51758..51857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(52172..52849)
                     /locus_tag="PK589_07940"
     CDS             complement(52172..52849)
                     /locus_tag="PK589_07940"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNJ19572.1"
                     /translation="MRHVTGAVSAVVLLISSLSWAAASEPANDEQKTLYALGVAISQS
                     LTPFTLNEAELEFVKSGIADGVLKRSQKVDMNVYGPKIQQMQQVRSNALADVEKKAGA
                     AFLAKAAAEPGAKKTESGAIVTTIKEGKGATPKATDTVKVHYHGTLTDGTVFDSSVKR
                     GEPATFPLNQVIKCWTEAVQLIKVGGKSKLVCPSGIAYGDRGSPPVIKPGATLVFEVE
                     LLDIVKQ"
BASE COUNT        10507 a        14506 c        16619 g        11110 t
ORIGIN      
        1 gcaattcagg gttcaacgat cgtgcggagg catgccggac ggatggatcg aagtgttgag
       61 agagggagca gccgccccgt ccttcttgct cgcagaccgc gcacgatcag aatgtgctcg
      121 gcccatgcgc gcagtgaagg ccgatgcggc tactcccaag gggaatgcag ggggtaagtg
      181 gagggagcat tagatcttga cgcgcggctt cagaaagccc tcgttggacg acgcggagtt
      241 gagggccacc tcagccgacc gccaagcgcc taatgggggc cgttccggcg ctacgatttc
      301 ttgagaaatc atttcgtcgc atatatataa tcatccctgt ctatgcctaa tggacctgcc
      361 gctgtccccc agcctccgcc agatgaaaca cacccgcttt tgccatcaac ccctacatgg
      421 actacacagc gacgaagact ccgtcattgg ctgagggaaa aagcaccatc tttggccgaa
      481 ctctatgaaa ccgctgttac tcttctcgag gagcaacccc ttccaggacg aagccgtatc
      541 atcgcgcatt gcgtccgaga gatcgccaat gccttacccg ctcttctcgc cggggtagag
      601 cgcacccgtc tcgagtatga caaacgcctg gacaagattg ctctagcacg gcacagattg
      661 accaggcttc cgattcaggc tccgaggagt atggggggca catcgatttc tgatccccag
      721 gacgctctga ctcaagagtt cttcaatcaa attgaagaac tcatcaagga gcataaggca
      781 acaaggctga agcgcgatac gatagcagag aggcttttcc aagcaaatcg cccagagaat
      841 cgtcatctcg gagatgcgct acgtccagtt ttgaggcggt ggaccgacct taaggattgg
      901 tttgtcggtc acgcgcacga taatggcaag acagacgctc aatgtgattg gagtgatatc
      961 aagcgaagat ttgttctttt tgagagaaca gtactgacac ttggtcaggg gttcttcgca
     1021 acgatcgctg acctggatga gctgatccgg acgtcgaccc ctgctgacgt ccatcttgta
     1081 gtaactcaac tcggacatat tgagcattac aaatacttct ttgagcactt ggatgatccg
     1141 gcatggattc ccgcactacg agccgagggc atctttaacg cacctccgtc tgtggagccc
     1201 aatgcgatac ctcgttgggc ggcttcagag ttcctagtgc gtgttataga cagaggggat
     1261 cctaccgagg ggatgaaggc tttgagccaa attgcggatg cgcttactca acagtctttg
     1321 gtgaatccgc tggtgattcg ggatgtagtg gatgtactgc ggaaggttcc cgcggaacat
     1381 tcggtaagtt tcatgccaag actgaaacaa tgggctcacc acgacagagg ctttttgttc
     1441 tggcgacagc taggcaaact cgtaactcac cttgcagaag gaggaaagcc gcacgaagca
     1501 ctgacgcttc tgacaccgct gctcgctatc gaaggggtgc gggcagatga tgattttgaa
     1561 tattcataca agccggtcaa gccgaggatc agcatgtatg aatatattca gatcgtggat
     1621 gagcatcttc gcccagtatt agaaaggagg ggggaggagc tgtttgacgt tttatgcaat
     1681 atcctgcaga aagcagcgaa actgtctcgc cgttcacatt cgtcggaaga atgggacgat
     1741 aactcccgtg tctggcagcc agatctaagt attcaggatg aagacgatcg cgatgatgtc
     1801 agaagcgtgt tggtagtcag aattagagac gcggccgaaa cgctactcaa tgatggacgc
     1861 gtagatctcg gtcctttggt cgaagatctt gaggctcgtc gttggctgat cttccgacga
     1921 ttggccttat actgtcttgt caccacagct tcagcaccaa gagacttgat cgccccgagg
     1981 ctaacacatg aagcttcgtt ccgagataga aatcttggaa aggagtatga ctccctcctt
     2041 catatcggtt tcacacgcct gactcctgat gaacaacaga caattctcag ttgggttgaa
     2101 gctggacctg agaatattga gcaactgatc aggaactatg aaacgagcac cggccggcgc
     2161 gctacccagg aagatatcga gaagcatcac cggagctggc aatggaagcg actgtggccg
     2221 tgccacacgg cactcccggc gacatggcgg gagcggtatg ccactcttgt ggaggaattc
     2281 ggtgaaattg aagccccgct accgacgacg agagaggcaa gagatgttct tgaaggtgag
     2341 cgtgctccca gacccgaaca ggacttgcga acactcacac cagccgcgtt acgggagtat
     2401 ttgctcgctt ggaccccgga gcggaccacc ttcccttatc ctacgcgctc aggattggcg
     2461 gaggttttaa cttctctcgt tacagctgac cctgttgcgt atgccaatgc aacggaagaa
     2521 tggctgcatc tagacccgac gttccttcac ggcattattc gtggcttcac gaaagcactg
     2581 caggtagatc atcccttttc ctgggcgcct gtcttatctc tgtgtcggtg gatccttgct
     2641 gaaccgcgca caattcctgg aaggagtgtt gatcgctggg aagccgattt ggattggggg
     2701 ggaacccgtt ggtggattgt tgaactcctt cgtgtcggtt ttcaggatga gacgcatggc
     2761 attcccatcg accttcgtga tgtggcttgg caactgttgg agacattaac aatggatcca
     2821 gatccagaag cggacgagga tgaagaggat tttggaaggc caagccagtc aatgaatcgc
     2881 gcaatcagtt cggtgcgcgg tcgagctatc gaagccctca tgttctatcc ggggtggata
     2941 aaacggcaga caggagacgc tggacctcca agacttccag cagaagcgcg agctgtgttg
     3001 gataggcatt tggatctgac agaagatcca tcactggcta ttcgctcgct atatggccgc
     3061 tggcttccgt ggattctggt gttcgcccgg gattggggaa ctgccagcat acctcggata
     3121 ttccccgagt acagccaaag gtactggcta gccgcatggg agggcttcat atcttttaat
     3181 aatccatacc cggaaatatt tgaaccgctt cagccggtat atgcgcaggc catcgcgcag
     3241 cttggaagtc catcggccga ggcagatgac actcgggacc aacgagatga aagactagcc
     3301 ggtcacctca tgacatacta ttggcgtgga cactacccga tcgaagacga gaccggcctt
     3361 ccttaccaat ttttcgctcg ggcgtctgat caggttcgcg ccgaggcgat tgagtttata
     3421 ggtaggagtt tggaccgtac ggatgatcca gtggagtcgg tagttttaga ccgccttcaa
     3481 cggctatggg ctcagaggtt cgatggagca aggcaagacc cagcaacgca tcaggcagaa
     3541 ctccgtgcgt ttgggtggtg ttttggatcg ggaaaatttg agccacggtg ggcagtccaa
     3601 aaccttctgg acgtcctgcg gttgacacag agtatcgatc cagatttcca ggttctggag
     3661 cgcctgcctg accatgccgg tgcttttcca ctcgaggttc tcgaatgtgc gaagcgtttg
     3721 attgaagggc aaacgtctca tgtcgagctt tccagttggg gtgaggacct tcgaaggatc
     3781 ttctcccaga ccaggaatca cccggcaccc gaggtaaggc aggcctccga tgatgtcatc
     3841 gaagttcttg gaagatttgg tcacctcggc tatgcagatc tcctttctcg ggagtcaggc
     3901 taaacgaaga gggacccttg caatcgtcct tggtgaccta tcaatttcgc aatattctaa
     3961 tgagatcaag ctgcggtaac aatgaactct aattgacagg catcctctgt agcccataaa
     4021 ttaaatatag tgggagggtt tgtgatggca cttcttctcg cgttctacct catcttttcc
     4081 gcagtcattc ctgcatcagc gttagagggg gcccaggcca ctgccgccat ggtgatcaag
     4141 gccgatgttc tttattggga agagggcgaa ctcgtcgtca aagaattctc cggtcacgaa
     4201 gaacgtctga aagtgacgcc tgagacgaaa attgagggcg tggtggccgg acgattaaaa
     4261 accggcgaca agatcgtggc ccagatcacc gacgatcgca aggcgctcag catcaaggtg
     4321 caaattcctg aatgacggtc gagccgttcc cttcaacaga ccgtccagcg tggccgcggc
     4381 gagaaaagtg gccgatcgtg tatataccgc atggtgagcc tctcagcgaa gccggaacgc
     4441 tgccgctggg ctgtatcaac agccgttaat ccaggatcga gaacgcgccg atgaagtatg
     4501 gccctttcgc ggcctgcgcc cccctgatcc gcacttcgac cacatcctgc acccatccgc
     4561 cgacgagcca gggattgggt gccaggttcg accagtacca ccagctgccg tttgatttct
     4621 tgaaccagaa ctgaatcggc tcggtgccgg tcactcggat gattggcatg tcgaagacgc
     4681 gcggctggtg ggtcacccta tcttccatcc agattttgat ccagtgatct ttgccgtgcg
     4741 tgccgagtgt tcgcggttca tggatccaat cggcggccac gcaattgtca ccgactgtcc
     4801 gtccaaatcc cgcgccgggg ccatccccag gatcgacgaa acaaggcgtg agcaggctcg
     4861 ggctatcgaa ggtcagatgg atatgcccat aggtcatatc ctcgacgttg atgtaattgc
     4921 cgaagcagcg atgacgatag gtccagcagg cctgatgggt cgcattgcag ggctgcaatt
     4981 cgcctgtatt gtcgatgccg gtgggatagc cgaagttgcc cctcgcttgt ccgtagatat
     5041 aggtcaaggc gaatgtattg tcgaaatcga cgacgcaatc tccgagccag ttgtgagctg
     5101 cagccggctc ggcagtgagg cactgcacgc ccatgatgac tgccaggctg agcatcagca
     5161 gcgtgatgat tcgtagcggc ttcatggtga cgtcctttct tggctgggtc ttagtcgttg
     5221 aatgggcgta tccgctccct cacggtgtcg tcctgccgtg attccccagg tctcgtagcc
     5281 gctgaaaggc ttgattacgc tcggcttccg gcagaagaag ggctgcatgg tggtactgat
     5341 ccaccaaatg ctgcttctgc cggaaggtgg tggcttgaga aatctgcgtg agtagtccgt
     5401 tgagatcgac tcgctcggaa ggcatcgatt ggtgccgtga agattccgcc agcatggcgc
     5461 gcacttcgtc atcgctggct tgtcgcggac cgggcactgt ttgtccggct tccccgaccg
     5521 ggcctccagg cacgaattga tgaggcggtg gagggacaca gccggacagg atcgtcgcgg
     5581 gtatgacgat cacatatggg atggcgggtg cgaggagtga tcgatatgac acgctactca
     5641 tgacggcctc ctcttaactg ccttgcgtat gtcccgcaag ctgcctggct caaagtcggt
     5701 atttttcggg cacggcaacc aggcatgttt aagatgctct ttctgaaaac aggaaaggca
     5761 acctttgggc cacgacgtca gagggcaccc ttcgtacgta ataacaagtg ctttgagctg
     5821 gggcagctcg aggagtcgcg ctgcggcgtg caggcgcggg tatcacatac gatacaacgt
     5881 cgtatcccat ccgatacgac tacgtagctt gcaacggatg gtcgctgcct atcagagtga
     5941 aatgaggttg atggagtgtg gcgtgctgcg tggggagtct gactgtcctg agtcaaaccg
     6001 gcaggatcga ggccgatggt tctttactac gatcgcttgc gtaatcgcac cggatgacgc
     6061 cggtgtaacc agtctcgaat gagcgtgccg atgaccagaa cgggaactgc cagcaaccat
     6121 acataccaac tggcccagag cgcggcgtca tgcatgatgt atcctccagt ctatgtgccg
     6181 atggagagag caagcaccat accgtagacc gtctttctga ggcgtcgtgc cgtcgtcctg
     6241 caaatctata ggaaatctca cgagatagat gagtaacgcg gtgtcgcgct tcgttacacg
     6301 atgcggccgg caatttttgc cgtcgcaaaa agctcacaag gcgtgatgcc gttcccacag
     6361 ctgtcggcat ggctgagagt cttattccaa caagcggatc agctgcggga gccatggaag
     6421 cgtcgcgatc cccacgcaaa tcgagagtgc cacgatggag gtgaccaggt cttcgtccag
     6481 ccctgtatct gccgcaaaga tcacggcggt gacggcggag ggcatcgcgg caatcagcac
     6541 gacgatggct cgttcgatcc cggtgaggtt caggacaaag gcgacaccca atccgagcag
     6601 cagcccgccc gccatgcgca tgacgacgcc gagcgtggcc aaggcaaagg tgcggtgcgt
     6661 gacggcgacg ctgatcgaga gtcccagcac gaggctggcc aaaggcgtcg tcgtgagatg
     6721 aagaggggtg aggatctggc ggagccaatc gtgaagatgc agcccgaaga gtgatagcag
     6781 gaggctcccg cagagggccc acaatggcgg tgatgacagg aatcggacga ttgccgagcg
     6841 agccgtggtg ctcttggtgc cgtgtccgac ggcgagggcg taaatcagcg tgagggtcag
     6901 ggtagtctgg ccaaggtcaa acagaatggc gcgcgccaat ccttggtcgc cgaatgtggc
     6961 aaggacgacg gggtaggcga agtagacgga attgatgcag ccggtgccga gtaataaacc
     7021 tccctgcgtc ggacgtgtca gatgcagcct gcgggcaagt tgccaggcac agagcagcat
     7081 ggagacggtg atcaggcagg ccgcgagggg caatctccag gccgtcgcgg taaacgtgac
     7141 atgatccagg gagacaagaa ttgtagcggg cagactgatt gagaagacga aggaggcgag
     7201 ccgttccgca tgtatttttc cgagcagtcc ggatacacgg agcaacgcgc ctagaacaaa
     7261 aatggcgatg aaggcttgga gcgtggcggg catcggcggc agcctagcag aaaatcgttg
     7321 gcaagaggag gcatggctcg gcatgaagca tcccgccttt ttctgtgcgg cgaaggcgcg
     7381 agcctggtac gatggcgcac ttcatgaacc aggcgcagcc atcagtgcca ccgcggcaag
     7441 cggcggtctt ttttatcctc gtcaccgtcg tgctcgacat gttgtcgttc gggatcatca
     7501 ttcccgtgct gccgaagctg gtcgaagagt ttctgggcgg cgacacggcg caggcggcgg
     7561 aaatttatgg tctcatgggc acgtcctggg cgctgatgca gtttgtctgt tcaccgattc
     7621 agggcgcgtt atcggatcgg ttcgggcgtc ggcctgtcgt cctcctgtcc aatcttggcc
     7681 tcggccttga tttcatcctc atggcaatgg cgcccagcct ggcatggttg ttcggcggtc
     7741 gagtcatctc cggcatcgcg tcatcgagtt tcagtactgc cggcgcgtat atcgccgatg
     7801 tgaccgcgcc ggagaagcgg gccgctgcgt tcggcctgat gggcgcatcc ttcgggttgg
     7861 gcttcgtgct ggggccggcc gtcggcggtc tgctgggtgc cctcgaccca cgctggccct
     7921 tctggggagc tgcggcaacg agtctgctca atgcctgtta cggattcttc gtcttgccgg
     7981 agtcgcttcc cgtggagaag cgggcgccgt ttcgttggca gcgggcgaat ccggccggcg
     8041 cgttgatcct cttgcgctcc catcacgaac tgttcggtct ggcaacggcg aactttctca
     8101 tgaatctggc gcatggcgta ctgcccagcg tggccgtgtt gtatttgggg taccggtacg
     8161 gctggggtcc gtcggcggtc ggcttcacgc tggcggcagt cgggatatgc gccatgatcg
     8221 tccaaggcac cttggtgcgc cccatcacgg cgaagctggg agaacgccgc acattgctca
     8281 cggggctcct gtgcggcgcg accggctttg ccatttacgg ccttgcgccc acgccgttga
     8341 tctattgcct gggtattccg gtgatggcgt tttggggact tgccggccct tccgcgcagg
     8401 tgttcatgac gcgccgggtg agcgcgtccg agcaggggca actgcagggg gcgattgcca
     8461 gcctcaccgg cattgccgga ttgatcggcc cgacattgtt cacgcagacg tttgctctgt
     8521 tcatcggccc acaggccgac tggcatatgc ccggtgcgcc gtatcttctg tcgacgatgc
     8581 tgctgctggt gagtgcggct actgcgtggc gcgcgaccag gccggtgtaa gcgatttggg
     8641 tattgctgac agcgatcagg tttggacagg tggtcgagca ggccttcgtc tactgcacgc
     8701 cgtgcacgaa gagttcccgg ctgcgataga gagccagtgc ccgcatctct gctgtatctg
     8761 cttccgcttg ctggcctaat tgccggtgaa ggtctgcgag gagcgaccag gcttccgctt
     8821 cggcggcggt atcgtgtatc gcctgagcga tttgaatggc gcgttttccc agcgccacgc
     8881 ggtcattcag cgtgtctttc ccttgatcga gccgggcgag ccgcaggtgc agcatgcctt
     8941 cgaacggcag atggtgttcc gcctgggcca gcgccagtgc atccaggtag tgtgtgcgtg
     9001 cctcttccag tcgtcccagc agccaatgcg cgtccccgag cgcgacggtc gcggtgaact
     9061 gctgatcgac atggcgccga gtgtgcgcct cctccgccgc ctgctgcaag gacgagagcc
     9121 cggcatcggc cgagccttcg acgacttgaa gcaggcccaa ctgcaccatc gtctcgttcc
     9181 atcctcttgc attcctcacg cgggtaaaga gggcggcggc tttcgtcagg gcgtcgcgtg
     9241 actcatcacg ttgccccagt tgcgcagcca cgcggcccat cgccagcagg gcttcggcat
     9301 gggcggatcg atcagcagcc tgtgcaaata actgttcggc ttcccgcaag gacgcatggg
     9361 cgtcttgcag tttgccttgt cgctcatgca cgcgcgcaat cttcatgagc gtggtcgcaa
     9421 tgccctgctg ttgcttcttc tcccggaact tcgacaaggc gagttggtaa taggtcaacg
     9481 tttcgggcca atgttcctgt ttgtcgtgga cctcgccgat gcgaagcaat tcacggcctg
     9541 agagttggtt cggcgaggtg gtaggcgtga cgtctcccgg cgaaacggcc caggcttggg
     9601 caggcaggca ccagagcaag aggaaccaaa acccgatcat gctgtctctc catcaagctg
     9661 cagagtgggg gcctacgagg aagtgatctg tcgcgtactt tgcattgacg tcccggtaag
     9721 gtcaagccag taagctgagg tcgtgttcct tttctcatgt ttccgcgcat cgaccctatg
     9781 ctttcgacga tgaagaaatt cagcgacaaa tccaggaacc ggttatactc agcgcctgtt
     9841 tgtctgacgg aagcgtggca agggctcact tggaggcgga aaggaatgaa aaccatggca
     9901 ggtgcaacgg gaacccggca aaagaaacag gctccgcaac gaaaaaaggc cgggcctgtg
     9961 aagaagacca ccagggcgcg tgccggccaa ccatccggtg cggtgatcgt cctgtccgga
    10021 gccactccgc ttcgtcggca aaaggcggct gaggttctgg cggaagagtt gcaattgcat
    10081 ctggccgcgg tcgatttgtc ggccgtggtg agcaagtatg cccgcgagac cgaaaaaaac
    10141 atgacacgcg tgttcgatat ggccaatcgc cacggatcga ttctcttctt tgacgaggcc
    10201 gatgccttgt tcggaaaacg gacgcaggtc aatgactccc acgatcggta tgcgaacagt
    10261 gaagtgtcgt atctcctgca gtgcatcgag acacaccagg gactcgtcat tctggcgacc
    10321 aacgggaaga gccgtatcga ggacgccttc tctccggaaa agccggtcat tgtgttgggc
    10381 gggaccgcga agaagcaggc tggcgtcaag ccgaagccga aagccaagtc aacgccagcg
    10441 cctaccaaga aggtgagaaa gaagaaataa gccagaccgg tgaggggggt ggcgcgccca
    10501 ttccaactcg gccaaccaga attgtttcct gagaactgtt gaaagcttga agaagggggc
    10561 gctcgggcgc atggaccttc cgatacgatc ccctcgtcaa aagctcggcg agtatgtgat
    10621 cctgcccagg cttatcgata aggttcggct tcatgccgca ggtgctttgc cgttggacta
    10681 cgtgcggaat ttgttaaagc caggacttac tcttgatggc agattcttga aattcaccgg
    10741 acttgccgcg gacgcgctgg aaggtgcgat tctagcggct gaatcagact cacttgtttt
    10801 ggcatgggtg gatgaacagg cgatagttca ttcagatgca gagaaacagg cgtggtctga
    10861 gaaggcggcg gcgtttcatc cggaccctcg tgtggctaaa tatctgatga tgagccttcc
    10921 agatcttgca actagcctgg atatcacccg ttatactctc tttgatttga ttgatatgga
    10981 tgaggggcgt ttgccggttc catcgaggcc gaaggcctga accaagcaga cacgctgtac
    11041 gaggtcgctg tcgtttcagc actgcagggc cgtgatcagc gcgaccttcg ccgctcgatc
    11101 cagcgccttg atggcggcct cgcgttgcag cgcggcgcct ttgctagcca gtgattcggt
    11161 ataccgcacc gtaaacggtc ctcggtgtct cgtgtatttc gccccatttc cgcttgcatg
    11221 cgatcgcatg cgacgctcca aatcgtttgt gatgcctgta tacaggctgc cgtcagcaca
    11281 ttccagaata tagacgatcc acgtcatgag cagcgaggcc tggtatatgg atgcgcttcc
    11341 gacgcacagg tgtgtgccgg actcacgcga tcatagccga aacgtctcta agaggcgact
    11401 atttggggaa ggtggggaag accgtcagtc cgaggcttgc gaggatggcg gcgagaatga
    11461 gcgctgcagc cagtgcaacg atcagatact tccgatcatg gcgcaccgaa tcgtgaaagc
    11521 ggtacatgct ggatttgcgc aaacgctgag tcatcatgct gtgttcagcc ctgacagagg
    11581 tctttgtggc acgtgctgcc gtccgctcgt cagtgcgcag cgagtatgcc tgcccggtct
    11641 tctgagggga ataggggttt ccctgaaaga ggggggaatt atcactgtga cgagcagatg
    11701 ttccgttctg aacgccaggc tccgccttcg catcagccga tgggtggaga tgtgtgtggc
    11761 atcatcagat ggcctcattg acagtttcaa cttcagccac gtagcgtaac ggcccatgtc
    11821 gctgccatct cgcattgcgc tggaactctt tactgcgaag caggcctgga gtgcgggcaa
    11881 tgaagggaag gcgcgagtct gtgcacggcg ggcggtcgct gccgccgccg aatcctggtt
    11941 cgctcatcga ggtgaacagg ttgcacaggc ggatgcgatg acctatttgc gtcgcattca
    12001 gcagcaaggc acctttcctc ttcctgtccg ccaggcggcg gaacgcctca cgactgcggt
    12061 gaccaggcgc cacgaggcac cgtttaccac cgatccgttc accgacgctt ctctgatcat
    12121 ttcgcatctt gcagccgatt catcgagtga agggcatgct gatgtttctc cagagcgaga
    12181 ggacgatctg cagagccggc ctggagcagg tcgcattctc attatcctga tcatgggcgt
    12241 ctccggggca gggaaaacga cggtcggccg ccgcctggca gaagtcttag gctggcggtt
    12301 cctggagggc gacgatctcc atcccatagc caacatcgag aagatgcgga gtggacatcc
    12361 cttaaccgat gccgatcgac gcccttggct ggaacggctg catacggcca ttgccgatca
    12421 ggttcggatg aaccagccgg ctgtgatggc ctgttctgtg ttgaaggcga ggtatcggac
    12481 tgtagtcgag gaggggttcg gccacgccct gcgactggtc tatttgaaag gcacggccga
    12541 cgtatttcgt gagcggctcg ccggtcgccg gaaccatttc atgcgaccgg aactgcttga
    12601 tagccagctg gcgattctgg aggagccggc cgatgcgctg gtcgtcgatg ccgctctgcc
    12661 tccagatgaa atcattctcc ggatcagaca aggcttggcg gtctaacgag gcgagcaccg
    12721 tcatctggtt ccctcccggc ctctattctt tgacgtaatg gacccgcacg gtttggatca
    12781 atccctcggc gttcacggtc atcatttcac ctgccagcag tcccttgacg ctacggtaat
    12841 agagcaacag gctgtcgatg cctgtcagaa cctgtaacaa gtcgaaatgc aggtcgggat
    12901 aggtgtgaag tcccttttgg aaataggcgc tcaaccggtc gcgtccgtga agtgtgccgg
    12961 acgggtcccc ggatagcgcc ggcacaaacg gactggtaaa ctccacatct gatgcataat
    13021 gatggagaat agcctcgagg tcgtgccggt tccaggcgtc gatccacgct tcggccaggc
    13081 ttcgtgcaaa ctgttcgttc atcaccgtga acctttcatt gagagccttt ggtggtgact
    13141 agcctagggg aacggctacc tgatgagcaa gggccgatcc tcgtcaggcg gatgaaagtg
    13201 gcgctatgct tgtgagcgcg ccgatatcgc agcagacgag cctggctccc attgccctct
    13261 tcaagctggt gaaagggttg ttggtcctgc tggcgggatc aggctttctc cggtggatcg
    13321 atcctgaaat cgattccggt ctctcgcccg ttctagacgc cctgcacttg aacatccaca
    13381 cacggctgct acatgcgctc gccatcacgg tggattcgtt gcctcggcac agtctgttgt
    13441 tgatgagtct cgtcagcctc ggctatgcgg ccctcttgtt tgtcgagggg ttcggtctct
    13501 ggagcgaaac ctcctgggct gcctatctga cggtcgtgtc cacgtgtgtc ctcttgcccg
    13561 gggaatttca cgccgtcgtt cgcgagtggt ccatggcggg gctggcagtg ctggcggtga
    13621 atattgccat cgtgggatac ctggtgcgca ggctggcgga cgagacattg cgttgacggg
    13681 cctggtttgt gcgtgttcca acttcgattc tcggggaatt cctaggtggc attccgttct
    13741 ggaggcgata taccaaaacc atcagacgag aggagacatg ccatggtcga agttaactcc
    13801 atgtatcaac ctcgaatgag gaacgacgat attaaggcac aaatcgataa gcttgagggg
    13861 ctggctcatg tcaccgctgt ccatcccacc tttgacacat tcgatgaaga gaccgaaacg
    13921 atcctcacca gaatattcgg cggctctcat ccctacgtcg agtcctataa atatgcgacg
    13981 ctgggggaag ccgaagcgat cgtcaatttg cctgaatcag cgcaggagcc tctgacgcgc
    14041 gatacgccga agaagggcct gcagcagcgc cgtcaggtgc tgcaaagcat cctgaccgac
    14101 ctgcaggggc ttgaagcgga agaggccgaa gcactgacgg gagaagacca cgaagatcct
    14161 cccggcccaa gctaatggtc ctataccggt tattccgggc atgggggcgg tgaccgccgg
    14221 tgagttgtgc gtaaaggtgg tcgctgcctc cgcgagccgc ttccgaacga ctgtgcccct
    14281 cccacttccc tcgtgatcgc actcacctct ggctccattc ctccttcagc tattggcttc
    14341 ctccacgaag gacagcaaga taggcaatca agtcccggat atcttcgtcc cgcaggagcg
    14401 ttttcggata catggccgtg cctggatggc cgagtttgat ggatcgatag aggtcttcgt
    14461 cactttgagc ggtcaggctg gacggatgac gcaggtcggc cggagggtgg ggaagcgcct
    14521 tcagtcgtga tatcaaatca gccgacatcg gggggcgtga atccaagtgt ccctcgatgc
    14581 catggcagat ggcgcagccg ccgagttcgt ggtagagctg ccgcccgttg ttcgcatcgc
    14641 ctcttaagga aaaggaagac gcgggcacgg aagcgggagg cgatactgca ccgcggatgg
    14701 acgcgagata gggcagcaac gcgagtatct cctcatcgga gatggtgtcc ttcgagatcg
    14761 gatacatggc ggtgcgcaga tgcccgtgcc ggatgatctc gaatcgttgc ttgtcggtgg
    14821 tgagggtgag cgtcctggcc tgcctcagat cggcgggagc gggatcgagg tgtgcaatat
    14881 tgtcccggat gtgttgagtg aggtgcggcg gcagttggcc cttgtgtcca tcgacaccat
    14941 ggcaggtgga gcagaggccc ttgccattga agagtacccg gccttgctcc gcttcttgcg
    15001 gccctttgcc cggcttggtt ggagacgagg acccggccca aagcagtgga ccggcggtca
    15061 gtgagatcgc gagacagagg cccccgacac tggcaattcc aaggcggcct gatcgagccg
    15121 ttgctgtcaa gaacgccggc atggtcgatg tccggatgat gtggtcttta aatccaacaa
    15181 tctccatcat aattgaaggc gacggtgcag tgctagaacc gatggccaca ccgtgatggg
    15241 acctttgggg ggctggtcag acgcgtggcg accccgctac aatgtctctc gatgggtagt
    15301 atgattcata cggccatgaa tatggtggac caccggcact gcctcgcctt gtccggttgt
    15361 ttccggggca agctctgcga gcaattgatg aatcgaccgg ggcggcacat gccgaagggc
    15421 agtcgcgtct acgggccggg tgacccggcg cagagcgtct tctatcttcg tcggggattc
    15481 gtgaagttga cctctctcac cgaggacggg cgtgaattga tcttacgcct gcaccagacc
    15541 ggtgaagtgt tcggcgaact gtgtcattgc accggcgagc gccgcgaaca ggccattgcg
    15601 atggaagaca gtgagatcgt cgaactgagt ttcgatgaat ttattgccca tttgcaaagc
    15661 aatcggccgg cgttcctcct gtttctttcc aatgtcgccc aacagctgtc agcggcgtat
    15721 gatcagattc agactttgtc cttcaatagc acgatggagc gcctggtgcg cacgctggga
    15781 cggttagccg acgaattcgg cgaaccggac ggcgagtgga ttcgtctgac tcactatttc
    15841 cggcaggacg atctggccca gatgatcggc gcacgacggg aggtggtgtc gaccctcctg
    15901 aaccaactgc gtgagcaggg gctgatcacc tacgcgcgga aagatggggt actccttcgt
    15961 cgggccgaac tcgaccgatt cctggggtct actgcccaat catccgccag cctcgccgat
    16021 tccggcttct agcgccgcat atttctcctc cagtgttacc gcgctaactt acagggtcgc
    16081 tcccgttctc tatccttgcc cacgagtgca acagccaatg tcggctgtcc ggcgctgcct
    16141 cactattttt acaaggagag aagacatgcg taaggtgatt catgcctgtt cagtgctgat
    16201 gctcagtgtg gccaccgtgg cctgtgccca tacgacggat accccggctc ctgtggcttc
    16261 ttcagcagcg ggccaccagg ctaaggtggc ggaaacgaag gcggcgcttc gtgatctctg
    16321 gttggggcat atcctgtcta tccggaacgt ggccgtcgcg acgatggata aaaacgccgc
    16381 agcgcggagc gcggctgaag ccgatgtggt ggcgaacgcc cagcagattg cccggtcgat
    16441 cgaaccgttt tatggcaagc cggcggcgga tcaactcttt acattattgg ccgggcatta
    16501 ccaggggatt cgggaacatt tggatgccac cgtcgccggg tcgaccagcc agcaggatgc
    16561 ggccgtgaag gcgctgaccg ccaatgcggc tgaaattgcc accttcctca gcggggccaa
    16621 ccccaacctg ccgaaggaca cggtgatggg attgctcatg gcccatgccg cccaccatct
    16681 cacgcaattt cagcaactga aggacggcga gtatgcgcgt gaggcggaaa cctggaaagg
    16741 catgaagcag catatttatg tcgtggcgga tgcgctgacg caggccttgg ccgcgcagtt
    16801 tcctgccaag ttttaaggca tcctcgagaa gcgatacgac ggttcctcgt ctcattcgag
    16861 actcgtgctc tttcggaggc tggactcagg cggcccgcag cggattagtt gcgcgggccg
    16921 cctgatggat aactcgttcc acggggcccg cgtctcctct cgatctgtcg atggttccac
    16981 tctcgcgcgt cggaccggag gtcccgccgg ttcactgctt ggcgctacgt agttcccggg
    17041 gctattgcgt catctcgacg atggtctgtc gtcgcaaggt gcgacaatca ttggaaccat
    17101 tttatttctc gtcatcggcg cagcgcatat tgaaaatccg gaagccactt gcctagagtg
    17161 cgcagtcgaa acatccgtcc aatctcatca caggaggacc gtatggggag gattcgtcac
    17221 tgcgtagtgt cgggcaaggt gttgctgttg gccctggtgt tgagtgtggc tcccgccatt
    17281 tgtcaggcgg ctgaatgggt gcatgagggg atggccgatg aaggcaagat gacgttcggg
    17341 tttcgggtgg gtcccagttt catgacgcaa agttccggcc tctccacgac cggtccggcg
    17401 ctcaactttc agggtatgta tgggatcaat aagtggttcc gcttcgggat gatgctcgaa
    17461 tgggaaaacc atagcattga tgggccccgg aacggatcgc tcaatacgat taccctgttg
    17521 ccggtcaatc tcgagtatcg gccgggacat ttcggcgccc tgattccgta tgtcaccagc
    17581 ggtatcggcg tgaacatcaa caccaaagat caggcggatt cgtttgcctg gagatttggc
    17641 gcaggcttgg actatcccat gacgagcctc tttcagggcg cgcccaaagg cctgatgctg
    17701 aatatggaaa cggcctggaa gcgtaattcc gccgacgggg atgggtcctc gatgggcctg
    17761 ctcttcggtg tccgccatac gttttaattc tcaggtactc ctctcaacca cgtgccgggc
    17821 ggtcagtgat ggtcctttcg atcctgacgg cccggcccct tcggctgttt tcggcacatt
    17881 accgcgagaa ttcatgacgg ccgcgggtcc gcgccgagac gcgacttttt accctcctgt
    17941 ttccttcgcc cccaatgtgt tataagctga ctgtttctct agcctgatac tcctgctcta
    18001 ttgcgggtgt gtcgtcgaat taatccagac cttgccggat cgtacgatcg tgagaccgtc
    18061 aattccgaag gtgttcacaa gggggactga agcaggatga gcaagaagga aatcgatgcg
    18121 gcgcggcgac tgatgagcct gatgttcaaa gcgcatccct ggcatggggt gtccatgggg
    18181 gaccaggcgc cggatctcgt gacggcctat atcgaaatcg taccgagcga tacggtgaag
    18241 tatgaagtgg ataaggccac gggctttctc aaagtcgacc gtccccagcg gttctcaaac
    18301 ttctgccccg tgtattacgg actgattccg caaacctatt gcggtgagca agtggcgaag
    18361 ctgttcggtg cccgcgccgg ccggccggac atgatcggcg atggcgatcc tctggacatc
    18421 tgcgtactca ccgagaaggc gattccccac agtgatattt tattgaccgc ccggccgatc
    18481 ggcggattca gcatggcgga cggcggcgaa gcagacgaca agatcatcgc ggtgatgcgg
    18541 gacgatgccg tttatggaac gttgaccgat ctcaaagaat gtcccatgac cctccttgac
    18601 cggttgcagc attatttcct gacctataag caggcaccgg gctccacggc acacaacaag
    18661 gtggagatta cgagcatgta tggccgcgac gaagccatca aagtcatcca cgccagccac
    18721 gacgattaca aagccaagtt tcccgagttg gaatttatgt ggcccgccag catgaatagc
    18781 tagtcttacg aagaaaggtt tagcccctcc tgatgaaaca caccacgacg ccatcgccga
    18841 cagagccggc tgccaaggga ttttcccctg gatttgccgc gctcggatta gaagcctcgc
    18901 tcctgactac ccttgaagcg ttgggctacg aagagcccac cccgattcaa cgggaagcga
    18961 ttcccccgtt gctggaaggg cgtgatctgc ttggacaggc agccaccgga accggaaaaa
    19021 cggcggcctt tgccttgccg ctcctgcagc ggattgcgca tggtccacgg cagcggccga
    19081 cggcgctggt gttggtgccg acccgagaac tggccgtgca ggtcagcgaa gcggtgcagc
    19141 ggtacggcaa agagctgcgg atctccgtgc tggccttgta cggtggccag gcgatgggcc
    19201 cgcaactgca agcactcagg cgtggagtgg aagtcattgt ggcgacgccc ggccgggcat
    19261 tggaccatct ccgtcgcaag accttgaagc tggctgatct gcaggtcgtg gtgttggatg
    19321 aggcggacga gatgctcgac atgggtttcg ccgacgacct cgacgccatt ctcgaagaaa
    19381 cgccggcgac gaaacagacc gcgttgtttt cggcgaccat gccgccccgg attgcgtcca
    19441 tcgcccgccg gcatctgaag aatccgatcg aagtcacgat cgcgcgggag ccggtgaaag
    19501 ccggcgctgc tccgcgtgtg caacagacgg cgtatgtggt ggcccgccag cacaaggtgt
    19561 cggcgctggc ccgtgtcttg gacattgcca tgcccaagtc ggctctggtg ttttgtcgca
    19621 ccaggctgga ggtggatgaa gtgacggcgg cgttgaatag ccgggggtac cgatctgaag
    19681 cgattcatgg cgggatgagc caggttcagc gggatcgtgt catgcaggcc tttcgcacag
    19741 ggcaaaccga actgctggtg gcgaccgacg tggcggctcg cggattggat attccgtccg
    19801 tctctcacgt gatcaattac gacttgcctt catcgctgga agtgtacgtg catcggatcg
    19861 gccgcacagg ccgggccggc cgggaaggtg cggccatgac gattgtcgag ccgcgcgagc
    19921 agcggctctt gcgcgccgtc gagcagcaca ccaaggcgcg cattacgatc gcgccagtgc
    19981 cgtcgctcgg agatctgctg gccaagcggt tggagcgaac gaagtcgtcg attcaagaaa
    20041 ccctggaagc gggcggcctc gaagattttc ggcgtgtggt ggacgccttg tccgcatcgg
    20101 ccgacccggc ggatattgcg gcggccgcca tcaagttggt ctatcgatca cacgggggcg
    20161 accgggagga agaagaaatt cccgccgtcc agatgcgcgc gccggagccc tatcggtcat
    20221 cacgtccagc gtatggctcg gctccgcaag gggatcgcgg gcgagcgccc cgggggggac
    20281 cgagtcgtgg tggtcgagcg gccggcaccg tgcgcgtgta cgtcggcgcg gggcgggcgg
    20341 caggcatcag accgggcgat ctggtcggcg cgattgccaa tgaagccggt gtgccgtcga
    20401 ggatgatcgg ggcgatcgaa gtcgaagagc gattttccct ggtggatgtg caggaagaag
    20461 cggcccggca gattatcgag gccttgggcc ggacgcgcat caagggacaa aaggtcgcgg
    20521 tcagattgtt cagagactga aacactccca catgttcaaa ccggccgggg tgccccaatg
    20581 aaggccctgg ccggcttgtg ctctcgtcgc cccttccttc tcaacctttc gcccgccagg
    20641 gacttcaata ggcgtccgtg ttacctgcac atggtacgat cacgcatgcc tgcctcagaa
    20701 agctccgtga caacgagcat cccgcaacgt atggaccgcc tgccatggtc gcgctggcat
    20761 tggctggtgg tgacggccct gggcgtcacc tggattctgg acggactgga agtgtccatt
    20821 gtggccgcgc ttggcccggt cctgacgcat gcgaccacgt tgcatttgac ggcttctgaa
    20881 gtcggcctca ccgcttcggc ctatttggcc ggatcggtgg tgggcgcgat tgtcttctcc
    20941 tacctgactg atcggcaggg acggaagaag tggttcatgt tcaccctcat gctctacctg
    21001 gctgcgaccg tgatgacggc gttctcctgg aatctctgga gctttatgtt ctttcgtttc
    21061 ttgacgggtg ccgggatcgg cggggaatat gcggcgatca attccgccat cgatgaactc
    21121 attccggcgc gcagtcgcgg acatacggat ctgtccatca atggcagctg gtggctgggc
    21181 agcgcggccg gcgcgctgtt gaccattgtg ctcctgaacc ccctgctgat tccggaatat
    21241 ctgggctggc gtctctgttt cgccttagga gccgtactgg gggtgagcat cctgctcgtg
    21301 cgaagggtcg tgccggagag tccgcgatgg ctcatgactc acggcagcgt gcaggaagcg
    21361 gagcggatcg tgtccggcat tgaagcgcag atcatgcgcg atgaacaact gagggcgctt
    21421 ccggcggccg aaggctcgat taccattcac ggacgcacac ctgccaccct ggctaccgtg
    21481 gcccgtgaac tgttcacggc ctatccccgt cgcacctcgt tgggaattgt gctcatggtg
    21541 acgcaatcgt tcatgtacaa tgcgatttca ttcacctatc cgtttgtgct gacaaaattt
    21601 tacgccgtgc ccagccacag cattggtctg tatatcgtgc cgtttgcgat cggcaatttt
    21661 ctgggcccct tgttgctggg ccgctttttc gataccatcg gccgcaagca aatgatcagc
    21721 ctcacctatg cagttgccgg ttgtttgctg gcgtccaccg gctatctgtt ttttcaggga
    21781 gcctttacct ctgtcacgca aactgccgcg tggtcggcca ttttcttttt tgcttcggcc
    21841 ggggcgagtg ccgcctatct gaccgtcagc gagatctttc cgatggagat ccgcgcgatg
    21901 gccatagcct ttttcttcgt ggtagcccag ggtgcgggca tcgcggcccc gtggttgtac
    21961 ggcaagctga tcgaaacatc agccgagagt gtcatgtatg gatatctgct gggagccggg
    22021 atgatggtaa tcggtgccat cgtggagttg tggttagggg tgaaggccga agggcgatca
    22081 ctcgaacata tcgcgatgcc cctttccgcc caacctgtct cgagtcatgc gccgcacgtc
    22141 tgacagctat tcccatagga tgcacgcgta atctgcccac gggctgatgc cacggaatgt
    22201 gtcacggcgg cggcgggaca agtgcgaata ccattcatgt gtaagtatag cgatcaaggt
    22261 gaaatgaggc gcgggtgaac gttcgtccgt ggacagccgc gttgcgcgac tggcaggcgc
    22321 gagcggtggc cgatgtgttg gcgcaaaagc gcgaagatta tctcgtgacg gcgacgcccg
    22381 ctgcgggaaa aacgcgcttt gcgttgcgga tcgcccatca ttatctggcg gagcgtgccg
    22441 ccagccgtgt gctggtagtc tgtccgacga atcatctgcg aacacagtgg gcgtcggcag
    22501 cagggcaggc cggtattcaa ttggacccgg ccttgtccaa tgaacaggct tgcgaggcgc
    22561 gtgattatca tggcgcggta gtgacctatc agcaggtctg tctggcgccg gacgtgtttc
    22621 ggcgcgcctg ccgaaaccgg aagactctgg tgattctcga cgagttgcac catgccgggg
    22681 acggcaagga ttggggcaag gcgcttcgtg aatcattcgg cgaagccgtg tttcgcctgg
    22741 ccctgtcggg tacgcccttc cggtcagata acaatcccat tccgtatgtg cggtatgagc
    22801 aaggcgagag ccaggcggat tttacctatg ggtattcgca ctccatacgg gacggcgtct
    22861 gccgtccgat tctgttcccc agttacgagg gagaactgac ctggttatcc gacgggcggg
    22921 aacaccgggc cacgttcgaa gacggattga cctttgagcg ccagcgcgag cggctcaaaa
    22981 ccgccttgct ccaggaaacc tggctgggtc cggtgttgag tgatgcgcat gccgagttgc
    23041 agcgcctgcg caaacaggaa caggcggatg cgggcggtct gatcgtgacc atgaatcagg
    23101 accatgcacg gcaagtggcc gatctggtgc atcgactcac gggcacgcgt gcgctggtcg
    23161 ccgtctccga cgatccttcc gcctcgaaaa cgatcgaaac ctttgcccac cacaaaacgc
    23221 agcaatggct ggtggcggtg aacatggtga gcgaaggcgt cgatattccg cgcctccggg
    23281 tcggggtgta tgcgaccaac gtgttgacgg aaatgtattt ccgccaggtc gtcgggcgct
    23341 ttgtgcggat gcaggacaaa gtgccgaaac cgcaacgggc ctggctctac ctgcccaaag
    23401 acgccacgct ggtgcattat gccaagcgga tcaaggttga gcgcgaccat gtgctcgagg
    23461 agatcatgcc ggcggtgcag cggacgttgt tcgggacggc agcgacctcg ctgaaagaat
    23521 acattccgct caatggtgtg gcgcggctcg atgccttgat cggcgcggat gaaggcgagc
    23581 catcccctgc agggaagggc cacccggagc cggcagtggc gctgcatgac caaaagaacc
    23641 agttgcggga taaacatcgg cttctcgtgg gagccgtggc gagaaagtgt ggaatggatc
    23701 atcggcaact taacgcggaa ttaatcaagc ggaccggcgg ccgcgtggat caggcgacca
    23761 ttcctcaatt agagcggcgg attgccctac tggagaagtg gcgggattcc ggattcgaca
    23821 aaaagcgctg agcaatcaaa gtatggtaag catagcctgc gcgatgagtg agcaaagaac
    23881 ggtatgaagc aatcattgga gaggaagagg accatgacag ggaagaaggg taagcggccg
    23941 gtgagcgcgg cagcgaagat atcgagaaaa aaagttggaa tgaaggtcgt gaagaaggct
    24001 gcccccagga agcaggcacc ggcgcgccgc gcgaaggtga cccggacgaa caaatcctcg
    24061 gtggcagtcg ttgtcttgtc cggttccacg cccctgcgca ggaacaaggc agcggaatta
    24121 gtcgcggaag aattgcagct ggacttatcg aaagtgaatc tctcctctgt ggtaagccag
    24181 cgagttgggg aaacagagaa aaatatcaac cgggtcttcg acgacgctga acgcagcggg
    24241 tcgattttgt tctttgacgg agccgatgcc ttgttcggca aacatcggcc agccaaggat
    24301 ggcgcaagcc attacgccaa tgccggagca gcccatctgc tgcagcgcat cgaagaccac
    24361 aacggcctgg tgattctgac gaccaacggc aaaagccgga tcgaccaagc cctatcgaag
    24421 caggctcgcg tatcggtgat ggtgggcatc gaaaagaaga agcggaaaca aaagacggcc
    24481 tgatgagccg tccacttcaa agggcgagat cgaagtcctg aatacaggat gctgcaaaag
    24541 ggttgttcaa caaagccgca gcaagcgtaa ggctgaggag taactcagaa gtccattgag
    24601 ggcccgagtg atgcgagaac gccgctgaca gactggttcg gcatttttgc ggcttactcc
    24661 gtttcttcgc tcgcctccag gcacttcgtg cattgctcga ggtcgggcac gccatgggcg
    24721 ctgcagaagt gccgatggca gagaccgcag agcataaatg tcattttaat tcgatcttcc
    24781 gcttcgcagt agtaacagtt ttttgcgctt cttgccttgg gcatggtcct tctcctcgca
    24841 tcattgtccg gttcataggt cgtctcgtca cttgacggtg ccactcgggg tcggcggcgt
    24901 attcgaacgc acccattcca ggctcggcat gggaacggca gcgtctcaat tgatgacggg
    24961 aggcgggcaa gtcacccagg ccgagttagc ctgaggcgct aaggacagca aaaggtgtga
    25021 tcgtgctcgc agtcatccta ccaggaagcc ccgaccggct ttcctggacg gttagtttgt
    25081 tataacacat tttctcatgc gtctgcgcag cagcacgttg cgtgggagcc ccgtctcctt
    25141 gcgagaacgg ctcgtctact gtgcggttgc ctgctcgact tcttcccgca agaggaactt
    25201 gatggagggg ttggatttga tgcagtaggc cagcaggtgt tcggtggtaa acaacatgac
    25261 ggcgcggcct tgttgatcca cgaggcggac ggtatcggag ggccaataga cagcagaaat
    25321 ggttgccgca tcaccgacga tccagcgcgc cagactatgc ggcatacgct cgacggtggt
    25381 tttgactccg tattcgttct ccagtcgcga catcaccaca tcaaactgca gctcacccac
    25441 cgcagccaat accggttccc ggcgcacgtg gtcgggtgaa taaaaaatct gcatgacccc
    25501 ttcttcttcg agctgcttga gtcccttctg aaactgcttg tgcttggaga tatcctgcgt
    25561 ccgcaacagg ccgaaacatt ccggcggaaa gtgcggaatg ggatcgaagg tcagtgaagg
    25621 atcggaggtc agcgtatcgc cgatggtaaa cagtcctgga ttcaccagtc ccactacatc
    25681 gccggggtag gcttcatcga tcgtttcgcg gtcgcggccg aaaaggcgat gcgggcgcgt
    25741 catgcggatt tttcgaccca gccgggcgtg atggaccatc atgtcctttt cgaatcggcc
    25801 cgagcagatg cgcaggaagg ccatgcggtc gcgatgttgc ggatccatgt tcgcctggat
    25861 cttgaatacg aaaccggaaa aggtctcgtc cgtcggctgc accaggccct gggcgctgtg
    25921 tcgcggtccg ggaggcgggg caagtttcgt gaaggcgtcg agaaacggtc cgacgccgaa
    25981 gttggtgagc gcgctgccga agaacaccgg agtcagttcg ccggccagga agcggtctcg
    26041 atcgaacgag gtgccggcgc ccgtcaagag cccgatttcc tcctgcagcg ggccataggc
    26101 gtcgcccagc gtctccgcga gtttcggatt cgggaaggcc tcgaccgtca tcggggcgcg
    26161 acgttggttg tgcgaggtgc gctggaagaa caggacctgg cggttctgaa aatcgtacac
    26221 gccttgaaaa ccggatcctt cgccgatggg ccaattgaac gggaccgccg tcatgcccag
    26281 cgtgcgctcg atctcatcga gcagatcgaa ggggtgccgg cccggctggt ccatcttgtt
    26341 gatgaaggtc aggatgggga tcccgcgttt gcgacacacg gcaaacagct tcttggtttg
    26401 cggctcgata cccttggcgc cgtccagcac catgaccgcg ctatccacgg ccatgagggt
    26461 acgataggtg tcttcgctga agtcctggtg gcccggcgtg tcgaggagat tgacccgcgc
    26521 cccttgataa tcgaattgca gcatggtcga ggtaatcgaa atgccgcgtg cctgttccaa
    26581 ttccatccag tcggatttgg cctggcgttg ggtggagcga gcctgcaccg caccggccaa
    26641 gtgaaccgcg cccgcataga gcagtaattt ttccgtcaac gtggttttcc cggcatccgg
    26701 gtgggaaata atggcgaacg tgcgccgacg cagcacttct cgccgtaact cgtcggaggc
    26761 ggacttctca accggcaggg acataacgtg ctcttggtgg ttaggggaat cgacggaaca
    26821 gggggtatgt ggccgcctgg cgtcgctggg ttgcaacgcc ttcttttatc agtctgagcg
    26881 gggcggcggc aagaaggggg agaggggggc cgggcaacct gtgtggtccc catgctcgcg
    26941 caacgcgcgg tcgcggactc ccctcgttgg gtgatgtaag tcccttgaga gattggccct
    27001 catggagaca tacacctcac ttggcatata ctcccactgc cgcataacgt tctgaatgtg
    27061 gagtctgaat atgtctagca ttccatgagg gccgcatgaa cgttgatgac attataagct
    27121 acggtgagtt ggtcagcgct gaaaaattga tgcttcagaa gggaatgaat tttggagtgg
    27181 gaaaggacta ttccattcta ctcatgtctc aacgagccaa cgctccttat cgagatgtag
    27241 ttgatgatgc cacaggaata ctgatttatg aaggccatga tcagccacgc acaaaggatt
    27301 gtcctaaccc caagacggtg gatcaaccaa tgaccacgcc aaagggaaca ttgaccgaaa
    27361 atgggcggtt ctttcgagcc gccactgatt tcaagcatgg actaagagaa aaggcggagt
    27421 tagtcaaagt ctacgagaaa atcgctgttg gggtatggtg ctacaaggga ttctttgagc
    27481 tgtttgacgc gcaccttata tcacggctag aaaaaagaag agtgttccag ttcttcctca
    27541 agcccgttca aaagaaagcc tttggtcgaa taggggaact atcacacaaa cgactcattc
    27601 ccacgcatgt gaaagtggaa gtttggaaac gagatcaggg caaatgtgtt caatgtggtt
    27661 tgcaggagaa tcttcattac gatcacgaca taccctattc gaagggtgga agcagtcttt
    27721 cagcgcaaaa cgttcggatc ctatgcgcaa aatgcaacct gcagaaatcg aacagaatca
    27781 tgggtgtttt cccctggcta tgggtggcat cgagccgtct gctggagctt ggatggcacc
    27841 catgattctg agggacgttg ccagagcaaa agttgaacta gtttagaatg cgaactaaag
    27901 cagctgcagt aacatgttga atcatccccg cgatcctttg catggcatca cgctggagaa
    27961 gatcgtcacc acgcttgtcg agcggcatgg gtgggctgaa cttggccgtc gtctgccggt
    28021 gcgctgcttc ttgaataacc cgagcgtcaa atcgagtctc acgtttctcc gcaagaccca
    28081 gtgggcccga gagcgggttg agggaatgta tattgcggaa ctggagtgag ggcgcagtta
    28141 ttcatcgaag gtgccacatc agtcgaacga cacacgactt gtcccacagg gctctgctag
    28201 gctggacgcg cgtcgtccct gtcgatgttc ctgcgacttg atcaatcccg cccacctctg
    28261 tagatatgaa gaagactcgc ttacgatcgc ggaggttcac gttcgtgcca atccccctca
    28321 cgcttgcctt ctgcttcctg ctctcgttga tgcagatctc tgctgtctca gctcagtcga
    28381 cctcctcgtc taccgataag gcggttcgtg ccacgttgga aacggtcggg gtcaagttgg
    28441 accagccggc ggccaatgga ccagctccag atgccgccgc cgcggatctc caacacaaac
    28501 gtgcagcggc agaaaaggaa ctgagcgagc tcaccacacc acaagctcag cgcaaaggaa
    28561 cggcggggaa ccaggtggat ggccgcacgg cggagcggcg ctcgctgctg cagcagttgg
    28621 tgcaaatcta tgagcaacat ctcgatgcct tgcgcaatct cgagcagatg cggcaacggg
    28681 tgcgggaagc tgaacgccag gaaaaggagt gggatggatt ttctacgccg gcgccgtatt
    28741 cggtgttgaa aatcgatgag ctgcgcgatg cggttcgatc gatgaccctc gcctcacaag
    28801 gcgcccaaac cagactcaac atgaccgaaa gcctgaccga gagcacgcag cgcagcctca
    28861 aagcctcgca ggaaaaagcc cggcaattgt cggagcgtct cgaagaggtc caggatcctg
    28921 ccaagcggga ggcgctcaca ggagaccgcg atctggctcg attgcgcgag cgagtggaag
    28981 gcgcacgagc cggcatgttg gaggcggaaa agaagcagat ccaggaagag gtggcggaag
    29041 cgcgtcatcg tatggcattg ctgacccgcc agcgcaccgt tgccgaacag gattcggcat
    29101 ttacccaaca ggattacgac aaaatcaaaa agcggctgga tgccgaacat caatcgttga
    29161 cggatgaaat cgagcgagcg gtcctcgaac aatcgacgca acgccaggcg gttgcggccg
    29221 gagaagctgc gctcgctgcg gcgaatgcca aagactcttc gtccaagagg aacgcctccc
    29281 ggccgaaggg ggatcgcctg accgcgctca ccgaatcggt cgaactgaag cgtctgcaat
    29341 tcgacaacgc caatctgcac gtggagttgc tgcgcgagat gctgacgggt cttgagcagg
    29401 aacgacatat ttgggacatc cgtttcgcga ctgcccagga aacgctctcc gtcgtcgaag
    29461 agcgggaggc gaatgcgaag atcgccgccg ccgccaaaca gattcgaggc tggaaggaat
    29521 acggcagtca gcaactgagc atggtgggca atcacatcag tgatgtggaa gaccgccttg
    29581 cggaggctcc ttcggccgcc agggcgcaag tgttcacgga caaactccgg ctctatcgcc
    29641 accgtgaaga tctgtaccgg cgcgcgctcc agcggacgga ttccttgttg gggttgatcg
    29701 acaacaaaca ggcggaattt gcccaccgtg agcaggccag gtcagtgttc gcgcgcatga
    29761 aggagtgggg gcgagcctcc gtggtgatgc tggggaatgc ctggcatgtg gaattgtttg
    29821 ccgccgaaga caccattgaa gtggacggca agaacatcac cggtcgacgg agcgtcacgg
    29881 tcgggaaagt cgtgacggcg ctggcgattt tgatcgtcgg gtactgggtg gcgggaatca
    29941 tggcccggtt tgccgagcgc caggccatcg ccaggctgca gatcgatccc aacgtcgcca
    30001 acatcattcg gcagtgggcc ctggcctgct ttttcctcct ccttgtgatc gtcaccctca
    30061 tgtcagtgaa aattcccatc accgctttcg cgtttctcgg cggcgccctg gccatcgggg
    30121 tgggattcgg tacgcaaaat ttgctgaaga acgtcatcag cggactgctg ctgctcatgg
    30181 aacggccgct gcgcgtcggc gatgtcatcg aggtggatgg aatcagaggc atggtcacca
    30241 ccatcggtct gcgctcctcg acaatccggg acatcaacgg cgtggaaacc ttgattccca
    30301 acagcaatct gctcgaaaaa aatctgacga actggaccta ttccagcttt cgtaaacggt
    30361 attcgctccg catcgcggtg gcgaccgggt cggatgcgcg gcatgtgaag gagaagctgc
    30421 gtgagctggc cggtgagcac gggcagattc tcaaggagcc ggagccgtat gtattgtttg
    30481 aagatttcag cgagcatgcg ttagtgtttg tcctccacta ctggatcgag atcagtcccg
    30541 gtatcgacgc ggccacggtt gccagcgatc tgcgcttcat gattgaacgg acttttgctg
    30601 aagagaagat cgcgaggaaa taacggccgc ctgcctcatc gggctcctcc ctctgttttc
    30661 aaggaatcgc atggccccca acgcgacgat ttgtaaggcg gtcctccata ttgccgacgt
    30721 ggatcgacag tattacgaag accacagcat caccttggcg cggcatccat cggaaaccga
    30781 cgagcgcatg atggttcgcc tgcttgcgtt tgcccgtcac gcgcatgaag ccctgtcgtt
    30841 tggccgtgga ttgagcaccg aggatgagcc ggcgttgtgg cagaaggatg cgaccggtct
    30901 gatcgagtta tggatcgaag tgggtgtgcc ggacgaaaag acgattcgcc gggcctgcgg
    30961 tcgcgccagg caggtgtggg tctataccta tggtgggcgt gcggcggacc aatggtggaa
    31021 gcagcacggt gccgcgcttg accggttgaa caatctcgcc atcgtgaatc tgtctttgga
    31081 agggagtcgt gcgctgacga gtctggcgca gcggagtatg gatctgcatt gcaccattca
    31141 agatgggcag gtgttgattg gagacgggac gaatgcggcg cagatggacc tgttgctgtg
    31201 gaaagagccg gcgcgtgaat agtcaacgag gaagaggttc ggcatgaaag aaaagacgcg
    31261 cattcctacc gatggagaac ccgtgacgtg gaagagcccg tttgcagcgt tgaaagacgt
    31321 ggtcttgccc ccggccccgc cgcgagagca ggccaagcag ctcgggacac cggcaccggc
    31381 tccggcgaag aatcgcggcc gggtggatat catcaggcag acggcgcatc gcggcggcaa
    31441 gacggtcacg gtggtcacgg gatttgtcgg catcagtcag acggagaaag aacagctggc
    31501 gaaggagatg cagaaggcct gtggcgttgg agggacggtg aaggaaggtc gcatcgagat
    31561 tcaaggagac cggcgtgacg acatcgctcg tattttgacc aaagcggggt tccggccggt
    31621 ctttgccggt ggctgaacag tcatggcgga accgctgaag aatagtttcg gggccgatgt
    31681 tccccggacc attgcgcgaa tgattgccgc cgtatcgcct cgattcgacg agaaggcatt
    31741 cgtccggtcg gtgctggatg gttatgacga gttggaattg atgccgcgcg cctggaagat
    31801 cgcgcatgtc ttgcgacgcc atttgccgga tgactatgaa cgggctcttg agatgcttct
    31861 ggcgtcgctt gatcagcagt cgaagcgaaa gactgcctca ggcatgggcg cctttttgtt
    31921 tcttccgcat gtcttcttcg tggctgaata tggattggac catttcgaag cctccatgcg
    31981 cgcacagtat gtactgacgc aacgattcac ggccgagttc tccatccgcc ggtatctcga
    32041 gcgacaccag gctgccacgt tggcccgtct tgcggaatgg acggctgatc ccagtgaaga
    32101 cgtgcgacgc ctggtctcgg aaggcactcg cccgcgattg ccctgggcac cgagactgcg
    32161 cgccttccag gctgatccgc ggccggtgct ggccctgttg gagcggttga aagacgatcc
    32221 gtcactctac gtgcgccgca gtgtggctaa caatctgaat gacatcggga aagaccattc
    32281 cgaggtgctg gtcgagacgg ccaggcggtg gctggagaac gcaacggccg agcggcggtg
    32341 gatcattcgc catgctctcc ggtcggcggt caagcgggga gaagcaggag cgctccatgt
    32401 gctgggcttc ggccacacac cgttggtcgc cgtggagacg atccgcatcg cgccgtcacg
    32461 gcctcggatc ggatcgtcag tgggcgtcac ctgggaggtg gcgaatcaag cctccggggg
    32521 acaacgggtg ctgctggatt tgcgcgtcca ctacctcaag gcgaatggca aacacagtcc
    32581 gaaggtgttc aaattcaaaa ccgtcgaact ggcggcgcgg gagacgcttc gcttcagcaa
    32641 atcgttggcc ctcacgcaac tcaccactcg cacccactat cccggcttac accgcattga
    32701 gctgctcgtg aacggccacg cctatccgtt aggggtgttt cacatcacgg cagggtgacc
    32761 ggcgcgttgt cgcactaggt tgcggcgatc tgtctgggat ccagcgtctg tcccgtcatg
    32821 ctgccttcca cgcttgccac ataggcctgc gcgacctgat cagcagggat gccgatggaa
    32881 ggatccatac ttctggcgat taaggtttcc gtcacccaag gcgggctgac gacgttcact
    32941 cgcaggccgc gcggcagatc gatggcggcg gcgcggacaa atccttccag tccggcattg
    33001 accatactga tggaggcgct gcccttcata ggattctggc tgagaactcc gctggtaagc
    33061 gtgaaagagc cgtggttggt cacgaagggc cggccgatgc gtaccagatt cgcctggccc
    33121 atgagtttgt tcttcaggcc gatgaagtag tcgtcatagg tcagatcatc caggctcgca
    33181 aacttggcaa tcccggcggt gctgaccacg gcatcacagg tgccgatcgc cttgaacagg
    33241 cttgtgatgg aatccggcga ggccaggtcc acctgaaagg cgcctttctt gtgcccgacc
    33301 accaccacct catgccgcgt ggagagccgc ttcacgacgg ccgatccgat agtgcctgtt
    33361 cctccgatga cgatcactcg catggtaatc ctccgtcgtt catgtttcgc gcagtgtagc
    33421 gaggaggcct ggagcggcgc aagcaggcgc ccttattccg ggctgtcgat cacatactgc
    33481 ggcctggccg acggctcgac agcgggttga agatgccggc aggggggaat atcggcctgc
    33541 agccgccgta acaacaccgt gatcttcatg ttggctggaa tgtcgcagat ccacacgtgc
    33601 atgcccgatt cgagatcgtc ataatgccgc tggaccgaaa atcccggctt ttccatgaaa
    33661 taggccgaga gaaatccgtt gatggcttga acggtccagg gatggtcttt gatgtatcgg
    33721 taggtcagtc gcagttccac gacctgattc gcctgtgctg cttccatgtc tgttccgctc
    33781 ctctcgtggt gacggcgcac tctagcaggt tgtggagaac ggtcgccagc gtcgtccggt
    33841 gccttggccc aaccaggtga aagattgaga ccgccgcaag gatctgtgta aggtacggca
    33901 gggaggatct ggtcatgttg actgcctata atcaggtgcc tgttccctcg ttcatgtacg
    33961 gaacggcgtg gaaaaaagac gcaacgacac aattggtaca gcaagcggtg gcggcgggct
    34021 tcacggccat cgatacggcc aaccagctca tccactacca ggaagccctg gtcggagagg
    34081 ccttgttggc gttggcaaag cagggcacgc cgcgtgagcg gctgttcgtc cagacgaaat
    34141 ttacgccggt caacggacag gatcatcgga cgccctatga tgcgcaggcc gatctcacca
    34201 cccaggtgaa gcaatcgttc ggcagttcgc tcgcgcatct gcataccgac tacctcgact
    34261 cctacgtgct acatgggccc tacgcccgcc gggggttgag tgacgccgat tgggaagtgt
    34321 ggggagcgat cgaaagtctc tatgcggcgg gaaccacgcg aatgatcgga atcagtaacg
    34381 tgtcggcgga tcagctccgg ctgctctgtg aacgggcgac gcataaaccc atggtcgtcc
    34441 agaatcggtg ttacgccgcc ctgggctggg accgagacgt acgggagatt tgccgtgcgc
    34501 acgggatcat ctatcagggg ttctcgttgc tcacggccaa ccgggatatc ttctccgatc
    34561 ccgatgtgcg caccatggct gccaagtacg gcatgggctt ggcgcaactg gtgttccggt
    34621 ttgcaatgca gatgggcatg ctgcccttga ccggcacgac cgaccagcaa catatggccg
    34681 aggatctccg gtccgatcag ttcaccattt cgacagagga cctccagcgg cttgaaacgg
    34741 tcgggatgta gccgaatgcc ggcatatcgc cggttctgaa tccatggaca gcctgcatca
    34801 actggaaatc attatcctgc tgctcgcggt ggtgctggcc ctcaccacga tcgcgcagaa
    34861 gatcagcatc ccgtacccga tttttctggt gctgggtggg ctggtgctgg gcgtggtccc
    34921 gggcctgcca acggtgacgc tgcacccgga ccttgtgttt ctggtgtttc ttccgccgat
    34981 tctctgggcg gccgcctatt tcacttcgtt gcgggagttc cggcagaacc tccggccgat
    35041 ttccctccta gccgttggcc tggtgctcgc gacgaccgcg ggggtcgcgg tggtggcgca
    35101 tgcgttgctg cccggcatcg gatgggcgga agcgatcgcc ctcggagcca tcgtctcgcc
    35161 gccggacgcg gtgtcggcga ctgccatcgg gaaacgcctg cgcatcccgc gtcgtgtggt
    35221 caccatcctg gaaggagaaa gcctggtcaa cgacgccacc gcgcttgtgt tgtaccgtgc
    35281 tgccgtgggt gcggtggtga gcggctcatt cctgctcggg ggaacgctgc tcaattttct
    35341 ctttgccgcg ctggcggcac tggcgattgg tcttgccgcg gcctggctca cccgttgggc
    35401 gctgtgtgtc acggaggacg gatatacgca gatcggcgtg acattgctcg ttccctatat
    35461 cgcctgggtc attgccgagt cggtccatgc ctctgccgtg ttggcctgtg tggccggcgg
    35521 gatcacgctt cggcagtatt tcagcggcgc ggtggcgccg gccaccaggt tgcaggctcg
    35581 cgccgtgtgg gagctgttga tctttgttct gaatggcgtg atttttattt tgatcggatt
    35641 gcagctggga gccctccggg atgccgtccc tgcggggcag tacggccctg tcgtattcgc
    35701 ggggctcatg gtgagcgcga cggcgatcgt cgtgcggttt ctctgggtgc cgctggcggc
    35761 cctgatcccg cgctgcctga gcgcgaccct gcgcgcgcgt gacccgatgc cgtcgtggaa
    35821 ggggctgttt ctcatttcct ggaccggcat gcgaggcatc gtcacgttgg cggcggcctt
    35881 ggcacttccg atgacgacgg ggaccggcgc gccgttcccg ttccgttcag agatcatcct
    35941 gatcagcttt gcggtcatcc tcgtcacatt ggtgctgcag ggactgtcgc ttccgccgat
    36001 tatccggtgg ctgcgcctcg aagacgaaga tgatctggat cgggagcaac gcctggcgcg
    36061 cgaacatgcg gcgaccgcag ccctggcccg cctcgatgag gtcatgaccg aggactgggt
    36121 accgagggag caaatcgaac gggtgcgatt gcgctatctt cgccggctgg aacatctcaa
    36181 tcggaccggg ttgaatgagg aggagcgtcc ggagggaagt gttgaaaccg tccagcgttt
    36241 gcaccatgaa gtgctcacag ccgagcgagt cgccctgatc gggctgcggg acgacggcac
    36301 gatcagcgat gaagtgttgc atcacttgga gcaggaactg gatgtcgctg cgctgcatca
    36361 gggcgtcggt gagcgtcgcg cgggaaaggg tagaatctga catgccgatc catattgccg
    36421 cagcccagcc ggtcgatgcg ccgatcattg cgaagttggt gggagacctg ctggcggaaa
    36481 ttgtggctgc gatcaagacg gccgccttcg gattttcgtt ggctgagacc gaagcacggg
    36541 ctcgcgcctg gctggaggat ggaaattata cggtgttgct ggcgcgagat ggagaaggtg
    36601 ctgcaggatt tatcgccctg gccgaatgtc gtgccctcta tgcggaggga cggttcggca
    36661 tcatcccgga gttgtatgtc tcgccggacc ggcggtcaca aggcgtgggg ggcgcgttgg
    36721 tgaacgaggc caagcgtgtg gcccgctcga acggttgggc caggcttgag gtcaccacgc
    36781 ctccgttgcc gcagttcgac cggacgctgg cgttttatga gcggcagggg ttcagcgtgt
    36841 ccggcggacg gaaaatgaaa gtgtcggtgt gatccggcca gtggttcagg aagaacgaac
    36901 cggttgtggc atcgcggcag cggccgcgct ggcaggggtg tcctatgcgc gtgcgaaggc
    36961 cgtggcgaaa tctttaggca ttgtcgcttc ggaccgaaaa ctgtggtcag agacagaata
    37021 tgtgcgagac ctggtggcgc agttcggcct gcgagccgca cgaacacggc agccgtttca
    37081 ctcatgggcg agtttgccgg attacgcgtt gttggccatc aagtggcacc tggagcaagg
    37141 gcgaccattc tggcattggg tcgtattcgt gcgagagaac gaccggtcgt acgtggtaga
    37201 ttccagcccg acattgaagg ctccccttcg gaccgacttc ggacgtatga ggccggtgtg
    37261 gtttttgact gtgacgatga agaggtagcg agtgaatatt gccattattg gaggcggacc
    37321 ggccgggctg atggccgcag aagtggcgct ggccggaggc gcgcaggtcg cactgtacga
    37381 ctccatgcct tctgtgggcc gaaaattttt actcgcgggg aagggcgggt tgaatctgac
    37441 gcattccgat ccatccgatc tttttctcag cagatacggc gagcgccggg cacaggtcgc
    37501 tccctggttg gccgagtttg gtcctgatgc gctccggacc tgggcacggg gcctcgggat
    37561 cgagacctac gtcggctcat ccggccgcgt cttccccacg gatatgaagg ccgccccgct
    37621 gttgcgcgcc tggttgcggc gcttgcgtca ggatggcctg accatccacg tacggcaccg
    37681 ctggtgcggg tgggatgaaa agaaagccct tcgattcgag acgccccatg gtgcgacggc
    37741 cgtacgggcg gatgccgtga tcctggcgct aggtggaggc agttggccgc aactcgggtc
    37801 ggatggtgcc tgggtcccgg tgctcgccgg gcgctcgatc gcgattgctc ccttgcggcc
    37861 tgcgaactgc gggtttgacg tcggctggac ggagcacttt cgaacgaagt tcgcggggca
    37921 tccggtgaaa acggtcggcg tggtggcaaa atctccaacg ggcgcggtga tgcgtcgcat
    37981 gggagaattc gtgatcactg acaccggtgt ggaagggggc gtcatttatg ccgtggcccc
    38041 gtttctccgg gatgagatcg aggccaatgg acgaggcacg ctgcggttgg atcttgcgcc
    38101 ggaccgtgaa ctgccacgcc tcatcaaaga tctgtcgcag cctcgtggaa aaaagacgat
    38161 ggcgacgcac ctgcagcgcc gcgcgcatat cgatggagtg aaggccggat tgctgcgtga
    38221 aatcgtttcc aaagaggatt ttgccgaccc gacccgcctg gccgctgcca tcaaagcctt
    38281 gccgctcacg ttaacggcgc cgcgcccgtt gcacgaagcg atcagtacgg ccgggggagt
    38341 gacctgtgag gcgctggaca agcgattgat gctcaaagcg cttccgggcg tattctgcgc
    38401 gggagaaatg gtggattggg aagcgccgac gggcggctat ctgctcacgg cttgttttgc
    38461 cggtggacgg accgccggtg cgggggccct ggcctggctc gcggagcgcg cagagcgtgc
    38521 caaggcataa cgtgacgatc gacgggatgg ccttggtcgt gccgggtatt ggcaactcgg
    38581 gaccgcgcca ttggcagacg ctgtgggaac agcaacatcc acgttggcaa cgagtgcagc
    38641 aacgcgattg ggatcgtcct gtgctgacgg aatggctgcc ggcgctggag tcggcgcttg
    38701 ccgggatttc cgccccggct gtgctgatcg ctcatagtat gggctgcctg ctggtcgcgc
    38761 actgggcgca acaggcttca gttccagtgc gtgcggcact gttggtggcg ccgcctgatc
    38821 cgaggtctcc cgcgttccct gcgtcagcgc gaggttttga gacagttcca tctggcaggc
    38881 tgccttttcc cactctcttg gttgccagca gtaatgaccc tttcggctct gtggcctacg
    38941 ccaagcaatg cgcgaccgtt tgggggagtg ctttcgccga agccggcgca atcggtcata
    39001 tcaatgccga gagcgaaatt ggcgcatggc ctgccggtct cgtcttgctc gaacggttgc
    39061 tcgcgtcctc gtgatgtgcc atacctgcca caggtctaag gctgcaactt cctaacaagt
    39121 cttcccgatc catccatcta gatcagtatg cgctggtggc cccggtcggc cagcgtcccg
    39181 gtggtgagat tccagtcgaa gaggaagatg gcggattgtt cgcacgagag cgacaggagc
    39241 cgtttcccat tgtgagcgac cacactttcc gcttttctaa aatcgagagt gcggtcatgc
    39301 cctgtgcgat tgcagacgaa caacggtagt cccgtgtctt tcgtgcagcg ctcccactcg
    39361 tcgttcggac cgtggagtcc cggcgcccag gcagccgaag acacgaggac agtggccccc
    39421 tgcgctttca agctcttggc aatgcccggg gtataggcat cggcgcagat gaggaggccc
    39481 acgcggccga atggcgccag acgaaatgca gtcgcgtctg tgccgggggt ggaccaggcc
    39541 tcggacccga cgcgcaatgc gttgatcttt ctgtggctgc ccgcaatgcg accatcgggg
    39601 ccgatcgcaa ataccgtatt gtagagctgc ccggatcgtg gctctttctc cgggtgcgac
    39661 aggaatagcg tgactccgag ccgtgcggcg aggcggcaga ttctgctcat ccaatggtcg
    39721 ggctgaggct cgatccactc cgtcccgagc gtatcggcga aggtgtaccc gctgactgcc
    39781 agttccgggg tgatgatcca actcgctcct gaacaggccg ccctggtgat ggcctgggtg
    39841 aggaggtggc ggttttgcgc gatcgctccg ggaatcgggg cgagatgtaa gcaggcgatg
    39901 cgaagtagag atccggccat cgggtcccac gtcatgtggt ggctgtgcca atactgttgg
    39961 gcgtggagtg taccatagcc cgggtacctg agcctctccc gggtgctgtg acttgtgtcc
    40021 gcctcgttcc cctgcgtaga gtggaacgaa ggagctggcc ggcatgaatc atcctatcgt
    40081 cccgccgtca cgaaaacggt taggtcagtg gctcctgctg ctggtgcttc tgctggcgct
    40141 cctctgtgtg gcgacgatcc tggcgttgcc atggctgctg aatcgtccgg cgagcatggc
    40201 ggccttgctt cagcaggtcg aagcgcggac agggcatcgg atttccttcg agcaatcgca
    40261 tgtcgagatc tttccctcgc cccgtgtgac actgatgcag ccccggctct acgccgccac
    40321 ctctcccgat ccactgctgt tcgcggagcg ggtggaggtc gcgctgcagt ggctgccgct
    40381 tctggaggga tcccttgtcg tcaaagatct ggtcatcgac cggcctcgtg tgaccctccg
    40441 ccggtcgcca aatgggaatt gggcgctggg caacgagcat tcccccggcc ctgattcaga
    40501 gcccaccccg ccgtttgcgt tgttgcaggt cgtgaggaat ctgcttgtgg ttcaagggat
    40561 ggtgacgctc gtcgatgaat cggcttcccc gtccacttct ctcaacatca tcgtgacgca
    40621 ggctgcgtta tcgagtgaaa tgttgggccg ccgtgcgaag ctgcatcttt caggagagct
    40681 cccgcaggca ggcgggcgcg cggcgttcct ctgggaaggg tccatcacgc aacagggcga
    40741 cgaagatgga atacaggcag aaggggatct gcgactccat cagttgaatg tgcgtcaagt
    40801 gttgtctgcc tggatgggcg acggccggat caccgatggt ttctcgcagc ccgcacaatt
    40861 gacggcgcac gtgcggtgga agccgggcgc ggcgggatat gatctgcttg ccgacgatgt
    40921 gagagccgag ttggcggatg tgtctctgca gggaagcggg gcgatgctcg gaatcgggac
    40981 ggcacaagca cgattttcat ccagcctgtc tgcatctccg gtaacggccg cccggctctt
    41041 gaatgaagtg ccttccgcct ggatgtcttc tgccttacgg gcacagtttg tcgaccatgc
    41101 cgtggaagga ttgatcaccc tacaatctct gtcgatcgcc ggagacatcg ctcctggagc
    41161 acggccacac atcaacggcc tgctggctat tcgaaatggt cgattcacgc tcagttcacc
    41221 ataccctccc gtcgaggcgc tgactgtcag tatggtgttc gatgccgatc aactgcgtat
    41281 gacggaggtg cgcgcccagt gcggacccct tcgcctggca ggccgggatt tgctgatcac
    41341 gcagtgggcg accgatccgc atatcgatgt gaagataaca ggcacggcac cggtctcggg
    41401 cctggtggag acggtccgcc gtctggatga atttcccttg ttgcgagatc aggtgaccga
    41461 cgtggagcaa ccgaccgggg atgtcgaaat gattgcccat gtgatgggac gaccgctcag
    41521 cgggcagcca ctcacgctgg tggatgccga tctccgactg cagcggggcg gggggcgcag
    41581 tgctcttttg ccgatgccgg tgcggcaggt ggacgcgcac gtcactgtca cgccgaccat
    41641 ggtggcgatc gagcatctta acgggtggtt cgggccggca gcattccaga ctcatggttc
    41701 cgtcacgatg gctgacggga aagcctattc caatgtgacg ctggcgatga gcctggaggc
    41761 gtcggagctg caatcctggt gggcggaaca ggcagcggaa cacttcgtcc ccgagctgga
    41821 cggcagcatt cgcctgcgag cggccgtgac gggtgcactt ggacacccgc gagtcaaagg
    41881 ccgcattgat ctcgcagagg ccggggtccg ttggcagaac tggttgacca agccgctgca
    41941 ggcacccgcc gccattgatt tcgaagggca ggtctctcgg gacagtcatc tgctgatccg
    42001 gcatgtcggt atcacgtttc caccggtcac cgtgacggca tcggggacgg ttgacttgga
    42061 cggggaacgg gagttttcgg cgcatgtctc gtcgggccgg atcgccgtcg cgagacttcc
    42121 gaaaggcgtt gttctggggc ccgttctagc cggcacattg gaggccaccc tcgatatgga
    42181 aggccggatg acagaccgtg cgtcctggcg cacctcagnn nnnnnnnnnn nnnnnnnnnn
    42241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42301 nnnnnnnnnn nnnnnnnngc caccctcgat atggaaggcc ggatgacaga ccgtgcgtcc
    42361 tggcgcacct caggccagat cagattcgat gaaggcacga tcaaggtgga gaacctcgat
    42421 gaaccgattc gcgacgcgtt cgtgacgctg cgtttcgatc gagaccggat tcatattccc
    42481 cgcatggcgt ttcatgtcgg cgcaagtgat ctccggatat ccggctcgat cgcgcaatgg
    42541 gccgaccagc cgaaggcccg cctcgtggtg gagtcgtctc aaattgatct cgctgctttt
    42601 gaaatgtctc ggcgccgccc atcggcgtcg gcgcgccagc gatcgtcaga ccatctctgg
    42661 acggatacaa ccctccacgc gtttctgttt gccgaccatg tctattacaa gaagttcctg
    42721 ctcaccgatc tctcgacgaa aattacgtgg gatcatggcc tcctgacggt cgaacggatc
    42781 agcggggata cgaacgaggg ccagctcgct gggcaggtca agatccgcac gaagaacggg
    42841 cagatggaac aggctcgcag caccttccgc gccagcggca ttccggttga gcgagtcctg
    42901 tcgccgttcc aagagaggcc cgcgctctca ggatggctga cgacgtcggg aaaagttcaa
    42961 gcggaatttg agcggggagt gcttctgcct gccgcgttga ccagtcgtca gccgatccaa
    43021 atcctcgttg aggaagggcg catgcatcac atcccggtgc tgtctactct gctgtctgtg
    43081 ttaaacctcc cggctgtact gcaggggcag gtcaacctcg agaaagaggg catgccgttc
    43141 gatcggctca agctggcagg gttcatcaac aacggcgtgg tccacacgaa ggaatttcta
    43201 ctcgacagtc ccgtgctcaa gattagtgga accggtcgct atgacctcct ggcggatgaa
    43261 ttcgacatgg tgctggcgac gagtccgctc ggatcctact cggcgatctt gaagcgcatt
    43321 cctctgttcg gacacctcct gtcaggggat cgccaggggt ttgatactgc tgtgttcgag
    43381 ttgaagggat cggcaaacaa gccgcagttg cggtatcttc ccgctgaatc gctgatgacg
    43441 ggtgtgaagg gaacggctca gttggccttc gatattttgg tgaatgcgat taccttgccg
    43501 caaaaagcct tctcgatggt ggaagaaggg gtgaccggag gggaggagga ggatttctga
    43561 cgcccggctc aggcaacgat gtgttacggt gcaaagtcgg cccgaatgtc cagatcgttc
    43621 ttgacgcgcc ggaccccttt cacctgccgc gccagttcag ccgctctcgc cttccgttct
    43681 gccttgtgca ccgtaccggt cagggtcacg atgccctcct cggtcgtcac gatgatcccg
    43741 gagagtcccc cctgctgatc ctcggcaaac ttcgcttcga cattcgtgga aatgaccgca
    43801 tctttgggac catccggcag cggcgaggtg ccggtccaca ggcatcctgc cgaaaggcac
    43861 agcagcagcg ggagaaacag gaacgaccgg tggactaggc gcatgggctg tcgcaggtat
    43921 cgcgtctcca taaaaatcgt cccggatctt ggcatagtgc ggctccggtt tccatgccga
    43981 gttttgggat tgcgcgtcct gttcgcaacg ggcatcatgc ggcgccagga atcagtatgt
    44041 tggagcacga gggcagatgg cagagccggt ggcggatatt tgtgtaatcg gggcgggcgc
    44101 ggctggattg gcggccggga tttttgcggc tgaacgtcaa ccggctctca gagtggaagt
    44161 gttggatggg gccgcgagcg tcggcgcaaa aattcttgtt tcaggcgggg ggcgctgcaa
    44221 cgtcacgcat gatgtcgtca caccggaaga ttttttcggt acccggcacc tcgtccggaa
    44281 tgtgcttgcg gcatttccag tgaaagatac cgtcgcgtgg tttgcctcgc tgggtgtcga
    44341 actcaagcgt gaagagaccg ggaagttgtt tccggtgacc gataaggcca gaacggtcct
    44401 cgacgcgctc ctgcggcgaa gtcgcgaact cggtgtggtc cttcggccgg ggcatcgcgt
    44461 cgtcaacatt gagcgcagga ccgaatcgga agagggcggc acagaagcgc ggcccgggtt
    44521 tgtgatccgc catcagcatg gagagacacc tgcacgtcgc atcatcctgg ccaccggcgg
    44581 tcggtcgttg ccgcgcactg gaagcgacgg agggggatac gaactggtac ggcatctggg
    44641 acatcacgtc acaccgaccg taccggcatt ggtggcgttg atgctggatg aggactgttt
    44701 tcatgcggag ctttcaggct tgtcacagga ggtggagatt cagacgctgg tggaagggcg
    44761 atcggtcgac cggcgtagag gcagtctact ctggacgcat ttcggcatca gcgggccagt
    44821 cgtgatggac gccagtcgtt tctggtgtct ggcccgctcg cgaggtgaag cggccgaggt
    44881 catgggtaac ttcctgccag gccggaatgc ggagcaggcg agagaatggt tcctcgcgca
    44941 ggcggcccag aatcctcgcc ggtcattgct gaaagtgctt gcggagtcat tgccggagcg
    45001 atatgccgag gtgctttgcc gtcacgccgg gagcgatccg caacaggcct gcgctcaggt
    45061 gccacgggct attcgggacc ggctgctgga ggccctcacc cgctttcgtt ttcctgtcgt
    45121 gcgtgatcgc ggctggaact tcgctgaagt gacagccggc gggatcccct tggaagaggt
    45181 caatttccgc accatggaat cgaagctcgt gcccgggttg tatctggtgg gagaagtgct
    45241 cgactgtgat ggacggatcg gcggatttaa ttttcaatgg gcctgggcga ccggccgggt
    45301 ggcgggccgc gcggccgcgg cgagtctcgg ttcgtgaacc ggcggcttga tggggaagtg
    45361 cttagcggaa ttcatacccg acgccgaaga tcatcgcttc gtcggtcttt ttccgtcccg
    45421 gcgccggttg tgtgttcagc cgcagatcat actcgacatt gaaaaacaaa ttcttgtaga
    45481 ccgtgatccg aaggccctga tcggcattga accggaaggc gttgccggca ttgatgtccc
    45541 aaaatccttc atgccgatgg aacaccttga cgcgatccgg ccacaggctg tgctcccaac
    45601 gcaggctcca gcgtccggaa ggggtctgcg tgttcggact attcgtgaaa tgttcattca
    45661 catgggcgat accggccgat aacgaaagcg tggtggtcgg ggtttcatag aactgataac
    45721 ccaagccgct accgatcgtg gaccgaagtt gcagcgcctg cagcgtgtcc ttttccagca
    45781 tgccgaacgt ttcgatgaag atctgcttgc tgataaaata attgtgtttc aagctcgcga
    45841 gggagtttct cgcggtcact tgatcgttcg cttgcccata gttgtatttg ccttccacca
    45901 gcagccgctg ccgatgtgcg tagagtgtca gtcgggcggc gctgttgaag gccttggtac
    45961 tgctgttgcc tgacgtgttg ttccctccca ccgagacggt gcctgctacc cggaagggct
    46021 caaggttaat agcggtgata tccgacagcg gcgtggacgt atcctggcct gcttcagaga
    46081 cttcgcgttg gccctgcgtg ccgtagacga aatcgcgaag gacatcccgc gattccccgg
    46141 gaaccagaat tttgatcggg cttgtcgctg tgaggcgctg gacctttttc caatcgacct
    46201 taatttcgcc gccatagtcc gtttgaatcg tcaggagatt ttcttccatc ttgacgatcg
    46261 tgccggtgag tcggtcgccg ttgagtaaat aaatttcatc agcagccgcc ggcgtggcaa
    46321 gccacaaact aaagagaagc aacgcccgct gccagagcag gagactgaag acatggcgat
    46381 ggtgcatgga tgtaaaccgg aactcagacg ggcggcggct cgaagtcgag gcgaggtgct
    46441 gccatctgta gaccaggtct caccctaatc gagaggagaa gaagtttcaa gtgcacgtgc
    46501 gagaaagtga tctggcgtca accagcgggc ggagattatt tcgcgatggt gatgctgggc
    46561 acgccgcttc gtgacacttc cgtgacggtc atctggaaca gttggtagag cagcttgaag
    46621 gcttcacggt tccatcgagc cagtgggccg gtgatgttga cggagtaata cttgccatgg
    46681 gactgcacgg tgagatccga ttcggacggg ctggactctt gtaccaggag gtccatggtt
    46741 ttgactggat tgtccataaa aggcggcgtg cggttatctt tctcgaccgc gtattccggt
    46801 tcttcttcca cggattgcgc cagaaaattg agcatggtgt tgaagctgcg cagacggaat
    46861 tcgcccttca gcggccactc tccgccataa tgtcccggac gaatgtcgaa cgacacatcg
    46921 ttcatggggc ttcgttcagc ttcctcgtgc agccggaccc gttcctcttg cggaagtgtg
    46981 ttgggatcgt agttcgtgat gagaattctg ccggacaccg gtttacgcag ccggtaggtt
    47041 ttttgctgcg gatcgtaggc aaccagatag tcttgctcca gtgctttgaa cccctctgcc
    47101 gtcacggatt cggcgggaat ggtccacgtg cgttcaaaca gcatcgattc tgcatagagg
    47161 cgattggcat cctgaatggc ggagaggtgc agcaccactt ttcggaacat ctcatagccc
    47221 gccttatcgg tcgggctatt tcgataggcg atatcctctc cctgatcatc cacccgcagc
    47281 tcttttgcca tcagacgaag caggagatcg atatcgccgc cttgtcggag caggagcgtg
    47341 aacttggttt cttgaaaggg tgtcagcagg cggcgagtga attcttcccc gtcgatcggt
    47401 gcaatgctga tagtgggatt ctccgccaca ctgccgccga acaccggcag cagcgttttg
    47461 ccggcttctc cggtcaaagt aggagtcgct ccggcgttga tccggaaatc gaaggtggcc
    47521 gcgacgtttg agacgcctgt aaagtgaacg ggctgatggt gatgggcgcg cgcgatattt
    47581 aacagcagct gtttcgaaat ggagtcggtg atggcgttat cgtaggagac gactgcgcgc
    47641 gtgagcgtcg gcggcgagag acagccagca aggttcatga agacggtaag agcgatcagg
    47701 ccacgcagca aaagggatgg aatggtcata ggcatcgagt tcggtgatga aggtggccca
    47761 tcaacagcgg ttctagcata cctattccag gaagcctagg gggatggatg ggcatgtgtt
    47821 tcgccggacg gcgcgagctc gcctcggggc ggcgatttgg tttatcatcg ggcactgtga
    47881 tgaccaggaa cagaatgaga gctcgacaca tgaggcattc cgtgtgggtt ggggtgatga
    47941 cgctgctgtg cttcaccgga tgcggcagtt cggcaaagct catttccggg ctgagcatcg
    48001 aagaattgaa acccgtgccg ggtattctgt cgagtgccag gcttttaaaa gggacgatcc
    48061 gcagtatcac cggcgaccgt gtggaagtgg acaccggcga acgggccccc cgttatttgt
    48121 cgctggaggc gggcggtgat gaagcccgcg ggttgcaggt cggcggggcg gtggagatta
    48181 tggtcaatga ccatgacgac atcgtcgcgt accagcggcc ggccgagacg ccgccgacca
    48241 aagtgtttcg cggatctctg ggcaaggtgt ttaatccgca aaaagaacgc gtcacgattc
    48301 gtctcgatac cgggcgcgat gtggggttct acgcgcggtc cgtcgcgatg gagaaactcg
    48361 cggcgctgga ggtgggagaa acggccgatt ttgcggtgga tcgcgcctat ctgctcgtgg
    48421 atgtcttgtc gctggggaag cctcgcgacc tgggagtcgg gtcttcgctg cccgcgccac
    48481 aacgtcgcgt ggaaggggcg tttgttacga tcggcgacgg ccgcatgcgg ctgaggatga
    48541 agatcgacga cattcaatcc tttcccgtgc gtcccttgtt gcagccggcc atcgagcaac
    48601 tgagcccgaa tgagaccgtc tcgatttacc tcgacgggca ggggcaggtg atcgatatgg
    48661 tacggacggc ccgccggtaa gctttgtcag cggcgccggg gaagcttcac gatacgggta
    48721 atgatccaat cgttgaggcg atcgggcagg aggagtgcca gccatgagcg aatcgtggca
    48781 tctgagccca ccaggtatcg agtatgtggc cagcgggacc gcagcgcatg cgccaccgcc
    48841 tcagccacca ccttcgccgg ctgagcctgt tcccctgctg ctgtggccgc ctctttgatg
    48901 cgggtgaacc cttcctggta catgtccttg aggtccggac tccaggtcgc atcccaggta
    48961 tcgacttctt ttctggtctt tccccaaatg ggcgtggcaa tggcgcctgg cgtaatgagg
    49021 gcgacgcgga tgccccatgg ttgcagttca aggcgcaacg catcggtgat cgcttccagc
    49081 gcatgtttgg aggcggcgta gggggccatg aacggcatgg aagctcgtcc ggcgatggag
    49141 cccatgttga tgatgcgtcc gtgacccgtg cggatcaacg gcaaaaacgt ctgggtcacc
    49201 gcgatcagtc cgaacacgtt cacctcgaat tgccgtcgcc aatcggcgag gggcaggagt
    49261 tcaatgggac cggccacgcc gatgccggca ttattcacca ggcctgcaag gccgcgatcg
    49321 cctaccatgg cggcgaccgt atggctcgcg gctgcgatcg acgcagagtc ggtgacatcg
    49381 agacgaatgg gcatgagccg tggaccggct tgccgctgca gacgttcgcc gtccgcggga
    49441 tgacgaacac aggcgaagac gcggtacccg agtgtgtcca acgcgagggc gcaggcggct
    49501 ccgatgcccg atgaggcacc ggtgatgacc acggagtctg acggggaaga ggttgaaaac
    49561 cagttgagca tgtctcgcat cccttgtgtc tggccgcgta ccctaccata ctttacggtg
    49621 tgaccctaca atcagatgta tccgatgacg tgcgagaggc gctgtgcctt cggcgtagaa
    49681 caagcaaacg ctcgagcgat gagaggagca ggcatgcaca cagagaaatg caaatgggcg
    49741 ctgctgctta tgatgggact gagcctggct gcatacgtgt ctcccctgaa cgcaggcacg
    49801 gatcagaagg gagtcgtcgc cacggagaag cttgtcacgg tgcagccgcg ggaaatcgac
    49861 gacgtcttag acaatcccgg gatgggattt gccgactttc acttcggctt cggccatcct
    49921 ccgaagcctg atgaatatcc acatacgacc gtggcctatt tccgttggcc ctgggcagag
    49981 ctggaaccgg ccgagggcca gtacaatttt gcattggtgg acggagtcat cgcacaggcg
    50041 aaggctaagg gtgagacgct cgccatccgg atcgtctccg aatataagac cggcaccccg
    50101 caatggttgc tggataaggg agtggccagc atcaaagaaa cggacgggat tttccccgac
    50161 tataacaatc ccatcttcct cgactaccac gaacgtctca tccgcgcgtt cggcgagcgg
    50221 tatggccggt cgctcgacat cgatcatgtg gatatcggat ctgtcgggtg ttggggggaa
    50281 tggaatacgg cgtgctgtgt aggagcagag acgcagtgta aggcctacta cccaactgaa
    50341 gcgaatcagc tcgccattac cgattggtat cttaaatatt tctccggaac tccccttgtg
    50401 atgcttcacg gaggacaatt gcaatatgcg gcctcgcgag gggccggttg gcgggctgat
    50461 tgcttcggtg actacggcta tttctctccc gactggaacc acatggagca tgcctatgcc
    50521 ccagtcctgg aggcgcaggt agtggccgat gcgtggaaac aggggccggt acagatggaa
    50581 gtctgcggct atatccatga atggtacgaa cggggcttcg atctcgaccg catcctcgag
    50641 aaggggttgg aatggcatgt ctcggtgttg aacgccaagt caaaaccggt acccgccgcc
    50701 tggcggccgc gcttcaatga gtttctgaaa aaaatcgggt atcgatttgt gctgcgtgag
    50761 ctgacccacc cggcctcgat cggagccggt gaatcgctca tcattcactc acggtgggac
    50821 aataaagggg tggcgcccat ctaccatgcc tggccgctgg cgtaccggtt gcgttcgggc
    50881 acggatgaag tggtcgcgca gtggaccagc gctgccgatc tgaagcggtg gctgccgggg
    50941 ccttcgcacc tggctgagga tgcggtggtg gtgccgagca ccgttcctgc cggcgtctat
    51001 acgctcgatg tcggaatttt atctgaggat gcgaagtctg cgacggtcga gttagcgata
    51061 gaaggcaaac ggaccgaccg ctggtacgcc gtgtcgcgcg tggagattcg ataggctgtc
    51121 ggccgtcgtg cagatgggtt gatctgggaa gagctgagga tgcggaacgg cgacttgggc
    51181 tcgacctgca tctctagaca gccagggccg gggccgccgc ctgctcgagg cggagtaccg
    51241 tcatcacctg gttgccgttg ccgcggtatt cgatctgatc gaagctgact ttgttggcca
    51301 tggcgattcc tcgtccgtgg gtatcaaagg cgcgctcggg atcgaagtcc atgtatcttt
    51361 gccaatcaaa cccgtttccc tgatcggtga tgtgcagcga cagcccctgc ggaaggcgag
    51421 aaaattttgc cgtggcgaca cgcgacgagt aggggggcga gctgagccga tgagcgattt
    51481 cttcttccag cttatcttct tcgatgaggc gtgacttttc cttatacccg atgctcaggt
    51541 tgccgtgttc gatcgcgttc aaggtcagtt cgaggatgcc ggtgacgacg cggtcggcat
    51601 cgggataggc atgcgcgagc aacatggcga ggtttcgtgc ttcttcgggc gttctaaagt
    51661 ggaatgtccc cgactccaac aggcgcatgg tcgtcgtagt ccggcgcaga tcctgccgga
    51721 tgtccagata gctgatgtgg tcgcggaccg cggcatcnnn nnnnnnnnnn nnnnnnnnnn
    51781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51841 nnnnnnnnnn nnnnnnntcc caggcttgcc ggccgtcttg ggctgtggtg cagcgatatt
    51901 gacgttcggg aagaaactct ctcagaattt tgaggttcag agtcgagtca tcgaccagta
    51961 acagttgcgg ttgagggaaa atcataatat ggagcacctc cgcttggcgg ccaagccgtc
    52021 ggaagaaaaa gcatagagtc tggcctcaca tcgcagaaca aggccgtgat atgtctatcg
    52081 gaagtctctg aagaatactt aagtgaagaa ggaaggaata tcgcgatgga ctccgccggg
    52141 gtctaacccc aggcggagtc atcagagagg tctattgctt cacgatatca agcagttcca
    52201 cttcaaacac cagggtggcg cctggcttga tcaccggggg tgacccacgg tcgccgtagg
    52261 cgatgccgga cgggcagacc agcttgctct tgccgccgac cttaatcaac tgtaccgctt
    52321 cggtccaaca cttgatgacc tggttgagcg gaaacgtggc cggttctcca cgcttgacgg
    52381 agctgtcgaa gacggttcca tcggtcaggg tgccatggta atggactttg accgtatccg
    52441 tggctttggg ggtcgcgcct tttccttcct tgatcgtggt cacaatggcg ccggattctg
    52501 tctttttggc acctggctcc gctgcggcct tggccaggaa tgcggctccg gcttttttct
    52561 cgacatcggc cagggcattg gaccggacct gctgcatctg ctgaatcttg ggcccgtaca
    52621 cgttcatgtc caccttctgt gaccgcttca gcaccccatc ggcgatgccc gacttgacga
    52681 actcaagttc ggcttcgttc aacgtaaagg gcgtgagtga ttggctgatg gcgacaccga
    52741 gggcatacag cgttttttgc tcgtcgttgg ccggttcgga ggcggccgcc caactgagcg
    52801 aggagatgag aagcaccact gcggaaacgg caccagtcac atgacgcata gacgatcctt
    52861 tctgctgaaa cagcatgact gagcccacca tatgacaggc agcgtatgcg gctcaagata
    52921 attcttgggc aggcggctga aa
//
LOCUS       DAOQDS010000025        52853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_203_length_52853_cov_21.414069, whole genome shotgun sequence.
ACCESSION   DAOQDS010000025 DAOQDS010000000
VERSION     DAOQDS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 52853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52853
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203_length_52853_cov_21.414069"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..72)
                     /locus_tag="PK589_07945"
     tRNA            complement(1..72)
                     /locus_tag="PK589_07945"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37..39),aa:Lys,seq:ttt)
     gene            complement(188..1510)
                     /locus_tag="PK589_07950"
     CDS             complement(188..1510)
                     /locus_tag="PK589_07950"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNJ19573.1"
                     /translation="MANLVIIGSQWGDEGKGKIVDILAKDADMVVRYQGGSNAGHTVI
                     NKRDTFIFHLIPSGILYRGTRCLIGNGVVVDPGALIEEMDHLQGQGVKIGKNFAVSDR
                     AHLILPYHKAIDKASEQSKGVRRIGTTGRGIGPSYGDKMARIGIRMGDLLNPPLFKQK
                     LEENLVDINWLLEQLHKVDCFELEKVYQQYMAYADRLKSYILDTTMVVNKAVDAGKTV
                     LFEGAQGTHLDVDFGTYPYVTSSSSSAGGACTGTGVGPTKIEAVMGITKAYTTRVGSG
                     PFPTELTDEVGGWLQERGKEFGATTGRARRCGWFDSVVVRHATRVNGLSSLAVTKLDV
                     LDGRNELKICTGYRVNGKLHRDMPSDLLALTNCEPVYERIRGWSAPTTGVTTYKKLPV
                     EAKRYLTRIEELAECRIDMISTGSRRDETIILNNPLKAGRRKRAPRSR"
     gene            1984..2059
                     /locus_tag="PK589_07955"
     tRNA            1984..2059
                     /locus_tag="PK589_07955"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2017..2019,aa:Ala,seq:ggc)
     gene            2219..4060
                     /locus_tag="PK589_07960"
     CDS             2219..4060
                     /locus_tag="PK589_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ19574.1"
                     /translation="MLQLESVHKQYSTKVLLEGATAHLRPGARVGLVGPNGAGKTTLF
                     RMILGEESPDKGTIRKRPRLRVGYLPQELETIVGKTVLDAAHRDIYPEHEAERILSGL
                     GFTEADFSRPIENLSGGYRMRVALAHLLLSNPDVLMLDEPTNHLDKPTQRWFERFLLE
                     SNLTLLVISHDTKFLDGIATHIWELRDRTLQEYRGNYTKFQELRAARDAQIEAAANRQ
                     SKEVARVQSFIDRFRYQANKAKQVQSRIKQLDKVKLIERQRDTKRVRFKFPLPAASGR
                     HVLELKGVAKSYGEKVIYRSLNFAVERGQRIALVGENGAGKSTLLKMLAGVLPFEKGS
                     RHVGHGVTLHYFAQHQAESLNQDHTILESLAEVSAQAETNFLRGIAGAFLFSGDDQKK
                     PIKALSGGERNRVALARMLVEPANTLLLDEPTNHLDPASVDMLTDAMTDFPGTIVFIS
                     HDPTFLARVATMIVEVDEGQAKTYLGDYEYFLWKKAQEFESIKESSAELAAAQNDKSS
                     GPTKAMASQVQQKSSGGERRDLNKTQARLEKQVSRAESEIASTESKVKARELELADPA
                     LYKELGRWSELQQEQETWKKELERLTARWESLSEELQGVREKLTALG"
     gene            complement(4057..4434)
                     /locus_tag="PK589_07965"
     CDS             complement(4057..4434)
                     /locus_tag="PK589_07965"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ19575.1"
                     /translation="MARQTNSTESVSERRKYVRATLVGSALVSPQNGAKGFTAVLNNV
                     NKIGAGLHSKETLPIGAKITVSLAFLDPDRVEQQEKLSATVAWAKPWEKGMLLGVVWD
                     DLVTKEKNRWLYYYLEETLKSTD"
     gene            complement(4476..5795)
                     /locus_tag="PK589_07970"
     CDS             complement(4476..5795)
                     /locus_tag="PK589_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNJ19576.1"
                     /translation="MPAHDSTGDLFASSQPAPRITPSGKVPLAERLRPRSFDDLVGQD
                     DVVGPGRPLRSAIEQDRLSSVIFWGPPGCGKTTLAGLVAQYTKSQFVPFSAVTGGIPE
                     LREIIKAAEQRRTRGMATILFVDEIHRFNKAQQDAFLPHVERGTVVLIGATTENPSFE
                     LIAPLLSRSLVIVLQPLTTEALGSILDRAVADTDRGLGSLRLTIQPAARDRLIAFGNG
                     DARSLLTTLEFVAGQAPVGMDGRRIIDESVLEAALLKKALRYDKGGEEHYNLISAYIK
                     SLRDSDPDGALYWLARMLEGGENPRFIARRMVIFASEDIGNAEPQALVVANAVAQAVE
                     FVGLPEAQITLAHGTTYLASRPKDNASYVGLQEAIRDARQHGNLGVPLHLRNAVTSLM
                     KEIGYGQGYRYVHNDPAAKSEQGHLPEPLKGKRYYRPRTAQDHEERG"
     gene            5942..6751
                     /locus_tag="PK589_07975"
     CDS             5942..6751
                     /locus_tag="PK589_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNJ19577.1"
                     /translation="MFFQTQDIRLAYDDRGSGVPLVFLHAFPLNRSMWAPQVAALSTQ
                     FRTIALDFRGHGESDAPLWSFSLEQYADDVAALLDHLNIPQAVLVGLSMGGYVSLAFS
                     KKCGDRLCALVLADTRAQEDSPEGKRGRFHLAQTAYTQGTEAVANTMLPKLLGATSLK
                     SKPELVEQIRRTIEHTPVSGILVDLMAMAERSDSVAHLTAISCPTLVVIGDEDHTTSL
                     VDAQIMASHIPNARLAVIPAAGHLSNCEQPELFNDLLRGFVEGVRANGRRS"
     gene            complement(6748..7383)
                     /gene="folE"
                     /locus_tag="PK589_07980"
     CDS             complement(6748..7383)
                     /gene="folE"
                     /locus_tag="PK589_07980"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNJ19578.1"
                     /translation="MAKVVSGSRARRSRTTATPTADDPIEGLMTKLLLELGEEPGRNG
                     LLNTPKRVAKAMRFMTQGYRQDIDHLLNGALFPIEYDEMVIVKDIDFFSMCEHHLLPF
                     FGKCHVGYLPNKKVVGLSKIPRVVDAFSRRLQVQERLTVQIAETLKKKLNAHGVAVVM
                     EARHLCMMMRGVEKQNTVAVSSSMLGVFRTQQQTREEFLKLIRGSSVKDGG"
     gene            complement(7376..7825)
                     /locus_tag="PK589_07985"
     CDS             complement(7376..7825)
                     /locus_tag="PK589_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNJ19579.1"
                     /translation="MSEATITRRYRFCAAHRLHTDQLSSEENWTTFGKCNNPNGHGHN
                     YVVFVSVRGDMDSVTHQVVDLSKLDEVVQRIVVRRFDHQDLNKDPEFTTQTTTGENLV
                     LLIWNLLVNEVPGGRLVKVGVIETRDNYFEYSGSAKQVRREDGVRHG"
     gene            complement(7961..8287)
                     /gene="erpA"
                     /locus_tag="PK589_07990"
                     /pseudo
     CDS             complement(7961..8287)
                     /gene="erpA"
                     /locus_tag="PK589_07990"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
     gene            complement(8346..8702)
                     /locus_tag="PK589_07995"
     CDS             complement(8346..8702)
                     /locus_tag="PK589_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNJ19580.1"
                     /translation="MPRVSFLHPEGRSGEVESNLTLLEAAKALGFGLNHDCGGNASCT
                     TCRVEVQMGGENLSEIDFDEQDLLDREALSEPWHRLGCQARVLGDVVVRVPETKWVQP
                     TSASSSQFGKKGAGLP"
     gene            complement(8715..10016)
                     /gene="nuoF"
                     /locus_tag="PK589_08000"
     CDS             complement(8715..10016)
                     /gene="nuoF"
                     /locus_tag="PK589_08000"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNJ19581.1"
                     /translation="MTLPQPRVLQKLDGAPWEIDPYLKTGGYDAWRKCLKELTPADVV
                     GEIKKAGLRGRGGAGFPTGIKWDKVLNHRVPERYFVCNAGEHEPGTFKDRHLLKHIPH
                     QLIEGCLIASRTVNAKASYIYVNHEYEEEEANLRKAIAQARERGLLGKNILGTGVDLD
                     LEVFSGHGSYVAGEETAMLESMQGRPAMPRQKPPFYPTDFGLYGKPTLVNNVETLCNI
                     PQILKNGAAWFTQVGTEKCPGTMLFSLSGSINRPGVYEMPMGVTIRDLIETCGGGVPN
                     GRKIKAVFPGGPAFSMVTADQLDLPMDFDSLKKAGTGLGSAGTIVIDDATCMVAATLK
                     YSNFFKGESCGQCPPCRMGTINLATLVEKIERGEGSQKDLDSLLQLCGFVKGTGYCTL
                     ITGAAVLVQSSLRLFQHEFEEHIRMHRCPFQSAQTGVGAKA"
     gene            complement(10270..10857)
                     /locus_tag="PK589_08005"
     CDS             complement(10270..10857)
                     /locus_tag="PK589_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HNJ19582.1"
                     /translation="MKFLEDPYQTLGAGVALAVGLIVVYLGMAGVGAGEADWFGLVVR
                     WVHFLAGITWIGLLYFFNLINAGFLKSLDGPTKNIVIPKLMPAALNWFRHGATVTVLA
                     GIVLYGYLYSKGGTGALALGIGGLLGIIMMGNVHGIIWPNQKKVIAAVAAAAQGTPAP
                     PEMAQWGKTALYASRINFLLSIPMLFFMGAGSHLK"
     gene            complement(10961..11902)
                     /gene="mdh"
                     /locus_tag="PK589_08010"
     CDS             complement(10961..11902)
                     /gene="mdh"
                     /locus_tag="PK589_08010"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNJ19583.1"
                     /translation="MGRPKITVVGAGNVGGTAAQRLAEKNAYDVVLVDIVPGIPQGKA
                     LDITQAGPVCGYSTRVVGTNGYDETAGSSIAVITSGIPRKPGMSRDELLGTNAKIVKT
                     VVQELVSRSPNIILILVTNPLDAMVHVARKVSGLPKSRVLGMAGVLDTARLRSFVAEE
                     LDVPGTDVQAMVLGGHGDTMVPLVRQTTVAGKPITDRLSSERLAALIKRTQDGGAEIV
                     GLLKTGSAFYAPSASVVDMVEAIMKDQKRVVPCAMLCEGEYGLKDVIVGVPVRLGRGG
                     GESIVEYDLTVEERTALQASSDAVRDLCGVVDRLLTA"
     gene            complement(11975..12385)
                     /locus_tag="PK589_08015"
     CDS             complement(11975..12385)
                     /locus_tag="PK589_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19584.1"
                     /translation="MIEQSPRPTVPPWLYKLFTGHQYPYVRRQAKFANRDFKPGEERP
                     EPTREAIDAKFWEIYPRCTAKILQEVKAGMIVVFHELGEYPPGGYQDLVDAPEEFLAA
                     KYGKKKIKVNFYDGDNFVCTINFKVGGWTGHEPA"
     gene            complement(12429..15464)
                     /locus_tag="PK589_08020"
     CDS             complement(12429..15464)
                     /locus_tag="PK589_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ19585.1"
                     /translation="MAPGHSFPQLIERLADQLGPASATTMVDAIVASATRPDAPAGVW
                     LLLEELTDAAPKAARGAIEALLELQRRGLLAEVLPWLDLGVAIAADSGALALRFFKES
                     PLLLGLLDPPQRAVVLAAGLELADRQANVAVEFIRVAPEVVPVLPPADWPTWMELACE
                     LSETDFALAVEYIRQIPAIARVLSLDTVRAWMQFGMTLIVDNSLGKPDYLGALEFFRT
                     SPGILADIPDQISRHQVIRVGAQIAERSPEMGIALLSEAPSILRGLPTDEWRRQVLQY
                     GLILAERDAETALAYMRRCPELVALIGSGEGAQQKFQSWFASGMDVLEYSVEGGRAYF
                     GMESEKALGALSQAMSGVPLRQIARRVKLFAQALCGRDLRIYDLPDSLESAQPMPRAT
                     VSDDGRSIGLPSLVRRHSTYEENVRLYMVMAAHEAGHLEFGTFDLPLARLEDVSKELT
                     QRYGRPALREVRTLGDMFARYPQPGLIRDLWALVEDARVEYLLRREYPGLSRDLAVIA
                     KDAVQVRSLAHGMTVRELVVDQLLLLSTVEAGSMPLPDALKREIMHLWSLCQTIFHPQ
                     ATAEEAVRLADRLYVSMDELLAIRREAIPSPDEAESPEQSIPAPKASEDLSDTYRPMD
                     NWDYRGAMDPNLVRDRTESTPEQSPASGQGDSEGGAGLAGDSGGSSGAGTARSQQMSQ
                     EMLVPGRRQPSLVEEMLAVEGEGARPQDDQTETVKAVRYREWDAAIQDYRMNWCRVVE
                     REAVEGSSDFVEGTLAAQRGPVALLRRYFESLRPPGLRRVPGQVDGEDLDMDAVIGRL
                     ADIQAGIEPSERIYVRREKREREVAAAFLVDLSGSTSRQVERGRRIIDIEKQGLVLLC
                     EALSAIGDQFAVYGYSGQGRHLVDFVVVKDFDQLVTGRAGAKLGGMVPLQQNRDGAAI
                     RHATTKLLARQAKTRLLVMISDGRPLDDGYKDEYSLEDTRMALREARASGIEPVCITI
                     DKQADPYLRRMYGDVRYVVIDRVEGLPEKLPRIYHRLTA"
     gene            15596..16042
                     /locus_tag="PK589_08025"
     CDS             15596..16042
                     /locus_tag="PK589_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HNJ19586.1"
                     /translation="MAEPSKESLEKMWKYVKGFAEKSGTAMHPNPAVTNAVVLGLAAH
                     VDELGKPLCPCNFYPDKQAEAKLRRWMCACDEMQIYKYCHCLLFVNEEGLPITEYLPE
                     DHEGRQCYGLVPDPTPGKGRALRHKALPMAPKVPESPATPDSSAQS"
     gene            16120..17286
                     /locus_tag="PK589_08030"
     CDS             16120..17286
                     /locus_tag="PK589_08030"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNJ19587.1"
                     /translation="MAGCLHTMEALDPRALPVTTAPAQRLQSYVSFLASPALAGRQPG
                     TPGNRQAAQYIEEQFRLVGLQPLASLEGYRQTISDRMGDNVIGVIPPIDHTARTRWIV
                     IGAHYDHLGYPFLGADDNASSIAILIETARNLTGLSRHGIMFVAFNGEELPYFGTAEM
                     GSQFLFHHLPPEVGAAENLQAAIIMDLMGGVHWAPLKDAIFATGAEKSPALYRRVTET
                     RVSPLTIYRGGIHLVEEIPDHGHKAFSDYDVFRNQKVPFLFLSAARSPRYHTAGDVAS
                     TLHYERMAATVEWLRQLLALMSQDEAPYAFDAHRVEFADEVASFTSLVEQALHEETRI
                     PGTSRWSLRKLEQDLEWLREVRGSAPTPQQLERLERISIRVQCLMADYSGCFTF"
     gene            complement(17325..17810)
                     /locus_tag="PK589_08035"
     CDS             complement(17325..17810)
                     /locus_tag="PK589_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19588.1"
                     /translation="MHGEPGVELWPRSSVLRKIRSIIFRWSLSLIVVVVSGCAGPEPI
                     FKSTTYLQLHGQQQAQDEAAVCAVKAERAGLQHGTNRSGNAGAGAALGVIGGAAVGAS
                     AGLVGGPTGIAIGAAAGGAVGGILGLFAGTYKPLHPQQDYAAFVERCLKEKGYETEGW
                     Q"
     gene            complement(17866..18201)
                     /locus_tag="PK589_08040"
     CDS             complement(17866..18201)
                     /locus_tag="PK589_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNJ19589.1"
                     /translation="MTTHNSGRESKSSAAAQVVRTTPEGAVISVHVQPKASRTQCAGL
                     HGDAVKIRVAAPPADGAANEELCRFLARTCGLPVSGVEILSGTSSRQKRILLRGRSAE
                     QVRAQLQLE"
     gene            complement(18247..19086)
                     /locus_tag="PK589_08045"
     CDS             complement(18247..19086)
                     /locus_tag="PK589_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNJ19590.1"
                     /translation="MSEGRAATAPKGQAQDIDHYRIAREPFYADVRGEVGLFTIAAQS
                     RMPVMLKGPTGCGKTRFVQHMAYRLGRPLITVACHEDLTASDLVGRYLLKGQETVWMD
                     GPLTLGVKQGAIVYLDEIVEARKDTTVIIHPLSDDRRLLPIEKKGQVIEAVDEFMLVI
                     SYNPGYQSILKDLKQSTKQRFMAIEFDYPLPDIECRVVAHEAGVSPEIAQRLVKIAEK
                     VRNLKNHGLEEGVSTRLLIYAATLIKQGVPADRACDVAIARPITDDPDMLRSIIEVVK
                     AIF"
     gene            complement(19181..22282)
                     /locus_tag="PK589_08050"
     CDS             complement(19181..22282)
                     /locus_tag="PK589_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ19591.1"
                     /translation="MTASPLPGFPRRSYPWLPVLIVGVTIVALVIGVVMLRSIEVRMV
                     EATGENLTLASAEIADKLDRLLFERHADVRMMARAFTDRSADKKYINEYLQWMKETYA
                     PVYLWLGVTDPHGRMIAATDSSIIGQDYGRSGWFDRVRQTHTVQVDEVEIHESNHKIE
                     SVAFTAPILSVSGQFLGAVTTRIGLPMLEDVLIETVREIQQTDGAAGPFEYQFLTKGG
                     LVFVDSSSSNIDRVNLRELNLPSVLRSSSGKAGYVEEIHVRRHVPVVTGYSQTKGYSD
                     YSSLQWTILLRIDRDVILLPIRAMMWKLGIAGVVVWLPMVGLLLWATGRLREQHRQAQ
                     QESEWARAAEAALLQSQERNRVIVDTALDAVISMDAGGIITDWNAQAVNIFGWEREEA
                     LGRRVSETVIPVRDRDGHERGLRHFLETGQGALLNRRIEMMGSHRDGHEFPVEITISP
                     ARLGDAYIFSAFIRDITARKRTERQLASQYAVTRVLAESRTLEEAGPKILQAICESLE
                     WELGIFWRLDRQGSVLRCLDVWQSPGLNAEEFVLDTWRHTFALGKGLPGRIWQAGEPT
                     WIKDVVQEANFPRGGAASRVGLHGAFGFPVRVGTEVEGVIELFRREIKQPDEQLLKMV
                     ADVGLKIGQFGERARAEDALRRTEAQLQQSQKMEAVGRLAGGVAHDFNNMLTVIRGYS
                     ELVLSRLSPTDGFRKELEEVKKAADRASGLTGQLLAFSRRQFIAPKVLDLNSVIHNME
                     GMLRRLLGEDIVELCTVLDPGLGQLKADPGQVEQVIMNLAVNARDAMPDGGRLTVETA
                     NVQFGQRRNRPPMGAEPGSYIALIVRDTGHGMDEDTQSHVFEPFFTTKEKGKGTGLGL
                     STVYGIVKQSGGFIEVESKPGRGATFKIFFPRVEAVSEETESVAVGGDSVRGRETVLL
                     VEDEPGVRRLVNETLRLHGYTVLEARHGIEALLTGAKHAGPIHLLLTDVVMPQMSGPE
                     VAEKLATVRPEVKVLYMSGYPDHPAFSKGGIDTEHSFLQKPFTPTTLAQKVREVLDGS
                     KVS"
     gene            complement(22279..23787)
                     /locus_tag="PK589_08055"
     CDS             complement(22279..23787)
                     /locus_tag="PK589_08055"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNJ19592.1"
                     /translation="MTSPLVPVSPANPTRPVGQSLLQIVPSTTCLHCDVCCRFPEVDS
                     FLRPFFTGDEIRAAVAAGLTPELFPTTEGTQIDLVPNPAGEGYVCPAFDSETSHCRIY
                     EVRPLDCRLYPFALMWDAERTHVLLGWDTKCPYMRDAPSSLVDQAVDGVAQWVEADER
                     VDTLARYPRLIGRYQDDVIPLRTLARVTERVQRAKPQIRRQALTIQDRGRIEAALTVT
                     AGFQESPLAAASFAYHYIWKHRLTYTWAELQGHLCLFAESPDGIFLVLPPLGSGPIDQ
                     PTAEAFRLMRAWNGDSSVTRMENVPESFVPALRASGYETGEKEPDYLYAAADLVDLAG
                     EAYRSPRAACNRFVREHGGVLEPYDCRDQTACVSLFREWREQKLRSGSDEWANALLED
                     AVGAHETALSAHSELGLTGAVVRVGGRIRAYTMGLWLNQSVFCVLLEVADRDIVGAGA
                     FVFREFCRQAHAKGACWINTMDDSGLPSLAKAKRWYHPARLLPNYVVTEFRR"
     gene            24007..25521
                     /locus_tag="PK589_08060"
     CDS             24007..25521
                     /locus_tag="PK589_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19593.1"
                     /translation="MALAQHSREPLNSDEARRRCLAIRDALVHAGVYGPADTEQGTAT
                     AGSGVSPVRFRLSPTPFPLSSSEVMCFQHLGQDLLAFYRALNRLYQESARGIQPGWVA
                     AYLDQGKPESLVTFSRMNRFRNLIPGIIRPDVIPTADGMVITELDSVPGGIGLTACMT
                     RAYDDQLGLSPSPASPPSERSPLIAGRDGMLQGFAAMLRAQQGDREGCLAIVVSDEAK
                     EYRPEMEWMAQRLRDIGFQAFCVEPRAVRCTDEGLYLQDEHGMRTIGLLYRFFELFDL
                     KNIPKSELMMYAAKKGQVAVTPPYKPALEEKLAFGLFHHPELEPFWKETLTAETRTNL
                     GQLFPRTWILDPQPVPPTAVIPHLLISGRAVSDFRRLAHTTQKERQLVVKPSGFSELA
                     WGSRGVSIGHDLPQVEWAAALEQALQSFSTTPYVLQEFHKGRLFEASYWDERSDTVVP
                     MSGRVRLSPYYFVAGDKAELGGILATLCPANKKIIHGMVDAIMAPCAIAPGVAA"
     gene            25591..25836
                     /locus_tag="PK589_08065"
     CDS             25591..25836
                     /locus_tag="PK589_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNJ19594.1"
                     /translation="MALTFYHVRWCPDCAVVRDRLSELNLSYEDVVVPDFRPMRKQVY
                     EVSGQYYVPVLKDGDTVLTETHDILAHLQTHYEKAQP"
     gene            25951..27084
                     /locus_tag="PK589_08070"
     CDS             25951..27084
                     /locus_tag="PK589_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNJ19595.1"
                     /translation="MEDWKRILAQSVVKPKDLAERLGVDPKEVEDIVGDYPMRITPTV
                     LATIKEKGDAIWKQVVPDRAEMADADAEDDPLEEDLMSPVPHLVHRYPDRVLLMVTNQ
                     CPIYCRFCTRKRLVGKPGFLKKGELDRAIAYLREHQEVRDVILSGGDPLLLPDHLLER
                     ILKSLRTIPHLELIRIGTRVPGSLPERITPKLCEIIKKYHPFYMNLHFNHPDELTPDV
                     KRACGMLADAGVPLGAQTVLLKGVNDDPEVMKRLMHQLLLARVKPYYLYQADLTKGTN
                     HFRTSVETGLKIIKSLQGHTSGMAVPHFVIDAPGGGGKIPLLPGDYLVNLDEDGAVLR
                     NYENNTYHYPQPGSPQGRELPMVGAHPSWAASGNGCDGGGEHL"
     gene            27081..28232
                     /locus_tag="PK589_08075"
     CDS             27081..28232
                     /locus_tag="PK589_08075"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HNJ19596.1"
                     /translation="MKRAASSKGILPYQHITQLITRGAIKSDAPIEDRQIQPASLDLR
                     LGKKAYRLISSFLPELSAISSRLNVLDFYQSDLVMYEMDLTQGAILEKGHVYLVPLLE
                     HLELPATLRARANPKSTTGRLDVFTRVVTDLNAGFDEIRAGYTGPLYLEVVPRSFAIK
                     VRTGDCLNQIRFVRGDATVSDTALQKLHAADPLLFRNASSPKPLPQKEFRTERGLFLR
                     IDLTGSAHEEAVIGYRAKKNSHVIDLAKIGHYAALDFWEPLKRHRHDSLLLEPEEFYI
                     LASKERIRVPSGYAAEMVAYEAACGELRTHYAGFFDPGFGYGDGTMRGTQVVLEVRPH
                     DVPFLIHDGQTFFKVVYDRMLTVPSQVYGTALGSSYQRQALTLSKHFKA"
     gene            28235..28642
                     /locus_tag="PK589_08080"
     CDS             28235..28642
                     /locus_tag="PK589_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19597.1"
                     /translation="MPPPSDLPDPSTRPSWPPPHRPDDESTSERQAHVFGMMIGTALM
                     FIGFLDVFLSISGGFEINVVPLLIYFSGIAVWANAVVENPTVRYSLMAGSVLISLAFF
                     HYGEVLFWHKQVVFWSTVAIVMYFMFKDPKPTA"
     gene            28687..29403
                     /locus_tag="PK589_08085"
     CDS             28687..29403
                     /locus_tag="PK589_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNJ19598.1"
                     /translation="MTIAVIMPVLNESRCLAHILAHTQALGFDEVIIVDGGSSDDTFS
                     IAQSFAKQCDTDPLRASGSMRVLTAPRGRARQLNTGAAASACDVLLFLHADTLLPSNA
                     RRAVLTALSDAAAVGGRFNVRFDSSRLTALVVSSLMNVRSRWSGIATGDQAIFVRRAV
                     FETMGGFADIPLMEDIDFTRRLKRTGPLVSLSDEVVTAFRRWEQQGPLRTILLMWTLR
                     FLYWIGVNPHRLQHFYSMVR"
     gene            29407..30159
                     /locus_tag="PK589_08090"
     CDS             29407..30159
                     /locus_tag="PK589_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNJ19599.1"
                     /translation="MSPQPSDRKQPASAALVIFAKAPVPGQVKTRLCPALTEDEAATL
                     HGSFVLDTLERTKAAASTFKLSFDRILACAPSSTHVFFRIMEARYGVTLFDQEGDDLG
                     ARMQHTFAQLFARGYRHICLVGTDVPSLPLTHYRDAFAALTRHDLVLGPANDGGYYLI
                     GMTRPHPKLFAHIPWSTDQVLALTQQNAAAEGLNTALLPSWNDVDTLEDLQALIEDCV
                     ADKKRPKKERIYSNRTAGTLELLAKRLRERRG"
     gene            30171..30905
                     /locus_tag="PK589_08095"
     CDS             30171..30905
                     /locus_tag="PK589_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ19600.1"
                     /translation="MPNQVALITGGAKGIGRAIALDLAAQHWCIAICYRTSAAAAEET
                     SAAIMARGGQALALQCDVSDPAASKQLVAQVEAQWGRIDALINGAGPYHRVNLFDETT
                     TGWREMFDGNLHPIFYLGQAVAPGMKARKHGRIINFSMANADQMVAQPEVTGHYIAKA
                     GVLILTRTLAKLLAPYGITVNAISPGFIDSGSAPAEELAGVVKRIPAGYVGSVNDTVA
                     AVRYLLSEEARYVNGANLHLSGGWGI"
     gene            complement(30907..32481)
                     /locus_tag="PK589_08100"
     CDS             complement(30907..32481)
                     /locus_tag="PK589_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNJ19601.1"
                     /translation="MIPSGIPPVLFRKRASCLRPDEAPYHPVPGISCETALKPIESFL
                     SLMGENHSAKSPLCVDLDGTLIKTDLLWESLLALLKQNPLSIFLLPVWLLRGRAYLKH
                     QIAQRVTLDASTLPYDQELVEFLSKERLAGRELILATASHVSFAQVVATHLGLFDGRV
                     FGSDSSVNLKGARKVALLVERYGTRRFAYAGNSTADFPVWAEASEAIVVNASAGVASR
                     ARTLTTVSRVFRAPVNLVKQVAKALRVHQWSKNVLVFVPGVASHQLANGWLLLQASLA
                     FLSFSLCASAVYIVNDCLDLESDRRHPRKRNRPFASGNLSIPFGLALAAVCLIGGVLL
                     ALALPSVFLLVLGGYLALTTGYSFYLKQFVLVDVIVLAQLYTVRVYGGGAATGVPPSH
                     WLLTFSLFLFLSLALVKRFTELRLMSQVEGKGLHGRGYWVTDLEHISSIGTASGLLAV
                     LVLALYISSKEVLLLYSHAEVLWLVCPVMLYWISRVWMLAYRNRMDDDPVVFAVKDPK
                     SYAMAGIIAAILFFAK"
     gene            complement(32509..32838)
                     /locus_tag="PK589_08105"
     CDS             complement(32509..32838)
                     /locus_tag="PK589_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNJ19602.1"
                     /translation="MADPIEEEIQREIQANKILIYGKGTKTMPMCGFTRETMHFFEKY
                     GYPYELIDVLSQPAKREALTKLTNWPTLPKVFIDGQFYGDTDILDPMEAKGEVVPLLK
                     KAFGAES"
     gene            complement(32849..33094)
                     /locus_tag="PK589_08110"
     CDS             complement(32849..33094)
                     /locus_tag="PK589_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNJ19603.1"
                     /translation="MITAEAVTTYIRQMFPDAAVTVIDKTGTQDHLIVRVISTGFADK
                     NLLDRHRMVYQALAGPMKDGRIHALEITAMTPDEVKK"
     gene            33259..33621
                     /locus_tag="PK589_08115"
     CDS             33259..33621
                     /locus_tag="PK589_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19604.1"
                     /translation="MSQTKSRQPRPLVQTTLFVLVLVLGCQATLSVRTASAAGSGSEA
                     IQGLGSYFLTLPYGGIKMAVAVLGAVAGGMGFIFTGGDKATVDKIWGPAIGGEYVVTP
                     QHLRGEKDLHFFGNPPAK"
     gene            33906..34340
                     /locus_tag="PK589_08120"
     CDS             33906..34340
                     /locus_tag="PK589_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ19605.1"
                     /translation="MGEFKSGFFMSETACNGRVLVVDDEPDIRKVVRMTLQKAGYEVI
                     EAENGEKAIEAINTGENRLLLDVLICDIRMPKINGVEAIAYFQQEWPRVPIIVLTGFP
                     DTDMATSFLRSGVVDYLVKPVEGEKLRTAVARAMEQRELAQL"
     gene            34456..35505
                     /locus_tag="PK589_08125"
     CDS             34456..35505
                     /locus_tag="PK589_08125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ19606.1"
                     /translation="MTLTSTKIAIIGAGRGGTALLELLHQIPDVDIVGIADKDPAAPG
                     LQRAKDLNVPVEEQVYDLIRNHGVNLIMDVTGDPSMAPFIQAMKRPGAEVLSGATTRL
                     LWKLVQHQSKLEAELFQADKLAGLGSFAAGIAHDINNPLQLILGLAENLTDEQDLTVV
                     HEQARDITEAVKRTSAICRDLTRYARRNGTGEDIMVNLNTKLDEALRIARYAVTLQDV
                     TVVKRYAEDASVLGNPDELLHVFVNLITNAVQAIQDRGTLTLHTNVGPDGQVSASVSD
                     TGCGIPKEAFSKIFEPLYTTKPPGKGTGLGLYNVMSVISKMEGHICVESDVGVGTTFR
                     IEFPQVQPTTSCAPL"
     gene            35502..37979
                     /locus_tag="PK589_08130"
     CDS             35502..37979
                     /locus_tag="PK589_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ19607.1"
                     /translation="MTITWRPFSNPLGWGLKRKLIVSMLLVGVVPLLLGLSMAFLQGS
                     KEIQVVSGESFQALATEAARKLDLLVAEEVARTSRIATHPEIIRQLEHHRDLLQTADK
                     TLSATTITQQRTRWAARDPIAVKAITENRTAVLLRSFYAGAQNESDLLLPQVVRAATK
                     KLFITDVQGNLFAALTTFPSFSNADSPWWKGTYNKGVGQLYIEDLHFDEQAKAYVFSI
                     SLPIMDSLKYEVVGVLHRVIDAKEFFSPSTHPIRFGKTGHVMLIDSRGIVLSCPILPT
                     GVRLSDPALIPQVTVREPGWVTADSDGHGGQGTAIIGFALLPETSRATNGALEVGAWH
                     TFVWQSSDELFSPIRHLMLWMLILASVALFLLATLGYFAASRIVTPVRRLQEAARLIG
                     RGELQEPIQIRTGDELEDLAVEFNRMHKQLEASFAGLSTQVEEKTQEVQYLQKATDQV
                     LDAVPTPIIMVDQQGLVQYMNKASREAVQADSWSSRPLFDLLPVDEEHRQALQRDFRA
                     VEEGMSLAPVRHDAPPLLKPARDPLNQTIGPAPASSRRELRIGPHLYQYEWFPLESRS
                     GAGKRFGLVLRDTTDESRLQDQLIQAEKSGSLDVLTAGIGHELNNPLFGILGLGEAIQ
                     EEGDLARAKGYAQDIVGHGRKMAAIIRDFTGVATRESKSQRMPVDVTAQLEQALAIVQ
                     TSHECLGLNVQKHYVPVSPVTALPDQLRLAFINVLTNAIQAMNGQGNLWLTTEEQDDV
                     ITIKIRDNGPGIPKQHLGKVFDPFYTTKGQGEGSGLGLTVAQRLIKKFGGEIRMESPP
                     EGQGTTCVITLPAEKAAVRKEEPCIST"
     gene            38006..38557
                     /locus_tag="PK589_08135"
     CDS             38006..38557
                     /locus_tag="PK589_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNJ19608.1"
                     /translation="MALFLACVSTYSLQAKDLTPPAGISPEKVADFVHAVLDADRTIY
                     TNQVVNRMQAKGIVSAMEHWEHENALPLPAQFLQHSGKLVAESGRGIRYRLISLWPIY
                     QRNAPATDLERQALESFAQTPDRPFTGIVTSGRKQFFQAVYADRAISAACIKCHNSHP
                     LSPKRDFALNDVIGGMTITIPLD"
     gene            complement(38580..39662)
                     /locus_tag="PK589_08140"
     CDS             complement(38580..39662)
                     /locus_tag="PK589_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNJ19609.1"
                     /translation="MNRRQIFAACFFGVLLALLYQMALMFRPFVFPVLWAVILAHLCF
                     PLHIRLTAWLGGKESHSAVLLTLAALALVVVPLVVLSVMLVKEAGSAERAVREWIASG
                     GVQQLPDRLSGLPGGSVAREWLERFTGQESDLEQFVLSSAKTFSKFIVGELSDLVRDV
                     FMLVADFLVMVFTLFFFFKDGRHWLASLYGLIPLEASHKDKILVRLDQTIRAVVKGIV
                     LTAVAQGLLAGAAYAVLGVPFPMVLMALTILLAPLPFGGTALIWGPVAGYLLWSGTVG
                     KGLVMLAWGIGVVSTIDQILKPLFIGQGAQIPVLLLTFSVLGGLAAYGILGLFLGPIL
                     VGLFLTALQIYRDEYQLQPASPIVRS"
     gene            complement(39837..40277)
                     /locus_tag="PK589_08145"
     CDS             complement(39837..40277)
                     /locus_tag="PK589_08145"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNJ19610.1"
                     /translation="MMKKPGFVESDNITLLAKGVLLRGEIHVEGTVRIDGRLEGDLKT
                     SGTVVIGEDGVVQGTITAGTIISSGKIKATVRATERIQLLKTGLLVGEVHAPAFSMED
                     GAKFQGLSDMGVTSWGDDAQKLPGNVREIASQRPKAVAMAGENA"
     gene            complement(40364..42661)
                     /locus_tag="PK589_08150"
     CDS             complement(40364..42661)
                     /locus_tag="PK589_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNJ19611.1"
                     /translation="MRFVRRWLLRALVAVTGILLLVLGSALAYGLYLSASLALPAGDE
                     HPPRLIYGAPFLLKPDLDVVSSHLIERLSRLGYQAVDTPVRAPGEFRVSPGQIDIYLH
                     EFSDLHLRPMPARLLLEQGRVTRVLSIQTGDELFPAYLEPQLISGLRGASRQVREWVS
                     YDDLSARFLDILLAIEDRRFFSHPGIDPIAVGRAVWTNVTKGTVIQGGSTITQQLAKN
                     LFYSPQRTFGRKLKESIAALVLETKYPKKAILESYANEIYLGQVGSVSIYGVGEAAHR
                     YFGKRVDTLSLEEAALLVGMIKGPNTYSPLRNAALAKQRRDVVLGRLHEQGLINDEAW
                     AQAIKTPVQVMPPQDTLADAPFFVDYLLRQVEEATGSPLPDGVRAYTTLDPVLQRLAT
                     QTLESELIKLETTYPALKGHPTPLQGALVAIDPKTGGILAMVGSRDYRTSQFNRAVQA
                     KRQPGSLFKPLVYLAAMEARRELNGGQPVTAATSIVDEPVTFESATGRWTPQNYDHKF
                     HGTVSLRTALEQSLNIPAVKIAQLVGTKRILSLAEKLGIRSPLADNLSIALGTSGVSL
                     LEMTSAFGVLAHDGVSMTPTALQAVMTSEGDPAWHHTPARHQAVSPQAAYVMTSLLKG
                     VVERGTAAKAKAMGLRGIVAGKTGTTDGYRDAWFIGYTPDVVVGAWVGFDDEDALHLT
                     GAQAALPMWVEFVRQILPAAPRDFVMPPAVITRDIDPQSSQLATSKCPQRSAELFIEG
                     TEPSVYCEVHGSGMWERVKRSFGFF"
     gene            42841..44304
                     /locus_tag="PK589_08155"
     CDS             42841..44304
                     /locus_tag="PK589_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNJ19612.1"
                     /translation="MSTIRFCTAPASSETDPSSASRETVAPGRNARTTARLLLLTGAL
                     LAAWSTIAPGSVLAGSFRIYDHSASATGQASAFTAQADDASAGYYNPAGMTQLRGVQL
                     SAGTTLIAGGFSFQNGAGTQANGDLRGSVAVPPPSNLYITANLKDLGIGSSDSTAVGL
                     AVFSPFGTLTRWPDSSPFSTATTKAAFELIDIRPSIAFRPLPDLAIGLGADVYTFSGA
                     FGEGQVERQFRWPGGLGIPAGTGMELNGRDTAAGWNASLLYTPLRNEDGKPLVNIGLI
                     YRSQATLHLDGQLLAGGTHIADARTTFVVPQVLTGGIALWPVRNKEREWKLELDVDYT
                     GWKSVRNLDSHLSNGITVATPANWISGYTVMIGTEHKWLNPASLPDWDIALRAGYWHS
                     QKAIPDQTFNPAIPDGDNHAVSTGVGFMCRDNGRFLGVIPCGGASKLSPKGLGIDLAY
                     QAILYESRTVAGNVNPTVNGTYRTTLHVGSLNLRVNF"
     gene            44419..>46079
                     /locus_tag="PK589_08160"
     CDS             44419..>46079
                     /locus_tag="PK589_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNJ19613.1"
                     /translation="MDRPAHTDPALELQSQQVSVLYRNMPTGVLASAVNAAILAAVEW
                     SVVPHTPLLVWVTILWIVAGARALLIYNYRRTTPATWSSADWHRWMLVATTMAGVGWG
                     GSVYFLTPEIPLAYELVQLFVLGGMTAGAVSVLSVSFQLFLCYALPVTLPTLGHLFFS
                     GYDFHVIMGVMGTLFLLATIHSSWTFNHVLLSSLRLQLEKLSLVQSLTTRSEAVERLN
                     RDITKEIHDRRVIEEQLRTAQSDLEHRIAERTADLAHANSRLQDEVREHRRTEGARLS
                     SEKRFNYLTDNLNQGVWFAQAQPPQVLYVNPAFERIWGLPAARFYENPKLWRDCVHPD
                     DQRMVTEVYDTELTRPTGRDVQLLYRIIRPDGQTRWIHDRMVIHRTETGEVDSLSGIT
                     EDITDARELEDQLRQAQKMEAVGRLAGGVAHDFNNVMTVILGYSAVLLQELSQNSSAR
                     YFVQEIQRAGERCAALTGQLLAFSRKQMLHPVSLDLHRVIRDLMALLKSLIGEHVTIV
                     LQLDPSPRWVKADAVQLEQVLLNLAVNARDAMPHGGTLTIDTCQVL"
     assembly_gap    46080..46179
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <46180..46562
                     /locus_tag="PK589_08165"
     CDS             <46180..46562
                     /locus_tag="PK589_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ19614.1"
                     /translation="GTEIILLVEDEPSVRLLTQHILRTHGYTVHEAEDGFQAMDLIRR
                     NNLHIDLLITDLVMPGMNGKELAMRLRSHFDDLKVLYMSGYSDNLPVTGGESQGQTTF
                     LQKPFSPEDLIRLVREILHSVSPA"
     gene            complement(46559..48049)
                     /locus_tag="PK589_08170"
     CDS             complement(46559..48049)
                     /locus_tag="PK589_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNJ19615.1"
                     /translation="MLLSFVILYLACSVGIGLYAATRVHNTKDFAVAGRSLPLPVVTA
                     TVFATWFGAETVLGISATFVKDGLRAVVADPFGSSLCLILAGLWFARRLYRLNLLTIG
                     DFYRLRYNRAVEVLCTLCIVASYLGWVSAQIKALGLVFNVVTDGAMSQSAGMMLGAVI
                     VLTYTTFGGMFSVAILDFVQITIIMGGMLYIGSVISGLAGGVGTVVTHAAAAGKLDFF
                     PPAQIEAWIPFVGAWVTMMFGSIPQQDVFQRITSARNEDTAVRGSVLGGTLYFVFAFV
                     PMFLAYSATLIDPAQVAGLLERDSQLILPTLIVQHTPMAAQIIFFGALLSAIMSCSSA
                     TLLAPSVAFSENIVKSLMPTISDRGLLAVMRSVLVGFTALVLLFALNSEASIFKMVES
                     AYKVTLVAAFVPLLAGLYWPRATTQGAICAIVTGLATWLMLEGTVTPDQVWPPQLVGA
                     LMAAGGMVIGSLLPQWITHPATIDVPVGHRSHSMPVESHTGTHPSP"
     gene            48269..48487
                     /locus_tag="PK589_08175"
     CDS             48269..48487
                     /locus_tag="PK589_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19616.1"
                     /translation="MRYSLYFAWVLAVLSICILTTLASFPVLTNGERHPEPRITAGPE
                     KLRTIKIHRLRCNPFTSSCQLSRQKSQH"
     gene            complement(48515..49465)
                     /locus_tag="PK589_08180"
     CDS             complement(48515..49465)
                     /locus_tag="PK589_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HNJ19617.1"
                     /translation="MGEKQVIAVVGATGMQGGGLVRAILANPASGLAVRALTRDVNSD
                     KAKELARLGAEVVAADVHDVESLKRAFSGAAGAFCVTFFWAHLSPEKEYAEAEAMAKA
                     AKAAGVRHVIWSTLEDTRRWVPLSDNRMPTLMGKYKVPHFDAKGEADQLFKQLGLPTT
                     FLLTSFYWDNLIHFGMGPKPGPDGALAFTLPMGEARLPGIAADDIGKCALGIFKKREA
                     YLGKTVGIAGEHLTGSQMATALGKALGREVRYNAVPPEVYRNFGFPGADDLANMFQFK
                     RDFNAVFCAAREPAIARALNGGLQTFAQWLEVNKARIPLS"
     gene            49631..50137
                     /locus_tag="PK589_08185"
     CDS             49631..50137
                     /locus_tag="PK589_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19618.1"
                     /translation="MVRLTTHRLESPRQWWTSLAMGCSVLLFALPVFAAESSGPWECS
                     NYTGDAHTRCLQAFIEIQREKISKLEAEVQLQQSTVGQLKDQADRQNAMTAELQRQMS
                     QQSSAPSTYGYVSPGIAPGLLYGYPSVGFGLYLGRPWIYGSPFYARPYAWGPRYYRPY
                     YGRWHRRW"
     assembly_gap    51239..51338
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <51339..51797
                     /locus_tag="PK589_08190"
     CDS             <51339..51797
                     /locus_tag="PK589_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19619.1"
                     /translation="LGLGWTHNLGARRVESVSVTNRIVRIDDERNDLVRAMPEVHLWI
                     DRWDEQRWSWGPFLAVAPGSRIIDAVGGGLMVGYRPHRSDQYSFNFGIGGILDLDTQV
                     LGDGLVANEPLPPRETSARTKHTTGAGLLVLFSVGWDLSAPHQVSPPGRK"
     gene            51893..52837
                     /locus_tag="PK589_08195"
     CDS             51893..52837
                     /locus_tag="PK589_08195"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNJ19620.1"
                     /translation="MTPQPQFPNEHTESGEFQRQEDAFRQWVTDDGRSGLPAAAGRYH
                     LYVSLACPWAHRTIIVRALKQLESVVGMTVVDPIRDEQGWAFRTGQGHSIDPINGFHF
                     LSEAYHLTDPTYRSRVTVPVLWDTVTKRIVSNSDDDLMRMFNAEFNRFTISTLDLYPA
                     ALRADIDEMNTFLYEQVNNGVYKAGFATSQRGYEQAARSLFAALDQLDTRLSQRRYLF
                     GPQFLESDWRLFVTLIRFDAVYHGHFKCNVRRIIDYPNLSGYLRDLYQVPGIADTVNL
                     DHIKRHYYVTHDDINPTQIVPIGPQLDLESPHGRDKFS"
BASE COUNT        11366 a        16342 c        14342 g        10603 t
ORIGIN      
        1 gagtcggctg ggattcgaac ccaggaccct cgccttaaaa gggcgatgct ctaccagctg
       61 agctaccgac tctcccaaga tggagacgac acatcgtcac gcgaggatcg tgacgggagg
      121 gatcttacca ctgacgcgtc ctgtatcgct acggcgcaca tcggggaaga cgcaaaaaac
      181 acagcgatca tcgtgaacgt ggcgctcgct tgcggcggcc tgccttgagc ggattgttga
      241 gaatgatggt ttcatcacgc cgtgaaccgg tagagatcat atcaattcta cactcggcca
      301 gttcttcgat cctcgtgaga taccgcttcg cttccaccgg caatttctta tacgtggtaa
      361 caccggttgt gggtgcgctc cacccacgaa tgcgttcata caccggctcg caattggtca
      421 atgcgagcaa gtccgacggc atgtcacgat gaagcttgcc gttcacccgg tagcccgtgc
      481 agatcttcaa ctcgttccgg ccgtccaaca catccaattt cgtgaccgcg agagaagaca
      541 gcccgttgac tctcgtcgca tgtcggacca cgacactgtc aaaccacccg cagcggcgag
      601 cccggccggt cgtggcaccg aattcttttc cgcgctcctg gagccacccg ccaacttcat
      661 cggttaattc ggtcgggaaa ggtccgctgc caacgcgcgt cgtataggcc ttggtaatgc
      721 ccatcaccgc ctcgattttc gtcggcccca ccccggttcc cgtacaggcg ccgccggccg
      781 aagaactcga cgaagtgacg tagggatagg tgccgaagtc cacgtccaag tgcgttccct
      841 gcgcgccttc gaacaggacc gtttttccag cgtcgacggc tttattgacg accatcgtcg
      901 tatcgaggat gtagctcttc agtcgatcag cataggccat gtattgctgg tagacctttt
      961 ccaactcgaa gcagtccact ttgtgcagct gctcaaggag ccaattgatg tcgacgagat
     1021 tttcttccag tttctgcttg aagagcgggg gattcagcaa atcgcccatg cgaatgccga
     1081 tccgcgccat tttgtcgccg taggacgggc cgatcccacg cccagtggtt ccaatacgac
     1141 ggacaccttt agactgctcc gaggctttat cgatggcctt atggtacggc aggatcaaat
     1201 gggcacgatc gctgacggcg aaattttttc cgattttgac gccctggcct tggagatgat
     1261 ccatttcttc gatcaatgct ccgggatcaa ccacgacgcc gttaccgatc aggcaccgag
     1321 tgccacgata taagatgccc gaaggaatga gatggaaaat gaacgtgtcc cgtttattga
     1381 tgaccgtatg gccggcattg gagccgccct ggtaccgcac gaccatgtcg gcatccttgg
     1441 ccaggatatc gacaattttc cctttacctt cgtcgcccca ttgggagccg atgatgacga
     1501 gattcgccat tgttcgctgc ctctgctctt tcttcccgtc tgaactcgaa aaaaaggctc
     1561 tgacctcgta ctgatcagag ccggaagggc atcatggtag gattcacacc gcggtttgtc
     1621 aagcagggtt ttcgagaaca agcttccgcc cttggttgcg tcacctccag cgtgcttcct
     1681 aagacggata ggctgggtat tcttcctccc gacgccgcat tctcttagac ggcggatgat
     1741 cctggatctt ctccgccgct tccttgctct cacaaggcgg catgtaccgc gccctttaca
     1801 ttgcgcgcgt ccaacgaggg ccttctgagg ccgcgcgttg cgcgagcaca aaagggcgcg
     1861 gcacatcgcc gcctcaccac ccttttcttg actgacaaaa tccgcccgtg ctagattgcg
     1921 gagttacgtc tgcagcagca ttcgttttct cgctgcctgt gcacataaga tccaagcttg
     1981 cgtggggctg tggcgcagtt gggagcgcgc gtgaatggca ttcacgaggt cgccggttca
     2041 atcccggcca gctccaccaa aacaagcttg tccgaccaac actccgtgat gtgtgcgggg
     2101 atatccgaac tctacgagtc atacgcttag ccagccgccg cttcacacgg gggcgctgac
     2161 actccgcagc gggtacctcg ccgaacgttt cacctcagac cccgagtact actaatccat
     2221 gcttcagctt gaatctgtcc ataagcaata ttccacgaaa gtgttgctcg aaggggctac
     2281 cgcccatctg cgacctggag cccgcgtcgg tctcgtgggc cccaatggcg cgggaaaaac
     2341 gaccctcttt cggatgattc tgggcgaaga gtctcccgat aaaggcacca tccgcaaacg
     2401 cccgcgactc cgcgtcggct acctccccca ggaattggaa actatcgtcg ggaaaactgt
     2461 gttggatgct gcccatcgcg acatctaccc ggaacatgaa gccgaacgta ttctttccgg
     2521 gttagggttt accgaagcgg acttctccag gccgattgag aacctctccg gcggataccg
     2581 catgcgcgtg gcgctcgcgc atctcctgct ctcgaacccc gacgtgctca tgttggacga
     2641 gccgacgaac cacttggaca aaccgactca gcggtggttc gaacggtttc tgctggaatc
     2701 caacctgacg ttgctcgtga tcagccacga caccaaattc ctggatggca tcgccacgca
     2761 tatctgggaa ctccgggatc ggacccttca ggaatatcgt gggaactata cgaagttcca
     2821 agagctccga gcggcccgtg atgctcagat tgaggcggcg gcgaatcggc aaagcaagga
     2881 agtggcgcgc gtgcagagct ttatcgaccg cttccgctat caggccaaca aagccaagca
     2941 ggtgcagtcg cgcatcaagc aattggataa ggtcaagctg atcgaacggc aacgcgatac
     3001 caagcgggtg cggttcaagt ttccgctgcc cgcggccagc ggccggcacg tgctcgagct
     3061 caagggcgtc gcgaagagtt atggagagaa ggtcatttac cgatccctga atttcgcggt
     3121 ggaacgcggc cagcgcatcg cactggtggg tgaaaatggc gccggcaaaa gtacgctgct
     3181 caagatgctg gctggcgtat tgccgttcga gaagggatcc cgccatgtcg gccatggagt
     3241 gaccctgcac tactttgctc agcaccaggc cgaatccttg aaccaggacc acacgattct
     3301 ggaatcactg gccgaagtct cggcgcaggc tgaaacgaat tttcttcgag gcatcgccgg
     3361 agccttcctt tttagcggcg acgatcagaa aaagccgatc aaggcactca gcggcggcga
     3421 gcgcaatcgg gtggccctgg ctcgcatgct ggtggaaccg gccaataccc ttttgcttga
     3481 tgagccgacc aaccacctcg atccggcctc ggtggatatg ttgacggatg ctatgaccga
     3541 ttttccgggg acaatcgtgt tcatttctca cgatccaacg tttctcgccc gcgttgcgac
     3601 catgatcgtc gaggtggatg aaggccaggc caaaacctat ttgggcgact acgagtattt
     3661 cttgtggaag aaggcgcagg aattcgaatc gatcaaggaa agcagcgcgg aacttgccgc
     3721 cgcccagaat gacaaatcgt caggtcccac gaaggccatg gcgtcgcagg tccaacagaa
     3781 gtcttccggc ggagaacggc gggacttgaa caaaacccag gcacgcctcg agaaacaagt
     3841 ctcgcgcgcg gaatctgaga tcgcctctac ggaatccaag gtcaaggcga gagagctgga
     3901 actcgcggat ccggcgctct ataaggaact tggccgctgg agcgaattgc aacaagagca
     3961 ggagacctgg aagaaagagc tggaacggct cacggcccgc tgggaatcgt tgtcggagga
     4021 attgcagggg gtacgggaaa aactgacggc actcggttag tccgtcgact tcagcgtctc
     4081 ttccagatag taatacaacc agcggttctt ttctttcgtc accagatcat cccacaccac
     4141 gccaagcaac atccccttct cccacggttt ggcccaggcc accgtggcgc tcaacttctc
     4201 ctgctgctca acgcgatcgg gatccagaaa tgccaaggag accgtgatct tggcaccaat
     4261 cggcagggtt tccttcgaat gcaatcccgc gccgatcttg ttcacattat tcaaaaccgc
     4321 cgtaaatccc ttggccccgt tctgtgggga gaccagggct gacccgacca gagtggcccg
     4381 gacatacttc cggcgttcac tgacactttc ggttgaattg gtctgacgcg ccatagcgac
     4441 ctcggtgcta agtataaccg tataaccagc tttgtctagc ccctttcttc atgatcttgg
     4501 gccgtccggg gacgataata ccgcttgccc ttgagcggtt cagggaggtg gccctgctcg
     4561 gatttggccg cagggtcatt gtgcacatac cgatatcctt ggccataccc gatctctttc
     4621 atgagcgacg tcacggcatt acgaaggtgc aacggcacgc ccagattgcc gtgttggcgc
     4681 gcatctcgaa tggcctcttg aagtcccaca taagacgcat tgtcctttgg ccgggatgcc
     4741 aggtacgtcg ttccatgagc caaggtgatc tgtgcctccg ggagcccgac aaactcgacc
     4801 gcttgcgcca cggcattagc cacaactaac gcctgcggtt cggcattgcc gatgtcttca
     4861 gatgcaaaaa tcaccatacg tcgcgcgata aatcgagggt tttctcctcc ctccagcatg
     4921 cgcgcgagcc aatagagggc cccatccgga tcggaatcac gcaggctctt gatgtaggcg
     4981 gagatcaaat tgtaatgctc ttcgccaccc ttatcgtagc gaagggcctt tttcagcagg
     5041 gcggcctcca acaccgattc atcgatgatc cggcggccat ccatgccgac cggcgcttgc
     5101 ccggccacaa attccagcgt tgttaagagg cttctcgcat ctccatttcc gaaggcaatc
     5161 aatcggtcgc gggcagcggg ctgtattgtg aggcgaagcg agcccaaccc acggtcggta
     5221 tcagccaccg cacgatctaa aatcgagccg agtgcctcgg tggttagcgg ctggagcacg
     5281 atcacgagcg atcgtgaaag caagggggca atgagctcga aagacggatt ctccgtagtc
     5341 gcgccgatca gcaccaccgt tccccgttcg acatgcggaa gaaaggcatc ctgctgcgcc
     5401 ttattgaatc gatggatctc gtccacaaag agaatggtcg ccatccccct cgttcgccgc
     5461 tgctctgcag ctttgatgat ctcgcgtagt tccgggatcc ctcccgtgac cgcagagaac
     5521 ggcacgaact gcgatttcgt atattgagcc accaagccgg ccaacgtggt ttttccacaa
     5581 cccggaggtc cccagaagat cacagaagaa agccgatcct gctcaattgc acttctcaac
     5641 ggacgtccag gacctaccac gtcgtcctgg cccacgagat catcgaaact tcgagggcgc
     5701 aggcgttccg ccaaggggac tttcccggaa ggtgttatcc ttggcgcggg ctgggatgaa
     5761 gcgaataaat cgcctgtcga atcatgagct ggcatcattg atctcaggtg caaggccttc
     5821 tgcattctat acgtcgccac ggaaacaccg caaactggcc cgtctcactc aaccatcccc
     5881 ttgattctca accctggcaa tgctaccacc catccggcat caattcttgg cagggggacc
     5941 gatgtttttt cagacacagg atattcgatt ggcatatgac gaccgcggct caggcgtccc
     6001 actcgtgttt ctgcacgcgt ttccgttgaa tcgctccatg tgggcgccgc aagtcgccgc
     6061 gctctccaca caatttagaa ctatcgcgct cgattttcgc ggtcatggcg agtcggatgc
     6121 ccctctgtgg tccttttcac tcgaacagta tgccgacgac gtggccgccc tgctcgacca
     6181 tctgaacatt ccgcaggctg tcctggtggg actctcaatg ggtggatacg tcagcctggc
     6241 attctcaaag aaatgcggcg atcggctctg tgcgttggta ctcgccgata ctcgagcaca
     6301 ggaggacagt ccggaaggaa agagaggacg ctttcatctg gcgcaaaccg cctataccca
     6361 aggcaccgag gcggtggcga atactatgct gccgaagttg ctcggcgcca cgtcactaaa
     6421 gagtaaaccc gaactggtcg agcaaattcg ccggacaatt gaacacactc cggtcagcgg
     6481 aattctggtc gatttgatgg ccatggcgga acggagtgat tccgttgccc atctgacggc
     6541 catttcctgc ccaacccttg tcgtgatcgg agacgaagac cacacgacat cgctcgtcga
     6601 cgctcaaata atggccagcc acataccaaa tgcccggctg gccgtcatac cggccgccgg
     6661 gcatctgagc aactgcgaac aacctgagct gtttaatgat ctcttgcggg gattcgtcga
     6721 aggggtgcgg gcaaacggca ggcgatccta gcctccgtct ttgacgctgc tcccacgaat
     6781 aagcttgagg aattcttctc gggtctgctg ttgggtgcgg aatactccca gcatagagct
     6841 gcttacggcg acggtatttt gcttctcgac tccgcgcatc atcatgcaga gatgtcgcgc
     6901 ttccatgacc acggcgacac catgggcatt cagctttttc ttgagcgttt cggcgatttg
     6961 aacggtcaag cgttcctgga cttgtaggcg ccggctgaag gcatcgacga cccgcggaat
     7021 cttgctgagt ccgacaacct tcttgttcgg cagatacccg acgtgacact ttccgaaaaa
     7081 cggcaacaaa tggtgctcgc acatgctgaa gaaatcgata tccttcacga tcaccatttc
     7141 atcgtactcg atgggaaaca gagcgccatt gagcaagtga tcgatgtcct gacgatagcc
     7201 ttgggtcata aaccgcatcg ctttcgcgac acgtttgggc gtattcagta gaccgttacg
     7261 gccaggctcc tcccccagtt ccagcaaaag cttggtcatc aacccttcga tcgggtcatc
     7321 ggcggtgggc gttgcagtgg tacgcgaccg tctggcgcgg gacccggaaa cgactttagc
     7381 catgcctcac tccatcttct ctccgcacct gcttcgcgga tccggaatat tcgaaatagt
     7441 tgtctcgcgt ctcgatcaca ccaactttca ccagacgccc ccccggaact tcatttacca
     7501 acaaattcca aatcagtaag acgagattct ctccggtcgt cgtttgtgtc gtgaactccg
     7561 gatctttgtt caggtcctga tgatcgaagc gtcgcacgac aattcgctgg accacctcat
     7621 caagctttga caggtcaacc acctgatggg ttacagaatc catgtcgccc ctgaccgaga
     7681 caaacacgac atagttgtgc ccgtgcccat tgggattgtt gcacttcccg aatgtggtcc
     7741 aattttcttc agacgacagc tgatcggtgt gcaagcgatg cgcggcgcaa aaacgatagc
     7801 ggcgcgtaat ggtggcttcg gacatccgga cgattctcca gaaagatcat caaaacgaac
     7861 aagccgggag agctacccgc tagagagggg agccactccc ggcctgatac gtcactcaac
     7921 gaaacatcgg cgttccggtt gaagcacaac cggaaggctc ttatgcactg aaggaactgc
     7981 cgcagccaca ggtggtcttg gcttgcgggt tcttaatgga gaagccggac ccttgcagac
     8041 tgtcgacgta atcgacttcc gcaccctgca acagcggggc actctgagaa tccatgatga
     8101 ccttcacatc acctttctca atgaccgtat catcatcggc catttttgat tcgaaggcca
     8161 ttccatactg atacccgtgg catccaccgc cacgcacata aattctcagg ccgaccacat
     8221 ctttctcttc ggtcatcagt tcttttatct tctgctctgc caattgcgta atcgttacca
     8281 tccgttggtc ctccttctgg tctgcatgtt cggcacgtta tgttacttta ccatctcgcg
     8341 ggaacttacg gaagcccggc cccttttttc ccgaactggc tggatgacgc tgatgtgggt
     8401 tgcacccact tggtttcggg aacgcgaacc actacatcac ctaacaccct cgcctgacaa
     8461 ccaagtcgat gccacggctc agagagggct tcgcgatcga ggagatcctg ctcgtcgaaa
     8521 tcaatctcgg acaggttctc tccgcccatt tgcacttcaa cccggcaggt cgtacaggat
     8581 gcgttccctc cgcagtcgtg attgagccca aaaccaaggg ccttcgcagc ttccagcaag
     8641 gtgaggttcg actcaacctc cccactacgc ccttccggat gaagaaagct cacgcgcggc
     8701 atgggccgac tcctctacgc cttcgcgcca acaccagtct gagctgactg gaacggacaa
     8761 cggtgcatgc gaatatgttc ctcaaattca tgctggaata accgcaggct gctttgcact
     8821 agaaccgcgg caccggtgat gagcgtgcaa tatcccgttc ccttcacgaa cccgcacagc
     8881 tgcaggagag agtccagatc cttttggctt ccctctcctc gctcaatctt ttcgaccaat
     8941 gtcgccagat tgattgtacc cattcggcag ggaggacatt gtccgcaact ttctcccttg
     9001 aagaaattgg aatatttgag ggtcgccgct accatacagg tggcatcatc aatcacaatg
     9061 gtcccagccg accccaaccc ggtccctgct ttcttgagcg agtcgaagtc catcggcaga
     9121 tccaactggt ctgccgtcac catcgagaag gcaggtcctc ccgggaaaac cgccttgatc
     9181 ttgcgcccgt tcggaacgcc cccgccacag gtttcaatga gatccctgat ggtgacgccc
     9241 atcggcattt catagacgcc aggccgattg atcgaaccgc tcagtgaaaa cagcatggtg
     9301 ccgggacatt tctctgtccc cacctgcgtg aaccaggccg caccattttt tagaatctgg
     9361 ggaatgttgc acaaggtttc gacgttgttc accaacgttg gcttcccgta caagccgaaa
     9421 tccgtcggat aaaacggtgg tttctgcctg ggcatcgccg gacgcccttg catcgattcc
     9481 aacatcgccg tttcttctcc cgccacgtaa ctgccgtggc cggaaaatac ttccagatcc
     9541 agatccacac ccgtgccgag aatgttcttg cccaacaaac ctcgttctct agcctgagca
     9601 atggcctttc tcaggttcgc ttcttcttct tcgtactcat ggttgacgta gatataggac
     9661 gccttcgcat tgaccgttcg ggaggcgatc agacagccct caatcaactg atgcggaata
     9721 tgtttgagaa ggtggcggtc cttgaaagtt cccggctcat gctcaccggc attgcacaca
     9781 aaatagcgct ccggcacgcg atggttaaga accttgtccc acttgatacc ggttggaaat
     9841 cctgctccgc cacgcccgcg cagaccggcc ttcttgattt cgccaacgac atcagccggc
     9901 gtgagttcct tcaggcactt tcgccatgcg tcgtagccgc cggtcttgag gtaggggtcg
     9961 atttcccatg gcgccccatc aagtttctgc aatacccgtg gttgaggtag ggtcacgttg
    10021 ttccctttcg ggggttacac ccccaggtta gcaggtagct actatacggc tgcgcttcga
    10081 gagccgtcaa gccacgttcg cctgattagg cctcaatctc aatcagttag gcctgataga
    10141 cctacgacaa ctcagcccaa agactcgctc cgaggggctg ataactcaca ccacaggcga
    10201 gcgcatgaaa aaggggactg atcacccact cggatgacca atcccccttc attctgtcca
    10261 cgcgtaacgt tacttcaaat ggctccctgc gcccatgaag aacaacatcg ggatggacaa
    10321 caggaaattg atccgcgaag catagagcgc ggttttaccc cactgagcca tctccggcgg
    10381 agccggcgtc ccctgtgcgg cagcggccac cgcagcaatg accttcttct gattcggcca
    10441 aatgataccg tggacatttc ccatcatgat gatgccgagg agcccaccga tgccgagtgc
    10501 caacgcccca gtgcccccct tggaatagag gtagccatag agcacgatgc ccgcaagcac
    10561 ggtcaccgtg gcaccatgac ggaaccagtt cagcgcggca ggcatcagct ttggaatgac
    10621 gatgttcttc gtcggcccgt ccaagctctt gagaaagccc gcattgatca ggttaaagaa
    10681 atacaacagt ccgatccagg tgatgcccgc caaaaagtgg acccagcgga caaccagacc
    10741 gaaccaatcg gcctcgcccg ctcccactcc ggccatacct agatagacga ctatcaatcc
    10801 gacagcaagc gcaactcctg cgcccaatgt ctgatacgga tcctccagaa atttcatacg
    10861 ccccccaact gtaacggttt actgccaacc acgttctcta ctgcagggtg ccgagccttg
    10921 tcaagcaacc tgctatcctc gctgtcttgg tgggcactac ctacgccgtc aacagacgat
    10981 caaccacgcc gcacaggtct cgcaccgcat cggatgaagc ctgtagcgcc gtccgttcct
    11041 cgactgtcag atcgtattcc acaatggatt ccccaccccc gcggccgagc ctgaccggca
    11101 cgccgacgat gacatccttt aatccgtatt caccttcgca gagcatcgcg cagggaacca
    11161 cacgcttctg atccttcatg atcgcctcaa ccatatcaac tactgaggct gacggcgcat
    11221 aaaatgcgct gccggttttc agtaatccca cgatttcggc tccgccgtcc tgcgtgcgct
    11281 tgatcaaggc cgcgagacgc tcagatgaca atcgatccgt gataggttta cctgccacgg
    11341 tcgtttggcg aacgagcggc accatggtat ctccgtgacc acccaacacc atcgcctgca
    11401 catccgtccc cggcacatca agctcctccg cgacaaacga ccgtagccgt gcggtgtcca
    11461 atacgcccgc catgccgagg acgcgcgact tgggcaggcc gctgactttt cgcgcgacat
    11521 gcaccatggc atccagagga ttcgtcacca ggatcaaaat gatgttcggt gaacgtgaca
    11581 ccaattcctg caccacagtc ttgacgattt tggcattggt gcccaggagt tcatcccggc
    11641 tcatgcccgg ctttctggga atccccgacg taatcaccgc gatggatgac ccggccgtct
    11701 catcatatcc gtttgtcccg accacgcggg tactgtatcc gcagacggga ccggcctgag
    11761 tgatatccag tgcttttcct tgtggaattc ccggcacgat gtcgaccaac acgacatcat
    11821 aggcattctt ctctgccaac cgctgcgcgg ccgttccacc gacattcccc gcgcccacca
    11881 ctgtgatttt tggtcgtccc attatttcca cctcgtgaag cgtgaacggt gaggtctcgg
    11941 gtgcaaagtc accttcgcac ctgcttcaca catttcacgc cggttcgtgc cccgtccagc
    12001 cgccgacctt gaaattgatc gtgcagacaa aattatcgcc gtcatagaaa ttcactttaa
    12061 ttttcttttt cccgtacttc gcggcaagaa attcttccgg cgcatccacc aggtcctgat
    12121 acccgcccgg cggatattca cccagctcgt gaaacaccac gatcatgccg gctttcactt
    12181 cctgcaaaat tttggcggtg caccgcggat agatttccca aaacttggca tcaatcgctt
    12241 ctcgcgtagg ctccggacgc tcttctccgg gcttgaagtc ccgattcgca aacttggcct
    12301 gccgtctcac atagggatat tgatggcccg tgaacaattt atacagccag ggcggcacgg
    12361 tcggtctggg agactgctca atcatgattc gtatgctcca atgctgaaaa ggccctatgt
    12421 gcaaccggtc aggccgtcag ccgatggtaa atccttggaa gcttctccgg caacccctcc
    12481 acccgatcaa tcaccacata gcgcacatcc ccgtacatcc gtcgaagata gggatccgcc
    12541 tgtttgtcga tcgtgataca gaccggttcg atcccgctcg cccgggcttc ccgcagcgcc
    12601 atgcgtgtat cttccaagga atactcgtcc ttgtatccgt catccaacgg acggccgtcg
    12661 ctgatcatga ccaacaagcg agtttttgcc tgacgagcga ggagcttggt cgtcgcatgc
    12721 cgaatggccg caccatcgcg attctgctgc aagggaacca tgccccccag cttcgcgccc
    12781 gcccgcccag tcactagttg gtcaaaatct ttgactacga caaaatcaac cagatggcgg
    12841 ccctgcccgg agtagccgta gacggcaaat tggtccccga tcgcagacag cgcttcacac
    12901 aagaggacca atccctgctt ttcgatatca atgattcgtc gtccccgttc aacctggcgg
    12961 ctggtggacc cgctgagatc gaccaaaaac gccgcggcca cctcacgttc ccgcttttcg
    13021 cgccgcacat agatccgttc cgatggctca atgcccgctt gtatatcggc gagtcggccg
    13081 atgacggcat ccatatccag gtcttcacca tccacttggc ctggaacacg ccgcaatccc
    13141 gggggacgca ggctttcgaa atagcggcgc aggagcgcga ccggcccccg ctgggctgcc
    13201 aaggtccctt ccacaaaatc cgacgaccct tcgaccgcct cccgttcgac cacgcgacac
    13261 caattcatcc gatagtcctg aatggcggca tcccattccc gatagcggac cgccttgacc
    13321 gtctcagtct gatcatcttg tggccgcgcg ccctcacctt ccaccgccaa catctcttca
    13381 accagcgagg gctgtcgacg acccggcacc aacatctctt gagacatctg ctgcgaacgc
    13441 gccgtaccgg caccagacga tccgcctgaa tcaccggcga gtccagctcc gccttcacta
    13501 tccccctggc cggacgcagg actctgttca ggtgtcgatt ccgtgcggtc tcgaaccaga
    13561 ttcgggtcca tcgcgccacg atagtcccag ttgtccattg gacgatacgt gtcggaaaga
    13621 tcctccgagg ctttcggcgc aggaattgat tgttcaggcg attccgcttc atctggactg
    13681 ggaatagcct cgcgacggat ggccaataac tcatccatcg agacatagag acggtcggcc
    13741 aatcgcacgg cctcctccgc agtggcctgc ggatggaaga tggtttgaca aaggctccag
    13801 agatgcatga tctcgcgctt gagcgcatca ggaagcggca tggaaccggc ctcgaccgtg
    13861 gaaaggagca ggagctgatc gacgaccaat tcgcgcaccg tcatgccatg cgccagactt
    13921 cgaacctgca ccgcatcctt tgcgatcacg gctaaatccc ggctcaggcc cggatattcc
    13981 cgacgcagca aatattcgac tcgggcatct tcgacgagcg cccacagatc gcggatgagc
    14041 cctggctgag gataccgtgc aaacatatcg cctaaagtac ggacttcccg taaggccggg
    14101 cgtccataac gttgcgtcag ttctttactc acatcttcca gtcgggcgag gggcagatcg
    14161 aaggtgccga attccaaatg ccctgcctca tgcgcggcca tgaccatata gagacggaca
    14221 ttctcctcat aggtggagtg ccgcctgacg agcgacggca acccgatgct ccgcccgtcg
    14281 tcgctgaccg tggcacgtgg catcggttgc gctgattcca gcgagtcggg cagatcgtaa
    14341 atccgtagat cgcggccgca gagggcctgg gcgaataact tgacccgcct ggcgatctgc
    14401 cgcaacggca ccccgctcat ggcctgcgat aacgcgccca gtgctttttc ggactccatg
    14461 ccaaagtagg cgcggccccc ttccacgctg tactccagca cgtccatccc ggaagcaaac
    14521 caggactgaa acttctgttg cgccccttca cctgatccaa tcaatgcaac caattcaggg
    14581 caacggcgca tataggccaa ggctgtctcg gcatcgcgtt ccgccaggat cagaccgtat
    14641 tgcagcacct gcctccgcca ttcatcagtc gggagaccgc ggagaatcga cggggcttcc
    14701 gacagaaggg caatacccat ctccggcgac cgctctgcta tctgcgcgcc cacgcgaata
    14761 acttggtggc gtgatatctg atcgggaata tccgcaagga tgccgggact cgtccggaag
    14821 aattccagcg ctcccagata gtccggcttg ccgaggctat tgtcgacaat gagtgtcatc
    14881 ccgaactgca tccaggctcg tacggtatcc agagagagca cgcgggcgat ggcaggaatc
    14941 tgacggatat actccacggc caaggcaaaa tcggtctctg acaactcaca ggccagttcc
    15001 atccacgtcg gccaatcggc tgggggcaga acggggacaa cttccggcgc aactcgaata
    15061 aattccaccg ccacattggc ctggcggtca gccaactcca aaccggcagc caataccacg
    15121 gctcgctgag gcggatcgag tagaccgagc aggaggggac tctccttgaa aaatcgtaat
    15181 gccaacgctc ccgaatcagc cgcgatggct acgcccaggt cgagccatgg cagcacctcg
    15241 gccagcaaac cccggcgttg cagttccaac agagcctcga tcgcacccct ggccgctttc
    15301 ggcgcagcgt ctgtgagctc ctccagcaac agccacaccc ccgccggcgc atccggtctg
    15361 gtggccgagg caacgatggc gtcaaccatt gtcgtagcgg aagccggccc gagttggtcg
    15421 gccaatcgct caataagttg tgggaaggag tgcccgggtg ccatgtactg tgatccattt
    15481 tgagggaagt cgtggattgt aaaggacaca atttcgcttg taaactgtgt cgtcgcaggc
    15541 gaccgcctgg taccgatcga ctgtttcact gaaccaatgt cacgcggggg acggaatggc
    15601 tgagccgagc aaagaaagtc tcgagaagat gtggaagtat gtgaaaggct tcgcggagaa
    15661 gagcggcacg gccatgcacc cgaatcctgc cgtgaccaac gccgtggtgt tggggttagc
    15721 cgcccacgtc gatgagttgg gaaagcccct ctgcccctgc aatttctacc ccgataaaca
    15781 agccgaggcc aaactgcgtc gctggatgtg tgcgtgcgac gaaatgcaaa tttacaaata
    15841 ttgccattgc ctgctcttcg tcaatgaaga agggctgccg attacggagt acttgcccga
    15901 agaccacgag ggacgccaat gttacggcct cgtccctgat ccgactccgg gcaaaggccg
    15961 agcacttcgc cacaaagcgc tgcccatggc tccgaaggtg ccggaatccc cggctacgcc
    16021 ggattcctcc gcacaatcgt gatcagccgg ttttgctgca atccgatagt cggccttcgc
    16081 tatggcttgg gagttgtcgc cgtattgacc tctttgagta tggccggctg cctccacaca
    16141 atggaggctc ttgatccgag agcgttgccc gtcacgacag ctccggcgca acgattgcag
    16201 tcatacgtct ccttcctcgc gagtcccgca ctggctggtc gccaaccggg aacgccaggc
    16261 aaccgccagg cggcccaata catcgaagaa caatttcgtc tcgtcggact gcagccgctt
    16321 gcctcgcttg aaggctatcg gcagaccatt tcggatcgca tgggcgacaa cgtcatcggg
    16381 gtgatcccgc cgatcgatca cacagctcgc acccgctgga ttgtgatcgg ggcccattac
    16441 gatcatctgg ggtatccctt tcttggagcc gacgataacg cctcctcgat cgccatcctg
    16501 attgagacag ccagaaatct gacgggactt tcacgccacg gcatcatgtt tgtcgcgttc
    16561 aacggtgagg aacttcccta cttcggaacg gccgaaatgg gatcacagtt tttgtttcac
    16621 cacctgccgc cggaagtcgg cgctgcggaa aatcttcaag ccgcgatcat catggatttg
    16681 atgggaggcg tgcattgggc tccgctgaag gacgcgattt ttgccacggg agcagagaaa
    16741 agccctgcgt tgtatcgacg ggtcactgaa acccgggtct cacccctcac gatctaccgt
    16801 ggaggaattc acctggtcga agaaattccc gaccatggtc acaaggcctt cagcgactac
    16861 gatgtattcc gcaaccagaa ggtcccattc ctgtttctct ccgctgcgcg atcaccccgt
    16921 tatcacacgg caggcgacgt cgccagcacg ctccactacg aacgcatggc cgcgacggtg
    16981 gaatggctac gccaactgct cgcgctcatg tcgcaggacg aggcgcccta tgcttttgat
    17041 gcccaccgcg tcgaatttgc cgatgaagtc gcctcgttca ccagccttgt ggagcaggcg
    17101 cttcacgagg agacgcgcat tccaggcaca tcacgctggt cgttgagaaa attggagcag
    17161 gatcttgaat ggctgcggga ggtacgcggt tccgccccga cgccgcaaca attggagcgg
    17221 ctggaacgga tttctattcg tgttcaatgc ctgatggccg actattcagg ctgctttaca
    17281 ttttgacgac tgaacctgca cggcctttga tcgaaaaccg atcattattg ccacccctcg
    17341 gtctcgtagc ccttttcctt cagacaacgt tcgacgaatg ccgcataatc ttgttgcggg
    17401 tgtaacggct tgtaggttcc ggcgaacagc cccaagattc caccgacggc gccgcccgct
    17461 gccgcaccga tggcaatccc agttggcccc ccgaccagtc ctgccgaggc ccctactgcc
    17521 gccccaccga tgacgcccag cgcggcgccg gcgccggcat tcccgctgcg attcgttcca
    17581 tgctgcaatc cggcccgctc ggccttcacc gcacagaccg cggcttcatc ctgggcttgt
    17641 tgctgcccgt ggagttgcaa ataggtagtc gacttgaaga tgggttcagg ccctgcacat
    17701 ccgctcacga ccacgacaat cagcgacagt gaccatcgaa aaatgatgga cctgattttt
    17761 ctcagaacag acgaccgcgg ccatagctcg acacccggct caccgtgcat aacatcctcg
    17821 catcaagagt gcctcatgca ttcagaggtg cgacccaacc gcggcttact caagttgaag
    17881 ttgtgcgcgc acctgttcgg cagaacgtcc cctcagtaaa attcgcttct ggcggctact
    17941 cgtcccgctg agaatctcga cgccgctcac gggcaggccg catgtgcggg caaggaaccg
    18001 gcacaactct tcgttggctg caccatcagc aggcggtgca gccacacgaa ttttgactgc
    18061 atcaccgtga agccctgcac attgagtccg ggaagctttc ggctggacat gaacggaaat
    18121 aacggcgccc tcgggtgtgg tgcgaacgac ctgcgctgct gccgaggatt tggactcccg
    18181 gccggaattg tgggtggtca caacattgtg aagcacgcag agtcggtgag gtgcaaaaac
    18241 gagcggctag aaaatcgctt tcacgacctc gatgatgctg cgcaacatgt ccggatcatc
    18301 ggtaatcggc ctggcaatgg cgacatcgca cgcgcgatcc gcgggcacgc cctgcttgat
    18361 cagggtcgcc gcatagatca acaatcgggt gctgacgcct tcttcaagcc cgtgattttt
    18421 gaggttacga accttctccg caatcttgac cagtcgctgc gcaatctcgg gactgactcc
    18481 ggcttcatgc gccacgaccc ggcactcgat atccggcagc ggataatcga actcgatggc
    18541 catgaaccgc tgcttggtgc tttgcttcag atctttcaga atactttgat aaccggggtt
    18601 ataggagatc accagcatga actcatccac cgcctcgatg acctgtcctt ttttttcaat
    18661 gggcagcagg cgccggtcgt cgctcagagg atggataatg accgtggtgt ctttgcgagc
    18721 ctcgacgatt tcatccagat agacaatcgc tccctgcttt acacccaggg tcagcggacc
    18781 atccatccac actgtctcct gtcccttcaa gagatagcgg ccgacaaggt cggaagcggt
    18841 caaatcctcg tggcacgcga cagtgatgag cggacgcccc agccgatagg ccatgtgctg
    18901 gacgaaccgc gtttttccgc agcccgtggg gcccttgagc atcaccggca tgcggctttg
    18961 cgccgcaatg gtaaagagcc cgacctcgcc ccgcacgtcg gcgtaaaacg gctcgcgcgc
    19021 gatccggtaa tggtcgatgt cttgtgcctg tccttttggc gccgtcgctg ctcgcccctc
    19081 gctcattccg ccactcctgc ctgaaattaa ataaagtgca tggtgcgcca cgataaacgg
    19141 aaaccggcag gaagttcaag cgaccgacag aaacctcggg tcaactgacc ttcgacccat
    19201 ccaacacttc acgaactttt tgggccagcg tcgtgggcgt gaagggtttc tgcagaaagg
    19261 aatgctcggt atcaatgcca cccttagaga aggccgggtg atccggataa ccggacatat
    19321 aaagcacttt gacttccgga cggaccgtcg caagtttttc ggcgacttcc gggccgctca
    19381 tttgtggcat gactacatcg gtgaggagca aatggatggg accggcatgt ttggcacccg
    19441 tcagcaatgc ttcgatgcca tgcctggctt ccagcactgt atatccatgg agccgcaacg
    19501 tttcattgac caggcgtcgc acgcccggtt catcctcgac caacaacaca gtctcgcgcc
    19561 ctctgaccga atcaccgccc acggcaactg attccgtttc ctcactgacc gcttcaacac
    19621 gcgggaaaaa tattttaaag gtggccccac ggcccggctt gctctcgacc tcaatgaacc
    19681 ccccgctttg tttcacaatc ccatagaccg tagacaatcc cagtcccgtc cccttgccct
    19741 tttccttggt agtaaaaaat ggttcaaaga cgtgggactg cgtatcttcg tccatcccgt
    19801 ggccggtatc acgcacgatg agcgcgatgt acgaaccagg ctcggcgccc atcggcgggc
    19861 ggttgcggcg ctggccgaat tgcacgttgg ccgtctcgac ggtcagccgg ccgccgtccg
    19921 gcatggcatc gcgggcgttc acggccagat tcatgatgac ctgttcgacc tgcccgggat
    19981 ccgccttcag ttgtcccaat cccggatcca gcacagtaca gagttcgacg atgtcttcgc
    20041 ccaataagcg ccggagcatg ccttccatat tatggatcac cgagttcagg tccagcacct
    20101 tcggagcgat aaattgccgg cgactgaagg ccaacaattg gccggtcaga ccggatgcac
    20161 gatccgccgc ctttttgacc tcttccaatt ccttccggaa tccgtcggtc ggggaaagac
    20221 gactcagcac cagttcgctg tagcctcgaa tcactgtcag catgttattg aaatcgtgcg
    20281 cgacgcctcc ggcgaggcgt ccgaccgctt ccattttttg cgactgctgc agctgcgctt
    20341 ccgtgcgtcg cagggcatcc tcggcgcggg ctcgttcgcc aaactgcccg atcttgagac
    20401 cgacgtcggc gaccatcttc aagagttgct cgtccggctg tttgatttcg cgccggaaga
    20461 gctcgatgac gccttctact tcggttccga ctcgaacggg gaatccgaag gcgccgtgta
    20521 atcccacgcg cgaagctgca ccgccccgag ggaaattcgc ttcctgcacg acgtccttga
    20581 tccacgtcgg ctcccccgcc tgccaaatcc gtccgggaag tcccttgccg agcgcaaacg
    20641 tatgccgcca ggtgtcgaga acaaattctt cggcattgag tcccggtgac tgccacacat
    20701 ccaaacaacg cagcacactt ccctgccggt cgagccgcca gaatatgccc aattcccatt
    20761 ccaggctttc gcagatggcc tggagaattt ttggaccagc ttcttccaaa gttcgcgatt
    20821 ccgccagcac gcgcgtgacg gcgtactggg aagccagttg gcgttccgtc cgtttgcgcg
    20881 cggtgatatc gcgaatgaac gcgctgaaaa tatacgcgtc gcccagcctg gccggagaga
    20941 tggtaatttc caccgggaat tcatgcccgt cgcgatgact ccccatcatc tcaatgcgac
    21001 gattgagaag agcgccttgt ccggtttcga ggaaatgccg caatcctcgc tcatgcccat
    21061 cccggtcccg gacgggaatc accgtctccg atacgcgacg gccgagcgct tcctcgcgtt
    21121 cccagccgaa gatgttgacg gcctgcgcat tccaatccgt gatgatgccg cccgcatcca
    21181 tggaaatgac ggcgtctaac gccgtatcga cgatgacccg gttgcgttcc tggctctgca
    21241 agagggccgc ctccgccgcg cgggcccatt cgctctcttg ctgtgcctgc cgatgctgct
    21301 cacgcaatcg tccggtggcc cacaacagca atcccaccat cggaagccat acgaccactc
    21361 cggcaatgcc gagtttccac atcatcgccc ggatcggcag cagaatgacg tctcgatcga
    21421 tccgcaagag aatggtccac tgcaagctgg aataatcgct gtatcccttc gtctgggaat
    21481 agccggtcac gacggggaca tgacggcgaa cgtgtatttc ttcaacatat cccgccttgc
    21541 cggaactgct ccgcaagact gagggcagat tgagttcacg caaattgacg cggtcgatgt
    21601 tggaggagga ggaatccaca aaaaccagac cgcccttggt caagaattgg tattcgaagg
    21661 gaccggccgc gccatcggtc tgttgaattt cgcgtaccgt ttcgatgaga acatcttcca
    21721 gcatcggcag gccgatccgg gtggtgactg cgccgagaaa ctgcccgctg acactgagaa
    21781 tgggcgcggt aaacgcgacc gattcgatct tatgatttga ttcatggatc tcgacttcat
    21841 cgacctgcac cgtgtgggtc tgccggaccc ggtcgaacca gccgctccgg ccgtaatcct
    21901 ggccgatgat ggatgaatcc gttgccgcaa tcatccgccc gtgaggatcc gtcaccccca
    21961 gccagagata gacgggcgca taggtctctt tcatccactg cagatattcg ttgatatatt
    22021 ttttatcggc agagcggtcg gtgaacgccc gcgccatcat gcggacatcg gcatgacgtt
    22081 cgaagagcag ccggtcgagt ttgtccgcaa tctccgccga cgcgagcgtg agattttctc
    22141 cggtggcttc aaccatccgc acctcgatcg agcgcaacat gaccacgccg atcaccaacg
    22201 caacaatcgt cacacccaca atcagcacgg gaagccacgg ataggatcgg cgagggaaac
    22261 ctggaagggg cgaggccgtc atcgtcggaa ctccgtcaca acataattcg gcaacaatcg
    22321 ggcaggatga taccagcgct tggctttcgc cagactcgga aggccggaat catccatcgt
    22381 attgatccaa catgcgcctt tagcgtgcgc ttggcgacag aattcacgaa acacgaacgc
    22441 gcccgccccc acaatatccc gatccgccac ttccagcaag acacagaaca ctgattgatt
    22501 gagccacagc cccatcgtgt aggcccgaat gcgccccccg actcgcacca ctgctccggt
    22561 cagccccagc tcactatgtg ccgacaaggc tgtctcatgg gctccgacgg catcctccag
    22621 cagagcattg gcccactcat ccgatccgga ccgcagcttc tgttctctcc attcacgaaa
    22681 cagactcacg caagccgtct ggtcgcgaca atcatagggc tcaagaaccc cgccatgttc
    22741 gcgcacgaac cggttgcagg ctgcgcgagg cgatcggtac gcttctcctg caaggtcgac
    22801 cagatctgcg gcagcgtaca gatagtctgg ctctttttca cccgtctcat aacccgacgc
    22861 acgcaatgcg ggcacgaacg attccggcac attctccatc cttgtgacgg acgaatcccc
    22921 attccacgcc cgcatcagcc gaaaggcctc ggccgtgggc tgatcgatcg gcccactccc
    22981 cagcggaggc aaaaccagga atatcccgtc gggcgactcg gcgaagaggc acagatgccc
    23041 ctgcagctct gcccatgtat acgtcagccg atgtttccag atgtaatgat aggcgaacga
    23101 agcagcggcg agcgggctct cctgaaaacc ggcggtgacg gtcagtgcgg cttctattcg
    23161 acctcgatcc tgaatggtca gggcctgccg acgtatctgc ggctttgctc gctgcacacg
    23221 ttcggtcacc cgtgccaagg tgcgcaaagg gataacatcg tcctgatacc gtccgatgag
    23281 cctggggtat cgcgcaagcg tgtccacccg ctcatccgct tcaacccatt gcgccacccc
    23341 gtcgactgcc tggtcaacca gcgacgaggg agcgtcgcgc atatagggac acttcgtatc
    23401 ccacccgagg aggacgtggg tgcgctcggc atcccacatc aacgcaaacg gatagagccg
    23461 gcagtccagg gggcggacct catagatccg gcagtgcgag gtctcactgt caaaggccgg
    23521 gcagacatat ccttcgccag ccgggttcgg gaccagatcg atttgcgtgc cttcggtagt
    23581 cgggaacagt tccggtgtca ggccggccgc cactgctgca cggatctcat cgccggtgaa
    23641 gaagggacgc aagaagctgt ctacctcggg aaaccggcaa cagacgtcgc aatgaagaca
    23701 ggtcgtactg ggcacgatct gcaggagaga ctgccctacg ggccgtgtgg gattcgctgg
    23761 ggagactgga acgagtggac tcgtcatgag ctggcgcgca ttgtatcact aacctgcacg
    23821 tggtcctact gttccacatg tttgccgtcc aagggctgtg gagcagatcc accagctgcc
    23881 gacgagcctc tcaggcgcct cgccgacttg taccagtgca agaggccatg gtacgatttg
    23941 ctccacctcg aatccgaccg cgactgcgaa gcatgcgact gcgctgcgag catcgaggat
    24001 attgcattgg cactggcaca acattcacgt gagccgttga actccgacga agcccgccga
    24061 cgttgtctcg caatccgtga tgcgctcgtt cacgccggcg tctatggccc ggccgacacg
    24121 gaacagggca cagccacggc cggttccgga gtttcgccgg tacgattccg cctttctccg
    24181 acaccctttc cgctttcctc gtcggaagtc atgtgttttc agcacttagg gcaggatctt
    24241 ctggcgtttt accgggcatt aaaccgcctc tatcaagaaa gtgcccgcgg aatccagccc
    24301 gggtgggttg cggcctatct cgatcagggt aaaccggaat ccctcgtcac gttcagccgg
    24361 atgaaccggt tccgtaacct catccccggc atcattcggc cggatgtcat cccgacggcc
    24421 gatggcatgg tgatcacgga gctcgactcc gtgcccggcg gaataggcct gacggcgtgc
    24481 atgacacgcg cctatgatga tcagctcggc ctcagccctt cgcctgcatc tccaccatcc
    24541 gaacggtctc cgctgattgc cggtcgtgac ggcatgttgc aggggtttgc cgctatgctg
    24601 cgcgctcagc agggcgaccg ggaagggtgc ctggcgatcg ttgtctccga cgaggcgaaa
    24661 gaataccgcc cggagatgga atggatggcc cagcgcctgc gcgatatcgg tttccaggcg
    24721 ttttgtgtcg aaccgcgagc ggttcgttgt accgatgaag ggctgtatct ccaggatgag
    24781 cacggcatgc ggaccatcgg attgctctac cgcttttttg agctcttcga cttgaagaac
    24841 atcccgaaat ccgagctcat gatgtatgct gccaagaagg gacaggtcgc cgtcacgccg
    24901 ccgtacaagc cggccttgga ggaaaagctc gccttcgggc tattccatca ccctgaactg
    24961 gagccattct ggaaggagac gttgaccgcc gagacgagaa cgaacttggg acaattattc
    25021 cctcgcacct ggattctcga cccgcaaccc gtacctccga cagccgtcat cccacatctc
    25081 ctcatcagcg ggcgggccgt atcggatttt cgccgcctgg cccacacgac tcaaaaagaa
    25141 cggcagctgg tggtcaagcc ctcaggattc tccgaactcg cctggggcag ccgtggcgtc
    25201 tccatcgggc atgacctgcc gcaagtcgaa tgggctgcgg ccttggagca ggcgcttcag
    25261 tccttttcca cgacgcctta cgtgcttcaa gaattccata aaggccgttt gttcgaagcg
    25321 tcctactggg atgaacgaag cgacaccgtg gtgccgatgt ccgggcgggt tcgcttatcc
    25381 ccctattatt ttgttgccgg cgacaaggcc gagctggggg gaatcctggc aacactctgt
    25441 cctgccaata agaagatcat tcatggcatg gtcgacgcaa tcatggcgcc ctgtgcgatc
    25501 gcgccaggcg tggctgccta gggtgaggtg aggacgggaa tacaatctgt tcagtggacg
    25561 gctattcact cttttcccca ataggaactc atggctctga ccttctatca tgttcgatgg
    25621 tgtcccgatt gcgcggtcgt tcgggatcgt ctcagtgaat tgaacctgtc ctacgaagac
    25681 gtggtcgtgc cggacttccg gccaatgcgc aaacaagtct acgaagtatc cggccagtac
    25741 tacgtccccg tcttgaaaga cggagacacg gtgctcacag aaacccacga cattctggca
    25801 cacctgcaga cacactacga aaaggcgcaa ccgtgaggag gtcgctatta gggcgaccgt
    25861 tgaggatcac ggatcagacg agatgcccgg acaaggctcc ggccaatacg gcccggccgg
    25921 ttggaacggc atcagcgaaa ggatagaaac gtggaggatt ggaaacgcat cctggctcaa
    25981 agcgtggtga agccaaaaga cctggccgag cggctcggtg tcgaccccaa agaagtcgaa
    26041 gacatcgtgg gcgactatcc catgcgcatt acgccgaccg tgttggccac catcaaggag
    26101 aagggggacg cgatctggaa gcaggtcgtg ccggatcgcg ctgaaatggc cgacgccgat
    26161 gcggaggatg atccgctcga agaagacctg atgagcccgg tgccccatct ggtccaccgg
    26221 tatccagacc gtgtgctgtt gatggtcacc aatcagtgtc caatttactg ccggttttgt
    26281 acccgaaaac ggctggtcgg caaaccgggc ttcctcaaaa agggggagct cgacagggcc
    26341 atcgcgtatc tgcgggaaca tcaggaggta cgggatgtca tcctgtccgg tggtgatcca
    26401 ttgctactgc cggatcacct cttggaacga atcctcaaat cgctccgcac gattccgcat
    26461 ctggaattga tccgcatcgg gacgcgtgta ccgggcagcc tgcctgagcg aattaccccg
    26521 aaactctgcg agatcatcaa gaaatatcat cccttctata tgaacctgca cttcaaccat
    26581 ccggatgaac tgacgccgga cgtgaagcgt gcgtgcggca tgctggcgga cgccggggtt
    26641 cccctgggag cacagactgt gttactgaag ggggttaacg atgaccccga ggtcatgaaa
    26701 cggctcatgc accagctact gctcgcgcgc gtcaaaccct attacctgta tcaagcggat
    26761 ttgaccaaag gcacgaatca tttccggacc tcggtggaaa cagggctcaa gatcatcaag
    26821 tcgttgcaag gtcataccag cgggatggca gtgcctcact ttgtgatcga tgcccccggc
    26881 ggcggcggga aaattccgct actcccggga gactatctgg tcaatctgga tgaagacggc
    26941 gcggtgttga gaaactacga aaacaatacg tatcactatc ctcagcccgg ctcccctcaa
    27001 ggccgcgagt tgccgatggt tggcgcccac ccttcctggg ccgcatccgg caacgggtgt
    27061 gacggagggg gtgagcacct gtgaaacggg ccgcgtcctc gaaggggatt ctaccttacc
    27121 agcacattac ccaactgatc acccgtggcg ccatcaagtc ggacgccccg atcgaagacc
    27181 ggcagatcca gccggccagt ctggacttgc ggctgggcaa aaaagcctat cggctgatca
    27241 gcagcttctt gcccgagtta tcggccatca gcagccgtct caatgtgctc gacttttatc
    27301 agtctgatct cgtcatgtat gagatggatc tgacgcaagg cgccattctg gagaagggcc
    27361 atgtctatct ggtgccgctc ctggagcacc tcgaactgcc ggccacgctc cgcgcccgtg
    27421 ccaaccccaa aagcacgaca gggcgcctgg acgtgttcac ccgtgtcgta accgacttga
    27481 acgccgggtt tgatgagatc cgggccggct acacagggcc cctgtatctg gaagtggttc
    27541 cccggtcatt tgccattaag gtgcgcaccg gtgattgcct caatcaaatc cggttcgtac
    27601 gcggagatgc aacggtttcg gataccgctc tccaaaaatt gcatgcggcc gaccccctgc
    27661 tcttccgcaa tgcgtcctcg ccgaaaccgc tccctcagaa agagttccgg acggagcgcg
    27721 gactgttttt gcggatcgac ctcaccggga gcgctcacga ggaggccgtc atcggctatc
    27781 gggcaaagaa gaacagtcat gtcattgatc tggcgaagat cggtcactat gcggccttgg
    27841 atttctggga accgctcaaa aggcaccggc acgacagcct gctattggag ccggaagagt
    27901 tttatatcct ggcctccaag gaacgaatcc gcgtcccgtc cgggtatgcc gccgaaatgg
    27961 tcgcgtacga agcggcctgc ggtgagctac gcacgcatta tgcgggattc ttcgaccccg
    28021 gtttcgggta tggcgatggg acgatgcgcg gcacgcaggt cgtgctggaa gttcgcccgc
    28081 acgatgtgcc attcctgatt catgacgggc aaaccttctt caaagtagtg tatgatcgaa
    28141 tgctgacggt cccatcacaa gtgtatggga cagcgctcgg ctcgtcatac caacgacaag
    28201 ccctcaccct cagcaaacat tttaaggcct gaacatgcca cctcccagcg acctcccaga
    28261 tccctctacc cgtccgtcct ggccgcctcc acatcgcccg gacgatgaaa gcacctccga
    28321 acggcaggcg cacgtcttcg ggatgatgat cggcacggcg ttgatgttca tcggtttttt
    28381 ggacgtgttt ctctccatca gcggtggatt tgaaattaac gtcgtcccgc tcttgattta
    28441 cttcagcggc atcgccgtct gggctaacgc ggtcgtggaa aatccgaccg tccgctattc
    28501 cttgatggcc ggatccgtcc tgatcagtct ggccttcttt cattacggcg aggtcctgtt
    28561 ctggcataag caggtggtct tctggtccac cgtcgccatc gtgatgtact tcatgttcaa
    28621 ggaccccaaa ccgaccgcgt aaatggccgc gagcggccct catacgacgg gtgctctccg
    28681 gctccgatga ccatcgccgt catcatgccg gtgctgaacg aatcccgttg cctcgctcac
    28741 atcctcgccc atactcaggc attagggttt gacgaggtga tcatcgtcga cggcgggagt
    28801 tccgacgaca ccttctccat tgcgcagtca ttcgccaagc aatgtgatac agatccctta
    28861 cgcgcgagcg gatcgatgcg tgtattgact gccccacgcg ggcgtgcgag gcagctcaat
    28921 accggcgcgg ctgcgagcgc gtgtgatgtc ctgctctttt tgcatgctga caccctcctc
    28981 ccatcgaacg cgcggcgggc cgtcttgacc gcactctcag atgcagccgc cgtcggcggc
    29041 cgcttcaatg tccggtttga ctcctctcga ctgacagccc tggtcgtcag cagtctgatg
    29101 aacgtgcgct cgcgctggag cggaatcgcc accggcgacc aagcgatctt tgtgcggcgc
    29161 gccgtttttg agacgatggg aggattcgcc gacattcccc tgatggaaga catcgacttc
    29221 acgcggcggc tcaaacgaac cgggccgctg gtctccttgt cggatgaggt cgtcaccgcc
    29281 ttccgtcgat gggaacaaca gggtcctctt cgcacaattc tgttgatgtg gacccttcgc
    29341 ttcctgtact ggatcggggt caacccgcat cgacttcaac atttttacag catggtgaga
    29401 tgagtgatga gccctcaacc ctcggaccgt aaacagcctg cgtccgccgc cctggtgatc
    29461 ttcgccaaag cgccggtccc gggacaggtg aaaaccagac tttgtccggc cttgaccgaa
    29521 gacgaagccg ccacgcttca cggcagcttt gtcctcgaca cgctcgaacg caccaaggcc
    29581 gcggccagca ccttcaagct gtcgttcgat cgtattctgg cctgcgcgcc ctcgagcacg
    29641 cacgtgtttt tccgcatcat ggaagcacgc tacggggtga cactcttcga tcaggaaggc
    29701 gacgacctcg gcgctcgcat gcaacacacg ttcgctcagc tgttcgcccg cgggtaccga
    29761 cacatctgcc tggtgggaac tgatgtcccc tcgctgccgc ttacacacta tcgcgatgcc
    29821 tttgccgcac tgacccggca tgatctcgtc ctgggaccgg ccaacgacgg cgggtactac
    29881 ctgatcggca tgaccaggcc gcacccgaaa ctgtttgcgc atattccctg gtcgacagat
    29941 caggtcctgg cactcactca gcagaacgct gccgcagaag gcctgaacac cgcacttctg
    30001 ccctcctgga acgatgtgga cacactggag gatctccaag ccttgatcga ggactgcgtc
    30061 gcggataaga aacgtccgaa gaaggaacgc atctattcaa acagaacggc gggaacactg
    30121 gagttgctcg ccaagcgcct gcgcgaacgg cggggctaga aggagacggt atgccgaacc
    30181 aggttgcact catcacgggc ggagccaagg ggatcggccg cgccatcgcc ctcgaccttg
    30241 ccgcacaaca ttggtgcatc gctatttgct accgcacgag tgccgcagcc gcagaagaga
    30301 ccagcgcggc catcatggca cgtggcggcc aagcccttgc cctgcaatgc gacgtgtccg
    30361 atccggcggc ctccaagcag ttggtcgcac aggtcgaagc gcagtgggga cggatcgacg
    30421 cgttgatcaa tggagcaggc ccttaccatc gcgtaaacct cttcgacgaa acgacaacgg
    30481 gctggcgcga gatgttcgat ggaaatcttc atccgatttt ctatctcggg caagcagtcg
    30541 cgccgggcat gaaagccagg aaacatgggc ggatcatcaa tttcagcatg gccaacgccg
    30601 accaaatggt ggcccagccg gaagtgacgg gtcattatat cgccaaagcc ggagtgttga
    30661 tcctgactcg cacactggcg aagctgctcg ccccctatgg catcacggtc aacgcgattt
    30721 cgcccggctt tatcgattca ggcagtgccc ctgcggaaga actggcagga gtggtcaaac
    30781 ggatcccagc gggatatgtg ggaagcgtca acgatacagt cgccgcggtg agatacttgc
    30841 tgagtgaaga ggcgcgctac gtcaatgggg cgaatctcca tctcagcggc ggctggggca
    30901 tctaattcac ttggcaaaaa agagaatggc cgcgatgatg ccagccatgg cataactttt
    30961 agggtccttc acggcaaaca cgacgggatc atcgtccatc cggttccgat aggcgagcat
    31021 ccaaacccgg ctgatccagt acaacatcac agggcacacc agccataaga cttccgcatg
    31081 agaatacagc agtagcacct ccttgctgct gatatagagc gccagtacca acaccgccaa
    31141 gagtccgctg gccgttccaa tactcgaaat gtgctctaga tccgtcaccc aataccctcg
    31201 cccatgcaaa cccttacctt cgacttggct catgaggcgc agctccgtga accgcttgac
    31261 cagcgccagg ctcagaaaga ggaaaagcga gaatgtcagc aaccaatggg agggggggac
    31321 accagttgca gctccgcccc cataaacacg gaccgtatag agctgagcca gaacaatcac
    31381 gtctacaagc acgaattgct tcaaataaaa cgaataccct gtagtcagag ccaggtaacc
    31441 acctagtacg agaagaaaca ccgaaggaag cgccaaggca agcaggacac cgccaatcag
    31501 acacactgca gctaaggcaa gtccaaatgg gatcgacaga ttgccagaag caaatggccg
    31561 gtttctttta cgagggtgtc ggcggtccga ctccagatcg aggcaatcat tgacgatgta
    31621 cactgcggaa gcacataaac tgaatgaaag aaaagcgagg ctcgcctgta gcaacaacca
    31681 accattcgcg agttgatggg atgccacccc tgggacaaac accagcacat tttttgacca
    31741 ctgatggact cgcaacgcct tggcgacttg cttcaccaaa ttcaccggcg cgcggaaaac
    31801 gcggctgacc gttgtcagcg ttcgagcacg actcgctaca ccggctgacg cattgacgac
    31861 gatcgcctca ctggcctctg cccacaccgg aaaatcagca gtagagtttc cggcataggc
    31921 aaaccgccga gttccatacc gttcaaccag gagcgcgacc ttacgagccc ccttcaggtt
    31981 tacggagctg tcgctgccga atacgcgtcc atcaaacaat ccgaggtgag tagccaccac
    32041 ctgtgcaaaa ctcacatggc tggctgtagc taaaatcagc tctctcccgg ccaagcgctc
    32101 tttcgagaga aactcaacca actcctggtc atagggcaac gtactagcgt caagcgtgac
    32161 cctttgagcg atctgatgct taaggtaggc tctgcctctc agtagccaaa cgggaagtag
    32221 gaaaatcgag agcggattct gcttcagaag ggccagcagt gattcccaca acaaatcggt
    32281 cttaatgagt gtgccatcaa gatcgacgca gaggggactc tttgctgaat gattctcacc
    32341 cataagcgat aaaaaggatt ctatcggctt gagggcggtc tcgcaagaaa ttccaggaac
    32401 agggtggtaa ggcgcctcgt ccggtcgcag acaagacgcc ctctttcgaa agagcaccgg
    32461 cggaatgccg gaaggaatca tgtaccaggc ttacaggacc gaaaagcctt acgattcggc
    32521 gccgaaggct tttttcaaca gcggcaccac ctcccccttc gcttccatgg ggtccaggat
    32581 atccgtgtcg ccataaaact gtccgtcgat gaagaccttc ggcaaggtcg gccaattcgt
    32641 caacttggtc aaggcctcgc gtttggcggg ctgagataag acgtcgatga gttcataggg
    32701 atacccgtac ttttcaaaaa aatgcatcgt ctcccgggta aacccgcaca tgggcatggt
    32761 tttggtgccc ttgccgtaga tcaggatttt attggcctga atttctcgct gaatttcttc
    32821 ttcaatcggg tcagccatct ggtccccctt attttttcac ttcatccggt gtcatagccg
    32881 taatctccag cgcatggatg cgcccgtctt tcatcggacc cgctaatgcc tgatagacca
    32941 tgcgatgccg gtccaggaga ttcttatcgg caaatcccgt agagatcacg cgtacaatca
    33001 aatggtcctg cgtaccggtc ttatcaatca ccgtgacagc ggcatcggga aacatttgac
    33061 ggatataggt cgttacggct tcggcggtaa tcatctgtga gcagaataac gcatccggga
    33121 agcggaaggc aatgtgaggc gctgagtcaa ttatgagatg cgtccagctg ctccacagat
    33181 ggcaatttgt cagctggccc gctacactac gcgacgatac gtagcctagg ttatgcccat
    33241 acgcagaaag gagtactcat gtctcagacg aagtctcgac agccacgccc gctcgtacaa
    33301 acgaccttgt tcgtgttggt cctggtgctc ggatgccaag ccacgctctc ggtgagaacg
    33361 gcttcggcgg ccggttccgg atcggaagcc attcaaggcc tgggaagtta ttttctgacc
    33421 ttaccctacg gcggaatcaa gatggccgtc gccgtactcg gagccgtcgc cggaggaatg
    33481 gggttcattt ttaccggggg agacaaggct acggtcgaca aaatctgggg gccggctatc
    33541 ggcggggaat atgtcgtgac gccgcaacat ctcagggggg agaaagacct gcatttcttc
    33601 gggaacccgc ccgccaaata atcggttagc aggcgcccgc ccgaatacga cccgccttct
    33661 tcacttgcac caccggccgt ttctatcacc gtaatgggct cctctagcga gggcatcccg
    33721 gtggtgcatt tcaccagggc tggtggatcc caaccaccag ccctgcagct ctctccggag
    33781 aatagcggct aactgcctga aattcagtga ttcttcctct ctcccttcga ggcacatctg
    33841 ttgctgtagc ctatgaacaa atgggtcacg acattacacc tcgtcacacc accaaggagg
    33901 ggatcatggg agaattcaaa tcgggctttt tcatgagtga aaccgcctgc aatggccggg
    33961 tgctggtcgt ggatgacgaa ccggacattc gcaaagtcgt ccgcatgacg ctgcagaagg
    34021 ccggctacga ggtcatcgaa gcggaaaacg gggaaaaagc catagaggcc atcaacaccg
    34081 gagaaaaccg gctgctgctg gacgtgctca tctgtgacat ccgcatgccg aagatcaacg
    34141 gggttgaagc catcgcctac ttccaacagg aatggccgcg cgtcccgatt atcgtgctga
    34201 ccggttttcc cgacactgac atggccacct ccttcctgcg cagtggcgtc gtcgattacc
    34261 tggtgaaacc ggtggaaggg gaaaaacttc gcaccgccgt ggcgagggct atggagcaac
    34321 gggaactcgc ccagctctag caagggcccc tgccgtatcc gtcttcagca ctcgccgtcg
    34381 gggagagttt gatcacctcc ccgacggcga cgccctgtac cacaggggtt ttcgtccctc
    34441 cacccggagt caggcatgac actgaccagc acaaagatag ccatcatcgg cgcaggacga
    34501 ggcggcaccg cactgctcga gctcctgcat cagattcccg acgtagacat cgtgggcatc
    34561 gcggacaagg accccgccgc gcccggcctg caacgagcga aagatttgaa tgtgccagtt
    34621 gaggaacagg tgtacgacct cattcgcaac catggcgtca acctcatcat ggatgtgacg
    34681 ggtgatccat ccatggcccc cttcatccaa gccatgaaac gtcccggcgc cgaagtcttg
    34741 agcggagcca cgacgcggct gttatggaaa ctcgtccagc accagtcgaa actggaggcc
    34801 gaactgtttc aggccgataa actggctgga ttagggtcct ttgcggcggg catcgcgcat
    34861 gacatcaata acccgcttca gctcatcctc ggccttgcgg aaaatctcac ggacgagcaa
    34921 gatttgaccg tcgttcacga acaggcgcgc gacatcaccg aagccgtgaa acggaccagc
    34981 gctatttgcc gcgacctcac ccggtatgca cgacgcaacg ggacggggga agacatcatg
    35041 gtcaatctca acaccaaatt agatgaggcg ctgagaatcg cccgttacgc cgtgaccctg
    35101 caggatgtca ccgtcgtgaa acggtatgct gaggatgcct cggtcttagg gaaccctgac
    35161 gagttgcttc atgtcttcgt caatttaatt acgaacgccg ttcaggctat tcaggaccgc
    35221 ggtacgttga ccctgcacac gaacgttggc ccggacggac aggtcagcgc gtcggtgagc
    35281 gatacgggtt gcggtattcc gaaggaggcg ttctcgaaaa tctttgagcc cctgtacacc
    35341 acgaaacccc cggggaaagg cacggggctg ggactgtaca atgtgatgtc ggtgatttcg
    35401 aaaatggaag gacacatttg tgtcgaaagc gacgtcggtg tcggcacgac cttccggatc
    35461 gaatttccac aggtgcaacc gactacatca tgcgcgcccc tatgacgatc acctggcgcc
    35521 ccttctccaa tccgctggga tggggcctga aacgtaagct catcgtctcg atgctgctgg
    35581 tcggcgtggt tcccctgcta ctcggcctga gcatggcctt cctgcaaggc tccaaagaaa
    35641 tccaagtcgt cagtggcgaa agcttccagg ccttagccac ggaagccgcc cgcaaactgg
    35701 acttgctcgt ggccgaggaa gtcgccagaa cgtcccgcat cgccacacac ccggagatca
    35761 tccgacaact ggagcaccac cgagatctgc tgcagaccgc cgacaaaact ctgtccgcca
    35821 cgacgatcac ccaacaacga acccgctggg ccgcgcgcga tcccattgcc gtcaaggcca
    35881 ttaccgaaaa tcgcaccgcc gttctgctgc ggagctttta cgcgggggct caaaatgagt
    35941 ccgacctgct gcttcctcaa gtcgtacgcg ccgccacgaa aaagctgttc atcacggacg
    36001 tgcagggcaa tttgttcgct gcactcacca ccttcccgag tttttccaat gcggacagtc
    36061 cgtggtggaa aggaacttac aacaaggggg tggggcagct gtacatcgaa gatctgcact
    36121 tcgatgaaca ggccaaagcc tatgtcttca gcatttcgct accgatcatg gacagcctga
    36181 aatatgaagt ggtgggtgtc ctccatcgcg tcatcgatgc caaggaattt ttctctccct
    36241 cgacacatcc catccggttc ggcaagaccg gccacgtcat gctcatcgac agtcgaggaa
    36301 tcgtgttgag ctgtcccatt ctgcccaccg gcgttcggct atcagacccg gcgctcattc
    36361 cgcaagtgac cgtcagggaa ccggggtggg tcacagccga cagcgatggg catggcggac
    36421 aaggcacggc cattatcggg ttcgcgttgc tccctgaaac cagtcgcgcc acgaacggcg
    36481 ccctggaggt tggggcgtgg cacaccttcg tgtggcagtc ctccgacgaa ctgttctcac
    36541 caattcgaca tctcatgctg tggatgctga tcctggcctc ggttgccctc ttcctgctcg
    36601 ccacgctggg ctattttgcc gctagccgca tcgtgacgcc ggtccgtcgc ttacaggagg
    36661 cggcccgcct catcggccgc ggagaattgc aggagcccat ccagattcgc acgggcgatg
    36721 aactggaaga cctggcggtc gaattcaacc gcatgcacaa acaattggaa gcgtcctttg
    36781 ccggcttgag cacccaggtc gaagaaaaaa cgcaggaagt acagtacctc caaaaagcca
    36841 ctgatcaggt gctcgatgcc gtgccaacgc ctatcatcat ggtggatcaa caagggctcg
    36901 tccaatatat gaataaggcg tcgcgtgagg cggtgcaggc ggacagctgg tcatcgcgac
    36961 cgctgttcga cctcttgccc gtcgacgagg agcatcgcca ggcgctgcag cgggacttcc
    37021 gggcggtcga ggaaggcatg tccctggcgc cagtccgcca cgacgcgcct cccttgctga
    37081 agcccgcgcg cgatccgctc aatcagacga tcggtcccgc ccctgcttca tcccggcgcg
    37141 agctccgcat cggtccgcat ctgtatcagt atgagtggtt cccgctggaa agccgcagcg
    37201 gcgcgggaaa acgattcggg cttgtcctcc gggacaccac cgatgagagc cgcttgcagg
    37261 atcagctgat tcaggctgaa aaatcaggca gcttggacgt gctgaccgcc ggcatcggcc
    37321 acgaactcaa caatcccttg ttcggcattc tcggccttgg cgaagccatt caggaagaag
    37381 gggatctggc ccgcgccaaa ggctacgcgc aagacatcgt gggtcacggc cggaagatgg
    37441 ccgccatcat ccgcgacttc accggtgtgg cgactcgcga atcgaagagc caacggatgc
    37501 cggtcgatgt gacggctcaa ctggaacagg cactggctat cgtgcagacc tcacacgaat
    37561 gcctggggct caacgtccaa aaacattatg tccctgtatc ccccgtgacg gcacttcccg
    37621 accagctccg cctcgctttc atcaatgttc tcaccaatgc cattcaagcg atgaacgggc
    37681 aaggcaattt gtggctgacc acggaagagc aggacgatgt gattaccatc aagattcgtg
    37741 acaacggacc cggcattccc aaacagcatc ttgggaaagt ctttgatccg ttttatacga
    37801 ccaaagggca aggcgaagga tcgggcctcg ggctcaccgt cgcgcagcgg ttgatcaaga
    37861 aattcggcgg agaaatccgg atggagagtc ccccggaagg ccaaggcacc acctgcgtca
    37921 ttacgctgcc ggcagagaag gcggcggtac gaaaggagga gccatgcatt tcaacctgac
    37981 aggtgtacga tcatggacca tatggatggc gctctttctc gcttgtgtct ccacctattc
    38041 cttgcaggcg aaggacctca ccccaccggc aggcatctcc ccggaaaagg tcgcggactt
    38101 cgtccatgcc gtcctcgatg ccgaccggac gatttatacc aaccaggtcg tcaaccgcat
    38161 gcaggccaaa ggcatcgtct ccgcgatgga acattgggag catgaaaacg ccctcccgtt
    38221 acccgcgcaa ttcctgcaac actcgggcaa gctggtcgct gaatccggac gcggcatccg
    38281 ttaccggctg atcagtctct ggccgattta ccaacgcaat gcgccggcga cggatttgga
    38341 gcggcaggcg ctggagagtt tcgcgcagac acccgatcgc ccgttcaccg gcattgtcac
    38401 cagcggccgc aaacaatttt tccaagcggt ctatgcggat cgcgcgattt ccgccgcctg
    38461 catcaagtgt cacaatagtc atcctctgag ccctaaacgg gacttcgcac tcaatgatgt
    38521 gatcggaggg atgaccatta caattcctct cgactgaggg aagaggcgcc caacgagggt
    38581 caggacctga cgatcggcga ggcgggctgc agttggtatt cgtcgcggta aatctgcaac
    38641 gcggtgagga acaatccgac gagaatcggc ccgagaaaca ggcccagaat gccgtaggct
    38701 gccaatccac cgagcacgct gaacgtgagc agcaagacgg gaatctgcgc tccctggccg
    38761 atgaacagcg gcttcagaat ctgatcgatg gtcgatacca caccaatgcc ccaggccaac
    38821 atcactaagc cctttcccac cgtcccggac cacagaagat agccggccac cggcccccag
    38881 atgagcgccg tgccgccgaa cggcaaggga gccaggagaa tggtcagtgc catcaagacc
    38941 atgggaaaag gcacacccaa cacggcatag gccgccccgg caaggagtcc ctgcgcgaca
    39001 gccgtcagca caatcccctt gaccacggca cgaatggttt ggtccagacg gacgagaatc
    39061 ttgtccttat gggaagcctc gagcggaatc agcccgtaca gtgatgcgag ccaatgccgg
    39121 ccgtctttga agaaaaaaaa cagtgtaaac accatgacca gaaaatcggc caccagcatg
    39181 aacacgtcgc gcacgaggtc actcaattcc ccgacgataa atttgctgaa cgtcttggca
    39241 ctcgacaaca caaattgctc caggtcactt tcctgccccg tgaatcgctc gagccactcc
    39301 cgtgccacgc ttccaccggg aagtcccgag aggcgatccg ggagctgttg aacgcctccc
    39361 gaggcgatcc attcccgtac cgcacgttcg gcggacccgg cctctttgac taacatcaca
    39421 ctgaggacga ccagcggcac cacaaccaag gccagcgcgg ccagcgtgag gagtacggcg
    39481 gaatgcgatt ccttcccgcc cagccaggcc gtcaatcgga tatggagtgg gaagcagagg
    39541 tgggccagaa tgaccgccca caggaccggg aagacaaacg ggcgaaacat gagggccatc
    39601 tgatacagca gcgccagcag gacgccgaaa aagcaggctg caaaaatctg tcgtcgattc
    39661 atgagggggt atagatgttt gtacctgcgc cgttgatagc agaacccgcc cgtgatgtca
    39721 cgcgctggtt ttgcgatcct cgtgcctgta cggaagactt atcgctgagc aatgagacgg
    39781 aggggtaagc gtgaagaagc cgaatcagat agccgggagg tcgggagaga cgccgttcag
    39841 gcgttctccc cggccatggc caccgctttg ggacgctggg aggcaatctc ccgtacgtta
    39901 ccgggaagct tctgcgcgtc atcgccccac gacgtgacgc ccatatccga aaggccctga
    39961 aatttcgctc cgtcttccat cgagaatgcc ggcgcgtgca cctcgccgac gaggagcccc
    40021 gtcttcaaca attgaatccg ttccgtggcc cggaccgtgg ctttgatttt tccgctgctg
    40081 ataatggtgc cggccgtgat ggtgccctgc acgactccgt cttcgccgat caccaccgtc
    40141 ccgctggtct tcaaatctcc ctccagccgg ccgtcgatgc gcaccgtgcc ttcaacatgg
    40201 atttctcccc tgagcaacac gcctttggcc agcagggtga tattgtcgct ttccacaaag
    40261 ccgggtttct tcatcatcgg gagctccttg tcgcgggcag tggcggatga accggagaac
    40321 caattcaaca tgagccgagc ctacaccagc ttttcgatga cacctagaaa aaaccaaaac
    40381 tccgcttcac ccgttcccac atgccgcttc cgtgcacttc gcaatacacc gagggctccg
    40441 tgccttcgat aaacagctcc gccgaacgtt gcggacattt cgaggtggcc aactgcgagg
    40501 actgcgggtc gatgtcacgc gtgatcacgg ctggcggcat gacaaagtca cgcggtgcag
    40561 ccggcagaat ctggcgcaca aactccaccc acatcggtaa tgcggcctgt gcgccggtca
    40621 ggtgcaacgc gtcctcatca tcgaatccca cccatgcccc tacgaccaca tccggcgtat
    40681 agccgataaa ccaggcgtcg cgatagccgt ccgttgttcc tgtcttgccg gccacaatcc
    40741 cgcgcaggcc catcgctttg gccttcgccg cagttccgcg ttccaccacg cccttgagca
    40801 acgacgtcat gacatacgcc gcttgaggtg acaccgcctg atgcctagcc ggcgtgtgat
    40861 gccaggccgg atctccctcc gaggtcatta ccgcctgcag cgccgtggga gtcatgctga
    40921 ccccgtcatg ggccaggacg ccgaatgccg aagtcatctc caggagagac acgccggagg
    40981 tcccgagagc gatggaaaga ttatcggcca gcggactccg gataccgagt ttctctgcca
    41041 gggaaagaat gcgtttcgtc ccgacgagct gcgcgatctt gaccgccgga atattgagtg
    41101 actgttccaa cgccgttcgc agggacaccg tcccgtgaaa tttatggtcg taattctgag
    41161 gcgtccaccg gcccgtggcc gattcaaacg tcaccggctc atcgacgatc gaggtagccg
    41221 ccgttaccgg ttgcccgcca ttcagttcac gacgcgcctc catggcggcg agatacacca
    41281 gcggcttgaa gagcgagcct ggctggcgct tcgcctgcac cgcccgattg aactggctcg
    41341 tacgataatc gcgactgccc accatcgcca ggatgccgcc ggttttggga tcgatcgcaa
    41401 ccaacgcgcc ctgcaaaggg gtgggatggc ctttcaatgc gggataagtc gtctccagct
    41461 tgatgagttc gctttcaaga gtctgggtgg ccagtcgctg gagcacagga tcgagggtcg
    41521 tataggccct cacaccgtcc ggcaacggcg agccggtcgc ctcctccacc tgccgcaaga
    41581 gatagtcgac gaaaaacggc gcatcggcca gcgtgtcctg cggcggcatg acttgcaccg
    41641 gcgtcttgat cgcctgagcc caggcctcgt cattgatcag gccctgttca tgcagccggc
    41701 ccagcaccac atcgcgacgt tgcttggcca aggcggcatt tctcaagggc gaataggtat
    41761 tcggtccttt aatcatgcct accagcaacg ccgcctcttc cagactgagg gtatccaccc
    41821 gtttgccgaa ataccggtgg gctgcttcgc cgacgccgta gatcgacacc gaaccgacct
    41881 gcccgagata gatctcgttg gcataacttt cgagaatggc ttttttgggg tatttcgtct
    41941 ccaacaccaa ggcggcgatg gattccttca gctttcgccc gaacgtccgc tgcggggagt
    42001 aaaacaggtt cttggccaat tgctgcgtga tcgtgctgcc gccttgaata accgttccct
    42061 tggtgacatt cgtccacact gcccgtccca cggcgatcgg atcgatgccg ggatggctga
    42121 agaaccgccg gtcttcgatg gcgagcagga tatcgaggaa tcgcgcagag aggtcgtcgt
    42181 acgacaccca ttcccgtacc tgccgcgatg ccccgcgcag tccgctgatc aactgcggct
    42241 ccagataggc aggaaagagt tcgtcgccgg tctggatcga caggacccgc gtcacacgtc
    42301 cctgctccaa cagcaaacgg gccggcatcg gccgcagatg caagtcggaa aattcgtgca
    42361 gatagatatc aatctggccg ggactcacgc gaaactctcc cggtgcgcgc acgggcgtgt
    42421 cgaccgcctg ataacccagt cggctcaggc gctcgatcaa atgcgaggag accacatcca
    42481 agtcgggctt taatagaaag ggcgcgccat agatgaggcg tggaggatgt tcatctcctg
    42541 ccggcaacgc aaggctggca gaaaggtaca gtccataagc cagggcgctc ccgaggacga
    42601 gaaggagaat gcctgtcacc gccacaagcg cgcggagaag ccaccgcctt acgaatctca
    42661 ccaccgaatc ctcatgccgc cgagcatacg cgatcccgcg cataaaaaca cattcgccgc
    42721 ggcttccagc aatcggtctg gcttgcccct gcttgacaaa atgcccgtcc ttctcttaga
    42781 tgagccgcac aagaccatat ggctacgatc ttgtacctag aactatatga ggaatgaccg
    42841 gtgagtacga tacgcttctg cactgcccct gcttcctccg agaccgaccc gtcgtcagca
    42901 agccgggaaa cggtcgctcc aggtcggaac gcccgcacca ccgcacgcct gctcctcctc
    42961 accggcgcgc tgctggccgc ctggtcgacc atcgctccag gctcggttct cgccggaagt
    43021 ttccgcatct atgaccatag cgcctcggcg accggtcaag cctctgcgtt cacagctcag
    43081 gccgacgatg cctccgccgg ctattacaac cccgccggca tgacacagtt gcggggtgtg
    43141 cagctttcag ccggcaccac gctgatcgcc ggtggttttt cctttcagaa cggagcagga
    43201 acgcaggcca atggagacct ccgcggcagc gtggcggttc cgccgccgtc caatctgtat
    43261 attacggcca atctcaagga cttgggcatc ggctcttccg acagcactgc ggtcgggctg
    43321 gccgtcttca gccccttcgg caccttgact cgctggccgg acagcagccc cttttcgacg
    43381 gccaccacta aggccgcttt tgagttgatc gacattcgtc cgtccatcgc tttcagaccg
    43441 cttccggacc tcgccatcgg actgggagcg gatgtgtata cgttttcagg agccttcgga
    43501 gaaggacagg tcgaacggca atttcgctgg cccggcgggc tcggcattcc agccggcacc
    43561 ggcatggagt taaacggacg cgacaccgct gccggctgga atgccagcct gctgtatacc
    43621 ccgctgcgca atgaagatgg caaacccctc gtcaacatcg gccttatcta tcgcagccag
    43681 gccaccctgc acctcgacgg ccaattgctg gccggcggca cacatatcgc ggacgcacgc
    43741 acgaccttcg tagtgccaca ggtcctgacg ggaggcatcg ccctctggcc tgtccgcaat
    43801 aaagaacgcg aatggaaact ggaattggat gtggactata ccgggtggaa atcagtccgt
    43861 aacctggatt cacatctttc aaacggcatc acggtcgcca ctccggccaa ttggatcagc
    43921 ggttacaccg tcatgatcgg cacggaacac aagtggctca acccggcatc gcttcccgat
    43981 tgggatatcg ccctccgcgc cgggtactgg cattcacaaa aggcgatccc cgatcaaacc
    44041 ttcaaccccg ccatccccga cggcgacaac catgcggtgt ctaccggcgt cgggttcatg
    44101 tgtcgggaca acggacggtt tcttggggtg ataccctgcg gaggggcgag caaactcagc
    44161 cccaagggac tcggcatcga tctcgcttac caggccattt tgtatgaatc caggaccgtt
    44221 gccggcaacg tgaatccgac cgtcaatgga acctatcgca ccaccttgca cgtcggatca
    44281 ctgaatctcc gtgtgaattt ttgatgcccg ccttgtcatc gatcgtgcta gagtacgcta
    44341 tccgacggcg cgtgcggatc gtcgccgtct aacgggtacc gcgtgcagga cgcatctgtt
    44401 atcgagcgag gtcctcccat ggatcgcccg gcccacaccg atccggcact ggaactccaa
    44461 tcacaacaag tctctgttct gtaccgcaac atgcccacgg gcgtgctagc gagcgctgtc
    44521 aacgccgcca ttcttgccgc agtggaatgg agcgtggtcc cgcacacgcc actcctcgtc
    44581 tgggtaacca tcctctggat agttgccggt gccagggcgc tcctcatcta caactatcgt
    44641 cgaacgactc ctgccacgtg gagcagcgcc gactggcatc gctggatgct ggtcgcgacc
    44701 accatggccg gcgtcggctg gggaggcagt gtctattttc tgacgccgga aatccccctc
    44761 gcctacgaat tggtgcagtt gttcgtgctc ggtggcatga cggccggagc ggtctcggtc
    44821 ctgtccgtct cattccaact cttcttatgc tacgcgctgc cggtcaccct tcccacactc
    44881 gggcatctct tcttcagcgg ttatgacttt catgtgatca tgggcgtgat ggggacgttg
    44941 tttctcctgg ctacgatcca ctcgtcctgg accttcaacc atgtgctgct ctcctccctg
    45001 cggctgcagc tggagaaact cagcctggta cagtcgctca ccacgcgctc cgaagccgtg
    45061 gaacgtttga accgggacat caccaaagag atacatgatc gccgtgtgat cgaagagcaa
    45121 ttacgaacgg cccagagcga ccttgaacat cgcatcgccg aacgcacggc cgacctggcc
    45181 catgccaaca gccggctgca ggatgaggta cgggaacatc ggcggacgga aggggcccgg
    45241 ctctcgagcg aaaaacggtt caattacctc accgacaatc tgaaccaggg cgtgtggttt
    45301 gcccaagcgc agccgcctca agtcctttac gtgaacccgg ccttcgagcg aatctgggga
    45361 ctgccggccg ccagatttta tgagaacccc aaactttggc gcgattgcgt gcatccggac
    45421 gaccaacgga tggtgaccga ggtgtatgac acggaactga cccggcccac cggacgggac
    45481 gtgcaattgc tgtatcgcat catacgaccc gacgggcaaa cccggtggat tcacgatcgc
    45541 atggtgattc atcgcaccga gacgggcgag gtggacagtc tgagcggcat tacggaagat
    45601 attacggatg ctcgcgaact ggaagaccaa ttgcggcagg cgcaaaaaat ggaagcggtc
    45661 ggtcgattgg ccggaggcgt ggcgcacgac ttcaacaacg tcatgaccgt cattctcggc
    45721 tacagcgcag tccttcttca ggaactgagc cagaattctt cggcccggta cttcgtgcag
    45781 gaaattcagc gcgccggcga acgctgcgcg gcgctgaccg gccaactgct ggccttcagc
    45841 cgcaagcaga tgttgcaccc ggtctcgttg gatctgcatc gcgtgatccg cgatcttatg
    45901 gcactgctca agagcctgat cggcgaacac gtcacgatcg tgctccagct cgaccccagt
    45961 ccgcgatggg tcaaggccga cgccgtgcaa ctcgaacagg tgctgctcaa cctggccgtc
    46021 aacgctcgcg acgcgatgcc gcatggcggc accttgacca tcgatacctg tcaggtgctn
    46081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc agggacagaa attattcttc
    46201 tcgtggaaga cgagccgtcg gtccgcttgc tgactcagca cattcttcgc acgcatggct
    46261 ataccgtgca tgaagcggag gacgggttcc aggcgatgga tctcatccgg cgcaataatc
    46321 tgcacatcga tctattgatc actgacctgg tgatgccggg catgaacggc aaggagctcg
    46381 ccatgcggct gcgaagccac ttcgacgatt tgaaggtgct ctatatgtcg gggtacagcg
    46441 acaacctgcc ggttacgggc ggcgagagtc agggacagac aacctttttg cagaagccat
    46501 tctcaccgga agacttgatc cgcctcgtgc gcgagatcct gcactccgtc tccccagcct
    46561 agggcgacgg atgagtcccg gtgtgcgact ccaccggcat actgtgagac cgatgcccga
    46621 ccgggacgtc gatggtcgcc ggatgtgtaa tccactgagg cagcagcgat ccaatcacca
    46681 ttccacctgc ggccattaag gctccgacta actgcggcgg ccagacctga tccggcgtga
    46741 ctgtcccttc caacatcagc cacgtcgcga gcccggtgac aatggcgcag atcgctcctt
    46801 gcgtggtcgc gcgcggccaa tacaagcccg ccaggagcgg cacaaaggct gccacgagcg
    46861 tcactttgta cgcgctttcc accatcttaa aaatgctggc ttccgagttc agcgcgaaca
    46921 gcaagacgag tgcagtaaat ccgaccagca ccgagcgcat cacggccagc aggccccgat
    46981 cgctgatggt tggcattaag ctcttgacga tattctcgct gaaggccact gacggggcga
    47041 gcagcgtggc cgatgaacaa ctcatgatcg cggacagcaa agcgccgaaa aaaataatct
    47101 gtgccgccat cggcgtgtgt tgcacgatca gagtcgggag aatgagttgg gaatcccgct
    47161 ccagcaaacc ggccacctgc gccggatcga tcagtgtcgc tgaataggcc agaaacatcg
    47221 ggacgaaggc gaagacaaaa tacagcgttc cgccgagtac ggatccccgc accgcggtgt
    47281 cctcattcct ggccgacgta atccgctgaa acacatcctg ctgaggaatg gatccgaaca
    47341 tcatggtgac ccaggcgcct acgaacggaa tccacgcctc gatctgtgca ggcgggaaaa
    47401 agtccaattt gcccgccgcc gctgcatgag tgacgaccgt ccccactcca cccgccaggc
    47461 cgctgattac agacccgatg tagagcatgc cgcccatgat gatggtgatc tgcacgaaat
    47521 caagaatcgc cactgagaac attccaccga acgtcgtgta cgtcaatacg atcactgccc
    47581 ccaacatcat gccggcagac tggctcatgg ctccatccgt gacgacgttg aatacaagcc
    47641 ccaatgcctt gatctgggcc gaaacccacc cgagatacga agcgacaatg cagagcgtgc
    47701 acagcacttc aactgcgcgg ttgtaccgca gccggtaaaa atctccgatc gtgaggagat
    47761 ttaatcgata gagtcggcgg gcaaaccaca agccggccag aatcaagcag agactcgatc
    47821 cgaatggatc ggcgacgact gcacggagcc cgtccttgac gaaggtggcg gaaatgccga
    47881 gcacggtttc cgccccgaac caggtcgcaa agaccgtggc cgttaccacc ggtagaggca
    47941 gagagcggcc agcgacggca aagtccttgg tgttatgaac gcgagtcgcg gcatacagcc
    48001 cgatcccgac cgagcaggca agatacaaaa tcacgaagga gaggagcatg cggctatgag
    48061 cctgtcggtg agacgacatc cgccggtggc ggcaagcgag gggattgtag cgacagcacg
    48121 ccacttgcgc aatggcggga aggagcacgg cctacagcgc tggcgaaatc gtatggacga
    48181 cagcgtcatg caactatcat caacgaacag cgatggcgaa aaacgaccat ttcattgact
    48241 tccaatccca ccatgatacg aataggccat gcgttactca ctctacttcg cttgggtgct
    48301 ggccgtcctg tccatctgta tcctgacgac actggcctcg tttccggtac tcacgaacgg
    48361 cgagcgtcat ccggagccac ggattacggc aggaccggag aagttgcgga cgatcaagat
    48421 tcaccggctg cggtgcaatc cgttcacctc ctcctgccaa ctgtcccggc agaagtctca
    48481 gcactaagtt ttcgggccgg tcaccgtccc ttcgttacga gaggggaatg cgtgccttgt
    48541 tgacctccag ccactgcgca aatgtctgca atccgccatt gagcgcccgg gcaatcgccg
    48601 gttcacgggc tgcgcaaaag accgcattga agtcgcgttt aaactgaaac atattggcta
    48661 ggtcgtccgc ccccggaaac ccgaagttcc ggtacacttc cggcggcacc gcgttatagc
    48721 gcacctcgcg cccgagcgcc ttacctaatg ccgtggccat ttgtgagccg gtgagatgtt
    48781 cgcctgcgat accgacagtc ttaccgagat aggcctcccg cttcttgaaa attcccaacg
    48841 cacactttcc gatgtcgtca gccgcaatcc caggcaatcg ggcttcaccc atggggaggg
    48901 taaatgccag cgctccatcc ggaccaggct tcggacccat gccgaaatga atgaggttgt
    48961 cccagtaaaa ggaggtgagt aggaaggtcg tgggcagacc aagctgcttg aacagctgat
    49021 ccgcttcccc cttggcatcg aaatgcggca ccttatactt gcccatcaac gtcggcatcc
    49081 ggttgtctga gagcggaacc catcgacgcg tatcttcgag agtcgaccag atcacatgcc
    49141 gcacccccgc tgccttggcg gctttggcca tggcttcggc ctcggcgtac tccttttcgg
    49201 gagacaaatg cgcccagaaa aacgtgacgc aaaacgcacc ggcggcaccc gaaaaggctc
    49261 gcttcaggct ttcgacgtca tgcacatcgg cagccaccac ttccgcgccg agccgggcaa
    49321 gttctttggc cttgtctgaa ttgacatccc tggtcaacgc gcgcaccgcc agcccgctgg
    49381 ctggattggc caatatcgcc cgcaccaatc cgccaccttg catgccagtc gctcccacca
    49441 ccgcaattac ctgcttttct cccatgttct gttctccttt tgcgtctctc gtggtcacgg
    49501 gcaagcacac tttcccgcac ctctctgtca accaacgagg cagtcgtccg tttcactctc
    49561 tacgagccgg atctggtctt caccggcaca gccatgatag gctgtgaata gctagccagg
    49621 gaggagcgtt atggtacgtc ttacgacaca ccgattggaa tcccctcggc aatggtggac
    49681 ctctctcgca atgggatgca gtgtcctgtt gtttgccctt ccggtgtttg ctgcagaatc
    49741 atccggtccc tgggaatgct ccaactacac gggtgatgcg cacactcgat gcctgcaggc
    49801 cttcattgaa atccagcgcg aaaaaatctc caaactggaa gcggaagtcc agctccagca
    49861 aagcaccgtc ggacagttga aagatcaagc cgaccggcaa aacgccatga cggctgaatt
    49921 gcagcggcag atgtctcaac aatcgtccgc cccatcgact tatggatacg tgtcccccgg
    49981 aatcgcgcca ggattgctgt acggataccc gtccgtcggc ttcggcctgt atctcgggcg
    50041 cccctggatt tacggctctc cgttttacgc acgcccctat gcctggggac cgcgttatta
    50101 ccgtccctat tacggacggt ggcatcgccg ctggtaggcc gtttcgccac tctcgacccg
    50161 cagccgtggt gcacctacgg gttcatccgc ttcgctgtgg ttcaatcttc ccttcctctc
    50221 aattcacctc acaacggcgg atgacgcagg accgaactgt atgctaaacc atacagtccg
    50281 cattgcaatc ttccgactcg tcttttttgg gtacccgtgg gtcgcttgca ccacagcacc
    50341 ggcgttgcca gcgccattct tctcctggtc gtcggatact cgtccgtcag tgatgctcgc
    50401 cccgtcgaga aacgggcgaa accagtcgca gcacaagttg gctcagaaca gtccattcat
    50461 accgctctca ccgaaagttc gacaaccagg acccaggtgc tgctcgaccg cggagccaat
    50521 atcgaagctc gcaacgctaa aggagccacg ccgctcatta ccgcagcggg aagaggcaac
    50581 cttccgctcg tccttttact cttgagccaa ggggcgcaag tagcggcaca agaccatgac
    50641 ggaaacaccc cccttcacga agccagcttc caaagtcatt tgccatgcgt cgaagcactc
    50701 ctgaccgcag gtgcgcagac caccatccgt aacgaactgg gattcacgcc gctccaccaa
    50761 gcggtccgac gcttctggga gatgaacggg gaatcccatg cggatagatt gtctcgacaa
    50821 accgccgtga tccaccggct gcttcgctcc ggagccgatc ctcaaagtca ggacaccaat
    50881 gggagaactc cggtcgtgtt ggccaaggag agtgataatg ccaccctcca acaggcgttc
    50941 aggacggcat ctgttgacac gacaccggct accgacacgc caacacctcg ccgggcagct
    51001 ggagaggaat ctggagattc aagtcagccc ctcacagcag cacctcccca ccccaattcg
    51061 agcgcggttc tcagtcccgc tgaggtcagc gtattctcct tgcctccgca tcagcccagg
    51121 gaggcgccgg ctgtcacgct accggcacag agttcacccg aggcgcccac tccggctgga
    51181 tccccctcca cccgtttacc agagcacagg ccggacgcga gcgctgatgc gggtggcgnn
    51241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct cggtctggga tggactcata
    51361 atctcggcgc gcggcgagtc gaatccgttt ccgtcaccaa ccgcatcgta cgcatcgacg
    51421 acgaacgcaa cgacctggtt cgcgcgatgc ccgaagttca cctctggatc gatcgctggg
    51481 atgagcaacg atggagttgg ggaccatttc tggccgtggc tccgggatcc cggatcatcg
    51541 acgccgtggg aggcgggctc atggtgggct accggccgca tcgcagcgat cagtacagtt
    51601 tcaatttcgg tatcggcggc atcctggatc tggatacgca ggttctggga gacgggcttg
    51661 tcgccaacga gccgttgccc ccgcgggaga ccagcgcccg taccaagcac acgaccggcg
    51721 ccgggcttct cgtgctcttt tcggtcggat gggatctttc agcgccccac caggtttccc
    51781 cgcctggtcg aaaataaacc gtcgctgccg tgcgactcac gtctcttgac gatcccggat
    51841 tgacggcact catcggatct gactaaggtc gaacgctcaa ggagaatcaa gcatgacccc
    51901 acagccgcag tttccaaacg aacacaccga gtcaggcgaa tttcaacggc aagaagacgc
    51961 ctttcgccaa tgggtgactg acgacggacg atcagggttg ccggccgcag ccgggcgcta
    52021 tcatctctat gtgtcccttg cctgtccctg ggcccatcgc acgatcattg tccgcgcact
    52081 caaacagttg gagtcggtcg tcggcatgac cgtggtggat cccattcgcg acgaacaggg
    52141 ctgggcgttt cgtaccggcc agggccactc catcgatccc atcaatggat ttcacttcct
    52201 cagcgaggcc tatcacctga ccgaccccac ctacagaagc cgcgtcactg tcccggtcct
    52261 gtgggatacc gtcacgaagc gaatcgtgag caattcggat gacgatctca tgcggatgtt
    52321 caacgcggag ttcaaccgct tcaccatcag tacgctggat ctctatcctg cagcgctgcg
    52381 cgcagacatc gatgagatga acacattcct gtatgagcag gtgaacaacg gggtctacaa
    52441 ggcaggattc gcgacttctc agcgagggta cgaacaagcg gcacggtcgc tctttgctgc
    52501 cctggatcaa ttggacacca gactcagcca acgccgctat ctcttcggcc cgcaattcct
    52561 ggaatctgac tggcgactgt ttgtcaccct catccgcttc gacgccgtct atcacggaca
    52621 cttcaagtgc aatgtccggc gcatcatcga ctatcccaat ctctccgggt acctcagaga
    52681 cctctatcag gtacccggca tcgcggacac ggtgaatttg gatcatatca agcggcatta
    52741 ctatgtcacg catgatgaca tcaacccgac ccagatcgtg cccatcggac cgcaactgga
    52801 tcttgagagc cctcacggac gagacaagtt ctcctgagcc tcacttgacg aca
//
LOCUS       DAOQDS010000026        50707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_214_length_50707_cov_21.082524, whole genome shotgun sequence.
ACCESSION   DAOQDS010000026 DAOQDS010000000
VERSION     DAOQDS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 50707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50707
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214_length_50707_cov_21.082524"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="PK589_08200"
     CDS             complement(<1..1784)
                     /locus_tag="PK589_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19621.1"
                     /translation="MARRSSQNDSLADPAGTGASPVFPDPTPLLVASGLDADEVVRIL
                     GPYGLRNPTEADTNIQSMAGEPHTRRLLAGILSRLLAEVARTADPDQALNHWERLFAG
                     SANRSSLLQYLQASPRMLGLLCTIFGNSDSLAFALIRDPMLVYWLAEEDVLTTAPTRE
                     GMVAALRQSLGSLKATELKLDVLRRVRRREMLRIGVRDLLRLATVEETVGSLSDLASV
                     LIHAAYEIVDKDLKTQHGTPMHQRPDGTWIETEFVVIGMGKLGAHELNYSSDVDLIYV
                     YASADGETRKAKGNRQASIKPLSNEEYFEMLARSLTKALAEQTREGAVFRVDLRLRAE
                     GTVGQLARSVDAYAKYYAQRGQVWERLALLKAWPVAGSPAVGQAFVQMAEGFVFQSDL
                     QPLTRERALAVIRDVRSVKEMIDEKIAVRGHQHRNVKLGTGGIREIEFLVQTIQVLIG
                     GAVPDILDRKTVGALHRFCRHRFVNEDDQQQLTEAYWFLRDVEHKLQMMHDLQTHALP
                     DSHEELQRCAIRMGYYGESRKRAMEQFLADRQRHTAVVHQTFRSLFYHPETSDLLSAV
                     VAAAEGHSRRNPSRLEKETGPAGPPPDRS"
     gene            1855..4131
                     /locus_tag="PK589_08205"
     CDS             1855..4131
                     /locus_tag="PK589_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19622.1"
                     /translation="MLHANTDRDQILATLAPLCPGIETDILQDFTSRMDADYFAAFSP
                     KLLAEHLSLAALLTPDHPCEVRFAKLDRKRLTITIVAYDYFSEFATICGLLSAFGLNI
                     EEGRIFTSAESEPARGGRQNSPYGTRPRPQGRPGLTRKKIVDVFTVTPTEGEPFTADD
                     QKRLAEQLSRMIILLDTNEFDEARQQVNRQLIEYLGTRRSSFSGLLHTVHITFDNSQS
                     ATDTIMDIRSDDTPAFLYAFANALAMRNVYISKAQFAIEEGKLHDRFYVRNRFGQKLL
                     DPGDQEQLRLTAVLIKQFTHALTWAPDPAKALESFDQFLDLVLEGSRQAGKKQAWDFI
                     NDKKTFPLLARLLGASDFLWEDFLRRQHVNLLPLLKDYRDAPLIKPQATLRKELNRLI
                     AKAKTDEARKEALNRFKDQELFRIDMKHIVEPGTNLPDFSLAISELAEVIVERSLLDC
                     QAKLTKRYGSPRLANKKPCPFAILGAGKFGGRELGYASDIEVLFVYGGPGHTTGKDGI
                     ENSEYFERLAQELLQWIEAKQEGIFHIDVRLRPHGGKGSLSNAFDEVCKYYSADGLAA
                     PFERQALIKLRHIAGDAALGRKVEAHRDSFVYSGAPWDLTTAIDLRRQQVKQLVEPGQ
                     VNLKHSHGGIVTLEYAIQYLQVMHGHRHASLRTPNTLRALAALIDADLIPRTTGENLR
                     KSYLFIRMLIDGLRMVRGNTKDLVLPPPDSDEFIFLARRVGYQTEDWQAGAKHLLSDI
                     EQHMKQNREFFEKMFGKV"
     gene            4333..4761
                     /locus_tag="PK589_08210"
     CDS             4333..4761
                     /locus_tag="PK589_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19623.1"
                     /translation="MLTRVLAGLLLSVVLASCGQGDGGAGSGIKPVGEWAKIGETDTY
                     IYYADYASIKKADETVVMLDLFDYKSAQSEGGGAPALSKATEREYDCQNKKSQPLKSS
                     WYSEGMGAGTVVRSTGTSNQWSSAAQGTATGGLLKAACGN"
     gene            complement(4834..6003)
                     /locus_tag="PK589_08215"
     CDS             complement(4834..6003)
                     /locus_tag="PK589_08215"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNJ19624.1"
                     /translation="MHELANGRSVRKWPLSIPAGKTSLPLMFILSFLVLAGLPWAWGA
                     VDAAEVPTTPGIVKWHPGHYYAPMTFMRANANVMTQIYAELKATPALRGLQVRFAWPE
                     LEPEEGRYDFKGIEKVLAELAPLNKQLVILLELKSFNPQILPMPTYMRTEAYEGGAFP
                     FSTYGQSIPRGYNLKLWNSGVHDRLVALLTELGKRFNGHANFEGIGLPETALGQPIEL
                     LSSHDTEKFYDNLLDVQRQMRAAFPNTVTYQFVNYPREILPGFIEHMRTIGTGLGGPD
                     SFLDDPGLNFDHPNKPKGIYSYYPQMSGLIPLTPSVMQANYDNTRHDGKGRVPTVAEL
                     LAFGRDRLRANYLFWTRAPGHFQQVLEQMQRIPLQGNPSGGLDAVCPKAYVSCVE"
     gene            complement(6321..7097)
                     /locus_tag="PK589_08220"
     CDS             complement(6321..7097)
                     /locus_tag="PK589_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ19625.1"
                     /translation="MAKRTKSSQEPADLLSRHIEEIRETLAAFQPFLSRRKATASLEG
                     FDEQAEEVLSETFGGASDELEAYYYAKLGEAAILPEEAQEAGTHNLERESLHQRKQVL
                     ESCLAQLELKKSTRVGRDWRRTLGERIDGRTVAEFMSSEVRSVHKGASIKEAGRLLQK
                     WRIGSLLVDDGSRYLGIITDTDLSRKAVAKGLDPNSTTVMSCMSKAVVTIEDSEPIKE
                     ALRLMKKEGIRHLPVTEDGTIIGVLSVGDLLRAYQKMLEL"
     gene            complement(7160..8764)
                     /locus_tag="PK589_08225"
     CDS             complement(7160..8764)
                     /locus_tag="PK589_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HNJ19626.1"
                     /translation="MNKIEAIKTERDGLAVRDMLEHYAHAGWESIPEDDIQRLKWYGL
                     FLRNPTPGFFMLRVRMPGGQTTSVQLQALADIALAYGNGVMDVTTRQQVQLRHLTIAH
                     VPAVFAKLDEVGLTSMQTGMDSVRNVMTCPVAGLNPNELLDGTEIVRAINHEVLGNPA
                     YTNLPRKCNIAVTGCTDNCLHTETQDIALVPAYHDLGHDKCFGYNVLVGGKLGSGGYR
                     IASSLDMFVNPAEAFDVCRTLLHIYRDHGPRENRTQARLAFLVEAWGESRLRHEVEVR
                     MGRALPSAGVDARAAGERDHIGIFRQKQRGMNYIGLKILVGRIKAGDLRNIAALAERY
                     GTGEVRLSPAQAIVIPHISDRVVGELAEEPLVKQFAYNPSPLYKGLVSCVGSDYCNMA
                     VIETKRRAVETAHILERKLEGLKPITLHWSGCPAGCGNHLVADIGLLGKKARVGGKVV
                     DAVDIFVGGRSGPDPKLATKIMEDVPCDKLPAVLENLMPYHTRDKMHRVRGKAVAKST
                     ASAKSASSANAADGAISGLRPQPFPA"
     gene            complement(8761..8958)
                     /locus_tag="PK589_08230"
     CDS             complement(8761..8958)
                     /locus_tag="PK589_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19627.1"
                     /translation="MKTNEETVVLTLLRRQAPQSFEQLMALSGLSASQVLLVVDRLSR
                     EGTVVLRKSGLDYSVALGSTI"
     gene            complement(9008..9451)
                     /gene="cynS"
                     /locus_tag="PK589_08235"
     CDS             complement(9008..9451)
                     /gene="cynS"
                     /locus_tag="PK589_08235"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HNJ19628.1"
                     /translation="MMEKEAMRKAIKAQRLKKKVTIAQVAKKVGKNPTFVAAALNGNH
                     RLVPADAAKVGALLGLNQEQVAALSAFPIRADFPNLTDPFKYRLMEVIGVYGDSLREM
                     ANEMFGDGIMSAIDFTLDMEKVTGSQGEARCKITLNGKWLEYKTF"
     gene            complement(9502..10332)
                     /locus_tag="PK589_08240"
     CDS             complement(9502..10332)
                     /locus_tag="PK589_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNJ19629.1"
                     /translation="MHTADHERIAGVAAKKWMFLEQAPGGYFILSSLAGIYLGFGIAL
                     IFSLGGPLAAAGSPVVKLVMGVSFGIALTLVIFAGSELFTGNNLIGAIGGLSRSLSWT
                     QVIQLNAWSWFGNLAGSMGLAWLIVESGVFAKGPSADLIEKVAAAKMSLPAWELFVRG
                     ILCNWLICLAVWMTGRTTNDTAKILLIFWCLFAFIGTGFEHSIANQSLLGMALFLPHD
                     VTVTWAAFWYNQLFVVLGNLVGGGLFVGGLYWMVSPYRVAEVPVTPVSSPATSAVVGS
                     "
     gene            complement(10650..12059)
                     /gene="glnA"
                     /locus_tag="PK589_08245"
     CDS             complement(10650..12059)
                     /gene="glnA"
                     /locus_tag="PK589_08245"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNJ19630.1"
                     /translation="MNVREVLDFAKKNKVQVVDMKFVDLIGTWQHFTIPVSELTEGLF
                     KDGSGLDGSSIRGWRAINNSDMLLVPDPATACMDPFCSVPTLSLIGNVVDPITREMYD
                     RDPRFIAQKAEKYLQSTKIGDTSYWGPEAEFFIFDHARYDQTNHSAYYHIDSDEGVWN
                     MGQDGVNLGGKIRHKEGYFPVPPTDTQQDIRTEMILEMEKAGIATEKHHHEVATAGQA
                     EIDIRFDSLLRTADKMMMFKYIVKNVARRHGKTVTFMPKPIFGDNGSGMHTHQSIWKD
                     GKPLFAGKEYAGVSQMCLNYIGGILKHAPALAAFTNPSTNSYKRLTPGFEAPVLLAYS
                     SRNRSAGIRIPMYSPSPKAKRIEVRFPDPAANPYLAFAAMLMAGLDGIENKINPGEPA
                     EKDLYDLEAKEAAKIRTMPGSLDEALNHLEKDHQFLLKGGVFSEDLIEAWIGYKRTKE
                     VDTMRLRPHPYEFFLYYDV"
     gene            complement(12109..14793)
                     /gene="glnD"
                     /locus_tag="PK589_08250"
     CDS             complement(12109..14793)
                     /gene="glnD"
                     /locus_tag="PK589_08250"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNJ19631.1"
                     /translation="MSHALDAGFHMVQDAAAIPVSQLLAEQRQAIQQRLLAGASGDEV
                     VAATTDLVDGLIIGRYRTAARTGGEALTTAGFQHCCLVAIGGYGRRELAPHSDIDLMF
                     LFHQDAAKVIPELVKQVLHPLWDIGFQVGHSVRTIQDCIDLGLADLTVRTSMMEARFL
                     TGSPELFQQFHARYVRKVVSSGFDGYLEQKVAERQREYQKFGETVYLLEPNVKKSKGG
                     LRDLHLLQWIGSARFQAPTIRELSDRGILSQSDYRSIKEAREFLLRVRSFLHIRAGMA
                     QEILTFDEQVWLAKHLGFTDRPHLLAVEQFMQQYYRHTMGLHVALMRFVERCRRRSLW
                     QRLVRWLPSPRIQQYFSVDGEALTVPVELRSQVLVQPTLLLTLFNLARSRQLSIDPSL
                     LEDIHRHAETLSVEQFHTPEAGQTFLSILAGPGTARTLEAMHRAHLLEKLVPAFSRVR
                     GLMQFNQYHKYTVDEHSLLAVAKAETLADHSGVLGEVYREIKRKDLLHLALLLHDLGK
                     GHEEDHSEVGKRLAEETAARLGFDEPDTRTLVMLVHRHLLMAHTAFRRDPYDEKVLLP
                     FAREVGTPEVLRKLLALTAADIAAVGPDVLTKWKESLLVELYLRAMQEVSGERETADE
                     PQRLARIADDVAGQSQGDHNLPADKTWICSELEQFPLRYVYGTSPLRIAAHLGAIRRL
                     QEGGVVVETEFNAPLGTCEYAVIAHNDLIPGLFSKIAGVMAAGGLQILDAQIVTRKDG
                     VVVDTFQVADPDYQGAPPADRCESIGATITEVLTGRQSIDAVMRRGARLNLGRSLPAH
                     RQPAEVRIDNETSDRFTILDVFADDRQGLLYIITNAIFQLGLSVHASRISTRLDQVAD
                     VFYVTGMDGKKVEDAGRLETIRASILNEIELFLGAHAA"
     gene            complement(14796..15134)
                     /locus_tag="PK589_08255"
     CDS             complement(14796..15134)
                     /locus_tag="PK589_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNJ19632.1"
                     /translation="MKLIEAIVKPFKLDEVKDALLEIGIQGMTVTEVKGFGRQKGHKE
                     TYRGQEYTIEFVPKVKIEVAVNDGQVQRVLETITRAAKTGSIGDGKIFVRDLASAVRI
                     RTGETGESAL"
     gene            complement(15181..16509)
                     /locus_tag="PK589_08260"
     CDS             complement(15181..16509)
                     /locus_tag="PK589_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNJ19633.1"
                     /translation="MKASAQWKTFLVGLAGALTLIGGLGAEQAWAENAPLKADSGDTA
                     WVLVSSAFVLAMLMPGLALFYGGLVRTKNVLGTIMQSIMILSVVSLLWILFGYSLAFG
                     PDKGGVIGGLDWIGLSGVGSQPHAVYAPTIPHQAFMLFQLMFAAIAPALITGAFAERK
                     RFTSVVLFAAMWSVLVYVPLAHWIWGGGWLAQLGALDFAGGAVIHISSGAAALICAIV
                     LGKRRGYGTDYMAPHNLPMTLLGTGFLWFGWFGFNGGSALGANGVAVSAIIASHAAAC
                     MGAISWCGAEWMHRGKPTVLGLASGAVAGLATVTPAAGYISPLWAILIGLVAGMSCYA
                     AIVWKGRFGYDDSLDVVGIHGVGGVIGVLATGLCASKVVNPGGADGLFFGNPGLFGVQ
                     LLVVVVTTVFSLVGTFAILKLVDAMTGLRVSPEEEATGLDLSQHNERAYS"
     gene            complement(16587..17465)
                     /locus_tag="PK589_08265"
     CDS             complement(16587..17465)
                     /locus_tag="PK589_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19634.1"
                     /translation="MNMLVGRKARAWVLGLALMAGGGGGALVEAQAATTDDLMDTTKH
                     PVSVTMAMQADSFFGFNPSIYGTYGLTDNIALAFNFTYWTMISGVGVHDNAPWLETDV
                     GLNFTFLEKRLSVTPMIGFVHGSLLSSRGGFFPNGGGSVNERTTAFEGAVPNIIANYI
                     DNRFEGEFYMGYYKAIRSEGGSGGGGAIGCGNPGGTNCLGPIQGGGRGTWDFLHYWVS
                     AGVRVNSMWSMGAHYEHLLTTRDSSAPGAAQDYYRWIGPYVEMKLPGNMAFRFTVGKD
                     LTDNQDFYKLKFTKTF"
     gene            complement(17600..19372)
                     /locus_tag="PK589_08270"
     CDS             complement(17600..19372)
                     /locus_tag="PK589_08270"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNJ19635.1"
                     /translation="MMRVLRVAMAQINPTVGDIVGNTALIKTWINDARRAKADIVAFP
                     ELAITGYPPEDLLFKPRFIEDTQRALKAVAAEAHGLVVVVGYVGREATGVTPSEAEAV
                     SPAGRHDLYNAAAVLCERRLVANYCKRLLPNYGVFDESRYFRPGTRLPLLVLNGTTIG
                     VNICEDIWFPEGPTRAQAAAGAEVIVNINASPFHVGKSRLREQVLATRARENRVMVTY
                     TNTVGGQDELVFDGRSVIVDHTGEVVARGKSFEQDLLIADLDVAAVGRARQGERRARP
                     LPPRVAALVDRVAVRVPARKSSSIAVPAMESPLDRLEEAYRALVLGVRDYVQKNGFKR
                     VVIGLSGGIDSALTAVIAVDAVGADNVLGVFMPSPYTSRASREDVADLAQRLRIQVDT
                     LSITTTFKSYLRALSKPFSGHRPDTTEENLQARIRGNLLMAYSNKFGHLVLTTGNKSE
                     MSVGYATLYGDMAGGFAVIKDVPKTMVYELSQLRNLNGLEPVIPKRVLERPPTAELRP
                     DQKDEDSLPPYSVLDPILKAYVEEDRALEDIVAMGFERETVARVMIMVDRSEYKRRQA
                     PLGIKITHRAFGKDRRMPITNGYR"
     gene            19780..20649
                     /locus_tag="PK589_08275"
     CDS             19780..20649
                     /locus_tag="PK589_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19636.1"
                     /translation="MRLRKAWLLGLTCLLLVACSATSPLIPVGEGPLGTVSLERLASR
                     GTTARYGSPQSAFQASHPASVSASVISRLLSGLSVSGLERPGAALRQERYPLFTQEEM
                     EFFSPLIVKALSQAGPDQRVRFTLQDDGLITQGTLYLHKTTLRVSLSHYRASQGQSDT
                     RPTALTLSFTPSEALVRDDVPQSWMIIEPEQPRVAVSLEVLNELRAAVSAPTANKPVA
                     VIPVAPAPAASEQTNLQQELQATKDVVVKQAQELQQLKTELESLRRELAERDSTTPKA
                     KPKAAPRKTVPTP"
     gene            complement(20924..21298)
                     /locus_tag="PK589_08280"
     CDS             complement(20924..21298)
                     /locus_tag="PK589_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19637.1"
                     /translation="MKAMRKAWCIACLVTVAACTTGQYQPLNGADKDEVEQLRENVYR
                     VEYRVSSFTSQEQLDSYLRRRCAELTLREGYNFFHLAQRADVLALSRRTSMTVTMYKG
                     KKPSGADDVYDAKEVLAGPLAQ"
     gene            complement(21760..23019)
                     /locus_tag="PK589_08285"
     CDS             complement(21760..23019)
                     /locus_tag="PK589_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HNJ19638.1"
                     /translation="MASKRTVPQLEQALREKTREVDVLHRISESISSTLDLESVLRHI
                     VDVVVEATKADACLLYLLSDNRDELILRASKNPHPKLIGRITIGLGEGITGWVARERT
                     RVVIPNSASDDSRFKFFHNLPEDRYQAFVSVPITTKQEVVGVINVQHKRSRRYRPDEL
                     ALLSTIATQVGGAIENARLYDQMTRKALQLDTLSQVSETVSSNRLMEDVLQLIVTMTA
                     QMMGSKICSIILLDQPTGELRIAATQSLSEQYRRKPNVKIGQSISGRAVQDRRPIIVP
                     DVTKEGSYMYPDMAAKEGLCSLLCVPMLVREKAIGVINTYTSKPHTFTSEDVKLMQAI
                     ANQAAISIEHTTLLEKSFEMQEALQVRKLLDRAKGYLMRSKRLSEEDAFKLIQRQSMD
                     LRKSMREIAEAILLAGEIEDRADKNRG"
     gene            complement(23027..24184)
                     /gene="aroB"
                     /locus_tag="PK589_08290"
     CDS             complement(23027..24184)
                     /gene="aroB"
                     /locus_tag="PK589_08290"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNJ19639.1"
                     /translation="MATSTPDMPRDTIHLELGARSYDIQIGRGLLKEIGVEMTRLRCA
                     GKVGVVTDRNLAGHYLKPVIRVLKAAGYTVVPIVLPPGERTKTLRSIGTVMDALVNAR
                     FERSSTLLALGGGVIGDITGFAAAIYQRGIPFVQVPTSLVAQVDSSVGGKTGVDHPKG
                     KNLIGAFNQPRAVLIDPATLRTLPEREWIAGLAEVIKYGVIEDESFFKYLEEHIAPIL
                     MLSDAPVAHIVKRSCEIKAQVVSEDEREADRRRILNFGHTIGHALESLGGYRGLIHGE
                     AVAVGMVYEADLARHLGYCHEDVVTRLRALVAAAGLPVRVPKVPFRALWDAMQQDKKV
                     SAGTVYCVVPERIGSVRIVPLAKEQTRAWYDTVSSDNTRSRQFIPNNKRRR"
     gene            complement(24454..26403)
                     /gene="pilQ"
                     /locus_tag="PK589_08295"
     CDS             complement(24454..26403)
                     /gene="pilQ"
                     /locus_tag="PK589_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HNJ19640.1"
                     /translation="MKQTQVGVHPLLSVTVMAGLLGLAGYVQAAAPGETTGLAGFTQA
                     TDQATSAEFTAAKEEHSTGATPTATLLTSVEAKPEGGRVALILTGNGVFDHTVKMVGD
                     RRMVLDMPHIQSQGRQTQLAVGHALLSKVRFGYHADKVRLVLDLAQPAEHSVTSVDGR
                     LVLMLEPKSVPTNDVKLAESEASPSAEQTAARSMVVSHKTPHALFRVQPIQLTAESVQ
                     KEDRKPETNEVVNGSSRFVGRRISLDFQQADISNVLRLIAEVSGFNIVVGEGVKNKVT
                     MKLANVPWDQALDMLLKMNALGMIRQGNIVWVDTLQNIAKQQDEEARAKDSKAKAEPI
                     VTRVFYIRNLNATEVQTSLRQNLSPRGTMTVSAASNALIISDTESKLEVLRQLLDGVD
                     LEVPQVQIEARIVQADTTYTRSLGVQWGIQNVNQLGASSGTSAFKTGTTGAFGAQVSD
                     FLINLPANPGLPSVPGAGFSIGKTDGAMLDVRLSAGELLGLTKVIAAPKITTLDKRDA
                     KIAQGESIPFQTTSLQGTQTTFVDANLELNVTPQITSRDPKEIGKQILMKVRATRNAV
                     GARSNPAGPSIDRREATTQVLVRDGETMVIGGVFVDTQSNNVAGIPYLSRVPVLGWLF
                     KNKTENVSKQELLIFLTPTIVRSAT"
     gene            complement(26400..26999)
                     /locus_tag="PK589_08300"
     CDS             complement(26400..26999)
                     /locus_tag="PK589_08300"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HNJ19641.1"
                     /translation="MNTRTCWKMSIGAAVLVIGGTAMPVESKSLPHLRQIASMRPADS
                     LKVMPLPEGQKPAPAMESPAPRVEASVSQPGDSLSMEFSGTSYDPSGRRDPFLPMVQL
                     GQQVEQDASLPPLQRVGLTELSLIGVLWGNYGYTAMVQTPDGKGYSIRRGTRIGPNNG
                     VVSSITERGIIVQERFTDVYGNKQEREYVKLLHPKEGTE"
     gene            complement(27041..27664)
                     /gene="pilO"
                     /locus_tag="PK589_08305"
     CDS             complement(27041..27664)
                     /gene="pilO"
                     /locus_tag="PK589_08305"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNJ19642.1"
                     /translation="MSLNAISLDSLRSIPTGQKVALLGLLVAGILVGFYFYVVDPKTV
                     ELEAVQGQVAQLDTEIQNLTLKVKHLDELVAANKQLEIELAAKKERLPPEEEAVMLLK
                     QVSDLGLRLGLDVKLWKPGTQAEDPSKLFIRMPINVEVAGGYHTAAIFFDRISKLSRI
                     VTVQDVRIGAARVDQGRVVTQTVFDLVAYAAPQEKKPAAPVPAAKPK"
     gene            complement(27666..28220)
                     /locus_tag="PK589_08310"
     CDS             complement(27666..28220)
                     /locus_tag="PK589_08310"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNJ19643.1"
                     /translation="MIRINLLATGPRSRKAKPQWDVRAEALIGVGVLLITLTGCWFYA
                     SSLDEDIQAKQSEKQLKDKQVAQLKEQVKAVQDFEDKKKQLEAKNRIIDQLEKSRTGP
                     VKVLDHVSQSLNPLKVWLVRLNLKGNSVELEGRALTNDDVVEFVNNLRRTDQFGAIKL
                     MESRAGLDNKMNTYQFRLDLSMKG"
     gene            complement(28217..29314)
                     /gene="pilM"
                     /locus_tag="PK589_08315"
     CDS             complement(28217..29314)
                     /gene="pilM"
                     /locus_tag="PK589_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNJ19644.1"
                     /translation="MLASLKHLTDMDFLSMFSPQKQLLGLDIGSSSIKLVQIKEHRGR
                     YTLQKFGVKELEPEVIVDGTVMDEGRVVSAIKELLAEHNVKLKQVAVSISGHAVIVKK
                     ITLPPMPDEELDAQVKLAAEQYIPFDINEVNLDFYVLPPSENPDEQTEMQIVLVAAKK
                     DKINELTELVKAAGLVPIVMDVDAFAVENMYGVTSPSTQEDTTILVNIGASVMNVNIV
                     GGGVSLFTRDIPLGGNRYSEAVQREMGVSYEEAEQLKKNDGDDDHTLAAVMESVNAEV
                     ASEIARTIDYFKTTSSESEIARVLLFGGGAKVKGLAQQLRDRMHVDVEIANPFNEIDT
                     SQCNVDPEQLAEMGSLAAVGVGLALRTVGDR"
     gene            complement(29328..29543)
                     /locus_tag="PK589_08320"
     CDS             complement(29328..29543)
                     /locus_tag="PK589_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNJ19645.1"
                     /translation="MKKVNDGQKKKLYKTNEVCEMFDISRATLFRWEREGLITGPPRD
                     WRNWRLYTAENVEQIKHVMGGGRKDVA"
     gene            complement(29679..30545)
                     /gene="rapZ"
                     /locus_tag="PK589_08325"
     CDS             complement(29679..30545)
                     /gene="rapZ"
                     /locus_tag="PK589_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNJ19646.1"
                     /translation="MAALNLVVISGLSGSGKSHALKAFEDAGYFCVDNLPPALLPTFV
                     ELCHQQGGEIKNVALGIDIRERVFFGDLAKVLAELKSQGHALQLVFLEAREEVLVRRF
                     SESRRPHPLLPHMPVVEGVRFERERLSELRKHADRVIDTSTLTVHELRDLLIRQFRQE
                     TTARRMTISLVTFGYKFGVPYDIDLLFDVRFIRNPFFVPELKALTGEDLRVQQYVFGD
                     ETAGQFLEHLQKLFAFLIPLYERDQRSYLSIGIGCTGGRHRSVTIALRLQAQFAALGY
                     DVSVTNRDLQKS"
     gene            complement(30532..31083)
                     /gene="raiA"
                     /locus_tag="PK589_08330"
     CDS             complement(30532..31083)
                     /gene="raiA"
                     /locus_tag="PK589_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNJ19647.1"
                     /translation="MRLMITGRHVVVTPALREYIELRMERLDRYGLKLGTLQILLSVE
                     KFHHVAEVVGVVQGRRLQAKTTTEEMYASIDEVVDKLGAQLRKLKERKVNHKFGDQPR
                     VRAVARKTPRAKDDGMEVVRPRLEALTIEEAVETLNGEKLPLLMFVNALSGSIQVLQR
                     LPNGRLSLIDPASDRARVAHGRA"
     gene            complement(31190..32707)
                     /gene="rpoN"
                     /locus_tag="PK589_08335"
     CDS             complement(31190..32707)
                     /gene="rpoN"
                     /locus_tag="PK589_08335"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNJ19648.1"
                     /translation="MKLRLDLRLSQKLIMTPQLQQAIKLLQLSRLELQQSLQQHLMEN
                     PLLDELVAETEESEETAATEREQPDTATTVAAETRGEGDDKPAESGENAEEFSASTWE
                     DYFDTDMRRGETEYSSSSKEEFPSYEQTVAKSTSLEDHLVWQLSLSGLSDREKAIGRL
                     IIGNLDDDGYLRMTLDELVAGSEYSVAEAESVLKDVQGFDPNGVAARDLSECLLLQLK
                     FLGRSQLGSLGSRPGVLKGSVLEAIVLHHLKDLEKKQYNRIAKALNISMDDVFEATRI
                     IEGLEPKPGRPFSNTQNYAIVPDVFVVKNEGEWEVLLNDDGLPRMRISPYYKQLMSSG
                     QSGSAETKAYLDDKLRAAQWVIRSIEQRNKTIVKVVSSIVKFQEGFFEHGIQHLKPLV
                     LKQVAEDIGMHESTISRVTANKYMYCPQGMLELKFFFNAGLQRADQPTDMLSSLTVRE
                     MIRQMVAAEDARKPLKDEEIAARLRTQQVLIARRTVAKYRAEDNIPSATQRRKFF"
     gene            complement(32726..33439)
                     /gene="lptB"
                     /locus_tag="PK589_08340"
     CDS             complement(32726..33439)
                     /gene="lptB"
                     /locus_tag="PK589_08340"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNJ19649.1"
                     /translation="MAASGLVKSFRGRKVVKGVSLDVQAGEVVGLLGPNGAGKTTIFD
                     MMVGLCQPDEGQIALSGNDITDLPMYKRARRGIGYLPQESSVFRRLSVEHNILAILEM
                     LGYSRSERMERVETLLKELDLVHIRKSKAYALSGGERRRLEITRALATSPLFMLLDEP
                     FAGIDPIAVADIQQIIIRLKQRHIGVLITDHNVRETLSITDRAYIINEGTILEAGPPG
                     VIEKSEMARAVYLGEGFRL"
     gene            complement(33511..34104)
                     /locus_tag="PK589_08345"
     CDS             complement(33511..34104)
                     /locus_tag="PK589_08345"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNJ19650.1"
                     /translation="MSCLLLSGEAVLSTHNAYAIETRGTKEPPASSAPTTITSRTMTV
                     NNQENTAIFDGAVVLTRGPLVVHSDHMVVSFHPSRNGADGKESGGPTKSKNLTNPTTA
                     ESKERDAAPAVSERSIRMVEATGRVNIKKEDGQATCQKAVYYEEQKKIVLTGEPVAWQ
                     KGTRVSGKRIIMFLDEDRSVVEGDSQVVIEGEGGGKR"
     gene            complement(34085..34657)
                     /gene="lptC"
                     /locus_tag="PK589_08350"
     CDS             complement(34085..34657)
                     /gene="lptC"
                     /locus_tag="PK589_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNJ19651.1"
                     /translation="MWERWVRRGLLALSVVLAAFLGYLLITRSNPSSSSRSVAPVEAE
                     SADAHIQDFTFTQTKGDLVQWKVQAEQARLYEKESRAILSNVQITMYGAQGKELSLSG
                     DEGTLDTQTKNFQLSNRSTPIVVETQSGYTIQTNHLVWTDARHEIQTPDHVTILGHGL
                     QVTGRGLLGKMDTEEFKILDDVHVDVVPAS"
     gene            complement(34947..35294)
                     /gene="rplQ"
                     /locus_tag="PK589_08355"
                     /pseudo
     CDS             complement(34947..35294)
                     /gene="rplQ"
                     /locus_tag="PK589_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(35313..36314)
                     /locus_tag="PK589_08360"
     CDS             complement(35313..36314)
                     /locus_tag="PK589_08360"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNJ19652.1"
                     /translation="MIKAMKDFQIPMRVEVDKDTLSPTFGRFTTEAFERGFGTTVGNS
                     LRRVLLSSLTGAAVTTVKIEGVLHEFSTIPGVTEDVTSIILNVKSLRLALQGDKPKTI
                     RLKKKGPGEAKGSDITHDGDVTILTPDLHIATLDKDAALDMEMTIKHGRGFVPAERNK
                     EEGLPIGVIAIDSIFSPIKRVNFHVENARVGRMTDYDKLTLEIWTDGTISPRDALSNA
                     AGILREHLDIFINPEERSDAKPSAGSEDLSDEVNKNLYRSVNELELSVRAANCLKNAN
                     IKTIADLVQKTEAEMLKTKNFGKKSLNEIKEILTEMGLSLGMKVDALPSGDAGVRSE"
     gene            complement(36399..37025)
                     /gene="rpsD"
                     /locus_tag="PK589_08365"
     CDS             complement(36399..37025)
                     /gene="rpsD"
                     /locus_tag="PK589_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNJ19653.1"
                     /translation="MAKYSGPVCRLCRREGEKLFLKGSRCMTEKCAIERRSYPPGQHG
                     QARQRTSDYSLQLREKQKLKRIYGLQECQFRGIFERAERQAGVTGDTLLRLLECRLDN
                     VVYRLGFGASRKEARQLVNHGHLMINGRKVKAPGALVKAGDSIEVRQKSRELIPIQGA
                     LAAVDGRGIPEWLELDRAAYKGTIRSLPTKEHIVLPVNEQMVVELYSR"
     gene            complement(37107..37490)
                     /gene="rpsK"
                     /locus_tag="PK589_08370"
     CDS             complement(37107..37490)
                     /gene="rpsK"
                     /locus_tag="PK589_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNJ19654.1"
                     /translation="MSVKKGKKKERKIVQSGVAHVQASFNNTIVTITDMSGNTVVWAS
                     SGNQGFKGSRKSTPFAAQRAGEAAARKAMENGMRQVDVYVNGPGAGRESAIRSLQSAG
                     LRINLIRDVTPIPHNGCRPPKRRRV"
     gene            complement(37532..37921)
                     /gene="rpsM"
                     /locus_tag="PK589_08375"
     CDS             complement(37532..37921)
                     /gene="rpsM"
                     /locus_tag="PK589_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNJ19655.1"
                     /translation="MARIAGVDLPKDKRIEIGLTYVFGIGRVAAQAILKKAGVDGAIR
                     VKDVSEDKIVKIREVIERDYRVEGDLRKEVSMNIKRLVDTGTFRGLRHRKGLPVRGQR
                     TKTNARTRKGRRSGVGSKPQKPAGSRA"
     gene            complement(38056..38169)
                     /gene="rpmJ"
                     /locus_tag="PK589_08380"
     CDS             complement(38056..38169)
                     /gene="rpmJ"
                     /locus_tag="PK589_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNJ19656.1"
                     /translation="MKVKSSVKPICAKCKVVRRRGVVRVLCANPRHKQRQG"
     gene            complement(38188..38406)
                     /gene="infA"
                     /locus_tag="PK589_08385"
     CDS             complement(38188..38406)
                     /gene="infA"
                     /locus_tag="PK589_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNJ19657.1"
                     /translation="MPKEDVIEVTGTVAETLPNAMFRVELENGHRILAHISGKMRMHF
                     IRILPGDKVTIQLSPYDLTRGRITYRFK"
     gene            complement(38428..39183)
                     /gene="map"
                     /locus_tag="PK589_08390"
     CDS             complement(38428..39183)
                     /gene="map"
                     /locus_tag="PK589_08390"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNJ19658.1"
                     /translation="MIVFKTPDEVTLMAQASRVVAEVLALLKGKVEPGITTEDLDRIA
                     EEAIRGRGAIPAFKGYRNYPKTLCASINEQVVHGIPSKRKLKEGDIIGLDLGAIVSGF
                     YGDSAVTVPVGSASEEALRLIQVTEEAMYQGIAKAVVGNRLSDVSHAIQTHVEQAGFA
                     VVTEFVGHGIGRQLHEEPQVPNYGKPGQGPRLQVGMVLAIEPMVNMGGSAVRILEDRW
                     TAVTQDGRWSAHFEHTIAIQPSGPAKILSQLSH"
     gene            complement(39180..39836)
                     /locus_tag="PK589_08395"
     CDS             complement(39180..39836)
                     /locus_tag="PK589_08395"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNJ19659.1"
                     /translation="MRLVFLGAPGVGKGTQADRVTAQFGWPKISTGDLLREAVRNQTA
                     LGIEAKKSMDAGKLVPDSVVIGMVRETLAAPSCQKGFVLDGFPRTVPQAEELQALLTG
                     RNLKLDRVINFRVSRHDVVTRLSGRRSCPKCQSVFHIAFAPPKIEGQCDRCGASLIQR
                     SDDKPETIEARLKVYDEQTAPLIAYYQAKNLLSDLDGSGEMAAVYTRLSALLLGLGAQ
                     "
     gene            complement(39846..41159)
                     /gene="secY"
                     /locus_tag="PK589_08400"
     CDS             complement(39846..41159)
                     /gene="secY"
                     /locus_tag="PK589_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNJ19660.1"
                     /translation="MFERLLTSFQNIFKIPELRTRVLFTLGMLIVYRVGAHIPTPGIN
                     GEALSEFLQKQGGALLGFLDIFSGGSLSRLTIFALGIMPYISASIILQLLTVVIPHLS
                     KLAKEGERGRKKIIQYTRFGTIVIALIQGFGIAVGLEQMNQGAFVLSPGWGFRFMTVI
                     TLCAGTGFLMWLGEQITERGIGNGISLIIFAGIVARLPAAVAQTFDLYKVGQLSFPLL
                     IVLAVVMFGVVAAIVFLESGRRKVPVQYAKRVIGRRVYGGQSTHIPLKINTAGVIPPI
                     FASSIIAFPATIAGFFETPWIKAIGSQLAPGSLLYTLMYVGLIVFFCFFYTAVVMNPV
                     DMADNLKKYGGFVPGIRPGQRTSDYIYSVLTKITFAGAIYLAIVCVIPEFLIYKLNMP
                     FYFGGTSLLIVIGVGLDTAQQIESHLLNRNYDGFLQKGKLRGRTS"
     gene            complement(41152..41601)
                     /gene="rplO"
                     /locus_tag="PK589_08405"
     CDS             complement(41152..41601)
                     /gene="rplO"
                     /locus_tag="PK589_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNJ19661.1"
                     /translation="MKLHDLAPSRGAKHKPKRIGRGPGSGHGKTATKGHKGLKARSGG
                     GKRPGFEGGQMPLIRRVPKYGFTNQFRTEYTIINLKSLAGLETTEAITPQLLMNEGLV
                     RRKGELIKILAQGELTKPLVVQAHKFSKSAEAKIQAAGGRAEVIPCV"
     gene            complement(41598..41807)
                     /gene="rpmD"
                     /locus_tag="PK589_08410"
     CDS             complement(41598..41807)
                     /gene="rpmD"
                     /locus_tag="PK589_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNJ19662.1"
                     /translation="MATEKKTTTAQASLRITLKRSPIGTPYKHRLVLRGLGLRKLNAT
                     VLRPATDQVKGMIAKVGYLLEVSPQ"
     gene            complement(41815..42306)
                     /gene="rpsE"
                     /locus_tag="PK589_08415"
     CDS             complement(41815..42306)
                     /gene="rpsE"
                     /locus_tag="PK589_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNJ19663.1"
                     /translation="MRVNPEELSLKDKVVFINRVAKVVKGGKRFNFCALVVVGDGQGY
                     VGVGKGKAAEVPVAISKAVEQAKKHLVRVPIKGGTIPHEVHGLFGAEHVLLKPAADGT
                     GIIAGGAVRAVVELAGAHNIIAKTLGRGNPFNAVRATLNGLQQLCDPQEIMRLRRAVG
                     SEA"
     gene            complement(42375..42740)
                     /gene="rplR"
                     /locus_tag="PK589_08420"
     CDS             complement(42375..42740)
                     /gene="rplR"
                     /locus_tag="PK589_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNJ19664.1"
                     /translation="MNTQEKNRKLARRKQRVRKSVVGTSDRPRLNVFRSRSHIYAQII
                     DDLRGHTVAAASTLDESLRKAVKSTGSIEGAKAVGKLIAERAKAANVSMVVFDRGGRQ
                     YHGRVKALADASREGGLQF"
     gene            complement(42750..43286)
                     /gene="rplF"
                     /locus_tag="PK589_08425"
     CDS             complement(42750..43286)
                     /gene="rplF"
                     /locus_tag="PK589_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNJ19665.1"
                     /translation="MSRIGRKPIAVPSGVDVKVAGRLVSVKGPMGKLDWKLTDGLSVA
                     VADGQLVVNRSGDAREIRAMHGLVRAELSNIIQGVTKGYEKSLEITGVGYKAQLQGRE
                     MSFNVGYINPVTYTVPQGIDVKVDKQTLISIKGIDKRLVGQVAANIRSIKPPDVYKQK
                     GIRYAGEVLRKKAGKTGK"
     gene            complement(43344..43739)
                     /gene="rpsH"
                     /locus_tag="PK589_08430"
     CDS             complement(43344..43739)
                     /gene="rpsH"
                     /locus_tag="PK589_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNJ19666.1"
                     /translation="MITDPIADLLVRLGNAARRRQDVVKVPVSKVKRQILNILGKEGF
                     ILGYGETQEDGHPIFSVQLRYVEEARPMITGMRRISKPGRRVYVGRDDVPKVRNGIGV
                     AVISTSKGLMTDSDSRRAGLGGEVLCAVW"
     gene            complement(43785..43970)
                     /locus_tag="PK589_08435"
     CDS             complement(43785..43970)
                     /locus_tag="PK589_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNJ19667.1"
                     /translation="MSRLALKNKAAVKSKFSCRDYHRCGLCGRVRGFLRRFRMCRICF
                     RSLSLKGEIPGVRKSSW"
     gene            complement(44032..44562)
                     /gene="rplE"
                     /locus_tag="PK589_08440"
     CDS             complement(44032..44562)
                     /gene="rplE"
                     /locus_tag="PK589_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNJ19668.1"
                     /translation="MRETYRDQVIPALMKEFGYGNLMQVPRLERIVLNVGMGEAIQNV
                     KLLESAASELGIITGQKPVTTRAKKAIAGFKLRQGMPIGAKVTLRSRRMWEFLDRLIS
                     LALPRIRDFRGISPKAFDGRGNYTLGLKEQLIFPEIEYDSVASIHGMDITIVTTARTN
                     DEGKALLKHLGMPFRA"
     gene            complement(44706..45032)
                     /gene="rplX"
                     /locus_tag="PK589_08445"
     CDS             complement(44706..45032)
                     /gene="rplX"
                     /locus_tag="PK589_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNJ19669.1"
                     /translation="MARIKTKIRKGDTVVVMTGRERGKSGKVLSVDTVTGKVLVEKLN
                     MIKRHTKPNQKLRQGGILEREAPLAISNVMFLCPVTKKPTRLGIKRQEDGRRTRLSKK
                     SKETVE"
     gene            complement(45059..45427)
                     /gene="rplN"
                     /locus_tag="PK589_08450"
     CDS             complement(45059..45427)
                     /gene="rplN"
                     /locus_tag="PK589_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNJ19670.1"
                     /translation="MIQNYTYMDVADNSGAKQVMCFHVLGGTRRRYGSLGDIVVVAVK
                     EAIPQAGVKKGDVSRAVIVRTTKEVRRDDGSYIKFDRNACVLINAQGEPVGTRIFGPV
                     ARELRWKKFMKIISLAPEVL"
     gene            complement(45489..45758)
                     /gene="rpsQ"
                     /locus_tag="PK589_08455"
     CDS             complement(45489..45758)
                     /gene="rpsQ"
                     /locus_tag="PK589_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNJ19671.1"
                     /translation="MKEGQQHRREWYGNVVSNKMNKTVVVAVERSVIHPIYKKVLRRV
                     TKLKAHDEGNVCKVGDRVQLSETRPISKDKHWRVVRVMVKGQPEK"
     gene            complement(45773..45994)
                     /gene="rpmC"
                     /locus_tag="PK589_08460"
     CDS             complement(45773..45994)
                     /gene="rpmC"
                     /locus_tag="PK589_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNJ19672.1"
                     /translation="MDVKDLSGLSLEELAAKEKQLRQELFNFRFQLGIGRLENPMQVR
                     STKRDIARLKTVRRQLELLQTQAGQSVGK"
     gene            complement(46023..46439)
                     /gene="rplP"
                     /locus_tag="PK589_08465"
     CDS             complement(46023..46439)
                     /gene="rplP"
                     /locus_tag="PK589_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNJ19673.1"
                     /translation="MLAPKKVKFRKMQKGRMRGKAYRGGAITLGEFGLKALEPGWITS
                     RQIEAARIAITRFVKRGGQVWTRIFPDKPITKKPAETRMGKGKGNPEYWVAVVKPGRI
                     MYEMGGVAPDVAKQALKLASYKLPIATKFVVRGEFQ"
     gene            complement(46467..47135)
                     /gene="rpsC"
                     /locus_tag="PK589_08470"
     CDS             complement(46467..47135)
                     /gene="rpsC"
                     /locus_tag="PK589_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNJ19674.1"
                     /translation="MGQKTHPIGYRIGYNYTWSSRWYADKDYARLLHQDIKIRKMVKA
                     KLYHAGVAKVEIERSGDQTRVIIHTARPGIIIGRKGAEVDKLKAALEKEYSGQAYITV
                     KEIKKPELDAQLVSENVATQLEKRVAFRRAMKRSVQSALRLGAQGIKIMVAGRLGGAE
                     IARTEWYREGRVPLHTLRAEVDYGFAEAHTTMGQIGVKTWIYKGELLPALQLKPDSTL
                     GRLG"
     gene            complement(47168..47521)
                     /gene="rplV"
                     /locus_tag="PK589_08475"
     CDS             complement(47168..47521)
                     /gene="rplV"
                     /locus_tag="PK589_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNJ19675.1"
                     /translation="MAEAKANLRFVRVTPRKARVVIDMIRGQQVPMALAMLRHTPKHA
                     ARVIEKLLRSAVANAEQKELGDSDEMWVSQAVVNCGPIYKRFRARSMGRANSIQKRTS
                     HITIAVAAPNAAHSS"
     gene            complement(47566..47853)
                     /gene="rpsS"
                     /locus_tag="PK589_08480"
     CDS             complement(47566..47853)
                     /gene="rpsS"
                     /locus_tag="PK589_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNJ19676.1"
                     /translation="MPRSITKGPFVDDHLIKKVEQMNQTKDRKLIKTWSRRSTVIPDM
                     IGHTFAVHNGKKFIPVFVTENMVGHKLGEFAPTRFFKGHGQAKTEKAVALK"
     gene            complement(47863..48690)
                     /gene="rplB"
                     /locus_tag="PK589_08485"
     CDS             complement(47863..48690)
                     /gene="rplB"
                     /locus_tag="PK589_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNJ19677.1"
                     /translation="MALKVYKPTTPGRRGMTAIQGEKLTKKKPEKSLTGFHLRTGGRN
                     NDGRLTIRFRGGGHKRLYRQIDFRRDKAGVPAKVAAIEYDPNRSSRIALLHYLDGEKR
                     YILAPVGLSVGDMVQSGEGAEVRPGNALPLVNMPLGTTIHNIELKPGKGGQLIRSAGG
                     SAQVMGRDGAYVQIRLKSGEMRKVLSTCMATVGQVGNVDHENVVVGKAGRTRWKGKRP
                     HVRGVVMNPVDHPHGGGEGKSGQGNPHPVSPWGTPAKGYKTRKNKSTDKFIIARRKK"
     gene            complement(48723..49016)
                     /locus_tag="PK589_08490"
     CDS             complement(48723..49016)
                     /locus_tag="PK589_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNJ19678.1"
                     /translation="MKGDLHQVLIQPLLTEKITALREQSNTVGFLVHPDANKIQIKQA
                     VESLLKVKVSRVNVVNIRGKIKRLGRFVGKRSDWKKAFVTLKEGEKLELYETV"
     gene            complement(49013..49636)
                     /gene="rplD"
                     /locus_tag="PK589_08495"
     CDS             complement(49013..49636)
                     /gene="rplD"
                     /locus_tag="PK589_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNJ19679.1"
                     /translation="MPIVDVVDSKKKKVGSVDLPDEVFGCKPHGSLVHEAVVMQRACD
                     RQGTASTLRRGEVSGSGKKPWKQKHTGRARAGSLRSPVWRHGGTVFGPKPRSYAFGMP
                     KKKYRAALQSVLSAKLSEGNVVVVSELSIAEAKTKLLANVLAQLGISGTALLVVADPN
                     SHVMQAGKNLSSVTVLRPEELNVYDVLRCHSLVIPQGELERVKEVWS"
     gene            complement(49640..50260)
                     /gene="rplC"
                     /locus_tag="PK589_08500"
     CDS             complement(49640..50260)
                     /gene="rplC"
                     /locus_tag="PK589_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNJ19680.1"
                     /translation="MTNGLLGKKLGMTQIYDESVLEPVTVIEAGPCRVVAIKTKERDG
                     YEAVQLSFGEVKESRLSGGELGHLKKHQAPPSRWLREFPKTGEVAVGATVKVDMFKKG
                     DWVDVVGVSKGKGFQGVVRRHNYAGGPETHGSMFHRAPGSIGASSYPSRVWKNKALPG
                     HMGDERVTVQRLKVIDARPDENLLFVRGAVPGGANGLLVVRKSKKS"
     gene            complement(50302..50613)
                     /gene="rpsJ"
                     /locus_tag="PK589_08505"
     CDS             complement(50302..50613)
                     /gene="rpsJ"
                     /locus_tag="PK589_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNJ19681.1"
                     /translation="MKVDQRIRIRLRGFDYRVLDQSVVEIVETVRRSGARVVGPIPLP
                     TRIEKFTVQRSTHVDKKSREQFEIRTHKRLLDIMEPTPETMDSLMKLNLAAGVDVEIK
                     L"
BASE COUNT        10896 a        15682 c        12919 g        11210 t
ORIGIN      
        1 gaccggtccg gcggtggccc cgccgggccg gtctcttttt cgagccgaga cgggttgcgg
       61 cgggagtggc cttccgccgc agccaccacc gcggacaaga gatcagacgt ttccgggtgg
      121 tagaagagcg atctgaacgt ctgatgcacc accgccgtat gccgctgccg atccgcgagg
      181 aactgttcca tcgccctctt gcgcgactcc ccgtagtagc ccattcgaat cgcacaacgc
      241 tgcagctctt catggctgtc aggcaacgca tgcgtctgca gatcgtgcat catctgcaac
      301 ttgtgttcga catcccgtaa aaaccaatag gcctcagtca gctgctgctg atcgtcttcg
      361 ttcacgaagc gatgccggca gaaacgatgc agagctccca cggtcttacg atccaggata
      421 tcgggcactg ctccaccaat cagcacctga atcgtctgta ccaggaattc gatctcccga
      481 atgcctccgg tccccagctt cacattgcga tgctgatgcc cgcgcaccgc gatcttctcg
      541 tcaatcattt ccttcactga acggacgtcg cgaatcaccg ccaaggcccg ttctcgcgta
      601 aggggctgga gatcagattg aaacacgaac ccttccgcca tctgcacaaa cgcctgcccg
      661 accgcaggcg aaccggcgac aggccaagcc tttagcagtg ccagtctttc ccagacctgg
      721 ccgcgctgcg catagtactt cgcataggcg tccaccgacc gcgccagttg ccccacggtt
      781 ccttcggcgc gaagccggag atccacgcga aacactgccc cttcacgagt ctgttccgcc
      841 agggctttcg tcagcgaccg cgccagcatt tcaaaatact cttcattgga cagaggtttg
      901 atgctcgcct ggcgattgcc ttttgccttg cgcgtttcac cgtctgcgga tgcatagaca
      961 taaatcaagt cgacatccga gctgtaattc aactcatgcg cgccaagctt acccatcccg
     1021 atcacgacaa attcggtttc tatccaggtg ccgtctggcc gttgatgcat cggggttccg
     1081 tgctgggttt tgagatcctt gtcgacgatc tcgtaggccg catggatcaa tactgacgcc
     1141 agatcagaca gcgatccgac cgtctcctcc acagtggcca ggcgcagaag atcgcgtacg
     1201 ccgatccgta gcatttcccg tcgccgcacg cgacgcagca cgtcgagctt caattcggtg
     1261 gccttgagag agccgagact ctggcgcaac gcggccacca tcccctcccg ggtcggagcc
     1321 gtcgtcaata catcttcttc cgccagccaa tagaccagca tcggatcgcg gatgagtgca
     1381 aacgccagag aatcactgtt tccgaagatg gtacacagca gccccaacat tctgggcgag
     1441 gcttgcagat attgcaggag agaagaacgg tttgcgcttc cagcaaaaag acgttcccaa
     1501 tgattcagcg cttggtcggg atcggcagta cgagccacct cagccagaag acgggagaga
     1561 atcccagcca acaggcggcg ggtatgcggc tcgcccgcca tgctttgaat attcgtatcc
     1621 gcctcggtgg gattccgtag tccatacggc cccaggatcc gcaccacctc gtccgcgtca
     1681 agaccggatg cgaccagcaa gggggtcgga tcggggaaca ccggcgaggc gcccgttcct
     1741 gcagggtcag cgagcgaatc attctgggag gaacgtcgtg ccatggcgcc gcattatact
     1801 ggcgcaattc ttccggtaca acgacagcac cttcgggtat actccacgga caccatgctg
     1861 cacgcgaaca ccgatcgcga ccagatcctc gccaccctgg cgccgctctg cccgggcatc
     1921 gagaccgaca tcctgcaaga ctttaccagc aggatggacg cggattactt cgcagcattc
     1981 tctcccaaac tgctcgccga gcatctctcg ttggccgcat tgctgacgcc ggaccatccc
     2041 tgcgaagtgc gattcgcaaa actcgatcgc aaacggttga ccatcaccat cgttgcgtac
     2101 gactactttt cggagttcgc caccatctgc ggcctcctct cggccttcgg tttgaatatc
     2161 gaggaaggcc gcatctttac ttctgccgaa agcgaaccgg cgcgaggcgg ccgccagaac
     2221 agtccctacg gcacacgtcc cagaccgcaa ggcaggccgg gactgacgag aaaaaagatt
     2281 gtggacgtct tcacggtcac gcctacggag ggagagccct ttactgctga cgatcaaaag
     2341 cgcttggccg aacaattgtc ccgcatgatc attctgctgg acaccaacga attcgatgaa
     2401 gcacgccagc aggtcaacag gcagctgatc gaatacttgg gcacacggcg tagttcattc
     2461 agcggcctgc tccataccgt tcacattacc ttcgacaaca gccagtcggc caccgacacg
     2521 atcatggaca tccggtccga cgacacgccg gcgtttctct atgccttcgc caatgcgctg
     2581 gctatgcgta acgtctacat cagcaaggcc cagttcgcga tcgaagaggg aaagttgcac
     2641 gaccgctttt atgttcgaaa tcgcttcggg caaaagttgc ttgatccggg tgatcaagag
     2701 caactccggc tcactgcggt gctcatcaaa caattcacac acgcgctcac gtgggcaccc
     2761 gatcccgcta aagcgctgga atcgtttgat cagttcctcg accttgtgct tgaggggtcc
     2821 cgccaagccg gaaaaaaaca ggcgtgggac tttatcaatg ataaaaagac gtttccactg
     2881 ctcgcgcggc ttctcggcgc cagtgacttt ttatgggaag actttcttcg gcggcaacat
     2941 gtcaacctgc tgccgctgct caaggactat cgcgacgcgc cgctcatcaa gcctcaagcc
     3001 acgctgcgga aagagctgaa ccgcctcatt gccaaagcca agaccgacga agcacgcaaa
     3061 gaagcgctca accgcttcaa ggatcaggaa ttgttccgta tcgacatgaa acacatcgtc
     3121 gagccgggaa ccaaccttcc tgacttctct ttggccattt ccgaactcgc cgaagtcatc
     3181 gtcgagcgca gcttgctcga ctgccaagca aagctcacta aacgctatgg atcgcctcga
     3241 ctcgccaata agaaaccctg cccgtttgcc atcctgggtg caggaaaatt cggcggccgc
     3301 gaactcggat atgcctccga catcgaggtg ttgttcgtct atggcgggcc tggtcacacg
     3361 accgggaaag acggcatcga aaacagcgag tatttcgagc gcctggcaca ggaactgttg
     3421 caatggatag aagccaagca agaaggcatc ttccatatcg atgtccgcct ccgaccacac
     3481 ggaggcaagg ggtctctctc caatgcgttt gatgaggtct gtaaatacta tagcgccgac
     3541 ggcttagcgg cgccgttcga gcggcaggcg ctcatcaagc tgcgtcatat cgcaggagac
     3601 gccgcgctcg gcaggaaggt ggaagcccat cgcgacagtt ttgtgtatag cggcgctccc
     3661 tgggatctga cgactgcgat cgatctgcgt cgccaacaag tcaaacaact cgtcgagccg
     3721 ggccaggtca atctcaaaca cagccacgga ggcattgtca cgctggaata tgcgatccaa
     3781 tacctccagg tcatgcatgg tcatcgccat gcgagccttc gtacgcctaa cacgctgcga
     3841 gcgttggccg ccttgatcga cgcggacctg attccacgta caaccggtga gaatttgcgt
     3901 aaatcctacc tcttcatccg catgctgatc gacggcctgc gcatggtgcg cggcaacacc
     3961 aaggatctcg ttctgccgcc tccggactcc gacgaattca tcttcctcgc ccgccgcgtc
     4021 ggatatcaga cagaagactg gcaagccggg gcgaaacacc tgctgtcgga catcgaacag
     4081 cacatgaaac agaatcgaga gttcttcgag aagatgttcg gaaaggtgtg aggccacgca
     4141 ggccactgaa gtcattgccg gctccatcgt cttcgtaaca ttgagccccg ccctatctgc
     4201 tggaagggcc gttccacaag cacctctcgt cctctgcttc tcgttaccta cgtagcgcat
     4261 gacccccgcg tcgagttttg gccaaaccat ggtacagtgg aacggcggag tcacttcggg
     4321 aggaggatta ccatgttgac gcgagtattg gcagggctgt tattgagcgt ggtgctggct
     4381 tcatgcgggc aaggcgacgg cggagccggc tccggcatca agccggttgg agaatgggcc
     4441 aagatcggcg agacggatac ctacatctat tacgccgatt acgccagcat caaaaaagca
     4501 gatgaaacag tcgtcatgct ggacctgttc gactacaaaa gcgcacagag cgaaggggga
     4561 ggagccccag ccctgtcaaa ggccaccgag cgcgaatacg actgtcaaaa taagaagagc
     4621 cagccgctca agtccagctg gtattccgaa gggatgggag ccggaaccgt cgtgcgctcc
     4681 accggcacct cgaatcaatg gtcctctgct gctcaaggga ctgcaaccgg agggctgttg
     4741 aaggccgcct gcggaaatta gccgacggcc gaaacacgcc gcctcgcgct acgttactcg
     4801 agccggcagg caatacctgc catccggctg ccgctattct acgcaggaga cgtacgcctt
     4861 gggacagacc gcatcaagcc ccccggacgg attcccctgc aagggaatac gctgcatctg
     4921 ttccagcacc tgctggaaat gccccggtgc gcgcgtccag aacaggtagt tggcccgcaa
     4981 tcgatcccgg ccgaacgcca gcagttcggc aacggttggt actcgcccct ttccgtcatg
     5041 cctggtgtta tcgtagttcg cctgcatgac cgagggcgtc aggggaatca ggccagacat
     5101 ctgcgggtaa taggaataga tccccttggg cttgttcgga tgatcgaagt tcaatcccgg
     5161 atcatctaga aaactatccg ggccgcccaa cccggtaccg atcgtgcgca tgtgctcgat
     5221 gaagccgggc aatatctcac gcggatagtt cacaaactgg taggtcacgg tattgggaaa
     5281 agcggcacgc atctgccgct gcacatccag caggttgtcg taaaactttt cggtgtcgtg
     5341 gctggacaga agctcgatcg gctgccccaa ggcagtctcc gggagaccga tgccttcgaa
     5401 gttcgcatgg ccgttgaaac gcttgcccag ttcggtcagc agcgccacca gtcgatcgtg
     5461 cacacccgag ttccagagtt tgaggttgta cccgcgagga atgctctgcc cataggtgct
     5521 gaagggaaag gcgccccctt cgtaggcttc cgttcgcata taggtcggca tcggcaggat
     5581 ttgcggattg aaagacttca gttcgagaag aatgaccaac tgtttgttca agggcgccaa
     5641 ttcggccagg accttttcga tccctttgaa gtcgtagcgg ccttcttccg gttctagttc
     5701 cggccaggca aaccgcactt ggagaccgcg tagcgcaggc gtcgccttca gctcggcgta
     5761 gatctgcgtc atgacattcg cattcgcccg cataaaggtc atcggcgcat aataatgccc
     5821 cggatgccac ttcacgatgc cgggggtggt tggcacctcg gcggcatcca ctgcgcccca
     5881 cgcccatggc aggcccgcga gaacgaggaa gctcaggata aacatcagag ggagagatgt
     5941 ttttccggca gggatagaga ggggccattt acgtactgac cgtccattcg ctagctcgtg
     6001 catacgtctc ctttccgata ggtctactgc cagtgaaaga ccgccgcgcc tctcactgca
     6061 ggcgcatgat agcacgagag gctcccaccg tactgtcctg tggggttcag tacttatgta
     6121 gtggaggcaa tgaggaaaat cggccccctc tcctccattg tcgagcgagc cggcactcga
     6181 catgaattag gtggttcgtg agtcagagaa aggtgacatc tcaggaaggg cgccaaacgg
     6241 cggtcatggc gaaactgttc gagcacggcg caaaccggca gcgctcgtgc gccgccggtt
     6301 tggcatagaa aaccttctcc ttacaactcc agcatttttt gataggcccg aagaaggtcg
     6361 ccaacagaca acaccccgat gatggtgccg tcttctgtca ccggcagatg ccggatgcct
     6421 tcttttttca tcagtcgcaa cgcttctttg atcggttcac tatcttcaat ggtcaccaca
     6481 gccttgctca tgcatgacat gacggtggtg ctgttcggat ccaacccctt ggcgacggcc
     6541 ttccggctca gatccgtgtc cgtgatgatg ccgagatagc gtgatccgtc atcaacgagc
     6601 agtgacccga tacgccactt ctgcagcaac cggcctgcct ccttgatcga agcgcctttg
     6661 tgcacgctcc tgacctcaga cgacatgaac tcggccacgg tgcggccatc gatccgctca
     6721 ccgagcgtcc gtctccaatc tcggccgacc ctggtgcttt ttttgagctc aagctgcgcg
     6781 aggcagctct cgaggacctg cttacgttga tgcaggctct cacgctcgag attatgcgtc
     6841 ccggcctctt gcgcttcctc cggaagaatt gccgcttcac cgagcttggc atagtaatac
     6901 gcttcgagct catcggacgc gccgccgaag gtttcactga gcacctcttc ggcctgctcg
     6961 tcaaaacctt ctagtgatgc cgtggccttc cgtcgcgata aaaatggctg gaacgcggcc
     7021 aacgtctcac gtatttcttc tatatgccgg ctgagcagat cagccggttc ctgcgatgat
     7081 ttcgttctct tggccatgct acctccttcg tgaaggcaag agaataaccc aactcccata
     7141 tcggctcaag tctcacgggc taggccggaa acggttgcgg cctcaggccg gagatcgccc
     7201 catccgcagc attggccgaa gaggctgact tcgccgaggc agtcgatttt gccacggcct
     7261 tgccccgcac acggtgcatc ttatcgcgtg tatgatatgg catcaggttt tcgaggaccg
     7321 ccgggagttt gtcgcacggc acatcttcca tgatcttcgt cgccagcttc gggtccggac
     7381 ctgaacgccc gccgacaaag atatcgacgg cgtccaccac cttgccgccc actcgggctt
     7441 ttttgccaag cagtccgata tccgcgacca ggtgattgcc acacccggca gggcagccgg
     7501 accaatgcag cgtgatcggc ttcagccctt ccagcttgcg ctccaggatg tgcgcggttt
     7561 ccacggcccg ccgcttggtt tcgatgacgg ccatattgca gtagtcgctc cccacgcagc
     7621 tgaccagccc cttgtacagg ggagatgggt tataggcgaa ttgctttacc agcggctcct
     7681 ccgccagttc acccaccaca cggtcgctga tgtgcgggat cacgatagct tgcgcaggcg
     7741 acagccgcac ctcacccgtt ccataccgct ctgccagcgc tgcgatgttg cgcaaatcac
     7801 ccgctttgat ccggcccacc aatatcttga gaccgatata gttcatgccg cgttgttttt
     7861 gacggaaaat gccgatgtgg tcgcgctcac ccgccgccct ggcatcgacc cctgcagacg
     7921 gcaacgcgcg ccccatgcgc acttccacct cgtgccgaag ccgggactcg ccccaggctt
     7981 cgaccagaaa cgcgaggcgc gcttgggtgc gattttcacg cgggccatga tcacggtaga
     8041 tgtgcagcaa cgtccggcag acatcgaatg cttcggccgg attcacgaac atatccaacg
     8101 aactcgcgat gcgatatccg ccggatccca atttgccccc gaccaacacg ttgtagccga
     8161 aacatttgtc gtgccctaaa tcatgatacg caggcaccag cgcaatatct tgcgtctccg
     8221 tatgcagaca attgtccgtg cagccggtga cggcgatgtt gcacttgcgc ggcaaattcg
     8281 tataggccgg attgcccaac acttcgtggt tgatagcgcg gacgatctcc gtcccatcca
     8341 gtaattcatt cggattcaat cccgccaccg gacaggtcat cacgttgcgc acactgtcca
     8401 tgccggtttg catcgacgta agcccgacct catccagctt cgcgaacacc gccggcacat
     8461 gcgcgatcgt caggtgacgg agttgcactt gttgccgcgt ggtcacatcc atcacgccat
     8521 tgccatacgc caacgcgata tcggccagcg cttgaagttg aaccgatgtg gtttgccctc
     8581 ccggcatccg tactcgcaac atgaagaagc ccggcgtggg attccgcaga aacagcccgt
     8641 accatttcag ccgttgaata tcgtcttcgg gaatggactc ccaccccgcg tgcgcatagt
     8701 gctcaagcat gtcgcgtacc gctaatccgt cacgctcggt tttgatggct tctatcttgt
     8761 tcatatcgtg ctccccagtg cgacggaata gtccaatccc gattttctca acacgacagt
     8821 accctctcga ctcaatcgat ccacgacgag caagacctgc gaggcactca gaccggagag
     8881 ggccatcaat tgctcgaatg attggggagc ctgccgccgg agaagcgtca gcactacggt
     8941 ttcttcattc gtcttcatga tgtcctcctt cgtctgagtg ccgcgtcacg gcatgttggt
     9001 cactgactta gaacgtctta tactcgagcc acttgccgtt cagcgtaatc ttgcaccggg
     9061 cttctccctg gctgccggtg actttttcca tatccagcgt aaaatcgatc gcactcatga
     9121 tgccgtcgcc gaacatttca ttggccatct cacgcagcga atcgccgtag acaccgatga
     9181 cctccatcag gcgatatttg aacggatccg tcaggttcgg gaaatccgcg cgtatcggaa
     9241 aggcgctcaa ggccgcgacc tgttcctgat tcagacccaa cagcgccccg accttcgcgg
     9301 cgtcggccgg aaccaggcga tgattgccgt tcagggctgc cgccacaaag gtcggatttt
     9361 tgccgacctt tttcgccact tgggcaatgg tcactttctt ttttaatcgt tgcgccttga
     9421 tcgccttccg cattgcttcc ttctccatca tctcctcctg tgattgatcc gcctgcaatg
     9481 atgcatactc gccgctctaa cttacgaccc gaccaccgcc gatgtggcgg gactcgatac
     9541 cggagtcacc gggacttcgg ccacccggta gggactcacc atccagtaca acccgccgac
     9601 aaacaatcca ccacccacga ggttccccag cacgacaaag agttggttgt accaaaaggc
     9661 cgcccacgtc acggtgacgt catgcggtag gaacagcgcc atacccaaca aggattgatt
     9721 cgcgatgcta tgctcgaacc ccgtgccgat aaaggcaaac aggcaccaga agatgagcag
     9781 gatcttggcg gtgtcattcg tcgtccgccc ggtcatccat acggccaggc agatcagcca
     9841 attgcaaaga atcccccgca cgaacaactc ccacgcggga agagacattt tcgccgccgc
     9901 cactttttca atcaggtccg ccgacggccc cttcgcgaac acgccggatt cgacaatcaa
     9961 ccaggccaac cccatcgatc cggccaaatt tccgaaccag gaccaggcat tgagctgaat
    10021 gacttgcgtc caggataggc tgcgtgacag accgccgatg gccccgatca agttgttgcc
    10081 ggtaaacagc tcggatcccg caaaaatcac aagcgtcaat gcgataccga atgacacacc
    10141 catgactagt ttcaccaccg gtgatccggc ggccgccaac ggccctccga gactgaaaat
    10201 caacgcaatg ccgaacccga gataaatccc ggccaaggac gacaggatga aatacccgcc
    10261 cggcgcttgt tccaaaaaca tccacttctt tgccgccacc cccgcgatcc gctcatgatc
    10321 cgctgtgtgc atgaggctct cccttcgatg gtccatcagt ctgaacagct caaaaaaaaa
    10381 gacgccctca tcccctgtgg gggagaagga cgtctttgtc ctgttcaacg agggtgcgat
    10441 tccctcgtca gttatgctgc gccgacttca acgtcggcag aaacgaaaaa ggcgtccgcc
    10501 actcttgaaa gaatgacgga cgccattgtc cgtgtaccgt atgtggtgca ggttcgaaat
    10561 caggcgtcgt tgcctgctct cacccacgca ctgcatgtat accatcgcct cccggtttcg
    10621 tcaagtgctc tcgacgagga ggccgccggc tacacgtcgt aatacagaaa gaactcgtac
    10681 ggatgcggcc gtaaccgcat ggtatcgact tccttggtcc gcttgtaccc gatccaggcc
    10741 tcgatgaggt cctcgctgaa cacgcccccc ttgaggagga attgatggtc cttctcgagg
    10801 tggttcagcg cctcgtccag gctgcccggc atggtgcgaa ttttcgccgc ttccttggcc
    10861 tcgagatcgt acagatcctt ttccgccggc tctcccggat tgatcttgtt ctcgatacca
    10921 tccagaccgg ccatcagcat ggccgcaaag gccagatagg ggttggccgc ggggtccgga
    10981 aagcgcactt caatccgctt cgccttgggg ctcggggaat acatcggaat acgaataccg
    11041 gccgaacgat tgcggctcga atacgccaac aagaccggcg cttcaaagcc cggggtcaac
    11101 cgcttgtacg agttggtcga cgggttggtg aaggccgcca gtgccggcgc atgcttcaga
    11161 ataccgccga tgtagttcag acacatctgc gacacgccgg catattcctt cccggcaaag
    11221 agaggcttgc cgtccttcca gatgctctgg tgcgtatgca tgcccgaccc gttgtcgccg
    11281 aaaatcggct tgggcatgaa ggtcaccgtc ttcccatggc gacgcgcgac gttcttgacg
    11341 atgtacttga acatcatcat tttgtctgcc gtccgcaaca ggctatcgaa gcgaatatcg
    11401 atttccgcct ggcccgccgt cgccacttcg tggtgatgct tctccgtcgc gataccggcc
    11461 ttctccatct ccaaaatcat ttccgtgcgg atgtcctgct gggtatccgt cggcggaaca
    11521 gggaaatacc cttccttgtg acggatctta ccgcccaggt tgaccccgtc ctggcccatg
    11581 ttccatacgc cttcatcgga atcgatgtgg tagtaggcgc tgtggttcgt ctggtcgtag
    11641 cgggcatggt cgaaaataaa gaactcggct tccggccccc agtaggaggt gtcgccgatc
    11701 ttggtgctct ggagatattt ttccgccttc tgggcgatga accgaggatc gcggtcatac
    11761 atttcgcgcg tgatcggatc gaccacattc ccgatgaggc tcagtgtggg cacggagcaa
    11821 aagggatcca tgcaggcggt tgccgggtcc ggcacgagca gcatgtcgct gttgttgatc
    11881 gctctccatc cgcgaatcga tgaaccatcg agcccggatc cgtccttgaa caggccttcg
    11941 gtcaactcac tgacaggaat cgtaaaatgc tgccaggtgc cgatcaggtc gacgaacttc
    12001 atgtccacga cctggacctt attcttcttt gcaaaatcta acacctcacg aacgttcatc
    12061 ccctcctcct ttcaaccgcc gataaaagaa ctacccacac cctgacgttt acgccgcatg
    12121 agctcccaaa aacaattcaa tctcgttgag aatcgacgcc cgaatcgttt cgagacgccc
    12181 ggcgtcttcc accttcttcc catccatccc cgtcacgtaa aacacgtcgg ccacttgatc
    12241 gagccgggtg gaaatccggg acgcatgcac cgacaagccc aattgaaaaa tagcgttcgt
    12301 gataatgtac agcaatcctt gccgatcatc cgcaaaaaca tcaagaatcg taaaccggtc
    12361 ggacgtctca ttatcgatac gcacttccgc cggttgacga tgggcgggca atgaccggcc
    12421 cagattcaat cgcgccccgc gccgcatcac cgcatcgatc gactgacggc cggtcagcac
    12481 ctccgtaatg gtcgcgccga tcgactcgca ccgatccgcc ggcggagcgc cctgataatc
    12541 aggatcggcg acctggaagg tatcgacgac cacgccgtct tttctggtca cgatctgcgc
    12601 gtccagaatc tgcaagccgc cagccgccat caccccggca atcttcgaaa acaatccggg
    12661 aatcagatca ttatgtgcaa tcacggcata ttcgcatgtt cccaacggag cattgaactc
    12721 cgtctccacg accacgccgc cctcctgcag acggcggatg gcgcccaagt gggcggcaat
    12781 gcgcagcgga ctcgtaccgt agacataccg caggggaaac tgttcgagtt cggaacagat
    12841 ccaggttttg tcggctggaa ggttgtgatc cccctgcgac tgtcccgcca cgtcatcggc
    12901 gatacgcgcc aagcgctgcg gctcatcggc cgtctcccgc tcgcccgata cttcctgcat
    12961 ggctcgcagg tacagttcaa ccagcagcga ttctttccat ttcgtcagca catcagggcc
    13021 cacggcagcg atgtcggcag cggtcagggc cagtaacttg cgcaacactt ccggcgtgcc
    13081 gacttcacgc gcaaacggca acagcacctt ttcatcatac gggtcgcggc gaaaggccgt
    13141 gtgagccatc aacagatgcc gatggaccag catcaccaag gtacgcgtgt ccggctcatc
    13201 gaagccgaga cgcgcggccg tttcttccgc caaccgcttt ccaacctcac tatgatcttc
    13261 ctcgtgacct tttcccaggt catgaaggag gagcgccaga tgcagcagat ccttccgttt
    13321 gatctctcga tagacctcac cgaggacccc gctatggtcg gccagagttt cggctttcgc
    13381 caccgcgagt aaactatgct cgtcgaccgt gtacttgtgg tactgattga attgcatcag
    13441 tccacgcaca cgtgagaacg ccggcacgag tttctccaac agatgcgccc gatgcatggc
    13501 ttccagggtc cgcgccgtgc ctgggcccgc cagaatcgaa aggaaggttt ggcctgcctc
    13561 cggcgtgtgg aactgttcga cggacagcgt ctccgcatgc cggtgaatgt cttccaggag
    13621 agacggatcg atactcaact gccgcgaacg agccaggttg aagagcgtaa gcagcaatgt
    13681 cggctgcacc aggacctgtg aacgcaactc aaccggaacg gtcaacgcct caccatcgac
    13741 ggaaaaatat tgttggatgc gcggcgacgg gagccatcga acgagccgtt gccacaagga
    13801 gcggcgtcgg caccgttcca caaatcgcat caaggcaacg tgcagcccca tcgtatgccg
    13861 atagtattgc tgcatgaatt gttcgacggc gagcaaatgc ggacggtcgg tgaacccgag
    13921 atgtttcgcc agccagacct gttcgtcgaa ggtgagaatt tcctgcgcca tgccggctcg
    13981 aatatggaga aaggagcgga cccgcaagag aaactctctg gcttccttga tcgagcgata
    14041 atctgattgg gagagaatcc cccggtcaga gagttcgcga atggtgggcg cctgaaatcg
    14101 cgcggaaccg atccattgca ggaggtgcag gtcgcgcaag ccccctttgc ttttcttgac
    14161 gttcggctcc agcaggtaca ccgtctcccc gaacttctgg tattcacgct gccgttcggc
    14221 gaccttctgt tcgagatacc cgtcgaaacc gcttgaaacc actttgcgca cgtagcgcgc
    14281 atggaattgt tgaaagagct cggggcttcc cgtcaaaaac cgggcctcca tcatggaggt
    14341 gcgcacagtc agatccgcta atcccaaatc gatacagtcc tgaatggtgc ggacgctgtg
    14401 cccgacctga aatccgatat cccagagcgg atgcagcacc tgcttcacca gttcaggaat
    14461 gaccttggcc gcatcctgat gaaagaggaa catcaaatcg atatcggagt gcggcgccag
    14521 ttcccgccgg ccgtagcccc cgatcgccac caggcagcag tgctggaatc ctgcggtcgt
    14581 gagcgcctcg ccgccggtcc tggccgcggt ccggtaccgg ccgatgatca agccatcgac
    14641 cagatccgtt gtggccgcaa ccacttcgtc gccagacgca ccggccagta accgctgctg
    14701 aatcgcctgg cgctgttcag ccagcagttg agacaccgga atggcagctg cgtcctgcac
    14761 catatgaaac cccgcgtcca acgcgtgcga catactcaga gcgcgctttc tcccgtttca
    14821 ccggtccgga tcctgacggc cgacgcgagg tcccgcacaa atattttccc gtcgccgatg
    14881 ctgcccgtct ttgcggcgcg cgtgatggtc tccaacaccc gctggacctg accatcattc
    14941 accgccactt cgatcttcac cttcggcacg aactcgatgg tgtactcctg gccgcgatag
    15001 gtttccttgt gccccttctg ccgcccaaag cccttgacct ctgtgacggt catgccctga
    15061 atcccgatct cgagcagggc atccttcact tcatcgagct tgaacggttt gacgatggct
    15121 tcgattaatt tcatcctggc ctcctgagac cgccgaccgc ccacagtcga tggcgtcgta
    15181 tcacgaatag gcgcgttcgt tatgttgact caaatcgaga ccggtcgctt cctcttcggg
    15241 cgagacccgc agtcccgtca tcgcatcgac caatttgagg atggcaaagg taccgaccag
    15301 tgagaagacc gtcgtgacca ccacgaccag gagttggacg ccgaacaacc cgggattgcc
    15361 gaagaacagt ccgtcggcgc ctccgggatt gacgactttg ctggcgcaga gacctgtcgc
    15421 cagcactccg atcacccccc cgactccgtg gatccccacg acgtcaagcg agtcatcata
    15481 gccgaatcgc cccttccaga caatcgccgc gtagcaggac atcccggcaa ccaggccgat
    15541 gaggatggcc cacagggggc tgatatatcc cgctgcaggc gtgactgtag ccaggccggc
    15601 caccgcgccg ctggccaatc ccagcaccgt gggtttcccg cgatgcatcc attccgcgcc
    15661 gcaccaggag atggctccca tacaggccgc cgcatgtgac gcaatgatgg ccgacaccgc
    15721 cacgccattc gcccccagtg cgctcccccc gttgaaccca aaccagccga accacagaaa
    15781 tccggtcccg agcagggtca tcggcaaatt gtggggagcc atataatccg tgccatagcc
    15841 ccgccgcttg cccagcacga tcgcacaaat caatgcagcc gcgccggagc tgatgtggat
    15901 gaccgcaccg ccggcaaaat ccaacgcccc cagttgagcg agccatccgc cgccccagat
    15961 ccaatgggcc agcggcacat acacgagcac cgaccacatg gcggcaaaga gcacaaccga
    16021 ggtaaaccgt ttgcgttcgg caaacgcgcc ggtgatcagc gccggcgcaa tggcggcaaa
    16081 catcaattgg aacagcataa aggcctggtg cggaatcgtc ggggcataga ccgcgtgcgg
    16141 ctgactcccc actccgctca ggccgatcca atccagcccc ccgatgaccc cgcctttatc
    16201 cggtccgaag gccaggctat atccgaagag gatccacaac aggctgacca cgctcaggat
    16261 catgatgctc tgcatgatcg tccccagcac attcttggtg cgcaccagcc cgccgtaaaa
    16321 caaggcgagc cccggcatca gcatcgccag cacgaaggcc gacgagacca aaacccatgc
    16381 cgtgtccccg ctgtcggctt tgagcggcgc attctccgcc cacgcctgtt ccgcccccaa
    16441 tccaccaatc agggtgagtg cccctgccag gcccacgagg aacgttttcc actgtgccga
    16501 tgccttcacg ctgtcctcct tcggctacgt tgcctgccaa gaggcctcct ccttcaccat
    16561 gctcaggagg aggcccgggc agaagcttag aacgtcttcg tgaacttcag cttataaaaa
    16621 tcctgattgt ctgtcagatc ttttccgacc gtgaaccgga aggccatgtt tcccggcaac
    16681 ttcatttcaa cgtacgggcc gatccaacga tagtaatcct gagccgcccc cggcgcgctg
    16741 ctatcgcgcg tcgtcagcaa atgctcatag tgcgcgccca tcgaccacat gctgttcacc
    16801 cgcacgcccg cgctcaccca gtagtgcaga aagtcccagg ttccccggcc accgccctga
    16861 atcgggccca gacagttcgt gcccccggga ttgccgcagc cgatcgctcc gccgccgccg
    16921 ctgccgccct cgctgcggat cgccttgtag tagcccatgt agaactcgcc ttcgaaacga
    16981 ttgtcgatgt agttcgcaat gatgttcggc accgcgcctt caaaggccgt ggtgcgctca
    17041 ttgacgctgc cgccgccgtt cggaaagaac ccgccacggg acgacaacag gctgccatgc
    17101 acaaagccga tcatcggcgt gacagacaag cgtttttcca ggaacgtgaa attgagtccc
    17161 acgtccgttt caagccaggg cgcgttgtca tgcaccccga cgccgctgat catggtccaa
    17221 taggtgaagt taaaggcgag ggcgatgtta tcggtcagac cgtaggtgcc gtagatggat
    17281 ggattgaatc cgaagaagct gtcggcctgc atcgccatcg tcaccgacac ggggtgtttg
    17341 gtggtatcca tcaagtcgtc cgtcgtcgcg gcctgcgcct cgaccagcgc gcctcccccg
    17401 ccgcctgcca tcaatgccaa tcccaacacc catgcccgtg cctttcgccc aaccaacata
    17461 ttcatgacgc ctccaaagag agaagaagtg aaagtggccc gctccgagac catcgtgccc
    17521 gacaatctgg agttccgtct ctctggcgcc attgccagtg ttgctgaccg acgtcgttgt
    17581 cggtcgggat gctgcttgtc taccgatacc cgttggtgat cggcatccgc cgatccttgc
    17641 cgaacgccct atgcgtaatc ttgatgccga gcggcgcttg cctgcgtttg tactcgctgc
    17701 gatccaccat tatcatgacg cgtgccaccg tctcccgctc gaaccccatg gccacgatgt
    17761 cttcgagggc ccggtcctcc tcgacatagg ctttcagaat ggggtccaac accgaatagg
    17821 gcgggagact gtcttcatct ttttgatcgg ggcgcaactc tgcggtcgga ggccgttcca
    17881 ggacacgttt tggaatgacc ggttccagac cgttcagatt ccgcagttgt gacagttcgt
    17941 acaccatggt cttgggcacg tctttgatca ccgcaaatcc gccggccatg tcgccgtata
    18001 acgtggcgta gccgacgctc atttcgctct tgtttccggt ggtcaacacc aggtgtccga
    18061 atttgttgga gtaggccatg agcagattgc ctctgatcct ggcctgcaag ttttcctcag
    18121 ttgtatcggg acggtgtccg gagaatggct tggacagcgc acgcaaataa ctcttgaacg
    18181 tcgtcgtgat cgagagagta tccacctgga tgcgcagacg ttgggcgaga tcggccacat
    18241 cctctcgact ggctcgcgag gtgtaaggcg agggcatgaa gaccccgagg acattatccg
    18301 cgccgaccgc atcgaccgcg atcacggccg tgagggcgga atcaataccc ccgctcagcc
    18361 cgatgacgac ccgcttgaac ccgttctttt ggacataatc ccgcacgccc aggaccagcg
    18421 cgcgataggc ctcctccagc cgatccaatg gcgattccat ggccggcacg gcgattgacg
    18481 acgatttcct cgccggcacc cgcaccgcaa cacgatccac cagcgcggcc actcgcggcg
    18541 gcaagggcct ggcgcgccgt tcaccctgtc gcgcgcgccc caccgccgcc acatcgagat
    18601 ccgcaatcaa aagatcctgc tcaaaggact tacctcgcgc caccacctcg ccggtgtgat
    18661 cgacgatcac gctacggccg tcgaagacca attcatcctg cccacccacg gtgttggtat
    18721 aggtgaccat cacgcgattc tcgcgggcac gggtagccag cacctgttcg cgcagtcggc
    18781 tcttgccgac atggaacggc gaagcgttga tgttcacgat gacctcagcc cctgccgccg
    18841 cctgcgcgcg tgtcggaccc tcgggaaacc agatgtcctc gcaaatgttc acgccgatgg
    18901 tggtgccgtt cagcaccagc agtggcagac gcgtgccggg ccggaaatac cggctctcat
    18961 caaagacgcc atagttcggc aacagccgct tgcaatagtt ggcgaccagc cgtcgctcac
    19021 atagcacagc tgccgcattg tagagatcat gccggccggc cggggaaacc gcctctgcct
    19081 ccgacggtgt cacgccggtt gcctcgcgcc cgacataccc caccaccacc actaatccgt
    19141 gcgcttccgc cgccaccgcc tttaacgcac gctgcgtgtc ttcgatgaag cggggcttga
    19201 acaataaatc ctccggcgga taaccggtga tggccaattc aggaaacgcc acaatgtcag
    19261 ccttggcccg acgcgcgtca ttgatccagg tcttgatcag cgccgtattc ccgacgatat
    19321 cgccaaccgt cggattgatc tgagccatgg ccactcgcag gacgcgcatc atcagctttc
    19381 ccctgcactc gcactttgct caaatcggtc attcaacgag gccgcaagga gctcaggcac
    19441 cgaggcgtac tgtgagccgt acgtcgaggt ggctgatcga ctgagaacga agttgagggc
    19501 cgatttcagc atagtgctca aaaaaaaagt cctcggaccc accgtatctc ggtggaccag
    19561 aggacgtcgt tgtcctgcgc catctctata agggatagcc tcctgagact ccgttgtccc
    19621 tggaaaagct ggcgatggta tagcaccagc cggaatcggc tgtcaaccaa tccaggcgag
    19681 gtttgcctga aatccccgta gcctggagac gggtctatgt tacaatcccg ccccatgcac
    19741 cgcgtgagct atcagaggat ctcacgagct tgtgagccga tgcgcctgag aaaagcctgg
    19801 ctgctgggtc tcacctgcct cctgctggtc gcctgcagcg ccacgtcccc gctgattccc
    19861 gtcggggaag ggccattagg aacggtctcg cttgaacgcc tggccagccg gggcaccacg
    19921 gccagatacg gcagtcccca gagcgccttc caggcctctc atccggcctc ggtatcggca
    19981 tcggtaatct cacgcctgct ttccggcctc tccgtttccg gcctcgagcg gccgggtgcg
    20041 gccctgagac aagagcggta tccgctgttt acccaggaag aaatggagtt tttctcgccg
    20101 ctcatcgtca aggctctgtc ccaggccgga cctgaccaac gggtgcggtt cacccttcag
    20161 gacgacgggc tgatcaccca gggaacgctc tatctgcaca agaccaccct tcgggtcagt
    20221 ctttctcact atcgcgcgtc gcaggggcaa agcgacacca ggccgacagc gctcaccttg
    20281 tcgtttacgc catcagaagc actcgtgcga gacgatgtgc cgcaatcctg gatgattatc
    20341 gaaccggaac aaccccgtgt cgcggtgtcg ctcgaggttc tgaatgagct ccgcgccgcc
    20401 gtttccgctc cgaccgcgaa caaaccagtc gctgtaatac cagttgctcc ggccccggct
    20461 gcatcggaac agaccaacct gcaacaggag ctgcaagcca cgaaggatgt ggtcgtcaag
    20521 caggcgcagg aacttcagca gctcaagaca gaactggaat cgttgcgacg cgaactcgcc
    20581 gagagggact ccaccacgcc gaaagcgaaa ccgaaagcag ccccccgcaa aacggtcccg
    20641 acgccctgag ctaggtgctg acgattgtta caagtccgtg ggctcttctc ctgtcaataa
    20701 tgtcgaagcg caggccttgc cgggcgaggc ggtcccgggt gaacgccatc gcgcccttgg
    20761 ccagcatcaa tgccatagta cgagcaaaac ggaagccgcg ggcatgggat ggtgacttca
    20821 gaaatcaatc gagaacgtca cccgagcaac cgagagcgtc acagagtgca gcgcccgctt
    20881 cgctgaggga gcggtagaca cacgttcgac aatacatctc tccctattga gccagcggac
    20941 cagccaagac ctccttcgca tcatagacat catctgcgcc cgagggcttc ttacccttgt
    21001 acatcgtcac tgtcatagac gttcgccgcg aaagcgccaa cacgtctgcc ctctgcgcga
    21061 gatggaagaa gttgtatccc tcgcgcaacg tcagttctgc gcagcgccgc cgcaaatacg
    21121 aatcgagctg ctcctgggag gtaaaggaac tgacgcgata ttctacgcgg tagacattct
    21181 ccctgagctg ctcgacctca tctttgtcgg ctccgttgag cggctgatac tgtccggtgg
    21241 tgcaagcggc tacagtgaca agacatgcta tgcaccacgc cttacgcatt gccttcacgg
    21301 ttatcctcct caggcgggca aagaatgaat cacccgacaa cataaaacat gctggaccgc
    21361 cggcttccga agccaactgt actgagaaac cgcacgatcc ttattacgcc gggatcggca
    21421 ctccgcactg acatctccgc tccaccctct gctgatgaag aagagctcca ggctcgtgtt
    21481 cgactgtgac aagctggcaa atgccgcact gtgaacgccc catcgacacc ggttgctcac
    21541 gcgccgactc ctgaggtcgc actcgcctcc atacccgcca ccactgcacg acgatctgca
    21601 acacatcaca aaaggccaga tgaaacagaa ggaacgagag gtagaggagc tgcgaaagga
    21661 actggaagac atcaaacgtc agctgggcaa cacctcgacc caaggcggcc agccaccctc
    21721 aagtaggcca taacatccga agatagtaga actgtccggt catccccggt ttttatcggc
    21781 ccggtcttcg atctcgcccg ccaacaaaat cgcctcggca atctcgcgca tggacttgcg
    21841 aagatccatg ctctgtcgct gaatcaactt gaacgcatct tcttccgaga gccgtttcga
    21901 ccgcatgaga tagcccttgg cccgatccag taatttgcga acctgcaagg cttcctgcat
    21961 ctcgaaggac ttttccagga gcgtcgtgtg ctcgatggat atggctgcct gattggcgat
    22021 ggcctgcatg agtttcacat cctcggacgt gaaggtatgg ggcttggagg tatacgtatt
    22081 aatgacaccg atggccttct cacgcaccag catgggcacg caaaggagcg aacacagccc
    22141 ttctttcgcg gccatgtccg ggtacatgta ggagccttct ttggtgacat ccgggacaat
    22201 gatcggtcgg cgatcctgaa ccgcccgccc gctgatactt tggccgatct tcacattggg
    22261 cttgcgccgg tactgctcgc tcaaactttg cgtggcagca atgcgcagtt caccggtcgg
    22321 ttgatccagg aggataatgg aacagatttt cgagcccatc atttgcgccg tcatcgtcac
    22381 aatcagctgc aacacgtctt ccattaaacg attcgacgag accgtttcgg acacttggga
    22441 caaggtatca agttgcagcg ctttgcgggt catctgatcg tagagccggg cattctcgat
    22501 ggcaccgccg acttgcgtgg cgatggtcga caacagcgcc aactcatcag ggcgataccg
    22561 ccgcgaccgt ttgtgctgga cgttgatgac gcccacgacc tcctgcttgg tcgtaatggg
    22621 tacggagaca aacgcctgat aacgatcttc cggcaggtta tgaaaaaact tgaaccgaga
    22681 atcatcgctg gcgctattgg gaatcaccac gcgagttctt tcccgcgcga cccagccggt
    22741 gatcccctcc cccaatccga tcgtgatacg gccaatgagc ttcggatgag gatttttgga
    22801 ggctcgaagg atcagctcgt cccggttatc ggacaacagg tacagcagac aggcatcggc
    22861 tttggtcgct tcaaccacca catcgacaat atgccgaaga acggactcca gatcgagcgt
    22921 gctactgatc gattcgctga tgcggtggag gacatcgact tcgcgtgttt tttcacgcaa
    22981 cgcctgctcc aattgaggga ctgttcgctt cgatgccatt gcgctcctac cgccgccgct
    23041 tgttgttcgg aatgaattgc cgggaacggg tgttgtcact gctgaccgtg tcataccagg
    23101 cgcgagtctg ttccttcgct aacggaacga tgcggaccga tccgatccgt tccgggacca
    23161 cacagtagac ggttccggca gacacctttt tgtcctgttg catcgcatcc cataacgcgc
    23221 ggaacggcac cttcggaacg cgcaccggta gaccggccgc tgcaaccagg gcacgcaggc
    23281 gggtcacgac atcctcatga caatagccca ggtgccttgc cagatccgct tcatacacca
    23341 ttccgacggc gaccgcttcg ccatgaatca ggccacggta tcctccaaga gactccaatg
    23401 catggccaat ggtatgcccg aaattcaaga tacgacgccg gtcggcttcg cgctcatcct
    23461 ccgacaccac ctgagcctta atttcgcagg aacgcttcac gatgtgtgcg acgggtgcat
    23521 cactcagcat caaaatcggc gcgatatgct cttcgagata tttgaaaaac gactcatcct
    23581 caatgacccc gtatttgatc acctctgcca acccggcgat ccattcccgc tcaggcaacg
    23641 tacgtagtgt ggcgggatca atcaggacgg ctcgcggttg attaaatgcg ccgatcagat
    23701 tttttccttt gggatgatcc acccccgttt ttccgccgac gctcgaatca acctgcgcga
    23761 ccaggctagt cgggacctgc acaaagggaa tcccgcgctg atagatcgct gcagcgaatc
    23821 ccgtgatatc gccgatgacc ccacccccca aggccagcag cgttgaactg cgttcgaagc
    23881 gggcgttcac cagagcatcc atcaccgttc cgatggaccg cagggtttta gtgcgttcac
    23941 ccggcggcaa cacgatggga actactgtat atccggcagc cttcagcacg cgtatcacgg
    24001 gcttcaaata atgcccggcc agattccgat cggtgacgac gcccaccttc ccggcgcagc
    24061 ggagtcgcgt catttccacg ccgatctcct tcagcaggcc acgcccgatc tggatgtcat
    24121 aactccgcgc acccaattca agatggatcg tgtctcgagg catatcagga gtggatgttg
    24181 ccatgaactg ttatcccgtg acttggagag agcctcgtcg tcggcctcgc ctagcgcgag
    24241 cgcctcacca cgggcactaa ggtccttgaa ttacccggtc ggcaatggtg tggctcagaa
    24301 ccggatggtg aactgtacga ttcagaggga gaagaagccc ggaagaagcg ccatcgagaa
    24361 cggggaggag acacgttcca tgggattcgc ccccattgag acagcgtacc atcatcaatg
    24421 ggggcgatta ttagcgtgtc gagcggggac ctgtcaagtt gcggagcgca cgatcgtcgg
    24481 agtcaggaag atcaacagtt cctgcttcga gacgttctcg gtcttgttct tgaacagcca
    24541 gcccaggacc gggacgcggg acaggtacgg gatacccgcg acgttgttcg attgcgtatc
    24601 gacaaacacg ccgccgatga ccatggtttc cccgtcgcgc accagcacct gagtggtggc
    24661 ttcgcgacga tcgatgctcg gaccagcggg gttgcttcgc gcgccgacgg cattcctcgt
    24721 ggcccgcact ttcatcagaa tttgcttgcc gatttctttc ggatcacggg acgtaatttg
    24781 cggcgtcacg ttcagttcca ggttggcgtc cacaaatgtg gtctgagttc cttgcaagga
    24841 ggtcgtctgg aacgggatcg attcaccttg cgcaatcttc gcatcccgct tatccaaggt
    24901 cgtaatcttg ggcgcagcga tgaccttcgt caacccgagc aactcaccgg ccgacaaccg
    24961 cacatccaac atcgcaccat ccgttttgcc gatcgagaat ccggcgccag gcacagacgg
    25021 caatcccggg ttagccggca agttgatcag gaagtccgac acctgcgcac cgaaggctcc
    25081 tgtggtgccg gtcttgaaag cggaggttcc gctcgaggcg cccaactggt tgacgttctg
    25141 aataccccac tgcacaccca acgaccgggt atacgtcgtg tcggcttgaa cgatgcgcgc
    25201 ctcgatctgc acctgcggca cctcgagatc cacgccgtcc agcaactgcc ggaggacttc
    25261 cagcttggac tccgtgtcgc tgatgatcaa ggcattgctc gcagcgctga cggtcatggt
    25321 gccgcgtggg ctcaggttct gccgcaagga ggtttgcact tccgtcgcgt tgagattgcg
    25381 gatatagaac actctcgtga cgatgggctc agccttcgcc ttggaatcct tggcgcgcgc
    25441 ctcttcgtcc tgctgcttgg caatattctg cagagtatcc acccacacga tgttgccttg
    25501 gcggatcatg cccagagcgt tcatcttcag cagcatgtcg agggcctgat cccagggtac
    25561 attggccaac ttcatagtga ctttgttctt cacaccctcg ccgaccacga tattgaagcc
    25621 gctgacttca gcgatcaaac gcagcacatt gctgatgtcc gcctgctgga aatcgagaga
    25681 aatccgccgc ccgacaaagc gggacgaacc gtttaccact tcgttcgtct ccggcttccg
    25741 gtcttccttt tgaaccgact ccgccgtcaa ttggatgggc tgcacacgga acaacgcatg
    25801 gggcgtctta tgggagacta ccatcgagcg cgctgcagtc tgttcggccg acggcgaggc
    25861 ctcgctctcg gccagcttca catcattggt gggcacagac ttgggttcca gcatgagaac
    25921 caaccggccg tcgactgagg tgacgctgtg ctccgccggc tgggccaggt caaggaccaa
    25981 tcgcaccttg tcagcgtggt agccaaatcg cactttcgac aacaacgcat gaccgacggc
    26041 caactgcgtc tggcgtccct gcgattgaat gtgcggcata tcgagcacca tgcggcgatc
    26101 accgaccatt ttgaccgtgt ggtcgaacac cccgttaccg gtcaggatca gcgccactcg
    26161 tccgccttca ggcttggctt caacgctcgt cagcaatgtt gcggtcggag tggccccggt
    26221 ggagtgctcc tccttcgctg ccgtgaactc agcagaagtc gcctgatcag tggcctgcgt
    26281 gaatccggcc agtccggtcg tttcccccgg tgcggcggct tgcacgtagc ccgcaaggcc
    26341 taacagtccg gccatcacag tgacactcag cagcggatgt acaccaacct gtgtttgttt
    26401 cattctgtgc cctctttcgg gtgcaggagc ttgacgtatt cgcgctcctg tttattcccg
    26461 tacacgtccg tgaaccgctc ttgaacgatg atgcctcgtt cggtgattga actgaccaca
    26521 ccattgttag gcccgattcg tgttccccga cggatgctat accccttgcc atccggcgtt
    26581 tgaaccatgg ccgtgtaccc gtagttgccc cacagcacac cgatcagact cagttccgtg
    26641 aggccgacac gctgcagcgg gggaagactc gcatcttgct ccacctgctg gcccagctgg
    26701 accatcggca ggaaggggtc tcgacgtccc gaaggatcgt aggaggtccc ggaaaactcc
    26761 atcgacagcg aatcacccgg ctgcgagacc gacgcctcga cacgaggagc cggcgattcc
    26821 attgcaggag ccggcttctg cccctccggc agcggcatga ccttcaggga gtcggcgggc
    26881 cgcatggagg cgatctgccg caagtgcggc agggatttcg actccaccgg catggcagta
    26941 ccgccaataa ccagcactgc ggctccgatg ctcattttcc agcaagttcg tgtgttcacg
    27001 gtacgtcctc ttacattgga catgcgctgg gctccattcc tcacttcggc ttcgccgcag
    27061 gcaccggcgc agccggcttc ttctcctgcg gagcggcata ggcgaccaga tcaaagactg
    27121 tttgcgtcac cacacggcct tggtcgaccc gggcagcgcc gatccggaca tcctgaacgg
    27181 tcacgatacg ggacaacttg ctgattcgat cgaagaagat ggcggctgta tggtacccgc
    27241 cggccacttc tacgttgatc ggcattcgaa tgaacaactt cgaaggatct tcggcttgcg
    27301 tgcccggctt ccacaacttc acatccaggc ccaaccgcag tcccagatcg gacacctgct
    27361 tcagcaacat gacggcttct tcttccggcg ggaggcgttc cttcttcgcc gccaattcga
    27421 tctccaactg cttattggcc gcgaccagct catccaggtg tttgaccttg agcgtcaggt
    27481 tctggatctc ggtatccaac tgagccacct gcccttgcac ggcttccagt tcgacggtct
    27541 tgggatcgac gacgtagaaa taaaacccga ctaaaatgcc tgccaccagt aagccaagca
    27601 gcgcaacctt ttggcctgtc ggaatgctgc ggaggctgtc taagctgatc gcattcagac
    27661 tcatgttagc ctttcatcga caggtcaagt cggaattgat aggtgttcat cttattgtcc
    27721 agcccagccc ggctctccat caatttgatg gcgccgaatt gatcggtccg ccggaggtta
    27781 ttgacgaatt caacaacgtc atcgttcgtc aaagcccggc cctcaagctc aacgctgttc
    27841 cctttgagat tcagccgcac cagccagact ttcaggggat tcaggctctg gctcacatga
    27901 tccagcactt tgaccgggcc cgtccggctc ttctccagct gatcgataat gcggttcttc
    27961 gcttcgagtt gctttttctt atcttcaaag tcctggactg ccttcacctg ctctttcaac
    28021 tgcgccactt gcttgtcctt gagctgcttt tcgctttgct tggcctgaat gtcttcgtcg
    28081 agcgatgagg cgtagaacca acacccggtc aacgtgatca gcagtactcc gacgccgatg
    28141 agtgcctcgg cgcgaacatc ccactgcggc ttggctttcc tggaccgagg tccagttgcg
    28201 agcaagttga ttctaatcat cggtccccca ccgtccgcag tgcgaggcca acccccactg
    28261 ccgcaagcga ccccatctcg gccaactgtt caggatcgac attgcactgt gaggtgtcga
    28321 tctcattaaa gggattcgca atttccacat cgacatgcat ccgatcgcga agctgctgag
    28381 ccaacccctt caccttcgct cccccgccga acaacaagac ccgcgcaatt tcgctctctg
    28441 aggacgtggt cttgaaataa tcgatggtgc gtgcaatctc cgatgccact tcggcattga
    28501 cgctctccat caccgccgca agtgtatgat cgtcatctcc gtcgtttttc ttgagctgct
    28561 ccgcttcctc gtacgacacg cccatttctc tctgtacggc ttccgagtag cgatttccgc
    28621 ccagagggat atcacgcgtg aagagcgaca ctccgccgcc gacaatgttc acattcatga
    28681 cgctggcgcc gatgttgacc agaatggtgg tgtcctcttg agtagagggc gaggtcacgc
    28741 catacatgtt ttctacggca aatgcgtcca catccatcac gatcggcacc aagccggccg
    28801 ccttgaccaa ttccgtgagt tcgttgatct tgtctttctt cgcagccacc agcacgatct
    28861 gcatttcggt ctgctcgtcc gggttttccg acggcggcaa gacgtagaaa tcgagattga
    28921 cctcattaat atcgaacgga atgtactgtt cagccgccag cttcacttgg gcgtcgagtt
    28981 cctcgtctgg catgggcggg agagtaatct tcttgacgat gaccgcatga ccggagatcg
    29041 agacggctac ctgcttcaat ttcacgttgt gctccgccag caattccttg atggccgaga
    29101 cgacccgccc ttcgtccatc accgtgccgt ccacaatcac ctcgggctcc agttccttca
    29161 cgccgaactt ctgcaaggtg tagcggcccc ggtgctcctt gatctgcacc agcttgatgc
    29221 tgctcgatcc gatgtccagc cccaaaagct gtttctgggg ggaaaacatc gacagaaaat
    29281 ccatgtccgt gaggtgtttc agtgaagcca gcatgccttt gccctctcta cgcaacgtct
    29341 ttgcgcccgc cacccatcac atgcttgatc tgttcgacat tttcggccgt atagagacgc
    29401 caatttcgcc aatctcgcgg cggcccggta atcaaccctt cgcgctccca acgaaacaac
    29461 gtcgcgcgcg agatgtcgaa catctcgcag acctcgttgg tcttgtagag tttctttttt
    29521 tgaccatcgt tcactttctt cacgcaccgc ctcaaacgcc actattcatc gcgtaattcg
    29581 agctcacgaa attaagtata gttgatatag tccacctgtc aagaaagtgg cctccgtgag
    29641 ggcagacagg agaaaaatgg ggaaaagacg ctctgggact aggatttctg caggtcgcgg
    29701 ttggtcacgg atacgtcgta accgagggcg gcaaattgtg cctggaggcg cagcgcgatg
    29761 gtcaccgacc gatggcgccc tccagtacac ccgatgccga tactgaggta gctccgttga
    29821 tcccgttcat acagtgggat cagaaaggca aagagttttt gcagatgttc gaggaactgg
    29881 ccggcggtct cgtctccgaa tacgtactgc tgcactcgga gatcttctcc cgtcagggct
    29941 ttcagctcgg ggacaaagaa aggattgcgg atgaagcgga cgtcaaacag caggtcgata
    30001 tcgtagggca caccgaattt gtagccgaat gtgaccaatg aaatggtcat gcgtcgggca
    30061 gtcgtttcct gacgaaactg cctgatcagc agatcgcgca gttcgtgcac cgtcagcgtg
    30121 gaggtgtcga tcactcgatc agcatgtttt cggagttcgc tgaggcgttc gcgttcgaac
    30181 cgcacccctt ccacaacagg catatgcggc aacagcggat gcgggcggcg actttctgag
    30241 aaccgccgga ccagcacttc ttcgcgggct tccagaaaca ctaactgcag ggcgtgtccc
    30301 tgcgacttca attcggcgag tacttttgcc aggtctccga aaaatacccg ttcgcgaata
    30361 tcgatcccca gggccacatt cttaatctcg ccgccctgct ggtggcacaa ttcgacgaat
    30421 gtcggaagga gggccggagg aagattgtcg acacaaaaat agcctgcatc ctcaaaggct
    30481 ttcagcgcgt gactctttcc ggacccggaa agaccgctga tgacaaccag attaagcgcg
    30541 gccatgtgcg acgcgagcac ggtcgctcgc aggatcaatc agggacagtc ttccgtttgg
    30601 cagccgttgg agcacttgaa tagacccgga aagcgcgttc acaaacatca aaagcggcag
    30661 cttctcccca ttgagcgtct cgaccgcttc ctcgatcgtc agggcttcga gcctgggacg
    30721 gaccacttcc atgccgtcat cttttgcgcg cggggtcttg cgagcgacgg cacgtacgcg
    30781 cggctgatcg ccgaatttgt gattgacctt gcgttccttc aacttgcgta gctgcgcgcc
    30841 gagtttatcc acgacttcat caatcgacgc gtacatttct tcggtcgtgg ttttggcttg
    30901 cagccggcgt ccctgcacga cgcccacgac ctccgccaca tgatgaaact tttccacact
    30961 caacaaaatt tggagcgtgc ccagcttcaa cccgtagcgg tcgagccgct ccatgcgtaa
    31021 ctcaatatac tctcgcaacg ccggggtcac caccacatgc cgtcccgtga tcatcaaacg
    31081 catgccatcc tcgtttctcg ggccttgcac gcaaggctgc gttcaagccc gttgcgacga
    31141 ttgttgtacc gccatagagc catctccatg gtgccagact tcgcgcacct caaaaaaact
    31201 tccgccgttg cgtggcagag ggaatgttat cctctgcgcg atatttggct acggtccggc
    31261 gggcgatcaa tacttgttgg gtccgtaatc gggcggcaat ttcttcatct ttgagcggct
    31321 ttctcgcatc ttcagccgca accatttgcc ggatcatttc ccgcacggtg agtgaggaca
    31381 gcatatccgt cggttgatcg gcgcgttgca atccagcatt gaagaaaaat ttcagctcca
    31441 gcatcccctg cggacaatac atatacttgt tggccgtcac ccgactgatg gtggactcat
    31501 gcatgccgat atcttcagcg acctgtttga gaaccaatgg cttcaggtgc tgaatcccat
    31561 gttcgaaaaa cccttcctga aacttcacga tgctggagac aactttcacg atcgtcttat
    31621 tgcgctgctc gatgctccgg atcacccact gcgcggcgcg gagcttgtcg tccagatacg
    31681 ctttggtttc cgccgagccg gactggcctg aggacatcaa ttgcttatag tacgggctga
    31741 tccgcatgcg cggcaggcca tcatcattca gcaagacctc ccactcccct tcgttcttga
    31801 cgacgaagac gtcgggcaca attgcgtaat tctgggtgtt ggagaaggga cgccctggct
    31861 tcggctccaa gccttcgatg atgcgagtcg cctcgaatac atcgtccatg gaaatgttca
    31921 aggccttggc aatacggtta tattgcttct tttcgagatc cttcagatga tgcaggacga
    31981 tggcttcaag caccgagccc ttcagcaccc caggccgaga ccccagagag cccagttggc
    32041 tccgccctaa aaacttgagt tgaagcagca ggcactcgga caagtctctt gccgccacgc
    32101 cgttgggatc gaacccttgc acatctttca gcacggattc ggcttccgct acggaatatt
    32161 cggatcctgc caccagttcg tccagcgtca tccggagata gccgtcatcg tccaaattgc
    32221 cgatgatgag gcggccgatg gccttttcac gatcggataa gccggacaac gacagctgcc
    32281 agaccaggtg gtcttcgagc gacgtcgact tggccaccgt ctgctcatag gagggaaact
    32341 cttccttgga tgaggagctg tattccgttt cgccacggcg catgtcggta tcgaaatagt
    32401 cttcccaggt agaggcggaa aactcttcgg cattttctcc gctctctgcc ggcttgtcat
    32461 cgccttcccc gcgcgtctca gcggccacag tcgtcgccgt atccggttgt tcgcgctcag
    32521 tggctgcagt ttcctcgctc tcctccgtct ccgccaccag ttcgtccagc aaggggtttt
    32581 ccatgaggtg ctgctgcaga ctttgttgga gctccaagcg ggacaattgc agcagcttga
    32641 tcgcctgctg caattgcggc gtcataatca gtttctgact aagccgaaga tccagtcgca
    32701 acttcatcgc cccactccct gcctgctaca ggcgaaatcc ttcgcccaaa tacaccgccc
    32761 gagccatttc acttttctcg atcacgccag gaggaccggc ttcgagaatc gtgccttcat
    32821 tgatgatata ggctcggtcc gtaatcgaga gggtttccct cacattgtga tcagtgatca
    32881 ataccccgat atgccgttgc ttcaatcgaa tgatgatttg ctgaatatcc gcgaccgcaa
    32941 tcggatcgat tccggcaaag ggttcgtcca acagcatgaa gagcggactc gttgccagcg
    33001 cgcgcgtaat ctccaatcgc cggcgttccc ctccagataa ggcgtaggcc ttactttttc
    33061 gaatatgcac cagatcgagc tctttcagca acgtttccac ccgctccatc cgttcacttc
    33121 tcgaatagcc tagcatctcg agaatcgcaa ggatattgtg ttccaccgac agccgccgaa
    33181 agacggacga ttcttgaggc aagtaaccaa ttcctcgccg ggcccgctta tacataggca
    33241 aatcggtaat gtcgttgcca ctcaacgcaa tctgtccctc atccggctga cacagtccca
    33301 ccatcatatc gaagatggtg gtcttcccgg ctccgttcgg ccccaacagt ccgaccactt
    33361 caccggcctg cacgtcgagg gagacgccct tgaccacctt gcgcccgcgg aagctcttca
    33421 ccaacccgct ggctgccaag cgatccgctt tcgccgataa cgctgccacg ccttcttgcg
    33481 gcgtctggct ggtgattggc ttggtgtcaa tcaccgcttg ccaccgcctt caccttcgat
    33541 aacgacctgg gagtcgccct ccaccacact tcgatcttcg tccagaaaca taatgatgcg
    33601 ctttccggac acacgcgttc ccttctgcca ggccacaggt tcgcccgtga gcacgatttt
    33661 tttctgctcc tcataataca cggccttctg gcacgtggct tggccgtctt ctttcttaat
    33721 gttcacacga ccggtcgcct cgaccatgcg aatactgcgt tccgatacgg caggcgctgc
    33781 atctcgctcc ttcgattcgg ccgtcgtcgg gttcgtgaga ttcttggatt ttgtcgggcc
    33841 tccggattct ttaccatcgg cgccattgcg gctcgggtgg aaagagacca ccatgtgatc
    33901 tgaatgcaca accaacggac cacgcgtcaa gacaacggct ccatcaaaaa tcgccgtgtt
    33961 ttcttgatta ttgacggtca tcgtccggga cgtaatcgta gtaggggcag aactggctgg
    34021 aggctctttg gtcccgcgcg tttctatcgc gtacgcgttg tgcgtgctta gcaccgcctc
    34081 gccgctaaga agcaggcacg acatccacat gcacgtcgtc aaggatcttg aattcctccg
    34141 tatccatctt gcccaacaac cctcttcccg tgacttgcaa tccatgcccc agaatcgtga
    34201 catggtctgg agtttgaatt tcatgccgtg cgtcagtcca tacaaggtga tttgtctgaa
    34261 tggtatagcc actttgtgtc tcaactacaa tcggcgtgga tctgttcgac aactgaaagt
    34321 tcttcgtttg tgtatccagc gtcccctcgt ccccggatag ggagagttcc tttccctgcg
    34381 caccgtacat ggtaatctga acattgctga ggatagcgcg gctctctttt tcgtagaggc
    34441 gcgcttgctc ggcttgcact ttccactgaa cgagatcgcc tttggtctgg gtaaaggtga
    34501 agtcttgaat atgcgcatcg gcagattcgg cctcgaccgg cgcgaccgat cgcgacgaag
    34561 agctgggatt ggagcgtgta atgagcaaat acccaaggaa tgccgcaaga actacgctga
    34621 gagctaagag acctcgccga acccaacgtt cccacatgga aaaaagcccc tattttaaag
    34681 agcttcccag atcagagtct ggtggcatga tagtagcatt ccccagaagc caagtaaaca
    34741 aaagggacgg cggatggcct gagaaaatgg tgtgagttgc aagagaatcg aggcagggag
    34801 gaaccgaagg ccacgacgac agtgacgtca tcgtggcctt cttctcgtta gctttctgca
    34861 ggcgctgaac ttttctcagt cgactctttc ttctccgcca ctgacgtgtc agcggcaggc
    34921 gctgccttag gcttttccgg tcgtgtaact aactctattg cgaccatctc agccgcatct
    34981 ccgatccgac ggcgagtgcg gatgatacgc gtatagcccc caggacggtt ttggaaccgt
    35041 gacgcaacgt cgctgaataa cttcgacact acggccttgc tccgcaagaa gctcaatgcg
    35101 cgacgacggg caggcagggt gccttctttg ccaagggaga tcattcgatc agtgaaacca
    35161 cgaatttcct tcgccttcgc ttccgtcgtt tcgatacgct cctgctccag caatgaagtc
    35221 acgagacttc tgaacagagc ccatcggtgt ttggtttgac gtccgagctg tcgacccttc
    35281 tttttgtggc gcacggtagt cctgttcctt tcttactctg aacgcacgcc cgcgtcgcct
    35341 gacggcaagg cgtcgacttt cattccaagg ctcagtccca tttcggtcag gatttccttg
    35401 atttcgttga gcgacttctt cccgaaattc ttcgtcttca acatttctgc ttcggtcttt
    35461 tggaccagat cggcaatggt cttgatattg gcgttcttga ggcaatttgc cgcgcgaacg
    35521 gacaattcca attcattgac gcttcggtac agattcttat tcacttcatc ggacaaatct
    35581 tcgctaccag cggatggctt ggcgtctgaa cgttcttcag gattgatgaa gatatcgaga
    35641 tgttcccgaa gaatcccagc cgcgttggac aatgcatccc gcggagaaat cgtgccgtcc
    35701 gtccaaattt caagcgttaa tttgtcatag tccgtcattc gaccgacgcg agcattttcg
    35761 acgtggaaat ttacccgttt gatcggagag aaaatggagt cgatggcaat cacaccgatc
    35821 ggcaggcctt cttccttatt gcgctcggct ggaacaaacc cgcgaccatg cttgatggtc
    35881 atttccatat caagcgcagc atccttgtcc agcgtcgcga tgtgcaggtc tggcgtgaga
    35941 atcgtcacgt caccatcatg cgtaatatcg gaacctttcg cttctcccgg tcccttcttc
    36001 ttgagacgga tggtcttcgg cttgtcgccc tgaagcgcca agcggagact ctttacattc
    36061 aaaataatcg aggtgacgtc ttccgtcaca ccaggaatcg tggaaaactc atgtaacaca
    36121 ccttcgatct tgacggtggt caccgcagcc cctgtcaagg aggacagcaa gacacggcgc
    36181 agggagtttc ctaccgtggt gccgaatcct cgctcaaatg cctcggtcgt aaatcttccg
    36241 aatgtcgggg aaagagtgtc cttgtcgact tccacccgca tggggatctg aaagtctttc
    36301 atcgctttaa tcatgactcc cccttcatta actcgtgtgt gcccatatat cgttcaacca
    36361 tgatgaggtc caatcaggga atccccaacc atgtcggttt agcgcgaata cagttcgacg
    36421 accatctgct catttacagg caacacaata tgttccttgg tcggcaagga tcgaatcgtc
    36481 cccttgtagg cggccctatc caactcgagc cattcaggaa tgccacggcc gtccaccgcc
    36541 gcaagtgcac cctgaatcgg aatcaattcg cgactctttt gccggacctc gatggaatca
    36601 ccagctttga caagagcacc gggcgccttc acttttcggc cattgatcat caagtgacca
    36661 tgattgacca actgcctggc ctcttttcgt gaggcgccga agccgagccg atacaccaca
    36721 ttgtctaagc gacattccag gaggcgcagc aaggtatcgc ccgttacgcc ggcttgacgt
    36781 tccgctcgct cgaaaatgcc gcggaattga cattcctgga gaccgtaaat cctcttcagt
    36841 ttctgctttt cacgcaactg taagctgtag tccgacgtcc gttgacgggc ctgaccgtgc
    36901 tgtccaggag gataactccg gcgctcgatc gcgcacttct cggtcatgca acgagacccc
    36961 ttcaaaaaga gcttctcgcc ctctctccgg cacaagcggc agacaggtcc actatatttt
    37021 gccacgctac tacccctcct ctacctcaac aacttaagta caaccgactt agatatttcg
    37081 ttactgcgaa gatgcccgga tctctcctac acccgccgac gtttcggcgg ccggcatccg
    37141 ttgtgaggaa tcggcgtcac gtcgcggatc aaattgatac gcaatccggc actttgcaat
    37201 gagcgaatgg cggactcgcg tccagcaccc ggcccattga catagacatc tacctggcgc
    37261 atgccgtttt ccatggcttt tctggctgca gcttcacccg ctcgttgcgc ggcgaatgga
    37321 gtactcttgc gtgacccctt aaacccctga ttgcctgaac tggcccacac cacggtgttg
    37381 ccgctcatat ccgtaatggt cacgatcgta ttattaaacg aggcctgaac gtgagccact
    37441 ccgctctgaa caatcttccg ttccttcttc ttgcctttct tcacactcat agaatctccc
    37501 tcagcgcatg gcgcctgatc ggtctaatct cttatgctct ggaaccagcg ggcttctgtg
    37561 gtttgctgcc gacgcccgag cgtcttcctt tacgggtgcg cgcatttgtc ttggtccgct
    37621 gtccgcgaac aggcaaccct ttgcgatgcc gaagcccacg aaatgttccg gtatccacca
    37681 aacgcttgat attcatcgac acttcttttc gcaagtcacc ttccacgcgg taatcccgtt
    37741 cgatcacctc gcgaatcttg acgatcttgt cttcgctgac atctttcacg cggatggccc
    37801 catcaacccc agccttttta agaatcgcct gggccgcaac gcgaccgatt ccaaacacat
    37861 aggtcagacc aatttcgatt cgtttgtctt ttggcaaatc aacgccagca atacgtgcca
    37921 taattacccc ctagttcctt ttcttgctcg cctgatgagc tggctcagcc acgttcagag
    37981 attcgccctg ccgttgttgt gcagacgact ccttccagac tccgcgcatg ccgtgccaag
    38041 gcaacccacc gccgctcatc cctgccgttg tttatgacga ggattggcgc ataagacgcg
    38101 aacgacccca cgccggcgaa ccaccttaca ttttgcacaa atcggcttaa ctgacgactt
    38161 gaccttcata gcaattccat ttctccgcta cttgaaacga taagtaatcc gtcccctcgt
    38221 caaatcatag ggtgacaatt gaatggtcac cttgtcgcca ggaaggatcc gaatgaaatg
    38281 catccgcatt tttccggaaa tatgagccaa aatccggtgc ccgttttcca actctacgcg
    38341 aaacatcgca ttcgggagag tctcagccac cgtgccggtc acttctatga catcttcttt
    38401 tggcacgtat tggtacgcct tcccgtctta gtgtgacaat tgtgaaagaa tcttggccgg
    38461 tccacttggt tgaatcgcga tggtgtgctc aaaatgagca gaccagcggc catcttgagt
    38521 gaccgccgtc caacgatctt ctaaaatgcg aacggcgctg cctcccatat tgaccatggg
    38581 ttcgatggcc aataccatac cgacttggag ccggggacct tgcccaggtt tgccataatt
    38641 tggcacttgc ggctcctcgt gaagctgcct tcctatacca tgaccgacaa attccgtcac
    38701 aacggcaaaa ccggcctgct cgacatgggt ctgtatagca tgagacacat ctgaaaggcg
    38761 attcccgacg actgctttcg caattccctg gtacatcgct tcttcggtca cctgtatcag
    38821 acgtaacgcc tcctcacttg ccgatccaac cggcacggta accgcggagt ctccatagaa
    38881 gccggaaacg atggcgccca agtcaagccc aataatgtcg ccttccttga gtttgcgttt
    38941 cgatggaatc ccgtgaacga cctgttcgtt aatcgatgcg cagagcgttt tgggataatt
    39001 ccggtatcct ttgaaggctg ggatggcgcc acgaccacgg atggcctctt cagcgatccg
    39061 atccaagtcc tcggtcgtaa tgccaggttc gactttcccc ttcaacagcg cgaggacttc
    39121 tgccaccact cgggaagcct gcgccattaa tgtcacctca tccggcgttt tgaagacgat
    39181 cattgagcgc cgagcccgag cagcaacgcg gaaagcctcg tgtagacagc ggccatttcg
    39241 ccagagccat ccagatcgct caggaggttc tttgcttggt aatacgcaat cagcggggcg
    39301 gtttgctcgt catacacctt caatcgagcc tcaatcgttt caggcttatc atcacttcgc
    39361 tggatcaacg acgctccgca ccgatcgcac tggccctcga ttttcggtgg agcaaaggcg
    39421 atgtgaaaca cgctttgaca tttagggcag ctccgtcttc cactgagacg tgtgaccaca
    39481 tcatgccggg aaacccggaa gttgataacc cgatcgagct tcagattccg gccggtcaag
    39541 agagcctgta attcttcagc ctgcggaacc gttcgtggaa aaccgtccag cacgaagccc
    39601 ttctgacacg aaggcgcagc caacgtctct cgtaccatcc cgatgactac gctatccgga
    39661 actaactttc cggcatccat gctcttcttg gcttcaatac cgagtgccgt ttgatttcga
    39721 acggcttccc gcagcagatc accggtagag atcttcggcc acccaaattg agcggtaacc
    39781 cgatctgcct gagtgccttt gccgacgcca ggagcgccga gaaaaacgag tcgcatgagg
    39841 tggtcctagg acgttctgcc tcgaagtttt cctttttgaa ggaatccatc gtaattgcgg
    39901 tttaggagat gtgactcaat ctgctgcgca gtatctaagc caactccgat aacgatcaac
    39961 agcgacgtac cgccgaaata gaacggcata ttcagcttat agataagaaa ttccggaatc
    40021 acgcacacga tcgccagata gatggccccg gcaaacgtga tcttcgtgag aacggaataa
    40081 atatagtctg aggttcgctg accgggacga ataccgggaa caaatccgcc atatttcttg
    40141 aggttgtcgg ccatatccac cggattcatg accactgccg tatagaagaa acaaaagaat
    40201 acgatcaggc cgacgtacat gagggtgtaa aggagtgatc ccggcgcgag ttgtgacccg
    40261 atagccttaa tccacggggt ttcaaaaaat ccggcaatcg tcgcaggaaa ggcaatgatg
    40321 gacgaggcaa aaatcggggg aatgactccc gcagtgttga tcttcaacgg aatatgggtg
    40381 ctttggcccc cgtagaccct tcttccaatg acacgctttg catactgcac agggactttt
    40441 cgtctcccgc tctcaagaaa gacgattgct gcgaccacac caaacatgac gaccgccaag
    40501 acgatcagga gcgggaagct taattgaccg accttataga gatcaaatgt ttgagccacg
    40561 gctgcgggaa gtcgtgccac aatccctgcg aaaataatca aggaaatacc gttgccaatg
    40621 ccgcgttcgg tgatctgctc gccgagccac atgaggaagc cggtgcccgc acagagggta
    40681 atcaccgtca tgaagcggaa tccccatccg ggactcagga cgaacgcacc ctggttcatc
    40741 tgttcaagac caacggcaat cccgaaccct tggattaacg caataacaat ggttccgaac
    40801 cgggtatatt ggatgatctt ttttcgaccg cgttcccctt ccttcgccag cttcgaaaga
    40861 tgcgggataa cgacagtgag taactgaaga ataatcgacg cgctgatgta gggcatgatg
    40921 cccaacgcga aaatcgtcag gcgtgaaaga gatccgccgg agaaaatatc caagaatccc
    40981 agaagcgcac caccttgctt ctgcagaaat tcggataagg cttcaccgtt gatacctggc
    41041 gtggggatgt gggcaccgac gcgatagacg atcagcatgc ccagcgtgaa caatacgcgg
    41101 gtccgcagct caggtatctt gaagatgttt tggaaactgg tcagcagacg ctcaaacaca
    41161 gggaatgacc tcggccctcc ccccggctgc ctgaatcttt gcctccgctg acttgctgaa
    41221 cttgtgagcc tggaccacga gaggttttgt caactctccc tgggcgagga tcttaatcag
    41281 ctctcctttg cgtcgaacaa ggccttcatt catcaagagt tggggcgtaa tggcttctgt
    41341 cgtttccaga ccagccaggc tttttaaatt aatgatcgta tattcagttc gaaattgatt
    41401 cgtaaagccg tactttggaa cgcggcgaat caatggcatc tggccgccct caaatcctgg
    41461 gcgctttccg cctccggaac gagccttcag acccttatga cctttggtcg ccgtcttacc
    41521 atggcctgaa ccagggccac ggccgattcg ctttggtttg tgcttcgctc ctcgagaagg
    41581 agccagatca tgcaacttca ttggggactc acttccagta aatagcctac cttggcaatc
    41641 atgcccttca cttgatcggt ggcaggacgc agcacagtgg cattaagttt gcgcagtccc
    41701 aagcccctca gtaccaatcg atgcttatag ggcgttccaa tagggctacg cttcagggta
    41761 attcgcagac tagcctgcgc agtcgtagtt ttcttttcag tcgccatgat gtccttacgc
    41821 ttcactacca acagcccgcc gtagtcgcat gatttcctgc ggatcgcaga gctgttgcag
    41881 tccattaaga gtggccctga cggcattaaa cggattaccg cgaccgaggg ttttggcgat
    41941 aatgttgtgc gcaccggcca actcgacaac cgctcgaacg gctccgcctg caatgattcc
    42001 ggtaccgtcg gcggctggct ttagaagcac gtgctcggca ccaaacagtc cgtggacttc
    42061 gtgtggaatg gttccccctt tgatgggaac gcgaaccagg tgcttcttgg cctgctcaac
    42121 agccttggaa atagctaccg ggacttcggc agcttttcct ttgccgacac ccacataccc
    42181 ttgcccatca ccgacgacca ccaaagcgca aaagttgaat cgctttccgc ctttgaccac
    42241 tttggcaacg cggttgataa acactacctt gtcttttagg ctcaattctt cgggattgac
    42301 tcgcacaacg tcattctcct ttaggtcgtc cgatttttct atcgggaagc ggaggcgttc
    42361 cgcctaacgc acttctaaaa ctgtaatccg ccttcgcggg atgcatcggc caaggctttc
    42421 acgcgtccat gatactgccg tccaccacgg tcaaacacaa ccattgagac gttggcggcc
    42481 ttcgctcgtt cagcaatcag ttttccaacg gctttggcgc cttcaatgct gcctgtcgat
    42541 ttcacggcct tccgcaagga ctcatccaag gtagaagcag cggccaccgt atgcccccga
    42601 agatcatcga tgatctgggc ataaatgtga gagcggcttc ggaagacgtt taaccgcggg
    42661 cgatcactcg tcccgactac agattttctt acgcgctgtt tacgccgtgc taattttcga
    42721 tttttttctt gggtattcat taaatgcctc tacttgccgg ttttacccgc cttcttcctc
    42781 agcacttcgc cggcgtatcg aattcctttt tgcttataga catccggggg tttaatggaa
    42841 cgaatgttgg ctgctacttg accaaccaac cgcttgtcga tcccttttat cgaaatgagt
    42901 gtttgcttgt cgaccttcac atcaatgccc tgaggcaccg tgtaggtaac cggattgatg
    42961 tagccgacat tgaaactcat ttcacggccc tgaagttgcg ctttgtagcc cacgcccgtt
    43021 atctctaacg acttctcata gcctttggtg acgccttgga taatattgct caactcggcg
    43081 cgaaccaagc cgtgcatggc acgaatctct cgcgcatcac cagaccgatt gacaacaagc
    43141 tgcccatcag caaccgcgac gctcaatcca tcggtcaact tccaatcaag cttgcccatt
    43201 ggccccttga ctgataccaa acgaccagcg accttcacat cgacgccgct tggaactgca
    43261 attggtttcc gcccaatcct cgacatacga accccgttac gttacagcga tctcggttat
    43321 actgcgtcct agacgactcc tcattaccag acagcgcaaa gcacttcacc gcctaatccc
    43381 gcgcgcctgg agtcactgtc ggtcataaga cctttcgagg tggaaatgac agccacgccg
    43441 atgccgtttc tcacctttgg cacatcatct ctcccgacat acacacgacg cccgggcttg
    43501 ctgattcgac gcatccccgt gatcattggg cgcgcttcct cgacatagcg gagctggaca
    43561 gagaagatgg gatgcccatc ttcctgcgtc tccccgtaac cgaggataaa tccctctttg
    43621 ccaaggatgt tcagtatctg tcgttttact ttcgacacgg gaactttcac gacatcctgg
    43681 cgacggcgag ccgcgttccc cagtcgaacc aacaagtcag caatcggatc agtaatcatg
    43741 cattcctctt ctatgcctgc aactgcaagc tttcacctgg gcgactacca gcttgactta
    43801 cgcactccag gaatctctcc cttgaggctc aaagatctaa aacagatacg gcacatacga
    43861 aatcggcgca aaaatcctcg cacgcgaccg cataagccgc agcggtgata gtcacggcac
    43921 gagaacttgg atttcactgc cgccttattc ttgagcgcta atcttgacac gagaatcccc
    43981 ctttactccg cgtggcccac aaggccgagc caacctctct gatagctgtt tttaggcccg
    44041 aaatggcatg ccgagatgtt tgagcagggc cttgccttca tcattcgtac gcgcagtggt
    44101 cacaatggta atatccatgc catgaatcga tgcgacacta tcgtattcaa tctctggaaa
    44161 aatcaattgt tcctttaggc ccagcgtata attgcctcga ccatcaaacg ccttggggga
    44221 gataccacga aaatctctga tccgagggag cgctaaggaa atcaagcgat ccaaaaactc
    44281 ccacatccta cgactacgca aggtcacctt ggcgccaatc ggcatcccct gtcttaattt
    44341 aaaaccggcg atagcctttt tggctctggt cgtgactggc ttctgcccgg taataatgcc
    44401 caattcgcta gccgcgctct ccagcaactt cacattttga attgcctcgc ccatgccgac
    44461 attcaggaca attctctcaa gacgtggcac ttgcatcaaa ttgccgtagc cgaattcttt
    44521 cattaacgcg gggatgacct gatcacgata ggtttcccgc agtctcggtg caaattgatg
    44581 ttcactacca gcctcctgtg aaacctcagg cgctgccgtt tccttcttcg tggaggctcg
    44641 aggggttgcc cccttaccgc ccttaccctt ctctactttt gccatgaacg tctttcctgt
    44701 tccgactatt caacggtttc ttttgatttt ttactcagtc tggttcgtcg cccatcttcc
    44761 tggcgcttga tccctaaccg agtaggcttc ttcgtgactg ggcacaagaa catgacattg
    44821 gaaatggcaa gaggagcctc acgttcaaga atgccaccct gccggagttt ttgattcggc
    44881 ttggtatggc gcttgatcat attgagtttc tctaccaaaa ccttccccgt tacggtatcg
    44941 acagacaata ccttgcctga cttaccacgc tcacgcccag tcatcaccac aacggtgtcg
    45001 cccttgcgaa ttttcgtttt gattcgtgcc atccctacca gaacctttct tccttcgact
    45061 acaagacttc cggcgcaagc gaaataatct tcatgaactt tttccagcga agttcacgag
    45121 cgactgggcc gaaaattcga gtcccaacgg gctccccttg agcgttgatc aggacgcagg
    45181 cattacgatc aaatttgata taggaaccat cgtcacgccg aacttccttg gtcgtacgca
    45241 cgatgactgc tcgactcaca tcaccctttt tgacgccggc ctgaggaatt gcctccttga
    45301 cggcgaccac cacgatgtcc ccaagcgatc cgtaccgacg ccgagtgcca ccgagcacat
    45361 gaaagcacat gacctgcttc gcgccggaat tatcggccac atccatgtat gtataattct
    45421 gaatcatacc ccgccctact caccgccaaa aggatcaggc ccttcagctc ttcaggtgtg
    45481 ttccgtagtt acttttcagg ttgtcccttg accatgacgc gaacgacgcg ccaatgcttg
    45541 tccttactga tgggacgtgt ctcactcagc tgaacgcgat cgccaacctt acacacattt
    45601 ccttcgtcgt gggccttgag cttggtgacc cggcgaagaa ccttcttgta aattgggtgg
    45661 atgaccgatc gttcaaccgc cacgacaacc gttttattca tcttgttgct gacgacgttg
    45721 ccgtaccact cacgtcgatg ttgttggcct tccttcatac gccccttctc tcttactttc
    45781 cgaccgactg gccggcctga gtctgcagca attccaattg acgcctgacc gttttcaggc
    45841 gggcaatgtc gcgcttcgtg cttctaacct gcattgggtt ttcgaggcga ccaatgccga
    45901 gttggaatcg gaaattaaag agttcctggc gcaattgttt ttcctttgcc gccaactctt
    45961 ctagcgagag cccgctgaga tccttcacat ccattacatc accactcaac actcgctaca
    46021 ggttactgaa actcgccgcg caccacgaac ttcgtggcaa ttggcaactt ataggaagca
    46081 agcttcaatg cctgcttggc gacgtcggga gccactccac ccatctcata catgatccgt
    46141 ccaggcttca ccacggctac ccaatactcg ggattgccct tccctttacc catgcgggtt
    46201 tcagcgggct tcttcgtaat gggcttgtcc gggaaaattc tagtccatac ctggccgcca
    46261 cgtttcacaa aacgggtaat agcaatacgg gccgcctcga tttgtcggct ggtaatccag
    46321 ccaggctcaa gggcttttaa gccgaactct ccgagggtaa tggctccgcc acgataggcc
    46381 ttcccccgca tccggccctt ctgcatcttt ctgaacttaa ctttctttgg cgctaacaca
    46441 gacccacctt ttccttatcg aagttcttag ccaagtcgtc cgagcgtaga atcaggcttt
    46501 aactgaagcg ctggcaacaa ctcaccctta tagatccaag tcttcacccc gatctggccc
    46561 atcgtcgtat gagcttccgc aaacccataa tcaacctcgg cgcgcaaagt atgtagagga
    46621 acacgaccct cgcgatacca ttccgttcgg gcaatttccg ctccgcccaa acgtcccgcc
    46681 accatgatct tgattccctg cgcaccaagc cgaagcgccg actgcacgct acgcttcatc
    46741 gcccgtcgga acgcgacacg cttctccaac tgagtcgcga cattctcgct caccaattgg
    46801 gcgtccaact cgggcttttt aatttcttta acggtaatgt aggcctgacc ggaatattcc
    46861 ttctcgaggg cagccttcaa cttatcaact tcagcgccct ttcggccgat gatgatgcca
    46921 gggcgggcgg tgtgaatgat cacgcgggtc tgatccccag atcgttcaat ttccaccttg
    46981 gccactccgg cgtgatacag cttggctttc accatctttc gaatcttgat gtcttggtga
    47041 agcagtcgcg catagtcttt atcggcatac cagcgcgagc tccaggtgta gttgtagccg
    47101 atgcgatagc caattggatg tgtcttctga cccatgaaga ataaccctca gtctaatggt
    47161 aaaagcgtta gctgctgtgg gcggcgttcg gagcagctac tgcgatcgta atatggctag
    47221 tccgcttctg aatggagttg gctcgcccca tcgatcgtgc cctaaaacgc ttataaatag
    47281 gcccgcagtt caccacggcc tgcgacaccc acatctcgtc actgtcgccc aattccttct
    47341 gctcggcgtt ggccactgca gatcgaagaa gcttctcaat gactcgggcg gcgtgctttg
    47401 gagtatgccg caacatggct aaagccatgg gcacctgctg gccgcgaatc atatcgataa
    47461 ccactctcgc cttcctaggc gtcactctca cgaaacgtaa atttgctttt gcctctgcca
    47521 tagctgaccc caccccacca tgccgactca tcagtgaacc gacaattact taagcgccac
    47581 tgccttttcg gtctttgctt gtccgtgccc tttgaaaaag cgcgttggag caaattctcc
    47641 cagcttatgg ccgaccatat tttcagtcac aaacacggga atgaacttct tcccgttatg
    47701 aacagcgaac gtgtgtccga tcatgtcagg aatgaccgtt gatcggcgcg accaggtctt
    47761 gattaacttg cggtccttcg tctgattcat ctgttcaact ttcttgatca gatggtcatc
    47821 gacaaatggg cctttcgtaa ttgaacgagg cattatccgc tcctactttt tcctacgcgc
    47881 gatgataaac ttatccgtcg acttattctt ccgggtctta tagccctttg ccggagtacc
    47941 ccatggggac actggatgtg ggttaccttg acctgatttt ccctcaccgc ctccatgcgg
    48001 gtgatcaaca ggattcatca ccacaccgcg aacatgggga cgcttcccct tccatcgcgt
    48061 acgccccgcc ttcccgacaa cgacgttttc atgatccacg ttcccgacct gaccgaccgt
    48121 ggccatgcaa gtggagagca ctttgcgcat ctctccagac ttcagacgaa tctgaacata
    48181 cgccccatcc cgacccatca cctgggcgga gccgccggcg ctgcggatca attgaccacc
    48241 tttaccaggc ttcagctcga tgttgtgaat ggtggtaccc agaggcatat tgacgagcgg
    48301 gagagcattc cccggccgca cttctgcgcc ttcccctgac tgcaccatat cacccactga
    48361 cagacccaca ggagccaaga tataccgttt ctcaccatca agataatgca gcaatgcaat
    48421 tcttgaagag cgatttgggt cgtattcaat ggcggcgacc tttgcgggaa caccagcctt
    48481 gtcacgccga aaatcgatct ggcgatagag gcgcttgtgc cctcccccac ggaaccgaat
    48541 cgtcaaacgc ccgtcattat tccgaccacc agtccgaaga tgaaaccccg ttaaagactt
    48601 ttccggcttc ttcttcgtca gtttctcgcc ctggatggct gtcattcctc gacgcccagg
    48661 ggttgttggc ttatagactt ttaatgccat aactggatct cactctatcg agagactcgc
    48721 gcttagacgg tttcgtacag ctcgagcttc tcgccctctt tcaacgtgac aaatgccttc
    48781 ttccaatcag atcgttttcc gacaaaccgt cctaagcgct taatcttgcc gcgaatgttg
    48841 acaacattca cacgcgagac cttaaccttt aagagtgatt caacggcttg cttaatctga
    48901 attttattgg cgtccggatg aacaagaaag cccactgtgt tgctttgctc acgcaaggcc
    48961 gtaattttct cggtcagcaa tggctgaatc aatacctgat gaagatcgcc tttcatgacc
    49021 acacctcttt cactcgctcc aactcaccct gcggaatgac gagggagtgg caacgcaaca
    49081 cgtcatagac gttcagttct tcaggtcgta gcacggtcac gcttgaaaga ttcttgccag
    49141 cctgcatcac atgagagttc ggatcggcga caacgagcaa tgcagtacca gaaattccga
    49201 gttgagccaa tacatttgcc agcaattttg tctttgcctc tgcgatagac agctcagaga
    49261 ctacaacaac gttgccctca gacaacttgg ccgagaggac actctgaagg gcagcccgat
    49321 acttcttctt tggcattcca aacgcgtagc ttcgcggctt tggaccaaaa acggtaccgc
    49381 catgtcgcca gacaggggac cgtaaagaac cggctcgagc acggccagtg tgcttttgct
    49441 tccaaggctt ctttcccgat ccgctcacct caccgcgacg caaagtggaa gcagtgccct
    49501 gacgatcgca ggccctctgc attaccacgg cttcgtgcac aagcgaacca tgcggcttgc
    49561 acccgaatac ttcgtccggc agatctacac taccgacttt cttctttttc gaatcaacca
    49621 catcaacgat cggcatagct caactctttt ttgacttccg cacaactagc agcccattcg
    49681 caccaccagg cactgctccg cggacgaaca acaggttttc atctggacgg gcgtcaatca
    49741 ccttcagacg ctgaaccgtg acacgctcat cccccatatg gccaggcaag gccttatttt
    49801 tccagacacg agatggatag gagctcgcgc cgatcgaacc aggagcacga tggaacatgg
    49861 aaccatgcgt ctctggacca ccggcatagt tgtgtcgtct cacgacacct tgaaagccct
    49921 tacccttcga aacaccaaca acatcaaccc aatcaccctt cttaaacata tcgaccttca
    49981 cggtcgcgcc gactgcgact tccccagttt tcgggaattc acgcagccac cggctgggag
    50041 gagcctgatg cttcttcaaa tgtcccagtt caccgcctga gagtctggac tccttcactt
    50101 caccgaatga cagctgaacg gcttcgtaac cgtcgcgctc cttggtctta atggcaacca
    50161 ctcggcacgg accagcctca atgaccgtca ctggctccag aacgctctca tcataaattt
    50221 gggtcatccc caacttttta cctaacaatc cgtttgtcat ggtcttcctg tcagctcaaa
    50281 aaaggtcgag actttaaacc ctcagagctt aatctccaca tctacaccag ccgccaaatt
    50341 gagcttcatc aatgagtcca tggtctccgg cgtaggctcc atgatatcca acagacgctt
    50401 gtgcgtacga atctcaaatt gttcgcggga cttcttatcc acatgagtcg aacgctgcac
    50461 ggtgaatttc tcaattcgag tcggcaatgg gataggcccg acaacccgcg caccgctacg
    50521 cctgaccgtc tccacaatct ccaccaccga ctgatcaagc acgcgataat caaaaccacg
    50581 caacctgata cgaattcttt gatcgacttt cacgctcaac tcctcacatg ccggccaaca
    50641 gaccaggaca tcctaagcta gaatttccgt aacgacgccc gagccgacag tcttgccgcc
    50701 ttcgcgg
//
LOCUS       DAOQDS010000027        50457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_215_length_50457_cov_22.522201, whole genome shotgun sequence.
ACCESSION   DAOQDS010000027 DAOQDS010000000
VERSION     DAOQDS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 50457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50457
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215_length_50457_cov_22.522201"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /locus_tag="PK589_08510"
     CDS             complement(<1..1681)
                     /locus_tag="PK589_08510"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNJ19682.1"
                     /translation="MGKADKIIYTKTDEAPMLATYSFLPIINAFTKAAGVTVELRDIS
                     LAGRVIAVFPEYLTAEQKQHDALAELGEMAKTPEANIIKLPNISASIPQLVATIKELQ
                     SQGYKLPDYPENPKDDKEKDIKSRYDKVKGSAVNPVLREGNSDRRAPLSVKAYARKHP
                     HKMSPWSPDSKTHVSHMKGGDFRSNEKSMTIPAATTAKIEFVGADGKTTVLKEKVALQ
                     AGEVLDATFMSVKALRTFLEEQIEDAKKQGVLWSLHMKATMMKVSDPKIFGHGVTVYY
                     KDVFEKHAETFKKLGVDPDNGLGDVYAKIKSLPDDQRKAIEADIQAVYQKRPPMAMVN
                     SDKGITNLHVPSDIIIDASMPPVIRDSGKMWGPDGKLADVKCVIPDASYAPIYHEVVE
                     FCKQKGQFDPRTMGTIPNVGLMAQAAEEYGSHDKTFKAPANGTIRIVDANGTVLHQHQ
                     VEAGDIWRACQVKDAPIQDWVKLAVTRARATGAPAVFWLDKTRAHDAELIKKVNAYLP
                     KHDTNGLEIKIMSPAEACRFSIERMKEGKDTISVTGNVLRDYLTDLFPILEI"
     gene            complement(1707..3950)
                     /locus_tag="PK589_08515"
     CDS             complement(1707..3950)
                     /locus_tag="PK589_08515"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNJ19683.1"
                     /translation="MSMDLAKALYAKMPEVFAKARKKFGRGLTLAEKILVSHADNFDS
                     QNWERGKAMLALRPDRVAMQDATAQMAVLQFMQANKKKAAVPSTIHCDHLIRAEMGSE
                     KDLLRALDENKEVYNFLASAAKKYGIGFWKPGAGIIHQVVLENYAFPGCLIIGTDSHT
                     PNGGGLGGLAIGVGGADAGEVMAGLPWEVLHPKLIGVRLTGKLNGWASPKDVILYLCG
                     LLTVKGGTNKIVEYFGPGAETISATGKGTICNMGAELGATTSVFPFDEKMVAYMKITD
                     RADLATFTQSHKDLLVADPEVAQSPEKYYDQIVEIDLSKLEPHVVGPHTPDLARPISK
                     LKAEAQEKGYPVELKAALIGSCTNSSYEDISRSAHVAQQALKAGLKAKASFLISPGSE
                     RIYHTMKRDGFMDTFEKLGGTVLSNSCGPCIGQWKRSDGVKGRADSIVSSFNRNFPGR
                     NDGISETLSFLASPEVVTAYAITGDLGFDPVNQSVKGADGKEFKLQPPVGEELPAKGF
                     AKGEEGFVAPAEDGSNLTVDIPPTSERLQLLQPFPKWDGKDFEKLPLLIKTKGKTTTD
                     HISPAGPWLKFRGHLDKISDNMFLGANNSFSSEPGKGTNVLTGESNLTMAQIARAYKA
                     KGIGSIVVGDENYGEGSSREHAAMSPRFLNVRAVITKSFARIHETNLKKQGILPLTFA
                     DPKDYEKIEQNDRISVVDLANLAPGKPVTVVIHKTGGDLRIQTNHSMTAQQITWFKAG
                     SALNALN"
     gene            complement(4058..5878)
                     /locus_tag="PK589_08520"
     CDS             complement(4058..5878)
                     /locus_tag="PK589_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNJ19684.1"
                     /translation="MSILANKDTRVVIQGGQAGVNAARRMAEFCYLIKRPLNVEAFVY
                     PPDAGKTNEIPYGSGLIAIPVYKSIAEATKNHPTLNTSLVYIGADRAMKGGMEALDDS
                     HIKVVSMITEGVPEKDAKILGAHARKLGKVFNGPSSIGIISAGACRLGVIGGAFDNLV
                     LSKLYREGSFGVITKSGGLSNEIIWICSQFADGITTAIGIGGDAYPGTDYVSYLEMFE
                     NDPQTKAVVIVGEMGGDLEERAAEWYGAKKRRVKLIAVVSGFCQESLPKGMKFGHAGA
                     KEGMKGEGSARSKSDALKKAGALVPPTFGALGPAIKETYEDLLKSGQVKAPVEPAVLP
                     RLPKTVEEAMKADEVMVAPLIRTTISDDRGDEPCYDGYPASELINKGYEIPHVIGLLW
                     DKRLISKQEAEIIKRIMMLSADHGPCVSGAYATILAACAGIGLSQSVAAGMIMIGPRF
                     GGAVTDAGRFFKYAVDNKMSVDEFLAYMKKNHGPVPGIGHRVKSLRNPDKRVKELVGY
                     VKSLKIKTPCLDFALEVEKITSVKKDNLILNVDGTMAAVLVDIGFPVDSLNGFFILSR
                     TIGLIGHWVDQKRQDSRLIRLFDYLVNYAAPKRREVPPLK"
     gene            complement(5921..7120)
                     /locus_tag="PK589_08525"
     CDS             complement(5921..7120)
                     /locus_tag="PK589_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HNJ19685.1"
                     /translation="MAKVLEGPGMGLMKKWGINVPNYVVVTSVDELTKLGQANEWLKK
                     SKLVAKAHEALGSRFKLGLVKVDLDFKAAEAATKEMIGRQVGSITVTQVIVSEMIPHK
                     EEYYCAVKSTREGSEILVANCGGIEVESNWERVKRLCLDIGQSPSPESLEKLSKEAGF
                     SGALAKKMAEFAGKMFACFDSEDAQYLEVNPVVLRAGDDELVALDAVTLLDGDAKFRH
                     PDWNFAFAAEFGRAYSKQELEVMAVDSKIKGSVKFIEIPGGDTAMLPAGGGASVYYSD
                     AVVARGGKLANYAEYSGDPPDWAVEVLTEKVCSLPGIRNIIVGGAIANFTDVKKTFGG
                     IINGFRKAKSEGKLKNVKIWVRRGGPREKEGLDAMRALKDEGFDINVFDRNTPLTDIV
                     DKALQAK"
     gene            complement(7329..8174)
                     /gene="mutM"
                     /locus_tag="PK589_08530"
     CDS             complement(7329..8174)
                     /gene="mutM"
                     /locus_tag="PK589_08530"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNJ19686.1"
                     /translation="MPELPEAEVAARQLRERVVGALVSDCWVGREDIVREGLSSLEWF
                     RGATITGVERRGKSVILAFIRAAEVKFMTAELGMTGLLLFRNTPTKHPQHTHFVLKLE
                     GGAESEIRYWNPRRFGRLSLLDQAGLDRYTSRRFGPDPLVISQKQFVQLLLSTRSRLK
                     PLLMHQQAIAGIGNIYANEMLFRARLHPDQIANQVNQKAAIRLHEIMGDVLREAIDLG
                     GSSVRDYFAPDGTEGQYKRRHLVYAKSGEPCPNDCGAVIRRSIGERSSFYCPRCQRIR
                     RPQVG"
     gene            complement(8349..8771)
                     /locus_tag="PK589_08535"
     CDS             complement(8349..8771)
                     /locus_tag="PK589_08535"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HNJ19687.1"
                     /translation="MINKRLLVGNADDAKNPPPQVGAILMVAEEQNVTVPARIIYAKI
                     PLKEFGEPAVSALYDAVQWIEKHLPDNRLMVCCRAGMGRSVSVVIAYLCCVEGMTYSD
                     ALKLALTRRPGGMPLPRLDKVIEDVFRRRCAVERPSLS"
     gene            8904..9263
                     /locus_tag="PK589_08540"
     CDS             8904..9263
                     /locus_tag="PK589_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19688.1"
                     /translation="MALIQLEQVEKRLSSVQCAICKTNTFGIDRRTLQSDGECRGVCL
                     KCRYSFPVYTDMEFYLRTQPDIPYRLKEISCPNCQHRGVSLDFRITMSVREAIYFVTC
                     SACHTQFPERSSLEAFE"
     gene            9300..9485
                     /locus_tag="PK589_08545"
     CDS             9300..9485
                     /locus_tag="PK589_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19689.1"
                     /translation="MTDTQKPKPPLPVADVAPKPRKEIPLVSVPNDRDMIAEEMAALF
                     DEDRVSHQHGSSEPEAD"
     gene            complement(9490..10695)
                     /locus_tag="PK589_08550"
     CDS             complement(9490..10695)
                     /locus_tag="PK589_08550"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HNJ19690.1"
                     /translation="MYQLSSAMLGTIATVARVNNSLQNPALSASLVSGVKNLSEMFTR
                     GTKAGATAYLEDGALRASYLHYYVPVNVGKIQMLLEELRPLLPSRTNEEFRVLDVGGG
                     PGTGSLALLDWCSSQSLPPISSLRMTVVDRSIEAMKLSTRLWEEYARQINIPSIQPLQ
                     TAVCDLEHSFPPVLRMGAEKNAYHLIIVQNVLSELFAEKSDCIARRTTLINHLVDCLA
                     KDGSLMLIEPASRAESRALHQVRDNVLSELQCSIYSPCLHDQLCPALLKPDDWCHEER
                     EWQTPSWISQIDREVGFIKDALKFSYVIIRKDGKSLVPRKQDYHRVVSELREMKGEKR
                     VWLCDQTGRSEVGRQDKERSTTNATFDNWHRGAIVRVDEIVRKERKGRPAMVGRILSS
                     GVAEVIRPA"
     gene            10764..11012
                     /locus_tag="PK589_08555"
     CDS             10764..11012
                     /locus_tag="PK589_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19691.1"
                     /translation="MTDPILQAYREVEMAMERFTLVLHDHVDYLRKTEAPGSDKLHRM
                     ASGTKAMRDSASIYLSYAKYVAHGMPESPDLVEEDLQG"
     gene            complement(11097..12320)
                     /locus_tag="PK589_08560"
     CDS             complement(11097..12320)
                     /locus_tag="PK589_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ19692.1"
                     /translation="MTPEYMFLEAPRTKEFITGSEAAKEAIRRSNVDLAIAYPITPQS
                     ETMQLVGVLYGEGYVKEYYRGEEEVGVMAAIAGGSRAGVRCYTATAGPGTLRGLEGIA
                     SWPGHRLPVVAMFTCRVVNAPLAIQPDNIEVSYLLNCGMIVFHAENQQDMYDFTLAGF
                     VISEKNDVTLPVGVCCDGFFVTHARGYVRMQDRGMKLPPREPWRGAVPVLDAENPPAR
                     LSRDAPVQKSNFMAYNIHAVWQQEVWAAVERSRKYINQYMGGLLTAENVEDAEAIIIA
                     SGSAAAQSREAVRICAEKGMKIGLIKVRSLRPFPTQELRKLCGKAKLIVVPEFNYVGW
                     LAKEVATAIYGFSNAKIIGGPRVYGGQSMPVELIVDEVESGLTGKKSTNVAISQVMGA
                     SAADHEAMGHFMRSI"
     gene            complement(12469..13233)
                     /locus_tag="PK589_08565"
     CDS             complement(12469..13233)
                     /locus_tag="PK589_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HNJ19693.1"
                     /translation="MGTTQDTRERIIVPGPAGFHPPSAAQMGVSLPDPGQGLYYGLLE
                     PNEDTVIEEMARKMLTSPNATIFPGPLVLWAWNNHAVEKAKAVLEIAAQIPDVMIIPM
                     PDYRPKYPKIDPEEVINPNHPNLTIWGNKIEACIFIGVHCHYANLTLKMIRAGTNCCT
                     MAICAEQGHEDAMLTIRDSDTLKLKKTAQIFKKVREELGIKLPDNGENVRFTGTQSKV
                     HDGKTHTNPMTFMPSAAGAGSAAAFGHSPDQMKREG"
     gene            complement(13255..13578)
                     /locus_tag="PK589_08570"
     CDS             complement(13255..13578)
                     /locus_tag="PK589_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19694.1"
                     /translation="MYLVADISVEICAAKSCKLCTQYCPEANTILYSDEMGKDKGFKY
                     GSAYVAVDRCKGCAQCVWVCDNMAKNNAIKMIMIDQLPVAALTDNITYGEKSTTATLV
                     SPVVG"
     gene            complement(13604..14317)
                     /locus_tag="PK589_08575"
     CDS             complement(13604..14317)
                     /locus_tag="PK589_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNJ19695.1"
                     /translation="MATRFNIRMAGVGGQGVVTGSHILSTAVIHAGGESTIVPFYGSE
                     KRMAPVESYVRVSDEPIYEIGEITFPHIIIIFHPQVITHGKSYTMPFYFGLKENGIAL
                     INNDGPMKLHRDQARELEERHAKLYYFPATKLSLEVAGMDLATNMALMGCIGAITGLT
                     TVEALEQSVKDRFLGKGFVVSGGTAALDSVVERKFKKKQELIEKNVAVIRAGWNYAVD
                     HGWAAPSVKRSEATESVTA"
     gene            complement(14341..15240)
                     /locus_tag="PK589_08580"
     CDS             complement(14341..15240)
                     /locus_tag="PK589_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNJ19696.1"
                     /translation="MSLDYVKFSSGFEKFMPKEYRDMVEHGPFGKKVSVSQMGSFKEV
                     LEEHPMCAGCAMTLFIRLAMIAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRLRFGDKPKDVVVMAGDGGTADIGFQQVLHSWFRKERFTTIMLDNEVY
                     GNTGGQESGMTNRGAVLKMAPLGKKFEKMDMLQMAKVAGCAYVATVVPNNPRRVESVI
                     KKAVLIAREVGSTYIQAYTSCNIEYAIPTDKVMEDAKNVENDRYQFSEYITDEAKQFL
                     TERYGYKEFLPKPAAPAPGLPKA"
     gene            15981..16409
                     /gene="queF"
                     /locus_tag="PK589_08585"
     CDS             15981..16409
                     /gene="queF"
                     /locus_tag="PK589_08585"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNJ19697.1"
                     /translation="MREQNKKKLKTGNSTTKLGYNERHARSGISAPLPDIETFPNQYK
                     GYEITIVIPEYTAICPKTNLPDFGTITLHYKPNKQCLELKALKMYVHAYRNIGIFYEN
                     AVNRILQDVVRACRPTWAKVTGEFAARGGLRSVIEAKYPE"
     gene            16716..18017
                     /locus_tag="PK589_08590"
     CDS             16716..18017
                     /locus_tag="PK589_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19698.1"
                     /translation="MSVPTADAIPSFARKYALDCTACHTAPPRLNTFGERFLENGYQL
                     PGTTDGGLTGKKNLGDVSLDDVANYTGVRLRGNVLRTHHFKQQNPSGAEAGTVQNKSD
                     LAFPEVFSLFTAGTLAKNIGFFAELESNLEASHTGIERAFLTFNNIGGENVANIRVGK
                     IDPSAAFSFSTLRQQLDFIAESTHPSTNAVQRAGLFPLATAAKFYGLRNRSGGTISPY
                     APSLYNAVVETGAEVRGRPFGDWFMYQLGVLNGANEGFGDSNKGKDFYGAIRFDYARS
                     ANFSASLSGFAYLGNSNATVFDGTRTTDVNWSRYGVAAHLRYKMFDLHGMYTLDRITH
                     APATALSNFDTDASGLTVALDTHVANNTLFSLRYDTMDAGGDLSQRSSQSFLGAQVKH
                     YVRSNIALYARLDHNLRHAEDGQAAARNLREAFFAGIDVAY"
     gene            18042..18815
                     /locus_tag="PK589_08595"
     CDS             18042..18815
                     /locus_tag="PK589_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ19699.1"
                     /translation="MGISMYKYLVILAVSGLCATSAYAVRPEDNRDTLAEGRRIYERA
                     CAWCHGNGGLGDGPSGWSIGRYSAPRPRDFTKESYKLRSTPSGELPTDQDLFRTLTQG
                     IPGVMPSYRSLNESERWQVIAYLKSFNPAFEQETPVSIPLPPPPVSRPEGAVQHGRVL
                     YAKYDCRVCHGANGEGNGPESQAGRLRDTNNLPIRATNLTDPASFKNGSTAQDLYRSI
                     MTGLDGTPMPSYANEFAGQEEAVWDLVWYLQSLSGRPEK"
     gene            18896..19615
                     /locus_tag="PK589_08600"
     CDS             18896..19615
                     /locus_tag="PK589_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19700.1"
                     /translation="MVMRLMLVVLGLTLGVPSLLQAESPTGKLAGRITYSGSALPDQT
                     AEITRDSAFCGTSTTIKDVVIHPKTRGLEGVVVSIESGNLPSSDAPLAPLVLTNTHCA
                     FLPRTVIGQVGQQLEIRSDDPVMHNTHISLGSRTFVNVAMIPASRPVRKPLKQPGIYT
                     VKCDAHKFMHADVIAFAHPFFSQTDESGSFQITNLPPGEHVVNIWHHTLGSYQQTVRV
                     SAEAETTIAFDFAAHAESNKK"
     gene            19617..>22489
                     /locus_tag="PK589_08605"
     CDS             19617..>22489
                     /locus_tag="PK589_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ19701.1"
                     /translation="MPWFHHLWMMWQGSLRLRLSVGMAVLITLGMTVFATIRLVEIRH
                     SLVDAAQARALAVSRTFAMMGAAAVLDNLFRIQEALTRYANEADIVGIVIIDPDNMVV
                     AATKPHDIGRELTDHTLSLAQTSGSETMAHRLSEDGTPTLIVVTPLKNEDDIAAWVRI
                     EFSLAAMQGELVREVRRLLVLSVLLMSVTIVVGQIGIRRMSALFRDTAAKLQDTLHTL
                     HRPLKEDAGNATSLNGDSPGPASYTGELEQMVDLVETTTALLRTQAQRLQSFTDSLED
                     AVRQRTTELHQAKDAAETANQAKSQFLANMSHEIRTPMNGVLGMTELLLTTALTPKQR
                     SLAKTLHQSGTGLLDIINEILDFSKIEAGKLKLECIEFGLRQTVEEAIDLVAETAHRK
                     HLELTCFIPEEIPDAVVGDPVRLRQILLNLVGNAIKFTQAGDVSVTLSLVSQEASRVN
                     LKCCIRDTGVGISDEAKARLFQAFSQADGSTTRRFGGTGLGLAIVKQLAHLMGGDVGV
                     DSIPGQGSTFWFTVSMEYASQAVSAHEGLASPLAGHSILVVDDNSTNRHILEAHLSNW
                     GATVQSAASSEDGLRLLTEAKGGNQKIQMAVLDIRLPGMNGIDLARKIREDVDLRDMA
                     ILALSSEDRETDESHHHPKLFNAWLRKPVHQSLLKDCLTRLCLAPSAEDASATIQPPE
                     QTRTLAGHILLAEDNPVNREVACTMLEILGCTTAVAEQGKEAVETAAHGAFDLILMDC
                     HMPEMDGLTATGLIRAHEQQSMPARHTIIVALTANALEGDRERCLAAGMDDYLTKPFT
                     LDALKELLQRWLPEHVPSDSTSDSQPAEVSNGVPPQQAVSSHAQPLAAESEHVDAHAW
                     EAIRARQRPGQPDFLHKALSLYLPYADKQIARLENAIADQDARAIATIAHTMKSSSGQ
                     LGAQRLALLYTELESASRAGRIGDFQELYDRLIPEHRAVSALMR"
     assembly_gap    22490..22589
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22590..25486
                     /locus_tag="PK589_08610"
     CDS             <22590..25486
                     /locus_tag="PK589_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ19702.1"
                     /translation="ESEQVIARQQYESSLRESEEKFRRLFESSRDAIMILYPPNWNFS
                     ACNPATVQLFGARDEAHFTSLGPGDVSPEYQPDGELSMVKARKAIEAAMREGFHLFEW
                     MHKRVGGPTFLATVQLTRITLQGVEGLQATVRDITEQRRAETELRAYATFQRAILDHA
                     GSAIISCRPDGIIQLFNPAAEALLGYTADELIGRQHPGIFHVREEIVERARQLSEEFG
                     CTIAPGFDVFIEKSRRNLPNEHEWTYIRKDGTRRTVLLNITALRDADSKITGYLAVAS
                     DITPLKRTAQELLLAKEAAEAASRTKSQFLANMSHEIRTPMNGVLGMTELLLTTALDT
                     RQRHLTRTIQQSGEALLAIINDILDFSKIEAGKLQLERLDFDLQDTVDNAVELFATPA
                     QRKGLELTCHMPSAIPRTLRGDPIRLRQALLNLISNALKFTSTGEINIRIDAVVETDA
                     SVTLRFAVKDSGVGIPAEAHQRIFEAFSQADGTTTRRFGGTGLGLTIVKELVSLMQGQ
                     IGVESQVGQGSTFWFTAVFERQPATTGDPYPEHALLQRRILVVDDTAANREILDDHLR
                     SWGAIPALTASAAEALTQLRAAAARQQPFELAILDFQMPDMDGMQLAQAIRADPQLSS
                     LPLLMLTSVGYDAGAPGVPAVDGWVTKPVRKTLLQQAILGLFRTGQRAAIRRPDPVVS
                     EPVTTVSHSARLLLVEDTPVNREVATGMLDVLGYSVHAVENGRLALEAVAREQFDLIL
                     MDCQMPEMDGFTATAAIRQQERQSGGGHRIPVIALTANAMDGDRTRCLAAGMDDYLAK
                     PFTAAQLRTILTHWLTPPVADATTASMPTQTAPPRESTDESPQPTANPEIDKTAWEAI
                     RSLQRPGRPDILAKVLTTYLNDSRILVEEMRAAVEARDPGTLAKAAHRLKSSSAQLGV
                     LATAAHCKELEHLGRLASLNDAAHLLAQLAETHQAACTSIAAELRSRSST"
     gene            25632..27734
                     /locus_tag="PK589_08615"
     CDS             25632..27734
                     /locus_tag="PK589_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ19703.1"
                     /translation="MRCLARTYNWMLPTTTGCLLAATILGTPLTSLAASPIEDAPLVQ
                     TEASLDLPDLPSSGTPDGQEPVGKESTLFEEIPSVYGAAKYDQKVNEAPAAVIIITAD
                     QIKKYGYRNFAQILDSVPGLFTSNDRNYGYLGIRGFNRPGDYTSRVLLLIDNHRLNDA
                     VYDQGGAGTEMPIDVDLIDRVEIIKGPGSALYGTNAFFGVVNVITKRGRDLKGAEVSG
                     EASSFNTYRSRLSYGNKFSNGLEMLLSGSFSDSEGHRQLSYPAFSSINNGIAKDADRD
                     NLYYYTGKLSYGDFTLLGGHQWRKKNVPTASFGTVFNDNRQNTLDERTYLDLVYQHEF
                     VNQLNVKARVYYDRYYYRAHLPYDYAGTGTPPFTLNKDSTEADWWGAELTITKRLFDH
                     HKFTIGSEYRDQFRLNQANADIAPPSTYLDDKRRSSFIAAYMQDEWAITDHLVLTAGL
                     RHDQYSTFGGTTNPRAGLISTWDKTTVKLLYGQAFRAPNPYEQFYVTGSTFKANADIK
                     PERIKTYELVVEQYLGDHLRALGSLYQYNISGLISQTTDPTDGKLFYSNVDNITARGV
                     ELAMEGKWPSGLEGRLSYALQRTENDDLKQQLTNSPQHLAKANVIVPVIADKLFAGFE
                     GRYTSSRLTLAGNSASPFYILNMSLFSQRIVKGWELSGHINNLMNQKYGYPGSGEHVQ
                     DVIDQDGRTFWVKLKYRF"
     gene            27751..28701
                     /locus_tag="PK589_08620"
     CDS             27751..28701
                     /locus_tag="PK589_08620"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ19704.1"
                     /translation="MRRLIHLIAALGIALTLSALWLIASPDSACAESGRIVVLSGQDL
                     KPYQDVLAGLQQSLTKQGINLAVEIHAAQGAPSTANVLEDIKKNGARLVVTLGSAATQ
                     AAVREVGQVPILATMIVTADDIKSASNATAVLLDFPLDLQMQWLHRIVPAANTVGVLF
                     NPKENQTKVNHALRIAKENGLSLVTQAVDTPRALPDALENLSRHVDALWGISDSVVMT
                     PQTAEPILLSTLRNKVPLAGLSASWVKAGALYALDRDYLDIGNQCGDIAGKILGGTSA
                     SSLAPIFPRKVTYSVNLKTAGAMNLELPQDVVRGATHIFQ"
     gene            28719..31622
                     /locus_tag="PK589_08625"
     CDS             28719..31622
                     /locus_tag="PK589_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ19705.1"
                     /translation="MIKKGIGLRDKLNLLSIGLVVLTAIGIATFVVHREITTRYHDLV
                     NTGLTTAAMIAQNSEYAVYTENQDAVRRAVGGLHALPDIAYAAVLGKERLPILEETWG
                     ANIAIPTALRTRLLAAGDALFEEVAIQQGHPAYIDILVPVVSRPAAAADPLFLESNAS
                     SPPGAVIGYIQLGLSQERLRKNLQEFVLITGLVIGVVLLVGLCMSLILTRSITKPILA
                     LVEAAGHIAEGRLDVDVAAGTHDEVDRLADAFNRMTGRLRVSQSQVQEYQQSLEDKVT
                     QRTKQLELASQEAQRLAEEAQAASRAKSQFLANMSHEIRTPMNGVLGMTELLLSTTLD
                     TRQRHLTKTIQQSGEALLAIINDILDFSKIEAGKLQLEQLDFDVQDTVENAVELFAGP
                     AQRKQIELTCQLMGPFTHALRGDPIRLRQALLNLISNALKFTAKGEINVRVYAVTETN
                     TTVTLRFEVKDSGVGIPAEAHQRIFEAFSQADGTTTRRFGGTGLGLTIVKELVALMQG
                     QIGVESQVGQGSTFWFTAVFERQPAAAPGQEQAPEQALSKKRILVVDDTPANREILYE
                     HLRTWGALPTLAASAQEALVLLGTGSNSPQPFDLAILDLHMPDMDGLELAKAIRMDPR
                     LAGLPLLMLTSVGYDAKTPGTPDLDAWVTKPVRNTLLRQALLGLLHTRHRAPVQPPAP
                     LPSASGPIPFQAAHLLLVEDTPVNREVALGMLDMLGHFAQAVENGRLAVEAVARERFD
                     LVLMDCQMPEMDGFTATATIRQQERDAADHRHVPIIALTANAMEGDRARCLAAGMDDY
                     LAKPFTMAQLSEMLTRWLTLQATAAPMAPGPSPSARQDDSPSDQTPPSAPVEIDRTAW
                     DAILALQRPGRPDILAKVLTAYLNDSRTLVGEIRTAVQTQDAVALAKAAHRLKSSSAQ
                     LGALATAAHCKELENLGRLARLDDAPSLLAQLTDAHQAACAAMTSELLQRPAA"
     gene            31689..32507
                     /locus_tag="PK589_08630"
     CDS             31689..32507
                     /locus_tag="PK589_08630"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HNJ19706.1"
                     /translation="MATYAIGDVQGCFSSVTRLTKTIQFDPKQDRLWFVGDLVNRGPD
                     SLKVLRYIRALGQAAITVLGNHDIFLLAVAAGLTSPRPSDTLSAILEAPDCRELMLWL
                     RQQPLLYREGDFLLVHAGLLPAWTVEQAETLAGEAHNAIRGDQFLTTLRALHPSGSLQ
                     WRPDLTGPVRVATIIKVLTRLRACSDDGIMESSFSGPPDQTPDGFYPWFDVRGRRSTA
                     ATIVFGHWAAMGLRLTPNMLALDSGCVYGRQLTAVRLEDRKVFQVGCEETRGGK"
     gene            32494..33027
                     /locus_tag="PK589_08635"
     CDS             32494..33027
                     /locus_tag="PK589_08635"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HNJ19707.1"
                     /translation="MAESDQPVLALWPRYTDRPLPAYRFIPGRTPHPRRHPQGHSFEQ
                     PDPLLRPFEPEHWKTSEDYLYGIDLYNWTYWWEAHEVFEAFWHAYGRHTPAGNFFQAL
                     IQCAAANLKRELGHEQATRNLVSRALARLQKCPVHYMGLDTEIIAEQFTQWLNQREPH
                     GHVLFALSKAGGKTRNS"
     gene            complement(33046..33834)
                     /gene="panB"
                     /locus_tag="PK589_08640"
     CDS             complement(33046..33834)
                     /gene="panB"
                     /locus_tag="PK589_08640"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNJ19708.1"
                     /translation="MTVPDLQKCKRDGRKIAVVTAYDALFARIVEQAGIDVILVGDSL
                     GVVVQGKANTLSVTMDEMLYHTKLVAGAVQRSLVIGDMPFLSYQVNQEEAVRNAGRLL
                     QVGAHAVKLEGGAAMTDRVRAMANVGIPVIGHLGMTPQSVQQYGGYRVQGKGKDRAQQ
                     LLEDARALEEAGAAAIVLEAIPAGLAKTVSEALTIPTIGIGAGPHCDGQVLVLYDLLG
                     LFDEFVPKFVKPYGHLKVEALQALRQYKEDVEKGVFPSDSESYH"
     gene            complement(33834..34724)
                     /locus_tag="PK589_08645"
     CDS             complement(33834..34724)
                     /locus_tag="PK589_08645"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNJ19709.1"
                     /translation="MSREPRRLKDVLAQGQFAVTVEYNPPKGTNITHVVESAKSLVGR
                     VHGVNVTDNTAAIVRAGSLPVCRILYELGHDPVMQLTCRDRNRIAMQSDLMGAHMLGI
                     RNILCLTGDYPTVGDHKDAKPVYDLDSVQVMQLVTGLNNGKDYAGNKLDGSTDFTIGG
                     AVTPEADPLGPMLVKFEVKVRAGAQFFQTQAIYQPEQFKKFMDAVRPFKVKVLAGILL
                     LRNAKMAEFMNANIPGVCVPQDMIDEMRAAGDKRALDAGVEIAVRTIKAVRPYCDGVH
                     IMAIKSTERLPEILTKAELG"
     gene            complement(34882..35742)
                     /gene="folD"
                     /locus_tag="PK589_08650"
     CDS             complement(34882..35742)
                     /gene="folD"
                     /locus_tag="PK589_08650"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNJ19710.1"
                     /translation="MAARIIDGKALAQQVRERLAVDSAAVLAKTGTKPGLATILVGDD
                     PASHVYVKNKQKACELAGIYVDDHKLPASTTQAELLALIEKKNADPKIHGILVQLPLP
                     KHIESRVVLEAVSPNKDADGFHPYNFGRLVEGNPVFEACTPKGVIKMIESAGVSIEGK
                     RAVVVGRSNIVGKPLALMLLQRNATVTICHSKTKDLAAVCREADLLLVAIGKAKFVTA
                     DMVREGAVVIDVGTNKGPDGKLCGDVDYEPVSQKAGWISPVPGGVGPMTIAMLLDNTV
                     ESAKRMAGMK"
     gene            36005..37027
                     /locus_tag="PK589_08655"
     CDS             36005..37027
                     /locus_tag="PK589_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19711.1"
                     /translation="MTIFSFSSTRPRGRRNLGLTLAGAMGMVLASSWAWALQVSGLES
                     PHSFLADPPSRSYFISNINGDSNARDNNGFITKLSDDGRITAFKFIEGGRGEVTLHAP
                     KGMAVVDQVLYVTDVDTLRAFDKNTGKPVATVMLPRPADNKTTQSLVDVAADGKGHLY
                     LADQGGNAIYRVDLTSPLTLSLYVSGAHLAGPSGVAVNPKTGHIVVVSYDSGKIFDIT
                     PEGALTELASNGFFTGRFQNLSGVDFDRWGSMYVSDATKGKIWRMLPNNKFQVIAEYL
                     PGPSDLGIDRVNHLILVPYQDSNAAEVNGLESPTVSSGDRAKRTLADYGFGEPLKADK
                     EGSARK"
     gene            37024..37725
                     /locus_tag="PK589_08660"
     CDS             37024..37725
                     /locus_tag="PK589_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19712.1"
                     /translation="MSQCVYCKQRKGKRSCPALTGLICSQCCGEHRLARISCPPDCEY
                     LDAGSDYQQKRLGEQFAPVRRELYRQLSVAGGEKAAALFNLIEVVTFGYFHDRRDGQD
                     AEVFAAIQALRRTLSPLHVPAAPMPVFAERLKKEYDTFVKQQPQQIADAGSAPEILDR
                     ALALLTEFSGQGFQSRRFLLGMIGYVKTFHPEIAEHLTKQHEAGRIVLPGQLTPPPQE
                     PAPHVHSPECRHHHH"
     gene            37917..39656
                     /locus_tag="PK589_08665"
     CDS             37917..39656
                     /locus_tag="PK589_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ19713.1"
                     /translation="MHSFVSNLLAALPKGDALDDPAWSVRHRGILYILWLHVLGVPLY
                     GAYMGAGPSLYLGGGALLGAIAIAAQLPAVGRRLQAAIATYGLVTASALLVHLSSGRI
                     ELHFHFFVMMSVIVLYQDWLPFLVGLQFIVIDHGILGTLVPSMVYVHAEGQAHPWTWA
                     LIHGSFILAQCAALLYFWRVNEVAREEALQSEARTRMIIETALDAVVTTNVSGIITDW
                     NTQAELMFDVSRTDAIGQPLTTYLSTAHSSTAADAGVVLSGLVPGAILNRRVESIGRT
                     GSGQEFPVEIATSCLAIGGTQQFTTFIQNISERKQHEEALCRAKEAAESASQAKSQFL
                     ANMSHEIRTPMNGVLGMTELLLATQLNDRQRRYADTAHTSGTNLLHIINDILDFSKIE
                     AGRLVLEHIPYDLRQLLAETATIFHEQAGKKGLSLTLAIAQDIPMTLHGDPHRLRQIL
                     TNLISNALKFTTTGRIAISAAMDQARPEHVRIEVSDTGIGIPEDAQARIFDSFAQADG
                     STTRKYGGTGLGLAIVKQLVGMMGGHIEVTSVPGEGATFWFTILAYATAARSTASDPS
                     TSSTEALRRLHHK"
     gene            39669..40817
                     /locus_tag="PK589_08670"
     CDS             39669..40817
                     /locus_tag="PK589_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNJ19714.1"
                     /translation="MHVATTSLKAGHAGIPAELAAHDSENSLLLLFGSSSLIDRPELI
                     QQVMAACPRSHVMGCSTAGEIHGVEISDDSLVVAAVRFEKTRLLTTQAAVHTPKDSYA
                     AGRAIAASLHQPSLRGVLVLSDGLNVNGSELVKGLNDTLGGSVVVTGGLAGDGTDFKR
                     TWVIKDRAPQSGYVTAIGFYGDHVRLGHGSKGGWDKFGPERRVTKSAGNILYELDGRP
                     ALQLYKEYLGDRAAGLPATGLLFPLAIRSSNTDGKVLVRTILAVDEAAQSMTFAGDLP
                     EGMLAQLMRANFDRLIQGASEAATLTLNNHEPAGSSSPTLSIAISCVGRRLVLGERTE
                     EEIEATLEILPNGSRQVGFYSYGEISPYASGACDLHNQTMTLTTITEL"
     gene            40823..>42627
                     /locus_tag="PK589_08675"
     CDS             40823..>42627
                     /locus_tag="PK589_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ19715.1"
                     /translation="MHPLLTRQLKRLGLETTAAPTSLEAWQQLLERVSRSYIESDQGR
                     ELLERSIALSASEMQELNEQLRRTSESQLTQERDKLHTVLRSMGDGLCVVDRNWDIVL
                     LNPEGQRLWGLPEADVVGRRLPDMISLSYGTNLTEPIFTQMLLDETAQGHSFRTDDGI
                     LTSITGRSFPVSCVLAPIVRDGQAGGAVLVFRDISERKQAEDVRRHTESLLRQHQAAL
                     FGLTSNSIIQSGVLEPALREITRVTAATLGVRRCSIWLLRDDHSAIICKDLHDASNNS
                     HSCGMELLAAQFPKYFRELLTERVIDASDARTDPRTSEFTDTYLTPLGISSLLDIPIR
                     FKGKLVGVLCNEHVGTPRSWMLEEQQFSYAVASLVSLALEAVERLHAEGALRKSEGRT
                     RLIIDTALSSVISIDEQGRIIGWNTQAEQTFGWTRQEAIGRLMAETIIPASQREAHQR
                     GIRQFLKTGEGPILNKRIEISALRRDGTEFPIELAITPLRLENAYTFTAFVVDISERK
                     QAEEALRTSEARLTMTVQGSHIGIWDWNLTTDAVYFSPQWKSQLGHDDTTLPNAFQEW
                     QSRIHPEDRALVHQTVHSCLNGDRAQFELEHRLRHR"
     assembly_gap    42628..42727
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <42728..44284
                     /locus_tag="PK589_08680"
     CDS             <42728..44284
                     /locus_tag="PK589_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ19716.1"
                     /translation="KQRHLASTVHRSGRTLLAIINDILDFSKIEAGKLDLECVGFDLS
                     QLLDESLELFMEAARRKHLHLTQQVDERVPRYLMGDPVRLRQILMNLLSNAMKFTETG
                     GVSLTADYMSGTPTHAQLRISVTDTGIGIPPAAKLRIFDAFAQADGSTTRRYGGTGLG
                     LSIAKQLVGLMGGAITVESEPGRGSTFSFTARFELQPLGTGTDNGLPSYERLAKPHLT
                     LEPPYGPSEPYPSSPGTEAKRTGHRVARILIAEDSPVNREVAVGMLEILGYQVEMAEN
                     GRQALELAGRSQFDLVLMDCQMPEMDGFTATGEIRRREAASGQRRLTIIALTANAMQG
                     DREQCLAAGMDDYLTKPYTQVQLQTVVQKWLDKQGTDRALAPVFSSTQAVSGESAPAA
                     AAPPPSTPSMDPKALDGIRALQRPNRPDVLASVLRKYLDNSRDSVDALQDAIRANDPA
                     GLQAVAHRLKSSSAQLGALAVAARCKELEALGAQKNLIEADRILVELQSEYAAACAVF
                     RNEIAKEKSS"
     gene            44281..45030
                     /locus_tag="PK589_08685"
     CDS             44281..45030
                     /locus_tag="PK589_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ19717.1"
                     /translation="MNPLTSGRSPYALVTDDDIIIRMFAREALEQAGWEVEEAENGLE
                     ACAAFKKCPPDVVLLDVMMPEMDGFQTCAALRQLPGGEHTPILIMTGLDDFESITKAY
                     DAGATDFIVKPLNAMLLTHRIRYMVRANQVLQELRASQATLTQARDAAMEGARLKSEF
                     LATMSHEIRTPMNGVLGMTDWLLETELTAEQLDCAQTIRSSGDSLMVIINDILDFSKI
                     ESGKLSLELLDLDLPKFVDRVLALFAERAQR"
     gene            45052..45528
                     /locus_tag="PK589_08690"
     CDS             45052..45528
                     /locus_tag="PK589_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ19718.1"
                     /translation="MADDVPAALCGDPTRLQQILSNLLANAIKFSDRGTVSLTVDLDQ
                     RPSEQRLEFPATSYGELSSTASRISHVRFSVADNGIGIAPNAFAKLFQPFVQADGSTT
                     RKYGGTGLGLAICKQLVELMGGHIGAESEPGAGSVFRFTVPLQQPLADNERNKSAA"
     gene            45830..46720
                     /locus_tag="PK589_08695"
     CDS             45830..46720
                     /locus_tag="PK589_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HNJ19719.1"
                     /translation="MHCLTFDIEEHFQVSRFDSPIRRRHWGAFESRVTANTCKVLDLL
                     ARYDTKATFFVLGWVAERHPGLIKQIAECGHEIASHGYGHELVTAQTPELFRADVRQA
                     KQILEDLIGSPVQGYRAPGFTITRETLWALPILAEEGYTYDSSIVPIRHDHCGLPGSD
                     PWQHLKQTSSGPIWEVPPSTVNLGGVRIPIAGGSYFRLLPFPALCGLMRRIEQKGRPL
                     VMYFHPWELDPYQPHMEGPFLSKMFHYLNLDKMEFRMSTVMNSFRFGSIAEQIDLSPI
                     LPIDPTVFPSTPAEIAQDVV"
     gene            46883..47458
                     /locus_tag="PK589_08700"
     CDS             46883..47458
                     /locus_tag="PK589_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19720.1"
                     /translation="MQKRQANLWGWWLRRVGLTVACFCVVLLPTTGFGQSIEDTVQFI
                     SDMANTHGFVRSLSCRNPKAAKPTKVTEIYSVIPTGTRLGTIELNHGHDDVNTGFTHF
                     NLQDIESIAYQGQDTRENNLVLYAVRISCNSNGPCILKTSFCTGAVTEVKGQFPEDTL
                     LFRSASHAERMAKALSHFLTLVRTQQQNQPF"
     gene            complement(47524..48177)
                     /locus_tag="PK589_08705"
     CDS             complement(47524..48177)
                     /locus_tag="PK589_08705"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ19721.1"
                     /translation="MMTQSRWHNRFGCAAVMSLVLAIGMATTGLAADAPAKAGDSVNA
                     SNVRAVIKTKVGDIEIKFLPDVAPKHVENFIKLAKSGFYNGTIFHRVIPGFMIQGGDP
                     NTKDSLRKDAYGQGGPGHNVKAEFSDLPHKRGVVSMARAQDPDSAGSQFFIVVEESRF
                     LDRKYTIFGEVVKGIGVADKIVALPRVMCQTGAPSPADKGPCDNPIDRVEMTVTIIE"
     gene            complement(48207..48914)
                     /gene="rnc"
                     /locus_tag="PK589_08710"
     CDS             complement(48207..48914)
                     /gene="rnc"
                     /locus_tag="PK589_08710"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNJ19722.1"
                     /translation="MTSATSIEAVQRLLGYRFRQPRLLEEALTHKSYSNERRTKDRTQ
                     NERLEFLGDAVLSLVMSEYLAAEFPGSNEGGLSKLKAHLVSEASLAKAARRMKLGRLL
                     RLGKGEELSKGREKHSLLADALEALIAAVYLDGGLEASRTFTLRVLEEELLAARAERA
                     RPGMDDYKTQLQELCQKRFESLPQYATVRESGPDHEKVFEVQLTIQGVMRGVGCGHSK
                     KEAEQMAAREALTQLTT"
     gene            complement(48942..50192)
                     /gene="fabF"
                     /locus_tag="PK589_08715"
     CDS             complement(48942..50192)
                     /gene="fabF"
                     /locus_tag="PK589_08715"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNJ19723.1"
                     /translation="MHDRPVRRVVVTGLGLVTPLGTGVDKTWKALCAGESGVGRITRF
                     DPSGYDAQIAAEVKDFDPAQFIEKKEIKKMDTFIHYAVGASQLAVDDAGLKVSAEEAT
                     RVGVYIGSGIGGLGSIEHYHDVLKEKGPGRVSPFFIPMTIINLASGQVAIRVGAKGPN
                     SCAVTACATGNHCIGDAYRLIKRDDADVMIAGGAEAAITPLGVAGFASAKALSFRNTE
                     PAKASRPFDKDRDGFVLGEGAGVVVLEELEHARARGARIYAEVIGYAMNSDAYHITAP
                     PEEGEGAVRCMEMAIKDAGVAKTDIGYINAHGTSTMADAIETKAIKHVFGEQAYKIPV
                     SSTKSMTGHLLGAAGGIEAVFSILALHHGILPPTINLDHPDPACDLDYVPNKARATKT
                     QVVLSNSFGFGGVNACLLFRRCDQ"
     gene            complement(50204..50437)
                     /gene="acpP"
                     /locus_tag="PK589_08720"
     CDS             complement(50204..50437)
                     /gene="acpP"
                     /locus_tag="PK589_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNJ19724.1"
                     /translation="MATVDERVKKIIAEQLGVEEEEVTLEAHFVEDLGADSLDTVELV
                     MALEEEFEIEIPDEDAEKILTVGKALDYIKEKA"
BASE COUNT        11090 a        15194 c        13190 g        10783 t
ORIGIN      
        1 cgatttcgag aatcgggaac agatcggtga gataatcgcg gagcacgtta ccggtgacgg
       61 aaatcgtgtc cttgccttcc ttcatccgct cgatggagaa gcggcaggct tcggctggcg
      121 acataatctt aatctccaga ccgttcgtgt cgtgcttcgg aagataggcg ttcactttct
      181 tgatcaattc cgcgtcatgc gcgcgggtct tgtccagcca gaacacggca ggggcgcctg
      241 ttgcgcgagc gcgcgtaacg gccaacttga cccaatcctg aatcggggca tctttaacct
      301 ggcaggcacg ccaaatatcg ccggcttcca cctgatgctg gtgcaagacc gtgccgttcg
      361 catccacgat gcggatggtg ccgttggctg gcgccttgaa ggtcttgtcg tgtgaaccgt
      421 actcttctgc cgcctgggcc atcaacccga cattcgggat cgtgcccatc gtgcgggggt
      481 cgaattggcc tttttgctta cagaactcga cgacctcgtg gtagatcggc gcgtaactgg
      541 cgtctgggat cacgcacttc acatcggcca gcttgccgtc tggcccccac atcttcccgc
      601 tgtcgcggat gacgggaggc atggatgcgt cgatgatgat gtcgctcggc acatgaagat
      661 tggtgatccc cttgtcgctg ttcaccatcg ccatcggcgg gcgcttctga tagaccgcct
      721 ggatgtcggc ttcgatcgcc ttgcgctggt cgtccggcag ggacttgatc ttcgcatata
      781 cgtcaccgag accgttgtcc ggatcgacgc cgagcttctt gaacgtctcg gcatgcttct
      841 caaatacatc cttgtagtac acggtcacgc catgaccgaa gatcttcgga tcggagacct
      901 tcatcatggt cgccttcata tgaagcgacc aaaggacacc ctgcttcttg gcatcttcga
      961 tctgctcctc caagaacgtg cgcaacgcct tgacgctcat gaacgtcgcg tcgagcactt
     1021 caccagcctg cagagcgacc ttctccttca aaacggtggt cttgccatcg gcaccgacga
     1081 actcaatctt cgccgtcgtc gcggccggaa tcgtcattga cttctcattc gaacggaagt
     1141 ctccgccttt catatgagaa acatgggtct tggaatcggg actccaggga gacatcttat
     1201 gcgggtgctt ccgggcatag gctttgacgg acaatggggc gcggcgatcg gagttgcctt
     1261 cgcgcagtac cggattgacc gcgctgcctt tgactttgtc gtagcgggac ttgatgtctt
     1321 tctctttatc gtccttagga ttctcgggat agtctgggag cttatacccc tggctctgca
     1381 gttccttgat ggtggccacg agctgcggaa tagaggcgct gatgttcggc agcttgataa
     1441 tgttggcttc gggggtcttc gccatctccc ctaactcggc gagggcgtcg tgctgtttct
     1501 gttcggcagt aaggtattcc gggaatactg caatcacgcg gcccgcaaga gaaatatcgc
     1561 gcaactcaac ggtcacgccc gccgctttcg tgaatgcatt gatgatcggt aaaaacgaat
     1621 aggtcgccag cattggcgct tcgtcggtct tcgtatagat aattttatct gcttttccca
     1681 ttgggcttct ccctttgctg tattgtttag ttcaaagcat tcaacgctga tccggccttg
     1741 aaccaggtga tttgctgagc ggtcatgctg tgattcgtct gaatcctcag atcaccacca
     1801 gtcttatgaa tgaccactgt cacgggcttg ccgggcgcca aattggccag gtccacgacg
     1861 ctgatacgat cgttttgctc gatcttttca tagtccttag gatcggcaaa tgtgagcggg
     1921 aggatgcctt gcttctttaa attggtttca tgaatgcggg caaagctttt ggtgataaca
     1981 gcccggacat tcaagaagcg tggagacatt gcggcatgtt cgcggctact gccttccccg
     2041 taattctcgt ctcccacgac aatcgatccg atgcctttgg ccttgtaggc gcgtgcaatt
     2101 tgggccatcg taagattgga ttcaccagtc agcacgttgg ttcccttacc aggctccgac
     2161 gaaaagctat tattggcgcc taaaaacata ttgtcgctga tcttgtctaa gtgaccgcgg
     2221 aacttgagcc atggccctgc cggcgaaata tgatctgtcg tagtcttacc tttcgtcttg
     2281 atcaggagcg gcaacttctc gaagtcttta ccatcccact tcgggaaagg ctgcaacaat
     2341 tgcaaccgtt cgctggtcgg cggaatatcc acggtcaggt tcgagccgtc ctctgcgggc
     2401 gctacgaatc cttcttcacc cttcgcaaat cccttggctg gtaattcctc cccaacgggc
     2461 ggctgaagct taaactcttt cccatctgcc cccttaaccg attggttgac tgggtcaaaa
     2521 cccaaatcac cagtgatggc gtaggcagtc acaacttcgg gactggcgag gaaagacagc
     2581 gtttcgctga tgccgtcgtt acgtccgggg aagttacgat tgaaggaact gacaatggag
     2641 tccgcacgac ccttgactcc atctgaccgc ttccactgcc cgatgcaggg gccgcaggaa
     2701 ttagacaaca cggttccgcc gagcttttcg aacgtatcca taaacccgtc ccgcttcatc
     2761 gtgtgataaa tgcgttcgga gccaggagag atcagaaatg aagccttcgc cttcaagcct
     2821 gccttcaatg cctgttgagc cacgtgggcc gaacggctga tgtcctcata tgaggaattc
     2881 gtacaactgc cgatcaacgc cgccttcagt tcaaccggat atcccttttc ctgcgcctca
     2941 gctttcaact ttgaaattgg ccgcgccaga tccggggtgt ggggaccgac aacgtgcggc
     3001 tccagctttg acagatcgat ctcaacaatt tgatcgtagt atttttcagg tgactgagcg
     3061 acttcaggat ccgccaccag cagatctttg tgtgactgcg tgaaggtggc cagatcggcg
     3121 cggtcggtaa tcttcatgta cgccaccatc ttctcgtcga acgggaagac ggaggtcgtc
     3181 gcgcctaatt ctgcccccat attgcagatg gtgcctttac ccgtggcgct gatggtttca
     3241 gcacccggac caaagtactc gacaatcttg ttggtgccgc cttttacagt aaggaggcca
     3301 cacagataaa gaattacgtc tttgggggaa gcccagccat tcagctttcc ggtcaatcgc
     3361 acgccgatca gtttgggatg cagcacttcc cagggaagcc cagccatgac ttcgccggca
     3421 tccgccccac caacaccgat cgccagtcct cccaagccgc cgccgttcgg ggtgtgtgag
     3481 tcggtaccaa tgatcaagca gccgggaaac gcgtagttct ccaaaacgac ctggtggatg
     3541 attccggcgc ctggcttcca aaacccaatg ccatactttt ttgcagctga ggcgaggaag
     3601 ttataaacct ccttgttctc atcgagcgcg cgaaggagat ccttctcgga tcccatttcg
     3661 gcacggatca aatggtcaca atggatcgta ctgggcaccg ccgccttttt cttgttggct
     3721 tgcatgaact gcagcacggc catctgggcg gtggcgtcct gcatggccac acgatctggc
     3781 cggagggcca gcatcgcctt gccacgttcc caattctggc tatcaaaatt gtccgcgtgt
     3841 gagacaagaa tcttttcagc aagcgtcaaa ccacgaccga acttctttct agccttggca
     3901 aaaacctccg gcatcttggc gtagagcgct ttggcgagat ccatggacat agtaggttct
     3961 ccgtctgtta gtacgatcta taggttgcgc gactcgttcg ctgcgaagga ggttgtctag
     4021 caggatcctt ttcgcaaaca acacgtcacg ttttgcgcta cttcagcggc ggcacttcgc
     4081 ggcgtttggg ggccgcgtaa ttcaccagat aatcgaacaa ccgaatcaag cggctatctt
     4141 gccgcttctg atccacccag tggccaatca agccaatcgt gcgagaaagg atgaagaatc
     4201 cgttcaaact atcaaccggg aacccgatat cgacgagtac agccgccatg gtcccgtcga
     4261 cgttcagaat gagattatcc tttttcaccg aagtgatctt ctccacctca agtgcaaaat
     4321 ccaaacaagg cgtcttgatc ttcaggctct taacataacc aaccagttcc ttgacccgct
     4381 tgtccggatt acgcaggctc ttgacacgat ggccgatacc aggaaccgga ccatgattct
     4441 tcttcatata ggctaagaat tcatcgaccg acatcttgtt atcgactgcg tacttaaaga
     4501 agcgaccggc atcggtcacg gctccgccaa aacggggacc aatcataatc atgccggctg
     4561 ccactgactg ggaaaggccg atgccggcac aggcggcaag aatcgtcgcg taggcgccgc
     4621 ttacgcaggg accatgatcc gccgagagca tcataatccg cttaatgatt tcggcttcct
     4681 gcttcgagat caagcgtttg tcccacaaga gtccgatgac gtgagggatt tcatagcctt
     4741 tgttgataag ttcggaggca ggatagccgt cgtagcacgg ttcatcgcca cggtcatcgc
     4801 tgattgtcgt gcgaatgagt ggcgcaacca tcacttcatc ggccttcatt gcttcttcaa
     4861 cggtctttgg taagcgaggc agaaccgccg gctcgactgg cgccttaact tggccggact
     4921 tcagcaggtc ctcatacgtt tccttaattg cgggaccaag cgcgccaaag gtagggggca
     4981 caagtgctcc cgctttcttg agggcatcgg acttcgaccg agcggaccct tcgcctttca
     5041 tgccttcctt ggcaccggca tggccgaatt tcattccttt gggcaaactc tcctggcaga
     5101 atccagatac cacggcgatc aacttcactc gccgcttctt ggccccatac cattcagctg
     5161 cgcgctcctc gaggtcgccg cccatttcgc ccacaatcac aacggctttc gtttgcggat
     5221 cgttttcaaa catctcaagg tagctgacgt aatccgtacc agggtaggca tcgccaccga
     5281 tcccgatcgc cgtcgtaatg ccgtcggcga actgcgaaca gatccaaatg atttcattcg
     5341 agagaccacc agacttggta atgacgccga acgatccttc tcgatacagc ttcgaaagga
     5401 cgaggttgtc aaatgcacca ccaatcacac caagacgaca ggcgccggct gaaataatac
     5461 cgatagagga tggaccattg aaaaccttac ccagcttgcg cgcatgggcg ccgagaatct
     5521 tcgcgtcctt ctccggcaca ccctcagtga tcattgagac caccttgata tgcgaatcat
     5581 caagcgcttc cattccgccc ttcatcgcac ggtctgcgcc aatgtagacc agactggtat
     5641 tgagggtcgg gtgattcttc gtcgcttcgg cgatagactt gtagacagga atcgcgatga
     5701 ggccgctgcc gtaaggtatc tcattggtct taccggcgtc tggcggatac acgaatgcct
     5761 cgacgttcag cggacgcttg atgagataac agaactcggc catgcggcgg gcggcgttga
     5821 caccggcttg accaccctgg atcaccacgc gggtgtcttt gtttgccaga atactcatct
     5881 cgggatctcc ccttatattc agttggtcat agacggcagg ctacttcgct tgaagtgcct
     5941 tgtcgacgat gtcggtaagc ggtgtgttgc ggtcgaagac attgatatca aagccctcgt
     6001 ctttcagcgc gcgcatggca tcaaggcctt ctttttcacg cggcccgcca cgacgcaccc
     6061 aaatcttcac atttttgagc ttgccttccg actttgcttt cctgaatccg ttgatgatgc
     6121 cgccgaatgt cttttttaca tcagtgaaat ttgcgatggc accacctacg atgatgtttc
     6181 ggataccagg aagagagcag actttctcgg tgagcacttc aacggcccaa tcgggtggat
     6241 ctcccgagta ttccgcatag tttgccaact tgccgcctcg agcgacgaca gcatcagagt
     6301 aatagacact ggcgcccccg ccggcaggca acatggcggt atcgccccca gggatttcga
     6361 tgaatttgac ggaccctttg atcttgctat cgacggccat cacctcaagc tcttgtttgg
     6421 aatatgctcg cccgaattcc gcggcaaagg caaagttcca atcagggtgc ctgaatttgg
     6481 catcaccgtc gagaagcgtg actgcatcca gggccaccag ttcatcatcg ccggctcgga
     6541 gaactaccgg attcacttca agatattgcg cgtcttcgct atcgaagcag gcaaacattt
     6601 tcccagcaaa ctccgccatt ttcttggcaa gcgcgccgga gaatccggct tctttgctga
     6661 gcttttcgag ggattcggga gagggagatt gcccgatgtc gaggcacaaa cgtttgacac
     6721 gttcccagtt agattcaacc tcaattccgc cacaattggc tacgaggatc tcactgcctt
     6781 cgcgtgtcga tttcacagca caatagtatt cttccttgtg cgggatcatc tcagagacaa
     6841 tgacctgggt cacagtgatg ctgccgactt ggcgaccaat catttccttg gtcgcagctt
     6901 ccgcggcctt aaaatctaaa tcgaccttga ccaatcccag tttaaaacgc gagccgagcg
     6961 cttcatgcgc tttggcgacc aacttcgact tcttcaacca ttcattggct tgccccaatt
     7021 tggtcagttc atcaactgaa gtgacgacca cataattagg aacgttgatt ccccacttct
     7081 tcatcagccc catcccggga ccttccagca ctttcgccat gattgacgac tccttggtgt
     7141 gtgatagggc agagaaacgg agcgacagag taccgaattc tgcggattag ctcaatcctc
     7201 caaagggcct acgcttcgag ctcagaagtc ctaccataat gggcactgaa gattcattac
     7261 cataaatgat tgataagctt aaattgtctc cttagttatc tgaaagaacc gagacggacc
     7321 ttccgatgct aaccaacttg cgggcgacga atgcgttggc accgaggaca ataaaaggaa
     7381 cttcgctcgc cgatggatcg cctgatcacc gctccacaat cgttcggaca aggctcgcct
     7441 gatttggcat agacaaggtg tcgacgctta tactgcccct ctgttccatc cggcgcaaaa
     7501 taatcgcgca cactggaacc acccagatca attgcctctc gcaagacgtc ccccatgatt
     7561 tcatgcagcc gtatcgctgc cttttggttc acttgattag caatttgatc tggatgaagt
     7621 cgggcacgaa acagcatttc attggcgtaa atattcccaa ttcctgcgat agcctgttga
     7681 tgcatcaaca ggggtttcag cctactacgc gtggataaaa gcaactgcac aaactgcttt
     7741 tgacttatca ctaaaggatc gggcccaaaa cgacgggatg tatatcgatc aagtccagct
     7801 tggtctaata acgagagacg tccgaatcga cgtggattcc aatatcggat ctcagattcc
     7861 gcgcctcctt caagcttcag gacaaagtgc gtatgttgcg ggtgtttagt cggcgtattc
     7921 cgaaacaaga gtaggccggt catccccaat tcggcagtca taaatttcac ttctgctgcg
     7981 cggataaaag cgaggatgac gcttttccct cttcgctcaa cccctgtaat cgttgctccg
     8041 cgaaaccatt ccaaggaaga caagccttcc cgcacaatgt cttcccgccc tacccagcaa
     8101 tcactcacca gggctccaac cactctctcg cgtagctggc gcgcagccac ttcagcttcc
     8161 ggcaattcag gcatgtatag gtttccgtat gtaggtaatg acacaaaaaa cagtaaatta
     8221 cgccatggcg acaaggagaa atgcaactcc caacagcagt tcctttaaac caattggcag
     8281 cgtaatagat gcgaaagggg ggaggcctgt agtgaaagga tgctgctaat aatgtcgggg
     8341 gaatcaatct aggagagaga aggtcgctct acagcacaac gtcggcggaa aacgtcctcg
     8401 atcaccttat cgaggcgagg tagcggcata ccaccaggac gccttgtgag ggcaagctta
     8461 agtgcatcgg aatacgtcat cccctccacg caacaaaggt aggcgatgac aacggacacc
     8521 gaacgcccca taccagcccg gcaacagacc atcaaccgat tgtcaggcag gtgcttttca
     8581 atccattgca ctgcgtcgta gagggctgac accgctggct caccaaactc cttcagggga
     8641 atcttggcat agatgatcct tgcggggaca gtcacatttt gttcttctgc caccatgagt
     8701 atcgctccga cctgcggcgg cgggttcttc gcatcatcag cattaccgac gagcagacgc
     8761 ttattgatca tgtgcatggt gaggttagta tagaattccg cgggaggggg tcaaggattc
     8821 ctgcgattcc atgcccaacg attgaagatt ccgtaacgca gtgcgtatac tcattcaacc
     8881 acttggctga tggggtaata acgatggctc ttattcagct tgaacaggta gaaaagcgcc
     8941 tgtccagcgt acaatgcgcg atctgcaaga caaatacatt cggcattgat cggcggactc
     9001 tgcaatcgga cggagaatgt cgtggggtgt gcctcaaatg ccgctacagc tttccggtat
     9061 ataccgacat ggaattttat ctgcgaacac aacctgatat tccttaccgg ctcaaggaaa
     9121 tttcttgtcc aaattgtcaa catcgtggcg tatctctcga cttccgcatc acgatgtctg
     9181 tccgtgaggc aatctatttc gtgacgtgca gcgcatgcca cactcagttt cccgagcgtt
     9241 cttccctgga agcttttgaa tagccctcgc ccccggcatt tcgtgtatac tgacttatta
     9301 tgaccgacac acaaaagccg aaaccgcccc tccctgtcgc tgatgtcgca ccaaagcctc
     9361 gcaaagaaat tcctctcgtc tcggtgccca acgatcggga catgattgcg gaggaaatgg
     9421 ctgcactctt tgatgaggat cgcgtgtctc atcagcacgg ctcctcagag cctgaagccg
     9481 attagcgctt cacgccggcc gaatgacttc cgccactccg gaggaaagaa ttcgaccgac
     9541 catggctggc cggcctttcc gttcttttcg gacgatctcg tcaacgcgaa cgattgcccc
     9601 acgatgccaa ttgtcgaacg tggcgttagt ggttgatctc tccttatctt gtcgaccaac
     9661 ttcagaccta cccgtctgat cgcacaacca cacccgtttt tcccccttca tctcccgcaa
     9721 ctcgcttacg acgcggtgat aatcttgctt tcgaggcacc aaggacttcc catctttacg
     9781 tatgatgaca taagaaaatt tgagagcgtc cttaataaat cccacttcgc gatcgatctg
     9841 actaatccag gaaggagttt gccactcgcg ctcttcatga caccaatcgt caggtttgag
     9901 caacgcggga cagagttggt catggaggca gggactgtag atcgagcatt gcaattcaga
     9961 cagcacatta tccctcacct gatgcagggc acgcgattca gccctcgatg ccggctcaat
    10021 gagcattagg cttccgtctt ttgccaagca gtctaccagg tgattgatca gtgttgtccg
    10081 tcgagcaata caatcggatt tttcggcaaa cagttccgaa agcacattct gaacaatgat
    10141 aaggtgatag gcatttttct ccgctcccat tcgaagaact ggaggaaagg agtgctcaag
    10201 atcgcacacg gcagtctgca atggttgaat agaaggtatg ttgatctgcc tggcgtattc
    10261 ctcccagagc cgggtcgaca gtttcatggc ctcgatcgaa cggtcaacca ccgtcatgcg
    10321 gagactgctt atgggtggca aagactgtga actgcaccag tcgagaaggg ctagtgagcc
    10381 ggtgccaggt ccgcccccga catccaacac tcgaaattct tcatttgtac gggatggaag
    10441 tagcggccgc aattcctcca aaagcatctg gatcttgccg acgttaacag gcacataata
    10501 atgaaggtag ctggcacgta gcgccccatc ttcaagatag gctgtcgcac cagctttggt
    10561 tcctctcgta aacatttcag aaagattttt cacgccactg actagcgatg ctgaaagtgc
    10621 tggattctgt agggagttat tgaccctagc caccgttgcg attgtgccta acattgccga
    10681 tgacagttgg tacatggagt cggcctcttg gttgaccctc taaagacggc tgcggtatct
    10741 tggataggag aggagtactt cacatgactg accccatttt gcaagcctac cgagaggtag
    10801 aaatggccat ggagcgcttt acgctggttc ttcatgacca tgtcgattat ctccgcaaga
    10861 ccgaggcgcc cggatctgat aagctgcatc gaatggccag tggcaccaaa gccatgcgtg
    10921 acagcgcatc catttatttg tcgtacgcca aatacgtggc tcacggtatg ccagaaagcc
    10981 ctgatctcgt ggaagaagat cttcagggtt aatcgcttgg aaccaaaaaa tgcagagggc
    11041 ccgttcgggt caaccgaacg ggccctccta tctgacgagg attacgccgt taatccttag
    11101 atgctgcgca tgaaatgccc catggcttcg tgatccgcag ccgaggcgcc catcacctga
    11161 gaaatcgcta cgttcgtcga cttctttcca gttagccctg attcaacttc gtcaacgatc
    11221 aattctaccg gcatggattg accaccataa acccgtggtc caccgataat cttcgcattt
    11281 gagaagccat agatggctgt cgccacttcc tttgccaacc atccgacgta gttaaattcc
    11341 ggcacgacaa taagcttggc ttttccacag agcttacgca actcttgagt ggggaaggga
    11401 cgaagggatc taaccttgat caggccaatc ttcataccct tttcagcgca aatgcggacg
    11461 gcttcgcgag attgagccgc tgcactacct gaagcaatga tgatggcctc agcatcctcg
    11521 acattttcag ccgtcagcag cccgcccatg tactgattaa tgtactttcg agagcgctcg
    11581 accgcagccc acacttcctg ttgccagaca gcgtgaatgt tgtaagccat aaaatttgac
    11641 ttctgaacgg gggcatcgcg agaaagccgg gccggaggat tctcggcatc caacactggc
    11701 acagctccac gccaaggctc acgcggcgga agcttcatac cacgatcttg catgcgcaca
    11761 tagcctctcg catgcgtcac aaagaacccg tcgcaacaca cgcccacagg aagagtcaca
    11821 tcattttttt cgctgatgac aaatcccgcc agcgtaaaat catacatgtc ttgctgattt
    11881 tcagcatgga acacaatcat gccgcaattg agcagatacg acacttcgat attgtcaggc
    11941 tgaatggcca atggtgcgtt cacgactcga caggtgaaca tcgccacaac gggtagccgg
    12001 tgaccgggcc atgacgcgat accttcaaga cctctcaaag tcccaggacc cgcagtggcg
    12061 gtgtagcatc gaacgcccgc tcgcgaacca cccgcgatag cggccatgac gccaacttct
    12121 tcctcgccac ggtaatattc cttcacatac ccctctccat acagaacacc gacgagctgc
    12181 atggtttcac tttgcggggt aatcggatag gcgatagcga ggtcaacgtt ggagcgacgg
    12241 atggcttcct ttgccgcctc gctgccagta ataaactctt tggtccgtgg agcttccaga
    12301 aacatatatt ccggcgtcac aaccttctgt tgctttgccg ccgcatgagg gtctttcttg
    12361 gtctctactt tcgcaggcga aacgctcgtg gctggctcgc tctggggacc ggtcttttgt
    12421 gcttcgctca tcatcacacc tctcgactta ttgccttagc ctaaacccct agccttctcg
    12481 cttcatctga tcaggagaat gaccaaacgc ggcggcacta cctgcaccgg cagcggaagg
    12541 cataaaagtc ataggatttg tatgcgtctt gccatcgtgg actttcgact gagtgccagt
    12601 gaagcggaca ttctccccat tgtcaggcaa tttaatgccc aattcttcac gaaccttttt
    12661 gaagatctgc gcagtctttt tcagctttag ggtgtcggag tctcgaatcg tcagcatggc
    12721 gtcttcatgg ccctgttctg cgcagatagc cattgtgcag caattcgtcc ctgcccggat
    12781 cattttgagg gtcagattgg cgtaatgaca atgcacacca ataaagatac aagcctcgat
    12841 cttgttaccc caaattgtaa gattgggatg attcggattg attacctctt caggatcgat
    12901 ctttggatac ttggggcgat aatcaggcat gggaataatc atgacgtccg ggatctgcgc
    12961 cgcgatctcc agaacggcct tggctttttc gactgcatgg ttgttccacg cccacaagac
    13021 gagaggtcct gggaagatcg ttgcattcgg gctcgtcagc attttccgag ccatttcttc
    13081 gatcactgtg tcttcattag gttcgaggag gccgtaatat agtccttgcc caggatcagg
    13141 caacgacact cccatctgtg ctgctgacgg tggatggaac cctgccggcc ctggcacgat
    13201 aatccgctct ctcgtatctt gcgtcgttcc cacgcccgtt cccctttcgt ccaattaccc
    13261 aactacaggg ctgaccaatg tcgccgtcgt acttttttcc ccataggtaa tattgtccgt
    13321 caatgccgcc accggcaact ggtcaatcat aatcatttta atggcattat ttttggccat
    13381 attgtcgcag acccacacac attgcgcgca gcccttgcac cgatctacgg ccacataagc
    13441 tgagccatac ttaaatccct tgtccttgcc catttcgtcg ctgtaaagaa tggtattggc
    13501 ttccgggcaa tactgtgtgc agagcttgca actttttgcg gcacagatct caacactaat
    13561 atcggctacg agatacatga gaactccttg tccgtgatcc ggattacgcc gtgacgcttt
    13621 cggtcgcttc cgaacgtttc acggatggtg cggcccatcc atggtcaaca gcataattcc
    13681 agcctgctcg aataacggcc acatttttct caatcaattc ttgcttcttc ttaaactttc
    13741 gctcgacgac gctatccaag gccgcggttc caccggatac gacaaaacct tttccgagga
    13801 atcgatcctt aacggactgc tccagtgcct cgactgtagt gagaccagta atagcgccga
    13861 tacaacccat caacgccata ttcgtagcaa ggtccatccc cgcaacctca agcgaaagct
    13921 tggtagcagg gaagtagtag agcttcgcat gccgctcttc gagttcgcga gcctgatcgc
    13981 ggtgcaattt catcgggccg tcattattga tcaaggcgat accattctct tttaagccga
    14041 aatagaatgg catcgtgtac gacttaccgt gcgtaataac ctgggggtga aagatgataa
    14101 tgatgtgagg gaaagtgatt tcgccaattt cataaatagg ctcatctgaa accctgacat
    14161 agctctcaac cggcgccatg cgcttttctg acccataaaa cggtacgatt gtactctcgc
    14221 cgccggcatg aatgaccgcc gtgctcagaa tgtgcgagcc ggtcacaacg ccctggccac
    14281 ccacacctgc catacgaata ttgaaccgtg tcgccatgct gaatcctcct taaacgttgc
    14341 tcaggccttc ggcagaccag gagcgggagc cgccggcttc gggaggaact ccttgtagcc
    14401 atagcgctcc gtgagaaatt gctttgcttc gtcggtaatg tattcggaga actgatagcg
    14461 atcgttttca acgttcttgg cgtcttccat gaccttgtcg gttggaatgg cgtattcgat
    14521 gttgcaggag gtataggctt ggatatacgt cgacccgacc tctctggcga tcagcacagc
    14581 cttcttaatc acgctctcaa cgcggcgcgg attgttcggg acaaccgtgg caacgtacgc
    14641 gcagccggcg accttcgcca tctgcaacat gtccattttt tcaaactttt tccccaacgg
    14701 agccatcttc agcacggcgc cacgattcgt cattccgctt tcctgcccac cggtatttcc
    14761 atagacttcg ttgtctaaca taatcgtggt aaagcgttcc ttccggaacc aggagtgaag
    14821 aacttgctgg aatccgatat cggccgtacc cccgtcgccc gccatcacca ctacgtcctt
    14881 cggcttatca ccaaaacgaa ggcgcaaccc tcgagacaag ccactcgcca caccgttttg
    14941 gtcaccatag ttaccgtaca caaaggggat ggcagcttgc gagattgcca aacgtccaca
    15001 gcccgctgtt ccaacagtga tagtatcttc gggattcggg aatgcgatca tggcaaggcg
    15061 gataaagagg gtcatggcac atccagcgca catggggtgc tcttccagca cttccttgaa
    15121 ggaccccatc tgcgaaacag agaccttttt cccaaacggg ccgtgctcaa ccatgtctcg
    15181 atattctttc ggcatgaact tttcaaagcc gctcgagaac ttgacgtaat caagactcat
    15241 aaggattccc tcccttttat gctctggtga ctttcctccg tcattccaaa atggccggag
    15301 aaagccaagc gcaaattata tcgacgttgt tcgaactaag tactggactg tgttcctgaa
    15361 acggaaagga atgtcgactt acgacctaac tgtctgatgc cgccgagtgt aacaaagcgc
    15421 aaaaaaatct gtcaatcaag tcggcgctag aaattaggaa gaaccctgtc attccacggt
    15481 aacggcaagt ctatagagtc cacaaaagca ttgcaacttt gcagaaggcc cacactgaac
    15541 gaaagaaggc ctagtggatg ccgccttgct aggcccttgg ccctaaatcc gcttgaactc
    15601 aaagattttg agcaaagaac aaacatgaaa taaggttcaa ttcagttgca gttcatctga
    15661 gaagcgcgta aaaattatcg cttaacacga taggcctcct aacatctatg gcgatacgag
    15721 ttctggtacc aggggaagaa cagggtgatc aacgtgtcgg aggcgtgcat aagcgcccgc
    15781 cgatcccaga cggctccctt aaggccgagt gcccaaaatt catgagccac ggtccttgcg
    15841 gaggggtgcg caaaggtggg ttttgcgaag tctatccgga catgacctgt ccgtgggtca
    15901 cactgtacaa caagctcaat gagctgggtc agttggaatg gatgaaacag gtatgacgat
    15961 tgccggtaag gggtgatggt gtgagagaac agaacaagaa gaaattgaag acaggaaact
    16021 ccacaaccaa gctcggctat aacgagcggc acgcacgcag cgggatctcg gcccctttgc
    16081 cagacattga aacctttcca aaccagtaca agggctatga aattacgatt gtcattcctg
    16141 aatatacggc gatctgcccc aagaccaatc tcccggactt tggaacgatt actcttcact
    16201 acaaaccgaa taaacagtgc cttgagctca aagcactcaa aatgtatgtc cacgcctata
    16261 gaaacatcgg tattttctac gaaaatgccg tcaaccgcat cctccaagac gtggttcggg
    16321 cctgccgacc gacttgggcc aaggtaacgg gtgagtttgc cgctcgcgga gggttgcgca
    16381 gcgtcatcga ggcaaaatat cccgaatagc ccgccaacta tccactgatc tttcgacatt
    16441 ccgccccgct gtacctgctc ctcctggccg attccgagca gctcctcagc acatcgcatc
    16501 acgcccccct tcttcagcaa ccgcgctgat cgcccttggg cctaaagtct cgcttcgaat
    16561 ccaccgacat cggacagtca aggtgtagga cgggtgcgag gtcacgcgac cacgctcggg
    16621 catgaaaggg tgccgttttg attacgcgct ccgcaaaggc tgtcattgtc cgactgacga
    16681 tgacggttct ggtcttcatt gtaggaatgt acgccgtgtc cgttcccact gcagacgcca
    16741 ttccatcatt tgcccgcaag tatgcactcg actgcaccgc gtgccataca gcccctccac
    16801 gactcaatac atttggcgaa cgcttcctcg aaaatggcta tcagctcccg ggcaccacag
    16861 acggcggcct aacgggaaag aaaaatctgg gggatgtgag cctggacgat gttgcgaact
    16921 acaccggcgt ccgcctgcgc ggtaatgtgc tccgcaccca tcacttcaaa cagcaaaatc
    16981 catccggtgc agaggcaggc acggtccaaa acaagagcga cctggccttc cccgaagtgt
    17041 tcagcctctt taccgccggc accttagcga aaaacatcgg gttttttgcc gaactcgaat
    17101 ccaacctcga agcatctcac accggaattg agcgggcctt cctcaccttc aacaacatcg
    17161 gaggtgaaaa cgtcgccaat atccgcgtcg gaaagatcga cccatccgca gccttttcct
    17221 tttcaacact gcgccaacaa ctggacttca ttgcggaaag cacgcacccc tcgacaaatg
    17281 ccgttcaacg cgcgggattg tttccgctgg caacggcagc caaattttac ggactgcgga
    17341 acagatcggg gggcacgatt tctccctatg ccccttcgct ctataacgca gtggtagaga
    17401 ccggcgcaga ggtacggggg cggcccttcg gagattggtt catgtaccaa ctcggcgtgc
    17461 tgaacggagc caatgaaggg ttcggcgatt ccaataaagg caaagacttc tatggagcca
    17521 tccggttcga ttacgcacgc tcagcaaatt tttcagccag cctgtcagga tttgcctatc
    17581 tcggcaatag caatgcgacc gtcttcgatg ggacacgaac gacggatgtg aactggagtc
    17641 gctacggcgt cgcggcacac ctgcgataca aaatgttcga tctgcacgga atgtatacgc
    17701 tcgatcgaat cacgcatgca ccagcgacgg ctctcagcaa ttttgacacg gacgccagcg
    17761 ggctcacggt cgcgctggat acgcacgtgg cgaacaatac gctgttttcg ttgcgatacg
    17821 acactatgga tgcgggggga gacctcagcc aacgaagctc tcaaagcttc ctcggcgccc
    17881 aggtgaagca ttacgtgcgg tcgaatatcg cactgtatgc gcgattggac cacaatctgc
    17941 gccacgcgga agacgggcaa gcggcagcca ggaatctgcg ggaagccttc tttgccggaa
    18001 ttgacgtggc ctactagatc ggcaggagac gaacattcgc catgggcatc agcatgtata
    18061 aatacctcgt catcctcgcg gtcagcgggc tctgcgcgac gagcgcctat gccgttcggc
    18121 ctgaagacaa tcgggacacc cttgcggaag gccgccggat ctatgagcga gcgtgtgcgt
    18181 ggtgccatgg gaacggaggc ttgggagatg gaccatccgg ttggtccatc ggccgttact
    18241 ccgcccctcg tccacgcgac ttcacaaaag aaagctacaa actacgcagc acaccgtccg
    18301 gcgaattgcc cacagatcag gacctgttca ggactctcac gcaaggaatt ccaggggtca
    18361 tgccatccta tcgctccctt aacgagtcag aacgatggca ggtcattgcc tatctcaaat
    18421 cattcaaccc tgccttcgag caggaaacgc ccgtatccat tcctctacct cctcccccgg
    18481 tctcacgacc cgagggcgcg gtacagcatg ggcgagtgct ctacgcaaag tacgactgtc
    18541 gggtctgcca tggagcaaac ggagaaggaa atggaccgga atcgcaggcc ggacgccttc
    18601 gtgacaccaa caatctgccc atacgcgcca cgaatctcac tgatccggct tcattcaaga
    18661 acggcagcac ggcacaagac ctctatcgat ccatcatgac tggcctcgac ggcacgccaa
    18721 tgccctcgta tgcgaacgag tttgccggac aggaagaggc ggtttgggat ttggtgtggt
    18781 acctccaatc cctttcaggc cgtccggaga aatgagtcac gccgatgatc atcacgccat
    18841 caagcgcaac gccttacgcg caccgcacag ccggcgaaga aaggaacggc cactcatggt
    18901 gatgaggctc atgctggtag tcctggggtt gaccctggga gttccctctt tgctgcaggc
    18961 cgaaagcccg accggcaagt tggccggcag gattacctat agcgggtcgg ccctgcctga
    19021 ccagacggcc gagatcactc gcgattctgc attttgcgga acaagcacca cgatcaaaga
    19081 cgtcgtgatc catccaaaga cgcgcggcct tgaaggcgtc gtcgtgagca tcgagagcgg
    19141 caaccttccc tcaagcgatg cccctcttgc tccgctcgtc ctgacgaata cccactgcgc
    19201 attcctgcca cgcacggtca taggacaggt aggccaacaa ctggagatcc gcagcgacga
    19261 cccggtcatg cacaacacgc acatctccct gggatcacgc acctttgtga atgtggcgat
    19321 gatcccggcc agccgaccgg tacgaaagcc tctcaagcaa cccggcatct atacggtcaa
    19381 atgcgacgca cacaaattca tgcatgcgga tgtgatcgcc tttgcgcatc catttttcag
    19441 ccagacagat gaaagtggtt catttcagat cacaaacctc ccaccgggtg agcatgtggt
    19501 gaatatctgg catcacacgc tgggaagcta tcaacaaact gtgcgtgttt ccgctgaggc
    19561 agagacgacc atcgcctttg acttcgccgc tcatgctgaa tccaacaaga aataagatgc
    19621 cctggtttca tcacctctgg atgatgtggc aagggagtct ccggctccgg ctgagtgtgg
    19681 ggatggcggt cctgatcacc cttggcatga cggtgttcgc caccatccga ctggtagaaa
    19741 tccgtcactc cctggtagac gccgcgcaag cccgcgcact ggcggtcagc cgcaccttcg
    19801 ccatgatggg agcggccgcc gttctggaca atttgtttcg cattcaagag gctctcaccc
    19861 gatatgccaa cgaggcggac attgtcggca tcgtcatcat cgatcccgac aatatggttg
    19921 tcgccgcgac aaagcctcac gatatcggcc gggaactgac cgatcacacc ctctctctcg
    19981 ctcaaacctc cgggtcggag acaatggccc atcgcctgag tgaggacgga accccgacgc
    20041 tgatcgtcgt caccccgctc aagaatgaag acgacatcgc cgcgtgggtt cgcatcgaat
    20101 tttctcttgc ggcaatgcaa ggcgagttgg tccgcgaagt tcgtcgtctg ttagtgcttt
    20161 ccgttctgct gatgagcgtc acgattgtgg tcggacaaat cggcatcagg cgaatgtctg
    20221 ccctctttcg agacacggcc gcgaaactgc aggatacgct ccatacgttg caccgcccct
    20281 tgaaagaaga cgcaggaaac gcaacgtcgc ttaacggcga ctcgcctggc cccgcgtcct
    20341 atacaggcga actggagcag atggtcgatc tggtggaaac gaccaccgcc ctgcttagga
    20401 cccaagcgca acggctacag tccttcactg actcgttgga agacgccgtg cggcagcgca
    20461 ccaccgaatt acatcaagcg aaggacgcgg cagaaacggc caatcaagcc aagtcacaat
    20521 tcctggccaa catgagccat gagatccgga cgccgatgaa cggcgtctta ggcatgacgg
    20581 agttgctgct cacgaccgcc ctcacgccga agcaacgctc cctcgcgaaa acgcttcacc
    20641 agtccgggac cggcctcctg gacatcatca acgaaatcct cgatttctcc aaaattgagg
    20701 ccggcaaatt gaaactggaa tgcattgaat tcggcttgcg gcagacggtt gaggaggcga
    20761 ttgatctagt ggcagaaaca gcccaccgga aacatctcga actgacctgc tttatccccg
    20821 aggagattcc ggatgccgtc gtgggcgatc cggtccggct ccgccagatt ctgctcaacc
    20881 tcgtcggcaa tgccattaaa tttactcagg ccggcgacgt atcggtcacg ctcagcctgg
    20941 tttctcagga agcctcccgc gtgaacctca aatgctgcat ccgggatacg ggggtaggga
    21001 tttccgacga ggccaaggcg cgactctttc aagcattctc acaagcggac gggtccacga
    21061 cgcggcgctt cggaggcaca ggtcttgggc tggcgatcgt caaacaactc gcccacttga
    21121 tgggaggcga cgtcggcgtc gatagtattc ccggccaagg atccacattc tggtttaccg
    21181 tctcgatgga atacgcctca caagctgtct ccgcccatga aggcctggcg tcaccgctgg
    21241 cgggccactc gatcctggtc gtcgacgaca attccactaa ccggcacatc cttgaggcac
    21301 acttatccaa ctggggagcg acggtgcaat ccgccgcctc gagcgaagat gggctgcgcc
    21361 tgctcaccga ggccaaaggc gggaatcaga agatccagat ggcggtgctg gacattcgcc
    21421 tccctggcat gaacggcatc gaccttgccc ggaaaatacg ggaagacgta gacctgcgcg
    21481 acatggcgat cctcgccctg agctcagagg atcgcgagac ggacgaatct caccaccatc
    21541 ccaagctgtt caatgcgtgg ttgagaaaac ctgttcatca gtcattgctg aaagattgtc
    21601 tcacgcgcct ttgccttgcg ccgtccgctg aagacgcttc tgcaaccatt cagcctcccg
    21661 aacagacgag aacattggcc gggcatatcc tcctggcaga agacaatcct gtgaaccgcg
    21721 aggtcgcgtg cacaatgctc gagatcctgg gatgcacgac cgccgtggcg gagcagggca
    21781 aagaggcggt cgagacggcg gcgcatgggg catttgattt gattctcatg gattgtcaca
    21841 tgccggaaat ggatggcctg acggccacgg ggctgatccg cgcccatgaa cagcaatcca
    21901 tgccggcccg acacacgatc attgtcgcgc tcaccgccaa tgcattggag ggggaccgtg
    21961 agcgctgcct tgccgcagga atggacgatt acctgaccaa gccctttacc ttggacgcgc
    22021 tgaaggagct gctccaacgc tggcttcccg aacatgtccc ttccgactcc acttcagact
    22081 cgcagccggc cgaagtatcg aacggggttc cacctcaaca ggccgtttca tctcatgctc
    22141 agcccttggc agcagagagc gagcatgtgg acgcgcatgc ctgggaagcc attcgagccc
    22201 gccagcgacc aggccagccg gactttttac ataaagccct ttcgctctat ctcccctatg
    22261 ccgacaagca gatcgctcgg ctagagaacg ccatcgccga ccaggatgct cgagccatcg
    22321 ccacgatcgc ccacacgatg aaatcctcct cagggcaact gggggcacaa cgccttgctc
    22381 tcctgtatac cgaacttgaa tccgcctcac gcgcaggacg gatcggggac ttccaggaac
    22441 tgtatgaccg cttgattcct gagcatcgcg cagtctctgc cttgatgcgn nnnnnnnnnn
    22501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cgagagcgaa caggtcatcg cccggcaaca
    22621 gtacgagtcg agcctgcgtg agagcgaaga gaaattccgg cgcctcttcg agtcttcgcg
    22681 cgatgcgatc atgattctgt acccgcccaa ctggaatttt agtgcctgca acccggccac
    22741 ggtccaactg ttcggggccc gggacgaggc gcacttcacg tccctcggcc caggagatgt
    22801 gtcacccgaa taccagccgg acggcgagtt gtccatggtc aaggcgcgca aggccattga
    22861 ggctgccatg cgggaggggt ttcatctctt cgaatggatg cacaaacggg ttggcggacc
    22921 aacctttttg gccactgtcc aattgactcg tatcacactc caaggtgtcg agggattgca
    22981 ggccaccgtt cgcgatatta ccgaacaacg ccgcgctgaa accgaactac gcgcctatgc
    23041 cacttttcaa cgagccatcc tggaccatgc cggctcggcg attatttcct gcagacctga
    23101 cggcatcatt caattgttca atccggcggc agaagcgctc ctgggttaca ccgccgacga
    23161 actgatcgga cgtcaacatc cagggatctt ccacgttcgc gaggaaatcg tggagcgggc
    23221 cagacaactc tccgaggaat tcggctgcac cattgcaccg gggttcgacg tctttattga
    23281 aaaatcccgg cgcaatcttc ccaatgaaca tgagtggacc tacatcagga aagacgggac
    23341 caggcgaacg gtgctgttga atatcacggc gctgcgtgac gccgacagca agatcacggg
    23401 gtacctggcc gtcgcctcag acattacgcc actcaaacgc accgcccagg aactcctgct
    23461 ggcgaaagaa gctgcggagg ccgccagccg cacgaagtca caattcctcg ccaacatgag
    23521 ccatgagatc cggacaccca tgaacggcgt cctcggcatg accgaactcc tgctgaccac
    23581 cgcactggac acccggcaac gccacctgac tcgcacgatc cagcaatcag gcgaagccct
    23641 tttggcgatc atcaacgaca tcctcgactt ctccaaaatc gaagcaggca aactgcagct
    23701 ggaacggctg gactttgatc tccaggacac cgtcgataac gccgtggaac tctttgccac
    23761 tccggcccaa cgcaaaggac tggaactcac gtgccacatg ccgagtgcga taccgcgcac
    23821 tcttcgtggc gatcccatcc ggctgcggca ggcgctgctg aacctgatca gtaatgcgct
    23881 gaagttcacc tcaacgggcg aaatcaatat acgtattgac gccgtggtcg agaccgacgc
    23941 ctctgtcacc ctgcggtttg ccgtgaagga ttcgggcgtc ggcatccccg ccgaggccca
    24001 tcagcgcatc ttcgaggcct tttcgcaggc cgatggcaca acgacccgcc gcttcggtgg
    24061 cacaggcctc ggactgacga tcgtgaaaga actggtatcc ctcatgcagg gacagatcgg
    24121 cgtggagagc caggtgggcc agggctcgac cttttggttt accgctgtct ttgagcgaca
    24181 gcctgcgact accggtgatc cgtatccgga acatgccctg ctccaacgac ggatcctggt
    24241 ggtggacgat accgccgcga accgcgagat cctggatgac cacctccgtt cctggggcgc
    24301 aatccctgcg ctcactgcat cggccgccga agcgctgacg caactccgcg cggccgccgc
    24361 gaggcaacag ccattcgagt tagcgattct ggatttccag atgccggaca tggacggcat
    24421 gcagctggcg caggcgattc gggctgatcc gcaattgtcc tcactccccc tcttgatgct
    24481 cacgtccgtg gggtatgacg ccggagctcc tggagtgccc gcggtcgatg gctgggtcac
    24541 caaacctgtt cggaaaacct tgctgcaaca ggccatcctg ggcctcttca ggacagggca
    24601 gcgtgcagca atacgccggc cggatccggt ggtatcggaa ccagtgacga ctgtctccca
    24661 ctcggcacgt cttctcctcg tcgaggacac accggtcaac cgggaggttg ctaccgggat
    24721 gttggacgtc ctgggatatt ccgtgcatgc ggtcgagaac gggcgtctcg cccttgaggc
    24781 cgtggcacga gaacagttcg atcttattct gatggattgc caaatgccgg aaatggacgg
    24841 cttcaccgcc accgccgcta ttcggcaaca ggagcgtcag tcaggtggag ggcatcgcat
    24901 cccagtcatc gccctgaccg cgaacgcgat ggacggcgat cgcacccgct gtctcgctgc
    24961 cggcatggac gattatctgg ccaagccctt taccgcggcg caactccgca caatcctgac
    25021 gcattggctg acaccgccgg tggctgacgc cacgactgct tccatgccca cgcagacggc
    25081 ccccccacgg gaatcgaccg acgaaagtcc gcaacctacc gccaaccccg agatcgacaa
    25141 gactgcctgg gaggcgattc gctcgctgca acgcccggga cgcccggata ttctcgcgaa
    25201 ggtcctcaca acctatttga acgactcccg gattctcgtg gaggaaatgc gtgctgccgt
    25261 agaggctcgg gaccccggca ccctcgccaa agccgcgcat cgactcaagt ccagcagcgc
    25321 acagctcggc gtgttggcca cagcggccca ctgcaaagag ctggaacacc tgggccgcct
    25381 cgcttctctc aatgatgccg cgcatctgct ggcccaactg gctgagacgc atcaagccgc
    25441 ctgcaccagc atcgccgccg aacttcgaag ccgctcctcc acatgaccgc ttagatcccg
    25501 cgccctttcc cgtcacgcac attcctaggc tgcccggcat tcacttccag ccatttcctc
    25561 agtggaatcg tcaagttacc tgcaccctcg accgataacg gagagataag tcctattcca
    25621 tggagtcgca catgcgctgc cttgccagaa cctacaactg gatgcttccc acaacgaccg
    25681 gctgcctctt agcggccacg atcctgggga ccccgctcac ctctttggcg gcctcgccga
    25741 tcgaagacgc tcccttggtg caaacggagg cctcgctgga tctccccgac ctaccctcct
    25801 caggcacacc ggacggtcaa gagcccgtcg ggaaggaaag tacgctcttc gaggagattc
    25861 cctcagtcta tggcgcggcc aagtatgacc agaaggtgaa tgaggcacca gcggccgtca
    25921 tcatcatcac ggccgaccaa atcaagaagt acggctacag aaactttgcg cagattctcg
    25981 acagcgtgcc gggcctgttt accagcaacg atcgaaacta tggctacctg ggaattcgcg
    26041 gcttcaaccg gccgggcgac tatacctctc gtgtcctgct gttgatcgac aatcaccggc
    26101 tcaacgatgc ggtctacgac cagggcgggg ccggcaccga aatgccgatc gacgtggatt
    26161 tgatcgatcg ggtcgaaatc atcaaaggcc ccggctcggc cttgtatggc accaacgcgt
    26221 ttttcggcgt ggtcaatgtg atcacgaagc ggggacgcga cctcaagggt gcggaggttt
    26281 ccggagaagc cagcagcttc aatacgtatc gaagccgcct cagttacggc aataagttct
    26341 caaacggcct cgagatgctt ctgtccggct ccttctcgga tagcgaaggg catcgccagt
    26401 tgtcctaccc ggcattttct tcaatcaata acgggattgc gaaagatgcg gaccgggaca
    26461 atctgtacta ttacaccgga aaactctcat acggagactt caccctcttg ggcggccacc
    26521 agtggcgcaa aaagaacgtg ccgacggcct ccttcggcac ggtattcaac gacaatcgac
    26581 agaacacgct cgacgaacgg acctatctcg atctcgtcta tcagcacgaa ttcgtcaacc
    26641 agctcaatgt gaaagcgcgg gtctactacg accgctatta ttatcgtgca caccttccgt
    26701 acgactatgc cgggaccggc acaccgccgt ttacactcaa caaagactcc acagaggccg
    26761 actggtgggg ggcggaactg acaatcacca agaggctgtt cgaccatcat aaattcacca
    26821 tcggctcaga gtatcgagac cagtttcgcc tgaatcaggc caacgccgat attgcgccgc
    26881 ccagcacata tctcgacgac aaacgtcgat cgagctttat tgcggcctat atgcaggatg
    26941 aatgggccat caccgaccat ctcgtcttga ccgccggtct ccgccacgat cagtacagta
    27001 cgtttggtgg taccaccaac ccgcgtgccg gcctcatttc gacgtgggat aagaccacgg
    27061 tgaaattgtt gtacggccag gcctttcggg cacccaatcc gtacgaacaa ttctacgtga
    27121 cgggatccac gttcaaggcg aatgccgaca tcaaacccga gcgcatcaaa acctatgaac
    27181 tagtggtcga gcagtacctg ggggatcacc tccgcgccct cggcagccta tatcaataca
    27241 acatttccgg tttgatcagt cagaccacgg atccgacaga cggaaagttg ttctatagca
    27301 acgtcgacaa catcaccgct cgcggcgtgg agttggcgat ggaagggaaa tggccctcag
    27361 gcctcgaagg gcggttgagt tatgccctcc agcgcaccga aaacgacgat ctcaaacaac
    27421 agttgacgaa ctcgcctcag cacctggcga aggccaacgt gattgtcccg gtgatagccg
    27481 acaagctctt cgccggattc gaagggcgat acaccagttc acgcctcacg ctcgccggaa
    27541 acagcgccag tcccttttac attctgaaca tgtcgctctt cagtcagcgg atcgtcaaag
    27601 ggtgggagct gtccggacat atcaataacc tcatgaatca gaaatatggg tatcccggct
    27661 cgggtgagca cgtgcaggat gtcatcgatc aggatggccg gacattctgg gtcaagctga
    27721 agtaccgctt ttaaggaacc ggcatcaccg atgcgtcgac ttatccacct catagcggca
    27781 ctagggatcg cgctcaccct gagcgcgctc tggctcattg cgagccccga ttcggcctgc
    27841 gctgaatccg gccgtatcgt ggtgttgagc gggcaggacc tcaaacccta tcaggacgta
    27901 ctcgcaggac tgcaacaatc gctgaccaaa caaggcatca atctggcggt ggaaatccat
    27961 gcggcccaag gcgctccctc gaccgcgaac gtgcttgaag acatcaagaa gaatggcgcc
    28021 cggttggtgg tgacgctggg aagtgccgcg acacaagccg ctgtgcgtga agtgggacag
    28081 gtccccatcc ttgccaccat gatcgtcacg gccgatgaca tcaagtctgc ctccaacgcc
    28141 accgccgtct tactcgactt tcccctggat ctccaaatgc agtggctgca tcggatcgtc
    28201 cccgccgcca ataccgtcgg cgtgctcttt aatcccaaag aaaaccagac caaggtgaac
    28261 catgctctcc gcatcgccaa agaaaacggc ctgagtcttg tgacacaagc ggtggacaca
    28321 cctcgcgccc tccccgacgc attggagaac ctctcgcgcc atgtcgatgc gctctggggc
    28381 atctcggatt cggtcgtgat gacccctcaa accgccgaac cgatcctgct gagcaccctt
    28441 cggaacaagg taccgctcgc aggattgtcc gcgtcatggg tcaaggcagg cgcactgtat
    28501 gcgctcgatc gggactacct cgacatcgga aaccaatgcg gagacatcgc cgggaaaatc
    28561 ctcgggggga ccagtgcctc gtcccttgcg ccgatatttc cgcgcaaagt gacctactcg
    28621 gtgaatctga agacagcagg cgccatgaac ctggaattgc cacaggatgt cgtgcgcggc
    28681 gcgacgcata tttttcagta actgcggcga ggatgacgat gataaaaaaa ggaatcggtc
    28741 tacgggacaa gctcaatctc ctgagcatcg ggctcgtcgt cctcacggca atcggcatcg
    28801 cgaccttcgt cgtgcatcgg gaaatcacca cccgctatca cgatctggtc aacaccggtc
    28861 tcaccaccgc cgcgatgatc gcgcagaaca gcgaatacgc cgtctatacg gaaaaccagg
    28921 atgccgtgcg gcgggcggtc ggaggacttc atgccttgcc ggatatcgcc tatgccgccg
    28981 tgctcggcaa agagcggctg ccgatcctgg aagaaacctg gggcgccaac atcgctatcc
    29041 ccacggcgct gagaacacgt ctacttgccg caggagacgc gctgttcgag gaggtggcca
    29101 tccagcaagg gcatccggcg tatatcgata tcctagtgcc cgtcgtgagt cggccggcag
    29161 cggctgccga tccgctcttt ctggagtcca atgcttcctc cccccctggc gcggtcatcg
    29221 gctacattca gctgggcctc agtcaggaac ggctgcgaaa gaatcttcag gaatttgttc
    29281 tcatcacggg acttgtcatc ggagtcgtgt tgctggttgg actctgcatg tcgttgatcc
    29341 tgaccagaag catcacgaaa cccattctgg cattagtgga agcggccgga cacatcgcgg
    29401 aagggcgcct ggacgtggat gtcgctgcag gcacccatga tgaggtggac cggctggcgg
    29461 acgccttcaa ccggatgacc ggccggttgc gagtctcaca gagtcaggtg caggaatacc
    29521 agcagagcct ggaagacaaa gtgactcagc gaacgaagca attggaactc gcgtcgcaag
    29581 aagcccagcg actcgccgaa gaggcgcagg cggccagccg cgccaaatca cagtttttag
    29641 cgaatatgag ccatgagatc cggacgccga tgaacggcgt tcttggcatg accgagctct
    29701 tgctgtcgac cacgctcgac acccgtcagc gccacctgac caagaccatt cagcaatccg
    29761 gcgaagcgct tctggcgatc atcaacgaca ttctcgactt ctccaaaatc gaagcaggca
    29821 agcttcagct cgaacaattg gatttcgatg tacaagacac ggtggaaaac gccgtggaac
    29881 tgtttgcggg gcctgcccaa cggaaacaga tcgaactgac ctgtcagctg atggggccct
    29941 tcacacacgc gctccgcggc gaccccatcc gccttcgcca ggcattactg aatctcatca
    30001 gtaacgcctt gaagttcacg gccaaaggcg agatcaatgt gcgcgtctat gcggtgacgg
    30061 aaacgaacac gaccgtcacc cttcgattcg aggtaaaaga ttccggtgtc ggcattccag
    30121 ccgaggcgca tcagcgcatt ttcgaagcct tttcccaggc cgacggcaca accaccagac
    30181 gcttcggtgg cacgggactc ggcctcacga tcgtcaaaga actcgtggcc ctcatgcaag
    30241 ggcagatcgg cgtggagagt caagtgggcc agggttccac attctggttt acggcggtct
    30301 tcgagcgaca accggcagcg gctcccggac aggaacaggc gcccgaacaa gccttatcca
    30361 agaaacgcat cctggttgtt gacgacacgc ccgctaatcg cgagatcctg tatgaacatc
    30421 tccgcacgtg gggcgcgctt ccaaccttgg ccgcttcggc acaagaagca ttggtactgc
    30481 tcggaaccgg ctccaactcg ccacagccgt tcgacctggc cattcttgat ctgcacatgc
    30541 cggacatgga cggcctggag ttggccaagg cgattcgcat ggatccacgc ctcgccggct
    30601 tgccgctctt gatgctgaca tcggtggggt atgacgccaa gacccccggt acgccggacc
    30661 tggatgcctg ggtgactaag cctgtccgga acaccctgct tcgccaagcc cttctcggcc
    30721 tgcttcacac caggcatcgt gcaccagttc agccgcccgc gccgctcccc tccgcgtccg
    30781 gcccgattcc gttccaggcc gcccatctcc tcctggtcga agacacaccc gtcaatcgag
    30841 aggtcgccct cggcatgttg gacatgctgg gccacttcgc ccaggccgtc gaaaacggac
    30901 gcctcgcggt cgaagcggtc gcgcgggaac ggttcgatct cgttctcatg gattgtcaga
    30961 tgcccgaaat ggacggcttt actgccaccg ccacgatacg acaacaggaa cgggacgccg
    31021 cagaccaccg ccatgtgccc atcattgccc tcaccgccaa tgccatggaa ggtgatcgcg
    31081 cgcggtgcct ggctgccggg atggatgatt atctggcgaa acccttcacc atggcacagc
    31141 tgagtgagat gttgacgcga tggctgaccc tacaggcgac tgctgcgccc atggccccag
    31201 gcccttcccc atcggcccgc caggacgact ccccgtctga tcagaccccg ccgtccgctc
    31261 ctgtggagat tgacaggaca gcctgggacg cgatccttgc gcttcaacga ccgggccgtc
    31321 ccgatatcct agcaaaagtg ttgacggcct atctgaacga ttcccgcacc ctcgtcgggg
    31381 agattcgcac tgccgttcaa actcaagacg ccgtcgccct tgccaaggca gcgcaccgat
    31441 taaagtccag tagcgcgcaa ctgggcgcct tggccacggc cgcccattgc aaagaactgg
    31501 aaaacttggg acgcctcgcg cgtctggatg atgctcctag tctgctggcc caactcaccg
    31561 acgcccacca ggccgcctgc gccgcaatga ccagtgagct cttgcaacgc ccagccgcct
    31621 gattcgacac tcacggatcc gttcgcgact tgcgaaccaa gccggtccgc gctagtctgg
    31681 gcccacccat ggctacctat gcaatcggcg acgttcaagg ctgcttcagc tccgtcacac
    31741 ggctcacgaa gaccattcaa ttcgatccca agcaggaccg gctctggttt gtcggcgatc
    31801 tcgtcaaccg tggccccgac tcgctcaagg ttctgcgtta catccgtgct ctcggccagg
    31861 cggccatcac ggtgcttggc aatcacgata tcttcctgct cgccgttgcc gccgggctga
    31921 cgtctcctcg tcccagtgac accctatccg cgattttgga ggcgccggac tgtcgtgaac
    31981 tgatgctgtg gttacgccag caaccgctgc tgtatcggga gggcgatttc ctgctggttc
    32041 atgcgggact gctgcctgcc tggactgtcg aacaggcaga gacattggcc ggcgaggcgc
    32101 acaacgcgat acggggagac cagtttctga cgaccttgcg cgcgctccat cccagcggtt
    32161 cgttgcaatg gcgccctgac ctcacaggcc cggttcgggt agccaccatc atcaaagtgt
    32221 tgactcgttt gagagcctgc tcggatgacg gcatcatgga atcttctttc tctggaccac
    32281 ccgatcagac cccggatggc ttttacccct ggttcgatgt gcgcggccga cgtagcacag
    32341 cggcaaccat cgtcttcggg cattgggccg ccatgggctt gcggctcaca ccaaacatgt
    32401 tggcactgga cagcggctgc gtgtacggca gacagctcac ggcagtgcgt ctggaagacc
    32461 ggaaagtgtt tcaagtcggg tgcgaggaga ctcgtggcgg aaagtgatca gccggttcta
    32521 gccctgtggc cgcggtatac cgaccgaccc cttccagctt accgcttcat tcccggacga
    32581 acgccacatc ctcgccgtca cccgcaaggc cattcattcg aacagcccga tcctcttctg
    32641 cgcccgttcg agccagaaca ttggaaaacg tccgaagact atctgtacgg catcgatctt
    32701 tataactgga cctactggtg ggaagcgcac gaagtcttcg aagcgttctg gcatgcttac
    32761 gggaggcata caccagccgg gaatttcttt caggccctca ttcaatgcgc cgcggccaat
    32821 ctcaaacggg aactggggca cgaacaggcg acacgaaacc ttgtttcacg ggcgctcgct
    32881 cgcctacaaa aatgtcccgt tcactatatg ggcttggata cggaaatcat cgccgaacag
    32941 ttcacacagt ggctgaatca gagggagcca cacgggcacg ttctgtttgc gttatcgaaa
    33001 gcaggcggga aaacacggaa cagttgaacg ccagtgggag gctactcagt ggtagctttc
    33061 cgagtcggac gggaacacac ctttctccac atcctccttg tactgacgga gagcctggag
    33121 cgcctcgacc ttgaggtggc cgtacggctt tacaaacttc ggcacaaact cgtcaaacaa
    33181 tcccaacaga tcgtagagca ccagcacttg cccatcgcag tgcggccctg cgccgatccc
    33241 gatggtggga atcgtgagcg cctcactcac tgtctttgcc aatcccgctg gaatcgcttc
    33301 gaggacaatg gcggccgcgc ccgcctcttc cagcgcgcgc gcatcctcca gcagctgttg
    33361 tgcgcgatct ttgccctttc cctgtaccct gtagccgcca tattgctgga ctgactgagg
    33421 cgtcatgccg agatgcccga tcaccggaat gccgacgttc gccatcgccc tcacacgatc
    33481 cgtcattgcc gcaccacctt ccagtttgac cgcatgggct ccgacttgaa ggagtcgccc
    33541 ggcatttcga accgcttctt cctggttgac ctggtaggag agaaacggca tgtcgccgat
    33601 gaccagtgag cgctggaccg ctcccgcgac cagcttggta tggtagagca tctcgtccat
    33661 ggtcacggaa agcgtgttcg ccttgccttg cacgaccacg cccagcgaat cgccgactag
    33721 aatgacgtcg atccctgcct gttcgacgat acgggcgaag agggcatcgt aggccgtcac
    33781 caccgcaatc ttgcgtccgt cgcgcttgca cttctgcaag tccgggacag tcatcacccg
    33841 agctcagctt tcgtcagaat ttccggcagt cgttcggtgg atttgatggc cataatgtgg
    33901 acgccatcgc agtagggacg gacggccttg atcgttcgca cggcaatctc cacgcccgcg
    33961 tcgagcgcgc gcttatcacc ggcggcacgc atttcgtcga tcatgtcctg cggaacacag
    34021 acgccgggga tattggcgtt catgaattcc gccatcttgg cattgcgcag cagcagaatt
    34081 cccgcgagca ccttcacctt gaacggacgc actgcgtcca tgaacttctt gaattgttcc
    34141 ggctgataaa tcgcctgggt ctggaaaaac tgcgcgccgg cgcgcacctt gacttcaaat
    34201 ttgaccagca tcggccccag cggatctgct tccggcgtga ccgccccgcc gatggtaaag
    34261 tcggtagaac catcgagctt gttgcccgca tagtccttgc cattattcaa tccggtcacc
    34321 aactgcatga cttgcacgga atccaggtca tacaccggct tggcgtcctt atggtccccc
    34381 acggtggggt agtccccggt caagcagagg atgttgcgga tgcccaacat atgggcgccc
    34441 atgaggtctg actgcatcgc gatgcgattg cggtcccggc aggtcaactg catcaccggg
    34501 tcatggccca gttcatacag aatccggcac actggcagtg acccggcccg cacgatcgcc
    34561 gcagtgttgt ccgtgacatt gaccccgtgc acgcggccga ccagcgactt tgcgctctcg
    34621 accacatggg tgatgttggt acccttcggc gggttatatt ccaccgtgac ggcaaattgc
    34681 ccctgcgcca gcacatcctt gagtcgccgc ggctcccggc tcatcgagat cttccctgca
    34741 tggtttgtgg tctccctatc ctccgagtca tcttccccta cctcacttga aagaggcggg
    34801 tcgggacgtc ccgattacgc gcatcgaccg agcacattct gatcgtacgc ggtctgcgag
    34861 caacggcgcg gcccgatctg cctatttcat gcctgccatt ctcttagcag attcaacggt
    34921 attgtcgagc aacatcgcaa tcgtcatcgg cccgacgccg cctggtaccg gactaatcca
    34981 cccggccttc tggctcaccg gctcataatc gacatctccg cagagcttgc catccggccc
    35041 cttgttggta cccacgtcga tgacgaccgc cccctctcgt accatatccg ccgtcacaaa
    35101 cttggccttc ccgatcgcca ccagcagcag atccgcctcg cggcagaccg cggcaagatc
    35161 cttcgtcttc gaatggcaaa tcgtcacggt ggcattgcgt tgcagcagca tcaacgccaa
    35221 cggcttgccc acgatattgc ttcgccccac aaccacggcg cgcttgcctt cgatggaaac
    35281 cccggccgat tcgatcatct tgatgacccc cttcggcgta caggcttcaa acaccgggtt
    35341 gccttcgacc aaccggccga agttgtatgg atggaatccg tcagcatcct tgttcggcga
    35401 gaccgcttcc agcaccacgc ggctctcgat atgcttaggc agcggcagct ggaccaggat
    35461 gccgtgaatc ttcggatcgg cgttcttctt ttcgatcaac gctaaaagtt ccgcctgggt
    35521 cgtactggcg ggcagcttgt ggtcgtccac ataaatcccg gccagttcac aggctttctg
    35581 tttattcttg acgtagacgt gcgacgcagg atcgtcaccc acgaggatcg tggccaggcc
    35641 cggcttggtg cccgttttag ccagaaccgc cgcagagtca accgccaacc gttcacgcac
    35701 ctgttgcgcc aatgctttcc cgtcgataat tcgtgccgcc acaatatcct ccctggcctg
    35761 agtagaagcg aaaagtgagg gcaccatagc aggcaatttt tcaacaagtc aacgctggag
    35821 gcggcgggac gcatctgatg aaagccctcg attccacagg gacctcgcaa ggtcgtgccg
    35881 ttgtctgtcc gtccggcctt ctccgctcgc tcatacgcga tgttgctgct tccttgacag
    35941 gttccgagac ccttggctac gatgcatcag gacaatctgt gcgaccatct tcgttgatga
    36001 ttccatgacc atcttttcct tctcttcgac acggccgaga ggccgtcgca accttggact
    36061 gactcttgcg ggcgccatgg gaatggtgct ggcctcctca tgggcctggg ccctgcaagt
    36121 cagcggattg gaatctccac acagctttct ggctgatccc ccctcgaggt cctatttcat
    36181 ttccaacatc aatggggaca gcaacgcccg ggataacaac gggttcatta ccaagctgag
    36241 tgacgatggt cgcattaccg ccttcaagtt catcgaagga ggtcgcggag aggtcacgct
    36301 ccatgccccc aaaggcatgg ccgtcgtcga tcaggtactg tatgtcacgg atgtcgacac
    36361 cctgcgcgcg tttgacaaga acacgggcaa acccgtcgcg acggtaatgc tcccccggcc
    36421 cgccgacaac aagaccaccc agtcgctcgt ggatgtggct gccgatggaa agggccattt
    36481 gtatctcgcc gaccagggcg gcaatgcaat ttatcgggtc gatctgacct ccccattgac
    36541 gctgtctctt tatgtgtccg gagcccattt ggccggtcct tcaggagtgg ccgtgaatcc
    36601 caaaacgggg catatcgtcg tggtgagtta tgattccggc aagattttcg acattactcc
    36661 ggaaggagcc ctgaccgaat tggcctccaa cggatttttt acgggacgct ttcaaaacct
    36721 tagcggagtc gatttcgatc gctggggaag catgtacgta tcagatgcga ccaaaggcaa
    36781 gatctggcgc atgttgccga acaacaaatt tcaagtcatc gcggaatatc tgcccgggcc
    36841 gtcagacctc ggcatcgacc gcgtgaatca cttgattctc gtgccgtacc aggactcgaa
    36901 tgcggccgag gtcaacggcc tcgaatctcc cacggtgtca tctggtgatc gtgccaaacg
    36961 caccttggcg gattacgggt ttggtgaacc tctgaaagct gacaaggaag gatcggcccg
    37021 caaatgagcc agtgtgtcta ctgtaagcag cgcaaaggga aacgttcctg tccggccctc
    37081 acggggctga tttgcagcca atgttgcggg gagcatcgcc tggcccgtat ctcctgccct
    37141 cctgattgcg agtacctcga cgcggggagc gactatcaac agaaacggct cggcgaacag
    37201 ttcgccccgg tccgtcggga actctatcgt cagctcagcg tcgccggcgg cgaaaaggcg
    37261 gcagccttgt tcaacctgat cgaagtcgtg acattcggct atttccatga ccggcgggat
    37321 gggcaggatg ccgaagtctt cgcagccatt caagcgttgc gccgcaccct cagtcctctc
    37381 catgtgcccg ccgcgccaat gccggtcttt gcggaacgtc tcaagaaaga atatgacacg
    37441 ttcgtcaagc agcaaccgca gcaaatcgcc gacgccggct cagccccgga aattctcgat
    37501 cgcgcgttgg cgctcctcac ggaattttcc ggccaagggt tccaatcacg ccgcttcctt
    37561 cttggcatga tcggctatgt caaaaccttc caccccgaaa ttgccgaaca tctcaccaag
    37621 cagcatgaag ccggtcgcat tgtgctgccc gggcagctga ctccgccgcc tcaggaaccc
    37681 gcaccccatg tccatagccc ggagtgccgt catcaccacc actgatcacc tgccgctcgc
    37741 ccaaccatga gagacgccca ttcgccgttt gagcaggttg tctaccgcta gattccccct
    37801 tcgctttccc tcgttttccc tttagttaca ccccgtttct gccgatacag tcgggccagg
    37861 atttacacct gcgcaagaca caaatccgga ctgcgcgcgc aacgcattga aatttcatgc
    37921 attcatttgt cagtaatctc ttagccgcct tgccgaaggg agatgcgctg gacgacccgg
    37981 cctggtccgt ccgtcaccgc ggcattctct atatcctctg gttgcacgtt cttggcgttc
    38041 ccctctacgg cgcctatatg ggcgccgggc ccagccttta tctcggcggt ggagccctcc
    38101 tcggcgccat tgcgatcgcc gcacagcttc cggcagtcgg tcgtcggctt caggccgcca
    38161 ttgccaccta tggattggtc acagcctcgg cgctcttggt gcacctctcc agcggacgca
    38221 tcgaactgca ctttcatttt tttgtgatga tgtccgtgat cgtgctgtac caggattggc
    38281 tcccgtttct ggttggactg cagttcatcg tcatcgacca tgggattctc ggcaccctgg
    38341 tgcccagcat ggtgtatgtc catgccgaag ggcaggcgca cccgtggacc tgggcactga
    38401 ttcacggcag cttcattctg gctcaatgcg cagcgctcct ctatttttgg cgtgtcaacg
    38461 aggtcgctcg cgaggaagcc ctgcagagtg aggcccgcac ccggatgatc atcgaaaccg
    38521 ccctcgacgc cgtcgtcacg acgaacgtct ccggcatcat taccgactgg aacacccagg
    38581 ctgaattgat gttcgacgta tcccgcaccg acgccatcgg acaacccctg accacctatc
    38641 tctccacggc ccactcttca accgcggccg atgccggcgt ggtgctttcc ggactggttc
    38701 ccggagcgat tctcaaccgt cgagtggaga gcatcggccg gaccggttcc ggccaggaat
    38761 ttcccgtcga gatcgccacc agctgcctcg ccatcggagg aactcaacag ttcaccacct
    38821 tcatccagaa tatttccgaa cgcaagcagc acgaggaagc cttatgccgg gccaaggagg
    38881 cggcggaatc ggccagtcag gcgaagtccc aattcctggc taatatgagt cacgagatcc
    38941 gaaccccgat gaacggggta ctcggcatga cggaattact cctcgcgacg cagctcaacg
    39001 accggcaacg acgatatgcc gacaccgcgc acacatccgg gaccaactta ctgcacatca
    39061 tcaatgacat cctggatttt tcgaagatcg aagccggacg cttggtcttg gagcacatcc
    39121 cctacgactt gcgccaactg ctggccgaaa ccgcgaccat ctttcacgag caggcaggga
    39181 aaaaagggct gtcgttgacc ctggcgattg cgcaagacat ccccatgacc ctccacggag
    39241 acccgcaccg cctgcggcag atcctcacga acctcatcag caatgcgctc aaattcacaa
    39301 ccaccggacg gattgccatc tcggcagcca tggatcaggc ccggcctgaa catgtacgga
    39361 tcgaggtgtc cgacacggga atcggcattc cagaagacgc tcaggctcga atcttcgatt
    39421 catttgcgca agctgacgga tcgactacga gaaaatatgg cgggaccggg ctcggcctcg
    39481 ccattgtgaa gcaattagtc ggcatgatgg gcgggcatat tgaagtgacc agtgtgcccg
    39541 gagagggagc caccttctgg tttacgatcc tcgcgtatgc caccgcagca cgatcgactg
    39601 cctccgatcc atccacctct tccacggagg cgctgcggcg cctgcaccat aagtaaggag
    39661 aacatcccat gcatgtcgca accacctctc tgaaagccgg tcatgccggt attcctgctg
    39721 aactggccgc tcacgattcg gagaacagtc ttctcctgct gttcggatcc tcaagtctca
    39781 ttgatcgacc ggagctcatt cagcaagtga tggccgcttg ccctcgaagc catgtgatgg
    39841 gctgctctac ggcgggagaa attcatggtg ttgaaatctc ggatgacagc ctggtcgtcg
    39901 cggccgtgcg ctttgagaag acgcgcctcc tcaccaccca ggcggcggtt cacaccccca
    39961 aagattcgta cgcggccgga cgcgccattg ccgcgagcct tcatcagccc tcactcagag
    40021 gcgtgttagt gctctctgat gggttgaatg tcaatggcag cgaactcgtg aagggactga
    40081 atgacaccct gggaggctcc gttgtcgtga ccgggggttt ggccggcgat ggaaccgact
    40141 tcaagcggac atgggtcatc aaagaccgtg cgccgcagag cgggtatgtc accgccatcg
    40201 gcttctatgg cgaccatgtc cgcttgggcc atggctcaaa gggcggatgg gataagttcg
    40261 gccctgagcg gcgggtgact aaatcggcag gcaacattct ctacgaactc gatgggcgcc
    40321 ctgccctgca actctataaa gagtatctcg gtgaccgagc cgccgggctc ccggccaccg
    40381 gcctgctctt ccccctcgcc atcagatctt cgaacaccga tgggaaagtc ctggtccgga
    40441 ccatcctggc tgtcgatgaa gcggcacaat ccatgacctt cgcgggggat ttgccggagg
    40501 gcatgctcgc ccagttgatg cgtgccaact ttgaccgctt gattcaggga gcttctgaag
    40561 cggcaaccct caccctgaac aatcatgaac ctgcgggcag ctcttccccc acgctgtcga
    40621 tcgcgatcag ctgcgtcgga cggcggttgg tgctcgggga acgaacggaa gaagaaatcg
    40681 aagcgacgct ggaaattctg cccaatggaa gccgccaagt cggattctat tcctatgggg
    40741 aaatttctcc ctatgccagc ggagcctgcg atcttcacaa tcaaaccatg acgttgacca
    40801 cgattaccga attgtaatct gtatgcatcc gctgctcaca cgacaactca agcggttggg
    40861 actggagact accgccgccc ccacctcgct tgaggcctgg cagcaactgc ttgaacgcgt
    40921 cagccggagc tacatcgagt ccgaccaggg acgcgaactg ctcgaacgct cgattgccct
    40981 gtccgccagc gagatgcagg aactgaacga gcagcttcgc cgcacatcag aaagtcagtt
    41041 gacccaggag cgggacaaac tccacaccgt gcttcgttcc atgggtgacg gactgtgcgt
    41101 cgtggatcga aactgggaca tcgtcctgct caatcctgag gggcagcgtt tgtggggact
    41161 tcccgaagcg gacgtcgtgg gacgacgcct gcccgatatg atttccctct cgtacggcac
    41221 caatctcacc gagccgatct ttacccagat gctgctggat gaaacggctc aaggacattc
    41281 cttcagaacc gatgatggca tactcacgtc gatcacgggc cgttcgtttc ccgtttcctg
    41341 cgtgttggct cctatcgtgc gggacggtca ggccggtgga gcggtgctgg tctttcggga
    41401 catttcggag cgcaagcagg ccgaggatgt tcgtcgccat acggagagtt tgttgcggca
    41461 acatcaggcc gcactgtttg gtctaaccag caacagcatc attcaaagcg gcgtgctcga
    41521 gccggccctc cgagaaatca cccgggtgac agccgcgacc ctcggggtgc gccggtgcag
    41581 catctggctt ctgcgagacg accatagcgc cattatctgt aaagatctcc acgacgcgtc
    41641 caacaacagc cattcatgcg ggatggaact cctcgccgcg caattcccca agtatttccg
    41701 ggagctcctc acggaacggg tcatagacgc ctcggacgcg cggaccgatc cccgcacgtc
    41761 cgaattcacc gacacctacc tgaccccgtt agggatcagc tcgttgctcg atatcccgat
    41821 tcgcttcaaa gggaaactgg taggagtgct gtgcaatgaa cacgtaggca cgccacgctc
    41881 ctggatgctc gaggaacaac aattcagtta tgccgtcgcc tcgttagtgt ccttggcgct
    41941 agaagcggtc gagcggctcc acgccgaagg ggcgctgcgc aaaagcgaag ggcggaccag
    42001 gctgattatc gacacggcgc tcagctcagt catcagcatc gacgagcagg gacgcattat
    42061 cggctggaac acgcaggcgg agcagacatt cgggtggacg cgccaggaag ccatcggtcg
    42121 cttgatggca gagaccatta ttccggcctc tcagcgagag gctcaccaga gagggataag
    42181 gcagttcctc aaaaccggcg agggaccgat cctcaataaa cgaatcgaaa ttagcgcgct
    42241 gcgcagagat ggcacggaat ttccgatcga actcgcgatc accccgctcc gtctggaaaa
    42301 tgcctatacc ttcacagcct tcgtcgtgga tatttccgag cggaaacagg cggaggaagc
    42361 cctgcgcacc agcgaggccc gcctcacaat gacggtgcaa gggtctcaca tcggcatctg
    42421 ggattggaat ctcaccaccg atgcagtcta tttttcgccg cagtggaaga gtcagctcgg
    42481 ccacgacgat accacattgc ccaacgcatt ccaggagtgg caatcccgca ttcacccgga
    42541 agaccgggcg ctggtccatc agaccgtcca ctcttgcctg aacggcgacc gcgcccaatt
    42601 cgaactcgag catcgcctgc ggcatcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42721 nnnnnnnaag cagcgccatc tcgcgtcgac cgtgcaccgg tcgggacgaa cgctcctcgc
    42781 catcattaac gatattctcg atttctcaaa aatcgaagcc ggcaaactgg atctggaatg
    42841 tgtcggattc gatctgtcgc aacttctcga cgaatccctc gagctgttca tggaagccgc
    42901 ccggcggaaa caccttcacc tcactcaaca ggtcgatgaa cgggtcccgc gttatttgat
    42961 gggggatccg gtccgactcc gtcaaattct catgaatctg ttgagcaacg ccatgaagtt
    43021 taccgaaacc ggcggggtgt cgctgacggc cgactacatg agtggcactc ccactcatgc
    43081 gcaactccgc atttcggtca cggacaccgg catcggtatt ccgcccgcag ccaaattgcg
    43141 aatttttgat gcctttgcgc aagcggacgg ttcgaccacc cgacggtacg gtggaacagg
    43201 tctcggtttg tcgatcgcca aacaattggt gggcctcatg ggaggagcga ttacggtgga
    43261 aagtgaaccg ggccgtggct cgacattctc attcacggcc cgattcgaac tgcaaccgct
    43321 tggcacggga acggacaacg gcctgccatc ctatgagcga cttgctaagc cgcaccttac
    43381 tctcgaaccg ccctatgggc cgtcggagcc atacccgtca tcgcctggaa cggaggccaa
    43441 gagaacggga caccgtgtgg ccaggattct catagctgaa gatagcccgg tgaatcgtga
    43501 agtcgccgtc ggcatgctgg aaatcctcgg gtaccaggtc gagatggcgg aaaacggccg
    43561 gcaggcattg gaattggccg ggcgcagtca gtttgacctc gtgctcatgg attgtcagat
    43621 gcctgagatg gatgggttta ccgcgacggg agaaattcgc cgacgtgaag cggcatccgg
    43681 ccagcgaaga ctcaccatca ttgccttgac cgccaatgcc atgcaaggag accgggaaca
    43741 atgtctcgcg gccggcatgg atgactatct gaccaaaccc tacacgcagg tgcaattgca
    43801 gaccgtcgtg cagaaatggt tagacaagca agggactgat cgagcgctcg ccccagtctt
    43861 cagctcaacg caggcggtat ctggcgaatc agcccccgct gcagctgccc ccccaccaag
    43921 cactccgagc atggatccta aagctctcga cggcattcgg gccttgcaac ggccaaaccg
    43981 gcctgatgtg ctggcgtccg ttcttcgcaa gtatctcgat aactcacgag acagtgtaga
    44041 tgccttacag gacgcgatac gtgcgaacga tcccgctggc ttgcaagcag tggcgcatcg
    44101 cctcaaatcg agcagcgctc agcttggagc ccttgcggta gccgcccgct gtaaggaact
    44161 ggaggcgctg ggcgctcaaa aaaatctgat cgaagccgat cgtattttgg tcgaattaca
    44221 atccgaatat gctgccgctt gtgccgtctt tcgaaacgag atcgccaagg agaaatcatc
    44281 atgaacccgc tcacctcggg acggtcaccc tacgcattag tcaccgacga cgacatcatc
    44341 atccgcatgt ttgcacggga ggccctggaa caagcaggat gggaggtcga agaggctgag
    44401 aacggactcg aagcctgcgc ggcctttaag aagtgcccgc cggatgtcgt actgcttgat
    44461 gtcatgatgc cggaaatgga cgggtttcag acctgtgccg ccttacgaca gttaccaggc
    44521 ggcgaacaca ccccgattct gatcatgact ggtttggacg attttgaatc cattacaaaa
    44581 gcctatgatg cgggcgcgac ggattttatc gtgaagccct tgaacgccat gttgctgact
    44641 caccgcatcc ggtatatggt tcgagccaac caggtactcc aggaactccg agccagtcag
    44701 gcgactctga cgcaggctcg cgatgcagcg atggaagggg cccgcctcaa atctgaattc
    44761 cttgcgacca tgagtcatga aatccgcacg ccgatgaacg gcgtactcgg catgaccgac
    44821 tggctgttgg aaaccgaact gacggcggaa cagctggatt gcgcgcaaac gatccggtcc
    44881 tcgggcgatt ccttgatggt catcatcaac gacattctcg atttttccaa aatcgaatcc
    44941 ggcaaactgt ctctcgaatt gctcgacctt gatctgccga agtttgtcga tcgagtcctg
    45001 gcgttattcg cagagcgagc acaacgatag ggtctcctcc ttcattcgca gattgccgac
    45061 gacgtcccgg ctgctttatg cggagatccg acccgactcc aacaaatcct cagcaacctg
    45121 ctggcaaacg ccatcaaatt cagtgatcgt ggaacggtct ctcttaccgt cgatcttgac
    45181 cagcggccgt ctgaacagcg tctcgagttt cccgccacca gttatggcga actgtcatcc
    45241 actgccagcc gaatcagtca cgtgcgattc tccgtagcgg ataacggtat cggaattgcg
    45301 cctaacgcgt ttgcgaagtt gtttcagccc ttcgtccagg cagacggatc caccacgagg
    45361 aagtatggag gaacgggatt gggtctcgcg atttgcaagc aattggtgga actgatgggg
    45421 ggacacatcg gagcggaaag cgaacctgga gcagggtccg tcttccggtt tactgtccct
    45481 cttcagcaac cactcgcgga caacgaacga aacaaatcag ccgcctgaca gcaggcttcc
    45541 tcttcccctt ctgtcgtctc tgcgctcacg attatggaag agacgcctgt gtatcttttc
    45601 agatacctcc tgtaagactt ctctgataca cctacggcct cgacttcttg ggactgttag
    45661 tcatcccatt gaaatcacta ctgaaaagta atggcttaaa ctttgcttta cttcattgcg
    45721 tacacatagt atacaaagcg ggtcgaaagg ggtacctctc cagcgtcagc atgcaaaccc
    45781 ttacccatcc cagctagtag gcaaatccat acacccaagg atgacatctg tgcattgcct
    45841 gacattcgac atcgaagaac attttcaagt ctcacgattt gactcgccga ttcgccgacg
    45901 acactgggga gccttcgaaa gtcgggtgac agccaacacc tgcaaggtgc tggatcttct
    45961 cgcgcgctat gacaccaaag ccaccttttt tgttctggga tgggtcgccg agcgtcaccc
    46021 cggtttgatc aagcagattg ccgaatgcgg acatgaaatt gcgtctcacg gatatgggca
    46081 tgaattagtc acagcccaaa ccccggagtt gtttcgcgct gatgtgagac aggccaaaca
    46141 gatcctggaa gacctgatag gatctcccgt ccaaggatac cgcgcacccg gctttaccat
    46201 tacgcgtgaa acgctgtggg ccttaccaat cctggcagaa gaaggttaca cctacgactc
    46261 cagcatcgtc cctatccggc acgatcattg tgggcttcct ggatctgatc cctggcagca
    46321 tctgaaacaa acctcgtcgg gtcccatctg ggaggttccg ccatccacgg tcaatctcgg
    46381 tggagtgcgg atacccattg caggaggaag ttattttcgg ctgttgccgt tccccgctct
    46441 gtgcggactg atgcggagga tcgaacaaaa aggccggcct ctcgtcatgt attttcatcc
    46501 gtgggaactc gatccgtatc agccacatat ggaaggcccg ttcctttcca aaatgttcca
    46561 ctatttgaat ctggacaaga tggaatttcg catgtccacg gtcatgaaca gcttccggtt
    46621 cggctccatt gctgaacaaa tcgatctttc cccgatcttg cctatcgatc caaccgtctt
    46681 cccttctacc ccagccgaaa tcgcccaaga cgtcgtctga ccctcgtcac cgccaggctt
    46741 tctcaaccag atagcatcgc catctaggcg gttcacgagc ggccacgtgc tatgcaaaag
    46801 taaggattgc actgcctcgg caggttcagt acggtacgca cgtgagggac gagcctatac
    46861 cagcctacaa gggagacgaa gcatgcagaa gagacaggcg aatttatggg gctggtggct
    46921 caggagggtc ggtctgaccg tggcatgttt ctgcgtcgtg ctgctgccta ccacaggttt
    46981 cggtcaatcc atcgaagaca ccgtacagtt catcagcgac atggccaata cacacggctt
    47041 cgtgcgaagt cttagttgcc ggaatccgaa agccgccaaa ccaaccaagg tgaccgaaat
    47101 ctatagtgtc attccaacag gaaccagact cggcacgatc gagttgaacc acgggcacga
    47161 cgacgtcaac accggcttta ctcatttcaa tctgcaggac atcgagagca ttgcctacca
    47221 ggggcaggac acgcgtgaga ataacctggt gctgtatgcg gtccgcatat catgcaatag
    47281 caatggcccc tgcattctga aaaccagctt ttgcaccgga gcggtcacgg aagtgaaagg
    47341 ccagtttcct gaggacacat tgctctttcg ctccgcctca cacgctgagc gcatggccaa
    47401 agccctctct cacttcctga ccctcgtccg cacccagcaa cagaatcaac ccttctaatc
    47461 agaaccgtca gcacccgggc tgatcgtaca aggcagatcg gcccggtgct cacgtccagc
    47521 gcctcactcg ataatcgtca ccgtcatctc gacacgatca atcggattat cgcaagggcc
    47581 cttgtctgca ggactcgggg cacccgtttg gcacatgact ctcgggagtg ccacaatttt
    47641 gtccgcgact ccgatccctt tcaccacttc gccaaagatg gtgtatttcc ggtccaggaa
    47701 acgcgattcc tccacgacga taaaaaattg cgaccccgcg ctatcgggat cctgtgcccg
    47761 cgccatcgag acaacgcctc gcttgtgcgg cagatcgctg aactcagctt tgacgttgtg
    47821 tccgggaccg ccttgaccat aggcgtcttt tctcaacgag tccttggtgt ttgggtcccc
    47881 cccctgaatc ataaacccag gaatcacacg atgaaatatc gtcccgttat aaaatcctga
    47941 cttcgcgagc ttgatgaaat tttcgacatg tttaggcgca acgtccggca aaaacttgat
    48001 ttcaatgtca ccaaccttgg tcttgatgac cgctcgcaca ttactcgcat tgacggaatc
    48061 gcctgccttg gcaggagcat cagccgccag accggttgta gccatgccga tggccagaac
    48121 caggctcatc actgccgcgc acccaaatcg attgtgccat cgtgattgtg tcatcatgcc
    48181 tccttcttgt cggttccttg aagagctcat gtcgttaatt gtgtcagcgc ttcccgcgcg
    48241 gccatctgct ctgcctcttt cttgctgtgc ccgcatccga ctccccgcat cacgccctga
    48301 attgtcagtt ggacctcaaa caccttctca tggtccgggc cggattctcg aaccgtcgca
    48361 tactgcggca aactttcaaa ccgcttctga caaagttcct gtagctgcgt cttgtaatcg
    48421 tccattcccg gccgggcccg ttctgcgcga gccgccagca actcctcttc cagcacacgt
    48481 aaggtaaagg tccggctcgc ctcgaggccg ccatccaggt aaacggcggc gatcaacgcc
    48541 tcgagggcat cggccagcaa cgagtgtttt tcacggcctt tcgaaagctc ctcgcccttt
    48601 cccaaccgca acagacggcc gagcttcatc cgacgggcgg ccttggccag ggacgcctca
    48661 ctgaccaaat gcgccttcag tttggacagc ccgccttcgt tgctgccggg gaattctgcg
    48721 gcgagatact cgctcatgac taacgacagg accgcgtcgc cgagaaattc caagcgctcg
    48781 ttttgagtgc ggtctttggt acgtcgctcg ttcgagtagg atttatgggt cagggcttct
    48841 tccagcagac gcggctgacg aaagcggtag cccaacaggc gctggaccgc ctcgattgac
    48901 gttgccgacg tcataccacc tgactcgtgc tctgaggagg attattggtc acaccggcga
    48961 aaaagcaggc aggcgttgac cccgccgaag ccgaatgaat tcgaaaggac cacctgagtc
    49021 ttggtcgcac gagccttatt cggaacataa tccaagtcgc aggcgggatc aggatgatca
    49081 aggttgatcg tgggaggcag aataccatgg tgcaacgcca gaatactgaa tacggcctcg
    49141 atcccaccag cggcacccag gagatggccc gtcatcgatt tcgtcgaact gaccggaatc
    49201 ttataggcct gctccccaaa cacgtgcttg atcgccttgg tttcgatagc atcggccatt
    49261 gtcgaagtgc cgtgggcgtt gatatagccg atgtccgtct tcgccacacc agcgtctttg
    49321 atcgccattt ccatgcaacg caccgcaccc tcaccttctt cgggcggcgc cgtaatatgg
    49381 taggcatcac tattcatggc atacccgatg acttcggcat agatgcgtgc accgcgcgcg
    49441 cgggcgtgct ccaattcttc gagcaccacc acgccggccc cttctcccaa cacgaatccg
    49501 tcacgatcct tatcgaaagg ccgactggct ttcgccggtt cggtattcct gaacgaaagg
    49561 gccttagccg acgcaaatcc ggcaacaccc agcggggtaa ttgccgcttc cgcaccaccg
    49621 gcaatcatga catccgcatc gtcccgcttg atgaggcgat aggcgtcgcc gatgcaatgg
    49681 ttccccgttg cgcaggccgt gaccgcgcag gaattcggac cttttgcgcc gactcgaatc
    49741 gccacctggc cagaggccag gttgatgatc gtcatcggaa taaaaaatgg cgagactcga
    49801 ccggggccct tttccttcag cacatcatgg taatgttcga tcgatcccag cccgccgatg
    49861 ccagacccaa tatagactcc aaccctcgtc gcctcttcag ccgagacttt caggccggca
    49921 tcgtccactg ccagctgact cgcccccacc gcatagtgga tgaaggtgtc catcttcttg
    49981 atttcttttt tctcgatgaa ctgcgccggg tcgaaatctt tcacctctgc ggcaatctgc
    50041 gcatcgtacc cactcggatc gaatcgcgtg attcggccga cgcctgactc gccggcgcac
    50101 agcgccttcc aggtcttgtc tacgccggtg ccgagaggcg tcaccagtcc aagaccggtc
    50161 actaccacac gtctgacggg tcgatcgtgc atactaccgt ccgttacgct ttctctttga
    50221 tataatccag cgctttgcca acggtgagga tcttttctgc atcctcatcg ggaatttcaa
    50281 tctcgaactc ttcttcgagg gccatcacca gctcaaccgt atcgagcgaa tccgcgccca
    50341 aatcttcaac gaaatgcgcc tccagcgtca cttcttcttc ctcgacccct aactgctcgg
    50401 caataatttt cttcacccgt tcatctactg ttgccatgga cttgcccacc tccttcc
//
LOCUS       DAOQDS010000028        50085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_217_length_50085_cov_18.024745, whole genome shotgun sequence.
ACCESSION   DAOQDS010000028 DAOQDS010000000
VERSION     DAOQDS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 50085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50085
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217_length_50085_cov_18.024745"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /gene="alr"
                     /locus_tag="PK589_08725"
     CDS             complement(<1..970)
                     /gene="alr"
                     /locus_tag="PK589_08725"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNJ19725.1"
                     /translation="MYQPSESSPTSASVDLTALAQNVAHVRRLAPRADVLAVVKANAY
                     GHGAVEITRALQQLAVRRFGVATVDEGVALRQAGIRDDIVVLGATMPAQFPDLVAHRL
                     TPVLYRADLLPAFAATVKTGTAPYPVHIKVETGMGRLGVLPRELGELVSSPELRGPLQ
                     LEGLMTHLADADNPDPEQTEEQLARFQQVLPQVQERGFAIPLIHVANSAGIIKYPKSL
                     HSLVRPGIMLYGYHTLANDRQGPELQPILTWQTTIAHMHTIPPGSSVSYNRTFIASRQ
                     TRVAVLPVGYADGYNRLLSNRGQVLIGGQRVPVIGRVCMDMTMVDVT"
     gene            complement(997..1560)
                     /gene="frr"
                     /locus_tag="PK589_08730"
     CDS             complement(997..1560)
                     /gene="frr"
                     /locus_tag="PK589_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNJ19726.1"
                     /translation="MSTAQEVKQRVTEKMDHAIEHLKRDLAGIRTGRASVALLDGIKV
                     DYYGTLTPLKQVANVATPEARLITIQPWEQNLIREIEKAIQMSDLGLTPSNDGKMIRI
                     PLPPLTEERRKDLIKVCKKHGEEMKVQIRGFRRDGNEELKKLQKDAKLTEDELRKSET
                     EIQKFTDQYVQKIDDMMKKKEGEILEV"
     gene            complement(1572..2297)
                     /gene="pyrH"
                     /locus_tag="PK589_08735"
     CDS             complement(1572..2297)
                     /gene="pyrH"
                     /locus_tag="PK589_08735"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNJ19727.1"
                     /translation="MSSAHYRRILLKVSGEMLAGEQGYGIQPSILEGLAEEIADVVAM
                     DVEVAVVIGGGNIFRGLAASARGMERASADYMGMLATVLNALALQNALEGRGVSTRVQ
                     SAIEMRQLAEGYIRRRAIRHLEKKRVVIFAGGTGNPYFSTDTAASLRAMEIGAQVIMK
                     GTKVDGIYDSDPVKNPQAKKYNEIPFLSILTQNLKVMDSTAISLCMDNRLPLIVFNLK
                     EKGNFKRVVQGDPIGTLVTVESR"
     gene            complement(2294..2890)
                     /gene="tsf"
                     /locus_tag="PK589_08740"
     CDS             complement(2294..2890)
                     /gene="tsf"
                     /locus_tag="PK589_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNJ19728.1"
                     /translation="MASLSELVKELREKTGAGILDCQKALTENGNSIDKAIDYLRQKG
                     LAAAQKKAGRETNQGLIHAYIHAGGKIGVLIEVNCETDFVARNEQFKAFVNDLALQVA
                     AASPSYVRREEIAADVVAKERSIYEGQAKELGKPPAAWPKIVEGKLEKFFQENCLLEQ
                     AFIKDPSVVIKDLLAQQIATIGENMNIRRFTRFQLGQA"
     gene            complement(2942..3715)
                     /gene="rpsB"
                     /locus_tag="PK589_08745"
     CDS             complement(2942..3715)
                     /gene="rpsB"
                     /locus_tag="PK589_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNJ19729.1"
                     /translation="MGVVAIKELLEAGVHFGHQTNRWNPKMKRFLFGERNGVYIIDLQ
                     QTVERMEQAYAFARDTVAAGDSVLFVGTKRQAAEILEEEAKRANQFYINQRWLGGMLT
                     NFQTIRRSIDKMKKMEATLADPSHQGHTKKELGQMQKEVVKLQKNLSGIRNMRALPGA
                     VFILDTRIEHIAILEASRLEIPVIAIVDSNCDPDHIQYPIPGNDDAIRSIKLIISRIA
                     DACLEGAHLRAQQEETEFASAPAGGAAKPAPKLAGVSAS"
     gene            complement(3945..5156)
                     /gene="argJ"
                     /locus_tag="PK589_08750"
     CDS             complement(3945..5156)
                     /gene="argJ"
                     /locus_tag="PK589_08750"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNJ19730.1"
                     /translation="MKTISGGVTAPIGFRAAGVYSGIKKKKKKQLDLALLVSDREGPV
                     AGVFTKNQVVAAPVILDRLHLKQGVGRAFLVNSGNANACTGPEGMQAAKEMAKLAATA
                     LNCAPQTIFVGSTGVIGQPLPIDCIRKALPALSARITRRGGAEAAAAIMTTDLKPKHV
                     AVRALLGGKMVTVGGMAKGSGMIHPNMATMLGYLTTDAAIGQRALQAALRQAVDQSFN
                     CITVDGDTSTNDTVLCLANGMADNRPITPGSADFTRFCAMLETVCRTLALKICWDGEG
                     VTKVVRVEVKGAQSTKAAKQVAQTVATSNLVKTALFGGDANWGRVMAALGRAGVPIDP
                     SRISLQFGGVPMVRKGQGLGRAAERQLTKVFKAKEFTILIDLAQGKAGAHMWTTDLSY
                     EYVRINASYRS"
     gene            complement(5214..6287)
                     /gene="argC"
                     /locus_tag="PK589_08755"
     CDS             complement(5214..6287)
                     /gene="argC"
                     /locus_tag="PK589_08755"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNJ19731.1"
                     /translation="MTSVRVAVAGASGYTGAELVRLLSQHPHVQLTAVTSEKSAGMAI
                     SAVYPHLQGIVQLPFEALAPEALAERADVLFLALPHTKSMGPVASCMKAGKRVIDLSA
                     DFRLKNPATYETWYQTAHAQPQLIKDAVYGLPELHRAAIAQARLVASPGCYPTAAILQ
                     LAPLIAHGLVSPDSIVIDAKSGISGAGRSPALPYHFPEAHESLEPYKIGQHRHIPEIE
                     QELTGLAGSKGRAAEAGQGPVTVAFTPHLVPMNRGILSTAYARMTNQPDVAELRNLYR
                     EFYKGERFVRLLEGAMPNPRHVRGANYCDIAVQTDRRAGWVVTVSAIDNLIKGAAGQA
                     IQAMNLMLGYPEETGLIAPGVYP"
     gene            complement(6394..6786)
                     /gene="rpsI"
                     /locus_tag="PK589_08760"
     CDS             complement(6394..6786)
                     /gene="rpsI"
                     /locus_tag="PK589_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNJ19732.1"
                     /translation="MAAMTQYATGKRKSAIARAWVTAAAGDIVINEKPLEKAFPRPTL
                     RNVIQFPFELAGVAGKYSVRATVYGGGPAGQAGALRHAISKALVIMSAAFRGPIKKEG
                     LLTRDSRVKERKKYGQKGARKRFQYSKR"
     gene            complement(6829..7260)
                     /gene="rplM"
                     /locus_tag="PK589_08765"
     CDS             complement(6829..7260)
                     /gene="rplM"
                     /locus_tag="PK589_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNJ19733.1"
                     /translation="MTKTYLEKPANVQRKWYLVDAEGKTLGRLAAKVATLLRGKHKPT
                     FTPHVDTGDHVVIVNAEKVVLTGNKMENKTYSTHSGFPGGLKTLTAEHVHRKDPTKLL
                     TKAIEGMLPKNPLGNHMAKKLRVYAGATHPHQAQKLESLSL"
     gene            complement(7311..7802)
                     /locus_tag="PK589_08770"
     CDS             complement(7311..7802)
                     /locus_tag="PK589_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNJ19734.1"
                     /translation="MMDAAAPQHDASELVALSVKQVLDDGNTDTRIVVLKSEDASVTL
                     PIWVGSAEGNAIRLAMEHVVTPRPMSHDLIRSFADHLGVRIERIVITDVKSSTYYASI
                     AFASKGLHRTLDARPSDAIALALRAGCPIYATQDVLNRRTAVHLDAWINKLDANNTET
                     QQA"
     gene            complement(7802..8257)
                     /locus_tag="PK589_08775"
     CDS             complement(7802..8257)
                     /locus_tag="PK589_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNJ19735.1"
                     /translation="MITQMRVKGLIFDPYNNAYIVVLRDEENSDMLPIWVGKSEASAI
                     GLALESVTAPRPMTHDLMKAFLETFDAKIISVVITDLNDNTYFATIHLMYEDSEYTVD
                     SRPSDAIALALRTSAPIFASEKVIKKQSSEELEQWLENLKPEDFGKLDS"
     gene            complement(8476..8757)
                     /locus_tag="PK589_08780"
     CDS             complement(8476..8757)
                     /locus_tag="PK589_08780"
                     /inference="COORDINATES: protein motif:HMM:NF016265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HNJ19736.1"
                     /translation="MADVQCVTCGQAGEAITDMLFLGKLEAEIKAKVCKPCWKKWEGM
                     RVMVINEYQVNLGDESGRELVKKQMKAFLKLGEQTDTSKLDQNYRPAGT"
     gene            8934..9020
                     /locus_tag="PK589_08785"
     tRNA            8934..9020
                     /locus_tag="PK589_08785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8968..8970,aa:Leu,seq:caa)
     gene            9297..10079
                     /locus_tag="PK589_08790"
     CDS             9297..10079
                     /locus_tag="PK589_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19737.1"
                     /translation="MRNVLALGAAVLFISSVGVAGAQERLPQSSSGSGFGVGNSVGDV
                     SGNSGRTAAFDRTAFMERLSQPETIYGRVLAIDIPGGKLHLETGGSSHDEGRAGQGAM
                     SSLTTYLDAQTNMDQIRVLNAGDDISLQVVEAPNQRADLGIYGSGRKMVREVYLMRGN
                     EKLAGFGGLGQRPAPSTERGIETNSATNTGGPVGGVFPGTVTSGVITTTVGEFTGTAP
                     CWNCEPQPGWGYQTVAPETKVQNKSDYGTDGAKPNLQKGWTN"
     gene            10231..10491
                     /locus_tag="PK589_08795"
     CDS             10231..10491
                     /locus_tag="PK589_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNJ19738.1"
                     /translation="MSNSSAHVHTPDEELDLRGVICPYNFVKTKLKLESMAPGQLLLV
                     HLDDGDPIRNVPRSVSDDGHDVVSQERAEQSFRVMIRKRADE"
     gene            complement(10496..11245)
                     /locus_tag="PK589_08800"
     CDS             complement(10496..11245)
                     /locus_tag="PK589_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNJ19739.1"
                     /translation="MRRERSQEKCTQSGTGSGTLFIVGVPIGHPDDLTVRALSTLRHV
                     DLIAAKNPQSTQALLSHHGIHAVVTTYDRNNAAEKAPVLLQRLKQGRDIALVSDCGMP
                     AVYDPGRVLISAASKAHVAIEVIPGTSVVVAAAALCGMDGDSFVFEGRWAGGLRHMTH
                     RLHSMRAESRTMIFLPPAKKLPALLSLILHTLGNRRVVIARDLTKDTQTVVRGRVRTV
                     LARDSFHNSRSQITLIVEGMKGKGKTARGPA"
     gene            11311..12117
                     /gene="ccsA"
                     /locus_tag="PK589_08805"
     CDS             11311..12117
                     /gene="ccsA"
                     /locus_tag="PK589_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNJ19740.1"
                     /translation="MSSVFFMLTMGLYFVSTVCYLAYLLRRAETLSKVSLGITAAGFA
                     THTVALVARMADSSQAQLPTFHEALSFFSWMLILVFLAVEFRRQLHVLGSFIVPLALV
                     SLVTAAAFRSDGASSLQPVFKTLWVHVTLSMLGTVGFAIAFVAGVMYLIQDGLLKSKR
                     FNVLYAKLPALDYLDHLNQQSIVMGFPLLTLGIISGAFSAEIVRGTYVNWNPEQTWAM
                     VTWGFYFAVLVGRLTIGWRAKRAAYLTIIGFAGVILTLIGVVLKSHGSVS"
     gene            12117..13523
                     /gene="hemA"
                     /locus_tag="PK589_08810"
     CDS             12117..13523
                     /gene="hemA"
                     /locus_tag="PK589_08810"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNJ19741.1"
                     /translation="MHVIVVGLSHKTAPVEIREKLAVPESRLREALSRLCTYQGVREG
                     LLLSTCNRVEVYAVVEDLESGYGRVQEFLADTHLSLSSEHLTPHLYWHAGDRAISHLF
                     RVASSLDSMIVGESQILGQIKDAFEVALTHKASGLLLNKIVKKAISVAKRVRTETKIA
                     ETAVSVSYAAVELAKKIFSNLTEKTVLLIGAGEMAKLAARHLIANGVRQVRITTRNFQ
                     HGLELAQKFNGTAVPFEDYKTELAGADIVLVSTGASHYLVTADDVQRAMRQRMSRPMF
                     LIDISVPRNIDPAVRPIDDAFLFDIDDLHMRVEQNREDRLREAAKAEQMVVEEVAVLG
                     QWLQSLEVTPTIVALRGRTEEIKRREVDKVLARLAHLSPEDRAAVEALASAIVNKMVH
                     GTMVTLKAEATSSSGAAYVDAARRFFSLEDAPAAYPPAQTEVQEVSEDSSDGNGANRF
                     SEASLVQHTTKEQSRRVS"
     gene            13610..14539
                     /gene="hemC"
                     /locus_tag="PK589_08815"
     CDS             13610..14539
                     /gene="hemC"
                     /locus_tag="PK589_08815"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNJ19742.1"
                     /translation="MSRALILGTRGSKLAVHQSEWVQARLQELVPGLTISLKRIQTSG
                     DKILDVPLAKIGGKGLFVKEIEDALLSGEIDLAVHSMKDVPTALPDGLDILCVPPRED
                     ARDALITRDGCRLDELKPGARIGTSSLRRQSQLLHHRPDFVIEMLRGNLDTRLRKLRE
                     GQFDAIVLAAAGLRRLGWNAEITEYLPVELSLPAIAQGALGIEARRDDSFVRDVLARF
                     EHRPTRITVTAERALLHRLEGGCQVPIAAHALLDGETLKLDALVASVDGKRVIRQHIN
                     GLATEAHQLGTTLAERLLSAGGDVILKEIYGRA"
     gene            14539..16107
                     /gene="cobA"
                     /locus_tag="PK589_08820"
     CDS             14539..16107
                     /gene="cobA"
                     /locus_tag="PK589_08820"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNJ19743.1"
                     /translation="MVKTTRKGTVYLVGAGPGDPKLLTLRGKECLEQADVVLYDYLAN
                     EALLQHVPQTAERLYVGRRGRGHYRDQSEINRVLIDQARDGKCVVRLKGGDPFVFGRG
                     GEEAEAVAAAGLDYEVVPGVTAAVAAPAYAGIPVTHRTLASTVTVITGHEDPTKERSS
                     IEWTRLATVHGTLVFLMGMTNLPKIVQCLRAEGKDGTTPVAVIRWGTRVSQRTVVGTL
                     DDIVEKAAAAHMEPPTVIVVGEVVRLRPQLNWFEQRPLFGKRILVTRPKPQAAEFSDL
                     IQAQGGEAVECPTIEIAPPEDQAPLDAALARLSTYNWLIFTSVNGVGPFMTRLLSTQR
                     DVRALAGLTICCIGPRTAEALAAYGLRADVIPEQFQAEGILDSLAGRHLRGAKVLIPR
                     ALVARELLPEQLRAQGAEVDVVPVYRTILPTVALDHLVGQLGAGEIDVISFTSSSTVR
                     NFVGLFPSKDELLRLIGETTIACIGPITAATAREAGLTVHVMAAENTIPALAEAIVRH
                     VEHQRDRRPASANR"
     gene            16160..16552
                     /locus_tag="PK589_08825"
     CDS             16160..16552
                     /locus_tag="PK589_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ19744.1"
                     /translation="MVPVKSFMVPKDKFITVERDVDVRTAGRVMRDRNIGSLFVTNGK
                     DVIGIITDTDMVRRVVATGADMTKTTVEQIMSAPLVTIEEHKTLLDANDLMAQTHLRH
                     LGVTKDGQLVGMISVRDLVVFLTNLPRK"
     gene            16557..17540
                     /gene="hemB"
                     /locus_tag="PK589_08830"
     CDS             16557..17540
                     /gene="hemB"
                     /locus_tag="PK589_08830"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNJ19745.1"
                     /translation="MAFPMQRMRRTRETEPLRRMVRETTLSPSDFIYPVFVTEGQGRR
                     EPINSMPGQCRVSIDLLIKEALEVKSLGIPAVILFGIPDRKDERGSSGFDPNGIVQRA
                     IRALKEQVPELVVITDVCIDEYTSHGHCGIVRNGKIVNDETLDCLRAMAKTHAEAGAD
                     MVAPSDMMDGRVAAIRDELDRAGFVDIPIMAYAAKFASCFYAPFRDAANSSPQFGDRQ
                     SYQMDPANKREALREIDLDVEEGADIIMVKPAMPYLDVISAARERTLLPIAAYQVSGE
                     YSMIKAAAQAGWLDERRAMMESLLSIKRAGAEIILTYFAKEAAALLQSRHA"
     gene            17537..18499
                     /locus_tag="PK589_08835"
     CDS             17537..18499
                     /locus_tag="PK589_08835"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNJ19746.1"
                     /translation="MKISRGLTPSIARPYSVVTIGNFDGHHHGHRALLDRVVSTARRE
                     SGTALVLTFDPHPVKILAPQVNLMFLTTPEEKLARFEAAGIDEVVFLEFTTAFAALSP
                     AQFAKQVLCDGIGTRELFVGEHFAFGKGRAGRIADLLELGAQLGFRVHPTPPVTIDGE
                     VVSSTKIRQMIQAGDLQKAIRFLGRPYGIEGRVIPGAHRGTELGWPTANLRLPEGRVI
                     PPDGVYAARVLWNGRALDSVVYIGTRPTFGAGERLLEVSILDERLELYGETIRVELLG
                     FVREDRVFASAEALSRQIELDVDAARIQLREAALDTVSPLHSQS"
     gene            18503..19987
                     /gene="tilS"
                     /locus_tag="PK589_08840"
     CDS             18503..19987
                     /gene="tilS"
                     /locus_tag="PK589_08840"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNJ19747.1"
                     /translation="MAGPSTATPHPLHNKVIRALRTRALLQPGQSVLVAVSGGPDSVA
                     LLSLLHELAPAWNFSLTVVHCNYGLRGAESDGDASFVAELCRRLKIPCVVKALQIPAR
                     EPGRSSSLQARARELRYQLFRDLVTELGIERVALGHTADDQAETVLLRMLRGAGLRGL
                     GGMPHRRGNLFVRPLLTVTRREILSYLETKSLSFRTDSSNATSVYLRNRVRHELLPVM
                     QSLAPATVPLLARQADIVREDDRLLERLAARRLVRATVNRDSQTLALDRAVLLKEEPA
                     LQRRMLRQAMRSLSPLAIGVRGDVVLSLLASLSAGRSGGSWTVGSLVVTSEQGLVRIA
                     VGDPAGPKTESSSQSASGVALQTVTLSSLPCEVPWPPTGALIRISVVTRERGMALLKK
                     PSSTIAIFDADRLLLPVTVRSWRQGDYFFPAGMAGRRKKLQDYFTDAKLGRSMREHIP
                     LLLSREHIAWIVGQRIDARFAATASTTRFIVARVTHPVRRKGVL"
     gene            19990..20541
                     /gene="hpt"
                     /locus_tag="PK589_08845"
     CDS             19990..20541
                     /gene="hpt"
                     /locus_tag="PK589_08845"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNJ19748.1"
                     /translation="MERIFGRPIVTQEQMRTRIRELGRQIASDYAGKELVLVGVLKGA
                     YAFYADLARAIRIPMRVEFIVVTSYGAGKRSSGKVKLISDLTEPIVGKDVLLVEDIVD
                     SGLTVQYLMKTLSRRKPRSLKVCTLLSKPERRQVEVDLEYVGFKIPNKFVVGYGLDYR
                     QEYRNLPYLAALDQGEEGEQESA"
     gene            20629..22443
                     /gene="ftsH"
                     /locus_tag="PK589_08850"
     CDS             20629..22443
                     /gene="ftsH"
                     /locus_tag="PK589_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNJ19749.1"
                     /translation="MNSRVKNLLFWVVVGLFMILLFNLFSVPTHAPEDEVIFSDFMAK
                     LDKGEVMKVTIKANHISAILKDQSRIRTYTAEYPELVKHLREKDVQIEARPPDESPWY
                     ITFLVTWGPFILFLGLWFFLMRQMQIGGNKALSFGKSRARMLTEERKKVTFSDVAGIE
                     EAKEEVLEIIEFLKDPRKFQKLGGRIPKGVLIVGPPGTGKTLLAKAIAGEAGVPFFSI
                     SGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRLRGAGLGGGHDEREQT
                     LNQLLVEMDGFDTTEGVILIAATNRPDVLDPALLRPGRFDRQVVVNRPDLRGRSEILK
                     VHTKKVPLSTDVELEKIARGTPGFSGADLENLVNEAALWAARLNKKEVELIDFEMAKD
                     KVLMGAERKSMVLTDEEKRTTAYHEAGHALMAKLLPGTDPVHKVTIIPRGRALGVTMQ
                     LPTDDRHNYSKDFLYNNLAILMGGRVAEELVLHDVTTGAGNDLERATDLARKMVCEWG
                     MSEKLGPLTFGRKEEEIFLGRELGSKRDFSEQVALEIDHEVRRLVTENYERAKRILTD
                     NMTSLKALAEALLEKEVLDALEIDQILIQGSSSQTVPA"
     gene            22631..23530
                     /gene="folP"
                     /locus_tag="PK589_08855"
     CDS             22631..23530
                     /gene="folP"
                     /locus_tag="PK589_08855"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNJ19750.1"
                     /translation="MTLDTPSPFRPVVITAGSHNITIQSNPLVMGVLNVTPDSFSDGG
                     AYSTVERAVDHARQMQADGADIIDIGAESSRPGADPVEEGEELRRLIPVVEAVREAVS
                     LPISVDTTKAAVARLAIQAGALIVNDISALQGDPLMAGLVAETGVAVVLMHMQGTPRT
                     MQRSPRYGNVVEEVTVFLRQRAQEAIARGIRPTQIILDPGFGFGKLQEHNLHMLAEFD
                     AFTKLGYPVLAGVSRKQFIGNLIRQPVHEREFGNAGAVAVAVLKGAHIIRVHDVRAMK
                     ETATVVSAISRHARSSSVEVSNA"
     gene            23523..24872
                     /gene="glmM"
                     /locus_tag="PK589_08860"
     CDS             23523..24872
                     /gene="glmM"
                     /locus_tag="PK589_08860"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNJ19751.1"
                     /translation="MRKLFGTDGVRGVANLEPMTSEIAMQLGRAAAHIFMRRAGRHQV
                     VIGKDTRLSGYMLESALTSGICSMGVDVLLVGPLPTPAIAFLTRSLRADAGVVISASH
                     NPYQDNGIKFFSNEGFKLPDEVEARIEQLIISDEIKHLRPTADAIGKAYRIGDAEGRY
                     IEFVKRSLPRDLDFQGIKLVVDCANGAAYKVAPAVLRELGADIEVIANTPDGMNINDH
                     CGAVHPERLQEAVRRNGAHIGIALDGDADRAIFVCEQGHIVDGDHVMAALGLDLHGQG
                     RLASQTVVGTVMSNFGLELAMKKAGIQLMRTPVGDRYLMERMLADGYNFGGEQSGHFI
                     FLDHNTTGDGLISALQILSLMKRTGKPLSELAKAMTAVPQILLNVKVKHKPDLNQIPD
                     IQQAIKTAEATLNGSGRVLVRYSGTEALLRIMVEGERDATIREVADYLADIVRARIG"
     gene            24899..27430
                     /locus_tag="PK589_08865"
     CDS             24899..27430
                     /locus_tag="PK589_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNJ19752.1"
                     /translation="MLPFLTIIFILGIALGSYLQYFPLSIGLFFFASTIAAVLAERRG
                     LLTAGHSSLRLACLFCGCLYWSLFSWSTSHVPVPDRPAALPSTIDGTIVESVRHAPGR
                     LTALMQVTATDDPDLATPFSLRLTWRDPDRDLQRGLHIRLRTRVHAPSGTLNPRGFDY
                     AAYLEAQGVDAVGSVSGAGAIEVLGADEGLLAYPISSRIDAWRSQVRNAADALPQPAR
                     GLFLSLTIGEQGFLAPEVREWFMTTGTVHILSISGSHLGLIALLSFALVRKACLLLPS
                     MLLLGLSRWLIPSRLAALLTLVPVLGYTLLAGAETATIRSAIMIIVGLWTIWLGTPQY
                     LLHALAAAAGLTLLVHPAALYDISFQLSYVSVWVLALALQRQQSIDELPLPQKSLAGR
                     TFYWLQESIRLTAWVTLATLPLVACYFNQVSWMGIFANLLMVPFVGFILLPISLLSAV
                     WVIVSHSSTLPGAGLIELFAQAVTVPTQWLAGLPGAEWFVAAPTVPLMALFYLLGWAM
                     LTDRPAHAPLLVKRAMAFGMACLILWWLWSPRPFTREGQVRITFLDVGQGDSAVIELP
                     KGEVVLIDGGATYERFDMGRSVVAPFLWNRGIRTIDHVIATHPQLDHAGGLAWILAHV
                     QVENFWTNGVTRSEEFWRKIERTLARRHLQAQVAEQGRRLVDDAECDIAVLNPPPGRN
                     ASSAGKQQSLNNLSVVTELTCGGRRMLFTGDIEREGLARMVQSGHLRHIDLLKVPHHG
                     ALSSLDREWLSSVQPDIAVVSAGRRNPYGHPAGEVIAAYESLETQLWRTDQDGAVWAD
                     LDLNKQSLTMHSTREWQLQPALIPIPQWAIEQDNFHRLWRRWNWN"
     gene            27742..28929
                     /locus_tag="PK589_08870"
     CDS             27742..28929
                     /locus_tag="PK589_08870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ19753.1"
                     /translation="MMANRLLFLAPVPPSDHQGGGPLRMLHLLRFLGSRFHVDLVAPA
                     VDGWENAQNSLQGVCAETTFVPPQGPGLLDRLGRVSPYVKDRALASVVRERLASGVYG
                     AVHVEKLGLIASLPTQLAIPVVLDIWSYGLPSPLGIFKSGGGTTGRPKPFVRRLKLGL
                     YDRWCWPLTSCVTVVSEEDRIRCEGAHPGQRVIVVPNGVDCQAIRPKSDHALTSPVLL
                     FSGDMGAEPNVEAATVLATEVFPAIRREFPKAELRLVGRNPDPRVARLAGPGIMVTGA
                     VPDMQVHLRAATIYVAPHFTGPATRTKLLEAMAAGLPIITTSLGTEGMKVQPGRDLLV
                     ADQPADMIESIQTLLASQPDRERFAQAARHMAEVWYDWSRCLWPLEPLYQNLLAPKPA
                     VAC"
     gene            29087..30154
                     /locus_tag="PK589_08875"
     CDS             29087..30154
                     /locus_tag="PK589_08875"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNJ19754.1"
                     /translation="MTLIPHSKPSLGQEELRAVTEVLRSGQITAGPMVAQFERGIAAY
                     IGLHGGVAVSSGTVALELALRALGVGHGDNVILPSYVCSAPWLAVQRVGAQARLVDIN
                     PTTYNLDPQKVRKARTSRTRAVIVPHMFGLPADLTSLQSLDIPLIEDCAQTLGAMEQG
                     RAVGSVGTLTVCSFYATKLLCTGEGGMVLSNDEALLERVRALREYDKAPSLNSAAGNC
                     KLTDLQAAIGVAQLNQLGEFLDKRSALAALYRDMLSPELFTMPVVPAGRTHVYFRFVV
                     RLMNGLQSADDFTDYLSRLAHRGLQCRKPVFRPLHRYLDLPDFPASDEADASAISLPI
                     HPSLTEEAIRQAAHILQEELH"
     gene            30280..31344
                     /gene="mtnA"
                     /locus_tag="PK589_08880"
     CDS             30280..31344
                     /gene="mtnA"
                     /locus_tag="PK589_08880"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNJ19755.1"
                     /translation="MVPTVEWKEGAVRLLDQSRLPSQVEFLDCRDYRSVAQAIRELKV
                     RGAPAIGVTAAMGVALGAQSVDADEYSSFVKQVDAICDHLAASRPTAVNLFWAIARMK
                     QKLASLKGMPIAEIKRELIRESQSILDEDIALCKAMGRHGATVIQNGQTILTHCNAGA
                     LATAGYGTALGVIRAAWEQGKQIRVIADETRPVLQGARLTAWELMQDKIPVTLITDNM
                     AGSLMRQGNIQVCVVGADRIAANGDVANKIGTYSVAVLARAHGIPFYVAAPYSTIDLA
                     TKTGAEIPIEERNPLEVTSIHGSHPVAPSGVAVYNPAFDVTPAELITGIITERGIFKP
                     GELAGAFGTSRPPIKSGNVS"
     gene            31499..31918
                     /gene="ndk"
                     /locus_tag="PK589_08885"
     CDS             31499..31918
                     /gene="ndk"
                     /locus_tag="PK589_08885"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNJ19756.1"
                     /translation="MSERTLAIIKPDAVKKDAIGDIINRYEKAGLKPVAMRLMHLSKA
                     TAQGFYAVHKARPFFDSLCTFMSSGPCVVLVLQGDNAVKKNRELMGATDPAKAENGTI
                     RAAHGANIEFNAVHGSDAPETAKFEIGYFFPEMELVG"
     gene            31950..33470
                     /locus_tag="PK589_08890"
     CDS             31950..33470
                     /locus_tag="PK589_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19757.1"
                     /translation="MQTRRPFSGRRASLPLVILLMAIGSGCAGPPAVVPVAQPIENPV
                     VEAETRLWYQASSAFSDGRYSAATHLYERYLTTYPKSRRANEAHWELAQSYEQMGEAA
                     GALKEYRILAGPEGAPLSTQSAYAERALHRIEALRNQSSLPGARSGHTALYVSFSNLP
                     PMAQVETWIQRLSAQGVSAILMDASLESSFTRPPAPTGPPPSSPLPKLPAGALFSTSQ
                     GPVMTDWFSVMVPQAHELGISVFAVIDLFHAPLSDHRQESRTLLYDPTRRKVHSWTQF
                     DLLAPPVQQEVAQLLADLLRSGVDGMVIRARAENSFAYEVSDGVLRQFEAQVRQPASE
                     VAQALRERMSVRQDAGALASDKTLWRWVGWKSRQELDVLARLKKYLQKAVPRLRMVLE
                     IHPEALSNPTSALVNYGEDAAEARRRGFDLLLGGPSREASDVRTFGGSFKGWSRDVIQ
                     SEKGEPQTLPQGWVLLRTPTEHGGGMFMGLAQQADELRTPDIRHLVFVPDAPPAIP"
     gene            33611..33997
                     /gene="gcvH"
                     /locus_tag="PK589_08895"
     CDS             33611..33997
                     /gene="gcvH"
                     /locus_tag="PK589_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNJ19758.1"
                     /translation="MIPSNLRYHQEHEWVRAEGQQATVGISHFAQDALGDIVFIDLPK
                     VGAKVTAGQQIGEVESTKTTSTIYTPVSGTIAKINTELKDHPEAVNADPYGKGWMAVI
                     DLTTPGEVDKLMTAAQYEEFLSKQKH"
     gene            34086..35513
                     /gene="lpdA"
                     /locus_tag="PK589_08900"
     CDS             34086..35513
                     /gene="lpdA"
                     /locus_tag="PK589_08900"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNJ19759.1"
                     /translation="MSKHLAILGAGPGGYVAAIRAAQLGARVTVVENQALGGVCLNWG
                     CIPSKALLSVVELGDKAKKAKDFGLLLNGPVAYDPAIMVARKNKVVSTLVKGIATLFK
                     TWNIEHVEGTGELVDARTIRVTKPDGTTLQVAADGIIVATGSSWPNLPLFPIDGTQII
                     TSKQALDLTRIPASLLIVGGGVEGCEFASLYSGLGAQVTLVELVPRILPLEDEEISQM
                     MERELKKRGVDIRTGVTVDQIVRQSDVVTAHLRDGLSLNVEQVLVSVGRGFNSRGIGL
                     EKAGVQVGSRGEIVVNERMETNVPGVYAIGDVVGKAMLAHVASQQGKVAVENFMGHAR
                     AIDYDVIPTGIFTLPEIGRVGLTEQQARDRCVGAGKDPQQSVKVGRFRYGGLGKAQAT
                     GDIQGLVKVVVDTESDRILGVHILGAHATDLIHEAALAMHLGATVARAAEMIHAHPTL
                     AEGLMEAMEDVHGHAIHLARKPATT"
     gene            35510..36058
                     /locus_tag="PK589_08905"
     CDS             35510..36058
                     /locus_tag="PK589_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNJ19760.1"
                     /translation="MMQSLLIKVAMLAVTLGSLVWIASVTPVTKVMPGQPQLLPKHSV
                     TTVSSPPPVEVPATRPALASVSQAGTPSRRQVAMPIEESESASARVSEKASTDSPSAR
                     QAGARQVDLNQATLSELEALPGIGPKLAQRVIEHRDENGPFRSVDDLRQVKGIGRKKF
                     DRLRPHVLVTNTKSLARHKGTL"
     gene            36055..37272
                     /gene="tyrS"
                     /locus_tag="PK589_08910"
     CDS             36055..37272
                     /gene="tyrS"
                     /locus_tag="PK589_08910"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNJ19761.1"
                     /translation="MTPLAQQLDLILRGIVEVIQQSELESKLARSIKENRPLRVKAGF
                     DPTAPDLHLGHTVLIHKLKHFQDLGHQVIFLIGDFTGMIGDPTGRSETRVALSKEKVL
                     ENAKTYERQIFKILDPAKTQVEFNSRWMSTMTADGLIELSAHYTVARMLEREDFHKRY
                     TEGTPISIHEFMYPLIQGYDSVALKADIELGGTDQKFNLLMGRDLQRDYGQEAQAVIT
                     MPLLEGTDGVRKMSKSFGNYIALEDQPADMFGKLMSISDTLMHRYYELLTTENLAGVK
                     AAHPMDAKQGLAEQIVARYHGADAGREARTAFQQKFQAREFPEQPDAHVVLTPSDVKD
                     ASAPSIGLVDLIAKTGLVPSKSEARRLIVQGGVEINEQKMTDANGAVPLLVGQLLRLR
                     VGRRKFAVAEYKD"
     gene            complement(37814..38239)
                     /locus_tag="PK589_08915"
     CDS             complement(37814..38239)
                     /locus_tag="PK589_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ19762.1"
                     /translation="MDWQAVRVQQMVSSPQGGVLLRGFGVPDELPHECGRLLLLIEPI
                     IEQGEADSFSSREQIRLTHREGAVARYLAAGLTNKEIANHLHLSEYTVKDHVKRLMRK
                     TQTTTRTATVSRLLLQYPSLSKAKVTDGIMGTIFAKQAV"
     gene            38872..46212
                     /locus_tag="PK589_08920"
     CDS             38872..46212
                     /locus_tag="PK589_08920"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNJ19763.1"
                     /translation="MRSTYQPIRRLLVALAALLLLDPGHGLDAKETDTETPIGAVGGS
                     IHGDPFTGTATASIPIEIPPGRNGVQPGLQLVYSHSNGNGWIGMGWKLELGSIERQSR
                     FGVDYAKNDYTIQMSGMSTELVQAPLPASSDEYRAKFEGSFSKIKMLSNGGFEVTDKK
                     GTKYLFGQSGVSRVSHPTDSSKIYKWLLDRVEDRDGNFMTATYSADQGQEYLSQITYT
                     GNGAAQPGNTIKFYLESRPDGQTLYTANFQVITAQRLKTIEVLSGTTRLRAYKLAYST
                     SASTGRSVLTSVQQFGKDAVLDGSFTVTGGTSLPPVSMTYENESMSLGSDQLWGSKSY
                     GIGCAAASNCRPNFAYVDMNADGRTDFIYQRSDIDEMHVLLSTGSGFGSDTNWGMKSF
                     GIACAGASSCQPAFSYADMNGDGKTDFVYMRSDLDQFRVMLSTGTAFSTDTVWGSKSF
                     GIACAGASNCRPNFAFTDVNGDGKADFVYQRSDIDELRVLLSTGIGFSADTNWGMKSY
                     GIACAGASNCQPNFAFTDLNGDGKADFVYQRSDIDELRVMLSTGTAFGPDTSWGVKAY
                     GIACAGASNCIPNFTFADINGDGKADFVYQRSDIDELRVMLSTGTGFATDTAWGMKAY
                     GIACAGASNCRPAFGYFDVNGDGKADFVYQRSDIDELRILLSTGSAFRPDAVWGMKGF
                     GIACAGAMNCQPSFSYVDTNGDGKGEFAYHRDATDQIRVLPSSPDGILLATMTNGTGG
                     AAITTYAPSTQFSNTLLPYTTRLLTSTVTCDNWGGSSCVGLSSTTTYTYSGGYHHLAE
                     RDFRGFNYAKVTGPVGPTGEQLITETWFHQGNDVAVDTNNPNVANGYTKGMPYRSKVT
                     DATGKVYSETTTTYIADVDGAAPFFTPVAQVDASIENGAKQTRSVFAQYDAYGNVLRE
                     DQYGDLATTADDRTVVRTFANNTTDWLLGFPTSETSYAGIGTTTQVAQTNLYYDGTTS
                     CATASVNQVPTKGHVTRTVRWLNGGTSPETRMAYDAFGNLTCTRDALGQTSTVNYDST
                     NTFVKTATNPLGHVVTTQYYGVDGVLLDKGLYGQVKSLTDSNSQTTTSEYDALGRKSK
                     TTTPDGLTTTVIYNYGPGFTVGTQHVLSSTSGAGLATALTSASYFDGLGRGVKKETPG
                     PDSKTIVTEVQYDSRGAVRKKSLPYFKTLESVTGRWATTSYDALGRVVRMDSPDGTRG
                     LACFTDWVTVTIDAADHRKRETKDAYGRTIRVDEYQGTASTCDTTVGTPYATTTYQYD
                     VQGNLLSVTDARGNVSTMTYDTLNRKTAMHDPDMGNWSYVYDANGNLTKQTDAKAQIL
                     WFRYDALNRRVQKDFTTQKALGAGDVRYTYDGTTNNRKGRLQQVVDASGTVVFQYDGL
                     GRISQTDKTLDGTTYTTQSTYDGLGRLLTVTYPSTPSKTISYAYNGPVLDKVFEGTTT
                     YIQYTNHNALGQAGTTTYGNGVSTTTTYANANNTICSQQNFRLCTLVTNGPGSGGGGG
                     GTSTTYSALADFSGTQGSRGWYYLSSTGTQLTWNGSYWTGTDGYLGLWNDGGHPGNST
                     DAVRRWVAPSAGSIQITGNTFDGDTSCGTDGVVVTIKKNGAVLWTQTIAAGNTTGFTF
                     NLSNSVVVGDQIDFVINKLTTSSCDNTVFTPTIVLTTAGGGSGPLLNGTYTAPASTVN
                     LSTEGTVDWTHWGLSTATSFNHKSGVTSQISNFSAVGGGVAGQYSDNLVGYTWTGGTP
                     TASATNSTTGLYIVGVNKGFQFTVPADTTQRTVKVYVSAWGAQGKMEAFLSDGSAAGY
                     SDTSLSDPGLSSSGAARVYTFTYRAASANQTLTLRWTNLVQNDPAGGNVTLQAATLVT
                     TSGGGSSGTAYQDLRYVYGLNGNVNDIYDNLVPGGAGDQHLSYDGLDRLTLASGPYGT
                     NGATASLTCTYDELGNLTFNSQVGTYAYPASGSSSVRPHAVTTAGSNTYSYDANGNLT
                     SGAGRSLTYNLENKPLTVVISGQTTTFVYDGDGGRVKKIAGTTTTRYISKFYECDNTS
                     CSRMVFAGGQRIATIGASGSIYYYHTDHLGSSSVITDSAGAKAQAVTYFPYGATRTNN
                     STATPAIDVPYKYTGKELDSSTNLYYYEARYYDPTLGRFLSADTLVPNPLDPQSLNRY
                     SYVHNNPFKYTDPTGHFKINVGKFLRRSLGDLGTGIAGVLGQTLGNALYWSGNPILGS
                     LVQGLGTAMLTQSKSGRYVLAGEIIVTTVVLYSVCPGCDGGAAASFIGPMTAAQASAG
                     ATAASVWGGAAAGAIGGAALGGYSAAKNGGDISSGVLFGATIGAASGALGGGLAPAPG
                     MFGTNLHGWLAQTIAKGLITNFGSGAISSYAGGKGNIGNILMGATMNAALGTATTPAS
                     DLFWGQMVPEVMGYLPATDMEPYYINNAEGVPVRGPEPYAPFNTDLGKRLTGMMGDAG
                     ASTFGEVYGEKLGDIIHGRPIHIP"
     gene            complement(46314..47438)
                     /locus_tag="PK589_08925"
                     /pseudo
     CDS             complement(46314..47438)
                     /locus_tag="PK589_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611846.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            47850..>50085
                     /locus_tag="PK589_08930"
     CDS             47850..>50085
                     /locus_tag="PK589_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNJ19764.1"
                     /translation="MCEPAVQPWYGMTALALVVILLTGCASPTLRRAERLAEDGEWDK
                     AVVAYRDALKTDPFDEALKDKLEAAKQQAAVPHYRNGRRALEERRIVDALTEFKAALG
                     LDPTKIEHHAGMADALRLKEANDQLHTADKLLNLGRSEEALTAYERAVELDPHLTQAL
                     THITEITAQQRSTKMFGGSSQPISLRFQNAKLKEVFEIVARTAGLNVIFDKEVRDEPI
                     TIFLKEMAFDDALNLILNTNGMVAQRVAPETLLIMPNNKQKQAQYQDLLMRTYYLNNA
                     KAKDAVNLLRTMLESKHIYVDEKVNAIVVREEPVKLQLAEKLLFAIDRREPEVELDVE
                     VLEVNRTKSLKYGLNYAKQAGTGLVPTGGSGGISTATTQFTYQQLTSIGPQSYLFTLP
                     ASVILDFFKQESDAQTLASPKLRVMNNKAASINVGDKQPILLSTTNVLPGQAATGAVP
                     TTSTVTSIEFKDVGVKLTVEPTINLMNEVMMKIKVEVTRLGDQVTLQAAPEIKQFKFG
                     TRTAETVLMLKDDETVVLAGLIQDEKRKTKSTIPFIGDIPILGDILTSKQDDRVSTEV
                     VLTITPRVVRNMDLPSSAAQAFWSGTENTFATSQLYAPQAIPVSQSSGQSQRPHPAPS
                     SLGSRPGTPTVPPSSGASATDTGVPTGAGSAQEALPPSGLPTVPNEQGIAPVTPGSGG
                     ELVARATGSLVLRPLDVSAAVGQEFRVDLTALHLEQISETSVTVTFDPKLLEFHRVTP
                     GMVAL"
BASE COUNT        10155 a        13734 c        15052 g        11144 t
ORIGIN      
        1 ccgtgacgtc gaccatcgtc atatccatgc agactcgtcc gatcaccggc acgcgttgtc
       61 ctccgatcaa cacctgtcca cgattcgaga gcaaccggtt gtacccgtcg gcatacccga
      121 ccggtagcac cgctacgcgc gtctgtcgcg acgcgatgaa ggttcggttg tagctcacgc
      181 tgctgcccgg tggaatcgtg tgcatgtgcg cgatggtggt ctgccacgta aggatcggtt
      241 gcaattcagg accctgcctg tcgttcgcga gggtatggta gccatagagc atgatgccgg
      301 ggcgcaccag cgagtgcagg ctcttgggat atttgatgat gcccgcgctg ttcgccacgt
      361 gaatcagcgg aatcgcgaag ccgcgttcct gtacctgggg gagcacctgc tgaaagcgcg
      421 cgagttgttc ttctgtctgt tccggatccg gattgtctgc gtcagcgaga tgggtcatga
      481 ggccctcaag ttgcagaggg ccgcgtagtt cagggctgga aacaagttcg cccagttcgc
      541 gcgggagcac acccaggcgt cccattccgg tttcgacttt aatgtggacg gggtagggtg
      601 cagtaccggt cttgaccgtg gccgcaaagg ctgggagcaa gtcggcacga tagaggacgg
      661 gagtcagccg gtgagcgacg agatccggaa attgagccgg catggtcgcg cctaggacga
      721 caatgtcatc acggatgccg gcctggcgga gtgcgacgcc ttcatctacc gtggccacgc
      781 cgaaccgtct cacggccagt tgttgcaagg cacgggtaat ctcgaccgcg ccatgtccgt
      841 acgcgttggc cttgaccacg gccagtacat cagcacgggg agcgagtcgc cgcacgtggg
      901 cgacattctg agcgagggcg gtcagatcga cggaggcgga ggttggggag gattcggacg
      961 gctggtacac gcagtgtctg acaacaggat tgggcgctag acttccagaa tttcgccttc
     1021 ttttttcttc atcatgtcgt cgatcttctg cacatactgg tcggtgaact tttgaatctc
     1081 tgtttccgac tttcggagct catcttcggt gagcttggcg tctttctgca gcttcttcag
     1141 ttcttcattg ccgtctcgac ggaagccacg aatttgcacc ttcatttctt cgccgtgttt
     1201 cttgcagacc ttgatgagat ccttgcggcg ctcttcggtc agcggcggaa gggggatgcg
     1261 aatcatcttg ccgtcgttcg atggcgtcag tccgagatcg gacatttgaa tcgccttttc
     1321 gatttcccgg atcaggttct gttcccacgg ctgaatcgtg atgagccggg cttccggcgt
     1381 ggccacgttg gccacctgtt tcaagggcgt cagcgtgccg taatagtcga ctttgatgcc
     1441 atcaagcagt gcgactgagg cgcgaccggt gcggattccg gccaggtcgc gtttcagatg
     1501 ctcgatcgca tggtccattt tttcggtcac acgctgtttg acttcctgcg ccgtcgacat
     1561 cagtctctcc cttagcggct ttccacggtg accagggtgc cgatcggatc accctggacg
     1621 acacgtttga agttgccctt ttctttcaaa ttgaagacga tcaacggcaa tcggttgtcc
     1681 atgcacaggc tgatggcggt cgaatccatg actttgagat tctgggtcag aatcgagagg
     1741 aagggaatct cattgtactt tttggcttgc ggatttttga ccggatcgct gtcgtaaatg
     1801 ccgtcgacct tggtgccttt catgatgacc tgtgcgccga tttccatcgc cctgagcgac
     1861 gcggcagtgt cggtggagaa ataggggttg cccgttcctc cggcaaaaat cacgacacgt
     1921 ttcttttcca aatggcggat ggcgcgccgg cgaatgtagc cttcggccaa ctgacgcatc
     1981 tcgatggcgg actggacacg ggtactgacg cctcgacctt ccagcgcgtt ttgaagcgca
     2041 agcgcattca gcacggtcgc gagcatgccc atgtaatcgg cggatgcccg ctccataccg
     2101 cgggcgctgg cggcgaggcc gcggaagata ttgccgccgc cgataacgac ggcgacttcg
     2161 acatccatcg cgacgacgtc ggcaatttct tctgccagcc cttcgagaat cgagggttgg
     2221 atgccatagc cctgctcacc ggccagcatt tctccgctga ctttcagcag gatgcggcgg
     2281 tagtgagcag aactcatgct tggcctagtt ggaatcgtgt aaaccggcgg atgttcatgt
     2341 tctcgccgat cgtggcaatt tgttgcgcca ggagatcctt gatgacgacg gagggatcct
     2401 tgatgaacgc ctgttcgagc aggcaattct cttggaagaa cttttcgagt ttgccttcga
     2461 cgatcttcgg ccaggcggcc ggcggcttcc cgagctcctt cgcttgccct tcgtaaatgc
     2521 tccgttcctt ggccaccaca tcggctgcga tctcttcccg tcgcacatag gacgggcttg
     2581 cagcggctac ctgcagggcg aggtcgttga cgaaggcctt gaactgctca ttgcgcgcca
     2641 cgaaatcggt ttcgcagttc acttcgatga gcacgccgat ttttcctccg gcatgaatgt
     2701 acgcgtgaat cagaccctgg ttggtttccc gtccggcctt tttctgcgct gccgccaacc
     2761 ccttctgtcg aaggtagtcg atggccttat cgatgctgtt tccgttttca gttagggctt
     2821 tctggcaatc cagaatgccg gccccggtct tttcacgcaa ctcttttacc agctcactca
     2881 aacttgccat ggcttcctca gttaatcgaa tagaacaatg tcatcgtgcg agatgcagcc
     2941 cttaggatgc ggacacgccg gccaatttgg gcgccggttt ggccgcgccg ccggccggtg
     3001 ccgatgcgaa ctccgtctct tcctgctggg cgcgcagatg ggcgccttca aggcaggcgt
     3061 ccgcaatccg ggagataatc agcttaatgg aacgaatcgc gtcgtcgttg ccgggaatcg
     3121 gatattggat gtgatccgga tcgcagttcg aatcgacgat cgcaatcacg ggaatctcca
     3181 gccggctggc ctcgaggatg gcaatgtgtt cgatgcgcgt atccagaatg aacacggcgc
     3241 cgggcagagc ccgcatgttg cgaatacccg acaagttctt ctgcagtttg acgacttcct
     3301 tctgcatctg tccgagctct tttttcgtat ggccctggtg gctcggatct gcgagggtcg
     3361 cttccatttt tttcattttg tcgatgctgc ggcggatggt ctggaagttt gtcagcatgc
     3421 cgcccagcca gcgctggttg atgtagaact gattggcgcg cttggcttct tcttccaaaa
     3481 tttcggccgc ctggcgtttt gtgccgacga acaggacgga gtcaccggct gccacggtgt
     3541 cgcgagcgaa ggcataggcc tgctccatgc gttcgaccgt ttgttgaagg tcaataatgt
     3601 agacgccgtt gcgctctccg aacagaaacc gcttcatttt cggattccaa cggttggtct
     3661 ggtgtccgaa atggacgccg gcttccagta attccttaat cgcaactact cccatacctt
     3721 cacctccctc cgaagcctgc agagcgcctg gcaaaagccc acggcgaggg gggatgtccc
     3781 ccttattctc aggctgcgca gcgtgcgcat cacgctggtg tggatttaat taagttgtcc
     3841 ctgattttga atgccgcgcg gcgaacgatg ggacaaaccg ttcattgcgc gaaacgcccg
     3901 aacgctagca cagtggccgc gattttgcaa ccgcgcgagg ctccttacga acgataactc
     3961 gcgttgattc gcacgtattc gtaagacaaa tccgtcgtcc acatgtgcgc cccggctttg
     4021 ccttgagcca gatcgatgag aatcgtgaac tctttcgctt tgaagacttt ggtcaactgc
     4081 cgttcggctg cgcggccgag tccttgtccc ttgcgcacca tcgggacccc cccgaattgc
     4141 aggctgatgc gggaagggtc gatgggcacg cctgcacgtc cgagcgccgc catgacgcgg
     4201 ccccaattgg cgtcgccgcc gaagagcgcg gttttgacca gattggaagt tgcgaccgtt
     4261 tgggcgacct gtttggccgc tttcgtggac tgtgctccct tcacttccac ccgtacgacc
     4321 ttcgtcacac cttcgccatc ccagcagatt ttcagcgcga gtgtgcgaca taccgtttca
     4381 agcatcgcgc agaagcgtgt gaaatcggct gagccgggtg tgatagggcg gttgtcggcc
     4441 atgccgttgg ccaggcagag caccgtgtcg ttcgtgctgg tatctccgtc gaccgtgatg
     4501 caattgaatg attgatcgac cgcctgtcgg agcgccgctt gaagggcccg ttgcccgatg
     4561 gccgcgtcgg tggtgaggta gcccagcatc gtggccatgt tgggatgaat catgcccgat
     4621 cccttggcca tgccgccgac agtcaccatt tttccgccga gtagagcccg gacggcaacg
     4681 tgtttcggtt tgaggtcggt ggtcatgatc gccgcggccg cttcggctcc gcccctacgt
     4741 gttatacgtg cagacagggc gggaagcgct tttcggatgc agtcaatcgg gagcggttga
     4801 ccgatgaccc cggtggatcc gacgaaaatg gtctgtggtg cgcaattgag ggccgttgcc
     4861 gccagcttcg ccatctcctt ggctgcctgc atgccttccg ggccggtaca ggcgttggcg
     4921 tttccgctat tgacgaggaa ggcgcggccg acgccctgct tgaggtgcag acgatcgaga
     4981 atgaccggcg ctgctacgac ttgattcttc gtgaagactc cggctaccgg accctcccgg
     5041 tccgacacga gcagtgccag gtcgagttgt ttcttctttt tctttttgat gccgctgtag
     5101 actccggcgg ctcgaaaacc gatgggcgcg gtgacgcctc ctgaaattgt cttcatcgct
     5161 cttcctatgt gatgtgaagg tgactcgcta tgacgggccc atgcaagtgc acgctagggg
     5221 tagacgcccg gagctatgag gccggtttct tccgggtaac ccagcatcag attcatggcc
     5281 tgaatggcct ggccggctgc ccctttgatc aggttatcaa tggccgacac ggtcacgacc
     5341 caaccggcgc gtcgatcggt ttgtacggcg atgtcgcaat agttggctcc tcgcacgtgg
     5401 cgaggattcg gcatggcgcc ttcgagcagg cgcacaaatc gttcgccctt gtaaaactcc
     5461 cgatagagat tccgtaactc ggcaacatcg ggctggttcg tcattcgtgc ataggcggtg
     5521 ctgaggatgc ctcgattcat ggggacgagg tgtggagtga aggccacagt cacagggccc
     5581 tgtccagcct cagccgcacg tcccttgctg ccggctaatc cggtgagttc ctgctcgatt
     5641 tcagggatgt gtcgatgctg cccgatcttg tagggctcca gcgactcgtg ggcttcggga
     5701 aagtggtagg ggagggcggg acttcttcct gcgcccgaga tgccggattt tgcgtcaatc
     5761 acaatgctgt caggcgagac caatccatgg gcgatcaagg gtgcgagttg gagaatcgca
     5821 gccgtggggt aacagccagg tgaggctacg agtcgggcct gtgcaatggc agcgcggtga
     5881 agttccggca gcccgtacac ggcatccttg attaactggg gttgtgcatg tgcggtttgg
     5941 taccaggttt catacgtagc gggatttttc agccggaagt cggcgctcaa atcgatgaca
     6001 cgcttgccgg ccttcatgca gcttgccacg ggacccatcg atttggtgtg ggggagtgcc
     6061 agaaacagga catcggcccg ctccgccaac gcttccggtg ccaacgcttc gaatgggagt
     6121 tgcacgattc cctgcaggtg aggatacacg gcagagatgg ccatgcctgc tgacttttcc
     6181 gaggtgaccg ctgtgagttg tacgtgggga tgttgagaga ggagccgcac tagttctgct
     6241 ccggtatatc cgctggcccc tgctaccgct actcgtaccg acgtcatctt cgcctcccgg
     6301 cccgtgacaa attgtggccc acctgcagcg agccaataaa aaagggaagg ctgcggagcc
     6361 ttcccttttg aaccctgtcg ctccagtcgt tcgttaccgc ttcgagtatt ggaagcgctt
     6421 tctggcgccc ttctggccgt acttcttacg ttctttcacg cgagaatcgc gagtcagaag
     6481 gccttccttc ttgatgggac cacggaacgc cgcgctcatg atgaccagcg ctttcgagat
     6541 ggcgtggcgg agagctccgg cctggcctgc ggggccacca ccgtagacgg tggcgcggac
     6601 ggaatattta cctgccactc cggctaactc gaacggaaat tgaatcacat tccgcagcgt
     6661 ggggcgagga aacgcttttt cgagcggctt ctcgttgatg acaatgtcac cagcggctgc
     6721 ggtgacccat gcgcgcgcga tcgcgctttt tctttttccg gttgcgtact gcgtcattgc
     6781 ggccatatca ggcggtctcc ttcactgatc gaatacaaag aataaaaatc acaacgagag
     6841 tgattccagt ttttgtgctt ggtgcggatg ggtcgcgccg gcatacacgc gcaacttttt
     6901 cgccatgtgg ttaccaagtg gatttttcgg caacatgccc tcgatggctt tcgtcaacag
     6961 cttggtaggg tcttttctgt ggacatgctc ggcagtgagg gtcttcaagc cgccaggaaa
     7021 cccgctgtga gtcgaatagg tcttattctc cattttgttg ccggtgagta cgaccttctc
     7081 cgcattcaca atgacgacat ggtctccggt atcgacatgg ggggtaaacg tcggcttatg
     7141 tttccctcgc agcaaggtgg cgactttcgc cgcaaggcgg ccaagggtct tgccctcggc
     7201 gtccacgagg taccattttc gttgcacgtt agcgggtttc tcgagatacg tttttgtcat
     7261 ggtctccatc tactcctgca cgaaaggatt ctaatgtgag ggatcaggcc tcacgcctgt
     7321 tgagtctcag tattgttggc atccagttta ttgatccatg cgtccaaatg cacggccgtc
     7381 cgccggttca ggacatcctg tgtcgcataa atcgggcacc cggcgcgaag cgcgagggcg
     7441 atggcatcgc tcggtctggc atccaatgtt cgatgaagcc ccttggacgc aaacgcgatg
     7501 ctcgcgtagt acgtactgct tttgacatcg gtaatgacga tccgctcgat tcgaacgccg
     7561 aggtgatccg caaaactccg gatgaggtcg tggctcatcg gtctgggcgt caccacatgc
     7621 tccatggcga ggcgaatggc attgccttct gcagagccca cccagatcgg gagggtcaca
     7681 ctcgcatcct cgcttttaag gacgacgatg cgggtatcgg tgttcccatc gtccaacact
     7741 tgtttgacgg acagtgcgac caactccgac gcatcgtgct gtggtgctgc ggcatccatc
     7801 attagctatc cagtttgccg aagtcttcgg gcttcaggtt ttcaagccac tgttcaagct
     7861 cttcagacga ctgtttttta attaccttct cgctcgcaaa gatcggtgcg ctggttctca
     7921 gggcgagggc gatggcatcg ctggggcgag agtcgacagt gtattcagaa tcttcgtaca
     7981 tcaaatgaat ggtggcgaaa taggtgttgt cgttcagatc tgtaatcacc acgctgatga
     8041 ttttggcatc aaacgtttct aagaatgctt tcatcaaatc gtgggtcatg ggacgcggcg
     8101 cagtcacgct ttccagcgcg aggccgatgg cgctcgcttc cgattttccc acccagatcg
     8161 gcagcatgtc tgaattctct tcgtctcgca gcaccactat ataggcgttg ttataggggt
     8221 cgaaaatcag ccccttgacc cgcatttgtg taatcacggc gggagtatcc ttcccgatgt
     8281 cagccaaaaa ccttaagaag tagggcactc tatcacttct gaaaacagag tgtcaatgaa
     8341 ttcggtgtcg tgacggggtg gcgaggggag gtgtaggcga cgtagacatc cctattagtt
     8401 gaagcagcga tgcgggcaac ggatggcccg aacacctgct tgatcaggca ggtgacgggc
     8461 ggtctccgct gaggtctagg tgccggcggg ccgataattc tgatccagtt tggaggtgtc
     8521 ggtttgttcg cccagcttca ggaacgcctt catctgtttt ttgaccagtt cgcggccgct
     8581 ttcatcgccg agattgactt gatactcgtt gatgaccatc acgcgcatgc cttcccactt
     8641 cttccagcag ggtttgcaga ccttcgcctt gatttccgct tcaagtttgc ccaggaacag
     8701 catatccgta attgcttcgc ctgcctggcc gcaggtgaca cactgcacat ctgccatggt
     8761 tgcctccata caattcgcga attctgattg gatgctaaac gcggtattct agcatccggc
     8821 tcgttgagaa aaaagagtgc gccgcggcga ttgacgagcc tgtgggttcg tgttaaaaat
     8881 gcgtcccctc aaatctgagg atcatgagct gaatgtcgca ggacgagaca gatgcccgga
     8941 tggcggaact ggcagacgcg ccggactcaa aatccggttc tcgcaagaga gtgggagttc
     9001 gaccctccct ctgggcacca ttcgtatcgc ggccaccagt cagctcgaga ctccttccga
     9061 cattttcagt catgccacca ttgttagaac tgaatcgcag tgaaacggga atgaattggg
     9121 ctgtgtcact gacgacaaca ccatatagtg gatgtcacgg ccgaaaacct cttgacatga
     9181 tgtgggggtt agcctatagt tcgcccgaat ttgctgtgga atacttatga ctcagttagt
     9241 ttattgtcgt ccatcatcaa tctcttatca attccaactc actaaggagg cagcacatgc
     9301 gtaacgtgtt ggcacttgga gctgcggtgc tttttatcag ctccgttggt gttgcaggcg
     9361 cccaagagcg cttaccacaa tcctcatcag gatcagggtt cggtgtcggc aactcagtcg
     9421 gagacgtgtc gggcaactcc ggccgcacgg cagcgtttga ccggaccgcc ttcatggagc
     9481 gtctctcgca gccagagacc atctatgggc gtgtcttggc catcgatatc ccgggtggaa
     9541 agcttcacct cgagacgggt ggcagctctc atgatgaagg ccgcgcaggt cagggcgcga
     9601 tgagctcgct cacgacctac ctcgatgccc agacgaacat ggaccagatc cgagtgctga
     9661 atgccggcga cgacatttcc ttgcaggttg tcgaagcccc caaccagcgc gctgacctcg
     9721 gcatctatgg atcgggccgc aagatggttc gtgaagtgta tttgatgcgt ggcaacgaaa
     9781 agttggcagg atttggtggt cttggtcagc gtccggctcc gtccacggag cgcggcatcg
     9841 agaccaactc agccacgaac acgggtggcc cggtgggtgg agtgttcccc ggaacggtca
     9901 cctctggcgt catcaccacc accgtgggtg agttcactgg aaccgcaccc tgctggaatt
     9961 gtgagccgca gccgggatgg ggttatcaga cggtcgctcc tgagaccaaa gttcagaaca
    10021 agtctgacta cggcaccgat ggtgccaagc ccaacctgca aaagggctgg accaactaag
    10081 atttaggcca tctgaatgga cgatggttga gcggggtgct tcgcaagagg caccccgctt
    10141 tgcttttctg tcatgtgata tgcttcctca ccgttgcgcc agcagagtag tcttgatacg
    10201 attcccgatc acttctcact gagagcgtat atgtccaact catcagcaca cgttcacacg
    10261 cccgatgaag aattggatct tcgtggggtc atttgtcctt acaatttcgt gaagacgaag
    10321 ctcaaactcg aaagcatggc tcccgggcaa ttgctcctcg tgcatctgga cgatggcgac
    10381 ccgattcgca atgtgcctcg cagtgtcagc gatgatgggc atgatgtggt ttcgcaagaa
    10441 cgtgccgaac agtcttttcg tgtcatgata cgaaagcgtg ccgacgaata aggcgtcatg
    10501 ccggtccccg tgctgtcttg ccttttcctt tcatgccttc cacaattaac gtgatctgcg
    10561 atcgactgtt gtgaaaggaa tcccttgcca ggacagtgcg gacgcgtcct ctcacgactg
    10621 tctgcgtgtc tttcgtgagg tctcgcgcta tcaccactcg gcgattgccg agtgtgtgca
    10681 ggatgaggga aagcagcgcg ggaagctttt ttgccggagg gagaaaaatc atggtgcgcg
    10741 attcggcgcg cattgagtga aggcgatgcg tcatgtgacg cagaccgccg gcccaacgtc
    10801 cttcgaacac aaatgagtct ccgtccatac cacacagtgc tgctgccgca accacgacag
    10861 acgttcctgg aatcacttca atagcaacgt gtgccttgga tgccgcggag atcaacactc
    10921 tgccgggatc ataaacggca ggcattccgc agtctgacac cagcgcgata tcgcggcctt
    10981 gtttgagacg ctgcagcaac actggcgctt tctcagcagc attattccga tcataggtgg
    11041 tgacaacagc gtgaatgccg tgatgcgaaa gcagcgcttg ggtagactga ggattcttgg
    11101 cagcgatcaa atcgacgtgc cgaagcgtcg aaagcgcgcg aaccgtgaga tcgtcgggat
    11161 gtccgatcgg gactcccaca atgaacaacg ttcccgaccc ggtgccggat tgagtgcact
    11221 tttcttgact ccgttctcgt cgcatcgata taatttccag ttcccgaaaa aagtagcttt
    11281 gcgctgaaat gttgcgagga taggggcagt atgtcgtcgg tttttttcat gctcacgatg
    11341 ggtttgtatt tcgtgagcac ggtctgttac ttggcctatt tgctccgccg tgccgaaact
    11401 ctctcaaagg tttcgctggg aattactgct gccggctttg cgacgcatac cgtcgcgctg
    11461 gtcgcccgga tggctgattc cagccaggcc cagttgccga cctttcacga agccctctca
    11521 ttcttttctt ggatgctcat tttggttttt cttgctgtag agttccggcg gcaactgcat
    11581 gtgttgggtt cctttatcgt cccgctggcg ctggtctctc ttgtcacggc tgccgcattt
    11641 cgttcggatg gtgcgtctag tcttcaaccg gtattcaaaa cgctgtgggt gcatgtcacc
    11701 ctcagtatgc tgggaactgt gggttttgcc atcgcctttg tcgcgggggt gatgtatctc
    11761 attcaggatg gacttctcaa gtcaaagcgc ttcaacgtcc tctacgcaaa attgcccgct
    11821 ctggactatc tcgaccatct caatcagcaa tcgatcgtga tggggtttcc tctcttgacc
    11881 ctgggaatca ttagcggcgc gttttcggct gagattgtcc ggggaaccta tgtgaattgg
    11941 aaccctgagc aaacctgggc catggtgacg tggggcttct atttcgccgt gctggtcggg
    12001 cgcttgacca ttggttggcg ggctaaacgg gcagcctatc tcaccatcat cggatttgcc
    12061 ggcgtcattc tgaccctgat cggcgtggtg ttgaaaagtc acggttcggt gtcctgatgc
    12121 atgtgattgt tgtcgggtta agccataaga ctgccccggt cgagatccgc gagaagctcg
    12181 ccgttccgga aagtcgcctg cgtgaagcgc tcagtcggct gtgcacctat cagggggtgc
    12241 gcgagggatt gttgctctcc acctgtaacc gcgtcgaagt gtacgccgtc gtcgaagact
    12301 tggaaagcgg ctatgggcgg gtacaggaat ttctggcgga tacgcatctc tccctttcgt
    12361 cggaacatct gactccgcat ctctactggc atgccggcga tcgtgccatc agccatctct
    12421 tccgtgttgc gtccagcctc gattccatga tcgtcggtga gtcccagatt ctcggacaaa
    12481 tcaaggatgc attcgaagtg gcgctcacgc acaaggcctc ggggttgttg ctgaacaaga
    12541 tcgtcaagaa ggccatttcg gtggccaaac gcgtgcgtac cgaaacgaaa attgctgaga
    12601 ccgccgtctc cgtcagttac gcggctgtcg aattggccaa aaagattttc tccaacctga
    12661 ccgaaaaaac cgtgttgctg atcggggcag gggaaatggc gaagttagcg gctcgtcatc
    12721 tgatcgccaa cggggttcgt caggtgcgaa tcaccacccg caacttccaa catgggttgg
    12781 agttggccca gaagtttaat ggcaccgcag ttccgttcga ggactacaaa acagaattgg
    12841 ccggggcgga tatcgtactc gtatcgaccg gcgcgtccca ttacctggtc acggccgacg
    12901 atgtgcagcg ggccatgcga caacgaatga gtcgtcccat gtttttgatc gatatctcgg
    12961 tgccgcgcaa cattgatccc gcggtgaggc cgattgacga cgcgttcctc ttcgacatcg
    13021 acgatctcca tatgcgggtc gaacaaaacc gggaagaccg tctgcgcgaa gcggcgaaag
    13081 ccgagcagat ggtggtggag gaagtggcgg tgctcgggca atggctgcaa tcgttggaag
    13141 tgacgccgac catcgtcgcc ttgcgtggcc ggaccgaaga aattaaacgg cgcgaagtgg
    13201 ataaagtcct tgcgcggttg gcgcatttgt ctccggagga tcgagccgcc gtggaagcac
    13261 tcgcgtcagc cattgtgaat aaaatggtgc atggcacgat ggtgacgttg aaagccgagg
    13321 ccacgtcttc cagtggcgcg gcctatgtcg atgcggctcg ccggtttttt agccttgagg
    13381 acgcacctgc tgcgtacccc ccggcgcaga ccgaggtgca ggaggtatcc gaagacagca
    13441 gcgatgggaa cggcgccaac cgattttccg aggcgtcatt ggtacagcat acaacgaagg
    13501 agcaaagccg gcgagtgagt tgacccgcat cgccgtgatc ccccatgagc agattattcg
    13561 ttaccgccgc tccaaccttg atgaccagcc gatccgattg tgagttccga tgagtcgcgc
    13621 gcttattctc gggacacgtg gcagcaagtt agccgtgcat caaagcgagt gggttcaggc
    13681 gcgtcttcag gaactggtcc ccggtctcac catctctctc aagcgcatcc aaacctcggg
    13741 cgacaaaatc cttgatgtgc cgctggccaa aatcggcggc aaggggttgt tcgtcaaaga
    13801 aatcgaagat gcgctcttga gcggcgagat cgatctcgcc gtccatagca tgaaggacgt
    13861 tccgacggca ttacctgacg gcttggacat tctctgcgtg cctccgcgtg aagatgcgcg
    13921 ggatgcgctg atcacgcgag atgggtgccg gctcgatgag ttgaagcccg gtgcccgcat
    13981 cggcaccagc agtctccggc ggcagtccca gttgctgcac catcgtcctg attttgtcat
    14041 tgagatgttg cgcggcaatc tggacacgcg tctcaggaaa ttacgcgaag gacagtttga
    14101 tgccatcgtc ttggctgctg cgggtttgcg gcggctgggg tggaacgcag agattacaga
    14161 gtatcttccg gtggagctca gcttgcccgc cattgcccaa ggggcgctgg gcatcgaggc
    14221 gcgcagggac gacagcttcg tccgcgatgt gctcgctcga tttgagcatc gtccgactcg
    14281 aatcaccgtg acagccgaac gggccttgtt gcaccggctc gaaggtggct gccaggttcc
    14341 gatcgccgcg cacgcgctgc tcgatgggga gactttgaag ctggacgccc ttgtcgccag
    14401 cgtggatgga aagcgggtga tcaggcagca tatcaacggc ctggccacag aggcccatca
    14461 gcttggaacc actcttgcgg aacggctctt gtcggctggt ggagacgtga ttctcaagga
    14521 gatctacgga cgggcgtaat ggtcaagacg acgcgcaaag gaaccgtcta tttggtgggc
    14581 gccggtcccg gtgatccaaa actgctgaca ttgcgcggca aggagtgtct ggagcaggcc
    14641 gatgtcgtct tgtacgatta cctcgcgaac gaagcgttgt tgcagcacgt gccccagacg
    14701 gccgagcgac tgtacgtcgg tcggcgtggt cgaggccact atcgtgatca atcggagatc
    14761 aaccgtgtgt tgattgatca ggcccgtgac ggaaaatgtg tggtccgtct gaagggtggc
    14821 gatccgtttg tcttcgggcg gggtggcgaa gaggccgagg ctgtggccgc ggctggtttg
    14881 gattacgaag tagtgccggg tgtgaccgca gccgtggcag ccccagccta tgccgggatt
    14941 cccgtcacgc atcggaccct cgcatcgacc gtgaccgtca tcaccggcca tgaagacccg
    15001 acgaaagagc gcagcagcat cgaatggact cgcttggcga ccgtccacgg taccctggtc
    15061 tttctcatgg gcatgaccaa tttgccgaag atcgtgcagt gcctgcgggc tgagggcaag
    15121 gatggcacga cgccggtggc cgtgatccgg tggggaacgc gtgtatcgca gcggacggtt
    15181 gtaggcacct tggatgatat tgtggagaaa gcggcggctg cgcatatgga accgccgacc
    15241 gtgatcgtgg tcggagaggt tgtgcgcctc cggccgcagt taaactggtt tgagcaacgt
    15301 ccgctatttg gaaaacggat tctcgtcacg agaccgaagc cgcaagccgc cgaattttcc
    15361 gatctgatcc aggctcaggg cggggaggcg gtggaatgcc ccactatcga aattgcgccc
    15421 cccgaagacc aggcgcctct ggatgcagcc cttgcgcgat tgtctaccta caactggtta
    15481 atcttcacca gtgtgaacgg cgtagggcca ttcatgacgc gcctgctcag cacgcagcgt
    15541 gatgtccgtg cattggccgg tctcaccata tgttgcatcg ggccgcgtac cgcggaggcg
    15601 ctggccgcct atggtttgcg cgcggatgtg attccggaac aatttcaggc ggaaggcatt
    15661 ctggattcgt tggccgggcg acaccttcgc ggcgccaagg tgctgattcc tcgcgcgctg
    15721 gtggctcggg agctgctccc ggagcagttg cgtgcacagg gggcggaggt cgatgtggtg
    15781 cccgtctatc gcaccattct tccaaccgtc gcgctggatc acctggttgg tcagctcgga
    15841 gccggtgaga ttgatgtgat ttcgtttacc agttcgtcga cggtacgaaa ttttgtcgga
    15901 ttgtttccct cgaaggatga attgttgcga ctcatcgggg aaacgacgat cgcctgcatc
    15961 ggtcctatca cggcggccac cgcccgggaa gccggcctga cggtgcatgt gatggcggcg
    16021 gagaacacca tccctgcgct ggcggaggct atcgttcggc atgttgagca tcagcgtgat
    16081 cgccggcccg cctcggcgaa tcgatgatcg tggaaagttg atcggcaaga tgtgcgttac
    16141 ttaacaggga ggatgccgca tggttccagt gaaatcgttt atggttccga aagacaagtt
    16201 catcactgtg gagcgcgatg tggatgtgcg cacggcgggc cgcgtcatgc gggatcgcaa
    16261 catcggcagc ttgttcgtca ccaacggcaa agacgtgatc ggcatcatta ccgacacgga
    16321 tatggttcgg cgggttgtgg cgaccggggc cgatatgacc aaaacgacgg tggaacagat
    16381 catgtcggcg ccgctcgtga cgatcgaaga gcataagacg ttgttggacg cgaacgactt
    16441 gatggctcaa acgcatcttc ggcatctcgg ggtgacgaag gacggccaac tggtcggcat
    16501 gatttcggtt cgcgatttgg tggtcttcct gaccaatttg ccaaggaaat aagcgcatgg
    16561 cctttccgat gcagcgcatg cgtcgaacgc gggaaaccga gccgcttcgg cgaatggttc
    16621 gagagacgac cctgagcccg agtgacttca tctacccggt gtttgtcacc gaagggcagg
    16681 ggcggcgtga accgatcaat tccatgcccg gacagtgccg tgtctccatt gatctcctga
    16741 tcaaagaggc gctggaggtg aagtcgttag ggattcctgc cgtgatcctc tttggcattc
    16801 cggatcgaaa ggacgagcga gggagctcgg gattcgatcc gaatggcatc gtgcaacgtg
    16861 ccattcgcgc cctcaaagag caggttcctg agttggtggt cattaccgac gtctgtatcg
    16921 acgagtatac gagccacggc cattgcggga tcgtgcgcaa cgggaaaatc gtcaacgacg
    16981 aaacgctgga ttgtctgagg gctatggcga agacgcatgc cgaggcgggt gccgatatgg
    17041 tcgccccgtc agacatgatg gatggacgag tggccgccat tcgtgatgag ctggatcggg
    17101 ccggattcgt tgacattcca attatggctt acgccgcgaa attcgcctcc tgcttttatg
    17161 cgccgtttcg cgatgccgcc aactccagcc cgcagttcgg tgaccggcag tcttatcaaa
    17221 tggatcccgc caacaagcgg gaggcgttac gggagattga tctggatgtc gaagagggcg
    17281 ccgatatcat tatggtcaag ccagccatgc cttatctgga cgtcatcagt gccgcgcgtg
    17341 agcgaacgct cctgcccatc gccgcctatc aggtgagcgg cgaatacagc atgatcaagg
    17401 ccgccgcgca ggccggttgg ctggatgagc gtcgagccat gatggagtcc ctactgtcga
    17461 tcaagcgagc cggcgccgag atcatcctga cctattttgc caaagaagcg gccgcgctgc
    17521 ttcagtcacg gcacgcatga aaatttctcg cggtcttacc ccctccatcg ccaggcccta
    17581 ttcggtcgtg accatcggca atttcgatgg tcatcatcat ggccaccggg cgcttttgga
    17641 tcgggtggtg tcgacggcgc ggcgcgagtc cggcacagcg ctggtgctca cgtttgatcc
    17701 acatccggtc aagatcctcg ctcctcaggt caatctgatg ttcctcacca cgccggaaga
    17761 aaagctggcc cgcttcgaag ccgccggcat cgatgaagtg gtgtttctgg aatttacgac
    17821 cgcatttgcc gcattgagtc ccgcgcagtt cgcgaagcag gtcctgtgcg atggaattgg
    17881 cacgcgggag ttgttcgtcg gagaacattt tgctttcggg aaggggcggg caggtcgcat
    17941 tgcggatctg cttgaactcg gcgcacagct ggggtttcga gtgcatccga cgccgcccgt
    18001 gacgatcgac ggcgaggtgg tgagctcgac caagattcgc cagatgattc aagcgggaga
    18061 tctgcagaaa gccattcgtt tcctcgggag accctacggc attgaaggga gagtgattcc
    18121 cggcgctcat cgcgggaccg agctgggatg gcccacggcc aatcttcgcc tgcctgaggg
    18181 gcgggtcatt ccgccggatg gtgtgtatgc cgccagagtc ctgtggaacg gacgcgcctt
    18241 ggactcggtc gtctacatcg gcaccaggcc cacgtttggg gctggtgaac ggttactgga
    18301 ggtgtcgatt ctcgatgaac ggctggagct ctatggggaa acgattcgtg ttgagctgct
    18361 ggggtttgtg cgtgaggatc gtgtattcgc ctctgcagag gcgttgtcca gacagattga
    18421 gttggacgtg gatgcagccc gcatccagtt gcgcgaggcg gcgttggata ccgtctctcc
    18481 cctccattcc cagtcatgag tgatggcagg tccttccaca gcgacgcctc atccgcttca
    18541 taataaggtc atccgagccc tccgtacgcg cgcattactt caaccgggcc aatcagtctt
    18601 ggtggccgtt tccggcgggc cggattccgt ggccctgctc tctctcctcc acgaactcgc
    18661 accggcgtgg aatttttcgc tgacggtcgt ccattgcaac tacgggctgc ggggggccga
    18721 atcggacggg gatgcctcct tcgtcgctga gctctgccgg aggctgaaga ttccctgtgt
    18781 cgtcaaggcg ctccaaattc ctgcgcgtga gccgggtcgt tcgagttcat tgcaggcgcg
    18841 tgcccgagaa ctacgctatc agttgttccg tgacctggtc actgagctgg gtatcgagcg
    18901 tgtggcgctt gggcatacgg cggatgatca ggctgaaacc gtcttactgc ggatgctgcg
    18961 cggggccggt ttgcgtggtt tgggcggcat gccgcatcgc cgagggaatc tcttcgtgcg
    19021 cccgcttctg actgtgacgc ggcgtgagat cctgtcttat ctggaaacga aaagtttgtc
    19081 ttttcggacc gattccagca atgcgacatc cgtctatctt cgtaacagag tccggcatga
    19141 actgctcccc gtgatgcagt cactggctcc ggcgacggtc ccgttgctgg cgagacaagc
    19201 cgacattgtg cgcgaagacg accggctact ggaacgcctg gctgcccggc gtctggtccg
    19261 ggcgaccgtc aatcgagatt cacagacgct cgcccttgat cgggcggtcc ttctgaaaga
    19321 ggagccggcc ctgcagcgac gtatgctcag gcaggcgatg cgcagtctct cgccattggc
    19381 catcggggtg cgtggagatg tggttctctc attgttagcc tctttgtctg cggggcgctc
    19441 aggcggttcg tggacggtgg gatccctggt ggtgacatcg gaacagggcc tggtgcgaat
    19501 tgcggtgggg gatcccgctg gtccgaagac agaatcgagc tctcaatctg cctcgggtgt
    19561 cgctcttcag actgtcacgc tgtccagctt gccttgtgag gtgccatggc cgccaacggg
    19621 tgccttgatc cgcatctcgg tggtgacgcg tgagcggggg atggcgttgc tgaaaaagcc
    19681 ttcttcaaca atcgctattt ttgatgctga caggctgtta ttgccggtga cggttcggtc
    19741 ttggcggcag ggggattatt ttttccccgc cgggatggcc ggtcgacgaa agaaattgca
    19801 agattacttc acagatgcaa aactgggtcg gtctatgcga gaacacatcc cactgctgtt
    19861 gtctcgagaa cacatcgcct ggatcgtggg gcaacggatc gatgcccgat tcgcggccac
    19921 cgcttcgacg acccgattta tcgtcgctcg tgtgacgcat cccgtgcgcc ggaaaggagt
    19981 cttgtaggaa tggaacgcat ttttggtcgc cccattgtga cgcaggagca gatgcgaact
    20041 cgaatccgtg agctcggccg acagatcgcg tctgactatg ccggcaaaga gttggtgctg
    20101 gtaggggtgc tcaaaggagc gtatgccttt tatgccgacc tggcccgagc catccgtatt
    20161 ccgatgcggg tagaatttat cgtggtgacg agttatggcg ccggcaagcg aagctccggt
    20221 aaggtcaagt tgatctccga cctgacggaa ccgatcgtgg gcaaggatgt cctcttggtc
    20281 gaggacattg tcgattccgg tctcacggtg cagtacctaa tgaagactct gtctcgacgg
    20341 aaacctcggt cgttgaaagt ctgcacgctg ctcagcaagc cggagcggcg gcaagtcgag
    20401 gtggatctgg aatatgtcgg gtttaaaatt cccaataagt ttgtggtggg gtatggtctc
    20461 gattaccggc aggagtaccg caatcttccg tacctggcgg cgcttgatca aggcgaggaa
    20521 ggggagcagg agtccgcatg aaaggttgtt ccccctgcgg aacctatggt aacattctgc
    20581 aggcttagct aattttcggt ctgcctaata tcctcaaagg agaggtggat gaattcacgc
    20641 gtaaagaatt tattgttctg ggtcgtggtc ggcctgttca tgattttgtt gttcaatttg
    20701 tttagcgtgc cgacccatgc gccggaagat gaagtcatat tcagcgattt catggccaag
    20761 ctcgataagg gcgaagtcat gaaggtcacg atcaaagcca accacatcag cgcgattctt
    20821 aaagaccaaa gccggattcg gacctatacc gctgaatacc cggaattggt gaagcatttg
    20881 cgcgaaaaag atgtgcagat tgaagcgcgg ccgcctgatg aaagcccgtg gtacatcacc
    20941 ttcctggtga cgtggggccc cttcattctg ttcctcggac tgtggttctt cctcatgcgc
    21001 cagatgcaga tcggtggcaa caaggccttg tcgttcggga aaagccgcgc tcgcatgttg
    21061 accgaagagc gtaagaaggt gacgttctca gatgtggccg gcatcgagga agccaaggaa
    21121 gaagtactgg agattattga attcctgaaa gatccgcgaa agttccagaa gctcggcggg
    21181 cgcatcccga aaggcgtcct gatcgttggc cctccgggaa ccggaaagac cctcctggcc
    21241 aaggccattg ccggcgaagc gggggtgccc ttcttcagca tcagtggttc agacttcgtt
    21301 gaaatgttcg tcggggtcgg agcttcccgc gtacgggacc tgttcgaaca gggcaagaag
    21361 cacgcgcctt gtatcatctt catcgacgaa atcgatgcgg tcggtcgtct gcggggcgcg
    21421 ggccttggcg gaggacacga cgagcgtgag caaaccctga accaattgct ggtcgagatg
    21481 gacgggtttg acacgacgga aggcgtcatt ctcatcgctg ccacgaaccg tcctgacgtg
    21541 ctggatcccg ccttgttacg tcccggtcgt ttcgaccgtc aggtggtggt gaacagaccg
    21601 gacctgcgtg gccgatcgga aattctgaag gttcatacca agaaagttcc tctgtcgacg
    21661 gatgttgaac tggaaaaaat cgctcggggt acgccagggt tctccggggc cgacctcgaa
    21721 aatctagtca acgaagcggc gctgtgggcg gctcgtctga ataagaagga agtcgagctg
    21781 atcgacttcg aaatggcgaa ggataaggtc ctgatgggtg cggaacggaa gagcatggtg
    21841 ctcaccgatg aagaaaagcg cacaacagcc tatcatgaag caggccatgc gctcatggcg
    21901 aagctcttgc cgggaacgga tccggtgcat aaggtcacga ttattcctcg cggccgtgca
    21961 ctcggcgtga ccatgcagct tccaactgat gatcgtcaca attattccaa ggatttcctc
    22021 tacaacaatc tcgccattct catgggcggg cgggtggcgg aggagttagt gttgcatgat
    22081 gtgacgaccg gagcgggcaa cgatttggag cgcgcgacgg atctcgccag aaaaatggtc
    22141 tgcgaatggg gcatgagtga aaagctgggg ccgctgacct ttggacggaa ggaagaggaa
    22201 attttcctcg gtcgagagct tgggtccaaa cgggatttca gcgagcaggt cgccctggag
    22261 atcgaccatg aagtcaggcg cctcgtgacg gaaaactatg aacgtgcgaa gcgaatcctg
    22321 accgacaata tgaccagtct gaaggcactg gccgaggcgt tgctggaaaa agaagtgctg
    22381 gacgcattgg agatcgatca gatcctcatt cagggatcct cctcacagac ggttcctgcc
    22441 tagtttgtaa actgccggct tcacaggggc acccatgggt gttcctgtga agcatgttcc
    22501 tcatatgagt tccaggccaa gggttgggac atcctgctgt cacctcatcg gtgcatgatc
    22561 tcccatccct tatctcgtca gcttacatca ttccatcgtg tgccgttctg agagcgcctt
    22621 aggacttctc ttgaccctcg acactccatc tccattccgt cctgtggtta ttacggctgg
    22681 gtcccataac atcaccattc aatcgaatcc actcgtcatg ggtgtgctca atgtgacgcc
    22741 tgactcattt tccgatgggg gggcctactc cacagttgaa cgggcggtcg atcatgcgag
    22801 gcagatgcaa gctgacgggg ccgatatcat cgacatcggc gccgaatcct cccggccggg
    22861 agcggaccca gtcgaggaag gtgaggagtt gcgccgcttg atccccgtcg tggaagccgt
    22921 gcgggaggct gtatcccttc ccatttcagt cgatacgacg aaggcggctg tggctcgttt
    22981 ggctattcag gcaggggccc ttatcgtaaa tgatatcagc gccttgcagg gagatccctt
    23041 gatggctggc ctggtggctg agaccggggt cgctgtggtc ttgatgcata tgcagggcac
    23101 gccgcggacc atgcaacggt cgccacggta tgggaacgtg gtggaagagg tcaccgtatt
    23161 tttgcggcag cgggctcagg aggcgatcgc ccgcggtatc cgtccaactc aaatcatcct
    23221 tgacccgggc ttcggttttg gtaagctcca agagcataat cttcacatgc tcgcagagtt
    23281 cgatgcgttc acgaagttgg ggtacccggt gctggccggg gtatctcgta aacagtttat
    23341 cgggaatctg attcggcaac cggttcacga acgggaattt ggtaacgccg gggctgttgc
    23401 ggtagccgtt ctgaaaggcg ctcacattat tcgtgtgcat gacgttcgag cgatgaaaga
    23461 gacggccacg gtcgtgtcag ccatttcccg tcacgcgcgt tcttcttctg tagaggtatc
    23521 caatgcgtaa attgttcggc acagacggtg ttcgcggggt cgcgaacctg gagcccatga
    23581 ccagcgaaat cgccatgcaa ctcggccgtg ccgcggccca tatttttatg cgccgggcgg
    23641 gcaggcatca ggtcgtgatc ggaaaagaca cgcgcttgtc cgggtacatg ctggaatcgg
    23701 ccttgacctc gggcatctgc tcgatgggcg tcgacgtatt actcgtggga cctctgccga
    23761 cccccgccat tgcgtttctt actcgaagtt tacgcgcgga tgcgggcgtg gtgatttctg
    23821 cctcgcataa tccgtatcag gataacggaa tcaagttttt ctccaacgaa ggtttcaaac
    23881 tgcctgacga ggtggaagcg cggattgaac aattgattat ttccgatgag atcaagcatc
    23941 tgcggccgac tgccgatgcg atcggaaagg cgtaccggat cggtgatgcc gagggacgat
    24001 atatcgaatt cgtgaaacgg tcacttcccc gcgacctcga ttttcaagga attaagttag
    24061 tggtggattg cgccaacggc gcggcctaca aagtggcccc cgcggtattg cgggagttgg
    24121 gtgccgacat tgaagtcatc gccaatacgc ctgacgggat gaacatcaac gaccattgcg
    24181 gggcggtgca tcccgagcga ctgcaggagg ccgtgcgccg gaacggtgcg cacattggca
    24241 tagcgctgga cggtgacgca gatcgagcga tcttcgtctg cgagcagggc cacattgtgg
    24301 acggcgatca tgtcatggcc gcgttagggc tcgatttgca tggtcaaggc cggctcgctt
    24361 ctcagacggt cgtgggaacc gtgatgagca attttggatt ggaactggcc atgaagaagg
    24421 cgggcattca gttgatgcga acgcccgtgg gtgaccggta tctcatggag cggatgttgg
    24481 ccgatggcta taatttcggc ggcgagcaat ccggccactt tattttcttg gatcataaca
    24541 ccacaggcga cggcttgatt tcggccttgc agatcctctc cttgatgaaa cgcacgggga
    24601 aaccgctgtc tgaactcgcg aaggccatga ctgcggttcc gcagattttg ctgaacgtca
    24661 aagtgaaaca caagccggac ttaaaccaga ttccggatat tcagcaggcg attaagacgg
    24721 cggaagccac gttgaacggg agtggccgtg tgttagtgcg gtattccggc accgaagcgc
    24781 tgttgcggat catggtcgaa ggggaacggg atgcgaccat tcgtgaggtc gccgattacc
    24841 ttgcggacat tgttcgcgct cgtatcgggt agtaccggct ccttgcccga ggcgtcggat
    24901 gctgccgttc ctgacgatca tctttatcct cggaatcgct ctcggctcgt acctccagta
    24961 tttccccctc agcattgggc tgtttttctt cgcatcgact atcgccgctg tgcttgcaga
    25021 gcggcgagga ctactcaccg cagggcacag tagtctgagg ttggcctgcc tgttctgcgg
    25081 ctgtctgtat tggagcctgt tctcctggtc gacgtcgcat gtcccggtgc ccgaccgacc
    25141 tgctgctctt ccgtcaacca ttgacggaac gatcgttgaa tcggttcgtc atgcccctgg
    25201 gcgattgacc gcgctcatgc aggtcaccgc caccgatgat cccgacctcg ccacgccgtt
    25261 ctctctccgt ttgacctggc gcgaccccga ccgcgatctt caacgcggcc ttcacattcg
    25321 tctgcgaacc cgtgtgcatg cgccgtctgg aactttgaat ccgagaggat tcgactacgc
    25381 cgcctatctg gaagcgcagg gagtggatgc cgtgggatca gtatcggggg ccggtgccat
    25441 tgaagtgcta ggtgcagatg agggattgct ggcatatccg atttcgtcgc gcatcgatgc
    25501 atggcgcagt caggtacgga atgcggcaga cgcgctgcca cagccggctc gaggtctgtt
    25561 cctcagcttg accatcgggg agcagggctt cctcgcgccg gaagtacgcg aatggttcat
    25621 gacgaccggg acggtgcaca tcctttccat ctccggatcg catcttggac tgatcgcact
    25681 gctgtctttt gcgttggtga ggaaagcctg cctcctgctt ccttccatgc tgctcttggg
    25741 actttcacga tggttgattc ccagtaggct ggcagcattg ctcactctgg tgccggtcct
    25801 gggctatacc ctgttggcgg gtgcggagac ggccacgatt cgttcggcca tcatgatcat
    25861 agtggggctg tggacgatat ggttgggcac gccgcagtat cttcttcacg ccttggcggc
    25921 ggccgccggc ttgacgctgc tggtgcaccc tgccgcgctt tatgatattt cgtttcaact
    25981 atcctatgtg tccgtgtggg tactggcgct ggcactgcag cgacagcaat ccatcgacga
    26041 attacccctg ccacagaaaa gcctcgctgg tcgcaccttc tactggttac aggaatcaat
    26101 ccggctgacg gcctgggtga cattggcaac cttgccatta gtggcctgct acttcaatca
    26161 agtgtcctgg atggggatct tcgcaaatct gctcatggtg cccttcgtgg gattcatttt
    26221 gttgccgatc agtctgctgt ccgccgtctg ggtcatcgtt tcgcacagca gtacgttgcc
    26281 cggtgccgga ctcattgaac tttttgctca ggctgtgacg gttccgacgc agtggctggc
    26341 tggtctgccg ggagcggaat ggttcgtcgc cgcaccgacg gttcccttga tggcgctgtt
    26401 ctacctgcta ggctgggcca tgctgactga caggccggct catgcgcccc tgctggtgaa
    26461 gcgcgccatg gcgttcggca tggcctgtct catcctctgg tggctgtggt ctccgcgtcc
    26521 gttcacgcgg gaggggcagg tgcggatcac gtttctcgac gtagggcagg gagatagtgc
    26581 cgtgatcgaa ttgcccaagg gggaggtcgt gctcatcgat ggtggtgcca cgtatgagcg
    26641 ctttgacatg ggacgcagcg tcgtcgcgcc cttcctctgg aatcgcggta ttcgcaccat
    26701 tgaccatgtg atcgcgacgc atcctcagtt ggatcatgcc ggcggactag cctggatctt
    26761 ggcacatgtt caggtagaga acttctggac caatggtgtc acgcgaagcg aggaattctg
    26821 gcgcaagatc gagcggacgt tggcccgacg gcatctccag gcgcaggtcg ctgagcaggg
    26881 gagacgactg gtggacgacg cagaatgtga tattgccgtg ttgaatccac cgccgggccg
    26941 gaacgcatcc tctgcgggaa aacagcaatc gttgaataat ctgtcggtcg tgactgagct
    27001 cacctgtggc ggtcgacgaa tgttgttcac aggggacatc gagcgggaag ggttggcccg
    27061 tatggtgcaa tccgggcatc ttcgtcatat cgatttgctc aaagttccac accacggagc
    27121 gctgagctca ttggatcgtg aatggttgag cagtgttcag ccagatatcg ccgtggtatc
    27181 tgcggggcgc cgcaacccat atgggcatcc agcgggtgag gtcatcgcag cctacgagtc
    27241 ccttgagacg cagctgtggc gaacggatca ggacggcgcc gtgtgggctg atcttgacct
    27301 gaacaagcag tctctgacca tgcacagcac cagagaatgg cagcttcaac cagcgttaat
    27361 cccgatccct cagtgggcga ttgagcagga taattttcat cgcctgtggc gccggtggaa
    27421 ctggaactag gcacggtccc ggtctgagca tcgtgattct tatcgctccc gccgggcaga
    27481 aatccccagt tgcatggctc ccgaatgtgc cgggtgcccc gtttttcacc aatgcggctg
    27541 tcaattttgc cggtgtgggg aaaacatgtc ggcataagtt ggcaatattg cgaatggaac
    27601 cgggagtgtc atcaaggcat agcacatgca gagatgttca acgcctggtc acggcagatc
    27661 ttccgctgac ggtggggcat agctgacccc ctgcggacga taccgcagcc cccaggataa
    27721 agagcgagag aaggttaggc gatgatggcc aatcgactgt tgtttctagc cccggttcca
    27781 ccctccgatc accagggcgg tggtcctttg cggatgttgc acctgctacg ttttctgggg
    27841 agccgctttc acgtggacct ggtcgctccg gcggttgatg gctgggagaa tgcacagaac
    27901 tcattgcagg gcgtgtgcgc ggagacgacg tttgttccgc ctcaaggtcc cggattactg
    27961 gatcggctcg gacgggtcag cccctacgtg aaagatcgcg ccttggcttc tgtcgtgcgc
    28021 gagcggttgg ccagtggagt ctatggcgca gtccacgtcg aaaagcttgg actgattgca
    28081 tcgttgccga cgcaattggc aatcccggtg gtgttggata tttggtcata cggtctgcca
    28141 agcccgctag gaatattcaa gagcggaggt ggaacgaccg gtcggccgaa gccatttgtg
    28201 cgtcggctca aattggggtt gtacgaccga tggtgctggc cgttgacctc ttgtgtcacg
    28261 gttgtttcgg aggaagaccg gatacgttgt gagggcgcac acccaggcca gcgtgtcatc
    28321 gtcgtgccca acggtgtcga ttgtcaggcg attcgtccga aatccgatca tgccctgacg
    28381 tcacccgtgt tgctcttctc aggcgacatg ggcgctgagc cgaatgtgga ggccgctacg
    28441 gtcctcgcca cggaagtctt tcctgcgatt cgccgggaat ttcccaaggc ggaactccgg
    28501 ctggtgggga gaaacccgga tccgcgggta gctcgattgg ccggtccagg gatcatggtc
    28561 accggggcag ttcccgacat gcaggtccat ctcagggccg cgaccatcta tgttgcgccg
    28621 catttcaccg gccccgcgac gcgcacgaaa ttgttggaag ccatggcagc gggattgccg
    28681 atcatcacca cttcactcgg gactgaaggg atgaaagtgc agccgggccg tgatctgctc
    28741 gtggccgatc aaccggccga tatgatcgaa tccatccaga cgttgctcgc gagtcagccc
    28801 gatcgtgaac gttttgccca ggctgcccgc cacatggcag aggtctggta tgactggagc
    28861 cgctgtctct ggccgctgga gccgctctat caaaatctgc tcgccccgaa gcccgccgta
    28921 gcctgttgag ggtgtggtcg caaccagcta tgcctgccac gtcatcgcgc tcagtgcaac
    28981 ccccgcttca ggaatctgtt gtgttctgcc taccgtccac gtagaatttt gtgcccgtgc
    29041 acgtcgtagg ccctatgacg attgagcaaa gcctcccact gacaccatga cgttgatccc
    29101 gcactccaag ccctcgctgg gacaagaaga actacgagca gtgaccgagg tgcttcggtc
    29161 gggccagatc actgccgggc ccatggtcgc gcaatttgaa cgagggatcg ccgcctacat
    29221 cggcctgcat ggcggcgtgg ctgtcagttc cggcaccgtg gcgttggaat tggctttgcg
    29281 cgcgctcggc gtcggccatg gcgacaatgt catcctcccc agttatgtgt gctcggcacc
    29341 ctggctggct gtgcaacggg tcggcgccca agccagactc gtcgatatca atccgactac
    29401 ctataatctc gatcctcaga aagtgcgcaa agcccgcacc agccgtacgc gcgcggtaat
    29461 cgttccccat atgttcggcc tgccggcgga tttgaccagt ctgcagtcat tggacatccc
    29521 gttgatcgaa gattgcgcac aaacactcgg tgccatggag cagggacgcg cggtcggcag
    29581 cgttggaacc ctgacggttt gttcgtttta tgcgacgaag ttgctctgta ccggtgaagg
    29641 cgggatggtc ttgtcgaacg atgaagcttt gctcgagcga gttcgcgcgc tccgcgaata
    29701 tgataaggcg ccatccctga attcggcggc gggcaattgc aaattgacgg atctgcaagc
    29761 ggcgatcggc gtcgcgcaac tgaatcagtt gggagagttt ctcgacaagc ggtcggcctt
    29821 ggccgccttg tatcgtgaca tgctgtcgcc ggagttgttt acaatgcctg tcgtgcctgc
    29881 cggtcggacg cacgtctatt tccggttcgt ggtccgactg atgaacggat tacagtctgc
    29941 cgacgacttt acggactatc tttcccgcct ggcccaccgc ggattgcaat gccgcaagcc
    30001 ggtgtttcgt cccctgcatc gctatctcga cttgccggac tttcccgcca gcgatgaggc
    30061 cgatgcgtcg gccatttcgc tgccgattca tccctcactg acggaagaag cgatccggca
    30121 ggctgcgcac atcctgcagg aagagctgca ctgacgcagg cgtcccgccg aatcgttctc
    30181 ccttgaccct tgcctgagtg atcggcatga tgtgagcgaa tgttcaacgt gatgtgacgg
    30241 cgtcacattc ttccccagct gaacccggag acaatcggca tggtgcccac ggtcgagtgg
    30301 aaagagggag cggttcgcct tttggatcag agtcggcttc catcgcaggt cgaatttctt
    30361 gactgccgcg actatcgctc agtcgcgcag gccattcgag agttgaaagt ccgcggcgct
    30421 ccggcgatcg gtgtgacggc tgcgatgggc gtggccctcg gtgcgcagag tgtggacgcc
    30481 gacgagtatt cctcgttcgt caaacaggtc gacgcgattt gtgatcacct ggcggcctcg
    30541 cgcccgacgg cggtgaattt gttctgggcc atcgcccgca tgaaacaaaa gctggcatca
    30601 ctcaagggta tgccgatcgc agagatcaag cgtgagctga tccgtgaatc gcaaagcatt
    30661 ctcgacgagg acatcgcgct ctgcaaggcc atgggccgtc atggggcgac cgtgattcaa
    30721 aacggccaga cgatcctgac ccactgcaat gccggcgcat tggcgacggc ggggtacggc
    30781 accgcgctgg gtgtcatcag ggctgcgtgg gaacagggaa aacagattcg tgtgattgcc
    30841 gacgaaaccc gaccggtctt gcagggcgcg cgcttgacgg cctgggaact catgcaggac
    30901 aagattccgg tgacgctgat cacggacaat atggccggca gcctcatgcg gcaggggaat
    30961 attcaggtgt gtgtcgtcgg cgcggatcgg attgcggcca atggtgatgt cgccaacaag
    31021 atcggtacct actcggttgc agtgctggcc cgggcgcatg ggattccttt ttatgtggct
    31081 gcaccgtaca gcacgattga tctcgccacc aagaccggcg ccgagattcc catcgaagaa
    31141 cgaaaccccc tcgaagtgac gtctatccac ggcagccatc ctgtcgcgcc gtccggtgtc
    31201 gctgtctata atcccgcgtt tgacgtgact ccggccgaat tgatcaccgg catcatcaca
    31261 gaacggggca ttttcaagcc gggggaactc gccggtgcat tcgggacgtc acgccctccg
    31321 atcaagtccg gcaacgtttc ctgatactgg cttctcgggt aggtgtggca cattattact
    31381 catcctcgag tgccgaagaa tctcagagcc ctttatcttg ccagcacttc cgtcgcttct
    31441 ttataatgcc gcgagagtag gccgttgcga tatttttagc tgaaggagct gacgatccat
    31501 gagtgaacga acccttgcca tcatcaagcc ggacgccgtg aaaaaggatg ccatcggcga
    31561 cattattaat cgatatgaaa aggcggggtt gaagccggtc gctatgcgtc tgatgcacct
    31621 gtccaaagcc acggcgcaag ggttttatgc cgttcacaag gcacgcccgt tctttgatag
    31681 cctctgtacg ttcatgtcct cggggccctg tgtggtgctg gttcttcaag gcgacaacgc
    31741 ggtgaaaaag aaccgcgaac tgatgggggc gactgatccc gcaaaggcgg agaacgggac
    31801 gatccgggcg gcccatggcg cgaacatcga attcaatgcg gtgcacgggt ccgatgcgcc
    31861 ggagacggcg aagtttgaaa tcgggtattt ctttcctgaa atggagttgg tcgggtaggc
    31921 gcacattgag cgcaagcgtt tcccacgtca tgcagactcg acggccgttt tccggccgtc
    31981 gagcatccct tcccctggtg atcctgctga tggcgatcgg cagcggttgc gccgggccgc
    32041 ccgcagttgt tccggttgct caacccatcg aaaatccggt ggtcgaggcc gagaccaggc
    32101 tctggtacca agcttcgagt gccttttctg acggccgcta ttccgcggcg acgcatctct
    32161 acgaacggta tctgaccacc tatccgaagt cgcggcgcgc gaatgaagcc cactgggaac
    32221 tggcgcagtc gtatgaacag atgggggagg cggcgggcgc gctgaaagag tatcggatat
    32281 tggcagggcc ggagggtgct ccgctttcga cgcagagcgc ctatgctgag cgagccctgc
    32341 atcgtatcga agccttgcgg aatcagtcga gcctgcccgg cgccaggtct ggtcataccg
    32401 cactctacgt atcgttcagc aatttgcctc cgatggctca agtggaaacc tggattcagc
    32461 ggctgagcgc acagggcgtc agcgccattc ttatggatgc cagtttggag tcgtcgttta
    32521 ccaggccgcc tgcaccgacc ggtcctcctc ccagcagccc cttgccgaag ttgcccgcag
    32581 gcgcgttgtt ttcgacctcg caggggcctg tcatgacgga ttggttcagt gtgatggtgc
    32641 cgcaggcgca tgaactaggc atctcggtct ttgccgtgat tgaccttttt cacgcgccgc
    32701 tctccgatca ccggcaggaa tcccggacgc tgctgtatga cccgacgcgg cgaaaggtgc
    32761 actcctggac gcagttcgat cttctcgctc cgccggttca gcaggaagtg gcgcagttgt
    32821 tggccgactt gctgaggagc ggggttgacg gcatggtcat tcgcgcgcgc gcggagaaca
    32881 gctttgccta tgaggtcagt gacggcgtgt tgcgccagtt tgaggcgcag gtacgtcagc
    32941 cggcgtcgga agtggcccag gcgctccgtg agcggatgtc cgtgcggcag gacgcggggg
    33001 cgctcgcctc cgacaagacg ctttggcggt gggtcggctg gaaatcacgg caggagctcg
    33061 atgtgctggc gcgcctgaag aaatatttgc agaaggccgt gccgcggtta cgcatggtgc
    33121 tggagattca tcctgaagcc ttgtcgaatc cgacgtccgc cctggtgaac tatggagagg
    33181 acgccgccga ggcccgccgc cgaggttttg atttgttatt gggcggcccg tcccgtgagg
    33241 catcggatgt acgcacgttc ggcgggtcgt tcaagggttg gagtcgcgat gtaattcaat
    33301 ccgagaaagg cgagccgcaa actcttccac aaggctgggt cttgctgcga acgccaaccg
    33361 agcatggtgg tggcatgttt atgggattgg cccagcaggc ggacgaattg cggacgccgg
    33421 acatccgtca tctggtgttt gtgccggatg cgccgccagc gattccttga caaagattcc
    33481 ggcagctttt acgatccggc ggcacaaaaa tattgaaggg gcttcttatc aacgaggtcg
    33541 cgccgagcat ttccagtgga ctgagactgc tactgggagg ggtgtcagcg accgatgcaa
    33601 ggggagagtc atgataccgt ccaatctccg ttaccatcag gaacacgaat gggtccgcgc
    33661 cgaaggtcaa caggcgacgg tcgggatcag ccattttgca caggatgcgt tgggagatat
    33721 cgtgttcatc gatcttccaa aggtcggtgc caaggtgact gccggtcagc agatcggcga
    33781 ggtggagtcg accaagacca cgtccaccat ttacacgccc gtcagcggca ccattgcgaa
    33841 gatcaacacg gagctcaaag accatcccga ggccgtcaat gccgatccgt atggcaaggg
    33901 gtggatggct gtcattgatc tcacgactcc gggtgaagtg gacaagctca tgacggctgc
    33961 tcagtacgaa gagtttttga gcaagcagaa gcactaactc cgaacatcct tgcaccattg
    34021 aataggcgac gcatcctcgt atgcagcctg tgattcaatc ggcaccggtt tactgagtca
    34081 ttcttatgtc gaagcatctc gcaattcttg gcgccggtcc cggagggtat gtggcggcga
    34141 ttcgcgccgc gcagctcggg gcgcgtgtga ccgtggtcga aaaccaggcg ctcggcggcg
    34201 tgtgcctcaa ctggggctgc attccgagca aggccctgtt gtcggtcgtg gagctgggtg
    34261 acaaggccaa aaaggcgaag gacttcggcc ttctcctcaa cggtccggtc gcctatgacc
    34321 ctgccatcat ggtggcgagg aagaacaaag tcgtctccac gctggtcaag ggcattgcca
    34381 cgttgtttaa gacctggaac atcgaacatg tggagggcac gggtgagttg gtggatgccc
    34441 gaaccattcg ggtgaccaaa ccggatggga ctaccttgca agtggccgcc gatggaatca
    34501 tcgtcgccac cggctcctca tggccgaacc tgccgctctt tcccatcgac ggaactcaga
    34561 tcattaccag caagcaggcc ctggacctaa cccgaatccc tgccagcctc ctcatcgtcg
    34621 gcggtggcgt ggagggctgc gagtttgcct ctctgtatag cgggctggga gcccaggtga
    34681 cgctggtcga attggtcccg cgaatcctgc cgctggagga tgaagagatc agccagatga
    34741 tggagcggga gttgaagaag cggggagtgg acattcggac cggcgttacc gtcgatcaga
    34801 tcgtccggca gtccgacgtg gtgacggccc atcttcgtga tggcctctca ttgaacgtgg
    34861 agcaggtgct ggtgtcggta gggcgaggat tcaattcgcg cggaatcggg ctggagaaag
    34921 cgggagtgca ggtcggctct cgcggtgaaa ttgtcgtcaa tgaacgcatg gaaacgaatg
    34981 tccccggggt ctatgcgatc ggcgatgttg tgggtaaggc gatgttagcc catgtcgcct
    35041 cgcagcaagg caaggtggcg gtcgagaatt tcatgggtca cgcgcgcgca atcgattatg
    35101 acgtcatccc gacgggcatc tttacgttac ctgaaatcgg acgagtggga ttgacggagc
    35161 agcaggcccg tgatcgttgt gtgggcgccg gcaaggatcc ccaacagtcc gtaaaggtag
    35221 gacgtttccg ctacggcgga ttggggaagg cccaggcgac gggggatatt caaggattgg
    35281 taaaggtagt ggtggatacg gagagtgacc ggattctcgg tgtccacatt ctcggcgccc
    35341 atgcaaccga tctgattcac gaagccgcgc tggcgatgca tcttggcgcg accgtcgcgc
    35401 gtgcggcaga gatgattcat gcgcacccga ccctggccga gggactcatg gaagccatgg
    35461 aagatgtgca tgggcacgcg atccatctcg cgcgaaaacc ggcaacgaca tgatgcaatc
    35521 attgctaatc aaagttgcca tgctcgccgt gacgctgggt tcattggtgt ggattgcctc
    35581 ggtcactccc gtgacgaagg tgatgccggg acaacctcaa ctgctcccga aacattccgt
    35641 gacaacagtg tcatctccgc caccggtgga ggtgcctgcg acgaggccgg ccctggcaag
    35701 cgtcagtcag gcgggaacgc catcgcgtcg acaggtggcg atgccgattg aagagtccga
    35761 atctgcatct gcgcgtgtat ccgagaaagc gtcaacagac tcgccttctg ctcgacaggc
    35821 tggtgctcga caggtcgacc tgaaccaagc caccctctca gagttggagg cgttgcccgg
    35881 catcggaccg aagttagcgc aacgagtcat tgagcatcgc gatgagaatg gccccttccg
    35941 ttctgtagac gacttgcgcc aggtgaaagg catcggccgg aagaagttcg accgcctccg
    36001 tccacacgtc ctcgtcacca acactaaatc gttagcacga cacaaaggaa ccctgtgaca
    36061 ccgttagcgc agcagttgga tctcatactt cgagggatcg ttgaggttat tcagcagagc
    36121 gagctggaat caaagctggc tcgctcgatc aaggaaaacc gtccccttcg cgtcaaggcc
    36181 gggttcgacc caactgcgcc tgatcttcac ctgggccata ccgtgttgat tcataagctc
    36241 aagcatttcc aggacctcgg gcatcaggtg atcttcctca tcggcgattt taccggcatg
    36301 atcggtgatc cgaccgggcg atccgaaacc cgcgtcgcac tgtcgaagga aaaggtgctg
    36361 gagaatgcca agacgtatga gcgtcagatc ttcaaaatcc tcgatcccgc aaagacgcag
    36421 gtggagttca acagccgttg gatgagcacg atgaccgccg acgggttgat cgaactcagt
    36481 gctcattaca cagtcgcgcg catgttggaa cgggaagact ttcacaagcg gtatacggaa
    36541 ggcacgccga tcagcatcca tgagttcatg tatcccttga tccaaggcta cgattcggtg
    36601 gcgctgaaag cagatatcga attaggcgga acggatcaaa aattcaatct gttgatgggc
    36661 cgggatctcc agcgggacta cgggcaggag gcgcaggcgg tcatcaccat gccgttgctt
    36721 gaggggactg acggcgtacg caaaatgagt aaaagcttcg ggaactatat tgcgctggaa
    36781 gatcagccgg ctgatatgtt cggcaagctt atgtcgatca gcgatacgct catgcatcga
    36841 tactatgagc tactgaccac tgagaatttg gctggggtaa aggcggcgca tcctatggat
    36901 gcgaagcagg ggttggccga gcagatcgtt gcccggtatc acggcgctga cgccggccgt
    36961 gaagccagga ccgcgtttca gcagaaattt caggcgcggg agtttccgga acagccggac
    37021 gcgcatgtcg tcctcacgcc gtccgatgtg aaagatgcga gcgcaccctc catcgggctg
    37081 gtcgatctga tcgcgaagac cggactggtt cccagcaaga gcgaagctcg gcggttgatc
    37141 gtgcagggtg gggtggaaat taatgagcag aagatgaccg atgcgaatgg cgcggtccca
    37201 ttgctcgtcg gtcagttgct tcgtttgcgt gtcggtcgca gaaaattcgc cgtggccgag
    37261 tacaaggatt gagcaagtgg ctttcaggtt ccttgactta gcgtcgttgc cagcgtagga
    37321 ttcgacttct cttcaatcag gggcgggcgt agctcagtgg tagagtcctt gcttcccaag
    37381 cagttcttgt catcccctta atcgggcggc tgatctggtc aaatccgatc tggaagcggc
    37441 ttctcctcgt ttcttcctgt caaatttctt aagtggtatt aagctgtcgt caggtgagtt
    37501 ttgttgtttg gctgacactg taatgacacc aatctcattg gtagtcgacg gcgaaacagc
    37561 cacagcttgt tcctcaatga cgctgtttgg cctgctgcca gtcgagagga ctgaaggccg
    37621 ggtgagatcg ggcgattggg gaggaaagcc tagccgattt tctctggatt ccagagagcc
    37681 aacaaacata ggattgagcg agtgacactc gctctgatct tgtgcggctt ttctgccaga
    37741 gtcatttgtc atttcagggc caccgctaca aactgtttcg ggtcgagcga atcgtggctc
    37801 ttggccaaac tcgctatacg gcttgtttgg cgaagatcgt acccatgatc ccgtctgtga
    37861 ctttagcttt cgagagggaa ggatactgca ggagcagacg gcttacggtt gctgtgcgag
    37921 tggtggtttg tgtcttcctc ataagccttt tcacatggtc cttcaccgtg tattcactca
    37981 agtgtaggtg attggcaatt tccttgttcg tcaaccccgc cgcgaggtag cgggctacag
    38041 ctccttcgcg atgcgttaat cgtatttgct ctcgtgagga aaaggagtct gcttctcctt
    38101 gctcaatgat tggttcgata agcagaagca agcgaccaca ctcatggggg agctcatctg
    38161 gaactccaaa ccctcgtagt aatacaccac cttgcggtga agagaccatt tgctgcaccc
    38221 gcaccgcttg ccagtccata cctccgccgc ttctcgttag tgcaaccatc agttcatgac
    38281 acagacttga caactgagga ggaattagga ctcccgactg tggttctggc tgcgaaggga
    38341 aatgtaagga cagtaggcga tgcgcctcgc gactcagata gatcagctcg cctcgtagcc
    38401 taaagagtat gatcccggag atcatattgc tcattctcaa actccattgt gtctcaggaa
    38461 agctatcttc gacttgcgga cacttgagga gtctaaggtg gttaggggag ggcgacaatc
    38521 ccacatgagg tgcccctacc tctagggtag gaagacaaat ctgcgtgcac ctaaatacgt
    38581 gtaaatagga gagaatgctc ctggcaatca agactcaact gttctggatg tgagctgtcc
    38641 gaggccacgc catacaaacg gtgattgtat cccctgagcg tttcttagct ccgatggctg
    38701 gcgcagagtt ctcggcaatt cagattgtga ggacctaccg tgtaactact gttatccaat
    38761 aggcgtaccc ccatctgggg tgattgtggg gaccaggtgg caacccgtat gctccgccgc
    38821 gtgcgtgagt aacaacagaa atccaccacc gcgtcacaag gagcttcagc gatgcgttcc
    38881 acctatcagc cgattcgtcg actcctggtt gccttggctg ccctcctctt gcttgatccc
    38941 ggacacggcc tcgatgctaa ggagacggat acggagaccc caattggtgc agtgggtggg
    39001 agtattcatg gcgacccgtt tacaggcact gcaaccgcga gtattcccat agagatcccg
    39061 ccgggacgaa atggcgttca acctggcttg caactcgtgt atagccacag caatgggaac
    39121 ggttggattg ggatgggatg gaagctggag ctaggttcga tcgagcggca gagccgtttt
    39181 ggagtagact acgcgaagaa cgattacaca attcagatga gcggtatgtc gaccgagtta
    39241 gtacaagctc cgcttccagc ctccagcgat gagtatcgag cgaagtttga gggtagcttt
    39301 tccaaaatca agatgctctc gaacggaggc ttcgaggtca ccgacaaaaa agggacaaag
    39361 tacctattcg gccaatctgg tgtttctcgc gtgtctcacc ccaccgattc atcaaaaatc
    39421 tataagtggc ttctcgatcg tgtggaggat cgggacggca atttcatgac tgcaacgtac
    39481 agcgctgatc aggggcagga atatctgtcc caaataacct atacgggaaa tggagcagcc
    39541 cagccaggta ataccatcaa gttctatttg gagagcagac cggacggtca aactctctat
    39601 actgcgaatt tccaagttat tacagcacaa cgcctcaaga cgattgaggt attgtcaggc
    39661 acaaccagac tccgggccta caaactcgcg tactcaacga gcgccagtac aggccggtct
    39721 gtactgactt cagttcaaca gttcggcaaa gacgcggtgc tggacggttc ttttactgtg
    39781 accgggggaa catccctccc accagtgtca atgacttatg agaacgaaag tatgtcgctt
    39841 ggatccgatc agctgtgggg ctcgaagtcc tatggcatcg gctgtgccgc tgcatcgaat
    39901 tgccgcccca attttgccta tgtcgatatg aacgcggatg ggcgtacgga tttcatctat
    39961 caacgctccg atatcgacga aatgcacgtg ttgctttcta ctggttccgg attcggaagc
    40021 gatacgaact ggggaatgaa gtcttttggt attgcgtgtg ctggagcatc gagttgtcag
    40081 cctgcgtttt cgtatgccga catgaatggc gacggaaaga ctgattttgt ttatatgcga
    40141 tctgacctag accagttccg agttatgctt tcgactggaa ctgcatttag caccgatacc
    40201 gtatggggct ccaaatcctt tggaattgcg tgcgcgggag cttcgaattg taggcccaat
    40261 ttcgctttta ccgatgtgaa tggtgacggc aaggccgatt ttgtctatca gcgatccgac
    40321 attgatgagc tgcgagtact cctttcaacg ggaattggat ttagtgccga tacgaattgg
    40381 ggaatgaaga gttatgggat tgcctgtgct ggcgcgtcga attgccagcc gaattttgcc
    40441 ttcaccgacc tgaacggtga cggcaaggcc gatttcgttt atcagcgctc ggatatcgac
    40501 gagctgcgag tcatgctgtc gacggggacg gctttcgggc ctgatacaag ttggggtgtg
    40561 aaggcctacg gtattgcctg cgcgggggcg tccaattgta ttcccaattt cacatttgcg
    40621 gatatcaatg gcgacggcaa ggctgatttt gtctatcagc gatccgatat agatgaactt
    40681 cgggtgatgt tatccactgg gaccggcttc gcgactgata ctgcatgggg gatgaaagct
    40741 tacggcatcg catgcgctgg cgcctcaaac tgccgacctg cctttgggta tttcgatgtg
    40801 aatggggatg gaaaagcgga ctttgtgtat cagcgctccg acattgatga gcttcggatt
    40861 ctcctgtcca cgggcagtgc ttttaggccg gatgcagtat ggggaatgaa aggcttcggg
    40921 atcgcctgtg cgggcgcaat gaactgtcag ccgagcttta gttatgttga tacaaatggt
    40981 gacggaaaag gggagtttgc gtatcatcgc gatgcaacag accagattcg tgtattgcca
    41041 agttctcccg atggaatcct gttagccacc atgaccaatg gtacgggtgg ggctgctatc
    41101 acaacatacg caccatcaac tcaattcagt aacaccctcc taccatacac tacgcggctg
    41161 cttacctcca ctgtcacgtg cgacaactgg ggcgggagta gctgtgtggg actgtcctcg
    41221 acgacgacct atacgtatag cggcggctat caccatctcg cggaacgtga tttccgtggg
    41281 ttcaactacg ccaaggtgac ggggccggta ggaccgaccg gcgaacaact gatcaccgag
    41341 acgtggttcc atcagggcaa cgacgtggcg gttgatacca acaaccccaa cgtcgcgaac
    41401 gggtacacga agggcatgcc ctatcgtagc aaggtcaccg atgcgaccgg taaggtctat
    41461 agcgagacta ctactaccta tatcgccgac gtggatggcg ccgccccttt ctttactccc
    41521 gtggcgcaag tagacgcatc gatcgagaat ggtgccaagc aaacccggag cgtcttcgcc
    41581 caatacgacg cctacggcaa cgtgttgcgc gaggaccagt atggggatct ggccacgact
    41641 gccgatgatc ggaccgtggt gcggactttt gccaacaaca cgaccgactg gttgcttggg
    41701 tttccgacga gtgagactag ctatgcgggc atcggcacga ccacgcaagt ggcgcaaacc
    41761 aatctttatt acgacggcac gacgagctgt gccaccgcgt cggtgaatca agtgccgacc
    41821 aaggggcatg tgacccggac ggtacgctgg cttaacggcg gcaccagtcc cgaaaccagg
    41881 atggcctacg atgccttcgg caacctcacc tgtacccgtg acgccttggg tcaaaccagc
    41941 acggtgaact acgatagcac gaacaccttc gtcaagacgg ccaccaatcc actcggtcat
    42001 gtggtcacga cgcaatatta cggcgtggat ggtgtattgt tggacaaggg cctgtacggc
    42061 caggtcaaga gcttgaccga ttcgaatagt caaacgacga ccagcgaata tgacgctctc
    42121 ggtcgcaaga gtaagacgac cacaccggac ggcctaacta cgaccgtcat ctataactat
    42181 gggccgggtt tcacggtggg gacccagcac gtgctctcga gtacgagtgg tgccggcctc
    42241 gcgaccgcgc tgacgagcgc cagctatttt gatgggctgg gccgaggcgt caagaaggag
    42301 acgcctggtc cggacagcaa gacgattgtc acggaagtgc agtatgacag tcggggcgcc
    42361 gtgcggaaga agagcctgcc ctatttcaaa acgttagaat ctgtgacggg ccgctgggcg
    42421 acgacgagct atgacgccct tgggcgagtt gtgcgtatgg acagcccgga tggcacccgt
    42481 ggcctggcct gttttaccga ctgggtcacc gtcacgatcg atgccgccga tcatcgcaag
    42541 cgcgagacga aagacgcgta tggccggacc atccgggtgg atgaatatca gggcaccgcg
    42601 agtacctgtg acaccacggt cggcacgccc tacgccacca cgacctacca gtacgatgtg
    42661 cagggcaatc tgctctccgt gacggatgct cggggcaacg tctccaccat gacctacgac
    42721 acgttgaacc gtaagacggc catgcatgat cccgacatgg gcaactggag ctacgtctac
    42781 gatgccaatg gtaatctgac gaaacagacc gatgccaagg cccagatcct ctggttccgc
    42841 tacgatgccc tcaatcgccg ggtgcagaag gatttcacca cacaaaaggc gctgggcgcc
    42901 ggagatgttc gctacactta tgacgggacg acgaataacc ggaagggccg actgcaacag
    42961 gtggtcgatg cctctggcac cgtggtcttt cagtacgatg gcctcggacg catcagccag
    43021 accgacaaga ccttggacgg caccacctac accacccaga gtacctatga tggcttgggg
    43081 cggctcctga ccgtcaccta tccgagcaca ccttcgaaga ccatcagcta cgcctataac
    43141 gggccagtcc tggacaaggt attcgaaggc accacaacct atattcagta cacaaaccac
    43201 aatgcgctgg gccaggccgg tacgaccacc tacggcaatg gagtctcgac gacgacgacc
    43261 tatgccaatg ccaacaatac catctgtagc cagcagaact tccgcctgtg cacccttgtc
    43321 accaatggcc cagggagtgg cggtggcggg ggtggtacga gcacaacata cagtgccctc
    43381 gctgattttt ctggcacgca gggctcgcgt ggctggtatt acttgagttc gacgggaacc
    43441 cagcttacgt ggaacggctc ttattggact gggacggatg gctaccttgg cctctggaac
    43501 gatggcggtc atcccggcaa cagcacggat gcggtgcggc gttgggtcgc cccgtcagcg
    43561 ggcagcattc aaatcaccgg caataccttt gatggtgaca cctcttgcgg gactgatggg
    43621 gtggtggtca cgattaagaa gaacggggct gtcctttgga cccaaactat cgcggcgggc
    43681 aacacgacgg ggttcacctt taacctttca aatagcgtgg tcgttggcga tcagatcgac
    43741 tttgtgatca ataaactcac cacttcgtct tgcgacaata ccgttttcac tccgaccatc
    43801 gtgttgacga ctgccggggg cggaagcggg ccgttgctca atgggacgta tacagccccg
    43861 gcctcgaccg tgaatctttc tactgaaggc acggtcgatt ggacgcattg gggcctgagt
    43921 acagcgacaa gcttcaatca caagagtggc gttacttcac agatcagcaa tttttcggca
    43981 gtcggtggag gggtcgccgg gcagtacagt gacaacctcg tgggctatac ctggaccgga
    44041 ggtacgccaa cagcaagtgc caccaatagc acaaccgggc tttacatcgt gggagtgaat
    44101 aaaggcttcc aatttaccgt gccagccgat actacccaac ggacggtgaa agtctatgtc
    44161 agcgcttggg gtgcgcaggg caaaatggag gcgtttctca gtgacggtag tgccgcagga
    44221 tattcggaca cctcgctgag cgatccggga ctgagcagtt ctggtgcggc tcgggtctat
    44281 acgttcacgt atcgagccgc ctcagccaac caaaccttaa ctctgcgctg gaccaatttg
    44341 gtccaaaacg atccagctgg aggcaatgtt accttacaag ccgccaccct cgttacgacc
    44401 tcagggggag gaagtagcgg aaccgcctat caagacttgc gctatgtgta tggtttgaat
    44461 gggaatgtga acgatatcta tgacaacctg gtgccgggag gagccgggga tcaacacctg
    44521 agttatgacg ggttagatcg tctcacgttg gccagcgggc cctatgggac gaacggggcg
    44581 acggcctcgt tgacctgcac ctatgatgag ctgggcaacc tcacctttaa ctcacaggtg
    44641 ggcacctacg cctatcccgc gagcggctcg agtagtgttc ggccccatgc ggttacgacc
    44701 gccggcagta atacctacag ctacgacgcc aatggcaacc tgaccagcgg ggcgggccgg
    44761 tctttgacct acaacctcga aaataaaccg ctgacagtcg tgatcagcgg ccagactacc
    44821 acgttcgtct atgacggaga cggcgggcgg gtgaagaaaa tcgcgggaac caccaccacg
    44881 cgctatatca gcaagttcta tgagtgtgac aacacgagct gttcacggat ggtctttgcc
    44941 ggcggccagc gtattgccac catcggagcc agtggctcta tctactacta tcatacggac
    45001 catctgggct cctcaagcgt gatcaccgat agtgccggcg cgaaagccca agctgtcacc
    45061 tactttccgt atggcgcgac gcgtacgaac aattccacgg ctacaccggc catcgatgtg
    45121 ccctacaaat acacgggtaa ggagctcgat agcagcacga acctctacta ctacgaggcg
    45181 cggtactacg atcccacgtt ggggcggttc ttaagtgcgg acacgctggt accgaatccg
    45241 ctggaccccc agtcactcaa tcgatatagc tatgtgcata acaatccgtt caagtatacg
    45301 gatccgacgg gacacttcaa gatcaacgtc ggcaaatttt taaggcggtc attgggagat
    45361 ttgggaactg gaattgccgg agtcctggga caaacgttag gaaatgccct ctattggagc
    45421 ggtaatccaa tcctgggctc cttggttcaa ggcttaggca cagcaatgct tacacaatct
    45481 aagtctggtc gttatgttct ggcaggtgag ataattgtta cgacagttgt actgtattcg
    45541 gtttgtcctg gttgtgatgg cggtgccgca gctagcttca tcggaccgat gacggctgct
    45601 caggcgagtg cgggggcgac ggcggcgtct gtatgggggg gagcagcagc aggggccatt
    45661 ggaggggcag ctcttggagg ttatagcgct gccaagaatg gtggagatat cagcagtggg
    45721 gttctttttg gtgcaacaat tggggcagct tctggtgcct tgggaggagg gcttgcaccc
    45781 gctcctggca tgttcggtac aaatctccac gggtggttgg cgcaaacgat tgcgaagggg
    45841 ctcatcacta actttgggtc cggagcaatt agttcctacg ctggtggcaa agggaatatt
    45901 ggaaatattc tgatgggagc aacgatgaat gccgctctcg gaacggctac cacccccgcc
    45961 tcagacctgt tttgggggca gatggttccg gaagtcatgg gatatctccc cgccactgat
    46021 atggaaccgt actatatcaa taatgctgag ggagttcccg tacggggccc tgaaccatat
    46081 gccccattta atacggatct tgggaaaaga cttactggga tgatgggcga tgcaggcgcg
    46141 tctacattcg gtgaagtata cggagagaag ttgggagata ttattcatgg gcgacctatc
    46201 catataccct aggcaatgaa ggaatggagc tgacagttgt cgagtagtgc tacgtggtag
    46261 gttgtcaaag ccaggcggtc cgctgaggat agtcagatct cggacgcctt accttatttt
    46321 gattgggccg ttgagttggg tcctatgagg gacgggtgac gttggctgac gaactcgctc
    46381 ggcgtcaggt ggccgagtga gctgtgaggc cgacgctgag tgtagtccac gcgccaggct
    46441 tcgatgatgg cttctgcctc ggcgggcgac gcaaactgat gcacgttcaa gcactcatca
    46501 cggagccgcc cgttaaacga ttcaatgaag gcattttcca ccggtttgcc cggtcgaatg
    46561 aagtccagtt ggacgcctcg ccgataggcc caatcctcca atgcgcgcga ttggaattct
    46621 gtcccatgat ccaccgtgat cgagcggggc accgctggtg tgtccaggac acgatcacgc
    46681 acctgcccga ccatctctcc cgtcatccga aaccctacct ctcgtatagg actgtgccga
    46741 ctccagttat ccacgaccgt taagatccga aacgatcgcc cgtctgcgag ggtatcatgc
    46801 acaaagtcca tgctccagcg ctcggtcggg cccaccggga ccggagccgg gccgcggtgc
    46861 aacgcgatat gcttccgtct tcgcactcgc atccgcagct ggagcccatc gagccgatac
    46921 aagcggcgaa cgcgcttgcg attgatgagc cacccctcac gtcgcagcaa gacccaaatc
    46981 cgcatgtagc caaaccgagg ccgcgcatga gccaaatcac gaatgcgcag ccgaagcgcc
    47041 gactgatctt tcgcgcggct gcgtcgatac cacgaggctc gaccaaactg tgctcagcga
    47101 caggcccgcg cacagctcac ctgaaacgtc ccgtgaaacc actggggcca gttcccggcg
    47161 gcgggcgggc cgtagacttt ttttcgcaag gcctccgaga gcatgtgctt gtcgagggag
    47221 aggtcggcca ccaggcgttt cagccgacta ttctcctatt ccacctgccg cagtcggcgg
    47281 agttcgctca cgccgaggtg cgcgtacttc tttttccagg cataaaatgt ttgctcggcg
    47341 atgccgagct gccggcaaag gtcgccgacg ggggtaccgt tgtcagcttg gcggagggca
    47401 tagacgatct gctcttcgga gaatttcgac cgcttcatcg atccacacct ttcgtcgaag
    47461 accgttgaag ctggcatgtt ccccttaggt ttgatatgcc gtcgttttcc ggggagacgt
    47521 cacccacctc cggtttcctt catacacctc actcagtgtc tttgaagccg gcaggcggcc
    47581 acccagctag tagcctcgct ctggctaatc gcatgaacgt cttggggttt ctgtcttaat
    47641 ctcagaaaaa tagtgcccta acccactaac aggtcgagtt gcccttcttt cgagtcccta
    47701 ccactggggt gattgttgcc caggtctcct cagggtacac atgacagggt cttgctgaca
    47761 ctgattccgg ccatgcctgt tctcgagtgt gatttctgga tcgccggttt tttgttcagc
    47821 acaggacgat tttattggag acctgctgca tgtgtgaacc tgctgtgcaa ccatggtatg
    47881 gcatgacggc tctggctctt gtggtcatcc tgttgaccgg gtgcgcctca ccgaccttgc
    47941 gacgcgctga gcgcttggcc gaggacggcg aatgggataa agccgtggtg gcctatcggg
    48001 acgccctcaa gacggacccc ttcgatgagg ctctcaaaga caaactcgag gccgccaagc
    48061 aacaggcggc ggtcccccat tacaggaatg gacgtcgggc attggaggag cgtcgcatcg
    48121 tggacgccct gactgagttt aaggccgcgc ttgggcttga tccgacgaag atcgagcacc
    48181 atgcgggcat ggcggatgcg cttcgactga aggaagcgaa cgatcaactg cacacggccg
    48241 ataagctgct caacctgggc cgctccgagg aggccctcac ggcctatgaa cgggcggtcg
    48301 aactcgatcc gcatctgacg caagccctga cccacattac cgagatcacg gctcaacagc
    48361 gctccacgaa gatgtttggc ggatccagtc agcccatttc gctccggttc caaaacgcca
    48421 agctgaaaga ggtcttcgaa atcgtcgcgc ggacggcagg cctgaatgtg attttcgata
    48481 aggaagtgcg ggacgaaccg attacgatct tcctcaagga gatggcgttc gacgatgccc
    48541 tgaacctgat cctgaatacc aatggcatgg tcgcccaacg ggtggcgccc gagacgctgt
    48601 tgattatgcc gaacaacaag cagaagcagg cgcagtatca ggatctcctg atgcggacct
    48661 actacctcaa taacgccaag gcgaaagacg cggtgaattt gctcaggacg atgctcgaga
    48721 gcaagcatat ttatgtcgat gaaaaggtga atgcgatcgt cgtgcgtgag gaaccggtga
    48781 agctgcaact cgctgagaaa ttactctttg cgatcgatcg ccgtgaaccg gaggtggagc
    48841 tggacgtcga ggtgctggag gttaatcgca cgaagagtct gaagtacgga ctgaactatg
    48901 ccaaacaagc cggaacagga ctggtcccga cgggcggatc gggggggatc tccacggcga
    48961 caactcagtt tacgtatcag cagctcacct ccattggtcc acaaagttat ctgtttacct
    49021 tgcctgcgag cgtcatcctg gactttttca aacaggagtc cgatgcccag accctagcct
    49081 ctcccaaact ccgcgtgatg aataataagg ctgcatccat caacgtcggc gacaagcagc
    49141 cgatcttgct ctccacgacg aacgtgttgc cgggtcaagc tgcaacgggc gcggtgccga
    49201 cgacctccac ggtcacctcc attgagttca aggacgtggg cgtcaaattg acggtcgaac
    49261 cgaccatcaa tttgatgaac gaagtgatga tgaagatcaa ggtagaggtc acgcggttgg
    49321 gcgatcaagt gaccctgcag gccgcgccgg agatcaaaca gtttaaattc ggtacgcgca
    49381 cggcagaaac ggtcttgatg ttgaaggatg acgaaacggt cgtgctcgcg ggcctcattc
    49441 aggacgaaaa acgcaagacc aagtccacca tccccttcat cggcgacatt ccgattctcg
    49501 gcgacatcct gacctccaag caggatgatc gagtgtcgac ggaggtcgtg ttgacgataa
    49561 cgcctcgcgt ggttcggaac atggatctcc cgtcctccgc cgcacaggcc ttttggtctg
    49621 ggacggagaa taccttcgcg acctcgcagc tctatgctcc gcaagcgata cctgtctcgc
    49681 agtcatccgg gcagagccaa cggccgcatc ctgcccccag ttcattggga tcgaggcccg
    49741 gtacgcctac tgtcccaccg agtagcgggg cctcagcaac cgatacggga gtgcccactg
    49801 gagcaggatc ggcacaggag gcacttccgc catcaggtct tcctacagtg ccgaacgagc
    49861 agggcatagc tcctgtcact cctggctcag gtggagagct ggtcgcgcgg gcgaccggat
    49921 cgctggtgct tcggccattg gatgtctccg ccgcggtcgg gcaagagttt cgagtcgacc
    49981 tgacggcgtt gcacctcgaa cagatcagcg agacctcagt caccgtgacc tttgatccca
    50041 agctcttgga gttccatcga gtgacacccg gtatggtggc gctgg
//
LOCUS       DAOQDS010000029        49954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_219_length_49954_cov_21.826670, whole genome shotgun sequence.
ACCESSION   DAOQDS010000029 DAOQDS010000000
VERSION     DAOQDS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 49954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49954
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219_length_49954_cov_21.826670"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PK589_08935"
     CDS             complement(<1..302)
                     /locus_tag="PK589_08935"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNJ19765.1"
                     /translation="MMRTGLLIAVLVVSAIPAAAQLVKPDEPAVDLQSLHEVQEVVKN
                     IQSRYEKTKDLQASFTQKTRIEGFSTPVISTGHFYIKKPGKLRWDYIEPATEEIYV"
     gene            complement(299..2740)
                     /locus_tag="PK589_08940"
     CDS             complement(299..2740)
                     /locus_tag="PK589_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNJ19766.1"
                     /translation="MGVTTTAKRGDARSAPSRSSHVKREVIGVLLIAVGLLILLSLVS
                     FVPGDAKSIGASGAAGNQPKNMIGSVGALSAAACFFMVGGAAYLFPILLGLLGARCFT
                     PIPLTMRLRNAGSGLAAMVFLSALLHLEVTAVPTISSGWVNRGLAGGIIGQVLADGVR
                     SYFATTGAHIVVLTGLMVALLFTVPVSLTALLQRIPDWWIAARERMTGLMPEWPVKQE
                     EPMAPPKRARVKVARPAREMEEPELDREVQVVAEAVEQIAVPMIPPKIQPPMKVEKRA
                     TPEDEPAAAVATSPSVSDGYVLPDPQELLSDPSGPIARLSDDELKLQSEILTKALKSF
                     AIEGRVTEVRPGPVVTMYEFEPAPGTKVARIVNLADDLALALKAISLRIVAPLPGKSV
                     VGIEVPNPNREMVSMKEVVTSDAFSRSRSKLSLALGKDIFGGAVCADLKTMPHLLVAG
                     ATGAGKSVGLNTMLLSILFSARPDEVKLLLIDPKMLEFQSYDGIPHLLRPVITDPKSA
                     ARGLGWVVQEMERRYKLLAEAGVRSIDAYNRRISELHGAVSDVWQSGKAEQVELTFLS
                     EEERLSKGEDAEPAGDNGPTDSVKPSLPEPLPYIMVMIDELADLMMVAPKDVEDKIAR
                     LAQMARASGIHLVLATQRPSVDVLTGLIKANFPARIAFQVSSKTDSRTILDANGAEAL
                     LGRGDMLYLASGTGKLMRIHGSFVSDDDVRRVVEFVKKQALPSYCRELQSLKIEEAEE
                     EQAKDEVYEQAKDLVLSTGQASASLIQRRLRVGYPRAARMIEQMEAEGVVGAAGRDGR
                     REVLGRRGPVGGDEA"
     gene            complement(2839..3645)
                     /gene="rsmA"
                     /locus_tag="PK589_08945"
     CDS             complement(2839..3645)
                     /gene="rsmA"
                     /locus_tag="PK589_08945"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNJ19767.1"
                     /translation="MAASFPPALKRLGQNFLIDPNIIRKIVSLAALRPDDTVLEIGPG
                     RGALTAGLCAEAGRVIAVEIDPQLQPQLQETLSHCRNLDLRIGDALEFPFDSLPPRTV
                     VVSNLPYYVSTPILFALLDARAHLDRLVLMLQTEVALRLAAKPNSEDYGVLSVLTQEA
                     AEVEVAFRVSANCFRPRPTVGSAVVHLKLKTQDGVDPDRYERFRRLVRAAFAHRRKTL
                     VNSLRDEGYPVERISRAMQAVGVPLQARAETLTLDDYRALATAVDDDESA"
     gene            complement(3662..3982)
                     /locus_tag="PK589_08950"
     CDS             complement(3662..3982)
                     /locus_tag="PK589_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19768.1"
                     /translation="MSDAGSSAATIIDQQVKELDGIEKRTIQDFNTTLGTERVGQWKI
                     RTVALLQQHAGQAEADGLSRKKPGMSFTNDLIEEFSDEVESYRSYLVTLAKRLRAAVP
                     PNAG"
     gene            complement(4001..5044)
                     /gene="pdxA"
                     /locus_tag="PK589_08955"
     CDS             complement(4001..5044)
                     /gene="pdxA"
                     /locus_tag="PK589_08955"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNJ19769.1"
                     /translation="MARKRSQHSLRPVLGLTMGDPAGIGPEVIAKALADKALTRLCQP
                     VVIGSRPVMERTIKWLDLPLDVVTFDPASGARVKPGQVAVADPLERPLPKFRLGVASA
                     VTGAASIAFIREAVELAQAGSVGGIVTAPINKEAMNMAGFHYPGHTELLADLTKTKEF
                     GMMIVGGPLKIMFTTTHVAIHALSPLLTTQRIAKAIRLAHLGLTKYFGIARPKIGVAA
                     LNPHAGEHGLFGNEEQTSIAPAIKLARATGINASDPLPADTLFGKAARGDYDGVVAMY
                     HDQGLIPLKLVAFGTCVNLTVGLPIIRTSVDHGTAYDIAGKGVAEHGSLLEAVKVAAR
                     LAKSWSPVHSLSR"
     gene            complement(5034..6047)
                     /locus_tag="PK589_08960"
     CDS             complement(5034..6047)
                     /locus_tag="PK589_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19770.1"
                     /translation="MTVLLYVGMFVASVVITLAGCHLFTNGIEWLGKRLNISEGAVGS
                     VFAAVGTTLPETSIPIIAIFFGAGREQMEVGLGAILGAPFMLSTLVLPILALLLVLYA
                     KLGKRTATFKLNYGEVRVDISFFLVSYVLALTCAMIPSRVLHVAVAAVLLAMYVYYMK
                     LKFSAEDEESEGGDELEPLLFARRSAKPSYLVIGAQGIVGLLGLVGGAHLFVTAANSI
                     SAQLQVSPLILALLIAPLATELPEMSNSFLWLYRKKDRLAVGNVTGAMVFQGSIPVSV
                     GLLGTEWTLGTTALATMVLAVVAMSMSLLQALWSGHWRPWLLSGSALLYLGYTLFLYT
                     HGS"
     gene            complement(6106..7314)
                     /gene="larC"
                     /locus_tag="PK589_08965"
     CDS             complement(6106..7314)
                     /gene="larC"
                     /locus_tag="PK589_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNJ19771.1"
                     /translation="MARHLHFDCFSGISGDMTLGALVDAGLPFKDLVRGLASLKIDGY
                     RLTRKRVERGAITATKVDVLIDKGFRAPLTLSQIAKILRRSGLPPAVKERSQAVFDVL
                     ADAEGKAHGVEPSHVHFHEVGVIDSFVDVVGGILGLHLLDIRRVTASAINVGSGMLQS
                     AHGALPVPGPAVAALAVGVPIYAKGPERELTTPTGMALLKTVTTDFGCLPSMQVRQVG
                     YGAGTADPAQWANVLRVFIGDEASESGAMETIVELETNLDDVNPQVYDTVFDRVFAAG
                     AVDATLAPVIMKKGRPGNVLSVLAPREHTEAVLAVLFAETTALGVRIREVQRRVLPRQ
                     FASVRVNGQDVRIKLADIRPGLSKAAPEYDDCKRVAERSGRPVKDILDEALMVYRQTL
                     GTRPRRKGRA"
     gene            7486..8055
                     /locus_tag="PK589_08970"
     CDS             7486..8055
                     /locus_tag="PK589_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19772.1"
                     /translation="MTNSTPAPRVSLKRASTGRTFGLLLLLSGFCLLGDPSLLLAKTK
                     GKSSVPRPEPDLKILSLQAAPMPYRPQEGPFHFIATVQLPKEVDEHLMLEVSALVTSS
                     SMTSLRFLSIRQPVNEHVQTTPSANGDTPQKHVQVDLEWDGLDHNKQQIPIGSYEYEV
                     RVKLLSNGEKGQRTQMLSWPKRGKIVVKH"
     gene            complement(8089..8874)
                     /gene="larB"
                     /locus_tag="PK589_08975"
     CDS             complement(8089..8874)
                     /gene="larB"
                     /locus_tag="PK589_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNJ19773.1"
                     /translation="MNQDQLRALLMEVQQGAVAVPDALHRLRTLPYENLGFASLDHHR
                     ALRQGFPEVIFCEGKTVAQVVAIAKTLLLKNNSLLATRVEPAVARALLRMSKRATYHE
                     AARVVAIVPPKLVRRGSVLIVTAGTADIPVAEEARVTADIMGSKTDTLYDVGVAGLHR
                     LLGQQDRLHDARAIIVAAGMDGVLPSVVGGLVRQPVIAVPTSRGYGAHFGGLAALLTM
                     LNSCAAGVGVMNIDNGFGAGCLAHRINMVSEMDSPKPERAKRQ"
     gene            complement(8871..9896)
                     /locus_tag="PK589_08980"
     CDS             complement(8871..9896)
                     /locus_tag="PK589_08980"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNJ19774.1"
                     /translation="MTRSSSQRVAVIGAGAWGTALARHLAEQQISVCVWAHEPEVVQA
                     IDQRRENVVFLPGVRLPATLTATSHLSDALIGADCVIFAVPSHVARLVLSRLGPLLPQ
                     SIPFVSATKGIEETTLELMTQVMQDVLPDHMRHSLMVLSGPSFASEVSQGKPTALCLA
                     GQDAGAVKAIQALFMTPTFRVYADDDLIGVQLGGSLKNVMALAAGVVDGLELGHNARA
                     ALITRGLAEMIRLGMAMGADPRTFYGLSGVGDLILTCTGPLSRNHSVGVRLGKGERLE
                     GILASMQAVAEGVRTAKAALGLAQRCGVDMPIVQEVNAVLFADKPCRQAVGDLMEREA
                     KEEKALS"
     gene            10018..10377
                     /locus_tag="PK589_08985"
     CDS             10018..10377
                     /locus_tag="PK589_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ19775.1"
                     /translation="MKPCPACGRALPEINRYCTYCGTGVASDTAQKAMPQASEATRDQ
                     LNLHILYGMVTVLVVSVIMPPWETPPSQPAAFLGFHFILSPPQPDAIVSRLLLTIELT
                     TTAIAGLYLSFLFRSKG"
     gene            complement(10411..11211)
                     /locus_tag="PK589_08990"
     CDS             complement(10411..11211)
                     /locus_tag="PK589_08990"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HNJ19776.1"
                     /translation="MSRLLVCPPEYFQIDYEINPWMRRENAVVPSRAVGQWQTLMHVL
                     EQDVGVGVERMQPVPGLPDLVFTANAGVVASRRVVISRFRYPERRREEVYFAQWFREH
                     GYEVLTLEESLYFEGAGDLLGFPDTWFGGYRQRSDIRSFPKLSEIFQREIIPLELIDG
                     RFYHLDTCFCPLSGGELLYFPSAFDAYGLAAIAQRIPEEQRLVVPEDEAIRFACNAVC
                     VGKRIVIPAGCPRTMRLLESRGYRTHDVELDEFMKSGGAAKCLTLALD"
     gene            complement(11208..12452)
                     /locus_tag="PK589_08995"
     CDS             complement(11208..12452)
                     /locus_tag="PK589_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HNJ19777.1"
                     /translation="MTALQETVFLQGHIIDSLILAKVLDTILMMGGTFDLQDVNIGAT
                     RDAPSQARIVVRAHSSRLLAEILQAIQPHGAAVERDTECRFEPAPAAGIFPDEFYATT
                     HLPTQIRLDGEWVEVEGMEMDLGICVDPAKARARTLPMGSVKAGDLIVTGREGIRVTP
                     LARPRERDVFGFMESVVSSERPHQPVIADIAQRMLKLRAGYRQGQTGTKVLLAGGPAI
                     VHAGGREALTWLIEAGFIHVLFCGNALAAHDMEAALYGTSLGYGLTAGRSVPHGHEHH
                     LRTINRIRAIGSIHEAVRSGVITEGIMAACVRQDVNMVMAGTIRDDGPLPGVVTDSMQ
                     AQGAMRAEVQGVGLALLVASTLHAIATGNLLPASVPTVCVDVNPSVPTKLADRGSFQA
                     VGLVMDAASFLKELARELGWRL"
     gene            complement(12553..13605)
                     /locus_tag="PK589_09000"
     CDS             complement(12553..13605)
                     /locus_tag="PK589_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HNJ19778.1"
                     /translation="MPTPSWLDDTVPYLTASVPALGGRIRSTPEDFCVDERPLYLPCG
                     EGEHLYIKITKRGLSTPDLVLRLSSHLHVKAQTIGVAGLKDAQAVTTQMLSLQGVKAE
                     AVAALPTDERLLSVEVLGRHRNRLRKGHHAGNQFRLVVRDVREGSEELLGRLFEELVR
                     RGVPNYFGPQRQGRSGTNFQLGAELLQDAARRNKMPRAKRIWFMNAYQSYVFNHIVAK
                     RIDSIDRVLLGDWAMKSDNGACFPVEQPEVEQLRADRFEISPTGPLFGSRAPWATGEP
                     GEIERAVIAELGTTPEVLSKAGAECGFRGERRALRVRLNDLVWSLEGSVLTLSFWLPP
                     GSYATSVLREIVKTNA"
     gene            13729..14391
                     /locus_tag="PK589_09005"
     CDS             13729..14391
                     /locus_tag="PK589_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19779.1"
                     /translation="MRRRVVHTSCAIMFFFCACTVSQARSATAAARPIGIDVFAQLTQ
                     HIKRQADTDKEAFAIASQCMNFFYKQQRHKPAPPAVQQIAWIPAESSELAAADSDAQE
                     ADCRKRYPEGLDAVRTDFQRTQALLSLSLTFYEFALVGDGDDNSLYNARELHDILVAL
                     NLSAEPSLGAAAHLRSLTAHFDSLHEARGMEALMAGMGKLYDQGYRVTTADKAHLNRV
                     ME"
     gene            14388..15221
                     /locus_tag="PK589_09010"
     CDS             14388..15221
                     /locus_tag="PK589_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNJ19780.1"
                     /translation="MISTVMRWQELARVWIGHWLSRPFHHLFNLIPGVEIGLSATVVT
                     RLPLTHAPLAGRRAVHLSDLHLDRYRPRHRALIRAVADLKPDWIFITGDLVNVRDGLP
                     HLLRFLTELRRLAPVYVTLGNHDHYSGIRVDEFAEQFDRRKVTLLLNQVTFIRVATGE
                     LAIVGLDDPSLHRADLRCMPSAQPGRFTLVLAHAPNILDQLRPEHHTDLTLCGHSHAG
                     QWQIPGIPTFWLPPGCHGRTHGMYEKGGHRLYVNRGLGWSVLPMRVNCTPEIVLLEWA
                     A"
     gene            complement(15257..15901)
                     /locus_tag="PK589_09015"
     CDS             complement(15257..15901)
                     /locus_tag="PK589_09015"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ19781.1"
                     /translation="MSPAMPRHSQSGQANLTAVFLLLGLVVSGVWVWKRLSPEMQDLI
                     IDQALPLAALVLAVLVFVWMLVGKIRARRTRNQQRARLIKLFESQTAADKRLQLAFAV
                     IEVNEYRRVGLEAIAPRFQELFLTTLKRALGDKQHQVRGMAASHLGVIGDETVVPHLL
                     KALEDDHAYVRSSAALGLGRLRATAAKDKLAYVSKEDWDQTVRSRAREALERIR"
     gene            15989..16447
                     /locus_tag="PK589_09020"
     CDS             15989..16447
                     /locus_tag="PK589_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNJ19782.1"
                     /translation="MDLPDLKDDPHLKVLRPLVETYLAFWRADSRHVRSLRLTPSQFD
                     VIATLGDTKGLTCAELSTATLVTKGTLTGVLDRLEAKGLIRRTPVADDRRSTRICLTA
                     KGNKLFQTTFAAHIAFLRPFFQRALSAADADQLRTLLLRLHRSFQEKPVA"
     gene            16444..17202
                     /locus_tag="PK589_09025"
     CDS             16444..17202
                     /locus_tag="PK589_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ19783.1"
                     /translation="MIRLTVLGSGTNVHPTRAAAGYLLQTDQTFLLDFGPRTLSNLIK
                     SGVDRHSITHILFSHFHADHFADFIPFFFDAVIFCKYQGGTRPPLTLIGPHGTATLMR
                     SMMTTFPSFDRPPFRVTIREVADRSIRIGETRITPATVTHAPKLHCVGYRVEYQGHAV
                     AYSGDSQYCESLVRLCHEADFAVLDCSFPENRPGAGHLHAGLCGRVAQEAGVNQLVLS
                     HFYPIAEQYDVRAQAKHYFPGRVRIARDLLKLRS"
     gene            complement(17313..18173)
                     /locus_tag="PK589_09030"
     CDS             complement(17313..18173)
                     /locus_tag="PK589_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNJ19784.1"
                     /translation="MFVNESQIRALIRLLGDEDERIVKTISGKLIDFGDCAVPLLQEA
                     EIEQPEMADRIVTVLEEIRGARLEEELRDLIAFPDDQVDLETGAFILARYAYPTLDVQ
                     AYRRQLDRMAQDVREQIGSRVSGEETVRALNRYLFTEAGFKGNTKNYYEVENSYLNCV
                     MDRRTGIPISLSTVYLLIGRRLQLPVHGIGMPGHFLVKFDSDRYKIFIDCFNGGALLT
                     EKNCARFLTEAGYGFEDRFLQKSPTRAILARMIKNLLAIYNKLDEPLKKHRLPRFVEI
                     LGCEQREEGL"
     gene            complement(18200..18979)
                     /locus_tag="PK589_09035"
     CDS             complement(18200..18979)
                     /locus_tag="PK589_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ19785.1"
                     /translation="MKEYWQEIRALTMRWVRRLSREKFSMLFTLVQPMLFWLIFFGNL
                     FQRAADLQVTQSSSYISFLTAGVVVMTVLNNGLAGGVDLLFDKENGFLERLMSTPIHR
                     TSVILSRFIFVTTITSLQVLVILAVAWLFGVQPVTGLAGVATILLIGMMFGVGLTAIS
                     MAMAFSVKSHGDFFSVLGFLSLPMIFLSSALVPLAAMPGWMGFLAQFNPMTWAIDAVR
                     PLILQGWAEALPHVGMVIGVMVLFDALCLYGGAKAFRRAIG"
     gene            complement(19009..19980)
                     /locus_tag="PK589_09040"
     CDS             complement(19009..19980)
                     /locus_tag="PK589_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ19786.1"
                     /translation="MSRPVAIDVSHLGKTYDKVRAVEDLSFQVYTGEIFGLLGPNGAG
                     KSTTLRILITLLNPTSGSAKIFGLDSVKDADRVRQTIGYVPQERAIDRFLTGREHLEL
                     LADLYHLSQEDAAGRIPQLLKLVELEQQADRPAKTYSGGMKRKLDIACGLLPDPKILF
                     LDEPTLGLDVQSRLRIWDHVRAMRERGITVVMTTNYLDEADQLCDRIAIIDGGRIKAL
                     GAPNELKVGLGGDLVSLTVREHDRVEKLAAAIRDLPAIRAVTVTANGLDIRVDSPEKA
                     LPAILEAANRLTCQLEFIDYHRPRLDDVFIAHTGRSIKDDHPKAEDA"
     gene            complement(19980..20669)
                     /locus_tag="PK589_09045"
     CDS             complement(19980..20669)
                     /locus_tag="PK589_09045"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNJ19787.1"
                     /translation="MFPEPEREPSNRELSGKVQPFPVPERIPLFPLPNVVFFPKTYLP
                     LHVFEPRYRQMVADAAAAGQCIGMALLKEGWEEQYEGNPPIFSIGCVGRLASVQMLPD
                     GRSNILLQGLERYEIHEEFFEKSYREARVTLKPRDGSLSLDQALRRYLMGVLDEYLKA
                     DEEASPLHSLIRPDVSDEVFVNSLSTYLDCTPLEKQFLLEAEHVPQQARRLSDLIQFK
                     LAERRGAGGWG"
     gene            20849..21985
                     /locus_tag="PK589_09050"
     CDS             20849..21985
                     /locus_tag="PK589_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HNJ19788.1"
                     /translation="MTPLRLGCTIRLLLLAVLPVTAQAVATNETVHAFDMATPGYQYR
                     FPHDHGAHERFRTEWWYYTGHVVAADGHRFGFELTFFRRGMPPDQVQTLPSRWSIQQL
                     YLAHLALTDLDAGRFYYADKVSREGLGKAGADTDRLHVWVDRWSLLTNDPAASRHQLQ
                     AATDSVAINLTVIPKKPPVIHGHDGLSRKGADPGQASHYYSLTHLATEGQIRVGTETF
                     AVTGLSWMDHEFGSADLGLNVVGWDWFSLQLSDSTELMWYSLRRADGSSDPVSSGTLV
                     LADGRAHALDAQDLNIEPLAHWTSPRSNGRYPQRWRLTSATMGLDVTVESLLADQELD
                     TARSTGVTYWEGAVSVSGQVRGAPVTGRGYVEMTGYAERFTPKL"
     gene            22059..23108
                     /locus_tag="PK589_09055"
     CDS             22059..23108
                     /locus_tag="PK589_09055"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19789.1"
                     /translation="MSQRIRIATLLRRSICLLSFLLLACGSRESAVVSIALHPSNPNI
                     VYVATNDAVHKTRDGGATWEQFPAFTARRVTTLAIDPKLPATVYAGTMGDAIYKSPDG
                     GQHWLPHNVGLKEHVSFINEIIFHPDNTELIYLATTVGAFISKNGGREWEERMAGMKE
                     VHIVTCITMDHQHPRVLYAGTTGGTYRSEDGGTSWQKKNTGLIPEDVLNAAMALGVNM
                     LAVDPRNSEVVYAATTKGLFRSANRGESWEQIGQGLSNQFISTLALHPMDANIFYIGG
                     PDGVRKTTDGGKTWQSQNEGLTTTNIRTISLSPSDPQLLYLGTNGSGLYRSTDGGQHW
                     APIPLTGPQQPPPAS"
     gene            23129..23737
                     /locus_tag="PK589_09060"
     CDS             23129..23737
                     /locus_tag="PK589_09060"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNJ19790.1"
                     /translation="MPTLLLIRHGETDWNRSGQVMGNQPIPLNETGEQQALACAEMLA
                     QTPIAGIVTSPVLRAVQTAHIVGRRHAAPVHHAPGLREIGVGNWINRYWKDLADDPAK
                     RDWYLHPDRARPSGGETLCEVQQRAVAAVEQALQVAQDGPYVFVSHGDVIRAVLAHYL
                     RLDLNALRQARIDHAGVSGIEVTEAATHLLFLNHRPGLPRLA"
     gene            complement(23754..24233)
                     /locus_tag="PK589_09065"
     CDS             complement(23754..24233)
                     /locus_tag="PK589_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19791.1"
                     /translation="MSVRSLCLLVGVLLTGLTGCSHHHGSYEHAKGDYYWGKASQDVS
                     GLVEKHVKNPETVKQVNVVMGEMIAEIKSAREQHRQFHRALYELNANYSAAPEEFTKI
                     LDELNNSRMRSSAKILGLRFKMKALLTAEEWTALSEELKRYGSRYYGKGAEGASAHQ"
     gene            24341..24697
                     /locus_tag="PK589_09070"
     CDS             24341..24697
                     /locus_tag="PK589_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19792.1"
                     /translation="MGEQKAPQRGTGPVESLRDEFRQHLDVFYSRLKLAAPYDSVEKA
                     LGTLTTTLKALPPEELQRLGADQTQRWAHFRQAFVDSGLIQKHRGIIAGLARSSASPD
                     LPPEFNHLLDLYRPKT"
     gene            complement(24746..25375)
                     /locus_tag="PK589_09075"
     CDS             complement(24746..25375)
                     /locus_tag="PK589_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19793.1"
                     /translation="MMLAGAFAIVLFIGLIVGDWMYFTRLSSDATRYGYGIGQVQDRF
                     AALTVPKLLRAFDTNGFLSLPHGVARVFPDANRMIIRPTHRLFSMRFRTAWPLKGSIE
                     LASDSDGLRMVCTKRVPWSSSILTFLWFSLVGFGTLGFLITYVLQGGLESLGSVLLGI
                     GVTGVGLIVVAFGLLTVTVAYRIENTRLMLVYTELRDIALANEGAASRP"
     gene            25632..26318
                     /locus_tag="PK589_09080"
     CDS             25632..26318
                     /locus_tag="PK589_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ19794.1"
                     /translation="MDIAKVERVYTSYSGVYDHIFGKIFHESREACVRNLRIRPEENI
                     LEVGVGTGIALEYYPKNCNITGIDLSSGMLAKARQRQSHYHLDHVRLMLMDAGKMEFP
                     DDSFDTVMAAYVVTAVPDYRKVVNEMIRVCKPGGRIIMLNHFSNGNKVIAAVEKVISP
                     LCKHIGFRTDLSLNTVLEGTDLHVARKEKVNPMKFWHLVECVNRKGTKSSNGNGSVNG
                     NGNGRHGHVS"
     gene            complement(26379..27152)
                     /locus_tag="PK589_09085"
     CDS             complement(26379..27152)
                     /locus_tag="PK589_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19795.1"
                     /translation="MTHSIKTADDVRWLLTHTQGFRGGRVMDVHLAKRWMLDEESGRD
                     VSAGRVVTVTVRYHLQGVLRIAKLSLHGVSDFSVFEQDGGDCAALGMIQVELHEGLLR
                     FWFDPQGNLYVVCEEALLEEVSVPHSDGDIHQGAAPWIFQADSGDPPTVDWLLTQLDQ
                     VGVPCIWKSTPRRAGHHGTLCWEGELVSTADVNAGPTAALTVRAYEPIDGAGFGIRLQ
                     PRLATGRFSGRLLRAVTEVVTQRYVGTCLTGERGVSSEW"
     gene            complement(27281..27484)
                     /locus_tag="PK589_09090"
     CDS             complement(27281..27484)
                     /locus_tag="PK589_09090"
                     /inference="COORDINATES: protein motif:HMM:NF016397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19796.1"
                     /translation="MGSAFEDGQQAQLELAGLQRTLKWTNIQREQLLDRLDLLRLDNQ
                     RLQERVEELERQLAEAKQQQVLF"
     gene            complement(27633..28931)
                     /locus_tag="PK589_09095"
     CDS             complement(27633..28931)
                     /locus_tag="PK589_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ19797.1"
                     /translation="MSTVAIRAEGLSKHYRLGAAVQHNTLRDQLMHRLKSLTRWGSGR
                     SEPDPSFWAVKDVSFEVKQGEVLGIIGHNGAGKSTLLKILSRITQPTKGTVDIYGRVS
                     SLLEVGTGFHSELTGRENIFLNAAMLGMRRDEVRRKFDEIVAFSGVEDFIDTPVKRYS
                     SGMYVRLAFAVAAHLEPEILIVDEVLAVGDASFQQKCLGKMEEVSRSGRTVLIVSHNM
                     GIIEGLCERAILLENGRIARIGKTQTVVEGYADAIRSQASMSIGDRPDRVGLGEILLT
                     EIDLLDKDRHSVTSAITGRDVIIRMHYRCAEGKEFRNCRVSVSVNGRKGQDLFVLSTD
                     IVDPTPLTLRGTGYVDFILPDLPLTGGAYFLQSYIESNGHAQDWIKNVVPFPVLDADY
                     YGTGNLCPPGWEANGVLVRYRWESSDDLEGSTLPLRAREK"
     gene            complement(28932..29789)
                     /locus_tag="PK589_09100"
     CDS             complement(28932..29789)
                     /locus_tag="PK589_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ19798.1"
                     /translation="MSVVNIDQRHPAVLPHVRIQPAKGLLELDLAALWQYRELIHILV
                     WRDVTVRYKQTLLGVAWAMFQPVATMLIFTVVFSVMGKIPSDGFPYPVFLYAGLLPWF
                     YISQALSRGGTSLVGEAPLISKVYFPRLILPLSATIAPLVDLFLAFIAFLGLMAWFEV
                     MPTWRILFLPLFAGLGAMITLAAGLWVSALYVRYRDVGHILPMFIQLGMFVSPILYPV
                     SKVPESWRAIYALNPVVSVVEGFRWALIPGFQVDFSMVLPSLGIVFVVFIGGLIYFRS
                     MERTFADVI"
     gene            complement(30136..30663)
                     /locus_tag="PK589_09105"
     CDS             complement(30136..30663)
                     /locus_tag="PK589_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19799.1"
                     /translation="MIRRAIHLTAVLSLALSSACVSVQVDPLTTKAFEPRSGADAVTT
                     LQREPNHGHVQIARIVATSEYASEDTLRDRILERAKELGADAVVLGDADVRRLSDRGP
                     TFQSTMGTAVPGGVSNTNSYRSGYWNPFRMDAWSFTQGAGGGQGWMLHMSGLAIRYLS
                     AEEVRAAQKPPLSTP"
     gene            complement(30833..31189)
                     /locus_tag="PK589_09110"
     CDS             complement(30833..31189)
                     /locus_tag="PK589_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HNJ19800.1"
                     /translation="MKILAPCPSSPNCVSTQAADQSRQIAPYRYQQNLAGAQNTLKTI
                     INRLPRTRLVEENDGYLHYEFTSFLLRFIDDVEFVFDDATKMIQFRSASRTGYSDLGV
                     NRRRMEEIRTLVEGKL"
     gene            complement(31279..32481)
                     /locus_tag="PK589_09115"
     CDS             complement(31279..32481)
                     /locus_tag="PK589_09115"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNJ19801.1"
                     /translation="MDATSLWPAAGVVLVALATAMFLPRTTLQEASSTPRYPSLDGLR
                     GYLALAVFVSHSSIWYFYLRSGTWDVPPSNVYTQLGQGSVTLFFMITGFLFWSKLLDG
                     RHQPIDWSRLYLSRALRLGPLYLVAVACVLLMSFGRTGFQMREAPAVVAEQTAQWLLF
                     TIPGISSVNGFAETVPLAGAVWSLPYEWLFYAGLPLAAFCLRTSPPIQWVIVSLAAVV
                     ILVMVMPQVRFPILYAFAGGIAAAGLVRIAAIRMMLSRGVWGAIAILCLATAFMAFPT
                     AYTVPATLLLAGGFLIIACGNSLYGILEWPASIVLGEMAYSLYLLHSVVLFVTYRLLL
                     AESAATLSPAEHWAMVLCQVPALILLCSATFRLVEKPGMEAVPRWHARLVARRADTSA
                     SAVPSGRR"
     gene            32743..33918
                     /locus_tag="PK589_09120"
     CDS             32743..33918
                     /locus_tag="PK589_09120"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNJ19802.1"
                     /translation="MAGFPIEVATRIKTLPPYLFAAIDKMKQEALARGVDIINLGIGD
                     PDLPTPAPIIESLYEAAKNPKHHQYPSYEGMLSFRKAVADWYKRRFNVTLDPANEVLT
                     LIGSKEGIGHVHLAFIDPGDIVLVPSPGYPVYPVGTSFCGGVSHIMPLTKANGFLPDL
                     NAIPKDVAKQAKLMWLNSPNNPTSVIMSKDYFKRVVEFAQENQIIVCHDAAYSEIFYD
                     GKRPASFLEVDGAKEVGVEFHSLSKTYNMTGWRIGFAVGNKEVLAGLGRVKSQLDSGV
                     FEAVQAAGITALGLDDSVTDGLRKIYQERRDTLVPGLKKLGLEVDAPPAAFYIWVTVP
                     KGYTSASFTAHLLEKAGIVTTPGNGFGAPGEGYIRMTVCTTKERLAEAVERIRKVGF"
     gene            34010..34573
                     /gene="folK"
                     /locus_tag="PK589_09125"
     CDS             34010..34573
                     /gene="folK"
                     /locus_tag="PK589_09125"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNJ19803.1"
                     /translation="MATAFIGFGSNLGNRIDFCDRAVTLLGLLPHSRLDAVSSLYETE
                     PIEDGAAPGPAWFLNGAVQVETNITPKSLLEICREIERALGRDQDNRKGPRTLDLDLL
                     LYDDCVLNDPGLIVPHPRLHQRRFVLAPLVELDPDRRHPVLGQTLRELLETLADPSIV
                     RRLDPQPNSRYGSRPACSPPPVQSPHT"
     gene            34537..35427
                     /gene="panC"
                     /locus_tag="PK589_09130"
     CDS             34537..35427
                     /gene="panC"
                     /locus_tag="PK589_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNJ19804.1"
                     /translation="MQSTPCAESSHVKIIRTTSAMANWSRGLHREGVTIGFVPTMGAL
                     HEGHRALVRAARLACDAVVVSIFVNPTQFGPTEDLAKYPRQFRLDRALCAKEGVDAIF
                     APTATAMYPAGFQSIVAVPSLARRWEGEARPHHFQGVATVVTKLLSLVQPDISWFGQK
                     DFQQAALVRQLAKDLNLSGTLRVHPTIREHDGLALSSRNVYLSASQRDAAPVLYRALQ
                     AGAAAIRSGERRGALIQRLMTQMIKTERSVTPEYLAVCDPSTLEPLPTIQRHAILLGA
                     IRLGTVRLLDNMPVAIRRKP"
     gene            complement(35509..36018)
                     /locus_tag="PK589_09135"
     CDS             complement(35509..36018)
                     /locus_tag="PK589_09135"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ19805.1"
                     /translation="MKELYCPACGTPCVRVTSGTSLIEKTLNRLSIFQVRCQLCTTRF
                     QARRPGNRLTTQAFDRREYRRLPVNFAGTLRLDHTAAGGVVTDISMGGCTLQATTPLP
                     RGTFVTFVLHGPSGQPDIKVEAAMICSIQPQSIGMKFLEFQPEDKQRMGQLVLELLAY
                     QQAPPRISV"
     gene            complement(36020..36448)
                     /locus_tag="PK589_09140"
     CDS             complement(36020..36448)
                     /locus_tag="PK589_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNJ19806.1"
                     /translation="MMQPQDMVAEFHRKFDIPIGERPSIPPDATRQLRVRLIQEEFAE
                     LQEAMTAQDLPAVAKELADLLYVVYGTAVSYGVDMDPVFREVHRSNLSKVGGYKREDG
                     KWVKPPTYSPADVKPLLAKQLSGSVGSASHLETRSQESEA"
     gene            complement(36451..37860)
                     /gene="rmuC"
                     /locus_tag="PK589_09145"
     CDS             complement(36451..37860)
                     /gene="rmuC"
                     /locus_tag="PK589_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNJ19807.1"
                     /translation="MIDLTSTTFIAGGVAGLGIGVLVTGWWVTARTQSRIQAQLLESG
                     ERAQRADALAVALQQRLDTQQSEVDQLRQALAESQEGRTKAETRMEVISRSMEDQKAM
                     LSQARQELQESFEALSGQALKQNNAAFLNLARASFETLHAEAKGELSQRHQAIGELVK
                     PLEESLHRYQEQLQQAEQIRQREYGGLDQQLKFMAESHQRLQQETSNLVKALRAPAVR
                     GRWGEMTLRRVAELAGMVAHCDFLEQDSVDSMDGLLRPDMVVYLPAGRQIVVDAKTVL
                     AAYLDAYEAQDDQQRQAHLRRHANQVRTRMDALSLKAYWNQFGQTPEFVVLFLPGEQF
                     LGAALEQDPSLIEDGFAQGIVLATPTTLMALLRAVAYGWRQEQLTEHAEQAGRLGKDL
                     YERMAVLTDHLNDIGQALGKSVGAYNKAVASLETRVLPAARKFKDLGISSEKDLAMLE
                     STGIEPRTGFPFAAEDMRT"
     gene            complement(37956..40586)
                     /locus_tag="PK589_09150"
     CDS             complement(37956..40586)
                     /locus_tag="PK589_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNJ19808.1"
                     /translation="MIPSLILSLEESRDPSLVGGKAAGLNRLLAAGFAVPRGLCVPTT
                     VYRHVLAAGGVNAEEVWRRVLQGSQEERSQELARVHACLLKQAWPTGFQIALERRLVE
                     LTHDPTTRWAVRSSATNEDVEQASAAGLYSTSLGLSSSEILRGICDCWQSLWSDAAVQ
                     YALRTSSSKSFPSMAVVIQPLLQATTAGVAYSIHPVTGRTSQVVVNAVPGLAASLVSG
                     EVTPDQYVVEVAEDEGRPIRIRRRLLARKVQKLLSTPAGLHTESISEAGQKQSSLSDA
                     QLFELATLSKHIEAAFGHPIDLEWVWDADKVWVVQARPITAVQPSPALTNDECEWTRA
                     NLKETMPELPSPLGLSFLERFMDAYIVGPYRRLGCTVPEGLSSVRVMHGRPYLNVTLF
                     YTLVTQLHGNPSFLTEQMGGEPLGFTPSVRPLSGLALVRAGVLMMREWRRVVRQGPAN
                     FVAMKDMAQTYRPDRIRNLSAKELAATLDRLGQWLDTHEVTFGIAGGVAQSLQAMGTF
                     LPGWLGEDWRELLNASLQGQGSVISAQHILRLAELVEVASRESVVQRWFLREGWAASG
                     FREALAGTGFLRRFDEYLADYGHRAVGESDIMSPRIADQPDSVLMLLRTQLREGKAVP
                     PGDVMTQQAQRREAALAEIYRRFGWRRHRGVVFRWWYRRLSRFCALREANRHHLLYYS
                     TAVRHLLLQLGERMVEQGILTQPDDMFYLTMDERVALNEGTSRDWQRLVRTRREQRTR
                     DQNASVPDTVRDWEAVLEQGPSMTGQVDGGLLRGIAVSAGTTSGPVRIVRSTQDWQRV
                     RPGDILVVPVIDPGMAPLFGIAAGLIAEMGGTLSHGAIIAREYGLPVLVNVPYATTLL
                     REDEPVHIVSSTGTISRLVP"
     gene            complement(40602..41942)
                     /locus_tag="PK589_09155"
     CDS             complement(40602..41942)
                     /locus_tag="PK589_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNJ19809.1"
                     /translation="MIEIGVPQPRTRALLARLLQAGLHAVDPYEAVRRQVSIGRRHLT
                     IGSQQYSLNQVKRIVVVGAGKASARMAQALEHQLGPRIDAGLVVVKYGHGAPTNTIRI
                     VEAGHPIPDVAGLEAGRQVMAVVKTLTRDDLLIVLLSGGASSLLPAPVSGITLQDKQQ
                     TTKLLLRSGATIHEINAVRKHLSSVKGGQLAAATAARVASVILSDVIGNDLGTIGSGP
                     TAPDPTTFREAWEIVERYGVVKTIPASVRNRLSMGMRHRAPETPKPRASVFRRVDNIL
                     IGDNQAAVDAVATAAQQQGLRTLVLSTTLTGEARELAKMFGAMAREMARHGRPIPRPC
                     CIIAGGEPTVTIRGEGTGGRAQEFALAAAPEIAGLPNVWVAGFATDGTDGPTSVAGAV
                     VDGQTVVRARRKGLDLSQALQDNDAYPFFEKLHGHIVTGPTGTNVNDLYLLLAL"
     gene            complement(41948..43024)
                     /locus_tag="PK589_09160"
     CDS             complement(41948..43024)
                     /locus_tag="PK589_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNJ19810.1"
                     /translation="MRNNDWGLRAFVVGVTMVLGLAPLFAEVSLDSKAPAPSSPSASD
                     DSRVPIPERGTGPLSAEPEGAPELEDRLVILPEIKREGERFFLSSFKLPDKLTFAGQS
                     IPLDNWQVRERIEYEFYQFLEDQGESIILAKRTGRCFAPAEKQLADAGLPDDLKYMLL
                     VESKCIAAAYSRAKASGPWQFINSTGKRYKLHNERWLDERRNLEMSTEAAIKYLRYLR
                     ELQGDWFLAMASYNAGEDRVRKLLKEQNVTDFWRMHGPRETMRYVARIIAAKEIYSQP
                     EKYLGLTKKDLYPPLETETVTVTIKEAQRRLTSIAEEYGTYFLELKMLNPEFTKDYLP
                     KGSYQVKVPRQTCPNRCFKQDKLP"
     gene            43119..43826
                     /locus_tag="PK589_09165"
     CDS             43119..43826
                     /locus_tag="PK589_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNJ19811.1"
                     /translation="MSTLSMLNADIIACTQCPRLVSYRESVAATKRRQYREWLYWGKP
                     IPGFGDPRARLYVLGLAPAAHGGNRTGRVFTGDRSGDWLYEALHRFGFANQATSTDVD
                     DGLALTDCYIGATVRCAPPANKPTPDEFTACRPFVLGELRLLEHVQVVVVLGKIAFDH
                     YFKACREMGRELPSPLPKFGHGVAYELAWGVSLIGSYHPSQQNTFTGKLTRPMFHRIF
                     SDAASRLEGNRRGSPSG"
     gene            complement(43833..44480)
                     /gene="fsa"
                     /locus_tag="PK589_09170"
     CDS             complement(43833..44480)
                     /gene="fsa"
                     /locus_tag="PK589_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNJ19812.1"
                     /translation="MKLYLDTANVKEIQEGANLGLIDGVTTNPSLVAKEGRSFKEMLL
                     EICNMVDGPISAEVVGVESEAMIKEGRDLAKVHKNIVVKVPLIPEGLRATKKLSAEGI
                     RVNVTLCFSPTQALLAAKAGAWCVSPFIGRLDDVSSDGMALIRQIVTIYKNYDFKTQV
                     LVASVRHPQHVVEAALAGGHICTMPYSVFQQLVKHPLTDIGLKKFLADWDAMGKK"
     gene            44654..44956
                     /locus_tag="PK589_09175"
     CDS             44654..44956
                     /locus_tag="PK589_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19813.1"
                     /translation="MRASVWLLAVGLLVGACSHNPYLDNSLTRYQGKDRAWIEKELGP
                     PDAKTTRFFGGEKWTYNRIAGGKAGPPLFNFKANECQMILFFDKDDTVSDSTYSGC"
     gene            complement(44953..45213)
                     /locus_tag="PK589_09180"
     CDS             complement(44953..45213)
                     /locus_tag="PK589_09180"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNJ19814.1"
                     /translation="MYRFFLIAVLLIVLYYLIRRAIRKIGQDGALEKPKDGRLSGNQM
                     VQDPVCRVFVARENAVKENIGGQTYFFCSRGCAAAFEKQLSG"
     gene            complement(45892..46695)
                     /gene="dapB"
                     /locus_tag="PK589_09185"
     CDS             complement(45892..46695)
                     /gene="dapB"
                     /locus_tag="PK589_09185"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNJ19815.1"
                     /translation="MIKVVITGAAGRMGSRLVSLVKDSAFLTLAGAVESKGHHALGED
                     SGEVAGCGRTGVPIVEDLSSLMERGEVVVDFTTPAATLGHLRIVAQHRRGIVVGTTGF
                     SPSELDELRSVSKQIPCVFSPNMSVGVNVIYKVIAEMAKTLGEDYDIEVIEAHHRLKK
                     DAPSGTALKMAEVLARAVNRDLGQVGVYARKGLIGERKKGEIGIQTIRAGDIVGDHTV
                     MFGTMGERIELTHRASSRDTFARGALRAARWLVKQPPGLYDMLDVLSLK"
     gene            complement(46725..47597)
                     /gene="dapA"
                     /locus_tag="PK589_09190"
     CDS             complement(46725..47597)
                     /gene="dapA"
                     /locus_tag="PK589_09190"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNJ19816.1"
                     /translation="MFTGSLVAIVTPFKNGKLDERALGDLIEWQINSGTHGIVPCGTT
                     GESATLTHAEHDRVVAFTVEVVRRRVPVVAGTGSNSTEEAIALTKHAKAAGVDGALLI
                     TPYYNKPTQEGLFLHYKAVAEAVELPLVVYNIPGRTGVNMMPSTIARLTVCPTVVAIK
                     EGSGAVQQASEIIQLCGERLTVLAGDDALTLPMMAVGGKGVITVTANLVPTKMAELVN
                     AFRDGRLEAARAMHYELYPLFTALFYETNPIPVKEALHLMGKIAPEIRLPLCPMGNDN
                     KAKLLTVLKAAGLV"
     gene            complement(47634..48896)
                     /gene="lysA"
                     /locus_tag="PK589_09195"
     CDS             complement(47634..48896)
                     /gene="lysA"
                     /locus_tag="PK589_09195"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNJ19817.1"
                     /translation="MHDFQYRNGELYCEEVPLSRIAKEVGTPCYVYSHHTLVRHFRVY
                     DGAFKNIPHIVAFAMKANSNLAVLRLMAKEGSGVDIVSGGELFRALKAGVPASKIVFA
                     GVGKKQEEIRDALKADILMFNVESSAELQAINEVAASMDVKARVALRINPDIDPKTHP
                     YISTGLKKSKFGIAADRAIEEFKNAAAFSHIKVVGLHAHIGSQLTQVTPFVESLKKVL
                     TMVQTLAEQGIPLRYVNIGGGLGITYSDETPPEPKDLAEAISPLVRDLKCVLIMEPGR
                     VIVGNAGVMLTKVLYAKDGEAKRFLIVDAAMNDLIRPSLYDAYHDIRPVYEKVTQGEK
                     KTVDVVGPVCESGDFLAKDRVMPAMGAGELMAVMSAGAYGFVMSSNYNSRPRVPEVLV
                     HEGQYHVIRSRETYEDLVRGEHVPEFLS"
     gene            complement(48996..49298)
                     /locus_tag="PK589_09200"
     CDS             complement(48996..49298)
                     /locus_tag="PK589_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19818.1"
                     /translation="MMSAGARRAAIQPHALLATVSFILLAGCGVLGAPIPPEDVGVAP
                     VVERQLRREGLLASGANVRGSASRPSAPSGLTIEETPIPPDPDPLPVSPWRTMGTR"
     gene            complement(49295..>49954)
                     /gene="argH"
                     /locus_tag="PK589_09205"
     CDS             complement(49295..>49954)
                     /gene="argH"
                     /locus_tag="PK589_09205"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNJ19819.1"
                     /translation="STLSLIMMHLSRLSEELILWASQEFRYVDLPDTFCTGSSMMPQK
                     KNPDVPELVRGKTGRVYGHLMGTLTLLKGLPLSYNRDLQEDKEALFDAVDTTGQSLVL
                     CTELVRRLAVNRDVMAEAAEGGGMLATELADYLVTTGVPFREAHSITGQIVRFSLEKR
                     RPLQRLTLKELQGFSSHFKADVLNCLTVRGAIDRKAQIGGTARRRVEARIKELEKALK
                     G"
BASE COUNT        10869 a        15620 c        13519 g         9946 t
ORIGIN      
        1 ttgacgtaga tttcttccgt ggccggttca atatagtccc accgcaactt gccgggcttc
       61 ttgatgtaaa agtgaccggt cgaaatcacc ggcgtggaga atccttcaat cctggttttt
      121 tgcgtgaatg acgcctgcaa gtccttggtc ttttcatatc gcgactgaat gttcttcacc
      181 acctcctgca cttcgtggag cgactggagg tcgacggccg gctcgtccgg cttcacgagt
      241 tgagccgcag ccggaatggc gctgaccact aacaccgcaa tgagtaaccc tgttcgcatc
      301 atgcctcatc tccaccaacc gggcctcgac gtccgagcac ctcgcgacgt ccatcccgtc
      361 cggccgcgcc cacaacgcct tccgcctcca tctgttcgat catgcgcgct gcccggggat
      421 agcccacccg caagcgccgc tggatcaacg aggccgaagc ctggcctgtc gagaggacga
      481 ggtccttcgc ttgttcatac acttcgtctt tcgcctgctc ctcttccgct tcctcgatct
      541 tgagcgattg cagttcgcga caatacgagg gcaaggcctg cttctttaca aactcgacca
      601 ctcgccgcac atcgtcgtcc gagacaaacg atccgtggat acgcatcaat ttcccggttc
      661 ccgacgcgag gtacaacata tctccgcggc cgagcagcgc ttccgcgccg ttcgcgtcca
      721 ggatcgtgcg ggagtcggtt ttggacgaga cttgaaacgc gatgcgggcc gggaaattgg
      781 ccttgatgag accggtcagc acatcgaccg acggccgttg tgtcgccagt accaggtgaa
      841 tgcctgaggc gcgcgccatc tgagccagtc tggcaatctt gtcttccaca tctttcgggg
      901 ccaccatcat gagatcggcc aactcgtcga tcatgaccat gatatagggc aagggctcag
      961 gcaggctcgg cttgaccgaa tcggtcggcc cgttgtcgcc ggctggctct gcatcttccc
     1021 ccttggaaag ccgttcttct tcggacagaa acgtcaattc cacctgttcg gctttcccgg
     1081 attgccagac atcggacacg gccccatgga gctctgaaat ccgccgatta taggcgtcga
     1141 tgctgcgcac gccggcttca gccagcaact tgtaccgccg ctccatttcc tgaaccaccc
     1201 atcccaggcc acgcgcagcg gacttgggat cggtgatcac gggacgcagc aaatgcggaa
     1261 tcccgtcgta actctggaac tccagcattt taggatcgat gaggagcagc ttgacttcat
     1321 ccggccgcgc gctgaacaga atgctgagca acatcgtatt gagaccgacg ctcttgccgg
     1381 caccggtggc ccccgcgacc agcagatgcg gcatggtctt cagatcggca cagactgcgc
     1441 cgccgaaaat gtctttgccg agcgccagac tcaatttcga gcgggagcgt gaaaaagcgt
     1501 cgctggtcac gacctccttc atggacacca tttcccggtt cgggttcggc acttcaatgc
     1561 cgacgacgga cttgccaggc aagggggcca cgatgcgcaa actgatcgcc ttgagcgcaa
     1621 gggccaggtc atcggccagg ttcacgatgc gcgcgacctt ggtgccgggc gccggctcga
     1681 actcatacat ggtgacgacg ggccctggac gcacttcggt gacgcgtccc tcgatcgcaa
     1741 aactcttcaa ggccttggtc aggatctccg attgaagttt caattcatcg tcgctgaggc
     1801 gagcgatagg accggacgga tcactaagta attcttgcgg atccggcaac acatatccgt
     1861 cggacacgga tggactcgtc gccaccgccg cagccggttc gtcttcaggc gtagcacgct
     1921 tctcgacttt catcggcggc tgaattttgg gaggaatcat ggggaccgcg atttgctcaa
     1981 cagcctcggc caccacctgc acttcgcggt cgagttccgg ttcttccatc tcacgtgcgg
     2041 ggcgtgcgac tttcacacga gcccgcttcg gtggcgccat cggctcttcc tgcttgaccg
     2101 gccactccgg catgagcccc gtcatccgct cacgggcggc gatccaccag tccggaatcc
     2161 tctgcagcaa tgccgttagc gacacaggca ccgtaaagag cagggccacc atcaaccccg
     2221 tgagcaccac aatatgcgcg cccgtcgtgg cgaaataact gcgcactcca tcggccaaca
     2281 cctggccgat gatcccgccg gctaagccgc gattgaccca tccgctggaa atcgtaggca
     2341 ccgctgtgac ttcgaggtgc aagagcgcgc tcaaaaacac catggccgcc agtccgctgc
     2401 ccgcattcct gagtcgcatg gtgaggggga tgggagtgaa acagcgggca ccgagaagcc
     2461 cgagcagaat cggaaagagg taggcggccc caccgaccat gaagaaacat gcggccgccg
     2521 acagcgctcc gaccgacccg atcatgtttt tgggctgatt gccggctgct ccggatgccc
     2581 caatcgattt ggcgtcgccg ggaacaaacg acaccagact gagcaggatc aagagaccga
     2641 ctgcgatcag gagaaccccg atcacttcgc gcttcacatg agaggaacgg gaaggggctg
     2701 agcgggcgtc accgcgcttt gccgtggtgg ttacacccat gcggcggatc gtagcacagg
     2761 gaaaatacct tttcaaacaa agcggcaatg ttgcgcatgg ctatcacgat gctcccattt
     2821 ccgtcgagac cgtcgtgatc aagccgactc atcatcgtcg accgccgtag cgagggcgcg
     2881 atagtcatcg agggtcagcg tctccgcgcg agcctgcaac ggcacaccaa cggcttgcat
     2941 cgcccgggaa atccgctcga ccggataacc ctcgtcgcgc aacgaattca ccaacgtctt
     3001 ccgccgatga gcgaaggcgg cccgcaccaa ccgccggaac cgctcatacc ggtccggatc
     3061 gacgccgtcc tgggtcttga gcttgagatg tacgactgcg gatccaacag ttggacgagg
     3121 gcggaaacaa ttcgccgaca cgcgaaacgc cacctccacc tctgccgcct cttgcgtcag
     3181 gaccgacaac acgccgtagt cttcgctatt cggtttggct gccagcctga gcgccacttc
     3241 cgtttgcagc atgaggacca gacgatcgag atgggcacgg gcatcgagta acgcaaacag
     3301 gatcggcgtg gacacataat acggcaggtt ggagaccacc accgtccgcg gcggcaaact
     3361 atcgaaggga aattccagcg catcgccgat acgaaggtca aggttccggc aatgactgag
     3421 cgtctcctgt aattggggct gcagttgcgg atcgatctcg actgcaatga cgcgtccggc
     3481 ttcggcgcac aacccggccg tcagggcgcc acgcccgggt ccgatttcca aaaccgtgtc
     3541 gtcgggacga agcgcggcca gggatacgat tttgcgaata atgttcggat cgatgaggaa
     3601 attctgacca agccgtttca gggcgggggg aaaggaagca gccatgattt cacgcggctt
     3661 tctatccggc gttgggtggc accgccgctc gcaggcgttt ggccaatgtc acgagatagg
     3721 accggtaaga ttcgacctcg tcgctgaact cttcgatcag atcgttggta aacgacatcc
     3781 cgggcttctt ccgggacagg ccatccgcct cagcctgtcc ggcatgctgc tgaagcaacg
     3841 ccaccgtgcg aatcttccac tggcccacac gttctgtgcc cagggtggtg ttgaagtcct
     3901 ggatggtccg cttttcgatt ccgtccaact ccttcacctg ttggtcaatg atggttgctg
     3961 cggaagagcc tgcgtctgac atggagatac cgcctctttc ctatcgggac aatgaatgaa
     4021 caggggacca ggatttggcc aaacgagccg ccaccttcac tgcctcgagc agacttccat
     4081 gctcggcgac ccctttgccc gcaatatcgt acgccgttcc atggtcgacc gaggtgcgga
     4141 taatcggcag gcccaccgtc aaattcacac aggtgccgaa ggccaccaac ttgaggggga
     4201 ttaaaccttg atcgtggtac atcgccacaa ccccatcata atcgccgcgg gcggctttgc
     4261 cgaacagggt atcggcaggc agtggatcgc tggcgttgat cccggtcgcc cgggccaatt
     4321 tgatggctgg cgcaatactg gtctgctcct catttccgaa taagccgtgt tcacctgcat
     4381 gaggattcaa cgccgcaacg ccgatcttcg ggcgtgcgat gccgaaatac ttcgtcaggc
     4441 caagatgggc cagacgaatg gctttggcaa tgcgctgcgt ggtcaggagc ggagacaggg
     4501 catggattgc gacatgggtc gtggtgaaca taatttttaa gggcccgccc acgatcatca
     4561 ttccaaattc tttcgtcttc gtcagatccg ccagcagttc cgtgtgcccc gggtaatgaa
     4621 agccggccat gttcatcgct tctttattga tgggcgcagt gacgattccg cccacgctcc
     4681 ccgcctgcgc caattcgacg gcttctctga taaatgcgat ggaggccgcc ccggtcactg
     4741 ccgacgccac gcccaggcga aatttcggca gcggacgttc caacggatcg gcgacagcga
     4801 cctgaccggg cttcactcgt gcaccgctcg ccggatcaaa cgtcaccacg tccaacggca
     4861 aatccaacca cttgatcgtg cgttccatga ccgggcggga gccgatgacc accggctgac
     4921 agagccgcgt gagcgccttg tcggccaagg ccttcgcaat gacctcgggg ccaatgccgg
     4981 ccgggtcgcc catcgtgaga cccaacacgg ggcggagcga gtgttgtgaa cgcttacgag
     5041 ccatgggtgt acaagaataa cgtgtaccca aggtacagca gcgcgctgcc gctgagcaac
     5101 caggggcgcc agtgccctga ccacagggcc tgtaacaggc tcatgctcat cgccacgacc
     5161 gcaaggacca tcgtcgccag tgccgttgta cccaaggtcc attcggtccc aagcaatccg
     5221 acggagacgg ggatcgatcc ctggaacacc atcgcaccgg tcacgttccc cacggccagc
     5281 cgatcctttt tccggtacaa ccacaaaaag ctattggaca tctcaggcaa ttccgtagcc
     5341 agcggagcga tcaacaatgc gaggatgagc ggcgagacct ggagttgggc agaaatggag
     5401 ttggcagccg tcacaaacag atgagcgcct ccaaccaacc ccagcaaccc gacgatgcct
     5461 tgcgccccga tcaccagata ggagggctta gcggagcgcc gcgcgaagag cagcggctcg
     5521 agttcgtctc cgccctcact ctcttcgtcc tcggcagaaa acttgagctt catgtaatag
     5581 acgtacatgg ccagcaggac agccgccacc gcgacatgca agacccggga gggaatcatc
     5641 gcgcacgtga gcgccaggac atagctgacg agaaagaagc tgatatcgac ccgcacctcg
     5701 ccataattca gcttgaacgt tgctgttcgc ttccccaact tggcatagag caccagcagg
     5761 agcgccagaa tcggcaagac taatgtgctc agcatgaatg gcgccccaag aatcgctccc
     5821 agacccactt ccatctgttc gcggccagcc ccgaaaaaaa tggcaatgat tggaatcgat
     5881 gtttcaggca aggtcgtccc gacggcagcg aacacactcc cgacagcgcc ttcggagata
     5941 ttcaggcgtt ttccgagcca ctcgatgccg ttcgtgaaga gatgacaccc ggcaagggtg
     6001 atgacgacgg aggccacaaa cattcccaca tacaggagaa ctgtcatcgg cgcctccgcg
     6061 ggatcagaaa aaaggggaag gagagaacag ccacgatctg gtgaatcacg cgcgcccttt
     6121 tcttctcggg cgggtgccta gtgtctgccg atacaccatc agggcttcgt cgagaatgtc
     6181 tttgacggga cgaccactcc gttctgccac gcgtttacaa tcgtcatatt ccggagcggc
     6241 tttgctgagc cccggccgaa tatcagcgag ttttatccgc acatcctgtc cgttgactcg
     6301 caccgaagca aattggcgcg gcaacacacg gcgttgcacc tcgcgaatcc gcacaccgag
     6361 agccgtcgtc tcggcaaaga gcacagccag gaccgcctct gtgtgttccc gcggcgcaag
     6421 gacggacagg acattccccg gccggccctt cttcatgatc accggcgcca gggtcgcatc
     6481 cacggcgccg gcggcaaata cgcgatcaaa caccgtgtcg tacacttgcg ggttcacatc
     6541 atccagattt gtctccagct cgacgatggt ttccatcgcc ccgctctcgg acgcctcgtc
     6601 accgatgaac acgcgcaaca cattcgccca ctgtgccggg tcggcagtcc ccgccccata
     6661 tcccacctgg cgtacctgca tggaaggcag gcagccgaaa tcggtggtca cggttttgag
     6721 caaggccatc ccggtcggtg tcgtcagctc ccgttcaggc cccttggcat aaatcggcac
     6781 gccgaccgcg agggccgcca cggctggtcc cggcacgggc aacgcgccgt gcgccgactg
     6841 cagcatgccg gacccgacat tgatcgctga ggccgttaca cgccggatat cgagaagatg
     6901 caggcccagg atcccaccga cgacatccac aaacgaatcg atcacgccca cttcatgaaa
     6961 atggacatgc gacggctcaa caccgtgggc cttcccttcg gcgtctgcca gcacatcgaa
     7021 taccgcctga cttcgttctt tcaccgctgg cgggaggccg ctcctgcgaa gaatcttggc
     7081 aatttgtgac agggttaacg gcgcgcgaaa gcctttgtcg atcaggacat caaccttggt
     7141 cgccgtgatg gctccgcgct caactcgctt acgcgtcagc cgatagccgt cgatcttcag
     7201 cgaagcgagg cctcgcacaa gatctttgaa cggcagcccc gcatcgacca acgcgcccaa
     7261 tgtcatgtcg ccgctaatgc cggagaaaca atcgaaatgc agatgcctcg ccacgtcgct
     7321 cctacaattg actctttcgc gcaaacgagc ggcatcttac cggagccgca cccagacagc
     7381 aatcattccg ctcgttcgtt gacagccctg ccatgctgtg ttatccccaa tcaagagacg
     7441 cattccatcg gccaccagcc accaccacct gcgacaccga cacccatgac gaactcaaca
     7501 ccagctcccc gggtcagcct caaacgcgca tcgacaggaa ggactttcgg cctgctgctt
     7561 ctgctcagcg gcttttgctt actgggagac ccaagtctgc ttctcgccaa aaccaaaggc
     7621 aaaagcagtg tcccgcgccc cgagccggac ctaaaaattc tttcgctgca agctgcgccc
     7681 atgccgtatc gaccgcagga gggtcctttc catttcatcg ccacggtgca actgccgaag
     7741 gaagtcgatg aacatctcat gttggaagtc tccgccctcg tcacctcttc ctctatgacc
     7801 tcgctgcgct ttctctcgat tcgacagccg gtgaatgaac atgtccagac cacgccaagc
     7861 gccaatggcg atacacccca aaaacatgtg caggtcgacc tcgaatggga cgggctggat
     7921 cacaacaaac aacagatccc gatcggatcg tatgagtatg aagtgcgggt caagctgttg
     7981 agtaacgggg agaagggcca gcgcacccaa atgctgtcat ggcccaaacg cggcaagatt
     8041 gtggtcaaac actagacgag cacgaggctt cgatacgaca ccccgcctct actgacgctt
     8101 tgccctttca ggcttaggac tatccatctc actcaccatg ttgatgcggt gggccaggca
     8161 gccggcgccg aacccgttat cgatattcat caccccaacc cccgccgcgc aagaattcag
     8221 catcgtcagt aacgcggcca ggccgccgaa atgtgcgcca tatccacgac tcgttggcac
     8281 cgcgatgacg ggctgccgta ctaatccacc gacgacgctc ggaagcaccc cgtccatccc
     8341 ggccgccacg atgatcgccc gcgcgtcatg taaccggtct tgttgaccga gcaaccgatg
     8401 caaccccgcg acaccgacat cgtacaaggt gtcagtcttg ctgcccatga tatcggccgt
     8461 cacacgtgcc tcttcggcaa cgggaatatc ggcggtgccg gctgtgacga tgagcacaga
     8521 ccctcgacgc accagcttgg gcggcacaat ggccaccact cgcgcggcct catgatatgt
     8581 cgcccgcttg ctcatgcgga gcaatgcgcg cgcgacggca ggttccaccc gcgtcgcgag
     8641 caacgaatta tttttcagca acagtgtctt ggcgatagcc accacctgcg ccacagtttt
     8701 cccttcgcaa aagatgactt ccggaaaacc ctgccgcaga gcacggtggt gatcgagcga
     8761 tgcaaatcct aaattctcgt acggcaacgt gcgtaaccga tgtagcgcat ccggcaccgc
     8821 cacggcgccc tgctgaacct ccatgagcaa cgcccgcaac tgatcttgat tcatgagagg
     8881 gccttctctt ccttcgcttc gcgttccatc aagtcaccga ctgcctggcg gcagggtttg
     8941 tcggcgaaga gaaccgcatt gacctcctgc acaatcggca tatccactcc gcaccgttgc
     9001 gccagcccca atgccgcctt ggccgtacgt acgccttcgg cgacggcctg catgctggcc
     9061 agaattcctt ccaaccgttc acccttgcct aaccgtaccc cgaccgaatg gttccgactc
     9121 aacgggccgg tacaggtcag gatgagatct ccaaccccgg acaatccgta aaacgtccgc
     9181 gggtctgcgc ccatcgccat gccaagccga atcatttcgg ccaacccgcg cgtgatcaag
     9241 gctgcacggg cgttatggcc gagttccagc ccgtccacca caccggcagc cagcgccatc
     9301 acatttttca acgatccgcc gagctggacc ccgatcagat cgtcatctgc gtagacccga
     9361 aacgtcggcg tcatgaagag cgcctggatg gctttgacgg ctccggcgtc ctggccggcg
     9421 aggcagagcg ccgtcggttt tccctggctg acttcgctgg cgaaactggg accagacaag
     9481 accatcaatg aatgtcgcat atggtcaggc aggacatcct gcatcacctg cgtcatcagt
     9541 tcgagcgtcg tttcttcgat gcctttggtc gcgctcacga atggaatcga ctgtggcagg
     9601 agagggccaa gccggctcag aaccagtcgc gccacatgcg aaggcaccgc gaagatgaca
     9661 caatcagccc cgatcagggc gtcggacaag tggctggtgg cggttaaggt agccggcagc
     9721 cgcactcccg gcaaaaacac gacgttttca cgtcgttggt caatggcctg cacgacctcc
     9781 ggttcatgcg cccatacaca cacggagatc tgttgctcgg ccaaatgccg ggccaatgcg
     9841 gtgccccagg cgccagcgcc gatgactgct acacgttgac tcgatgatcg cgtcatggac
     9901 gcggattata ggaaggggtc tggaagggtg tcaacgaacg catccggcac attcgcatcc
     9961 acactggaat tccgtgcgcc cacacggtag gctgcgggtc cacgaggagc ctgagccatg
    10021 aaaccttgtc cagcctgcgg acgcgccctg ccagaaatca atcgctactg cacctactgt
    10081 gggaccggcg tggccagcga cacggcacaa aaggcaatgc cgcaagcttc tgaggcaacc
    10141 agagaccagc tcaatctcca catcctgtat ggcatggtca cggtgttggt cgtgtctgtc
    10201 atcatgccgc cctgggaaac gccgccctcg caaccggcag cctttctcgg ctttcacttc
    10261 atcctttcgc cgccacaacc cgatgccatc gtcagccgtt tgttgctcac gattgaactc
    10321 acgacgacgg cgattgcggg gctgtaccta tcgtttcttt tccgatcgaa ggggtaacgg
    10381 tgaggcactc aggcaggcgg gagagtgctg tcagtccagt gcaagagtca ggcatttggc
    10441 agcaccgcct gacttcatga actcatccag ctccacatca tgggtgcgat agccgcggga
    10501 ttccaggagc cgcatcgtgc ggggacagcc ggccggaatg acgatacgct tgccgacgca
    10561 gaccgcattg caggcaaacc gaatcgcttc gtcttccggt acgacaagcc gctgttcctc
    10621 agggatccgt tgcgcgatcg cggccaggcc ataggcatcg aaggcggaag gaaagtacaa
    10681 cagctctccg ccgctgagcg gacaaaagca ggtatccagg tggtagaacc ggccgtcgat
    10741 gagttcgagc ggaatgatct cacgctgaaa aatttcgctc agcttcggaa acgaccggat
    10801 atccgatcgc tggcgatatc cgccgaacca tgtgtccgga aatcccaaga gatcgccggc
    10861 gccttcaaaa tagagcgatt cctcgagcgt cagcacctca tacccatgct cgcggaacca
    10921 ttgcgcgaag tacacctctt cacgccggcg ttcgggataa cgaaaccggc tgatcacaac
    10981 ccggcgactc gccaccaccc cggcattggc cgtaaagacg agatccggaa ggccgggaac
    11041 cggctgcatt cgctcgacgc ccactcccac atcctgctcc aggacgtgca tcagcgtctg
    11101 ccattggccc accgcgcgag aaggaaccac cgcattctcg cgtcgcatcc agggattgat
    11161 ttcgtaatcg atttggaaat attcgggcgg acacaccagc agccggctca tagacgccac
    11221 ccgagttcac gcgccagctc cttgagaaac gaggcggcgt ccatcacgag accgacggct
    11281 tgaaaactgc cccggtccgc cagttttgtc ggcacggaag gattaacatc gacacacacg
    11341 gtagggacgg aggcgggcaa caaattgccg gtcgcaatcg cgtgcaacgt ggaggccact
    11401 aatagcgcaa ggccgacgcc ctgcacttcc gcgcgcatcg ccccctgagc ctgcatggaa
    11461 tccgtcacca cgccgggcaa tggcccgtcg tctcgaatcg tgccggccat caccatgttg
    11521 acgtcctggc gcacgcaggc ggccatgatg ccttccgtaa tcacaccgga acgcaccgct
    11581 tcatggatac tgccgatcgc gcgaatccgg ttgatcgtcc gcaaatgatg ctcatgtcca
    11641 tgcggaacgg aacgtccagc agtcaatcca tatccgagag acgtgccgta taacgcggct
    11701 tccatatcgt gcgcggccaa cgcattgccg cagaacaaga catggataaa tccggcttcg
    11761 atcaaccagg tcagcgcttc ccggcctccg gcatggacaa ttgccggtcc gcccgcaagc
    11821 agcaccttgg tgccggtctg cccctgccga tagccggcgc gtaatttcag catgcgttgc
    11881 gcgatatcgg cgatgaccgg ctgatgcggc cgctctgagg acaccacaga ttccatgaac
    11941 ccgaacacat cccgttcgcg gggacgggcc agcggcgtca ccctgatgcc ttctctcccc
    12001 gtcacgatca agtccccggc tttcaccgat cccatgggca gcgttctggc ccgagccttt
    12061 gccgggtcga cacaaatgcc gagatccatc tccatgccct cgacctcaac ccactcgcca
    12121 tcaaggcgga tctgggtcgg caaatgcgtc gtggcataga actcgtccgg gaatatcccc
    12181 gccgccggag ccggctcgaa acggcactcg gtgtcccgtt caaccgcggc gccgtggggt
    12241 tgaatggctt ggagaatctc ggccaacagc cggctggaat gggcgcgcac gacaatccgc
    12301 gcctgcgagg gcgcatctct cgtcgcccca atgttcacat cctgcaaatc gaacgtgccg
    12361 cccatcatga ggatcgtgtc cagcaccttg gccaggatca acgaatcgat gatgtggcct
    12421 tggagaaata ccgtttcctg cagtgcagtc atgacggatc tcctgaacgc tctccggcag
    12481 tggcagcggt atcgaccctg cgtaacaacc gcgtaccatg attctaccat tagtcaggaa
    12541 ggatggcccg cactacgcat ttgtcttcac gatctctcgt agcacgctcg tggcatagga
    12601 accgggggga agccagaacg agagggtcag caccgacccc tccagcgacc agaccagatc
    12661 attgagacgc acgcgcaggg ctcgccgttc accgcgaaat ccgcactcgg ctccggcttt
    12721 cgagagcact tccggcgtgg ttcccagttc agcgatgacc gctcgctcaa tctcgcccgg
    12781 ctcaccggta gcccagggtg cgcgcgaacc gaagagtggt cccgtcgggc tgatttcgaa
    12841 tcgatccgcc cgcagctgct cgacctccgg ctgctccaca ggaaagcagg cgccgttgtc
    12901 ggacttcatg gcccaatcac cgagcagcac ccggtcaata ctgtcgattc gcttcgccac
    12961 aatgtgattg aacacatacg actgataggc attcatgaac cagatgcgct tggcgcgagg
    13021 catcttgttg cggcgggccg catcctgcag caattcggca cccagctgaa agttcgtccc
    13081 cgagcgtcct tgccgttgcg ggccgaaata gttgggcaca ccgcggcgca ccaactcctc
    13141 gaatagacgg cctaacaatt cctcacttcc ctcgcgaaca tcccgcacga ccaaacggaa
    13201 ctgattgccg gcatggtgcc ctttacgcaa tcgattgcga tggcgcccca atacctcgac
    13261 gctcaggagc cgttcgtcgg tcggtaacgc cgcgaccgct tccgccttca caccctgtaa
    13321 ggacaacatc tgcgtcgtca cggcctgcgc gtccttcagc cctgccacgc cgatcgtctg
    13381 cgccttcaca tgcaggtggg acgagagtcg cagcacaaga tccggggtgg acagccctcg
    13441 cttggtgatt ttgatgtaca agtgctcgcc ctctccgcag gggagataca gggggcgctc
    13501 atccacacaa aagtcttccg gtgtactgcg aatgcgtccg ccgagcgcag ggacagacgc
    13561 ggtcaaatag ggtaccgtat catcgagcca cgagggagtc ggcatgagtt tcctttatga
    13621 ttcctgaaag attacacttc atgtcatttg gttaggcacg gcgaccaccc gtgttatact
    13681 gctttgagat agtctccacc gccaaccatc ctgctggacg cacttccgat gcgtagacgc
    13741 gttgttcata ccagctgtgc gatcatgttc ttcttctgcg cctgcacggt ctcgcaggcc
    13801 cgatcagcga cggcggccgc ccgaccaatt ggaatcgacg tcttcgccca gctcacccag
    13861 cacattaaac gtcaggctga caccgacaaa gaggcctttg cgattgccag ccaatgcatg
    13921 aatttttttt acaagcaaca gcggcataag cccgccccgc ccgccgtgca acagattgcc
    13981 tggattcccg cagagtcctc tgaactcgct gcggcggatt cagacgcaca ggaggcggat
    14041 tgccgcaagc ggtatcccga aggactcgat gccgttcgca ccgattttca acgcactcag
    14101 gcgttgctct ccctcagcct caccttttac gaattcgcct tggtcggtga tggcgacgac
    14161 aattcgttat acaacgcccg tgaactccac gacatccttg tggcattgaa cctctccgcc
    14221 gagccttccc tgggtgccgc ggcccatctg cgttcgctca ccgcgcactt cgattccctg
    14281 cacgaggcgc gtgggatgga agcgctcatg gccggcatgg gcaaactcta cgatcaaggc
    14341 tatcgcgtca ccaccgccga caaagcacac ttgaatcgag tgatggaatg atctccacgg
    14401 tgatgcgatg gcaggagctg gcacgcgtct ggattggcca ttggctcagt cgaccatttc
    14461 accatctgtt caatttgatc ccgggagtgg agatcggttt atcggcaaca gtcgtgaccc
    14521 ggcttccgct cacccatgcg ccgctggccg gacgacgcgc cgtccatttg agcgatctac
    14581 atctggaccg ttaccgcccc cggcaccgcg ccctgattcg agcggtggcg gacctgaagc
    14641 ctgattggat cttcattacc ggcgatctgg tcaacgtccg ggatggactt ccccatctgc
    14701 ttcgctttct cacggaactg cgccgcctgg cccccgtcta cgtcaccctc ggaaaccacg
    14761 atcactatag cggcatccgt gttgatgagt tcgccgagca attcgaccgc cgcaaggtca
    14821 cgttgctgct caaccaggtg acgttcattc gagtagcgac cggcgaactg gcgatcgtcg
    14881 ggctggatga cccgtcactc catcgcgcag accttcgctg catgccttcg gcacagcctg
    14941 ggcgtttcac cctggtgctg gcccacgcgc ccaacattct ggaccagctg cggcccgagc
    15001 accacaccga tctgacgctc tgcggccata gccatgccgg ccaatggcaa attcccggca
    15061 tcccgacatt ctggctaccg cccggttgtc atggacgaac tcacgggatg tatgaaaaag
    15121 gcggccaccg tttatacgtc aatcgcggac tgggttggtc ggtcctgccc atgcgcgtga
    15181 attgcacacc agaaattgtg ctcctcgagt gggccgcttg aacgactgac tgtccctctc
    15241 tatctcccta cccacattaa cgaatgcgct ccaacgcctc acgcgcccgg ctccgcacgg
    15301 tttgatccca atcttcctta ctgacatagg ccaacttgtc cttggctgcc gtcgcgcgca
    15361 ggcggccgag tcccaaggcg gcactcgatc gcacataggc atgatcgtct tccaacgcct
    15421 tcagaagatg cggcacgacc gtctcatccc cgatgacgcc cagatggctg gccgccatgc
    15481 cccgcacttg atgttgttta tcgcccagag cccgtttgag tgtcgtcaga aacaattcct
    15541 gaaagcgtgg cgcaatggcc tcaaggccca cgcgccggta ttcattcact tcaatcaccg
    15601 caaacgccag ctgcagtcgt ttgtcggccg cggtctggct ctcgaacaac ttgatcaacc
    15661 gagcgcgctg ctgatttcgc gtgcggcgtg cgcgaatctt tccgaccagc atccatacga
    15721 agaccagcac cgccagcacc aaggccgcca gcgggagcgc ctgatcgatg atcagatcct
    15781 gcatctccgg cgacaggcgc ttccacaccc agacgccgga cacgacaaga cccagcagga
    15841 gaaacacggc ggtcagattg gcttgtcctg attgtgagtg acgcggcatg gcaggcgaca
    15901 tgataggggg gcggtccttg cggcgtcaac gcaaggcctg tgaacgactc ttgacagctg
    15961 ccctcgcgga tgactagtct cgcccgcgat ggacctcccg gacctgaaag acgatcctca
    16021 tctcaaagtg ctccgccccc tggtcgagac ctatctcgca ttttggcgcg cggacagccg
    16081 ccatgtgcgt tcgcttcggc tcaccccgtc acaattcgac gtgatcgcga cgctcggtga
    16141 cacgaaggga ctcacctgcg cggaactctc cacggctacc ttggtgacca agggcacact
    16201 caccggtgta ctggatcggc tggaagccaa aggactgatt cgccgaacac cagtggctga
    16261 tgatcgccga agcacccgta tttgcctgac cgccaagggc aacaagctgt ttcagacgac
    16321 cttcgccgcc cacattgcgt tccttcgccc ctttttccaa cgcgcgctct ccgccgccga
    16381 cgccgatcag ttgcgcaccc tgctcctgcg tctccatcga agtttccagg agaaaccggt
    16441 cgcatgatcc gcctcacggt gctggggtca ggaaccaatg ttcaccccac acgcgcggcc
    16501 gccggatacc ttcttcaaac cgatcagaca ttcctgttgg atttcggacc gcgcacactc
    16561 tccaatttga taaagagcgg ggtcgatcgc cacagcatca cccatattct gttttcccat
    16621 ttccatgccg accacttcgc cgattttatt cccttcttct ttgatgctgt gatcttctgc
    16681 aaataccaag gcgggaccag accgcccctg acgctcatcg gtccacacgg caccgccacc
    16741 ttgatgcggt ccatgatgac gacgttcccg agtttcgatc gcccgccctt ccgagtcact
    16801 atccgcgaag tggccgatcg cagcatccgg atcggcgaga cgcgcatcac ccccgctacc
    16861 gtcactcacg cccccaaact gcattgcgta ggataccgtg tcgagtatca gggccatgcc
    16921 gtggcctatt cgggggattc tcaatattgc gagagcctgg tgcgactctg ccatgaggct
    16981 gatttcgccg tgctcgattg ctcgtttcct gaaaaccggc ccggcgccgg gcatttacac
    17041 gcaggccttt gcggccgcgt cgcccaggaa gccggcgtca accagctggt cctgtcacat
    17101 ttttatccga tcgccgaaca atatgatgtc cgcgcgcagg cgaaacacta ctttcctgga
    17161 cgagtcagaa tagcgcggga cctgctcaag ctgcggagct agtcgagaca cagcctctgc
    17221 acgatgggag aaacctgaag gggagaaacc cggcttacgt tgaggatctg aatgaggcga
    17281 gaacgacgct ggtggccgtg ttcagcttcc tgctacaacc cttcttcgcg ctgttcacag
    17341 cccaggattt ccacgaaccg tggtaagcgg tgctttttca gcggctcatc gagcttattg
    17401 tagatggcga ggagattttt gatcatccgg gccagaatag ctcgggtcgg gcttttctgc
    17461 aggaaacgat cttcaaagcc gtagccggct tcggtcagga accgcgcgca gttcttttcg
    17521 gtcaacagcg ccccgccgtt gaaacaatcg atgaaaattt tgtaccgatc cgaatcgaat
    17581 ttcaccagga agtggccggg catcccgatg ccatgcaccg gcaactgcaa ccgcctaccg
    17641 atcagcaggt agacggtcga gaggctgatc ggaatgccgg tccggcggtc catgacgcaa
    17701 ttcagatagc tgttttcgac ttcgtaataa tttttggtgt tccctttgaa cccggcctcg
    17761 gtgaagagat accgatttaa ggctctgacc gtctcttcgc cggatacacg ggacccgatc
    17821 tgctcccgga catcctgcgc catacgatcc aattgccgcc ggtaggcctg cacatccagc
    17881 gtgggatacg cataccgcgc gaggatgaag gcgccggttt ccagatcgac ctgatcatcc
    17941 ggaaaggcga tcagatcgcg gagctcttct tccaacctcg cgccgcgaat ttcctccagc
    18001 acggtgacga tacgatcggc catctccggc tgctcgattt ccgcctcctg aagcagcgga
    18061 acagcgcagt cgccgaaatc gatcagtttg ccgctgatcg ttttgacaat ccgttcatcc
    18121 tcgtcaccaa ggagccggat cagggcgcgg atttggcttt cgttcacgaa catggaagat
    18181 cctcgaggcg tcaccgtgct cacccgatcg cccgccggaa cgccttcgca ccaccataga
    18241 gacacagcgc atcgaacaac accatcacgc cgatgaccat tccgacatga ggcaacgcct
    18301 cggcccatcc ctgaagaatg agcggacgga cggcatcaat ggcccaggtc atcggattga
    18361 attgggcgag aaatcccatc cagcccggca tggccgccaa gggcacgagg gcggagctca
    18421 agaaaatcat gggcagcgaa aggaagccca gcacggaaaa gaagtcgccg tggctcttca
    18481 cggaaaacgc catggccatc gagatggccg ttaagccgac tccgaacatc atgccgatga
    18541 gcaaaatcgt cgctactccc gccaggccgg tcaccggctg cacgccgaat agccaggcca
    18601 ccgccaagat aacgaggacc tgtagcgacg tgatggtggt gacaaagatg aagcggctca
    18661 ggataacgga ggttcgatgg atcggcgtgg acatcaggcg ttcgagaaac ccgttttcct
    18721 tatcgaagag gaggtcgacg ccgccggcca agccgttatt gagcaccgtc atgaccacca
    18781 ctccggcagt cagaaaacta atgtaactcg acgattgagt cacctgcaga tccgccgcgc
    18841 gttgaaacag gttgccgaag aagatcagcc agaacaacat cggctgcacg agcgtaaaca
    18901 gcatgctgaa cttttcccgg ctcagccgcc gcacccagcg catcgtcaac gcccgaatct
    18961 cctgccaata ttctttcata tctcgatctg catgctcccc ggcgacagtt aggcgtcttc
    19021 ggccttcgga tggtcatcct tgatagatcg ccctgtatgg gcaataaaca cgtcatccaa
    19081 ccgaggccga tgataatcga tgaactccag ctggcacgtc aggcgattcg ccgcttcaag
    19141 aatagcgggc aacgctttct cgggcgaatc cacccgaatg tcgagcccgt ttgcggtcac
    19201 cgtgacggca cgaatggccg gaagatccct gatggcagcc gcgagcttct cgacgcgatc
    19261 atgttcacgc accgtcagcg acacgaggtc tccgcccaac ccgactttca gttcgttggg
    19321 agcgcccagc gctttaattc gacctccgtc gatgatggca atccggtcgc acagctgatc
    19381 ggcctcatcc aaatagttgg tggtcatgac caccgtaatc ccgcgctccc gcatggcgcg
    19441 cacatggtcc cagatgcgca aacggctttg cacgtccagc ccgagtgtcg gctcgtcgag
    19501 aaacagaatt ttgggatcgg gcaataaccc gcaggcaatg tccagcttgc gcttcatccc
    19561 accggaatag gtcttcgccg gacggtccgc ctgctgttcc agttccacca attttaacag
    19621 ctgggggatg cgtcctgcgg cgtcttcctg agagagatgg tagaggtcgg cgaggagttc
    19681 gaggtgttcc cggccggtca agaaccggtc gatggcccgt tcctgcggca catagccgat
    19741 tgtctgccgg acacggtccg catccttcac cgaatcgagg ccgaaaattt tggccgatcc
    19801 ggacgtggga ttgagcaggg tgatcagaat tcgcagcgtg gtgctcttgc ccgcgccgtt
    19861 cggaccgagc agaccgaaga tctctccggt gtaaacttga aacgagaggt cctcgacagc
    19921 ccgaaccttg tcgtaggtct tcccgaggtg ggatacgtca atagccaccg gccgcgacat
    19981 cagccccatc ctccggcacc gcgccgctcc gccagcttga attgaatcaa atcgctcaga
    20041 cggcgggcct gttgcggcac atgctctgct tccagcagga actgtttttc caacggtgta
    20101 cagtcgagat aggtagagag cgaattcacg aacacctcgt cgctcacgtc gggtcggatc
    20161 aagctgtgca gcggggaggc ctcctcatcg gctttcagat attcatcaag cacccccatc
    20221 aggtaccgtc gcagagcttg gtccagggat aacgacccgt cgcgcggctt caacgtgacg
    20281 cgggcttcac gatagctttt ctcgaagaac tcttcatgaa tttcgtagcg ctccaggcct
    20341 tgcagcagga tgttggaccg gccatcgggg agcatttgaa cactcgccaa tcgcccgaca
    20401 cagccgatgg agaaaatggg aggattccct tcgtactgtt cctcccaacc ttcctttaat
    20461 aaggccatgc cgatacattg ccctgccgcc gccgcatccg ctaccatctg gcggtatcgc
    20521 ggctcgaata catggagcgg gagataggtc ttcggaaaga aaacgacgtt aggcagaggg
    20581 aacaagggaa tgcgttccgg gacaggaaac ggctggacct ttcccgacaa ctcccggttc
    20641 gacggctcac gttcaggctc aggaaacatg gctcacgata gctgatgctt tgcaaaattg
    20701 tcaacgctgc caccgggccg catctcgctc tcaaacatca caaaacatgc atggatactg
    20761 gcgttttatc gactgtccat caaaaaagac agtgggccca ggacagacct gaagcactga
    20821 cctgcacgac tggtcgctga tgtatctcat gacaccatta cgtctcggtt gcacgattcg
    20881 ccttctactc ctggcagttc tccccgtcac tgcacaggcc gtggcgacga atgagacggt
    20941 tcacgccttc gacatggcga caccggggta tcagtatcga tttccacacg accacggcgc
    21001 gcacgaacga tttcggactg aatggtggta ttacactggc cacgtcgtgg cggctgatgg
    21061 ccatcgattc gggtttgaac tcaccttctt tcgccgaggc atgcctccag accaggtgca
    21121 gacgcttccg tccagatggt ccattcagca gctctacctg gcccatctcg ctctgaccga
    21181 cctggatgcc ggccgatttt attatgccga caaagtcagt cgcgaaggcc tcggcaaggc
    21241 cggcgcggac acagaccggt tacacgtgtg ggtggaccgc tggtccctgc tcaccaatga
    21301 tccagcagca tcgcgccacc agcttcaggc ggcgaccgac agtgtggcga tcaatctcac
    21361 ggtcatcccg aaaaaaccgc cggtcataca tgggcacgat ggcctcagcc gaaaaggcgc
    21421 cgatccggga caggcctcgc actattattc gctcactcac ctcgcaaccg aagggcagat
    21481 tcgagtcggc accgaaacct ttgccgtcac gggcctcagc tggatggatc atgaattcgg
    21541 ctccgccgat ctgggactga atgtggtggg atgggattgg ttcagccttc agttgagcga
    21601 ttccaccgaa ctgatgtggt attccttgcg acgcgccgac ggatcgagcg atccggtctc
    21661 gagtggcacg ctggtcctgg ccgacggacg cgcgcacgca ctcgacgcgc aggacctcaa
    21721 catcgaacca ctggcccact ggaccagtcc gcgatccaac ggacggtacc cgcagcgatg
    21781 gcgactcacc tcggctacga tgggattgga tgtgactgtg gagtcgctcc tcgcggacca
    21841 ggaattggat acggctcgca gcacaggcgt gacctattgg gaaggggcgg tgtcggtctc
    21901 cggtcaggtg cgaggcgcac cggtcacagg acgcggctat gtcgagatga ccggctatgc
    21961 tgaacgtttc acgccaaagc tctaacgctt caggccttcg ggacgatgtc tcaccttgac
    22021 tcagtcacga cgctgtggtt aggtctgcga ccactgtgat gtcccaacga attcgaatag
    22081 cgaccctgct tcggcggtcg atttgcctcc tcagctttct gcttctggcc tgcggcagtc
    22141 gtgaatcggc cgtggtgtcg atcgcgctcc atccgtcaaa ccccaatatt gtctatgtcg
    22201 ccacgaacga tgccgtgcac aagacccggg acggcggagc gacctgggaa cagtttccag
    22261 cgttcaccgc ccgccgcgtc acgacgctgg ccatagatcc caagcttccc gccaccgtgt
    22321 acgccgggac catgggcgac gccatttata agagtccgga cggcggccag cattggcttc
    22381 cccacaacgt cgggctcaaa gagcatgtgt cgttcatcaa tgaaatcatc ttccatcccg
    22441 acaacacgga actcatttac ctcgccacca cggtcggcgc cttcatctcc aaaaacggcg
    22501 ggcgcgaatg ggaagaacgg atggccggga tgaaggaagt gcacatcgtg acgtgcatta
    22561 ccatggacca tcaacacccc cgggtcttgt acgcgggaac gacgggcggc acctatcgca
    22621 gtgaagacgg tggtacaagc tggcaaaaaa agaataccgg gctcattccc gaggatgtgc
    22681 tgaacgcagc catggcgttg ggcgtgaata tgctggcggt tgatccgcgg aactccgaag
    22741 tggtctacgc cgcgaccacc aaggggctgt ttcgatcagc gaatcgcgga gagtcttggg
    22801 aacagatcgg ccaaggattg tcgaaccaat tcatcagtac gctggcgctg catcccatgg
    22861 atgcaaatat tttctacatc ggtggcccgg acggtgtgcg aaaaaccacc gatggcggaa
    22921 aaacctggca atcacagaat gagggactca ccacgacgaa tatccgcacg atcagcctca
    22981 gcccgagtga tccccagcta ttgtatctcg gcaccaatgg cagcgggctc taccgctcga
    23041 ccgacggggg acaacattgg gcgccgattc ccctcacggg accacagcaa cccccacccg
    23101 cgtcataacg aggctcgctg gactaccgat gccgacactc ctgttgatcc gacatgggga
    23161 aaccgactgg aatcgatcgg gacaagtcat gggaaaccag ccgattcctc tgaacgagac
    23221 cggcgaacaa caagccctcg cgtgcgcgga gatgctggcg cagacaccca ttgcaggcat
    23281 cgtgaccagc cctgtgctac gggcggtaca aaccgcccac atcgtcggac gtcgccatgc
    23341 agcgcccgtg catcatgccc cgggactgag agagatcggc gtgggaaact ggatcaatcg
    23401 ctattggaag gatttggcgg atgatcccgc caagcgcgac tggtatctgc acccggaccg
    23461 ggcccgccct tcaggcggtg aaacgctctg cgaagtgcaa cagcgtgccg tcgcagcagt
    23521 cgaacaggcg ctgcaagtgg cgcaagatgg accctacgtg tttgtgtccc atggcgacgt
    23581 cattcgggcg gtgctggccc attatctccg gctcgacttg aatgcgctgc gccaggcgcg
    23641 catcgaccat gcaggggtca gcggcatcga ggtgactgaa gccgccaccc acctgctgtt
    23701 cctcaatcat cgcccaggcc tccccaggct ggcctgaacc acactccacg ccgttactgg
    23761 tgcgcagatg ctccctccgc gccttttccg tagtagcggc tgccataccg cttcagttct
    23821 tcggacaagg cggtccattc ctcagccgtc aacaaggctt tcatcttgaa ccgaaggccc
    23881 aagatctttg ccgaggaacg catccggctg ttattcaatt cgtcgaggat tttggtgaac
    23941 tcctcgggcg cagctgaata gtttgcgttc aactcataga gcgcgcgatg aaactggcga
    24001 tgttgttccc gagccgactt gatctcggcg atcatctcgc ccatcaccac gttcacctgc
    24061 ttgacggttt ccggattctt gacgtgtttt tccaccaggc cgctcacatc ctgagaggcc
    24121 ttgccccaat aatagtcgcc cttggcatgc tcataacttc catggtggtg ggaacaaccg
    24181 gtcagtccgg tcaacagcac accgacaaga agacacaggg atcgaacgct catcacaacc
    24241 tccattgaaa aaaatgactg tgtcgcatga aaacgcgttg cgccgcagcg ccttcctccc
    24301 gtaagctttc cgaacacctg acagggcaag gagtacgggt atgggcgaac aaaaggcccc
    24361 tcaacgcgga acagggccgg tcgaatcact ccgggacgaa ttccgtcaac acctcgacgt
    24421 cttttacagc cgcctcaaac tggccgctcc ctacgacagc gtcgaaaagg ccttgggcac
    24481 gctcaccacc acgctcaaag cgttaccgcc tgaggaactg caacgtctgg gcgcagacca
    24541 gacccagcga tgggctcact ttcggcaggc cttcgtcgat tcgggcctga tccagaaaca
    24601 ccggggaatt attgcaggcc tggcacgatc cagcgcctca ccggatctgc cgcccgaatt
    24661 caatcatctg ctcgatctgt atcgccccaa aacttagctg acaggttcac gaaggcgtgc
    24721 tggattcagg cgatcttttg gcaaatcagg ggcgcgatgc tgccccttca ttggccaacg
    24781 cgatgtctct cagttccgtg tacacgagca tcagccgcgt gttttcgatg cgataggcta
    24841 ccgtgaccgt cagcaaccca aaggcgacga cgatcaatcc aactccggtc acgccaatcc
    24901 ccagcagcac acttcccaat gactcgagcc cgccctgcaa cacgtaggtg atgaggaacc
    24961 cgagcgttcc gaatcccacg agactgaacc agaggaacgt caagatcgag gacgaccagg
    25021 gtacccgctt cgtacacacc atgcgcaggc cgtcagagtc cgatgccaat tcgatgctgc
    25081 ccttgagcgg ccaggccgtg cggaagcgca tcgagaacag gcgatgcgtg gggcggatga
    25141 tcattcgatt cgcgtcggga aacacccgcg cgaccccgtg aggcaagctc aaaaacccat
    25201 tcgtgtcgaa cgcgcgcagg agctttggaa ccgtgagggc cgcaaaccgg tcctgcactt
    25261 gcccgatgcc atagccgtac cgggtggcat cggacgagag gcgggtaaag tacatccagt
    25321 cgccgacgat caatccgata aacaacacga tggcaaacgc accggcgagc atcatagccg
    25381 aaacttccca cagggcttta cggagaatca agtccgaacc gatggtgacg acaggcaaaa
    25441 agcaggagga acctgttgac tacccatctg cgcattggct acattaagcc cgttcttatc
    25501 cggcgggtga ggacgagtca tccgagttgc gttcccgcct tcatagattt ttccacgcca
    25561 cacttcttat cgccgtcact tctgcactgc agagttgtgc gggcagagcg gcgtgatttt
    25621 gcggagggac gatggatatc gccaaagtcg agcgtgtgta caccagttat tccggcgtct
    25681 atgatcatat cttcggaaaa atctttcatg agtcgcgcga agcctgcgtg cgcaacctcc
    25741 gaatccggcc ggaagaaaac atcctcgaag tcggcgtggg aacgggaatc gccctcgaat
    25801 attatcccaa gaactgcaac attacgggta tcgatctctc ctcaggcatg ctggccaagg
    25861 ccagacaacg ccagtcgcac tatcatttgg accatgtacg gctgatgttg atggacgccg
    25921 ggaaaatgga attccccgac gatagctttg acacagtgat ggcggcgtac gtcgtcaccg
    25981 ccgtgcccga ctatcgcaaa gtcgtcaatg aaatgatccg cgtgtgcaag ccgggcgggc
    26041 gcatcatcat gctgaatcac ttcagcaacg gcaataaggt gatcgccgcc gtggagaaag
    26101 tcatttcccc tctctgcaag cacatcggtt tccgtaccga tctctcgctg aacacggtgc
    26161 tcgaaggcac cgacctccac gtggcccgaa aagaaaaagt gaatccgatg aagttctggc
    26221 acttggtgga atgtgtcaac cggaagggta ccaagagcag caatgggaac ggcagcgtga
    26281 acggaaatgg caacgggcgg cacgggcacg tcagttagag tgcttggtcc gttgagattt
    26341 ggatccggcc tatgggccaa atcctcactc gtcttttctc accactcgct tgaaacgccc
    26401 ctctcgccag tcaggcaggt acccacatac cgctgagtga ccacttcagt caccgccctc
    26461 aacaaccggc cgctgaagcg gccagtggcg agtcgaggct gcagtctgat tccgaaccct
    26521 gctccgtcga tcggttcata ggcccgcacg gtcagcgctg ccgtggggcc ggcattcacg
    26581 tcggcggtag acaccaactc cccttcccaa cacaaagtcc cgtgatggcc cgcacgccgc
    26641 ggcgtcgatt tccatatgca agggacccct acctgatcaa gttgggtcag cagccaatcc
    26701 accgtgggag ggtcaccgga atcagcctga aagatccagg gcgcggctcc ctggtgaatg
    26761 tccccgtcgc tatgcggtac tgacacctcc tccaaaagcg cctcttcaca caccacgtac
    26821 aggttcccct gcggatcgaa ccagaagcgc aggaggcctt catgcaactc cacctgaatc
    26881 attcccagag cagcacagtc cccgccgtcc tgctcaaaga cggaaaagtc ggagacaccg
    26941 tgcagactga gtttggcaat cctgaggacg ccttgcagat gatatcgtac ggtgactgtc
    27001 acgactctcc ctgccgacac gtcccggcct gactcttcgt cgagcatcca gcgtttggcc
    27061 aaatgcacat ccatcacacg accgccgcga aacccctgcg tgtgcgtcag caaccaacga
    27121 acgtcgtcgg ccgttttaat cgaatgtgtc atcgcaacgt gaaaaccttc aagcaagatg
    27181 ccgtctatcg acaccctctc gaaccactgt atcacgcgga cgccggagaa aagcgaggga
    27241 aacgacggtt ccaggagagg aggtaacggg gaaagggatg ctagaagagc acttgctgct
    27301 gcttggcttc tgccagttga cgctccaatt cctctacacg ttcctgtaga cgctgattat
    27361 caaggcggag aagatcgaga cgatccagca attgttcgcg ctgaatgttg gtccatttca
    27421 gtgtgcgctg caagccagcc agttcaagct gtgcctgctg cccgtcttca aacgcggagc
    27481 ccatgcgtac gcctcagtcg atcgccatca ggagtgatag gcaaatcgta gcgcactctt
    27541 agccagttgc ggagcgatta gtgatttatc gacgagggac gcgcgggaac tgctctcagc
    27601 tgcgtgggtg agacgaaact ctctcgaagg gcttattttt cgcgagctcg caagggcaag
    27661 gtgctcccct cgaggtcgtc gctggactcc cagcgataac gaaccagcac gccgttagcc
    27721 tcccacccgg gtgggcagag gttgcccgtg ccataatagt cggcatcgag cactggaaac
    27781 ggcacgacat tcttgatcca gtcctgagca tggccgttag actcgatata ggactgcaaa
    27841 aaatacgcgc cgccggtcaa aggcaggtcg ggcagaatga aatccacata cccggttcca
    27901 cgtagggtca aaggcgtcgg atcaacgata tcggtcgaca agacgaatag atcctgtccc
    27961 ttccgcccat tgacggaaac gctgacccgg cagttgcgaa attctttacc ctccgcgcag
    28021 cggtagtgca tccggatgat cacatcccgt cctgttatgg cagaagtcac ggagtgccga
    28081 tccttgtcca gtagatcgat ctcagtgagc aggatctctc ccaggccgac tcggtccggg
    28141 cgatcgccga tcgacatgct cgcctggctg cgaatcgcat cggcatatcc ttcgactacc
    28201 gtctgcgttt tgccgattct tgcgatgcgc ccattttcta gcagaatcgc ccgttcgcaa
    28261 agcccttcga tgatacccat attgtgactg acgatgagga ccgtgcgccc gcttcggctc
    28321 acttcttcca ttttccccag acacttttgc tgaaaactcg catcgccgac cgctaacact
    28381 tcgtccacaa tcaagatttc cggttcgaga tgcgcggcga cggcaaaggc caatcgtaca
    28441 tacatgccgc tcgagtaccg cttcaccggt gtatcaatga aatcttccac accggagaag
    28501 gcgacgatct catcaaactt tctccggact tcgtcccgtc gcatgcccaa catggccgcg
    28561 ttcagaaaaa tattctcccg gccggtcaac tctgagtgaa agcccgttcc gacctccaag
    28621 agcgagctga cgcgcccgta gatatccacg gtgcctttag tgggctgcgt gatgcgagac
    28681 aagatcttga gtaaggtgct ttttccagcc ccgttgtgcc cgatgatgcc cagcacttcc
    28741 ccctgcttca cttcgaacga aacgtccttc actgcccaaa acgagggatc gggttcagac
    28801 cggccggagc cccatcgagt gagggacttg agccggtgca tgagctgatc acgcagcgtg
    28861 ttgtgctgga cagccgcccc cagtcggtaa tgcttggata gcccttctgc tcgaattgcg
    28921 acggtactca tctaaatcac gtccgcaaag gtgcgttcca tcgaacggaa atagatcaat
    28981 ccgccaatga acacgacaaa aacaatgccc agactgggaa gaaccatcga aaagtcgacc
    29041 tgaaagcccg gaatcaatgc ccaccggaat ccttccacca cactcaccac cgggttgagt
    29101 gcatagatgg cgcgccacga ttccggcacc ttactcacag ggtacagaat gggcgacacg
    29161 aacatcccca actgaatgaa catcggaaga atatgaccga catcccgata tctgacgtag
    29221 agcgccgaaa cccagagccc tgcggctaat gtgatcatcg cccccaatcc ggcgaacagc
    29281 ggcaggaaca gaattctcca ggtcggcatc acttcgaacc aggccatgag accgagaaac
    29341 gcaatgaatg ccagaaataa atcaaccagc ggcgcaatcg tcgcggagag gggaagaata
    29401 agccgcggga aatacacctt actgatgagt ggcgcctctc ccaccaagct ggtcccaccc
    29461 cgactcaatg cctgagagat ataaaaccat ggcagcagtc ccgcgtagag aaaaacagga
    29521 tagggaaaac cgtcggaggg aatcttcccc atcacgctga acacgaccgt gaaaatcagc
    29581 atggtcgcca caggctggaa catcgcccag gccacgccta acagcgtctg cttgtaccgc
    29641 acggtcacat cacgccacac cagaatatgg atgagctcgc gatactgcca aagggcagcc
    29701 aaatccaatt ccagcaaacc cttggccggc tgaatgcgca catgcggcag caccgccgga
    29761 tgtcgctggt cgatgtttac aacagacatt tcgtttgagt ggttcctata ggcctagaca
    29821 tgtccagctg gaggacgacg cgcagagacc gaaatcctag tgcagccgca caattgatcc
    29881 taactcgtac tgaataccat ttgttatgtc aaattcatga cgctcggtca agctcgatac
    29941 attgccattg gaacaacatt tctctcttga ccctatctct gaacaacagc tcttccgaat
    30001 ggatgcctga gcgatcctgc tgccggtaaa aacgcggaag ccacaggctc gttgacttct
    30061 ccagcctcaa cgggacaatc gatacacgga gtgacgaagg accgtaacag tcgcttcagc
    30121 cggcagggag cccggctatg gcgtggagag gggaggcttt tgtgcggctc gcacttcttc
    30181 tgctgagaga taccgaatcg ctaacccaga catgtggagc atccaacctt gcccgccgcc
    30241 agccccttga gtaaagctcc acgcgtccat ccggaacggg ttccaatacc cgctgcggta
    30301 agaattggta ttcgataccc cgcccggtac ggctgtgccc atcgttgatt gaaacgtcgg
    30361 gcctctatcc gacagacgcc ggacatccgc gtctcctaat acgaccgcgt cagcccccaa
    30421 ttctttggct cgctcgagaa tgcggtcacg caaggtatct tcactggcat attcactcgt
    30481 agcgacaatg cgggcgattt gcacgtggcc gtggttaggc tcgcgttgta acgtcgtcac
    30541 agcatccgcg cccgatcgag gttcaaacgc cttggtggtc aacggatcca cctggacaga
    30601 gacacaggcg ctcgaaaggg ctagggagag cacggctgtg aggtggatcg ctcgtctgat
    30661 cacaacaacg cctcgtgtgt ttaggaatta ccgcccgccc gtgatgttca tgtcgccttg
    30721 gctaccatgc atcttgacga ctcaggtcaa tcaaatcaac cacaaatcgc ctgccctcgc
    30781 agcttctccg gggtaatcct gcatcacgtg gggtaggctg tccaatttcg tactacaatt
    30841 tcccttcaac gagggtgcga atctcttcca tgcgccgccg attcacacca aggtcgctgt
    30901 agccggtcct ggaagccgag cggaattgaa tcatcttcgt tgcgtcatca aaaacaaatt
    30961 ccacatcatc gatgaaccgc agcaagaagc tggtgaattc atagtgtaga tacccgtcgt
    31021 tttcttcgac gagtcgggtg cgaggcaatc gattgatgat ggtcttcagc gtgttttgcg
    31081 cgccagccag attttgctga taccgatatg gcgcgatctg gcgactctga tccgccgctt
    31141 gcgtcgacac gcagttggga ctcgaggggc agggcgcaag tattttcatg gcatcgtggt
    31201 taggatggtt agaacgtaca ccgaactcta ataagccgcg cctggtctgt caaccgttcg
    31261 cccattgcaa accacgtctc accggcggcc cgacggcacc gcgctggcac ttgtgtccgc
    31321 tcggcgagcg actagccggg cgtgccaacg tggcacggcc tccatgccgg gcttttccac
    31381 caggcggaac gtcgcagaac agaggagaat cagcgcagga acctgacaca gcaccatggc
    31441 ccaatgctcg gcgggggata gcgtggcggc cgactcggcc agcaagagcc ggtaggttac
    31501 aaacaacacc acactatgca ggagatacaa gctgtacgcc atttcgccca ggacaatcga
    31561 cgccggccat tcgagaatgc cgtaaagaga attcccgcag gcaataatga gaaacccacc
    31621 ggccaaaagc agcgtggccg gaaccgtata cgccgtcgga aacgccataa acgcggtcgc
    31681 cagacacaag atcgcgatgg cgccccacac accacggctc agcatcattc ggatggcagc
    31741 aatacgcaca agccctgccg ccgcaattcc accggcaaac gcatacaata ttgggaaccg
    31801 aacctgtggc atcaccatca caagaatcac cacagccgcc agactgacga tcacccactg
    31861 aatcggaggg cttgtccgaa gacagaaggc agcgagaggc aaaccggcat agaacaacca
    31921 ttcatatggc agcgaccaca cggccccggc caagggcacc gtttccgcaa atccattgac
    31981 ggacgagata ccgggaattg taaacagcag ccactgtgcc gtctgttcgg ccacaactgc
    32041 cggcgcctca cgcatttgaa aacccgtgcg gccaaacgac atgagcaata cacaggcgac
    32101 cgccacgaga tacaacggtc ctaggcgtag cgcccgagac agatagaggc gcgaccagtc
    32161 gatcggttgg tgacgtccat cgaggagctt tgaccagaag agaaacccgg tgatcatgaa
    32221 gaagagggtg acgctgcctt gtcccagctg cgtgtacaca ttggaggggg gaacgtccca
    32281 ggtgcccgat cgcaaataga agtaccagat ggaggaatgg ctgacgaaca ccgccaacgc
    32341 cagataccct cgaagcccat cgagcgacgg atagcgcggc gttgacgagg cctcctgaag
    32401 agtagttcga ggaagaaaca tggccgtggc gagcgcgaca agcaccacac ccgctgcggg
    32461 ccataaactt gttgcatcca tggactcacg acatcatagg acggcacatt tgtctaacct
    32521 ttctgagatc ggcgccaacc cgtcgcacgg cgatgctgac gttcacgacc gctcccccat
    32581 cagccgctgg gctggagcgc cactccgaca ctcactccgt tgattgcgag agtggtcctg
    32641 gggtacgcta gcccttccga tttccgattg acggctgcca gccgccacct atcgtacact
    32701 gctgagcgtc accgctcagg ttcaacaaag gagtgctctc gcatggccgg ttttccgatt
    32761 gaagtcgcca cccgcattaa aacgctgccg ccctatcttt ttgccgccat cgacaagatg
    32821 aaacaagagg cgcttgcgcg cggagtcgat atcatcaacc ttggtatcgg ggacccagat
    32881 ctgccgacac cggcgcccat cattgagagc ctctatgaag cggccaagaa tccgaaacac
    32941 catcaatacc cttcctacga gggcatgctg tcgttccgca aggccgtagc tgactggtac
    33001 aagcgacggt tcaatgtcac cctcgatccg gcaaacgagg tgctgaccct gatcggctcc
    33061 aaagaaggga tcgggcacgt tcatctcgcc ttcatcgacc cgggcgacat tgtcctagtc
    33121 cccagccccg gctatccggt ctatccggtc ggaaccagct tctgcggcgg cgtctcgcac
    33181 atcatgcccc tcacgaaagc caacggcttc ctgccagatc tcaatgccat cccgaaggat
    33241 gtggcgaagc aggccaaatt gatgtggctg aactccccca acaatccgac gtccgtcatc
    33301 atgagcaaag actatttcaa gcgtgtcgtc gagtttgcgc aggagaatca gatcatcgtg
    33361 tgccatgatg cggcctattc ggaaattttc tatgacggca aacggccggc cagcttcctc
    33421 gaagtcgatg gcgcgaaaga ggtcggcgtc gaatttcatt ctctttccaa gacctacaat
    33481 atgaccgggt ggcgcattgg attcgccgtc ggcaacaaag aggtcctggc agggctcggc
    33541 agggtcaaga gccagctgga ctcgggcgtg ttcgaagccg tgcaagcggc cggcattacg
    33601 gccctcgggc tcgatgattc cgtgacagat ggattacgca aaatctatca agagcggcgc
    33661 gacaccctcg tcccgggcct gaaaaaactc gggctggagg tcgatgcgcc tcccgccgcc
    33721 ttctacatct gggtcacggt gccgaaggga tatacgtcgg catccttcac cgctcatctg
    33781 ctggaaaaag ccggcattgt cacgacaccg gggaacggct tcggcgcgcc tggtgaaggc
    33841 tatatccgca tgaccgtgtg cacaacaaag gagcgtcttg cggaagcggt cgaacgaatt
    33901 cgcaaggtgg gattctagcg tggcggctat tgcggtgcct cctgtctccg caggtggctg
    33961 acgcagtatt cagcgtgctg cgtgcaggac gctgagccgg cttcagacta tggcaacagc
    34021 atttatcggg ttcggctcta acctgggcaa tcggatcgat ttctgtgatc gggcggtgac
    34081 cctgcttggc ctcctaccgc attcacggct ggatgcggtc tcgtcgctct atgagaccga
    34141 accgattgag gacggagcgg cccccggccc cgcatggttt ctgaacggcg ccgtgcaagt
    34201 cgaaacgaac atcacgccca agagcctgct ggaaatctgt cgcgaaatcg aacgggcact
    34261 cggacgagat caagacaatc gcaaggggcc gcgcacgctc gatctggatc tcctgctgta
    34321 cgatgactgt gtgctgaatg atcccggcct catcgttccc catcctcgtc tccatcagcg
    34381 tcgattcgtt ctcgcgcccc tggttgagct ggatcccgat cgacgccatc cggtgctcgg
    34441 gcaaactctc cgagaacttc tggaaacact cgccgatcct tccattgtcc gccggctcga
    34501 tccccagccg aactcacgtt acggatcgag gccggcatgc agtccacccc ctgtgcagag
    34561 tcctcacacg tgaagatcat ccgcacgact tcagccatgg ccaactggag ccgtggcctc
    34621 catcgagaag gagtgacgat cgggtttgtc ccgaccatgg gggccttgca tgagggacat
    34681 cgcgcgttgg ttcgagccgc ccggttggcc tgcgatgccg tggtcgtcag catttttgtc
    34741 aatccgaccc agtttggtcc gacagaagac ctggcgaaat atccacgcca atttcggctc
    34801 gaccgtgcac tgtgcgcgaa agaaggagtc gacgcgatct ttgccccgac cgccacggct
    34861 atgtacccgg ccggttttca gagcattgtc gcggttccga gcctggcacg gcgctgggaa
    34921 ggcgaggcgc gcccgcacca ttttcagggc gtggccacgg tggtgacgaa actcctgtcg
    34981 ctcgtccaac ccgacatcag ctggttcgga cagaaagact ttcagcaagc cgcactggtc
    35041 cggcaactgg caaaggacct caatctttca ggcacattga gggttcatcc gacgatccgt
    35101 gaacatgacg ggttggcgct cagttcacgg aacgtgtacc tctctgcaag ccaacgtgac
    35161 gccgcacctg tgctctatcg ggcactgcag gccggagctg ctgctattcg tagcggcgaa
    35221 cggcgagggg cactcataca acgcctcatg actcaaatga tcaagacaga gcgatcagtc
    35281 acaccggaat atctcgccgt ctgtgatcct tctactctgg aacctttacc gacaatccag
    35341 cgccacgcca ttcttctcgg cgcgattcgc ctcggaaccg tcagactcct ggacaatatg
    35401 ccggtggcga tcagacgcaa gccctgaaca ggaaggcgat ggaatctgcc cggcctcgca
    35461 tgaggacagg cctatccatc cataaaaagc gatccggctc tgtctgaatt atacggagat
    35521 gcggggaggt gcttgttggt acgcgaggag ctcgagaacc aattgcccca tgcgctgttt
    35581 atcttcaggt tggaattcta aaaatttcat gccgatcgac tgaggctgaa tcgagcagat
    35641 catcgccgcc tcgactttga tatccggttg cccggatggc ccatgcagca cgaacgtcac
    35701 gaaggtcccg cgtggaagcg gtgtggtcgc ctgcaacgta cacccaccca tggaaatatc
    35761 ggtgaccacc cctccggccg ccgtgtgatc gagcctcagc gtccccgcaa agttgacggg
    35821 caaccgacga tactcacggc gatcgaatgc ttgcgtggtc aaccggttac ccggccggcg
    35881 agcctgaaag cgagtcgtgc agagctggca ccgaacctga aaaatggaca gccggttcaa
    35941 cgtcttctct ataagacttg tcccggacgt cactctgacg cacggcgtac cgcaagccgg
    36001 acaatacaac tctttcatac tacgcttctg actcctgact ccttgtctcc agatgcgagg
    36061 cggagcccac gcttcccgac aactgttttg caagcagtgg tttgacatcc gcgggagagt
    36121 aggtcggagg tttcacccac tttccatcct cccgcttgta gccgcccact ttgctcaaat
    36181 tcgatcgatg cacctcacgg aacaccggat ccatatctac gccgtaggac accgccgtgc
    36241 cgtacaccac atacaacaga tcagccagtt ctttcgccac cgccggaaga tcctgcgcag
    36301 tcatggcctc ctgcagttcg gcaaactcct cttgaatcag ccgcacccgc agttgccggg
    36361 tggcgtcagg aggaatcgac ggccgctcgc cgatggggat atcgaacttg cggtgaaact
    36421 cggccaccat gtcctggggc tgcatcattc ttacgttctc atatcctcgg ccgcaaaggg
    36481 aaatcctgtc ctcggctcga taccggtgga ttccagcatc gcaaggtctt tctcagatga
    36541 tatcccgaga tctttaaatt ttctagcggc aggcaacacc cgggtttcca aggaggccac
    36601 cgccttgtta taggctccga cgcttttccc taaggcctga ccgatatcat tgagatggtc
    36661 ggtgagcacg gccatccgct cgtagagatc cttgcccagc cggcctgcct gttcggcatg
    36721 ttcggttaac tgttcttgcc tccatccgta ggcgaccgcg cgcaacagcg ccatcaacgt
    36781 agtaggcgtg gccagaacaa tcccttgggc aaacccatcc tcgatgagag aaggatcctg
    36841 ctccagcgca gcgccgagga actgctcacc gggcagaaac agtacgacga attccggtgt
    36901 ctgtccgaac tggttccaat aggctttcag gctcagcgca tccatcctgg ttcgcacctg
    36961 gttcgcatgg cgacgtaaat gcgcctgccg ctgctggtca tcctgggctt cgtacgcatc
    37021 gagataggcg gccaacaccg tcttggcatc caccacgatc tgtcggcctg caggcagata
    37081 caccaccatg tcgggacgga gcagtccgtc cattgagtca accgagtcct gttccagaaa
    37141 atcgcaatgg gccaccatcc ccgccagctc agccacgcga cggagggtca tttcccccca
    37201 ccgcccgcgc accgccggag cccgtaaggc tttgacgaga ttgctggttt cctgctgaag
    37261 ccgctgatgg gattcggcca tgaacttcag ctgctggtcc agtccaccgt attcccgttg
    37321 gcgaatctgt tcggcctgtt gtaattgttc ctgataacgg tggagcgatt cttccagcgg
    37381 cttcaccagc tcaccgatcg cttggtggcg ctgagataac tcccctttgg cttccgcgtg
    37441 caaggtttca aatgaggcgc gagcgaggtt gaggaacgcg gcattgtttt gtttcagggc
    37501 ttgcccggac aaggcctcga aggattcctg cagctcctgc cgcgcctggc ttaacatcgc
    37561 cttttggtct tccatgctcc gactgatgac ttccatccgc gtttcggctt tcgtccggcc
    37621 ttcttgagat tccgccagcg cctgccgcaa ctggtcaact tcggactgtt gagtgtcgag
    37681 ccgctgttgc aatgccactg ccaaggcatc agccctttgt gcacgctccc cgctttcgag
    37741 caactgtgcc tggattctgg attgcgtgcg agctgtcacc caccatccag tgaccaggac
    37801 accaatcccg agtccggcca cgcctcctgc gataaaggtt gtcgaggtga gatcgatcat
    37861 gatgcatcct gataaagaca gagcatcgcc gccatgcctc gcacctgccc gttgtaggga
    37921 aatcgcgtag agggtacgag aaaccagatc aaacgtcaag ggaccaagcg gctgatggtg
    37981 cctgtcgaac tcacaatgtg aaccggctcg tcctcacgca agagggtggt agcatagggc
    38041 acattgacca ggaccggaag gccgtactcc cgcgcgatga tggctccatg cgacagcgtg
    38101 ccgcccattt cagcgatgag gccggccgcg ataccaaaaa gaggcgccat ccccggatcg
    38161 atgaccggaa cgacgagaat gtcgcccggc cgtactcgct gccaatcctg tgtggaacgg
    38221 acgatgcgga ccggaccact cgtggttccg gcactgacgg caatgccccg cagcagtccc
    38281 ccatcgactt ggccggtcat ggacggtccc tgctcgagaa ccgcttccca atctcgaacg
    38341 gtgtcaggca cagacgcatt ttggtcacgc gttctctgct ctcggcgtgt acgtacgagc
    38401 cgttgccaat cccgtgatgt cccctcattc agtgccaccc gctcatccat ggtgagataa
    38461 aacatgtcgt caggctgagt cagaattccc tgctccacca ttcgctcgcc gagttgaagg
    38521 aggagatggc gcacggccgt ggagtagtag agtaaatgat ggcgattcgc ctcacgcagc
    38581 gcgcaaaaac ggctgagccg ccgataccac cagcgaaaaa ccacccctcg gtgacgccgc
    38641 cagccgaacc gccggtaaat ctccgcaagc gccgcttcgc gccgctgtgc ctgctgagtc
    38701 atgacatcgc ccggcggaac ggccttcccc tcacgcaact gggtccgcaa caacatcaag
    38761 accgaatcgg gctgatccgc gatgcgcggc gacatgatgt ccgattctcc caccgcgcgg
    38821 tgtccgtaat ccgccagata ctcatcaaac cgtcgcagaa agccggtgcc ggcgagggcc
    38881 tcgcgaaacc ctgaggccgc ccagccttct ctcagaaacc accgctgaac cacgctctca
    38941 cgacttgcga cttcgactaa ttccgccaga cgcaaaatgt gttgcgcact gatcacgctt
    39001 ccctggcctt gcaaagaggc gttcagcaat tctcgccagt cttctcccag ccacccgggc
    39061 aagaaggtac ccattgcctg cagactctgt gcgacccctc cagcgattcc gaacgtgacc
    39121 tcgtgcgtat caagccattg cccaagacga tccagcgttg ctgcaagctc cttcgccgag
    39181 aggttccgga tacggtcggg tcgataggtc tgcgccatgt ctttcatcgc gacaaagttt
    39241 gccgggccct ggcgcacaac cctccgccat tcccgcatca tcagcacacc ggcgcgtacc
    39301 agcgcgagcc cgctcaaagg acgcaccgat ggcgtaaagc ccaacggttc cccacccatc
    39361 tgctcggtta agaacgacgg gttcccatgc aattgtgtga cgagggtgta aaacaaggtg
    39421 acattgagat agggccgtcc atgcatcacg cgaacggatg agaggccctc aggaaccgtg
    39481 cacccgaggc gccgataggg cccaacgata taggcatcca tgaaccgttc aaggaacgag
    39541 aggcccagcg ggctcggcaa ttccggcatg gtttctttca aattcgcccg tgtccactcg
    39601 cattcatcat tcgtcagtgc cggggagggt tgcaccgcgg taatcggtcg cgcctgtacc
    39661 acccacacct tatcagcgtc ccacacccat tcgaggtcta tcgggtgtcc aaacgcggct
    39721 tcgatgtgct tcgacaatgt ggccaactcg aaaagctgcg catcagacaa tgaggactgc
    39781 ttctgcccgg cttcagaaat cgactcggtg tgcagtccag caggggtgga cagcaatttc
    39841 tgcactttcc tggccagcag acgccgccga atccgaattg gccgcccctc gtcttccgcc
    39901 acttcaacga catactggtc cggagtcacc tcgccgctga ccagagatgc ggcaagaccg
    39961 ggcacggcgt tgacgacgac ctgagaagtg cgccccgtga ccgggtggat cgagtaggcc
    40021 acacctgcgg tagtggcttg cagcagcggt tggattacca ctgccatgga tgggaaagat
    40081 tttgaactgc tcgtcctgag ggcatattgc accgcggcgt cgctccacaa ggactgccaa
    40141 caatcgcaga tccctcgcaa aatctcggaa gacgacaggc cgagggacgt gctatagagg
    40201 ccggccgcgc tggcttgctc gacatcttcg ttcgttgctg aggatcgcac agcccatcgt
    40261 gttgtcggat cgtgcgtgag ctcgacgagc cgtcgttcca atgcgatctg aaatcctgtg
    40321 ggccacgctt gtttcagcag gcaggcgtgc actcgtgcca gctcctggct ccgttcttcc
    40381 tgtgacccct gtagaactct tcgccagact tcttcggcat taacgccgcc tgcggccagg
    40441 acatgacggt agaccgttgt cgggacacac aatcctcgcg gtacggcgaa accagcggcg
    40501 agcaaacggt tcaagccggc agccttgccc ccgaccagac tcggatcgcg gctctcttcc
    40561 agagagagaa tcagtgatgg aatcatggag agggatagta actagagtgc gaggaggagg
    40621 taaagatcgt tgacgttggt cccggtggga ccggtgacga tatgaccgtg cagtttctcg
    40681 aagaatgggt aggcatcgtt atcttgcaac gcctgagaca ggtccagccc ctttcggcgg
    40741 gcccgcacga ccgtctggcc atccaccaca gcaccagcca cagacgtcgg cccatcagtc
    40801 ccgtcagtcg caaaacccgc cacccacacg ttgggcaatc ccgcaatctc aggtgcggct
    40861 gccaacgcaa actcttgggc tcgtccgccc gttccctccc ctcgaatggt gacggtcggt
    40921 tctcctcctg caatgatgca gcaggggcgc gggatgggac gtccatgccg cgccatctca
    40981 cgagccatgg cgccgaacat tttggcgagt tccctcgctt caccggtaag tgtcgtggac
    41041 aggaccaacg tgcgtagccc ttgttgctgt gcagccgtgg cgacggcatc gactgcggct
    41101 tgattgtctc cgatgagtat gttgtcgacg cggcgaaata ccgaggcgcg gggtttaggt
    41161 gtctcgggtg cacggtgtct catccccatg ctgagccgat tccgaacaga tgccggaata
    41221 gttttgacga ccccataccg ctcaacaatc tcccaggcct cccgaaaggt cgttggatca
    41281 ggcgcggtgg gccctgagcc aatcgtaccg aggtcgttcc cgatgacgtc ggagagaatc
    41341 acgctggcca cgcgcgccgc agtcgccgca gcgagctgcc cgcctttgac gctggacagg
    41401 tgcttgcgta cggcgttgat ctcatgaatg gtcgccccgg accgcagcaa taatttggtc
    41461 gtctgctgtt tatcttggag cgtaattcca gacacggggg cagggagaag gctcgacgca
    41521 ccaccggaca gaagcacgat gagcagatca tccctcgtga gcgtctttac caccgccatg
    41581 acctgacggc cggcctcaag accggcgaca tcgggaatgg gatgcccggc ctccacgata
    41641 cgaatggtgt tggtcggcgc cccatgacca tacttcacca caaccaaacc ggcgtcgatc
    41701 cgcggcccca gctggtgctc gagcgcctgc gccatgcggg ccgacgcctt cccggcgccg
    41761 accacgacga tacgtttcac ttgattgaga gaatactgct gcgagccgat ggtgagatgc
    41821 ctgcgtccga tgctgacctg acgccgcacc gcctcatagg gatcaaccgc gtgcaatccc
    41881 gcctgcaaca gcctggcgag aagggctctg gtgcgaggct gcgggacgcc gatttcgatc
    41941 atgcgaacta tggaagttta tcttgcttga aacagcggtt cggacaggtt tgtcggggca
    42001 ccttaacctg ataactcccc tttggcaagt agtccttcgt gaactccgga ttgagcatct
    42061 tcagttcaag gaaataggtg ccatactctt cggcgataga ggtgagccgg cgctgcgcct
    42121 ccttaatggt cacggtcacc gtctctgttt caagcggcgg atagagatcc ttcttggtca
    42181 agccaagata cttctccggc tgggaataga tttccttcgc cgcaataatg cgcgcgacat
    42241 accgcatggt ttcccgcggg ccatgcatgc gccagaaatc cgtcacattc tgttccttga
    42301 gcagttttcg cacccggtct tcgcccgcgt tataggacgc catggctagg aaccagtcgc
    42361 cctgcagttc ccgaagatag cggagatact tgatggccgc ttcagtggac atctcgagat
    42421 tgcgacgttc gtccagccag cgttcattgt gcagcttgta gcgcttaccc gtggagttaa
    42481 tgaattgcca tggacccgac gctttggctc gagaataggc cgcggcaatg cacttactct
    42541 cgaccaacaa catgtatttc aggtcatccg gcaagccggc atcagcgagt tgcttttcag
    42601 cgggggcaaa gcaccggccc gttcgcttcg ccaggataat gctttcgcct tgatcctcga
    42661 gaaactgata gaactcgtat tcgatgcgct cacgcacctg ccaattatcc agtggaatcg
    42721 actgcccggc gaaggtcaac ttgtcgggca gcttgaatga acttaagaaa aagcgctcgc
    42781 cctcgcgttt gatttcagga aggatgacga gccggtcttc gagctcaggg gcaccctctg
    42841 gttcggctga cagggggcct gttccgcgtt cgggtatggg aacgcgggag tcatcgctcg
    42901 cgctcggcga agaaggcgct ggggctttcg aatcgagaga gacttctgca aacaacggtg
    42961 ccaagccgag caccatcgtg acaccaacta cgaaggcccg aaggccccaa tcattgttcc
    43021 gcatgcccga cgtatccttt catgacactg aaagccccat gctatcaaca agcccctgac
    43081 gggacaagaa actcgtcggt ttgatacact tcaccatcat gtcgacactc tcgatgctca
    43141 atgctgacat cattgcctgc actcaatgcc ctcgactggt cagttaccgc gaatccgttg
    43201 cggcgacgaa gcgtcggcaa tatcgggagt ggctgtattg gggaaagccg attccgggat
    43261 ttggggatcc acgtgcccga ctgtacgttt tggggttggc accggcagca catggaggaa
    43321 atcgaacggg acgggtattc acaggcgatc gcagcggaga ttggctgtat gaggcccttc
    43381 atcgattcgg ctttgccaac caggccacgt ccaccgatgt cgatgacggc ctcgctctta
    43441 cggactgcta tatcggcgcg actgtgcgat gcgcgccgcc tgcgaacaag cccacgccgg
    43501 atgagttcac agcttgtcgc ccgtttgtgt taggtgaact gcgcctgctg gagcacgtgc
    43561 aggtggttgt cgtgttgggc aagattgcgt ttgatcacta ttttaaagcg tgtcgcgaga
    43621 tggggcgaga actgccctca cccctgccta aattcggtca tggcgttgca tatgagttag
    43681 cttggggggt atccctgatc ggctcgtatc atccgagcca gcaaaataca tttacgggca
    43741 aattgaccag gcccatgttt caccgcattt tttctgatgc ggcgagccgc cttgagggga
    43801 accggcgtgg gtccccctcg ggttagttgc aactacttct tccccatcgc gtcccagtcc
    43861 gccaggaact tctttagacc gatatcggtc agcgggtgtt tgaccaactg ctgaaacacc
    43921 gaatagggca tcgtgcaaat atgcccaccc gccaacgccg cttcgacgac atgctgcgga
    43981 tgcctgacgc tcgcgaccag gacctgggtc ttgaaatcat aattcttgta aatcgtgaca
    44041 atctgcctga tcagcgccat tccatctgag ctgacatcat caagccggcc gataaagggc
    44101 gatacgcacc aggcgccggc cttcgctgcc agcagcgcct gcgtgggaga aaagcacagg
    44161 gtcacattga cacgaatccc ttcggcggaa agcttcttgg tagctcgaag gccttcagga
    44221 ataagcggaa cttttacgac gatattcttg tgaaccttgg ctaaatcccg accttcttta
    44281 atcatggcct cggattcgac accgacgacc tcggcactaa tcggaccatc gaccatattg
    44341 cagatttcca gcaacatttc cttgaaacta cgcccttcct tggctaccaa agacggattc
    44401 gtggtcacgc catcaatcag ccccaggttg gcgccttctt gaatttcttt gacgttcgcc
    44461 gtatcgagat aaagtttcat gaaagagtcc tttcttttcg cttcaaattt tcacgttctt
    44521 gtgtcacgtt ttattctaga agcaagtcaa aggcaaggca acgggtcaac tttcctatga
    44581 tagactcgtt tgacacatca acatctcgca gaatgtccgc ttcagcgcgg acctaatcag
    44641 gagggctagc cgtatgcgcg catccgtttg gcttctagcc gtcggccttc tcgtgggtgc
    44701 ttgcagccat aatccttact tggacaattc attgacccgt tatcaaggga aagaccgcgc
    44761 gtggatcgaa aaggaattgg ggcctcccga cgcaaaaact actcgcttct tcggaggaga
    44821 gaagtggacc tacaatcgaa ttgccggggg aaaggcagga ccgccgctgt ttaatttcaa
    44881 ggcgaacgaa tgccagatga tcttgttctt tgataaagat gataccgtct cggactcgac
    44941 ctattcaggc tgctagccag aaagctgctt ctcaaatgcg gccgcacatc cgcggctgca
    45001 aaaaaaatag gtttgcccac caatgttttc tttcacggca ttctcgcgag cgacaaagac
    45061 gcgacaaacg ggatcttgta ccatttggtt gccggagagt ctgccatctt ttggtttttc
    45121 cagtgcacca tcctgcccaa tctttctgat cgccctccgg atcaggtagt aaaggacgat
    45181 cagcagtacg gcaatcagaa agaatctata cattgtggta cttccctaac ctacccacga
    45241 aatgtccagg ggtgatccct tcattcattc ccgccacctg gacatattac gagcatggaa
    45301 caatccctta agggcattac taggaccgaa tggcccagat acttccatgg ctatgagggc
    45361 cttttcccct cgagcagaca gtacttcaga tggatggttt atcctacagc gtaatgttat
    45421 aagccatcca tgcataaatg tgacaaatgc cgcggaacaa tgttgccgga acgggcggtt
    45481 gatctgaatg ctggtttagc gatcacggtg ctggcttgcc tgaactgtgg acgccgaaaa
    45541 gcagccgatg cggaaccgag accgatcacg tcgcgacatt gagccgatgc agtaacattc
    45601 gatcttagag tgctcacttg cgatgagtag ggggtagcga cgtaccctct acagagtacc
    45661 gtcctcctct ggacttccgt accaccccat tgcacgataa gcctcctccg tttcatacgg
    45721 ccaacataca gagccaactg ctagttgcgc cgtgtcttga acgaacagca ctcctccctt
    45781 attcaattca gattggttca aatataggcg caaagggacg ggatccgccg gcgcttcctc
    45841 atgcggaaat agccgtacac cttgcctgag atcgaattcg ttggaaacaa cttacttgag
    45901 actcaacaca tccagcatgt cataaagacc gggaggttgc ttcaccagcc atctcgcggc
    45961 tcgtaatgca ccccgtgcga acgtatctcg actgctggcg cgatgcgtaa gctcgatacg
    46021 ttcgcccatg gttccaaaca tcaccgtgtg gtctccgacg atgtctccgg cacgaatggt
    46081 ctggatgcca atctctccct ttttacgctc accgatcaat cccttgcggg catacacccc
    46141 aacctgtccc aggtccctat tgaccgctcg tgccaggact tccgccatct tgagagccgt
    46201 tccgctgggg gcgtcctttt tgagccggtg gtgcgcttca atgacttcga tgtcatagtc
    46261 ctcgccaagt gtcttcgcca tctccgcaat cactttgtag atgacgttca cgcccacgct
    46321 catattaggc gagaacacac atggaatttg tttactcaca cttcgaagtt cgtctaattc
    46381 agatggggaa aatcccgttg tcccaaccac aattccacgg cgatgctgag ccacgatcct
    46441 taaatgcccg agcgtcgccg caggggtcgt aaagtcaacg acgacttccc ctcgttccat
    46501 cagacttgaa agatcctcga cgatcggcac gcctgtccgc ccgcaaccgg ccacttcccc
    46561 ggaatcctca cccagagcat gatggccctt gctctcgaca gcccccgcca atgtcagaaa
    46621 tgccgaatct ttgaccaggg acacgagacg agaacccatc cgtccggcag ccccagtgat
    46681 aacaaccttg atcatggcac catccttcag aacgaacggg ctaactacac caggccagcg
    46741 gccttcagta cagtcagcag tttcgcctta ttgtcatttc ccatggggca gagcggcaac
    46801 cgtatttcag gcgcaatctt gcccatcaga tgtaatgctt ctttgacggg aatggggttg
    46861 gtctcataaa acaaggccgt aaagagagga tacagctcgt aatgcatcgc gcgcgcggcc
    46921 tcgagtcgcc cgtcacgaaa tgcattgacc aattcagcca tcttcgtcgg aacgagattg
    46981 gccgtgaccg taatgacacc cttcccgcca actgccatca tcggcagggt caacgcatcg
    47041 tcccccgcca agacggtcaa ccgctccccg cagagctgaa tgatttctga tgcctgctga
    47101 accgccccgc ttccttcctt aatcgcaacc accgtcggac agacggtcag tcgcgcgatg
    47161 gtggaaggca tcatgttcac accggtccgg ccagggatat tgtagacaac gagcgggagc
    47221 tcgaccgctt cagccaccgc tttgtaatgc agaaacaggc cttcttgtgt cggcttattg
    47281 taatacggcg taatcagcag ggcgccatcc acgccggccg ctttggcatg tttcgtcaat
    47341 gcaatcgctt cttcggtact attggatcct gttcccgcca ccaccggaac acgacgtctc
    47401 acgacttcca cggtgaaggc caccacacga tcgtgctcag catgcgttag tgtggccgat
    47461 tcgccggtcg tcccgcaggg aacaatcccg tgggtgccgc tgttgatttg ccattcgatg
    47521 aggtcaccga gcgcacgctc atcgagcttg ccgtttttga acggcgtcac aatggccact
    47581 aaagaccctg taaacatggg acgctccatc aaggaagaga ataaaagccc gacttacgaa
    47641 aggaactccg gcacgtgctc gcctcggacc aggtcttcgt aagtctcgcg cgaccgaatg
    47701 acgtgatact gcccttcgtg caccaaaacc tccgggacgc gagggcgcga attataattc
    47761 gaggacatga caaatccgta ggcaccggcg ctcatcacgg ccatcaactc accagcgccc
    47821 attgccggca tgaccctatc ctttgccaaa aagtctccgg attcgcatac ggggcccacg
    47881 acatcaacgg ttttcttctc cccttgcgta accttctcgt acacgggacg aatatcgtga
    47941 taggcatcat agagactggg acgaatcaaa tcgttcatcg ctgcatccac gatcaggaag
    48001 cgtttggctt cgccgtcctt ggcatacaac actttagtaa gcatcactcc ggcattcccg
    48061 acgatcacgc ggccgggttc catgatcagc acacacttca aatcgcgcac gagtggagaa
    48121 atggcctcgg ccaggtcttt cggttccggc ggcgtctcat cggaataggt aatgcccaag
    48181 ccaccaccga tgttcacata ccgaagggga atcccttgct ctgccaacgt ctgcaccatc
    48241 gttaacactt tcttcaacga ctccacaaac ggggtcactt gcgtcaattg cgatccgata
    48301 tgcgcgtgca gccctaccac tttgatatgg ctgaatgccg ccgcattctt gaattcttca
    48361 atcgcccgat cagccgcaat accgaatttg ctttttttca agccagtgga aatgtacgga
    48421 tgcgtcttcg ggtcgatatc gggattgatc cgcaacgcca cccgggcctt cacgtccatg
    48481 gaagccgcca cctcgttaat ggcctgcaac tccgcagacg attcgacatt aaacatgagg
    48541 atgtcggcct tcaacgcgtc acgaatttct tcctgctttt tccccacccc ggcaaagacg
    48601 atctttgatg ccggcacgcc cgccttcagg gcacggaaga gttccccgcc tgaaacgata
    48661 tccactccgc tcccctcctt cgccatgagc cgaaggaccg cgaggttcga attcgccttc
    48721 atcgcaaagg caacaatgtg cggaatgttt ttaaaggcgc catcgtacac gcgaaaatgg
    48781 cggactaacg tgtggtgact atacacataa caaggcgtgc ccacttcttt cgcaatacgc
    48841 gaaagcggca cctcttcaca gtacaattct ccattccgat attgaaagtc atgcatggta
    48901 ataatcctcg atcaacaatt ctaaactggc ccgtaacaag tgtcggaccg ccgcggcgaa
    48961 ggggattcgc agaaaaatcc gtgactctcc tgcgcttatc tcgtccccat cgtcctccat
    49021 ggcgaaaccg gcagtggatc aggatccggt ggaatcgggg tctcttcaat cgtcagcccg
    49081 ctcggagcgc tcggccgcga ggctgatccc cgaacattcg cgcctgacgc taacaaccct
    49141 tcccgcctca actgccgttc gaccacgggt gccaccccga catcctccgg cggaatcggc
    49201 gcgcccaaga ccccgcaccc cgccagcaag atgaacgata cggtcgccaa taaggcgtgt
    49261 ggctgaatgg ccgcgcgcct tgcaccggcg gacatcatcc cttcagcgcc ttttcgagtt
    49321 ccttgattct cgcttcaaca cggcgccgcg ccgtcccgcc gatctgagct tttcggtcga
    49381 tcgcgcctcg cactgtcaga caattcaata catcggcctt aaagtgcgac gaaaatccct
    49441 gcaactcttt aagcgtcaac cgttgaagcg gccgccgctt ctccagtgag aatcgcacaa
    49501 tctggccggt aatggaatgg gcctcccgga acggaacccc cgtcgtcacg agataatccg
    49561 ccagttccgt ggccaacatg ccaccacctt cggccgcctc agccatgacg tcccgattga
    49621 cggccagccg ccgcaccaac tcggtgcaca gcaccagcga ttgtccggtc gtatccacgg
    49681 catcgaacaa ggcttcctta tcttcctgca aatccctgtt gtaactcagc ggcaggccct
    49741 tcagcagcgt cagcgtcccc atcaaatggc catacacccg cccggttttt ccccgaacca
    49801 actccggcac atcaggattc tttttttgcg gcatcatgct gctgccggta caaaaggtat
    49861 cgggcaggtc cacgtagcga aactcctgtg aggcccagag aatcaattcc tcactcaacc
    49921 gcgagagatg catcatgatc agggacaatg tact
//
LOCUS       DAOQDS010000030        47973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_237_length_47973_cov_20.147711, whole genome shotgun sequence.
ACCESSION   DAOQDS010000030 DAOQDS010000000
VERSION     DAOQDS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 47973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47973
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_237_length_47973_cov_20.147711"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2952
                     /locus_tag="PK589_09210"
     CDS             <1..2952
                     /locus_tag="PK589_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19820.1"
                     /translation="WQGTRAVTEPENTVPNPRYQSAHPGERLPIVFEPLTGKVSWPHL
                     KPHFGRRVPFSKSHNPSPWLEMIHLDEDGQPSSYPAKPGENGRWSLCPENAGSKKYNV
                     HFIHTPMMLADKQGDTAAIMDEGGLIYVLHEEEAAIRKNNDLKFPLVVRSNIYDCVDW
                     MLTSEWDDDDFGNFHASKVNTHWHFLQFDNQSSDGVITGFSNEQSVRPFTMLEKKTDK
                     GLPLPLNTTFTKAGKKGDRVITLKNAAQYHPGIEILIGADNVGGNEVGRIQSIKGNEI
                     TLVRPLKNDHPVKDIVTVEFVRQRFWVDSDVGTVFWHDHALGRITWGHGGFGTMLVEP
                     VGSTYHNPKTGAPIRSGPLADIRTAEPVGYGVNGSFRELLVQLNDSVPHTINIVTAGN
                     PPGQPVEVALEAGKTISFPIPDHIKMTPMPFLNGGTHTTGGGLNFRAEPISGRLKANP
                     DTSKLFSSAVHGDPYTPMVRAYLGDTVVFRLLQTMTNESMVWTLSGHTYLTERYAGDA
                     NRKNSIHVGIAERYDLVVPQAGGPRLQAGDYIHFNGRSSKFSEGAWGILRVLDKDVTD
                     LQKLPAGYSGRNEIPKAPAVCPADAPVKSFNVSAIDFPSMKLNPKAPDAIEVDFERTI
                     QMVNPEAKIYVLDEDVATVASGLQPMPLTLRANVGDCLKVKLSNKMKQGRASFSALGL
                     AFDPKDSLGANVGNNPGEQTVAPGESRVYTFYADPFIGETASLVWDWGNVMTNPRNGL
                     YGGIVIGPKGATYRDPKTGADISTKNSWIADVIVDRTIQGYETRQNYRDVALFFQDED
                     NIIGTSFMPYVQNTAGLTAVNYRAEPYKFREASGCSLGKVFQPCSVDKPESIATPIIE
                     AHAGDPVRIHVFGASNEQNGMFSVEKHEWPIEPFMRGADQISVVEFSASESLDAFIQA
                     AGGSFRQPGDYVWSNQRLPYAQSGQWGLLKVLPTDDQRILPLSQQAPSVKRAGSESGE
                     AHASKTSHLIQ"
     gene            3222..4202
                     /locus_tag="PK589_09215"
     CDS             3222..4202
                     /locus_tag="PK589_09215"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HNJ19821.1"
                     /translation="MSALLILLLTIAGWSFTAQAWAFGDLGHRTVGAIADAQLTPETR
                     AAVWTLLRSGETLATVATWPDEVKRAKKNLGPLAHDAEAQRFNEDFPDNREWHYVDLP
                     LDTAVYTEGAVGSRLNDIVQTIQRCIRVIESQTVSDHEMTRAQALRWLVHLLGDLHQP
                     LHLGSGYYQFDAQRQAHLITDPHEAAGKDEDRGGTLLLTAAEEQLHAFWDDRLVNEVA
                     KGRSYLELAQDLLAQGHQGAWDGDGSYHHWVDQWAVEGIRLGKSAYERLVFGTAQFDG
                     DHTGLRKITITLPRGYEQDEAKIVEQQLAKAGYRLAAILNALRFPAAASH"
     gene            complement(4365..6596)
                     /locus_tag="PK589_09220"
     CDS             complement(4365..6596)
                     /locus_tag="PK589_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNJ19822.1"
                     /translation="MTHRFSTRTILSLAVITLLVVQPVPSFTTEPPSSGTKRALLIGI
                     NRYQVLPTLNGSLNDIDTMRQVLTTRWGFQSQHITMLTDQAATRSGILAALERLVQDT
                     GPSDVVYVHYSGHGSQVEDLNGDETEDHLDETLVPHDGRTPSVPDITDDELEAIVSKL
                     KARSILIVLDSCHSGTATRSLDIRTRSVPQDTRIELYRPSGVHTRQVVPVTAARYVLM
                     TGAAANQEALDGPVGGRYHGFFSYSLSRSLGSAGLSASPREIFFGVEREMKRIQTHFG
                     RISMPEPQLEAPPALMDAPLLPMPSETGNETGTNGTARLPWVEVKAHKGGETLLLNGL
                     QLGAAPGSTWAVYPPNETNFAPGKSLGLLTVTQSRGKDAVTAPHATAFTIPTGSRAIE
                     FLPPASVEGVPVRLLEIPNDRREKIEQVLREQGREVQIVGPGQPAQFLVEVKGNRLQL
                     YSADGLQLVSSFSLDNAQWGAGLARVLSRSANASELLTLDNPSAQIKLDVRIAGAARR
                     QARGIAVVADTQPAQYRIRHEGEPRTPGNSLQLEIQATADSYLTIVDVDSEGHLNLLF
                     PTVHQQPSFYPEGRVRAGDVVTIPDSLQSGNHAGFHWDYAPPAGTDTVRVFASTDLET
                     AQAIRQRVIATTPPGPLATTMVTTRSILSSSMTHLRQDLSRIATRGLSSVYDPTPAAL
                     TNQPSTPPTQTPTAPTAGPSSPQPPVSQNLSPPIAPSTAQTSALTPAADWAATSLTIE
                     VAP"
     gene            complement(6646..7323)
                     /locus_tag="PK589_09225"
     CDS             complement(6646..7323)
                     /locus_tag="PK589_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNJ19823.1"
                     /translation="MTTPHNLAPPHPTATLLPWYLTGTLKESERQAVDEHLASCADCR
                     AELAHLTHLRTPVKTAFAEETTPELRVQQQVMIRIQAETLPAHSAGPTTSKEGAAESV
                     EQWFRRLFAPRWIPALAAILLIGQLTLLLWTAGEQTALPPSHIASRGIQPVSSKIVLV
                     FQDSIPEARIRATILALKGRLVDGPTVDGRYTIEIPSTETAALDQQLHTLQQQSAVIR
                     RAERLAP"
     gene            complement(7320..7904)
                     /locus_tag="PK589_09230"
     CDS             complement(7320..7904)
                     /locus_tag="PK589_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ19824.1"
                     /translation="MDATHGHNALERTLVARVATGDTQAFEALYRLYDKRVYHYIQTF
                     IRDAALAEEIVGEVMFALWRGACTFTHQSRVSTWIFGIARHKALDAVRRHGRIDHSTA
                     PLDDVPDLPSQQETPIDGIQREQLGIVTRQALSKLSAEHQEILRLVFYEELPYEEIAL
                     LLSIPENTVKTRVFYAKQQLKRHMDRMLSRESVV"
     gene            8074..9675
                     /locus_tag="PK589_09235"
     CDS             8074..9675
                     /locus_tag="PK589_09235"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNJ19825.1"
                     /translation="MHTRLQPLRTASGTRTSLLIFLPFLLLASCAAAPFLIPAGIEFA
                     RNLLVTSNKNYGGKYSEDMNRLMVRLSTPYVAMGLPMGTSPAALVPPTMLMQQQQAMM
                     NAQASGQAGMGMGMGYPDPYNPAGAMMGGYGAQGYGGMGGYGGMSGYGGMGGYGTSQY
                     GMSQYGYQGQAGGYYDPNNPYASVPGMNVQAPAYGMPQPYGGQYGAQSYGGQMGGYPP
                     QPGYPAQQGLPPQPGYPSAAPGMAAQAPAPGYPPQGMQGYGQQPMQPGMYQQPMDLYQ
                     QQAMQGYQQQPMQQAAMGGYQQPAVQGYQQPVQGYAQPGMAQPYGQPPNVGVSYPAGV
                     YPRGIPGPAEPVALDVALIRQVNGSNGKQVALMQDGETLKGGQDGDRFKLVVRTNCEC
                     FVYVISIDGSGWAQSVFPVANGPVNNPLKPEVEQAFPDGPYWFTLDQFKGVETFFLVA
                     SPARRTDLEESLGQLAGQQRPAGQVAAQVEEPAVIPNGFGKTQSGQAAQVLDDGQQAM
                     QVTPLSYVAAKVGDDVRVTRWFRHQ"
     assembly_gap    10070..10169
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10170..11011
                     /locus_tag="PK589_09240"
     CDS             <10170..11011
                     /locus_tag="PK589_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19826.1"
                     /translation="QSVPAQPAPPAMGQPMPGPRQQQPVMPGPGLQPMMQPPQVTSPL
                     VKTIPLALLLGGDLSKSFVQLTNLRGQVDSNFTIGRSLAGRFTVPPYVHEASNGSRVF
                     VHANGLSNPPVVKSEGNELHLEFHLPALQFKTYYRDYSPEGDQAVGDTVGENAIIDIF
                     ILPTTNQQGFPTYQAVRAGFRGTLKPQDKCTYWFDVIFPINICEVSKKYLDTLSNAIE
                     TGVREALSHPQTRTQFDQIVWQSLRSDLLIQAGINPMSPAQIRVIEASFHGTDYVVKY
                     LPR"
     gene            complement(11145..11600)
                     /locus_tag="PK589_09245"
     CDS             complement(11145..11600)
                     /locus_tag="PK589_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19827.1"
                     /translation="MRSDRVVFIGAISLLTFAVILPAPASARPKGTWQSCNTKDLNTN
                     FGASCNDQMQQDIMGNKPYTHVLFCGGETMLCCTVDNNTNQVINCRKPAGSRVMPGLQ
                     GNTLGASGMAGVQRRGVESKAQDSDEDAPVPSTLTSDVVKELRPKAAAK"
     gene            complement(11686..12918)
                     /locus_tag="PK589_09250"
     CDS             complement(11686..12918)
                     /locus_tag="PK589_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19828.1"
                     /translation="MRHTAVVTFVGTAALCLGLAGCAGTDHSKDQAARDVPAREVPVI
                     PEDAPATQDAIQSAESAEVQERGVSRVLPGGTAGAAVLGTLSTPFTSAYPGEFAFRTQ
                     KGYYLTAVNGGGRTGDPTIITGATSAGAWEKFRIGIATPPSPYDRLLQTATGNYLTAV
                     NGGGMTANVLHTDATQPKEWERFRLVYMGGYPPPYYGIGTSKGNFLTAVGAGGRYQDA
                     IHSDARQVQAWEQFRIVKCGDPGSGYEYGIMAADSELFAASTYQGKTWFGLNGHPSEA
                     EMKLKVIRQGDGTYALQTTSGRYVTALGGGGQVEKYDRKYWTPDCGGLFGGPCVGGAS
                     PIFHIDATQVQAWEKFRLVDQGNCTYTIQTTSGFFAGIFKDGSGVTLLTTRRDGAPTA
                     NEKFQLVVFGLASPPIIQ"
     gene            complement(12998..14065)
                     /locus_tag="PK589_09255"
     CDS             complement(12998..14065)
                     /locus_tag="PK589_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19829.1"
                     /translation="MTIVRYPCTACLVFVMAWGLSMACQAQDNQDSLPSSAPAQEARP
                     NSTEGTTPDEGIKIQEPDLITDDHHPVTGEIQERGIVDKFQATPGKTFMQAQYQAPTA
                     NLTLVANALRLAYKSVSTLVTIAPNLPVTQPVEISIGYYSAAGNDRITQSYVRASGNR
                     FLYNDKEGDGKPRTMTISISLREPKAGGGFETFAVNWQAPLDPLYDVSIGPFAFDLIS
                     KCDAVGKSEILFTWYAPDQQYHKFRFSTRAGSRTTISPFAWSRQEVSWSHKVSRPTET
                     FYDEDNAATEMLWNCLPAGCGFTVAGGPSAQHLLTGASAMVKGNLKAMNDNCQAYFEY
                     PMTKTLRWYPYLDSPTVRDHR"
     gene            complement(14152..14613)
                     /locus_tag="PK589_09260"
     CDS             complement(14152..14613)
                     /locus_tag="PK589_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19830.1"
                     /translation="MTKPYAITTVSLMILTGLLALPAASDAKPRKPLVNGCTVDQIQS
                     NFGNSCVDQMQQDIMGNKPYTHALLCSGGQMLCCTYDNTTNQIQTCRKPAGSAFMPGV
                     QGSVGVLGSAGIQRRGVDANTAAEAEEAPAPEWMTPERMQQLQQDFKTNGQ"
     gene            complement(14669..15103)
                     /locus_tag="PK589_09265"
     CDS             complement(14669..15103)
                     /locus_tag="PK589_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19831.1"
                     /translation="MHRLCSHPSLMAGLVCGVIVAAAPALAVPIKNPDNCKGPVTIGV
                     ATCPDKPGHWVECIPSGDYMCCVQNAQGGKDCEQIEALKGPRGGMTRVPGGMLQNAPV
                     VSPAPINPRVPRTGMNAPIMRRGIEDKPSGDGRPGTDDNENK"
     gene            complement(16388..17554)
                     /locus_tag="PK589_09270"
     CDS             complement(16388..17554)
                     /locus_tag="PK589_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19832.1"
                     /translation="MNRYLAIVVTGWLLAGWAGCAADSPSSNSNATTIAHTEEATGQP
                     QADEVQERGVTPKPLRPGLTSPQVFTPIPSAPNTASNPPAFGLLPGDFAIRSYLKNTP
                     LTARDGGHHSIDAVITLDSVIGPNQRFRLATVQPDYTTIQTNGGYFVSAVGGLGGLPN
                     TTQGVQTELQTPQRDIALFRIDGPSPAGTFTIKTYDGHFLTALGGGGKSTDAFHTDAV
                     QANSWEYFYFLKCGDVGSGYQYAIRPTGTGNIPGKGDFVSYLTALNGGGMASQAVTAF
                     SGLQVGSRFRLLRQSDGSYALQTSNGVNYLTAVGGGGIASGDNLHTDARQIQAWEKFK
                     IVEQPNCSYTIQTVSGFYLAVGPGSSSISTRISDPNAAPSIGYNAKFELVMVGL"
     gene            complement(17742..18497)
                     /locus_tag="PK589_09275"
     CDS             complement(17742..18497)
                     /locus_tag="PK589_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNJ19833.1"
                     /translation="MNRCHSLIATTALILLFTHATLAGSATSSELDPAARQSMEQVIE
                     EYIRAHPEVIEQSLQALEAKREAEEKAKQKVALAAHQAELLNDPTSPVSGNLSGDITL
                     VEFFDYRCGYCKRAAGAVTQLQQENPRVRVIYKDFPILGESSELAAKAALASKAQGKH
                     QVFHEAMLAFKGDITKESLLSLAGEVGLDTIKLEADMRNPEWQKAIDTNRALARTLGI
                     SGTPGFIVGTELVPGALDLNGLKELIARANHRP"
     gene            complement(18813..19280)
                     /locus_tag="PK589_09280"
     CDS             complement(18813..19280)
                     /locus_tag="PK589_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19834.1"
                     /translation="MTRISRRVLFIYLSLAVLVSATPTSLSAAPPNVDPCTLLTIAEV
                     EQTVGALKGKPTGDKEGDAAWCNYEFANGKDALEVWVFPSGAIGRSRAISKNSVTVKG
                     LGDEAFMDRGMHGLNYVNLYVKKGSTTINLSIQETTGDEEKVKALAQKALRRF"
     gene            complement(19324..19782)
                     /locus_tag="PK589_09285"
     CDS             complement(19324..19782)
                     /locus_tag="PK589_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19835.1"
                     /translation="MRRSRPLLLLYALLCLGSALISTPSLAGPPDPCSLLTPAEVEQV
                     VGKLNGAPTSETLGHGVRCGYNFADEKNEFEIWTSEASWSKALRKDAKQPVMVSGLGD
                     EAFMDRGKVDPEAVDLYIRKGATLVLLMTRNGPRVEEQLKTLGRKAIGRL"
     gene            complement(19842..20825)
                     /locus_tag="PK589_09290"
     CDS             complement(19842..20825)
                     /locus_tag="PK589_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19836.1"
                     /translation="MRPHWRLGIWLITAPLTVAGVGVLHAEQPAIQCGPPRIEASFTA
                     ALSSYHVTTSCSAVPAGFVNWDSRGSYDSHTGLAREEVHVYLPQGFSATATTTVTCPS
                     DPWLGPAVGAGKVACANGSFNTSGQAQDARARFWLDWLRDGFSGSALPNSTGFSYNRP
                     PLIAQRDAALKTETDAAAALQKHNQQILGAARQGPAVVAPVIVLPAANASFLENTTIP
                     IKLLPPAGMQVTSFMVRLERRDSQGNWAVFNNLPVNLAEATSPTGYTGWGAPGTGRGP
                     AMIAGRGSYRVSAQVLSPKPTRWSVPVEFTVTALNKAIQPGPKAFSPGVVR"
     gene            complement(20885..22171)
                     /locus_tag="PK589_09295"
     CDS             complement(20885..22171)
                     /locus_tag="PK589_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19837.1"
                     /translation="MARCPARSAKRLRPDVNGLGLYVLSGALLVLSLGFFPSLSSATD
                     LELIGGPGGSLFRAECPQGSYLVGLAGRTGEWVDRIAPTCAPWLRGSQAFGLPTVGPS
                     FGASGGGQDRQRTCWGTGNKTLAIQSWYVETVRSPNPPVQYVEIHCASLGPAQSIGLF
                     DFGNPTAASEERVTKGPYPHGCPPGEIASGFRVRAGLFIDSLGLICAPIPARLGAPAG
                     KLATPLVQTPPPSVPFNPQAKKLQVPDDLFVITKPVPGERVTQGQLFIAAIPPAVGVT
                     NVAELELRYLDAPPGQKDSYPFVSIMAVNTTKLTQGYLVAPIVTDAYSGRWQVRARAA
                     MKTPPGPWSSPVPFQLTPGAPNKSLTPALQQTPLSGSSVMQAPPATAGGAASSTRPPT
                     TSSQGGASSLFVRPRGVEGTSKKEGNAPAPQSQREK"
     gene            complement(22183..22866)
                     /locus_tag="PK589_09300"
     CDS             complement(22183..22866)
                     /locus_tag="PK589_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19838.1"
                     /translation="MNWSLIAGIGFSLTVLSPLEAHAKGDLVAPNDPTCYGSEPKNAT
                     FVATRYTYIFSGSCNLAQTRLAIRVSVPWTGVGTFDPTTGQAAEDILVPAPKISEPSR
                     PYGRFKTTMHCSSDPWLNPTVQCDQIVPTVDAPLDRTAPNAQGYRPEPLAPNIVDIVR
                     ARRRPFTSFMNQDAVNSLNRRFGAYQAQNKTNQVIRQFKKVPGILGRGIEQGDQPSQE
                     TPGTGPSSK"
     gene            complement(22898..24076)
                     /locus_tag="PK589_09305"
     CDS             complement(22898..24076)
                     /locus_tag="PK589_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19839.1"
                     /translation="MTPAPARNMSLTWIVGVLGIVVLHCALAVSTEVAVADEPAVVGQ
                     LSMTCSVNTINGTHKPLSSDYAITGQCLLFETSSRGGTAQVNNVAVHWTVQASHQPNT
                     KATFETMNLALSEFGKTTATGTVSSTMQCGSDPWRIMPNGPCRLVTRKTSPPSFPGYV
                     SMAYQQLLQEAPNLPLSFKLNPAQRAAIDKQYHIAMAAQQTFDPSQTTLSKPQGPGVA
                     PFTPGGPNTSDAILSRQMPRILSPASGATVVVGQLLVKIQPPLIGKEPVAELEFAWLD
                     TPAGQPRFVNTIPAMDTAKLLQGQYIDPKVTRLNYGRWEMRVRMIGQGAVAGPWSAPA
                     SFTLVPNQQNQSIQTPSPASPLLKSPSERFGAGTSVIRPRGLNREKSEPSDSAATPAE
                     "
     gene            complement(24117..25220)
                     /locus_tag="PK589_09310"
     CDS             complement(24117..25220)
                     /locus_tag="PK589_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19840.1"
                     /translation="MNIWHRHAMTVVAGLVLIVALASMCFGFGFNGVGSAPCSSATQQ
                     MVGSFSSTKAQYVSTGTCTTPGSLGAPRTFPYTVKGAYANNIAEEVIEIAAAPISQPS
                     HPYGTWRTTYSCPSDPWLTMDGPPFGPESLRVKCQIVNRMDNSPSEAYPRKYQDGKPL
                     PTLSDLFNAWRAYKPMSAWVLMPAERTALAAKRDADLKAEAAALAKAQAEKRLRSATQ
                     TLTPYRMSLSPIITAPTAGQRFFNQSPISIKLAPPPPWADTEVRLDGTPVKTAKSVTG
                     YMVRLERKDPNGIWVPHATLPVGAAQAESSTGYTGFGAGAPPAFLSIPGAWRLSAQVT
                     TPQQSGWSDWVEFGVMAPPTNKILQPATKSFAK"
     gene            complement(25260..26285)
                     /locus_tag="PK589_09315"
     CDS             complement(25260..26285)
                     /locus_tag="PK589_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19841.1"
                     /translation="MQRHMWGVLLFVLSAIAGDASAAGVVVRIYPMTTVSCHLDSIQA
                     KFTAAKSTYRMTGRCDQQLKYEDHVEVIPIPWTSEGAYAPGQGKVAETIQLNGNPPLR
                     GKIETRLGCVEDPWLVMGKAACTQPVFSSTGDVQSLDYIVTYLRNGFEQTLKPNTTGF
                     SYDRAALVAQRPRDLQAEQQALAETARLAQEAARKEAQQLQKKVQPGSTLLENLAPFV
                     VSPTAGSHYLGMTTVPIKLAPPPGRVTVTLYTVKIERKNPGGTWALVTALPIGLAQAS
                     SPSGYLGWGAGGPDGRSLSLMASPGTYRITAQVSAPQQTGWSQPAEFVVMNTNKAIQR
                     APKTFGP"
     gene            complement(26338..27975)
                     /gene="lnt"
                     /locus_tag="PK589_09320"
     CDS             complement(26338..27975)
                     /gene="lnt"
                     /locus_tag="PK589_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNJ19842.1"
                     /translation="MTAHQRLRVTDGITPIALALLSAALLVVSLPSPDIGWLGWGALV
                     PILLAIQGLSPGRAAALGLLTGVLASFGMYGWLFEVPSFDLRHAVLLALYVGAYPAIW
                     ASATSWALRRDIPLLLCAPVLWLLIDYLRGHAGFLALPWGTLAQTQHRNLALLQTASM
                     IGEHGVTFLVALGNAALAALCLRRERTYATVALLILVTAHAWGATELFSPASGRSIRV
                     AALQPNIQVGDRTTEAGREANLARLERLTREAASARPDLIVWPESAIPGDVSDPALLH
                     RLQQLSSDTAVPLILGAAEVEKFATGDNLLTIGRRLFNTAHLFQPSGATSPPYRKRML
                     VPFAEYVPQADVIPWPEWLAPRVTELTAGDSGQLFTIAPQMSVAALICWENLFSHLAR
                     ESVNGGAQLLVQLTNDVWFGPTAAPRQHNLMSVMRAVENRVPVVIASNAGPSQLIDGY
                     GRVVAATSNVFQESAITGAVALGGGGTVYSKTGDWFVLFVLAGAFCTYVLLKRAHPGR
                     YANRPSFNWIRGVPGSLVVASMLGRGRRVLGRATPER"
     gene            complement(28103..28744)
                     /locus_tag="PK589_09325"
     CDS             complement(28103..28744)
                     /locus_tag="PK589_09325"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNJ19843.1"
                     /translation="MTSSLLRSTVIMLGLLAGGVAPAWAANAPVGWAVNAIGTLTIIR
                     ADGVEEQLQGRKTLPLYEGDVLKTDASSQAYIQLKQGVNVALNEETSFKILSRWQKDK
                     PTTWILRLSQGEVWAKTAGDGPKAFEVETPVATAAVKETEFNLKVQPDGQSILTVIEG
                     VVQFGTAFGTCPIRTNTISYGVRGKKCTKPAESDAKAAKAWTTTLIPPSAPVQ"
     gene            complement(28840..29835)
                     /locus_tag="PK589_09330"
     CDS             complement(28840..29835)
                     /locus_tag="PK589_09330"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNJ19844.1"
                     /translation="MSSSWARIGAATLGLIGLAGCAEVETVKAPIDIPSRIYTANESS
                     NDVSVIDATSFAPVGNIDSKNQSTHDIAVSRDGRRVYATNLASGRLSVMDAGTMETIA
                     SIYTGQRCHVVALTNDNKQAWVANIGDNNISIVDTETFRIVGTIPTGKGPTGLTFSHD
                     GKFAYVSTQGDKTVDIIDTATHQIVKSLQVGANPHFLVVGPKGYIWGTNTGGTDIYVI
                     DPTTQEISGTLEVGAKPQQIAFGFKGMQGPNVFVTVGGTNKVAMVPADPKNLKVLEEF
                     AVGEGPNGIWANPEGTRIFVGHDKGNEVRIIDTGTGQNLGTVPVGRKPIRVVVSR"
     gene            complement(29903..32998)
                     /locus_tag="PK589_09335"
     CDS             complement(29903..32998)
                     /locus_tag="PK589_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNJ19845.1"
                     /translation="MRVNLFVVTALLCLFTLLVPSTWASPSTTAPRLLSGHASEILTL
                     AFSPNGTVLATGGTDQTIRLWDPESGQDLKLLRGHMGAVHALAFSPNGKFLASGSADT
                     SVRMWDLASGRELHAVTSNFGAVRAVAFSADGKMLAAGGNDGSLRFLDSANGKELRSV
                     RGQFGIVFSIAFSPDGTMVATGSSDMQIHLWDLATSRQRTVLTGHTGAVHAVAFAPVS
                     HLLASASADGTIRLWDVDSGQERLTLNGHKGEVFGISFAADGRTLASVGADGSVRVWD
                     VVSGRERYALTSHKGPARTVALSPDGNVVASGGRDKTVRLQPSTPSTVTANTAEKIAQ
                     RGNETGAAPSPPPMPEADLSIQPMELKAGQPVTLTVTVTNRGKGPLYRFQARSKSDDP
                     ALNGLLFYMGKVDPGQSVADAVTLPLPPDRPDGTIPLSLEFEEYNGFTPAPLQAALAL
                     TGQPRPRFAFTHQLLDDGSGQSVGNSDGRIQTGEAIDILVTIKNVGPVLAQHTTIDLT
                     VPQSRDFRLSQSTLDFGPLKPEETKIARVNLFVARDTKADELPLRLFIRERGMNVTLD
                     GTLKLAIDHRPAPQIIATNKLVSVHDASAKIHSGAGEETSVIASAAKDQGLAVTGELG
                     DWWRVQISPKETGWIAKRDAREPVERVKGDMPIPSIAGAPVVKLFQNAPPVIALASPS
                     DGADVTVDRILLAGAAASERGIARVEIRVNGHLLTQREARGIAVAPDAPAAQPNLEFS
                     ERLTLKEGKNEIVVSAIDRDNLSAQKVITVIRQVDRAKVWAVVIGISKYKTVHPLRYA
                     DVDAQAFHDYLMRNVGVPPENATLLLNEQATLMNLKRTLGTELKRKAAEKDTVIIYYA
                     GHGAPEADASASDDDGLEKYIVPYDADPKDLYSTGLPMREVETIFNRLAPERVIFISD
                     SCYSGAAAGRTFATASRRAVVSDTFLSRLSKGKGRIVLSASKASEVSEEREDLGHGVF
                     TYYLLEGLRGNADTDRDGVITVDEAYGYVSKKVPEVTGQNQHPVKKGEVEGQLILGQV
                     R"
     gene            complement(33299..33595)
                     /locus_tag="PK589_09340"
     CDS             complement(33299..33595)
                     /locus_tag="PK589_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19846.1"
                     /translation="MLSLILTLAFLILPYPLPVHVVLPATSAEAALLGGTIVSINQEA
                     LSLTILFPTGESRALSVRDARLLHGLSVGDHVTFELNDEHTLIKIAKLPTDPAN"
     gene            33730..34950
                     /locus_tag="PK589_09345"
     CDS             33730..34950
                     /locus_tag="PK589_09345"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HNJ19847.1"
                     /translation="MPPSKSDVPALLTVNAGSSSIKWALFHLGASPVRTASGRIERIG
                     IPDGIVTVTDRATGQDQRHIAQIPNHAAAVQILIDQLGQSGRGSSLQAIGHRVVHGGS
                     RYTNPEVISTAVMEELRRLSPYDPEHLPAELTIIDALRAQYPTVPQVACFDTGFHQHL
                     PAVARLLAIPRRYEKQGVRRYGFHGLSYAYLMEELERVAGHEAAQGKVVLAHLGNGAS
                     LAAVRDGVSIDTTMGFTPASGVPMSTRSGDLDPGLVSYLSQTEGMTAEEFHHMVHAQS
                     GLLGLSELSPDLRDLLAQERHDTRAAEAVAVFCYQGKKWIGAYAAALGGLDQLVFSGG
                     IGEHSSVVRARMCDGLAFLGIQLDQARNDAHEAVISAPGSRVTVRVIHTDEERHIAQS
                     VCRLLDLPLSGGTK"
     gene            35014..37389
                     /locus_tag="PK589_09350"
     CDS             35014..37389
                     /locus_tag="PK589_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HNJ19848.1"
                     /translation="MTQTTHTNQPLSAEMVQRIHAYWRAANYLSVGQIYLYDNPLLKQ
                     PLKREHIKPRLLGHWGTTPGLNFIYAHLNRIIKAQNLPVLYIAGPGHGGPGLVANVYL
                     EGTYSEVYPNISQDEAGLQRLFKQFSFPGGIPSHVAPETPGSIHEGGELGYSLAHAYG
                     AAFDHPELLVACVIGDGEAETGPLAGSWHANKFLNPVRDGAVLPILHLNGYKIAGPTV
                     LARMPPDELESLLIGYGHQPFFVEGDDPETMHQLMASTLDEIVATIRRIQQEARSHGF
                     TRRPRWPMIVLRTPKGWTGPKEVDGKLTEGTFRSHQVPMAEMDKPGHVELLERWLKSY
                     KPEELFDDTGKLMPELASLAPTGERRMGAIACANGGIILRDLHMPDFRQYAVPVVKPG
                     AEEAEATRVQGLFLRDVLKLNMRNFRIFGPDETASNRWGAVFEVTDRCSTEEILPTDE
                     HVSHDGRVMEILSEHLCQGWLEGYLLTGRHGFMNCYEAFIHIVDSMFNQHAKWLKTSR
                     EIPWRRPIASLNYLLTSHVWRQDHNGFSHQDPGFIDHVVNKKAEVIRVYLPPDANTLL
                     SVTDHCLRSRDYVNVVVAGKQPAPQWLDMESAILHCTAGIGIWEWASNDRGSDPDVVM
                     ACCGDVPTMETLAAVEILRVAQPNLKVRVVNVVDLMKLQPASEHPHGMSDKEFDALFT
                     TDKPIIFAFHGYPWLIHRLTYRRTNHHNLHVRGYKEEGTTTTPFDMVVLNDLDRFHLV
                     ADVVDRVPQLGARAAYLKQEMRDKRMQHKQYIEIHGTDMPEIRNWKWSGKR"
     gene            37393..38079
                     /locus_tag="PK589_09355"
     CDS             37393..38079
                     /locus_tag="PK589_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNJ19849.1"
                     /translation="MAVPRVVALFDVDNTLMDNDRITADLTQHLDEEIGARGERQYWA
                     IFEELREKLGYVDYLGALQRYRQEYPRDPHLLALSRFLLNYPFTERVFPQVHEVIRQT
                     AQWAKPVILSDGDVVFQPHKVDRSGLFDAVQGHVLIYIHKEQELDDVEQRYPADHYVL
                     IDDKLRILTAVKARWGARVTTVFPRQGHYAKDPEVLRTYPAADLSIDRIGDLLTLDRS
                     ALLAAAKPRT"
     gene            38134..39195
                     /locus_tag="PK589_09360"
     CDS             38134..39195
                     /locus_tag="PK589_09360"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ19850.1"
                     /translation="MTPKTPVNRIWSLMLGRRQLLKGLGVAGSFMALGGPGRLAEALA
                     ADSPATGTSSGDIPRRALGKTGVQVSALCFGGAHWGRIQDDGEAIRLLHAAIDAGVNF
                     FDNAWEYNGGRSEELMGKALQGKRQQVVLMTKVCSHGRDKKVALQQLDESLRRLKTDY
                     LDLWQIHEVVYEDDPDRHFAPNGATEALLEAKRQGKVRFIGFTGHKHPDIHLKMLSHD
                     FPFDTCQMPLNVFDGTYRSFEREVLPVLNQRGIAPLGMKSLTGNAEPIKQGIVQPEEA
                     IRYVLSLPIASLVSGIDSPQVLKQNLDIARRFTPMTIAEMEGLRARVAMFAMDGRYEL
                     FKSTNRYDGPIGRQQHGIS"
     gene            complement(39282..40868)
                     /locus_tag="PK589_09365"
     CDS             complement(39282..40868)
                     /locus_tag="PK589_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19851.1"
                     /translation="MAHSFSDEQILQQSIRRRWGAIDWDLHFPWWRRPAKVRILMYAD
                     GSVHFNGGSFLGLQYVYNLLKTRAYSYVDFAIDFAHRTGGDPSATIAGARQLTDPALD
                     IMNKYDEIWFFGIESTPNLTAAEVALLTQFMAGPKFGGVLVTGDHDNLGRGISGQIPR
                     AGVMRRYPAPPDYGPTWNTTLEDGPDAGSTFDFDDQSDDTPQSIRWKRYTIASPLLLT
                     RRYRPHPVLCGPDGPINFLPDHQHEGEALAPTPVSGDPAWPTKGGHQETPEVIAWGRI
                     KDPNATKHGQEIGVISAYNGHNVDVGRILADSTWHHWFDINLTGRFAPPSPYSGFDET
                     PAGQLALKKIDAYFLNCGVWLAPPARQIEMRNAAWWSILWTDRMVELSPAASLTRFGE
                     EAIDALGGRASRCTVSEFIFKEPIFKEKIPRHEWPMLFDRFQLIDLPFERYVAGGILR
                     QLLHEVGPHNPKLTFPGKPAADEVLDKAIRTGTEEGLMALSKDLRREARMAVRLADSG
                     FLASALEDRVQRVTEETVTA"
     gene            complement(41270..42703)
                     /locus_tag="PK589_09370"
     CDS             complement(41270..42703)
                     /locus_tag="PK589_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HNJ19852.1"
                     /translation="MSLSQDQPLTPAADNGQRNLVGPVNHADRTESPSNPSGRSSALN
                     WETREGRSYLIPAAEILAYLFLLNQFDRHFTEPMSDYRTTGSTIRQHLTDSKWVLDND
                     QFSVNQFLHPYSGSIYFGLARSSGLNFWESSLYSVAGSFLWEIGGEKTNPSINDMIAT
                     PIGGTFLGEPLFRMANLLLESDDGRPGFWRELGAAIISPPTGFNRLVFGNRFDAVYPS
                     NRPATFIRLAGGGTLTSSSHNVASNVNEHGAVGEFTLTYGLPGKPGYSYARPFDYFDF
                     HVSAATANTLETITSRGLLAGKTYASGDTTRGIWGLFGSYDYISPQVFRVSSTALSLG
                     TVWQSWLADELALQGTFLGGAGYGAAGSIQRTEERDYHYGTTPQALLALRLIYANRAM
                     LDITGREYYVSDFLSPERHGQENIVRAESSLTLRVFDRHGVALRYSLSHRDAHYPSVE
                     FRNQTVTAVSLMYVILGDSGFGAVEWR"
     gene            complement(42891..43790)
                     /locus_tag="PK589_09375"
     CDS             complement(42891..43790)
                     /locus_tag="PK589_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNJ19853.1"
                     /translation="MAPRVTHRAGTYSPHRRRSRRRLTFTSLWRFTGRRWRDLRAFLR
                     AWGNTHPLPRLLIGIILIACLWGTLNWTYHAINKPTEVLFPLEDALDKNPAATWQEYG
                     PLFREHSTAIITPDLLAALAQVEGSGNPVARTYWRWRLSWNPFDWYRPASSAVGMFQL
                     TDGTFREAKRYCIHNHVVVEDGPWNDMHACWFNSFYTRIVPSHAIELTAALLDRQVAA
                     AIGSRRSASISRQQKQDLAAVIHLCGAGAGHAFVARGFHLAPHQKCGDHHASSYLARV
                     NGLKVQFARLAAGSSLQRLIKPR"
     gene            complement(43903..44685)
                     /locus_tag="PK589_09380"
     CDS             complement(43903..44685)
                     /locus_tag="PK589_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19854.1"
                     /translation="MIRVDITGEVEPQDVIQAIAALRENDQARQELLALRAETDRLQA
                     QVDDTNRALAAASTPYDVQQYTDQRQDMLDQLQANALLSQAWTSWTYPTLGFYSYPWI
                     GVSGINGLLLQAQRLSPHHRHLPLAQRTIMANLSAPPASPPVSTAPRASLLVPPVPHV
                     HHQQAAPLLNSQGQQAKVGDVVVIPTPRSIPSTPQYTPHAPPYQVHPNHFWRPTPPNV
                     QSLSSVPPQHSPSVSMAPRSSGGYAGHSGGGHSHSSGGHHGR"
     gene            complement(44689..45189)
                     /locus_tag="PK589_09385"
     CDS             complement(44689..45189)
                     /locus_tag="PK589_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19855.1"
                     /translation="MFFSRSTPTTAALLVLWLSVLPTAPSFASTTIYSYTNDEGVQTF
                     TNELASVPEKYRPQLTQRSFDTPARPETPAPDMASELRPRSAEARTITARGEYRMGDH
                     DNRADAVRLATEAAKQDALEQVATYVERITEVKDLNVTRDDIRSFTAGVVRVIDQTVT
                     TRLEQD"
     gene            45392..46060
                     /locus_tag="PK589_09390"
     CDS             45392..46060
                     /locus_tag="PK589_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19856.1"
                     /translation="MLVVKRIIAVVFGLVPLVLIAAGVAMVFRELRPTDPLLYSVQQD
                     GELPEAFMFAAVGCAGLFGCHRLWRADAGWKWTLMPIAVMVAAIAIPNLLYGHHGSPS
                     DRAYSNMFLRLGTFAVSSKQLAQEQGQFTCDPSVKLFGLSRFTQNGQPLPYVIHCVLD
                     ATGLALATPPERPGTLVFAVSPDRKQAWFAATVLARRTDRHATWLRQQGQPRVIAFSV
                     QEKN"
     gene            46214..47545
                     /locus_tag="PK589_09395"
     CDS             46214..47545
                     /locus_tag="PK589_09395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNJ19857.1"
                     /translation="MSQPSPSVPSNLFLLCISGPDLGKRIALRDGELLIGRSLQCDVL
                     SDDQDVVERHAVVLWENGQLICRAVQGIVYLDGHKTMESVLTPQQQIRMGRSLWQLNG
                     PTPGRGSGGWIEDLSDRISEVAGVERLQGFNARDMFSDVLRKRTDEEIEEHFTAGTLT
                     TTPPIEKVITGWPKPWVFFKTFILSAVVYLAFVFAMKEFNNANLVPGLIMTGAFLIPS
                     SLLIFFFEMNVLRNVSLYQVVKMVMFGGVTSLILSLFLFKWTDLDSWMGAMSAGVIEE
                     TGKALALLLIINNQKYRTALNGLLFGAAVGTGFSAFESAGYALMAGLSGGQSAMLHSI
                     TVRGLLSMLGLHIVWTSMVGAALWRIRGNRSFTIEMLYDKRFLRIFGLAVALHMFWNA
                     PFNLPFYLKYIALGFIAWVVNLALVQVGLREVKALQEAQASEASDSRLRTA"
     gene            complement(47577..>47973)
                     /locus_tag="PK589_09400"
     CDS             complement(47577..>47973)
                     /locus_tag="PK589_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19858.1"
                     /translation="AKDEGRMPFSCLRSLTVQAAIGSTKTAPLVRLALVTDEREIPLS
                     RAYAGGMRREYEELAITIRRLMQMNAERTNFLLDSLTAALAQGRKLDAIKLVRMTKDM
                     TLEEARQFVEQLAVSPTAGSRSNDSPPSS"
BASE COUNT         9256 a        13547 c        14862 g        10208 t
ORIGIN      
        1 tggcagggta ctcgtgcggt gacggagcct gagaatacgg tgccgaatcc gcggtatcaa
       61 tcggcccatc ccggcgaacg gttgccgatt gtgttcgagc cgttgaccgg gaaggtctcc
      121 tggccgcact tgaagccgca tttcggtcgc cgcgtgccgt tctcgaagag ccacaatccc
      181 tcgccctggc ttgagatgat ccatctcgac gaggacggcc agccgagttc ctatcccgcc
      241 aagccgggtg agaacggacg ctggagcctg tgcccggaga atgccggctc gaagaaatac
      301 aacgtgcact tcatccatac gccgatgatg ctcgccgaca agcaaggcga caccgcggcc
      361 atcatggatg aaggcgggtt gatctatgtg ttgcatgaag aggaagcggc cattcggaag
      421 aacaacgacc tgaagttccc attggtcgtg cgctccaaca tctatgattg cgtcgattgg
      481 atgttgacca gcgaatggga tgacgacgac ttcggcaatt tccacgcgtc gaaggtcaac
      541 acccattggc acttcctgca gttcgacaac cagtcgtcgg acggcgtgat caccggcttc
      601 tcgaacgaac agtcggtgcg cccgttcacc atgctggaaa aaaagacgga caagggcttg
      661 cccttgccct tgaacaccac gtttacgaag gcggggaaga agggcgatcg cgtgatcacc
      721 ctgaagaacg ccgcgcagta tcatccgggc atcgaaattc tcatcggcgc ggataacgtc
      781 ggtgggaatg aagtcggacg gatccagtcg atcaagggca acgagatcac gctcgtccgg
      841 ccgttgaaga acgatcatcc tgtgaaggat atcgtgacgg tggaatttgt ccggcagcgg
      901 ttctgggtag attccgatgt gggcaccgtg ttctggcacg accatgcgtt gggccggatc
      961 acctggggcc acggcggatt cggtacgatg ctggttgagc cggtcggatc gacctatcac
     1021 aatccgaaga ccggtgcgcc gatccggagc ggtccgttgg cggacatccg caccgcggag
     1081 cctgtgggct acggggtgaa cggcagcttc cgtgagttgt tggtgcagtt gaacgatagc
     1141 gtgccgcaca cgatcaacat cgtgacggcg ggtaatcctc cggggcagcc ggtggaagtg
     1201 gccctggaag cggggaagac gatttcgttc ccgattccag accatatcaa gatgacgccg
     1261 atgccgttcc tgaacggcgg tacccacacg acgggcggcg gtctcaactt cagagcggaa
     1321 ccgatcagcg gacgcctgaa ggcgaatccg gatacgtcga agctcttcag cagcgccgta
     1381 cacggtgatc cgtacacccc gatggtgcga gcctacctgg gggatacggt cgtgttccgg
     1441 ttgttgcaga cgatgaccaa tgaaagcatg gtctggacgt tgtcgggtca tacctatctg
     1501 accgagcggt atgccggcga tgccaaccgg aagaactcga tccatgtcgg tatcgctgaa
     1561 cgatacgacc tggtggtgcc gcaagcaggc ggaccacgct tgcaggcggg tgactacatc
     1621 cacttcaacg gccgttcctc caagttctcg gaaggcgctt ggggtatctt gcgcgtcctc
     1681 gataaggacg tcacggatct ccagaagttg cctgccggat acagtggccg gaacgaaatt
     1741 ccgaaggccc cggcggtatg tcctgccgat gcgccggtga agagtttcaa tgtgtcggcg
     1801 atcgatttcc cgtcgatgaa gttgaacccg aaggctccgg atgccatcga ggtcgacttc
     1861 gagcggacca tccagatggt caatcccgaa gccaagatct atgtcttgga tgaggatgtg
     1921 gcgacggtgg cgtccggcct gcagccgatg ccgctgacgt tgcgcgcgaa cgtcggggac
     1981 tgtctcaagg ttaagttgag caacaagatg aagcagggcc gtgcgtcgtt ctcggcgctg
     2041 ggtctggctt ttgatccgaa ggattccctg ggtgccaacg tgggcaacaa tccgggcgag
     2101 caaacggtcg ctccgggtga gagccgcgtg tacacgttct atgccgatcc gttcatcgga
     2161 gagactgcgt ctctggtgtg ggattgggga aatgtcatga ccaatccgcg caacgggttg
     2221 tatggcggta tcgtgatcgg ccccaaggga gcgacctatc gcgatccgaa gaccggtgcg
     2281 gatatctcca ccaagaacag ctggattgcc gatgtcattg tggatcggac gatccagggc
     2341 tacgaaactc gccagaacta tcgcgatgtg gccctgttct tccaggatga ggacaacatc
     2401 attggaacga gcttcatgcc ctacgtgcaa aacacggcgg gtttgacggc cgtgaactac
     2461 cgtgcggaac cgtataagtt ccgtgaagcc agcggctgct cgctcggcaa ggtgttccag
     2521 ccctgctcgg tggacaaacc ggaaagcatt gccactccga tcatcgaagc gcatgcgggc
     2581 gatcccgtgc gcatccatgt cttcggtgcc agcaatgaac agaacggcat gttcagtgtg
     2641 gaaaaacatg aatggccgat cgagccgttc atgcgcggcg ccgaccagat cagcgtcgtg
     2701 gagttctcgg cgtcagaatc gctggatgcc ttcatccaag cggcaggcgg aagcttccgt
     2761 cagccgggcg attatgtgtg gagcaatcag cgcttgccct atgcccaatc gggtcaatgg
     2821 ggtctcttga aggtgttgcc caccgatgac caacgaatcc tcccgttgag tcagcaggca
     2881 ccttctgtca agcgggcggg atctgagtcc ggcgaggcgc acgcctcgaa gacttcacat
     2941 ctcatccaat aaggtgaggg ggcggcggcc tcttctatcg aaggggccgc cgtaaggatg
     3001 cagtgccggt cctaatcacc aagggggaag ccgaaaggct tccccctttt tatttgtcat
     3061 aaattggtgt ccgatctgtg ttgcgtcagc attcagctca aaacgacagt tggggcgggc
     3121 tgatgtttgc gcttgtactc ggtaacttct acaatcctcc ctgtcgcaca acagatcgtt
     3181 cctgtcacgg ctttcatccc ggctcgaagc gagggcttgg tatgtcagca cttctcatcc
     3241 ttcttttgac gatcgcgggc tggtccttta ccgctcaggc ctgggccttt ggagatctgg
     3301 gccatcgaac ggtcggagcc attgccgatg cccagctcac acctgaaacg agggcggcag
     3361 tttggaccct ccttcgttcg ggcgaaacct tggcgacggt cgcaacctgg ccggacgagg
     3421 tgaaacgcgc caagaaaaat ctcgggccat tggcccatga cgccgaggct cagcgtttca
     3481 acgaagactt ccccgacaat cgggaatggc attatgtcga tcttccgctg gataccgccg
     3541 tctatacgga aggagcggtc ggaagccgcc tcaacgacat tgtgcagacg attcagcgct
     3601 gcatccgtgt cattgaatca cagacggttt ctgatcacga gatgaccagg gcgcaggcct
     3661 tgcgctggct ggttcattta ctcggcgacc ttcaccagcc gctccacctc ggcagtggtt
     3721 attatcagtt cgatgctcaa cgtcaggccc acctcatcac cgatccccat gaagcggcgg
     3781 ggaaggacga agaccgtggc ggcaccctgt tattaactgc agccgaagag cagctacacg
     3841 ccttctggga tgaccgtctg gtgaacgagg tagccaaagg ccggtcgtat ctggaactcg
     3901 ctcaggatct tctggcacaa ggccatcaag gagcctggga cggcgacggc tcgtatcatc
     3961 actgggttga tcagtgggcg gttgagggca tcaggctagg aaagtcggct tatgagcgcc
     4021 tcgtgttcgg cacggcgcaa ttcgacggag accatactgg tctgcggaag attacgatca
     4081 ccctgccccg gggctacgaa caagatgagg cgaaaattgt ggagcagcag ttagccaagg
     4141 ctggctatcg tcttgccgcg atcttgaatg ccctgcggtt cccggccgca gcgtcacatt
     4201 gagccggcct gcttcatatt gttttgaacg acgccgtggg tctcctccca aatcctcaaa
     4261 gatcagagtg cgagtgacgc agcgaacagg agaactcgct actcccttct gtgaggaaga
     4321 gttcccatcc atcaatccat tgaaacgtat cgaagaggca ggatttaagg tgcgacctct
     4381 atagtcaacg aggtggcggc ccagtctgcc gccggtgtga gggcggatgt ctgcgcggtg
     4441 gatggagcga tgggtggtga gaggttttga ctcacaggcg gctgaggcga ggaaggtcca
     4501 gccgtcggag ccgttggcgt ctgggttggc ggcgtcgaag gttggttcgt caacgccgct
     4561 ggagtcggat catagacgga tgaaagtccc cgcgtcgcga ttcgactcaa gtcctgccga
     4621 aggtgtgtca tgctagacga gagaatcgat cgagtggtca ccatcgttgt ggcgagaggg
     4681 ccggggggag tggtggctat gacgcgttgg cggatggcct gcgcggtctc gagatctgtc
     4741 gacgcaaaca cacgcaccgt atcagtgccg gccggcggcg catagtccca atggaatccg
     4801 gcatggttgc ccgactgtaa cgagtcggga atggtgacca cgtcaccggc ccgcacccgg
     4861 ccttccgggt agaatgatgg ctgctggtgc actgtgggaa acaacagatt cagatgacct
     4921 tccgaatcca cgtcgacaat ggtcagataa ctgtcagcgg tcgcctggat ttccaactgc
     4981 aggctgttgc ctggtgtgcg gggttcccct tcatgccgga tgcgatactg tgcgggctgt
     5041 gtgtcggcga cgacggcaat gccgcgcgct tgacggcgtg ctgccccggc aatccgcacg
     5101 tccagtttga tttgtgccga cggattgtcg agcgtcagga gttcggaggc gttcgctgat
     5161 cgggagagga cccgtgccag tccggcgccc cactgtgcgt tgtccagtga gaacgagctg
     5221 acgagttgaa gcccgtcggc cgaatacaac tgcaaccgat tgcctttcac ctccaccaga
     5281 aattgcgcag gctggcccgg cccgacgatc tggacctcac gtccctgttc acgcaggact
     5341 tgctcaattt tttcacggcg gtcatttgga atttccaaca gacgaacggg cacgccctct
     5401 acggaggccg gcggcagaaa ttcgatggcg cgtgagccgg tcgggatggt gaaggctgtc
     5461 gcgtgcggtg cggtaacggc gtctttcccg cgggattgtg tcaccgtgag tagcccgagc
     5521 gattttcccg gtgcgaaatt ggtttcgttc ggtggatata cggcccaagt ggatcccggt
     5581 gcagcaccca gctggagacc gttcaacagg agggtctcgc ctcccttgtg agccttcacc
     5641 tcgacccagg gcaaccgcgc cgttccattt gtaccagtct cattgccagt ctccgaaggc
     5701 atgggcagca ggggcgcgtc catgagcgcc ggcggcgcct ccaactgggg ttccggcatc
     5761 gagatccggc cgaagtgagt ttggatccgc ttcatctccc gttcgacgcc gaagaaaatc
     5821 tctctcggcg aggctgatag cccggccgat cccaaactgc gagacagcga ataggagaag
     5881 aacccgtggt aacgtccacc caccgggcca tcgagggcct cctggttggc ggcggcgccg
     5941 gtcatgagga cgtagcgggc agccgtgacc ggcacaacct gacgagtgtg aaccccggac
     6001 ggtcggtaga gttctatccg ggtatcttgt ggaaccgatc gcgtccggat gtcgagggaa
     6061 cgcgttgccg tgccggaatg gcaggagtcg aggacgatca agatgctgcg ggccttcagc
     6121 ttggagacga tggcttcgag ttcatcatcg gtgatatcgg ggacgctcgg agtccgcccg
     6181 tcgtgcggga cgagggtttc atcgagatga tcctcggttt catcgccgtt gaggtcctcg
     6241 acctgggatc cgtggcccga gtagtggaca tagaccacgt ctgatggacc ggtgtcctgc
     6301 acgagtcgtt caagcgcagc gagaatgccg gatcgagtgg cagcctggtc cgtgagcatc
     6361 gtgatgtgtt gagactgaaa tccccaccgt gtggtgagca cctgccgcat ggtgtcgata
     6421 tcgttgagtg agccgttcaa ggtcggtagc acttgatacc gattgatgcc gatcagcaag
     6481 gctcgcttgg ttcccgacga tggcggctcg gtcgtaaaac tcggcaccgg ctgaacgacg
     6541 agcagggtga tgacggcgag actcagaatg gtgcgagtgg agaaacggtg cgtcatagaa
     6601 ggccctcggg ctgcaatggg tgagtcgcgc accctttaaa aaggttcatg gcgcaaggcg
     6661 ctccgcacgt cgaatcacag cggattgctg ttgcagggta tgaagctgct ggtcgagggc
     6721 agcggtttcc gtcgaaggaa tctcgatggt gtaacgtccg tctacggttg gcccatcgac
     6781 gagtcggcct ttcaacgcca ggatggtcgc gcggatgcgg gcctccggga tcgagtcctg
     6841 aaagactaga acgatcttgc tggacaccgg ctggattccg cgagacgcaa tgtggcttgg
     6901 cggcaaggcg gtctgttcac cggcggtcca cagcaggagg gtcagttggc caatcaggag
     6961 tatcgccgcc agcgcaggga tccagcgcgg ggcgaagagg cggcggaacc actgttccac
     7021 tgattcggcg gcaccttctt tagacgtggt gggtcccgct gagtgagctg gcagtgtttc
     7081 ggcctgaatg cggatcatca cctgttgctg cacgcgtagc tccggcgtcg tctcttccgc
     7141 aaaggcggtt ttcaccggtg tacgcaggtg tgtgaggtgg gccagttcgg cgcgacagtc
     7201 ggcgcagctc gccagatgtt catcgactgc ctgccgctcg gattctttca atgtccccgt
     7261 caggtaccag gggagcaatg tggccgtcgg gtgcgggggg gccagattgt gaggcgtcgt
     7321 catacgacag actccctcga gagcatccga tccatatgcc gtttcaactg ttgtttcgcg
     7381 taaaacaccc gtgtcttgac cgtattttcg gggatgctga gcagcagcgc aatctcttcg
     7441 tagggcagtt cctcataaaa gaccaggcgc aaaatttctt gatgttcggc tgacagtttt
     7501 gagagcgcct ggcgggtcac gattccgagt tgttcgcgtt ggatcccgtc aataggagtt
     7561 tcctgctgac tcggaagatc cggcacgtcg tcgagcgggg cggtggagtg atcaatcctg
     7621 ccgtggcgtc tgaccgcgtc gagggcctta tggcgggcga tgccgaaaat ccatgtcgaa
     7681 acccgggatt ggtgcgtgaa ggtgcaggcg cctcgccata atgcgaacat cacttctccg
     7741 acgatctctt cagccagagc ggcatctctg atgaaggtct ggatgtagtg atagacgcgc
     7801 ttgtcgtaga gccggtacag ggcctcgaac gcttgcgtgt ctccggtcgc cactcgcgcc
     7861 accagcgtac gttccagtgc gttatgcccg tgagtcgcgt ccatgagcct gtatgttcga
     7921 agaaatgtgc cagcggtcct gatcggcgct tctgctgtag cacagaatcc tggatcccct
     7981 caatagcaat gccggttgac ccttcgataa atgagagact acaatcgtga acctttccgg
     8041 agatggcgcg acagacgggt acgaggtgat cgcatgcata cacgcctaca gccgctccgg
     8101 accgcgtcgg gcacccgcac aagtttgctc attttcctcc cgtttctcct gctcgcttcc
     8161 tgcgcggccg caccctttct gattcccgcc ggcattgaat tcgcgcgcaa cctgctggtc
     8221 accagcaaca agaattacgg tgggaaatac tcagaagaca tgaaccggct catggtacgc
     8281 ctgagcacac cctacgtggc gatgggattg cccatgggaa cgtcacctgc ggcgttagtc
     8341 ccgccgacca tgttgatgca gcagcaacag gcgatgatga acgcgcaggc atcgggtcag
     8401 gcagggatgg gcatgggaat gggctatccg gatccctaca atccggctgg cgccatgatg
     8461 ggcgggtatg gcgcgcaagg gtacggcggg atgggcggat acggcggtat gagtgggtat
     8521 ggtggaatgg gcgggtatgg cacctcacag tacggcatgt cgcaatatgg ctatcaaggt
     8581 caagccgggg ggtactatga ccccaataat ccctacgcgt ccgtaccggg gatgaacgtg
     8641 caggccccgg cctatggcat gccccaaccc tacggcggcc agtatggtgc gcagtcgtat
     8701 ggcgggcaga tgggcggata cccgcctcag cccggctatc cggctcagca gggattgccg
     8761 ccgcaacctg gctatccttc cgccgcgccc ggcatggctg cgcaggcccc ggctcccggc
     8821 taccctccac aaggcatgca ggggtacggt cagcaaccga tgcagccggg gatgtatcaa
     8881 cagccgatgg atctctatca gcaacaagcc atgcaagggt atcagcagca accgatgcag
     8941 caggcggcga tgggcggcta tcagcaaccg gcggtacaag ggtatcagca gccggtgcaa
     9001 ggctatgccc agcccggcat ggcgcagccc tatgggcagc cccccaatgt cggcgtgtcg
     9061 tatccagccg gcgtgtatcc gagaggtatc ccgggaccgg ctgaaccggt ggcgttggac
     9121 gttgcgctga tccgtcaggt caatggatcg aatggcaaac aggttgccct catgcaggac
     9181 ggtgagacgc tgaagggtgg gcaggatggc gaccgcttca agctggtggt gcggacgaac
     9241 tgcgagtgtt tcgtgtacgt catctctatc gatgggtccg ggtgggcaca gtcagttttc
     9301 cctgtggcca acggcccggt gaataatcca ctcaagccgg aagtggaaca ggcctttccc
     9361 gacggaccct attggttcac gctcgatcag tttaagggcg tcgaaacctt cttcctcgtc
     9421 gcctctcctg cgcgacggac ggatctggag gagagtctag ggcaactggc cggtcaacaa
     9481 cgtccggccg gtcaggtcgc cgcgcaggtt gaggagccgg ccgtcatccc gaacggattc
     9541 ggcaaaaccc agtcgggaca ggcggctcag gtgctggacg acgggcagca ggctatgcag
     9601 gtgacgccac tgtcctatgt cgcggcgaag gtcggcgatg atgtgcgggt gacacgctgg
     9661 ttccgccacc agtagcagga tgctgaacat ggccgccagc tgggtcctcg gttctgtcag
     9721 cgactcgacg taccaggcga tgtacgcctc gccgcttcca tccctgcggc gttgctggac
     9781 aaccacgttg agcatcctgc gaattcccca tgtcgatgta gttcctggaa gccggtcatg
     9841 agcgaacaga cgacgattgt tgaacaacac aggatgcgaa cctcttctcg gaacgaatga
     9901 tcaccttcct gccgttcagg tgtcagacct ggttgtggtt gctcgctttg ctgatgacgc
     9961 tgcatggggt cgtcccgcag gcagaagccc aggtctttcc accccgctct cacacccagt
    10021 ctgtgccggc gcagccagct cctccagcga tgggccagcc gatgccgggn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ccagtctgtg ccggcgcagc cagctcctcc
    10201 agcgatgggc cagccgatgc cggggccacg gcaacagcaa ccggtgatgc ccggtcccgg
    10261 tctgcagccg atgatgcagc cgccacaggt gacgtcgcct ttggtcaaga cgattcccct
    10321 ggccctcctg ctgggcggag acctgtctaa atcttttgtt cagctcacca accttcgcgg
    10381 gcaggtcgat tcgaacttta cgatcggccg cagcctggct ggtcggttca ccgttccgcc
    10441 ctatgtgcac gaagcctcaa acggctcccg ggtgtttgtg cacgcgaacg gcctgtccaa
    10501 cccgccggtg gtgaagtcgg aaggcaatga attgcatctg gagtttcatc ttccagccct
    10561 gcagttcaaa acctattatc gggactattc tccggaaggc gaccaggcgg tcggcgatac
    10621 ggtcggcgag aatgcaatta tcgacatctt cattttaccc acgaccaatc agcagggatt
    10681 cccgacctac caggcggtac gggcggggtt tcgggggacg ctcaagccgc aggacaagtg
    10741 cacctactgg ttcgatgtga tcttccccat caacatctgt gaggtgtcga aaaaatacct
    10801 ggatacgctc agcaatgcga ttgaaacagg ggtgcgtgag gccttgagcc atccgcagac
    10861 ccgtacgcag ttcgatcaga ttgtctggca atccctccgg agcgaccttc tcatccaagc
    10921 cggtatcaat ccgatgagtc ccgcccagat ccgtgtgatt gaagcctcct tccacggcac
    10981 cgactacgta gtgaagtacc tgcctcgata attgtctcaa catcagagaa gggggggccg
    11041 cctgcaggtt ttgacgcgac cgcccgaaag gtgtgcggga cggagtgaga atttctcact
    11101 ccgtcccgtc aacggcattc cagcggctgc tcaacaacga ggggtcactt tgcggcggcc
    11161 ttggggcgca attccttcac gacatccgac gtgagggttg aaggcaccgg cgcgtcctcg
    11221 tccgaatctt gtgccttcga ttcgacccct cgtctctgca cgccagccat accggatgcg
    11281 ccgagggtat tgccctgcaa ccccggcatc acgcggctgc cggccggttt ccggcagttg
    11341 atcacctgat tggtattgtt gtcgaccgtg caacagagca tggtctctcc accgcaaaag
    11401 aggacatggg tgtagggttt attgcccatg atgtcctgtt gcatctgatc gttacaggag
    11461 gcgccgaagt tggtgttgag atccttggta ttacagctct gccaggtgcc tttgggcctt
    11521 gcagatgcgg gagcaggaag aatcacagcg aatgtcagta gactgattgc gccgatgaat
    11581 accactcgat ccgatctcat agaggcctcc ttggtgatag gtgatgggct cgcgattcta
    11641 cgggagctac gtcagctccg taatgggtct cgttctgcat gtgcgctatt gaataatggg
    11701 cggtgacgca aggccgaaga cgacgagttg gaatttctca ttggccgtcg gcgctccgtc
    11761 ccgtctcgtg gtcagcaacg ttacgccgga tccgtctttg aaaatgccgg caaaaaaacc
    11821 gctggtggtt tgaatcgtat acgtgcagtt cccctgatcg acgaggcgaa acttttccca
    11881 ggcctgcacc tgggtcgcat cgatgtgaaa aatgggcgaa gcgcctccga cacaagggcc
    11941 gccgaagagg ccgccgcaat caggggtcca gtatttccgg tcgtactttt cgacttgtcc
    12001 gcctccgccc agtgccgtga cataacgtcc gctcgtcgtc tgaagcgcgt aggtaccatc
    12061 gccttgacgg atcaccttca gtttcatttc ggcttcactg ggatgaccat tcaggccaaa
    12121 ccaggtcttg ccttgatagg tggacgccgc gaacaattcg ctgtcggcgg ccatgatgcc
    12181 gtactcatag cccgatcccg gatcaccgca cttcacgatg cgaaactgtt cccaggcttg
    12241 gacttgcctg gcatcgctgt ggatcgcatc ttgatagcgc cccccggccc ctacggcagt
    12301 gaggaagttt cccttgctcg tcccgatgcc gtagtaggga ggcgggtagc cgcccatgta
    12361 gaccagacgg aatcgctccc actctttggg ctgtgtcgca tcagtgtgca ggacattggc
    12421 cgtcatgccg ccgccgttga cggcggtgag atagttgccg gtcgcggttt ggaggagtct
    12481 gtcgtacgga gaaggcgggg tcgcgatgcc gattctaaac ttttcccagg ctccggccga
    12541 cgtggcgccg gtgatgatgg tcggatcacc ggttcgtcct ccgccgttca cggccgtcag
    12601 gtagtaacct ttttgcgtcc tgaaggcgaa ttctccagga taggcgctgg tgaatggtgt
    12661 cgagagcgtg cccaataccg ccgcacccgc ggtgcctccc ggaaggacgc gtgaaacgcc
    12721 acgctcctgg acctcggccg attcggcaga ctgaatggcg tcctgcgtag ccggcgcatc
    12781 ttcgggaatc acaggtactt cgcgtgctgg cacatcgcgt gccgcctggt ccttgctatg
    12841 atctgtcccc gcacaaccgg ccagtcccag acacaaggcc gccgtgccca cgaatgtgac
    12901 gactgctgta tgtcgcatct cagccctcct ggtccgaaaa tgatggtgca tcctgtgaga
    12961 gtttcccggt atggcttcgc attcgcggtc ctgcagccta tcgatgatcg cgcaccgtgg
    13021 gactatcgag atagggatac catcggagcg tcttggtcat gggatattca aaataggcct
    13081 ggcaattatc gttcatcgct ttcaaattgc ccttgaccat cgcagacgcg ccggtgagga
    13141 gatgttgtgc agagggaccg cccgccacgg taaatccaca tccggccgga agacaattcc
    13201 acaacatttc cgtcgcggca ttgtcctcat cgtaaaacgt ttcggttgga cgtgaaacct
    13261 tgtgcgacca gctcacttcc tgacggctcc aggcaaacgg gctgatggtg gtgcgggatc
    13321 cggcgcgggt gctgaatcga aacttgtgat attgctgatc gggggcgtac caggtgaaga
    13381 ggatctcgct tttgccgacc gcatcgcatt tactgatcaa gtcgaacgca aatggtccga
    13441 tgctgacgtc atacaatgga tcgagcgggg cctgccagtt cacggcaaac gtttcgaagc
    13501 ctccgccggc tttgggttcc cgcagggaga tcgagatggt catggtgcgc ggcttcccgt
    13561 cgccctcttt gtcgttatac agaaaccgat tgcccgaggc ccgcacgtac gactgggtga
    13621 tgcggtcatt gccggcggca gaatagtagc caatgctgat ttctaccggc tgtgtcacag
    13681 ggaggttagg ggcgatggtg accagtgtgc tcacgctctt gtaagcgagt cgcaaggcgt
    13741 tggccaccaa ggtgaggttg gcggtcggag cctggtattg cgcctgcata aacgtttttc
    13801 cgggtgttgc ctggaacttg tccacgatgc cccgttcttg tatctcgcca gtgactggat
    13861 ggtgatcgtc ggtgatcaag tccggttcct gaatcttgat gccctcgtca ggagtggtcc
    13921 cctcagttga gttcggccgc gcttcctgtg cgggtgccga cgagggcaag gagtcttgat
    13981 tgtcctgtgc ctgacaggcc atcgacaggc cccaagccat tacgaaaacc aagcatgcag
    14041 tacagggata ccgaacgatc gtcatgcatc tactcctttg tccttgtgcc tgcagcctgc
    14101 gacgctgtct cgcatgcaac ctctacgcgg cgtcgttata acaagctctg ctcattgccc
    14161 gttggttttg aaatcctgtt gaagctgttg catacgctct ggcgtcatcc attccggagc
    14221 gggagcctct tctgcctccg ctgcggtgtt cgcgtcgacg ccacgccgct ggatgccggc
    14281 actgccgagc acgcccacgc ttccttggac tcccggcatg aacgctgagc ctgccggctt
    14341 ccggcaggtt tggatctggt tggtggtatt gtcgtaggtg cagcacaaca tttgaccgcc
    14401 gctgcagaga agggcatggg tgtagggctt gtttcccatg atgtcttgct gcatctgatc
    14461 gacacaggag ttgccgaaat tggactgaat ctgatccacg gtgcagccgt tgaccagggg
    14521 ctttcgcggt ttggcatctg acgctgcagg gagcgccagg agccccgtca ggatcatgag
    14581 cgaaacggtt gtgatcgcat aaggcttggt catggtgtac tctttctcga tcatctgaac
    14641 acgtcaatga tcgaatgtcg gtgttgtttt acttattctc attatcgtcc gtgcctggcc
    14701 gcccgtcgcc ggagggtttg tcctctatac ctcgtctcat gatgggcgcg ttcatgccgg
    14761 ttctcggaac gcgtggattg atcggtgcgg gcgatacgac gggcgcgttc tgcagcatcc
    14821 cgccgggaac tctcgtcatc ccgcctcttg ggcctttgag ggcctcgatc tgttcgcagt
    14881 cttttccgcc ctgcgcattt tgtacgcaac acatataatc gccgctggga atacattcga
    14941 cccaatggcc gggtttgtcg gggcaggtgg ccactccaat cgtgacgggc cccttgcagt
    15001 tgtccggatt cttgatcggc acggccaacg cgggagcggc ggcgacaatg actccacaca
    15061 ccagtcctgc catgaggctt ggatgagaac atagtctatg catggcggcc tccttcgttc
    15121 tgtgactcct caattcgttt agcgataaca tcaggatcgt cggtcctctt tcggcaattc
    15181 gatggttccg agtgtcagta ctctatttac cgccgttgaa atggcgtgca ttcgcagctc
    15241 tgacttcctg ccggacaggc tgcgggacaa aatggcgaca acaattggca ttctgcctgg
    15301 gtatggcagg cgcatcgggt cacaggaaac gggacctggt aactgcacag attcggcggc
    15361 agggccggca tcggaccgcg gctcatgggg atgaattcgc agtgagtact tccagacggg
    15421 caggaattgg gaaagagcac agtgaggctt tggcagtccg cctggttgaa acacttgcac
    15481 tgggtcatca tgtgcgtttc ggtatggcag agttgtggcg gcggacctgc cggaccaggg
    15541 gcaaggggcc ttcgttgaag gtcttttttc atttccccca acagccctgg tgccacgccg
    15601 cgctcctgga tctcttcacc ggacggttgg ttcacagcgg gtgcctccac tggttcagcg
    15661 gatgcagaga caggatagcc ggtcgtgagt acgaagatga gcatcaccca gccccatgtg
    15721 acgaaccgtg tcgatttcat gtccatgatt ccattctctc gctcaccttt attttccttc
    15781 tttttccgag gaagtcggtt ggtcgccgtc cactccgcgc cgcatgatgg ttccggggac
    15841 aacaggggca gggactcttc ctagccccgg ttgggctggt tgaagttgtc cttgcacccc
    15901 acctgggggg agtcgcatct gactttgctg ctcggtgccc atgcaatctc tggtcgtgcc
    15961 ctggtgtgtg ccggtcggtg tggtagttgt gcaggaatgg ccaaggcagg cttccaagct
    16021 tgattcagta aactgcacgg cccccgatgg cccgaaatgc tctttcccga gttcgtctac
    16081 ccagcggcag ctgcatttac acgaccagta agtgttcttt ttgggtttgg cccaactgat
    16141 atctgactgc aggaagagac tgagtatcgt gcatacgcct gcaccagtta ggacaaacca
    16201 gcgcccgcga ctccgcgcac gtatcgtacc tcggctctcg gtgcatgtag tgggagtgtt
    16261 cattccagcc tcctgttctc catacggatg ctcacttcag atgctaggcc tcatcagccc
    16321 tggtgctccg cgcgatcaaa taatccggtg atatgtttct gcgtcaggac acttcgtgcg
    16381 tccgtactta cagtcccacc atgaccaatt cgaacttggc gttatagcca atcgatggcg
    16441 cggcattcgg atcgctgatc ctggtcgaga tcgaggagga ccccggtcca acagccagat
    16501 aaaaaccgct gacggtctgg atggtgtagg agcaatttgg ctgctccacg atcttgaact
    16561 tttcccaggc ctgaatctgt cgtgcatctg tatgcaggtt gtcgccgctg gcgatcccgc
    16621 ctccgcctac tgcggtgaga tagttcactc cgttggaggt ttgcagcgca taggatccat
    16681 cgctttgcct gaggagcctg aatctggatc cgacttgtaa gccgctgaag gcggtcacgg
    16741 cctgactggc catccctcct ccgttgagcg cggtgaggta gctcacaaaa tcgcctttcc
    16801 ccggaatatt tcctgtgccg gtggggcgaa tggcatattg gtagccggac cccacatcgc
    16861 cgcatttcaa gaaatagaaa tactcccaag agttggcctg aaccgcatcg gtatggaacg
    16921 catcagtcga tttgccgcct ccaccgagtg cggtcaaaaa atgcccatcg taggtcttga
    16981 tcgtgaaggt cccggcaggg ctgggcccgt cgattctgaa cagcgcgata tcccgttgcg
    17041 gagtctggag ttcggtttgt accccctgtg tcgtattggg caggccgccc agtccaccga
    17101 cggcactcac gaagtatccg ccgttggttt ggatggtggt gtaatccggt tggacggtcg
    17161 cgaggcgaaa tcgctggttg ggtccgatca cgctatccag tgtgatgacg gcgtcgatgc
    17221 tgtggtgccc tccatctcgg gcggtcagag gagtgttctt caggtacgac cgtatcgcaa
    17281 aatcgcccgg taacaggccg aacgccggcg ggttcgatgc cgtgttcggt gctgacggaa
    17341 tcggggtgaa cacttgcggg gaggtgaggc cgggtcggag gggtttcggg gtgacgcccc
    17401 gctcttgcac ctcgtcggct tgtggttgac ctgtggcttc ctccgtgtgg gcgatagtgg
    17461 tagcgttgga attcgatgac ggcgaatccg cagcacaacc ggcccatccc gcgagcaacc
    17521 agccggtgac aacaatggct agataccggt tcatgcggca cctccaattt tgttcgtgag
    17581 gcactccgaa ctcttacact cctgcgacgg catcatctcc gctcatcgtt ctcaccatat
    17641 ccatcgatag cgatgctccg agccccggcc agggcgcggt gcgtatcgag cggcgcgaga
    17701 ggccagtgaa cattgatgcc caatcactcc tcaacacacg gctaaggcct gtggtttgcc
    17761 cgcgcgatca attccttcag cccgttcaag tccaatgcgc ctggaaccaa ttccgtgccc
    17821 acgatgaatc cgggcgtgcc gctgatgccg agagtgcgtg ccagggcgcg attggtgtcg
    17881 atcgcctttt gccattcggg attgcgcatg tcggcttcca gcttgatggt gtcgagcccg
    17941 acttcgccgg ccaggctcaa cagcgactct ttggtgatat cgcctttgaa ggccagcatc
    18001 gcttcgtgga atacttggtg cttgccttgc gccttcgagg ccagcgcggc tttcgccgcg
    18061 agttcagacg attcgccgag aatgggaaag tctttgtaga tgacccgcac acgcgggttt
    18121 tcctgttgca attgcgtcac ggcgccggcc gcgcgtttac agtagccgca gcgataatcg
    18181 aaaaactcca cgagcgtgat gtctccggag aggttgccgc tcacgggtga cgtgggatca
    18241 ttgagcaatt cggcctggtg ggctgccagc gccaccttct gcttcgcctt ctcctccgcc
    18301 tcgcgtttcg cttccaatgc ctggagcgat tgttcgatca cttccggatg ggcgcgaatg
    18361 tattcttcga tcacctgctc catggattgg cgtgcggccg gatccagctc tgatgaggtg
    18421 gcggatccgg ccagagtggc gtgagtgaac agcaagatga gtgcggtggt ggcaatgaga
    18481 gaatggcagc gattcatcgg ccctccttca gtctcgtcat ggtgcggtgt ctggttgtgt
    18541 ggcgcagcgc ggacggtcgg agcactcggc gatccatcct tgtgatttcg ttcatgcgcc
    18601 gtatgtcgga cgcgcaaggt gaaaggttca acggagatat aagagtggct ggagagggcg
    18661 gtgcagctgg ataatcggca gatggtggaa tcatgctgat cctcgcgaag ccacgcccgg
    18721 taagcctttg cgaccggact tcgggaagct ggagcatacg cagcaccgca gttctgggtc
    18781 aactgcgtga tggaataatg caggtatttc cttcagaacc ggcgtaacgc tttctgagcc
    18841 aacgccttga ccttttcttc atcgcccgtg gtttcctgaa tcgacagatt gatggtcgtg
    18901 ctgcccttct tcacgtacag attcacgtaa ttcagtccgt gcataccacg gtccatgaat
    18961 gcctcgtcgc ccagaccctt taccgtgacg gaattcttag agatggccct gctccgcccg
    19021 atggccccgg atggaaacac ccacacctcc aatgcatcct tgccattggc aaactcgtag
    19081 ttgcaccagg cggcatcgcc ctctttgtcg cccgttggct tgcccttcaa cgcaccgacg
    19141 gtctgttcca cttcggcgat cgtcaggagg gtgcaaggat cgacattggg aggcgccgcc
    19201 gatagtgatg ttggtgtggc cgaaacaaga acggctaagg acaggtatat gaataagacg
    19261 cgcctagaaa ttcgtgtcat gccgactcct tcacgtggca acttggaaat ccaattcggg
    19321 gaattacaac cggcctatcg ctttcctgcc aagggtcttt agttgttctt cgacgcgagg
    19381 accatttctc gtcatcagta gaaccaaggt cgcgcctttc ctgatgtaca gatccacggc
    19441 ttccggatcg accttgcccc gatccatgaa ggcttcgtcg cccagtccgc tgaccatgac
    19501 cggctgtttc gcatccttgc gcaacgcctt cgaccaggag gcttcagacg tccagatttc
    19561 gaactcattt ttctcgtcgg caaagttgta gccgcatctc accccatggc ccagtgtttc
    19621 gctggtcggg gcgccgttga gcttgccgac cacctgttcg acttctgctg gcgtcaggag
    19681 cgagcaggga tccggagggc cggccaggct tggcgtagaa atgagcgcgc tgccgaggca
    19741 gagtaatgcg tacaacaaca ggagaggacg tgagcgccgc atggtgcctc ccttcacgat
    19801 gaaaacgtgg tgtgtccagg ccaaatggag tcggtccacg atcatcgaac gactcccgga
    19861 ctgaacgcct tcggtcctgg ctggatggcc ttgttcaggg ccgtcacggt gaattccacc
    19921 ggaacagacc agcgcgtggg cttcggagac agcacctgtg cgctgacacg ataactgccc
    19981 cggccggcaa tcattgccgg acctctgcca gttccaggag caccccatcc tgtatatccg
    20041 gttggtgatg tcgcttcggc caggttgacc ggtaggttgt tgaagaccgc ccagttgccc
    20101 tgactatccc ggcgttcgag ccgcaccata aagctcgtca cctgcatccc tgctggagga
    20161 agcagtttaa tgggaatggt cgtattctcc agaaatgacg cgttggcggc ggggaggaca
    20221 atcaccggtg cgacgaccgc aggcccttgc cgggcagcgc ccaggatctg ttgattgtgt
    20281 ttttgcaacg cggcggccgc gtccgtttct gttttgagcg cggcatcgcg ctgagcgatc
    20341 agcggtggtc ggttgtaact gaagccggtg gaattaggca gggcggagcc ggagaatccg
    20401 tccctcagcc agtccagcca gaagcgggct cgagcatctt gtgcttgacc gctcgtgttg
    20461 aacgacccat tcgcgcaggc cacttttccg gccccgacag caggccccag ccagggatcg
    20521 ctcgggcagg tcacggtggt ggtagccgtt gcggagaatc cttgaggcag gtagacatgc
    20581 acctcctctc tcgccagtcc cgtgtgtgag tcgtaggaac cgcgagagtc ccaattcaca
    20641 aatccggctg gcaccgcgct acacgaggtg gtgacatgat agcttgagag ggccgccgtg
    20701 aagcttgcct cgatacgagg agggccacat tgtatcgccg gctgctccgc atgcaggaca
    20761 ccgacacccg ctactgtgag gggcgctgta atcaaccata ttccgagtcg ccaatgaggg
    20821 cgcatagatc ctccctgtat gaccgaatgg tgttcattcg cccgatggat tgcggcgttc
    20881 acgactactt ctcccgctgg gactgaggag cgggcgcatt gccttccttc ttgcttgtgc
    20941 cctcgactcc acgtggccgg acgaagaggc tgcttgcgcc gccctgggag gaagtcgtcg
    21001 ggggtcgggt ggacgacgca gcaccgcccg cggttgccgg cggcgcctgc atcaccgatg
    21061 aaccggatag gggtgtctgc tgcaatgcag gcgtgagaga tttgttcgga gcgcctggcg
    21121 tcagctgaaa cggcacggga ctgctccagg ggccgggagg agttttcatg gccgctctgg
    21181 ctcgcacttg ccagcgtccg gagtaggcat ctgtgacgat cggagccacg aggtatccct
    21241 gggtgagttt cgtggtgttt acagccatga tgctgacgaa aggataggaa tctttctgtc
    21301 ccggcggtgc atcgaggtag cgcagttcca actccgccac attggtcacg ccgaccgcag
    21361 gaggaatggc ggcaatgaaa agctgtccct gcgtgacccg ttcaccgggc accggcttgg
    21421 tgatcacgaa caaatcatca ggcacttgca gcttcttggc ctgcggattg aatggcacag
    21481 atgggggcgg agtttgtacg agtggagtgg cgagctttcc cgccggtgca ccgagccgcg
    21541 cagggatcgg ggcgcagatc agtccgagag aatcgatgaa cagcccggca cggacgcgaa
    21601 agcctgaggc gatctcgcca ggcgggcatc catgcggata cggtcctttc gtcactcgtt
    21661 cttcagaagc cgcagtggga ttgccaaaat cgaagaggcc gatcgactga gcagggccga
    21721 gtgaagcaca gtggatttca acatactgta cgggcggatt gggtgatctg accgtttcga
    21781 cataccacga ttggatagcg agggtcttgt tgccggtgcc ccaacaggtt cgctgacggt
    21841 cttggccgcc tccgctcgcg ccgaacgacg gcccgaccgt cggtaatccg aacgcctgtg
    21901 atcccctcag ccaaggcgcg caggtcggcg cgatgcgatc gacccattcg cccgtccggc
    21961 cggccaggcc cacaagatag gagccttgcg ggcattcggc ccggaagaga gagccgccgg
    22021 ggccaccgat taactccaga tcggtcgccg aggagagcga ggggaagaag cccaggctca
    22081 ggacgagcag tgcaccagac aatacgtaca agccaaggcc gttcacgtct ggccgcaacc
    22141 gcttagctga gcgagcagga catcgagcca tggatcagtt tcctatttgg acgaaggacc
    22201 cgtgcccggc gtttcttgcg aaggctggtc gccttgctcg atgcctcgtc ccagaatgcc
    22261 ggggactttc ttgaattggc ggatgacttg attggttttg ttctgtgcct ggtaggcgcc
    22321 gaaccgtcgg ttgaggctat tcaccgcgtc ttgattcatg aacgacgtga atggacgccg
    22381 cctggctcga acgatatcga caatattggg agccaacggt tcgggcctgt agccttgcgc
    22441 attgggtgcc gtacgatcca atggtgcatc aactgtaggt acgatctggt cgcactgcac
    22501 agttggattc agccaggggt cggagctgca gtgcatcgtc gttttgaagc ggccgtaggg
    22561 gcgtgagggt tcgctgatct tcggggccgg cacgagaatg tcctccgccg cctggccggt
    22621 cgtgggatcg aatgtcccga ctcctgtcca cggaaccgac accctgatcg ctagtctggt
    22681 ttgggccagg ttgcaagaac ctgagaagat atacgtgtaa cgtgtcgcga caaaggtggc
    22741 gttcttgggt tcgctgccgt aacaggtagg atcgttgggg gcgaccaggt cgcctttcgc
    22801 atgcgcttcc aacggtgaca ataccgtgag cgaaaacccg atgccggcaa tcaatgacca
    22861 gttcataatg tctccctgcg tggctaaagg tacacactta ttctgccggc gtagctgccg
    22921 aatcactggg ctcgctcttc tctcgattga ggccgcgagg ccggatgact gacgtcccgg
    22981 ctccgaatcg ctccgatgga cttttgagta acggcgaggc cggcgatggg gtctgaatcg
    23041 attggttctg ttggttcggc acgagcgtga acgacgctgg agcgctccat ggtcctgcca
    23101 ctgccccttg gccgatcatc cgcacacgca tttcccagcg gccatagttg agccgcgtga
    23161 ctttcgggtc gatgtattgg ccttgcagca gttttgccgt gtccatggcg ggaatcgtat
    23221 tgacgaaacg cggctgtcct gcgggcgtat cgagccacgc gaattccaac tccgccacgg
    23281 gctccttccc aatgagcggc ggctgaattt tgacgagcaa ctgaccgacg accaccgtcg
    23341 cgcccgacgc cggactgagg atgcggggca tctgtcgcga gagaatggca tcagaggtgt
    23401 tcggcccgcc tggagtgaaa ggagcaaccc cgggtccttg aggttttgac agggtagtct
    23461 gcgacggatc gaaggtttgt tgcgcggcca tggcgatgtg atattgcttg tcgatcgctg
    23521 cgcgctgtgc gggattgagt ttgaatgata agggcaggtt tggagcctcc tgcagcaact
    23581 gctgataggc catcgacaca tatcccggaa atgaaggtgg cgaggtcttt ctcgttacca
    23641 ggcggcaagg gccgttgggc atgatgcgcc aggggtctga gccgcattgc atggtggagc
    23701 tcacggttcc tgtggccgta gtttttccga actcgctcaa cgccaggttc atcgtttcaa
    23761 aggtcgcctt cgtattgggc tgatggcttg cctgcacggt ccagtgcacg gcgacattgt
    23821 tcacctgtgc ggtgccacct ctcgatgacg tttcaaagag gaggcattga cctgtgatcg
    23881 cataatcgga actcagtggc ttgtgcgttc cgttgattgt gttcacggag caggtcatgg
    23941 agagttggcc cacaacagct ggttcatcgg ccacggcgac ttcggtggaa accgctaagg
    24001 cgcaatgcaa cacaacgatc ccgagcactc cgacgatcca ggtcaggctc atgtttctag
    24061 ctggtgcagg tgtcatcgaa tccttcctgg tatcatgcgc cggaccgctg ccactattat
    24121 tttgcgaacg atttggtggc cggttggaga atcttgttcg tcggcggagc catgacgccg
    24181 aactccaccc agtcgctcca tccggattgt tgaggagtgg tcacttgggc gcttaaccgc
    24241 catgcgccgg ggatcgacaa gaaggccggc ggggcccccg cgccgaagcc ggtataccct
    24301 gtcgacgact ccgcttgagc ggcccccacg ggaagtgtgg catggggaac ccaaattccg
    24361 ttggggtctt ttcgctcgag ccgcaccatg tatccggtca cgctcttcgc ggtcttgacg
    24421 ggggttccgt cgagacggac ttccgtatcg gcccatggtg gagggggcgc taacttgatc
    24481 gagatgggag attggttaaa gaaccgctgt cccgcggtgg gcgcagtgat gatcggagag
    24541 agactcatcc ggtaaggcgt cagggtctga gttgccgaac gcagccgttt ctcggcctgc
    24601 gccttggcca atgccgccgc ctcggccttc aaatccgcgt cccgtttcgc cgccaaagcc
    24661 gtgcgttctg ccggcatcaa gacccaagcc gacatgggct tgtaggctct ccatgcattg
    24721 aacagatcgc tgagagttgg gagaggtttc ccatcctggt actttcgggg gtaggcctcg
    24781 gagggactat tgtccattcg gttcacgatc tgacacttca cgcgaagact ttccgggccg
    24841 aacggaggcc cgtccatcgt gagccaggga tcggatgggc atgaataggt tgtgcgccag
    24901 gtcccatatg ggtgactcgg ttgcgagatc ggagccgccg cgatttcaat cacctcctcg
    24961 gcgatgttat tggcataggc acccttcacc gtgtagggaa acgtcctcgg cgctcccagt
    25021 gatccgggtg tggtgcaagt gccggtcgag acgtattgcg ccttggtgga agagaagctg
    25081 ccgaccatct gttgtgtcgc gctgctgcag ggcgccgagc cgacgccgtt gaatccaaat
    25141 ccgaaacaca tggacgcgag ggccacgatc aggacgagcc cggctacgac agtcatggcg
    25201 tgccgatgcc aaatattcat gtggagcctc attgatgaaa ggtatcttcc tagtgcttcc
    25261 tatggcccga acgtcttcgg cgcccgttga atcgccttgt tcgtgttcat cacgacaaat
    25321 tccgcaggct ggctccagcc ggtttgttgc ggggcggaga cctgagcggt aatccgatac
    25381 gttccgggag aggccatcaa agaaaggctt cgtccatccg gcccgccggc gccccatcca
    25441 aggtacccgg aaggagacga tgcctgggcg aggccgatcg ggagggcggt gacaagagcc
    25501 caagtcccgc ctgggttctt ccgttcgatt tttactgtgt agagagtgac cgtcacccgt
    25561 cccggaggcg gcgcaagttt gattggcacg gttgtcatgc cgaggtagtg cgatccggcc
    25621 gtcggggaga ccacaaaagg ggcaaggttc tcgagcaggg tcgaccccgg ctgcaccttt
    25681 ttctgaagct gctgagcctc ttttcgagcc gcttcttgtg cgagacgcgc cgtttcggca
    25741 agggcctgtt gttccgcctg tagatctctt ggtcgttggg ccaccagagc cgcccgatcg
    25801 taagagaacc cggtggtatt aggtttcaac gtctgttcga agccgttccg caggtaggtg
    25861 acgatgtaat cgagactttg cacatctccg gtggaagaaa agaccggctg ggtgcaggcc
    25921 gctttgccca tcaccagcca gggatcttcc acgcacccaa gcctggtttc gattttcccc
    25981 cgaagcggcg ggttcccgtt taattggatc gtttctgcca ccttgccttg cccgggggca
    26041 taggcacctt ccgaagtcca tggaatcggg atcacttcca catggtcctc gtacttcagt
    26101 tgctgatcgc accgtccggt catgcgatag gtggatttcg ccgccgtgaa cttcgcttga
    26161 atggagtcca gatggcagct gaccgtcgtc atcggataga tcctcacgac gacgccagca
    26221 gcactggcat caccggcaat ggcggacagc acgaacaaca gtacgcccca catgtgtctc
    26281 tgcatgaaga tccttcctta tcctgattca aatgatggcg cgtgatcttt cttatggtca
    26341 tctctccgga gtcgcccgtc ccagtacgcg ccggcctcgt ccaagcatgg aagcaaccac
    26401 caaggatccg gggacccccc tgatccaatt gaacgagggc cggttcgcgt atctacccgg
    26461 gtgtgctcgc ttcaagagaa catacgtgca gaatgcaccg gcgagcacga acagaacgaa
    26521 ccaatccccg gtcttcgagt agacggtgcc tcctccgccg agggccacag cgcccgtgat
    26581 ggccgattcc tgaaagacgt tcgatgtggc ggcgaccacc cggccatacc catcgatgag
    26641 ttgcgatgga cctgcgttcg aggcgatcac gaccggcaca cgattttcca cggctcgcat
    26701 gacggacatg agattgtgtt gccggggggc agcggtgggg ccgaaccaga cgtcgttcgt
    26761 cagttgaacc agaagctgtg ccccgccgtt gacggattcc cgggcgaggt gtgagaagag
    26821 attttcccaa cagatcaatg cggcgacgga catctgcggc gcgatcgtaa acagctgacc
    26881 actgtcacca gcagtcagtt cggtgacgcg cggagcaagc cattcgggcc agggaatgac
    26941 atcggcctgc ggcacatatt cggcaaaggg cacgagcatg cgtttgcgat agggaggcga
    27001 ggtcgcgcca ctcggctgga atagatgagc ggtgttgaac agccgtcgtc cgatggtgag
    27061 cagattgtca cccgtggcga atttttccac ctcggctgcc ccgaggatca agggcacggc
    27121 ggtgtcgctg ctcaactgct ggaggcgatg cagtagagcc gggtcggaca catcgccggg
    27181 gatggcgctt tccggccaga cgatcaaatc cggtcgcgcc gaagccgcct ctcgcgtcaa
    27241 ccgctcaaga cgggccaggt tggcctcccg ccctgcttct gtcgtgcggt cgccgacttg
    27301 aatgttgggt tgaagagccg caaccctgat ggatcgtcct gaggcaggcg agaagagttc
    27361 ggtggcgccc caggcgtgtg ccgtgacaag gatgaggagc gcgactgtcg cgtatgttcg
    27421 ctctcgtctc aagcacaggg cggcaagggc ggcattgccc agtgccacca ggaacgtgac
    27481 gccatgttct ccaatcatgc tggctgtctg cagcagcgcg agattacgat gctgcgtctg
    27541 agcgagcgtg ccccagggga gcgctaaaaa cccggcatgt ccacggagat aatcgatgag
    27601 tagccagagg accggcgcgc acaggagaag cggaatgtct cggcgaagtg cccaggaagt
    27661 ggccgatgcc catattgccg gataggctcc gacgtacaga gctagcagca cggcatgacg
    27721 tagatcgaag ctaggcactt cgaacagcca gccatacatg ccgaagctag ccaggacgcc
    27781 ggtcagcagt cctagcgcgg ccgcccgacc agggctgagt ccctggatgg ccaggagaat
    27841 cgggacgagt gcgccccaac ccaaccagcc gatatcgggg gagggcaggg atacgacgag
    27901 tagtgccgca gatagcagcg ccagcgcgat aggtgtgatg ccgtcagtta cgcgcagccg
    27961 ttgatgtgcc gtcatcccga ctccccctgt cggcctcagg cgctccattc agatagcgac
    28021 tattccccat accgtcggac cggctctgat cgactcaacc gcccgaggta agtctgccga
    28081 tcgtcggcac aggttcattc tattattgca ccggcgccga cggcggaatg agcgtggtgg
    28141 tccaggcctt cgccgccttc gcatcgctct ccgcgggctt cgtgcatttc ttgccgcgca
    28201 cgccgtaact gatcgtgttg gtccggatcg gacaggttcc gaaggcggtg ccgaattgga
    28261 cgacgccttc gatcaccgtc agaatgcttt gcccgtccgg ctgcactttc aaattgaatt
    28321 ccgtctcttt gacggctgcg gtggccacgg gggtttcgac ctcgaaggcc ttcgggccat
    28381 caccggcggt cttggcccac acttcgcctt gactaagccg gaggatccat gtggtcggtt
    28441 tgtccttttg ccagcgggag agaatcttga acgaggtctc ctcgttcagc gccacgttga
    28501 cgccttgttt cagttgaatg taggcctggc tggaagcgtc cgtcttgagg acgtcacctt
    28561 catagagcgg cagggtcttc cggccttgga gctgttcctc tacgccgtcg gcgcggatga
    28621 tggtcaaggt gccgatagca ttgaccgccc atcctaccgg agcattggcg gcccatgcgg
    28681 gtgccactcc accagcaagt aatcccaaca tgatgaccgt agatctgagt aatgatgacg
    28741 tcataaattc ctcttcgcgt cttgtcgccg cgcgttatgt cagttcagtg tgtcgaacgc
    28801 ccgcatcccc aagatgcgcg cggtacatgc gtccggccgc tatctcgaga ccacgactcg
    28861 aatcggcttg cggccgaccg gcaccgtgcc gagattttgt cccgtgcccg tgtcgatgat
    28921 gcgcacttcg ttacccttgt cgtgaccgac gaagatgcgg gtgccctccg ggtttgccca
    28981 gatgccgttc ggcccttcac ccacggcgaa ttcctccagg accttcaggt tcttgggatc
    29041 tgccggcacc atcgccacct tgttggtccc gccgacggtg acaaacacat taggcccctg
    29101 catgcctttg aacccgaacg cgatctgctg cggcttcgca ccgacctcca atgtgccgga
    29161 gatttcctgc gtggtcggat cgatcacgta gatgtccgtt ccaccggtat tggtccccca
    29221 gatgtacccc ttggggccga ccacgaggaa gtgaggattg gcgcctacct gcagagattt
    29281 cacgatttgg tgcgtggcgg tatcgatgat gtcgacggtc ttgtcgccct gggtgctgac
    29341 gtaggcgaat ttgccgtcgt gcgaaaaagt caggccggtc ggtcccttgc cggtggggat
    29401 ggtgcccaca atcctgaagg tctccgtatc cacgatcgaa atattgttgt cgccgatgtt
    29461 ggcgacccac gcctgtttat tgtcgttggt gagtgccacc acatgacagc gttgcccggt
    29521 ataaatggat gcgatggttt ccatcgtgcc tgcgtccatt actgagagcc ggccgctggc
    29581 gaggttcgtg gcatagacgc gccggccatc gcgcgagacg gcgatgtcat gcgtggattg
    29641 gttttttgaa tcgatgttgc cgaccggcgc aaagctcgtc gcgtcaatga ccgagacgtc
    29701 gttcgagctt tcattggcgg tataaatgcg cgatggaata tcgatcggcg ccttgacggt
    29761 ttcgacttcg gcgcaaccgg ccagaccgat gagcccaagc gtggccgcgc cgatgcgtgc
    29821 ccatgacgat gacataacaa tcctccttac tgatctatga acatgacaga atgataaagt
    29881 gccctatata agcgacgatg tgctaccgca cctgtcccag gatcaattgc ccttccactt
    29941 cacccttctt cacgggatgc tgattctgcc ccgtgacctc cggcaccttc ttggagacgt
    30001 agccgtaggc ttcatcgacg gtaatgacgc cgtcgcgatc ggtgtccgcg ttgccgcgca
    30061 ggccttcgag caaatagtac gtgaacacgc cgtgtcccag gtcttcgcgc tcctcgctga
    30121 cttcgctggc cttgctcgca ctgaggacga tacgcccttt cccctttgaa agccggctca
    30181 ggaatgtgtc ggagaccacg gcgcggcgcg aggcggtggc gaaggtgcgg cccgccgcgg
    30241 cgccgctgta gcaggaatcg ctgataaaaa tcacgcgctc gggcgcaagc cggttgaaaa
    30301 tcgtctcgac ttcccgcatc ggcaggccgg tcgagtagag gtccttcggg tccgcatcgt
    30361 agggcacgat gtatttttcg aggccgtcgt cgtccgaggc agacgcatcc gcttccggcg
    30421 cgccatgacc ggcgtaataa atgatgaccg tgtccttctc cgccgcttta cgcttcagtt
    30481 ccgttccgag tgtgcgtttc aaattcatca acgtcgcctg ttcgttcaac agtagcgtgg
    30541 cgttctccgg cggaacgccg acattgcgca tcaaatagtc atggaaggcc tgcgcatcca
    30601 catccgcata ccggagcgga tggaccgtct tatatttcga gatgccgatc accacggccc
    30661 agaccttggc acggtccacc tgccggataa cggtgatgac cttctgtgcc gaaagattgt
    30721 cgcggtcgat ggcggacacg acgatttcgt ttttgccctc cttcagcgtc aggcgttccg
    30781 aaaattcgag gttcggttgt gccgccggcg catccggcgc gacggcaatc cctctcgcct
    30841 cacgctgggt caggaggtgg ccgttgacgc gaatctccac ccgcgcgatg cctcgttcac
    30901 tggccgcggc cccggcaagc agaatccggt ccaccgttac gtctgctcca tccgaggggg
    30961 aggcgagtgc gatgaccgga ggggcgttct ggaacaattt cacgaccggc gcaccggcaa
    31021 tcgaaggaat cggcatgtcg cctttcacac gctcgacagg ttcacgcgcg tcgcgtttgg
    31081 cgatccatcc ggtttctttg ggggagatct gcacgcgcca ccagtcgccg agttcgccgg
    31141 tcacggccag gccttgatcc ttggcggcgg acgcgatgac cgaagtttct tcgccggcgc
    31201 cgctgtgaat tttggccgag gcgtcatgca ccgagacgag tttgttggtc gcgatgattt
    31261 gcggcgcggg ccggtgatcg atggcgagct tcagtgtgcc atccagcgtg acgttcatgc
    31321 cgcgctcgcg aatgaagagt cgcaggggga gttcatcggc cttcgtgtcg cgcgcgacaa
    31381 acagattcac gcgggcaatt ttggtttcct ctggcttcaa aggcccgaag tcgagtgtgc
    31441 tctggctcag gcggaagtca cgtgactggg gcaccgtgag atcgatcgtt gtgtgctgag
    31501 ccaggacggg gccgacgttc ttgatcgtga ccagtatgtc gatggcctcg ccggtctgga
    31561 tgcggccatc gctgttcccg accgattggc ctgatccgtc atccagcaat tggtgggtga
    31621 aggcaaaccg tggtcggggt tgtcccgtga gggccagcgc tgcctgcagc ggggcaggcg
    31681 tgaagccgtt gtattcttcg aattcaagcg agaggggaat ggtgccgtcc ggccggtcgg
    31741 gcggaagggg cagcgtgacg gcatcggcca cggactgtcc tggatcgacc ttccccatat
    31801 aaaacagcag gccgttcaat gccggatcgt cgcttttgct tctggcctgg aagcggtaca
    31861 gagggccttt gcccctgttg gtgacggtca cggtcagggt gaccggttgg cccgctttta
    31921 attccatcgg ctgaatggag aggtctgctt ccggcatggg tggtggagag ggggcggcgc
    31981 cggtttcgtt gccgcgttgt gcgatcttct ccgccgtatt tgccgtgacg gttgaaggag
    32041 tgctgggctg cagtcgcacg gttttatctc gcccgccact cgccaccacg ttgccgtcgg
    32101 gagacagggc caccgtcctc gccggtcctt tgtggcttgt gagggcgtag cgctcgcggc
    32161 ctgagaccac atcccacacc cggacgctac catcggctcc gacggaggcg agcgtgcggc
    32221 catcggccgc gaaggagatg ccgaagacct cgcccttatg gccgttcagg gtcaagcgtt
    32281 cctggccgct gtcgacatcc cagagacgga tcgtgccgtc ggcggaagcg ctggcgagga
    32341 ggtggctgac cggagcgaag gccacggcgt gcacggcgcc agtatggccg gtgagcaccg
    32401 ttcgctgccg gctggtggcc agatcccaga ggtggatctg catatcgcta ctgcccgtcg
    32461 ccaccatggt gccgtccggg gagaaggcaa tgctgaagac aatcccgaac tgcccccgca
    32521 ccgatcgaag ttcttttccg tttgcactgt ctaaaaaccg gaggctgccg tcattgccgc
    32581 ctgcggccag catcttgccg tcggcggaaa aggcaacggc gcggactgcg ccgaaattcg
    32641 aggtcacggc gtgcagttca cggcctgatg cgaggtccca catgcgcact gaggtatctg
    32701 cgcttcccga ggccagaaat tttccgttcg gagagaaggc cagggcatgg acggcgccca
    32761 tgtgaccgcg cagcagcttc agatcctgtc cactctcggg atcccagaga cgaatggttt
    32821 gatcggtccc gccggtggca aggacggttc cattgggtga aaacgccagg gtcaggatct
    32881 cactggcgtg accgctgagc aggcgtggcg ctgtggtgga ggggctggcc catgtggagg
    32941 gtaccaggag cgtgaagagg cacaggagcg ccgtgacaac gaacaggttc acgcgcatgg
    33001 gggctatcct tttccgacgg aggtgccggc ctgtctcacc gttcggatca gatgtctcac
    33061 aaaaaccagt cttccgcttg tcctgttcct gtctgtcggg tttccctgcc tggaggttca
    33121 cccgttttgc ggcggcacca gtgtattttt cggcattccc gattgtcaat ccacaggcgc
    33181 gggcagccgg tcaaatacag gtattggcag gtatacgctt gctgtacggt cttgattcag
    33241 tgctcaagag tggagactcg tagagggcac gtaagttcaa tgtggaggag ggcgacaatc
    33301 agttggccgg atcagtcggc agcttggcga tcttgatcaa ggtgtgttca tcattgagtt
    33361 cgaaggtgac atggtccccg acgctgaggc catgcagaag gcgggcatcc cgaacggaaa
    33421 gtgcgcgaga ctctccggtg gggaaaagaa tggtcaggct gagcgcttcc tgattgatcg
    33481 agacaatcgt gccgcccagc agagcggcct ctgcagatgt ggctgggagg acgacatgca
    33541 ccggcagggg ataggggagg attagaaaag cgagggtgag gatgagcgaa agcatccgac
    33601 acgatagcgc agctcgtgcc tgtgtggaac tagcgtttcc cctatagcga cagtgcgcag
    33661 aagacctaca gcaagagccg gggattttcc ggttgcgctg atcgtatggt cgatctatgg
    33721 tgggaggaca tgccgccatc caaatcagat gtcccagccc tgctgaccgt caacgccggg
    33781 tcgtccagca tcaaatgggc gttgttccac ctgggagcgt cgcctgtccg gacggcctct
    33841 gggcgaatcg agcgcatcgg gatacctgac ggcatcgtga cggtgactga tagagcaacc
    33901 ggccaggatc agcgccatat cgcacagatt ccgaaccacg cggctgccgt tcagatcctc
    33961 atcgatcaac tcgggcagag cggccgggga tcgtcgctac aggccatcgg ccatcgagtc
    34021 gtgcatggag gaagccgtta tacgaatccg gaagtgatct cgacggcggt catggaagaa
    34081 ttgcgtcggc tcagccccta cgatcctgaa catcttccgg cggaactcac catcatcgac
    34141 gcgctgcgcg ctcaatatcc gaccgtccct caagtggctt gtttcgatac gggctttcat
    34201 cagcatttgc cggcggtcgc gcggctgctg gcgattcctc gacggtacga aaaacagggc
    34261 gtccgtcgat acggctttca cgggttgtcc tatgcctacc tcatggagga attggagcgg
    34321 gtggccggcc atgaggccgc gcagggcaaa gtggtcctgg ctcatttggg aaacggagcc
    34381 agcctcgccg ccgtgcgtga tggcgtgagc atcgacacga cgatgggatt cactccggcc
    34441 tcaggggtgc cgatgagcac caggtccggc gatctggacc cgggtctggt gtcctacctc
    34501 tctcagaccg aggggatgac ggcggaggag ttccatcaca tggtacacgc gcagtcgggg
    34561 ctgttgggat tgtctgagct cagtcctgac ctacgggatt tgctggcgca ggaacgccac
    34621 gatacgcgcg ccgctgaggc cgtcgcggtc ttttgttatc aggggaaaaa gtggatcggt
    34681 gcttatgcgg cggcgctcgg tggattggac cagctcgtct tcagcggcgg gatcggcgaa
    34741 cattcatccg tcgtgcgggc gcgcatgtgt gacggcctgg catttctcgg cattcagttg
    34801 gaccaggcca ggaacgacgc ccatgaagcc gtcatctctg cgcctggtag ccgggtcacc
    34861 gtgcgggtga tccatacgga tgaagaacgc cacattgcac aatcggtctg ccggctgttg
    34921 gacttgcccc tcagtggcgg gacgaaatga accgggcgtc agacggatag gcacgaggcg
    34981 accgggtatg ctcacatctg aaagaggaac ttcatgacac agacgacgca cacgaatcag
    35041 ccgctgagcg ccgaaatggt tcaacggatc catgcctact ggcgcgcggc gaactatctc
    35101 tccgtcgggc agatttactt atacgacaac ccgctgctca agcagccgtt gaagcgggaa
    35161 catatcaagc cgcgcctgct cggccattgg gggaccacgc cggggctgaa tttcatttat
    35221 gcccatctta atcgcatcat caaggcgcag aatcttccgg tgctctacat tgccgggcca
    35281 ggtcatggtg gtccggggct ggtcgccaat gtctatcttg agggcaccta cagcgaggtc
    35341 tatcccaaca tctcgcaaga cgaggcgggg ctccagcggc tgttcaaaca gttttcattc
    35401 cccggcggca ttccgagcca tgtcgcaccg gaaacgcccg gctccattca cgagggtggc
    35461 gagttgggat attccctggc ccatgcctat ggcgcggcct tcgatcatcc tgaactgctg
    35521 gtggcatgcg tcatcggcga cggggaagca gaaaccgggc cgctggcggg cagttggcat
    35581 gccaataagt ttctgaaccc cgtgcgggac ggagccgtcc tgccgattct gcatctgaac
    35641 ggctacaaaa tcgccggccc caccgtgctc gcccgcatgc cgccggatga actcgaatcc
    35701 ctgctgatcg gctatggcca tcagccgttt tttgtcgagg gtgatgatcc ggagacaatg
    35761 catcaattga tggcctcaac gctcgatgaa atcgtggcga ctatccggcg cattcagcag
    35821 gaggcacgat cccatggttt tacccgtcgg ccgcgctggc ccatgattgt gttacgcacc
    35881 ccgaagggat ggaccggccc caaagaggtt gatggcaagt tgacggaagg gaccttccgt
    35941 tcgcaccagg tgccgatggc cgagatggat aagccggggc atgtcgagtt gttggagcgc
    36001 tggctgaaga gttacaaacc cgaagaactg ttcgacgaca caggaaagct gatgccggag
    36061 ctggcttcgc tggcgccgac cggtgagcga cgcatgggcg ccatcgcctg tgcaaatggc
    36121 ggcatcatcc tgcgcgatct gcacatgccc gactttcggc aatacgccgt gcccgtggtc
    36181 aagcctggtg cggaggaggc cgaagccacc agggtgcagg gactgtttct tcgcgacgtg
    36241 ttgaaactga acatgcgcaa cttccggatc ttcgggccgg acgagacggc ctcgaaccgc
    36301 tggggcgccg tgttcgaagt gaccgaccgg tgctcgacgg aagagattct ccccactgac
    36361 gaacatgtgt cccacgatgg acgtgtgatg gagattttga gcgagcacct ctgccagggc
    36421 tggctcgagg gctatctgtt gaccggccgc cacggcttta tgaattgcta cgaagcgttc
    36481 attcacatcg ttgattccat gttcaaccag catgccaaat ggttgaagac ctcgcgcgag
    36541 attccctggc ggcggccgat cgcctcgctc aactatctgt tgacgtccca tgtctggcgg
    36601 caggaccaca atgggttcag ccaccaggat ccgggattta tcgaccatgt cgtgaataag
    36661 aaggcggagg tgattcgtgt ctacctgccg ccggatgcca acactctgct gtcggtcacc
    36721 gatcactgcc tgcgcagccg tgactatgtg aacgttgtcg tggcgggaaa acagccggcg
    36781 ccgcaatggt tggacatgga gtccgccatc ctccattgca cggccggcat cggcatttgg
    36841 gaatgggcca gcaatgaccg ggggagcgac ccggatgtcg tgatggcctg ttgcggcgat
    36901 gtgccgacca tggagacgct ggcggccgtg gagattctgc gtgtcgccca gcccaacctg
    36961 aaggtgcgtg tggtcaacgt ggtcgatctg atgaagctgc agcccgccag cgaacatccg
    37021 cacggaatgt ccgacaaaga gttcgatgcg ctcttcacca ccgataagcc gatcatcttc
    37081 gctttccatg ggtatccctg gttgatccac cggcttacct accggcggac gaaccatcac
    37141 aacctgcatg tgcgaggcta caaagaagaa ggcacgacga cgacgccgtt cgatatggtg
    37201 gtgttgaatg atctcgaccg gttccatctc gtagccgacg ttgtcgaccg tgtgccgcaa
    37261 ctcggggcgc gggctgccta tctcaagcag gagatgcggg ataagcgcat gcagcacaag
    37321 cagtacatcg agatccatgg caccgacatg cctgagatca ggaactggaa gtggagcgga
    37381 aagcgctaag ttatggcagt acctagggtg gtggcgttgt tcgatgtcga taatacgtta
    37441 atggacaacg accggattac ggccgacctt acgcaacacc tggatgaaga aatcggcgcg
    37501 aggggggagc gccagtattg ggccatcttc gaagaattgc gggaaaagct cggatacgtg
    37561 gattacctgg gcgcgctgca gcgctatcgg caggagtatc cccgcgatcc ccatctgttg
    37621 gccctgtccc ggttcctgct gaattacccc ttcacggagc gcgtgtttcc tcaggtgcat
    37681 gaggtgattc gccagacggc tcagtgggcg aaacccgtga ttctttccga tggcgacgtg
    37741 gtgtttcagc cgcacaaggt cgaccgctcc ggtctctttg acgcggtcca gggccatgtc
    37801 ctcatctaca tccacaagga gcaggagctg gacgacgtcg agcagcggta tccggccgac
    37861 cactatgtct tgattgacga caagttgcgc attcttacgg cggtgaaagc ccgttggggc
    37921 gcgcgcgtga cgaccgtgtt cccgcggcag ggacattatg cgaaggaccc ggaggtgctg
    37981 cgaacatacc cggcggccga cctcagcatc gatcgcatcg gcgacttgct cacgctcgat
    38041 cggagcgcgc ttctggccgc cgcgaagcca cgtacatgac gagcagtcgg aaggttgtgt
    38101 gcctatcatc cacctctagc aggcaggtga ttcatgacac ccaagacacc cgtgaacaga
    38161 atctggtccc tgatgctcgg ccgccgtcaa cttttgaaag gactgggcgt ggccggctcc
    38221 ttcatggcgt taggaggtcc cggccggttg gccgaagcac tcgccgcaga ctctcctgcg
    38281 accggcacct cctccggaga cattccacgt cgggcgttgg gcaaaaccgg cgtgcaggtc
    38341 tcggccctct gtttcggcgg cgcccattgg ggccgcattc aggatgatgg cgaggcgatt
    38401 cgcttgttac acgcggccat cgatgcgggc gtaaattttt tcgacaatgc ctgggagtac
    38461 aacggcggac ggtccgaaga gctgatgggc aaggcgctgc agggaaaacg gcagcaggta
    38521 gtgctgatga cgaaagtctg ctcgcacggg cgtgacaaga aggtggcgct tcagcagctg
    38581 gatgagtcgc tccgccggtt gaagaccgat tatctcgatc tctggcagat ccatgaagtg
    38641 gtctatgaag atgatcccga tcggcatttc gcgcccaacg gcgcgacgga agccttgctg
    38701 gaggcgaagc gacaggggaa ggtgcggttt atcggattca cggggcacaa acatcctgat
    38761 atccacctga agatgctgtc gcatgacttt ccattcgata cctgccagat gccgttgaac
    38821 gtcttcgacg ggacctatcg cagctttgag cgggaagtgc tcccggtgct gaaccagcgg
    38881 ggcattgcgc cgttgggcat gaagagtttg accggtaatg ccgagcccat caaacaaggc
    38941 atcgttcagc ctgaggaggc catccggtac gtcttgagct tgccgattgc gtcgttagtg
    39001 agcgggatcg actcgcccca ggttctgaaa cagaacctcg atatcgcgcg gcggtttacg
    39061 cccatgacca tcgccgaaat ggagggactg cgcgccagag tcgcgatgtt tgcgatggac
    39121 gggcggtacg aactgttcaa gtccacgaat cgctacgacg gtcccattgg ccgccagcag
    39181 cacggaatct cttgaccggt tcagcgtggt gcctaggcac aggtccggca ctcgcctgtg
    39241 aagacgcgag cgccggccta tgttgggtta gatgtattcg atcacgcagt gaccgtttcc
    39301 tcggtcactc gttggactcg atcctctaac gcacttgcca gaaacccact gtccgccaat
    39361 cgaacagcca ttctcgcctc cctgcgcaga tccttgctca aagccatcaa gccctcttct
    39421 gttcctgtgc ggatggcttt atccaggact tcatctgcag ccggcttccc cgggaacgtc
    39481 aatttgggat tgtgtgggcc gacctcatgc aggagttgtc tgagaatgcc ccctgcgacg
    39541 tatcgctcga atggtaggtc gatgagttga aagcgatcga agagcatggg ccattcgtgg
    39601 cgcggaattt tctctttgaa gatcggttct ttaaagataa attcgctgac tgtgcaacgt
    39661 gatgctcggc cgccgagcgc atcgatggct tcttctccga aacgcgtcag gcttgccgca
    39721 ggagacagtt caaccatacg gtctgtccag agaattgacc accaagcggc atttctcatt
    39781 tcaatttgtc gtgccggagg agcgagccag actccgcaat tcagaaagta cgcgtcgatc
    39841 ttttttaacg ccagctggcc ggctggtgtt tcatcgaacc cggagtatgg cgacggagga
    39901 gcaaatcggc ccgtcaggtt gatgtcgaac caatggtgcc aggtggaatc ggcaagaatc
    39961 cggccgacat ctacattgtg accgttatag gccgatatca ctccgatttc ttgcccatgt
    40021 ttggtggcgt tcgggtcttt aattcttccc catgcaatga cttcaggagt ctcttgatgg
    40081 ccgcctttgg ttggccatgc ggggtcgcct gatactggtg tgggggcgag agcttcacct
    40141 tcgtgctgat ggtctggcag gaaattgatc ggcccgtcgg gtccgcataa cacggggtgg
    40201 ggacgatagc gcctagtcaa aagcaagggg gaggcgatgg tgtagcgctt ccaacgtatt
    40261 gattgtggtg tatcatcgga ttggtcgtcg aaatcgaatg tagatcctgc atcaggtcca
    40321 tcttcaagtg tggtgttcca cgtcggtccg taatccggcg gcgcaggata ccgtctcatg
    40381 acgcccgcgc gcgggatttg tccggagatt ccccttccca ggttgtcatg atcccccgtg
    40441 accagaaccc ctccgaactt cggccccgcc atgaactgtg tcaggagtgc gacctcagcc
    40501 gcagtgagat ttggggtcga ctcaataccg aagaaccaga tctcatcata cttgttcata
    40561 atgtcgagtg cgggatcggt caactgtctg gcgccggcaa ttgtcgccga agggtcgcct
    40621 ccggttcggt gagcaaagtc gatggcgaag tcgacataag agtacgcacg agtctttaat
    40681 agattgtaga cgtactgaag gccaaggaat gatccgccgt tgaagtgtac gctgccgtcc
    40741 gcgtacatga gaatcctgac cttagcggga cgacgccacc aggggaaatg aagatcccaa
    40801 tcgatcgctc cccagcgtcg acgaatcgac tgttgcagaa tttgttcatc cgagaatgaa
    40861 tgtgccataa gacctccttg tgtagtcaaa agctcgtcat gagccctggt taaagatgag
    40921 actcgttcga gtcaccacgc tgattgagcg ctgcatcatt gaacgagaat gcgattcact
    40981 agctagcaag acctgctcca caattgatgg gaagaaaaac ccattacaca atcacagagt
    41041 tgaaatacgg ggtttgagaa atgtgaattt ctgccagtga atactggttt attgattcag
    41101 atacattgag tatcgtggcg gatacgcatt ggcaatgatg gcatgctgtg gcgacggatc
    41161 aacggaagat cggcaaattc gaacgggcta tggcgtggga gaaagggggg aatttgatga
    41221 aacgactaga ccaccatttt aggaacatcc ctgaacagcg gttagccgct catcgccatt
    41281 cgacggcgcc gaagccggaa tccccgagga tgacatacat cagactgacg gccgtgaccg
    41341 tctgattgcg gaattcgacg cttggatagt gcgcatcccg gtgggagaga gaataccgaa
    41401 gcgccacgcc gtgccggtcg aacacccgaa gcgtcaatga agattctgcc cggacgatat
    41461 tctcctgccc atgccgttct ggagagagaa agtcgctgac gtaatattcc cggcccgtaa
    41521 tgtctagcat ggcgcggttc gcataaatca ggcgaagcgc cagcagcgcc tgcggtgtcg
    41581 tcccataatg gtaatcccgt tcttccgtgc gctgaatgct cccggccgct ccatatcctg
    41641 cgccacccaa gaatgttcct tgaagtgcca gttcgtcggc taaccaggac tgccacacgg
    41701 ttcccagtga gagagcggtg ctggagaccc ggaagacttg cggcgagata tagtcatagc
    41761 tcccgaacaa accccagatt ccgcgggttg tgtcgccgga tgcgtaggtc ttccccgcga
    41821 gcagcccccg tgaggtgatg gtttccaggg tattggccgt cgcggcggag acatgaaaat
    41881 caaaatagtc gaacgggcgt gcgtagctgt agcctggctt gccgggtaga ccgtacgtca
    41941 gagtgaattc tcccaccgcg ccgtgctcgt tcacgttcga cgccacattg tggctgctcg
    42001 aggtcagcgt gcctccacca gcgagacgaa tgaacgtcgc gggccggtta ctggggtaga
    42061 ccgcatcgaa gcggttaccg aataccagcc gattgaatcc ggtgggcggt gaaatgatgg
    42121 cggcgcccag ttcgcgccaa aagcccgggc ggccatcgtc tgattcgagc aggagattgg
    42181 ccattctgaa taatggctcg cccaggaatg ttccgccgat gggggtcgcg atcatgtcat
    42241 tgatcgaagg attcgttttt tcgccgccga tttcccagag gaaactgccg gcgacgctat
    42301 acagcgacga ctcccaaaag ttgagtccgg atgagcgggc cagcccaaag tagatgctgc
    42361 cgctgtaggg gtgcaggaat tggttgacgg aaaactggtc gttgtcgagc acccatttgg
    42421 aatcggtcag gtgttgacga atggtactgc cggtcgtgcg gtaatctgac atcggttcgg
    42481 tgaagtgtcg atcaaattgg ttcaacagga agagatacgc gagaatttcc gcagcgggga
    42541 tcagataact gcggccctct ctcgtctccc aattgagtgc cgaggaccgg cctgacggat
    42601 tcgacggact ttctgtccga tcagcgtgat tgactggccc gaccagattc cgttggccat
    42661 tgtccgcggc gggtgtgagc ggctgatcct gagacaggct catgcccggg gacattgaag
    42721 ccagcgccga aaccagcgtg atcattcgga taaggtgatt gttgcgcagg acggacagca
    42781 tgctgtgtca gggctcccac cgtgagttga tcccatcagt ccgcttcggg acactcgggg
    42841 aaaccctagc tcctgacgca gaatgcctag ttgcgcagag agatgccgtg tcagcggggt
    42901 tttatcagcc gctgaaggct gctgccggca gcaaggcggg cgaactgtac cttcaagccg
    42961 ttgacccggg cgaggtaact gctggcgtga tgatcaccgc atttctggtg gggcgcgagg
    43021 tggaacccgc gcgcaacgaa cgcgtgcccg gctcccgctc cgcagaggtg gatgacagcc
    43081 gcaaggtcct gcttctgttg cctgctgata gaggcgcttc gccgggaacc gatcgccgcg
    43141 gcgacctgac gatcgagcaa ggcggcggtc agttcgatgg catggctcgg aacaatgcgt
    43201 gtgtagaagc tgttgaacca acaagcatgc atatcattcc aggggccgtc ttcaaccacc
    43261 acatggttgt ggatgcagta gcgtttcgct tctcggaatg tgccatcggt caactggaac
    43321 atcccgacgg cgctagaagc cggtcggtac caatcgaacg gattccagga caatcgccag
    43381 cgccaatagg ttcgtgccac gggattgccc gatccttcca cttgtgccag cgcggcgaga
    43441 aggtccgggg tgatgatggc cgtcgaatgc tcacgaaaga gcggcccgta ttcctgccag
    43501 gtggccgcgg gattcttatc cagcgcatcc tctagaggaa acaacacttc ggtcggctta
    43561 ttgatcgcgt gataggtcca gttcagggtt ccccagagac aggcgatgag gatgatgccg
    43621 ataagcagac gcgggagggg atgcgtattg ccccacgcgc gaaggaacgc tcgaaggtca
    43681 cgccagcggc gtccggtgaa ccgccagagc gaggtgaatg tgaggcggcg acgggagcgg
    43741 cgtctgtggg gggaatatgt gccggcacga tgagtcactc gaggcgccat cgcctcacta
    43801 tacccgaaat gggctcatga gctactcagt tattggctac ccgtatgtgg gatcgctccc
    43861 cggaagcgag gcctcgcaga gagctcttgc tgatgcgcgc cgttatcgcc cgtggtggcc
    43921 gccgctgctg tgggaatggc cgcctccgct atgcccggca taaccgccgg aagatcgggg
    43981 cgccatgctg accgatggac tgtgttgcgg cggtactgag gaaaggctct gaacattcgg
    44041 cggggtgggg cgccagaaat gattcgggtg gacctggtaa ggcggcgcgt gcggcgtgta
    44101 ttgcggagtc gagggaatcg atcgtggtgt cgggatgacc accacgtcac ctaccttggc
    44161 ctgctggccc tggctgttga ggagtggcgc ggcctgctgg tgatgcacgt gaggcacagg
    44221 gggaactaac agcgaggcgc gcggggccgt ggagaccggc ggtgatgcgg gtggagcgct
    44281 gaggttcgcc atgatggttc tctgcgccag cgggaggtgg cgatgatgcg gcgaaagtcg
    44341 ctgcgcttgc agcaacaggc cgttgatgcc tgatacgccg atccatgggt atgagtagaa
    44401 gcccaatgtt ggatatgtcc aactcgtcca ggcctgcgac aggagcgcgt tcgcctgcaa
    44461 ctggtcgagc atatcctgac gttgatccgt gtattgctgc acgtcgtaag gcgtagaagc
    44521 ggcggccagc gcgcgattgg tgtcgtcgac ctgtgcctgg agtcgatcgg tttcagcccg
    44581 gagcgcgaga agttcttgcc gggcctggtc gttctccctg agcgcggcaa tggcctgaat
    44641 gacgtcctgc ggctcaactt ctcctgtgat gtccacacga atcacgactt aatcctgttc
    44701 cagcctggtc gtcaccgtct ggtcaatcac cctgacgact ccggcggtga agctacggat
    44761 atcatcacgc gtcacgttca agtctttcac ttccgtgatg cgctcgacgt aggttgccac
    44821 ctgttccagc gcgtcttgct tcgcggcttc cgtcgcgagg cgaacggcat cggcgcggtt
    44881 gtcgtgatca cccatacggt attcacccct ggcggtgatc gtgcgcgctt cggccgaacg
    44941 cggacgaagt tcggatgcca tgtcaggtgc aggcgtttcc ggacgtgccg gtgtgtcgaa
    45001 gctgcgctgg gtcagctgcg ggcggtattt ctccgggacc gatgccaatt cgttggtaaa
    45061 agtctgcacg ccctcgtcgt tcgtgtagct gtaaatggtc gtcgaggcga aagagggagc
    45121 cgtaggaagc acggagagcc agaggacgag gagtgcggcg gtggtcggtg tgctgcggga
    45181 gaagaacatg gtacagcctc ccgttcgtct acagggtcag gataacacaa ggagggcaac
    45241 cgattctgct ggccagggct tagatatgtt gggcgatacg agggcgggtt tgtcgtgtgg
    45301 ggaagtcctt ctacagggag tgtccataga agagcgggat ctcgggattt actggctcct
    45361 cctccagatc gcgctaggat ggaatcatcc tatgctggtc gtcaaacgaa tcatcgctgt
    45421 tgtgtttggg ctcgtcccgc ttgtgctcat tgcggctggg gtcgcaatgg ttttccggga
    45481 actccggccc actgatccgt tgttgtattc cgtgcagcag gatggggaac tccccgaggc
    45541 cttcatgttt gcggctgtgg ggtgcgccgg attattcggc tgccaccgtc tgtggcgagc
    45601 cgatgccggc tggaagtgga ccctcatgcc gattgccgtg atggtggctg cgatagccat
    45661 tcccaatctt ctctatggac accatggatc gccgtcggat cgagcctact ccaatatgtt
    45721 cctgcggctg ggcacgttcg ccgtttcctc aaagcagctg gcccaggaac aaggccagtt
    45781 tacctgtgat ccctccgtga aactattcgg gctatctcgt ttcacccaaa acggtcagcc
    45841 tcttccctat gtgatccact gtgtgctcga tgcgactggt ctggcactgg ccactccgcc
    45901 tgaacggccc ggaacgctgg tcttcgccgt cagtcctgac aggaagcagg cctggtttgc
    45961 cgccacagtt ttagcacgcc ggaccgatcg gcatgcgacc tggttgaggc agcaaggcca
    46021 gccgcgggtt atcgcgttca gtgtgcagga gaagaactaa gggagacggt gcgctcggac
    46081 gttcaatttt gttcgccttc gtcgatcatc taatctaatt tcctggaaat ggtctacgct
    46141 ggaattgaag ccttcaaggc aacttgtgta tcctacggcc cgttgtcgcc accctgctct
    46201 acgaggtgcc agcatgagcc agccttcacc gtctgttcct tccaaccttt tcttgctgtg
    46261 catttcgggc ccggatctcg gcaaacgcat cgcattgcgt gatggagaac tgctgatcgg
    46321 ccgatctctg caatgtgatg tgctgagcga cgatcaggat gtggtggagc ggcatgcggt
    46381 cgtgctgtgg gagaacggcc aactcatctg ccgggcagtc caaggcatcg tgtacctcga
    46441 tggacacaag acgatggagt ccgtcctgac gccgcagcag cagatacgga tggggcggtc
    46501 cttgtggcaa ctcaacgggc cgacgccggg gagaggctcc ggagggtgga tcgaagatct
    46561 gagcgatcgc atcagcgagg tggcaggggt agagcggctt caaggcttca atgcgcgcga
    46621 catgttttcc gacgtgctgc gcaagcggac cgacgaggaa attgaagagc atttcacggc
    46681 gggaacgcta accaccacac cgccgattga aaaggtgata accggctggc cgaagccctg
    46741 ggtctttttt aaaaccttca ttctctcggc ggtcgtctat ctggcgtttg tgttcgccat
    46801 gaaggaattc aataacgcca atctcgtgcc ggggctcatt atgacaggtg cgttcctcat
    46861 tccgtcgtcc ctgctcatct tcttttttga gatgaacgtc cttcggaatg tttcactgta
    46921 tcaggtcgtg aagatggtga tgttcggcgg cgtcacgtcg ctgattttat cgctcttcct
    46981 tttcaagtgg acggacctcg acagttggat gggcgcgatg agcgccgggg tcatcgagga
    47041 gacggggaag gcgttggcgc tcctgctcat catcaacaat caaaaatacc gcacggcgct
    47101 taacggtctg ttgtttggag cggcggtggg cacgggtttc tccgcgttcg agtccgccgg
    47161 ctatgccctc atggccgggc tgagcggcgg acagtcggct atgttgcaca gcatcacggt
    47221 gcggggtctc ttgagtatgc tgggcctgca tattgtatgg acctccatgg tcggggccgc
    47281 actctggcgc atcaggggga atcgatcctt caccattgag atgctgtacg acaagcgatt
    47341 tctccgcatc ttcggattgg cggtggcgtt gcatatgttc tggaacgcgc cgttcaatct
    47401 gccgttttat ctcaagtata ttgcgttggg cttcatcgcc tgggtcgtga atctggccct
    47461 ggtgcaggtt ggcctcagag aagtgaaggc gttgcaagag gcacaggcgt ccgaggcttc
    47521 ggattcgaga ttgagaacgg cgtagggcac atcgcctttg catgagccat ggaggatcag
    47581 cttgaaggag gagaatcgtt ggatcgtgag ccagctgtcg gagacacggc tagctgttcg
    47641 acgaattgtc gtgcttcttc cagtgtcata tctttcgtca tgcgaacgag tttgatcgcg
    47701 tcgagtttgc ggccttgagc caaagcggct gttaggctgt ccagcaagaa attggttcgt
    47761 tctgcgttca tttgcatcag gcgacggatc gtaatcgcca actcttcata ctctcgcctc
    47821 atcccgcctg cgtaggctct cgagagcgga atttccctct cgtctgtcac tagcgccagc
    47881 cgcactaagg gagcggtttt cgtgcttccg atcgcggctt gtactgtaag cgagcgcagg
    47941 catgaaaaag gcatacggcc ttcatccttt gcc
//
LOCUS       DAOQDS010000031        47351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_243_length_47351_cov_24.688874, whole genome shotgun sequence.
ACCESSION   DAOQDS010000031 DAOQDS010000000
VERSION     DAOQDS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 47351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47351
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_243_length_47351_cov_24.688874"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PK589_09405"
     CDS             <1..314
                     /locus_tag="PK589_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNJ19859.1"
                     /translation="SDAALKEVKRLINVQGIEEGGLRLGVKGGGCSGLSYTINFDEKI
                     GQYDQVYDFDGVKVIVDAKSAIYLQGTQLDFQKDLMGGNFKFVNPNANKTCGCGESFS
                     A"
     gene            329..1045
                     /gene="hscB"
                     /locus_tag="PK589_09410"
     CDS             329..1045
                     /gene="hscB"
                     /locus_tag="PK589_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNJ19860.1"
                     /translation="MSGHTHNADDRRELQMARSMCWHCQSEMAGEYFCDRCVKVQPVS
                     KDLDYFTCFGLPRRLAIDTQVLESKFYELSRAFHPDFYQNKSDAEQSISLGNSAMLNT
                     AYRTLRDPIQRAEYLLDLEAGSVKDIRTSPPADLFEEILELQETLDEFRESDRSSEHA
                     STLRAKLHTDRTNLEERQRDMEAKLQQLFSRWDALQDRGEATEQARAERTLILKDMRD
                     ILSNRTYVKNIVNDLVATIA"
     gene            1051..2871
                     /gene="dnaK"
                     /locus_tag="PK589_09415"
     CDS             1051..2871
                     /gene="dnaK"
                     /locus_tag="PK589_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNJ19861.1"
                     /translation="MARIVGIDLGTTNSLVAYMDKGTPRVISARHERAMVPSVVALTD
                     NGLIVGDPAKEHLTRNPERTVYSVKRFMGKSLADVQGELAYFPYTLTEKGGVIRIQLG
                     EKSYSPPQISAMILKELKLRAEAFLGESITKAVITVPAYFNDSQRQATKDAGMIAGLE
                     VLRIINEPTAASLAYGLQKKTQGTIAVYDLGGGTFDISILKLKNGIFEVLATNGDTHL
                     GGDDFDQLIADLLLKDIRAQHGLDLSTHPDHMQAVRLQAERAKIRLSEELKTTVSLDL
                     PDGQGRFSRELTRDQLEALCIPLVERSLGPCRLALKDSGLNPTDIDEVVLVGGSTRMP
                     LVRQRVQALFGKEPHCELNPDEVVALGAAVQADILSGGTTDMLLLDVTPLSLGIETMG
                     GVMSSLIRRNTTIPASAKEMFTTYVDGQTGVDIHILQGERELAKDNRSLARFRLKVPP
                     LPAGVPRIEVTFLIDANGILNVMAKDMRTGETQSIEVKPSYGLSDQEVERMIEDSFKF
                     AADDVSARKVIEARLDAGALITTTEKSLTDGGHLVEAEEIAKIRAALSALAAAKEGTD
                     PRAIRGCMADLEQAAKGLTVAMLDDSLKQGLQGKKISDVT"
     gene            2894..3106
                     /gene="iscX"
                     /locus_tag="PK589_09420"
     CDS             2894..3106
                     /gene="iscX"
                     /locus_tag="PK589_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNJ19862.1"
                     /translation="MDLKWQDAEDIAIRLVEEHPDADPLTIRFTDMHAWIVALPDFKD
                     DPKKSNEKILESIQMAWHEEYQDSRS"
     gene            complement(3222..5618)
                     /locus_tag="PK589_09425"
     CDS             complement(3222..5618)
                     /locus_tag="PK589_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNJ19863.1"
                     /translation="MSELDHLLDKPEKPRRQRRWWKIILIGLLLAIVLGGGGAAGTLW
                     YFSQDLPSLDPLQNYQPSLVTRVYSDDRQVIGQFFIERRFLKPIQEMPKSLTQAVIAT
                     EDTRFFEHPGLDIVGILRAAWTNLRHGGKKVEGASTITQQLARSLFLSAERTFDRKVR
                     ELILAYKMELVLTKEQILEMYLNQIYFGQGAYGVAAAGQTYFGKELSKLTLAESAFLA
                     GLPKSPSHYSPFKAYDRAKKRQEHVLSRMAEAGFISVADRDQAIAEKLNFRRPGSEHL
                     GPYFVEYIRQLLVAKFGEAMVYKGGLEVFTTLNADMQKAAEAAVFNGLREVDKRQGWR
                     GPLRTVDVTTLEVPAPDPSVKLAEGDMMEGVVTKIAKDHVLVQIGGTTGRLSFDDMAW
                     ASKYLKGKDPTKDAVPVKNIKQLLSPGDVIEVGVKKLDKDVVHLRLEQTPVVEGALVA
                     VDPKTGAIRAMVGGYDFARSEYNRAVLARRQPGSSFKPIIYATAMNQGMSPATVVLDA
                     PVVYEQEEEDKTWKPENYGKRFHGIVSLREALIHSHNLATVRLLDKVGIRNVIDFSRT
                     VGVVSPLAADLSLALGSSSVGLMELVSVYGVFANQGVRVEPYAVASVQDSGGRTLEQA
                     AIQPRQVVSRETAYLITNMMEDVIQRGTGIAAKAVIDRPVAGKTGTTNDFTDAWFIGS
                     TPNLATGAWVGFDDRRPLGETESGAHAALPIWIAFMKEALKQLPVVPFEIPEGVMFVK
                     VDPSTALLTDQDEQRGTVELFTKGTEPTKSAGSKIDPTEFYKLDQAQESASPSPVEPQ
                     "
     gene            5842..7722
                     /gene="typA"
                     /locus_tag="PK589_09430"
     CDS             5842..7722
                     /gene="typA"
                     /locus_tag="PK589_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNJ19864.1"
                     /translation="MNSSAPSSTSGLHAPKGRRTDIRNIAIIAHVDHGKTTLVDAVLR
                     QTHVHRKIDDMGERIMDSMDQERERGITIRAKNASVIYKGVKINIVDTPGHADFGGEV
                     ERTLRMVDGVLILVDAKEGPMPQTTFVLRKALALGHKAIVVVNKIDRPDAVIDDVVNR
                     TFDLFVHLGANDEQLDFPIVYASAIKGIATLDLKQPGTDISPLLETILDKIPAPAINR
                     DDPFQLLVLALAQDSYKGKMGIGKIQSGSIARRQNVVTLTKDGGQVPGKISDLAVFSG
                     LERSDVDSAEAGEIVAISGLEEVNIGDTIADPGSPVALPRVTIDEPTVQMTFSVNNSP
                     FAGRDGKYLTSRHLRERLFKELETNVSLRVQETDSADRFLVAGRGELHLGVLIEQMRR
                     EGYELQISQPEVILHRDGETVTEPFEELTIQVPSEYQGPVIEEIGKRRGELRHMKLVN
                     SEGTASEMHIEYHIPTRGIIGLKNILLAKTRGTIIMHHVFSGYAPADEKALLVAPHGS
                     LVAFEAGTSTAYALFMTQERGELFIGASVEVYQGMVVGQNSRDEDLDVNVCKQKQLSN
                     MRAAGTDEALVLTPPREMSLEFAMEYIGQDELVEVTPKYLRLRKRMLNPDDRRKAKKN
                     AK"
     gene            7758..8513
                     /locus_tag="PK589_09435"
     CDS             7758..8513
                     /locus_tag="PK589_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19865.1"
                     /translation="MKIRKKAPKPSPAKPPLYVIGYRGMPPSLGELKVWYDLHYGGPL
                     TWLEPVAEGRASASHGPWRAHVITSLPDTETAQWQQVLSWDHQELGAVSLSSATPSTI
                     ADTVLVTARLARGLTLLTQGTAFDVASHEYLNPSDWNDRPLDVFVTSDHVTVQHGETD
                     DHSSEWFYTLGLTKFGLDELEVIQPRGLPERETIALLRCAADAVLRKGQNQKVGGTMD
                     LHAVAHTIRFIKHRTAAPTGRMMAFRQISTDLL"
     gene            8671..9141
                     /locus_tag="PK589_09440"
     CDS             8671..9141
                     /locus_tag="PK589_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNJ19866.1"
                     /translation="MSDVAAEIKVGDTAPDFTLKDQDQKDVKLSDYRGKSNVVLAFYP
                     LDWSPVCQGENKCLTDDFPKFQGAHAELFGISCDSFFSHKAWADSLDLKHRLLSDFNR
                     EVVKKYGLYFEPLNCGKRATVIVDKNGKVAYVKVQEIKVAREDKDILAALAKLS"
     gene            9219..9551
                     /locus_tag="PK589_09445"
     CDS             9219..9551
                     /locus_tag="PK589_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNJ19867.1"
                     /translation="MTGTVQDVRDENYKEFTESAGAVVAYGLATCEPCKQYDPILEET
                     AAKFPTIKIGKAKMHVPGRCREIKKAHTFETYPTTHFFAQGKLLLTREGVVEPAELAA
                     LISDHLLK"
     gene            9867..10559
                     /locus_tag="PK589_09450"
     CDS             9867..10559
                     /locus_tag="PK589_09450"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNJ19868.1"
                     /translation="MAFIPAGEFAMGSDRGQDDEQPVHRVLVKAFYLETHEVTVARYA
                     AFLMSQKADPPFKWNEATSGAHESKPVAGVNWYDARDYCRWIGRRLPTEAEWELAARG
                     TEGRMYPWGDAQPTRSHANAGHTKWHGYDTLTNVGQFELGKTPNGVYDLAGNLWEWVA
                     DWYDATYYQFSQRENPSGPSAGPLRALRGGAWNNDAKTIRSANRAGYAPDARRNDVGF
                     RCAKDAPPSNAR"
     gene            complement(10556..11308)
                     /locus_tag="PK589_09455"
     CDS             complement(10556..11308)
                     /locus_tag="PK589_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ19869.1"
                     /translation="MRANHSPESTEQSSVLTIGVTDLMTSLAVIFILLFSAYVTKVSE
                     TEPQTKVKAPVQAPERNVATSTTEDMKGMLRDHFQRFDLSLDADPTDPNIVRIVVPDA
                     LLNFEFGKGTLSSVADRFLVDAMPTYAALLCGAMRDRIDSLVIEGHTDDRGSDIYNLK
                     LSQERSLNVMVKGLEVIKDSAPWAYRCFHEKTSASGRGRQDLVMDHSRALDRDKSRRV
                     VFKIRLRSGDRPGEMSQAMRQLESPAFSSRLF"
     gene            complement(11310..12560)
                     /locus_tag="PK589_09460"
     CDS             complement(11310..12560)
                     /locus_tag="PK589_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19870.1"
                     /translation="MMSLWEFLSQDVTLLGELHSPMLSWLGSGGLIALFMWHAGRLST
                     AISSVQTCYTRVWPTLTGLAASRKSLQNEWLTVPSLSDVKRVANQSGAQPERIDLDDL
                     HTLDKAMRREPRLEQAWLHFRKTFVVERTAWFIEPKVFATRTAAEFFPRDLLNSRLNL
                     AFYHQFPSLITGGGLLLTFLAILIGLSKLHADGSHIVGIQGLINGLAGKFLTSIVGLL
                     CANLFVLLEKSAMHRLATTQQQFVTMVDELFPRKTMEQMLENFTPGAGPTPAQSPAAV
                     GAVPGDLGDRLAGSLSDRLSPTVAALREAVEVLSRRDPGGRTGSPDRLAEELSRVMQQ
                     TMAAPIQELNQAIQTLARSVEELKQDRGVKEVSDEFEFDSLDEVPTAESEQDIQQEVE
                     PDDSMLGLRWFANWRQGASMKGAA"
     gene            complement(12767..13207)
                     /locus_tag="PK589_09465"
     CDS             complement(12767..13207)
                     /locus_tag="PK589_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ19871.1"
                     /translation="MKVITGVAGNPAEGYQVVGDIVGTTIRQFKPGDSAMDVAIEMVT
                     SHVGGAPVVGEDYEFLGFINEGDVIRAIDKGMDLKQARAGDIMNSAFVGVKEDTSLGC
                     VARMFEMGFQILPVVQDGKVVRSITRHDLLRAQLGLGPSVDEGS"
     gene            13389..13718
                     /locus_tag="PK589_09470"
     CDS             13389..13718
                     /locus_tag="PK589_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19872.1"
                     /translation="MTIPWTTALQVARKLLPVVIEKAPELIRTFERFRGAPPAAEPSA
                     SDSALSALQLQIDAQQRTIEQQADTIVELRATVSRIQRALTLARTFLAATISLSLAII
                     AYLLLRP"
     gene            complement(13836..14588)
                     /locus_tag="PK589_09475"
     CDS             complement(13836..14588)
                     /locus_tag="PK589_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNJ19873.1"
                     /translation="MASVLLNKRIEFCASHRYHKPEWDAEKNRATFGACNNDPGHGHN
                     YMLEVTVAGEVDQRTGMVVNLFDLKRVLLQVLEEFDHKHLNLDLPYFNHQIPTSENLA
                     RVLWDKLQAQQDIGTLQRLSLYEDEDLCADLTAEAGLDVASVTRRYSFTAVHEGHRGH
                     TWDLFVSVHGPIHPETGMVTDIVALDRLVKERVLVPFEGRDLRVVLGTPSVTGEYLAK
                     AVWDRIASAVPAGSLQLVKLVQTRDLFYEYAG"
     gene            complement(14677..15261)
                     /locus_tag="PK589_09480"
     CDS             complement(14677..15261)
                     /locus_tag="PK589_09480"
                     /inference="COORDINATES: protein motif:HMM:NF023432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HNJ19874.1"
                     /translation="MNQLTQADLLPPDEYERQREAYRQTIIDLKRRRRISIGDKITMV
                     FENRETLRFQIQEMIRVERILDPQKVQDEVDVYNALLPAPGELSATLLIELTDAETMK
                     HWLDLFMGLDHGQKLGLRAGGEIVYGEFEGGHSHETKISAVHFVRFRPTPAMVTALAD
                     PAVRAALSVRHAGYEADAEVPWTMRQEWLADLRG"
     gene            complement(15288..16574)
                     /locus_tag="PK589_09485"
     CDS             complement(15288..16574)
                     /locus_tag="PK589_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNJ19875.1"
                     /translation="MKELRLLQPIDPATLEQETLRIYDVCDGCRRCFNLCPSFNTLLD
                     RIDDYESDVTKFTPADHHRVVDECYYCKLCYNHCPYTPPHQYAIDFPLLMAVWKKRLA
                     AERGIRWRDWLLTRTDLLGRLNSALAPLVNWGLGQKWVRSLMQMVLGVHQERQVLHYQ
                     QETFARWWGRRPSRNAGATGKKVALFASCLVNYQATDVGKATVQVLEKNGIHVVLPDQ
                     SCCGMPSFDIGDTTAMVKAAEHTIASLKPWVDQGYDVVIPTPSCSLMVKREYANLVSG
                     EDATRVAGRTYDVCEYLMKLKREGSLAMDFIQKPGRVAYQVPCHLRDQNIGFKSKELM
                     ECAGAQVEVIEKCSGHDGSWSAKTEFFPLSMKIAQKAVRQVEQVPADVVASDCPLAGL
                     QLDQAGAQAHVGGKSAQHPIQVVRDAYGLPRDPRTS"
     gene            complement(16681..17106)
                     /locus_tag="PK589_09490"
     CDS             complement(16681..17106)
                     /locus_tag="PK589_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNJ19876.1"
                     /translation="MGNSLKGTKSHENLKHAFAGESQANRRYLYFARRADIEGYPDVG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPATGVPIGNTEANLKSAIEGETYEYTQMYPGM
                     AKTARDEGFSELAEWFETLAKAERSHANRFTKGLDSLKQ"
     gene            complement(17135..17587)
                     /locus_tag="PK589_09495"
     CDS             complement(17135..17587)
                     /locus_tag="PK589_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNJ19877.1"
                     /translation="MSIDLREIETRFRRHAVRVTRQRAAIYAALVETTSHPSADDLYR
                     MVTRQQPMMSRNTVYYTLGVLRNAGLIREVNVGHDMARYDGNVSMHHHLICLGCGQIL
                     DVMDDELNQLHLSSGQARGFEVQGHRVEFHGYCVSCQQAERNVSPRRS"
     gene            complement(17795..18352)
                     /gene="rfbC"
                     /locus_tag="PK589_09500"
     CDS             complement(17795..18352)
                     /gene="rfbC"
                     /locus_tag="PK589_09500"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNJ19878.1"
                     /translation="MQITATPLSGLFQIEPDVFGDARGKFVEIFRESRYGAAGITKPF
                     VQDNFSWSVRGTLRGLHYQLAKPQGKLVMVVKGAVYDVAVDIRQGSPTFGQSYGVELS
                     EANMRQLYIPPGFAHGFCVLSEEAGFLYKCTDVYSPPDERGIIWNDPKLAIAWPITAP
                     LLSAKDQAYKCLADMTAELPRYEAS"
     gene            complement(18469..18870)
                     /locus_tag="PK589_09505"
     CDS             complement(18469..18870)
                     /locus_tag="PK589_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19879.1"
                     /translation="MQSLNIQRPGMPDLQFVLVVSALCTSGLETLNVPEDLRRKVFDA
                     CWALVSTDPPPTIERERVLDLRSGTELTLDALVATIRQLFMEAGISTLTWDHPPSEPT
                     RPSSPAAEPLIDRLQKLYPDPPPTSDPSTRN"
     gene            18973..19245
                     /locus_tag="PK589_09510"
     CDS             18973..19245
                     /locus_tag="PK589_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNJ19880.1"
                     /translation="MITITLMGQLQTTDGERDLACELPTPVTVRQVIQRQGRGLRHML
                     QLLREKKVLVTINKRIASEDSMVQDGDAVRFVGHDGAGGSGLAPSF"
     gene            complement(19352..19639)
                     /locus_tag="PK589_09515"
     CDS             complement(19352..19639)
                     /locus_tag="PK589_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNJ19881.1"
                     /translation="MAKSMTKSQIADHLAGKAGITKKTAVQFLDDFAALAYREAKNAF
                     VVPGIGKLVLANRKARMGRNPQTGEPIKIPAKRVVKFRVAKMAKDSILGKK"
     gene            19936..20493
                     /locus_tag="PK589_09520"
     CDS             19936..20493
                     /locus_tag="PK589_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19882.1"
                     /translation="MAKELPILPSNAQPAEGTAPEAVIYSRVFCKNENAPPLRLLIEF
                     LKSRGQSPILPPDMDDSMLDEWAWVQVILGYARERKPIQVFCIRDRGTYQDLFEQEQK
                     QFREDLSSHDDLEATLAQEHVTRARFILTTRMIEADVTEEGYDFNGWILEFFQEHCNG
                     IVQIDQQGFFSPKGELIVDLSLPEE"
     gene            20508..21098
                     /locus_tag="PK589_09525"
     CDS             20508..21098
                     /locus_tag="PK589_09525"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNJ19883.1"
                     /translation="MHPPLPLFQQASHWFRRAQASLLEALPCSQGCCDCCIGIFPITQ
                     LDVLELHRGLDLLPSDRKAAIVMRAREQVAALELAFPDLRSTPNLDEWTDSNIDAIVE
                     RFADLPCPALAEDGRCSVYAFRPITCRTMGVPIESDGMVQGACRVQTAVPIIRPSPSL
                     RTDYDLLAKDEAVALSILRQAHREAGDELLLPYGFI"
     gene            21166..21242
                     /locus_tag="PK589_09530"
     tRNA            21166..21242
                     /locus_tag="PK589_09530"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21200..21202,aa:Arg,seq:ccg)
     gene            21289..21364
                     /locus_tag="PK589_09535"
     tRNA            21289..21364
                     /locus_tag="PK589_09535"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21322..21324,aa:Lys,seq:ctt)
     gene            22015..23475
                     /locus_tag="PK589_09540"
     CDS             22015..23475
                     /locus_tag="PK589_09540"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNJ19884.1"
                     /translation="MQVFPNRILFVGFGAVARCTLPILLKHIGVDPKRITILDFDPNE
                     AALAPWIKQGVTFVKDRVTPENLGTLLGKHASRGDLLIDLAWNIDCCEIVQWCHDRGV
                     LYINTSVEIWDPYCGVDDKHPTERTLYWRHMNLRRMMASWGEPGPTAVVEHGANPGLI
                     SHFTKHALLDIARQTLADKKFSGEQAERIAHHAQAQEFNHLAHQLGVRVIHCSERDTQ
                     ITDRPKQVDEFVNTWSVEGFREEGTTTAEMGWGTHEKQLPRFAYEHPDGPKSQICLAR
                     MGMNTFVASWVPPDHNIIGMVVRHGEAFSITEKLTVWEGDKAIYRPTVHYAYCPCDAA
                     IASLHELRGSDYRLQSRTRIMTDEIISGADILGVLVMGHPYQSWWCGSHLDIEEARRL
                     VPHQNATTMQVAISVVAACMWMLENPSRGLCVPDNLPHDYILNMSKPYLGKFISAASD
                     WNPLKHYSNFFRGYNQPQLDRSDPWQFKNFLIMEGD"
     gene            23553..24686
                     /locus_tag="PK589_09545"
     CDS             23553..24686
                     /locus_tag="PK589_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HNJ19885.1"
                     /translation="MDQKTLLDLANLHGTPLVVVDHAVLRENYDQFRKHLPRVQVYYA
                     VKANSDPAIVQTFYDVGASFDVASMAEFLIVHEKIKHLPAQQRQDFIWDRIIYANPIK
                     AIETLTQLDQYKPLITYDNHEEVLKIARYAPHSGLVLRLSVPNTGSMVELSSKFGALP
                     GEAVDLIAFAHNNKLVVEGLSFHVGSQCTNVQNYIQALHLAAGIFSEAKTRGFDLKLL
                     DIGGGFPAHYDDSVPAFQSLAKTINAELDRLFPQPIEILAEPGRFLVASAATSVAQII
                     GKAVRGGKLCYYLDDGVYHTYSGVIFDHCQYHLKSFRKDPTQICAVFGPTCDALDTIS
                     LVEQLPDMDRGELVYAENMGAYCGASSTYFNGFPPAKVVHVNQ"
     gene            complement(25076..25504)
                     /locus_tag="PK589_09550"
     CDS             complement(25076..25504)
                     /locus_tag="PK589_09550"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNJ19886.1"
                     /translation="MESAIVTVLVLCTGNSCRSIMAEALINALRPGRYHAVSAGSHPT
                     GYVHPKSIDTLKRHGIDPGQPKSKSWNEFSHQTFDLVITVCDQAAAEACPLFLGAPTK
                     MHWSTPDPAKATGSEVEIDAAFDQAFLMLKKRVEHLTSTT"
     gene            complement(25504..25974)
                     /locus_tag="PK589_09555"
     CDS             complement(25504..25974)
                     /locus_tag="PK589_09555"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HNJ19887.1"
                     /translation="MKRFHLHMGVEDLDTSIRFYTALFGVPPVKTKSDYAKWMLDDPR
                     INFAISTRAKKGVDHLGIQADGEDELAALRGRIQAGHLPVVNEGKTVCCYALSDKSWI
                     QDPSGLPWETYRTMEDAQLFSGSTDSSEGACCTPLTMAPTNCCEPSEKTAGCCD"
     gene            complement(26391..27500)
                     /gene="arsB"
                     /locus_tag="PK589_09560"
     CDS             complement(26391..27500)
                     /gene="arsB"
                     /locus_tag="PK589_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNJ19888.1"
                     /translation="MKLMDAAGVIAQQTLPTEKRLNVFERYLTVWVAFCMVAGMLLGT
                     SAPGLIQMLRGMEFGQDSHVNFPMAVLIWLMITPMMMNVDFRAVAKVGQRPQGLLVTL
                     FVNWVVKPFSMALFAWIFFRFLFSAWMSPAEADQYIAGAIILAAAPCTAMVFVWSYLT
                     DGDPAYTLVQVSVNDLIMLVLFAPIVGLLVSGASSLTVPLAVLVYSVIGFIVIPLVLG
                     VVIRHWFISQRGKTWFEETLLPSFAPMTIGALLVTLVLIFAFQADNILAKPLHVLLIA
                     IPILLQVYFNASLAYGLMKWLKVPYAVAAPGALIGASNFFELAVATAIALFGPESGAA
                     LATVVGVLVEVPVMLSVCHVCNRTRHWFPSKEATA"
     gene            complement(27544..27864)
                     /locus_tag="PK589_09565"
     CDS             complement(27544..27864)
                     /locus_tag="PK589_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNJ19889.1"
                     /translation="MRTKTKQLTDVATVFHALSDETRLLILEQLKGGEQCVCDLTEIF
                     KTGQSRLSFHLRVLKEAGLILARPEGRWVYYSLNSDAVEELQDVVNSLKAVSSRAARQ
                     GCCP"
     gene            complement(28404..28724)
                     /locus_tag="PK589_09570"
     CDS             complement(28404..28724)
                     /locus_tag="PK589_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19890.1"
                     /translation="MNSQQNNKPSRPGRKGALAKLSEPQVGDAPPKLKSKPSKRPMLH
                     DASTVPPQKHVLTQEEFRARVSRKAFELYEIRRSVTEVDDWIAAERLVKVELLSEERG
                     AGSV"
     gene            complement(28765..29730)
                     /gene="corA"
                     /locus_tag="PK589_09575"
     CDS             complement(28765..29730)
                     /gene="corA"
                     /locus_tag="PK589_09575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNJ19891.1"
                     /translation="MLSAFVLEEGRVIQAQIDDKDDLTRKDLVWVDLAYPSEEERELV
                     QSVFPLELPDDEELQDLEASARYYQDQHGIHIRSTFVQTADDVASIVNMSFSLHQGRL
                     LTVHDDDLSLLRLFRMQARSSRGMVRDAADILLSCYELGVERDADVLERIYTALDEVG
                     TVVLSRKKEITSRIMRDNVERIAAQEDLNSKVRLDLIDNRRALSFLLRSRVLSPDQAQ
                     RVKGVLRDIDSLNGHTGFIFDKINFLMDALLGMINLEQNKIIKIFSIAAVVFLPPTLV
                     ASIYGMNFEHMPEMKWPLGYPMALLLMVFAGGAPYLYFRQKGWLD"
     gene            complement(29760..31277)
                     /locus_tag="PK589_09580"
     CDS             complement(29760..31277)
                     /locus_tag="PK589_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNJ19892.1"
                     /translation="MILLFLGTLYTVEQLSFELMAVKQSSAWPFILLGVALVIALGFE
                     FVNGFHDTANAVATVIYTHSLPPHAAVIWSGLWNFIGVMMASGAVAFGIVSLLPVELI
                     LQVSAGAGFAMVFALLIAAIIWNLGTWYIGLPSSSSHTLIGSVIGVGLMNQLMAGSAE
                     ATSGVDWAQALNIGKSLLVSLLFGFSVAAFLLLVAMRAIPIASLYRAPQGKTPPPLWI
                     RGILILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTVYALNRTVDISYTPAFVETSR
                     QAERTLDRYTHGQPAPTDPRHEVGQYLRSRQLMPETIPALRALSAEVREGISAFGSVA
                     QVPDEQTGNLRNDMHLITEALRRMEKQHDPPLSAPDLKILKEYRSTLDTATKFIPTWV
                     KAAVALALGLGTMIGWRRIVVTVGEKIGKAHLTYGQGACAEMVAMGTIGAADMLGLPV
                     STTQVLSSGVAGTMAANQSGLQWNTVRNLLMAWVLTLPASIVLSGSLFWIFRHLV"
     gene            complement(31625..31897)
                     /locus_tag="PK589_09585"
     CDS             complement(31625..31897)
                     /locus_tag="PK589_09585"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ19893.1"
                     /translation="MMGGLCAELPWLLGGSDKDAELYLKRAIAADGRFTNAHLILARL
                     LMRQGRLAEAREHIESVLHAEHPHYPYAWKHTFRPEAERLLNLLSK"
     gene            complement(31963..32148)
                     /locus_tag="PK589_09590"
     CDS             complement(31963..32148)
                     /locus_tag="PK589_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19894.1"
                     /translation="MNIADDLFNDEERKQLAYEAASAMAKRALTIDERSAHAHFLYAA
                     SLGSGEASKGFRKVGLS"
     gene            complement(32352..32732)
                     /locus_tag="PK589_09595"
     CDS             complement(32352..32732)
                     /locus_tag="PK589_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19895.1"
                     /translation="MSVGAGITGVWLFLQGHMAASFLSTIAITQLWRVASEYLRADFR
                     GVGTLSWYQILSLLGVLYAFVLTFWLDTFSPSVPNMRLGLRDIWSPGIILALEALWLG
                     IFFFTGRSRVTASTLTMRVLRDRI"
     gene            complement(32748..33407)
                     /locus_tag="PK589_09600"
     CDS             complement(32748..33407)
                     /locus_tag="PK589_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19896.1"
                     /translation="MGDSTSVIALLIVLYGLLFWWAFRTLPQESWQFVASMPRMKLSD
                     GRWVGTNLTYYGAWTATAVVFAVAMAIVLMQSVAVALDAIALLAVVLLGLCVPASKIL
                     ARLIEGKANTFTVGGASMLGLFLAPMIIDGINLVLNWTGKGALPAAAVLAALSVAYAF
                     GEGTGRLACLSFGCCYGVPLQESGPRLSAWFQQYHIAFVGRTKKAAYEGDLEAVPLIP
                     I"
     gene            complement(33397..34422)
                     /locus_tag="PK589_09605"
     CDS             complement(33397..34422)
                     /locus_tag="PK589_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNJ19897.1"
                     /translation="MSMPHQYIERDSNLVVTERFCGDQWLNWAYRALWENANWFMKAL
                     TSRRTTQALGFFNYDRPFTSTQAAVARFARALSVDLSECLDSSDRLNTPRRLFQRRLR
                     YWQVRPMDDDPRAIVSPADARLLIGSFADDSSIFLKGKFFDYDELLGKHRPQWRRLFQ
                     RGDWAIFRLTPDKYHYNHCPVSGRVVDCYEINGRYHSCNPGAVVVAATPYSKNRRIVT
                     IIDTDVPGGTRVGQVAMIEVVALMIGQIDQCYSSVAYDDPQTMQIGMMLKKGCPKSLY
                     RPGSSTDVLLFEPGRLSFSEDLLRNRFRPGVQSRFSEGFGRPLIETDVKVRSTIAWRL
                     ERGIDGR"
     gene            complement(34419..36821)
                     /locus_tag="PK589_09610"
     CDS             complement(34419..36821)
                     /locus_tag="PK589_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19898.1"
                     /translation="MSRTTALSANSSPYAVESRHVIEREAVEFLANSVTLPFSSSDIT
                     AGSESELQAAVLGRSADVDLAQTIHRSNYLKNIRDRTETGESPRIVIQQLQEFLNDSQ
                     STVWENSWVRFPRNMLNKEANQLLACDLRANRRDPKGTPRTDIDRFLVTEQGEEWVRV
                     PVSYVLRLALAQVIGAIESDVPMLAKEGRRLLDHFLNDNSSPETFSFYVSGMDPARGM
                     GKAIAHETAIRFLLTQLLVQYANRAFGLDETGQHAIVYASPHPPVRQRRLNGLISDSF
                     YRELFVNPCLSGWDQGEDKSRYMGLCHEVLSRSQLNAVVRLKDAGIITRNLVVLPATS
                     NISLANNGTHLSLGSRMLTETLQANGKRFTAVHEKVLGDLVIKIVEHFLPLFVGTYSA
                     APYRMNFRDFHPETALGFLPHELDFAHLRMLWRRWKKKAGLSVFGRSITPLGPPWLDR
                     AISFIFNLRGDCLPDFRLIDYLVCLLSTEQSPALDGIPGNDLRLKRDLGQLGVFDERM
                     SLYLLYKLRAQSVMGFSGFEGRHYSLFWNHTDDFAQAASMQALVTALAFQYIARGEVS
                     HEDIPDDPMTESERRQYLFATAVGVGTVNVREAGPNRFLTRILMKTHKTRASRRYAGH
                     LRVRCEDYRMALVAMLEEDASDLIESFQMQECMRSLRDRLSNQDSSTAGKLTHGILNS
                     VGSPSAFDLSGEEFALAAEGYYRRELRAQYLREACESVREELCAIDQGVSEQATQYRG
                     LVTAMIGTRSAVDAFTEEQPRLLAETADAKTLQRCLGLCIMSIAHARISAGRTEEQPL
                     AS"
     gene            complement(36959..37636)
                     /locus_tag="PK589_09615"
     CDS             complement(36959..37636)
                     /locus_tag="PK589_09615"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNJ19899.1"
                     /translation="MPTIKAIIFDFDGVLFDTEAIHFAVFREVLRSEGIPLTLSRYHA
                     AYVGLSDEACFRAILNDHRIVVPKEDIQQLVRRKTDLVQRAFRASLPRVSGVSEFVSD
                     ASMRYRLAIASGALLQEIVLCLKLGSMLTMFEHITAAGDVREGKPNPAPYLHALHALH
                     GRSPIAANECLVIEDTPHGVEAAHKAGMRCVAVTTTLPADRLAQADLVVPSLHALRLE
                     DLTVQLP"
     gene            37847..38965
                     /locus_tag="PK589_09620"
     CDS             37847..38965
                     /locus_tag="PK589_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19900.1"
                     /translation="MPSADAPSLVIEQGTCYALFAYDIGLAINLDEAERHVTAIKERG
                     RMRHKARAPHYFEYRPAPLRLIQETASLDFGDYRSSEAVEVMLYDFGAVTVIYRIPIN
                     GPFEGLLSLSESLYENERLLADSRHRLDQLVRDIRLAIDRPSVSPEVEDYLVFSVEHC
                     SPSAIQALGTPFEPMFAHVLRSERTRLSDQEIREATSCRISFSRDDTALIDWNAAIVF
                     GEDMDDVRAVLEFVNVELLEMRTLDEQLDQALDQGYVALTRKARVTSWLPGSHEAALT
                     QIGQLQVDSAVLFERVANTLKLLGDQYLARVYRLASQRFHLEAWDASIIRKLQTLDSI
                     YGKMSDRATTQRMEVLEWIIIALITISIAVSFLPMTSH"
     assembly_gap    41680..41779
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(41849..42295)
                     /locus_tag="PK589_09625"
     CDS             complement(41849..42295)
                     /locus_tag="PK589_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ19901.1"
                     /translation="MKSVSLFLYAWTVVGVGAIAWSADSEVLRPRVPVDQIESARAVT
                     NPFPVTPEMREKGKALFEGKAFCRACHGADGKGLGLDLDYSTFKGPLPRNFTDKMWQQ
                     ARTDGELFWILKNGSPGTDMASFIPLVLTEEEAWQVLMYVRSFGGR"
     gene            complement(42380..42934)
                     /locus_tag="PK589_09630"
     CDS             complement(42380..42934)
                     /locus_tag="PK589_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HNJ19902.1"
                     /translation="MRSAIAMIGLCAAALTATGCAETSHDMKSTAAAAPAATAAKAMP
                     TPAQGYTIHVMAPHKFEDGTVHGPYHHYCKPISPEILQCLLFESTDSNALLTDIEYFV
                     AKSVSRAHVPLETWNKYYHDHEVEIATGRVQILDMPDAQAKEVAAVAAKTDGIIFHLW
                     PDGAKAPNGEVGHPQSVGHKHRTE"
     gene            complement(42988..43494)
                     /locus_tag="PK589_09635"
     CDS             complement(42988..43494)
                     /locus_tag="PK589_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HNJ19903.1"
                     /translation="MFRQLCVLGASTLALAMGGPALAADMKPGPADGFDIHVMAPHKM
                     EDGSVAGPFHHYCKAIKPEVLQCLLFESTAPNAVLTDVEYFVAKPVSRSAVPLDVWNK
                     FYHDHEVEIATGRVQVLDMPDAQAKEVAAAAAKTDGIIFHLWPKGAKAPDGSVGHPTS
                     VGHKPRTE"
     gene            complement(43605..44675)
                     /locus_tag="PK589_09640"
     CDS             complement(43605..44675)
                     /locus_tag="PK589_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNJ19904.1"
                     /translation="MVRTVLPLCFILVLLCVATFGIGESLVGASPSGHAPHGTVTIDG
                     ITVPDIGPLPTAVPIPSSNVNYAAKIELGKQLYFDGRLSKNNAISCAFCHNPGTGFAD
                     SRQTSIGVGGGIGGRQSPTVYNTGFNHVQFWDGRARSLEEQAIGPIHNPVEMAETHEH
                     VVAKLGKIKGYQQEFRAVFGTEVNLQGIAEAIAAYERTVLSTNSAFDKYVLGDVKAMD
                     EAAARGLGLFKGKARCILCHNGPNFTDNQFHNLGVPQVGPMKEDLGRYNVTRADKDKG
                     AFKTPTLRSVTETAPYMHDGAFKTLEEVVDFLDQGGGSNPRLSPLMRPLGLTTEEKAD
                     LVMFLKALTGEPIKFSMPQLPK"
     gene            complement(44828..45421)
                     /locus_tag="PK589_09645"
     CDS             complement(44828..45421)
                     /locus_tag="PK589_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19905.1"
                     /translation="MGQAAELDAVIARMTADFLAQNTAAGMLRRALDDVGVGFVPVMD
                     HLTIRTMNIDQRAEEFVALGYGYDETIYYDDWYAKVFRKAGYPALFVDQAYPDARGHT
                     SIIPRWVDKFGDRVFHHVAVRVEDIEQAILKLKSKGVVFAGEIVGARGGPLRQIFTAP
                     EMIDGQPFSVLELAERHQGYQGFLPPQADSLMRSTVR"
     gene            complement(45405..46961)
                     /locus_tag="PK589_09650"
     CDS             complement(45405..46961)
                     /locus_tag="PK589_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNJ19906.1"
                     /translation="MTTEELFTRLGLAREQSGGGGSSSTWTRTTPAGVVESRNPSTGQ
                     TLGRVYGCTDDEYDALVIGLQHMQKVWRTVPAPKRGEVIRRIAEALREQKDALGSLIA
                     LEVGKIKAEGDGEVQEMIDMADFAVGLSRMLYGYTMHSERPQHRMYEQWHPLGVIGVI
                     TAFNFPVAVWAWNAFIAAVTGNTVLWKPSPKAPLCAVAIQQLCNRVLEDAGYPGVFAL
                     CTTDRVELAERLVRDERLPLISFTGSVPVGRQVAEVVGRRLGRSLLELSGNNAIIVDE
                     TADLELATRAIVFGAVGTAGQRCTSTRRVIVQDSQYDKLVSRLVSAYGQVTIGDPLQP
                     NVMMGPLIDREAVARYQSALEDVRQAGGEVLCGGRVLSRPGYYVEPTIVRAQNHWKIV
                     QQETFAPILYVMTYRTLDEAIDMQNAVPQGLSSALFTTHLHHSEAFLSASGSDCGIAN
                     INIGTSGAEIGGAFGGEKHTGGGREAGSDAWKAYMRRQTTTINWGTELQLAQGITFGR
                     SEGGNHGASR"
     gene            complement(46969..>47351)
                     /locus_tag="PK589_09655"
     CDS             complement(46969..>47351)
                     /locus_tag="PK589_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HNJ19907.1"
                     /translation="TLRYPGHCEKIQFLMNDLKLNEDRETLKRILERAIPQTHQDVVL
                     MYAAVTGTRQGELFEETYVKKVYPRMMLGRTWSAIQVTTASSLCCVLDLVLAQPSAYR
                     GFIRQEHFPLRDVVNNRFGDCFRS"
BASE COUNT        10920 a        13734 c        12422 g        10175 t
ORIGIN      
        1 tgagtgatgc ggccctgaag gaagtgaagc gcctgatcaa tgtccaaggc attgaagaag
       61 gcggcctccg cttaggcgtc aaaggcggcg gctgttccgg actgagctac accatcaact
      121 tcgacgaaaa aatcggtcag tacgaccagg tgtacgactt cgacggcgtg aaggtcatcg
      181 tagacgccaa gagcgcgatc tatctgcaag ggactcaact ggactttcag aaagacctga
      241 tgggcggaaa tttcaagttt gtgaatccga atgccaataa aacctgcggt tgcggcgaat
      301 cgttttcagc ctgagcgagc gagtgcagat gagtggacat acccataacg ccgacgaccg
      361 ccgcgaactg caaatggctc gcagcatgtg ctggcattgc cagtctgaaa tggccggcga
      421 atacttttgc gaccggtgcg tcaaagtaca accggtgtcg aaagatctcg actatttcac
      481 ttgcttcggg ctcccccggc gcctcgccat cgatacgcaa gtcctggaat ctaagtttta
      541 cgaattgagc cgggcattcc atcctgattt ctatcagaat aaaagcgatg cggagcagtc
      601 tatcagcctc ggcaactcgg ccatgttgaa caccgcctat cgcacgctac gagatccgat
      661 tcagcgggca gagtatttgc tggatctcga ggccggttcg gtaaaagata tccgtacgtc
      721 accgccagcg gatctgttcg aagaaattct tgaactgcag gagaccctcg acgagttccg
      781 cgagagcgat cgctcatcgg aacacgcgtc gacgctgcgc gcgaaactcc ataccgatcg
      841 aacgaatctc gaggaacggc agcgggatat ggaggccaaa ctgcagcagc tgttcagccg
      901 ctgggatgct ctgcaagatc gcggggaagc cactgagcag gcgcgagctg aacggacgct
      961 tatcctcaaa gacatgcggg acattctttc gaaccgcacc tatgtcaaaa acattgtcaa
     1021 cgatctcgtc gcaacgatcg cgtaacttgt atggcacgca ttgtcggaat cgatctcggt
     1081 accacgaact cgcttgtcgc ctacatggac aagggaaccc cacgcgtcat ttcggcccgc
     1141 cacgaacgtg ccatggttcc ttccgtcgtc gcgctgaccg ataacggact aatcgtcggc
     1201 gatccggcca aggagcatct caccagaaat cctgaacgta cggtctattc cgtcaaacgc
     1261 tttatgggga aaagtctggc tgacgtgcag ggcgaactgg cttactttcc ttacacgctc
     1321 acagaaaagg gcggagtcat ccgaatccag ttgggcgaga aatcatactc cccgccacag
     1381 atttccgcga tgatactcaa ggaactcaag ctccgcgccg aagccttcct cggagaaagc
     1441 atcacgaaag ccgtcatcac cgtgccggct tatttcaacg acagccagcg ccaggccacc
     1501 aaagatgcgg gcatgatcgc ggggctcgaa gtcttgcgca tcatcaatga accgaccgcc
     1561 gcctcacttg cctacggcct tcagaagaaa acacaaggga ccatcgccgt ctacgacctg
     1621 ggcggaggca ccttcgatat ctcgatcctg aagttgaaaa acggcatctt cgaggtcctt
     1681 gccacaaatg gcgacaccca tctcggtggg gacgatttcg atcagttaat tgccgatctg
     1741 ctcctgaagg acattcgcgc gcaacatgga ctcgatctga gcacacaccc agaccatatg
     1801 caggccgtac gcttacaggc tgagcgagcc aagattcgtc tatcagagga gctcaagact
     1861 accgtgagcc ttgatcttcc tgatggacag ggccggttct cccgcgagct cacacgcgac
     1921 cagctcgaag cactctgcat tcctctggtt gagcgctcac tgggcccctg tcgtctggcg
     1981 ctgaaagatt ccggattgaa tccgacagac attgacgaag tcgtgctcgt gggcggatcc
     2041 acgcgcatgc ctctggtccg ccaacgggtt caggctcttt tcggcaaaga accgcattgc
     2101 gaattgaatc cagacgaagt ggtggcactc ggagctgcag tacaagccga tattctgagc
     2161 ggcggcacca ccgatatgct gctgctcgac gtgaccccgc tgtccttagg tatcgaaacc
     2221 atgggcggtg tcatgagcag cttgatccga cgcaatacaa cgattcccgc cagcgccaag
     2281 gaaatgttca cgacctatgt cgacggccaa accggggtgg acatacacat tctccagggc
     2341 gaacgagaac tggcgaagga caatcggagc ctcgcccgct ttcgtctcaa ggttccgcca
     2401 ttgccggcag gagtgcctcg catcgaggtg acgttcctga tcgatgccaa tggtattttg
     2461 aacgtcatgg ccaaggacat gcggaccggt gagacgcagt cgatcgaggt caagccgtcg
     2521 tacggattgt cggatcaaga agtcgaacgc atgatcgaag actcctttaa attcgcggcg
     2581 gacgatgtca gcgcgagaaa agtgatcgaa gccaggctgg atgcgggtgc tctgattacc
     2641 acgaccgaaa agtcattgac tgacggcgga catctcgtcg aggccgaaga aatcgccaag
     2701 attcgcgctg cgctgtctgc cctggcggct gccaaagagg ggacagatcc gcgtgccatt
     2761 cgcgggtgta tggccgacct ggagcaagca gccaaaggcc tgaccgtggc catgctggac
     2821 gattctttga aacaagggct tcaaggcaaa aaaatctctg atgtgacctg attcaccgac
     2881 gcgaggagct ctgatggatt tgaaatggca ggatgcggaa gatatcgcga ttcgcctggt
     2941 cgaggagcat ccggatgctg acccgctgac catccgcttt acggacatgc acgcctggat
     3001 cgtcgccctc ccggatttca aagacgatcc caagaaatca aacgagaaaa tcctcgaatc
     3061 catccagatg gcctggcacg aggagtatca ggactcccgg tcctgatcga ttccgtacgg
     3121 ttgctccatc aacatatcct cacggacgtc gatagaacgc tcgcataact ggctcatcgc
     3181 aggccatgga tcggcctgat cgagataatc tggaggctct tctattgcgg ctcgacggga
     3241 gaaggcgaag cgctctcctg ggcttgatcc agcttgtaga attcggtggg atcgatcttg
     3301 gagccggcac ttttcgtcgg ctccgtgccc ttggtaaaca actccaccgt gccacgctgt
     3361 tcatcttgat cggtgaggag cgccgtcgaa ggatcaacct tgacgaacat gactccctcc
     3421 ggaatttcaa acggcacaac cggcaactgt ttgagcgcct ccttcatgaa ggcgatccaa
     3481 atgggcaggg ctgcatgtgc gcctgactca gtttctccca gggggcgccg atcgtcgaac
     3541 cctacccacg ctccggtggc taaattgggc gtcgaaccga tgaaccaggc atcggtaaag
     3601 tcgttcgtgg tgccggtttt tcccgccacg ggcctgtcaa tgaccgcttt cgccgcaatg
     3661 cccgtgccgc gctggatcac atcctccatc atgttcgtaa tgaggtaggc tgtttcacga
     3721 gataccacct gccgcggctg aatggcggcc tgctccaacg tgcgtccccc gctatcctgc
     3781 acgctggcca cggcataggg ctcgacacgc acgccttgat tggcaaatac cccatagact
     3841 gaaaccagtt ccatcaagcc gacactggaa gacccaagcg ccagcgacag gtccgcggcg
     3901 agcgggctca cgacgcccac ggtgcgcgaa aaatcgatca cgtttcgaat cccgacctta
     3961 tccagcagcc gaaccgtggc gagattgtgc gaatggatca atgcttcccg caagctgacg
     4021 atgccgtgaa aacgcttgcc gtaattttcc ggcttccacg ttttgtcctc ttcctcttgc
     4081 tcatagacca ccggcgcatc caacaccacc gtggccggac tcatgccctg gttcatcgcc
     4141 gtcgcataga taatcggttt gaatgaggac cccggctgac ggcgcgcaag cacggcacga
     4201 ttgtattcgc tacgcgcaaa atcatatccg ccgaccatgg cgcgaatggc gccggttttg
     4261 ggatcgacag ccacgagcgc gccttcgacg acaggcgtct gctccaaccg cagatgcacg
     4321 acatccttat caagcttctt gacgcccact tcgatcacat cgccgggcga aaggagttgc
     4381 ttgatgttct tcactggaac ggcgtctttt gtgggatctt ttcctttgag atatttggag
     4441 gcccaggcca tatcgtcgaa gctgagccgg cccgttgtcc cgccaatctg gaccagcaca
     4501 tgatccttcg cgattttcgt caccacccct tccatcatat cgccctcggc gagcttcaca
     4561 gagggatcgg gtgccggaac ttccagggtg gtcacatcga cggtgcggag cgggccgcgc
     4621 caaccctggc gcttgtccac ttcacgcagt ccgttgaaca cggcagcttc tgccgctttc
     4681 tgcatgtcgg cattcagagt ggtaaagact tccaatccgc ccttgtagac catggcttcg
     4741 ccgaacttgg ccacgaggag ctgacggatg tattccacaa agtacgggcc aagatgttcg
     4801 ctgccgggac ggcggaaatt cagcttttcg gcaatggcct gatcccggtc ggcgacactg
     4861 atgaaccccg cttccgccat gcggctgagc acatgttcct gccgcttctt cgcacgatca
     4921 taggctttga acggtgagta gtggctgggt gatttgggaa gccccgcgag gaacgccgat
     4981 tcagctaatg tgagtttcga aagttctttc ccgaagtagg tctgcccggc cgccgctaca
     5041 ccgtacgcgc cctggccgaa atagatctgg ttcagataca tctccagaat ctgttccttc
     5101 gtcagcacca gctccatttt gtaggccagg atgagctcgc gcactttgcg atcgaacgtc
     5161 cgctcggccg acagaaagag cgaccgcgcc agctgttggg tgatcgtgct ggcgccctcc
     5221 acttttttgc cgccgtggcg caagttcgtc caggccgcgc gaagaatgcc cacgatgtcc
     5281 aggccggggt gttcgaaaaa ccgcgtatct tcggtcgcaa tgacggcttg cgtcaggctc
     5341 ttcggcattt cctggatggg tttcagaaag cgtcgttcaa tgaagaactg cccgatgacc
     5401 tgccggtcgt cggaataaac acgcgtgacc aaactcggct gataattctg gagtggatcg
     5461 agcgaaggca gatcttgaga aaaataccag agagtccccg cggcccctcc cccgccaagg
     5521 acaatggcga gcagcaggcc gatgaggata atcttccacc accgccgctg acgacgtggc
     5581 ttttccggct tatcgagcaa atgatcgagt tcggacatga agttggatgg ttgcgaggaa
     5641 agaggtcaac ccgcgacaac cccaccatac aatgcgcacc ggacaaactc aagcaaggcg
     5701 cgaatcgtcg tcgaaagaag tttcctttcc acattccaca atgagcttgc gggaagtcgt
     5761 ttgaatcagg tatagtccat aggcttagac gccttcgtag gaaggcgtct tttttttggc
     5821 actttcacgg cattggagat tatgaacagc tcagcgccct catccacctc aggtttgcac
     5881 gcgccgaaag gccgccggac cgacattcgt aacatcgcca tcatcgcgca cgtcgaccac
     5941 gggaaaacca cgctcgtcga tgcggtgctg cggcagaccc acgtgcatcg taagatcgat
     6001 gacatgggcg aacgcatcat ggactcgatg gatcaggaac gcgagcgcgg catcaccatc
     6061 cgtgccaaga atgcgagcgt catttataaa ggcgtgaaga tcaacattgt cgatacgccg
     6121 ggacatgccg atttcggcgg tgaagtcgag cgcacactcc gcatggtgga tggcgtgctg
     6181 atcctcgtcg atgcgaaaga aggccccatg ccgcagacca cgtttgtctt gcgaaaagcg
     6241 ttggccctcg gacataaagc gatcgtggtc gtcaacaaga tcgatcgccc tgacgccgtg
     6301 atcgacgatg tcgtcaaccg gacgttcgac ttattcgtgc atttgggcgc caacgacgaa
     6361 caactcgatt tccccattgt ctacgcctca gccatcaagg gcatcgcgac ccttgatctc
     6421 aaacagccgg ggacggacat ctctccgctg ctggaaacca ttctcgacaa aatccctgca
     6481 ccggccatta accgcgacga tccgttccaa ctcctggtac tggctcttgc gcaggattcg
     6541 tataaaggga aaatgggaat cggcaagatt caatccggct cgatcgcccg acgtcaaaat
     6601 gtggtgacgc tcaccaagga cggcggccag gttcccggca agatttctga ccttgcggtg
     6661 ttctccggcc ttgaacgcag tgacgtggat tcggctgaag ccggtgaaat cgtcgccatt
     6721 tcaggactgg aagaagtgaa catcggcgat acgattgccg atccgggcag tcccgtcgcg
     6781 cttccgcgcg tcacgatcga tgaaccgacc gtgcaaatga ccttttccgt gaacaatagt
     6841 cccttcgccg gccgcgacgg caaatacttg acgtcacgcc atctgcgcga acgattgttc
     6901 aaggaattgg aaaccaacgt ttcgttgcgt gtgcaggaaa ccgatagcgc cgaccgattc
     6961 ctggtggcag gacgtggaga acttcatctt ggtgtgctca tcgaacagat gcggcgcgaa
     7021 gggtatgaac ttcaaatctc ccagcccgaa gtcattctgc atcgcgatgg ggagaccgtc
     7081 acggaaccgt ttgaagaatt gaccatccag gtcccgtcgg aatatcaggg tccggtgatc
     7141 gaggaaatcg gaaaacggcg cggtgaactc cgccacatga aactcgtgaa ttccgaaggc
     7201 accgccagcg aaatgcatat cgagtatcac atcccgacac gcggcatcat cggcctcaag
     7261 aacattctgc ttgcgaagac gcgaggcacg atcattatgc accacgtttt ttcaggctat
     7321 gctccagcgg acgaaaaggc gttgcttgtg gcgccccatg gctcactggt ggccttcgaa
     7381 gctggaacca gcactgcata tgcgctgttc atgactcagg agcgcggcga actgttcatt
     7441 ggcgcgtcgg ttgaggtcta tcagggaatg gtcgtcgggc aaaacagccg cgacgaagat
     7501 ctagacgtca atgtctgcaa gcagaaacag ttgagcaaca tgcgcgccgc cggcaccgac
     7561 gaagcgctgg tgctcacgcc gccacgggaa atgtcgctcg aattcgccat ggagtatatc
     7621 ggccaggacg aattggtcga ggtcacaccg aaatatttgc gccttcgcaa gcggatgctg
     7681 aacccggacg accgccggaa ggccaagaag aacgcgaagt agcgtctaac gattcccgcc
     7741 ctgctcgtgc ggactgcatg aagattcgga aaaaggcccc gaaaccttct cccgcaaagc
     7801 ctccgctcta cgtgatcgga tatcgaggga tgccgccgtc cctcggtgaa ctgaaggtct
     7861 ggtacgacct ccactacgga gggcccctga cctggctgga accggtcgca gaaggccgtg
     7921 ccagtgcgag ccacggtccc tggcgggcac atgtgataac cagtctcccg gataccgaaa
     7981 ctgcacaatg gcagcaggtc ctgtcatggg atcatcaaga actgggtgcc gtttctctct
     8041 cctccgccac gccaagcacc atcgcagaca ctgtgttggt cactgcccga ctcgcccgcg
     8101 gcctgacgct gctcacccaa gggacagcct tcgatgtggc ctcccatgaa tacctgaatc
     8161 cttcggattg gaacgaccgc ccgctggatg tgtttgtcac ctccgaccat gtcaccgtcc
     8221 agcacggcga gaccgacgat cattcttccg aatggtttta tacactcggt cttacaaaat
     8281 ttggcttgga cgaattggaa gtgatccagc cgcgaggtct cccggagcga gaaaccatcg
     8341 cgctgttgcg ctgtgccgcc gacgccgttt tgcggaaggg gcaaaaccag aaggtagggg
     8401 gcacaatgga tctccatgcc gtcgctcaca ccattcgatt cattaagcat cgcaccgccg
     8461 caccaaccgg ccgcatgatg gcgttccgac aaatcagtac cgaccttctg tgacctgctt
     8521 tagttctccc ctaagccatt gcgaaaccaa tcgattgtcg ccgttcaccc tctccgtcta
     8581 ggccttctgg ccccgttgac aaggtttttc caggcgtggt accacgaatg agtttgcgca
     8641 gttccgcagg tctaacctag gaggatttca atgagtgatg tggctgcaga aattaaagtc
     8701 ggcgataccg cgccggattt tacattaaag gatcaggatc agaaggacgt caagctgagc
     8761 gattatcggg ggaagagcaa tgtggtgctc gcgttttacc cgctcgactg gagcccggtc
     8821 tgtcagggcg agaacaagtg cctgaccgat gacttcccca agttccaggg cgcccatgcc
     8881 gaactgttcg gcatcagctg cgacagcttc ttttcacaca aggcctgggc ggattcgctc
     8941 gacctgaagc accgcctctt gtccgacttc aaccgcgaag tggtcaagaa gtacggattg
     9001 tattttgagc cgttgaattg cggcaagcgc gccacagtca tcgtcgacaa gaatggcaaa
     9061 gtggcgtacg tcaaggttca ggaaatcaag gtcgcgcggg aagataagga cattctcgcc
     9121 gccctcgcga aactgagcta agcgataaac ggggaccgag gggatagggc cataacgagg
     9181 ccctcccctc gatcatctgt cctagacgag gagagcccat gaccggcaca gtccaagatg
     9241 ttcgggatga gaactataaa gaattcaccg agagtgccgg tgcagtggtg gcctatgggc
     9301 tggccacctg cgagccctgc aagcagtacg atcccatctt ggaagaaacc gccgcaaaat
     9361 ttcccaccat caagatcggc aaggccaaga tgcatgtccc cggccgttgc cgcgagatca
     9421 agaaagcgca tacgtttgaa acgtacccca ccacacactt cttcgcgcag ggcaaactgt
     9481 tgctgactcg cgaaggagtc gtcgagccgg ccgagctggc cgcgttaatc tccgaccatc
     9541 tgctgaagta aactaccctc gcggccacac ccagctccga tcgcaggtcg tcgacgcgac
     9601 aatacagctc cccgcgttcc atccatccca cttcccgacg cagacgcgat acaatagact
     9661 tgtaacaagc cgctgtctga atggaatgac tgtggattag ccttcaaccg atgcgcggat
     9721 tggctttgct ctcctatgtt ttcacctggc tgctctggct catgattcct gaggccagcc
     9781 tcggctatga gaccccgcca agtgcctttc ccgtcagcca aacggaattc cacgccgtca
     9841 caatcaattc ttcaaagagc gctcccatgg ccttcatccc cgcgggcgag ttcgccatgg
     9901 gaagtgatcg cggccaggac gacgaacagc cggtacatcg cgtcttggtc aaggctttct
     9961 atctggagac acacgaagtg acagtcgctc ggtatgccgc gtttctcatg tcacaaaagg
    10021 cagacccgcc cttcaaatgg aatgaggcga ccagcggtgc ccacgagagc aaaccggtgg
    10081 ccggcgtcaa ttggtatgac gcgcgcgact actgccggtg gatcgggaga cgattgccta
    10141 cggaagcgga atgggagctg gctgcacggg gcaccgaggg acggatgtac ccctggggag
    10201 atgctcagcc cacgaggagc cacgccaacg ccgggcacac taaatggcat ggctacgaca
    10261 cactgaccaa cgtagggcaa tttgaattgg ggaaaacgcc gaacggggtg tacgacctgg
    10321 ccgggaatct atgggagtgg gtcgccgatt ggtacgacgc cacctactac cagtttagcc
    10381 agagagaaaa cccttccggc ccctcagcag gccccctcag ggcattacgc ggcggggctt
    10441 ggaacaatga tgccaagacc attcgatccg ccaatcgcgc cgggtatgcg cccgatgccc
    10501 gaagaaatga tgtcgggttt cgctgcgcca aggatgcgcc gccatcgaac gcgcgctaga
    10561 agaggcggga actgaaggca ggagattcaa gctgccgcat ggcttgactc atttcccccg
    10621 gtcgatcccc ggagcgtaaa cggatcttaa agaccacccg gcggcttttg tcccgatcga
    10681 gcgcccggga gtgatccatc accagatcct gccggcctcg tccactggcg gacgtctttt
    10741 catgaaagca gcgataggcc cagggcgccg agtctttaat cacctcaagc cccttcacca
    10801 tcacatttag tgatcgttcc tggctgagct ttaaattgta gatgtccgac ccgcgatcat
    10861 cggtatgccc ttcgatgacc agcgagtcga tacggtcccg catggcgcca cagagaagcg
    10921 ccgcgtaggt gggcatcgca tccaccagaa accgatccgc gacggacgac agcgttccct
    10981 taccaaattc gaagttcaac aaggcatcgg ggacgacaat tcgtacgata ttgggatcag
    11041 tcgggtcggc atccagcgac agatcgaacc gctgaaagtg atcgcgaagc attcccttca
    11101 tatcctcggt tgtgctcgtc gcgacgttcc gctcgggcgc ctgaacagga gccttcactt
    11161 tcgtttgtgg ttccgtttcg gacaccttgg tcacgtaggc gctgaacagc aggataaaaa
    11221 tcacggccag cgacgtcatc aggtctgtga ccccgattgt caggaccgaa gactgctcgg
    11281 tggattccgg cgaatgattg gcacgcatgt caggccgccc ccttcataga ggccccttgt
    11341 cgccaattcg caaaccaccg taacccgagc atgctatcat caggctcgac ctcctgttgg
    11401 atatcctgct cgctctccgc agtcggcacc tcgtccaacg agtcgaattc aaactcgtcc
    11461 gaaacctctt tgacgcctcg atcctgcttc agttcttcta ccgatcgagc gagtgtctga
    11521 atagcctgat tcaattcctg aatcggcgcg gccatggtct gttgcatcac ccgagagagt
    11581 tcctccgcca gacgatccgg agatcctgta cgtcctccgg ggtcacgcct gctcagcact
    11641 tctaccgctt ccctcagagc ggcaaccgtc ggactgagtc gatcgctgag gctcccggcc
    11701 agacgatcac cgaggtcacc gggcaccgcg cccacggcag caggtgactg tgccggcgtc
    11761 ggaccggctc cgggcgtgaa gttttccaac atctgttcca tggttttgcg gggaaacaat
    11821 tcgtccacca tcgtgacgaa ttgctgttga gtcgtcgcca atcgatgcat cgccgacttc
    11881 tcaagcaaca cgaacagatt ggcgcagagc aatcccacga tcgacgtgag aaactttcca
    11941 gccaatccgt tgatcaaccc ctgaatcccc acgatatggg atccgtcggc atggagctta
    12001 cttaaaccga tcaggatggc cagaaacgtg agcagtaaac cgccgcccgt gatcaatgat
    12061 ggaaattgat gataaaacgc cagattgagg cggctgttca gcaagtcgcg ggggaaaaat
    12121 tcggctgccg ttcgtgtggc aaacactttc ggttcgataa accaggctgt acgttcgacg
    12181 acgaaggtct tgcgaaagtg caaccaggcc tgttcaaggc gcggctcacg gcgcatggcc
    12241 ttgtccagcg tatggagatc gtccagatca atgcgctcgg gttgggcgcc ggattggttg
    12301 gcgactctct tcacatcgct caaactgggc accgtcagcc actcgttctg aaggcttttt
    12361 cgactggccg ccagacccgt caaggtcggc cagacacgcg tataacaggt ctgcacgctg
    12421 gaaatggccg tcgagagccg cccggcgtgc cacatgaaca atgcgatcag cccaccagac
    12481 ccgagccaac ttaacatcgg gctatggagc tctcccagga gagtcacgtc ctggcttaaa
    12541 aattcccaga gagacatcat ggcgcagcac ctcccgctcg aaacacgcat ggcccactgc
    12601 ttcagaccat gtgtgttatc aagtttcggg ccaggatgtc tgttgaagaa aaacccaata
    12661 acgctgagaa tttgatctgc cggactctca cagaacgatg ctcgagacga atcggctcgg
    12721 caagaattgc cgacctgatg aatctttttg cagtgctctg ttccccttag cttccttcgt
    12781 ctactgacgg accaaggccg agttgtgccc gcaacagatc gtggcgggtg atggatcgaa
    12841 ccactttccc gtcttgcacg acgggaagga tttgaaatcc catctcgaac atgcgcgcga
    12901 cacaacccag cgacgtatcc tctttgactc ccacaaatgc cgaattcatg atgtctccgg
    12961 ctctcgcctg cttcagatcc atgcccttat cgatcgcacg gatcacatcg ccctcattga
    13021 tgaacccgag aaattcgtaa tcttctccta ccaccggggc gccaccgaca tgactggtca
    13081 ccatttcaat ggccacatcc atcgcgctat cccctggctt gaattgccgg atcgtggtgc
    13141 cgacaatatc ccccacgact tgatatccct ccgccggatt gcccgcgacg cctgtaataa
    13201 ccttcatgat cgcctccctt gttactcgga cgaatcgtcg tttccggcaa tctaatgcct
    13261 ttcattcgag acgcgacact ggggagatcc ctatacgccg ccatcgtgct gcgaccgtat
    13321 agctttcaac atcatggcta tgtcatgaat agagcgagtg ggctctttca gtcagacaca
    13381 caacacccat gactataccc tggaccacag ctctccaagt tgccagaaag cttctgccgg
    13441 tggtcattga gaaggccccg gagttgatca ggactttcga acggtttcgt ggggctcccc
    13501 cggcggcgga gccttccgct tccgattccg cactctcagc acttcagcta cagatcgacg
    13561 ctcagcaacg taccattgag cagcaagcgg acacgatcgt cgagctccgt gccacagtca
    13621 gtagaataca gcgcgcgttg accctcgcta gaaccttcct tgccgcgacg atttcgctca
    13681 gccttgccat aatcgcctac ttacttctcc ggccctaatt gcgttcgata gcataggtta
    13741 cgagccacac cgacgctctg atcgagccct ggtgcaggat gaatgtcgat gtaaggagca
    13801 gcgaagcgga agagaggtag tgggaggaac gtgacctagc ccgcgtattc gtagaagaga
    13861 tcgcgggttt gaacgagttt cacgagctga agactgccgg caggaacggc cgacgcgata
    13921 cgatcccata cagccttggc aaggtattcc cctgtgacgg acggcgtccc taacaccacg
    13981 cgaagatctc gtccctcgaa tggaaccaac acgcgttcct ttaccagccg atcaagcgcg
    14041 acgatatcgg tcaccatccc ggtctcagga tgaatcggcc catgcaccga cacgaacagg
    14101 tcccaggtat ggcctcgatg gccctcgtgc acggccgtaa atgaataccg tctggtcaca
    14161 gaggccacat ccaggccggc ctctgccgtg aggtctgcac aaagatcctc atcctcatac
    14221 agagacaacc gctgaagcgt accgatgtcc tgctgggctt gcagcttatc ccagagaaca
    14281 cgcgcgaggt tttcggaagt gggaatctgg tgattgaagt agggcaggtc caggttcaga
    14341 tgcttgtgat cgaattcctc cagcacctgc agcaacacgc gcttcagatc aaagaggttc
    14401 acaaccatgc cggttcgctg atcaacctct cccgcgacgg tcacttccag catatagtta
    14461 tgcccgtgcc cagggtcatt attgcacgcg ccgaaggtcg cacggttctt ttcggcatcc
    14521 cactccggct tgtgatagcg gtgggaagca caaaactcga tgcgcttatt caggaggacg
    14581 gaagccatag tctcacatac accggatgtc actgctgtct gttatgtaca agagttcctc
    14641 gttgaatgcc agtctaaaac tcagaccacc cgctcgctag ccgcggagat cagccaacca
    14701 ttcctgccgc atcgtccagg gcacctcggc atccgcctcg tatccggcat ggcgaacaga
    14761 gagcgccgca cgcacagccg gatcggccag ggccgtcacc atggcaggag tcggacgaaa
    14821 ccgcacgaaa tgcaccgcgc tgattttcgt ttcatggctg tgcccgcctt cgaactcacc
    14881 gtagacgatc tctcctccgg ctcgcagccc aagcttctga ccatggtcca gccccataaa
    14941 cagatcaagc caatgtttca tggtctccgc atccgtcagt tcgatcaaga gggttgcgct
    15001 caattcaccg ggagccggca gcagggcatt gtagacgtct acttcgtcct gcactttttg
    15061 cggatcgagg attcgctcca cgcggatcat ttcctggatc tggaagcgga gcgtctcccg
    15121 gttctcgaaa accatcgtga tcttatcacc gatactaatc cgccgtcggc gtttgagatc
    15181 gataatggtc tgccggtacg cctcgcgttg ccgctcgtac tcgtccggtg gaagtaaatc
    15241 tgcttgtgtc agttggttca tgtgctatcc ggccaacact ggctcagtta cgaagtccgt
    15301 ggatctctgg gcaacccata ggcgtcgcgc accacttgta tgggatgctg cgccgacttc
    15361 ccgccgacat gggcttgagc gccggcttga tccagctgaa ggcctgcgag cggacaatcg
    15421 gatgccacca catcggctgg aacctgctcg acctgacgaa cggctttctg cgcaatcttc
    15481 atcgagagcg gaaaaaattc tgttttggcc gaccatgacc cgtcatggcc ggagcatttt
    15541 tcaatcactt cgacctgcgc gccggcgcat tccatcagct cctttgattt gaaaccgata
    15601 ttttggtctc gcaagtgaca gggcacctga taggccaccc gtccgggctt ctggataaaa
    15661 tccatcgcga ggctgccttc tcgcttaagc ttcatgaggt actcacagac atcgtaggtg
    15721 cgccctgcaa cccgtgtggc atcttctccg gaaacgaggt tcgcatactc gcgcttcacc
    15781 atgagactgc agctgggagt gggaatcacc acgtcatagc cttgatcgac ccagggcttc
    15841 aaagatgcga tggtatgttc agccgccttc accatcgcgg tcgtatcacc gatatcaaag
    15901 gagggcatcc cgcaacagga ctgatctggc agcaccacgt gaataccgtt tttttccagc
    15961 acctgcacgg tcgccttgcc gacgtcggtt gcctggtagt tgacgagaca actagcgaac
    16021 aaggcgactt ttttgccagt ggcccctgca ttccgggacg gccgcctgcc ccaccaccgt
    16081 gcgaatgttt cttgttgata gtgcagcacc tgccgctctt ggtgcacacc cagcaccatt
    16141 tgcatgaggc ttctcaccca cttctgaccc agcccccagt tgacgagcgg tgcgagggcg
    16201 ctgttgagtc gtcccaaaag gtcggttctg gtcaataacc aatcccgcca tctaattccc
    16261 ctctcagcgg ccaaccgttt tttccatacg gccatcaaga gcggaaaatc gatcgcgtat
    16321 tgatgcggtg gggtataggg gcaatggttg tagcagagtt tgcaataata acattcatcg
    16381 acgacgcgat ggtgatcggc cggcgtaaac ttcgtgacat cactctcgta gtcatcgatc
    16441 cggtccagaa gcgtgttgaa ggacggacaa agattgaagc agcggcggca tccgtcgcag
    16501 acatcataga ttcgcagcgt ttcttgttcg agggtggccg gatcgatcgg ctggagtagg
    16561 cgaagttctt tcacggaagg gatttcctta tgacgctggc ccagagattt cgacaggggg
    16621 gtgaggcgga acgctccgcc ccacccgatc gtgatgcgcc taaaacctgc aagcaggcta
    16681 ctactgcttc aggctatcga ggcccttggt aaaccggttg gcatgtgacc gctcggcttt
    16741 agctaacgtc tcgaaccatt ccgccaattc cgagaaccct tcgtcacgcg ccgtcttggc
    16801 catccccgga tacatctggg tgtattcgta ggtttcgccc tcgatcgcgg atttcaaatt
    16861 ggcttcggtg ttgccaatcg gcacgccggt ggcgggatcg ccgacttctt tgaggaaatc
    16921 caaatgccca aatgcgtggc cggtctctgc ctcggacgtg tcacggaaca gcccaccgac
    16981 atcgggatac ccttcgatat cggctcggcg cgcaaaatac aggtaccgac ggttggcttg
    17041 cgactcgccg gcaaacgcgt gcttcaagtt ttcatgactc ttcgtgccct tcaaactgtt
    17101 tcccatactg tcctccttca ataaataacg tggcctagct gcgacgtgga gacacgttgc
    17161 gctctgcctg ctgacaactc acacaatatc catgaaactc gactcgatgc ccctggacct
    17221 caaatcccct cgcctgcccg ctcgacagat gtaactgatt caattcgtcg tccatcacgt
    17281 ctagaatctg gccgcatccc agacagatca gatggtgatg catactgacg ttcccatcat
    17341 accgggccat gtcatgaccc acgttgactt cccgaatcaa gccggcgttt cgcaacacgc
    17401 cgagcgtgta atagacggta ttacgcgaca tcatcggctg ttgacgtgta accatccgat
    17461 agagatcatc ggcactcgga tggctcgtcg tctcgaccaa cgccgcataa atcgctgcgc
    17521 gctggcgcgt gacacgcacc gcatgccggc gaaaacgggt ctcgatttcc cgcagatcta
    17581 tgctcatgtc attgttcact agacaggaat gattcctaca tagcacggag gtctgccgaa
    17641 cagcaagaca gaagcaggcc ttttcaaagg cgtatcaccg ccagcgctgg agctatttga
    17701 aagtgaagcc gtgaaggagc agcagacgag atgatgcgtc agatcgaatc gatcaaggga
    17761 atcatcctag cgaagtggga gcagcaaccc tcggctagga ggcctcgtac cgtggcaact
    17821 ccgccgtcat gtcggcgagg catttgtagg cttggtcttt tgctgaaagg agtggggcgg
    17881 tgatgggcca ggcgattgcc aatttcgggt cgttccagat gataccccgt tcatccggcg
    17941 gcgaatacac atccgtgcac ttatacagaa acccggcttc ctcgctgagc acgcaaaacc
    18001 catgtgcaaa gcccggcggg atgtacagtt gccgcatgtt cgcctccgac aattccaccc
    18061 cgtatgattg tccgaaagtg ggtgaacctt gccgaatgtc gaccgccacg tcatacaccg
    18121 cccctttcac caccatgacc agtttcccct ggggtttggc caattggtag tgcaggcctc
    18181 tcaacgtgcc gcgaacggac caggaaaagt tatcctgcac gaagggcttg gtgattccgg
    18241 ccgctccata ccgcgactca cgaaaaattt ccacaaactt tccacgcgca tccccgaaga
    18301 catccggctc gatctgaaac aggccgctga gcggcgtcgc tgtaatctgc atgacccctc
    18361 ttgaatggaa ggacgttcgt caggcgcatc ataccctctt cttctcatgg ggaaaagctg
    18421 atcgcgtgtt gcgcacgcca agggcgggag gcgacgaaac ctatgcgact aattgcgggt
    18481 ggaaggatcc gatgtcggtg gaggatcggg atagagtttc tgcaaacggt caatcagcgg
    18541 ttctgcggca ggactgctgg ggcgcgtggg ctcgctgggc ggatgatccc aggtcaaagt
    18601 cgaaatgccc gcctccataa agagctgtcg aatcgtcgca accagggcgt ccagcgtgag
    18661 ctctgtgccg gatcgcaagt ccagcacccg ttcccgctcg atggtcggtg gaggatcagt
    18721 gctgaccagc gcccaacagg catcaaacac cttgcggcgg aggtcttccg gcacattcaa
    18781 cgtttcaaga cctgacgtgc acaaggccga gactaccaac acaaattgaa ggtcgggcat
    18841 ccccggacgc tggatattga gggattgcat aatggtaata ttagtcgcgc ccgcgagggg
    18901 gagtcaattt gcatccacac ccaagcggcg cccccaggaa cagttgcata tcattaagag
    18961 gataggcgtc gcatgattac gattacgctg atgggacaac ttcaaacgac ggatggggaa
    19021 cgcgacctgg cttgcgagtt gccgactccc gtcacggttc ggcaagtaat tcaacggcag
    19081 ggccgcggcc ttcggcacat gctccaactc ctgcgggaga agaaggtgct cgtgaccatc
    19141 aacaaacgca tcgccagcga agattcgatg gtccaggatg gagacgcggt gcggttcgtg
    19201 ggacatgacg gggccggagg aagcggcttg gctccgtcat tctaagacta gcgtgcagca
    19261 ctgagcgccg gtccgtggcg gctgctgata atgcaagggc cggtgcctta cagaaagcac
    19321 cggccctgga tcgacagagg aacgagatct attacttctt tccgaggatc gaatctttgg
    19381 ccatcttcgc gacccggaac ttcaccacgc gcttggcggg aatcttgatg ggctcaccgg
    19441 tctgcggatt ccggcccatg cgcgccttcc gattggcaag caccagcttc ccgatgccgg
    19501 gaaccacgaa ggcgttcttc gcttcgcgat aggcgagggc ggcgaaatcg tccagaaatt
    19561 ggacggccgt tttcttcgta attcccgcct ttccagcaag atggtcagca atctgcgatt
    19621 tggtcatcga cttggccatg cgttcctccc tttgaacaac gaatgaaagt gaactgacgt
    19681 gtgcggtctg cgatctataa actcgtacgt acagaggtgg agccggaaat ctgcgcgtgg
    19741 tctgcagaat cagtccgaaa acagggcgag agtgtatcca aagccctagg ggctgtcaag
    19801 agccgacaga tggaaacatg cggcccagac tggcgacacg cgcgtttgac gcccttgcgc
    19861 gccaagcaga tcacagagta cagtacacgc acgcatcgcg gcgcagcgtc atacttagga
    19921 acaagaggcg ttttcatggc caaagaactt cccattctcc cctcgaatgc ccaacctgcg
    19981 gaaggcaccg cacctgaagc agtaatctac tcccgggtct tttgtaaaaa tgagaatgcc
    20041 cctccccttc gtctgttgat cgaattttta aagtcgcgcg ggcaatcgcc gatccttccc
    20101 cccgacatgg atgattccat gctcgatgaa tgggcctggg tgcaggtgat tctcggctat
    20161 gcacgcgaac gcaaaccaat tcaggtgttt tgcatccggg accgcgggac gtatcaggat
    20221 ctcttcgaac aggagcagaa acagtttcga gaagacctgt cctcccatga cgatctggaa
    20281 gccacgctcg cgcaggaaca tgtcacgcgc gcgcgcttca ttctaacaac acgcatgatt
    20341 gaagcggacg tgacggagga aggctacgat ttcaacggct ggatcctgga gtttttccag
    20401 gaacactgca acgggatcgt gcagatcgat caacaagggt ttttttcgcc aaagggagaa
    20461 ctcatcgtcg atctttcgct gcctgaagag tagcctgatg gggaccgatg catccgcccc
    20521 tgccgctgtt tcagcaagcc tcacactggt tccgacgcgc tcaggcctcg ttgcttgagg
    20581 ccctcccctg cagtcaaggc tgctgcgact gctgtatcgg catcttcccc attacacagc
    20641 tcgatgtcct ggaattgcac cggggcctcg acctgttacc ctccgaccgc aaagccgcca
    20701 ttgttatgcg cgctcgagaa caagtcgccg cacttgaact ggcgttccca gaccttcgat
    20761 ccacaccgaa tcttgatgag tggacggata gtaacatcga tgccatcgtt gaacgctttg
    20821 cagaccttcc ctgtcctgcc ctggcggaag acggacggtg cagcgtctat gcattcagac
    20881 ccatcacgtg ccgcacgatg ggtgtgccta tcgaatctga tggtatggtc cagggcgcct
    20941 gtcgggtcca gaccgccgtc cccattatcc ggccctcccc cagcttacga acggattatg
    21001 acctactggc caaggatgag gccgtagcgt tatcgattct gcggcaagct catcgggagg
    21061 ctggcgacga actgctcctt ccctacggat tcatctagct cacgcatcaa ctccctagac
    21121 agaaacagaa acgctatgct aaggtgcctg gtctgaccga cgggagcgcc tgtagctcag
    21181 ctggatagag catcagcctc cggagctgag ggccacaggt tcaaatcctg tcaggcgcac
    21241 caatcgctct gtcaggcacg tcacgatcga tacatatgca ctccggtggg gccgttagct
    21301 cagttggtag agcagctgac tcttaatcag cgggccgtag gttcgacccc tacacggccc
    21361 accatttttt caagcactca ggtaattcac accttcttca agacctgccg gctgtggcgc
    21421 tggttccaag gcaatctagg atcaccactt catcccgcaa actctccata gcgttgacgc
    21481 tatttcaagc cccgatcacc ttgagggcaa tctatacgaa cttgcatgtt cccgaaggag
    21541 cctcgaactc tggctctata gcacttcaga accccacaac gaatgttcaa acgaacatag
    21601 gtaatacaac agcggctcgg ctgaaatgct cgccttcgac catttgcact gtgatcctgg
    21661 agataatcgc atccggcgca cgcgacaagt tcctttccct tccgctccta gtgttcaaat
    21721 acgcccccac acttttcctc gtttcgcgag cctcgtcaca tcctccctgc ctcccggtct
    21781 cgctgccgat ccgatctccc gggccctgaa atatcgatgg catgtgatga agactcgcaa
    21841 tacaaagaga ataaaatttt ccgtttctta acaccagaca gagcacaaat gcccttcagt
    21901 gcactacaat accacccctc ctgaatacgg gtggcacgat catcccagcc gagtattgtt
    21961 ggccaccggg cggagggttc gagacctacg ggttgtgtaa gtgggaggct ttagatgcag
    22021 gtatttccaa atcgcatttt gttcgtcggg tttggggctg tcgcgcgatg cacattgccc
    22081 attttgctga aacacatagg cgtcgatccg aaacgcatta ctatcttgga ctttgatccc
    22141 aatgaggcag cgttggcccc ttggatcaaa caaggtgtga cgtttgtcaa agatcgcgtg
    22201 acgccagaga atctgggcac gctactaggc aaacacgcgt ctcgagggga ccttctcatc
    22261 gacttggctt ggaatatcga ttgctgcgaa atcgtacagt ggtgtcacga cagaggtgtg
    22321 ctgtacatca acacatcagt agagatttgg gatccgtatt gcggagtaga cgacaaacat
    22381 cctactgagc ggacattgta ctggcgccat atgaatctcc gtcgcatgat ggccagttgg
    22441 ggcgagcctg ggcccacggc ggtcgtcgaa catggggcaa accccggact gattagtcac
    22501 ttcaccaagc atgcgcttct ggacatagcc cggcagaccc tggcagataa gaaattcagt
    22561 ggggagcagg cagagagaat cgcgcatcat gcccaggcgc aggagtttaa ccatctcgcc
    22621 caccagctcg gggtccgagt cattcattgc agtgagcgcg atacgcaaat cactgatcgg
    22681 ccgaagcagg tcgacgagtt cgtcaacacc tggagcgtcg aaggttttcg agaggaaggc
    22741 acgaccacgg ccgaaatggg ctggggaacc cacgaaaaac agttacctcg cttcgcctac
    22801 gagcaccccg acggccccaa aagccaaatc tgcctggcga ggatgggcat gaacaccttc
    22861 gtcgcaagtt gggtgccgcc agaccacaac attatcggca tggtcgtccg ccacggcgag
    22921 gcgttcagca ttaccgagaa gctcaccgtg tgggaaggcg acaaggccat ttaccggcca
    22981 acggtgcact acgcctattg cccctgcgat gccgccatcg cgtctctcca tgaactccgc
    23041 ggctccgact acagactgca atcgcgaacc cgtatcatga ccgacgagat catcagcggg
    23101 gccgacatcc tgggggtact cgtgatgggg cacccgtacc agtcgtggtg gtgcggctcc
    23161 catctagaca ttgaagaggc ccgtcgcttg gtgccgcatc agaatgcgac caccatgcag
    23221 gtcgctattt ccgtcgtcgc cgcctgtatg tggatgcttg agaatccttc gaggggcttg
    23281 tgcgttcccg acaacctccc gcacgactat atcctcaata tgagcaagcc ctatttgggg
    23341 aaattcatct ccgctgcctc ggactggaat ccactgaagc actattccaa cttttttagg
    23401 ggctacaatc agccccagct cgacaggtcc gacccgtggc agtttaagaa ttttctgatc
    23461 atggaagggg attaagcccg gccatgcgac tttgagtcat caacggcaga tacggtgggg
    23521 gtgtcgcgac acatcggaaa tgaagaggga tcatggatca aaaaacctta cttgacctgg
    23581 caaacctgca tggcacgcca ttggtcgtag tggatcatgc cgtgctgcga gagaactatg
    23641 accagttccg caaacatttg ccacgcgtac aagtctacta tgcggtgaaa gcgaacagcg
    23701 atccggccat cgtgcagacg ttttatgacg tgggagccag ctttgacgtc gcgagcatgg
    23761 ctgagttctt gatcgtgcac gaaaagatca aacacctgcc ggctcagcag cgccaggatt
    23821 tcatctggga tcgtatcatc tatgccaatc ccatcaaggc catcgaaacg ctcacgcaat
    23881 tagaccagta caagccattg atcacctatg acaatcacga agaggtgctc aagatcgccc
    23941 gctacgctcc gcattcaggc ctcgtgctgc gtttgagtgt gcccaatacg ggctcgatgg
    24001 tagagttatc gagcaaattt ggcgccttgc cgggcgaggc ggtggacctc atcgcctttg
    24061 cgcacaataa taaactcgtg gtcgagggcc tgagcttcca cgtcggcagc caatgcacga
    24121 atgtgcaaaa ctacatccag gctctgcacc tcgctgccgg tatcttctcc gaggccaaga
    24181 ctcgcggatt tgacctcaag ctactcgaca tcggtggagg cttccccgcc cattacgacg
    24241 actccgtgcc cgcgtttcag agcttggcaa agaccatcaa cgccgaactg gaccgcctgt
    24301 ttccccagcc aattgaaatt ctcgccgaac caggtcgctt cctcgtcgct tcggccgcca
    24361 cctccgtcgc acaaatcatc ggcaaggccg tgcgtggagg caagctctgc tactatctcg
    24421 atgacggggt ctatcatacc tacagcggcg tgatcttcga ccactgccag tatcacttga
    24481 aaagcttcag aaaagatccc acgcagatct gtgccgtctt cggcccaaca tgtgacgcgc
    24541 ttgataccat tagtttggtc gagcagctcc ccgacatgga tcgcggcgaa ttggtgtatg
    24601 ccgagaatat gggggcctat tgcggcgcca gcagcaccta cttcaacggt ttcccgccgg
    24661 ccaaggtcgt acacgtgaac cagtgacgct ccggcaaacg aagtggagtc gatacacaac
    24721 ggcaccagtg acaagactcg tcactcatct tccaaaatca gcgctcgtcg cgaattcgca
    24781 cactcgagat tcaaggtctc aatgaatggg tggtgcattg aggcctctat gggatgaaac
    24841 gcaaccgcgc tccctgccag ggcagagtca cgttctttag gctgcttgct cgacgccttc
    24901 aagcgtatcg gcgacattga cggataggaa tgcgcggatt cacttcaaat ccgcctcctc
    24961 tccgattcgc tctcagccgc cagactctcc acgattgccc ataccaactg ttcatgcaga
    25021 cattcctgcc agaccgctca ctgagtggga aggtcttgtc aggaatcgcc atggctcaag
    25081 ttgtggaggt taaatgttcg acacgcttct ttaacatcag aaacgcctgg tcgaacgccg
    25141 catcaatctc gacctctgat ccagtcgcct tcgcaggatc cggtgtactc caatgcattt
    25201 tggtcggtgc tccgagaaat aacgggcacg cttcggccgc cgcctgatca cacactgtaa
    25261 tcacgagatc gaacgtttga tgagaaaact cattccacga tttgcttttc ggctgacccg
    25321 ggtcaatccc atgccgcttc agggtgtcga tggatttcgg gtgcacatat cctgtgggat
    25381 ggctgcctgc gcttacggca tggtagcgcc cggggcggag agcattgatc aaggcttccg
    25441 ccatgataga acggcaggag ttgcccgtgc acagcaccaa tactgtgaca attgcgcttt
    25501 ccattagtcg caacaccccg ccgttttttc agatggttca caacagttcg tcggggccat
    25561 ggtcagaggc gtgcaacagg cgccttccga cgaatcggtg gaacctgaaa acagttgagc
    25621 atcctccatc gttcgatacg tttcccacgg taggccggac ggatcctgga tccacgattt
    25681 atcagacagc gcatagcagc acaccgtttt cccttcattc acgaccggca agtggccggc
    25741 ctgaatccgg ccacgtagcg ctgcgagttc gtcctcgcca tcggcctgaa tgcccagatg
    25801 gtcgacacct ttcttggctc gcgtggagat cgcgaaattg atacgcgggt catctagcat
    25861 ccatttggcg taatctgatt tagttttgac ggggggaacc ccgaacaatg ccgtgtagaa
    25921 gcgaatggac gtgtcgagat cctctacgcc catgtggaga tgaaatcgtt tcatgatgca
    25981 gcctccctct tggcaattca gtttgtaatt ggccctgcga acaactcact gagcatgcga
    26041 gcctcagcat tccgtacaat aggggtagga gacctatgaa cagagccata tagagcaacc
    26101 actcttggag aatgtgggtg aaggttgggg gcctggctta gtcctggcca caacggcttc
    26161 aacgcttttt cgaagcaata cacacctatc aatatggtgg cggccaggat cccaaacaga
    26221 ccaagcctat ccatgtaggt ccccactttc tgtggcccta catttagcag tccggtttgc
    26281 ctgatcagag tccgagtcat gaagatgtgt tggtcgtctt tcagcatgtg attcgagtgc
    26341 agacaggaca attcgaacag cacctggaga tgttccgtca ctgatacgca tcatgctgtg
    26401 gcctccttcg aaggaaacca atgccgggta cggttacaca catgacacac tgacagcatg
    26461 acagggacct cgaccaggac gcccacgacg gttgccaagg ctgctcccga ttctgggccg
    26521 aataacgcaa tggccgtggc cacagcgagt tcaaagaagt tactcgcacc gattaacgct
    26581 cccggcgccg cgacggcata ggggactttc aaccacttca tcaacccgta ggccaaagac
    26641 gcattgaagt acacttgcag cagaatcggg atcgcaatca gcaagacatg cagtggtttg
    26701 gcgagaatat tgtccgcttg aaaggcgaag atgagaacta aggtcaccag caacgcgcca
    26761 atggtcatgg gcgcaaagct cggcaggagc gtctcctcga accacgtctt tcctcgctga
    26821 ctgataaacc agtgtcggat cacgactccc aacaccaagg gaatcacaat aaacccgatg
    26881 actgaataga caagaactgc caacggcaca gtaagcgaag aggctccact gacgagcagg
    26941 ccaacgattg gagcgaacaa caccaacata atcaaatcat tcaccgacac ctggaccagg
    27001 gtataggcag gatcaccatc cgtcagatag ctccacacaa agaccatggc tgtgcatggc
    27061 gcggcagcaa gaatgatggc gccagcaata tattgatcgg cttcagcagg actcatccag
    27121 gcggagaaca ggaaacgaaa aaagatccag gcgaagaggg ccatagagaa tggtttcacg
    27181 acccaattga caaatagggt aaccaatagg ccctgagggc gctgaccaac tttcgccaca
    27241 gcgcgaaaat ctacgttcat catcatcggg gtgatcatca gccagatcag gacggccatg
    27301 gggaaattca cgtggctatc ctggccgaac tccatccctc gcaacatctg gatcaggccg
    27361 ggagcggacg ttcccagcaa catacctgcc accatacaaa aggccaccca gacagtgaga
    27421 taacgttcga acacattgag tctcttctct gtaggaagag tttgttgagc gatgactccg
    27481 gcggcatcca taagtttcat cccgcaaaat caataattgt tgatgtatga gggcaaaaaa
    27541 atgtcacggg caacagccct gcctcgcggc gcgggatgac accgctttga gcgaattcac
    27601 cacatcctgc aactcttcaa ctgcatcgga attgagggag taatacaccc atcgaccttc
    27661 aggacgggcc aggattaacc ctgcctcctt cagcactcgc agatgaaaag aaagtcgtga
    27721 ctgtcccgtc ttaaaaatct ctgtcagatc acacacacac tgctcgccgc cctttagttg
    27781 ttcgaggatc agcagccgag tttcatccga taaggcgtga aataccgtgg ccacgtcggt
    27841 caactgtttg gtcttcgtgc gcatgtctcc atgctatatc aacaattctt gattagtcaa
    27901 tgttccatat atgccggagg catgtatgtg ccaattacca ttggctcctt cggagagttg
    27961 agcaacagtc cgaatgttac gtcctcttag ggagattggg ctatcttcga ctaacttgcc
    28021 aaaaggttcg agggtgattt ggaagcggcc cttttcgtcg ctactggctc ttttatcttc
    28081 atggctcttg gagatgcccc ccgtcttgtt gttgccactt tatttgcccg cgtggctttc
    28141 tcggaagcgt agatcctttt ctcagcttca atggtgctga acgagaattt aagagcctga
    28201 atatcttgaa cgagctgttg gacgctcttt tcaagtcgat taaatcgttg tttgatcttc
    28261 cttttcataa ccttcgcaat ttgcctcgtt ccgcgatcac ctgaatacgg caggatacgc
    28321 acaaatcaat ctgctcacgc cgatatgcaa tcccaatggc cgatccactc ataatgagca
    28381 gaataatgat cagaatgtaa aatctagact gatcctgccc ctcgttcttc actgagtaat
    28441 tcaactttca ccagacgctc ggccgcaatc caatcatcca cttcagtaac cgatcggcga
    28501 atttcataga gctcaaaggc tttgcgtgac actcgtgctc taaactcttc ttgtgtcagc
    28561 acgtgcttct gaggaggtac tgtcgaggca tcgtgcagca tagggcgttt gcttggcttt
    28621 gattttagct ttggtggagc atctcccact tgcggttctg atagttttgc gagtgcgcct
    28681 tttcgtcctg ggcgactggg tttgttgttc tgctgggaat tcatcgttct cctctttcct
    28741 cgaatgagcg tggttacatg ctgttcagtc caaccatccc ttttgcctaa agtacagata
    28801 tggcgcgccg ccggcaaaga ccatcaaaag aagcgccatc ggatagccca aaggccattt
    28861 catttccggc atatgctcaa aattcattcc gtagatgctt gccacaaggg tgggaggcag
    28921 aaagaccact gcagcaatag agaaaatttt gatgattttg ttttgctcga gattgatcat
    28981 gcctaacagc gcatccatca aaaagtttat tttgtcgaaa atgaatcctg tgtgcccatt
    29041 taacgaatcg atatcacgca acaccccttt gactcgctgg gcttggtcgg gtgaaagcac
    29101 acggcttcga agcaggaatg agagcgccct tctgttatcg atcaaatcca atcgtacttt
    29161 tgaattgaga tcctcctgcg cggcgatgcg ctcgacgtta tcgcgcatga ttcgagaggt
    29221 gatctctttt ttccggctca acacaactgt ccctacttca tccaaagccg tatagattcg
    29281 ttcaagaaca tccgcatctc gctccacccc gagttcgtaa caactcaaca agatgtctgc
    29341 ggcgtcacgc accattcccc tactcgatcg cgcctgcatc cgaaagagtc gtagtaaact
    29401 caggtcatcg tcgtggactg tcaacaatcg gccctgatgc agactgaagg acatgttcac
    29461 gattgaagcc acatcgtcag cggtttggac aaacgttgat cgaatgtgta tgccgtgctg
    29521 atcttggtaa tagcgtgcgc tcgcctcgag gtcttgtaac tcctcatcat caggcaattc
    29581 gagtgggaac acagactgca ccaactcgcg ctcctcttca ctcgggtatg ccaaatccac
    29641 ccaaaccaag tccttgcgag tcaaatcgtc tttgtcgtca atctgagcct ggatgactcg
    29701 accctcttcc aagacaaatg cgctcaacat cttcccaagc cccacttagc atcccgtgac
    29761 tagacgaggt gcctaaaaat ccaaaagaga ctccccgaca acacgataga agcgggcaat
    29821 gtaagaaccc aagccattag gagattgcgc acggtattcc actgtagtcc cgattgattc
    29881 gccgccatcg ttccagccac acctgaagag aggacttgcg tagtactcac aggcaaacca
    29941 agcatgtccg cggcaccaat tgtgcccatc gcgaccattt cggcgcatgc gccctgtccg
    30001 taggtaagat gggccttacc gattttctcg ccaactgtga ccacgatccg tctccatccg
    30061 atcatcgtac ccagccccag cgctaatgcc accgcagcct taacccatgt ggggataaat
    30121 tttgtcgctg tatctaaagt ggagcgatac tctttcaaga tctttaggtc tggcgcagac
    30181 aacggaggat catgctgctt ctccatgcga cgaagcgctt ccgtgatgag atgcatatcg
    30241 ttccgcagat tgccggtttg ttcatccggg acctgtgcca ctgatccaaa ggctgaaatc
    30301 ccttcgcgca cctctgcact aagggcccgt agagccggaa tggtctctgg catgagttga
    30361 cgagacctca agtactgtcc cacttcatgc cgtgggtcgg ttggtgctgg ctgtccatgt
    30421 gtatacctgt ccaatgtccg ttccgcttgt ctcgatgttt ctacgaaggc cggggtatag
    30481 gaaatgtcca cagtccgatt gagcgcatac acggtaggaa ccgtcccaat caaaatgagc
    30541 atgatcaatc ccatcccctt ttgcccatca tttgagccat gagcgaaact cactccggtg
    30601 cacgtaagaa tcaaaatgcc acgtatccac aaggggggag gagtcttgcc ctgaggggca
    30661 cggtagagac ttgcgattgg aatggcacgc attgccacaa gtaataagaa tgcggccacg
    30721 gaaaaaccaa acagcaggga aactaagagg gattttccaa tgttcaacgc ttgggcccag
    30781 tccactccac tcgtagcctc tgcgctcccg gccatcaatt gattcatgag ccccaccccg
    30841 atcaccgagc caatgagagt gtgagaactt gaagagggaa ggccgatata ccaggtaccc
    30901 agattccaaa taatggcagc aatcaatagc gcaaacacca tggcgaatcc ggcgcctgca
    30961 ctgacttgga ggattaactc aacagggagc aacgatacga taccaaacgc gacagcgccc
    31021 gaagccatca tgaccccgat aaaattccag agtccggacc aaatgacagc cgcatgagga
    31081 ggcagcgagt gcgtataaat gactgtcgcg actgcattgg ccgtgtcgtg aaaaccattg
    31141 acgaactcaa atcccaatgc aatgactaag gcaacaccga gcaggataaa tggccatgcg
    31201 gatgattgct tgaccgccat gagttcaaag gatagctgct caaccgtata gagagtcccc
    31261 agaaagagca ggatcacgaa ggcaaagaat ccgaacgacg caagagacct cgaaggctgg
    31321 acggtcaatg gatgtgctgc agtggtggta tgaagcgcag gtcccataga tgcaattgtc
    31381 tcccttgttg ggaaaagata cgtgagctgg cgagtatagg ggtacgctgt tacggtttta
    31441 ttaacctgcc gtatgggcac gtttaactat tgggcaaatg acgtagagac ctgacgagct
    31501 tcgcggaagg gggcgcggag tggtcgttat ctcgatccaa gatactggcc ttatgttctg
    31561 ccccgctgaa ggagcctgaa catctctttt gccaggacag gagacatgaa caatctttta
    31621 cttactattt tgaaagcaga ttgagtaacc gttctgcctc gggtcgaaat gtatgtttcc
    31681 aggcataggg ataatgcggg tgttccgcat gcaggaccga ttcaatatgt tcacgagcct
    31741 cggccaaacg gccttgcctc atgagcaacc gagccaaaat gaggtgggca ttcgtaaacc
    31801 ttccatccgc tgcaatggcc cgctttaagt acaattcggc gtctttatca cttcctccta
    31861 gcagccaggg tagctcagcg cacaagcctc ccatcatttg caacgcctgt gcgtgggtcg
    31921 gattcagctc gatggcgcgt cggatatgct gcttgagctc acctaagaca agcccacctt
    31981 tcgaaagccc tttgaggcct ccccgctacc cagcgaggcc gcatacagaa aatgagcatg
    32041 agcgctccgc tcatctatgg tgagggctcg cttcgccatt gccgatgcgg cttcgtacgc
    32101 cagctgcttt cgctcctcat cattgaacag atcatctgca atattcatat acaactctga
    32161 taactggagc aatcgatcta gagtggggcc ttctgtggag agccgggaca gctggacgga
    32221 gagaacgcgt tccaacgacg ccggatagtc cgctggaaga gtttcctcct ctgaactagc
    32281 ctgaggagga aacgtgagac agaccagcac cgctaacaac acgtatacgg ggctacgcgg
    32341 cactcgcctt gtcaaatccg atccctcagc actcgcatgg tcaacgtcga tgcagtgact
    32401 cggctccgcc cggtgaagaa aaagatgcca agccacagcg cctccagggc gagaatgatc
    32461 ccgggactcc aaatgtcacg aagtcccaac ctcatattcg gaacgctggg ggagaaggta
    32521 tccagccaaa atgtgaggac gaaagcatag agcacgccta atagcgagag gatttgatac
    32581 cacgagagcg tcccaacgcc acgaaaatcc gcccgaaggt attcggatgc tactcgccag
    32641 agttgtgtga tcgcgatagt gctcaagaag ctagcggcca tgtgcccttg taaaaatagc
    32701 cacactccgg tgatgcctgc accgactgac atgacggctg tcagtgctta aatggggatg
    32761 agtggaacag cttccagatc accctcatag gcagctttct tcgttctgcc tacgaatgcg
    32821 atgtgatact gctggaacca tgccgacagc cgtggaccac tttcctgcag aggaacccca
    32881 taacaacacc caaagctgag gcaggccagc ctccccgtcc cttcgccaaa tgcataggcg
    32941 acactcaacg ctgccaggac cgcagcggct ggaagtgctc ccttcccggt ccagttcagc
    33001 accagattaa ttccgtcgat aatcatgggc gctaagaaca agccgagcat ggaagcgccg
    33061 ccgacggtga acgtattcgc tttgccttct atgagacgag ccagaatttt tgaggctggc
    33121 acgcatagtc cgagcaagac aactgctaac agagcaatgg catcaagcgc cactgcgacg
    33181 ctttgcatta agacgattgc catcgccacg gcaaagacca ctgcagttgc ggtccaggcc
    33241 ccatagtaag tcaggttggt ccctacccag cgaccgtcag acagtttcat tcgcggcata
    33301 gaggccacaa actgccagct ctcctgtggc aacgttcgaa aggcccacca gaacagcaac
    33361 ccatacaaaa cgatcagaag cgcaatgaca gacgtactat cgcccatcga tccccctttc
    33421 taaccgccat gcgatcgtcg aacgcacctt aacatctgtc tcgatgagcg gacggccaaa
    33481 cccttctgaa aaccggctct gtaccccagg tcgaaatcga ttccgtagca ggtcttcact
    33541 gaaggagagc cggccaggct caaacaacaa tacatcggtc gagcttcctg ggcggtacaa
    33601 actctttgga cagcccttct tgagcatcat gccaatctgc atggtctgtg gatcgtcgta
    33661 ggcgacggag gagtagcatt gatcaatctg accaatcatg agggcaacca cttcgatcat
    33721 ggccacctgt cccacacgag tccctcccgg aacatccgta tcgattatgg tgacgatgcg
    33781 tctgttcttc gagtagggcg ttgctgcgac gaccacagca ccggggttac atgaatgata
    33841 gcgcccgtta atctcataac aatcaacgac gcgccctgag acgggacagt gattgtaatg
    33901 gtacttatcg ggcgtcagac ggaaaattgc ccaatcgcct cgctggaaca gccggcgcca
    33961 ttgtggccga tgtttgccga gcagttcgtc gtaatcaaaa aactttcctt taagaaagat
    34021 cgaggaatcg tcggcgaagg aaccgatcag aaggcgggca tctgcaggag aaacaatggc
    34081 cctgggatca tcatccattg gccgtacttg ccagtaccgc agccgccgct ggaacagacg
    34141 gcgcggtgtg ttgaggcgat ccgagctatc caggcattcg gacaggtcga ctgacagagc
    34201 tcgagcaaac ctggccacgg ctgcttgggt ggaagtgaac ggcctatcat agttaaagaa
    34261 tccgagggct tgggtggttc tgcgtgaagt gagcgccttc atgaaccaat ttgcattttc
    34321 ccacaaggca cggtaggccc agttgagcca ttgatcccca caaaaccgtt cagtcacaac
    34381 cagattgctg tcgcgttcga tgtactgatg cggcatactc atgaagcgag cggctgctcc
    34441 tctgttcgcc ccgcagagat ccgagcgtgg gcgatactca taatacaaag ccccaaacaa
    34501 cgctggaggg tcttggcatc ggcagtctcc gccaacaggc gcggctgctc ttctgtaaac
    34561 gcgtccaccg ccgaacgcgt gccgatcatg gcggtaacga ggccgcgata ttgcgtggcc
    34621 tgttcagaaa cgccctgatc aatggcgcac aattcctctc gtacagactc gcaggcttcg
    34681 cgcaggtatt gcgccctcaa ctctcgacgg tagtagccct ccgccgctag ggcaaactcc
    34741 tcgcccgaca gatcaaaggc gcttggactt cccacagagt tcaggatgcc gtgtgtgagt
    34801 ttcccagcag tactggagtc ctggttcgac agccgatcgc gtaaacttcg catacattcc
    34861 tgcatttgaa acgactcgat aaggtccgag gcatcctcct cgagcatagc taccagtgcc
    34921 atacggtagt cctcacaacg aactcgcagg tgccccgcat aacgccggct cgctcttgtc
    34981 ttgtgggtct tcatcagaat ccgagtgaga aatcgattgg ggcctgcctc tcgcacattc
    35041 actgtcccta ccccaaccgc cgtcgcgaac aggtattgtc ggcgttcgct ctcagtcatg
    35101 ggatcgtcgg ggatatcctc atggctcacc tcaccccttg cgatatactg aaacgccagg
    35161 gctgtcacga gagcctgcat gcttgccgct tgcgcgaagt cgtctgtgtg gttccaaaag
    35221 aggctgtagt gtcggccttc aaaacctgaa aaccccatta ctgattgagc gcgcagcttg
    35281 tacagtagat agagcgacat gcgctcgtcg aataccccca gttgaccgag gtctcgtttc
    35341 agtcgaagat cgttgcctgg gatgccgtcc agcgccgggc tttgttccgt agacaacagg
    35401 cacacgaggt agtcgattaa ccggaaatct ggcagacagt cgccacggag attaaagata
    35461 aaactgatgg ctcgatccaa ccatggtggc cctagcggtg tgatggaccg gccaaatacc
    35521 gaaagaccgg ccttcttttt ccaccgccgc cacagcatgc gcaggtgagc aaagtccagt
    35581 tcgtgcggta aaaatcccaa cgcagtttcg gggtgaaagt ctcgaaagtt catgcgatac
    35641 ggagcagcac tataggtccc tacaaacaag ggcagaaaat gttcgacgat tttgatcaca
    35701 agatcgccga ggaccttttc atgaaccgcg gtaaatcgct tgccattcgc ttgcagtgtt
    35761 tcggtcaaca tgcggctacc cagactgagg tgtgtaccgt tattggccag actaatgttg
    35821 gaagttgccg gcaacaccac gaggttgcga gtaataattc ccgcgtcttt caaccgcacg
    35881 acggcattga gttggctccg agacaatact tcatggcaca gtcccatata gcgcgatttg
    35941 tcttcgccct gatcccaacc ggacaaacag ggattcacaa acaattcccg atagaacgaa
    36001 tccgaaatga gtccattgag gcggcgctga cgcacaggcg ggtgtggcga agcatagaca
    36061 atcgcatgtt gcccggtttc atccagtccg aatgctcgat ttgcatactg aacaagtaat
    36121 tgtgtgagga gaaagcggat cgctgtttca tgcgcgatgg ctttccccat ccctcttgcc
    36181 ggatccatcc cggacacata gaaggaaaag gtctccggtg aactgttatc gttcaaaaag
    36241 tggtccaaca gtcgacgccc ttctttcgcc agcatgggaa catcggactc gatcgctcca
    36301 atgacttggg caagcgcgag ccgcagaaca tagctgaccg gcactctgac ccattcctct
    36361 ccttgttccg tgaccaaaaa acggtcaata tcagtacgag gcgttccttt cggatcgcgc
    36421 ctatttgctc gcagatcaca cgccaacagc tgattggcct ccttattaag catgtttcga
    36481 ggaaaccgaa cccaggagtt ttcccagacg gtcgattggg aatcgttgag aaactcctgc
    36541 agctgttgga tgacgatacg cggactctcc cccgtctccg ttcgatcccg aatattcttc
    36601 aaatagtttg atctgtgaat tgtttgcgcc aggtccacgt ctgctgatcg gcccaagacc
    36661 gccgcctgca actcgctttc tgatccagcc gtgatatcgc ttgaggaaaa aggcaacgtg
    36721 acagagttgg cgaggaattc caccgcctcc cgctcaatta catgacggga ttcgacggca
    36781 tagggcgatg aatttgcgga gagggcagtc gttcgactca tcaagaagat ccgagtgatc
    36841 catctctcga actgatgcct gacggagatt gtatcttagg gagtgtgtgt tacgattccg
    36901 tcatgtcgtc ggaaactttg cgtaacaccg tgctgctgtc ggcaagaccg gaaatctctc
    36961 aaggaagctg cacagtcaga tcctcaagtc gtagggcgtg aagtgaagga acgactagat
    37021 cagcctgtgc gagtcgatca gcaggaaggg tcgtggtcac tgcgacgcat ctcatgccgg
    37081 ccttgtgggc agcctccact ccatgtggcg tatcttcgat aaccagacat tcattggctg
    37141 ctatcggcga tcgtccgtgc aaggcgtgga gagcatgcag atagggggcc gggttgggct
    37201 tgccttcccg aacatccccg gccgccgtaa tgtgctcgaa catagtaagc atgctcccta
    37261 gcttcaaaca caacacaatt tcctgaagaa gtgcgcccga agcgatggcc aaccggtaac
    37321 gcatggatgc atcgctgaca aattccgaga cgcccgatac acgcggcaga gatgcacgga
    37381 atgcccgttg cacgagatcc gttttgcgac gaactaattg ttgtatatcc tccttcggga
    37441 ccacaattct atggtcattc aagattgcgc ggaaacaagc ctcatcggag agaccgacgt
    37501 aggctgcgtg gtatcgactt aacgtcaacg ggattccctc cgaccgtagc acctctcgga
    37561 atacggcaaa gtggatcgcc tctgtgtcga atagcacgcc atcgaaatcg aagatgattg
    37621 ctttgattgt cggcataggc ttgctcattc atacggatac caatccttcc atgcaatacg
    37681 cctgagcctg attgatacag agtttacagc ttccttacgg tcttgtaata cgtaggacgc
    37741 atgatggcga gcctcacatc aggcagatca gcatccttca acattggctg acttgcagta
    37801 cgagcacaaa tagccgcatc atcggtgtat caaaggagat agtctcatgc cctccgcgga
    37861 tgctccctcg ttggtgattg aacagggaac ctgttacgcc ttgttcgcct atgacattgg
    37921 attggccatc aatcttgacg aagctgagag acatgtgacg gccatcaagg aacgcggccg
    37981 catgcgccac aaagcacgag ctccacatta tttcgagtat cgtccagcgc cattgcgcct
    38041 gattcaggag accgcgtctc tcgacttcgg tgactatcga tcgagcgaag ccgtggaggt
    38101 gatgctctat gacttcggtg ccgtcactgt catttaccgc attcctatca atggaccttt
    38161 cgaaggcctc ctgagcttga gcgaaagcct gtacgaaaat gagaggctgc tcgccgactc
    38221 gcgtcaccgc ttggaccagt tggtgcgtga cattcgattg gccattgacc gtccgtcagt
    38281 ctcgcccgag gtagaagact acctggtgtt ttccgtcgag cattgcagcc catctgccat
    38341 tcaagcgttg ggcactccct ttgaaccgat gtttgcacat gtcctgcgca gcgaacgaac
    38401 gcgtctatcc gatcaggaga ttcgcgaagc gacatcttgt cgtatctcat tcagtcgaga
    38461 cgacaccgct ctcatcgatt ggaatgctgc cattgtgttc ggagaagata tggacgacgt
    38521 tcgggcagta ttggagttcg taaatgtaga gctgctggaa atgcgtacgc tagatgagca
    38581 actcgatcag gcgctggatc aggggtatgt tgcgctcaca cggaaggctc gagttacatc
    38641 ttggctacct ggttcccatg aggctgctct tacacaaatc ggacaactac aggtggacag
    38701 tgccgtgctc ttcgaacgtg tggccaatac cctgaagttg ctgggcgatc agtatctggc
    38761 cagggtctat cgcttggcct ctcagcggtt tcaccttgag gcctgggacg cgagcatcat
    38821 cagaaaacta caaacgctgg atagcattta tggaaagatg tccgaccgtg cgacgactca
    38881 acggatggaa gtgctcgagt ggattattat tgccctgatt acgatttcaa tcgctgtatc
    38941 atttctgccg atgacaagcc attaggaatg gcataccact ctaccgagaa gaggcaagaa
    39001 gctccgcaac cgagcatttt cacaaaatta gtcgagccta aacttagccc tgtcggaggt
    39061 cagttttcga agacttatcg acaccagtcg ttcgctgagg agaggcggag tggcaaaggt
    39121 caccgtgcat attttctgag acggctgaag tggtaccgta aacgcttgcc ccatagttct
    39181 attgaacagg atcatgagct cgtcatactc ggtgcgcgtc agcgccactc tctgttgatg
    39241 aatacaatgc gtggtagagg gccggacttg tgctcgcctt acccgcctgt cctttggcag
    39301 gcgatcgata atcggcctcg aggccagaat gttggccatt aatggtatga gtacatcagg
    39361 gcattgtgtg agagacgatg tagacgtggc catctaggca cgtattctgc caggcccccc
    39421 aatctataag caactctgcg gaatctcaga ttgtacgttt caacagcgct ttcaaccacc
    39481 gatgctttcc ccgagctatt cttgcagaaa ttcccagctc ccaagactat cccctttcga
    39541 cgaaacggtt gtcgtaagct tcgacggaac tgatgaaaga agcctttccg ttcttagtag
    39601 gctcgctcta cagacaaacg tctcctgtgc tggcagaacg attacctcga tcgacctcat
    39661 atgctggcac tgaatgtctt cctcgttcag gaaagtaaac attcctaacg cggctcgcag
    39721 gcgatactat gatcgagagc tgccctgctt caatctgcag cgcacccagg actgaacggt
    39781 tgataaagtc ccgcgcgtga agatgatacg gcgctgacca tgcccatcag gccggaggag
    39841 tagcgtagtg atggacccga tgaatggaga gggcgcaatc cgtccgtaaa tcctctccat
    39901 tcatcggctc ccacaaatgc tgcggaagga gattgaacga tgccctgttc ccgatgcgga
    39961 ggctatatgg caatcgaacc atcaaacgat ttttatgatc cagaaggccg gtggcgatgc
    40021 gtcaattgcg gagatcggaa ggcgttccat atccgctacc ccacgaagat ccccggcgaa
    40081 atcaccgcac gcgattcttg atcgtgggga catcacgcgc agggtccgca ttgccctttg
    40141 gatcagcacg ataccctcac catccgtcct tgcactgaca caggaggtca ccaatgaaga
    40201 tttatgactg cccccgatgc cgacatcaca gtttggtccc gactggaggc tttttgcact
    40261 gcggacagtg cgattacgcc atcacggcct ctgccttggc cgtcgagcaa cgagctcgca
    40321 acgcctcctc ccgcgcgtca aaaccctgat gcgccaatcg aaccaccgag cagaagcagg
    40381 aagctacgtc cctttactcc gcggccctgc cgacgcctcc ctcatgaacg ggctccttcc
    40441 ccgcggctga tcgccagttc catcaaatca caacaagcca agtagtggct tcgccatctg
    40501 ggcttttccc ggtatccaaa ccgattcact cttatcgaat tggatacagc gtcatctcaa
    40561 ttcaagacga cgaaaacctc tgttcgctga cacgaaccgc caacacgaag aaatttcgac
    40621 attcacacgc agccgcaagt tggtcgcgtg atggtacgcg agttgctagt catcctatcg
    40681 ttgcatcacg gatgcggcag aatgccgcga aagaatcagg aggggatcat gatctgggcg
    40741 attatcattg cagcaactta tatcgcagga atttatgtga ttcgtttggt gtggtggcag
    40801 gagtgatgac tttttccatg cagcgcaggc agagacaatg tgtaactgcg cccagcataa
    40861 cgtcgacctg tttaccgtca atgcactacg ctgagagcgc gcttcacagt aagacctatg
    40921 gccgttgaaa ctcccactgc cttctcgcat tgctgccctc tgtgtcccgg agtaatacgt
    40981 cgctctggac gccgagggtg gcgcgatttc ttatggcatt tgctgggccg ctttccctgg
    41041 cgatgtcgat cctgcagtca tcgtttctat gccgccagcc gccattgatt ctttcctcaa
    41101 cgttcgtggc aaattcctta cacaagaacc aaccggcaca tcccccgaag gtgggatgtg
    41161 aggaactcca ttgcaggcac tacaatgccc tccatgcagt cacatctttc atcaccacga
    41221 acgccacgac tccacccctc tccgtcacaa accacccttt cctttcaacg atctcgagac
    41281 cggctgggca tagtctttcc catcatgttc gccggggcca gattcattgg agaagggact
    41341 gttctcaatc tctcaccaca tggatgcctt ctagaatgtg aggggacggt gatggagggc
    41401 agctacctga cagtaggact cctcctgccg gacaacgacc atgcattggt catagacctg
    41461 gccgcagtcc gctgggtccg caggcactat ttcggcatcg aatttcttcg gctccccgct
    41521 tcagagcgtc accgtctgga tcaattctta accgcttatc gtcgttgatt ttccaacgaa
    41581 accttgctct gggccattct cacgatccgt atatatttca caacagaaca ggttacccgg
    41641 ttcccgcatc ccacgacgac tcttcacaat cccgactgan nnnnnnnnnn nnnnnnnnnn
    41701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41761 nnnnnnnnnn nnnnnnnnna gaacaggtta cccggttccc gcatcccacg acgactcttc
    41821 acaatcccga ctgagccgag acgatctgtc atctcccacc gaatgaacgc acatacatca
    41881 acacttgcca ggcttcttct tcggtcagaa ccaacggaat aaacgatgcc atatctgttc
    41941 cgggactccc atttttgagt atccagaaca attcgccgtc cgtgcgcgcc tgttgccaca
    42001 tcttgtccgt gaaatttcgt ggcagcggtc ccttaaaggt agaatagtcc aggtccagac
    42061 ccaggccctt cccatcggcc ccgtgacagg cgcgacaaaa ggcctttccc tcaaacaggg
    42121 cctttccttt ctcgcgcatt tccggcgtca ccggaaatgg attcgtcact gcacgcgccg
    42181 actcgatctg atcgaccggc acgcgaggac gcaacacctc ggaatccgcg gaccaggcta
    42241 tggcccctac gccgactacc gtccacgcat aaagaaagag ggatacggac ttcatcacag
    42301 ctattcctct ctacaaaaca acacgggcgg agaggatgcc gaacgctccc cctccgcccg
    42361 gtgcgtaaac acaacctact tactccgtcc gatgcttatg gccaaccgac tgcggatgtc
    42421 ccacttcccc gttcggcgcc ttcgccccat ccggccagag atggaaaatg atgccgtcag
    42481 tcttagccgc gaccgcagcg acttccttgg cctgcgcatc cggcatatcc aggatttgga
    42541 cgcggcccgt tgcaatctcc acttcgtgat cgtgatagta cttattccag gtttccaatg
    42601 gcacatgtgc ccgcgagacg gatttagcca cgaaatactc aatgtccgtc aacaaggcat
    42661 tcgagtccgt ggattcaaat agcaagcact gtaagatctc cggggagatg ggcttacagt
    42721 aatgatgata gggaccgtgt accgttccgt cttcaaactt atggggggcc atcacgtgaa
    42781 tggtataccc ttgagccggt gtcggcatgg ctttcgccgc tgttgcggcc ggggcggcag
    42841 cagccgtgga cttcatgtca tgagacgttt ccgcacagcc ggtcgccgtg agggcagccg
    42901 cgcataatcc gatcattgca atcgcacttc gcataatggc ctccttacca taggtcgaca
    42961 gatcgtcgat cgttcatgaa cgcgatgtta ttccgtccgc ggcttatgcc ccacagacgt
    43021 cggatgaccg accgatccat caggagcttt tgcacctttt ggccataagt ggaagataat
    43081 gccatctgtt ttggctgccg cggcagccac ttctttcgcc tgcgcatccg gcatgtccag
    43141 cacttgcacg cgaccggtgg caatttcgac ctcatgatcg tgatagaatt tattccacac
    43201 atccagcggc accgctgacc gagagacagg tttcgccacg aagtattcca catcggttaa
    43261 cacggcattc ggcgccgtgg attcgaacaa caaacactgc aagacctctg gcttgatggc
    43321 tttgcaatag tggtggaagg gaccggccac cgatccgtcc tccatcttat gcggggccat
    43381 cacatgaatg tcgaatccgt cggccggacc cggcttcata tccgcggcaa gtgctggtcc
    43441 gcccatggcg agcgcgagcg tgctcgcacc aagcacgcaa agttgtcgaa acatagaagg
    43501 cctcctctgg ttggggtgga ttgcatggat gggttttatc gacagaccga tcccttttgc
    43561 ctctctgccc gtaagagtca cagctgtccg aaaggattcg cgatttattt cggcaactgc
    43621 ggcatgctga actttatcgg ctctcccgtc aacgccttca ggaacatcac cagatcggct
    43681 ttctcctctg tggtgagacc gagcggacgc atcaacggac tcagtctggg attgctgccg
    43741 cctccctgat ctaaaaagtc gacgacttcc tccaaggtct tgaaggcgcc atcgtgcata
    43801 tacggagcag tctcggtaac gctgcggaga gtcggcgttt tgaacgctcc tttgtctttg
    43861 tctgccctgg tgacgttgta ccgtcccagg tcttccttca tcggcccgac ttgaggaacc
    43921 cccagattgt gaaactggtt gtcggtaaag ttcggcccat tgtgacacag aatacaccgg
    43981 gcctttccct taaagaggcc taaccctcga gcagcagctt catccatggc ctttacatca
    44041 ccgagcacat atttatcaaa ggcggaattc gtggagagca ctgtacgctc ataggcagca
    44101 atggcctcgg caatcccctg cagattgacc tccgtcccga agacggcgcg gaattcttgt
    44161 tgatagccct tgatcttccc caacttggca acgacgtgtt catgcgtctc cgccatttcc
    44221 acggggttgt ggatcggccc gatcgcttgt tcttccagcg accgcgctcg gccgtcccag
    44281 aattgcacat gattaaaccc cgtattgtag acggtcggcg attggcgacc accaattcca
    44341 ccaccgacgc cgatcgacgt ctgacgggag tccgcaaatc ccgttcccgg gttatgacag
    44401 aacgcgcagg agatcgcgtt gtttttggat aatcgtccgt caaaatacaa ctgttttccc
    44461 agctcaatct tagcggcata attgacgttt gaagagggaa tcggaactgc agtcggcagt
    44521 gggccgatat caggtaccgt gatgccgtcg atggttacgg tcccatgagg cgcgtgaccg
    44581 gaaggtgagg cacccaccag actctcgcca atcccgaatg tagcgacaca caacaggaca
    44641 aggatgaagc agagcggcaa aaccgttcgc accatagaga tgacctcctg tatggatttc
    44701 aggtgttgga agcgatgaac tactcatatc gcgctggcca ttgtacgcag aattagccat
    44761 tctgcaaaga gagacgccgc caagatgcgc gcggcagcgt cgatgaggaa gggaacccac
    44821 agagtatcta gcgcacggta gagcgcatga gactatcggc ttgtggagga agaaagccct
    44881 gatagccctg atgccgttcg gcgagttcca agaccgaaaa cggttgcccg tcaatcatct
    44941 cgggcgcagt aaagatctgc cgaagcggtc ctccccgggc accgacgatt tctccggcaa
    45001 agaccacgcc cttcgatttc agtttcagaa tcgcttgctc aatgtcctcg acccggacag
    45061 ctacgtgatg gaacacacga tcaccaaact tatccaccca acggggaatg atgctggtat
    45121 gtccgcgagc atccgggtac gcctggtcga caaacagtgc cggatatccg gcttttctga
    45181 agaccttggc ataccaatcg tcgtagtaaa tcgtttcatc gtatccgtag ccaagggcga
    45241 cgaattcctc cgcgcgctgg tcaatgttca tggtccgaat cgtcaaatgg tccatgaccg
    45301 gcacaaatcc aacaccgacg tcgtccagtg cgcgacgaag cattccggcc gcggtattct
    45361 gtgcaaggaa atccgccgtc atacgagcaa tcacggcatc aagctcagcg gcttgcccca
    45421 tggtttcctc cttccgatcg cccaaaggta atcccctgcg ccagctgtaa ttcagtcccc
    45481 cagttgatcg tcgtggtctg acgccgcata taggccttcc acgcgtcaga tcccgcttca
    45541 cgcccgccgc ctgtgtgctt ctctcccccg aatgccccgc cgatctccgc tccggaggta
    45601 ccgatgttga tgttggcaat accgcaatcg ctgcccgagg ccgacaggaa ggcctcgcta
    45661 tgatgaaggt gagtggtaaa cagcgcggac gataatcctt gcggcacggc attctgcata
    45721 tcgatcgcct catcgagcgt ccgataggtc atgacataca agatcggcgc aaacgtttcc
    45781 tgctgaacaa tcttccaatg attctgggct cgcacaatcg tcggctcgac gtaatacccc
    45841 ggacgagata agacgcgccc tccacagagc acctcaccac cagcttgtct cacgtcctcg
    45901 agtgccgact gataccgcgc cacggcttcg cgatcgatca gcggccccat catgacattc
    45961 ggttgaagcg gatccccgat ggttacctgc ccataggcag agacgaggcg cgacaccagc
    46021 ttgtcgtact gggaatcctg cacgatgact cgtctggtac tagtgcagcg ctgtccggca
    46081 gtgccgactg cgccgaagac aatcgctctc gtggccaatt cgaggtccgc ggtctcatcg
    46141 actatgatgg cattattgcc gctcaattcc agcagactgc ggcccaggcg acgcccaacc
    46201 acctccgcca cttgccttcc caccggcaca gaaccggtga aggagatcag cggaagccgt
    46261 tcatctcgaa ccaggcgctc agccagttca acacgatctg tcgtacagag tgcaaagaca
    46321 cccggatatc cggcgtcttc caagacgcga ttgcagagtt gctggatggc cactgcgcag
    46381 agcggcgcct tgggcgaggg tttccacaac accgtatttc ctgtcacggc tgcgatgaat
    46441 gcattccaag cccagaccgc gacgggaaaa ttgaacgccg taatcacgcc tatgacgccc
    46501 aacggatgcc attgttcgta catccggtgc tgtggccgct ccgaatgcat ggtgtacccg
    46561 tacaacatgc gcgaaagccc cacggcgaaa tccgccatgt cgatcatctc ttgcacttcg
    46621 ccatcgccct cggctttaat cttccccact tcgagggcaa tcagactgcc gagagcatcc
    46681 ttctgttctc tgagcgcctc agcaatccga cgaatgacct cgccccgctt aggagccggc
    46741 accgtccgcc aaaccttctg catgtgctgc aagccgatca ccagggcatc gtactcatca
    46801 tccgtgcaac cgtacactcg gcccagggtc tgacccgtcg agggattccg ggattccacc
    46861 actcctgccg gagtcgtacg tgtccatgtc gaggaagaac cgcccccacc cgattgttcc
    46921 cgggccaaac ccaaccgtgt aaagagttct tctgtcgtca ttattgccct aggaacggaa
    46981 acagtccccg aaccgattgt tcaccacatc gcgcaagggg aaatgttcct gccggatgaa
    47041 tcctcgatag gcggatggct gagccagaac cagatcaagc acacaacaga gggatgacgc
    47101 ggtggtcacc tgaatcgccg accaagtgcg cccgagcatc atccgagggt agactttctt
    47161 cacgtaggtc tcttcaaata attcaccctg ccgggttcct gtgaccgcgg cgtacatcaa
    47221 caccacatcc tgatgcgtct gcggaatcgc ccgctcgaga atacgcttga gggtctcacg
    47281 gtcttcattc agtttgagat cgttcatgag aaattgaatt ttttcgcaat ggccggggta
    47341 ccgcagggtc t
//
LOCUS       DAOQDS010000032        45141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_263_length_45141_cov_21.059043, whole genome shotgun sequence.
ACCESSION   DAOQDS010000032 DAOQDS010000000
VERSION     DAOQDS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 45141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45141
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_263_length_45141_cov_21.059043"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..640)
                     /locus_tag="PK589_09660"
     CDS             complement(104..640)
                     /locus_tag="PK589_09660"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNJ19908.1"
                     /translation="MNVPNSLTMLRILLIPVYVGLLNYEQFDYALAVLFIAGLTDALD
                     GIIARVADQRTRLGEVLDPLADKLMLTTGFITLSVMHLVPLWLTILVASRDLMLMLGA
                     AVAHFTHTQVDISPTVLGKGTTLIQLATLVAVVFFASRRLDLSTLDPLLYLMGGVTLT
                     SGLHYLSRGYVRITSSQA"
     gene            817..1137
                     /locus_tag="PK589_09665"
     CDS             817..1137
                     /locus_tag="PK589_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19909.1"
                     /translation="MSGLPLLFKKEGLIERHQVEGIDPSDRYFNRAVLVNRVAAGYTG
                     KMTYEAYAVEGTPHSTTSAAVKAVVDKLQSVGFTRMRTRLNFKGNRYLAEKETWTDYP
                     DLSA"
     gene            complement(1134..2258)
                     /locus_tag="PK589_09670"
     CDS             complement(1134..2258)
                     /locus_tag="PK589_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNJ19910.1"
                     /translation="MNDPIQSALQAAVDDGTFPGAVLAVRLRGEIVFESAVGRLSRQG
                     SEEAVTDHTCYDLASLTKVLATTTALALLIQRGRLKVDDRIDGILNELRDSAIGAATV
                     RQLLTHSSGLPGWRPLYERIAAIEATQPGFMGSPAACEAVTGYIAKEELIYERGSRSL
                     YSDLGFMLLGFAVERLSGEPLDQFCSGQIYEPLGARPLAYARRGPLSALFIHYDAECI
                     IAPTEEDPWRKRMLCGEVHDENAYALGGVAGHAGLFGTARAVLAVSQAWMDGWHKKRG
                     LLEHEIVRTFTTNRQGVPNSSWALGWDTPSVRSSSGTCFSLESFGHLGFTGTSLWIDP
                     VKELEVVLLSNRVHPTRRNEQIRVFRPHIHDVICRELLRN"
     gene            complement(2377..2889)
                     /locus_tag="PK589_09675"
     CDS             complement(2377..2889)
                     /locus_tag="PK589_09675"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HNJ19911.1"
                     /translation="MKITPIDIQQMVFQVKFRGFDRDEVNRFLEELALTVENLNRENS
                     LLREKLTTTEQQVADLRRTEATLSNTLVSAQTLAEDVKRSAQREADLIVKEAELKASE
                     IIRQARGSLTEMQRGVADLQKQRLMMVERFRSTLRSFERMLEVEESDAYQAEGASVEG
                     KLAGESSPAR"
     gene            complement(2911..3207)
                     /locus_tag="PK589_09680"
     CDS             complement(2911..3207)
                     /locus_tag="PK589_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNJ19912.1"
                     /translation="MFVVSNVLQGTATVLDTVLWLYMWVIIARALISWVNPDPWNPIV
                     QFLERATEPVLTPIRRLIGWRMGMDLSPMIAILILVFLQYAVVQSLRDIAVRMH"
     gene            complement(3207..4013)
                     /gene="proC"
                     /locus_tag="PK589_09685"
     CDS             complement(3207..4013)
                     /gene="proC"
                     /locus_tag="PK589_09685"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNJ19913.1"
                     /translation="MITRNIAVLGGGQMAEALIGGLLASKVSEPQFICATDPVSARRD
                     LLKSRFGIQVGEDNAKAVAGADLVLLAVKPQVLPAVLQDVSSALRGKLVVSIAAGVTT
                     AWIQERVSAARGIVRAMPNTPALVREGVTALAYAADLAADDVAAVRALFEAVGSVVSV
                     EERLMDAVTGLSGSGPAYVFVAIEALADGGVKMGLPRATAQLLAAQTVLGAARMVLER
                     GQHPAQLKDQVASPGGTTIAGLHQLEVGGMRGCLMAAVEAATKRSQELGR"
     gene            complement(4000..4725)
                     /locus_tag="PK589_09690"
     CDS             complement(4000..4725)
                     /locus_tag="PK589_09690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNJ19914.1"
                     /translation="MDSELGTIAGRVRAVFDEIHRATVRAGRAPDSVRLLAASKTVTV
                     DRLREAVDAGIRHLGENRLQEALPKIETLDREGVVWHFIGTLQRRKVKSVVGRFETIH
                     SVDSLALAEEIDRQAKAAGLKQRVLLEVNLGGESSKGGFAPAAVAAALPALNQLEQLD
                     IRGLMAIPPPMPTAEDARPYFRQLRELAQALTAQGCRNINMQELSMGMSHDYAVAIEE
                     GATYVRVGTAIFGARSEPYHDHT"
     gene            complement(4820..5557)
                     /gene="pgeF"
                     /locus_tag="PK589_09695"
     CDS             complement(4820..5557)
                     /gene="pgeF"
                     /locus_tag="PK589_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNJ19915.1"
                     /translation="MASEFITLPSFATHADGAEHFFGTRLSAIPVTPGRASAHRTEQQ
                     DHRAPVILSVKQVHGTDALVVDRPVQQGELFEGGWDALVTNQPGLMVTVRTADCVPVL
                     LHDPVRQVVAAIHAGWRGAVAGIVPKTVALLASRFGTVPKDVRMAIGPSAGACCYEVD
                     EPVLTRLRDVFPEWQSVVSPVNPQKAHLNLRAFVRRQAMHEGLEGERIATVEACTICQ
                     PDEFFSYRREGVVKATMVSGIALVSSR"
     gene            complement(5598..6800)
                     /gene="ftsZ"
                     /locus_tag="PK589_09700"
     CDS             complement(5598..6800)
                     /gene="ftsZ"
                     /locus_tag="PK589_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNJ19916.1"
                     /translation="MFSFQEEPQSPVRIKVIGVGGAGCNAVNTMITGGLCRVDFVAAN
                     TDVQALERSQASYKIQIGPERTRGLGAGAKPEVGRDAALESKDEIRESLVGADMVFVT
                     AGMGGGTGTGAAPIVASIARELGILTVAVVTKPFQYEGHRRMSHAEEGIRDLGRHVDT
                     LLIIPNQRLLGIVDKATPLLDAFKVADDVLRQAIQGIADVITTTGLVNVDFADVRTIM
                     AHTGRAVMGMGIGRGANRAQEAAQKAICSPLLEEGSVEGARGVLLNITGGPNMSLHEV
                     EEAASIVQHAADAEANIIVGQVINPEIGDDLIVTVIATGFEREEQPAARPAAAAERPA
                     ARTPNGRPAQQVLTGVHAAGSDRPIKDIDRPTFLRRMGETREAVERIAVVGDDEWDVP
                     TFLRKQAD"
     gene            complement(6853..8088)
                     /gene="ftsA"
                     /locus_tag="PK589_09705"
     CDS             complement(6853..8088)
                     /gene="ftsA"
                     /locus_tag="PK589_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNJ19917.1"
                     /translation="MSKRDHILVGLDIGTTKICAIVAEVPEEGPLNIIGVGSCPSRGL
                     RKGVVVNIESTVESIKKAVEEAELMAAVQINSVYTGIAGSHISGENLKGVVALKKQEV
                     TRDDISRAVESARTLAVIPHERRILHVLPREFMVDDQEGVREPLGMSGNRLEVNVHVI
                     TGAVTSAQNIIKSVNRAGLDVVDIILQPLASSEAVLSAEERELGVAMVDLGGGTTDLA
                     IFLDGSIRHTAVLPIGGQNLTKDLAIGLLTSQTDAEKIKVQHGIARTELVHGHQMVEV
                     PSVGDRPPRQFTRRDIAEILEPRVEEMFDLVKREIVRAGYEGMLGAGVVITGGTSLLE
                     GMPDAAERGLNLPARRGAPTGIGGLRDIVSNPMHATGVGLLLHARQHADNLEAAGIRH
                     GKGLGKVFDRMRSWMFEFF"
     gene            complement(8094..8963)
                     /locus_tag="PK589_09710"
     CDS             complement(8094..8963)
                     /locus_tag="PK589_09710"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNJ19918.1"
                     /translation="MPLRWRNTRPAKLNPRANARSESGLERRRGGATGGYWTRLGRGL
                     LITLRVIATVTVLVGGCSGLFVLAREIGPLTREWFLVRSVSVNGLHHVTRKEVIGRLA
                     LKPDTALYSINPTWLAERIKTHPWIKDATVVLKPLHEIHIDIVEREPAVVVRTLAENL
                     LTDAEGVLLARLGSSDDPTLPILSGVDGKRLAQGRAEDRRPVQVGAALARMVGQTTGG
                     RPDINVGNLNNLVVEVQGVTFQFSESSMNQQWSRFLKMRPALRDVAFDGEGARANEID
                     LRFADRVIVRGRG"
     gene            complement(8966..9964)
                     /locus_tag="PK589_09715"
     CDS             complement(8966..9964)
                     /locus_tag="PK589_09715"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNJ19919.1"
                     /translation="MKGVHVTERKPLTRSKIGVLMGGQSSEREVSLKTGEAVYRSLVR
                     SGYDAVAIDVGPGLSQTLQDQAIEVAFLALHGPGGEDGAIQGFLETLGIPYTGSGVRA
                     SAIGMHKVVTKTELAAQGIPVPRGTVVWRGTAPTLNRILTSCKLKLPIVVKPASQGST
                     IGVTIVRQPSQWKEALKVAHRYDPEAMVEAFIPGHEVTLSLLGTADGSVAGLPAVEIV
                     APDGFYDFSAKYEKGRTQYLCPAPLPAAVSRDIKQLAIRTYQALGCNGAARVDFRITA
                     KGKPYVLEINTVPGMTETSLLPMAAGKAGLSYDALTEQILQSALRRAGTGSFRAVR"
     gene            complement(9974..10906)
                     /gene="murB"
                     /locus_tag="PK589_09720"
     CDS             complement(9974..10906)
                     /gene="murB"
                     /locus_tag="PK589_09720"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNJ19920.1"
                     /translation="MSRVARKEQSARTRKDWARILEGIRGTITYEASLQSYTSFRIGG
                     PAEVLVEPADVDDLCRVVAQAHAERLPIFVVGGTNLLVRDGGIRGLVLSLRQLKAIRQ
                     EAGSVLYAEGGVGMPTLIGYAIRRSLAGLEWGAGIPGTVAGCVVMNAGTRLGEMKDSV
                     KAVRMVDPKGRVMDIPAAEIPFSYRKAHLPRGIVAAVWLQLKPGAHDQIEKTVKEYLQ
                     YRKNTQPLTLPSAGCVFKNPPQDSAGRLVDVAGLKGARVGDAQVSEKHANFMVNLGHA
                     RAADVLALIRKVRAGVKKASGVTLELELKVVGQA"
     gene            complement(10928..12424)
                     /gene="murC"
                     /locus_tag="PK589_09725"
     CDS             complement(10928..12424)
                     /gene="murC"
                     /locus_tag="PK589_09725"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNJ19921.1"
                     /translation="MRPATALAPYEQRERERQQQDRNSGQRSFRGQELRRTAMFRKTQ
                     HIHLVGIGGSGMSGIAEVLLTLGYKVSGSDLSQSETTRRLEELGGRIAIGHHEANIGE
                     AQVVVISSAVAATNPEVVAAKARQIPVIPRAEMLAELMRLKFGVAIAGAHGKTTTTSM
                     VANVLAQGGLDPTMVIGGKVNALGSHARLGRGDLLIAEADESDGSFLRLAPTIAAVTN
                     IDREHLDHYGSMERLNDSFLEFVNKVPFYGLAVLCSDDERLRNLLPRVVKRFQTYGLN
                     EYPDHVPDFRATDIALRQWGSEFRVFFRGRNLGPFRLAIPGIHNVSNSLVAIAIGMEL
                     DIPVDLIRKGLAAFSGVERRFHLRGEKAGIMVVDDYGHHPTEVRATIAAAKQGWDRRV
                     VVLFQPHRYSRSRDLMQEFSHAFDQADALFMTEIYAAGEQPIPGVSGAKLVEAVKAAG
                     HPSATFVERKETLAEQVLPTLKSGDLVITLGAGDIWKAGLGILDRLPA"
     gene            complement(12393..13505)
                     /gene="murG"
                     /locus_tag="PK589_09730"
     CDS             complement(12393..13505)
                     /gene="murG"
                     /locus_tag="PK589_09730"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNJ19922.1"
                     /translation="MIAAGGTGGHLYPAIAVAREFLRRDPSTRILFVGTTRGIEQKVL
                     AHEGLPLRCITAHPFMGKSPVEMVKALITLPVSLWQSLGVLKQQSADLVFGVGGYTSP
                     AMLVAAFLSRVPGVILEPNAYPGLANKAVAPLVQRIFLAFESTKQFFDRRKTSVVGVP
                     VRQAFLDSSSPETARSETGPKRHLLIFGGSQGAKAINSAVIDALPMLATMNTRLTITH
                     QTGEADHARVAAAYQQAGISAQVVPFLYDMPTALRAADLVVARAGAMTIAELTVCGKP
                     AILIPLPTAIYNHQLRNAEVMAKAGGAVLLPQAELTGAALAHAMTEIFSDQQRLETMS
                     RHSWNMRRSDAAETIVRECYDVIRRRHEASDSARAL"
     gene            complement(13511..14728)
                     /gene="ftsW"
                     /locus_tag="PK589_09735"
     CDS             complement(13511..14728)
                     /gene="ftsW"
                     /locus_tag="PK589_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNJ19923.1"
                     /translation="MAQHALGTLMLPWSTSTQRASKRVPVDPALLAVTLILALIGVVM
                     VFSASAVVAGNRFHDPWYFLKRQVAWLGVGLLVMHLISRIDYTIWKKLAIPLLCGATL
                     LLVLVLIPSLGNVAKGARRWLHLGPINIQPAEVAKFVAVIYAAAYLTKKQDQITQFAR
                     GLLPPLIVIGLLSGLVLLEPDLGTVVVMGLVVVTLLFLAGARIKHLAILSLCALTGVA
                     GLILTSPYRWKRFLMFLDPTKDPSGAGFQITQSFLAFGSGGPFGVGLGEGKQKLFFLP
                     EAHTDFVLALVGEELGLLGTVTIVLLFGLFVIKGFQIAGRARNAFGKHLAMGITMLIG
                     MQALVNAGVVTGLLPTKGLTLPFVSYGGSSLVANLFGVGILLNISRDRQGGHGGQEGG
                     GSRGARKRGVITE"
     gene            complement(14764..16152)
                     /gene="murD"
                     /locus_tag="PK589_09740"
     CDS             complement(14764..16152)
                     /gene="murD"
                     /locus_tag="PK589_09740"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNJ19924.1"
                     /translation="MDVKGLQVTVVGLARSGVGAARLLNHLGARVTVADRKEPQELAT
                     ILSQLDQSGIAVKVGAQYESALEGADLVVISPGVPTQLDALNRVRARGVRVIGELELA
                     SRYMTAPIVAVTGTNGKSTTVTLIGKFLKESGKRAFVGGNLGIAASEAALTCVQAKPG
                     SPPPYEYVVLETSSFQLETIEQFHPFIASILNVTLDHMDRYNSVEDYVAAKARIFTNQ
                     TAGDYSLFNLDDTRVAALRGNTKAAVLGFSRSGATVSGVAGATVLDGDLIVTTVRGQR
                     EEICRRGDMRLIGLHNVENVMASVTYGLLCGCSIEAIRAVLRFFPGLEHALEVVRERR
                     GVRYVNDSKGTNVDAVLKALEGIEQPIWLIAGGRDKGGDFSRLEGAIRERVKGLILIG
                     EAAARIQAAMGDFDRCRPAATLRDAVELAAREAQPGEVVLLSPACASFDMFADYQDRG
                     RQFKALVQALPA"
     gene            complement(16163..17239)
                     /gene="mraY"
                     /locus_tag="PK589_09745"
     CDS             complement(16163..17239)
                     /gene="mraY"
                     /locus_tag="PK589_09745"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNJ19925.1"
                     /translation="MLYNWLYPLHTQFSFLNVFRYQSFRIIYAAVTAFLIAFVMAPWV
                     IRKLQEIKLGQQIRDDGPKRHLAKSGTPTMGGILIIFAVVLSTLLWADMTNRYVWLVV
                     VATVGFGAVGFADDYLKFIKRQSKGLSAAQKFSGQFLVALAIGVFLYTLPSYTTKLSV
                     PFFKFFTPDLGWFYIVFVILVIVGSSNAVNLTDGLDGLAIGPVMIASLAYTIVAYVTG
                     NRVMAEYLLIPYIEGAGEIAIFTGAILGSSLGFLWFNTYPASVFMGDVGSLPLGAALG
                     TVAVISKHELLLLLVGGVFVIEALSVILQVGSYKLRGKRIFNMAPIHHHFEMKGWDEP
                     KVVVRLWIIAILLALLSLSTLKLR"
     gene            complement(17297..18733)
                     /gene="murF"
                     /locus_tag="PK589_09750"
     CDS             complement(17297..18733)
                     /gene="murF"
                     /locus_tag="PK589_09750"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNJ19926.1"
                     /translation="MALFTVEEICEVLSAKSPTGLSPQDLKQRIRRVVTDSRLVRKGD
                     LFIAFQGERFDAHTFVPKVFAQGAVCAIVQEDYRLPPMKPRTGAPIVLGVRDTLEAFQ
                     RLATHYRNRFAIPVIAITGSNGKTTTKEMVAHVVAQRWKTLKTEGNLNNRIGVPQTLF
                     QLAPRHQAAVIEMGVDQQGQTTRLCEIARPTVGVITNIGPDHLEFFGSMEGSAQAKAE
                     LLDHLPQDGSVVLNADDEYFDYLAARAQCRVVAFGSSSKAAVRAANVRTDDKGGTLFG
                     LVLPGKTRQTEVRIRTQGQHNVSNALAAAAVGHALGLSGAAIAEGLAKFRPAAMRSQI
                     SSSHGVQVINDCYNANPASMKAAIQLLAQLGRDKRSIAALGDMLELGADTKRMHRDVG
                     AFLAEQGIGHLMACGVLGRELAAGARQAGMPADRITEHPDAQAAATALARMVRQGDVV
                     LVKASRGMRMEQVVDALTGMRRVAKKAC"
     gene            complement(18779..20281)
                     /locus_tag="PK589_09755"
     CDS             complement(18779..20281)
                     /locus_tag="PK589_09755"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNJ19927.1"
                     /translation="MTLDELISPIQGRLGVLERSGDQRVAIDALTDDSRHVQSGALFV
                     AVQGERVDGHEFLDRVVKAGAAAVVVGRHVQVGSTPCIRVQDSRAALGMVGSRFYGDP
                     SAALRMVGVTGTNGKTTTTYVVKTMLEAANRQVGLIGTVAYLVGKESIPASHTTPGAL
                     ELQKLFARMVEKRLDTVVMEVSSHALALDRTAGSEFDVAVFTNLTQDHLDFHLDMERY
                     FQAKRKLFAELGQPDSRKTKKRAIINADDPWGARMREACTVPVWTYGLHQQADIHADE
                     VTLSPAGTSFTLRTPAGTCPIQSRLVGEHNVSNLLAAIGVVLHEGLTLEQVRAAVGAV
                     SNVPGRFERVDAGQDFTVVVDYAHTEDALVRLLTAAQALRTGRIITLFGCGGDRDRTK
                     RPKMGRAAVQYSDVVILTSDNPRTEDPASILSEVEVGVKDALTARSHVRYRMIADRRA
                     AIEAAINEAKAGDMVLIAGKGHEDYQIVGTTKHHFDDREVAREMIQARRS"
     gene            complement(20283..22001)
                     /locus_tag="PK589_09760"
     CDS             complement(20283..22001)
                     /locus_tag="PK589_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNJ19928.1"
                     /translation="MASPSFRGRRILVACGLSGAFILVIVRLVNLQVMQSAELTVKAD
                     RQHQKNVTLEGARGTIYDRNSKVLAMNMDVPSVFGVPASLGNPGVAARNLSPILHVKA
                     TELEKKLKQEKHFVWLARKLEPEQGRRLERLGLDGVGVVMEGRRFYPKGPLLSHVLGF
                     AGMDDRGLEGVELRYEQYLRGEKRAVVLQRDALGRAVFPKGLNEEGAAAGHSLTLTVD
                     EVVQYIAEKELDEAVTRSSAKSGTMIVMDPRSGAVLAMAVSPRFDPNTVGALAPDRWR
                     NRALTDTYEPGSTMKAVIAAAALEEKVMTPGSMIYGENGQFAIANTIIHDHEKAGWMT
                     FAQMIQKSSNIGAAKVGMALGEWRVFDYLKEFGFGDKSGIDLPGETAGLLRGPRQWGK
                     RSLASISMGQEVGVTPLQMVTAISAIANGGVLMKPYVVSEIRNGKGQLVAQNMPQAKR
                     RIISADTARTLTTLLEGVVTHGTGTKAAIPGFRVAGKTGTAQKVDPRTGKYSSTLLVG
                     SFLGYVPAEDPRLAMIVVIDEPRGEGWGGVVAAPVFRRVGEQVLNYLGVAVDEPVKLA
                     MAGLES"
     gene            complement(22001..22333)
                     /locus_tag="PK589_09765"
     CDS             complement(22001..22333)
                     /locus_tag="PK589_09765"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNJ19929.1"
                     /translation="MKAVAFAAVTSLVLLFVWERVDIVRIGYHIERLKAQKVLLERER
                     DELQVKLSGLTAPERIARLASDKLGMLQPEKGQVVVINFEPETPANPVVAEGEIRIAK
                     NMVTRRAR"
     gene            complement(22330..23253)
                     /gene="rsmH"
                     /locus_tag="PK589_09770"
     CDS             complement(22330..23253)
                     /gene="rsmH"
                     /locus_tag="PK589_09770"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNJ19930.1"
                     /translation="MDSARGSHEPVLVDEIVFWLQCKPGGVYVDCTLGYAGLATRILH
                     HTAPDGILIGIDRDADALEESRARLQGVMPRVHLRHGNFSQLKTLVAQVGVQHVDGVI
                     FDLGVSSPQLDRPERGFSFRDDGPLDMRMDRSEGRTAADLIREMPERELADVIYQLGE
                     ERYSRRIARAIVEARTQRVIGTTRELAAVVEHAVPASYRHGRIHCATRTFQALRIAVN
                     RELEVIEPALRDAVDLVAPGGRVCAVSFHSLEDRIVKHTFRALANGPDAAVRVLTKKP
                     VIASESERDHNPRSRSAKLRVVERMSKESMQ"
     gene            complement(23564..24856)
                     /gene="asnS"
                     /locus_tag="PK589_09775"
     CDS             complement(23564..24856)
                     /gene="asnS"
                     /locus_tag="PK589_09775"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNJ19931.1"
                     /translation="MPVIYIDEAATHAGQEVTIRGWLRSRRDKGKLHFLIVRDGTGDI
                     QAVVSKGAVGDEQFAHSAELTQESSVVLTGTLRQDARAPGGYELDVKRLAVLQVAEPF
                     PIQPKEHGTGFLMEHRHLWLRSSRQHAVLRIRHEIIRACRNFFDDRGFTLVDAPIFTP
                     NACEGTTTLFQTQYFDETAYLTQSGQLYSEATAAAFGKVYCFGPTFRAEKSKTRRHLM
                     EFWMVEPEVAFAELPDMMDLAEQFLAHIVAAVLKTRRAELQILERDIAKLERVTAPFP
                     RITYEEAVDILQRKGNPAKRGDDFGADDETILSKEFDRPVIVHRYPAALKAFYMETDP
                     ARPDLALCMDVLAPEGYGEIIGGGQRIHQHEKLLARIREHHLPVEAFQWYLDLRRYGS
                     VPHAGFGMGIERAVAWICGLEHVRETIPFPRMLYRLYP"
     gene            24992..26086
                     /locus_tag="PK589_09780"
     CDS             24992..26086
                     /locus_tag="PK589_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19932.1"
                     /translation="MIQTSTLHPRHWLASALIGLLLVGLLPAVQAGEFKSDNPLNQPG
                     KIYWCPNRAADKRITATPDAGCTALYDAERDEKKPAALNQDERNDAATKEPLKILDIQ
                     NEASKFTRDYREFIDCCASEWDSLDRIDELLEQSNHILMSVQQKGIYNSAGFGVGSGA
                     SGLGGGPGQSPKLGTFARQWTLSEIVGTVAIARDDLKKLRTRMKGLITRYEKLDSLDY
                     EQRGREERRIEDEREAITHDLRPKRPATSAPTGMDIQDTTIPTRIGGDIENTHLNRSL
                     NPSFGADIGTTVSPYSNVNESLRPRRGEDLRDSVLTDRVGTQTQDTTLPNASGFEINA
                     AENAEQSSSAHIRGVGPSIGDSSLNSQSRR"
     gene            complement(26127..27149)
                     /locus_tag="PK589_09785"
     CDS             complement(26127..27149)
                     /locus_tag="PK589_09785"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19933.1"
                     /translation="MTKFLASMFLLAGLLLGGCGGSDPVVVIQLHPTNPDILYIATND
                     YIYKSRDGGKTWSNLSKGMSYSRVIAMAVDPVYPATIYAGTKGDAVYKSYDGGQRWTS
                     VRTGLDDATISSVVNQFVFDPSDDNHIFLATTMGVFESKNAGERWVKRMEGMKEVLMV
                     VTLGMDPAHPSVMYAGTSGGVYKTNDQGAHWLQVNNGLVPEGMVKTSRALGVTSVQVD
                     PFAPDTVYAATLAGLYKTTDGAVSWVRIGESLQDQMIVAMVLDHAQAGVMYLAGRDGI
                     HRSGDGGKTWIVLNEGLTTTNIRSLAQSAMDPHVLFAGTNGSGLYKSVDGGAHWQAMP
                     SVFPKD"
     gene            complement(27296..27793)
                     /locus_tag="PK589_09790"
     CDS             complement(27296..27793)
                     /locus_tag="PK589_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNJ19934.1"
                     /translation="MADQSSFDVVSEVNMQEMKNVVDQATKEIKQRFDFKDSKTELTL
                     KEKEKELVVLSDDEYKLNAVIEIIKSKCVKRGVSLKAFEYGKIEEALGATVRQVIKIQ
                     SGISSEKAKEITKAVKESKLKVQAQIQGEQVRVISKSKDDLQSAIAFLKGKDFGIDLQ
                     FTNYR"
     gene            complement(27821..29827)
                     /locus_tag="PK589_09795"
     CDS             complement(27821..29827)
                     /locus_tag="PK589_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNJ19935.1"
                     /translation="MKTALIVLWMLLSILGAVALAHVVGVINPTEKVNGLWLVVAAAC
                     IYVLAYRFYGRWLARKVVQLDDRRLTPAVRLNDGVNFHPTNRVVLFGHHFAAIAGAGP
                     LLGPVLAAQFGFLPGFLWLVIGAVLAGAVQDFIILVASMRRNGRSLPEIAHDELGSIT
                     GTATAVAVLFIVVVALAGLGFAVVNALYHNAWGTFTIAMTIPIGFIMGFYLQKFRPGA
                     VAEVSVLGLVLLVTAVLFGRVVGQSPYAWLFEFEKPALVWLLAGYGFVASVLPGWMLL
                     VPRGYLSTFMKLGVVFLLGLGVILMAPTIEMPRLTAFANGGGPIIPGTLFPFLFITIA
                     CGAVSGFHSLVSSGTTPKMIEQESQAVVGYAAMLLESFVGVMALIAASVLIPGDYLAI
                     NTMLSAETLTAMGFAPSRVAELSQLVEVNVAGRPGGAVSLAVGMASIFSALPGMAGLM
                     AYWYQFALVFEALFILTTIDTGTRVARYLIQEMAGRVYAPFRQMNWWPGVLLSSAFVV
                     GSWAYLIGTGSISTIWPMFGAANQLLGTLALCIATTVLIKMWKSPYLWVTAIPMLFVG
                     GITLAGSYEMFWMFLAKAATLASGQAFALYLDAVLVAVVAILGVVVLSDSMKQWYGYV
                     ILKRPFSSSEVIATAGGGSAGRAQTAIHRHDDRKFTLPHGGGCC"
     gene            29969..30364
                     /locus_tag="PK589_09800"
     CDS             29969..30364
                     /locus_tag="PK589_09800"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ19936.1"
                     /translation="MSPATPSEPSGRERREFYRITVLLPMCLQMAGDETEGELIETSV
                     NLSAGGIGVMVTTPYKSGDVLSLTLLLPDQVRFKASIDVLRLDPLPLLGGVYRLHARF
                     VGLPAHDRELLVRYILRFQRDHLQEHYSA"
     gene            30469..31476
                     /locus_tag="PK589_09805"
     CDS             30469..31476
                     /locus_tag="PK589_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ19937.1"
                     /translation="MRMLTWLLGTIVALATIGAVYQIAGMVLDRQHYPPPGQLVDIGG
                     HRLHLFCLGTGSPTVLLEAPAPGWSLYWSTVQPQLARTTRVCAYDRAGLGWSERGPLP
                     RTGQRMAKELHRLLERAGIRGPYILVGHSLGGLIARLYQHDYPQEVVGMVLVDAGHES
                     EMRQAEFRLFANAGKSMLPVIRALTMVGITRLMASYDQLPPLLMRQEEKVSAETRPML
                     RAGWLRTGYYSTLTDESDGLIETLEQVRRSGSLGNLPLIVITATGPVWWPDMPGQVNQ
                     AKFRKMWLELQQGLTTLSTNSRQVFADQSSHFIPFDQPELLTDVVRQLIDRQRSAPYR
                     P"
     gene            complement(32230..32667)
                     /locus_tag="PK589_09810"
     CDS             complement(32230..32667)
                     /locus_tag="PK589_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNJ19938.1"
                     /translation="MTSYHAALVAPIGQDSFDRQIDRLLEDAVSAFGVSGIAWAPASN
                     AWEDDNGFYVQVALPGWEPQDVSLEVAKHVLSIKGERKEQPQPGKTVHVQEIGDGRFM
                     RLFRLPTYIDSEKASAVQKNGLLTVTFPKREEAKSRRIMIEGT"
     gene            complement(32915..34483)
                     /locus_tag="PK589_09815"
     CDS             complement(32915..34483)
                     /locus_tag="PK589_09815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNJ19939.1"
                     /translation="MRLTDPSPIGQRNEALWGTDLVVAACLFLMVFGSGVVHIQSFPP
                     LFFDEGWTVCVARTWVESGHYGCLLRGEPAPPSLAAHFPVVASVAASFALFGVGVWQT
                     RVVGLLYTFAALLLLYAVARRLFGRSVAIAALALLILVPLKWSIHPLYLGRQVLGEMP
                     LLCFLLAAFVCFLRSAHRPLWLVAAIGCWSLAFMTKAQVAPFLIASFVGTIVILTLRR
                     NWSAVRQLALVLIGSWAGFRFLIEAKNWLLSGHTMAHPSTDGMTEAIALVFVPAIRLE
                     TIQYLFVAWAEYPLGLAYAAWRLHRTPASRDEESVAQTAQIMLLLLAGSWLAWFAFLS
                     AGEPRYALPGLFLSAPFAALFVCEATRGFDVRYLLRTLAELVPGRRVTPQGFKAAVVV
                     LLLAIMGWGAVQERYAFRAREDDRDLFAVTQHLNTTTSPTALIETYDSELFLFLNRPY
                     TYALPKHLVEIIRREQGQAQAVTYDPLQQNPDYLVVGEYGRWAGFYKPLIAQNRVRLV
                     TTIGRYQIYEPVRS"
     assembly_gap    34708..34807
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<34808..35700)
                     /locus_tag="PK589_09820"
     CDS             complement(<34808..35700)
                     /locus_tag="PK589_09820"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNJ19940.1"
                     /translation="MSDVQLRTKAWFGDEPLSINFPPAWNIVEVGPQDAPALSATAMR
                     EKLNVPIGSPPLREIAKGKTKCVIIVDDLTRPTPAADLLPSIIAEIQAAGLSEHAITI
                     LLAGGTHPPASAQEIEKKVGANLSPAVRVMAHDSRGALVDLGMSPGGLPIRINRTVME
                     CDLKIGVGCIYPHPIAGFSGGAKIVLPAVCGVETTRMMHDYLRGSRTRGGSIHTELRQ
                     DMVAVARKAGLDFIANVTLNHRREISGLFAGDVVKAHEEGVRAAQRMYAVPVVSEADI
                     VVADMYPFDTSWQFAQDRGMWP"
     gene            complement(35697..>36182)
                     /locus_tag="PK589_09825"
     CDS             complement(35697..>36182)
                     /locus_tag="PK589_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19941.1"
                     /translation="EDGYVFNPLFTGAGEDWELIWRLRKRGAKMVFVPNPVTHLRRAT
                     GLQHLRHSFHRGAGIAILFRVMRRDSSGVVPQDSLLWGEGGKKTKPRWLKAIWVKMLG
                     PFEWQKFQRKRHFCLFWLGEKCQAAGFFWEMCRKRSMTHIDRKTKPMVQAVSSRVESK
                     S"
     assembly_gap    36183..36282
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(36482..38026)
                     /locus_tag="PK589_09830"
     CDS             complement(36482..38026)
                     /locus_tag="PK589_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNJ19942.1"
                     /translation="MSAKAVSKGILSVGGWSMAKLATSAVVLPILARFLGIEGYGQYA
                     YYLAVLLLASQFANVGMMQTMTRRIAERPDDAAWCRGVARSGALINGTGVVFVGLAAG
                     VLIWGTASHETARVPIALAVVGVLVFDQVWFYARGVLHGLRHEERAAVPGIMGVLMAG
                     SFGIIAAVTGMGVVGVLVGLLVADIFVAGACLRAVMVALNGLGLPASQGPPALPTGAV
                     LRFGLSAMIFSVLNMALCSLDIILVRHLAGEAQAGLYAAAVQWSQFVWFIPIAVEGVM
                     LQATARLWAEQRVEAVTSLVSRLVRYVMLGTVFLLIVVFVLGDRIITVYFGSQFSEAA
                     LGLRLLVPGALCYALARVLWPVIQAGGEAMSLIRVMLLTVMVDAGLCAILIPAGGAAG
                     AACATSLSFALVAGGYLWILRRRNVSVFEGLALSRLVGLVVMTGIAMAGLSALVATPM
                     LSLLVGIAVGVFLYWGGVFSLGIVQVDELESLVQSLPGTFRKAGESVLRMVAPLLLRL
                     KAAASN"
     assembly_gap    38172..38271
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<38272..38777)
                     /locus_tag="PK589_09835"
     CDS             complement(<38272..38777)
                     /locus_tag="PK589_09835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ19943.1"
                     /translation="MRKMPHVCILTSQYFDWGIYGGFGSMSRKLAESLVRAGHRVSVI
                     VPGRQGQQPTETIGGVEIRSFSPRNVMDACRLIRESRADIFHSQDPTVLTYLAQRIHP
                     RRAHLVTSRDPRELSDWWVEFLYATPMRRLLTPLNYFTESGFLVRQAVRRADAVYCPA
                     HFLKEKVKR"
     gene            complement(38774..39985)
                     /locus_tag="PK589_09840"
     CDS             complement(38774..39985)
                     /locus_tag="PK589_09840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ19944.1"
                     /translation="MFDNEFPPLGGGTGVVNLHLLEEMALRPDVTVDLVTSSRTKARY
                     ETEAFADRITIYKVPVDNRNIHHATNRELLRYSWRGLRLARQLASEHQYDVSFAFAGV
                     PAGAISYLLRLTHGLPYVLSLQGPDVPGFEARYNYLYPVLTPFITQIWRRAGVVTAIS
                     AEQVALAHRTIPDLPLITIPNGVDTNAFVPATRNSRRPFTMICVARLIERKGQHHLLQ
                     AFANLRAANHEPMALIFVGTGDAEPQLRELAAKLQVAEAVTFKGFVPRENMPPVYHDA
                     DVFVLPSQHEGMSIALLEAMASGLPVIVTDTGGTAELVTQGANGEIVPWDDVEALTTA
                     LQRLLNAAEQRRRMGDESRRRALAFGWPTLAARYLELCARVMVPSAESRAKDARSAKN
                     VARDWTRELRG"
     gene            complement(40003..>40353)
                     /locus_tag="PK589_09845"
     CDS             complement(40003..>40353)
                     /locus_tag="PK589_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ19945.1"
                     /translation="PYERQARHAGTPKYTLRKLIYLALDGLVSFSHMPLRIITLLGFT
                     VSLLSFLVALFYLIKKLTIGVGVAGFTTLVVSIFFLAGIQLMTIGVIGEYIGRISDEV
                     KHRPLYVARRVTRR"
     assembly_gap    40354..40453
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(40609..41604)
                     /locus_tag="PK589_09850"
     CDS             complement(40609..41604)
                     /locus_tag="PK589_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNJ19946.1"
                     /translation="MLKAALLLLGALTLSALVWHIGIGRIYDAVTQLGPAAMVLVLLP
                     SLLMYVLEAYGWRVTLGAWAKGVPFWRILAIRTAGEVVNMTTPTAYVGGEPLKAYLLN
                     RHGVPLVEGLASVVTAKTTMTIAQILFILAGIGLGFWLLGSGGSAGQTITAGLVSVGL
                     LLFGVGAFVIVQRQGMFGWILALLRRIGLRIQFLEAREQQLLELDRTIAGFYSHQRQA
                     FLLSTGLFLLGWLAEALEVYVMVLCMGQSINVLPAVAIGALSVFIKGGTFFIPGSLGA
                     QDAGNLFLLSAFGYGEVTGITFALLRRFREFVWIALGLGCLAMMTKGADPSPSQK"
     gene            complement(41597..43051)
                     /locus_tag="PK589_09855"
     CDS             complement(41597..43051)
                     /locus_tag="PK589_09855"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNJ19947.1"
                     /translation="MSESTLQRGAELQGLSTAILLPGAGLFGDRPGRGLADVGPLTHI
                     GGLSLFQRTVLTLQRGGIRQLIVLAGPDEELLKQALARGARVTIPVRWMPVREFPLDD
                     PRTWESLAAEVRGFCLVAGVQAVFSKGLIEHLRQTVRDGEALVVTREAGSIEPAAGRR
                     NPVVAFQEGRLVSFHNQPVQQGHQVAADLVVLPASILNPPSGAAAPLTSGLAEAPGMT
                     PMRRWLERAAIEGRVRVVAAASHAGLWYREVWDQASAGHAERTLFRSLKGEAEGFVDR
                     YFNRTFSRLLTRLFLQLKCSPNTITMVATAVGLLSAVGFGLGTYTAGIVAALLFQLAA
                     IIDCCDGEVARLTFTESPFGAWLDIAMDNVVHIAIFAGIACGAYLRHADAPEAWVSLA
                     LGSAAVFGNAMAFWLVTKAQKIGATRGWNTPKQAAWSDFILKNVASRDFSVVVFLFAV
                     ADKLDWFLWMAALGSTVFWLMMVWVIRPSARARA"
     gene            complement(43104..43823)
                     /locus_tag="PK589_09860"
     CDS             complement(43104..43823)
                     /locus_tag="PK589_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HNJ19948.1"
                     /translation="MKAIILAAGVGKRLWPVTQHKPKCLIEIGGQTLLSRYLESLASV
                     KIRQATIVVGYKQEMIRAAVGSQCHGVDISYLVNEEFHRGSISSLWIARQALTDDVVI
                     MDADVLFHREILRRLVASPYENCLLMDDTVKQTGEECMVVVQGDRVIALSKKVPDRYD
                     LAGEGVGFLRVRRADTAHVIASLKGYIDQDRWDMEYEDGLLQYFRDVKVGHEKIGGLP
                     WTEIDFPEDVTKAEREILPRL"
     gene            complement(43820..44914)
                     /locus_tag="PK589_09865"
     CDS             complement(43820..44914)
                     /locus_tag="PK589_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNJ19949.1"
                     /translation="MILLNPGPVNVSERVRQALLRPDICHRESEFSDMLQRIQQKLLT
                     AFVPGAESEYVAVILTGSGTAAVESAVMSCLPLGKRMLVLNNGVYGERLSNMIGLQRL
                     GVSELKSEWHVRPDPERLRLALRQHPEVHAVSMVHHETTTGLINPVKEIAEVVDSLNR
                     VFVLDSVSGLAGEPIDIAGSHIYMVAGTAGKCIQGFPGVSFVLLRKGFLDQMRKYPKR
                     SWYLTLTHYVDEQGRGIVPFTPAVQVYYAFEEALDELLEEGVTSRFHRYRRMATRIRE
                     RMAGMGVQPFLPADVQSNTITAFHLPAGLSYTTLHDQLKARGYVIYAGQGQLESKVFR
                     VANMGALTESQINGFLGAFEEVLAGAAARA"
BASE COUNT         9680 a        14507 c        12045 g         8509 t
ORIGIN      
        1 aactatgatc gaagtctata agacgaccta aaaactgtca aacaaatgca cgaaagacga
       61 agcccggtaa aaggccatag gagcggcgag cggtgaaggc acgctatgcc tggctggagg
      121 taatgcgtac gtatccgcgg gagaggtagt gcaggccgga ggtcagcgtc acaccgccca
      181 tcaggtacaa aagcggatcg agcgtggaaa gatcaagccg ccgcgaggca aagaaaacga
      241 ccgccaccaa cgtcgccagc tggatcaatg tcgtgccttt ccccagcacc gtcggcgaga
      301 tatcgacctg cgtatgagtg aagtgggcga ccgccgcccc caacatcagc atgaggtcgc
      361 ggctggcgac gagaatcgtc agccagagcg gcacgagatg catgacagag agggtgatga
      421 atcccgtggt cagcatcagt ttgtccgcca agggatcgag gacttccccg agcctcgttc
      481 gctgatctgc cacccgggcg atgattccgt cgagcgcatc ggtcaacccg gcgatgaaca
      541 ggaccgccaa tgcatagtcg aattgctcgt agttcagcag cccgacatac acgggaatca
      601 acagaatgcg taacatcgtc agactgttgg gaacgttcat attcatcaca agaaactgac
      661 gggacacacc atttcggttg ccggatcacg gcacaggaat cgcgcatcat agaaacccct
      721 gcggaacctt gtcaacgacg gttgcactcg acaaggcagg tccctataat cgggacccgt
      781 aagtcgcggc ctacagatga tcgacggagg tttagtatga gtggactccc cctcctcttt
      841 aagaaagagg gattaattga gcggcatcag gtcgagggga tcgatccaag cgatcgctat
      901 tttaaccggg ctgtcctggt gaaccgggtc gcggccggat acacgggcaa aatgacctac
      961 gaagcctacg ccgtggaggg taccccccat tcaacgacca gtgccgccgt caaagccgtc
     1021 gtcgacaagc tgcagagcgt gggtttcact cggatgcgca cacgcctgaa cttcaaaggc
     1081 aatcgctatc tcgccgaaaa agaaacctgg actgactacc ccgatttgtc cgcttaattt
     1141 ctcagtaatt cccggcaaat aacatcgtgg atgtgcgggc ggaagacccg gatctgttca
     1201 ttccgcctgg tagggtgaac ccgattggag aggagcacca cttccagctc cttgaccgga
     1261 tcgatccaca atgacgtgcc cgtgaacccg agatggccga acgactcgag agagaaacac
     1321 gttcctgacg acgaccgcac cgacggcgtg tcccacccaa gcgcccagct ggagttcgga
     1381 acaccctgcc tgtttgtcgt aaaggtcctg acgatctcgt gttccaataa ccctcgtttt
     1441 ttgtgccagc catccatcca ggcttgagaa acggccagta cggcccgggc agtcccgaac
     1501 aaccccgcgt gaccagcgac tccgccaaga gcataggcat tttcgtcatg gacctcgcca
     1561 cagagcatgc gcttccgcca gggatcctcc tccgttggag caatgatgca ttctgcatcg
     1621 taatggataa acaaggccga taggggccca cgccgcgcat aggccaacgg gcgagcgcca
     1681 agcggctcgt agatctgccc actgcagaat tgatccagcg gttcgcctga gagccgttcc
     1741 acggcgaagc ccaacagcat aaaacccaga tcgctataca agctgcggga gccccgctcg
     1801 taaattaact cttccttcgc gatgtagccc gttaccgcct cgcacgccgc tggactgccc
     1861 atgaatcccg gctgcgtcgc ctcgatcgcg gcaatacgtt cgtacaaggg gcgccatccc
     1921 ggcagaccgg aactgtgggt gagcaactgc cgcaccgtgg cggctccgat cgcactgtct
     1981 cgcaactcat tcaagatccc gtcaatgcgg tcgtccacct tcaggcgtcc acgctggatc
     2041 aacaaggcga gcgcggtggt cgtcgcgaga actttggtca gagacgccag gtcgtaacag
     2101 gtatgatccg tgacggcttc ctccgaccct tgccgggaca gccggccgac ggcgctttcg
     2161 aagacgatct ccccgcgcaa gcgtactgcc agaacggcac ctggaaatgt cccgtcgtcg
     2221 acggcggctt gcagcgccga ctgtatcgga tcgttcatag ccatccgtac gatctagcgt
     2281 gaagctgagc tggcggggag aggccgggag cggacggtcc gctccgccgg ttcaggattg
     2341 caggcaaagg tggaattccc gatgaacgca ggagactcaa cgcgcagggc tggattcgcc
     2401 ggcgagcttc ccttcaaccg aagccccctc tgcttgatac gcgtcgctct cttcaacttc
     2461 cagcatccgc tcaaatgacc gcagggtaga acgaaagcgc tccaccatca tgagccgctg
     2521 tttttgcaga tccgccacgc cgcgttgcat ctcggtgaga ctgccgcgcg cctgccggat
     2581 tatttcactc gctttcagtt cggcctcctt cacgatcaag tcggcctctc gttgggcgga
     2641 tcgcttgacg tcctccgcca gcgtctgggc cgagactaat gtgttggaga gcgtcgcttc
     2701 ggtgcgtcgc aagtcagcca cctgctgctc ggtggtggtg agtttttcac gaaggaggct
     2761 attctctcga ttcaaatttt cgacggtgag cgccaactcc tcgagaaagc ggttcacttc
     2821 atcccggtca aaccctcgaa acttgacctg aaacaccatc tgctgaatat cgatgggagt
     2881 gattttcatg cgacacctct tgcccgacag ttaatgcatc cgcacggcaa tatcccgcaa
     2941 ggattgaacc acggcatatt gcaaaaagac aaggatcagg atcgcaatca tcggcgaaag
     3001 atccatgccc atgcgccatc ctatcagccg ccgaatcggt gtgaggaccg gttccgtcgc
     3061 gcgctccaaa aattggacaa tcggattcca cggatccgga ttcacccatg atattaaggc
     3121 acgggcgatg atgacccaca tgtagagcca caacaccgta tccaacactg ttgccgtccc
     3181 ttgcagcaca ttgctcacca caaacatcag cgtcccaact cctgtgaacg ttttgtcgcc
     3241 gcctcgacgg ctgccatcaa acatccccgc atgccgccga cttccaattg atgcaaccct
     3301 gcgatggtcg tgccacctgg ggaggccacc tggtccttga gctgagccgg atgctgccct
     3361 cgttccagaa ccatccgtgc tgcaccgagc accgtctgag ccgccagcaa ttgtgcggtc
     3421 gcacgcggca agcccatttt cactccgccg tctgctaatg cctcgatcgc cacgaacaca
     3481 taggccggtc cgctcccgct caacccagta accgcgtcca tcagccgttc ctcgaccgag
     3541 accacagagc ccaccgcttc gaatagagcc cgcaccgccg ccacatcatc agcggcaagg
     3601 tcagccgcat aggccaatgc ggtgacccct tcgcgcacca gcgccggcgt attcggcatc
     3661 gcccgcacaa tgcctctcgc agccgagacc cgctcctgaa tccacgccgt cgtgacaccg
     3721 gcagcaatcg aaaccacgag tttccctctg agagctgaac tcacgtcctg gagcacagcg
     3781 ggcaacacct gaggcttcac agccaagaga acgagatctg cccccgcgac agccttggca
     3841 ttatcttcac caacctgaat gccaaaacgc gacttgagga gatcccgccg ggccgaaacg
     3901 gggtctgtgg cacagatgaa ctgtggctca ctgaccttcg aggccaacag accaccaatc
     3961 aatgcctcag ccatctgccc gccgccaaga acggcgatgt tacgtgtgat catgatacgg
     4021 ctcactccgg gccccgaaaa ttgcggtgcc gactctgacg tacgtcgcgc cttcttcaat
     4081 ggcgaccgcg tagtcatggg acatgcccat cgacagttcc tgcatattaa tgttccggca
     4141 gccctgcgct gtcaatgcct gcgcgagttc gcgaagctgt cggaagtagg gacgcgcgtc
     4201 ctccgcagtc ggcatgggcg gaggaatggc catgagacca cgaatatcca actgctccaa
     4261 ttggttcaga gcgggaaggg ctgccgccac tgctgcaggg gcaaaaccgc ctttgctgga
     4321 ttctcctccg agattcacct ccagcagcac ccgttgcttc aaccccgctg ccttggcctg
     4381 acgatcgatc tcttccgcca gggcgaggct gtcgacagag tgaatggtct cgaaccgacc
     4441 gacgaccgac tttactttcc gtcgctgcag ggtgccgatg aaatgccaga cgaccccttc
     4501 ccgatccagc gtctcgattt tcgggagcgc ctcctgcagg cggttttcac ctagatgtcg
     4561 aataccggca tccacagcct cccgcaaccg gtccacggtg acggttttgg atgccgccag
     4621 cagccgcacc gagtccggag cccgtcccgc acggaccgtg gctcggtgaa tctcgtcgaa
     4681 caccgctcga acccgaccgg caattgtgcc cagctctgaa tccatcctgc cgccttgcag
     4741 attgccgctt tcgctcaact ccgatggatc ccgggcacct gcaggcgcgg agattcagga
     4801 tgacaactag ccgcccccgt taccgtgagg acacgagggc aatcccgctg accatcgtcg
     4861 ctttcaccac accttcccgg cgatagctga aaaactcgtc gggctgacag atagtgcagg
     4921 cctcaaccgt ggcaatccgt tctccctcca acccttcatg catggcttga cgccggacga
     4981 acgcccgcaa attcaaatgc gctttctgag gatttactgg cgatacgacc gactgccact
     5041 cagggaacac gtccctgagt cgtgtcagaa ccggttcgtc gacctcataa caacaggcac
     5101 cggcggaagg cccgatggcc atgcgcacgt ctttgggcac ggtcccgaat cggctggcaa
     5161 gcaacgcgac agtcttcggc acaatgcctg ccacagcgcc acgccagccc gcatggatgg
     5221 cagccacgac ctgtcgcaca ggatcatgca acaggacggg tacgcagtcg gccgttcgca
     5281 cggtgaccat cagtcccggc tgattggtga cgagcgcatc ccagcccccc tcgaacagct
     5341 ccccctgctg gaccggccga tcgacgacca aggcatcggt cccatgcacc tgcttgacgg
     5401 aaaggatcac gggcgctctg tgatcctgtt gttccgttct gtgcgcagag gcacgtcccg
     5461 gcgtgaccgg tatggcggac agccgggtgc cgaaaaagtg ttctgccccg tcggcatggg
     5521 ttgcaaacga gggaagggta atgaactctg acgccatcga ctcctgcgac cttacgtctt
     5581 ccggaacgga ctcatcttca atcggcttgc ttgcgcagga aggtgggcac atcccactca
     5641 tcgtcgccga caactgcgat tcgctccacc gcttcccgcg tctcacccat gcgccggagg
     5701 aaggtcggcc gatcgatgtc cttgatgggc cgatcggatc ctgccgcatg gacaccggtc
     5761 aacacctgct gggcaggacg cccgttggga gtccgtgcgg cggggcgctc cgcggcggca
     5821 gccggtcgag cagccggttg ctcttcccgt tcgaatcccg tcgcaataac cgtcacgatc
     5881 agatcgtcgc cgatttccgg attgatcacc tggccgacga tgatattcgc ttcggcatcc
     5941 gccgcatgtt gcacgatcga cgcggcctct tctacttcat gcagcgacat attcggccca
     6001 ccggtgatat tgagcaacac cccgcgagcc ccttccacgc taccttcttc cagcaacggg
     6061 ctgcaaatgg ctttctgtgc ggcctcttgt gcccggttgg cgccgcggcc gatgcccatg
     6121 cccatcaccg cacgtcccgt atgggccata atagtccgca catcggcgaa gtcgacgttc
     6181 acgagtcccg tggttgtaat gacatcggca atgccctgaa tggcctgtcg caacacatca
     6241 tccgcgacct tgaacgcatc cagcaacggc gtcgccttat ccacgatgcc cagcaaccgt
     6301 tgattcggga tgatcagcag cgtgtcgaca tgccggccga gatcgcgaat gccttcctcg
     6361 gcgtggctca tccggcgatg cccttcatat tgaaacggtt tcgtcacgac ggcgacggtg
     6421 agaatcccca gttcacgcgc aatactcgcc acgatcggcg cggcgccggt cccggtgcca
     6481 ccgcccatcc cggcagtgac gaagaccatg tcggccccga ccaggctctc ccggatctca
     6541 tccttgcttt cgagggccgc atcgcgaccg acctcaggct tggcacccgc gcccaggcct
     6601 cgtgtgcgct ccggaccgat ttgaatttta taggaagcct gcgaccgttc gagcgcctgc
     6661 acgtcagtat ttgccgccac aaagtcgacc cggcacagtc cgccggtgat catcgtattg
     6721 acggcgttgc accctgcgcc tccgaccccg atcactttga tgcgaacggg cgattgcggc
     6781 tcctcttgaa atgaaaacat tgtggcacct cccttcacga tggcgcggat ggtctgaagc
     6841 cgtccgcaac agttagaaaa attcgaacat ccacgatcgc atgcgatcaa acactttacc
     6901 caaccctttg ccgtgacgaa tgccggcagc ctccaagttg tcggcatgtt gacgcgcgtg
     6961 gagtaacagc ccgacgccgg tcgcatgcat cgggttgctg acgatatccc gtaatccacc
     7021 gatacctgtc ggcgcgcccc ggcgggccgg cagattcagc ccgcgctccg ccgcgtccgg
     7081 catgccttcc aagagcgagg ttccacccgt aatcacaacg cccgcgccga gcatgccttc
     7141 atacccggcc cgcacgattt cgcgcttgac gaggtcgaac atttcttcga cgcgaggttc
     7201 gagaatttcc gcgatgtcgc gacgcgtgaa ttgccgcggc ggacggtcgc cgaccgaggg
     7261 cacttcgacc atctgatgcc cgtggaccag ctcggtgcgg gcgatgccat gttgcacttt
     7321 gatcttttcc gcgtcggtct gcgaggtcaa caagccaatg gcgagatctt tggtaagatt
     7381 ctgtccgccg atgggaagga ccgccgtgtg ccggatgctc ccatcgagga aaatggcgag
     7441 gtcggtagtg ccgccgccca agtccaccat ggccacgccc agctcgcgtt cctccgcgct
     7501 caacaccgct tcgctggagg ccagcggctg caggatgatg tcaaccacgt cgaggcctgc
     7561 ccggttcacg ctcttgatga tattctgggc cgaggtcacc gcccccgtga tgacatgcac
     7621 attgacctcg agccggttgc ccgacatgcc cagcggctcg cgcacccctt cctgatcgtc
     7681 gaccatgaac tcgcgcggca acacatgcag aatgcgccgc tcatggggaa tgaccgccag
     7741 cgttcttgcg ctctccacgg cacgactgat gtcatcgcgc gtgacttcct gtttcttgag
     7801 cgccacgacg cccttgaggt tctctcccga aatgtggctg cccgcaatgc cggtgtagac
     7861 cgaattgatc tgcacggccg ccatgagttc cgcttcctcg accgctttct tgatcgattc
     7921 cacagtactc tcgatattga ccacgacacc cttgcgaagc ccgcgcgaag gacaggagcc
     7981 gacgccgatg atgttcagcg ggccctcctc cggtacttcg gcgacaatgg cgcaaatctt
     8041 ggtcgttccg atatcgagcc cgaccaggat atgatcccgc ttagacactc ggctcacccc
     8101 cttccccgaa caatgacgcg gtcggcaaag cgaagatcga tttcattcgc tctggcgccc
     8161 tcgccgtcaa aggccacatc acgcagtgcg ggccgcattt tcaaaaaccg agaccattgc
     8221 tgattcatcg atgattcact gaactgaaac gtgacaccct gtacctcaac gaccagatta
     8281 ttcaaattcc ccacattgat gtccggccga ccgccggtgg tctgccccac catgcgcgcc
     8341 aaggccgccc ctacctgcac ggggcggcga tcctccgccc ggccttgtgc caaccgcttg
     8401 ccatcgaccc ccgacaggat gggaagcgtc gggtcatcgc tggaccccag acgcgccagc
     8461 aacacccctt cggcatcggt caacaaattt tccgccagcg tgcggacgac caccgccggc
     8521 tcccgctcga cgatgtcgat atggatttcg tgcaacggct tcagcacgac ggtcgcatcc
     8581 ttgatccagg gatgagtctt gatccgttcc gccaaccacg tcggattgat ggaatagagc
     8641 gcggtatccg gtttgagcgc cagccgaccg atcacctctt ttctggtcac gtgatgcaga
     8701 ccattcaccg acacggagcg caccagaaac cactcgcgcg tcagcggccc tatttcgcgc
     8761 gccagcacaa agagtcccga acagccaccg accaggaccg tcaccgtggc gatgactcgc
     8821 aaggtaatca gcaggcctcg cccaagccgt gtccaatacc cgcctgtcgc tccgccacga
     8881 cgacgttcca ggcccgattc cgaacgggca ttcgcccgcg gattgagttt ggccggtcgc
     8941 gtgttccgcc acctgagcgg catcgttacc tcacggctcg aaatgatccc gttccagccc
     9001 gccgaagggc ggattggagg atctgctccg tcaacgcatc gtatgacaga ccggctttgc
     9061 ctgccgccat tggcaacaga ctcgtctccg tcattcccgg gaccgtgttg atttccagca
     9121 catagggttt gcctttggcc gtaatacgaa aatccacccg cgccgctccg ttgcagccca
     9181 aagcctgata cgtgcggatg gccaattgct tgatgtcgcg gctcacagca gcaggcagtg
     9241 gcgccgggca aagatactgc gtgcggccct tctcgtactt ggccgaaaaa tcataaaatc
     9301 cgtccggtgc gacaatctcc acagcaggca atccggcaac gctcccatca gcagtcccta
     9361 acaacgacag cgtgacttcg tgcccgggga taaacgcctc gaccatcgct tctggatcgt
     9421 atcgatgggc caccttcagt gcctctttcc actgcgaagg ttggcgaacg atggtgacgc
     9481 cgatggtcga cccctggctc gccggtttca cgacgatcgg caacttcaat ttgcagctcg
     9541 tcaggatacg attcaacgtg ggcgctgtgc cgcgccacac gaccgtgccg cgcggcaccg
     9601 gaataccttg ggcggccagc tctgttttgg tcaccacctt atgcatgccg atggcgctgg
     9661 cgcgcacgcc agatcctgtg tagggaatac cgagggtttc cagaaatccc tggatggcgc
     9721 cgtcttcccc gcctggtcca tgtaatgcca ggaacgccac ctcgatcgcc tggtcttgca
     9781 aggtctggga caaccccggt cccacgtcga ttgccaccgc atcgtaaccg ctgcgcacaa
     9841 gcgaccggta cacggcttcg cctgtcttga gggagacttc gcgttccgag gactgccccc
     9901 ccatcaacac cccgattttc gaacgagtca gcggcttccg ttccgtcaca tgcactccct
     9961 tcatcgatcg gcctcaggcc tgaccgacca ccttcaattc caattccagt gtgacgcccg
    10021 aggccttctt cactcccgcg cgcactttcc tgatcaaggc cagcacatcc gccgcgcgcg
    10081 catgcccgag gttcaccatg aaattggcgt gcttctctga gacctgcgca tccccgacac
    10141 gcgcaccctt gagccccgcg acgtcaacta aacgcccggc ggaatcctgc ggaggattct
    10201 tgaacacgca gccggcgctg gggagcgtca agggctgggt gttcttgcga tactgcaaat
    10261 actctttcac ggtcttttcg atctgatcgt gcgcgccggg cttcaattgc agccacactg
    10321 ccgctacgat cccgcgcggc agatgtgcct tccggtaact gaacgggatc tcggcggccg
    10381 gaatgtccat cacccgcccc ttcggatcca ccatgcgcac ggccttgacc gaatccttca
    10441 tttcacccaa gcgcgtcccc gcgttcatca ccacacaacc ggcgaccgtg ccgggaatgc
    10501 cggcgcccca ttccagccct gccagcgagc gacgtatggc atacccgatc aacgtcggca
    10561 tgcccacgcc cccctccgca tagaggacag atcccgcctc ctggcggatg gccttgagct
    10621 gccggaggct aagcacgagg ccgcggattc ccccgtcacg aacgagcaga ttcgtcccac
    10681 ccaccacgaa aatcgggagg cgttcggcgt gagcctgcgc caccacccgg cacaaatcgt
    10741 ccacatcggc cggctccacc aggacttcgg ccggtccccc gatgcggaac gacgtatagg
    10801 attgcagcga cgcctcgtaa gtgatcgttc cacgaattcc ctcaaggatg cgcgcccagt
    10861 ctttcctcgt tctcgccgat tgctctttcc gcgccacacg tgacaccgtt cctgcctctc
    10921 atcgccctca ggccggcaat cgatcgagga tgcccaatcc cgccttccaa atatctcccg
    10981 cgccgagcgt gatcacgaga tcccccgatt tcagcgtcgg cagtacctgc tcggccagag
    11041 tttccttccg ctcgacgaag gtggccgaag gatggcccgc agccttcacc gcttcgacca
    11101 acttggcgcc ggacacgccg ggaatcggtt gctcgccggc ggcatagatc tccgtcataa
    11161 agagcgcatc ggcttgatcg aatgcatggg aaaactcctg catcaaatcg cgtgagcggc
    11221 tatagcggtg aggttgaaag agcacgacca cgcgccggtc ccagccctgc ttcgcagccg
    11281 caatcgtggc ccgcacttcg gtcggatgat ggccgtaatc gtccaccacc atgatgccgg
    11341 ccttttcccc gcgcagatga aaccggcgtt cgacgccgct gaacgctgcc aggcctttcc
    11401 ggatgagatc aaccggaata tccagttcca tgccgatcgc aatcgcgacc agcgaattcg
    11461 acacattgtg gattccggga atggccaacc ggaacggccc caggttgcgt ccccggaaga
    11521 acacccggaa ctcggacccc cattgccgca aagcgatgtc ggtggcacga aaatccggca
    11581 cgtgatcggg atattcgttc agcccgtaag tctggaatcg tttcaccacg cgcggcaaca
    11641 gattgcgcag ccgttcgtca tcggagcaga gcacggccag gccgtagaac gggaccttgt
    11701 tcacaaattc gaggaaactg tcgttgagcc gctccatgct gccgtaatga tccagatgct
    11761 cacgatcgat attcgtgacg gcggcgatgg tcggcgccag acggagaaac gagccgtcgc
    11821 tttcgtcggc ttccgcgatg agcagatcgc ctcgccccaa ccgggcatgg cttcccagcg
    11881 cgttcacctt gccgccgatc accatggtcg gatccagccc gccctgtgcc agcacattgg
    11941 ccaccattga cgtcgtggtg gttttgccat gcgccccggc gatcgccacg ccgaacttca
    12001 gccgcatcag ttcggccaac atttcagcgc gcggaatgac cggaatctga cgcgcttttg
    12061 ccgccaccac ctcaggattg gtcgccgcca ccgcggagga aatcaccacc acttgtgcct
    12121 cgccgatatt ggcctcatga tggccgatgg cgatgcgccc tcccagctct tcaagccggc
    12181 gcgtcgtttc cgactggctg aggtccgacc cgctgacttt gtatccgagc gtcaacaaca
    12241 cctcggcgat gccgctcatc ccggatccgc cgatgcccac cagatgaata tgttgtgtct
    12301 ttctgaacat cgccgtccgc cttagctcct gtcctcgaaa tgatcgctgt ccgctgttcc
    12361 gatcctgttg ctgtctctcc cgctctcgtt gttcataggg cgcgagcgct gtcgctggcc
    12421 tcatggcgcc tccttatgac gtcataacat tcacgcacaa tggtttccgc cgcatcactg
    12481 cgccgcatat tccagctgtg ccggctcatg gtctccaaac gttgctgatc gctgaaaatt
    12541 tccgtcatgg catgggccag agccgcaccg gtcaattccg cctggggcag aaggactgcc
    12601 ccgcctgcct tcgccatcac ttctgcgttc cgcaactgat gattgtaaat agccgtgggg
    12661 agcgggatca gaatcgcggg cttcccgcag acagtcaatt cggcaatcgt catggctccg
    12721 gcccgcgcca ccacgagatc tgcggcacga agcgcggtgg gcatgtcgta cagaaacggg
    12781 accacctgcg cagagattcc tgcttgctga tacgccgccg caactcgcgc atgatccgcc
    12841 tcgccggttt ggtgcgtgat ggtcaaccgg gtattcatgg tggcgagcat aggcaacgcg
    12901 tcgatcactg ccgagttgat ggccttcgct ccctgactgc cgccgaaaat caacaggtgc
    12961 cgcttcggtc cggtctcgct ccgtgccgtc tcgggagagg acgaatccaa gaaggcttgc
    13021 ctcaccggca ccccgaccac gctcgtcttg cgtcgatcga aaaattgttt cgtggactcg
    13081 aaggccagaa agatgcgctg cacgagcggc gcgacggctt tattcgccag ccccgggtag
    13141 gcattgggct cgagaatcac gccggggact cggctcaaaa acgccgccac cagcatggct
    13201 gggctggtgt atccccctac gccgaacacc agatcggcgc tctgctgttt cagcacgccg
    13261 agcgattgcc aaagactcac cggcagagtg atcagagctt tcaccatctc aaccgggctc
    13321 ttgcccataa acggatgcgc cgtgatgcac cgcaacggaa gcccttcatg ggccagcacc
    13381 ttttgctcaa tgccacgcgt cgtcccgacg aagaggatgc gggtcgatgg atctcgccgc
    13441 aaaaattcac gcgccacggc gatggccgga tacagatgcc cccctgtgcc tcctgctgca
    13501 atcacgatgt tcattccgtg atgaccccgc gctttcgcgc gccccgtgat ccgccgcctt
    13561 cttgcccgcc atggccgccc tgccggtcgc gtgagatatt caacagaatc ccaacgccga
    13621 acagattggc taccagcgaa gagccgccgt aactgacgaa ggggagcgtc aaccccttgg
    13681 tgggtaacag tcccgtgacc acacccgcat tgaccagcgc ctgcatgccg atcaacatcg
    13741 tgatgcccat cgccagatgt tttccgaatg cattcctcgc ccggcccgca atctgaaagc
    13801 ctttgatcac gaagagaccg aagagcaaca cgatcgtcac ggtgcccaac aaccccaact
    13861 cctcgccgac caacgcgagc acaaaatctg tgtgcgcctc cggcaagaag aataatttct
    13921 gtttgccctc tcccaagccg actccgaacg gcccaccgct tccgaaggct aaaaacgact
    13981 gcgtgatctg aaagcccgct ccggaaggat ccttcgtggg atccaagaac atgagaaacc
    14041 gtttccaacg gtacggtgac gtcaaaatga gcccggccac cccagttaag gcgcacaagg
    14101 agagaatcgc caggtgcttg atccgcgccc cggccagaaa cagcaacgtc accaccacga
    14161 gtcccatcac cacgacggtg cccaggtccg gttccagcag caccaagccg ctcagcaaac
    14221 caatgacgat cagcggcggc aacaagcccc gcgcgaattg cgtaatctga tcctgctttt
    14281 tggtcagata ggcagccgca tagatgacgg ccacgaattt cgccacctcg gccggctgaa
    14341 tattgatcgg ccccagatgg agccaccgcc gtgcaccctt ggccacattc ccgagcgatg
    14401 gaatgagcac caacacgagc aacagcgtag ccccgcagag cagggggatc gccagcttct
    14461 tccagattgt gtagtcgatc cgtgagatga gatgcatgac cagcaggccg acgcccaacc
    14521 aggcgacctg ccgtttgagg aaataccacg gatcgtgaaa ccggttgccc gccacaacgg
    14581 cactggcgct gaacaccatc accacaccga tcagggccag gatcagggtg accgccaaca
    14641 gtgccgggtc caccggcacg cgcttggacg cccgctgagt cgaagtcgac cagggcaaca
    14701 tcagagtccc gagcgcatgc tgtgccatcg ttcagttgtc gttcctccgc gcgactcccg
    14761 gtctcacgcc ggaagcgcct ggaccagcgc cttgaattgc cggccccggt cttgataatc
    14821 cgcaaacatg tcaaaactgg cgcaggccgg cgagagtagg accacctcac cgggctgcgc
    14881 ctcgcgagca gcaagctcca cggcatcgcg gagggtcgct gcaggacgac accggtcaaa
    14941 atcacccatc gccgcttgga tgcgggctgc ggcttcgcct attagaatga gccccttcac
    15001 ccgttcacga atcgctcctt caagacggga gaaatcaccg cctttatcac gtccgccggc
    15061 gatcaaccag atcggctgtt cgataccttc cagggctttc aataccgcat cgacattcgt
    15121 acctttggaa tcattcacgt accggacgcc ccggcgctca cgtaccactt ccaaggcatg
    15181 ctccaggccg ggaaagaatc gcagtacggc gcggatcgct tcgatggaac agccgcacag
    15241 cagaccatag gtcactgacg ccatcacgtt ttccacattg tggagtccga tcaatcgcat
    15301 gtcgccccgg cgacagattt cctcgcgctg tccccgcacc gtcgtcacga tcagatcgcc
    15361 gtccagcacc gtggcgccgg ccacgccgga aaccgtggct ccgctccggc tgaagccgag
    15421 gacggcagct tttgtattcc cgcgcagcgc agcgactcgc gtgtcgtcca gattgaacag
    15481 ggaatagtca ccggccgttt gattcgtgaa aatcctcgcc ttggccgcca cataatcttc
    15541 taccgagttg taacggtcca tatggtcgag cgtcacgttc aagatcgatg caatgaacgg
    15601 atggaactgc tcaattgttt ccagctgaaa gctcgaggtt tccaagacca catactcata
    15661 aggcggaggg ctcccgggct tggcctgcac acacgtgaga gccgcttcgc tggctgcgat
    15721 gccgagattg ccccccacga aagctcgctt tccgctttcc ttcagaaact tgccgatcaa
    15781 cgtcaccgtg gtgctttttc cgttggttcc ggtcaccgcc acgatcggcg cggtcatata
    15841 ccgtgacgcc aattccagtt cgccgatcac tcgcaccccg cgagcacgca cacggttgag
    15901 cgcatccagc tgtgtcggca ctccggggct gatcacgacc agatccgcgc cctcgagggc
    15961 ggattcatac tgggctccca ccttgacggc aatgcccgac tgatccaact gcgagaggat
    16021 ggtcgcgagt tcctgcggct ctttccggtc ggccacggtg acccgtgccc caagatggtt
    16081 caacagccgc gcagcgccga cgccgctcct ggcgagacca acgaccgtga cttgaagacc
    16141 cttgacgtcc atacgattcc gtctaccgca atttgagcgt gctcaagctg agcaacgcca
    16201 acaaaatggc aataatccac aggcgcacga cgaccttcgg ctcgtcccat cctttcatct
    16261 caaagtgatg atggatcggc gccatattga aaatgcgctt gccccgcaat ttataggatc
    16321 caacctgcaa gatgaccgac aaggcttcga tgacgaacac tccgcccacc aaaaggagca
    16381 gcagttcatg cttgctgatc acggccaccg ttcccagcgc cgcgcctaag gggagcgacc
    16441 ccacgtcgcc catgaatacg gaagccgggt acgtgttgaa ccaaaggaat cccagactcg
    16501 accctaaaat cgcacccgta aaaatagcga tttctccggc gccttcgatg tacgggataa
    16561 gcaaatattc ggccatgacc cgattccctg tcacatacgc gacgatcgta taggccagtg
    16621 aggcgatcat gacggggccg atcgccaacc catcgagccc gtccgtcaaa ttgacggcat
    16681 tggaactgcc gacgatgaca aggatgacga acacaatgta gaaccagccc aagtccggcg
    16741 taaaaaactt gaagaagggc acgctgagtt tggtcgtgta gctcgggagg gtgtagagaa
    16801 agacgccgat ggccaacgcc acgaggaact ggccggaaaa tttttgcgcg gccgacagtc
    16861 ctttggattg gcgcttgatg aatttcagat agtcgtcggc aaatcccacg gcgccgaagc
    16921 cgacggtcgc caccaccacc aaccacacgt agcggttggt catgtcggcc cacaagagcg
    16981 tggagagcac gaccgcaaag ataatgagaa tgccgcccat cgtcggcgtt ccactcttgg
    17041 ccaagtgccg cttcggcccg tcgtcgcgaa tctgctggcc gagtttaatc tcctgcaatt
    17101 tccggatgac ccagggcgcc atcacaaagg cgatgagaaa cgcggtgacc gctgcgtaga
    17161 tgatccggaa actctgatag cgaaagacgt tcaggaacga aaactgtgta tgaagtggat
    17221 agagccagtt gtataacatc ctacggcttc tttgcccgcc caaaatcggc ggattctaaa
    17281 attcctttaa gtgtggctaa caggcctttt tcgccacgcg tcgcatgccg gtcagcgcgt
    17341 cgacgacctg ttccatccgc atcccgcgcg acgccttgac caacaccacg tcgccctgtc
    17401 gcaccatccg tgccaaagcc gtggctgcgg cctgggcatc cggatgttct gtaatccgat
    17461 cagccggcat gcccgcttga cgtgcaccgg ccgccagctc ccgtcccaag acaccacagg
    17521 ccatcaggtg cccgatccct tgctcggcga gaaacgctcc gacgtcccga tgcatgcgtt
    17581 tggtatccgc ccctaactcc agcatgtcac ccagagccgc gatcgatcgt ttgtcacgcc
    17641 ccaattgcgc caggagctgg atggcggcct tcatggacgc gggattggcg ttgtaacaat
    17701 cattgatgac ctgcacacca tgcgaagaac tgatctgtga tcgcatcgcg gccggacgaa
    17761 acttcgccaa tccttcggcg atagccgcgc cggacagccc caaggcatgt cccacggcgg
    17821 ccgccgccag ggcattactc acgttgtgtt gcccctgggt tcggatacga acctccgtct
    17881 gacgcgtctt tccgggaagc accagtccga agagcgtgcc ccccttgtca tcggttcgca
    17941 cattcgcggc acgaacggct gccttggatg acgaaccaaa cgcgaccacc cggcattgag
    18001 cacgggcagc cagataatcg aaatactcat cgtcggcgtt cagcaccacc gacccgtctt
    18061 gcggcagatg atcgagcaac tcggccttgg cttgcgcgga cccttccatg ctgccgaaaa
    18121 attccagatg gtccggtccg atattggtaa tcacgccgac cgtcggtctc gcgatttcgc
    18181 acagccgggt cgtttgcccc tgctgatcga cgcccatttc gatcaccgca gcctgatgcc
    18241 gcggcgccag ctggaacaat gtctgcggca cgccgatgcg attattcaga ttgccctcgg
    18301 tcttgagcgt tttccaccgc tgtgcgacca catgagcgac catctccttc gtcgtcgtct
    18361 tcccgttgct ccccgtgatg gcgatcaccg ggatggcaaa ccggttgcga tagtgcgtcg
    18421 ccaaccgctg aaaggcttcg agcgtatccc tcacgccgag cacaatcggt gcgccggttc
    18481 gcggcttcat cggcggaagg cggtagtcct cctgcacgat cgcacaaacg gcgccttgcg
    18541 cgaagacttt cggcacgaac gtatgcgcgt cgaatcgctc tccctggaag gcgatgaaca
    18601 gatcgccttt tcgcaccaga cgagaatcgg tcaccacccg gcgaatccgc tgcttcaaat
    18661 cttgcggact caagccagtc ggcgacttgg cgctcaacac ttcacaaatt tcttcgactg
    18721 taaacaacgc cattacgcca cgatgtccat gctcccgcat gtcatgcctc gctgagcgtc
    18781 aggatcggcg cgcctgaatc atctcacgcg ccacttcccg atcgtcgaag tgatgtttcg
    18841 tcgtccccac gatctgatag tcctcgtggc cttttcccgc aatcaacacc atatcgccgg
    18901 ccttcgcctc gttgatggca gcctcgattg ctgcgcgtcg atccgcaatc atccgatatc
    18961 gaacatgact gcgagccgtc agcgcgtcct tcaccccgac ctcgacttcg ctgagaatgg
    19021 acgccgggtc ttccgtgcgc ggattgtccg atgtcaggat caccacgtca ctatactgaa
    19081 ccgcagcgcg acccatcttg ggacgcttgg tgcgatcccg gtcgccacca catccaaaga
    19141 gggtaatgat acgccccgtg cgaagcgcct gggccgcggt caataatcgg accaaggcat
    19201 cttcggtatg cgcatagtcc accaccaccg tgaagtcctg tcctgcgtca acccgttcga
    19261 atcgccccgg tacattggag acagcgccca ccgcggcacg gacttgctcc agtgttaatc
    19321 cttcgtggag cacgaccccg atcgcggcaa gcaggttcga gacgttgtgt tctccgacca
    19381 ggcgactctg aatcgggcat gtaccggccg gggtgcgcaa cgtaaaactg gtgcccgcag
    19441 gcgacaatgt cacctcgtcc gcatggatgt cggcctgttg atgcaaccca taggtccaca
    19501 ccgggaccgt gcaggcttcg cgcatccgtg caccccaggg atcatccgca ttgatgatgg
    19561 cccgcttttt tgtcttgcgc gagtcaggct ggccgagctc ggcgaacaac ttgcgcttcg
    19621 cctggaagta ccgttccata tccagatgga agtccaaatg gtcctgcgtg aggttggtaa
    19681 acacggcgac atcgaattcc gagccggcgg tgcgatcaag ggccaaggcg tgagacgaca
    19741 cttccatgac gaccgtatcc aacctcttct cgaccattcg tgcaaataat ttctgcaact
    19801 ccaacgcacc gggggtggta tgcgacgccg gaatggattc ttttccgacg aggtacgcga
    19861 ccgtgccgat gagaccgacc tgccgattcg cggcctccag catggttttc acgacatacg
    19921 tcgtcgtggt cttcccgttg gtccccgtca ctccgaccat gcggagcgca gcagaaggat
    19981 ctccataaaa tcggctgccc accattccca acgccgcccg ggaatcctgc acccggatac
    20041 atggcgtcga tccgacctga acgtggcgac ccacgacgac ggccgcagcc cccgccttca
    20101 cgacacggtc tagaaactcg tggccatcga cccgctctcc ttgcacggcc acaaagagcg
    20161 caccggactg gacgtgccgg gaatcatcgg tcaacgcgtc aatggccacg cgctgatctc
    20221 cgctccgctc cagcactcca aggcgcccct gaatgggact gatcagctca tccaacgtca
    20281 tatcaggatt ccagccccgc catcgccaac ttgaccggtt catcgactgc gacacccaga
    20341 taattgagca cctgctcgcc gacccgccgg aacaccggag ccgccaccac accaccccag
    20401 ccttcgccgc gcggttcgtc aatcaccaca atcatggcca gtcgcgggtc ttccgctgga
    20461 acatagccga gaaacgatcc gaccaggagc gtggaggagt acttgccggt tcgggggtcg
    20521 actttttgag cggtgccggt cttgccggcc acacgaaaac cggggatagc tgccttggtc
    20581 cctgttccgt gggtgaccac tccttccaga agcgtcgtca acgtccgcgc cgtgtcggca
    20641 gaaataatac gacgtttggc ttgcggcata ttctgcgcca ccaattgccc tttgccgtta
    20701 cggatctccg agacaacata cggcttcatg agaacgccgc cattggcgat cgccgaaatt
    20761 gccgtcacca tctgcaatgg agtgacaccc acttcctgcc ccatggaaat ggaggccaac
    20821 gagcgtttgc cccattggcg aggccctcgt aacaatccgg ccgtttcacc cggcagatca
    20881 atgccgctct tatctccgaa gccaaactcc ttcaaatagt cgaacacccg ccattcaccc
    20941 aacgccatcc cgaccttggc agcgccgata ttgctcgatt tttgaatcat ctgggcaaac
    21001 gtcatccagc cggctttttc atggtcatgg atgatcgtgt tggcaatcgc gaattgtccg
    21061 ttttcaccat aaatcatact gcccggggtc atgacctttt cttccagcgc cgccgctgca
    21121 atgacggcct tcatggtcga gcccggttcg taggtgtcgg tcagggcacg gttacgccat
    21181 cggtcgggag ccagggcgcc gaccgtattg ggatcgaatc gcggactcac ggccatagcc
    21241 agcacggcgc cgctccgggg atccatcaca atcatcgtgc cggacttggc actggatcgt
    21301 gtcaccgcct catccagctc cttctcggcg atatattgca ccacctcgtc gaccgtcaac
    21361 gtgaggctat gtccggcggc ggcgccttct tcattcaatc ccttggggaa aaccgcgcga
    21421 cccaaggcat ctcgctgcaa caccacggca cgtttttcgc cgcgcagata ctgctcgtat
    21481 cgcagctcca ctccttccag gccacgatca tccatcccgg caaatcccag cacatgcgac
    21541 agcagcgggc ccttcgggta gaaccgacgg ccttccatca ccacaccgac gccatcgagg
    21601 ccaagccgtt caagccgccg tccctgctct ggttccagct ttctggcaag ccacacgaaa
    21661 tgcttttcct gcttcaactt tttttcgagt tcggttgcct tcacatgcaa gatcggcgag
    21721 agattgcggg cggccactcc tggatttccg agcgaagcgg gcaccccgaa cacagacggc
    21781 acatccatgt tcatcgccag cactttgctg ttccgatcgt agatggtgcc gcgcgccccc
    21841 tccagcgtga catttttttg atgttgccgg tcagccttga cggtcaactc cgccgattgc
    21901 atcacctgaa ggttcaccag gcggacaatc acgaggataa acgcaccaga caacccgcag
    21961 gccaccagga tccggcgacc acgaaaggag ggcgaagcca ctatcgcgct ctccgggtga
    22021 ccatattctt cgcaatccga atttcgcctt ctgcgacgac aggattcgca ggcgtttccg
    22081 gctcaaagtt gatgacgacc acctggccct tctccggttg caacataccc aatttatcgc
    22141 tggccaagcg tgcaatccgt tcaggcgccg tgagacccga cagcttgact tgcaattcat
    22201 cccgctcacg ctcgagcagc actttttgcg ctttcagccg ctcgatgtgg tatccgatgc
    22261 gaacgatgtc cacccgttcc cacacgaaga gcaacaccaa cgacgtgacc gcggcaaacg
    22321 cgacggcttt cattgcatag actccttgga catccgctcc acgacacgca acttggcgct
    22381 gcgcgaacgc gggttatggt cacgctcgct ctccgacgcg atgaccggtt tttttgtgag
    22441 caccctcact gcagcgtccg ggccattcgc caaagcccga aacgtatgtt tcacaattct
    22501 gtcctcaaga gaatggaacg acacggcgca gactcgtcct ccgggggcca ctagatcgac
    22561 ggcatcgcga agcgccggtt cgatcacctc caattcccgg ttcaccgcga ttcgtagcgc
    22621 ctgaaacgtc cgcgtggcgc aatggattcg cccatgccgg tacgaagccg gcacggcatg
    22681 ctccaccacc gccgctaatt ccctcgtcgt cccgatgacc cgttgcgttc tcgcctccac
    22741 gatcgcgcgg gcgattctgc gcgaataccg ctcctcgccc aattgataga tcacatccgc
    22801 caattcacgt tcaggcatct ctcgtataag gtcggctgcc gtgcgtcctt cgctccgatc
    22861 catgcgcatg tccaatgggc catcgtcacg aaaactgaat ccgcgctccg gccgatcgag
    22921 ttgcggcgag gacacaccga gatcgaaaat cacaccatcc acatgctgga ccccgacctg
    22981 cgccacgagt gttttgagtt gcgaaaaatt tccatgccga agatgtactc ggggcatgac
    23041 gccttgcagg cgcgcgcggg attcttccag cgcatccgca tcgcgatcga tcccaatgag
    23101 aatgccatct ggagcggtgt gatgaaggat ccgggtagcg agccctgcat atccaagagt
    23161 gcagtccacg taaactccac ctggtttgca ctgcagccag aatacgattt cgtcgactaa
    23221 aaccggctca tgggaaccgc gagcagaatc catacaccca cttccaccca cttttcacca
    23281 cacgttggct ggagtataca cccaccaaaa cagctgtcaa cagattttgc tccaatttat
    23341 caagaatatt gttcgctttt gacccatcgt ttgcaggccg caatttggca gcaaagttat
    23401 ccacatttgt ggacaaacct gtgggaaact atcccaccct tcgattaatt gcggcatgaa
    23461 aatggttgtg aaacgcgaga cccgtccaaa aaatcagaca gatggaatgg cagcatttcg
    23521 gtgagcgcag gaggaatcag ccgatgggaa ggatggggaa aacctatgga tataaccggt
    23581 acaacattct ggggaaagga atcgtctccc gcacatgttc gaggccgcaa atccacgcca
    23641 ccgcgcgctc gattcccatt ccgaatccgg catgtggaac cgagccgtag cgacgcagat
    23701 cgagatacca ttgaaacgct tcgaccggga gatggtgctc gcgtatccgc gccaacaatt
    23761 tctcgtgctg gtgaatgcgt tgcccgccgc cgatgatttc gccatacccc tcgggcgcca
    23821 acacatccat gcacagggca agatcagggc gagccggatc ggtctccata tagaaggctt
    23881 tgagcgcagc cggatatcga tgcacgatga cggggcggtc gaattctttc gataaaatgg
    23941 tttcatcgtc cgcgccaaaa tcatctcctc gtttggcggg atttcctttc cgctgaagaa
    24001 tgtccacggc ttcctcatac gtaatgcggg ggaacggcgc cgtcacgcgc tccagcttcg
    24061 cgatatcccg ctccaagatc tgcaattcgg cacgacgcgt cttcagcaca gccgcgacga
    24121 tatgcgccaa aaactgctcg gcgagatcca tcatgtcggg cagttcggcg aaagccactt
    24181 cgggctccac catccagaat tccatcagat gccggcgcgt cttggacttc tccgcgcgaa
    24241 atgtcggacc aaaacaatag acctttccaa aggcggcggc agtggcttcg ctatagagct
    24301 ggccgctctg cgtcaggtac gccgtctcat caaaatattg cgtctgaaac agtgtcgtag
    24361 tgccctcgca ggcattcggc gtaaaaatcg gcgcatcgac cagtgtgaac ccgcgatcat
    24421 cgaaaaaatt ccggcaggcg cgaataatct cgtgacgaat gcgcagcacg gcatgctggc
    24481 gactggagcg cagccagaga tgccgatgtt ccattaaaaa tcctgtaccg tgctctttgg
    24541 gctgaatggg aaatggctcc gccacctgca agacagcgag gcgcttcaca tcaagctcgt
    24601 acccgcccgg tgcgcgcgca tcctgccgca acgtgccggt cagcaccacg ctcgactcct
    24661 gcgtgagctc ggctgaatgg gcaaactgct cgtctccgac tgcgcctttg ctcacgactg
    24721 cctgtatatc gcccgtcccg tcccgcacaa tgagaaagtg aagttttccc ttgtcgcgtc
    24781 gactgcggag ccatccgcga atcgtgactt cctgacctgc atgggtcgca gcttcgtcga
    24841 tatagatcac gggcatgggc attccccctg gctatgaacg tgagagactt cgattatgcg
    24901 ggagggtaac gaacggcctc ggccttttca agcgcatcac aattctcccc tttgattgac
    24961 acaatgtctc tcggggggca tcatcaaaac catgatacag acaagtacgc tacaccctcg
    25021 gcattggctc gcgtcagcgc tcatcgggct cctgctggtg ggactcctgc ctgcggttca
    25081 ggcgggagaa ttcaagtcag acaatccgct caatcagccc ggaaaaatct attggtgtcc
    25141 gaatcgcgct gccgacaaac gcattaccgc cacccctgat gcgggttgca ctgcgctgta
    25201 cgatgcagag cgggatgaga aaaagcccgc cgcactcaac caagacgagc gcaacgatgc
    25261 cgcaacaaaa gaaccgctca agatcctcga tattcaaaat gaagcctcga agttcacccg
    25321 cgactaccgt gaatttatcg attgctgcgc gagcgaatgg gattccctcg atcgcatcga
    25381 cgaacttctc gaacagtcca atcacatttt gatgtcggtt caacaaaaag gcatctataa
    25441 cagcgcgggg ttcggcgtgg gaagcggcgc gagcggtttg ggcggcggcc ctggccagtc
    25501 tcccaaactc ggcacctttg cccggcaatg gacgctcagc gaaattgtcg gcactgtcgc
    25561 catcgcgaga gatgacctca aaaaactcag gactcggatg aagggcctca tcacgcgata
    25621 tgaaaaactc gactcacttg attacgaaca gcgcgggaga gaagaacgac gcattgaaga
    25681 tgaacgcgag gcgattacgc acgatcttcg accaaaacga ccggcaacct ccgcgccaac
    25741 cggcatggac atccaggaca ccaccatccc gaccaggatc ggcggcgaca tcgagaacac
    25801 gcatttaaac cgttccctca atccgagctt cggcgcggac atcggcacca cggtctcgcc
    25861 ctacagcaat gtgaacgaat cgctccgtcc acgcagaggg gaagatctta gggatagcgt
    25921 gttaacggat cgtgtcggta ctcagacgca agacacgacc ttgccgaatg catcaggctt
    25981 tgagatcaat gcggccgaga atgccgagca gtcctcctct gcgcacattc gaggagtcgg
    26041 tccatccatc ggtgactctt ccttgaacag ccaatcgcgc cgatagcctc gctttccaac
    26101 cgcctccagc ttagcgacac cacctactag tcctttggaa ataccgacgg catcgcctgc
    26161 caatgggcgc cgccatccac actcttgtag agcccgctgc cgttcgtgcc cgcgaagagc
    26221 acgtgtggat ccatggcact ctgggccaat gaacggatgt tggtggttgt caggccttca
    26281 ttcagcacta tccaggtctt gccgccatcg ccgctccggt gaataccatc ccgcccggcc
    26341 agatacatta cgccggcctg cgcatgatcc agcaccattg ccacaatcat ctgatcctgt
    26401 aatgactcac cgatgcgcac ccaggacact gcgccatccg tggttttata caggccggcc
    26461 agcgtcgccg cgtagaccgt gtcgggcgcg aaaggatcga cttgcaccga cgtcacaccg
    26521 agcgcacgcg acgtcttcac cataccttcc ggcaccaacc cgttgttcac ctgtagccaa
    26581 tgcgcccctt ggtcattcgt cttgtacacg cctccactgg ttcccgcata cattacggag
    26641 ggatgcgcgg ggtccatgcc tagcgtcacg accatcaaca cttccttcat gccctccatc
    26701 ctcttgaccc accgctcccc ggcattcttg gattcgaaca cacccatcgt cgtcgccagg
    26761 aagatgtggt tatcgtctga gggatcgaag acaaattgat tcacgacgga cgatatcgtc
    26821 gcatcatcca atccggtccg gaccgaggtc cagcgctggc cgccgtcata acttttgtag
    26881 acggcatccc ccttcgtgcc cgcatagatt gtggcgggat agacgggatc aaccgccatc
    26941 gcaatcacac gcgagtaact catccccttc gagagattgc tccacgtctt gccgccgtca
    27001 cgcgatttgt aaatgtagtc gttggtggcg atgtacagaa tgtcggggtt ggtgggatgg
    27061 agttggatga caacgaccgg gtcactgcca ccgcatccgc cgagcaagag accggcaagc
    27121 aggaacatag aggcaagaaa tttggtcatg cttgaaccgc ggatccacag gggatggtca
    27181 aacaggtcgc tcagcgaggc cgcagcgagg gaagaggcga ggcgtactgt gcttgtacgt
    27241 tgagcctctg atcgaagcga gaacgaagct gggcacctgt ttcaccatcc ccaggctagc
    27301 ggtagttggt gaactgaaga tcaatcccaa aatctttccc cttgagaaag gcgatcgccg
    27361 actgcagatc gtctttgctc ttgctgatca cccgcacctg ttcaccctga atttgcgcct
    27421 gcaccttcaa cttggattct ttgaccgcct tggtaatttc cttcgccttt tctgaggaaa
    27481 tgccgctttg aatcttgatc acttgacgaa cggtcgcccc gagcgcctcc tcgatcttgc
    27541 cgtactcgaa ggccttcaag gagacgccgc gcttcacgca cttgctcttg atgatttcaa
    27601 tgacggcatt gagtttgtac tcatcgtcgg agagcacgac gagttctttt tctttttcct
    27661 ttagcgtcag ctcggtcttg gaatccttga agtcgaatcg ctgcttgatc tctttcgtcg
    27721 cctgatcgac gacgtttttc atttcctgca tattcacttc cgacaccaca tcgaatgatg
    27781 attggtcagc cacgatgtcc tccttctacg tctctctatc ctaacaacaa ccgccgccat
    27841 ggggcagcgt gaatttgcgg tcatcatgcc ggtgaatggc cgtctgcgca cggcctgcgg
    27901 atccgccgcc ggcggtcgcg atcacttcac tgctgctgaa cgggcgtttg agaatgacat
    27961 acccatacca ctgcttcatg ctatcgctca agacgaccac gcctagaata gccaccacgg
    28021 ccaccagcac ggcatcgaga tagagcgcga acgcctgccc cgacgccagc gtcgcggcct
    28081 tcgccagaaa catccagaac atctcatagg agccggccag cgtaatgcct cccacgaaca
    28141 acatagggat ggccgtcacc cagagatagg gcgacttcca cattttgatc aacaccgtcg
    28201 tggcgataca gagggccagc gtgcccagca actggttcgc cgcaccgaac atcggccaga
    28261 tggtggagat gctgccggtc ccgatcagat aggcccagga tcccaccaca aaagcactgc
    28321 tcagcaacac gccgggccac caattcattt gccgaaaagg cgcgtacaca cgcccggcca
    28381 tttcctgaat cagataccgc gcgacacgtg ttccggtatc aatcgtcgtc aggatgaaaa
    28441 gcgcctcgaa gaccagcgcg aactgatacc aataggccat caagccggcc atgcccggca
    28501 acgccgaaaa gatggaggcc atgcccacag ccagcgatac ggccccgccc ggccggcccg
    28561 ccacattcac ctcgaccaac tgcgacaatt ccgctacgcg cgatggcgca aaccccatcg
    28621 ccgtcagcgt ctctgcagag agcatcgtgt tgatcgccag gtaatcaccg gggatcaaca
    28681 ccgaggccgc gatcaatgcc atcaccccga cgaaactttc cagcaacatc gccgcatatc
    28741 cgaccaccgc ctgcgactcc tgttcgatca tcttgggagt cgttcccgaa gacaccagcg
    28801 aatggaagcc cgatacggcg ccacacgcga tcgtaatgaa caaaaaagga aacagcgtcc
    28861 cgggaataat gggtccccca ccattggcaa atgcggtcag gcgcggcatc tcaatggtcg
    28921 gcgccatgag aatcacgcca agcccgagca gaaacaccac acccagtttc atgaaggtcg
    28981 agagatagcc tcgcggcacc agcagcatcc accccggcaa gaccgaggct acgaatccat
    29041 acccggccaa tagccagacc agcgccggct tctcgaattc aaacaaccag gcatagggcg
    29101 attgtccgac gacgcggccg aataacactg ccgtcacgag caacaccagg ccgagcaccg
    29161 acacttccgc gacggcgccc gggcgaaatt tctggagata aaatcccatg atgaacccga
    29221 tgggaatcgt catggcgatg gtaaaggtgc cccacgcgtt atggtagagc gcattcacga
    29281 ccgcaaaccc cagccccgcc agcgcaacca ccacaataaa caggaccgcc accgccgtcg
    29341 cggtgccggt tatggagccc agttcatcat gcgcgatttc cggtaacgag cggccgttgc
    29401 gccgcatcga ggcgaccagg ataataaaat cctgcacggc cccggccaga accgcaccga
    29461 tcaccagcca gagaaagccg gggagaaaac cgaattgcgc cgccagcaca ggtcccagca
    29521 aggggcctgc ccctgcaatg gccgcgaagt gatgaccgaa caggacgact ctgttggtgg
    29581 ggtgaaaatt caccccgtcg ttcaaccgca cggccggcgt tagacgccta tcatccaatt
    29641 gcaccacttt ccgggccaac caccggccat agaaccgata ggccaggaca tagatgcagg
    29701 ccgccgccac caccagccac agaccgttca ctttttcggt ggggttgatg acaccgacca
    29761 catgagccag cgccacagct cccagaatcg acagcagcat ccagagcaca attaacgcag
    29821 ttttcatgcg cgggatagta gcacaaacac aaggagatgg ggcgacgtga ggccgtacgt
    29881 gcgggggccg gaccatgtag gggaacagga agtgcgggac ggttgagcga gaacctgttc
    29941 accgggtttg ttatactttt tctgaaccat gtcgcctgcc acgccttcag agccatccgg
    30001 gcgagagcgc cgcgagtttt atcgcatcac cgtcctgctg cccatgtgcc ttcagatggc
    30061 aggagacgag accgaaggtg agttgattga gacatccgtc aacctcagtg ccggcggcat
    30121 cggggtgatg gtcaccacac cctacaaatc cggtgacgtg ctctctctta cgcttctcct
    30181 cccagatcag gttcgcttta aagcgtcaat cgatgtgtta cggcttgatc cgcttccgct
    30241 cctcggcggg gtatatcgcc tccacgctcg cttcgtcggg ctgccggctc acgaccggga
    30301 actcctcgtc agatacattc ttcgttttca gcgcgaccat ctgcaggaac actattccgc
    30361 ctgactgtcc caatgccctc actcgcgcgg tactcagaca tccacggaca ttgatggatg
    30421 aactttcccc gtttgcttcc gtgacctgta acaggggatg atgcccgcat gcgcatgctc
    30481 acgtggcttc tcgggacaat cgttgcactc gccactatcg gtgccgtgta tcagattgcc
    30541 ggcatggtgc tggatcggca gcattatcct ccacccggcc aactggtcga tattggggga
    30601 catcggctgc acctcttttg tcttgggacc gggagtccga cggtactcct cgaggcgcct
    30661 gcacctggct ggtcgcttta ttggagcacc gtgcaaccgc agctcgcccg aacgactcgc
    30721 gtctgcgcct atgatcgagc gggcctcggc tggagcgagc gcggtccctt gcctcgcacg
    30781 ggacaacgaa tggcgaaaga actccatcgt ctgctcgaac gagccggcat ccgggggcct
    30841 tatattctgg taggacattc gctcggcggc ctgatcgcca ggctctacca acatgactat
    30901 ccccaagaag tcgtcggcat ggtgctggtg gatgcgggtc atgaatcgga aatgcgtcag
    30961 gcagaatttc gattgttcgc caacgccggc aaatccatgc tgcccgtcat tcgcgccctg
    31021 acaatggtag gcatcacaag gctgatggcc tcctatgacc agctgccgcc gctcttgatg
    31081 agacaggaag agaaggtatc ggccgagact cgcccgatgc tgcgcgccgg ctggctacgg
    31141 accggctact attccacgct gacggatgag agtgacggat tgattgaaac gctggagcag
    31201 gtgcgccgca gcggttcgct cggcaatctc ccgttgatcg tgatcaccgc cactggccca
    31261 gtctggtggc cggacatgcc gggacaggtg aaccaggcca agttcaggaa aatgtggtta
    31321 gaactgcaac agggactgac caccctgtcg acaaacagcc ggcaggtatt cgccgaccag
    31381 agcagccact ttattccttt tgaccagccg gaacttctca ccgatgtcgt tcgtcaactg
    31441 atcgaccggc agcgcagcgc cccgtaccgt ccctgagggg ctcttcccca gcagatcagc
    31501 gagattcccg gggaagactt cattacgcct cttgtccaag gcatacagcg tggcctgtga
    31561 ataatcacgc agccttaggc agctggacgt tatccctata tgctctcatc ggaaccgccg
    31621 gctgctggga ggtagacacg gtgctggact cagaagacag catccagagt cccatcaaga
    31681 gcaccgcgat cgcacccgcc accgttagtc cggcatcaag aaccgaactc aaatccatca
    31741 tcgtgttcac ctcctttctg ttccagatag tcatcttggg atcacaaagc aggctctcag
    31801 ggcgcccaca cttaaaatag gccgtgcgac cacggggtca cgggacgatc ctgatgatct
    31861 tctcttcgct cctgccgctc ctgggcccgg cgcctccgcc accacccgcg tagggaggcc
    31921 agtgacatcg cgccctgcaa cccatgcgta cccaattccg agcggctacc tatgcaccgc
    31981 caacgcttca agaaatttct gtagttcata ggtcttctcc ttgagcattt cacaatgccg
    32041 cacatctctt ctgcggcagg tcaaactacc tagccatgat ggcttccggc gtgagagtag
    32101 aggcctcgcc gatcgtggga atcagcgagg cctccgggaa caaccggtta ttcggaggaa
    32161 ggcgggaggg gaaccgtctg aggatccctt cgcttcatca tgccttctgc gcaagacgga
    32221 tgggagtgtt taggtccctt caatcataat ccgccgactc tttgcttctt cccgcttggg
    32281 aaacgtcacg gtcagcaagc cattcttttg cacagccgaa gccttctcgg aatcaatata
    32341 cgtcggcaat cggaagagcc gcataaaccg cccgtcaccg atctcttgca catgaacggt
    32401 cttacccggt tgaggctgtt ccttgcgctc gcccttgatc gagagtacat gcttagccac
    32461 ttcgagcgag acgtcttgcg gctcccaacc ggggagcgcg acctgtacgt agaacccgtt
    32521 gtcatcttcc caagcattac tggccggagc ccaggctatt ccgcttacgc cgaacgcgct
    32581 taccgcatcc tccagtaacc gatcgatttg ccggtcgaag gagtcttgcc cgataggtgc
    32641 taccaatgct gcatgataac tggtcatacc gtcctcctcc tctctcctga gaggtcagtg
    32701 taatggttga tgtgagtggg tttggatgga atgctggccg aagcagaagc ggggcattca
    32761 cctgccgcca ttgccctcca gaccgggcac tgtgtcccgt ccatccggtt acgcattagc
    32821 aacgatgaaa aaataatccc gtctcccatg aagtcaaggg ggcaatttca cggctacaga
    32881 agaagggaaa aggaccgagg gggatacggt cgcgctagga acgcaccggt tcgtagatct
    32941 gatagcgtcc gatggtcgtc acgagccgca cacgattttg cgcaatcaac ggcttgtaga
    33001 atcctgccca tcggccatat tcgccgacta cgagatagtc cgggttctgt tgcagagggt
    33061 catacgtgac tgcctgcgcc tgtccctgtt cacgccggat aatctcaacc agatgcttgg
    33121 gcaaggcgta ggtgtaggga cgattcaaaa agaggaacaa ctcgctgtca taggtttcga
    33181 tcaacgccgt cggtgaggtc gtcgtgttca gatgctgcgt caccgcaaac aggtcgcgat
    33241 cgtcctctct cgctcgaaag gcataccgtt cctgcacggc gccccacccc atgatcgcca
    33301 ggagcagaac cacgacggcg gccttgaatc cctgaggggt cacccgccga cccggcacga
    33361 gttcagccag agtccttaac agataccgca catcgaatcc tctggtggcc tcgcaaacaa
    33421 acaaggctgc aaacggcgcc gaaagaaata atccgggcaa agcgtaacgc ggttctccgg
    33481 cgctcagaaa cgcaaaccag gccagccaac tccctgccag cagcaacagc atgatctgcg
    33541 ccgtctgtgc cacggactcc tcatcgcggg aagcaggcgt tcgatgaagc cgccaggcgg
    33601 cataggccag tcccaacgga tattcagccc atgccacgaa caggtactgg atcgtctcca
    33661 ggcggatggc cggcacgaat accaaggcaa tggcttctgt cattccatcg gtggagggat
    33721 gcgccatcgt gtgcccgctt aacagccagt tcttggcttc gatcaagaac cgaaatccgg
    33781 cccatgaacc gatcaaaaca agggccagtt ggcgcacagc cgaccagttc ctgcgaagag
    33841 tgagaatgac gatggtcccc acgaaagagg cgatcagaaa tggggccacc tgcgccttag
    33901 tcatgaaggc caacgaccag cacccaattg cagcgaccag ccagagaggc cgatgcgcgc
    33961 ttcggagaaa gcagacgaag gcagccaaca gaaaacagag taacggcatc tcgcccagca
    34021 cctggcgccc cagatacagg ggatggatcg accatttcag cggcacgagg atcagcagtg
    34081 ccagggcggc aattgcgacc gatcgtccaa agaggcgacg agctaccgca tagagcagca
    34141 gcaacgccgc gaaggtgtac agcagcccca ccaccctcgt ctgccacact ccgacaccga
    34201 acagggcaaa actcgccgcg accgacgcca cgacagggaa atgcgccgcc aatgaaggcg
    34261 gagccggttc tccccggagc agacagccgt agtgacctga ctcgacccat gtccgcgcga
    34321 cacagacggt ccacccttcg tcaaaaaaca gtggagggaa actctggata tggacgaccc
    34381 cgctgccgaa gaccattaga aacaaacatg cagccaccac gaggtctgtg ccccacaaag
    34441 cctcattgcg ctggccgatt ggagacggat cagtcaatcg cactcgggta tccccagacc
    34501 cggttgttcc ggaaggcgca tcatcgtcgt cgtgggggag caatggatga ggtgaggtcg
    34561 ttggcatagc agatgacaat tcacccataa accaatcgtc gaatcattgc aggacaggac
    34621 accctccggt cctcgccttc aggccgaagt cagtcgcaca gtggcacgtg gatccggcac
    34681 gccaatctcc tgctccagat ccaatgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34801 nnnnnnnggc cacatgccgc ggtcctgggc gaactgccag ctggtatcga acggatacat
    34861 atctgccacg acaatatccg cctcagacac taccggcacg gcatacatcc gctgtgccgc
    34921 acgaacccct tcctcatggg ccttgaccac atcaccggca aacaagccgc taatttcgcg
    34981 acggtggttg agcgtgacat tggcaataaa atcgaggccg gccttcctgg ccactgccac
    35041 catatcctgc cgcagctctg tatggatcga accgcctcgt gtgcgtgaac cacgcaagta
    35101 gtcgtgcatc atcctcgtgg tctccacacc gcagacggca gggagcacaa tttttgcgcc
    35161 gcccgagaac ccggcgatcg gatgaggata gatgcacccc actccgatct tcagatcaca
    35221 ttccatgacg gtgcggttga tccgaatcgg caacccgccg ggcgacatgc ctaaatccac
    35281 gagggctccc cggctgtcgt gcgccatgac tcgcacggcc ggcgagaggt tggcgccgac
    35341 ctttttctct atttcctgcg cggaggcggg aggatgagtg ccacctgcca agagaatggt
    35401 gatcgcgtgc tcacttaatc cagctgcttg gatctctgcg ataatcgacg ggaggaggtc
    35461 tgccgcagga gtcggccgcg tgagatcgtc gacgatgatc acgcatttgg tctttccctt
    35521 ggcgatctca cggagtggtg gagaaccgat gggtacattc aatttttcgc gcatggccgt
    35581 ggcactcaat gccggggcgt cctgagggcc gacctccaca atattccacg caggcggaaa
    35641 attgatcgat agcggctcat caccgaacca cgccttcgtg cgtaactgca cgtcgctcat
    35701 gatttgctct ccaccctgga agaaaccgct tgcaccatag gtttcgtctt acggtcaatg
    35761 tgagtcatgg accttttccg gcacatctcc cagaaaaacc cggcggcctg acatttttct
    35821 cccagccaaa agagacaaaa atgtcgttta cgctgaaact tctgccattc gaacggcccc
    35881 aacattttta cccagatggc cttcagccaa cgcggcttgg ttttctttcc accctcgccc
    35941 cacaacagac tgtcctgcgg caccactccg ctggaatccc tacgcatgac ccggaataaa
    36001 atcgcaattc ccgcgcctcg atgaaaggaa tgtcgcagat gttgcagtcc tgtcgcccgc
    36061 cgcagatggg tcacgggatt gggaacaaac accatcttgg cgccccgctt gcgcagccgc
    36121 caaatcaatt cccaatcctc gcctgcgccg gtgaagagcg gattgaagac atacccatct
    36181 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncccgtaga ccgggcaatg
    36301 ccctcgtttc gtccccacgc gaccccgcgg cgatcgcggt cgagaacaac gatgacttca
    36361 tccggcaacc gagtctgttt cgaaagcccc tcctggcaca acgcgagcgt ctcccgcccg
    36421 agagtaggaa tcacgacaga gatcttgtgc tgatcagtat ggagtttcag cgttccccct
    36481 cctagttgga tgccgcagct ttcaaccgca ataacaacgg agccaccatt cgaagcacag
    36541 actctcctgc cttccgaaac gtgcccggca gactctgcac gagcgattcc agttcgtcca
    36601 cctgcacgat gccaaggctg aagacgcccc cccaatacag aaagactcca acggcaatgc
    36661 ccaccagaag agacagcatc ggcgtagcga cgagtgcgga gagcccggcc attgcgatac
    36721 cggtcatgac gacaagcccc actaatcgcg acagcgctag cccctcgaag acgctgacgt
    36781 tccgccgccg taatatccag agatagccgc ccgccaccag cgcaaaggag agggacgtcg
    36841 cacaagctgc gccggctgca ccaccggcgg gaatcaggat cgcgcagagt ccggcatcca
    36901 ccatgaccgt caggagcatg acccggatca gggacattgc ctcaccgccg gcttgaatga
    36961 ccggccacag cactcgggcc aaggcataac acaacgcgcc gggcaccagc agacgcaacc
    37021 ccagggcagc ctctgaaaat tgggagccaa agtacaccgt gataatccgg tcgcccaaca
    37081 caaacaccac gatcagcaga aagaccgtgc ccagcatgac gtagcgtacg agacgactca
    37141 ccaggctggt cacagcctct acccgctgtt cagcccacag ccgagcggtc gcttgcagca
    37201 tcactccttc gaccgcaatg ggaatgaacc agacgaattg ggaccactgc accgccgccg
    37261 cgtagaggcc cgcctgagct tccccagcca aatgtcggac gagaatgatg tccagtgagc
    37321 agagcgccat gttgagcacg gaaaaaatca tggccgacaa tccgaatcga agcacggctc
    37381 ccgtcggcaa agcgggaggt ccctgagacg ccggaagccc gaggccgttc aatgccacca
    37441 tcaccgctct cagacaggcg cctgccacaa agatatccgc caccagcaga ccgaccaaga
    37501 cgccaaccac gcccatcccg gtgaccgccg cgataatgcc gaaacttcct gccatcagga
    37561 cgcccatgat gccgggcacc gccgcgcgtt cttcatgtcg taaaccgtgc aacacgccgc
    37621 gcgcataaaa ccagacctgg tcaaatacga ggacgcccac cacggccaac gcaatcggca
    37681 cgcgcgctgt ctcgtgcgac gccgtgcccc agatcaacac tccggccgca agcccgacaa
    37741 atacgactcc cgtgccattg atgagcgcgc cggagcgcgc gaccccccgg caccatgctg
    37801 catcgtcagg ccgctcggcg atgcgcctgg tcatcgtttg catcatgccc acattggcaa
    37861 attgcgaggc gagcaagagc accgccaggt aataagcgta ctgaccatat ccttcgatgc
    37921 cgagaaatcg cgcgagaata ggcaggacga cggctgacgt ggccagcttg gccatcgacc
    37981 acccgccgac ggagagaatt cctttagaaa ccgctttcgc cgacatggct cttcaacatc
    38041 gaacactccg ctgttccgaa gtccgtatac acgcccttca cagacgctta tgccgcaaaa
    38101 cgcgcatatt gctcttgatg ggcaatcaac gctctggagg tttcgtaggt gtcccgcaca
    38161 tagtgatagg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcttgacc
    38281 ttctccttga gaaaatgggc cggacaatac accgcatcgg cccgccgcac cgcttgcctg
    38341 acgagaaacc ctgattctgt gaaatagttc aggggcgtca gcagacggcg catcggagtg
    38401 gcatacagaa actccaccca ccagtcgctc aattcccgcg gatcccggct cgtcaccaga
    38461 tgcgcgcggc gcgggtgaat gcgttgcgcc aggtaggtca ggaccgtagg atcctgggaa
    38521 tgaaaaatgt ccgcgcggga ttcgcgaatg aggcgacagg catccatgac attgcgcggc
    38581 gagaagctgc gaatctccac cccgccgatg gtttctgtgg gctgttggcc ctgacgaccg
    38641 ggcacgatca cgctgacccg atggccggca cgcaccaggc tttcggccag cttccgcgac
    38701 atgctgccga agcctccata aattccccaa tcaaaatact gactggtcag aatgcacaca
    38761 tgaggcattt tcctcatccc cggagctccc tggtccaatc gcgagccacg tttttcgcac
    38821 ttcgggcatc cttggctcgg ctttcggcgg acggaaccat aaccctcgcg cacaactcaa
    38881 ggtagcgggc ggcgagcgtg ggccatccga atgcgagcgc acgacgacga ctttcgtcgc
    38941 ccatgcgccg gcgctgctca gcagcgttca gcagccgctg caacgcggtc gtgagtgcct
    39001 caacatcatc ccatggcacg atctcaccgt tggctccctg cgtcaccaat tccgcagtgc
    39061 ctcccgtatc ggtcacgatc accgggagcc cggaggccat cgcttccaag agggcgatcg
    39121 acatgccttc atgctgagac ggcaacacga acacatcggc gtcgtgatag acaggaggca
    39181 tgttctcgcg cggcacaaag cccttgaacg tcaccgcctc cgccacttgt aacttcgccg
    39241 ccaattctcg caactgcggc tcggcatccc cggtcccgac gaagatcagt gccatcggtt
    39301 catggtttgc tgctcgcaag ttggcgaagg cctgtagcag atgatgttgc cctttgcgct
    39361 caatcaatcg agcgacacaa atcatggtga acggccgtct cgaatttctc gtggccggga
    39421 caaacgcgtt cgtatcgacc ccgttgggaa tcgtgataag gggcagatcc ggtatcgtgc
    39481 ggtgcgccaa cgccacctgt tccgcgctga tggctgtcac cacacccgct cgacgccaga
    39541 tctgtgtgat gaacggcgtc aacaccgggt agaggtagtt gtatcgcgcc tcgaaccccg
    39601 gcacatcggg cccctgtaac gacaagacgt agggcagtcc atgcgtgaga cgcaagaggt
    39661 aactgatggc accggccggc acacctgcaa aggcgaagct cacatcatac tgatgttcgg
    39721 acgctagttg ccgtgcgaga cgcaatcccc gccaactgta ccgcagcaat tcacgattgg
    39781 tggcgtggtg gatgttgcga ttatcgaccg gcaccttgta gatcgtaatg cgatcggcaa
    39841 aggcttcggt ttcgtatcgc gccttggttc gcgaggacgt cacgagatcc accgtgacat
    39901 cgggccgcaa ggccatctcc tccagcaaat ggagattgac gacgccggtg ccgcccccga
    39961 ggggagggaa ctcgttatcg aacatgagaa tgcgaatggc gattatctcc tggtcacgcg
    40021 gcgggcgacg tagagcggcc ggtgtttcac ttcatcggat atgcgtccga tgtattcccc
    40081 gatgacgccg atcgtcatga gctgtatccc cgcgaggaag aaaatcgaga cgaccagcgt
    40141 ggtgaacccg gccaccccga ccccgatggt gagtttcttg atcagataaa acagcgccac
    40201 gaggaaggac agcagcgaga cggtaaagcc cagcagcgtg atgatgcgca acggcatgtg
    40261 gctgaatgag accaacccgt cgagcgccag atagatcagc ttccgaagcg tgtacttggg
    40321 cgtcccggca tggcgcgcct ggcgctcata cggnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40441 nnnnnnnnnn nnnaaaaccg ttacctggga ttggatatct cagggttgct ccctagtgta
    40501 gccgaggagg tccaattcgg ccacatcttc cactgaaatt gcgctgtagg aaattcggca
    40561 tggctggtct gtctcagcct ggcacaagtc ccggtgtgga acggaggatt atttttgtga
    40621 aggggaagga tcggcacctt tcgtcatcat cgccaggcag cccagcccta aggcaatcca
    40681 cacgaattcc cgaaagcgcc gcagaagcgc aaacgtaata cctgtgactt cgccgtaccc
    40741 gaacgcggag aggaggaaca gattgcccgc atcctgtgcc cccaaactgc ctggaataaa
    40801 aaacgtccct cctttaatga aaacggataa ggcaccaatg gcgacagccg gcaacacgtt
    40861 aatcgattgc cccatgcaca gcaccatgac gtagacttcc agcgcctccg cgagccaacc
    40921 gagcagaaac agtccggtcg aaaggagaaa ggcctgtcgt tggtgggaat aaaagccggc
    40981 gatggtgcga tccagttcga gcaattgctg ctcacgcgcc tctaaaaact ggatgcgcag
    41041 accgatgcgt cgcaacagcg ccaggatcca cccgaacata ccctgacgct gcacgatgac
    41101 aaaggcgcca acaccgaaga gcagcaggcc cacgctgacc aatcccgcgg taatcgtctg
    41161 tccggccgat ccgccggaac ccaatagcca gaatcccagg ccgatgcccg caagaatgaa
    41221 cagaatctgg gcgatggtca tggtcgtttt tgccgtcacg accgacgcga ggccctctac
    41281 gagtggcacg ccgtggcgat tgaggaggta ggccttcagc ggttcgcccc ccacataggc
    41341 cgtaggcgta gtcatgttca cgacttcgcc ggcggtacga atcgctaaga tgcgccaaaa
    41401 cggcacaccc ttcgcccagg cgccgagcgt cacgcgccaa ccataggctt cgagcacata
    41461 catgaggaga gagggaagaa ggaccagcac catggcggcg ggaccgagtt gcgtcaccgc
    41521 atcgtaaata cgcccgatgc cgatatgcca aaccagtgcc gagagtgtca gcgccccgag
    41581 gagaaggagg gccgctttaa gcacgggccc gtgccgaggg tcggatcacc cacaccatca
    41641 tcaaccagaa aacggtcgac ccgagtgcag ccatccagag aaaccagtcc agtttatccg
    41701 cgacggcaaa caggaagacg accacagaga agtcgcggct cgccacattt ttgagaatga
    41761 agtccgacca ggcagcctgc ttcggggtat tccagcctcg cgtagcaccg atcttttggg
    41821 ccttcgtgac cagccagaac gccatggcat taccgaacac ggccgcactt ccgagcgcca
    41881 atgacaccca cgcctccggc gcatcggcgt gccgaaggta ggccccgcag gcaatccctg
    41941 caaagatggc gatatgcacc acattgtcca tggcgatatc cagccaggca ccgaacgggg
    42001 attccgtaaa agtgaggcgc gccacttcac cgtcgcaaca gtcgatgatc gcagccagct
    42061 gaaacaacag cgctgcaacg atccctgccg tataggtccc caacccgaat ccgacagcgg
    42121 agaggagccc gacagcggtc gccaccattg tgatggtgtt cggggagcat tttaattgga
    42181 gaaagagtcg cgttaagagc cgcgaaaatg tccggttgaa ataccgatcg acaaaaccct
    42241 cggcttcgcc tttcagcgaa cggaacagtg tgcgctcagc gtgtccggca ctggcctgat
    42301 cccacacttc gcgataccag agtccggcgt gggacgcggc agccacgacc cgcacccgtc
    42361 cttcgatggc ggcccgttcc agccagcgac gcatgggggt catgccgggc gcttccgcga
    42421 ggccgctcgt caacggggca gccgccccgc tgggcgggtt caaaatgctg gcgggcaaca
    42481 cgaccaggtc ggccgccacc tgatgtccct gttgcacggg ctgattgtga aatgaaacca
    42541 gccgcccttc ctgaaatgcg acgaccggat tcctgcgacc ggccgcaggt tcgatcgatc
    42601 ccgcttcacg cgtgaccacc agtgcctcgc cgtcgcgcac ggtctgccgt aaatgttcaa
    42661 tgaggccctt ggaaaacacg gcctgaactc cggccacgag acagaacccc cggacttcag
    42721 cggcgaggga ttcccaggta cgcgggtcgt cgagcggaaa ttcacgcacc ggcatccacc
    42781 ggaccggaat cgtcacgcgc gcgcctcgtg ccagcgcttg cttgagcagc tcttcatcgg
    42841 gaccggccaa cacgatcaat tggcggatgc cgccccgttg caaggtgaga accgtccgct
    42901 gaaagaggct caaccctccg atgtgagtca agggccccac gtcggcaaga cctcgccccg
    42961 gacgatcacc gaagagaccg gcaccaggca gtaaaatggc cgtgctcaat ccctgcagct
    43021 ccgcgcctcg ttgtaaggta ctttcactca tgattccgaa tggggttcct attggtggat
    43081 ggccgctggc gggtgcctgt gcgctacagt ctcgggagaa tttcccgctc cgccttcgtc
    43141 acatcttcag gaaaatcgat ctcggtccag ggcagtcccc cgattttttc atgcccgacc
    43201 ttcacgtcac gaaaatactg caacaatccg tcctcgtatt ccatgtccca ccggtcttga
    43261 tcgatgtacc ccttgaggga agcgatcaca tgcgctgtat ccgcccggcg cacccggagg
    43321 aacccgacac cttcgccggc gagatcatac cgatcaggca ccttcttgct caaggcgatg
    43381 acgcggtcac cctgcaccac caccatacat tcctcgcccg tttgcttcac cgtatcgtcc
    43441 atgagtaaac agttttcata gggggacgcc acaagacgcc gaaggatctc ccgatgaaac
    43501 aacacatccg cgtccatgat gaccacatca tcggtcagcg cctgcctggc aatccataac
    43561 gaggaaatgc tgccgcgatg gaactcctcg ttgacgaggt aggagatatc gacgccatgg
    43621 cactgtgaac ctaccgccgc cctgatcatc tcctgtttgt agccgacgac aatggtggcc
    43681 tgcctgatct tcaccgacgc cagggattcc agatagcggg acaacagggt ttgcccgccg
    43741 atctcgatca ggcatttcgg cttatgctgg gtcaccggcc agaggcgttt cccgactcct
    43801 gccgcgagga tgatggcctt catgcgcgcg ccgccgctcc tgcgagaact tcctcgaacg
    43861 cacccaggaa tccattgatc tgagattcgg tcagagcgcc catattagcg acacggaaca
    43921 ccttcgactc gagttgccct tgaccggcgt agatcacata gccgcgggcc ttgagctgat
    43981 catgcagggt cgtgtagctc agacccgcag gaagatggaa ggccgtaatc gtattcgact
    44041 gcacgtcggc cggcaggaac ggctgaaccc ccatgcccgc catccgctcg cgaatcctcg
    44101 tcgccatgcg acgatatcga tggaagcggc tggtcacccc ctcctccagc aattcgtcca
    44161 acgcctcctc gaacgcgtag tacacctgca ccgccggcgt gaacggcaca atccctcggc
    44221 cctgttcatc aacataatgg gtcagcgtca gataccagga ccgcttcgga tatttccgca
    44281 tctgatccag gaaccccttg cgcagcaaga caaacgacac gccggggaat ccctgaatgc
    44341 acttgcccgc cgttcccgcc accatataga tgtgcgaccc ggcgatatcg atcggttcgc
    44401 ccgcgaggcc actgacggaa tcgaggacga acacccggtt gagactgtcg acgacctccg
    44461 caatttcttt caccgggttg atcaaaccgg tcgtcgtctc atgatggacc atcgataccg
    44521 catgcacttc gggatgctgg cgaagggcca accggagtcg ttccggatca ggcctgacgt
    44581 gccattccga tttgagttcg gacactccga gtcgctgcaa cccgatcata ttggacagtc
    44641 gttcgccata gaccccattg ttgagcacca acatgcgctt gcccaacgga aggcaggaca
    44701 tcactgctga ctcgacggcc gccgtgccgg accccgttaa aatgacagcc acgtactcgg
    44761 actccgcacc gggcacgaag gcggtgagca gcttctgctg aatgcgctgc agcatgtcgg
    44821 aaaattcgga ctcccgatgg cagatgtccg gacgcagcag cgcctgccgc acccgttcgc
    44881 tcacatttac cggacctgga ttgagaagta tcatggttgt gcctcaggct gttgatcgga
    44941 acggagcagg acgggctcac tcagcacgtt cagtgcgccg caattactca atggctttca
    45001 tgaaacgcgc ggtgatggcc ggcggctcca atgcaatccg tccggcatct tcgacaaact
    45061 cgttcacctt gacgagcaac atactgggcc cgtcgttctt caacatatct ttgaattcgt
    45121 acacgagatc ttcacggtcc a
//
LOCUS       DAOQDS010000033        44454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_270_length_44454_cov_18.936755, whole genome shotgun sequence.
ACCESSION   DAOQDS010000033 DAOQDS010000000
VERSION     DAOQDS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 44454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44454
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_270_length_44454_cov_18.936755"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..286)
                     /locus_tag="PK589_09870"
     CDS             complement(17..286)
                     /locus_tag="PK589_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19950.1"
                     /translation="MKLFALAVVSSTTLLLGSTGTAFSAGASSDSNSVKEASPSAQLI
                     LAKQGRGGHDDPPGDDHDGVVLAKQGRGGHDDPPGDDHGDHVIIG"
     gene            complement(399..881)
                     /locus_tag="PK589_09875"
     CDS             complement(399..881)
                     /locus_tag="PK589_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19951.1"
                     /translation="MLFHTSASGIEQPSALQADTALSARLLVSWLVFWILALPLMHVH
                     IQAERHQGLPHNVLAGDLPGEFVSSADSAIEAAQPAGVPPPPHLASGISYPELAFTAP
                     VPSKHHWLFSLTAIFTRDIPAEMFPTGRTVTPDQRSFDHPTPLPLSSPRAPPPVDRLT
                     "
     gene            1370..1708
                     /locus_tag="PK589_09880"
     CDS             1370..1708
                     /locus_tag="PK589_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19952.1"
                     /translation="MKTIVLCVCMSLLLPVMSEARCRTIGESASLGSEYGTFPLYPAE
                     QVVEAGQKRKSIAMQWLGYGIGVPLFVVAMPFAMVGAVAGAAAHPWTKCKAMEGRFEG
                     PEVHLAGADR"
     gene            complement(1684..2703)
                     /locus_tag="PK589_09885"
     CDS             complement(1684..2703)
                     /locus_tag="PK589_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNJ19953.1"
                     /translation="MDVLSEVLKAVKLDGAVFFHGEFAAPWCAQEPDAETMASYLPTQ
                     AGHIIIFHLLLEGRGYIRLGDEDRAVPLTAGDIIILTQGDAHYMGNGPPVTPVDTSLQ
                     IRQILAEGRMMSQLAGGGEITKLICGYLTCDAQLCRVVLAGLPAMLKVNIRDTPSGHW
                     LENTLRYSVDHAEASGPGGAAVIAKLSEALFVETLRRYIAQLPHTETGWLAGVRDPDV
                     GKALALLHQRPAQPWTIATLAAEVGVSRSVLAERFQHFLADTPIGYLTRWRLHLAAQL
                     LTSTSKSVAEVAGDIGYESEPSFNRAFKREFNLPPARFRAQSKASLAGNGAVSRQDHR
                     SAPAR"
     gene            2899..3720
                     /locus_tag="PK589_09890"
     CDS             2899..3720
                     /locus_tag="PK589_09890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ19954.1"
                     /translation="MTTNTIMPELDSLKTRLNTIWTAGDYDRFSRYMEGGAREFYERL
                     QIAPGCRLLDVGCGSGQLALIAAKDGLEVTGVDIAANWVERAQARARAEGVSARFEVA
                     DAEALPYETASFDAVTSLIGAMFAPRPDLVAQELLRVCTPGGTLAMANWTAEGFVGQM
                     FKNVAKFIGPSGMPSPVLWGNEATVQERLGHGLSTISLTRRQYLFSYPFPPSEVVEFF
                     RLYYGPTNRAFASLEAEGQAHLRRDLETLWAAHNQAGPDCTTVYGEYLEVIGIRS"
     gene            3752..4102
                     /locus_tag="PK589_09895"
     CDS             3752..4102
                     /locus_tag="PK589_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNJ19955.1"
                     /translation="MTNATTTEIDQTTGLYDMTNLGRVKDLGAHAPEAMKAFVAFDKA
                     ALAPGAIPGKYKELMALAVAFTTQCPYCIELHANKAKEYGASAAEIAESVLVTAALRA
                     GGAVTHGTHALKGI"
     gene            4233..5408
                     /locus_tag="PK589_09900"
     CDS             4233..5408
                     /locus_tag="PK589_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNJ19956.1"
                     /translation="MKLYRSFILTLGLLLACNTLASAEILALLNYESKPDQTVRREGI
                     AIMDIDPESGNFGKILMEIPLPPDLVAHHIFFNRDRSKAYVTALGKSILHVVNLTEFP
                     YRLRAIDVPDCQMGEDLAVSEDNRTWYLTCMGSNNVIMGDALLDKPIKTVSAAEPALA
                     TIQYPHGIAIHNGIDRVLVTSTVKPDMSDAGDSITVMEAGSGRVLSTHKVSAKPSPAK
                     AAPVEVMFSPNSNPPAVHITNMLEGTLWAGLWDPASKTFSFHQVDDFGPRQQGMPLEM
                     LYNAKGDRLFVTTAKPGFVNLYDNSDPGQPKFLKTIAAAAGAHHSVLSPDERYLFVQN
                     SFLNLDGMSDGSITVIDLKADTILGNIDTLKAQGFNPNCIMLLPTQPGDLRASRVTE"
     gene            complement(5617..6843)
                     /locus_tag="PK589_09905"
     CDS             complement(5617..6843)
                     /locus_tag="PK589_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ19957.1"
                     /translation="MTTQASPSSDDQSDTPTPHRSPDPAPDGPRPAATELDRFFELSL
                     DMLCIANADSYFTRLSPAFTRTLGWSLEELMSRPFLDFVHPDDRAMTLLEVERQIGRG
                     EPVLEFQNRYQHKNGSWRLLSWKSSPHPDGRMYAIARDVTDREQAQQALRRSHEELEH
                     RVQERTAELQQRNRDLETLLYVSSHDLREPLRSISNFSQLVRDRYGDRLDAKGLDYID
                     RVVRAAQRMDQLMEDLLTLSRAQRLEMPYEDVPGEDVVKAALTQLDETIRRTGARVQV
                     AANFPWFRVNKTWITQALYNLIANALKFHLAGKVPEIEIAPFYLTEETVPLAGIVVRD
                     RGIGVEPDQAQRIFQLFQRAVGREVSGTGAGLAIVQQIAHRHGGRAWVQPREGGGSEF
                     VLLFGSQSVIGKKPAS"
     gene            6989..7894
                     /locus_tag="PK589_09910"
     CDS             6989..7894
                     /locus_tag="PK589_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19958.1"
                     /translation="MRRTWVGLAAVFMLAMVIGVMPAVVRADCVDGVRDPTSEELAFA
                     ARAEAALAAALPSPIANSERRGGTYDFSRQPRLSFCTSNQVGAFIPNVSGGYLYKFPK
                     AEADRLYAERKAVEKQIEEAEKLPPEKDAEYKQVLAQMKAAYDAAPRRSRKDPPFTPE
                     QQEQVTRAMAEGKKLEDAAKKLLSDHVASVKSQTDPLRAQAKRLESYPQDLAVRLAMN
                     MERFPEPGPTIATFGAPSARRSGGLAVHNVVIAVEGPEGAARQALFDAVDKGYLQGLV
                     GQPLPELEASKARAGRDTQAASAAK"
     gene            7949..8344
                     /locus_tag="PK589_09915"
     CDS             7949..8344
                     /locus_tag="PK589_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19959.1"
                     /translation="MDQQHQRSRGTGGGLFAGFLVGFLWFFLSFAAMFNGVFFLLLLV
                     PPAYALSGALLAWFFRLPPVHAACSLVGSALPWVAWLWPATMSSSGWMKSLVWPVFVG
                     TAFGMSLLGARLAAARRRRKPTQSDPALA"
     gene            complement(8431..9120)
                     /locus_tag="PK589_09920"
     CDS             complement(8431..9120)
                     /locus_tag="PK589_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HNJ19960.1"
                     /translation="MNVRHRSITHACASITLTCGLVLVSLTGSGCQTAYYETMEKLGY
                     HKRDLMVSDVKKARDAQQDAKEQFKSALDRFTKTLNIQGGELQDKYEVLNSEYQQSEK
                     RAEAVRSRIASVEDVSDALFTEWEGELKQYSNASLRQKSQKQLAQTRTQYGQLIKAMK
                     RAEAKMDPVLAKLKDHVLFLKHNLNAQAIASLKSELTTVEGNIDLLIKDLNASIHEAD
                     TFIASMEKENA"
     gene            9393..9839
                     /locus_tag="PK589_09925"
     CDS             9393..9839
                     /locus_tag="PK589_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNJ19961.1"
                     /translation="MPTNTVRLHRVLRSTPERIYRAFLDADAMAKWLPPNGFTGNVHH
                     MDAKVGGTFKMSFTNFTTGKSHSFGGKYLELTPYERIRYTDVFDDPNLPGEMHVTITL
                     KQMSCGTEMTAVQEGIPEMIPVEACYLGWQESLTLLTKLVEAEIPD"
     gene            complement(9844..11295)
                     /gene="pyk"
                     /locus_tag="PK589_09930"
     CDS             complement(9844..11295)
                     /gene="pyk"
                     /locus_tag="PK589_09930"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNJ19962.1"
                     /translation="MSSSLVESTMQKTRIICTIGPATDSYEMLQKLYDAGMSIARLNM
                     SHGDHESHAKVIQHIKSLNRKVKFPIPILLDTQGPEIRTGDLSNELNLRQGEIVSVTT
                     RGPLNVEESSIHINYADLLDAVNVGDRITVDNGLINFEVLEKHERHMRCRVLDGGLLK
                     SKRHVNLPGVRVNLPAITPKDTKDILFGLERDVDFIALSFVREAGDIQQLKALMGDKV
                     GRVKIIAKIEDQEGVKNLESIIKESDGIMVARGDLGVEINLEDLPNVQRTIVRLCAEY
                     GKRVIVATHLLESMIHHPHPTRAEVTDVANAIYEEADAVMLSGETTVGKYPVKCVEYL
                     RRIALKSETIPGLQFAKRLRDVGNKQQLAAAAVQLAEGVKAKGIVVITRRGIMADLVA
                     NCRPFVTNIYAFTNMSQPRRTMLLNRGVFPFKIDFSSEPEKTLQTAFRILKDRERFQA
                     GDKVVIISDVLAQQRVDSIQIRDVPSDDLPVDA"
     gene            11767..12975
                     /gene="ilvA"
                     /locus_tag="PK589_09935"
     CDS             11767..12975
                     /gene="ilvA"
                     /locus_tag="PK589_09935"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HNJ19963.1"
                     /translation="MVTVQSIAAAASRIASSIYESPLVHSRTLSRLTGNTIYLKLENL
                     QMTGSFKERGALNRILMMTEEERRRGVIAASAGNHAQGVAYHAVQRNIPVQIWMPRST
                     PLVKLSATRNHGADVVLHGENYDEACRAAMERSEERHATFIHPFDDDEVIAGQGTLGL
                     ELLRQNPTLDVIVVPVGGGGLIGGVGCAVKEQASNVEVVGVQTARLPSMKAALGSSIP
                     VELPASPTLADGIAVRTAGSRTLPLVQRYVDQMVTVDEDEIAAAILRLLEGEKTVAEG
                     AGAVALAALLQGNTGHRGKNIAVLVSGGNLDVNLLARIIERGMVRDGRRLRLRVLLPD
                     YPGSLEGLTSVIAKASANIVETSYNRAHYGVGLNEAALDVTMETRGREHASELLAALS
                     GSRYEFSVIE"
     gene            complement(13040..13726)
                     /locus_tag="PK589_09940"
     CDS             complement(13040..13726)
                     /locus_tag="PK589_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNJ19964.1"
                     /translation="MSHNLKLSLCLNLILLGFILTGYVGPSVKTTFMPDVEAPVIGNH
                     TRIHPLGIVIGSVTLGDRVFVAPAASVRGDEGRHIHIGNESNVQDGVVVHGLETFEGD
                     HELPENEVEVDGMKYSVYIGNRVSLAHQSQVHGPARVGNDTFIGMQALVFRAEVGDRV
                     VVEPGAKIIGVKVPSGRYVPALSVVTTQDVADNLPLITERYPYRNLNGGVVRVNVQLA
                     EARQPQPIAR"
     gene            complement(13919..14437)
                     /locus_tag="PK589_09945"
     CDS             complement(13919..14437)
                     /locus_tag="PK589_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19965.1"
                     /translation="MSHEQIAAPGPEWGIVIGSVLVRQEQKGSGADTAVRDQSTMFEV
                     EVVQVQPGDPNGESPYAARYHVDATAGLDRPFLARLRPGRYLIKNFYRTGMAGTGGDL
                     NLVFESQSGRVVYIGQLLVEVPPQLTHGKDYRFSVHDTHDITLSQLAPHHRDLTQQAT
                     TVPMQIREKDSH"
     gene            complement(14630..15370)
                     /locus_tag="PK589_09950"
     CDS             complement(14630..15370)
                     /locus_tag="PK589_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ19966.1"
                     /translation="MAASQTYTVTILLDVNEAQDVDDRAESRSYLVADHSSSQAVIID
                     AVLENIERDVRILKELGLTLAYAIETHVHADHITAASLLKDRTGAQIVYGGGAAGTVM
                     GADLFLSDGQTLRFGGSALTALATPGHTDGCTSYLLPGAVFTGDALFIRGNGRTDLQG
                     GSAATLFDSVRQKLFALPDDTIVYPGHDYQGRVASTIGEEKRFNERLNLSIDKDTFIE
                     LMNRRKQPRPAKIDIAVPANLRAGRMAR"
     gene            complement(15546..16502)
                     /locus_tag="PK589_09955"
     CDS             complement(15546..16502)
                     /locus_tag="PK589_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HNJ19967.1"
                     /translation="MAVTRRNILTNANVRAQYARGVNLLKREFTGPTTSSLGIPGPAR
                     QISTYDLFVVWHHTAMSTMTPPSQGDRNAAHRGPVFFPWHRFMLIELERNLQRVLRDD
                     TFGLPYWDWAADGSRTPGQQRQSALWRASGVGGTGSPVRTGPFRFVADDPDSFRIRVE
                     ADANGRLIQSNRGLRRDLGVNIATLPTRTDTAGALALTLYDQPNWDTTSAGFRNRAEG
                     WAAPDAPALHNRVHVWVGGDMLPSSSPNDPVFYLNHCNEDRLWEAWMARHTRAYVPTQ
                     SAPASLRGHRLNDQMLAFVSAAMRPADVLDVGSIYVYDSLAV"
     gene            complement(16512..16892)
                     /locus_tag="PK589_09960"
     CDS             complement(16512..16892)
                     /locus_tag="PK589_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19968.1"
                     /translation="MANRKREPVPTPQSIASYVKRHAPRVKQSQRRHHAATETIREAD
                     HQGHHIVVKTRYEISVDGRKVLGHLGVSNDGHVHYHPIPNLSFASALDMVKAIIDVFP
                     HQFEAKKRPHKGPGRIRHTNRTHH"
     gene            complement(17005..17523)
                     /locus_tag="PK589_09965"
     CDS             complement(17005..17523)
                     /locus_tag="PK589_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19969.1"
                     /translation="MDIVSHGLWGALAFGRRSRTSFWLAFGIGLAPDLFSFGILWAAA
                     TLGLSPKPDFSHGTPPESTIPPYVHHLYDVTHSFVVFLAVFALLWLVMKRPVWELGAW
                     GLHVLVDIPTHSFAFFPTPIFWPLSDWKFDGWQWSQPAILIPNFTVLLLLYGWFLFQQ
                     ARPILRPERARD"
     gene            17664..17888
                     /locus_tag="PK589_09970"
     CDS             17664..17888
                     /locus_tag="PK589_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HNJ19970.1"
                     /translation="MDQNFKVGDHVSWNSEAGRIRGTIQKRITAPMKFKSYIARASKE
                     QPQYLVKSDKTGHLAMHKGTALRKIGGQSA"
     gene            complement(17885..18313)
                     /locus_tag="PK589_09975"
     CDS             complement(17885..18313)
                     /locus_tag="PK589_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HNJ19971.1"
                     /translation="MTTIDPTRHEWARFERLIVFDGICNWCNAWVNFTMARDRGRFHF
                     ATLQSAKGQELLTLLGLPVQDFETFLLLERGQVFTKSTAALRIARTLSGWWPLLYGFI
                     LIPRGLRDILYNYVARHRYRLMGKADTCRIPSATDRDRFV"
     gene            18455..18757
                     /locus_tag="PK589_09980"
     CDS             18455..18757
                     /locus_tag="PK589_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19972.1"
                     /translation="MAFNQVGDFWLAPGEAIRVHIARGKLVPDEEWGGEDFGAQWIMA
                     DGVGINPVRLMVSEHTKEKKALRRHPGPSGPVVYSVTVTNIGEELAHFTVQGGGNV"
     gene            18779..19042
                     /locus_tag="PK589_09985"
     CDS             18779..19042
                     /locus_tag="PK589_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19973.1"
                     /translation="MKTKRTMHVLTNKKGAIVGGGLLTPGKDHKGKPVHIQIEPMKGQ
                     SLKEVAVPDELARLEGADFFSRLMCDFHLPRGKKELVRRGSKR"
     gene            19357..21009
                     /locus_tag="PK589_09990"
     CDS             19357..21009
                     /locus_tag="PK589_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNJ19974.1"
                     /translation="MTLLQPNTPWWQSAIVYQIYPWSFQDSNGDGIGDLPGIISRLDY
                     LNGSPDSLGVDAIWLSPIYPSPMHDFGYDVSDYCDIDPRFGTLADFDRLVSEAHRRGI
                     RVVMDLVLNHTSNQHPWFIESRSSRTSAKRDWYYWADGKSGRRPPNNWAARFGGSAWT
                     HDPHTGQYYLHSFLSEQPDLNWTNPAVRDAVFGVVRFWLDRGVDGFRLDAINWLGKDT
                     SWPDNPARLAWRSYYRQIHRYDRDQPHTHEALRALRSSLKDREDILLVGETSSDTPGG
                     PAAFYGNGSNELHEVFDFRLLRSDWHPDTFRRLILDSDRAVPQGGWPPVVFSNHDQSR
                     HIDRYGAGGEAIRRARATALLLFTLRGTPFVYYGEELGLHDGRLRRADLHDPYTIRYW
                     PWKTGRDPARTPMPWDDSPQAGFTTGRPWLPLSQAWRQTNVAGEQRDPGSILSLYKRL
                     IRMKKGSNALTVGAYHPVDGGPPECLFFTRESLVDGQRDALFIAVNFSAHTQKISLPA
                     GPSKLGRHGTLMLSTDPLRAEGPWTADHFLLGPDEGIVVSMQ"
     gene            21077..21958
                     /locus_tag="PK589_09995"
     CDS             21077..21958
                     /locus_tag="PK589_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNJ19975.1"
                     /translation="MRIATWNVNSLKARLEKVLWWLDRAKPDVLLMQETKLSDADAPI
                     DAFAQAGYALAHHGEGRWNGVAIASRCGISGVISNFGEPLQPARSTDTGDDEPFAEAR
                     MLAADCGGLLIICLYAPNGREVDSPFYEAKLVWFDKLARWLKQTGAPQKACVIGGDFN
                     VAPQDIDVWDPAACHGGTHVSDRERRAFDRIVREGFVDAYRCHHPEPGRYTWWDYRAG
                     NFHKNIGMRIDHLLVSTPLRDRLIWSEIDREARKGKPLPSDHAPLVIDLDTAGHPFDA
                     GWVSAESRIAARTGKKR"
     gene            complement(21983..22354)
                     /locus_tag="PK589_10000"
     CDS             complement(21983..22354)
                     /locus_tag="PK589_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19976.1"
                     /translation="MKRDTWLVVTAVTGGIVVWLLVSAFAHKREAWDSDLYFTLGLPA
                     VCLLSALLGYLEPNRAWRWGAAPLVGQALVMLLTQGPGTVLPLGIMMFGVLALPSIVT
                     AKMGAYAAKRSLHRSTARQNH"
     gene            complement(22522..23838)
                     /locus_tag="PK589_10005"
     CDS             complement(22522..23838)
                     /locus_tag="PK589_10005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ19977.1"
                     /translation="MTLSRKTIHDSTGLLGELLAQYHPRNFDIRLWDGTIWPADPGAT
                     PEFTLILRTPTVLRDMLWKASELALAEAFISGDLEIDGDLEQAIPLAEYLSRHPVTWG
                     SWLRLGFKSLRWPSNPSFTTNGLAAHVTGPNHSLLRDKQAIAYHYDHPPEFYAGWLDP
                     QMVYSCAYFSFTDDLDAAQVHKLDYLCRKLRLRRGERLLDVGCGWGALIVHAARYYGV
                     QACGITLSEKQAEFAEACIQQHGLMDRCQVELRDYRQLSTPNRYDKIVSVGMVEHVGA
                     THLSGYFRHIWNLLKPGGTFLNHGIGIRHGERCAFGPFVNTYVFPDAELTTISTTTGF
                     AETAGFEVRDVECLREHYVLTLRQWRQRLEKNVTPGSSSMDESTWRLWRLYLASAAHG
                     FATGKLNLYQTLLSKTQEGSAGLPLTRDDWYEAHSSIDSRLSGRLR"
     gene            complement(24049..24459)
                     /locus_tag="PK589_10010"
     CDS             complement(24049..24459)
                     /locus_tag="PK589_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19978.1"
                     /translation="MARWSRVWLGPEGLWLMISLAVYVAAAMNQPSTPAGNDFLETLW
                     VAIPLVGIPLTFATALLPADTGWWWLVRVIVASCIGVIVASFIAAGGVDYHDSRNSGL
                     LGAPVYSLSIGLLLLFPLTILAILLIWKKRRTSR"
     gene            complement(24539..25543)
                     /locus_tag="PK589_10015"
     CDS             complement(24539..25543)
                     /locus_tag="PK589_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HNJ19979.1"
                     /translation="MWRSRIATAALAVALISPLSVLAEEPPANPKSLAEQLVDAFNGV
                     FGVHPGMRANHPKGMVLEGTFTPTASAASVSKAAHLQKKKTAVPVTVRFSAGSGQPSV
                     PDTSQMPRGMAVKFSLPDGSKTDLVVLSFNGFPVATAEEFRDFLLAVGASGPDAPKPT
                     PIEQFLATHPAAKHFVESPKPQPVSYATLPYFGINAFKFTNAKGHVTFGRYQLRPMAG
                     EHFLTQEQLNTMDPDYLSAEIRERLRRGPAKFTLLLQVAEKGDKIDDPSIAWPDSRKQ
                     IKLGTISITKAVADSQAAEKKLLFMPGALVPGIEAADPMIAARSASYVVSLSRRAQAQ
                     "
     gene            25979..27037
                     /locus_tag="PK589_10020"
     CDS             25979..27037
                     /locus_tag="PK589_10020"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ19980.1"
                     /translation="MMAFSLKPLRQQIIVITGATSGIGLTTARMAARRGARLVLAGRT
                     EGALTELERELSQGQHRAVTVTADVGKAADVQRISRVARQMFGGFDTWINNAGVSMYG
                     RLLDEPIEDMRRLFETNFWGLVYGSLEAARYFKAHDQAGAIINVGSTLSDRALVLQGM
                     YSASKHAVKAFTDALRMEVEMDGIPISVTLIQPGAIATPYSQHAKNYMKTEPTNPPPV
                     YSPETAAQTILYCAENRERHVYVGASGKGIGMMGYYAPRLADLLMETLFARQQQSDRP
                     PARPRRDNALDRPSGDLRERGDYQGHVSESSLYTTASLHPMLTGAALVGAGLALKTLW
                     PSKHTSERVQNAPRAQRR"
     gene            complement(27062..28306)
                     /locus_tag="PK589_10025"
     CDS             complement(27062..28306)
                     /locus_tag="PK589_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNJ19981.1"
                     /translation="MDDNALENTAIVTSQTGVVARWQQIPLYGRIIIGVILGLAAGLA
                     LGNQAAFLATPGKLVLRLLGALAPPLILVAIVHTFMTTHLGGPLAARLPRLLLMNTLV
                     AITVGLTVANVIQPGRGAGLAPAEPAVETTHTANPLVAFIENVPKSLLGPLGDDGKVI
                     GVIFVAVAFGMALRKERHRPLGTVEHLVELVLESLIKILHWIIAVVPLAVFGIVASIV
                     GTEGFAQFQALGIFVLSVLLALTIQAAYYLVRVRFGSWVRPGELLRGGRDALVMAFST
                     ASSTATMPVTYAALKDRIGLREQSASMGALVGANFNNDGTALYEAMAALFIAQMIGID
                     LSMHQQVMIVLTSIIASVGAAGIPEAGLVTMTMVFTAVGLPVQFIPVLLTVDWFLDRC
                     RTAINVMGDMNVSCLLDGKQKG"
     gene            complement(28597..29415)
                     /locus_tag="PK589_10030"
     CDS             complement(28597..29415)
                     /locus_tag="PK589_10030"
                     /inference="COORDINATES: protein motif:HMM:NF039346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ19982.1"
                     /translation="MVSVPLTNLKNDSINSDDAILLFENGGHKVYWIGTHTGGDEIEC
                     NTYLIIDGHEGYILEPGGFDRFPHVFKKVCLLLSPNSITHLFFSHQDPDICASFPSWT
                     KWNDDIKLVVSSLWTRFMLHYESRDLSGKAHNLNFVKVPDEGLSIPLNDGGRLDCLSA
                     PFLHSPGNILVHDTVSGFLFSGDVGAAMYKDHTFRLVIDDWNAHTQAMQGFHQRYMSS
                     NRAVAGMNKQLAPLPITAILPQHGCIFRGDEVSRFTQWLGKLPVGADYFYPDLA"
     gene            complement(29844..30152)
                     /locus_tag="PK589_10035"
     CDS             complement(29844..30152)
                     /locus_tag="PK589_10035"
                     /inference="COORDINATES: protein motif:HMM:NF018599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HNJ19983.1"
                     /translation="MLELRPTCEHCNTPLPPDSPEARICSYECTFCAACVTDVLHNVC
                     PNCGGGFEPRPIRPSKNWRGENYLGKDPASTTIRHRPVDPSAHRAFAATLNAVPPEQR
                     "
     gene            complement(30489..30905)
                     /locus_tag="PK589_10040"
     CDS             complement(30489..30905)
                     /locus_tag="PK589_10040"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ19984.1"
                     /translation="MDIQYKNDTEGVDWERMSELFERVGWGKRKAEDIQAAFRSSSYV
                     RCAYDERTLVGVGRTVDDGQYYALIVDLVVDPQYQGRGIGSNILSYLEHSLANYVFTT
                     LTAAVGKEGFYLNQGWRRHTTSFIWPRTERQRKDHT"
     gene            complement(30984..31358)
                     /locus_tag="PK589_10045"
     CDS             complement(30984..31358)
                     /locus_tag="PK589_10045"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNJ19985.1"
                     /translation="MAQSKQVAALLGPSLIVVSVTELLNLEIFTNTSAHLTYLNGTLP
                     VVAGLTIVRAHNYWMPRWPVRITLTGWLLLLGGLIRMVAPVSSQQTAHHPTAVLAMTA
                     VLLVCGVVLTVNGYRQRDVRDS"
     gene            31725..32123
                     /locus_tag="PK589_10050"
     CDS             31725..32123
                     /locus_tag="PK589_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19986.1"
                     /translation="MQGFTPAFAYFLIVFGAGFLLGTIRVLLLLPVLGERAAELLEMP
                     FMLIVIVLAARWIDRRFLAGVDDRGRIVVGLLAFGLVLSAEFVVGIALRGMTPRDVLF
                     ARDPVSGAAYYLSLLLFAVMPWLIAPRRRR"
     gene            complement(32750..33208)
                     /locus_tag="PK589_10055"
     CDS             complement(32750..33208)
                     /locus_tag="PK589_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19987.1"
                     /translation="MRTTVLSSELAHSTSRIGFMRSVFELLGIVLVRFRPVQRMWGAW
                     LIAVNAACLLFITHIEAQVALAAVGLALLAQALIYQRTRFIRLLGVTHLIWVPMLAWM
                     AVRLAAVPEEDVAFQAWLLALIATNAISLAIDAWDATRFVRGERQPHYVW"
     gene            33602..34486
                     /locus_tag="PK589_10060"
     CDS             33602..34486
                     /locus_tag="PK589_10060"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNJ19988.1"
                     /translation="MKDNKTAVLRKLTEDSAMQLLEGVTLPPQPGTLLDINRERAKAE
                     PDVAAVAGIIERDLSVAAAVLKTINAPWFELPKKVESVRQAVVLLGMPNVVNVVIALA
                     MKSELQGNNQAGIDRFFDEANDTAMVLSSVMREVPVMSTDKAYLLGLFRDCGMPILLQ
                     RLPQYAEVLQEAAKRPEQSLPDIEDARLKTDHAVVGALMSRHWHLPEEIGTIILNHHR
                     TRELFGGKAQKLASSEESIRTVIAVLAMVDCICHRLRTRNDSPEWAAIGEKVLAHLKL
                     DQAEFEHLAWDQQDRFAA"
     gene            complement(34888..35484)
                     /locus_tag="PK589_10065"
     CDS             complement(34888..35484)
                     /locus_tag="PK589_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HNJ19989.1"
                     /translation="MKTAWMALLALCAVAQTGEAALIGTDVMFQSVFQATSTSPEFKL
                     TSLNTVTVKEPGVEFPSLKALQVSNPSGLRLVDIAINIGDTFIDMNFTNSAPDFRFAS
                     GFRNGYKLTFDNAAMPSIRQALIDRTVTTLGLSDSDVTFSGNTLQVNVESLPFNTSTF
                     ARINLTVEGGPSPVPVPAAAWLFGSGLAALVALKRSGA"
     gene            35855..36346
                     /locus_tag="PK589_10070"
     CDS             35855..36346
                     /locus_tag="PK589_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19990.1"
                     /translation="MPDRFHPDEALKNEAISQAMEGDFIGARQTGAKIAARQDFLEAW
                     MYILHLQRDRGDVIGIKETILSCPNPSWLQGHSYAELARHFAKIGNKTAAIEIAKLLG
                     RSGEMMVVYLSAPVDLILKGDFAGAREAVAKVEDEQLRNQLNALVDREEQIRSGPTQQ
                     SVL"
     gene            complement(36669..37391)
                     /locus_tag="PK589_10075"
     CDS             complement(36669..37391)
                     /locus_tag="PK589_10075"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNJ19991.1"
                     /translation="MPTHDTFLSYASEDNDFAFQIAYGLRANGISVWFAPLMLKVGDR
                     LLDSVELGMAESRTGTLLLSPTYLHKGWTSYEMDILIRQHIEGTKPLLPIWHNASKKQ
                     VDAKHSGLGGIVAITDTSSATEVVFKLIEGLSSGAPSRAVVPIWEDPTYRFLEGLAEV
                     NLQSGEGAATTIFEFLLHAKESQYPLWIGGRVFTKNQLLLHVAQLLGPVPDRVKSWVH
                     EDGYRKLWEMCVENDLNPHNFY"
     gene            complement(37437..38333)
                     /locus_tag="PK589_10080"
     CDS             complement(37437..38333)
                     /locus_tag="PK589_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19992.1"
                     /translation="MANQDETLKHIWKSGFPFQLKIEDEVRRTHSTHNWLIATREHPW
                     TNKETESSGFIDLVLMHDHFVTYRMVIECKRVKADDRRQLHWLFLIPEGEKQETPLAS
                     CLQIGGTSPMNARIGEANVSQQEWDHVRIWENVNIFPKSFESSICVLPSDDSKSQPIL
                     ERLAAGVIDSVEGLGDEEILVHQSQFQVDRLIAFLYPVIVTNAEIAICSFDPSKVSIL
                     DGTLDASAATITTVPFIRFRKSIATTFPEGTFSNLKEANEARELTVFVVNAGALPSLL
                     TNWRMASRDLSGRYAIQNHLQR"
     gene            complement(38701..39027)
                     /locus_tag="PK589_10085"
     CDS             complement(38701..39027)
                     /locus_tag="PK589_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HNJ19993.1"
                     /translation="MLITQSIALFVLAGLFEIGGGYLVWQWWRNGSHWSVGLLGAVIL
                     ILYGIVPTYQPSHFGRVYAAYGGWFIILSILWGWLVDHVEPDRFDIIGAMVCLVGVTV
                     IMYWPR"
     gene            39213..39758
                     /locus_tag="PK589_10090"
     CDS             39213..39758
                     /locus_tag="PK589_10090"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNJ19994.1"
                     /translation="MKLLSVQVGRPRTVRWHGKVVSTGIYKEPVEGRLPLQLFNLKGD
                     EQADLTVHGGREKAVYVYPSEHYPFWRKEYPDLPFHPGWFGENFTVEGMDERTVCIGD
                     QFRIGGALVVVTQPRMPCFKLGIRLGKPDAVKRFHASGRCGFYLGVLQEGEVGPGDLW
                     EQVARGDEQMTVIESYRLMTQ"
     gene            40050..40250
                     /locus_tag="PK589_10095"
     CDS             40050..40250
                     /locus_tag="PK589_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ19995.1"
                     /translation="MSKVEAIEQQIEKLSSDELAAFRRWYVAFDAEAWDRQFENDVKA
                     GRLDALAEKALRAHTSGQSRPL"
     gene            40960..42858
                     /locus_tag="PK589_10100"
     CDS             40960..42858
                     /locus_tag="PK589_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249307.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNJ19996.1"
                     /translation="MRVEYTKGERASRELIMLQRQSSEAAAGRKMKVMLIFPPDWFPS
                     EPYLSLPSLTAVLRQAGHQVVQKDINLEMWDWYFSEDFLKKVLRKVPQQLDRLRKLAK
                     KRELEDWEQDLQLQLCEVSRQRIDELIKKAEQAKAIIRGEIFYEIDQLEWAIQVFREV
                     TSVISLVYAPARICMPPMETNLSYKVFVSSEVIEAVNDTQVNVYRDVFEQLVKPAIEA
                     EQPDVIGISIVLQQQMFSSMTFCALIKQHFPHIHVTIGGNTVTRLRDVLPESPLFQYF
                     DSAVVYEGETAFVQLVSAVGAKQSLAEVPNTIYKDATGVHVSTTSFAEDMHSLPPPDF
                     DGLPLEKYFVPTKILPYLATRGCYWGRCEFCDHGEGYTAGYRSKKIQDVLAEITFLRD
                     KYGARHFHFTDESYPPALFRKLARGLIDSNMGIVWTTHMRFEKSLLEDQVWQDAKESG
                     CKYLHFGYESGNERVLKLMDKATTTAIMTEHLKRTAEAGIWNHCMGFFGFPGETREEA
                     WSSVQFLEQNKDYVHSLGFGTFDLGRHNPVAKHPEKFGVTAYKNPEWDLALDYYFTVK
                     QGLSIEEAERVFEEFERNHNPGWDLRLFIREYIFLYIARFGLQKLPDLQYRSAKIIGA
                     SPTLAGKM"
     gene            43052..>44454
                     /locus_tag="PK589_10105"
     CDS             43052..>44454
                     /locus_tag="PK589_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ19997.1"
                     /translation="MQRDINIEMYDHFFSMEFLIWVKGRLGMHLKPLQDKEKAGTLTD
                     QEASQKAVLEDAYAVDVFDLAERAEDAKLVVRGERFYEAEKLERALNTFREAMQYISA
                     AYYPASLVFYPMESNLGYRPGVSKEVFACLDDEQVNVYRDICNQLVLPSVSKEKPSVI
                     GISIGTQMQLMAGLTFCKMIKESFPEIRVVVGGNVVTRLQEEWAHHERFFTEVFDAAI
                     LYEGEHALLWYLEAVNGQRAIDQVPNLMYRDADGVKQSKEVYTEKTSALPLPDFDGLP
                     LDNYFVPERIIPYLATRGCYWGRCTFCDHGQGYFDQYRGMSAQHVVEQVTALRDKYNC
                     RHFLFSDESYPPALFKKVSQLLVDRNVGIKWTTLIRFEETLQDQAIWDLAAKAGCCTL
                     YYGMESANERVLNLMDKHARKSVIQNNLHQAAKAGIWNHVMAFYGFPGETRDEALETR
                     QFVIDNQPVIHSVELFY"
BASE COUNT         9282 a        12462 c        13403 g         9307 t
ORIGIN      
        1 cctgtctgca caaggactag ccgatgatca catggtctcc atgatcatcg ccgggaggat
       61 cgtcatgtcc gccccggccc tgcttggcca acacgacgcc atcgtgatca tccccgggag
      121 gatcatcatg tccgccgcgg ccctgcttgg cgagaatcag ttgcgcggaa ggagatgcct
      181 ccttcacgct gttgctatct gaagatgcac cggcactgaa ggcggtgccc gtggagccca
      241 agagcaatgt cgtcgagctc accactgcca aagcaaataa tttcatgctg aatctcctat
      301 tggtcgtgcg aatgccagtc gtctcccatc catcttttga acagacggat ccctttggag
      361 catgttctcc agaatcactc tcttcagcgc gaatgatctt aggtcagccg gtcgactggc
      421 ggcggcgcgc gtggagagga tagagggagt ggagtggggt gatcgaatga acgttggtcg
      481 ggagtaaccg ttctaccggt gggaaacatc tctgctggaa tgtcacgtgt gaaaatggcg
      541 gtcagagaaa acaaccaatg atgtttggaa ggaaccggcg cagtgaacgc caattccgga
      601 taggaaatac cggaggcgag gtgtggagga ggcggaacgc cagcgggttg cgccgcttct
      661 atggcgctat ctgcggacga gacaaactca cccggcagat cgccggccag cacattgtgc
      721 ggaagcccct gatgccgctc agcttgaatg tgcacgtgca tgagcgggag ggcgagaatc
      781 cagaacacca gccacgagac aagcaggcga gcagagaggg cggtatcagc ttgaagcgcg
      841 gatggctgtt cgataccaga ggccgatgta tggaatagca atggctcctc cttcctgcgc
      901 cgaaagtgca gcgccgggga acatcgtgtc cggaacgtat gagagaaatg cagagcccag
      961 ggatccatga ttcgcatcat ggcaggtgcg ttgagcactc attcaatggg ccgggaaacc
     1021 gtattgattc accaagagga tcgaatcctc atggtgcgat ggagccgcag tgaatcgttc
     1081 tagtcaggat caactgtact aggactaata cgccggttgt cctacagaag caaggatttt
     1141 ttttgcctca tgttgagaca gtggtatgag gacggattgc gtatttattg cagcctgtgt
     1201 tttcgcacgc tgccgaatgc gcgaaagcgc tcggtttcgc ggggagatgt aaccgctcaa
     1261 atagaaccca gcgtttcgtt ggtatcaacg acaggcatgg gatgtgctgg tatcttttcg
     1321 cacccgggcg agcagccaat tgcggtggtg tttactggag ggagtggtga tgaaaacgat
     1381 agtgctctgt gtctgcatga gtctcttgct gccggtgatg agtgaggcgc gctgccggac
     1441 gatcggcgaa agtgcgagcc tgggcagcga atacggcacc tttccgttgt atccagccga
     1501 acaggtcgtc gaggcgggac agaagcgcaa gagcatcgcg atgcaatggc tcgggtatgg
     1561 tatcggtgtg ccgctctttg ttgtcgcgat gccgtttgcc atggtgggag cggtcgccgg
     1621 cgcggcagca cacccgtgga ccaagtgcaa ggcgatggaa ggccgattcg aagggccgga
     1681 ggttcacctg gcaggcgctg atcggtgatc ttgccgggag acggcaccgt ttccagccaa
     1741 cgacgccttc gattgggcac gaaatcttgc cggaggcaga ttgaactccc gtttgaaggc
     1801 gcggttgaaa gagggttctg actcatagcc gatgtcgccg gcgacttcgg ccacgctctt
     1861 ggacgtggac gtgagcagtt gcgcggcgag gtgcagccgc cagcgggtga gatagccgat
     1921 cggtgtgtcg gcgaggaaat gctgaaaccg ttcagccagc acggaccggg acacaccgac
     1981 ctcggcggcg agcgtggcga tggtccatgg ttgggcgggg cgttgatgca ggagcgccaa
     2041 agctttgccc acgtccggat cacgcacccc ggcaagccag ccggtctcgg tgtgcggcaa
     2101 ttgtgcgatg tagcgtcgca acgtctccac gaacaaggct tcggataact tcgcgatcac
     2161 cgctgcgcct ccgggaccgg atgcctctgc gtgatcgacg gagtagcgga gcgtattctc
     2221 cagccagtgt cccgacggcg tatcccggat attcaccttc agcatggcgg gcagcccggc
     2281 caggacaacc cggcatagct gcgcgtcgca cgtcaggtac ccgcagatca gcttcgtaat
     2341 ttccccgccg ccggcgagtt gcgacatcat acgtccctcc gcgaggatct gacggatttg
     2401 cagagaggtg tccaccggcg tgacgggcgg cccgttgccc atatagtgcg catccccctg
     2461 cgtgagaatg atgatgtctc ccgccgtcag cggcaccgcg cggtcctcgt ccccgaggcg
     2521 aatatagccc cgtccttcca gcagcagatg aaaaatgatg atgtggccgg cttgggtggg
     2581 gagatacgac gccatggtct cggcgtcagg ttcttgtgcg caccagggcg cggcgaattc
     2641 accgtgaaag aacacggcac cgtcgagttt cacggccttc agcacttcgg acagcacgtc
     2701 catgaacagt tcctcggcga ggccggagtc tgagcaaagt catccggacg cccggataaa
     2761 cgatctgcag cttagcatgc tcccgataag agagccagac gtgcggataa gacgcggaac
     2821 ggcccctgtc ctatagtgca accagacagc gcgcggtacg aggcacgagg acagagcaca
     2881 ttcaacaagg aggaggccat gacgacgaat accataatgc cggagctcga tagtctgaag
     2941 acccgtctca atactatttg gacggcagga gactacgatc gattttcacg atacatggag
     3001 ggcggcgcca gggagtttta cgaacgcctg cagattgcgc cggggtgccg gttgctggat
     3061 gtcgggtgcg ggtcgggcca actggcgttg atcgccgcga aggatggcct ggaggtgacg
     3121 ggagtggaca ttgcggccaa ttgggtcgag cgcgcgcaag ccagggcgcg cgcggaaggg
     3181 gtgtcagcac gattcgaagt cgccgatgcc gaagcgcttc cgtatgaaac agccagtttt
     3241 gatgccgtga cgagcctgat cggtgcgatg ttcgccccgc ggcctgacct ggtggcgcag
     3301 gagttattgc gggtgtgcac gcccggtggg acccttgcca tggccaattg gacggcggag
     3361 gggtttgtgg gccagatgtt taagaacgtc gcgaaattca tcggcccgtc gggaatgccg
     3421 tcgccggtgc tgtggggtaa cgaagccacg gtgcaggaac gcctcggtca cggattgtcc
     3481 acaatcagcc tgaccagacg gcagtatctc ttcagctatc cgtttcctcc ctcggaggtc
     3541 gtggaatttt tccgtctcta ctatggaccg accaaccggg cctttgcctc ccttgaggcc
     3601 gaagggcagg cgcatttgcg ccgggatctg gaaacgctgt gggccgcgca caatcaagcg
     3661 ggaccggact gcacgacggt gtatggcgaa tatttggaag tcatcggcat tcggtcgtaa
     3721 accccgattg cccatacaaa ggagatgcat catgacgaac gcgaccacca ccgagatcga
     3781 tcagacgacg ggtctgtatg acatgacgaa tttggggagg gtgaaagatc tgggggcgca
     3841 tgcacccgag gcgatgaagg cgttcgtggc cttcgataag gctgcactgg cgcccggcgc
     3901 gatcccaggg aagtacaagg agttgatggc cctggccgtg gcgttcacca cgcaatgtcc
     3961 ctactgcatc gaactgcacg cgaacaaggc gaaagaatat ggcgcctcgg ccgccgagat
     4021 tgctgaatcg gttctcgtga cagccgcgtt gcgcgccggc ggtgccgtca cgcacggcac
     4081 ccatgcgctg aaggggatct aaccggtgag gcgggccggg actgtcggcc cgccctgcaa
     4141 tagaggccta tcgcgcggac aacgatgtcg gagccgccac cactggaaga aggcaccgtg
     4201 agttcgctgc cgcaattgaa aaggagctca tcatgaaact ctatcgatca ttcatcctca
     4261 cgctggggtt attgctggcc tgcaacacgc tggcttcagc ggaaatactg gcgttgctca
     4321 attatgagag caagccggac caaacggtaa gacgggaagg cattgccatt atggacatcg
     4381 atcccgaatc cgggaacttc gggaaaatcc tgatggagat tccgctgcct cccgatctcg
     4441 tcgcccacca tatcttcttc aatcgcgacc ggagtaaagc ctacgtcacg gccttgggga
     4501 agagcattct gcacgtcgtg aatctcacag aattccctta tcggctgagg gcgatcgacg
     4561 tgccggattg tcagatggga gaagatctgg ccgtgtcaga ggataaccga acctggtatc
     4621 tcacctgcat gggctcgaac aatgtcatta tgggagatgc cttgctcgat aagccgatca
     4681 aaacggtcag cgcagcagag ccggcgttgg ccacaattca gtatccacac gggattgcga
     4741 ttcataacgg catcgatcgc gtccttgtga ccagtaccgt caaaccggac atgtccgatg
     4801 cgggagactc catcaccgtg atggaggccg gtagcggccg cgtgctctcg acccacaagg
     4861 tctcagcgaa accgtcaccg gcgaaagccg ctcccgtcga agtcatgttc agtccgaatt
     4921 cgaatcctcc cgccgtgcat atcaccaata tgctcgaagg gacattgtgg gccggcctct
     4981 gggatccggc cagcaagacg ttttcgttcc atcaggtcga tgacttcggc ccacggcagc
     5041 agggcatgcc gctcgaaatg ctctacaacg ccaagggaga tcgtctcttc gtcaccacgg
     5101 cgaaaccggg tttcgtcaat ctgtacgaca acagcgaccc ggggcaaccg aagtttctca
     5161 aaacgatcgc cgcggccgcc ggcgcacacc atagcgtgct gtcgccggac gagcgctacc
     5221 tgttcgtcca gaacagcttt ctcaacctgg acgggatgag cgatggctcg attaccgtga
     5281 tcgatctcaa ggcagataca atcctgggga atatcgacac gctgaaggct cagggattca
     5341 atcccaactg catcatgctg ctgcccaccc aaccgggcga cttgcgggcg agccgggtca
     5401 ctgagtgaag gaccgggtgg tgtgggtagt taaccacata cgatgaagcc ggtgtgaatt
     5461 tcagcggtct cgagagagga gggagccggt tgccgttcta cggcagcggg ctccctccag
     5521 ggcggaacct gtatcggtcg gatgtggtac gggggcatct gctgtccggg ccgccccagg
     5581 caattgagtt aacggtcgaa ctgaggagcg gtgaaatcag gatgccggtt tcttgccgat
     5641 gactgattgg cttccgaaga gcagcacgaa ttccgatccg ccgccttccc gcggttgcac
     5701 ccacgcgcgc ccgccgtgcc gatgtgcgat ttgctgaacg atggccaggc ccgcgccggt
     5761 gccgctcact tcgcgtccca ctgcccgttg aaacaattga aagatccgct gcgcctgatc
     5821 cggctcaacc ccgataccac ggtcgcgcac cacaatccct gccagcggga ccgtctcttc
     5881 cgtcagatag aaaggggcga tctcaatctc ggggactttc ccggccaggt gaaacttcag
     5941 cgcgttcgca atgaggttgt acagcgcttg cgtgatccac gttttattga cgcgaaacca
     6001 gggaaagttc gcggctacct ggacacgtgc cccggtgcgt cgaatcgtct catccagttg
     6061 cgtcaaggcg gctttgacca cgtcttcgcc agggacgtct tcgtagggca tctctaatcg
     6121 ttgggccctc gacagcgtga ggagatcctc catcagctga tccatgcgtt gcgccgcgcg
     6181 aacgacgcga tcgatgtaat cgaggccctt cgcgtccaga cgatctccgt accggtctcg
     6241 caccagttgc gagaagtttg aaattgatcg caggggctca cgcagatcgt gagagctgac
     6301 atagagcagc gtttccaaat cgcgattgcg ctgctggagt tccgccgtcc gttcctgaac
     6361 gcggtgctcc aattcctcat gggagcgtcg taacgcctgc tgtgcctgct cgcggtccgt
     6421 gacgtcgcgt gcaatcgcat acatgcgtcc atccggatgc ggcgaagact tccacgacag
     6481 cagccgccat gaaccgttct tgtgctgata gcggttttga aattccagga ccggctcacc
     6541 ccggccgatc tggcgctcga cctcgaggag ggtcatggcc cgatcgtcgg gatggacgaa
     6601 atcaaggaac ggtcggctca tcaattcttc gagactccag cctagggtgc gggtaaatgc
     6661 gggactcagg cgtgtgaaat agctgtccgc gttcgcaatg cagagcatgt ctaaagacag
     6721 ctcgaaaaag cgatcgagct ctgttgctgc cggccgtggc ccgtcggggg cgggatcggg
     6781 tgagcgatga ggcgtcggtg tgtcagattg gtcgtcagaa gaaggcgagg cctgtgtcgt
     6841 catggatgga tcttagtcaa acaggtagat tcccgcaaga attctgtacg ccggcgacct
     6901 tgtacacgtt gacttcgttc acccattgcc tatactcagg tcgtattggc tgggaattgc
     6961 tattggcact cagagtggga gggctgcaat gcgtaggaca tgggttgggc ttgctgcggt
     7021 attcatgctc gcgatggtca ttggtgtcat gccggcggtg gtccgcgcgg attgcgtgga
     7081 cggggtccgt gatccgacat cggaagaact ggcgttcgca gctcgcgcgg aagcagcact
     7141 ggcggcggca ttaccatctc ccattgccaa tagcgaacgg cgcgggggaa cctatgattt
     7201 ttcccggcaa ccgcgcctct cgttttgtac atccaaccag gtaggggcgt tcattcccaa
     7261 tgtgagcggg gggtatctgt acaaatttcc caaggcggag gccgatcggt tgtacgccga
     7321 acgcaaggcg gttgagaagc agattgaaga ggcggagaaa ttgccgccgg agaaggacgc
     7381 ggaatataag caggtactcg cgcagatgaa ggctgcctac gatgccgcgc cacgtcgcag
     7441 ccggaaggac ccacccttca cgcccgagca acaggagcaa gtgactcgtg ccatggctga
     7501 gggaaagaaa ctcgaagacg cggcgaagaa gctgctcagc gaccatgtcg cgtcggtcaa
     7561 atctcaaacc gacccactgc gcgcccaagc taagcggttg gaatcctatc cgcaggatct
     7621 ggcggtgcgc ctcgcgatga acatggaacg attccctgag ccaggcccta cgatagcgac
     7681 attcggcgcg ccgagcgccc gccgtagcgg aggcctcgcc gtgcacaatg tggtgatcgc
     7741 cgtcgaagga ccggaggggg cagcgcgcca ggctctgttc gacgcggtcg ataaggggta
     7801 tctccagggt ctcgtcgggc agccgttgcc ggagcttgaa gcatcgaagg cgcgggctgg
     7861 gcgagatact caggcggcat ccgcagctaa gtagacgccg aacctaaaat tccttggtgc
     7921 gctggtggtc acgggataca ctgggaccat ggatcaacaa catcagcgga gcagaggaac
     7981 gggaggcggc ctctttgccg ggtttctcgt cggcttccta tggtttttcc tcagtttcgc
     8041 agccatgttc aacggcgtgt tctttctcct gctgctggtg cctcccgcat atgcgctctc
     8101 gggcgcgctt ctcgcgtggt ttttccggct tcctccggtt catgcggcct gttctcttgt
     8161 gggatcggcg ttaccctggg tggcctggct gtggccggcg accatgtcga gctcaggctg
     8221 gatgaaatct ctcgtgtggc ctgttttcgt gggcacggcc tttggcatga gcctccttgg
     8281 agcgcggctc gctgctgcca ggcgtcgtcg taagccgacc cagtctgacc ccgcgcttgc
     8341 ctagctggtt cattgctgaa ccgccggccg cgcagatctc attggctgcc aggaatgatc
     8401 gaaaatgtgc ggccggtggg tggctcgcga tcaggcgttc tctttttcca tggaggcaat
     8461 aaaggtatcc gcttcgtgga tcgacgcgtt gagatccttg atcagcaagt cgatgttgcc
     8521 ttcgaccgtg gtcaattcac tcttcagcga ggcgatggcc tgcgcattga ggttgtgctt
     8581 caagaacagg acatggtcct tgagtttggc caacacggga tccatcttgg cctccgcgcg
     8641 tttcatggcc ttgatcaatt gcccgtactg ggtgcgcgtc tgcgcgagct gcttctggct
     8701 tttctgacgc agcgatgcgt tcgaatactg cttcagttcc ccctcccact ccgtgaagag
     8761 ggcgtccgac acatcctcca ctgaagcgat gcggctgcga acggcttccg cgcgtttttc
     8821 gctctgctgg tattcgctgt tcaacacctc gtacttgtcc tgcaactcac cgccttggat
     8881 attcaacgtt ttcgtgaagc gatcgagcgc cgatttgaat tgttccttcg cgtcctgctg
     8941 ggcatcgcgg gcttttttca catcactgac catcaggtca cgtttgtgat agcccagctt
     9001 ttccatcgtt tcgtaatacg cggtctgaca gccggagccg gtgaggctca cgaggacgag
     9061 accacaggtc agagtgatcg acgcacatgc gtgagtgatt gaacgatgcc gaacgttcat
     9121 gatgccttcc ttttcaatgc aggcggtcca tgttagcgat agcccagctg cccggtatcg
     9181 tcatccccgt tgctctgctt cctctcgatg accgtgaagc gagctgatgc cttgtcgttt
     9241 actgtaacag accacgggcg cttgttccat gcgtgcttat gccgagatct cgtctagaca
     9301 aaaatgcgtg actcctggca tcctgcatag cgcgatcgct aatcatgctt ctcgccgacc
     9361 gagaggctct tcctttgaaa ggagcccgga ccatgccgac caataccgtc cgtcttcacc
     9421 gagtacttcg ctcgacgccg gaacgaatat accgtgcctt tcttgatgcg gacgccatgg
     9481 ccaaatggct gccaccaaac gggttcaccg gaaacgtgca tcatatggac gccaaggtgg
     9541 gcggaacctt caagatgtcg ttcacgaact tcaccacggg aaagagccac tccttcggcg
     9601 ggaaatatct ggaactcacg ccgtacgagc gaatccgcta cacggatgtg ttcgacgatc
     9661 cgaatctgcc tggtgagatg cacgtgacga ttaccttgaa gcaaatgtcg tgcggcaccg
     9721 agatgaccgc cgtgcaggag gggattccgg agatgattcc ggtggaagcc tgttatcttg
     9781 gctggcagga atcgctgacc ctgctcacga aactggtcga ggcggaaatt cccgactagc
     9841 ccatcacgcg tccacaggaa ggtcgtccga cggtacgtcg cgaatctgaa tcgagtccac
     9901 ccgttgctgc gccaggacgt ccgagataat gacgaccttg tctccggcct ggaatcgttc
     9961 acgatccttg aggatccgga acgccgtttg gagcgttttc tcaggctctg aactgaagtc
    10021 gattttgaag ggaaagacgc cgcgattcag caacatcgtc cgccggggct ggctcatgtt
    10081 cgtgaacgca tagatgttcg tgacaaacgg gcggcagttg gcgacgagat cggccatgat
    10141 cccgcgcctg gtaatcacga cgatgccttt ggccttgaca ccctctgcca gttgcacagc
    10201 cgctgcggcc agctgctgtt tgttgccgac atcgcgcaag cgtttggcaa actgtaatcc
    10261 cgggatcgtt tcagacttga gcgcaatcct gcggaggtac tcgacgcact tcaccgggta
    10321 ttttcccacg gtcgtttcgc cggagagcat gacggcatcg gcctcttcgt agatcgcatt
    10381 ggccacatcg gtgacttcgg cgcgagtggg atgcggatgg tgaatcatgg attcgaggag
    10441 atgcgtggcc acaatcacgc gcttgccgta ctcggcgcag agtcgcacga tcgtgcgttg
    10501 cacattgggc aaatcttcca gattgatctc cacgcccaga tctccgcggg cgaccatgat
    10561 gccgtccgat tccttgatga tggattcgag attcttgact ccttcctgat cctcgatctt
    10621 ggcgatgatt tttacccggc cgaccttgtc gcccatgagc gctttcagtt gctggatatc
    10681 tccggcctcc cgcacgaagg aaagcgcgat gaagtccaca tcccgttcca ggccgaagag
    10741 aatatccttc gtgtcttttg gtgtgatcgc cggaaggttg acgcgcacgc ccgggagatt
    10801 cacgtggcgt ttgctcttca gcagccctcc gtcgagtacg cggcagcgca tatgacgttc
    10861 atgcttctcc aggacctcga aattaatcag cccgttgtcg acggtgatcc ggtccccgac
    10921 gttgaccgcg tcgagcaagt cggcatagtt gatgtggatt gagctttcct cgacgttcaa
    10981 cggcccgcga gtcgtcacgc tgacgatttc gccctgccgc agattcaatt cattcgagag
    11041 atctccggtg cggatctccg ggccttgcgt atccagaaga atggggatgg gaaacttcac
    11101 tttcctgttc aggctcttga tgtgctggat gaccttagcg tgcgattcgt ggtcgccgtg
    11161 ggacatattg agacgggcga tactcatgcc ggcgtcatag agcttctgca gcatctcata
    11221 ggaatcggtc gcaggtccga tcgtgcagat gatgcgggtc ttttgcatgg tgctctccac
    11281 gagagatgaa gacatacgat cactgccttc gatacggcat gatcagcagg cggcagtgat
    11341 tggatatcgc gggaatcagt tggacgaggc cattctactt tttctcgcga ggctcgtcca
    11401 cgatcactgg ctcatcctgt cgcagaacga cacagtcggt tgctcagtgg atggtcccgg
    11461 tctgagtcgg gtggctggcg tcatcgggga ggacgcttcc tgaattcacg gtgtgcttga
    11521 tcctagcccg gctctggtat atgctccgct catcgagtgt ctctcgggac gtgacggatc
    11581 aagcatgacc atcaatcggc agggcattgg tattcgcatg gcggcggcga tgatgatcgc
    11641 cagctccgcc tgagcctgcc tgccaccgga cgtgtaccta tgcagcagag gcccctggcg
    11701 gattcactcc cgtcaggggc ttttttgtga tcgcaatcag ttgaggcagt gaaaggagag
    11761 cagggcatgg tgacagtgca gtcgatagca gccgcggcct cgcgcatcgc atcttccatc
    11821 tacgaatctc ccctggtcca ttccaggacc ttgtcccggc ttaccggcaa tacgatctat
    11881 ctcaagctcg aaaatctcca aatgacgggg tcgttcaagg aacggggcgc cctcaatcgg
    11941 attctcatga tgaccgagga ggagcgccgc cgcggggtca tcgcggcgtc ggccggcaac
    12001 cacgcccagg gcgtggccta tcacgcggtg caacgaaaca ttcctgtcca gatttggatg
    12061 ccgcggtcga cgcccctggt gaaactctcg gcgacacgga atcatggcgc ggatgtcgtg
    12121 ctgcacggag agaattacga cgaggcgtgc cgggcggcga tggagcgaag tgaggagcgg
    12181 cacgccacct tcatccatcc cttcgatgac gacgaggtga tcgccggaca ggggacgctg
    12241 ggcttggaac tgttgcggca gaatccaacc ttggatgtga tcgtggtgcc ggtaggaggc
    12301 ggagggctga tcgggggcgt cggctgcgcg gtgaaggaac aggcgagcaa tgtggaagtg
    12361 gtgggcgtgc aaacagctcg tctcccctcg atgaaggccg cgctggggag cagcattccg
    12421 gtcgaactcc cggccagccc gaccctggcg gacgggatcg cggtgagaac cgcaggctcc
    12481 cggacgctgc cgttggtgca acgctacgtc gaccagatgg tcaccgtcga tgaagatgaa
    12541 atcgccgccg cgatcttgag gctcctcgaa ggagagaaga ccgttgcaga gggggcaggg
    12601 gcggtggcct tggcggcatt gttacagggt aatacggggc accgtggcaa aaacatcgcg
    12661 gtgctcgtgt ccggcgggaa tctggatgtg aatcttctgg cccgcatcat cgagcgaggg
    12721 atggtcaggg atggaaggcg gttgcggctg cgcgtgctgc tgccggacta tcccggttcg
    12781 cttgaaggac tcacctccgt aatcgccaag gccagcgcga atatcgtgga gacatcgtac
    12841 aaccgtgccc attatggtgt cggactgaac gaggcggcgt tggacgtgac catggaaaca
    12901 cgtggccgcg aacatgcgtc ggagctactg gcggcgttga gcggctcccg ttacgagttc
    12961 agcgtgatcg aatagtcgac gggatgaccg ttgaattgat ctgatgctca attcacggcg
    13021 ccgcgcgccg cgtcccttcc tagcgagcaa tgggctgcgg ttgcctggct tctgccaatt
    13081 ggacattgac gcggaccacg cccccattca ggttgcgata gggatagcgc tccgtgatca
    13141 gtggcaggtt atccgcgaca tcctgggtcg tcacaaccga gagcgccggc acgtaccgtc
    13201 ctgaaggcac cttcaccccg ataattttgg cacccggttc gacgacgaca cgatctccca
    13261 cctccgcacg gaacaccaac gcctgcatgc cgatgaaggt atcattgccg actcgagcgg
    13321 gaccatgcac ttgcgactga tgcgcaagag acacacggtt gccgatgtag acggaatact
    13381 tcatgccgtc gacttcgact tcgttctccg gaagttcatg gtcgccttcg aaggtctcga
    13441 ggccgtgaac cacgacgccg tcctgcacat tgctctcgtt tccgatgtgg atgtgccgcc
    13501 cctcatcacc gcgcaccgac gcggcgggcg cgacaaacac gcgatcgccg agggtgacgg
    13561 atccgatgac aatgccgagg ggatgaatcc tggtgtgatt cccgatcacc ggcgcttcga
    13621 catccggcat gaatgtcgtt ttcacgctgg gaccgacgta gccggtaagg atgaaaccga
    13681 gcagaatcag gttcagacag agggacagtt tcaggttgtg cgacatagct ccaactttct
    13741 ttcgaacgaa agcctcgtca tgcagtggtc tgtctgagaa gactctaggt attccatagg
    13801 tgaaagtgta ggcgtgggac cctgaatgta caaggaaatg aaggagattt gaaagagcag
    13861 gaagtctgca ggctgtgccg gcaggcgttc tagatgctgt gtggtgggct cagatatctc
    13921 agtggctgtc tttctcgcga atctgcatgg ggaccgtggt ggcctgttgt gtcagatcgc
    13981 gatggtgcgg agcaagttgg gagagggtga tgtcgtgggt gtcgtgcacc gaaaagcggt
    14041 aatctttccc gtgagtcagc tgcggtggca cctcgacaag caattgcccg atatagacca
    14101 cgcggcctga ttggctttcg aagacgagat tgaggtcgcc tcccgtgccg gccatgcccg
    14161 tgcgataaaa attcttgatc agataccgtc cgggtcgcaa ccgggccaga aacggccggt
    14221 ccaggcccgc cgtggcgtcc acgtgatatc gggctgcata gggactttcc ccattaggat
    14281 caccaggctg aacctgcacc acttcaacct caaacatcgt ggactgatcg cgtaccgccg
    14341 tgtcggctcc ggagcctttc tgttcttgcc tgaccagcac cgacccgata acgattcccc
    14401 attcaggacc cggtgccgcg atctgttcgt gtgacatgtc tagtgatgac gcgcaggagc
    14461 tgaggctcca ggccagagcc gcaagcagaa tggaagcaac aggtttcggg agagaagtac
    14521 agtcagggcg catgcatcct cactctcttc ggttgggctc tctcagtcaa gacgggcgct
    14581 cgtccgttgt ctgcgtgggt acgcgcgccg acgaccacgc ggctcgtgct catcgtgcca
    14641 tgcgaccggc ccgcagattc gccggaacgg caatgtcgat cttcgcgggc cttggttgtt
    14701 tccgcctgtt catcagctcg atgaacgtgt ctttgtcgat tgagagattg agccgttcat
    14761 tgaagcgttt ttcttcgccg atggtggaag cgacccggcc ttgatagtcg tggcctggat
    14821 agacgatggt gtcgtccggc agtgcgaaca gtttctgtcg aaccgagtcg aacagcgtgg
    14881 ccgctgatcc cccttggaga tccgttcgac cattcccccg aatgaacagc gcatcgcctg
    14941 taaacaccgc acccggtaag agataactgg tgcacccgtc cgtgtgaccg ggcgtcgcca
    15001 gcgccgtcaa ggcactcccg ccgaaccgga gtgtctgtcc gtcagaaaga aacaggtcgg
    15061 ctcccatgac agttccggct gcgccaccac catagacaat ttgcgcgccg gtgcgatcct
    15121 tgaggagcga cgcggcggtg atgtggtcgg catgcacatg ggtttcgatc gcataggcca
    15181 gcgtcaggcc cagttccttc aggatccgga catcgcgttc gatgttctcg agcacggcat
    15241 cgatgatcac ggcctggctt gagctgtgat ccgctacgag ataactgcga ctttccgccc
    15301 gatcgtcaac gtcttgcgcc tcattcacgt ccagcagaat ggtgacggta tacgtctggc
    15361 tcgctgccat ggatgaactc cgagataaaa ggaagagcga cccgaagaaa ccggcgcatt
    15421 gtagccggtt gcatggcacc ggtccatggc tcctggcaat tcgatcaggc gtgtgagtga
    15481 ctgagggctg ctggccccat tgggaagatg tggctctccc tgggttctcg aaagaggcct
    15541 tcccctcata cggccaggga atcgtagacg tagatgcttc ccacgtcgag cacgtcagcg
    15601 ggtctcattg ctgcagagac gaatgccagc atctggtcgt tgaggcggtg tccgcggaga
    15661 ctggcaggcg cggattgcgt cggaacgtag gcgcgagtat gccgggccat ccaggcttcc
    15721 cacaggcggt cttcattgca atgattcaga tagaagacgg gatcgttggg ggaactggac
    15781 ggcaacatat ctccgccgac ccacacgtgc acgcggttat gcagcgccgg tgcgtcaggc
    15841 gctgcccagc cttcggctcg attccgaaag ccggccgatg ttgtatccca gttgggttga
    15901 tcgtagagag tgagcgcaag ggcgccggcc gtatcagtgc gagtggggag ggtcgcgata
    15961 ttcaccccca gatcccgccg caagccgcga ttgctctgga tcagtcggcc gtttgcatca
    16021 gcctcgaccc gaatgcgaaa gctgtcagga tcgtcggcga caaagcgaaa gggaccggtc
    16081 cgaaccggtg agcctgttcc gccgacaccc gatgcccgcc aaagcgcaga ttgtcgctgc
    16141 tgacccggtg tcctggatcc atccgcggcc caatcccagt agggaagtcc gaacgtatca
    16201 tcgcggagca ctcgttggag gtttcgttcc aactcgatca acatgaaccg gtgccagggg
    16261 aagaacacgg ggccgcgatg ggcggcattg cggtctccct gtgacggagg cgtcatagtc
    16321 gacatggccg tatggtgcca gacgacgaat aaatcatagg tgctgatctg tcgagcggga
    16381 ccggggatac cgagactgct ggtcgtcgga cccgtgaact cacgtttcaa gaggttcacc
    16441 ccgcgggcgt actgcgcacg aacgttggca ttggtgagaa tgttgcgtct ggtcacggcc
    16501 atgagttcac ctcagtggtg tgttcggttc gtgtggcgaa tgcgtcccgg tcccttgtgc
    16561 ggacgtttct tcgcctcgaa ctggtggggg aacacatcga tgatggcttt caccatgtcc
    16621 agcgcggagg cgaacgacaa attcgggatg gggtgataat gaacgtgccc gtcgttggag
    16681 acgccaaggt gaccgagcac cttgcggccg tcgacggaaa tctcgtaccg ggtcttcacc
    16741 acgatgtggt ggccttgatg gtccgcctcg cggatcgtct cagtggcggc atgatgccgc
    16801 cgctggcttt gtttcactcg cggggcatgc cgcttcacat acgacgcgat ggactggggc
    16861 gtcggcaccg gctcgcgttt tctattggcc ataagttcac tccttcctct cacctatcca
    16921 gatggctgaa cgatatcagt gtacggcggt agctcgcgct ggtccagagg gcctgcgggt
    16981 gcccgcttcg taggggacgg accgtcagtc acgcgcccgt tcgggcctga gaatcggccg
    17041 tgcctgttgg aacagaaacc aaccgtagag caacaacagc actgtgaagt tcgggatgag
    17101 aatggccggc tggctccatt gccacccatc gaatttccaa tcggagagag gccagaaaat
    17161 gggggttgga aaaaacgcaa acgaatgggt cgggatgtcg actaagacat gtaaacccca
    17221 ggcaccaagc tcccacaccg gtcgtttcat gacaagccac aacagggcaa agacggcgag
    17281 gaagacgacg aagctatgtg tgacatcgta aagatgatgc acgtagggcg gaatggtgga
    17341 ctccggtggc gtgccgtggc tgaaatcagg cttcggcgac aggccaagcg tcgcagctgc
    17401 ccacaagatg ccgaacgaaa acaggtccgg cgccaggccg attccgaacg ccagccagaa
    17461 gctcgtgcga cttcttcgac caaacgccaa cgctccccac agaccgtgcg acacgatatc
    17521 catgtcgaga cccctcctgg gttcaaagca actggctcat tataccgggc gtgctggcga
    17581 gaggcgtacc tgggcaccat cccagtatgg ggatgcgtaa cgagggggca caatgcgtac
    17641 tcacgccagc cggaggagtt gccatggatc aaaattttaa agtgggcgat catgtgagtt
    17701 ggaattcgga agcggggcgt attcgaggga caattcagaa aaggatcacg gctcccatga
    17761 agttcaagtc gtatatcgcc cgagcctcca aggagcagcc gcaatatctc gtgaaaagcg
    17821 acaaaacagg ccatctggcg atgcacaagg ggaccgcact cagaaaaatc ggcgggcaat
    17881 ccgcttagac gaaacggtcc cggtctgtcg cgctggggat gcggcaggta tcagccttcc
    17941 ccataagccg gtagcgatgg cgtgcgacgt agttgtacag aatatctcgc agtccgcggg
    18001 gtataagaat aaagccatac agcaacggcc accagccaga cagcgttctg gcgattctca
    18061 aagcggcggt cgatttggtg aacacctgac cccgctccag caggagaaac gtctcgaaat
    18121 cctggacagg caatcccaac agcgtgagca gttcctgccc cttcgccgat tgcaacgtcg
    18181 cgaagtgaaa gcgcccacgg tcacgagcca tcgtgaaatt aacccaggca ttgcaccaat
    18241 tgcagatgcc gtcgaagaca atgaggcgct caaacctcgc ccattcatgt ctggtgggat
    18301 cgatcgtcgt catcgtctgc ccgggttgac ccttcctcgg caatattgtg agttcccgct
    18361 ggaaagaatt ccactgttga atcgcggatg actggattac atcgcgcgtg ctcgttcgct
    18421 ataatgcgcc cgaacttttt aagggaggcc tgttatggca ttcaatcaag tgggagattt
    18481 ctggctggcg cccggtgaag ccatccgcgt gcatatcgcg cgtgggaagc tggtccctga
    18541 tgaggagtgg ggaggggaag acttcggcgc ccagtggatc atggcggacg gggtcgggat
    18601 caacccggtc cgtctcatgg tcagcgaaca cacgaaagaa aaaaaggccc ttcgccggca
    18661 tcccggtccg tcaggtcctg tcgtctattc ggtgaccgtg accaacatcg gcgaggagct
    18721 ggcacatttt acggttcagg gcggcgggaa cgtctaagct ttccagagga gattgcacgt
    18781 gaaaacaaaa cgaactatgc atgtgctgac gaacaaaaag ggggcgatcg tggggggagg
    18841 cctactgact cccggaaaag atcataaggg caaaccggtg catattcaga tcgagccgat
    18901 gaagggacag tcgttgaagg aagtggcggt cccggatgag ctcgctcggc tggaaggcgc
    18961 agacttcttc agccgcttga tgtgtgactt ccatctgcca cgcggaaaaa aggaactggt
    19021 gcgaagagga agcaaaaggt agatcggggg acgatttcca gagtcgatgc tccaacggcg
    19081 gtgacgacct acgccaccat cggcggttct ggtttcatca cgggtactct gttgaacgca
    19141 cagcgtttct tcagcgcatg tcggctgagt gtcactgtca ttgtctcagc gagaggcgta
    19201 ttgctgggaa gctaagtggc tgccggattt cgcgagcttc cggtgacatt tcccgtggcc
    19261 ttcgtggccg cgcggctgcc gtcttggtcc tgtcgtcgca attttcgccc tctgcttgta
    19321 cctcggggca acctgaggta gggtcgaagg cagatcatga ccctcctgca gcccaacact
    19381 ccctggtggc aatcggccat cgtctatcag atctatccct ggtcctttca ggattcgaac
    19441 ggggatggaa tcggcgatct tccgggcatc atcagccggc tcgactatct caacgggtcg
    19501 cccgactcgc tcggcgtcga cgccatctgg ctatctccga tttatccttc gcccatgcat
    19561 gacttcggtt atgacgtcag tgactactgc gatatcgatc cgcgcttcgg cacgctggca
    19621 gacttcgatc gcctcgtctc ggaagctcac cgccggggca tacgagtcgt catggatctg
    19681 gtcctgaacc atacgtcgaa ccagcatccc tggttcatcg aatcgcgttc gtccagaacc
    19741 tccgcgaaac gcgactggta ctactgggcc gacggcaaat ccggccggcg gcctcccaat
    19801 aattgggctg cgcgattcgg tggatcggca tggacacatg atccgcacac cggccagtac
    19861 tacttacatt cgtttttgtc ggagcagccg gacctgaact ggaccaaccc tgccgtccgc
    19921 gacgcggtct tcggtgtcgt ccggttctgg ctcgaccgcg gggtcgacgg cttccggttg
    19981 gatgcgatca attggttggg gaaagacacc agctggccgg acaatccggc ccgtctggcg
    20041 tggcgcagct attatcgaca aatccatcgc tacgaccgtg accagccgca tacccatgag
    20101 gcgctgcggg cactccggtc ctcactcaag gatcgagagg acattctgct ggtcggggag
    20161 acgtcctcgg atactcctgg tgggccggcg gcattttacg ggaatggatc gaacgaactg
    20221 cacgaggtgt tcgactttcg cttgcttcgg tctgattggc atccggacac ctttcgccgg
    20281 ctcatcctgg acagcgatcg cgcggtccca cagggtggct ggcctccggt ggtgttcagc
    20341 aaccatgacc agtcgcggca catcgatcgg tacggcgcgg gcggcgaggc tatccggcga
    20401 gccagggcga cagccctgct attgttcacg cttcgcggta cgccattcgt ctattacgga
    20461 gaagaattgg gtcttcatga tggcaggctc aggcgagcgg acctgcacga tccctacacc
    20521 atccgctatt ggccgtggaa aaccggacgc gatcccgcgc gaactcccat gccctgggac
    20581 gacagccctc aagccggttt cacgaccggc agaccctggt tgccactttc acaagcatgg
    20641 cggcagacga atgtggctgg ggagcaacgc gatcctggtt cgatcttgtc cctgtacaaa
    20701 cggcttatcc ggatgaagaa aggatcgaac gccctcaccg tcggagccta tcatcctgtc
    20761 gatggagggc caccggaatg cctgttcttc acacgagaga gcctcgtgga tggccagcga
    20821 gacgctctgt ttatcgccgt caacttcagc gcccacacgc agaagatctc acttccggct
    20881 ggcccttcca agctcggacg tcacggcacg ttgatgctct ccaccgaccc cctgagagcc
    20941 gaagggccct ggacggcaga ccattttctt ctcggtcccg acgaagggat tgtcgtcagc
    21001 atgcagtgac caagcaccca ccgacccacc gaccgtttct tcggcacacg tccattcaaa
    21061 tagggtactg taagggatgc ggattgcgac ctggaacgtc aactcactca aggcgcgtct
    21121 cgagaaggtc ctgtggtggc tggatcgggc caagcccgac gtgttgctga tgcaggaaac
    21181 gaagttgtcg gatgccgatg cgccgatcga cgcgttcgca caggcgggat acgcgctggc
    21241 gcaccacggt gaaggccgat ggaacggcgt cgcgatcgcc agccgctgtg gcatcagcgg
    21301 cgtcatcagc aatttcggag agccgttgca gccggcacgt tcgactgaca cgggcgacga
    21361 cgagccgttc gccgaagcca ggatgttggc tgccgattgc ggaggactgc tcattatctg
    21421 cctgtatgca ccgaatggcc gtgaggtcga ttctccgttt tatgaggcga agctggtctg
    21481 gttcgacaag ctggcgcgat ggctcaagca gacgggtgca ccgcagaaag cctgcgtcat
    21541 cgggggcgat ttcaacgtgg cgcctcagga catcgacgtc tgggacccgg ccgcctgcca
    21601 cggcggcacg cacgtgtcag atcgtgagcg gcgtgcattc gaccggatcg tacgcgaggg
    21661 attcgtcgat gcgtaccgat gccaccaccc ggaaccgggg cggtacacgt ggtgggacta
    21721 ccgcgccggc aatttccaca agaacatcgg catgcgcatc gatcacctgc tcgtgagcac
    21781 tccgctgaga gatcgtctga tctggtccga aatcgatcgc gaggcgcgga agggaaagcc
    21841 gttgccttcg gatcatgctc cgctggtcat tgatctggat acagccggcc atcctttcga
    21901 tgccggctgg gtatcggctg agtcacgcat tgcggcgcga actggaaaaa aacgatgacc
    21961 aggagccggc aaggtgatcg gctcaatggt tctgcctagc ggttgatcgg tggagacttc
    22021 tcttggccgc ataggctccc atctttgcgg tgacgatcga cggtagggca aggacgccga
    22081 acatcatgat tccgagcggc aacacagtac ccgggccttg agtcagcagc atcacaagcg
    22141 cctgcccgac aagaggcgcc gctccccagc gccaggccct gttcggttca aggtagccaa
    22201 gcagagcaga cagcaggcag accgcgggca ggcccagcgt gaaatacaga tcggaatccc
    22261 acgcctctct cttgtgagcg aaggccgaga ccaacaacca cacgacgatt ccgccagtca
    22321 ccgccgtcac gacaagccag gtatcccgtt tcatgacttt cctctccaaa ccaacccgat
    22381 cagccgcgtg ttggtgttgt aaggtcaggc tagcggtcct gcatggacgc agcaagtatt
    22441 ttgcgcattc gagctacgcg gtgttcttct caagagcaac aatgtgagga gggaggaaag
    22501 aagtcttatt cagagacggg actaacgcag tcttcccgac aggcgcgaat caatcgagga
    22561 atgggcctcg taccagtcat cccgtgttaa cggcagacca gccgatccct cctgcgtttt
    22621 cgacagcaag gtttgataga ggttgagctt gcctgtggca aagccatggg cggcgctggc
    22681 gagatacagg cgccacaatc tccatgtgga ttcatccatg ctggacgatc cgggcgtcac
    22741 gttcttctcc aggcgctgtc tccattgtcg aagggtcagg acgtaatgct ctcgtaaaca
    22801 ctccacatct cttacttcaa agccggcggt ttcagcgaat ccggtggtgg tcgagatggt
    22861 ggtcaattcc gcatcaggga agacataggt attcacgaat ggcccgaatg cgcagcgttc
    22921 cccatgccga atcccgatcc catgattgag aaaggttccg cccggcttca gcaaattcca
    22981 gatatggcga aaatagcctg agaggtgagt ggctcccaca tgttcgacca taccgacgct
    23041 gacaatcttg tcatagcgat tgggagtgct cagttgtcga tagtcgcgga gttcgacctg
    23101 acagcgatcc atgagtccat gttgttgaat gcaggcctct gcaaattcag cctgcttctc
    23161 gctcaaagta atgccgcagg cctggacccc gtagtatcgg gcagcatgaa cgatcagtgc
    23221 gccccagccg cacccgacat cgagcagccg ctcaccacgt cggagacgga gctttctgca
    23281 gagataatcg agtttgtgta cctgcgcggc atcgagatcg tcggtgaaag agaagtaggc
    23341 gcatgaatag accatctgcg gatccaacca cccggcataa aattccgggg gatgatcgta
    23401 atgatacgcg atggcttgtt tgtcgcgaag gagtgaatgg ttgggcccgg tgacgtgtgc
    23461 ggccaggcca ttcgtggtga aggaggggtt cgatggccaa cgtaatgatt tgaaccccaa
    23521 tcgcagccaa ctcccccagg tcacgggatg acgcgagagg tattccgcaa gagggatggc
    23581 ttgttcaaga tctccatcga tctccagatc accggagata aacgcctcgg ctagtgcgag
    23641 ctcgctggcc ttccacagca tgtccctcag gacggttggg gtgcggagga tgagggtgaa
    23701 ttcgggagtc gcgccgggat cggcaggcca gattgtcccg tcccacaaac ggatatcaaa
    23761 attccggggg tgatactgcg ccagcaactc tccaagcagg ccggtactat cgtgaatcgt
    23821 tttcctggag agagtcatcg agttgtctat cccattcacc ggcaggtccg ttcgttcact
    23881 gaggctcgtg cagcgctgag ggtcagccca atcggaggct cagcgtcatg ccgtcacaag
    23941 ttctcctcac cgcaatcgta ctgccagcag gattctagtc tactgggcgg agccctagcg
    24001 cgttcccgtg gataatccaa acatcatgta gccataattc taaggcaatc agcggctcgt
    24061 gcggcgtttc ttccaaatca gcaggatagc gagaatggtg aggggaaaca ggagcagcag
    24121 tccgatcgag aggctgtaga cgggcgcgcc caagagtccg gagttgcggg aatcgtgata
    24181 gtccactccg cctgctgcga tgaaggaagc gactatcaca ccgatgcatg aggcgacgat
    24241 cacacggacg agccaccacc agcctgtgtc tgccggcaac aaggccgtcg cgaaggtgag
    24301 gggaatgccg accaatggaa tggctaccca gagcgtttca aggaagtcgt tccccgccgg
    24361 cgtcgacggt tgattcattg cggcggcgac atacaccgcg agactgatca tcaaccacag
    24421 tccttcaggc cccagccata cccgggacca gcgtgccatc accacgtcgg tgcgtgagct
    24481 ggttgtactg cgcgccgttt tccgacaggg ccgcgatcca tcgagtgttt cgccagcctc
    24541 attgcgcctg ggcccgccgt gacagggaca cgacatacga ggcggaacga gcggcaatca
    24601 tcggatcggc tgcttcgata ccggggacca acgctcccgg catgaaaagc agcttcttct
    24661 cggcagcctg actatccgcc accgctttgg tgatgctgat cgtgcccaac ttgatctgtt
    24721 tccggctatc cggccaggcg atcgagggat cgtcgatctt gtcgcccttt tccgccactt
    24781 gcaggaggag cgtgaacttg gcagggccac ggcgcaatct ctcgcgaatc tccgcgctca
    24841 gataatccgg gtccatcgtg ttgagttgtt cctgggtgag aaagtgctcg cccgccatgg
    24901 ggcgaagctg gtatcgaccg aatgtgacgt gccctttcgc attcgtgaat ttgaacgcgt
    24961 tgatgccgaa atagggcagg gtggcataac tgaccggttg aggcttcggg ctctccacga
    25021 aatgtttggc cgccggatgc gtcgctagga actgctcgat gggagtgggt ttaggggcat
    25081 cgggtccgct tgccccgacc gcgaggagaa agtctctgaa ctcttccgcc gtggccacag
    25141 gaaatccgtt aaaagaaagg acgacgagat ccgtcttgga tccgtcgggc agggaaaatt
    25201 tgacggccat cccgcgaggc atctggctgg tgtccggaac ggacggttgc cccgaaccgg
    25261 cagagaaccg caccgtcacc ggaacagcgg ttttcttctt ttgcaggtga gccgccttgc
    25321 tgacggaggc cgccgatgcg gtgggcgtga acgtgccttc caggaccatc ccttttggat
    25381 gattggcccg catacctgga tgcactccga agaccccgtt gaaggcatcg acgagctgct
    25441 cggccaggga ttttggattg gcgggaggtt cttccgcaag gactgaaagc gggcttatca
    25501 gcgccacggc gagtgcagca gttgctatac gtgatctcca cataggccat ttcctcctga
    25561 ataatgaaag atccggctgg cagagcggca tgcacggtca cctactcaag cctgtgaaag
    25621 gtcggtcgga agagcgatga aaccctactc ctgcgtacca atcgagtcaa tggagcaatc
    25681 cagaccctga gatgcgctgg cgtccgcccc gacgtatctg aggtcgatca ctccgatgga
    25741 tgaactccta caagggatgg ttcaggtctc ctcagcaaca tctgcgacgg cgttcgccgg
    25801 ttctcgcgat tccaggggta ttaccagtcg gccttacgta aagaatgcgt agaatagcgt
    25861 cggatcgatg gcgggttcgg cctacctttg cgacctgttc tcaatccacg cagagacctc
    25921 cacacacaga cgccagcacg caatcggcat accgacacaa cgagagaaag gaactctcat
    25981 gatggccttc tcgctcaaac cacttcgtca gcaaatcatc gtcatcaccg gggcgacatc
    26041 cggcatcggc ctgaccaccg cgcggatggc ggctcgacga ggggcgcgac tcgttctggc
    26101 cggtcgaaca gaaggagcgc tgactgaact cgagcgggaa ttgtcacagg gacagcatcg
    26161 ggccgtgacc gtgacggcag acgtggggaa ggccgccgat gtgcaacgga tatcacgtgt
    26221 tgcgcgacag atgttcggag ggttcgacac ctggatcaat aacgcgggcg tgtcgatgta
    26281 cggacggctc ctggatgaac cgatcgaaga tatgcggcga ttgttcgaga ccaatttctg
    26341 gggattggtg tacgggtcac tggaagcggc gcggtacttc aaagcgcacg atcaggccgg
    26401 ggcgatcatt aacgtcggca gtaccctctc agaccgcgcc ctggtgttgc aggggatgta
    26461 ttcggcctcc aagcatgccg tgaaagcatt taccgatgcg ctccgcatgg aagtggagat
    26521 ggatggtatt ccgatctccg tcaccctcat tcaaccgggc gccattgcga cgccctactc
    26581 acaacatgcc aaaaattaca tgaagacgga gcccacgaat ccgccgcccg tgtattcacc
    26641 cgagacggct gcccagacca ttctgtactg cgccgaaaac agagagcgcc atgtctacgt
    26701 cggggccagc gggaaaggca tcggcatgat gggctactac gcacccaggc tggctgacct
    26761 gctgatggaa acattgtttg cccgacaaca acagtcggat cgtccccccg ccaggcctcg
    26821 ccgggacaac gccttggacc gcccgtccgg ggatttgcgg gagcgaggcg actatcaggg
    26881 ccatgtgtcg gaatcgagtc tctacaccac cgcatccctg catcccatgc tgacaggggc
    26941 cgcactcgtc ggcgcgggac tcgcgctgaa gaccttgtgg ccatcgaaac atacctcgga
    27001 gcgagtgcag aacgcaccgc gcgcgcagcg acgttgatcc ttcgtcagag tttgcgctga
    27061 ttcatccctt ctgcttccca tccagcaggc aactcacatt catgtcgccc atgacattga
    27121 tcgccgtccg gcagcgatcg agaaaccaat ccaccgtgag caggacggga ataaactgga
    27181 cggggaggcc gacggccgtg aagaccatcg tcatggtgac aagacctgcc tcgggaatac
    27241 cggctgcgcc gaccgaggca atgatgctcg tcaggacgat catcacttgt tggtgcatgc
    27301 tcagatcgat cccgatcatt tgcgcaataa acagggctgc catggcctcg taaagcgcgg
    27361 tgccgtcgtt gttgaaattt gccccgacca gtgcgcccat gctggcggat tgttcgcgca
    27421 ggccgatgcg atctttgagc gccgcgtaag tcaccggcat cgtggccgtc gaactcgccg
    27481 tggaaaacgc catgaccagc gcatcccgtc cgccacgaag caactcaccc ggacggaccc
    27541 aggacccgaa ccgaacgcga acgagataat aggcagcctg gatcgtgagc gcgagcaaca
    27601 cgctgaggac aaagataccc agcgcctgaa actgcgcgaa accctctgtt cctacaatgc
    27661 tcgccacgat gccgaacacc gccaggggca ccaccgcgat gatccagtgc aaaattttga
    27721 tcaaggactc cagcaccagt tcgacgagat gctcgaccgt tccgagcggg cgatggcgct
    27781 ccttacgcag agccatgccg aaggccaccg cgacgaagat gactccgatc acttttccat
    27841 cgtcaccaag cggccccagc aggctcttgg gcacattctc aataaacgcc acgagtggat
    27901 ttgccgtatg cgtggtttcg acggcgggtt ctgctggcgc aagtcccgca cctcgtccag
    27961 gctggatgac gttggcaacg gtcagcccga cagtgatcgc caccaacgta ttcatcagca
    28021 gcaatcgggg gaggcgtgcg gccagcgggc cgccgagatg cgtcgtcatg aaggtgtgga
    28081 caatcgcgac cagaatcagc ggcggcgcga gcgcccccaa aaggcggagc accagcttgc
    28141 ccggcgtggc tagaaaggcg gcctggtttc ccagcgcgag ccccgctgca aggcccagta
    28201 tcacgccaat gatgatgcgt ccatacagcg ggatctgctg ccatcgtgcc accacgcctg
    28261 tctgcgaagt gacgatggct gtgttctcta gggcattgtc atccacgaac gagtactccg
    28321 atgtgttcct gtttctcagg gaatgcgcag ttgtgttaac gggacaaggt tagaaggagc
    28381 cttgtcgatt gtggcggata ctatcagatc tgcttctgca gtggggagat ggatgcaaga
    28441 gtattcacgc cgcgttcagc tcgcactgca gccgtgggct tgcccgatcg atactgaatc
    28501 gcagccatga cttgatgcgt atagttgagc aggcgtggcg gatgatggac caccgtcagg
    28561 ccgggttgca gcggcaccaa ccccggcccg taggagtcac gccaggtcgg ggtagaaata
    28621 gtcggctccg acggggagtt taccgagcca ttgggtgaag cgtgaaacct cgtctccgcg
    28681 aaaaatgcat ccgtgctgag gaagaattgc cgtgatcggc agtggcgcga gttgtttgtt
    28741 catcccggcg acagctcgat tgctggacat atagcgctga tgaaatccct gcatggcttg
    28801 ggtatgcgcg ttccaatcat caatcacgag cctgaaggtg tggtccttgt acatggctgc
    28861 gccgacgtct ccggaaaaca ggaacccgct gaccgtatcg tgaacaagaa tattgcccgg
    28921 ggagtgcaga aatggcgcgg agagacaatc gagtcggccg ccgtcattca acgggatcga
    28981 aaggccctcg tccggtactt tgacgaaatt gaggttgtgg gccttcccgc tcaggtccct
    29041 ggactcataa tgcagcatga accgggtcca taagctcgac acaaccagct tgatgtcgtc
    29101 attccacttt gtccaactgg gaaatgaggc acagatatcg gggtcttgat ggctgaagaa
    29161 caggtgggtg atgctattcg ggctgaggag gagacacact ttcttgaaca catgcgggaa
    29221 acggtcgaac ccgcccggtt caaggatgta gccctcgtgt ccgtcgatga tcagataggt
    29281 gttgcactcg atctcgtctc ctccggtgtg tgtgccgatc caatagactt tgtggccacc
    29341 gttttcgaac agcaggatgg cgtcgtcgga gttgatcgaa tcatttttga gattggtcag
    29401 cggaacagac accatggcaa cctccatccg gtacatcact acacggcccg gcctccgcac
    29461 ccctaagtga aattatagag gaacatccac tgatcacaaa cggaatacgc gaatgagggg
    29521 agcctgaagg ggaagttctt ggagaaaaaa atggtgcctg gcccgttgtc tgttgcatgg
    29581 ggccaggtgt cgttgtgtat cagtatggta cgtgaaccgc tgacattctc ccaaggcagg
    29641 cgtgtgagta ggttacgcgc ctgcagtcgt tcactctcgc agacctgcgt gtcctagctt
    29701 ggactctacg tgttagagag gttcggcgcc ttctcggaat atgcatgagg cgcctgacaa
    29761 aagggttcgg ctgggcccct gttacgtatc caggtctgga tgattcctgg gaccatttcc
    29821 tggcgtcgtc ttggagcgag tggctaccgt tgttcgggtg ggacagcgtt gagcgtggcg
    29881 gcaaatgcgc gatgggcgga gggatcaacc ggccgatgcc ggatcgtcgt gctcgccggg
    29941 tccttgccga gatagttctc ccctctccaa ttcttcgacg gtctgatggg ccttggttcg
    30001 aagccgccgc cgcaattggg gcagacattg tgcaacacgt ccgtgacgca tgccgcgcaa
    30061 aacgtacatt cgtaggagca gatgcgcgcc tcgggtgaat cgggtggcaa cggtgtattg
    30121 cagtgctcgc aggttggccg aagttccagc atgatggatc tggttcgcta aggaatggtc
    30181 atgtgtccat tctacgcgct caagcctcga ggatctatgg gagagtaacg aggggagttc
    30241 ttgcctccag ccggcgttgg gccggaatcc ggttaccctc gcgagacggt tcggtacctc
    30301 gcacgtgaag ttcctctcct gaccggcaga cactctgtgg aaggtcttga gggaattcat
    30361 cgctggctcg actgacgcga tgggggccca aggtgaactg tctgccgcgc gccggaggga
    30421 cgccgcatca gctcgccttc atcgagggca gcctcgctgg tggggcatca tgctgggccc
    30481 ttggtagatt acgtgtgatc ctttctttgt cgctctgtgc gaggccaaat aaaactcgtg
    30541 gtgtgtctgc gccatccttg gttgagatag aacccctctt tgcccaccgc cgccgtcagg
    30601 gtcgtaaaca catagttggc aagtgagtgt tcgagatacg acaagatatt gctgccaatc
    30661 ccacggcctt gatactgagg gtcaaccacc aaatcgacga tcaatgcata gtattgtccg
    30721 tcatccaccg tacgtccaac accgaccaac gtacgctcat cgtaggcaca tcgaacatag
    30781 gagctggatc gaaaggcagc ctgaatgtct tcggccttgc gtttccccca accgacgcgt
    30841 tcgaagagct cggacatcct ctcccagtcc acaccctccg tgtcattctt gtactgtatg
    30901 tccatgggtt gttctcttgc tagggtgaga catgagccct ggtagcatga ctcgcgttca
    30961 cagtcttctg ctcttcgcgc cggtcagctg tccctcacgt ctctttgtct gtacccgttg
    31021 acggtcagga cgactccaca caccaacagt accgccgtca tggccaatac ggctgtggga
    31081 tggtgagcgg tctgttggga cgagactgga gcgaccatgc ggatcagccc accgaggagg
    31141 agtagccacc ctgtgagcgt gatccgcaca ggccagcgag gcatccaata attgtgggca
    31201 cggacaatag tcaagcccgc gacaacgggc aaagtgccgt tgaggtacgt gagatgcgcg
    31261 gacgtgttcg tgaagatctc gaggttcagc aactctgtaa cggagacgac aatgaggctc
    31321 ggcccgagca gtgccgcaac ctgcttcgac tgtgccacct caccctctgt tctccggcct
    31381 cagcgcgcca gcagtttcac aggacaggcc ccggcagcat tgtactgttt ccggtgggtt
    31441 tggtctattg cgtgacgcat gtggttggtg cgtgggcgat gggccggagg tgccaaggat
    31501 gaaagaagac ttgggccggc ttcagaaggg cactccttgg tgccagtccc tttcagtgca
    31561 tccagcaccg caaggccaga cccaaagagc caggtcgcag ccggaatggg taccaggctc
    31621 gggccgaatg attcctggca tcgtacgcct tccgtcagac atcgcccgga cgcgcccgtc
    31681 gaaagccttt acagccaatg gtcatttagc tagcctagcg cctcatgcag ggattcacac
    31741 cggcgttcgc ctattttcta atcgtcttcg gggctggatt tctcctcggg acaatccgcg
    31801 tcctgctcct ccttccggtc ctgggcgaac gggcagcgga actcttggag atgccattca
    31861 tgctcatcgt cattgtgctg gcggctcgtt ggatcgatcg gcgttttttg gcgggcgtgg
    31921 acgaccgggg gcggatcgtc gtgggactgc tggcgttcgg actggtcttg agtgcagagt
    31981 ttgtcgtggg catcgcgctt cgtggcatga cgccacggga tgtgctgttc gctagagatc
    32041 cggtttcggg tgcggcctac tacctcagcc tgctgctctt tgccgtcatg ccgtggttaa
    32101 tcgcgccacg ccgccggcgg taacggcagc gtggaggaag gagcaattcc tggcaacttt
    32161 gtttctgctt gattccagtc caaactgttt cgccatccgc cgcaacaggc ttcttcactg
    32221 ttcgggcggc cccgttgctc attgtttcag agtgattgca gctccgatgc ggtctgtggg
    32281 cgattcacac gcgtcaccca accgcctggc cgtgtcacga gtcagtcact gagccgagct
    32341 gtcagcttgg catctcctta gtcccgccac cgctcgctcg atcattgccg ctcttctgcg
    32401 cgagtcacca gttctgctcg cggcatcgac tatcacgatt cccgaaactc cggtttgctc
    32461 ggcgcacccg catacagtct ctcgattggg attgtactgc tggtgccact tacactcgtc
    32521 gctgctgctc tggtgtggaa aaagaaccgg gcaaggacga aataacgacg tctggttcga
    32581 gtgtctgcgt cggcagacgg aatgtgcgat tgtcagaccg tgattgcccg cagattgggc
    32641 atggcccgca atcaacaaca ctcaaggcaa tcagtatcga gcgagtcttt gtcagacgcg
    32701 cttagcccgg cgaggagtgg tcactttctt ccgctttcct ctctcctcat caccagacgt
    32761 aatggggctg acgttcgcca cggacgaaac gcgtggcatc ccaggcatcg atcgccaacg
    32821 agatggcatt cgtggcgatg agtgcgagga gccaggcctg gaacgcgacg tcctcttccg
    32881 ggaccgcggc aagacgcacg gccatccagg ctagcatagg tacccagatc agatgtgtca
    32941 ccccgagcag ccgaatgaag cgcgtgcgtt gatagataag cgcctgggcg agtagcgcca
    33001 ggcctacggc tgcgagggcg acctgagctt caatgtgcgt gatgaacagc agacaggccg
    33061 cgttgacggc aatgagccac gcgccccaca ttctttgaac gggccggaac cgaaccagaa
    33121 cgattccgag cagttcaaag acagaacgca taaagccgat tcgactcgtt gagtgggcca
    33181 gctcggatga tagtacagtg gtcctcatat cgtcctcctt ggtgcaggtg cgcgtgaccg
    33241 cacccggtgt tctgctcaac aacggttccc actctacggg acatttctct tggtgtctta
    33301 tccgggagtc tgattctctt agcaggggtc ggcgagggta cgttggctta tccaggcgtc
    33361 cggattcgcg tgccgaagcg ccgtgatgta agagtgaatc cactagtgga gtgaaacgtc
    33421 ttacggtagg tcacgagaaa tactcgatgt gcgtggggga ggataaccaa acaccgagct
    33481 aaagctctga agtcattgag ggggtccctg gttatctttt catgcggggc gaggaattgg
    33541 gctacagaga tccatttttg agccgataaa gaacattatt ctatgtcggc aaggatacgc
    33601 gatgaaagac aacaagacgg cggtgttgcg gaaactgacg gaagattctg ccatgcagct
    33661 cctggagggg gtgacgctgc cgcctcagcc ggggacgttg cttgatatta atcgcgagcg
    33721 ggccaaggca gagcctgatg tggcggccgt ggcgggcatc atcgagcggg atctgagtgt
    33781 ggcggcggcg gtgttgaaaa ccatcaacgc tccgtggttc gagttgccga aaaaggtcga
    33841 gtcggtgcgc caggcggtgg tgttgctggg gatgcccaat gtcgtcaacg tcgtaatcgc
    33901 cctggccatg aagagtgaac tacaggggaa caatcaagcg gggatagacc gcttcttcga
    33961 cgaagcgaac gacacggcga tggttctgtc gagcgtgatg cgtgaggtgc ccgtcatgtc
    34021 gacggacaaa gcctatctgc tcgggctctt tcgggattgc ggcatgccga ttctgctgca
    34081 acgccttccg cagtatgcgg aggtgctgca ggaggcggcg aaaaggccgg agcagtcgct
    34141 gccggacatc gaagatgcgc gcttaaaaac cgatcatgcg gtcgtggggg cgctcatgag
    34201 ccggcattgg catctcccgg aagaaatcgg cacaatcatt ctcaatcatc accggactcg
    34261 cgagctgttc ggcggaaagg cgcagaagct tgcctcgtcc gaagaaagta tccggaccgt
    34321 gatcgcggtt ctggcgatgg tggactgtat ttgccatcga ctccggacac gcaacgattc
    34381 accggagtgg gctgccatcg gcgaaaaggt cctggcgcat ctcaaactgg atcaagccga
    34441 attcgagcat ctcgcttggg atcagcagga tcggttcgcg gcctagcccg ggggtactgc
    34501 ctttctctga agatcggcga ggttcctgtc gaggcgcggg caatacgcgc tgcgtgcggg
    34561 ggagcgtcac cgagcaccat tccggaactc agaagaaatt cagaaggtcc ccgttttctc
    34621 ttcctgagct cttccagaac ccattcggtc gtcccgtggc cggagggtcg attccattcc
    34681 agactgcttc gccaacctcc gcacccaggc atcttcaccg ctcgggagcc ccgttgcgca
    34741 cgcgtttgga gtgattccag ctcctatgcg gtctgtgagc gattcacacg agccactcaa
    34801 tcgcgtggcc gcatcctggg ccggtcacta agccacgctg tctgcttggc gtcgccttga
    34861 tcccgccgcc acgggctcga ccatctatca agcaccactc cttttcagcg caaccaaggc
    34921 agcaaggcca gatccaaaaa gccaggccgc agccggaacg gggacggggc tcgggccgcc
    34981 ttccaccgtc aaattgattc gcgcgaacgt ggaggtgtta aacggaaggc tctcgacgtt
    35041 gacttgaagc gtattgccgg agaatgtgac gtcactgtcg gataagccaa gcgtggtcac
    35101 ggtacgatcg atcagtgcct gcctgatact gggcattgca gcattgtcga aagtgagctt
    35161 atatccattg cggaagcctg atgcgaatcg gaagtcaggg gcggaattag taaagttcat
    35221 atcgatgaac gtgtctccta tattgatcgc aatatcaact aagcggagcc cactcggatt
    35281 actgacctgc aaggctttga ggctggggaa ttcgacgcct ggctccttga ccgtcacggt
    35341 gttgaggctc gttagcttaa attctggact agtggaggtg gcctgaaaga ctgattggaa
    35401 cattacatct gttccgatca acgccgcctc gccggtctga gcgaccgcgc ataatgccaa
    35461 cagagccatc catgcagttt tcatagaaga cctttctctt agcgtgccga ttgtccaacc
    35521 tgagtggttt gatctcgact aagtagtaac cgaaatggaa tctatagtgg ttcgagggct
    35581 ctgtcaagtg agtaaagtgg tctcaggcac tgttgatcgt tgcagttcct ccatggcagg
    35641 ccagtcagga atcattgata ttgggccgca catagcatgg tgcataagcc gtgttccgtt
    35701 ccagctgccg atacatgatt cccggcacca tttctggtca gttttgagga catgagaccg
    35761 acggcagaac agggagagta ctactatact tttcagcgta gggtgctagg cttgaatggt
    35821 gtcattcagc aaggtgacgc aagtgaggaa gcccatgcct gatcgtttcc atccggatga
    35881 agcgctcaaa aacgaagcga tctctcaagc gatggaaggt gattttattg gcgcgcggca
    35941 gaccggagcc aagattgcgg cgcggcaaga ctttttggaa gcgtggatgt atatcctcca
    36001 cctgcagcgg gatcgcgggg acgtcatagg aattaaggag actatcctct cgtgccctaa
    36061 tccatcctgg ttgcaaggtc acagctatgc cgaattggcc cgacattttg caaaaatagg
    36121 aaacaagact gcggcgatcg agatcgcgaa gctcctcggg cgatccggag agatgatggt
    36181 ggtgtatcta tcggcgcccg tggatctgat cctcaaaggt gatttcgccg gtgccagaga
    36241 agccgtcgcc aaggttgaag atgaacaatt gagaaaccaa ctgaatgccc tggtggacag
    36301 agaggagcaa atccgcagtg ggcccactca gcaatctgtt ttgtaactga agaccacggc
    36361 gcaatcgacc cagagtcgat acgcgactac tcgaaagcct aaccccctcg gcgcatccct
    36421 tgcagccagt caaccggacg cttgctactg gtgccgctca cgattctcgc tgtccctctg
    36481 ctctggaaaa agcggggcaa gaaatgggca aggtgccgta tgcgagtctc tgtgccgggt
    36541 aggaaaatgt gtgatcacag gccttgtcct atctgttata ccggagccat ctcaggatga
    36601 cccattgctt cattggtggc ggtttattca atcgaagggg tccgttagcc ccgcagtatc
    36661 aactagtctc agtagaagtt gtgcgggttc agatcgttct caacgcacat ctcccacaac
    36721 ttacggtatc cgtcttcatg aacccaactc ttgacccgat caggcaccgg gccgagcaac
    36781 tgggccacgt gcaataacag ctggttcttc gtgaagacac gtcccccgat ccatagtggg
    36841 tactgcgact ccttggcgtg cagcagaaac tcgaagatgg ttgttgcggc cccctccccg
    36901 gattgaaggt tcacctcggc gagaccttct aagaacctat aggtgggatc ttcccagatt
    36961 ggtacgacgg ctctactggg cgcaccgctc gacagtcctt ctataagctt gaaaacgacc
    37021 tcagtcgcag acgaggtgtc tgtgatagcg acgatcccgc ctagcccgct gtgctttgca
    37081 tctacttgtt tcttggaggc gttgtgccag atgggcaaga gcggtttggt tccttcgatg
    37141 tgctggcgaa tgaggatgtc catttcgtag gaggtccacc ctttgtggag gtaagttgga
    37201 cttagcagga gcgttcctgt gcgagactca gccatgccta gttcgacaga gtcaagtaat
    37261 ctatcaccaa ctttgagcat gagcggagca aaccagaccg agataccgtt cgcacgaagt
    37321 ccgtatgcga tctgaaaggc aaaatcgttg tcctcggacg cataggaaag aaaggtatcg
    37381 tgcgtaggca taggattgtg ttctaggtca aacaatgagg gtaggcttgg ccatggttat
    37441 cgttgtaggt ggttctgtat ggcatatcgt ccgcttaggt cgcgcgatgc catcctccag
    37501 ttcgtcaata gcgatggtag agcgccagca tttacgacaa atacggttag ttctctggcc
    37561 tcatttgctt cttttaaatt ggagaaggtg ccttctggga atgttgttgc aatactcttc
    37621 cgaaatcgaa tgaaggggac tgtcgtaatg gtagctgccg acgcatcaag agttccatct
    37681 aggatgctga ctttgcttgg atcaaaactg caaatggcga tctctgcatt ggtgacgatt
    37741 acagggtaga ggaaggcgat taagcggtcg acctgaaatt gtgattggtg gaccaaaatc
    37801 tcttcgtccc ctaatccctc gacagagtca atgaccccag cagctagccg ttccaagatg
    37861 ggctggctct ttgagtcgtc actaggaaga acgcagattg aggactcgaa agattttgga
    37921 aagatgttaa cgttttccca tattcgcaca tgatcccatt cttgttggct tacgtttgcc
    37981 tcacctattc tcgcgttcat tggtgaggtt cccccaatct gtaagcatga agccaacggt
    38041 gtttcttgct tttcgccctc gggaatcaag aacaaccagt ggagctgtct tcgatcgtca
    38101 gcctttactc ttttgcactc aatgaccatc ctgtacgtca cgaagtgatc gtgcatcagg
    38161 acaaggtcaa tgaagccgga ggattcagtc tctttatttg tccagggatg ttcgcgcgtt
    38221 gcgattaacc aattgtgagt tgaatgagtc cttcgaacct catcctcgat ttttaactga
    38281 aatggaaagc cagacttcca gatgtgcttt aatgtctcgt cctgattggc catttagctt
    38341 tgccttctcc cgcctggaaa agcgtatgcc tcggtcatga ctataggcgc ctgggagctg
    38401 tcggtaattc ttgtctgata gccgcggtgg ccctcagcat tggtcaatat ctcaaaggtg
    38461 atcttatttc ctgagtggcc gatttcctta aactcgcctt tcatacagtt ctcccaaata
    38521 atcaactgag gattctactt tttcgttcta gctgggtcta ttgtttgttt attgattgga
    38581 ttacattcgc cctctgaggc ggcctggccg gttccctgcg tgcgcgcgtc caacgagggg
    38641 agtcttcgac cgcgcgttgc gcgagcatgg aggacggcca ggccagcctc tttccccctc
    38701 tcacctcggc caatacatga tcaccgtcac gccaaccaga cacaccatcg ccccgatgat
    38761 atcgaatcga tccggctcca cgtgatcgac cagccacccc cacagaatcg agaggatgat
    38821 gaaccagccg ccgtaggcgg catagacccg gccgaagtgc gacggctgat aggtcggcac
    38881 gatgccgtac agaatcaaga tgacggcgcc cagcaggccg acactccagt ggctaccatt
    38941 ccgccaccat tgccagacca agtatccgcc gccgatttcg aacagacccg ccagcacgaa
    39001 caaggcgatc gattgtgtga tgagcatggg ttctctcatt gtttttcttc tggtaagaaa
    39061 tcgacgtttc gttcgaattg acgacacgcg ctccaggtga cgtacccgag cccggccggt
    39121 tcgatggcga ggcgaggggg cggtatgaga tcatgacgac aacggacgtg caagtccaag
    39181 ggggcagcgc cttacgttca gaggaggctt ccatgaaact tctctccgtg caagtcggac
    39241 gtccccgcac tgtcagatgg catggcaaag ttgtctcgac cgggatctac aaagagccgg
    39301 ttgagggccg tctcccgctg cagctcttca atctcaaagg cgacgaacag gcggacctga
    39361 cggtacacgg cgggcgagaa aaagcggtgt acgtctatcc gtcggagcat tacccgttct
    39421 ggcggaaaga atatcccgac ctgccgtttc acccgggatg gttcggcgag aacttcaccg
    39481 tcgaggggat ggacgaacgg acggtgtgca tcggcgatca attccggatc ggcggcgccc
    39541 tggtcgtggt gacccagccg cgcatgcctt gtttcaaact cggcatccga ctcggcaagc
    39601 ccgatgcggt gaagcggttc catgcgagcg gtcgctgcgg cttctatctc ggcgtgctcc
    39661 aggaagggga ggttggtcca ggcgatctct gggaacaggt cgcccggggt gatgaacaga
    39721 tgaccgtcat cgaaagctac cggctgatga cccaatagcg gcaagcccag ttcacttttc
    39781 atctctcaac ttcccacaac aagcagggct tagtttgccc caaactgcgc gcgtccaacg
    39841 agggagagtc tgcgaccgcg cgttgtgcga gcagtgggga gatcatcagg ccgccatttc
    39901 tcttgcccca tcggcctaaa ccggggctgg gtgatcgtat tggaagttgg acggcatcct
    39961 tttctaatgc taggatacga acattctgat tgctgatgga cagcagagag acagaggttg
    40021 tctctgatcc tggaaaatgt aggtgatgta tgagcaaagt agaagcgatt gagcagcaga
    40081 ttgaaaaact ttcctccgat gaacttgccg cgtttcgccg atggtatgtg gcatttgatg
    40141 cagaggcatg ggatcgccag ttcgaaaatg atgtgaaggc cggaaggctt gatgccctcg
    40201 ctgaaaaagc tcttcgtgcc catacctcag gacagtctag gccgctttga ttcatcatac
    40261 ctcaccggaa ttttgggcct gttataatgc ccttcctgct acaatccgtg agcaagctga
    40321 ccgcgcgttt gaattattaa aggccaaccc tcgacatccg tcgctgcatt tcaagcaagt
    40381 aggccggttt tggtctgcac gtgcaggtct tcagtatcga gcggttggag tggaagcgcc
    40441 tgacggcatc gtctggttct ggatcggcac acacgcggag tacgacaaac ttatccagaa
    40501 gtaaggcagg cagccaaccg gtctttatcc cgccgcaagg ttcttttcag gttttccttc
    40561 ctacctcccc acaccaagag gggctcggtt tgccccaaac tgcgtgcgcg tccaacgagg
    40621 gccttccgag gccgcgcgtt gcgttttcca cgccccctca cagggaatgg ccggatcacc
    40681 cttcaactgc gcgcatggga cgagcacagt tttatcgtgc gcgtgttctt tgccacacca
    40741 ttctcatcag gggttggcct ggttggtctc cttactgcgc gcgtccaacg agggtctttc
    40801 gaggccgcgc gttgcgcgag cacaggagac catcaggcca gccccatccc tcccactctt
    40861 gcatctccgt cttttcccct cgtacaatga cgaactccaa gctaaccctt tggcattctg
    40921 attgtttcgg agtgccggac cgatacaagg gccgtcttca tgcgggttga gtatacaaag
    40981 ggcgagcggg cctcgcgcga gctgatcatg ctccagcgcc agtcgtccga agccgccgcc
    41041 ggccggaaga tgaaggtcat gctcatcttt ccaccggact ggtttccgtc cgaaccctat
    41101 ctgagtctcc cgtccctcac cgccgttctc cgccaagccg ggcaccaggt cgtccaaaaa
    41161 gacatcaatc tcgagatgtg ggattggtac ttcagcgagg actttctcaa gaaggtcttg
    41221 cgcaaggtgc cgcagcaact cgatcgcctc aggaagctgg ccaagaagcg cgagttggag
    41281 gattgggagc aggatctcca acttcagctc tgcgaagtga gccgccagcg aatcgatgaa
    41341 ctgatcaaga aagcggagca ggcgaaggcg atcatccgcg gggaaatatt ttacgaaatc
    41401 gatcagttag aatgggccat tcaagtcttt cgtgaagtca catcggtgat ctcgctggtc
    41461 tatgcgccgg cgcggatctg catgccgccc atggaaacca acctctccta taaggtgttc
    41521 gtctccagcg aagtcatcga ggcggtgaat gacacccagg tgaacgtgta ccgcgatgtg
    41581 ttcgagcaac tcgtgaagcc ggccatcgag gcggagcagc cggatgtgat cgggatctcg
    41641 atcgtgttgc agcagcagat gttctcctcc atgacgtttt gcgcgctcat caagcagcac
    41701 tttccccaca ttcatgtgac gatcggcggc aacacggtca cgcgtttgcg cgacgtgctc
    41761 ccggagtcgc cgctgttcca gtacttcgac agcgcggtgg tctatgaagg ggagacggcc
    41821 ttcgtgcagc tcgtgtcggc ggtcggggcg aagcagagtc tggccgaggt gccgaacacc
    41881 atctacaagg acgcgaccgg cgtgcatgtc tccacgacga gctttgcgga agacatgcac
    41941 agcttgccgc cgccggactt cgacggcctg ccgctggaaa aatatttcgt cccgaccaaa
    42001 atcctgccgt acctggcaac gcgaggctgt tattgggggc gctgcgagtt ttgcgatcac
    42061 ggtgaaggtt acacggcggg ctatcgctcg aagaaaattc aggacgtgct ggcggagatc
    42121 acctttttgc gcgacaagta cggggcccgg cattttcact tcaccgatga atcctatccc
    42181 ccggcgctgt tcagaaaact cgctcgcggc ttgatcgaca gcaatatggg aatcgtctgg
    42241 acgacccaca tgcggtttga gaagagcttg ctggaagacc aggtctggca ggatgcgaag
    42301 gagtctggct gtaaatatct ccactttggc tacgagtcgg gtaatgaacg ggtgctgaaa
    42361 ctgatggaca aggccaccac gaccgccatc atgaccgagc atctcaagcg cacggctgaa
    42421 gccggtatct ggaatcattg catggggttc ttcgggtttc ccggcgagac gcgcgaggag
    42481 gcctggtcgt ccgtgcagtt cctggagcaa aacaaagact acgtccattc gctgggcttc
    42541 ggcaccttcg acctcggccg gcataatccg gtggcgaaac atcccgagaa gttcggcgtg
    42601 accgcctata agaaccctga gtgggatctg gcgctggatt actacttcac cgtcaagcag
    42661 ggcctgagca ttgaagaggc ggagcgcgtg tttgaagagt tcgagcggaa ccacaacccg
    42721 ggctgggacc tgcggctgtt catccgcgaa tacattttcc tctacatcgc gcgcttcgga
    42781 ttgcagaagc tgccggatct gcaatatcgg tcggccaaaa tcatcggagc gtcgcccacg
    42841 cttgcgggga aaatgtaatg cgcaggaagc aggtgatcgg ccaatgagta gtaatggtct
    42901 cgtccaaatc gatggcctga ccccgatcaa gaaggaagat cggaagcagt cgaaggtgat
    42961 gttgctgttt ccgcccgagt gggtgccgac ggcgccgtat ctcgccttgc cttcgttgac
    43021 ggccgttctt cgcgaagccg ggcacacggt cgtgcagcgc gacatcaaca tcgagatgta
    43081 cgaccacttc ttcagcatgg agtttctgat ctgggtgaag ggtcgcttag gcatgcatct
    43141 caagccactc caggacaagg agaaggccgg cacgctgacc gaccaggaag ccagccagaa
    43201 agcggtgctg gaagacgcct atgcggtgga cgtgttcgat cttgcggagc gcgccgaaga
    43261 cgcgaagctg gttgtgcgcg gcgaacggtt ctacgaggcc gaaaaactgg agcgcgcgct
    43321 gaatacgttt cgtgaggcga tgcagtacat ctccgctgcc tattatccgg cctcgctcgt
    43381 gttttatccg atggaaagca acctcggcta tcgccccggc gtatcgaagg aagtgttcgc
    43441 ctgtctcgac gatgagcagg tgaatgtcta tcgcgacatc tgcaatcagc tggtgctgcc
    43501 cagcgtgagc aaggaaaagc cctcggtgat cggcatctcc atcggcacgc aaatgcagct
    43561 gatggccggt cttacgttct gcaagatgat caaagagagt tttccggaga tccgcgtcgt
    43621 ggtcggcggc aacgtcgtca cgcggttgca ggaagaatgg gcgcaccacg agcggttctt
    43681 taccgaggtg ttcgacgccg ccatcttgta tgaaggtgag catgcgctgc tctggtacct
    43741 ggaggccgtg aacggccagc gggcgataga ccaggtgccc aatctcatgt atcgcgacgc
    43801 ggacggcgtg aagcagagca aggaagtgta cacggagaaa acgtcggcgc tgccgttgcc
    43861 ggacttcgac ggcctgccgc tggataatta tttcgtgccc gagcggatca ttccctatct
    43921 tgccacccgc ggctgctact ggggccggtg caccttctgc gatcacggcc agggctattt
    43981 cgaccagtac cgcggcatga gcgcgcagca tgtggtcgag caggtcacgg cactgcggga
    44041 taagtacaac tgccgtcact tcctgttttc agacgagtcc tatccgcccg cgttgttcaa
    44101 gaaggtgtcg caattgctcg tggatcggaa cgtcggcatc aagtggacga cgttgatccg
    44161 cttcgaggag accttgcagg accaggcgat ttgggatctg gcggcgaagg ccggctgctg
    44221 cacgttgtac tacggcatgg agtcggcgaa cgagcgcgtc ctgaatctca tggacaaaca
    44281 tgcgcggaag agtgtgatcc agaacaatct gcatcaggcg gccaaggcgg ggatctggaa
    44341 ccacgtgatg gcgttctacg ggttccccgg cgagacgcgt gacgaggcgc tggagacgag
    44401 gcagttcgtc atcgacaatc agccggtgat tcattcggtc gagctgtttt attt
//
LOCUS       DAOQDS010000034        43787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_278_length_43787_cov_21.769048, whole genome shotgun sequence.
ACCESSION   DAOQDS010000034 DAOQDS010000000
VERSION     DAOQDS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 43787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43787
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_278_length_43787_cov_21.769048"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="PK589_10110"
     CDS             complement(<1..1619)
                     /locus_tag="PK589_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNJ19998.1"
                     /translation="MSQLLQRAHQAKLADEREWHLLLHYRANLFGGFTSEQDEPGFFL
                     SPDGKTDPEAELDSTLTQFFSPDLVGRSKQPAQCAFVARYAWLHERLAFDPGQLPVMR
                     CDRFERWFEEFHARSVTLVFPSAFMNNPASMFGHTLLRIDQEGQTPHTRILAYTINFA
                     ADVPKDEGLAYPVRGIFGGYRGYFSTIPYYLKVQEYRDMENRDIWEYRLNFDAPQIRR
                     LLMHAWELGNASFDYFFFKENCSYHLLSLLEYADPSLRLTDKFRFWTIPADTVRLLAD
                     QPGLVTDTAFRPSRVTLIRRKREQLTAAEHGLVQRLVRDAAVAQSDDVRSLPLSRQAF
                     VLDVASDYVRYTGERDESQATAARDHNRQLLTARSLLRIPSEELSILPFATQPELGHK
                     TSRASLGGGWRNNDSFEEVSVRTAYHDLLDPEPGYTPDAQIEIGSISLRHYNRADQAR
                     IERATLLNVVSLSPIDSLFRAPSWKLNVGMQTIRHGECQLCSNWSMNGGIGASTETHM
                     LRREVFFAFAEVEGNYSRAYEERHRVGGGASA"
     gene            complement(1759..2268)
                     /locus_tag="PK589_10115"
     CDS             complement(1759..2268)
                     /locus_tag="PK589_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HNJ19999.1"
                     /translation="MLRQIIVALGVVAVASQAGLALAANPDTGPGCGLGKLAWADYKN
                     QKNIAPQVMMATTNGTFGSGTFGISSGTSGCTNDGQVMADQKTTMFALLNFENLTQEM
                     AQGKGEHLASLATLMGVPAEQHAAFFALTQERYTALVQDGETSSVALVKTLNDAVSKS
                     PVLAQAVSR"
     gene            complement(2428..2874)
                     /locus_tag="PK589_10120"
     CDS             complement(2428..2874)
                     /locus_tag="PK589_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNJ20000.1"
                     /translation="MRIGVISDTHGLFDPSITRHFAGVEHILHAGDIGPRDVLAQLER
                     IAPVTAVSGNVDGFDASGIPVEQTIELAGHRIAVYHRLYEGGRLTGDGIDLLERIRPT
                     VCVYGHTHQPKAEWRDGVLLFNPGSAGPKRFRLPRAGSLAVSKWIN"
     gene            complement(2888..4021)
                     /locus_tag="PK589_10125"
     CDS             complement(2888..4021)
                     /locus_tag="PK589_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNJ20001.1"
                     /translation="MNATPNALCFGEGLPGAGVPCTVMLVEEGLRVSPMGESALAEQV
                     VTFADMAVEAGGLDHDQLVVSWGTGPSVWTLYVKDPALIVAFRRAAPPQLTAHLERAA
                     EQVRRARHSHRLLWGSVLGVVFGLGLLLWFGTDLMVEWAVARIPIEWEQKLGETVYQD
                     FLSKETVLKEGPAVSAVQEMTQRLTEKIPNNPYKFQISVVQSPVVNAFALPGGYVVVF
                     TGLMTKAESGEEVAGVLSHELNHVLQRHGMERMVKMLGLSAVVGILLGDQQGLTGLAK
                     QVGMELVTLKFGRAQETEADVTGIRLLSDARIAPDGMIRFFERLSEKDKERVEVFSTH
                     PMSAARAALLKAELAALPKRTPEPFTFQWKTIQDSLAVSEPKK"
     gene            complement(4196..4879)
                     /locus_tag="PK589_10130"
     CDS             complement(4196..4879)
                     /locus_tag="PK589_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNJ20002.1"
                     /translation="MKCEILYPGAFPMVRVYLDASETVKAESGAMVAASPTIDIESKM
                     EGGFLGALSRKMLSGEKFFFQTLRASRGAGEVLLAPTVPGEIVLLELDGVNEYMVQKD
                     GFLAGAEGVKIESKMQSLTRGLLGGEGFFILKIGGTGQLVLNSFGAIHKIELKPNEEY
                     IVDNSHLVAWSSTTTYNIEKASSGWIASLTSGEGLVCRFRGPGVVYIQSRNPGSFGNW
                     IRQFIPVSE"
     gene            5119..5454
                     /locus_tag="PK589_10135"
     CDS             5119..5454
                     /locus_tag="PK589_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20003.1"
                     /translation="MKRVVIEELTPGMILAKPVTNNTGLVVLPPGAELDEATLSRLQR
                     LGLTSVYVEGDAGDASGKTLAELEAELDHRFRLVSDDPIQCQIREAIRQQLRTTHGVA
                     DHQEAPPAT"
     gene            5451..6287
                     /locus_tag="PK589_10140"
     CDS             5451..6287
                     /locus_tag="PK589_10140"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNJ20004.1"
                     /translation="MKPSANVDLRRRIEQVGELPTLPHVVQKLASMIGRPNVSAEEIG
                     ALIEKDQVLSAKVLRLANSPFYGFPSRIASVAHAVVVLGLSVVKGLTLCATAFDMMKN
                     AGMNDLWRHSLGVAITAHILGAKAGMKNPEEVFVGGLLHDIGKVVLYVKWPDVGQQIT
                     AARKDGSRSLMETEQELFAVTHADVGGWLATAWHLPTTLREPILHHHMPTAAQDAKLQ
                     TAIVHVADVLVKGLACGNPGDDLIPPLSRQAWDLVGLDAQSLAQSLAQAAEEFQTIDD
                     YL"
     gene            6284..7249
                     /locus_tag="PK589_10145"
     CDS             6284..7249
                     /locus_tag="PK589_10145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNJ20005.1"
                     /translation="MSGSPSNRRILLLHHDPVEIERLTAGLSRAGFSVVVADAGRLAL
                     HELVHDPPCLVLAAEGTNGRSADTLAKELRADPFLGRLPVIILIRDSRVNDVDWAALG
                     VDDYIAVPYRPEEVPQRVRLCLSRLERSLDANPLTRLPGNSTILHETTARIESGAPFA
                     LAYLDLDNFKSFNDRYGYARGDEVLVVTCRILTTVVSELAGTDGFVGHVGGDDFVFMS
                     APATIEAICQTLIKRFDLVIPDFYDPDDRGKGFIDSVDRRGNHERFSLMSLSIAVVTN
                     EHRAITHPGDVSKIASELKKIAKSQPGSVYVKDHRKAETTDARTT"
     gene            complement(7280..7786)
                     /locus_tag="PK589_10150"
     CDS             complement(7280..7786)
                     /locus_tag="PK589_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HNJ20006.1"
                     /translation="MNVLLVGIMAVFIALSPAWAQGDQTGVPWSQLSPGEQQLLQRFS
                     DNWDQLPPRKQQRLRNGARQWGTMTPEERQEAKQRFKEWRSLPPEQRQQLRERYQQFR
                     QLPPERRESVRDARRWFRSLPPEQRKELREKFQTMSPEERRAYRRELRRQYGGSGGGQ
                     APAGVPGQ"
     gene            complement(8087..8443)
                     /locus_tag="PK589_10155"
     CDS             complement(8087..8443)
                     /locus_tag="PK589_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20007.1"
                     /translation="MNERSDEQPDPLSAAAKRLLDHRLQELDQKTVLALQRARVNAVS
                     RSSARRPWLQWAGGVALASMAALAVTMWLWQANGANHSHLLLEDLELMQSPENLELSE
                     DLEFYDWLADGTATTG"
     gene            complement(8440..8991)
                     /locus_tag="PK589_10160"
     CDS             complement(8440..8991)
                     /locus_tag="PK589_10160"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ20008.1"
                     /translation="MASLDQTQALDRFLAGIERRAFRMAHIATGNADEALDLVQDAML
                     KLAEKYARRPEEEWGALFHCILQSRIRDWYRRERVRNRLREFFRGAQDDEDAEDPLEQ
                     IPDSSTPAPDGEVQRKRACAELEVALRALPLRQQQAFLLRIWEELDVAQTARAMGCSE
                     GSVKTHLFRALQVLRQRLGEHWP"
     gene            complement(9404..>11752)
                     /locus_tag="PK589_10165"
     CDS             complement(9404..>11752)
                     /locus_tag="PK589_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20009.1"
                     /translation="PVKAEPITEFTLAGTPAESIETVLEAAPQVEDSLELVGAPEAMM
                     PSGSAPVDEIVEPIPLSSVEEQTVSLAEPIALEAQVEPPAEPSSEESEPAPVLALEPA
                     AKPDEHVVVDSVVTAAEVSLTELTEVPTASAEDAPLVAPHELSTVVPAEESSELVQIP
                     QALEAVQTDQPVDMAASPVPALEPEVPAPAVSAPAEVPASDGLHILWEDASSAPTPKP
                     STGNMLTRWLKKPAEPVAPEVSAVPPATPVPAEPVVPVASQAVEESVPVAVVMEQPYE
                     HIEQAPAAVVEEPTHRPTRIQSAGGGVWSRVAEAAASLVGAGVSTTRSLVVLVLALVG
                     LVLFVVGGTIAAVALTWLILEEQPNAAYRTMTSVPQQTLQDSSKNGYLLLLGLGAAAS
                     QDPVQVGLDRRIEGADRAFTHTCLTGEGYSPDERQSASADAAGKWMKAADPALQMQMD
                     VSGAKTWLSQAEVGLSRYRQWLNKPFEDVGYGQSISPNCGLILQTHRLYLADGFAQDV
                     DTGFARLETDLTAWRTVLGQAKSLPVKMLASAAMNDDAMIMDGLLQRPDLDERYVSRM
                     AKLAKPLEPSEQSLRWPMQSQFVLATKTLDEAVSQNRAETRPFYGAIAAALPLPKQRR
                     FNAYAEYYDAAGKAAADGRYTDLPKLSQFVRVAPYSVSDMVMNPIEGLVGVDSMPTWE
                     TYAGRVLETDARLRLVSLQAWLRRTPPEQDLLTRLAKAGQGLYDPFTGLPMLVNMKKG
                     VLYSVGPDLKDNEAHERLDLVAQIPSVAWAGGKKLADTGKAK"
     assembly_gap    11753..11852
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11853..13991)
                     /locus_tag="PK589_10170"
     CDS             complement(<11853..13991)
                     /locus_tag="PK589_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ20010.1"
                     /translation="MRSDKKPPLKSDKKVLPKVQKKGSLGYNKKSALLEDAITQMNAG
                     KYGRASSALKDLLALDPLNAEARRLFATLHLRLGSLMSARTAFESLAREAMERQDYWL
                     AESLLREYLTAGPRYVPFLEMLGRVYEEKGDVMAAVAEYGKAVEVLLEDPDTEKPNHP
                     SELFARIRSIAPGSPVSFRLAAMFDTVTGQVLQATPQPAVPTQEHEAPQADGVEAPAS
                     AVAPMPWEQIEPLQADVPVAQAPSSPSDVPPTAEVIEPALSAALPVEAVAAVESQKTE
                     IDQSPTLESSEVRTTLTSADSVAPAVPAVGSSDEPMKAAAESEPPAIDPLPALSGEVE
                     SAPPTIPVELPPTNVALVEVGADPIAHAPSSPAPMPWDQVEEVAVVIPPASPSQESTV
                     SDEVAPIALESEVGPAVTVEAVLPSMELPAGTGSQTTASTPLTPAPLSGNQVAEEIIA
                     IPPVTSEVSAVPESAQMPTSETEWSSAPPSDPAPRAIEVLSQSGSHPIVSTPTTPVSM
                     PWDQVEENPILIPPAAGSSAPSVEEVAPATCEETPLPPVIEAGKSDTEPPAQQAEAQT
                     AAPSASSSSEITSSGLSWDEILAAVAAMQSSPASPQSLPSAEPTPATESTVMEEPATA
                     VTAPSDAVEMTAVAPDAPSSEPSLDLGSASSTLSAPMPWEQVEVEDVAIPRQEPEPEF
                     GSATIAVEETGNSSGMALDSTLVVPAVPEPV"
     assembly_gap    14163..14262
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14263..15140
                     /locus_tag="PK589_10175"
     CDS             <14263..15140
                     /locus_tag="PK589_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HNJ20011.1"
                     /translation="LTGGETSVYCGRENLTDMQRILIFSDLDGSLLDPTTYSYKDASD
                     ALAAIEHRGATLILVSSKTRAEMEPLRRSLCNHHPFIVENGGAIYVPNGTFPFPLEGA
                     LSREAFQVFQLGAPYATLRAALKEIGQEIGGRLRGFGDLTLAEVVQLTGMSAGDAQLA
                     MQREYDEPFVMDGDVVAWQDLLAAATRRGLCCTRGGRFYHLMGANDKGMASRQLIEWY
                     RRLEQQKGQTLVTVGLGDSLNDLPMLESVDYPILIQKPNGSYDQDVQLPRLIRADGVG
                     PVGWNRSLIDLLPTL"
     gene            complement(15197..15418)
                     /locus_tag="PK589_10180"
     CDS             complement(15197..15418)
                     /locus_tag="PK589_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20012.1"
                     /translation="MTNKISDIDGEAKARRWLGGEAAVPRYPHRRPSRFVFNRSLLGL
                     VACAISAGFAGGLVVGLLSRRTNTSQRGH"
     gene            complement(15460..16167)
                     /locus_tag="PK589_10185"
     CDS             complement(15460..16167)
                     /locus_tag="PK589_10185"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20013.1"
                     /translation="MKEFFEKGLLDPLELMARQVLAVLPSLLAMSIIFFAGLVVAWTA
                     SQALERLLRVVGLDRLFDRLGVTGALLRGGVKTDPSRLVGQAAYWLVMIFATVAALGA
                     LNLQPINDFAKSFLAYVPHLVTAGLILVAGWLLSNFVSQAVLIAAVNAGLPPARLVAT
                     CSRWGIQLLAVAMALEQLGIAQNIVVVGFGITLGGIVMAGAIAFGLGAKDLAKDYLER
                     GLSVRTRDGAPDDLRHL"
     gene            complement(16225..17526)
                     /locus_tag="PK589_10190"
     CDS             complement(16225..17526)
                     /locus_tag="PK589_10190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ20014.1"
                     /translation="MTPGPQLPDATHTTPLTPETEAQVDQFAHTDILVGIPSFNNVET
                     IGHVVKAVSAGLAKYFPEARAVLVNSDGGSTDGTQQVVSQAVVDLKTLFIGDQQSSLH
                     KIITPYHGIPGKGSAFRTIFEIARRLKAKACAVVDSDLRSITPEWIELLVSPVFEQGF
                     DYVAPYYLRHKYDGTITNSIVYPLTRTLYGHRIRQPIGGDFGFSGQLAQHYLDKHVWE
                     SEVAKFGIDIWMTTEAIASGARVCQSFLGAKIHNPKDPAADLSAMLVQVLGAVFALME
                     DHYAVWGKTDGSNAVPLFGFQYEVGVEPVNVNVDRMISTFRQGLTDLGTIWDQILAPG
                     TRQSLRPLGTCSLQDFRIADSLWARVVYDAAVAYRNRVLPLDHVLKAFTPLYLGRTAS
                     FVLDTQGLTSSEAEGRIEALCQAFEKDKSYLVARWNEPHAG"
     gene            complement(17833..18201)
                     /locus_tag="PK589_10195"
     CDS             complement(17833..18201)
                     /locus_tag="PK589_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20015.1"
                     /translation="MMEQQNRPRFIVECTGSRSEDGLLVWNGRMLDLSIPGWSRTGLK
                     GLQAGDLLQLHLHIPGEPKPLSVRLATIRWASESRLGVDPILMDADDQLRLSDFVSAH
                     TTVPSFSTDRKEQIVISDAG"
     gene            complement(18389..18733)
                     /locus_tag="PK589_10200"
     CDS             complement(18389..18733)
                     /locus_tag="PK589_10200"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ20016.1"
                     /translation="MAKSPTTRSHSRVPVSCFLYYLGEGLVGTGKVCDLSVRGWRIEG
                     DKPVTVGMKLTLRVFLPDEPKAIDVEGVTVKWVKDRAFGLETLKLSAAAEARIHKFVE
                     SVLKSADSSRVA"
     gene            complement(18889..19188)
                     /locus_tag="PK589_10205"
     CDS             complement(18889..19188)
                     /locus_tag="PK589_10205"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ20017.1"
                     /translation="MRSFRRFPVQCSVYYSCDAFQGSGIAWNLSLNGWRVDGTHPVDP
                     GTVVTLCIFLPGQHPSVFVDRAIVRWSRGQEFGVEVNTIKADEQARLEQFVTALV"
     gene            complement(19296..19372)
                     /locus_tag="PK589_10210"
     tRNA            complement(19296..19372)
                     /locus_tag="PK589_10210"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19336..19338),aa:His,seq:gtg)
     gene            complement(19498..20214)
                     /gene="pyrF"
                     /locus_tag="PK589_10215"
     CDS             complement(19498..20214)
                     /gene="pyrF"
                     /locus_tag="PK589_10215"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNJ20018.1"
                     /translation="MMTRIDARDRLIVALDVPSAAEAEALVDRMGDQVRFVKVGLELY
                     TVAGPEIVRRLVDRGKRVFLDLKFLDIEETVRRATARVAAMGATFLTIHANRKALIAA
                     VQGRGQSDLKLLAVTVLTNFDGNDLRDMGIQRSVRDLVTARAMLAAETGCDGVVASGE
                     EPEAIRAKVGPNLLIVTPGVRPAGNATDDHARPTTPTQTIAAGADYLVIGRPIRDAQD
                     PPAATAAILKEMQAAFDHRG"
     gene            20326..20601
                     /gene="rpsT"
                     /locus_tag="PK589_10220"
     CDS             20326..20601
                     /gene="rpsT"
                     /locus_tag="PK589_10220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNJ20019.1"
                     /translation="MPVVHKSTIRRARQSEKRRLRNRATISSVRSILRKVQDAIAAKK
                     PDEAKATLRAATAALSKAVTKGVLKPNTASRRVSRLAVRVNAITASA"
     gene            complement(20585..21697)
                     /gene="holA"
                     /locus_tag="PK589_10225"
     CDS             complement(20585..21697)
                     /gene="holA"
                     /locus_tag="PK589_10225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNJ20020.1"
                     /translation="MSTSVMSMELPQSLARSGVMPLYAVVGEEDYLRDSSVAALRTAA
                     LGAGAESGFNYDIFHGDDSSIADVLACAAEIPVFAERRVVVYKSVEKLPAREGEKLLS
                     YLASPNETTTFIVVGAKLDGRLKWTQALTKRAVTVNCAPLRDAQLSAWIRQEAAALGV
                     RMQEDAIQLLKEVGGESLYAVKRELEKLAAYVPSDRTVQSSDVEALRGTEPGASVFDL
                     MNAIGAHDHGRAFRILARNVENGEAPLRILGALVWQYRRLWKLKEQLKAGGREGEAAR
                     TFRMDPSRVRGFLAQFPDDQMTQAFEWFLETDSKLKGGSGSTPVRVMEDLLFRLCGKP
                     SKPASAAQRVQPAVPPASRPSGSRPVSNVRTVTRKR"
     gene            complement(21717..22352)
                     /gene="lptE"
                     /locus_tag="PK589_10230"
     CDS             complement(21717..22352)
                     /gene="lptE"
                     /locus_tag="PK589_10230"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNJ20021.1"
                     /translation="MPRYLLLLVLGLCAVNITACGYQFRVQGAGPTIGGASEPAAKRS
                     QAPKLAILPISNSTYEPNFEIKLANYLRREFSAGAGAEIVGPSAGADLYLSGQIMQIL
                     LPTLSFDQTTTFESRVEMLVSVRIEDAKTKRLVWSQVAKGTAEFFLTQDLQFNRVLQN
                     RALEQAGRFVAEDLASRFLLFLDTGELDKMLKRPVKAEAVPGGNAPPEAAR"
     gene            complement(22357..24792)
                     /gene="leuS"
                     /locus_tag="PK589_10235"
     CDS             complement(22357..24792)
                     /gene="leuS"
                     /locus_tag="PK589_10235"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNJ20022.1"
                     /translation="MSKSYDHQAVESKWQAYWETHRPFRASDDPSKPKFYCLDMFPYP
                     SGSGLHVGHLEGYTATDIVSRYKRMRGFNVLHPMGWDAFGLPAEQYAVKTGVHPAITT
                     AQNIATFKRQMKRVGLSYDWERELSTTDQDYYRWTQWIFLKLFERGLAYVAEVPVNWC
                     PALSTVLANEEIVDGKSEVGGFDVIRKPMRQWVLKITAYAERLLEDLSLVEWPTSTLE
                     MQKNWIGRSIGAEVDFALADTTGNLRVFTTRPDTLFGATYMVVAPEHPLVDVVTTPSQ
                     RAQVADYRDAAARKSDLQRQELDKVKTGVFTGGYAINPVNHERLPIWIADYVLMSYGT
                     GAIMAVPAHDERDWAFATQYHLPIREVIQGGQVDQAAFVETERGRVINSTTPDGSCSL
                     DGLLPVDAIPKMTTWLETHGKGKKTINYKLRDWLFARQRYWGEPFPIVWVDGEPRPLP
                     EEQLPLTLPETKNFKPSGSGESPLANLEEWLTTTDPVSGQAARRETNTMPQWAGSCWY
                     YLRFIDPKNTRQLVDPAKEQYWMPVDLYIGGSEHAVLHLLYSRFWHKVLFDAGVVSTP
                     EPFKKLVHQGIVLGEDNQKMSKSRGNVVNPDEMIDQFGADAVRLYEMFMGPLESMKPW
                     STRGVEGITRFLDRVWRLMIGEDGALSAAVTNITPTAEQLRLLHYTIKKVTEDIESLR
                     FNTAISQMMVFTNDMTKLDQRPRALLEPFVLLLLPFAPHLSEELWERLGHQPSAGQQA
                     WPPFDPALVVSDRLTIPIQVNGKLRGKLEVDAGASRDQLEPLAKKEVAEWLQGKEPKK
                     VIYVEKKLINFVV"
     gene            25011..25640
                     /locus_tag="PK589_10240"
     CDS             25011..25640
                     /locus_tag="PK589_10240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ20023.1"
                     /translation="MSIYATYMFPRLMDWVLRGERFQTERRLVLAPAHGTVLEIGFGT
                     GLNLPHYPSTITDLHTVEPATLLPDRVARRVANAPFPVHLRQLSAEVLPYASGLFDCA
                     VSTFTLCTIPDPCAALREVRRVLKPGGCFLFLEHGRSDDPAIARWQDRLNPLQQVVAC
                     GCNLNRRIDRLITQAGFRVEQLDRFTLPGVPRIGGEMYRGRAGTAGRST"
     gene            25747..29688
                     /locus_tag="PK589_10245"
     CDS             25747..29688
                     /locus_tag="PK589_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ20024.1"
                     /translation="MAVSQMFPGNHPSSTSSSDPIDSVRETSLAFQRVALLYQAMPAA
                     LIAGLGCAVALVFVNWNVLPHDRLLAWLAYHLLLSIGRYGLARSFAATSPTPSTIRRW
                     DRAFLTGTLLAGLGWGATALLVFPESSLAHQVFLAFVLGGVSAGASSTLAARFDAFLS
                     FAVPTLAPLILRFFFLNHEQALPMAICTLLFSLLMVYTAYRTSNNVREMLQLKYHNAQ
                     LVDQLSSQLEEGEQTALLLNVHSEHYRFIMEYAQDIIYRTDRSGRFTFINPAVIRLLG
                     YHETEMLGHRALDLVHPNYRRSTEHFYIRQFLRKTPSTYYEFPLLTKTHQTLWVGQNV
                     QLLLRDQEVIGFQAVMRDISARKETEEALRFSQDRYRALFESSPEMLLTVDAEGRLLT
                     VNATAATELGYSADELVGQPISLIVHADDRSTIQQHFADCLRRPGHVLRGEFRKIRKG
                     GSPIWVREASRAVRNPNGQFDILIVCENVTERKGMEDALTHTRQLLESIVEHIPHMVF
                     LKDAKELRFAQFNKAGEQLIGLPREALLGKNDFDLFPREQAEFFTARDRDVLTGGALL
                     DIPAEPIQTKDHGLRWLHTKKLALYDHTGVPRYLLGISEDITDQKQRHEIEQRRLGQL
                     ATQQTVLHKLAEDPSIHSGHPQQAFPVMTEHAAGTFAVERASIWLFDGTQSTLVLQDL
                     FEATSNRHSQGTMLSTKEYPAYLHALETEPYSLAAHAAQSDPRTSELTPSYLIPLGIT
                     SMLDAPIRRHGRVVGVLCLEHTGTPKTWTSEEEAFAASLAAMATLTLEAADRRQAQEA
                     LEQHVRERTGELRRMTAHLQTIIEESPLAMIELDHTGHVTTWNAAATALFGWTREEVL
                     GKELPYVPAGQEQASNELWTSVMNGGAPRNLELCRQRKDGTLIDVNMWGTLLQGPGGQ
                     TTGSIGFFIDVTEHKQLEEQLRQAHKMEGIGRLAGGVAHDFNNLLTVINGCAMLLLEQ
                     VRAEDPLHRSLTEILTAGQRAAALTKQLLAFSRRQVLTFQVFDLNEALSSISSMIERL
                     IGEDIALVRDLTSQPCIIRADRGQIDQVVLNLAVNAKDAMPNGGTLTIATRTVTLTAD
                     QPVPHPAMLPGRYVRLSVRDSGEGMDRTTLSHIFEPFFTTKEEGKGTGLGLATVYGIV
                     KQSQGFIFADSNPGDGATFDMYFPLAQAPSPAAAPATPSRPHCGHETILLVEDQTAVR
                     MLLTQALSDYGYTLIEASSGQEALRLVAAAKTPIHILLTDVVMPQMTGPALAERLRQQ
                     WPSLRVLFMSGYAEGSVLPTFLAEPGTGFIQKPFLPTELAQKLRELLDSAK"
     gene            complement(29733..30419)
                     /locus_tag="PK589_10250"
     CDS             complement(29733..30419)
                     /locus_tag="PK589_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNJ20025.1"
                     /translation="MLQHRTDPTPHPVDWSHIDDVLLDMDGTLLDRHFDNFFFEEELP
                     RRYAAKHALAFAEARDRLMSMYRSVEGELAWTDLHYWTEQVQIDVVAMHRELDHMIGF
                     LPDAEAFLLSLRRLEKRVTILTNAHRAGVEVKTAKTGLHRQVDRIVDAFEVGWLKMRS
                     EYWPTCQELVGFDPSRALYIDDDEQCLAAAEQFGIRRIFHRSKSSSQASPVPSARFHS
                     IENFQSILPG"
     gene            30480..31874
                     /gene="der"
                     /locus_tag="PK589_10255"
     CDS             30480..31874
                     /gene="der"
                     /locus_tag="PK589_10255"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNJ20026.1"
                     /translation="MLPSQAMSRTKPARGPVTPLFTLEGGRLPIIALVGRPNVGKSTL
                     FNRILGTRAAIVDDVPGVTRDRNYADATYRNRHFRLVDTGGLDPTASEGMLSLIRQQS
                     QLAIAEADILVLVLDARAGLTPPDEEVVATLRGSDKPVFYVINKVDTPKADPLVADFY
                     RLGQEHLYPLSAEHGIGVAELLDDLFPHMVDPPGEETPTEIPRIAIVGRPNVGKSTLV
                     NSVLGEARVVVSNVPGTTRDPVDSIATFKDRQYVLTDTAGIRRRGRVERGIEGYSVAR
                     SLRAIGRSDVAVLLLDAEEGVTEQDTKIAGLVLKQGRACILVVNKWDLKTDDVDAREA
                     YKQDLERRFPFLAWAPVLFISALEQDFLRGLFALIDQVYFSFCKRVPTGALNQFFQGL
                     LEEHPLPVRKGKPAKASKSAFVTQVATRPPAFALFVGHPDNMTPAYLRFLENQIRKEY
                     HFSGTPIRLMVRKK"
     gene            32126..33172
                     /locus_tag="PK589_10260"
     CDS             32126..33172
                     /locus_tag="PK589_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20027.1"
                     /translation="MVTLVNSDQRFRCISLSLSALLSLSCLPAAGWAEPSAPLIEPPP
                     LEEPAASVEPVAPEALPVEPEPTQTVATAPVSPSVQVSGFIWRSKPGIVFLQTPIGPL
                     SLSSKTTLRDVKNSQKITLWVHGTTTVIDIRERGADKLVHRYVTAMPNFTSPEKQTVA
                     LWTPDGEQSFALGGLAAKIASRSDEQPFTIEVDGSGTVRGLHDVQYDLQVNQVPRTPS
                     SVHLLLNGTVSKLKSNYVFLKTPLGIVTVSGKTGVRNAKVGQEMSVWVQDRHVAIDLY
                     QDGVSTPSRRFLSGPLTYAPEGQSALIMQGPEGAQSIPLAQRPAALSAVKEGLPITVE
                     LGRQGELLDVRRVN"
     gene            33252..33884
                     /locus_tag="PK589_10265"
     CDS             33252..33884
                     /locus_tag="PK589_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ20028.1"
                     /translation="MASRKKSPPASSDGHGLPHSASHPFDQLYRDHVDVMYRFAYRLC
                     GEAEAAKDLVQETFLNAYRAYDRFRGDARVSTWLYAIASHACQRMRRKRKGEPERELS
                     LTEFVPTSEGEFALQIPMEGLSPQEALENKELREILDRAIAKLPRKYRMVLVLRDMEG
                     LSAKEVGDILGLSERAIKSRLHRARLFVRKELSGKGLAEEFHHAVTTVDR"
     gene            complement(34010..34135)
                     /locus_tag="PK589_10270"
     CDS             complement(34010..34135)
                     /locus_tag="PK589_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20029.1"
                     /translation="MLAQPDRLAKGIEEYFAIRAVAEVRADFLADLAGQLVVQIG"
     gene            34220..34684
                     /locus_tag="PK589_10275"
     CDS             34220..34684
                     /locus_tag="PK589_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ20030.1"
                     /translation="MTRPNPITAVPEPSATDSLSQTRSASRFLHACSSGLPIMVLLIG
                     TGILLAQPSDGLQRGQSLYREHCMDCHGTAGKGDGPKAPFLSPRPGNLVSAATSAKTD
                     KELLRTIAQGKPRTAMPGWKEALPEEDQQAVLQYIRSLIQFSRSPTATPPTP"
     gene            34772..36490
                     /locus_tag="PK589_10280"
     CDS             34772..36490
                     /locus_tag="PK589_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20031.1"
                     /translation="MIRGNLCHHRQTIAILALALAFGSWAGIAESAPAVGGKGHAKTG
                     TPKTTPAIQTAIRYAEALSQGDRVTAGQLDFACQYRHLTASPTKVKQFAPKDDPSYDA
                     CWQNLTDAYAPMLTRSDIAMDIIWPSTGPLLFFGDDLPRAPASAFVADAVGVAPPGSG
                     LHVTALNSRTIPSGSFKLARTGKVLAAPTTLVQLSVQYQDPLTSPVTYAAGNVKWTNT
                     IKRPRRALKSVTLQWVVFSGLKQHGFPGDTAVFNLPVPSQPEAPGMMTDKIPFTTETS
                     RALPESMAWWGPDDQPGVLTAAAARAAIFPDLRDRVALLNRVLIIDPKQPDALTVLTR
                     HLYSALLREARSSHQVPVKDPALGIVLDEFYWNIYAQGMRLDLSNGMEMGGLSQPTPA
                     DFLYRLIPALQTLAEIRPEQLDNRFRLGVAYRWNNDQIPMVETFEALVKDIPEHRRTP
                     KAEALLQLAWSRINKVSWNRILHDTETPRAYANAEASLAFAELPLDKFLAEYAMAYTM
                     IFLPNYGDKEKMLHHLTEAKRWFDEVPGKTNEAWRYFLHTGLLKAVLDADPIFAPILA
                     TVPSQP"
     gene            36761..37066
                     /locus_tag="PK589_10285"
     CDS             36761..37066
                     /locus_tag="PK589_10285"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNJ20032.1"
                     /translation="MNSDPPGANVLINGTSAGTTPLQYQVPRRGDLTVEVQKPGYTPQ
                     TRVTGRKLSSVGIVDVIGGALFLLPLLGLIAPGAWEQDPGIIGITLEPDHPQPAQPQ"
     gene            37116..38162
                     /locus_tag="PK589_10290"
     CDS             37116..38162
                     /locus_tag="PK589_10290"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNJ20033.1"
                     /translation="MIDVKGYAARTAKSRLAPFTFSRRNVGRQDVLIGIRYCGICHSD
                     VHQARDEWGGSIFPMVPGHEIVGVVEQVGASVKQFKVGQMVGVGCFVDSCRTCPQCKK
                     GEEQYCEGHISFTYNGKERDGVTPTYGGYSTKVVVDQRYVLRIPKQLRPEEAAPLLCA
                     GITTYSPLHHWGVGKGHRLAIVGLGGLGHMAVKIGKALGAHVTVLSHSDKKRRDAKRL
                     GAKDFYKTSDPKTFTTLAKQFDFILDTVSAPHDLDAQLELLKTDGTMILVGVPDKPAQ
                     LGAFPLILKRRRLVGSLIGGIQETQEMLDFCAKHKCGADVEVIPIQKVNEAYDRLVRG
                     DVRYRFVIDMGSLT"
     gene            complement(38346..39311)
                     /locus_tag="PK589_10295"
     CDS             complement(38346..39311)
                     /locus_tag="PK589_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNJ20034.1"
                     /translation="MTVRNIRFRQSVIRTVLGLAAMSGLAMQPVESAFAKVVEVSMTA
                     VETKLVIDGEGHTYDAWTFNGQFPGPVIRVQEGDEVVFSLTNPETNGRPHSMDFHAAE
                     TNFLEHYKEVRPGESLQYRFKAKWPGVFAYHCGASPMIQHIARGMMGAIIVDPKDPKA
                     MPKADREYVLVQSELFKDPDDVDGMFDRKYQHVVFNGAVFRYDPVHSKAGGAFLEAKP
                     GERVRFYFVNAGPNNVSAVHPIAEIWDDVWESGNPANHTRGVQTQLIPPAGAAILDMV
                     LDGNDGVYPIVTHSLTDALRGAIALLKVDKDAKQLPLMPLVEPAK"
     gene            complement(39471..40835)
                     /locus_tag="PK589_10300"
     CDS             complement(39471..40835)
                     /locus_tag="PK589_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNJ20035.1"
                     /translation="MSDVQRQIETYIKDIRPQYEDMLGQAVEIPSISMDPRHAPDVRR
                     MAELATQYLRAAGAEAQIVETPGYPVVSGGWTVDPHYPTVTVYNHMDVQPAQEPEWKQ
                     APFAFQNDHGIYRGRGATDDKGPALAALFGARYAIDQGVPINVRFLWELEEENGSPSF
                     ASAIKNRAAIPAPDSVVISDTIWLSKNQPAMPYGLRGLLGARLTLRTGTKDAHSGVTG
                     GAARNPLVELMEIAHACVDAKTGKVKIPGFYDEVVPPTKAEIASFLKSGFQVSKFKQA
                     YGFKTLRTHDPAEVMRRIWAAPTFEVHGLTGGYHGPGVKTIVPGHGELKISMRLVPGQ
                     TPEKIFALLKHYVAKLNPAVKVEREGMLHPFRGVFDGPYVEAVKRAVLAGFGKEPAFI
                     REGGSIGAVVTMQKAWKVPILFMGLSLPEHGYHAPNEYFDWGQASGGIKAFAHYFSDL
                     AAKR"
     gene            41035..41478
                     /locus_tag="PK589_10305"
     CDS             41035..41478
                     /locus_tag="PK589_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20036.1"
                     /translation="MGTALLACLALCLSGGNRVFAQEAVTVQEVLADPALFHLRQITI
                     KGTVRQVQPLDPYEIPAGSTCYGGYLFSLEDDTATLSIAVPGLCGVPMVKDPDVEDGA
                     RVVVEATVQAPSHGGYALSFKGAKIAMDQEGVVQAIANRITPLVE"
     gene            41482..42432
                     /locus_tag="PK589_10310"
     CDS             41482..42432
                     /locus_tag="PK589_10310"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ20037.1"
                     /translation="MTTRPLEQTARVAAPAHTRVGWIGTGVMGASMCRHLQAAHYRLT
                     LFTRNRGKASAILAKGATWADSPRAVAEQTDILITMVGFPRDVRQVYFGDHGVLAGAG
                     AGMVLVDMTTTEPSLAQEIAQAAQSRGVFAVDAPVSGGDIGARDATLSIMVGGDASAV
                     QTIMPLLECLGKKIVHQGHSGAGQHTKLCNQIVIAGTMIGVCESLLYGYKAGLELPRM
                     LESIRGGAAACWTLDNLTPRILARNFDPGFFVEHFIKDMGIALEEARRRQLTLPGLTL
                     AHQLYEKVEALGHGRSGTHALMLALEALSNIDPTRPSTSL"
     gene            42458..43177
                     /locus_tag="PK589_10315"
     CDS             42458..43177
                     /locus_tag="PK589_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20038.1"
                     /translation="MHPLVLALILTITCTACTEARGLNRHILQESFHDHPDVTTDRAI
                     AATMATQATLPTPYRLAIYFKHEDFPSRPALQRVDWVSSDGARVQRSLEPVQEEGILQ
                     RTFLLANSTVQGNTLRDIRLAAARYNADAILIIDGASAFERYNNGHAWWYATGIGAYF
                     AHGTECHALFMMEGTLWDVRTGYLYGTQTAEGETTLIGPAPSLDDKAAVAEAKDVAMA
                     RFGRELADTLRLLKEKQKAIP"
     gene            complement(43431..>43787)
                     /locus_tag="PK589_10320"
     CDS             complement(43431..>43787)
                     /locus_tag="PK589_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNJ20039.1"
                     /translation="NIADRPGHPRAFVTGGNMVARWHVAGPWTLVARPEFYWDRNGRW
                     TGSEQFVKAVTSTVEYKIPYKWTNTILRLEHRWDESTGAGGGFFRRGEVQPGVLSLTP
                     NQHLVLLGILWTFDSP"
BASE COUNT         9200 a        13688 c        12043 g         8656 t
ORIGIN      
        1 gccgatgcac cgccgcccac acggtgccgc tcctcgtagg ctctactgta attcccctcc
       61 acttccgcaa acgcaaaaaa cacttcccgg cgtaacatat gcgtctccgt ggaggccccg
      121 atcccgccat tcatactcca gttgctgcac aactggcatt cgccatgccg gatggtctgc
      181 atcccgacat tcaatttcca ggatggggca cgaaacaggc tgtcgatggg cgagagggat
      241 accacgttca gcagggttgc ccgctcaatc cgggcctggt cggcccggtt atagtgacgg
      301 aggctgattg agccgatctc gatttgagca tccggcgtat atcccggctc gggatcgagc
      361 aaatcgtgat acgcggtccg cacgctaacc tcctcaaagc tatcgttatt acgccagccg
      421 cccccgagac tcgcgcggga agtcttgtgc cctaattcgg gttgcgtggc aaacggcaga
      481 atcgagagct cctcggaggg aattcgcaag aggctgcgcg cggtcagaag ttgacggttg
      541 tgatcgcggg ctgctgtcgc ctgcgactca tcgcgttcac ccgtatagcg cacatagtcg
      601 gatgccacat ccagaacgaa cgcctggcgc gataacggca agctccggac gtcatcggac
      661 tgcgccactg ccgcatcacg cactaatcgc tggaccaatc catgctcagc ggcggtgagc
      721 tgttcgcgct tgcggcgaat gagcgtcacg cgcgaggggc gaaacgcggt gtccgttacc
      781 agtcccggtt gatcggcgag caggcgaacg gtgtctgcag gaatggtcca gaagcgaaac
      841 ttgtcggtca agcgtaacga agggtcggca tattccagta acgacaagag gtggtaggaa
      901 cagttttcct taaagaaaaa ataatcgaat gacgcattcc cgagctccca ggcatgcatg
      961 agtaatcgcc ggatttgggg cgcgtcgaag ttgagacggt attcccagat atcgcgattt
     1021 tccatgtccc ggtactcttg cactttcagg tagtagggta tcgtagaaaa gtatccgcga
     1081 tagcctccga aaatgccgcg taccggatac gccaatcctt cgtctttggg gacatccgcc
     1141 gcaaaattga tggtataggc caagatacgc gtatgcggcg tctgaccttc ctggtcgatg
     1201 cgcaacagcg tatggccaaa catggaggcc gggttattca taaaggccga gggaaatacc
     1261 agcgtgacgg atcgggcatg gaactcctcg aaccatcgct cgaaccgatc gcagcgcatg
     1321 accggaagtt gtcccgggtc aaaggcaaga cgctcgtgga gccacgcata ccgcgcgaca
     1381 aaggcacatt gcgccggctg tttggagcgg ccaaccaggt ccggcgagaa aaattgtgtc
     1441 agcgttgaat caagttcagc ctcgggatct gtcttgccgt caggagacag aaagaaaccc
     1501 ggctcgtcct gttcactggt aaacccaccg aagagattgg ctcgataatg gagcaagaga
     1561 tgccattcgc gctcatctgc cagcttggcc tgatgagcgc gttgaaggag ctgggacaag
     1621 tacggcgccg gcggctcttg cgcctcggtg gaagtcgcga ggaaaaggcc gactaggagg
     1681 atcaacaaaa tatgcacgat tggagacaga atgcagcgca gggccccggc caatgaccga
     1741 agccctgcga tgaatcaatt agcggctgac ggcttgggcg agaaccgggc tcttggagac
     1801 cgcatcattc agcgtcttca ccaaggccac ggacgaggtt tcaccatcct gcaccagtgc
     1861 cgtgtagcgc tcctgggtta aggcgaagaa ggccgcatgc tgttcggcag gcacgcccat
     1921 cagcgtcgcg agggacgcca agtgctcgcc ttttccttgc gccatttcct gcgtcaggtt
     1981 ttcgaagttc aacaaggcaa acatggtggt cttctgatcc gccatgacct gaccatcatt
     2041 cgtacaaccg gacgttccgg agctgatccc gaatgtcccg ctcccaaacg tgccgttcgt
     2101 cgtggccatc atcacctgcg gggcgatgtt cttctggttt ttataatcgg cccaggccaa
     2161 cttccccaga ccacagccgg gtcccgtatc aggattggct gccagagcca atccggcctg
     2221 tgatgccacc gccaccaccc ccaacgcaac gatgatttgt ctcagcataa gtcctcctcc
     2281 tcgtaacaag tgaatgactc cgcccgaccg actgtccata aatttctctt tcattataga
     2341 caacccccac caccatgtag agcaaaaaat ccgcgcatcg gcggttctcg tcattccgct
     2401 cgatcggcca acatgatgtg ccgcgtttca attgatccat tttgaaacag caagactccc
     2461 agcgcgcggg agacgaaagc gtttcggtcc ggcggatccc ggattgaaca acaagacccc
     2521 gtctcgccat tccgccttcg gctgatgggt atgcccatac acacacaccg ttggacggat
     2581 gcgctccagc agatctatcc cgtcccctgt gagtcgccca ccctcatata gtcggtggta
     2641 caccgcgata cgatggcccg ccagttcaat ggtctgctcc acaggaatgc cgctggcatc
     2701 gaatccatcc acattcccgg acacggcggt caccggcgcg atccgctcca gttgcgccag
     2761 gacatcacgg ggaccgatat ccccagcgtg cagaatatgc tccacccctg caaaatggcg
     2821 agtgatcgaa ggatcaaaca gcccatgcgt atctgagatg actccgatgc gcattccgct
     2881 ccttgcgtta cttcttaggc tcagacactg ccaatgaatc ctgaatggtt ttccattgaa
     2941 aggtgaacgg ctcaggggtt cgcttgggca atgcggccaa ctccgccttc aagagcgccg
     3001 cccgcgccgc gctcatggga tgcgtggaaa agacttcgac gcgttccttg tccttttcag
     3061 acagccgttc aaaaaatcgg atcatcccgt ccggtgcaat gcgcgcgtca gataagagcc
     3121 gaatgccggt gacgtccgct tccgtttcct gggcgcgccc gaacttcaac gtcactaact
     3181 ccatgcccac ctgcttggcc aggccggtca agccctgttg gtcacccagc agaatcccca
     3241 caacggcaga cagtcccagc atcttgacca tgcgttccat gccgtgacgc tgcaagacat
     3301 ggttcaattc atgactcaac acgccggcca cttcttcgcc gctttctgct ttggtcatca
     3361 acccggtgaa gaccacgaca tagccgcccg ggagcgcaaa ggcattcacc acggggctct
     3421 gcacgacgga gatctgaaat ttgtaaggat tattgggaat tttttccgtc aaccgctgcg
     3481 tcatttcctg cactgcactg accgcgggcc cttccttcag cacggtctcc ttggacagaa
     3541 aatcctgata cacggtttcg cccaattttt gttcccactc gatggggatt ctggccaccg
     3601 cccattcgac catcaggtcc gtcccgaacc acaacagcag ccccaggccg aatacgaccc
     3661 ccaacacact gccccacaag aggcgatggc tgtgtcgcgc ccgacgaacc tgctctgccg
     3721 cacgctccag atgggccgtc aactgaggcg gagccgcacg gcggaaggcg acgatcaagg
     3781 cgggatcttt tacatagagt gtccaaacgg atgggccggt gccccaggac accaccagct
     3841 gatcgtgatc caatccgccg gcttcaaccg ccatgtcagc aaaggtcacg acctgttcgg
     3901 cgagcgcaga ctcacccata ggactgacac gcaaaccctc ttcaaccagc atgacggtac
     3961 agggcacacc ggctcccggc agtccctcac cgaaacagag tgcgttggga gtcgcgttca
     4021 tagggcttgg actttcctca tgaagaacta tcgcctgtga agggggcgtt caaaacggcg
     4081 gccaacgcgg ccgcaggcga gaaaaaaccg gaggcgtacc ctatggggta cgttgaggat
     4141 tttttcgaac cgagaacaaa gttggcaggc gttttcagcg cccgccattc agccgctact
     4201 ccgaaacggg aatgaattgt ctgatccagt tcccgaaact gccgggatta cggctctgga
     4261 tatagacgac acccggcccg cggaaacggc acaccaaacc ttcgcccgag gtcaagctgg
     4321 caatccagcc tgacgaggcc ttttcgatgt tgtaggtcgt cgttgagctc caggcgacca
     4381 ggtggctgtt atcgacaatg tattcttcgt tcggtttcaa ttcgatttta tgaatggccc
     4441 cgaagctgtt gagaaccaac tgcccggtcc cgccgatctt cagaataaaa aacccctccc
     4501 cgcccagcaa tccacgcgtc agactttgca tcttgctttc aatcttcacg ccttcggcgc
     4561 ccgcgagaaa cccgtccttc tgcaccatgt actcattgac gccgtccaac tccagcagca
     4621 cgatttcgcc gggcaccgtc ggcgccagca agacttcccc cgcaccccgg ctggcccgca
     4681 acgtctggaa gaaaaatttc tcaccactca acatcttccg cgaaagggcc ccgagaaacc
     4741 cgccttccat cttgctctcg atatcgatgg tcggcgaagc ggccaccatg gcgcccgact
     4801 ccgccttcac cgtctcactg gcgtccagat acacccgcac cattggaaag gcccccggat
     4861 aaagaatttc acacttcatc gcccgctccc ttcatgaact gtcagcctca tcatgcgatc
     4921 gattctgtgg aatgtccccg gtcctgtcaa gcagactgtt gagacggacc gtccctccgg
     4981 ccacacggca cctgttttcc gctcaagatc gatcacgttt cagccgatac agcccaaaga
     5041 gctatcctca ggtgctgacg ggcaaccgct cttgtcggcc ttcccgctta tggactcgaa
     5101 gagacacgag agccaggcat gaaacgcgtc gtgatcgaag agctcacacc gggaatgatt
     5161 ctcgccaagc ccgtgaccaa caatacgggc ctagtcgtcc ttcctcccgg cgccgaactg
     5221 gatgaagcaa ccctctctcg actccagcgc ctgggcctca cgtccgtcta tgtcgagggc
     5281 gacgccggcg atgccagcgg aaagaccctt gccgagttgg aagccgagct cgaccaccgc
     5341 ttccgccttg tgagcgatga cccgattcaa tgccagattc gtgaagcgat ccgccagcaa
     5401 ctccggacga cacatggggt agccgaccac caggaggcac ctccagccac atgaagcctt
     5461 cggccaacgt agatctccgg cgacgcatcg agcaggtcgg cgaattgccg accctgcccc
     5521 atgtcgtcca gaaactggcg tcgatgatcg gccgccccaa tgtctcagcc gaggaaatcg
     5581 gcgccctcat cgaaaaagac caggtgctgt ccgccaaggt attgcggttg gccaattcgc
     5641 cgttctacgg atttccctca cgcatcgcct cagtcgccca tgcagtcgtc gtcctggggt
     5701 tgagtgtcgt caagggcctc acgctgtgcg ccaccgcgtt cgacatgatg aaaaacgccg
     5761 ggatgaatga cctttggcgc cattcgctcg gggtggccat tacggcacat attctaggcg
     5821 caaaagccgg catgaagaat cccgaagagg tgtttgtcgg cggactgctc cacgatatcg
     5881 gtaaggtggt gctctacgtc aaatggccgg atgtcggcca gcagatcacg gccgccagga
     5941 aagacggctc acgatcgctc atggaaacgg aacaggaact atttgccgtg acccatgccg
     6001 acgtcggagg gtggctcgcc accgcctggc atctgccgac cactctgcgc gagccgattc
     6061 ttcatcacca tatgccgacg gcagcccaag acgcgaagct gcagaccgcc atcgtgcatg
     6121 tggcggatgt gctggtgaag ggcctcgcct gcggcaaccc cggcgacgac ctgatccctc
     6181 cactttcccg tcaggcctgg gatctcgtcg gcctcgatgc gcaaagtctc gcgcagagcc
     6241 tcgcccaagc cgcggaagaa tttcaaacca tcgacgacta tctatgagcg ggtcgccttc
     6301 caaccgacgt atcctgctgc tgcaccatga tcccgtcgaa atcgagcggc tgacggctgg
     6361 cctcagccgt gcaggattca gcgtcgtcgt ggccgatgcc ggacggctag cgctgcatga
     6421 attggtgcat gatcccccct gcctggtgtt ggcggcggaa gggacgaatg gacggtccgc
     6481 ggataccctc gcgaaggagt tgcgcgcgga tccctttctc ggccggctgc cggtgatcat
     6541 tctgattcgg gatagccggg tgaatgatgt ggattgggcg gcgctcggcg tcgacgacta
     6601 tattgccgta ccctatcggc ccgaggaagt accacaacgg gtgcgcctct gcctcagccg
     6661 cctggaacgg tcgctggatg ccaacccgct tacgcggttg cccggcaaca gcacgatcct
     6721 ccacgaaacc acggcgcgga ttgaaagcgg cgctccgttc gccctcgcct acctggatct
     6781 cgacaacttc aaatcgttca acgaccggta tgggtatgcg cgaggcgatg aagtcctcgt
     6841 ggtgacctgc cgcatcctca ccaccgtcgt cagtgaactg gcgggcacgg acggattcgt
     6901 cggtcatgtg ggtggggatg attttgtctt tatgagcgcg ccggccacga tcgaagccat
     6961 ttgtcagaca ttgatcaaac ggttcgattt ggtgattccg gacttctacg atcccgatga
     7021 tcgcggcaag gggtttatcg actccgtcga ccggcgcggc aaccacgaac ggttttctct
     7081 catgagcctg tccatcgccg tggttaccaa cgaacatcga gccattaccc atccgggcga
     7141 tgtcagcaag attgcctccg agctcaaaaa aatcgccaag agccagcccg gcagcgtcta
     7201 tgtgaaggat catcgaaaag ccgagacgac tgacgcccgt accacctgac gcatcacggc
     7261 ctacgaactc gccggaagat tactgaccgg ggactccagc aggcgcctgc ccgccgccac
     7321 tgccgccata ctggcggcga agttcccgcc gataggcacg acgttcctcc ggggacattg
     7381 tctgaaactt ttcccgcaac tccttccgtt gctccggagg aagagaccgg aaccatcgcc
     7441 tggcgtcccg caccgattcg cgccgttcag gcggcaattg gcggaactgt tgataccgct
     7501 cacgcaattg ctgccgttgc tccgggggaa gcgatcgcca ttccttgaat cgctgcttgg
     7561 cttcctgacg ctcttcgggc gtcatcgtgc cccactgccg cgccccattc cgcaaccgtt
     7621 gctgtttgcg cggggggagc tgatcccagt tgtcgctgaa ccgctgcaac aattgctgct
     7681 cgcctggact gagctggctc catgggaccc cggtctggtc accttgcgcc caggcaggcg
     7741 acaatgcgat gaacactgcc atgattccta ccagcaacac attcatgggc cggtttgcat
     7801 ccttatgggg ctgcgagggc gatccttcgc agacggtgcc ggcttctctg gcgcaggctt
     7861 ctgagcgtct gaagacattc cgctgcgatt cggttgtggt tccacggaag actgcggcac
     7921 ttgatcgctg gtgatggtaa agggatcgac ccactgtcca tcctcatcct gccagctacc
     7981 caggaaatca aggaactcgg ggtccggctc ctctgccggc cgtgccggta ccgatgccgc
     8041 ctctgctatt ccggtggcca agaagaacag gagcatcagt cctgcactac cccgtcgtgg
     8101 cagtaccatc ggccagccaa tcgtaaaatt cgaggtcctc ggacaattcc agattttccg
     8161 gcgattgcat cagttcgaga tcttccaaca gcagatgcga atggttggcg ccattcgcct
     8221 gccacagcca catcgtcacc gccagagcgg ccatcgaggc cagcgccacc cccccggccc
     8281 attgaagcca gggccgccgc gcagaggacc ggcttacggc attcacccgc gcacgctgca
     8341 gggccagcac cgtcttttga tcgagctcct gcaggcggtg atcgagaaga cgcttcgccg
     8401 cggctgataa aggatccggc tgctcgtccg atcgttcgtt catggccaat gttctcccaa
     8461 tcgctgccgc agtacctgca gcgcccggaa caaatgggtc tttacgctgc cctcggaaca
     8521 ccccatcgca cgagccgttt gcgcgacatc cagttcctcc cagatgcgca gaagaaacgc
     8581 ctgttgctga cgaagcggta aggcacgcaa cgccacttcc agctccgcac aggcccgctt
     8641 acgctgcacc tcgccatccg gtgccggagt gcttgaatcg ggaatctgct ccagcggatc
     8701 ctccgcgtcc tcatcatcct gagccccacg aaaaaattcg cgcaggcggt ttcgaacccg
     8761 ttcccgccga taccaatcgc ggatgcgact ttggagaatg cagtgaaaca gcgcgcccca
     8821 ttcttcttca ggccgccggg catacttctc cgcaagcttc aacatggcat cctggacaag
     8881 atccagcgcc tcgtccgcat tccccgtggc aatatgcgcc atgcggaatg cgcgccgctc
     8941 aatcccggcc agaaaccgat ccaatgcctg ggtttgatcc agagatgcca ccctctcacc
     9001 caggcacagg tggcctggct gtccaggaac ggcgatgggg gaatggcagc cgctcatccc
     9061 catgctcgtc ttctatctgc ccagtcatcg accgatgagg ccccgtaggc agcagccgca
     9121 tggacccggt atcggtcgag atcggcccga ttttcgactt gaaacacggt gaaatcaaga
     9181 gaatccaagg aaatctccgc tcgcgacgca acgacgagtc ctgaaacgga tgcgccggac
     9241 tcgcagccac cctgcctgct tcacagcgag agtgcggctc tttcaggcta taacgcgaga
     9301 agacaatccc ggttgacagg atgggggcag gaccgcggca cggtgatggg agcaacactg
     9361 cgacgtattc ggtgagaggc ggggagaaga attgcggacg gaactacttg gctttgccgg
     9421 tatcggccaa ttttttgccg ccggcccagg ccaccgaggg gatctgagcc acgagatcaa
     9481 ggcgctcgtg cgcttcattg tccttgaggt ccggtccgac actatacagg acgccttttt
     9541 tcatattgac cagcatgggc agcccggtaa aaggatcgta gagcccctgg ccggccttgg
     9601 ccagccgggt cagcaagtcc tgctccggcg gggtccggcg taaccaggcc tgcaacgaga
     9661 ctaaccgcaa ccgcgcatcg gtttccagaa cccggccggc ataggtttcc catgtgggca
     9721 tggagtccac cccgaccagg ccttcaatcg gattcatcac catgtcgctc acgctgtacg
     9781 gagccacacg cacaaactgg gagagcttcg gcaggtcggt ataccgtccg tcggctgccg
     9841 cttttccagc cgcatcataa tattcggcgt aggcattgaa ccggcgttgc ttcggcagcg
     9901 gcaacgccgc agcgatggcg ccatagaacg ggcgcgtctc ggcccggttc tggctgaccg
     9961 cctcatccaa cgtcttggta gcgagcacaa attggctctg catcggccaa cggagggact
    10021 gttcggacgg ctctaacggc ttcgccaatt tcgccatgcg gctcacatat cgttcatcga
    10081 gatccggccg ctgcaagagc ccgtccatga tcatcgcgtc gtcattcatt gccgcactgg
    10141 ccagcatttt caccggcaac gacttcgcct gtcccaagac cgtccgccag gccgtgagat
    10201 cggtttccag ccgcgcgaac cccgtgtcca catcctgcgc aaatccgtcg gcaagataca
    10261 gtcgatgcgt ctggaggatg agcccgcagt tcggactgat cgactggccg tatcccacat
    10321 cttcaaacgg cttgttcaac cattggcggt agcggctgag gccgacttcc gcttgcgaca
    10381 gccacgtttt ggcgccgctc acatccattt gcatctgcaa cgccggatcg gcagctttca
    10441 tccacttgcc cgccgcgtcg gccgaggcac tctgcctttc gtcgggcgaa tacccttctc
    10501 cggtcaagca ggtatgtgtg aaggcacggt cggcgccttc aatccgtcga tccaggccaa
    10561 cctgcacagg atcctgactc gcggccgctc cgaggccgag caagagcaga tagccatttt
    10621 tactggagtc ttgcagtgtc tgttggggca ccgacgtcat ggtgcgatag gctgcgttcg
    10681 gttgctcctc aagtatcagc catgtcaggg ccaccgcagc gatggtaccc ccaaccacaa
    10741 acagcacgag gccgaccaaa gccagaacca ggacaacgag cgatcgcgtt gtcgacaccc
    10801 ctgctccgac cagagacgcc gccgcttccg ccacccggct ccatactccg cccccggccg
    10861 attggatgcg cgtgggacga tgggtcggct cttccacaac tgccgcagga gcttgctcga
    10921 tgtgttcata cggctgctcc atcacgactg caacaggaac ggattcctcc actgcctgtg
    10981 aggcgaccgg cacgacaggt tcagccggta cgggagtcgc cgggggaacg gcgctgactt
    11041 caggggcaac aggctccgca ggctttttga gccagcgagt cagcatattt ccagtggaag
    11101 gtttcggtgt cggtgctgac gaggcatctt cccacaaaat atgtaatcca tcgcttgcgg
    11161 ggacctcagc tggagcggag acagccggtg caggcacctc tggctccaaa gcagggaccg
    11221 gggaggcagc catgtctaca ggctggtctg tctgcacggc ctcaagagcc tgggggatct
    11281 gaacaagctc gctcgactct tctgccggta caacggtcga cagctcgtgc ggggccacca
    11341 gcggagcgtc ttcagctgaa gcagttggca cctccgtcaa ctctgtgagc gacacctcgg
    11401 cggcagtcac cacagaatca acaaccacat gctcgtccgg ctttgcggcc ggctccagcg
    11461 caagaaccgg tgcaggctca ctctcctcgg acgaaggctc cgccggcggt tcaacctgtg
    11521 cctcgagagc aatcggttca gccagggaaa ccgtctgctc ttcaacagaa gacaagggaa
    11581 taggctcgac gatctcgtct accggtgcag agcctgaagg catcatcgcc tccggcgcac
    11641 cgactaactc cagcgagtcc tccacttgag gtgcagcctc taagaccgtc tcaatcgatt
    11701 cagcaggcgt tccggcgaga gtgaattccg tgatcggctc cgctttgaca ggnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncactggct ccggcaccgc aggcacaacg
    11881 agggtggaat ccaatgccat tccactagag ttcccggttt cttcgactgc gatggtggcc
    11941 gaaccaaatt ccggttccgg ctcttggcgg ggaatggcga catcttcgac ttcgacctgt
    12001 tcccagggca tgggcgccga caacgtcgat gaggcgctgc cgagatccaa agagggttcg
    12061 gacgagggcg cgtctggagc gaccgccgtc atctccacag catccgaggg cgccgtgact
    12121 gcagtagccg gctcttccat cacagtggac tctgttgccg gcgtcggttc cgcgctggga
    12181 agcgactggg gcgacgcagg agatgattgc atcgcggcaa ccgctgcaag aatttcgtcc
    12241 caggacagtc cggatgacgt gatttccgag gaactggagg cggagggggc tgccgtctgc
    12301 gcctctgcct gctgtgcggg aggctctgta tcggactttc ctgcctcgat cactggcggc
    12361 aacggcgtct cttcacatgt tgccggcgca acctcctcca cactgggcgc ggaagaccct
    12421 gccgccggag gaatgagaat ggggttctct tcgacctgat cccagggcat cgacaccgga
    12481 gtcgtcggtg tggagacgat cgggtgactg cctgattgcg acagcacttc gattgcgcgt
    12541 ggagcaggat cactaggagg agccgatgac cattcagttt cagaggtcgg catctgcgca
    12601 gactctggca cagccgatac ctcactcgta accggcggta tcgcgatgat ctcctctgca
    12661 acctgattcc cagacagagg agcaggcgtc aagggcgtag aagccgttgt ctggctgccg
    12721 gtccctgccg gcagttccat ggagggaagc actgcctcaa cggtgacggc gggtccgact
    12781 tcagattcaa gggcaatcgg agccacttcg tctgaaaccg tcgattcctg actcggagat
    12841 gcgggaggaa tgacgacggc gacctcttca acctggtccc acggcatcgg tgccgggctc
    12901 gaaggcgcat gcgcaatcgg atcggcgccg acctcgacca acgccacgtt cgtcggcgga
    12961 agctccacag gaatcgtggg aggcgctgat tccacctcgc cgctcaacgc ggggagcgga
    13021 tcgatagcag gcggttcgct ttcagctgcg gccttcatcg gctcgtcgga ggagccaact
    13081 gcgggcacag caggagcaac agaatccgcc gacgtcagag tcgtacgcac ctctgatgat
    13141 tcgagcgtgg ggctttgatc aatctcggtc ttttgtgact caaccgctgc cacagcctcg
    13201 actggcaatg ccgcagacag cgccggctcg atgacttccg ctgtcggcgg cacgtccgat
    13261 ggactggacg gggcctgagc gacaggcaca tcagcctgca ggggctcgat ctgttcccag
    13321 ggcatgggcg cgacagcact ggccggagct tctaccccgt cagcctgagg tgcttcgtgt
    13381 tcctgggtag gaaccgcagg ctgaggggta gcctgcaata cttgccccgt aacagtatcg
    13441 aacatggcgg caaggcggaa cgagaccgga ctcccgggag cgatcgagcg aatccgtgcg
    13501 aaaagctcgc tggggtggtt cggcttctcg gtgtcgggat cctccagcaa gacttcaacc
    13561 gctttgccat actcggccac tgccgccatg acgtcgcctt tttcctcgta gacgcgtccc
    13621 agcatttcca ggaagggcac gtaccgcggc cctgccgtca ggtattcccg caacagcgac
    13681 tcggccagcc aataatcctg ccgctccatg gcctcccggg ccagggattc gaacgccgtc
    13741 ctggcgctca tgaggcttcc aagccgcaga tggagggtgg cgaagaggcg cctggcttca
    13801 gcgttgaggg ggtcgagcgc cagcaggtct ttgagggcgg aggacgcacg gccatacttg
    13861 ccggcgttca tttgcgtaat ggcgtcctcg agcagcgcgg actttttgtt gtagccgagg
    13921 ctgcccttct tctggacctt ggggagaact tttttatcgg acttcagggg aggtttttta
    13981 tcggatcgca ctgtataacc ctataacttt acacgcatcc gagaatggta tggaccggct
    14041 acgggtctga cgagtccatg gggaacctga cagagccatg tgccttacct tttcggcatt
    14101 ctttcgtgcg atcttgatta gggattttcc tgtgccggtt ttgacacaga gacgagagag
    14161 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtttgacc ggcggggaga
    14281 cttccgtata ctgcggcagg gagaatttga ccgatatgca gcgcatcctc atcttttctg
    14341 acctggacgg gagtctgctg gaccccacaa cctactccta caaagacgct tcggatgccc
    14401 tggcagccat cgaacatcgc ggggcgacgc tcatcctggt ctccagcaag acccgtgcgg
    14461 aaatggagcc acttcgccgc tctttgtgca atcaccatcc cttcatcgtc gaaaacggcg
    14521 gggcaatcta tgtccccaac ggcacgtttc ccttccccct tgagggagca ctctcgcgcg
    14581 aagctttcca agttttccaa ctcggagctc cctatgcgac acttcgagcg gcgctaaagg
    14641 agattggtca agaaatcggt ggtcgattgc ggggatttgg ggatctgacc ctggcggagg
    14701 tggttcagtt aaccggaatg tcggctggag acgcacagtt ggccatgcag cgggagtatg
    14761 acgagccctt cgtgatggat ggcgacgtcg tagcgtggca ggatctactt gcagcggcga
    14821 ccaggcgcgg tctctgctgc acgcgaggag gccggttcta ccacttgatg ggcgcgaacg
    14881 ataagggaat ggcgagccga caactgattg aatggtatag gaggctcgag caacagaagg
    14941 ggcagactct cgtgacggtc ggcctgggag acagcctcaa cgatctaccc atgctggaaa
    15001 gcgtggatta tcctattctt atccaaaaac ctaacggctc ctacgaccag gacgtgcagc
    15061 tcccccgcct catccgggcc gatggagtcg gacccgttgg atggaatcgc agcctgattg
    15121 atctcctccc gactctctga cgatcgagtc tacgacatcc cgctccagcc aacccacatg
    15181 agacatgagt gacggatcaa tgcccgcgct gagacgtgtt ggtccggcgg ctcaacaggc
    15241 cgaccaccag ccctcctgca aatcccgcgc tgatggcgca ggccacaagc ccgagaagcg
    15301 aacgattgaa cacaaaccgg ctcgggcggc ggtggggata ccgcggcacc gcagcttccc
    15361 cacccagcca gcgtcgcgcc ttggcctcgc cgtcgatatc cgaaatctta ttcgtcatac
    15421 catgtccttt atcagtgaac agccaagaac gctcagccgt tagagatgcc gtaagtcatc
    15481 cggtgcgcca tctcgagttc gaaccgacag cccgcgctcg agatagtctt tggccaagtc
    15541 tttcgcgcct aatccgaagg caatcgcccc ggccatgacg atccctccca gagtaattcc
    15601 aaacccgacg accacgatat tttgcgcaat accgagctgc tccagcgcca tggccacggc
    15661 tagcaattga atcccccacc gggagcaggt cgccactaat cgagcaggag gcaatcccgc
    15721 attgaccgcg gcgatcagca ccgcctgcga cacaaagttg gacaacaacc agcccgctac
    15781 caaaatgagc cctgccgtga ccaaatgcgg cacataggcc aaaaacgact tggcgaaatc
    15841 gttgatcggt tggagattca gtgcgcccag cgcggccacg gtggcaaaaa tcataaccaa
    15901 ccaataggcc gcttgcccca ccaaccgaga cggatccgtc ttgaccccgc ctcgcaacaa
    15961 agcaccggtc acgcccagtc gatcaaacaa ccgatccaac ccgaccacgc ggagcagccg
    16021 ctcgagcgcc tggctcgccg tccacgcgac cactaaaccc gcaaagaaaa tgatggacat
    16081 ggccagcagg ctcggcaaca ccgcgagcac ctgccgcgcc atcagctcca gaggatcaag
    16141 gagccccttt tcgaagaatt ccttcatact tccctcctcc ggcctcgact gcagcctgcc
    16201 ttcagatgga tggattcatg actctcaacc ggcatgcggt tcgttccagc gcgccaccaa
    16261 gtaggactta tccttttcga acgcctggca cagcgcttca atgcggccct cggcctcgct
    16321 cgacgtgagc ccctgagtat ccaacacaaa cgaggcggtc cggcccaaat ataagggtgt
    16381 gaaggctttc aacacgtgat cgagaggcag gacacgattc cgataggcga cagctgcatc
    16441 gtagaccacc cgcgcccata gactgtcggc aatccgaaaa tcctgtaagg aacaggtgcc
    16501 aagtggccgc aggctctgcc tggttcccgg agcgagaatc tggtcccaaa tcgtacccaa
    16561 atcagtgagg ccttgccgaa aggtgctgat catgcgatcg acgttgacgt tgaccggctc
    16621 aacgccgact tcgtactgga acccgaacaa cggcaccgcg ttcgaaccat ctgttttccc
    16681 ccacactgca taatgatctt ccatcagcgc gaacactgca ccgagcacct gcacgagcat
    16741 agccgaaaga tcagccgccg ggtctttggg gttgtggatt ttggcaccga gaaaactctg
    16801 gcagaccctc gccccgctgg cgatcgcttc cgtcgtcatc cagatgtcga tgccgaactt
    16861 tgcgacttcc gactcccaga cgtgcttgtc caggtaatgc tgcgccaatt ggcctgaaaa
    16921 tccgaaatcc ccgccaatcg gttgcctgat ccgatgccca tagagggtgc gcgtcaatgg
    16981 atagacgatg ctgttcgtga tggtgccgtc atatttgtga cggagatagt agggcgcgac
    17041 gtagtcgaac ccctgctcaa agaccgggct caccaggagc tcgatccatt ccggcgtgat
    17101 gcttcgcaaa tccgaatcca ccacggcaca ggctttggct ttcaaacgac gggcaatctc
    17161 aaaaatcgtg cgaaatgcgc tgccttttcc ggggataccg tgatagggcg taatgatctt
    17221 atgaagcgaa ctctgctggt cgccgataaa cagggttttg aggtcgacaa ccgcctgtga
    17281 gaccacctgc tgcgtgccat ccgttgagcc gccatccgaa ttcaccagga cggcacgggc
    17341 ctcggggaaa tatttggcca ggccggcgct gaccgctttg acgacatggc cgatcgtttc
    17401 cacattgttg aagctgggaa taccgaccaa aatatccgta tgggcgaatt ggtcgacttg
    17461 cgcctcggtt tcaggcgtga gcggggttgt atgggtcgcg tcaggaagct gagggccagg
    17521 agtcatacgt caatctcgca cgcctccgcc ctagcaacac tgttatgaca tgaccgaaat
    17581 ggaccggcga aaggctggtg tgtgtgacaa tgtgtcttgg ctaagagaca ccatcttgcc
    17641 caacgatgtc aagcaattcg gcttcccacc tgacatcagg gcatctctac cagagcctat
    17701 cagagcctga tcgatgcaga gcgacgccgc cttcgatgat cggagcgctc attttcaaaa
    17761 tctggcttca gcggaatttc gacacgatcg aggagtgagg gggaaatagt gttcagggag
    17821 tgagggaact gcttacccgg catcagaaat gacgatttgt tcctttcgat ccgtactgaa
    17881 cgacggcacc gtggtgtggg cactgacgaa atcgctcaat cgcagctgat cgtcggcatc
    17941 catgagtatg ggatcgactc cgagccgcga ctcactcgcc caccgtatcg tagccaaccg
    18001 taccgacagg ggcttgggct ctcccggaat atgcagatgc aattgaagga ggtccccggc
    18061 ttgcagtccc ttgagtccgg ttcgcgacca tcccggaatg gacaggtcaa gcatccggcc
    18121 gttccagacc agcaacccgt cctcggaacg tgagccggta cattcgacga taaaccgcgg
    18181 acgattctgt tgttccatca ttcctccagc caaacgcctc ggcagaatat gaaaaacaac
    18241 agattgtggg atagtccccg aaagtggggg atcaacggaa acagagggtg cggtggagtg
    18301 cggcgatgag cgacaaggcg ggcacgctac ggaatctctt tttcaagctg gacagtgagg
    18361 ccggacgaca agcaacgact cgattaacct atgccacgcg ggaggaatct gctgatttca
    18421 acacgctttc gacaaacttg tgaatacgcg cttcagccgc ggcactcaac ttcagggttt
    18481 caagcccgaa tgcacggtcc ttcacccact tcacggtgac gccttccaca tcaatcgcct
    18541 tcggctcatc gggcaaaaag acgcgcaacg tcaatttcat gccaaccgtg acgggtttat
    18601 ccccttcgat ccgccaaccc ctgacggaga gatcgcacac tttgccggtt ccaaccaggc
    18661 cctccccaag ataatagagg aaacagctga caggcacgcg agaatgggat cgggtcgttg
    18721 gtgatttggc catagtgtga gcaaagggat gagcactgcg cgcatcaacc ttgactaggg
    18781 accagtctac gcaattcgct gacaaggacc aagcagattt tcgttcccga caaggcggac
    18841 acctattgat cacgcactga tcgagaccat gaaggaggat aggataggtt agactaacgc
    18901 cgtcacgaac tgttcgaggc gggcctgctc atcagccttg atcgtgttca cttcaacgcc
    18961 gaattcctgg ccgcgggacc aacggacaat cgcgcggtcg acaaacacgg atggatgctg
    19021 accgggaaga aagatacaga gggtgacgac cgttccaggg tctaccggat gtgtcccatc
    19081 gacgcgccac ccattcaggg aaaggttcca ggcgatacca cttccctgga aggcatcaca
    19141 ggaataatac accgagcatt ggacgggaaa gcgacgaaag gatcgcacgt ggaatcgtac
    19201 accagccaca gaaagacgat gtgggactga cggtacgagc tgcttattgc cagcaggcat
    19261 ggtatcccca ctcgaaccct gctcctcgac tacgttgggg tgagtgacgg gtttcgaacc
    19321 cgcgacctcc ggatccacaa tccggcgctc taaccagctg agctacaccc accgtacaag
    19381 gtcagcagag aacgcgcatc ttaacaaacc ctgcagaaca gtcgcaaccg ccctcatcgc
    19441 ctcaatcgca ggagcacatc ttttcggacc tgctcctcaa aagtcacctg atctccgcta
    19501 tccccgatga tcaaacgccg cttgcatttc cttcaggatg gctgctgtcg ccgcgggagg
    19561 atcttgcgcg tcacgaatgg gacgcccgat caccagatag tctgccccgg cggcaatggt
    19621 ttgagtcggt gtcgtgggcc gcgcgtgatc atcagtagca ttgccggccg gcctcacacc
    19681 aggggtgaca atgagtaaat tcggtcccac cttggcacgg atcgcttccg gctcttctcc
    19741 tgaagccacg actccatcgc acccggtttc agccgccaac atggcccgag ccgtgactaa
    19801 atcacgaaca ctccgctgaa tacccatatc ccgcaggtcg ttcccgtcaa aattggtgag
    19861 caccgtgacc gcgagcaact tgagatcgga ctgcccacgc ccctgcactg ctgcgatcaa
    19921 cgccttccgg ttcgcgtgaa tcgtcaaaaa tgtcgcgccc atggccgcaa ctcgagccgt
    19981 tgcgcgccga acggtctcct caatatcgag gaacttgaga tcgagaaaca cgcgcttgcc
    20041 gcggtctacc aatctccgca caatttcagg acccgccacc gtgtaaagtt ccaggcccac
    20101 cttcacaaac cgcacctgat cgcccatacg atcaaccaag gcctctgcct ctgccgcaga
    20161 aggaacatcc agcgcgacaa ttaaccgatc tcgtgcatca attcgcgtca tcatcactcc
    20221 accctggtga ccaggaggcc ccgaagccgc tgatcctttc agcatccttg acctatcttc
    20281 gtcacctatg ttacaagctc aagctttgaa cgtaggagaa tccgtatgcc tgttgtccac
    20341 aaatccacga ttcgacgtgc tcgccaatcc gaaaaacgcc gcttgcgcaa ccgcgcgacc
    20401 atcagctcgg ttcgcagcat cctccgcaag gttcaggatg cgatcgcggc gaagaaaccg
    20461 gatgaagcta aggccacctt acgtgccgcc acggcagctc tgagcaaagc ggtcacgaaa
    20521 ggcgtgttga aaccgaacac ggcctcacgc cgcgtgtccc ggctcgctgt gcgcgtgaac
    20581 gccatcaccg cttccgcgta accgttctga cattcgacac cggtctcgat cccgatgggc
    20641 gcgaggccgg tggtaccgcc ggttgcaccc gctgcgctgc tgatgccggt ttcgacggct
    20701 tcccgcacag tcgaaacaag agatcttcca tcacacggac cggggtactt ccgcttccgc
    20761 ccttcagctt cgaatccgtc tccaagaacc actcaaacgc ttgagtcatc tggtcatccg
    20821 ggaattgcgc taaaaatccc cgcactcgcg aaggatccat ccgaaacgtc ctcgcagcct
    20881 cgccttcccg cccaccggct ttgagttgct ccttcagctt ccaaagacgc cggtattgcc
    20941 agactaaggc gccaaggata cggagggggg cctccccgtt ctcgacattc ctcgcaagaa
    21001 ttctgaaggc tcgcccatga tcgtgtgcgc cgatagcatt catgagatcg aagaccgatg
    21061 cgccgggctc ggtccctctg agcgcctcca catcggacga ttgtacggtc cggtctgacg
    21121 ggacataggc ggcaagcttc tccaactctc gcttcactgc atagagggac tcacctccga
    21181 cctccttgag caactggatc gcatcctcct gcatgcgcac acccaacgcg gccgcttcct
    21241 gtcgaatcca ggccgagagc tgagcatccc gcaggggagc gcaattgacc gtcaccgccc
    21301 gcttcgttaa ggcctgcgtc catttgagcc gaccgtccaa cttggccccc accacaatga
    21361 acgttgtagt ctcattcggc gaagccagat acgacagcag cttttcgccc tcccgagccg
    21421 gcagcttctc cacggactta tagaccacga cgcggcgctc cgcgaatacc ggaatctccg
    21481 ccgcgcatgc taagacatcg gcgatggagg agtcgtcgcc gtgaaaaata tcgtaattga
    21541 atccactctc agcgccggcg cccagggctg ccgtgcgaag cgcggcgacg gaggagtcgc
    21601 gcagataatc ctcctcaccg acgacggcat agagcggcat taccccgcta cgtgcgaggg
    21661 actggggcag ttccatgctc atgaccgacg tactcaccta cagacaccct ccaacatcaa
    21721 cgggcagcct caggtggtgc atttccaccg ggaaccgcct cggctttgac gggacgcttc
    21781 aacattttgt ccaactctcc agtatccagg aacagcaaaa agcgagacgc aagatcttct
    21841 gccacgaatc gacccgcctg ttccaacgcc cgattctgca gcacgcggtt gaattgcaga
    21901 tcttgcgtga gaaaaaactc cgcggtcccc ttcgcaacct gcgaccacac cagtcgcttg
    21961 gttttggcat cctcgatccg cacactcacc agcatttcga cgcggctttc aaaggtggtg
    22021 gtctgatcga aactcaaggt cggcagtaaa atctgcatga tctggccgga cagatacaag
    22081 tctgccccgg cggaggggcc gacaatttcc gcccccgccc ctgcagaaaa ctcccggcgt
    22141 aaataattgg ccagcttaat ctcgaagttc ggctcgtagg tgctattgga gatgggaaga
    22201 atcgccagtt tcggtgcctg agaccgcttt gctgctggtt ccgacgcgcc accgatcgta
    22261 ggaccggcgc cctgtacccg gaactggtag ccgcaggcgg tgatgttcac cgcgcacaga
    22321 cccaagacaa gcaacaacag gtagcgaggc atggcatcac accacgaaat tgatcaactt
    22381 cttctcgacg taaatcacct ttttcggctc tttcccctgc agccattcgg cgacttcttt
    22441 tttggcgagt ggctccagct ggtcgcgtga tgccccggca tccacctcga gctttccacg
    22501 cagcttgccg ttgacctgaa ttggaatcgt gagtcgatcg ctgaccacca aggccggatc
    22561 gaacggaggc caggcttgtt gcccggcact cggttgatga ccgagccgtt cccagagttc
    22621 ctcgctgaga tgcggcgcaa agggcaacag caacaatacg aacggttcca acaatgcgcg
    22681 gggccgctga tccaatttcg tcatgtcgtt cgtaaacacc atcatctgtg aaatggcagt
    22741 attgaatcgc aaggactcaa tatcttcggt gactttttta atcgtgtagt gaagcagtcg
    22801 cagctgctca gcggtcggtg tgatattcgt caccgcagcg ctcaacgcgc cgtcttcgcc
    22861 gatcatgagc cgccagaccc ggtcaagaaa gcgggtgatg ccttccaccc cgcgtgtgct
    22921 ccagggcttc atcgactcga ggggtcccat gaacatttcg tacaagcgga cggcatcggc
    22981 tccgaactgg tcgatcatct catcaggatt caccacattg ccgcgcgact tcgacatctt
    23041 ctgattgtct tcgccgagta caatgccttg atgcaccaac tttttgaacg gctccggcgt
    23101 gctcaccaca ccggcatcga acaacacttt gtgccagaac cgactgtaca gcaaatgcag
    23161 cacggcatgt tcactgccgc cgatatagag gtccaccggc atccaatatt gctctttcgc
    23221 cggatcgacc agttgccggg tgtttttcgg atcgatgaac cgcaggtaat accagcagga
    23281 acccgcccat tgcggcatcg tattcgtctc gcgccgcgcc gcttggccgg agacaggatc
    23341 cgtcgtcgtc aaccactcct cgagattggc caacgggctt tcgccgctcc cggacggctt
    23401 gaaattcttt gtctcaggca aggtcagcgg caactgctcc tccggcaatg gccggggctc
    23461 gccgtcaacc cagacgatag gaaacggttc tccccaataa cgctggcggg cgaagagcca
    23521 atcccgaagt ttgtaattga tagttttctt gcccttgccg tgggtttcga gccaggtcgt
    23581 cattttggga atggcgtcca caggcaataa cccgtcgagc gaacaggacc cgtccggcgt
    23641 ggtcgagttg atcacacggc ctcgctccgt ctcaacgaat gccgcctggt ccacctggcc
    23701 gccctgaatc acctcgcgaa tcggcagatg atattgagtg gcaaaggccc agtcgcgttc
    23761 atcatgagcc ggcaccgcca tgatcgcgcc ggtgccataa ctcatcagca catagtcggc
    23821 gatccagatc ggcaggcgtt catgattgac gggattgatg gcatagccac ccgtgaagac
    23881 gccggttttg actttatcca attcctgacg ttgcagatcg cttttgcggg cggccgcgtc
    23941 gcgatagtca gccacctgtg cccgctgcga cggagtcgtc accacatcga caaggggatg
    24001 ttcaggcgcc accaccatgt aggtcgcacc gaacagcgta tcaggccggg tcgtgaagac
    24061 acgcagattt cctgtcgtgt cggccagggc aaaatcaact tcggcgccga tcgagcggcc
    24121 gatccagttc ttctgcatct cgagcgtgct ggtcggccat tcgaccaggc tcaaatcctc
    24181 gagcagccgc tccgcatagg cggtgatctt caacacccat tgccgcatcg gcttgcggat
    24241 gacatcgaat ccgccgacct cgcttttgcc atcgacgatt tcttcgttcg ccaacaccgt
    24301 cgacaacgcc ggacaccaat tgaccggaac ctccgccaca taggccaggc cacgctcgaa
    24361 tagtttcagg aagatccact gcgtccaacg atagtagtcc tgatcggtcg tgctgagttc
    24421 gcgttcccaa tcgtaggaga gccctacgcg tttcatctgc cgcttaaacg tcgcaatatt
    24481 ttgagcggtg gtgatggcgg gatggacacc ggttttcacg gcatattgtt cggccggcaa
    24541 gccgaacgca tcccacccca tgggatgcaa cacgttgaag ccgcgcatcc gtttatagcg
    24601 ggacacaata tcggtggcgg tatacccttc caggtgcccg acgtgcagcc cggaccctga
    24661 tggataggga aacatgtcca gacaatagaa cttaggcttc gaagggtcat ccgaggcccg
    24721 aaacgggcgg tgggtctccc agtaggcctg ccacttcgat tcaacagctt ggtgatcgta
    24781 ggatttgctc atcgtatgaa catcataggt gacgtggtga ttggcgctcg actgaagaac
    24841 ggacgactgt agcatacgcg gtacgcggca acaaggacgc agcggagaaa gggactctga
    24901 atgggcacgt ccgcttcgct tgatcctcag cgccactcct gctcaaatac acggcgagac
    24961 ggcagcaaga gcggcaccaa ccgcgcccat tcgcccgtcg aggaatgccc ttgagtattt
    25021 acgccaccta catgttcccc cggctcatgg attgggtgct ccgcggagag cgcttccaga
    25081 ccgaacgccg cctcgtcctt gctccagccc atggaacagt gctggagatt ggctttggta
    25141 cagggctgaa cctgccgcac tacccgtcga ccatcacgga cttgcatacg gtggagcctg
    25201 cgacgctttt gcctgaccgc gtcgcccggc gggtggcgaa cgcgcccttt cccgtccacc
    25261 ttcgccagct gagcgcagaa gtgttgccct atgcctcggg tctgttcgat tgcgccgtca
    25321 gcaccttcac cttatgtacg attccagatc cctgcgccgc cttgcgagaa gtccggcgag
    25381 tcctgaaacc tggcggctgc ttcctgttcc ttgaacacgg ccgaagcgat gaccctgcga
    25441 tcgcccgctg gcaggaccga ctcaacccgc tgcaacaggt ggtggcctgc ggctgcaatc
    25501 tcaatcgacg catcgatcgc ctcatcacgc aagccggttt tcgcgtggaa cagctcgatc
    25561 gttttacctt gcccggcgtc cctcgcatcg gcggagagat gtatcgaggc agggctggca
    25621 ccgcgggtcg ctccacgtag taattcatat gttacaggga aattgtatcc ctcccgccaa
    25681 ttttgtagac tgcgcctata caagagtgtt ttgcatgtat tcttctcgta aacgggagtg
    25741 atcccgatgg ccgtctccca gatgtttccg ggcaaccatc cctcctcaac ctcatcgtcc
    25801 gacccgatcg attcagtcag agagaccagc ctggcatttc agcgcgtcgc cctgctctat
    25861 caggccatgc ccgcggcgct cattgccggc ctcggatgcg ccgtcgcgtt ggtcttcgtc
    25921 aattggaacg tcctgccgca tgatcggttg ctcgcctggc tggcgtatca tctgctctta
    25981 tcgatcggtc gatatggact ggctcgttcg ttcgccgcta catcgccgac gccctcgacg
    26041 atacgacggt gggaccgggc cttcctgacg ggaacgttgc tggccggact cggttggggc
    26101 gctacggccc ttctcgtctt tcccgaatcg tcactggctc atcaggtctt cctggccttt
    26161 gtgttgggag gcgtgtctgc gggcgcgtcc tcgacgctgg cggcgcgatt cgatgcgttt
    26221 ctgtcctttg ccgttccgac cctggccccg ctgattctgc ggttcttttt cctcaaccat
    26281 gagcaagccc tgcccatggc catctgcacg ctgctgtttt ccctgctgat ggtttatacg
    26341 gcataccgaa cctccaacaa cgttcgggag atgctccagc tcaaatatca caacgcacaa
    26401 ttagtggatc agctctcgtc gcaattggaa gagggagagc agactgcgct gctcttgaat
    26461 gtgcacagcg agcactaccg cttcatcatg gaatatgcgc aggacatcat ttatcgaacc
    26521 gaccggagcg gccggtttac gttcatcaat cccgcggtta tccgcctgct gggctatcac
    26581 gagaccgaaa tgctcggtca tcgcgcgctg gatctggtgc acccgaacta ccgccgcagc
    26641 acagaacact tttatatccg acagtttctt cgaaaaactc ccagcaccta ttacgaattc
    26701 ccgctgctca cgaaaacgca tcagaccctg tgggtggggc aaaacgtcca actgctgctt
    26761 cgggaccagg aggtcattgg attccaagcc gtgatgcgcg acatctcggc tcgtaaagaa
    26821 accgaggaag ccttgcgctt cagccaggac cgataccggg cgctgtttga gagcagcccc
    26881 gaaatgttgc tgaccgtcga tgcagaaggc cgattactca cggtgaatgc gacggccgct
    26941 acggaattgg gatatagcgc cgacgaattg gtcggccagc cgatttcatt gatcgtgcac
    27001 gccgacgatc gttccaccat ccagcaacat tttgcagact gccttcgacg accgggacat
    27061 gtcctccgcg gggaattcag aaaaatccgc aaaggcggga gcccgatatg ggtacgagaa
    27121 gcctccagag ccgtgcgcaa cccgaacggg cagttcgaca tcctgatcgt ctgcgaaaac
    27181 gtcaccgaac ggaaggggat ggaggacgcg ctgacccaca cgcgccagct cttggagtcc
    27241 atcgtggaac acattccgca catggtcttc ctgaaagatg ccaaggagct ccgcttcgcc
    27301 cagttcaata aagccggcga acaactcatc ggcctcccac gggaagccct cctggggaaa
    27361 aatgatttcg atctcttccc ccgtgaacag gcggagttct ttaccgcaag ggatcgggat
    27421 gtgctcacgg gcggagccct tttggacatt ccagccgaac cgatccagac caaggatcac
    27481 ggcctgcggt ggctgcatac caagaaactc gccctctacg accacaccgg cgtgcctcgg
    27541 tatctcctcg ggatttccga agacatcacg gatcagaaac agcggcatga aatcgagcaa
    27601 cgccggttgg gacaactcgc cacccaacag accgttctcc ataaactggc agaagatccg
    27661 tccatccaca gcgggcatcc ccaacaggcg tttcccgtca tgaccgaaca cgcggccggc
    27721 acgttcgcgg ttgagcgagc gagcatctgg ctcttcgacg gcacccagtc gacgctggtt
    27781 ctacaagact tgttcgaggc gacctccaac cgccatagcc agggaaccat gctttctacc
    27841 aaagagtacc cggcctatct tcacgccctt gaaacggagc cctattcctt ggccgcccat
    27901 gcggcacaat ctgatccgcg caccagcgaa ttgacccctt catatctcat cccgcttggc
    27961 atcacctcga tgctggacgc tcccattcgc cgccatggac gtgtggtcgg agtcctctgc
    28021 ctcgaacata ccggcacgcc caagacttgg accagtgagg aggaagcgtt cgccgcctct
    28081 ctcgccgcca tggcgaccct caccttggaa gcagccgacc gccggcaggc gcaggaggcg
    28141 ctggaacaac atgtccgcga gcgcacgggc gaattgcggc gcatgaccgc gcatttgcag
    28201 accatcatcg aagaatctcc tctcgcaatg attgagctgg atcacacagg ccacgtcacg
    28261 acctggaatg ccgcggccac ggccttgttc ggttggacca gggaggaagt tctgggcaag
    28321 gagcttccct atgtgcccgc gggtcaggaa caggcttcga acgagttgtg gacctctgtc
    28381 atgaacggcg gcgcgccgcg caacctcgaa ctctgtcggc agcgaaaaga cgggaccttg
    28441 atcgatgtga acatgtgggg aacgttgctg caaggtcccg gcggccagac gaccgggtcg
    28501 atcggctttt tcatcgacgt cacggagcac aaacaactgg aggaacagct ccggcaagcc
    28561 cataagatgg aggggatcgg cagattggcc ggcggcgtcg ctcacgattt caacaatctg
    28621 ctcaccgtca tcaacgggtg cgccatgctc ctattggaac aggtgcgcgc cgaagacccc
    28681 ctgcatcgat cgctgaccga aattctgacc gccggtcagc gggcggccgc gctcaccaag
    28741 caactcctgg ccttcagccg tcgacaggtc ctgacgttcc aggttttcga tctcaatgag
    28801 gcgctctcct cgatcagctc catgattgaa cgattgatcg gcgaagatat tgccctcgtg
    28861 agagacctca cgtctcagcc ctgcatcatc cgcgcggacc ggggacagat cgatcaggtc
    28921 gtgctgaatc tggcggtgaa cgccaaggac gcgatgccca acggggggac gctcaccatt
    28981 gcgacaagga ccgtcacctt gactgcagac cagccggtgc ctcatcccgc gatgcttccc
    29041 ggccggtacg tgcggctctc cgttcgcgat tccggagagg gaatggaccg gaccaccctc
    29101 tcgcatattt tcgaaccgtt cttcaccaca aaggaggagg gcaaaggaac cggcctcggt
    29161 ctggccacgg tctatggcat cgtcaaacaa agtcagggct ttatcttcgc tgacagcaac
    29221 cctggcgacg gcgccacgtt cgacatgtat ttccccttgg ctcaggctcc atccccggcc
    29281 gctgctccgg ccactccctc gcgccctcac tgtggtcacg aaaccattct cctggtcgaa
    29341 gatcaaacgg ccgtccggat gctgctcacg caggccctct cggactatgg atacacgttg
    29401 atcgaggcct ccagcgggca ggaggcactt cgcctcgtag ccgccgccaa gacgcccatc
    29461 catattctcc tcacggacgt cgtcatgccg cagatgacag gccctgcgct ggccgaacgc
    29521 ctccggcagc aatggcccag ccttcgcgta ctctttatgt cggggtatgc ggaagggagt
    29581 gtgttgccga cgttcctggc agaaccggga accggcttca ttcaaaaacc gtttttaccg
    29641 acggagctcg cccaaaaact tcgcgaacta ctcgattccg ccaagtagcc actcggtgag
    29701 tgacacgggt gcgcgagcgc cacgcccgtg aatcacccgg gcaggatcga ttggaaattt
    29761 tcgatggagt ggaatcgtgc ggaaggaacc ggcgaggctt gagagctcga cttcgagcga
    29821 tgaaagatcc ggcggatgcc gaactgttct gccgccgcga gacattgctc gtcatcatcg
    29881 atatacaaag cccgtgacgg atcgaagccg accagctcct gacaggtcgg ccagtattcg
    29941 gagcgcatct tcagccatcc tacctcgaac gcatccacga tgcggtcgac ctgccggtgt
    30001 agaccggttt tggcagtttt gacctcaacg cccgcccggt gcgcgttcgt gagaatcgtg
    30061 acccgctttt cgagccgccg caacgacagg agaaacgcct cggcatcggg gaggaatcca
    30121 atcatgtggt cgagttctcg atgcatcgcc accacatcga tctgtacctg ctcggtccaa
    30181 taatgtagat cagtccaggc gagttcgccc tctacagaac gatacatgct catcagtcga
    30241 tcccgggctt ccgcgaaggc caatgcatgt ttcgccgcgt accgtctggg cagttcttct
    30301 tcgaaaaaaa agttgtcgaa atggcggtcc aggagcgtgc cgtccatgtc gaggaggaca
    30361 tcgtcgatgt gactccaatc gacgggatga ggcgtcgggt ccgtgcggtg ctgcagcatc
    30421 agttcagtat aacggagacc ggtaagacaa gacgagtcat tgtgttcaac gaaatttcta
    30481 tgttaccgtc gcaggccatg tcacgcacca aacccgctcg tggtcccgtc acaccgctct
    30541 tcacgctgga aggcggtcgc ctgcccatta tcgccctcgt gggacgtccg aatgtgggta
    30601 aatcgaccct cttcaatcgc attctcggca cacgagccgc cattgtggac gatgtgcccg
    30661 gcgtgaccag agatcgcaac tatgctgacg ccacctatcg caaccgccat ttccgcttag
    30721 tcgataccgg cggactcgat ccgacggcct ctgaaggaat gttgtcgctc atccggcaac
    30781 aatctcaact cgccatcgcg gaggccgaca ttctagtcct cgtcctcgat gctcgagccg
    30841 ggctcacgcc gcccgatgaa gaggtggtcg cgacgttgcg cggctccgac aagccggtgt
    30901 tttatgtgat caacaaggtc gatacgccca aggccgatcc gctcgtggcc gacttctatc
    30961 gcctggggca agagcacctg tacccgctct cagcggaaca tggtatcggc gtggccgaat
    31021 tgctcgacga tttgtttccc catatggtcg acccgccggg tgaagaaacc ccgacggaaa
    31081 tcccccgcat tgccatcgtc gggcgtccga acgtcgggaa atccaccctg gtcaattcag
    31141 tactcggcga ggcgcgggtc gtggtcagca acgtgcccgg aaccacccgc gatccggtgg
    31201 actcgattgc cacctttaaa gatcggcaat acgtcctgac cgacacggcc ggtatccgcc
    31261 ggcgaggccg tgtcgaacgc gggatcgagg gctacagtgt ggcgcgctcc cttcgggcca
    31321 tcggccggtc agatgtcgcg gtgcttctgc tcgacgcgga agaaggtgtg acggaacagg
    31381 ataccaagat tgcgggcctc gtcctcaaac aggggcgcgc ctgcattctt gtcgtgaaca
    31441 aatgggatct caagaccgat gacgtggatg ctcgagaagc gtacaaacag gaccttgaac
    31501 gccgctttcc gtttctcgcc tgggcgccgg tgctctttat ctcggcgctg gagcaggatt
    31561 tcttgcgcgg cctcttcgca ttaatcgatc aggtctattt ctccttctgc aaacgggttc
    31621 ccacgggggc gctgaaccag ttttttcagg gtctgttaga agaacacccg ctgccggtga
    31681 ggaaaggcaa gccggccaaa gcgagtaaat cggcgtttgt gacgcaagtg gccacacgtc
    31741 ctcccgcctt tgcgctgttc gtgggacatc ccgacaacat gacgccggcc tatctccgtt
    31801 ttcttgagaa ccaaatccgg aaggaatatc atttttccgg cacccccatc cgtttgatgg
    31861 tccgcaaaaa atagtgcgcg ggagacagca tcgcagaccg tggcgctcct gctacagtcc
    31921 gtcgcatatc ccacttcctg aatgacctac atagaggtcc cgaaggcctt tcaacttgac
    31981 tcatgccgac cagcatgtta ttcgcacagg agacgttgta cgccacgtac gatccggcag
    32041 aaccggccat tcactagtaa tggccggttt ctcccccgct caatcggcgt gaaaccattc
    32101 cacaccgcat cattcgatac acattatggt aactctcgtg aattccgacc aacgattccg
    32161 atgcatttcc ctctccttga gtgcgctgct ttctctgagc tgcctgcccg cagcaggctg
    32221 ggctgaaccc tccgccccgc tcatcgaacc acctccactc gaagagccgg ctgcctcagt
    32281 tgaaccagtt gcaccggagg cgcttccggt tgaaccggaa ccgacccaga ccgtggccac
    32341 tgcgcccgtt tccccttccg tgcaagtatc cggtttcatt tggagatcca agccgggaat
    32401 cgtctttctc caaacaccga tcggacctct ttctctgagt tcaaaaacga cgctgcgcga
    32461 cgtcaaaaat tcgcaaaaga tcacactctg ggttcatggc acgacaacag tcattgatat
    32521 ccgcgagagg ggggccgaca agctggtgca tcggtatgtg acggccatgc ccaatttcac
    32581 ctcccccgaa aaacagacgg tcgcgctctg gacccctgac ggcgagcaat cgttcgccct
    32641 cggcgggctc gcagcgaaaa ttgccagccg ttcagacgaa cagcctttta ccatcgaggt
    32701 ggatggatcc ggcacggttc gcggcctgca tgacgtgcaa tacgatcttc aagtgaatca
    32761 ggttccgcgc acaccatcct ccgtccatct tctcttgaac ggcacggttt cgaagctaaa
    32821 gtcgaactat gtgtttctca aaacccctct gggaatcgtc accgtgagcg ggaaaaccgg
    32881 tgtccgcaac gccaaagtcg ggcaagagat gtcggtgtgg gtgcaggatc ggcacgtggc
    32941 catcgacctg tatcaagacg gtgtgtccac tccatcccgc cgctttctct ccggcccgtt
    33001 gacctatgcg ccggaagggc agagcgcact gatcatgcag ggacccgagg gcgcacagtc
    33061 cattccgctc gcgcaacggc cggcggccct ctcggccgtg aaggaaggct tgccgattac
    33121 ggtcgaactg ggccggcaag gcgaactcct cgacgtccgg cgcgtcaatt agtcatcgcc
    33181 atcaacgtgg gtgtcgaccc ggccgttcgc ttgacagggt ctgaacacca cccgtagact
    33241 cgcttccgcg tatggcatca cggaagaaaa gtccgccggc gtcatctgat gggcacggcc
    33301 tgccccactc cgcatcccat ccattcgacc aactgtatcg agaccatgtc gacgtcatgt
    33361 accgctttgc ctatcggctg tgcggcgaag ccgaagcggc caaagatctg gtccaggaaa
    33421 catttctcaa cgcctatcgc gcctatgatc gctttcgcgg ggatgcgcgc gtgtcgacct
    33481 ggctctatgc catcgcctcc catgcctgtc agcgcatgcg ccgaaaacgc aaaggcgaac
    33541 cggaacgcga gctgtcgctg accgaattcg tgccgacgtc ggaaggcgag tttgccctac
    33601 agataccaat ggagggactg agccctcagg aagcgctcga gaataaggaa ctgcgcgaga
    33661 tcttagaccg ggccatcgcc aaattaccgc gcaagtatcg tatggtcctc gttctgcgcg
    33721 acatggaagg gttgagcgcc aaagaagtgg gcgacatcct cggattgagc gagcgcgcca
    33781 tcaaatcccg gctccatcga gcccgtctgt ttgtcagaaa agaactcagt ggaaaagggc
    33841 tcgcggagga atttcaccac gcggttacca ctgtggatcg atagtttggt atagtcaaag
    33901 aagcagtatg accatggcag accgcgtacg cacatatcca ggcactcgca agcccacccc
    33961 gcatcagggt caccgcaaac ggaattgcgt caaagttctg gaacgccttt cagcctatct
    34021 ggacgacgag ctgtccggcg agatctgcca ggaaatccgc gcgcacctcg gcgactgccc
    34081 gaattgcgaa atattcctcg attcccttcg ccagacggtc cggctgtgcc agcatcggcc
    34141 ctccccctcc ctctctgagc acgatcgagc cgcacttcga cgccagatct taaaggccgc
    34201 cgcgacggtg tgagccatcg tgacgcgtcc gaatcccatc acggctgtgc ctgagccgtc
    34261 ggcaactgat tccctctcac agactcgctc agcgtcgcgg tttcttcacg catgttccag
    34321 cggccttccg atcatggtcc tgctcatcgg caccggcatc ctgctcgccc agccctctga
    34381 cggcctgcaa cgcggccaat ccctgtatcg tgaacattgt atggattgcc acggcaccgc
    34441 ggggaagggc gatggcccca aggctccgtt tttatcgccg cgcccgggga atctcgtctc
    34501 tgcagccacc tctgcaaaga cagacaagga gctcctccgg acgattgctc aaggcaaacc
    34561 ccgcacagcc atgcccggct ggaaggaggc gctcccggag gaagatcaac aagccgtgct
    34621 ccaatacatt cgctccctga ttcaattttc ccgttctccc accgctactc ccccgactcc
    34681 ctgaacacgt gttcttaagc caggcctctt tcgtctgaaa agagccgcgt gatagagtga
    34741 ccttctttta tcctcatcgg aggtccttcg tatgatccgc gggaacctgt gccatcaccg
    34801 acagaccatt gccatccttg ccctggcgct tgcattcggc tcctgggctg gaatcgctga
    34861 atccgcgccg gccgtgggcg gaaagggaca tgccaagacc ggcaccccta aaaccacacc
    34921 ggcgatacaa acagccatac ggtatgctga agcactttcg cagggcgatc gcgtgacagc
    34981 cggccaattg gactttgcct gccagtatcg gcacctcacc gcttcgccga cgaaggtgaa
    35041 acaattcgcg ccgaaagatg atccgtctta cgacgcgtgc tggcagaacc tgaccgacgc
    35101 ctatgccccc atgctcacca ggtcagacat agcgatggat atcatctggc cgagcacggg
    35161 tccgctgctg ttcttcggcg atgacctacc cagagcgcct gcctccgcgt ttgtggcgga
    35221 tgccgtgggt gttgcacctc cgggcagcgg actgcatgtg accgccttga acagccgcac
    35281 gatcccctcc gggtccttca agctggcgcg caccggaaaa gtcctggccg ctccgaccac
    35341 cctggtccaa ctgtccgtgc agtatcagga tcccctcaca tcgcccgtca cctatgctgc
    35401 cgggaacgtg aagtggacga atacgatcaa gcggccgcgg cgagcattga aatccgtcac
    35461 cctgcagtgg gtcgtcttca gcgggctgaa gcaacatggt tttcctggag atacggcggt
    35521 gttcaacctg ccggttcctt cacagccgga agcacccggc atgatgacag acaaaattcc
    35581 cttcacgaca gaaaccagcc gcgcgttacc tgaatccatg gcctggtggg gaccggacga
    35641 ccaaccgggt gtcctgaccg cggcggcggc acgggccgcc atcttcccag acctgcgcga
    35701 ccgggtcgcc ttgctgaacc gtgtcttgat catcgatccc aaacagcccg atgctcttac
    35761 ggtgctgacc cgccatctgt acagcgcgct gctgcgggag gcgagaagca gtcatcaagt
    35821 gccggtcaag gatccggcgc ttggcatcgt gctggatgag ttttattgga acatttacgc
    35881 acaaggcatg cgcctggacc tctccaacgg catggaaatg ggcggcctct cgcaacccac
    35941 gccggcagat tttctttatc gcctcatacc ggctcttcaa accttggccg agatcagacc
    36001 ggaacaactc gacaaccggt ttcgcttggg agtcgcttat cggtggaaca acgatcagat
    36061 tccgatggtc gaaaccttcg aagcgttggt caaggatatt ccggagcatc gcagaacacc
    36121 caaagccgaa gccctcttgc aactggcctg gtctcgtatc aacaaggtgt cctggaaccg
    36181 gattttgcat gacacggaaa ctccgcgagc ctatgccaat gccgaagcct cactggcatt
    36241 tgccgaactt cccttggata agttcctcgc agaatatgcc atggcctaca ccatgatctt
    36301 tctgcccaac tatggggaca aggagaagat gctccatcac ctcaccgaag ccaaacgctg
    36361 gttcgatgaa gtgccgggga aaacgaacga ggcctggcgc tatttcctcc atacaggcct
    36421 actgaaagcg gtgctggatg ctgaccccat ttttgcacct atcctcgcaa cagtccctag
    36481 ccagccgtaa tcatggtcac tgatcggatg gcgaaggcca aagccttcgc catccgattg
    36541 gccgcgccat cactctctcg tcgcatccgc ccctccgtac cttgtcaagc tggacaagct
    36601 gtggtaacga tgacccgaca gcattgaagg ggagtcgcag atggcggaaa catcacatcc
    36661 tggaagcgtg caaagaactg ttggtatcgc ctgcctcacg gtttgctatc tgaatctgac
    36721 ggcctgctct ctctttggcg gaacttctca atcactgacg gtgaattcag atcccccggg
    36781 cgccaatgtc ttgatcaacg gcacctcggc cggcacaacc ccgctgcagt accaggttcc
    36841 acgccgcgga gacctgacag tcgaagtaca aaagcccggg tacacaccgc agacccgagt
    36901 aaccggccgg aaactcagta gtgtcggcat cgtcgatgtc atcggtggtg ccttgttctt
    36961 gctgccgctg ctgggcctca ttgcgccggg cgcctgggaa caagacccgg gcatcatcgg
    37021 catcacactc gaaccggatc accctcaacc cgcgcagcct caataacttc ggacccacga
    37081 ttacgctgta cgatcagacc gtaaggaaca ggactatgat tgacgtcaaa ggttatgccg
    37141 cccgcaccgc aaaatcccga ctggctccgt ttacgttctc acgaagaaac gtgggaaggc
    37201 aggacgtgct tatcggcatc cgctactgcg gcatttgtca ttcggacgtg catcaggcac
    37261 gggatgaatg gggcggctcg atcttcccca tggtacctgg acatgaaatc gtgggcgttg
    37321 tcgaacaggt cggcgcctcc gtgaaacaat tcaaggtggg tcaaatggtt ggcgtgggat
    37381 gcttcgtcga ttcctgccga acctgcccgc aatgcaaaaa aggcgaagaa cagtactgcg
    37441 aaggccacat cagctttacc tacaatggga aggagcggga cggcgtcacg ccgacctatg
    37501 gcggctactc caccaaagtg gtggtcgatc agcgctacgt gcttcgcatc cctaagcaat
    37561 tgcgtcccga ggaagccgcc ccgcttctct gcgccggcat cacgacctac tcgccgttac
    37621 accattgggg agtgggaaag gggcaccgcc tggcgattgt cgggctggga gggctggggc
    37681 acatggcggt caaaatcgga aaagccttgg gcgctcacgt gaccgtgctg agtcattccg
    37741 acaaaaagcg gcgcgatgcc aaacgattgg gcgccaaaga tttttacaag acctctgacc
    37801 ccaaaacctt caccaccctc gccaaacaat tcgacttcat tctggacacc gtatcggcgc
    37861 cccatgacct ggatgcacag ctggaattgc tcaaaaccga cggcaccatg attctcgtcg
    37921 gggtgccgga caaaccggcg caactggggg cttttccgtt gatcctcaaa cggcggaggc
    37981 tggtgggctc attgatcggc ggcattcagg aaacacagga gatgctggat ttctgcgcga
    38041 agcacaaatg cggcgccgac gtggaagtca tcccgattca gaaggtgaat gaagcctacg
    38101 atcgcctcgt tcgcggggac gtgcggtacc gcttcgtcat cgacatgggt tccctcacct
    38161 aacgcaccac gtcttcttcg ttgcacgcga catccatcgt ccgatgtcgc ctcggcatgc
    38221 ccagctatca gcagcacgga gccggtacac gtcaacagtc tcaatgtgtt gaatgtgagt
    38281 ctaccgagga gagaggcagg ccgggcggct gtagccgccc ggcctttcct caaagacaca
    38341 ggaacttact tggcaggctc aaccaacggc atcaaaggaa gctgtttcgc gtccttgtcc
    38401 accttcagca gtgcgatcgc gccccgaagg gcatccgtca aagagtgcgt gacgatggga
    38461 tacacgccgt cattgccgtc caacaccata tcgagaatgg ccgctcccgc cggcgggatg
    38521 agttgcgttt gtactccgcg cgtatggttg gcggggttgc cgctttccca gacatcatcc
    38581 cagatttcgg caatcggatg gaccgcggac acattgttcg gtccggcatt gacgaaatag
    38641 aaccggacgc gctcacccgg cttggcttct aaaaaggcgc ctcccgcctt gctatgcacg
    38701 gggtcatacc ggaacaccgc cccgttaaaa acgacgtgct gatatttccg gtcgaacatg
    38761 ccgtcgacat cgtcaggatc tttgaacagc tcgctctgca ccaacacata ttctcgatcc
    38821 gccttcggca tcgccttggg atccttggga tcgacgatga tggcgcccat catcccgcgc
    38881 gcgatgtgct ggatcatggg agacgcgcca cagtgatagg caaacacgcc cggccatttg
    38941 gccttgaacc gatattgcaa agattcaccc ggccgcactt ccttgtagtg ctccaggaaa
    39001 ttggtttcgg cggcgtgaaa atccatggag tgaggccggc cgttggtctc cggattcgtg
    39061 agcgagaaaa caacttcatc gccttcctgc acccgaatca ccgggcccgg gaactgcccg
    39121 ttgaatgtcc aggcatcata tgtatgtccc tcgccgtcga taacgagctt ggtttccacg
    39181 gccgtcatgg acacttcgac gactttcgcg aacgcggatt cgaccggttg catggcgagg
    39241 cctgacattg ccgccaaccc taaaaccgtg cggatcacag attgacgaaa gcggatattg
    39301 cgtactgtca taatgctgcg gctcctcctg tgaaagtgat tggtctcggt tcacccagaa
    39361 ccgtggtgag gataccgcgc aacgcgacaa atcaagtatg acatttgtca tgttttaaaa
    39421 atgtcgcgcc ggctggcgcc gatcgcctat cgggatcttc ggccagggca ttaccgcttg
    39481 gcagctaaat ccgaaaaata atgggcgaag gccttgatgc cgccggaggc ctgcccccaa
    39541 tcgaagtact cattcggcgc atgatatcca tgctcgggga ggctgagacc cataaacagg
    39601 atcggcactt tccacgcctt ttgcatcgtc accaccgcac cgatcgaacc gccttcacgg
    39661 atgaaggccg gctccttgcc gaatccggcc aggacggcac gcttaaccgc ttcgacatag
    39721 ggaccgtcga acaccccacg gaacggatgc aacataccct ctcgctccac cttcaccgcg
    39781 ggattcagtt tcgccacgta gtgcttcaac agggcaaaga tcttctcagg cgtttgaccg
    39841 gggaccagtc gcatgctgat tttcagttcg ccatggccgg gcacgatagt cttcacgccg
    39901 gggccatgat aaccacccgt aaggccatgc acttcgaatg tcggggcggc ccaaattctc
    39961 cgcatgacct ccgccgggtc gtgcgtgcgc aaggtcttga acccgtacgc ttgcttgaac
    40021 ttgctgacct ggaaacccga tttcaagaaa ctcgcgatct ccgccttcgt tggcggcacg
    40081 acctcgtcgt agaatccggg gattttcacc ttaccggtct tggcatcgac acaggcatgg
    40141 gcaatctcca tcaactcgac gagcggattc cgggccgccc cgccggtcac gccggagtgc
    40201 gcatccttgg ttccggttcg aagggtcagc cgtgcgccga gtaatcctcg caatccatac
    40261 ggcatcgccg gttgattctt ggagagccag atggtgtccg aaatgaccac cgaatccgga
    40321 gccggaatcg ccgcccggtt cttgatcgcg gaggcaaaac tgggactgcc gttttcttct
    40381 tccagttccc agagaaaccg cacattgatc gggacccctt gatcaatcgc atatcgcgcg
    40441 ccgaacaggg ccgcgagggc cggccccttg tcatctgtcg cccctcgccc acggtagatg
    40501 ccgtgatcat tctggaaggc gaagggagcc tgcttccatt ccggttcctg cgccggctgc
    40561 acgtccatat gattgtagac cgtcacggta ggatagtgag gatcgaccgt ccagccgcct
    40621 gagacgaccg ggtacccggg cgtttccacg atctgcgctt ccgcaccagc cgcccgcaga
    40681 tactgtgtcg ccagttcggc catgcgccgc acatccggcg catggcgggg gtccatgctg
    40741 atggacggaa tctcgaccgc ctgcccgagc atgtcttcat actgcggacg gatatccttt
    40801 atgtatgtct ctatctgccg ttgcacgtcg ctcattgatg ctcctccccg atctcagtgg
    40861 cagtgcccga attccctcac atcatttctc tgcatcacac tcactacgta ggacccaatc
    40921 ccagtcacgg cgattatatc cagtcggaac aggaaagcct atgttggcga cgatggcgag
    40981 aaagcatgta gaatgaaacc gatgcacgat aacacttctc accaagcact tcgtatgggc
    41041 acagcactgt tggcttgcct ggcattgtgt ctttcaggcg gcaacagagt attcgcgcaa
    41101 gaagccgtga ccgtgcagga agtgctcgct gatcccgcgc tatttcattt gcgacaaatc
    41161 accatcaagg gcaccgtgcg tcaggtccaa ccgctcgatc cctacgagat cccggccggc
    41221 tcaacctgtt acggcggcta tctgttcagc ctggaagacg acacggccac cctgtccatc
    41281 gccgtacccg gactatgcgg cgtgccgatg gtgaaagatc cggacgtgga agacggtgcc
    41341 cgcgtcgtgg tcgaggccac ggtccaagct cccagccatg gagggtatgc cttgagcttc
    41401 aaaggcgcca aaatcgccat ggatcaagaa ggcgttgtac aagccattgc aaaccggatc
    41461 acgccattgg tggaataaac gatgacgact cgtccacttg aacagactgc ccgcgtcgcg
    41521 gcccccgccc atacgcgcgt cggctggatt ggcaccggcg tgatgggcgc ctccatgtgc
    41581 cgtcacctcc aggctgccca ctatcgcctt acgctgttta cacgcaaccg cggcaaagcc
    41641 tcagcgattc tcgccaaagg agcaacctgg gccgattctc ctcgcgcggt ggccgagcag
    41701 accgatatcc tcatcaccat ggtgggattt cctcgggatg tgcgccaggt gtacttcggg
    41761 gaccatggtg ttctggccgg cgccggcgcg ggcatggtgc tggtcgatat gaccaccacc
    41821 gaaccctcac tcgcacagga gattgcacag gcggcccaat cgcgcggagt gtttgccgtc
    41881 gatgcgccgg tctccggagg ggatattggg gcgcgcgacg caacgttatc gattatggtc
    41941 ggtggtgatg ccagcgccgt tcaaacgatc atgccgctgc tcgaatgtct gggcaaaaag
    42001 atcgttcacc aaggccattc gggagccggt caacacacga agctctgcaa tcagattgtg
    42061 atcgccggca cgatgatcgg cgtctgtgaa agcctgctgt acggctataa ggccggactc
    42121 gaattgccgc gcatgctgga atcgatccgc ggcggcgccg ctgcctgctg gacgctggac
    42181 aatttgacgc cccgcattct ggctcgcaac ttcgaccccg gcttcttcgt cgaacacttc
    42241 atcaaagaca tgggcatcgc tctcgaggaa gcacggcgcc ggcagctcac ccttccagga
    42301 ctcacgctcg cacatcaact gtacgagaag gtggaggcgc tgggccatgg ccgctccgga
    42361 acgcacgcat tgatgctggc gctggaagcc ctttcaaaca tcgatccaac cagaccctcg
    42421 acctcactct aatcacagga gccgcatgag aacgggtatg catcctcttg ttctggcact
    42481 tatcctcacc atcacctgca ccgcctgcac cgaagcccgt ggcctgaacc ggcacatctt
    42541 gcaggaatcc tttcatgatc atccggacgt aacgactgat cgcgccatcg ccgccaccat
    42601 ggcaacccag gcaacattgc cgacgccgta tcgcctcgcc atttatttca aacatgagga
    42661 tttccccagc cgtcccgcct tgcaacgagt ggactgggtc agttccgacg gcgcccgcgt
    42721 gcaacggtcg ctcgaaccgg tacaggaaga aggcattctt caacgaacct tcttgctcgc
    42781 caattcaacc gtgcaaggca atacccttcg tgacatacgg ctcgccgcgg cccgctacaa
    42841 cgccgatgcg attcttatca tcgacggggc ctcggccttt gaacggtaca acaacggcca
    42901 cgcctggtgg tatgccaccg gcatcggcgc ctactttgcc cacggcactg aatgccatgc
    42961 cctattcatg atggagggga cattgtggga tgtccgaaca ggctatctct acggcacgca
    43021 aacggcggaa ggagaaacaa cgctcatcgg tcccgccccc tcactcgacg acaaagccgc
    43081 ggtggccgaa gcgaaggatg tggcgatggc ccgcttcggc agagaactcg ccgatacatt
    43141 gcggctgctg aaggaaaagc agaaggcaat accgtgaacg ccctcctcag tttctgcttc
    43201 cagttgtgac tgagatgctg cctgcgtatg gtcgcgaagc agttgagacc gaaatccgcc
    43261 gcggttgatc cgaaccagca acagctcgca actacacctc ggtctcctgg gcgctcaaaa
    43321 tggctttcgt gcgaggccgc aggacgcgag ggactgaggc gtactctctg cagtacgtcg
    43381 aggtcacgag tgcgccgaga acgaagcccg aagccatttt cagcgcccgc tcacggacta
    43441 tcgaacgtcc atagaattcc gagcaacacc aggtgctgat tcggtgtgag actcagcacg
    43501 ccgggttgca cctctccacg ccgaaagaat ccgcctcctg ctccggtcga ttcgtcccat
    43561 cgatgttcga ggcgcaggat ggtattcgtc catttgtagg gaatcttgta ttccacggtc
    43621 gacgtcaccg ctttcacgaa ttgctccgat cccgtccacc gcccgtttcg gtcccagtag
    43681 aactccggcc tcgccaccag agtccagggg ccggcgacat gccaccgggc taccatattt
    43741 ccgccggtca caaaggcccg cggatggccg ggacgatcgg cgatgtt
//
LOCUS       DAOQDS010000035        43038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_285_length_43038_cov_19.522951, whole genome shotgun sequence.
ACCESSION   DAOQDS010000035 DAOQDS010000000
VERSION     DAOQDS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 43038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43038
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_285_length_43038_cov_19.522951"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PK589_10325"
     CDS             complement(<1..287)
                     /locus_tag="PK589_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20040.1"
                     /translation="MTNLRRAVSILLFLSVLLPTAPGWSMDPLPIEPDLNSRLDELYD
                     HESRMFIMLYSLHGDGKVDYVTGRLVQEYTRSNYGNPVYYTEQFPLFYWWN"
     gene            445..1776
                     /gene="mtaB"
                     /locus_tag="PK589_10330"
     CDS             445..1776
                     /gene="mtaB"
                     /locus_tag="PK589_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNJ20041.1"
                     /translation="MPRASLHTLGCRLSQSETAMLGNSLTRQGYRLVEYGEETDLLVL
                     NTCSVTENAEKDCRYAVRKTLRHSPHAFVAVTGCYAQTGAARLQTVPGIDLIVGTQYK
                     MNLPDYLPAPTQLRKQPEPELRHSRTIDREDFVLPGTAYSDSTRALLKVQDGCDFMCS
                     FCLIPFARGRERSRVADDVLREARELAIHGYRELVLTGVNIGQYSYQGMELVDLLQEL
                     EAIPEVVRIRISSIEPTTLPTRLLQHMASSTKLCHYLHLPLQSGDDGILQAMNRRYGI
                     REYEELVEQAQALMPDLGLGTDLMVGFPGEDEQAFANTMAMAARLPFSYFHVFSYSSR
                     PGTPAARLEPQTPGTIIRQRSKTLAELSRTKALAFYQRQIGRTVSVLFEQGEHDGFRT
                     GTTANFTRVAVPADAVAAGSIHPVTITGIMDGLAYGHLASPSVEPTYRPLL"
     gene            1773..3131
                     /gene="miaB"
                     /locus_tag="PK589_10335"
     CDS             1773..3131
                     /gene="miaB"
                     /locus_tag="PK589_10335"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNJ20042.1"
                     /translation="MTQPNKPHTVHIETFGCQMNEYDSELVRTLLRKAGFEFTEDRER
                     ADVMLMNTCAIRENAHNKVYGHLAELKAVKEQRPLVVGVLGCMAQNLKEELTEKQPLV
                     DVLVGPDGYRQLPGLLTNALNAEEQGLSRRGLAVDLSEYETYDDILPERDGGCNAWIA
                     IMRGCDNFCSFCVVPYTRGRERSRDPQGILREVEVSVEAGHTQITLLGQNVNSYRYDD
                     WDFARLILAVAEVPGVQRVRFTSPHPKDFPAALLDAVAGHPNICKHIHLPLQSGNDRI
                     LELMNRTYSRKEYLDLATTIRRRHPGIALTTDIICGFCSETEEEFLDTFRVVEDVQYH
                     SAYVFKYSERKNTIAARKFPDDIPEAVKGERVSRLVDLQRPVTARINRELIGQTLSVM
                     VEGDSKRSADQWMGRTDTGIYVIWNKSDAPALLGSLQPVTILDGSAAVLMGRGEPGAT
                     LS"
     gene            3593..3724
                     /locus_tag="PK589_10340"
     CDS             3593..3724
                     /locus_tag="PK589_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20043.1"
                     /translation="MKKDIIQTHVEEAPKGISLETIIAVGVFGWMALGVVMMGLGIW"
     gene            3811..4848
                     /locus_tag="PK589_10345"
     CDS             3811..4848
                     /locus_tag="PK589_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20044.1"
                     /translation="MLIHGSHSFLMHRLNSGILGLTLMGALASGCSSTAASVQQPAAM
                     QPQGSVRLEEVADWEFEASHPSSIDAATLDSLLRAVVIAEAQTDMSNLPVDGSKPMTV
                     FSDEDVRYLAPLLAQALSQAQPEYVVAFRLSSSAGSGSEPTAGTLYVKNDRLYLTLSE
                     HHGSLAKTESTWLGSGRAARIVSVMPASAGQLEQALPAVIQGRQQMKSLAIDYHQFAK
                     KPEPAPMTAATTAPAPQPVQALSVRNVPAVAPDQPAKQQSAPAPVMDKVASNQAPAED
                     ERLNETLQELQDTKDAMARKDAKISALRRDLESMRMQLETKDKELRAVKNTQQQQPKR
                     ETRNKAELTVR"
     gene            complement(4864..5580)
                     /locus_tag="PK589_10350"
     CDS             complement(4864..5580)
                     /locus_tag="PK589_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNJ20045.1"
                     /translation="MKVVLDIETVQAPREEWARIAGRQLTSGEVTFAESGGDLFAVGE
                     AEAQQRLDDELYEKSSFDGTFSKIVCIGLLEFTDTLEPRGAISWYGGNEQALLRAFWS
                     HLGQSRPSLFITHNGLNFDLPFIKKRSIIQQVKPSMEINLAKFRAEPVYDTMAIWSNW
                     DNRGWVKLDVLARALNVESKSGSGSQVAQMWADGQGKEIALYCLQDTYVTYGCYCRMN
                     FRPPISREIVLLKPELVDVG"
     gene            complement(5587..6015)
                     /gene="mutT"
                     /locus_tag="PK589_10355"
     CDS             complement(5587..6015)
                     /gene="mutT"
                     /locus_tag="PK589_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HNJ20046.1"
                     /translation="MMVQRLDTGKVAMQVIEVAAGIIAHEGRYLIARRKAGAHLGGLW
                     EFPGGKRETGETLEECLHRELWEELQVRVSAPTPFQIVRHEYPEKVVELHFFRCRIEA
                     GVAIAIDCAELRWVYPHEMASFEFPPADQPVIAALQQRKV"
     gene            complement(6063..6782)
                     /locus_tag="PK589_10360"
     CDS             complement(6063..6782)
                     /locus_tag="PK589_10360"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNJ20047.1"
                     /translation="MPTKPRTQKPSRRKKQTSTPALPLARGQKQRFQNRLLKWYKEYG
                     RDLPWRKTSDPYHILVSEVMLQQTQVDRVIPKYHEFLERYPSFAELADAPVAEVKQTW
                     YPLGYNIRPERLHSIARETVARYGGQLPSDADELLSFKGIGRYTAGAIRSFAFNEDAP
                     ILDTNVIRVLHRVFIAEGDPKGQKALLWELSEALIPRGKGYDFNQAIMDFGATICTAR
                     NPYCLLCPMKPLCKTYPFGEK"
     gene            complement(6935..7300)
                     /locus_tag="PK589_10365"
     CDS             complement(6935..7300)
                     /locus_tag="PK589_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20048.1"
                     /translation="MKASFSIYALAAIGMLGAAVAFANPALLPQHPGYPMGKATDPVN
                     GQALANDPGRANGGGESALAGSAAFDDRHVSQKLSINDQNQRLLEKPGAGILPKVQGP
                     NIVIDPPVKEGTKVQASPQ"
     gene            7858..8181
                     /locus_tag="PK589_10370"
     CDS             7858..8181
                     /locus_tag="PK589_10370"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ20049.1"
                     /translation="MSMTVHYRVSLSADSTTTGEGRLLDLSLEGCRIECDRHLPVNTY
                     LSLRLQISPNEMPILVDLAAVRWTRGTHCGVHFLSVQPLQSARLQTFLAAASPRKEPP
                     TPGAG"
     gene            8313..8930
                     /locus_tag="PK589_10375"
     CDS             8313..8930
                     /locus_tag="PK589_10375"
                     /inference="COORDINATES: protein motif:HMM:NF038013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HNJ20050.1"
                     /translation="MNEDHLSRRIDVLAIGLFGLAVGALTLGVAQLGWIQQKNMVGAL
                     VIALIFGGVVQLLAGITDIRYNEQLGGTALTMYGFLWITLCTVKLVSASSTVQFDAVL
                     YAPINLVYAVFSGVMIFLTAYRNLTLSALHVVITLTFLATTFARLDFISELLPGWGHI
                     IVGLMAFYHAVGSLTQAFTGKSILPLGPPLLFHTPKHVHSIAKVV"
     gene            complement(9058..9777)
                     /locus_tag="PK589_10380"
     CDS             complement(9058..9777)
                     /locus_tag="PK589_10380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ20051.1"
                     /translation="MKRLDGKVSVVTGSSSGIGKAIALRFAQEGATVVVAARRFCKCE
                     ETVAQIESTGGRAVALQTDIADESQVEQLMAETIRRYQRLDILINNAGIFGGRRLAET
                     STEAFDEVMRTNVRGTFFCCRAAFGLMKKQGGGTIINMSSVAGVQAWSGTGTYSASKH
                     AIMALTKSLADEGRAYRIKVSALCPGGVADELVDATPEARAASEKIDPFDIAEAALYL
                     ASLGPQAVVHQLVVDRLGADW"
     assembly_gap    10009..10108
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10109..13417)
                     /locus_tag="PK589_10385"
     CDS             complement(<10109..13417)
                     /locus_tag="PK589_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ20052.1"
                     /translation="MKLLKLLLQAYGPFTGKVLDFSTGPANLHVIYGPNEAGKSSALR
                     AMMDLRFGIPVRSADDFLHAAGDLRIGGIFAAINGEAIGFVRRKGKGTTLARLNVLTE
                     QPDQSLVMEPGHERGLTGGLERAEFEAMFGLTHARLREGGQVLLRGEGDLGSALFEAS
                     AGTSGIATLLAALETDAKKLYSPHGRAQNAVINEARRRLDEQRQAWREAQTKPAEWQS
                     LYRAHEAAAATLSDVSMTLERLRRRENELIELRTVGPLLRTHDAATALLASFAGVPDL
                     PEQAREARLAALQELNHAEHSLREAEVEAERCARALGALTLEPLVLEHAAAIERFVSG
                     VAAAARYRIEVQQQDAVIAALDADLGLAIARLAPGREVRDVLHAVPSAADRLVLDDHL
                     MQVSRLEDRLEHIRQRATALDQALASSHQESWLPIDPGSRQQLMTAVRHAQSLGDVVR
                     RQKELDQQRREMDLLLEQALADVGLASDQALRRAQPLLDAQIARTRQELNDLDEQIRK
                     LRDEQELVGRDLDGQRLRLRQLAAEGEIVTAETLRQARAKRDDRWKAIREAYIDRTQT
                     LDQLALGFDTARATPEMFEAALSEADRQADLLRADAKRAAGLEECTGRIEQMEGRRSD
                     IERQIARLSAGRGATLDAWAQRLKDAGLPDLDPEPLREWQGRRADALQIAERVAGLRA
                     ASDRLQEETNNAVAGVRAALLKLGHPVSEANPVDVTLLPSLIEQAVAYDSKAVEAEAA
                     HNAKEQAATVQRIERQQLDRSMAEMEAELRRHRSTLQAWHARLFLASDTIPDAIKVRL
                     DELDGAARQAAALGEAKQRRAQVQAILDDLAAQSKDVAAVLDIPAPQMVEDFADRLRG
                     RLAAAKAAEQEQATLLRDQRKAEEKRRGAASDRAAQQTILARLCAVAGVTGLEDLPQR
                     EEAASRKREASASHTHVRQQIAAASSLSVEELRQRLSGRDAVTIETEREQCRSEITRC
                     EEEQAAARRAEEQARLALNAIDSSDRAARAREAMESAAARYRSAIRPWARLKLARALL
                     QEALNRFRERAQAPMITKASAYFALMTDGDYERLISDDTGGQPRLCALRQGGTSVAVE
                     EMSEGTADQLYLALR"
     gene            complement(13414..14673)
                     /locus_tag="PK589_10390"
     CDS             complement(13414..14673)
                     /locus_tag="PK589_10390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HNJ20053.1"
                     /translation="MRFIHAADLHIDSPLRGLDRYDGAPVERLRTATRSAFERLIDTA
                     ISEHVDFLLLAGDIYDCDWQDFHTGLFFRGQMVRLEREGIRVFIVQGNHDAQGVISKQ
                     LVLPPNVTVFSSRAAHTIRLDDRSVAIHGRSFPERAVDEDLVPSYPPPVPGFFNIGLL
                     HTSLTGRAGHDTYAPTDLPTLNHKGYDYWALGHVHAREVIAERPHIVFPGNLQGRHAK
                     ETGPKGCELVTVEAGRIESEFVALDVVRWNQLIVSLDGVQRLEALGEAFRHALEPVLI
                     GAMDRLHAVRVTLTGSTELHRLEASQPGTLAAAMYGAAQDIGEAEVWIEQVRLDLTTP
                     LDRVQTAQRQDAVGELVRLVDSVAGDESELQRWAQVELGDLLGSLPQEVAAGDVPKLD
                     DRVELTQLLMEAEATVLARLTDLGAEA"
     gene            15126..15713
                     /locus_tag="PK589_10395"
     CDS             15126..15713
                     /locus_tag="PK589_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ20054.1"
                     /translation="MSLESTPSTALSPEAIAQLVQGHKQFLAFLERRVESRAVAEDIL
                     QSAFARGLERGAGVDEEKVVAWFYRVLRNAVIDHYRQRSSAERAVEAWTREFPDVQEP
                     AAELRQEICQCVSGLLETLKPEYREALQIVDLQEGKLKDLAQQSGITAENAAVRVHRA
                     RAALRRQIEKACGTCSVHGCLDCSCEASGGSTCGA"
     assembly_gap    15727..15826
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15827..16036
                     /locus_tag="PK589_10400"
     CDS             <15827..16036
                     /locus_tag="PK589_10400"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNJ20055.1"
                     /translation="MAQRLFDKTKWLASNVENLRHEPVAADLPGIHKYAVGDWRIFYS
                     IDRSEQMVDIHYIQHRPQAGRTPGA"
     gene            16039..16344
                     /locus_tag="PK589_10405"
     CDS             16039..16344
                     /locus_tag="PK589_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HNJ20056.1"
                     /translation="MLTLTQKAVDRLRALLLEHPEDPVVRIAVRDVNEQRLSLAITLE
                     AAVREEDVVEQIEGLTIAFDRSSAHRTGGMTVDYQAGRFSFAHPPANELGLIMPGRN"
     gene            complement(16413..16955)
                     /locus_tag="PK589_10410"
     CDS             complement(16413..16955)
                     /locus_tag="PK589_10410"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNJ20057.1"
                     /translation="MKFCSECGAGLSKKIPPGDNLPRFVCETCQVVHYINPKIVAGCI
                     PEWEDKILLCRRAIEPRIGHWTFPAGFMEIGESTEQAAVRETFEEAHAEVEITSLYAV
                     LSLPRISQVHMIFRGTMRTPEFKPGTESLDVQLFTLDAIPWDDLAFPVIQDALERYVA
                     DVARGTFTMHFGSVFPRMKS"
     gene            16990..18183
                     /locus_tag="PK589_10415"
     CDS             16990..18183
                     /locus_tag="PK589_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNJ20058.1"
                     /translation="MDSTIQAFFAPCPRGLEAVLAEELTMLGATAVKATAGGVAFRGT
                     LALCYRVNLQSRIASRILLRIAEGAYRDEHDVYDAAYAIRWQDWFTPQQRIKVKVSAH
                     RCPLKSLDFVTLRVKDAVCDRFQYARGKRPSVDTHAPDILIAVYLDGTQCTFYLDTSG
                     DPLFKRGWRKSSGEAPIRENLAAGILRLSKWTPNVLLYDPMCGSGTFVVEAALMAGRI
                     APGIGRGFAFEKLLSFDAPVLNDLRAELQAAEIPVKSGLIHAADQSAQAITSIRANLA
                     LAGLTEAVTLQQGDVLQLPAPAEAGLLVTNPPYGHRMGDDETLKTFYPRFGDHLKKHF
                     CGWTVQIFTADMKLPGLLRLAPSRRVPLFNGAIECRLFEFRMVAGSLRKPKPPAQSSP
                     GHGHH"
     gene            18297..19823
                     /locus_tag="PK589_10420"
     CDS             18297..19823
                     /locus_tag="PK589_10420"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNJ20059.1"
                     /translation="MSAAHAPLEAPQQEHDLSLSRLIVVSNREPYEHRLVKDHLIWER
                     TSGGLISALDPMMRRLGGTWIAWGSGKADRDVVDEDMVVEVPPDAPTYRLRRVWLENG
                     EVKGGYQGYANQVLWPLCHLTLDRVAYRKAFWHAYQAMNARFADVVLGELAEKPGFVW
                     VHDFHLALLPGLIKASLPNQPVAIFWHTPWPGPDAFRILPERRELLESLLAADLVMFQ
                     TESFLHCFLECAKEFLGTDVLSDEHHIDYKGHCTRLVVRPISINYQSWAERAQSPQVT
                     RAMDVLRNLHIFQPTVRIGLGVDRLDYTKGLLKRLWAIDAFFQQYPQYRGHFTFIQIA
                     VPTRGDVEAYRSYRELIRETVNEINMRYGTISHPGSPQASRWRPIEFREGRIGMDTLA
                     AYYRMADLALVSSVYDGMNLVAKEYVASQVEEKGVLLVSHLAGAAEELTDAVVINPYD
                     PEGVADSIRQALEMPFGERRERMRRMRAYLEAHDIRAWADDCLRDAGILPQQDSPALE
                     "
     gene            19744..21873
                     /locus_tag="PK589_10425"
     CDS             19744..21873
                     /locus_tag="PK589_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20060.1"
                     /translation="MTFAPGLMTACATQESFHSRTVPPLNNRRLPATTDPKIPLRRHL
                     PLFLFLSLSMLLNGCTQLSKLFAVTPSPDQQVDRHQQAIVSRLAPVIEAELQDNLNVM
                     LPTVRKSLADQTNKVAKFKRDLVLRSLSDPWAGLTSIEQTSLVAATAAESGIEELPAI
                     IDILEEGMERSGAPFKPLNFPATLAPEDILGFIMEALEDAHQDREQALRHLSPQDRDF
                     LFTQARPLAEQFLPQIIPPARLAEAEAAGRYAALLMQQVDYASLIAAAQRLARLGNRK
                     LLQQLDIALHHRKPVTQTVPGITGDLLLVQQTSYGLLVIGGHGPNTYDLDKGAALIID
                     LGGNDTYRGLIGASPNSDLGNSVVIDLSGNDLYQPAPLGLATGRAGVGILIDHSGDDT
                     YRLAPGSGGAGFAGLGILYDGEGQDVYEGSRFTQGAAFGGLGLLVDRAGDDRYTSFGY
                     ALGFGGPLGVGALIDVSGNDSYECGGKFPSAYNEADSPHARPQDPAFQYDCFGLGTGA
                     GLRIYSKQPAQRAQSLAGGWGLLVDLAGRDRYRSANFSQGHGYFFGLGVKLDLAGDDE
                     HQAARYGIGTAAHFGVGLSLDYQGKDRYGSKGPFYNGGTAWDGSVALAVDGGPDSDLY
                     DLPASTGLGMADLGGLGLFIEEGGADQYFIGRGLGQGADTSIGAFFDLEGRDDYTSVP
                     LPNGDARPERLNRKTYLENTGSLFVDR"
     gene            22011..24407
                     /gene="lon"
                     /locus_tag="PK589_10430"
     CDS             22011..24407
                     /gene="lon"
                     /locus_tag="PK589_10430"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNJ20061.1"
                     /translation="MTDANAPILTNLPILPLKRTVLFPGTMMPLTVGRDRSIAAVEAA
                     LKTEDKTLLVVAQRDAQTDQPTLEDLYTIGTKAVIKQTARTPEGHYNILIQGLERFVL
                     LKLDQVDPYLQARVKQLPPPSERSTEVEALHRAILDIITELPKLIQTPGVHEAVAALG
                     TEEDPVVLAYRIASLLNLTLDGEQQLLEASTRADLLRGLYAALSREVQILQLRDKITS
                     EAREKLGKTQREYLLREQLKTIQQELGESNGEEDEVAALRKKVQDADLPEHVRKETDR
                     ELTRLAKTPSASPEHQVIRSYLELVLELPWNKSSEEGLDLAHVRKILNEDHYGIKEVK
                     ERIVEHLAVLKLNPSAKAPILCLVGPPGVGKTSLGQSIAKSMGRTFERFSLGGLHDEG
                     ELRGHRRTYVGALPGRIIQAMRRAGVNNPVIMLDEVDKLGRDFRGDPAAALLEILDPA
                     QNHTFRDHYLDLPFDLSKVFFITTANTLETLSQPLLDRMEIIRVNGYSEREKREIALR
                     YLWPRRLKEAGLKAEEVNLPDSVLDHIIGRYTRESGVRQLEQMLGRITRKVAVTFADR
                     PSDSPAAPVEITQSLLDEWLGQERFQPEEARKNLPAGVATGMAWTPTGGDVLYIETTL
                     LPGSHELTLTGQLGDVMQESARAARSYLWSHAESMGLDISRFKRNGVHIHVPSGAIPK
                     DGPSAGITMATALASAYVGKPVRSDTAMTGEISLTGLVLPVGGIKEKVLAAHRAGIRR
                     IILPKANEKDLKEVPQEVRDELTVIPVERIEEVLPAAFNQDAASSPEKHEPMATSTAS
                     "
     gene            24590..25009
                     /gene="msrB"
                     /locus_tag="PK589_10435"
     CDS             24590..25009
                     /gene="msrB"
                     /locus_tag="PK589_10435"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNJ20062.1"
                     /translation="MPSNIEIGPIVKVNKTDDEWKKLLPAAAYRVLRHEDTERAFTSP
                     LHDNHQAGIYHCAGCDLAAYSSDHKFDSGTGWPSFWQPVDPKVVETRTDTKFFMTRVE
                     VHCARCGGHQGHIFEDGPKPTGLRYCINGVSLKFVPA"
     gene            25130..>25688
                     /locus_tag="PK589_10440"
     CDS             25130..>25688
                     /locus_tag="PK589_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20063.1"
                     /translation="MASLPSLRSTMAALWLGVTLLGCSQSSDVISNNLNACLIVTEAE
                     VEFAIGTPVTPGLRQNDRQCLYQAKRNPQETVVVELNAGPDGDRKTLFQDQRSKAGNQ
                     PVPGVGDGAFTLRSPIGGIQLTFLKSDALVTLSLTSPKQANPQAAVTNLAKVAANRLG
                     APIRTTAAAAEPGITGTPSLWAGEWY"
     assembly_gap    25689..25788
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25789..26448
                     /locus_tag="PK589_10445"
     CDS             <25789..26448
                     /locus_tag="PK589_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20064.1"
                     /translation="AAEPGITGTPSLWAGEWYGCMPVGMMYGKGHLSLNERGTWSMTT
                     AVVMPGTIIADRGRWEAESYQEILHGTYKVAGSDRFSTTGILSVTWDKLAKNQGPTKF
                     DPLLWQSFTAIPRKVNVKRLPAVEPSLVGTWEGSSKFVDRQEDFVWTITAANVSEFFK
                     ATSQTGRIEQEQDRFRLVVPQSKTPPLSVRVLAQDKMELTDTSGTVSQWNRNEKLLSR
                     C"
     gene            26539..26946
                     /locus_tag="PK589_10450"
     CDS             26539..26946
                     /locus_tag="PK589_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20065.1"
                     /translation="MRTWLIAIIFLAGPLLPAAGAPVGAAADRPTPKASELLDEIIPK
                     ELFRHLYSEALSTLHEYIEMEGKLPGESSTHQQGGEFRLKLFPHGKSRSQEHLSAEGS
                     FRVAPEADQPEFTLRFKSFKKASPPARTNDDVI"
     assembly_gap    27698..27797
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27798..28783)
                     /gene="clpX"
                     /locus_tag="PK589_10455"
     CDS             complement(<27798..28783)
                     /gene="clpX"
                     /locus_tag="PK589_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNJ20066.1"
                     /translation="MFWDTRHSTQKPDRRCSFCGKPQDAGRTLVTNSEPFACTSCRNP
                     AAVSICEECIQRCSLSLAKNKTPGSQSSVSPLPLVIPKPTDIKAKLDEYVIGQDRAKK
                     VLSVAVHNHYKRIVNRERLKHVDLEKGNILMIGSTGTGKTLLSQTLARILHVPFTIAD
                     ATALTEAGYVGEDVENVVLKLLQNCDYDVARAETGIIYIDEIDKISRKSENPSLTRDV
                     SGEGVQQALLKLVEGTVCNVPPKGGRKHPEQDYIRVDTTNILFIAGGAFVGLDAMIAQ
                     RTMPKRLGFGAIAPAGELEGHSEVLRCVQSEDLLKFGLIPEFVGRFPVLTTLA"
     gene            complement(28927..29676)
                     /locus_tag="PK589_10460"
     CDS             complement(28927..29676)
                     /locus_tag="PK589_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ20067.1"
                     /translation="MKSRFWSLGVVVLAGVLLQVYPALLLAADPAANAVKSPSASAEG
                     KAEETKSELYISLYMQGSRPLNRPARLQEDPFTNTDVQGGLGGGLKIGFFPAFAGRYV
                     GLEAELSGLDGKVNAPTATSGGVTRSANFRLQSINAMANLLLRYPGDVIQPYIGIGAG
                     LSGGFARDLNLQHSTIGTVHENAADGAFAYQFLGGVRANITDRLFLFTEYKYFVANYQ
                     WESEFTNGSRGPSFTLNYRTHIIAGGFGVTF"
     gene            complement(29836..30426)
                     /locus_tag="PK589_10465"
     CDS             complement(29836..30426)
                     /locus_tag="PK589_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNJ20068.1"
                     /translation="MTSRKTSCRVYSDFNCPFCYAMHERLYALGFLEQISWHGVQHAP
                     HLPQPMATWAGHLHAELKQEVKMVRRLAPELPISVPAGKPNTRLAITVAARALREDTR
                     RAGEFIRSLYRLFWVDGLDLSNERLLRLEAERQGFVGTALFGSTAPAVESMLGEWRDR
                     WEETEHQGVPLLQRADGQVLVGLVPPETLTQFLASS"
     gene            complement(30423..31250)
                     /locus_tag="PK589_10470"
     CDS             complement(30423..31250)
                     /locus_tag="PK589_10470"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNJ20069.1"
                     /translation="MVIKPRHLRWLGLSALTLCVVMHVSAVSGSHAAEIGPRVLDLTY
                     PFDETTQVWPSNPPFHRDSTQRGGTTTEAWYATGQVALSEHAGTHMDAPIHFAQGQAG
                     IDGIPVEQLMGPAVVIDVRAAAADDRDYRLSLADIHRWEAAHGTIPTGAIVMMLTGWG
                     AYWNDRQRYFGSATPEIPTTLHFPGFSKEVAEFLVRERHISGIGIDTASIDYGPSQDF
                     VVHRIVNAAGLYGLENVAGLAEVPASGARLVALPMKIAGGTGAPVRIIAILPSRDGK"
     gene            31350..32498
                     /locus_tag="PK589_10475"
     CDS             31350..32498
                     /locus_tag="PK589_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HNJ20070.1"
                     /translation="MSDQQLHFSGQRFSVEYHIQGTKEEACRRAAVLCIDQTVEAADH
                     VIPSGVIRDQLLGQMLSIDRVGDGVHRATLTFPIELLDGTMGTLQHMSFGMAGLQSRV
                     RLANLSLPDEAIAQMAGPRFGSLKLRALLEVPVRPLVCAVLKPLGLSARALASLAYEF
                     ALGEVDLIKDDQSLGDHPFCRFEERVTLCAEAIAKASRQTGRRCLYAPHVSGPWPTLL
                     DRASLAQNAGAGALLLCPGLTGFDALGTLARLPFPSLPLIAHPDFQGSHFVNSATGIA
                     PAVLFGLFPRLAGADVSIYPTFGLEYPISQEDCRGIAACCRNSLGACAPIFPTAAGRM
                     EASRIHEMVAFYGTDVVFVLGSDLRRDPTRIRSACQEFIQLLEAEVAP"
     gene            32742..34082
                     /locus_tag="PK589_10480"
     CDS             32742..34082
                     /locus_tag="PK589_10480"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNJ20071.1"
                     /translation="MALAKTSDDSRKLDKSDTLVLSPERLRVGMYVDLNCGWFRHPFP
                     RRSFKLSSASQIATIKGLGLTTVLVYPRESDPDPDAEGTLLETDPASTATVEPDTGSA
                     PAAAAADTNPPPSDEELPTHGDYHQAVELASETYRQMLGTSKDMMDALTSGSEEGMRS
                     AETMIGGISTLLTHTQAAGTVASAFDPEEIGDAGVLHSLNVATLSMMVAQQFDIDQDA
                     LQMIGLAGLLLDIGERRIPRHILDTRARRSETEHVKYQLHPYLGVEMLRGFPGIPEEV
                     LDVIRSHHERLDGSGYPEKLKGDQISFPTRIVSAVDHYDTLVNSLYAEEALSPAEALA
                     TMYKHEKQLFSTEVVVALIHTLGVYPPGTVVSLSDGSFGLVLNINFKNRMKPLVLMYH
                     PTPGQEPHTIDLLRDQTLSILRAVPREELPRPIVEFFRLKRWTGYFIQATMRTA"
     gene            34147..36339
                     /locus_tag="PK589_10485"
     CDS             34147..36339
                     /locus_tag="PK589_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ20072.1"
                     /translation="MALPRVLLIIPPLTQLNTPYPATAYLTGFLRSRGYEAEQADLGL
                     EMILRLFSRVGLQEAFHRIKASRRPWRGEVQQFLALEDSYLATIDPVIAFLQGKDPML
                     APHLARPGFLPEGPRFGADGRPGSSPFPTSPTDSARHRATLYLEDLTDLLYETIAPHL
                     FLNRYAEQIMQATSSFDGIEKALAEPLSFTDELLLEALAPLLAGSSPDVVGLTVPFPG
                     NLFGALRIAQAIKAQRPATAVVLGGGYVNTELRRLKDPRLFRYVDYVTLDDGERPFLC
                     LLDFLAGGRSQPHLCRTFVLADGQVVFRNGNTEPDLSMHELGTPTYNGLPLGRYLSIL
                     DSLNPMHRLWSEGHWNKLTVAHGCYWKQCTFCDVGLNYIARYEMTPTDVLIRQIEALI
                     RETGQRGFHFVDEAAPPAALKALALRLLETNQTISWWGNIRFEEAFSPDLARLLAASG
                     CIALTAGLEAASDRLLRQMKKGITVDQTARVAAAFRHAGILVHAYLMYGLPGESIADT
                     VDSLERVRQLFANNLLQSAFWHRFTATAHSPVGLNPAAEGITILGPTFEGFAENDLRH
                     ADPNASCPPWLGEGLRTAILNYIEQAGLTVPVQQWFPQAVQAPRVAKTWLTRALADAV
                     PEPLTAERRCVWIGGRPVVAAKGTTRVKIILPTRMEDFSISVSSDQVDWLMDLLQNAS
                     PSLHRTDRDYPRFAEARERFPHTGARGFDRFARTAAWHNARQAGLLLV"
     gene            36599..37429
                     /locus_tag="PK589_10490"
     CDS             36599..37429
                     /locus_tag="PK589_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20073.1"
                     /translation="MMTPRLLTLLMATGIVCAAPLSASAIAVYSSSGSNAASIQGSVD
                     GFRADLGTNNLANAPATGGRREINWDGVPAGFSDPNHLPGNFFNSNSPRGAVFETPGS
                     GFKVSANTGVGTPERFGSINPSFPSSFSVFSSQKLFTAIDSNIVDVKFFVPGTTQAAN
                     VRGFGSVFTDVDLLGPTTVEFFDMNNNLLSKQTVLNTPGATSLSFLGVSLEQAIINRV
                     RITAGNAALSNLDGFDFVAMDDFIYGEPQAVPAPAAVLLLASGLAALVWARRNSLLPE
                     "
     gene            complement(37608..38243)
                     /locus_tag="PK589_10495"
     CDS             complement(37608..38243)
                     /locus_tag="PK589_10495"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNJ20074.1"
                     /translation="MGLRLGDDAPNFTAETTDGTINFHEWLGNGWGILFSHPKDYTPV
                     CTTELGYMARIKGEFDKRGVKILAISVDPLDSHRGWMKDINETQNCTVTYPIIADPEK
                     KVANLYDMIHPNSLDSMTVRSVFVIGPDKKIKLMLTYPASCGRNFDELLRVVDSLQLT
                     AKYKVATPVNWKDGQDCIITPAVNDAEAKTLFPKGFKSVKPYLRLTPQPNK"
     gene            38449..38808
                     /locus_tag="PK589_10500"
     CDS             38449..38808
                     /locus_tag="PK589_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNJ20075.1"
                     /translation="MRQPATGLRIANDPMYRLLREGCIKEFNVKRAAGEPVDLTRCDL
                     RGLDLRGLEVDGLDFSECYFRQADLRGIDFRKAKLEGASINAAKISGAYFPIELTASE
                     IELSLLHGTRMRYQPKP"
     gene            complement(38823..41762)
                     /locus_tag="PK589_10505"
     CDS             complement(38823..41762)
                     /locus_tag="PK589_10505"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNJ20076.1"
                     /translation="MTFLSRYRLLLYVLSGLVALYALLGFVVAPYAVKTYGIPALSER
                     LVHPVVLGDMRLNPFTFSIELTGFEIQEPDHTPMIGFQELFVDFEATSLVRSSYLFDE
                     IRLTLPFGLVHLQADGKLNLLGLVPPGSQEAERPAPPVEQPRKEPMPSIEIRQLSIQR
                     GVIEYRDDSKKRPVTIDVVPIEISVRNFTSRGRGENAYAFTAEFGEGEVLAWEGTLHL
                     DPLESDGHVSLSHVGLKTFWPSLRDRFRFDILSGTLLVDVRYHFDTHLAPINLQVTEG
                     KVQLADFRLSEAGSRDPVVTVPSFGIEGINMDLSKRELGIGTVALTGAEVRAWLGQDK
                     VVNFQPLFAPVAATADASPPSEPPAPDAGQAWKVAVQSVEVANTQVAFEDRSLATPVE
                     LLLDDVHLTAGDVHVPFKGAVPVTAGFRLNQQGALEAKGTLRLDSLQAALALKLDHIG
                     LRPFQPYLDRFVQLEVRDGELMLDGDLVYRSRHESEPMLQYRGQAGLNNLHVADRAST
                     KEFLGWTALSLSKMALDVAPTTVKIGEIALRDPAFRLITDKDGSTNLSRVLRPSDTPP
                     APASKPVEPEAPKKPATPTPVEIAVVRLTNLSATFSDESIVPAVSTGITDLTGTIKGL
                     SSRQIAKAEVALTGKVDEVAPLKIQGQINPLSEDTFTHLTFVFQGVDLTSVSPYAGKF
                     AGYPITKGKLSLDLMYQISKKQLVGENKVLVDQLTFGDKTDSPDATTLPVRLAVGLLK
                     DRRGRIDIDMPVRGDLNEPDFRYGRVVLNALVNLLTKVVTSPFSALGGLVGGGGEDLQ
                     FVEFQAGSDSLAEPEQRKLESIAKALEERPALRVDVTGVADPVRDRQALALQKIADEV
                     LRRFTKGGTKNLQAVPSPSREFELLSDLYAEKLGKQPMKREELPGGKSVERVLTADEL
                     RQQLFPAMTVEDAELRGLAQERAKAIREHLIAQGHLPDERVFLVEAELAASDKPQIRV
                     HLSLTGS"
     gene            42066..42473
                     /locus_tag="PK589_10510"
     CDS             42066..42473
                     /locus_tag="PK589_10510"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNJ20077.1"
                     /translation="MRFLVLMIPGVYQPNTNRQPKANFTPTAEMMAKMGRFNEELKQA
                     GALLSLDGLQPLATGARLAFSNGKASVTDGPAVDAKEVVGGFWMLQATSKQQVIDWMT
                     RCPALDGDVIEIRQVFELSDFQEDVQATAKKHG"
     gene            42566..>43038
                     /locus_tag="PK589_10515"
     CDS             42566..>43038
                     /locus_tag="PK589_10515"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ20078.1"
                     /translation="MTKPAKNTICLWYDRDAEEAARFYAKTFPDSFVGAVHRAPGDYP
                     SGKKGDVLTVEFTVMGIPCLGLNGGPAFKHNEAFSFQVATTDQAETDRYWQAIINNGG
                     EESECGWCKDKWGLSWQITPVVLTDAVTNSDPAVAKRAFDAMMQMGKIDVATIEAA"
BASE COUNT         8807 a        13164 c        11986 g         8681 t
ORIGIN      
        1 tggttccacc aatagaacaa gggaaattgc tcggtatagt agaccgggtt gccgtaattg
       61 cttctcgtat actcctgcac cagccgtccc gtcacatagt caaccttgcc gtcaccatgc
      121 aaggaataga gcatgatgaa catcctcgac tcgtgatcgt agagctcatc gagacggctg
      181 ttcagatccg gctcgatcgg gaggggatcc atggaccaac cgggagcagt tggaagcaac
      241 acggagagaa acaggaggat actcacggct ctgcggaggt tcgtcatagt acagaaatcc
      301 gactgacacc acagccaaca caacgtgcgg tcatgctacc acccgtatcc agaccgttca
      361 agcgctggcc cgactttttc gcgccacggt caccttgctg atctgatgcg cctcctctta
      421 taatcgcgca cgatgtcttc caccatgcct cgcgcatcac tccatacatt gggctgccgg
      481 ctcagccaat cggagaccgc catgttaggc aatagcctga cgcgccaggg ctaccggttg
      541 gtggagtatg gcgaggaaac cgatctgctc gttctcaata cctgctcggt gacggaaaac
      601 gccgaaaaag actgccgcta cgcggtccgc aaaacgttgc gtcattcgcc ccatgccttc
      661 gtggctgtga ccggttgtta tgcccagacg ggcgccgcgc gattgcagac cgttcccggc
      721 atcgacctga ttgtcggcac ccagtacaag atgaatctgc cggattatct tccggctccg
      781 acgcagttgc gaaaacaacc ggaacccgag ctccgtcata gccggacgat cgatcgcgag
      841 gacttcgtcc tgccgggtac cgcctattcc gattccaccc gggcgctgct caaggttcag
      901 gacggctgcg acttcatgtg cagcttttgt ctgattccgt ttgcgcgcgg gcgggagcgc
      961 agccgggtcg ccgacgatgt cttgcgggaa gcgcgtgagc ttgccatcca cggctatcgc
     1021 gaactggtgc tgaccggtgt gaacatcggc caatattcct atcaagggat ggaactggtc
     1081 gatctcctgc aagaactcga agccattccg gaagtcgtcc ggatcaggat ttcgtccatc
     1141 gaaccgacca cgttgcctac ccggctgctg caacacatgg cctcctcgac gaagctctgc
     1201 cactatctgc accttcctct ccagagcggc gacgacggga ttttacaggc catgaaccgg
     1261 cgctacggca tacgtgagta cgaggaattg gtcgagcagg ctcaagccct gatgccggat
     1321 ctgggtttgg gtaccgacct aatggtcggg tttccgggtg aggatgaaca ggcgtttgcg
     1381 aacaccatgg ccatggccgc acggctgccc ttctcctact tccatgtgtt cagttattca
     1441 tcccgtcccg gcacgccggc ggcccgcctg gaacctcaga cgccgggcac catcatccgg
     1501 caacggagta aaaccctcgc agagttgtct cgcaccaagg cgctggcgtt ttatcagcgg
     1561 cagatcggcc gtaccgtctc cgtgttgttc gaacaggggg agcacgacgg ctttcgaacc
     1621 ggcaccacgg cgaacttcac tcgagtggcc gtcccggccg atgcggtcgc ggccggctct
     1681 atccaccctg tcaccattac cggcatcatg gatggcttgg cctatggtca tctggcctct
     1741 ccgtccgtgg agccgacata tagaccattg ttatgacaca acccaacaag ccccataccg
     1801 tccatatcga aaccttcggc tgccagatga acgagtacga ctctgaactc gttcggacgc
     1861 tgcttcgtaa agcgggattt gaattcaccg aggatcgcga gcgcgccgat gtgatgctca
     1921 tgaacacctg cgccattcgt gaaaacgccc acaataaggt ctatgggcat cttgcggaac
     1981 tcaaggccgt gaaggaacaa cgcccgctgg tcgtgggcgt gctgggctgt atggcgcaaa
     2041 acctcaaaga agaactcacg gagaaacagc cgctcgtcga cgtgctggtt ggtccggacg
     2101 gataccggca gttgccgggc ctcctgacca acgcgttgaa tgccgaagag caaggactct
     2161 cgcgccgtgg gctggccgtc gatctgtctg aatacgaaac ctacgatgac atccttcccg
     2221 agcgcgacgg cggctgcaat gcctggatcg ccatcatgcg gggctgcgac aatttctgca
     2281 gtttctgcgt cgtgccctac acgcgcggac gcgagcgttc gcgcgatccg cagggtatcc
     2341 tgcgcgaagt ggaggtctcg gtggaggccg gccacaccca gattacgctg ctcggccaga
     2401 acgtgaactc ctaccggtat gacgattggg acttcgcccg cctgattctc gcggtggccg
     2461 aagtaccggg tgtccagcga gtccggttta cctcccccca tcccaaagat tttcctgccg
     2521 cgctcttgga tgctgtggcc ggtcatccga acatctgcaa gcacattcat ctgccgttac
     2581 agtccggcaa cgaccgtatt ctcgaactga tgaaccggac ctacagccgg aaggaatatc
     2641 tcgacctggc caccacaatc cgccgtcggc accccggtat cgccctgacg acggatatta
     2701 tctgcggctt ctgctcggaa accgaagagg agttcctgga tacgtttcga gtcgtggagg
     2761 acgtgcaata tcactccgcc tatgtcttca aatattcgga gcgaaaaaat accattgcgg
     2821 cgcggaaatt cccggacgat ataccggaag ccgtcaaagg tgaacgagtc agccgcctgg
     2881 tagaccttca acgtcccgtg accgcccgca tcaaccggga gttgatcggc cagaccctct
     2941 ccgtgatggt ggaaggcgac tcaaaaaggt cggcggatca atggatgggc cgcaccgata
     3001 ccggcattta cgtcatttgg aacaagagcg acgctccggc cttgcttgga agccttcaac
     3061 cagtcaccat tctcgatgga agcgccgccg tcctcatggg ccggggcgag cctggtgcca
     3121 ctctctcgtg attcatccct cagttgttca caatcgaatg acccatcacc ggtcattgca
     3181 ccggacgatc ggctcccctc gcaatgtgga ccaatcgtcc tgtgcagaaa gaggaggatc
     3241 ttattgccca gttctgaaca attcttgcca aattttagac gcccgcgcgc gtcagactga
     3301 caaaaataag gaacgcagat acgacgtagc gagttcgtat tttccaacct tctcccgagc
     3361 ccatcacttc ttacctctca acggagcctt ttcactgcct tgaaacatca atagcttagg
     3421 ccccagtgtt gagcgtgtgc cgccacgtct cagctcaaca tccattacag acagcaattg
     3481 aacgggagtg agaaatcgca cttctgaagt ctttgtaaac agggcatagc ctttgcttgg
     3541 tccagtctgg caaccacacc aaccacccga ccgagccaga ggacgccgta tcatgaagaa
     3601 agacatcatc cagacccacg tagaagaagc ccctaagggg atcagcctgg aaaccatcat
     3661 cgcggttggc gtcttcgggt ggatggctct cggcgtggtg atgatgggtc tggggatctg
     3721 gtaatcccga tccgtcgttt cgcctgttct ctctgaaacc gcaccgcctc tgttcgtcac
     3781 cgcttcgacc agccgagaag gaagaccgcc atgctgattc acggctccca ctcctttttg
     3841 atgcaccgcc tgaactccgg aatcctcgga ttgaccttga tgggagccct ggcctccggt
     3901 tgttcctcca ccgccgcctc ggttcagcaa ccagccgcca tgcagcctca aggctccgtc
     3961 cggctggaag aagtcgccga ttgggaattt gaagcctccc atcccagctc catagatgcc
     4021 gccacactcg acagcctgtt gcgggccgtc gtgattgcgg aggcgcagac tgatatgtcg
     4081 aatctgcccg tggatggcag caagccgatg acagtgttca gcgatgagga tgtgcgttat
     4141 cttgctcccc tgctagccca agcgttgtcc caagcgcaac ccgaatacgt ggtcgctttc
     4201 cggctctcct catcggcagg gtcaggctct gaacccaccg cgggcaccct ttacgtgaag
     4261 aacgaccggt tgtatctcac cctttccgag caccacggtt cgctggccaa aaccgagtca
     4321 acatggctgg ggagcgggcg agcggctcga atcgtgagcg tcatgccggc ctccgccggg
     4381 cagctcgaac aagcgttgcc ggccgtgata caaggacggc agcagatgaa gtcgcttgcg
     4441 atcgactatc accagtttgc caaaaagccc gaaccggctc ccatgaccgc ggcgaccact
     4501 gctccggctc cccaacctgt ccaagccctc agcgtgcgaa acgtccctgc cgtggcaccc
     4561 gatcaaccgg ccaagcagca atccgcgccg gcgccagtca tggacaaggt ggcgtcgaac
     4621 caggcccctg ccgaagatga acgtttgaac gagacgcttc aagaattgca ggacacgaag
     4681 gacgccatgg cccgtaagga cgccaagatc agcgcgctgc gaagagattt agagtcgatg
     4741 cggatgcagt tggaaacgaa ggataaggaa ctccgggcag tgaaaaacac tcagcagcag
     4801 caacccaaac gcgagacccg caataaggca gaactgacgg tccgctgacc tcgcggaccg
     4861 gattcagccc acatcgacca gctccggctt cagcagcaca atctccctcg atatcggcgg
     4921 ccggaaattc atccggcagt agcatccata ggtcacatag gtatcctgca ggcaatagag
     4981 ggcaatctct tttccctgcc cgtccgccca catttgcgcg acctgggaac cactccccga
     5041 tttgctttct acattcaacg cccgggcaag cacatccagc ttgacccatc cccggttatc
     5101 ccagttgctc caaatggcca tggtgtcgta gaccggctcc gcgcggaact tggccaggtt
     5161 gatctccata ctgggcttca cttgttgaat gatcgaccgt tttttgatga agggcagatc
     5221 gaaattgagt ccgttgtggg tgatgaacaa cgacggacgc gactggccca gatggctcca
     5281 gaaggcccgc aggagtgcct gctcattccc cccataccac gagatggcac ccctcggttc
     5341 cagggtatcc gtgaactcca acagaccgat gcagacgatc ttgctgaacg tgccgtcgaa
     5401 ggaagacttc tcatacagtt catcatcgag ccgctgctga gcctccgcct cccccacggc
     5461 aaatagatca ccgccgctct cagcgaaagt gacctcgccc gacgtgagct gccgccctgc
     5521 gatccgcgcc cattcttctc tgggagcctg cacggtttcg atatccagca ccactttcat
     5581 acgcccctac accttcctct gctgaagcgc cgcaatgacg ggctggtcgg ccggcggaaa
     5641 ctcgaaggag gccatctcat gaggatagac ccatcgaagc tccgcgcaat cgatcgcgat
     5701 cgcaacaccc gcctcgatgc ggcaacgaaa gaaatgcaat tccacaacct tctcgggata
     5761 ttcatgccgt acgatctgaa agggagtcgg tgcgctgact cgaacctgca actcctccca
     5821 taactcccga tgcagacatt cctccagcgt ttcgcctgtt tcacgtttgc ccccgggaaa
     5881 ctcccacaac cctccaagat gcgcgccggc tttgcggcgg gcgatcaggt agcgtccttc
     5941 gtgagcaatg attccggccg ctacttcaat cacctgcatc gcgaccttcc ccgtatcgag
     6001 tcgttgcacc atcatccctt cgcactctgc gatcagtcat gggatgaccc gttcgtcgag
     6061 gattatttct caccgaacgg ataggttttg cacagcggtt tcatgggaca gaggaggcaa
     6121 tagggattac gcgccgtaca aatggtggcg ccgaaatcca tgatcgcctg gttgaaatcg
     6181 taccccttac ctcgaggaat caacgcttcg gagagttccc agagcaacgc tttctgtccc
     6241 ttcggatccc cctcggcgat gaacacccgg tgcaagacac ggatcacgtt cgtgtcgagg
     6301 atcggcgcgt cctcgttgaa cgcgaaggag cgaatcgcgc cggccgtata ccgcccgatg
     6361 cctttgaagg acaacaattc atccgcatca ctcggtagtt gcccgccata gcgggccacg
     6421 gtttcacgcg cgatactgtg taaccgctcg ggacggatgt tgtatcccaa cgggtaccag
     6481 gtctgtttga cctccgctac cggggcatcg gccaattcgg caaaggacgg ataccgctcc
     6541 aaaaattcat ggtacttcgg gatgacccga tccacttgcg tctgctggag cattacctcc
     6601 gagaccagaa tatgataggg atcggacgtc ttccgccagg gcagatcgcg gccatactct
     6661 ttgtaccact tgagcaaccg gttctgaaac cgctgcttct gtccgcgcgc aagcgggagg
     6721 gcgggagttg acgtctgctt cttccgtcgg gaaggtttct gagtgcgagg ctttgtaggc
     6781 ataacggtcc gtggaggcgt ggcggcattc taaaaggcgg tggggtgaaa atcaaaccag
     6841 cgtgaaggaa cgggagagga ccgcaggaca gggtctccca atggaatact ccccgtcctg
     6901 cggcgtgtga tgcgacgtta cacaagcgat ggtgttactg cggtgaggcc tgcaccttcg
     6961 ttccctcttt tacgggcgga tcgatcacaa tattgggtcc ctgcaccttc ggcaaaatcc
     7021 cggctccggg cttctcgagc aggcgctggt tttggtcgtt gatcgaaagt ttctgtgaca
     7081 cgtgccggtc atcaaatgcg gcggagccag caagtgcgga ttccccgccg ccgttggcac
     7141 gtccaggatc gttagcgaga gcctggccgt tgaccggatc ggtcgctttg cccatcggat
     7201 acccgggatg ctggggcaac agcgcaggat tggcaaaggc tacggcagct cccaacattc
     7261 caatagccgc caacgcatat atggagaacg aggctttcat acgacacctc ctttggggcg
     7321 gataacatga cagcgccctg cggccgacca gccgcagggc gcacaatgaa cctccggagt
     7381 tgaaaggagt cctctcttgc tcttgcggaa caattcaccc cggcatggca agtatctccc
     7441 gatacctttc cagcttcttc ctccataaga acctagtcaa gagggcgatc atcatccgac
     7501 taagccactc ttccgggcat gattgatcag cacgtagttt gaagaaccac aaacggatgc
     7561 cgattttcag gtcccattcc tcaaatcaag gaccagagcc gatttcctgc cctaggactt
     7621 tccccaccgc caaatgaccc gagacattat cctgatcgca caggcaatgg agggcatggt
     7681 atgtcagtct gccggcccac tcgtcgcccg aggccttcgc tctttcgcat cggcgatgaa
     7741 gcgcgagacg ttgatgcgac gagcggctct gtggactcgt ttcaggcttc acctggagtt
     7801 cgacggtgca ccagggtagc gccattattc actacacccg aggatgcaca gcaatacatg
     7861 tccatgaccg ttcattaccg tgtgtccctg tccgcggatt ccacgacgac tggagaagga
     7921 cgcctgctag atctctccct ggaaggctgc cggatcgaat gcgaccggca tttgccggtc
     7981 aatacctacc tctcgttacg tctgcaaatc tcaccgaatg agatgccgat tctggtggac
     8041 cttgccgcgg tgcgatggac tcgcggtacc cattgcgggg tgcatttctt gtccgtccag
     8101 ccgctgcaat ccgcacggct gcagaccttt ctcgccgccg cgtcgccccg gaaagaacca
     8161 ccgactcccg gggcaggtta atccgatctg ctccagccac tagcccgaat ttgcccttgc
     8221 cttttgctga tcgctccagc atcctagggg cccacctagc gataacccta ggtgggctgt
     8281 aggcctatcc actaccagtg aggaatgacg gcatgaacga ggatcatctg agccggagaa
     8341 tcgatgtgct ggcaatcggc ctatttggcc tggcggtggg cgcactgacc ctgggagtcg
     8401 cccaacttgg ctggattcaa cagaagaaca tggtgggggc cctggtcatc gcgctgatct
     8461 tcggcggagt ggtgcagctg ctggccggca tcaccgacat tcgttataac gagcagttgg
     8521 gcggcacggc gctgacgatg tatggattcc tctggattac cctctgcacc gtcaaactgg
     8581 tgagcgccag ttccaccgtc cagtttgatg cagtgctcta cgcgccgatc aatctggtct
     8641 acgcggtctt ctccggcgtc atgatctttc tgacggccta tcgcaacctg acgctcagtg
     8701 cgctgcacgt ggtcatcacc ctgacgtttc tggcgaccac ctttgcccgt ttggacttca
     8761 tcagtgagtt gctgccggga tggggccata tcatcgtcgg acttatggcc ttttatcatg
     8821 cggtcggcag cctgacgcag gccttcacgg gaaaatccat ccttccactc ggcccgccgc
     8881 tcctgtttca cacccccaag cacgtgcact cgatcgcgaa agtggtgtaa ctggtaggct
     8941 gctgaaaagg gctgtccagc aaggccgcag ggagtgaaga ggcgaatcgt actctcctcg
     9001 tacggtgagc ctctgatcga agcgagaacg ccgctggaag ccttttccaa cagcctacta
     9061 ccagtcggca cctaggcgat ccacaacgag ttggtgcacc accgcctgag ggcccaaaga
     9121 ggcgagatac agagcggcct ctgcgatgtc gaacgggtcg attttttcgc tggccgctct
     9181 cgcttctggc gtggcatcaa ccagttcgtc ggccaccccg ccgggacaga gcgcgctcac
     9241 cttaatccga taggctcgcc cttcgtccgc caacgacttc gtcagcgcca tgatggcatg
     9301 tttggaggcg ctgtaggtgc ccgtcccgct ccaggcctgc acacccgcca cgctcgacat
     9361 attgatgatt gtcccgccgc cctgtttttt cataagcccg aaggcggcgc ggcagcagaa
     9421 gaacgttccc cggacattgg ttcgcatcac ttcatcgaac gcctcggtgc tcgtctctgc
     9481 cagacggcgg ccgccgaaga tgccggcatt gttgatcagg atatccagac gctggtatcg
     9541 gcggatcgtt tccgccatca gctgctccac ctgcgattca tcggcaatat ccgtctgtaa
     9601 tgccactgcc cggcccccgg ttgattcgat ctgcgcgacg gtctcttcgc acttgcagaa
     9661 gcgccgtgcc gcaacgacca ccgtcgcccc ttcctgcgca aaccgcaagg caatcgcttt
     9721 gccgattccg ctgctgctgc ctgtcaccac cgaaactttt ccatccaagc gcttcatatc
     9781 gggcctcccc ttaccttcga tgctcaggac aatgtgtgac gtcctattcg tccataggat
     9841 acaggtgaaa cccggcatgg agaaagaagg tggaagcaac gctaccggaa gcggttggca
     9901 gaaccactac tgaggatttt tttcggttgg attttttacg ggggaggtgt gactgtccac
     9961 attgtgtccg agcatgtggc ctacccgtcc aagcacgatt tgctccgcnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnntc gcaaggcgag atacaattga tccgccgtgc
    10141 cctcgctcat ttcttcaacc gcgaccgatg tgccgccttg gcgcaatgcg cataaccgcg
    10201 gctgccctcc ggtgtcgtca ctgatcaatc gttcataatc tccgtcggtc atgagcgcga
    10261 aataagctga ggctttcgta atcatgggcg cctgtgcccg ctcccggaag cgattcaagg
    10321 cttcctgcag cagtgcgcgg gccagtttca gtctggccca tggcctgatg gctgagcgat
    10381 accgtgcggc agccgactcc atcgcttcac gggcacgggc tgcgcggtct gaggagtcga
    10441 tggcgttcag agctaatcgc gcctgctctt ccgcgcgacg ggcagccgcc tgctcttctt
    10501 cacatctcgt aatttccgat cggcactgtt cccgctcggt ctcgatcgtc accgcatccc
    10561 ggccggacag gcgctggcgt agctcctcga cggaaaggga cgatgcggca gcgatctgtt
    10621 gccgcacatg tgtatggctg gcggatgcct cccgtttgcg tgaggccgcc tcctcacgtt
    10681 gcggcaagtc ctcgagaccg gtcacccccg ccactgcgca cagccgtgcc aatatggtct
    10741 gctgcgcggc ccggtccgag gcagcgcctc gccgcttctc ctcggctttc cgctgatccc
    10801 gcagcagcgt cgcctgctcc tgttccgcag cctttgccgc cgcgagcctg ccgcgtaacc
    10861 gatccgcaaa gtcctccacc atctgaggcg ccggtatatc gagtactgcc gccacatcct
    10921 tggactgtgc ggccaggtca tcgagaatag cctgcacctg ggctctgcgc tgctttgcct
    10981 cacctaaggc tgccgcctgc cgtgccgcgc catccagttc gtcgagacgc actttaatcg
    11041 catccggaat cgtatcggag gcgaggaaca accgcgcatg ccacgcctgc aatgtggacc
    11101 ggtggcgccg taactccgcc tccatctcag ccatcgaacg gtcgagttgc tggcgttcga
    11161 tccgttgcac tgtggccgct tgttctttcg cgttgtgagc ggcttcagcc tcgacggcct
    11221 tgctgtcata ggccaccgcc tgctcgatca acgacggcaa cagcgtcaca tccacggggt
    11281 tcgcctcact cactggatgg cccagcttta gcaaggctgc cctgactcca gccaccgcat
    11341 tgttcgtctc ttcctgcaac cggtcacttg cagcgcgcag ccctgccacg cgttctgcaa
    11401 tctggagcgc gtcggctcgc cgtccctgcc actcccgtaa tggttcggga tccaaatcag
    11461 gcagaccggc gtctttcagc cgttgtgccc atgcgtcaag ggtcgccccg cggcctgccg
    11521 aaagtcgtgc tatctgtcgt tcgatatccg atcgccggcc ctccatctgc tcgatgcgtc
    11581 ccgtgcattc ctccaggccg gcggcgcgtt tggcatccgc gcgcaacaga tccgcctgcc
    11641 ggtcggcctc gctcagagcc gcttcgaaca tctccggtgt ggcccgtgca gtatcaaacc
    11701 ccaaggccag ttgatcgagg gtctgagtac ggtcgatgta cgcctcacgg atggccttcc
    11761 acctgtcgtc tcgcttggcc cgcgcctgac gtaacgtctc cgccgtcacg atttcgccct
    11821 cggcggccaa ctgcctgagc cgcagacgtt gtccatccag atcgcggccc actaattcct
    11881 gttcatcgcg caacttgcgg atctgctcgt cgagatcgtt caattcctgc ctcgtacgcg
    11941 caatctgtgc gtcgagcaat ggttgagcgc ggcgcaacgc ctggtcggag gccagtccga
    12001 catcggccag agcctgttcg agcagaaggt ccatttcacg ccgctgctga tccagttcct
    12061 tctgccgccg gaccacatca ccaagcgatt gggcatggcg aacggctgtc atgagttgct
    12121 gtctggagcc gggatcgatc gggagccagg attcctggtg agacgaagcc agggcctggt
    12181 ccaatgccgt ggcgcgttgc cggatatgtt cgagccggtc ctccaatcga ctcacctgca
    12241 tcaggtgatc gtccaggacc aggcggtcgg cggcagacgg cacggcgtgc agcacatcac
    12301 gcacctcacg tccgggcgcg agtcgcgcga tggctaatcc gaggtccgca tcgagtgcgg
    12361 caatgactgc atcctgttgt tgcacctcga tccgatagcg agccgccgcc gcgaccccag
    12421 acacgaatcg ttcgatcgca gcggcatgct ccaggaccaa tggctcgagg gtcaacgcgc
    12481 caagcgcacg ggcgcatcgc tccgcctcga cctcagcttc tctcaggctg tgctcagcgt
    12541 ggttgagttc ctgtaacgca gccaaccgcg cttcccgcgc ctgttcgggc aagtccggaa
    12601 ctccggcgaa cgacgccaac aaggccgtcg ctgcgtcatg ggtgcgcaac aacggtccga
    12661 ctgtgcgcaa ttcgatcaat tcgttctcac gccggcgcag ccgttcgaga gtcatactca
    12721 cgtcagagag cgtcgctgct gccgcctcgt gcgcgcgata caagctttgc cactccgccg
    12781 gcttggtttg ggcttcgcgc caggcctgcc gttgttcatc gagccgccgg cgtgcctcat
    12841 tgatcaccgc gttctgcgcc cgtccatgcg ggctatatag cttcttcgcg tcggtctcca
    12901 acgctgcgag cagcgtcgcg atgccgctgg tccctgcgct tgcttcgaac aacgctgatc
    12961 ccaaatcccc ctcgccgcgc agaagcacct gtccgccttc gcgcaaacgc gcatgggtga
    13021 gaccgaacat ggcctcgaac tccgcccgct ccagcccgcc ggttaacccc cgttcgtggc
    13081 ccggctccat gacgagcgat tgatctggtt gctccgtcag aacattgagc cgcgccagtg
    13141 tcgtcccctt gcccttgcgc cgcacaaaac cgatcgcctc gccattgatc gcggcgaaaa
    13201 ttcctccgat ccgaaggtca ccggcggcat ggaggaaatc gtccgcgctt cgcacaggga
    13261 tgccgaatcg caaatccatc atggctcgca aggccgacga cttgcccgct tcattgggac
    13321 cataaatcac gtggaggttg gcagggcccg tggaaaaatc cagcaccttg ccggtgaatg
    13381 gaccgtaggc ctggagcagc agcttgagca gtttcatgcc tctgctccaa gatcagtcag
    13441 ccgtgccaat actgtggctt ccgcctccat caggagctgt gtcaattcga cgcggtcgtc
    13501 gagcttcggc acgtctccag ccgccacttc ctgcggaagc gagcccagca gatcgccgag
    13561 ttcgacctgc gcccagcgct gcaactccga ttcgtcaccc gccacggaat cgacgagacg
    13621 gacgagttca cccaccgcat cctggcgttg cgccgtttga acgcgatcaa gcggagtagt
    13681 gaggtcaagc cggacctgct caatccagac ttcggcttcg ccaatgtcct gtgccgcccc
    13741 atacatcgcc gcagccaacg tgccgggctg gctcgcttcc aggcgatgga gttccgtgga
    13801 tcccgtcagg gttacccgta ccgcgtgcag gcgatccatt gcgccgatga gcaccggctc
    13861 cagggcatga cggaaggcct ctcccaaagc ctccagcctc tgtacgccat cgagcgaaac
    13921 gatcagctga ttccagcgca ccacatccag cgccacgaat tcgctttcga ttcggcccgc
    13981 ctccaccgtc accaactcgc accccttcgg ccccgtttcc ttcgcatggc ggccctgcag
    14041 gttgccggga aatacgatgt gcggccgctc ggcgatcact tctcgcgcat gcacatgccc
    14101 cagggcccaa tagtcgtatc ctttgtggtt caacgtcggc aggtccgtcg gcgcgtaggt
    14161 gtcatgcccg gcgcggcctg tcaggctggt gtgcaagaga ccgatattga agaagcccgg
    14221 cacaggcgga ggataagaag ggacaaggtc ttcatcgacc gcccgttcgg gaaagctgcg
    14281 gccatggatc gccaccgagc gatcgtcgag cctgatggtg tgagccgcgc gactggaaaa
    14341 tacggtgaca ttcggcggga ggacgagctg tttggagatg acaccctgcg catcatggtt
    14401 cccttgaacg atgaagaccc ggatcccctc tcgctccaac cgaaccatct gcccgcgaaa
    14461 aaacaggccg gtatgaaaat cctgccagtc gcagtcgtag atatccccgg ccaagagcaa
    14521 aaagtccacg tgttcactga tcgctgtatc gatcagccgc tcgaaggcac tgcgtgtcgc
    14581 cgtccgcaac cgctcaaccg gagcgccgtc gtagcggtcc agcccgcgca ggggactgtc
    14641 aatatgaagg tcggccgcat gaataaagcg catgacgtac aggtccagag gagttcacga
    14701 tcacccacgc atcctacgat cataaatccg gatggacagg acaagcgcgg tgcgatcccg
    14761 catgatacgc gagtggccag gtcctcacaa tgccggcgcg tgactcctct gtcatctgag
    14821 tcccgcccgc cggactcggc gggcgggaca aagccagcct atcgtctggc tgcttgtttg
    14881 cggcacccac attgacagtt gatctgcacg caacggcagg cattgggctg acacccgcat
    14941 gcacaggatg aacagtcgca tgttccgcag cacccgctct tggcgatttt ctgggaggtg
    15001 gatgtcttcg catcggccat ggtggtctcc tttggtaggt caacgcgtga ttgcgtcgtc
    15061 acacaggctg acggagagcg ttcgaaaacc ttacagttca acagagagtt tggtaagctg
    15121 ccgtcatgtc actcgaatcc acaccatcga cagccctttc gccggaggcc attgcgcagc
    15181 tggtgcaagg acataagcag ttcctggcgt tcctcgagcg gcgagtcgaa tccagagccg
    15241 tggcggaaga cattctccaa agcgcgtttg cacgtgggtt agaacgcggc gccggcgtgg
    15301 acgaagaaaa ggtcgtcgcc tggttctatc gtgtgctccg caacgccgtg atcgatcact
    15361 accgccaacg atctagcgct gagcgcgcgg tggaagcctg gaccagagaa tttcccgatg
    15421 tccaggaacc ggcggcggaa ctgcgacagg agatctgcca gtgcgtatcg gggttgctcg
    15481 agacgctcaa accggaatat cgcgaagccc tgcaaatcgt ggatctgcaa gaaggtaaat
    15541 tgaaagacct ggctcaacaa tcagggatca cagctgagaa tgcggccgtc cgcgtgcacc
    15601 gggccagagc ggccctgcgt cgccagatcg aaaaggcctg tggcacctgt tccgtgcatg
    15661 gctgcctcga ctgttcctgc gaggcatccg gcggatcgac ttgcggggca tgaggacaat
    15721 gcagcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatgg ctcagcggtt
    15841 gttcgacaaa acgaagtggc tggcctccaa cgtggagaat ttgcggcacg agcccgtggc
    15901 ggcggatttg cccggcatcc ataaatatgc cgtcggcgac tggcgcatct tttactcgat
    15961 cgaccgatcg gaacagatgg tggacattca ctacatccaa caccggccgc aagcgggccg
    16021 cactcccgga gcctgacgat gctcaccctt acgcagaaag ccgtcgaccg gctgcgggcg
    16081 ttgttgctgg agcatcctga agacccggtg gtgcggattg ccgtgcgcga tgtgaatgag
    16141 cagcggctct cccttgccat tacactggaa gcggcggttc gcgaagagga tgtggtcgaa
    16201 cagattgaag gcctgacgat cgcattcgat cggtcgagcg cacatcggac aggcgggatg
    16261 acggtggatt accaggccgg acgtttcagc tttgcccatc cccctgcgaa tgagctgggc
    16321 ctcatcatgc ccggccgcaa ctgaagtgag tgaaccgtta caggtacaac gtcagtgtgt
    16381 ttgacgtgtt ccgtttcatg ccttggcgcc gctcacgatt tcatccgcgg aaatacgctg
    16441 ccgaaatgca tagtgaaggt acctcgcgcc acatccgcaa catagcgttc cagggcgtcc
    16501 tggatcacgg gaaaggccag gtcgtcccac ggaatggcat ccagcgtgaa caattgcaca
    16561 tccaggcttt ccgtaccggg tttgaattcc ggcgtgcgca tggtcccgcg aaaaatcata
    16621 tgcacctgac tgatgcgggg caggctcagc acggcataca gggaggtgat ttccacttcc
    16681 gcatgcgctt cctcgaacgt ttcccgaacg gcggcctgtt ccgtgctttc gccgatttcc
    16741 atgaagccgg caggaaacgt ccagtggccg atgcgaggtt cgatcgcccg gcggcacaac
    16801 agaattttgt cttcccactc gggaatacaa ccggcgacga ttttcggatt gatgtaatgg
    16861 acgacctgac aggtttcgca gacaaaccgc ggcaggttgt cgccgggagg aatttttttc
    16921 gacagcccgg ccccacactc gctgcagaat ttcattgcgg ctcccgatca cgacgagaga
    16981 aagacagaaa tggatagcac aattcaggca ttctttgcac cctgcccgcg cggcctcgaa
    17041 gccgtcctgg cggaagaact gaccatgctg ggcgccaccg ccgtcaaggc caccgccggc
    17101 ggagtagcct ttcgcggcac gctcgccctc tgttatcggg taaatctcca aagccgcatc
    17161 gccagccgca ttctgctgcg cattgcggaa ggggcctatc gcgacgaaca cgatgtctat
    17221 gacgcagcct acgccatccg ctggcaggat tggtttaccc ctcagcaacg catcaaggtg
    17281 aaggtgagtg cccaccgttg cccgctgaag agcctggatt ttgtgacgct ccgggtcaag
    17341 gatgcggtgt gcgatcgatt ccaatatgcc aggggcaaac gcccgtccgt cgatacccat
    17401 gcgccggaca tcctgattgc cgtctactta gacggaaccc aatgcacctt ctatctggac
    17461 acctccggtg atccgctgtt caagcgcgga tggcgaaagt cctccggcga agcgccgatc
    17521 agggaaaatc tcgcggccgg aatcctgcgt ttgtccaaat ggacgccgaa cgtgttgctg
    17581 tacgacccca tgtgcggcag cgggacgttt gtcgtcgagg cggccttgat ggcgggtcgc
    17641 atcgcacccg gcatcggccg cggctttgca tttgaaaagc tgctctcttt tgacgccccg
    17701 gtgttgaatg acctgcgggc ggaacttcag gccgcggaga ttcccgtcaa gtccgggttg
    17761 attcacgccg ccgaccagag cgcgcaagcg atcacctcga tcagagccaa tctcgcgctc
    17821 gccgggctca cggaggcggt gacgctgcaa cagggagatg tcctgcagct tcccgcaccg
    17881 gcggaggccg gcctgctcgt caccaatcct ccgtacggcc accgcatggg agacgatgag
    17941 accttgaaga cattttatcc tcgattcggc gaccatttga aaaaacactt ctgcggttgg
    18001 accgttcaga tttttacggc cgacatgaag ctgcccggcc ttttgcgtct ggcgccttca
    18061 cgcagggtcc cgttgttcaa tggcgccatc gaatgccgcc tgtttgaatt ccgcatggtc
    18121 gccggcagtc tcaggaaacc caaaccacca gcacaatcct ccccgggcca cggccaccac
    18181 tgaccggttc tccgggcagc cgaatacatg cttgacagtg gagcataagc ctcctatttt
    18241 caatctatgt cattcgacgc gccatcatat ctgtgtcaca caccatgcgg aactctatga
    18301 gcgcagccca cgcgcccctt gaggctccgc agcaggagca cgacctgtca ctctcgcgct
    18361 tgatcgtggt ttcgaaccgc gagccctacg aacaccggtt ggtgaaggat catctgattt
    18421 gggaacgcac gtcgggggga ctcatttcgg cgctcgaccc gatgatgcga cgactgggcg
    18481 gaacgtggat tgcctgggga agcggcaaag ccgatcggga tgtggtggac gaggatatgg
    18541 tggtcgaagt tcctcccgac gctccgacct atcggttacg ccgcgtctgg ctggaaaacg
    18601 gagaggtgaa gggcggctat caggggtacg cgaatcaggt cctctggcca ctgtgtcacc
    18661 tcaccctgga tcgcgttgct taccggaaag cattctggca tgcctatcag gccatgaatg
    18721 cacgctttgc ggacgtggtc ctcggtgaac tcgccgaaaa acccggcttc gtctgggtgc
    18781 atgattttca tctcgccctg ctgccaggcc tgatcaaagc ctccctgccc aaccaaccgg
    18841 tcgcgatctt ctggcatact ccctggccag gcccggatgc gtttcgcatc ctgcccgaac
    18901 gccgggaact cctggaatca ttactggcgg ccgacctggt gatgtttcag acggaaagct
    18961 ttctgcactg ctttcttgaa tgcgccaaag aattcctcgg caccgatgtc ctgtccgacg
    19021 agcatcacat cgactataaa ggccactgca cgcgattggt cgttcgcccc atcagcatca
    19081 attatcaatc gtgggccgag cgggcacaat cgccgcaagt cacgcgggcc atggacgtct
    19141 tacgtaatct acacattttt cagccgaccg tacgcatcgg actcggcgtc gaccggctcg
    19201 actacaccaa aggtctcctg aagcgattgt gggccatcga cgccttcttc cagcagtatc
    19261 cgcagtatcg cggccatttc accttcatcc agattgccgt gccgacgcgc ggcgatgtgg
    19321 aagcttaccg gagctatcgc gaactgatcc gcgaaacggt caatgagatc aatatgcgct
    19381 atggcacgat ctctcacccc ggtagtccac aggcctcgcg ctggcgaccg atcgaatttc
    19441 gtgaagggcg cattggtatg gataccctgg cggcctatta ccgcatggcc gacctggcgt
    19501 tagtgagttc ggtttacgat ggcatgaatc tcgtcgcgaa agagtatgtc gccagccagg
    19561 tcgaagagaa aggcgtgttg ctggtgagcc atttggccgg cgcggcagaa gaattgaccg
    19621 acgccgtggt gatcaatccc tacgacccgg aaggtgtggc cgactccatc cgccaggcgt
    19681 tggagatgcc atttggagag cgccgtgaga ggatgcgacg catgcgcgcc tatctggagg
    19741 cacatgacat tcgcgcctgg gctgatgact gcctgcgcga cgcaggaatc cttccacagc
    19801 aggacagtcc cgcccttgaa taatcgacgc ctgcccgcaa ccaccgatcc gaagatcccg
    19861 ttacgccgac acctcccgtt gttcctgttc ctgagcctct cgatgctcct caacggctgc
    19921 acacagctat ccaaactgtt cgcggtcacg ccgtcgccag atcaacaagt ggatcgccac
    19981 caacaggcga tagtatcccg gctcgcgcca gtcatcgaag ctgaactgca agacaatctg
    20041 aacgtcatgc taccgacagt tcgcaagagc ctcgccgatc agacgaacaa ggtggcgaag
    20101 ttcaagcgcg atctggtgtt gcgttcgcta tctgatccct gggcgggcct cacctcgatc
    20161 gagcaaacca gccttgtcgc cgccaccgcc gccgagtctg gaattgagga actgcccgcc
    20221 atcatcgaca ttttggaaga agggatggag cgaagcggcg cgccgttcaa gcccctcaac
    20281 tttccggcta ccctggcacc ggaggatatt ctcggtttta tcatggaagc gcttgaagat
    20341 gcccatcagg accgcgaaca ggcactccgg cacctgtctc cgcaggatcg ggactttctc
    20401 ttcacccagg cgcgtccgct tgcggaacaa ttccttccgc aaatcattcc gccggccagg
    20461 cttgcggaag ccgaagccgc cggccgctat gccgcgctgc tcatgcaaca ggtggattac
    20521 gcatcgctca ttgccgccgc acaacgatta gcacggctcg gcaaccggaa attattgcaa
    20581 caactggaca ttgcgctcca ccatcgaaag ccggtcaccc agacggttcc cggcatcacg
    20641 ggcgatctgc tgctggttca gcagacttcc tacggcttac tcgtgatagg cggccatggc
    20701 ccgaatacgt atgacctcga caagggagcc gcgctcatca tcgatctggg gggcaacgac
    20761 acctatcgag gcctcatcgg cgcaagcccg aacagcgacc tgggcaacag tgtcgtcatc
    20821 gacctgagcg gcaacgatct ctaccagccc gccccgctgg gcttagccac tggacgcgcc
    20881 ggtgtcggta ttctcatcga tcatagcggg gacgatacct accggttggc acctggctcc
    20941 ggtggcgccg gtttcgcagg gctcggcatc ctgtatgatg gagaaggaca ggatgtgtac
    21001 gaaggaagcc ggtttaccca aggcgcagcc ttcggcggat tgggtctgct ggtggatcgc
    21061 gctggcgatg atcgctatac gagtttcggg tacgcgctag gattcggcgg gcccttgggc
    21121 gtaggggcgc tcatcgatgt ctccggcaat gattcgtacg aatgcggcgg aaaattcccg
    21181 agcgcttaca atgaagcgga ctcgccgcat gctcgccctc aggatccggc ctttcaatat
    21241 gattgtttcg ggctcggcac cggggccggg cttcgcatct acagcaagca gccggcgcaa
    21301 cgggcgcaga gcctcgccgg cggatggggc ctgctggtcg atctggccgg tcgtgatcgc
    21361 tatcgcagcg ccaacttttc gcaaggacac ggctactttt tcggccttgg cgtgaagctc
    21421 gatctggccg gcgatgacga acatcaggcg gcgcggtacg gcataggcac cgccgcgcat
    21481 ttcggcgtag gcctgagcct cgactatcaa ggcaaggatc ggtacggttc caaaggcccg
    21541 ttctataacg gcggcacagc ctgggatggc agcgtcgccc tggccgtgga cggcggtccg
    21601 gacagcgatt tgtatgacct gcccgcttcg accggcctgg gaatggcaga tctgggcggc
    21661 ttgggcctgt tcattgaaga aggcggcgcc gaccagtact tcatcggccg gggattagga
    21721 cagggcgcag atacgagcat cggcgccttt ttcgacttgg aaggtcggga tgactatacg
    21781 tctgttccgc tgccgaacgg tgatgcgcga ccggaacggc tgaatcgcaa aacctatctg
    21841 gaaaataccg gcagtctctt cgtcgaccgt taaccggtag ttttgccccc cgcttgactc
    21901 cccttcctcc atggctatct caaagaacgg tgatcaacgg gaacgaacat cattccttgt
    21961 agccgattca tcttccttgt gatgaccacc tcagaaccgt ggaggacgat atgactgatg
    22021 cgaacgcccc catactcacg aacctgccga tactcccgtt gaaacgaacg gtcctcttcc
    22081 ccggcacgat gatgccgttg acggtgggac gcgaccgttc catcgcggcc gtcgaagcag
    22141 cgctcaaaac agaagacaag accttgctgg tcgtggcgca acgggatgct cagactgatc
    22201 agccgacgct cgaagacctg tatacgatcg ggacgaaagc cgtcatcaag cagacggctc
    22261 gaaccccgga aggtcattat aatattttga ttcagggact ggagcggttc gtgctgctga
    22321 agctggacca ggttgatccc tatctgcaag cgcgagtgaa gcaattgcca ccgcccagcg
    22381 aacggagtac ggaggtcgag gcattgcatc gagccatcct cgacatcatc accgaactgc
    22441 ccaaactgat ccagacacca ggcgtgcatg aagcggtggc ggccctgggg accgaagaag
    22501 accccgtggt gttggcctac cgcatcgctt cgttgttgaa tctcacgctg gacggcgaac
    22561 agcaactgct ggaagcgtcc acccgtgcgg atctgttgcg cgggctgtac gccgcgctgt
    22621 cgcgcgaggt tcagattctg caattgcgcg acaagatcac cagcgaagcc agggaaaaac
    22681 tgggcaaaac ccagcgcgag tacctgctac gcgagcagct gaaaacgatt cagcaggagt
    22741 tgggcgagag caacggcgaa gaagacgagg tggcggcact gaggaagaaa gtgcaggacg
    22801 ccgatcttcc tgagcatgtg agaaaagaaa ccgatcgcga gttgacgcga ttggccaaaa
    22861 cgcccagcgc ctcgccggag catcaggtca tccggagcta tcttgagctg gtgctcgaat
    22921 tgccgtggaa caaatcgtcg gaggaaggcc tcgacctcgc gcatgtgcga aagattttga
    22981 atgaggatca ctacggcatt aaggaagtaa aggaacggat tgtcgaacat ctggccgtgt
    23041 tgaaattgaa cccttcggcc aaggcgccga ttctctgcct cgtcggccct cccggcgtgg
    23101 gaaagacgag tctgggacaa tccattgcaa aatccatggg ccggacgttc gaacggttca
    23161 gcctgggcgg cttgcatgac gaaggggaat tgcgcggcca ccgccggacc tacgtcggcg
    23221 ctttgcccgg acgtattatt caagcgatgc gacgagccgg tgtgaacaat ccggtcatca
    23281 tgctggatga ggtggataag ctggggcgtg actttcgcgg cgatcccgcg gcggccttgc
    23341 tggagattct ggatcccgcg cagaaccata cgttccggga ccattacctc gatctgccgt
    23401 tcgacttgtc gaaggtgttc ttcattacga cggccaatac gctggagacc ctctcgcagc
    23461 cgttgctcga tcgcatggaa atcatccggg tgaacgggta tagcgaacgg gaaaaacggg
    23521 agatcgccct gcgttatctc tggccgagac ggctgaaaga ggctggcctg aaagcagaag
    23581 aagtcaattt gcccgacagc gtgctcgacc atatcatcgg ccggtacacc agggaatccg
    23641 gtgtgcggca actggagcag atgttaggtc gcattacgcg gaaagtggcg gtgacctttg
    23701 ccgatcggcc gtccgattct cctgccgcgc cggtcgaaat cacccaatcg ctcctggatg
    23761 aatggctcgg tcaggaacga ttccagccgg aggaggcgcg caagaatctc ccggctggtg
    23821 tcgccaccgg catggcctgg acgccgacag gcggcgatgt gctctacatc gaaacgaccc
    23881 tcctacccgg cagtcacgag ctgacgctca ccgggcaatt gggagacgtg atgcaggaat
    23941 cggctcgtgc ggcacgcagc tacctgtggt cgcatgcgga gagcatggga ctggacatct
    24001 cgcgtttcaa acggaacggc gtgcacattc acgtgccctc cggcgccatc cccaaagacg
    24061 gcccctcggc cggtattacg atggcgacag cattggcctc ggcctatgtc ggcaaaccgg
    24121 tccggagcga cacggccatg acgggcgaaa tcagcctgac cgggctggtg cttcccgtcg
    24181 gcggaatcaa ggagaaggtg ctggctgccc atcgcgccgg catccggcgg atcattctgc
    24241 cgaaggctaa tgagaaggac ttgaaggaag tgccgcaaga agttcgggat gaactgacgg
    24301 tcattccggt cgaacggatc gaagaggtcc taccggcggc gttcaaccag gatgcggcct
    24361 cgtcgccgga gaaacacgaa ccgatggcga cgtccaccgc atcataagct ctcgggcccc
    24421 ggctgagaag ccggggcccg ctcagcgacc gctttcaaac tccctctacg cgttgaaggt
    24481 cactactcgc ccatccgatc gccttgccct tccgtaaaga catatgtcag gatgaaaaac
    24541 gtcacctggt tgatgcgcac cgcacatgac ccggcaaagg agaggatcca tgccatcgaa
    24601 catcgagatc ggaccgatcg tgaaggtgaa caagaccgac gacgaatgga aaaagctgct
    24661 gccggctgcg gcctaccgtg tcctgcggca cgaagatacc gaacgagcgt ttaccagtcc
    24721 gctgcatgac aaccaccagg ccggcatcta tcactgcgcc ggctgcgact tggccgctta
    24781 ctcttcagac cataagttcg atagcggcac aggttggcct agtttctggc aacccgtcga
    24841 tccgaaagtc gtggagacac gcaccgatac caagtttttc atgacgcggg tcgaagtaca
    24901 ctgcgcgcgc tgcggcggcc accagggcca tatttttgaa gacggtccca aacccacggg
    24961 attgcgatac tgcatcaacg gcgtctcgtt gaaattcgtt cctgcctgaa cacgatccgg
    25021 gcatgtctct gggcgacgac ctctgctgtc gtcgccgatc gtgaacatct ctcgagcgtc
    25081 tcatcgcacc ttgcaattta tcgctgaaca ctcctagact gtgaattcca tggcttctct
    25141 cccttcacta cgctcgacga tggccgccct ttggctgggc gtcacactgc tcgggtgttc
    25201 ccaatcgagc gacgtcattt cgaacaatct gaatgcctgc ctcatcgtga ccgaagccga
    25261 ggttgaattc gcgatcggca cgccggtgac cccgggcctg cgccagaatg atcgtcaatg
    25321 tctctatcag gcaaagcgca acccgcaaga gacggtcgtg gtggaactga atgccggtcc
    25381 ggatggggat cgcaaaactc tgtttcagga tcaacgttca aaagccggca accagccggt
    25441 gcccggcgtc ggggacggcg cgttcacgct tcgttctccc atcggaggca ttcagctgac
    25501 ctttttgaag tcagatgcgc tggtgacact ctcgctcacg tccccgaagc aagccaatcc
    25561 acaagcggcc gtcacgaacc ttgccaaggt cgccgccaac agactgggcg ctccgattcg
    25621 tacgactgcc gcggccgcgg agccgggaat caccggaacc ccctcattgt gggccggtga
    25681 atggtacgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc cgcggagccg
    25801 ggaatcaccg gaaccccctc attgtgggcc ggtgaatggt acggctgcat gcccgtcggc
    25861 atgatgtacg gcaaagggca tctctcgctg aatgaacgcg gcacatggtc gatgacgacc
    25921 gccgtcgtca tgccaggcac catcatcgcg gaccgcgggc ggtgggaggc ggaatcgtat
    25981 caggaaattc ttcacggcac ctacaaagta gccggcagcg accggttttc gaccaccggt
    26041 attttaagcg tgacctggga caagctggcg aagaatcaag gaccgaccaa gttcgatccg
    26101 ctcctctggc aatcgttcac tgcgattccc aggaaggtca acgtcaaacg tctgccggcg
    26161 gtcgaaccgt ccctggtggg cacatgggag gggtcctcca agtttgtcga tcggcaggaa
    26221 gattttgtct ggacgatcac cgcagccaac gtctccgagt tcttcaaagc cacgtcacaa
    26281 actggccgca tcgagcagga acaggaccgg ttccgattgg tcgttccgca aagtaaaacg
    26341 ccgcctctct ccgtccgggt cctggcccag gataaaatgg aactgaccga cacgagcggc
    26401 acggtgtcgc aatggaatcg gaatgaaaag ctgctgtcgc gctgttagtc ctcttcagtg
    26461 acaaggccca tcccttccag ggccggcaga ctttttcgca acttgacctg cttttcccgc
    26521 ctccgataga ctaactctat gcgaacatgg ctcatcgcga taatctttct cgccggaccg
    26581 ctgctaccgg cagcgggggc tcctgtcgga gcagccgcag accgccccac ccccaaggca
    26641 agcgagttgc tggacgagat cattccaaaa gaactctttc gccacctcta cagcgaagcc
    26701 ctctccacat tgcacgaata catcgagatg gaaggcaagc tgcccggtga gagttcgacc
    26761 catcaacagg ggggagagtt tcgcctcaaa ctgtttcctc acggcaaatc ccgatcacaa
    26821 gaacacctga gcgcggaagg ctcgtttcgt gtggcacccg aggccgacca accggaattc
    26881 acccttcgat tcaaatcctt caagaaagcc tcgcctcccg cacgcaccaa tgacgacgtg
    26941 atctgaagcc cactcccaca cgcacgcgtc gccgaccttg cccttgacca aggtctgagc
    27001 ggggcataga atacatttct agtacacgat ccgcctcatg tttggaggtc ggagccatgt
    27061 tcttccttgg gacgcagatc cagtgggatg aacatggtag gtgtaccctg ctcctcgacc
    27121 aaacctcttc tcttaagacc gccgagttac aagcatccac acaacacatt cttacgcgct
    27181 cgcgtacctt cgctcttcac caagcgttaa tcactggtac ggcgacacgt gcacagagga
    27241 ctcgcgcgat acccgcgcgg gagcgtggcc tatgtcagga gtctattggg gaatcgtggc
    27301 aggtttcctg gcgatgatag gaactctctt cgcctgtata gacattctgt atggcgcaca
    27361 gggaggctcg tccaattcgt cagcagatgg agtcgatacg tcgaatcagg cggtcggatc
    27421 cgcgtcgtcc ggctcgcgtc gggcagcgtg agggcgtcgc acgtctgata gcctcgtgga
    27481 tgagagacgc catggatgct gtgtcgcaca gagggctcca tcggcgtccc ggccaacaat
    27541 ctgttgttca acaacggagg tgccacatgg aggtcgagat catcggtgcc ggaacaatga
    27601 tcgcgctggt gtggctgatt gcctggtcca tggctggaga gagcgagtcg gaaagacgac
    27661 ggatcacctc ggcgcctggc acgttcgaca cggcctcnnn nnnnnnnnnn nnnnnnnnnn
    27721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27781 nnnnnnnnnn nnnnnnngcc agggtcgtga ggaccgggaa tcgacccaca aattctggaa
    27841 tgagcccgaa tttgagcagg tcttcggatt gcacacatcg cagcacttca ctgtggccct
    27901 cgagttcacc ggcaggagcg attgcaccga accccaaccg cttgggcatc gtccgttgag
    27961 cgatcatggc atccaacccg acgaacgccc cgcccgcgat aaatagaatg ttcgtcgtat
    28021 ccacccgaat gtagtcctgc tccggatgtt tccgcccgcc tttgggcggc acattgcaca
    28081 ccgtcccctc gaccagcttc aagagcgcct gctgcacacc ttcgccggag acatctctcg
    28141 tcagggacgg attctccgac ttgcggctga tcttgtcgat ctcatcgata tagatgatgc
    28201 ccgtttcggc ccgggcaacg tcgtaatcgc aattttgaag cagcttgagg accacattct
    28261 caacatcctc tcccacatat ccggcctcag tcagtgccgt cgcatcggca atggtaaagg
    28321 ggacatgaag gatacgggcc agggtctggg acagcagcgt cttcccggtc ccggtcgagc
    28381 cgatcatgag gatgttcccc ttttcgagat cgacgtgctt gagccgttcc cgattcacaa
    28441 tccgcttgta atgattgtgc acggcgacgg agaggacttt cttggcacgg tcctgcccga
    28501 tcacatattc atcgagcttg gccttgatgt ccgtcggctt ggggatgacc agcggaagcg
    28561 gtgagactga ggattgggaa ccaggagttt tgttcttggc cagggaaagg gagcagcgtt
    28621 gaatacattc ttcgcaaata gaaaccgctg ccgggttcct acaagaggtg cacgcgaacg
    28681 gctctgaatt cgtcacgagc gtccggccgg cgtcctgagg cttgccgcaa aacgaacagc
    28741 gccggtctgg tttctgcgtc gaatgccgcg tgtcccagaa caccgcgttt cctccatgct
    28801 acgagatgtg cctggatgcg atgcctcatc ttatgcctct gtgatggcga cctgcaagca
    28861 tggaggaatc acggtctcag cgctgaacgg cgcgacgagc gcctgccgga ggatgaccac
    28921 agcctgctag aaggtcacgc caaacccacc ggcaatgata tgggtacggt agttcagggt
    28981 gaaagaggga cctcgggagc cattcgtgaa ttcactctcc cattggtaat tcgccacgaa
    29041 atatttgtac tccgtgaaga ggaacaggcg gtccgtgatg ttggcacgaa cgccgccgag
    29101 aaattggtac gcgaaggcgc cgtcggccgc attttcatgc acggtaccaa tcgtgctgtg
    29161 ctgaaggttc aaatctctgg cgaatccacc tgacaatccc gctccgattc cgatataggg
    29221 ctgaatcaca tcgcccggat aacgcagtaa caagttggcc atagcgttaa tcgattgcag
    29281 acggaaatta gcagatctcg tcacgccccc gctcgtcgcc gtcggtgcgt tcacttttcc
    29341 gtcgaggccc gataactccg cttccaaccc gacataccgc ccggcaaacg ccggaaaaaa
    29401 cccgatcttg agtccaccgc ctaacccgcc ctgaacatcc gtattcgtga acgggtcttc
    29461 ttgcaagcgg gcgggacgat tcagcggccg gcttccctgc atgtagaggc tgatgtacag
    29521 ttccgactta gtctcttccg cctttccctc cgctgatgcc gacggcgatt tcacggcatt
    29581 cgccgcaggg tctgctgcga gcaacagagc gggatagacc tgtagcaata caccggcgag
    29641 aacaacgacg cccagagacc agaatcgtga tttcatgagt gactcctccg agtacgtggc
    29701 aagacaggcc cagaacggcc tgcgatagct cactcatatc acgagcaagg tgcgcgacaa
    29761 actgattcgg cagtttcggc agccgtactc tctgtctgta cgtatggtgg aacttcgtac
    29821 gtaacgaatg ggttgtcacg agctggcgag gaactgtgtg agagtttcag gtggaaccag
    29881 acccaccaat acctgtccat cagcccgttg cagcaagggc acgccctgat gttctgtctc
    29941 ttcccagcga tctctccatt ccccgagcat cgactctact gcaggcgcgg ttgatccgaa
    30001 caatgcggtt ccgacgaacc cctggcgttc cgcctccagc cgtaacagac gctcattgga
    30061 aagatccaaa ccatctaccc agaacagacg atacaacgac cggatgaact cgcctgctcg
    30121 ccgagtatcc tcgcgcagcg cacgcgcggc caccgtaatg gctaatctgg tattcggctt
    30181 tcctgccggc actgagatgg gcaactcagg cgcgagccga cggaccattt tcacctcctg
    30241 cttcaactcc gcatgtaaat gtccggccca ggtggccatg ggctgaggca agtgaggggc
    30301 atgctgcaca ccatgccacg atatctgttc gaggaaaccg agcgcataca gccgctcatg
    30361 catcgcataa caaaaggggc agttgaagtc gctatacaca cgacaggagg tttttctgct
    30421 cgtcatttgc cgtcccgtga cggaagaatc gcgatgatgc ggaccggcgc cccggtgcct
    30481 cccgcaatct tcatcggcag cgccaccagc ctggcgcccg atgcgggaac ctctgcgaga
    30541 cccgccacat tctcaagccc gtagaggcct gcggcattga cgatccggtg cacgacaaaa
    30601 tcctgagagg gcccgtaatc gatactggcc gtatcaatgc cgattccgga aatgtgccgt
    30661 tccctgacga gaaactccgc cacttccttg gagaagccgg gaaaatgcag cgtggttgga
    30721 atctccggcg tcgcgctgcc gaaatatcgt tggcgatcat tccaataggc gccccagccg
    30781 gtcagcatca tcacgatggc gcctgtcggg atggtcccat gggcagcttc ccagcgatga
    30841 atgtcggcga gagacaggcg atagtcacgg tcatcggccg ccgctgcgcg cacatcgatg
    30901 accaccgcag gtcccatcaa ctgctcgacc ggaatgccgt cgatccccgc ttggccctgc
    30961 gcaaaatgaa taggcgcatc catgtgtgta cccgcatgct ccgacagggc cacctgtccg
    31021 gtggcatacc acgcttcggt ggtggtgccg cctcgctgcg tggagtcacg atgaaacgga
    31081 ggattgctgg gccacacctg ggtcgtctca tcgaacggat aggtcaggtc gagcactcgt
    31141 ggtcctatct cggcggcatg agacccgctt acggccgaca cgtgcatcac cacacaaagc
    31201 gtcaacgccg acaatccgag ccaacgtaga tgtcttggtt ttatcaccat gtttcctcgt
    31261 cctaggagtg agtgcccccg gagctttcgt acagggcgcg attacggtac cctctcaccc
    31321 ctcagtagcg caacggggaa ggaaccggta tgtcggatca gcagctccat ttttccgggc
    31381 aacgtttttc tgtggaatat cacattcagg gcacgaaaga ggaggcgtgc cggcgcgccg
    31441 ctgtcctctg catcgatcag accgtcgaag ccgcggacca cgtgattccg tcgggtgtga
    31501 ttcgcgatca gctgctcggt cagatgctca gcatcgacag ggtcggagac ggcgttcacc
    31561 gcgcgacgct cacatttccg attgaactcc tcgatggaac catgggcact ctccagcaca
    31621 tgagctttgg catggcgggc ctgcaaagcc gggttcgcct ggccaatctg tcgcttccgg
    31681 atgaggcgat cgcccagatg gctggccccc gcttcggatc actgaaactt cgtgcgctac
    31741 ttgaagtgcc tgtgcgcccc ttggtctgcg ccgtactgaa gccactcggc ctgtctgccc
    31801 gcgcgctggc aagcctggct tacgaattcg cactgggcga agtggacctc attaaggatg
    31861 atcaaagtct cggtgaccat cctttctgcc gattcgagga acgtgtgact ctgtgcgctg
    31921 aggccattgc caaggcttcc cggcaaaccg gcaggcgctg cctctacgcc ccacatgttt
    31981 ccggcccctg gccgacgctg ctcgaccggg cctccctcgc gcagaacgcg ggcgcgggtg
    32041 cgctgcttct ctgtcccgga ctcacaggct ttgacgcact cggcactctt gcccgtctcc
    32101 cattcccctc attacccctg atcgcgcatc cggacttcca aggcagtcac ttcgtcaact
    32161 cagccaccgg tatcgccccg gcagtgctct tcggtctttt cccgaggttg gccggggctg
    32221 atgtgtcaat ttatcccacc ttcggattgg agtatccgat ctcgcaagag gattgtcggg
    32281 gcatcgccgc ctgctgccga aacagcctcg gcgcctgtgc cccgatcttt ccgacggccg
    32341 caggaagaat ggaggcctcg cggatccatg agatggtcgc cttctacgga accgatgtgg
    32401 tctttgtcct gggcagcgac ctacgtcgag atccgactcg catccgaagt gcctgccagg
    32461 aatttattca gcttctcgaa gccgaggtgg ctccataact cacatctcca cttctactca
    32521 atgtattgca tgcaacatgt tgttatgcat catgttttat ccattaaaaa acttgcttgg
    32581 cactcatctt ttcctgaccg tcttcattgc cattggcaag atacccggga aggtcacagg
    32641 gatatcccct tgattcatca agttttctat gccggccacc gatacaggcc ataacctgaa
    32701 gtacgtatgc atagtgagcc tccgtccgtg tttgggcaat catggcctta gctaaaacat
    32761 ccgacgactc cagaaaactc gataagtccg acaccctcgt gctcagcccc gagcgccttc
    32821 gtgtggggat gtatgttgat ctgaattgcg gctggttcag acatcccttc ccccgacgct
    32881 cgtttaaact cagctctgcg agccagatcg ccacgatcaa agggcttgga ctcactaccg
    32941 tcctggttta cccccgggaa tcagatccgg acccggacgc agagggaacg ctgctagaaa
    33001 cggatccggc gtccacggcg actgtggaac cggatacggg atctgctccg gcagcggccg
    33061 ctgccgatac gaatcctccc ccctcggacg aagagcttcc cacgcatggc gactatcatc
    33121 aggccgtaga attggcttct gaaacctacc gccagatgct cgggaccagt aaagacatga
    33181 tggacgcgct cacaagcgga tctgaggaag gcatgcgttc cgcggaaacg atgatcggcg
    33241 gcatcagcac gctcttgacc catacccagg ccgccggcac cgtggcgagc gccttcgacc
    33301 ccgaagaaat cggcgatgcg ggtgtgctcc actctctgaa tgtcgccacc ctctccatga
    33361 tggtcgccca gcaatttgac atcgaccagg acgccctaca gatgatcggc ctcgctggcc
    33421 tcttgctcga catcggcgag cggcgtatcc cgcgccatat tttggataca cgcgcgcgac
    33481 ggtccgaaac ggaacatgtc aaataccagc tccatcccta tctcggcgtc gagatgctgc
    33541 gagggtttcc tggaattccc gaagaggtgc tggacgtgat ccgcagccac catgaacgtc
    33601 tggatggatc cggctatcct gaaaaattga aaggggacca gatttcattc ccgacgagaa
    33661 tcgtcagcgc cgtcgatcac tacgatacgc tggtgaacag tctctacgcc gaagaggcgc
    33721 tgtcaccggc cgaggcgctg gcgaccatgt acaagcatga aaaacagctg ttttcgaccg
    33781 aggtcgtggt ggccctgatc cacacgttgg gcgtctatcc cccggggacc gtcgtgtcct
    33841 tgtccgatgg gtcattcggc ctcgtcctga acatcaactt caagaatcgc atgaagccgt
    33901 tggtattgat gtatcatccg actccgggtc aggaacccca tacaatcgat ctgctgagag
    33961 accaaacgct cagcattctc cgtgccgttc cccgcgagga actcccccgc ccgatcgtcg
    34021 aattcttccg gctaaaacgg tggaccgggt atttcatcca ggccaccatg cgcaccgcct
    34081 gatgcactga ggattgccaa gcctgatcac tggcagcggc gcggtgcagt atggtatctc
    34141 cctggcatgg ccctaccccg tgtgctcctg atcattcccc cgttgacaca attgaacacg
    34201 ccgtatcccg caacggccta tctcacaggt ttcctgcgat cgcgagggta tgaggcggag
    34261 caggctgatc tcggtctcga gatgatcctt cgtctcttct cgcgagttgg acttcaggag
    34321 gcgtttcacc gcatcaaggc cagccgaaga ccttggcgcg gagaggtgca gcaattcctg
    34381 gcgctcgaag acagctatct ggccaccatc gacccggtca tcgcctttct tcaaggaaag
    34441 gatccgatgc tggccccgca tctcgctcgc ccgggattcc tgccggaagg tcctcgcttc
    34501 ggcgccgacg ggcgaccggg atcgagccct ttccccactt ctccaaccga cagcgcccgt
    34561 caccgcgcga cgctgtacct ggaagatttg acggacctcc tctacgaaac catcgcgccc
    34621 catttattcc tgaatcgtta cgctgaacag atcatgcaag cgacgtcctc cttcgacggg
    34681 atagaaaagg ctctggccga accactgagc tttaccgacg aactcctgct cgaggcgctc
    34741 gcgccccttc tggccggcag ttcgccggat gtcgtgggtc tcaccgtgcc gtttcccggc
    34801 aatctcttcg gtgccttgcg gattgctcaa gccatcaagg cacagcggcc tgcgactgcc
    34861 gttgtcctgg gcgggggcta tgtgaacacg gagctccggc gtctcaagga tccacgactg
    34921 tttcgatacg tggactacgt cacgctggac gatggggaac gcccgttcct ctgcctgctc
    34981 gacttcctgg ctggtggacg gtcacaaccc catctctgcc ggacatttgt gctggccgac
    35041 gggcaggttg tatttcgaaa cggcaatacg gagccggacc tatccatgca cgaactcggg
    35101 acgccgacct acaacggact cccgctcggc cgttatctct cgatcctcga cagcctcaat
    35161 cccatgcacc ggctctggtc ggaaggccat tggaacaaac tcaccgtcgc gcacggctgt
    35221 tattggaaac aatgcacgtt ctgcgatgtg gggctgaact acattgcacg ctatgagatg
    35281 acgcccaccg atgtcctgat caggcagatc gaggccctga tccgagagac aggccagcgc
    35341 ggttttcact tcgtcgacga agccgcgccg ccggctgcac tgaaagcgct cgcacttcgc
    35401 ttgctcgaaa cgaatcagac catttcctgg tggggaaaca ttcgcttcga ggaagcattt
    35461 tcaccggacc tggcaagact gttggcggct tccggctgca tagccctcac ggccggactt
    35521 gaagcagcct cagaccgttt actccggcaa atgaaaaagg ggattaccgt agaccaaacg
    35581 gccagagtcg ccgcggcatt ccgccatgcc ggcattctgg tccacgcgta tcttatgtac
    35641 ggcctgccgg gtgagagcat cgccgacacg gtagactctc tcgagcgtgt ccggcaatta
    35701 tttgcaaaca atttgttgca gtcggcattc tggcaccgtt ttaccgccac cgcccacagc
    35761 cccgtgggac tcaatcccgc cgccgagggt atcaccatcc tcgggccaac atttgaaggg
    35821 tttgccgaga acgatcttcg ccatgccgac ccgaatgcct cctgcccgcc atggcttggc
    35881 gaggggctcc gcacagctat cctcaattac atcgaacagg cgggactgac cgtgccggtc
    35941 caacaatggt ttccacaagc cgtacaagct cctcgcgtgg cgaaaacatg gctgacacgg
    36001 gccctggccg acgccgtccc tgaaccattg accgccgagc ggcggtgcgt ctggatcggt
    36061 ggacggcccg tcgtggcggc aaagggcacc acgcgcgtga agattattct gccgacccgc
    36121 atggaagact tctcgatcag tgtctcctca gatcaggtcg actggctcat ggatctattg
    36181 cagaacgcca gtccctccct gcaccgcaca gaccgcgact acccccgctt cgccgaggcg
    36241 agggagcggt ttccccacac cggcgcacgc ggatttgatc ggtttgccag gactgctgcc
    36301 tggcataacg ctcgccaggc tgggctgctc ctggtctgag tcgaagccat tcgcattcgc
    36361 ggtccactcc ctacctgttt attcacaatc agaacggcga cgcctcctac cacggagcac
    36421 tcgctgcatc attctgagac aggtgttcga tgctgatacg gaatctgagc atccgtgtag
    36481 tacgtattag tattttgatg tagcgtgtca cgaaatgcga attacgcgtt gacattcata
    36541 cgtatctcaa ctacatgtgt tctctacgta tcacatattt tacggaggac acgtatccat
    36601 gatgactcct cgattgctaa cccttttgat ggcgaccggc atcgtctgcg ccgcgccact
    36661 ttcggcttca gccatcgccg tctattcatc ctccggctcg aatgccgcgt cgattcaagg
    36721 ttctgtcgat ggatttcggg ctgatctcgg gacaaataac ctggctaatg ccccggcaac
    36781 cggcggacgc cgcgagatta attgggatgg agtgcctgct ggtttctctg acccgaatca
    36841 cctacccggc aacttcttca actccaactc gccacgtggc gccgtgttcg agaccccggg
    36901 gtcgggattc aaagtcagcg caaacaccgg cgtcggaacg cccgaacgtt tcggcagcat
    36961 caatccctcc ttcccctcca gcttttccgt cttcagttcg cagaaactgt ttaccgccat
    37021 cgatagcaat attgtcgatg tgaagttctt tgtgcccggc accactcagg cggccaatgt
    37081 tcgcgggttc ggttcggtct tcacggatgt cgatctctta ggcccgacca cggttgagtt
    37141 cttcgacatg aacaataacc tgctctcgaa gcagaccgtg ctcaatacgc ccggcgccac
    37201 aagcctgtcg ttcctgggtg tgtcactgga gcaagccatc atcaatcggg tccgcatcac
    37261 cgcgggaaat gccgccctct cgaatttaga cggcttcgac tttgtcgcga tggatgattt
    37321 catctacggc gaaccgcaag cggtgccggc tcccgctgcc gtgctgttgc ttgcaagcgg
    37381 cttggctgcg ttagtgtggg cacggcggaa ttctctgctc ccggaatagt tttcagagat
    37441 tcgcctctct caaagacaag gggggagtcg gtactatgcc gactcccccc ttgtctttct
    37501 tcctcaaatg tactgactca agctgctcag gcgctcaaac gtcaagagga gccggtctgt
    37561 cgaccgactc ctcttgctgt gcgttcgaaa tgcagtgact gctgccgcta cttgttgggt
    37621 tgcggtgtca ggcgcagata cggcttcacc gacttgaagc ccttggggaa caaggtcttg
    37681 gcttccgcat cgttcactgc cggggtaatg atgcaatcct gcccgtcttt ccagttgacc
    37741 ggcgtcgcca ctttatactt ggcagtcagt tgcagggaat cgaccacccg cagcaattca
    37801 tcgaaattcc ttccgcaaga cgccggatag gtcagcatca acttgatctt tttgtcaggc
    37861 ccgatgacga acacggatcg caccgtcatg ctgtcgagcg aattcgggtg gatcatatcg
    37921 tacaggttgg cgaccttctt ttcaggatcg gcgatgatgg gataggtcac ggtgcagttt
    37981 tgcgtctcgt tgatgtcctt catccacccg cgatgcgaat cgagcgggtc cacgctgatg
    38041 gcaaggattt tcacgccacg cttatcgaat tcgcccttga tgcgggccat gtagcccaat
    38101 tccgtcgtgc agaccggggt atagtccttc ggatgggaaa acaaaatccc ccacccgttc
    38161 cccagccact catggaagtt aatcgtcccg tcagtggttt ccgctgtaaa attcggggca
    38221 tcgtcgccca atcgtaatcc catgatttcc ctccttaggt atcccgtcaa acgtggtgaa
    38281 ataactgaca catgaaagcc gacaatggcg taagactacc gtttctgagc ggcgaggcgc
    38341 aagcaggaag ggtactgaca tgcactgtcg gataatcacc cctctctcaa gtttccgctc
    38401 gtgcatccga taagggacac aggtgccaaa ggagttcgca tggacaacat gagacaacct
    38461 gcgaccggac tcaggattgc caacgatccc atgtaccggt tactccgcga ggggtgcatc
    38521 aaggaattta atgtcaaacg tgcggcgggg gaaccggtcg acctcacgcg ttgcgacctg
    38581 cgcggcctgg atttgcgcgg gctggaagtc gatgggttgg acttcagcga atgttacttc
    38641 aggcaggcgg accttcgcgg catcgatttc cgcaaggcca agctggaagg cgccagcatc
    38701 aacgccgcca aaatctcagg tgcctacttc cccatcgaat taaccgccag cgaaatcgaa
    38761 ctgtcgctcc tgcacggcac gcggatgcgg taccagccca aaccctagcc tgcaccgatc
    38821 agtcagcttc cggtcaaact gagatgcacc cgaatctgcg gcttgtccga tgccgccagt
    38881 tcggcctcga ccaaaaatac ccgttcatcc ggcaagtgtc cctgcgcgat cagatgttcg
    38941 cggatggctt tcgcccgctc ctgggccaaa ccacgcagtt cagcatcctc taccgtcatg
    39001 gccggaaaga gctgctgccg gagttcgtcg gccgtcaaca cccgctcaac ggattttcct
    39061 ccgggaagct cctcccgctt catgggctgc ttgcccaact tctccgcata caaatcactg
    39121 agcagttcga attcccgcga gggagagggc acggcttgca ggtttttcgt ccctcccttc
    39181 gtaaaccgcc gcaggacttc gtccgcaatc ttctgcaggg caagggcctg tcgatctcgc
    39241 acgggatcag ccactcccgt gacatctacc cggagcgccg gccgctcttc taaggctttg
    39301 gcaattgact ccagcttccg ctgttctggc tccgcaaggc tgtcgcttcc cgcctgaaac
    39361 tccacaaact gtaggtcctc tcccccgccg ccgacgagtc cccccaaggc cgaaaacgga
    39421 gaggtgacga ctttggtgag caggttcacc agggcgttca ggacgacccg gccgtaccgg
    39481 aaatccggtt cgttcaaatc tcctcgcacc ggcatatcga tatcgatccg gccccgccgg
    39541 tctttcaaga gacccacggc caagcgaacc ggcagcgtcg tggcatccgg gctgtcggtt
    39601 ttgtctccga acgtcaactg atcgaccagc accttatttt ccccgaccaa ttgcttcttg
    39661 gagatctgat acatgaggtc cagcgacagt ttccccttgg tgataggata gcccgcgaac
    39721 ttccccgcat agggtgacac tgacgtcaaa tccacaccct ggaacacaaa cgtcagatgc
    39781 gtaaaggtgt cttcactgag tggattgatc tggccctgga tcttcagcgg ggcaacctcg
    39841 tcgactttcc ccgtcaaggc cacctcagcc ttggcgatct gccgtgaaga gaggcctttg
    39901 atggtgccgg tcaggtctgt gattcccgtg ctcacagctg gaacgatcga ttcatcgctg
    39961 aacgtcgccg acaagttcgt caacctgacg acagcgatct caacgggagt cggcgtcgcg
    40021 ggcttctttg gggcctccgg ctccacgggc ttcgatgctg gtgccggcgg ggtatcggat
    40081 ggacggagta ctcgtgacag gttggtactc ccgtccttat cggtgatcag ccggaacgcc
    40141 ggatctcgca acgcaatctc gccaattttt accgtggtgg gcgccacatc cagcgccatc
    40201 ttgctcaggc tgagcgctgt ccacccgaga aactctttcg tcgaggcgcg gtcggctacg
    40261 tgaagattgt tgagcccggc ttggcctctg tactgcaaca ttggctctga ttcgtggcgg
    40321 ctccgataga cgaggtctcc gtccaacatc aattcaccgt ccctgacctc gagctgaacg
    40381 aaccgatcca gatagggttg gaagggccgg agaccgatat gatcgagctt gagcgccagc
    40441 gccgcttgaa gggaatcgag gcggagtgtt cccttggctt ccaaggcgcc ctgctgattg
    40501 agtcggaaac cggccgtgac cggcaccgcg cccttaaatg gcacatgtac gtcgcccgcg
    40561 gtcagatgca catcgtcgag cagcagttcg accggcgtcg cgaggcttcg atcttcaaag
    40621 gccacctggg tattcgcgac ctcaaccgac tgcaccgcca ctttccacgc ttgacctgca
    40681 tccggcgcag gaggctcaga cggtggcgaa gcgtccgccg tggcggcaac cggcgcaaac
    40741 aggggttgaa agttcaccac cttatcctgc ccgagccacg cccgaacctc cgctccggtc
    40801 aatgccacag tgccgattcc caactcccgc ttggacaaat ccatgttgat gccctctatg
    40861 ccgaacgagg gcacggtcac caccggatct cgactccccg cttcagatag ccggaaatcc
    40921 gccaactgca ccttgccctc ggtcacctgc aagttaatgg gggcaagatg tgtgtcgaag
    40981 tgatatcgga catcgaccaa taatgtccca ctcaagatat cgaatcgaaa ccggtcacga
    41041 agactcggcc aaaacgtttt taagccgaca tgcgagagag agacatgtcc atccgattcg
    41101 agtggatcga ggtgcaaggt gccctcccag gccagcactt caccttcccc gaactccgcc
    41161 gtaaacgcgt aggcgttttc gcccctcccc ctgctggtga agttccgtac cgaaatttca
    41221 atgggcacga catcgatggt caccggccgt ttcttggaat cgtcccggta ctcaatcacg
    41281 ccacgctgaa tactcagctg ccgaatctct atggacggca tcggttcttt acgaggctgc
    41341 tccaccggtg gtgcaggccg ctcggcctct tgggaaccgg gtggcaccag gccaagaaga
    41401 ttaagcttcc cgtccgcctg cagatgcacg aggccaaacg gaagcgtgag acggatctcg
    41461 tcgaacaggt atgacgagcg aactaacgag gtggcttcaa agtctacaaa caattcctga
    41521 aacccgatca tcggggtgtg gtccggctcc tgaatctcaa aaccggtgag ctcaatggaa
    41581 aaggtaaacg gattgagccg catatcgccc aagacgacgg gatgaaccaa ccgttcagat
    41641 aaagcaggaa tgccgtaggt tttgactgcg tagggggcga cgacaaaacc tagtagcgca
    41701 tacaacgcca ccaagccgct gagcacatac aggagaagtc gatagcggga gaggaacgtc
    41761 atgtcaggca cacatcagga aggtgggggc accggctgga atcgagccag caaccctctg
    41821 cttgcaaagt cctttgaact gcagcgtgag taaagggatc gaatgggaag gataactact
    41881 aagaccgcct agaacaagga gaaaatgtct catggattac aactacgtgc catccagaca
    41941 ctgtcaaccg gggagaaaca tcggtaatgg agcgaatgtt gagggcttgt atgcaccatg
    42001 tcgatttgcg aaccggacga cgactactag gtgaagagtg gaatatacaa gaaaggagaa
    42061 atcatatgcg cttcttagta ctcatgatcc caggcgtcta tcagcccaat actaacaggc
    42121 agccgaaggc caattttacg cctactgcag agatgatggc gaagatgggc aggttcaacg
    42181 aggaactcaa acaggccggt gcgctgcttt cattggatgg actgcagccg ctcgcgaccg
    42241 gcgcccgcct ggcattctcc aacggtaagg ccagcgtgac tgatggtcct gccgtggacg
    42301 ccaaggaagt ggtcggaggc ttttggatgc tgcaggctac atcaaagcaa caggtcatcg
    42361 attggatgac acgctgcccg gcgctggatg gcgacgtgat cgaaattcgg caggtcttcg
    42421 aattgtccga ttttcaggag gatgtccagg caacggcgaa aaaacatggt tgagtggcag
    42481 cgggttgcag aactggacgc aagagtgcct atcctgctgg gagcctgtga ttcacgtaaa
    42541 accgaaacat gaaggagaga acgatatgac caagccagca aagaatacca tttgcctgtg
    42601 gtacgaccgc gatgctgagg aggcggcgcg tttttacgcc aagacctttc ccgactcctt
    42661 cgtgggcgcc gtccataggg cacccggaga ctatccttcc ggcaagaaag gcgatgtgct
    42721 gacggttgaa tttaccgtga tgggcattcc ctgccttggg ctcaatggcg ggcccgcctt
    42781 caagcacaac gaagcattct cgtttcaggt tgcgaccacc gatcaggccg aaacggatcg
    42841 atactggcag gcgatcatca acaacggggg cgaagaaagc gaatgcggct ggtgcaaaga
    42901 caaatggggc ctgtcgtggc agatcacgcc ggtagtcttg acggacgcgg ttacgaattc
    42961 cgatcccgcc gtcgccaagc gcgcattcga tgccatgatg caaatgggaa agatcgacgt
    43021 cgcgacaatc gaggcagc
//
LOCUS       DAOQDS010000036        42397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_292_length_42397_cov_19.506920, whole genome shotgun sequence.
ACCESSION   DAOQDS010000036 DAOQDS010000000
VERSION     DAOQDS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 42397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42397
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_292_length_42397_cov_19.506920"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..447
                     /locus_tag="PK589_10520"
     CDS             67..447
                     /locus_tag="PK589_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20079.1"
                     /translation="MWKKNFLFRAHEAAPLKESENELFHDDAEPALDSAGLQMEKFLS
                     VWVQGEGEDEKPTMYTNMYVRTATLDFKTRAGFLQPLQGRTHQIKQMLTPEQKGFLRE
                     WLSTTSPQAWEDSDDHFRTLFDLE"
     gene            662..1207
                     /locus_tag="PK589_10525"
     CDS             662..1207
                     /locus_tag="PK589_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNJ20080.1"
                     /translation="MSHPTSLPLMALIVAGFMFLSQGPVCASATIEVYYAPEDHPIDH
                     VVNLYGRATRYIYVSVYGLTAPSIVKALVEAKHRGVDVRVITDRERLTDPKQHSAVKT
                     LRLAGVPVRINRHDALMHLKQVVIDDEINTSGSANHTTSGNRYNDERLDVITDARLTA
                     RAREKFLAMWNDRERFVVWTE"
     gene            1216..2493
                     /locus_tag="PK589_10530"
     CDS             1216..2493
                     /locus_tag="PK589_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNJ20081.1"
                     /translation="MARASIETDVAIVGAGGGGAVLALMLAQQGVRSVVLERASGPPQ
                     GLRGEILQPNGQRVLDRLGLLDKLPAHAVRSVHRFNFCRSGGERLCTVDYSDLPAPYN
                     RAVVTLPNVAHHAILDALEKQNPGGLWYDATFTGLRFDGSRVVGLQATRHGEPVEVSS
                     RLVVGADGAFSKVRESLGISADIHRYPESYLIAILKAPTGFDEARYLVGKREILGLFP
                     AAGQQVYAFYMIKAGSYDAVKAQGLESLRRAWVRIDPAMESIVSGLVDWNQTGYMPTG
                     RVRTDRWVADGAVLIGDAAHAMNPHASQGRMQAMVDAVTVADLIPGWLKNNTFSSEAL
                     RAFEVARRPQVSMLQRLADEQCRFWNTGNPLLAFLRDRVFRTLDRNARLRYRVLTTTA
                     GLRSQPPFSMLDRVMAAGLLPDPSADRKSSVGT"
     gene            2696..3454
                     /locus_tag="PK589_10535"
     CDS             2696..3454
                     /locus_tag="PK589_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20082.1"
                     /translation="MTQPTALDVPSEVQQLLQRYVKETTTLLGPQLEGILLYGSAVGG
                     EFLPGRSNLNLLLMLSGYDREGMKRYAKAHKRWSREQFVVPLLVTETELTKPGTVFPL
                     EYLEIQEQHRVLWGKDPFIGLHIDRTHLAYQVQQGIQGNLVRLRQRFIEGGGTEEATT
                     MLLPLSLTALLPCLRGLQRLAGHPALFQSDALLTAIRHMTGLDLAGLGDVLELKRGII
                     SPGPVEVPRLYERYAGNLEALIALIRPDGTVAPR"
     gene            3451..4188
                     /locus_tag="PK589_10540"
     CDS             3451..4188
                     /locus_tag="PK589_10540"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNJ20083.1"
                     /translation="MKKRSLTINGLIPALWLLCVVMAAPLGHAREPVPNYDPQGYVSD
                     HAGVIEADWRARIRSVCQDLERKTGVEMVVVTVPTLKPYPSANDYAMGLYQKWGIGSA
                     QDEHGVLILLAVQERQAAVTMGRRMIPVMGGEVVGKVGHEYLDPAIKNGHFDEGLYRT
                     VVALASVSQEIRVGSTKKAHFRGLGFWITVTTASGALWFLWWLSRPDLRHPFGRIRKG
                     QYWGTGQGGFGGNFGGMGGGMSGEGWK"
     gene            complement(4330..4716)
                     /locus_tag="PK589_10545"
     CDS             complement(4330..4716)
                     /locus_tag="PK589_10545"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ20084.1"
                     /translation="MKDETIPAAVRQYFEAIGKMDADSWVSCFAEEGISYEPGAPAPL
                     KGRAALRQFLVGVLGAFETIRMTADHVFQSANRVAVKFTGRGTGKNGRPVFFEGIDVF
                     EINQEGKIQTMWGYWNPATMMTQLQG"
     gene            complement(4908..5465)
                     /locus_tag="PK589_10550"
     CDS             complement(4908..5465)
                     /locus_tag="PK589_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ20085.1"
                     /translation="MTTTAPHDQSRFAPGHTSNRSVSPKNILIAVDDSNATIRVLHYV
                     GALLRETPSVAITLFHVLNPMPRELMEHGGSENPDMEHQLGEQLRKDQQEWMQMEETL
                     EYPILERALERLKDTGFPTERVMLKLGYAGDLVDTILDEVRAGGYGTLVITKHSQPDA
                     AHLFSNNVAERLSRELSGIALWVME"
     gene            complement(5770..6366)
                     /locus_tag="PK589_10555"
     CDS             complement(5770..6366)
                     /locus_tag="PK589_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20086.1"
                     /translation="MALVQQNLVPGTGDHLITLDTETNLEWLSLNATANLSYMDVLGG
                     AGGYTTTYGFRYATGQEISLLHQHAGITKFGLTQVVPFPQSNHVAMETLIELMGGVAL
                     YPSVASGSVLVQTQGMMKFRGTGIPAPMTPMSVVQLWLFKHNPPSSYADSNPAGPAGN
                     RAPEIASYLIRNRVMPVGVVARGKSAKTVKTPRAKKKS"
     gene            complement(6665..7096)
                     /locus_tag="PK589_10560"
     CDS             complement(6665..7096)
                     /locus_tag="PK589_10560"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNJ20087.1"
                     /translation="MDLRKTFPRSMKTRLAGYVHLARMIDKCRAVLAGTEGEYIYPCP
                     MDERLLEYAGITAEQFTAAVKTNVTDDGVAEWFRRTATPHRAADLEDWNGKLLGRGPS
                     SPESAAKFAKYRDAIDPSRTDITAWSDLQDLEEGRPVPRRG"
     gene            complement(7096..7773)
                     /locus_tag="PK589_10565"
     CDS             complement(7096..7773)
                     /locus_tag="PK589_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 domain-containing protein"
                     /protein_id="HNJ20088.1"
                     /translation="MEHVAAVDTQPTVADDPQARAILQRAFENTARWQPDFRGFSADL
                     SVNTNGQVVSGTVVVKGPREVSVQLPDEAIQKWAQEQIGMIAVHRAPRKFEESDGKHR
                     LTMESGEGHPLGRRLDIHGDGMQSFYRIKDNRITQINRKMPHVAFTINVEESSTTQDQ
                     KQLTTKYTVYYFAPKDSTLRNVESFTDTHVRIGASDLPAARRIISYENGAVLVRTLTF
                     TNHKLLA"
     gene            8017..8364
                     /locus_tag="PK589_10570"
     CDS             8017..8364
                     /locus_tag="PK589_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20089.1"
                     /translation="MERRAASHTEEEEMNQRRRLLGLARKGDPKAISKLFELYQVRVL
                     NGDMLSKLNKSYYKAAAAQEQKAAQTPAKSAKGSQATHGSAKGSAKKSAAAAAPATKA
                     KPTKAATPKKRAK"
     gene            complement(8419..8712)
                     /locus_tag="PK589_10575"
     CDS             complement(8419..8712)
                     /locus_tag="PK589_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNJ20090.1"
                     /translation="MSTLTPVMTPDALDTTDVGTGDDLEARVIVFNCECHTYQQVIAL
                     FCKCIPGMNSSRAFELAWRIDHEGQATVYSGARKVAEEIGTKLAAGGLRVGVQ"
     gene            complement(8840..9532)
                     /locus_tag="PK589_10580"
     CDS             complement(8840..9532)
                     /locus_tag="PK589_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20091.1"
                     /translation="MTSLPRRHALFYPFHLCAPGTLEYLLEHYGSLHFRDYMALRLTP
                     FMGTTAYQDRMGDEHPDLVSNGRIVQGYAVSGPLDDTAVAEIDRDFSDPAWRGLFHAG
                     LQADRRFQRGLFDLTHAMRIGSTLVPGPAALLRLLEPQRATEVCTVGLVKHLARSSAT
                     LEDAYQFEYGLALVKTAAAQVYTFRLARAHGLVPVTDSKTHYRLLSHTMAREGIDLPN
                     EYVVPSQSPAAP"
     gene            complement(9618..9815)
                     /locus_tag="PK589_10585"
     CDS             complement(9618..9815)
                     /locus_tag="PK589_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20092.1"
                     /translation="MPVNMDPSKRRRRQPVESTASAADSETTQPKRFGEDTEADREKA
                     IAEAELKNLWDATEVIDMDSW"
     gene            complement(9889..11040)
                     /gene="hemW"
                     /locus_tag="PK589_10590"
     CDS             complement(9889..11040)
                     /gene="hemW"
                     /locus_tag="PK589_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNJ20093.1"
                     /translation="MSQATAQGLYIHIPFCHQRCHFCAFYLEIHQARAAEEFVSALLT
                     EVRVYGESGVLRQDPLDSIYFGGGTPTTLSSQQLATLLATSAQVFGLAPDAEVTIEAH
                     PGSVSRDSLAHLREAGCTRLSFGAESMDQTELDRVGRPGSPSTTLHVMAMAREAGFTN
                     INLDLMYGLPGQTLESWATSLRQTIELAPEHLSCYALTIEEGTHLKSSIQRGLLPAPD
                     EELQNRMEDLAEEILGRAGYDRYEISNYCRPGCASRHNRLHWTGGHYLGVGPSAQSYI
                     GGRRFGNVSDLTAYKAALRNGRLPVSESDQLTQAGESCERLIFGLRLVDGISLTQTGA
                     LSFPELSREVDKLLADELLERKGDLVRLTTRGRRYADTVAVSLLTSLDE"
     gene            complement(11056..11310)
                     /locus_tag="PK589_10595"
     CDS             complement(11056..11310)
                     /locus_tag="PK589_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20094.1"
                     /translation="MWSEDKSFVFRISLEAQFPDDYDGPLDEQAWLREWELQIKPVLI
                     KNLFDTLREYPAWKSHVRNRGKSPTEEIEVAMIRDFSPTP"
     gene            11390..11617
                     /locus_tag="PK589_10600"
     CDS             11390..11617
                     /locus_tag="PK589_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ20095.1"
                     /translation="MPIFEYVCQECNHRFELIVQGQTVPACPSCKGTTLNKQFSAFGV
                     GATGGWPVVSNSGGGCGSCGDPRGPGACSMN"
     gene            11645..11965
                     /locus_tag="PK589_10605"
     CDS             11645..11965
                     /locus_tag="PK589_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20096.1"
                     /translation="MMNDQEEQLRRAIGTLLQSDPLIKLLQEVRLGRMKATDAGLRAV
                     VESWIGTYAQVLKSQSLNAELLARLDPVPRLQVLVDAGVLSWEHSATCELRDLFRGLL
                     VPAA"
     gene            11985..12650
                     /locus_tag="PK589_10610"
     CDS             11985..12650
                     /locus_tag="PK589_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20097.1"
                     /translation="MRTHLAMALLVLAWLMPATVSAEIPSPLRPLHTTLSAALATLPS
                     DSHLLLDREPIEQFLAALDGSPPDWPQIYGQGHHDPGHDERLFALNRERDARREGKEP
                     LQWLVTVVWLGEVSRFDEEEGGFRVALGPKFNRTAWGEVRFKHEDLPATLIASAGGAT
                     DDLKARMQRGERIDIDVVMCGRLIPEESLVYDFSHEVDGLGLIMPVVRIEAVAFVLAD
                     GSR"
     gene            complement(12689..13333)
                     /locus_tag="PK589_10615"
     CDS             complement(12689..13333)
                     /locus_tag="PK589_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ20098.1"
                     /translation="MSILIIDDFQEERDLLHAILHRAGFGPLLPVATAQEGLTLLGIG
                     KRGKPGNGIDLVLMDLEMPGINGLEACEKIRMEEQLQGLPIIIITAHTAEKDIQAAYT
                     AGATDYIRKPVIPVELLARAATALSLKQEIDARKLREQELLERTKELDHAFEQMTTLH
                     GTLHICAKCKRVKTDGSYWQRIEDYLRRLARFTVKEAVCESCMHQAYPHLKRIP"
     gene            complement(13361..14113)
                     /locus_tag="PK589_10620"
     CDS             complement(13361..14113)
                     /locus_tag="PK589_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ20099.1"
                     /translation="MASQLSRASDTGSEPICRVLLADDSRESQALIQLYLRGLPYQLD
                     VVGNGKEAVAMFQSQQFDLVLLDQHMPVMDGFTAARVMREWEASNERAPVPILTLTAD
                     SLVDVKTQAQAAGCTACLAKPIVKEQLLDAFRRFGARPSPKKPVVTQNNSPAGMAALI
                     DEEITRRRPLFLDHRRQDLKRMQEAIEQGDFDAIRIMGHRIKGLAGSYGFHDIGLAGA
                     QLEQAAREQDIAAMRRTIDRLTTLLTQARQAV"
     gene            complement(14279..15175)
                     /locus_tag="PK589_10625"
     CDS             complement(14279..15175)
                     /locus_tag="PK589_10625"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ20100.1"
                     /translation="MRRLKATPRWFLLCLLVMLSLYQAAWASLSSDDMVLVPAGEFTM
                     GASVDRGGLPDEQPIRLVYLGSFLIDRYEVTNAAYLQFVQATNYQAPANAADRLTLWE
                     QNRPLPGIEQHPVVNVSWLDAVAFCRWANKRLPTEAEWEKAARGTDGRTYPWGNDWSL
                     DKANSASYWAGKTVEFADSREWEAFWIKGLGAELSKAKGLKGEILTLPVGSFPGGASP
                     YGALDMAGNAAEWVQDWYNPNHYRSASLTNPQGPERGAIKAMRGGSWLKPAISLRTSD
                     RDWGTMDSRPSGTGFRCARDSY"
     gene            complement(15172..15924)
                     /locus_tag="PK589_10630"
     CDS             complement(15172..15924)
                     /locus_tag="PK589_10630"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNJ20101.1"
                     /translation="MAQKRLTRAQFLERLLSIMDRKHHWAWPIIMGPGITKAQLKLHY
                     QQEYLVYVRDFPVLLARVHGQNPPPDVRRMLAENIYEEDTGGLSFGRSHPELFNEMMR
                     GLGFDSRVFLRATLLPASLRYRRWLEKVTASRDWVVGAAALAVFVEGSIKDRQEIQEP
                     SKPKTEAQIEAYIDAHPLIRHHKIEREHMDLIRAHQKVEAGHRQDAYTMVVNYAETRS
                     QQNAVLACVRQGLTLWAAYRDSIAKACKLTKP"
     gene            15972..16256
                     /locus_tag="PK589_10635"
     CDS             15972..16256
                     /locus_tag="PK589_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20102.1"
                     /translation="MKPITIQDSDEILNFLAEVALHGKGFTTDCLLDYVLEEGFTEPI
                     YLNASGEDPDAFYKNQPQAWAIYQVREWKRVLTVSGGPGKERRVQITETP"
     gene            16520..16750
                     /locus_tag="PK589_10640"
     CDS             16520..16750
                     /locus_tag="PK589_10640"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNJ20103.1"
                     /translation="MSLCEPADVGELALIRSLLDGNGIPYAIQHEHIGALYPGVALLG
                     SRVMVSECDRQRAEILLSRLALQVREATSETD"
     gene            complement(16796..17248)
                     /locus_tag="PK589_10645"
     CDS             complement(16796..17248)
                     /locus_tag="PK589_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20104.1"
                     /translation="MTDQTTSADPVSSLPPTAEPTPFIRQRPVRIIINSFLALFVVAL
                     IAFRFVPGWLDPDFSKHIESHRVMVGMDRKQVLDSWGSPNTMNVTHTSDGLRREEWIF
                     EDWESPAVVKHRYLYFEEGKLIGGHFSGSDVRLPLPTTPEPVKPKGHP"
     gene            17417..>18030
                     /locus_tag="PK589_10650"
     CDS             17417..>18030
                     /locus_tag="PK589_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20105.1"
                     /translation="MGRGGWRRDRVWAWVTALGLCLLGYAGVDAEAGSLYVYTDAQGQ
                     AVLTDNLEQIPAAYRGRVRTMTGPDSSAPAPGPAPAIAGNKTSTSTSSGVIGDILSAV
                     AAKVGARTIKGLTAYQTAVVIVAGASSLILLLFIFLSANPAIRILCKVLLVVVGLSAL
                     YQVLAIDAPSLEALAGPPQQGAGQPVDNMLGRMRSQTEQSYRAQG"
     assembly_gap    18031..18130
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18243..19022)
                     /locus_tag="PK589_10655"
     CDS             complement(18243..19022)
                     /locus_tag="PK589_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20106.1"
                     /translation="MAHQTVTRLIICCLLFFSLLGPECPSLWAGPYFEDGYLGLTQTE
                     LHAKLGLPQAVRDRKSALRVFTYYPISDWEQYFKKVVSPENGEDVYTFKRDGIDVRYS
                     FSFTFDPNDHNEQRPLFVRLVDIEFSPPVALSRIPQLVPEFRPTEDASAPVFRSNIML
                     LFFSGSPSADARFIVREKGKDALDWSLAFQMFALQGLPDPLTSKALIDRLEISTQSLP
                     LVKLRQRNTHEPVLNPYSKAFSQQPPPAPAVKKIPVPTYAE"
     gene            19209..22892
                     /gene="metH"
                     /locus_tag="PK589_10660"
     CDS             19209..22892
                     /gene="metH"
                     /locus_tag="PK589_10660"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNJ20107.1"
                     /translation="MPVHDIRELLRERILILDGAMGTMIQRHKLDEAAFRGERFKDWK
                     KDLKGHNDLLNVTQPSVIEAIHRQYLEAGADIVETNTFNSQSVSLADYGMEDLGYELS
                     KAGAECAKRAVAQVVKAQPARRCFVAGAIGPTTKTSSVSTDSNDAAARGTTYPELVQA
                     YGEQVRGLLDGGVDLLLVETIFDTLNAKAAFFAIQQLFAEGARQVPIMASVTFIQAGS
                     NRGFSGQTVEGFWNSISHVPLLSVGMNCALGPKEMRPLIEELSQLAPIFVSSHPNAGL
                     PNPLLPTGFPETPESLAPQLREWAQNGWLNIVGGCCGTTPDHIHTIAEAVRGVSPRRP
                     AKVEPFLRLSGLEALTVRPESNFVNVGERTNITGSPAFSKLILAGDYDKALTVARQQV
                     EGGAQIIDINMDEGLLDSKVAMQKFLRLLAAESDIARVPIMVDSSKWEVIEEGLRNIQ
                     GKGIVNSISLKEGETKFLEQARLIRRYGAAIVVMAFDERGQADSVARRIEICERSYRL
                     LTEQVGVPPQDIIFDPNILTVATGLEEHNNYAVDFIEATRWIKAHLPLAKVSGGVSNI
                     SFSFRGNNVVREAMHAAFLYHAIQAGLDMGIVNAGQLAVYEEIPKDLLALVEDVLLNR
                     RPDATERLVTFADTVKQKGKAAVKDDEWRQRPVEERLAHALVKGLTDYIDQDVEEARQ
                     KSARPLDVIEGALMAGMNIVGDLFGSGKMFLPQVVKSARVMKKAVAYLMPFMEAEKQR
                     LGTSRANGKVLLATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMVSCEKILETAREQQ
                     VDMVGLSGLITPSLDEMVHVAKEMTRQGFTVPLLIGGATTSKAHTAVKIAPSYANTTV
                     HVLDASRAVGVVGSLINKQQEQEFSASIRADYDRIRQAHQDRGSKALRTVEAARTHRM
                     PTDWASADIPTPSFTGLRQIDRMPLRELVPFIDWTPFFHTWELRGRYPEILEDATVGP
                     KAKELLADAQALLEEIIRGELLTARGVYGLFPANSVGDDIELYRNGDRRDVLTTFHTL
                     RQQTEKPADQFNLALADYVAPKDSGRGDYVGAFVVTAGIGVEALCAKYEKDHDDYNSI
                     MVKALADRLAEAFAEWLHKQVRREWGYGKAETLTNEDMIRERYRGIRPAPGYPACPDH
                     TEKRILFDLLQAESQSGVTLTESYAMLPAAAVSGLYFAHPGAKYFAVGKINRDQVEDY
                     AARKKLSVGEVERWLAPNLNYDA"
     gene            complement(23001..23498)
                     /locus_tag="PK589_10665"
     CDS             complement(23001..23498)
                     /locus_tag="PK589_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20108.1"
                     /translation="MKRTSRNWIAYLCENLASSGMMPTWEAATYLTDNLFGDKPNPRL
                     LRTTCPYEDTTHLLQRLYQPLVAAATRDMPLPAAAMIHVFTDISLTGKSAVLVVYFPG
                     PTQPHQLLLLDAAKAWELVFENSDEFNTWAEARYHSIKAALAKILSGVAPSPTLPASL
                     EAVKN"
     gene            complement(23495..24673)
                     /locus_tag="PK589_10670"
     CDS             complement(23495..24673)
                     /locus_tag="PK589_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ20109.1"
                     /translation="MSIPSVSEVTTQQSILENRHILIVDDEEPIRRLLGYLLEPHGYQ
                     VTLAGEAREARQRMEDGYYAMVLCDVNMPGESGMDLIRHILTQYPNTAVIMITGLDSP
                     VLANAALDMGAFGYIIKPFEANEVLINVANALRRRKLEIENAMHRENLEEVVRTRTIA
                     LQQALEWLERSEKELRLSREETIQRLAIAAEYRDSSTAQHIQRMSHYCELLARRYGLS
                     PDRCDLIRTASPMHDIGKIGTPDHVLLKPGKFTPEEFKVITQHTEIGYRILAGSDSDL
                     LKVAALIAWTHHERYDGTGYPRGIKGEEIPLEGRIVAIADNFDALTTQRVYKPAFDFD
                     HAKELMLKERGKHFDPDLLDIFFESMADVKRIFDQFADPSWLSSSRHRAITHDQGEAV
                     "
     gene            25127..25870
                     /gene="rph"
                     /locus_tag="PK589_10675"
     CDS             25127..25870
                     /gene="rph"
                     /locus_tag="PK589_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNJ20110.1"
                     /translation="MTSGAGLGRIDGRRRDQIRPVKVTRNFTKHAEGSVLIEMGDTKV
                     ICTASVEEKVPPFLKGKGTGWVTAEYAMLPRATHDRSPRESVKGKQGGRTLEIQRLVG
                     RALRAVIDTGRLGERTIWIDCDVIQADGGTRTASITGSFIALADAITVLKKKDLLKVN
                     PLTDYLAAISIGKVGGQVMVDLAYEEDSHAEVDLNLVMTGAGQYVEVQGTAERTPFNK
                     KDMDEFLDLGWGAIRELVDLQKSLIGALS"
     gene            25873..26484
                     /locus_tag="PK589_10680"
     CDS             25873..26484
                     /locus_tag="PK589_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HNJ20111.1"
                     /translation="MQIVLATRNQHKKQELVALLGGLDITIRTLDDFPEAPEVVEDGE
                     TCEANAMKKAVEISRFTGLPAVADDTGLEVDALGGRPGAFAARYAGEHATYEDNCRKL
                     LQELSGIPPARRGARFVTVAAIAFPGGKTMSAKGLLEGVIAESVVGSNGFGYDPVFIL
                     PEYNQTLGQLPPEVKNRISHRARAFAQARTLLQQAMAEHSVGA"
     gene            26475..26551
                     /locus_tag="PK589_10685"
     tRNA            26475..26551
                     /locus_tag="PK589_10685"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26509..26511,aa:Pro,seq:tgg)
     gene            complement(26652..28037)
                     /locus_tag="PK589_10690"
     CDS             complement(26652..28037)
                     /locus_tag="PK589_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNJ20112.1"
                     /translation="MQDIDSNPSFTLTDVPGGLPWLGHLRSFKHQPLRTMSGWWRQQG
                     DAVRFRLGPKTIHLFSHPALAEEVLLSQAERFGKVYEQQRPTGLALVLGNGLVTSSGE
                     VWKRHRRIIQPVFHRSRMAAMADRMVQVGEQRLAGWATHGEQPVDIAAEMMQLTLEVI
                     SQTMFHTSVAQHMDQISHSLRVSLKYAFDSFHNPLHLPVWVPTARNREFSRALKFMDT
                     LIYELVAARRQSGAHYDDLLNLLLQARDEETGMGLTDQELRDEALTIFAAGHETTANA
                     LAWTWYLLATHPEVRTRFHTEVDLVLRGRTPTADDLQHLPYTRAIFDESLRLYPPVPA
                     IQRKSMTQTTLGGFTLPANAIVLVGLYNLHRHPAFWADPEQFLPERWLDGERPPSRCA
                     YLPFGVGPRACVGTHFATVEGPLLLALIGQRYDPQLAQDHVEPEVMVTLRPKNGIRMT
                     LQQRHQSVGSE"
     gene            complement(28232..29089)
                     /locus_tag="PK589_10695"
     CDS             complement(28232..29089)
                     /locus_tag="PK589_10695"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNJ20113.1"
                     /translation="MPRRTRAASPTRPARAKDAVLDYPGYALKSGILAPSTIRAVQQR
                     LNQVGCGPISVSGVFDRATQEAVRLLQARSVDGQGQSLKIDGVVGPLTWAALFGAHRV
                     PSIATTPISALMKATLSIAASQVGVMEDPSGSNRGPKVDEYLKAAGIDPSTGSYAWCA
                     AFVYWSFKQAATRLGTTNPAIRTAGVLDHWKKAGQAGVTRLVAGQILDDLSLLKPGLV
                     FVINTGGGHGHMGLVEDFRDDRLVTIEGNTNLPGDREGVGVFRRSGRTISDINQGFLS
                     YDSQSHASS"
     gene            complement(29357..29761)
                     /locus_tag="PK589_10700"
     CDS             complement(29357..29761)
                     /locus_tag="PK589_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNJ20114.1"
                     /translation="MAVRNVAIFILYTSSGQLLLQHRTPDAYRLPNYWAFFGGGIEPG
                     ESPSDALKREVIEELSYHVQDPHFLMTQLFREGEDENTKYVFLERYQGQPLELGEGQA
                     MGWFLPDETHDLKMIDHDRAIVERVRQYLRDR"
     gene            30408..30809
                     /locus_tag="PK589_10705"
     CDS             30408..30809
                     /locus_tag="PK589_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ20115.1"
                     /translation="MLTPQRPLKLLIIDDSPDFQLLIKTYLEDFGIVCLFATDVVQAT
                     STAVREQPNLILLDIGLPGGGGLLLLERLRANLRTAKVPVIVITAQTTDGLESKARSL
                     GAEEFLHKPVEKDVLIDTLRRVLSRPPTQQA"
     gene            31063..31653
                     /locus_tag="PK589_10710"
     CDS             31063..31653
                     /locus_tag="PK589_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20116.1"
                     /translation="MIRLARKPDGMFSDLLDSAITQIVNDYLTSANARDQEDWLCASM
                     PRAARFFTAPQAREQLLAMQKIYHDERLYEVTKYHWLLLYECLETFAQGFNEQPVGWL
                     AQKYGIEKIDFHRLVDLFFWDTSFLMDHLAKMSRQETRTMLIWPESVGLAAGFKTHPD
                     ELAFILCDEGVTEEFAAQKDVPAWSIGSKSYPLPHT"
     gene            complement(31780..32196)
                     /locus_tag="PK589_10715"
     CDS             complement(31780..32196)
                     /locus_tag="PK589_10715"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HNJ20117.1"
                     /translation="MFCLVYASSATRPFSPSQLTDLLASCHANNSRLGITGILLYKNG
                     NFMQALEGEEEAVLKLYAKIFQDERHSGVLTLLQQTIPARQFPDWSMGFRDLNSPEVR
                     SLPGYNEFLNMPLTGAEFSSDPTYCQRLLTIFKKMM"
     gene            32552..33265
                     /locus_tag="PK589_10720"
     CDS             32552..33265
                     /locus_tag="PK589_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNJ20118.1"
                     /translation="MMRPPRRQSKAALVFLFDVDETLLDDQRVLSDLRRHLRREVGRE
                     GDRRYWNILQRLHRALGYADYLGALQKYRQSYPHDPHVLTLSSFLINYPFAKRVFPQA
                     MRVITRCRRVGSVVILSDGDVVFQPHKIQQSGLAAAADDHVLVYVHKEEQIRNIEVCY
                     PADHYVVVDDNVRILNVIKKIWRERVTTVWVRPSSSGTGRNGIHAHAHPDLTIHSIGE
                     LNKEHLRAIKLLQKEQSHS"
     gene            complement(33323..33559)
                     /locus_tag="PK589_10725"
     CDS             complement(33323..33559)
                     /locus_tag="PK589_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20119.1"
                     /translation="MSKHKRRDKHRPGSMDEMIVNAIDQGLLRSAATAGQLVMEQVLK
                     TDPEAKSLLETLVKQRLKQVLRNLDESGKANGKN"
     gene            35714..35790
                     /locus_tag="PK589_10730"
     tRNA            35714..35790
                     /locus_tag="PK589_10730"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35748..35750,aa:Arg,seq:tct)
     gene            37795..40299
                     /locus_tag="PK589_10735"
     CDS             37795..40299
                     /locus_tag="PK589_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNJ20120.1"
                     /translation="MEYRLRRRDQEYRWLLDQGCPRLDPDGTFLGYIGSCVDITERRR
                     AEAQLVESEERFRTLADNMSQFAWMADASGWLFWYNRRWYEYTGTTYEEMKGWGWQKV
                     HHPDYLDHVMETWRKAHATGEPWEDTFPLRGKDGMYRWFLSRAVPIRDNSGSITRWFG
                     TNTDITELRNAHAALKQSEARYRALYDSIDEGFCIIEVMFDEHMGAVDFRFLETNPAF
                     ERQTGIQNARGQRMREIAPQHEMHWFDLFGTIALSGEPKRFEYPAVELHRWYEGYAYR
                     VGEEYERKVGIIFNDITVRKQAEEKLRQSEQRLRLALSAGQMGAWDIDLLTGSVTWDA
                     RQCDLFGMSKTDEPVDMNKWYELVHPDDVGPLRAATARAETTGSFACEFRIVQPHSAE
                     RWIAGVGATLKNETGQAVRMVGLNYDITERRKTEADLRTSAEELEHVVQARTQELVLS
                     QERLRSLAAQLNLAEQRERKRLAGELHDSLAQWLVLCQLYLGQVSRGSLTSDVGEKIR
                     QTEDLLNQALNYSRTLIAELSPRVLQDHGLVAGLTWLGEEMQRHGLAVRVVATKGVET
                     TLSEDSSVLLFQSVRELLMNALKHARCNEVVVRLHRDEGNLCIEVQDDGVGFIATSNA
                     DETLSSKFGLFSIQERMTALGGMLNVQSRPGYGTIARLLLPLTSSATGLQPVKGGATV
                     IQAHSDSAPPSLNVTAPIRTLLVDDHPMMRQGLRSLLTTFANIDVVGEASDGQEAVEL
                     AERLRPRLIIMDVNMPRMNGIEATAVIKSRLTDTSVIGLSVHASPETDRAMRAAGAAI
                     LLPKETAAKALYQAIQKVLTDGLNIPDGAMGDCIKA"
     gene            complement(40455..41558)
                     /gene="fbp"
                     /locus_tag="PK589_10740"
     CDS             complement(40455..41558)
                     /gene="fbp"
                     /locus_tag="PK589_10740"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF014057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HNJ20121.1"
                     /translation="MKITLSIIKADIGSIGGHICPSQQLLQTVRSYIAQHGSPLLIDH
                     YLSSTGDDIAILMSHTHGPGHEAIHKLAWDAFVAGTEVAKKQGLYGAGQDLLKDAFSG
                     NVHGMGPAVAEMEFDERPNEPFLFFAADKTDPGAFNLPLYLAFADPMNTPGLMLAPNM
                     ARGFRFVIMDVNHTDGDRIIELSAPKDLYEIAALLRDTERYVVESIWSLSSGEQAVAA
                     STSRLHNIAGKYTGKDDPVMLVRVQKDFPATGEVLAPYAIGPYVAGGMRGSHQMPLMP
                     VPLQSGISYFDGPPVITCAAFAMHAGRLTEPVDTFVHPFWDRVRDNVSEKAISMRRQG
                     FFGAAMLPMAELEYTGIMEKLKGLDASFKVRKA"
BASE COUNT         8957 a        11392 c        12501 g         9447 t
ORIGIN      
        1 agacggccgc tttcttgact ttcaccatgc ctagcttaca gtatgcgccg gtctggaggg
       61 tagggtatgt ggaagaaaaa tttcttattc cgtgcgcatg aagcggctcc gctcaaagaa
      121 tcggaaaacg agctgttcca tgacgacgct gaaccggcct tggacagcgc cggcctacaa
      181 atggaaaagt ttctctcggt gtgggtgcag ggagaaggcg aagacgaaaa gcccaccatg
      241 tatacgaaca tgtatgtgcg tacggctacg ctcgatttta aaacccgggc gggatttctc
      301 cagcctctgc agggacgcac acatcagatc aagcagatgt tgacgccgga gcagaaggga
      361 tttctacgcg agtggttgtc cacgacctca cctcaagcct gggaagactc cgacgaccat
      421 tttcgaaccc tctttgatct cgagtgatcc attcaatcag gatgctgaag acctccgcca
      481 gcggcgttcc ctcatcattc agatcctcaa cgtaccgcaa gggtacgctc cggatcttca
      541 ttcgctgcgg ctttgctgga cgaacgtttt gaacctcctg tggaaatctt tctatggttc
      601 atatgcaagg gatcacagtg cgcgagtgtg taccgtctct cttcatccag ctagaccacg
      661 catgagtcat cccacatctc ttcctctgat ggccctgatt gtggccgggt tcatgtttct
      721 gtcgcaggga ccggtctgtg cctccgccac gattgaggtc tactatgctc ccgaagatca
      781 ccccattgat catgtggtga atctgtatgg ccgggcgacc agatacattt acgtctcggt
      841 gtacgggctg acggctccct ctatcgtgaa agcactggtg gaggcgaaac atcgcggcgt
      901 cgatgtgcga gtgattaccg atcgagaacg tctcactgat ccgaagcagc atagcgccgt
      961 gaagacattg cgactcgcgg gcgtgcccgt ccggatcaac cggcatgatg ccttgatgca
     1021 tctcaagcag gtggtgatcg acgacgagat caatacctcc gggtcggcga accacacgac
     1081 gagcggcaac cggtacaatg atgagcgact cgatgtcatc accgatgccc ggttgacggc
     1141 cagagcgcgc gagaaatttt tggccatgtg gaatgaccgc gagcgattcg tcgtctggac
     1201 agagtaagga gtggcatggc acgtgccagc atagagacag atgtagcgat tgtcggcgcg
     1261 ggcggcggag gcgccgtgtt ggcattgatg ctcgcgcaac agggggtacg ctccgtggtg
     1321 ttggaacggg cttctggtcc gccgcaaggt ctgcgcgggg aaattctgca gcccaatggc
     1381 cagcgggtgc tcgatcgtct agggttgctc gacaagctgc cggcgcatgc cgtgcgatcg
     1441 gtgcatcgat tcaacttctg tcgatccggc ggcgaacggc tgtgcacggt cgattacagt
     1501 gaccttccgg ctccttacaa tcgcgctgtg gtgacgctgc ccaatgtcgc gcatcatgcg
     1561 atcctcgatg cgttggaaaa gcagaatccc ggcgggctct ggtacgatgc cacctttacc
     1621 gggcttcgtt tcgacggcag ccgtgtcgtc ggtctgcagg cgacccgtca tggggagccg
     1681 gttgaggtgt cttcgcgttt ggtagtcggg gccgacggtg cattctccaa ggttcgcgag
     1741 tcactcggta tctcggcgga cattcatcgg tatccggaaa gttatctcat tgccatactc
     1801 aaagctccga cggggttcga tgaggcacgg tatctggtgg gcaaacgtga aattctggga
     1861 ctatttcccg ctgccggcca acaggtctac gcattctata tgatcaaggc cggatcgtat
     1921 gacgccgtga aggctcaagg acttgagtcg ttacgacggg cttgggtccg gatcgaccct
     1981 gccatggaat ccatcgtctc aggtttggtc gattggaatc agaccggcta tatgccgacc
     2041 gggcgtgttc gaaccgatcg gtgggtggca gatggcgcgg tgctcatcgg cgatgcggct
     2101 catgccatga atccccatgc atctcaaggc cgcatgcagg cgatggtgga tgcggtgacg
     2161 gtggccgatc tcattcccgg ctggctcaag aacaatacgt tttcgagtga agcgttgcgc
     2221 gccttcgaag ttgcccggcg gccgcaggtg agcatgttgc aacgtctggc agacgagcag
     2281 tgtcgattct ggaacaccgg gaacccgctg cttgccttcc tgcgcgatcg agtgtttcgt
     2341 acgctcgacc gtaatgcgcg cctccggtat cgtgtgctga cgaccaccgc cggactgcga
     2401 agccagcctc cgttttcgat gctcgatcgc gtgatggctg cggggttgtt gccagacccc
     2461 tctgcggatc ggaaatcatc ggtcggaaca tagaagaaga tggtccttgt aggccacgag
     2521 agtttcatca gagtaagaat gaattgtccc ggggcatgtt caaaaagtcg tccagcaagg
     2581 ccgcagccaa gcgaagagct gaggcgtacc cttgtggtac gttgaggctc cgaacgaggc
     2641 gagagcgcca ctggaagact tttcagcatc ctacccaact acagccgagg tgtttgtgac
     2701 tcaaccgacc gcgctcgatg tcccctccga ggtgcaacaa ctcctgcagc gatacgtcaa
     2761 ggaaacgacc actctgctcg gtccgcaact ggaggggatt ctcctgtacg ggagcgcggt
     2821 aggcggggag tttttgccgg gtcgttcaaa tctcaatctt ttgctgatgc tttccggcta
     2881 tgaccgggaa gggatgaaac ggtacgccaa agcccataag cgctggagcc gtgaacaatt
     2941 cgtcgtgccg ctcctggtga cggagaccga attgaccaaa cccggcaccg ttttcccact
     3001 cgaatatctg gaaatacagg agcaacatcg cgtgctctgg gggaaggatc cgtttatcgg
     3061 cctccatatc gatcgcacac atttagcgta tcaggtacag cagggtattc agggcaatct
     3121 ggtgcgattg cggcagcggt ttatcgaggg cggcgggacg gaggaagcga cgacgatgtt
     3181 attgccgctg tcgttgacgg ccttattgcc ctgtttgcgc gggttgcagc gcctggccgg
     3241 acacccggcg ttatttcaat cggacgcgtt gctcacggcc attcgccaca tgacgggact
     3301 cgatttagca ggactcggtg atgtgttgga attgaagcgc gggatcattt cgcccggacc
     3361 ggtggaagtc ccgcgcttat acgagcgcta tgccggaaat ctcgaagcct tgatcgcctt
     3421 gatcaggccg gatggcacgg tggcgccgcg atgaagaagc gctcactcac gatcaacgga
     3481 ctcatcccgg cgctctggct gctgtgtgta gtgatggcgg caccgttggg ccatgcgcgg
     3541 gaaccggttc cgaactatga tccccaaggg tatgtcagtg accatgcagg cgttatcgaa
     3601 gcggactggc gcgccagaat tcggtccgtc tgccaggacc ttgaacgaaa gaccggcgtg
     3661 gaaatggtgg tggtgactgt cccgacgctg aagccctatc cctcagccaa cgattatgcg
     3721 atgggcctct atcaaaaatg gggcatcggc tccgcccagg atgaacatgg ggtgttgatc
     3781 ctgttggccg tgcaggaacg ccaggcggcc gtgaccatgg ggcggcgcat gatcccggtg
     3841 atgggcgggg aggtggtcgg caaggtcgga catgagtatc tcgaccccgc gattaagaac
     3901 gggcatttcg acgaagggct gtatcgcacg gtagtcgcct tggcctctgt ctcgcaggag
     3961 atccgcgtcg gctccacgaa gaaggcgcat tttcgcggac tgggcttctg gatcaccgtc
     4021 accacggcga gcggagccct ctggtttttg tggtggctga gccgcccgga tttgcggcac
     4081 ccctttggcc gcatccgaaa gggacaatac tgggggaccg gacagggtgg attcggcggc
     4141 aacttcggcg gcatgggtgg cggaatgagc ggggagggat ggaagtaggg aggagggtgg
     4201 cccgttggtc tcccttgctc gcgcaacgcg cggtcgaaga ctcccctcgt ccgacgcgcg
     4261 cagtgggagc ccaatccagt ccacctcagg cttgatgcga catgaacgaa gagacggctg
     4321 gctgtcgctt tatccctgca gttgcgtcat catggtggct ggattccagt agccccacat
     4381 ggtttggatt tttccttcct gattaatctc gaacacatcg ataccctcga agaaaaccgg
     4441 ccgaccgttt ttgccggtac cccgtcccgt gaatttaacg gcgactctgt tcgcagactg
     4501 gaatacgtgg tcagctgtca tcctgatggt ttcaaaggca ccgagcaccc cgactagaaa
     4561 ttgtcgcaac gccgcacgtc cctttagcgg agccggcgct cctggttcat aactgatacc
     4621 ttcctcggcg aagcagctga cccacgaatc tgcatccatc ttcccaatgg cctcaaagta
     4681 ttgccgcacc gccgcaggaa tcgtctcgtc cttcatcgtt ttttctcctc tccctcatag
     4741 gcccggtcat atccggcgaa ttttcaagcg ctgttcatac tacgaaggaa ttcgcccgtt
     4801 ggattgaaat cgatgagtgc aaatggacgg ggtgtgacga acgacaaggc gggaaccagt
     4861 tggttccgcc acctggattg tggcgtggct cgcttggcgg tctgagccta ttccatgacc
     4921 cagagtgcga tgccggagag ttcccgagag agacgctctg cgacgttgtt actgaagagg
     4981 tgcgcggcat ccggttggct gtgcttcgtg atcacgaggg tgccatagcc gcccgctcgt
     5041 acttcatcga gaatggtgtc gacgagatca cctgcgtatc ccaatttgag catcacccgc
     5101 tcggtgggga agcctgtgtc cttgagtcgc tcgagcgctc tctcaaggat gggatattca
     5161 agggtctcct ccatctgcat ccattcttgc tgatctttgc gcagttgttc gccgagctga
     5221 tgttccatgt caggattttc cgatcccccg tgttccatca actcccgcgg catggggttc
     5281 aacacatgaa acaaggtgat ggccacggac ggcgtctcgc ggagcaatgc tccgacgtaa
     5341 tggaggacac ggatggtggc gtttgaatcg tcgacggcga tgaggatgtt tttgggagag
     5401 acggagcggt tcgaagtgtg gcccggggcg aagcgggatt ggtcatgcgg cgcagttgtg
     5461 gtcatgatgt caccatacct gggttggtgc gtgccggtcc tgtggctgtg accatctttg
     5521 agggagaggc tggttctgtg gcccgaagag gcttcatggt gccctcagag agacgggctg
     5581 gagcgcgaac cgtacggcac ctgctgggga tgcacagggt ccgcataggc ataagcctat
     5641 gcttctcctg ccaacaaatc aatcacgagc gcggagtgag agaaagcggg agatccgcgc
     5701 tcctgcgtga tcgcgtgcgt cccatgaagc cggggaattg gcttcatggg acgggaacga
     5761 gggatggaat caggacttct tcttggctcg aggtgttttg acagtcttgg ccgattttcc
     5821 acgggcaacg acaccgacgg gcatgacgcg attgcgtatc aggtatgacg cgatttcagg
     5881 cgcccggttg ccggcaggtc ctgccggatt gctgtccgca taggagctcg gaggattatg
     5941 tttgaacagc cagagttgta cgacgctcat gggcgtcatc ggtgcgggga taccggttcc
     6001 acgaaacttc atcatcccct gcgtttgaac gaggactgat ccgctggcga ccgacggata
     6061 caacgcgacg ccgcccatga gttcaatgag ggtttccatc gcgacatgat tgctctgcgg
     6121 aaagggcaca acttgcgtga ggccaaattt cgtaatgccg gcatgctggt gaagcaagct
     6181 gatttcttgt cccgtcgcat agcgaaagcc gtaggtggtg gtgtacccac cagcgccgcc
     6241 caagacgtcc atataggaca ggtttgccgt cgcgttcaga ctgagccatt cgagattcgt
     6301 ctcagtgtcg agtgtgatca agtgatcgcc ggtgccggga accaggttct gttgaacgag
     6361 agccatctac gtacctcctg gccgatggtt ggaatgatgg ggtatcgcga ttcacgcatg
     6421 tcttgtgcca ttcgcgtgcc agaatggatt cgtgcgaatg cgccctgaac agaccctgga
     6481 atggtgatgc cagtacgaag tggcacgtat agccgtgtac tcgcctcaca tacagaaccg
     6541 gagcgtatca tgagggatac agtgaggaat ctattcggat acgattagga gttcggtgcc
     6601 aaagaaggca agggtatggg aggagatcaa tccatgcgtg gtgatcgatt gactgatcaa
     6661 ggcactatcc tctgcgagga acgggcctgc cttcctccaa gtcttgaaga tcagaccagg
     6721 ccgtaatgtc agtccgcgaa ggatctatcg cgtcgcggta cttggcaaat ttcgcagcac
     6781 tttccggcga actcggaccg cgcccaagca acttcccgtt ccagtcctca agatccgctg
     6841 ctcgatgcgg agtggcggtg cgtcgaaacc actccgccac cccgtcatcg gtcacattgg
     6901 tcttcacggc agccgtgaac tgctcggccg taatgcccgc atattccagc agccgttcat
     6961 ccatcgggca gggataaatg tactcccctt cagtgccggc cagcaccgcg cggcacttgt
     7021 cgatcatccg cgccaggtgg acatagccag ccagcctggt tttcatgcta cgggggaatg
     7081 tcttccgcaa atccatcagg ccagcaattt gtggttggtg aatgtcaggg ttcggacaag
     7141 gaccgcgccg ttttcatacg aaatgatgcg ccgtgccgct ggaagatcag acgcgccgat
     7201 gcgcacatgt gtatccgtaa agctttcgac attgcgcaat gtgctgtcct tcggtgcgaa
     7261 ataatatacg gtgtacttcg tggtcagttg cttctggtcc tgggtggtgc tgctctcttc
     7321 cacattgatg gtgaaggcca cgtgcggcat cttccggttg atctgggtaa tccggttatc
     7381 cttgatgcga tagaaggact gcatgccgtc gccgtgaatg tcgagccggc ggcccagcgg
     7441 atgtccctcg ccgctctcca tcgtcagccg gtgttttccg tctgattcct cgaacttgcg
     7501 gggtgcgcgg tgcacggcga tcatgccgat ctgctcctgc gcccatttct gaatcgcttc
     7561 atcggggagt tggacgctca cctcccgcgg tcccttgacc acgacggttc cgctcacgac
     7621 ctgcccattg gtgttgacgc tgaggtcggc cgaaaaacct cgaaaatccg gttgccaccg
     7681 ggccgtattc tcgaaggcgc gttgaagaat ggcacgtgcc tgaggatcat cggcaacggt
     7741 cggttgtgta tcaacagcgg cgacatgttc catcggaggc tcctttgtgt cgtggaaggg
     7801 ataaaaaata attatagaga cgttgatcga tcatctacaa ctgtggagga catgatcttg
     7861 cgcaagaatc ccgttttgcc ggccggcaaa atttctctca tttgaccaag gtttgtgtag
     7921 tactggctag gccgaatctg ttatactgcg gggcatttca tccggcaatt tgacgaatgt
     7981 gtgacagaag tttcggcatt ccttgaaagg agcgctatgg agcggcgagc ggcgtctcat
     8041 actgaagaag aagaaatgaa ccaacggcgg aggctgttgg gactggctcg caagggcgac
     8101 cccaaagcca tcagcaagtt gttcgagcta taccaagtgc gtgtgctgaa cggcgatatg
     8161 ctgagtaagc tcaataaatc ctactacaag gcggcggctg cgcaggagca aaaggccgca
     8221 cagactccgg ccaagtccgc aaagggctcg caagcgactc acggcagcgc caaagggagt
     8281 gccaagaagt ctgccgcggc ggctgcgccg gcaaccaaag cgaaacctac gaaagccgcc
     8341 acaccgaaaa aacgagcgaa gtaatttatc tgtgtcgaga gggtcggcga agaccgccgg
     8401 ccctcttctc gaatcatctt actgaacacc cacccgcaat cctccggccg caagtttggt
     8461 tccgatttct tcagccacct tccgggctcc tgaatagacc gtggcctggc cttcatggtc
     8521 gatgcgccat gccaattcga aggcccggga agagttcatt cctggaatac acttgcagaa
     8581 cagggcaatg acctgctggt aggtgtggca ttcgcaatta aacacgatca cgcgggcttc
     8641 cagatcgtcg cccgtgccca cgtcggtggt gtcgagggcg tcgggggtca tgacgggggt
     8701 gagggtactc atgcggtcgt ctcgttcggc gccgagtgta ccaggtgaca cgggggcgag
     8761 tgcaacaggt tgtcagcctc tcttgacctt cgctgccgca gacgtcggac aaatatctta
     8821 cttggatagc gtgacgtcgt catggggctg cggggctctg ggatggcacg acatactcgt
     8881 taggcagatc gatgccctcc cgggccatgg tgtggctcag taaccgatag tgggtcttgg
     8941 aatccgtcac cggaaccaac ccatgcgcgc gggccaggcg aaaggtgtag acctgcgcgg
     9001 cggcggtttt cacgagcgcg agaccatatt caaattggta ggcgtcctcc agcgttgcgg
     9061 acgaacgtgc caagtgtttc accagcccca cggtacacac ttcggtagcg cgttgcggtt
     9121 cgagaagccg aagcagggcg gccggccccg gcacgagagt tgatccaatc cgcatggcat
     9181 gggtcagatc aaacaggcca cgttgaaacc gtcgatctgc ctgcaatccg gcatggaata
     9241 gccctctcca agcaggatca gaaaaatcac gatcgatctc ggctacggcg gtgtcatcca
     9301 gggggccgct gaccgcatag ccctgtacga tgcgaccgtt gctgacgagg tccggatgct
     9361 cgtctcccat gcgatcctga taggctgtgg ttcccatgaa tggtgtaagc cggagcgcca
     9421 tgtagtcgcg aaagtggagc gatccatagt gctcgagcag gtattccagc gtgcccgggg
     9481 cgcagagatg aaacggataa aacagggcgt gacgacgggg caaggaagtc atacgatggg
     9541 cttatccagt gactgggata tcgactcgga gagaggcctg caaatgaaga tcgaagcgat
     9601 gatttgtgcg aagaaggtta ccagctatcc atatcgatga cttcggtcgc gtcccacaaa
     9661 tttttcaact cagcttccgc gatcgctttt tcgcgatccg cttccgtgtc ctcaccgaat
     9721 cgtttgggct gagtcgtctc tgaatcggcg gcggatgcag tggattcaac cggctggcga
     9781 cggcggcgtt tactgggatc catattgacg ggcatgggaa gaagtctcct ttttcatttc
     9841 agtctcccct gtgtcgggga gggaagtcaa tgggataggc gaggctcact actcatccag
     9901 gctcgtcaga agagacaccg ccaccgtatc ggcataccgt ctgccccttg tggtgaggcg
     9961 aaccaggtcg cccttgcgtt cgagtaactc gtccgccagc aatttgtcca cttcccgtga
    10021 gagctcagga aatgagaggg ctccggtctg tgtaagggaa atgccatcaa cgaggcgcaa
    10081 cccaaagatg agtcgttcgc agctttcgcc cgcctgcgtc agctgatcgg attccgacac
    10141 agggagacga ccattcctca gggctgcctt gtaggctgtc agatcgctca cattgccgaa
    10201 tcgccgacct ccaatatagg actgcgcact ggggccgacg ccaaggtaat gcccgccggt
    10261 ccagtgcagg cggttgtgtc tgctggcaca tccaggtcgg caatagttgg aaatttcgta
    10321 gcgatcgtaa ccggctcgtc ccaaaatttc ttcggcgagg tcctccattc ggttttgaag
    10381 ctcttcgtcg ggggcgggga ggaggccgcg ctgaatagat gacttgaggt gagtgccttc
    10441 ctcaatggtt agggcgtaac acgacaggtg ttcaggcgcc agctcgatag tctgccgtaa
    10501 cgaggtggcc caactctcca gcgtctggcc cgggagtccg tacatgagat cgagattgat
    10561 gttggtgaag ccggcttcgc gagccatggc catgacatgg agagtggtgc tgggagagcc
    10621 cgggcgtccg acgcgatcga gctccgtttg atccatcgat tcggctccaa agctcaggcg
    10681 tgtgcatccg gcttccctga ggtgcgccag gctgtcccgt gaaaccgatc cgggatgggc
    10741 ttcaatggtg acctcggcgt ccggagccag tccgaacact tgcgcacttg ttgcgaggag
    10801 tgtcgcgagt tgctgggagg agagcgttgt gggcgtcccg ccgccgaaat agatggaatc
    10861 cagtggatcc tgtctcaata cgccgctctc gccgtacaca cgcacttccg tcaacagcgc
    10921 cgagacaaat tcttccgcag cgcgcgcctg gtgaatttcc aggtaaaaag cgcagaaatg
    10981 acagcgctgg tgacagaagg gaatgtgaat gtagagaccc tgagcggtcg cctgggacat
    11041 agccggctcg tgtgcctatg gagttggtga aaagtcgcgg atcatggcga cttcaatttc
    11101 ttccgtcggc gatttccccc ggtttctgac atgcgatttc caggcggggt attcccgcag
    11161 cgtgtcaaac aggttcttga tcaggaccgg cttgatctgc agttcccact cacgcagcca
    11221 ggcctgctca tcgagtgggc cgtcgtagtc gtcggggaat tgcgcttcca ggctgatgcg
    11281 aaatacaaag gatttatctt ctgaccacat gtgtggactc ctgtcggctg tgattgcgaa
    11341 agtgaattgg ccttacttat aatatttcaa aatccaagga gtacagccca tgcccatctt
    11401 cgaatacgtc tgccaggaat gcaaccaccg cttcgagctc atcgtgcagg gacagacggt
    11461 tccggcctgt ccttcctgca aggggacgac gttgaataaa caattttccg ccttcggcgt
    11521 gggggcaaca ggcggatggc cggtcgtctc gaattccggc ggcggctgcg gctcctgcgg
    11581 agatccgcgg ggtcccggcg cctgctccat gaattagcac gacgcgacct cattcgagtt
    11641 attcatgatg aacgatcaag aagaacaatt gcgtcgcgcg atcggcacgc tgctccagtc
    11701 agacccgttg atcaaattgt tgcaggaagt ccggctgggg cggatgaaag cgacagacgc
    11761 cggattacgg gcggtcgttg aatcctggat cgggacctac gcgcaggtct tgaagtctca
    11821 atctctcaat gccgaactgc tggctcgact cgatccggtg ccccgccttc aagtgctggt
    11881 ggatgccggg gtattgtctt gggagcattc tgccacatgc gagctcaggg acttatttcg
    11941 cggcctgctg gtccctgctg cctgacgtga ggagtgcgct ccaggtgcgt acccaccttg
    12001 ccatggcctt gctggtgctg gcctggttga tgccggctac cgtttcggcc gaaataccct
    12061 ctccgctgcg tccgttacat acgaccctgt ccgcagcgct ggcgaccctt ccttctgaca
    12121 gccatctcct tctcgatcgt gaacccatcg aacagtttct cgcggcgctg gacgggtcgc
    12181 ctcccgactg gcctcagatc tacggtcagg ggcatcatga tcccggtcac gacgaacggc
    12241 tgtttgcttt aaaccgtgaa cgtgatgccc ggcgcgaagg caaggaaccc ttgcaatggc
    12301 tagtgaccgt ggtttggctt ggcgaagtat cacggtttga cgaagaagag gggggattcc
    12361 gtgtcgcgct ggggccgaaa tttaatcgga cggcatgggg cgaggtacgt ttcaaacatg
    12421 aagatcttcc ggccacactg atcgcctcgg cgggtggggc gacggatgat ctcaaggcca
    12481 gaatgcagcg gggcgagcgg atcgacattg acgtggtgat gtgcggcagg ctgattcctg
    12541 aggagtcact cgtctacgat ttttctcacg aggtcgacgg cctcggcctc atcatgccgg
    12601 ttgtgcgtat cgaagcggtg gcatttgtcc tggcggacgg ctcgcgataa tggctggaat
    12661 atcgtctggt agttgagacg tggaacggct agggtattcg ctttaggtgg ggataggcct
    12721 gatgcatgca ggattcacag acagcttcct tgaccgtgaa gcgcgccaga cggcgtaagt
    12781 agtcctcgat ccgttgccaa taggatccat ccgtcttcac acgcttacac ttggcgcaaa
    12841 tgtgaagagt gccatggagc gtggtcattt gctcgaaggc atggtcgagt tctttggtcc
    12901 gttcaagcag ttcctgttca cgtaatttcc gcgcatcaat ttcctgcttc aggctcaatg
    12961 ccgtggcggc tcgtgccagc aactcgaccg gaatcacagg cttgcggata tagtccgtgg
    13021 cgccggccgt atacgcggcc tgaatatctt tctccgccgt atgagcggta atgatgatga
    13081 tgggcaaacc ctgaagctgt tcctccatcc tgattttctc gcaggcctcc aatccattga
    13141 tcccgggcat ttcgaggtcc atgaggacga gatcgatccc gtttccgggc tttccacgtt
    13201 tgccgattcc taagagggtg agtccttctt gtgccgtcgc gaccggaagg agtgggccga
    13261 atccggcacg atgcaggata gcgtggagga ggtctcgttc ctcctgaaaa tcgtcgatga
    13321 taagaatgct catgctggca agctttctct acggcatcgc ttaaacggcc tgcctggctt
    13381 gagtaagaag cgtagtcagg cggtcgatcg ttcggcgcat agcggcgatg tcctgctccc
    13441 tcgctgcctg ttccagctgg gctcccgcca gcccgatgtc gtggaatccg tacgagccgg
    13501 ccagaccttt gatgcggtgt cccatgatcc ggatggcgtc gaagtctcct tgctcgatag
    13561 cctcctgcat gcgtttcagg tcctgccggc gatgatcgag gaagagggga cggcgtcgcg
    13621 taatttcttc gtcgatcaac gctgccatgc ccgcgggcga gttgttctgt gtcacgactg
    13681 gtttctttgg agaaggtctt gcgccaaacc tccggaatgc gtccaggagc tgttctttga
    13741 caatgggctt ggccaagcat gccgtgcacc cggctgcttg ggcctgtgtc ttcacgtcga
    13801 ccagtgagtc ggcggtcaag gtcagaatag ggactggcgc ccgttcattg gatgcttccc
    13861 actcccgcat cacgcgcgcg gcggtaaaac cgtccatgac cggcatgtgt tgatccagaa
    13921 ggacaagatc aaactgttgg ctctggaaca tcgcgaccgc ttcttttcca ttccctacca
    13981 cgtcaagctg ataggggagg ccccgaagat acagttggat caaagcctgt gattcccgcg
    14041 agtcatcggc cagcaagacc cggcatatcg gttcagagcc ggtgtctgag gcacgagaaa
    14101 gctgggatgc catagccgac gctcgacgga tcaacgtgat gcggacgtga cagtacttag
    14161 aagtagtata gcgggtgaag cctggacgac ctaggatctc ctgaaaaccc ggcaggtatg
    14221 aggaatcgat cgctcagaga cctcgggcag gggcgaggct ggagaaacag cccttcgatt
    14281 agtagctatc cctggcgcaa cggaatcccg tcccggaagg gcggctgtcc atcgtccccc
    14341 aatcacgatc ggaggtacgc aggctgattg ccggtttgag ccaggatccg ccgcgcatag
    14401 ctttaatcgc gccacgctct ggtccttggg gattcgtgag cgatgcggag cggtaatggt
    14461 tggggttata ccagtcctgc acccattcgg cggcattgcc tgccatgtcc agcgctccat
    14521 acgggctggc gccgccagga aaactcccga ccggcagcgt gaggatttcc cccttcagac
    14581 ccttcgcttt ggagagttct gcgcccaggc ctttgatcca gaacgcttcc cactccctgc
    14641 tatccgcaaa ctctacggtt ttcccggccc agtagctcgc actgttcgcc ttgtcgaggc
    14701 tccagtcgtt gccccagggg taggtccgtc cgtcggtccc ccgggcggcc ttttcccatt
    14761 ccgcttcggt tggcaaccgt ttgttcgccc agcggcagaa tgcgacggca tcaagccaac
    14821 tcacattcac gaccggatgc tgctcaatcc cgggaagtgg ccggttctgt tcccatagag
    14881 tcaagcgatc ggcggcattg gcgggggcct gatagtttgt ggcctggacg aattgcagat
    14941 aggcggcgtt cgtgacctcg taccgatcga tcaagaacga cccgagatag accaggcgta
    15001 tcggttgctc atccgggagg ccgccccggt caacggaagc tcccatcgtg aattcgccgg
    15061 cgggaaccag caccatgtcg tcggatgaaa gggatgccca ggctgcctga tacagactga
    15121 gcatgacgag caggcagagc agaaaccacc gaggggtagc cttcagcctc ctcatggctt
    15181 cgtcagtttg caggcttttg cgatgctgtc gcgataggcc gcccacagcg tgagcccttg
    15241 cctcacgcaa gccagcacgg cattctgctg gctcctcgtt tcggcatagt tgacgaccat
    15301 ggtgtacgcg tcctggcgat gccccgcctc aaccttctga tgcgcgcgga tgagatccat
    15361 gtgctcccgc tcgatcttgt gatgacgaat gagaggatgg gcgtcgatat aggcttcgat
    15421 ctgggcctcg gtttttggct tggagggttc ctgaatctcc tgacggtctt taatactgcc
    15481 ctcgacaaac accgcaaggg cggcggcgcc gaccacccag tcccggctgg ccgtgacctt
    15541 ttccagccac cgccgatagc ggaggctggc tggaaggagg gtggctcgca ggaagacccg
    15601 gctgtcgaac cccagcccgc gcatcatttc gttaaatagc tcgggatgag atcgcccgaa
    15661 ggacaggccc ccggtatctt cttcgtaaat gttctcggcc aacatgcgcc ggacgtcggg
    15721 gggcgggttc tgcccatgca ctcgagcgag caggacaggg aagtcccgca cgtagaccag
    15781 gtattcctgt tgatagtgga gctttagctg ggccttagtg atcccgggtc ccatgatgat
    15841 cggccaggcc caatggtgtt tgcgatccat gatggacagg agccgctcga ggaattgcgc
    15901 acgggtcaat ctcttctgtg ccatgccttg ccttccttcg ttcctactgg ctacaatcag
    15961 ccaccggtca gatgaagcct atcacgattc aggattccga cgagattctc aacttccttg
    16021 ccgaggttgc cctgcatgga aaagggttta ccacggactg tctgttggac tatgtgctgg
    16081 aagaagggtt tacggagccg atttacctca atgcctccgg tgaagacccg gacgcattct
    16141 ataagaatca gccgcaggcc tgggccattt accaagttcg tgaatggaaa cgtgttctga
    16201 ccgtgtcggg cgggcccggg aaggaacggc gcgttcagat taccgagacc ccgtaagttg
    16261 tccgttgtcc cccttcggca aggacatcaa cgccgatctc cgcgcagtca ttgctccata
    16321 tccaaggcca gcttctgctt ccggacgcgg tgtgcgcttc cgtgagtgta ttgaccatcc
    16381 gaaggaattg caacgaatca cgcgctgttc gcagtccggg gcgcctggtc ctgtccattc
    16441 cgtttggtaa atgtgcaagg gtgtctggcg agaggccatt gtgacattac ggcatcatcc
    16501 taggaatctt cagcctctgg tgagcctctg cgaaccggca gatgtgggtg aattggccct
    16561 gatccggagc ctgctcgacg gcaatggcat tccctacgcc attcagcatg aacacatcgg
    16621 cgcactctat cccggggtgg cgctcttggg cagccgcgtt atggtgtcgg aatgtgaccg
    16681 gcaacgggcg gagattctgt tgagccgcct ggccttgcag gtccgtgagg ccaccagcga
    16741 gacggattga aacgggatca ggtggtcctg gcccggagtg gtcgttaggt gaggcttacg
    16801 ggtggccttt gggtttgacc ggttctggag tggtgggcag gggcaggcgc acgtcggatc
    16861 ctgaaaaatg gccgccgatc aacttgccct cttcaaagta gagatagcgg tgcttgacga
    16921 ccgccgggct ttcccaatct tcaaagatcc attcttcgcg ccgtagccca tcgctggtat
    16981 gggtgacgtt catcgtgttc ggcgaccccc acgaatccag gacctgtttg cgatccatcc
    17041 ccaccatcac gcgatggctt tcgatatgtt tggagaaatc cgggtccagc cacccgggca
    17101 cgaagcgaaa cgcgatcaag gcgaccacga acagcgccag gaacgaatta atgatgatcc
    17161 tgaccggccg ctggcggata aacggtgttg gctccgctgt cgggggtagg gaggaaacgg
    17221 ggtctgccga agtcgtctga tcggtcacaa atatccttcc atcaacgagt gggcgagtgc
    17281 gaaatgcctg ctgcatgcta aacaataggt tttcggtccg tcaagggagg gaatcaccct
    17341 caagaaaggg catttcgccg cctgtacccc ggtttcgacc tgctatgctt aacggaggac
    17401 cgaaggaggg cgaaaaatgg ggcgtggcgg ctggaggcgg gaccgggtgt gggcgtgggt
    17461 gactgcactg ggcctttgtc tcctgggata tgcaggggtt gatgccgagg cgggctctct
    17521 ctacgtctat actgatgccc agggccaagc cgtcttgacc gacaatctcg agcagatacc
    17581 ggccgcctat cggggccggg tgcgtacgat gactggtccg gactcctcgg ctcctgctcc
    17641 tggacccgca ccagcaatcg ccgggaataa gacatctacg tctacgtcct ccggcgtgat
    17701 tggggatatc ctcagtgccg tggcggcaaa agttggcgcg cgaacgatca aagggctgac
    17761 ggcctatcaa acggccgtcg tgattgtggc aggcgcttct tccctgatcc tgctcttgtt
    17821 tatttttttg agtgccaatc ctgccatcag gattctttgc aaggtgctgc tggtagtggt
    17881 gggactgtcc gctctctacc aggtcctggc gatcgatgcg ccgtccctgg aggcgcttgc
    17941 gggacctcct caacaggggg caggtcagcc ggtggacaat atgttgggac ggatgcgaag
    18001 ccagaccgag cagagttacc gcgcacaggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18121 nnnnnnnnnn acaatatgtt gggacggatg cgaagccaga ccgagcagag ttaccgcgca
    18181 cagggtgaac gcacggccag ccagttggag ccagcagagc aaccagctcc ctgacacgcg
    18241 tgctactcgg cgtaagtggg taccggaatc tttttcacag caggcgcggg cggcggctgc
    18301 tgtgagaagg ccttggagta gggattcagg accggttcgt gggtgttgcg ctggcgcagt
    18361 ttgaccagag gcaggctttg ggtactgatt tccaggcggt cgatcagcgc ttttgaggtc
    18421 agcggatccg gcagaccttg taacgcgaac atctggaagg cgagtgacca gtcgagtgca
    18481 tcctttcctt tctcccgcac aatgaaccgc gcatcggccg acgggctccc cgaaaaaaac
    18541 aacaacatga tgttggagcg gaacaccggc gcggaggcgt cctccgttgg tctgaattca
    18601 ggaacgagtt gaggaatcct gctcaaggcc accggcggag agaattcgat atcgacgagg
    18661 cggacaaaca ggggccgctg ttcgttatga tcgttgggat caaacgtaaa gctgaaggaa
    18721 taccggacat cgatgccgtc ccgcttgaag gtgtagacat cttcgccgtt ttcaggggag
    18781 acgactttct tgaagtattg ctcccagtcg ctgatggggt aataggtgaa gacgcgcaag
    18841 gcggatttgc ggtcccgtac ggcctggggc agcccgagtt tggcgtgtaa ttcggtttga
    18901 gtcagcccga gatagccatc ctcaaaatac gggccggccc acagggaagg gcattccggt
    18961 ccgaggaggc tgaagaaaag aaggcaacaa ataatgaggc gggtgactgt ctgatgcgcc
    19021 atacaatcga ctcaggctcg gcccagacac tgtgtggcat cgtatcggcc ggggtagtca
    19081 ttgtcaaggc ggagagcccg tcatggcttg ctgaaaaaag tccgttcccg atatgatgcc
    19141 cgctcaatgg cagtcatcgc ccgagtcgcc gcacgcctga agtcggcgac agaaagacat
    19201 aggtagtcat gccggtacac gatattcgag agctgctccg tgaacgtatc ctgatcctcg
    19261 acggggccat ggggaccatg atccagcgtc ataaactgga cgaagccgcg tttcgcgggg
    19321 agcggttcaa ggattggaaa aaagacctca agggacacaa cgacctgttg aacgtcactc
    19381 aaccgagtgt gattgaggca atccatcggc agtatctcga agccggggcg gacattgtcg
    19441 aaaccaatac cttcaattct caatccgtgt cactggccga ctacggaatg gaagatcttg
    19501 gctatgagtt gtccaaagcc ggagcggaat gtgccaagcg ggcggtcgct caggtcgtga
    19561 aggcgcagcc tgcgcggcgc tgtttcgtgg cgggcgcgat cgggccgacc acaaagacct
    19621 cgtcagtgtc gacagactcc aatgacgcgg cagcacgcgg gaccacgtat ccggaattgg
    19681 tgcaggccta tggcgagcag gtacgcggtc tgctggacgg cggcgtcgac ctgctgctcg
    19741 tcgaaacgat cttcgacacg ctgaatgcga aggcggcttt ttttgcgatt cagcaactct
    19801 tcgcagaagg tgcccggcag gtcccgatca tggcgtcggt cacgtttatc caagccggga
    19861 gcaatcgcgg cttctcggga cagacggttg aagggttctg gaattctatt tcgcatgtgc
    19921 cgctgctgag tgtgggcatg aactgcgcgc tgggcccgaa ggaaatgcgg cccttgatcg
    19981 aagagctgtc gcagctcgcg ccgatttttg tcagcagcca tccgaatgcc ggcttgccca
    20041 atccgttgtt accgacgggc tttcccgaaa cccctgagtc gctggcccct caactgcgtg
    20101 aatgggcgca gaacggctgg ctcaatatcg tcgggggctg ctgcgggact acgcccgacc
    20161 atattcacac tattgcggaa gcggtgcgcg gggtgtcacc ccgtcggccg gccaaggtcg
    20221 agccgttttt acgcctcagt ggcctggaag ccctgaccgt gcggccggaa tcaaacttcg
    20281 tgaatgtcgg cgagcgtacc aacattacgg gatcacccgc tttttcgaaa ttgattctgg
    20341 ccggtgacta cgacaaggcc ctgaccgtgg cgcgccagca ggtggaaggc ggggcgcaga
    20401 ttatcgatat caacatggat gagggattgc tggactccaa ggtggcgatg cagaagttcc
    20461 tgcgtctcct ggccgccgag tcggacattg cgcgcgtgcc gatcatggtc gacagctcca
    20521 agtgggaggt catcgaagaa gggctccgca acattcaagg gaaaggcatc gtcaattcca
    20581 tcagtttgaa agaaggcgag accaagtttt tggagcaggc gcgcctcatc cggcgctatg
    20641 gcgcggcgat tgtcgtcatg gcgttcgacg agcgtggcca agccgactcg gtcgcgcggc
    20701 gcatcgagat ctgcgagcgc tcctatcgac tgctgacaga acaggtcggt gttccgccgc
    20761 aggacattat tttcgatccg aatatcctca cggtcgcgac cggattggaa gaacacaaca
    20821 attatgccgt cgatttcatc gaagcgacgc gctggatcaa ggcgcatctc ccgctggcca
    20881 aggtcagcgg cggcgtcagc aatatctcct tttcattccg cggcaacaac gtggtgcgcg
    20941 aggcgatgca tgccgcgttt ctctaccatg cgattcaggc cggcctggat atgggcatcg
    21001 tcaacgccgg tcagctggcc gtgtatgaag aaattccgaa ggacctgctc gcgctggtgg
    21061 aggacgtgct gttgaatcgc cgtcccgacg cgactgaacg gttggtgacg tttgcggaca
    21121 cggtcaagca aaagggcaaa gcggcggtta aagacgatga gtggcgccag cggccggttg
    21181 aggagcgttt ggcccatgcg ctggtgaagg gactgacgga ctacatcgat caggatgttg
    21241 aggaggcgcg tcagaaatcc gctcggccgc tcgatgtcat cgaaggggcg ctgatggccg
    21301 gcatgaatat cgtcggcgat cttttcggtt cggggaaaat gtttttgccg caagtggtga
    21361 aaagcgcccg ggtcatgaag aaagctgtcg cgtacctcat gcctttcatg gaggcagaaa
    21421 aacagcgatt gggcacctcc cgcgccaacg gcaaggtgtt actcgcgacc gtcaagggcg
    21481 acgtgcacga tatcggcaag aacattgtgg gagtggtatt gggctgcaac aactacgagg
    21541 tcatcgacct tggcgtcatg gtgtcctgcg aaaaaattct ggagaccgct cgtgaacagc
    21601 aggtggacat ggtgggcctg agcggattga tcacgccatc attggatgaa atggtgcatg
    21661 tggccaagga gatgacgcgc cagggattca cggtgccatt gctcattggc ggtgccacga
    21721 ccagtaaggc ccacacagct gtgaaaatcg cgccgtccta cgccaatacg acggtgcatg
    21781 tgctggacgc atcgcgcgcc gtcggggtgg tgggaagcct gatcaacaag cagcaggaac
    21841 aggaattttc tgcttccatt cgcgcggatt acgatcgcat ccgccaggcg catcaggacc
    21901 gagggagcaa ggcactccgc accgtggagg ccgcgcgaac ccaccggatg ccgaccgatt
    21961 gggcttcagc cgacattccc acgccctcgt tcaccggact gcggcagatc gatcgtatgc
    22021 cgttgcgcga actggtgccc ttcatcgact ggacgccgtt ctttcacacg tgggaattgc
    22081 gcggccggta tcccgagata ttggaagacg ccactgtcgg cccgaaggcc aaggaactgc
    22141 tggccgatgc ccaggcattg ctggaggaga tcatccgcgg cgaattactc acggcgcgtg
    22201 gagtctatgg attgttcccg gccaatagcg tcggcgatga tatcgaattg tatcggaatg
    22261 gcgatcgccg tgatgtcctc accacctttc atacactccg gcaacaaacg gagaaaccgg
    22321 ccgatcagtt caacctggcc ctggccgact atgttgcccc caaggattct ggccgtggcg
    22381 actacgtggg ggcttttgtg gtgacggcag gcatcggtgt ggaagcgctc tgtgcgaagt
    22441 atgagaagga ccacgatgac tacaactcca tcatggtgaa ggcgctggcc gatcgattgg
    22501 cggaagcgtt cgccgaatgg ctgcacaagc aggttcgccg tgaatgggga tacgggaaag
    22561 ccgagaccct gaccaacgag gatatgattc gggagcggta ccgcggcatt cgtccggcgc
    22621 cagggtaccc ggcttgtccc gaccacacgg aaaagcgaat cctgttcgat ctcctgcagg
    22681 ctgaatcgca gtcgggtgtg acgttgacgg agtcctatgc catgttaccg gcggcagctg
    22741 tcagcggtct ctactttgcg caccctgggg ccaagtattt tgccgtgggg aaaatcaacc
    22801 gcgatcaggt ggaagactat gcggcgcgga agaagctttc tgtgggcgag gtggagcggt
    22861 ggttggcgcc gaatctcaac tacgacgcat agcaggataa tggaagagtt gcagattcgt
    22921 tcttgcctca cgcacaggtt gaacctactc tcagcgtacg cctcggccca agacagggcc
    22981 ggctcactcg ccggtcatgg tcagtttttg acggcttcga gagatgcggg caaagtcggg
    23041 gagggggcta cgccgcttaa gatcttggca agcgccgcct tgatcgaatg gtaacgtgct
    23101 tccgcccagg tattgaactc gtctgaattc tcaaacacca gttcccaggc tttagcggca
    23161 tccagcaaca ggagctgatg cggctgagtg gggccgggga aatacactac caatacggcc
    23221 gattttcccg tgaggctgat atccgtgaac acgtggatca tggcggccgc tggcagcggc
    23281 atatcacgtg tggccgcggc gacgagcggc tggtacaacc gctggagcaa gtgcgtcgta
    23341 tcctcgtacg ggcacgtcgt gcgcaagagt cgcgggttgg gcttgtcccc gaacagatta
    23401 tcggtcaggt aggtggcggc ttcccacgtc ggcatcatgc cggaggaggc cagattctca
    23461 cacagatagg cgatccagtt tcgtgacgtc cgcttcatac ggcttccccc tggtcatgag
    23521 tgatggcacg atgccgggag gaggacagcc agctcggatc agcgaattga tcgaaaatcc
    23581 gtttgacgtc agccatggat tcgaaaaaga tatcgagcag gtcgggatca aaatgtttgc
    23641 cccgttcctt cagcatcagt tcctttgcgt ggtcgaaatc aaacgccggc ttgtacaccc
    23701 gctgcgtggt gagggcatcg aagttatccg cgattgcgac gatcctgcct tcgagtggaa
    23761 tctcctcccc cttgatcccg cgaggatagc ccgtgccgtc gtaccgctcg tgatgggtcc
    23821 aggcgatgag cgcggcgacc ttcagaaggt ctgagtccga cccggccaga atcctgtagc
    23881 cgatttctgt atgttgcgtg atgactttga attcctccgg cgtgaacttg ccgggcttga
    23941 gcaggacatg gtccggggtg ccgatcttgc cgatatcatg catggggctg gcggtgcgga
    24001 tgagatcgca tcgatcgggc gataatccgt accggcgtgc caacagctcg cagtagtggc
    24061 tcatccgttg gatatgctgt gcggtcgagc tgtcgcgata ttccgcggcg atggcgagtc
    24121 gttgaatagt ttcttcgcgg gagagccgca gttccttttc gctccgctcc agccattcca
    24181 atgcctgttg cagcgcaatc gtcctggtcc ggacgacctc ttcaagattt tcccgatgca
    24241 tggcattttc gatttccagc ttgcgacggc gtaacgcatt tgccacgttg atcaggactt
    24301 cgttcgcctc gaagggctta atgatgtatc cgaaggcgcc catatccagg gcggcattgg
    24361 ccaggaccgg gctgtcgagc ccggtgatca tgatgacggc cgtgttggga tattgcgtca
    24421 aaatgtggcg aatgagatcc atgcccgatt cgccaggcat gttcacatcg cacaacacca
    24481 tggcgtagta cccgtcctcc atccgttgac gggcttcgcg agcctcaccg gccagggtca
    24541 cctggtagcc atgcggctcg agcaaataac ctaacaggcg gcgaatgggc tcttcgtcgt
    24601 ccacaatcag gatatggcgg ttttcgagaa tggattgttg cgtggtcact tcactgacgc
    24661 tcgggatgct catacggctc actcgcgggt taaggtcaag tgctgctgaa tcagggatag
    24721 aatcagtatc ggtttttacc aactcattct gaagtcatgg tagctggttc ctgaattttg
    24781 caccttttgt gccaatacaa cctccctgcc gtgggctgca gaagtacatt gaatggccca
    24841 acaatgagca ggactgatcc agatccggac aggatcctag tcggcggtaa gggcgtggcc
    24901 gtcaagtctg acgccggtcg gtcggctgac gtcgggaatg tgtcgtttac ggacagccaa
    24961 tctatgcagc cattgtggtg aattatacag gggcacttgc tgaatgccag ttcaaacgca
    25021 gtttgagaaa tcggtggact cgttcggctg tcgggatttg ctctttgagg ggccgttcac
    25081 tataatccgc tcctttggat tgccgtgcag gaaggagcgc gactgaatga cgagcggggc
    25141 aggattgggg cgaattgatg ggcggcgacg ggatcaaatc cgccccgtca aagtgacgcg
    25201 caattttacg aagcatgctg aaggctccgt cctgatcgaa atgggcgaca ccaaggtgat
    25261 ttgcaccgcg tcggtcgagg agaaagtccc gccctttctc aaaggaaagg gcaccgggtg
    25321 ggtgacggcc gaatatgcca tgttgccacg ggccacccat gatcggtcgc cgcgtgaatc
    25381 ggtcaagggg aaacagggcg gccggacgct ggagattcaa cgcctggtcg gacgggctct
    25441 ccgcgcggtc atcgataccg gacggttggg tgaacgaact atctggatcg attgcgatgt
    25501 catccaggcc gatggcggca ctcgcaccgc ttcgatcacc ggatcattca tcgccttggc
    25561 ggatgccatc accgtgctga aaaagaagga tctgctcaag gtcaatccgt tgaccgacta
    25621 tttggcggcc atcagcatcg ggaaggtcgg tggtcaggtg atggtggatt tggcctacga
    25681 agaagattcc catgccgaag tcgatttgaa tctcgtcatg accggggcgg gacaatatgt
    25741 tgaagtgcag ggaacggccg aacgcacgcc cttcaacaaa aaagatatgg atgagtttct
    25801 ggatcttggt tggggcgcca tccgggaatt agtggatttg cagaagtcgc tgatcggcgc
    25861 cttgagctaa ccatgcagat cgtgctggcc acgagaaatc agcataagaa gcaggaactt
    25921 gtcgcgctac tgggcggctt ggacatcacc attcggaccc tcgatgattt tcccgaggct
    25981 ccggaagtgg tggaagacgg tgagacctgc gaagcgaatg ccatgaagaa ggccgtggag
    26041 atctcgcgtt tcacgggatt gccggcggtc gcggacgata cggggctgga ggtcgatgcg
    26101 ctgggcggtc ggccgggagc gttcgctgca cgatatgccg gcgagcatgc gacctacgaa
    26161 gacaattgca ggaagttatt gcaggagctc agtggcattc cgcctgcgcg acggggggct
    26221 cggtttgtga ccgtggccgc catcgcgttt cctggcggca agaccatgtc ggccaagggc
    26281 ctgttggaag gggtcattgc cgaatcggtc gtgggctcca acggattcgg gtatgatccg
    26341 gtctttattc tgccggagta taatcagacg ttgggacaat tgccgccgga ggtcaagaat
    26401 cgaatcagcc atcgggcccg cgcctttgcg caagcgagga cgttgctgca acaagcgatg
    26461 gcggagcact cagtcggggc gtagcgcagc tcggtagcgc gcctgctttg ggagcaggat
    26521 gtcggaggtt caaatcctct cgccccgacc aaattccttc cctcggtctg gattccttcc
    26581 gttttttccg attccaatct gcgtcaggct ccactgccac tcagtgagct cccgaggaac
    26641 ttccacccgc tttattcaga gccgacagac tgatgccgct gttgaagcgt catacggatg
    26701 ccgtttttcg gccgcaacgt caccatgact tccggctcga cgtggtcctg tgccagctga
    26761 ggatcatagc gttgaccgat cagcgccaaa agcagggggc cttctactgt cgcgaagtgc
    26821 gtgcccacgc aggcacgcgg tccgacgccg aagggcaggt aggcgcagcg ggaaggcgga
    26881 cgttctccgt ccagccaacg ttcaggcagg aactgttccg ggtctgccca aaaagccgga
    26941 tgccggtgca ggttatataa gccgacgagg acgatggcat tggccgggag ggtaaatccg
    27001 ccgagcgtgg tctgcgtcat gcttttgcgt tggatggccg gcaccggggg atagaggcgg
    27061 agcgattcat caaagatcgc acgtgtgtag ggcagatgtt gcagatcgtc ggcggtcggg
    27121 gttcgcccgc ggagaaccag gtccacctcc gtgtgaaacc gtgtcctcac ctcgggatgg
    27181 gtggcgagca aataccaggt ccacgcgagt gcgttagcgg tcgtttcatg tcccgccgca
    27241 aagatcgtca gggcttcatc acgaagttcc tgatcggtca gtcccatgcc ggtttcctca
    27301 tcccgagctt ggagcagcag gttcaggagg tcgtcatagt gcgcgccgct ctgtcgccgg
    27361 gccgcgacca attcgtagat cagcgtatcc atgaatttca gggctcgact gaactcacgg
    27421 ttgcgagcgg tcgggaccca gaccgggaga tgcagcgggt tatgaaatga gtcgaacgca
    27481 tatttcaaac tcacgcgaag agaatgactg atctgatcca tatgctgagc gacgctggta
    27541 tgaaacatcg tttgggaaat gacttcgagc gtcaactgca tcatttcagc ggcgatgtct
    27601 accggctgct cgccatgagt cgcccacccg gcgagccgtt gttctcccac ctgcaccatt
    27661 cgatccgcca tcgcggccat acgagagcga tgaaacaccg gttgaatgat gcgacggtgc
    27721 cgcttccaca cctccccgga actcgtcacc aatccattac ctagcactag cgcgagtccg
    27781 gtgggacgtt gctgttcgta cacttttcca aaccgctcgg cttgtgagag cagcacctct
    27841 tccgccaacg cgggatggct gaagagatga atggtcttgg gaccaagtcg aaaccgcacc
    27901 gcatcaccct gctggcgcca ccatcccgac attgtacgca acggttgatg cttgaatgag
    27961 cggaggtgtc caagccaggg cagtccaccg ggcacgtcag tcagcgtaaa ggaagggttt
    28021 gagtcgatat cctgcatgag tcaagaaaga gtcgattgtg gtatgcgagg aggggttatc
    28081 ccgcacctat gtgtccttct cgacggggag gggtcggttc gacaatgcac tctcctccca
    28141 tcaaagcttg gcatagtaca aacttcgtat ggagaatggt agggccgatg ggacctggac
    28201 gacggagccc cggtcttcgt tcaaggccgc ttcatgagct cgcatgtgac tgtgaatcgt
    28261 aactcaggaa tccctgattg atatcggaga tggtacgccc gcttcgacga aacaccccga
    28321 caccttcacg gtcgccagga agattcgtgt tcccttcgat ggtgaccagg cgatcatctc
    28381 gaaagtcttc cacaagaccc atatgtccat gcccaccgcc ggtgttgatc acaaagacca
    28441 gtcctggttt caacagggaa agatcgtcca ggatttgacc ggccaccaaa cgagtgacac
    28501 ctgcctgtcc ggctttcttc caatggtcca gcacgccggc tgtgcgaata gcgggattgg
    28561 tggttcccag ccgagttgcc gcctgcttga atgaccaata gacgaatgcg gcacaccagg
    28621 cgtaactccc cgtggaagga tcgattcccg ccgcctttag atactcatcc actttgggcc
    28681 cgcggttgga accggacgga tcctccatca cgccgacctg cgatgcggca atgctgagag
    28741 tggctttcat caacgcggag atcggcgtgg tggcgatcga aggcacccga tgtgcgccga
    28801 agagtgcagc ccaggtgagt ggcccgacga ctccatcgat cttgagcgat tgcccttgtc
    28861 catcgaccga gcgagcttgc aagagccgca cggcttcctg tgtcgctcga tcgaagacgc
    28921 cgctcacgga gattggcccg catccgacct ggttcaatcg ctgttggacg gcgcgaatcg
    28981 tcgagggcgc caggatacct gatttgaggg cgtatcctgg gtaatcgaga acggcgtctt
    29041 tggctcgggc tggtcgagta ggggaagcgg cacgggtgcg tcgcggcatg gtcagctcct
    29101 ggatgaaggt caaacgaagg gcttgattat tcccatcagt gaaagcatgg cgatcatttc
    29161 ttgagggcag gcattatacg gcagtcaccg caatttgttg cgagtttgat tggtccttgg
    29221 tcccacaatc gaatggcaac aggcggaacg ctgctaagga gtgtatcgtg actgaaaggc
    29281 gggcggaacc gggttctgcg ggtaggagat cggctgcctc ggaatgaggc gtgagcaacc
    29341 tggtttgtaa cgactctcag cggtcgcgca aatactgccg cactcgttcc acaatggcac
    29401 gatcgtggtc gatcattttc aagtcgtgcg tttcgtcggg gagaaaccag cccatggctt
    29461 gcccttctcc tagttcaagc ggttggccct ggtatcgttc aagaaacacg tatttcgtgt
    29521 tctcgtcttc gccttccctg aataattgtg tcatgagaaa gtgcgggtct tgtacgtgat
    29581 atgacaattc ctctatcacc tcgcgcttca gggcgtcgga tggactttct cctggctcga
    29641 tcccacctcc aaagaaggcc cagtaattgg gtaatcgata ggcgtcaggg gttcgatgct
    29701 ggaggaggag ttgtcccgag gacgtataga ggatgaagat cgcgacattt ctcacagcca
    29761 ttctgcacca tagcctatcc gcccaggcca ggtgaagaag atcttttgtt ctgaccgatc
    29821 atcggagtga gagcaagtct tgaccccttt ctaattgcgc ctccactagc cccatcactg
    29881 ctacaaaaat ctgatcagct cctgtgcatg tgaccctgat ctaggccatt tgctaggtct
    29941 tctcggggag gaatagcggt acaaggagcg gggacatcat catcctccgg gaggcaagct
    30001 catgagcacc accacggcag tcaaacaaaa aggacagacc tgcgcccatt atgggtgtgg
    30061 atgtcgggtg acagatgaag actattgcag ccctgaatgt gaatccgtcg ccagcggaca
    30121 gaccggttcc gccatctgcc cctgcagtca tcccaactgc aaaacccccc attaaggctt
    30181 gacgacgtct gggcgctgcg ggactcacgt tctgcacctg tatcgcgatg tctgtcgatc
    30241 attcgcggtc cgctgacttg gaagtgagaa gactgattcg tgttttcggc atcgagaagt
    30301 cgctcactgt ccgccaaatt agcgacattc ctagtgtgtt ctagcgaggg gcgtgggtac
    30361 tgtgtgcaca gcgtcaccgt ttacgagtgc tcacatggac ttcacatatg ctcacacctc
    30421 agcgtcctct caaactactg attatcgacg acagccccga ctttcagctg ctcatcaaaa
    30481 cctacctcga ggattttggc atcgtctgcc tcttcgcgac ggatgtcgtg caggcaacct
    30541 ccacagcggt gcgagaacag ccgaatctca tcctgctcga cattggcttg ccgggaggag
    30601 gaggcttgct cctcctcgag aggctcagag ccaaccttcg caccgcgaag gttcccgtga
    30661 ttgtgatcac cgcgcagacg accgatggat tggaatcaaa ggcgcgatcc ctaggcgctg
    30721 aagaatttct ccacaagcct gttgagaaag acgtattgat tgatacactc cggcgggtgc
    30781 tcagcaggcc acctacacag caggcatgag aggcgcccag ccatcggctg attcgttttc
    30841 aaagttccct tgttgggatc aatgttctct cttacgattc attcgatcga tatcgcatcg
    30901 tgcggtgcgt cacgctggaa cctcagacct gcgtccctaa tagtgtccct tgtcgtcacg
    30961 gcggtggatg atccagaaat ggctgaggac cagagacagg atggaaatga gacatcagtg
    31021 gtggtgtgag gagaaatctg caaagagtca caggagcgat tcatgattcg cctggcgagg
    31081 aagcccgacg gtatgttctc tgacctgtta gacagtgcga tcacacagat cgtcaatgac
    31141 tatctgacat cggcgaatgc acgggatcaa gaggattggc tctgcgcatc gatgcctcgc
    31201 gcggcccggt tttttactgc tccgcaagcg agagaacagt tgctcgccat gcagaagatt
    31261 taccatgatg aacgattgta tgaggtgacc aaatatcatt ggttactctt gtacgagtgt
    31321 ctggaaacct ttgcgcaggg attcaatgaa cagccggtgg gttggttagc tcaaaaatac
    31381 ggcattgaaa agatcgactt ccatcgcctg gttgacttgt ttttctggga cacctcgttt
    31441 ctgatggatc atctggccaa aatgagccgt caggaaacac ggacgatgtt gatctggccg
    31501 gaatcggtcg gacttgcggc cgggttcaag actcatccgg acgaactcgc gttcatcctc
    31561 tgcgacgaag gagttacaga agagttcgca gcccaaaaag acgttcctgc atggtctatc
    31621 gggtcaaagt cctacccctt gccgcacacc tagggaacgc gtattcatcc caggttctgt
    31681 atcccatcgt aaccaacgag gccaaatgtc ttggtattgg tcaatgggta tgtggaagga
    31741 gggaaagagg gaaggagatt gaggactcac agacactcac tacatcattt ttttgaatat
    31801 cgtcagaagc cgttggcagt aggtaggatc ggaggagaac tcggctccgg tgagcggcat
    31861 atttaagaac tcgttatatc ccggcagcga cctgacctcg ggcgaattca gatcgcgaaa
    31921 ccccatggac caatcaggaa attgtcgcgc gggtatggtt tgctgcaaga gtgtgagcac
    31981 tccgctatgt cgctcatcct gaaagatttt tgcatagagc ttcaacaccg cttcttcctc
    32041 tccctcaagg gcctgcataa agtttccatt cttgtagagc aagattccgg taatacctaa
    32101 tcgactgttg ttcgcatggc aagaggcgag caaatcagtg agctgtgatg gagagaatgg
    32161 tcttgtcgca gaactggcat acacaagaca aaacattatg gcctccctag gacggtcatc
    32221 tttgctatcg tccgtcgtaa aaaaactgcg ccatatgacg aataatgtgt gtgatatcag
    32281 gggtactctg ctgccgttgt tgcgctcgat caagtcaatt taactttcct tctctcacgt
    32341 tcaggcgtct ccgggctgac gagggtcccg acggaatagt catcttcaga tcaacccgcg
    32401 caaactagga acattgctag tccaactcac gtctctcgat ccttcataac ttttgtggaa
    32461 cgctctcagg gagagtcaca tgccaggtag tgcaggtgta aaatatgtcg cttgtgccca
    32521 ctggttaacc tttgtgaagt aagacacaag catgatgagg cctccacggc gccagtcaaa
    32581 agcggctctt gtcttcttgt tcgatgtaga cgagacgctg cttgatgatc aacgcgtgtt
    32641 gtccgatctt cggcgtcatt tacggaggga ggtcggacgt gaaggagatc gacgatattg
    32701 gaacatttta caacgcttgc accgagcatt gggctatgcg gactatctgg gtgccctgca
    32761 aaaatatcgt cagtcgtatc cccatgaccc tcatgtcctt accctatcct cctttctcat
    32821 aaactacccc tttgcgaaac gtgtttttcc gcaggccatg cgtgtcatta cacgctgccg
    32881 gcgtgtgggc tctgtggtca ttctttccga tggggatgtc gtgttccagc cgcataagat
    32941 tcaacaatcc ggtctcgccg cagctgcgga cgatcatgtc cttgtgtatg tgcacaagga
    33001 agagcaaatt cgcaacattg aggtgtgcta tccggcggat cactatgttg tcgtcgatga
    33061 taatgttcgt atcctcaacg tcatcaaaaa gatctggagg gaacgggtga ccaccgtgtg
    33121 ggtgaggcca agttcaagtg ggacaggtcg gaatggtatt cacgcgcatg cacaccccga
    33181 cctcaccatt cactctattg gagaactaaa caaagagcat ttgcgcgcga tcaagttgct
    33241 acagaaagag cagagtcact cttgaagagt cggcctgcac atctgagcac ctcgacacac
    33301 ttggtgcccc aaaatcgacg tattaattct tgccgttggc cttcccgctt tcgtccaggt
    33361 ttctcaaaac ctgcttgagc cgttgtttca ccagggtttc gagcagtgac ttcgcttcag
    33421 gatctgtttt aagtacctgt tccatcacca gttggcctgc cgtggctgct gaacgaagta
    33481 aaccctggtc gatggcattg acgatcatct catccatgga gcctggccta tgtttgtcgc
    33541 gccgtttatg tttactcatg acctctcctg cgtcgctcat gataaaggta tgcggttcat
    33601 gctaaaaaag tttccactca cacgtagaag gcgggcaacg tgccggttct ccttccagac
    33661 aaggcagcct tgtgactcct ttcccagctg agattctgga tgtcagagct tggcagcggc
    33721 acaatgtcgg tcgaagtgag tatcttgctg ctcgatggcg gaatgaatgc cggcttggac
    33781 cgtgacgaag ccaaatgata tctcagcgct tcttagctca tgaggcatcg aggtccatag
    33841 tcgcaaggta caaagacctg ggaatactgg gagcatccct ggttggcagt tgtcctgtga
    33901 gatccttggg ccggtgatca tgcatccaag ttgcagctcc ccagtgaagc ctgtgctgat
    33961 cttgccgtat cgttgaagaa tagagatgtg actccctcgc agtgcgagtg actatacgtc
    34021 acgatcttac ctcatcgctt atgattgggc tctcacctaa gtagtaggct catcattatt
    34081 ggctctggca tctgccattg attgcctgaa gtatgtcatg cagcagggat tcatgatata
    34141 agtgtttatg cgttgtcata atcggagtat agtactgcta ttgccgcggc gggattgctg
    34201 ttgtgcattc ttgacgtatc gttcaagacc atgaaaagga gcggtagaat gctgaattgt
    34261 tctgtgcatc agtgtcgtgt caggatcgta gctatacttg ccgcatgcct agggtggggt
    34321 ggactgatag gagtcagtta ctctgcttta atcacgtttc agtttgaagg aagtgtcatc
    34381 gctgtttccc cgtttttgag tccaccgttc agtacgtcca acacgttcca aggttcatat
    34441 acctttgatt cgctcactcc agacggaacc ccttcaacgc aagccggtaa ttatctgctt
    34501 acgaacgcct cggtaacctt gggaggcaat acgtattctg ccaatatggc gcccactgtc
    34561 actcctccgc tgatcacggt gattagcaac tcgtttggaa gacccactga ctttgcatta
    34621 tccgatgata gctatcgagt ctcatttcga ccatctggtc ctgcggtcaa tgaactgctc
    34681 ccgtcttcat tttctatgtc aatttctggc cttagacagt ttcccaatga ttccctcccg
    34741 ttaactccgc caagtctatc aggcagcgac acacggataa gcctgggctt tgttcagcca
    34801 ctaggtaaag ttggaggagt tacgggccaa ctcacctcgt tgacagttgc tccagtgccc
    34861 gtccccgccg ctgcgtggct gtttggaagt ggtttggctg cagtgaccgc gctggccagg
    34921 cgcaagcgaa aagcaaggct tgaatccacc ggctgttaat tcgtccgtta aaggagctgc
    34981 cccgggacat ggatttcatc tgcatgtccc gggctaccca ctccattaaa tcctttattc
    35041 tttgcctgcc actagtacaa ctcctgccat ccttgcttca aaaattcctc tgccgaatcc
    35101 ccacaatgaa cgggatcgtg tattgaccga tgaggagtga gggcgattgt atagtgaggc
    35161 caagacccgt ctgaggaggg ctctgctcac agcctatcaa tttggacaac gatttagcga
    35221 aattgtcgga ctcacttgag atcgtgtcga tgtgaagagg ggatttatca cccttcgttc
    35281 cctggacacc aagaccaaaa ctgctcgtca ggttcccatg acacccgatg tcaaaatgac
    35341 cttgcagcga cttgctcgga gacgcagtct tgccacgaga cacgtattac cgtataaagg
    35401 ccgacgcgat aaaacgcgtc agccgttctt tcaggaccgc cattgcgaag gctgatattg
    35461 ccgatttccg gtttcatgat ctgcgccact gcgcctccac taatctcaga agagcagggg
    35521 tggatacggc cactgccatg aaaattgtgg ccacaagtcc gacaagatgt ggaaacgata
    35581 caatacgatc gaggcgcgag acttgactcg ggctgcccaa aaggttcaca gatacctcca
    35641 agagaacacg ccgggaacac taggcgaaag ctctcttgga tactcatctc ataaatgatt
    35701 gaaaaataag tgtgcgcccg tagctcagtc ggatagagca tcagccttct aagctgaggg
    35761 tcgttggttc gattccaacc gggcgcacca taaaatcaac aggttgtgaa acagcccctc
    35821 caaggttact gagaccactc ccggttttac tcccggttcg cacttcggcc tgactttctt
    35881 gaggcgaagc aaacaagctg cccttggcca cggtctcctg catgaagccg ggcgtcagat
    35941 ggctgtacct cgccgtcgtt ttgatatcgc ggtgtcctaa aagctgctgc acgccgtaga
    36001 gactcgcgcc ggataatacc cgccgggtgg ctgccgtatg cctcagggaa tgccagcttg
    36061 cgccaacgat tccggctcgc cgtagcgctg ggtcaaagta ccatctgaca aatgagcggg
    36121 cgtccaaggg tttaagaggg tctttcccgg gaaatacgaa aggggacttc gtgaatgaat
    36181 cccacctgga gagtagccca cataccccgt cggtcaatgg cacataacgg cttttcgaac
    36241 ctttgggcat cggtaagctg aggactcgat tctccatgtc gatctgcgac catcggagat
    36301 taaactgttc cgatcgccgc aaccctgttt caatcgcgaa tgcgaccatt tcccacactc
    36361 gcggctccat caagtggcgg aggcgagcaa tttcgtcgtc tgtgagaaac cgggtcttga
    36421 tggcctctgg aaagaatttc acgccgctca tcgggttgcg gtcaaccatc agatggggag
    36481 caatccacgc tacctggtcc cgcctgatcg ctaaatagat cttggcatat tccagacaac
    36541 ggcgaatgta gtcctcggac tgggctaaga ggcggaacac agcggtattc aaactcctac
    36601 tctatcctga acatacgaag gaacggcttg tgtgagttct gccaattcga acgagcgtgg
    36661 gtacggatct gttttcgaga caacaacgtg acgtgggaga tctggttcag ataccaggcc
    36721 aatcgcccga catcagagag gtcctcaagc cctgctacga atcgagcatg ctctgaccga
    36781 ctttagcctt ccggttcgcg cgataggtaa gtcgaagggc ttcgttctta acagtactct
    36841 tcaacaaagc acaatgagac ttttttattg ctatagtttg gttttgagtt ggacctaaca
    36901 atgtgttcca gtattactcc tctcggattc cttgccccca ttaactagga cctctgacag
    36961 ccaagctagg atcgccctgg cagggacgcc caataggctt ctatagcaac acgaacatcc
    37021 agtttcgcat ttcgatgctt gcacaagtga agactaggga ttaacctagg gtgatccctc
    37081 gttgttcacc gtacgaacgc caacctatcc gcgtgtacgt tcccttcaga agaggagagt
    37141 aggatgcatc tcccgtgggg ctcccttcga cttcgcgccg ccaaggacta cggcctggcg
    37201 gttgtcatta cccttagtgc tctgctggcc cgctttcttc tcgagccgtt cattggcgac
    37261 cagcttcctt atgtcacgtt tttcgctggt gtggctctgt ccacattttg caccgatcta
    37321 gggccgtcgt tggcggctgt cattctcggt ggattagcgg cggattattt ttttctggag
    37381 cctcgctact cattcgtatt ggtcgggcaa gccaattacg tcggattgac aatgtacctt
    37441 ctaaccacgc tcgccattgt cggttttggg caaaccctgc gcgcattcag gcggcatgcg
    37501 gaacgcatca ccagtggatt gcagcaagaa atcgttgtgc gagaacgggt ggaactagcc
    37561 ttgcgcgaaa gcgaaacacg ttttcgatca gtcgctaata ctgccccagt catgatctgg
    37621 atgtccgaca tgaccaaacg ctgtatttgg ttcaataaag cgtggctcga gttcaccggt
    37681 cgtacgatgg acgatgagct tggcgaggga tggatggagc tcgtccatgc tgaggatctc
    37741 gcccaatgtc tccaggtgta tgaaagcgca ttcgatgctc gcgagacgtt cactatggaa
    37801 tatcgcctcc gtcgccgtga ccaagaatac cgatggctgt tggaccaagg atgtccacgc
    37861 ctggatccag acggtacgtt tcttggttat atcggatcgt gtgtcgatat taccgagcgc
    37921 aggcgtgccg aagcgcagct ggtcgagagt gaggaacggt ttcgtacatt ggccgacaat
    37981 atgtcgcaat tcgcctggat ggctgatgcc agcggttggc tcttttggta caaccgccgc
    38041 tggtacgagt atacgggcac gacttacgag gagatgaagg gctggggttg gcagaaggtg
    38101 catcatccag actatctcga ccacgtgatg gagacatggc gaaaggctca tgccacaggc
    38161 gagccgtggg aagatacgtt tccattgcgc ggtaaagatg gcatgtatcg atggtttctc
    38221 tctcgagcag tacccattcg tgacaatagt ggctctatca ctcgttggtt tggaacaaac
    38281 acagatatta ctgagctgcg gaatgcccat gctgcgttga agcaatccga agcacgatat
    38341 cgcgctttgt atgactcgat tgatgaggga ttctgtatca ttgaggtgat gtttgatgaa
    38401 catatggggg cggtggattt tcgcttcttg gagacgaatc cggcgtttga acgacagacc
    38461 gggattcaga atgcacgggg tcaacgcatg cgggagattg ccccgcagca tgagatgcat
    38521 tggttcgact tgttcggcac cattgcgttg agcggagaac cgaagcggtt tgaatatcca
    38581 gctgtcgaat tacaccggtg gtatgaaggg tatgcctatc gggtgggcga agaatatgaa
    38641 cgaaaagtcg gcatcatctt caatgacata actgtacgga aacaggccga agaaaaactg
    38701 cgtcagagtg aacaacggct acgtctagcc ttatcggcag gccagatggg cgcctgggac
    38761 atcgaccttt tgacaggaag tgtcacctgg gacgcaagac agtgtgattt gttcggtatg
    38821 tccaaaaccg acgaaccggt ggatatgaac aagtggtacg agctcgtgca tccagacgac
    38881 gtaggaccgc ttagggccgc gactgcacgc gccgaaacca ctggttcgtt tgcatgcgaa
    38941 ttcaggatcg tgcagccaca tagcgcggag cgttggattg ccggcgtcgg tgcgacactc
    39001 aagaacgaga caggacaggc agtgcgtatg gtgggcttaa attacgatat cactgagcgt
    39061 cggaagaccg aagctgactt gcggacgtct gcggaagaat tggagcatgt agtccaggcg
    39121 cgcacgcagg aactcgtgct gtcacaagaa cgactacggt cactggcggc tcagctcaat
    39181 ttggccgagc aacgagaacg gaagcgtctc gccggcgagc tgcatgattc tctggcgcaa
    39241 tggctcgtgc tctgtcaact ctacctcggg caagtgagtc gtggatcttt gacgtctgat
    39301 gttggcgaaa aaatccggca gactgaggat ttgctgaacc aagcgctaaa ttacagccgg
    39361 acgttgatcg ctgaattgag tccacgagtt ttacaggatc atggcctcgt tgctggcctg
    39421 acctggctcg gcgaggaaat gcaacggcat ggtctagcgg tccgagtagt tgcgaccaaa
    39481 ggcgttgaga cgacactatc agaagattca agcgtgctac ttttccaatc tgtccgagag
    39541 ctactcatga atgcgctgaa gcatgcgagg tgcaatgaag tggtggttcg tctccatcga
    39601 gatgagggca atttatgcat cgaggtgcag gatgatggtg tcgggttcat cgccacttct
    39661 aatgcggacg aaactctttc gtcaaaattc ggacttttta gtatccaaga acgcatgacg
    39721 gcgcttggtg gaatgctcaa tgtccaatcc cggcctggtt acggtaccat agccaggctg
    39781 ttgctgccat tgaccagcag tgcgaccggc ctacagcccg tgaagggagg ggcgacggtt
    39841 attcaggctc attccgattc tgctcctcct tctctaaatg tcaccgcacc gattcgcacg
    39901 ttattggtcg atgaccatcc catgatgcgt caaggtttgc ggagtctcct aaccactttt
    39961 gcaaacattg atgtcgtagg cgaagcgtcc gatggccagg aagctgttga gttagcggaa
    40021 cgacttcggc cgcgcctcat catcatggat gtgaatatgc ctagaatgaa cggcattgaa
    40081 gcgacggcgg tgatcaagtc tcgtttgaca gacacgagcg tgattgggtt gtcggtgcat
    40141 gcgagtccag agactgatcg cgcaatgagg gccgccggtg cggctatctt attacccaag
    40201 gagacggcgg caaaagcgct atatcaagcc atccaaaagg ttttgacgga tggactcaac
    40261 attccggatg gtgcgatggg agattgcata aaagcctaat ccctgcctta agcgacgata
    40321 atgtggctga ttggcattgt gcaggatcat agaggccgac ccggggtcgc tgtgttacag
    40381 cgcttcttgc gcggccgact gtcctctctt ctcatggata tcaggaaatc caaaaaattg
    40441 attgaaggag tggattaggc cttacgtacc ttgaaacttg cgtcaagacc cttcagtttt
    40501 tccataatac cggtatattc caattctgcc ataggtagca tggccgcacc gaagaacccc
    40561 tggcgtcgca tgctgatggc tttctctgag acgttgtcgc ggacccggtc ccaaaatggg
    40621 tggacaaacg tatccacagg ctcggtaagc cgtcccgcat gcatcgcaaa ggccgcacag
    40681 gtaatcactg gcggaccgtc gaaatagctg atgccagact gaagaggtac tggcatcaaa
    40741 ggcatttggt gagaaccccg catcccccct gctacatacg ggccaatggc atatggtgcc
    40801 aacacctcac cggtggcagg gaaatccttt tgcacccgga cgagcatcac cggatcgtcc
    40861 ttgccggtat acttgccagc gatgttatgc agccgagaag tggaagccgc cacggcttgt
    40921 tcgccgctgg agagggacca tattgactcc acgacgtagc gctcggtgtc ccgcagcaat
    40981 gctgcaatct cgtacaaatc tttcggggca gaaagctcga tgattcggtc gccatccgtg
    41041 tgattaacgt ccattatgac gaaacgaaag cctcgcgcca tgttgggtgc cagcatcaaa
    41101 cccggagtgt tcatagggtc ggcgaaggca agatatagcg ggagattaaa ggccccgggg
    41161 tctgtcttgt cggccgcgaa aaacaaaaag ggttcgtttg gccgttcgtc gaattccatc
    41221 tccgccaccg ctggccccat gccgtgcaca ttgcctgaaa aggcgtcctt caacaaatcc
    41281 tgtccggcac cgtatagacc ctgcttttta gccacctccg tcccagcgac aaaggcgtcc
    41341 caggctagtt tgtgaatcgc ttcatgcccg gggccgtgcg tgtggctcat gagaatggcg
    41401 atatcatcac ccgtactact aagataatgg tcgatgagca acggtgaacc gtgttgggca
    41461 atatagctcc tcacggtttg tagcaattgc tgcgagggac aaatgtggcc accgatggac
    41521 ccgatgtccg ccttgatgat gctgagggtg attttcatgg ccgcagctcc cttaggatgt
    41581 ggagggcatc cttcgtggat aaccgagagg gatgagcgga tgcctcgaaa tttcatccct
    41641 gagcatatgc aatccgtgca cggaaataac cacccactgc tgaagaagtg ccaactctat
    41701 ggcgtgcatc atctgtgccg tttgggaaac ggagtacttc tatcgccgtc aagcctcgga
    41761 taggtgcacg tacaatgtct tttttttcaa aacgaagtgg cctccgaatg cgatgggacg
    41821 cattggagag tgctgtctgt ttcgctatgg ggcggcgagc gaagttgaag gttgtacatg
    41881 gctcgatctc ccccgggctg atgtgacgcc gatctcacgc agaatgatcc gcgtcttcgt
    41941 acctcttagg atgcagggtt gaaagggagt agagcaggcc tgatgttaaa actgttcgga
    42001 agtaagccct ccgcctaagt cgcagccaca ccgacgatgg ttgatgcgtt tcgggccatt
    42061 ttgaattcag cgggttgtag ctaaatacct cagcgacagg gtgccgcttt actcccggtt
    42121 gactcctaat tttgctccca gttgccagtc ttacataatc ccactccgta acactgcaac
    42181 aaccattatc cccttatcca tgtggcatta gggctatcct tactactccg tctttcttaa
    42241 tccccgtgct gtccggtgga tcttctaagc tgagggtcgc tggttcgatt ccagccgggc
    42301 gcaccacaag agactctacc ctaggttttg gaacctcgaa cagctgcctc gattgtcgcg
    42361 acgtcgatct ttcccatttg catcatggca tcgaatg
//
LOCUS       DAOQDS010000037        41432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_304_length_41432_cov_22.185296, whole genome shotgun sequence.
ACCESSION   DAOQDS010000037 DAOQDS010000000
VERSION     DAOQDS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 41432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41432
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_304_length_41432_cov_22.185296"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PK589_10745"
     CDS             complement(<1..931)
                     /locus_tag="PK589_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ20122.1"
                     /translation="MEPILPLRNDIGLAVRPIFSAGMSRLGWVLFFWLLIAPAYAAEP
                     SSTPGDSPAVSADSGLWHYGAYLDVAYILNFNFPENHLWRNRATAARHNEFAPNMALA
                     YVRKDVNESSRWGMELGVQGGYDSERFAFLQGEREVSGADTLRHIHRANVSYLAPIGK
                     GLTITAGLFNSLIGYESLYAKDNANYTRSWIADNTPYMMFGVNAKYPVSDELTITAFV
                     INGYYHLSHPNDQPSYGGQWAYKATPRLTLTQTLYGGPDQSNTALQFWRFYANHIVEW
                     KGDDLTLALSYDIGTENIADRPGHPRAFVTGGNM"
     gene            complement(1054..1761)
                     /locus_tag="PK589_10750"
     CDS             complement(1054..1761)
                     /locus_tag="PK589_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ20123.1"
                     /translation="MPSTTTHEVTVLLIEDEAEIRRFLRTTLPPYGYRLYEATTGADG
                     LAQISARNPDIILLDLGLPDMNGTAVIEKIRAWSNMPIIVLSARDQEQVKVQAFDLGA
                     DDYVTKPFGVDELLARMRAALRHAHRPIGDAVEPVCTFGEVTVDLGRRQVFVSGTEVH
                     LTPIEYKLLTTLVRYAGKVVTHRQLLKEVWGPLHVDEGHYVRVYMRQLRNKIEADPAQ
                     PRHLVTELGVGYRLRAE"
     gene            complement(1774..4482)
                     /locus_tag="PK589_10755"
     CDS             complement(1774..4482)
                     /locus_tag="PK589_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HNJ20124.1"
                     /translation="MENQRPDPDLLLRRVQAEEADQTRGRLKVFFGATAGVGKTYAML
                     QAAHEQQRDGIDVVIGWVETHGRAETEALVHGMPTLPPREVVYGGSRLRDFDLDAALA
                     RRPQLLLMDELAHTNAPGSRHPKRWQDVRELLQAGVHVYTTVNVQHIESLNDAVAQIT
                     GIRVSETVPDAVLEQADDVELVDLPPDDLLQRLKDGKVYLPDQAQHAIKHFFRKGNLI
                     ALREMALRRTAERVDQQMEVYRRDHAVVGTWPAAETILVCVNLKPRGPMLVRAARKMA
                     TGLHARWIAVYVQTSKHLRLSDQERNQGVDTLRLAERMGAETVTLTGEDVSAQLLAYA
                     RSRNATKIIVGKPLRSRWKEWLFGSVVDDLVRGSGDIDIYVMTGEGGTGKPLVKGLLQ
                     RTSERRNYGYASAVVGACTALSLVMSPYFGQANLIMVYLFGVVVVATRWGRGPSALAS
                     LMSVAAFDFFFIPPYYSFAVSDAQYLLTFAVMLLVALLISRLAAHKSQQAEAALIREK
                     RTAALYAMSRELITQRGVDKLASVASRHIHDVFHCQVAVFLPDREGRVHLHRGEALHF
                     ELDPKEAGISQWVFDHKESAGHGTNTLSGSDSLYLPLLGSHGAVGVLAVRSSRPAELL
                     APEQLHLLETFANQMALALERARLAEEAQEAHVQTETERMRNAVLSSVSHDLRTPLAT
                     IVGASSALLSTTRPLSTESQQDLVRSISDEAGRLDRLLKNLLDMTRLEAGAMHLRKEW
                     HALEELVGTALARVKSRIATHTVTTAFPVNLPLVLVDGVLIEQVLINLLENAAKYSPT
                     ASPIEVTASVKDGVILIEIADRGPGIPQGEERRIFEKFYQLHPEREGGVGLGLTICRG
                     IIESHGGRIWVDSRSGGGSSFRFTLPAEKSQPTVEPEANQHDSAAI"
     gene            complement(4568..5149)
                     /gene="kdpC"
                     /locus_tag="PK589_10760"
     CDS             complement(4568..5149)
                     /gene="kdpC"
                     /locus_tag="PK589_10760"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HNJ20125.1"
                     /translation="MAAQLRSALMVFFILTILTGVAYPLAVTGIAQLLFPHQANGSLI
                     YKDGKPIGSTLIGQPFDDPKYFWGRPSATAPFPYNASASSGSNLGPTNPALTEAVKTR
                     VAALKAADPRNDAPVPVDLVTASGSGLDPHISPAAAEYQMRRVARARGKDEAVVRTLV
                     IQHTEGRQLGLLGERRVNVLALNLALDASGQAR"
     gene            complement(5160..7271)
                     /gene="kdpB"
                     /locus_tag="PK589_10765"
     CDS             complement(5160..7271)
                     /gene="kdpB"
                     /locus_tag="PK589_10765"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HNJ20126.1"
                     /translation="MTTSTKARPLFDPPLVRQALLDALRKLHPRIQVRNPVMFVVFVG
                     SLLTSLLFFQALFGAGEAPTWFILAISLWLWFTVLFANFSEAIAEGRGKAQADSLRKS
                     RTELTAKRFGTPASGGDYLAAPAAIGKPGEVYSLVPASLLKKGDIVLVEAGDYIPSDG
                     DVIEGIASVNESAITGESAPVIRESGDRSAVTGGTRVLSDWLVVRITTSAGETFLDRM
                     IAMVEGARRQKTPNEIALNILLAALTVVFLLATVTLLPFSLYSVHATGQGAPITVTVL
                     VALLVCLIPTTIGALLSAIGIAGMDRMVQANVIAMSGKAVEAAGDVDVLLLDKTGTIT
                     LGNRQATAFIPAEGVPETSVADAAQLASLADETPEGRSIVILAKERYGIRGRDIHAMG
                     ATFLPFTAQTKMSGVDLDDRQIRKGAAEAIEDYVTQQGGSFPPAVRDAVETIAMQGGT
                     PLVVAEGKTVLGVIHLKDVVKGGIKERFAELRRMGIKTMMITGDNQKTAAAIAAEAGV
                     DDFLAQATPEAKLKLIRDLQAGGRLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEA
                     GNLVDLDSNPTKLIEIVEIGKQLLMTRGALTTFSIANDVAKYFAIIPAAFATTYPALG
                     ALNVMGLATPESAVLSAVIFNALIIIALIPLALRGVKFRPIEASLLLRHNVLVYGLGG
                     IIVPFIGIKLIDLLLVALGLV"
     gene            complement(7295..9100)
                     /gene="kdpA"
                     /locus_tag="PK589_10770"
     CDS             complement(7295..9100)
                     /gene="kdpA"
                     /locus_tag="PK589_10770"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNJ20127.1"
                     /translation="MTINGLAQIGLFFLVLAALVKPLGWYMARVYTGQACGMDRVMGP
                     FERLIYRVCGVREAEAMNWTSYAVAMLLFNVAGLVALYALQRLQGLLPLNPSAFGAVA
                     PDLAFNTAVSFITNTNWQAYAGESTLSYLTQMLGLTVQNFVSAGTGMAVLVALIRGLT
                     ARTASTIGNFWVDLTRSTLYILLPLSAVLALVLVSQGTVQTFGLYHQTALLQPVTYDK
                     PVVDAAGQPVVDEKGVAKMESTTGIEQVLAVGPAASQVAIKHLGTNGGGFFNANAAHP
                     YESPTPLTDFILILAETLIAASLTYTFGMMVGDTRQGWTILAAMLSVLAFFVLGAYWA
                     ESAGNPRLTALGVEQNVQIAQSGGNMEGKETRFGVARSALFATVTTATSTGAVNSMHD
                     SFTPLGGLIPLLMMQFGEVILGGVGSGLYGMLVFAIIAVFIAGLMVGRTPEYLGKKIE
                     AYEMKMAALLILIMPIVVLGFTAVAVVTESGTLSILNAGAHGFSEILYAYTSQGNNNG
                     SAFGGLNANTPFYNVTGALAMLVSRFWLAIPTLALAGSLARKKMVPLGLGTLPTHTPL
                     FVVLLIGVVIMVGALTFVPALALGPVVEHLLMVAR"
     gene            complement(9100..9189)
                     /gene="kdpF"
                     /locus_tag="PK589_10775"
     CDS             complement(9100..9189)
                     /gene="kdpF"
                     /locus_tag="PK589_10775"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HNJ20128.1"
                     /translation="MNAMYVLGAVLSLGLLVYLMVALLKAEWF"
     gene            complement(9203..9472)
                     /locus_tag="PK589_10780"
     CDS             complement(9203..9472)
                     /locus_tag="PK589_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20129.1"
                     /translation="MGKIFSEIHFLRFFTPFLLSARYLEHVVEEKGRAAMWLVGDGLE
                     RDSGEARLNTCAVSLRNIDMDLIYLAIAAVFFLLSGWLISALDRL"
     gene            complement(9642..10163)
                     /gene="nuoB"
                     /locus_tag="PK589_10785"
     CDS             complement(9642..10163)
                     /gene="nuoB"
                     /locus_tag="PK589_10785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNJ20130.1"
                     /translation="MFRIIKKSLTTGVVTGQYPMAKASQEPVSREAIEKVKPFRRSLT
                     IREVDTGSCNACEMEMNALANPVYDVERFGVEIAASPRHADALVVTGPVTVNMERALK
                     DVYKQTPDPKLVIALGDCAITCGMFKGSYAVTGPVDRHIPVDVRIAGCPPRPVEILAV
                     LAELRKDCPDSDQ"
     gene            complement(10220..11812)
                     /locus_tag="PK589_10790"
     CDS             complement(10220..11812)
                     /locus_tag="PK589_10790"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNJ20131.1"
                     /translation="MPDGLTAAARVQEAFPQGIIQVGSMQGVPMLRLRKPDVPAVARY
                     IHTDPELRGSLSLLWAIDHRPRDARDELWYLFTLAARKDWLLLTVDLAEEEREFASIT
                     PMVHAAKWYEREIRDLFGLIPVGHPDMRRLVRHEHWPKGMHPLKKEFAWDQVLERAQG
                     EYAFRKIHGDGVFEIPVGPIHAGIIEPGHFRFSVAGEPILQLEVRHFWKHRGVEKLFE
                     QQRLTEAVPLAERVSGDTSVGHSLAYCQAVETLLGMTVPPRARYLRSLFLELERLHNH
                     IGDVGAICNDTAYALAHAHCGRLKEQLMQLNDGLTGSRFLRGVNRVGGVSIDLSGVQL
                     SQVDAELQAIEQEFSGIEKILLANASLTERLENTGVLSESIAWDHAVMGVVGRASGID
                     RDLRRDRPFAAYPECQPKVAVYRYGDVRARMRVRLDEVHDSMRLIHTLRRQLPMGPLA
                     TQAVEPVTQGQWAISAVEGWRGEILYVVMAGEEGRIHRCKVRDPSFVHWPAIQWAALG
                     NIVPDFPLINKSFNLSYAGNDL"
     gene            complement(11818..13350)
                     /locus_tag="PK589_10795"
     CDS             complement(11818..13350)
                     /locus_tag="PK589_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNJ20132.1"
                     /translation="MWPVIVLLAGPVLAGLLSLVIQRARLLHAVNFTTMLGLAVAETF
                     LTKRVLAEGSVTTLGSLVYIDALSDFILIIITAIGLSCSLYMWSYMDEQVARAVIAPK
                     HLGVFFFLFHMFLFAMVAATMANSLGVQWVAVEGTTLATTFLIAFFRRRESLEAGWKY
                     LILCSVGIALALFGVVLTYYSSVRVLGDASSALNVTALIGVADRLDPNVLKLAFLFIL
                     VGYGTKVGLVPMHTWLPDAYTEAPAPVAAMLAGVLETVAVYTVLRSKALVDQALPPQF
                     TGNLLLTFGFVSFVVASLFILLQHNYKRLFAYSSIEHMGLAMLGFGVGGLVGTFGGLF
                     HLLNHALAKALAFFVAGNIHRRYDSLEIDHVRGLGRSQPITALATLVAGFALVGLPPF
                     SPFVSELLVVSALSAQNFASDTMHVGRFVAVTVSDEMRSLGLVGVFLFFGVVLFGGMM
                     ARIGAMVWGTPQDEIRRGEPWTVGHVPMLAMIVALLGLSVMLPESLRTLVTRAVHVIV
                     VR"
     gene            complement(13387..14052)
                     /locus_tag="PK589_10800"
     CDS             complement(13387..14052)
                     /locus_tag="PK589_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNJ20133.1"
                     /translation="MSESTQLGSQLVDLCSVLLLLSCFAIVAQRRLSACVDLFALQSF
                     FLATTAALVAFLTGHRHIYLAAALTVVIKVIVLPRILRKVIERLNVSRELVMNINIPA
                     GLLICGGLVVVAFFITQPIIPLGYLLTRDSLALALAIILIGLFTMIARKKAVTQMVGF
                     LVMENGVFLGTTAAAYGMPLIVELGVFFDVLVAGLIAGIYTHRLQDAFDSVDTSELTV
                     LKE"
     gene            complement(14057..15013)
                     /locus_tag="PK589_10805"
     CDS             complement(14057..15013)
                     /locus_tag="PK589_10805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNJ20134.1"
                     /translation="MQVISQIGFVFIQLIILLAFSPFIVGVIRKVNARLQCRRGASLL
                     QPYADLAKLFGKQPVISSTTSWIFTAAPYIVFASTVAAGLLMPVFISHTPLNFAGNII
                     ALVYLLALGTFFLILAGLDAGSAFGGMGSSREAIVVTLAEPAMILSILAIALTAGSTN
                     LSTIVHQSALLEGMVLAPPAHLMALAAMFIVTLAETGRVPVDNPATHLELTMIHEAML
                     LEYSGRYLALMEWAAGIKLLVFLTLLVNVFAPWGIATEATLSALATGVAVYVAKVTGL
                     AVIIGVIESMFAKLRLFRVPELLGSAFILALLALVFFYTLRG"
     gene            complement(15076..17100)
                     /gene="hyfB"
                     /locus_tag="PK589_10810"
     CDS             complement(15076..17100)
                     /gene="hyfB"
                     /locus_tag="PK589_10810"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HNJ20135.1"
                     /translation="MTTLLTVLLGCYLLGGLLPLCTSRPHLQNILAHSAAALAGGAGI
                     ALGLLGLVASQPLTISVPSNIPYLTFALRLDPLAAFFVLTISLIGMAASIYAYGYVRE
                     FTGRVSIGLLGSLLNGFLLSMTLVVLADNGFFFLIVWEVMSLLSYVLVVTEHERPGVR
                     EAGLFYLIMTHAGTAFIILTFLLFSQETGSFSFEAFRHPDQRLPDGLRSIAFFTALIG
                     FGAKAGIVPLHVWLPYAHPAAPSHISALMSGVMIKTAIYGLIRVYFDFLEGQFPWWWG
                     FTVLSIGAVSALLGVMYALMEHDLKSLLAFSSVENVGIILLGIGAGMIFHTYGLHELA
                     ALGLLAGLYHTINHAAFKGLLFLGAGSLLFVTHTRNIEEYGGLLRRMPWTGLCFLIGA
                     VSIVALPPANGFVSEWLVFQSLFLSVQLPTLFLKLMLPIAAAILALTGALALACFAKA
                     FGLSFLAQPRSAHARHAVEVPVSMRIGMGLLASSCALFGLAPMLVVPLLDRVTTPLAG
                     VAISEKVLALDGWVVAPVTVEFSSISMPVLAALLVGAAGLGLAIARLLRKGVHARYSR
                     TWGCGLPLTPRMEYSATGFVQPIKQVFETIYQPTVKLEREFLEQSKYFIRHQRFHSSI
                     EPLFEKYAYRPIVNGLLAAAERLRVIQAGSLHLYLAYIFVTLVLLLLWAG"
     gene            complement(17352..17807)
                     /locus_tag="PK589_10815"
     CDS             complement(17352..17807)
                     /locus_tag="PK589_10815"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNJ20136.1"
                     /translation="MFASLIFLWAGVSLSAVHAEEAASRFTLVLNGEAVKDSASGLVW
                     EREPDYIFDVWDRSVARCATKTVGGTQGWRAPSIDEIKTLVDLDQQDPSLPAGHPFRN
                     IKSGIYWTATPHPKDEIVAWQQSFFSGQSVTDQKSGTRRLWCVLGDAGK"
     gene            complement(17898..18620)
                     /locus_tag="PK589_10820"
     CDS             complement(17898..18620)
                     /locus_tag="PK589_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20137.1"
                     /translation="MHCPKCGWDQDEGTADCARCGIIFAKMHRSPAPPVMPRSPGQDS
                     TRPRVVEEWLLITEDSVNPFYFAGRVLVFLLLVSWGWRFITTPLETNYTGESFLHLIN
                     LPFHEAGHLIFMPFGRFMMILGGSLGQILMPLICLGTFLLKTRDPFGGSVALWWTAQN
                     FMDVAPYINDARAMDLILLGGFTGKEVDAHDWNNLLTMLGWLQYDHGLAKLAYGVGTV
                     LMLVSLLWGASLLRRQYRRLDW"
     gene            complement(18624..19583)
                     /locus_tag="PK589_10825"
     CDS             complement(18624..19583)
                     /locus_tag="PK589_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNJ20138.1"
                     /translation="MNRDESDQNRSSGELISPSFENAEEDEWVEEERPAFSAFVLPIM
                     LFALTLFTVLWAGAYQTNTNPLVGPWDFLVDDPGSLWRGIPFAATLLGILVTHELGHY
                     VLSRLHGVPTSLPLFVPGLPHFVGTFGAIIRMRAPLTDRRALFDIGVAGPIAGFVVAV
                     IALVIGLRLSTVVPIQGNYGMHLGEPLLLQFASWVVIGPLAPTADVILHPVGFAAWFG
                     LFITSLNLLPIGQLDGGHVAYALLGERQRSVAVALIPILMVFGWLGWKGWFLWVGLAG
                     MMGLAHPPVRNPGRDLGTARVLIGWLALFIFAVTFSWEPFILR"
     gene            complement(19599..20435)
                     /locus_tag="PK589_10830"
     CDS             complement(19599..20435)
                     /locus_tag="PK589_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ20139.1"
                     /translation="MPLEDELSDIIKKARTGQQRSVADVARAAGFPEADLAGLERGQP
                     ARNREQVQAVGRALGLRADQLAQVAEGWTPEALPASTPHVDTILGSIGGYEVKGYIVH
                     DRGETVIVDTAYNADAMLETLAKRKLRLRAICLTHGHSDHADGIDAILKAHPVPVYLG
                     PDDLDLLSWRPSQDVLHVPKEDGTLQVGGLRLRFMPTPGHTPGGVCYRLEQAGEPMCF
                     VGDTLFAGSIGRSNPAGLYQTHLASVRQRVLALPPDTRLFPGHGPATTVHEERLHNPF
                     AT"
     gene            complement(20641..22068)
                     /locus_tag="PK589_10835"
     CDS             complement(20641..22068)
                     /locus_tag="PK589_10835"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ20140.1"
                     /translation="MSCPVFVLLFLVQASLVMAGDLVLDNPRSMRFPTVEFNPPDAER
                     LVLDNGMVVYLLEDHELPLVTINATLKTGSWLDPADKVGLAGMTGAAMRTGGSASMSP
                     DEVDEELEQLAATISIGFSKESGSASLDVLKKDLKRGVQIFSDLLRRPAFEQSRVELA
                     KLQALEGIRRRQDSPGSIVGREFAKLLYGPTHPSARETSVRSIESITREDLVAFHQQT
                     VHPNGIILGVTGDFDKAGMLALLREAFGDWAKGTVPDVTIPAVAESEFKTGVVRFVNK
                     DTSQTHLRVGHLTVKETHPDYVAVAIANDILGGSSFRSRLFNDVRTKRGLAYSVGSGL
                     RASVYDEGVWLMRAETKLSSTQEVVNRFVANMERMRNEPVTDAELEEAKEAYVNSFVF
                     SFTSASSIVARLMDLEYDGLPKDWLQQIRDKVVKLTKEDIQRAAKMHFNPDRLRILAV
                     GSGDALSKVLASFGEVKEITLAPES"
     gene            complement(22125..23696)
                     /locus_tag="PK589_10840"
     CDS             complement(22125..23696)
                     /locus_tag="PK589_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ20141.1"
                     /translation="MISITRARTIGVVILSGILAAAAPGYAESPSLADRVIEHKLANG
                     MTVLMVERHQAPIVSINMTFGVGGVNEQVGLTGLAHLYEHMAFKGTRTVGTRDYEREQ
                     AVLDELTVVGNELDHRERDEAARAQMEGKPHVASPEVQQLQRRFKELQEKAGEFVVGN
                     EMALLYQRHGGVGLNASTGKDITRYVISLPANRLPLWASLESDRMAHPVLREFYKERG
                     VVMEERRLRTDDSPNGLLYETFTSTAFQAHQYGVPTIGWGSDIMALTPAATEAFFKTY
                     YGPNNATVAIVGDIQPKEVIALIEQTFGKIPAAPPIPALVTEEPPQRGERRVEIEFDA
                     EPALAIGYHKPTIGHPDDFVFDVIDEVLTEGVTSRLYSTLVRDKRLAASVLSDTNYPG
                     VRAPNLFVIAATPLAPHTTAEVESAIYEELERLKTEPISAKEFERVLNGLDADLVRSL
                     RSNSGLASQLAFYQTVAGSWRYVLNARARIAAVTPADVQRVAAQYLTKPNRTVGVLVK
                     KAPDKKMAAVGEVAR"
     gene            complement(23693..24343)
                     /locus_tag="PK589_10845"
     CDS             complement(23693..24343)
                     /locus_tag="PK589_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNJ20142.1"
                     /translation="MTKKSEIDLLIFDLDGTLIESKWDIADSVNLTLRDLDLPERPQE
                     EIFGFVGDGVKKLLRLAVGEGNQDRFDQALRVFRGHYLEHCLDRTVFYPGIDRVLAHY
                     AAKSKAVATNKAIEYTNVILKGLGHHHFAYVVGGDNGFGLKPEPGMLVHVMEQLGVKK
                     DRTVLIGDSTNDINGGHNAGIRVCAVGYGMGNRQKMAACQPDWFIERPEELMELFI"
     gene            complement(24340..24900)
                     /locus_tag="PK589_10850"
     CDS             complement(24340..24900)
                     /locus_tag="PK589_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20143.1"
                     /translation="MISLRGAIIGIMAGIVGLTAWASPGHAQSNAVSHSPAKVVEKYF
                     ALDNKGVRLDASSFESVAGYVDWKEEPAWGKVVVINGFTVPDDFRQWEIVNRLEVVVP
                     VEFRVLGIMYLDTAGFVPEPGTEQARVRLKVMNGRWKIMEPILPPHVGQKRMLNVVRQ
                     AMLEEKDGTRQASLAALQAELRKAKE"
     gene            complement(24905..26356)
                     /gene="pyk"
                     /locus_tag="PK589_10855"
     CDS             complement(24905..26356)
                     /gene="pyk"
                     /locus_tag="PK589_10855"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNJ20144.1"
                     /translation="MRKAKIVCTIGPASDGVGVLEQLIQSGMNAARLNFSHGTHASHG
                     QAIKVIREAADRQGVAVAIIQDLQGPRIRVGEIDGTLELRAGQRVRLRTMTLRSGGQI
                     GARNVLPADSAQDIPVTYQALTRDIRPGAKILIDDGLVELTADRIIDGAVECTVVAGG
                     RVTSHKGMNLPGTIVSAPTLTDKDREDLRFGVAQGVDYIALSFVRGADDIRAAKRLIA
                     ECGGDVPVIAKIERHEAVTDLDAILQQADGVMVARGDLGVELGPEAVPVLQKRIIATA
                     NRHRRLVITATQMLESMTQHTRPTRAEASDVANAVFDGTDAVMLSAETAVGHYPVEVV
                     QVMDRIVRAAEVETGPAFVRRASPDNRHLTFEEAICLSASSAAAATGATAIVAFSERG
                     TTARLVSKQRPAAPIIGFTPFEPVRRQMALYWGVMPHTMPQIPTTDERVNEAERRLKA
                     DGLATAGQRIVILSGTRIGQPGGTNLMKLHEVG"
     gene            26564..28903
                     /locus_tag="PK589_10860"
     CDS             26564..28903
                     /locus_tag="PK589_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNJ20145.1"
                     /translation="MSASDKQATEQLDFVTLEGLLTYGTALAQRSSSGRYALPPPPPP
                     SADRLQPAQAAVEGMRAFIQHVQKGLPSAEAYRAARQTLLAKTCGNDPVVFFAAWNLL
                     LARGELAPLYRAPIGATQKPAHRRPVAIVPRTQLTPQLAEGRIVLDLGDDRFWLLPRD
                     LGDRTLFLTMRHGVSEVESKTHRVGRRLANVLDTERGVPRADAVGAALARMVGVVGQQ
                     LGYLHLRNYLDPRTFLHLISHSPNTEQLCRRITKALLPDGADAVHPHIEPTLESQDFG
                     WVTGMEKQAELDAAAQAFGVDAKTAKRLIKHPFYSYPGGHSFFDLYVDVIDGLHRLGH
                     AHPGEVLCLYTHSSTLRALRIYLDPRPFREAFSEFGEYKEGQDNVVLLTLENGKLSGY
                     STAVGLIESDRVAREAWVTVERERTQRVTLKPGRIKRLIALVSGGDFGGGGAALKELR
                     VTGNRFGLDVFFVRHGFLGLANNWIDAVTEEDTRGMSSHASSPIGSSRFEDFKDEQVQ
                     RAAMRSLAPYLEDSALVVLGGDGSLRGARAIHETYGVQVVGIPGTIDNNIAGTTSLGF
                     HSAIALANQSIESLKATSAAMGSIFFVEVMGAGSGHLALACAYQARAEGILVNEHPDP
                     DTYIEDIVLGTLQRTLGVPNKSHIFVVAERTPHRHHREGGVHGLVDYVAQTIATWPQR
                     RDATGPYSLASATKATILGHTLRGAPPTPEDKMIAQHLAYETVRRLVEQPDTIVGCMV
                     AYHDSNRIETIPLHAVSPKPFDWELFTRMHVTELQHDRV"
     gene            28997..29458
                     /locus_tag="PK589_10865"
     CDS             28997..29458
                     /locus_tag="PK589_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HNJ20146.1"
                     /translation="MSVSRVTRLVLWVCATLLLHLGVAFTADPGPALLHTPVPHLTPA
                     PPQTPPADDGAVRAGPGSKQGEVRDTIPSAARDTLKAIEARHGEPLPGYVGGRNFLNR
                     ERVLPRGRYREYDVHPKVPGKNRGAERIVINQFSGKAYYTADHYRSFVPMN"
     gene            29465..30001
                     /locus_tag="PK589_10870"
     CDS             29465..30001
                     /locus_tag="PK589_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HNJ20147.1"
                     /translation="MSITALQSIKKPWAHLLVHAEGEALDKLLSVPAQFVTKTVSGKK
                     CKTKAGLLDEFARVFSFPDYFGHNWDALEECLADLDWLPGKGYLVVVTDADQVLTKPD
                     EEDDFETFVEILSEAGEAWSLKESDDATGNGLPFHAVLAVQERHKRTRHNWFAPPLAM
                     ERKATKSGGSKGSKTSGR"
     gene            30034..30444
                     /locus_tag="PK589_10875"
     CDS             30034..30444
                     /locus_tag="PK589_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HNJ20148.1"
                     /translation="MGLFDQLGQAATGMLGQTGNNNPLIQAVVGLLAQNSHVGGLGGL
                     IQAFQKNGLGDIVNSWVGTGKNLPISPEQIHQGLGGDLLKQLASQAGLSTEAAGGQLA
                     NLLPGLIDKLTPGGKIPDSTLIEQGLTLLRGKLG"
     gene            complement(30487..30738)
                     /locus_tag="PK589_10880"
     CDS             complement(30487..30738)
                     /locus_tag="PK589_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20149.1"
                     /translation="MTSVRVLCAWCVRDGKPAFLREKLPLDDLSETHGLCEDHFTSLS
                     RSVKQVVTPQVWLLSRLNDLSWGLTRWIQRQMGRLLFLS"
     gene            complement(30935..31168)
                     /locus_tag="PK589_10885"
     CDS             complement(30935..31168)
                     /locus_tag="PK589_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20150.1"
                     /translation="MMKVICSWCESEGRPAVVREKAPLADGRETHGICASHLQQMGIG
                     RDPLVPCYEEGSRQRYEPAVIRRRSEVASALLQ"
     gene            31415..32698
                     /gene="hemL"
                     /locus_tag="PK589_10890"
     CDS             31415..32698
                     /gene="hemL"
                     /locus_tag="PK589_10890"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNJ20151.1"
                     /translation="MKTQRSEQLFAEAQQIIPGGVNSPVRAFRSVGGQPRFIARAKGA
                     RLYDVDGNSYLDYVLSWGPMILGHAPATVTKAIQQAAANGTSYGAPTELEIQLATMIR
                     EALPSMEQVRLVSSGTEAVMSALRVARAYTKRDGILKFEGCYHGHSDYLLAKAGSGLT
                     TLGIPDCPGVPEDFTKHTLTAPYNDLVTAEKLIKKHHRQLACVIVEPIAGNMGVVPPS
                     PEFLEGLRKLTDAHGMLLIFDEVISGFRVQYGGAQTLYGIKPDLTILGKIIGGGLPVG
                     AYGGAKDIMKMIAPSGPVYQAGTLSGNPLAVTAGIETLKRLKKPGTYEQLEQRSVALS
                     EGLGKIASKAGVPLTQTRVASMMCAFFTQGPVVDWATAKKSDTKVYAKFFHHMLDAGV
                     YLAPSQFEAAFMSVAHTPRDIERTLNAAQAAFKTL"
     gene            complement(32823..33056)
                     /locus_tag="PK589_10895"
     CDS             complement(32823..33056)
                     /locus_tag="PK589_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20152.1"
                     /translation="MDMFGKVGLVEVPILIAPDQKAWMDRMIKEGKIAIPPGGTLEKG
                     SLYSMFVRMLLHNAMEEQKRQEALDMDDEDDDE"
     gene            33274..33903
                     /locus_tag="PK589_10900"
     CDS             33274..33903
                     /locus_tag="PK589_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNJ20153.1"
                     /translation="MRCTIVPDSRFLSRFALLLLVASQTTLAHGAVEESLSTLHTYTP
                     AIRNDPSPFHCTDCWDLKPDQRPSLPQHQSQRYRRGHHERGLRPHKNPFAKKSHSFSR
                     GMRSLPRHALSMGGFERTVEGWQVRTVDGDTIRYGTDRIRIRGYNAPELSEPGGREAA
                     LRLEQLLHDGIINIIPHGHDVYGRTLADVFVNGHNVADMMTLEGYGRRG"
     gene            34090..34551
                     /locus_tag="PK589_10905"
     CDS             34090..34551
                     /locus_tag="PK589_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20154.1"
                     /translation="MPNRLHSILLLTGLFALTASAWGAVPAGNTLDIVIGNGPHAGTY
                     KPPAETVICLHAKTQNRYSAAWKDFDAHDAKAIAEAGISVSNPDGAGAKQGDVRVAFG
                     DPDKKPTVYSIDQAPLTMTKNGKGAEITFQGKTKDGIQLRVTAKCSDVEEM"
     gene            34601..35176
                     /locus_tag="PK589_10910"
     CDS             34601..35176
                     /locus_tag="PK589_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNJ20155.1"
                     /translation="MKTQYYTATSLDGFIATEDDSLDWLFPLGNVNETSYPSFIAEVG
                     ALAMGAATYEWMLRHTDKIADQQTGAPWPYSQPAWVFSTRPLPMIPNADIRLVRGDVR
                     TVHTDMRQAAGSKNIWIVGGGDLAGQFYDAGLLDEIIVQVGSVTLGRGKPLFPRRVTN
                     PSLRLVSVHRIGPGFAELRYEVPKEKQADQA"
     gene            35272..35685
                     /locus_tag="PK589_10915"
     CDS             35272..35685
                     /locus_tag="PK589_10915"
                     /inference="COORDINATES: protein motif:HMM:NF018826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HNJ20156.1"
                     /translation="MNAKDIAELAKATMNGSMPFPEIVGKLIANGVEYYHVDYALSSF
                     SFYTGSGTVVTAPLVFEGLPSISEHFDTAALKAAILDSQQHGQKFRAFCERAVKAGVQ
                     GYFAFLRGKRVTYFGRQGDQHTEWFTGARPNEASQ"
     gene            35725..36015
                     /locus_tag="PK589_10920"
     CDS             35725..36015
                     /locus_tag="PK589_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20157.1"
                     /translation="MKPLLASLFLVALLPGVTDTKATEKRPLASHRSGVEPSKTGECS
                     TDHPIKGNFTTRSGERCIYHMPGQRWYQRTKAERCYATEAEAVKDGCRRSKV"
     gene            36168..36614
                     /locus_tag="PK589_10925"
     CDS             36168..36614
                     /locus_tag="PK589_10925"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNJ20158.1"
                     /translation="MTSSMRLFVLNSMMLSALLTACVSTPSPDAMKHIAERNRWELAH
                     WGNQAIPYEDDGEAVILAFKDGKVSGQAGCNRYAAAITFGPKTGEVSVSHGITTRMAC
                     QPKRMEFEAAFIRAFEASTRYRLDGESLLFESAIAQPLEFYRRPPE"
     gene            36832..37341
                     /locus_tag="PK589_10930"
     CDS             36832..37341
                     /locus_tag="PK589_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20159.1"
                     /translation="MAGKIFSALLASGFPNSIRLCGKVMVSEENADARSMSQPPEGRA
                     SGRPAKGNVVALDRLFPFLYGGVLAASLWWIWMTLGRPFHPGWIAAPLLVTTIADWLE
                     GLMLLAQLRHYMSPNQQYAESLLIRLSGCATIIKLWLTSGLYVTLAGLVLNLLFTFPG
                     RRLASDAAE"
     gene            37381..38691
                     /locus_tag="PK589_10935"
     CDS             37381..38691
                     /locus_tag="PK589_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNJ20160.1"
                     /translation="MTSLEAFTSHPTVEAAVRDAQTATEGKHLSDIVDDHGFQYVDFV
                     MEGGGVLGIALTGYTYVLEQAGIRFLGIGGTSAGSINALMIAALGPPEEAKSERLLHI
                     LAGMPMASFIDGDQDARDFSQSALGKVGMMKLLLKAMQVVDNLSDDLGLNPGTAFYDW
                     LTAELLKVGVRTNADLAKRLQTVPAGLHLRPDRTDRQLTEADTIGRLIMVAADITTET
                     KVEFPRMAELYWAKPDEVNPAYFARASMSIPLFFHPFRVNNCSQDRAKWKDVASYDGV
                     PPDTVMFMDGGIMSNFPINLFHQPYHVPASPTFGAKIGTDRAQPAVIDKPSQLLGAVF
                     DAARHTLDFDFISRNPDYKHLVAMIDTGDHNWLNFSMEDTDKIDLFARGAAAAAEFLQ
                     TFNWTKYKSIRKGIADAFLASNTNNGGSLKLAGNSQRLVALSSH"
     gene            38847..>39558
                     /locus_tag="PK589_10940"
     CDS             38847..>39558
                     /locus_tag="PK589_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNJ20161.1"
                     /translation="MHRNTLSVSFLPALLFGALQLFLEPTAGSAEQIRAAMSAPQPEA
                     WWMERHERTVARIRQGHVDLLFIGDSITQGWEEDGRPVWNAFYQHRHAANLGYNGDQT
                     DNVLWRLQNGELDGITPKLAVVMIGTNNATRREDPPEETAAGIQAILTTLRTRLPDTK
                     ILLLAVFPRGLSADDRLRRVNQAVNERLPAFADQRQVFFLNLNRRFLDDNGRLPEEMM
                     PDALHPGERGYRVWAEGME"
     assembly_gap    39559..39658
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(39895..40296)
                     /locus_tag="PK589_10945"
     CDS             complement(39895..40296)
                     /locus_tag="PK589_10945"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HNJ20162.1"
                     /translation="MDIRIYYEDTDCGGVVYYANYLKYFERARTHYLEERGLSVAGLR
                     DQGTQFVVVHAELDYRSPGRYGDTLVIDTTLTDASQASFTFAHVLRERTSGRIVVEGS
                     AKLVTVDEQLKPKRLDKLTLAALQGPPHTRH"
     gene            complement(40737..>41432)
                     /locus_tag="PK589_10950"
     CDS             complement(40737..>41432)
                     /locus_tag="PK589_10950"
                     /inference="COORDINATES: protein motif:HMM:NF021207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HNJ20163.1"
                     /translation="GGQKPLYRALAELALGYGLWEQFQYRQAWEKLKTSLKALDMASI
                     WGGPPGLKALLPLIKANSGFLEKMVLDPAEVKEGVVLDLLAHAHRRAHGDHDPERAMV
                     ALVRALEACAQRQLFKQYKIKTWDVQPDQLPEALRDTCRTSYLDDVDGKYKLPLQSQW
                     RTLAGLGDQMGQAFLRDWPKMKPLLDAANHAVLGHGFEAVKGERVQQLSDMVMKLTGV
                     SESSLPKFPVLSL"
BASE COUNT         9144 a        12759 c        11393 g         8036 t
ORIGIN      
        1 ccatatttcc gccggtcaca aaggcccgcg gatggccggg acgatcggcg atgttctcgg
       61 tcccgatgtc gtaggacagt gccagcgtga gatcgtcgcc tttccattcc acgatatggt
      121 tcgcgtaaaa ccgccagaac tgcagcgcgg tattgctttg atccggccca ccatagagag
      181 tttgcgtgag cgtcagccga ggtgtggcct tgtaggccca ttgaccgcca taactgggct
      241 gatcgttggg atgcgagaga tggtaatagc cgttgatcac aaacgcggtg atggtcaact
      301 catcgctgac gggatatttg gcattgacgc cgaacatcat gtagggcgta ttgtccgcaa
      361 tccaggaacg cgtgtagttc gcattgtctt tcgcatacaa cgactcatag ccgataagac
      421 tgttgaataa cccggcggtg atggtaagcc ccttgccgat cggtgccaga taggacacat
      481 tcgcgcgatg gatgtgccgc aaggtatcgg ccccgctgac ttcccgttcc ccctgcaaaa
      541 atgcaaagcg ctcagagtcg taccctcctt gcacgccgag ttccatgccc caccgtgaag
      601 attcattcac atctttgcga acataggcga gggccatgtt cggcgcgaac tcattatgcc
      661 gcgccgccgt ggcgcggttt cgccagagat ggttttccgg aaaattgaaa ttcagaatgt
      721 aggcaacatc gagatacgcg ccgtagtgcc agagcccgct atcggcgctg acagccggac
      781 tatcaccggg cgtcgaactt ggttcggcgg catacgccgg ggcgatcaac aaccaaaaga
      841 acagcaccca cccgagccga ctcatcccgg ccgaaaagat aggcctcaca gccagaccga
      901 tgtcgttgcg caacggcaga ataggttcca tctcatgctc atgatccaac aaacttcgcg
      961 ccctccggaa atcgactcta cggcatttcg tatcaaaaca gcattaaaac cgatcatgga
     1021 gatgtaagag atccgtaaaa acggccggaa tactcattcc gcccggagtc gatagccgac
     1081 ccccagttcc gtcacgagat gacgaggctg agcggggtcg gcctcgatct tattcctcaa
     1141 ttgacgcata tacacacgca catagtgtcc ttcgtctaca tggaggggcc cccacacttc
     1201 tttgagcaac tggcgatgtg tcacgacctt cccggcatac cgcacgagcg tcgtgagcaa
     1261 cttatattca atcggcgtga ggtggacttc cgtccctgag acaaacactt gccttcgccc
     1321 gagatcgacg gtcacctcgc cgaacgtaca taccggctca accgcgtcac cgatcgggcg
     1381 atgggcatgc cgcaacgccg cgcgcatacg ggccagcaat tcatccacgc caaacggttt
     1441 cgtgacgtaa tcatcggcgc caagatcgaa ggcctgcacc ttgacctgtt cctggtctcg
     1501 tgcggagagc acgatgatcg gcatgttcga ccaggcacga attttctcga tcactgcggt
     1561 gccgttcatg tcgggaagac cgagatcgag gagaatgatg tcaggatttc gagcggagat
     1621 ttgcgccaac ccatccgcac cggtggtcgc ttcgtacaat cggtaaccat aaggcgggag
     1681 agtcgtgcga agaaatcgtc gaatttctgc ttcatcctcg atgagcagga cggttacctc
     1741 atgggtcgtc gtggatggca tggatgacag atcttaaatg gccgcgctgt cgtgctggtt
     1801 cgcttctggc tcgacggtcg gctgactttt ttctgccggc agcgtaaagc ggaaactcga
     1861 tccgccaccg gaacgagagt ctacccaaat acgccccccg tgggattcga tgatgccccg
     1921 gcagatcgtc aatcccagtc cgacgcctcc ctcacgctca ggatgaagct gatagaactt
     1981 ttcgaagatc cgccgctctt cgccttgagg aattccggga cctcgatcag caatttctat
     2041 cagaatcacc ccgtccttca ccgacgccgt cacctcaatc ggagaggccg tcggagaata
     2101 tttcgccgca ttctccaaca gattaatcag cacctgttcg atcaacaccc catcgacgag
     2161 caccagggga agattgaccg gaaaggcggt cgtgaccgta tgcgtggcga tccgggattt
     2221 cacccgggcc aatgccgtgc ccaccagctc ctccagcgca tgccattcct tgcgcagatg
     2281 catggctcca gcttccaatc tcgtcatatc gagaagattc ttcagcaacc ggtcgagtcg
     2341 tccggcttca tcgctaatgg aacggaccag gtcctgctga ctctcggtcg ataggggccg
     2401 ggtcgtgctc agtaaggcac tgctcgcgcc cacgatcgtc gccagtggcg tccgaaggtc
     2461 atgagaaaca gaactcagga cggcattgcg catgcgctcc gtttccgtct gcacgtgagc
     2521 ttcttgagct tcttcggcta atcgcgcacg ttccagcgcc aacgccatct gatttgcgaa
     2581 ggtctccagg agatgcagtt gttccggagc gagcagttcg gcaggtcgag acgatcggac
     2641 cgcaaggact cccacggccc catgagaccc caacaacggc agatacagcg aatcagatcc
     2701 ggacagggta ttggtgccgt gaccggcgga ttctttatgg tcgaacaccc attgcgagat
     2761 gccggcttcc ttcggatcca gctcgaaatg cagcgcttca ccccgatgaa gatgcacccg
     2821 cccttcccga tcaggaagga acacggccac ctgacaatga aagacgtcgt ggatatggcg
     2881 ggacgccact gaagccagtt tgtccacccc acgttgagtg atcaattccc tgctcatggc
     2941 atagagtgcg gcggttcgct tctcacgaat caaggccgcc tcggcctgct ggctcttgtg
     3001 ggcagcgagt cggctgatca gcagcgctac tagaagcatg actgcaaatg tcagcagata
     3061 ctgcgcgtcc gatacggcga atgaatagta gggcgggatg aaaaagaaat cgaaggccgc
     3121 gacgctcatc agtgaagcca gcgctgaggg gcctcggccc catctggtgg caacgaccac
     3181 caccccaaac agatacacca taatgagatt agcctgcccg aagtatgggg acatgaccag
     3241 actcaatgcc gtgcaggctc ccacaaccgc ggaggcgtag ccgtagttcc ggcgctcact
     3301 ggtccgttga agcaatcctt tcacaagagg tttgccagta cccccctcac cggtcatcac
     3361 atagatatcg atgtcgccgc tcccgcgaac gagatcatcc acaaccgaac cgaacagcca
     3421 ttccttccag cgcgaacgca atggttttcc gacaataatc ttggtggcat tgcggcttct
     3481 ggcatacgcc agtaactgcg cactcacgtc ctcccccgtc aacgtcaccg tttctgcgcc
     3541 catccgctcg gccaaccgca aggtgtcgac accttgattc cgctcctgat cagacaaccg
     3601 cagatgcttt gaggtctgga cgtagacagc gatccacctg gcatggaggc ccgtggccat
     3661 cttcctggcc gcgcgaacca gcatcggccc acggggtttc agattcacgc aaaccaagat
     3721 ggtttccgct gccggccagg tgccaacgac cgcatggtcc cgacgataca cttccatttg
     3781 ttgatcgaca cgctcggccg tccgacgcaa ggccatctcc cgcaacgcga tcaaattacc
     3841 cttccgaaaa aaatgcttga tggcgtgctg tgcctgatcc ggcagataga ccttgccgtc
     3901 cttgagccgt tggaggagat cgtccggagg gagatctacc aattcgacgt cgtcagcctg
     3961 ctcaagaacg gcatccggca cggtttcact cacacgaatg ccggtgattt gagctactgc
     4021 gtcgttgagg ctttcgatgt gctggacgtt cacggtggta tagacgtgaa cgcctgcttg
     4081 aaggagttcc ctcacatcct gccaccgctt gggatgtctg gaaccggggg cattcgtatg
     4141 cgccagttcg tccattaaga ggagctgcgg ccggcgcgcc agagcggcgt cgagatcgaa
     4201 atcccgaagc cttgatcccc cgtagaccac ctcccgggga ggcagagtcg gcatcccatg
     4261 gacgagcgct tccgtttcgg cccggccatg agtctcaacc cacccgatca ccacatcgat
     4321 gccgtcccgt tgctgctcat gggctgcctg cagcatggcg taggttttcc cgacccctgc
     4381 tgtcgcgccg aaaaagacct tcagtctgcc gcgcgtctga tcggcttctt cagcctgcac
     4441 gcgcctcagc agtagatcgg gatccggacg ctggttctcc atggcactcc tggacaaaga
     4501 aattcgatgg gggacgaaaa ggccacaaga ttggcagagc aaattctaga agacagacgg
     4561 aaaggcgtca acgagcctgg ccggatgcat ccaatgcgag gttcaacgcc agcacattga
     4621 cccggcgttc accgagcagt cccagctgac gcccttccgt atgttggatg accagtgtcc
     4681 tcacgactgc ttcgtctttt cctcgtgcac gagcgacccg gcgcatttgg tactccgccg
     4741 cggcggggct gatatgcggg tcaaggccgc ttcccgacgc ggtcaccaga tcgaccggga
     4801 ccggtgcatc attgcggggg tccgcagcct tcaacgcagc caccctcgtc ttgaccgcct
     4861 ccgtcagcgc gggattggta gggcccagat tcgacccgga ggaggcggag gcattatagg
     4921 ggaacggagc ggtcgcggaa gggcgtcccc agaaatactt gggatcgtcg aagggttggc
     4981 cgatcagcgt ggacccgatc ggcttgccgt ctttatagat cagactgcca ttggcctgat
     5041 gggggaacag cagctgcgcg atccctgtca cggccagcgg ataggccaca ccggtcagaa
     5101 tcgtcaggat gaaaaacacc atcagcgcgg atcgaagttg agcggccatc gtgatctcct
     5161 cagaccagtc ccagggcgac caggagcagg tctatcagtt tgattccaat gaacggaaca
     5221 atgatgcccc ccagtccgta caccagcaca ttgtggcgca gcagcaagga ggcctcaatc
     5281 ggccggaact tgacccctcg cagcgcgagc ggaatcagcg cgatgatgat taaggcattg
     5341 aaaatcaccg ccgacaagac ggcgctttcc ggcgttgcca gtcccatgac attcagcgcg
     5401 cccagggccg gatacgtcgt ggcaaacgcg gcgggaataa tcgcaaaata cttcgcgaca
     5461 tcgttggcga tactgaaggt cgtaagggcc ccccgcgtca tgaggagctg cttgccgatt
     5521 tccacgatct cgatcaattt ggtagggttg gaatccagat cgacaaggtt ccccgcttct
     5581 ttggcggcct gggtgccggt attcatggcg acggccacat cggcctgcgc cagggccggc
     5641 gcgtcattgg tgccgtcgcc cgtcatggcc acgagtcggc ctcctgcctg aagatcacgg
     5701 atgagtttca atttggcttc gggagtggcc tgcgcgagaa aatcatccac gccggcctct
     5761 gcggcgattg cggccgccgt tttctggttg tcgccggtga tcatcatcgt tttgatgccc
     5821 atgcggcgca actcggcaaa ccgttccttg atgcctccct tgaccacgtc cttcaaatga
     5881 atcacgccca gcactgtttt gccttcggcc acgacgagag gagtgccccc ttgcatggca
     5941 atcgtctcga ctgcatcgcg cacggcggga ggaaacgagc cgccttgttg ggtcacgtag
     6001 tcttcgatgg cctccgccgc gcccttccta atttgtcggt cgtccagatc gacgccgctc
     6061 attttcgttt gcgccgtgaa cgggaggaag gtggcgccca tcgcatggat atcgcgccct
     6121 ctgataccat agcgttcctt tgccagaatc acgatgctgc ggccttcggg cgtctcatcg
     6181 gctagcgagg ccagctgcgc cgcatcggcc acagacgttt ccggcacgcc ttcggctgga
     6241 ataaatgccg tggcctgccg gttgcccagc gtgatcgttc ccgtcttgtc gaggagtaac
     6301 acgtccacat cgcccgccgc ttcgacggct ttccccgaca tggcgatgac gttcgcctgg
     6361 accatgcggt ccatgcccgc gatgccaatg gcggataaga gagcgccgat cgtcgtcggt
     6421 atgagacaaa ccagcagggc caccagcacc gtgaccgtaa tcggcgcccc ctgccccgta
     6481 gcatgcacgg aatagagaga aaacggcagc aaggtcaccg tcgccagcag aaagacgacg
     6541 gtgagggccg ccaagagaat attgagcgcg atttcattcg gcgtcttctg gcgcctggca
     6601 ccctcgacca tggcgatcat acgatcgaga aatgtttcac cggcgctggt ggtaattcgc
     6661 acgacaagcc aatctgacag cacccgcgtg ccgccggtca cggcactccg gtcaccggac
     6721 tccctgatca ccggcgcgct ctcgccggtg atcgcacttt cattgacgga tgcgatcccc
     6781 tcgatcacat ctccatcgct gggaatgtaa tcccccgcct cgaccagcac gatgtcacct
     6841 ttcttcagca gactggctgg aacgagactg tagacctctc ccggtttgcc gatggcggca
     6901 ggcgccgcca ggtagtctcc cccggaggca ggtgttccaa atcgcttggc ggtcaactcc
     6961 gtcctcgatt tgcggagcga atcggcttgg gcctttcctc ttccttcagc aatcgcttct
     7021 gaaaaattgg cgaacagcac ggtgaaccag agccacagcg agatggcgag gataaaccag
     7081 gtcggtgctt cgcccgcccc gaacaacgcc tggaagaaca gcagcgacgt caagaggctt
     7141 cccacgaaga ccacgaacat gaccggattg cggacctgaa tgcgaggatg gagctttcgt
     7201 agcgcatcca gcagggcctg tcgaacaagc ggcggatcga agagcggtct ggctttcgtc
     7261 gatgtcgtca tggaaatccc tctgctcgtt caggctagcg agcgaccatc agcaaatgtt
     7321 ccaccaccgg ccccaacgcc agggccggaa cgaaggtcag ggcgccgacc atgatgacga
     7381 cccctatcag caagaccacg aagagcggcg tatgagtcgg gagcgtgccc aaccccagcg
     7441 gcaccatctt tttgcgcgcc aacgatccgg cgagcgccaa tgtcggaatc gccaaccaga
     7501 atctggagac cagcatggcc aatgcgcccg tcacgttgta gaagggggta ttggcattca
     7561 gtcccccgaa ggcactcccg ttgttattgc cctgggaggt ataggcatac agaatttcac
     7621 tgaacccatg tgcgccggca ttgagaatgg acaacgtgcc ggactcagtc acgaccgcca
     7681 ctgccgtgaa ccccaggaca acgatcggca tgatgaggat caacagcgcg gccatcttca
     7741 tttcataggc ctcgatcttt ttccccaggt actccggggt ccgtcctacc atcagaccgg
     7801 cgatgaacac cgcgataatg gcgaaaacca gcattccgta gaggccggac cctaccccgc
     7861 ccagaatgac ctcgccgaat tgcatcatga gcagcgggat caaaccgccg agtggcgtaa
     7921 aggaatcgtg catggaattc accgccccgg tggacgtggc tgtcgttacg gtggcaaaaa
     7981 gcgccgaacg agcaactccg aagcgagttt ccttgccttc catgttgcca ccggactgcg
     8041 ctatctggac gttctgctcg acacccaacg cagtcaggcg agggtttcct gcggactccg
     8101 cccagtaggc ccccagcacg aagaaggcca atacgctcag catcgcagcg aggatggtcc
     8161 atccctgcct agtgtcgccg accatcattc caaacgtata ggtcaacgat gcggcgatca
     8221 gggtttcagc caggatcaga atgaaatcgg tgagcggcgt cgggctttca tagggatggg
     8281 ccgcattggc attaaagaat ccgccaccgt tggtccccag atgtttgata gccacttggg
     8341 acgctgccgg accgacggcc aagacttgct caatcccggt tgttgattcc attttggcaa
     8401 cgcccttctc gtccactacc ggctgacctg cggcatcgac gacgggcttg tcataggtca
     8461 caggctggag gagagcggtt tggtgataca acccgaaggt ctggaccgtc ccttgcgaga
     8521 cgagaaccag cgcgagaacg gctgacagcg gcagcaagat gtacaacgtg ctgcgagtca
     8581 ggtccaccca gaaattgccg atggtcgagg ccgtccgagc ggttaacccg cggatgagcg
     8641 cgaccagcac ggccatgccc gtgccggcgg agacgaaatt ctgaactgtc aggccgagca
     8701 tttgcgtgag ataactgagt gtcgattcac cggcataggc ctgccagttc gtattggtaa
     8761 tgaagctgac ggcagtattg aatgcgaggt ctggcgccac cgccccgaaa gccgacggat
     8821 tgagcggcag caatccctgc agtcgctgca aggcatagag tgcgaccaat ccggcgacat
     8881 tgaacaacaa catggccacc gcatagctcg tccagttcat ggcctcggct tcgcgcacgc
     8941 cacagacacg gtagatcagg cgttcgaagg gtcccatcac ccgatccatt ccgcaggcct
     9001 ggcccgtgta gacacgcgcc atataccagc ccagcggttt aaccaacgcc gccaaaacga
     9061 gaaagaacag tccgatttga gccaacccgt tgatcgtcat tagaaccact ccgctttcag
     9121 cagcgcgacc atgaggtaca ccaacaaacc cagcgacaac actgcgccga ggacatacat
     9181 cgcattcatg accagacccc ctttagagtc ggtccaacgc cgagataagc cagccggaca
     9241 gcagaaaaaa cacagcagcg atcgctaaat agatgagatc catgtcaatg ttccttaatg
     9301 agacggcaca cgtattgagg cgcgcttcgc cggagtctct ttccaagccg tcgccaacca
     9361 gccacatcgc tgcacgcccc ttctcttcca ccacatgttc aaggtagcgc gcagaaagta
     9421 aaaacggtgt aaaaaagcga aggaaatgga tttctgagaa gatcttcccc aacagctgtt
     9481 cacgggttcg gaggagatcg ctacagacga ctgatcactc gtaatacatc cacttacgag
     9541 cggggtgcta aggaagacat gtggacaaag ccgtgaccgt ctattgatca ggtcacagag
     9601 aattgttgga gcaactagag aggcgtgact gcatcaggac actactgatc tgaatccggg
     9661 caatctttac gtagctcagc caacaccgcc agaatttcga ccggccgtgg aggacaaccg
     9721 gcaatacgga catcgaccgg aatgtggcga tctaccggac ccgtcaccgc ataactcccc
     9781 ttgaacattc cgcaagtgat cgcgcaatca cccagcgcaa tgacgagctt cggatcgggc
     9841 gtctgcttat acacatcctt taaggcgcgc tccatgttga cggtcaccgg ccctgtcacc
     9901 accagcgcat cagcgtgtcg cggcgacgca gcgatctcca ccccgaagcg ctccacgtca
     9961 tagacgggat tggccagcgc attcatttcc atctcacagg cgttgcacga cccggtatcc
    10021 acctctcgaa tcgtgaggga tcgcctgaat ggcttgacct tttcaatcgc ctcccggctc
    10081 accggctctt gtgacgcctt cgccatagga tattggcccg tgacgacgcc agtcgtaaga
    10141 cttttcttaa tgattcgaaa catggcacct ccgtgagatc gtcgggacaa tcgcgcagtc
    10201 agtcgccttc agcttcccgc tacagatcat tgccggcata ggacaaattg aagctcttat
    10261 tgatcaacgg aaaatccggc acgatgttcc ccagcgccgc ccactgaatt gccggccaat
    10321 gcacaaacga tggatcccgc actttacacc ggtgaatccg tccctcttcc cctgccatca
    10381 cgacatacag aatttcacca cgccaccctt cgacggcaga tatcgcccat tgcccctgcg
    10441 tcacaggctc gaccgcctgg gtggccagcg gccccatcgg cagttgtcgg cgaagcgtat
    10501 ggatgagacg catcgaatca tgcacctcat ccaaccgcac ccgcatgcgc gcgcggacat
    10561 ccccgtagcg atacaccgcg accttcggtt gacactcggg atacgcggcg aacggccgat
    10621 cccgacgcaa atcccgatcg ataccggaag cgcggcccac gactcccatg accgcatgat
    10681 cccacgcgat actttccgac aacactcccg tgttctccaa tcgttccgtc agagaggcat
    10741 tcgccagcag aatcttttca attccggaaa actcctgttc aatggcttgc aattctgcgt
    10801 cgacctgcga cagttgcaca ccggaaagat ctatcgacac tcctccgacc ctgttcaccc
    10861 ctcgaagaaa gcgtgacccg gtcaggccat cattcaactg catgagctgt tccttcagac
    10921 ggccacagtg cgcatgtgcc agcgcatagg ccgtatcgtt gcagatggcg ccgacatcgc
    10981 cgatgtggtt gtgcaaccgt tccagctcca gaaagagact ccgcagatag cgggctcgtg
    11041 gcggcaccgt cattcccaag agtgtttcca ccgcctgaca gtaggcgagg ctatggccga
    11101 ccgaggtatc gccggagacg cgctccgcaa gtggcacagc ctccgtgagg cgctgttgct
    11161 cgaatagttt ttccaccccg cggtgcttcc agaaatgacg cacttcgagc tggaggatcg
    11221 gttccccggc taccgaaaac cggaaatgac ccggttcaat aatgcctgcg tggatgggac
    11281 cgacaggaat ttcgaagacg ccatcgccgt ggattttcct gaaggcgtat tccccctgcg
    11341 cacgctccag cacctgatcc caggcaaatt ctttcttcag cggatgcatt cccttcggcc
    11401 aatgttcatg acgcaccagc ctccgcatgt ccggatggcc cacgggaatc aagccgaaga
    11461 gatcacggat ctcccgttca taccacttcg ccgcatggac catcggggtg atcgacgcaa
    11521 actcccgctc ctcttctgcg agatcaaccg tcaggagcaa ccagtctttg cgtgctgcca
    11581 gcgtgaatag gtaccagagt tcatcgcgag cgtcgcgagg gcgatgatcg atggcccaga
    11641 ggagcgaaag cgagccgcgc aattccggat cggtatggat atagcgtgcg acggcgggta
    11701 cgtctggctt ccggagacga agcatgggca cgccctgcat cgaaccgact tggatgatgc
    11761 cctgcggaaa ggcctcctgc actcgcgcgg ctgctgtcag tccgtccggc atagggttca
    11821 cctcaccaca atgacgtgga cagctctagt caccaatgtc cgcaacgatt cgggaagcat
    11881 gacgctcaag ccgagcaacg ccacgatcat ggccaacatc ggtacatggc cgactgtcca
    11941 gggctctcct cggcggatct cgtcctgcgg agtgccccag accatcgcgc caatgcgcgc
    12001 catcatgcca ccgaacagga ccacgccgaa aaacaaaaag acgccgacga gtccaagact
    12061 ccgcatttca tcggaaaccg tcaccgccac gaaccgaccg acatgcatcg tatcggatgc
    12121 aaaattctgt gccgagagcg ccgataccac gagcaattca ctcacgaatg gcgaaaacgg
    12181 cggcaggccg accaacgcga acccggctac aagagtcgcc agtgccgtga tcggttgcga
    12241 tcgcccaagc cctcgtacat ggtcgatctc caacgagtcg taccgccgat ggatgttgcc
    12301 cgcgacaaaa aatgccagcg ccttggctaa cgcatgattg agcaaatgga acaggcctcc
    12361 gaaggtaccg accaacccgc ctactccaaa accgagcatg gccagcccca tgtgctcgat
    12421 gctcgaatag gcaaacagcc gcttataatt gtgctgtagg agaatgaaca gcgaggccac
    12481 cacaaacgac acgaacccga acgtgagcag cagattaccg gtgaactgag gcgggagtgc
    12541 ctgatcgacg agcgccttgc tgcgcagcac cgtatacacc gctaccgttt ccaacacccc
    12601 ggccaacatc gccgcaaccg gcgccggcgc ttccgtatag gcgtcgggca accaggtatg
    12661 catgggcacc aagccgacct tcgtcccgta ccccacgaga atgaagagaa acgccagctt
    12721 caggacattc ggatccaatc gatccgccac cccgatcagg gctgtcacgt tcaacgccga
    12781 actggcatcg ccaagcaccc gcaccgagga gtaataggtc agcacgaccc cgaacagggc
    12841 aagggcgatc cccactgagc agagaatcaa atatttccag ccggcttcga gtgattcccg
    12901 gcgtcgaaaa aaggcgatca gaaaggtcgt cgccaatgtg gtcccctcca cggcgaccca
    12961 ctgcacgcct aagctgttgg ccatcgtcgc ggcaaccatg gcgaacagaa acatgtgaaa
    13021 caggaagaag aaaaccccca ggtgcttcgg cgcgatcaca gcacgagcga cctgctcatc
    13081 catatacgac cacatgtaca gcgaacagga gagtccgatg gccgtgatga tgatgagaat
    13141 aaagtccgac aacgcatcga tatacacgag cgagcccaac gtcgtaaccg atccttccgc
    13201 caacacccgt ttggtcagga aggtctcggc gacggctaat cccagcatgg tcgtaaaatt
    13261 gacggcgtgc agcagccgcg cacgttggat caccaggctc agcagcccgg ccaagaccgg
    13321 ccctgccaac aacacgatca ccggccacat gggacacctg ttcgcgagtt ctccatcatc
    13381 ggatcatcat tccttcaaga ccgttaactc gctggtatcc acgctatcga acgcatcctg
    13441 cagccgatgg gtgtaaatgc ccgcaatcag gcccgcgacg agtacatcaa aaaatacgcc
    13501 cagctcgacg atcaacggca tgccgtaggc cgccgccgtc gttcccagga agacgccgtt
    13561 ctccatcacc agaaatccga ccatttgcgt cacggctttc ttcctggcga tcatcgtaaa
    13621 caggccgatc aaaatgatgg caagggccag ggccagcgaa tcgcgagtca ggagataccc
    13681 cagcggaata ataggctgcg tgatgaaaaa ggccaccacc accaatccgc cgcagatcaa
    13741 cagcccggcc ggaatgttga tattcatgac cagttctcgc gacacattca gacgctcgat
    13801 gacctttcgg agaatccgcg gcaacacgat cactttgatg accacggtca gtgccgcggc
    13861 tagatagatg tgccgatgcc cggtcaggaa ggcgacgagc gccgccgtgg tcgccaaaaa
    13921 gaacgattgc agcgcgaaca gatcgacgca agccgagagc cgccgctgcg ccacgatggc
    13981 gaagcaactc aacagcagca acaccgagca gagatcgacg agctgcgacc cgagctgtgt
    14041 tgattccgac atcgccttac cctcgcaacg tgtagaagaa caccaaggcc agcagcgcca
    14101 gaatgaaagc ggaaccgagc agttccggca cacgaaacag ccgcaacttc gcgaacatcg
    14161 attcaatcac gccaatgatg acggccaacc ccgttacttt ggcgacatag accgcaacgc
    14221 cagtcgccag ggctgatagt gtcgcctccg tcgcgatccc ccaaggcgcg aacacgttga
    14281 cgagcaacgt caggaacacc agcagcttga tacccgcggc ccattccatc aacgccaggt
    14341 accggcccga atattcgagc agcatggcct catgaatcat cgtcagttcc agatgcgttg
    14401 ccggattgtc caccggcacg cgcccggtct ccgccaaggt cacgataaac atcgcggcca
    14461 gcgccatgag gtgtgcgggc ggcgccagca ccatgccttc cagcaacgcc gactgatgga
    14521 cgatcgtact caaattggtg gatccagccg tcaaggcaat ggccagaatg gacagaatca
    14581 tcgctggttc ggccagggtc accacaatgg cttcccgact gctccccatt ccgccaaacg
    14641 ccgatccggc atccaatccc gccaaaatca agaaaaacgt ccccaaggcc aataggtaca
    14701 ccaacgcaat gatgttgccg gcgaagttga gcggcgtgtg ggagatgaac accggcatca
    14761 ggaggccggc cgccactgtc gatgcaaaca cgatgtaggg cgcggccgta aaaatccacg
    14821 aggtggtgga ggaaatcacc ggttgtttgc cgaacaactt ggccaaatcg gcatagggct
    14881 gcaacagact cgcaccacgg cggcattgaa gacgcgcgtt gaccttccgg atcactccta
    14941 cgatgaacgg agaaaatgcg agcagtatga tgagctgtat gaagacgaat ccgatctggg
    15001 atatcacctg cacgtcgttc ctcatctggt attcactgac gggtccgcag cgtgaacttc
    15061 atgacctgtc tgaccctatc cggcccagag caacaacagc accagcgtca cgaaaatgta
    15121 ggccaagtag agatggagac tgcccgcctg gatgacgcgt agacgctccg cggccgccaa
    15181 cagcccgttg acgatcgggc gatatgcata cttctcgaac aggggttcga tactactgtg
    15241 gaatcgctga tgccggatga agtacttcga ctgctccaga aactctcgct ctaacttcac
    15301 cgtgggctga tagatcgtct caaacacttg tttgatcggc tgcacgaagc cggttgccga
    15361 atattccatg cgtggcgtga gcggaaggcc acaaccccac gtcctggagt accgcgcatg
    15421 cactcctttc ctcagcagcc gggcgatcgc cagccctaac ccagccgcgc cgaccagcaa
    15481 tgccgcgagg accggcatcg aaatactgga gaattccacc gtcactggag caaccaccca
    15541 gccgtctagc gccaacactt tttcgctgat cgccacgccc gccaaggggg tcgtcacccg
    15601 atccaacaag ggaaccacga gcatgggcgc taacccaaac agggcgcaac tcgacgccag
    15661 caggcccatt ccgattcgca tcgacaccgg cacttcaacg gcatgccggg catgggcgct
    15721 gcgcggctgc gccagaaacg ataggccgaa ggcttttgcg aaacaggcca acgccagcgc
    15781 gccggtgagc gcgaggattg cagcggcgat gggcaacatc agcttgagaa acaacgtcgg
    15841 caattgcacg ctgagaaaga ggctctgaaa gaccagccac tcactgacga agccgttggc
    15901 gggcggaagc gccacaatcg acacggcacc aatcaggaaa cagagcccgg tccacggcat
    15961 ccgtctgagc aggccgccgt actcttcgat atttctcgtg tgcgtcacga acagcaacga
    16021 cccggccccg aggaacagga gccctttgaa agccgcgtgg ttgatggtgt ggtagagtcc
    16081 ggccaacaat ccgagtgcgg ccagttcgtg caggccatag gtgtgaaaaa tcatgcctgc
    16141 gccgataccc aacagaatga tcccgacgtt ttctacactg gaaaatgcga gcaggctttt
    16201 caagtcgtgt tccatgagcg cgtacatgac gcccaggagc gcagacaccg cgccgatgct
    16261 caacaccgta aaaccccacc accagggaaa ctgtccctcc aaaaaatcaa agtacacccg
    16321 gattaatcca tagatcgccg tcttgatcat cacgcctgac atcaacgctg agatgtgaga
    16381 cggcgccgcc ggatgcgcgt agggcaacca cacgtgcagc gggacaatac cggccttcgc
    16441 gccgaatccg atcaatgccg tgaaaaacgc gatcgatcgc aggccgtccg gcaaccgctg
    16501 gtctggatgc cgaaacgcct cgaaggagaa ggagccggtt tcttgagaaa agagcagaaa
    16561 cgtcaaaatg ataaaggccg tccccgcatg cgtcatgatc agatagaaca acccggcttc
    16621 acgcacgccg ggccgttcat gctccgtcac caccaggacg tacgagagca gggacatcac
    16681 ttcccagacg atcaggaaga agaacccgtt gtcggccagg accaccagcg tcatggaaag
    16741 cagaaatcca ttgagcagcg agccaagcag gccaatcgac acgcggccgg tgaactcgcg
    16801 cacatagcca tatgcataga tcgaggcagc catcccgatc agtgaaatgg tcaggacgaa
    16861 aaacgccgcc aacggatcga gccgcagcgc aaacgttaag taaggaatgt tggagggaac
    16921 ggaaatcgtc agtggttggg aagccacaag cccgaggagt cccagcgcaa tcccagctcc
    16981 gccggccaaa gcggcagcac tatgcgccag aatgttctgg agatgcgggc gtgatgtgca
    17041 gagtgggagc agccccccga ggaggtaaca gccgagaagg acggtcagga gggtggtcat
    17101 tacgacacta ccctcactgc acgcagcatc gcgatgctca ccgaatcatc aagagacacg
    17161 tcagctcttg cagttgtcaa agtacaagtg gaacgagcag ggtatcacct cttcatttat
    17221 caccttcttg gtcgctgcgc aatgtacccg ccgcgaagga accgcgcagt acgactgact
    17281 tgctaaggag ttgtaggaaa atgtgtcgag gtagagagaa gggagacact gacttcgagc
    17341 gagcagaggc gttatttgcc ggcgtctccg agcacgcacc acaatcgcct cgtgcccgat
    17401 ttctgatcgg tcactgactg accggaaaag aaactctgct gccaggcaac gatctcatcc
    17461 ttgggatggg gtgtggcggt ccaatagatg ccagacttga tattccgaaa gggatgacca
    17521 gccggtagcg agggatcctg ctgatcgaga tcgaccaggg tcttgatttc gtcgatgctc
    17581 ggcgcgcgcc agccctgcgt tccaccaacg gttttcgtcg cgcatcgcgc aacggagcgg
    17641 tcccacacat caaaaatgta atccggctcc cgctcccaga ccaggccgct ggccgaatct
    17701 ttcactgcct ctccgttcag caccaacgta aatcgcgagg ccgcctcttc agcatgaacg
    17761 gcactcaatg acacgccggc ccacaggaag atcagagaag caaacactcg gccgcgcatc
    17821 cacacgatag gcatgaccgg cagtgtgccc gatgtctcgg ggcttttcaa gaacgggagt
    17881 tgcgaatcag cagcgggcta ccagtccagt cgtcgatact gccgtcggag caacgacgcg
    17941 ccccagagca acgacaccag catcagaact gtgccgacgc cataggctag cttcgccaaa
    18001 ccatgatcgt actgcagcca ccccagcatg gtgaggagat tgttccagtc atgcgcgtcc
    18061 acttctttgc cggtaaaccc gccgagcaga atgagatcca ttgcacgagc atcgttgata
    18121 tagggggcga cgtccatgaa gttttgcgcc gtccaccaga gcgccaccga cccgccgaag
    18181 ggatcacgcg tcttcagcag aaatgtgccc aggcagatca gcggcatgag gatctggccc
    18241 agactgccgc cgaggatcat catgaatcgc ccgaagggca tgaagatcag gtgcccggct
    18301 tcatggaacg gaagattgat caggtggagg aaggattcgc cggtgtaatt cgtctcgagc
    18361 ggggtggtga tgaatcgcca gccccacgag accagcaaca gaaagaccag cacacgtccg
    18421 gcaaagtaga atggattgac cgagtcctca gtgataagga gccattcttc aacgacccga
    18481 ggccgagtcg agtcctgtcc cggactgcgg ggcatcaccg gcggagccgg cgagcgatgc
    18541 atcttcgcga aaatgatgcc gcagcgggcg cagtctgcag tgccctcgtc ctgatcccat
    18601 ccgcattttg gacagtgcat caatcagcgc aggatgaagg gttcccaaga gaaggtcacg
    18661 gcaaaaatga acagggcaag ccagccgatc agtacacggg cggttcccaa atcgcggcca
    18721 ggatttcgca ccggcggatg agccaaaccc atcatgccag ccaaccccac ccacaagaac
    18781 caccctttcc agccgagcca accgaagacc atgaggatag gaatcagcgc gaccgccaca
    18841 ctgcgctgac gctctcccag cagggcatag gcgacgtgac ctccgtcgag ttgaccaatc
    18901 ggcaagaggt tcagcgaggt aatgaagagg ccgaaccagg cggcgaaacc gactggatgc
    18961 agaatcacat ccgcggtcgg cgctaagggg ccgatcacga cccaggatgc gaattgtagc
    19021 aacaacggct ctccaaggtg catgccgtaa ttaccttgaa tcggcaccac tgtcgaaagc
    19081 cggagcccga tcaccaaggc gatcaccgcg acgacgaacc cggcaatcgg tccggccacc
    19141 ccgatatcaa agagcgctcg ccgatcggtc agcggagcgc gcatgcgaat gatcgccccg
    19201 aatgtgccca cgaaatgcgg cagccccggc acgaacagcg gcagggaggt cggcacgcca
    19261 tgcagacgcg ataacacata gtggcccagt tcgtgagtga cgagaatgcc tagcagcgtc
    19321 gccgcaaagg gaatacctct ccacaacgag ccggggtcgt cgacaagaaa atcccagggg
    19381 ccgaccaaag gattggtgtt cgtttgatag gcaccggccc agaggacggt aaacagcgtg
    19441 agggcaaaca acatgatggg aagcacgaag gcggaaaatg ccggtcgctc ttcctccacc
    19501 cattcgtctt cctcggcgtt ctcgaacgaa ggggatatca gttcacccga agaccggttc
    19561 tgatccgact cgtctcggtt catgacaggc ctctggtgtt acgtcgcgaa cggattatga
    19621 agacgctctt cgtgaaccgt cgtggccggt ccatgcccgg gaaacaaccg tgtatccgga
    19681 ggaagggcca gcacgcgctg gcggacggaa gcaagatgtg tctgatacag tccggctgga
    19741 ttcgaccggc cgatcgaacc ggcaaacagg gtatcgccaa caaaacacat cggctctcct
    19801 gcctgttcga gacgataaca gaccccgccc ggagtatggc ctggcgtcgg catgaaccgc
    19861 agcctcagcc cgccaacctg cagcgtcccg tcttccttgg gcacgtgcag cacgtcttgc
    19921 gaggggcgcc agctcaagag atcgagatcg tcaggaccga gatagacagg caccggatga
    19981 gccttcagaa tggcgtcgat cccatcggca tggtcggaat gcccatgcgt caggcaaatc
    20041 gcccgaaggc gcaacttccg cttggcgagc gtctccaaca tggcgtcggc gttgtacgca
    20101 gtatcgacga tcaccgtttc accgcgatca tgcacgatat agcctttgac ctcatagcca
    20161 ccgatggaac ccagaatggt gtccacgtga ggggtggagg ccgggagagc ctctggggtc
    20221 cagccttccg ccacctgcgc taactggtct gcgcgaagcc caagcgcacg gcccaccgcc
    20281 tgcacctgct ctcgattgcg cgcaggctgg ccgcgttcca gtccggccag gtccgcctcc
    20341 ggaaaaccgg cggcccgggc cacgtcggct accgatcgct gctgccccgt gcgggccttc
    20401 ttgatgatat cgcttaactc gtcttcaaga ggcataaccg attgtatcca gtttcatgtg
    20461 gattggacat atgaccacgc ggccgtcact gcagaaattg tatcaatctc ttttctcaca
    20521 tcaatgcgcc gagcgcacga cagggcgggg cggctttcac tgcgcgcgtc caacgagggc
    20581 attcttatgc cgcgcgttgc gcgagcaaaa agacgcctcg acctgtctgc gctcccttcc
    20641 tcaactttcc ggcgccagcg taatctcctt cacctcgccg aaactggcca gcactttcga
    20701 caaggcgtct ccggaaccga ccgccagaat ccgcaaccgg tctggattaa aatgcatttt
    20761 cgccgcccgt tggatatctt ccttagtcag cttcaccacc ttatcccgta tctgctggag
    20821 ccagtctttc ggtaagccgt catattcgag atccatcagg cgcgcgacaa tgctggacgc
    20881 gctggtgaac gaaaagacaa acgaattcac gtaggcttct ttggcctcct ccagctccgc
    20941 atccgtcacc ggctcattac gcatccgctc catattcgcc acgaagcgat tcaccacctc
    21001 ctgtgtggac gaaagtttgg tctctgcgcg catcagccat acgccttcat catacacact
    21061 ggctcgcagg ccgctgccga ccgaataggc caacccgcgc ttcgtacgca cgtcgttaaa
    21121 cagccggctg cgaaatgaac tgccgcccag aatgtcgttg gcgatggcca ccgccacgta
    21181 atcgggatgg gtttctttga ccgtcagatg cccgactcgc aaatgggttt gcgacgtatc
    21241 cttgttcacg aatcgcacta ccccggtttt gaactccgac tccgccacag ccggaatcgt
    21301 gacatcaggc acagtccctt tggcccaatc cccgaaggct tcgcgcagca aggccaacat
    21361 gccggccttg tcgaaatcgc cggtcacgcc aagaatgatg ccgttgggat gaacggtctg
    21421 ctgatgaaac gcgaccaggt cttcgcgagt gatggattcg atcgaacgca ccgacgtttc
    21481 acgcgcgctc ggatgggtgg gcccatagag cagtttggcg aactcacggc ccacgatcga
    21541 accgggactg tcctgccgcc gcctgatccc ttccagcgcc tgcaacttcg ccaactccac
    21601 ccggctctgt tcaaacgccg gccggcgcaa caggtcggaa aaaatctgta ctccgcgctt
    21661 caggtctttc ttcaggacat cgagcgaggc agatccggac tccttgctga agccgataga
    21721 aatggtagcc gctaactgtt ccagctcttc atcgacctcg tccggtgaca tcgaggccga
    21781 tccgccggtg cgcatcgctg cgccggtcat ccccgcgagg ccaaccttgt ccgccgggtc
    21841 cagccaactg ccggttttga gcgtcgcgtt gatcgtcacg agcggcaatt catgatcttc
    21901 aaggaggtag accaccatcc cgttgtccag cacgagacgc tccgcgtccg gcggattgaa
    21961 ttccaccgtc gggaatcgca tggaacgagg attgtccaat accaggtccc cggccatcac
    22021 gagagaggcc tggacgagga acaagagcac gaacaccgga catgacatcc atctcgctgc
    22081 ttgtctcatg acctttcctt tttccagttc acccatgagg cgcctcatcg agccacctca
    22141 cccacggctg ccattttttt atcgggcgct tttttcacca agacccccac cgtccgattc
    22201 ggtttggtca aatattgagc agccacccgc tgcacatccg ctggcgtcac agcggcaatc
    22261 ctggcccgcg cgttcaacac atagcgccag ctgcctgcga ccgtctgata aaacgccaac
    22321 tgcgaggcca gcccgctatt ggagcgcaac gatcgcacca gatccgcatc aagtccgttc
    22381 aggacccgct cgaactcttt ggccgagatc ggctccgtct tcaatcgttc gagttcttca
    22441 taaatcgccg attccacttc cgccgtcgta tggggcgcca acggggttgc cgcaatcacg
    22501 aacagattcg gtgcacgaac gcccgggtag ttcgtatccg acaataccga cgccgcaagc
    22561 cgcttatccc gcaccagcgt gctgtacaga cgagaggtca ctccttcggt cagcacttca
    22621 tcgatcacat cgaacacaaa atcgtccggg tggccgatgg tgggtttgtg gtatccgatc
    22681 gccagtgccg gttccgcgtc gaattcgatt tcgacccggc gctcgccgcg ctgcggcggc
    22741 tcttcggtca ccaaggccgg aatgggaggc gcggccggga tcttgccgaa ggtctgctcg
    22801 atcaacgcaa tgacctcttt cggctggata tcgccgacaa tggcaaccgt cgcattgttc
    22861 gggccgtaat aggttttgaa gaaggcttcc gtcgcggccg gggtgagggc catgatatcc
    22921 gagccccaac cgatcgtcgg cacaccatac tgatgggcct ggaaggccgt ggaggtaaag
    22981 gtctcgtaga gcaggccgtt gggactgtcg tcggtccgga gccggcgctc ctccatgacg
    23041 acaccccgct ccttatagaa ttcacgtaac accggatgcg ccatgcggtc ggattccagc
    23101 gacgcccaca atggcaagcg attggcaggc aggctgatca catagcgggt gatatcctta
    23161 ccggtcgacg cattcagccc gactccacca tgacgttgat agagaagtgc catttcgttg
    23221 cccacgacaa actcgcccgc cttctcctgc aactccttga accggcgctg cagctgttgc
    23281 acctctggcg atgccacatg cggcttgcct tccatctgtg cgcgagcggc ctcgtcccgc
    23341 tcgcgatggt caagctcatt cccgaccacg gtgagttcgt ccaacacggc ctgttcccgc
    23401 tcataatctc gcgtgccgac cgtccgtgtg cccttaaacg ccatgtgttc gtaaaggtgg
    23461 gccaagccgg tcaaccccac ctgttcattc acgccgccga ccccgaacgt catgttgatg
    23521 ctgacgatcg gcgcctgatg ccgttcgacc atcagcacgg tcatgccatt agccagctta
    23581 tgttcgatga ccctgtcggc caggctcggc gactccgcgt atcccggcgc agccgccgca
    23641 agaatgccag agaggatcac aacaccgatt gtgcgcgctc gtgtgatgct gatcatatga
    23701 agagctccat cagttcctcc ggtcgttcaa taaaccaatc cggttggcaa gccgccatct
    23761 tctgacgatt gcccatgcca tatcccaccg cacagacccg aatgcccgca ttgtgcccgc
    23821 cattgatgtc attggtgctg tcgccgatga gcaccgtgcg atccttcttc acgccgagtt
    23881 gttccatcac gtgcaccagc atgcccggct ccggtttcag accaaaccca ttgtcgccgc
    23941 cgaccacgta ggcgaagtga tgatggccca gccctttcaa aatgacattg gtatattcga
    24001 tggctttatt ggtcgccacg gctttggact tggcggcgta atgcgcgagg acccggtcga
    24061 taccaggata aaacacggtg cgatccaggc aatgttcaag atagtgcccg cggaagacgc
    24121 ggagtgcttg atcgaaccga tcctggtttc cttccccgac agccagccgc agcagcttct
    24181 tcacgccgtc gccgacgaat ccgaaaattt cctcctgcgg ccgttccggc agatccaggt
    24241 cgcgcaacgt caaattgacg ctatcggcga tatcccattt cgattcgatg agggtgccgt
    24301 ccaggtcgaa gatgagcaag tcgatctcag actttttcgt cattcttttg cctttcgcaa
    24361 ttctgcctgc aaggccgcca gcgaggcctg cctcgttccg tctttttctt ccagcatcgc
    24421 ctgtcgcacg acattcagca tgcgtttctg accgacgtga ggcgggagaa tcggctccat
    24481 gattttccac cgcccattca tgaccttcag ccgcactcgc gcctgctccg tgcccggctc
    24541 cggcacgaac ccggcggtat ccaggtacat gatacccagg acgcggaatt ccaccggcac
    24601 caccacttcc agccggttca cgatttccca ttggcggaaa tcatccggca ccgtgaagcc
    24661 attgatgacg accactttcc cccaggccgg ctcttccttc cagtcgacat agccggccac
    24721 cgattcgaag gaactggcgt cgaggcgcac ccccttgttg tcgagggcaa aatatttctc
    24781 caccactttg gccggtgaat gcgacaccgc attgctttgc gcgtgccctg gcgaggccca
    24841 ggccgtcagc ccgacgattc ccgccatgat tccgatgatc gccccgcgca gagatatcat
    24901 accgctaacc cacctcatgc agtttcatga gattcgtccc gcccggctga ccgattctgg
    24961 tgcctgaaag aatgacaatg cgttgccctg ccgtagccag accatcggcc ttcaggcgtc
    25021 gctccgcctc gttgactcgc tcatcggtgg tgggaatctg cggcatggtg tgcggcatga
    25081 ccccccaata gagagccatc tgccggcgga ccggctcaaa cggagtgaag ccgatgatcg
    25141 gcgcagccgg acgttgcttc gaaaccagcc gggctgtcgt gccacgctcg ctgaacgcca
    25201 cgatggccgt ggcaccggtc gcagcggccg cggacgatgc ggacaaacag atcgcctcct
    25261 caaacgtcag gtgccggtta tccggcgatg cccgccgtac gaatgccgga cccgtttcga
    25321 cctccgccgc ccgtacgatc cggtccatca cctgcaccac ctccactgga tagtgcccga
    25381 cggctgtttc cgcagataac atcaccgcat cggtgccatc gaacaccgca ttggccacat
    25441 ccgacgcttc cgctctggtg ggccgcgtat gctgcgtcat cgactccaac atttgcgtgg
    25501 cggtgatcac cagccgtcga tggcgattcg cggtggcaat aatgcgtttc tgcaacaccg
    25561 gcaccgcttc cggccccaac tcgacaccca aatcgccgcg cgcgaccatc accccgtccg
    25621 cctgctggag gatagcgtcc agatccgtca cggcctcatg ccgctcaatt ttcgcaatca
    25681 ccggcacatc cccgccgcac tcagcaatca accgtttggc agcacggatg tcgtcagcgc
    25741 cgcgcacaaa tgacagggcg atataatcca cgccctgtgc cacgccgaat cgtaagtcct
    25801 cacggtcttt gtccgtcaac gtcggcgcac tgacgatcgt gcctgggagg ttcatgccct
    25861 tgtgggacgt gacacgcccg cccgccacca ccgtgcattc cactgcgccg tcgataatgc
    25921 gatcagccgt caattctacg aggccgtcgt ctatcagtat tttggccccc ggccgaatgt
    25981 ccctagtcaa ggcttgatag gtcacgggaa tgtcttgcgc gctgtcggcg ggcaagacgt
    26041 tcctcgcgcc gatctgtccg ccggatcgca aggtcatcgt cctcaaccgc acccgttggc
    26101 cggctcgcag ttccaatgtt ccgtcgatct ctccgacgcg aatgcgcggt ccctgcaagt
    26161 cctgaatgat cgccaccgct acgccctggc ggtcagcggc ctcgcggatg accttgatgg
    26221 cctgaccgtg agaggcatgc gtgccatggg aaaaattcag ccgcgccgcg ttcatgccgc
    26281 tctggatgag ttgctcaagc acgcccacgc catcgctcgc agggccgatg gtgcagacga
    26341 ttttcgcttt tcgcatgctg ttcttctgtc ttgacaagat cggcccggca caccatcttg
    26401 tacgtatgcg gtgaggcgcg aaacgttcct gattcggtcg gcaacgggtc attctaacaa
    26461 acccgtccgt atgccgcaaa agaacatctg catggcgtcc ggaggactct cgaggccgga
    26521 ctcatgaaat atttcttccg aagccacaca ccacgaggcg atcatgagcg catcggacaa
    26581 gcaggcaacg gagcaattgg attttgtcac gttggaaggg ttgctgacct acggaacagc
    26641 cctcgcgcaa cgttcatcga gcggccggta cgcattaccg cccccacccc cgccgtcagc
    26701 cgatcgactg cagccggcgc aggcggcggt cgaggggatg agagcgttca tccagcatgt
    26761 tcaaaagggt ctgcctagcg ccgaagccta tcgcgccgcg cgacaaaccc tcctcgccaa
    26821 gacgtgcgga aatgaccccg tggtattttt cgccgcctgg aatctactgc ttgcacgcgg
    26881 ggaacttgcg ccgctgtacc gagcaccgat cggcgcgacc cagaaaccgg cgcaccgccg
    26941 accggtggcc atcgtgccgc ggacgcaact gactccgcag ctggccgaag gtcgtatcgt
    27001 gctggatctc ggtgatgatc gtttctggtt gcttccacgc gatctcggcg accgcacatt
    27061 gtttctcacc atgcgtcacg gtgtttctga ggtcgaaagc aaaactcatc gtgtgggccg
    27121 gcgactggcc aacgtcctcg ataccgaacg cggcgtgccc cgagcggatg ccgtgggcgc
    27181 ggcactggcc cgcatggtgg gcgtggtcgg ccagcaatta ggatatctgc acttgcggaa
    27241 ttacctcgat ccgcgcacgt ttcttcacct catcagtcac agcccgaata cggagcaact
    27301 ctgccgccgc atcaccaagg cgttgctccc cgacggggca gatgccgtac atccccacat
    27361 tgaaccgacc ctggaatcgc aagatttcgg gtgggtcacc ggcatggaga aacaagcgga
    27421 gctcgacgcg gcggcacagg cattcggggt ggacgcgaag accgccaaac ggctcatcaa
    27481 acatccgttt tatagctacc ccggcgggca ctcatttttt gatctctacg tggatgtgat
    27541 cgatgggctg caccggctgg gccatgccca tcccggagaa gtgctctgcc tctatacaca
    27601 cagctcgacc ctgcgcgcgc tgcgcattta cctcgatccg cgtcccttcc gcgaagcctt
    27661 ttcggaattc ggcgaataca aagaaggcca ggacaacgtg gtgctcctca cgttggaaaa
    27721 cggcaaactg tccggctatt cgaccgccgt tgggctgatt gaaagcgacc gggttgcgcg
    27781 tgaggcctgg gtgacggtcg agcgggaacg cactcagcgc gtgacgctta aacccgggcg
    27841 catcaaacga ctgatcgccc tggtgtcagg cggggatttc ggcggcgggg gggcggcctt
    27901 gaaggaattg cgcgtcaccg gcaaccgctt cggcctggac gtcttttttg tccggcatgg
    27961 atttctcggc ctggctaaca attggatcga tgccgtcacc gaagaagata cacgcggcat
    28021 gagcagccat gccagcagcc ccatcggcag cagccggttc gaggatttca aagacgaaca
    28081 ggtgcaacgc gcagcgatgc ggtccctcgc tccgtacctt gaagactcgg cgctcgtggt
    28141 cttaggaggg gacggcagcc tgaggggcgc gcgggccatc catgagacct atggcgtgca
    28201 ggtggtcggc atccccggca ccatcgacaa caacatcgcc ggcaccacct cgctgggatt
    28261 ccattcagcc atcgccctcg cgaatcaatc cattgaatcg ctgaaagcga ccagcgccgc
    28321 catgggcagt atcttcttcg tcgaggtcat gggtgccggg tccggtcatc tggccttggc
    28381 ttgcgcctac caagccaggg cggaaggaat cctggtcaat gagcatcccg atcccgacac
    28441 ctatatcgaa gacattgtcc ttggcacatt acaacgcacc ttgggggtgc cgaacaaaag
    28501 ccacatcttt gtcgtggcgg aacggacccc tcaccggcat caccgggaag ggggtgtcca
    28561 cgggctggtc gattatgtcg cacagactat tgccacatgg cctcagcgtc gtgacgcaac
    28621 gggcccatac tcgctggcct cggctacgaa ggccacaata ctcggccata ccctgagagg
    28681 cgcaccaccc actccggaag ataaaatgat tgcgcaacat ctcgcctacg aaacggttcg
    28741 ccggctcgtt gaacagccgg acaccatcgt cggctgtatg gtggcctatc acgattcaaa
    28801 ccggatcgaa acgattccac tgcatgccgt gtcgcccaag ccgttcgact gggaattgtt
    28861 tacccggatg cacgtcacgg agctgcagca cgaccgtgtg tgatgggttg ctcttccgtc
    28921 gtgcggtggc cacgttgacc gctcctatgt gacgtgttag ggtctttcca gttgctgtca
    28981 cgagttgtga atcaccatgt ctgtctcgcg tgtcacacga cttgtcctct gggtttgcgc
    29041 gacgttgctc ttgcacctgg gcgtcgcctt cacggctgac cctggccctg cacttctcca
    29101 cactccggtt ccgcatctca ctccggcacc ccctcagacg ccacctgcgg acgacggcgc
    29161 cgtgcgcgct ggccccggca gcaagcaagg tgaggtacgg gataccattc cttcggctgc
    29221 acgcgacacc ctcaaagcga ttgaagcgcg acacggggaa cccttaccgg gttacgttgg
    29281 agggcgcaat tttctgaatc gagagcgcgt gcttccacgc ggccgttatc gcgaatatga
    29341 tgtgcatccg aaggtgcccg ggaaaaaccg cggagccgaa cggatcgtca ttaatcaatt
    29401 cagcgggaaa gcctattaca ctgccgatca ctatcgttca tttgtgccga tgaattgagg
    29461 acacatgtcg attactgcgc ttcaatcgat caagaaacct tgggcccatc tcctggtgca
    29521 tgccgaggga gaggcgctgg ataaattgct gtcggtcccc gcccagtttg tcaccaaaac
    29581 cgtctccggg aaaaaatgta aaaccaaggc cgggctcttg gacgaattcg cgcgggtctt
    29641 ctcgtttccc gactacttcg gccacaactg ggatgccttg gaagagtgtt tggcagacct
    29701 ggactggctg cctggcaaag gctacctcgt cgtggtcacg gatgcagacc aggtgctgac
    29761 gaagcccgac gaggaagacg actttgaaac cttcgtcgag attctgagcg aagccggtga
    29821 ggcctggagc ctcaaagaat ccgacgacgc gactggcaac ggcctaccgt tccatgcggt
    29881 gctggcagtc caggagcggc acaaacggac ccgccataat tggttcgcac cgcctctagc
    29941 catggagcgg aaggcgacga aatccggcgg atccaaggga tcaaaaacca gcggtcgtta
    30001 gaccgcacct tcccgtgatg taaggaggca ctgatgggac tcttcgatca acttggacag
    30061 gcggcgacgg ggatgctggg acagaccggc aataacaatc cattaatcca ggccgtggtc
    30121 ggcctgctcg cgcagaacag ccatgtaggg gggcttggcg gacttattca agcctttcag
    30181 aaaaacggac tgggagacat cgtgaattcc tgggtcggca cgggcaagaa tctgccgatc
    30241 tcaccggaac agatccacca gggattgggt ggcgacctcc tcaaacaatt ggcgtcccaa
    30301 gcaggtctca gtacagaggc cgccggcggg caattggcaa atctgctacc cggtttgatc
    30361 gataagctga cacctggagg aaagattccg gactcgacgt tgattgaaca ggggttaacg
    30421 ttgttgcggg gaaaactcgg gtagattcga tggccggctg attctggcta accggcgtgc
    30481 agatgcctag gagagaaaga gcaaccgtcc catctgtcgc tggatccacc gtgtcagtcc
    30541 ccaggacaga tcgttcaggc gcgagagcag ccagacttgc ggcgtgacga cttgcttcac
    30601 gctccggctc agcgaggtga aatgatcctc gcacagccca tgcgtctcac tgagatcgtc
    30661 gagcggcaac ttttcgcgga gaaaggccgg ctttccatct cgcacacacc aggcgcagag
    30721 cactctcact gacgtcactc cattcgttgc agtccgactc ctccctgcag gcgcaaaacc
    30781 cgcaccagga ggcatgggct atcggttcat caatttttca gagggttaga agaaagagat
    30841 tggcggaagg ggaagattcc tgcagacaat tgtgtggcaa tatggcgaca aatgacattt
    30901 tttgtctctt ccgaaaggtg aggtctcaca ctcatcattg caataaggca gacgctactt
    30961 cagatcgtcg gcggatcacc gcaggctcat accgttggcg cgatccctct tcgtagcaag
    31021 gaaccagggg atcccgcccg attcccatct gctgaagatg gctggcacaa atgccgtgag
    31081 tttcgcgtcc atcggccaaa ggagcttttt cccgcaccac agccggtcgc ccttcacttt
    31141 cacaccagga gcaaatcact ttcatcatgt tctcctacgc aatgacaccc ggtgttcagc
    31201 aaaactcgcg ccaccaatcg acaccaggaa aaaccatgat ttttaaaaga aactctgatt
    31261 gaattcatga agcctcccgg ctggtgtatc gattcagata ccaagctgta tcggatcatc
    31321 tgccggggcg cagggaaaag cggtgcatca attgaccgtt ttgaatttcc catgttaggt
    31381 tgcgccccaa ttcaaccctc gctgaaagag gcccatgaaa acacaacgtt cggaacagct
    31441 ttttgcagag gcacagcaga tcatccccgg aggcgttaat agtccggttc gagcctttcg
    31501 ctcagtaggc ggacaacctc gcttcatcgc ccgcgccaaa ggcgcgcgcc tgtatgacgt
    31561 cgacggcaac agctatctcg actatgtgct ctcctgggga cccatgattc tcggccatgc
    31621 gccggccacc gtgacaaaag ccattcaaca ggccgccgcc aacggcacca gttatggcgc
    31681 gccgacggaa ttggaaattc aattggccac gatgattcga gaggccctgc cctcaatgga
    31741 gcaggtgcgc ctcgtgagtt cggggaccga ggcggtgatg agcgccctcc gcgttgctcg
    31801 cgcctatacc aaacgcgacg gcattctgaa attcgagggc tgttaccacg gccacagtga
    31861 ctacctctta gccaaagccg gttctggtct gaccacattg ggtatccccg actgtcctgg
    31921 ggtcccggaa gattttacaa aacacacgtt gaccgccccg tataacgact tggtgacggc
    31981 ggagaagctg atcaaaaagc atcaccggca gctggcctgc gtcatcgtcg agccgatcgc
    32041 cggcaacatg ggggtggtcc ctccttcgcc agaattcctg gaaggcctgc gcaagctgac
    32101 cgatgcccac gggatgctgc tgattttcga cgaagtgatc tcgggattcc gggttcaata
    32161 cggcggcgcg caaacgctct acggtatcaa gcccgatctg acgattctag ggaaaatcat
    32221 cggtggagga ctgcccgtgg gagcctacgg gggtgcgaag gacatcatga agatgattgc
    32281 cccgtccggt ccggtctatc aggctgggac gttgtcagga aatcccttgg ccgtcacggc
    32341 aggcatcgag accctgaagc ggttgaagaa accgggtacc tacgaacaac tcgaacaacg
    32401 gtcggtcgcg ctgtcggaag gattgggaaa aatcgcgagc aaagcgggag ttccgctgac
    32461 tcagacgcgg gtcgcctcca tgatgtgcgc gttctttact cagggaccag tggtggactg
    32521 ggcgactgcc aagaaatccg acacgaaggt ctatgcgaaa ttctttcacc acatgctgga
    32581 tgcaggggtc tacctggcgc catcacagtt tgaagccgcc ttcatgtcgg tagcccatac
    32641 tccgcgggac attgaacgga cactcaacgc cgctcaagcc gcgtttaaaa ccctctgacc
    32701 tggtgtgaag atggcgggag ttgctctttg tgaagccggt tcccggatgc atttcgtatc
    32761 gaaactgata cgaaatgcat ccattcggat acgcagctga agaccgattt cagcattctt
    32821 gtctattcgt cgtcgtcctc gtcgtccata tctaacgctt cctgccgttt ctgctcttcc
    32881 atcgcattgt gcaggagcat gcggacaaac attgaataca gcgaaccttt ttccaaggtt
    32941 ccgccgggag gaatcgcgat cttgccttcc ttgatcatgc gatccatcca ggccttttga
    33001 tcgggcgcga tcaaaatcgg cacctcgacc agccccactt ttccaaacat atccatgatg
    33061 taaacctccg tgaattctca ctctttgttc gtagctcgcg gaaacccggc gagctgaccc
    33121 gctcttccgg caacactgcg cttcccgcgt cacgacggac gtgcgtcggc atttgagcac
    33181 gcggtttttc ggcttgtcaa ccgaagagcc cagccgtgac tcatggcatg gagcgtgctc
    33241 taacgagagc tgaaccaacg gaggacgttc agtatgcgct gcaccatcgt acccgactcc
    33301 cgctttttga gccggttcgc cctgctgttg ctggtggcct ctcagaccac gctcgcacac
    33361 ggcgcagtgg aagaaagcct gtcaacattg cacacctaca cccctgcgat ccggaatgat
    33421 ccgagcccgt ttcactgcac cgattgctgg gatctgaagc ccgatcaacg cccctcgctt
    33481 cctcaacacc aatcccaacg gtatcgacgc ggacatcatg agcgaggtct tcgcccgcat
    33541 aagaacccct tcgcgaaaaa atcgcattcc ttcagtcgcg gaatgagatc ccttccgcgc
    33601 catgccctgt cgatgggagg gttcgagcga accgtggaag gctggcaagt ccgcacagtc
    33661 gatggcgata ccattcggta cggcactgac cggattcgca ttcgaggcta caacgcgccg
    33721 gaattgtccg aaccgggcgg aagagaagcg gcccttcggc tggagcagct attgcacgat
    33781 gggatcatca acatcatccc ccacggccac gacgtctacg gccgcaccct cgccgatgtc
    33841 tttgtgaatg gacacaacgt ggcggacatg atgacattgg aggggtacgg gcggagggga
    33901 taggacagcc gaatgcattc gtccaggctc cagtctccgt taccactctc atcaccatga
    33961 gacgatggtg atgcactccg cctcctcacg gtcggattag caacttgatt ctcgccacga
    34021 tcggcgcaat agtgctgagg ctctctgtgc gtcagcaaga tgtcttcaaa ctactgcgac
    34081 aaggaactca tgcctaaccg attgcactcg atcctgcttc tcaccggctt attcgcgctg
    34141 acagcatcag catggggggc cgttccggcc ggaaacacat tggacatcgt gatcggcaat
    34201 ggaccacatg ccggaacgta caagccgccg gcggagacag tgatctgtct gcacgccaaa
    34261 acgcagaacc gctattcggc ggcgtggaag gatttcgacg cgcacgatgc gaaggccatt
    34321 gctgaagccg gaatttcggt atcgaatccg gacggcgcgg gggcaaaaca gggcgacgta
    34381 cgcgtcgcgt tcggagaccc ggacaagaaa ccgaccgtct acagcatcga tcaggcaccc
    34441 ctcaccatga cgaagaatgg caaaggcgcc gagatcacgt tccaaggaaa aaccaaagat
    34501 ggcatccagc ttcgcgtgac ggcgaagtgc tcggacgttg aggagatgta acgcgggcac
    34561 gtaccagagg cggcaccgat agcccctgcg tttccacgct atgaagactc agtactacac
    34621 agccaccagc ctcgacgggt tcattgccac cgaagacgat tcactagatt ggctctttcc
    34681 actaggcaac gtaaacgaga caagttaccc ctccttcatt gctgaggtcg gggccttagc
    34741 catgggggca gcgacctatg agtggatgct ccgccatact gacaagattg ccgaccaaca
    34801 gactggcgcc ccctggccct attctcagcc tgcatgggta ttctccaccc gtccgcttcc
    34861 catgatcccg aatgccgata ttcgcctggt tcgaggagat gttcgaaccg ttcacactga
    34921 catgcggcag gcagctggat cgaagaacat ttggatcgtc ggaggtggag atctcgcagg
    34981 acaattttac gacgccggtc tgctggacga aatcattgtt caagtcggtt ccgtcacgct
    35041 gggacgcggc aaaccacttt ttccacgccg cgtcacgaat ccgtcacttc ggttggtatc
    35101 cgtgcacagg atcggccccg gcttcgccga gttgcgctat gaggtgccga aagagaagca
    35161 ggccgatcag gcctagtcgt cccaccggca gagcggcgtc attcggtcag gccgcaattg
    35221 cctcctatcg attgtagtca tcacgctcgt ggcttttacg gggagacaga catgaacgcg
    35281 aaggacattg cggaattggc gaaggcgacc atgaatggat cgatgccttt tcctgagatc
    35341 gtcggcaaac tgatcgccaa cggtgtcgag tactatcatg tggactatgc cctcagttcc
    35401 ttttccttct acaccggctc ggggacagtg gtgaccgccc cccttgtctt cgaaggcctg
    35461 ccttcgattt cagagcactt cgatacagcc gcgctcaaag cggccattct cgacagtcag
    35521 cagcatggac aaaaatttag ggcattttgt gagcgcgccg tcaaggcggg ggtgcaaggc
    35581 tactttgcgt tccttcgggg taagcgcgtg acctatttcg gccgccaagg ggatcagcat
    35641 accgaatggt ttaccggcgc tcggcccaac gaagcgtcgc agtgatcaag aggccacgtc
    35701 tcttacgggc tcctcagcga ccctatgaaa cccctgctag ccagcctttt tctcgtcgca
    35761 ctcctcccag gagttactga cacgaaggcg accgagaaac gacccctcgc ttcccatcgc
    35821 agcggggttg agccatcaaa gaccggtgag tgctccaccg accatccgat caaaggcaac
    35881 ttcaccaccc gtagcggcga acgctgcatc tatcacatgc ccggacagcg ctggtatcaa
    35941 cgaaccaaag ccgagcgctg ttatgcgacc gaagccgaag cagtgaaaga cggttgcagg
    36001 cggtcgaagg tgtgagacag atcatgaggg aggactcgta gttgaagtct gtccgaactc
    36061 aagtgagagc catcagcatg gtactcgtga cgtctagctg tctgcctccg ggattgacga
    36121 ccacccccgc gccacgtaca agctagtgca acgatcagcc aatccacatg acatcttcaa
    36181 tgcgcctctt cgtacttaac agcatgatgc tttcggcact gcttacggcc tgcgtctcga
    36241 ccccttcgcc ggacgcaatg aaacacatcg ctgaacgcaa tcgttgggaa cttgcccact
    36301 ggggcaacca ggccattccg tatgaagacg atggagaggc ggtgatcctc gccttcaagg
    36361 atgggaaagt gtcgggtcag gctggatgta atcggtatgc ggcggcaatc acgttcggac
    36421 ccaagaccgg cgaggtctcg gtatcgcacg gcatcaccac gcgcatggcc tgccaaccca
    36481 agcgcatgga atttgaggcg gcgttcatcc gtgcctttga agcctccacc cggtatcgac
    36541 tcgatgggga aagcctgttg tttgagagcg ccattgctca gccgctggag ttctatcgac
    36601 gccccccgga ataacccatc tctctctgat ttcacatcga gtctgtcctt cgcgtatccc
    36661 ttccgatacg acatcgtatc gcttgtgata cgacactcag gagaagcaca tcgattctga
    36721 ccgctggcat tggtccctgc tctcaaaata ggccgctgcg tttacatcca ttccataaac
    36781 ccaccgggca ccgctcttgc tcgatacatg ggtgaacttg gaatccctat catggctggc
    36841 aagatcttca gcgcgctcct cgcttccgga ttcccgaatt ccatacggct gtgcggcaag
    36901 gtcatggttt ctgaggagaa tgcagatgcc cgttccatgt ctcagccacc ggaagggaga
    36961 gcctcgggga gaccggccaa aggaaatgtg gttgctctgg accgcctctt tccatttcta
    37021 tatggagggg tccttgcggc aagcctgtgg tggatctgga tgacgctggg gcgaccgttc
    37081 catccgggct ggattgccgc accgctcctg gtgaccacga tagcggattg gttggagggc
    37141 ctcatgctcc tggcgcaact tcgccactac atgtcgccga atcagcaata tgccgagagc
    37201 ctgttgatcc ggctttccgg ttgcgccacg atcatcaagc tatggctcac cagcggcctc
    37261 tacgtaaccc tggccggact tgttctgaac ttgctattca cattcccggg ccggcgcctg
    37321 gcgagtgatg ccgctgaata atccggcgtt cccgcccggg acctgtctat ggaggcttct
    37381 atgaccagcc ttgaagcgtt taccagccat cccaccgtag aagcggccgt ccgggacgct
    37441 cagacggcca ctgaaggaaa gcacctgtcc gatattgtcg atgatcacgg gttccaatat
    37501 gtggacttcg tcatggaagg cggaggggta ctcggcattg cgctgaccgg atacacctat
    37561 gtgttggagc aagccggcat tcgctttctc ggcatcggtg gaacctcagc gggatcgatt
    37621 aatgcgctga tgattgcggc cttaggcccg ccggaggaag ccaagagtga acggctgtta
    37681 cacattctgg ccgggatgcc gatggcgagc ttcatcgacg gcgatcagga tgcgcgagac
    37741 ttttctcagt ccgctttggg aaaggtcggg atgatgaaac tgcttttgaa ggccatgcag
    37801 gtcgtcgaca acctttctga tgacttggga ctgaatcctg gcacggcctt ctatgactgg
    37861 ctgaccgccg aactcctgaa agtcggagtg agaaccaatg cggatttggc caagcggcta
    37921 caaacggtgc cggcagggct gcatcttcgt ccagaccgga cagacaggca gctgaccgaa
    37981 gcagacacga ttggccgact catcatggtg gccgccgata ttacgaccga gacaaaggtc
    38041 gagtttccca ggatggcgga actgtattgg gccaaacctg acgaggtcaa tcccgcctat
    38101 ttcgcgcgcg cctccatgtc gattccgctc ttctttcacc cattccgcgt caacaactgc
    38161 tcccaggaca gggcgaagtg gaaagacgtg gcgtcatatg atggcgtccc tcctgacacg
    38221 gtcatgttta tggacggcgg catcatgtcc aactttccca tcaatctgtt tcatcagccc
    38281 tatcatgtgc cggcttctcc cacattcggc gcgaaaatcg gcaccgaccg cgcgcagccg
    38341 gccgtcatcg acaaaccctc acaactactt ggcgccgtgt tcgatgccgc tcgtcacacc
    38401 ctggattttg atttcatctc gcgaaacccg gactacaagc acctggtggc catgatcgat
    38461 accggcgatc acaactggtt aaacttcagc atggaggaca cagacaagat cgacctcttc
    38521 gctcgcggcg cggcagccgc cgccgaattt ttgcagacat tcaattggac gaaatataaa
    38581 tccattcgca aaggtattgc cgacgccttt cttgcgagca atacgaacaa tggaggatct
    38641 ctgaaacttg ctggcaatag ccagaggctc gtcgcactca gctctcacta ggtcgtgctg
    38701 agccagggac aataccggca gtctggagga ggcggagtca acatcgcctc cttcacgcca
    38761 tccctgtacc gcaatgcgcg actaggcaca gttgatctcc tcgtgatcct gcgatacaca
    38821 tggggcagga tcttgtgggg acctctatgc accgcaatac actatccgtc tctttcctgc
    38881 ccgccctgct gtttggagct ctgcaattat ttcttgagcc gactgctgga tcagcagaac
    38941 agatccgcgc tgcgatgtct gcccctcagc ccgaagcctg gtggatggaa cggcatgaac
    39001 gcaccgtcgc ccggatacgt cagggccatg tcgatttgct gtttatcggc gattccatca
    39061 cccagggatg ggaggaggac gggcgtcccg tctggaacgc cttctaccag catcggcatg
    39121 cggcgaatct cggctacaac ggtgaccaga cagacaatgt gttgtggcgg cttcagaacg
    39181 gggaactgga tggcatcact ccaaagctgg cggtcgtgat gatcgggact aacaacgcaa
    39241 ccaggcgcga ggatcctccg gaagagacgg ctgccggaat ccaggccatt cttacgacct
    39301 tgcgcacccg tttgccggac acaaaaatct tgctactggc cgtctttccg cgaggccttt
    39361 cggccgatga tcggcttcgc cgcgtgaacc aggcagtcaa cgagcggctc ccggctttcg
    39421 ccgaccagcg ccaggtgttc tttctgaacc tgaaccggcg ctttctcgac gacaacggac
    39481 gtttacctga agagatgatg cccgacgcgc tgcaccctgg tgaacgcggc tatcgcgtat
    39541 gggcggaggg aatggaagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
    39661 cgcgctgcac cctggtgaac gcggctatcg cgtatgggcg gagggaatgg aagacacgat
    39721 taagaaattg atgggcgagt gacgaatgcg aagccggacc cgcgccgtcg caggatccga
    39781 actaaggttt tttctttttc ttgtcggaag gattatcaga gaagagcagc catgcgagga
    39841 cgacgaaacc gatcgccacg cccgtcaccg ccagccatgt gccgagttta tccattagtg
    39901 cctcgtgtgt ggaggtcctt gtaacgcagc aagggtgagt ttgtcgagcc gcttcggttt
    39961 caactgctcg tctaccgtca ccagcttggc cgatccttcc accacaatac gcccgctcgt
    40021 ccgctcccgc aacacatgcg caaaggtgaa cgacgcctga ctcgcatccg tcaacgtggt
    40081 gtcgatcact aaggtgtcgc cgtaccgtcc aggtgaccga taatccagtt cggcatgcac
    40141 cacaacgaac tgtgtgccct gatcacgcag ccctgcgact gacaatcctc gctcttcgag
    40201 atagtgcgtt ctcgcccgtt caaagtactt gagataattc gcgtagtaca ccaccccgcc
    40261 gcagtcggtg tcttcgtagt aaatgcggat gtccatcgtc ttccgtatct cgtgaagcgt
    40321 atctcgtcaa agccgtttga taagacttct gaccatcttc gactcgtggt caagttgctc
    40381 caacagctcg tctcgctgaa caaccgtgat gaatgtgagg tcctgagcga tttccacgtg
    40441 ggtttccaat tcagcgcaag acccaagcgc gacatagaga aactgtcggt attccgcatt
    40501 gtgtttccga ttgaatccct ctgctattat tcgagggaat cgatatcgct gcgcgacgca
    40561 tttgcgacac taatccgtag atctcttcct tggggaacgc cttggtcact cgatagacat
    40621 cgagcacgat attcttgccc agcttccata tttcaagatc gcgaaaattt cgtatctttt
    40681 ccatccggct gtctggtcca acgagatacg cttcacgctt cacgaacgac ggttaactag
    40741 agactgagga cggggaactt gggcaacgag gattcactca cgccggtaag cttcatgacc
    40801 atatcggaga gctgctgcac ccgctcccct ttgaccgcct cgaacccatg gcccaacacc
    40861 gcatgattgg cggcatctag cagtggtttc atcttcggcc agtcgcggag aaacgcctgc
    40921 cccatctgat cgccgaggcc ggccaacgtc cgccactggc tttgcaaagg cagcttatac
    40981 ttgccgtcga catcgtcgag ataagaggtg cggcaggtgt cccgtaaggc ctcaggcaat
    41041 tgatcaggct gcacatccca cgttttgatc ttgtactgtt tgaacaactg ccgctgcgcg
    41101 caggcctcca acgcgcggac gagcgccacc atggcccgct caggatcatg gtccccatgc
    41161 gcgcggcgat gcgcgtgcgc cagcagatcg agcacgacac cttctttgac ctcggcgggg
    41221 tcgagcacca tcttttccaa aaagccgcta ttggccttga tcaacggcag caacgctttc
    41281 agacccggcg gcccacccca gatcgaggcc atatcgagag ctttcaatga cgtcttcagc
    41341 ttttcccagg cctgacgata ctggaactgc tcccagagtc catatcccag tgcaagttca
    41401 gccagggcgc gatacaacgg tttctgtccg cc
//
LOCUS       DAOQDS010000038        40447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_312_length_40447_cov_22.265746, whole genome shotgun sequence.
ACCESSION   DAOQDS010000038 DAOQDS010000000
VERSION     DAOQDS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 40447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40447
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_312_length_40447_cov_22.265746"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1859
                     /locus_tag="PK589_10955"
     CDS             <1..1859
                     /locus_tag="PK589_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ20164.1"
                     /translation="ILSSDMGLILNLTNNLGEQVVRLTMSKLRMNVKASPIIGAALGA
                     LLLSASAVGIGQAADLPEGFAKGDLAPAPPADMIEAGKRVYFTKCVWCHGVDGAGDGP
                     GADRLWPRPRNFNQGTFKIRHTASGELPLFNYNEKTPDSGKNDLFDTVTHGLPGSAMP
                     PWEGILTEEQRIQVLSFVTTQLVKDRKFTDKQSETQTILQLGTIKPAEATEDSIKKGA
                     QLIVDKKCIECHGVEGRGDGNAFNLKDDWGFSIQPANWHKCWNFRGSRQDPYNVRNIF
                     RTFSTGVNGTPMPSFADSTSIEDRWNIANFVNSLCERDEEGKPLGIDPLTDKPKINFV
                     IPSSPVEGEISSDPENEMWKKQGRRYVAMGGQITHKPRNFVNRIDDIWVKSLYNDKSV
                     VYLIQWDDRTKSIAEGKLPWAPTQVNVENFGVKEQAPKTGEEGSIAAAQNNYAVYNDG
                     IAFQFPIKWQEIPAPFKPRYLFGDAKFNADILKWEADGSLRSFKGTGWDQDFEERDDF
                     EEKVKLLKSEWKNGQWTVMISRPLKGSKDDYDEYTRFDIGKYIPMVFFAWDGHNGDAG
                     RKMAVSAFYYTILQPPIPQEVYIYPAVIAVGVVFLEGWMLTRRANKKKGKVL"
     gene            1960..2568
                     /locus_tag="PK589_10960"
     CDS             1960..2568
                     /locus_tag="PK589_10960"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNJ20165.1"
                     /translation="MIDDISGVLVAGGRSRRMGQDKRFMKVGGASVFERTLSVLRVTF
                     AENIVVLAEPIEGLDVQRCPVVYDLIPNAGSLGGLYTGLMTASRSRVFALACDMPFLD
                     PEVIRFMLSQDESADVIVANLGGRFQPMHAVYSKRCVPFLQAMAQRNELKIQNLFHQN
                     ALRMKILTENHLQAFGPGFRSFQNINTPSELASAEASPSIHP"
     gene            2637..3584
                     /locus_tag="PK589_10965"
     CDS             2637..3584
                     /locus_tag="PK589_10965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNJ20166.1"
                     /translation="MLLARPVLRALRCQFPQHEIALIAANAVGSLLCHSAELDRAFSL
                     EENYLSQLFAGFERLSDSFRTWLGQCELVVGWLSDADGAVTNTLRLAGVQQIQLQTPF
                     SSALATTHQADRYWEAVGMEGQREDFTSSLMLPAELERQGQDVLQAYDWDGSSPLILV
                     HPGSGSHNKCMDAWRLAKVIEWLIEARMAPMLLEGPADCRAVAQVLSSLTVPVPVIRE
                     LSLSVAAGVMAQAKLYLGHDSGMTHLAAALAVPTIACFGPTHASRWSPQGRAVAVISG
                     MPCVCSDWSKVETCHDKVCLHIPVESLIEACRSQLLRLS"
     gene            3683..5437
                     /gene="argS"
                     /locus_tag="PK589_10970"
     CDS             3683..5437
                     /gene="argS"
                     /locus_tag="PK589_10970"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNJ20167.1"
                     /translation="MAQGVVQDRVADALAGALRSAQQRGILKIEQMPTINLEAPKRPE
                     WGDVSCTVAMSLSASERRPPFEIAQIIADHIQDRDALFSRVDIARPGFLNLTLQPDLW
                     MEVLRHIETQRAEYGHSRLGQGRRVLVEYVSANPTGPLHVGHGRGAAVGQALVRLLRA
                     TGHEVVSEYYINDAGRQMKLLGVSVLARYLESCGQAVPFPEDGYQGEYIRAVAARVKA
                     EQGVTLLSLSSSEAEQRSKDFAYRELLALIRQDLETFGITFESWFSEASLLSSGAVEQ
                     VLSELRSRDLLFDQDGAQWFRSSAYGDEKDRVVRKQEGDYTYLASDIAYHRDKLRRGF
                     DLLVDVWGADHHGYIPRMQAVVQAYGYPKERLRVVLVQMVNLLRGGRKVEMSKRAGEF
                     ITLREVMDEVGADAAKFFFLMRDSSTHLDFDLELAKQQSQENPVYYVQYAHARISSLL
                     RVAASRGIECPLPSQADLKLLNDPDELAIIRKLSVFPVVLEASAVELEPHRMAYYLRE
                     LAGLVHPFYNKHRILPPMTDLDATPAAPTEPSASTSRVPEAVSPGLTGARLALMWSVQ
                     QVVRNGLTVLGVSSPEQM"
     gene            5450..6607
                     /gene="tgt"
                     /locus_tag="PK589_10975"
     CDS             5450..6607
                     /gene="tgt"
                     /locus_tag="PK589_10975"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNJ20168.1"
                     /translation="MLSFRITHEEAGGRARVGVLTTGRSEVATPAFMPVGSLGNVRSV
                     DGEELLKMGYGLILNNAYHLYLRPGHKVVEELGGVHGFTAWPGAILTDSGGFQVFSLA
                     KFCKVTDEGVVFQSHIDGSSRFISPETSIEIQEALGADIIMAFDHVVALPSSLDQVRA
                     AAKRTSLWARRCVEAKRRTDQSLFGIVQGGLDQSLRVESARDLVSVGFDGYAVGGLSV
                     GEDKADMYAMLDVTVPELPSAKPRYLMGVGMPEDLVEGVARGIDMFDCVVPSRHGRTG
                     WLFTSFGRVVIKQARYARDEQPIDPACRCPVCTRYTRAYLHHLFGVKEMLGARLNTIH
                     NLWFFAKLMEEIRNAVRSGTFQEFRREFHRTYVVDRPGGEGRSDVDNTNMS"
     gene            6628..6948
                     /gene="yajC"
                     /locus_tag="PK589_10980"
     CDS             6628..6948
                     /gene="yajC"
                     /locus_tag="PK589_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNJ20169.1"
                     /translation="MWDSVAWAQGTSGNAGAGGGLLSLVPFVLIFVIFYFMLILPQQK
                     RQKQAKAMLETLKKGDKVVTASGIWGTITNLGKETVTLQIADTTKIKIQKEHIARLRG
                     EEED"
     gene            6984..8633
                     /gene="secD"
                     /locus_tag="PK589_10985"
     CDS             6984..8633
                     /gene="secD"
                     /locus_tag="PK589_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNJ20170.1"
                     /translation="MKKVGGRLLALVTMVVASIIFFLPSYQPLYKELPRWVREVLPDK
                     GITLGLDLQGGIHLVLEVEEDRAVEIAVERTAAGLQDLLVEKKIPAESVKRTGSSQIT
                     IGFQNAELKAQVQKLLDEFPTYVENEGAGSGNKVVWELREAEIKRIKDSAINQALETI
                     RNRIDQFGVAEPLIQRQGLKQVVVQLPGIKDAKLAKDLIKQTALLEFKLLDDDNQLKL
                     DLPGRIQKGKEREEAFLKQVEAKVPEGDQILFERIVEKESGQEWLAPYLVKKRVMLAG
                     DVLSDARVSIGQFNEPYVSVTFDAKGAREFDRITGENVKKRMAIVLDNTIYSAPVIQE
                     RISGGRAQITGTFSMEEANNLSIVLRAGALPAPLKIIQDLTVGPSLGQDSIEKGIKAT
                     LFAGLLVIVFMAIYYRLSGVIANFALMLNLICLIGSLAALNATLTLPGIAGIILTIGM
                     GVDSNVLIFERIREELRQGKAVRVAIDAGYDKALLTIVDSHVTTLITGFALFLFGTGP
                     IKGFAVTLCLGIAINLFTALVGTKVIFDLFNQRKKIEQLSI"
     gene            8646..9563
                     /gene="secF"
                     /locus_tag="PK589_10990"
     CDS             8646..9563
                     /gene="secF"
                     /locus_tag="PK589_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNJ20171.1"
                     /translation="MFEILGKTNIDFMGKRTISFALSGILAFLGIIAVLQIARGAANL
                     GIDFAGGTAVQLKFDQSIRIDEARRALESNGLGSAELQEFTQDNKLLIRVKASTTIEE
                     KVAERVMAVFSKEFPGNKFVVDSSMEIGPTIGKKLQEDAMIAVLISFVGIVLYIAVRF
                     ELRFGVAAALATFHDVLAVLGVFYILDKEITLLVVTALLTLAGYSLTDTVVVFDRIRE
                     NLRTRRREDEETIINQAINQVLSRTIVTSLTVVIVLIPLVLAGAEVLHDFSLALLGGV
                     MFGTYSSIFVASPLLLLWPGSAGSLLKRR"
     gene            9639..12815
                     /locus_tag="PK589_10995"
     CDS             9639..12815
                     /locus_tag="PK589_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ20172.1"
                     /translation="MLFWSRSHSLQRKIITAIVMVGLLPLGLSLVLTYLEERRALRET
                     IGANFKEVAVEAARRIEMQVTRGINEAQQLAATPFLRTSVTESNRTYLDKDEKTVQSM
                     IKAWQQRWRQRDKQSEFPLFINRIVTNYLIRWHDIRKTDYVGIFVTDNRGALVVSSIP
                     QVEYYYGKTAWWQAFMKRGTGKVFVSDIFFDPAFGTHVVNVSAPIIDDEQHTTIGAVT
                     VLLRRDTLFHSVSEATIGQTGHAMLFSSDGAPMICPILSPEEHVVKPELVAAINSSAA
                     GWTTAENDSHGGKNSIVGFAPVRLGADLTPESLGGKRWVTFVRQDPAESYAPLSRLVV
                     QVTVYGLGVFAVLVLTGLTVARRIARPIGLLHEGVQKIGSGRLDQQLDLKTGDEIEQL
                     ADAFNKMAANLRVSFEQIEHRMEDVRRLEARYRDLIEHSPEMIYQLNKAGQFVHVNKT
                     GLDKLGYTLEEMLQMRLWDLVSKGREAEVLAYLERLVAQGRSTIETVFVAADGRPIDV
                     EIHSTALFEIGGGLVHSRAFVRDVTERRLLEEQIHRYTTRLEQAVNERTQQLVASQER
                     YKALFDLVADSVFMVGEDGVIVAVNKREEQALGYSEQQVVGHSILDVILPAHHEDMRS
                     LLAKIHSGERQVPTQEITVRDAGGKETPVEMDVILVRGSDHTWVMVQLRDITDRKRLE
                     RQMHTYQQELEAKVSERTREIEETKQYLENLLENANDVIYTLDSEQCFTYVNSKVLAW
                     GYAKEDLLGRPYLGLLSKRHRGRRLKSTLDIGVKQVYEVEVLTKSGEPRSVMVSVSPL
                     HGVDGEILGVLGIARDMTDTKKLEQQIRNSEKLASVGKLAAGVAHEINNPLGGILNCL
                     YNMRKGTLSPSRQEEYLASMEDGLRRVQRIVRQLLDFSQQHNPELALADINQVVNRVL
                     LLTDHLFVPHRVQLETDLAVDMPEIMIDRHMMEQVLMNLVLNAIQAMRNGGVLTIRTT
                     VKEAHCLVRVQDSGCGISSSVLPRIFDPFFTTKNEGEGTGLGLSVSLGIVERHGGRIV
                     VESEVGKGTTFTVSIPLSTERYAIGRFS"
     gene            12812..14263
                     /locus_tag="PK589_11000"
     CDS             12812..14263
                     /locus_tag="PK589_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ20173.1"
                     /translation="MKGLKIFIVDDEPLMRLSMQDALEGVGCEVMAAATGTEGVAVLG
                     TRQFDIVITDLRLPGADGLTILKTCKERSPTTEVILITAHGSVDTAVGAIKLGAYDYI
                     TKPFQMDELLLIVERVGKIIGLRRENLELKEVLEDRFSFGGIHGRNHQMRALLDKIKL
                     IAAADSPVSLVGERGTGKELVAHTIHLNSPRRDQPLIKMCCADLPDHLLEAELFGHEK
                     GAVPGASRQRRGRFELAHKGTLLLDDVDSLSPLLQSKLWRVLQERKFERVGGRESIEL
                     DVRILCASRQDLKEAVAQGRFRQDLCDHLAAVQVVVPPLRERREDVLVIAEHVLELRS
                     KSLNKSPKGFSQPSCDLLLHYSFPGNVGELEQMVERAVTLGRNGEPLQPWDLCGFQSC
                     PYLGGAPQATCGFCDEGLSAKADEPAAQAATTLATAREAFEKDYIETVLKQVDGSRTS
                     AAAVLGLSRKALWDKCKRYGISSAKGEAEERDE"
     gene            complement(14265..15371)
                     /locus_tag="PK589_11005"
     CDS             complement(14265..15371)
                     /locus_tag="PK589_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNJ20174.1"
                     /translation="MSNSTTTPPTIPSAAPAPTQGASAQAPAIDHLEYWKTGFRELKT
                     FRGHSHGVWTVAYAPDGQTIVSGGVDRYVRIWDIETGRLLRSLRGHTADIRALVFTPD
                     GRTLASGSEDRTIRLWNPKTGEPTKLLFTRYDHNVCSLSLSPDGLMLARGSHNKDIKI
                     WEVTTGTDLMTLLGKDQYDHHWSVCVAFSPDGVHLASGSDIGKIRIWEVLPSGEEKIL
                     HNGHWEETAEDSTETRGFFIEDDGGFQKPMEFWIGAMTFTPDAKILITGSRDNTIRFF
                     EMPTMNELRVLRGHNGWVRSLTVSPDGKVLISSGDDNTIRFWDIATGRNFRTDKTHTG
                     PVRGVALSPDGLRLASASWDRTVKLWEGGQEPAE"
     gene            15568..16599
                     /locus_tag="PK589_11010"
     CDS             15568..16599
                     /locus_tag="PK589_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20175.1"
                     /translation="MTTGVEVSKPSMEIRFQPALLQEVIDSFIEKTEREGDPTYYKEF
                     HELADPIYEKFTLDDREAEFKKLYQYLFGIWGFSDIIRDAFNEFPDLKARVGVVLVKG
                     VLKEDQEGVDVLRKWGSVEHDLAKQFEEKGLKGVGIKLIPRRFYDPALTRYCRHELMH
                     INDMLDPAFGYDPDTRVGQNPGEETLILHRYRILWNISVDSRLIAAGKEPMLQKEDRF
                     KEFRSWYRKIPPAQLKSVFEGLWQTSYFTHSELVEMATDTLRILDRAVDVEGGEIPET
                     QNKVMLMPGFPCPLCRFPTYTWVEDMETKLEPYILDFIRENHPGWDVEYGACDRCVEV
                     YKLRADGVM"
     gene            16612..17217
                     /locus_tag="PK589_11015"
     CDS             16612..17217
                     /locus_tag="PK589_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20176.1"
                     /translation="MAGDPKYGRRDFLKDSVVSVAKAAREFAIHKDAPREQPAAPVRT
                     DWLRPPGAVDESTFLERCTRCSDCIKACPPGAIVSDLSNGTPVIFANQVACELCEDFP
                     CIAACATDALLPVADVVEVRMGIATVSHRVCTAGQGCHACVSKCPVDALSMDFHALRL
                     DVEQARCVGCGMCEQICKTVNDRIAVKVVPARNLSAGSLNY"
     gene            17427..18404
                     /locus_tag="PK589_11020"
     CDS             17427..18404
                     /locus_tag="PK589_11020"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HNJ20177.1"
                     /translation="MTSKQVVQPTSPSSTAIAPPKAVPIKDSPAVERALNRSKIYLLF
                     SWSLLYPEDEEFLDYLQCGEFVEDGRAALDGLRLALDGIGGDRAIQKIALMKKQFDQI
                     EKLVSAECVNWQIGDLQTEHRRVFTNVITLDCPPYETLFGNDHVFAQSHVMGDIAGFY
                     KAFGVELSKDVHERLDHLSVELEFMHFLTYKESYSRCHDGIDKTEIVVDAQKKFVKNH
                     IGRWVPLFCRMLAKKSDTGLFKLIADCMSEWMDFEVAFLGVTVQPYSEADYRPATFNA
                     PEGQTYECGAQDKGNELSMLLSEVGAESFMDQKTKEKDGEKSEGPVGTA"
     gene            18504..19457
                     /locus_tag="PK589_11025"
     CDS             18504..19457
                     /locus_tag="PK589_11025"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNJ20178.1"
                     /translation="MRLVQTRNKRLVFGILFSALIVGIMLTIGQVPLAVSQPVTIPVK
                     AIKGVIPMDGANPIWEGVPGVIIPLSGQTITTPMHPNISVKSVFVKAVSNGKDLGLRL
                     DWSDQTKNDTAIGPQDFRDQVAIMFPVNTAGAPPFQCMGQSGGTVNIWRWNAEWQKDL
                     GKDSAGIWDVDDQYPGIFWDYYFEEPAGGVTYPDRIGRSLGPFNSGIWSGNIMSDPTL
                     RVSSVEDLNANGFSTLTTQAHQDVIGNGVWEPSGSLKGGGYTGPTWRVVVKRSLETGD
                     ANDTQFKAGASVPIAFAVWDGSNIERNGMKSLSTWFTLKLP"
     gene            19731..21512
                     /locus_tag="PK589_11030"
     CDS             19731..21512
                     /locus_tag="PK589_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ20179.1"
                     /translation="MKVSLLFPPTWHPSQPYLSLPSLTGFLTQAGVKNVSQRDLGIEL
                     LDKVLTQSFAHGLYQQLVEKQRGLERERTGETGPGSAEQLARIIESLDRFPYLFERIE
                     LAKETLRGEGFYDIEAYRNSLFLIDKWLEVLSSLYFPTRMTVVDNQFGDYSIYSSKDL
                     IKAIRDEAQNPYISLFREHVLPSILTDRPDLVGVSITATSQIIPGLTLCRLIKELAPE
                     LHVTVGGSIFTRLVDNLRRCPWLFDLVDDFVVFEGETALLELVNQMDGKRDFSKVPNL
                     IYRQNGKITVNQPFYSENINQLTAPNYDGFPLDLYLSPEPVLPVQFSRGCYYKDCAFC
                     ALTLDHQNFRQRDPGKTVDDLVWLKERYGAQSFFFTDECFALSPTKRLCQQMIDRQVN
                     VKWTCELRFEKNLSRELLTQMRDAGCLKIVFGLESFNQRVMDFMKKGIKQESVRRIVD
                     DCVDLGIAVHCYVIVGFPTEKEEEALETMNFVVGNTKLHESYGFSCQPCLFDLEKEAP
                     IMSDPGSYGIRRIMRPATEDLSLGFFYEVQEGMTPAQAEQLYQQVYEKISEVVCELPF
                     NYSMADGLLYIARAKSQALQIPQPTVS"
     gene            21593..22714
                     /locus_tag="PK589_11035"
     CDS             21593..22714
                     /locus_tag="PK589_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20180.1"
                     /translation="MSAIAAAVERPTGRVDDQGLLFEYTIKLVLFVGFFELVLYRLVS
                     RLGMHISKIAAQHEWIGTVFQLLTSIGFALLNVVAIFVFLALVVLLVNRARARGLTGF
                     NAVTIPSVALLLVLTVAFLIVQPAMLGSIAYNVVTIIALTALMLEYLSQQQEWSKRVM
                     GTVYYFGISGWLYYQIFSTTYGWMGVIAAPPFVYEANRIGEALMVLASILVFWAYGRG
                     VSFRTRNKQQRRRAIWFGAVSAVVFFGLLFMDYFLGLYNPVLAHSIRKAGEGISWIFQ
                     MGMGYTFYLPFAFYVAGLLCWSYTVIRLVTMGRLAGYGLALMFIAGYALLFSSLTLMV
                     ILGVMLLTLDRPKGTVTEQATVSRPPLVGTQESLVGGQI"
     gene            22747..23559
                     /locus_tag="PK589_11040"
     CDS             22747..23559
                     /locus_tag="PK589_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ20181.1"
                     /translation="MDEHTQTGGTEQGSAAVDPGDQPLNPDEEIAEIEKLLSTEPDDF
                     QARCRLGELYFSKGRLDDALTEVKKSIEMAEGLRTEMNRSLAMYYSNLGTIYATKGMI
                     DEAEAEFKRALEVHAYDVLALFNLGRVQADKRKYMESKNYYERLVEITPDDPMAWYNL
                     AGVYVELDNPQVSDYNTMDMAIQCYLRVLELDPKHLEASFKLMEIALNHRKTDLAIKV
                     MESAVEHSPDEPLAYYNLISVYDKCKMFEQAEQARQRLKERFAKKAKEGSAS"
     gene            23584..23880
                     /gene="tatA"
                     /locus_tag="PK589_11045"
     CDS             23584..23880
                     /gene="tatA"
                     /locus_tag="PK589_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNJ20182.1"
                     /translation="MFGSLGFTELILILFIVLIIFGAGKLPQLGEGIGKAIKGFKKSV
                     HEADAIEAEAQAQAQQQAEPVQAQAIAAQPPAPMPTPVVEQQVAAAPPAARAQA"
     gene            23926..25179
                     /locus_tag="PK589_11050"
     CDS             23926..25179
                     /locus_tag="PK589_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20183.1"
                     /translation="METDVQLAPGYIETFAGNGKARSTGDGKRAVKAGIPLPHHVALD
                     REEQWLYFAESGSDRVRRVNLTEGTVHNFAGIGETCYSGDEGPCGEAGLYLPLDVACD
                     SKNDLYVCDSGSNRIRKIDRETGIITTVVGTGEHGFNGDGPALEVNLTWPAAIAFDAD
                     DVMYIADTQAHRIRRFDPRTGRVETIAGSWTAEDEAREQPLVARNLVVLSGDAIGIDF
                     SDDNGWLMPVCSDGLNLSMYLDDGRPALEARLYDIVGIAVDNAGDVYVVDKGSNRVRK
                     IDRQTGLISTLAGVCRYGYDGDGKPAAKSMLHAPEAIVFDQHNHAYISDTGNHRVRKV
                     EAGTGMISTVAGNGDSGYDDKNMGGCGAARFVAKDAAGALKHGDGLLAIEAVVNSPVG
                     LTLDSQGYLYICERGENKIRRAKLW"
     gene            25267..26100
                     /locus_tag="PK589_11055"
     CDS             25267..26100
                     /locus_tag="PK589_11055"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNJ20184.1"
                     /translation="MTSVHTSRTRLLSLLSVLLLLALILGGLGIPIVSSEGMIIRAQA
                     VSGDIPVAPEDPLWQKISPMTLPLSGQIITRPVWPEPTAHALTVRAVHNGTDIAFLLE
                     WQDNTKNDRLTPGTFRDGVALGLPLGDAPAFFCMGQLDHYINIWHWKADWQSDIDRRA
                     SRTTEKDKGGGSEPRRFEVIPRRASSVEDLIGGGFSTLTTKEKQGRVQGKATWKDGTW
                     RVVMRRPLSSEEQENEAKLIPGHIQAVSFAVWNGENKERNGQKAIAPWFQLALDPVTK
                     T"
     gene            26244..26663
                     /locus_tag="PK589_11060"
     CDS             26244..26663
                     /locus_tag="PK589_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNJ20185.1"
                     /translation="MCGAALLLAATVGNPSIAAGSGSNGEMTVEEAIQLGEEFGIAVG
                     EVDEEVQKELKLQRPEGVAVFEVIGNSRADYAGIKVRSVIKEIDKKEVRNLIDFGKAV
                     KVAMKECNFTVGTYEPADPGDPVGWGVNFHFVGCKRD"
     gene            26679..27989
                     /locus_tag="PK589_11065"
     CDS             26679..27989
                     /locus_tag="PK589_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNJ20186.1"
                     /translation="MIGTGRTIQKWAMSGLLMVFALLTVTFHERAIAQKTDGQAPADW
                     VAEIEKIFIRSEDCKQCHDRHYEEWKGMREQTPDLKSFGRVDAALLHGTSFESPVFRT
                     VLGVWMQTNPSLEERQRCLSCHAPAVTVFPQHAEKITAQVLSGKPQVEGIGCASCHLI
                     DAVETAPMPPPTYKVKPSEMLYGPYADPEENLVHPATQLPLFRGANFCTSCHFDKVKD
                     VTQKDLPGEILRGTICQDCHMEPSTGSSTSRRGAMTRSIGRHWFRGVVIPGTLLKNRN
                     LQAEWMPRIDIEATKAATGVEGTVLVKVGSLPHSFPDGDPVLKQFFLSITIKDAQGHI
                     LAEETKHFGLPYEKILRGPIPDPFIKGGNTRRVPFSQTLPAGGEEASTIEVVLNYALI
                     PEPEPELKAKYLATLPTDQERAAAKKVIEEYIQPRMLTYRTKSL"
     gene            28057..29295
                     /locus_tag="PK589_11070"
     CDS             28057..29295
                     /locus_tag="PK589_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNJ20187.1"
                     /translation="MENRRGLLRGLIIMALLMVIGLASYGVQQGLAQDAKSQLTIEKS
                     FPSSGKCKRCHERVFEEWETSPLSKSIHTPTFRAALDAYLTSPAGKDKALCFRCHAPH
                     VREFPDHVQLFVTQAQSGEPSLDGVACAQCHLIKQVDRTKQPPEPKYDLGNKTMYGPY
                     KDFAANLAHQSMESALFHKSDLCLNCHQVVPVAADLGKSNDLLGNWDQSKAVKSGKEC
                     QTCHMPEQVGESANGEAKRKVANHTFPGRIGQLRQEAAKLDVATKVEGDKTSVTVAVQ
                     SLVPHNLPTTHPGWASVVLELTVKGKNLKTVFSDKRVYGRTYADAKGQKTVFDFEAVK
                     VLEETVLKPEETRVEAFSFPTPKDTKTFDVEAILSYAPVTGPASFLQRIEAESSKGAQ
                     DPVFQPIPIAKFSENVPVAK"
     gene            complement(29393..29647)
                     /locus_tag="PK589_11075"
     CDS             complement(29393..29647)
                     /locus_tag="PK589_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20188.1"
                     /translation="MGFAPEYILIDQQKFSKAKLSLDNHVYKNCEIDDCDVYYSGGQY
                     ELIDTHITNSRLILNHPAKGIYSAIQIFKMKSPGSSIISE"
     gene            29988..32042
                     /locus_tag="PK589_11080"
     CDS             29988..32042
                     /locus_tag="PK589_11080"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNJ20189.1"
                     /translation="MSDSAIVTFALVAAVAGIAYGLYLAMWVFKLNAGNTKMQEISKA
                     IQEGAGAYMNRQYKTVGVVAAVLFVLLWGAGAVSDKFGMLTAVGFLIGAGASALAGYV
                     GMIIAVRANVRTAQAAHNGMNAALTVAFRGGAVTGLLLIGLGLLAITSFYTLASQLAG
                     QEKAIHALLSLGFGGSLISVFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVI
                     ADNVGDNVGDCAGMAADLFETYAVTTVAAMVLAFTMFKGVNAPILYPLALGGVTIFAT
                     IIGILFVKVSPGGEIMPALYKGLFVAGGIAAVAFFPITSQIMEGVGGVSGISYYLAAL
                     MGLAVTLALVFITDYYTSKAYAPVKDIAKASETGHATNIIAGLAVGMQSTAAPVVVIG
                     AAILASYWICGGAASGGLYGVAVAAVSMLSMAGIVVAIDAFGPITDNAGGIAEMAHLG
                     KEVRDITDPLDAVGNTTKAVTKGYAIGSAGLAAVVLFAEFAREVAKGTQASTFDLSNP
                     SVLVGLFLGGMLPFIFGALCMKAVGQAAGLVVEEVRRQFRTIKGIMEGTGKPEYGTCV
                     DIVTQAAIAKMMIPGLIPVVAPIIVGVILGPQALGGVLVGSIVTGLFVAISMTSGGGA
                     WDNAKKYIEEQGLKGTDTHKAAVTGDTVGDPYKDTAGPAINPMIKVINIVALLIVSLI
                     VK"
     gene            32227..32424
                     /locus_tag="PK589_11085"
     CDS             32227..32424
                     /locus_tag="PK589_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HNJ20190.1"
                     /translation="MEKQERRPESRKESHAKEEVKPNPKVVESGKKMKEDIDKLVDEI
                     DDVLEKNAEEFVKNYVQKGGE"
     gene            32627..34333
                     /gene="recJ"
                     /locus_tag="PK589_11090"
     CDS             32627..34333
                     /gene="recJ"
                     /locus_tag="PK589_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNJ20191.1"
                     /translation="MLEKLWVFRPFDTVLQNSLVRTLSISPVTASILLARGVTTADQA
                     SRWLSSPQDGLHDPFLIPDMEAAVERLHVALAREERVCFYGDYDVDGVSATSLYLSFY
                     RGLGGNACGYIPHRVREGYGLNEGAVRKLAQEGVTLLVTSDCGTTSHHEIAVAKELGM
                     DVVVTDHHQTDATMPAALAVLNPHRRDAIYPFKGLCSAGLAYKVVTAYQRRYGSGEVD
                     PESCLDLVALATVADIVPLQDENRILVREGLIQINRGARCGIRALKQVAGITKACTSE
                     TIGFRLGPRLNAAGRLDHAMLCVQLLTTESDREAQQIAEQLEQLNRQRQQIEEEITAQ
                     AGASLQQDEPAAAIVLGSRHWHLGVVGIVAARLVDRFHRPSIVIAVDEKGLGKGSART
                     VEGFDLYQGLSACRDLLEAFGGHPSAAGLTIQESRLEEFRRRFCEVAAQWATASLVRP
                     TLHVDAEVKLTEVNFDLIQELESLHPFGAGNPEPTLAVRGLDVVEARVVGEKHLKLRV
                     RQGRSFIFDSIGFRMGSFEELGLKTGRPVDLAFSPERNHWNGYDRVQLRIKALRMSGE
                     VS"
     gene            34336..36519
                     /locus_tag="PK589_11095"
     CDS             34336..36519
                     /locus_tag="PK589_11095"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNJ20192.1"
                     /translation="MPHETVTELDQLIERVKSYNVEADLDLVRRAYDFSAKAHEGQMR
                     RSGEPYFRHPLAVAGLLTHLKTDVTAIVAGLLHDTLEDTLATPLELEQRFGKDVVHLV
                     DGVTKIGKITFRNYEEKQAENFRKMVLSMADDIRVVLIKLADRLHNMRTLEYLSEGKR
                     RQIAQETLEIYAPLANRLGIGWIKNELEDLCLKHLKPDVYEMLRVRVAKRDEDRQQYI
                     HEVIDMVKKAMAEAGLYGEVSGRPKHLYGIYQKMEKQSISFEEVYDLAALRIITDIKM
                     NCYALLGVIHSLWRPLPGRFKDYIAIPKSNMYQSLHTTVAGPKGEHVEFQIRTEEMHR
                     VSEQGIAAHWKYKEHGRIDEKDGKVFSWLRQFVEWNQDLPDNRQFMDSVKLDLFHDVV
                     YVFTPQGMVKELPKGSTPVDFAYSVHTEIGDHCVGAKVNGKIVPLKHMMESGDMVEIL
                     TAANQTPHRDWLKFVRTSRAKTKIKHWIKAEEQSRSIEIGKRLLEAEFRRHGLAPAQM
                     MRSEQLLAVAKQVGYETAEELAAAVGFGHVPTSQVMSKIAPPLPGEAQTVAPEAPVPH
                     KPVIGRTDDTGVQVKGARDLLMQLSRCCNPVPGDRILGYITRGRGLTIHTIDCPNLEA
                     LDYDKNRLVEVEWDQSTPSTHSVKVSVIAVDKTGVLAHVSTAISECQANISRAEITTR
                     EDRKAMLDFIVEVADTAHLSRVFKAIEKVEGVITVRRMRSWQERA"
     gene            36568..38085
                     /locus_tag="PK589_11100"
     CDS             36568..38085
                     /locus_tag="PK589_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNJ20193.1"
                     /translation="MTLGSLAGSILAILACAAGVLGALTYVFTRRGKPVPVLPFYAGG
                     VGVFFVVASLIGHSGLGTSVPHFIQSCLNVAAWLGAAFFFFTILDVLIIGEWLIERGQ
                     RYIPDIVRQLLIGAEVVAAGVLILWLVMGINLVALVALPTVAAAMVGVALKDTLTRFF
                     SGIELGKIVKVGDWVTVLDREGVVTHIGMEHVTLLTRAHDVVSLPNDLVIQSGVTNFT
                     RPTTTHFCNVFVDAAYRTPPEQVCATLLETASSVSGVLPDPKPSAFVTAFNESAIQYR
                     IKFPIGDFSQRDSIESAMRTYIWHAFARTGIEIPFPQRVVHQSANGQDAAQDRYAIER
                     IMAKLTAVDFLATLDTKQIEVLAEGARGELYLPGERIVRQGDPGEVLYVIVSGTADVR
                     LEQGGLTTTVSTLEPGKFFGEMSLLTGEPRSATVVAVTQLSVIAVSKSALLQVVQEDR
                     RLIERIGEVVARRQAVTAAAKAQLSRDAAALSVVTHTRSLVERIQSFFWGTRKST"
     gene            complement(38104..38613)
                     /locus_tag="PK589_11105"
     CDS             complement(38104..38613)
                     /locus_tag="PK589_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNJ20194.1"
                     /translation="MTQSMDHSSQTPHSTWLSRLTSCLWSLAIVASPWSGWAADLPTE
                     TLIAVKMPNGTVIQAELADTALKRAQGLMFRERLADDRGMLFIFGDAQPWTFWMKNTK
                     IPLDIIWMDAKKTIVHIERNTPICTRQDDGCPQYHSEEGALYVLELGAGRAAALQLQR
                     GVKLSFKQP"
     gene            38752..38937
                     /locus_tag="PK589_11110"
     CDS             38752..38937
                     /locus_tag="PK589_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20195.1"
                     /translation="MKETRRVVYMRGVFVCPKCRQSYVQEKWIEEFRIRCHKCDYRGT
                     LTQVSVEEHMDEEVIRR"
     gene            39070..39690
                     /locus_tag="PK589_11115"
     CDS             39070..39690
                     /locus_tag="PK589_11115"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNJ20196.1"
                     /translation="MVQMWRLLLLCATSILIGPTIGSSADSPPITQVLVAYHSLSGNT
                     ERMAAAVAEGARTVPHTEVIVRRIGSVTAEELFAADAIVLGSPVYWSNMAGEVKAFID
                     DWQFKFGVFPEFKLKNKVGAAFATGGQISSGKELTMLSILAAMLGNYMITVSGGGAFG
                     ASATTEGNSPGIDEQELGSARELGRRVADVAGAVKRGFALQERGVR"
BASE COUNT         8712 a        10318 c        12259 g         9158 t
ORIGIN      
        1 ggatactatc ctcggacatg ggactaattc tgaatcttac aaacaacctg ggagaacagg
       61 tggtgaggtt aacaatgagc aaacttcgta tgaacgtaaa agcatctccg atcatcgggg
      121 ctgctcttgg tgcccttctc ctatccgcaa gcgcggttgg gattggccaa gcggcagatc
      181 ttcctgaggg tttcgcaaag ggggatttgg ccccggcccc gcccgcagat atgattgaag
      241 cgggaaagcg tgtgtatttc accaagtgcg tgtggtgcca tggcgtggat ggagccggcg
      301 atggcccggg tgcagatcgg ctttggccgc gtccgagaaa cttcaatcag ggaacattca
      361 aaattcgcca tacggcgagt ggagaattac cgcttttcaa ctacaatgaa aagacccctg
      421 actccggcaa aaacgacttg ttcgatacgg ttactcacgg tctccccggc tctgcgatgc
      481 caccgtggga aggtatcttg accgaagaac agcggattca agtgctgtcg tttgtgacta
      541 cgcaactggt gaaggaccgc aagtttacgg acaagcagtc tgagacccaa accattcttc
      601 aactcggtac gattaagcca gccgaggcga ctgaagatag catcaagaag ggtgcacagt
      661 tgatcgtgga taagaagtgc attgaatgtc acggcgtcga aggccgcggt gacggcaatg
      721 ccttcaacct caaagacgac tggggattct ccattcagcc ggccaattgg cacaagtgct
      781 ggaacttccg tggaagccgt caggatccgt acaatgttcg gaacatcttc cgtaccttct
      841 ccactggcgt caacggaacc ccgatgccgt catttgcgga cagcacgtcg atcgaagatc
      901 gttggaacat tgcgaacttc gtgaattctt tgtgcgaacg cgatgaggaa gggaagcctc
      961 taggtatcga tcctctgact gacaagccga agatcaactt cgtcattcca tccagtccag
     1021 tagaaggcga aatttctagc gatcctgaga atgaaatgtg gaagaagcag ggtcgtcggt
     1081 atgtggccat gggtggtcag atcactcata agccacgcaa ctttgtgaac cgtatcgacg
     1141 acatctgggt caagtcactc tacaacgaca agagcgtggt gtacttgatt cagtgggatg
     1201 accggacgaa gagcatcgcg gaaggcaagc ttccttgggc tccgacccaa gttaacgtcg
     1261 aaaacttcgg cgtgaaagag caggctccaa agaccggcga agaaggttcc atcgcagcgg
     1321 cgcagaataa ctatgccgtc tataacgatg gtattgcatt ccagttcccc atcaagtggc
     1381 aggaaattcc tgctcccttt aaaccacgct atttgttcgg cgatgcgaaa ttcaatgccg
     1441 acatcttgaa gtgggaagcg gacggttcgc tgcgttcctt caagggcacg ggatgggatc
     1501 aggattttga agagcgtgat gacttcgaag agaaggttaa gcttttgaag tcagagtgga
     1561 aaaacggtca gtggaccgtc atgatctccc gtccgctcaa gggtagcaag gatgattatg
     1621 acgaatacac ccggtttgat atcggtaagt acatcccgat ggtattcttt gcctgggatg
     1681 gacacaatgg tgatgcaggt cgaaagatgg cagtctctgc attctactac acgattctgc
     1741 agccaccgat tcctcaggaa gtgtacattt acccggccgt tattgcagtt ggcgtcgtgt
     1801 tcttggaggg ttggatgttg acccgccgcg ccaacaagaa gaagggcaag gtcctgtaag
     1861 cggtaaaagg gtaatgtagc tctcataagg cgagggggtg aggaaactca ccccctcgtt
     1921 gttttttagc tgttgcatta gatgtgagaa gtgggtccga tgatagatga tatttcaggt
     1981 gtcttagtcg ccggcggtcg aagtaggcga atgggacaag acaagcgatt catgaaggtt
     2041 ggtggcgcaa gtgtgtttga acgaacgctg tccgtcttaa gggttacctt tgcggaaaac
     2101 attgtagtgc tcgcggagcc gatagagggg cttgatgtgc agaggtgccc agttgtctat
     2161 gacctgatcc caaacgcagg tagcttaggt ggcctgtata ccggtttgat gaccgcatca
     2221 cgatcgaggg tatttgcctt agcgtgtgac atgccgtttc tagaccctga agttatccga
     2281 ttcatgctgt cccaggatga gtcggccgat gtgatcgtgg ctaatttggg aggtcgattt
     2341 cagccaatgc atgcggtgta ttcgaagcgc tgtgtcccgt ttctccaggc gatggctcaa
     2401 cgaaatgaac tcaagattca aaacctcttt caccagaacg ccctgcgaat gaagatattg
     2461 acagagaatc atctccaggc attcggacca gggttccgtt cgttccagaa tatcaacacg
     2521 ccgagtgagt tggcttctgc cgaagcatcc ccttctattc acccgtgact gctcgtccct
     2581 ataatcgccc gcctctaaga ggggtgttgg tcattcaccc aggggctttg ggcgatgtgt
     2641 tgttagcccg tcctgttctt cgcgcgttgc gttgtcaatt ccctcaacat gaaatcgcac
     2701 tgatagccgc caatgctgtg ggatctctgt tgtgccacag cgcagagctt gatcgagcat
     2761 tctctcttga ggaaaattac ctgagccagt tgtttgctgg cttcgagagg ctgagtgatt
     2821 cattcaggac ttggttgggg cagtgcgagt tagtggtggg atggctgagc gatgccgatg
     2881 gtgccgtgac caatacgttg cgcctcgcag gggtacagca gattcagtta cagactccgt
     2941 tttcttcggc cttagcgaca actcatcaag cagatcgcta ttgggaagca gtcggaatgg
     3001 agggacagcg ggaagacttc acctcatcgc taatgttgcc cgccgaactg gaaaggcagg
     3061 gccaggatgt cttgcaggca tatgactggg acggatcgtc accattgatc ctggttcatc
     3121 cgggaagtgg tagccataac aagtgtatgg atgcgtggcg ccttgcaaag gtcattgagt
     3181 ggttgatcga ggcgcgtatg gctcccatgc tcctggaagg gccagctgat tgccgggccg
     3241 tggcgcaggt gctttcatct ctgactgtgc cggtgcccgt cattcgcgag ctcagtttgt
     3301 ctgtcgcagc cggagtcatg gcacaggcaa agctctatct tggccacgac tcgggcatga
     3361 cacatcttgc tgccgcgtta gctgttccca ccatagcgtg ttttggtcct actcatgctt
     3421 ctcgctggtc tccgcagggg cgcgcggtag cggtcatttc cggtatgcca tgcgtttgtt
     3481 ctgattggag caaggtggag acttgccacg acaaggtgtg tctgcacatt cctgtggaaa
     3541 gcctgattga agcctgccgg agtcaattgc tcaggttgtc atagtccttc tcgacgatct
     3601 cctcatcttg tctctcacat tctggtgtgt tagattgcga gattaatttt cgctgtttcc
     3661 acgcagactt aggggagttt ttgtggctca gggtgtggtg caagataggg tagcagacgc
     3721 gttggcagga gcgcttcgat cggcacaaca acgcgggatt ctcaagatcg agcagatgcc
     3781 gactattaac ctcgaggcgc caaagcggcc tgagtggggc gatgtgtcct gcacggtggc
     3841 catgtctttg tcggcttccg agcgacggcc tccatttgaa attgctcaaa ttatcgcgga
     3901 tcacattcag gatcgagatg cactgttctc gcgggtcgat atcgcgcggc ctggatttct
     3961 taatctcacg ctccaacccg atctctggat ggaagttctg cgacatattg aaacccagcg
     4021 ggccgagtac ggtcacagcc gactggggca gggccggcgc gtattggtcg aatacgtcag
     4081 cgccaatccg actggtccct tgcatgtcgg ccatgggcgt ggagccgccg tggggcaggc
     4141 gctcgttcgt ctcctcaggg caaccgggca tgaggttgtc agcgagtatt atatcaacga
     4201 tgcgggccgt cagatgaaat tgctgggggt gtcagtcctg gcccggtacc tcgaatcgtg
     4261 cggacaggca gttccctttc ccgaagacgg gtatcaaggt gagtatattc gggccgtcgc
     4321 tgcgcgtgtg aaggccgagc agggagtaac gctgctctct ctttcctcga gtgaggcaga
     4381 gcagcgaagc aaggatttcg cctatcggga acttctggcc ctgatacggc aggatttgga
     4441 aacctttggg attacgtttg agtcctggtt cagtgaagct tccttgttgt cctctggagc
     4501 cgtcgagcag gttttgagcg agctgagaag tcgggacctt ctcttcgacc aggatggcgc
     4561 gcaatggttc cgctcgtctg cctatgggga tgaaaaagat cgggtggtgc gcaagcagga
     4621 gggagattac acgtacttgg cctcagacat tgcctatcac cgcgacaagc tgcggcgtgg
     4681 gtttgatctc ctggtcgatg tctggggagc cgaccatcac ggatatattc cgcggatgca
     4741 agcggtggtg caggcctacg ggtatcccaa ggaacggttg cgcgtggtgc tggtgcagat
     4801 ggtcaacctt ctccgcggcg gccggaaagt ggaaatgtcc aagcgagccg gagagtttat
     4861 tacactccgc gaggtcatgg atgaagtcgg ggcggacgcg gccaaatttt tctttctcat
     4921 gcgcgactcc agcacgcatt tagattttga tctagagctg gccaagcagc agtcgcagga
     4981 gaaccccgtg tattacgtac agtatgcgca cgctcggatc agcagtctgt tgcgcgtggc
     5041 ggcatcacga ggcattgagt gtccgcttcc gagtcaggct gacctcaaac tgctgaacga
     5101 tcccgacgaa ctcgcgatca tacgaaaact ttcggtcttc ccggtggtgc tggaagcgag
     5161 cgctgtcgag ctggagccgc atcggatggc ctactatctt cgtgagttgg cgggcctggt
     5221 gcatcctttc tacaacaaac accggatatt gcctccgatg accgacctcg acgcgactcc
     5281 ggcggctcca accgagccgt cggcttctac cagtcgggtg cccgaggcgg tgtcgcccgg
     5341 gctgacaggg gctcgcctgg ccctcatgtg gtcggtgcag caggtggtcc gcaacgggtt
     5401 gacggtgctg ggtgtttcct ccccagagca gatgtaggct gccaatcgga tgctatcgtt
     5461 tcgcattacc catgaagaag caggcgggcg ggcgcgggtc ggggttctca ccaccggacg
     5521 tagcgaagtg gcgacgcccg ctttcatgcc ggtcggttcg ctgggcaatg tacgtagtgt
     5581 tgacggtgaa gaactgctga agatggggta tgggctgatc ctgaacaatg cctatcatct
     5641 ctatctgcgc cccggccaca aagtcgtgga ggagttgggc ggcgttcatg gcttcacggc
     5701 ttggcccggc gccattctca ccgacagcgg tggcttccag gtctttagcc tggcaaagtt
     5761 ttgcaaagtg acggatgagg gcgtggtctt tcaatctcat atcgatggat cgtcacgatt
     5821 catcagcccc gaaacgtcga ttgagattca ggaagcgttg ggcgcggaca tcatcatggc
     5881 cttcgaccac gtcgtcgctc tgccctcctc gctcgaccag gtgcgtgccg ccgccaagcg
     5941 cacatctttg tgggcgcgcc gctgtgtcga ggccaagcgt cgtactgatc aatcgttatt
     6001 cgggattgtg caaggcggat tggaccagag cctacgagtg gagtctgccc gcgatctggt
     6061 gtcggtggga tttgacggat atgcggttgg cggcttgtcc gttggcgaag acaaggccga
     6121 catgtatgcc atgttggatg tcacggtccc cgaactgccg tcggccaagc cccgctattt
     6181 gatgggtgtg ggcatgccag aggacttggt ggaaggcgtc gcgcgcggga tcgatatgtt
     6241 cgattgtgtg gtcccgtccc gccatggccg aaccggatgg ctgttcacct ccttcggacg
     6301 tgtcgtgatc aaacaggctc gatatgcgag ggatgaacag cccatcgatc cggcctgccg
     6361 gtgtccggtg tgtacccgat atacgcgcgc ctatctccat cacttgttcg gcgtgaaaga
     6421 gatgttgggc gcccgcctca atacgattca taatttgtgg ttttttgcga agttgatgga
     6481 agagatccga aacgccgtgc ggagcggcac ctttcaggag tttcgccgtg agtttcaccg
     6541 gacctatgtt gtggaccggc cgggcggcga agggcggtcg gacgtagaca atacgaacat
     6601 gtcgtaaggc gacaagcagg gggctgtatg tgggattcgg tggcctgggc gcagggaacg
     6661 tcgggaaacg cgggagcggg gggaggcctg ttatcgctgg tcccgttcgt gctgatcttt
     6721 gtcatttttt atttcatgct gattcttccg caacaaaaac gccagaagca ggcgaaggcc
     6781 atgctggaga ccttgaagaa gggtgacaag gtggtgacgg cctcaggcat ttggggcacc
     6841 atcacgaacc tgggaaaaga gacggtgacg ctccaaattg cggatacgac caaaatcaaa
     6901 atccaaaaag agcatattgc tcggctgcgc ggtgaagaag aagactgacg cacgcgacgg
     6961 agacgggaag gattggcgca cgtatgaaaa aagtcggcgg acggttgtta gccttagtga
     7021 caatggtggt cgcttcgatc atctttttct tgccctccta ccaacccctc tataaggaat
     7081 tgccgcggtg ggtccgcgag gtgttgccgg ataagggcat tacgctggga ttggacttgc
     7141 aaggcggcat tcacctggtg ttggaagtag aagaagatcg ggctgttgag attgcggtgg
     7201 aacggacggc ggcgggactg caagatctcc tcgtcgaaaa gaaaattccg gctgaatcgg
     7261 tcaaacggac gggatcttcg caaatcacca tcggatttca aaatgccgaa ttgaaggccc
     7321 aggtacagaa gctcctggat gagttcccta cgtatgtgga gaacgagggc gcgggctctg
     7381 ggaacaaagt cgtgtgggaa ttgcgcgagg ccgagattaa gcgcatcaaa gattccgcca
     7441 tcaatcaggc cctggaaacg attcggaatc gcatcgacca gtttggtgtg gcggagccgt
     7501 tgattcagcg gcaggggttg aagcaagtcg tcgtgcagtt gccaggcatc aaagacgcca
     7561 aattggcaaa ggacctcatc aagcagaccg cactgctcga attcaaactg ctggatgacg
     7621 acaatcaact caagctggat ttgccgggcc ggatccaaaa gggtaaggaa cgtgaagagg
     7681 cctttctgaa gcaggttgaa gcaaaggttc cggaaggcga tcaaattctg tttgagcgga
     7741 tcgtcgaaaa ggagagtggt caagaatggc tggcccccta tctggtgaag aaacgggtca
     7801 tgctggcagg cgatgtgctc agcgatgccc gggtctccat cggccagttc aatgagccgt
     7861 atgtgtcggt gacgttcgat gcaaaggggg cacgggagtt cgatcggatc acgggcgaaa
     7921 acgtcaagaa gcgtatggcg atcgtgctcg ataacacgat ctattcggcg ccggtcattc
     7981 aggagcgtat cagcggagga agagcccaga tcacgggaac gttctcgatg gaagaggcca
     8041 acaacctgtc gatcgtactt cgggcgggcg cgctgcctgc gccgctcaag atcattcagg
     8101 atctgaccgt cggtccttcg ctcggacagg actcgatcga gaaaggcatc aaggcgactc
     8161 tgttcgcggg gcttctcgtc atcgtattca tggccatcta ctaccggctg tccggggtca
     8221 tcgcgaattt cgcgttgatg ctgaatctca tctgtttgat cggatctctg gctgcgttga
     8281 atgcgacgct gaccttgccc ggcatcgccg gtattatttt gacgatcgga atgggtgtcg
     8341 attccaacgt cttgatcttc gaacgcatcc gtgaggagtt gcggcaaggc aaagcggtcc
     8401 gggtggccat cgatgccgga tacgataaag ccctcttaac cattgtcgac tcgcacgtca
     8461 cgacattgat caccggtttt gcgctgtttt tgttcggcac cgggccgatc aaagggtttg
     8521 cagttacgtt atgccttggt atcgcgatca atctgtttac cgcgttggtc ggcaccaagg
     8581 tcattttcga cctctttaat cagcgcaaga aaattgagca gctcagcatt taggaaggag
     8641 ccggtatgtt cgagattttg ggaaagacga atatcgattt tatggggaag cgcacgatct
     8701 cgtttgcgct atccggcatc ctggccttcc tgggtattat tgccgtgctt caaatcgcgc
     8761 gcggggcagc caatctggga atcgattttg cgggcgggac ggcagtccaa ctcaaattcg
     8821 atcaatcgat cagaatcgac gaagcccggc gagcattgga atcaaatggc ctgggatcgg
     8881 ccgaattgca ggaattcacg caggacaata agttgctcat tcgtgtgaaa gcgtcgacga
     8941 ccattgagga gaaggttgcg gaacgggtga tggccgtatt cagcaaggag ttccccggca
     9001 acaagtttgt ggtggattcc agtatggaga ttggtccgac gattggaaag aagctccaag
     9061 aagatgccat gattgccgtg ttgatttcat tcgtggggat cgtgctctac atcgcggtcc
     9121 gatttgagct gcgattcggc gtcgcggcgg cgctggcgac gttccacgat gtcctcgcgg
     9181 tattgggcgt gttttacatt ttggataaag aaatcacctt gctggtggtc acagcgctcc
     9241 ttacgctggc cgggtactcg ttgacggaca cggtggtcgt gttcgatcgg atccgagaga
     9301 atcttcgcac gcgccggcgg gaagacgagg aaacgatcat caatcaggcc atcaaccagg
     9361 ttttgagccg cacgattgtt accagtttga ccgtggtgat cgtgctgatt ccgctggtgt
     9421 tggccggcgc ggaggtcttg cacgattttt cgctggcctt gctcggggga gtcatgttcg
     9481 gtacctattc gtccatcttc gtggcaagcc cattgctgct gctgtggccc ggcagcgcgg
     9541 ggagcctcct gaagcgtcgc taggcctcgg ctcgtcagag ccgggcctag ggccccacct
     9601 cctgtgcgac agggccatta gtcggggtat ccgcacgcat gctgttctgg agccggtcac
     9661 acagcctcca gcggaaaatc atcacagcca ttgtgatggt cggtctcctg ccgttgggtc
     9721 tttctcttgt tttgacgtac ctcgaagaac gccgggcctt gagggaaacc atcggggcca
     9781 acttcaaaga agtcgccgta gaggcggccc ggcggatcga gatgcaggtc acgcgcggaa
     9841 tcaacgaagc gcagcagctc gccgccacac cgtttcttcg cacctctgtc accgaatcca
     9901 atcgcactta cctggacaag gacgagaaga ccgtccagtc gatgatcaaa gcctggcagc
     9961 agcgctggcg gcaacgggac aaacaaagcg agtttccgct gttcatcaac cgcatcgtga
    10021 ccaattattt gattcgctgg cacgatatcc ggaaaacgga ctacgtgggt attttcgtga
    10081 ccgacaaccg cggcgcgctc gtggtcagtt ccattccgca ggtcgagtac tactacggca
    10141 agaccgcctg gtggcaggcg ttcatgaagc ggggaacggg gaaggtgttc gtcagtgata
    10201 tcttcttcga ccctgccttc ggcacgcatg tggtcaatgt gtcggcgcca atcattgacg
    10261 atgaacaaca tacgaccatc ggcgcggtga cggtgctctt gcgtcgagat accttgtttc
    10321 actccgtctc tgaggcgacg atcggccaga caggacatgc catgctgttc agttctgacg
    10381 gcgcgccgat gatctgcccg atcctgtctc ccgaagagca tgtggtcaaa cccgagttag
    10441 ttgcagccat caactcgagt gcggccggct ggacgactgc ggagaatgat tcgcatggcg
    10501 gaaaaaattc gatcgtcggt tttgccccgg ttcgcctggg ggctgatctc acacccgaga
    10561 gtctgggcgg taaacgctgg gtgacgtttg tgcggcagga ccctgctgaa tcctacgcgc
    10621 ctctctctcg gctcgtcgta caggtgaccg tctacgggct cggagtcttt gccgtattgg
    10681 tcctcactgg cctgacggtc gctcggcgca tcgcgcgtcc catcggcctg ctgcatgagg
    10741 gcgtgcagaa aatcgggagt ggccgcttgg atcagcagtt ggaccttaag accggtgatg
    10801 aaatcgaaca gcttgccgat gccttcaata aaatggcggc gaacctgcgc gtgtcattcg
    10861 aacagatcga acatcgtatg gaggatgttc gacgtttgga ggcgcggtat cgcgatctca
    10921 tcgagcattc gccggagatg atctatcagc tgaataaagc cgggcaattc gtacatgtga
    10981 ataaaaccgg tttggataag ctggggtaca cgctggagga aatgcttcag atgcgtctct
    11041 gggatctggt ttccaagggg cgtgaggctg aggtattggc gtatctggag cgactcgtcg
    11101 cgcaggggcg tagtacgatt gagacggtgt ttgtggcagc agatggacgt ccgatcgacg
    11161 tggaaattca ttcgacggcg ttattcgaga tcggcggagg gctcgttcac tcccgcgcct
    11221 ttgtgcgcga tgtgacggaa cggcgtctac tggaagaaca aattcaccgc tacaccacgc
    11281 gactcgagca ggcagtcaat gagcggaccc aacagctggt ggcctcgcag gagcggtata
    11341 aagcgctctt tgatctcgtg gccgattctg tgttcatggt gggcgaagac ggcgtgattg
    11401 tcgcagtcaa caagcgtgaa gagcaggcgc tcgggtattc cgaacaacag gtcgtagggc
    11461 acagcattct cgacgtcatc ctgccggctc atcacgagga catgcgttcg ctgttggcga
    11521 agatccactc cggcgaacgg caagtgccca cgcaggaaat cacggtccgt gatgccggcg
    11581 gcaaggaaac gccggtggag atggatgtga tcctggtacg cggcagcgat cacacgtggg
    11641 tgatggtgca gctgcgtgac attacggatc gaaagcgcct tgaacgccag atgcacacct
    11701 accagcagga gctggaagcc aaggtcagcg aacgcacccg tgagatcgaa gagaccaagc
    11761 agtatctgga gaacctgctg gaaaatgcca atgacgtcat ttataccctc gatagtgagc
    11821 agtgctttac ctatgtcaac agcaaggtat tggcctgggg gtacgcgaag gaagatttgc
    11881 tgggacggcc atatttgggg ctgttgtcga aacgtcatcg tgggcgccgg ttaaaatcga
    11941 cgttggatat cggcgtgaag caggtctatg aggtcgaagt cctcacgaaa tccggcgagc
    12001 cccgttctgt catggtcagc gtatcgccgc tgcatggagt cgatggcgag attttggggg
    12061 tcctgggcat tgctcgcgac atgaccgaca ccaagaaact cgagcaacaa atcagaaact
    12121 ctgaaaagct ggcatccgtg ggcaagttgg cggcaggggt ggcccacgag atcaacaacc
    12181 cgttaggtgg tatcctgaac tgtctctaca atatgcgtaa gggcacgctg tctccgtcac
    12241 gccaggaaga atatctcgcg tcgatggaag atggattgcg tcgcgtacag cggatcgtcc
    12301 ggcaactcct cgacttttcc caacagcaca atccggagct ggcactggcg gatatcaatc
    12361 aggtcgtcaa ccgcgtgttg ttgttaaccg accacctgtt cgtgcctcat cgggtgcagc
    12421 tggagacgga cctagccgtc gacatgccgg aaatcatgat cgatcggcat atgatggaac
    12481 aggtgttaat gaatctcgtt ttgaacgcca ttcaggccat gcgaaacggc ggggtactga
    12541 ccattcgcac gacggtcaaa gaggctcatt gtctcgtgcg cgtccaggat tccgggtgcg
    12601 gcatttcttc gtccgttctg ccgcgcattt tcgacccgtt tttcaccacg aaaaacgaag
    12661 gtgaagggac cgggcttggt ttgtcggtga gtttaggcat cgtcgagcgg cacggtggac
    12721 ggattgtagt agagagtgaa gtgggaaagg gcacgacctt tacggtgtcg attcctctct
    12781 cgaccgaacg ctacgccatt gggaggtttt cgtgaagggg ctcaagattt ttattgtcga
    12841 tgatgaaccg ctgatgcggc tctctatgca ggatgcgctg gaaggggtcg ggtgcgaggt
    12901 catggccgcc gcgactggca cggaaggggt ggctgtcctg ggcacgcgcc agtttgatat
    12961 cgtgattacc gacttgcgtc tcccgggcgc tgacggcttg acgattttga agacctgtaa
    13021 ggaacgaagt cccacgaccg aagtcatttt gattacggct cacggatcag tggacacggc
    13081 ggtgggggcc atcaagcttg gcgcgtacga ttacattacc aagccgtttc agatggacga
    13141 attgctgctg atcgtcgagc gcgtcggcaa gattatcggg ttgcggcggg aaaatctcga
    13201 actgaaagaa gtgttggagg atcgtttcag tttcggtggc atccacgggc gcaatcatca
    13261 gatgcgggcg ctgctggaca agatcaagct cattgcggcc gctgattcgc cggtttctct
    13321 tgtcggtgaa cgtggaacgg ggaaggagtt ggtggcgcat accatccatt taaacagccc
    13381 caggcgcgat cagcccttga tcaaaatgtg ttgtgccgac cttccggatc atctgctgga
    13441 ggcggagctc ttcggccatg aaaagggggc ggtacccggt gcctcgcggc agcgacgggg
    13501 acggttcgaa ctcgcacata aggggaccct gctgctcgac gacgtagatt cgctgtctcc
    13561 tctcctgcag agcaagctat ggcgggtcct gcaggaacgg aagtttgagc gtgtcggagg
    13621 gcgtgaatcg atcgagctcg atgttcgaat cctgtgcgcc tcgcggcagg atttgaagga
    13681 ggcggtagcg cagggacggt tccgtcagga cctctgtgat caccttgctg ccgttcaggt
    13741 ggttgtgccg cctttgcgcg agcgtcggga agatgtgctg gtcattgccg agcacgttct
    13801 tgagctgcga tcaaaaagcc tcaataaatc ccccaagggg ttttctcagc ccagttgcga
    13861 cttgcttcta cactattctt tcccgggcaa tgtcggcgag ttagagcaga tggtggagcg
    13921 ggcggtgacc ttggggcgaa acggggagcc tttgcagccc tgggaccttt gtggattcca
    13981 aagctgcccg tatctcggtg gtgcgcctca ggcgacatgc gggttttgcg acgagggcct
    14041 gtctgcaaag gctgacgaac ctgcggcaca ggccgctacc acattggcga cggcgcggga
    14101 agcctttgaa aaggactaca ttgaaacggt gttgaagcag gtagacggca gtcgcacaag
    14161 cgcagctgcg gtattgggat tgtccaggaa ggccctgtgg gacaagtgca agcggtatgg
    14221 catctcctcc gccaagggtg aggcggagga gagagacgag taaattactc tgccggctct
    14281 tgaccgcctt cccacagttt cacggtgcga tcccaggaag cgctggccag tcgcagtcca
    14341 tcaggcgaaa gagccactcc gcgcacgggg ccggtgtgag ttttatcagt ccggaagttc
    14401 cggcctgtgg cgatgtccca gaaccggatc gtgttgtcgt caccggagct aatcaggact
    14461 ttcccgtccg gtgacacggt cagggagcgc acccagccat tgtgtccgcg caggacgcgg
    14521 agttcgttca tggtcggcat ttcaaaaaac cggatggtat tgtcccggct gccggtaatc
    14581 aggatcttgg catcgggcgt aaaggtcatc gccccgatcc agaactccat cggtttttga
    14641 aatccgccgt catcctcgat gaagaagccg cgcgtttccg tcgaatcttc agccgtttcc
    14701 tcccaatgtc cattatggag gattttttct tcgccgctcg gcaacacttc ccagatgcga
    14761 attttgccga tgtcggagcc actcgcgagg tgcacgccat ccggtgagaa ggccacgcag
    14821 accgaccaat ggtggtcgta ttgatccttg cccagaagcg tcatgagatc ggttccggtc
    14881 gtgacttccc agatcttgat gtctttattg tggctgccgc gggccagcat gaggccatcg
    14941 ggggacaatg acaaactgca cacattgtga tcatagcgag taaacaagag cttggtcggc
    15001 tccccggttt tcgggttcca caggcgaatc gtccggtctt cgctccctga ggccagcgta
    15061 cggccatccg gcgtgaagac cagggctcga atatcggccg tatggccgcg caacgagcgc
    15121 agaagacgtc cggtttcaat gtcccaaatt cgaacgtaac gatccacgcc gccactgacg
    15181 atcgtctgac cgtccggtgc ataggcgaca gtccacacgc catgcgaatg tccgcggaag
    15241 gttttgagtt cccggaagcc tgttttccaa tactctaaat ggtcgatagc cggtgcttga
    15301 gcacttgccc cttgtgtggg cgccggtgcg gccgaaggaa ttgtcggggg cgtggtcgtt
    15361 gaattgctca taaaagtttg tgcctcgtcc ctggtgtgac cgagggccat ctagccctgg
    15421 ccagccctcc gcggtttgca aaaaacgccg aggggtttgt ataatcggcg gtcgttttga
    15481 ggatcgtaag agaaggaccg tgagcaagtc aagcacagtg cttacgcatg gtttctccct
    15541 tcactgtaac ggaaaatgtg aggtcttatg actaccggag tagaagtatc caagccgtcc
    15601 atggaaattc gatttcagcc ggccttactg caggaagtca tcgactcctt tattgaaaaa
    15661 accgagcggg aaggggatcc gacctactac aaggaatttc acgaactggc cgatccgatc
    15721 tatgagaaat ttacgctgga tgatcgtgaa gcggagttca agaaactgta tcaatactta
    15781 ttcgggatct ggggcttttc cgacatcatc cgtgacgcct tcaacgaatt tcccgacctc
    15841 aaggctcgag tgggagtcgt cctggtcaag ggtgtgctga aggaggatca ggagggcgtc
    15901 gacgtgcttc gcaaatgggg atcggtcgag cacgatctgg ccaagcaatt cgaggaaaag
    15961 gggctgaagg gcgtcgggat taagctgatt ccacgccgct tctacgatcc cgcgctgact
    16021 cgctattgcc gtcacgaact gatgcatatc aacgatatgt tggatccggc gtttgggtac
    16081 gatcctgaca ccagggttgg acagaacccc ggcgaggaaa cgctcattct gcaccgctac
    16141 cgcattcttt ggaatatcag tgtcgatagc cgtctgattg ctgcgggcaa ggagccgatg
    16201 cttcaaaagg aggatcggtt caaggaattc cgctcctggt atcgcaaaat tcccccggct
    16261 caattgaagt ctgtgttcga agggttgtgg cagacgagct acttcaccca ttcggagctg
    16321 gtagagatgg cgaccgatac cctccgcatt ttggatcgcg ccgtcgatgt cgaaggggga
    16381 gaaatcccgg agacccagaa caaggtcatg ttaatgccgg ggtttccctg tcccttgtgc
    16441 cgatttccga cgtatacctg ggtcgaggat atggagacga agttggagcc atacattttg
    16501 gacttcattc gtgaaaacca tcctggctgg gacgtcgagt acggagcctg tgaccgttgc
    16561 gtggaagtct ataagttgcg tgccgacggt gtcatgtagt cggggtgcaa catggccggt
    16621 gatcccaaat acgggcgtcg tgatttcctg aaagattctg tggtctcggt ggccaaggcg
    16681 gcgagggaat ttgctatcca taaggatgcg cctcgcgaac agccggcagc ccccgtgcgg
    16741 acagactggt tgcgccctcc tggtgccgtc gacgaatcta ccttcctcga acgctgcaca
    16801 cgctgcagtg attgcatcaa ggcctgtcct cctggggcga tcgtaagcga tctctcgaac
    16861 ggaactccgg tgattttcgc caatcaagtc gcctgcgagc tctgtgagga ttttccctgt
    16921 attgccgcat gtgcgacgga tgcgctcttg ccggtggctg atgttgtcga agtgcggatg
    16981 ggcatcgcca cggtgtctca tcgtgtttgt acggcggggc agggatgcca cgcctgcgta
    17041 tcgaagtgcc ctgttgatgc cctgtctatg gattttcatg cgctccgtct tgacgtggag
    17101 caggcgcgct gtgttggatg tggtatgtgc gagcagattt gtaagactgt caatgaccgc
    17161 attgcggtca aagtcgtccc ggccagaaat ctttctgcgg gcagtcttaa ctactgaatc
    17221 agtcgtgtgt taagaaatgt cgaggcattc atccatcatg ttatttcatg aaacatcggc
    17281 acggaagcaa tggcaaagga gagagagttc atgcttgaca tccctccctc ctttacctat
    17341 catacgcctc ctgtgccgca ggactcagat gttgcgtagt gtgcgcaaaa aatgcgcaac
    17401 atgttgaggt gaggagcaag atacgtatga catcgaaaca agtcgtgcag cccacctccc
    17461 cgtcctccac cgcaattgct ccccccaagg cagttccgat caaggattcg cctgctgtcg
    17521 agcgcgcgct caatcgcagc aagatatatt tgttgttctc ctggagcctg ttgtatccgg
    17581 aggatgagga gtttttggat tacctgcagt gtggtgagtt cgtcgaagac ggacgcgctg
    17641 cgttagacgg gttgcggctc gcgttggacg gcatcggtgg cgatcgtgcc attcagaaga
    17701 ttgccctgat gaagaagcaa ttcgaccaga tcgagaagtt ggtgtcggcg gagtgcgtca
    17761 attggcaaat tggcgatctg cagaccgaac accgtcgggt gttcaccaat gtgatcacgc
    17821 tggattgccc gccctacgaa accctctttg ggaacgacca cgtgttcgcg caatcccacg
    17881 tcatgggtga cattgcggga ttctacaaag cgtttggggt ggagctttcc aaggatgtgc
    17941 atgaacgtct ggaccacctc agtgtcgaac tcgaattcat gcatttcctg acgtataagg
    18001 aatcctattc gcgttgccac gatggaatcg ataaaacgga aatcgtggtc gatgcgcaaa
    18061 agaagtttgt gaaaaaccac atcggccgct gggtgccgct gttctgccgg atgttggcaa
    18121 aaaaatcaga caccgggctg ttcaagctta ttgccgactg tatgtcggaa tggatggatt
    18181 ttgaggtcgc cttccttggg gtaaccgtgc agccatactc cgaggcggac tatagaccag
    18241 ctaccttcaa tgctccggaa ggtcaaacct acgagtgcgg ggcgcaggac aaggggaatg
    18301 agctcagtat gttgctgagc gaagtcggcg ctgagtcctt catggaccag aagacaaaag
    18361 agaaggacgg cgagaagagc gaaggcccag tcgggactgc gtaggagttc tcggttttcg
    18421 ggcagtgcag tattcggttt tcggtagacg ttatacgttc ccagttcttt ttcttattaa
    18481 tctttcagag gagggcatac gcaatgagac tggtgcagac acgcaacaag cgtttggtgt
    18541 ttggcattct gttctctgcg ctgatcgtcg gcattatgtt gacgatcggg caggtgcctc
    18601 tcgcggtcag ccaaccggtg accattcccg tgaaggcgat caagggggta atcccgatgg
    18661 atggtgcaaa ccccatctgg gaaggagtgc caggtgtcat catcccattg agcggacaga
    18721 ccatcacgac cccgatgcac ccgaatattt cggtcaagtc ggtgtttgtg aaggcggtca
    18781 gcaacgggaa ggatttgggg ttgcgccttg attggagcga ccagaccaag aacgatacgg
    18841 cgattggtcc gcaggatttt cgcgaccagg tagccatcat gttcccggtc aatactgcgg
    18901 gcgcgccgcc cttccagtgc atgggccagt ccggtgggac ggtcaacatc tggcgttgga
    18961 acgcggagtg gcagaaggat ctcggcaagg atagcgccgg aatctgggac gtggacgacc
    19021 agtaccctgg catcttctgg gactactatt tcgaagagcc ggctggcgga gtcacctatc
    19081 cggatcgtat cggccgcagc cttgggccat tcaactccgg tatttggtcc ggtaacatca
    19141 tgtccgaccc gacactgcgc gtcagctccg ttgaggatct gaatgccaac gggttcagca
    19201 ccttgaccac ccaggcccat caagatgtga tcggcaatgg cgtttgggag ccgtcaggtt
    19261 ccctcaaggg cggtggatac accggtccga cctggcgcgt agtcgtgaag cgttcgctgg
    19321 agaccggcga tgccaacgac acccagttca aggcgggagc ctctgtgccc atcgcgtttg
    19381 cggtgtggga cggttccaac attgaacgga acggtatgaa gtctctctcc acttggttca
    19441 cgctgaagtt gccgtgatcc aagcggtctg aagtaaaatt cctgcccctg tgccgggctg
    19501 gaacgtgttg agggcttgaa gcagaaggcc ccgagttctt gtcagcgatg gcaaagtgac
    19561 tcggggcctt cgtgcatttc tgggtctctg tgacgcattg gcgcgaacag tggaatttct
    19621 ttttcatcac agtcggaggg ttgctttcgg accaaagcgg agtgtataaa ccgatacggt
    19681 acaaatctcc tgaaaacact gattttcctg cttaatataa agataatttt atgaaggttt
    19741 ctctcctgtt tccaccgacg tggcaccctt cgcaacctta cctaagcctc ccttctctca
    19801 ctggtttcct cactcaagcg ggagtcaaaa atgtctccca gcgtgatctc ggcatcgaat
    19861 tgctggacaa agtcctcacc cagtcctttg cccacggcct ctatcagcag ttggtcgaga
    19921 agcagcgagg ccttgagcga gaacgtacgg gagaaactgg gcccgggagt gcggagcaac
    19981 ttgcgcggat tatcgaatcc cttgatcgct tcccctactt gtttgaacgt atcgaacttg
    20041 ccaaagagac attgcgggga gaaggcttct atgacatcga agcctatcgc aacagcctct
    20101 ttctaatcga taagtggctc gaggtgctgt catcgctcta tttccccacc agaatgaccg
    20161 tcgtggataa tcagtttggt gactactcca tctattcgtc gaaagatctg atcaaagcta
    20221 tccgtgacga agcgcaaaat ccctacatca gtctcttccg tgagcacgta ttgccctcta
    20281 ttttgaccga tcgtccggat ttggttggcg tctcgatcac ggctacgtcg caaatcatcc
    20341 cgggtctcac gctttgtcga ttgattaaag aacttgcgcc ggaactgcac gtgaccgtcg
    20401 gaggcagcat tttcacgcgc ctggtcgata acctgcgacg ctgcccatgg ctcttcgatc
    20461 tcgtcgacga ttttgtggtg ttcgagggtg aaaccgcctt gctcgaactc gtcaatcaga
    20521 tggatggcaa gcgtgacttt agcaaggtgc ccaatctgat ttatcgtcag aatggaaaga
    20581 tcaccgtcaa tcagccgttc tactccgaga atatcaacca actcacggcg cccaactacg
    20641 acggatttcc gctcgacctg tatttgtctc cggagccggt actgccagtg caattttcgc
    20701 gaggctgtta ctacaaggac tgcgcgtttt gcgcgttgac gctggatcat caaaacttcc
    20761 ggcagcgcga tccgggcaag acggtggatg atctggtctg gttgaaggaa cggtatggcg
    20821 cgcaatcgtt tttctttacc gacgaatgct tcgcactgtc gcctacgaag cggctctgcc
    20881 agcagatgat tgatcgtcag gtcaatgtaa agtggacctg cgaattgagg ttcgaaaaaa
    20941 atctctcgcg agaattgctc acacagatgc gggacgccgg ctgcttgaag atcgtctttg
    21001 gtctggagtc gttcaaccag cgtgtcatgg acttcatgaa aaaagggatc aagcaggaat
    21061 ccgttcggcg cattgtggat gactgcgtcg atctgggcat cgcggtgcac tgctatgtca
    21121 ttgtgggctt ccccacggag aaggaggagg aagcgctgga aacgatgaac ttcgtggtag
    21181 gcaataccaa gctccacgaa tcatatggat tctcttgcca gccctgcctg tttgacttgg
    21241 aaaaagaagc gccgatcatg agcgatccgg ggagttatgg cattcggcga attatgcgac
    21301 cggccaccga ggatttgagt ctcgggtttt tctacgaagt gcaggaaggg atgacgccgg
    21361 ctcaggcgga gcagctctat cagcaggtgt acgagaagat cagcgaagtc gtgtgcgagt
    21421 tgccgtttaa ctattctatg gccgacgggc tgctgtatat tgcccgggct aagtcgcaag
    21481 cgttgcagat accacaacct accgtctcct agattggaaa gtcgggccgc gcagagtgcg
    21541 gcctcatgcg gtgtggtcac gaagtgcttc acgtttgcca ggggggctgg agatgtctgc
    21601 tatagccgcc gccgtcgaac ggccaacagg acgagttgac gatcagggat tgttgtttga
    21661 atacaccatc aagctggtgt tgttcgtcgg ctttttcgaa ctggtgctat atcgtctggt
    21721 ctcccgtctg ggcatgcaca tcagcaaaat tgccgctcag catgagtgga ttggaacggt
    21781 cttccagctg ctgacctcga ttgggtttgc gctgctgaat gtcgtggcca tttttgtgtt
    21841 ccttgccctc gtggtcttgc tggtcaatcg tgcccgcgca agagggctca cgggttttaa
    21901 tgccgtcact atccccagcg tggcgcttct gctggtgttg acggtggcct ttctcatcgt
    21961 gcaaccggcc atgctcggct cgatcgccta caacgtcgtg accatcatcg cactcacggc
    22021 gctgatgctg gagtatctct cgcagcagca ggagtggtcc aagcgcgtca tgggcaccgt
    22081 ctattatttc gggatttccg gatggctgta ctatcaaatc ttctccacca cctacggatg
    22141 gatgggagtg attgcggcac cgccgtttgt ctatgaagcc aatcgtatcg gcgaagcgtt
    22201 gatggtgctg gctagtatcc tggtcttttg ggcctacgga cgcggagtat cgttccgcac
    22261 gaggaacaaa cagcagcgcc gccgcgcgat ctggttcggg gcagtgtcgg cagtggtatt
    22321 tttcggcctg ttgtttatgg attatttcct ggggttgtac aacccggtgc tggcccattc
    22381 catccggaag gccggcgagg ggatcagctg gatcttccaa atgggcatgg gctatacctt
    22441 ctatctgccc ttcgcctttt atgtcgccgg actgttgtgc tggtcgtata ccgtcattag
    22501 gctggtgacg atggggcgcc tggcaggcta cggactggcc ttaatgttta tcgccggtta
    22561 tgcgctgctg ttttccagtt tgaccttgat ggtgattctt ggggtaatgt tattgacgct
    22621 cgatcgccct aagggaacgg tcaccgagca ggcgacggtg agtagaccgc ccctggtcgg
    22681 cacgcaggaa tccctcgttg gcggacagat ttaatttttg actcacccag attaggatac
    22741 gttgttatgg atgaacacac acagaccggc ggaacagaac agggtagtgc ggcggtagat
    22801 cctggcgatc agccgttaaa tcccgatgaa gaaattgctg agattgaaaa gctgctcagc
    22861 accgagcccg atgattttca ggcacgctgc cgattgggtg aactgtactt cagcaagggc
    22921 cgattggacg atgcccttac agaagtaaag aagtccatcg agatggccga gggcctcagg
    22981 acggagatga atcgctctct ggcgatgtac tactccaacc tcgggaccat ctatgctacg
    23041 aagggcatga tcgatgaggc cgaggcggag tttaagcgtg cgcttgaggt gcatgcttat
    23101 gatgtcttgg cgctgtttaa tctcggccgg gtgcaggcgg acaagcgcaa gtacatggag
    23161 tcgaagaact attacgaacg cctggtcgag atcacgccgg acgatcccat ggcctggtac
    23221 aatctcgctg gcgtttacgt cgagctcgat aatccgcaag tctccgacta caacaccatg
    23281 gacatggcga ttcagtgtta cctgcgtgtt cttgaactgg atccgaagca cctggaagcc
    23341 agcttcaagt tgatggagat cgcgctcaat caccgaaaga ccgatttggc gatcaaggtg
    23401 atggagagtg cggtggagca cagcccggac gagccgctcg cgtattacaa tttgatcagc
    23461 gtctacgata agtgcaaaat gtttgaacag gccgagcagg ctcgtcagcg gttgaaagaa
    23521 cgatttgcga agaaggccaa agagggatcc gcatcctgaa cacgtgagca aaggagacac
    23581 accatgtttg gcagccttgg ttttacagag ttgatcctga tcctgtttat cgtgttgatt
    23641 attttcggag cagggaagtt gccgcagctt ggtgagggga tcggcaaggc catcaagggc
    23701 tttaaaaaat ccgtccatga agcggatgcg atcgaagcgg aagcccaggc gcaagcgcag
    23761 caacaggccg aacccgtgca ggctcaggcc attgccgccc aaccgccggc tccgatgccg
    23821 acgcctgtgg tggagcaaca agtcgctgcg gcccctcctg ccgcgcgcgc acaagcataa
    23881 tatcctcgat aacaagggcc cagcatagaa cggacgcaca acgttatgga aacagatgtt
    23941 cagttagctc ccggatacat tgaaaccttt gccgggaatg gaaaggccag gagtacggga
    24001 gacggcaagc gcgcggtgaa ggccggcatt ccccttccgc accacgtcgc tctggatcgg
    24061 gaggagcagt ggctgtactt cgcggagtcc ggatccgatc gtgtgcgtcg cgtcaacttg
    24121 acggaaggta cggtccataa cttcgccggc atcggagaga cgtgctacag cggagacgaa
    24181 gggccttgtg gggaagccgg cttgtatctg cctctcgatg tggcctgtga ttccaagaac
    24241 gatctgtatg tgtgcgattc agggagcaac cggattcgca agattgatcg ggagaccggc
    24301 atcatcacga cggtcgtcgg gaccggtgaa catggcttta atggagacgg accggcgctg
    24361 gaggtgaatc tcacgtggcc tgccgccatc gcgttcgatg cggacgatgt catgtatatc
    24421 gccgacacgc aggcacaccg gattcgtcgg ttcgatccac gaacgggccg tgtcgaaact
    24481 attgccggca gctggactgc tgaggacgaa gccagggagc aacccttggt tgcgcgcaat
    24541 ttggtcgtgt tatccggcga tgcgatcggt atcgatttca gcgacgacaa cggttggctg
    24601 atgccggtct gttctgacgg gctcaatctc tccatgtatc tggatgatgg tcggcccgct
    24661 ctagaagcgc gtctctatga tatcgtcgga attgccgtcg acaatgccgg ggatgtgtat
    24721 gtcgtagaca agggcagcaa tcgcgtgcgc aagatcgatc gccagaccgg actgatttcg
    24781 acactcgccg gcgtatgccg ctatgggtac gacggagatg gcaagccggc agccaagtcg
    24841 atgttgcacg cacccgaagc cattgtgttc gatcaacaca accacgccta tatctccgat
    24901 accggcaacc accgtgttcg caaagtcgag gcggggacgg ggatgatcag caccgtggcc
    24961 gggaacggcg atagcggata tgatgataag aatatgggtg gctgcggcgc tgcccgcttt
    25021 gtcgcgaaag atgcggccgg cgcgctcaaa cacggggacg ggttgctcgc tattgaggcg
    25081 gtggtgaatt cccccgtggg cttgacgctc gactcccaag gatatctgta tatttgcgag
    25141 cggggtgaaa ataagattcg tcgcgccaag ttgtggtaac gcttggagga cggagccaag
    25201 gctcccgcac agggaacgcg tgcgcgtgtt gtcacgacta aggccacgcg ccgggacatg
    25261 aggcgaatga cgtcggtcca cacatcccgc accagattgt tgagtctcct ctccgtgttg
    25321 ttgcttctcg ccctcattct gggtggtctc ggtatcccca ttgtcagttc cgaaggcatg
    25381 atcatccgtg cgcaggccgt ctcgggagac atacccgtgg cgccggaaga tccgctctgg
    25441 cagaaaatct ccccgatgac cttgccactg agcggccaga tcattacccg ccccgtctgg
    25501 ccggagccga cagcccacgc gttgacggta cgcgccgtgc acaatggtac cgacatcgca
    25561 ttcttgctgg aatggcagga caatacgaaa aatgatcggt tgacgcctgg cacatttcga
    25621 gatggcgtgg ctttgggact tccgcttggt gacgctccgg cgtttttttg tatgggacaa
    25681 ttggaccatt acatcaatat ctggcactgg aaagctgatt ggcagagcga catcgatcgg
    25741 cgtgcatcga gaaccaccga aaaagacaag gggggcggca gtgagccccg gcggtttgag
    25801 gtgattccac gacgggcctc ttcggtagag gatttgatcg ggggcgggtt tagtacgttg
    25861 accacgaaag aaaagcaggg tcgtgttcag ggaaaagcta cctggaagga cgggacgtgg
    25921 cgtgtcgtga tgcgccgtcc cctctcgagt gaagagcagg agaatgaagc gaagctgatt
    25981 cccgggcaca tacaagccgt gtccttcgcc gtgtggaatg gtgagaacaa agagcggaac
    26041 gggcaaaagg cgatcgctcc ctggtttcaa cttgcattgg atccggtcac aaagacgtaa
    26101 ttcttcctgg taggatgatg taaaaagccg agcgcatcgg cgagagcttt gttcgcaaag
    26161 gtagaggcca gcttttggag gacagtgttc agattcatcg cgtgatgcac aacagagccc
    26221 tttctttcgg aagggctctt tgtgtgtgtg gagcggcgct gctccttgcc gccacggtcg
    26281 ggaatccttc gattgccgca ggctcaggca gcaatgggga aatgacagtg gaggaagcca
    26341 ttcagcttgg agaggagttc ggcattgcgg tgggtgaggt cgacgaagag gttcaaaagg
    26401 agctcaaact tcaacggccg gaaggtgtcg ccgtcttcga ggtgatcggc aattcgcggg
    26461 ctgactatgc gggaattaag gtgcgctccg ttatcaaaga gattgataag aaggaagtcc
    26521 gcaacttgat tgatttcggc aaggcggtca aagtcgccat gaaggaatgt aactttacgg
    26581 tgggaaccta cgagcccgcc gatccaggcg atccggtcgg atggggagtg aattttcatt
    26641 tcgtcgggtg caagcgggat taagagagcg agccacacgt gatcggaacg ggacgaacca
    26701 ttcagaaatg ggccatgagc ggactgctga tggtatttgc gctgctcacg gtcacctttc
    26761 atgagcgggc tatcgcgcaa aagaccgacg gtcaagcccc ggccgattgg gtggcggaga
    26821 tcgaaaagat ctttatccgt tctgaagatt gcaaacagtg ccacgatcgg cactacgaag
    26881 aatggaaggg catgcgggag cagacgcccg acctgaaatc cttcggtcga gtggatgcgg
    26941 cgttattaca cggcacttcc tttgaatctc cggtgttccg cacggtgttg ggcgtctgga
    27001 tgcagaccaa tccttcgctg gaagaacggc agcggtgtct atcctgccat gcgccggccg
    27061 tcacggtctt tccccaacat gccgaaaaaa ttaccgcgca ggttctctca ggcaagcccc
    27121 aggtggaagg gatcggttgt gcgtcctgtc atctgatcga tgcagtcgag accgcaccca
    27181 tgcctccgcc gacctacaag gtgaagccga gcgagatgct gtacggcccg tacgcggatc
    27241 cggaagaaaa tttggtccat ccggcgacac agttgccgct gttccgtggc gcgaacttct
    27301 gcacctcctg ccatttcgat aaggtcaagg atgtcacgca gaaagatctc cccggagaaa
    27361 ttctgcgcgg tacgatttgc caggattgcc atatggagcc atccaccggg agttcgacgt
    27421 cgagacgtgg tgccatgacc cgctcaatcg gacggcattg gtttcgcggc gtcgtcattc
    27481 caggcacgct cctgaagaat cgcaatctgc aggcggaatg gatgccgcgt attgatatcg
    27541 aagcgacgaa ggcggcaaca ggggtagaag ggacggtgtt ggtgaaggtc ggcagcctgc
    27601 cgcatagttt ccctgatggc gacccggtgt tgaagcaatt cttcctctcg atcacgatca
    27661 aggatgcgca ggggcacatc ctggcagaag aaactaaaca tttcgggttg ccgtacgaga
    27721 aaattttacg cggtccgatt cccgacccct tcattaaggg cggcaacacc aggcgagtgc
    27781 ccttttcgca gaccctgccg gctggaggag aagaggcgtc gaccattgaa gtcgtgctca
    27841 actacgcgtt gatcccggag cctgaacctg aactgaaagc caaatatctg gccacgctgc
    27901 ccaccgacca ggagcgtgcg gccgccaaga aggtcatcga ggaatatatt cagcctcgca
    27961 tgctgaccta tcgaaccaaa tcactgtaag accccctgcc ggttctcacg acaacgggca
    28021 gcgaggtgtg caccatgtgc ggaggatatg caaccggtgg agaatcgacg aggtctgttg
    28081 cggggactca tcatcatggc attgttgatg gtgattggat tggccagtta tggcgtgcag
    28141 caaggcttgg cgcaggatgc aaaatctcaa ctgacaatcg aaaagtcctt tcccagttcc
    28201 ggtaagtgca agcgctgcca tgagcgggtg tttgaggagt gggagacatc gcctctctcc
    28261 aaatcgatcc atacgccgac attccgcgct gccctggatg cctatctgac gtctcctgct
    28321 ggaaaagata aggccctctg tttccgctgt catgcgccgc atgtgcgtga gtttcccgat
    28381 cacgtgcagt tatttgtcac tcaagcccaa tccggtgagc cctccttaga tggtgtggcc
    28441 tgcgcgcaat gccatctcat taaacaagtt gatcgaacca aacagccgcc ggaaccgaag
    28501 tatgacctcg gcaataaaac catgtatggg ccgtacaaag atttcgcggc gaatctcgcc
    28561 catcaatcga tggaatcggc cctgttccac aaatccgatc tctgcttgaa ctgccatcaa
    28621 gtcgtgcctg tagcggctga tcttggaaag agtaatgacc tgttagggaa ttgggatcaa
    28681 agcaaggccg tgaagtccgg aaaggaatgc cagacctgtc atatgcctga acaggtggga
    28741 gagtcggcca atggcgaagc caagcgaaag gttgccaacc atacatttcc tgggcgtatc
    28801 gggcagctca ggcaggaagc ggccaagttg gacgtggcga ccaaagtgga gggcgacaag
    28861 acatccgtga cggtggcggt gcagagtctc gtccctcaca atctgccgac gacccatccc
    28921 ggatgggcaa gcgtggtttt ggaactgacg gtgaaaggga aaaatctcaa gacggtcttc
    28981 agcgacaagc gcgtctacgg acggacctat gcggatgcca aaggtcagaa gacggtattc
    29041 gattttgagg cggtgaaggt ccttgaggaa acggttctca agccggagga gactcgcgtc
    29101 gaggccttca gttttccgac acccaaggac acgaaaacgt tcgatgtcga agccatcctc
    29161 agctatgctc ccgtgaccgg cccggcgagc tttctgcaac gtatcgaggc cgagtcttca
    29221 aagggtgcgc aggatccggt gttccagccc attccaatcg cgaaattcag cgagaatgtt
    29281 ccggttgcga aatagctgtc tggcgttgag cgtgaaatgg ggaaacggct gagaagcggg
    29341 acgagagcca ctcaggcttc gatccgctga gtggcgtctc acgtgcaacg aattattcgg
    29401 agatgatact ggagcctggc gatttcatct tgaagatctg aattgcgctg tagatccctt
    29461 tggcaggatg gttcaagatc agtcgcgagt tggtgatatg cgtgtcgatt agttcgtatt
    29521 gcccgccgct ataatacaca tcgcagtcgt caatctcaca attcttgtag acgtggttgt
    29581 ccagcgacag cttggctttg ctgaacttct gctgatcgat caggatgtat tccggcgcga
    29641 atcccatgtt cgtcctccac agcccgcgcg cactataaca aactggatat ccttgcgcaa
    29701 accgcctgcg tgctgccgtt ccgcctcttg ctgcatgatc tttcgccttg atcagcctcg
    29761 atcaccccta tagaatggcg ccacttttcc tctcgttcgc gaccgtgtca gttgtcgggc
    29821 atgcggcggt attcctgctg cacaatcagt ccgcgatggg ctcaagccac ggagcacctg
    29881 tgacattcat cgatccttac atgtgagtga gcttttcacg gcgaatcgat cccgtaccac
    29941 gtcagctgga ttctcagttt caccatctgt aacaggagga gaacactgtg agcgactcag
    30001 cgattgtgac ctttgctctg gttgcggcgg tggccggtat tgcctatggc ctgtatctcg
    30061 ccatgtgggt gttcaagctg aatgccggca acacgaagat gcaggaaatt tcgaaagcga
    30121 ttcaggaggg agcgggcgcc tacatgaacc gtcaatacaa gacggttggt gtggtggctg
    30181 ccgtgttgtt tgtcctcttg tggggcgcag gagctgtatc cgataagttc ggcatgttga
    30241 cggcagtcgg attcctcatc ggcgccggcg cgtcggcgct cgccggatac gtcggaatga
    30301 ttatcgcggt tcgagccaat gtgcgaacgg cacaggcggc gcataacggc atgaatgctg
    30361 ccctgacggt ggccttccgc ggaggtgcag tcacgggatt attgttgatc gggctggggt
    30421 tgttggctat caccagcttc tacactctcg cctctcagtt ggcaggtcag gagaaagcca
    30481 ttcatgcgtt gctcagtctg ggcttcggcg gcagtttgat ctccgtgttt gcccgggtcg
    30541 gcggcggaat ctacacgaag gcggcggatg tgggcgcgga tctcgtcggc aaagtcgagg
    30601 cagggattcc ggaggacgac ccacgtaacc cggcggtcat tgccgacaac gtaggcgaca
    30661 atgtcggtga ttgcgcgggt atggcggccg acttgtttga aacctacgca gtcacgaccg
    30721 tggcagcgat ggtgctggcg tttacgatgt tcaaaggtgt caatgcgccg attctgtatc
    30781 cactcgcttt gggtggtgtg accatctttg ccacgatcat cggcattctc tttgtcaaag
    30841 tcagtcccgg tggcgaaatc atgccggcgc tctataaggg cttgtttgtc gccggaggca
    30901 ttgccgcggt cgcatttttc ccgatcacgt cgcagatcat ggagggtgtg ggaggtgtca
    30961 gcggtatcag ttattacctg gcggcattga tggggctggc agtcacgctg gctcttgttt
    31021 tcattacgga ctactacaca tctaaggcgt atgcgcccgt caaagatatt gcgaaggcca
    31081 gtgaaacggg gcatgccacg aacattattg ccgggttggc cgtcggtatg cagtcgacag
    31141 cggcgccggt ggtggtgatt ggagcggcca ttctcgctag ctattggatt tgcggggggg
    31201 cggcgtcagg cgggctttac ggcgttgcgg tggcggccgt atcgatgctg tcgatggccg
    31261 gtatcgtggt tgccattgat gcgttcggac cgatcacgga caacgctggc ggtatcgccg
    31321 aaatggcgca tcttggcaaa gaggtgcgtg acattacgga tcctcttgat gcagtcggca
    31381 atacgacaaa agccgtgaca aaagggtatg cgatcggttc tgccggtctc gcggcagtgg
    31441 tcctctttgc agagtttgcc cgtgaagtgg ctaagggtac gcaagccagc accttcgacc
    31501 tctctaaccc gtccgttctg gtcgggcttt ttctgggagg catgctgccc ttcattttcg
    31561 gcgcgctctg catgaaagcc gtcggtcaag cagccggtct cgtggtggag gaggtacggc
    31621 gtcaattccg gaccatcaaa ggcatcatgg aaggaactgg caaaccggag tacggcactt
    31681 gcgtcgatat cgtcactcag gcggctattg ccaagatgat gattcccggc ttgattccag
    31741 tcgtggctcc gataattgtc ggcgtgattc taggaccgca agcgctgggg ggcgtgctgg
    31801 tcgggagcat cgtcacggga ctgttcgtcg cgatttccat gacgagtgga gggggtgctt
    31861 gggacaatgc caagaagtat atcgaggagc agggattgaa aggcacggat acgcataagg
    31921 ctgcggtgac tggtgatacg gtcggcgatc cctataagga caccgctggc ccggctatca
    31981 atccgatgat caaagtgatc aacattgtgg cgttgctgat cgtctccttg atcgtcaaat
    32041 aggcggatgg tataccggcg tatgtttttc tggcgacgga tgaggaggct cgcgggccgc
    32101 gtgtcgcgga tatgcagcga cgacgacgca cacattcgtg ggtcggcttg acgcgttcaa
    32161 ggaccctaga gtaaggtgac agaagagggt cgttcgaatg cgtccggatg aactggaggt
    32221 gctccgatgg agaaacagga gaggagaccg gaatccagaa aagaatcaca cgccaaagaa
    32281 gaggtgaagc cgaacccgaa ggtagtcgaa tcgggcaaga agatgaaaga ggacatcgat
    32341 aaattggtcg atgaaatcga tgatgtcctg gaaaaaaatg ctgaagagtt tgtgaagaac
    32401 tatgtgcaaa aaggagggga atagtcccct ccttttttat gccaccttcc ggtgcgcgct
    32461 cctcgccttc ctgggccctc cgtccgccca ccagagcgat atcaacacct cgaatcgcca
    32521 tgctatcgtc ctccccatgc cccgtttgac aaagatgggg tgagtcgata ccattcccct
    32581 tatttctcag taatttctga agcgttcagc tctaagcaga ggagtcatgc tcgaaaaact
    32641 ctgggtgttc aggccgtttg atacggtcct acagaacagt ctcgttcgaa cgctgtcgat
    32701 atcgccggtg actgcatcga tcttgctggc ccgcggagtc acgactgccg accaggcgag
    32761 ccgttggctg tccagcccgc aggatgggct ccacgatcca tttttgattc ccgacatgga
    32821 ggctgctgtc gagcgactcc atgtggccct cgcccgtgag gaacgtgtgt gtttctatgg
    32881 ggactacgat gtggacggcg tatcggccac cagcctgtac ctgtcgttct atcgaggatt
    32941 gggagggaat gcctgcggat acattcccca ccgggtacgc gaggggtatg gcctgaacga
    33001 gggggccgtc aggaagctgg cacaggaagg cgtgacgtta ctcgtcactt cagactgcgg
    33061 cacgacgtcg catcatgaaa ttgccgtcgc caaagaactt ggcatggatg tggtggtgac
    33121 agaccaccat caaaccgatg cgacgatgcc cgcagccctt gctgtgctca atccgcatcg
    33181 acgcgacgcc atctatccat ttaaggggct ctgttccgca ggcttggcct ataaggtggt
    33241 cacggcctac cagcgacggt atggctcggg cgaggtcgat ccggaatcct gcctcgactt
    33301 ggtcgcgctg gcgacggtgg ccgatatcgt gccgcttcag gatgagaatc ggattctggt
    33361 gcgcgaggga ctgattcaga tcaatcgtgg tgcccgctgc ggaatccgtg ccttgaaaca
    33421 ggtggccggc atcacaaaag cttgtaccag cgaaacgatc ggatttcgac tggggccacg
    33481 attgaatgct gcgggtcggc tggatcatgc catgctgtgt gtgcaactgc tgactaccga
    33541 atccgatcga gaagcccaac agattgcaga acagctcgaa caactgaatc gacagcgtca
    33601 gcaaatcgag gaagagatta cggcgcaggc gggagcctct ctacagcagg atgaaccggc
    33661 ggctgccatc gtgctgggct cgcgtcactg gcatctgggt gtcgtcggga ttgtcgccgc
    33721 aagattagtg gatcgcttcc atcgcccgag catcgtgatc gctgtggatg agaagggcct
    33781 agggaaaggc tcggccagaa ccgtggaggg atttgatctg tatcaaggat tgtcggcatg
    33841 ccgggatctg ttggaggcct ttggcggcca tccgagcgcg gcgggtttga ctatccaaga
    33901 atcgcgattg gaagagtttc gacggcggtt ttgtgaggtg gcggcacaat gggcgaccgc
    33961 cagtctggtc agaccgacct tgcacgtcga tgcggaggtg aagctcacgg aagtcaattt
    34021 cgacttgatc caggaactcg aatcgttgca tccatttggc gcgggcaatc ccgaaccgac
    34081 attggccgtt cgtggtctcg atgtcgtcga agcccgggtc gtcggtgaga aacatctgaa
    34141 gttgcgcgtc cggcaaggcc gctcgtttat ttttgacagc atcggctttc gcatgggctc
    34201 atttgaagag ctagggttga agacggggag gccggtcgat ctggccttta gccccgagcg
    34261 gaatcattgg aacgggtatg accgcgtcca actgcgcatc aaggcgctgc gcatgagcgg
    34321 cgaggtgtcc taagcatgcc ccacgagaca gtgacagaac tcgatcaatt gatcgagcgg
    34381 gtgaagagct ataacgtcga ggcggacctg gacctcgtac gccgggccta tgatttttcg
    34441 gccaaggccc atgaagggca gatgcgtcga tccggcgagc cgtactttcg ccatcccctc
    34501 gcggtggcgg gcctgttgac ccatctgaag acggacgtga ccgccattgt ggccggcctc
    34561 ctgcacgaca cgctggaaga taccctcgcg actccgctcg aactagagca gcggtttgga
    34621 aaagacgtcg tccatctcgt cgacggcgtc accaagatcg gcaagatcac ctttcggaac
    34681 tacgaggaaa agcaggcgga aaatttccgg aaaatggtgt tgtcgatggc ggacgatatc
    34741 cgcgtcgtcc tcatcaagtt ggcggaccgg ctccataaca tgcggaccct cgagtatctc
    34801 agcgaaggaa aacgacggca gattgcgcag gagacgctgg agatttatgc ccctctcgcc
    34861 aatcgcttgg gaatcgggtg gattaagaac gaacttgaag acctctgttt gaagcacctc
    34921 aagccggatg tctatgaaat gctgcgggtg cgcgtggcca agcgggacga agaccggcag
    34981 cagtacattc atgaagtgat cgacatggtg aagaaggcca tggcggaagc cggcttgtat
    35041 ggggaggtgt ccggtcgccc gaagcatctc tatggcatct accaaaaaat ggaaaagcag
    35101 tcgatctcgt ttgaggaggt ctacgacctc gcagcgttac gcatcatcac cgatatcaaa
    35161 atgaattgtt acgccttgct cggcgtgatc cattcgctgt ggcgcccctt gccaggccgg
    35221 tttaaagatt acatcgccat tccgaaatcc aatatgtatc agtcgttgca taccacggtt
    35281 gcaggaccga agggcgagca tgtggagttt caaatacgca ccgaggagat gcatcgagtt
    35341 tctgagcagg gcatcgccgc gcattggaaa tacaaagagc acggacgtat cgacgagaag
    35401 gacggaaagg tcttcagctg gctacggcag ttcgtggaat ggaatcagga cctgccggac
    35461 aatcgccagt ttatggattc ggtcaagctc gatctcttcc acgacgtagt gtacgtgttt
    35521 accccgcagg gcatggtgaa ggaactgccg aaggggtcca cgccggtcga cttcgcctat
    35581 tccgttcata ctgagatcgg ggaccattgt gtaggggcca aggtcaacgg caagattgtc
    35641 ccgctcaaac acatgatgga aagcggggac atggtggaga ttctcaccgc ggccaatcaa
    35701 acaccccacc gggattggct gaagtttgtc cgcacctccc gcgcgaagac gaagatcaag
    35761 cattggatca aggcagagga acagtctcgc agcatcgaaa tcggcaaacg actgctagag
    35821 gccgaattcc ggcggcatgg actggctccc gctcagatga tgcgctcaga gcaacttctc
    35881 gcggtcgcca agcaggttgg atatgaaact gcggaagaac ttgccgccgc tgtgggtttc
    35941 ggacacgtgc cgacttctca agtcatgagc aaaattgcac cgcccttgcc gggagaagct
    36001 cagaccgtgg cgcctgaggc tccagtgccg cacaagccgg tgatcggtcg cacggatgat
    36061 acgggggtcc aggtcaaggg ggctcgcgac ctcctgatgc aactttcacg ctgctgcaac
    36121 cccgttcccg gcgaccggat tttgggttac atcaccaggg ggcgcgggct cactattcat
    36181 acgattgatt gtccgaacct cgaagcgctg gattacgata aaaaccggct ggtcgaagtc
    36241 gagtgggatc aatcgacgcc tagcacgcat tccgtgaaag tgtcagtgat cgcagtggac
    36301 aagacgggag tgttggcgca tgtctcgaca gccatctcgg agtgccaggc aaatattagt
    36361 cgagcagaaa ttacgacccg tgaagatcgg aaggcgatgc tggacttcat tgttgaagtg
    36421 gcggacacgg cgcacttgtc gagggtgttt aaagcgattg agaaggtgga aggcgtcatc
    36481 accgtgcgcc ggatgcgttc atggcaggag cgggcgtagc ctgaaaggtc tctctgtgcg
    36541 atccttccct cggtgggtga tgtcagcatg actctgggat ctctcgccgg ttccatcctg
    36601 gccattctgg cttgtgcggc tggggtattg ggcgccctga cctacgtgtt tactcgccgc
    36661 ggcaagccgg ttcccgttct gcccttttat gccggtggtg tgggcgtctt tttcgttgtg
    36721 gcatcactga tcggccacag cggtcttggg acttctgttc ctcacttcat tcagtcctgc
    36781 ctgaatgtcg cagcttggct cggcgcagcg ttcttcttct tcacgatcct cgatgtgctc
    36841 atcatcggcg aatggctgat cgagcggggg cagcggtaca tccctgatat cgtccgtcag
    36901 ttgctcatcg gcgcggaagt cgtggcggcc ggtgtgttga ttctctggtt ggtcatgggg
    36961 atcaacctcg tggctctcgt ggcgttgccc acggtagcgg cggcgatggt cggcgtggcg
    37021 ctaaaggata cgttgacgag atttttctcc gggattgaac taggaaaaat cgtcaaggtg
    37081 ggggattggg tcacagtgct tgatcgagaa ggggtggtga ctcatatcgg tatggagcat
    37141 gtcaccttat tgacccgtgc ccatgatgtg gtgtcgttgc caaacgacct ggtcattcaa
    37201 agcggcgtga ccaatttcac cagaccgacg acgacgcatt tttgcaacgt gtttgtcgat
    37261 gccgcgtatc gaaccccgcc tgaacaggtt tgcgcgacgt tgctcgagac tgcgagttca
    37321 gtctcggggg tgttgccgga tcccaaaccc agcgcttttg tgactgcatt taatgaatcg
    37381 gccatccaat accgtatcaa gtttccgatt ggcgatttct cgcaacggga ttccatcgaa
    37441 agtgccatgc gtacctacat ctggcatgct ttcgctcgta cgggcattga gattccgttt
    37501 ccgcagcgcg tggtgcatca atctgctaat ggacaggatg cggctcaaga tcgttacgcc
    37561 attgagcgga tcatggcgaa actgacagcg gtcgattttt tggccacgct tgatacgaag
    37621 caaatcgaag tgctggcgga aggtgcgaga ggggagttgt atttgcctgg cgagcgaatt
    37681 gttcggcaag gagatcctgg agaagtgctg tacgtgattg tctctgggac agcggatgtc
    37741 cgtttagaac agggtggact gactacgacg gtgtcgacac tggaaccagg aaagtttttc
    37801 ggtgagatgt ctttattgac cggtgagccg cggtcggcca cagtggtggc tgtcacgcaa
    37861 ctgtcggtca ttgcggtgag caagtcggct ttgctgcagg tggttcagga ggaccggcgg
    37921 ttgatcgaac gaatcggcga ggtcgtcgcg cgtcgacagg ccgttacggc ggcagcgaag
    37981 gcgcagcttt cgagagatgc ggcggccttg tcagtcgtca cgcatacacg ttctctcgtg
    38041 gaacgtattc agagtttttt ctggggaacg cgtaagtcga catagcgagc ggccggagtc
    38101 cgattagggt tgtttaaagc tgagtttgac gcctcgctga agctgaagcg cggccgcccg
    38161 tcccgctccg agttcaagca cgtagagcgc cccctcctcc gaatgatact gcggacaccc
    38221 gtcgtcttgt ctggtgcaga tgggcgtgtt gcgttcgata tgaacgatgg tttttttcgc
    38281 atccatccag atgatatcga ggggaatttt ggtgtttttc atccagaacg tccaaggttg
    38341 ggcgtcaccg aagatgaaca acatgcctcg atcgtctgcg aggcgctcac gaaacatcaa
    38401 tccctgtgca cgcttcagcg ccgtgtccgc taattctgcc tgaatcacgg taccattcgg
    38461 cattttgacc gcaataagag tttcagtcgg tagatctgcg gcccagccag accaggggct
    38521 cgctactatc gcgaggctcc acaagcaact ggtcagtcgg ctcagccatg tcgagtgagg
    38581 tgtttggctg gaatgatcca tcgattgtgt catggccgca tcctaacggg ctgtttgtga
    38641 gtcgtcaagg cagaggtgat gcgagttctg gagatagaga tgccgccttg caattccgtc
    38701 ggcccctatg ccatcctcgg catatcaatt tgacttcgag ggaggctgtt catgaaagaa
    38761 acgcgacgtg tggtgtacat gcgaggcgtg tttgtctgtc cgaagtgccg ccagtcttat
    38821 gtgcaggaaa agtggatcga agaatttaga atccggtgcc acaagtgcga ctatcgcggg
    38881 acattgaccc aggtgtcagt tgaagagcat atggacgagg aagtaattcg tcgctaggac
    38941 gcggctgttc cgcatggtga atttcttatc tcttgtcttc taatcggaag acatagattc
    39001 tcgccaggcc gttcaaggcc tggccccccc gataccagtc ttgctctcag aatcaccaag
    39061 aggtccatca tggttcagat gtggcgactg ctgcttttgt gcgcgactag tattctgata
    39121 gggcctacta tcggctcatc ggccgatagt ccacccatca cccaagtgct agtggcctat
    39181 cactcactct ccgggaatac cgagcgaatg gcggcagcgg ttgctgaggg ggctcgaacg
    39241 gtgcctcaca cggaggtcat cgttcggcgt ataggaagcg tgacggctga ggagttattt
    39301 gcggcagacg ccatcgttct gggctctccc gtctattggt ccaatatggc tggggaagtt
    39361 aaagcgttta tcgatgactg gcagttcaag ttcggcgtgt ttccggagtt taaattgaag
    39421 aataaggtag gagccgcctt cgcgaccggt ggtcagattt ccagcgggaa agaactcacc
    39481 atgctctcca ttcttgccgc gatgctcgga aattatatga tcactgtcag cggcggcggg
    39541 gcatttggcg cttctgccac aacagaaggc aatagcccag gaattgatga gcaggagctc
    39601 ggcagtgctc gggaattagg ccggcgggta gctgacgttg ccggggcagt caagcgcggg
    39661 tttgcattgc aagaacgtgg cgtgcgctga agcctacaat gaaggacctg tctgggagat
    39721 taggatgaca ccggattgcg gcaggcattc tccaggtgac ttttcgcgag ggcgactaat
    39781 tcgtggatat ttttggcgca acgaccacag catccatttt cccgtagacc gaattcagca
    39841 ataatttgtc tcgtggtcag gtgcccctgg cgacccgctt cccgcacatc tgattccgtt
    39901 aagcccttac ataagcagac atacatgagt ggcgtctccc actgatgatc aattgtctcg
    39961 gccgggcgat gcgaatagct tctcgatact gataaccgtt ctcaactgta aagagtatag
    40021 cggtcgcaat gattcctgtc aacctcaaga cagattgcag acagaacgat gccgtctacc
    40081 caggcgacta tatgaacgag tgacgtaaac aggggctaaa gctgaagctt caggcgttat
    40141 gcggtatgtg gggaaaaggt cttctgctca ttcggaaaat tgcttgaagc gacaacactc
    40201 tagtacggaa acgttcgtgg aagagtcgag ttaaacgatc gtgatattag ttgaggtgag
    40261 gaaagatatg aactgatcat gcaggctgtc gataaacgta caaaggccgg aagttctttc
    40321 gaacttccgg cctttgttgt gctgcggtcc ttcactggcg agagaggttg ccctctcccg
    40381 ccagtgtgag tcttacagcc agttcacgcg ctcggccggc cgaatgtaga tcggctcttc
    40441 gacctga
//
LOCUS       DAOQDS010000039        39993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_320_length_39993_cov_20.357354, whole genome shotgun sequence.
ACCESSION   DAOQDS010000039 DAOQDS010000000
VERSION     DAOQDS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 39993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39993
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_320_length_39993_cov_20.357354"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PK589_11120"
     CDS             complement(<1..959)
                     /locus_tag="PK589_11120"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HNJ20197.1"
                     /translation="MAAPTFAPMEALLVSRLPEGHEWRYEPKWDGFRCLAYKRKTSVT
                     LHSKSGKPLDRYFPDIVRVLAKLKADNFVLDGELLIKGEEGYSFTDLQMRLHPAASRV
                     SMLAKKQPATFVLFDILEAETDSAVMSAPFAARRLRLESFYARYCAKEPALQLSPQTA
                     SIAEAMAWLKSTGWYIDGIVAKHTLAAYEPGERVMQKYKPIRTADCVVGGFRYGSGSE
                     EVGSLLLGLYDKNGRLNHVGFTSGIAKADKPSLTKTLEALVHEPGFDGKAPGGPSRWS
                     TERSTTWTPLSPQIVVEVSFDHVTDDRFRHGTKLLRFRPDKAPR"
     gene            complement(964..1857)
                     /gene="ligD"
                     /locus_tag="PK589_11125"
     CDS             complement(964..1857)
                     /gene="ligD"
                     /locus_tag="PK589_11125"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HNJ20198.1"
                     /translation="MKNHPGEALILHGVTITHPDRVVYKEERICKGDVARYYAAVAPF
                     LLRDITRRPISLLRCPSGIQADCFYQRNVGSGLGGHVHPFAWDYKGQSYQYIYVTDEM
                     GLMQMIQMGVIEIHPWGATIDSIHTPDRMIFDLDPDPTVPFSAVKQAATEIRQRLTKS
                     GLTSLLKCTGGKGLHVVVPLSPTRRWDEVKGWASTLAHAMVEEAPDRFVATMTKSKRA
                     GKIFIDYFRNDYTATSIAAYSLRARPGAPVAVPLQWRDLGTLKSADQFGMTAVLKRIA
                     RQQRPKPLPIQQLPVLKTERF"
     gene            2079..2570
                     /locus_tag="PK589_11130"
     CDS             2079..2570
                     /locus_tag="PK589_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20199.1"
                     /translation="MAKDLDIQDDLPFQYRHWRLQRVGWWAIACILSAGLLGLFGHHP
                     LSRTTVQTQDGTLLVTYDRFGRATSDAEVLITVTPAKQEEGAFRVWFDSEYLDAVRVG
                     SVSPLPLRGEARQGGRAFVIQSDGGPSTVLFSVQFHTFGIIRGHVKINDGKAVPITHL
                     VWP"
     gene            2582..3031
                     /locus_tag="PK589_11135"
     CDS             2582..3031
                     /locus_tag="PK589_11135"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HNJ20200.1"
                     /translation="MDAILRGFIMYLFLLVLFRIAGRRTLGQMTNFDFVLLLIISEAT
                     QNAMIGNDFSVTNGILVILSLVGLDIGFSILKQRFPILNRHLDGLPLVLVDQGRPVQE
                     LMRKTRVDEEDILFSAREKHGLERMEQIKYAVLETNGGISIIPKAAE"
     gene            3137..3364
                     /locus_tag="PK589_11140"
     CDS             3137..3364
                     /locus_tag="PK589_11140"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNJ20201.1"
                     /translation="MGLDYAADDELASRIDQALLATDELNLSRIEVSVEPGVIYLSGI
                     SDDHDSKVQAEQAAQAYAEGRKIVNKIEVDF"
     gene            3514..4608
                     /locus_tag="PK589_11145"
     CDS             3514..4608
                     /locus_tag="PK589_11145"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ20202.1"
                     /translation="MYEDQGGAVELWQYGGFADVSYASTGYHPENHQWRSKETSTRTN
                     ELAPNIGLLYVRKDPAEQSRWGVELAFQAGYDTDDLVPEPKADGTRPVSGADTLRHVA
                     RANVSYLAPIGRGVTFSAGLMKGSKTYEEFYAKYNLNYTRAYITDYNPNFVMGLGASY
                     PITKSLELGVYVINAYRHLAHTNDAPSYLSKMEWRVSDHLTVYENLYYGPDQRATSVD
                     YWRTFSDSTVEWRKPDWRVALSYNVGTEKMADVSGSPRAVWMGAALFTQRHLVGPWSI
                     AVRPECYWDPQGRMTEHEQLLWANTTTLEYKKHLGQQLIIVRLEHRYDRSTGSQGGFF
                     RDGFSAPGVPRLAADQHLTILGVILAFDSA"
     gene            4826..5161
                     /locus_tag="PK589_11150"
     CDS             4826..5161
                     /locus_tag="PK589_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20203.1"
                     /translation="MSMIVLRCPASRLPKVLILLLTWVVVAGLSWADLVGSQAEPTGA
                     LVDAQVATELDLDELRDDGTAISLTEEELPARLSPRTPPFFQIGIPSPDDHSLPTRLL
                     LSKLSVYRL"
     gene            5290..8415
                     /locus_tag="PK589_11155"
     CDS             5290..8415
                     /locus_tag="PK589_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNJ20204.1"
                     /translation="MIDRIVGAALAQRLMVCFAGLALLFGGLYAFHILDVVAYPDPSP
                     PMIEVITQYPGWSGEQMERAITLPIEIALQGMPGLSEIRSLSIFGLSDVKVYFDFGTD
                     YFRDRQEILNRLQLTVLPQNVQPTISPWSAIAEIFRYELTGDHVSLTDLKTTQDWQIR
                     REFKRVPGVIDVSTYGGTTKEYHVELDPGQLMSYNVTLDQVMDGLAKSNTDVGGNYLS
                     MGEQSFNIRGMGLIKKLEDISNTVVTQKEGTPVFVRNLGKTSIGSRVRLGKVGIDDRD
                     DVVEGVVLLQRGAKALPVLDKVHEKVQELNARKLPQGVAIKTFYDRTVLIHTTIETVI
                     DILIAGVVLVSIILYVFLGHFRTAMIVALTVPVALLFTFGMMVLSGQSANLISLGAVD
                     FGIIVDSTLIMVESIFYHLAHKSSAGVTVPMHVMRAAREVGRPIFFATTIIVIAFLPL
                     FTMTGVPGKVFAPMSLTYGFALTGALLMAFTLAPALCSLLLTGPIKERDTAVVEFLSR
                     RYMALLNWGLQREWLVIGIAIVTLVLAMAAVPFIGGEFMPALEEGNIWMRTTFPVDIS
                     FEQAARLVTDIRGIFRQFPEVISAASQLGRPDDGTDPTSFFNAEFLVTLKPFKEWRAD
                     VPTKKALVDQIEQRLAVIPGVTFNFSQAIQDNVQEAMSGVKGENAVKLYGSDLQTLEQ
                     LAAQIELVMKDVHGVKDLGVLHLLGQPNLVIEVNREECARYGLKVGDVNDVVQAAIGG
                     QAVTQIYEGERWFDLVVRFLPEFRRDIESIGNIVVSTPDGARIPLKQLASITEQTGAF
                     IVYRENNERYIPIKFSVRGRDLEGTVREAQERIEQQVPLPAGYRIEWHGEFDQLQEEK
                     ARLAKIVPITLLLILFLVYLVLNNVRDAFLVLAAVPFSLVGGVLALLVTGTHFSISAA
                     VGFISLFGVAVQGALILISRMQELLREGYEIRDAIIKSAEVRMRPVLMTSLAAAIGLL
                     PAAIATGIGAQSQQPLARVVVGGMLTSAALILLVLPVLYQLLHRYRKPSRSTGATASA
                     GTANQGHGSHE"
     gene            complement(8468..9070)
                     /locus_tag="PK589_11160"
     CDS             complement(8468..9070)
                     /locus_tag="PK589_11160"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HNJ20205.1"
                     /translation="MTGTWHRSRPVVWLMASLLAIGLWQVWEGSWMYAKAGLAQLLLQ
                     RAWTRSVEGEAMPKPWPWADTWPVARLRIPRLSVDLIVLAGAHGRTLAFGPGHVGSSA
                     LPGQTGTMMLAGHRDTHFQFLKELQPDDRLEIVGTDGTTRRYRVTERQVMDSQRDSIA
                     TSDERAELILVTCFPFDAITTGGPLRFVVRADAIPQADRS"
     gene            complement(9067..11124)
                     /locus_tag="PK589_11165"
     CDS             complement(9067..11124)
                     /locus_tag="PK589_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HNJ20206.1"
                     /translation="MALATSGVGTPPTDAGSAESPVTLSTGVVPTMGINEVTEGTLLF
                     RTDQAGRFTPAPVLKTDVHIAVTGTIARATVRQEFTNPSTRKGDWLEGIYVFPLPETA
                     AVDHLRMHVGERIIEGQIKERAVAKKVYEQAKQQGQRTSLLDQERPNIFTTSVANIGP
                     GEHITIEIEYQETVRYDDEQFHLRFPMAVGQRYIPGTPVIIEGQAATGSGTMLDTDRV
                     PDASRITPPMQSPRYGAINPLSLSLTLHPGFPIDTMESSFHPIISIPDAEGGYHVTLR
                     ADAVPADRDFQLSWHPVARTEPIATVFTEQKNGQTYAMLMLAPPTQHDEKAPRVPRDL
                     TFIIDTSGSMAGPSIDQAKRALAAALPRLSMQDRFNIIQFNHTVRSLFAVPQPVTTTT
                     IKQAIRYTEHLSADGGTEVLPALRQALKSPQESARLQQIILLTDGQVGNEEELFELLH
                     QRVGTRRLFTIGIGSTPNSHLMRKAAEFGRGTFTYIGKVEEVEQKLERLFRKLERPVL
                     TDVSVDQAGWSGLEQYPATVADLYEGEPIVLAMKADAVPSHATLHGQVGSRPWSLPVS
                     LNQTATQGGLSVYWARQKISSLMDEATKEGRAEVTKTSVIEVALAHHLVSQYTSLVAV
                     DVTPARPTDVPGAEPGQTTNLANMQLSALPKTATGWQLQLLLGLSALVLAALVRRLRN
                     EVA"
     gene            complement(11283..12101)
                     /locus_tag="PK589_11170"
     CDS             complement(11283..12101)
                     /locus_tag="PK589_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ20207.1"
                     /translation="MQKSPIATVSAGPVAPTLVGPDRDMKDVYILSGLVGFLAIVVAA
                     FWYYSQADEAAHSNRSSEALDVAQVSQVLKESTEKVTGGAAMPVTHVETIPLASRSTE
                     ILHDDIQFEVGRKGLTDDGKASLQRHAEFLKNEPDWGVLLQGYTDQQGSMSFNKILGM
                     KRAETVKQQLISLGVPESAIRTVSLGEEGALCIDKSDACRQMNRRVHLEMRKIGQEHM
                     VIPAVITEPVTDSFSLTTDLSMESASAESDSENPTGVGASSNGADSPVESATGN"
     gene            12753..13559
                     /locus_tag="PK589_11175"
     CDS             12753..13559
                     /locus_tag="PK589_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20208.1"
                     /translation="MKRLSSWLLMSWLLMGSLVQAADYQLTLGQGIDVCEACKQNLER
                     MTVHPACEREYSAQFDLESPAWKPFDVAGHLEAMEQVLYFLTAGSESGRNIPDESRFE
                     DTLWRISTKDEPWAYWAQVDINNDGKAEPVLKLHSSLCHARNDGSIGRAYSAPIVVLT
                     ENLSGIDHELTDLVLQNPRQDGRAAGETNYQLYGVFRYKGTTYFDRWDDAGSGDQADQ
                     FTLSIYASTGDTTRKLCRLKELQAKRWFPGLPCTSRQCQTARPLPAHYIP"
     gene            complement(13595..14716)
                     /locus_tag="PK589_11180"
     CDS             complement(13595..14716)
                     /locus_tag="PK589_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ20209.1"
                     /translation="MTEPAQVTWTEEGQSYAAHWRSEWGAPPPKRVVIADDRMTADAA
                     HRLACEGTALLWRGDFQNARQLLLAMARRADRHPPKPGASPTESFHFHRQTQARRARI
                     LGMLLITLEDDYAIRLRRAPDLREACTEAYGPATARALVSLRELLGVIGAHEWRKNGI
                     DIPALGDRIHPHYGVFSPIRSEYVALVADAPLPACTLAFDIGTGTGVLAAILARRGVP
                     RIIATDQDPRALACARENLQRLELSDQVEVRQANLFPEGKAQLVVCNPPWVPARPTSP
                     LEQAIYDPENRMLYGLLNGLAAHLEPGGEGWLLLSDLAEHLALRSRKELLATFEQAGL
                     VVAGKTDIRPTHPRASEQADPLRAARAAELTSLWRLKSR"
     gene            complement(14819..17521)
                     /locus_tag="PK589_11185"
     CDS             complement(14819..17521)
                     /locus_tag="PK589_11185"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ20210.1"
                     /translation="MRIATRLFVRNIWLQAAVMTLAAIVLSDILWTPAPATRQALEWA
                     PYDTWMRLRVQAAPDPNLLLIVRDRASDQQFGTGSWDRALPARLITALHDAGAASIGL
                     DIPLELPSPPNLGGAVSDALLMEAVKSAGTVSYPAIGVAVSDQEAAALSSHFTPGRSA
                     LHPTFDPDRVVRRLPLYHGDGPGELPALGFSLATTFWQVQSNQLERRSGRIHLHNARV
                     AGGSTGDIDIPVDRYGRLLINVAGHPLPTVFPTLSVLDLTRMIDRKENEGLSALVNGK
                     AVLFLTQPTPQPERMMPTGEEAPDMLLQAHLLHTLLTQDWIRDLSPLQRAIVTFALGL
                     STAWGVLRWTDWKGPVLGASMLVLYFFVSWAMLATAHWVLPFIIPVTTSVMILVVGSL
                     LGQMMATRRLVLLEQDMLLVQQNLVAVREALIYRETAVESLEEDLESARASMSHSVSK
                     EAESSRLAAELQLKIAEAQAQEDATRQRMAALEQQLQGMRAATITAEPLGNVEQDALR
                     RECEQAGMITRNPAILTMFRDLKKGARTTVTVLITGEAGTGKELCARAIHRLSPRAAK
                     PFIAVNMAAISPELFESELFGHVRGSFTGAHADRKGYFELAHLGTIFLDEIGDLRLDH
                     QSKLLRVLQDRSFYRVGATTPTTVDVRIVAATNKDLQRGVSEGWFREDLYFRLKGLVL
                     DLPPLRERRDDIPLIAEAYLHEAANHNHQSSMRLSEEAVSTLMQQSWPGNVRELRQCL
                     EQAMALSDSPILSAADLRLSGPASPRTGTSGAAPLLPNPAGDLAVLQQLRQQRFDMQA
                     TAKALGWDRSTVTQRLKGLCFQALVDASGDQAKAAASLAGDPSLLRTVELKLMDYYGH
                     LMETIAPFTNVDDALLDCKRRFKNLPERHFKSVEVLVRRHFSAA"
     gene            complement(17562..18905)
                     /locus_tag="PK589_11190"
     CDS             complement(17562..18905)
                     /locus_tag="PK589_11190"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ20211.1"
                     /translation="MFLQRVSVGRWFLIATVALPLPSLPAGAQEEPSPAAATGTVQAE
                     TPVTETPAVPQADQAPQEAIRVEPLPSEPPAPPPPPPVAPPVQPPLTPLEILARARQA
                     LHIEPDAQEPRLTLGRTLFQLGDTDGAIDEYRTALRFHPTVAQAHLDLGTALMAKQDW
                     RTAMTELQEAVRLDPTLVQAHYSMGTIQYTRGNIQSAIKAYQEALRLKPDFAEAHYRL
                     GLVLKVAGKDKDAAQELEAAALAGLSKAQYFLGNAYRSGQGVEKSQIMAITWWAQAFE
                     QGLPEAAQALTQLRRLAAVKGNLQTKQSKAAAEAFKNYCDQIWLDFPDLDRDQPTETV
                     GTTLLKQGRTAEALPVLLREAYALNEMAHATLVQLYEQGLDGQLPPHGQWILSYLEST
                     AADGAVPSRLALARIYGKGLGLAPDQAKAKSYLKGLPKDDVKRILDDVAAEPPKP"
     gene            complement(19065..19727)
                     /locus_tag="PK589_11195"
     CDS             complement(19065..19727)
                     /locus_tag="PK589_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20212.1"
                     /translation="MMQIPPGPNGSSPRTFFQTLIRTLPLHVLITTSIVGGAALFYSL
                     SVSNRGTWTMLGSWSLTALYILIGLVGGTVAGVLDASRQMVERLEQVLRDWLHTLPAL
                     NRSADTTGRDVSTVRQEYETLVDHSVAQAGRRLRLPQWLERLIRAGVRGLVVDRFIAS
                     CTARGIHVVAPQEFRNWMLAEGVSLGFMPVLDQLAWWRYLLLGVLTLLAAIALTLAFL
                     TT"
     gene            complement(19741..20190)
                     /locus_tag="PK589_11200"
     CDS             complement(19741..20190)
                     /locus_tag="PK589_11200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNJ20213.1"
                     /translation="MSDHACKACVGTWPSQNHFIADCGLTKAYLHDDQFFPGWTVLVF
                     KRHATELFHLSREERSSLIEEVAQVSALLAEEWRAVKINYELLGNQLPHIHWHLIPRL
                     SQDPAPLEPVWRIAHEPVRPEPDALKSLVARLRAIWPAHRHQPPSSA"
     gene            complement(20187..20807)
                     /locus_tag="PK589_11205"
     CDS             complement(20187..20807)
                     /locus_tag="PK589_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20214.1"
                     /translation="MRIPRSMQILAAGACLLLADTAAPVGASDQLVDVTGKVTPFVTL
                     RSRDNFSSEYRYDVTVRNSSPDLFVADSLILVLDHITNTAGQDRENLTGESLLTRMEI
                     IGQDGTTEDGKPYFRIPQGSGPDLTPNSESLPVTVRIRNRDYVAVFTPSFRVMGQRRE
                     PPKQKSQAAAAAAAAAPHKATTDKLIQLLLKKGLITAEEARTLLQP"
     gene            20968..21840
                     /locus_tag="PK589_11210"
     CDS             20968..21840
                     /locus_tag="PK589_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20215.1"
                     /translation="MARTRTTSLPLLVPRAFASTLLRFYDYPDPRRPGRMIKGYDCPH
                     AVRTARMCAAVAQRLGHAPARVKQYQIACLLHDLGRAGLDRTLFGRIWSWARAHGIPT
                     RPREWRAAHPDTRYGKETEAFVARYRTAMETAGITLDPWACEQVEMRLGYARRLAGRL
                     RIVKPELRDLGVAWAPWMGRIMLYYYYPEKLAGAQPWVRQLAEILVACEQFEAFSNQR
                     RGRDYYVRRREDLAEAFAYLNGLQKEGIISRPVVQALRELSAEGVFDAVLEQARGRAL
                     SKRERAFLRQASKE"
     gene            21844..22266
                     /locus_tag="PK589_11215"
     CDS             21844..22266
                     /locus_tag="PK589_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNJ20216.1"
                     /translation="MAVKTVTIRVPMQGDSRMENITAQVQAALEQTPLRAGIVTVFIK
                     HTTASVLIVEDEPGIRADTQVLWERLIPADPRWQHNERNAGEDNGHSHLRGQLQGQSL
                     TIPFTDGAMMLGMWQQIVVLDFDTRARTRELVLQIIGE"
     gene            22294..23394
                     /locus_tag="PK589_11220"
     CDS             22294..23394
                     /locus_tag="PK589_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNJ20217.1"
                     /translation="MSVAWACGVGLLSSVLSGLFSPAGGIAAEATSPSLTAQVAAPTD
                     LSPEEQATIAVFDRATRSVVFIANTAMQRDPWSFNLFEVPQGSGTGFVWSRQGHIVTN
                     YHVIYGADSITVTLADRTEYKAKVIGADPDHDVAVLQIQASEATLQPVTIGNSQSLRV
                     GQKVLAIGNPFGLDHTLTTGVVSALGRTIKSMSNRTIEGVIQTDAAINPGNSGGPLLD
                     SGGRLIGVNTQIVSPSGAFAGIGFAVPVDTINRIVPELIKHGKLIRPGLGISLVPDAM
                     ARRWGVKGVIIGKVGRGSAAERIGLHGARETAGGRIELGDIIVAVDGKPVESIDDLMD
                     SMEQHKVGDQVTIEFARGNRRMQATATLQPVN"
     gene            23482..25233
                     /locus_tag="PK589_11225"
     CDS             23482..25233
                     /locus_tag="PK589_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ20218.1"
                     /translation="MSDHRNADQPGHGGQPSPSRDASTPADPIELIDECLAAFPDGDP
                     RQKLLYKLRHVVTAQSVAQDRRDTELKKLNEVVAKLTAPANRVGLLVEVPAEGVARIV
                     VGGAEYYANIDPRLAAEELKIGAQILVNEAYTVIKALEYDRNGPVLKVAEILPDGRIR
                     FEQDMGRQALILQRSSDLLGVDLKAGDEVRIEPTHRIAIEKFENRQARTHLLDEVPNV
                     TWEQIGGQHQAIDAIRKAIEYPLLHADTFTKYQFTQPKGFLLYGPPGCGKTMIGQAAA
                     ASLSQLVRESHGQAASDGHTKNPPVTSGAFLHIKGPEILNMWLGESERIVRDLFAKAR
                     ARRKEGALPFIFIDEAESVLGTRRSMRSFNINNTLVPMFCAEMDGIESLQDVVIILAS
                     NRPDLIDPAVLRPGRIDRKIKVARPTREAAVEILSVYLTPALPLEPALLEQHDHSHDA
                     ARRAVIEQVVDSLFSRTDQNRVLSIRLRNGQNKVLYRGDLVSGAILSAIVQRAKEKAI
                     ERAVAQTGAAAGDGIRAQDLLDAVQEEYREGEMLPPDDAAEEWLKLLDHHPEQVVGVS
                     SFRRGRPTEERLVNQII"
     gene            25271..26794
                     /locus_tag="PK589_11230"
     CDS             25271..26794
                     /locus_tag="PK589_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HNJ20219.1"
                     /translation="MHLFGIETEYGITREDLDAVDPVEESMELVRAHLTGEFERRWDY
                     HGEDPHEDARGFRVSGLQQDKEEDDFAKVDAHRPYSFHEMKSDLVLPNGARFYNDHTH
                     PEYSTPECRTLKDLLAHDRAGERIVQRAADRRNQRLGGAHVQLYKNNTDFHGHSYGCH
                     DNYLVPRALPFPQLVQGLLPFLVSRQVIAGAGKVGMEAQESGFVPGPFQLSQRADFME
                     AELGVDTMHNRPILNTRDEPHADRAKYRRLHLIVGDANFCEYATALKVGTTRLVLDLL
                     QRGAAPLVELEQPVTAIKQLSRDPDLKTTVRLKDGRTISGLAIQQEYWNAASRVCGGS
                     DPDADWVLREWHDTLQCLGGDRTPLVGKLDWVTKQWLLETFMREEQLAWEDPWLASLD
                     LEYHNLNPDRGLFLGLEAEGKTWRMTTERDIAQALVAGPSDTRGGLRGLCVRRFPEQI
                     TGMQWERVQFAGGLRGRTLEMGDLFDPEAVQACAGLFETAASPADALAAWAKRKDAQ"
     gene            26791..26988
                     /locus_tag="PK589_11235"
     CDS             26791..26988
                     /locus_tag="PK589_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HNJ20220.1"
                     /translation="MMRYTMMPERREAPGDPMPKPTGPSEEGGGPRRPDTGSPDKDNL
                     LKRMRKVDPKQAERYRQRTGQ"
     gene            27044..27817
                     /locus_tag="PK589_11240"
     CDS             27044..27817
                     /locus_tag="PK589_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HNJ20221.1"
                     /translation="MGMQGDFFQLLKEQGYQFGNPSAAAAGLDIPNATTILALKYRDG
                     VLVAGDRRATAGNMVMYDRTDKVLEIDRHSVLAIAGVPATAYEMVRVLEHSFKYYRRT
                     QLQELSFEGKLRAVSKLLKENVPAALAGTGAVVPVFAGFDHEQEAAKIYFYDILGAEF
                     EAVEYAVSGSGSPTIRGILHYVNTWGPQPLAAMPEEQATVQALRLLSSAAEFDSATGG
                     VNRELNLYPVVKLITAAGVQTIPDTHLKHLYERDVVRAL"
     gene            27887..28711
                     /locus_tag="PK589_11245"
     CDS             27887..28711
                     /locus_tag="PK589_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="20S proteasome subunit A/B"
                     /protein_id="HNJ20222.1"
                     /translation="MYEEPYRWIEAVGNRRQYLDEQFAMGAPVVALAYADGIVLLTVS
                     RGTPKLYEIYDRIALGGMGHPADLEKLRFSLLEMAHVEGFNRSPSDVTGARLMKYGLA
                     PAIKQAFEEIYKAPFIAKILLAELGVKPERDLFLTINYDGNFEERRGWAALGATSAVQ
                     VQMGRYIDAQPGFAQASLAQAVETGLCAWAVGSLAQTSSGTSDGDSSVRDAAEKPDAE
                     ALPDKTALLAHVRQTVAEKSLECVLLERNGPGSSKYRTLRPDELGRVLPSAVRASQ"
     gene            28791..30281
                     /locus_tag="PK589_11250"
     CDS             28791..30281
                     /locus_tag="PK589_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HNJ20223.1"
                     /translation="MLNRIFGIETEYGLLVNQDRPDHSPSWVAQRIRDHIFHIARRGV
                     LDLHHRGHDEPPGNGGFLTNAGRIYIDMGHLEYASPECTTLADLVASDRAGDRIIQQA
                     VTALGLDETVSLIKNNIDHETDATFGSHENYLVTRQFPFSRRGLGPLVTFLVTRQVFT
                     GAGRIGCAGDPNEWVQIGGLILHRPGLRDAQDRSIVPFQISQRADYIVNDFFEWVQHN
                     RAIVNTRDEPLADPNQYRRIHLLCGDSNMAEYATALKMGTTGLVLQLIEAGQAPRGLS
                     ISEPVEALQDISRDPDRQWIVRLESGQSISALDIQEQFLAAAQRHCKGQDEETDWVLE
                     QWESVLTDLRHGYEKLVGRIDWASKLWLLETYREAEQLAWDDPMLKSLDLEYHNLHPE
                     RGLCYGLEEEGRGPRLTTDKGVQLAQDHPPRNTRAFGRGELVRHLLTSTATGPGADAA
                     HSFGDHASCEYIINWSNFKLRDTEPFFMADPFKTYVQDVRSHLSAR"
     gene            complement(30289..31209)
                     /locus_tag="PK589_11255"
     CDS             complement(30289..31209)
                     /locus_tag="PK589_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20224.1"
                     /translation="MKTRRFNGRKILGLLLMAWCLQSVADWPPPAYGLEKYGRPLPSM
                     DEPEHEAREEEETLFGGYFLTGAFVKNPTFAARPDNTGLVGMRHMLHLETDLYKQYLT
                     FYTDQNFFSDRTDGWIKLSEWDGTFAFTGVLDRFNWRLQYERDAPLDRRGLKQAYADA
                     LVTARFQAIQDSTWWRRTFPHQNFTAYAGAGWLFHNSQYFARPNNTGRALFRYVAHAD
                     LDVYKNKVVLYGDVNLFTDREASQTLNPSELDWIVGIAVRWQDMELAFYREEDRPLDQ
                     SGLVQKYFAVQLRFAFDVSKGAMERVGWKR"
     gene            complement(31206..31646)
                     /locus_tag="PK589_11260"
     CDS             complement(31206..31646)
                     /locus_tag="PK589_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20225.1"
                     /translation="MLPTGPGSLALSLIMQTAYVRLYIGLMNILLTGFVLGCQTSPPV
                     TEGYPHQQALQAKTLQEVLACAGNPLQKREDGPVTMLRYYREAPLLEESMVSSKGSRP
                     TVHHGCWATVILKDQRVDRVRYHFVPSSVDASNDCEEIFANCQE"
     gene            32266..34998
                     /locus_tag="PK589_11265"
     CDS             32266..34998
                     /locus_tag="PK589_11265"
                     /inference="COORDINATES: protein motif:HMM:NF014343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ20226.1"
                     /translation="MSSDSTARNVTQPLSALCGHEDSLNVPAVERKKTQDAWSLSLAE
                     RRASRRLALRLSTQLFFQGHTWKGVTRSLSLGGLSMDFVSDIPAMLNQRMMLSFSPDE
                     RGLDSVGIVCGIRSSEDVHVPGRTQAAVTLAIQFIHLSDADERVVASLLSHGATAPRH
                     MRLAAALVVQEGEESLIDTGSLQTAATPPASMQLVRGPERPREERRRQQRVVVGLSTE
                     VSLRTRGGDRPLSAGLTTDLSPNGACVHLSAEEDLLGSELELRWLPSAPLQGSSTDSL
                     SATLCSVVGEVVWTRPVAPEARPGCVPVAGRTVLAGVRFLPVAKEAEDIIEHFLGQVS
                     MPSPETKVGSPTVLSEFSECFRPSGLRIVICHDRPQVAPAADAPIIVLAPGYGESKRD
                     YVPLAYYLAGNGFHVVRYDNVNHVGESDGLVTQFRLEDMEADLETVLDHVANQWPGRP
                     IGLVATSLAGRVALKVAGKVSHVRLLTLINGIMDVRHTLQAVHQEDLIGEHLSGVHKG
                     VVNIMGLTIDADRWLAHAVEGEYADLLTTQRDASRLQTPVVLFHAEQDAWVDPASIER
                     VTEAIGPYLRHSFVVPGALHRLQESPRKARTVYRQIAVCCQQELWPARRPERMVEPSH
                     REIGAQNRLERERSKKRKPMGKSDHVAFWQEYLQNFQTIPNVADFWRLMDHLYRLMGD
                     CHKGERILDAGCGNGNFGVFLQLNQAFRQRYGKRGDFRSPDYVGLDFVPAALAQAMSN
                     FEQVGATLQGQFYEGLRAYEPMSMRVCQADLESALPFPDHSFDRVVCNLVLGYVRDPL
                     FTLREYLRVLTPQGRLVISNLKPYADLSAIYRNFVDTAQTKDQVEEGRRLLDNSGRIK
                     EREGEGTFHFHYQTELESLLRAAGVSRPRVYSTFGNQALIAVAEKGMAHVTVAA"
     gene            35032..38040
                     /locus_tag="PK589_11270"
     CDS             35032..38040
                     /locus_tag="PK589_11270"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ20227.1"
                     /translation="MKVLAISALVNALAATGLGMFVYFRDPAAARNRIYGLYCLSIAF
                     WSVFYCGWQLAESKDLALSLLRLVMAGASLIPILYFHHTVTFLDIAVRHAKALKIGYG
                     LAGLFLLVDTTPWLVAGVHPAMSFSFWPVPGPFFHPFLAYFLWYVVYATSLVGVALRD
                     ANGIRRHQYAYMLAASVIGYAGGATNFPLWYGVPLLPYGTVLITMYTALMAYTIVRYR
                     LMNISVVLNKGLGYAIVLAIIVVVTSIGAVLSNRATGHSTPPLLAGTLFLICALWVLS
                     NNPRSSSNTVFSAVCGAACLWLFGCFMLFSATHEEEALFWVRVIYTGVVFLPALTYHF
                     AQRLAGGAVPDRAIGWNYALGGLFWSLLFTTYVVDGQYVYYWGRYPKAGVLHPWLVAY
                     VVGGGGLTVYRLYQGYRFHQTSAPLLGAQLKYTFVALLLGFSASLDFLQNYGIGFYPV
                     GYLLAGLSVTVVAYAIARYELMDISFVPSRPKVMLSIKLAGLIPAYMMILLVIRIFTG
                     TFHYLLAGVLFGLFVIVSSGLANLQKGVERAIGQTLFRERYDAYDTLVQFSKSLVAIL
                     ELKSLTKEIVQTLARVMNIKTASVYVLDKEQGVYSLTASYGFVTRDVIVPPIKMESEF
                     PRALLRTETALVREEIEYDKADVDYLRLLDTLKGLESEVCIPLVSKERLVGFCNLGRR
                     ANHGMYSTEELALLKTLAQHAAIAIDNALLYEDLRRSQLLMRRTDRLRSLETMAGGFA
                     HEIRNPLTSIKTFVQLAPDRRDDVEFMEQFSQVVCEDVERIERLVHEILDYARYMTPK
                     LTQESLNDVVSSCLYFIEVKASSKAITIQKELAPDLPYVKLDRQQIKQVLLNLFINAM
                     EAIGGEQGGRLSVRTRKLVKSVNEPWVQIEVEDSGPGIEPHDLDHIFDPFYTTKHESG
                     EREGTGLGLTIAHQIVQEHGGYVEVFSEVGHGTKFMVSLPVNPPMAEWQAAQPVYDDG
                     RKLAGSFLARPHLGLEQQFSKPDSPLKTRT"
     gene            complement(38142..39125)
                     /locus_tag="PK589_11275"
     CDS             complement(38142..39125)
                     /locus_tag="PK589_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNJ20228.1"
                     /translation="MTILNGFTPALLLRNPHLMTLIPRYWPRLRWKENLPCCERLFTT
                     QPGTQLLGFCHWQDDPTTAPTVLLVHGLEGSADSHYMRGMAAKAFCAGFNIIRMNQRT
                     CGGTDHLTPTLYNSGLSNDYLAIIRELRERDGLSHIWLVGYSMGGNLVLKAAGELQRS
                     EAALAGVVAVSPNIDPTQCVAALEEPRNWLYHAHFLSSLKARMQRKAALFPGQWNLSP
                     LRLMKTITQFDDTYTARDGGYRDATDYYNRAGARHVLSEIAVPTLIITAQDDPFIPAS
                     MFDSPGIKNNRYITLAMLRNGGHCGFFQKRRYGEDRFWAENRIIEWLSSRA"
     gene            39267..39896
                     /locus_tag="PK589_11280"
     CDS             39267..39896
                     /locus_tag="PK589_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNJ20229.1"
                     /translation="MLTAIGDVMRRVYERGWITTRDGNISMRKRDGKYLYITPSGWRK
                     TIVHPEHVVRLEVVTDPTSGHLVPSVREGQQPSGELWMHWNLQRDTKKTRTVVHVHAT
                     HIVAAMYAGIDLQAMSAEFPEISRYTRVGRTVPALPALSRDLADVTAECLGLRKDGTL
                     EFDIVGQKNHGVCAVATDPWAAYEHIERLDHICEIVLKSGVAARSSGKL"
BASE COUNT         7829 a        11085 c        12485 g         8594 t
ORIGIN      
        1 cgaggtgctt tgtccggacg aaaccggagg agttttgtgc catggcggaa gcggtcgtct
       61 gtgacgtggt cgaaactcac ctcgacgacg atttggggcg agagcggcgt ccatgttgtt
      121 gatcgttcgg ttgaccaccg gctgggccca ccgggagcct tcccgtcaaa gcctggctca
      181 tgaacgagcg cttcgagtgt cttggtcagc gaaggcttgt ctgctttcgc aattcccgac
      241 gtaaagccga cgtgattcag gcgcccgttc ttatcataca gtcccagaag aagtgatccg
      301 acctcctctg aacctgagcc ataccggaat cctcccacga cgcagtccgc cgtgcgaatc
      361 ggcttatatt tctgcatgac acgctcgccc ggctcatacg cggccagggt gtgtttggcg
      421 acaatgccat cgatatacca accggtcgat ttgagccagg ccatggcttc agcgatcgac
      481 gctgtctgcg gggataattg gagggccggt tctttcgcgc agtatctcgc gtaaaaagat
      541 tcgagccgaa ggcggcgtgc ggcaaaaggt gctgacatga cggccgagtc tgtttctgct
      601 tccagtatgt cgaaaaggac aaatgtcgcc ggctgttttt ttgccagcat gctcactcgg
      661 ctcgcagcgg gatgcagtcg catctggagg tcggtaaatg aatagccctc ctcgcctttg
      721 atcagcagtt ccccgtcgag gacgaaatta tcggccttca gcttggcaag aacgcgcacg
      781 atgtcgggga aatatctgtc caacggtttg cccgatttcg agtgcagtgt tacggacgtc
      841 ttacgcttat aggcaagaca gcgaaaccca tcccatttcg gctcatatcg ccattcgtga
      901 ccctccggca aacggctgac cagcagggct tccatcggag cgaatgtagg tgcagccacg
      961 cgttcagaac ctttctgtct tcaacaccgg cagctgctgg atcggcaggg gtttcggtcg
     1021 ttgttgccgg gcgatccgtt tcagcactgc cgtcataccg aattgatcag cgctcttgag
     1081 cgtgccgaga tcgcgccact gcaacggcac ggccaccggt gcgccgggtc gggcccgtag
     1141 cgaataggcg gcaatgctcg tggccgtata gtcattacga aagtagtcga tgaatatctt
     1201 ccctgcccgt ttggatttgg tcatggtggc gacaaatctg tctggcgcct cttcgaccat
     1261 ggcatgcgcc aacgtcgagg cccagccttt gacttcgtcc caacgtctgg tgggcgacag
     1321 cggcaccacg acatgcaggc ctttgccgcc cgtgcacttc agcaagctcg tgagtcctga
     1381 ctttgttaag cgctggcgga tttccgtggc cgcttgtttg accgctgaga aaggaacggt
     1441 cggatcggga tcgagatcga aaatcatccg gtctggcgta tggatgctgt cgatcgtcgc
     1501 gccccatggg tggatctcga tcacgcccat ctgaatcatc tgcatgagac ccatttcgtc
     1561 ggtcacatag atgtattggt agctctgccc tttatagtcc cacgcaaatg gatgcacatg
     1621 cccgcctaat ccagagccga cattgcgctg ataaaaacaa tcagcctgga taccgctcgg
     1681 gcatctcaac aaactaatgg gacgccgggt gatgtctcgc aagagaaacg gcgcgacggc
     1741 tgcatagtag cgagcgacat cgcccttgca aatccgttcc tccttgtaca ccacgcgatc
     1801 aggatgcgtg atggtcactc catgcagaat aagggcttcg cctgggtggt tcttcatcgg
     1861 cttggcagtc ccgctgtgcc agtcatcgac cgtggcggtt gttctgcgta tagggaatcg
     1921 agcctgccga gacggcagca tcttgatcta tgaggacatg cgggatgtgg gttttgtcct
     1981 ggcaaaagcc ccagtgactg aagctgcggc ctgtcgccgc tagggattca cctaagtttc
     2041 attcggaaaa ggatggccca tgatcggcac aaaaccttat ggccaaagat ctagacatcc
     2101 aggacgacct cccatttcag taccgtcatt ggagactgca gcgggtggga tggtgggcca
     2161 ttgcctgtat cctttctgcc ggtctcctcg ggttgttcgg gcaccatcct ctttctcgaa
     2221 caacggtgca gacacaagat ggcacgctcc tggtcaccta tgatcgtttc ggacgcgcca
     2281 ccagtgacgc tgaagtcctc attaccgtga cgcccgcaaa gcaggaggag ggagcgtttc
     2341 gggtctggtt cgactcggaa tacctggatg ccgtacgcgt ggggtccgtg tcaccgctgc
     2401 ccctccgagg tgaggcgcgc cagggtggtc gcgcgttcgt catacagtcg gatggcggcc
     2461 cctcgacggt gttgttctct gttcagtttc acacgttcgg gatcattcga ggccacgtca
     2521 aaatcaacga tggtaaggcg gtcccgatca cgcacctggt gtggccgtag agggaggatg
     2581 tatggatgcg attctgcggg gattcataat gtacctgttc ctgctggtgt tgttccggat
     2641 cgcagggcgg cgaacgctgg gccaaatgac caatttcgat ttcgtgctgc tcttgatcat
     2701 cagtgaagcg acccaaaatg ccatgatcgg gaatgatttc tcggtgacca acggtattct
     2761 ggtcatcttg tccctcgtgg gtttggacat tggcttttca atcctgaagc agcgattccc
     2821 gatcttgaat cgtcatctgg atggactgcc gctggtgttg gtggaccagg ggcggcccgt
     2881 gcaggaattg atgcgcaaga ccagggtgga cgaagaggac attctgttct cggcccgcga
     2941 gaaacatggc ctcgagcgca tggaacagat caaatatgcc gtgcttgaaa ccaatggtgg
     3001 aatctcgatc attcccaaag cggcggagta gtgcgtgcta aagaagctgt ctcctaggcc
     3061 gcgatcgtcg cctcatgctg gttccctggc attgcttcca ctgggcctca ttgctttcat
     3121 tggactagct ggctgcgtgg ggcttgatta tgccgccgat gacgagctgg cctcacgaat
     3181 cgatcaggcg ctactggcca cagatgaact gaatctcagc cgcatcgaag tctccgtcga
     3241 accgggggtg atttatctca gcggcatatc cgacgatcac gacagtaagg ttcaggcaga
     3301 acaggcggcg caggcctatg cggaaggcag aaaaatcgtc aataaaattg aggtcgattt
     3361 ttgatgcctc acattgcctc gtcatctcgg gatgacatgc ggttactggc gacgaggact
     3421 catcgtttcc agcacgggta ccgtgacaaa gcctgccctg ccccgcgagc gcataggtgc
     3481 ctggcggttc tcgtcgcgtg ctttctttgt atcatgtacg aagaccaagg cggcgcggtt
     3541 gagctctggc agtatggcgg gtttgccgat gtcagctacg cctctacggg gtatcatccc
     3601 gaaaatcatc agtggcgcag caaggaaaca tccacgagga caaatgagct ggctccgaat
     3661 atcggactgc tgtatgtgcg aaaagatcct gcagagcagt cccgctgggg agttgagttg
     3721 gccttccaag ccggatatga caccgatgat cttgtccctg agccaaaagc cgacgggaca
     3781 cgacctgtgt caggtgcgga taccttgcgt catgtggctc gggcgaatgt cagttacctg
     3841 gcgccgatcg ggcgcggtgt caccttctcg gccggtctga tgaaaggttc caaaacctat
     3901 gaagagtttt atgccaaata caacctcaac tacacgcggg cttacattac cgattacaac
     3961 cctaattttg tgatgggatt gggggcctcc tatcccatca ccaagtcact ggaactcggc
     4021 gtctatgtga tcaatgcgta ccggcacctt gcgcatacca atgatgctcc cagttacctt
     4081 tccaagatgg agtggcgcgt atctgaccat ctcacggtct atgaaaacct ctactacggc
     4141 ccggaccagc gggcgacctc cgtggactat tggcggacct tctccgattc caccgtggag
     4201 tggcgcaaac cggactggcg cgtggccttg tcgtataacg tgggaacaga aaagatggcc
     4261 gatgtctccg gctctccacg agccgtgtgg atgggcgccg ccttattcac gcaacgacac
     4321 ctggttggcc cttggagcat cgctgtccgg cccgagtgtt actgggatcc ccaaggccgg
     4381 atgacggagc atgagcaatt gctatgggct aataccacca ccctcgaata caagaaacac
     4441 ctgggacaac agctcatcat cgtccgtttg gaacaccggt atgatcgatc gaccgggtcg
     4501 caaggcggat tcttccgcga cgggttctct gcgccgggtg tgccgcggct ggcggcagac
     4561 caacatctca cgatcctggg ggtgatactc gccttcgact ccgcctagca gcccgtttta
     4621 cgggggcaga cttcgacaaa tgccaggcac atcgagggat tgctcaccct gtccgccgcc
     4681 tacagcacct attcgctcat cgtcatatgc gtgaacaagc gaggcgcaag gacatgtcgc
     4741 gttgcttgtt gctaggcgcg gcctatggta ctctccagtc attgcgggtc cgagaagggg
     4801 actacgcaac ttgccaatcg ttaacatgag tatgatcgtc ttgcgttgtc cagcctctcg
     4861 tcttccaaaa gtattgatcc tcctgctgac ctgggtggtg gttgccggtc tctcctgggc
     4921 agacctggtt ggttctcaag cagaaccgac cggggcattg gtcgatgcgc aggtcgcgac
     4981 cgaactggat ttggacgagc tgcgggatga tggcacggcc atttccctca ccgaagaaga
     5041 acttcccgcc cgcctttcgc cacggacccc tcccttcttt cagatcggca ttccgtcccc
     5101 ggatgaccat tcgctaccaa cccgattgtt gctctccaaa ctgtccgtct atcgattgta
     5161 acgcgttctc tttcgcttca aggtacggtc cggtgactcc aacttcgtgt gaggacgagg
     5221 cgccggttgt gtgaggcttg cattgggagc ccggtgggca ccggagcgag cagggaagga
     5281 acgggacgta tgatcgatcg aattgtcggg gcggcgttag cacagcgcct gatggtgtgt
     5341 ttcgccggtc tcgcgctgtt gtttgggggc ctctacgcct ttcacattct ggatgtcgtg
     5401 gcctatcccg atccatcgcc gccgatgatc gaggtcatca cgcagtatcc gggctggtcg
     5461 ggcgaacaga tggagcgcgc gattacgctg cccatcgaaa ttgctctcca gggcatgccg
     5521 gggctgtcgg agattcggtc gttgtcgatt ttcggattga gcgacgtcaa agtctatttc
     5581 gacttcggca ccgactactt tcgagaccgg caggaaattc tgaaccgtct gcaactcacc
     5641 gtcctgccgc aaaatgtgca acccaccatt tctccctggt cggccatcgc cgaaatcttt
     5701 cgttatgaat tgaccggcga ccatgtgtcc ctcaccgatc taaagaccac gcaggattgg
     5761 cagattcgcc gcgagtttaa gcgggtgccc ggcgtcatcg atgtgagcac ctatggcgga
     5821 acgaccaagg agtatcacgt cgagttagat cccgggcagc tcatgagtta caacgtgacg
     5881 cttgaccagg tcatggatgg cctggccaag agcaacaccg atgtaggcgg caactacctc
     5941 tcgatggggg agcaaagctt caatattcgc ggcatgggcc tgatcaagaa actcgaggac
     6001 atttccaaca cggtcgtgac gcagaaggaa ggcacgccgg tgttcgtgcg caatctcggg
     6061 aagaccagca tcggttcccg tgtgcgcctg ggcaaagtcg gcatcgatga tcgcgacgat
     6121 gttgtcgaag gcgtcgtgtt gctccagcga ggcgccaagg ccttgccggt gctcgacaag
     6181 gttcacgaga aggtgcagga gctcaacgcc agaaagttgc cgcagggtgt ggcgatcaag
     6241 accttttacg accgcacggt actgatccat accaccattg agaccgtcat cgatattttg
     6301 atcgcgggtg tcgtgctggt ctcgatcatt ctctatgtgt tcctgggaca tttccggacc
     6361 gcgatgatcg tcgcgctgac cgtccccgtg gccctgctct tcacattcgg gatgatggtc
     6421 ctcagcggcc agtcggccaa tttgatctcg ttgggggcgg tcgactttgg gatcatcgtc
     6481 gattcgaccc tcatcatggt cgaaagtatt ttctatcatc ttgcgcataa atcttcggcc
     6541 ggcgtgacgg tgccgatgca tgtcatgcgg gcggcgcggg aggtcgggcg tccgattttc
     6601 ttcgccacga cgatcatcgt catcgcgttt ttgcctctct tcaccatgac aggggtgccg
     6661 ggaaaagtat ttgcgccgat gtccctgacc tatggctttg cgttgaccgg tgcattgctg
     6721 atggcgttca ccctggcgcc ggcattgtgc tcgcttctgc tgactggacc gatcaaagag
     6781 cgtgacacag cggtggtgga attcttgagc cgccgctata tggctttgct gaattggggg
     6841 cttcaacgcg aatggttggt gatcggcatc gcgatcgtga cgttagtcct cgccatggcg
     6901 gccgtgccct tcatcggcgg tgagttcatg ccggccctgg aagaaggtaa tatctggatg
     6961 cgcaccacct tccccgtgga catttcgttc gaacaggcgg ctcgcctggt gacggacatt
     7021 cgcggcatct tcaggcagtt tccggaggtc atcagtgcgg cctcgcaatt aggccgaccc
     7081 gacgacggca ccgatcccac cagtttcttc aatgccgaat tccttgtgac gttgaagccg
     7141 ttcaaggagt ggcgggcgga tgtgccgacc aaaaaagccc tggtcgatca aatcgagcaa
     7201 cgcctcgccg tgattcccgg tgtgaccttc aacttctccc aggcgattca agacaatgtg
     7261 caggaggcga tgtcaggtgt gaagggagag aacgccgtca aactgtacgg cagcgatctc
     7321 cagacgttgg agcaactggc cgcacagatc gagctggtca tgaaggatgt gcatggcgtg
     7381 aaggatctgg gcgtgctcca tctgttgggg caacccaacc tggtcattga ggtcaatcgc
     7441 gaggaatgcg cgcgctacgg cctcaaagtg ggcgacgtca acgatgtggt gcaggcggcc
     7501 attggcggcc aggcggtcac gcagatctat gaaggggaac gctggtttga tctggtggtg
     7561 cggtttctcc ctgagttccg gcgcgatatc gaatcgatcg ggaatatcgt cgtgagtact
     7621 cccgatgggg ccaggattcc actcaagcaa ttggcctcca tcacggaaca gacgggggcg
     7681 ttcatcgtgt accgcgaaaa taacgaacgc tatattccga ttaagttcag cgttcgaggc
     7741 cgcgacctgg aaggcacggt tcgagaggcg caagagcgca tcgagcaaca ggtgccgctc
     7801 cctgccggtt accggatcga atggcatggg gagttcgatc agttgcagga ggaaaaggcg
     7861 cgtctcgcca agatcgtgcc catcactctg ctgctcattc tgtttctcgt ctacctcgtg
     7921 ctgaacaatg tgcgcgatgc tttccttgtc ctcgcggcgg tccccttctc tctggtcggc
     7981 ggtgtgctgg ccttgctggt caccggcacg catttcagta tttcggcggc agtgggattc
     8041 atttcgctct tcggtgtggc ggtgcagggc gccttgattc tgatcagccg gatgcaggaa
     8101 ctgttgcggg aagggtatga gattcgcgac gcgatcataa aaagcgcgga agtccggatg
     8161 cggccggtgt tgatgacctc cctggctgcc gccatcggac tcctgcccgc agccattgcc
     8221 accggtatcg gtgcgcagtc acagcaaccc ttggcaagag tggtggtggg agggatgctg
     8281 acctcagcgg cactcattct gttagtgctg cccgtgctgt accaattgct acaccgctac
     8341 cggaagccat cgcgctcgac cggagcgaca gcctctgcgg gaacagcaaa ccagggccat
     8401 ggttcgcatg aatgaggtat ggggccggcg tgcggcgacc attgtgctca agttcttccg
     8461 gagcgcctca tgagcgatcg gcttgaggaa ttgcgtcggc ccgcaccacg aaccgcaagg
     8521 ggccgccggt cgtgatcgcg tcgaagggaa aacaggtgac caatataagt tccgcgcgtt
     8581 cgtcgctcgt cgcaatgctg tcccgctgcg aatccatgac ctgccgctcg gtgactctgt
     8641 agcggcgggt cgttccatcc gttcccacga tctcgagccg gtcgtccggt tgcagctcct
     8701 tcaaaaattg gaaatgcgtg tctcggtgcc cggcaagcat catcgtgcct gtctgtcccg
     8761 gcagggcact tgacccgacg tgacccggcc cgaaggccaa cgtccgtcca tgggcgccgg
     8821 ccagtacgat cagatccaca gacaggcgtg gtatccgcaa gcgtgcgacg ggccaggtgt
     8881 cggcccaggg ccagggcttc ggcatggcct ctccttccac agaacgggtc caggcccgct
     8941 gcagcagaag ctgcgcgagc ccggccttgg catacatcca tgatccttcc cagacctgcc
     9001 acagtccgat ggcgagaagg cttgccatca accaaaccac cgggcgtgac cggtgccagg
     9061 ttccagtcat gcgacctcgt tccgcagtct gcggaccagc gccgccagca cgagtgctga
     9121 caatcccagc aggagttgca gctgccagcc ggtggcggtc ttcggcaatg cgctcagctg
     9181 catgttggcc aggttcgttg tctgccccgg ctccgctcca ggaacatcgg ttggtcgagc
     9241 cggtgtcaca tcgactgcca ccagactggt gtattggctg acaaggtggt gggccaacgc
     9301 cacttcgatc acggatgttt tggtgacctc ggcacggccc tctttggtgg cctcatccat
     9361 cagggatgag attttttgcc tcgcccaata gacggaaagc cctccttgcg tcgcggtttg
     9421 gttcaacgag accggaagcg accagggacg gctccctacc tgcccatgca aggttgcgtg
     9481 cgacggcacg gcatcggctt tcatggcgag cacaatcggc tcaccttcat acaggtcggc
     9541 gactgttgcc gggtattgtt ccagcccgga ccatccggcc tgatcgacgg agacgtccgt
     9601 cagcaccggg cgttccagtt ttctgaacag gcgctccaac ttctgttcga cctcctccac
     9661 tttaccgata taggtgaagg tgcctcgtcc gaattcggca gctttccgca tcaggtggct
     9721 attgggtgtg gatccgatac cgatcgtgaa cagccgcctg gtgccgacac gttggtgcag
     9781 cagctcgaac aattcctctt cgttcccgac ctggccatcg gtcaataaga tgatctgttg
     9841 cagtctggcg ctctcttgcg ggctcttgag ggcttgccgc agggccggga gcacttcggt
     9901 cccgccatcg gccgagaggt gctccgtata gcggatggcc tgtttgatgg tcgtggtcgt
     9961 caccggctga ggaacggcaa acagcgagcg gaccgtgtgg ttgaactgga tgatgttgaa
    10021 gcgatcctgc atggaaagcc gtggcagcgc tgcggccaag gcacgtttcg cctggtcgat
    10081 cgacgggccg gccatggacc ctgaggtatc gatgatgaac gtaagatcgc gaggaacgcg
    10141 cggcgccttc tcgtcatgct gcgtcggggg ggcgagcatc agcatcgcat aggtctgccc
    10201 attcttctgc tcagtaaaga ccgtcgcgat cggctcggtg cgggccacgg gatgccagct
    10261 gagctgaaag tcgcgatcgg ccggcactgc atcggcccgg agcgtgacat gataaccgcc
    10321 ctcggcatcc gggatgctaa tgatgggatg aaacgatgat tccatcgtat cgatcgggaa
    10381 accaggatga agcgtcaatg aaaggctgag gggattgatc gcgccatagc ggggtgattg
    10441 catcggcggg gtgatacgcg aggcgtcggg gacgcggtct gtatccagca tcgtgccgga
    10501 tcctgtggcc gcctgccctt cgataatgac cggcgtgccg gggatgtagc gctgtcccac
    10561 cgccatggga aagcgtaggt gaaattgttc gtcgtcatac cggacggttt cctggtactc
    10621 gatttcgatg gtgatgtgct cgccgggtcc gatattggct acggaggtcg tgaagatatt
    10681 cggtcgttcc tgatccaaca ggctcgttcg ctgtccctgc tgtttggcct gttcatagac
    10741 cttcttggct acggcccgtt ctttaatctg tccttcgatg atccgctcgc cgacgtgcat
    10801 ccgcaggtga tcgacggcag ctgtttccgg gagagggaag acgtagatgc cctcaagcca
    10861 gtcgcctttt ctggtgctgg gattcgtaaa ttcctgacgg accgtcgcgc gggcgatggt
    10921 gccggtgacg gcaatgtgca cgtcggtctt gagaacggga gccggggtaa atcgaccggc
    10981 ctggtccgtg cggaacagca gggtcccttc cgtgacctca ttgatgccca tggtgggaac
    11041 gactccggta ctcaacgtga ccggcgattc ggccgagccg gcgtccgtgg gaggagttcc
    11101 caccccagag gtggcgagtg ccatgcagac catcaggatg cgtgcacatc tgtgtagtcg
    11161 agcgcggggg agaaagaacc attcattcag catgcgatcc tcatattgtt cgatggaagt
    11221 tgtgcgtggt tgctggtccc tgggaaagac gaggtctttc ccagggagag ctcgacgtca
    11281 cgttagttgc cggttgctga ttcgacagga ctgtctgccc cgttggagga cgcgccgacc
    11341 ccagtagggt tctcgctatc agattcggcg gaagccgatt ccatcgacag gtcggtggtc
    11401 agggagaatg aatccgtgac tggttcggtg attacggcag gaatcaccat gtgctcctgg
    11461 ccgatcttgc gcatttccag atgcacccgt cggttcatct gccggcaggc atcgctcttg
    11521 tcgatgcaca gggcgccttc ttcacccagg ctcacggtgc ggatggccga ttccggcacg
    11581 cccagcgaga tgagctgctg cttgaccgtc tcggcccgct tcatgccaag aatcttgttg
    11641 aagctcatcg aaccctgttg gtccgtataa ccttgaagca acacgcccca atccggttcg
    11701 tttttcagga actctgcatg gcgctggagg gaggccttcc cgtcgtcggt caagcccttg
    11761 cgtccgactt caaactggat gtcgtcgtgc aggatctctg tgctcctgct cgccaggggg
    11821 attgtttcga cgtgagtgac cggcatcgcg gctccccctg tcaccttctc ggtcgactcc
    11881 ttgagaacct gggacacctg ggctacatcg agcgcttcag acgatcgatt gctatgggcc
    11941 gcttcgtccg cctgggagta gtaccagaag gcggcgacga ctatggcgag gaacccgacg
    12001 agaccgctga ggatatagac gtccttcatg tcccgatccg ggccgacgag ggtgggggcg
    12061 actggtcctg cactgacggt agctataggg gatttttgca tggtgcctgc tccttgcatt
    12121 gttgagccgg atcgctccgg cccgttggtt gttggtgaag atttgcccgc tgctgtcttg
    12181 cggggccggc ctgccgatgg tcacctcctt cctgccgaac gcccgccgtt gggcggtgca
    12241 tagagcaagg cggaggccag ccgaatcgac gtcgcggacg atcacgaaac ccttgtgaag
    12301 caaggggtta ggtgaaacgc atcgatgcgt ggccaccgcg attcatgcgg ggactagcgg
    12361 ggatgcgggg ctatccccgc atggagactg cgtggtggat caagcggaat gaggcggaga
    12421 tgcttcggtg atccggatgg acctgactct tgcctctggc gagcgtcgcc tgctaaaagg
    12481 ggccgagccg gttgtgcctt ggtcgaagtt tcggatatcc cgctcgatct gcagcagcaa
    12541 ccagccttcc tcccatcacg tcgtctttgc acccaggttc aactcttgga gagcatgagc
    12601 tctctcgaag gggaatcggg ttgtatggct gtgcttgtga tcggtccggc gccgatccag
    12661 gaatttattt gagagggatg agggctgctg taagatcgac caaaatcccc ggtgatcgcc
    12721 tatgtctcaa ttcaggcagg gagaatcgtt cgatgaagag gttgtcctcg tggttgttga
    12781 tgtcgtggct cctcatgggt tcgctggttc aagcagcgga ctatcaattg accctcggtc
    12841 agggaatcga cgtctgtgaa gcctgcaaac agaatctgga acgaatgacg gtgcatcctg
    12901 cttgcgagcg ggagtacagc gcgcaatttg atctggagtc tccggcatgg aaacccttcg
    12961 atgtggcagg acatctcgaa gccatggaac aggtgttgta tttcctgacc gccggcagcg
    13021 agtcagggag aaacattccg gacgaaagcc gatttgagga tacgctgtgg agaatcagca
    13081 ccaaggacga gccctgggcc tattgggcgc aagtggatat caataatgat gggaaggcag
    13141 aaccggttct caagctccat tcctcactgt gccacgctcg aaacgatgga tcgattggtc
    13201 gggcctattc ggctcccatc gtggtcctca cggaaaatct cagcggcatc gatcacgaat
    13261 tgacggatct ggttcttcaa aatcctcgcc aggatggacg ggctgccggg gaaaccaatt
    13321 accagttgta cggcgtgttc cgctacaagg ggacgaccta ttttgaccgt tgggatgatg
    13381 cgggaagcgg cgatcaggcg gatcaattta cgttgagtat ctacgcatcc accggggata
    13441 cgactcgcaa actctgccgg ttgaaggaat tgcaagcgaa gcgctggttc cccggtttgc
    13501 cttgcacgtc ccgtcaatgc cagaccgcca ggccgctccc cgctcactac atcccgtagc
    13561 gcgatcactc cggcgcgccg ggtcacttcg gcgctcaacg gctcttcaat cgccacaagg
    13621 aggtcaactc ggctgcgcgg gcggcgcgga gaggatccgc ctgttcggag gcgcgtggat
    13681 gagtgggccg gatatcggtc tttcccgcga cgacgagccc tgcctgctcg aaggtggcga
    13741 ggagttcctt cctggatctc agtgccagat gttcggcgag atcggagagt aagagccagc
    13801 cttctcctcc cggttccaag tgggcggcta gcccattcaa aagcccgtac agcatgcggt
    13861 tctcgggatc gtagatcgcc tgctcaaggg gcgaggtggg ccgcgccgga acccatggcg
    13921 ggttgcagac gacgagctgg gctttgccct cggggaacag attggcttgc cgcacctcga
    13981 cctgatcgct tagctccagt cgttgaagat tctcacgggc gcaggcgagg gcgcgaggat
    14041 cctggtcggt cgcaatgatg cgcgggacgc cacggcgggc caagattgcc gccagtaccc
    14101 cggtacccgt gccgatatca aacgccagcg tacaggcagg gagcggggcg tcggccacca
    14161 gcgccacata ctcgctgcgt atcggcgaaa acactccgta gtgcggatgg atgcggtcgc
    14221 ccagggcagg gatgtcgatc ccgttctttc gccattcgtg cgccccgatg actccgagca
    14281 attcccgcag tgagaccaat gcacgagccg tcgcggggcc gtaggcttct gtacaggctt
    14341 cacggagatc gggcgcacga cggaggcgga ttgcgtaatc atcttccaga gtgatcagaa
    14401 gcatgcccag gatgcgggcc cggcgtgctt gcgtctgccg gtgaaagtga aaggactccg
    14461 tgggagaggc gccgggtttc ggaggatggc ggtctgcgcg tctggccatg gcaaggagca
    14521 attgtcgcgc attctggaag tcgcctcgcc agagcaatgc ggtgccctcg caggccaagc
    14581 gatgggccgc atcggcagtc atgcggtcgt cggcgatcac cacacgtttc gggggaggcg
    14641 cgccccattc cgagcgccaa tgcgcggcgt atgactgtcc ttcttctgtc cacgttacct
    14701 gtgccggttc agtcatgcct ggctccgcgc gctattgacg gaaatacccc ctgctgacgc
    14761 ctgtgtgtct gcgccgacgc accgaacggt ctcattgtga cagagtgagg cgttcggatc
    14821 atgcggcaga aaaatgtcgc cgcaccaaga cctcgacgga tttgaagtgg cgctcgggca
    14881 ggtttttgaa acgccgcttg cagtcgagca gggcgtcgtc cacgtttgtg aacggagcga
    14941 tggtctccat gaggtggcca tagtagtcca tcaatttcag ctcgactgtc cgcaggagag
    15001 acgggtcgcc cgccaatgag gctgcggctt tggcctggtc tccgctggca tccacgagcg
    15061 cttgaaagca gagccctttg agtcgttggg tcaccgtgct gcgatcccat ccgagagcct
    15121 ttgccgtcgc ttgcatgtcg aagcgctgtt gacgcaattg ttgtaagacg gcgaggtcgc
    15181 ccgcaggatt gggcaacagc ggcgcggcac cggatgtgcc ggttctcggt gacgctggcc
    15241 cggacaggcg gagatccgcc gcggagagaa tcggactgtc cgagagcgcc atggcctgct
    15301 ccaggcactg ccgcaattcc cgcacgttgc ccggccagga ttgttgcatg agcgtcgaga
    15361 ctgcctcttc cgaaaggcgc atcgatgatt ggtggttgtg gttcgcagct tcgtggagat
    15421 aggcctctgc aatgaggggg atatcgtccc gacgctcccg caggggtggc aggtccagca
    15481 cgaggccttt gagtcggaaa tacaagtctt cacgaaacca gccttccgaa acgccacgct
    15541 ggagatcctt gttcgtcgcc gcgacaatgc gaacgtccac tgtggtgggg gttgtggcgc
    15601 cgactcgata gaaggagcga tcctggagca cgcgcagcag tttgctttga tggtccagcc
    15661 gcaggtcgcc gatttcatcg agaaaaatcg tcccgagatg cgccagttcg aaatagcctt
    15721 tccggtccgc gtgcgcgccg gtaaaactgc cgcggacatg cccgaagagt tcactctcaa
    15781 acagttccgg tgagatggcc gccatgttga cggcaatgaa tggcttcgcc gctcgtggac
    15841 tcaaacggtg gatcgcgcgg gcgcagagtt ctttgccggt tcccgcctcg cctgtgatca
    15901 ggacggtcac ggtcgtgcgt gcgcctttct tcaggtcgcg aaacatcgtg aggatcgccg
    15961 gattcctggt gatcattcct gcctgctcac attcccggcg aagggcgtcc tgttccacgt
    16021 tgcccagcgg ctctgccgtg atcgtcgcag cccgcatgcc ctgcagctgt tgttcaagcg
    16081 ccgccatccg ttgccgcgtc gcatcttcct gggcctgggc ctcggcaatc ttcagttgga
    16141 gctccgccgc gagcctgctc gattcggctt ccttggagac tgaatgagac atgctggcgc
    16201 gagcggattc taaatcctct tccagacttt ccacggccgt ttcccggtag atcagggctt
    16261 cccggacggc cacgaggttc tgttgaacca gcagcatgtc ctgttccaac agcaccaggc
    16321 gtctggtcgc catcatctgg cccaggaggc tgccgacgac gagaatcatg acgctggtgg
    16381 tgacaggaat gatgaacggc aagacccaat gggcggtcgc cagcattgcc caactgacga
    16441 agaaatagag caccagcatg ctcgcgccca gaaccgggcc tttccaatcc gtccagcgca
    16501 ggactcccca ggcggtggag aggccaagtg cgaatgtgac gatggcccgt tggagcgggg
    16561 aaaggtcccg aatccagtct tgcgtcagca gcgtgtgcag cagatgggct tgcagcagca
    16621 tatccggtgc ctcttcgccg gtcggcatca tgcgttccgg ttgaggagtc ggttgagtga
    16681 ggaacagcac ggctttgccg ttgaccaggg cgctgaggcc ctcattctcc ttccggtcga
    16741 tcatgcgggt gagatccaac acagagaggg tagggaagac cgtgggaagc ggatgtccgg
    16801 caacgttgat gagtagccgg ccgtagcggt caaccgggat gtcgatatcg cctgtcgatc
    16861 cacccgcaac tcgcgcgttg tgcagatgta ttcgccccga tcgtctttcg agttgattcg
    16921 attggacttg ccagaaggtc gtggccaatg agaatcccaa ggccggaagt tcaccggggc
    16981 cgtcgccatg atacagcggc aaccggcgga ccacgcgatc ggggtcaaac gtcgggtgca
    17041 gggcgcttct tcccggtgtg aagtgggagg acagagccgc cgcctcctga tctgagacag
    17101 ccaccccgat cgctgggtac gagacggtgc cggcagattt caccgcttcc atcagcaggg
    17161 cgtcactgac ggcgcctccg agattgggcg ggctgggaag ctcgagagga atatccaggc
    17221 cgatcgaggc ggcgccggca tcatgcaggg cggtaatgag tcttgcgggg agcgcccgat
    17281 cccacgaacc ggtgccgaac tgttgatcgc tggctcggtc tctgacgatc aggagcaggt
    17341 tcgggtcggg cgccgcttgg acacgaaggc gcatccaggt gtcatagggt gcccattcca
    17401 acgcctggcg cgtggccggg gccggcgtcc ataggatgtc gctgaggacg atcgcggcca
    17461 gcgtcatcac cgcggcttgc agccagatat tcctgacgaa caggcgggtg gcgattcgca
    17521 tggggtgtca cggacgaacc gatactgaaa ttgtctgctc gttacggctt gggaggttca
    17581 gcggcgacgt cgtcgaggat acgtttgacg tcatccttcg gcaaaccctt cagatagctc
    17641 ttggccttgg cctgatcggg agccagaccg agcccttttc cataaatacg ggccagtgcc
    17701 aggcgggaag gaacggctcc atcggcggca gtggattcca ggtaactcaa aatccattgg
    17761 ccatgcggcg ggagttggcc gtcgagtccc tgttcgtaca gttgcaccag cgtggcatgg
    17821 gccatctcgt tgagggcata ggcttcgcgc agcaggaccg ggagggcttc ggcggtgcgg
    17881 ccctgcttca gcaacgtcgt gccgacggtc tccgtgggtt ggtcgcggtc gaggtccggg
    17941 aagtccagcc agatttgatc gcaatagttc ttgaacgcct cggcggccgc tttggactgt
    18001 ttggtctgca ggttgccttt gaccgctgcc agtcgccgga gttgtgtcag tgcctgcgct
    18061 gcttccggta gcccctgttc gaacgcctgt gcccaccagg tgatggccat gatctggctt
    18121 ttctcgacac cctgtcccga gcgataggca ttgccgagaa agtattgggc tttggacagc
    18181 ccggccagcg cagccgcttc aagttcctgc gccgcgtctt tgtctttccc cgcaactttg
    18241 agtacgaggc ccaggcgata atgggcttcg gcaaaatcag gcttcaaccg cagcgcttcc
    18301 tggtaggcct tgatggccga ctggatgttg ccgcgtgtgt attggatcgt gcccatgctg
    18361 tagtgcgcct gaacgagcgt cggatcaagg cggacggcct cctgcaattc cgtcatggcg
    18421 gttcgccagt cctgcttggc catgagcgcg gtgccgagat cgagatgcgc ctgcgcgacc
    18481 gtgggatgga agcgcagcgc ggtccggtat tcatcgatcg cgccatccgt atcacccagt
    18541 tggaagagcg ttctcccgag ggtgagccgg ggctcctgag catccggttc gatatgcagc
    18601 gcttgccggg ccctggccag aatttcgagt ggtgtcagag gaggctgaac cggcggcgcg
    18661 accggtggag gtggcggtgg cgcgggaggt tcgctgggaa gcggttccac gcggatcgct
    18721 tcttgaggag cctggtcagc ctgcggaaca gcgggtgttt cggtgacggg tgtttctgct
    18781 tggacggttc ctgttgccgc ggcgggagaa ggctcttctt gggcaccggc cggtagagac
    18841 ggaagaggca atgccactgt cgctatgaga aaccaccgtc cgacggagac gcgttgcaag
    18901 aacataggtg ctggcagggc tccacgcaaa aggtatccga ctatatacac atagatgacg
    18961 ggaaatttcc atgggatctc gcggggaacc gtgcccatga cccgccggga ccggatcggg
    19021 gtgaaaccag tccgatgtac gaagtggatc aatcggaacc acgctcacgt cgtcaggaag
    19081 gccagcgtca gcgcaatggc agccaggagc gtgagcactc ctaagaggag gtagcgccac
    19141 caagccaatt gatccaggac cggcatgaat cccaggctca cgccttcggc gagcatccag
    19201 tttcgaaact cctgaggcgc gacgacatgg attccccgtg cggtacagga cgcgatgaag
    19261 cgatcgacca ccagaccccg cactcccgcg cgaatcaacc gttcaagcca ctgcggtagc
    19321 cgcagccgcc gcccggcttg cgcgaccgag tggtcgacga gggtttcgta ttcctgccga
    19381 accgtggaca cgtctcggcc ggtggtgtct gcgctccggt tcagggcagg caacgtatgg
    19441 agccagtcac ggaggacttg ttccaggcgt tccaccatct gccgggatgc gtccaatacg
    19501 ccggccacag tcccgcccac gagcccgatg aggatataca gagccgtcag ggaccagctt
    19561 ccgagcatgg tccaggttcc acggttcgag acggagagac tgtaaaagag tgccgcccct
    19621 cccacgatgc tggtcgtgat cagtacgtgc agaggcagag tgcggatcaa cgtctggaag
    19681 aaggtgcggg gactacttcc gttcggccct ggcggaatct gcatcatgac cggaggtcgt
    19741 ttaggcactg gatggcggct gatgccggtg agccggccag attgcccgga ggcgggccac
    19801 cagggacttg agggcatccg gttcgggccg gactggttca tgcgcgatgc gccacaccgg
    19861 ctcgagcgga gccgggtcct gcgacaggcg gggaatcagg tgccaatgga tgtgcggaag
    19921 ctggttgccg agcagctcgt agttgatctt caccgcccgc cattcttcgg caaggagggc
    19981 ggatacctga gcgacttctt cgatgaggct gctgcgttcc tctcgtgaga gatggaacag
    20041 ttcggtcgcg tggcgcttga agacgagcac agtccagccg ggaaagaatt ggtcgtcgtg
    20101 gagataggcc ttcgtgaggc cgcaatcggc gatgaaatga ttctggctcg gccaggtccc
    20161 gacacaagcc ttgcaagcat gatcgctcat ggttggagaa gggtccgtgc ctcttccgcc
    20221 gtgatcaacc cctttttgag caggagctga atgagtttgt ccgtggtcgc tttatgcggt
    20281 gcggcggcgg ccgcggcagc tgctgcttga gatttctgtt tcggcggctc gcgtcgctgg
    20341 cccatgacac ggaatgaagg ggtgaacacg gctacgtagt cccggttccg gatacggacg
    20401 gtgacgggga ggctttcact gttcggggtg agatcagggc ccgatccttg cggaatgcgg
    20461 aaatagggtt ttccatcctc tgtggtcccg tcctgcccga tgatttccat cctcgtcaaa
    20521 agggattcgc ccgtcaggtt ctcacgatcc tgtccggcgg tattggtgat atggtccagg
    20581 accaggatga gactgtcagc cacaaagaga tcgggtgaac tgttgcgcac cgtcacgtcg
    20641 tagcgatatt cgctggaaaa attgtcgcgg ctgcgcagcg tgacgaacgg cgtgaccttg
    20701 ccggtcacat ccaccagttg atcggacgca cccacgggag cggcggtgtc cgccagcaga
    20761 aggcacgcac cggctgccag gatctgcatg gaccgaggta ttctcatggt tccctcccgt
    20821 ggtcgatcga catccgattc agaataacaa agccccgcag ccagtgcgag gaaatcctgt
    20881 cctgcgcgac ggtctgcttg acacgttctt ttcatggacg atattctgcc gggcggtcaa
    20941 tcgtcgcccc cggtgcatca ctcctgcatg gcacgtacac gtactacctc ccttcccttg
    21001 ctggtgccgc gggcattcgc ctctacgctg ctacgatttt acgactatcc cgatccgcgt
    21061 cgtcccgggc gaatgatcaa ggggtatgac tgccctcatg ctgtacggac ggcccggatg
    21121 tgcgcggcgg tggcccagcg gttgggccac gcaccggcgc gggtgaagca gtatcagatc
    21181 gcctgtctcc ttcatgactt aggccgtgcc ggactcgatc ggacactctt cggccgcatc
    21241 tggtcctggg cccgcgccca tgggattcct accaggccgc gcgaatggag ggcggcccat
    21301 cctgacacac gctacggcaa ggagaccgaa gcctttgtcg cccgctatcg aacggcgatg
    21361 gagacagccg gcatcaccct cgatccatgg gcttgcgaac aggtggagat gcgtctgggg
    21421 tatgcacgcc gtctggccgg gagactccgt atcgtaaaac cggaattgcg tgacctggga
    21481 gtggcctggg cgccgtggat ggggcggatc atgctctatt attactaccc tgaaaagctc
    21541 gccggtgcgc agccttgggt tcggcagctg gccgagatcc tcgtcgcgtg cgaacagttc
    21601 gaagcgttca gcaatcagcg ccgtggtcgt gactattatg tgcgtcgccg agaagatctg
    21661 gccgaagcgt tcgcctacct gaacggtctg cagaaagaag gcatcatcag tcgtccggtt
    21721 gttcaggcct tgcgagagtt gtcggcggaa ggcgtgtttg atgctgtgct cgaacaggcg
    21781 aggggccggg ctttatcaaa gagagaacgg gcatttttac gccaggctag taaggagtag
    21841 cggatggccg tcaaaacggt aacgattcga gtgcccatgc agggcgacag ccggatggaa
    21901 aatatcacag cccaggtcca ggcggcgctt gagcaaacgc cactgcgggc cggcatcgtg
    21961 acggtattca tcaagcatac gaccgcctcc gtgctgatcg tcgaagatga gcccggcatt
    22021 cgtgccgata cccaggttct ctgggagcgc cttattcccg ccgatcctcg ctggcagcac
    22081 aatgagcgca atgcgggcga ggataatggg catagccatc tgcgcggaca gctgcagggc
    22141 cagtccctca cgattccctt cacagacgga gccatgatgt tgggcatgtg gcaacagatc
    22201 gtggtgctgg attttgatac gcgcgcacga accagagagt tggtgcttca gatcatcggt
    22261 gaatagatgc gagcggtgaa agtgcgcaca cacatgtctg tcgcatgggc ctgtggagtg
    22321 gggctgttgt cgagcgtgct atcggggctg ttttccccgg ccggtggtat cgcagccgaa
    22381 gcaacatccc cttcgctcac ggcgcaggtt gcggccccga ctgatctgag tccggaggaa
    22441 caggcgacga tcgccgtgtt cgatcgggct accagatcgg tggtgtttat cgccaatacg
    22501 gcaatgcagc gtgatccctg gtcattcaat ctgttcgagg tgccgcaagg gtccgggacc
    22561 ggatttgtct ggagccggca agggcacatc gtgaccaact accacgtgat ttacggggcg
    22621 gattccatca cggtgacact ggcggaccgg accgaatata aggcgaaggt catcggtgcc
    22681 gatccggatc atgatgtcgc ggtgctgcag atccaggcgt ccgaggctac actccagccg
    22741 gtcaccatcg gcaactctca gtctctgcgg gtgggccaaa aggtgctggc gatcgggaat
    22801 ccctttggcc tcgatcacac cttgacgacc ggtgtggtca gcgcgttggg ccgcaccatc
    22861 aaatcaatga gcaaccggac cattgagggc gtgattcaga ccgatgccgc gatcaacccc
    22921 ggcaactccg gtgggccctt gctggacagc ggcgggcgct tgattggtgt gaacacgcag
    22981 atcgtgagcc ccagcggagc gtttgccggg atcgggtttg cagtgccggt cgatacgatc
    23041 aaccgcatcg ttccggaatt gatcaagcat ggaaagttga tcaggccggg attgggcatt
    23101 tcgctggtgc ctgatgccat ggcgcggcgg tggggtgtca aaggcgtcat catcggcaag
    23161 gtcgggcgcg gcagtgcggc ggagcgcatc ggacttcatg gggcgcggga aaccgccggc
    23221 ggccgcattg aattaggcga tattattgtg gccgtggatg gcaaaccggt ggaaagcatc
    23281 gatgacctga tggattccat ggaacaacat aaggtcggtg atcaggtcac gatcgaattc
    23341 gcgcgcggca atcgccggat gcaggcgacg gcgactctcc aaccagtcaa ttgagtggag
    23401 tcagagccac cagaacgccg ctggcggccc ttctcgacaa cctgttagga tgaccggagt
    23461 gagattatcc ggaggaatga tatgagtgac catcggaatg cggatcaacc tggccatggg
    23521 ggacagccat cgccatcgcg cgatgcctcc acgcctgccg atcccatcga actgatagat
    23581 gaatgtctgg cggcctttcc cgacggcgac cctcgtcaga agctgcttta taaattgcgc
    23641 catgtcgtga cggcgcagtc ggtggcgcag gatcggcggg ataccgaatt gaagaagctc
    23701 aatgaggtcg tcgcgaagct cacggcgccg gccaaccgtg tgggcctgct cgtggaggtt
    23761 ccggctgagg gcgtggcgcg tatcgtggtt ggcggggcgg agtattatgc caatatcgac
    23821 ccgcggctgg cggcagagga gctcaagatc ggagctcaga ttctggtcaa cgaggcgtat
    23881 acggtgatca aggccctgga gtatgaccgc aatgggcccg tgttgaaggt tgcggagatc
    23941 ttgcctgacg gacgtatccg gtttgagcag gatatgggac ggcaggccct gattcttcag
    24001 cggtccagcg acttgctggg cgtggacctc aaggccggtg atgaagtccg catcgaaccc
    24061 acacaccgga ttgccattga aaagtttgaa aatcggcagg cgcggaccca cctgcttgac
    24121 gaagtgccga atgtcacgtg ggagcagatc ggcgggcagc atcaggccat tgatgccatc
    24181 cgtaaggcga tcgagtatcc gctgctccat gccgacacct tcacgaagta ccaatttacg
    24241 cagccgaaag ggtttctgct gtacggtccc cccggttgcg gcaagaccat gatcgggcag
    24301 gcggcggcgg ccagtttgtc gcagctggtg cgcgagtcgc acgggcaggc tgcttcagac
    24361 ggccacacga aaaatccgcc ggtgacgagc ggcgcgttcc tccatatcaa gggaccggaa
    24421 attctcaaca tgtggttggg cgagtccgag cgcatcgttc gggatctctt cgccaaggcg
    24481 cgggccagac gcaaggaagg ggcgctgccg tttattttta tcgacgaagc ggagtcggtg
    24541 ttggggacca gacggtcgat gcggtcattc aacatcaaca atacgctggt gccgatgttc
    24601 tgcgcggaga tggacgggat cgagtcgttg caggatgtgg tgatcattct ggcctccaac
    24661 cggccggatt tgatcgatcc cgcggtgctg cggccgggcc ggatcgatcg caagatcaaa
    24721 gtggcgcgtc cgactcgaga ggccgctgtg gagattttgt ccgtgtatct cacacccgcg
    24781 cttccgctcg agcccgcgtt gctggagcaa cacgatcact ctcacgacgc ggcgcgccgc
    24841 gcggtgatcg agcaagtggt cgacagtctc ttctcgcgaa ccgatcagaa ccgtgtcctg
    24901 tccatacgtc tgcgcaacgg gcagaacaag gtgctgtatc gcggggatct tgtcagcggg
    24961 gccattctgt ctgcaatcgt ccagcgggcg aaggaaaagg cgatcgagcg ggcagtggcg
    25021 cagaccggcg cggcagcggg cgacggcatt cgggcgcagg atctccttga tgccgtgcag
    25081 gaggaatatc gagaagggga aatgctgccc cccgacgatg cggcggaaga atggttgaag
    25141 ctcctggacc atcatccaga gcaggtggtg ggcgtgtctt cgttccgccg gggcaggccg
    25201 acggaagaac gtttggtcaa tcagattatt tgatcggcgg gaacgcggtt cttctcacgc
    25261 gtaacggact atgcatctct tcggcattga aacagagtac ggcatcacca gggaggatct
    25321 cgacgcggtc gatccggtcg aggaatccat ggagctggtg cgggcccatc tgaccggcga
    25381 gttcgaacgg cggtgggatt accatggcga agatccgcat gaggatgcgc gcggcttccg
    25441 tgtctcgggt ctgcagcagg acaaggaaga agacgacttc gccaaggtcg atgcccatcg
    25501 tccgtattcg ttccatgaga tgaagagcga tttggtcttg ccgaacggcg cgcggtttta
    25561 caacgaccac acgcacccgg agtattcgac ccctgaatgc cggacgctca aagatctcct
    25621 ggcgcatgac cgggcggggg agcgcattgt gcaacgcgcg gcagatcgtc gcaatcagcg
    25681 gttgggcggg gcgcatgttc agctctacaa aaataatacc gactttcacg gtcacagtta
    25741 tggctgccac gataactacc tcgttccacg ggcactgcct ttcccgcaat tggtgcaggg
    25801 gctcttgccg ttcctggtca gccgccaagt gatcgccggc gcagggaagg tgggtatgga
    25861 agcccaggag tccggctttg tgccgggccc ctttcaactc tcgcaacggg cggattttat
    25921 ggaagcggag ctgggtgtgg acacgatgca caaccggccc attctcaata cgcgcgacga
    25981 gccccatgcg gaccgggcaa agtatcggcg gctgcatttg attgtcggtg acgccaactt
    26041 ctgcgagtac gccacggcgc tcaaagtcgg gaccaccagg ctggtgctcg atctgctcca
    26101 gcggggcgca gctccgctgg tagagttgga gcagccggtg accgccatca agcaattgtc
    26161 tcgcgatccc gatctcaaaa cgaccgtgcg gttgaaggat ggacggacga tctccggcct
    26221 ggctattcag caagagtact ggaacgcagc aagccgggtt tgcggaggga gcgatcccga
    26281 tgccgattgg gttctccgtg aatggcatga cactctgcag tgcctcgggg gggatcgcac
    26341 accacttgtc ggcaagctgg attgggtcac gaaacagtgg ttgctggaaa ccttcatgcg
    26401 cgaggagcag ctcgcgtggg aggatccctg gctcgccagc ctggatttgg aataccataa
    26461 tctcaatcct gaccgtggct tgtttctcgg cctcgaagcc gaagggaaga cctggcggat
    26521 gacgaccgag cgcgacattg cccaggcgct cgtggctggt ccctcagata cccgtggagg
    26581 attgcgcggc ctgtgtgtca gacggtttcc cgaacaaatc acggggatgc agtgggagcg
    26641 ggtgcagttc gcgggcgggt tgcgcggtcg tacgctggag atgggcgatc tgttcgatcc
    26701 ggaggcggtg caggcttgtg ccgggttatt cgaaaccgcg gcgtctcctg ctgatgcctt
    26761 ggctgcgtgg gcaaaacgaa aggatgcaca atgatgcgat atacaatgat gccggagcgc
    26821 cgtgaagccc ccggcgatcc gatgccgaaa ccaacaggcc cttcggaaga aggcggcggt
    26881 cctcggcggc cggataccgg ttcgccggac aaggataatc tgctgaagcg gatgcgaaaa
    26941 gtcgacccca agcaggcaga gcgctatcgg cagcgcacgg gacaataaac gtgatagtca
    27001 tcgtgaaacg taacggttgc gggtgtgtct aacgaggtag gcaatgggga tgcaagggga
    27061 cttttttcag ctcttgaaag agcagggtta tcagttcggg aatccgtccg cggctgcggc
    27121 gggattggat attccgaacg ccaccacgat tcttgcgctc aagtatcgtg acggagtgct
    27181 ggtggcgggt gaccgccggg ccaccgccgg caacatggtg atgtatgacc ggaccgacaa
    27241 ggtattggag atcgatcgcc atagtgtcct ggcgattgcc ggtgtgccgg ccactgccta
    27301 cgagatggtt cgtgtgctgg agcattcatt caagtattac cgtcgcacac agctacagga
    27361 gctcagtttt gagggtaaac ttcgcgccgt ctccaagtta ctgaaagaaa acgtgccggc
    27421 ggcgttggct ggaaccggag cggtggtgcc cgtttttgcc gggtttgatc atgagcagga
    27481 agcggccaag atctacttct acgacattct cggggctgag ttcgaagcgg tggagtacgc
    27541 cgtgtccggc tccggctcgc cgacgatccg gggcatcctt cattatgtga acacctgggg
    27601 ccctcaaccg ctcgcagcca tgcctgaaga gcaggcgacg gtgcaagccc tgcgattgct
    27661 ctccagcgcg gcggagttcg attcggcgac cggtggcgtg aatcgtgagt tgaacctgta
    27721 tccggtggtg aagcttatta ccgcggcagg ggtgcagacc attccggaca ctcatttgaa
    27781 acacttgtac gagcgtgacg tcgtccgcgc gctctagcct atcgatgttc acttgaaggc
    27841 tggagacctc gcgggtctaa gatgatcagg ataaaggtga cgtgttatgt atgaagaacc
    27901 ctatcggtgg atcgaggcgg tcgggaatcg gcggcagtat ctcgatgaac agtttgcgat
    27961 gggtgcgccg gtcgtggcgt tggcctacgc cgacggcatc gtgctgttga ccgtgagccg
    28021 aggcacgccg aagctctatg agatttatga tcgcattgcg ctcggtggca tggggcatcc
    28081 ggccgatctg gagaaattac ggttctcgct cttggaaatg gcccatgtcg aaggcttcaa
    28141 tcgatcgccc tccgatgtga caggtgcgag gctgatgaag tacggcctgg cgccggccat
    28201 caagcaggcg tttgaagaga tttacaaggc gcccttcatc gccaaaattc tcttggccga
    28261 attgggcgtg aaaccggaac gggatctgtt tctgaccatc aactacgatg gcaactttga
    28321 ggaacggcgg ggctgggctg cgttgggtgc gacgtccgcc gtgcaagttc aaatgggtcg
    28381 gtatatcgac gcacagccgg ggtttgccca ggcttcattg gcgcaggccg tagaaaccgg
    28441 tctctgcgcg tgggcggtgg gatctctggc gcaaacctcg tccggtacat cggatggcga
    28501 ctcctctgtt cgcgacgcgg cagaaaaacc ggacgctgaa gccttgcccg acaaaacggc
    28561 cttgctggct catgtgcggc agaccgtggc cgaaaaatct ctggaatgcg tgctgctgga
    28621 acgcaatggc cctggttcgt ccaaatatcg gacccttcgc ccggacgaat taggccgcgt
    28681 gctgccgtcg gcggttcgag cctcacagtg atctcctccc gaggtaagac ggaacaaatg
    28741 ggcttggcat cgatcacgtt ctggagcccg gtacccatag ttggctgaat atgctgaatc
    28801 gtattttcgg gatcgaaacg gaatatggcc tcctggtcaa tcaggatcgg cctgaccatt
    28861 cgccttcctg ggtggcgcag cgcatccggg accacatttt tcacatcgcc cgtcgcggcg
    28921 tgctcgatct gcaccatcgt gggcatgatg agccgccagg caacgggggg tttctgacga
    28981 acgccgggcg catctacatc gacatgggcc atttggagta tgcctcgcct gaatgcacga
    29041 cccttgcgga tctcgtggca tcagatcgcg ccggagaccg gatcattcaa caggccgtga
    29101 ctgctttggg actggacgag acggtctcgc tcatcaagaa caatatcgac cacgaaaccg
    29161 atgcgacgtt cgggtcgcat gaaaactatt tagtcactcg ccagttcccc ttttctcggc
    29221 gcggtcttgg tccgctggtg actttcttgg tcacccgtca agtgtttacc ggcgcggggc
    29281 ggatcggttg cgccggtgat cctaatgaat gggtgcaaat cggcgggctc atcttgcatc
    29341 gtccggggtt gcgggacgcg caggatcgat cgattgtccc gtttcagatc tcccagcggg
    29401 ccgattatat cgtgaacgat ttctttgaat gggtgcagca caatcgggcc atcgtcaata
    29461 cgcgcgatga gccgctggcg gatcccaatc aataccgccg catccacttg ctctgcggcg
    29521 attcgaatat ggcggaatat gccaccgcac tgaagatggg cacgacaggg ttggtcctgc
    29581 aactgatcga ggcaggccag gctccccgtg gattgagtat cagcgagccg gttgaggcgt
    29641 tgcaggacat ttcgcgggac ccggatcgcc agtggattgt ccggctggaa tccgggcagt
    29701 cgatctctgc gctcgatatt caagaacagt ttcttgccgc cgcgcagcga cattgcaaag
    29761 gccaggacga agagacggat tgggtgctgg agcaatggga gtcggtgttg accgacctgc
    29821 ggcacgggta tgaaaaactg gtgggccgga tcgactgggc ctcaaaactc tggctattgg
    29881 aaacctaccg ggaggcggaa cagttggcgt gggacgaccc gatgctgaag agcctcgatc
    29941 tggaatatca caacctgcat cccgagcggg gactctgcta cggccttgaa gaggaggggc
    30001 ggggaccacg cctcacgaca gacaagggcg tccaattagc gcaggaccac ccgccccgca
    30061 atacgcgggc cttcggccga ggtgaattgg tgcggcatct gttgacgtcc acggcgactg
    30121 ggccgggggc agatgcggcg catagttttg gtgaccatgc ctcctgtgaa tacattatca
    30181 actggtccaa ttttaaattg cgtgataccg agccgttttt tatggccgac ccgttcaaaa
    30241 cctacgtgca ggacgtgcga agccatctca gtgcacgcta gttccggcct accgcttcca
    30301 tcccactcgc tccatcgcac ctttggacac atcgaacgca aagcgcaact gcaccgcaaa
    30361 gtacttttga accagccctg attgatccag cggccgatct tcctcgcgat agaaggccag
    30421 ttccatgtcc tgccagcgca cggcaatacc gacgatccag tcgagctcgg aggggttcag
    30481 ggtctgactc gcttcgcggt cggtgaagag gtttacgtcg ccatacagca cgaccttgtt
    30541 cttatagaca tccaggtcgg catgggcgac ataccggaac aaggcgcggc cggtattatt
    30601 gggtctggcg aagtactggc tgttatgaaa gagccaccca gctccggcat acgcggtgaa
    30661 attctgatga ggaaaggtgc gccgccacca ggtggaatcc tgaatggcct gaaatcgtgc
    30721 cgtcacgagc gcgtcggcat aggcttgctt caagccacgg cggtcgaggg gcgcatcgcg
    30781 ttcatactgt aaccgccaat tgaatcggtc cagcacaccg gtgaatgcga aggtgccgtc
    30841 ccactcagag agtttgatcc acccgtcggt gcgatccgag aaaaagttct gatcggtata
    30901 aaaggtaagg tattgcttat agaggtctgt ctccagatgc agcatgtgcc gcatgccgac
    30961 gagaccggta ttgtcagggc gggcggcaaa ggtcggattc ttcacaaacg cccccgtcag
    31021 gaagtatccg ccgaacaggg tttcctcctc ctcccgtgct tcgtgttctg gttcatccat
    31081 ggagggaagt ggccggccgt atttttcgag accgtaggca ggagggggcc agtcagcgac
    31141 agattgcagg caccaggcca tcagcagcag gcccaggatc ttgcgaccgt tgaagcgccg
    31201 ggtcttcatt cctggcaatt cgcaaagatt tcttcacaat cattggaggc atcaacagaa
    31261 ctgggcacga aatggtatcg gacccgatcg acccgctgat ctttgaggat cacggtcgcc
    31321 cagcagccat gatggacggt tggccggctt cccttcgatg acaccatgga ttcctcgagg
    31381 aggggggcct cccggtaata gcggagcatg gtgaccggcc cgtcttcacg cttctgtaac
    31441 ggattcccgg cgcaggccag tacttcttga agggtttttg cctgaagtgc ttgctggtga
    31501 gggtaccctt ctgtgacggg cgggctggtt tgacaaccaa gcacgaagcc ggtcaggagg
    31561 atgttcatca agccaatgta gagccggacg taggcagtct gcatgatgag tgacagggct
    31621 agagagccag gccctgtggg taacataact ctttgtttca aagcaaccga acagccggcc
    31681 tggactacgt ggaccccgtt gaatcccatt cgctggaaag actgccagca gcgtaggcgg
    31741 ctgatgtccg aggatgcgga tcagctccct ggaaaaacgg gccttgtcgt tgtcagtgca
    31801 agggagtgga tacggtaggt ctcggaatta cgtatgccag gtatccgcgt cagtatcatg
    31861 acggtcgtca gtgctctgac tggtcctgtc agcgggttgg gggccgtagg cggcgcgacc
    31921 ggggatggga agccctgcag ctaacaacta ggttatttct tgtaggccgc gggatatctt
    31981 ttctactgat gagttgacaa ttatgtctaa tcacctagaa taagcttcga atctcagaca
    32041 gattggctag tcgctgtgtg cctacactga ggctgtgcgt gccgcttatc cttccccgtt
    32101 ttgatcatcc gacatcctcc cgctgttttg gaatcgttcg tgcgcttgca agagtccagc
    32161 ctcgcctcga cgctcgatgc atgacggggc gagatggcca cgggttactc accacgatgc
    32221 tgtggcagga ccgacaccag gactcaaact agcgggagga aatgtatgtc gtctgatagc
    32281 actgcgcgta atgtgactca gccgttatct gccctctgcg ggcatgagga tagcctgaac
    32341 gttccggccg tcgagagaaa gaaaacgcag gacgcctggt cgctttctct cgccgaacgg
    32401 cgagcgtctc gtcgtctggc gcttcgcctc tccactcagt tgttttttca gggacacacc
    32461 tggaaggggg tgaccaggag cctcagtctt ggcggcctct cgatggactt tgtctccgac
    32521 attcctgcca tgctgaatca gcggatgatg ttgagcttta gcccggatga gcgcgggttg
    32581 gacagcgttg gtatcgtgtg cggtattcgc tcgtccgagg atgtgcacgt acctggtcgc
    32641 actcaagcgg cggtgacact ggccatccag ttcatacacc tgagtgacgc agatgaacgg
    32701 gttgtggcat cgctcctcag tcatggcgct actgcgccaa gacacatgcg tctggcggcc
    32761 gccttggtcg tgcaggaagg agaggagtcc ctcattgata cgggttcatt acagaccgcc
    32821 gctacgcctc cggcctccat gcaactggta cgagggccag aacgcccgag ggaagagcga
    32881 cggcgacagc agcgggtggt tgttggattg agcacggaag tgagtcttcg aactcgtggc
    32941 ggggatcggc cgttgtccgc tggcttgacg acggacttgt cgcccaatgg cgcgtgcgtc
    33001 cacctatcgg ctgaagaaga tctcttggga tcggagctcg aattacgatg gttgccatcc
    33061 gctcccctcc agggatcttc gaccgattct ttgtcggcaa ccctgtgctc ggtggtgggg
    33121 gaagtggtgt ggaccagacc agttgcaccc gaggctcgcc ccgggtgcgt ccctgtggca
    33181 gggcgtaccg tgctggcagg ggtgcgcttt ctcccggttg cgaaggaagc ggaagatatc
    33241 atcgaacatt tcctgggaca ggtgtcgatg ccaagccctg aaacaaaagt gggcagtccc
    33301 accgtgctca gtgagttctc agaatgtttc cgaccatccg gcctccggat cgtcatctgc
    33361 catgaccggc cacaagttgc cccggcagct gacgccccga ttatcgtatt ggcgccggga
    33421 tatggggaga gcaagcgcga ttatgtgccc ttggcgtatt acctggcggg caatgggttc
    33481 cacgttgtcc gttatgacaa tgtgaatcat gtcggggaaa gtgacggact ggtgacgcaa
    33541 ttccggctgg aggacatgga ggcggatctc gaaaccgtgt tggaccatgt cgccaatcaa
    33601 tggccgggac gaccgatcgg cctcgtggcg accagtctgg cgggacgcgt cgcgctgaag
    33661 gtggccggca aggtgtcgca tgtgcggctc ttgaccctca tcaacggaat tatggatgtc
    33721 cggcatacgc tgcaggccgt ccatcaagag gatttgattg gagagcatct gtcaggtgta
    33781 cacaaaggcg tggtgaacat catggggctc accatcgatg cggatcgatg gttggcgcat
    33841 gcggtggagg gcgagtatgc cgatctcctg acgacgcagc gggatgcctc gcgtctccaa
    33901 actccggtcg tcctgtttca tgccgagcag gacgcctggg tcgatcccgc ttctattgag
    33961 cgggtgactg aggcaatcgg tccgtatttg cgccactcct tcgttgtgcc gggcgcatta
    34021 catcgcctgc aggaaagtcc acggaaagcc cgtaccgtgt accggcaaat cgccgtctgt
    34081 tgccaacagg aactctggcc ggcgcggcgg ccggagcgaa tggtcgaacc gtcccaccgt
    34141 gaaatcggtg cgcagaaccg gcttgagcgc gaacggagca agaagcggaa gccgatggga
    34201 aagtctgatc acgtggcgtt ttggcaggag tatttacaga actttcagac gattcccaat
    34261 gtggccgatt tctggcggct catggatcat ctctatcgat tgatgggcga ttgccacaag
    34321 ggcgagcgca ttttggatgc cggttgcggg aacggcaatt tcggggtctt cctgcaattg
    34381 aatcaggcgt ttcggcagcg gtatggcaag cgcggcgatt ttcgctctcc cgattacgtc
    34441 ggtctcgact tcgtgccggc tgcattggct caagccatga gcaatttcga gcaggtcggt
    34501 gcgacgctgc aggggcagtt ttatgaaggg ctgcgggcct atgagcccat gtcgatgcgt
    34561 gtctgccagg ctgacctgga aagcgcgctg ccgtttcccg atcactcctt cgaccgcgtc
    34621 gtctgcaacc tggtgctggg ctacgtacgt gacccgctgt tcactttacg tgaatacctg
    34681 cgcgtgctca ctccacaggg gcggttggtc atttccaatt tgaaaccgta tgcggatctt
    34741 tcggccattt atcgcaattt tgtggatacg gctcagacga aggatcaggt cgaggaaggt
    34801 cgtcgcctgc tggacaattc tggtagaatc aaggaacgcg aaggcgaagg cacgtttcat
    34861 ttccactacc agactgaact ggaaagtcta ctgcgagccg cgggagtctc acgcccgcga
    34921 gtgtattcca cgttcggcaa tcaagccctc attgccgtgg ccgaaaaggg catggcgcac
    34981 gtcacggtcg ctgcatgatg acacggctcc ggtcctcccg gaggacttga tatgaaagtg
    35041 ctcgcaatca gcgcgctggt caatgcgttg gctgcgacag ggctcggaat gttcgtctat
    35101 ttccgggatc ctgccgctgc gcgcaaccgg atctacgggc tgtactgtct gagcatcgcg
    35161 ttctggagcg tgttttactg cgggtggcag ctggcggagt cgaaagatct cgcgctgagc
    35221 ctgttgcgcc tggtcatggc cggcgcgtct ctcatcccca tcctgtattt tcaccacaca
    35281 gtcacgtttc tcgacattgc ggttcgccat gccaaggcgc tgaaaatcgg gtatggacta
    35341 gccggactgt tcctgctggt cgatacgact ccctggttgg tggccggcgt gcatccggcc
    35401 atgtcttttt ccttctggcc ggtaccgggc ccgttcttcc atccatttct cgcgtatttt
    35461 ctctggtacg tggtctatgc cacctcactt gtcggtgtcg cgcttcgcga tgccaacggg
    35521 attcgccgcc atcaatatgc ctacatgctg gcagccagtg tcattggcta tgccggcggc
    35581 gcgacgaatt tcccgctctg gtacggggtg ccgctgttgc cctatgggac ggtgctgatc
    35641 acgatgtata cggcgttgat ggcgtatacg atcgttcggt atcgcctgat gaatatcagc
    35701 gtcgtcctga acaaagggct gggatatgcc atcgtactgg cgattattgt ggtggtgacc
    35761 tctatcggcg ccgtcctcag caatcgggcg accggacact ccactcctcc tttattagcc
    35821 ggtactctgt ttttgatctg tgccttatgg gtgctcagca ataatccccg ctcatcttcg
    35881 aatacggtgt ttagcgccgt atgcggtgct gcgtgcctgt ggctattcgg ctgcttcatg
    35941 ttgttctctg ccacccacga agaggaggcc ctgttctggg tgcgagtcat ttatacggga
    36001 gtcgtatttt tgcccgccct gacctatcac tttgcgcaac ggcttgcggg aggggcggtg
    36061 ccggatcggg cgattgggtg gaactacgcc ctcggcgggc tgttctggag tctcttgttc
    36121 acgacgtatg tggtggatgg acagtatgtc tattattggg gccggtatcc gaaggccggc
    36181 gtgctccacc cctggttggt cgcctatgtg gtgggaggcg gtgggctgac ggtgtatcgg
    36241 ctctaccagg gctatcggtt ccatcaaaca tccgctcctc ttttaggcgc ccaattaaaa
    36301 tataccttcg tggcgctgtt actcggcttc tcggcctccc tcgacttctt gcagaactat
    36361 ggcatcgggt tctatccggt tggctatttg ctggcgggac tgtccgtcac ggtcgtcgcc
    36421 tacgccatcg cgcgatacga attgatggat atctcgttcg tgccgagcag gccgaaggtc
    36481 atgttatcga tcaagctggc gggtctgatt ccggcttaca tgatgatatt gctggtgatt
    36541 cgaattttca ccggcacgtt ccactatctg cttgccggag tgctcttcgg gctctttgtc
    36601 attgtgtcga gtggtttggc caatctgcaa aaaggcgtgg agcgtgcgat cgggcagacc
    36661 ctctttcggg agcgctatga tgcctacgac acactcgtgc agttttccaa gtccctggtg
    36721 gcgatccttg agctgaaatc gctcacaaag gaaatcgtac agacgctggc ccgcgtgatg
    36781 aacattaaga ctgcgtcagt gtatgtcctg gacaaagaac agggggtgta cagtttgaca
    36841 gcctcctatg ggtttgtgac gcgagatgtg attgtccctc ccatcaagat ggagagcgaa
    36901 ttcccgcggg ccctgttgcg gaccgaaacc gctctggtcc gagaagagat tgaatacgac
    36961 aaagcggatg tcgactacct gcgattgctg gatacattga aggggttgga gtcggaagtg
    37021 tgtatcccgt tggtcagcaa ggagcggctt gtcggcttct gtaatttagg gcgccgtgcc
    37081 aatcatggca tgtattcgac tgaagagttg gcgctactga aaacgctggc tcaacatgca
    37141 gccatcgcga tcgataacgc cctgctgtat gaagacctgc ggcggtccca gttgttgatg
    37201 cgccgtaccg accgactccg gtcgctggag accatggcgg gcggatttgc tcatgaaatt
    37261 cggaatccgc tgacctccat caagacgttc gtgcaactgg ctccagaccg gcgggatgat
    37321 gtggaattta tggagcagtt tagtcaggtg gtctgtgagg atgtcgagcg tattgaacgc
    37381 ctggttcacg agattctgga ctatgcacgg tatatgacgc cgaaattgac gcaggagagc
    37441 ttgaacgacg tcgtgtcctc ctgcttgtat tttatcgagg tgaaggcgag cagcaaagcc
    37501 atcactattc aaaaagaact cgcgccggac ttgccctacg tcaagctgga tcgtcagcag
    37561 attaaacagg tgctgctcaa tttatttatc aatgcgatgg aagccatcgg gggcgaacag
    37621 ggcggacgcc tgtctgtccg tacacgaaag ctcgtcaaat ctgtgaatga gccgtgggtg
    37681 cagatcgagg tggaggatag cggcccgggc attgaaccgc acgatctgga tcatattttc
    37741 gatccgtttt ataccaccaa gcatgaaagc ggcgagcgtg aggggacggg cctggggttg
    37801 accattgctc accaaattgt ccaggagcat ggcggctatg tggaagtgtt cagtgaagtg
    37861 gggcacggca ccaagtttat ggtaagcctt ccggtaaacc cgcccatggc ggagtggcag
    37921 gcggcacaac cggtttatga tgatggccgg aaacttgccg gatcattttt ggccaggcct
    37981 catctcggtt tggagcaaca gttcagcaag ccggactcac ccctcaaaac ccggacgtga
    38041 gacaggaagt gcacctgcct gagctgagcg tgctgtctca ggttccgcac aaatcattgg
    38101 aggcctttgc ggatggccgc taagacgatt ctccgtctca attatgcccg agaagaaagc
    38161 cactcgataa ttctgttttc cgcccaaaac cgatcttctc cataccggcg cttctgaaaa
    38221 aacccacagt gtccaccgtt ccgtagcatc gcgagcgtaa tgtagcgatt gtttttgatc
    38281 cccgggctgt cgaacattga tgccggaata aagggatcgt cctgggcggt gataatcagc
    38341 gtcggcaccg caatttcgga tagcacgtgg cgagcgccgg cccgattata ataatccgta
    38401 gcgtctcgat atcctccgtc gcgtgcggtg tacgtgtcat cgaattgagt aatcgtcttc
    38461 atgaggcgta gaggcgagag attccattgg cctggaaaca gagcggcttt ccgttgcatt
    38521 cgggccttga gactcgaaag aaagtgcgca tgatacagcc agtttctcgg ctcttccaga
    38581 gcagccacac attgcgtggg atcaatattg ggactgaccg cgaccacgcc tgccagggcc
    38641 gcttccgatc gttgcagttc tcccgctgct tttagcacca gatttccccc catcgagtac
    38701 cccacgagcc agatatggct caggccatcg cgctcccgca attcccgtat gatggcaagg
    38761 taatcattgc tgagtccgct gttatagaga gttggagtca ggtgatccgt gcccccacaa
    38821 gtgcgttggt tcatccggat gatgttgaat cctgcgcaga aggctttggc cgccatccct
    38881 cgcatatagt gagaatcggc cgatccttca aggccatgaa ccaggaggac ggttggtgcg
    38941 gtcgtggggt catcctgcca atgacaaaag ccgagaagct gggtgcctgg ttgagtcgtg
    39001 aagagacgtt cacagcaagg aaggttctct ttccatctca ggcgaggcca atatcgcgga
    39061 atcagcgtca tcaaatgcgg gtttcggagc agcaatgcag gggtaaatcc gttgaggatg
    39121 gtcatacgtt agtcatcata gccaaaagtc gatctggttg cccaaatcaa attttccttc
    39181 agctgaaggt gctgcgtcct gcttaaggcc tgaaagcaaa atgccgataa cagatgctgg
    39241 atccttgttt cttacaggag gcagctatgc tgacggcaat cggcgatgtg atgaggcggg
    39301 tgtacgagcg cggctggatc accaccagag atggcaacat cagcatgcgg aagcgggatg
    39361 ggaagtacct gtatatcaca ccgtctggat ggcggaagac cattgtgcat cctgaacatg
    39421 tggtcaggct ggaagttgtg acagatccaa ccagcggtca tttggtgcca tctgtacgag
    39481 aggggcagca gccttcgggc gaactttgga tgcattggaa tttgcagcgg gacaccaaaa
    39541 aaacgcgcac ggttgttcat gtgcacgcga cccatatcgt tgccgccatg tatgcgggaa
    39601 ttgatttaca ggcgatgagc gctgaattcc ctgagatttc ccgctatacc cgtgttggcc
    39661 gtacagttcc cgcattgcct gcgttatctc gtgacctggc cgatgtgacg gcggaatgtt
    39721 tagggctgag aaaagacggg accctggagt ttgacatcgt agggcaaaag aatcacggcg
    39781 tgtgcgccgt cgccacagat ccgtgggcgg cctatgagca cattgagcgg ctcgatcaca
    39841 tttgtgaaat cgtgctgaag agcggagttg ctgcacggtc atctggaaag ctatgacacg
    39901 gtagaaagtc taacgctcac gcttcagata gaataagggg ctgtcggcag cataccgaca
    39961 gcccctttct atgtgtctag attggctcca cgg
//
LOCUS       DAOQDS010000040        37969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_360_length_37969_cov_18.052645, whole genome shotgun sequence.
ACCESSION   DAOQDS010000040 DAOQDS010000000
VERSION     DAOQDS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 37969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37969
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_360_length_37969_cov_18.052645"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..2459
                     /locus_tag="PK589_11285"
     CDS             30..2459
                     /locus_tag="PK589_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNJ20230.1"
                     /translation="MFERFTDKGRKIIILAREEAERHQNDYLGTEHLVLAILRETDGI
                     ALMILKKMGLSTEQIRLEIERNLPGGGTTMTFGEIPFSPRVKKVIEYGVEEARLLGHN
                     HIGSEHLLLGLLREEEGIGGKILRSLGANLLTARQLTVTFLRKSAPRERDRKSNTPAL
                     DEFGRDLTQLAQEGQLDPVIGRADEIERVLQILSRRSKNNPVLIGESGVGKTAIVEGL
                     AQRIIASEVPDNLLSRRVIALDLGSLVAGTKYRGQFEERLKVVMKEIVQAGNIIIFID
                     ELHTLVGAGAAEGSIDASNMLKPALSRGEIQCIGATTLDEYRKHIEKDGALKRRFQPI
                     HVQPPSTDETVRIIQGLRDRYEEHHGVEITEDAIVEAVKLADRYITDRFLPDKAIDLI
                     DETGSRAKLQTYALPTELKALEQELKKISREKELAISMQNFEEAVRHREEEERLRKLL
                     DESKREWKKNQEKHKPTIGKEEVAYVVSKMTGIPLFKLEEEESNKLLRMEEFLHKRVI
                     GQNEAISAVARAIRRSRAGLKEAKKPIGSFIFLGPTGVGKTELARTLAEFLFNSEDAL
                     IRIDMSEYQEKFTSSRLFGAPPGYVGYEEGGQLTEKVRRRPYSVVLFDEIEKAHPDVF
                     NVLLQVLDDGVLTDSLGRKVDFKNTVVIMTSNIGTKLIQKGVSLGFQNDEKSGQALRK
                     KDEVMGELRRSFSPEFLNRIDEIVVFHSLEKEHLIHILDILLHELNLRLSDKSVNIEV
                     DDEVKQWLIKEGYEPLYGARPMRRIIQRAIGDPLSEELIKGRFKDNRKIKVVLRDGAP
                     AFIEQEAMAGV"
     gene            2708..3778
                     /gene="tsaD"
                     /locus_tag="PK589_11290"
     CDS             2708..3778
                     /gene="tsaD"
                     /locus_tag="PK589_11290"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNJ20231.1"
                     /translation="MTHAASATILGPILGIETSCDETAAAVLDGEGHVLSNIISSQQA
                     VHERFGGVVPELASRAHIQNIDQVTGRALEEAGLGWRDLRAIAVTQGPGLAGALLVGL
                     SYAKSTAYALDIPLVGVSHLEGHIASAWINRPAFPRDCVVLIASGGHTHLFHAEEKGG
                     FHLMGRTIDDAAGEAFDKGAQMLGLGYPGGPLIDAAARKGRPTAVRFPRSRVRTGQFD
                     FSFSGLKTALLYHLQGMSLEAIAEQQTDLAAAYQEAIVDVLVRQAFAAVERAGVSALA
                     VVGGVSANSRLRARLAERAVKEGIELSLPALSYCTDNAAMIAAAGQRALLQGNVASFD
                     CEAMPDCALPAWSGSGVVRRTS"
     gene            3729..5498
                     /gene="hflX"
                     /locus_tag="PK589_11295"
     CDS             3729..5498
                     /gene="hflX"
                     /locus_tag="PK589_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNJ20232.1"
                     /translation="MRCRPGRGAGSSGAPPNVLIARSPVIATIHGHVSGLKASHLAAL
                     ERLYRRRIPASVVLTAELARTTTQLSREIRRPIGLLITRRGAIEQVLVGAGCAPTFES
                     LAKFRVGSHSLRGLRLIRTHLHDEPLSQDDLTHLALLRLDLIGALGVTETGEPGLLHL
                     AHLLPPNQQGEVCRLLKPVPFHDLELQLDTFLHTLESDLQRSDTQHTVTGGQESAILV
                     SAAPKGHAEQDEHLEELTELAESAGVRVLDRIAQRTHEGYERYLLGKGKLKDVVMRSL
                     QKGADLIIFDQDLAPAQARAISEVTDLKVIDRTQLILDIFARRAHTREGKVQVELAQL
                     RYLLPRLSGHGTSLSRLGGGIGTRGPGETKLETDRRRIRDRIAHLEREIDHVARHQDQ
                     RRARRVRQGLPILSIVGYTNAGKSTLLNALTQSQISAQDRLFETLDTTSRRLRFPHDR
                     EVIVTDTVGFIRDLPKDLVGAFRTTLDELRDADMLLHVLDASASNLDQQIAAVDAVLQ
                     SLQLDTIPRVMVLNKCDRLSPREADVLSQRYHAIAISALDRETLRPLIAHLEALLPAV
                     PSPPSHEADTELPPQERALASHS"
     gene            5533..6204
                     /gene="nth"
                     /locus_tag="PK589_11300"
     CDS             5533..6204
                     /gene="nth"
                     /locus_tag="PK589_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNJ20233.1"
                     /translation="MKAATAHHVSAQPDKRRLARMLRALRATEPARKVELDYRTPWEL
                     LVATILSAQCTDQRVNQVTPALFRRYRRPQDYAAADAAELEALIRPTGFFKTKAKNLI
                     HCAKAVVEIFHGQVPETMEALTGLPGVGRKTANVLLGNAFDKPAIVVDTHVKRVAGRL
                     HLTRHTDPEKVEMDLQQLLPAAEWTEGSQRLLLHGRYVCLARTPKCGNCAIYTDCHWE
                     GKLTR"
     gene            6201..7079
                     /locus_tag="PK589_11305"
     CDS             6201..7079
                     /locus_tag="PK589_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNJ20234.1"
                     /translation="MIRSMTGYGKKDGTSQQATVTVEIRSVNHRFLEVAVRVPRSLAQ
                     LEDPIRKAVQQRCLRGRVDVSVSVQTAGGSLKTVHIDQVLAKQYHTALKKLQKTLGLR
                     GGVDLSMVAGFRDIVSLTDEPVDTDHLGKTLLRVLGGALTELEKMRKREGEALTKDLT
                     LHLDAIREAQSIVAKKAPDLAKIAFDRMKARIETLLQSEVPDPARLQQELAVYADRSD
                     ISEELVRLESHMVQFDQQLRSQESVGKTLEFLLQEMGREVNTIGSKANDAEIAALVVR
                     MKAELEKLREQVQNVE"
     gene            7110..7826
                     /gene="gmk"
                     /locus_tag="PK589_11310"
     CDS             7110..7826
                     /gene="gmk"
                     /locus_tag="PK589_11310"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNJ20235.1"
                     /translation="MSTAPVPSHDKPAQVRRGILFIISAPSGSGKTTLCKQITANVPG
                     LWHSVSYTTRKPRPGEIDGQDYHFLDEASFRQMIDRNEFVEWAHVYGNLYGTPRKELT
                     EKMEQGIDVLLEIDVQGARSVKKKFEDGVYIFILPPSFDTLRTRLQNRAGDSPEEIQR
                     RLQKAKEEVWSYREYYYIVRNDDLKQSLKELESIFLAERIKTKRLNMTWLEEKFILDK
                     EGKQGNSSSAPASKEHMHHG"
     gene            7819..8181
                     /gene="rpoZ"
                     /locus_tag="PK589_11315"
     CDS             7819..8181
                     /gene="rpoZ"
                     /locus_tag="PK589_11315"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNJ20236.1"
                     /translation="MGEMLSLLPEYATGEFDSRHRLVIVAAQRAKQLVQGARTTASSK
                     FTKETSIALDEVLRHNVKFLVGKEARDAIKESKRVKEGETERLAMMTGEDAKEIKKEL
                     SVYVDDTAKPVAAPEAEE"
     gene            8191..9405
                     /gene="coaBC"
                     /locus_tag="PK589_11320"
     CDS             8191..9405
                     /gene="coaBC"
                     /locus_tag="PK589_11320"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNJ20237.1"
                     /translation="MHTTGPSLSGVRLVLAVTGSIAAYKAVSLLRLLRREAATVNVVM
                     TAGACRFVTPLTFEVLSGTHVATDLFEAHQEMLHLSLPEQAQAIVIAPATANCLAKAA
                     LGLADDLLSTMLLTTQCPVIFAPAMDGDMWQHPTVVEHVATLRARGAIIVEPEDGPLA
                     SGRQGQGRLADEERILAAVHHAVHPRRDWSGRKVLISAGPTQEAIDPVRFLSNRSSGK
                     MGYALAEAARARGADVVLVSGPTSIPVPAGVEYCPVITAEEMHKALTTQLDWSDTVIM
                     AAAVADFRPARPSAHKIKKRRGPVTHLDLEATDDILCELRDRRTSQVLVGFAAETDDL
                     LAHAKEKLHAKGLDLLVANDVTAPGSGFGSDTNRVWLLAREADPEELPLLSKRDVADR
                     ILDRILTVHTGR"
     gene            9448..10134
                     /locus_tag="PK589_11325"
     CDS             9448..10134
                     /locus_tag="PK589_11325"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ20238.1"
                     /translation="MSSPLRIPPAIAAEIDRLATAVAKDPRSKDFLPLADEYIKAGMW
                     QEAAAVLEDGLKVYPGFVTAMAALGRVYDQLGQAEKAKVILEEVVKQRPDNLRAHRIL
                     AKLYHAEGQADLALRSCTAILNANPFDEEALAVKRSVTGAPDEPPPSKREKKRSLAEA
                     PVEKITAAVPPAPAPVVLVSESAAPTVSMPDPTPPQSPPSPAVQHAAAIARLEAWLST
                     IQAKRHPAAR"
     gene            10257..10754
                     /gene="aroQ"
                     /locus_tag="PK589_11330"
     CDS             10257..10754
                     /gene="aroQ"
                     /locus_tag="PK589_11330"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNJ20239.1"
                     /translation="MLRLLVLHGPNLNLLGTREPSVYGQMSLSDIDKSITRRSGELGI
                     AVQTKQSNSEGELVTWIQNARGHFDGIIINPAAYTHTSIAIRDAIAAVALPTVEVHLS
                     NIHQREEFRHHSFIAGVAIGQIAGFGPTGYLLALEALTVHLGAKRSSSRTRADVRKKP
                     AASRR"
     gene            10780..11340
                     /gene="efp"
                     /locus_tag="PK589_11335"
     CDS             10780..11340
                     /gene="efp"
                     /locus_tag="PK589_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNJ20240.1"
                     /translation="MISTAEFRNGSPLMVEGQPFYIVEFQHVKPGKGGAFVRTKLKSY
                     LSGNVLDRTFRSGEKFDTPEIEECDMQFLYATNDSYTFMDTETYEQSTYEKSQLGSNA
                     DLLKENMIAKILIYEHKPITVVLPNFIELKVVDGEPGVRGDTASGGSKPVIVETGATI
                     KVPLYLEIGEVIRIDTRTREFVERVR"
     gene            11337..11915
                     /gene="accB"
                     /locus_tag="PK589_11340"
     CDS             11337..11915
                     /gene="accB"
                     /locus_tag="PK589_11340"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNJ20241.1"
                     /translation="MSPSRKKTGKSRTAAKPIVLPSAFAPRTSAADQMILNPDQAAQI
                     QQLADLLKRNHLTELEIERSGMRIRIRHEPAVRATTTHTVEAVHTQSATSGTAPAAQA
                     RPSAETDGQVTITSPIVGTFYRSPSPDADPYVEEGDYVKKGQVLCIVEAMKLMNEIES
                     EADGRITKILAESTKPVEYGQPLFLIDPSATP"
     gene            12093..13442
                     /gene="accC"
                     /locus_tag="PK589_11345"
     CDS             12093..13442
                     /gene="accC"
                     /locus_tag="PK589_11345"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNJ20242.1"
                     /translation="MFKKVLVANRGEIALRVIRACKELGIKTVAIHSEADAASLHVRA
                     ADEHVCVGPPEAALSYRNIPNVLSAAEVTGADAIHPGYGFLSENAHFAEVCESIGVKF
                     IGPTSENIALMGDKSKAREVVAKKGLPVTPGSPGELRSEQDAQEAAKNIGFPVIIKAS
                     AGGGGRGMRVVNKPEELTRAFQAAQAEAKSTFGHDGVYLERYFLEPRHIEVQIVADNR
                     GHVVHLGERDCSIQRRHQKLVEETPSPAIDERLRREIGRTAVEAVKAIRYCNVGTVEF
                     LLDKDRNFFFMEVNTRIQVEHPITEMVTGIDLVKEQIRIASGLPLSFKQPDIKLNGHS
                     FECRINAEDPEKFTPCPGQITKYSAPGGLGIRVDSAMEPNAVVVPYYDSLIAKLITHG
                     RDRQEAMARMRRALDEFVIEGIKTTIPLHRKIFNDPDFQKGHVSTTFLDRFLAGQSA"
     gene            13515..14129
                     /gene="thiE"
                     /locus_tag="PK589_11350"
     CDS             13515..14129
                     /gene="thiE"
                     /locus_tag="PK589_11350"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNJ20243.1"
                     /translation="MLDPTVRPERPLTDVLRVAAASGIRLFQYRDKQASMKDAYRQAL
                     ALRQVATEVGATLIINDRCDLALAVKADGVHLGQDDLPLADARQILGPEAIIGLSTHN
                     AKQVREAVALQPDYIGFGPIFGTTTKTDHDPVVGLEGLRAARTLTTLPIFAIGGITSQ
                     HVTDLTAAGADGVAVISAILKAQDLEAAIRSFQPPPSTQARPSH"
     gene            complement(14077..14823)
                     /gene="bioD"
                     /locus_tag="PK589_11355"
     CDS             complement(14077..14823)
                     /gene="bioD"
                     /locus_tag="PK589_11355"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNJ20244.1"
                     /translation="MIGTTGVFVTGTDTGVGKTLVAAALVNRLVRMGRSVGVMKPVET
                     GVDAGLSDRTDAARLMAAAQVTDSLDLVSPYRFPAPVAPLSAAAAQGRVIELGDIVGR
                     YEQLAARYPCVVVEGAGGLLVPTGSDWAMADLISRLRLPVLLVGRVGLGGINHALLTL
                     EALERRNIPVVALVLNETVAPRGSVEQEQAASTVALLRDRVSMPLIGPLPYQTRADCA
                     WPEVVENVAGSRPISDLAELVWKAAAGSSG"
     gene            complement(14847..15608)
                     /locus_tag="PK589_11360"
     CDS             complement(14847..15608)
                     /locus_tag="PK589_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ20245.1"
                     /translation="MQSEPQTSASSPRELRTVLVTGGSGGIGRALCLAFARAGYWVGV
                     HYGRHKEAAEETLRELSTDGGAGAIYQADIRSGLEIQAMIDAVLDRQDRLDVLVCNAG
                     VASSHLVLRCPEDEWLRIIDTNLTGTYRCLTAVAPLMIKQGGGSIIVVGSYAGLHGAS
                     GQAAYAASKAGLVGLVQTAAREWGPHNVRVNLVCPGWQPTALAGDAFPSTEQLSDHVL
                     GRLMNLEDVSRTICHLAQLPGMSGQIVNLDSRILY"
     gene            15715..16617
                     /locus_tag="PK589_11365"
     CDS             15715..16617
                     /locus_tag="PK589_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20246.1"
                     /translation="MVQNLFRSTLIAVGILGSAAILVGHDTASATEAGQAIAQSADYF
                     PDTIGTRWQYRGQISEGPLQTIENKYFSNVSSVTGTRTLKGGLVVTVFHDTNPGNHGP
                     SDSFYRRDAAGVVYYGSEPGTPLEKQLTPYQIIRFPMKVAQSFQQFNRTDIDFGSDID
                     GDGTNEKVDVEGLSTVLGQESITVPAGTYPDAVRVEAKMTLKIRLSSVDRTAVGTDVM
                     TAWFAKGVGLVKYIERQELAALKDDRGNITDITEELEEVSVKSAPGLQGRHESAPEGL
                     LADDTSNQKLFQVLFASGLRAYAR"
     gene            complement(16507..>17193)
                     /locus_tag="PK589_11370"
     CDS             complement(16507..>17193)
                     /locus_tag="PK589_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNJ20247.1"
                     /translation="DETADLDEAVTGVVQSFSGYAGQKCSACSRVIVVDSIHDAFLER
                     LADAVRSLRIGAADDPATQVGPVIDARAKQRILDYLEIGRREGRVLVDRSAEGPGYSV
                     GPVVIADIEPVHRLAQEEIFGPILAVMRVASFEQALEYANGTAYALTGGLYSRSPRHI
                     ARAREAFDVGNLYINRPITGALVSRQPFGGHRLSGVGAKAGGEEYLKQFLVARVVSEQ
                     TLRRGFVPTL"
     assembly_gap    17194..17293
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17294..19643)
                     /locus_tag="PK589_11375"
     CDS             complement(<17294..19643)
                     /locus_tag="PK589_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNJ20248.1"
                     /translation="MTLDPALLEPRIRTIGEDLARRSSGLSPGLFDSRWWSQAAINLA
                     MKDPAFKAQLFRFIDVLPSLRDDAQVVRLAEEYFGDMSGHLFGAQWGLKALASTKLGA
                     ALSGKAIRHQVEQMATSFIAGASIEQAVPALRKLWDDGRAFSVDLLGEASVNEREADA
                     YRDRCLAALRILGDVCPAWSPSPLLEHDHLGPLPRVQLSIKISALYSQLDPIDPESSY
                     RAVAARLRPLLNVAGTLPASIIFDMEQAETKDLILSIFMRLFEEEPYRTFAHAGIALQ
                     AYRIDTYDDVQRLLAWTTRRQVPITIRLVKGAYWDSDAIRYRQRGWPMPLFEQKSQTD
                     VNYESLVRLLLEQSHLIRPAFGTHNLRSLAVIEAVAEALGLPPVAWEYQMIFGMAEPF
                     QHAMSDRGRRLRLYTPVGELLPGMAYLVRRLLENTSNESFLRKEYVESQPLSLLLSPP
                     DGAPSHPAYSSPVEHKSSPYTSPTEFVNEPVADFSRASARTAMADAIERRRKHLGQRL
                     DLSTLAAHLPTGPDLSTRNPSHPEQVVAVVQSYQPADVAALTKIAGAAEESWTRRPVA
                     DRVAVIRKAASLMRDQRWDLAAWEVFEEGKPWREADADVAEAIDFLEYYAGEMARLDP
                     PPRLGRYPGELNEVLWNSRGVTVVIAPWNFPLAIPTGMVAAALVAGNPVLFKPSERSS
                     AMGYQLARILLDAGVPKGLLQYIPGGPELGRELVVSAAVRTIAFTGSKDVGLGIMQAA
                     GVQQPGQRFVKRVIAEMGGKNAVIVDETADLDEAVTGVVQSFS"
     gene            complement(19829..20656)
                     /locus_tag="PK589_11380"
     CDS             complement(19829..20656)
                     /locus_tag="PK589_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20249.1"
                     /translation="MLGTVTRKVLKTILSDTARQRILTVERVHREGGLFDADSIFYRC
                     VGTVVTWGTGSRVHSGPFAGMRYIQHALGVHSSAYCPKLLGTYEQELHPLVEALRTGH
                     PYAHVINIGAGEGYYAIGFARCLPQSRVTAYEADAESRVILGRMAEINGVAPRLTIRE
                     SCTPSGLQAAMAQPGKTLIVCDVEGAEQELLDPVAIPALTRADILVELHDFLIPGISG
                     VIRKRFENTHQITEIPALPRQLADWPLSLSLPKVYRRTALSEYRPGGMCWFWMKARG"
     gene            21064..21642
                     /locus_tag="PK589_11385"
     CDS             21064..21642
                     /locus_tag="PK589_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20250.1"
                     /translation="MHSTSKVICQMPPMPIETTHELIEIILAKWEGAIGEDYLGYKGH
                     VYRMFNFCLALHPCTEEENTKLAIAACFHDMGLWPDRTLDYLPASISHAAQYLSDSGR
                     EEWSEEMALMIGMHHKVRAYPDRRYPLVEVFRKGDLVDVSLGLLSCGIPRSYITDVKN
                     HFPNHGFHTFLLKTGRAWVSRHPFNPAPFMRW"
     gene            21821..22654
                     /locus_tag="PK589_11390"
     CDS             21821..22654
                     /locus_tag="PK589_11390"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNJ20251.1"
                     /translation="MADSSTQSVCWGTVALFTALTILTFVGVPLFAYYYDYTVLDWTL
                     LAILYVVTGMGITVGYHRMITHRSFESHLWVKICLLIMGGWAVQNSALLWCADHIRHH
                     AHTDEEADPYNASKGFWHSHVGWLFINTPYREDRFAAKLRTDPMILWQHRYYWVIVAS
                     GLLLPFAAGYLNGGLMSGLGCFLLAGVLRTVLVLNSTFTINSLCHMVGTQPHGTQDSS
                     RDSWLISFISFGEGYHNYHHTYSHDYRNGSKWYNFDPSKWLIYTLSLVGLTYNLHRER
                     T"
     gene            22927..23823
                     /locus_tag="PK589_11395"
     CDS             22927..23823
                     /locus_tag="PK589_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNJ20252.1"
                     /translation="MAEEPGGAPHQDEAAARPNIIPGVKHVVAVSSGKGGVGKSTVSV
                     NLAVALALTGAKVGLLDADIYGPNIPMMMGVEKTPEQKDGKIAPAESHGVKLISMGFF
                     VPEDTAVVWRGPMVHTAIQQLFRDVLWGELDYLLIDLPPGTGDAQLTLTQLVPLSGAV
                     TVTTPQEVALHDVRKGMMMFQKVNVPLLGIVENMSFFVCGHCGERTEIFSHGGGERAA
                     EKLGIPFLGRVPIDPVIRAGGDTGNPIVVAKPDSPQAQAFRDIAAKLAAALQTGGVSA
                     TKSRIESLLDKIKRPAADRKPD"
     gene            23851..24156
                     /locus_tag="PK589_11400"
     CDS             23851..24156
                     /locus_tag="PK589_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNJ20253.1"
                     /translation="MGELIRIAGTADVKPGAGMVAEVNGKAIAVFNVDGTFYAIDNTC
                     VHRGGPLGEGDVVGDVVACPWHNWEFNVKTGACINNPSAKVTAYEVTVEGTDIKVRL"
     gene            24230..24598
                     /locus_tag="PK589_11405"
     CDS             24230..24598
                     /locus_tag="PK589_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20254.1"
                     /translation="MADNQLTPKHIEIAETFVRLYVFLTQYIDRCEDEAARKEYPEAE
                     LQKHLGETRAKMVDILKVNPVVKGKVEKECERVLALGSKCLMGGTEKVAALDVLGAER
                     VILKNKTIALSDLLAVYRAL"
     gene            24604..24957
                     /locus_tag="PK589_11410"
     CDS             24604..24957
                     /locus_tag="PK589_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20255.1"
                     /translation="MPESAGEKHQLAGFDARERGFNRPVELVRTASGYQATLRYESTQ
                     VVTPDAPTTADALQHMIRILQEQGYTQLRSQQSYRDGVYLGSQELWIEYPDRQPEPEV
                     GLLGLMKKWLGRSPG"
     gene            25085..25528
                     /locus_tag="PK589_11415"
     CDS             25085..25528
                     /locus_tag="PK589_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNJ20256.1"
                     /translation="MIWLYLLPFTFLVLLHSNPVEAKIYSCEGPNGGTMLTDQPKGKR
                     GCTVVKTPSPSPPGGFTPPVEEAPAAPPDLQPNTFLPSQPVSPIHQPNPRPLDPIDKL
                     QPPTGSQAGGEKAAPEAQRCSPRVNPLNPFAGMNCSPTADETKKP"
     gene            25714..26895
                     /locus_tag="PK589_11420"
     CDS             25714..26895
                     /locus_tag="PK589_11420"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20257.1"
                     /translation="MHRDIVCLQIPSFEIVLARTHEASLRHRPVALAPVHTPRGLVRE
                     VSQEALDDGVLPGMSVELARQLCPGLRVIPPDSSRTQAAHRELQQAIMPFAPVWESIQ
                     PGSLFLDLTGTQRLFGRPIDTAARLERELTVKWGWHSQIGVAMNKLVSHLAATTLEKP
                     PQMRSIYSGSEQAFLAPLPATLLPGLNRLQASHILRRLDDLNLRTLGSIADVSLVQLE
                     AVLGTAAVVLHDWAMGIDHSPVRPSVEQPQIEQSLTLNPDEIDDHLILGRLSHLLERI
                     CARLRQQRRMCRRLSLALRHSDHAERMAQHILPYGTYWEADLQPVLAQLFARCFRRRV
                     RIQRIIVRADCLEPLAEQLRLFDESTIAVQPASHRISLVLDAIRAKFGERAIAWGKTL
                     P"
     gene            26892..29987
                     /locus_tag="PK589_11425"
     CDS             26892..29987
                     /locus_tag="PK589_11425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNJ20258.1"
                     /translation="MTTTPFVHLHVHSDYSPMRGVSSLETLCRSAKRQGLGALAVTDT
                     NGLYGAIRFIEEARHEGLRPILGAELTTDTHRALLWAKTPGGYANLCRLLSARHCEPS
                     FAFIEAVARYRDGLIIATDDQAALEAWLADDSQDLYVELTPGPAMQKTLEMSRRIGVP
                     SVATNRVYFARADDFATHRLLRAIALNQTFSRLPQAACCNPEQWLVPPERMAAAFPHV
                     PEALANSVRIAEACYSDWHFGDTIFPAFRELTDNAAFLTLKDDTYAGAQERYGLLSEE
                     VRARIEKELAVIRAKRFAHYFLIVKEILTQEPLTCGRGSVAASIVSYCLKITHVDPLK
                     HHLFFERFLNPGRKDPPDIDIDFPWDKRDGVLNWVFRQYGERQAAMVANQNSLGFRAA
                     IRETAKVYGMPADEIGTMSARVSRQQAILDFSTPPTPEQWLNRLSRTLRLKAPWPEIL
                     EQALRAQNHFRHLSMHCGGVVIVPDEIRRYVPVEFTAKGLPVIQWEKDQAEESGLVKI
                     DLLGNRSLAVIRDALDAIARHTGRTIEYASWDPLHDHATQDLIRRGDTIGCFYIESPA
                     TRLLLRKLWSGMPPDRLAVADVFEYLVMVSSLVRPAAITFVHDFVRRAHGHPYEPLHP
                     LMDNMLQETHGIMVYQEDVTKVAMALADFPLEDADQLRKVISKKHKSRQLRDYYQQFC
                     RGAAKNGAAPDTIDKIWTMIMSFAGYSFCKPHSASYAQVSFKSAYLRAHYPAEFIASV
                     ISNQGGFYSAFAYVSEARRMGLAILPPDVNASDWAYRGEGDRLRIGLMQVKTLQKELG
                     ERIVENRTEAGPYRSFEDFRRRVNLPPTQARALIRAGCCDSLAGELTRPALLWRLYAE
                     EQRHAGPLPIPEDYTDTHKLTHEIESLGFLTNRHPLTLYRHSIERLRPVPAAHMHRHV
                     GQRITMVGWLVTEKPVETRRGQAMEFVTLEDTTALYDATLFPNVYRRCHQFLAPNRAY
                     LVRGLVEAVFGVVTLTVTELQPLDAEPVREPAWNGARDAWYGESCDDRSDQPPPFSLP
                     N"
     gene            29939..30577
                     /locus_tag="PK589_11430"
     CDS             29939..30577
                     /locus_tag="PK589_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HNJ20259.1"
                     /translation="MRRSLRSAASFLPSKLTLPTLRNAAASCTGCDLYKTATQTVFGE
                     GPARATLLLIGEQPGDQEDVAGHPFVGPAGRVLDKALEEAGIARSDVYVTNAVKHFKW
                     EPQGTRRKHKKPSAAEVAACRPWLEAEIQVLEPRAILCLGVTAAQSVFGKSVRVNELR
                     GRPLTAPLGKTVFVTTHPSAILRGPDPAQREEDYRHFVGDLRLIAEYLQQRR"
     gene            30714..31088
                     /locus_tag="PK589_11435"
     CDS             30714..31088
                     /locus_tag="PK589_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20260.1"
                     /translation="MAVFSSGLFSRRTQDLRAGLRHAFALRSDQPAFTTEDLALLERI
                     ADAIVTRRMAAPATLFLESMGPMNFLGSQALHFLAPLVECVFSSSELAQIARLLERRD
                     SLQRLTALIEAKAAASKGAPAR"
     gene            31085..32938
                     /locus_tag="PK589_11440"
     CDS             31085..32938
                     /locus_tag="PK589_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNJ20261.1"
                     /translation="MTAAHQSPATSNPAQPLNVIVATDCGSTTTKAILIEKVGNEYRQ
                     TYRGEAPTTVEAPFEDVTRGVLNAIAEIEELSGRTILDGDRIITPNQAAQGDPQRGVD
                     IYVSTSSAGGGLQMMVTGVVQNMTGESAQRAALGAGAIVIDVLASNDGRLPYEKIERI
                     RTMRPDMILMSGGTDGGAVTHVVEMAEYIAAAEPRPRFGSTYRLPLVYAGNKDAQPQV
                     KTILGEKTALEFADNIRPVLERENLAPARNKIHDLFLEHVMQQAPGYKKLIEMAGAPI
                     MPTPAAVGVIMETIAKREGINLIGVDIGGATTDVFSVFGGLFNRTVSANLGMSYSISN
                     VLAEAGLENIMRWVPFTIDEQTLRNRIKNKMVRPTTIPQSLDELQIEHAIAREALRLA
                     LIHHKSLATGLKGVQQERTISDVFEQRTSGKTLIDLLKLDLIVGSGGILSHAPRRIQS
                     MLMMVDAYEPLGVTTLSVDSIFMMPHLGVLSTVNEQAATDVFVRDCMVYLGTCIAPIG
                     QGKDGERCADYEVALPDGRIEKGTLTFGDLKLFSLTRDQQATVTLQPSKQVDLGEGPG
                     QSFTRTVKGGVVGLMLDGRGRPLQLPTEQAARVALLTRWYQAVGLYPTGSS"
     gene            33134..34261
                     /locus_tag="PK589_11445"
     CDS             33134..34261
                     /locus_tag="PK589_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20262.1"
                     /translation="MAHSYTPGLTVTERTTVRRRRILPLPGAVLVQVGDAVRADTAVA
                     RAELPGKVVPLNLANQLGIAPDEINDYLVKKEKDSVQKDDVLAENKPFIKWFKTEIRS
                     PITGKVESVSTITGQILLREPPRVLELRGYIDGVVAEVHPEQGVTVESTCSLVQGIFG
                     IGGETSGELVMGVKAPDQPLLPEQLNPSMKGKVVVGGAFLSAATMTRAKELGIVGLVV
                     GGIHDKDLRALLGYDLGVAITGTEQVGFTLILTEGFGTIPMAPKTFALLSAHAGQKAS
                     ISGATQIRAGVIRPEIIIPHSGPAAVTKLPKAEREGIQLGDPVRIIRDPLFGKIGAVS
                     GLPSELRAIPTESEVRVLEVRFPDGTTTVVPRANIEVIEGA"
     gene            34267..35505
                     /locus_tag="PK589_11450"
     CDS             34267..35505
                     /locus_tag="PK589_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20263.1"
                     /translation="MLRRILILVFVISLPAIMPTLAPAQAMPQQLRVIDTPSDGGGSL
                     TVLWAPSATDSATTKYQILVHEGGLPQDPTAWKVVAEFPANTRYVREGKSAWWTRSGD
                     PNDHLFLIKNGKGLEVKSGQPYAVTVASVNGKERLIAPPVIASAEPNWMNWNQVNNLV
                     LALMFGAIVFYAINLAKRKDIFLRRIPGLDAVDEAIGRATELGKPVLYMTGAHDMNDP
                     STIAAAVILGRVAKKAASYETELLVPHREPITMAVCQEITKQAYMEAGKPDLFKDDAN
                     FFITSDQFSYTAAVDGIMLRRKPAANFFMGSYFAESLLLTETGASTGAIQIAGTDSDH
                     QLPFFVTTCDYTLIGEELYAASAYLSKEPIQIGTLRGQDLGKAFILSVIGIGTLLATV
                     AVLTGNGWAQPLLDLFKDLK"
     gene            35559..36152
                     /locus_tag="PK589_11455"
     CDS             35559..36152
                     /locus_tag="PK589_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20264.1"
                     /translation="MAAQYYVPHPASEQLLTTVTKWLQIIGGFALVLGITSLFHVHAA
                     KIRRKEAGWAYSLVLYVGMLGTIIVGLWNNGKESIDGALTSFGWVYSYTLVPLQGTMF
                     AILAFFIASAAYRSFRARSREAAVLLVAAVIVMMGRVPLGEYILPLSGDLSSWILNVL
                     NASVRRAILIGVSLGAVALSFKIIFGVERSYLGGGKE"
     gene            36149..36994
                     /locus_tag="PK589_11460"
     CDS             36149..36994
                     /locus_tag="PK589_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20265.1"
                     /translation="MTFAERMLRIDRRIIFLVIGLCTLVPLLFPVGLPIKISSEVRGV
                     YDYIEGLPEGSVFLLSLDFDPASKPELYPQAIAILRHAFKKNLRVVAMTLWVSGTGLA
                     DQILTQTAKEAGKENGKDYVFLGWSPGGSAVILNMGQDLYNAFPADYGGKATKGLPVL
                     AGVQNLRDVTYAVSLGAGNPGVEAWYVFGKDKYKFELGGGCTGVIAPGLYPLLRSGQI
                     NGLIGGLRGAAEYETLIGQKGKAVAGMDAQSATHLAIIVLVAICNIFYFSLRRQQRRQ
                     DGMGS"
     gene            37000..37755
                     /locus_tag="PK589_11465"
     CDS             37000..37755
                     /locus_tag="PK589_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20266.1"
                     /translation="MELSFGVILGAWIATGLTLFILSFLYEDNPLFKLAEHLYVGVSL
                     GYTIVKTYDTVIMTLIVRPILDKGEWSLLIPVGIGMLMLTRYVPKAAWLSRYAFAFIV
                     GVGAGLAIPRTISSFILKQIEDTVRPLLGIAPGGGVTFDYSLLNPASHLNGIIILIGV
                     VSVLFYFFFSVEHSGPGKAVARAGILFLMISFGAAFGYTVMARMSLLIGRLTDLIEFS
                     DASYGRPTLWLVLLTVATLIVLSRRGSTHPPNQ"
BASE COUNT         7968 a        11774 c        11005 g         7122 t
ORIGIN      
        1 tacagacccc atcaccataa ggaggcggga tgttcgaacg gttcacggac aagggtcgca
       61 agatcatcat cctggcacgt gaagaagccg agcgccatca gaacgattac ctgggcaccg
      121 agcacctggt gttggccatc cttcgcgaga cggatgggat tgcgctgatg atcctgaaaa
      181 aaatgggcct ctcgaccgaa cagattcgcc tcgaaatcga gcgcaacctg ccgggcggcg
      241 gcacgacgat gaccttcggg gaaattcctt tcagcccgcg ggtcaagaaa gtcatcgagt
      301 acggggtgga agaagcccga ttactcggtc acaaccatat cggcagcgag catctgttgc
      361 tcggcctcct gcgtgaagag gaaggcatcg gcggaaagat tctgagaagt cttggcgcca
      421 acctcctgac tgcccggcag ctcacggtga ccttcctgcg aaagtctgct cctcgcgaga
      481 gagaccgtaa gagcaacacc ccggcactgg acgaattcgg acgcgatctc acccaattgg
      541 cccaagaggg tcaattggat cctgtcatcg gacgcgcaga tgagatcgag cgggtgctgc
      601 agatcctcag ccgccgctcc aagaataatc ccgtgctgat cggcgaatcc ggcgtcggca
      661 agacagccat cgtcgaggga ctcgcgcagc gtatcattgc ctccgaggtt cccgacaatc
      721 tcctgtcccg tcgcgttatt gctcttgatt taggctcgct cgtcgccggc acgaaatacc
      781 gcggccaatt tgaagagcgg ttgaaagtgg tgatgaagga gatcgtgcag gcgggcaata
      841 tcattatttt cattgatgaa ttgcacacgc tggtcggcgc aggtgctgct gaaggctcga
      901 tcgacgcctc caacatgctc aagccggctc tgtcgcgcgg tgaaattcag tgcatcggcg
      961 ccacgacgct ggacgaatat cgcaaacaca ttgagaagga cggggccctc aagcgccgct
     1021 tccaacctat tcatgtgcaa ccgccgagca cggatgaaac ggtccgcatc attcagggtt
     1081 tgcgcgatcg gtacgaagaa catcatggcg tcgagatcac cgaggacgcg atcgtggaag
     1141 ccgtcaagtt ggcagaccgg tatatcactg accgattctt gccagacaag gccatcgatc
     1201 tgatcgatga aaccggctct cgcgccaaat tgcaaaccta cgcgcttccg actgaactca
     1261 aagcgctgga acaggagctc aagaagatct cgcgtgaaaa ggaactggct atctcgatgc
     1321 agaatttcga ggaagcggtt cgtcatcggg aagaagaaga gcgcctgcgg aaactgctcg
     1381 acgaatccaa gcgcgaatgg aaaaagaacc aggaaaagca caaaccgacc atcggcaagg
     1441 aagaagtcgc ctatgtcgtg tccaaaatga ccggcattcc gctcttcaag ctggaagagg
     1501 aagaatccaa caaactgctc cgtatggaag aattcctcca caagcgggtc atcgggcaga
     1561 acgaagccat ctcggcggtc gcacgagcca ttcgccgatc ccgggccgga ctcaaggaag
     1621 cgaagaagcc tatcggctcc tttatcttcc tcggaccgac cggtgtcgga aaaaccgaac
     1681 tggcgcgaac cctggcggag ttcctcttca atagtgaaga tgcgctcatc cggatcgata
     1741 tgtccgaata ccaggagaaa ttcaccagtt cgcggctctt cggagcccct cccggttatg
     1801 tcggatatga agaaggcggc cagctgaccg agaaagttcg tcggcgccct tattccgtgg
     1861 tgctgttcga cgaaatcgaa aaagcccacc cggacgtgtt caacgtcctg ttgcaggtgt
     1921 tggacgacgg cgtcctcacc gacagcctgg gcagaaaagt cgacttcaag aacacggtcg
     1981 tcatcatgac gtccaacatc ggtacgaagt tgattcaaaa gggcgtctcg ctcggtttcc
     2041 agaacgacga aaagagcggt caggcgttac gcaagaagga tgaagtgatg ggcgagctcc
     2101 gccgatcctt cagcccggaa ttcctgaatc gtatcgacga gatcgtggtt ttccactcgc
     2161 tcgagaagga acacctcatc catatcctgg atattctgct gcacgagctg aatctccgcc
     2221 tcagcgacaa gagcgtcaac atcgaggtgg acgacgaagt caaacagtgg ctgatcaagg
     2281 aagggtatga accgctgtac ggtgcgcgcc ccatgcgccg catcattcag cgagcgatcg
     2341 gcgacccgtt gtccgaggag ctcatcaagg gccgcttcaa ggataaccgg aagatcaagg
     2401 tcgttctgcg cgacggcgcg ccggccttta ttgaacagga agccatggcg ggcgtgtaga
     2461 acacgacgcg cttatcacga taggatcgat tggtacttga gatcctgtac gctacccggc
     2521 aacccccgcg gtccccaacc ggatccgggg gttgtcgctt ttcacggcgg agacaattgt
     2581 tgaattcatc caaccccaaa ccgtctctcg cccgaagggt gacagccacg tgtccacacc
     2641 ccctggcgcg gtcctctgaa gcaaacgaac ggtcagctct tccaacgcgg cggtatcaat
     2701 ctgccctatg actcacgcag cttcggcaac aatcctcggt cccattctcg gcatcgaaac
     2761 ctcctgtgac gaaactgccg ccgccgttct cgatggagag ggacacgtcc tttccaacat
     2821 catttcatct caacaagccg tgcatgagcg attcggcgga gtggtgccgg aactcgcctc
     2881 gcgagctcat atccagaaca tcgaccaggt gacaggccgg gcactcgagg aagccggtct
     2941 cggatggcgc gatctgcggg ccattgccgt cactcagggg ccagggctgg caggcgccct
     3001 tctggtcggc ctcagttatg cgaagtccac agcctatgcc ttggatattc cgctggtcgg
     3061 cgtcagtcac ctcgaggggc atattgcgtc ggcctggatc aatcgtccgg catttccaag
     3121 agactgcgta gtcctcattg cgtcgggggg acatacgcat ctgttccacg cggaagagaa
     3181 gggtggattt catctcatgg gacggaccat cgatgatgcg gcaggcgagg cctttgataa
     3241 gggggcgcag atgctgggtc tcgggtaccc cggcggaccg ctcatcgatg ccgcggcgcg
     3301 gaaaggccgt cccacagccg tgcgatttcc gcgctctcgc gtgagaaccg gtcagttcga
     3361 ttttagtttc agcggactca aaaccgcgct gctttatcat ttacaaggca tgagcctgga
     3421 ggccattgcc gagcaacaga ccgacctggc tgcggcctac caggaggcaa tcgtggatgt
     3481 cctggttcgc caggccttcg ccgccgtaga gcgggcaggg gtgtccgccc ttgccgtcgt
     3541 gggcggtgtc tccgcaaatt cgcggcttcg agcccgattg gccgagcgag ccgtcaagga
     3601 aggcattgaa ttaagcctgc cggccctctc atactgcact gataatgcgg cgatgattgc
     3661 cgctgcagga caacgagcgc tcctccaggg gaatgtcgcg tcattcgact gcgaagccat
     3721 gccggactgt gcgctgccgg cctggtcggg gagcggggtc gtccggcgca cctcctaatg
     3781 ttctcatcgc aaggagtccc gtcatcgcca cgattcacgg acatgtcagc ggcctgaaag
     3841 ccagccacct tgccgcgctg gaacgattgt atcgccgccg catccctgcc tctgtagtcc
     3901 tcactgccga attggctcgt acaacaaccc agctgtcacg tgaaattcgc cggcccattg
     3961 gactcctgat cacgcgccgt ggagccattg agcaagtcct ggtgggggca ggctgtgcac
     4021 cgacgttcga gtcgctggcc aaattcaggg tcggatcaca ttcattgcgt ggcctcagac
     4081 tgatccgcac ccatcttcat gacgaaccgc tgagccagga tgatctcaca cacttagcac
     4141 tcctgcgatt ggatctcatc ggagcgttgg gtgtgaccga gacgggcgaa cccggcttgc
     4201 tgcatctcgc gcacctgctt ccgcccaatc agcaaggcga ggtgtgccgt ctcctgaagc
     4261 cggtgccatt ccatgatctg gaactgcaac tggatacctt cctccatacg cttgaaagcg
     4321 acctccagcg atcggatacg cagcacaccg tcaccggagg ccaggaatca gccattttgg
     4381 tgagcgccgc gccgaaaggc catgccgaac aagacgagca tttagaagag ttgacggaac
     4441 tcgctgaatc agccggcgta cgagtcctgg atcgtattgc gcaacgcacc catgaggggt
     4501 atgagcggta tttactcggc aagggcaaat tgaaagacgt cgtcatgcgg tcgctgcaga
     4561 agggcgccga cctcatcatc ttcgatcagg acctggcgcc ggcacaggcc agagcgatct
     4621 ccgaagtgac cgacctgaaa gtcatcgatc gcacccagct cattctggat atttttgccc
     4681 ggcgcgcgca cacgcgagaa ggcaaagtgc aagtcgagtt ggcccagctc cggtacctgt
     4741 tgccgcggct ctcagggcat ggtacctcat tgtcacggct cggcggcgga atcggcacac
     4801 gcggtcccgg cgaaaccaaa ctggaaaccg atcgccggcg catccgcgac cgtatcgccc
     4861 atctcgagcg cgagatcgac cacgtggctc gccatcagga tcagcggcgc gcgagacgtg
     4921 tccggcaggg gctcccgatt ctctccatcg tcgggtatac gaacgccggc aaatcgaccc
     4981 tgctcaacgc cttgacccag agtcagattt cagcgcagga ccgcctgttc gaaacgctgg
     5041 ataccaccag ccgccgcctg cgttttccgc atgatcgcga agtcattgtg accgatacgg
     5101 tggggtttat ccgggatctt cccaaagatc tggtcggcgc cttccgcacc acattggatg
     5161 agctgcgcga tgccgacatg ctactccacg tcctcgatgc ctccgcatcg aatctcgacc
     5221 agcagattgc ggcagttgat gccgtgctgc aatcgctcca attggacacg attccacgcg
     5281 tcatggtgtt aaacaaatgt gatcgactct caccgcgtga ggctgatgtc ctcagccaac
     5341 ggtatcatgc cattgctatc tcggcactgg accgggaaac gctccggcct ttgatcgcac
     5401 acctcgaagc attgttgccg gccgttccca gccccccttc gcacgaggca gataccgagc
     5461 tgccaccgca agagcgggcc cttgcatctc actcgtgacc actgcacaat tggccgccac
     5521 aacccgcgca tcatgaaagc tgccaccgca catcacgtgt ccgctcagcc cgacaaacgc
     5581 cgcttggcgc gaatgctccg cgcgttacgg gccaccgagc ctgcccggaa agtcgagttg
     5641 gactatcgca caccgtggga gttgctggtc gccactattc tctcggctca atgcaccgac
     5701 cagcgcgtga accaggtcac cccggcgctc tttcgccggt atcgacgtcc gcaagactat
     5761 gccgctgctg acgctgcgga gttagaagcg ctgattcggc cgacgggatt ttttaagacg
     5821 aaggccaaga acctcatcca ctgtgcaaaa gccgtggtcg aaatatttca tggccaggtt
     5881 ccggaaacca tggaagcgct cacgggactc cccggagtcg ggcgtaaaac cgcgaacgtt
     5941 ctgctgggga atgcgttcga taagccggcg atcgtggtcg atactcacgt gaaacgagtg
     6001 gcggggcggt tgcacctcac tcgccataca gatcccgaaa aagtcgaaat ggacctccag
     6061 cagttgctgc ccgcagcgga atggacggaa ggatcgcagc gtctccttct gcatgggcga
     6121 tacgtctgcc tggctcgcac cccgaaatgc ggaaactgcg ccatctacac cgattgtcat
     6181 tgggaaggga aactcacacg atgatccgaa gtatgaccgg atatgggaaa aaggatggca
     6241 cgtcgcaaca ggccaccgtc accgtcgaga ttcgctcggt caaccaccgg tttctggaag
     6301 tggcggtcag ggttcctcga tccctggcgc agttggaaga tcccattcgc aaggccgttc
     6361 aacaacgctg cctccgcgga agagtcgatg tctccgtgtc agtccagacc gcggggggca
     6421 gcctgaaaac cgtccacatc gatcaggtac tggccaagca gtaccatacg gccctcaaaa
     6481 agctgcagaa gacgctcgga ttgcggggcg gggtggatct gtccatggtc gcggggtttc
     6541 gcgacatcgt ctcccttacc gatgaaccgg tggataccga ccatcttggc aagaccctgt
     6601 tgcgcgtcct cggcggagcc ctgaccgagc tggagaagat gcgcaagcgg gaaggtgagg
     6661 cgctgacgaa agacctgacc cttcacctgg acgcaatccg ggaggcacag tccatcgtgg
     6721 caaagaaggc gccggatctc gcgaaaatcg cctttgatcg catgaaagcg cggatcgaga
     6781 cgcttctcca aagcgaagtc ccggatccgg cccgtctcca gcaggaactg gcggtgtatg
     6841 ccgatcggtc ggacatctcg gaagaattgg tcagacttga gtcacatatg gtacagttcg
     6901 accagcagct ccgtagccag gagtccgtgg ggaaaacgct ggaatttctc ctgcaggaaa
     6961 tgggacgcga agtgaacacc atcggctcga aagccaacga tgcggagatt gccgcgctgg
     7021 tcgtccgcat gaaagccgaa ctggaaaaat tgcgcgaaca agttcaaaat gtggagtaac
     7081 gtcggcgcag cgccggcatt gaatcatcta tgagcactgc gcccgttcca tctcacgaca
     7141 aaccggctca agtacggcgg ggcattctct tcatcatttc cgccccgtca ggaagcggga
     7201 aaacgacgct gtgcaaacag atcacggcca atgtgccggg attgtggcat tccgtttcct
     7261 acacgacccg taagccgagg ccgggtgaaa tcgatgggca ggattaccat ttcctggacg
     7321 aagcgtcgtt ccggcagatg atcgatcgga acgagttcgt ggaatgggcc catgtctacg
     7381 gaaatctcta cggaacgcct cgcaaagagc tgacggaaaa aatggagcag ggcatcgacg
     7441 tcctgctcga aatcgatgtg caaggtgccc gttcggtgaa gaagaagttt gaagacggcg
     7501 tgtacatttt catcctgccg ccctcgttcg atacgttacg caccaggctc caaaaccggg
     7561 ccggagactc gccggaagaa attcaacggc gcctgcagaa ggccaaagaa gaagtctgga
     7621 gctaccgcga gtattactac atcgtccgca atgacgactt gaaacaatcg ctcaaggaac
     7681 tggaaagcat ctttctggcg gaacgcatca agaccaaacg cctgaacatg acctggctgg
     7741 aagaaaagtt tattctcgac aaagagggca agcaggggaa ttcctcctcc gcccccgcat
     7801 caaaggagca catgcatcat gggtgaaatg ttatcgttgt taccggaata tgccaccggc
     7861 gaattcgact cgcgccaccg gctggtgatc gtcgcggctc aacgcgccaa gcaattggtc
     7921 caaggggccc gcacgaccgc atcctccaag ttcacgaaag aaaccagtat cgcactggat
     7981 gaggtgttgc ggcacaacgt gaagtttttg gtcggcaaag aagcccgcga tgcgatcaag
     8041 gaatccaagc gcgtgaagga aggcgagact gaacgcctag ccatgatgac cggagaagac
     8101 gccaaggaga tcaagaagga actcagcgtc tacgttgacg ataccgccaa gccggtcgcc
     8161 gccccggagg ccgaggagta gtgtcccttc gtgcacacca ccggcccttc actttccggt
     8221 gtccgtcttg tgctcgccgt cacggggagc attgctgcct ataaagcggt cagtctgttg
     8281 cgcctgctcc ggcgcgaagc cgcgacagtc aacgtggtga tgacggccgg cgcctgccgg
     8341 ttcgtcaccc cgctgacatt tgaagtattg tccggcacac atgtggcgac cgacctcttc
     8401 gaagcgcacc aggagatgct tcacctgtct ctcccggaac aggcgcaggc catcgtcatt
     8461 gcgccggcca ccgccaattg ccttgccaaa gccgccctcg gactcgccga cgacctcctg
     8521 tccaccatgc tcctcacaac tcaatgtccg gtgattttcg cgccggctat ggacggggac
     8581 atgtggcagc atcccaccgt cgtggagcat gtcgcgacct tgcgcgcacg cggagccatc
     8641 atcgtggaac ccgaggatgg tccgctggca tccggccgac aggggcaggg caggttagcc
     8701 gatgaggaac gaatcctggc agcggttcac cacgccgttc atccgcgccg agactggtcc
     8761 ggccgcaagg tgctcatctc agccggcccg acccaggagg ccatcgatcc cgtacgattt
     8821 ctctccaacc ggtcatcagg aaaaatggga tacgccctgg ccgaagccgc cagggctcgt
     8881 ggcgcggacg tcgtgctcgt ctcaggccct acctcgatcc ccgtaccagc cggggtggaa
     8941 tactgtccgg tgatcacagc tgaagagatg cataaggcgc tgacaaccca attggattgg
     9001 agcgatacgg tgatcatggc tgccgctgtc gcagattttc gtcccgcccg gccatccgca
     9061 cacaagatca agaagcggcg cggtccggtc acgcatctgg atctcgaagc gaccgacgat
     9121 attctttgcg aattacgtga tcgccggacg agtcaggtgc tggtcggatt tgccgcagaa
     9181 actgatgatc tccttgccca cgccaaagaa aagctgcatg ctaaaggcct tgatctgctg
     9241 gtcgcgaatg acgtgaccgc gccagggtcg gggttcggat cggataccaa ccgggtctgg
     9301 ttgctggccc gtgaagccga tcccgaggaa ctcccgctgt tatccaagcg cgacgtcgcg
     9361 gaccggatac tggatcgaat cctgacagtc catacaggcc ggtaactcga gagcaaccgg
     9421 tcgcagccgc atcgcaagga gttacctgtg tcgtcaccac tgcgcattcc tcccgccatc
     9481 gccgcagaga tcgatcgact ggccacagcc gtggcgaagg atccacgatc gaaagacttc
     9541 ctgccgctgg cagatgaata catcaaggcg ggcatgtggc aggaagccgc ggccgtgctc
     9601 gaagatggcc tgaaggtcta tccaggattc gtgaccgcca tggcggcctt gggcagggtc
     9661 tacgatcaac tgggacaagc ggaaaaagcc aaggtcattc tcgaggaggt cgtgaaacaa
     9721 cgccccgaca atcttcgggc acaccgcatt ctcgccaagc tctatcatgc ggagggacag
     9781 gccgacttgg cactccgttc ctgtacagcc atcctcaacg ccaatccctt cgatgaggag
     9841 gcgttggcag tcaaacgcag tgtcaccggc gcgccggacg agccgccgcc atcgaaacgc
     9901 gagaagaagc gcagcctcgc ggaagcaccg gtggaaaaga tcacggcagc ggtcccacct
     9961 gcgccggctc cggttgtcct tgtatcagag tccgcggcgc ctaccgtgtc gatgcccgat
    10021 ccgacgcctc cccagagccc cccatctccc gctgttcaac atgcagccgc aatcgcgcgc
    10081 ctcgaagcct ggctcagcac catccaggct aaacgccacc ccgcagcccg gtgactgaca
    10141 cactcaccgg gactctttca gcgtttgacc cacccctgac agcctgttag aatgccgccg
    10201 ctgctggcat ccctatccat tgcagcctac tgacaaccgg ggcgcgcgac cgacagatgc
    10261 tgcggctact tgtgcttcat ggtcccaatc tcaatctcct ggggacgagg gaaccctccg
    10321 tgtatgggca gatgtcatta tccgacatcg ataagtccat tacccgccgg agcggtgaac
    10381 tcgggattgc ggtgcagacc aagcagtcca atagtgaggg ggagttggtc acctggatcc
    10441 agaatgccag gggacatttc gacggaatca tcatcaatcc ggccgcatac acgcatacca
    10501 gcattgcgat ccgtgatgcg attgccgccg tcgccctgcc gacggtggaa gtccacctct
    10561 cgaacatcca tcaacgggag gagtttcgcc atcattcctt cattgccggc gttgccatcg
    10621 gacaaatcgc cggtttcggc ccgaccggat acctgctggc gctggaggca ttgaccgtac
    10681 atctgggtgc caaacgctca tcgtcacgca cccgagcaga cgtgagaaag aaacccgcgg
    10741 cctcgcgccg ttgaacttgt tgcactaagg agagttgttg tgatttcgac cgcagagttt
    10801 cggaacggca gcccgttaat ggtagaggga caaccctttt atattgtgga attccaacac
    10861 gtgaaacccg gcaagggtgg agctttcgtc cggacgaagc tgaaaagcta tctctcgggc
    10921 aatgtcctcg accggacctt ccgttccggc gaaaaattcg acacccccga gattgaagag
    10981 tgcgatatgc agttcctcta tgccacgaac gattcctata cgtttatgga tacggagaca
    11041 tacgagcagt cgacttacga aaagagtcag ctcggcagca acgccgacct gctcaaagag
    11101 aacatgatcg ccaagatcct gatctatgaa cacaagccga ttacggtcgt gctgccgaac
    11161 ttcatcgaac tgaaagtcgt cgatggcgaa ccgggcgtgc gcggcgatac ggcctccggg
    11221 ggcagcaaac cggtcattgt ggaaacaggc gccacaatca aggtgccgct gtatctggaa
    11281 atcggggaag tcattcgcat cgacaccaga acccgtgaat ttgtggagcg cgtgcgttga
    11341 gcccatcacg taagaagacc ggaaaaagcc ggactgccgc taagcccatt gtgctgccga
    11401 gcgcctttgc gccacgaacc tctgcggccg accagatgat cttaaatccc gaccaggccg
    11461 cgcagattca gcaactggcg gatctgctga aacgcaacca tctgaccgaa ctggaaatcg
    11521 agcgtagcgg gatgcgcatc cggattcggc atgagccggc ggtacgcgcc acgacgacgc
    11581 ataccgtgga ggccgtccac acccaatcgg cgacatccgg cacggcaccc gcagcgcaag
    11641 ctcgtccctc ggcagagaca gacggccagg tgactattac gtcaccgatc gtcggcacct
    11701 tctaccgctc gccttcgcca gacgcagacc cgtatgtgga agaaggggac tacgtgaaga
    11761 aggggcaggt attgtgcatc gttgaagcca tgaagctcat gaacgaaatt gagtccgagg
    11821 ccgacgggcg catcaccaaa attctggcgg aaagcaccaa acccgtcgaa tatggacagc
    11881 cactcttctt gatagatccc agcgccacgc cctagccgca gaggtcatgg ggattcgtga
    11941 cgagacccgt cctgaacgca tcaacctcat gacgtctctt atctgcgacc gcctcacgcc
    12001 gaccggcaag ggccgatgtg agggctgaat gagcgcgacg gacaagggca gaatgacgca
    12061 gacgcacagc aagactcgca tcggagtatc tcgtgtttaa aaaagtattg gttgccaatc
    12121 gcggagagat cgcccttcgg gtcatccggg cttgtaagga gctcggcatc aaaaccgtgg
    12181 cgattcattc cgaggctgat gccgcaagcc tgcacgtacg ggcggcggac gaacatgtct
    12241 gcgtgggtcc tcctgaggcc gccctcagct atcgcaacat tccgaacgtc ctgagcgccg
    12301 ccgaggtgac cggtgccgac gccattcatc ccggttatgg atttctgtcc gagaacgcgc
    12361 actttgccga agtctgcgag tccatcggcg taaaattcat cggcccgacg tcagaaaaca
    12421 tcgccttgat gggcgacaag tccaaagcac gcgaagtggt cgccaagaaa ggcctcccgg
    12481 tcacgcccgg gagcccgggc gagctccgga gtgagcagga tgcgcaggag gcggccaaaa
    12541 acatcggctt ccctgtgatc atcaaggcct cagccggagg cggcggccgc ggcatgcggg
    12601 tggtgaacaa gccggaagaa ttgaccagag cctttcaagc ggcccaggcg gaggccaagt
    12661 ccaccttcgg ccacgacggc gtgtacctcg aacggtactt tctggaaccc cggcatatcg
    12721 aggtgcagat cgtcgcggac aaccgcggcc atgtcgtcca tctcggcgag cgtgattgct
    12781 caatccaacg gcgccaccag aaactcgtcg aagaaacgcc gtcaccggcc atcgacgaac
    12841 ggttgcgacg cgaaatcggc cggacggcag tcgaagccgt caaagccatc cgctactgca
    12901 atgtgggcac ggtggagttt ctgctcgaca aagaccggaa cttcttcttc atggaagtca
    12961 acacacgcat ccaggttgaa cacccgatca cggagatggt gaccgggatc gatctggtga
    13021 aggaacagat ccgcatcgcc tcgggactgc cgctctcgtt caagcagcct gacatcaagc
    13081 tgaacgggca tagcttcgaa tgccggatca atgccgaaga cccggagaag ttcaccccgt
    13141 gcccgggaca gatcaccaaa tactcagctc ccggtggctt gggcatccgc gtcgactcag
    13201 ccatggaacc gaacgcggtg gtggtgccgt attacgactc cctcatcgcg aagttgatta
    13261 cccacggacg cgaccgccag gaagcgatgg cccgtatgcg ccgggcgctg gacgagttcg
    13321 tgattgaagg aatcaagacc acgatcccgc tccataggaa gattttcaac gatccggatt
    13381 ttcaaaaggg gcatgtctcc acgacgttcc tcgaccgctt tctcgccggc caatccgcgt
    13441 aaccagccta cgataccagg gagcgcgcca cgtctcagtc cactcgacaa ctgtgcctca
    13501 agggactgta tcttatcctt gatcccaccg tcaggcctga acggccgctc acggacgtac
    13561 tgcgcgtggc cgcggcatcg ggaatacgcc tgtttcaata tcgggacaaa caggcttcga
    13621 tgaaagacgc ctatcggcag gcacttgctc tccgtcaggt ggcgacggaa gtgggggcga
    13681 cactgatcat caatgaccgg tgcgatctgg cattggcggt caaggcagat ggcgttcacc
    13741 tagggcagga tgatctcccg ctagccgacg cacgtcagat cctggggccg gaggccatca
    13801 tcgggctctc aacgcataat gcgaaacagg tcagagaagc ggtcgctctt cagccggact
    13861 acatcggctt tgggccgatc ttcgggacga ccaccaagac cgatcatgac ccggtcgtag
    13921 gccttgaagg attacgcgca gcacgcacac tcaccacctt gcccatcttc gccatcggcg
    13981 ggattacgtc tcagcatgtg acggacctca cggcggcagg ggcagacggg gtagcggtga
    14041 tctcggccat tctcaaggcc caggatcttg aggcggctat ccggagcttc cagccgccgc
    14101 cttccacaca agctcggcca agtcactgat cggccgactg ccggccacat tctctacaac
    14161 ctcgggccag gcgcaatctg cgcgagtctg ataggggagc gggccgatca agggcatcga
    14221 gactcgatcc cgtaacaatg ctaccgtcga tgctgcttgc tcctgttcaa cggagcctcg
    14281 tggtgccacc gtctcattca acaccaacgc aacgaccgga atgtttcggc gttcgagggc
    14341 ttccaatgtc agcagcgcgt ggttgattcc accaagaccg acccgtccga ctaacaggac
    14401 gggaagacgg agacggctga tcaggtcagc catagcccaa tcactgcccg tgggcacgag
    14461 gaggccgccg gctccttcaa cgaccacgca tgggtaacgc gcagccagtt gctcataccg
    14521 tccaacgatg tccccgagct cgatgacccg cccttgggca gcagcggcgg ataagggagc
    14581 caccggcgca gggaaacggt aaggtgaaac gaggtcgagc gaatccgtca cttgcgcagc
    14641 cgccatcaac cgagcggcgt cggtgcgatc cgacaagcct gcatccacgc cggtttcgac
    14701 cggtttcatg acgcccaccg aacggcccat ccgcacgagc ctgttgacca gcgccgcagc
    14761 cacgagcgtt ttgccgacac ccgtgtcggt acctgtcaca aacacgcccg tcgtccctat
    14821 catgcctatt ccgtctccac gatcgattag tagagaatcc ggctatccag attcacgatt
    14881 tgtcccgaca tgcccggaag ctgcgcgagg tgacaaatgg ttcggctcac atcctccaga
    14941 ttcatcagac ggcccagcac atgatcactc agctgttccg tggaaggaaa ggcatcaccc
    15001 gccagcgcgg tgggttgcca ccccgggcag acgagattca cgcggacatt gtgcggcccc
    15061 cattcgcgtg cagctgtctg cacaagcccg acaagcccgg ctttcgaagc cgcgtaggcg
    15121 gcttggccgc tcgctccatg caatccggca taggacccga ccacgatgat agatccgcca
    15181 ccctgcttga tcatcagcgg ggccacagcc gtgaggcacc gataggttcc cgtgagattc
    15241 gtatcgatga tgcgcaacca ctcgtcttcc gggcaacgga gcacgagatg gctggacgcg
    15301 acgccggcgt tgcacaccag cacgtcgagg cggtcttgcc gatccagcac agcatcgatc
    15361 atcgcctgaa tctccagacc tgaccgtata tccgcctggt agatggcgcc cgctcctccg
    15421 tctgtggaaa gctcgcgcaa cgtttcctcg gctgcttctt tgtggcgacc atagtgcacg
    15481 ccgacccaat agccggcgcg ggcaaaggcc aggcaaagag cgcggccgat accaccggac
    15541 cctccggtaa ccaggacggt tcggagttcg cgaggagatg acgcagatgt ctgtggttct
    15601 gattgcacgg cgagcgatta tgcgagccgc gcgggaagaa ggcaaggcag cagccagctt
    15661 gccgcggaag tcctggtatg gtaccgtgat tttttgactt ggaggacagc cggaatggtt
    15721 cagaacctgt ttcgctcaac cctgattgca gtaggcattc ttggatccgc ggccatcctc
    15781 gtggggcacg atacggcctc cgccaccgaa gcagggcagg ccatcgccca atccgcagac
    15841 tattttcccg acaccatcgg cacgcgctgg caatatcgtg gccagatttc tgaagggccg
    15901 ctgcaaacga tcgagaataa gtatttcagc aacgtctcct cggtcaccgg cacgcgtacc
    15961 ctgaagggcg gcctggtcgt caccgtgttc catgacacca atcccggtaa ccacggtccc
    16021 tccgacagct tctaccggcg cgatgccgcc ggcgtggtct actatggatc cgaaccgggc
    16081 acgccgctgg agaagcagtt gacgccctac cagatcatcc gctttccgat gaaagtggcg
    16141 caatcgtttc agcagttcaa tcggaccgac atcgattttg gcagcgatat cgacggggac
    16201 ggcaccaacg aaaaagtcga cgtcgaggga ctctcaacgg tgttgggcca ggaatcgatc
    16261 accgtgccag ccggcaccta tcctgatgcc gtgcgcgtgg aagcgaagat gaccctgaag
    16321 atcagactgt cctctgtgga tcggacggcg gtcggcacgg atgtaatgac ggcctggttc
    16381 gccaaaggcg tgggactggt caagtatatc gaacgccagg aattggccgc gctgaaggat
    16441 gaccgcggaa acatcaccga catcacggaa gagctggaag aggtcagcgt gaagtcggcg
    16501 ccggggctac agggtcggca cgaatccgcg ccggagggtt tgctcgctga cgacacgagc
    16561 aaccagaaat tgtttcaggt actcttcgcc tccggccttc gcgcctacgc ccgataaccg
    16621 gtgcccgccg aatggctgtc ggctgaccaa ggcgccggta atcgggcggt tgatgtacag
    16681 attcccgaca tcgaacgcct cccgggcacg cgcgatgtgc cggggactcc gcgaatagag
    16741 gccgccggtc agcgcgtagg ccgtgccatt ggcgtattcc aatgcctgct cgaacgaggc
    16801 gacgcgcatc acggccagga tcggaccgaa aatttcttcc tgcgccaacc ggtgcaccgg
    16861 ttcgatatcc gcgatcacta ccggcccgac cgaatatccg gggccttccg ccgaccgatc
    16921 caccagcacc cgaccttcac gccgcccgat ttcgagatag tccagaatgc gttgtttcgc
    16981 gcgcgcatcg atcaccggac ccacttgagt tgcgggatca tctgccgcac cgatgcggag
    17041 actcctcacc gcatcggcga ggcgttcgag aaacgcatcg tgaatcgagt cgacgacaat
    17101 cacgcgcgaa caggccgaac atttctgtcc agcatatccg ctaaacgatt gcaccacgcc
    17161 tgtcacggcc tcatcgagat ccgccgtctc atcnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnncgctaaa cgattgcacc acgcctgtca cggcctcatc gagatccgcc
    17341 gtctcatcaa cgatgaccgc gtttttcccg cccatctcgg cgatgacccg cttcacgaac
    17401 cgttgtcccg gttgctgaac tcccgcggcc tgcatgatgc ccaaccccac gtccttcgat
    17461 cccgtaaagg caatcgttct taccgctgcc gagaccacca attcacggcc gagctccggt
    17521 cctcccggaa tgtattgcaa cagccccttc ggcaccccgg catccaatag aatacgcgcc
    17581 agctggtacc ccatggccga ggagcgctca gacggcttga acaggactgg gttgcctgca
    17641 accaacgccg ccgccaccat gccggtcggg atcgccaggg gaaaattcca cggcgcgatg
    17701 accaccgtga cgccgcgcga gttccacaac acctcgttga gctcaccggg ataccggccc
    17761 aaacgaggcg ggggatcgag ccgtgccatt tcgccggcgt aatactccag aaaatcgatc
    17821 gcctccgcta catccgcatc ggcctcccgc cagggcttgc cttcttcgaa cacctcccag
    17881 gccgccaggt cccatcgttg atcccgcatc agtgaggccg ccttccggat caccgcgaca
    17941 cgatcagcaa ccggccgtct cgtccaggac tcctcagcag ccccggcaat ctttgtcagc
    18001 gccgcgacat cggcgggctg ataactttgc accaccgcca cgacctgctc ggggtgactg
    18061 ggattgcgtg tcgataggtc tggcccggtc ggaagatgcg ccgccagcgt tgacaggtcg
    18121 agacgttgcc ccaagtgttt gcggcgccgt tcgatcgcat cggccatcgc cgtacgcgcc
    18181 gacgctcgcg aaaaatcggc aaccggttca ttcacaaatt cggtcggtga tgtatagggg
    18241 gaagacttgt gctccaccgg cgatgaatag gccgggtgag acggggcgcc atcaggcggc
    18301 gataacaaca acgacagcgg ctgcgactcg acgtactctt tccgcaggaa ggattcgttc
    18361 gacgtgttct ctaacaagcg gcggacgaga taggccatgc ccggcaacag ttcgccgacc
    18421 ggcgtatata gccggaggcg ccgcccgcga tcactcatcg catgctggaa cggttccgcc
    18481 attccgaaga tcatctgata ttcccaagcg accggtggca ggcccagcgc ctccgcgacc
    18541 gcttcgatca cggccaggct gcggagattg tgcgtgccga aggccggcct gatcagatgc
    18601 gattgttcca gcaacagacg aaccagcgat tcataattca cgtcagtctg ggacttctgt
    18661 tcgaacaacg gcatcggcca accacgctga cgataccgga tggcgtcgga atcccagtag
    18721 gcacccttca ccaggcggat cgtgatcggc acctgccggc gtgtggtcca tgccagtaac
    18781 cgctgcacat cgtcataggt atcgatacgg tacgcctgca gcgcgatccc cgcgtgggcg
    18841 aaggtgcggt acggttcttc ctcgaagaga cgcatgaaga tggaaagaat taggtccttc
    18901 gtttccgcct gttccatatc aaaaatgatg gaggcgggga gcgtgcccgc gacattcagc
    18961 aacggacgca ggcgggccgc caccgcacga tagctgctct cgggatcgat cggatcgagt
    19021 tgtgagtaga gggccgaaat cttgatcgac aactggacac ggggaagcgg gccgaggtga
    19081 tcgtgctcga gcaacggcga gggggaccag gccgggcaga catcaccgag gatccgcagg
    19141 gctgccagac agcgatcccg ataggcatcc gcctcccgtt cgttcacact cgcttcgccc
    19201 agcagatcta cagaaaacgc ccgcccatcg tcccataatt ttcggagcgc cggcaccgcc
    19261 tgctcgatgg acgcgccggc aatgaagctg gtcgccatct gttccacctg gtgacggatg
    19321 gccttcccgc tgagcgccgc acccagcttc gtggacgcga gtgccttgag tccccactgc
    19381 gcgccgaaga gatgcccgct catgtcgcca aaatattctt ccgccagacg gaccacctgg
    19441 gcatcatcgc ggagcgacgg caacacatcg atgaagcgaa acagctgcgc cttgaaagcg
    19501 ggatctttca tggccaggtt gatcgccgcc tgcgaccacc aacgggaatc aaacaggccg
    19561 ggcgacaatc cgctggaacg gcgggcgaga tcttcaccga tggtacgaat acggggttca
    19621 agcagggcag ggtccagagt catgtggtcc tccaggacga cgtgaagagc tctttcctgc
    19681 agtatacacc gcgcctctgc acttgagaat ttggctgcgc gttcgagcga gaatcatccc
    19741 cgaggcggac aagaccacaa agaggaacgc catggagaat gccgggcaac ccctggctca
    19801 atctgcatgc gcgacggcga gaactgcgtt acccgcgggc cttcatccag aaccaacaca
    19861 tccctcccgg tcgatattcc gagagggcgg ttcgccgata gaccttcggg aggcttaatg
    19921 agagtggcca atcggccagc tgtcgaggaa gggctggaat ctccgtaatc tggtgcgtat
    19981 tttcaaaccg ctttcggatt acgcctgaaa tgccgggaat gagaaagtcg tggagttcga
    20041 ccaggatatc cgcacgagtg agggcaggaa tggcgaccgg atcaaggagc tcttgttcgg
    20101 cgccttcgac atcacagacg atcaaggttt ttcccggctg cgccattgcc gcctgcaacc
    20161 cagacggggt gcaagactcc cggatcgtca gacgcggagc caccccgttg atctccgcca
    20221 ttcgccccaa gatgacccga ctctcagcat cagcttcata ggccgtcacc cggctctgag
    20281 gcaggcaacg agcgaaaccg atcgcatagt acccttcacc ggcaccgatg ttgatcacgt
    20341 gagcgtaggg gtgtccggtt cgcagagcct cgacgagcgg atggagttcc tgttcatagg
    20401 tcccgagcag tttgggacag taggcgctgc tgtgaactcc gagtgcatgt tggatgtatc
    20461 gcatgcctgc aaacggcccc gagtgtacgc gggaaccggt tccccaggtc accacggttc
    20521 cgacgcagcg gtaaaagatc gaatcggcgt caaaaagacc gccctcccgg tggacacgct
    20581 ccaccgtcaa gatgcgctgc cgcgcagtat ccgacaggat tgtcttgagg actttacggg
    20641 tgactgttcc aagcatcgga agggtctcca gcggacatag agggtgacac gtacacgcca
    20701 ctctaggcgc attgcgggca cgggtcaatc ggccggaggc cgcctcgcgt cgtcatttct
    20761 gttcatgcgc ctgtacgtga acagctgaca gccgctccgt gcgcaggatt ataagtgatt
    20821 gtacagccgc ctccgaacgt gcagcactgg gaggggtgct gtcgaacaag agcgcgagcc
    20881 aggctggatc ggcgcgagga gtcttgcaaa ttctccgtgt cttgttagaa tccggctccg
    20941 accgcacgac cgcctgccca tcgccacagg tcggcacgcg gcttgtgcca gggccgccga
    21001 ctcctgcgtg atagatcctc gctcacctgc catcctccag tcgaacagaa aggaccacgt
    21061 gccatgcact ccacatccaa ggtgatttgc cagatgcctc ctatgccgat cgagaccacg
    21121 cacgagctga tcgaaataat tcttgcgaaa tgggaagggg cgatcgggga ggactacctg
    21181 ggatacaagg gacatgtgta ccgcatgttc aacttctgcc tggcgttaca tccatgcacc
    21241 gaggaggaga acacaaagtt agccatcgcc gcctgctttc acgatatggg tctttggcct
    21301 gatcgcacgc tcgattacct cccggcctca atttcccacg ccgcccaata cctgtccgat
    21361 tcagggcggg aagagtggtc tgaagaaatg gccctgatga tcggcatgca tcacaaggta
    21421 cgcgcctatc cagatcgccg ttaccctctt gtcgaagtgt ttcgaaaagg cgacctggtt
    21481 gatgtttcgt tgggcctatt gagttgcggg attccgcgat cgtacatcac agacgtgaag
    21541 aaccactttc ccaatcacgg ctttcacaca ttcctcctca agacaggcag agcctgggtg
    21601 tcgaggcatc ctttcaatcc cgcgcccttt atgcgctggt agcacagact gagtaggtca
    21661 ggtgaccttc acgcccacct ggcgtgcggc agccaaccgc gtttctcccc ttgaaaatct
    21721 gacggcggct ccgttacagt gcgccgtttg ccttcaccca ttctctaagt atggcggtgc
    21781 actgtggcga tgagttgcgc acaggttccg aaggagtacg atggccgact catccacaca
    21841 atccgtctgc tggggcaccg tcgccctctt cactgcgctc acgatcctga ccttcgtcgg
    21901 ggttcccctc ttcgcttatt actatgacta caccgtcctg gattggacgt tgctcgccat
    21961 actctatgtc gtcaccggca tggggatcac ggtgggctac catcggatga tcactcatcg
    22021 cagctttgag agccatctct gggtcaagat ctgcctgttg atcatggggg gatgggccgt
    22081 acagaattcc gctcttctct ggtgcgctga ccatatccgt caccatgccc atacggatga
    22141 agaagccgat ccgtacaatg cgagcaaagg attctggcac agccacgtgg gctggctgtt
    22201 catcaataca ccatatcgtg aagaccggtt tgccgcgaaa ttacgtaccg acccgatgat
    22261 tctctggcaa caccggtact actgggtgat cgtcgcctca ggactgttgc tgcccttcgc
    22321 ggccgggtat ctgaacggcg gactgatgag cgggctcggc tgtttcttac tcgccggcgt
    22381 gttgcgcacc gtcttggtgt tgaattccac cttcaccatc aactccctgt gccacatggt
    22441 cggcactcaa ccgcacggca cgcaggacag cagccgggac agctggctga tttcgttcat
    22501 cagcttcggc gagggctacc acaattatca ccatacctat tcgcacgact atcggaacgg
    22561 atcgaaatgg tacaatttcg atccgtccaa atggttgatc tatacgctct cgctggtagg
    22621 gctgacgtat aacctccatc gggaacgcac gtaaacggct ggtgaacaac gcccgccgct
    22681 gttgttctcg catcgctccg cccctcgacg tatttctgcg aataccccgc ggctcttcgc
    22741 tcgctgcgac cgcaccgggt agagtttttt gaccatcctc tcaacttatt cctcttacat
    22801 cgctgatctc ttcgacccaa ccgcctgctg cgccgactga cccatttctt ttcgccgcgg
    22861 cgatagctta atatgcgaga aacccacgta atccgatcgg accaacgggg tggaggaggc
    22921 atcactatgg cagaagaacc aggcggcgca ccgcatcaag acgaagcggc cgcccgaccc
    22981 aatattattc ccggagtcaa acatgtcgtc gccgtcagca gcggcaaggg cggcgtcggt
    23041 aaatccacgg tctccgtcaa tctcgcggtc gctcttgcgc tcaccggcgc caaggtcgga
    23101 ttgctggatg ccgatatcta cgggcccaac atcccgatga tgatgggggt cgagaagact
    23161 cctgagcaga aagacggcaa gattgcgccg gcggaaagcc acggcgtcaa gctgatctcg
    23221 atgggatttt tcgtgccgga agacacggcc gtcgtgtggc gaggcccgat ggtgcacacg
    23281 gccattcagc aactcttccg cgatgtgtta tggggcgagt tggattattt gttgatcgac
    23341 ctgcccccgg gaaccggaga cgcgcaactc accctgacgc agctcgtgcc gctgtcgggc
    23401 gccgtcacgg tgaccacgcc gcaggaagtg gcgttgcacg acgtgcgcaa aggcatgatg
    23461 atgtttcaga aggtgaacgt gccgctcctc ggcatcgtgg agaacatgag ctttttcgtc
    23521 tgcggccatt gcggagagcg gacggagatt ttctcccatg gcggcggaga gcgggcggcg
    23581 gagaagctgg gaattccctt tcttgggcgg gttcccatcg atcccgtcat tcgcgccgga
    23641 ggcgataccg gcaacccgat cgtggtcgcc aagcccgatt ccccgcaagc gcaggcgttt
    23701 cgcgacatcg cagccaaact ggccgcagcc ttgcaaacag ggggcgtgtc ggcgaccaag
    23761 agtcgcatcg aaagtttatt ggataaaatc aaacggccgg ctgcggatcg aaagccggac
    23821 taggacgagt gacgtactca gggaggatgt atgggcgagt tgatcagaat cgcaggaacc
    23881 gccgacgtga agccgggagc cggcatggtc gcggaggtga acggaaaagc cattgcggtc
    23941 ttcaatgtgg atggaacctt ttatgccatc gataacacct gcgtgcatcg cggcggcccg
    24001 ctgggcgaag gcgatgtggt aggggatgtc gtcgcctgcc catggcataa ctgggagttc
    24061 aacgtgaaga cgggtgcctg catcaataac ccgtcggcca aggtgacggc ctatgaagtc
    24121 acggtcgaag gcaccgatat caaagtccgg ctgtgatgca gggacgcgcc gcgcgcgacg
    24181 tcgccacagc ataatgatca tgtcgacacc ttagtgaatg gaggatccaa tggcggacaa
    24241 tcagctgacc ccgaaacaca tcgagatcgc cgaaaccttc gtgcggctct atgtctttct
    24301 gacccagtac atcgaccgct gtgaagatga agccgcgcgg aaggaatacc ctgaagcgga
    24361 actccaaaaa cacctggggg agactcgcgc caagatggtg gacatcctga aggtgaatcc
    24421 cgtcgtgaag ggcaaggttg aaaaggaatg tgaacgcgtc cttgcgttag ggagcaaatg
    24481 tctgatgggg ggaacggaaa aggtcgccgc attggatgtg ttgggtgcgg aacgcgtgat
    24541 cctcaagaat aagaccattg cactcagcga tctgctggcc gtctatcgcg ccttgtaaga
    24601 ccaatgccgg aatcggctgg agagaaacac caactcgccg ggttcgacgc cagggagcga
    24661 gggttcaatc gccccgtgga gttggtgcgg acggcctccg gctatcaggc gacgttgcgg
    24721 tacgaatcca cgcaggtggt cacgcccgac gcgccgacca cggctgacgc gctccagcac
    24781 atgatccgga tattgcagga gcagggttac acgcaattgc gcagccagca gagctatcgc
    24841 gatggcgttt atctcggctc gcaggaactc tggatcgaat atcccgatcg gcaaccggag
    24901 ccggaggtcg gcttgctggg cctgatgaag aaatggctgg gacgttcacc cggctgatgt
    24961 ccccctaagc ccgctcccgc agattgacag gttccggcct cgcgcctata gtcgtgcggg
    25021 atcatggcgc tccgcgggaa cgccatccct catccgcctc tgatcccatt cggggaaatc
    25081 gacgatgatc tggctgtacc tcctgccctt caccttcctc gttctccttc actcgaaccc
    25141 ggttgaagca aagatttaca gctgcgaggg cccgaacggc ggcaccatgc tgacggacca
    25201 gccgaaaggc aaacgcggct gtacggtcgt caagacgcca tcaccgtccc cgccaggcgg
    25261 gttcacgcca ccggttgaag aagcgccggc agctccgccg gacctgcagc ccaacacgtt
    25321 tctcccttct cagccggtct cgccgattca tcaacccaat ccgcgcccgc tcgatccgat
    25381 cgataagctc caaccgccaa ccggctcaca ggcgggaggg gagaaggccg caccggaagc
    25441 gcaacgctgc tcgccgcgcg tgaacccgct gaatcccttc gccggcatga attgttcacc
    25501 cacggccgac gaaacgaaaa aaccgtaggc cccttcaatt ggcagaataa cctccgccag
    25561 aacgattctc tttcacgtcc cctctcagaa gcttccccac accacgttca cgttcgtcgc
    25621 gcttctgcgt tcgagacgct gatgggtcgt ccgcaccatt gacaccgccc ccttcccgat
    25681 gtaaccattg gttacactca caaagatacc atcatgcatc gcgacatcgt ctgtctccag
    25741 attccgtcgt tcgagatcgt gctcgcgcgg actcacgagg cgtcgttgcg tcaccggccg
    25801 gtcgccctgg cgccggtcca tacgccacgc ggcctggtcc gtgaagtctc acaggaagcc
    25861 cttgacgatg gggtcctgcc gggcatgtcg gtcgaactcg cccggcaatt atgtccgggc
    25921 cttcgtgtga tcccgccgga ctcgtcgaga acgcaggcgg cgcatcgtga attgcaacaa
    25981 gcgatcatgc cctttgcgcc tgtgtgggaa tcaatccaac ccggttcgct atttctggac
    26041 ctcaccggca cacaacggct cttcggacgt cccatcgata cggccgcccg cctggaacgg
    26101 gaactgacgg tcaaatgggg atggcacagc caaatcgggg tggccatgaa taagctggtg
    26161 tcgcacctgg cggccacgac gctggagaag ccgccgcaga tgcgttcgat ctattccggc
    26221 tccgagcaag cctttctcgc gccgttgcct gccacgctgc tgccgggact caacagactg
    26281 caggcgtcgc acatccttcg ccgcttggac gatctgaatc tgagaacctt gggatccatt
    26341 gccgacgtgt cattggtcca gttggaagcg gtgctcggaa ccgcggccgt cgtgctgcat
    26401 gactgggcca tgggtattga ccactcgccc gtccggccat cggtcgaaca accgcagatc
    26461 gaacaatctc tcaccctcaa tccggacgaa atcgacgatc acctcattct gggacgactg
    26521 tcccatctgc tcgaacggat ctgcgccagg ctccggcaac aacggcgtat gtgcaggcgc
    26581 ctgtcgctgg cgctccgcca cagcgaccat gccgaacgca tggctcaaca catcttgccg
    26641 tacggcacct attgggaggc ggatcttcag cccgtgctcg cgcagctctt cgcccgatgt
    26701 ttccggcgtc gggtcaggat ccagcgaatc atcgtgcggg ccgattgtct cgaaccgctt
    26761 gccgagcaac tgcggctgtt cgacgagtcg accatagcgg tccagccggc gtcccatcga
    26821 atctcccttg tccttgatgc cattcgagcg aaattcggcg aacgggcgat cgcgtgggga
    26881 aagacgttgc catgaccacc acgcccttcg tgcacctgca cgtccattcg gattattcac
    26941 ccatgcgcgg cgtgtcgtcg ctggaaacat tgtgccgatc ggcgaagcgg caaggactcg
    27001 gcgcactggc cgtgaccgac acgaatggcc tctatggagc cattcgattt atcgaagaag
    27061 cgaggcacga aggcctacgg ccgatcctgg gcgccgaatt gaccaccgac acccaccgcg
    27121 ccctgctatg ggcaaaaact cccggcggct atgccaacct gtgccgcctg ctctccgcac
    27181 gccattgcga gccctcgttc gccttcatcg aagccgtggc gcgctatcgc gacggcttga
    27241 tcattgcgac cgacgaccag gccgctctcg aagcgtggtt ggcggatgac tcacaggacc
    27301 tgtacgtcga attgacaccg ggacccgcca tgcagaaaac cctcgagatg agccgccgga
    27361 tcggcgtccc gtccgtcgcg accaaccgcg tgtattttgc tcgagccgac gacttcgcga
    27421 ctcaccgcct gttgcgcgca attgcgctca atcagacgtt ctcccggttg ccgcaagcag
    27481 cctgctgcaa tcctgagcaa tggcttgtgc cgccggagcg gatggcggcg gcgtttcccc
    27541 atgtcccgga ggcgctggcc aatagcgtgc gcatcgccga ggcgtgttac agcgattggc
    27601 atttcggcga cacgatcttt cccgcgtttc gcgagctcac cgacaatgcc gcgttcctga
    27661 cactcaaaga cgacacctat gccggcgcgc aggaacgcta tggtctgctc tcggaagaag
    27721 tccgcgcgcg catcgagaag gaacttgccg tcatccgcgc aaaacgcttc gcgcattact
    27781 ttctgatcgt caaagaaatt ctgacgcagg agccattgac ctgcggccgc ggctcggtgg
    27841 ccgcctcgat cgtctcctat tgcctcaaga tcacccacgt ggatccgctc aaacaccact
    27901 tgttcttcga acggtttttg aatcccggac ggaaggatcc gcccgacatc gacatcgatt
    27961 ttccgtggga caaacgcgat ggcgtcctga actgggtctt ccggcaatat ggcgagcgcc
    28021 aggcggcgat ggtggcgaat cagaacagtc tggggtttcg cgcagccata cgcgaaacgg
    28081 caaaggtcta tggcatgccg gcggacgaaa tcggcaccat gtccgcgcgg gtctctcgcc
    28141 agcaagcgat actggacttt tccactccgc cgaccccgga gcaatggctc aaccggctct
    28201 cccggacctt gcggctgaag gccccgtggc ctgagattct cgaacaggcg cttcgcgcgc
    28261 agaaccattt tcgtcacctc tccatgcatt gcggaggggt ggtgatcgtg ccggatgaaa
    28321 tccgccgcta cgtgccggtc gaattcaccg ccaaggggct gccggtcatt caatgggaga
    28381 aggaccaagc agaggagtcg ggcctcgtca agatcgacct cttgggcaac cggtcgctcg
    28441 cagtcattcg tgatgcgctc gacgccattg cccgccatac cggtcgcacg atcgagtatg
    28501 cgagctggga tccgcttcac gatcatgcga cgcaggactt gatccggcgc ggtgatacga
    28561 tcggctgctt ttatatcgaa tcgcccgcga cccgccttct gctcagaaaa ttatggagcg
    28621 gcatgccgcc ggaccggctc gccgtggcgg acgtatttga atatctcgtc atggtgtcgt
    28681 cgctcgtgcg gccggcggcc atcactttcg tgcacgactt cgtccggcgt gcccatggcc
    28741 atccgtatga accgctgcat ccgctgatgg acaacatgct gcaagaaacc cacggcatca
    28801 tggtctatca ggaagacgtc acgaaggtgg ccatggcgct cgccgatttt cccctggaag
    28861 atgcggatca gctgcgcaag gtcatcagca agaaacacaa gtcgcggcag ttgcgggatt
    28921 actatcagca gttctgccgc ggcgccgcca agaacggagc ggcgcctgac accatcgata
    28981 aaatctggac gatgatcatg agcttcgcag gctacagttt ctgcaaaccc cattcggcca
    29041 gctatgccca ggtctcgttc aaatccgcct atctgcgcgc ccactatccg gcggaattca
    29101 ttgcctccgt catcagcaac cagggcggat tttattcggc cttcgcgtac gtctccgaag
    29161 cgcgccgcat gggactcgcc atcctgccgc ccgacgtcaa cgccagtgac tgggcctatc
    29221 gcggcgaagg cgatcgtttg cggatcggct tgatgcaggt caagacgctt cagaaagagc
    29281 tcggcgaacg tatcgtggag aaccggacag aagccgggcc ctatcgctcg ttcgaagatt
    29341 tccgtcggcg cgtcaacctt cccccgacgc aggcgcgcgc gctgatccga gccggctgct
    29401 gcgactccct cgccggcgaa ttgacgagac ccgccttgct ctggcggctg tatgcggagg
    29461 agcaacgcca cgccgggccg ctcccgatcc cggaggacta tacggacacc cataagctga
    29521 cccatgagat cgagtcgctc ggctttttga cgaaccgcca tccgttgacc ctctatcgcc
    29581 attcaatcga gcggctgcgg cccgtgccgg cggcgcacat gcatcgccat gtcgggcagc
    29641 ggattacgat ggtgggctgg ctggtcacgg aaaaaccggt ggagacgcgc cgcggacagg
    29701 cgatggaatt tgtgacgctc gaagatacca ccgccctcta tgacgccacg ctgttcccga
    29761 acgtctatcg ccgctgccat caattcctgg cacccaatcg ggcctatctc gtgcgagggc
    29821 tggtggaagc cgtcttcggc gtggtgacat tgaccgtcac cgagttgcag ccgctcgacg
    29881 ccgaaccggt acgcgagcct gcatggaacg gcgctcggga cgcatggtat ggtgaatcat
    29941 gcgacgatcg ctccgatcag ccgcctcctt tctcccttcc aaactgaccc tcccgacgct
    30001 gcggaacgcc gccgcctcct gcacgggctg cgacctctat aaaaccgcca cgcagacggt
    30061 cttcggggaa gggcctgctc gcgcgacact gctgctcatc ggtgaacagc cgggcgatca
    30121 ggaagatgtg gccggccacc cctttgtcgg acctgccggg agagtgctgg acaaggcgct
    30181 ggaggaagcc ggcatcgccc gcagcgacgt ctatgtcacc aacgccgtca aacatttcaa
    30241 atgggagccg caaggcacac gccgcaagca caagaaaccc tccgccgccg aagtcgcggc
    30301 ctgccggccc tggctggaag cagaaatcca ggtgctggag ccacgcgcca tcctctgcct
    30361 gggcgtcaca gccgcccagt cggtcttcgg caaaagcgtg cgcgtgaatg aactccgcgg
    30421 acgccccctg accgcgccgt tgggaaagac cgtgttcgtc acaacccatc catccgccat
    30481 tctccgcggt ccggatccag cgcagcgaga ggaggactac cgccatttcg ttggagactt
    30541 acggctcatc gcggaatacc tccagcaacg gcgctgacga caggttgcac gagcctacgt
    30601 atcccgctcg ccaccgctga gcaacctgac gcgcatgcat cgctcgattg actctccttc
    30661 tgccccttca tataatgccg aaccaatcgt ttaagctgat ctgacaagca cttatggctg
    30721 tcttctcatc cgggttattc tcccgtcgca cccaagatct tcgagccggt ctccggcacg
    30781 cctttgccct gcgttcagac caaccggcct tcaccaccga ggatctcgcg ctgctcgaac
    30841 ggattgccga cgcgatcgtg acgcgccgta tggcggcacc ggccacgctt tttctcgagt
    30901 cgatgggccc catgaatttt ctcggcagcc aggcgctgca cttcctggct cctctcgtcg
    30961 aatgtgtctt cagcagcagc gaactcgcgc agatcgcccg cctcctggag cgacgggatt
    31021 ctctacaacg cctcaccgcg ctcatcgaag ccaaggccgc agcgtccaaa ggagcgccgg
    31081 ctcgatgacg gcagcgcatc agtctcccgc gacatcgaat cccgcgcagc cgctgaacgt
    31141 catcgtggcg accgactgcg gcagcaccac caccaaagcc atcctgatcg agaaggtcgg
    31201 caacgaatat cgtcagacct accgtggtga agctcccacg accgtggaag cgcccttcga
    31261 ggacgtgaca cgcggcgtgc tcaacgccat tgcagaaatc gaagaacttt ccggccgcac
    31321 gatcctggac ggcgaccgca tcatcacgcc caaccaggcg gcccaaggcg acccgcagcg
    31381 aggcgtggat atttatgtgt ccaccagcag cgccggcggc ggcttgcaga tgatggtcac
    31441 gggcgtggtg cagaacatga cgggggaaag cgcccaacgc gccgcgctcg gcgccggtgc
    31501 catcgtcatc gacgtgctcg cgtccaacga cgggcggttg ccctacgaaa agatcgaacg
    31561 gattcgcacc atgcggcccg acatgattct catgtccggc ggcacggacg gcggcgccgt
    31621 gacccacgtc gtggaaatgg ccgaatacat cgccgcagcc gaaccccggc cgcgcttcgg
    31681 gagcacctat cggctgccgc ttgtctacgc aggcaacaag gatgcgcagc cgcaggtcaa
    31741 aacgattctc ggcgagaaga cggccctgga atttgccgac aacatccggc ccgtcctgga
    31801 gcgggaaaat ctcgcgccgg cgcgcaataa gatccatgac ctgtttctcg aacacgtcat
    31861 gcagcaggcg ccgggctaca agaaactcat cgagatggcc ggcgcgccca tcatgccgac
    31921 gcccgccgcg gtcggcgtca tcatggagac gatcgccaag cgcgagggga tcaatctgat
    31981 cggcgttgat atcggcggcg cgacgaccga cgtgttctcg gtattcggcg gcctcttcaa
    32041 tcgcaccgtc agtgccaacc tcggcatgtc ctacagcatc tcgaacgtgc tggcggaagc
    32101 gggcctcgaa aacatcatgc gctgggtgcc gttcaccatc gacgaacaga ccctcaggaa
    32161 tcgcattaag aataaaatgg tgcggcctac gaccattccg caatcgctcg atgagctaca
    32221 gatcgaacat gccatcgcgc gggaggccct acggctggca ttgatccacc ataagtcgct
    32281 ggcgaccggg ttgaagggtg tccaacagga acgcaccatc tccgatgtct tcgagcaacg
    32341 cacctccggc aagacgctca tcgatttgtt gaagttggat ttgatcgtcg gcagcggcgg
    32401 catcctgtca cacgcgccgc gccgcatcca gtccatgttg atgatggtcg atgcctacga
    32461 accgctcggc gtcacgacgc tctcggtgga cagcattttc atgatgccgc atctcggcgt
    32521 cctctccacg gtcaacgaac aggcggccac cgatgtcttc gtccgcgact gcatggtgta
    32581 cctgggaacc tgcatcgcgc cgatcgggca gggcaaggac ggcgagcgat gcgccgacta
    32641 cgaggtcgcg ctgccggatg gacgcatcga aaagggcacg ctgacgttcg gcgatctcaa
    32701 gttattcagt ctgacccgcg atcagcaggc aaccgtcacg ctgcagccgt cgaaacaagt
    32761 ggatctcggc gaagggccgg gacaatcgtt cacgcgaacg gtgaagggcg gggtcgtcgg
    32821 tctcatgctg gacgggcgtg gccgtccctt gcagttgccc acggagcagg ccgcgcgagt
    32881 cgccctgctg acacgctggt atcaggccgt cgggttgtac ccgacgggga gctcatagtg
    32941 agcgcgtatg gctcatgacg agagacaaca tgcggccaag cccgcaatgg acatcattgg
    33001 ctttaagagg ttgctcaaca cgtcttgcag caaggccgca aggcgccaga gccccgagtc
    33061 gtactcttga tgtacggcga ggggaatgag caactgagaa cgacactgca aggcgtgttc
    33121 agcaaccgac catatggcac atagttacac accaggcctg accgtcactg aacggacgac
    33181 cgtccgccgc cggcgtattc tccctttgcc gggtgccgtc ttggtccaag tcggcgatgc
    33241 tgtgcgagcc gacaccgcgg tggcccgcgc tgagttaccg ggaaaggtgg tgccgctcaa
    33301 ccttgcgaat cagctcggca tcgcaccgga tgagatcaac gactatctgg tcaaaaagga
    33361 aaaggactcc gtccagaaag acgacgtcct cgccgagaac aaacccttca tcaaatggtt
    33421 taagacggaa attcgctcgc ccatcacggg caaggtggaa tccgtctcca cgatcacggg
    33481 acaaatcctg ctccgcgaac caccgcgagt gctcgagttg cgcgggtaca tcgacggcgt
    33541 cgtggctgag gtccatcccg aacagggtgt gacggtggaa agcacctgca gcctcgtgca
    33601 aggcatcttc ggcatcgggg gagaaaccag tggcgaactc gtcatgggtg tgaaggcgcc
    33661 ggatcagccg ctcctgccgg aacagctcaa cccatccatg aagggcaagg tcgtcgtcgg
    33721 aggcgcgttt ctctcggcgg ccacgatgac gcgcgcgaaa gaactcggga tcgtcggcct
    33781 ggtcgtcggc ggcatccatg acaaggattt acgcgcgttg ctcggatatg atctgggcgt
    33841 cgcgatcacc ggcacggagc aggtcggctt cacgctgatc ctgacggaag ggttcggcac
    33901 gattcccatg gcaccgaaga cgtttgcgct gctctccgcc catgcgggac aaaaggcgtc
    33961 gatctcggga gctacgcaga tccgcgccgg cgtgatccgc ccggaaatca tcattccaca
    34021 cagcggcccg gctgcggtca cgaagctgcc caaggccgaa cgggaaggca ttcaactcgg
    34081 cgatccggtc cggattatcc gcgatccgct gttcggcaag atcggcgcgg tctccgggct
    34141 gccgtctgaa ttgcgcgcca ttccgaccga aagcgaagtc cgcgtgctcg aagtccgatt
    34201 tccggacggc acgacaaccg tggtcccgcg cgccaacatc gaagtgattg aaggagcctg
    34261 accctcttgc tgcgccgcat cctcatcctg gtctttgtta tcagcttgcc ggcaatcatg
    34321 ccgacgctcg ccccggcgca ggccatgccg cagcaacttc gcgtgatcga tacccccagc
    34381 gacggaggcg gaagcctcac ggtgctctgg gctccctcgg caaccgacag tgccaccacg
    34441 aaatatcaaa tcctcgtcca tgagggcggc cttccccagg acccaacggc gtggaaggtc
    34501 gtggcagaat ttcccgccaa tacgcgctac gtccgcgagg gcaagtcggc ctggtggacg
    34561 agatcaggtg atccgaacga tcacctcttt ctaatcaaga atggcaaggg tctcgaggtg
    34621 aaatccggcc agccctatgc cgtcaccgtc gcgtcggtca acgggaagga acgattgatt
    34681 gcgccgcccg taatcgcctc tgcagaaccc aactggatga actggaacca ggtcaacaat
    34741 cttgtgttgg cgttgatgtt cggcgcgatc gtgttttacg ccatcaatct ggccaagcgg
    34801 aaggacatct ttctccgccg cattcccggt ctggatgccg tcgatgaagc gatcgggcgg
    34861 gccacggaat tgggcaagcc ggtgctgtac atgaccggcg cgcatgacat gaacgatccc
    34921 tccacgattg ccgccgccgt gatcctggga cgtgtcgcca agaaagccgc ctcctacgaa
    34981 acggaattat tggtgccgca tcgagagccg atcacgatgg cggtctgcca ggaaattacc
    35041 aagcaggcat acatggaagc ggggaaaccg gatctgttca aggacgacgc gaactttttc
    35101 atcactagcg accagttcag ctacaccgcg gcggtcgacg gcatcatgct ccggcggaaa
    35161 ccggcggcaa actttttcat gggctcgtac tttgccgaat cgctgctgct gacggaaacc
    35221 ggcgccagca caggagccat tcaaatcgcc ggcaccgatt cggaccacca gttgccgttc
    35281 ttcgtgacga cctgcgacta caccctgatc ggcgaagaac tctacgccgc cagcgcgtac
    35341 ctctcgaagg agccgatcca gatcggcacg ctgcgcggac aggatctcgg caaggccttc
    35401 attctgagcg tcatcgggat cggaacgctg ctggccaccg tcgccgtact caccgggaac
    35461 gggtgggcgc agccgctgct ggacctgttt aaggatctca aatgattttc cttcgccgac
    35521 aactgccgct gcttatcacc atgatcacgg gactggtcat ggcggcccaa tactacgtgc
    35581 cacatccggc ctccgaacaa ctgcttacca ccgtgacgaa gtggctccag atcatcggcg
    35641 gcttcgcgct cgtcctcggc atcaccagcc tgtttcatgt tcatgcggcc aagatccggc
    35701 ggaaggaagc cgggtgggcc tatagcctgg tgctttatgt cggcatgctc ggcacgatca
    35761 tcgtgggact ctggaataac ggcaaggaaa gtatcgacgg cgcgttaacg tcgttcggct
    35821 gggtctactc ctatacgctc gtgccgctgc agggcaccat gttcgccatc ctggccttct
    35881 tcatcgcctc tgccgcgtac cgctcgttcc gcgcgcggag ccgtgaagcc gccgtgctgc
    35941 tggtggccgc cgtcatcgtg atgatggggc gggtgccgct cggcgaatac atcctgccgc
    36001 tcagcggcga cctgtcgagc tggattctga atgtcttgaa cgcctccgtg cggcgggcga
    36061 ttctgatcgg cgtcagcctt ggcgcagtcg ccctttcgtt caagatcatc ttcggcgtcg
    36121 aacgttcgta cctcggcggg ggcaaggaat gacgttcgcc gagcgtatgt tgcgcatcga
    36181 ccggcggatc atctttctgg tgatcggcct ctgcacgctc gtgccgctgc tgttcccggt
    36241 cggattaccg atcaagattt cgtccgaagt gcgcggcgtc tacgactata ttgaagggct
    36301 gcccgaagga tcggtctttc tcttgtcgtt ggacttcgac cccgcatcca aaccggaact
    36361 ctacccgcaa gccatcgcga ttctgcggca cgccttcaag aaaaatctcc gcgtcgtagc
    36421 gatgacactc tgggtctcag gcaccggact cgccgaccag atcctgacgc aaacagccaa
    36481 ggaggcgggc aaggagaacg ggaaggacta cgtcttcctc ggctggagtc ccggcggcag
    36541 cgccgtcatt ctcaacatgg gacaggatct ctacaacgcc tttccagcgg actacggcgg
    36601 caaggcgacc aaaggcctgc cggtgttggc tggcgtgcag aacctgcgcg acgtcaccta
    36661 tgccgtgagt ctcggtgccg ggaatcccgg cgtcgaagcc tggtacgtct tcggcaaaga
    36721 caaatataag ttcgaactcg gtggcggctg caccggcgtc atcgcgccgg gcctctatcc
    36781 gctgttacgc agcgggcaga tcaacgggtt gatcggcggt ctgcgcggcg cggcggaata
    36841 tgagaccttg atcgggcaga aggggaaagc ggtcgccggc atggacgcgc aatcggcgac
    36901 gcatttggcc atcatcgtgc tcgtcgccat ctgcaacatc ttttatttct ccttgcggcg
    36961 gcaacagcgc cggcaggacg ggatgggatc ctaggcacta tggaactgtc gttcggtgtc
    37021 atactcggtg cctggatcgc cacaggcttg accctcttca tcctctcgtt tctctatgag
    37081 gacaatccgc tgttcaagct ggccgaacat ctctatgtcg gggtctcgct cggctatacg
    37141 atcgtgaaga cctatgacac ggtgatcatg accttgatcg tgcgccccat tctcgacaag
    37201 ggggaatggt cgctgctgat tccggtcggc atcggcatgc tcatgctcac gcgttatgtc
    37261 ccgaaggcag cctggctgtc gcgctatgcc ttcgcgttca tcgtcggcgt cggggcggga
    37321 ttggcgattc cgcgaacgat ctcgtcgttc attttgaaac agatcgagga taccgtacgg
    37381 ccgctcctgg gcatcgcccc cggcggaggc gtgaccttcg actattcgct gctcaatccg
    37441 gcgagccatc tgaacggcat catcatcctg atcggcgtcg tgtccgtgct gttttacttc
    37501 ttcttctcgg tggaacattc cggtccaggc aaggccgtcg cacgcgcagg gattctcttt
    37561 ctgatgattt ccttcggcgc cgccttcggc tacaccgtga tggcccgcat gtcgctgttg
    37621 attggtcgcc tgaccgatct gattgaattc tcagacgcct cctacggacg ccccacgctc
    37681 tggctggtgc tcctgaccgt cgccactctg attgtcctca gccgccgcgg cagcacccat
    37741 ccgccgaatc agtaggcgcc cggtgccgtt gttcgtccct cgtctctcgt cgttcgcaag
    37801 agcagctcgg cgacgaccct gcgcgtcatt tcgtccccac gagacgcttc acgagatacg
    37861 aaatacggtc tcctggagat tggcttcacg cttcacgaac gacgttcctc aaggctgttg
    37921 cgcctgagaa gtttgctccg ttacaatgcc gccactatgg aatccacac
//
LOCUS       DAOQDS010000041        36033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_404_length_36033_cov_18.604758, whole genome shotgun sequence.
ACCESSION   DAOQDS010000041 DAOQDS010000000
VERSION     DAOQDS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 36033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36033
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_404_length_36033_cov_18.604758"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..1005
                     /locus_tag="PK589_11470"
     CDS             40..1005
                     /locus_tag="PK589_11470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNJ20267.1"
                     /translation="MAAPTLYISRLLPDPVMTAARRQFQLLNDPRDASPSWDNVKTGL
                     READAAICTLTDRVDASMLAEATRLKILANYAVGYNNIDLAAAKARGIVVTNTPDVLT
                     DSTADLTWALLLAVTRRVAEGDAYVRSGDWSGWAPTQMLGTDVSGKTLGIVGMGRIGQ
                     AVAQRAAGFNMRILYTSRTPTALERVSSQWESRTLPDLLKEADFVSVHVPLTAATHHL
                     IGARQLALMKPTAFLINTSRGPVVEEAALVDALLQRRLAGAGLDVFEHEPAFHPSLRE
                     LRQVVLLPHLGSATLATRVRMGMICLENIAAVVAGKPAPNQVHAA"
     gene            complement(1061..>1584)
                     /locus_tag="PK589_11475"
     CDS             complement(1061..>1584)
                     /locus_tag="PK589_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ20268.1"
                     /translation="PAPVVEVEPVIAAAPPPQAPPPVAPPAPVVEPIDAETRYALGMA
                     YKDMGLCEEAKEEFILSMKDSGFFVDSCLMMALCCKEQSRPDQAIQLLEKLISDSRCR
                     GGNAQLVRYELALLYEMTGARDRALTMYQSIPSFHDVPRRVEALRMQSTNGAAHPELP
                     ASTPTSPLPIAGH"
     assembly_gap    1585..1684
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1685..3177)
                     /locus_tag="PK589_11480"
     CDS             complement(<1685..3177)
                     /locus_tag="PK589_11480"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ20269.1"
                     /translation="MAVDRRTVLQNAQLFASKGQYDAAIAEWRKLIADTPNDGTVFNS
                     IGDLQLKRNATAEAVTAFLQAASAFRSEGSVLKAIAAYKKILKVDPARIEIYRHLGDL
                     NAERGLLSSAVQDYLTLGKHYFKDGRNKEALEIYRKIVSQDPSNLDAQLRVAELCVQE
                     GLVDEAVRMYLQLGREQSAAQQYEQAKAMYTAVLRLDPENAEAKEITSMIETGGSVTP
                     SAARPAKPATTAAKPGDASDLMAEATRRIEEGQYAGAEAMLTQLLSREPGNPEICQLL
                     ARMHLLQGNLVVALGEYRFLAGAALRAQDYGVAESLIADYLKVEPASVPMLELLGELY
                     QEKGDAATAAQHFGRAVEILLEHPEPGMPTLPAELFEKVQALAPGSPIARKLAPAFDP
                     NAGSMPEPEPIAPVMEVVLTPAPEPVVEPAPELEPTPVIMAAAPPAEVPFRFVGDPAP
                     PLEAAVVKPPNVEPVVEPVVVQAAPPPEVPPAPVVEVEPVIAAAPPP"
     gene            complement(3357..3575)
                     /locus_tag="PK589_11485"
     CDS             complement(3357..3575)
                     /locus_tag="PK589_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNJ20270.1"
                     /translation="MKTKGTVKWFNDRKGFGFIRLDGGDDVFVHYSALQGDGFKTLKE
                     GENVEFDIVQGAKGPQAANVLKDLAPAN"
     gene            3888..4973
                     /locus_tag="PK589_11490"
     CDS             3888..4973
                     /locus_tag="PK589_11490"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNJ20271.1"
                     /translation="MLHTILDRYIFRELLSPFCISLGALCFVMLTKELLRLVELLVTK
                     GIGFLSVLKVFAHLLPSFLVLTLPIAGIIASITAFGRLSLDKELVAMRAAGFSLLRLS
                     QPVFLFAALVCGLTLMMAQYGQPWSNVNLKKVALNLLRDELVLELDRGVFNEAIPKMV
                     IYVPDAQEGQDNRGIFVADERNPADPRIIVAQQYQVMTDSASSQVALRLMNGVIHSRP
                     QNPEEYQKISFTAYDLKLSINASLYGAEERTPMDVIRAKLESSNWTDTNALRRLMEYY
                     KDLAFPAASLVFCILGVPVGIVSKRSGSIGGFAVGVLVVIAYYVINVACEFLVTTLWI
                     SPFAGAWLPNVIFTIVTILWFYRVSRQ"
     gene            4978..6069
                     /gene="lptG"
                     /locus_tag="PK589_11495"
     CDS             4978..6069
                     /gene="lptG"
                     /locus_tag="PK589_11495"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNJ20272.1"
                     /translation="MNILFRYMLREYVKIFGMCFSGLMTIYLVIDFFEKVRRFLRYDA
                     HALDVLAYFVLKMPAISYQIAPLAVLMATLLTIGLLSRSHEITAMRSCGISLYWITSP
                     FIFLGTVLAMVLFLFSSTVIPLALAKADQIKTTQIEKRVTPVSLKAAQPWVSLGGQTL
                     LNIDTIDPGGAVLRNIRLYRLSPTFQLEEIAEAKSAVYSSQGWALHDGMTRSFRQDGT
                     VLVSSFQTQPLALPHIPDDFTTWAELDSETMTLREIRAYVDRLHLGGTMLPRLLTDYY
                     GRIAFPCVTLIMVIVGIALSLRRTGVRGSGMAMGIGQAMAVGFFYWSTHSVAIALGRG
                     GALSPMLAGWMANMVFLTFGLYLLMKVRY"
     gene            6322..7578
                     /gene="fabF"
                     /locus_tag="PK589_11500"
     CDS             6322..7578
                     /gene="fabF"
                     /locus_tag="PK589_11500"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNJ20273.1"
                     /translation="MSTRVVITGLGVVSPIGIGVPQFWKAALEGRSGISAIPSFDPFP
                     LEGYRSRIAGRIVDFSPDQYLPAGQGDRVDRYAQFALVAAKEALADADFKMERENPHR
                     VGVIVGAGMGGMVMGEREITQLYEQKRPHRVHPNFIPVITLNSASGIVAMAYGAKGPN
                     LTISTACSSSAHALGQAMHAIRAGTADAVIVVGADASITPLVFAGFCSLRALSTKYND
                     APEKASRPFDRGRDGFVMGEGAGALILESLAHAKKRKARMYAEVAGYAATSEAHHMVI
                     PREDGEEVATTMRLALKDAGVTPAQVDYINAHATSTTVGDAVEVKAIRLVFKSRADKL
                     AVNATKSLVGHTLGAAGSLAGVVSALTLTSGQIHPTLNLDDPDPACALAGLSKDQQTR
                     KAKVALINAFGFGSNNAAVVLKAVSS"
     gene            7641..8000
                     /locus_tag="PK589_11505"
     CDS             7641..8000
                     /locus_tag="PK589_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNJ20274.1"
                     /translation="MATTTDVSSKIIQALAEYLKRDASSITPTHHLREDLGLDSMAVI
                     EMLYRIEEVFNLQIPDQDLVGLTTVGHVIAYVEGRVAPASTKTAAKGAAKPSAKTPAK
                     PAAKKPAAKPSPESKKA"
     gene            8005..9087
                     /gene="lpxD"
                     /locus_tag="PK589_11510"
     CDS             8005..9087
                     /gene="lpxD"
                     /locus_tag="PK589_11510"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNJ20275.1"
                     /translation="MSTAPRRTPLTLRELHDYVGGELIGSPDATVTAVASLEQAGPGD
                     LAFVTSERHLKAPQTATFGALLVGRRIADCPSPQIVVENPAYAFARAAQQFFARPARV
                     RGIAECLTRGDGVTIGTDVSIWPGVTLGDRVTIGARVTLYPGVFIGDDTSIGDDSLLY
                     PNVVVREGCRIGARVIIHSGTVIGSDGFGYVQYQGRHQKIPQLGGVLIEDDVELGANV
                     SVDRATFGDTVIKRGTKIDNLVQIAHNVVVGEHNILVAQVGIAGSTTLGRYVMVGGQA
                     GLADHLQIGDQVMIAAKSGVTRSLEPNQIVSGAPVMAHATFLKAQAVIPQLPELRNRV
                     RELEERLATLEQAAKPAAKTRKPRRK"
     gene            complement(9105..9629)
                     /locus_tag="PK589_11515"
     CDS             complement(9105..9629)
                     /locus_tag="PK589_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20276.1"
                     /translation="MPKLVSVKQQEGHDARATAYIKAYMLFPAGILGLISMLGGVGGL
                     AYQLIATDTYSWTTFVESSGLLLLGGLLGWVQTTYHRWILANRPEVFAERMRQPALTK
                     SGKPKRESGKNQESSSGSPWAPGAYMVGLAVLLAGSMLSMLYGAVHPIPACFLPWAGF
                     FWAKLFFWKTLLKP"
     gene            complement(9882..11969)
                     /locus_tag="PK589_11520"
     CDS             complement(9882..11969)
                     /locus_tag="PK589_11520"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNJ20277.1"
                     /translation="MEQNHYRHAEEILAKVAASVQRQEDVDLTELTHLADSIVESLQG
                     SDQLVVEALSSPSGPPLVTNLINVGILATKVGIGLGYYGVELHRLAVAGLLHDIGIFA
                     VPQQLLTKAGRLTAEERTLVEQHPRLGSEVIRRVGTEYGWLAEVVLQAHERGKGQGYP
                     HRLKGREINELAQIIGLVDIFDALVSPRPYRRRLLPHEAVRELLTTERTAFPREIMKA
                     LVEQLSVYPLGTRVRLSSGQEGVVVRITARYPSRPVLRITSTETAAPSEPPHLLDLSL
                     LPHVSVVDTVEPPALDRVTFEAAGAAPAASAAPTNVSDQFAALLESLDAIAGVIQAAV
                     DQPAVARGPAQVPAEAETAEPLIPVRREILGLFVLEAREWLNQIQTALERLDATSEQA
                     RRAQLASILWQSVNNLARSAATVGLTAVETMATRLFPLLQAAARHERAVAAHHVASLR
                     EGLLEISKTVQELRPIEEHPAPAEETVEREQPILEQPARTELPESQASSEPASEIATP
                     RYAPPRPMPASSILDALRQLHVARGRSLEPVRDVLETVIQRAERELAQGAPVVDARAI
                     GRLLQELDELDERFLNHMQARVPSVVSTLGRIARASEERVLPPQALEPIFEEIDVLFE
                     ASEKVSAENISVFLQGLRTFLRVTAQHKPAVIRERLAAVEERLATLIPLAQQWVDVGR
                     VERAAIFDILPMA"
     gene            complement(12152..13099)
                     /locus_tag="PK589_11525"
     CDS             complement(12152..13099)
                     /locus_tag="PK589_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20278.1"
                     /translation="MSTEPEQTQDDFQKELIELFGQEAQEWLVQIHAALVELESLPDS
                     DRHSQLIDAVVRGITSLGGSAATVSLPDVERATFALLPFIETIKDRTTATKQDFSTVR
                     EHFRTVIASVKVATGITLDIDPLPEAAPAPEPVLEFLPLLNALHQLQDEQAAQGSMSR
                     SLIPQVLQRLEHEARIGAGQITAAQFQELLLTIHNTDAQYLGSLRQDLPGIALHLSRL
                     RAGGVAVLTSDAGFDAPIQMIASLHEIAKQTNATPLVTFLTGLQNFLTLVVQRRIDIA
                     AHRLQSVETRILAVASMVEEWIADGQKEFDMMSRLAPST"
     gene            complement(13156..14631)
                     /locus_tag="PK589_11530"
     CDS             complement(13156..14631)
                     /locus_tag="PK589_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ20279.1"
                     /translation="MPKILIADDSIAVRKVAERLLTEAGMGVTLAANGSEALAMLAKD
                     RPDLIVSDVIMPDKSGYEVCAFIRGQANLADLPVLLISGIVNDEVSKQAESCKADGVL
                     KKPFQGSSLKDRVLDLLTKRPHKPAPVAEPIRIPIAEPPVMAEVAPAPVAPAQEELPS
                     IPLVQREWQQPAEPVAIVLDQTPAGPVGIEESRAGSISIHQAAEPSPVMQADDRHQAL
                     LADRDAKIAELEAQLGAERRHSQEEREQHQLTVSEQREQMADVSSRMQALEQDLAKER
                     AQRESMTQQLEDLSRQAHRLGEVEAALLEERHRADQLAQQAAESARLGARIVELEAHL
                     EAEREAANQLVNQITSLEHVEDRMRELERVLASEREEAMALRNERAHLEKSAAMVPVL
                     EEAGEHARAQVAELERTLTAEREAAAALLAQYKQMEEKAQLVPDLEQELSAERDHSMQ
                     LTKQVADAESMAEQSTRRFEDLARKLGEIAGLASQLGNAKR"
     gene            complement(14660..15136)
                     /locus_tag="PK589_11535"
     CDS             complement(14660..15136)
                     /locus_tag="PK589_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNJ20280.1"
                     /translation="MLRSQLRRHPHTTASGAAHARVQNMVVFSVAGKRLAARTEEIGG
                     VLPWPGTTAVPSDTPLVTSLVRHEESCLPVFDLAAKFNRTVREEESLCLIVKHVDGPL
                     AICIDSQVPSLHVVPRSGVQYQSGLDPDIAGSCVAGDEQLPLINLATLGVASQRPA"
     gene            complement(15152..15724)
                     /locus_tag="PK589_11540"
     CDS             complement(15152..15724)
                     /locus_tag="PK589_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20281.1"
                     /translation="MGLRGQRAVTISSGQAVRFLVTLIGGSSVAFPAQWVRGIVMPAS
                     AGPNGLVTWAGVEYERTDLSARLKVVSKGVSSDTRLILYGNDERSRSFAVDKVVGLLD
                     VERALIHPLPPQFRGKERERLLGFFVGASFVALIANPLWVLELPLRAQTLEMFAIRFA
                     ESKAGESDSRLHVPMVGVDEAMSMSTSPVS"
     gene            complement(15772..19152)
                     /locus_tag="PK589_11545"
     CDS             complement(15772..19152)
                     /locus_tag="PK589_11545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNJ20282.1"
                     /translation="MSADFDRDQLLDIFVAEAGDDMERFWKALHPEGKGRPEPAEVAE
                     FHAVGHKLKGAALLYGFPGLGQLGALLEETLEHVQEMSPSQWPAAFEVIRDIVASFRH
                     QVTRIGRGEGEDASLVEGFVQRCAQFLPAASADDSSQCLPIATEPADDYLLPVMDEEV
                     LSYFAPEAEEYLNTIQTLLQRLELDLSDAETIHQLYRVAHTLKGSAYTVGFQVVGDVA
                     FPIETCMIAVREGAAVIGPQWIATIRHAVDVMRSLMARDAQQLPRLRQDVPCMTTMLR
                     ELEQGMTQAPQVDAPAASSSSVAGEEALPPAAKPLNPAAQSSEPNHPAELSEAYLIPM
                     LDAEVISYFAPEAQEYLESLETDLLRVDKDAANPDIIHQLFRTAHTLKGSAYTVGFQS
                     IGDLTHHIEDFMGAVREGRLKFLPGHTDVLLRAIDVIRALMRRDPSLVTRTRQRFAAS
                     LQELKGLGEAAPAGVHEPVMSSQTEAVIALDVQEAEQAEPVNAAEGKAGEEREVIRVS
                     RDRLERLLNLVGELVIDRGRLEQRLRTLDQLATQVLANKNRLTDAVRTFEDKHTFSFQ
                     PSPTTQGEAMPQGFPGVSDFGSLEFDKYDDFNILARRISEVTADISESMSQLSGSIHR
                     AQDDMGSLQQLTLGMRDEIARARMVPIGTPFTRFRRAAREMARATGKDVNLVTSGEHT
                     EIDTGVVERLVDPLVHLVRNAVYHGIEPAAVRRVQGKPETGTIYLHAAHRGNSVLIEV
                     EDDGAGLDIAKIKAKAVKLGLVRQDVAETLPESEVIKFIFLPGFSTADAVGDQAGRGV
                     GMDVVKRVIETMNGHIEVESVRGQGTKFTMHLPLTLLIATALLVRVGKDRYAIPLPSV
                     REVTMSTASTLQHMGDRQVVQIGDEAIEVYPLGSLIRREAGITDGVSPVVIVRTSTGA
                     LGCAVDELLGRQEIVIKSLGGLKPYERSVFGGATIDPEGRVVLVLDVSRLTTREYQET
                     MSIAQDSSVAAIEDATGHSEPKASAPSALPLLLIDDSLSIRKFVGRMLEAAGYTVETA
                     TDGEEGCRKAMVQAYQLIITDLEMPKLNGYEVIQALRARPQTHATPILVMTTRAGEKH
                     RQMAVSVGASGYIAKPVEERALIQEVRKWTGVEARAEK"
     gene            complement(19205..>20511)
                     /locus_tag="PK589_11550"
     CDS             complement(19205..>20511)
                     /locus_tag="PK589_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNJ20283.1"
                     /translation="QVSSIESVAKDLNDDAQSLASNGTFILVAGGLVAVALGLFVGYL
                     LATFLSRNISHIANVATQAAGGNLQARAKIESHDELGQMATAFNSMLDRITALVSTEE
                     ERDLMQKRLMQFLVLVSEVGKGDLTRRGEVTADMFGNLADGFNLMIARFGQLLKQVRE
                     AADRVNKSAGTLRDSAGQMSGTARAQAEESVRTLGAVEQLAAGMRQVASTAGASSDSA
                     KQVLSATERGNVAVQETVRDMQSIRSAVQRMSKQVKGLGDRSLEISQIVSTIRDIANQ
                     TNLLALNAAIEAAGAGEAGARFAVVADQVRKLAESSTQATREIADLVKVIQTETQDAV
                     VAMEHETQAVEAGSASALRTGDVFAEISDIAKRSSELAQNIASAASEQTTSTEKVGRA
                     IKEFTGGAVATQKQTDSTRLTIEDMAKLAEGLNSSVAQFKLA"
     assembly_gap    20512..20611
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20612..21198)
                     /locus_tag="PK589_11555"
     CDS             complement(<20612..21198)
                     /locus_tag="PK589_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HNJ20284.1"
                     /translation="MKTRSKLLLSFGLVSFIIMIMASVGVFTLRQLSAQSQTVYVDYT
                     VPLAEFAQMGTALTKHHQILLDVASATKQSDFAQEITKLPPLKAEIEKAINNYKSTNL
                     RVSRSGRDEMKDLTLFEPALKKYFQEADGALSAMADSFDRNTLSPAQAEQMRALGILA
                     LTVNLTPSFENAVRRHNEQVSSIESVAKDLNDDAQS"
     gene            complement(21330..21875)
                     /locus_tag="PK589_11560"
     CDS             complement(21330..21875)
                     /locus_tag="PK589_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNJ20285.1"
                     /translation="MSDVPIIQPQASATSPAQGSSGSSRTSAASTEGALRMCVLTLGG
                     ELFAIDLRHVSEVFEVEAVTAVPSMPSYLTGVTNLRGTVITLVDLRGSLGLSVNNTAL
                     PFAVVIRHGARQVGVLVDHVPEIHTVPREHLLPAMQAGPAGARPCVSAVLRVDHRLGG
                     VLEVPQVFAQVDGGSAALSVV"
     gene            complement(21879..22241)
                     /locus_tag="PK589_11565"
     CDS             complement(21879..22241)
                     /locus_tag="PK589_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ20286.1"
                     /translation="MSKIVVVDDSYAELQLIEGYLKSANHTVVSYPNTDKLEDKLALD
                     KPDLIVLDVVMPGRNGFQACRDLKSDDRFKGIPIVLCTSKGQESDKFWGQQQGANGYV
                     VKPFKAEDLVAAVKRALN"
     gene            complement(22307..23041)
                     /locus_tag="PK589_11570"
     CDS             complement(22307..23041)
                     /locus_tag="PK589_11570"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNJ20287.1"
                     /translation="MANHFFHRIAMAVLAAACVGGPVAASANTEVETAELLIKLLQVG
                     RGVLSENQATINDASKGDKGFTGDYVANQVIDRFRKATKIDLSRPATVPQAPLFLAMV
                     ESEKEVIDEAQPVINKQGVGFKGIIPAVFARKSGERFYRKTGIRVKLTSIDYRFPGNR
                     PDDFESEVLRIFSDSRHPKGQPYSKATMLDGRPVLRMMDPEYAATTCLSCHGNPKGER
                     DVTGAKKEGWHEGDLAGAISIVIPMK"
     gene            complement(23451..25004)
                     /locus_tag="PK589_11575"
     CDS             complement(23451..25004)
                     /locus_tag="PK589_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HNJ20288.1"
                     /translation="MPHPSRAKAIAILGTGSDVGKSMVAAGLCRLLHRAGIRVAPFKA
                     QNMSLNSFATPEGGEIGRAQALQAEACGLAPHVDMNPILLKPESNARSQVVVQGKVFA
                     TLDARDYFTRGQDSRLLRAVRESYERLAAQYEVIVIEGAGSAAEVNLRDRDLVNWTTV
                     ELADARVVLVGDIDRGGVFAQIVGTLDLIAAEERARVCGIVINKFRGDRTLFDEGVRF
                     LTARTGIPILGVLPYLRDLALDQEDSLDVGARAQIPFTAERVNVAVLLLPHMSNFTDF
                     NALAQETDVALRYVATPEAALGADVMILPGSKTTLSDLAYLRERGFQSVLQDHVRRGG
                     ELVGVCGGYQMLGQSIADPDGVETGGHDKGLELLPVTTVLARDKITEQVEGQALHIEG
                     STTLTVRGYLIHVGRTDRAGLRPSFQLVRRKTSLDGSTAIVRDEECLDGAVGHDGLVW
                     GTYVHGLFDQPDFRRAWLNRLRSRKGLPAVSLEESQRVNDTRLNAMDRWADHVDRHLD
                     LAPVLSWLR"
     gene            complement(25020..25754)
                     /locus_tag="PK589_11580"
     CDS             complement(25020..25754)
                     /locus_tag="PK589_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HNJ20289.1"
                     /translation="MTQRHANRISTRHWVAGDTLIVDLGVRMRVLSSAPRGGGLRTTR
                     FILNHQVPSHPPGTDTTGRTWEHPSHYLRRLAETHGVEPDCVGLMTAVPMTQLVTHRE
                     ERDGTWVEGFATVGVTNAVRAGEPVRQVQGEQIGATAGTINLIVVTNGCLAVPAMVGA
                     VQVATESKTGVLRDHAVPSWTGLAGATGTGTDAVVVASALRGRGPWYPYAGTHTEIGS
                     MIGRVVAECLLQGLALAAQWADQRRT"
     gene            complement(25751..26860)
                     /gene="cobD"
                     /locus_tag="PK589_11585"
     CDS             complement(25751..26860)
                     /gene="cobD"
                     /locus_tag="PK589_11585"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HNJ20290.1"
                     /translation="MNRQAHGGDVYAAARELKRNVRHLLDFSASINPLGPSPAAWRAI
                     GRGSELLSHYPDPACRDLRQALATFWDRSPEEFLIGNGSTELIHLLPEALQIRHLLVI
                     GPTFSEYAAAMARVGGQISSIMATRDDGYRPPLEQALDNLDKFRNKGNSMPPVDAVML
                     CNPNSPTGQACDAVMVRKLARRAARLGQWCLVDETFAEYCEQVSILPEDLPARTLVLR
                     SFTKFYGLPGLRVGYAVGNPKVIKQVSAHQPPWSVNMLAQRAAQLALQDRRHSRRSLR
                     FIEQERSRLVQELRRLPGCSTFPSAANFLLVELPARLKATRVVSALRRQGILVRDCSQ
                     VPGLNDRSIRLAVRSRADNDRLLNSLSKIIGNSRV"
     gene            complement(26857..27813)
                     /gene="cbiB"
                     /locus_tag="PK589_11590"
     CDS             complement(26857..27813)
                     /gene="cbiB"
                     /locus_tag="PK589_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HNJ20291.1"
                     /translation="MTGVELAVAAALDVAAGDPRWLPHPVKGMGAVIAWVDDRVRRLC
                     RSEQALQIAGGCLALGLPIAVYAAATWVIAQATAIAPLLGQVVGVGLAYTTLAGRDLF
                     DHVQAVLRELAAGNLAGARQAVAMIVGRDSATLTESGVARATVETIAESTSDGIIAPL
                     VYLSLGGAPLALAYKAVNTLDSMIGHLDARYEHFGWASARLDDVLNWVPARLAGGFIA
                     LAAGLSTGQWHRIHESWYMLHRDGDKHASPNSGQPEAAMAGALAVQLGGTNYYDGVAH
                     EAPSIGDGTADITPQHIRQASRIMIVTYGLGLFFALLSLWVS"
     gene            complement(27810..28583)
                     /gene="cobS"
                     /locus_tag="PK589_11595"
     CDS             complement(27810..28583)
                     /gene="cobS"
                     /locus_tag="PK589_11595"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNJ20292.1"
                     /translation="MTEIAAMTRPFVFAWHFLTAIPISRTHHAPTAQELASSMAWYST
                     VGLAIGGLVALADVGLRSWMAAEVGNALLIVLLVLLTRGLHQDGLADTVDGLAGGRTV
                     EDRLRIMRDPRVGAFGATALSLSLLLRYAGLLALPAPLRLPVLLCMPALGRWAMVSLA
                     WASPSARRDGGLAASFLTHLSWQQVLLSSLVLAVALTMALGGTVAVATMGMGVLLLVL
                     IRQRCLTLLGGVTGDVLGATNEVVEMLFLLLIPALVVAA"
     gene            complement(28576..29631)
                     /gene="cobT"
                     /locus_tag="PK589_11600"
     CDS             complement(28576..29631)
                     /gene="cobT"
                     /locus_tag="PK589_11600"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNJ20293.1"
                     /translation="MMTLDDAMGLIQPVNGDSAANAQRRLDRLTKPLGSLGRLEELAV
                     HYARITGDVKPNVPRGVVFTFAADHGVAMEGVSAYPSEVTPQMVLNFLRGGAGVNVLA
                     RHTGVEVRVVDIGVAYDFGVVPGLIQKKIMRGTNNLLREAAMTRAQATEALQVGIDLA
                     TEAAQEGVGLIGTGEMGIGNTTPSAGITAVMTGRPVAEVTDRGTGIDEAGHARKVSVI
                     EQALNRHRPDKADALDVLAKVGGLEIAGLAGLMLGAAAARVPVVLDGFIAGAAALIAV
                     ALQPLCRDYLIASHRSVERGHQATLECLALKPLLDLDLRLGEGTGACLGMGLVQASIK
                     ILTEMATFDEAGVTERD"
     gene            complement(29641..30198)
                     /gene="cobU"
                     /locus_tag="PK589_11605"
     CDS             complement(29641..30198)
                     /gene="cobU"
                     /locus_tag="PK589_11605"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNJ20294.1"
                     /translation="MPRSSKSTTRPGRAGSRLILVLGGASSGKSQAALERAGSQAPKA
                     FVATGQGLDKEMQARIARHRATRSAEWVTAEVPRDLAGWFRSDGSGYRTIVVDCLTLW
                     LSNVCGRRLAGADMSARVDELVHAIRQSPARVVLVSNELGFGLVPTSRSARAFRDVAG
                     RVNQQIAASADEVYFVISGQSLRLK"
     gene            complement(30182..30778)
                     /locus_tag="PK589_11610"
     CDS             complement(30182..30778)
                     /locus_tag="PK589_11610"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNJ20295.1"
                     /translation="MRITKVYTRTGDAGQTRLAGGQQVWKDCVRVEAYGTVDELNASV
                     GLVRAMNAEGGSASPASAQLETDLRWIQNKLFDVGSLLATAPGQSFPNMPKVTEADVT
                     RLEQMIDRCQEDLAPLKEFILPGGGKVSATLHQARTICRRAERDCIRLSREEDVSAEL
                     NKYLNRLSDALFVMARWVAKTQGEPEFLWQRESDAPIK"
     gene            complement(30843..31046)
                     /locus_tag="PK589_11615"
     CDS             complement(30843..31046)
                     /locus_tag="PK589_11615"
                     /inference="COORDINATES: protein motif:HMM:NF025733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HNJ20296.1"
                     /translation="MSKTCEHCRQPFECVGYQCWCGKIGITEAQMDWIAARYQDCLCP
                     ACLQQVADGKLGPGVPPQADQAR"
     gene            complement(31055..31654)
                     /gene="cobO"
                     /locus_tag="PK589_11620"
     CDS             complement(31055..31654)
                     /gene="cobO"
                     /locus_tag="PK589_11620"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNJ20297.1"
                     /translation="MTDRDEHTAKMQRLKGSVDRRIEAAQEEKGLLMVYTGAGKGKTT
                     AALGMAIRCLGHGMKVAIVQFIKGAIDTAEERILRSFGDRVMFLRMGEGYTWETQDRE
                     RDTRFAQEAWNKACEFMTDPSYAMVILDEFNIALHHHYVEVAEVLPRLQARPLMQHVV
                     ITGRGAPAELIDAADLVTEMKQVKHPFRKGIKAQAGVEF"
     gene            complement(31667..33052)
                     /locus_tag="PK589_11625"
     CDS             complement(31667..33052)
                     /locus_tag="PK589_11625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HNJ20298.1"
                     /translation="MPRPRLVIAGTHSGVGKTTVTLALMAALKARGLVVQPFKAGPDF
                     IDPGHHQLVTGRPSRNLDGWMLNEAVNRSIFSQASADADLSIIEGMMGLFDGSSPIHE
                     QGSTAELAKQLHAPVLLVIDGSAMARSAAAMAAGYARFDPGLSVQGVLFNRVRSDGHY
                     RLLQEAVEAETGLAVVGYLRPDASVTIPDRHLGLRTAIEAGSCDLYDRLAQSAAETID
                     LARVEAIARSAEGWFEEDPVSPPAAGPKETVRVGVAYDGGFCFYYQDNLELLAAAGAE
                     IVQFSPLHDAALPDVDLLYFGGGYPELYGEALAANVSMRAAVKAFSDRGGVIYAECGG
                     LMYLTQAIRDGDGHRYEMVGVFPAEAVMRRSGMTLGYRIVETMRPSLLGPAGTSVRGH
                     EFHYSLLEPKGELAYACVLADAAGKPVGQDGVMVRNTLALYSHQHFRSRPEVVVNLLQ
                     SVRQEKAARQA"
     gene            complement(33077..35059)
                     /locus_tag="PK589_11630"
     CDS             complement(33077..35059)
                     /locus_tag="PK589_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ20299.1"
                     /translation="MLKRVREWSVCCLLLLIVSTPPVWPAVAGAEEPEEPEPVVQAEE
                     VVISATKTPVPVTQVTSAVEVITAQDMKKQNIRHVADALRLAQGLAVFSSGGPGTEVN
                     ARIRGGGSSQTLVLIDGAIVNSGTLGSYDFSNLTTDNIERVEILRGAQSMLWGSDAMG
                     GVINIVTKRGEGPLTATGFMEYGSFATLREGGTVSGKQGLVDYSLSLSRWDTSGFSAV
                     NYRRGATERDSFRNWQGSGRIGVDLPRDGRLDFTMRWMNSDTQLDNVSATFPADVYGS
                     KMRSQQYVFSGSYEQPITGWWSQKLTLARSQEASLFLPGTVQRSLVDGFISTPFGSPN
                     ETRVLSNRLEWQHNFQVTRLLLLSAGYQFREQQGENDTGLINRVLSSHAGFAQAQFNL
                     WDRVFATAGVRHDSYNVFGNATTYRLTGGYYQKETDTKLRVGYSTGFRAPSMNELYFP
                     NFGNPNLGPEKNQSMDAGIDQYFFSKQLKLSGGFFWNHYRNLIVTTFDPGFCAPFSTF
                     GFCPQQLGEASTKGWEAGLTYTYSSDRPFLKGVVVQAQYTNTITRDLDTQTRLPRWPT
                     DQWSASVSYQPIDPLWITVIGRYVGSRFNTTGDRQAMPAFDVWSLAVTYDVTKQMQAY
                     LRAENLFNEKYEEIASAGTPIRSIFGGLRVTFGGKS"
     regulatory      complement(35088..35344)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(35410..>36033)
                     /locus_tag="PK589_11635"
     CDS             complement(35410..>36033)
                     /locus_tag="PK589_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ20300.1"
                     /translation="QSLAGLSQDDTARAVACVPQENPQLFPFTVAETVLMGRFPHRHR
                     SRWSLGFGWEDQDDCRAAAQAMETMDITHLASRLVTDLSGGERQRTMIARALAQMPRV
                     LLLDEPTAFLDLQHQLDICSVVRRLREERGLTVVIVSHDLNLASQYCDRIAMLKDGRV
                     AAIGTPSEVMSVETLREVYGCTVLIDAHPDSGLPRITLPGPAVPVHR"
BASE COUNT         7138 a        11357 c        10144 g         7194 t
ORIGIN      
        1 gcgatccgct aagatgctcg tcagtccggc gaggcgttca tggctgcccc taccttatat
       61 atctcccgtc tgctcccgga tccggtgatg accgccgccc ggcggcaatt ccaactgttg
      121 aacgatcctc gtgatgcctc gccctcttgg gacaatgtga aaacaggcct acgtgaggcc
      181 gacgccgcga tctgcacgct caccgaccgc gtggatgcct ccatgctggc cgaggcgacg
      241 cgtctcaaaa tcctggcgaa ttatgccgtg ggctacaaca acatcgatct cgctgccgcg
      301 aaggcgcgcg gcattgtggt gaccaacaca ccggatgtgt tgaccgactc tacggcggat
      361 ctgacctggg cgttacttct ggcggtgacg cgacgggtgg cggagggcga tgcctacgtc
      421 cgatccggag attggtccgg ttgggcgccg acgcagatgc tgggcacgga cgtatcggga
      481 aaaaccttgg gcatcgtcgg gatgggacgc atcgggcagg ccgtggcgca gcgggccgcc
      541 ggtttcaaca tgcgtatcct gtatacctca cgcaccccga cggctctcga acgagtgtcg
      601 tctcaatggg aatcccgcac cctccccgat ctgctgaagg aagccgattt tgtgagtgtg
      661 catgtgccgc tgactgccgc cacccatcac ctgatcggtg cccgccaact cgcgctgatg
      721 aaaccgaccg cgttcctgat caatacatca cgtgggccgg tggtcgagga ggccgcgctg
      781 gttgatgcgc tccttcagcg caggctggcc ggtgcggggc tcgatgtctt cgagcatgaa
      841 ccggcctttc atccgagcct gcgcgaactc aggcaggtcg tgctgctgcc ccatttggga
      901 tccgccactc tggccaccag ggtccgcatg ggcatgatct gtctggaaaa catcgcggca
      961 gtggttgcgg gaaagccggc gccgaatcag gtccacgccg cctaacacag ctccacgccg
     1021 tgacgctcga gacgagagca agactgtaga aggactggcc ttagtggccc gcgatgggca
     1081 gaggactggt cggagtggat gcgggaagtt ccggatgcgc ggccccgttg gtcgactgca
     1141 tccgcagcgc ttcaacccgg cgaggcacgt cgtggaacga cggaatggac tgatacatgg
     1201 tgagcgcccg atcgcgagcg cccgtcatct cgtagagtaa cgcgagttcg taccggacca
     1261 attgcgcgtt gcctccgcgg cagcgcgagt cagagatcag tttctccaac aactgaatgg
     1321 cctgatccgg acggctctgc tccttgcagc agagggccat catcaggcag gagtccacaa
     1381 agaatccgga atccttcatc gagaggatga actcttcctt cgcttcctcg cagagcccca
     1441 tgtctttgta ggccatgccc agggcataac gcgtttccgc atctatcggc tccacgacgg
     1501 gtgcaggcgg tgccaccggc ggtggcgcct ggggaggagg cgcggcggca atgaccggtt
     1561 caacttcgac gacaggtgcg ggagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnntgggga ggaggcgcgg cggcaatgac cggttcaact tcgacgacag gtgcgggagg
     1741 cacctcaggc ggcggagcag cctgtacgac gaccggctcg accacgggtt cgacattcgg
     1801 cggtttcacg actgcagctt cgagcggcgg cgcagggtca ccgacaaatc taaacggcac
     1861 ctcggcgggt ggtgctgcgg ccatgatcac cggcgtcggt tccaattctg gcgccggctc
     1921 gacaacaggc tcaggcgccg gcgttaggac gacttccatc accggcgcaa tgggttccgg
     1981 ttccggcatc gacccggcat taggatcgaa cgcaggcgcg agcttccggg ctatcgggct
     2041 ccctggcgcc agggcctgca ctttttcgaa gagctcagcc ggcaaggtcg gcatgcccgg
     2101 ctcaggatgc tccaacaaaa tctccacggc ccggccgaaa tgctgcgccg ccgtggcagc
     2161 atcacccttc tcctgataga gctcgcccag tagttccaac atggggacgg aggccggttc
     2221 cactttgaga tagtctgcaa tcaacgactc ggccacgcca tagtcctgcg cgcgcaaggc
     2281 cgcaccggcc agaaatcgat attcgcccag ggccacgacc agattaccct gtaagagatg
     2341 catccgggcc agtaattggc agatctccgg attgccgggt tcgcggctca gcaattgggt
     2401 caacatggcc tcggcgccgg catactgccc ttcttcgata cggcgcgtgg cttcagccat
     2461 gagatcgctc gcatcgccgg gcttggctgc ggtggtagcc ggtttggccg ggcgtgccgc
     2521 actgggcgtc acactgccgc cggtttcgat catcgacgtg atttctttgg cctcggcatt
     2581 ctccggatcc aggcgcagca ccgccgtata catcgctttc gcctgctcgt actgctgcgc
     2641 ggccgactgc tcgcggccaa gttgcagata catccgcacc gcctcgtcga cgagcccttc
     2701 ctgcacacag agctccgcga cgcgtaactg ggcgtccaga ttagacggat cctgggagac
     2761 gatcttgcgg taaatctcga gcgcttcttt gttccggccg tccttgaagt aatgtttgcc
     2821 cagcgtgaga tagtcctgta cggcgctgct cagcagcccc cgttcggcat tcagatcacc
     2881 gagatggcga tagatttcga tccgggccgg atcgaccttg agtattttct tataggcggc
     2941 gatagccttg aggacggaac cttcggagcg gaatgcgctg gcggcttgca gaaacgccgt
     3001 gacggcctcg gcggtggcgt tgcgcttgag ttgaagatcg ccgatggagt taaacaccgt
     3061 gccgtcgtta ggcgtgtcgg cgatcagttt ccgccattcg gcaatggcgg catcgtactg
     3121 ccctttggac gcaaagagct gcgcgttctg tagcactgta cggcggtcga cagccaagga
     3181 gacccctcac ccgacgttga gagtccacgc taacagtcaa cctatttatt gtcaaaacaa
     3241 atggataaaa gagagacacc gcacacgcgg cgtccggagc ggggattctc tgaaggaaac
     3301 cggcggcccc tcgatgaggc agaggggccg cggattacgt atgactccgg ccgaaattag
     3361 ttggccggcg cgagatcctt caacacgttg gccgcctgcg gtcccttggc gccctgaacg
     3421 atgtcgaact ccacgttctc gccctctttc aaggtcttga acccgtcgcc ctgcagcgca
     3481 gagtaatgca cgaagacgtc gtctccgcca tcgagccgga taaatccgaa tcccttacga
     3541 tcgttgaacc acttcacggt gcccttagtc ttcatacggt cctccttttc tcaacgcacg
     3601 gacataatga gcgcatcagc tcgtctgctg agtcgcatga taggaaatga tgacgataaa
     3661 aaaggagaaa caacagcgac gagcgcggcg gcttttgcgg gggcgttcag acaatattca
     3721 acccgaacca tcttaacctg ctgaaaagtg cgcagcgatg taccatgggg tgaaatgctt
     3781 gtcaagcgga tctttatggc cctggcctat ctgatctgtt tacaaggcac gcagactctt
     3841 ctatagtgca gcatccaatt ctccgatgta ggaccccgac cgctctcgtg ctgcacacca
     3901 tcctcgatcg ttacatcttc cgcgagttgc tttcgccgtt ttgcatcagc ttaggcgcgc
     3961 tgtgcttcgt gatgctcacg aaggaactgc tccgcctggt ggagctgctg gtgacgaagg
     4021 gcatcggctt tctgtcggtg ttaaaagtct tcgcccactt actcccctcg ttcctcgtgc
     4081 tcaccctgcc catcgccggc atcattgcct cgattacggc cttcggccgg ctctcgctgg
     4141 ataaagaact cgtcgccatg cgcgcggccg gcttcagcct gttgcggctc tcccagccgg
     4201 tgtttttgtt tgcggcgctc gtctgcggcc tcacgctcat gatggcgcaa tacgggcagc
     4261 cgtggagcaa tgtcaatctc aagaaagtcg cgctgaacct gttacgcgac gaactggtgt
     4321 tggagctcga ccgcggcgtg ttcaacgaag cgatccctaa gatggtgatc tacgtaccgg
     4381 atgctcagga ggggcaggac aaccggggca ttttcgtggc ggatgaacgg aacccggctg
     4441 atccgcgcat catcgtggcg cagcagtacc aggtgatgac cgactcggca agcagccagg
     4501 tggcgctgcg cctcatgaac ggcgtcatcc acagccgtcc gcaaaacccc gaggagtatc
     4561 agaagatttc gtttacggcc tacgatctca aattgagcat caacgccagc ctgtacggag
     4621 ccgaagagcg cacgccgatg gacgtgattc gcgcgaaact ggaaagttcc aactggacgg
     4681 acaccaatgc cttgcggcgg ctcatggaat attataaaga cctggcgttt ccggccgcgt
     4741 cgctggtgtt ctgcatcctg ggcgtgccgg tcgggatcgt gtcgaaacgc tccggcagca
     4801 tcggcgggtt tgccgtcggc gtgctcgtcg tcatcgccta ctacgtcatc aatgtcgcct
     4861 gcgagtttct cgtcaccacg ctgtggatct ccccgttcgc cggcgcatgg ttacccaatg
     4921 tcatcttcac gatcgtgacg attttgtggt tttatcgcgt gagccggcag taaccccatg
     4981 aacattcttt ttcgatacat gctgcgcgag tacgtgaaga ttttcggcat gtgcttctcc
     5041 gggctcatga cgatttatct cgtcatcgac ttttttgaaa aggtgcgccg cttcctccgg
     5101 tatgacgcgc atgcgctcga tgtgctggcc tacttcgtcc tgaagatgcc ggccatctcc
     5161 tatcagattg ctccgctggc ggtcttgatg gcgaccctct tgaccatcgg cctgctgtcg
     5221 cgcagccacg aaattacggc catgcgcagc tgcggcatca gcctgtactg gatcacctcg
     5281 ccgtttattt ttctcggaac ggtcctggcg atggtgttgt tcctgttcag ttccaccgtc
     5341 atccccctgg ccctggcgaa ggccgaccag atcaagacga cacagatcga gaagcgcgtc
     5401 acgcccgtct ccttgaaggc ggcgcagcca tgggtcagtc tggggggaca gaccctgttg
     5461 aacatcgaca ccatcgatcc cggaggcgcg gtgttgcgca acatccggct ctatcgcttg
     5521 agtccgacct ttcaattgga ggaaatcgcg gaagccaaga gtgcggtcta ttcctcgcag
     5581 ggatgggcct tgcatgacgg catgacgcgt tcattccgcc aggacggcac ggtgctggtg
     5641 agcagctttc agacgcaacc gctggcgctg ccccacattc ccgacgactt taccacctgg
     5701 gccgaactgg actcggaaac gatgaccttg cgcgagattc gcgcctatgt cgatcgcctc
     5761 cacctgggcg gcaccatgct cccccgcttg ttgacggatt attacggccg gatcgccttc
     5821 ccctgcgtga cgctgatcat ggtgatcgtc ggcattgcct tgagccttcg ccgcacgggt
     5881 gtccgcggaa gcggcatggc gatgggcatc gggcaagcga tggcggtcgg gttcttctat
     5941 tggtccacgc actccgtggc cattgcgctg gggcgcggcg gtgcgttgtc tccgatgctg
     6001 gccggatgga tggcgaatat ggtgtttctg acgttcggtt tgtatctgct catgaaggtg
     6061 cgctactaaa aggatgttga acgtgtccgc cagcgtcgtt ctcggatcat acgaaccatc
     6121 gatctaattg gctggaacgc ctgcgggcct tcacgtgctg cagtcttgct ggcgccaatt
     6181 tgagcctcct gtgcggtggt tccaccacga tacctacgga gatctcgagc gggtgcctgc
     6241 gtacagcatt gactgtgacg cccccgtgcg gtaagtagac aggccgcaca cctcaccatc
     6301 ctgcggccat gaaagggttt tatgtcgacg agggtcgtca ttacagggct gggagtcgtc
     6361 tcccccatcg gcatcggcgt gccgcaattc tggaaagcgg cactggaagg ccgatcgggg
     6421 atttccgcca ttccctcctt cgatcccttt ccccttgaag gctatcggtc gcgcatcgcg
     6481 gggcgcatcg tcgacttctc cccggaccag tatctccctg ccggacaggg cgatcgtgtg
     6541 gatcgatatg cgcaatttgc gctcgtggcc gctaaggaag cgctcgctga tgcggacttc
     6601 aagatggaac gtgaaaaccc gcaccgggtg ggtgtgatcg tcggagccgg catgggcggg
     6661 atggtgatgg gcgaacgtga gatcacacaa ctctatgaac agaagcgccc gcatcgggtg
     6721 catccgaact ttatcccggt gatcacactg aattccgcct ccggcatcgt ggccatggcc
     6781 tatggagcca agggccccaa cctcacgatt tccactgcct gctcatcgag tgcccacgcg
     6841 ttgggacagg ccatgcacgc cattcgcgcc ggcacggccg acgccgtgat cgtcgtcgga
     6901 gccgatgcca gcattacgcc tctcgtcttc gcaggatttt gctcgcttcg agccctctcc
     6961 acgaagtata acgatgcccc ggagaaagcc tcgcgcccct ttgaccgtgg acgggacggg
     7021 ttcgtgatgg gggaaggcgc cggcgccctg atcctcgaat cattggccca tgcgaagaaa
     7081 cgcaaggccc ggatgtacgc ggaagtagcc ggatatgccg ccacgagcga ggcccatcac
     7141 atggtcatcc cccgggagga cggcgaggag gtcgccacga ccatgcggct ggccctcaag
     7201 gatgcgggcg tgacgccggc gcaggtggac tatatcaacg cgcacgccac gtcgaccacg
     7261 gtcggcgacg ccgtggaggt taaggccatt cgcttagtat tcaagtcacg cgcggataaa
     7321 ctcgccgtga acgccacgaa gtctctggtg ggccacacac tcggcgcggc cgggtcgctg
     7381 gccggagtgg tctccgccct cacgctcacg agcgggcaga ttcatcccac gctcaacctg
     7441 gacgatcccg acccggcctg cgcactggcc ggcctgtcga aggatcagca aacacgcaag
     7501 gcgaaagtgg cgctgatcaa cgcatttgga ttcggcagca acaacgcggc cgtggtgtta
     7561 aaagctgtct cctcctgacg cctgtccggc aggacagttt tgacggccga cgaaccagac
     7621 acgacacgaa ggaaccgggc atggccacta ccacagacgt ttccagcaag atcattcagg
     7681 cgttggcaga gtatctgaag cgggatgcat cctcgattac gccgacgcat cacttgcggg
     7741 aagatcttgg cctggactcg atggcggtca ttgaaatgct gtatcgcatc gaagaagtgt
     7801 tcaacctgca gatccccgac caggatctgg tcggcctcac caccgtcggc catgtcatcg
     7861 cctacgtgga aggacgcgtg gcaccggcct cgacaaagac cgctgccaag ggcgctgcga
     7921 agccctctgc aaagactccg gccaagcccg ctgccaagaa gcctgccgcg aaaccttcgc
     7981 ccgagagtaa gaaagcctaa cagaatgagc acggcaccca ggcgcacccc gctgacactt
     8041 cgggaactgc acgactatgt aggaggcgaa ctgatcggtt ctccggacgc gaccgtcacc
     8101 gccgtggcca gcctggagca ggcaggacca ggcgatctcg cctttgtcac atccgagcgc
     8161 catctcaagg ctccacagac cgccactttc ggcgcgctat tggtcggacg gcgcatcgcg
     8221 gattgcccct ccccgcaaat tgtggtggag aaccccgcct acgcctttgc gcgtgcggct
     8281 caacagtttt tcgcgcgacc cgcccgagtg cgtggcatcg cggagtgcct cacacgcggt
     8341 gacggcgtca cgatcgggac cgatgtttcc atttggcctg gcgtcacctt aggcgaccgg
     8401 gtgaccatcg gcgcgcgagt gaccctgtat cccggagtct tcatcgggga cgacaccagc
     8461 atcggcgacg acagcctcct ctacccgaat gtggtcgtcc gggaaggatg ccggatcggc
     8521 gcccgcgtca tcattcacag cggcacggtg atcggcagcg acggcttcgg gtatgtccaa
     8581 taccagggcc gacaccagaa aatccctcag ctgggcggcg tgctgatcga agacgacgtg
     8641 gaattgggtg ccaatgtctc ggtggatcgc gcgacgtttg gagacactgt catcaaacgc
     8701 gggaccaaga tcgacaacct cgtgcagatc gctcacaacg tggtggtcgg cgaacacaac
     8761 attttagtgg cacaggtcgg tattgcgggc agcaccaccc tcggacggta cgtgatggtg
     8821 ggcggtcagg cgggcctcgc cgatcatttg cagatcggcg atcaggtcat gatcgcggca
     8881 aaatccggcg tgaccagaag tttggagccg aatcagatcg tctccggcgc accggtgatg
     8941 gcccacgcca catttctaaa agcccaggcg gtcattcctc agctgccgga actgcgcaac
     9001 cgggtccgcg aactcgagga acgtctcgca actctggagc aagccgccaa gcccgccgcc
     9061 aaaacccgca agcctcgccg taagtagctc gcacaccgat atcgtcaggg tttgagcagc
     9121 gtcttccaga aaaacagctt ggcccagaag aatccggccc acggcaggaa gcacgccgga
     9181 atgggatgga ctgcgccata gagcatgctg agcatcgatc cggcaagcag gaccgccagg
     9241 ccgaccatgt aggcacctgg agcccatggc gagccactgg atgactcctg attcttcccg
     9301 ctctcccgtt taggtttccc gcttttcgtg agcgcgggct gccgcatgcg ctcggcaaag
     9361 acctcaggac gattggccag aatccagcga tggtacgtgg tctgcaccca ccccaagagc
     9421 ccgcccagca gcaacaagcc tgagctttcg acaaaggtgg tccacgaata ggtgtccgtt
     9481 gcgatgagtt ggtacgccag cccgccgacg ccgccgagca tggaaatcag gccgagaatg
     9541 ccggccggaa agagcatgta cgctttgata taggccgtcg cgcgcgcgtc gtgcccttcc
     9601 tgttgtttga cggagacaag ttttggcatg gccgtgatgg tcgtataatc cccacctcaa
     9661 ccgcaagaga aattgtacag attgacgaga tatgttttgt gtgcgcccca cgtgagcggc
     9721 atcacacggc ctgtccacaa gaactgtgga cggactgtgc gcaaccatgt ggatggaacg
     9781 cttttcgtcg gactacgagg gggcgcagat caactgccta aggattaggc gggcagaatc
     9841 ggtgagcaga gagaaccagg gtccgtcagt ggaccacgac gctaggccat cggcagaata
     9901 tcaaaaatgg ccgcccgttc gacccggccg acatccaccc attgctgggc caacggaata
     9961 agcgtcgcca agcgctcttc cactgcagcc agccgctcgc gaataacggc cggcttatgt
    10021 tgcgcggtca ccctgagaaa tgttcgcaga ccttgcagaa agacactgat attttccgcg
    10081 gaaacctttt cggacgcctc gaacagcaca tcaatttctt caaaaatcgg ctcgagagcc
    10141 tgagggggca acacgcgttc ttcgctcgcc cgagcaatac ggccgagcgt cgagaccact
    10201 gaaggaaccc gagcctgcat gtgattgaga aatcgttcat ccaactcatc caattcctgc
    10261 agcaggcgac cgatggcccg tgcatccacc acgggcgcgc cctgagccag ctcgcgttcg
    10321 gctcgttgaa tgaccgtctc caacacatcg cgcactggct ccagcgagcg tccgcgtgcg
    10381 acatgcaatt gtcgcaaggc atcgagaatg gatgacgccg gcatggggcg cggaggagcg
    10441 taccggggcg tagcgatttc agaggcgggt tcgctggagg cctgcgactc cggcaactcc
    10501 gtccgcgccg gttgttcaag gatcggctgt tctcgctcaa cagtctcctc ggctggcgca
    10561 ggatgttctt caatcggtcg aagctcctgt accgtcttgc tgatctccag caacccttct
    10621 cgaagggatg ccacgtgatg cgcggcaacg gcccgttcat gcctggcagc cgcctgcagg
    10681 agtggaaaga gcctggtcgc catcgtttcc acggccgtca atccgaccgt ggccgcggaa
    10741 cgggcgaggt tgttcacgct ttgccataaa atggaggcca attgtgcgcg gcgcgcctgc
    10801 tcactggtgg catccagacg ttccagcgcc gtttgaatct gattgagcca ttcccgcgct
    10861 tcaaggacga acaggcctaa gatttcccgg cgtacaggga tcaacggttc tgccgtctcc
    10921 gcctcagccg gtacctgggc cggtcccctg gctacggctg gctggtccac ggcagcctgg
    10981 atgaccccgg caatggcgtc gagactttcc agcagggccg caaattggtc tgagacgttt
    11041 gttggcgcag cagatgctgc tggtgcagcc ccggccgctt cgaatgtgac acgatccaac
    11101 gccggcggct cgacggtatc caccactgac acatggggca agagactcaa atcaagcagg
    11161 tgcgggggtt ctgaaggcgc cgcagtctct gtgctcgtga tgcgcaagac cggccgcgag
    11221 gggtaccggg ccgtgattcg taccaccacc ccttcttgtc cgctgctgag ccgcaccctc
    11281 gtgccgagtg gatacaccga caactgttcg accaacgctt tcatgatttc ccggggaaac
    11341 gccgtgcgtt ccgtcgtcag caattcgcgc acggcttcgt gcggcagcaa tcgtcgccga
    11401 tagggacgtg gactcaccag ggcatcaaag atgtccacca agccgatgat ttgtgccagc
    11461 tcgttgatct ctcgaccttt caagcgatgg ggatacccct gccctttccc ccgctcatgc
    11521 gcttgcagca cgacttctgc caaccatcca tactccgtcc ccacacgtct gatgacttcg
    11581 gaaccgagac gtggatgttg ctcgaccaac gtccgttctt cggctgtcag gcgcccggcc
    11641 ttcgtgagga gctgctgcgg tacggcgaaa attccgatgt cgtgcagcaa tccggctacg
    11701 gcgaggcggt gtaactcgac accgtagtac ccaagaccga tcccaacctt tgtcgccaga
    11761 atgccgacat tgatgaggtt ggtgactagc ggaggcccgc tgggactgga gagcgcttcc
    11821 acgaccagtt gatcgctgcc ctgtaacgat tcgacgatac tgtctgcgag atgcgtgagc
    11881 tcggtgagat ctacgtcttc ctgccgttgg acggaggcag ccaccttcgc caggatctcc
    11941 tctgcgtgcc ggtagtggtt ctgttccata cttctttctt caactccccg ccttggctgg
    12001 ctaaatgtgg ccgtcgggag tcgcaatcgc gacggatctc actcgaatag attcagagtc
    12061 attgtgacat cgagcctgtg gtgagcatga tcgccgattc aaccggtccg ctgcggaagg
    12121 tttgcgacgg cctgccggtc gcttcgcacc gttaggtcga gggggcgagc cggctcatca
    12181 tgtcgaactc tttttgcccg tccgcaatcc actcttcgac catgctggcg acggccagaa
    12241 tgcgggtctc gaccgattgg agccgatgag ccgcaatatc gatgcggcgt tggacgacca
    12301 atgtgaggaa attctgcagg ccggtcagga acgtgacgag cggcgtggca ttcgtctgct
    12361 tggcaatctc gtgaagactt gcaatcatct gtattggagc atcgaaaccg gcatcggagg
    12421 tcaggacggc aaccccgccg gctcggagcc ggctgaggtg cagcgcaatg ccaggcaagt
    12481 cctggcggag cgacccgaga tattgggcgt cggtattgtg aatcgtcagt aacagctcct
    12541 gaaactgtgc agcggtaatc tggccagcgc ctatacgcgc ttcgtgttcc aaacgctgca
    12601 gcacctgcgg gatgaggctg cgggacatgc tgccttgcgc ggcctgttca tcctgcagct
    12661 ggtgcagtgc attcaacagg ggaagaaact ccaacaccgg ttcaggcgcc ggcgccgcct
    12721 ccggcaaggg atcaatgtcg agcgtgattc cggtggccac tttcacggac gcgatgacgg
    12781 tccgaaaatg ttctcggacc gtgctgaagt cctgtttggt tgcggtggtc cggtctttga
    12841 tcgtctcaat gaacggaagg agggcaaacg tggcgcgttc gacatcgggc agactcaccg
    12901 tggcagctga gccgccgagg ctggtaatgc cccgcactac cgcgtcgatt aattgggaat
    12961 gccgatccga atccggcagg ctctcgagtt cgaccagggc cgcgtggatc tgcacaagcc
    13021 actcctgcgc ctcctgcccg aacagctcaa tgagctcttt ctggaaatcg tcttgggttt
    13081 gctctggttc cgtcgacacc gtggatcctg cctctgacgc atgcacccgc ctgctaccgg
    13141 acgagtgacg gcctcttagc gtttggcatt tccgagttgg gaagcgaggc ctgcgatttc
    13201 gcccagtttt cgcgccagat cttcaaatcg tcgcgtagac tgttcagcca tcgactcggc
    13261 atcagcgact tgttttgtga gttgcatgga gtggtcccgc tcggcactca gctcctgctc
    13321 caagtccggc accagctgag ccttctcttc catctgcttg tattgcgcca gaagcgcagc
    13381 tgcagcctca cgttcagcgg tgagtgttct ttcaagttct gcaacctgag cgcgagcgtg
    13441 ttcacccgcc tcttccaata ccggcaccat ggccgcactc ttttccaaat gcgcccgttc
    13501 gttccgcaga gccatcgcct cttcccgctc gctcgccagc acccgctcga gctcccgcat
    13561 gcggtcttcc acgtgctcaa ggctggtaat ctgattgacc agttgattgg ccgcttcacg
    13621 ctcggcttcg agatgcgctt ccaactcaac gatgcgcgca ccaagacggg cgctctccgc
    13681 cgcttgctgc gccagctggt cggcgcgatg ccgttcttcg agcaacgcgg cttccacctc
    13741 acccaaccga tgcgcctgcc tggacagatc ttccagctgt tgcgtcatcg actcccgttg
    13801 ggctcgctct tttgcaaggt cctgctcgag ggcttgcatc cgggaggaca catccgccat
    13861 ctgttcgcgt tgctccgaca ctgtcagctg atgctgctct cgctcctcct gactgtgtcg
    13921 acgctccgct cccagctgtg cttccagttc ggcaattttg gcatcccgat cggccagcag
    13981 cgcctggtgc cgatcatcag cctgcatcac agggctcggc tcagcagcct gatggattga
    14041 aatgctcccc gcgcgacttt cctcgatccc tacaggtccg gccggcgttt ggtccagaac
    14101 gatggccacc ggttcagccg gctgttgcca ctcacgctgg actaacggga ttgacggaag
    14161 ctcttcctgc gcaggtgcga cgggcgctgg cgcaacctca gccatgaccg gaggctccgc
    14221 aatcggaatg cgaatcggtt cagccaccgg ggccggcttg tgcggtcgct tggtcagcaa
    14281 atccaacacg cgatctttca gagaagatcc ctgaaacggc ttcttcaaca caccgtcggc
    14341 cttgcaggac tccgcctgtt tggacacctc gtcgttcaca atgccgctga tcagtagtac
    14401 ggggagatcg gccaggttgg cttgcccgcg gatgaacgcg cacacttcgt agccactctt
    14461 gtcgggcatg atgacatcgg agacgatcag atccggacga tcttttgcca gcatcgccag
    14521 ggcttctgat ccgttggccg ccagcgtgac gcccattccg gcctcagtca aaagtcgttc
    14581 ggctaccttg cgcaccgcaa tgctgtcatc ggcaatcaag atctttggca tgtcattcct
    14641 ctacgcgatg tcggatgcat caagccggac gctgtgatgc gacccctaaa gttgccaggt
    14701 tgatgagcgg cagttgttcg tcgcctgcga cacaggatcc ggcgatgtcc ggatcgaggc
    14761 cgctttgata ctgtacccct gagcgcggca ccacatgcag cgatggcacc tgagaatcga
    14821 tgcaaatggc aagaggaccg tcgacatgtt tgacgatcag acagagcgac tcctcttcgc
    14881 gcaccgtacg gttgaatttt gctgcaagat cgaagaccgg caggcacgat tcctcatggc
    14941 ggaccagcga ggtgacaagc ggcgtatcac tgggaacagc ggtcgttcca ggccaaggga
    15001 ggacaccgcc gatctcttcg gtcctggcag ccagccgttt tcccgccacg gaaaacacca
    15061 ccatattttg cacgcgcgca tgggcagcac ctgaggccgt cgtgtgggga tgtctgcgta
    15121 attggctacg tagcatgatg accctcgacg cttaactcac agggctcgtc gacatcgaca
    15181 tcgcctcatc cacacccacc atcggcacat gcagccggga atcactctcg ccggccttgc
    15241 tctccgcaaa tcgaatcgca aacatctcca gagtttgcgc cctgagcgga agctcaagca
    15301 cccacaatgg attggcaatg agcgcgacaa aggaggcgcc gacaaaaaat cccagcaatc
    15361 gctcccgttc cttgcctcga aactgaggcg gcaagggatg aatcagcgct cgctcgacgt
    15421 ccagcaatcc cacaacttta tcaaccgcaa aggatcgcga tcgttcgtca ttgccataca
    15481 gaatcagcct ggtgtcggag gacacacctt tggagaccac cttgagtcga gcggacagat
    15541 cggttcgttc gtactccacg ccggcccacg tgaccagccc gttagggccg gccgaagctg
    15601 gcatcacgat gccgcgaacc cactgtgcag gaaaggcgac ggatgagcca ccgatcagcg
    15661 tgacaagaaa tcgaaccgct tgcccgctcg aaatggtgac tgcccgttgt cctcgaagcc
    15721 ccatatgtgt ccttcattct gccagcaacg gcacaagtcg gcaaaacctt gctatttttc
    15781 ggcccgcgcc tcaacaccgg tccacttgcg cacttcctga atcaacgccc gttcttcaac
    15841 cggtttggca atgtagcccg atgccccgac actgaccgcc atctggcgat gtttttcacc
    15901 cgcccgcgtc gtcatcacga gaatgggagt ggcatgagtt tgaggtctcg cccggagggc
    15961 ttggatgacc tcatacccgt tcaacttggg catctcgagg tcggtgataa tcagttggta
    16021 ggcctgcacc atcgccttgc ggcaaccttc ttcgccgtcc gtcgcggtct ccacggtata
    16081 ccccgcggct tcgagcatgc ggcccacaaa ctttctgatg ctgagcgaat cgtcaatcag
    16141 tagcaacggt aacgccgagg gagcactcgc ttttggctca ctatgccctg tagcatcctc
    16201 aatcgctgcg accgaagaat cctgtgcgat cgacatagtt tcctggtact cacgcgtggt
    16261 caggcggctg acgtccagca ccagcaccac ccgtccctca ggatcgatcg tcgcgccacc
    16321 gaacacggag cgttcatacg gcttcagtcc accgagagac ttaatgacga tttcctgccg
    16381 gcccagcaac tcgtcaactg cacaaccgag cgcgccggtc gaggttcgca caatcaccac
    16441 cggagacaca ccgtctgtga tccccgcctc gcgtcgaatg aggctgccca gcggatagac
    16501 ttcgatggcc tcgtccccga tttgaaccac ctgtcgatca cccatgtgtt gaagcgttga
    16561 cgccgtcgac atggtgactt cacgcacact cggcagcgga atcgcatagc ggtcttttcc
    16621 gacccgcacg agcaaggccg tggcgatcaa cagcgtgagc ggcaaatgca tggtgaactt
    16681 ggtgccctgt ccgcgcaccg actccacttc gatatggccg ttcatcgtct cgatgacgcg
    16741 tttgaccaca tccatcccga cgccacgtcc cgcttggtcg ccgacggcgt cagcggtgga
    16801 gaatcctggc aggaagatga atttgatgac ttcgctctcg ggaagggttt cagcgacatc
    16861 ctgcctgacg agtcccaact tgacggcctt cgccttgatc ttggcgatgt cgagacccgc
    16921 gccgtcatct tcgacctcga tcagaaccga gttgccgcga tgcgcggcgt gcaggtaaat
    16981 cgtgccggtc tccggcttgc cttggacacg gcggacggcg gccggttcaa tgccgtggta
    17041 cacggcattt ctgactaaat gcaccagcgg gtccaccaac cgctccacga cgcccgtgtc
    17101 gatttcggta tgttcaccgg aagtgaccag attcacgtct tttcccgtag ccctagccat
    17161 ttcgcgcgcc gcacgccgaa aacgggtaaa cggagtgccg atgggcacca tacgggcacg
    17221 ggcgatttca tcgcgcatcc cgagcgtcaa ttgctgcagc gatcccatgt cgtcctgggc
    17281 gcgatggatt gatcctgaca actgcgacat cgattcgctg atgtctgccg tcacctcgct
    17341 gatgcgacga gccagaatat tgaagtcatc gtacttatcg aattccagac tgccgaaatc
    17401 gctgacaccg ggaaagcctt gaggcatcgc ttctccctgg gtggtgggag atggctgaaa
    17461 cgagaaggtg tgtttgtctt cgaacgtgcg tacggcgtcg gtgagtcgat tcttattggc
    17521 caacacttgc gtagccaatt gatccagggt gcgcaagcgc tgttcgagac gaccgcgatc
    17581 aatcaccaat tcgccgacca gattgaggag ccgttccaag cgatcacggc tgacccggat
    17641 cacctcacgc tcttctccgg ccttcccctc agcggcattc accggctcag cttgttcagc
    17701 ctcctgcaca tcaagcgcaa tcaccgcttc ggtctgcgat gacatgacag gctcatggac
    17761 tcctgccggt gcagcttcac ctagcccctt cagttcttgt agcgaggcgg caaaccgctg
    17821 ccgcgtccga gtgaccaacg acgggtcgcg ccgcatcagg gctcgaatga cgtcgatggc
    17881 tcgcaggagg acatccgtat ggccgggaag aaacttcagg cggccctcgc gaaccgcacc
    17941 catgaaatct tcgatgtggt gtgtgaggtc gccgattgat tggaacccca cggtataggc
    18001 tgaccccttt aacgtatggg ccgtgcgaaa cagttgatgg atgatgtcag ggtttgccgc
    18061 atccttgtcc actcgcaaca agtcggtctc gagactttcc agatattcct gcgcctcggg
    18121 agcgaaatag gagatgactt cggcatccag catcggaatg agatacgcct ccgacaactc
    18181 ggccgggtga ttcggctctg aggactgggc tgccggattt agaggcttcg cggcaggagg
    18241 gagtgcttcc tcgccagcaa cactgctgct cgatgcggcg ggcgcatcaa cctgtggagc
    18301 ttgggtcatg ccttgctcga gctcgcgaag catcgtcgtc atacaaggca cgtcctgccg
    18361 caaccgtggt agctgctgcg cgtctcgcgc catcaacgag cgcatcacgt cgaccgcatg
    18421 ccgaatcgtc gcaatccact gtggtccaat gaccgccgct ccctcacgaa ccgcgatcat
    18481 gcaggtttcg atcgggaatg ccacatcacc caccacctgg aatccgaccg tgtaggcgga
    18541 gcctttgagt gtatgggcga cacgatacag ctggtggatc gtctccgcgt cagacaggtc
    18601 gagctctaac cgctggagaa gcgtctgaat cgtattgaga tactcttccg cttcaggggc
    18661 gaagtacgat agaacttctt cgtccatcac aggcaagaga taatcgtcag ccggctcggt
    18721 cgcaatgggc aggcactggg aggagtcgtc tgcagacgcg gccggcagga attgcgcgca
    18781 ccgttgcacg aacccttcaa cgagagacgc atcttcgcct tcgccccggc cgatccgtgt
    18841 cacctgatgg cggaaggagg cgacgatatc gcggatcact tcaaaggccg ccggccactg
    18901 actcggagac atttcctgca catgttccag cgtctcttcg agtaacgcgc cgagttgccc
    18961 cagaccggga aacccataca aaagtgccgc acctttgagc ttatgaccga cggcatggaa
    19021 ctcggccacc tcggccggtt cgggacgccc cttcccttcc gggtgcaagg ctttccagaa
    19081 ccgttccata tcgtcgccgg cttcggccac gaagatgtcc aacagctggt cgcgatcaaa
    19141 atcagcgctc atagccctac ttcatatccg agcggtgagt aggcgccgct cttctagaat
    19201 tctgctacgc cagcttgaac tgagcgactg aggagttcag gccttcggcc aacttggcca
    19261 tatcctcgat cgtgagtcgg gtggagtcgg tttgtttctg cgtcgccacg gcgccgccgg
    19321 tgaactcctt aatcgcgcga ccgacttttt ccgtcgatgt ggtctgctct gacgccgcac
    19381 tggcgatatt ctgagccaac tcggacgagc gtttcgcaat gtctgaaatt tcggcgaaca
    19441 cgtcaccggt ccggagagcc gaggcagagc cggcttccac ggcctgtgtt tcatgttcca
    19501 tcgcgaccac cgcatcctgc gtttccgtct gaatcacttt aacgaggtcg gcaatttcac
    19561 gcgttgcttg cgtggaactt tccgccagct ttctgacctg gtcggcaacg acggcgaatc
    19621 gcgcaccggc ctcaccggcg cccgctgcct cgatagcagc gttgagcgcg agcaggtttg
    19681 tttggttggc gatatcacga atggtcgaca cgatctggga aatttcgagc gaacggtcac
    19741 cgaggccctt cacctgcttc gacatgcgtt gcaccgctga acgaatactt tgcatgtcgc
    19801 gcaccgtttc ctgcaccgcg acgtttccgc gttccgtggc tgacaacacc tgcttcgcag
    19861 aatctgagga ggcgcctgct gtgctggcga cctgacgcat accggccgcg agctgttcga
    19921 ccgcgcccag cgtccgtact gattcttcgg cctgcgcgcg ggcggttccg gacatctgtc
    19981 cggcagaatc ccggagcgta ccggccgact tgttcacacg gtcagcggct tcacgcacct
    20041 gtttcaacag ttggccgaat cgcgcgatca tgaggttgaa accgtcggcc aggtttccga
    20101 acatgtcggc ggtcacttca cctcgacggg tcaagtctcc tttaccgacc tcggagacga
    20161 gtacgaggaa ttgcatcagg cgtttctgca tcaagtcgcg ttcttcctcc gtcgatacca
    20221 gagccgtaat acgatcgagc atggagttaa acgctgtggc catctgccct aactcatcat
    20281 gggattcaat tttcgcacgc gcttgcagat tgccgccggc ggcctgtgtc gcgacattgg
    20341 cgatgtggga aatgtttcgg gacaagaagg tagccaggag gtagccgacg aacaacccga
    20401 gggccacggc gacgagacca cctgcgacga gaatgaacgt accgttggag gccagggatt
    20461 gggcgtcgtc gttcagatcc ttcgccaccg attcgatgct gctgacctgc tnnnnnnnnn
    20521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngattgggcg tcgtcgttca gatccttcgc
    20641 caccgattcg atgctgctga cctgctcgtt gtggcgtcgt acggcgtttt cgaatgaggg
    20701 ggtcaagttc accgtcaagg ccaagatacc gagtgctcgc atttgctcag cctgtgccgg
    20761 agatagcgta ttgcgatcga aactgtcggc catggcactg agcgcaccat ccgcttcctg
    20821 aaaatatttt ttgagggccg gctcgaacag ggtgaggtcc ttcatttcgt cgcggcctga
    20881 tcgagacact cgcaaattgg tgctcttgta gttgttgatg gctttttcga tttcagcctt
    20941 caagggagga agcttggtga tttcctgcgc gaagtcactt tgtttcgtgg cagaggcgac
    21001 gtccagcaga atctggtgat gcttggtgag cgctgttccc atctgcgcga attccgccag
    21061 cggcaccgtg taatccacgt acacggtttg cgactgggca ctgagctggc gcagggtgaa
    21121 cacaccgacg ctggccatga tcatgatgat gaaactcacc agaccgaaac tgagcagcag
    21181 cttcgatcgt gtcttcatat cctggaatcg attggtaacg ccttgcccga tcatggtgcg
    21241 ctcctccttg ttactgcttt ctcaatgttc gctggccggt cgcacaatcc ctgctcgcgc
    21301 tgacccgcaa accgtcatag ctttattcac tacacgactg acaatgccgc actccccccg
    21361 tccacttgcg cgaagacctg cggcacctcc aggacgcctc ccagccggtg atccacgcgc
    21421 agcacggccg acacacaggg tctcgcacca gccggtcccg cctgcatggc aggcaacaga
    21481 tgctcgcggg gcaccgtatg gatttccgga acgtgatcga cgagtactcc cacctgcctc
    21541 gcgccgtgac gtatcaccac cgcgaagggt agcgccgtgt tgttcactga caagccaagg
    21601 ctcccgcgca gatcgaccag cgtgatgacc gttccccgga ggttcgtcac tcccgtcagg
    21661 taactgggca tgctgggcac ggccgtcacc gcttcgactt cgaacacttc gctgacatga
    21721 cgaagatcga tcgcgaagag ttctcctccc agcgtcaaca cgcacatgcg cagcgcgcct
    21781 tcggtcgatg cggcagatgt cctcgatgaa ccggaggatc cctgagccgg agaagtggca
    21841 gaagcctgag gctggatgat tggaacgtcg ctcatgagtt agttcaatgc ccgtttgacc
    21901 gcggcaacta aatcttcggc cttgaacggc ttcactacgt agccgttcgc cccctgttgc
    21961 tgcccccaaa acttgtcgct ttcctgtccc ttggaggtgc acagcacgat cggaataccc
    22021 ttgaaccggt catcactctt caagtcgcga caggcctgga atccgttgcg cccgggcatc
    22081 accacgtcca acacgatcag atccggctta tccaacgcca acttatcttc gagcttgtcg
    22141 gtattggggt aggacaccac ggtatgattg gcagacttga gatacccttc gatcaactgc
    22201 agttcggcgt acgaatcatc gaccacaacg attttgctca tgaatccatc cccccttcac
    22261 aaccattcaa aagttttcga cgcctgtctg gactgcgccg tcgtgcctac ttcataggaa
    22321 tgacaatgct gatggcgccg gccagatcgc cctcatgcca gccttctttc ttcgcgcccg
    22381 tgacatcacg ctcgcctttc ggattcccat ggcagctgag acatgtggtc gcggcatact
    22441 ccggatccat catgcgcagc accggtcttc catccagcat ggtggccttg ctgtacggct
    22501 gtcccttagg atggcgcgag tctgaaaaaa tgcgtaagac ttcggattca aaatcatccg
    22561 gacggttgcc tggaaaccga tagtcgatgc tcgtcagttt cacgcgaatg ccggtcttgc
    22621 gatagaaccg ctcgccggac ttgcgtgcga acacggcggg aatgatgcct ttgaagccca
    22681 cgccctgctt attgattacg ggctgcgcct catcgatgac ttctttctcc gattcgacca
    22741 tagccaggaa cagcggcgct tgcggcacgg tggccgggcg gctcaggtcg atcttcgtcg
    22801 ccttacgaaa ccggtcgatg acctggttcg cgacatagtc cccggtaaat cccttatcgc
    22861 ccttactggc atcattgatc gtcgcctggt tttccgacag aaccccgcgg ccgacttgca
    22921 gcaatttaat taataattca gccgtttcca cctcggtatt agccgacgcg gcaacgggcc
    22981 cgcccacaca ggccgccgcc aggacagcca tcgcaattct gtgaaaaaaa tgatttgcca
    23041 tgccccctcc tcctgccgat ctcgatgtcg ctcgtgagga atccgctcgt actaactgcg
    23101 ccaaacctct gtcaaagata gcgcaaaatt agcgattgac aatttttggc ctcgtgacag
    23161 gtacgtcagc ccctgcagcc ccggcctgcc tcgtgcacgt tcggtgccaa acaaaacaat
    23221 tacttagtat cttcgaccag gggaggagtg gaatgaaatg aagtcggcga ttttgcgtat
    23281 gattagatgt ggacgatcaa ctatacccac tatgatcagt gcgtctgagc agggttctca
    23341 attgttcaac atttcgagcg gagcgccaaa gtttggtcgc ttgacgggag aagtgtcaaa
    23401 atgacgtgga gctggaatgg ctagacactg tcagacgaaa tctgatcaga ctatcggagc
    23461 catgacaaaa ccggagcgag atcaaggtgc cgatccacat gatcagccca gcgatccatc
    23521 gcgttcagac gtgtgtcgtt cacgcgttga gactcttcca gagaaaccgc cggtaacccc
    23581 ttgcggctgc gcagccgatt cagccaagca cggcgaaaat ccggttgatc gaagagacca
    23641 tggacatagg ttccccatac caacccgtca tgccccaccg caccatccag acattcctca
    23701 tcgcgaacga ttgccgtgct gccgtccaga ctggtctttc ttcgcacgag ctgaaaggag
    23761 ggacgaagcc ccgcgcgatc cgttctgccc acgtggatga gatatcctcg gacagtcaaa
    23821 gtcgttgatc cctcaatgtg cagagcctgc ccctcgacct gctccgtaat tttgtctcgc
    23881 gccaacacgg tggtgaccgg caacaattct aaccccttat cgtgaccgcc ggtctcaact
    23941 ccatcgggat cggcaattga ttgtccaagc atttgatagc cgccgcacac gcccaccaat
    24001 tcccctccac gccgcacatg gtcctgcaac acactctgaa atcccctctc gcgcagatac
    24061 gcaaggtccg acagtgtggt cttcgaaccg ggcaggatca tgacgtctgc gcctagtgcc
    24121 gcttcaggcg ttgcgacata tcgcagcgcg acatccgttt cttgcgcgag cgcattaaaa
    24181 tcggtgaagt tactcatgtg cggcaggagc agcacggcaa cattgacacg ttccgccgtg
    24241 aaaggaattt gcgcccgggc acctacgtca agactgtcct cctggtccag tgcgagatcg
    24301 cgaaggtacg gcaacactcc gagtatgggt attcctgtgc gtgcggtgag aaaacggaca
    24361 ccctcgtcga agagtgtcct gtcccctcga aatttgttga tcacgatccc gcagacacgg
    24421 gcgcgctcct ccgctgcgat caggtccaac gtcccgacga tttgggcgaa gacgccgccg
    24481 cggtcgatat cacccaccaa caccacccgg gcatccgcca gttccactgt agtccagttg
    24541 acgagatcac ggtcgcgcaa attcacttcg gcggcactac cggctccttc gatcacgatc
    24601 acctcgtatt gcgctgcgag ccgttcgtaa ctctcgcgga ccgcgcgcaa caagcgggaa
    24661 tcctgcccgc gcgtgaaata atcgcgtgca tccaatgtcg caaagacttt cccctgcacc
    24721 accacttgtg atctggcgtt cgattccggc ttgagcagga tgggattcat gtcgacatgg
    24781 ggagcaaggc cgcaggcctc ggcttgcaag gcctgggcac gcccgatctc gccaccctcc
    24841 ggtgtggcga atgagttcag cgacatgttt tgcgctttaa agggggccac gcgaattccg
    24901 gcgcgatgca gcagccggca gagccctgcc gccaccatgc tcttcccgac atcggagccg
    24961 gtcccgagaa tcgcaatggc tttggcacga gaaggatgag gcatgagaaa gctcggaggt
    25021 taggtccgac gctgatcggc ccactgtgct gcgagggcca agccttggag aagacactca
    25081 gccacgacgc gaccgatcat cgacccgatc tctgtatggg tccccgcata cggataccag
    25141 gggccacgcc ctcgaagagc cgaggcgacc accaccgcat ccgttcccgt cccggtcgca
    25201 ccggcaagtc cggtccagct gggaacggca tgatcgcgca acacgcccgt tttgctctcc
    25261 gtcgccacct gcaccgcgcc caccatggca ggaaccgcaa gacagccgtt cgtcaccacg
    25321 atgaggttaa tcgttcccgc tgtcgcaccg atctgttcgc cttgcacctg acgaactggc
    25381 tccccggccc gcacggcatt cgtcaccccc acggtggcaa acccctctac ccacgttcca
    25441 tctcgctcct ctcgatgggt cacaagctgc gtcatgggaa ccgccgtcat gagtccgaca
    25501 cagtccggtt ccacgccgtg cgtctccgca agccgcctga ggtaatggga tggatgttcc
    25561 caggttcgcc ctgtagtgtc cgtcccgggc ggatgtgagg gcacctgatg attcaggata
    25621 aaacgagtcg tgcgaagccc tccgccccgg ggagccgagg agaggacgcg catgcgtacg
    25681 ccgagatcca cgatcaacgt atcgccggcg acccaatgcc tcgtcgagat gcggtttgca
    25741 tgacgctggg tcatacgcga ctattcccga tgattttgga taaagagttg aggaggcgat
    25801 cattgtcggc gcgcgagcga acggccaggc gtatcgaacg atcattcagt cccggcacct
    25861 gcgaacagtc tcgaaccaga attccttgcc gccgaagggc cgataccacg cgcgtggcct
    25921 tcagtcttgc cggcagctcc acgagtagga aattggccgc ggacgggaac gtcgaacagc
    25981 cgggtaatcg cctcaactcc tgcacaagcc gcgatcgctc ttgttcaatg aatcggaggc
    26041 ttcgccggct gtgccgcctg tcctgcagtg cgagttgagc cgctcgctga gccagcatat
    26101 tcaccgacca gggaggctga tgcgcgctca cttgttttat gaccttggga ttccccaccg
    26161 cgtagcccac acggagaccg ggcaatccgt aaaacttggt gaagctgcgt aacacgaggg
    26221 tccgcgcagg caggtcttcc ggcagaattg agacctgctc gcaatattcg gcaaaggttt
    26281 catccacgag acaccattgt ccaagacgag ccgcccggcg ggccaatttt ctgaccatga
    26341 cggcatcgca ggcctggccg gtcggactgt tggggttaca cagcatcacc gcatcaacag
    26401 gcggcatgct gtttcccttg ttccggaact tgtccagatt gtccagcgcc tgctcaagcg
    26461 gaggccggta tccgtcgtca cgtgtggcca tgatgctgct gatctgccca ccgacgcgag
    26521 ccatggctgc ggcatattcc gagaacgtcg gaccaataac gaggagatgc ctgatctgca
    26581 aggcctcggg aagcaggtga atcaattccg ttgacccgtt gccgatcaga aattcttccg
    26641 gtgaacgatc ccaaaatgtg gccaacgcct gccgtagatc ccgacaggcc ggatccgggt
    26701 aatggctgag cagttcactc ccccggccga tcgctcgcca cgccgcaggg gacggcccca
    26761 ggggattgat gctcgcgctg aaatccagca ggtgccggac attccgcttc aattcacgag
    26821 ccgccgcata gacatccccc ccatgagcct ggcggttcat gatacccata gggaaagcag
    26881 ggcgaagaac agacccagac catacgtcac gatcatgatg cgagacgcct ggcggatgtg
    26941 ctgcggagtg atgtcggcag ttccgtcacc gatggaggga gcttcgtgcg ccacaccgtc
    27001 ataataattt gttccaccca gttgcacggc caaggcgccg gccatggctg cttccggctg
    27061 gccactgttc ggactggcgt gtttatcccc gtctcgatgg agcatgtacc aactttcatg
    27121 aatccgatgc cattggcctg ttgataggcc tgcagccagt gcgatgaagc cgccggccaa
    27181 tcgtgccggc acccaattca gcacatcgtc gagtcgggcg gaggcccaac cgaaatgctc
    27241 ataccgggca tccaggtgac cgatcatgga gtcgagcgta ttgaccgcct tataggccag
    27301 cgccagcggc gccccgccca gggacagata gaccaggggt gcaatgatcc cgtcggatgt
    27361 actttccgcg atggtttcca ccgtggcccg ggccacgccg gactctgtca gcgtggcact
    27421 atcacggccg acgatcatcg ccacagcttg gcgggcaccg gccagattgc cggcagccaa
    27481 ttcacggagc accgcctgaa cgtggtcgaa caagtcccgc cccgccagcg tcgtatatgc
    27541 caatccaacg ccgaccacct gcccgagcag aggagcgatc gccgtggctt gcgcaatcac
    27601 ccaagttgcc gcggcataca ccgcgatcgg taagcccagc gccaggcagc cccccgcaat
    27661 ctggagggcc tgctcactcc ggcaaaggcg gcgaacacga tcatccaccc aggcgatgac
    27721 ggctcccatg cccttcaccg gatgaggcaa ccagcgagga tctcccgccg cgacatccag
    27781 cgccgcggcg actgctaact ccacaccagt catgcggcta ccaccaacgc cggaatcaat
    27841 aagagaaaga gcatctcgac cacttcgttc gtggctccga gcacatcgcc cgtcacgccg
    27901 ccaagcaagg tcagacaccg ctgcctgatc aggaccagca acaggacacc catgcccatg
    27961 gtcgcgaccg ccacagttcc gcccaaggcc atcgtcaacg ccacagccaa tacgaggctc
    28021 gaaagaagaa cctgttgcca ggacaggtgc gtgagaaacg aggcagccaa tcccccatca
    28081 cgccttgctg agggcgacgc ccatgccaaa ctaaccatcg cccagcgacc cagtgccggc
    28141 atgcacagca gcaccggcag tcgcagcggt gcaggcagcg ccagtaaccc tgcatatcgt
    28201 agcaacaggg aaagactcaa ggcggtcgcg ccgaacgcac cgactcgcgg atcgcgcatg
    28261 attcgcaaac gatcctcaac ggttcggccg ccggccagtc catcgaccgt gtccgccagg
    28321 ccatcctgat gcaacccgcg cgtgagcagc acgagcagca caatcaaaag cgcattgccg
    28381 acctccgctg ccatccacga gcgcaagcca acatcggcca acgccaccag gccgccgatc
    28441 gcgagaccga cggtggaata ccaggccatc gatgacgcca actcctgagc cgtcggcgcg
    28501 tgatgagttc ggctgatggg gatcgccgtc aagaaatgcc aggcgaacac aaacggtctc
    28561 gtcatggcgg caatttcagt cacgttccgt cacacccgct tcatcgaagg tcgccatctc
    28621 ggtgagaatt ttgattgagg cctggacgag ccccatcccc aggcaagccc ccgtcccctc
    28681 ccccagccgg agatccagat cgaggagcgg cttcagagcc agacattcca gagtcgcctg
    28741 atgcccccgt tcgaccgacc ggtgcgacgc aatcaggtag tcacgacaca acggctgcaa
    28801 cgcgacggct atcaaggcgg cggcaccggc aataaaccca tccaacacca ccggcacgcg
    28861 tgctgccgcc gcgcctagca tgagcccggc cagcccggcg atttccagtc cgcccacctt
    28921 ggccagcaca tcaagcgcat cagctttatc aggacgatgc cgattcagcg cctgctcgat
    28981 gaccgaaacc ttgcgggcgt gaccggcttc gtcgatgccg gttccacgat ccgtcacttc
    29041 agcaaccggt cgaccggtca tgacggccgt gatgcccgcg cttggagtcg tgttgccgat
    29101 ccccatttcc ccggtcccga taaggccgac cccctcctgc gcagcctccg tcgccagatc
    29161 gattcccacc tgcaatgcct ccgtcgcctg cgccctggtc atggccgctt cacgcaggag
    29221 attgttcgtc ccccgcatga tctttttctg aatgagtccg ggcacgaccc cgaagtcgta
    29281 ggcgacaccg atatccacga cccgaacttc cactcccgta tgtcgcgcca ggacattcac
    29341 cccggcaccg ccccgcagga aattcagaac catctggggt gtgacctcac tgggataggc
    29401 gctcacacct tccatcgcca cgccgtgatc agctgcaaag gtaaacacga cgccacgcgg
    29461 cacattcggc ttcacatcgc ccgtgatcct ggcgtagtgc acggccaact cctccagccg
    29521 ccccagactg cccaacggct tggtgagtcg atcaaggcgt cgctgagcgt tcgccgcgct
    29581 gtcaccattc actggttgaa tcaggcccat cgcgtcatcg agtgtcatca tctctcctat
    29641 ttatttgagc ctgagcgact gaccgctgat aacaaagtag acctcgtcag ctgaggcggc
    29701 gatctgctga ttgacccgtc cggcgacatc acgaaaggcg cgcgcgctgc gacttgtcgg
    29761 caccaagccg aatcccagtt cgttgctgac cagcacaaca cgcgcgggac tctgtcgaat
    29821 ggcgtgcaca agttcatcga ctcgagcact catgtctgcg ccggcaagac ggcggccgca
    29881 gacattgctg agccagagcg tcaggcaatc gacgacgatt gtgcgatagc ccgatccgtc
    29941 cgatcgaaac cagcccgcca gatctcgtgg cacctcagcc gtcacccact cggcggaacg
    30001 tgtcgcccgg tgccgcgcaa tacgcgcctg catttcctta tccaatccct gcccggtcgc
    30061 gacaaaggcc tttggcgctt ggctgcctgc ccgttccaat gccgcctggc ttttccctga
    30121 ggaggcgccc cccagcacca gaatgaggcg ggaccctgca cgcccgggcc tggtcgtcga
    30181 tttacttgat cggggcatca gattcacgct gccacaaaaa ctccggctca ccttgggttt
    30241 tggctaccca tcgtgccatg acgaacaagg catcgctcaa ccggttcagg tacttattga
    30301 gctcggcgga cacgtcttcc tcacggctga gccggatgca atcgcgctcg gcccgccggc
    30361 agatcgtgcg cgcttgatgc agcgtcgccg acactttccc cccgccaggc agaataaatt
    30421 ccttgagcgg tgccaggtct tcctgacagc gatcaatcat ctgctcgagc ctggtcacgt
    30481 cagcctcggt gactttcggc atattcggaa agctctggcc cggcgcggtg gcgagcagac
    30541 tcccgacatc gaacagttta ttttggatcc agcgcaagtc cgtctcgagt tgcgcggagg
    30601 cgggagaggc gctcccgcct tcggcattca tcgcccgcac caatcccaca gaggcattca
    30661 gctcatcgac ggtgccatag gcttcaacgc gcacacagtc tttccagacc tgctgccctc
    30721 cggcaagcct tgtttggccc gcgtcgccgg ttcgtgtata gactttggta attcgcatgc
    30781 tctgatcctt tcactccgga ttcgccactc gactcacgag gccgaacccc ggtatcgaca
    30841 cgttacctcg cttgatctgc ctgaggcggc actcccggtc caagctttcc atcggccacc
    30901 tgttgcaggc acgcgggaca gagacaatcc tgataacggg cggcgatcca atccatttgg
    30961 gcttcggtaa tgccgatctt cccgcaccaa cattggtacc cgacacactc gaacggctga
    31021 cggcaatgct cgcacgtctt gctcaccggg cctctcaaaa ctccacccct gcctgtgcct
    31081 tgatcccttt acgaaaggga tgtttgacct gtttcatctc ggtcaccaaa tcagccgcgt
    31141 cgatcagctc agccggagcc cctcgaccgg tgatgaccac atgttgcatg agtggacgag
    31201 cctgcaatcg cggcagcact tccgccacct ccacatagtg gtggtgcagg gcaatgttga
    31261 actcgtcgag aatcaccatc gcataggagg gatcggtcat gaactcgcag gctttgttcc
    31321 aggcctcctg agcgaatctc gtatcgcgtt cgcgatcttg cgtttcccac gtgtaccctt
    31381 cgcccatgcg cagaaacatc acgcgatcgc cgaatgacct aagaatacgt tcctccgccg
    31441 tatcgatagc ccccttgatg aattgcacaa tcgccacctt catgccatga ccgaggcagc
    31501 gaatcgccat accgagcgcg gcagttgttt tgcccttgcc cgcgccggtg tagaccatca
    31561 gcagtccctt ttcctcctga gctgcttcga tacgacgatc gaccgaccct ttcaggcgct
    31621 gcattttggc ggtgtgttca tcccgatcag tcatctcact gcttcctcat gcttggcgcg
    31681 cagctttctc ttgtcgcaca gactggagca gattgaccac cacctcggga cggctccgga
    31741 aatgctggtg actatacaat gccaaggtgt ttctgaccat cacgccatcc tgaccaaccg
    31801 gcttgcccgc tgcatctgcc agcacacagg catacgccaa ctcgcccttc ggctcgagga
    31861 gggaataatg gaactcatgc ccgcgcactg acgtgccggc cgggcccagc agagagggcc
    31921 gcatcgtttc gacgatccgg taccccaacg tcatccccga cctccgcatg accgcctcgg
    31981 caggaaagac accgaccatc tcatagcgat gaccgtctcc atcacgaatg gcttgcgtca
    32041 aatacatcaa gccgccgcat tccgcataga tcactccacc gcggtcgctg aacgccttca
    32101 ccgcggcccg catgctcacg ttggcagcca atgcctcacc atagagctca ggatagccgc
    32161 cgccgaaata caacagatcc acatccggca gagctgcatc atgcaagggc gagaattgta
    32221 cgatctcagc accggccgct gcgagtaatt ccaggttgtc ctgatagtaa aagcaaaatc
    32281 caccgtcata ggccacacca acacggaccg tttctttcgg tcctgccgca ggtggactga
    32341 ccggatcttc ttcaaaccat ccttcagccg atcgggcgat cgcctccact cgagccagat
    32401 caatcgtctc ggcagccgac tgcgccagtc ggtcgtagag gtcacatgac cctgcctcaa
    32461 tggcggtcct gagaccgaga tgccggtccg gaatcgtaac cgaggcatca ggccgaaggt
    32521 acccgacgac ggcgagcccc gtctccgctt ccaccgcctc ctggagcaag cggtagtgcc
    32581 cgtcgcttcg cactcgattg aacaacacgc cctgcacaga aaggcccgga tcgaatcgcg
    32641 catatccggc tgccatcgcc gccgccgagc gcgccatggc gctgccatca atcaccaaca
    32701 acaccggcgc atgcaactgt ttggccaatt ctgccgtgct gccctgctcg tgtatcgggg
    32761 aactcccgtc aaagaggccc atcatccctt cgatgatgga cagatccgca tcggccgacg
    32821 cctgcgaaaa gatcgaccga ttgaccgcct cattcaacat ccacccgtcc agattgcgcg
    32881 agggccgacc ggtcaccagc tgatgatgtc cgggatcgat aaaatccggc ccggccttga
    32941 acggttgcac caccagccct ctggccttca acgcggccat cagcgcaagg gtgacggtgg
    33001 ttttgccgac accgctgtgg gtgccggcaa tgaccagacg agggcgtggc atcaccagac
    33061 gatgctcatg gaccatttac gatttaccgc cgaacgtcac gcgaagacct ccgaaaatcg
    33121 accggatcgg cgtcccggca ctggcgattt cttcatactt ctcattgaac aggttctccg
    33181 ctcgcagata ggcttgcatc tgcttcgtga catcgtaggt cacggcgagg gaccacacgt
    33241 cgaacgccgg catggcttgc cgatcaccgg tcgtattgaa tcgcgacccg acatagcgcc
    33301 cgatcaccgt gatccacaag gggtcgatcg gttgatagct cacggacgcg ctccactggt
    33361 cggtcggcca gcgtggcaaa cgcgtttgcg tatccagatc gcgcgtgatc gtattcgtgt
    33421 actgcgcctg aaccaccacg cctttcagaa acggccgatc gctggaatag gtataggtga
    33481 gcccggcctc ccaccccttg gtcgatgcct cccccagttg ctgcggacag aatccaaacg
    33541 tgctgaaggg ggcgcaaaac cccgggtcaa aggtcgtgac gatgagattg cgataatgat
    33601 tccagaagaa gcctccgctc agcttcaact gtttcgaaaa aaaatactga tcgatccccg
    33661 cgtccatact ttggttcttc tccggtccca gattgggatt cccgaagttc ggaaagtaca
    33721 gctcattcat gctgggtgcc cggaagccgg tcgaatagcc gacgcgcaat ttcgtatcgg
    33781 tttccttctg gtagtacccg ccggtgagtc gatacgtggt ggcattgccg aagacgttgt
    33841 aactgtcgtg ccgcacaccg gccgtcgcga agacacgatc ccagagattg aactgcgcct
    33901 gcgcaaatcc ggcgtgggaa ctcaaaacgc gattgatcag gcccgtgtcg ttttcaccct
    33961 gctgttcccg gaattgatac cctgcgctga gcaggagcag cctggtaacc tggaaattat
    34021 gttgccactc caggcgattg gaaaggacac gcgtttcatt cggagatccg aacggcgtgc
    34081 tgatgaatcc gtcgacaagg ctccgctgta cagtccccgg cagaaacagc gaagcttcct
    34141 gcgatcgtgc gagtgtcagc ttctgggacc accagccggt gatcggctgc tcataactcc
    34201 cgctgaacac atactgctga ctgcgcatct tcgacccgta gacatccgcc ggaaatgtcg
    34261 ccgagacatt gtcgagttgg gtatcggaat tcatccagcg catggtgaaa tcgagtcgac
    34321 catcacgcgg gagatccacg ccgatgcgcc ccgatccctg ccaattacga aaggaatccc
    34381 gctcggtcgc gccgcggcga taattgacgg ccgaaaagcc ggaggtgtcc caacgggaca
    34441 gcgaaaggct gtagtcgacg agtccctgct ttcctgatac ggtgccgccc tcgcgcaacg
    34501 tcgcgaagga cccatattcc atgaaaccgg tcgcggtaag cggcccctcg cctcgcttcg
    34561 tgacgatatt gatcacgccg cccatcgcat ccgaccccca caacatgctt tgcgcgccgc
    34621 gcaggatttc gactcgctcg atgttgtcgg tggtcaggtt ggaaaaatca tagctgccca
    34681 acgttccgct gttgacgatg gcgccgtcga tcaggaccag cgtctggctg gaacctcccc
    34741 cgcgaattct tgcgttgacc tccgttcccg gtccgccgct cgaaaacacg gccagccctt
    34801 gcgccaaccg tagggcgtcg gccacgtgcc ggatattttg ctttttcatg tcttgcgccg
    34861 tgatgacttc gacggcgctg gtcacctgcg tcacagggac cggtgttttc gtggctgaga
    34921 tgacgacttc ttcggcctgc acgaccggtt caggctcctc cggttcttcg gcaccggcga
    34981 ccgccggcca gacaggcggg gtgctcacga tcaacagcaa cagacaacag acagaccatt
    35041 ccctgacacg cttcaacatg ctgacgttcc ctttcgctcg aagggctcag tcgtaacacc
    35101 cgtcagttgg gtctcctgac ttgcggatcc tggcttccct gcgccttccc gacggcgtga
    35161 gacgtgagtc atcgtgaaac ggtcagagga aacctcttgc gcttcacaag ggatgattca
    35221 cgaatcacgt cgctcgccag tggcttcctg cagggtcact ccccgcttac agtggcggca
    35281 ccgtgatggt tttgcaccat cttccccgac gctgacggcg tcttcctgat actccgttct
    35341 ccaatgatga catgcccctt ctcatcacgc gggtgggccg ggcacgaacc cctccgcaac
    35401 gttccgttcc tatcggtgaa ccggcacggc aggcccgggc agtgtgatac gcggcaatcc
    35461 cgaatcggga tgcgcatcga tcagcacggt gcaaccgtag acctcacgca aggtttcgac
    35521 tgacatgacc tctgagggcg tcccgatcgc ggccactctg ccgtctttca gcatcgcgat
    35581 gcgatcgcag tactgactgg ccagatttaa atcatgcgac acgatgacca ccgtcaaccc
    35641 ccgctcctca cgcaatcgac gcacaaccga gcaaatgtcg agctgatgct gaagatcgag
    35701 aaacgccgtc ggctcatcca gtagcaatac gcgaggcatt tgggcgaggg cccgtgcgat
    35761 catcgttcgc tggcgctcac cgccggagag atcggtgacc agccgtgagg cgagatgcgt
    35821 aatatccatc gtctccatgg cctgcgcggc tgcccgacaa tcatcctggt cctcccaccc
    35881 gaaaccaagg ctccagcgcg accgatggcg atgcgggaat cgccccatca acacggtttc
    35941 cgccaccgta aatggaaata gctgcgggtt ctcctgtggc acacacgcga cggctcgcgc
    36001 cgtgtcgtcc tgcgacaggc cagccaggct ctg
//
LOCUS       DAOQDS010000042        34052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_431_length_34052_cov_20.032532, whole genome shotgun sequence.
ACCESSION   DAOQDS010000042 DAOQDS010000000
VERSION     DAOQDS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 34052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34052
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_431_length_34052_cov_20.032532"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..1155)
                     /locus_tag="PK589_11640"
     CDS             complement(220..1155)
                     /locus_tag="PK589_11640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNJ20301.1"
                     /translation="MKRSQVFLVIAVTFCVVICTWQSSYAAGFSLDQLRDNFIANIND
                     GNFILPKTSTGGLVTADFTSELAAGSRVGLAGAKAGFIAITGVNGGILGGIAGSFVPG
                     LGTAVGAGIGATIGLAAADRFVTTLNSGRVGDPFVADALLVKSISVGDKNVTQEVMAL
                     GQANVQRTIGLAGPSPGSFSFIAPSIFSPKLLDASGAVVSKVLGVDTFILNSPLGRSV
                     GVVAYNDPQITGRTVQRTDLFALNLLQITDQPVGTRFALTATPFVVAATVKEQLIDAA
                     PEPGTLLLVASGMVGFGGWRRRVAGRFTLSASDQV"
     gene            complement(1482..1847)
                     /locus_tag="PK589_11645"
     CDS             complement(1482..1847)
                     /locus_tag="PK589_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ20302.1"
                     /translation="MTSCSEPLSEQAAKHEMRGDGYVQQEQFREAVIEFKNVVRAAPD
                     NPSLQWKLAKAALKGGDPSTAYQALGRVVQLDPSHFEAKWSLGDLYLAGGKMDEVGKI
                     AEELVSAQRPIALVTPPTT"
     gene            complement(1907..2707)
                     /locus_tag="PK589_11650"
     CDS             complement(1907..2707)
                     /locus_tag="PK589_11650"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNJ20303.1"
                     /translation="MNRCNWKKLSLSLMCVAILGLTAPLAQTTAHAVPLYGFTGHDVP
                     FSASPVISATVNYAVLPPFDPFGSMLSQLYVPAAGNPGFDSSKYTYLYQIANPASSHA
                     IWGSFQPQMSVVTPVSATSTGSFASGDLRLDFLNGGSLVNASGNNLQSTHSFGIGART
                     VSGAERIEILSNPLGGRSHPYSTFLNQGLTPGFTSQLFGFQSSNGPTFTTGLLQGDIF
                     APGSSFAAPVSLFATVPTAGAPEPGTMLLIGTGALSLLAWRWKKPSEP"
     gene            complement(2970..4955)
                     /locus_tag="PK589_11655"
     CDS             complement(2970..4955)
                     /locus_tag="PK589_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20304.1"
                     /translation="MIPPSAAIRVCIDCLLMMALLMIAPGELHAIQIEPNPNPDANTI
                     TITPSTPRENLVPFTNVGTINIEPLASFHNANQFNNSGVINNRGTITNSGTFTEVVRP
                     FPNPGGGFLGLGEGSQFINLASGVYNGGDRTTGIGGTFVNEGAVTHGLGFIRVGETGQ
                     YIQRQAVGSFVTPTTITPDAPFTNAGRIHIAAGDFNIDPISIRAAVSRYEQASTGTTQ
                     IDARFQNIGGEVHNAGTITVGSTGEYRQEGFPGPILAGSTTNTGTFTNAGTTNIGTGV
                     FTNQGSVANSGMFTVEAGGTYSQSRGDGTGAPVASTSNSGTFANAGQVRIGEGTSFGN
                     FSNRTPPVGQYIQQASGTTQIYGTFQVGGARVENAGLMTVGTTGTYLQAFNKFVPVVS
                     TTVNSGIFTNAGSTRVDAGTFTNHGSVVNSGTFQIAVNGVATLINHGTVVNSGTFEVG
                     AGVGAVGGVSGTGSYSQTTSVAHTIVNGTFGNTISLQAGSLSGTGTITGAVVNTGGVV
                     QPGTSLVPGTLTLANYTQGIYGRLDLKIGGLLAGSQYDVLKVTGSGTFGGSLSVRLIN
                     GFTPGLGNTFSLLTCSLGCTGLNSGTLFGGGVSLPSLASGLAWLSGLTNGGNSFSLTV
                     VASAASAPEPSTLLLIASGFVYLVIWQWRQLRSVHRS"
     gene            complement(5112..5780)
                     /locus_tag="PK589_11660"
     CDS             complement(5112..5780)
                     /locus_tag="PK589_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ20305.1"
                     /translation="MFEGDFQQRRTRVLIADEMALIAAGTRALLEPDCDVVGTVADGR
                     ALLSEAETLRPDIIVMEILLPLLNGLDALRPLVRKVPESKIVLLTAQESSWHVAEAFK
                     AGASGYVLKRSQPSELTQAIHAVLMGQWYLTPLIAKNVVKQDVGGEQPKRKVPTVSTL
                     TPRQREVLQLIAEGRGTKEVATLLNIAVKTVEFHKFRIMDQLNLHSTVALAKHAIIEG
                     LVRL"
     gene            complement(5919..6308)
                     /locus_tag="PK589_11665"
     CDS             complement(5919..6308)
                     /locus_tag="PK589_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20306.1"
                     /translation="MTTPKGPSKTGKIKKSTTSSSPLARKALAAKWRQKKDDVSAAME
                     GSQRQGAMETSPKISAALVRTVEAAKLRRKKEMITGAMEKSQRGTDNHGKSQSEALAR
                     TVAANERLRTKTVITAALKESQRRSGS"
     gene            6532..6969
                     /locus_tag="PK589_11670"
     CDS             6532..6969
                     /locus_tag="PK589_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ20307.1"
                     /translation="MLTRLCCAGLVLALCLSTTPSRTLAESGGTGAPLKGDAARGQVL
                     FNGKGICHYCHGVDGVIDKKPSLKPETVAAIAKHAASAPDLRNRAVHTLKDNKARFRA
                     IREGHPGSGMFPDSTLSDQEITDLLAYLAALRQSASAPAKSPY"
     gene            complement(7079..7420)
                     /locus_tag="PK589_11675"
     CDS             complement(7079..7420)
                     /locus_tag="PK589_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20308.1"
                     /translation="MATVLLSGVAPVMAEGDQDWLDTMVQAVLAEQVKDGSAEEFLSP
                     YVAQLAAVRSHLLNGESEAVYRTMNRFMEMLQAREAGISAEAADRLFDYCYLVTPPRY
                     HDVSRHLHRLS"
     gene            complement(8152..8370)
                     /locus_tag="PK589_11680"
     CDS             complement(8152..8370)
                     /locus_tag="PK589_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20309.1"
                     /translation="MTTHETDIVRDGCLRCGGFMVPEEIREVNQMGWRCVMCGEHIDS
                     LILEHRRKMATPEGARQLLEKRDKARLN"
     gene            8542..9222
                     /locus_tag="PK589_11685"
     CDS             8542..9222
                     /locus_tag="PK589_11685"
                     /inference="COORDINATES: protein motif:HMM:NF021631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNJ20310.1"
                     /translation="MTHPPSPPTDTCILKVVVGSHAHGLARPDSDKDFRSVFVLPTAE
                     LLRIGFKYQGTRMMKEEDDETAWEIGHFLQLALQGHPLVLETLVAPVVAMDDWGAELR
                     QLFPRLWSAQTAYDAFLNYCDNQRKKMLEKKDSRPEKYAAAYVRVLFNLCELLERGTF
                     SIRISETPVGEAVVKIKAGQFRPGEVIDMGEHWRAEATRRLHTCSQKAHPEIADAFLL
                     RVRKAFLT"
     gene            9808..10281
                     /locus_tag="PK589_11690"
     CDS             9808..10281
                     /locus_tag="PK589_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNJ20311.1"
                     /translation="MSGLTRWEPFRTQMNPWRELEEVQKRLSSMWGGAPQKEGSQKES
                     ISVAEWSPLVDITEDEKEYLIKAELPEMKKEHVKLTVHDNVLAISGERKYEKEEKDTK
                     YHRIERAYGSFMRSFTLPEDADGSKVSAEYKDGILNVHLPKSAQVKPKSIEVKVS"
     gene            10297..10443
                     /locus_tag="PK589_11695"
     CDS             10297..10443
                     /locus_tag="PK589_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20312.1"
                     /translation="MFKHPRYLDIPWEMHWHPEETSHHWWLILLVIIAALFLIGVGVT
                     SGLW"
     gene            10498..11151
                     /locus_tag="PK589_11700"
     CDS             10498..11151
                     /locus_tag="PK589_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HNJ20313.1"
                     /translation="MFKNREEAGELLAQELVAFRNAPDGILLALPRGGVTVAYQLSLA
                     LHLPLDVLITRKIGAPGNPEYALGAVSETGAVYWNQEALSGLSLSERDLAVAVHAQQI
                     EVSRRVALYRQGRPFPNLKDRTAIIVDDGLATGATFFASVATVRQARPRRIIGAIPVG
                     PRSTIQEARSLVDQLVVLRVPEPFYAVGNFYRDFEQVEDREVLQYLNLAEEAFLSKS"
     gene            11202..11699
                     /locus_tag="PK589_11705"
     CDS             11202..11699
                     /locus_tag="PK589_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HNJ20314.1"
                     /translation="MMTNQPEGGHSARRDRFVEEYKHDSYKMPGKLKEPAVCKTCGAL
                     YHKGRWTWEIKPADAEQITCPACLRIQDKNPKGFVTLKGPYKAQHRDQVMGLIRNTEA
                     QEKKAYPLARIMTIEDREEGLVILTTDAHLPRRIGEALKHSHHGHLDIQYDQDEDFIR
                     ITWTG"
     gene            11765..12316
                     /locus_tag="PK589_11710"
     CDS             11765..12316
                     /locus_tag="PK589_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ20315.1"
                     /translation="MGEQRRGWSLASGLLTGGILVLVAMLPIAKAEVQLLPGAAAELD
                     QATANAVLKTFQQAEEALRGRHVDGVMALYSEQYDYHGLHKKDMRKVWVDLLDEFQDL
                     ADVHHFSRMSKIGSGSKTIIEVTCTGSLTGLSKTGGLRVPIDSWYEEVHYMTWEDGQW
                     RIRGNVGDSPRFMPFGTSPHPLF"
     gene            12519..13376
                     /locus_tag="PK589_11715"
     CDS             12519..13376
                     /locus_tag="PK589_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ20316.1"
                     /translation="MRVLIALDWSEQAFAAVREVSYLYDLQEVVLVHGIDLGMFQYPI
                     VAEVSNMQGYDEFRKAMEAAGQQLLDHTSTLLPSQGVSTTRVCEFAKPASLILDKARD
                     MRAELIVIGGRGRSRVGEFVLGSVSHRVALHADCTTLIVKEREGPVKRVTVAIEGHED
                     AERIKAWLLAHPFKNPVDLTIVSVVRPIPATDPFNLFPLQDWTGIAVRSAEDLVKNLA
                     AAVMNHRYSVGTQVAVGDPTDILTERARSSDLLIIGSHGRKRLERFLLGSISHALLHQ
                     VPCPVLLVR"
     gene            13426..13788
                     /locus_tag="PK589_11720"
     CDS             13426..13788
                     /locus_tag="PK589_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ20317.1"
                     /translation="MKTFSILCALSLVILGSSWAAGQTNRGNPRDGQAIYEKNCLRCH
                     GEKLDGNGPEGQYLIVRPANFQSVNSRAKTDWELLITIANGALFSPMHGYRGKLTDQQ
                     MLDVLSYIRSVATPDFVS"
     gene            13945..16680
                     /locus_tag="PK589_11725"
     CDS             13945..16680
                     /locus_tag="PK589_11725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ20318.1"
                     /translation="MQRIRPRQMSPLRDEGPGSAHTILSDGTTALLRLAQPVDAEELQ
                     RFFSHLTPAATRHRFFSESAPPAEVIRALCDPSHPERSLTVIVLRRHDDTLRIIASGS
                     YHARGPHEAEVAMAVDDALHGHGLGTILLERLALLAIRQGFTKLWAITHADNLAMREV
                     FATSGFTMEEHLEGGDMEVELSLTPTDQSVRQSEWRERVATIASLHPLFHPRAVAVIG
                     ASRAPRSIGYRLLDALHHNRFEGRCYAINPHASTIAGVEAFPSVRHLPTPVDLAVIAV
                     PKEAVLSVVDECAAIGVRALVVITAGFAEVGAEGRRLQDQLLEKVRLHGMRMVGPNCF
                     GILNTDPAVRLNATFTSTFPCAGSIAMSSQSGALGLALVAASERLHLGLSTFVSVGNK
                     ADVSVNDLLQYWEQDTATNVILLYVESFGNPRRFAQIARRVSRNKPIVVLKAGRTSSG
                     KRAAGSHTAALAANDVAVEALFQQTGILRADTLEEMFALATGLSEQPLPKGRRIGILT
                     NAGGPAILCADACEAAGLEVPELSQATISRLSPFLPASASLRNPVDLIASATPEQYEQ
                     AIVALLTSEDIDALIILYIAVTATDVDPIAQGIARGIEAARAQQLVKKPVSIGWMVET
                     DRDRRFSLPHETIPTYSLPELPARVLGKVAGYVQWRDRPLGMVPDFDDLDISSIKTVC
                     RSVLATRGNGWLTVTETRAVLSSMGISLPPGGLATTAEEAATIAAQIGFPVAVKLASH
                     TLVHKTEVGGVHLNLSSKAEVRRAYDEIAARLAQDHTLDAMEGVLVQSMVTNGVEVMA
                     GMVQDPSFGPLIGFGLGGIHVEILGDVRFRITPLTELDAADLIRSIKGYRLLQGYRGH
                     APGDVDAIQELLLRLSRLVEEVPDIVELDLNPIFALPPGQGYRIVDARIRVAGTG"
     gene            16701..17042
                     /locus_tag="PK589_11730"
     CDS             16701..17042
                     /locus_tag="PK589_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20319.1"
                     /translation="MLRLMQAGGSIQSLSAMTEHTLGDATGLMGPAPFVIQPPDYRLL
                     PQRRSGDDSATELMTRGAGVRPCHAKDEFPMGIAACLFRNLSQHPAMLCMATGTGLSL
                     NNERPVPSRHG"
     gene            complement(17289..17876)
                     /locus_tag="PK589_11735"
     CDS             complement(17289..17876)
                     /locus_tag="PK589_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20320.1"
                     /translation="MTGVRSRLLAVMTVSVAAMLSALAFEALLSLDKVTPFGHTGYGH
                     GVGWAGLAITLLVFVYPIRKRVNPSHRWPRGWFRVHMVTGVLGPLLIFIHSGAHYHAI
                     VPILAMGSMVIVVVSGVVGQLVHAFSLRALNEHRHDLLHRGLSESDVDAQLHGMASQE
                     EAFRLWQAIHAPMTLMFLAFTALHVVGALFFAGMS"
     gene            complement(17907..18290)
                     /locus_tag="PK589_11740"
     CDS             complement(17907..18290)
                     /locus_tag="PK589_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20321.1"
                     /translation="MNRRYAVTLRSLSAVLVLAGLLQGEHAWPDVGIPSSEGLWAPVL
                     RVADNAGTMPNPHSDLIFRATGATRCLDCHRAGKEGAISPQVQDNQLVQDLKAKAKGI
                     HGPGRFADCLRCHAGGNKGVEKYRR"
     gene            complement(18287..18769)
                     /locus_tag="PK589_11745"
     CDS             complement(18287..18769)
                     /locus_tag="PK589_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNJ20322.1"
                     /translation="MSHEKISVTFEQDHDRLDALFTTFQQQKRKDMAKAKEAFVEFKF
                     GLQRHIVWEEDVLFPKWEENSGMAEGGPTQVMRTEHRIIGECLEAIHQKVQASNPDSN
                     LEEQRLVDVLKSHNMKEERILYPSIDQVITDQERAELYQAMKDIPEERYRTCCGSGLA
                     "
     gene            complement(18784..19275)
                     /locus_tag="PK589_11750"
     CDS             complement(18784..19275)
                     /locus_tag="PK589_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20323.1"
                     /translation="MSRSKICLGGGLSFLFAMELFTTGCVKQQPAPSADEVRPVHLPE
                     VRTPIPLGPEARQEHRAVMLQHLETIQAIVAALADEDYRLAQGLTESHLGFFMHRYAM
                     ARQQPGDFPPAYHDLAMAHHAAAGKLADVMPTKDLKQILPEFNNVLKACVACHLEYRL
                     RHS"
     gene            complement(19377..19685)
                     /locus_tag="PK589_11755"
     CDS             complement(19377..19685)
                     /locus_tag="PK589_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNJ20324.1"
                     /translation="MADHDHNCSGLGVSIAFVSGAMLGAMAAILYAPHSGEETRRAIK
                     GYARRTEEDVLEKAKEIRTDIAHTMEEAKRYLKETEATIAAALAAGKEAFKKERADRA
                     "
     gene            complement(19951..20385)
                     /gene="trxC"
                     /locus_tag="PK589_11760"
     CDS             complement(19951..20385)
                     /gene="trxC"
                     /locus_tag="PK589_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HNJ20325.1"
                     /translation="MTESIHLVCPHCRSINRVPAARLAQQPRCGQCHGSLFSGHPIAL
                     TAEDFDLHAQRAEIPLLVDFWASWCGPCRMMAPAYEQAAQMLEPHIRLAKVNTEEEQT
                     LAARFGISSIPTLVLLRGGRELARQPGALGLQDIVRWVRSKL"
     gene            complement(20419..21009)
                     /locus_tag="PK589_11765"
     CDS             complement(20419..21009)
                     /locus_tag="PK589_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ20326.1"
                     /translation="MKRYLIILVSLLATMPVVSSESVMSSDPMAMGHDVMQPRVPADR
                     IAEARQLRNPLPDSAETIERGKALYTGKGTCINCHGSEGDGNGPVAAQLSPAPRNFQH
                     HGFWRHRTDGEIFWVIKHGSPGTSMIGFADQLTDEDIWAVIQYERTFAKEHGQGREMG
                     PPEGMGHRGMRGEMGGMGHRGMGSGSGRCEGERCDR"
     gene            complement(21016..22059)
                     /gene="ppk2"
                     /locus_tag="PK589_11770"
     CDS             complement(21016..22059)
                     /gene="ppk2"
                     /locus_tag="PK589_11770"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNJ20327.1"
                     /translation="MAADGQSKGKKDRAGTEPAEVFAQKDLSQDELEIIPTAVPRQGD
                     GYQVADTNGDGMADGLIGEAGKALTEDDLRRINTPRGLIQLIKSKNVDLEEVRKTLLY
                     EQELRQLQVELVRLQRWVQSDGQRIAILVEGRDAAGKGGTIRRFTEHLNPRAMRVVAL
                     PKPTDDERGQWYFQRYIRQLPNKGEIVFFDRSWYNRAVVEPVMGFCSKKEHQRFLQQV
                     TEFEHMLYEDGVTIIKFWFSISKEEQAKRFDARRQNPLKQWKLSPVDEKAQEMWDSYT
                     RFKEEMFSKTHTTFSPWIIVKANDKQAARLESLRYVLNLLPYRGKDEAQIRLTPDPNV
                     ITRFHRKMAELDF"
     gene            complement(22084..22443)
                     /locus_tag="PK589_11775"
     CDS             complement(22084..22443)
                     /locus_tag="PK589_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNJ20328.1"
                     /translation="MATFMISGSRGTDDPTMATLPFMAAKTAKEQGHDVVLWLWNEAV
                     TLGRKGTADHVTGVNLTPLKDLLAAVQAAQIPIWVCGACAVARQIGAADLVGGAAIKG
                     MPDYIKAVAERDRNVTF"
     gene            complement(22562..>23059)
                     /locus_tag="PK589_11780"
     CDS             complement(22562..>23059)
                     /locus_tag="PK589_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ20329.1"
                     /translation="SPNMPYWGLTIGQQGVADVMAYLKATFKGGADVAQAAISSDGPS
                     GVCPQPRRTAKAPADFLSKTSPLPHSDATIQAGKTLFLQTAQPVACAMCHGEKGNGQG
                     FMGAALIPPPRNFTCGAMMKDLPDGQLFWIIKNGSPGTGMMSFAGLPDEQVWQLIAYV
                     RSLAK"
     assembly_gap    23060..23159
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23160..23359)
                     /locus_tag="PK589_11785"
     CDS             complement(<23160..23359)
                     /locus_tag="PK589_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20330.1"
                     /translation="MGGRRTAMIGCALVILVGLFSFPRLLLGSDQTRVKEMIQNNCAG
                     CHRLEGKAESRFNLKAPDLIWAG"
     gene            complement(23396..24031)
                     /locus_tag="PK589_11790"
     CDS             complement(23396..24031)
                     /locus_tag="PK589_11790"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ20331.1"
                     /translation="MEGAQRERGRWSLLAAGLVLLLGIAAGTAAQEDDKHTIEPAQAR
                     RAVTLIHARCAVCHTTDLIVQQRLPPDRWQITVEKMMHWGADLSKDEAAALLQFVTAR
                     YHPGAPDHLPSIEEELALTAPAGGPSTASDGPLVGVPARGAEIFARNCQACHGEGAMG
                     GAGPKLARNKILKNEGVFWETVLHGRGPMPAWGSVLSHQEVADIHAWLASR"
     gene            complement(24040..25221)
                     /locus_tag="PK589_11795"
     CDS             complement(24040..25221)
                     /locus_tag="PK589_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HNJ20332.1"
                     /translation="MAVTRRALFERVLQGLGAAVVAGGVDRAFADSQSDVIGQVSGPL
                     TVRVSRPFDAETPVREFGSWLTPNERFFVRSHFGPPGAETIQPEAWRLTVKGLVQNEL
                     TLTLSELRKFEQVTITAVLQCSGNGRAHHRPKVPGVQWERGAAGNAQWTGMRLRDLLQ
                     RAGVKPQGLHVQMQGADRPALPTVPLFTRSIPIAKAFHPDTLLAYEMNGRPLPLLHGA
                     PLRVITPGWMAESCMKWLTELTLRADETPGYYMQQAYRIPETSIQPGSGLPGSVMVPV
                     EQMPVKSLIAAPGEGDTLKAGPVTIQGVAWAGESPITKVEVSCDDGKTWEPARLLGEA
                     QPYAWRQWQYLWNARQVGPTTILCRATDAQGAQQPEKSPWNPGGFLWNGWDRLAVTVA
                     A"
     gene            complement(25253..25651)
                     /locus_tag="PK589_11800"
     CDS             complement(25253..25651)
                     /locus_tag="PK589_11800"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNJ20333.1"
                     /translation="MKLSVAYQGGTRYDILSDRHRVVTDQPTEDGGTDAGMSPVELFV
                     GSVASCVGYFVGQFCARHDISREGLKVDAEWAVAEGPHRVGQIQLAIRLPHRVTPEQK
                     ERLLRVAHACTVHQSIMVPVQVAIELNPHT"
     gene            complement(25735..26580)
                     /locus_tag="PK589_11805"
     CDS             complement(25735..26580)
                     /locus_tag="PK589_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ20334.1"
                     /translation="MRHVLFSVIVIVGLTAGCVESKDTRGAAPSAVSQSDSARLDALF
                     SHPSPDSIPGGQRGEQIRLGYELVVHTQQFAAPYVGNRLTCANCHLDAGLDPNSASFV
                     GLSRAYPEYRARAGKVLTLADRINDCFERNLNGKAIPQDSHKLQAIVAYIEWLSKDVP
                     AGSRMAWRGVPTLSSTKPPDPANGAKVFTARCAFCHGADGQGTMAAPPVWGAQSYTIG
                     SDMARVSVAASFIKQNMPRTRGWALSDQEAYDVAAYINSQPRPDVAGRGRDWPKGEKP
                     ADAPN"
     gene            complement(26822..27178)
                     /locus_tag="PK589_11810"
     CDS             complement(26822..27178)
                     /locus_tag="PK589_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ20335.1"
                     /translation="MGMRIWGTLLCTVGVLLGSMPISLAASGKGNPATGKKLYHESCQ
                     NCHGPAGKGDSEMAAYLTPAPADLTSKTTQEKTDAQLRKVIQEGRPGTAMTGYAAAFD
                     EEKLNDVMAYIRSLKP"
     gene            complement(27211..28536)
                     /locus_tag="PK589_11815"
     CDS             complement(27211..28536)
                     /locus_tag="PK589_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNJ20336.1"
                     /translation="MPSRLYDWLDARLNLKPVQRTLLDEPIPGGASWIYVFGSVTLFL
                     FVLQAATGMFLALYYAPTPDHAYDSVRFIETDVTLGWLVRGLHHWGASSMVVAIGLHM
                     LQTFLYGAYKAPREVMWMVGVVLFLIVMTFAFTGYLLPWDQTAYWATQIGINMVGTVP
                     LIGDVASRILRGGEILGALTLSRFFAIHVLFLPAMLITGIAVHLFVLRRVGPAGPWTD
                     DRASLGAETFAPRQVYMDAVVIAGVFLVVAALAFSIPLPLTDKANPSDTSFVPVPEWY
                     FLFYYELLKYVHGPLEPLATWILPLCVILVMLFWPFIDRNPVRNPIRRPVALGSGLLF
                     LLVVFGLLGISIRNLYAVPRVDPAVARGRAVYAQLGCVGCHRIHGEGGAVAPDLSTVG
                     DTRPDREWHLKHFRDPQSVSPGSFMPKFPLTDQQLNDLTTYMLSLKRAT"
     gene            complement(28595..29149)
                     /locus_tag="PK589_11820"
     CDS             complement(28595..29149)
                     /locus_tag="PK589_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNJ20337.1"
                     /translation="MVSHAEQPDQQPEGGFSTPVGSRRTFFQWVTLAAAGLVGVGLSI
                     PLLSTLISPAFHRRRRDWVDVGAVDDLSSGHPKQLDHVTTIRDGWLETTSHKAVWAVK
                     QAQGDIKVFSPICTHLGCGYRWDETDKKFLCPCHGSSYDVNGQVLGGPAPRPLDTLPS
                     KVEGGRLLVMYKDFKSGLPTSVEI"
     gene            complement(29184..>29404)
                     /locus_tag="PK589_11825"
     CDS             complement(29184..>29404)
                     /locus_tag="PK589_11825"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNJ20338.1"
                     /translation="MACNVGGVERPVRIVLGILLIGIGVFGALPPVGMGIALAAGTIA
                     LVTGALGFCPLWSLFGINTCSVGAGKKA"
     assembly_gap    29405..29504
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29505..29812)
                     /locus_tag="PK589_11830"
     CDS             complement(<29505..29812)
                     /locus_tag="PK589_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20339.1"
                     /translation="MNWRIFGWGIAALWMITIGIMSTFLVQGHTRPGDDGRTEIILAP
                     SERDLVLAEMRGLLKAIHGVVTVLGAGDQPLKAAESSARAAGMAMAADVNPAIMLKLP
                     L"
     gene            complement(29832..30197)
                     /locus_tag="PK589_11835"
     CDS             complement(29832..30197)
                     /locus_tag="PK589_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ20340.1"
                     /translation="MHLRIGVIAVSQLILLSAIVVAVYAGPGNVGKGKTLYANLCIRC
                     HGVDGKGKGEMTFTPPVADLTAPAAQTKLDATLLKTIHDGRRDTAMGAWKFVLSEEEM
                     RDVTAYLRQLGSEAGRGRP"
     gene            complement(30411..30845)
                     /locus_tag="PK589_11840"
     CDS             complement(30411..30845)
                     /locus_tag="PK589_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNJ20341.1"
                     /translation="MDVRSKVYTYRTAVQWTEQKKGTISSEGKPDIQVATPPEFKGHA
                     GIWSPEDLYVAAANICLMTTFLAVAERGGLAFTAYHCEAEGRLELVEGKFQVTVITLR
                     PHITLSPGGDASKAKELIEKAEANCLISNSMKTRIALEPTIV"
     gene            complement(30983..31396)
                     /gene="rnk"
                     /locus_tag="PK589_11845"
     CDS             complement(30983..31396)
                     /gene="rnk"
                     /locus_tag="PK589_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HNJ20342.1"
                     /translation="MNPRNICMTAVDLSRLRDVLRARTSVGRDRDHLDSLEEELDRAD
                     VVDPSAIPPNVVTMNSRVRIEDVDTGMENVYTLVFPSDASIAERKISILAPIGTALLG
                     SRVGSTVDWPVPAGRRTMRIKDVLYQPEAAGDYHL"
     gene            complement(31393..32373)
                     /locus_tag="PK589_11850"
     CDS             complement(31393..32373)
                     /locus_tag="PK589_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ20343.1"
                     /translation="MTTPPFVKHILFATDFSDDASRAQEYAVHLAGACDAKLDVLHVI
                     EAPHEMQADAESLGIVGRSRDAAARQLEEVRDELARRGTPARVRLLLGNPSEHIHQAA
                     REQNIDLMVLGAQGRTNVLYGLIGKTAERLIHEGPCPVLAVPGSRQDAVEMPPTDRPA
                     LIRHILAPLDFSTPSLDAVEYAVHLAKGVGATVTLMHVLEPASYDLDCGLGVIEEESS
                     KRDYWNRQLMDLRDVVTAFDLQADVALCGGVPSDAILASALRQHADLIVMGTHGRRGI
                     SPDRCGSVAEAVLRRATCPVLTVKTGKFDPGHRRVVPNALERHVTRGENA"
     gene            complement(32710..33729)
                     /locus_tag="PK589_11855"
     CDS             complement(32710..33729)
                     /locus_tag="PK589_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNJ20344.1"
                     /translation="MREEGDHPGRRWAIILAGGDGVRMQPVVKRWLGRTMPNHYCRYV
                     GTRSLFQHTLDRMRYVTSPARTLVVVGRGHQEAWAQIGQRHPGMVLVQPRNAGTAAGL
                     FLPLTYIRARDPAGMVVVTPANQFICPDERLPAEILYAVRAAEKLPANLVGLAARPDG
                     PDSEYAWIKSGHELVRLGLRSIRAVNTFLHRPEPDLARLAYASGALWHTGIMAASVET
                     WWNLGRRVLPEMMRLFERLGEVINTPREPQALRDIYARMPVRSFSRDLLAAVPGVAAV
                     MEMQGVWWSDWNHPERIMKTLTRLGRDTASVMPHLRAAVAERQSAQTLDIAMAHRTDG
                     WSAEG"
     gene            complement(33749..33904)
                     /locus_tag="PK589_11860"
     CDS             complement(33749..33904)
                     /locus_tag="PK589_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20345.1"
                     /translation="MGVMPRGMTERKGDAMKQVISLAPVMLLMVLVMGAMGIMVFWGL
                     SLLDLSE"
BASE COUNT         7171 a        10542 c         9253 g         6886 t
ORIGIN      
        1 actgttcctg ctgcacatac ccgtcaccgc gcacctcatg cttcgcagcc tgctcggatg
       61 gagtcttggc gcaggataag gtaaagacca aaagaatcag agcgcagcag tgaacgaccc
      121 atcttgtcat cggaaacctc caggcgagaa ggtacttcag cttcgccgag gcatcagtca
      181 ggcgatatgc caggagagcc tccctcctgc cgaacgacct cagacctgat cgcttgccga
      241 tagtgtaaac ctccctgcca ccctccgccg ccacccgcca aagccgacca ttccagaggc
      301 gactaacaac agcgtacctg gctcaggggc ggcatcaata agttgttcct tgactgttgc
      361 tgcaaccaca aaaggcgtcg cagttagtgc gaacctagtt ccaacaggct gatccgttat
      421 ttgtaacagg ttgagcgcga agagatccgt tctttgtact gttcttcccg taatctgggg
      481 atcattgtag gcgactacac caactgacct tccaagtggg ctgttaagaa taaatgtatc
      541 gaccccaaga actttagaaa caacagcacc tgatgcatcc agcaatttgg gcgaaaaaat
      601 gcttggggct ataaaggaga atgagcccgg agatggtcca gcaagcccaa tggtacgttg
      661 gacattagct tggcctagag ccataacttc ttgggtaaca tttttatctc caacggatat
      721 tgacttgacc aagagagcat cggcaacgaa tggatcacct actcggccag aattgagtgt
      781 ggttacgaaa cggtcggcgg cggcgaggcc tattgtagcg ccaattcccg cccccacagc
      841 ggttcccaag ccgggaacaa aggagccggc gatacctcct aaaatcccac cattcacacc
      901 agttatcgca ataaaccccg ccttggctcc tgctagacca acccgagagc cggcagcgag
      961 ttccgacgta aaatctgcgg tcacgagtcc cccagtgcta gttttcggaa ggatgaagtt
     1021 gccatcgtta atatttgcga tgaagttgtc acgaagttgg tctagcgaga accctgcagc
     1081 ataagaagac tgccacgtgc aaattactac acagaatgtc actgctatca ctaggaagac
     1141 ctgagacctt ttcataactc ctccttgtga atcaaaatcc aagctttgga attcatgtca
     1201 tctgtcattt agcaatggca gatgaccttc cgtcacaata atgtcaggcc tcaacgtctt
     1261 gacctccacg agcaacaccc ggccctcctc aacagccccg acgacacaat aatcaggctc
     1321 gaccaactgg acaaaccggt aaatcatccg tgatgaggag acgcggtcta cgccgttggt
     1381 gatcacactc caacatgggt tcattctgtg gatcaatgat gcgttatgga tcctggggca
     1441 aacatagcgc gaagcgcaga tggcgtatgt cgccagcttg cttacgtagt tggaggtgtt
     1501 accaatgcga tcggcctttg ggccgacacc agctcttcgg caatcttgcc tacctcgtcc
     1561 attttccctc cggccagata cagatcgccg agggaccatt tcgcctcgaa atgcgacgga
     1621 tcaagttgca ccacccgacc gagcgcctgg tacgctgtgg atgggtctcc gccctttaac
     1681 gccgccttcg cgagtttcca ctggagagaa ggattatccg gagcagcccg cacgacattc
     1741 ttaaactcga tcaccgcttc gcggaactgt tcctgctgca catacccgtc accgcgcatc
     1801 tcatgcttcg cggcctgctc cgacagtggc tcagagcagg aggtcacgag cgctaccagg
     1861 tagagagcgg gtaaaaccct gacccatgtt ggtttggcga acacttttag ggctcgcttg
     1921 gttttttcca ccgccaagca agaaggctga gggcgccggt gccgatcagc aacatcgtac
     1981 ccggttcggg agcacccgcc gtgggaacgg tggcgaatag acttacagga gccgcgaagc
     2041 tacttcccgg tgcgaagatg tctccctgca atagtccggt cgtaaatgtg ggaccgttgc
     2101 tagattgaaa gccgaataac tggcttgtga aaccaggggt caggccctga ttgaggaatg
     2161 ttgaataggg atgggagcgg ccacctaagg gattggacaa aatttctatc cgctcagcgc
     2221 ctgagactgt tcgcgctcca attccaaagc tatgagtgct ctgaagatta ttgccgctcg
     2281 cattcacgag ggagccgccg ttgagaaaat caaggcgcag gtctcctgac gcgaacgatc
     2341 ctgtcgaggt tgcagacaca ggtgtcacaa cggacatttg cggctgaaag ctaccccata
     2401 tcgcatggga agaagcggga ttggcaattt gataaagata ggtgtacttg ctggaatcaa
     2461 accctggatt acccgcggcc gggacataaa gttgagataa catactgccg aacgggtcga
     2521 acggcggcag gacagcataa ttcaccgtcg ccgatatcac aggagatgcg gaaaacggca
     2581 catcatggcc tgtaaatccg tacagcggaa cagcatgggc agttgtttga gcaagtggcg
     2641 cagtcaaacc caagatagcc acacacatga gggacaggct taatttcttc caattacatc
     2701 gattcatgaa tctcattccc ttcccacaca aatccatttc agccttacat gagcagcgcg
     2761 tagtgcagcc aatgcacccc gtgcagcatc gaccatgctt ccgaacgcgt cctttcgctg
     2821 tgggcgcccc ttgtactgtg tgcactctaa ggggagtgcg caagtttggg aactagggag
     2881 gttcctcgcc atagcatgct agcaaggagg catagaagga aaggagtggt tcaggctgta
     2941 tctcccgtcc acgactcgat tgagctctcc taactcctat gcacggaccg taactgcctc
     3001 cactgccaga tgaccagata aacaaatccg gatgcgatga gcaacagcgt cgaaggctcg
     3061 ggtgctgatg ccgccgaggc gacaaccgtc aacgaaaaac tgttgcctcc gttcgtgagt
     3121 ccgctgagcc aagcaagccc cgatgccagg gagggcagcg aaacgccgcc tccaaataac
     3181 gtgccgctat tcaatcccgt gcagccgagg gagcaggtga gcagactaaa ggtgttcccc
     3241 aatcccggtg taaatccatt gatcaatcgc acggaaagac tgccgccgaa cgtccctgaa
     3301 cctgtgactt tcagcacatc gtattgagat ccggccagca agccaccgat cttcaagtcc
     3361 aatcttccat aaatgccttg ggtgtagttc gccagcgtca gcgtccccgg cacaagactg
     3421 gtccccggtt gaactacccc tccagtgttc accactgcac cggtaatggt gcccgttcca
     3481 ctgaggctac ccgcttgaag gctgatcgta ttgccaaatg ttccattgac gatcgtgtga
     3541 gccaccgaag tagtttgcga ataactcccg gttccagaaa ctcctccaac agccccgaca
     3601 ccagccccga cctcaaatgt tccagaattt accactgtgc catgattgat cagcgtcgcc
     3661 actccattga ccgctatctg aaacgttccg gaattcacca cgctgccatg gttggtaaaa
     3721 gtccccgcat cgacgcgcgt gctgcccgca ttcgtgaaga tgccgctgtt gacggtcgtg
     3781 gatacgaccg gaacgaactt gttgaatgcc tgaaggtagg ttcccgtcgt ccccacggtc
     3841 atgagcccgg cgttttccac acgagcacca ccgacctgaa acgtaccgta gatttgtgtg
     3901 gtgccgctgg cttgctggat atactgacca actggtggtg tccgattgga gaagttgccg
     3961 aacgacgtcc cctcaccgat acgcacctgg cccgcgttgg caaaagtgcc gctattggag
     4021 gtcgatgcga ctggggcccc ggtaccatct ccccgcgact ggctatatgt ccctcccgct
     4081 tcaacagtga acatgccgga gtttgccacg ctgccttgat tggtgaagac acctgtacca
     4141 atgtttgtgg tgcccgcatt ggtaaaggtg ccagtgttag tagtgcttcc ggccaggatc
     4201 ggaccaggaa agccttcctg acgatactca ccagttgacc cgaccgtgat cgtccccgcg
     4261 ttgtgcacct caccgcctat gttctgaaac ctcgcatcga tttgagtcgt gccggttgaa
     4321 gcttgctcat accgcgagac agccgcacga atactaatcg gatcaatgtt gaaatcaccg
     4381 gcggcgatgt ggatgcgccc cgcgttcgta aatggcgcgt cgggggtaat cgtcgttggc
     4441 gttacgaacg aacccacagc ctgccgctga atatattggc ctgtttcgcc cactctaatg
     4501 aagccaaggc cgtgagtgac ggcgccttcg ttgacaaagg tgcctccaat cccggtggtc
     4561 ctatcgccgc cgttatacac gccactcgcc aggttaatga attgcgatcc ttcaccaaga
     4621 cctaagaaac caccaccagg gttggggaag ggacgaacaa cttcggtaaa cgtacccgaa
     4681 ttagtgatgg tgcccctatt gttaatgacg ccggaattgt tgaactgatt cgcattgtga
     4741 aacgaggcaa gcggctcgat attgatcgtg ccaacgttgg tgaacgggac aagattttcg
     4801 cgcggcgtgg aaggagtgat cgtaatggtg tttgcgtctg gattcggatt gggttcgatc
     4861 tgaatggcat gaagctcgcc cggagctatc atgagcaacg ccatcatcag caaacagtcg
     4921 atacaaacac gaatagcagc agagggagga atcatgttcg acctccttcc ggaccggctg
     4981 tcgggctcag cgctgaacgc acgagcgggc actctcagaa tccggtcagt accctacgcc
     5041 gaatgtggac aggtgggaac tgggaagttt acttgaggcc gcacaaacag aaaggtgagg
     5101 agtaacacca cctacaggcg aacaagtcct tcaataatgg catgttttgc caacgcgacg
     5161 gtggagtgga gattcaactg gtccatgatg cggaacttgt ggaactcgac ggtcttgacc
     5221 gcgatattga gcaacgtggc gacctccttc gttccccgtc cctccgcgat caactgcaac
     5281 acttcacgct ggcgaggagt cagagtagac actgttggaa ccttcctctt tggttgctca
     5341 ccgcccacat cctgcttcac cacatttttg gcaatgagtg gagtgagata ccactgcccc
     5401 atcaatacgg catgaatagc ttgagtcaat tcggatggtt gcgaacgttt cagaacatag
     5461 cccgaggcac cggctttgaa tgcctcagcc acgtgccagg agctttcctg ggcggtcagc
     5521 aagacgattt tactttcagg cacctttctg accaggggac gaagcgcgtc caatccgttg
     5581 agcaagggaa ggagaatctc catgacgatg atgtcagggc gcagggtttc agcctcgctg
     5641 aggagcgcgc gtccatcggc aacagtgccc acaacatcgc aatccggctc aagcaaggcg
     5701 cgagtgccgg ctgcaatgag cgccatctca tctgcaataa gtactcgagt cctgcgttgc
     5761 tgaaaatcac cttcgaacat gggtcttctc gtttagctga ttcgatcctg aagcgtttcg
     5821 gacgcagacg caactatagc gcagatagga cttcacagat gtcgcctctc ctcttacgta
     5881 ctgctatcca cgggatgaga aaacccacct gacaatcttc aggaacccga ccgacgttgt
     5941 gactccttca acgccgccgt gatgacggtt ttcgtgcgca gacgctcgtt cgccgccacc
     6001 gttcgcgcca aggcctccga ctgcgatttg ccatgattat cggtccctcg ctgcgacttc
     6061 tccatcgcac ctgtaatcat ctctttcttc cgtctgagtt tcgccgcttc cacggtccgc
     6121 accaacgcgg ctgaaatttt cggcgaggtt tccatcgccc cttgccgttg tgagccctcc
     6181 attgctgcgc tcacgtcgtc tttcttctgt cgccatttgg ccgccagagc tttgcgagcc
     6241 aacggactgg atgacgtggt gctctttttt atcttgccgg tcttcgaggg accttttggt
     6301 gtcgtcatgg tgttttccct cctcgtcagg actgggtggg tgtcatgcag cctcacagcc
     6361 gcgccacaaa tatcccacag tataccaagc cattccgtgg agggggcacg ctgacacctc
     6421 atacctcatc gatcaagacg catgacagtc tgtgggtcga cggcattgtg ttccacctat
     6481 cgacgcggca tactgagcct gcctttactc tctgcctaag gacaccacgt gatgctgaca
     6541 cgactctgtt gtgctggatt ggtcttggcc ctttgtttgt ccaccacacc cagccggact
     6601 ctcgctgagt cgggaggtac gggcgctccc ctgaagggtg acgccgcgcg aggccaggtc
     6661 ctctttaacg gcaagggcat ttgccattac tgccacggcg tcgatggagt gatcgacaaa
     6721 aaaccttcac tcaaaccaga gaccgtggcc gcaattgcga agcacgctgc ttcggcaccg
     6781 gatctgcgca atcgtgcggt ccacacgcta aaagacaaca aagcccgctt tcgggcgatc
     6841 cgtgaagggc atccgggaag cggcatgttt cccgactcca cgctaagcga tcaagagatc
     6901 acggacctct tggcttatct tgcggcactc aggcaaagcg cctctgctcc cgccaaaagt
     6961 ccatactaat cgtcgttggt agccttaccg gaacaccgtg cacatcgcac atgaccactc
     7021 tgtgcggaaa cgttttcgcc tgtgccaacc tcagaccgct ctcgcaatcc aacgggcttc
     7081 aacttaaccg gtggagatgc cgggagacat cgtgatagcg cggcggcgtg acgagataac
     7141 aataatcgaa gagccgatcc gccgcctccg ctgaaattcc tgcctcccgt gcttgaagca
     7201 tctccataaa ccgattcatc gtccggtaca cagcttcgct ttcgccgttg agcagatgtg
     7261 aacgcacggc cgccagctgt gcgacatacg gcgaaagaaa ttcttccgca ctcccatctt
     7321 tcacctgctc ggcgagcacc gcctgcacca tcgtatcgag ccaatcctgg tccccctcag
     7381 ccatcacagg ggcaacaccg gagaggagaa cggtcgccat caagatgcat aacgacgcca
     7441 tccgcgttat gtctcggctg attcgcatag tcacctcccg ctttgacgag ggctccagta
     7501 gcactacgaa tactgaacgc agcataggtt atgccaaggc cgagtacagc caccagtaca
     7561 cgcacagccc ttcggacacc gagaacagac gcacacacaa ggagttggcc atcgaccgga
     7621 tgggaatagg aaggggtggc gctctggtct tcatggggct gccctattac gcgacagcga
     7681 aggatgtgct ctgtggaagg gcccttcatc gagggccacg gcttgaccgc tgccgtacca
     7741 gccttacctt ataagtgagc ggagggttgg ggtgagagga acggaaacgg tatcgggatc
     7801 gagtgcaggg gtggtgagca ggcccgcagg tgcgaagcgg gaagcctgaa acccctccaa
     7861 gatctcaaga gttgaaagac cctctctgga ccaacctccg ccggtttatt tcttttcttg
     7921 gccttctttt caaaacctca ccgcgacagc tggcgcatcg ttcacaaggt ctccttgcct
     7981 caacggcttg tccacttctg ctgaggatcg gacccagccc atctccaagg cgaattgttg
     8041 cacccggctt cgcggcgcaa tcggtgtcgc atctcccctc tcgcgatccg ttgggccgca
     8101 cggatcaatc ttgccggtta atgtcggcca cgacgttgcc ctccggatgt tctagttcaa
     8161 tctggccttg tcccgtttct ccaacagctg cctcgcgcct tcgggcgtcg ccatctttcg
     8221 ccgatgttcg agaatcagcg agtcgatgtg ctcgccgcac atcacgcacc gccatcccat
     8281 ctggttgacc tctcgaatct cctccggcac catgaatccc ccgcaacgta gacatccgtc
     8341 ccggacaata tcggtctcat gagtcgtcat atatgctctc acctttctgt tgagcgtgat
     8401 atggtcgccc tgcacatggg aggacgaact gatgaaaagg attcagcaga cacgggtcga
     8461 gccctgcccc cacaatgctg aaccacactc aaacccactt gacgtggacg gcggcctcgt
     8521 tcaagttggc acatcgcaac catgacacat cctccttcgc ccccaaccga cacctgtatc
     8581 ctgaaagtcg tcgtcggctc acatgcccac ggcctggcca gaccggatag cgacaaggat
     8641 tttcgcagcg tcttcgtatt gcccaccgcc gaattgcttc gtatcggctt caaatatcaa
     8701 ggcacgagga tgatgaagga ggaagatgat gaaacggcct gggagatcgg ccactttctc
     8761 caacttgcgc tgcaaggcca tccgttggtg ctggaaacgc tggtcgcccc ggtcgtcgcc
     8821 atggatgatt ggggagcgga gctgcgccaa ttatttccgc gtctctggtc ggcgcagaca
     8881 gcctatgatg cctttctgaa ttactgcgac aatcaacgaa aaaagatgct ggagaaaaaa
     8941 gacagccgtc ccgaaaaata cgccgcagcc tacgtgcggg tgctcttcaa tctgtgtgag
     9001 ttgttggaac gaggaacctt cagcatcagg atctccgaaa ctccggtcgg tgaagccgtg
     9061 gtcaaaatca aggccgggca atttcgcccc ggagaagtga tcgacatggg agaacattgg
     9121 agagcagaag ccacccgccg gctacacacc tgctctcaaa aggcccaccc tgaaatagcc
     9181 gatgccttcc tcctcagggt tcgcaaagcg ttcctcacat aaatccacaa cctctccccc
     9241 tcagatccta gacatccggg caatcgggac gtacccctgc cccgccaaaa aagaacccgc
     9301 ttccgtgtag agatcaggcc tgccgaccga gcgagatcca tctgaacatc ccggcacaac
     9361 tctcataccg gatgttccgc gcctcccgtt catcgggctc catgggagcc gtgcctcata
     9421 ctggcaccga gatggaagcg cgtcccttca gaactgatcc cgaaaccggc acaggcgcaa
     9481 tccccgaacc aactcttcgc tgcacggaag cggggatgtc gaccttatcc tggctccacc
     9541 tcccttcaag gatctagcta agcatggtcg cctcgaaatc aatgaggtaa ttggcaccat
     9601 tgccggtgcc tgccgctgtc ccgtatcgcc ccactggcag gaccatgtcg caggcatttt
     9661 tctgccaggc gaatggactt cgcggctcca ggaaggccat acgttcaccc ggcattctgg
     9721 catcgtgcct gcaatactcc gaggcatgga gacctgtggg actcatcgaa accaagaaat
     9781 caccagaata gataaaggag tacggccatg agcggactga ctcgctggga accatttcgc
     9841 acacagatga atccctggag agaattggaa gaagtccaga aacgcctctc atccatgtgg
     9901 ggcggagcgc cgcagaagga aggaagccag aaggagtcca tctccgtcgc ggaatggtca
     9961 cctctggtgg acatcaccga agacgagaaa gagtatctga ttaaggcgga actgccggag
    10021 atgaagaaag aacacgtcaa gctgacggtg cacgacaatg tgctggccat ctccggcgaa
    10081 cgcaagtacg agaaggaaga aaaggacaca aaatatcatc gtattgaacg cgcgtacggg
    10141 agtttcatgc ggagtttcac cctcccggaa gatgccgacg gcagcaaggt ctccgcagaa
    10201 tacaaagacg ggatcctcaa tgtgcatctc ccgaaatcgg cgcaggtcaa accgaaatcc
    10261 atcgaggtga aggtctcctg aacgaaagga gcgctcatgt ttaagcatcc tcgctatctg
    10321 gatattccgt gggaaatgca ttggcacccg gaggaaacca gtcaccactg gtggttgata
    10381 ctgctcgtga tcatcgcggc cctgtttttg atcggcgtcg gggtgacgag cggattgtgg
    10441 tgactcgact cacggtgtgg ccagttgatc ggccagcgca tagaggaacg gacggtcatg
    10501 tttaaaaacc gtgaagaggc cggtgaactg ctggcacagg agcttgtcgc gtttcgaaac
    10561 gcccccgacg gcatcctgct cgcgcttccg cgcgggggcg tcacggtggc ctatcaactg
    10621 agtctggccc tgcatcttcc gcttgatgtg ctcatcaccc ggaagatcgg cgcgcccggg
    10681 aatccggaat atgcgcttgg cgcggtcagt gaaacaggcg cggtctactg gaatcaggag
    10741 gccctctcag gcctttccct ttcagagcgc gacctcgcgg tagccgtcca cgcgcagcaa
    10801 atagaagtgt cccggcgggt ggcgctatac cggcaagggc ggcccttccc caatctgaaa
    10861 gaccggaccg ccattattgt ggacgacggc ctcgccaccg gcgcgacctt ttttgcctcc
    10921 gtcgcaaccg tgcgccaggc ccgcccgcgg cgcatcatcg gtgcaatccc ggtcggcccg
    10981 cgtagcacga tccaggaagc ccgcagtctc gtcgatcaat tagtggtgct ccgggttccc
    11041 gagccatttt acgccgtcgg gaatttctac cgggattttg agcaggtcga agacagggag
    11101 gtgttgcagt acctcaacct ggcggaggaa gcatttctct cgaaatcgta agtctggagt
    11161 tgccgtaacg gttggcagac gagccaagaa agggactgga catgatgacc aaccaaccag
    11221 aaggcggtca cagcgcgcgc cgcgatcgtt tcgtcgaaga atataaacac gattcataca
    11281 agatgccggg caagctgaaa gagcctgccg tctgcaagac ttgcggtgcc ttgtatcaca
    11341 agggtcgatg gacctgggag atcaagccgg ccgacgccga acagatcacc tgcccggcgt
    11401 gcctccgtat ccaggataag aaccccaagg gcttcgtgac gttgaaaggc ccctacaagg
    11461 ctcagcatcg tgatcaggtg atgggactta tccgcaatac cgaggcccag gaaaagaagg
    11521 cctatcccct ggctcgtatt atgacgatcg aggatcggga ggaaggtctc gtcatcctca
    11581 ccaccgacgc ccacctgccc cggcgcatcg gtgaagcctt aaaacattcg caccatggtc
    11641 acttggacat tcagtatgac caggatgaag actttatccg gatcacgtgg accggataga
    11701 tgccttgaat gaacggatta tgcaccgtgc cagaagtggg cagatgaatc acaacgaggg
    11761 aaccatgggc gaacagcgtc gaggatggag tctagctagc gggttgctga ccggtggaat
    11821 cctggttctg gtcgctatgc tgccgatcgc caaggcagaa gtccaactgc tgcccggcgc
    11881 agccgctgag ctcgatcagg cgacggccaa cgcggtgctg aaaacgtttc agcaggcgga
    11941 agaggcgctt cgggggcgcc atgtcgatgg cgtgatggcg ttgtattcgg agcagtacga
    12001 ctatcacgga ttgcacaaga aagacatgcg caaagtctgg gtggatttgc tggatgaatt
    12061 tcaggatctc gccgatgtcc atcacttctc ccgcatgagc aagatcggct ctggatcaaa
    12121 aaccatcatt gaggtcacct gcacgggcag cctgacaggc ctctccaaaa ccggagggct
    12181 gcgcgtgccg atcgacagct ggtatgagga agtgcactac atgacttggg aagacggaca
    12241 gtggcgcatc cggggcaatg tcggcgattc accgcgattc atgcccttcg gcacgtcacc
    12301 gcatccgttg ttttagcccg catggtcatg acgatgcatc gagacatcgc cgagtcacgt
    12361 tgcgaggagg ggcgcggaaa cggaagaggc tgggggtacg cttggagtac gctgagaacc
    12421 agaggggcga gccaagcagc caaaggcccg tcgcagcagt gactcggcga gtgcagcagc
    12481 agtgctcatg aaccatgcgg gctagtgaaa ggagtcctat gcgcgtcctg atcgcactcg
    12541 attggtcgga acaggcgttc gcagccgttc gagaagtctc gtacctgtac gatctgcagg
    12601 aggtcgtcct cgtacatggc atcgatctgg gcatgtttca atatccgatc gtcgcggaag
    12661 tgagcaacat gcaaggctac gacgagttcc gcaaggccat ggaagcggct ggtcaacaat
    12721 tgttggatca tacctccacg ctcctgccct cccaaggcgt gtcaaccacg cgcgtctgcg
    12781 aattcgccaa accggcgtcg ctgattctcg acaaggcgcg agacatgcgg gcggagctga
    12841 tcgtgatcgg agggcgtggc agaagccgcg tcggggaatt cgtcctgggg agcgtatcgc
    12901 accgtgtcgc gctgcacgcg gattgtacga ccttgatcgt gaaggaacgc gagggcccgg
    12961 tcaagcgagt gacggtggcg atcgaaggcc atgaggacgc cgagcgcatc aaagcctggc
    13021 tgctcgcgca tcccttcaaa aatcctgtcg atcttacgat cgtgagcgtc gtgcggccaa
    13081 tcccggccac ggatccgttc aacctgtttc ctctccagga ctggaccggt atcgccgtac
    13141 gatcggctga ggacttggtg aagaatctcg cagccgctgt catgaatcat cggtactcgg
    13201 tcggcacaca agtggccgtg ggcgacccga ctgatatttt gacagagcgc gcccggtcgt
    13261 ccgacctgtt gatcatcggg tctcacggga ggaagcgact ggaacgtttc ctcctgggga
    13321 gcatttccca tgcgctcttg caccaagttc cttgtcccgt gttactggta cgataaggtg
    13381 atggccgtcc ggtggcgaaa cgttgaacca taaaggagcc agcgcatgaa aacgttcagc
    13441 attctctgtg ccctcagcct tgtgattctc ggaagttcct gggccgccgg acagaccaac
    13501 cggggcaatc cgcgcgacgg acaggccatc tacgaaaaaa attgcctgcg atgccatggc
    13561 gaaaagttgg acggcaatgg gccggagggt cagtatctca tcgtccgccc ggccaatttt
    13621 caatcggtga actcgcgcgc caagaccgat tgggaattgt tgatcaccat cgcaaacggc
    13681 gcgttattca gccccatgca cggctaccgc gggaaactga cggatcagca aatgctggac
    13741 gtcttgtcct atatccgctc cgtcgccacg ccggatttcg tcagctagcc gggcagcctt
    13801 ctcggtgggc gatgctcgac tgacattccc cttgggcggg gcagcggaat tctggaacgg
    13861 cgacttgcaa ctgtgaggtc gcacggtcat gatcggatcc ggcgtcttca tgcttctgac
    13921 cgtggctcga cgcgctggta gcccatgcaa cgtattcgcc cccgccaaat gtccccgctg
    13981 cgggacgaag gtcccggttc ggcccacacc atcctcagcg atggtacgac ggccctcctc
    14041 cgcctggctc aaccggtcga tgcggaggaa ctgcaacgtt tcttctccca cctaaccccg
    14101 gccgcgacac ggcatcgctt cttctcggaa tccgctccgc ctgccgaggt cattcgcgcg
    14161 ctctgcgatc cttcgcaccc ggaacgcagc ctgaccgtga tcgtcctgcg ccggcacgac
    14221 gatacgctac gtatcatcgc atcaggctct taccacgcgc gaggtccgca tgaagcggaa
    14281 gtggcaatgg ccgtggacga cgcgctccat ggacatggtc tcggcaccat tcttctcgaa
    14341 cgcctggcgc tgttggcgat ccggcaaggg ttcaccaaac tctgggctat cacccatgcc
    14401 gacaatctgg cgatgcgcga agtgttcgcg acgtccggct tcaccatgga agaacatctc
    14461 gaaggcggtg acatggaggt cgagttgtca ctgaccccga ccgaccagag cgtgcggcaa
    14521 tccgaatggc gtgagcgcgt cgcgaccatc gcttctctcc atcctctgtt tcatccgcgc
    14581 gccgtcgcgg tcatcggcgc ctcacgcgcg ccgaggagta tcggataccg gttactcgac
    14641 gccctccatc acaatcggtt tgaaggccgc tgttatgcga tcaatcccca tgcgtccacc
    14701 attgccgggg tcgaggcgtt tcccagcgtg cgccaccttc caacaccggt cgatctcgcc
    14761 gtgatcgccg ttcccaaaga agccgtgcta tcggtggtcg acgaatgcgc tgccatcggg
    14821 gtccgtgcgc tggtcgttat cacggccgga ttcgccgaag tcggagcaga aggacggcgg
    14881 ttgcaggacc agttgctgga gaaagtccgg ctacacggga tgcgtatggt gggacccaac
    14941 tgtttcggca ttctgaacac ggatcccgcc gtgcgattga atgccacgtt cacgtctacc
    15001 tttccgtgcg cgggctcgat cgccatgtcg tcgcaaagcg gcgccctggg gctcgcgctc
    15061 gtcgccgcct ccgaacgatt acacctgggg ctttctacct tcgtcagcgt cggcaataaa
    15121 gctgatgtgt ccgtcaatga tctgctgcag tactgggaac aggacaccgc gaccaacgtc
    15181 atcctgttgt atgtcgaatc gttcggcaat cccagacgat ttgcacagat cgcccggcga
    15241 gtgagccgca acaagccgat cgtcgtgctc aaagcggggc ggacctcatc aggaaaacga
    15301 gccgcagggt cccacactgc cgcactggcc gccaatgatg tcgcggtgga agccctcttt
    15361 caacagaccg gtatcctgcg cgccgacacg ttggaggaaa tgtttgccct ggcgaccggg
    15421 ctttccgaac aaccgctgcc gaagggacgg cgtatcggga tcctgacgaa tgccggagga
    15481 ccggccattc tctgcgcgga tgcctgcgag gcggccggcc tcgaggtgcc tgaattgtca
    15541 caagccacta tttcccggct gtcgccgttt ctccccgcct ctgcctcact gcgcaatccg
    15601 gtggacttga tcgcctccgc tacccccgag caatacgaac aagcgatcgt cgcgctattg
    15661 acctccgagg atatcgatgc cctcatcatt ttatacatcg cggtcacggc gaccgacgtg
    15721 gaccccatcg ctcaaggaat tgcgcgagga attgaagccg cacgggcaca gcagctggtc
    15781 aagaaaccgg tctcgattgg atggatggtg gaaacggatc gcgaccgcag attttcgctc
    15841 ccgcacgaaa ccattcctac gtacagcctg cccgaactcc ctgcgcgcgt cctgggaaaa
    15901 gttgccggct acgtccagtg gcgcgatcgt cctctcggca tggtgccgga cttcgatgat
    15961 cttgacatct cgtccatcaa aaccgtttgt cgcagcgtgc tcgccacgcg gggaaacggc
    16021 tggttaacgg tcacggaaac acgtgcggtc ctcagcagca tggggatttc tctgccgcca
    16081 ggaggcctgg ccaccacagc tgaagaagcg gccacgatcg ccgcgcagat cggatttccc
    16141 gtcgccgtca aactggcgtc gcatacgtta gtccacaaga ccgaggtcgg cggcgtacat
    16201 ctcaatcttt ccagcaaggc tgaggtccgc cgggcgtacg acgaaatcgc cgcacggctc
    16261 gcgcaggacc acacgctcga cgccatggaa ggggtgttgg ttcagtcgat ggtcacaaat
    16321 ggcgtcgagg tgatggcggg gatggtgcag gatccttcgt tcggcccctt gatcggcttc
    16381 gggctcggcg ggattcacgt cgaaattctc ggggacgtac gattccggat cacgccgtta
    16441 acagaactgg acgccgccga cctgatacgc agtatcaagg gctatcgttt gctccagggg
    16501 tatcggggcc atgcaccggg cgacgtcgat gccatccagg agctcctact gaggctctca
    16561 cggttggttg aagaggtgcc ggatatcgtg gagctcgatc tgaacccgat tttcgccttg
    16621 ccgccaggcc agggctatcg aatcgtggat gcacggatca gagtagccgg aacgggctga
    16681 gactggcagg tgagaagggc atgctgcgac tcatgcaggc aggcggctcg attcagtcac
    16741 tctccgctat gacagagcat acactgggcg atgcgaccgg gttgatgggc cccgcgccct
    16801 ttgtcataca accgccggac tatcggctcc tgcctcagcg tcggagtggt gacgattcgg
    16861 ctaccgaact catgacacgc ggtgcaggag ttcgtccctg tcacgcgaaa gacgaattcc
    16921 ccatggggat tgcggcgtgt ctgttccgta atttgagcca gcatcccgcg atgctctgta
    16981 tggcaaccgg tacaggcttg tcgttgaaca acgagcggcc ggtgccatcc cgccacggct
    17041 gacgtggtac gagcttcgaa atcgccctcg ttatggcagc ctgtgcagcg cgcggaactg
    17101 ggccctcgaa acggctcatg gcactgcgta cacccgcctt gctccgcatg ataccggcta
    17161 aggtcccccg gggcccagaa ggccgcggga ctccgcgcgg tgccccaccc gatccacatc
    17221 accagtatca gcgtcgcaag cgccaccacc ggcactgcag ttgttggcag cggatgatag
    17281 cgcatggctc acgacattcc ggcaaagaac aaggctccca ccacatgcag ggcggtgaac
    17341 gccagaaaca tcaatgtcat cggtgcatga atcgcctgcc agagacgaaa ggcctcttcc
    17401 tgggaagcca tcccatgcaa ctgcgcgtcg acgtcacttt ccgacagtcc tcgatgcaga
    17461 aggtcatgcc gatgttcatt gagcgctcgc agactgaagg cgtgcaccaa ctggccgacc
    17521 acgccgctga ccaccacgat gaccatggag cccatcgcga ggatcggaac gatcgcgtga
    17581 tagtgggcgc cggagtgaat gaaaatcagc agcggcccga gcacccctgt caccatatga
    17641 actcgaaacc aaccacgcgg ccatcggtgt gagggattga cccgttttcg gatgggataa
    17701 acgaacacca gcagggtgat ggccaggcct gcccagccga ctccatgccc ataccctgtg
    17761 tgaccgaatg gcgtaacctt atccagactg agcagcgctt cgaacgcgag cgcgctcagc
    17821 atcgcagcga ccgacaccgt catgaccgcc aggaggcgcg accgcacacc cgtcatctgc
    17881 gcaatccgtt cttcatgccc cgctgattac cgtcggtact tctcaacccc cttgttgcct
    17941 ccagcatggc aacgtaaaca gtctgcgaac cgaccggggc catggatgcc cttcgctttg
    18001 gccttgagat cctgcaccag ttggttgtcc tgtacttgcg ggctgatcgc tccctctttt
    18061 ccggcccggt gacaatccag gcacctggtg gcgccagtcg cacgaaagat caaatcactg
    18121 tgcggattgg gcatcgtgcc ggcgttatcg gcaaccctga gcacaggcgc ccatagtccc
    18181 tcagatgatg gaatgcctac atcaggccat gcgtgttctc cctgcagcaa gcccgcaagg
    18241 acaaggaccg ccgacagact cctgagtgtc acggcgtacc gacggttcat gcgagcccgc
    18301 tcccgcaaca ggttcggtat cgttcctcgg ggatatcctt catggcctgg tataactccg
    18361 cccgctcctg atcggtgatg acctgatcga tcgacgggta gagaattcgc tcttccttca
    18421 tgttgtgcga cttcagcacg tcgaccagtc gctgttcctc cagattactg tcaggattgc
    18481 tcgcctgcac tttctggtga atggcttcca ggcactcgcc gatgatccga tgttcggtcc
    18541 gcatgacctg cgtcggtcct ccctctgcca tgccggagtt ttcttcccac tttggaaaca
    18601 agacatcttc ctcccacacg atgtgccgct ggagcccgaa cttgaattcg acgaacgctt
    18661 ccttcgcctt ggccatgtcc ttccgtttct gttgctggaa tgtggtgaag agcgcatcca
    18721 gccgatcatg atcctgttca aacgtgacac tgatcttctc atgggacatg ccgttctcct
    18781 tcgttatgaa tgacgaagcc tgtattccaa gtgacaggcg acacaggctt tgagcacatt
    18841 gttgaactcg ggcagaatct gcttcagatc tttcgtcggc atcacatcgg ccagtttccc
    18901 ggccgcggca tgatgggcca tcgcgagatc gtgataggcc ggcggaaagt ctcccggctg
    18961 ctgcctggcc atagcatacc ggtgcatgaa aaatcccaga tgcgattctg tcagtccctg
    19021 cgcaagacgg taatcctcat ccgctaaagc cgccacgatc gcttgaatcg tttccaaatg
    19081 ctggagcatg acggcgcgat gttcctgccg cgcctccggc ccaagaggaa tcggcgtacg
    19141 tacctcgggc aaatgcacgg gcctgacctc gtcagcagac ggcgccggct gctgcttcac
    19201 acacccggtc gtgaagagct ccatcgcaaa gaggaatgag agcccgcctc ccagacaaat
    19261 tttgcttcga ctcatgactg ccctctatcg cctgttgccg ataggcatcc accggcggga
    19321 tagtgcaccc ggcaaggcga tgtcgccctc cgcacggcgt tcgcacagcg accttctcag
    19381 gctctgtctg cccgctcctt cttgaaagct tcctttccag cggccaatgc ggcggcgatc
    19441 gtggcttctg tttccttcag atatcgcttg gcctcttcca tcgtgtgtgc aatgtccgtc
    19501 cggatttctt tggccttctc caacacatcc tcttccgtcc ggcgcgcata gcccttgatc
    19561 gcccggcggg tttcctcgcc ggaatggggc gcatacagga tggctgccat tgccccgagc
    19621 atggctccac tcacaaatgc gatcgagacg cccagcccgg aacaattgtg atcgtgatcc
    19681 gccatgatga cctcccctct ccccctctat gtttttagaa tctgagactt cccgctttgt
    19741 ctttatagaa agcagaggac gtgccaggcc agcgtgatgc gggagcagac aagtcacaat
    19801 tttgacaagg gtttcacagt gactgcgaca ggttgcgttc ccgcggcctg tccgtttggg
    19861 gaattacaga accgggcgag aatcaacagt ggcgcaaaac gcgccaggtg agagacgcgt
    19921 cgactttgga aggattagaa attctggcta tcagagctta ctgcggaccc accgcacaat
    19981 gtcctgaaga cccagcgccc ccggctgacg agccagttca cggcctccgc gcagaagcac
    20041 caatgtcggg atactggaaa taccgaacct cgcggccaac gtctgctctt cttctgtgtt
    20101 tactttggcc agccggatgt gaggttccag catctgcgcg gcctgctcat aggccggggc
    20161 catcattcgg caggggccac accaagaggc ccagaaatcg accagcaaag gaatctcggc
    20221 ccgctgcgcg tgcagatcaa aatcctccgc cgtcaaggcg atcggatgcc ccgaaaacaa
    20281 ggacccatgg cattgcccac aacgaggctg ctgcgccaga cgggccgcgg gcactcgatt
    20341 gatgctccgg caatgcgggc acaccaagtg aatcgattcc gtcacagcac aacctgcttg
    20401 ccgcaagaca ccgcgccatt accgatcaca ccgttcccct tcacagcgcc cgctcccgct
    20461 tcccatgcct cgatggccca tgccacccat ttctcctcgc atccccctgt gtcccatgcc
    20521 ttcgggcggc cccatttctc gaccctggcc atgctccttc gcgaacgtcc gttcatactg
    20581 gatcacggcc catatgtcct cgtccgtcaa ttggtcggca aacccgatca tgctggtgcc
    20641 cggtgatcca tgcttgatca cccagaagat ttctccatcc gtacgatggc gccagaatcc
    20701 gtgatgctgg aagttacgag gcgcaggact caactgggct gccaccggcc cattcccatc
    20761 gccttccgaa ccgtgacaat tgatgcaggt ccccttgccg gtataaagcg ccttgccccg
    20821 ctcgatcgtt tcggcagagt ccggcagagg atttctcagt tgccgcgcct cggctatccg
    20881 gtcggccgga actcgcggct gcatcacatc atgtcccatc gccatgggat cggaggacat
    20941 gactgattca gacgacacca ccggcatggt ggctaacaga ctgacgagaa tgatgaggta
    21001 ccgtttcaca gccaatcaga aatccagttc cgccatcttt cgatggaatc gcgtgatcac
    21061 attgggatcg ggcgtaagcc ggatttgcgc ctcatccttg cctctgtagg gaagcaggtt
    21121 caagacgtac cggagacttt ccagtctggc ggcctgtttg tcgttcgcct tcacgatgat
    21181 ccagggactg aaggtcgtgt gcgtcttgct gaacatttcc tctttgaacc gcgtatagga
    21241 atcccacatc tcttgcgcct tttcgtcgac cgggctcaac ttccattgct tgaggggatt
    21301 ctgccggcgt gcatcaaatc gtttggcctg ctcctctttg gagatggaaa accagaactt
    21361 gatgatggtg accccgtctt cgtacaacat gtgttcgaat tccgtgacct gctgcaagaa
    21421 gcgctgatgt tccttcttgc tgcagaatcc catcaccggc tccactaccg cgcggttgta
    21481 ccaactgcgg tcaaaaaaga cgatttcccc tttattgggc agttggcgga tataccgttg
    21541 aaagtaccat tgcccgcgtt cgtcatcggt cggcttgggt aacgcgacga cgcgcatggc
    21601 gcgcggattc agatgttcgg taaaacggcg gatcgtcccg cctttcccgg cggcatcccg
    21661 cccttcgacg agaatcgcaa tccgctggcc atcggactgg acccagcgct gcaagcgaac
    21721 cagttccacc tgcaactgcc gcaattcctg ctcatacagc agcgtcttac gcacctcttc
    21781 cagatcgaca ttcttactct tgatcaattg gatcagacct ctcggcgtat tgatgcgccg
    21841 gagatcgtct tcggtcagtg ccttccccgc ctcaccgatg aggccgtcgg ccatcccgtc
    21901 gccgttggta tcggccactt gatagccgtc gccttgccgc ggcacggcag tgggaatgat
    21961 ttccaattca tcttgcgaca ggtccttctg cgcgaacact tccgccggct ctgtccccgc
    22021 acgatctttt ttgccttttg attgcccgtc agctgccatg cgcctatcct cccgctacct
    22081 gcatcaaaag gtgacgttcc gatcacgttc agccacggcc ttgatatagt cgggcatgcc
    22141 tttgatcgct gctcctccca ccagatccgc cgcgccgatc tgccgtgcca ctgcacaggc
    22201 accacacacc cagatgggaa tctgtgccgc ctgaaccgcg gccaagagat ccttcaacgg
    22261 cgtcagattg acgccggtca catgatcggc tgtgcctttg cgccccagcg tcacggcctc
    22321 gttccacagc cacagcacca catcgtggcc ctgctccttg gcggtcttcg ccgccatgaa
    22381 cggcaaggtc gccatcgttg gatcgtccgt ccctcggcta ccggaaatca taaacgtcgc
    22441 catggaatcc tccgtcctcg aatcgctcgt cgagcggatc tcgtctctcg tcgttcgtct
    22501 ctcgtatttc gtctctcgtg agccgtccgg aacgcttcac gcatttcgtc tcaagcttca
    22561 cttattttgc caaacttctc acgtaggcaa tcaactgcca cacctgctca tccggcaacc
    22621 cggcaaagga catcatgccg gtcccgggcg acccgttctt gatgatccag aataattggc
    22681 catcaggcag atccttcatc atagcgccgc aggtaaagtt tctgggcggc gggatcaagg
    22741 ccgcgcccat gaacccctgc ccattgccct tttcaccatg gcacatcgcg caggccacgg
    22801 gctgggccgt ttgcaggaat aacgttttac ccgcctgaat cgtcgcatcc gaatgtggca
    22861 acgggctggt tttcgacagg aagtctgccg gcgccttcgc cgttctcctg ggctgcgggc
    22921 agacaccgct cggaccatca ctactgatgg cggcctgtgc cacatcggcg ccacctttga
    22981 aggtggcttt caagtaggcc atgacgtccg cgaccccctg ctgcccgatg gtcaaacccc
    23041 aatagggcat gttcggcgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    23161 cggcccaaat cagatcgggt gccttcaagt tgaaccgcga ctccgctttc ccttccaggc
    23221 ggtgacatcc ggcacagtta ttctggatca tctccttgac gcgagtctga tcactgccca
    23281 agagcaggcg ggggaatgaa aacagtccca ccaatatgac caacgcgcac ccgatcatcg
    23341 ccgtccgtct tccacccatg aaacacccct ttcgttcacc tcactgcgca cccggtcagc
    23401 gtgatgccaa ccaggcatgg atatctgcga cctcctgatg actcaagacc gagccccagg
    23461 ccggcatcgg gccccggcca tggagcaccg tctcccagaa gactccctca tttttcaaga
    23521 ttttattgcg ggccagtttg ggtcccgcgc ctcccatcgc cccctcgcca tggcacgcct
    23581 ggcagttccg cgcaaagatt tccgcgcctc gggccggcac cccgacaagc gggccgtcac
    23641 tcgcggtgct gggccctcca gccggagcgg tcagcgccaa ttcttcttcg atcgacggca
    23701 agtggtccgg cgcaccgggg tggtagcggg ccgtcacaaa ctgaagaagg gccgcggctt
    23761 cgtccttaga caagtccgcg ccccaatgca tcatcttctc caccgtgatc tgccatcgat
    23821 ccggcggtag cctctgttgc acgatcagat cggtcgtgtg gcacacggcg caacgggcat
    23881 gaattaacgt caccgcccgg cgagcctgcg ccggttcaat cgtatgcttg tcatcctctt
    23941 gcgccgccgt cccggcagca atgccgagga gcagtaccag tcccgcagcc aacaaagacc
    24001 atcgccctcg ctccctttgc gccccctcca tcacgcccct catgccgcca ccgtcacagc
    24061 caggcgatcc cacccattcc acaaaaaccc ccctggattc caggggctct tctctggttg
    24121 ctgagccccc tgcgcatccg tcgcccggca gaggatcgtg gttggcccca cctgacgcgc
    24181 attccacaag tattgccatt gccgccaggc atagggttgt gcttcaccca gcaaacgagc
    24241 cggttcccag gtctttccgt cgtcgcaaga tacttccacc ttggtgatcg gcgattcacc
    24301 cgcccaggcc accccttgaa tggtcaccgg accggctttc agcgtgtccc cttcgccggg
    24361 cgcggcaatg agcgacttca cgggcatctg ctcgaccggc accatcacgc ttcccggcaa
    24421 accggacccc ggctggatcg acgtctccgg aatacgatag gcctgctgca tgtaatatcc
    24481 gggtgtttcg tccgcacgca gggtgagctc cgttaaccat ttcatgcagg actcggccat
    24541 ccagcccggc gtaatcaccc gcagcggtgc gccatgcaga agcggtaacg gccgaccatt
    24601 catctcatag gcgaggagcg tgtccggatg aaacgctttg gcgatgggaa tgcttcgcgt
    24661 gaagagaggc acggtaggca gagccggacg atcggctcct tgcatttgca catggaggcc
    24721 ctgcggcttt accccggcac gctgtaagag gtcgcgcagc cgcataccgg tccattgcgc
    24781 gtttcccgct gcgccccgtt cccattgcac gccgggaacc ttggggcgat ggtgcgcacg
    24841 accgttgcca ctgcattgca gcacggcggt aatggtaact tgctcgaact ttctgagttc
    24901 ggacagggtc agggtcaact cattctgtac gagtcctttt accgtcagcc gccatgcctc
    24961 cggttgaatt gtctccgcac cgggtggccc gaagtgactg cgcacaaaaa atcgttcgtt
    25021 cggtgtgagc caggacccaa attcccgcac gggtgtttcc gcatcgaacg gccgggagac
    25081 ccgaaccgtg agcgggccgc tcacctgccc gatgacgtcg ctctgactgt cggcgaacgc
    25141 tctatcgacg ccgcccgcga ccacagccgc gccgaggccc tgcagcaccc gttcgaacaa
    25201 cgcccgcctc gtcactgcca tattcgccct ttcctcatgc ccacctcacc ttctacgtat
    25261 gaggattcaa ctcaatcgct acctgaaccg ggaccatgat cgattgatgt accgtgcacg
    25321 catgggccac cctcaacagc cgctctttct gttcaggggt cactcggtgg gggagacgaa
    25381 tcgccaactg aatctgtccg acacgatgcg gcccttccgc cacggcccat tcggcatcca
    25441 ccttcagacc ttcacgagag atgtcgtgcc gtgcgcagaa ttggccgaca aaatacccca
    25501 cgcagctcgc gacggagccg acaaacagct ccaccgggct catgcccgca tcggtaccgc
    25561 cgtcttcggt gggctgatcc gttacgacgc gatggcggtc gctgagaatg tcgtagcggg
    25621 ttcctccctg gtacgccacg ctgagcttca ttctgttctc ctccgtccgc atcccgcagt
    25681 ccgcatggtc gtgcgttcct caccggccgt tatcaccagt ggcatggtac gcgttcagtt
    25741 gggcgcatcc gccggcttct ctcccttcgg ccaatcacgg cccctccctg ccacatccgg
    25801 tcgcggttgc gaattgatgt aagctgcgac gtcgtaggct tcctgatccg acagcgccca
    25861 ccctcgagta cgtggcatat tctgtttgat gaacgaggcg gctaccgaca cacgagccat
    25921 atccgagccg atagtgtagg actgcgcgcc ccagaccgga ggggctgcca tggtgccttg
    25981 tccatccgcg ccgtgacaga atgcgcaacg cgcggtgaac actttcgcgc cgttcgcagg
    26041 gtcgggaggc ttggtcgatg acaaagtcgg aactccgcgc caggccatgc ggctaccagc
    26101 cggcacatcc ttcgacagcc attcgatgta cgcgacgatc gcttgaagct tgtgactgtc
    26161 ttgcggaatc gccttcccgt tcaaattacg ctcgaaacag tcgttgatac ggtcggccag
    26221 cgtcagaact ttccccgccc gggcccggta ttccggatag gcccgcgaca gccccacgaa
    26281 ggaggccgaa ttgggatcga gtcccgcatc caagtgacaa ttggcgcagg tcagccggtt
    26341 gccgacgtag ggagcagcga attgttgcgt atggacgacg agttcatatc cgaggcgaat
    26401 ctgttccccg cgctgcccac cgggaatgga atcgggcgag ggatggctga agagcgcgtc
    26461 gagcctggca gagtcagatt gactcacggc cgatggtgcg gctccgcgtg tgtccttcga
    26521 ctcgacacag cctgccgtca acccgacgat cacgatgacg ctgaacaaaa catgtcgcat
    26581 gcgtagagag tctcgcaaac tacataccac gtcgacagga ggccgtcaaa aagaattggc
    26641 ttttgacggg catgcgtcgg gatgctccct gataaccttg gcgataaaag ccacagtcct
    26701 cctctgctgc ctgcggcatc gtgctacagc actctggagc ccgcacattc aagatgcagg
    26761 ttagggacag atgtgaggaa aaggattcgg gaaagcatgc atcggcgtca tcctggttcc
    26821 ctcacggctt gagcgaacga atgtacgcca tcacgtcatt caatttttct tcatcgaagg
    26881 ctgcggcata cccggtcatg gcggtgccgg gacgcccttc ctgaatcacc ttgcgcaatt
    26941 gcgcatccgt tttctcttga gtggttttgg aggtgaggtc ggccggtgcc ggcgtgaggt
    27001 aggctgccat ctcgctatcg ccttttccgg caggcccatg gcagttctgg caactctcgt
    27061 gatagagctt tttccccgtg gccggatttc ctttaccgga cgcagccaaa cttatcggca
    27121 tcgatcccag cagcaccccc acagtgcaca gcaacgtgcc ccagattcgc atacccattc
    27181 tctcccttct ggttgtgagg aatttcctca tcaggtggcc cgttttaaac tcagcatata
    27241 cgtggtcaag tcattcaact gctgatcggt gagcgggaat ttcggcataa acgagcccgg
    27301 cgatacggat tgagggtccc ggaaatgctt caagtgccac tcccgatcag gccgcgtgtc
    27361 tccaaccgtc gacaggtccg gcgccaccgc ccctccttcg ccgtgaatcc gatggcaccc
    27421 gacgcaaccg agctgcgcat agacggcacg cccccgcgcc acagccggat ctacccttgg
    27481 taccgcatag agattcctga tcgaaatgcc tagcaggccg aacacgacga gaaggaacag
    27541 caggccgctc ccgagcgcca ccggacgacg gataggattt cgtaccggat tgcggtcgat
    27601 aaacggccag aacagcatga ccaggatcac acagagcggc agaatccagg tcgccaacgg
    27661 ttccagcggc ccgtgcacat acttgagcaa ttcataataa aagagaaagt accattcagg
    27721 caccggcaca aaactcgtat ccgacgggtt cgccttgtcg gtcaggggca gcggaatact
    27781 gaatgccaat gccgcgacaa ccaggaacac gccggcgatg acgacggcgt ccatgtacac
    27841 ctgccgcgga gcaaaggttt cagcacccag tgaggcccga tcatcggtcc agggtccggc
    27901 gggtccgact cgccgcagga cgaagaggtg caccgcaatt cccgtgatga gcatcgcggg
    27961 cagaaacagc acatgaatgg cgaagaatcg tgagagcgtc agcgccccca gaatctcccc
    28021 gccgcgcagg atgcgagagg ctacatcgcc gatcagcgga acggtgccca ccatattgat
    28081 cccgatctgg gtcgcccagt aagccgtttg atcccacggg agaagataac cggtgaaggc
    28141 gaaggtcatc acgatgagga acaggaccac ccctaccatc cacatcactt cacgcggtgc
    28201 tttatacgcg ccatacagaa acgtctggag catgtgaagc ccgatggcca cgaccatcga
    28261 cgatgcgccc caatggtgaa ggcctcgcac caaccagccg agagtcacgt cggtttcgat
    28321 gaagcggaca ctgtcgtagg catggtctgg ggtgggcgcg taatacaacg cgaggaacat
    28381 gcccgtggcg gcctggagaa cgaacaggaa cagcgtcacc gaaccgaaga cataaatcca
    28441 actcgctccg cccggtatcg gctcatcgag caacgtccgc tgaaccggtt tcagattcag
    28501 gcgagcatcg agccaatcgt acaaacgaga aggcattcaa ctcccagttg tgaggcgcga
    28561 ggtaaacctg tcgatggcgc cacttaccag ttgttcatat ctcgacactg gtcggcagac
    28621 ccgacttgaa gtccttgtac atgacgagaa gccgccctcc ttcgactttc gaaggaagcg
    28681 tatcgagtgg ccgcggagct ggccctccca atacctggcc gttcacgtca tacgaactcc
    28741 catgacaggg acagagaaat tttttgtccg tttcgtccca gcggtagccg caccccaaat
    28801 gcgtgcagat cggcgagaac actttgatgt cgccctgcgc ctgcttgacc gcccacaccg
    28861 ccttgtgtga ggtcgtctcc aaccagccgt cccgaatcgt ggtcacatgg tccaattgct
    28921 tggggtgacc ggaagacaag tcatccacag cgccgacatc aacccagtcg cgacggcgcc
    28981 gatgaaaggc cggcgagatc agcgtgctga gcaacggaat ggacagcccg acaccaacca
    29041 gcccggccgc ggctagcgta acccactgaa aaaacgtccg tcgcgaaccg accggcgtgg
    29101 agaatccgcc ttctggctgc tgatcaggct gttctgcatg gctcaccatc gcttgccctc
    29161 gcccgcccca tcttcggcac cgatcaggct ttcttgccag ctccgaccga acaggtgttg
    29221 atgccaaaca ggctccagag cggacagaac cctaaggctc ccgtcaccag cgcaatcgtt
    29281 cccgccgcca aggcgatccc cattcccacc ggcggaagcg ccccaaatac cccgatgccg
    29341 atcaacagaa tgcccagcac aattcggacc ggtcgctcaa ctccacccac attgcatgcc
    29401 atgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaggggc agtttcaaca
    29521 tgatggccgg attgacgtcc gctgccatcg ccatgccggc cgcacgggcg gagctttcag
    29581 ccgccttcaa cggttgatcc cctgctccca gcacggtgac caccccatgg atcgccttga
    29641 gcaagccacg catctccgcc agcaccagat ctcgctcgga tggcgccagg atgatttctg
    29701 tgcggccgtc atcccccggt ctcgtatgac cttgcacgag aaacgtcgac atgatgccga
    29761 tcgtgatcat ccacagtgcc gcaattcccc agccaaagat gcgccaattc atcagatgag
    29821 aggttccatt tctagggacg tccccggcca gcctcactgc ccaattgccg cagataggcc
    29881 gtcacatcac gcatttcttc ttccgatagg acaaacttcc aggcccccat cgccgtgtcc
    29941 cttcgcccat catgaatcgt ttttagtagc gtggcatcca acttggtttg cgcggcagga
    30001 gcggtgaggt cggcgaccgg cggcgtgaag gtcatctcgc cctttccttt tccgtccaca
    30061 ccatggcaac ggatgcacag attggcgtaa agcgtctttc ccttgccgac gttcccgggg
    30121 cccgcataca cggcaaccac aatcgccgac aagaggatca gctgcgagac agcaatcact
    30181 ccgattcgta ggtgcatagt gtgccccttt ttgagcggcc ccatcggaaa gacccaagcg
    30241 ccacacagga gtagatgcga tccccgtgcc aatcgaaccg gcacgaggat ctcggtcaat
    30301 gctctggata gagaccgttt atagaggcgg ggaagaacgc agcacggtgg actggagaga
    30361 atcaccccag cccacacctg atcctggcgc aagatgccac agccggcacg tcagacgatc
    30421 gtgggctcca acgcgatacg ggttttcatc gagttggaga tcaggcaatt ggcttccgcc
    30481 ttttcaatca gttccttcgc tttcgaagca tcccctcctg gcgaaagcgt gatgtgtggg
    30541 cgcagggtga tcaccgtgac ctgaaacttt ccctccacga gttccagacg accttccgct
    30601 tcacagtggt acgcggtgaa cgccaggccg ccccgttccg caacagccag aaatgtggtc
    30661 atgaggcaga tgttcgccgc cgccacatac aaatcctccg gtgaccagat tcctgcatgc
    30721 cccttgaatt caggcggtgt tgccacctga atgtccggct taccttcact tgaaatcgtc
    30781 cctttctttt gttccgtcca ctgaaccgcg gttcgatacg tatacacctt gctgcgtacg
    30841 tccattgata cctccgaact gtgctgggcg catgattgaa aagggtacgg cgacatgcct
    30901 cgtctcgtgc acgacgaaca tccgctcttc ttcagtgaca ctctatggga tgagcaactc
    30961 tgatgccttc accaccacat acctacaggt ggtaatcgcc tgcggcctca ggttggtaca
    31021 gaacgtcctt gatgcgcatg gttcgtcttc ccgccgggac gggccaatcg accgtcgagc
    31081 ccacccggga acccaacaac gccgtaccga tcggcgcgag gatcgatatt ttcctttcag
    31141 cgatgctggc gtccgaagga aacaccagcg tatagacgtt ttccatgccg gtatccacat
    31201 cttcgatccg gactcgtgaa ttcatcgtga ccacattagg cggaatggcg gaggggtcca
    31261 cgacatcagc gcgatcgagc tcctcttcca aactatccag atggtcccga tcacggccga
    31321 cgctcgtgcg ggccctcagc acgtctctga ggcgagacag gtccacggca gtcatgcaga
    31381 tattacgggg attcatgcgt tctctcctct ggtgacatgt cgctccagcg cattcggcac
    31441 cacccggcga tggcctggat cgaattttcc cgtttttacg gtcaacaccg ggcaggtcgc
    31501 ccggcgcaac actgcctctg cgacgcttcc acatcggtcc ggagaaatcc ctctccgccc
    31561 atgcgtcccc atcacaatga ggtcggcatg ttgccgcaag gcggaggcca ggatggcatc
    31621 ggaaggcacg ccgccgcaga gggccacgtc agcttgaagg tcgaaagccg tcaccacgtc
    31681 tcgcaggtcc atcaactgcc gattccaata gtctcgcttc gacgattcct cttcaatcac
    31741 accgagacca cagtccagat catacgaggc cggctccagc acgtgcatca gcgtcacggt
    31801 agcgccgacc cccttggcca ggtgaacagc atattccacc gcatccagcg atggcgtcga
    31861 aaaatccaac ggcgccagaa tatgccgaat cagcgcaggc cgatcggtcg gcggcatttc
    31921 cacggcatcc tgcctcgatc caggcacggc cagcaccgga cagggcccct catgaatcaa
    31981 tcgttccgcc gtcttcccga tcagcccata gagcacattg gtgcgtccct gcgcgcccaa
    32041 caccatcaga tcgatattct gttctctggc ggcctgatga atatgctcgc tgggatttcc
    32101 cagtagcagc ctgactctgg ctggagtgcc tcggcgcgct aactcgtccc gcacctcttc
    32161 caattgtcgc gcagccgcgt cccgcgagcg acccactatg cctagagatt ccgcatccgc
    32221 ctgcatctca tggggcgcct cgatgacatg caagacatcc agcttcgcat cgcaggcgcc
    32281 ggcgagatga accgcatact cttgtgcacg gctggcatcg tctgaaaaat ccgtggcgaa
    32341 taagatgtgc ttcacgaatg gtggtgtcgt catggcttct ccttcacaag ttggacgatt
    32401 gttccgggcg gccggaggtc catcggagag cgccggccga gaactgaacc ggagcgcggg
    32461 actcatgcca tggagagatg aacgtgagga acccaggtgt cgttgacgca agacaagtgc
    32521 cacgtcttgt tcattcacct ccgcccggaa gtccgcggtc gagcctccat ttccggctag
    32581 tgcccctgcc gcgatccaca cgcattgttg acatttgcgg cagccgctac ccaccggtgg
    32641 ggcgatacac cccgctgtgc ccggagcagc gccaatcgat cggccatacg ttcacatggc
    32701 cgccatctgt catccctctg ctgaccatcc atcagtacgg tgcgccatag cgatatccag
    32761 cgtctgcgcg gattgccgct ccgccactgc agctctgagg tgcggcatga ccgaggctgt
    32821 gtcccgtccc agacgcgtga gtgtcttcat gatgcgctcc ggatgattcc aatcgctcca
    32881 ccatactccc tgcatctcca tcactgcggc tacgcctggt acagcagcca ggagatcccg
    32941 tgagaaactt cggaccggca tgcgcgcgta aatgtcgcga agggcttgtg gttccctcgg
    33001 agtattgatc acctcgccca gtcgctcgaa caatctcatc atctccggca ggactctccg
    33061 gccgagattc caccacgtct caacactcgc tgccatgatc cctgtatgcc acagcgctcc
    33121 cgaagcatag gccaaccgcg ccaggtcggg ctccggcctg tgcaagaagg tgttgaccgc
    33181 gcggatcgag cgaaggccca gcctgaccag ttcatgaccg gatttgatcc aggcatactc
    33241 gctgtccggt ccatccggtc gcgcggcaag cccgaccagg ttcgccggca acttttccgc
    33301 cgcccggacg gcgtacaaaa tctccgcggg caaccgctcg tccgggcaga tgaattgatt
    33361 ggccggcgta accaccacca tccccgccgg atcccgcgca cgaatatagg tcaggggcag
    33421 aaacaatccg gctgccgtgc cggcgttgcg gggttgcacc aggaccatgc caggatgtcg
    33481 ctgaccgatc tgcgcccagg cttcttggtg acctcgtccg accaccacga gcgtgcgcgc
    33541 aggactcgtg acgtagcgca tgcggtcgag cgtatgttga aacagagacc tggttccgac
    33601 atatcgacag tagtgattcg gcatcgtccg acccaaccag cgcttcacga ccggctgcat
    33661 gcggactccg tctccccccg caaggatgat cgcccaccgc cgtccgggat gatctccttc
    33721 ttctctcacg acgcctcctc ccggtgtctt actcggataa gtcgagcagg gacaggcccc
    33781 agaagaccat gattcccatt gcgcccatca ccaagaccat gagcaacatc accggcgcca
    33841 aggaaatgac ctgcttcatc gcatcgccct ttcgttctgt catgccgcgc ggcatgacac
    33901 ccatgccatt ccgtcaccac actctaccgg cgcgagatga agtgccgatg agtagggcat
    33961 gaagaattct tcatgctgac gacatgcggc tggaaggaat gacaacgaaa agaggatagg
    34021 cacgactagg aagaacgacg agctgccgga cg
//
LOCUS       DAOQDS010000043        33651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_445_length_33651_cov_19.101798, whole genome shotgun sequence.
ACCESSION   DAOQDS010000043 DAOQDS010000000
VERSION     DAOQDS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 33651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33651
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_445_length_33651_cov_19.101798"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="PK589_11865"
     CDS             <1..1361
                     /locus_tag="PK589_11865"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ20346.1"
                     /translation="MSSERIRTLIIDDEEFVRLVVEQALREEGCDTRIARGGQEGLDA
                     LRTGDFDCVITDLRMPGVDGRAVLRWVKDHQPDVDVIVLTGHGDVKDAVAAIKDGAWD
                     FLIKDTPFDGTAVKAALAKLRTVRELRRENLAARHGGYRQEAMVKGISNAWQTLETQI
                     AQVAPSQAPVLIQGETGSGKEVVARLLHAQSHRAQGPCIAVNCGAVSRELLESELFGY
                     EKGAFTGATTAKPGLIAAAEGGSLFLDEVGEMPGPMQVSLLRFLDRNEYRPVGSTRTL
                     RADVRIICATNRDLQELVFQGRFRDDLLYRINTVTLRVPPLRERPEDIPGLVEHILHN
                     LRLPGTTARTPTPEGLAHLASYRWPGNVRELRNVIERLVLMSPQTGPITGEEVLQVLP
                     RSSTTIVAEDQSQLPLEEIERLHIERVLQACGGNKTKTAHTLQIDYKTLLAKLKKYEA
                     GH"
     gene            1466..2068
                     /locus_tag="PK589_11870"
     CDS             1466..2068
                     /locus_tag="PK589_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNJ20347.1"
                     /translation="MRGPGSSGEQQAQARFGTSDRAAAFYEHQVLASLNQPMREFIAR
                     MEMMHIATADAGGKCDCSFRAGTPGFVQVLDDKTLAYPEYRGNGVLSSVGNILENPHI
                     GLIFLDYYQTTIGLHVNGTARVMDAQEIATLPNITSGMREAAHSKGGRQPKAWIVVGV
                     EEAYIHCSKHVPLLARREKQIVWGTDDERLKGGNFFKITP"
     gene            complement(2110..>4316)
                     /locus_tag="PK589_11875"
     CDS             complement(2110..>4316)
                     /locus_tag="PK589_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNJ20348.1"
                     /translation="LRTLEERLLYLRELEERRQTILASIEEQGKLTDELRRAIEQAAT
                     KQAVEDIYLPFKPKRRTKAQIAREAGLEPLADALLADPTLDPEQEAVKYVKVVAAAEG
                     VEAINVPDAKAALEGARDILVERFAETADLLALLRTKLWNEGIVTSTVLPGKETAEEE
                     KFRDYYAYSETIRTIPSHRALAMFRGRTLGVLKLDLGLGETLDAQVPHPCAALIAAHF
                     GIENKGRRADKWLSDVCYWAWRVKVHLHLSTELLLQVREAAEAEAIKIFGRNLHELLL
                     AAPAGPKAVLGLDPGIRTGCKVAVVDATGKLLDTTTIYPHQPRNDWQGALATLVDLVI
                     RHGVELMSIGNGTASRETDKLAIEVIKVVTERKPEQKVAKIVVSEAGASVYSASAFAA
                     AEFPTLDVSLRGAVSIARRLQDPLAELVKIDPKSIGVGQYQHDVNQRALARSLDATVE
                     DCVNAVGVDVNTASAPLLARVSGLNRVLAQNIVEYRDTHGPFPNRTMIRKVPRLGEKT
                     FEQAAGFLRINNGDNPLDRSAVHPEAYPVVERMLARLNKGIAEVMGKPTVLKDLSPAD
                     FTNETFGLPTVRDILTELEKPGRDPRPEFKTATFRDGVESLADLQPGMVLEGVVTNVA
                     AFGAFVDIGVHQDGLVHVSALANKFVKDPHEVVKPGQIVKVKVLSVDVARQRISLTMR
                     MDDAATAAGQPDLRAGAVREGRDRRQDAPRPASQPQPTSAFALAMARAQQKK"
     assembly_gap    4317..4416
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4417..6465
                     /locus_tag="PK589_11880"
     CDS             <4417..6465
                     /locus_tag="PK589_11880"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HNJ20349.1"
                     /translation="CDDRSEFSRRFEELPGRNDASYVLTHRHSCVGHGIVAFATGSRL
                     HDGPSAPDVPHMQKHLQTSSASASLTVAAMGVVYGDIGTSPLYALRECFHESHGLAVT
                     PENVLGILSLIVWSLVLVVTVKYLLFVMKADNDGEGGMLALMALSQRSHPVNLRKGLS
                     LVVSLGLVGVAFLYSDGIITPAISVLSAVEGLTLATDLFTPYVLPITVGLVALLFTIQ
                     RRGSGALGSVFGPIMLVWFAALALLGLHSLIQTPAVLLAANPLYAATFLLRHAGIGFL
                     VLGSVFLVLTGAEALYADMGHFGRRPIQLGWFAVALPALLLQYFGQGALLMRNPAALA
                     NPFYHLAPDWMLYPLIALATVATIIASQAMLSGAFSLTLQAVQLGYLPPLRITHTSAE
                     QHGQIYFALLNWLMFIGTVGLILSFGSSTNLAAAYGIAVSGSMIITTLLMYRVTRSHL
                     GWSALPALMAVGTFLCIDLAFFLANAQKIPHGGWFPLLLGAAIFTVMSTWARGRAIVA
                     EHLRVQFPPLHQFVGDVLSKVEYRTAGQAVFLSQHPDITPPALLQNVRHNKTLHEHVY
                     LLTVRTEHVPFVPAGAPLEITPVYEGLFQVIARCGFMETPDIPRVLTQLAANGHVLPL
                     QNTTFFLSRITFLATPKPGMAIWREKLFVVLSRNTQRASSYFHLPSEQVVEIGLVLEI
                     "
     gene            complement(6536..7000)
                     /gene="trmL"
                     /locus_tag="PK589_11885"
     CDS             complement(6536..7000)
                     /gene="trmL"
                     /locus_tag="PK589_11885"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HNJ20350.1"
                     /translation="MFDVILYQPEIPPNTGNIIRLCANTGARLHLVKPLGFTLEDKQL
                     LRAGLDYHEFATLAVHETWETCLQRFTGQRLFAVSTKGRQRYDLVQYAEGDAFVFGPE
                     SRGLPAEILASVSDQQRIRVPMVPGSRSLNLSNSVAVVLYEAWRQAEFGQCL"
     gene            complement(7098..7556)
                     /locus_tag="PK589_11890"
     CDS             complement(7098..7556)
                     /locus_tag="PK589_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNJ20351.1"
                     /translation="MMNEQDTQRAVAILNRIMELELAGVVRYTHYALMIYGYNRIPIV
                     SWLKGNADESLAHAHKAGELVTLLGGHPSLKIGTLLETEKHDIGDILRESLEHEKAAV
                     ASYYELLKLAEGKSVLLEEFAREMIVGEELHLDEVNKMLRKSGDVQAFRS"
     gene            complement(7611..8702)
                     /gene="ychF"
                     /locus_tag="PK589_11895"
     CDS             complement(7611..8702)
                     /gene="ychF"
                     /locus_tag="PK589_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNJ20352.1"
                     /translation="MSVKCGIVGLPNVGKSTLFNALTKSGIAAENYPFCTIEPNIGIV
                     EVPDARMQALADIVKPQRMQYATTEFVDIAGLVAGASKGEGLGNQFLANIRETDGIVN
                     VVRCFEDDNVVHVAGKVDPISDIATIVTELALADLTTVEKAQERNVKLIRSGDKDAAK
                     LGDLLVQVAACLNEGKPARTMKLDPAQRALLKPLCLLTMKPVMYVANVSEKGFANNPL
                     LTRVEDYAAQEGAPVVAICAALESEIAVLSDEEKQEFLADIGMKEPGLNRLIRAAYQL
                     LGLQTYFTAGVKEVRAWTIHIGDTAPQAAGVIHGDFEKGFIRAEVIGYDDYIACKGEA
                     GAKEKGKMRLEGKEYVVRDGDVMHFRFNV"
     gene            complement(9019..10080)
                     /locus_tag="PK589_11900"
     CDS             complement(9019..10080)
                     /locus_tag="PK589_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNJ20353.1"
                     /translation="MGRSGMLLCAMVVMWAGLWEVGAAAEPSSVPAAEALRIMQFVLT
                     GTVSTERARGFVNEAQAAGFTAVQVLLTDGVQFDHAPWIPVKTAWTKAEFLSWADYAR
                     AHGLEVIPEVKLLTHQEQFFQRQYPGLMFNTVSYDPRKDATYAVVFPFLDELIEAVHP
                     RAIHIGHDEAFGWTVGQVSKWLKLGEVMIPADLFLRDVVRVHDYLGKKGVETWMWADM
                     VLSPAEFPGAQTRHLHGIAAGYGKALRQQLPRDIVMCDWHYGDEQGSFPSIDIMQGEG
                     FRVIGATWKQESATRKFSRYALSRHAYGLMATTGFHIQQNDTKMIHWIIQASGALFRN
                     PEAVVPPMPASAGSSEGRG"
     gene            10590..11258
                     /locus_tag="PK589_11905"
     CDS             10590..11258
                     /locus_tag="PK589_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20354.1"
                     /translation="MDRSQAGTVRSMSFACVGLTLVSLLTGALPAAALESSARTIVSQ
                     SNSHVGLSIDLAGFTIERDQLKPNGRRYLMASHPGTGIKVAIALEQVAGKASTAGCIE
                     HLQHVQKGSAVSRGKDVTLSTAHEMPTLEYTLPRFQGVRLDQKSLYACMAEGNVYANI
                     HVSKVRYTDADASRFRQLLTTLRLQSGPSPRMTAHQSNSAAPLFSVDTVRYEQKNEAR
                     TIAP"
     gene            complement(11271..12488)
                     /locus_tag="PK589_11910"
     CDS             complement(11271..12488)
                     /locus_tag="PK589_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20355.1"
                     /translation="MMNRLTRDRAQTDLLTHRHRSVVWVLGAGALLSAFLFVPAAQAN
                     HRDHQPHADAEVPGVLGQGREIPGAPFKVFLSTGAVADVSGLDDADLDAAVQTVIDTF
                     IIMLQHRADYPRFEESLTKQALKQVVIEPVVVNDEGKAFPFLVARTKEPGRVTLLISA
                     TSLKDRGYLNHPDSLLPVLSREFQWVVSKADTAPKAKAVAVERNLEKAPILTDEEIAG
                     LSAEERVRLLQQLFDTYLRTVDDQRSLDGQPWYEVGHSAPLPPTHADSTTKLYDIRIR
                     EALQKIVRDPFFLERTPKAVRNLLNGKVWNVAYVKIDQRDWSTRTRVLSEEKSVVVGK
                     RERRIQPAAILVNVHRLAAADDPFYRETQGLPMGALSADQLAHVIALEIHHNIQEKSL
                     SGHTAQDALTAPR"
     gene            complement(12500..13525)
                     /gene="galE"
                     /locus_tag="PK589_11915"
     CDS             complement(12500..13525)
                     /gene="galE"
                     /locus_tag="PK589_11915"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNJ20356.1"
                     /translation="MRMILVTGGAGYIGSHTCVELLNAGSAVTVFDNFSNSHPEALAR
                     VQRITGKSLRLIRGDIRDRAALVAALRESGATSVIHFAGLKAVGESVEKPLSYYDNNV
                     VGSLRLLEAMGECGVTTLVFSSSATVYGDPQRLPLTEDHPLSATNPYGRTKLMVEEIL
                     RDLQRSHHSWRIGILRYFNPVGAHASGLIGEDPQGMPNNLLPFVAQVAVGRRDHLNVW
                     GNDYSTPDGTGVRDYIHVVDLALGHLKALKALDGWDRPKECLTVNLGTGNGYSVLEIV
                     RAFEKASGKAVPYKIAQRRPGDVASCYADPGYALKALGWQAARGLDDMCTDAWCWQYN
                     NPNGYTG"
     gene            13688..14548
                     /locus_tag="PK589_11920"
     CDS             13688..14548
                     /locus_tag="PK589_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNJ20357.1"
                     /translation="MQHQHNTNYYAILELSADATEADIKRAWHEQMQIWHPDRFLHSS
                     TLHKKAEARTQLINQAYQTLSDPTARARYDSGRQQPSAPPPPPRPSSPPRPQPVTRPR
                     QELRGPQTMLNVTRFSHPKIMVPAIHLLVDVHETQPYAFKGLIRIAGTRTQALPAGDY
                     AIAEAPGIFCVERRRAEEFNTIVSNPSDNRPRFLRELEPLLAIPHRFLVIEGALHSHR
                     GGGRLEQYHRNGLIDFLDSITARFGLQIIYADSRDEAEERVANLAAIHYAYYLAEQQG
                     LGRCLTENDA"
     gene            complement(14650..15660)
                     /locus_tag="PK589_11925"
     CDS             complement(14650..15660)
                     /locus_tag="PK589_11925"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNJ20358.1"
                     /translation="MTPDLENSCKDEEARATPVATFWRRTGAAVADVLLLGAIGACLG
                     FLWFDQLAALGRTGRFIGAAIALVYFATLNSQIGQGQTLGKRLAKIRVTDAKGALISL
                     PRSAFRATIFFAPLALNGTATPPGPYEQLVGIVLSILIFGVGVAQAYLYCCNRRTGQA
                     LHDIVVGTFVREAESASEIQQKMWKPHLAIAGGLSTLVLGVLLLPYTSDPPDFVSQLA
                     EARRLAQSVVPDAHVSISRQTSMMTTPGSGQTPASRVQLSVEVRVKPENFESVADNIA
                     MAMFRSSSGVGDVGQVVIVISYGYDIIISHARTTKVFAHTPQEWLKRLKLDELHNVVP
                     PA"
     gene            15870..17432
                     /locus_tag="PK589_11930"
     CDS             15870..17432
                     /locus_tag="PK589_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ20359.1"
                     /translation="MPLSTTVHDLRTLIRSSHPLIVIETVEEDRVMTLLQSVAAQERM
                     PLFEWSITRGLTRPEEGPSLSKLTATPLAALQHLNGLTVEGIFWLKDLAPHLQDAAVA
                     RQLREVSHHLSRSRATCVMTGHPITLPLDLDQIAVRFDLQLPDRDELHAMLQSVLQSL
                     RTRTSPRRPTSTTVVQSLLNSMATPKPTMAGPSDQDCEAILRALQGLTLHQARQVITQ
                     CVIEDGVLSADDVQTILTRKVQAIRDGGLLEYYPLEDNRFELGGFVKLKSWLERAKMG
                     FTAEAKALNLTPPRGIMLVGVPGCGKSLAAKAIAREWHLPLLKLDAGRLFDKFVGESE
                     KNFRKAIEMAESLSPIVLWIDEIEKAMSAGGGSGDADGGLSRRLFGAFLTWLQEKKQD
                     VFVVATANNLSALPPELLRKGRFDEIFFVDLPDKDERTAIWSIHLNLRKQDRAQFDLP
                     KLVDASKGFSGSEIEQAVVAGLYRALYHKQPLTTDLLIQELSHTVPLSVTRSEEINQL
                     RATAQGRFVNVR"
     gene            17687..19432
                     /locus_tag="PK589_11935"
     CDS             17687..19432
                     /locus_tag="PK589_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ20360.1"
                     /translation="MAVPKDRQVRHHGRLLSLSVMLLLTPIAGTGSGAFANDLPLRSE
                     GQIAQHAKAAWEQGTLNLALGIVEQGIQDHPRALALHQLRGDMLATSRQTEAALESYE
                     TVLASIPAALDVRWAKWSVLVRSGQVEEAVAELQRIAVIDPHNPLLHLRLARELRKLD
                     RLEESFEAYQKAVELGPDMLNWRLALARARFDVLDYEGAERDVQFVLDRVPPGSPLHL
                     SARNLLTVFFGSTERGRRFAPVMTPDVSPKQLRDWSMIRAEGYRLFEAGRFREAEPVY
                     RELLRLNPMDPLAGRHLSLILMGLGRCKDALAVSQAGSEFDPTDEEHTTTAFRMGQCM
                     VELGRWEDAYMQFKTLYDATVEFEKSTKDVQLPSGTRVLNKANLVQWLEKVRLHVPEF
                     AQQVDEDAAATAAQAKAAAPTIPPEAELAAKAIEALKPQNTLDPAASLVGRDADFAWF
                     RFVIPAKKVMRDDSPTGAHDFIPLDPGISFPGSQQDIYLVFSLVTASYDEVPLAARCF
                     KETAELAGAHTAVAQDRLIMSTNDQSGYFVLPRPATGWTAGLYRCGLFAGEETSAYTQ
                     VDEVRFRIIEPTRPS"
     gene            complement(19503..20456)
                     /locus_tag="PK589_11940"
     CDS             complement(19503..20456)
                     /locus_tag="PK589_11940"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20361.1"
                     /translation="MMSAPTPDTETTIAFENQRAVLSPWGASLRRYFIVGSSGSETDI
                     VWGYSGGGQKKGGQGDVLIPFPGRVAGGRYSFDGQLLQLERNDKEGPNAIHGFVRSLP
                     WTTHQAVAHAVTYEVSVDAADYTSRGYPFSLVVRLTYSLGIQGLSCTFSVRNVGGAAA
                     PVGVGFHPYFTVGTGLIDEAEAKIPAAGFLEFNEQLAPTGKVIAATGTEWDYRTFQRI
                     GPRRFNHCYLELERDAAGMATASLRNHKSGRTIDVVMDRAFSAVVVYTGDAIADAPRR
                     ALAIEPMTCATDAFNHPDWGLKRLLPGEVFTGSYSVRARSR"
     gene            complement(20472..21737)
                     /gene="pfp"
                     /locus_tag="PK589_11945"
     CDS             complement(20472..21737)
                     /gene="pfp"
                     /locus_tag="PK589_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNJ20362.1"
                     /translation="MAIREQRPVVGILVGGGPAPGINSVIGAATIRSILGGSDVIGIQ
                     DGFKWIMDGNTSKVRRLSIEDISRIHFSGGSCLGTARANPTQKTEHLDACLASLHTLG
                     VTRLITIGGDDTAFSALKLEQRAGGRLQVVHVPKTIDNDLDLPHGIPTFGFQTARHIG
                     VELVKSLMVDAETTSRWYFVVTMGRKAGHLALGIGKAAGATLTIIPEEFRHKPIRLQT
                     LVDILVAAFIKRLSAGRSDGVAVLAEGLVEILDQQDLNGLEDVERDQHGHVRLAEINF
                     GFVLKRAVEKELRTFGIKTTIVDKNIGYELRCADPISFDMEYTRDLGYCAAQFLLEGG
                     NAAMVSIQNGRFIPIPFGDILDPATGRTRVRMVDVESESYVIARRYMIRLSSDDLHQP
                     DDLVRLATTAGLASDDFRKRFEYLIGKDV"
     gene            21967..22347
                     /locus_tag="PK589_11950"
     CDS             21967..22347
                     /locus_tag="PK589_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ20363.1"
                     /translation="MVEILVVDDNLQLRDTLRKILNKRPGFRVTGEAADGIAASQLAQ
                     TLRPTVVLMDVGMARMDGVEATRRIRALLPNTVVIGMSCHNSREVEVALRAVGANAFI
                     PKELVPEQIFDVISQQLPQDQTVQ"
     gene            complement(22380..25607)
                     /locus_tag="PK589_11955"
     CDS             complement(22380..25607)
                     /locus_tag="PK589_11955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ20364.1"
                     /translation="MASDPPPSASSSQDGIQQGGHSWWARLAEWNPSLLTTVTLAVAT
                     GLRMALTPLWGSGYTFITYYPAIMFIAVANGWRHGVAATLVSSVLTIVLFFDMQASPG
                     EQQTALALFITANVIIVSLSEAVTRARLRAQAETSLVRAQEQRLRLEIDARAKAEETV
                     RASERQMQFVTDHAPVLIAQCGTDGRYRFVNQRYADLVRRTPEDLIGLHPREVLGAEA
                     YQQAEPYMREALAGRQVKFDQQFLGAASIGRLLQVTYAPEWDERGIVTGFIAAIVDIT
                     ERKQAEQARATLAAIVESSEDAIVSKDLNGLITSWNRGAERLYGYTRQEAVGQSVLLV
                     IPDEQIEEETPILAKIVRGETVQQYETIRRRKDGTLIDIALTVSAIRNDEGRIVGVSK
                     VAHDITERKRAEAALRESEERLRLFIEHAPAAIAMFDRQMRYVAVSRRWLADYGLEGE
                     VLGRSHYEIFPDIPERWRRAHQEGLAGVVMRIEEDRFERSDGTVYWLRWEVRPWRDKA
                     GGIGGIIIFAEDITDSKKAEDALRESEAQHRATFDNAAVGIAHVGLDGRWLRCNDALC
                     ALTGYSREELLARTFTEITHPDDIEGDWASVRRLLAGEIETFSMEKRYIRKDGSLIWG
                     HLTVSLLRDEQGSAHHFISIIQDIQIRKEAQAQLRQLADDLEQRVEVRTRELADTQDK
                     LRALTTELNLTEHRIRKQLATDLHDYLAQLLVLSRIKLGQAKLEEVTPAAMEAMTEVQ
                     EVMDRALAYTRTLVAQLSPPILNEFGLLMALRWLADQMQRELTVKLDLESDVLSLSEE
                     QSVLLFQSVRELLMNIVKHAGTTEAKISVTQADGVLRICVSDRGVGCDVGPGEAKLEP
                     GFGLFSIRERMKALGGEFDMQSVPGEGTTAILQLPVVQPGPTADEAVAETGVAAVSHA
                     VVSEPAAPGVPKLPTTASLLATPKIRVLLVDDHRMLRQGLRTIVNGHPGLEVVGEARD
                     GLEAIELTRTLSPAVVVMDVNMPRCDGVVATKRIKEEYPEIKIVALSMHNSPDIVARM
                     KQAGAYGYLTKESAGVQLCRAIVEAALSRPSGASGEGRDVSCGPS"
     gene            complement(25799..26179)
                     /locus_tag="PK589_11960"
     CDS             complement(25799..26179)
                     /locus_tag="PK589_11960"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ20365.1"
                     /translation="MAPRRYARTYYRFPLNYPVIFGGAPFVGEGVLTNLSLKGCSVTC
                     DREVLCGSQVRVSMLLHHQPPALPVELGTIKWVRGNQFGVEFVRVPLEAQQRLNRTLR
                     MELIEWLENRHRNSASPASPRHDN"
     gene            26359..26880
                     /locus_tag="PK589_11965"
     CDS             26359..26880
                     /locus_tag="PK589_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20366.1"
                     /translation="MHKSLRHAAWIIAIAMLPGCVGLGAWTLGTRSESSDHPKIDQTR
                     GAIDLRKAGTPSEFKTVAELRTQWGEPDNIELRKDGREEWIYKTGGLRWSGMVLYLVV
                     IPLPAMIPVGSQYVSLVIHDGRIEQATRRDWAFKAGAYCGFFGMVYGGLGCGTGTFEE
                     KQGSDAQVVNGTP"
     gene            complement(26956..27459)
                     /locus_tag="PK589_11970"
     CDS             complement(26956..27459)
                     /locus_tag="PK589_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20367.1"
                     /translation="MSKFLIQAIVVLALVGPGGQFVRADEGSSKSHHQHLQGVVIEKG
                     GALAVKVPNGSTYQLNENRSERHGHALPKAGDEVTVVIDENNNVLEVHPKGTEGKHRF
                     ITGEVVSIGVMQREITLKTSKGEQKFAMEKQELSSSIKDGARVRAELNEAGSIIDVHP
                     EKSGGGH"
     gene            complement(27676..28428)
                     /locus_tag="PK589_11975"
     CDS             complement(27676..28428)
                     /locus_tag="PK589_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20368.1"
                     /translation="MGATEELAKKFQTPLTDVVSVPFQYNTFFGVGPGNATSHLLNIS
                     PVVPVRLGDWHVVTRTVIPVISVSRFTPGLEDEIPGTGGGTATGLGDINFTPFLTPGR
                     TGAVSWGLGPSITLPTSTMEELGSKKWSAGPSAVVMFRFSPVVGGVLLRQQWSFAGGR
                     HHPGVSQFLMQPFASYNLSGGWFLTSAPIMTANWEAQPGNKWLVPVGGGVGRAFILDH
                     QPMSLTAQAYYHVIRPDLGAEWQTRLTMQFLF"
     gene            complement(28819..30072)
                     /locus_tag="PK589_11980"
     CDS             complement(28819..30072)
                     /locus_tag="PK589_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ20369.1"
                     /translation="MSDTHRWLLTRDFGFMWWSQVLSQVAEGVSKLALLWFVYAVTGS
                     PLKTTVIGLLQTLPPILFGPFIGVLVDRFPKKAILITSDVARGLLIGLIPCLVPIEHF
                     TVESLYVLTFLFGMATAVFVPTLSSAVPFMVARPQFTAANALLQSTTSLGIIIGPAVS
                     GLGIAFSGSQDVLCVNALTYFASAACLLPIRLRATEQAPDSSAGWRPFIRHLLEGLRY
                     AFLTHRTLLILIVLASIYTFGSGAFTTLFPVFGRQLLGLGPVEVGYLWSWLGVGLLLS
                     SIGLIWLSAWDLHKRLLAIAVSCALAGLAVVGLTWTDSVGVASAYVVCIGIGLGTWTP
                     IAWGIIQEVAPPGMVGRVMAVYTAMATATSMLGMSAFGWVAESFGSITSVLGIGGVMG
                     VLAWGSAWFGGRIRTVGVTEGGNRS"
     gene            complement(30233..31495)
                     /locus_tag="PK589_11985"
     CDS             complement(30233..31495)
                     /locus_tag="PK589_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ20370.1"
                     /translation="MSDDHSTPDSSRGWRLICTRDFGLLWWGQTTSQIGEGLNKVALL
                     WFVYELTGSAMKMTMVGLLQTIPPLVLGPLVGVYLDRMPKKPVMIWVDILRALLTLLI
                     PTLYALDMLSITGLYGIIFLTSVVSTIFGPALVSAVPLLVRPSELMSANALIQGTNNI
                     GMLLGPAISGIMIALIRAENVLFVNSATFLISALCLMPIRFSPSHAVSSEQATSVWQE
                     LQVGFRFIFGQQSMVLTLVIISSLYNLGVSAFVFILPVYAKEFLQVGPVQLGWLWSAL
                     GVGMLAASAWLALKQESDIQGRLRIVVSGMTIGGLAVCSLSLLETPLIAAGIVIIVGG
                     STAVLNPIIWALLQELTPEHLIGRVVTTFSVGSMASAMAGMTGFGWVADAVGPAASLV
                     ALGLVLLLTAAVAVACVRRTCALPVAHA"
     gene            31849..32283
                     /locus_tag="PK589_11990"
     CDS             31849..32283
                     /locus_tag="PK589_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ20371.1"
                     /translation="MPSSCSILLIDDSPGECELFRQALTQTGYKGRLDIVEGGQAAMA
                     YLHEHVQHEEPALILLDLKLRGERGVDVLKRIKQEARVAHIPVVILTSSDDTADVRAC
                     YSGGANGYVVKPGQFDDLVTLSLHLWKFWLEHNCTQRMAAPC"
     gene            32277..>33651
                     /locus_tag="PK589_11995"
     CDS             32277..>33651
                     /locus_tag="PK589_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ20372.1"
                     /translation="MLTRAALKNPYAVFALCMIALILGGVSYQKMRVDIFPEIKLPSI
                     LVTTFYRGLSPSEMEGAITLKMEQRFVEASYVEHIESQSLAGMSYIKVFFQPDYGIDA
                     AQSELTSLAYSIIRLLPPGVYPPSVYKFGVSSLPVGYLSISSDALGPKEIRDLAYFTV
                     RQQIANIPGISFGPPLGGKVRQITVFLDQQRLLARGISPSDVVNALNQQSAIIPAGDI
                     KIGDLDYYVYSNSLVDVVDKINDLPIKIVNGTPVLIRDIGTAADSAAVQTSIVRVNGR
                     EATYIPITRQEGANTLEVTDGIRAKLSKLTEIPAGTTVKFLYDQSLYIRQSIANLQKE
                     GLLGAGLAGLMIFIFLASVKAALVVGLAIPLSLTVALVALYLTGQSVNIMTLGGLALV
                     IGTLLDNNIVVQENLHRHLEMGKDGRSAAEDSAMELTLPILVATICILIVYLPIMFFT
                     GIIKYLFV"
BASE COUNT         7074 a        10431 c         9291 g         6755 t
ORIGIN      
        1 tcatgagcag cgaacgcatc cgcaccctga ttatcgatga cgaagaattt gtccgcctgg
       61 tcgttgagca ggcgctccgt gaggaaggat gcgacacgcg gatcgcacgt ggcgggcagg
      121 aagggctcga tgcgcttcgc accggcgact tcgattgtgt catcaccgat ttgcgaatgc
      181 ccggcgtcga cgggcgcgcc gtgctgcgct gggtgaaaga ccatcaaccg gatgtggacg
      241 tgatcgtcct gaccggtcat ggtgacgtca aagacgccgt ggcagccatc aaggatgggg
      301 cctgggattt cctgatcaaa gacacgccat tcgatggcac ggccgtaaag gccgccctgg
      361 ccaaactgcg aaccgttcgc gaactccgcc gcgagaatct ggcagcgcgg catggcggct
      421 atcgccaaga agccatggtc aaagggatca gcaacgcctg gcagacgctg gagacccaga
      481 tcgcgcaagt ggcaccctcg caagcgcccg tcctcatcca gggtgaaacg ggttcgggga
      541 aagaagtggt ggcgcgcctc cttcacgcgc agagccaccg ggcccaaggc ccgtgcattg
      601 ccgtcaattg cggggctgtg agccgcgagt tgctggaaag tgaattgttc ggctacgaaa
      661 aaggcgcgtt caccggcgcc accacggcca aacctggcct catcgccgca gccgaaggcg
      721 gctcgttgtt tctggatgaa gtgggagaaa tgccgggccc tatgcaggtg agcctgctgc
      781 gctttctcga ccggaacgaa tatcgtcctg tcggcagtac gcgaacactc cgggctgatg
      841 tccgcatcat ctgcgccacc aatcgggatc ttcaggaact cgtctttcag ggacggtttc
      901 gcgatgacct gctctatcgc atcaataccg tgaccctgcg cgtgccgccg ctacgggaac
      961 gccccgaaga catccccggc ctggtcgagc atattctgca caatcttcgc cttccgggaa
     1021 cgaccgccag aacaccgact cccgaagggc tggcgcattt ggcctcgtat cgttggcccg
     1081 gtaatgtgcg cgaactacgc aatgtcatcg agcgcctggt cctcatgagc ccgcagaccg
     1141 gccccatcac cggcgaagaa gtcctgcagg tccttcctcg atcatccacg accattgtcg
     1201 cggaagacca gtcgcagctt cctctcgagg aaatcgaacg gctgcacatt gaacgcgtcc
     1261 tgcaggcctg cggcgggaac aagaccaaaa ccgcccatac gcttcagatc gactacaaga
     1321 ccctgttggc caagctgaag aaatacgagg ccggccacta gcgtaggcag tctggcgtga
     1381 gtcctggcat cgactgcaag gacggactgc ctgacgatat cggtgccttg attcgttcga
     1441 aacaggaggc aaaggagcag caagtatgag agggcctgga tcatcgggcg aacagcaggc
     1501 acaggcacga ttcggcacgt ctgatcgcgc cgcggcgttc tatgaacatc aagtcctggc
     1561 gtccctcaat caaccgatgc gggaattcat cgcccgcatg gagatgatgc atatcgcgac
     1621 cgcagacgcg ggagggaaat gcgactgttc cttccgcgcc ggcacaccgg gttttgtgca
     1681 agtgctggat gacaagacgc tggcctatcc ggaatacaga gggaatgggg tgctgtccag
     1741 cgtggggaac atcctcgaga atcctcacat cggcctgatc tttctggatt actatcagac
     1801 gactatcggg ctacacgtga atggaacagc gcgcgtcatg gatgcacagg agatcgcgac
     1861 gttacccaat atcacttccg gcatgagaga agcggctcac agcaaggggg gccgtcagcc
     1921 caaggcctgg attgttgtcg gcgtggaaga agcgtacatc cactgctcca aacatgtccc
     1981 gcttctcgca agacgcgaaa aacagatcgt ctggggcacg gacgacgaac ggctcaaagg
     2041 cggcaacttc ttcaagatca cgccctaggt ctactcccgg tcacagatag ctgcgtcgaa
     2101 cacacacact cattttttct gctgggcgcg cgccatggcc agagcaaaag cgctggtcgg
     2161 ttgaggctgg gaagcaggcc gcggcgcatc ctggcgccga tccctcccct cgcgtaccgc
     2221 ccctgcgcga agatccggtt gtccggcagc cgtcgcggca tcatccatac gcatggtcaa
     2281 cgaaatgcgc tgacgagcca catcgacgga taacaccttc actttcacaa tctggcccgg
     2341 cttcacgacc tcgtgtggat ccttcacaaa cttgttcgcc agggcggaca catgcacgag
     2401 accgtcctgg tgcacgccga tatcgacgaa ggcgccgaac gccgcgacat tggtcaccac
     2461 gccttcgagc accatcccgg gctgcaaatc agcgagtgac tccacgccat cgcgaaacgt
     2521 cgccgtcttg aattcaggac gcgggtcgcg gccgggcttt tccaattccg tcagaatgtc
     2581 ccggaccgtg ggtaagccga atgtctcgtt ggtgaaatct gccggcgaga gatctttcaa
     2641 cacggtgggc ttccccatga cctccgcgat tcctttgttc aaacgcgcca gcatgcgctc
     2701 gacgaccgga taggcctccg gatggacggc ggaacgatcc aagggattgt cgccgttatt
     2761 gatccgcaaa aatcctgccg cctgctcgaa ggtcttctcg cccaagcgcg gaaccttgcg
     2821 aatcatcgtg cgattgggga acggaccgtg cgtatctcga tactcgacga tgttctgcgc
     2881 caggacccga ttcaagccgg atactctcgc caggagtgga gccgaagccg tgttcacatc
     2941 cactccgacc gcgttgacgc agtcttcgac cgtcgcatca agcgaccgcg cgagcgcccg
     3001 ctgattgacg tcatgttgat actgccccac cccgatggat ttgggatcga tcttcaccaa
     3061 ctcggccagc ggatcctgca gccgtctggc gatggacacg gcgccacgta aactgacatc
     3121 gagcgtggga aattccgcgg ccgcgaaggc cgaggccgaa tagacggacg ctcccgcctc
     3181 actcaccacg attttggcca ccttctgttc cggctttcgc tccgttacga ccttgatcac
     3241 ttcaatagcc agcttgtccg tctcacgact ggccgtgcca ttgccgatcg acatcaattc
     3301 gactccatgc cggatcacca agtctacgag ggtcgccagc gccccctgcc agtcattgcg
     3361 cggttgatgc ggatagatcg tggtggtgtc caggagttta cccgtggcat ccaccacggc
     3421 cactttgcac ccggtacgaa taccggggtc gagcccgagc accgctttcg gaccggccgg
     3481 cgcggcaagc aggagctcgt ggagattgcg gccgaagatc ttgatcgcct ccgcttccgc
     3541 cgcctcccgc acctggagca gcaactcggt gctgagatgc agatgtactt tcacccgcca
     3601 cgcccagtaa cagacatcgc tgagccattt atcagcgcgc cggcccttgt tctcaatgcc
     3661 gaagtgggct gcaatgagcg cggcgcaggg atgcggcacc tgagcatcca gcgtctcacc
     3721 caggccaagg tccagtttca gcacaccaag cgtccggccg cggaacatgg ccaaggcccg
     3781 atgcgaagga atcgtgcgaa tggtttcaga ataggcatag taatcgcgga acttttcctc
     3841 ttcggccgtc tctttgcccg gcagcaccgt cgaggtcaca atgccttcat tccacaattt
     3901 cgtacgcagg agggcgagga gatcggcggt ttcggcgaat cgttcgacga ggatatcgcg
     3961 agccccttcc aatgccgcct tcgcatcagg cacgttgatc gcctcgacac cttcggctgc
     4021 ggccaccacc ttcacatact tgacggcttc ctgttctgga tcgagggtcg gatcagccag
     4081 cagcgcgtct gccagcggct ccagccccgc ctcgcgtgca atctgcgcct tggttcgacg
     4141 tttgggcttg aacgggagat agatgtcttc gaccgcctgt ttggtcgcgg cctgttcgat
     4201 ggcgcgccgc agttcatcag tcagtttgcc ctgctcttcg atcgacgcca ggatcgtctg
     4261 tcgccgctct tccaattcac gcagatacag cagccgttct tccaatgtcc ggagatnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgcg atgaccggtc ggaatttagc
     4441 agaagattcg aggaacttcc aggccggaac gatgccagct acgtgctgac gcaccggcat
     4501 tcatgtgtcg gacatggtat agtcgcgttc gccaccggaa gccgcctcca tgacgggcct
     4561 tcggcacccg atgtcccgca tatgcagaaa cacctgcaga cttcatccgc atccgcatcc
     4621 ctcaccgttg ccgccatggg cgtggtctat ggagacatcg gcaccagccc cctgtatgcc
     4681 ctgcgtgaat gctttcatga atcgcatgga ctggcggtca cgccggagaa cgtgctgggc
     4741 atcctctcgt tgatcgtgtg gtcgctcgtg ctcgtggtca cggtcaagta tctgctgttc
     4801 gtcatgaagg ccgataacga cggcgaaggg ggcatgctgg cgctcatggc actcagtcaa
     4861 cgctcccatc cagtcaatct gcgcaagggc ctaagcctcg tcgtctcact ggggttggta
     4921 ggcgtcgcct ttctgtacag tgatggcatc atcacgccgg ccatttccgt cctcagcgcg
     4981 gtggaggggc tgacgctggc caccgatttg ttcactccct atgtgcttcc gattaccgtc
     5041 ggcctggtcg ccctgctctt caccatccag cgccgcggct ccggagcgct gggaagcgtg
     5101 ttcggtccca tcatgctcgt gtggttcgcc gccttggcct tgctcgggct gcacagtttg
     5161 attcaaacgc cggcggtgct gcttgcggcc aatccgcttt atgccgccac gtttcttctc
     5221 cgccatgccg gcatcggctt tctcgtgttg ggtagtgtct ttctggttct cacgggagct
     5281 gaggccctgt acgcagacat ggggcatttc ggacgtcggc cgatacaact cggctggttt
     5341 gcggtcgcgt tgccggcgct gctgttgcag tatttcggcc agggtgcgct cttgatgcgg
     5401 aatccggcgg cgctcgccaa tcccttttat cacctggctc cggactggat gctctatccg
     5461 ctgattgcgc tggcgacagt cgcgacgatc atcgcatcgc aggccatgct gtccggggct
     5521 ttttcactga cgttgcaggc ggtgcaactc ggctaccttc cgccgctccg gattacgcac
     5581 acctccgcgg agcagcatgg ccaaatctat ttcgccctcc tgaattggct gatgttcatc
     5641 ggcaccgtcg ggctgattct ctcattcgga tcgtcgacca acctggccgc ggcttacggc
     5701 atcgccgtat ccggatccat gatcatcacc acgctactga tgtaccgtgt cactcgcagt
     5761 catttgggat ggagcgcact gccagcactc atggccgtag ggacatttct atgtatcgat
     5821 ctcgcttttt ttctcgcgaa cgcgcagaag attcctcatg gtggatggtt tccactgctc
     5881 ctcggcgcgg cgatttttac cgtgatgagc acctgggcga ggggtcgtgc gatcgttgcc
     5941 gagcatctcc gtgttcagtt cccgcccttg catcaattcg tcggagacgt cttgtcgaag
     6001 gtagagtatc gaaccgccgg acaagccgtg ttcctttcac aacatcccga catcaccccg
     6061 ccggcactgc tgcaaaatgt gcggcataac aaaaccttgc acgagcatgt gtatttgctc
     6121 accgttcgga cggaacatgt gccgtttgtt ccggcaggag cgccattgga gatcaccccc
     6181 gtgtacgagg gtctttttca agtcatcgcc cgttgcggct tcatggagac gccggatatt
     6241 ccgcgcgtgc tcacgcagct tgctgcgaat ggtcatgtgt tgccgctaca gaacacgacc
     6301 ttcttcctga gcaggattac ctttttagca acccccaagc cggggatggc catctggcgg
     6361 gaaaaactgt ttgtggtgct gtcgcgcaat acccagcgcg ccagttctta cttccatctg
     6421 ccgtcggagc aggtggtcga gatcggattg gtactggaga tctagccatg cagcggaatc
     6481 tgttctgaga cgatccatct gtcgtgagga cggcaagggg cagattcgaa aaccatcaca
     6541 gacactgtcc gaactccgcc tgccgccagg cttcgtagag caccaccgcc accgaattgg
     6601 acaggttgag gctgcggctt ccaggcacca tcggcacacg aatgcgttgc tgatcggaga
     6661 cgcttgcaag aatctccgcg ggcagaccgc ggctttccgg cccgaacacg aacgcatcgc
     6721 cctctgcgta ttgaaccaga tcgtaccgct gccttccttt agtcgaaacg gcgaacagtc
     6781 tttgccctgt gaaacgctgg agacacgttt cccaggtctc atggacggcg agcgtggcga
     6841 attcgtggta gtcgagccct gcccggagga gttgcttgtc ctccagcgtg aatcccaacg
     6901 gtttcaccaa atgcagccgg gcaccggtat tggcgcacag ccggatgatg ttgccggtgt
     6961 tcggaggaat ttccggctga tacagaatga catcaaacat gatgacaggg ggatggatcg
     7021 gcggagatcc gcggctatga gccaagggga gagccagccg gttgcccgga caacttaccc
     7081 ctccagccaa ttaatgatca ggaacgaaag gcctgtacat cacccgattt gcgcagcatc
     7141 ttattcactt cgtcgagatg gagctcttcg cccacgatca tttcccgggc aaactcctcc
     7201 aacaagacgg atttaccctc cgccagcttc agtagttcgt aataagacgc gacggcggcc
     7261 ttctcatgct ccaggctctc ccgcagaata tcgccgatat cgtgtttttc ggtttcgagc
     7321 aaggtcccga tcttcagcga cggatggcca cccagcaggg tcaccagttc gccggccttg
     7381 tgagcatggg ccaagctctc atccgcgttg cccttcaacc acgacacgat cggaatacga
     7441 ttgtacccgt agatcatgag cgcatagtgc gtgtaccgca cgacacccgc cagttccagc
     7501 tccatgatcc ggttgagaat cgctaccgcg cgctgcgtat cctgttcgtt catcatcgat
     7561 ctccaatcag gcaggaattc actgcctcat aacatggtca tggcactaac ctagacgttg
     7621 aaacggaaat gcatcacgtc gccgtcgcgc acgacgtatt ccttcccttc cagccgcatc
     7681 ttgccctttt ccttcgcgcc cgcctccccc ttgcacgcaa tgtaatcatc gtagccgatg
     7741 acttcggccc ggatgaatcc cttttcgaag tcaccgtgaa tgacgcctgc ggcctgcggc
     7801 gcggtgtcgc cgatatgaat cgtccaggcg cgcacttctt tcaccccggc cgtaaagtac
     7861 gtttgcaggc cgaggagctg ataggccgcg cgaataaggc gattcagtcc cggctccttc
     7921 atcccgatat cggccaggaa ttcctgtttt tcctcgtcgg agagtacggc gatttccgac
     7981 tccagggctg cgcaaatggc aaccaccggc gcgccttcct gggccgcgta gtcttccaca
     8041 cgcgtcagga gcggattgtt ggcaaaccct ttttccgaca cgttggccac atacatcacc
     8101 ggtttcatcg tcagcaggca cagcggtttc agcagtgccc gttgcgccgg atccaacttc
     8161 attgtccgcg ccggtttgcc ctcgttcaga caagccgcga cctgcacaag caggtcgccc
     8221 aacttggcgg cgtctttgtc tcccgagcga atgagtttca cattccgctc ttgcgccttt
     8281 tccacggttg tgaggtcggc cagcgcgagc tcggtgacga tggtggcgat gtcagaaatc
     8341 ggatcgactt ttcccgccac atggaccacg ttatcgtcct caaagcagcg cacgacgttc
     8401 acaatcccgt cggtctcacg aatattggcg aggaactggt ttcccaaccc ttcgcccttg
     8461 gaagcccctg ccaccaatcc cgcgatatcc acgaactcgg tcgtggcgta ctgcatccgc
     8521 tgcggcttga cgatatcggc cagcgcctgc atccgcgcat ccggcacctc aacgatcccg
     8581 atattcggct cgatcgtgca gaacggataa ttctccgcgg cgatccccga tttggtcagc
     8641 gcattaaaga gggtggactt gcccacattc ggcagtccca cgattccaca tttcacactc
     8701 atgacgatcc cttcctggtc atggccctgt tgattcggcc cgccgttccc atatctctac
     8761 tgctgaaggg gcagcgtcaa ccgaagaggc gcattctacg tgttcaaggc tggtgcggtc
     8821 cactcccctg cactcggggc agaaatggac gagcctcaac tgcacgttca cattctcaat
     8881 gatcaataga gggatacgga gaagaccttg gaggcagctg acggacggcc gatgaatgga
     8941 gcacagatcc cgggcatgaa ccatatggcc catgcccggg atagatggag tcaacttgcc
     9001 gattgtgcag tcggcggatt atccccttcc ttccgatgaa ccagccgacg cgggcatagg
     9061 cggcaccact gcctcgggat tgcgaaacaa agccccggac gcctggatga tccagtggat
     9121 cattttggta tcgttctgct gaatatgaaa cccggttgtg gccatcaggc cataggcgtg
     9181 ccgggataag gcatagcggc tgaacttgcg cgtcgccgac tcctgtttcc aggtcgcacc
     9241 aatgacacga aacccttccc cttgcatgat gtcaatcgac ggaaaacttc cctgttcatc
     9301 gccatagtgc caatcacaca tcacaatatc ccgtggaagc tgctggcgta gcgctttgcc
     9361 gtatcccgcc gcgatgccat ggagatgcct ggtctgcgcg ccgggaaatt ccgccgggct
     9421 caacaccatg tccgcccaca tccaggtttc tacccccttc ttgccgagat aatcatgcac
     9481 ccgcaccaca tcccgcagaa agagatccgc cgggatcatc acttcaccca gcttcaacca
     9541 tttcgacacc tgaccgacgg tccatccgaa agcttcgtcg tggccgatat ggatcgcccg
     9601 cggatgcacc gcctcgatca gttcgtcgag aaacggaaat accaccgcat acgtcgcatc
     9661 tttccgcgga tcgtaggaca cggtattgaa catcaagccc ggatattggc gctgaaagaa
     9721 ttgctcctgg tgcgtgagca gcttgacctc tggaatgact tccaagccat gggcacgggc
     9781 ataatcggcc caggacagga actccgcttt cgtccaggcg gttttcacgg gtatccaggg
     9841 ggcatggtcg aactgcacac cgtcggtcag cagcacctgc accgcggtaa accctgcagc
     9901 ctgggcttca ttcacaaatc cgcgcgcgcg ttccgtcgac acagtccctg tcaggacgaa
     9961 ctgcatgatt cgcagggctt ctgccgcagg aaccgacgac ggctctgccg ccgcaccgac
    10021 ttcccataaa ccggcccaca tcacgaccat cgcacacaac aacatcccgg aacggcccat
    10081 gtgcgtctct ccatcacaat ggttcggtca gttgctttcc aggacggccg gccttggacc
    10141 ggcgcccttt cctcgccttc gaaggcagtc gttctcgctc atcacagcaa cgcaggaata
    10201 cgtagacaaa aaatcaggcg ggcaaaatac accgagccat gcatcacgtc aagaattcag
    10261 gacagagaaa agccgtcggc acagagattt tctccgcgat gtagatcaac gtaggacaac
    10321 gcaatggaat ccaactacag gaatggccga tttgaccgcc tcagggtatg gggaccggcc
    10381 gctatcggag atcgcgttgg cagcacaggc gagagcaaca cgagtggaac ggtactgtgc
    10441 accgctctac accggcgagg aaaagtgaag aggattccct acctgtcatc ctgatgtacg
    10501 ctcgccggaa tgagcaaatc gcttgggatc cgttccggtt caccatacac tgacggcatg
    10561 cacagacagg cttgaggaga ggagcaccta tggatagatc acaagccggc accgtccgat
    10621 cgatgtcttt tgcgtgtgtg ggtctgaccc tggtgtcgct gttgaccgga gcgctaccgg
    10681 cggcagcact ggaaagcagt gctcgtacaa tcgtctccca atcgaacagt catgtgggac
    10741 tctccatcga tctcgccggc ttcaccattg aacgcgatca actcaaaccc aacgggcgcc
    10801 gctacctcat ggcctcccat ccaggcacag gcatcaaggt cgccatcgcg ttggagcagg
    10861 tggccggaaa ggcttcgacc gccggatgca tcgagcattt gcaacacgtg cagaaaggat
    10921 cagcggtgag ccgaggaaaa gacgtcactc tttcgactgc acacgagatg ccgacgctcg
    10981 aatacacgct cccccgcttt cagggtgttc gtctcgatca aaagagtttg tacgcctgca
    11041 tggccgaagg gaacgtctat gccaacatcc atgtctcaaa ggtccggtat accgatgccg
    11101 acgcctcccg ctttcggcag ctgctgacca ccctgcgctt gcaatccggc ccatcgccac
    11161 ggatgacggc tcaccaatcg aacagcgccg cgccgctgtt ttccgtggac accgtgcgat
    11221 acgagcagaa gaacgaggcg agaaccattg cgccgtaagt gagacgccgc ttaccggggc
    11281 gccgtgaggg catcctgagc cgtgtgaccg ctcagggatt tttcctgaat gttgtgatgg
    11341 atctccagag caatgacatg cgccagttga tccgctgata gggctcccat cggcagcccc
    11401 tgcgtctccc gataaaaggg gtcatctgca gcagcgaggc ggtggacatt gaccagaatc
    11461 gccgccggtt gtatgcgccg ctcccgtttt cccaccacca cagacttttc ctcggacaac
    11521 accctcgtgc gggtcgacca gtcgcgttga tcgattttca cataggcgac attccagact
    11581 ttgccattga gaagattgcg gaccgccttc ggggttcgct ccagaaaaaa cgggtcacgc
    11641 acgatcttct gcagcgcttc acgaatgcgg atgtcataca acttcgtggt tgaatccgca
    11701 tgagtcggcg gtagcggcgc ggaatggccg acttcgtacc agggttgtcc gtcgaggctc
    11761 ctctgatcgt ctaccgttcg cagataggtg tcgaagagtt gttggagcaa ccgaacccgt
    11821 tcttcggcag agagacccgc aatctcttcg tccgtgagga tgggggcctt ctccaggttt
    11881 cgttccacgg ccaccgcctt cgcctttggt gcggtatcgg ccttgctcac cacccactga
    11941 aactctctgg acagcacagg cagcagggaa tccggatgat tgagataccc tctgtccttc
    12001 agggaggtgg cgctgatcag cagtgtgaca cggcccggtt ctttcgtgcg ggcgaccaaa
    12061 aacggaaagg ctttcccctc gtcattgacc acgaccggtt cgattacgac ctgcttgagc
    12121 gcctgtttcg tgaggctctc ctcaaaacgc ggatagtcgg cacgatgctg cagcataatg
    12181 atgaaggtgt cgattactgt ctggacagcc gcatcgagat cggcatcatc cagtccgctg
    12241 acatcggcta ctgcacctgt gctcagaaag actttaaaag gtgcacccgg gatttctcga
    12301 ccctggccga ggacaccggg cacttccgcg tctgcatggg gttgatgatc gcggtgattc
    12361 gcctgcgcgg ccggtacaaa caggaacgcg ctcaggagcg caccggcgcc aagaacccag
    12421 accacagatc gatgacggtg ggtgagcaga tctgtttgcg cgcgatctct tgtgagccgg
    12481 ttcatcatcc gcaatcagct caacctgtgt atccgttcgg attgttgtat tgccagcacc
    12541 aggcatcggt acacatatca tccagtccac gggcggcctg ccaccctaac gctttgaggg
    12601 cataccctgg atcggcgtaa caggaggcga catcgccggg acggcgctgc gcaatcttgt
    12661 agggaaccgc cttcccgctg gctttctcaa acgcccggac aatctccagc acgctgtacc
    12721 cgttccccgt gccgagattg accgtcagac attccttcgg gcggtcccat ccatccagcg
    12781 ccttcagcgc tttgagatgc ccgagcgcca aatccaccac atggatgtaa tcgcgcacgc
    12841 ccgttccgtc cggggtcgaa taatcgttgc cccacacatt cagatggtcg cgccggccga
    12901 ccgccacctg cgccacaaac gggagaaggt tattcggcat gccttgcgga tcctctccaa
    12961 tcaggccgct ggcatgcgcc ccgaccgggt tgaaataccg caggatgccg atccgccagg
    13021 agtgatggct acgctgcaga tcgcgcagga tctcttccac catcaatttc gtacggccat
    13081 agggattggt cgcggacagc ggatgatctt cggtcaacgg caagcgttgc gggtctccat
    13141 agacggtggc cgacgaactg aagacgagtg tcgtgacgcc gcactccccc atcgcctcga
    13201 gaagacgcaa cgatcccacc acgttgttgt cgtaatagga cagcggcttt tcgaccgatt
    13261 cacccaccgc tttcaacccg gcaaagtgga tgaccgaggt ggcgccgctc tcccgaagcg
    13321 ccgcaacgag agccgcccga tcccgaatgt cgccccgtat cagacgaaga gattttcccg
    13381 tgatgcgttg cacacgcgcc agggcttcag gatggctatt ggaaaagttg tcgaacacgg
    13441 tcactgccga gccggcattg agcagttcca cacaagtatg cgaaccgata tacccggcgc
    13501 cccccgtcac caagatcatg cgcatcgcct ccgctgggat gagatgagaa cacctgcctc
    13561 taccggccta cgatacaccg cctcgaaggc tcgcacaatg gcacggccgg aatcacatgg
    13621 ccttccttat ttcaatctag ccgtttcctg tcgcactccg ttacacttcg aacacgcagg
    13681 gatacccatg cagcaccaac acaacaccaa ctattacgcc atcctggaat tgtctgctga
    13741 cgcgacggag gctgatatta aacgcgcctg gcatgaacag atgcagatct ggcatcccga
    13801 ccgcttccta cattcatcca ccctgcacaa gaaggccgaa gctcgcacgc agttgatcaa
    13861 tcaggcctat caaacactca gcgatcccac agcgcgcgcc cgctacgata gcggaaggca
    13921 acagccctca gcaccgccgc ctcccccgcg accttcttcg cccccacgtc cccaaccggt
    13981 gacacgcccg cgacaggagc tacgcggacc gcaaacgatg ctgaacgtca cccggttcag
    14041 tcatcccaaa atcatggtcc ctgccattca tcttctggtc gacgtgcacg aaacccaacc
    14101 ctacgcattc aaagggctca ttcggatcgc gggaacgcgc acgcaggccc tgccggctgg
    14161 agattacgcc atcgccgaag cgcccggcat cttttgtgtc gaacgccgac gtgcggagga
    14221 gtttaatacg attgtctcaa atccgtccga taatcgcccg cgtttcctcc gcgaactcga
    14281 gccactgctc gcgatccccc atcgttttct cgtcatcgaa ggcgccctgc attctcatcg
    14341 cggtgggggc cggctcgaac agtatcatcg caatggcttg atcgactttc tcgattccat
    14401 taccgcacga ttcggcctgc agatcattta tgcagacagt cgcgacgagg ccgaggagag
    14461 agtcgcgaat ttggcagcca tccactacgc ctattacctt gccgaacaac agggtctcgg
    14521 tcgctgcctc acggagaacg acgcctaagt ctgaaaaaag gggcgagtca ctgtgcgggt
    14581 gatttagaga acgtacctgt agccgaccta acaatcctac tcgttgttat cacttccagc
    14641 cattgatggt tatgcaggcg gcacgacatt atgtaactca tccagcttga gacgcttaag
    14701 ccattcttga ggagtatgcg cgaacacctt tgttgttcgt gcatgcgaga tgatgatgtc
    14761 gtagccatac gaaatcacta tcaccacttg cccaacatca ccaactccgg acgaagaccg
    14821 aaacatcgcc atagcgatgt tgtctgcaac actttcgaaa ttttccggtt ttacacgcac
    14881 ctccacacta agctgcaccc gggaagcggg ggtctgcccg ctcccaggag tcgtcatcat
    14941 ggaagtctgc cgactgatcg acacatgcgc atccggtacc accgattgag ccaggcgtcg
    15001 cgcctctgcc agctgcgata caaaatcagg cggatcagag gtgtaaggca gcagcaagac
    15061 cccgagcact agagtgctga gtccaccggc aatcgcgaga tgaggcttcc acatcttctg
    15121 ttgaatctct gaagcgctct ccgcctccct cacaaatgtt ccaaccacga tatcgtgaag
    15181 ggcttgacct gtccgtcgat tgcaacagta gagatatgcc tgtgccacac ctacaccaaa
    15241 gatcagaata ctgaggacaa tgcccacaag ctgttcatat ggtcctggag gagtggctgt
    15301 tccattaagt gccaatggtg cgaaaaagat tgttgcacgg aaagccgacc ggggcaggct
    15361 aatcagtgcg cccttcgcat ctgttacccg gattttcgcg aggcgtttac caagagtctg
    15421 cccctggcca atttgactat tcaatgtggc gaaatacacc aacgcaattg cagcaccaat
    15481 gaatcgaccc gttcgtccaa gcgcggctaa ttgatcgaac cacaggaaac caagacaagc
    15541 cccgatagct ccaaggagta acacgtccgc cacagcggct ccggtacgtc gccagaatgt
    15601 tgcgaccgga gttgcccgcg cttcttcgtc tttgcaggaa ttttccaagt caggtgtcat
    15661 atgaattctc ttccagagtt ttactttacc tggtgattgt tgactcactg gaatcacctg
    15721 tcaatttgat ccgcacaaca tgaatgcacg atcactggga cttccccgct acgtaccatc
    15781 catttctatc cctgctatcg tttcccactc ccaccacaat cgcccctttt cttggtgagg
    15841 cgtggggccc gtgatagcat ttcattccta tgccgctctc aaccaccgtg cacgatctcc
    15901 gcaccctgat tcgctcctct catccactca ttgtgatcga gaccgtggaa gaagaccggg
    15961 tgatgacgtt gctgcaatcc gtcgccgccc aggaacgcat gcccctgttc gaatggtcga
    16021 ttacgagagg cctcaccagg cccgaggaag ggccgagcct cagcaagctg accgccaccc
    16081 cactggcggc gcttcaacat ctcaatggat tgaccgtcga aggcattttc tggttgaagg
    16141 atcttgcccc gcacttgcag gatgccgctg tcgcgcgcca actgcgagag gtgagtcatc
    16201 acctcagccg ttcacgggca acctgcgtca tgacgggaca tcccatcacc ctgccgctcg
    16261 acctcgatca gattgcggtg cggttcgatc tgcagctccc ggatcgagat gagttacacg
    16321 cgatgcttca gagcgtcctc caatcgttga ggacgcgaac gtcaccccgt cgaccaacct
    16381 cgaccaccgt cgtgcagagt ctgctcaact ccatggccac ccccaagccc acgatggccg
    16441 gcccatcgga ccaggattgc gaggccatcc ttcgcgcctt acaaggcctg accttgcatc
    16501 aggcacggca ggtcatcacc caatgcgtga tcgaagatgg agtgctgtcc gccgacgatg
    16561 tgcagacgat tctcacgcgc aaagtccaag ccataagaga cggcggcctg ctggaatatt
    16621 atccgttgga agacaaccgg ttcgaactgg gcggctttgt gaaactgaag tcctggctgg
    16681 agcgggcgaa gatgggcttc acggccgaag ccaaggcgct caacctgacc ccgccacgcg
    16741 gcatcatgct ggtcggtgtg ccgggatgcg gcaaatcgct cgcggccaaa gccattgcgc
    16801 gcgaatggca cttgccgctg ctcaaattgg atgcaggccg gttattcgac aaattcgtcg
    16861 gcgaatcgga aaagaacttt cgcaaagcga tcgagatggc tgaatccctg tctccgatcg
    16921 tgctctggat cgatgaaatc gaaaaggcga tgtcggcggg aggcggcagc ggagatgcgg
    16981 atgggggcct gagccgccgg ctgttcggcg cctttctcac atggctgcag gaaaagaaac
    17041 aggacgtctt tgtcgtggcg acagccaata atctctcggc tctgcctccc gagttgctgc
    17101 gcaaaggccg ctttgacgag atattctttg tggatcttcc agacaaggac gaacgtaccg
    17161 ccatctggtc gatccatctg aaccttcgca aacaagaccg cgcacaattc gatctcccga
    17221 agctcgtgga cgccagcaaa ggattcagcg gatcggaaat cgaacaggcc gtggtcgctg
    17281 gcctatatcg tgcgctgtat cacaaacagc cgttgacgac cgatcttctc atccaggaac
    17341 tctcccatac cgtgccgctt tcggtgacgc ggagtgagga gatcaatcag ctgcgagcca
    17401 ccgcgcaagg ccgcttcgtc aacgttcgat gagcgggttc cgctcttcgt cgttctgatc
    17461 gggccacttt ctctcggcgc ccacacggca tcactttcac ataatggcgt ggtcccccgg
    17521 tgcgctcgca gggctagacg ttcatacagt aagatagagt cttatgtctc gatccatcag
    17581 gcgaaggcga atccttcaga ctcgttctgc ctctaatgat cgaaagacag gagacggctc
    17641 gtccgtaaca ctagagacat ccaccatgat cacacataga tttcgtatgg ctgttcccaa
    17701 agaccggcag gttcgccacc atgggcggct gctcagccta tcggtcatgt tgcttctcac
    17761 gcccattgcg ggaaccggtt cgggcgcgtt cgccaatgac ctcccgcttc gctcggaagg
    17821 ccagatcgca caacatgcca aggccgcctg ggaacagggg acgctcaatc ttgcgctcgg
    17881 catcgtcgag cagggcattc aagaccatcc gcgagcgctc gcgctgcatc aacttcgcgg
    17941 agacatgctg gccacctcac ggcaaaccga agccgcgctt gaatcgtacg agactgtcct
    18001 ggcaagcatc cctgccgccc tcgacgtccg gtgggccaag tggagtgtgc tggtgagatc
    18061 aggacaggtc gaggaagcgg tcgccgagct tcaacgcatc gccgtaatcg atccgcacaa
    18121 ccccctgctc catctgcgcc tggctcgcga actccggaaa ctggaccggc tggaagaatc
    18181 cttcgaggcc taccagaaag ccgtggaact cgggccggac atgctcaact ggcggttggc
    18241 gctggcgcgg gcccggtttg atgttctcga ttacgaaggc gccgaacgtg acgtgcagtt
    18301 cgtcctggat cgagtcccgc ccggctcgcc gcttcacctc tctgccagaa atctcctcac
    18361 cgtcttcttc ggctcgacgg agcgggggcg ccgatttgca ccggtcatga cgccggatgt
    18421 cagcccgaaa caattacgcg actggtcgat gatccgcgcg gaggggtacc ggctctttga
    18481 ggcgggacgg tttcgtgaag cggagccggt ctatcgagaa cttcttcgcc tcaacccgat
    18541 ggatccgctg gccgggcggc atctcagtct catcctcatg gggttgggcc gttgcaagga
    18601 cgcgctcgca gtgtcacaag ctgggtctga attcgatccg actgatgaag aacatacgac
    18661 cacggcgttt cgtatgggcc aatgcatggt ggagctggga cgctgggaag acgcctacat
    18721 gcagttcaag acgctgtacg atgcgaccgt ggagtttgaa aaatccacga aggacgtgca
    18781 gctcccctcc ggaacccgtg tcttgaataa ggccaacctc gtacagtggt tggagaaggt
    18841 ccgcctgcac gtgccggagt ttgcccagca ggtcgacgaa gacgctgcag ccacggcggc
    18901 tcaagccaag gcggcggctc cgacgattcc tccggaggcg gaactcgccg cgaaggccat
    18961 cgaagctttg aagccgcaaa acacgctgga tcctgcggcg tccctcgtcg gccgggacgc
    19021 cgacttcgcc tggtttcgtt tcgtcattcc agcaaaaaaa gtaatgcgcg atgattcacc
    19081 gaccggcgcg catgacttca ttccgttgga cccgggtatc agcttcccgg gcagtcagca
    19141 ggatatctat ctggtcttca gcctggtcac cgcctcatat gatgaggtgc ccctggccgc
    19201 acgctgcttc aaagagaccg ccgaactggc cggcgcacac acagccgtcg cgcaagaccg
    19261 gctcatcatg tccacgaacg atcaatccgg ttattttgtg ctgcctcgtc cggccacagg
    19321 ctggacagcc ggcctctacc ggtgcggcct gtttgcaggc gaagaaacgt cggcctatac
    19381 ccaggttgat gaggtgcggt tccgcatcat cgaacccacg cggccttcgt aacagacagc
    19441 cgtgatattg gaactggtgc gctgcaccag aaagccgcac cgtcatcacc ggctggaaca
    19501 gatcaacggc tgcgtgccct gacggagtag gaaccggtga agacctcacc gggaagcagt
    19561 cgcttgagtc cccagtcggg gtgattaaag gcgtcggtcg cgcaagtcat cggttcgatg
    19621 gccagggcgc ggcgaggcgc atcagcgatg gcatcccccg tgtagaccac gaccgccgag
    19681 aaagcacgat ccatcaccac atcaatcgtg cgtccacttt tgtgattgcg cagtgatgcc
    19741 gtggccatcc ccgccgcatc gcgctctaat tcaagataac aatggttgaa gcggcgcgga
    19801 ccaatgcgct gaaacgtgcg gtagtcccat tcagttcctg ttgcggcgat caccttgccg
    19861 gtgggcgcga gctgctcgtt gaattcgaga aagcctgcgg caggaatttt ggcctcagcc
    19921 tcatcgatca gcccggtgcc gaccgtgaag tagggatgga atcctactcc gacgggagct
    19981 gccgcacctc ccacattccg tacagaaaat gtgcaggaca atccctgaat gcccagactg
    20041 taggtgagac gcacgacgag cgaaaacggg taccctcgcg aggtgtagtc ggcggcatcc
    20101 acacttactt cataggtcac ggcgtgagcg acggcttgat gcgtcgtcca tgggaggctt
    20161 cgcacaaatc cgtgaatggc attcgggcct tccttatcat tccgttcaag ttgaagtagt
    20221 tgaccgtcaa acgagtatcg tccgcccgcc acgcgacctg gaaatggaat cagcacatcg
    20281 ccctgcccgc ccttcttctg cccgccgcct gaatagcccc atacgatgtc cgtttcgctt
    20341 ccagaactgc cgacgataaa gtaccgccgc aaggaggcgc cccacggtga cagcaccgca
    20401 cgctggttct caaaggcgat cgtcgtttcc gtgtcgggcg tgggggcaga catcatgttc
    20461 tgcaacactc cttacacatc cttaccgatc aagtactcga accgtttacg aaaatcatcc
    20521 gaggcgagtc cggcggtggt cgccaatcga accaggtcat ccggttggtg cagatcgtcg
    20581 gacgaaaggc ggatcatgta ccgacgtgcg atgacgtaag actccgactc gacgtccacc
    20641 atacgcacgc gcgtacggcc ggtggccggg tcgagaatgt cgccaaacgg aataggaata
    20701 aaccgcccgt tttgaatcga caccatggcc gcattgccgc cttccaacag aaattgcgcg
    20761 gcgcagtagc ccaggtcccg cgtatattcc atatcgaatg aaataggatc cgcgcagcgc
    20821 agttcatacc cgatattttt atccacgatg gtagtcttga tcccgaatgt acgcagttcc
    20881 ttctccaccg cccgcttgag cacgaagccg aaattaatct ccgccagccg cacatgtccg
    20941 tgctggtcgc gttccacatc ttcgagtccg ttcaaatcct gttggtcgag aatttcgacc
    21001 aggccttcgg cgagaaccgc caccccgtcc gacctgccgg cactgagccg cttgataaac
    21061 gcggccacca gaatatcgac cagcgtctgg agacgaatgg gtttgtggcg gaattcttcc
    21121 ggaatgatgg tcagggtggc tccggcagcc ttgccgatcc ccagcgccag gtgcccggcc
    21181 ttgcggccca tggtgacaac gaaataccag cgggaggtgg tctccgcatc gaccatcaga
    21241 ctcttgacca gttcgacccc gatatggcga gccgtctgga atccgaaggt cggaatgcca
    21301 tgcggcagat cgagatcgtt gtcgatggtc ttgggcacgt gcaccacctg aaggcggccg
    21361 ccggccctct gctccaactt caaggcggag aatgccgtat catctccgcc gatcgtaatc
    21421 agtctggtca cgcccagcgt atgcagactt gccaggcagg catcgaggtg ctcggttttc
    21481 tgcgtaggat tggcacgcgc cgtcccgaga caggaacctc cgctgaagtg aatgcgactg
    21541 atgtcttcga tcgacagacg tctgaccttg ctggtgttcc cgtccatgat ccatttgaaa
    21601 ccgtcctgaa tgccaatgac gtcggagccg ccgagaatgc ttcgtatcgt cgccgccccg
    21661 atcacactgt tgatgcccgg tgccggcccc ccaccgacaa gaatgcccac gaccggtcgc
    21721 tgttcacgta tcgccatgtt cctgctccta tcggtctgaa atgattcgac ccgctccctg
    21781 cgccgtcagc gcaccatgct aggctgtttc cggctatccc gcaggccgtt catgcctgcg
    21841 accctacacg cactcagaaa gtgcgtcaag gacgaccagg tgggttcttc aatatcactc
    21901 gcgaattatc gaacctgtgc tagcctaaga ccgacagcct tctgcttcac tcgaaggagg
    21961 tacttcatgg tcgagatact cgtcgtcgac gacaatctgc aactgagaga caccttacga
    22021 aagatcctga acaagcggcc cggctttcgt gtgaccgggg aagcagccga cggcatcgcc
    22081 gcctcgcaat tggcccagac gctccgcccg acagtggtcc tcatggacgt aggcatggca
    22141 cgcatggatg gggttgaggc gacgcgccgg atacgcgcgc tcctgccgaa cacggtcgtg
    22201 attggaatgt cttgccataa cagccgggaa gtcgaggtcg ccttacgcgc cgtcggcgcg
    22261 aacgcgttca ttcccaaaga gctggtaccc gaacaaatct ttgacgtcat ctcccagcaa
    22321 ctgccgcagg atcagacggt gcaatgatgc ggtggaagaa gcagccctac agcttcttct
    22381 catgaggggc cacaactcac atctctacct tctccgctcg cccctgacgg ccggctaagg
    22441 gccgcctcga caatcgctcg gcacagctgg acaccagcgc tttctttggt gagataccca
    22501 tacgctcccg cttgcttcat gcgtgccacg atatcgggcg agttgtgcat ggagagtgcc
    22561 acgattttta tttcggggta ttcttccttg atccgtttgg tcgccaccac gccgtcacat
    22621 cggggcatgt tgacgtccat caccacgaca gcggggctga gggtacgggt caattcgatg
    22681 gcttccaggc catcgcgggc ttcaccgacg acctcgaggc cgggatgtcc gttcacgatg
    22741 gtccgcagcc cctggcgcaa cattcgatgg tcgtctacca acaagacccg gatcttggga
    22801 gtggcgagga gcgaggcagt tgtggggagt ttcggcaccc ccggtgcggc cggctcggac
    22861 accacggcgt gactcaccgc agcgacaccc gtttcagcca ccgcctcatc agccgtgggg
    22921 cctggctgaa caaccggcag ctggagaatg gccgtcgtgc cctcgccggg gactgattgc
    22981 atgtcgaact cccctccaag agccttcatg cgctcgcgaa tactgaacag accaaagcct
    23041 ggttccagct tggcctcccc cggaccgacg tcacaaccca caccgcgatc cgacacgcaa
    23101 atgcgcaata ctccgtcggc ctgtgtgacg gaaatcttcg cctcagtcgt ccccgcatgt
    23161 ttcacgatat tcatcaggag ttcgcggacc gattgaaaca acaggacgga ctgctcctcg
    23221 gaaagggaga ggacgtcact ctccagatcg agcttcacgg tgagctcccg ctgcatctgg
    23281 tccgccagcc atctcagcgc catcaggagt ccgaactcgt ttaagatcgg cgggctgagc
    23341 tgtgccacca acgtcctggt ataggccagg gcccgatcca tcacttcctg gacctccgtc
    23401 atggcctcca tcgccgccgg cgtcacctct tccaatttgg cctgcccgag tttaatccga
    23461 ctcaacacca ggagttgcgc cagatagtcg tgcaagtcgg tcgcgagttg ttttcggata
    23521 cgatgctcag tcagattcag ttctgtcgtc agcgcgcgca gcttgtcctg cgtatccgcc
    23581 aattctctcg tccgcacctc gacacgctgc tccaaatcgt ccgccagttg gcgcaattgc
    23641 gcttgtgctt ctttccggat ctgaatgtcc tgaatgatgg aaatgaagtg gtgcgcggag
    23701 ccctgctcat cccgcaacag cgagacggtc agatgccccc agatgagcga gccatccttc
    23761 cggatgtacc gtttttccat ggagaaggtt tcgatctcac cggccagcag cctgcggacg
    23821 gaagcccaat ccccttcgat gtcatccgga tgggtaattt cggtaaatgt cctggcgagc
    23881 aactcttccc gagagtaccc cgtgagggcg cacagggcat cattgcagcg gagccagcgt
    23941 ccatcgagcc ccacatgggc aatccccacc gccgcgttat cgaaggtggc gcgatgttgg
    24001 gcctcgcttt cccgtaaggc atcctcggcc tttttgctgt ccgtgatatc ttcggcgaag
    24061 ataatgatcc cgccgatacc gccggccttg tcccgccagg gccggacttc ccatcgcagc
    24121 caatagacgg tcccgtcgga acgttcgaac cggtcttctt caatacgcat cacgacgccg
    24181 gccaagccct cctgatgcgc ccttcgccac cgctccggaa tgtcgggaaa aatctcgtag
    24241 tgactgcggc ccaacacctc gccttcaagt ccgtagtccg ccagccagcg gcggctcacc
    24301 gcgacatacc gcatctgccg atcgaacatg gcgattgcgg cgggcgcatg ttcgatgaag
    24361 agccgcagcc gttcctcact ttcccgcaac gccgcctcgg ctcgcttgcg ttcggtgata
    24421 tcgtgggcga ccttggagac gccgacgatc cgtccctcat cattgcggat cgccgatacg
    24481 gtcagggcaa tatcgatcaa cgtcccgtct ttccgccgcc ggatcgtttc atattgctga
    24541 accgtctcgc ctcgcacgat cttcgcaaga atcggggttt cctcctcgat ctgttcgtcc
    24601 gggatgacca gcagcacgga ttgccccacg gcctcctgcc gggtataccc gtacaaccgc
    24661 tcggcacctc gattccaact ggtgatgagg ccgttgagat ctttgctgac gatcgcgtcc
    24721 tctgacgatt cgacaatggc ggcaagcgtg gcccgtgcct gctcggcctg tttccgttcg
    24781 gtgatatcga cgatggcggc aatgaatccc gtcacgatcc cgcgttcgtc ccactccggt
    24841 gcgtaggtca cttgcagaag gcggcctatg gacgcagcac caagaaattg ctgatcaaac
    24901 ttgacctgcc gtccagccag cgcctctcgc atatagggtt cggcctgctg gtacgcctct
    24961 gcgcccaaca cctcacgcgg atgcaggccg atgagatctt cgggcgtccg tcgcacgagg
    25021 tcggcatatc gctgattcac gaaccgatac cgtccatccg ttccgcattg tgcgatgaga
    25081 accggagcat gatccgtgac gaactgcatt tgacgctcac tggcgcggac tgtctcctcg
    25141 gccttggcgc gcgcgtcgat ctccagacgc aatcgctgtt cctgggccct caccagggat
    25201 gtctccgcct gcgcccgaag acgagcacgc gtgaccgctt cggagagcga cacgatgatg
    25261 acattggcgg tgataaacaa cgccagcgcc gtctgctgct cacccggcga agcctgcata
    25321 tcaaaaaaca ggacgatagt caggactgag gagaccaggg tggcggcaac accatgtcgc
    25381 cagccattgg ccacggcgat gaacatgatg gcgggatagt acgtgatgaa ggtatagcct
    25441 gatccccaca gcggagtcag cgccatacga agaccggtcg ccacggcgag cgtgaccgtc
    25501 gtgagcagtg aaggattcca ctcggccaga cgcgcccacc aggagtgacc accctgctgg
    25561 atcccgtcct gagatgaaga agctgaagga ggcgggtcag aagccatggg ctccatcggc
    25621 gagcgatgaa tcggctcttg gtctaggtgg cgatcagaat gatcgtgcgc agcatgcgca
    25681 ggatgttcgg ctaagaggcg tagtgtacct gaatgcctca ggagacacca cacctgatcc
    25741 tggacattcg gggaagcgag atggggctag cggggcggac gctgaagaag caggtatgct
    25801 agttgtcgtg acgtggcgat gcaggcgagg cactattccg gtgacggttc tccagccatt
    25861 cgatcaattc cattcgtaat gtccggttga gccgttgctg agcttcaaga ggcacgcgca
    25921 cgaactccac gccgaattga ttcccccgca cccatttgat cgtcccgagt tcaaccggca
    25981 acgcgggcgg ctgatgatga agaagcatac tcacccgcac ctggctcccg cagagcactt
    26041 cgcgatcgca ggtcaccgag cagcctttta atgagagatt ggtcaggacg ccttcaccga
    26101 caaagggcgc tccgccaaaa atgactggat agttgagcgg aaaccggtaa taggttcgag
    26161 cataccgacg aggtgccatc acaacctccc tgtggcggga tcctaccgag gctcgccggt
    26221 caggcggtat cctaccttgg gaggggggtt ggcgactggt cttccttcgg tatcggcagt
    26281 ctccgtctcg tctgccctgc ttgtatttcg agagggacct gagtagggtt cgccccatca
    26341 cgccagagaa aggacactat gcacaagtcg ttacgccatg cagcctggat catcgcgatc
    26401 gccatgctcc caggctgcgt cggactgggc gcctggaccc tcggcacgcg cagcgaatcc
    26461 agcgaccacc cgaagatcga tcagaccaga ggtgccatcg atctgcgcaa ggcaggcacg
    26521 ccctctgagt tcaagacggt cgctgaactg cgaacgcaat ggggtgagcc tgacaatatc
    26581 gaattacgca aggatggacg ggaagagtgg atctataaga ccggcggctt acgatggtcc
    26641 ggtatggtgc tgtatcttgt tgtcatcccc ctgcctgcca tgatccctgt cggcagtcaa
    26701 tacgtctctc ttgtcattca cgatggccgc atcgaacaag ccacccgcag agattgggcc
    26761 ttcaaggctg gggcctattg cggattcttc gggatggtgt acggtggatt agggtgtgga
    26821 actggcacgt ttgaagagaa acagggatcg gacgcccagg tagtaaacgg aactccgtga
    26881 agtcccggcc catccctctg cccgatcatc caccggacag agggatggtg aaggtctccc
    26941 gcctactgag ccaggtcagt gaccgccgcc ggatttttcc ggatgcacgt caataatgct
    27001 gccggcttca ttcaactcag cccggacgcg cgcgccatcc ttgatggaag aagagagttc
    27061 ctgcttttcc atcgcgaact tttgctcgcc cttcgaggtc ttcaacgtga tctcgcgctg
    27121 catgacgcca atcgatacca cctcgcccgt gataaaccga tgttttcctt cagttccttt
    27181 cgggtgcact tcgagcacgt tattgttctc gtcgatgacc acggtgactt cgtctcctgc
    27241 cttgggaagg gcatgcccat gacgctcgga gcggttttca ttgagctgat aggtgctgcc
    27301 attgggaact ttcactgcaa gtgctccccc tttttcgatc acgaccccct gtagatgctg
    27361 atggtggctt ttgctggagc cctcatcggc ccgaacaaac tgaccacccg gtccgacaag
    27421 agccagcacg acgatagcct ggatgagaaa tttggacata ttcgaccctc ctgttgatgg
    27481 accacttcag cggttctgcg ctgcccccgt gaaaagccca ttacagaaaa gaatatcgat
    27541 gccggtgaga cgaatggact aggagaagcc cttgagttcc ctggcagaca aacaagattt
    27601 cctacaggct tacagagcga gagcatgcat cactcggccc gagtcttatg ttcgtccgcg
    27661 cgactgaaca acaggctaaa agagaaactg catcgtcagc ctggtttgcc actccgctcc
    27721 cagatccggc ctgatgacat gataataggc ttgcgccgtg aggctcatgg gctggtggtc
    27781 caggataaac gcccgcccca ctccgccgcc aaccgggacc agccacttat tgcccggctg
    27841 cgcttcccag ttcgccgtca tgatgggagc cgaggtgaga aaccagccac cgctcaagtt
    27901 gtagctggca aacggctgca tgagaaactg gctgacgccc gggtgatgac ggcctccggc
    27961 aaaagaccat tgctgacgaa gcaggacacc gcccaccacc ggactgaatc gaaacatcac
    28021 cacggccgat ggcccggcac tccacttctt actgccgagt tcttccatcg tcgaggtcgg
    28081 cagcgtgatg gatggcccca ggccccagga tacggccccg gtgcgaccgg gcgtgagaaa
    28141 cggggtgaaa ttgatgtcac ccaatccggt cgccgtgcca ccgcccgtgc ccgggatttc
    28201 atcttccaaa cccggagtga accgtgacac tgaaatcacg ggtatgaccg tgcgcgtgac
    28261 gacatgccaa tcacccaagc gaaccggcac aacgggagag atgttcaaca ggtgcgatgt
    28321 ggcgttgccc ggcccgacac cgaagaaggt gttgtactga aacggtactg agacgacatc
    28381 cgtcagaggc gtctggaact ttttcgcgag ttcctcagta gctcccatct ctgcccaact
    28441 cgatgatgcg cagaacagca tgacgagcaa cgcggcagac cacggaggaa gccaaccgaa
    28501 gctttgagac ctcgccctgt ctctatggaa cagtgatatc accggtgaga gacgactggt
    28561 aagcagcgag gaccggatgc acgcgtgtaa cgcgacgact ctgcactgtt ctcattcata
    28621 actacacata ccgttgtttt ttgtactgag tgagattgtt cgaacaggcc atgtcgtgcc
    28681 gactggccgg cgcattctcc ctattttttc cgtacgtggc aagccgtgtg gttcaacttt
    28741 gtgacaggcg gaaatggcga gcgtacagga tgggcgatgt gcgggcggtt aggcgtctcc
    28801 tgatcgcggc aacttgcgct aactgcggtt ccctccctct gtcactccca cggttcgaat
    28861 ccttcctcca aaccaggcgc tgccccaggc caacacaccc atgactccgc cgattccgag
    28921 gacgctggta atcgagccaa acgactccgc tacccagccg aaggcgctca tccccagcat
    28981 ggaggtcgcg gtggccatgg ctgtgtacac ggccatcacg cggccgacca tgccgggcgg
    29041 agccacttct tgaatgatgc cccaggcgat cggggtccag gtgcccaggc cgattccaat
    29101 acagactaca taagccgacg cgacgcccac actatcggtc catgtcagcc ccactaccgc
    29161 cagacctgcc agtgcacagg acaccgcaat tgccaacagc cgtttgtgaa gatcccaggc
    29221 gctcaaccag atcaggccta tggatgagag gagcagcccc actccgagcc aagaccagag
    29281 atatcccacc tcgaccgggc caaggccgag cagttggcgt ccaaatacgg gaaacagtgt
    29341 cgtgaatgcg ccactgccga acgtgtagat ggaggcgagg acgatcagga tcagcagcgt
    29401 gcgatgagtc agaaaggcat accgcaggcc ttcgaggaga tgccgaatga acggtctcca
    29461 gcccgcactg gagtctggtg cctgctccgt cgctcgcaac cggatcggca acaagcaggc
    29521 tgcggatgcg aaataggtga gcgcattcac acacagcaca tcctgcgatc ccgagaacgc
    29581 aataccgagt ccgctgacag caggcccgat gatgataccc agactggtcg tgctttgcag
    29641 cagcgcattg gctgccgtga actgcgggcg agccaccatg aagggaacgg cggatgacag
    29701 cgtcggcaca aatacggccg tagccatgcc gaacaggaat gtcaggacgt agagggattc
    29761 gaccgtgaaa tgttcgatcg gcaccagaca gggaatcagg ccgatcaaca gacctcttgc
    29821 gacatcgctc gtgatcaaaa tcgctttctt gggaaaccga tcgacgagga cgccgatgaa
    29881 cggcccgaag agtatcggcg gcagtgtctg gaggagccca atgaccgtcg ttttgagggg
    29941 cgaccccgtc accgcataga cgaaccacaa cagcgccagc ttgctgacgc cttcagccac
    30001 ttgagacagc acctggctcc accacatgaa gccgaagtca cgggtcagca gccaccgatg
    30061 ggtatcggac atattagcag tataaacgtt tgcggcagga ggggaatcag caaggctgaa
    30121 aggcgagagg tgtcccacgc attctgatcc tttcgctggc gacggggtga tcgccttggt
    30181 tcattgcatg tcctagaagg gaacgacggg ggatactgaa cgtcacacat gctcacgcat
    30241 gcgccaccgg caacgcgcat gttctacgca cgcatgcgac agccaccgct gccgtgagca
    30301 gcaacaccag gccgagtgcc accaggctcg cggcggggcc tacggcatcg gccacccacc
    30361 cgaagcccgt catccccgcc atcgccgacg ccatggagcc gacgctgaag gtggtcacga
    30421 cgcgaccgat cagatgttcc ggcgttaact cctgcaagag tgcccagatg atcggattca
    30481 gcaccgccgt gctgcctccc acgatgatca cgattccggc cgcaatcagc ggcgtttcca
    30541 gcagactcaa actgcatacc gccaggccgc cgatcgtcat cccgctcacg acgatgcgca
    30601 agcgcccctg gatatcactc tcttgcttca acgccagcca ggccgaggcc gccagcatgc
    30661 cgacacccag ggcggaccac aaccaaccca actgtaccgg gccgacctga agaaactcct
    30721 tggcgtaaac cggcaaaata aacacaaagg cgctcacgcc gagattgtag agcgatgaaa
    30781 taatcactag cgtgaggacc atcgactgct ggccgaagat gaatcgaaat ccgacctgga
    30841 gttcctgcca gacggacgta gcctgctcgc tggacacggc atgcgaagga gagaaacgga
    30901 tcggcatgag gcacagggcc gatatcagga atgtggcgga attcacgaac agcacgttct
    30961 ctgccctgat gagggcgatc ataatgccgc tgatcgccgg gccgagcagc atcccgatat
    31021 tattggtgcc ctgaataaga gcattggccg acatgagttc agaaggccga accaataacg
    31081 gaaccgccga cacgagagcg ggaccgaata tggtggacac aaccgacgtg agaaaaataa
    31141 tgccgtacag tcctgtaatc gacagcatgt ccagcgcata gagcgtcgga atgagcagtg
    31201 tgagcagcgc gcgaagaata tcgacccaga tcatgacagg ctttttgggc atgcgatcca
    31261 gatagacgcc gactagcgga cccagcacga gcggaggaat cgtctgcaac agaccgacca
    31321 tggtcatctt catggccgat ccggtcagct catacacgaa ccacaacagc gcaaccttgt
    31381 tgagcccctc accgatctga gacgtcgttt gtccccacca cagcaaccca aaatccctgg
    31441 tgcagataag acgccacccg cgactcgagt ccggagtcga atgatcatct gacatgacga
    31501 cgaatcctcc tggagcaatc gaaggcggga gtggaaatgt tcggtgccct ctcgcaacgt
    31561 ctggagctat gctagccatt tcaaccagac aaccggccta ggaaaggccc tggaggccga
    31621 tcgacaaatc ccgggcagcc cctccacatt tgtcaaaact ggcttcagaa cgcacacggg
    31681 aatattgccc tacccctctg gaatttcttc ctacctgtcg cggcgtcgtc cccgtcgctg
    31741 aagcaacctc ctctcatgtg cgcccgcaga cccttgcgct ttcgtaggct tatggcgaca
    31801 ccacaccact cgatgatccc ggcacaattc ctgcgttact gatctgccat gcctagctca
    31861 tgctccatcc tccttatcga cgacagcccc ggcgaatgcg aattgtttcg ccaggccctt
    31921 acgcaaaccg gctacaaggg gcggctggat attgtggaag ggggccaggc ggccatggcc
    31981 tacctgcatg aacatgtgca gcacgaggag ccggccttga ttctgctcga cctcaagctc
    32041 agaggagaac gaggcgtgga tgtgttgaaa cgcatcaagc aggaggcgcg cgtcgcacac
    32101 attcctgtcg tcatcctgac tagctcagac gacacggcgg atgttcgggc ctgctacagc
    32161 gggggcgcca atgggtacgt cgtcaagccg ggacaattcg atgacctcgt gaccctttcc
    32221 ctgcatctct ggaaattttg gctggaacat aactgcaccc aacggatggc tgccccatgc
    32281 tgacccgtgc cgcgctgaaa aatccgtatg ccgtcttcgc cttgtgcatg atcgcgctga
    32341 ttctcggcgg cgtgtcgtac cagaagatgc gtgtggacat cttccccgaa atcaagctgc
    32401 cctccatcct tgtcaccacg ttctatcgcg gcttgagtcc cagcgaaatg gaaggcgcga
    32461 tcacgctgaa gatggagcaa cgcttcgtcg aagcgagcta cgtcgaacat atcgaatccc
    32521 aatcgctcgc ggggatgagc tacatcaaag tcttttttca accggactat ggcatcgacg
    32581 cggcacaatc cgaactgacc agcctggcct atagcatcat ccgcctgctt ccacccggcg
    32641 tgtatccacc ctctgtttat aaattcggtg tctccagttt gccggtgggc tacctgtcta
    32701 tttccagcga tgcgctcggt ccgaaagaaa ttcgcgatct cgcctacttc accgtgcgcc
    32761 agcagattgc caatatcccc ggcatttcct tcggtccgcc cttgggcggc aaggtccggc
    32821 aaattaccgt cttcctggac caacagcggt tgcttgcgcg cggtatctcc ccctccgatg
    32881 tcgtaaacgc gctcaaccag cagagcgcca tcattcccgc aggcgacatc aaaatcggcg
    32941 acctggacta ttacgtctat tccaatagcc ttgtggacgt cgtggacaag atcaatgacc
    33001 taccgatcaa gatcgtcaac ggcaccccgg ttctgatccg cgatatcggc acggccgccg
    33061 acagcgccgc cgtacagacc tcgatcgtgc gcgtgaacgg ccgcgaagcc acctacattc
    33121 ccatcaccag gcaggaaggc gcgaatacgc tggaagtcac cgatggcatc cgcgccaaac
    33181 tgtccaaact caccgaaatt cccgcaggca ctactgtgaa gtttctctac gatcaatcgc
    33241 tctatatccg gcagtcgatt gccaatcttc agaaggaagg cttgctgggc gctggtctgg
    33301 ccgggttgat gatctttatt ttcctcgcca gcgtgaaagc ggcgctggtg gtcggattgg
    33361 ccatccccct ctcgctgacg gtggcgctgg ttgccctgta tctgaccggc caaagcgtca
    33421 acatcatgac gctcggcgga ttggccctcg tcatcggcac cctgctcgac aacaacatcg
    33481 tcgtgcagga aaacctgcat cgacacttgg aaatgggcaa ggacggacgc tcggcagccg
    33541 aagacagcgc gatggagctg acgctgccta ttctcgtcgc cacgatctgc atcctcatcg
    33601 tgtatctgcc catcatgttt tttaccggca tcatcaagta cctcttcgtg c
//
LOCUS       DAOQDS010000044        33186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_454_length_33186_cov_18.576590, whole genome shotgun sequence.
ACCESSION   DAOQDS010000044 DAOQDS010000000
VERSION     DAOQDS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 33186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33186
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_454_length_33186_cov_18.576590"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PK589_12000"
     CDS             <1..929
                     /locus_tag="PK589_12000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNJ20373.1"
                     /translation="DVTAATATGTLAVDLIDTGGIGGNAPLYLVTRDQATNQVIESRL
                     LFPARATFASTTPLGTPLVVASATVPTTTPVADGTLYYHQDGLGTVTELTDINGAVAK
                     AYAYDAYGNILESPGTVDQPYTYTGREYDSESGLYYYRARYYDPGTGRLLQKDPIGSF
                     LRDVNLYAYVTANPVNGVDPLGLFNPVKGASALLNASIAGVSAASGAAKILIAAGISP
                     AATTGVGALPPTALYLWGTWNVKSAKAAAERALQQWQEAICEDWSQAAWKNLYGALPG
                     GTHYDDPDEANGPWDYIKSQGWWRFFSDLGYF"
     gene            1142..2539
                     /locus_tag="PK589_12005"
     CDS             1142..2539
                     /locus_tag="PK589_12005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20374.1"
                     /translation="MTTLAYSGTKVQSITNPAGRVTQLEYDAAGNLQRLRGADTTAVT
                     FAYDAQHRLIRRTDARNQVTQYTYDASGRFAQATSPDGATRAVIPSHTIAVPNFVAGQ
                     GTADNPAPLSATSVNRATFTDATNHSTTFELDALSRITKQTDALNRVTTITRDARGNP
                     TQIVRPNGAVTTMTYDAKGNLLTSTEQAIAATTTFTYEPTFNQVTSIRDPKGTLTQIA
                     YDAKGNPLTITDALNQVTTFTYNPQGLLLTTKDALNQTTTFTYDALGRLLTTTDPLNR
                     TTTLTYDAAGNVATSTDALTRVTTFVYDVKNRLTQVTDPNTGVTAYAYDGTGNLLTVT
                     DAKNQVTTFAYDGRNRLLSTTDPLGKIERYTYDGNDNLLTRITPKNETISFAYDAVNQ
                     LLSKTLPGSQVTSYLYDQVGNLTNVSDRDSVLTMTREIWRTASSRASRCRTGRRRPTA
                     TIGPVRWLVSCINAE"
     gene            2622..2783
                     /locus_tag="PK589_12010"
     CDS             2622..2783
                     /locus_tag="PK589_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20375.1"
                     /translation="MSVAYRQYGYRLDEIAMHRGVHYATISRCMKQAEQETNDSNDPA
                     PPGMCDCKT"
     gene            2811..3332
                     /locus_tag="PK589_12015"
     CDS             2811..3332
                     /locus_tag="PK589_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20376.1"
                     /translation="MTIKLPAIIPAIVLMSVVLSGCPLPRSEMYKGFSEAEKSYFATA
                     DLGVFPNDARKDVERYQASLVAWPGFITELNSIQKDSAYFTRIVVEHHYYDWILDHSI
                     QKEKYFLSPRGEGVFTTLCPSKLKDENVTKDLNNMLIVYGKPEKVEDGMLHVTCDYAA
                     FIKREWVRTDIFD"
     gene            complement(3799..4662)
                     /locus_tag="PK589_12020"
     CDS             complement(3799..4662)
                     /locus_tag="PK589_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ20377.1"
                     /translation="MNTLLAVDGSDHAYEAVRALKYLRRADQLTLLHVVDAPRPAYPM
                     MVPEVAQELYAQLERSMKEDGEELLTRIQSLLPLHSGPVTKLLQVGSPAEHIVAAAQS
                     CQADLIVMGARGLGPVKERLLGSVSHRVLSMAPCAKFVLHGSLRQLKHILLPLQGPSD
                     AEAALRFLAKQPFQEPVNVSVLTVLPPTRPPWPIDDTAAEKLEAHALRHARDFVEDVA
                     ATLRTQGYTTRSASVLGSPLTMILHEAEKLGADLIMVGSRARQGITRFVLGSVSHAVL
                     HRAPCQVLVFE"
     gene            complement(4659..5333)
                     /locus_tag="PK589_12025"
     CDS             complement(4659..5333)
                     /locus_tag="PK589_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNJ20378.1"
                     /translation="MRMESISQITITGDGIALDGILGLPGQPKGVIAFAHGSGSGRFS
                     PRNQFVARVLETGGFATLLLDLLSPDEADDRRKIFDIDLLADRLLLAQHWLQTHASTR
                     TLRTGYFGASTGAGAALQAAAREPDAVGAVVSRGGRPDLAGPYLSRVIAPTLLLVGGE
                     DGPVIGMNEEALGQLTCQKRLIIIPGATHLFEEPGTLEQVAQEALNWFRRYLHPAPYQ
                     PKELLP"
     gene            5809..6312
                     /locus_tag="PK589_12030"
     CDS             5809..6312
                     /locus_tag="PK589_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ20379.1"
                     /translation="MVERLFTKILVPVDFSPCSEEAYRIALSFARSYQAEVLLLHVVD
                     TKSLDALNRLGLAPASEAAKQKKQLHHHARLNARQLLAWDEAKGLSIKRVLADGCPFE
                     EIARTARVEGVDLVVMGSYGGAVGGVEKIFFGTTAEKVVRTAGCPVLTVPLPVKRAKA
                     RSGKSSS"
     gene            6325..6753
                     /locus_tag="PK589_12035"
     CDS             6325..6753
                     /locus_tag="PK589_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNJ20380.1"
                     /translation="MGLKQRSAVLSGGRVWLAICFVLALCSQGGAQAEQRIDVAIKDF
                     AFVTKQVPLRLGVATVITITNEDQERHDFGSTMFDGLPTRVESGGIVSYGRGISGVFL
                     DGKKSAVIRFVMERPGRHEFRCSIHQNMKGELLLLNVEAA"
     gene            6757..7695
                     /locus_tag="PK589_12040"
     CDS             6757..7695
                     /locus_tag="PK589_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ20381.1"
                     /translation="MVPLIKHMLFATDFSACADRAMGYALSLAGAWKANLCVVTVLEL
                     YPGMDPDYTVNKMYLDHLREEANRQLGAVQARAKAASQSVTTRIEVGIPSQALQTVAE
                     STGADLLVVGTHGRTGLDHVLVGSTAERVVRVSPCPVLAVKADKGVADSSAAAPIKRI
                     VVPVDLSTCSLDALEYAVQFAKPLGAAITILHAMEPVAYGLDFSLSHAKEWKEQRDYL
                     EKRLSVLSACVTAHGIQADHVLKPGLPADSIVSHVTQQGFDLMIMGTHGRRGISHVLL
                     GSIAGAMLRHAPCPVLTVLQVNFGADYERVVPLGNS"
     gene            7726..7950
                     /locus_tag="PK589_12045"
     CDS             7726..7950
                     /locus_tag="PK589_12045"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNJ20382.1"
                     /translation="MKRKEQSRAAAPPSPRAKEQTAVNPSALPDGMWERISQKAYDLW
                     RERGSREGFALEDWLDAEAIVMEEMHESRE"
     gene            7934..9289
                     /locus_tag="PK589_12050"
     CDS             7934..9289
                     /locus_tag="PK589_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20383.1"
                     /translation="MNLANERVVGRSAPVNKDLGRLISRLDQLSLSPGFAQQRRIAFE
                     RALQVYVELGSQSPLTPLREEVELADLLLYADFYPEDGQLTLIEQLRDVITEHIPEEE
                     RVWLDPLKHSYLDLVEILPSQAGAARRSLKSLGDGRLYDIPAESVPRDAALGSVLFTR
                     LVREPGDPESRLAVLAGPMLVLSIQDARALYETTGEERREMEVVGGSFELGDWPEFAK
                     RFGHLLLKNFARMRLAALLEAVSDIRYRTETDAPYFYVMALYEHHEFTFISGGMTELE
                     GWAEDAPPEQAVTGSRRKLRRFVRRGQKELVARITVTATELFLECDSRGSLDEIKHTL
                     AATFGYSLHFRGEIAQPPVRQVTQEELSGNEPLTVVVTGEEDRSLVKGFLETVYLEWA
                     DRESPALGGDTPRHTAKTPEGRDRVAALIDDMERCDFGLVRSGIAAFDYNVLRAHVGL
                     C"
     gene            9308..9799
                     /locus_tag="PK589_12055"
     CDS             9308..9799
                     /locus_tag="PK589_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNJ20384.1"
                     /translation="MAQDRRTMMDGGEGMQEQVERIVLQARAEPPNVLKTLLAIEEHL
                     GHVPAEAIPVIARRLGATTAQIAGVLSYYPDLRVTAPGRHVIRVCMGESCYANGCGRV
                     LRELQDRLRTDVGETTSGGKFTLDTMSCAGNCAVSPTVVIDRDLYGRVLPSQVETMLE
                     RYR"
     gene            9801..11312
                     /locus_tag="PK589_12060"
     CDS             9801..11312
                     /locus_tag="PK589_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNJ20385.1"
                     /translation="MTSATRLYLSNDTSARAAGAGVLADAWAERPEVQLIRISSRGAF
                     FLEPMVERDSPEGRIAWFNVQPDDLASILSCNGGTPVRAIPFLANQTRFTFANFGETE
                     PLALDEYQARGGLKGLEESFRLTPDAIIEELRQSQLRGRGGAAFPVWNKWKVAQQTRA
                     SEKYVVANADEGDAGTYCDRMILEGDPFRLLEGMLICARAIGASRGYVYCRQEYPAAA
                     ATLRAAIHKADEAELLELRGEPFPIEVVEGAGSYVCGEETALLESLEGRRGVVRARPP
                     YPAQAGLYGHPTIVSNVLTFATIPQILSRGGAWHASLGTEQSRGTIALQLGGRVKHPG
                     LVEVPFGLSLRQVLDQFGGGMAPGSRFKAAQVGGPLGSLFPAAGLDLPICYDAFAKAG
                     AVLGHGGIVVYDHETDMVDLARHFMAFTAQESCGKCTPCRIGSVRGREILERIQAGGG
                     TVEDLALLDDLGETMKLTSLCALGGRAPYPVLTAIEHFPVEFRNKLSAVRRQS"
     gene            11342..14065
                     /gene="fdhF"
                     /locus_tag="PK589_12065"
     CDS             11342..14065
                     /gene="fdhF"
                     /locus_tag="PK589_12065"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNJ20386.1"
                     /translation="MKLSINGRVIVASPGDSIYRAAKQAGIGIPGLCTSDHLNPFGSC
                     RLCLCEVEGQSGTPASCTTPVREGMVVHTESERLTRLRKHLLELYLSEQPAGDRVPEP
                     LQALARSLGLTHVRYLQPAGRVPVVDRSNPFFVFDNAVCISCARCVRACDEIQGTHAL
                     TMLYRGFSSRPAAGAAALSGEAAGFASSNCVSCGSCVKECPTGALIEKTVLEEGEPVQ
                     AVRTTCAYCGVGCTFDAGVREGRVVRMGPADDGPSNHGHACMKGRFGWTYNYAPDRLR
                     TPLLRQGDKWVELSWPAALDRIAEEWSRIKEAHGPDALATISSSRGTNEENYLFGKFM
                     RCVIGSNHIDNCARVCHSATVTGMMETLGASAATNSIHDLDLAKLILVVGANPTESHP
                     VLGARIKQAARRGVPLIVIDPRRTELARLADLHLQLHPGTNVALLNGMGHVIAKEHLT
                     DAAFVRDRTEGFEEWLNVVEGCTPELTSRLTGVPAHLITEAARRYATSGGSMAVHGLG
                     MTEHRWGSHGVMALVNLALATGNIGKPGTGINPLRGQNNVQGASDVGCLPTFFAGYQP
                     LTDPALAAAHLAVTGRPLPTARGMKTPDMWDAALAGTLKSLWIIGYDVAQTDPNLKKV
                     HAALKQLDFLIVQDLFFSETAKLAHLVIPGASFLEKDGTFTNLERRIQRIRKAVEPPD
                     GVLPDWQVVCEVSARMGYPMQYSHPSAIMDEIAQLAPLFAGVSFDRLDAPEGLQWPVP
                     SKDHPGTSLMHEQSFPKGKAKFVAVDYLPPGETPSEAYPLVLITGRILQHYNCGAQTR
                     RTEIMQVVDTDVLEMHALDATRLDVRDGDPVRLISARGEARLPVMISDRVQPGELFTS
                     FHFPDSDLNVLLSSSADESSKCPEYKVSTVRVEKVLSTGTPTPSMHVMLIT"
     gene            14062..14907
                     /locus_tag="PK589_12070"
     CDS             14062..14907
                     /locus_tag="PK589_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNJ20387.1"
                     /translation="MTAEPVPSATGSVADAYPPPEIAARVCKLGLAKVNTAVPTMVAL
                     AVLAGAFISLGALFYTVTITAGQDGSPLPFGLLRLTGGVTFSLGLILVVVGGAELFTG
                     NNLIAMAWAAGCVQTRQVVRNWIWVYLGNLLGACGTAGLVLLAGIQLLGDGAVGEAMV
                     RIARSKIALDPLSAFARGILCNVLVCLAVWLCMGARSVADKILAIVFPISAFVACGFE
                     HSVANMFFLPLGIALAAGSAAPLSLIDALSNLALVTLGNIIGGTVLVALVYWFIYLRT
                     GSQPR"
     gene            complement(14972..15376)
                     /locus_tag="PK589_12075"
     CDS             complement(14972..15376)
                     /locus_tag="PK589_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20388.1"
                     /translation="MNVTNLGFTWNSRYLELSPTEMAIALVAALLVGGAALYFLLKVL
                     RSKPVKVLGMALLVAGIALVGWLALDQHQQDERARVSEIWSRTFENSAIKDFESCVAN
                     RTPRSPGPQYRVEVVTECGDVAKAIKEGLEAR"
     gene            15602..16867
                     /locus_tag="PK589_12080"
     CDS             15602..16867
                     /locus_tag="PK589_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ20389.1"
                     /translation="MSHSSIRSYLTPRLGIMLPLGFASGLPLALTGGTLQAWLTEAGL
                     DLTTIGLFAYVGLPYTLKFLWAPVMDRIVPPWLGRRRGWMLVTQSGLALALTVMALIG
                     PAEGAQVFAALALAVAFLSASQDIVFDAYRTDLLKPEERGLGAATWVMGYRIAMIASG
                     SLALILAARLNWSAAYLCMSALMALGIVTILLSPEPEDAQVAPKSMAEAVWGPLSEFL
                     SRPMALALLGLIVLYKLGDAFAGALTTSFLLRGMQFSSEDIGVVRAFGMGATILGAFI
                     GGGLMPRLGLFRSLFVFGVLQALSNLSFMWLAWAGKSYAVMAFAVGAENLTGGMGTAA
                     FVALVMSLCNHRYTATQFALLSSVEALGRVFLGWPAAKLVGLAGWGPFFFVTFLAALP
                     GLWLLWVLRKPVAQHAELNAGRSAAGAPC"
     gene            complement(16908..17198)
                     /locus_tag="PK589_12085"
     CDS             complement(16908..17198)
                     /locus_tag="PK589_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20390.1"
                     /translation="MFVWSKRLVTGLLAGFVLLIGIFLLQGEPETISGLKIHTTRWMA
                     VNYIGLLVWLFVWMFDQARVRGKNVFFWLVPFALAPLPTLMLFILFLQRKMK"
     gene            17355..17726
                     /locus_tag="PK589_12090"
     CDS             17355..17726
                     /locus_tag="PK589_12090"
                     /inference="COORDINATES: protein motif:HMM:NF025629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4359 domain-containing protein"
                     /protein_id="HNJ20391.1"
                     /translation="MRTGTLVGLVGSLMLAVWLAATNPTMDTYLQFVEARLSAEIDKM
                     DQSGPRRDRDLIRSIFRAQGPKLVEGVVRPNTRRNNWGLLSLFETNVLDQPVLVLGIG
                     GQFVPLRGVEEATVKVGRLAF"
     gene            18055..21597
                     /locus_tag="PK589_12095"
     CDS             18055..21597
                     /locus_tag="PK589_12095"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNJ20392.1"
                     /translation="MRIERRFTKRGQSPYEGLAFVKRSSEIRNPDGSTVFKLEHIDIP
                     EHWTQLAIDILAQKYFRKAGVPQVHEDGTPVVDAAGKPVLGGERDSRQVFNRLAGCWT
                     FWGKNHGYFKTPEDATAFHDEMCYMLAYQMAAPNSPQWFNTGLHYAYGLSGPAQGHFY
                     VDPKLGEVVRATNAFEHPQPHACFIQSIEDDLVNENGIMDLWVREARLFKYGSGTGTN
                     FSRLRGDGESLSGGGRSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVCLDLDHPDIEE
                     FIDWKVVEEQKVAAMVTGSKICAQRLNAVLKACQSVDAQGQAHLELDMKQNQALREAV
                     TAARRDMVPEAYIHRMFDYAKQGYTHFVFHEYDTNWDGKAYQTVSGQNSNNSVRIPNG
                     FFDALEHDGDWELRRRIDGKISKTIKARDLWDKIAWAAWICADPGTQYDTTINEWHTC
                     PEDGRINASNPCSEYMFLDDTACNLASLNLAKFFNAEGEFDLDSFRHAVRLWTVALEI
                     SVLMASFPSRAIAQKSYDYRTLGLGYANLGTILMKQSIPYDSQKATAICGAITAIMTG
                     ESYATSAEMAAEIGPFPGYNRNRESMLRVIRNHRRAAYSAPPEEYEGLTILPASIQPE
                     HCPPAMLLAARRAWDRALELGTAYGFRNAQVTVIAPTGTIGLVMDCDTTGIEPDFALV
                     KFKKLAGGGYFKIINQSLPPALQALGYTEAQIRDIVAYCAGHQTLKGAPFINHEVLRQ
                     KGFDEAALERLEGSLAQAFEIQFVFNKYTLGEEFCRQKLGISEAQLADPAFNMLKTLG
                     FTQEDVAAANDYCCGTMTVEGAPHLKLEHLPIFDCANRCGRIGQRYIAVDAHIRMMAA
                     AQPFISGAISKTINMPADATLEDVKAAYLFAWKSMVKAVALYRDGSKLSQPLNASSDS
                     GKSAEAAPSVATVAEKVAERVLVRYLHKRRSLPARRGGYTQKAIIGGHKLYLRTGEYE
                     DGTLGEIFLDMHKEGAAFRSLMNNFAIAISLGLQHGVPLEEFVEAFVFTRFEPNGPVK
                     LNDRIKMATSIIDYIFRELAITYLERKDLSQVQEDDLRMDSMKKDEQDPECVEEEVDM
                     TALKKASILTEHLPVRRNGMNGGNGHTQRAGNGSVAHKLELKRETLTVTSVRQEAKEK
                     GYEGDPCQNCKQFTLVRNGTCLKCMSCGETSGCS"
     gene            21852..22217
                     /locus_tag="PK589_12100"
     CDS             21852..22217
                     /locus_tag="PK589_12100"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ20393.1"
                     /translation="MKKRYSSRASVQSTCVLSCNGLVGQAEMVNLSVPGCLLTTRMKL
                     KVGQYVDLRLTFHTSPAPMQIKLAAVRWVSDGQVGLEFIRMSEADQTRLRWLAGHVEQ
                     RKPQANWSEPVVCLSAAYT"
     gene            22597..22827
                     /locus_tag="PK589_12105"
     CDS             22597..22827
                     /locus_tag="PK589_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20394.1"
                     /translation="MMLRSIRKEVLEFAKVTERLLGAASLTHELTEEECTLIALYAKD
                     LSADQQPWSKFIPGTQHQDPAYPSKGMWESSA"
     gene            23510..25084
                     /locus_tag="PK589_12110"
     CDS             23510..25084
                     /locus_tag="PK589_12110"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNJ20395.1"
                     /translation="MFTPPHISRWMKWVGGIVVVLVTAMLLVAVIADEPARKYVEDKA
                     NASLSVYTLRIGKLDLHPLTLSLDLENVTLVQKRHPDPPMVHIPKWHAGIQLWGLLTG
                     KVVSDHRIDGPSATVTRPQAKTELKETRPSGWQDIVRNLLPLRVSDVKVKQAQVTYYD
                     HPKAQPVKLTDLQFEAREISNRGGEGQEYPSTIKMEARLPQGGRITAEGHANALAKPL
                     PAFNVDFSLDDIALRDFVGLAGGYNVQLDRGKITANGHAEYAPWKQVADVREIRLEGV
                     KADYVYRRHPRDEARREEAVAVAKKAKDNPLLVINVKQGKILDSEFGFVNQSVTPHYR
                     VFVGEVNADLDNFTTRLRELQGGDAVIKVTGRFMGTGRSVIVGTFRPEKPSPDFDVGV
                     QIVKTELKSFNDVLRAYGDFDVHRGNLSVFSELSIRHNQVSGYLKPIFKDVEAYDPNQ
                     DADKAWTKKVFESVIDGIVKLLNNTSEDQVAADAGISGPVKNPSADTWQVMGTLIQNA
                     FFKAILPGLEKEHGKA"
     gene            25131..26222
                     /locus_tag="PK589_12115"
     CDS             25131..26222
                     /locus_tag="PK589_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20396.1"
                     /translation="MIAAKDVVKSARTAGLRYVREGMGGFRRVARRGTSVYLNSSGVR
                     LRDERQLHRIRSLVIPPAWTEVWICGDPNGHLQATGRDARGRKQYRYHQRWREVRDEN
                     KYGRMLMFARLLPRIRQRIERDLALPGLPREKILATVVRLLETTLIRVGNEEYAKTNN
                     SFGLTTMRDRHARVEGARVRFEFRGKSGVQHLVDLTDRRLARIVRQSQDLPGYELFQY
                     VDAEGARHGIDAGDVNDYLRHIAGEECTAKDFRTWAGTVLAAKALQECRAIGSAAQAK
                     RNIVAAIEVVAKRLGNTKTICRKCYVHPEIVQAYLEGSLARSLTTRIRRPFARMAGCL
                     TPDEAAVLACLERRLGRKASRGIGLRSSA"
     gene            complement(26570..27070)
                     /locus_tag="PK589_12120"
     CDS             complement(26570..27070)
                     /locus_tag="PK589_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNJ20397.1"
                     /translation="MLWLFTTHGSVGMFIVRVTLAIVIFAHGAQKLLGWFGGQGFTGT
                     IGFFTTSMGLPWLIAFLVIIGESIGSLSLLAGFLTRFVAASYLLIMLGAVAMVHWPNG
                     FFMNWFGQQQGEGFEYHLLIIGMSVALLLEGGGAWSVDHLIARWMAQHGWAGTQPLHA
                     FAQSKQ"
     gene            complement(27307..27621)
                     /locus_tag="PK589_12125"
     CDS             complement(27307..27621)
                     /locus_tag="PK589_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20398.1"
                     /translation="MMDTVHTISNEQPTYEESVRLGWALLWRGVGSFVLLMFAGNIGL
                     LFLIPELTRTVPTLWIALLPLAFATLISAFLIMPFVVRRLVKAPFKGFHLSFVRDRLL
                     GQ"
     gene            28026..>28268
                     /locus_tag="PK589_12130"
     CDS             28026..>28268
                     /locus_tag="PK589_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ20399.1"
                     /translation="MLRILIGDDHAVFRRGTKDVLQEHFAPLEVHEAKTGWEMVTLAS
                     QGKWDAFIMDVTMPGKSGIDLLEHIRSLHPSTPVLVY"
     assembly_gap    28269..28368
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28369..28810
                     /locus_tag="PK589_12135"
     CDS             <28369..28810
                     /locus_tag="PK589_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ20400.1"
                     /translation="DLLEHIRSLHPSTPVLVYSMHSEESYAVRMIRSGASGYLNKSTG
                     LDELVAAMRSILAGQEFVNPAVARCLIERMRTKDSGQLHDALSNREFQILRMVAQGES
                     LKQIALTLCVSANTVSTYRTRILQKLRLKTNAELTRYALEHALI"
     gene            complement(29044..29346)
                     /locus_tag="PK589_12140"
     CDS             complement(29044..29346)
                     /locus_tag="PK589_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNJ20401.1"
                     /translation="MHTPCDLSLLIDGLSSTVTRADIVALFSSCGKVTAAEIVIDADG
                     MPLGVARVTMSTKEEARFAIRTFHRCRFRGHTLLVFEDTTKNQREDLWNSRMFKRP"
     gene            complement(29730..30995)
                     /locus_tag="PK589_12145"
     CDS             complement(29730..30995)
                     /locus_tag="PK589_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ20402.1"
                     /translation="MPTSGAQIERLRRKAEAYLRESAHTPPIDQSATETAQLVHELRV
                     HHVALRLQCEELRQTHLELEESRNRYVELYESIPVGYFSLNPRGAICDVNPAGAAMLH
                     QPMNRLIGRRFHLFMPVAQRRRFIEFCKQVCFERKRLSCEVDLGDASDDQPRALMNVL
                     LEGSPAQGQKDIMRLAVIDITRRKEAEQQLKVQEDELRTSRQKLQDLNAQLLNIQDEE
                     RRAIARELHDNCCQQLAMMMFSVNSIERCCDEPLTHKVRMLNEQIKRTLESVRHIAYG
                     LHPAMWDTMGVEEAMRSYLKDFIEVTELPVDFHAIDVPRRVPEVISTCLFRTLQEMLH
                     NVVKYAQASRVEVRLIKNENTLSLYVTDHGRGFHVKTMKQSPRGLGLVSLKERAQLLN
                     GTLNVTSQIGVGTMVHVILPLTSGNDHPM"
     gene            31357..32361
                     /locus_tag="PK589_12150"
     CDS             31357..32361
                     /locus_tag="PK589_12150"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNJ20403.1"
                     /translation="MPATLVLERWAANWKMGSDVVPGAARLLWNATKQHAILGQILIM
                     TCGLAAHMEEALITPNVEHEPCWQPWLWLAREYGYRACWSFPVRTFGGPILGTLAWYF
                     KDPREPSLRMMELAATLTHAAAIIISRHREAEERTRVEAALRESDARYRAFVAVTSDI
                     VYKMSADWSEMQSLVGKEFIASTERPRRDWMDAYIPDADRTVVWEAIGAAIHSKQAFE
                     LEHQVIRLDGTIGWTYSRAVPLFDQKGEIVEWFGTASDITERKQAEAQLKTRNIELER
                     FNKVMVGRELRMMELNHKSPGRQGLQSESPLDQFTIEAQRPAAEPGQGDPGGESPRRA
                     "
     gene            32456..32749
                     /locus_tag="PK589_12155"
     CDS             32456..32749
                     /locus_tag="PK589_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNJ20404.1"
                     /translation="MRMTVDQQVVAREWLARGFSCDLWVDPPGQVWEDYRHATDELVM
                     PVAGRLELEFEGQCFRPAPGEEIFIPACVSHTVRNIGGTTAKWIYGYKRVHTG"
     gene            32780..>33186
                     /locus_tag="PK589_12160"
     CDS             32780..>33186
                     /locus_tag="PK589_12160"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20405.1"
                     /translation="MPTTTQFVVSGQSKPGVLASVAAVLGAAGVNIKAFSAPEVTGPG
                     KLRLIVADVDAARIALRKAKVKFREETALILSLENKPGALKQVADSLTRARINVKCGY
                     CTPSREGKRAIVVLTVSNTTKALGVLRTHSLDE"
BASE COUNT         6882 a         9269 c        10109 g         6826 t
ORIGIN      
        1 tcgatgtcac ggccgccacg gccaccggca ccctcgccgt ggacctgatc gatacagggg
       61 gcatcggggg caatgctccg ctctatctgg tgacgcgcga ccaggccacg aaccaggtga
      121 tcgaaagccg cctgctgttt cccgcccgtg ccacgtttgc ctcgacgacg cccctgggga
      181 cgccgctcgt ggtggcctcg gcgacggtgc ccacgaccac gccggtggcg gatggcacgc
      241 tgtactacca tcaggacggc ctcggtacgg tgacggagct gaccgatatc aatggcgcgg
      301 tggccaaggc ctatgcgtac gatgcgtacg ggaatatcct ggaatcaccg ggcacggtgg
      361 atcagccgta tacgtatacg gggagggaat acgattcaga aagcggactc tattattacc
      421 gggcgcggta ctatgaccca ggaacgggac ggcttcttca gaaggatccg ataggatcat
      481 tccttaggga cgtaaatctg tatgcttacg ttaccgcaaa cccagtcaac ggcgttgatc
      541 cattagggtt attcaatccc gtaaagggag caagtgcact tttgaatgct agcattgcag
      601 gagtatcggc ggcctctgga gctgcgaaga tcttgattgc tgctggtatt tcacctgcgg
      661 caactacggg agtcggtgcg ttgccaccga cggctctcta cctttggggg acatggaacg
      721 ttaagtctgc taaagcagca gcagaaagag cccttcagca atggcaggaa gcaatctgtg
      781 aagattggtc acaggcagct tggaaaaatc tgtatggtgc gcttcctggt ggaacccatt
      841 atgacgatcc ggatgaggct aatggtccgt gggattacat caagagtcaa ggttggtgga
      901 gattcttttc tgacctgggg tatttttgat tcttctcagc aacaatgggt aactcatgac
      961 agatgttttt attgccatct ttgagatagc ggcggtgctt gctatctctc tttatgctac
     1021 gtggctcctg gtctctagac taaaacgagg agaatcgaag ccgctaagct tcagagagtg
     1081 gattaagcac atattggagg ccatttgggg attatagaac cacacggacc cgaccgggca
     1141 ggtgaccacc ctggcctata gtgggaccaa ggtgcagagc atcacgaacc cggcgggccg
     1201 cgtgacgcaa ttggagtatg atgcggcggg caacttgcag cggcttcgcg gcgctgatac
     1261 gacagcggtg acgttcgctt atgatgcaca gcatcggctg attcgtcgga cagacgccag
     1321 aaatcaggtc acgcagtaca cctatgatgc cagcggtcga tttgcccagg cgacctcgcc
     1381 ggacggggcg acccgcgcgg tcatccccag tcacaccatc gcggtgccca atttcgtcgc
     1441 agggcagggg acggccgaca acccggcgcc cttgagtgcc acgagtgtca accgggcgac
     1501 gtttacggat gccaccaatc acagcacaac ctttgaactg gacgcgctga gccgtatcac
     1561 caagcagacc gatgcgttga accgcgtgac caccatcacg cgggatgccc ggggcaatcc
     1621 cacacagatc gtccgcccca atggcgcggt caccaccatg acctacgacg cgaagggtaa
     1681 tctgctcacg agtacggagc aggccattgc cgcgacgacg acatttacgt acgagccgac
     1741 cttcaaccag gtgaccagca tccgcgaccc caaaggcacc ctcacgcaga ttgcgtatga
     1801 tgccaagggc aatccgctga cgatcaccga cgccctgaac caggtgacca cattcacgta
     1861 caacccgcag ggccttttgc tcaccactaa ggacgccctg aatcagacca ccacgtttac
     1921 ctatgacgcg ctgggccgac tgctcaccac gaccgatcca ctgaaccgga cgaccacgct
     1981 gacctacgat gcggcgggca acgtcgcgac cagcaccgat gccctcaccc gcgtcaccac
     2041 gttcgtctat gacgtgaaga accggctcac gcaggtgacc gatcccaata cgggagtcac
     2101 ggcctatgcc tacgacggca ccggcaatct cctaaccgtg accgacgcca agaatcaggt
     2161 gaccaccttt gcctacgatg gccgaaaccg gctgctcagc accaccgacc ccctcggcaa
     2221 gatcgagcgc tacacctacg acggcaacga caacctgctc acgagaatca cgcccaagaa
     2281 cgagaccatt agcttcgctt acgatgccgt caatcaattg ctcagcaaga ctctgccggg
     2341 cagccaggtc acgagctacc tctatgacca agtcggcaac ttgaccaatg tgagcgatcg
     2401 agatagcgtg ctgactatga ctcgagagat ctggcgaacc gcctcgtcac gagcctcacg
     2461 ctgtcgaact gggcgcagac gacctacagc gacgatcggg cccgtcaggt ggttagtgtc
     2521 ctgcatcaac gctgaatgag ggttcttagc gacgaggagt ggtaagccac acttcggcca
     2581 aggctttctc taaactccga ccgggcgagg ggacagggct cgtgtcggtg gcgtaccgcc
     2641 agtatggtta tcggttagac gaaattgcca tgcatcgcgg cgtgcactat gcgacgatca
     2701 gtcgctgcat gaagcaagcc gagcaggaaa caaacgatag caacgacccg gctcctccgg
     2761 gcatgtgtga ttgcaagacc tgaccccttt tggattagtt tgagatacca atgacgataa
     2821 agctgcccgc cattattccg gccatcgtac tcatgtccgt ggttctatcc ggctgcccat
     2881 tacccagatc agaaatgtat aagggtttct ccgaggcgga aaagtcatat tttgcaacag
     2941 ccgacctcgg tgtctttccg aatgatgcga gaaaagatgt cgaacgatat caggctagcc
     3001 tggtggcatg gccgggcttc ataacagagc tgaattcgat acagaaagac agcgcatact
     3061 tcacgagaat tgtcgtggag catcactatt acgattggat actggaccat agcattcaaa
     3121 aagaaaaata tttcctttct ccgcgagggg agggggtgtt cacaacactt tgtccgtcca
     3181 agttgaaaga cgagaatgtc actaaggatt taaacaatat gctcatcgtt tatggaaaac
     3241 cggaaaaagt ggaagatggg atgctacacg tgacttgtga ttacgcggca ttcataaaga
     3301 gggaatgggt gagaacagat attttcgact agggtcgtcc cggtgagtct gtaaagtggc
     3361 tcaatacacc agggcctgga gccggaaccg tcctgaggaa atctaagtaa gtgtgggagc
     3421 attcgaaagg atatggaagg acttggtctc ccaatgatga ccaggagagc atatgccagc
     3481 cccagaaggg gttttgcact catacatgtg agttgctcac gtctcgccca tggcccgttc
     3541 gttgcgcata gagtttcccg ctgccgtcta tcatgtgacc agtcgtggca atgccacgca
     3601 ggatattgta gttgaccatc aggatcgcac gagcatcctg agctgctcgc acacatgatt
     3661 caccattgca agacctaacc ctccaactcc ggactcccta agtcgtcgga gagaccctag
     3721 aattaagtcg cacagtacac cgatacgacg gcaacgacga tctcaccaaa cgccgcgcga
     3781 tcgagggcgg cgactctatc actcgaacac cagcacttgg catggggccc ggtggagtac
     3841 ggcgtgagag acgctcccca ggacgaagcg ggtgatgccc tgtctggcgc gtgagccgac
     3901 cataatcaga tctgccccca atttttccgc ctcgtgcaga atcatggtga gcggggaacc
     3961 cagcacactc gcactgcggg ttgtatagcc ctgtgtgcga agggtcgccg ccacatcctc
     4021 cacgaagtcg cgggcgtgac gcagcgcgtg cgcctctaat ttttcagccg cggtatcgtc
     4081 tattggccat gggggacgtg tgggcggaag gacggtcagg acgctcacat tcaccggttc
     4141 ctgaaagggc tgtttggcga gaaaccggag tgccgcctcg gcgtcggacg gtccctgcaa
     4201 cgggaggagg atgtgcttca actggcgcaa tgatccgtga agaacgaatt tggcgcacgg
     4261 agccatactc aatacgcgat gagagacgct gccgagcagc cgttccttca ccgggcccag
     4321 gcctctcgcc cccatgacga tgagatccgc ctggcaggat tgagccgcag ccacgatatg
     4381 ctccgccggt gatcccacct gcagcagttt ggtgaccggc ccgctgtgaa ggggaaggag
     4441 cgactgaatc cgcgtcaaca attcctcgcc gtcctctttc atgctgcgtt ccaactgagc
     4501 atacagttcc tgcgccactt ccggcaccat catagggtaa gccggacgcg gggcatcaac
     4561 cacgtgcaga agtgtgagct gatccgcgcg gcgcagatac ttcagcgccc tcacggcttc
     4621 atacgcatgg tcggatccat cgactgcgag caacgtgttc atggcaggag ctcctttggt
     4681 tggtacggtg ccggatggag ataccgacga aaccagttga gggcctcttg ggcgacctgc
     4741 tcaagtgtgc ccggttcttc gaagagatgc gtggcccccg ggataatgat caatcgtttc
     4801 tgacaagtca gctgcccgag tgcctcctca ttcatgccga tcaccggacc atcctcgcca
     4861 cccacgagca acaaggtcgg cgcgatgacg cggctcaggt acggaccggc gagatcgggg
     4921 cgcccgccgc gcgagacgac ggcacccacc gcatcgggct cgcgcgcggc cgcttggagg
     4981 gcagctccgg ctcctgtgct ggccccgaaa tatcccgtgc gaagagttcg cgtcgaggca
     5041 tgggtctgca gccagtgctg tgctaggagc aagcggtcag ccaggaggtc gatgtcgaaa
     5101 atcttccgcc gatcgtcggc ctcatcgggc gacagcagat ccaggagcag ggtggcgaat
     5161 ccgcctgtct ccaggacgcg ggcgacgaac tgattgcggg ggctgaagcg accgctgccg
     5221 ctgccatggg caaacgcaat gacccccttc ggctgccccg gaagtccgag gattccgtcg
     5281 agggcgattc catccccggt gatcgtgatc tgagaaatgc tctccatcct catacgtgcc
     5341 gaacgacgtc cttcaggcga ggttcaatgg cattccaccg gacagaagaa aatcaggtac
     5401 actgcgatgc ccgtccccag cagcaaggcc ccgatcaacc accaggatgt gcgcgacatg
     5461 gtgcattcct ttccaagact ggagcgagca agagcaatgc catcacggtt tcgcagactg
     5521 cttcgtacag tgagtatggc ggcgtcttag atccccgcct gaagcggcga cgcatgagat
     5581 cggttattct tcgtaaatgc gacgggttcg gcaatctgtg gcgttccgct acagatggtg
     5641 gatggagcct gtagcaagac accacctcac aggtctctgt gtcggggggc ggcccatggc
     5701 ataccgtgat tgcaggggaa tcctgatgat tctgtgctgg tatcggagcc aagctgatgg
     5761 cattgggctt gctgatgtgt aatacagcag aaagggtgga gaggtcggat ggtcgaacgt
     5821 ctgttcacga aaattctcgt tccggtcgat ttctcgccat gctctgagga ggcctatcgg
     5881 atcgcgcttt cctttgcgcg ttcctatcag gcggaggtcc tcctgctcca cgtcgtcgac
     5941 acaaaaagtt tggacgccct caaccggctg ggattggccc ccgcgtcgga agccgcgaag
     6001 caaaaaaagc aactgcatca tcatgctcgg ttgaacgccc gccagttact ggcctgggat
     6061 gaggcgaagg ggctatcgat caagcgggtg ctcgcggacg gctgcccgtt cgaggagatt
     6121 gcaaggaccg cgagggtgga aggggttgat ttagtcgtga tgggcagtta tggaggagca
     6181 gtgggcgggg tcgaaaagat tttcttcggc acaacggcgg aaaaagtggt gcggaccgcc
     6241 ggctgtcctg tcttgacggt acccttgccg gtgaagcggg cgaaagcgcg aagcgggaag
     6301 agttcatcat aaggaggcat ggtcatgggg ctgaagcaga ggtcagcggt tctcagcgga
     6361 gggcgggtct ggttggcaat ctgtttcgtg ctggcgctct gttcccaggg tggcgctcag
     6421 gcggaacagc ggatcgatgt ggcgatcaag gactttgcgt tcgtcaccaa gcaggtgccg
     6481 ctgcggctgg gggtggcgac ggtgatcacc atcacgaatg aggaccagga acgccacgat
     6541 ttcggatcga cgatgttcga tggccttccg acacgggtcg aatccggcgg cattgtctct
     6601 tatggccggg ggatcagcgg agtgttcctg gatggcaaaa aatcggccgt catccggttc
     6661 gtgatggagc ggccgggacg gcacgaattt cgttgttcga ttcatcagaa catgaaaggg
     6721 gaattgctcc tcctgaatgt ggaggcggca taagccatgg tcccactgat caagcacatg
     6781 ctgttcgcca ccgacttctc ggcctgcgcc gatcgggcca tggggtatgc tctgagcctg
     6841 gccggagcct ggaaggctaa tctctgcgtc gtgaccgtgc tggagctcta tccggggatg
     6901 gatccggact atacggtcaa caagatgtac ctggaccact tgcgcgaaga ggcgaaccgc
     6961 caattgggcg cggttcaggc ccgcgcgaag gcggccagtc agtcagtcac gacgcgcatc
     7021 gaagtgggaa tccccagtca agcgcttcaa accgtcgccg agtcgaccgg cgcggatctc
     7081 ctggtcgtcg gcacgcatgg ccggacgggg ctggatcatg tcttggtggg cagcacggcg
     7141 gagcgcgtcg tgcgggtctc tccttgtcct gtcctggcgg tcaaggcaga caagggtgtt
     7201 gccgattcgt ctgcggcggc gccaatcaag cggatcgtgg tgccggtgga tctctccacc
     7261 tgttcgctgg atgccttgga gtatgccgtg cagtttgcca aaccgcttgg ggccgcaatc
     7321 accatcctcc atgcgatgga accggttgcc tacggcctgg atttcagttt gagccacgcc
     7381 aaggagtgga aggagcaacg ggattattta gagaagcggt tgagcgtgtt atctgcctgc
     7441 gtgacggccc acggaatcca ggccgatcat gtgctgaagc cggggctgcc ggccgattcg
     7501 attgtgtcgc atgtcaccca gcagggattc gatttgatga tcatggggac gcatgggcgt
     7561 cggggcattt cccatgtcct gctcggcagt attgccggcg cgatgttgcg tcatgcacct
     7621 tgcccagtcc tgaccgtgct ccaggtcaac tttggcgcgg actacgagcg ggtcgtcccc
     7681 ctgggaaatt catgaatcga acaggttcac cggaaggagg cctccatgaa gcgtaaggaa
     7741 caatctcgtg cagctgctcc tccctctccc cgagccaagg aacaaaccgc cgtcaatccc
     7801 agtgcgttac ccgacggcat gtgggagcgc atttcccaga aggcgtatga cttgtggcgg
     7861 gagcgaggat cccgtgaagg gtttgcgttg gaggattggc tggatgccga ggccatcgtc
     7921 atggaggaga tgcatgaatc tcgcgaatga acgggtcgtc gggcggtcgg cgcctgtcaa
     7981 taaagacctg gggcggctga tttcgaggct cgatcagctc tccctgtccc cgggattcgc
     8041 gcagcaacgc cgtatcgctt ttgaacgggc gttgcaggtc tatgtcgaac tggggagcca
     8101 gtcgcccttg acgccgttgc gggaggaggt cgagctggcg gatctcctgc tctatgccga
     8161 cttttatccg gaagacggac agctcactct catcgaacaa ctgcgcgacg tcatcacgga
     8221 acacatcccc gaagaagagc gggtctggct ggatccgctg aaacattcgt acctggatct
     8281 tgtggaaatc ctcccgtcac aggcgggagc ggcgcgcaga agtctgaaat ctctgggaga
     8341 cggtcggctg tatgacatac cggcggagag tgtgccccgg gatgccgcac tgggatcggt
     8401 gttgttcaca cgtctggtgc gcgaaccggg cgatccggaa agccggttgg cggtgctggc
     8461 cggcccgatg ctggtcctat ccattcagga cgcgcgggcg ctgtacgaaa cgaccggtga
     8521 agaacggcgc gagatggaag tggttggagg ctcattcgaa ctcggagact ggccggagtt
     8581 tgccaagcgg ttcggccatc tcctgctgaa gaattttgcg cgcatgcggt tggccgcgtt
     8641 attggaagcc gtgagcgaca ttcggtatcg gacggaaact gacgcaccgt atttttatgt
     8701 catggccttg tacgagcatc atgaattcac attcatttcc gggggcatga ccgagctgga
     8761 gggctgggcg gaagacgcac cgcccgagca ggccgtgacc ggtagccgcc ggaaacttcg
     8821 ccgtttcgtc cggcgagggc agaaggaact cgtggcgcgc atcacggtga cggcgacgga
     8881 gctgttcctg gaatgtgact cacggggatc cttggacgag atcaagcata cgttagcggc
     8941 caccttcggg tattcgctgc attttcgagg cgaaattgcc caaccgcctg tacggcaggt
     9001 cacccaggaa gagctgtccg gtaacgaacc gctgaccgtg gtggtgaccg gcgaagaaga
     9061 tcgatcgctg gtgaagggtt tccttgagac ggtgtatctg gaatgggcgg accgggaatc
     9121 tcctgcgttg ggcggcgata ctccgcggca tacggccaaa acgccggagg ggcgcgaccg
     9181 ggtggccgcg ctcatcgacg acatggaacg ctgtgatttc ggtctcgtcc gcagcggtat
     9241 cgcagcgttc gactacaacg tcttacgagc gcatgtcggc ttgtgctgat gtgagcacgg
     9301 agttcctgtg gcgcaagaca gacggacgat gatggacgga ggggagggca tgcaggagca
     9361 ggtcgaaagg attgtgctgc aggctcgcgc cgagccgccc aacgtgctga agacgcttct
     9421 ggccattgaa gagcacctgg ggcatgtgcc ggccgaagcg attccggtca tcgcgcggcg
     9481 acttggcgcc acgaccgcgc aaatcgccgg ggtgctgtcc tactatcccg atttgcgggt
     9541 gacggccccg ggccgccatg tcattcgcgt ctgtatggga gaatcgtgct atgcaaacgg
     9601 ttgtggccgg gtcttgcggg agctgcagga tcgtcttcgc accgacgtag gcgaaacgac
     9661 ctcaggagga aagtttaccc tcgacaccat gtcatgcgcc ggcaactgtg ccgtctctcc
     9721 gacggtcgtg atcgatcgag acctgtacgg tcgggtgttg ccatcgcaag tcgagacgat
     9781 gctggaacgc tatcggtaac atgaccagcg caacgcgact ctacctttcc aatgacacgt
     9841 cggccagagc tgcgggggct ggtgtgttgg ccgacgcctg ggctgaacgc cccgaggtgc
     9901 agctcatccg catctcttca cggggagcat tttttctcga gccgatggtc gagcgcgaca
     9961 gtccggaggg tcggatcgcc tggttcaatg ttcaaccgga cgatctggca tccatactgt
    10021 catgcaacgg cgggacaccg gtccgggcca ttccctttct cgccaatcaa acccgcttca
    10081 cttttgccaa cttcggcgag acagaaccct tggctctgga tgaataccag gcgcgcggcg
    10141 gcctgaaggg actggaggag tccttccgtc ttacgccgga tgcgatcatc gaggaactcc
    10201 gccagtcgca gttacgagga cgtgggggag cggcctttcc cgtctggaat aaatggaagg
    10261 tggcgcaaca gacgagagcg tcagaaaaat atgtggtcgc caacgccgat gaaggggatg
    10321 ccggaacgta ctgcgatcgc atgatcttgg aaggggatcc gtttcgattg ttggaaggca
    10381 tgttgatctg tgcccgggcg atcggcgcct cccgcggcta tgtctattgc cggcaggaat
    10441 atcccgcagc cgccgccacc ttgcgcgcag cgattcacaa agccgatgaa gccgagctgc
    10501 tggaactacg aggcgaaccg tttcccattg aggtggtgga gggagcgggg tcctatgtct
    10561 gcggagagga gacggcgttg ctggaatcgc tggagggcag gcggggtgtc gtgcgggcca
    10621 ggcctcccta tccggcacag gcggggttgt atggccatcc gaccattgtg agtaatgtcc
    10681 tcacctttgc caccatccca cagattctgt cgcgtggcgg cgcttggcat gcgtctctcg
    10741 gcaccgaaca gtcgcgcggc actatcgcct tgcagttggg cgggcgggtg aaacatccgg
    10801 gactggtgga ggtgccgttc gggctaagcc tgcgtcaggt gttggaccag ttcggcggcg
    10861 ggatggcccc cggctcacgg ttcaaagcgg cgcaggtcgg cgggccgctg ggcagtctgt
    10921 ttcccgctgc cggattggat ctcccgattt gttatgacgc ctttgccaag gcaggcgcgg
    10981 tgctcggtca tggcgggatc gtcgtctacg accatgaaac tgacatggtt gatttggcgc
    11041 gccacttcat ggcgtttacc gcccaagagt cctgcggcaa gtgcacgccc tgtcggatcg
    11101 gttccgtgcg cgggcgcgaa atcctcgaac ggattcaagc cggcggcggc accgtcgagg
    11161 accttgccct cctggacgat ctcggcgaaa cgatgaagct gaccagtctc tgcgccctcg
    11221 gcggccgggc tccctatccg gtgctgacgg ccattgaaca ttttcccgtg gaatttagaa
    11281 ataagctgtc agcggtcaga cgtcagtcgt gactgaaagc tgaggctgag cgcgcatcat
    11341 tatgaaactt tccatcaacg gacgagtgat cgtggcgtcg ccgggtgatt ccatttatcg
    11401 agcggcgaaa caggcgggaa tcggcatccc cgggctctgt acctcagacc atttgaatcc
    11461 gttcggctcc tgtcgcctct gtctgtgtga agtcgagggc cagagcggga cgccggcttc
    11521 ctgcaccacg ccggtgcgcg aaggcatggt cgtgcacacg gagagcgaac ggctcactcg
    11581 tctgagaaaa catctcctcg agctctatct ctccgagcaa ccggcaggag atcgcgtgcc
    11641 ggaacccttg caggcgttgg ccagatcgtt ggggttgacg catgttcgtt atcttcagcc
    11701 tgccggcaga gtgcctgtcg tcgatcgatc caatcccttt tttgtcttcg acaatgcggt
    11761 gtgtatttca tgcgcccgct gtgtgcgagc ctgcgatgag atccaaggca cgcatgccct
    11821 gacgatgttg tatcggggat tctccagccg gccggcggca ggggctgcgg cgttgagcgg
    11881 agaggcggca gggtttgcct catcgaattg cgtctcctgc gggtcttgcg tgaaggaatg
    11941 tccgacgggg gccttgatcg agaagaccgt gcttgaagag ggcgagccgg tgcaagccgt
    12001 tcgcaccacc tgtgcctatt gcggtgtcgg ttgcacattc gatgctggtg tgcgggaggg
    12061 gcgggtcgtc cggatgggac cggccgatga cgggccttcc aatcacggcc atgcatgcat
    12121 gaagggacga ttcgggtgga cctacaatta tgcgccggac cggctgcgca cgccgcttct
    12181 gcggcagggc gacaagtggg ttgaactctc ctggcctgcg gccctcgatc gaattgctga
    12241 ggagtggagt cgcatcaagg aagcccacgg gcctgatgcg ctggcgacga tctcctccag
    12301 ccgcggcacg aacgaagaga attatctctt cggcaaattc atgcgctgcg tgatcggcag
    12361 caatcatatc gacaactgcg cacgggtgtg ccacagcgcg accgtgaccg gcatgatgga
    12421 aactctgggc gcgtcggcgg caaccaattc cattcacgat cttgatctgg ccaagctgat
    12481 cctcgtcgtc ggagctaatc ccaccgaatc ccatccggta ctcggcgcgc gcatcaaaca
    12541 agcagcccgg cgcggggtgc cgttgatcgt gattgatccg cgacgcacgg aattagcccg
    12601 gctggccgat cttcatcttc aactgcatcc tggtaccaac gtggcgctgc tgaatggcat
    12661 gggccatgtc atcgcgaagg aacatttgac cgatgcggca ttcgtgcgtg accggaccga
    12721 ggggttcgag gaatggctga atgtggtgga gggttgtact cctgaactga cgtcgaggct
    12781 gaccggcgtg cccgctcatc tcatcaccga agccgcgaga cgatatgcca caagcggcgg
    12841 ctccatggcg gtgcatggcc tgggcatgac cgaacatcgc tggggaagcc atggggtgat
    12901 ggcgctggtg aatctcgcgc tggcgaccgg caacatcggg aagcccggta ccgggatcaa
    12961 ccccttgcgc gggcagaaca atgtgcaggg ggcatcggat gtcggctgcc tgcccacatt
    13021 ctttgccggg tatcagccgc ttacggatcc agcgctggcg gcggcgcatc tcgcagtcac
    13081 aggacggccc ttgccaacgg cccgcggcat gaagacgccg gacatgtggg atgccgcgct
    13141 ggcgggaacg ctcaagagtc tctggattat cggatatgac gtggctcaga ccgacccgaa
    13201 tctgaaaaaa gttcatgcgg cgctcaagca actcgatttt ctcatcgtcc aggatttgtt
    13261 tttcagcgaa acggccaagc tggcgcatct cgtgattccg ggggcctcat ttctggaaaa
    13321 ggatggaacc ttcacgaatc tcgagcgccg catccaacgc atcagaaaag ctgtggagcc
    13381 gccagacggc gtgttgccgg actggcaggt cgtctgcgaa gtctccgcgc ggatggggta
    13441 tccgatgcag tattcccatc cctcggccat catggatgag atagcgcaac tcgcgccgct
    13501 gtttgccggg gtgtcgttcg atcgactgga tgcgccggag ggattacagt ggcccgtgcc
    13561 ctcgaaggac catcccggca catcattgat gcatgaacag tcgtttccca aaggcaaggc
    13621 gaagtttgtg gcagtggatt acctgccgcc gggggagaca ccgagcgaag cctatccgct
    13681 ggtattgatc accgggagga ttctgcagca ttacaattgc ggggcgcaga cgaggcgaac
    13741 ggagatcatg caggtggtcg ataccgatgt gctggagatg catgcgctcg atgcgaccag
    13801 gttggacgtc cgtgacggcg atccggtcag gctgatcagc gcgcgcgggg aggccaggct
    13861 gcctgtgatg atcagtgacc gcgtgcagcc gggagagttg ttcacgagtt tccatttccc
    13921 cgattcggac ctgaacgtgc tcctgtcatc cagcgccgac gaaagttcga aatgtcctga
    13981 gtacaaagtc tcgacggtgc gggttgaaaa agtgctgtcg accgggacgc ccactccctc
    14041 catgcatgtg atgttgatta catgactgcc gagccagttc cttccgccac cggctcggtc
    14101 gccgacgcct atccgccccc tgaaatcgcc gcgcgtgtgt gcaagctcgg gctggccaag
    14161 gtgaacacgg cggtgccgac gatggtagcc ctggccgtgc tggccggcgc gttcatttcg
    14221 ttgggcgcct tgttttacac cgtcacgatc acggcgggac aagacggttc gccgttgcca
    14281 ttcggactct tgcggctgac cgggggcgtc acgttcagcc tgggcctgat tttggtcgtg
    14341 gttggcgggg cggaactgtt taccggaaac aatctgattg cgatggcctg ggctgccggt
    14401 tgtgtgcaga cgcgtcaggt cgtgaggaat tggatctggg tctatctcgg aaacttgctg
    14461 ggagcctgcg gaacggcggg actggtgttg cttgcgggga ttcagttgct aggggacggg
    14521 gctgtcggcg aggcgatggt gcgtatcgcg cgcagtaaaa tcgccctcga tccgctgtct
    14581 gcctttgcgc gtggcatttt gtgcaatgta ctggtgtgtc tcgcggtctg gttgtgtatg
    14641 ggtgcgcgga gcgtggcgga taagattctc gcgattgtgt ttcccatcag cgccttcgtc
    14701 gcttgcggct tcgaacattc cgtggcgaac atgttctttt tgccgctggg gatagcgctg
    14761 gcggcgggca gtgcggcgcc cctgtcgctc atagacgccc tgagcaacct ggcgctggtc
    14821 accctcggga acatcatcgg cggaacagtg ttagtcgcgc tagtctattg gttcatctac
    14881 ctcaggaccg gttcccagcc acgatgaaga gcatcctgtg gtttatgttg gcaggggacg
    14941 gcaggtagag ttcgggcggc taggataccg gtcagcgggc ctccaaccct tctttaatgg
    15001 ctttcgccac gtcaccgcat tcggtcacga cctccacgcg gtattgcggc cctgggctgc
    15061 gaggtgtccg gttggcgacg caggattcga agtctttgat cgcgctgttt tcgaaggtgc
    15121 gactccagat ttcggagacg cgcgcgcgct catcctgttg atgctgatcg agagccagcc
    15181 agccgaccag tgcgattccg gccaccaaca atgccatgcc gagcaccttg accggcttgc
    15241 tgcgcaggac tttgaggaga aaatacaggg ccgctccgcc gaccagaagg gccgccacaa
    15301 gggcgatcgc catctccgtc ggcgacagtt cgaggtatcg ggaattccac gtgaagccta
    15361 agtttgtcac gttcatctca tccctctcgt tttgctactt ataccagttc agctatacaa
    15421 aagcctcctc tgattcatac cggagaagtt tggcgggaag agatcgtaca gcgctcgagt
    15481 ttccggcggc ctgtaaacag tatcgttccg gatccttaca gaaagaatca cgattctgat
    15541 gccgtcagcg tctcgggcaa tggtatgctg cacctcgtga gcctgctgtt ctcttgatcc
    15601 tatgagccat tcatccattc gcagctatct gaccccacgc ctcggcatca tgctgccgct
    15661 ggggtttgct tcgggtctgc ccctggccct cacgggcgga acgttgcagg catggctgac
    15721 ggaggccggg ctggatctca cgacgatcgg cctgttcgcc tatgttggcc ttccctatac
    15781 cttgaaattt ctctgggcgc cggtgatgga ccggatcgtg ccgccctggc tcggccgccg
    15841 tcgcgggtgg atgctcgtca cccaaagcgg tctcgcgctt gccctgactg tgatggcctt
    15901 gatcggcccg gctgagggcg cgcaggtgtt tgcggccttg gctcttgccg tggcgtttct
    15961 ttccgcctcg caggatatcg tgttcgatgc gtaccggacc gacctgctga aacccgagga
    16021 acggggattg ggcgcggcga cctgggtgat gggctaccgg attgccatga ttgcatccgg
    16081 ttcgttggcc ctgatcctgg ctgcccgctt gaactggtcg gcggcgtatc tctgtatgtc
    16141 cgccttgatg gcattgggta tcgtgacgat cctcctgagt ccggaaccag aggatgcgca
    16201 ggtggcgccc aaatcgatgg cggaagcggt ctgggggccg ctgagcgagt tcctttcccg
    16261 tcctatggcg ttagcgctgc tcggtttgat cgtgctctat aaacttggcg atgcgttcgc
    16321 cggcgcgctg acgacgtcgt ttttgctgcg cgggatgcag ttttcttccg aggatatcgg
    16381 ggtggtccgc gcattcggga tgggagccac gattctaggc gcgtttatcg gcggcgggtt
    16441 gatgccgcgt ttagggctgt tccgttcgct gtttgtgttc ggtgtcctgc aggccctctc
    16501 gaacctgtca tttatgtggt tggcttgggc ggggaaaagc tatgccgtga tggcgtttgc
    16561 ggtgggagcc gaaaatctga ctggaggcat gggcactgcc gccttcgtgg cgctggtcat
    16621 gtcgctctgc aatcatcggt ataccgccac ccagttcgcg ctgctgtcat ccgttgaagc
    16681 gctcggtcgc gtgttcctcg gttggcctgc ggcgaaactg gtggggctgg ccgggtgggg
    16741 tccgtttttc tttgtgacct tcctcgccgc gctgccgggt ctgtggctgc tgtgggtgct
    16801 tcgtaagccg gttgcccaac atgcggaact gaatgcgggg aggtccgcgg ccggagcgcc
    16861 ctgttagttc ctgagcaaaa ctcttcccgc aggtgtgccg tctgttccta tttcatcttc
    16921 ctctgcagaa acaaaataaa cagcatcagg gtcggcagcg gtgccaatgc gaacggcacc
    16981 agccagaaga acacgttttt cccgcgcacc cgggcttggt cgaacatcca tacaaacagc
    17041 cacaccagca ggccgatata gttcaccgcc atccagcggg tggtgtggat cttcaagccg
    17101 ctgattgttt ccggctctcc ctgaagcagg aagattccga tcagcaggac gaaacctgcc
    17161 agcagaccgg ttacgaggcg tttactccaa acgaacatag tgcatcctcc gtgactgtcg
    17221 aaccggcgcg atcacgcgcc ttcgtattcc attccttgga aatcgggcgt aagctaaaca
    17281 gacgaggtgc atttgtcaaa ccgtggttgc tcccggcgcg caacgatcgt gcggcggcaa
    17341 ggtgaaggtg tgtgatgcgt actgggaccc tggttggatt ggtgggaagt ttgatgctgg
    17401 cggtgtggct ggcggcgacc aatccgacga tggacaccta tctgcagttc gtcgaagcgc
    17461 gcttgtccgc tgagatcgat aagatggatc aatccggccc gcgccgcgac cgcgatctga
    17521 ttcgttcgat tttccgcgca caagggccga agctcgtcga aggagtcgtc cggcccaata
    17581 cgagacggaa caactggggc ctgctcagtc tgtttgaaac gaatgtgctg gaccaaccgg
    17641 tgctggtgct gggcatcgga ggccaatttg tcccgttacg aggggtcgag gaggcgacgg
    17701 tgaaggtcgg tcggctcgca ttttgatttg tgccagcatt tttctcgctc ccctgtgaaa
    17761 aacttcctcc acagtctctg aacgcctgtg gataactcag caaaaaatgg agaaaagctg
    17821 catgaaacct agcggagacg ctatcaccag cttatcccca ttacccacag gtgtgccaca
    17881 acattttgtt tgacaaaaaa atgggatcac tatatctagt atgcaatgca gtcgagagag
    17941 tgtttcgacg cgaaatgtac ctctggatgg acaggactct ttcccatccc caaccacata
    18001 tacttgccga ccggcacctg caccgaacgt taggcgtctc ttaaggagga accagtgaga
    18061 attgaacgaa gattcaccaa gcgtgggcag agcccctatg agggtcttgc gttcgtgaaa
    18121 cgttcctcgg agatccggaa tccagacgga tcgaccgtgt tcaagttgga gcatatcgat
    18181 atcccggaac attggacgca attggcgatc gatattctgg cgcaaaaata tttccggaag
    18241 gccggagtgc cgcaagtgca cgaggacggc acgccggtgg tggacgccgc aggcaaaccg
    18301 gtcttgggag gggagcgtga ttcccgtcag gtgttcaacc ggttggcggg atgctggacc
    18361 ttctggggca agaatcacgg ctatttcaaa acgccggaag atgcgaccgc cttccacgat
    18421 gaaatgtgct acatgctggc ctatcagatg gctgccccca acagcccgca gtggttcaac
    18481 acggggttgc actatgccta cgggctttca gggccggcgc aaggacactt ctacgtcgat
    18541 cccaagctcg gcgaggtcgt gcgggcgacc aatgccttcg agcatccgca gccgcatgcc
    18601 tgcttcattc agtcgattga agacgacctg gtcaacgaga acggcatcat ggacctgtgg
    18661 gttcgtgaag cacggctctt caagtacggg tcgggcaccg gcacgaactt ttcgcggttg
    18721 cgtggcgatg gtgaatccct gtcgggcggc ggacgttcct ccggtctgat gtcgttcctg
    18781 aaaatcggcg accgcgctgc gggagcgatc aagtcgggcg gcaccacgag gcgcgcggcc
    18841 aagatggtct gcctggatct cgatcacccc gatatcgaag aattcatcga ctggaaagtg
    18901 gtcgaagagc agaaggtggc cgcgatggtc accggttcaa agatttgcgc gcagcggctc
    18961 aatgccgtgc tcaaggcctg tcaatcggtc gatgcgcagg gacaggctca tctcgaattg
    19021 gatatgaagc agaatcaggc gctgcgcgag gccgtgacgg cagctcgccg cgatatggtg
    19081 ccggaggcgt atatccatcg catgttcgac tatgcgaagc agggctatac ccacttcgtg
    19141 tttcacgaat atgacaccaa ctgggacggc aaggcctacc agaccgtgtc tgggcagaac
    19201 tccaacaaca gcgtgcgtat cccgaacggc ttcttcgatg cgttggagca cgacggcgat
    19261 tgggaattgc gccgccggat tgacggcaag atcagcaaga cgatcaaggc ccgggacctc
    19321 tgggataaga tcgcctgggc ggcctggatt tgcgccgatc ccggtacgca gtacgatacg
    19381 acgatcaacg aatggcatac ctgtccggaa gacgggcgga tcaacgcgtc gaatccctgt
    19441 tccgaataca tgtttctcga cgacacggcc tgcaacctgg cctcgttgaa cctcgccaag
    19501 ttttttaacg ccgaagggga gttcgacctg gattctttcc gccatgccgt ccggttgtgg
    19561 accgtggcgt tggagatcag cgtgctgatg gcttctttcc cgagccgcgc gatcgcgcag
    19621 aagagttacg actaccggac attgggattg gggtatgcca acctcggcac cattctcatg
    19681 aaacaaagca ttccgtatga ctcccagaag gcgacggcaa tttgcggcgc gatcacggcg
    19741 atcatgaccg gcgaatccta tgcgacgtcg gccgaaatgg ctgcggagat cggcccgttt
    19801 cccggctaca accggaatcg ggagtcgatg ctgcgcgtca tccgcaatca tcggcgggcg
    19861 gcctatagtg cgccgcctga ggagtatgag ggcctgacga ttttgccggc ctcgattcaa
    19921 ccggaacatt gtccgccggc catgctcctg gcggcgcggc gcgcctggga tcgggcgttg
    19981 gagttgggca cggcctacgg cttccgcaac gcgcaggtca cggtcattgc ccccaccggc
    20041 acgatcgggc tggtcatgga ttgcgacacg accggcatcg agccggactt cgccctggtc
    20101 aagttcaaga aactggccgg cggcggttac ttcaagatta tcaaccagag tctgccgccg
    20161 gcgctgcagg cgctcgggta tacggaggcc cagatccggg acatcgtggc ctattgcgcg
    20221 ggccatcaaa ccttgaaggg cgcgccgttc atcaaccatg aggtgctgcg tcaaaaaggt
    20281 ttcgacgagg cggcgctgga acggctggaa ggatcgctgg cgcaggcatt tgagatccag
    20341 ttcgtcttca acaaatacac gttaggcgaa gagttttgcc ggcagaaact gggcatcagc
    20401 gaagcacagt tggcggatcc ggcattcaac atgctcaaga ccctgggctt cacccaggaa
    20461 gatgtggcgg cggcgaacga ctattgctgc ggcacgatga ccgtggaagg cgcgccgcat
    20521 ctcaagctgg aacatttacc catcttcgat tgcgccaacc gttgcggccg aatcggtcag
    20581 cgctacatcg ccgtcgacgc gcatatccgc atgatggcgg cagcccagcc tttcatcagc
    20641 ggcgcgatca gcaagaccat caatatgccg gcggacgcca ccttggaaga cgtgaaggca
    20701 gcctacctct tcgcctggaa gagcatggtc aaggccgtag cgctgtatcg tgacgggtcg
    20761 aagttgagtc agccgttgaa tgcctcttcc gacagcggta agagcgcgga ggcggctccc
    20821 agcgtggcta ccgtcgcgga aaaggtcgcc gaacgggtct tggtgcggta tctccacaaa
    20881 cgccgttcgc tgccggcccg acgcggcggg tatacgcaga aggccatcat cggaggccac
    20941 aagttgtatc tgcgtaccgg tgagtatgaa gacggcacgc tgggcgaaat cttcctcgac
    21001 atgcacaagg aaggcgcggc tttccgcagc ttgatgaata acttcgccat cgcgatttcc
    21061 ctcggtctgc agcacggcgt gccgctggag gagttcgtcg aagccttcgt cttcacccgg
    21121 ttcgagccga acgggccggt gaaattgaac gatcgcatca agatggccac gtcgatcatc
    21181 gattacattt tccgggaact ggccatcacc tatctcgagc ggaaagatct ctcccaggtg
    21241 caggaagatg atcttcgcat ggactccatg aagaaggatg agcaggatcc cgaatgcgtg
    21301 gaagaagagg tcgacatgac ggcgctcaag aaggcgtcga tcctgactga gcatctgccc
    21361 gtgcgacgga acggcatgaa cggcggcaat ggtcataccc agagagcggg gaatggcagc
    21421 gttgcccaca aattggaatt aaagcgtgaa accctgaccg tgacctcggt gcggcaggaa
    21481 gccaaagaaa agggatacga aggcgacccc tgccagaatt gcaaacagtt cacgctggtg
    21541 cgcaacggca cctgcctcaa gtgcatgagc tgcggcgaaa cgagcgggtg ctcatagcgt
    21601 agatcgacca cgggttagca gaacagcaag gccgcgatta ccgttgaaaa acgaggtagt
    21661 cgcggccttt tcttgcagcc ggttcatttt tgtcctttcc gccgactcgc tgccggagct
    21721 agtcaaacag cattcagtac ctggcattcc ccccctttcg agttaccaac accaggtaat
    21781 gtgcccggga ctttcctcgg ctaactttaa gtcatcaacg acgtcggcgg cccacaccca
    21841 gaggaggacg gatgaaaaag cgatacagtt cacgagcgtc agtgcagagc acctgcgtgt
    21901 tgtcctgtaa cggcttagtc ggccaggcgg aaatggtcaa cctctccgtg ccgggctgcc
    21961 tgctcaccac gcgtatgaaa ctcaaggtcg gtcagtacgt ggacctgcgg ctcacgttcc
    22021 ataccagccc cgcacctatg cagatcaagt tggctgctgt gcgttgggtc agtgacgggc
    22081 aggtggggct ggagttcatc cggatgtcgg aggcggatca gacgcgattg cgctggctgg
    22141 caggccatgt cgagcagcga aaaccccaag ccaactggag tgagcctgtg gtctgtctga
    22201 gtgccgccta tacctaaacc tcatcgaagg gacgggagcg acgcaagaac gggcagcaga
    22261 acattgttgc ccgttcacct cctcattccg tcgatgaggt ccaagttgtg cgaatcaccg
    22321 cacgaggcgc atgtcttccg gcacatcatt catcgcgaaa tgaacctgca taagtatctg
    22381 cgtcacgcgt gcttaaacca attccatcgt tgtttccata tggctcttcg atgatgggat
    22441 gctcgttttt cactcgccct agtcgaaatt gaatgcgcgg tcttggtcgc ggtaagcggt
    22501 cttctacgta ataggtttgt gcaattgtca acagggggcg cttgccgtat cgtggctcct
    22561 acgtcgttgg acaaaaggct tcaaccgggg gacgcgatga tgcttcgttc tatccgcaaa
    22621 gaagtcctcg aattcgcgaa agtgactgaa cgtctcctgg gcgccgcatc cctcactcat
    22681 gaattaaccg aggaggaatg cacactgatc gcgctttacg ccaaagactt gagtgcagat
    22741 cagcaaccct ggagcaaatt cattcctggc actcagcatc aagatccagc ctatccatcc
    22801 aagggtatgt gggagagcag cgcatagcgg tcaatggcaa tgtgagtgag atccatgtcc
    22861 ggagaattca ttctgcagaa aggagagcgt atgcaacccc taccgccaca gcctcccgtc
    22921 ggatcaccca taacgcctcc tccctctccc gctcatctcc aatgctccta ctgtgcaacc
    22981 gcaatgctgt ttctcggcta tacaccaagc gagcaatgtg cggaatggat ttgtcccaag
    23041 tgtaggatga tgaagattcg cccgctggcg atgctggcga cgatcttgga cagttccaca
    23101 accgcgagat ctgtcttgcc tgatccgacg cgagtcggtc caaacgccca gtgactgaat
    23161 tgcggttgcc gactcactct acggtgaggg gcaaccgctt tctcgttgcc tgggcctatg
    23221 atcaaaaaga tcccaccgtt tcattgcggc gatgacggga acttcactct aatctttccc
    23281 caaatcatct tctcaggtcg cgccaactcc aggtaggtca tgtgaggcgc gagtacacac
    23341 caccgtaccc ggctgttcag gcgaagattc gctgtcgatc gcgtcaccct gtaccgttgg
    23401 gcacaatagc ttccgactgc atagtactag ggttcatccg agttggacga ttgtgccggt
    23461 ctgctaccgt gtgtgtggtc cgcacgttaa acatgagaga ggtaaggttg tgtttactcc
    23521 ccctcacatc tcccgctgga tgaagtgggt cggcggtatt gttgttgtcc tggtgacggc
    23581 gatgctcctc gtcgcagtta tcgcggatga gccggcgcgg aagtatgtcg aagacaaagc
    23641 caatgcctcg ctgagcgtct atacgcttcg catcggcaag ctcgacctgc atccgctgac
    23701 cctctcgctt gatcttgaaa acgtgaccct cgtccagaaa cgccatcccg atcctccgat
    23761 ggtgcacatt ccaaaatggc atgccggcat tcagttgtgg ggcctgttga ccgggaaagt
    23821 tgtgagcgac catcgcattg atggaccgtc cgccaccgtg acgcgccccc aagccaagac
    23881 agaactcaag gaaacccgtc cgagcggctg gcaggacatc gtccgcaacc ttcttcccct
    23941 tcgagtgagt gatgtgaagg tcaagcaggc ccaggtcaca tactacgatc atcccaaagc
    24001 tcagccggtg aaactcaccg acctgcagtt tgaagcaagg gagatcagca accgcggcgg
    24061 cgaaggtcag gaatatcctt ccaccatcaa aatggaggca cggctgccac aggggggccg
    24121 cattaccgct gagggccatg ccaatgctct tgccaaaccg ctaccggctt tcaatgtgga
    24181 cttcagtctt gacgatatcg cgctgcggga tttcgtcggg ctcgcgggtg gctacaacgt
    24241 gcaactcgac cgaggcaaga tcaccgcgaa cggccatgcc gaatatgcgc cctggaagca
    24301 agtggcggat gtgcgggaga tccgtctgga aggggtgaag gccgactatg tctaccggcg
    24361 acatccaaga gatgaggcgc gccgggagga agccgtggcg gtcgccaaga aagcgaaaga
    24421 caatcctctt ctcgtcatca atgtgaagca gggcaagatc ttggatagcg agttcggctt
    24481 cgtgaatcag tccgtgaccc ctcattaccg ggtgtttgtc ggcgaggtca atgcagacct
    24541 ggacaatttc acgaccaggc tgcgggagtt gcaagggggc gatgcggtca tcaaagtgac
    24601 gggacggttt atggggacgg ggcggagcgt cattgtgggc accttccggc ctgaaaaacc
    24661 ctcccctgat ttcgatgtcg gagtccaaat cgtgaaaacc gaattgaaat cgttcaacga
    24721 tgtgctccga gcctacggcg acttcgatgt ccatcgaggc aacctttccg tcttcagtga
    24781 actgtccatc cgccacaacc aggtctccgg gtacctcaaa ccgatcttta aagatgtcga
    24841 agcctatgac cccaaccagg atgccgataa agcgtggaca aaaaaagtgt tcgaatccgt
    24901 catcgacggc atcgtgaaac tgctgaataa cacaagcgag gatcaggttg cggccgacgc
    24961 cggtatatcg ggccccgtga aaaatccttc tgccgacacg tggcaggtga tggggacttt
    25021 gatccaaaac gccttcttca aagccattct ccctggcttg gaaaaagaac acggcaaagc
    25081 ttgacccgca tgcaatccat ggcgcctcca cagtcaccgt tatgatgaga atgatcgctg
    25141 ccaaggacgt cgtcaagtca gcccgcacgg cagggctgcg gtatgtcaga gagggcatgg
    25201 gaggattcag gcgggtcgct agacgcggaa cgtcggtgta tctgaacagt tcgggcgtac
    25261 ggctgcgtga tgaacgacaa cttcaccgca tccgctcgct tgtcattccg ccggcctgga
    25321 cagaagtgtg gatttgcgga gatcccaatg gccatctaca ggcgaccggg cgtgatgcca
    25381 ggggcaggaa acaatatcgc taccaccagc gttggcgtga agttcgggat gaaaataaat
    25441 atggccggat gctcatgttc gccaggctgt tgccgcgcat ccggcaacgc atcgagcggg
    25501 accttgcctt gccgggtttg ccacgagaaa agattttagc cacagtcgta cgcctgctcg
    25561 aaaccacgct gatcagagtc ggcaatgagg aatatgccaa aaccaataat tcgttcggac
    25621 tgaccacgat gcgtgatcgc cacgcccgcg tcgaaggggc aagagtccga ttcgagtttc
    25681 gaggcaagag cggtgtgcaa catctggtgg atctgacgga ccgccggttg gcccgcatcg
    25741 tgcgacaaag ccaggatctg ccgggatacg aactctttca atacgtcgat gcggagggcg
    25801 cccgacatgg catagatgcg ggagacgtca atgattatct ccggcacata gccggggagg
    25861 agtgtacggc gaaggacttt cgcacctggg ccgggaccgt gctggccgcg aaggcgttgc
    25921 aagaatgccg cgccatcgga tcggcagcgc aggcgaaacg caatatcgtg gctgccatcg
    25981 aggtggttgc gaagcggctg ggcaatacca agaccatctg ccggaaatgt tatgtgcatc
    26041 cggaaatcgt gcaggcctac ttggaaggat cgttggctcg atccttgacg acgagaatcc
    26101 ggcgaccttt cgcacgcatg gcgggatgtc tcacgccaga tgaggcggcg gtactagcgt
    26161 gtcttgagcg ccgcctcggg cgcaaggcca gtcggggcat aggattgcgc tcatcggcat
    26221 aagggagagg aggatgcgtg tcccggccgt gcatccggcc ccattgatgt gtaccaagat
    26281 ctttgcaacg gagccgcatt cgtcagagaa ggcgtaggcg cattggataa atcaaatgtc
    26341 agggtatatg gcggtgactt gtatacgatg tgggagattc cagatctgcc acagtgctca
    26401 tgcgcgcatg ggctcgtcat tagcctggtc ctgcgagggg ctccgctgtc ttacctgcgg
    26461 atcgctcgca gacattccag ggtggggcga gcaggacgtc tctgatcggg gcggcatacc
    26521 tccgacacct gctcacgggt ctctttcgcc tgttcagccg tggggctggt tattgtttgc
    26581 tctgggcaaa ggcatgtaat ggctgagtcc cggcccatcc atgttgggcc atccacctgg
    26641 cgatcaggtg gtcgacagac caggccccgc caccttccag aagaagtgcc acactcatcc
    26701 cgatgatcaa caggtgatat tcaaatccct cgccctgctg ttgcccaaac caattcatga
    26761 agaagccgtt tggccagtgc accatggcaa cagctcccag catgatgagc aggtagctgg
    26821 cagccacaaa gcgagtgagg aacccggcca gcagactgag gctgccgatc gactcgccga
    26881 tgatgacgag aaaggctatg agccagggaa ggcccatgga agtggtaaaa aatccaatcg
    26941 tgcccgtaaa gccctgtccc ccaaaccagc ccaggagttt ctgtgcccca tgggcaaaga
    27001 tcacaatcgc cagggtcaca cgcacgatga acattcccac agagccgtga gtggtgaaga
    27061 gccagagcat gggtgacctc cacattgggg attgcatgtc gccggtgtcg ggacattttc
    27121 atctacaccg ttgaaatcac tctggctttc cgcttcaagc ccgtcgcgct gctggaagta
    27181 gatcgacgtc tggctgtatc gacgcactgc cagccgctgg actccttcct agatgttaag
    27241 cggccttgag ttcgttgtgc cgccgcttgt tgggaacagc gaggaagacc aatccatcct
    27301 ctcgagttac tgaccgagaa gtcgatctcg tacgaagctc aggtggaacc ccttgaatgg
    27361 cgccttcacc aatcgccgga caacaaacgg catgatgaga aaggcactga tcaaagtggc
    27421 aaaggccagg ggcaacaggg caatccatag tgtcggaact gtccgcgtca attcaggaat
    27481 gagaaagagc aagccgatgt ttccggcgaa catgaggaga acgaagctac ccactccgcg
    27541 ccacagcagg gcccagccga gccgcaccga ttcctcgtac gtcggctgtt cgttcgatat
    27601 ggtgtggacg gtatccatca tttcggatca cgcaaacgca acaacgtggg aagaaaaata
    27661 gatcgccgtt gggccgttgt tatctatgca ttgaccggct cgagaagcca ctaggcgtat
    27721 ccctcgtacg tcagcccaat tagtacgctg gtatctgttg tgctatcccg gcgtgagccc
    27781 ttatgcaaca tttctacacg cctttcgcgc ccgtcagcgg atcgcgcgtc caggcgtcag
    27841 cattggccta cgagtacgcc cgttcggctc atcctacggt cttcacgccg atgtttttct
    27901 agctggagat cacttaagaa atagcccctc gttttgggtg actcctttcc ggtaaagtaa
    27961 tcacagaaca tatgccactg acgtgcagcc atcgtgaaca tgactcacaa cacagggttg
    28021 cgcctatgtt gagaatattg atcggggacg atcatgccgt ctttcgccgc gggaccaaag
    28081 atgtcctgca agagcacttt gcgccgctcg aggtgcatga ggcaaagaca ggctgggaga
    28141 tggtgacgct ggcatctcaa gggaaatggg atgcgttcat catggatgtc acgatgccgg
    28201 gcaagtccgg gatcgatctg ttggagcaca tccgcagctt gcatccctcg acgcctgttc
    28261 ttgtgtacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg atctgttgga
    28381 gcacatccgc agcttgcatc cctcgacgcc tgttcttgtg tacagcatgc actctgaaga
    28441 aagttatgcc gtgcggatga tccgaagcgg ggccagcggg tacctgaaca aatcgacggg
    28501 actcgatgaa ctcgtggcgg ctatgcggtc aatcctcgca gggcaggaat tcgtgaatcc
    28561 ggcggtggcg cgctgtttga ttgagcgcat gcgcaccaag gattccggtc aacttcacga
    28621 tgccctgtcc aatcgggaat ttcaaattct caggatggtc gcgcagggtg aatcgctcaa
    28681 gcagattgca ctcacactct gtgtcagcgc caataccgtc agtacctacc ggacccgtat
    28741 tctccagaag ctccggctga agaccaacgc cgagttgacg cgttatgcgc tggaacatgc
    28801 gcttatttga cgggaccgca gagatccccg acagtcacgc acctcaggtc atttgttaaa
    28861 cgagtactca tcgcgccttc gcgtgagaag tgtcccggtt cgatacgggt aactggcgcg
    28921 tcttgacggc ccggcattca gggcagaacc aggtgaccgt gtgccctttg ggattggtcc
    28981 accagaacaa cacccgtgac gcacagcgcg gacattgcgt cgcaggggca gtctccttct
    29041 cggtcagggc cgtttgaaca tgcgactgtt ccatagatcc tcccgctggt tcttcgtggt
    29101 atcttcgaac accaggagcg tgtgacctct gaatcggcaa cggtgaaacg tgcggattgc
    29161 gaaccgtgct tcctctttcg tcgacatggt cacgcgcgca actcccagag gcatgccgtc
    29221 cgcatcaatg acgatctccg ctgccgtgac cttgccacaa gacgaaaaca acgcgacgat
    29281 gtcggcccgc gtgaccgtcg acgacaggcc gtcgatcaag agactcaggt cacatggagt
    29341 atgcaaggga cggctccacc caggtgcatt gctggaactc gctgtgcggt gagcgtacgc
    29401 ggtcctggtg caaatcgccg ccagcaaact aggaatattc ctgtcacggt ttcgtgacgg
    29461 ccagccgctt acattttcac tagtagccta gtaactctcc cagagtattg aggatgacgc
    29521 cttcactggg gcgtcgttcg aattcaaaga gttagctgtc gagagctggc aggacccgtg
    29581 tggcggtatt actggtattc accagggaat tggtgcgagc ttgccgtgat cggaaggacg
    29641 cggccgtcta agagtagcca cgcccatgtc gtacaggaag gaataacaca tgaactcttg
    29701 cgaaggtgga gcaggtaagg tgatcgtcct tacataggat gatcgttgcc gctggtcagc
    29761 ggcaggatca catgtaccat agtgccgact ccgatttgac tggtgacgtt caacgtccca
    29821 ttcagcaatt gcgcacgctc cttgaggctg acgagcccca accctcgagg ggactgtttc
    29881 atcgttttga cgtggaagcc gcgcccatgg tccgtgacgt agagggacaa ggtgttctcg
    29941 ttcttgatga gccgcacctc gacgcgtgat gcctgggcat atttcacgac gttgtgcagc
    30001 atttcttgta aggtcctgaa gaggcacgtc gaaatgactt cgggcacccg tctcgggaca
    30061 tcgatcgcgt gaaagtctac cggtagctcg gtcacttcaa tgaagtcctt caggtaactg
    30121 cgcatcgcct cttcaacacc catcgtgtcc cacatggctg gatgcaagcc ataggcgatg
    30181 tgccgcacgg attccagcgt tcgcttgatc tgttcattca gcatccgcac cttgtgcgtg
    30241 agcggctcgt cgcaacagcg ttcaatggaa ttgacggaga acatcatcat ggccaactgc
    30301 tgacaacagt tgtcgtggag ctcccttgcg atggcgcgtc gctcctcgtc ctgaatgttg
    30361 agcaactgcg cattcaggtc ttgaagtttt tgccggctgg tccgcaattc gtcttcctgc
    30421 accttcagtt gctgctcagc ctccttgcgg cgggtgatgt cgatcaccgc cagtcgcatg
    30481 atatcttttt gcccctgcgc cgggctgcct tccaacaaca cattcatcag ggctcgtggt
    30541 tggtcatcgg aggcatcgcc gagatccact tcgcagctca gacgttttcg ttcgaaacag
    30601 acttgtttgc aaaattcgat gaaccgccgg cgttgcgcga ccggcatgaa cagatggaac
    30661 cgccggccga tcaagcggtt catcggctgg tgcagcatgg ctgctccggc aggattcacg
    30721 tcgcagatgg ctccgcgcgg attgagcgaa aaatatccga caggaatgga ctcatagagc
    30781 tccacatagc ggttgcggct ttcctccaat tctagatgag tttgccgaag ctcttcgcat
    30841 tgaaggcgaa gtgccacatg gtgcactcga agttcatgta ccaactgcgc agtctcggtt
    30901 gcggactgat caatgggcgg tgtatgcgca ctttcgcgca aataggcttc tgcttttctg
    30961 cgtaggcgtt cgatttgggc ccctgatgtc ggcatagtgt cctctttacc tgcggaggat
    31021 gaagtggtca gaatgctggg gccggtcgaa aaaattaaac gggaccagcc ctttgatcgc
    31081 aagaggtgac aatggccgct gcgataccgg gaaagagatc cggataatga actgtgaggt
    31141 aaaacaccag ctgtattcta cggggttcgt tcgggacgta caatcggaga gtcaccctca
    31201 gcattcaacg ggctatacgt tggccacttt aatcacgtcg aagcgatcga tcattggatt
    31261 ttgctcttca ctggatcatg cgtcagtgca ataccgccat gatagtcgat cggaatcagt
    31321 ccgttcaccg cgtcatctcc aacacggcgc aatacgatgc cggcaaccct tgttctcgag
    31381 cgatgggccg cgaactggaa gatgggatca gatgtcgtgc ctggtgctgc taggctcctt
    31441 tggaatgcga cgaaacagca tgcgatcctg ggacagatcc tgatcatgac ctgtggactc
    31501 gcggcgcaca tggaggaggc ccttatcacg cccaacgtcg aacatgaacc ctgttggcag
    31561 ccctggctct ggttggcccg cgaatacggc taccgcgcct gttggtcatt tccggtgcga
    31621 accttcggag ggccgattct gggcacgctt gcgtggtatt tcaaagatcc tcgtgaaccc
    31681 tccttgcgta tgatggagtt ggcagccacg ttgacccatg cggccgccat cattatttcc
    31741 cgccaccggg aagcggagga acgaacccgg gtggaggcgg ccctcaggga atcggatgcg
    31801 cggtatcgcg ctttcgtcgc cgtcacatcg gacatcgtgt acaagatgag tgccgattgg
    31861 agtgagatgc agagcttggt cgggaaagag ttcatcgcca gcaccgaacg gccgcgccga
    31921 gactggatgg acgcctatat ccccgatgcc gaccgcacgg tcgtctggga ggccatcggc
    31981 gcggccattc actccaaaca ggcctttgaa ctggagcacc aggtcattag actcgacggc
    32041 acgatcggct ggacctactc acgggccgtg cccctcttcg atcagaaggg cgagatcgtg
    32101 gagtggttcg gcacagccag cgatattacg gagcgcaagc aggccgaggc ccagctcaag
    32161 acccgcaata tcgaactgga gcggttcaac aaggtcatgg tgggacggga actccggatg
    32221 atggagttga accacaagag ccctgggcgg caggggttgc agagtgaatc cccactggac
    32281 cagttcacca tcgaagcgca gaggccggcg gctgaaccag ggcagggtga tccaggcggc
    32341 gaatctcccc gtcgagcgtg accggcattc tggcagcaaa cctacgtgat ctctctgaca
    32401 tgtggttttc cctgaacagc cagtcctgat tgaccagggg cgggggtgag agaccatgag
    32461 aatgacggtc gaccagcagg tggtggcgag agaatggctg gcgcgtggct tcagttgtga
    32521 tctttgggtt gatccgccag ggcaagtatg ggaggattac cgccacgcga ccgatgagtt
    32581 ggtgatgccg gttgcgggcc ggttggaact ggagttcgag gggcagtgct tcaggcctgc
    32641 tccgggcgag gaaatcttca ttccggcctg tgtgtcccac acagtccgaa acatcggtgg
    32701 gaccactgct aaatggatct acgggtacaa gcgggttcat acaggctgat gagctaactc
    32761 agcagaggga gaggagccga tgccgacaac gacgcaattt gtggtgagtg gacagagcaa
    32821 gcccggtgtg ctggcctcag tggcggcggt gttgggagct gccggggtca acatcaaggc
    32881 cttttccgcg ccggaagtga ccgggccggg aaagctgcga ttgatcgtgg ccgatgtcga
    32941 tgccgcgcga atcgcgctga gaaaagccaa ggtcaaattc cgcgaagaga ccgccctgat
    33001 tctcagcctg gagaataagc ccggcgcgtt gaaacaagtg gccgattcgc tcaccagagc
    33061 ccgcatcaat gtgaagtgcg gctattgcac gccgtcgcgg gaaggcaaac gcgccatcgt
    33121 ggtgctgacc gtgtcgaaca ccaccaaagc cctcggggtg ctgcgcaccc attcactcga
    33181 cgaatt
//
LOCUS       DAOQDS010000045        32818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_462_length_32818_cov_20.093490, whole genome shotgun sequence.
ACCESSION   DAOQDS010000045 DAOQDS010000000
VERSION     DAOQDS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 32818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32818
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_462_length_32818_cov_20.093490"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PK589_12165"
     CDS             <1..221
                     /locus_tag="PK589_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20406.1"
                     /translation="IVTKDYLERVCRQYQAEKGTFGARTPVAVFRRPGSIAIVWKQHF
                     IDSPGDYVAEMVLVQQGGRYLVDHVFVL"
     gene            348..818
                     /locus_tag="PK589_12170"
     CDS             348..818
                     /locus_tag="PK589_12170"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNJ20407.1"
                     /translation="MTPWKRQGYALKSGEGPSIEFRGTKMTVKLSGASSEESHSLIEM
                     VHPSGVGPALHIHPTGAEAFYVLEGHYTIHCGEQQYEAAAGDFVYVPKGLPHLYQSGV
                     NGGKVLVLSPAGLERYFSEVAELLRVGPVTWDVEQAIAAQYGQEFLDGLRHWGQ"
     gene            complement(855..1502)
                     /locus_tag="PK589_12175"
     CDS             complement(855..1502)
                     /locus_tag="PK589_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20408.1"
                     /translation="MNADHPSSPSSPDMLVPEPSMNSTPADERSFHIRLAVFASVVII
                     AVVGAFVGYPTWQEYVSQQEAKLELRAYVNVRALGPPALKEGVSPRVRETIQNAGRTP
                     AYNDAIQAFITVADYPLIKNFERVTCPPDDSQPDKRKWFMGKVPRTETVRETPFTAEE
                     ITAIQTAKSALYYHGTVCYRDIFHESHRTDFCMFWKWDAGRLSPSLSCDQGNKTD"
     gene            1674..3035
                     /locus_tag="PK589_12180"
     CDS             1674..3035
                     /locus_tag="PK589_12180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNJ20409.1"
                     /translation="MGISPSLRGMSVIVAGAGLAGLTAAYELQRRGAQVTVVEARDRV
                     GGRVWTRRDGFAEGQHAEGGGDMIDPDQESIRRLAKDLGLTLSPILRGGFAFARQAAR
                     GPVITQASKAGDLWAQLAQAAEPWVRAHRLNERRWDGPIARQLAEQSVADWLDSIGAN
                     QQLRARLNGMRGFFLADPEHLSLLALVDQLSAESSALDHFYRIKGGNDRLATALADTL
                     NEPIQLKTIVCAVAQRRGQRRVTVRRGGQQDHITADAVVLALPATIVRRLAFSPPLPS
                     PQRKAFQDLQYGPATKTLLQFDRRFWRHQGAPLAYGTDLPIGAMWDGNEEQRGRSGIL
                     TLLAGGSASRDTQRLLAHGGAEAVAEQLKWLGATKAVLCASHHVSWEDDRFVRGGYAV
                     FQPGYDPEQRAWLSRPHGRILFAGEHTSLRWQGYMNGAVESGLRAALEVECLLGRLKS
                     NAH"
     gene            complement(3015..3659)
                     /locus_tag="PK589_12185"
     CDS             complement(3015..3659)
                     /locus_tag="PK589_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20410.1"
                     /translation="MWQSRNQIQIPDNSNGTRFDLTDIQGNGPNVQRRVELTWNLSHQ
                     HSLRFVYAPLGFSGTGSFGSPVRFSGGTFAPGVPIDSDYKFDSYRLTYRYLVHESERW
                     RWRIGATAFIRDARVELRQQGRVASDSNVGFVPLLSTSLEYDIAPRWTALLDFDGLVS
                     TQGRAIDAALKVRYDLTDHWFMTAGYRVFEGGVDNDRYAFGWYNFALVSVGVRF"
     gene            complement(3814..4395)
                     /locus_tag="PK589_12190"
     CDS             complement(3814..4395)
                     /locus_tag="PK589_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ20411.1"
                     /translation="MTAIVTIGTKRLRLRPFQTTDAEAVHRLAGSQDVAAGTFLPHPM
                     DRQAAQDWVTERLKDQAAGTGVTFAITLLENGELIGSIGMELMLVHEQGRLTYWLGRD
                     YWNQGYGTEAVTALVEYGFNSLSLHRIYAPHFHTNPASGRVLQKVGMTYEGRLREHYL
                     RFGQRIDVELYGMIRNEFLGLKVGEKGVADDHG"
     gene            4511..4717
                     /locus_tag="PK589_12195"
     CDS             4511..4717
                     /locus_tag="PK589_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20412.1"
                     /translation="MDVAQVLIAIVGFGAAVTTYCLMRFPHMRREPFRQYRGCSVLVS
                     SLAALVVLCLALVVLIAQYAWHAR"
     gene            5114..8422
                     /locus_tag="PK589_12200"
     CDS             5114..8422
                     /locus_tag="PK589_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20413.1"
                     /translation="MPSRMDLVRATHSLHKPIRAKTRAFDHHVETQGPPAQKPPSIPA
                     GLDRTDWMCAALFSLAVAIACLLIARAMPSFLFQSMDFWFEADTLREISNMTRVHDDH
                     YRTSVHPLFSLLTFVPVYLIKHGLSVSPLRAVLLVGSLVGGLWGGTLYVLFRLLGCRK
                     PDAIVLTLLGLVSASALFWLPVPTSYSWGSLSIMLALVLLLYTERRPIGASVYVVASA
                     LTLSFTVTNWMAGLLVSLARWPWKQAVQLSINALCLVVILWGVQKVIFPSAAFFIGQR
                     EEASFMNHPQAGGITNVASSIVFHSLVAPTVRFLKDDAYAQANADAFRLSERLTFQFS
                     TPGSGGPLGLVAVILWSGLLLNGLWRLVTLDHHLRFRLVLGSMLAFEVLLHMLYGEET
                     FVYSLNFMPLLLAVAACGALAPGRRIVTGVAAVLVVCVALNNWQQFQAATHTATQFTP
                     QRELMTAMMEKDPERPWPRSVGHVPLAVPGSPEGGTAYHEPGGDFSPQLPSFGVSLWL
                     CDATGAPVVVSQTVPIHEITQEFAPSAHPTIPAITTKTPYYQATWTRLDASRWELRFK
                     NETNHVPVIVLRSVGPAGGPVTALNQEEGKVTVNHRWAVTATPAPDRIAMGDENTAGW
                     MTEQSAVTSWSGEAGWGYARLQFSLGTTKPGEEIRVVLSDLRPPVEIEGYYKQAPKRA
                     RLALPDPRLQASMNAQITHLMMSLVDDETRPGDPAVFYRAWHRQGAYITAALARAGEP
                     RVSRVLSQFLAMHDFAGGSGPEADAPGLTIWALTESADYIGDKVHDEWLWPHILRKAR
                     RIEEMLTAKTPLVEPYTIPAPHDFKHLRQTRTAVLAQPAREGLIVGRVGEEWPSLYVN
                     AVSYRGLVAAAEFAERLGKRQQAARWRTQAQGLLESWQRQSTHDSMQGSASPVSLRAL
                     ADPSSRRHQLKQLLTTYRPTADHTTSAGQLSQAHQALRQGKPEAVWTVLHRLWGEQAS
                     PGLYTWEAALSSKDEVADGWQYARGWHNELAVTPDYETAALLLLLQQDMLAYLDEQAA
                     EPTVVIGAGITAGWLSQPMAVSNLALPGGSITWQWDGHAMRVTVRGPERAVRLGTAFP
                     PGTSLIVTHKPAIH"
     gene            8582..9604
                     /locus_tag="PK589_12205"
     CDS             8582..9604
                     /locus_tag="PK589_12205"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ20414.1"
                     /translation="MLKPEPAGGPTPQLFFQTANAYQRSQALKAAVELDLFTAIGLGH
                     DQVPALAQRCGAAERGVRILADYLTIQGFLTKEGDRYKLTPDSAVFLDRRSPAYMGSV
                     LGFLHAPKFIAAFEHLTEAVRKGGTVAGEAGTVAPEHPLWVDFARSMIPMMAKPAEEI
                     GIFVRQSQPNVKKVLDIAAGHGLFGVEIASRCPQAEIVALDWPNVLTVARELAQQAGI
                     ASRYRTIAGDAFQQEFGECYDLVLLTNFLHHFPVQTCESLLRKIHRSLAPGGRVITLE
                     FVPDENRVTPPPVAEFSLIMLATTPEGDAYTFREYEQMFAAAGFSRTELHPVAGSIEQ
                     VLVSTL"
     gene            9904..10896
                     /locus_tag="PK589_12210"
     CDS             9904..10896
                     /locus_tag="PK589_12210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HNJ20415.1"
                     /translation="MSDLIRLQPEGLVGPGNRFHIDAWGQAAVNLITHAHGDHARTGS
                     AEYWCAEPCAPILRHRLGPDLRIRAVPYRKPVRLDDCWISFHPAGHILGSAQIRVEQG
                     SRVWVVTGDYKRDADPTCELFEPVPCDTMITEASFGLPIYRWASTRSVTGRIVDWWQR
                     CREEGVTALLFCYALGKAQRILAELTHFTQEEVLLHGAVAALTQIYREQGVSMVPTRL
                     VSDAEGPTEGRLILAPPSAHRSPWMKRFRAVSTAFASGWMAVRGIRRRRGYHRGFVLS
                     DHADWKSLLDTVEESRATRVLVTHGTSDTLARYLRDVKGAKAEALTTHFGEGED"
     gene            10896..12623
                     /locus_tag="PK589_12215"
     CDS             10896..12623
                     /locus_tag="PK589_12215"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006701.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HNJ20416.1"
                     /translation="MQAFAILFEQLDQTTATNEKVRIMTEFFRHQDPDTSAWVLFFLS
                     GQRPKQIVGSTKLRRWAQEKVGYPDWLMEESYAAVGDTAELIALVLAAGEGAVKHAPL
                     SRLSLGEWMVQRILPLAGMDDQARRSNILAWWEELETNETFILNKLLTGSLRVGVSET
                     LVYRAVAAAFSLSPAVIASRLVGTWQPSAEFFLRLSAPAEETQTGQRSDETLLPLPFC
                     LAAPMEGDPEELGDISNWQCEWKWDGIRCQVVKSGSRLEIWSRGEERITGSFPDLSRL
                     LSPVSGDWVIDGEIVAGDWHRPDAFQSLQRRLNRKKPSLALIASSPVSFLAYDLLQAD
                     GRSWRHHPLAERQAMLERTLHPYIGERLGISPLLALSSWEQARLLRETSRVEGAEGLM
                     LKAKDSEYVTGRKRGVWFKWKIDPLAIDAVLTAAQPGTGRRASLYTDYTFAVWAGDSL
                     ITVAKAYSGLTDEEIRELDGWIRKHTRERFGPVRMVEARRVFEIGFEGISQSDRHKSG
                     FALRFPRILRERTDKRVEDADHVATLETLLASFGSSAGRPGRARARAESLPGQEQLQF
                     ELGAGDVGS"
     gene            12620..15181
                     /locus_tag="PK589_12220"
     CDS             12620..15181
                     /locus_tag="PK589_12220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HNJ20417.1"
                     /translation="MSSSAKVLRGIQRLNRMGPLRPRPDAEEQARARRKAWPTLSREE
                     AWKQWNEWFDRQGWSPFPFQVEAWQAFDAGRSGLVAVPTGSGKTYAAVGGPLLSLIAH
                     PIAGHQPPLTILYITPLKALARDIAQAIQRPLDDLGWPIDVDLRTGDTTATARRKFKE
                     RLPHVLVTTPESLAILLTDQGWKDRMRAVQAIILDEWHELLGTKRGSLLELTLARLRA
                     MAAGARVWALSATIANLEEAASIAVGSGLEPTIIRADIPRHIRIHCLLPESIQRYPWF
                     GYSGLSLLPEVVRQINPDQSTLLFTNTRSHAERWYQAILEARPEWSTVMALHHGSLDQ
                     DERQRVEDGIKSGALRLVVATSSLDLGVDFPPVENVIQIGSAKSIARAVQRAGRAFHR
                     PGEDTLVQLCPTNVMEILEASALRQGMQGGVLEERTPLEKPLDVLVQCLLNASFNEGF
                     DPADLCRQVRSAVSFRTVTDDEWSWALAFIRSGGALSSYPEFRKVEVVEGRYRFVSTE
                     AARRHKMNIGTILSDSGVTVQVRGGARLGMIDETFVAKLRPGDVIQFGGRTVQFLQMR
                     DMTAYVKPARQRSPVAAVWSGTILPLSVQLSFYLRREVDRLRQAVEGFNSRSPEIDAL
                     LPIARLQSSRSRIPSTAQTLIETCRTAEGHHGFVYTWEGWSVNEGLGHLVAYRLSRLR
                     PNTIAVSATNYGFELLSTEPLGSDREIGQALTTLDGLEEDIHASQNFPELARRAFREI
                     ARVAGLIYQGTPSARKTARHLQMSASLLFDVFRQYEPSHPLLAQAFREVHDRQLQIDR
                     LRGLMRRMGEQELLIVPIPRLTPFAFPLYVERIKSRLSTEKLEQRVARLQREVFR"
     gene            15182..15829
                     /gene="pdeM"
                     /locus_tag="PK589_12225"
     CDS             15182..15829
                     /gene="pdeM"
                     /locus_tag="PK589_12225"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HNJ20418.1"
                     /translation="MRIRIRDECVALLPERAMFWPTHSLLVVADCHLGKAETFQQQGL
                     WLPSQSGQQDLTRLSALVLSVDAQHVLFLGDLVHSLSGVTDAVVAEFARWLHEFSGSV
                     RVVLGNHDGGLARRWPKEWVKAERCDQVTIAPFRFKHQPIEGTVEADTFYWVGHVHPM
                     IQLAQGPDRVRLPAFVINDRQGVLPAFSSVSGGYDVPLRPDERRFAIGGDRIYEL"
     gene            16003..16449
                     /locus_tag="PK589_12230"
     CDS             16003..16449
                     /locus_tag="PK589_12230"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNJ20419.1"
                     /translation="MRYVIAIVVMCLSVATTVQYADARDRNGVFKASELIGMKVQGTD
                     GKNLGDIKDLVINPDDGAIDYAVLDFGGFLGIKDKYFAIPWDALQVASGKDKILIDAT
                     KRDLKNAPGFDKNHWPDFGDLAQTTVIYEFYGVPAPNTSGQHKATR"
     gene            complement(16481..18229)
                     /locus_tag="PK589_12235"
     CDS             complement(16481..18229)
                     /locus_tag="PK589_12235"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNJ20420.1"
                     /translation="MKPRRRPHFVHVLSSLIFLCLIESVSLLPADARSFVLSRFDVEL
                     QVLSGGELLVTETVSPRFEGAWNGIERLIPVEYRTPQGFNYTLFLDRISVTDEQGTPL
                     KFESSRERHYRNLRIWIPGAADATRTFVLKYLVRNGLKFFEDHDELYWNVTGDEWDVP
                     IEQASVRILLPPQATGVRAQAFTGAYGAREQAATVTILGSGIEMRMTRPLGFREGLTA
                     VVGWDKGLVAAPTVVEQTRMTLVSNWPLGIPLLAFLVMYRLWSTRGKDPRLRPIAVMY
                     EPPDQLTPAEAGTLVDESPDTRDITATVVDLAVRGYVRISERKAEHLFGLWSSMDYAF
                     QRTKPRQEWAALPSFERLLLEALFKDSEVDEILLSSLENRFYKSLPGIHDAIFQSLQK
                     RTYYSQRPDRVKQGYLIGGIAFGMLLTIGLGILADRWGLAPLTVFVAGLLSGMIVIGF
                     GRIMPARTQQGTRALEGVLGFEEFLTRVEADRFDRVIKTPELFEKFLPYAMALGVEKN
                     WAAAFETIVMTAPAWYQGSDLTQFNTRGFTSRMGDLASRTGSTMTSAPRSSGGSGFSS
                     GGSSGGGFGGGGGRGF"
     gene            complement(18255..18803)
                     /locus_tag="PK589_12240"
     CDS             complement(18255..18803)
                     /locus_tag="PK589_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNJ20421.1"
                     /translation="MGWIILAVLVALVLVVIGMYNSLVRLRAAAESAWADIDVQLKRR
                     YDLIPNLVETVKAYAAHEKGALEAVITARAKAMSAQGPEAKAVAENQLSQSLKSLFAL
                     AEAYPQLRAVEAFNQLQGSLNQVEDALQNARRYYNAVVRDYNAKRQEVPTNFIAGWFN
                     FAGRQFFELTETTQREPPRVQF"
     gene            19069..19395
                     /locus_tag="PK589_12245"
     CDS             19069..19395
                     /locus_tag="PK589_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20422.1"
                     /translation="MVHRNGRIGLGLVVLLWCVLAGGTLAVPLTRAEEPTIEFNSPDV
                     LRQILEQQVGKRVKVRLLSGQDVDGKVAKVGTQAVALTELSGMEFFDATIRLDQVAAV
                     IVRRGK"
     gene            19447..19956
                     /locus_tag="PK589_12250"
     CDS             19447..19956
                     /locus_tag="PK589_12250"
                     /inference="COORDINATES: protein motif:HMM:NF025196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HNJ20423.1"
                     /translation="MPVPILRRTVDLSGFPDLVVIYLGMRVNRWTGLKTLFGFGPQIA
                     ASVDAQPEGLLLHESLLWSLMPPHVGMRQYWRDFDALERWARSDPHRQWWQQFLRDTG
                     GTGFWHEAYFMRGGMEAVYNDILQDIGFLRFAPIRPAKGAQFTARSRAGRTGESALPP
                     AVLEEDLDR"
     gene            complement(19990..21375)
                     /locus_tag="PK589_12255"
     CDS             complement(19990..21375)
                     /locus_tag="PK589_12255"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNJ20424.1"
                     /translation="MGTAALALKDLWPCFEGIIPATIATCAKDGTPNISYVSQVHYVD
                     PEHVAISFQFFNKTRKNIAEHPFASVMVLDPRAMQAYRLSLTYERSESSGPLFQAMSL
                     QLQAIASHAGMSDVFRLRAADIYAVRHIERVDGFTSWPVPAHDERPPSLFIDPNLHAL
                     RVVAEQLNRAECLGELLETALRCLDEFFGLHHTMLLLVDEKRRTLQTIASRGYDENGV
                     GSEVGFGEGLIGMVAAAKLPLRLSGLDHTLRYARAVRDRAQALSGPESLCREIPLPGL
                     SNASTQIAVPLIIRHRCLGVLVAESLEPLTLLIREEAVMAIVAGHLAAGIEQSAQEAD
                     DSVAVEAPAAAAPPLHSPLSHRSFCFYQADDCIFVDGEYLIKNVPGRILWRILKQHQD
                     EHRVEFTNRELRMDSWLGLPEWKDNFETRLILLRKRLEQKCPDVRLVPRGRGRFALET
                     SVKIILSEKPA"
     gene            complement(21379..21834)
                     /locus_tag="PK589_12260"
     CDS             complement(21379..21834)
                     /locus_tag="PK589_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20425.1"
                     /translation="MIPPAIVDLLQTGVSVNVGTRDKNFMPECTRGWGIWVAANRTTV
                     TLLLTEPASARTLANLRDNGLMAITCSRPTDHVTCQLKGSVTQIRQATPQDYERQQQW
                     RHAFLGELIAVGVPAEQTDALIMQPAVAIEMQVTGVFAQTPGPGAGEQV"
     gene            21976..22803
                     /locus_tag="PK589_12265"
     CDS             21976..22803
                     /locus_tag="PK589_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20426.1"
                     /translation="MKSHILLSLCLAVLAATPLTGQAQAAQPGNVTITATDYGFKSPE
                     QVPSGLTAIEVINEGAEVHHAQLVRLAPGKTPADFAAAMKADPEHPPIWATFVGGPNA
                     VVPGDRATAVMDLQPGQHLALCLVPNKAGVAHVAMGMARPFMVTETIGTRIAEPKPDV
                     VITARDFQFVLSKPITAGSHTIQLLNEGSQTHEVVLVKLAPAATAKDFIAAFEPGAVG
                     PPPGRPLGGIVGIEQGSRGLFTAMFDPGPYALICFFPDKKTGAPHFAQGMTWEFDVR"
     gene            22858..25479
                     /locus_tag="PK589_12270"
     CDS             22858..25479
                     /locus_tag="PK589_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ20427.1"
                     /translation="MAPYTMPMHDMMPSTVEPMMADRGAALLQVAEVIAAHHDLPSLF
                     QDLAQCLPVVAPFDFVGLVLHDAAKHVMQVHVLETAEAKRVTRRLDGLEIPMKDSASG
                     WVWEHQQPILIPSLAAESRFQVGMDALRGIGVQSVCFFPLTTAMRRLGAIGFGCLKPF
                     AFDEGTVSFLNQVAKLVAVAVDNVLHHQDLARDRDRLRLLLEVTESIASHHDQSLLLA
                     DLAKRLPQVVPFDFMNVVRHDPSRDVMRLWLLVTSEQSTIEPGLETPVDESPGGLVWK
                     TQEPLTVDDVEREPRFPALMTLFRANGVRSFCVVPLTTAHRRLGAMGFGSLRPRVYLD
                     SEIAFMKQVAQQVAVALDNALNSESTLAYQSQLTRERDRQRLLLEVNNAVVSHLDLDQ
                     LFPAVSACLRRVIQHDGSSLLLCDEETGRWRIHVLDFDRNENFIEEGRIEETTQSPSC
                     LAITTGKAALFNERDLLAMGGASPCAQDLLDRGIKSFCSVPLLSRNRSIGALNVGRRR
                     DAGFDPEDVQLLGQVAQQIAIAVENALAFREIAALKDKLTKEKVYLEEEIQTDYNFEE
                     IVGESRALKRVLNQVQTVAATDSTVLILGETGSGKELIARALHNLSARRERTFVKLNC
                     AAIPTGLLESELFGHEKGAFTGAIATKIGRFELADRGTIFLDEVGEIPLELQVKLLRV
                     LQEQEFERLGSTRTMRVNVRVIAATNRDLSHMVDAQQFRSDLYYRLKVFPVTVPPLRE
                     RVEDIPVLVRHFVQKFASRMKKHIETVPTDAMRALQAYGWPGNVRELENFVERAVILS
                     SGSELFVPTAELKRPVSPSNGSATTLEDAERDHIIKALRDTHWVIGGSSGAAARLGMK
                     RTTLQSKMQKLGIVRPQ"
     gene            25717..26670
                     /locus_tag="PK589_12275"
     CDS             25717..26670
                     /locus_tag="PK589_12275"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNJ20428.1"
                     /translation="MESVTELLEQHGAWVLFGGVLAEQLGLPFPALPLLVAAGVLVGT
                     GHLSWSGALIAALSATLLADIVWFLAGRWRGRPVLTLLCRIALEPDACVRRTEDFFRK
                     HGVQSLLVAKFIPGLSTIAPPLAGIVGLGLPVFLLYDALGAAVWAGSGLGFGLLFTEE
                     VALALTYSEQVVPALLLAAVVLLPAYIGWKAWHVRRQLQAVPRMTVADLLDKLAAPDP
                     PLLIDVRTRLAVEAEPGLPNALQMSLEDLARRHGELPRSRDLVLYCACPADAASAQGV
                     MLLQRKGFTRVWPLAGGLHAWRAIVDGPEPVPMIQVERLAV"
     gene            26706..27233
                     /locus_tag="PK589_12280"
     CDS             26706..27233
                     /locus_tag="PK589_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20429.1"
                     /translation="MEFTMPTTEQAHWGLRAMKTVALADGVVDESERHMLEAVQRVFG
                     THHDVDGLQPLAPEELAAGLPDQQIRRQLVNGLIVMSLIDQDVKRAEAELVERFASAL
                     QVTLPEVTNLRHVVKKELFHLRLDLVRRSMIAPLFRQMPTGRQSEFGTRQHPALPRKK
                     WRELWQKNRWFTAMV"
     gene            27339..28466
                     /locus_tag="PK589_12285"
     CDS             27339..28466
                     /locus_tag="PK589_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ20430.1"
                     /translation="MNRRGWIGSSLLMLTVIGIGVGLAVWKYGSIQDQAAASTNQPEP
                     MEVVTLAVARAVDHHQTATSIGTVLALRSITLRNELAGTVSQVRFTPGQIVEAGTVLV
                     ALDVSVEEADLRAQQAQAVLARTVLDRRRALTQDLAAAQEEVDRAGADLDVVQAQIAR
                     TKALIARKTIRAPFRARVGLADVHPGQYLNEGTQLTTLQGIDEAVHVDFAVPQHVAAG
                     LRHGDLVDVYPAGDTSVLQAKIVALDARIDPATRNAMVRARIDRSPSMPTPGAAVRIK
                     VRVGAPRSAVAIPVNAVRKGPGGDQVFVINSDGQGKSRAQVRHVESGAMSGDDIIVYA
                     GLAAGEQVAASGAFKLRDGVLVVAAGGPVAKSDATPSPEQP"
     gene            28484..31567
                     /locus_tag="PK589_12290"
     CDS             28484..31567
                     /locus_tag="PK589_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ20431.1"
                     /translation="MRSFTDVFIKHPVLAVVINLVIVLVGWRALTTLPVQQYPKIESS
                     SVIITTVYYGASAETVRGFLTTPIERVVSAISGVDYLESTSRAGISTVTVHLKLNHSS
                     TAALAEVTARLQQVRSELPAEAEPPAVEVQRADRPYASFYLSFTSPERTVPALTDWLL
                     RSLQPQFATLTGVQRVTIEGGRQIAMRVWIDPDRLAAYNLSPGDVQAALRRNNYLAAV
                     GRTKGNLVQINLLANTDLRSTEEFEDLIVTDRGGAIVRLRDVARVENGAEEAEMVAKY
                     NRTEGVYLGIWPLVGSNEIEVAHRLREEMDRIRPTLPKDIDMQLVWDGTMFMRSALEE
                     ITKTLGETVLIVATVVFLFMGSVRTALVPLVAMPVSLVGAAIFMYAFGFSLNLLTILA
                     IVLSVGLVVDDAIVVVENVERHVREGKSRIQAALLGSRELVGPIIAMTITLAAVYAPI
                     GFQGGLTGSLFLEFAMTLAAAVVLSGVVAITLSPVMSSYFVHPEGKEGRLTKMVNRVF
                     DATRAGYGRLLDGALEMRWAIVVVALLVMVAAWPLYQFSRQELAPVEDQSHISLFLEA
                     APDSTVAATNRDSLKVIDALRAFPEARFTWSLTSSWGGFGGLVAKDWRERSRSTEMMY
                     GQVYGAVSQIPGLRVFPRLDPPLPTPGQYDVELILESQLPAEQLMETTAAVLGAGWQS
                     GKFLYVDTDLKLDLPEARVLIDRERLADLGFDLARVGQELGTLLGGAYVNRFNFYDRS
                     YKVIPQIGDKDRATLDPLLDLKIKTPGGQLVPVSTFTRIETSTAPRTLNRFQQRNAVR
                     IFGGVKPGVTKDEALRVLETAAAAAAGSRATLDYAGESRQIRHEGSALIVTLGFAVVL
                     IYLVLAAQFQSFRDPLIVLLGSVPLAISGALVFSFLNLTTINIYSQVGLITLVGLIAK
                     NGILIVEFANTLQARGLSKTAALREAAMTRLRPVLMTSAATVFGHLPLVLVSGPGAAA
                     RNSIGMVLVTGMTVGTLFTLFVVPVFYSLIATRHQPAEELEPAEEPGLQLAGAKG"
     gene            31579..31884
                     /locus_tag="PK589_12295"
     CDS             31579..31884
                     /locus_tag="PK589_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20432.1"
                     /translation="MLKITSEQTGDSARLTLEGSLSGPWVTELERTWQTVRLSGIFAP
                     VVELVGITFISEDGKALLARMWREGAALVAHGCCTRHIVGEITGAAPVAGPGQCDPA"
     gene            31897..>32818
                     /locus_tag="PK589_12300"
     CDS             31897..>32818
                     /locus_tag="PK589_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNJ20433.1"
                     /translation="MRMIACVITLLLVTGCAVGPDYSRPDLQMPTGFRMAVSDQETQS
                     FANLPWWDLLQDEELRRLIRVALAENKDLQRAVASVEEFQSRLLISKMDFAPKADVTA
                     NAPSFGRKANFLFPGFPNPFNYYLQGNLSWELDIWGRVRRSNEAARGDLLAREEARRA
                     VVLQLVSGVAEAYFELLQFDMQLGVAQRTLKSWEESVRIAEARLRQGMISRIDADQFE
                     AERANAAARAAELTRQMVQKENQLSILLGRPPGQVARGRSLTEQVLPPEVPAGLPSEL
                     LQRRPDLVQAEQELAAATARIGVAKADRFPK"
BASE COUNT         6013 a         9442 c        10616 g         6747 t
ORIGIN      
        1 caattgtgac gaaagactat ctcgagcgtg tgtgccggca gtatcaggcc gaaaaaggca
       61 cgttcggggc gcgaacgccg gtagccgtct tccgccgtcc aggctccatc gcgattgtgt
      121 ggaagcagca ctttatcgat tcacctggcg actatgtcgc tgaaatggtt ctggtccagc
      181 aaggaggccg ctatctcgtc gatcacgtgt ttgtgctgtg acctgccgat tgcttccgct
      241 ggaagtccac atatcgtggg tgctgaccat atccgccttc cctgccgctc cctcatgaat
      301 tgaaacgtat tcacatgatc ggtaatcgaa ggttgaaagg cgatgtcatg actccatgga
      361 aacgacaagg gtatgcgctc aagtcgggcg aagggccttc gattgagttt cgcggcacga
      421 agatgaccgt caaactgtcc ggtgcctcgt cggaggagtc ccattcgctg attgaaatgg
      481 ttcatccatc aggcgtgggc ccggcgttgc atatccatcc gaccggcgcc gaggccttct
      541 atgtgctgga aggccactac accattcatt gcggcgagca acagtacgag gcggcagcag
      601 gcgacttcgt gtacgtgccg aaaggcctgc cgcatctcta tcaatccggc gtgaacggag
      661 ggaaagtgtt ggtgttgtcg cccgcgggac ttgaacggta cttttcagaa gttgccgaat
      721 tgttgcgagt cggcccggtc acgtgggacg tggagcaggc gatcgccgca cagtatggcc
      781 aggaatttct cgacggactt cgtcactggg ggcagtgaaa gggtgcgcga cgttacggtt
      841 ttcggcgttc ccgcctagtc tgtcttgttt ccctgatcac aggacagcga aggactcagg
      901 cggcctgcat cccatttcca aaacatacag aagtcggtgc ggtgcgattc gtgaaagatg
      961 tcccggtagc acacggtccc atggtagtag agcgctgact tggccgtctg aatggccgtg
     1021 atctcttcgg ccgtgaatgg cgtctcgcgg accgtttccg ttcgcggaac cttgcccatg
     1081 aaccatttgc gtttgtccgg ctgcgaatcg tccggggggc aggtcacgcg ctcgaaattc
     1141 ttgatcagcg ggtagtcggc cacggtgatg aaggcctgga tggcatcatt gtaggccggc
     1201 gtccgtccgg cattctggat ggtctcccga actcgaggcg acacaccttc cttcaacgcc
     1261 ggtggcccca acgcgcggac gttgacgtag gcacgcaatt cgagtttcgc ttcctgctgc
     1321 gagacgtatt cctgccacgt cggatagccc acgaaggcgc caaccaccgc aatgatgacc
     1381 acagacgcaa agacggcgag cctgatgtga aagctgcgtt cgtcagcagg cgtcgaattc
     1441 atcgaaggct cggggacgag catgtcaggg gaggaagggc tactcgggtg atcggcgttc
     1501 atgctcaacc tcctcgtttc aagactgtag cgtagcgatt ctggaatgtg ggatcaagca
     1561 gcccgtgcgc atccttccag aaatgtactg gattcacgtg aggagtgact gattgtcgcg
     1621 ccacctcaca tcgccctatc tctgagctgc tgaatcgcgt atcgtcgaga gtcatgggaa
     1681 tcagtccatc attgcgtggc atgtcagtga tcgtcgcggg ggcggggcta gccggtctga
     1741 ccgccgcgta cgaactgcaa cggcgcggtg cgcaggtcac ggtggtggag gcgcgcgacc
     1801 gtgtgggggg ccgcgtgtgg acgaggcgtg acggttttgc cgaggggcaa catgccgagg
     1861 gcgggggcga catgatcgat cctgaccagg agtccatccg ccggttggca aaggatctgg
     1921 ggctaaccct atcgccgatc ctgcgcggcg gctttgcctt cgcccgtcag gcggctcgcg
     1981 ggcccgtcat cacgcaggct tccaaggcag gtgacctctg ggctcaattg gcacaagccg
     2041 ctgagccctg ggtgcgagcg catcggttga acgagcgccg atgggatggg ccaatcgcgc
     2101 ggcagctggc tgaacagtct gtggcggact ggctcgactc tatcggggct aaccagcagc
     2161 tacgtgcgcg gctgaatggc atgcgcggat tttttctggc cgacccagag catctgtcgt
     2221 tgctggccct cgtcgatcaa ctgtctgcgg aatcgtcggc gttggaccat ttctaccgca
     2281 ttaaaggcgg gaacgatcgt ctcgccaccg cactcgccga cacactcaac gaaccgattc
     2341 aactaaagac catcgtgtgt gcagttgcgc agcgtcgagg ccagaggcgg gttaccgtgc
     2401 gcaggggagg tcagcaggat cacatcaccg ctgatgcggt agtcctggcg ctacctgcca
     2461 ccatcgtgcg tcggctcgcc ttcagtccac ccttgccgtc cccacaacgc aaagcctttc
     2521 aagatctcca gtacggacct gcgacgaaga cgctgttgca gttcgatcgg cgattctggc
     2581 ggcaccaggg agcgcctctg gcgtacggca ccgacctgcc gatcggcgcg atgtgggacg
     2641 gaaacgaaga acagcggggg cgaagcggca tcctcacctt gctggccggc gggtcagcga
     2701 gtcgtgacac gcagcgactc ctggcccacg gcggtgccga ggcggtggcg gagcagctga
     2761 agtggttggg ggcgacaaaa gccgttctgt gtgcatcgca tcacgtgtcc tgggaagacg
     2821 atcgtttcgt gcgcggcggc tatgcggtgt ttcagcccgg atatgatccg gagcaacgtg
     2881 cctggctgtc ccgtccgcat ggacggattc tctttgccgg cgaacacacc agcctgcgtt
     2941 ggcagggcta catgaatgga gccgtcgaaa gcggtctgcg agcggcgctg gaggtggagt
     3001 gcttgctcgg acggctaaaa tcgaacgccc actgatacca gcgcgaagtt gtaccagccg
     3061 aaggcatacc gatcattgtc cactccgcct tcgaataccc ggtatcccgc agtcatgaac
     3121 caatggtcgg tgaggtcata gcggactttt agcgccgcgt cgatcgcacg tccttgtgtg
     3181 ctgacgagcc cgtcgaagtc aagcaaagcc gtccagcgcg gagcgatgtc atactcgagg
     3241 ctcgtgctga gtagcgggac gaatccgacg ttgctatcgg acgccaccct tccctgttga
     3301 cgcagttcca cgcgcgcatc tcgtatgaaa gccgtggcgc cgatgcgcca gcgccaacgt
     3361 tccgactcgt gtacgagata ccgataggtc aggcggtagg aatcgaactt gtaatcggaa
     3421 tcgatgggaa cacccggggc aaacgtgcca cccgaaaaac gtaccgggga accgaacgac
     3481 cctgtgcctg aaaacccgag aggcgcatac acgaatcgga gtgaatgctg atggctgaga
     3541 ttccacgtga gctcaacgcg ccgttgaacg ttagggccgt tgccttgaat gtcggtcaga
     3601 tcaaagcgag ttccattgct gttgtccgga atctggatct gatttcgcga ttgccacacg
     3661 gcacctgttt caaattccag cgagaagcga tggtcggggg accttacttc ttgggtgaat
     3721 ccaagattgg gtagacctac tgtggctatg aaggagagcc ggatcacagc ccttgccaga
     3781 gcggcgtgag cttttcgctg actgtgcgag aatctaccca tgatcatcgg ccactccttt
     3841 ctcgccgact ttcagtccta gaaattcgtt ccggatcatt ccgtacaact ccacgtcaat
     3901 gcgttggccg aaccgaagat agtgctcacg cagacggcct tcgtatgtca tgcccacctt
     3961 ctgtaacacg cgtcccgacg ccggattcgt atgaaaatgc ggcgcgtaga tgcgatgcag
     4021 gctcaggctg ttgaacccgt attcgactaa agccgtgaca gcttcagttc cgtagccttg
     4081 gttccaatag tcacggccca gccaataagt cagtcgtccc tgttcatgta cgagcatgag
     4141 ctccatgccg atagagccga tgagctcgcc gttctccagc agggtgatgg caaaggttac
     4201 acccgttcct gccgcctgat ccttcagacg ttccgtgacc cagtcttggg ccgcctgccg
     4261 atccattgga tgtggaagga aagtcccggc agccacgtct tgtgatccgg cgagacgatg
     4321 cacggcctct gcgtcggtcg tttgaaatgg ccggagccgc agccgcttgg ttccaattgt
     4381 gacgattgct gtcatgggtg gctctcatta taccgactgg acagccacca ctgtcggctt
     4441 tcgtcgggat tcttgacgct cctcctgttc gcgggtaggg ttacccctgg tgtttccgta
     4501 aggagtttca atggacgttg cgcaggtgtt gattgccatc gtcggtttcg gggccgcggt
     4561 gacgacctat tgtctcatgc ggtttccgca tatgcgtcgg gaacccttcc ggcagtatcg
     4621 aggctgttcg gtgctggtct cgagtcttgc cgcgctggtg gtattgtgcc ttgcgctggt
     4681 ggtactgatt gcccaatatg cctggcatgc gcggtaacga aacctgcgaa ctgagcttcc
     4741 attcatggcc ccgcttcatt ctcctcaatc acgctcatcc gtccaattgt ggaacgatgt
     4801 gcctgaaact gaagcaatct gcgcttcctc aatctacaga aagagtgttg gtcatctttt
     4861 ctgtggcatc aagggttgat ctgacccggt cggtctgcta ggaataaacg cgacacctgt
     4921 gcagtcgacc ggacatacgc cgatcccttc gacggcgaga tgagacagtc actccttcgc
     4981 tcgtactccc ctcactccgc gtctcatcga tcgttcccgc ccgatccacg ggcctttccg
     5041 ttgtgtttgc tgacatcgtt cgtccctcaa cagcgtgctg aaaccgcgag ggatgttttg
     5101 tttttgaacc aggatgccga gtcgaatgga tctggtacgg gccactcatt ctttgcacaa
     5161 gcctattcgc gccaaaactc gcgcgttcga ccatcacgtc gagacgcagg ggccgccggc
     5221 gcaaaagccg ccgtccattc ctgcggggct cgatcgaaca gactggatgt gtgccgccct
     5281 cttttccctg gcggtagcga tcgcctgcct gctgatcgca cgagcgatgc cgtcctttct
     5341 ctttcagtcg atggatttct ggttcgaggc agatacgctg cgcgaaatct cgaacatgac
     5401 ccgcgtgcac gacgaccact accggacgtc cgtgcatccc ttattttctc tgctgacctt
     5461 tgtcccggtc tatctgatca aacatggatt gtccgtcagc ccgctgcgtg ccgtgctcct
     5521 ggtcggcagt ctggttggcg ggctatgggg cggcacgctc tatgtcctgt tccgtttgct
     5581 cgggtgccgg aagccggacg cgatcgtctt gaccttgctg ggactcgtca gcgcatcggc
     5641 gctgttctgg ctgccggttc ccacctccta ttcctggggc tctctgtcca tcatgctggc
     5701 gctcgtgctg ttgttgtata cggagcggcg tccaatcgga gcgagcgtct acgtggtggc
     5761 cagcgcgctg acgttgagtt tcaccgtcac aaactggatg gcaggactac tcgtcagcct
     5821 ggcgcgatgg ccctggaagc aggcggtgca gctttccatc aatgcgctgt gcctggtggt
     5881 gattctgtgg ggcgtgcaga aggtcatctt tccgtcggcg gcgtttttta tcggccagcg
     5941 ggaggaagcc tccttcatga accatcccca agcaggaggc atcaccaatg tggcctcctc
     6001 gattgtattt cacagcctcg ttgcaccaac cgttcgtttt ctgaaggatg atgcctatgc
     6061 gcaggccaac gccgatgcct tccgcctctc ggagcgtctg acctttcaat tttccactcc
     6121 cggctcgggc ggcccgctcg ggctcgtggc cgtcatcctg tggtcggggt tactcctcaa
     6181 tgggttatgg aggttggtga cgctggatca tcacctgcgg ttccggctcg tgctggggag
     6241 catgttagca ttcgaggtcc tgctgcacat gctctacggg gaggaaacgt ttgtctacag
     6301 tttgaatttc atgccgctcc ttttggcggt cgcggcatgt ggtgctctcg ctcccggtcg
     6361 acgcatcgtt acgggagtgg ccgctgtgct cgtggtctgt gtcgcgctga ataattggca
     6421 acagtttcaa gccgccaccc atacggcgac gcagttcacg ccccagcgtg agctgatgac
     6481 agccatgatg gagaaagatc cggagcggcc ctggcccagg tcggtcggac atgtccccct
     6541 ggccgttccc ggttcgcccg agggaggaac ggcctaccat gagccaggag gagatttcag
     6601 cccgcaactg cccagtttcg gcgtctccct ctggttgtgc gatgcgaccg gtgcgccggt
     6661 ggttgtcagt cagacggtac cgatccacga gatcacacaa gaattcgcgc catccgctca
     6721 tccgacgatt cccgccatca ccaccaaaac gccgtattat caggcgacgt ggacgcggct
     6781 cgatgcgtcc cgctgggaac tccgattcaa gaatgagacg aaccatgtgc cggtgatcgt
     6841 gcttcggagc gtggggccgg ccggggggcc ggtgactgcg ttgaatcagg aggaagggaa
     6901 ggtgacggtg aaccatcggt gggccgtcac cgcgacgccc gctcctgacc gcattgcgat
     6961 gggagacgaa aatacagctg gttggatgac cgaacagtcg gccgtcacgt cctggagcgg
     7021 cgaggccggg tggggatacg cgcggctgca attctcgctg ggaacgacga agccgggcga
     7081 agagattcgg gtcgtgctgt cagacttgcg ccctcccgtt gaaatcgaag gctactacaa
     7141 acaggcgccg aagcgggccc ggctcgcgct gccggatccg cgcctgcagg cttcgatgaa
     7201 cgcccagatc acgcacttga tgatgagcct ggtcgatgat gaaacgcgcc ccggcgatcc
     7261 tgctgtgttc tatcgggcct ggcatcgcca aggcgcgtac attacggcgg cactcgcccg
     7321 cgcgggggag cctcgcgtca gccgggtgct ctcacagttt ctcgcgatgc atgattttgc
     7381 aggaggctcc ggtccggagg ccgatgcgcc tgggctgacg atctgggccc tcactgaatc
     7441 cgccgactat atcggcgaca aggtgcatga cgagtggttg tggccgcata ttctgaggaa
     7501 ggcccggcgc atcgaagaga tgctgacggc gaaaacgccg ctggtcgagc cgtatacgat
     7561 tccggctccg cacgatttca aacatctgcg ccaaaccaga acggcggtgt tggcgcaacc
     7621 ggcacgagaa gggctcatcg tcggccgggt gggtgaggag tggccgtcgc tctacgtgaa
     7681 tgccgtcagt tatcgcgggc tggttgccgc ggccgagttc gccgagcggc tcggtaagcg
     7741 ccagcaggcg gcgcgctggc gcacgcaggc gcaggggtta ctggagagct ggcagcgaca
     7801 atctacccac gacagcatgc agggcagcgc gtcaccggtg agtctgcggg ccttggctga
     7861 tccttcgtcc cgtcgccatc aactcaagca attgctgacg acgtatcgcc ccactgcgga
     7921 ccacaccacg agtgccggac aattgtcgca ggctcatcag gcattgcggc aagggaagcc
     7981 agaggcggtc tggacggttt tgcatcggct gtggggcgaa caggcgtccc cgggattgta
     8041 tacctgggag gcggccttgt cgtcgaagga cgaggtggcc gacggatggc aatatgcgcg
     8101 cggatggcat aacgagttgg cggtcacgcc cgactacgaa acggcggcgt tgctcctgct
     8161 cttgcaacaa gacatgctgg cgtatctcga tgagcaggct gcggagccca cagtcgtcat
     8221 cggcgccggt atcaccgcgg gctggttgtc gcagccgatg gcggtctcca acctggcgct
     8281 ccccggtggt tcgatcacct ggcaatggga cgggcatgcc atgcgcgtca cggtgcgcgg
     8341 cccggagcga gcggttcgtt tgggaactgc gtttcccccc ggtacctcgt tgatcgtcac
     8401 gcataaaccg gctattcact gaagcgtgag tatttcttcc acggacgcgg ttgtgcgcca
     8461 ccagcgctgg tgccgtcgtt ctgctatcgc ccaccctcgc atccatcctc ccgacggggt
     8521 acaatccggt caatgccgag cgctcacatg gctaccacca ccgaaccgag gagtgttgcc
     8581 gatgttgaaa ccggaaccgg ccggggggcc gaccccgcag ttattctttc agacggccaa
     8641 tgcctatcaa cggtcgcaag cgctgaaggc tgcggttgaa ctcgatctct tcaccgccat
     8701 tggactgggc catgatcagg tgccggcact ggctcagcgt tgcggtgctg ccgagcgcgg
     8761 cgtgcggatt ctcgcggatt acctcaccat acaaggattc ctcaccaaag agggcgatcg
     8821 gtataagttg acgccggaca gtgcggtctt tttggaccgt cgctcgcccg cctatatggg
     8881 ctctgtcttg gggttcctgc atgcgcccaa gttcatcgcc gcatttgagc atttgaccga
     8941 ggcggtgcgg aagggtggca cggtggccgg cgaggcggga acggtcgccc cggagcatcc
     9001 gctctgggtg gactttgcgc gttccatgat ccccatgatg gccaagccgg ccgaggagat
     9061 cggcatcttc gttcggcagt cgcagcccaa cgtgaaaaag gtgttggata tcgcggccgg
     9121 gcacggtctc ttcggcgtcg aaattgcgag ccgatgcccg caggcagaaa tcgtggcgtt
     9181 ggattggccc aacgtgctga ccgtggccag ggaactcgcc caacaggccg gtatcgcatc
     9241 gcgctatcgg acgatcgccg gcgacgcctt ccagcaggaa ttcggcgaat gctacgacct
     9301 cgtcctgctc accaactttc tccaccattt ccccgtgcag acatgcgaat cgctgctgcg
     9361 taagatccat cgctcgcttg cgccgggcgg gcgcgtcatt acgctggagt tcgtgccgga
     9421 tgagaatcgc gtcacgccgc cgcccgtggc ggagttcagc ctgatcatgc tggcgacgac
     9481 gccggaaggc gacgcctata cgttccggga gtatgagcag atgtttgcgg cggcgggatt
     9541 ttcccggacg gaactccacc cggtagccgg gtcgatcgag caggtcctgg tgtcgactct
     9601 gtaataggcc ttccatgagt cgcgactcac ctcacgaccg gcaccggttg atcatccacc
     9661 ggtgccggtt tgggtctgac ccctactgtc tctaaggtga tcggcagcac ctgatccggc
     9721 agagtgtttc aatcagataa gcgtaccccc cttcgacatc actgcagacg ctgccggtat
     9781 tcctccccgt acagatcaat tcgccaccgc tagggttttg ggaatccctc tggcatgccc
     9841 ggtcattgtc taatgaagcg gttggtatga tgatcgaatg agcagctgga cggggacttg
     9901 ccgatgagcg acctcattcg tttgcagccg gaagggctcg tgggaccggg caaccgtttc
     9961 catatcgacg cctgggggca ggctgccgtc aatttgatca cccatgccca tggcgaccat
    10021 gccaggacgg ggagtgccga gtattggtgc gcggagccct gcgcgccgat tctccggcat
    10081 cgtctcggcc cggacctccg tattcgagcc gtgccctacc gcaagccggt gcggctcgat
    10141 gattgctgga tttcatttca cccggccggt cacatcctcg gctccgccca gatacgggtg
    10201 gagcaggggt ctcgcgtctg ggtcgtcacg ggggattata agcgtgacgc cgatcccacc
    10261 tgcgaactct ttgaaccggt gccctgcgac acgatgatta cggaagcgtc gtttggattg
    10321 ccgatctatc gctgggcctc gacccgttct gtgacggggc ggatcgtcga ctggtggcag
    10381 cgatgccggg aggagggagt cacggccctg ctgttttgct atgcgttggg aaaggcgcaa
    10441 cgcatcctcg ccgagctgac gcacttcacg caggaggagg tcctgctgca tggggccgtg
    10501 gccgcactca cgcagattta ccgcgaacaa ggcgtctcga tggttccgac caggctggtg
    10561 agcgatgctg aaggtcctac cgaagggcgg ctgatcctgg cgccgccttc cgcccatcgc
    10621 agcccgtgga tgaagcggtt tcgtgccgtg tccacggcat ttgcgtccgg gtggatggcg
    10681 gttcgaggga tccgtcgccg gcgtggctat caccgcgggt tcgtcctgtc ggaccacgca
    10741 gactggaaaa gtcttctcga cacggtcgaa gaatcgcgcg cgactcgtgt gttggtgacg
    10801 cacggcacct ccgacacgct ggctcgatac ctccgagacg tcaagggagc gaaggccgaa
    10861 gccctgacga cccacttcgg cgagggagaa gactagtgca ggcgtttgcc attctgttcg
    10921 agcaactcga tcagacgacc gcgacgaacg aaaaggtgcg gatcatgacg gagttcttcc
    10981 gtcaccagga tcccgacacc agcgcctggg tcctgttctt cctctcgggt caaaggccga
    11041 aacagatcgt cgggtccacc aagctgcggc gttgggcgca ggagaaggtc ggttatcccg
    11101 attggttgat ggaggaatcc tatgcagctg tgggcgatac ggcggagctg atcgcccttg
    11161 tgctggctgc gggagaagga gccgtcaagc acgcgccgtt gtctcgactc tcgttggggg
    11221 agtggatggt ccagcgcatt ttgccgctcg cggggatgga cgatcaggct cgccgctcga
    11281 acattctcgc gtggtgggaa gagctcgaga ccaatgagac gttcatcttg aacaagttgc
    11341 tgaccggatc gttacgggtc ggggtgtccg aaacgctcgt gtatcgcgcc gtggccgcgg
    11401 cgttttccct gagtcctgcg gtgattgctt cccggcttgt gggaacctgg caacccagcg
    11461 ccgagttctt tctccgtctt agtgcgccgg cggaagagac tcagaccggg cagaggtccg
    11521 acgagaccct gttaccactt cccttttgtc ttgctgctcc gatggagggt gatccggagg
    11581 aactgggaga tatatcgaac tggcaatgtg agtggaagtg ggacggtatc cgctgtcagg
    11641 tcgtgaaatc cggctcgcgc ctggagatct ggtcacgcgg cgaggagcgg atcaccggct
    11701 cctttcccga cctgtcgcga ctcctctcgc cggtatccgg cgactgggtt atcgacggcg
    11761 agattgtggc aggggattgg catcgtcccg atgcgtttca atctctgcag cggcggttga
    11821 accgcaaaaa accctcgctg gcgctcatcg cctccagtcc tgtttcattc ttggcctatg
    11881 acctgcttca agccgatgga cggtcatggc ggcaccatcc ccttgcagaa cggcaggcga
    11941 tgcttgaacg cacactgcat ccgtacatcg gggagcgtct ggggatcagt ccgctgctgg
    12001 cgctgtcatc gtgggagcag gccagactgt tacgtgagac gtcgcgtgta gagggcgccg
    12061 agggactcat gctgaaggcg aaggacagcg agtatgtgac cggccgcaag cgcggcgtgt
    12121 ggttcaagtg gaaaatcgat ccgctggcga tcgatgccgt tctgaccgcc gcccagccgg
    12181 gaaccggccg ccgcgcgtcg ctgtataccg attacacgtt tgctgtctgg gcgggcgata
    12241 gtctgatcac agtcgcgaag gcctattcag gtctcacgga cgaagagatt cgcgaactcg
    12301 acggctggat tcgcaagcat acgcgcgagc ggttcggccc tgttcgcatg gtcgaggcac
    12361 gccgcgtgtt cgaaatcggt ttcgagggta tcagccagag cgaccggcac aaatccgggt
    12421 tcgcgctccg gtttccccgg attctgcgcg aacgaacgga caagcgcgtg gaggatgccg
    12481 accacgtcgc gacacttgaa acgctgctcg catccttcgg ttcgtcggct ggccggccgg
    12541 gacgcgccag agcaagggcg gagtccctgc cggggcagga gcagctgcag ttcgaactcg
    12601 gggctggaga tgtcggttca tgagtagctc ggcgaaagtc ctgcgaggca tccagcggct
    12661 caaccggatg gggccgctgc gtccacggcc cgatgctgag gaacaagccc gtgctcgtcg
    12721 aaaagcctgg ccgacgctct cacgcgaaga ggcgtggaaa caatggaacg agtggttcga
    12781 tcggcaagga tggtcgccgt ttccatttca agttgaggcc tggcaggcat ttgacgcggg
    12841 acgctcaggc ctggtcgcgg tgccgacggg cagcgggaag acctacgccg ctgttggagg
    12901 gccgctcctg agcctgatcg cccatccgat agctggccac cagccgccac tcacgattct
    12961 ctatatcacg cccctcaagg ccctggctcg ggacattgcg caggccattc aacggccgtt
    13021 ggacgatctg ggctggccga tcgatgtgga tctccgcacc ggcgacacga ccgcgacggc
    13081 gcgccggaaa ttcaaagaac gattgcccca tgtcctcgtg acgacccccg aatcacttgc
    13141 gatcttactt accgatcaag ggtggaagga tcggatgcga gctgttcagg cgatcatcct
    13201 cgatgagtgg cacgaactgc tgggaacgaa gcgaggcagt ttgctggagt tgacgctcgc
    13261 ccggttgcgt gcgatggccg ccggagcccg ggtgtgggcc ctgtcggcaa ctatcgcgaa
    13321 cttggaggaa gcggcgtcga tcgcggtcgg gagcggcctt gagccgacca tcatccgtgc
    13381 ggacattcca cgacacatcc ggattcactg cctcctgccg gagtccattc aacgttaccc
    13441 ctggtttggt tattcgggat tgagtctgtt gccggaagtc gtcaggcaga tcaatcccga
    13501 tcaaagcacg ctgctgttta ctaacaccag gtcgcatgcc gaacggtggt atcaggcgat
    13561 cctcgaagcc aggccggaat ggtcgaccgt catggccttg caccacggat cgctcgatca
    13621 ggacgagcgg cagcgcgtcg aagatggcat caaaagcggc gccttacgat tggtcgtcgc
    13681 gacctcttca ctcgacctcg gggtcgattt cccgccggtt gaaaatgtca tccagattgg
    13741 atctgccaaa tcgatcgccc gggcggttca gcgagccggt cgcgcgtttc atcggccggg
    13801 agaggacacg ctggtgcagc tctgtccaac taatgtcatg gagattcttg aggcgtcggc
    13861 cctgcggcaa ggcatgcagg gtggcgtctt ggaggaacgg acgccgcttg aaaagccgct
    13921 cgacgtgctg gtgcagtgtc tcctgaatgc ctcattcaac gagggcttcg atccagccga
    13981 tctctgccgg caagtccgat cggctgtctc ctttcgaacc gtcacggatg acgaatggtc
    14041 ttgggcgttg gcattcatcc gttccggagg agccctgtct tcctatccag aattccgcaa
    14101 ggtcgaagtg gtcgaggggc gctatcggtt tgtttcgacg gaggcggccc ggcggcacaa
    14161 gatgaacatc ggcacaatcc tctccgacag cggcgtgacg gtacaggtcc gaggaggagc
    14221 cagactcggc atgatcgatg agacgttcgt ggcgaagctt cgacccggcg atgtcattca
    14281 gttcggcggc cgcaccgtac agtttctgca gatgcgggac atgaccgcct atgtgaagcc
    14341 ggcccgtcaa cggagtccgg tcgctgcggt ctggagcggc accattcttc cgctcagcgt
    14401 gcaactgtct ttctacctcc ggcgagaggt tgatcgtctg aggcaggcgg tggaaggatt
    14461 caattcacgg tcgcccgaga ttgatgcgtt gctgccgatc gcgcgtctgc aatccagccg
    14521 gtcacgtatt ccatccacgg ctcagacgct gatcgaaacc tgccggactg ccgaagggca
    14581 tcatgggttc gtgtacacct gggaaggctg gagcgtcaac gaagggctgg ggcatctggt
    14641 ggcctatcgt ctgtcgcggc tgcgtccgaa cacgattgct gtctcggcga ccaactacgg
    14701 cttcgagttg ctctccacgg aaccgctcgg cagtgaccgt gagatcggtc aggcgctgac
    14761 cactctcgac gggttggagg aggacatcca cgcgtcgcag aactttccgg agctcgcgag
    14821 gcgggcgttc cgcgaaatcg cgcgggttgc ggggctgatc taccagggca ctccgtccgc
    14881 gaggaaaacg gcacgtcatc tgcaaatgag cgcctcgctg ctgttcgatg tgtttcgaca
    14941 gtatgaaccg tctcatccgc tgctggcgca agcgtttcgt gaagtccacg atcgccagtt
    15001 gcagatcgac cggctgcgcg gcctgatgcg tcggatgggc gagcaggagc tgttgatcgt
    15061 tcccatcccg cggctgacac cgtttgcctt tcctctctat gtggagcgga tcaagtcgcg
    15121 cctctcgacc gaaaagctcg aacagcgggt cgcgcggttg caacgggagg tgtttcgctg
    15181 aatgagaatc cgtatccgag acgaatgtgt cgccctgctt cccgaacggg cgatgttttg
    15241 gccgacccat tcgctgctgg tcgtggcgga ttgccatctc gggaaggcgg agacttttca
    15301 acagcaggga ttgtggttgc cctcgcagtc gggacaacag gaccttacac gcctctctgc
    15361 gctcgtgctc agtgttgacg cacagcacgt cctgttcttg ggcgacctgg tccattcgtt
    15421 gagcggggtg actgacgcgg tcgtggcaga gttcgcgcga tggttgcacg agttcagcgg
    15481 gtcggtccgg gtcgtgctgg gaaaccatga tggcggactc gctcgccgct ggccgaagga
    15541 atgggtgaag gcagagcggt gtgaccaggt gaccatcgct ccatttcgtt tcaagcacca
    15601 gccaattgag ggaaccgtcg aggcagacac cttttattgg gtgggacatg tccatccgat
    15661 gattcaattg gcgcaaggcc cggaccgggt gaggctcccc gcctttgtga tcaatgaccg
    15721 acaaggtgtg ctgccggcct tttcatccgt cagcggcggg tatgacgtac ctctgcgtcc
    15781 tgacgagcgg cggttcgcga tcggtggcga tcgaatctat gagttgtgac ctttcgatgg
    15841 agagggcatg ccggcctcaa aaccgttcgc gctcctttga ggcgagacca tgccggctta
    15901 cccgtgcgtg tcgaactagg gtttttgaga ggcacgttcc tcctggcgtc gttgttaaat
    15961 ggcgcagagg tcgccttgca taggcgacga aaggagggcc ctatgcggta cgtcattgcc
    16021 atcgtggtca tgtgcctgtc cgttgccaca accgtccagt atgccgacgc tcgggatcgc
    16081 aacggagtgt tcaaggccag tgaactgatc gggatgaagg tgcagggaac ggacggaaag
    16141 aatctgggcg acatcaagga tctggtgatc aatcccgatg acggagcaat tgattatgcg
    16201 gtcctggact tcggcgggtt cctgggcatc aaagacaagt atttcgccat tccctgggat
    16261 gcgctgcagg tggcatcggg taaagacaag attctcatcg acgccacaaa acgggatctc
    16321 aaaaacgcgc cggggttcga taagaaccat tggccggatt tcggcgatct ggcgcagacc
    16381 accgtcatct atgaatttta cggggttccg gcacccaaca cgagcgggca gcacaaggcg
    16441 accaggtgac atgcaggtgc agtgacagtg cggccgcgcg ttaaaacccg cggccgcctc
    16501 cgccgccgaa tccgccgccc gatgatcccc cactgctgaa tcctgatccg ccggagcttc
    16561 ggggagcgga tgtcatcgtc gagccggttc gtgacgccag gtcacccatg cggctcgtga
    16621 atcctctcgt attgaactgc gtgagatcgc tgccttggta ccaggccgga gcggtcatga
    16681 cgatcgtttc gaatgctgcc gcccaattct tttcgactcc gagcgccatg gcatagggca
    16741 gaaatttctc gaacagttcg ggcgtcttga tgacgcgatc aaaccggtcg gcttcgacgc
    16801 gcgtgagaaa ttcctcaaac cccagcaccc cttccaacgc ccgtgtgcct tgctgtgtgc
    16861 gagccggcat gatgcggccg aatccgatga caatcatccc tgaaagcaag ccggccacga
    16921 agactgtgag tggcgcgagg ccccaccggt cagcgaggat gccaagcccg atggtcagca
    16981 gcattccgaa ggcaatgccg ccgatgagat atccctgttt cacccggtcg gggcgctggc
    17041 tgtaatacgt gcgtttttgc agggactgga atatcgcgtc gtgtattcct gggagagatt
    17101 tgtagaagcg gttttcgagc gagctgagga gaatctcatc gacttcgcta tccttgaaca
    17161 gagcctccag caagagccgc tcgaaggacg gtaaggccgc ccattcctgc cgtggctttg
    17221 tccgctgaaa cgcataatcc atgctcgacc agagcccgaa cagatgttcg gccttgcgct
    17281 cgctgattcg cacatagccg cgcacggcca ggtccactac ggtggcggtg atatcgcggg
    17341 tgtccggact ttcatccacc agtgtcccgg cttctgccgg tgtcagctgg tccggtggtt
    17401 cgtacatgac ggcaatcggc cggagtcgcg gatcttttcc cctggtggac cagaggcgat
    17461 acatcacgag gaacgcgagt agagggatgc ccaagggcca attgctgacg agcgtcatgc
    17521 gggtctgctc caccacggtg ggggcggcca cgaggccctt gtcccacccg acgacggcgg
    17581 tgagtccttc ccgaaagccg agggggcgtg tcatccgcat ctcgatcccc gaaccgagga
    17641 tcgtcacggt cgctgcctgt tcgcgcgcgc cataggctcc ggtgaaggcc tgggcacgga
    17701 cgccggtcgc ctgcggagga aggaggatgc gtacggaagc ctgctcgatg gggacatccc
    17761 actcgtcgcc ggtgacgttc caatacagct cgtcatggtc ctcgaaaaat ttcaggccgt
    17821 tccggacaag gtatttcagc acgaaggtgc gggtggcatc tgcggcgccg ggaatccaga
    17881 tccggaggtt ccggtaatgc cgctcccggc tgctttcgaa tttgagcggg gtgccctgct
    17941 cgtccgtcac ggagatgcga tccagaaaca gagtgtagtt gaacccttgc ggagtacgat
    18001 attccacggg aatcagccgc tcgataccgt tccaggcgcc ttcgaagcgc gggctgacgg
    18061 tttccgtcac gagcaactcg ccgccggaca acacttgcag ctccacatcg aaacgactca
    18121 gcacaaagga acgcgcatca gccggcagga ggctcacgga ttcaatgagg cacaggaaga
    18181 tcaggctgga gagaacgtgg acgaaatgtg gacgtcgtcg gggtttcacg atcggatcgc
    18241 cggcacgatc cacgctagaa ctgcacccgt ggcggctcac gctgggtggt ttcggttaac
    18301 tcgaaaaact ggcggccggc aaagttgaac cagccggcaa tgaaattggt cggaacctcc
    18361 tgcctcttcg cattgtaatc gcggacgaca gcgttgtaat agcgcctggc attctgcagc
    18421 gcgtcttcca cctggttcag actgccctgg agttgattga aagcctcgac ggcgcgcagt
    18481 tgcgggtacg cttcagccag ggcaaacagc gacttcagcg attggctcaa ctgattctcg
    18541 gcgacggcct tggcttccgg gccttgggcc gacatggcct tcgcccgggc agtgatcacg
    18601 gcttcgagcg cgcccttttc gtgcgccgcg taagccttca cggtttcgac aaggttgggg
    18661 atgaggtcgt agcggcgctt gagctggaca tcgatatcag cccaggcgct ttcggccgcg
    18721 gctcggaggc ggaccagcga gttgtacatc ccgatgacga ccagcacgag cgcaaccagc
    18781 acggcgagga tgatccaacc catcggcgtc tccttgcgga tttgggtgaa ggtacgtatg
    18841 gaacttcagg gatgataccg tgacccctgc aatctgtaaa ggatcgcgcg gccttcctgc
    18901 aggggggcat cgcggcttgg ataggctcag atccggtgcc aggcaatccc gtgacggttg
    18961 cgtgactttc cacgcctctc cgggtatcct ccacggcacc ggatggcact cgaacgacta
    19021 tcacgtactc ttgcgcagtt cactcacaaa gatttgagga gggttcgtat ggtgcatcgt
    19081 aacggacgaa tcggcttggg cctggtggtc ctgctgtggt gtgtcttggc tggagggact
    19141 ctcgcggttc cgttgaccag ggctgaggag ccgacgattg agttcaatag tccggacgtg
    19201 ttgcgtcaga ttctggaaca gcaggtcggc aaacgggtga aggtcaggtt gctctcgggt
    19261 caggatgtgg acggcaaggt ggccaaagtc ggcacgcagg cggtagccct gaccgaacta
    19321 tccgggatgg agttcttcga tgcgaccatc cggctggatc aggtggcggc ggtgattgtg
    19381 cggcgcggca agtagtcgcc catcacatgg tcgattacta attctgccat ctaggctaag
    19441 gaaacgatgc ctgtccccat cctacgcaga accgtcgacc tctccggctt tccagacctc
    19501 gtcgtcatct atctcggcat gcgggtcaac cgctggacag ggctgaagac cttattcggg
    19561 ttcggtcctc aaatcgccgc atcagtcgac gctcagccgg agggactgtt gcttcatgaa
    19621 tcactgctct ggtcgctgat gccgccgcac gttggaatgc ggcaatattg gcgcgacttc
    19681 gatgcgctgg aacgctgggc tcgttccgat ccccatcggc agtggtggca gcagtttctg
    19741 cgtgataccg gcggaacagg attctggcat gaggcctatt tcatgcgcgg cgggatggag
    19801 gcggtctaca acgacatcct gcaggacatc gggttcctgc ggttcgcacc gattcggccg
    19861 gctaaggggg cgcagtttac cgccagaagt cgagccggac gaaccggtga atcggcgtta
    19921 ccaccggcgg tgttggaaga ggacctggat cgatgacaac tcaggtgtca ggaggctcgt
    19981 aggccgtcct cacgcgggct tttccgacag gataatcttg acggaagttt cgagggcaaa
    20041 tcgcccgcgc ccacgcggga caagtcgcac atcggggcat ttctgctcca gccgtttccg
    20101 caacagaatc aatctggttt cgaagttatc tttccattca ggcaggccca gccagctatc
    20161 catccgcagc tcgcgattcg tgaactccac tcggtgttca tcctgatgtt gtttcaggat
    20221 ccgccagaga atacgtcccg gcacgttctt gatgaggtat tccccatcga cgaagatgca
    20281 gtcatcagcc tggtagaagc agaacgagcg gtgcgagagc ggtgagtgga gcggcggtgc
    20341 cgctgcggcg ggggcctcga ctgccacgga gtcgtccgct tcctgtgccg actgctcgat
    20401 cccggcggcc agatggccgg ccacaatggc catcaccgcc tcttcgcgaa tcaggagcgt
    20461 gagcggctcc aaggattcag cgaccaacac gccgaggcag cggtgtctga tgatcagcgg
    20521 gaccgcgatc tgtgtggacg cattggagag gccggggaga ggaatttcgc ggcacaggga
    20581 ttctgggccg gagagcgctt gggcacgatc acgtaccgca cgggcataac gaagggtgtg
    20641 gtcgaggccg gaaagccgca gcggcagttt cgccgccgcc accatgccga tgaggccttc
    20701 gccgaaaccg acctcggagc cgaccccgtt ctcgtcatag ccgcggcttg caatggtctg
    20761 gagtgtgcgg cgtttctcat ccacgagtag cagcatcgtg tggtgcaggc cgaagaactc
    20821 atccaggcag cggagtgccg tctccaacag ttcgccgagg cattccgccc ggttcaactg
    20881 ttccgcgacc actctcaagg catgcaggtt cggatcgatg aacagggagg gcggtcgctc
    20941 gtcgtgcgcc gggacaggcc acgaggtgaa gccgtccacc cgctcgatat gacgcacggc
    21001 gtaaatgtcg gcggcgcgca ggcggaagac gtccgacatg ccggcatggg aggcgatggc
    21061 ctgcagttgc agagacatgg cttgaaacaa aggtcccgag gattccgatc gctcataagt
    21121 gagcgaaagc cgataggcct gcatcgcgcg agggtcgagg accatcacgg aggcgaaggg
    21181 atgctcggcg atgttcttgc gcgtcttgtt gaagaactga aacgaaatcg cgacgtgctc
    21241 gggatcgacg taatgcacct ggctgacata gctgatgtta ggcgtgccgt ctttggcgca
    21301 ggtcgcgatg gtggccggga tgatgccttc gaagcagggc cagagatctt tgagtgccag
    21361 cgcggcagtg cccatgcctc acacctgttc gccggcgcca gggccgggag tttgggcgaa
    21421 gacgccggtg acctgcatct cgattgcaac ggcgggctgc atgatgaggg cgtcggtctg
    21481 ctcggccggc acgccgaccg caatgagttc gcccagaaac gcatggcgcc actgctgttg
    21541 tcgctcgtaa tcctgtggag tcgcctgacg gatctgcgtg acgctaccct tgagctggca
    21601 ggtgacgtgg tccgtggggc gtgagcaggt aatcgccatg agcccgttgt cacggaggtt
    21661 ggccagcgta cgggccgatg ctggttccgt gagtaatagc gtcacggtcg tacgattggc
    21721 tgctacccag atgccccagc ctctggtgca ttccggcatg aaattcttat cacgtgtgcc
    21781 gacgttgacg gagacgccgg tctgcagcag atcgacgatg gcaggaggaa tcattgcagg
    21841 cgtagtggca tcacattagg aaaaatttag ggacaccata gcatattctt aggaaggaag
    21901 tcggcgcctc ccgtgatacg ttccctcccg acagtgctgg tgttggaacg cagtccacga
    21961 tcaaggagga tgatcatgaa gagccatata cttctttcac tgtgtctggc cgtgctggcc
    22021 gccacgcctc tcaccggaca ggcgcaggca gcgcagccgg ggaacgtcac gatcaccgcg
    22081 accgactatg gcttcaaaag cccggagcag gtgccgtcag gcctgaccgc catcgaggtg
    22141 atcaacgaag gcgctgaagt ccaccatgcg caacttgtcc ggttggctcc gggcaagacg
    22201 ccggccgact tcgccgcagc aatgaaggcc gatccggagc atccgccgat ctgggccact
    22261 tttgtcggtg gcccgaatgc cgtggttccg ggggatcgcg cgactgccgt gatggacctg
    22321 caaccggggc aacatctggc gctctgtctt gtcccgaaca aggccggcgt ggcgcatgtg
    22381 gcgatgggca tggccaggcc gttcatggtg acggagacca tcgggacccg catcgctgag
    22441 ccgaagcccg atgtggtcat taccgccagg gattttcagt ttgtgttgtc caagcccatc
    22501 accgccgggt cgcacaccat tcaactgctc aacgaaggca gccagactca cgaggtcgtg
    22561 ctggtgaagc tcgcgccggc tgccacggcg aaggacttca tcgcggcgtt cgaacctggc
    22621 gctgtcggcc ctccgccggg gcggccgttg gggggcattg taggaattga acaggggagc
    22681 cggggtctgt tcacggccat gttcgatcca gggccctatg ccttgatctg cttcttcccg
    22741 gataagaaaa ccggcgcgcc gcatttcgcg caggggatga cctgggaatt tgatgtgcgc
    22801 tgatggttca cgcacgaacg ggcggggccg cctcgcggca gttgcatcgg aggccggatg
    22861 gcaccgtata ctatgcccat gcatgacatg atgccgagca ctgttgaacc gatgatggcc
    22921 gatcgtggtg cggcgttgct gcaggtggca gaggtcatcg ctgcgcacca cgatttgccg
    22981 tcgctcttcc aggacctggc gcaatgcctg cctgtggtgg cgccattcga tttcgtcggg
    23041 ctggtcctgc acgatgccgc caagcacgtg atgcaggtgc atgtgctgga aacggcggaa
    23101 gcgaaacggg tgacgcgccg tctcgacggg ctggagattc cgatgaagga ctcggccagc
    23161 ggatgggtgt gggagcatca acaaccgatt ctgatcccgt cgctggcggc cgagtcgcgg
    23221 tttcaagtcg ggatggacgc cttacgggga atcggcgtcc aatcagtctg cttcttcccg
    23281 ctcactaccg cgatgcgccg gctgggcgcc atcggtttcg gctgcctgaa gcccttcgct
    23341 ttcgacgaag ggactgtctc ctttctgaat caggtcgcca aactggtggc ggtcgccgtg
    23401 gacaatgtgc tgcatcacca ggatctcgcc cgcgaccggg accgattgcg cctgctgctc
    23461 gaggtgaccg agtcgatcgc gtcgcatcat gatcagagtc tgttgttggc cgacctggct
    23521 aagcggttgc cccaggtggt gccattcgat ttcatgaatg tcgtccgcca cgacccttcg
    23581 cgtgacgtga tgcggctgtg gctcctggtc acgtctgagc agtccaccat cgaaccggga
    23641 ttggaaacgc ccgtcgacga atcaccgggc ggcctggtgt ggaagacaca agaaccgttg
    23701 acggtggacg acgtcgagcg ggagccacgg tttcccgcct tgatgaccct gtttcgcgcg
    23761 aacggcgtgc gttcgttttg tgtggtgccg ctcaccactg cgcaccggcg tctgggcgcg
    23821 atgggattcg gaagtttgcg gccccgggtc tatctcgaca gcgagatcgc cttcatgaaa
    23881 caggtcgcgc agcaagtggc ggtggcgttg gacaatgcgc tcaactcgga gagcacgctg
    23941 gcctatcagt cgcagttgac cagggaacgc gatcggcaac ggctgttgct cgaggtgaac
    24001 aatgccgtcg tctcgcatct ggatttggat cagctcttcc cggcggtcag cgcctgcctg
    24061 cgccgggtga ttcagcatga cgggtccagc ctgttgctgt gcgatgaaga aaccggccgg
    24121 tggcgcatcc atgtgctgga tttcgatcga aacgaaaatt tcattgaaga ggggcgtatc
    24181 gaggagacca cgcagtcgcc ctcctgcctg gcgattacga ccggtaaagc ggccttgttc
    24241 aatgagcggg atctgctggc catgggaggc gcctcaccct gtgcgcagga tttgctggat
    24301 cgcggcatca agtccttttg ctccgtgccg cttctctcgc gcaatcgttc gatcggcgcg
    24361 ttgaatgttg gacggcggcg ggacgcgggg ttcgatccgg aggacgtgca gttgcttggc
    24421 caggtcgcgc aacaaattgc catcgcggtt gaaaatgccc tggctttccg tgagatcgcc
    24481 gcactgaaag acaagttgac caaggaaaaa gtctatctcg aagaagaaat ccaaacggat
    24541 tacaatttcg aggaaatcgt cggggaaagc cgcgcgctga agcgggtgtt gaaccaggtg
    24601 cagaccgtgg cggccactga ttccacggtg ttgattctcg gtgagacggg gagcggaaag
    24661 gaattgattg cccgcgccct ccacaatctg agcgctcgcc gagaacggac ctttgtgaag
    24721 ctgaattgcg ccgccatccc aaccggcctg ttggaaagcg aactcttcgg gcacgaaaag
    24781 ggcgccttca ccggagccat cgccacgaag atcgggcgat tcgaattggc ggaccggggg
    24841 acgatttttc tcgatgaagt gggggagatc ccgctcgaat tgcaggtcaa gctgctgcgc
    24901 gtgctgcagg agcaggaatt cgagcggctg ggtagcacca ggacgatgcg ggtcaatgtc
    24961 cgggtgatcg ccgcgacgaa tcgcgacctg agtcacatgg tggacgcaca acagtttcgc
    25021 agcgatttgt attaccgctt gaaagtcttc ccggtcaccg taccgccgct tcgcgaacga
    25081 gtcgaagata ttccagtgct ggtgcggcac ttcgtccaga agtttgcctc gcgcatgaaa
    25141 aagcacatcg aaacggtgcc gaccgatgcc atgcgggcgc tgcaagccta tggatggcca
    25201 ggcaacgtgc gtgaactgga aaacttcgtc gaacgggcgg tcatcctgtc atccgggtcg
    25261 gagctgttcg tgccgactgc ggaattaaaa cgtccggtgt ctccgtccaa cgggtcggcc
    25321 acgacgctgg aggatgcgga gcgcgaccac atcatcaaag ccctgcggga cacccactgg
    25381 gtcatcgggg gatcgtccgg cgcggctgcc cgcttgggca tgaagcgcac gacgctccag
    25441 tccaagatgc agaagctcgg catcgtccgt ccccaataac cgaacctccg actcgtcccc
    25501 tttcttctgt ctgtgccgat cagtcgacat ctgccgagat gtcggcagtc actcctcggc
    25561 accggcacct gttttgatca ccgtctcacg gctccttcgt tggaatcgcc tttcgccgca
    25621 acgaattctc gttctcgggc gcggctcctc ggcctggaac aaatcctgct gtgagcctga
    25681 ctgtattgag atcggaagaa ccggaaggag cggctgatgg aatcggtgac tgagttgttg
    25741 gagcagcatg gagcgtgggt gttgttcggt ggtgtgctgg ccgagcaact cggattgccg
    25801 tttccggcat tgccgctgct cgtcgcggcg ggcgtgttgg tggggaccgg tcacctctcc
    25861 tggtccgggg cgttgatcgc cgcgctgtcg gctaccctcc tggcggatat cgtctggttt
    25921 cttgccgggc gctggcgggg ccgtccggtg ctgacgctgt tgtgccggat cgcgctggag
    25981 ccggacgcct gcgtgcggcg gacggaagac tttttccgca agcatggcgt gcaatcgctg
    26041 ttggtcgcga agttcattcc cggattgagc acgatcgcgc cgcccttagc cggaattgtc
    26101 ggcctgggac ttccggtgtt cctgctctac gatgcgctgg gtgctgcagt gtgggcgggg
    26161 tccgggttgg ggttcggcct gctcttcact gaggaggtgg cgttggccct gacctattcg
    26221 gaacaggtgg taccggcact cctgctcgca gcagtggtcc tgctgcccgc ctacatcggc
    26281 tggaaggcct ggcatgttcg ccgccaactt caagccgttc cgaggatgac ggtggcggat
    26341 ctgctggaca agttggcagc gccggatcct cccttgctca tcgatgtgcg gacgcggctt
    26401 gccgttgagg cagagcccgg tcttcctaat gccctgcaga tgtcccttga ggatttggcg
    26461 cggcggcatg gggaacttcc ccgctcccgc gatctggtgc tctattgcgc ttgcccggct
    26521 gatgccgcca gcgcacaggg agtgatgctg ttgcaacgga aaggcttcac gcgggtatgg
    26581 ccgctggccg gtgggcttca tgcgtggcgg gcgatcgtgg acgggcctga accggtgccg
    26641 atgatacagg tggagagact ggcagtctga cgcgcaatac actcacgtcg gcagggaggc
    26701 atctgatgga attcacaatg cccacgacgg agcaggccca ttggggatta cgcgccatga
    26761 aaaccgtggc gctggcggac ggtgtggtcg atgaatcaga gcgacatatg ctggaagccg
    26821 tacagcgcgt gttcggcaca catcatgacg tggacggcct ccaaccactt gcgccggagg
    26881 agttggcggc agggttgccc gatcagcaga tccgccggca actcgtcaac ggcctgatcg
    26941 tgatgtcctt gatcgaccag gacgtgaagc gtgcagaggc ggaactcgtg gagcggtttg
    27001 cctcggcact ccaggtcacc ctgcctgagg tgacgaatct ccgccatgtt gtgaagaagg
    27061 aactattcca cctccgcctc gatttggttc gccgttcgat gatcgcgccg ttatttcggc
    27121 agatgccgac gggaaggcag tcggaattcg gcacgcgaca gcatccggcg ctgccgagaa
    27181 agaagtggcg tgaattatgg cagaagaatc ggtggttcac ggccatggtg tagacgggtt
    27241 cggcaggaac gcgatttgct gtgatcagcc agcgttgctt ctgttgatca agtgagtctg
    27301 tcgccagttt catctgtcaa tggtcgggag gaatggtcat gaatcgtcgt ggatggatcg
    27361 gatcgtcgtt gttgatgctg acggtgatcg gaatcggggt cgggttggcg gtgtggaaat
    27421 atggcagcat ccaggatcaa gccgctgcgt cgaccaatca gccggaaccg atggaagtcg
    27481 tcacgctcgc agtggcccgg gccgttgacc atcaccagac tgcgacctcg atcgggaccg
    27541 tgttggccct ccgctccatt accctccgca atgaattagc cgggacggtc agccaggtgc
    27601 ggttcacgcc ggggcagatt gtcgaagccg gcacggtgct ggttgccctc gatgtgtcgg
    27661 tggaagaagc cgatcttcgg gcacagcagg cccaggcggt gttggcccgt actgtcctcg
    27721 atcgacgccg ggcgctgaca caggatcttg ctgccgcgca ggaagaggtt gatcgcgccg
    27781 gggcggatct cgatgtcgtg caggcccaaa tcgcacgcac caaagcgtta attgcgcgca
    27841 agaccatccg cgctccgttc cgtgcccggg tcggcctcgc cgacgtgcac cccggacaat
    27901 atctgaatga aggcacgcag ctcacgacgc tacaaggcat cgatgaagcc gtgcatgtgg
    27961 actttgccgt gccgcagcat gtggcggccg gattgcgaca cggcgacctg gtggatgtgt
    28021 atccggcggg tgacacgtct gtgcttcagg ccaagatcgt ggcgttggat gcccgcatcg
    28081 atcccgcgac acgcaatgcc atggtgcgcg caagaatcga tcggtcaccc tcgatgccga
    28141 caccgggcgc ggccgtgcgg atcaaggtgc gggtcggtgc gccgcgcagc gcggtggcga
    28201 ttccggtgaa tgcggtgcgc aaggggccgg gaggcgatca ggtatttgtg atcaattctg
    28261 acggtcaagg caaatcgcgc gcgcaggtgc gtcacgtgga gagtggtgcg atgtcgggtg
    28321 atgacatcat tgtctatgcc gggttggccg ccggagaaca ggtggcggca tccggtgcgt
    28381 ttaaattgcg tgatggcgtg ttggtggtgg cggccggtgg gccggtcgcc aagtccgacg
    28441 caactccttc gccggagcaa ccgtaaatca gagaggcatg accatgcgtt cattcactga
    28501 tgtcttcatc aagcacccgg tcttggccgt ggtgatcaat ctggtcatcg tcctcgtcgg
    28561 ttggcgcgcg ctgacgacgc ttccggtgca gcaatatcct aagatcgaaa gttcgtcggt
    28621 catcatcacc accgtctatt acggggccag cgccgagacg gtgcgcggct ttctgaccac
    28681 gccgattgaa cgggtcgtgt cggccatcag cggcgtcgac tatctggaat ccaccagccg
    28741 ggcgggcatc agcaccgtca cggtgcacct caagttgaac cacagcagca cggcggcgct
    28801 ggcagaagtc acggcacgcc tgcaacaggt tcgctctgag ctgccggcgg aggcggaacc
    28861 accagcggtg gaggtgcagc gggccgatcg tccttatgcc tcgttttatc tgagcttcac
    28921 gtcaccggaa cgcacggtgc cggccttgac tgattggttg ctgcgatcgc tccagccgca
    28981 atttgccacc ctgaccggtg tgcagcgcgt gacgatcgag ggcggacggc agatcgccat
    29041 gcgggtctgg atcgatccgg accgtctcgc cgcctacaac ttgtcgcccg gcgacgtgca
    29101 agcggcgttg cggcgcaaca actacctggc ggctgtcggc cggacgaagg gcaacctggt
    29161 gcagatcaat ttgctggcga atacggatct gcgcagcacc gaagagttcg aagacctcat
    29221 cgtcacggac cgcggcggag ccatcgtgcg tttgagagat gtggcgcgag tggagaacgg
    29281 cgctgaggaa gctgaaatgg tcgccaagta taaccgcacg gaaggggtgt atctcggtat
    29341 ctggcccctg gtgggttcga acgaaatcga ggtggcgcac cggttgcggg aggaaatgga
    29401 ccgcattcgc cccacgctgc cgaaggatat cgatatgcag ttggtgtggg acggcaccat
    29461 gttcatgcgg agcgcattgg aggaaatcac caagacactc ggtgaaacgg tgctgatcgt
    29521 ggccacggtg gtgtttcttt tcatgggttc ggtgcggaca gcgttggtgc cgctggtcgc
    29581 catgccggtg tcgttggtcg gcgcggcgat cttcatgtac gcattcggct tcagcctgaa
    29641 cctgttgacg atcctcgcta ttgtgttgtc ggtcggattg gtcgtggacg atgcgatcgt
    29701 cgttgtcgaa aacgttgaac gtcatgtgcg cgaggggaaa tcccgaatac aggcggcgct
    29761 gctcggttcg cgtgaactgg tcgggcccat catcgcgatg acgatcacgc tggcggcggt
    29821 gtatgccccg atcggctttc aaggtggttt gaccgggtcg ctgtttttgg aattcgcgat
    29881 gacgctggcc gccgccgtgg tgttgtccgg cgtggtggcc atcacactct cgccggtgat
    29941 gagctcgtat tttgttcatc ctgaaggtaa ggaaggtcgg ttgaccaaga tggtgaatcg
    30001 ggtcttcgac gccacgcgcg caggctatgg acgtctgctt gatggggcct tggagatgcg
    30061 ctgggcgatt gtggtcgtgg ccctcttggt gatggtcgcg gcctggccgc tgtatcagtt
    30121 ctcccggcag gagctggcgc cggtggaaga tcagagtcac atcagcctgt ttctggaagc
    30181 cgcgccggat tcgacggtgg cggccacgaa tcgggattct ctcaaagtga tcgatgcgct
    30241 ccgagcgttt ccggaggcgc gcttcacctg gtcgctcacg tcatcgtggg gcgggttcgg
    30301 tggcttggtc gccaaagatt ggcgcgaacg gtctcggtcg acggagatga tgtatggcca
    30361 ggtctatggc gcggtgtcgc aaattcctgg gttgcgcgtc tttccgcggc tcgatccgcc
    30421 cctgccgacg ccggggcagt atgacgtgga attgattctg gaaagtcagt tgcctgccga
    30481 gcagttgatg gaaacaacgg cggccgtgct cggtgccgga tggcagagcg gaaaatttct
    30541 gtatgtcgat acggacctca agctcgattt gccagaggcg cgcgtgctca tcgatcgtga
    30601 gcggctggcc gacctggggt tcgacctggc ccgtgtcggg caggaactgg gcacgctgct
    30661 cggtggcgcc tacgtgaacc gcttcaattt ctatgaccgg agctacaagg tcattccgca
    30721 gatcggcgac aaggatcgag ccacgctgga tccgctgctg gacttgaaga tcaagacgcc
    30781 cggggggcag ctggtcccgg tgtcgacctt cacccgcatc gagaccagca cggcccctcg
    30841 gacgctgaat cggtttcagc aacggaatgc ggtccgcatc ttcggcggag tgaagccggg
    30901 cgtgacgaag gacgaagcgt tgcgtgtgct ggaaacggct gccgcggccg ccgccggttc
    30961 tcgcgcgact ctcgattatg cgggcgaatc gcgccagatc cggcatgagg ggtcggccct
    31021 catcgtgacg ctgggattcg ccgtggtgct gatctatctg gtgctggctg ctcagttcca
    31081 gagttttcgt gatccgttga tcgtgctgtt gggctcggtg ccgttggcta tctccggcgc
    31141 gctcgtcttt agtttcctga acctcacgac gatcaacatc tattcgcagg tcggtttgat
    31201 cacgctggtg ggcctcattg cgaagaacgg gattctgatc gtggaattcg ccaacacctt
    31261 gcaggcgcgt ggcctgtcga agacggcggc gttgcgtgag gcggccatga cgcggttgcg
    31321 ccccgtgttg atgacctcgg cggcgacggt gttcggccat ctgccgctgg tgctggtgtc
    31381 ggggccgggc gcggcagcgc ggaacagcat cggcatggtg ctggtcacgg gcatgacggt
    31441 cggcacgctg tttacgctgt tcgtggtgcc ggtgttctat tcgttgatcg ccacgcgcca
    31501 ccaaccggcc gaggagttgg aaccggccga ggaaccgggt ctgcaactcg cgggggcaaa
    31561 ggggtgaatg gactcattat gttgaaaatt acatcggagc agactggaga ttccgcccgg
    31621 ctgaccctgg agggcagttt gagcgggccc tgggtgacgg aattggagcg aacctggcag
    31681 accgtgcggc tgtccggcat atttgcgccc gtcgtagaac tggtcgggat cacgttcatc
    31741 tcggaagacg gcaaggccct cctggccagg atgtggcggg aaggggcggc cttggtcgcc
    31801 catggctgct gtaccaggca cattgtcggt gaaattaccg gagcagcccc ggtggcgggc
    31861 cccggccagt gcgaccctgc atagaatagg ttcatcatgc gtatgattgc gtgtgtcatc
    31921 actctgctcc tcgtgaccgg ctgtgccgtc ggtcctgact attcacgtcc cgaccttcag
    31981 atgccgaccg ggttccggat ggcggtttct gatcaggaaa cacagtcctt tgccaatctt
    32041 ccctggtggg atctgttgca ggacgaagaa ctgcgccggc tcattcgcgt cgcgctggcg
    32101 gagaacaagg atctgcagcg cgcagtggcg tccgtggaag aattccagtc gcgcctgctc
    32161 atctcgaaga tggatttcgc tccgaaggcc gatgtcacgg ccaatgcgcc gtcgttcggc
    32221 cgtaaggcca atttcctgtt tccaggattt cccaatccgt tcaattacta tctccaagga
    32281 aatctgtcct gggaattgga tatctgggga cgggttcggc ggtcgaacga agccgcgaga
    32341 ggagatctgc tcgcccgcga agaagcccgg cgtgcggtgg tgctgcagtt ggtgagcggg
    32401 gtggcggagg cctactttga attgttgcag tttgacatgc agctgggtgt ggcgcagcgc
    32461 accttgaagt cgtgggagga atcggtccgg attgccgagg cccgattgcg tcaggggatg
    32521 atttcacgta tcgacgccga tcagttcgag gcggagcgcg cgaatgcggc cgctcgcgcg
    32581 gcggaattga ccaggcagat ggtgcagaag gagaaccagc tcagcatcct gcttgggcga
    32641 ccgccggggc aagtggcgcg gggccgttct ttgacggagc aggtgttgcc gccggaggtg
    32701 ccggccggtc tgccgtccga attgctccag cgccggcccg acctggtgca agccgaacag
    32761 gaattggcgg cggccacggc gaggatcggg gtcgccaagg cggatcgatt cccgaagc
//
LOCUS       DAOQDS010000046        32372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_474_length_32372_cov_21.083516, whole genome shotgun sequence.
ACCESSION   DAOQDS010000046 DAOQDS010000000
VERSION     DAOQDS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 32372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32372
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_474_length_32372_cov_21.083516"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PK589_12305"
     CDS             <1..291
                     /locus_tag="PK589_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNJ20434.1"
                     /translation="PELSRSDAEGLQGNSLETIRQMVASGLGITVMPCSAVTSRHLNK
                     RLTVLEFAKPVPERRIALAWRKGFARPAVIAVIHEAVGMLKIPGLESISAQA"
     gene            315..668
                     /gene="uraH"
                     /locus_tag="PK589_12310"
     CDS             315..668
                     /gene="uraH"
                     /locus_tag="PK589_12310"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HNJ20435.1"
                     /translation="MEDAHRISIHVLDQSQGRPAEGIPVTLEIRGLTGLWKQLGQGRT
                     DADGRSSDLYPAGLRMQTGTYRLTFDVAGYFRKRNVATFFPEVPIVFTVQDVAQSYHI
                     PLLLSPFGYSTYRGS"
     gene            complement(687..1364)
                     /locus_tag="PK589_12315"
     CDS             complement(687..1364)
                     /locus_tag="PK589_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNJ20436.1"
                     /translation="MKVMLASMCAGLVMVVLGMAVTASPAPDKDQLLIAAAERNDVES
                     VKRLLRDGAGVQARDAQGRTGLMAATYHNHVDVARVLIEAGSDVNAQDAMHNSPLLLA
                     GASGYLDIVRMTLAARPDFTVYNRFGGTALIPACERGHVEVVKALLKTTIDVNHVNRL
                     GWTALLEAILLSDGGTAHQEIVRLLLAAGANPNLADHDGVTPLRHARQKGYRVISDLL
                     LAAGAHE"
     gene            complement(1361..2239)
                     /locus_tag="PK589_12320"
     CDS             complement(1361..2239)
                     /locus_tag="PK589_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ20437.1"
                     /translation="MADPHKRLDANIPGNFYVDDTCINCDACRQLAPASFEEVGDYSA
                     VRHQPASAQEIREAYRALLACPTGSIGTEQGDKGVMQAAKADFPLLVEDHVYYCGFNS
                     EKSFGANSFFIRHPEGNWLIDSPRYLKPLVEAFDRMGGIAHIFLTHEDDVADAARYAQ
                     RFGGRRIIHRADAHVMPEAEEIVEGLDAMFYRPEFLIIPVPGHTAGSLCLLYRDRFLF
                     TGDHLWWDPETNELGSPQQLVWDADALRRSIATLTSYPFEWVLAGHGGRIHLPHAHMQ
                     MALRALVRRREGTRLS"
     gene            complement(2223..2906)
                     /locus_tag="PK589_12325"
     CDS             complement(2223..2906)
                     /locus_tag="PK589_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNJ20438.1"
                     /translation="MSEPSAEIAHLSKIDPVMRRVIDAVGPYALPPPPRRSPFEALVR
                     AIAYQQLHEKAAECILTRFIALCPGRRFPQPADVLALAPESMREAGFSRAKIAALQDL
                     AAKAIDGTLPTAAVIRRLDDDAIVDRLVAVRGIGRWTVEMLLIFQLGRPDVLPVDDFG
                     VRNGFRIAYKRPMPTPQELLRYGERWRPYRTAAAWYLWRAVDQAKALQAVTALSKPST
                     RRVSNGRSA"
     gene            complement(2914..3297)
                     /locus_tag="PK589_12330"
     CDS             complement(2914..3297)
                     /locus_tag="PK589_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNJ20439.1"
                     /translation="MGRQNVSTGGPWEGKIGYSRAVRVGQSVQVSGTTAMTGAGLVGK
                     NDPYAQTIQALKTIEAALVQAGASISDVVRTRIYMANIDQWQEVGRAHGEVFGSVRPA
                     TTMVEVKRLIDPEMLVEIEADAIIG"
     gene            complement(3475..4206)
                     /locus_tag="PK589_12335"
     CDS             complement(3475..4206)
                     /locus_tag="PK589_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ20440.1"
                     /translation="MRPLTGKVAIVTGSSSGIGRAIAERLARDGATVVVHYHVHAEKA
                     NQVAEGIQSSGGSALVRQADMSRVADAQRLVRETAKELGRLDILVNNAGRFLPRPLLE
                     TTEEEFDAILALNAKGPYFAMQAAARVLSDGGRIVNISSALTHLKFPGATAHLGSKAA
                     LEQYGKGLAQELASRGITVNTVLPGFTDTGVLTEPYRQMGAQMSPTQRLGHPSDIADV
                     VAFLVSEQARWLTGQVIHAGGGLVM"
     assembly_gap    4269..4368
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4637..5242)
                     /locus_tag="PK589_12340"
     CDS             complement(4637..5242)
                     /locus_tag="PK589_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNJ20441.1"
                     /translation="MEKPAETQYPIHDVLRQRWSPRAYSERMVEPEKLRSLFEAARWA
                     PSSNNEQPWHFIVGTKADPAAYDRLFACLKEGNKKWAFRAPVLMLSVARLNFEDEGTP
                     NRHALHDTGMAALSLCLQATAMGLIAHQMAGFDMEKARTDLGLPAGHEPVAMIAVGYP
                     GDAAELPDYLREREFKPRERKLSTEFVSHGTWNDQPGWLKP"
     gene            complement(5261..5845)
                     /locus_tag="PK589_12345"
     CDS             complement(5261..5845)
                     /locus_tag="PK589_12345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HNJ20442.1"
                     /translation="MTEQRDKRNMLAGELYRASSAELTMERRRAQMMLARYNAITDGE
                     PDLLLSLLRDLMGSVGDGTVIMPQFTCDYGYNIRLGRNVFINYHCIFLDCAPIDIGND
                     VQVGPAVQLYTAQHPLEAEIRRSGLESARPIRIGSDVWIGGGAVILPGVTIGDRSVVG
                     AGSVVVHSVPADCVAVGNPARVVRTLGSPTLTTE"
     gene            complement(5863..6561)
                     /locus_tag="PK589_12350"
     CDS             complement(5863..6561)
                     /locus_tag="PK589_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNJ20443.1"
                     /translation="MAMVHEHDGLAFLFDLDGTLVDSVYQHVLAWREATQAAGIELPV
                     WRIHRQIGMSGGLMLHALLRETGRPVSKEEAERIQRVHAEAFVRQAPSLRILPGAQAL
                     LDALTAGRVPYAIATSGRLQSAQHTLKLLKLPEGTPVVTRDQVKRAKPDPDLFLAAAE
                     QLKVPIAKCIVVGDSVWDLLAARRASALGVGLLSGGYGREELERAGAYRTYHDPADLL
                     RHLDECGVRPDPLM"
     gene            complement(6567..6992)
                     /locus_tag="PK589_12355"
     CDS             complement(6567..6992)
                     /locus_tag="PK589_12355"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNJ20444.1"
                     /translation="MKRNASAKWQGDLKTGKGTVSTESGVLAGTQYSFSTRFENGKGT
                     NPEELIAAAHAGCFTMALSGQLGAANLVADQLATTATVTMEKLEAGWTVTGIHLDVKG
                     KVPKADNAAWEKATTAAKTGCPISRLLNTTITMDAKLES"
     gene            complement(7007..8017)
                     /locus_tag="PK589_12360"
     CDS             complement(7007..8017)
                     /locus_tag="PK589_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HNJ20445.1"
                     /translation="MKAVQATAPGAAWELVERDIPRPKPDQVRVKVQACGICHSDMFV
                     KEGHWPGLQFPRIPGHEIAGLVDEVGSAVTAWKPGQRVGVGWHGGHCGQCDACRRGDF
                     ILCRSGQVCGISYDGGWAEYVVVPEQAMAALPDELSFEEAAPLLCAGITTFNSLRHSG
                     ARAGDLVAVQGIGGLGHLGIQYASKLGLKTVAVGRGKDKEPLARQLGAAVYLDAAVVN
                     PAEELQRLGGARVVLATAPDSKAIASMVDGLGANGTLLIVAAPGEPVMVNAITLIGKR
                     ASVQGWPSGTAKDSEDTLRFSAMTGVRPMIERYPLARAPEAYQQMMSGKARFRAVLTM
                     NS"
     gene            complement(8160..8531)
                     /locus_tag="PK589_12365"
     CDS             complement(8160..8531)
                     /locus_tag="PK589_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNJ20446.1"
                     /translation="MATPVMAHDSAKAKEFFEAKMAFTTGPVELERMMKNKEVNVVDV
                     RAAEDYAEGHIPGAVSLPKDQWAGLKGLRKDKVNVLYCYSQVCHLAATAAVQFAGQGY
                     PIMELEGGWRWWKDDGFDVEK"
     gene            complement(8692..9846)
                     /locus_tag="PK589_12370"
     CDS             complement(8692..9846)
                     /locus_tag="PK589_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ20447.1"
                     /translation="MTAGQRAARWTQRLAALTWKELLQLSRDVPLLLFLIYSFSLSVL
                     VSGVGITMQLTNAELLVHDEDQSPSSRELIHRFQPPYFAVVGTINNPRQGLQQLDQGQ
                     AMVVLDVPPRFHEALVSGERTAVQLLVDTTNAPQGLSAAAYTVRIAVPFGTETALASA
                     GLSGTKMSMPLVSSAHRVWFNPTQDERWFQSISHLLRMITLFAVLLPAAALVREKERG
                     TVEQLLVSPLSPFQIMFSKVLAMAGVILLATALALYGVLHPVFHVPMKGSALLFFLLT
                     ALHVFTTAGFGLMAATLAKNQAQVAMMALFLVGPMLLLSGITSPFESMPRWVQAIMTL
                     SPLRYYIDITYGVMLKGAGLDLLWKPVGAMLLLGGALFGSGMWRFRRQFQ"
     gene            complement(9857..10987)
                     /locus_tag="PK589_12375"
     CDS             complement(9857..10987)
                     /locus_tag="PK589_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ20448.1"
                     /translation="MALKEWKETTRDRLFLLLAFLLPALWLVVFGYGLNLDVEDIPFA
                     VLDRDQSEFSREYLQRFIQSRYFSFRGYAHDEHSLDRLLTETKIRAAIIVPERFQEQL
                     VAGEAVAVQTLLDGTFPLHTDIAKGYVIAINQAFTEDRLIDFLRRSRGLTREQAGVLV
                     RPLSVEVRYLYNEEVRSTWSMVPALVMFTLMLASPLLTALGVVREKETGSIYNIYSST
                     VSRAEFLTGKLLPYILISLVNVCVLWLMAVGLFQVPFKGHFLLFFFASVLFVCCTTGI
                     GLLISLLVQTQMAALIITMVVAMIPTILFSGLLVPVASLTRGAKVQAHLFPAMYFTNI
                     VRGSFLKGVGVEVLWQDLLALALFAGGLGGLTYHLFTKRPKA"
     gene            complement(11008..12987)
                     /locus_tag="PK589_12380"
     CDS             complement(11008..12987)
                     /locus_tag="PK589_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ20449.1"
                     /translation="MMGPPASSADEVVRVSGFVKRYKQHRAVNGLDFTVHRGEIYGLI
                     GPDGAGKSSVMKAIAGVLGYEEGSVDVFGTLLDSERAAEQVKRRIGFLPQGLGLNLYP
                     ELSVEENIDFFAKLRLVPETDLQARKARLLSITRLDRFRSRLMKQLSGGMKQKLGLIC
                     TLIHEPELAILDEPTTGVDPVSRRDFWAILAEWQAVKGMTALVSTAYMDEAVRFHRLS
                     FLSHGRVLASGTPADVQSLVPGVVVTFESTPQLEAVERLKHRYAQVESLGPSLHVFSP
                     EQDAEAAVAGIRQVLDPLPVAHLRTDEPELEDVVVALLLKEQDGQTRASERSSQSAPP
                     ANRPQFDGPAVEAKELIRDFGSFRAVDRVSFDMQQGEIFGLLGANGAGKTTVIKMLTG
                     IVPPSGGEGRVAGADMRTASGAIKERIGYMSQAFSLYLDLTVIENIRLFADIYGLARR
                     QAQQRMAWIVEMAGLSGYEHDRTGRLPMGVRQRLALGCALVHSPRVLFLDEPTSGVDP
                     IGRRRFWDLLTRLAREEGVAILITTHYLSEAEHCDRLALMYAGHIVAEGTPADLKTQV
                     EHEVGQLLEIAVDKPGAALAQVTAAGFTGAALFGTKIHVLSRDPSRDEARLREVLGRA
                     DTTVAAVRPRMLSLEDVFVSRVMALEQAAQKKGAS"
     gene            complement(12984..14087)
                     /locus_tag="PK589_12385"
     CDS             complement(12984..14087)
                     /locus_tag="PK589_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ20450.1"
                     /translation="MTIRTSMTAAAVLAVVVIAGYVLLDRLVWHNRLPDGLIQANGRI
                     EGDHVTVASKFPGRVVELAAREGAEVLKGSILIRLDDAQTKAKVDQAARLVDSLMAQV
                     EAAHTALAVLNLEVPLSIEAAAAKVESARAVIRKARAVEYEARRDAERIRALLPQQAV
                     SEQQVDQADARWKIAVTEIAVAQAAHDQAGKELAQAELGRERIRSKEAEVAALERQRD
                     QADATLAEARSVLDDLTIVAPTSGTVTTRMVDVGEVVATGAPLLELVDLDRLYLQVYV
                     PELQIGKVRLDLPARIHTDAFPDQPFDATVRYIASKAEFTPKEVQTPDERVKLIYAVR
                     LYLTTNPDHRLTPGLPADAVIRWKDDVAWSKPR"
     gene            complement(14229..14669)
                     /locus_tag="PK589_12390"
     CDS             complement(14229..14669)
                     /locus_tag="PK589_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNJ20451.1"
                     /translation="MKMQQTVRVAIGVLVLAGALVGAGCHRHHTPAERADWMTNKIAK
                     HLDLDDQQKAKLMAVRDEMVAARAESQAEHKAIMEAAIAQVQSDRLDQAQLIQLMDRH
                     QAEQKRVMQRVLPKLTEWHATLRPEQKAEAVEHIRKWMDRYGDH"
     gene            complement(14727..15023)
                     /locus_tag="PK589_12395"
     CDS             complement(14727..15023)
                     /locus_tag="PK589_12395"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNJ20452.1"
                     /translation="MPVTLINVFSVPAATETEFVQWWQDVKAHITTQAGFISGTFHKS
                     LKPDSRFNYINVAIWENETLYWKAYEKSVTPMKEKLGALGVDMTPALYHVAFEY"
     gene            complement(15144..16061)
                     /locus_tag="PK589_12400"
     CDS             complement(15144..16061)
                     /locus_tag="PK589_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20453.1"
                     /translation="MTRTMIVLGSLVLSVALGASSGFTAEGGGKSATHMKVSGVVSKV
                     QSGITTVKTPWGTMKIASTLTPKNLEVGEDVEMQVNENNAVIDVHRKGDKAHSHRFVT
                     GNLAYTAPDRREIKVWTPEGEKAIDVQTGRSKLSALEEGAPVTVELNEAGKMIDINRF
                     TVEMSFDEHPRTKPGYVIQVNGTVTKIQSGLVYVKTPAGQYTISANTAPADAAVGDDV
                     SLWINEEGMVIDHHGKDKHKAGSHRLIFGKLIYAGTTKNVIKLATPEGVKEFPLERME
                     VKTKPIAEGSHIVVELNEEGTVIDLRKAQ"
     gene            complement(16075..16359)
                     /locus_tag="PK589_12405"
     CDS             complement(16075..16359)
                     /locus_tag="PK589_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20454.1"
                     /translation="MLKITLSNTSTGTTFVLSGRLAGPWVDELRTCWVKQEIDQPAPC
                     HIDLREVTFIDEAGTTLLSQMQRAGVTIEASGCLTRAIVQRLDKVVLSQE"
     gene            complement(16424..16930)
                     /locus_tag="PK589_12410"
     CDS             complement(16424..16930)
                     /locus_tag="PK589_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNJ20455.1"
                     /translation="MYSFFKTDDSWAGLILRVVLGGVIFAHGAQKLLGWYGGFGFEGT
                     MGFFTQKMGFPWLVAFLVIIGESIGSLGLIAGLLTRFTAASFVVIMLGAIVTVHLPQG
                     FFMNWFGQQTGEGFEYHLLVIGMSLALLIIGGGKWSLDGLIARWFGERAALPVRKPRE
                     ASFALRMF"
     gene            complement(16952..17083)
                     /locus_tag="PK589_12415"
     CDS             complement(16952..17083)
                     /locus_tag="PK589_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20456.1"
                     /translation="MKDITLWVGWYGMLLTLPLFVAGLLLKDRTETAVLQWVRSHRR"
     gene            complement(17283..19349)
                     /locus_tag="PK589_12420"
     CDS             complement(17283..19349)
                     /locus_tag="PK589_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ20457.1"
                     /translation="MSDRDSSGIERYRALLRVAEAIASHSDLSALVQDLARLLPLVVP
                     VNFVGLSLYDSQRGVMRLQVLRANVPAEIIGGHESDPQDTPAGLVWETQQPLLLNDLP
                     TERRWPKVIELMCEDGVRSCCLVPLTSPTQRLGSLEFSSLEQSAYSTSDVELMQQVGR
                     QVAVAVENVLNREAAAASQRDVERQRDRFSLLLRMTNSMASTLDLRQVFKAVSLCLRE
                     LVPQEYASLILCDGQTQRVRLHALDFPDNEGALTEGALSDVAGSLAGLAFERRRPAVA
                     NTLADLKAFSHAVPQLLVRKGFRSMCSLPLLSRDRVIGCLNLASHHEQAFGEQDLEFL
                     SQVAGQIALAVDNAVAYQEIHTLKDRLTEEKLYLEEEIRVEHGFDDIIGDSRPLKQIL
                     GQVEIVAPTDATVLILGETGTGKELIARAIHRLSGRHARTFVKLNCAAIPTGLLESEL
                     FGHERGAFTGAIAQKVGRFELAHGGTIFLDEVGEIPLELQSKLLRVLQEQEFERLGST
                     RTIRVDIRLVAATNRDLAKLVEEGRFRGDLYYRLNVFPLNLPALRDRREDIPMLVRYF
                     AQHYAARMKKTIESIPAPTLEALSRYHWPGNIRELENLVERAVILTPGTQLQVPLQEL
                     KIEARQAPAPGATLQDAERDQILRTLRDTRWVIGGPDGAAARLGLKRTTLTSKIKKLG
                     LSRPRE"
     gene            complement(19411..20151)
                     /locus_tag="PK589_12425"
     CDS             complement(19411..20151)
                     /locus_tag="PK589_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ20458.1"
                     /translation="MSDAMDDSGFPVKLIERRLIAEGTMAFHFAKPSQFVFTPGQFVD
                     LTLLQPSETDAAGNSRAFSIASAPHEDTIMVATRLRDTAFKRELQRMPIGSTVRMEGP
                     FGKLVLHSDQTRPAVLVAGGIGITPFRSMVVHAAQQQSPQRLVLFYSNRRPEDAAFLD
                     ELQSLQDKNRHYIFVGTMTEPEKSLHAWRGETGYLNAGLLSKYLAEIDKPMYYVVGPP
                     GMVTALRTILKDAHVEDTDIRIEKFAGY"
     gene            complement(20195..20431)
                     /locus_tag="PK589_12430"
     CDS             complement(20195..20431)
                     /locus_tag="PK589_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNJ20459.1"
                     /translation="MEEPVIAAKQPAVLELEAGTYYWCRCGRSKKQPFCDGSHKGTSF
                     TPMEFKTVEKKTLALCQCKHTKNPPFCDGSHKSL"
     gene            complement(20662..21108)
                     /locus_tag="PK589_12435"
     CDS             complement(20662..21108)
                     /locus_tag="PK589_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ20460.1"
                     /translation="MKAHYLGHVVFYVKDLQRALAFYRDLLGFHEVGRIFNGAAAALT
                     SGRTHHELLLIQVGDAPGPPPGRRRGLYHIGIKVGDSLDELRTAKRELEQAGIAIDGM
                     SDHTVSQSLYLRDPDGNEVELYVDADEAIWKTNPDAVVSPIKPLVL"
     gene            complement(21138..21530)
                     /locus_tag="PK589_12440"
     CDS             complement(21138..21530)
                     /locus_tag="PK589_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20461.1"
                     /translation="MSGQDHTTAHLDNVSRALLRLHKALLDAERVSYERVHGRIPTNG
                     AFFQLVLGDAWFAWLRPLSQLMARLDELSEEKEASDQGEITAVIGSVRTLLTPSEEGN
                     GFGRNYYDALQREPDVVLAHAAVRALLR"
     gene            complement(21560..>22449)
                     /locus_tag="PK589_12445"
     CDS             complement(21560..>22449)
                     /locus_tag="PK589_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNJ20462.1"
                     /translation="SGICNHIGGPLGEGRLEGDYVVCPWRLYVDLTSATKRRKKAKVP
                     HPLARPIVRAEGPIRVLGIATTAMTTDEPRYSASDALLEAALEHAREHLQLDTQLIRL
                     RDLSFRPCEGFYSKAAEACTWPCSITQMDAADQMDRVYEGIVHWADVILVSTPIRWGG
                     ASSLYYKMVERLNCIQNQETIANRHLLKDKVAAFIIMGGQDNVQGVAGQLMTFFAEVG
                     CQFPQFPFIDHSRGWSAEDMERNNREVQGSRELREGAEALVTRAADLARLMLEARLIS
                     APLARGGRKAHQLDSEPTG"
     assembly_gap    22450..22549
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22550..22728)
                     /locus_tag="PK589_12450"
     CDS             complement(<22550..22728)
                     /locus_tag="PK589_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNJ20463.1"
                     /translation="MPDVRWTEIGLVEELKGKPLQEVVCGNTKLALSYRDGTFAAISG
                     ICNHIGGPLGEGRLEG"
     gene            complement(22766..23224)
                     /locus_tag="PK589_12455"
     CDS             complement(22766..23224)
                     /locus_tag="PK589_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNJ20464.1"
                     /translation="MKNLEQVRTLAKDLTKTYPRSPREMLGGYVIGARCADKCRAFLL
                     GINGEYNYWPCSLAALLFTFTGISPDQFKEVVATGATDEELGDWLKEHSKVQDRQAII
                     EWNNTMRDLRLSEMAPAVQAYMEDYIPKYVPSHRPVYTYFDVYDLEEQRL"
     gene            complement(23221..23667)
                     /locus_tag="PK589_12460"
     CDS             complement(23221..23667)
                     /locus_tag="PK589_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ20465.1"
                     /translation="MSVQVYGCNHIVIEVTDAKKAVKFYSDVFGLEMLRGGEGAAWCK
                     LGEHQFLAIFEVKTLQPDRMKHFGLMVRDADQIKEVRRKLTKKYKLKMEPHFRCDFRD
                     PWGNRIQVGDLSDESLVWLLPYQEVQKAGITFAKPSRAGSGKGATT"
     gene            complement(23680..24321)
                     /locus_tag="PK589_12465"
     CDS             complement(23680..24321)
                     /locus_tag="PK589_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNJ20466.1"
                     /translation="MTAPVLTIEVYSDVVCPWCYVGKRRLEQALESTGRQDSALVFWR
                     PFQLNPTMPKAGMDRRVYLEAKFGGPEEMQAIQNRIAGAGVSAGIEFAFDRIARTPNT
                     FEAHRLIWFAQQQGRQDAAMEELFHGYFTDGLNIGADEVLVSLAARAGLDGAAVGRFL
                     QSQDGVEAVRQEEARGRQLGIRGVPYFLLNGKTVVSGAQPAETFLQAIEHTTR"
     gene            complement(24318..25202)
                     /locus_tag="PK589_12470"
     CDS             complement(24318..25202)
                     /locus_tag="PK589_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNJ20467.1"
                     /translation="MRTETMNAKELVGVYQAGSHHMVGDGFPVRNMIPGAGVEEQLSP
                     FLMLDYMGPEEFPPTTRRLGVGEHPHRGFETVTIMYHGKVAHRDSTGSGGVIGPGDVQ
                     WMTAASGVVHEELHEKEFAEKGGLLEGVQLWVNLPKAFKMTAPRYQTLLSSDIPMVEL
                     GGGAGRLRVIAGEFRGVAGPAKTFSPVHLYDLRLAAGHRLELDLPEGFNSSLFVLHGQ
                     VVVNGSQSVDDVHIALFGQRGERVVLEAKQEATILVLSGKPIAEPIARYGPFVMNTRD
                     EIIQAVQDYQAGKMGHLS"
     gene            complement(25217..26074)
                     /locus_tag="PK589_12475"
     CDS             complement(25217..26074)
                     /locus_tag="PK589_12475"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNJ20468.1"
                     /translation="MLVVLGATGHTGSVVAQTLLTHKQPVRVVVRSADHGATWAAKGA
                     EVAVASLDDQAALTKALQGASGVYLLVPPNYGATSWLAEQRRRVDQTAEAVKATRIAH
                     VVLLSSIGAHLPEGTGPIRAARYGEHRLTAVAPNVTILRPGYFMENWANGIGMAKAEG
                     LLPSFIPPKMSMPMISTRDIGRVAAERLIAGGEGHAIVELAGPEEYSPEEVASELGRI
                     FGRGVSVQAAPLSAVVPTFTSFGFSTEAAGLFQEMYTAFSKDVVRYERPQSLVRGTKA
                     LAEALREMA"
     gene            complement(26433..26912)
                     /gene="smpB"
                     /locus_tag="PK589_12480"
     CDS             complement(26433..26912)
                     /gene="smpB"
                     /locus_tag="PK589_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNJ20469.1"
                     /translation="MTKTHDTDDQYKVVATNRKAYHDYFVEEKFEAGVILRGTEVKSL
                     REGRVNLQDSYASVDDGEVYLNHCHISPYSHGNLMNHDPLRKRKLLLHRKEINKLIGK
                     TQLKGLTLVPLRIYFSKRGHAKVELALAKGKKQYDRRESIKAREAGREVERAIKSRK"
     gene            26965..27462
                     /locus_tag="PK589_12485"
     CDS             26965..27462
                     /locus_tag="PK589_12485"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNJ20470.1"
                     /translation="MRGQSRLDSFGTILAGVAHRLGLESKLFEARLRRHWPEIVGEPI
                     ATHTRPDQIRFKKLHVYVHNSVWLQQLTFLKPVLLEKVNGMAGETLVTEIVLRIGEFS
                     AERKVSPPAATVEEPVPQPSAELLQETALHTQNIQDPALREQLAAVMAQALSGIPPDS
                     SRRAP"
     gene            complement(27407..28021)
                     /locus_tag="PK589_12490"
     CDS             complement(27407..28021)
                     /locus_tag="PK589_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20471.1"
                     /translation="MVMNQQQPPSAGTSAGRPPKRPLDTVVKLALGVLFSSFALIWGG
                     MFLSRPDRSIPPYSIGSQEGSIVAVHVPSWTSDTEIQTLIERFRKVGTETRNFGPMKI
                     RPTTADDPKGRYQRIAIYIFTDEGWTEPEMLHKYVTGEDPAVRDGFEGSARGYYKLDE
                     QEEEGRIGPVPKGKDSAATAAYSRQLFKGPVATSPAVSPTTPAP"
     gene            complement(28061..30520)
                     /gene="gyrA"
                     /locus_tag="PK589_12495"
     CDS             complement(28061..30520)
                     /gene="gyrA"
                     /locus_tag="PK589_12495"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNJ20472.1"
                     /translation="MPPDERLGQIAIEDEMRSSYLDYAMSVIVGRALPDVRDGLKPVH
                     RRILHGMNEMGLAANRPYRKSAKIVGEIMGNYHPHGDSAIYDTLVRMAQNFNMRYMLV
                     DGQGNYGSMDGDAAAAMRYTEARLTKLAEELLADIEKETVDFGPNYDESRVEPLVLPS
                     RVPNLLVNGAGGIAVGYATNIPTHNLGEVIEGLLLLLENPEVTIAQLMKKIPGPDFPT
                     AGFIYGTSGIKDAYETGRGLLTVRAKVAIETDERTDRERLIITEIPYQVNKSKLIEKI
                     ADLAQEDRVTGISDIRDESDREGVRVVIELKRNEIPLVVLNNLYKHSQLQTTFGVNML
                     ALVNNRPEVLNLKRILEAFVEHRREVVVRRTAYDLRKAEERAHILEGLKIALDNLDAV
                     IALIRRSQSPDVARAGLMQQFRLSEIQANAILEMRLQRLTQLERDKLVEEYREVLKTI
                     EYLRSVLGSEALVRKIIRDELTEIKEKYQDERRTKIVKEEAELTLEDLIAEEEVVVTI
                     SHAGYIKRNAVTLYRAQRRGGKGKIAMGIKEEDFVETLFTASTHDSLLFFTDAGKVYW
                     LKVHEIPEASRAAKGKALVNLLALSKDEKVTATLPVKEYRADRFVIMGTKQGVIKKTE
                     LSAYSNPRQGGIIALSLDAGDKLIGVDLTDGQREILLGTKQGITIRFKEEDVRAMGRT
                     AHGVRGITLEAGDEVIGMETITPDSTTAILTVTEGGYGKRTPVNEYRVQGRGGKGIIS
                     VKTTERNGAAVGFLQVRDEDEIMLMAAQGKVLRCKVDDIREIGRNTQGVRLLDMDGDE
                     DRVVAVVRLVEREEGVPEEGE"
     gene            complement(30699..>32372)
                     /locus_tag="PK589_12500"
     CDS             complement(30699..>32372)
                     /locus_tag="PK589_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNJ20473.1"
                     /translation="YNDGYAENLFSFANNINTKEGGTHLVGFKAALTRTINSYANAND
                     LLKKDTESLSGDDVREGLTAVVSVKVRNPQFEGQTKAKLGNSEVKGIVEAAVNDALGT
                     YFEENPPVARKIIGKAIDAARAREAARKAKDLIRRKSALDGGSLPGKLADCSEKDPAL
                     SELFIVEGDSAGGSAKQGRDRKFQAILPLKGKILNVEKARFDKMLSSDEIRTLILALG
                     TGIGRKKEDSDKADKDAFDIARTRYHKIVLMTDADVDGSHIRTLLLTFFFRQMPELLE
                     RGYIYIAQPPLFKVKKGKTERYLKDEPAMNEYLADLAVEGVEVALENGREFVTGRRLL
                     PILKKLIAFESLLHKVNKKHHEANMLRAFVDEPALTREVLKDPASLRTVVDHVKATLA
                     LVYPKAEPTIEILEDEEHQSSKLVCKVATGGIAYELQVTHELVGSADFRELQKQAPSS
                     IGLGKPPYKIKIKEGEVLKNGSAELVQAILEEGKQGLNIQRYKGLGEMNPGQLWETTM
                     DPEKRTLLRVNLEDMTGVDEIFTILMGDEVEPRRNFIQQHALEVRNLDV"
BASE COUNT         6377 a        10287 c         8828 g         6680 t
ORIGIN      
        1 ccggagctca gccgctcaga tgcggaaggc ttgcagggca attcgctgga gacgattcgc
       61 cagatggtcg cctccggact gggcattacc gtcatgccct gcagcgctgt gaccagcaga
      121 catttgaaca aacggctcac tgtgttggaa ttcgcgaaac cggtgcccga gcggcgcatt
      181 gccctggcgt ggcgcaaggg atttgccaga ccggccgtca tcgcggtgat tcatgaagcc
      241 gtggggatgt taaagatccc ggggctggaa tcgatctccg cacaggcctg acggatcgtg
      301 tgaaaggagc gtttatggag gacgcacatc ggatcagcat ccatgtgttg gatcaatcgc
      361 aaggacggcc ggcagaagga atcccggtga cgttggagat tcgagggctc accggtctct
      421 ggaagcagct cggccaagga cgaaccgatg cggacggccg tagctcggac ctctatcctg
      481 cgggcctccg catgcaaacc ggcacctatc ggctcacatt cgacgtggcc ggctattttc
      541 ggaaaagaaa cgtggcgacg ttcttcccgg aggtgccgat cgtgtttacc gtgcaggacg
      601 tggcgcagag ctaccatatc ccactgctac tcagtccctt cggctatagc acctatcgtg
      661 gcagttgagc gaacacgcga gtccgtctac tcgtgcgcac cggcagccag cagcagatct
      721 gagatcacgc ggtaaccctt ctgcctggca tgccgcagcg gtgtgacacc atcgtgatcg
      781 gcaagattcg gattcgctcc ggcagcgagg agcagacgaa cgatctcttg atgcgccgtg
      841 ccgccgtcac tcaataggat ggcctcaagc agggccgtcc agccgaggcg gttcacgtga
      901 ttcacatcga tcgtggtctt gagcaaggcc ttgaccacct cgacatggcc gcgctcacag
      961 gccgggatca acgccgtacc accgaaacga ttgtagaccg tgaagtccgg gcgcgcggcc
     1021 aatgtcattc gcacaatatc cagataacca ctggccccgg ccagcagcaa ggggctgttg
     1081 tgcatggcgt cctgcgcatt cacgtccgaa ccggcttcga tcagcaccct cgccacgtcg
     1141 acatggttgt gatacgtcgc ggccatgagt cccgtgcgac cctgcgcatc acgagcctga
     1201 accccggcgc catctcgcaa caaccgtttc actgattcaa cgtcattgcg ttccgccgct
     1261 gcgatcaaga gctggtcctt gtcgggagca ggggaggcgg tgacggccat gcctagcacg
     1321 accatgacga gaccggcaca catcgaagca agcatgacct tcatgatagc cgcgttccct
     1381 cccggcgacg gaccaatgcg cgtaaggcca tctgcatgtg cgcgtgaggc aaatgaatcc
     1441 gtcctccatg accggccagc acccattcga atggatagga cgtcaacgtc gcgatcgatc
     1501 ggcgcaatgc atcggcgtcc cagactaatt gctgagggga gcccaattca ttggtctccg
     1561 gatcccacca gagatgatca cccgtgaaca agaaccggtc ccgatatagg agacaaaggc
     1621 tgcccgccgt atggccgggg accgggatga tcagaaattc cggtcgatag aacatcgcat
     1681 cgagcccctc cacgatctct tccgcttccg gcatcacatg cgcatccgcc cggtgaatga
     1741 tgcgtctccc cccgaatcgc tgcgcgtacc tggcggcatc agccacatcg tcttcatggg
     1801 tcagaaagat gtgtgcgatt cctcccatcc gatcgaaggc ctcgaccaga ggtttgagat
     1861 atcgcggcga atcgatcagc cagttgccct ccggatgccg gatgaagaag ctgttcgcgc
     1921 cgaaggattt ttctgaattg aagccgcaat agtagacgtg atcttccacg aggagaggga
     1981 agtcggcctt cgctgcctgc atcacgccct tgtcgccctg ctcggttccg atcgatccgg
     2041 tggggcaggc gaggagcgct cggtaggcct ccctgatttc ctgcgcacta gccggttgat
     2101 gccgcacggc tgagtagtct ccgacttcct caaagctggc aggggcaagt tgacgacagg
     2161 catcgcaatt gatgcaggtg tcatcgacat aaaagttgcc ggggatattg gcgtctaatc
     2221 gcttatgcgg atcggccatt ggagactcgc ctggtcgaag gtttcgacag tgccgtcacg
     2281 gcttgcaggg cttttgcctg atcgaccgcg cgccacagat accaggcggc ggcagtccga
     2341 tacggccgcc accgttctcc atatcgtaac agttcttgag gggtcggcat cggccgcttg
     2401 taggcgatcc ggaagccgtt ccgcacgccg aagtcatcaa ccggcaggac atcgggacgg
     2461 cccagttgaa aaatcagcaa catctcgacg gtccaccggc cgatccctcg caccgccacc
     2521 agcctgtcga caatggcgtc gtcatcgagc cgtcgaatga cagcggcggt agggagggtg
     2581 ccatcgatcg ccttcgccgc caaatcctgc agcgcggcaa tcttcgcgcg cgagaatccc
     2641 gcttctcgca tgctctccgg ggccaaggcc aacacgtcgg ccggctgtgg aaaccggcgg
     2701 cccgggcaca acgcgatgaa tcgggtaaga atgcactccg ccgccttctc atgaagttgc
     2761 tggtaggcga tggctcggac cagcgcttca aatggagagc gccttggcgg aggaggcagc
     2821 gcataggggc cgaccgcgtc gatcacccgc ctcatcaccg gatcgatctt agacagatga
     2881 gcaatttccg ccgagggttc gctcatgggg ctattaccca atgatggcgt cggcttcgat
     2941 ttcgacgagc atctccggat cgatcaagcg cttcacctcc accatggtgg tcgcaggcct
     3001 gaccgaccca aacacctccc catgtgcgcg gccgacttcc tgccattgat cgatattggc
     3061 catataaatg cgcgtccgca cgacatcaga aatcgatgcg ccggcctgca cgagcgccgc
     3121 ttcaatcgtc ttcaaggcct gaatggtctg cgcgtaggga tcattcttcc ccacgagtcc
     3181 tgctcccgtc atcgccgtgg tgccggagac ctgcacggat tgaccgaccc gcactgcccg
     3241 cgaatagccg attttgcctt cccaaggtcc cccagtcgaa acgttctgcc ttcccatgat
     3301 tcctcctttg atcgtctgcg aacactccac tttacacggc acgcaggatg cgcaaaaagg
     3361 ctttccagca aggccccagt gaatggagag ccgaagcgta ccccttacgg tacgttgagg
     3421 ttcgccatga agcgagaacg ccgctggagg ctgtgttccc catcctgcta cacgttacat
     3481 caccaggccg ccgcctgcat gaatcacctg gccggtcaac catcgggcct gctcactcac
     3541 tagaaacgcc accacatcgg cgatatcaga aggatgtccg agccgctgcg tgggcgacat
     3601 ctgcgctccc atctggcgat acggctccgt caagactccg gtgtccgtaa aaccgggaag
     3661 cacggtattg accgtgatcc cccgggaggc caactcctgc gcgagccctt tgccgtactg
     3721 ttccagggct gctttgctcc ccaggtgcgc ggtggcgccc ggaaacttca gatgggtgag
     3781 cgccgacgaa atattcacga tgcgtccgcc gtcgctgaga acccgcgccg cggcctgcat
     3841 ggcaaagtat ggtcccttcg cattcaaagc gagaatcgca tcgaactcct cttcggtcgt
     3901 ctccagtagg ggacgaggaa gaaaccggcc cgcgttgttc acgagaatat ccaggcggcc
     3961 taactccttc gctgtctccc gcaccaatcg ctgggcatcg gccacccggc tcatgtcggc
     4021 ctgccgcacc agggcgctgc ctccactgga ctgaatccct tcagccacct ggttggcctt
     4081 ctcggcatgc acatggtagt ggaccacaac ggtcgcgcca tccctggcca atcgttcggc
     4141 aatcgcccgg ccaatgccgc tggaagaccc cgtgacaatg gcgactttgc ccgtcaaagg
     4201 cctcattccg gcacctccgc cttaaccatg cgttccattg atgatcccgt tcgcgcaatc
     4261 gttgtggann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc tccgccttaa
     4381 ccatgcgttc cattgatgat cccgttcgcg caatcgttgt ggattagaaa gaccgttgca
     4441 ttgagcctgg acggtctgcg gcagacagcc cggcgcctgt acactgtatc aattcagctg
     4501 agccgagatc caacggcaat tcttacggtg cgcaagatag gaagcaccag cgtgagaata
     4561 ctatgcgtag aggacgggcg aggatcggac cgttgtgtct cgggtccgtt caattgatag
     4621 gaaggagagg cgcctcctat ggtttcagcc atccaggctg atcattccag gtaccgtgag
     4681 agacgaattc cgtgctgagt ttccgttcgc gtggtttgaa ttctcgttcg cgtagatagt
     4741 ccggcagctc ggccgcatca ccaggatatc cgacggcgat catcgccacc ggctcatgcc
     4801 ccgcaggcag accgaggtcc gtacgagcct tctccatgtc gaagcctgcc atctggtgcg
     4861 cgatcagccc catcgccgtg gcctgtaagc acaagctcag cgccgccatt cccgtatcat
     4921 gcaaagcgtg ccggtttggc gtgccttcat cctcgaaatt cagccgcgcc acggagagca
     4981 tcagcacggg cgcccggaac gcccattttt tgttgccttc cttgagacag gcaaacagtc
     5041 ggtcatacgc cgccgggtcg gctttcgtgc cgacgatgaa atgccatggc tgttcgttgt
     5101 tcgaagaggg agcccatctg gccgcttcaa acaggcttct caacttctcc ggttcgacca
     5161 tccgttccga ataggcccgc gggctccagc gttgccgcag cacatcatga atgggatatt
     5221 gcgtctcagc gggcttttcc atgaacgatc cttccgtgaa ttattctgtt gtcagggtgg
     5281 gtgagcccaa tgtgcgcacc acgcgtgccg gattgccgac cgcgacacaa tcggcgggga
     5341 ccgagtgcac gaccacgctg cccgcaccca ccacgctgcg atcaccaatc gtcacaccgg
     5401 ggagaatcac cgctccgccg ccgatccaga cgtcactgcc gatccggatc ggtcgcgcag
     5461 actccaggcc cgaccggcgt atctccgcct cgagcggatg ctgcgccgtg tagagctgca
     5521 cggcggggcc cacttgcaca tcattcccga tatcgatagg ggcacagtcg agaaaaatgc
     5581 agtgatagtt gatgaacacg ttccggccca ggcggatgtt gtagccgtag tcgcacgtga
     5641 actgcggcat gatgacggtg ccgtctccga cggaacccat caaatcacga agcagtgaca
     5701 gcaggagatc aggctcacca tccgtgatgg cattatagcg agcgagcatc atctgcgcgc
     5761 ggcgacgctc catggtgagc tcagcagagg atgcgcggta cagctccccc gccaacatgt
     5821 tgcgtttgtc gcgttgttca gtcatcgcgt tcagcacgtc gcttacatga gtgggtcagg
     5881 ccgaaccccg cattcatcca ggtgacgcag gagatcggcg gggtcatggt aggtacggta
     5941 cgcacccgcc cgttccaatt cctctcgacc atagccgccc gacagcaggc caactccaag
     6001 ggcagaggca cggcgggctg ccaggaggtc ccacacactg tctccgacca cgatacactt
     6061 cgcaatggga accttgagct gttcggcagc agcgagaaac aaatcaggat ccggtttggc
     6121 ccgtttcacc tgatcacgcg tcaccaccgg cgtgccttca ggcaacttca gcaatttcaa
     6181 ggtatgttgc gcgctctgga gccgtccgct ggtcgcgatg gcatagggca cacggcccgc
     6241 cgtcagcgca tccagcagag cctgcgcgcc gggcaagatg cggagtgatg gcgcctgcct
     6301 cacgaacgcc tccgcgtgga ctcgctgaat gcgctccgcc tcctccttcg aaacgggacg
     6361 accggtttcg cgcaagaggg catgcaacat gagaccgccg ctcatgccga tttggcgatg
     6421 gatcctccag accggcaatt cgatacctgc ggcttgagtg gcttcacgcc aggccagcac
     6481 atgttgatag acactgtcga ccaacgtacc gtccaggtcg aagaggaacg cgagcccgtc
     6541 atgctcatgc accatggcca tatctgtcag ctctccaact tggcgtccat ggtgatggtg
     6601 gtattcagta agcgggaaat ggggcagccg gttttggccg ctgtggtcgc cttctcccat
     6661 gccgcattgt cagccttggg cactttcccc tttacgtcca ggtgaatgcc ggtcacggtc
     6721 catcctgcct caagcttctc catggtgact gtggcggtcg tcgccagctg gtcggccacg
     6781 agattcgccg cgcccaattg accggagagt gccatggtaa aacaaccggc atgggcggca
     6841 gcgatcaact cctccgggtt cgtccctttg ccgttctcga acctggtact gaatgaatat
     6901 tgcgtcccgg ccaacacacc gctctcggtc gagaccgtcc ctttgccggt tttgagatct
     6961 ccctgccact ttgccgacgc attgcgtttc atgaccacct ccgttgttaa gaattcatcg
     7021 tcaggacggc gcgaaagcgt gccttgccgc tcatcatctg ctgataggct tccggtgcgc
     7081 gcgccagtgg ataccgttcg atcatgggcc gcacgccggt catcgcgctg aatcgcagcg
     7141 tgtcttcaga atccttcgcc gtgccggacg gccagccctg cacagaggct cgtttaccga
     7201 tcagcgtgat ggcattcacc attaccggtt cgcctggcgc ggccacgatg agcaaggttc
     7261 cattcgcacc cagtccgtcg accatcgatg cgatcgcttt gctgtccggc gcggtcgcca
     7321 gaacgacccg ggcgccgccc agtcgttgaa gctcctcggc tggattcacc accgcggcat
     7381 cgagatagac cgccgcaccg agctgacggg ccagcggctc cttgtcttta ccgcggccga
     7441 ccgccactgt cttgagtccc agcttcgagg catactgaat gccgagatgt cccagcccgc
     7501 cgatgccttg cacggcgacc aggtcacccg cccgagcacc actgtggcgc aaactattaa
     7561 aggtggtaat gcctgcgcag agcagtggag cggcctcttc aaacgagagt tcatccggca
     7621 gggcagccat cgcttgctcc ggcaccacca catattccgc ccaccctccg tcgtagctga
     7681 tcccgcagac ctgccccgac cggcagagaa taaaatctcc ccggcgacag gcgtcgcact
     7741 ggccacaatg gcccccgtgc cagcccacac caacacgttg accaggtttc cacgctgtga
     7801 cagcggatcc cacctcatcg actaatccgg caatttcatg ccccgggata cgggggaatt
     7861 gcagccccgg ccaatgccct tctttcacaa acatgtcact gtggcagata ccacaggcct
     7921 ggaccttcac ccgcacctga tcaggtttcg gtctgggaat atctcgttcc accagctccc
     7981 atgcagctcc cggagcagtg gcttgtacag ccttcatgcg cgccatcatg atgccctttc
     8041 tgaatacacg tatgtggcac cggcgtggcg caacctgtcc ttaacggaaa gactgcccca
     8101 cgccctctat cttctgcgac cacgtcctac ccgggagcgt gaatcgatga acggccgctt
     8161 cacttctcga cgtcgaagcc gtcgtctttc caccatcgcc accctccctc caattccatg
     8221 atcggatagc cctgccccgc aaactgcacc gcagcggtgg cggcaagatg gcagacttgc
     8281 gaataacagt agaggacgtt gaccttgtcc ttgcgtagac ctttgaggcc cgcccactgg
     8341 tccttgggca aactgaccgc gccgggaata tgcccttcgg cgtaatcttc ggccgcgcgc
     8401 acatcaacca cattcacttc tttattcttc atcatccgct ccagttcgac cggaccggtg
     8461 gtaaatgcca ttttcgcctc gaagaactcc ttggctttgg ccgagtcgtg cgccatcact
     8521 ggtgtcgcca tggtcatcct ccctctgaat cgtgacgtgt gtatgctgtt cgcgtcggac
     8581 tggcctccca ggctcaagcg ggacgccagc ctcacgggtt ggcttctacc ttgtaacgtt
     8641 agagagtctg gtgcctctat gcaagcgctg atcgctcgcc ttttggatgg ctcattggaa
     8701 ttgtcgtcga aaccgccaca tcccggaacc gaacagcgcc ccgcccaaca acagcatggc
     8761 accgaccggc ttccacaaca gatccaagcc ggcccccttc aacatcacgc cgtaggtgat
     8821 gtcgatgtag tagcgaagag gggagagggt catgatcgcc tgcacccatc gaggcatcga
     8881 ttcaaatggc gaggtaatgc ctgacaacaa cagcatcggg cccaccagga agagcgccat
     8941 catggccact tgcgcctgat tcttcgccag agtcgccgcc atcagcccga atccggccgt
     9001 cgtgaagaca tgcaaggcgg tcagcagaaa aaagagtagt gccgatccct tcatcggcac
     9061 gtgaaacacg ggatgcaaca cgccgtacag ggccagtgcg gtcgcgagga gaatcacccc
     9121 ggccatggcg agcactttcg aaaacatgat ctgaaacggg gacaatgggg acaccaataa
     9181 ctgctcgacc gtgcctcgct ctttttcacg caccaacgcg gcggccggca acaacaccgc
     9241 gaagagcgtg atcatccgca acagatgcga aatggactgg aaccatcgtt cgtcctgggt
     9301 cggattgaac cagacacgat gcgcactgga gacaagcggc atgctcatct tcgttccgga
     9361 cagacctgca gaggccagag ccgtctcggt gccgaacggg acagcaatgc gcacggtata
     9421 ggcggcggcg gaaagtccct gtggcgcatt ggtagtatcg accagcagtt gaaccgccgt
     9481 tcgctccccg ctcacgaggg cttcgtggaa tctcggagga acatccaaca ccaccatcgc
     9541 ctggccctga tccagttgct gaagcccttg gcgcgggttg ttgatcgtgc ccaccacagc
     9601 aaaatagggg ggctgaaacc gatgaatcag ctctcgtgaa gagggacttt gatcctcatc
     9661 atgcaccagc agctccgcat tggtgagctg catcgtaatc ccgactccgc tgaccagcac
     9721 tgacagagaa aatgaataga tcaggaataa taacaacggt acatcgcgcg acagttgcag
     9781 gagttccttc cacgtcagcg ccgcaagtcg ttgtgtccac ctggctgccc gctgcccggc
     9841 cgtcatgcca tacccctcat gctttgggtc gcttggtgaa gagatgatac gtaagcccac
     9901 ccaacccgcc ggcaaagagg gccagcgcca gtagatcctg ccacaggacc tctacgccga
     9961 cgcctttcaa aaaactgcct cgcacaatgt tggtgaagta catggccggg aaaagatggg
    10021 cctgcacctt cgcccctcgt gttaaagagg caacgggaac cagaaggccg gaaaacaaaa
    10081 tggtcgggat catggccaca accatcgtga tgatcaaggc tgccatttgc gtctgcacca
    10141 gcagcgaaat cagcaggccg attcccgtgg tacagcagac gaacagcacc gacgcgaaaa
    10201 agaagagaag gaagtgtcct ttaaacggca cctgaaacag gcccacagcc atcagccaca
    10261 acacgcagac attcacgagg gaaatgagaa tgtaggggag cagcttcccg gtgagaaatt
    10321 cggctcgact gaccgtggag ctgtagatgt tatagatcga gccggtttct ttttcccgca
    10381 caaccccgag ggccgtcagc agaggggagg cgagcatcaa cgtgaacatg accagcgccg
    10441 gtaccatgga ccaggtactc cggacctctt cattgtacag ataccgaacc tctacactca
    10501 gcggccgcac gaggacgccg gcttgctccc gggtgaggcc gcgcgagcgc cgcaggaaat
    10561 cgattaatcg atcctccgta aacgcctgat tgatcgcgat gacgtagccc ttggcgatat
    10621 ccgtgtgcag tggaaaggtc ccatccagca atgtctgcac ggcgaccgct tctccggcga
    10681 ccaattgttc ctgaaaccgt tcggggacga tgatggctgc ccgaatcttc gtttcggtca
    10741 acagccgatc caggctgtgc tcgtcgtggg cataccctcg aaatgaaaaa taccgcgact
    10801 gaatgaaccg ctggagatac tcccggctga actcactctg gtcccgatcc agcacggcaa
    10861 agggaatgtc ttcgacgtcc agattgagcc cgtacccgaa caccacgagc cagagagccg
    10921 gcaggagaaa ggccaacagg aggaacagcc ggtccctggt ggtctccttc cactccttga
    10981 gcgccacagc gctgatgcgt tgcaggttca ggacgccccc ttcttctgcg ccgcctgttc
    11041 gagcgccatc acacgtgaaa caaacacgtc ctccaagctg agcatccgtg gacgcacggc
    11101 cgcaaccgtc gtgtcagcgc ggcccaacac ctcccgcaga cgcgcctcgt cacgagaagg
    11161 atcgcgcgag agcacatgga tcttggtgcc gaagagcgcg gcaccggtga acccggcggc
    11221 cgtcacctgc gccagtgccg cacccggctt gtccacggca atctccagca gttgccccac
    11281 ttcgtgttcc acctgggttt tgagatccgc cggtgtccct tcggccacga tatggccggc
    11341 atacatgagc gcgagccggt cgcaatgttc tgcttcgctc agataatggg tggtgatcag
    11401 gatcgcgacg ccttcctcgc gggccaaccg cgtcagcaaa tcccagaatc gacgacggcc
    11461 gatgggatcc acgccggacg tcggttcgtc caggaacaag acccgggggc tgtggacgag
    11521 tgcgcacccc aacgcgagac gctgccgcac ccccatgggc aggcgacctg tccggtcatg
    11581 ctcatagccg ctgaggcccg ccatttccac gatccacgcc atccgttgct gcgcctgccg
    11641 tcgagccaac ccgtagatgt ccgcaaacag ccggatattc tcgatcacgg tcagatccag
    11701 atacagggag aaggcctggg acatgtagcc gatacgttcc ttgatggccc cgctggccgt
    11761 ccgcatgtcg gcgccggcca cgcggccctc tccgcccgat ggcggcacaa tccccgtcaa
    11821 catcttgatc accgtggttt tgcctgcgcc gttggctccc aggagtccga agatttcgcc
    11881 ttgttgcatg tcaaaactga ctcgatccac cgctcgaaat gaaccgaagt cacgaatcaa
    11941 ctctttagcc tcgacggctg gtccgtcgaa ctgcggcctg ttcgccgggg gggctgactg
    12001 acttgatcgc tccgatgctc tcgtctgacc gtcctgttct ttcagcagga gcgccacgac
    12061 gacatcttcc agttccggtt catccgtgcg gagatgtgcc accggtaaag gatccagcac
    12121 ctgccggatt cctgcaacgg cggcctccgc atcctgctcc ggactgaaca cgtggagcga
    12181 cgggcccaac gactccacct gggcgtagcg gtgtttcaaa cgttcaaccg cttcgagttg
    12241 cggcgttgat tcgaaggtaa cgaccacgcc gggcactaac gattgcacgt ctgcaggagt
    12301 accggaagcc agcacgcgtc catgggagag aaacgagagt cgatgaaagc ggaccgcttc
    12361 gtccatgtag gcggtcgaga cgagcgcggt catgcccttc acggcctgcc attccgcgag
    12421 aatggcccag aaatcgcggc gcgacaccgg gtcgacaccc gtcgtcggct cgtcgagaat
    12481 ggcgagctcc ggctcatgaa tcagcgtgca gatcaggccg agcttctgct tcatgccgcc
    12541 cgaaagttgt ttcatcaacc ggctgcgaaa gcgatctaag cgtgtgatgc tcagcaaccg
    12601 tgccttgcgc gcctgaaggt ccgtctcggg aaccagccgc aacttggcaa agaaatcgat
    12661 gttctcctcg acagaaagtt cggggtagag attgagtccc aatccctggg gaagaaatcc
    12721 gatccgccgc ttcacctgtt ccgctgcccg ttcggaatcc aagagggtcc caaacacgtc
    12781 gacggagccc tcctcgtacc ccaacacccc cgcgatggcc ttcatcacgc tgctcttgcc
    12841 cgcaccatcg ggaccgatga ggccatagat ctcgccacga tgcaccgtga agtccaagcc
    12901 attgaccgca cgatgctgct tgtatcgctt gacgaagccg gacacccgga cgacctcatc
    12961 cgctgaggac gcaggcgggc ccatcatcgg ggcttcgacc aggcgacgtc gtctttccag
    13021 cggatcaccg cgtccgccgg cagacccggc gtcagccgat gatctggatt cgtcgtgagg
    13081 tagagccgga cggcatagat caacttgacc cgttcgtccg gagtttggac ttctttgggc
    13141 gtgaactccg ccttggaggc gatgtatcgg accgtggcgt cgaagggttg gtcggggaac
    13201 gcgtccgtgt ggatgcgggc cggcaaatcg agacggacct tgccgatctg gagttccggg
    13261 acatagacct gcaggtacag gcggtcgagg tcgactaatt cgaggagtgg tgccccggta
    13321 gccacgactt cccccacgtc gaccatgcgc gtggtcaccg tcccgctcgt cggcgccaca
    13381 atcgtcagat catcgagcac gctgcgcgcc tccgccagcg tggcgtccgc ctggtcgcgc
    13441 tgccgttcga gcgcggcgac ttccgcttct ttcgaccgga ttcgctctcg ccccaattcc
    13501 gcctgggcca gttccttgcc ggcttgatcg tgagcggcct gcgcgacagc aatctcggtc
    13561 acggcaattt tccaccgcgc atcagcctga tccacttgct gctccgacac ggcctgctgg
    13621 ggaagcaggg cacggatgcg ctcggcgtcg cggcgggctt catattcgac ggcgcgggcc
    13681 ttccggatga cggcgcgcgc actctcaact ttcgccgcgg ccgcctcgat actcagaggg
    13741 acttccaggt tcaggaccgc gagggccgtg tgagccgcct ccacctgtgc catcaggcta
    13801 tcgaccagcc gcgcggcttg atcgaccttc gctttggtct gcgcatcatc gagccgaatc
    13861 aggatgctgc ctttgagcac ctccgcgccc tcacgtgccg ccagctcgac cacacgtccc
    13921 gggaacttgc tggccacagt gacatggtcg ccttcaatgc ggccattggc ctgaatcagt
    13981 ccatccggca accggttgtg ccataccagt cgatcgagga gtacatagcc ggctatcacg
    14041 acgacggcga ggacggcggc ggccgtcatg gaggtgcgga tagtcatgca atcagtctgt
    14101 cctcgaggcg tggaggcaat cactcctgca aggccccatt gccctcgcgt cgacactgta
    14161 gtcgataggg gacctttttg ccagagacgg gcgcctccgg cagctcggcc caccatcgac
    14221 ctgctggctc agtggtctcc gtaccggtcc atccacttgc ggatatgttc aacggcctct
    14281 gctttctgtt ccgggcgaag cgtcgcatgc cactccgtta gcttcggcaa cacccgctgc
    14341 atcacgcgct tctgttcggc ttggtgccga tccatcaatt ggatcagttg ggcctgatcc
    14401 agccggtcgc tctgcacctg ggcaatcgcc gcctccatga tcgccttgtg ctctgcctgc
    14461 gactcggcac gagcggccac catttcgtct ctgacggcca tcaacttcgc cttctgctga
    14521 tcgtcgaggt ctaagtgttt ggcgatcttg tttgtcatcc aatccgcccg ctccgccggc
    14581 gtgtggtgac ggtgacaacc ggcgcctacc agcgccccgg ccagaaccag tactccgatc
    14641 gcaacccgaa ccgtctgctg catcttcatg ctgccctcct tcgtgaatga cctggtgctt
    14701 gagcgatggc cctcatgcac atttaatcag tattcgaagg cgacgtggta gagtgccggc
    14761 gtcatatcca ctcccaatgc gccgagtttc tccttcatgg gcgtgacgct cttctcgtag
    14821 gccttccaat acagggtctc gttttcccag atcgccacgt tgatatagtt gaaccggctg
    14881 tccggcttga gactcttgtg aaacgtgccg ctgatgaatc ctgcttgcgt tgtgatgtgc
    14941 gctttgacgt cctgccacca ctgtacaaat tctgtttctg tggcggccgg gacggagaat
    15001 acgttgatca atgtcaccgg catgatgttc ctccttgata ggattcactc gaaactcgaa
    15061 cgggccttac ggcgatgtga tccgatgtcc accttcatgt gaagaggtct ggcggagtag
    15121 ggcacccgcc aggtctcttc aggttattgc gccttccgca ggtcgatcac ggttccttct
    15181 tcgttcaact cgaccacgat atgcgaccct tccgcaatcg gcttggtctt cacttccatc
    15241 cgctccagcg gaaattcttt tacgccttcc ggggtcgcca gcttgatcac attcttcgtc
    15301 gtgcccgcat agatcaattt cccgaagatc agccggtgac tgcctgcctt gtgcttgtct
    15361 tttccgtggt gatcgatgac catgccttct tcattgatcc agagcgacac gtcatcgccg
    15421 acggccgcat cggcaggggc ggtgttcgcg ctgattgtat attgtccggc cggcgtcttc
    15481 acatagacca agccggattg gatcttggtc accgtcccat ttacctgaat gacgtaaccc
    15541 ggcttcgtgc gcggatgctc gtcgaagctc atctcaaccg taaagcgatt gatgtcgatc
    15601 atctttcccg cttcgttcaa ttcgaccgtc accggcgccc cttcttccaa ggccgataat
    15661 ttggaccgtc cggtttgcac atcaatggct ttctctcctt ccggcgtcca caccttgatc
    15721 tccctcctgt ccggcgcagt ataggccagg ttgcccgtca cgaatcgatg gctatgcgcc
    15781 ttgtctccct tccgatggac gtcgatcacg gcattgtttt cattgacctg catctctacg
    15841 tcttccccca cctcaagatt tttgggcgtg agcgtggacg cgattttcat cgtcccccat
    15901 ggagtcttca cggtggtgat gcctgattgc accttcgaca ccacaccact gaccttcata
    15961 tgcgtcgcgc tcttcccgcc gccttcggcg gtgaatccgg aagacgctcc cagcgccaca
    16021 ctcagtacca gcgaaccgag aacaatcatt gtgcgtgtca tgatacgact cctttcattc
    16081 ctgactgagg accaccttgt ctaacctctg cacgatggcc ctcgtcaggc aacccgaagc
    16141 ctctatggtg acccccgcgc gctgcatctg cgacagcaac gtggtgcccg cctcatcgat
    16201 gaacgtcact tcccggagat cgatatggca tggggccggc tgatcgatct cctgcttcac
    16261 ccaacaggtc cgcaactcgt cgacccaggg accagccaga cggccggaca gcacgaaggt
    16321 ggttcccgtg cttgtgttcg acagcgtaat cttcaacatg gtcgtggacc tctctgtccc
    16381 tcgaggacgg ccgggaagca acccggcccc gccatggatg atctcagaac atgcgcaatg
    16441 cgaagctcgc ctcccgcggc ttcctcaccg gaagggccgc tcgctcgccg aaccagcggg
    16501 caatcaggcc atcaagcgac cacttgcctc caccgatgat cagcaacgcc aggctcatac
    16561 cgatcacgag caggtgatat tcgaaccctt caccggtctg ctgtccgaac cagttcatga
    16621 agaagccctg gggcaagtgc accgtcacaa tcgcccccaa catgatcacc acaaagctcg
    16681 ccgccgtgaa ccgcgtgagc aggccagcaa tcagcccgag actaccgatg gactctccga
    16741 tgatgacgag gaatgcgacc aaccaaggga agcccatttt ttgagtaaaa aatcccatgg
    16801 tgccctcaaa cccgaaccct ccgtaccatc ccaatagttt ctgggcgcca tgggcgaaga
    16861 tcacacctcc cagcaccacc cgcaaaatca aaccggccca ggaatcatct gttttgaaaa
    16921 agctgtacat gtcacacctc cttgatatga attaccgccg gtgactccgc acccattgca
    16981 gaaccgccgt ctccgtcctg tctttcagca acagccccgc cacgaagagg ggcagggtca
    17041 ggagcattcc gtaccagccg acccagagcg tgatgtcttt catgaattcc ccccgttccc
    17101 ttgtgcgttg ctatgcaggg gtaatggcaa ggcctgttcc aggcgctgga cgatcacgcc
    17161 acatgggcta actgcttgaa atcctgatct gattgtccgt gggtcgaaga aaacgatggg
    17221 ctgacaaaag atgcgtgacg aagggcgtgc gacgagacgt cgtcaggtga cgaaacggct
    17281 cgtcactctc gtgggcgaga gagtccgagc tttttgattt tcgatgtgag ggtagtgcgt
    17341 ttgagaccta atcgagccgc cgcaccatcc gggccgccga tcacccatct ggtgtcgcgc
    17401 agggttcgga gaatttggtc gcgttcggca tcctgcaggg tggctcctgg tgccggggcc
    17461 tgccgcgcct cgatcttcag ttcctgaagc ggcacctgaa gctgcgtccc tggagtcagg
    17521 atcacagctc gctcgacgag attctccaat tcccgaatgt tgccgggcca gtgatatcgg
    17581 gacagggcct ccagggtcgg agcgggaatg gattcaatgg tcttcttcat gcgcgcagca
    17641 tagtgctgtg cgaaataccg cacgagcatc ggaatgtcct cccgccgatc ccgtagcgcc
    17701 ggaaggttga gcggaaacac gttcagccgg taatacaaat cgccgcgaaa ccggccttcc
    17761 tcgaccaatt tggccaggtc tcgattcgtg gcagctacca gacggatatc cacgcgaatc
    17821 gtgcgcgtac tcccgagacg ctcgaattcc tgctcctgca gcacccggag caatttggat
    17881 tgcagctcca gaggaatttc tcccacttcg tccagaaaga tcgtgccgcc atgggccaac
    17941 tcgaaccggc cgactttttg ggcgatggcg cccgtgaatg cgccccgttc gtggccgaac
    18001 aattcactct ccaacaatcc ggtcggaatg gcggcgcagt tgagtttaac gaacgtgcgc
    18061 gcatgccggc cactcagacg atggatcgcc ctggcaatga gttctttccc ggtaccggtt
    18121 tcgcccagaa tcagcaccgt cgcgtcggtg ggcgccacga tctcgacttg ccccaaaatc
    18181 tgcttcaagg gccggctgtc gccgatgatg tcgtcgaagc cgtgctcgac acggatttcc
    18241 tcttccagat acaacttttc ctcggtcaat cgatctttaa gcgtgtgaat ctcctgatac
    18301 gccacagcat tgtcgacggc caaggcgatc tgccccgcca cctggctcaa gaattcgaga
    18361 tcctgttcgc caaaggcttg ttcgtgatga ctcgccagat tcaggcagcc gatcacgcga
    18421 tcgcgcgaca gcagcggcag tgagcacatg gagcggaacc ctttcctcac caggagttga
    18481 ggcacggcat gggaaaacgc cttcaggtca gccagggtgt tggcgaccgc ggggcgcctc
    18541 cgctcaaacg ccagaccggc cagagacccg gcgacatccg atagtgcccc ttcggtcaac
    18601 gcgccttcgt tgtcgggaaa atcgagcgca tgcagacgga cgcgctgcgt ctggccgtcg
    18661 cagaggatca aactggcgta ttcctgaggg accagctcgc gcagacagag gctcaccgcc
    18721 ttgaacacct ggcgcagatc caaggtcgac gccatcgaat tcgtcatgcg cagcaacaga
    18781 ctgaagcgat cccgttgccg ttccacgtcc cgttgtgatg ccgctgccgc ctcgcggttc
    18841 agcacgtttt ctactgccac cgccacttgg cgtccgactt gctgcatcaa ttccacgtcc
    18901 gacgtgctgt aggcgctctg ctcaagactg gaaaactcca aagatcccag tcgctgcgtc
    18961 ggggacgtca gcggcaccag acaacatgaa cgcactccgt cctcgcacat caattcgatg
    19021 accttcggcc agcgtcgttc tgtgggcaga tcgttcagca agagtggctg ctgggtttcc
    19081 cacaccagac ccgccggcgt gtcttgggga tcgctttcgt gccctccgat gatctccgca
    19141 ggcacattcg cccgcagcac ctgcagacgc atcaccccac gctgagaatc atagagagac
    19201 agtccgacga aattgacggg tacgaccaag ggcagcagcc gcgcaagatc ctgcactagc
    19261 gcagacaggt cggagtgact ggcaatggct tccgcgacgc gcaatagcgc ccgataccgc
    19321 tcgatgccgg aggaatctct gtcgctcacc gaggtcctgt catgtgtcat cacggaatga
    19381 gcaacgtcgt tcgtgacgct gcagcctgcg ttagtacccc gcgaactttt cgattcgaat
    19441 atcggtgtcc tcgacgtgag cgtctttcag aatggtccgc aatgcggtca ccatgccggg
    19501 cggaccgacc acatagtaca tcggcttgtc gatctccgcg agatacttgg agagcaagcc
    19561 cgcattcagg taccccgttt cgcctcgcca ggcatggagg gatttctcgg gttcggtcat
    19621 ggtacccaca aaaatatagt gacgattctt gtcctgtaaa gactgcagct catccaaaaa
    19681 cgccgcatcc tccggtcgcc ggttggagta gaacagcacc agacgctgcg gtgattgctg
    19741 ctgggcggca tgcaccacca tgctgcggaa aggggtaatc ccgatgccgc cagctaccaa
    19801 gacagcgggg cgagtctggt cactgtgcaa cacgagtttg ccgaacggcc cttccatccg
    19861 gacggttgag ccgatcggca tacgttgcaa ctcgcgcttg aaggcggtgt cgcggagacg
    19921 cgtcgcgacc atgatggtgt cctcatgcgg ggcgctggcg atggaaaagg ctctgctgtt
    19981 gccggccgca tcggtttccg atggctggag taacgtcagg tccacgaact gtcctggagt
    20041 gaacacaaac tgagacggct tggcgaaatg gaacgccatc gttccctccg cgatcaaccg
    20101 tcgttcgatc agtttcaccg gaaagccgct gtcatccatt gcgtcactca tccctgacct
    20161 cgcatgacat tacaggcgat acgtccccac cctgttacag cgacttgtgc gagccatcac
    20221 aaaacggagg attcttcgta tgcttgcact gacacaaggc cagggttttc ttttcgaccg
    20281 tcttaaactc catgggcgtg aagctggttc ccttgtgaga tccatcgcaa aatggttgct
    20341 tcttcgagcg gccgcagcgg caccaataat atgtccctgc ttccaattcc aatacggccg
    20401 gctgcttcgc tgcaatgact ggttcttcca tgtgcatctc ctcctggttg actgactgtt
    20461 ttcatgtcca catacgccct tgccctgcct agtcccgctc gatgcatgtc atgaatcgca
    20521 catcgcttgc ccctggaaca ggtgatgagc tcgtcccgcc acgcggctgc atcacagaaa
    20581 agcacattgc cgcggaagaa tgtgcccatc tccagccggc tgttgcgaca gaaggggaca
    20641 gcgtgaatct ggccttcagc gctaaagcac cagcggtttg atcggcgaga cgaccgcgtc
    20701 aggattagtt ttccagatcg cctcgtccgc atcgacatac agctccacct cgttgccatc
    20761 cggatcccga aggtacagac tctgactgac ggtgtggtca ctcatgccgt cgatcgcgat
    20821 gccggcctgc tccagctcgc gtttagccgt cctcagttcg tccaggctgt ccccgacttt
    20881 gatgccgata tggtagagcc ctcgtctccg tcccggcggc ggtcccggtg catcgccgac
    20941 ctggatcaac agcaattcat ggtgcgtgcg gccggatgtc agcgcggctg cagcgccatt
    21001 gaagatccgc ccgacttcgt gaaaccctaa caaatcgcgg tagaacgcca gcgcacgctg
    21061 cagatctttc acgtaaaaca cgacatgccc taaatagtgc gctttcatcg tcgtgactcc
    21121 ttcctcggcc tgtcccgtta tcgaagcagc gcccgaaccg ccgcgtgagc cagcaccaca
    21181 tcaggctccc gttgcaaggc atcataatag ttccggccga aaccattgcc ctcttcggaa
    21241 ggggtcaaca gggtccgcac cgacccgatc accgcggtga tctccccttg atccgacgcc
    21301 tctttttctt cggacaactc atccaatcgc gccattagtt gggagagcgg acgcaaccag
    21361 gcaaaccagg catccccgag gactaactga aaaaacgcgc cattggtagg aatgcgccca
    21421 tgaacccgct cgtaggagac tcgttcggca tccaacaacg ccttatgaag gcgcaacagg
    21481 gcgcgagaca cgttatccag atgcgccgtg gtatgatcct gacccgacat acgtccgcct
    21541 ccttacgatt ggctctccat tatcccgtcg gttcactgtc gagttgatgc gccttgcggc
    21601 cgcctcgggc gagcggagcg gagatcaggc gcgcctcaag catgagccga gccaggtccg
    21661 ctgcgcgggt gacgagcgct tccgcccctt cgcgcaattc gcgactgcct tgcacctccc
    21721 gattgttccg ttccatgtct tccgcactcc acccacgcga gtggtcgata aagggaaatt
    21781 gcggaaactg acaaccgact tcggcgaaaa aggtcatcaa ttgtccggcc acgccctgca
    21841 cattatcctg tccgcccatg atgatgaagg cggccacttt gtccttgagc agatgccggt
    21901 tcgcgatcgt ctcttgattc tgaatacagt tcagccgttc gaccattttg taatacaggc
    21961 tgctggcccc gccccatctg atcggggtcg acacgagaat cacgtctgcc caatggacaa
    22021 taccttcata gactcgatcc atttgatctg ccgcatccat ttgggtgatc gaacagggcc
    22081 aggtacaggc ttcggccgct ttcgaataaa acccttcaca aggccggaac gacaggtccc
    22141 gcagccggat gagctgcgtg tcgagctgca agtgctcgcg cgcatgctcc agggccgctt
    22201 ccagcagcgc atcggacgcg ctgtatcgcg gctcatccgt cgtcatggcc gtcgtggcta
    22261 tcccaagcac tctaatggga ccctcggcac gcacgatcgg acgggcgagg ggatgcggca
    22321 ccttcgcctt cttccggcgt ttcgtggccg aggtcagatc cacatagaga cgccaggggc
    22381 agacgacata gtcaccttcg agccgcccct cgcccaacgg gccgccgatg tgattgcaga
    22441 ttccagagan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cttcgagccg
    22561 cccctcgccc aacgggccgc cgatgtgatt gcagattcca gagatggctg cgaacgtgcc
    22621 gtccctatag ctgagggcga gcttcgtatt gccgcagaca acttcctgca gtggcttccc
    22681 cttcagttcc tcaaccaacc cgatctctgt ccacctcaca tccggcatgt cacattccta
    22741 tcggcgtccg cggcagggct gcccgtcaca agcgctgctc ttcaagatca tacacatcga
    22801 aataggtgta aacggggcgg tggctcggga catacttcgg aatatagtct tccatatagg
    22861 cctgtaccgc gggagccatc tcggaaagac gcagatcccg catcgtatta ttccattcaa
    22921 tgatcgcctg ccgatcctgc accttgctgt gctccttgag ccagtcgccg agttcctcgt
    22981 cggtcgctcc cgttgcgaca acctccttga actggtccgg actgatcccc gtaaaggtga
    23041 acaacaacgc ggcgagcgaa caaggccaat agttatattc cccgttgatg cccaggagga
    23101 atgcccggca cttatcggca caacgagcgc cgataacata ccctcccagc atttccctcg
    23161 ggctgcgagg ataggtcttg gtgagatctt tcgcaagcgt gcgaacctgc tccagattct
    23221 tcatgtggtc gctccttttc ctgatccggc acgcgagggc ttggcgaacg tgatgccggc
    23281 cttttggact tcctgatagg gcagcagcca gaccagcgat tcgtccgaca agtctcccac
    23341 ctggatccgg tttccccagg ggtcgcgaaa atcgcaacgg aaatggggct ccatcttcag
    23401 cttatatttc ttggtgagct tgcgccggac ctctttaatc tgatcggcat cgcgcaccat
    23461 cagtccgaaa tgcttcatgc gatccggctg caacgtcttc acttcgaaga tggccagaaa
    23521 ttgatgctcg cccagtttgc accaggcagc accctctcct ccgcgtaaca tctccaagcc
    23581 gaacacatcc gaataaaact tcacggcctt tttcgcgtcg gtgacttcga tgacgatatg
    23641 gttgcatccg tagacctgaa ccgacatgtc cattgctcct taccgggttg tgtgctcgat
    23701 ggcctggagg aaggtctcgg ccggctgcgc accggacacc acggtttttc cattcaagag
    23761 gaagtacggg acccctctga ttcccaactg gcgaccgcgt gcctcttcct gtcgcaccgc
    23821 ctcgaccccg tcctggctct gcaggaaccg accgaccgct gccccgtcca ggccggcccg
    23881 ggcagcgaga gacaccagca cctcgtccgc tccgatattc agcccgtcgg tgaagtatcc
    23941 atgaaagagt tcttccatcg cggcgtcctg acgaccctgt tgctgagcaa accagatcag
    24001 ccgatgggcc tcgaaggtat tcggcgtgcg agcaatccgg tcgaaggcga actcgatccc
    24061 ggcacttacg cctgcgcccg caattcgatt ctgaatcgcc tgcatttcct ccggaccgcc
    24121 gaactttgct tcaaggtata cacgacgatc catgccggcc ttcggcatgg tcggattcag
    24181 ttggaacggg cgccaaaaca ccagagcaga gtcctgtctg cccgtcgatt ccagcgcctg
    24241 ctccaaccgt cgcttcccga cataacacca gggacagacc acgtcggaat agacctcgat
    24301 ggtcagtacc ggcgccgtca tgacaaatgt cccatctttc ccgcttggta gtcctgcacg
    24361 gcctggataa tctcatctcg tgtgttcatc acaaacggac cgtagcgcgc gatcggctcc
    24421 gcaataggct tgccgctcag cacgaggatg gtcgcctcct gtttcgcctc cagaaccaca
    24481 cgctcacctc gttgtccgaa cagcgcgatg tgcacgtcgt ccactgactg agacccgttc
    24541 accaccacct gcccatggag cacgaacagg gacgaattga acccctcggg caagtcgagt
    24601 tccagccggt gccctgccgc caaacggagg tcatacaggt gcaccggact aaacgtcttc
    24661 gccggtcctg ccacaccgcg aaattctccc gcaatgacgc ggagccggcc ggcgcctccg
    24721 ccaagttcca ccatcggaat gtcgctgctc aacaacgtct gataccgcgg cgccgtcatt
    24781 ttgaacgcct tgggcagatt cacccacagt tgaacccctt ccaagagacc gcccttttct
    24841 gcaaattctt tctcgtggag ctcctcgtgc acgacgcctg acgcggccgt catccactgc
    24901 acatcgccgg gcccgataac accgccgctc cccgtcgagt cacgatgcgc cactttcccg
    24961 tgatacatga tcgtcacggt ctcgaagcct cgatgcggat gttcccccac accaagccgg
    25021 cgcgtggtcg gcggaaattc ttcggggccc atatagtcga gcatcaaaaa gggagacagt
    25081 tgctcctcga cccctgcgcc cggaatcata ttgcgcacgg gaaagccgtc tccaaccata
    25141 tgatgggagc ctgcctggta gactccgacg agttcttttg cgttcatggt ttctgttcgc
    25201 atgtcggcct cctttcttac gccatctccc gcaatgcttc tgcgagagcc ttcgttcccc
    25261 gcacgagtga ctgagggcgc tcatacctga ccacgtcctt ggaaaacgcg gtatacatct
    25321 cttgaaacaa tccggccgct tcggtggaaa acccgaatga ggtgaatgtc ggcaccactg
    25381 cgctcaacgg cgccgcctgt acggagacgc ctcgtccgaa aatccggcca agctcagagg
    25441 ccacctcttc aggactgtac tcctctggac ccgctagttc cacaatcgca tggccctcgc
    25501 ctccggcaat caatcgctcc gctgccacac gaccgatgtc ccgggtagat atcatcggca
    25561 tcgacatctt cggcggtata aacgacggca acagaccctc cgccttggcc attccgatac
    25621 cattggccca attctccata aagtaacctg gccgcaagat cgtgacgttg ggcgccacgg
    25681 ccgtgagccg atgttctcca taacgagcgg cgcgaatcgg ccccgtgcct tccggaaggt
    25741 gggcgccaat agaagacagc aggaccacat gcgcaatcct ggtcgccttc acggcctctg
    25801 cggtttggtc tacccgccgc cgttgctcgg ccagccatga cgttgcacca tagttcggcg
    25861 gcaccaggag atagacgccc gatgcgcctt gcaatgcctt cgtcagagcc gcctggtcat
    25921 ccaacgaggc cacggcgact tccgctccct tcgcagccca agtagcaccg tgatcggccg
    25981 accgtaccac tactcgcacc ggctgcttgt gcgtcagcag ggtctgcgcg acgaccgatc
    26041 ccgtatgtcc tgtcgcaccc agtacaacca acatcgttgt cgttcccttc ttttcctgaa
    26101 ttgccccatg acacgaccgc tgaaccgtcg gcatcggccg gctcccatcg ccggcaggag
    26161 aagacggcca cccccacttc gctgcacccc actataatag ttctagttag aacaaagtca
    26221 agtgcaaata tgccggggct ccttcacttt ctgagttgat gcatggacag gctgtaagac
    26281 atgaccacga tagaagaccc accgacgata cgctcgagcc gttacacgag gtggggtcag
    26341 tcagcagcag cgggaggacg agattacgac gacgatcagc ggagcaggga agcaggcgag
    26401 ggtctacaga tcaatgacga aggtgagaga ggctatttcc gactcttgat cgcccgttcg
    26461 acttcgcggc cagcctcacg agccttgatg gactcacggc ggtcgtactg ctttttgcct
    26521 ttggccaggg ccagctccac tttcgcatga ccgcgtttcg aaaagtagat gcggagaggg
    26581 acgagagtga gtcccttgag ctgggttttc ccgatcagtt tattgatctc cttgcgatgc
    26641 agcaggagct tccgtttgcg gagaggatca tgattcatca ggttgccatg cgagtagggg
    26701 ctgatgtggc aatggttgag gtacacctcg ccatcatcga ccgacgcata actgtcctgg
    26761 aggttcacgc ggccctcgcg cagcgatttc acctcggtgc cgcgcaggat cacgccggcc
    26821 tcaaacttct cttcgacaaa ataatcgtgg taggccttcc tatttgtggc caccacctta
    26881 tactgatcgt ctgtatcgtg cgttttggtc atgttgctga ggcgaccgct gtttcactcg
    26941 cgcgcatttt gatatgatgc cgccatgcgc ggacagagca gactcgattc cttcggcact
    27001 attctagcag gtgtcgccca ccggttgggc ctcgagagca aactcttcga ggcgcgtcta
    27061 cgacgccatt ggccagagat tgtgggcgaa ccgatcgcga cccacacgcg ccccgatcaa
    27121 atccgattca agaaacttca cgtctacgtc cataactctg tctggctgca acaactcaca
    27181 ttcctcaagc cggtacttct cgaaaaagtg aatgggatgg ccggggagac gctcgtgacg
    27241 gaaatcgtcc tgcgcatcgg ggagttttcc gccgagcgca aagtgagccc gccggcagcg
    27301 acggttgaag agcctgtccc tcagccgagc gccgaattgc tccaggaaac ggccctgcat
    27361 acgcagaaca ttcaggatcc tgctcttagg gagcaacttg cggccgtcat ggcgcaggcg
    27421 ttgtcgggga taccgccgga ctcgtcgcga cgggcccctt gaacaactgc ctcgaatagg
    27481 ccgccgttgc cgcactgtct ttgcccttgg gcaccggccc gatacggcct tcttcttcct
    27541 gttcgtccaa cttgtagtat ccccgtgccg aaccctcgaa tccgtcccgc accgccggat
    27601 cttcgcccgt cacatactta tgcagcattt caggctccgt ccatccctcg tcagtgaaga
    27661 tgtaaatcgc gatgcgttgg tagcgccctt tcggatcatc tgcggtggtc ggacggatct
    27721 tcatggggcc gaaatttctt gtctccgtcc ccaccttgcg gaatcgttcg attaaggttt
    27781 ggatctcggt gtcgctcgtc caggacggga cgtgcaccgc gacgatgcta ccttcctggg
    27841 agccgatgct gtagggagga atggaacgat ccggccggct cagaaacata ccgccccaga
    27901 tcagagcaaa ggagctgaac agaacgccca gcgccagttt cacgaccgta tccaggggac
    27961 gtttcggagg acggcctgcg gaagtcccgg cggacggcgg ttgctgctgg ttcataacca
    28021 tggtgaagga cgcctgaatc cgccggcgtt cacggagtga ctactcgccc tcttcgggca
    28081 ccccttcttc acgttcgacg agccgcacca cggcgacgac acggtcctca tcaccatcca
    28141 tatcaagcag acgcacgccc tgggtgttgc gtccgatttc acgaatgtcg tcgaccttgc
    28201 agcgcagcac tttcccctgc gcagccatca gcatgatttc gtcctcatcc cggacctgga
    28261 ggaatccaac ggccgcgccg ttccgttccg tagtcttgac gctgatgatg cccttgccgc
    28321 cacgtccctg cacgcgatac tcgttcaccg gcgtgcgctt cccgtaaccg ccttcggtca
    28381 ccgtgagaat cgccgtcgtg gaatcgggcg tgatggtctc catgccgatc acctcatcac
    28441 ccgcctccag agtaatgcct cgcacaccgt gggccgtacg tcccatggcc cgcacatctt
    28501 cttccttgaa acggatggtg atgccctgct tcgtgcctaa cagaatttct cgctgaccat
    28561 cggtaagatc gacaccgatc aatttatcac ccgcatcaag cgagagggcg atgatgccgc
    28621 cctggcgagg attgctgtag gccgacagct ccgtcttctt gatgacgccc tgcttggtcc
    28681 ccatgatgac aaaccggtcg gcgcggtatt ctttcaccgg cagcgtggct gtgactttct
    28741 cgtccttgga gagtgccagc agattgacca acgctttgcc cttggccgcc cggctcgcct
    28801 cggggatttc gtgcacctta agccagtaga ccttgcccgc atcggtaaag aacagcagcg
    28861 agtcatgcgt ggaggccgtg aagagagttt caacgaaatc ctcttccttg atgcccatgg
    28921 caatcttccc tttgccgccc cgccgttgcg cgcgatagag cgtgaccgcg ttccgcttga
    28981 tgtacccggc atgcgaaatc gtcaccacga cttcttcttc ggcgatcaag tcttcgagcg
    29041 tcagttccgc ttcttccttg acgatcttcg tgcggcgttc gtcctgatac ttctctttga
    29101 tctccgtcaa ttcatcccgg atgatcttgc gcaccaacgc ttcactgccg aggactgacc
    29161 gcaggtattc gatggtcttc aacacttcgc gatactcttc caccaacttg tcgcgctcga
    29221 gttgcgtgag ccgctggaga cgcatttcga gaatcgcgtt ggcttggatt tcagacaacc
    29281 ggaactgctg catcaaaccc gcgcgcgcca catcgggcga ctgcgagcgc cggatcaggg
    29341 caatcacggc atccaggttg tccagggcga tcttgagccc ttcgaggata tgggcacgtt
    29401 cttccgcctt gcgcaaatca taggccgtgc gccgcacgac cacttcgcgg cgatgctcga
    29461 caaaggcttc caggatccgc ttgaggttca gcacctccgg ccggttgttg accagcgcca
    29521 gcatgttgac gccgaaggtc gtctgcaact gactgtgttt atacaaattg ttcagcacta
    29581 cgagcggaat ttcattgcgc ttcagttcga tcacgacccg gaccccttcg cgatcggact
    29641 cgtcccggat atcggaaatg ccggtcacgc ggtcttcctg cgccaggtcg gcaatttttt
    29701 cgatcagctt cgacttgttc acctggtagg ggatttcagt gatgatgagc cgctcgcgat
    29761 cggtgcgttc atcggtctcg atcgccacct tggcccggac cgtgagaagc ccgcgaccgg
    29821 tctcataggc atccttgatg ccggacgtgc cgtagataaa tccggcagtg gggaagtccg
    29881 gtccgggaat ttttttcatc agctgcgcaa tcgtcacctc cgggttctcc agcagcagga
    29941 gcaacccctc aatgacttcg cccagattgt gggtgggaat gttggtcgcg taaccgacgg
    30001 caatcccgcc cgcgccgttg accagcagat tcggcacccg cgagggcaac accagcggct
    30061 caactcgcga ctcgtcgtag ttggggccga agtcgacggt ctctttttcg atatccgcca
    30121 gcaactcttc ggccagtttg gtcaaccggg cttcggtata ccgcatggcc gctgccgcgt
    30181 cgccgtccat cgaaccatag ttgccctgac cgtccaccag catgtagcgc atgttgaagt
    30241 tctgcgccat gcgcaccagg gtatcgtaga tcgccgagtc accatgggga tggtaattac
    30301 ccatgatctc gccgacgatc tttgccgact tacggtatgg ccgattggcg gccaatccca
    30361 tctcgttcat gccatgcaaa atgcgacggt ggaccggttt caacccgtcc cgcacgtcgg
    30421 gcaacgcgcg accgacgatc acgctcatcg cgtagtcaag gtacgacgag cgcatctcgt
    30481 cttcgatcgc aatttggcct aatcgttcat ctggcggcat ctaccctcca cggatgctga
    30541 caatgtctct tggcttcgtt ctcggctcga gaaggtcctc aacgtacccc tgagtctacg
    30601 cgtccggtcc tctcttgcct gcggccttgc cgaagcccat ttttgagcat ccttcgaatt
    30661 cagaaaacca aagtggttgt gtctacgatc acgaccgact atacgtccaa attccggact
    30721 tccagcgcat gttgctggat aaagttacgc cggggctcca cttcatcgcc catgagaatc
    30781 gtaaaaattt catcgacgcc cgtcatgtct tcgaggttga cgcgcaacag cgtgcgcttc
    30841 tcgggatcca tcgtggtttc ccagagctgg cccggattca tttcgccgag acctttatac
    30901 cgttgaatgt tcaagccctg tttgccttcc tccaggatgg cctgtaccag ctcggccgag
    30961 ccgttcttga gaacctcacc ttccttgatc ttgatcttgt acggaggttt ccccaacccg
    31021 atcgaggagg gagcctgttt ctgcaattca cggaagtcag ccgatccgac cagctcgtgc
    31081 gtcacctgca actcataggc gattccgcca gtggcgacct tacagaccag tttgctcgat
    31141 tgatgctctt catcttccag gatctcgatc gtcggttcgg ccttcgggta gaccagtgcc
    31201 agtgtggcct tcacgtgatc aacgaccgtg cgcagcgacg ccggatcctt caagacttct
    31261 cgcgtcaacg ccggttcatc gacgaacgcc cgtagcatgt tggcttcatg atgcttcttg
    31321 ttgaccttat gcagaaggct ttcgaaggca atcagcttct tgagaatcgg cagcaaccgc
    31381 cggcccgtga cgaactcgcg gccgttctcg agcgcgactt ccaccccctc cacggcaagg
    31441 tcggcgagat actcgttcat cgccggctcg tccttcaggt accgttccgt cttgcctttc
    31501 ttcactttaa agaggggagg ctgcgcgatg taaatgtagc cccgctccaa cagctcaggc
    31561 atctgccgga agaagaaggt gagcaacagc gtgcggatgt ggctgccgtc cacgtcggcg
    31621 tcggtcatca gcacaatctt gtggtaccgc gtcctggcga tgtcgaacgc gtccttgtcc
    31681 gccttgtcgg aatcttcttt ctttcggccg atgcccgttc ccagcgccag aatcagcgtg
    31741 cggatttcgt cgctgctcag catcttgtcg aaccgcgctt tttccacatt gaggatcttg
    31801 cccttgagcg ggaggatggc ctggaacttc cggtcgcggc cctgtttggc cgatccaccg
    31861 gcggaatctc cctcgacaat gaacagttcg ctcaaggccg gatctttttc ggaacagtcg
    31921 gccagcttgc cgggcaagga tccgccgtcg agagcgcttt ttcgccggat gagatctttc
    31981 gctttccgcg cggcctcgcg ggcgcgcgcc gcatcgatgg ccttgccgat gattttgcga
    32041 gcgaccgggg gattctcttc gaagtaggtg ccgagtgcgt cgttgactgc cgcctcgaca
    32101 atacccttca cttcgctatt gccgagtttc gccttggtct gtccctcgaa ttgcgggttc
    32161 cgcactttga cgctcaccac cgccgtcaac ccttcccgca catcgtcgcc actcaacgat
    32221 tccgtatcct tcttcaggag gtcgttggcg ttggcgtaac tattgatcgt gcgggtcaac
    32281 gcggctttga accccaccag atgcgtgccg ccttccttcg tgttgatatt gttcgcgaag
    32341 gaaaacaggt tttcggcgta gccgtcgttg ta
//
LOCUS       DAOQDS010000047        31771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_485_length_31771_cov_19.470015, whole genome shotgun sequence.
ACCESSION   DAOQDS010000047 DAOQDS010000000
VERSION     DAOQDS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 31771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31771
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_485_length_31771_cov_19.470015"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PK589_12505"
     CDS             complement(<1..905)
                     /locus_tag="PK589_12505"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNJ20474.1"
                     /translation="MTQTLPIPFYMLCGSLGAGKTTLLMRLLEYWNSQGHKVGVLMNE
                     AGEVSIDGPRAGTIADQVLNLAGGCICCDTKDDLAWSIAQLVQDYESTIIVLECSGMA
                     DPAEVVDAVTDAYVSRMVKLERIFAMLHPVPLPDTGMAELVTRNAIRYADDLILNKHD
                     LYIPGHWEQFRETVTRYNPYGRLWETNHARLDVAALLAAPQTRTNPTNVSFGKLPSAE
                     SRRDARASHHPMVTTVRLPKPLNRTKFMEWTKSIPEGMERAKGFFRFEGEPELQEFQF
                     FPPRTSTIAPVMLLDEPDHVLVLIGR"
     gene            complement(1139..2080)
                     /locus_tag="PK589_12510"
     CDS             complement(1139..2080)
                     /locus_tag="PK589_12510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNJ20475.1"
                     /translation="MLPHTYHELRSQLRIALAVNAVIVVAEFAGGFLTNSIGLIGDAG
                     HNLVDQGSLFLALYAHVLTARPATDSRTFGYHRAGIVAAFLNSFILLLTAGGITLVSI
                     ERLMAPVPVPGGWVMLIALISFAANLSIALLLQHGAKDDLNIRSAFWHMLSDAWVSLG
                     VVVSGGIIMVTGWSVLDPLVSLFVVLAIVRGAWPLFKESLEVLLESTPPGVSPALVAA
                     TIESIPGVKNVHDLHIWAVEPRLIMMTTHVQVDGGDQALTTDLLQTIRNRVTSEFKIK
                     HLTIQLETECCHPEAVHCDLSKLHDQHSHAEFLHTHH"
     gene            complement(2204..3991)
                     /gene="ftsH"
                     /locus_tag="PK589_12515"
     CDS             complement(2204..3991)
                     /gene="ftsH"
                     /locus_tag="PK589_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNJ20476.1"
                     /translation="MDPKQRQFSIWYMFIALWVLILIQTFLPSLFNPTEIPYSEFKSA
                     VDANKVTEVTVSPQIIHGKMKEDKVFHTIRIEDPDLLRNLAQHQVKVTGMIESTLFRD
                     LLSWILPIVLFFGVWWFLLRRMGQSQGFMTVGQSKAKIYVENEVKVTFADVAGVDEAK
                     QELEEIIEFLKTPEKFRRLGGKIPKGILLVGPPGTGKTLLAKAVAGEAGVPFFSISGS
                     EFVEMFVGVGAARVRDLFEQAKGKAPCIIFLDELDALGKARGVGPMAHEEREQTLNQL
                     LVEMDGFDSRVGVILVAATNRPEILDPALLRAGRFDRQVLVDRPDKIGRLAILKVHAR
                     SITLANQADLETIAAMTPGFVGADLANLLNEAALLAVRRGKDTVSLSELQEAVERVIG
                     GLEKKNRVLNKMERARVAHHEVGHALMALSIPGGDAVHKISIIPRGIAALGYTMQLPT
                     EDRFLMTVSELKNRIAILLGGRAAEEVIYGEVSTGAQDDLRKATDIAKSMIKSYGMSE
                     KLGQVSLERDRQSVFLQTGPSQTPGDYSEHTSREIDCEVRLLIDEQYERARDLIKAQE
                     ATLRHAAHVLLEKETITGEELKALAAPPQ"
     gene            complement(4109..4975)
                     /locus_tag="PK589_12520"
     CDS             complement(4109..4975)
                     /locus_tag="PK589_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20477.1"
                     /translation="MIRAFTIRLIGTGLLVLLSSMSAAFTPEPPESELESKAHYNDPL
                     PTTVLVRVVAHRSLVLGHEVGGARVTMTDVATGQLLASGLQQGEPGDQTQIMRTPHLM
                     DEPVYSSRPAAAFSATLELTRPTLVEITAEGPLAYPQALQRASTTVLLIPGHDLTNDG
                     IVLHLYGYLVQVEHPKPGEPLIAKEDVMLRASVRTLSGALVRPHSDWDSRKMHIYAEL
                     LIGDRIVERLQMFYAGEKSRFEAPFFVPLSKDAPDGITLRVVAADVATGNFGVSSAQF
                     PVLSERLRPKKN"
     gene            complement(4972..6990)
                     /gene="metG"
                     /locus_tag="PK589_12525"
     CDS             complement(4972..6990)
                     /gene="metG"
                     /locus_tag="PK589_12525"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNJ20478.1"
                     /translation="MSTRDTFYITTPIYYVNDVPHIGHAYTTAAADVLARYWRLRGRD
                     VFFLTGLDEHGQKVQQAAAKAGIDPQAHCDRLAPQFTNLWDRLNISHNAFIRTTDKPH
                     QSVVQRYLQQLFDKQLIYKADYTGWYCTFDERFWTEKDVVGGLCPDCKRPIEQLSEHN
                     YFFKMGQYQEQLQRHILEHPDFIRPESRRNEVLGFLQTQKLGDLSISRPKSRLSWGIE
                     LPFDKDYVTYVWFDALVNYISALEYLPQGQPAGLRYWPADVHLVGKDILTTHAVYWST
                     MLMALEQPLPNTIFAHGWWTVDGEKMSKSRGNVVDPNKMVDQFGTDAFRYFLLREVPF
                     GQDGDFSHSALVTTVNAKLANGIGNLLSRTLTMIERSCAGVIPERGPAVLPELEQRIE
                     ELAAQLPGKTETGFNALQFRDVLLMIEELNGCCDEYIDKSAPWKLAKQPETQPQLHTV
                     LNTSARALRLLALLLYPFMPKAAAQMIEQLGLSLDLSRPLAAEAGRWDAPLAGSKIQK
                     GASLFPRIESKPQGAKPVSDTPASPQPTAAAPSAAPAAPQSPAPATTPAAAPAAAQPA
                     QITIDDFMKVQLKAAKVLAAERVPKSEKLLKLQVSLGTEQRQIVAGIGKKYEPEALVG
                     KTIVIVANLKPAKLMGIESQGMVLAAGDSEVRGLATFVEDVEPGTKVK"
     gene            complement(7096..8127)
                     /gene="holB"
                     /locus_tag="PK589_12530"
     CDS             complement(7096..8127)
                     /gene="holB"
                     /locus_tag="PK589_12530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNJ20479.1"
                     /translation="MPFADIIGHEHAKTLLRSAILQNRLAHAYLFHGDDRIGKRLLAW
                     RLAQTLLCETVSDGRQPDACGTCRACQQVDARTHPDFLVIEPDQEMANPQIKIESIRD
                     IEHQMIYRPLIGNRKICLIDEADRMTIGAANALLKTLEEPPDHSLFILVSSRPYALPA
                     TIRSRCQALRLTAPAQTQVEAAVILKRELPPADAHFLALLSDGQLGRALECDLEQART
                     TQKEFAAIFSAKGLQSFSTVLAAAEALAKGDRGPEAFDWLLRWLRDILLIAVGAGSDH
                     ILNLDQRAGMQALAAHINIDDLLDLIGDLEKMERQAHRNLNVQIALETILLRVRQLLT
                     PSGTAERSR"
     gene            complement(8114..8836)
                     /gene="tmk"
                     /locus_tag="PK589_12535"
     CDS             complement(8114..8836)
                     /gene="tmk"
                     /locus_tag="PK589_12535"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNJ20480.1"
                     /translation="MMKHYRPPRGLFITLEGIEGCGKSTQARLLGEYLRSQGHVTVET
                     REPGGTPLAEKVRSVLLDRADEPVAPETEAFLVLAARRQHVAQVIEPALAQGAIVLCD
                     RFSDSTLAYQGYARGLNVPLLERLNRLATHAVMPHLTLLFDLPVATGLARRRSASETN
                     RLDRESLRFHQKVRAGFLDLAKRHPARIKVIPSRASKETVARAVARIVAPALNRIHRA
                     DHQTAKPRATSSHTTQVRHALR"
     gene            8971..9645
                     /locus_tag="PK589_12540"
     CDS             8971..9645
                     /locus_tag="PK589_12540"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNJ20481.1"
                     /translation="MVQASLKRYFLTGLLVMIPIWGTILILKTLFVSLDGILGDAAAR
                     LVTPGYYVPGLGIIALILLIFMTGLFAANFIGRQVVRQWESLLNRVPVVRGIYSTIKS
                     MMDILSFAERESYRRVVLIQFPKNGHYCFAFVTGVTKGEMQQLSPDPLVHVYVPTSPN
                     PTSGYFLLVPEREVIAVDITVEEAMKLIVSGGLYTPTQPSGAGSQAGGKTWTPIKQPD
                     AGVQVG"
     gene            9697..11310
                     /gene="glmU"
                     /locus_tag="PK589_12545"
     CDS             9697..11310
                     /gene="glmU"
                     /locus_tag="PK589_12545"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNJ20482.1"
                     /translation="MTHASHDNASSSSIPGLGVIIMAAGLGKRMKSALAKVLHPVAGR
                     PMVLYVLDIACGLAEQGVAVVVGHQGADVRKVVEAVGGQVAVAEQTKQLGTGHAVLQA
                     RPVFGDAAHRKPTRYLILNGDTPLLTEATVRELLAMHDAQGAAVTLLTAVLDDASGYG
                     RVIRHRREEWLQGAADNRVQSIVEDKDASHAERSVREINVGTYVVDGEFLFPALDKLD
                     PRNAQGEYYLTDIVQMAVQQGRTVSALRLRDIDEGLGINSRVQLAEAEGVIRRRIRER
                     WLEAGVTMLDPASAWIDADVTIGRDTLLYPNVTLEGRTVIGENTVVHSGTRITDCTIG
                     QRVEILDHCIFRESQVEDDSHLGPFVHLRPGVVVRRKGKVGNFVEMKKTELGEGSKAN
                     HLSYLGDAMIGQGVNIGAGTITCNYDGYKKFHTVVGDGVFIGSDVQLVAPVTVGQGSV
                     IAAGATVTQDVPADALVIARVPQVTREGWAARRRALQSGQVPPPAPKPVPPVKARASA
                     TPAKAASKPKPRTRAAKKKQPAVQRSKRR"
     gene            11320..13152
                     /gene="glmS"
                     /locus_tag="PK589_12550"
     CDS             11320..13152
                     /gene="glmS"
                     /locus_tag="PK589_12550"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNJ20483.1"
                     /translation="MCGIVGYVGNQDAVPILLNGLSKLEYRGYDSAGVAIQRGEKIDI
                     RRSVGKLINLQNSLAQKAIAGMCGIGHTRWATHGKPSEQNAHPHRSENCVLVHNGIIE
                     NYVELKQRLIKDGYKFQSETDTEVVAHLIDTHIKKDKLHLADAVRATAREIRGSYAIA
                     VISEHEPGLLVAARSGCPLVIGRTAEASFVGSDVMAMLSHTRDVTFLEEGDVAEVTAG
                     GVTFTDLDGRAVTRKKTKVTWDASAAEKSGYPHFMLKEIHEQPQTILDTIRGRYSYES
                     GEADLPDIGLTPKQFADVGRIWIVACGTSWHAGLVGKYLLEEMVRTPVQVDIGSEFRY
                     RDPLIEKNDLFITISQSGETADTLAAAREAKQKGARVVSIVNVVGSTLARESDGVLYT
                     HCGPEIGVASTKAFTSQLAALYMLALHLGRVRGVLTAADGKAWLDRLVTVPALVKHVL
                     GREAEILAIAKRYYKKPDFLYLARGINFPIALEGALKLKEISYIHAEGYAAGEMKHGP
                     IALIDKDMPVVVLAPRDRLYEKTVSNLMEVKARRAPVIAFVAEGERELGKIADAVFTI
                     PDVHPLLSPILFTIPLQLLAYHIAVLRGEDVDQPRNLAKSVTVE"
     gene            13270..13563
                     /gene="gatC"
                     /locus_tag="PK589_12555"
     CDS             13270..13563
                     /gene="gatC"
                     /locus_tag="PK589_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNJ20484.1"
                     /translation="MEITKQEVEKVAKLARLALSEAETTAFSQQLNQIVGYVQKLKSF
                     STEDVEPTSTVLGQTNVFRPDQVQASLSPEQALGNAPDAEAQCFRVPKIIQES"
     gene            13589..13954
                     /locus_tag="PK589_12560"
     CDS             13589..13954
                     /locus_tag="PK589_12560"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNJ20485.1"
                     /translation="MFRQMLRAKIHRATVTEACLEYEGSLTVDEDLLDAAGILPYEAI
                     VCSNLNNGERFMTYAMKGKRGGGEIVLNGPTARKAAVGDQIIIFCYEYYSDEEIKRHK
                     PKIILVDGKNRIARKVAKR"
     gene            13954..15429
                     /gene="gatA"
                     /locus_tag="PK589_12565"
     CDS             13954..15429
                     /gene="gatA"
                     /locus_tag="PK589_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNJ20486.1"
                     /translation="MALTVLHKMTLAELQRKFTAGDVSATEIVRAYFLRVAHVEPKVN
                     AYLTQCKEAAVAQAERLDQSLKGWRKTTPLMAMPLAVKDNICTEGVRTTCASRMLESF
                     VPPYDATVVAKLRAQNYLLVGKTNLDEFAMGSSTENSAFGASRNPWNIQTVPGGSSGG
                     SAAAVAADECVASLGSDTGGSIRQPAAFCGVVGLKPTYGRVSRYGLVAFASSLDQIGP
                     ITKDVTDAAILLGAIAGHDPRDSTSANVPVPDYLKALKRKDLKRLKVGVPAEYFADGL
                     DPEVEQAVRTAIEGLRELGADIREIKLPTTDAAVATYYVIATAEASSNLARYDGVKFG
                     LRAGDSKDLLDMYLRTRAEGFGPEVKRRIMLGTYVLSAGYYDAYYGKAQAVRTLIRRE
                     FDAAFETVDLIVTPVTPTTAFKFGEKSQDPLQMYLSDIYTISANLAGLPAISLPCGFS
                     KAGMPIGLQLIGRPFEEDTLLRGAHAYEQATNWRSKKPLVR"
     gene            15448..15849
                     /locus_tag="PK589_12570"
     CDS             15448..15849
                     /locus_tag="PK589_12570"
                     /inference="COORDINATES: protein motif:HMM:NF017876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HNJ20487.1"
                     /translation="MIVEVAAILVAIAFAVLVGYLVPLLIQVRKTAAEAEQLVTKMNT
                     DLPVLVTELRAMSQNLNDLTEQARGGVEHAAVLLHAVGEVGESVNQVHSLVRGSGGNL
                     LANVASVVAGLRAAKQVVTERLKEGGHHNGG"
     gene            15839..16159
                     /locus_tag="PK589_12575"
     CDS             15839..16159
                     /locus_tag="PK589_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNJ20488.1"
                     /translation="MADNHGPSSAAVLLGFLSGAALGAVAAILLTPRTGPESREMLRG
                     YARRAEDGLRDLVGEAGERFEGAVEEGRDFIESKKSVLREAFDAGREAMRREREQFTK
                     GEQS"
     gene            16156..17589
                     /gene="gatB"
                     /locus_tag="PK589_12580"
     CDS             16156..17589
                     /gene="gatB"
                     /locus_tag="PK589_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNJ20489.1"
                     /translation="MSVAYEVVIGVEVHAQLRTQSKLFCPCGTTFGRTANSQTCPVCL
                     GLPGSLPVINEAAVEMAVRAGLAMNGTIGVHNRFARKNYFYPDLPKGYQISQYEAPIC
                     EHGWIEIAAGGTRKRVRIRRAHLEEDAGKNLHEAGSGMSLVDLNRAGTPLLEIVTEPD
                     LSSSEEVVAYLKALRELLMYLDVCDGNMEEGSFRCEPNLSLRPVGQKAFGTKVELKNI
                     NSFKFVKDAVDYEIKRQTKVLNEGGKIYQETRLWNHERGETAVMRSKEEAHDYRYFPD
                     PDLVPLEIAADWIEQLREGLPELASTKQQRFVAEYGIPEYDAGILTSSKALSVYFDAC
                     VKVYPHPKTVSNWVMGELLRELNHAGIQADASPVTPERLVELLTMVDQGAVSLKVARD
                     IFPEMYASGKPPAQIVQDKGLTQVSDEGALVSIIDEVLKKNPAQVAQFKEGKQQVLGF
                     LVGQVMKASGGKANPGKVNELLKKTLG"
     gene            17665..18951
                     /gene="eno"
                     /locus_tag="PK589_12585"
     CDS             17665..18951
                     /gene="eno"
                     /locus_tag="PK589_12585"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNJ20490.1"
                     /translation="MSGIRNVKARQIIDSRGNPTVEVEVLLESGAHGRAAVPSGASTG
                     EKEAIELRDGDKKRWMGKGVSKAVANVSKAIAPRLLGMEALDQAAVDHEMIALDGTKT
                     KGKLGANAILGVSLAVAKAAANETGQPLYRYLGGTNARVLPVPLMNIINGGAHADNRL
                     DLQEFMIMPVGAPRFSDALRMATEVFHTLKSLLKKKGLNTAVGDEGGFAPDLQSNEEA
                     LALIMEAIEAAGYRPGQDIALALDCAASELYEKGRYRLEAEKNPERSSEEMVSYYGKL
                     LDRYPILSIEDGLSELDWKGWKILTEKLGKRVQLVGDDIFVTNVEIFAKGIAEGIGNS
                     ILIKLNQIGTLTETLDAIELAKRSGYTAIISHRSGETEDTTIADVAVATNSGLIKTGS
                     LSRTDRVAKYNQLLRIEDELGAAAVYRGRTAVPSRT"
     gene            18957..19319
                     /locus_tag="PK589_12590"
     CDS             18957..19319
                     /locus_tag="PK589_12590"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNJ20491.1"
                     /translation="MIIKPNRGRDWLDWQRKLFSAGKWVGLGALFLMMGALLFGEMGI
                     SRYLHLREHAEQLDHELADLQRLNGELRADLDRVQYDSTRIEELARERLGYVRKGETV
                     YQLAPLGEKERLPTVKTP"
     gene            19316..20200
                     /gene="era"
                     /locus_tag="PK589_12595"
     CDS             19316..20200
                     /gene="era"
                     /locus_tag="PK589_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNJ20492.1"
                     /translation="MKFGSVAIIGRSNVGKSTLLNRLLKEKIAIVSDKPQTTRTRILG
                     VAHVEGAQIVFLDTPGFHEPHHLLNRRMVRTTLETFDDADVLYVVVEATSQPGPGDLA
                     VIEHVKQAVAKQARPVVLVINKVDLVNKSRLLPLMEQYLRIFPWTEMVPVSAQTADNT
                     DRLLSVTVSLLDEGEATYNEDMITDQSMRTLAAELIREKILQQTYEEIPHSVAVEIEE
                     FVETKKLIKIGAVVIVEKESQKGILIGKHGERLKSVGTAAREDMERLFGTKVFVKLWV
                     KVRESWREDEQTLAELGY"
     gene            20307..21644
                     /gene="mgtE"
                     /locus_tag="PK589_12600"
     CDS             20307..21644
                     /gene="mgtE"
                     /locus_tag="PK589_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNJ20493.1"
                     /translation="MRLVSIQRLLRRGAITNLAKMLARMHPADIANVIDHLSSQKEKR
                     EIFELVRGDVKRGQVLSELDGESITLVLSDLLPSDSAWLLKDLGSDDIAHILSVIPND
                     RAKEILALMRTEDSTEIADLLKYPKGTAGGIMTTEFFALFEDATAQEAIRRLQQATDA
                     EMVFYIYVTDKEDRLVGVLSLRQLLTVPPATPLKNIMTRDLISVAVDMDQEEVSRQVA
                     SYNLLAIPVVEKDGKLVGIITVDDVVDVIREEATEDMLKMAGAVEEDAMFKSSSMAAA
                     RVRLPWLFTNLVGSLLSGMLLWMFRYTIQEVVAIVSFIPVIAAMGGNVGLQSSTLIIR
                     GLATGLVELTDVWKIFFREAKIAFLMGIACSLMLTVVGWLWHQVFLGMVVGVSLITAF
                     LVSTSMATVMPIVLKRMGVDPAVAAGPFVTTANDITGIAIYLTLATIFLEQIR"
     gene            21699..22457
                     /gene="recO"
                     /locus_tag="PK589_12605"
     CDS             21699..22457
                     /gene="recO"
                     /locus_tag="PK589_12605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNJ20494.1"
                     /translation="MPLVKTAAIVLHSRKWGDADRIVTCYTLRLGKLRGVARGARRLK
                     SRLGGMIEPFTLCQLDLFEKPGDSLYRISQVALDEPFAKLRSDLGLMTAAGRMVNLVS
                     AVMAEGDAEPRVFEMLESGLRTLLESRDAAWTTVLFQIRLLGITGFRPQTEQCATCGQ
                     VHRTPLPQFSPLAGGMVCERCASRQPFRCTALSRGSVAFLQQALHMQPALLNRLQAAG
                     QVRAEVESVIESYVTVVAGRRLPAVDFLTSGGPA"
     gene            22516..22872
                     /locus_tag="PK589_12610"
     CDS             22516..22872
                     /locus_tag="PK589_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNJ20495.1"
                     /translation="MGETVLEPVDMEWVEKGVLSITWSDGHKARYPVRYLRQHCPCAA
                     CTDEWTGELRLKPDDVPMVIMLQDIQPVGRYAFQFTWSDGHDTGIYSYSFLRRLCQCD
                     VCQPVKPVEPRSRRLL"
     gene            22920..23381
                     /locus_tag="PK589_12615"
     CDS             22920..23381
                     /locus_tag="PK589_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20496.1"
                     /translation="MNVSKLASVGLWLLIGLISVACSGLPPIDQQKRLVQAGELKIQQ
                     LTPRAFAETWGDPTYTHQQFTHFFGMPDGQLIPQARMALGESPQGWETGLAAGDAFFM
                     AYADRGYYLVFLEGVLVYHEAMSAEKVHAVGKTWKFESQFKTRLESSPGLK"
     gene            23438..24922
                     /gene="glgA"
                     /locus_tag="PK589_12620"
     CDS             23438..24922
                     /gene="glgA"
                     /locus_tag="PK589_12620"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNJ20497.1"
                     /translation="MVASEVVPLAKTGGLADVVGALAVEFAKLGHDVCVMLPAYRQVD
                     ELGYEMTEGVRLSVPAGPSVMDVRVQEVFAPPLHVQGPGRLRVFVVRHDPYFSRSGLY
                     QEAGRDYLDNLDRFVCFCRTVMEMCIHLSEHEGWQTDLLHLHDWQAALCAVYLRTLYQ
                     GRPSLRRIRSVLTIHNLGYQGVFPAAQFSSTGLPLHLFTPAALEFYGQINVLKGGLVS
                     ADLLTTVSPTYSLEIQSSEYGCGLEGVITERKSVLSGIVNGIDTESWNPATDQYLVAP
                     YHASDLAGKARCKKALQRELGLRPLKCPLLGVIARLTSQKGIDLVIDVLPELMELDVQ
                     VVILGTGEPHYEQQVGELAQRYPGKIAVRNVFDEGLAHRIEAGADMFVMPSRYEPCGL
                     SQLYSLRYGTVPIVRKTGGLADTVVAYTPRAFKESRATGFSFTDTSSASLLTCLLLAL
                     SVYRKKAEWQRLVKTGMAQDLSWSRSAAIYLQLFRDVISRAGDR"
     gene            complement(24960..25448)
                     /locus_tag="PK589_12625"
     CDS             complement(24960..25448)
                     /locus_tag="PK589_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20498.1"
                     /translation="MRYHTLRQIILLLAGLTAVGCQSNPAVTSVRYDNVRFMDVWSTY
                     THCLSAEDSQVALQDSTKLRVVSRAQTTRSTLDTILPTSLKNMVSQPSSRLAVDVHAM
                     AASCSLHAGNLAMSAGELDVARSQFREVLQGHAESDYAYYTAQARERLTQLELTLQAA
                     LR"
     gene            26092..27726
                     /gene="aepX"
                     /locus_tag="PK589_12630"
     CDS             26092..27726
                     /gene="aepX"
                     /locus_tag="PK589_12630"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HNJ20499.1"
                     /translation="MNSRQPLTKTRQFRNLLLSEQLEFICEAHNGLSAKIVEEAGFPG
                     IWASGLSISAQFGVRDNNEASWTQVLEDLEFMSDATNIPILLDGDTGYGNFNNMQRLI
                     RKLEQRNIAAVCIEDKLFPKTNSFLKGDAQPMADMHEFCGKIKAGKDAQTDPDFCVIA
                     RVEAFICGWGLTEALRRAEAYHQAGADGILIHSALSVPDEILAFKREWGNRCPVVIVP
                     TKYYSTPTDVFRQHGFSMVIWANHMLRAAVSVMQKTARALKQSENLLSIEDKVAPVSE
                     IFRLQNAAELLEAEERYLPRGAEGTSAVVLAASRGEELGELTVEQPKTMVKIQGTPIL
                     SHIVDAYNAVGMKDILVVRGYKKEAVNLPNLTYIDNADYSQTGELASLSLALQSRKGV
                     FQPTIVSYGDVLFNKYIPQALCQEPDDCVIFVDSNWRDQTTYARLGGFVECTIPNSRK
                     AFNAKVYLKQLGNRIPTDATHGVWMGFLKLSSSAACDINNLLVEMLREPENRKAGIPH
                     LLQELLRREYPIRVLYTVGHWLDINSLDDVVQAGNF"
     gene            27835..28965
                     /gene="aepY"
                     /locus_tag="PK589_12635"
     CDS             27835..28965
                     /gene="aepY"
                     /locus_tag="PK589_12635"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HNJ20500.1"
                     /translation="MIDPRKFVECLQSNSVEFFTGVPDSLLKEFCACLEQVPQAGRHV
                     ISANEGGAVALALGYHLATGKVPLVYLQNSGLGNIINPLLSLADAEVYSIPLLLVIGW
                     RGETGVHDEPQHKKQGRVMLAMLDAMEIPYSILGPEVNEAEGALSDALAYVQKHNAPF
                     ALVIKKGTFGAFTGDKRKTEIFELTREAAIQQVLEHLDERDVVVSTTGMASREVYEYR
                     MNKGQGHERDFLTVGGMGHASQIALGVALQRPSRSVYCLDGDGAVLMHMGALPITGTL
                     KPENFKHIVFNNGAHDSVGGQPTVGLEIEILEIAKACGYQLVVQAQTPQEVQAGLREL
                     KRSKVPCLLEIRIRRGARKDLGRPATTPVENKSAFMAFVQGG"
     gene            complement(29143..29754)
                     /locus_tag="PK589_12640"
     CDS             complement(29143..29754)
                     /locus_tag="PK589_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNJ20501.1"
                     /translation="MAQIAITSQETADNLADQRTGAGIVVLSSSMQLLHMNRQAAELS
                     KLINMAEKGGTPAKAAQGVLPSALTELCTEILKALQVRTEAKDWEQFEVKKIAGNPNQ
                     PVLLRGFGLPDRGGVQYARLVVTLEELGRRQQLNTDQAKERFQLTNREQSVVENLAKG
                     WTNKEIANALKITEQTVKEHIKHIMRKTNSTTRTGVLVQIFKS"
     gene            30232..30933
                     /locus_tag="PK589_12645"
     CDS             30232..30933
                     /locus_tag="PK589_12645"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNJ20502.1"
                     /translation="MKAKVVLSLMVLAGNCLNVAFAGPFDQAPKATLVAGAPAAAPPS
                     GMRHGGDGAEKSSLTVTPDYIMGPEDVLEITVWKNADLSKQVQVRPDGRISLPLIGDV
                     SAVGRTAAQLTEEISARLKSYMENPTVSILVREVNSYQIYVLGEVNAPGKYPLKSKTT
                     LLQAITIAHGFTQVAARNKIVVFRFGKDGEGLNKIKASYDDIVLRDGSDQNIELKPGD
                     QIVVPSETMVVLPSR"
     gene            30995..31618
                     /locus_tag="PK589_12650"
     CDS             30995..31618
                     /locus_tag="PK589_12650"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNJ20503.1"
                     /translation="MKTFGCRWLRGGVCIVFSVLLTLPGCWIGNEQIDFNVTYIPPNE
                     FLLGPEDVLVVNVWRNQELSREVIIRPDGKISMPLVGDVQAAGMTSNVLAKRIADGLA
                     EFMSNPTVSVQVKEVNSYYVYVLGEVSKPGKVPLKSYATVLQGISLAGGFTTFASRNK
                     IHVLRVVPNGQGQPKQVQIPVPYEDILQGKNSEGNFFLKAGDVIVVP"
BASE COUNT         6513 a         8155 c        10124 g         6979 t
ORIGIN      
        1 cgtccgatca acaccaggac atggtccggt tcatcgagca gcatgaccgg agcaatggtg
       61 ctggtgcgag ggggaaagaa ctgaaactcc tgtagctccg gttctccttc aaaccgaaaa
      121 aatcccttcg cgcgttccat tccctccggt atcgacttcg tccattccat gaactttgtg
      181 cggttcagcg gtttcggtaa ccgtacggtg gtcaccatcg ggtgatgtga cgcccgggcg
      241 tcgcgcctgc tctctgccga gggcaatttc ccaaacgata cattcgttgg attggtccgt
      301 gtctggggcg cggcgaggag ggcggctacg tcgagcctgg cgtgattggt ttcccacagg
      361 cgaccgtagg gattgtagcg ggtgacggtc tcgcgaaact gttcccaatg gccggggatg
      421 tagaggtcgt gtttgttcag gatcaaatca tctgcatacc ggatggcatt ccgcgtgacg
      481 agctcggcca tgccggtatc gggcagggga acggggtgca gcatggcaaa aatccgttcg
      541 agtttcacca tacgggagac ataggcgtcc gtcaccgcat ctaccacttc tgccggatcg
      601 gccatgccgg aacattccag aacaatgatg gtggactcat agtcctgcac cagctgagcg
      661 atgctccagg cgagatcgtc tttcgtatcg caacagatgc agccgccggc caggttcagg
      721 acctggtctg cgatggtgcc cgctcgtggg ccgtcgatgc tgacctctcc tgcctcgttc
      781 atgagcacgc cgactttgtg cccctggctg ttccaatatt cgagcagtcg catgagcagg
      841 gtggtcttgc cggcgccgag tgagccgcac agcatataga aggggatggg cagtgtctgt
      901 gtcatgcggc ccattgtagc cgctgaggtc ctgtggcggc taggtggcgc tttcatgcgc
      961 agctttggca caaagatatc tcgcgatcac aggcagatgg ttgggcaggt ttcccggcgg
     1021 atggtcaaaa agtccgccag cgcggccgca gcgagcgagg aggcgaggcg tacccttgcg
     1081 gtacgttgag cctttgagcg atgcgagaac aatgctggcg gattttttca ccatccgcct
     1141 agtgatgcgt gtgcagaaat tccgcgtgcg aatgctggtc gtggagtttg gagaggtcgc
     1201 aatggacggc ttcggggtgg cagcattcgg tttcgagttg aatggtcaga tgtttgattt
     1261 tgaattcact ggtgacccgg ttgcggattg tttggaggag gtcggtcgtt aaggcctgat
     1321 cgccgccgtc gacctgtaca tgggtggtca tcatgatgag gcgtggctcg accgcccaga
     1381 tatgcaggtc gtggacgttc tttaccccgg ggatggattc aatggttgct gcgaccagcg
     1441 ccgggcttac gccgggcggg gtggactcca gtaagacctc aagcgattct ttaaagagtg
     1501 gccaggctcc cctgactatg gccagcacca cgaacagact caccaggggg tccaacacgg
     1561 accagccggt gaccatgata atgccgccgc tcacgacgac accgagcgac acccaggcat
     1621 cgctcagcat gtgccagaag gcgctgcgga tattgaggtc atctttcgcg ccgtgttgga
     1681 gcaagagcgc gatggaaagg ttcgcggcaa agctgatcaa ggcgatgagc atgacccatc
     1741 ctcccggaac ggggacggga gccatgaggc gttcgatact gacaagggtg atgccgccgg
     1801 cggtgaggag caggatgaag gaattgagga acgccgcgac gattcctgcc cggtgatagc
     1861 cgaaggtgcg gctgtcggtg gcgggacggg ccgtcagcac atgggcatag agcgcgagga
     1921 agagagagcc ttgatcgacg aggttatgcc ccgcgtctcc gatcaggccg atgctgttcg
     1981 tgagaaagcc gccggcaaac tccgcgacga cgatgacagc attgacggcc agggcgatgc
     2041 ggagttgcga gcggagttca tggtaggtgt ggggaagcat cgacttcagt gtctcacgag
     2101 aggcggagga gggcaagccg ttgtgtggtc ttcaagtgac tgcggaccag tgcgcgagat
     2161 ctcttggatt ctcaagggag agaggcgagg caaggaggcg gcgttactgg gggggtgccg
     2221 cgagggcttt gagttcctcg ccggtaattg tttctttctc aagcaagacg tgagcagcat
     2281 gccgcagcgt ggcttcctgg gctttgatga gatcgcgtgc ccgttcgtat tgctcgtcga
     2341 tcaagagtcg aacttcgcaa tcgatttctc tcgacgtgtg ctcactgtaa tcgccgggcg
     2401 tttgagacgg accggtttgc aaaaagacgg attgccggtc ccgctccagg ctgacctgcc
     2461 caagtttttc gctcatgccg tatgacttga tcatgctctt ggcgatatcg gtggccttgc
     2521 gcagatcgtc ctgagcgccg gtggagacct cgccgtagat gacttcctcc gcggcgcgtc
     2581 cgcccagcag aatggcaatg cggttcttga gctcggacac cgtcatgaga aagcggtctt
     2641 cggtcgggag ctgcatggtg tagccgagcg cggcaatacc tcgagggata atggagattt
     2701 tgtggaccgc atcgccgccc gggatagaca gggccatcag cgcatggccg acctcatgat
     2761 gcgccacgcg ggcgcgctcc attttgttca gcacccgatt cttcttttcc agcccgccga
     2821 tgacgcgctc gaccgcttcc tgtaattcgg acagactcac cgtgtccttg ccgcgccgta
     2881 cggccagcag ggctgcttcg ttcaggagat tggccaggtc ggcgccgacg aagcctggcg
     2941 tcatagcggc gatagtttcg aggtcggcct gattggcgag ggtgatgctg cgcgcgtgga
     3001 ctttcagaat cgcgaggcgg ccgatcttgt cgggccggtc caccagcacc tggcgatcga
     3061 agcggcctgc gcgaagcagt gcggggtcca aaatctcagg acggttggta gcggccacga
     3121 ggatgacgcc gacgcgggag tcgaagccat ccatttcgac cagcagctgg ttgagcgtct
     3181 gttcccgttc ctcatgggcc atcggtccga ccccgcgcgc tttccccagc gcgtcgagct
     3241 catcgaggaa gatgatgcag ggcgctttgc ccttggcctg ttcgaacagg tcgcggacac
     3301 gagcggcgcc gaccccgacg aacatctcca cgaattcaga cccgctgatc gaaaaaaacg
     3361 gcacaccggc ttctccggcc acggccttcg cgagtagcgt cttgcctgtg cccggggggc
     3421 cgacgagcag aatgcccttg ggaattttcc cgccgaggcg ccggaatttt tccggcgtct
     3481 tgagaaattc gatgatctct tcgagttcct gctttgcctc atcgacgccc gccacatcgg
     3541 cgaaggtgac cttcacttcg ttctcgacat aaattttggc tttgctttgc ccgaccgtca
     3601 tgaagccctg gctctgaccc atgcggcgga gcagaaacca ccagaccccg aagaacagca
     3661 cgatcggaag aatccacgac aagagatccc ggaacagagt gctctcgatc atgcccgtga
     3721 ccttgacctg gtgttgggcc aggtttcgca gcaggtccgg gtcttcgatg cgaatggtat
     3781 ggaagacttt gtcttccttc atcttgccgt gaatgatttg cggcgagacg gtcacctcgg
     3841 tgaccttgtt cgcgtccacg gcggatttga attcgctgta cgggatttcg gtggggttga
     3901 acagggacgg gagaaaggtc tggatcagga tcaggaccca gagggcgatg aacatgtacc
     3961 agatggagaa ttgtcgttgt ttgggatcca tcgtgaggaa tctacgatcg tgtaatcagg
     4021 gactccactg gtgatgagcg gcagtcggca gatcatgact gcagcgcctt gtcagcacat
     4081 catgaaacga ccgattcagt caatggggtc aattcttctt gggtctcagc cgttcggaga
     4141 gcaccggaaa ttgcgccgag gagaccccga agttgcccgt cgcaacatcg gctgcgacga
     4201 ctcgcaacgt gatgccatcg ggggcgtctt tggagagtgg aacaaaaaac ggggcttcga
     4261 agcggctctt ctctccggcg tagaacatct gcaggcgctc gacgatgcgg tccccgatca
     4321 gcagttcggc atagatgtgc atcttccggg agtcccagtc gctatgtggc cggaccaggg
     4381 cgccggagag tgtgcgcacc gacgcgcgca gcatcacgtc ttccttggcg atcaggggtt
     4441 cgccgggttt gggatgttcg acttgcacca ggtagccgta gaggtgcagc acgatgccgt
     4501 cgttcgtcag atcgtggccg ggaatcagca gcacggtggt gctcgcccgc tgcaatgcct
     4561 ggggataggc cagcgggccc tcggcggtga tttccaccag ggtcgggcgg gtcaattcaa
     4621 gtgtggcgct gaacgcggcg gccgggcggc tgctgtagac cggctcatcc atgagatggg
     4681 gcgtgcgcat gatctgggtc tggtcgcccg gttcgccttg ctgcagcccc gaggccagca
     4741 actggccggt cgccacatcc gtcatggtga cgcgcgcgcc gccgacttcg tggcccagca
     4801 ccagagagcg gtgcgcgacg acgcgcacga gcacggtggt agggagcgga tcgttgtagt
     4861 gggctttgct ctcgagctcg gattcggggg gctccggcgt aaacgcggcg ctcatgctgc
     4921 tcaacagcac gagaaggccg gtgccgatca gccggatggt gaaggcgcga atcatttcac
     4981 tttggtgccg ggctccacgt cttcgacgaa ggtggcgagt cctcgtactt cgctgtcgcc
     5041 cgccgcgagg accatgccct gcgactcgat gcccatgagc ttcgccggtt tgaggttcgc
     5101 cacgatgacg atggttttgc cgaccagggc ttccggttcg tatttcttgc cgatgccggc
     5161 gacgatctgc cgttgctcgg tgccgaggct gacctggagc ttgagcaatt tctcggattt
     5221 cggcacgcgt tcggctgcca agaccttggc cgctttgagt tgcaccttca tgaagtcatc
     5281 aatggtgatc tgcgccggtt gcgcggcggc cggagcggca gccggtgtag ttgccggagc
     5341 aggggattgt ggtgcagcgg gcgctgctga aggggcagcg gcagttggtt gcggagatgc
     5401 gggagtatca ctcacgggtt tggctccttg tggtttggat tcaatgcggg ggaataacga
     5461 ggcacctttt tgaatcttgc tgccggccag cggcgcgtcc caacggccgg cctccgccgc
     5521 gagcgggcgg gacaggtcta gcgacaatcc cagttgctcg atcatctgcg cggcggcctt
     5581 tggcataaag ggatagagta gcaaggccag cagtcgaagg gcgcgggcgg aggtgttcag
     5641 gaccgtatgt aattgtggtt gtgtctccgg ctgcttggcc aatttccacg gcgcgctctt
     5701 gtcgatgtat tcgtcacagc agccgttgag ttcttcaatc atcaacagca cgtcccggaa
     5761 ctggagggcg ttgaaacccg tttcggtttt gccggggagt tgtgcggcca attcttcaat
     5821 acgttgttcc agttccggca gcaccgccgg gcctcgttcg gggatgacgc cggcgcagga
     5881 ccgttcgatc atggtgagcg tacggctcag caggttgccg atgccgtttg cgagtttggc
     5941 gttgacggtc gtgaccagcg cgctgtggga aaaatcgccg tcctgtccga agggcacttc
     6001 gcgcaggaga aagtaacgga aggcatcggt gccgaattgg tcgaccatct tgttcggatc
     6061 gacgacgttg ccgcggctct tggacatctt ctcgccatcc accgtccacc agccatgggc
     6121 gaagatcgtg ttggggagcg gctgctcgag cgccatcaac atggtggacc agtacacggc
     6181 gtgcgtggtg agaatatcct tcccgaccag gtggacgtcg gcgggccagt aacgcaaacc
     6241 ggccggttgt ccctgcggca ggtattcgag tgccgagatg taattgacca gcgcatcgaa
     6301 ccagacgtag gtgacatagt ccttgtcgaa gggaagttcg atgccccagg agagtcgcga
     6361 tttgggtcgg gagatcgaga ggtcgcccag cttttgagtt tgcaaaaagc ccaggacttc
     6421 gttgcggcgc gattccggtc ggatgaagtc cggatgctcc aggatgtgcc gctgcaactg
     6481 ttcctgatac tggcccatct tgaagaaata attgtgctcg ctcagttgtt cgatcgggcg
     6541 cttgcagtcc gggcagagcc cgccgacgac atctttttcc gtccagaacc gttcgtcgaa
     6601 cgtgcaatac catcccgtgt aatctgcctt gtaaatcaac tgcttatcga agagctgttg
     6661 aaggtatcgt tgaaccacgg attggtgggg tttgtcggtg gtgcggatga aggcgttgtg
     6721 ggaaatgttc aaccgatccc agaggtttgt aaactgcggt gcgaggcggt cgcagtgcgc
     6781 ttggggatcg atgccggcct tcgcggcggc ctgctgcact ttctgtccgt gttcgtcgag
     6841 accggtcagg aaaaatacgt cgcgtccacg caatcgccag tagcgcgcga gcacatcggc
     6901 ggcggcggtg gtgtaggcat gaccgatgtg cggcacgtcg ttgacgtagt agatcggtgt
     6961 cgtgatgtag aaggtgtcgc gagtgctcat ggtttgtggg gtgaagatag caagatgccg
     7021 atggtcaaag ctagctggct gttgagaagg ccgccagcgg cgttctcagt tgcgcgcctc
     7081 cctgcgatga ctcagttatc gtgaccgttc ggcggtgcct gacggggtca ggagttgacg
     7141 gactctgagc aggatggttt cgagcgcaat ctgcacgttc aggttccggt gcgcctgtcg
     7201 ttccattttt tcgaggtcgc cgatgagatc cagcaagtcg tcgatgttga tgtgcgcggc
     7261 cagcgcctgc atcccggctc gttggtccag gttcaggata tggtcggagc cggcaccgac
     7321 ggcgatgaga agaatgtcgc gcaaccaccg aagcagccag tcaaatgctt ccggtccccg
     7381 gtcccctttg gccagcgctt ccgcggcagc caggacggtc gagaacgact gaagtccctt
     7441 ggcggagaag atggccgcaa attccttttg ggtggtgcgc gcctgttcaa gatcgcattc
     7501 caatgcgcgc ccgagttggc cgtcgctgag taaggcgagg aagtgcgcat cggcgggagg
     7561 cagctcgcgt ttcaaaatga cggcggcttc gacttgcgtt tgtgccggtg cggtgagtcg
     7621 tagcgcctgg cagcgtgaac gaatggtggc cggcaaggcg tagggccgac tcgacacgag
     7681 aatgaacaga ctgtggtctg gtggttcttc cagcgttttg agcagggcat tcgccgcgcc
     7741 gatggtcatg cggtctgcct cgtcgatcag gcagattttg cgattgccga tcagcggacg
     7801 gtagatcatt tgatgttcga tgtctcgaat ggactcaatc ttaatttgag gattggccat
     7861 ttcctgatcc ggttcgatca ccagaaaatc cgggtgcgtc ctggcatcca cctgctgaca
     7921 agcccggcag gtgccgcagg cgtccggctg gcggccatcg gaaaccgtct cgcagagcag
     7981 ggtttgcgcc aaacgccaag cgaggagacg tttgccgatg cgatcatcgc catggaagag
     8041 atacgcatgc gccaaccggt tctgcaggat cgccgatcgc agcagggtct tggcgtgctc
     8101 gtgtccgata atatcagcga agggcatggc ggacctgtgt ggtgtgggag gatgtggcgc
     8161 gcggcttagc cgtctggtgg tcggcgcgat ggattcgatt cagggccggt gcgacgatcc
     8221 gggccacggc gcgggcgacg gtttctttgg atgcacgcga cggaatcact ttgatacgtg
     8281 caggatgacg ctttgccagg tcgaggaagc cggcgcgaac tttctggtgg aaccggagcg
     8341 attcgcgatc gagccgattc gtttcgctgg cagatcgtcg ccgtgccagt cctgtcgcca
     8401 cgggaagatc gaagagcagc gtgaggtgag gcatcaccgc gtgagtcgca aggcggttga
     8461 gtcgttccag cagcggcaca ttcagcccgc gcgcatagcc ctgataggcg agcgttgaat
     8521 cgctgaaccg gtcacagagg acgatggcgc cctgtgccag ggcgggctcg atcacctggg
     8581 ccacatgttg gcgtcgcgcg gcgagcacga gaaacgcttc ggtttcgggc gccaccggtt
     8641 cgtcggcacg atcgagcagc actgatcgga ctttttccgc cagcggcgtt ccacccggtt
     8701 cacgggtctc gacggtgacg tgtccctggc ttcgaaggta ttcgccgagg agcctggcct
     8761 gggtggattt tccacaccct tcgatgccct ccagcgtaat gaacagccct cgcggcggac
     8821 ggtagtgttt catcatgaat cgaacctgaa aagttctggg agaaagaggg cggatcctat
     8881 tccaagtgat tgaaaatagc aagaaaaggc ttgcgacgac cggcaaaaga ccagtatcat
     8941 gacgctctac ccgggacccc tcagccacgc atggtacaag cttcattaaa acgctatttt
     9001 ctcaccggtt tgctggtgat gatccccatc tggggcacca tcctgattct gaagacgctg
     9061 ttcgtcagtc tggatgggat tctcggcgac gccgccgcac ggttggtaac tccaggctac
     9121 tacgtgcccg gtctgggaat tatcgcgctc atcctgctca ttttcatgac cggattgttt
     9181 gcggcgaatt ttatcggccg ccaggtcgtg cggcagtggg agagtttgct caatcgtgta
     9241 ccggtggtcc gcggaattta ttcgaccatt aagtcgatga tggacattct gtcgttcgcg
     9301 gagcgcgaga gttatcgccg tgtggtgttg attcagtttc cgaagaacgg gcattactgc
     9361 tttgcctttg tgaccggtgt gacgaaaggg gaaatgcaac agctttctcc ggacccgctc
     9421 gtccatgtct atgtgccgac ctcgcccaat cccacgtcgg gctattttct gctggtgcct
     9481 gagcgcgaag tgatcgcggt ggatatcacg gtcgaagagg ccatgaagct gatcgtgtcg
     9541 ggaggtctct ataccccgac gcagccgtct ggtgccgggt cgcaggccgg tggaaaaacc
     9601 tggaccccga ttaagcaacc cgatgcaggc gtgcaggtcg gttgatcgcg ataccgccga
     9661 acgagccctg tgtcgattac gtatggtgag aaacccatga ctcacgcatc ccacgacaat
     9721 gcctcgtctt cctccattcc cgggctcggc gtcatcatca tggcggcggg ccttggcaag
     9781 cgcatgaaat cggccttggc caaagtgctg catccggtgg ccggtcgacc gatggtgctg
     9841 tacgtgctgg acattgcctg tggcctggct gagcaggggg tggcggtagt ggtggggcat
     9901 cagggggccg atgtgcgcaa ggttgtcgaa gcggtcggcg ggcaggtggc ggtcgcggag
     9961 cagacgaagc agttgggaac cggccatgcg gtgctgcaag cgcgtccggt attcggcgat
    10021 gctgcacatc gcaaaccgac ccgctatctg attttgaacg gcgacacgcc gttgttgacg
    10081 gaagcgacag tgcgggaact gctcgcgatg catgatgcac aaggtgcggc cgtgacgctg
    10141 ttgaccgccg tgctcgatga tgcctcgggc tatgggcggg tgattcgcca tcgtcgtgaa
    10201 gaatggctgc agggtgccgc cgacaatcgg gtgcagagca tcgtggaaga caaggatgcg
    10261 tctcacgctg agcgaagcgt gcgtgaaatt aacgtgggta cgtatgttgt cgacggggag
    10321 tttctctttc cggccctcga caaactcgat ccgcgaaatg cgcagggcga atactatctc
    10381 accgacatcg tgcagatggc cgtgcagcag gggcgaaccg tctcggcatt acgtctgcgg
    10441 gacatcgacg aagggctggg gatcaacagc cgcgtgcagt tggcggaggc ggaaggcgtc
    10501 attcgccgcc ggatccggga gcgttggctg gaagccggcg tgacgatgct cgacccagcc
    10561 tccgcctgga tcgatgccga cgtgaccatc gggcgcgaca cgctgctgta tcccaatgta
    10621 acgctcgaag gccggacggt gatcggggag aatacggtgg tgcactccgg gacccggatt
    10681 acggactgca ccatcggcca gcgggtggag attctggacc attgtatttt tcgtgaatcg
    10741 caggttgagg acgactccca tcttggaccc ttcgtccatt tgcggcctgg tgtcgtggtg
    10801 cggcggaagg ggaaggtcgg caactttgtc gagatgaaga agacggagct cggcgaaggc
    10861 tcgaaggcca accatttgag ttatctcggc gatgccatga tcggccaggg cgtgaacatc
    10921 ggcgccggta cgatcacgtg caactacgac gggtacaaga aatttcacac cgtcgtcgga
    10981 gacggcgtgt tcatcggcag cgatgtgcag ttggtcgcgc cggtaacggt cggtcagggc
    11041 tccgtgattg ccgccggggc aaccgtgacg caagacgtgc ccgccgatgc cctcgtgatc
    11101 gcgcgagtgc cccaggtgac gcgtgaggga tgggctgcca ggcggcgggc cttgcagagc
    11161 gggcaggtcc cgcctcctgc gccgaagcct gtgcccccgg tgaaggcccg cgcgagtgca
    11221 acacccgcga aggcggcatc gaagccaaag ccgcggaccc gtgccgccaa gaagaagcaa
    11281 ccggcagttc aacgttcgaa acggaggtaa cgacacccta tgtgtggcat tgtcggctac
    11341 gtaggaaatc aggatgcagt cccgattttg ttgaatgggt tgtcgaagct ggagtatcga
    11401 gggtacgact cggccggcgt ggcgatccag cgcggagaaa agatcgacat ccggcgcagt
    11461 gtcgggaagt tgatcaatct tcaaaactcg ctggcccaga aggcgatcgc cgggatgtgc
    11521 ggcatcggcc atacccgctg ggcgacgcac ggaaagccct ccgagcagaa tgcgcatccg
    11581 caccgctccg agaactgcgt gctcgtgcac aatggcatca tcgaaaacta cgtagagctg
    11641 aagcagcgcc tcatcaaaga cggatacaag tttcagtccg agaccgatac ggaagtcgtc
    11701 gcgcatctca tcgataccca catcaaaaaa gacaagctgc acctggctga tgcggtccgc
    11761 gcgacggcga gggagattcg cgggagttat gccatcgccg tgatctcgga gcatgagccc
    11821 gggttgctcg tcgcggcccg atccggttgc ccgctggtca tcggtcgtac ggcggaggcc
    11881 tcatttgtcg gctcggacgt catggcgatg ttgtctcaca ccagggacgt gacgttcctc
    11941 gaagagggcg atgtcgccga ggtgacggcc gggggtgtga ccttcacgga cctcgacggg
    12001 cgggcggtca cgcgcaagaa aaccaaagtt acctgggatg cgtctgcggc tgaaaaaagc
    12061 ggctatcccc atttcatgct gaaggaaatt cacgagcagc ctcaaaccat tctggatacg
    12121 attcgaggac gctactcgta cgagagcggc gaggcggatt tgccggatat cggcctgacg
    12181 ccgaagcagt tcgccgacgt ggggcgcatc tggatcgtgg cgtgcggcac cagctggcat
    12241 gccggcctgg tgggaaagta tttgttggag gaaatggttc gtacccccgt gcaggtcgat
    12301 atcgggagtg aattccggta tcgcgacccg ttgatcgaaa aaaacgacct gttcatcacg
    12361 atctcacaat cgggggaaac cgctgacaca ttggcggccg cccgggaagc caaacagaag
    12421 ggcgcgcgcg tcgtgtcgat cgtcaacgtc gtgggcagta ccctcgcccg tgaatcggac
    12481 ggcgtgttgt acacccactg tggccctgag atcggcgtcg cgtcgacgaa ggcgtttacc
    12541 agtcagttgg cggcgctcta catgttggcc ttgcacctcg gtcgggtacg gggtgtctta
    12601 accgccgcgg atggcaaggc ctggctggat cgcctcgtca cggtgccggc actggtgaag
    12661 catgtgctgg gtcgtgaagc ggagattctg gcgattgcca agcggtacta taagaagccg
    12721 gacttcttgt atctggcccg cggcatcaac tttcccattg cgctcgaagg cgcactgaag
    12781 ttgaaagaaa tttcctatat ccacgccgaa gggtatgcgg cgggggaaat gaagcacggc
    12841 cccatcgcgc tgatcgataa agatatgccg gtggtggtat tggcgcccag ggaccggtta
    12901 tatgagaaga cggtcagtaa cctcatggag gtgaaggcgc gccgggctcc ggtgattgct
    12961 tttgtggcgg aaggcgaacg cgagttgggc aagatcgccg acgcggtgtt caccattcct
    13021 gatgtccatc cgttgctgtc gcccattttg ttcacgatcc cattgcagtt actggcgtat
    13081 cacattgccg tgctgcgagg cgaagatgtg gatcagccgc gaaacctggc caagagcgtg
    13141 acggtggaat gaggtgcgaa ggatggactc ccgtgctcgc gcaacgcgcg gcctcggaag
    13201 gccctcgttg gacgcgcgca attggagccc cttccttcgc accataccgg tttaaatgag
    13261 gggtgtagag tggagatcac gaaacaggaa gtcgagaaag tggccaagct ggcgcgcctg
    13321 gcgttgagcg aggcggaaac gacggcgttt tcacaacagc tgaaccagat cgtgggctat
    13381 gttcagaaac tcaagtcctt ttccacggaa gatgtggagc cgacctcgac cgtgttgggg
    13441 cagaccaatg tgtttcgtcc ggaccaggtg caggcatctc tgtcgccgga gcaggcgctg
    13501 gggaatgccc ccgatgcgga ggcgcagtgt ttccgggtgc cgaaaatcat tcaagaatct
    13561 taagtgacgt aaggagtacg agccgactat gtttcgacaa atgttgcggg cgaagataca
    13621 tcgagcgacg gtgaccgaag cctgtcttga atatgaaggc agtctcacgg tggatgagga
    13681 tctgttggat gcggcaggca ttcttccgta cgaagcgatc gtctgttcca atctcaacaa
    13741 tggcgaacgg tttatgacgt acgccatgaa gggaaagcgg ggcgggggcg agatcgtgct
    13801 gaacggcccc actgcccgca aggccgcggt gggtgatcag atcattattt tctgttacga
    13861 atattatagc gacgaggaga tcaagcggca taagccgaag atcatcttgg tggacggaaa
    13921 aaaccggatt gcccgtaagg ttgcgaagcg ctgatggcat tgacggtcct acataaaatg
    13981 acgctggcgg agttgcagcg gaaatttacc gccggcgacg tgagcgcgac ggaaatcgtg
    14041 cgcgcctact ttctgcgggt ggcgcatgtc gaacccaagg tcaatgcgta tctcacacag
    14101 tgtaaggagg cggctgttgc gcaggccgaa cggcttgatc agtccttgaa aggctggcgc
    14161 aaaacgacgc ctctgatggc catgccgctg gcggtgaagg acaacatctg caccgaaggg
    14221 gtacgcacga cctgcgcgtc ccggatgttg gaatcgttcg tgccgcccta tgatgcaacg
    14281 gttgttgcca aattgcgtgc gcaaaactat ctgctggtcg gtaagacgaa tctggacgaa
    14341 ttcgcgatgg gatcctccac cgaaaattcg gcgttcgggg ccagccgtaa tccctggaac
    14401 atccagactg ttcccggcgg gtcgagcggc gggtcggcgg cagctgtcgc ggcggatgaa
    14461 tgtgtggcgt ccttgggctc ggacaccggt ggatctattc ggcagccggc ggcgttttgc
    14521 ggcgtcgtcg ggctgaagcc gacctatggc cgggtctcgc ggtacgggtt ggtggcgttc
    14581 gcatcctctc tcgatcagat tggccccatc acgaaggatg tcacggatgc ggccatctta
    14641 ttgggcgcga tcgcaggcca tgatccacgg gattcgacgt cggcgaacgt gccggttcct
    14701 gattatctca aggcgctcaa gcggaaagat ctgaaacggt tgaaggtcgg cgtccccgcc
    14761 gaatacttcg ccgacggtct cgaccctgaa gtggaacagg ccgtgcgcac agccattgaa
    14821 gggttgcggg aactgggcgc cgacattcgc gagatcaaat tgccgaccac ggatgccgca
    14881 gtggcgacct actacgtcat tgcgaccgca gaggccagtt cgaatctcgc ccgttacgac
    14941 ggggtgaaat tcggcctgcg ggccggcgac agcaaggatc tgctcgatat gtacctcagg
    15001 acgagagccg aagggttcgg gccggaagtg aaacgccgga tcatgctggg gacctatgtg
    15061 ttgagcgccg gatactacga tgcctactac ggaaaggcgc aggcggtgcg gacattgatc
    15121 cgccgggaat ttgacgcggc cttcgagacg gttgatctga tcgtgactcc ggtgaccccg
    15181 accacggcct tcaagttcgg ggagaagtcg caggatccgt tacagatgta tttgtccgac
    15241 atctatacga tctcggcgaa tctggccggt cttcccgcca tctcgttgcc ctgtggattc
    15301 agtaaagcgg gaatgccgat cggactgcag ttgatcggtc ggccgtttga agaggacacc
    15361 ctgttgcgcg gggcccatgc ctacgagcag gcgacaaact ggcggtccaa aaagccgttg
    15421 gtgcggtaag ccgggaaggg agttcccatg attgtggaag tcgctgctat tctggtcgcg
    15481 atcgcatttg cggtcctggt cggatacctc gttccgcttt tgattcaggt ccgcaagacc
    15541 gctgcggaag cggagcagct tgtgacgaag atgaataccg acctgccggt cctggtgacg
    15601 gaattgcggg ccatgagtca aaacctgaac gatctgaccg aacaggcgcg cggtggggtg
    15661 gagcatgcgg ccgtgttact gcatgccgtg ggcgaagtcg gcgaatcagt caatcaggtg
    15721 catagtttgg tgcgaggttc cggggggaac ttgctggcca atgtggcgag cgtggtggcg
    15781 gggcttcgcg cagcaaagca agtggtcacg gaacgcttga aagagggagg acatcacaat
    15841 ggcggataat cacggtccat cgtcggcggc ggttctgttg gggtttttga gcggtgcggc
    15901 attaggcgca gtggcggcga tcttgttgac gccccgaacc ggaccggagt cgcgtgagat
    15961 gttgcgcggg tatgcgcgac gtgcggaaga cgggttgcgg gatttggtcg gcgaggcggg
    16021 cgaacggttt gaaggcgccg tcgaagaggg acgcgatttt atcgaatcga agaaatccgt
    16081 cttgcgggag gcctttgatg cagggcgcga agccatgcgg cgggagcgag agcagttcac
    16141 caagggagag cagagttgag cgtcgcctac gaagtcgtca tcggtgtgga ggtgcatgcg
    16201 cagttgcgca cgcagtccaa gctgttctgc ccgtgcggga ctacgtttgg ccggaccgcc
    16261 aattcccaga catgccctgt ctgcctgggg ttgcccggaa gcctgccggt gatcaacgag
    16321 gcagccgtgg agatggcggt gcgcgccgga ttggcgatga acgggacgat cggcgtgcac
    16381 aatcgcttcg cccggaaaaa ctatttttat cccgacttgc ccaagggcta tcagatttcc
    16441 cagtacgaag caccgatttg tgagcatggg tggatcgaaa tcgctgccgg cgggacccgg
    16501 aaacgcgtac ggatccggcg tgctcatttg gaagaggatg ccggaaagaa tctgcatgaa
    16561 gccggaagcg gcatgagcct ggtcgatctc aaccgcgccg ggacgccgct gctcgagatc
    16621 gtgaccgaac cggacctgag ttcctccgaa gaagtcgtgg cgtatctcaa agcgctgcgt
    16681 gaactcttga tgtatctcga cgtgtgcgat gggaacatgg aggaaggcag ctttcgctgc
    16741 gaaccgaatc tctctttgcg gccggtcggc cagaaggcgt tcggcacgaa ggtggagttg
    16801 aagaacatca attccttcaa gttcgtgaaa gacgcggtgg attacgaaat caagcggcag
    16861 accaaggtgc tgaacgaggg gggcaagatc tatcaggaaa cccggctttg gaatcacgag
    16921 cgcggtgaaa cggcggtgat gcgcagcaaa gaggaagcgc atgactaccg gtattttcca
    16981 gacccggatc tcgtcccgct ggagattgcg gccgattgga tcgaacaatt gcgcgaggga
    17041 ttgccggagt tggcctcgac gaagcaacaa cgctttgtcg cggagtatgg catccctgaa
    17101 tacgacgcgg ggattctgac atccagcaaa gccctctccg tgtattttga tgcgtgcgtc
    17161 aaggtgtatc cccatcccaa gacggtcagc aattgggtga tgggcgaatt gctgcgtgaa
    17221 ttgaatcatg ccggcattca ggctgatgcg tcacccgtga cgccggaacg gctggtcgaa
    17281 ttgctcacga tggtcgatca aggcgctgtg agtttaaagg tggccaggga catcttcccg
    17341 gagatgtatg cctctggcaa accgccggcg cagatcgtcc aggacaaggg gcttacgcag
    17401 gtatcggacg aaggggcctt ggtgtcaatc attgatgaag tcttgaagaa aaaccctgct
    17461 caggtggcgc aatttaagga aggcaagcag caggtgctgg gctttctcgt gggacaggtg
    17521 atgaaggcat ccggcgggaa ggccaatccc ggcaaagtga atgagttgtt gaaaaaaacc
    17581 ttgggctgag gctgggatca ggtagcggga aatgagacga cagcagtcat tgttgagtgc
    17641 gagtacctgg aggagacaac gagcatgagc ggaatcagga acgtgaaggc gcggcagatt
    17701 attgattcac gaggcaaccc cacggtcgaa gtcgaagtgc tgttggaaag cggggcgcat
    17761 gggcgggcgg cggtgccctc gggagcctcg acgggtgaaa aggaagcgat cgagttgcgg
    17821 gatggcgaca agaagcgttg gatgggaaag ggcgtgtcca aggcggttgc gaatgtgagc
    17881 aaagccattg cgccgcggtt gttgggaatg gaagcgttgg atcaggccgc cgtcgatcac
    17941 gaaatgatcg ccctggacgg cacgaagacc aaggggaaac tcggtgcgaa tgccattctc
    18001 ggcgtgtccc tggccgtggc caaggctgcc gcgaatgaaa ccgggcaacc cttgtatcgc
    18061 tatctcggcg ggacgaacgc gcgggtgttg ccggtaccgc tcatgaatat catcaacggt
    18121 ggcgcgcatg ccgacaatcg gttggacctg caggaattca tgatcatgcc ggtgggcgcg
    18181 cctcgtttca gcgacgcgtt gcgcatggca acggaagtgt tccacacgct gaagtccctt
    18241 ctcaagaaaa agggattgaa cacggcggtg ggggatgagg ggggcttcgc gccggatctg
    18301 caatcgaacg aagaggcatt ggccttgatc atggaagcga ttgaagcggc cgggtatcgt
    18361 ccaggccagg acattgcgct ggccttggat tgtgccgcga gtgaactcta tgaaaaggga
    18421 cgctatcggt tggaagcgga gaagaatccc gagcggtctt ccgaggaaat ggtctcctat
    18481 tacggcaagt tgctggaccg ctacccgatc ctttcgatcg aagacggttt gagtgaattg
    18541 gattggaagg ggtggaagat tctgacggaa aaactcggca agcgcgtgca gctggtcggc
    18601 gacgatattt ttgtgaccaa tgtcgaaatc tttgccaaag gcatcgccga ggggatcggc
    18661 aactcgatcc tgatcaagct caaccagatc ggcaccttga cggagacgtt ggatgccatc
    18721 gaactggcca agcggtcagg ctacacggcc atcatttcgc atcgctcggg cgaaacggaa
    18781 gacacgacga tcgcggacgt ggcggtggcg accaatagtg gattgatcaa gacgggctcg
    18841 ctatccagaa cggatcgcgt cgccaaatac aatcaacttc ttcgtattga ggatgagctg
    18901 ggtgctgcgg ctgtctatcg gggccgcacg gctgttccct cgaggaccta acgactgtga
    18961 tcattaagcc gaaccgtggt cgcgactggt tggattggca gcggaagctc ttttctgccg
    19021 ggaagtgggt agggctcggc gcgctgttcc tgatgatggg cgcgctgttg ttcggggaaa
    19081 tgggtatttc ccgctatctg catttgcgtg agcatgcgga acagttggat cacgaactgg
    19141 ctgatttgca gcggttgaat ggcgaactgc gcgcggacct ggaccgcgtg cagtatgact
    19201 cgactcgcat cgaggagctg gctcgcgagc ggttgggcta tgtgcgaaaa ggggaaactg
    19261 tgtatcaact tgcgccgctt ggagaaaagg aacggctccc gaccgtgaaa acaccatgaa
    19321 gtttggcagt gtggccatta tcgggcgttc gaatgtcggg aagtctaccc tcctgaatcg
    19381 tctgctgaag gaaaaaatcg ccatcgtctc cgacaagccg caaaccacca ggacacggat
    19441 tctgggtgtg gcgcatgtgg aaggcgccca gatcgttttt ctcgacacgc cggggtttca
    19501 tgagccgcat catctattga accgtcgtat ggtgcggacg acgttggaga ccttcgacga
    19561 tgccgatgtg ctgtatgtgg tcgtcgaagc cacgtcccag ccgggccctg gtgatctggc
    19621 ggtgattgaa catgtgaagc aggcggtcgc caaacaggcg cggcccgtgg tactcgtgat
    19681 caacaaagtc gatctggtga ataagtcgcg gctgcttcct ctcatggagc agtatctgcg
    19741 cattttcccc tggacggaaa tggtgccggt ctcggcgcag acggccgaca ataccgaccg
    19801 gctcttgtcg gtcacggtgt cgctgctgga cgaaggggaa gccacctaca atgaggatat
    19861 gatcacggat caatccatgc gaactctcgc ggcggagctc atccgcgaaa agattctgca
    19921 acagacttac gaagagatcc cgcactccgt ggcggtcgag atcgaagagt ttgtggaaac
    19981 gaaaaaattg atcaagatcg gggccgtggt gattgtcgaa aaggaatccc agaaagggat
    20041 tctgatcggc aaacacggcg agcgcctcaa gtcagtgggg acggcagcgc gggaagatat
    20101 ggaacggctg ttcgggacga aagtgtttgt aaaattgtgg gtcaaggtgc gcgaatcgtg
    20161 gcgagaggac gagcaaaccc tcgcggagtt aggttattaa cgacctcacc attcaaccgc
    20221 cggaaccggc gcgactcccg gagaaggagc cgcttgggaa agatctgtct cgcgaggccg
    20281 ctggtcacgg gcaagcgaag tccgatgtgc gcctggtctc catccaacga ctcctgcggc
    20341 gcggggccat taccaacctg gcgaaaatgc tcgcgcgtat gcatccggca gacattgcca
    20401 atgtgatcga tcacctctcc tcgcagaaag aaaaacggga aatctttgag ttggtgcggg
    20461 gagatgtgaa gcgcgggcaa gtgctgagcg aattggatgg cgagagcatc acgttggtgc
    20521 tgtccgatct gctgccgtcc gattccgcct ggttgttgaa agacctcggt tcagacgaca
    20581 tcgcccatat tctgagcgtt attccgaacg atcgcgccaa ggagatcctg gcgttgatgc
    20641 ggacggagga ctccactgaa atcgccgacc tgttgaagta ccccaagggc acggccggcg
    20701 gcatcatgac cacggagttt ttcgccctct tcgaggacgc cacggctcaa gaggcgatcc
    20761 gccgcctgca gcaggcgacc gacgccgaaa tggtgttcta catttatgtg accgataagg
    20821 aggatcgcct tgtcggggtc ttgtcacttc gccaattgct gaccgttccc cccgccacgc
    20881 cgctcaagaa catcatgacg cgagacctca tcagtgtcgc cgtcgacatg gatcaggagg
    20941 aagtctcgcg ccaggtggcg agttataacc tgctcgcgat tccggtcgtc gagaaggatg
    21001 ggaaattggt tggcatcatc accgtagatg acgtcgtgga cgtcatccgc gaggaagcca
    21061 cggaagacat gttgaagatg gccggcgccg ttgaggagga cgcgatgttt aagtcctcca
    21121 gcatggccgc cgcacgtgtc cgcttgccat ggctgtttac caatctggtg ggaagcctgc
    21181 tgtccgggat gctgctctgg atgttccggt acacgattca ggaggtcgtc gccattgtca
    21241 gtttcatccc ggtcatcgcg gcgatgggag gcaacgtcgg gctgcagtcc tctacgctca
    21301 tcattcgagg gctggcgacc ggcctcgtcg agctcaccga tgtctggaag attttctttc
    21361 gtgaagcgaa gatcgccttc ctgatgggga ttgcctgtag cctcatgctg acggtggtcg
    21421 gatggttgtg gcaccaggtg tttctcggga tggtggtagg ggtgtcgctc atcacggcct
    21481 ttctcgtctc caccagcatg gctacggtca tgccgatcgt cttgaagcga atgggtgtcg
    21541 atcctgcggt ggccgccggg ccctttgtga cgacggccaa tgatatcacc gggattgcga
    21601 tttaccttac cctggcaacg atcttcctcg agcagatccg ataaggatgc cgtgcgcacc
    21661 tcctcgttcc caagtcattc gcagggcagg gtgaagagat gccgctggta aagacggctg
    21721 cgattgtgct gcacagccgg aaatggggcg acgccgaccg gattgtcacg tgttacacgc
    21781 tgcggttggg gaaactgcgc ggcgtggcgc gaggggcgag gcggttgaag agccgcttgg
    21841 gtggcatgat cgagccgttt acgctttgtc agctggattt gtttgaaaag cccggggatt
    21901 ccttgtaccg catttcgcag gtcgcgttgg atgaaccgtt tgcgaaactt cgcagcgatc
    21961 tcggattgat gactgccgcc gggcgaatgg tcaatcttgt gagcgccgtc atggccgagg
    22021 gcgatgcgga accgcgtgtt tttgagatgc tggaatcggg cctgcgtacg ttgctggaga
    22081 gccgggacgc ggcctggacc accgtgttgt ttcagattcg tttgctgggc atcactggat
    22141 ttcgtccgca aactgaacag tgcgcgacct gcggacaagt acaccgaacg ccgcttccgc
    22201 aattttcgcc gctggccgga ggaatggtct gtgagcgttg tgcgagtcgc cagccgtttc
    22261 ggtgcacggc cttgtcgcgt ggcagcgtgg cctttctcca gcaggccctg cacatgcagc
    22321 cggcgctgct gaatcgattg caggcggcag ggcaggtgcg ggcggaagtc gaatcggtga
    22381 ttgaaagtta tgtcacggta gtggcaggtc ggcgtttgcc ggccgtggat tttctgacca
    22441 gcggcggacc tgcttaagtg cgacggtggt gttcgcaagg tctcctattc aaggcgtgag
    22501 ggagcgttga tcgctatggg tgaaacggtg ctcgagcccg tcgatatgga atgggtcgag
    22561 aagggtgtgc tgagcattac gtggagtgat ggccacaagg cccggtatcc cgtccgctac
    22621 ctccggcagc attgtccttg cgccgcctgt acggatgaat ggacgggaga acttcgcctt
    22681 aagcccgacg atgtgccgat ggttatcatg ttgcaggaca ttcagccggt ggggcggtat
    22741 gcctttcaat ttacctggag tgatgggcac gataccggaa tctactcgta ctcgtttctt
    22801 cgccggctct gccagtgtga tgtgtgccag ccggtgaaac cggtggaacc gcggtctcga
    22861 cggttgctgt gatgagatgc ggtcgctgtt tacagatgaa cagggaaagg cgggatcgga
    22921 tgaacgtctc taaactggca tccgtgggtt tgtggctgct gatcggactg atttctgtgg
    22981 cttgttcggg tctccctccg attgatcagc agaaacgatt ggttcaggcc ggagagctta
    23041 aaattcagca attgacgccg cgggcctttg ccgaaacctg gggcgatccg acatacaccc
    23101 atcagcagtt cacccatttt ttcggcatgc ccgatggtca actgattcct caggcgcgca
    23161 tggcgcttgg cgaatcaccc cagggatggg aaacaggatt ggctgctggg gatgccttct
    23221 tcatggccta tgctgatcgc ggatactacc tcgtgtttct tgaaggtgtg ctcgtctacc
    23281 atgaggccat gtcggccgag aaggtgcatg cggtcggcaa gacgtggaaa ttcgagtccc
    23341 agttcaagac gcggcttgaa tcctcccccg gtttgaaata ggcggtcgtg ttttctccat
    23401 gaatctgact ccggatcaaa cttccttgaa gatttgcatg gtggcatcag aggtcgtgcc
    23461 gttggcgaaa acgggtggcc tggctgatgt tgtcggcgcg ctggcagtcg aatttgccaa
    23521 gctgggacac gatgtctgcg tgatgttgcc ggcgtatcga caggtcgacg agctggggta
    23581 tgagatgacg gagggcgtcc gtttgtctgt tcccgccggg cctagcgtga tggatgtgcg
    23641 cgtgcaggaa gtgtttgcac cgccgctgca cgtacaggga cctgggcggc tgcgtgtctt
    23701 tgtcgttcgg catgatccgt atttttctcg ttctggtctg tatcaagagg ctggccgcga
    23761 ctatctcgac aacctggatc ggtttgtctg cttctgccgg accgtcatgg agatgtgcat
    23821 tcatctcagc gagcacgaag gatggcagac ggatctcctg caccttcatg attggcaagc
    23881 tgccctctgt gcggtctatc tacggacgct gtatcaagga cggccctcat tgcgccgtat
    23941 ccgtagcgtc ttgacgattc acaatcttgg atatcagggg gtgtttcccg cagcgcagtt
    24001 ttcttcgacg ggactgccgt tgcatctgtt cacgcctgca gccctcgagt tctatggaca
    24061 gatcaacgtg ttgaagggcg ggctggtctc cgcggatctc ctgaccacgg tcagccccac
    24121 ctacagcctg gaaattcaaa gctcggaata tggatgtggt ctggaagggg tgatcactga
    24181 gcgcaagagc gtgctgtctg ggatcgtgaa cggaatcgat acggagagtt ggaacccggc
    24241 cacggaccaa tatctggtgg ctccctatca tgcatccgac ctcgcaggga aggctcgctg
    24301 caagaaggcg cttcaacggg aattgggact gcgtccgctg aagtgcccgc tgcttggagt
    24361 gatcgccagg ctgaccagcc aaaaaggcat cgatctcgtc atcgatgtgc tgcccgagct
    24421 catggagctc gatgtgcaag ttgtgattct cggcactggc gagccgcact atgagcaaca
    24481 ggtgggtgaa ctggcccagc gttatccggg gaaaattgcg gtgcggaatg tgttcgatga
    24541 aggattggct caccggattg aagccggggc ggatatgttt gtcatgccat cccgctacga
    24601 gccttgcggt ctcagtcagc tgtatagttt acgttatggg acggttccta ttgtgagaaa
    24661 gaccggcggg ttggccgata cggtcgtggc ttataccccg cgagccttta aggaatcccg
    24721 ggcgaccggt ttcagtttta cggataccag ttctgcgtcg ctccttacct gcctgttact
    24781 cgcattgtcg gtgtatcgaa agaaagccga gtggcagcgt ctcgtgaaga ctggaatggc
    24841 acaggatctc tcatggagtc ggtcagcagc gatctatcta caattattca gagatgtgat
    24901 atctcgagca ggcgatcgat aaaactcgtt gcgttggggc gaggaagaaa tgaggaaggt
    24961 tatcgaagcg cagcttggag tgtcagttcc agctgagtga gccgctcccg tgcctgtgca
    25021 gtgtagtagg cataatccga ttccgcgtgg ccctgtaata cctctcgaaa ctgactcctc
    25081 gccacgtcta actccccggc cgacatggcc aggttccccg catgtaaact gcaggatgcg
    25141 gccatggcgt gaacatccac cgccaagcgc gaggacggct gactgaccat atttttcagg
    25201 ctggtaggca ggatggtatc gagcgtcgac cgcgtggttt gagctcggct gacgactcgt
    25261 aacttggtgg agtcctgcaa ggctacttgg ctgtcctccg ctgagaggca atgagtgtag
    25321 gtgctccata catccatgaa acggacattg tcgtaccgca ctgaggtgac ggcaggattg
    25381 gattggcagc ctacggctgt gaggcccgcg agtaagagga tgatttggcg taaggtgtga
    25441 tatcgcatgg tgccagtctt tgtcgatctg ccctcgactc ttgtcttgtc ctcggaatga
    25501 gcaagtcttg cgccacgtgt ggcacatgta agtcattgaa ttaattgtgg ccatttgtca
    25561 tcctctgaag cggtgctgtc tcaatttaag ttcactgtgg ggaatacgac acggatgatg
    25621 aggccacgcg gcacaggcca gatgaagggc tgttgagcag gccgaggctc acgaagtgag
    25681 gaagatcctg gagggtgcgg gaagtgtcgc tttccggccg atggctggtg ctgtcgatcg
    25741 cgtcctggac actgcggacg gtttgaacta accgaatgta tcccaattcg tcagtgaaga
    25801 tgcacagagg cgtcaaggca ggcctatggg gtatcgttgc ttgtctgtca agaatcggta
    25861 gcgccagggc tgcaggctcg gctactatct ggccatcgcg acggaaatgt gccgacctta
    25921 ctctgggccg acatatgcgt tctcgactca ttcatctctc agaaagaatg ggtgcgattc
    25981 gcgaatcatt gacgacgaat attcttgcga cttgatggtc tttcatatgg tacgaagaat
    26041 atcttcgtcg acgaacgaga cagaacttcc tcaggaaagg ttccttcgcc aatgaattcg
    26101 agacaaccgt taaccaagac ccgccagttt cgaaatttgc tcctctccga gcaactggag
    26161 tttatttgtg aagcacacaa tggactcagt gccaaaattg tagaggaggc ggggttcccg
    26221 ggtatctggg ccagcgggct ctctatctct gctcagtttg gcgtgcgtga taataacgaa
    26281 gcgagttgga ctcaggttct cgaggatctg gagttcatgt cggatgccac taacattccg
    26341 attcttctcg atggggatac ggggtacggc aattttaaca acatgcagcg cctcatccgt
    26401 aagctggaac agcgcaacat tgcggccgtg tgcatcgaag acaagctctt tcccaagacc
    26461 aacagctttt taaagggcga tgcgcagccg atggccgata tgcatgaatt ctgcggcaaa
    26521 atcaaagccg ggaaagatgc ccaaaccgat cccgatttct gcgtcatcgc acgagtggaa
    26581 gcctttatct gtggctgggg tttgacggag gcattgcgcc gcgctgaagc gtatcaccag
    26641 gccggggctg acggtattct catccatagt gccctttcgg ttcctgatga gatactggcc
    26701 tttaagcggg aatggggaaa tcggtgtccc gttgtcatcg ttcctaccaa gtattattcc
    26761 acgccaacgg atgtctttcg ccagcacggc ttctcgatgg tgatctgggc caaccacatg
    26821 cttcgtgcag cggtgtcggt catgcagaaa actgcacggg ccttgaagca gagtgaaaat
    26881 ctcctgtcca tcgaagacaa ggtggcccct gtttcagaaa tcttccgttt gcagaatgcc
    26941 gccgaactgc tggaggctga ggagcggtat ctccctcgtg gcgccgaggg cacgtcagcg
    27001 gtggtgttgg ctgcatcccg aggtgaagaa ctgggtgagc tgacggttga gcagccgaaa
    27061 accatggtca aaattcaggg cacaccgatc ttgtctcata tcgtggatgc gtacaatgcc
    27121 gtgggcatga aggatatttt ggtggtccgg gggtacaaaa aagaagcggt caaccttccc
    27181 aacctgacct atatcgacaa tgcggactat tcgcaaactg gcgagttagc ctcattatcg
    27241 ctggcgttgc aatccaggaa gggagttttt cagccgacta tcgtttcgta tggcgatgtg
    27301 ttgttcaaca agtatatccc gcaggcgctg tgccaagagc cggatgactg tgtgatattc
    27361 gtcgatagca actggcgcga ccaaacgacg tatgcgcgtt tgggaggctt tgtcgaatgc
    27421 accattccca attcacggaa ggccttcaat gcgaaagtct atctgaagca gttaggaaat
    27481 cggatcccga ctgatgccac ccacggagtg tggatgggat ttcttaaact ctcttcgtcc
    27541 gcagcctgtg atatcaacaa tcttctcgta gagatgctga gggagccgga gaatcgcaag
    27601 gccggtattc ctcacttgct ccaggaattg ttgcggcgcg aatatcctat ccgggtgctg
    27661 tataccgttg gccattggct cgatatcaat agcctcgatg atgtggtgca ggccggaaat
    27721 ttttgatggc tcgcattctg tccgctggtg atgttttgaa acgggtggca gccagtctgt
    27781 gaagttcatc gctgtatcgt ctcatcggga cgtcttaggt tggagaactt gtgcatgatt
    27841 gatcctcgga aatttgtcga atgtttacag tcaaacagtg tggagttttt cactggtgtg
    27901 cccgattcgc tgctcaagga attctgtgct tgcctagagc aggtgcctca agccggtcgg
    27961 cacgtcatca gtgccaatga gggcggcgct gtggcgctgg cgctgggcta tcacctcgcg
    28021 accggaaaag ttccattagt gtatctacag aattcggggc tggggaacat catcaatcct
    28081 ttgttgtccc tggctgatgc ggaagtgtat tcgatacccc tgttgctcgt gatcggctgg
    28141 cggggcgaga cgggtgtgca tgacgagcct caacacaaga agcaggggcg tgtcatgctt
    28201 gctatgctgg acgctatgga gattccgtac tccattttag gccctgaagt gaatgaggct
    28261 gaaggagcgt tgagtgacgc cctggcctat gtccagaagc ataatgcccc gttcgcactt
    28321 gtcatcaaga agggtacctt cggcgccttc accggcgata agaggaaaac ggagattttc
    28381 gaattgacgc gagaagctgc gattcagcag gtgctggaac acttggacga acgtgatgtt
    28441 gtcgtgtcga ccaccggcat ggcctcccgt gaagtctatg aatatagaat gaataaaggg
    28501 caggggcacg agcgggattt tctcaccgtc ggcggaatgg ggcatgcttc tcagatcgcg
    28561 ttaggtgttg cccttcaacg gcccagccgt tccgtctatt gtctggatgg ggatggtgct
    28621 gttctcatgc acatgggagc gcttcccatt actggaacgt tgaagccgga aaactttaag
    28681 catattgttt tcaacaatgg tgcgcatgat tcagtgggtg gacaaccaac ggtggggctc
    28741 gagatcgaaa ttcttgagat cgccaaggcc tgtggctatc agctggttgt tcaggcccag
    28801 acgccgcagg aggtacaagc cggtctccgg gagctaaagc ggtcgaaagt tccatgtcta
    28861 ttggagattc gaatacgccg aggagccaga aaggatctgg ggcgccctgc aaccacgcct
    28921 gtcgagaata agagcgcctt catggctttt gtccaaggcg gctaatcgac tggggaataa
    28981 ggggcgagct gaacctcgcg caaagtcgct gggcgtgctc cttgcgctgg agtgataggt
    29041 attttcctgc tggaccggcg aacagaggga tcttatgatt aagggcaggc gtgggtgagt
    29101 atcggaagtc gcgggattag ggaaatgatt gacgcattgg gcttacgatt taaagatctg
    29161 aacgagaact cctgtacgcg tcgtggagtt cgtttttcgc ataatgtgct tgatgtgttc
    29221 tttgaccgtc tgttcggtga ttttcagtgc gtttgctatt tctttatttg tccatccttt
    29281 cgctaggttt tcgaccaccg actgctcccg atttgtcagc tggaagcgtt ccttagcttg
    29341 gtcagtattg agttgttgcc gacgacccaa ctcttccaat gtgaccacta ggcgcgcata
    29401 ttgaacacca cctctatccg ggagaccaaa tcctcgcaag agcacgggtt gattcgggtt
    29461 gcccgcaatt tttttgacct cgaattgctc ccaatccttt gcctctgtgc gaacttggag
    29521 cgctttcagg atttcggtac agagctcagt aagagcggat ggaagcactc cttgagccgc
    29581 tttagctgga gtaccacctt tctctgccat gttaatgagc ttagataatt cagcggcttg
    29641 ccggttcata tgtaggagtt gcatggagga cgacagaacg acaattcctg cccccgtgcg
    29701 ttgatctgcc aagttgtccg cggtttcttg cgaggttata gcgatttgag ccacggcaat
    29761 actccgtatg atgctgaatg ttcctcgaca actggtcgat ttctgataac ctacagtgtg
    29821 tcggcgttac tagtaatgct acataatact ttcgaggtag tcaaatgata ccttcggggg
    29881 ggcggtccgt acatcattgg tattgtccag tgagaccatt gattctatac ttcaaaacgg
    29941 tggcggactt ctttagtatg agagccagca ttgatttgtc cgtatttgcc cattcctctc
    30001 ggttgattct ttagcataag gcacccatta tcctaaaaaa tcgatagctt ccaaggtttg
    30061 tgcaatttaa acaatgtgtt atgggatgaa ttggggtgtc ggattgaggg atgtgtgtga
    30121 ccgagacgat tgggaacatt cagggatttg atcgcaatcg gctgaacttg agaacggtct
    30181 cagagcgctg tgctgtaatg ggcaaccgga attctttcag gagaataagt catgaaagct
    30241 aaggtcgtat tgagcctaat ggtcctggct ggaaattgcc tcaacgttgc atttgccggc
    30301 ccgtttgatc aggcccccaa ggctactctg gtagccggag ctcctgcagc ggcacctcct
    30361 tcagggatgc gtcatggggg ggatggagca gaaaagtctt ccttgaccgt gacgccggat
    30421 tacattatgg gtcctgagga tgttctggaa attaccgtgt ggaaaaatgc ggacctttcg
    30481 aagcaagtcc aggtcagacc tgacggaaga atttctcttc cattgatcgg tgacgtttca
    30541 gccgttggcc gcaccgctgc acaactaacg gaagaaattt ctgcacgctt aaagtcttat
    30601 atggagaatc ccaccgtttc catcctggtt cgggaagtga acagctacca aatctatgtc
    30661 ctgggggaag tgaatgcgcc cgggaagtac ccactgaaaa gtaagaccac tctgttgcaa
    30721 gcgataacca tagctcatgg atttacccaa gtggccgccc gtaacaagat tgtggtcttt
    30781 agattcggaa aagacggcga ggggcttaac aaaattaagg ccagttatga cgatatcgtc
    30841 ttaagagatg gctcagatca aaacatcgaa ctcaagccgg gagatcagat cgttgtaccg
    30901 tctgaaacta tggttgtatt gccgtccaga taattgtcgt tagtcaaggt gctcagacga
    30961 tatttcgtat cgacgagcca aaggagttag gaaagtgaaa actttcggtt gcagatggtt
    31021 acgagggggg gtatgtattg tttttagtgt gcttctgact ctcccaggat gttggatagg
    31081 caacgagcaa atagacttta acgtcaccta tattccccca aatgagtttt tgttaggccc
    31141 tgaagacgtt cttgttgtaa atgtgtggag aaatcaggag ctttcccggg aagttattat
    31201 tcgacctgat gggaaaatct ccatgccgct ggtcggtgac gttcaagcgg caggcatgac
    31261 ctcaaatgtg ttggcgaagc gtattgctga tggattggct gagttcatgt ccaatcccac
    31321 agtttcagtc caggtcaaag aggttaatag ctattacgta tatgtattag gagaggtctc
    31381 gaagccaggt aaggttccgt taaagtcgta tgctacagtt ttgcaaggca tatcattggc
    31441 tggcggattt acgacgtttg cctcgagaaa taagatccat gtactgcgag tcgttccaaa
    31501 tggccaaggt caaccaaagc aggtccagat cccggttccc tatgaagaca ttctgcaagg
    31561 taaaaattcg gagggaaatt ttttcctcaa ggctggtgat gtcattgttg tgccgtgacc
    31621 agtttttgag gatggggaaa ttcgctttaa tatgtttgac actcgcgttc gtgtcatgct
    31681 ggttcgcgtc accatcttcg gctcagcaaa tacgtgttgt gccatcaatt tcggttcttg
    31741 agcaatacga cagcaacgtg ttcttcactc c
//
LOCUS       DAOQDS010000048        31059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_499_length_31059_cov_21.896497, whole genome shotgun sequence.
ACCESSION   DAOQDS010000048 DAOQDS010000000
VERSION     DAOQDS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 31059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31059
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_499_length_31059_cov_21.896497"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..850)
                     /gene="cmk"
                     /locus_tag="PK589_12655"
     CDS             complement(152..850)
                     /gene="cmk"
                     /locus_tag="PK589_12655"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNJ20504.1"
                     /translation="MGEIGVASRGKRGLIIAIDGPAGVGKSTVARLLALRLGYLYLDT
                     GALYRAIAWKVHNAGLSPDDHLAIAALLPKTTLQMACGPEQSHVLLNGRDITGELRTP
                     AVSALASMVSAIPAVREWLLPVQRQIGAEGCVVAEGRDIGTRVFPEADVKFFLEADAE
                     VRATRRHRELVAAGHSVQFDQTKRDLTGRDDRDRTRAVAPLIPASDAERLDTSSLPVE
                     EVVSHMMAVVAARL"
     gene            complement(863..2191)
                     /gene="aroA"
                     /locus_tag="PK589_12660"
     CDS             complement(863..2191)
                     /gene="aroA"
                     /locus_tag="PK589_12660"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNJ20505.1"
                     /translation="MASLTITPGRPLKGTITVPGDKSVTHRAIILTALAEGSSTVKEY
                     CRGEDCLNTMRAFQSLGVRIEETPESLTVYGKGMWGLTEPFGPIDCGNSGTGIRLMAG
                     LLAGQDFFTVLTGDESIRRRPMGRVVKPLRAMGATIAGRKGGELAPLAITGTRLKGLS
                     YVSPVASAQIKSSLLFAALYADGLTTISEPRLSRDHTERMFAYFGIPFERDGCTVRIE
                     GRPSVRWSGKPVLVPGDLSAAAFFLVGASIVPGSDVTVRNVGMNPTRTGLIDILREMG
                     AHIEVLNPREEAGEPVADLRVLSRPLHGVRIGPDQIPQTIDEFPILCVAAAVAEGETV
                     VTGAEELRVKESDRIATMAKELRAMGARIEERPDGMVIQGLGHNGANGALAGAICESH
                     GDHRVAMSVAIGGLTAAQATQIQNTACIETSFPNFDRKLLELLTDSGKRL"
     gene            complement(2198..3094)
                     /locus_tag="PK589_12665"
     CDS             complement(2198..3094)
                     /locus_tag="PK589_12665"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNJ20506.1"
                     /translation="MTTPHFKQAAIIGVGLIGGSLGMILRRQKLADSVVGIGRRVENL
                     KTAVEVGAIDRYVSDPREGVEQADFVLLATPVDTYERHLQEWAGCLKPDTIVSDVGSV
                     KGELVTRAEALMPSGVKFVGAHPIAGKEKTGVAAGSETLFSGARCILTPTTHTDPEAL
                     KVVRKIWELAGSIVLEMDPFLHDKILGAVSHLPHAAAFALMTALADVRDHGVPELDLA
                     GHSGGGLRDTTRIAASSPEMWRDIFLWNRDNVVSLIETYERHLGELKRLIAAGDAAGI
                     EKQLDKAKYEREQLIPRTPGKA"
     gene            complement(3150..4163)
                     /gene="aroF"
                     /locus_tag="PK589_12670"
     CDS             complement(3150..4163)
                     /gene="aroF"
                     /locus_tag="PK589_12670"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNJ20507.1"
                     /translation="MIIVLKPDASEREVDHITDRLRELGLKSHISAGQERTIIGVIGD
                     DRILHNQPLTALPGVESVLPILAPWKLVSREFKKENTIIDVNGVKIGDKKITIMAGPC
                     AVERLELTVGIAHEVKSAGATVLRGGAYKPRTSPYSFQGLGREGLDYLVEAKKQTGLP
                     VVSEILDTRDIELFLEKADIIQIGARNMQNFELLKEVGAYDKPVLLKRGLSATIKEFL
                     LSAEYVMSRGNRNVMLCERGIRTFETQYRNTLDLAAIPTLKDLSHLPVIVDPSHATGK
                     WDLVAPMSKAAIAAGADGLLIEVHSNPECALCDGEESIRPSKFKELMGDLRKIAAAVD
                     RTL"
     gene            complement(4182..5267)
                     /gene="hisC"
                     /locus_tag="PK589_12675"
     CDS             complement(4182..5267)
                     /gene="hisC"
                     /locus_tag="PK589_12675"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNJ20508.1"
                     /translation="MPLKVHPDIVSLVPYVPGKPIEELQREMGLPRVVKLASNENPIG
                     PSPKAMAVLAETVPTLHRYPDGGAFRLRGALAERWKVAPDQVILGNGSDEILGLLART
                     FLSPGDEAVMAEHTFVIYKMEVQAAHGVAVEVPQKNWHHDLPAMAAAITEKTRLLFVC
                     NPNNPTGTMATKAEVAALMERVPDHVVVVFDEAYYEYVRHSEFPESLAYVKAGRPVIV
                     LRTFSKIYGLAGLRIGYGITTPEITNYLNRIRPPFNANSMAQRAALAALDDDAHVAAS
                     RSLNHVEMDKVRAGLRWLGFEALPSETNFLYFDVGRNGRAVFDALLAKGIIVRHIEGR
                     MLRVTIGLPDENQLFLSALEDVTRASQ"
     gene            complement(5284..6360)
                     /gene="pheA"
                     /locus_tag="PK589_12680"
     CDS             complement(5284..6360)
                     /gene="pheA"
                     /locus_tag="PK589_12680"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNJ20509.1"
                     /translation="MSDDLQVHREEIDRVDDQILRLLNERSKSVIEIGRLKKLKDADA
                     NLHTQARESAIFDRLSKQNTGPFPTEAIRAVYREIMSASLSLEGPQKVAYLGPRATFT
                     HMACMQKFGASAQYIPVNSIKDVFSEVERGRAHFGVVPIENTTEGVVNHTLDMFVDSN
                     LLIYGEVLQEVAHHLMSKSGLAGDIKRIYSHPHAIAQCRNWLETNLPHVPVSEVASTA
                     RAAEMSVDDPSAAAIASELASQLYGLKVVTARIEDNINNFTRFLVLSQKAPERTGRDK
                     TSLMLSVKDKVGALYDLLRPFASHGLNMTKIESRPSRRKAWEYIFFVDIEGHIDEERV
                     KKAAEEVKSRCLFMKILGSYPAYS"
     gene            complement(6500..7537)
                     /gene="ruvB"
                     /locus_tag="PK589_12685"
     CDS             complement(6500..7537)
                     /gene="ruvB"
                     /locus_tag="PK589_12685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNJ20510.1"
                     /translation="MADRTVSNQLTDDERGLEAALRPQSLREYVGQPRMKESLEICIE
                     AAKRRGEALDHAIFYGPPGLGKTTIAHIIAREMGSAIRSTSGLVLSHAGDLAAILTNL
                     QERDVLFIDEIHRLPASVEEALYPAMEDYQLDLVVGQGASTRTVKLELPHFTLVGATT
                     RAGALTSPLRDRFGLVHRLEFYSSQELTSIVTRSAGLLNIPIDEAGAAEIARRARGTP
                     RIVNRLIKRIRDYAEIKAGGRITQRVAQDALVWLAVDEAGLDEMDRRVLLTVIEKFHG
                     GPVGVDSLAAAVQEDKGTLEDVYEPYLIQAGFLERTGRGRQATRLAYEHFKKQKDLLP
                     FSSETDSIATP"
     gene            complement(7567..8073)
                     /locus_tag="PK589_12690"
     CDS             complement(7567..8073)
                     /locus_tag="PK589_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20511.1"
                     /translation="MVMMELARKKQDRPGIAMALGVVAVLLCASMTDGVFAADGMDEP
                     KSIRKTCGSCPEGYATTGRTNAPEVCKDGDPVLVQCVPLGSNMLSVCGSCPEGYSEVG
                     RSNVPSRCGSVDGGLVSQCQSQQMGSSMPDPSQGGVRCPPDCGSTAAPGQGASPPPPK
                     FRPAPETR"
     gene            complement(8077..8685)
                     /gene="ruvA"
                     /locus_tag="PK589_12695"
     CDS             complement(8077..8685)
                     /gene="ruvA"
                     /locus_tag="PK589_12695"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNJ20512.1"
                     /translation="MIALLTGLVAFKAPSQVTLDVHGVGYEVLVPLSTYYSLPNQHEP
                     VTLSIHTHVREDAIQLFGFFTASEKEAFLLLTGISGIGPKLGLSVLSTLSVRDLYAAI
                     QAGDIEKLGTVPGIGKKSAARIALELKDKVAKLHPSGDLGVAGSGRSDNPLYEDALSA
                     LVNLGYRQPDVKEALKRVEQSGVLTQGLEQVIREALKDLAKG"
     gene            complement(8767..9522)
                     /locus_tag="PK589_12700"
     CDS             complement(8767..9522)
                     /locus_tag="PK589_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNJ20513.1"
                     /translation="MGGHSHWATIKRHKSAVDAKRGKVFTRIIRELTIAARSGGDPDG
                     NPRLRLAIAKAKEANMPGDTMKKAIQRGTGELPGVTYEEFTLEGYGPGGTAVLMEITS
                     DNRNRTVAEIRSLLTKNGGNMAEAGAVAWQFHKKGVLVVEKGKVEEDALLTIALEAGA
                     EDVKVSDKAFEVLTSPHDFEAVKKALSDAKIEYTLAELNFIPQNTIALEEKAAEQMLK
                     LMEILDEHDDVQKVHANFDISDEVMEKVAAATA"
     gene            complement(9666..10373)
                     /locus_tag="PK589_12705"
     CDS             complement(9666..10373)
                     /locus_tag="PK589_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20514.1"
                     /translation="MVLASTGSFSIAESDDHGHSSTENHGHHHESSPSASQWEGSREG
                     IAYSEFNHHLAGVFLLLIGLSELRQALGWPVLTWTRFLLPGALAVAGLFLLIWSDHDA
                     WPIGSMTFAETFFGKDPEVLQHKTYGVLALAVGTIEFLRRLGRSATAVWTAPLPIFAV
                     VGGFMLFSHSHGDHPAADKIALHHLMMGTMAIIAGSSKLISGWRQRQLATERSYWELV
                     WAILVLVIGVQLLFYSE"
     gene            complement(10520..11275)
                     /locus_tag="PK589_12710"
     CDS             complement(10520..11275)
                     /locus_tag="PK589_12710"
                     /inference="COORDINATES: protein motif:HMM:NF019968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HNJ20515.1"
                     /translation="MKFVCLNCETYMTFQKVEKPGEGSLGVFFGCPSCEAKFSMVTNP
                     GETQMVASLGVKLGGRTETAEPFEMTRGTLKDEAQAGAGQMAAYLNEKIQGGQPATAA
                     PKATAPAAGGEKTSGGCPFSAMVAEMGLTSGGKPQNGNGTSEFTWTADAKEKLERLPS
                     FVKPMVQSSVEAYARKHGYKSITLQVMDDSKNDSPNGIAWTKDAEQRMDNIPDFIRPM
                     ARREIERIAKERGLVEITAQVMDEAKEKFMKFM"
     gene            complement(11302..12228)
                     /locus_tag="PK589_12715"
     CDS             complement(11302..12228)
                     /locus_tag="PK589_12715"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNJ20516.1"
                     /translation="MTMAAGKDLKSILTKLQYSDDAKVVQQITAQMKQVQARMAGIKQ
                     KLVVMSGKGGVGKSMTTVNLALAFARQGAKVGLLDVDLNGPCVPRMMGLHGQSLTMTP
                     EGAQPPVGPLGIKVASMDFFLGDASPVRWKGPMDLSPVWLGLMEMNVIREFLADVVWG
                     ELDYLLADLPPGAAADKPPVIAGFIPDLAGAIVVTTPSEVASDVVQKSVTYARDMGIR
                     VLGIVENMSEYRCPSCGAENELFEGNTEAMCEVLDLPLLGRIPFDRTLAKTFDKGQPL
                     LDPDYPTIRKYQEIVGRIQSLLDYKKVLAEKL"
     gene            complement(12307..14154)
                     /locus_tag="PK589_12720"
     CDS             complement(12307..14154)
                     /locus_tag="PK589_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNJ20517.1"
                     /translation="MTQHDQTKPQESLNIVIVGHVDHGKSTLVGRLYADTGSLPEGKL
                     EKVQAICRQQGKEFEYAFLFDAFLEEQEQGITIDTARTFFIWNGRQYIIIDAPGHKEF
                     LKNMISGAARAEAALLLIDALEGVREQSKKHGYLLSLLGVTQFAVVVNKMDLVGYRQD
                     VFDGIEKEYREFLGQFRAVPQQMIPVSAKMGDNIATRSTHMNWYQGPTVLETLALFKK
                     EQVRSEQPLRFPLQDVYKFDARRILAGRITAGRLKVGDQLVFSPSNKTAVVQSIEGFN
                     VDPPPSEAQAGQSVGITLDEQIFVERGEIASHRDSVPLVSTAVRVNLFWMGRRPLEKG
                     RKYVLRLATREVACEVAVIHRIIDTADLAQLQESQSVARNQVAELTLRVKSPLAFDLS
                     SSFESTGRFVLVDEYDISGGGIITELVHDEQEGLREEARQREYAWLTGDVRPEDRALQ
                     YGHRAAIVLFTGSAQTGKTFLARRVEALLVADSRHAYLLEGENLLQGLDADLSAADPS
                     FASERVRRYGEVARLLIDTGLIVVSTSKTFGINYQRMAEMIRTLVQPAPVIAVHMSRA
                     GEEPPPNTDLHFAGPQDFDAAARQIIEELKKRGVLIQPSGTKSNIQYSI"
     gene            complement(14229..15026)
                     /gene="cysD"
                     /locus_tag="PK589_12725"
     CDS             complement(14229..15026)
                     /gene="cysD"
                     /locus_tag="PK589_12725"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNJ20518.1"
                     /translation="MKHLRQLEDQSVYILREAYKHFNHLAMLWSMGKDSTVLLWLARK
                     AFFGHVPFPLLHVDTSYKIPAMIEYRDRIAREWRLNLVVGQNKEALAAGMNHTLGRDV
                     CCTALKTNAMKNLVEEKGYTGIILGVRADEDSTRAKERYFSPRDKHGDWDFRDQPPEL
                     WDQFKTTFPPGTHIRIHPLLDWTEINIWEYIKHENIPFMDLYLDRGDGTRYRSLGCAP
                     CTMPIKSTAKTVDEIIVELRKSTVAERAGRAQDAGRGMEMLRKKGYM"
     gene            complement(15044..16111)
                     /locus_tag="PK589_12730"
     CDS             complement(15044..16111)
                     /locus_tag="PK589_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNJ20519.1"
                     /translation="MQHLLAKVAKGQKTSKDLTWEEAKQAMRLMIEGTATQAQIGAFL
                     TAMRFKSESVTELAAFTATARQYVPPVPVRSGLGVVDVPVYAGKRETFHAIVPAAIIA
                     AAAGAVVLLHGVDGPADRRGVSSVLKLLGIPTDQTAKLVGPELEKKGFAYLDLALYHP
                     PVSRFLEMRQELGVRNFFHPVARMLNPARAASQVIGLSHPPYFEKTIEALRMLSCPRA
                     LVIRGVEGDPELSIGNSTRLLELKDERITPFTFQPKDAGLAMATFREMAGFPAEQRER
                     EADLIKRLVANEIQGGQRDWVLLNAAMLLYAAGKGTSITGNLAAARNALESGRAKAKL
                     SELIAGPGSETAAGSKVGLSA"
     gene            complement(16154..16876)
                     /locus_tag="PK589_12735"
     CDS             complement(16154..16876)
                     /locus_tag="PK589_12735"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNJ20520.1"
                     /translation="MQDTQKSAERPSDAELKALSDSFESQQPWDLLAYALKTYQGRIV
                     LACSFGAEDVALVDMVHRIDPDAPLFYLDTDFLFPETFDVRDRIVARYGLKPAQVIQM
                     KSLLTPEQQAAQHGAALWASKPDQCCELRKIEPLTRILGQYSAWITGIRRDQAPTRAN
                     AGLIEWDKKFQLVKVNPLAAWSTEQVWTYIQLHEVPYNILHDRNYPSIGCTHCTAPVL
                     PGDDPRSGRWKNFGKTECGLHK"
     gene            complement(16883..19369)
                     /locus_tag="PK589_12740"
     CDS             complement(16883..19369)
                     /locus_tag="PK589_12740"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNJ20521.1"
                     /translation="MISSHTEALRPAVKTEPIPPHIVEEIETFEAEAQRVLAGDLSTD
                     IFKPFRLQYGIYGQRQPGVQMFRIKIPFGGLTANQLRRVAELADQFATGVGHVTTRQD
                     IQLHFVELKHVPEMMRQLAAVGLTTREACANTVRNVTACHLAGVCQGEVFDVTPYAKT
                     VAYHLLRNPLNQSLPRKFKIALSGCRQDCALTPIHDIGLLAAKRADGTIGFRMVAGGG
                     LGSTPRMAQVLREFTPMDELLPTIEAVIKVFDTLGNRKNRNKARMKFVIEKLGFDEFK
                     RRWEAAYAAMGYAVPTHEPIKLLEYADTPPLLMPTKAPNSTNGNGNGNGNGAASRNGA
                     ESAFEAWKRTNVVPQRQAGFAAAAIKLPMGDLTGEQMWVVADLAERYSNGNIRTTINQ
                     NVIIRWIQEDRLSAFHEELVAHSLGDPGAELVEDIIACPGTDTCGLGITSSKGMARAL
                     AEVFPAGQVPEDLRDVSVKISGCHNSCAQHHIATIGLHGVGKRLGEHTAPHYELHLGG
                     HVDGTPKIGQLAVKLPAKSVPAAVRHLVDVYRRDRKPGESLQTFIGRVGKNVLKDELI
                     PYTIVPAYDQDPTYYYDWEGEAEFVLEDLGPGECAGGALEMIDDRMLEADQELYQAKL
                     LVEKHQYALSVNKSYRAVLAAAKGLLVTEGIDPATDAETFQEFDVRLASKGIVPASYT
                     NLGAQVGDLGSKDATAETANDKMAFAKRFLSVCRAATEQMGKDLKLAQVKEEVVPTPA
                     PKVEPASPVATAPVYDLRGVACPMNYVKTKLKLEMMDNGEQLEVWLDAGEPIRNVPMS
                     LRNDGHKILEEGPLEPEAKHFKILVEKVEG"
     gene            complement(19418..19870)
                     /locus_tag="PK589_12745"
     CDS             complement(19418..19870)
                     /locus_tag="PK589_12745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNJ20522.1"
                     /translation="MKVSLRATYGIIAAVDLALHHAEHPVCAKSIAKRQAIPARFLEQ
                     VLHAMKKAGVVTSQRGAQGGYVLSRKPSELSVADILDALEGPLLSTNGDAAPKQTSSR
                     ASKQEALLAHIWDRVKRAELSVLSEVTVEELAKRQRALDEQHTLMYHI"
     gene            19885..20232
                     /locus_tag="PK589_12750"
     CDS             19885..20232
                     /locus_tag="PK589_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20523.1"
                     /translation="MGIHECLYIEPRFPVKPAGAGLVADIADALAAITLADNCSPSLG
                     RDEFAASKVSNLHCELMPPTISSTSAHFLHFDRSGCINTGADFLIDIIWGLSNNPALF
                     FLGASHDAAFINI"
     gene            20207..20791
                     /locus_tag="PK589_12755"
     CDS             20207..20791
                     /locus_tag="PK589_12755"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ20524.1"
                     /translation="MTLRSSTFNILLLSTDSEIQSHYKDLFGEQAITVGREGPSLQKD
                     LAKRDYDAVIIESKLAAAADLSKLLTGIDPSRTLLLVGSRTVLKRTAAVLQASKASKA
                     EAPPSNGKGQALCLDSYLEHKVGDFVKGMRNGSGKNLHPMLIAAVERPLILLTLRETK
                     GNQIQAAELLGLNRNTLRKKILDLDIPVKRARAS"
     gene            20760..21155
                     /locus_tag="PK589_12760"
     CDS             20760..21155
                     /locus_tag="PK589_12760"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNJ20525.1"
                     /translation="MSPSNAPGPASVPTTEPHLTRRHKYAHASHPRVANHQRREAMTK
                     EELIAQMANSAGITKVAATVALEAFTGAVTTSLKKGKRVTLVNFGTFTISKRKARMGR
                     NPRTGEALKIPAARIPKFSAGKELKAAVK"
     gene            21287..21361
                     /locus_tag="PK589_12765"
     tRNA            21287..21361
                     /locus_tag="PK589_12765"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21319..21321,aa:Val,seq:gac)
     gene            21449..23404
                     /gene="thrS"
                     /locus_tag="PK589_12770"
     CDS             21449..23404
                     /gene="thrS"
                     /locus_tag="PK589_12770"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNJ20526.1"
                     /translation="MASQLIHITLPDGTRKQVPTGCTVREALTPQGGRLDGKILAAKV
                     NGVPMDLFHALDQDASVEPLTFESAEGREVYRHSSTHIMAQAVKEVFPSAQLTIGPAL
                     DDGFYYDFAFDRPFTPEDLEKIEARAIEITKRGLTVSRSELSKEDAIKFFQDRGEAYK
                     VELINSFDDGSPISLYRQGEFVDLCRGPHLPTTGYVGAFKLLSTGGAYWRGDERNPML
                     QRVYGTSFPTKKELDAHLAKLEEIKRRDHRKLGKELDLITIQDDIGPGLVLWHPKGSL
                     IRLLIENFWREQHIKDGYDLVYSPHVARLDLWKTSGHVDYYRENMFASMKLEGSEYQL
                     KPMNCPFHIMIYKSHLRSYRDLPIRYGELGTVYRYERTGVLHGLLRVRGFTQDDAHLF
                     CRPDQIEAEVSRVLDFTFFVLGTFGFTEFEIYLSTRPEKSVGSDENWTIATNALEAAL
                     KSRNVAYQVDPGEGVFYGPKIDIKIKDVLGRSWQCSTVQVDFNNPERFKLAYTGEDGK
                     HHQPIMIHRALMGSIERFFGILIEHYAGAFPTWLAPVQAVVLTITDHQQEFAAKIVST
                     LKSHGYRVEADLRNEKIGFKIREAEKNKIPYMLVVGDKEVQSGMVAVRGRSKADHGTM
                     PIEQFLELIRTDTNQSLRDTATHSQTR"
     gene            23408..23914
                     /gene="infC"
                     /locus_tag="PK589_12775"
     CDS             23408..23914
                     /gene="infC"
                     /locus_tag="PK589_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNJ20527.1"
                     /translation="MVPKLRVNREIRIREVRVIGPDGEQLGILPTVEAFNKAQEGGYD
                     LVEVAPTSQPPVCRIMDYGKYKFELSKKDHQSRRHQKSTQVKEIKLRPRTDKHDLEIK
                     IRQIKEFLADGNKTKVTLTYRGREMANQEMGRTMMTSVIQQCMEAGTVEFAPRMEGRS
                     LIMILAPK"
     gene            23948..24145
                     /gene="rpmI"
                     /locus_tag="PK589_12780"
     CDS             23948..24145
                     /gene="rpmI"
                     /locus_tag="PK589_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNJ20528.1"
                     /translation="MKQKMKTHSGAKKRFRRTGTGKLVRRKAGGRHLLTGKPRDRKRN
                     LKGATEVSSASTPALNKLLPQ"
     gene            24189..24557
                     /gene="rplT"
                     /locus_tag="PK589_12785"
     CDS             24189..24557
                     /gene="rplT"
                     /locus_tag="PK589_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNJ20529.1"
                     /translation="MPRTKGGPKTRQRRKKRLKLAKGQYGAKSRLFRTATESVDKGQA
                     YAYSGRKQRKRDFRQLWIARISAATRLHGIAYSRFMNALKKANILLDRKVLSDMAIRD
                     MAGFEKLVGVAKQQLATAAS"
     gene            complement(24613..>25077)
                     /locus_tag="PK589_12790"
     CDS             complement(24613..>25077)
                     /locus_tag="PK589_12790"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20530.1"
                     /translation="EWANVVEVDNVMSLSYSCLRQWITPSLLQVETNSSRAFYPHRMF
                     EVGEVAVPDARADVGSRTITMLGAVIAHAGAHFSEIHSCLDLLLYYLDRPFRLEPVPH
                     PSFLEGRAGRIMLDGRAIGLLGELHPEVLEQWQIGVPAVALELEIDRLLDEA"
     assembly_gap    25078..25177
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25178..26151)
                     /locus_tag="PK589_12795"
     CDS             complement(<25178..26151)
                     /locus_tag="PK589_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNJ20531.1"
                     /translation="MPTISLQRDDLEALIAGHGETASRISIDQLEQWLMLVKGELKGH
                     NIETGELRIELQDSNRPDLWCCEGIARQIRTKQQGTASSYPFFKKQKRAAGKLVVAPG
                     LEQVRPYVAACTAIGYRVTASGLAQLIQTQEKLADIFGRKRRTVSIGLYRLAPIVFPV
                     SYDLVKPDEVRFTPLGMETVMTLREILMVHPKGVEYGGIVSGHDRLPILRDAKGQVLS
                     FPPIINSREIGEVQVGDDHLFLEVTGTDLHMVALTLNIFAANLADRGAVIAPVEIQSP
                     VKTAFGKRWHTPIDFGAPRTIPLKAIETALGEALGSKEVTTALKSYGYT"
     gene            complement(26164..27708)
                     /locus_tag="PK589_12800"
     CDS             complement(26164..27708)
                     /locus_tag="PK589_12800"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNJ20532.1"
                     /translation="MDNLHPLESKVLLALAPQPGSPSTLDQLADSTGLEPSQLSMAVE
                     WLLAKSLIAVHAETVAYIASLTPIGEVFFEKYAPIERVLSAAREAGQTGKRLTIQDIQ
                     AKEVLEPSDVSKAVGTLKKEGAILIVQGGCIESTGRNSSTAEAMRALLQELRSGQREL
                     QTFAEPLRAVLQQHAVKRGNAREPFRVDERVTRSFLLTPSGQEVADQLSRDGVAEEVS
                     QLTPELLKEGAWRTKQFRKYTISLRAPRIAAGKRHPYREFLDLVKLKLVSMGFQEMRG
                     TLVETEFWNMDALFMPQFHPARDIHDVYFVKNPTHARNIDEPYLTNVTQVHENGAGAG
                     STGWGYQFDAERAKRLVLRSQGTAVSARRLAANPSVPGKYFSIARCFRYDQVDATHAT
                     DFFQVEGIVLGTDINFRTLLGLLNLFAREVAQAKEVKFLPAYFPFTEPSVEMHVRHPK
                     LGWMELGGAGLFRPEVTTPLGVSVPVIAWGLGLDRMAMVALGIHDIRDLFSADLEFIR
                     TMRGSF"
     gene            complement(27736..28440)
                     /gene="rpe"
                     /locus_tag="PK589_12805"
     CDS             complement(27736..28440)
                     /gene="rpe"
                     /locus_tag="PK589_12805"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNJ20533.1"
                     /translation="MAGRTVCIAPSILSADFARLAEEVAKVEEAGADWLHVDVMDGHF
                     VPNLTIGPPVVEALRKVTSLPLDVHLMMTNPDAFIGEFAEAGADYLTVHVETCPHLHR
                     TVQLIKERGVKAGVTLNPATPAITLSEIVGDADLILIMSVNPGFGGQKFIPSSLQKVA
                     AVRAMIDRTHSHALLEVDGGVKPDNADSILAAGADVLVAGSAVFSDHDYAAAITALRA
                     GRVSPPRSSRTMAGQR"
     gene            complement(28440..29879)
                     /gene="rsmB"
                     /locus_tag="PK589_12810"
     CDS             complement(28440..29879)
                     /gene="rsmB"
                     /locus_tag="PK589_12810"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNJ20534.1"
                     /translation="MGTDPQPASPGHAVSPGRHAAVKALLAIEEAGTLSDDLFDQISA
                     KAGLDLRDRAFMVELVRGVLRYRATLDWRLGLLSDRRIAKLPTLVQIMLRLGAYQVLY
                     LDRVPASAAVNESVQMMKQLRRRLGRDWSGFVNAVLRAMLRASPPDWPDLARNPVEAL
                     AVQYSCPTWLVERWCRQWGAERAATLCRASVEVPPLTLRVNRLRTTRAALLADLGAAH
                     VEAEPTSISEVGIQLGRTSLVADLPGYPAGHFYVEDEAGQLVPLLLDVQPEHRVLDAC
                     AAPGGKATHIAALMANRGEIIAVDRVASRLDRVMDNCRRLGVTIVTPVVADMRELKAE
                     EAGAREPAISPALPEVQSRPFDRILLDAPCSGLGVLRRHPEAKWYKTPDSIAQHRQVQ
                     LDLLATTTRLLRPGGVLVYSTCSIEPEETESVIDEFCQSHREFQRDSIAPWLPPAGLP
                     FLTPRGDLSTMANRNNMDAFFAARLRRVG"
     gene            complement(29938..30888)
                     /gene="fmt"
                     /locus_tag="PK589_12815"
     CDS             complement(29938..30888)
                     /gene="fmt"
                     /locus_tag="PK589_12815"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNJ20535.1"
                     /translation="MRIVFMGTPDFAVPSLEALLKSDDQVVGVVTQPDRPKGRGQEVI
                     PSPVKVVCQREGIPVLQPLKMKDPAFLDALREWQPDVIAVTAFGRILPPVILTLPPRG
                     CINVHGSLLPKYRGAGPLQWAIIRGERETGITTMFMAEGMDTGDMLLREAVEIRPDDT
                     AGTLAPRLADVGGRLLVETLRRLKAGTLTPERQDDSQATMAPLLKKEDGLIDWTLSAQ
                     EIANRVRGLSPWPGAYSYVNGERWTLCRVAVGDASQQAAPGTVTKVTKDRIDVATGAG
                     AIQILDIQPSNSRRMTVAQYLAGHRVAEGISLHAVPPPQA"
     gene            30976..31052
                     /locus_tag="PK589_12820"
     tRNA            30976..31052
                     /locus_tag="PK589_12820"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31010..31012,aa:Met,seq:cat)
BASE COUNT         6639 a         9438 c         8418 g         6464 t
ORIGIN      
        1 cagcccagca aaggaatatc caagtaactg gcgtggttcg aggcgatcaa caccccgccc
       61 ttccgcggca cctgaccgat ggtgcgatac cggaaacaga gccagccgat gatgcgagag
      121 aggacccaca agaggccata cagggcggag ctcacaatcg cgccgcaact acagccatca
      181 tgtgcgagac gacctcctcc accggcaggc tcgacgtatc gagccgctcc gcatcggatg
      241 cgggaatcaa gggcgccacc gcgcgggtac ggtcacgatc gtcgcgcccg gtcaggtcgc
      301 gcttggtctg atcaaattgc actgagtgcc ccgccgccac cagttcgcga tgccgccgcg
      361 tagctcgaac ttctgcatcg gcttcgagga aaaattttac gtcggcttcc ggaaataccc
      421 tggttccgat gtcacgccct tccgccacca cacacccttc agcgccgatc tgccgttgca
      481 cgggcaacag ccattcgcga acggcgggaa tggctgagac cattgacgcg agcgcgctca
      541 ctgccggagt tcgcaactca cctgtaatat cgcgcccgtt caggagcaca tgggattgtt
      601 cagggccaca ggccatctgc agggttgtct tgggaagcaa cgcagcaatg gcaaggtgat
      661 cgtcaggact gagtcccgca ttgtggactt tccacgcaat ggcccgatac aaggcaccgg
      721 tatccagata gagatatccg agccgcaagg ccaacagtcg cgcaaccgtg ctcttcccca
      781 cgccggccgg gccatcgatg gcgatgatca agccacgctt gccgcggctg gcaaccccaa
      841 tttcacccac gcgatcctcg cactatagac gtttcccaga atcagtcaac agttccaaga
      901 gcttacgatc aaaattcgga aaggatgttt cgatacaggc cgtattctga atctgcgttg
      961 cctgggccgc agtcaacccg ccgatcgcta cagacatcgc cacacgatga tcgccatgac
     1021 tttcacagat ggcgccggcc agcgcaccat tggcgccgtt gtgtccgagc ccctgaatga
     1081 ccataccgtc tggccgctct tcgatacggg ctcccatggc ccgcaactct ttcgccatgg
     1141 tcgcaatccg gtcgctttcc ttcacgcgca gctcttcagc gccggtgacc acggtttcac
     1201 cttccgccac ggctgccgcc acacagagga tgggaaattc atcaatggtc tgaggaatct
     1261 gatcgggtcc gatgcgtacg ccgtgaagcg gtctggagag cacccgcaaa tccgctaccg
     1321 gctcccctgc ctcctctcgc ggattcagca cctcgatatg cgcgcccatc tcacgcagga
     1381 tatcgatcag tccggttcgc gtcggattca tgcccacgtt gcgaaccgtg acatcggaac
     1441 ctggaacaat ggaggccccg accagaaaaa atgccgcggc cgacaggtcg ccagggacca
     1501 gcaccggctt accgctccaa cggaccgaag gacgcccttc gatccggacc gtgcatccgt
     1561 cccgctcgaa cggaatgccg aaatacgcga acatacgttc ggtgtgatct cgcgagagcc
     1621 ggggctcgga aatggttgtg aggccgtccg catagagggc cgcgaaaagc agtgacgatt
     1681 tgatctgagc gctcgcaacc ggcgaaacat aggacagacc cttgagcctg gtccccgtga
     1741 tcgccaaggg cgcaagctcc ccgcccttcc gcccggcaat agtcgcaccc atcgcccgca
     1801 acggcttgac cacccgcccc atgggacggc gcctgattga ttcatctccc gtgaggaccg
     1861 taaaaaaatc ctgccccgcc agcaatccgg ccatcagccg aataccggtt ccggaattcc
     1921 cgcaatcgat gggaccgaat ggttccgtca gtccccacat gccttttccg tagaccgtca
     1981 gcgattccgg cgtctcctcg atccgtaccc ccagcgactg gaaggcacgc atagtgttca
     2041 gacagtcttc accacgacag tactccttca cggtgctcga tccttcggcc aaggccgtga
     2101 ggatgatggc ccgatgcgtg acggacttat cgcccggcac cgtgatggtg cccttcaacg
     2161 gacgacctgg agtaatcgtt aacgaagcca tcgcagctta ggccttcccc ggtgtccggg
     2221 ggatgagttg ttcacgttcg tacttggcct tgtccaattg cttttcaatt ccagcggcat
     2281 cgccggcggc aatcagccgt ttgagctcac cgagatggcg ttcataggtc tcgatcaacg
     2341 acacgacgtt gtcccgattc cagaggaaaa tatctctcca catttccggc gaactggcgg
     2401 cgatccgggt ggtgtcacgc aagccgccgc cggaatgacc agccaggtcc agttccggca
     2461 cgccatggtc gcggacatcg gccaaggccg tcatcaacgc aaaggccgcc gcatgcggaa
     2521 ggtggctgac ggcaccgaga attttatcgt ggagaaaggg atccatctcc agcacaatcg
     2581 aacccgcgag ttcccagatt ttacgaacaa ccttcagggc ctcgggatct gtgtgggtgg
     2641 ttggcgtcag aatgcagcgt gcaccggaaa agagcgtctc ggagccggct gccaccccgg
     2701 tcttttcttt gccggcaata ggatgtgcgc cgacaaattt aacccctgac ggcatcaggg
     2761 cctcggccct cgtgaccaat tcccccttca cactgccgac atcgctcacg atggtatcgg
     2821 gcttgagaca gccggcccat tcctgcaagt gccgctcata agtatccacc ggtgtggcca
     2881 gcaggacaaa atctgcctgt tcaacgcctt cgcgcggatc agacacgtat cgatcgatgg
     2941 cgccgacctc aaccgccgtt ttcagattct ccacgcggcg accgatgccg acaactgaat
     3001 cggccagctt ctgacggcgc aggatcatcc ccagggagcc gccgatgagt ccgactccga
     3061 taatggccgc ttgtttgaaa tgtggtgtcg tcatgaggta attggctgat ccttgggaca
     3121 ttccctggtc aacgtcgctc cgcgatccct tacaatgtgc gatcgacggc agcagcgatc
     3181 ttgcgcaagt ctcccatcag ttctttaaat ttcgacggcc ggatcgattc ctctccgtca
     3241 cacagcgcgc attcaggatt ggaatggact tcgatcaata gcccatcggc tccggcagcg
     3301 atcgcggctt tggacatcgg cgctaccaaa tcccatttgc cggtggcatg gctgggatca
     3361 acgatgaccg ggagatggga aagatctttg agcgtcggga tggccgcaag gtccagcgtg
     3421 ttccgatatt gcgtttcaaa ggtgcggatg ccccgctcgc ataacatgac attccgattg
     3481 ccgcgggaca tgacgtactc cgcggaaagc agaaactcct tgatggtggc cgagagcccg
     3541 cgcttcaaca gaaccggctt atcgtacgcg cccacttcct tgagcaactc gaagttctgc
     3601 atgttgcggg cgccgatttg aatgatgtcg gctttttcca gaaagagctc gatatcgcga
     3661 gtgtcgagga tctcgctgac gaccggcaga ccggtctgct tcttcgcctc caccaaataa
     3721 tccaacccct cgcgtcccaa tccctggaag gaatagggtg aggtacgcgg cttatacgcc
     3781 ccgcctcgca acaccgttgc gccggcggat ttgacttcgt gcgcaatacc cacggttaat
     3841 tccaagcgct cgaccgcgca gggaccggcc atgatcgtaa ttttcttatc cccgatcttg
     3901 accccgttca catcgatgat ggtgttttct ttcttaaact cccggctgac gagtttccat
     3961 ggagccagaa tcggcaacac gctttccaca ccgggcaagg cggtcaacgg ctgattgtgc
     4021 aagatccggt cgtctccgat caccccgata atcgtgcgtt cctgtcctgc cgagatgtgg
     4081 gacttcagcc ccagttcccg aagccggtcg gtgatgtgat cgacttcccg ctccgaagca
     4141 tcaggcttca agacaataat catggtctcg tctcgctttc cttattgcga cgcgcgtgtg
     4201 acgtcttcca gcgcgctcag gaacaattga ttttcatccg ggaggccaat ggtcaccctc
     4261 aacatgcggc cctcgatgtg acgcacaatg atgcccttgg ccaacaacgc gtcaaagact
     4321 gcgcggccgt ttctcccgac atcgaaatag agaaaattcg tttcactggg caaggcctcg
     4381 aaccccagcc agcgcagacc ggcacgcacc ttgtccattt ccacgtgatt gagtgagcgg
     4441 cttgccgcca catgagcgtc gtcgtccagc gccgccaagg cagcgcgctg cgccatactg
     4501 ttggcattga agggtggacg gattcgattc aggtaattgg tgatttcagg ggtggtgatg
     4561 ccatacccga tgcgtaaacc ggctaatcca taaatcttgg aaaaggtccg caagacgatg
     4621 acgggtcgtc cggccttgac gtaggcgagt gattccggaa attccgaatg ccgcacgtat
     4681 tcgtaatagg cctcatcgaa cacgactacg acatggtcgg gcacccgctc catgagggca
     4741 gctacttccg ccttggtcgc catggtgccg gtcggattgt tgggattgca gacaaacagc
     4801 aatcgcgtct tctcggtaat ggcagcggcc atggccggca aatcgtgatg ccagtttttc
     4861 tgcggcacct ccaccgccac accgtgggcc gcctggacct ccattttata aatcacgaag
     4921 gtatgctcgg ccatcacggc ctcgtctccc ggcgacagga aggttcgtgc gaggaggccg
     4981 agaatttcgt cggagccgtt tccgagaatg acctggtcgg gtgccacttt ccagcgctcg
     5041 gctaaggccc cacgcaggcg gaacgctccg ccgtctggat accggtgcag ggtcggaacc
     5101 gtctcggcca gcactgccat ggccttaggc gacgggccga tcggattttc gtttgacgcc
     5161 agcttgacca cgcgcggcaa tcccatctct cgctgtagtt cttcgatggg ttttcctggc
     5221 acgtacggca cgagggagac gatgtcaggg tgcaccttca atggcatggt gtaactcggc
     5281 cctctaggaa taggcaggat acgacccgag aatcttcatg aacaggcagc ggctcttgac
     5341 ctcttctgcc gcttttttga cccgttcctc gtcgatatgc ccttcgatat ccacaaagaa
     5401 aatgtattcc caggctttgc gacgcgaggg acgcgattcg atcttagtca tgttcaggcc
     5461 atgggaggca aagggtcgca ataaatcgta cagggcgccg actttgtcct tgaccgataa
     5521 catcagcgat gttttgtccc ggcccgttcg ctccggcgcc ttttgtgaca acaccaaaaa
     5581 ccgcgtgaag ttattgatat tgtcttcgat cctcgccgtg acgaccttta agccataaag
     5641 ttgggatgcc aattccgacg caatcgcggc agccgaagga tcatcaaccg acatttctgc
     5701 ggcccgcgcg gtactggcca cttccgacac cggcacgtgc ggcaagttcg tctccagcca
     5761 atttcggcat tgcgcgatgg catgggggtg cgaatagata cgtttgatat ctcccgccag
     5821 tccgcttttt gacatcagat gatgcgcgac ttcctgcagg acttcgccgt agatcagcag
     5881 gttggaatcg acgaacatgt cgagggtgtg attcaccacg ccttccgtcg tgttctcgat
     5941 cggcacgacg ccgaaatggg cgcgtccgcg ctccacttcg ctgaagacgt ccttaatact
     6001 gttgaccgga atatactgcg cggaggcgcc gaacttttgc atgcaggcca tatgggtaaa
     6061 cgtggccctc ggcccaaggt aggcaacctt ttgaggcccc tcgagggata acgaggccga
     6121 catgatttcc cggtacactg cacgaatggc ctcggtggga aacgggccgg tattttgctt
     6181 gctgagccga tcgaaaatcg ccgattcgcg tgcctgcgtg tgcaaattcg cgtcggcatc
     6241 tttgagcttt ttcagccggc cgatctcgat gaccgatttc gaacgctcat tcaagagacg
     6301 caaaatctga tcgtcgaccc ggtcgatttc ttcgcgatgg acttgtaagt catctgacat
     6361 gacgggggga ttcccttctc gcaacacgcg cctttcacaa agacgggcta cgaaacgagt
     6421 ccaggccaaa ggggcgccgt tggacaagtg ggtatcctac aggagccact gcgggcattg
     6481 caaggatagg tctggggatt caaggagttg cgatcgaatc ggtctcactg gaaaacggca
     6541 acaggtcttt ctgtttcttg aaatgttcgt aggccaacct cgtggcctgt cggccgcgac
     6601 ccgtgcgctc cagaaagccc gcctggatca aatagggttc gtagacatct tccagcgtac
     6661 ccttgtcctc ttgaaccgcc gcagccagag aatcaacccc gacgggtcca ccgtgaaact
     6721 tctcaatcac ggtcaacagc acgcggcgat ccatttcatc cagccccgcc tcatcaaccg
     6781 ctaaccatac cagagcatcc tgggctactc gctgagtgat gcgacccccc gctttgatct
     6841 ccgcatagtc ccggatgcgt ttgatcagtc ggttgacaat gcgtggcgtt ccacgagccc
     6901 gcctcgcgat ctcagcagcc cctgcctcgt cgatgggaat attaagcaag ccggctgatc
     6961 gcgtgacgat tgaggtcagc tcctgggacg aataaaattc cagccgatgc accaacccga
     7021 accgatcccg cagaggggag gttaacgctc cagccctggt tgtggctccg accagggtaa
     7081 agtgcggcaa ttccagcttg acggtcctcg tcgaggcgcc ttgccccacg accagatcaa
     7141 gctggtagtc ctccatcgcc ggatagagcg cttcctccac cgatgccggc aaccggtgaa
     7201 tttcatcgat gaagaggaca tcccgttcct gcaaattggt caaaatggcg gcaagatcac
     7261 ccgcgtgact taagaccagt ccggacgtcg agcggatcgc cgatcccatt tcccgggcaa
     7321 taatgtgcgc gatagtcgtc ttgcccagtc ccggaggacc gtagaaaatc gcatggtcca
     7381 acgcctcgcc gcgacgcttg gccgcttcga tgcaaatctc caaggattcc ttcatccgcg
     7441 gttgcccgac gtactcccgc aaactctgcg ggcgcagtgc cgcctcgagt ccgcgttcgt
     7501 cgtcggtcag ttgattgctt acggtgcgat cggccatggc ataatctcga aagaagtggg
     7561 gcgcaactat ctagtctcgg gtgcgggccg gaatttcggc ggcggcgggg acgcgccctg
     7621 tcccggagca gccgtgcttc cgcaatcagg cgggcaccgg acgcctcctt ggctgggatc
     7681 gggcatactt gaccccatct gctgcgattg acattgtgac accagcccgc catcgacgga
     7741 accgcaacgc gacggcacat tcgagcggcc gacctcacta tagccttcag ggcaagaacc
     7801 acaaaccgaa agcatattgc tgcctaaggg cacacattgc accaagaccg gatcgccgtc
     7861 tttgcagact tccggcgcat tcgtcctccc ggtggtggcg tatccttccg ggcacgagcc
     7921 gcaggtttta cggatactct tgggctcgtc catgccgtcg gcggcaaaga ccccgtccgt
     7981 catggacgcg cataataaga cggccaccac gcccaaggcc atcgcgatac cgggacgatc
     8041 ctgtttcttt cgtgcgagct ccatcattac catgtgtcac cccttagcca ggtctttgag
     8101 ggcctcccga atcacctgct ccagtccctg agtcagcaca cccgattgct ccactcgctt
     8161 caacgcctct ttcacgtccg gctgccgata accgagattc accaacgccg atagggcatc
     8221 ctcgtagagc ggattatcgg atcgacctga gccagccaca cccaggtctc cggatggatg
     8281 caacttggcc accttatcct tcagctccaa ggcgattctt gccgccgact ttttgccgat
     8341 tcccggtaca gtccccagct tttcaatatc acctgcctga atggctgcgt acaggtcacg
     8401 cacagagagc gtggataata cgctaagccc cagcttcggt ccgataccgg aaatgccggt
     8461 caacaagaga aaggcttcct tctccgaggc agtgaaaaac ccgaacaact ggatggcatc
     8521 ttcccgcaca tgggtatgaa tgctgagcgt gacaggctca tgctgattgg gcagtgaata
     8581 ataggtactg aggggaacta agacctcgta ccccacgcca tggacatcga gcgtgacttg
     8641 cgagggcgcc ttgaaggcca caaggcctgt cagtaaggcg atcatctgat gagtaggaac
     8701 gggcgctgcg aagtgattaa agaatagact gtgtgtgcgt cgatactcag tgccgaaact
     8761 ccgggattac gcggtggccg cggcaacttt ctccatgacc tcgtcggaga tgtcaaagtt
     8821 cgcatggacc ttttgcacat cgtcgtgctc atccaggatc tccatgagtt tcaacatctg
     8881 ttccgcggcc ttctcctcta atgcaattgt attctgggga atgaagttta attcagcaag
     8941 ggtatactca attttcgcgt cgctcagcgc tttcttgacg gcttcaaaat catgcgggct
     9001 ggtcaggacc tcgaacgcct tatcgctgac cttcacatct tcggcaccgg cctcgagtgc
     9061 aatggtgaga agcgcgtcct cctcgacctt gcccttttcc acgaccagca cacctttctt
     9121 atggaattgc caggcgacag ccccggcctc cgccatgttg ccaccgttct tggttagcaa
     9181 gcttcggatt tcagccacgg tgcggttccg gttgtccgag gtaatttcca tcaagacggc
     9241 ggtccctccg ggaccatagc cctcgagcgt gaactcttca tatgtcacgc cgggcaattc
     9301 gcccgtgccg cgctggatgg cctttttcat ggtatcgccg ggcatgttgg cttccttggc
     9361 cttggcaata gccaaccgca ggcgtgggtt accatcaggg tccccgccgg accgggccgc
     9421 aatcgtcaac tcacggatga tgcgcgtgaa gaccttgccc cgtttggcat cgaccgccga
     9481 tttatgccgt ttgattgttg cccaatgact gtggccaccc atggcgactc ctctttgcgc
     9541 tcacagaccc cgttgcgcat cgggcctatg gacgtgcacg cgaattaaaa atgtacagct
     9601 aacatagtac cgacaagggt gtcaacgcag cgtctatccc tgcgcggccg cggcgacggt
     9661 gcggcctact ccgaataaaa cagcagttgg acgccgatga cgagaacgag gatcgcccag
     9721 acgagttccc agtatgatcg ctccgtcgcg agctgtctct ggcgccagcc ggagatcagt
     9781 tttgatgatc ccgcaataat cgccatggtc cccatcatga gatgatgtag cgcaattttg
     9841 tccgcggcag ggtgatcccc gtgggaatgt gagaacaaca tgaatcctcc cacgacggcg
     9901 aagatcggaa gaggcgcggt ccacaccgcg gtggcggacc tgcccagacg ccgcagaaac
     9961 tcgatggtac ccactgctag agccaggacc ccgtaggttt tatgctgcaa tacctccgga
    10021 tccttcccga aaaacgtctc ggcaaacgtc atcgacccga tcggccaggc atcgtgatcg
    10081 ctccaaataa ggagaaacag gccggccaca gccagcgctc ccggaagtaa aaagcgcgtc
    10141 caggtgagca ccggccatcc cagcgcctgg cgtaactcgc tcagcccgat cagcagaaga
    10201 aagacgccgg ccaagtgatg attgaattca gaataggcaa ttccttcacg cgatccttcc
    10261 cattgagaag ccgacggcga ggattcgtgg tgatgcccgt gattctcggt ggaggagtgg
    10321 ccatggtcgt cagactctgc aatggaaaaa gaccccgtcg aggcgagaac cacgaagcaa
    10381 cacaaggcta gcagcgcaca ccaagacctg agaagtctca gacaataatt cggtcgagag
    10441 agggaaaccg gcaggaatcg aacggcccat ccggagtgcc ggatgggccg aaaactcgtc
    10501 tcatgcatgc attcgtcgtc tacatgaact tcatgaactt ctctttggct tcatccatca
    10561 cttgcgccgt gatctcaacc aggccccgct ctttggcaat tctctcgatt tcccggcggg
    10621 ccatcgggcg aatgaagtca ggaatgttat ccatccgctg ttcggcatcc ttggtccagg
    10681 cgataccgtt aggtgaatcg ttcttagaat catccatgac ctgcagggta atactcttat
    10741 acccgtgctt acgcgcatag gcctcgacac tgctttgaac catgggcttc acgaatgacg
    10801 gcaaccgctc caatttttcc ttggcatccg ccgtccacgt aaattcgctt gtgccgtttc
    10861 cattctgcgg cttaccgccg gaggtcaatc ccatctccgc caccatcgcc gagaacggac
    10921 aaccgcccga ggtcttttct ccaccagccg caggggccgt ggccttcggt gctgccgtcg
    10981 ccggctggcc accttgaatc ttctcattca gataggctgc catttgccca gcaccagcct
    11041 gtgcttcatc cttgagggtt ccgcgagtca tttcgaatgg ttctgcggtt tccgtgcggc
    11101 ctcccaattt aactccaagc gacgcgacca tttgtgtttc gccgggattg gtcaccatcg
    11161 aaaacttggc ttcacaggat ggacagccga agaacacacc gagtgagccc tcaccgggtt
    11221 tttcgacctt ttgaaacgtc atgtaggtct cgcagttcag gcaaacgaac ttcataggct
    11281 cccccttcca ggccggtctg cttacagctt ttccgccagg acttttttgt aatccagcaa
    11341 cgattgaatc cgcccgacaa tctcttgata tttccggatc gtcggatagt cgggatcaag
    11401 gagcggctgc cccttatcga acgttttcgc gagcgtgcga tcaaagggaa tacgacccag
    11461 caagggcaga tccaacacct cgcacatcgc ttcggtattt ccttcaaaca gctcgttttc
    11521 agcgccgcag gacgggcacc gatattcgct catattctct acgataccca acacccggat
    11581 acccatatcc ctggcgtagg tgactgattt ttgcaccaca tcggaggcaa cttctgaagg
    11641 cgtcgtgacc acgatcgcac cggccaggtc gggaataaac cctgcgatga ccggtggctt
    11701 atccgcagcc gctccaggcg gcaagtcagc cagaagataa tccagctcac cccacaccac
    11761 gtcagcaagg aactcccgaa tcacgttcat ttccatcaag cccagccaga ccggactcaa
    11821 gtccatgggg cccttccagc gaacaggcga ggcgtcaccc aggaaaaaat ccatcgacgc
    11881 gaccttgatg ccgagcggcc cgacaggggg ttgggctcct tccggagtca tggtcagcga
    11941 ctggccatgc agacccatca tccgcggaac gcaagggccg ttcaaatcga catcgagcaa
    12001 tccaaccttc gccccctggc gcgcaaacgc cagggcgaga ttgacggttg tcatgctttt
    12061 cccgactccg cctttgccgc tcatcaccac cagtttctgc ttgatgccgg ccatgcgagc
    12121 ctgcacctgc ttcatctgcg cggtgatctg ttgcacgact ttggcgtcgt cggaatactg
    12181 gagttttgtc aggatcgact taagatcctt cccggcagcc atggtcatga aaaaccctcg
    12241 aaattgttga tgaaggacgc taccacaggg gtttcggtgg agtcaaaccg ccaccaggcg
    12301 ccttcgtcag atcgaatact gaatgttcga tttggtcccg gacggctgga tcagcacgcc
    12361 tcgcttcttc aattcttcaa tgatttggcg tgcggcggca tcgaaatcct gcggcccggc
    12421 aaaatgcaag tcggtattgg gcggaggctc ttccccggct ctgctcatgt gcacggcaat
    12481 caccggtgcc ggctgaacca aagttcgtat catctcggcc atgcgttgat aattgatgcc
    12541 gaatgttttg ctcgtcgaga ccacaatcag ccccgtgtcg atcaacaacc gcgcgacttc
    12601 gccatatcga cgaacccgct cggacgcaaa cgacggatcc gcggccgata aatccgcatc
    12661 caacccctgc agcagatttt caccttccaa taaataggca tgccgactat ccgcgaccaa
    12721 caatgcttcc acccgccgcg cgagaaacgt cttgccggtc tgggccgagc cggtaaagag
    12781 cacaatggcc gcacggtggc cgtactgcag cgcccggtct tctggcctga cgtcacccgt
    12841 gagccaggca tattcccgct gacgtgcctc ttcacgtaga ccttcctgtt cgtcgtgcac
    12901 caattcggtg atgatgccgc ctccggaaat gtcatattcg tccaccagca cgaaacggcc
    12961 tgtggattcg aacgaggacg aaaggtcaaa ggcgagcgga gatttcaccc gcagggtcaa
    13021 ttcagccacc tgatttctgg ccaccgattg gctctcctgc agctgcgcca ggtcagcagt
    13081 gtcgataatg cgatggatga ctgccacctc gcaggccacc tcccgagtgg ccaaacgcaa
    13141 cacatatttc cggccctttt ccagaggccg tctgcccatc cagaacaaat tcacacggac
    13201 cgctgtcgaa accagcggca ccgaatcccg atgcgacgcg atctctccac gctccacaaa
    13261 gatctgttcg tcgagtgtaa tgccgaccga ttggccggcc tgcgcctcgc tcggaggcgg
    13321 atcgacattg aacccttcga tggattgcac caccgcggtt ttattggagg gagaaaacac
    13381 gagctgatcg ccgaccttca accgacccgc cgtaatgcgt ccggcgagaa ttcttcgtgc
    13441 atcaaacttg taaacatcct ggagaggaaa gcggagcggt tgctctgaac gaacctgctc
    13501 tttcttgaag agcgccaggg tctccaagac ggtggggcct tgataccagt tcatatgggt
    13561 actgcgcgtc gcaatgttgt cacccatctt ggcactgacc ggaatcattt gttgcggtac
    13621 cgccctgaac tgccccagga attcccgata ttctttttca ataccgtcga acacatcctg
    13681 tcgataaccg accaggtcca tcttattgac gacgaccgcg aattgggtca cgcccaacag
    13741 cgatagcagg tatccatgct tcttcgattg ttcacgcaca ccctctaggg catcgatcag
    13801 caagagggcg gcttcggccc gggcggcgcc cgagatcata ttctttaaaa attccttatg
    13861 ccccggcgca tcaatgatga tgtattgccg cccgttccag ataaagaacg tgcgcgccgt
    13921 atcgatcgtg atgccctgtt cctgctcttc cagaaacgca tcaaacagaa atgcgtactc
    13981 gaactccttg ccctgttgcc ggcagatcgc ctgcaccttt tccaacttgc cttctggcaa
    14041 tgaaccggtg tcggcgtaga ggcgccctac cagcgtagat ttcccgtgat cgacgtggcc
    14101 gacgatgacg atattgaggc tttcttgcgg tttggtctgg tcgtgctgtg tcatatggtt
    14161 actcatgcaa tggaactctc attgcccaat ttcgtctgat accggcagcc ggacggcgga
    14221 aaccggactt acatgtagcc cttcttacgc agcatctcca tgcctcgccc cgcatcctgc
    14281 gcacggccgg cccgttcagc caccgtgctc ttgcgcagct ctacaatgat ctcgtcaacc
    14341 gtcttcgcgg tcgacttgat gggcatggtg cagggggcac agccgaggct gcgatagcgt
    14401 gtgccatcgc ccctgtcgag atacagatcc atgaagggaa tgttctcgtg cttgatatat
    14461 tcccagatat tgatctcggt ccaatcgaga aggggatgaa tccggatatg tgtccccgga
    14521 gggaacgtgg ttttgaactg atcccacaat tcaggcggct gatcgcgaaa atcccaatca
    14581 ccgtgtttgt cacgcggaga aaagtatcgc tctttcgccc tggtgctgtc ttcgtctgca
    14641 cgaacaccga gaataatgcc agtatagcct ttttcctcaa cgagattctt catggcgttt
    14701 gtcttcaacg ccgtacagca gacatcccgc cccaacgtat gattcattcc cgcggccaac
    14761 gcttccttgt tctggcccac caccagattg agccgccatt cgcgcgcaat ccggtcacgg
    14821 tattcgatca ttgccggaat cttgtaactc gtatccacat ggagcaaggg aaagggcaca
    14881 tgcccgaaaa aggccttgcg agcgagccac agcaagacgg ttgaatcctt ccccatcgac
    14941 cagagcatgg cgaggtggtt gaagtgctta taggcttcgc gaagaatata gacgctttgg
    15001 tcttcaagtt gacgcaaatg tttcatgatt cacactcgtg tatttatgcg gacaggccga
    15061 ccttcgaccc cgctgctgtt tcggaccccg gccccgcgat cagttctgac agtttggctt
    15121 ttgctcgacc ggactccaac gcattgcggg cggccgccag attcccggtg atcgacgtcc
    15181 cctttccagc cgcatacagc aacatggcgg cattgagtag gacccagtct cgttgcccgc
    15241 cctggatctc gttagcgacc aatcgcttga tcaggtccgc ttcccgttcg cgctgctctg
    15301 ccgggaaacc tgccatctcg cgaaacgtcg ccatggccag cccggcgtct ttaggttgaa
    15361 acgtaaaggg cgtgatgcgt tcgtccttga gttcgaggag acgggttgag ttaccgatcg
    15421 aaagttccgg gtctccttcg acgccacgga tcaccagcgc gcgcggacaa ctcaacatcc
    15481 gcaaggcctc gatagttttc tcgaaatacg gcggatgcga caagccgatc acctgggaag
    15541 cggcacgggc cggattcagc attctcgcca ccgggtggaa gaaattgcgc acacccaatt
    15601 cctgccgcat ttccagaaac cggctcaccg gcgggtgata cagcgccaga tccagatagg
    15661 caaacccctt cttttccaat tcaggcccga cgagcttggc cgtctgatca gtcggaatac
    15721 cgaggagctt taagaccgag gacacgccgc ggcgatcagc ggggccatcc acaccgtgca
    15781 gcaacaccac ggcgccggct gcagccgcaa tgatcgcggc gggcacaatg gcgtgaaacg
    15841 tttccctctt tcccgcatag acgggcacat ccacgacacc cagtccggag cgcaccggca
    15901 ccggaggaac atattgtctg gcggtggcgg tgaacgcagc taattccgtg accgactcgg
    15961 acttgaaccg catggccgtc agaaacgcgc cgatttgggc ctgcgtggca gttccttcga
    16021 tcatcaggcg catggcctgc ttggcttctt cccaggtgag atccttggag gtcttttgac
    16081 ctttggcgac tttcgcgagc agatgttgca tgtgatggta ttaaccttgt ctcgtatcag
    16141 gatgacgatg atgttattta tgtaatccgc attcggtctt gccgaaattt ttccaccgac
    16201 cggaacgcgg atcgtccccg ggaaggactg gcgcggtgca gtgcgtgcaa ccgatgctgg
    16261 gataattgcg gtcgtgcaag atgttgtacg gcacctcgtg gagctgaata tacgtccaca
    16321 cctgctccgt actccaggcg gccaacgggt tcactttgac gagctgaaat ttcttgtccc
    16381 actcgatcag tccggcgttg gcccgggtcg gagcctgatc ccgcctgatt ccggtgatcc
    16441 acgcgctgta ctgaccgaga atacgggtca atggctcgat ttttctcaac tcgcagcatt
    16501 gatcgggctt gctggcccat aaggccgctc catgttgggc agcctgctgc tccggcgtca
    16561 agagcgattt catctgaatc acttgcgccg gtttcagacc atatcgggcc acaatgcggt
    16621 cgcgcacgtc gaacgtttcc ggaaatagaa agtcggtatc gaggtagaac agcggcgcat
    16681 ccggatcgat ccgatgcacc atatcgacca acgctacatc ttccgccccg aagctacagg
    16741 cgagcacgat cctgccctga tacgtcttca gggcatacgc cagcaggtcc cacggctgct
    16801 gagattcaaa cgaatcactc agcgccttca gctctgcgtc ggaaggtcgt tcagcggact
    16861 tctgtgtgtc ttgcactctt tctcacccct cgaccttttc caccaaaatc ttgaaatgtt
    16921 tggcctccgg ctccaacggg ccttcttcaa gaatcttgtg cccatcgttc cgtagactca
    16981 tcggcacatt gcgaatcggc tctccggcat cgagccagac ttcgagctgc tcgccattgt
    17041 ccatcatttc caatttcaat ttggtcttca cataattcat cgggcaggcg acaccgcgca
    17101 aatcgtagac cggcgcggtg gcaacggggc tcgcaggctc cactttggga gccggagtcg
    17161 gaaccacctc ttccttcacc tgcgccaact tcaaatcctt gcccatttgt tcggtggccg
    17221 cccggcagac tgagaggaac cgcttggcaa acgccatctt gtcattcgcc gtttcggccg
    17281 tggcatcctt ggatcccaaa tcccccacct gagcaccgag gttggtgtag gaggcgggta
    17341 caatgccttt gctcgccaag cgcacgtcga actcctggaa tgtctccgca tcggtcgccg
    17401 gatcgattcc ttcggtgacc aacaaccctt tggccgccgc caggaccgcg cgataggact
    17461 tattcacgga cagagcgtat tgatgcttct ccacaagtag tttcgcttga tagagctcct
    17521 gatcggcttc caacatgcgg tcgtcgatca tttccaaggc tccacctgcg cattcgccgg
    17581 gccccagatc ctccaacaca aattcggcct caccttccca gtcatagtag taggtcggat
    17641 cctggtcgta cgcaggaaca atcgtatagg ggatgagttc gtccttgagg acgttttttc
    17701 cgacccgccc gatgaaggtt tgcaaacttt cccccggctt ccggtcccgg cgatacacgt
    17761 cgaccagatg gcggaccgcc gccggaacac ttttcgccgg caacttcacc gcaagctgcc
    17821 cgattttcgg cgtgccgtcc acgtgcccgc ccaaatgcag ttcgtaatgc ggcgccgtat
    17881 gctcaccgag ccgcttacca actccgtgca ggccgatcgt cgcaatatgg tgttgtgcgc
    17941 acgagttatg acagccgctg attttgacgc tgacgtcgcg cagatcttcc ggcacctggc
    18001 cggcaggaaa gacctccgcg agcgctcggg ccatcccctt tgacgaggtg atccccaatc
    18061 cgcacgtatc cgtacccggg caggcaatga tgtcctcgac gagctcagcg cctggatcgc
    18121 ccaggctatg cgcgacgagt tcctcatgaa acgcgctcaa acggtcctcc tgaatccatc
    18181 ggatgatcac gttttgattg atggtggtcc ggatgttgcc attggaatag cgttcggcca
    18241 aatcggcaac cacccacatc tgttcgcccg tcaaatcgcc catcggtaac ttgatggcag
    18301 ccgcagcaaa tcccgcctgc cgttgaggca ccacattggt ccgcttccag gcctcaaacg
    18361 ctgattcggc gccattccgc gatgcggcgc cattgccgtt tccattccca tttccgttgg
    18421 tgctgttcgg tgctttcgtc ggcatcaaca acggcggcgt atcggcatac tcgagcagtt
    18481 tgatcggttc atgcgtcggc accgcatatc ccatggccgc ataggccgcc tcccagcgac
    18541 gtttgaattc gtcgaatccg agtttctcga tgacgaactt catgcgagcc ttgttccgat
    18601 tcttgcggtt tcctaaggta tcgaacactt tgatcaccgc ttcgatggtc ggcagcaatt
    18661 cgtccatcgg cgtaaattct cgcagcactt gcgccatgcg cggtgtcgat cccaggccac
    18721 caccggccac catccggaag ccaatggttc catccgcccg tttcgccgcg agcaggccga
    18781 tgtcgtgaat gggtgtgagg gcgcaatcct gccggcagcc cgacagcgcg atcttgaatt
    18841 tccgcgggag actctggttc agcggattac gcagcagatg gtaggcaact gttttcgcgt
    18901 agggcgtcac atcgaacact tcgccctggc acactccggc cagatggcac gccgtcacat
    18961 tacgcaccgt gttggcgcag gcttcacgtg tggtcaatcc cactgccgcc aactggcgca
    19021 tcatctcagg cacgtgctta agctccacga aatgcagctg aatgtcctga cgcgtcgtga
    19081 catggcccac gccggtggca aactggtcgg ccaattcagc cacgcgtcgc aattgattgg
    19141 ccgtcaaacc gccgaatgga attttgatcc ggaacatctg cacgccgggc tgccgctgac
    19201 catagatgcc gtactgcaag cggaagggct tgaagatgtc ggtcgagaga tcgccggcca
    19261 gcacacgttg agcttcggcc tcgaatgtct cgatttcttc gacaatatgg ggaggaatgg
    19321 gctcagtctt cacagcgggg cgaagagcct ctgtatgact acttatcata acgacctcgt
    19381 cggttcggaa gtaatgtgca gggcatcatg cgggagttca aatgtgatac atcaatgtat
    19441 gttgctcgtc gagggcccgt tggcgctttg ccaactcctc gaccgtcact tcggataaca
    19501 cgctgagttc cgcccgtttc acacgatccc aaatatgcgc aaggagcgcc tcttgcttgg
    19561 aagctcgtga cgaggtctgt ttcggagcgg cgtctccgtt cgtcgacaga agcgggcctt
    19621 ctaaggcatc gagaatgtcc gccacagaga gctccgaggg ttttcgactc aacacatacc
    19681 cgccttgcgc tccgcgctga ctcgtcacca ctcccgcctt cttcatcgca tgcagcactt
    19741 gctcgaggaa ccgggccgga atcgcctgcc gcttggcgat cgactttgcg cacaccggat
    19801 gctcggcatg gtgaagcgca agatctacgg cagcgatgat cccataggtg gctctaaggc
    19861 taaccttcat ttgcgactat tccgatgggg attcatgaat gtctatacat tgagccccgc
    19921 tttcctgtca agcccgcggg cgcaggcctg gttgcggata tcgcggatgc gctggcggcg
    19981 atcacgttgg ccgacaattg ctcgccatct ctcggaagag atgaatttgc tgcgtcgaaa
    20041 gtttctaatt tgcattgcga attgatgcca ccaacaatct cttctaccag tgctcacttt
    20101 ctccactttg atcgatcagg ctgcatcaat accggtgcag atttcctcat tgacatcatc
    20161 tggggcctga gcaataatcc cgccctcttc ttcctcggtg cctctcatga cgctgcgttc
    20221 atcaacattt aatattctat tgctcagcac agacagcgag atccagtcac actacaagga
    20281 ccttttcggt gagcaggcga ttaccgttgg ccgtgaaggc ccatcactgc agaaggacct
    20341 ggcaaagcgc gactacgatg cggtcatcat cgaatcgaag ttggctgccg cggcggactt
    20401 gagcaaattg ctgaccggaa tcgatccttc acgaaccctg ctcctcgtgg gatcgcgtac
    20461 cgtgctgaaa cgcaccgccg ccgtgcttca ggcgtccaag gcctcgaagg ccgaagcgcc
    20521 tccgtcgaat ggcaaaggac aagctttgtg tttggattct tatctcgaac acaaggtcgg
    20581 ggatttcgtc aaaggcatgc ggaatggctc agggaaaaat ctccacccga tgctgatcgc
    20641 cgcggtcgaa cgcccgttga ttcttctgac cttgcgagag accaagggca accagatcca
    20701 agccgcggag ctccttgggc tgaatcgcaa tacgttgaga aaaaagattc tcgatctgga
    20761 tatccccgtc aaacgcgcca gggccagcta gcgtaccgac gactgagccg cacctcacaa
    20821 ggcgtcataa gtacgcccat gcatcacatc cacgggtcgc caaccaccaa cggagggaag
    20881 caatgaccaa ggaagaattg attgcacaaa tggcgaacag tgccggtatc accaaggtcg
    20941 ccgcaacagt cgcgctcgaa gccttcaccg gcgcagtcac cacgtcgctc aagaagggaa
    21001 agcgggtgac gttggtcaat ttcggcacct ttacgatttc aaagcgcaag gccaggatgg
    21061 gacgcaatcc gcggaccggc gaagccttga aaattcctgc cgcccgcatt ccgaagttct
    21121 cggccggcaa ggaactcaag gcggccgtca agtaggagtc ggcagactcc tggacagccc
    21181 cacgggacaa gacgcgattg cgtggctcgg gcacagagaa aagcctgtct tgccaccgcg
    21241 tttccttgac acctttggta agcctgtatt agcatcgccg atcgataggc gcgtagctca
    21301 gggggagagc gctaccttga cacggtagag gtcgacggtt caagaccgtc cgcgcctacc
    21361 attttcgttc gctcatgaag ttcgtgatcc gattacccgc agggggtggt cggcgcgacg
    21421 ttgtattttt ttggcaggat tgaccggagt ggcctcacag ctcattcaca tcacccttcc
    21481 tgacggaact cgaaaacaag taccaacagg atgcaccgtc cgagaggcct taaccccgca
    21541 gggaggccgt ctcgacggga aaatcttggc cgcgaaggtc aacggcgtgc cgatggactt
    21601 gttccatgcg ttggatcaag acgcgtccgt cgaaccgttg acctttgaat ccgcggaagg
    21661 gcgtgaggtg taccgccata gcagcaccca cattatggct caagcggtca aggaagtgtt
    21721 cccgagtgcg caacttacga ttggtccggc gctcgacgac gggttctact acgattttgc
    21781 gttcgaccgg ccgttcaccc cggaagatct ggaaaagatc gaagcccgcg ccattgaaat
    21841 caccaagcgt ggcctgactg tcagccgtag tgaactatcc aaagaagacg cgatcaagtt
    21901 ttttcaggat cgcggggaag cctataaggt tgagctaatc aatagctttg acgatggttc
    21961 ccctatttcc ctgtaccgtc agggagaatt cgtcgacctt tgtcgcgggc cgcacctgcc
    22021 caccaccggc tacgtgggcg cctttaagct cctctcgacc ggcggcgcct actggcgcgg
    22081 ggacgaacgc aatcccatgc tccagcgggt gtacggcacc tcgtttccca cgaaaaagga
    22141 acttgatgcc catctggcaa aactcgaaga gatcaagcgt cgcgaccatc gcaaactcgg
    22201 caaagagctc gacctgatca cgattcaaga cgacatcggt cccggcttgg tgctctggca
    22261 tcccaaaggc tccctgatcc ggctgctcat cgaaaatttc tggcgggagc aacacatcaa
    22321 ggacggctac gacctggtgt attcgccgca tgtcgcgagg ctcgacctgt ggaaaacgag
    22381 cggccacgtc gactattacc gcgaaaacat gtttgcctcg atgaaactgg aaggcagcga
    22441 gtatcagctc aagccgatga actgcccctt ccatatcatg atctacaaat cgcacttgcg
    22501 cagctatcgc gatttgccga tccggtacgg agagttgggc accgtctatc gttacgagcg
    22561 aaccggcgtg ctgcatgggc tgctacgggt ccgcggcttc actcaagacg acgcgcattt
    22621 gttctgccga cccgatcaaa tcgaggccga agtcagccgc gtgctggact tcaccttttt
    22681 cgtgcttggc accttcggat tcacggaatt tgaaatttac ctctcgacca ggcccgagaa
    22741 atccgtggga tcggatgaga actggaccat cgccaccaac gccttggaag cagcgctgaa
    22801 gagccgcaac gtggcctatc aggttgatcc gggtgaaggc gtattctacg gcccgaaaat
    22861 cgacatcaaa attaaagatg tcctgggtcg atcatggcaa tgctcgacgg ttcaggtcga
    22921 tttcaacaat cctgaacgct tcaagctggc ctataccggc gaagacggta aacaccatca
    22981 accgatcatg attcatcggg cgctcatggg atcgatcgag cgcttctttg gcattttgat
    23041 cgagcactat gccggcgcct tcccgacgtg gctggctccg gttcaagccg ttgtactgac
    23101 aatcaccgac catcaacagg aattcgcggc taagatcgtc tccaccctca agagccacgg
    23161 ctaccgtgtt gaagcggacc tccgcaacga gaagatcggt ttcaagatcc gcgaggcgga
    23221 aaagaacaag attccctata tgctggtggt gggcgataag gaagtgcaga gcggcatggt
    23281 ggcggttcgc ggtcgaagca aagctgatca tgggaccatg ccgatcgaac aattcctgga
    23341 actcatccgc acagacacga atcaatcatt acgcgataca gcaacccact cacaaacgag
    23401 gtgacctatc gtccctaaat tacgtgtaaa ccgggaaatc aggattcggg aagttcgtgt
    23461 catcggtcct gacggcgagc aattgggaat actgccgacc gtagaggcat tcaataaggc
    23521 tcaggaaggg ggctacgatc tcgtcgaggt ggccccgacc tcacagcctc cggtttgccg
    23581 tatcatggac tatgggaaat ataagttcga gctcagcaag aaagatcacc agagccgtcg
    23641 gcaccagaaa tccacgcagg tcaaagagat caagctgaga ccacgcaccg acaaacacga
    23701 tctcgagatc aagatccgcc agatcaaaga gtttttggcc gatgggaata agaccaaggt
    23761 gacgctcacg tatcgtggtc gtgagatggc caatcaggaa atgggtcgca cgatgatgac
    23821 gagtgtgatc cagcagtgca tggaagcggg gaccgttgaa tttgcgccga ggatggaagg
    23881 gcggagcctg attatgattt tggctcccaa atagtgtgcg cgacgcacgc aaggaaggaa
    23941 tgagagcatg aagcagaaga tgaaaaccca cagcggcgcg aagaagcggt tccgacgaac
    24001 gggaacgggc aaattggttc gccgaaaggc cggcggtcgg catttgttga ccggcaagcc
    24061 acgcgaccgc aagcggaact tgaagggggc cacggaagtc tcgtccgcct cgacacccgc
    24121 gttgaacaag ttgcttccgc agtaacgatg attgtgccgt gaacaggatc tgaaaggagt
    24181 agcgtcccat gcctcgtaca aaaggtggtc cgaaaacacg acagcggcga aagaagcgcc
    24241 tgaaactggc gaaaggccag tacggcgcca aaagccggct gttccgaaca gcaaccgaat
    24301 cagtcgataa agggcaggct tacgcgtatt cagggcgaaa gcagcggaag cgtgatttcc
    24361 gccagctctg gatcgcgcgc atcagcgccg cgacccgctt gcatggcatt gcgtatagcc
    24421 gcttcatgaa cgccctgaag aaggcgaata tcttattgga tcggaaagtg ctgtccgata
    24481 tggcgatccg ggatatggcg gggtttgaga aactcgtcgg cgttgccaag cagcagctcg
    24541 ccaccgcagc gagctaacgg gttcgcctca atgctctgtg acgggaagat cctgatggaa
    24601 cggattttcc cgtcacgctt catccagtaa tcgatcgatt tccaactcca gcgcgactgc
    24661 cggcactcca atctgccact gctctaacac ctcgggatgc aactctccga gcagtccgat
    24721 agctcgtcca tccagcatga ttctgcctgc cctgccctca agaaacgacg ggtgagggac
    24781 cggttccaac ctaaaaggac ggtccaagta atagagcagc aagtccaggc aggagtgaat
    24841 ttcggaaaaa tgcgcgccgg catgggcgat gaccgccccc aacatcgtga tcgtccgtga
    24901 accgacatcg gcgcgagcat cggggacagc cacctcaccg acctcgaaca tccgatgcgg
    24961 ataaaacgcc cggctcgaat tggtctccac ttgcaacaac gaaggggtga tccactggcg
    25021 caggcacgaa tagctaagcg acatcacatt atccacttcc acgacgttcg cccactcnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtg tagccgtagg acttgagcgc
    25201 cgtggtgact tctttgctac ccaacgcttc tcccagcgcg gtttcgatag ccttgagcgg
    25261 aatcgtccgc ggcgcgccga aatcgatcgg tgtgtgccag cgcttaccga aggcggtttt
    25321 caccggcgac tgaatttcaa ccggcgcaat gacggctcca cggtcggcca ggttcgcggc
    25381 aaaaatgttc aatgtcagcg cgaccatgtg aagatcagtc ccggtcacct ctaggaacag
    25441 atgatcgtct ccgacctgca cctcgccgat ttcacggcta ttgatgatcg gcgggaacga
    25501 caggacttgc cctttggcat cacgcagtat cggtaatcga tcgtggccgc tgacgatacc
    25561 gccatactcc acacctttcg gatggaccat caggatctcg cgcagagtca tcaccgtttc
    25621 catgcccaac ggggtgaacc gcacttcatc cggcttcacc agatcgtacg atacgggaaa
    25681 gacaatcggc gcgaggcgat agaggccgat ggacaccgtg cggcgcttgc gcccgaaaat
    25741 atccgccagc ttttcctgcg tctggatcag ttgcgccaga ccggaggccg tcacacgata
    25801 gccgatcgcc gtacaggccg ccacatacgg ccgcacctgc tccagcccgg gtgccacgac
    25861 gagttttcca gcggcacgtt tttgcttctt gaaaaagggg taggaactgg ccgtgccctg
    25921 ctgcttggtt ctgatctgcc gggcaatccc ttcgcaacac cacaaatccg gccggttgct
    25981 gtcctgcagc tcgattcgta attcaccggt ttcgatattg tgccccttca actcgccttt
    26041 caccaacatc agccattgct caagctggtc gatcgaaata cgggacgccg tttcgccatg
    26101 accggcgatc aacgcctcaa gatcgtctcg ttgtagagaa atggtgggca tgatgtgatc
    26161 gtcctaaaaa cttccgcgca tggtccgaat gaattccaaa tccgccgaaa agaggtcgcg
    26221 aatatcgtga atacccaacg ccaccatcgc catacgatcc agcccgagtc cccaggcaat
    26281 gaccggcaca gagactccga gcggcgtcgt gacttcaggc cggaacaatc ccgcgcctcc
    26341 cagctccatc cagcccaact tcggatggcg cacatgcatt tcaaccgacg gttcggtgaa
    26401 cgggaaatag gcgggaagaa acttcacttc cttggcctgc gccacttcac gggcgaacag
    26461 gtttaacaat cccaacaacg ttcgaaagtt gatatccgtt cccaacacaa tcccttctac
    26521 ctggaaaaaa tcagtagcat gggtggcgtc cacctggtcg taacgaaaac agcgggcgat
    26581 cgaaaagtac tttccgggga cggatgggtt ggcagcgagc ctgcgcgcag aaaccgccgt
    26641 cccttggctt cgcaacacca accgcttcgc tcgctccgca tcgaactgat agccccaacc
    26701 ggtcgatccg gcccctgcgc cattctcatg gacctgtgtc acattcgtca gatacggttc
    26761 atcgatgttg cgggcatggg tcgggttttt cacaaagtac acgtcgtgga tatcgcgcgc
    26821 gggatgaaac tgcggcatga acagagcgtc catattccaa aactccgtct ccacgagcgt
    26881 tccacgcatc tcctggaagc ccatgctgac cagcttgagt ttcacgagat ccaggaattc
    26941 ccgatacggg tgccgtttcc cggccgcgat cctcggagcc cgcaagctga tcgtatactt
    27001 ccggaactgc ttcgtccgcc aggcaccttc cttgagaagc tcgggcgtca attgggaaac
    27061 ttcttctgcg acgccgtcac gcgacaactg gtcagccact tcctgaccgg aaggcgtcag
    27121 cagaaacgat cgcgtcaccc gttcgtcgac ccggaagggc tctcgcgcgt tcccacgttt
    27181 gaccgcatgt tgctgaagca cagcccggag cggttccgca aaggtctgca actcacgttg
    27241 tccgcttcgc aattcctgca agagggcacg catggcctct gccgtggaac tattccggcc
    27301 ggtgctttcg atgcagccac cctgtacgat gagaatggcg ccttcttttt tgagggtgcc
    27361 gaccgcttta ctgacatccg acggttcaag cacttccttg gcctgaatgt cctggatggt
    27421 cagacgtttg ccggtctgcc ccgcttcccg tgccgccgac agcacgcgtt caatcggcgc
    27481 gtatttctcg aaaaacacct caccgatcgg cgtcaacgac gcaatatacg caaccgtttc
    27541 ggcgtgaacg gcaatgaggg atttggccaa gagccattcg acggccatac ttaactgcga
    27601 aggttccagc ccggtggagt ccgcgagttg atcgagcgtg gagggcgagc caggctgcgg
    27661 agccaatgcc agcagcactt tgctttcgag gggatgaaga ttatccatga atgacggtgc
    27721 ggtccgatgc ctctgctatc gctggccggc catagttctg ctgctgcgtg gcggtgatac
    27781 ccgaccggcc ctgagggctg taatcgcggc ggcataatcg tgatcggaaa atacggcgga
    27841 tcctgctacc aacacatcgg cccccgccgc aagaatggaa tccgcgttat ccggtttcac
    27901 cccaccgtcc acctcaagaa gcgcatggct gtgggtccgg tcgatcatgg cccggactgc
    27961 ggcgaccttt tgcaaggacg agggaataaa tttttgcccg ccgaatccgg gattcacgga
    28021 cataatgaga atgagatcgg cgtcgcccac aatttcggaa agggtgatcg ccggtgtggc
    28081 gggattcaac gtgaccccgg cctttacgcc tcgttctttg atcaattgca cagtccgatg
    28141 caggtgagga caggtttcca cgtgcacggt cagatagtcc gcgccggctt cggcaaactc
    28201 cccgatgaac gcatccggat tggtcatcat cagatgcacg tccagcggca ggctggtcac
    28261 ttttctcaac gcttcaacga ccggggggcc gatcgtcagg ttcggcacga agtgaccatc
    28321 catcacatcc acatgcaacc aatcggcgcc cgcttcttcg actttggcga cctcttccgc
    28381 cagacgtgcg aaatctgccg atagaataga tggcgcaata cagacggtcc tgccagccat
    28441 tacccaaccc tccgcagacg ggcggcaaaa aacgcatcca tgttatttct attggccatc
    28501 gtagacagat cccctcgagg ggtcagaaac ggcagaccgg ctgggggcaa ccagggcgcg
    28561 atcgaatcgc gctgaaattc acgatgagac tgacaaaact cgtcgatgac cgactcggtt
    28621 tcttccggct caatcgagca cgtactatag accaacactc caccaggccg caagagacga
    28681 gtcgtcgtgg cgagcaggtc cagctgcacc tgtcgatgtt gcgcgatgga atccggcgtc
    28741 ttgtaccatt tggcttctgg gtgacgccgc agcacgccga ggccgctgca cggggcatcg
    28801 agtaaaatcc gatcgaacgg gcgcgattgc acctccggca gcgcgggaga gatcgctggc
    28861 tctctcgccc ccgcctcttc ggctttcagc tctcgcatgt cagctacgac tggcgtgacg
    28921 atcgtcacac ccaggcgccg acaattgtcc atcacccggt ccagacgtga tgcgactcta
    28981 tcaaccgcga tgatttcacc tcgattggcc atgagcgcgg cgatatgcgt cgctttgccg
    29041 ccaggtgcgg cgcaggcatc caacacacgg tgctccggct ggacgtccaa gagcagaggc
    29101 accagttgcc cggcttcatc ttcgacataa aaatgtcccg ccgggtatcc agggagatcg
    29161 gcgacgagac tggtgcggcc tagttgaatg ccgacttcgc tgatcgacgt gggctccgct
    29221 tcgacgtgcg ccgcgcccaa gtcagccagt agcgccgctc gcgtggttcg aagccggttc
    29281 acacgaagtg tgagcggagg cacttcaacc gaggcccggc ataaggttgc ggctcgttca
    29341 gccccccact gtcgacacca gcgttccacg agccatgtgg gacatgagta ctgaacggcc
    29401 agtgcctcga caggattcct cgcgagatcc ggccaatccg gaggcgacgc ccgcagcatg
    29461 gcccgcagta cggcattgac gaatccactc caatcgcgcc ccaagcgccg tcgcagctgc
    29521 ttcatcatct gcacggattc gttcactgct gctgaggccg gcacccgatc caggtacagc
    29581 acttgatacg cgcccaagcg caacatgatt tgcacgagag tgggaagttt ggcgatacgc
    29641 cgatcggaca agaggcctag acgccaatcc agcgtcgcgc gataccgcaa cacaccgcgc
    29701 acgagttcca ccatgaaggc tcgatcgcgg agatcgagcc cggccttcgc agaaatctga
    29761 tcgaacaagt catcggacaa cgtgccggct tcctcgatgg ccaataacgc cttgacggcg
    29821 gcatgccgcc cgggagaaac ggcgtgaccg ggtgacgctg gttgcggatc agttcccatg
    29881 ggcactagac atgaacatcg ccggaaatgg tgaatggaat gcgcgtgccg cgctgggtca
    29941 ggcctgtgga ggcggaacag cgtgcagact gataccttca gccacacgat ggcctgccag
    30001 atattgcgcc accgtcatac ggcggctatt ggatggttgg atgtcgagaa tctgaatggc
    30061 tcccgcaccg gtcgcgacat cgatgcggtc tttcgtcacc ttcgtgacag tgcccggagc
    30121 cgcttgctga gatgcatcgc cgaccgcaac gcggcagaga gtccaccgtt caccgttcac
    30181 atagctatat gcgccgggcc agggagaaag tccccgcacg cggttggcga tctcctgggc
    30241 cgatagcgtc caatcgatga ggccgtcctc cttcttgagc aacggcgcca tagtcgcctg
    30301 agaatcatcc tgacgttccg gcgtcaacgt ccccgccttc aggcgacgca gggtctcgac
    30361 cagcaaccgt ccgcccacat ccgcaagccg aggggcgagt gtgccggctg tatcgtctgg
    30421 acggatttct acagcctcac gcagcagcat atcgccggtg tccatccctt cagccatgaa
    30481 catagtcgta atgccggttt cccgctcacc gcggataatg gcccattgca gaggaccagc
    30541 cccgcgatac ttcggcaaaa gcgacccatg gacgttgatg cagcctcgag gcggcagcgt
    30601 cagaatgacc ggggggagga tacggccgaa ggcagtcacc gcaatgacat ccggctgcca
    30661 ctcgcgtaat gcgtccagaa aagccggatc cttcatcttc agtggttgga gcacgggaat
    30721 gccttcgcgc tggcagacga ctttcaccgg ggaaggaatg acctcctgcc cgcggccttt
    30781 cgggcgatcc ggctgtgtca ccacgccgac gacctgatca tcggacttca gtaacgcctc
    30841 caatgagggc acggcgaagt cgggtgttcc catgaatacg atacgcatgc tctggcttta
    30901 tcattctccc ttcgcgaatg caattctcgt cgcggcttcc ttgactctcg tttcacacgc
    30961 ctgttagtat ccggtcgcgg ggtggagcag cctggtagct cgttgggctc ataacccaaa
    31021 ggtcgtcggt tcaaatccgg cccccgcaac caacctttc
//
LOCUS       DAOQDS010000049        30829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_503_length_30829_cov_20.235621, whole genome shotgun sequence.
ACCESSION   DAOQDS010000049 DAOQDS010000000
VERSION     DAOQDS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 30829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30829
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_503_length_30829_cov_20.235621"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1950
                     /locus_tag="PK589_12825"
     CDS             <1..1950
                     /locus_tag="PK589_12825"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ20536.1"
                     /translation="IVYLPIMFFTGIIKYLFVPLAMTVAFAMLADYVVSMSVTPVVLA
                     WLYQGGHGKGTEHEASADEGWFRYVLAIYEPILKGGLRFKPVVIGLATVALIATGAFL
                     IPRLHTEFFPKVDAGNFTMLVTAPEGSRIEKTTAIVGQVEQLVHETIPKADLEEVISN
                     TGLYYGDAARFAPNTGNHTAFVLVNLITGHEGRTEDYIAQLRSRLKTSLPGVEISFQT
                     GGIISDVLNFGLKAPIDIQVKGPSLDVIRPVAERIQQQIAQVPNTVDVRIKQGKSYPE
                     LHIDVDRTKAAYYGINQNRVVVDVITGISSNLALSPNYWLDPKTANGYFLLAQYPEQS
                     LTTTEDLLNIPIIGARTPLLPTSSLTGGGIQGSTLALQNTPFAGRQMEIASGYYASSD
                     DRRGPPVMLRDVAQLKFKTGPDSVDHYDLSRLINVLVTPVGNDLGHVAKDIEQVLAGI
                     QLPKDVTVQLRGEVANMRGAIQNFALALPLAVVLIYLVMVALFRSFIDPLIILVAVPL
                     GWIGTVLTLHVTNTSVNVESMIGTLMMMGIVVSNSILLVDFANRMVRNGSTAAHAVLE
                     AGRRRIRPILMTALATILGLLPLALGFGEGNETMVPLARAVVGGLAVSTIMTLLVVPV
                     MHSLVLIRRERAPLSATAPATSEDI"
     gene            1950..3254
                     /locus_tag="PK589_12830"
     CDS             1950..3254
                     /locus_tag="PK589_12830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ20537.1"
                     /translation="MDEPSTTLRPVRATRRKIRWIALAIVLTLVIGGLLYWREIHDVG
                     HPVPPASPAVPTPPQPPEESVAVPDSAPVDVQVTKPTRRDLVYTVKLPANVSPLYQTT
                     LYAKVSGYLKWIGPDKGDAVKRNDVVAVIDAPEVEEQYQQAVSDYKIKKLTYERLAKV
                     WKESPDVIAKQDVDVAEAAYQGARHLMEQRVVMRDYTKVRAPYDGIVTARFADPGALI
                     QIATSSATSAIPLFTIMDLNTVRIYANVPQDDSPWIVPGKTQATVKVTELPSRSFTGL
                     VTRSTMALDPATRSLLVEIDLPNQDHALRPGTFAEVTLGLREIPQALVVPPQAITSTP
                     KGKSVFVVEDGKAKPVAVQTGISDGRWIEISSGLRGDEDVVAVGKRRLLEGMPVVASP
                     FNLPDAKLSQQKFERRAPGGTPPPPTAAPPTNRAAQTQGDRP"
     gene            3251..4690
                     /locus_tag="PK589_12835"
     CDS             3251..4690
                     /locus_tag="PK589_12835"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ20538.1"
                     /translation="MTYLLLRSTSLMLAALSLAAASASGTYAAEAEQPVPLSRGSFLG
                     VQQAVEIAVKNHPMLIEGQANLKASEARTEQARSLYYPQVYANANTVAGAGVSNPRFL
                     IGGGLLRENQTTFTGGVIANQRLYDFGYTSNLIESNKLAERAQGQDVNARRALVLLYV
                     QRAYLNSLKRHRLVQIAEETVRERGIIAGQIETLYRQQLKSKLDFDLARVELVNAQSL
                     LVRSRNDLKASFADLNRTMGIAGADDYVLEDISVAVRPQKTLETLINESLSHPEVKRA
                     KEQTASADARLTATKRQYLPTVSAIASGGTFDPFDPRQNQQTGGWWMAGAMVSMPLFT
                     GFLIENQVVEANANRQAASAATSNIEQALTQQVTNAYLDTLTFAQQITLAEEQVKTAQ
                     EALQLSKQRYKLGLGTVVEVTQSEVAVTAAQTRLADTQYDYKIAEVTLAYTSQGDDTG
                     RILLMSRASTASRPGDAGSQAEGPPAAAY"
     gene            complement(4745..5449)
                     /locus_tag="PK589_12840"
     CDS             complement(4745..5449)
                     /locus_tag="PK589_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20539.1"
                     /translation="MTPVLRFLRVTVHLSQVAVLAAAVFTAAGCAIPQVPSRTVYEDP
                     VNFVRLELDANVLPEWPPGHFTHPARFSQEQVRRVLMGLTVQEHRTMVQRWIGDDSRR
                     LPMFRDAEIMILVPQLVEALRLARENERVTYYLSQPQTSVKRTITSGGMYIRGTELHF
                     ILGNWQTLYGIPAYGMIYDRRYPMNPIISKGFDLFFDLDQALVTQTTSIWDGLLANTK
                     DELVIDLAIVFPGQNI"
     gene            complement(5488..5997)
                     /locus_tag="PK589_12845"
     CDS             complement(5488..5997)
                     /locus_tag="PK589_12845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ20540.1"
                     /translation="MTAQTGSPQATITVTSKNEPWGQIVLRFFPDVAPNHVKNFTDLA
                     KKGFYNGTTFHRVIPGFMIQGGDPNSKNPDRASHGMGGPGHNVKAEFNSKPHKRGTLS
                     MARANDPDSAGSQFFICVNDANFLDWQYTVFGEVQSGLDVVDKVVGAKRDGRDNPLER
                     VEMTVTITE"
     gene            complement(6015..7091)
                     /locus_tag="PK589_12850"
     CDS             complement(6015..7091)
                     /locus_tag="PK589_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20541.1"
                     /translation="MLRHMLVLTALTVGGLQGLVHAAPSLIGNEGGGALKLEQGHPGQ
                     DPSSPADIGNTSIQALASGAGGVIYAGSFGMGIFRSTDRGGTWMSANEGLTDPFVLCL
                     TTAKDGAIYAGTFRGGVFRSRDAGKTWQPINKGLKRLEIKVLMMDAKGLYAGTGDGVY
                     LLNAAQDHWKVVTTGLDEILVHALARTDDGTLYAGTSGKGVHSYKERTPGWTRLRQGL
                     KDHEGLVENFIRVLAVDKEQGVYAGTFDGGVFRSGDGGKTWLPISRALPNDSIRGLIS
                     NERGVYVATGRGIFKTVDKGRQWMPLNKGLSNLSVQVLIEGDKGGFYAGTSSGVFRSD
                     DDGLTWTAVSQGLEGESVAPFKFN"
     gene            complement(7126..7896)
                     /gene="tatC"
                     /locus_tag="PK589_12855"
     CDS             complement(7126..7896)
                     /gene="tatC"
                     /locus_tag="PK589_12855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNJ20542.1"
                     /translation="MLAPLAAHIQSLKKRLLIIAVTLGVAFASAFAYSTEMVAWLNRP
                     FPNQLVFYGPTEALFASIKVSFLAGIILSLPIVFYQFWKFIEPALLPKEQRWAIPIFL
                     LAALLFALGLIFCNLVILPLVIDFFVSFGMDRDITPALGVGTYIDFNVKFLLIFGCAF
                     ELPLVLTILSRVGVVSAAVLAHYRKHAIMAALIISAIVTPDATLFTMLLMAVPLMVLY
                     EIGIIGAKIFGRSSGPAPDMNLPLDPDIPIGTAGHRVR"
     gene            complement(7991..8206)
                     /locus_tag="PK589_12860"
     CDS             complement(7991..8206)
                     /locus_tag="PK589_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HNJ20543.1"
                     /translation="MQTETAITERLRRSNTEFRALEESHHRLDEKLADLQRRHVLTPA
                     EEVLKKQLQKEKLATKDKIAELIRSSR"
     gene            complement(8271..8777)
                     /gene="rimI"
                     /locus_tag="PK589_12865"
     CDS             complement(8271..8777)
                     /gene="rimI"
                     /locus_tag="PK589_12865"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNJ20544.1"
                     /translation="MTSTSILIEPATPAVLDEVLAIEQACFSAPWTRKMLAAELSGNQ
                     FANFLIAKCPDPLSGSFAVAGYFCFWVVFEEVRLMNLAVLEPFRRQGVATKLVCTALQ
                     TGVERGASRAMLEVRESNLEAQTLYRRLGFRQTAKRSRYYNHPEEDAVLMEMTPLQVS
                     EVCRQPGV"
     gene            complement(8805..9629)
                     /gene="tsaB"
                     /locus_tag="PK589_12870"
     CDS             complement(8805..9629)
                     /gene="tsaB"
                     /locus_tag="PK589_12870"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNJ20545.1"
                     /translation="MKVQPDHHLLAIDTATAWQSVALLSGEKVLALVEQDADGSHARS
                     LLGAIDRVLRETSITLKQLQSLAVSIGPGSFTGLRVGLATMLGFRAVLGLPIAPVPTL
                     EAMAWNLRDAKGLLVPVLKSRHNEVYWAAYEWLPESGLRTLIAEQVGPPGSVARALQG
                     SNSCTVFGDGWQAYENDIRNAIEAVGGQVQEVSEETHRPSAVSVGLAGQRRLDGGQIA
                     EQELVPRYVQRTEAEVKFDEQQGVSALERRRQRVASKLTQRRARPVPKGSDVRRRT"
     gene            complement(9626..10486)
                     /locus_tag="PK589_12875"
     CDS             complement(9626..10486)
                     /locus_tag="PK589_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20546.1"
                     /translation="MATKTVRVNQVRQLLSILLLWVFTLPGCALFGPKDTTPLKEATA
                     EQLTALLKEQAGEVRTIKGLFSAKITGGILPIGQRVQGTVFYQRPNAIRLRGFSAVGS
                     EIFEFVQLEDQFKLRLPTMGREVTGRSSEADQLGQLTRPFQLSVWAMSGVTGTQAVGP
                     QEQVRLSEDGSRYRLDVLTAPGPSGAAPYISRRIWFDRRNLLVVQEERLTPAGEIEAT
                     MQFDDYRAVGPLPTAGLASNGQSPEAATRIMRPFTIRMTDGQGSGSLQVTFHELVPNE
                     PIKPGELGRV"
     gene            complement(10492..11853)
                     /gene="radA"
                     /locus_tag="PK589_12880"
     CDS             complement(10492..11853)
                     /gene="radA"
                     /locus_tag="PK589_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNJ20547.1"
                     /translation="MKAKTTFHCQACGHQAPRWLGRCPDCGGWNTLKEERLPAAPKGR
                     QAMPLTAMAVATPISDIEIVGEPRHSTGMGEFDRVLGGGVVPGSVMLIGGDPGIGKTT
                     LLLQALPLLAETGEQVLYVSGEESPRQIKMRGERLGIGGKHLLILGETSLEQILKAIQ
                     EIKPAAVVVDSIQTVYTEQLTSAPGSISQVQEVAGQLMWFAKRNNVPVFIIGHVTKEG
                     AIAGPRLLEHIVDTVLYFEGDKSHSFRILRAVKNRFGSTNEIGVFEMKDGGLEEVSNP
                     SELFLAERPQRSTGSVVVSSLEGTRPILVELQALVSGTNYPMPKRMANGVEPNRLSLL
                     LAVMEKRLGMHLSGQDVYVNVVGGIHIDEPAIDLGIVAAVTSSLRESPIDFTTLVMGE
                     VGLGGEVRAISQAEMRIREAAKMGFKRCLLPERNVAKLDPVDGIELVGIREVGDALDA
                     VLA"
     gene            12031..12492
                     /locus_tag="PK589_12885"
     CDS             12031..12492
                     /locus_tag="PK589_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20548.1"
                     /translation="MTDLIPPTTSRVADRTDADINARIRRQTEANVAYFAKGGAQAIS
                     ERLRELDREWDVERLLEANAATIALTGLCLGSLVNRRWFLLPAVVGGFLLQHAVQGWC
                     PPIPIFRRLGMRTQTEIEQERYALKAMRGDFANIPRDGDDRTAAVLNAVRA"
     gene            complement(12501..13310)
                     /locus_tag="PK589_12890"
     CDS             complement(12501..13310)
                     /locus_tag="PK589_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNJ20549.1"
                     /translation="MTFSSRTVSRNFLSHIRDVAKTKRTRDRERVFVLEGTKPILELV
                     RSAPRQIVCLVVAPTFLERQSPGDVQEIVRSGCTVHTCPEHQLAQLSDVETSSGALAI
                     VHQPTWDQAAMLAQPRVFGLYGDMLQDPANIGTIIRTAAGLDVSALWMAPHSVDVFNP
                     KVVRATAGTLFQLPIFSHTSFENLLSLGCTILAADVGAHSNSVPIRSIQSIPARTIVA
                     IGSESRGLSDAVLDAAAMRFTIPLKPGVESLNAATAAAIALFHLSGLPVES"
     gene            complement(13379..13720)
                     /locus_tag="PK589_12895"
     CDS             complement(13379..13720)
                     /locus_tag="PK589_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20550.1"
                     /translation="MLGTDIRGIMAEEEEVQRRQEALQSLMSMRERLLRESLEARIKR
                     ARGTGDWTNLSPAECASIYKEERVHLRAQLERLKAERDRTRGKLSALKRAKVRAQRIR
                     AAEAASGKKRK"
     gene            13897..14367
                     /locus_tag="PK589_12900"
     CDS             13897..14367
                     /locus_tag="PK589_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ20551.1"
                     /translation="MPHIGALVRAWRVSQKCSEQAFAQRAGITPNLLESLESEQLDPT
                     ASTLEAFAKALQIPLSWLFIHPTEFDLLCKDAEEEPAPLSTLTGADPVLDRLLLAAGH
                     DRTLFVLLTALMQSGDPKLLRAAEVSLRSLVKQSKQATVPWQSRPPGHFEPPSD"
     gene            complement(14394..16403)
                     /locus_tag="PK589_12905"
     CDS             complement(14394..16403)
                     /locus_tag="PK589_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HNJ20552.1"
                     /translation="MMHEPERLSEPVDAPLVPASVSLPEITPKAVLLSVLLAAVLAGA
                     NAYLGLFAGMTVSASIPAAVVSMAVLRLFRQSNILENNIVQTAASSGEALAAGVIFTI
                     PGLVLIGYWTSFDYWQTFTVSLVGGVLGVLFTIPLRRALIIHARLRFPEGVATAEVLK
                     IGATSGGNAGRRVRLLLSASALGGGLKFAEGGLKLWSESLEGALELGRMTFYGGLNLS
                     PALVAVGYIIGLNTALVVFLGGAIGWLVLLPLYQALVGTPADVTGVAAAKVVWSGQIR
                     YIGIGAMLVGGVWTLIQVRGPILHSLRQLLSLYAARRDDRGASAPLRTERDAGVVWLI
                     GLTVASLIPMVFLYRHLLDHSLFAGAGLTVLMVVTAFLFSAVAGYMAGLVGSSSNPVS
                     GVTIATIMLAALLLLAILGREHPAGPAAALLVGAVVCCAAAMGGDNLQDLKTGHLVGA
                     TPWKQQVMQVIGVATGAVVIVPVLSLLQAKYGIGVATAAHPHPLSAPQATLMANLAKG
                     VFGGSLPWHLVGIGMALGVVIIALDQRQAQRQAQLRFPVLAVALGMYLPLKLSAAIMF
                     GGLLAEVVRRGRPASETPTDDQGLLCAAGLVTGEAMVGILLAVPIALSSVWPSLAGDP
                     FQLFADPPLGGWPGLLALALVGLFLVRAAQAAGGAGNADATPRGV"
     gene            complement(16408..17592)
                     /locus_tag="PK589_12910"
     CDS             complement(16408..17592)
                     /locus_tag="PK589_12910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNJ20553.1"
                     /translation="MTSSTARVRLTAERAGPRYYGHLWVFDNNVAEVLGTPAAGDLVD
                     VYTHQKRFFGRGLFNPHSKIRIRLLTFHEEPIDEAFFAARLRAAAALRRTVTPHANAC
                     RLVHGESDLLPGLVVDRFADVAVMQALGYGMDLRKDLLGDLLVQEAGVKSVYLRNDAK
                     SRTLEGLPLSKGFLRGEGPTTVPIHEGKAQFTVDIAEGQKTGWFCDQRENRLAAAAYA
                     KGKRVLEAFCHTGAFGVQAALAGAQSVEGLDVSAAAVALAQAHAAQNHVASICTYRQA
                     DAFDELRVLERNQQRYDLVILDPPAFARSKKALPHALAGYKDVNLRGLRLLQSGGVLV
                     SCSCSQPVSDDDFWMMLQSAARDANRQIRLLEQRGQGPDHPVLAGMPETRYLKCFIVQ
                     VF"
     gene            17804..19456
                     /locus_tag="PK589_12915"
     CDS             17804..19456
                     /locus_tag="PK589_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNJ20554.1"
                     /translation="MTTHTIGTAVLDRLHRLGVRHMFGIPGDYVLSLYKLMESSPIQH
                     VATTREDCAGFAADAYARINGIGALCVTYCVGGLNTVNAIACAYAERSPVVLLTGSPG
                     LSERARNPYLHHMVREFSTQREVFEKMTVAAVSLDDPLTAEREMDRAFAALLRYRRPI
                     YLEIPRDMVHVPLAYTSHKTSILDEPTDKAALSEAVTEVRVMLESAKRPVILAGAEVG
                     RFGLHDELTKLVERLNVPIASTLLGKSIIREDHPLYVGVYSGLVARDEVKEFVNQTDC
                     LLILGSILSDVEDLDARSALFSDGHTIHATADRVAIKHHRYDSIRFEDFVKALANAPL
                     PSFPKPQLPAPRAPEEPMPPADASVSLHGVFRHLDTVLQEKTLVIADVGESLFASVDL
                     HVHRRFEFLSPAYYTSMGFAVPAAVGASFADPSLRPIVLVGDGAFQMTGSELSTAVRY
                     RQAPVVIVLNNHGYSTEREILEGPFNDIHEWRYERICDLIGGGQGSRVATHGEFVDTL
                     AKALADPSQPYVINVLLDPSDRSPAMMRLARRLAKRLSTDRP"
     gene            19646..20608
                     /locus_tag="PK589_12920"
     CDS             19646..20608
                     /locus_tag="PK589_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20555.1"
                     /translation="MSPKMPDGKTMRDGQPFSARLNNVVTTEREFDELVTKSLPELLD
                     RATGQTKRFLRETNQWGDDITHEKLALRWGYELVERFVVFGRTEVPCRPFFLLDSLIA
                     KSFSQPDPLCYHKELLTPVGRFLDGLASRAVVSRDALIALFYHFYGFSQAHVVKLLGF
                     GQAESQRVYKNFERWRQSGWQRTMNETGLSGPEIEILEQELRTRPDHLNNEVDRLMPV
                     LQSHYRKSEPEHYPCLSEQKWGQLFHDDYGHDYRVWHLAFCRDCFLEVADRRQADVSS
                     GVKPQVNLHIHPLKKGGVLALFGGDRGGRHHGNTRAQRLSRTSA"
     gene            20565..21866
                     /locus_tag="PK589_12925"
     CDS             20565..21866
                     /locus_tag="PK589_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20556.1"
                     /translation="METPELNDYRAHLLDALDDQPRRAAEERPGFLQVLVNHERIGTL
                     NCSQTDPSFAYTARERSIQHLELRSESGVLIGGCVAPEAGQKSVRIPVGKGSLTLHVQ
                     NHFDGGSLNVQYEVAPGWWSHLATAIGLPAGAAVASTQGSPVWSMTTAFAQVVLAVGV
                     VALLAERIPAWVGSEDRAHQLEEELAARQSTQDQIDHVHQQLAQLVESQAAAVAVSRA
                     EQDRIAQLNTLIDTVAHTQQRLSTQVVAVQEDLKTVKTDVAQEVQTGMRVAVTAVEAD
                     RELVRQDLQAVKSVNETLVKQVALLESRNRELHARLALTSLEVAKANAASKPVVVAKN
                     EPPKETPAPTAVAVSDNLRDADRQAFMFWVAFQDGTTEKSIEDLVQELNGIKKGPMKS
                     GWYSVEVNLPNPEPPDRFLESVKRAKIVKSVVTSKAMPPAQ"
     gene            21998..23230
                     /locus_tag="PK589_12930"
     CDS             21998..23230
                     /locus_tag="PK589_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNJ20557.1"
                     /translation="MSDFHQNGLVTVLHRLGGSNLEQLEKELERHAASNPIALVLPSL
                     YSELENPALKHIVQVLKDIRYVNEIVISLDRASALEFRLAKQFFSVLPQRVRIVWNDG
                     AGIQDILKLLAQAELDTGLQGKGRGCWMAFGYVLARGESNVIALHDCDILSYNREYLA
                     RLCYPIVNTNLGYEFCKGYYSRVTNRLHGRVTRLYFTPLIRSLQQVAGAHPLLTFLDS
                     FRYPLAGEFAMVRDLAWINRVPGDWGLEVGVLSEIYRNCALRRICQADIADAYEHKHQ
                     GLSADNAEQGLQKMCVDITKSLFRNLASEGVVLSEGVLKTLRATYLQSAQEAITRYQN
                     DAAINSLQFDRHAERMAVEVFLKGMKIATEAFLEDPLGVPMISNWSRVTGAIPDVFER
                     LIGAVERDHDWDPVGDRV"
     gene            complement(23362..25497)
                     /gene="glgX"
                     /locus_tag="PK589_12935"
     CDS             complement(23362..25497)
                     /gene="glgX"
                     /locus_tag="PK589_12935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNJ20558.1"
                     /translation="MTEVLEGRPTPRGATWDGQGTNFSLFSAHATKVELCLFDPTGEK
                     EIDRVSLPEYTDQIWHGYLAGIHPGTPYGYRVHGPYDPDAGHRFNPNKLLLDPYAYAH
                     MGELQWNPALFGYQMESGDDRTFDERDSAPFMPKCTVVDQNFDWSGPRDRRTIPWDDT
                     VVYEMHVRGFTKLHPAVPSTLQGTYEGLATKEVLDYLQSLGVTSIELLPIHTFVNDSH
                     LLEKGLTNYWGYNSIGFFAPDPRYAANRPESVRECKEMIARLHDAGFEVILDVVYNHT
                     AEGNELGPTLSFKGIDNSSYYRLQPEQPRYYINDTGTGNTLNLSHPRVIQLVTDSLRY
                     AVQQMHVDGFRFDLGTILAREPNGFDNRSGFLKVCSQDPLLATVKLIAEPWDCGPGGY
                     QVGGFPPGWAEWNDKFRDDVRDFWRGELAATNLADRLCASASVFNHQGRRPWSCVNFV
                     TAHDGFTLHDVVAFNEKHNEANGEDNQDGSSNNRSWNCGAEGPTEDASINALRDRQMR
                     NLMATLLISQGTPMVLAGDEFGRTQRGNNNAYCQDNEISWVDWSLLETRSSLFQFVKE
                     LIAFRHHYPILRRNLFLTGQLAEDSGVRDVTWINANGGPMEEHHWTDTTIHCFGMLLD
                     GRAPTTGRRQPGKDVTVLVLINGHHEPVQFTLPPCVGASEWTLLLDTNLPDRPQNQTF
                     QMGTQYLLTDRSLVLLVLGAKRTEEIIPV"
     gene            complement(25636..25803)
                     /locus_tag="PK589_12940"
     CDS             complement(25636..25803)
                     /locus_tag="PK589_12940"
                     /inference="COORDINATES: protein motif:HMM:NF023180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HNJ20559.1"
                     /translation="MPNIVLVILILLLVGVLPTWPYSSGWGYYPSGGLGLLLLIVLVI
                     ALSGRRGALRS"
     gene            complement(25910..27976)
                     /locus_tag="PK589_12945"
     CDS             complement(25910..27976)
                     /locus_tag="PK589_12945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ20560.1"
                     /translation="MVRTRLEERIIILAPIGQDAPAMARVLISQGVDAIVCATALDTC
                     RELAVGGAVLLVTEESLELPHISQLLHHLKAQPPWSELPMIVLTRGGESRLARLLDLV
                     AEAAGSITLLERPIGEATLMRSIEVGLRSRRRQYQVRDLLEQETLSQQQLRESEQQYR
                     TLFESIDQGFCTIEVLFDEQQEPVDYRFLLVNPAFAVQTGLRDVVGRHMRDIVPQHEE
                     HWFRIYGNIATTGEPLRFEKEAKALDRHYEIYAWRIGLPSEQKVAVLFNDITQRKASQ
                     FRLEQFAEELERKVSERTYELVASQQQLRALATELNLAEQRERTRLAAELHDHLAQML
                     VLVRLRLGQAKVGPLSHSIQMIKQAEDVVDESLTYTRNLVAELSPPVLHEFGLFVALR
                     WLGEQMRRYHLEVSVRIDTPEELKLPENQAVLVFQSVRELLLNAVKHAGSKKAWIAAA
                     SEDGVLSIVVEDRGVGFDPITLSSQKLAAMSSKFGLFSIRQRMEALGGRLELRSKLGE
                     GTKATLVLPITQLSEQATGISLSYIAPSAHVARAPSTGHCKSVPYRVLVADDHAMVRQ
                     GLCLMLDACPDIEVIAEAANGQEALELARRLKPSVVVIDINMPLMNGIEATAQIRTAL
                     PDTIVIGMSVNASVDNQEAMRTAGASVLLPKEAAGDQLYGAIQSAMKGLSEHTGPLIR
                     SQETRL"
     gene            complement(27963..29477)
                     /gene="gvpD"
                     /locus_tag="PK589_12950"
     CDS             complement(27963..29477)
                     /gene="gvpD"
                     /locus_tag="PK589_12950"
                     /inference="COORDINATES: protein motif:HMM:NF018756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpD"
                     /protein_id="HNJ20561.1"
                     /translation="MTDPTRQADSRCETGIDGLNEILGDGLPANCMYLVQGDPGSGKT
                     TLALQFLLEGVRLGEKTLYITLSETKEELVTVARSHGWSLDSISLFELSAIDSLVRPE
                     ARTTVFHPSEVELTKVSEMLQSEIRRIQPARVVFDSLSEFRLMAETALRYRRQLINLK
                     QELAKYNCTALLLDDRMDNSRIGGDPHVLSLTHGVIEMEQLSPDYGRSRRRLRVLKMR
                     GMKFREGYHDYSIEKGGLRVFPRILASDHRADFSPQAVSSGNQEFDELLGGGLDRGTT
                     TLIMGPAGTGKSTLAMQYAAQMAQRNESSVLFAFDEIRGIVLARATAVGLDVERHINT
                     GLIDVQQVDPAELSPGEFGMRIREAVEGGCTLLIIDSLNGYLNAMPGEKYLVNQLHEM
                     SAYLNQKGVVTILILAQYGLIGQAEAPLNLSYLADTVVSVRFFEAAGAVRQALSVIKK
                     RSGGHEKTIREYQLVSDGGLRIGPPLKQFQGVLTGTPSFYGALEQVMERPDGAN"
     gene            29882..30637
                     /locus_tag="PK589_12955"
     CDS             29882..30637
                     /locus_tag="PK589_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20562.1"
                     /translation="MPNWLAYAGAILTGLIPPLIMYLIGSQKKVLGYALTQNEPMINL
                     RHPDLDGRLSMSLDGKNLNGCRIMGLVIQNLGMKDIEEQSVHLTFPAGVTVLHAHCSS
                     SALAIGPKIEARQADSHEVRIPLMNPGDKLMVRLLMTGNEAGRILVTAKGPSLKFKPF
                     DPTTFISPAVNRGLLVMSGAIFAWIVWACAGTDRFMARPFWDKTITLALIGLFLAIPS
                     AILAYQGTIRLLKRALQWREGNRASSLQPHPEL"
BASE COUNT         6618 a         9491 c         8569 g         6151 t
ORIGIN      
        1 atcgtgtatc tgcccatcat gttttttacc ggcatcatca agtacctctt cgtgccgctg
       61 gcgatgaccg tcgcctttgc catgctggca gactatgtcg tgtccatgtc ggtcaccccg
      121 gtagtgctcg cctggctcta ccagggagga catggcaaag gcacggaaca tgaggcctcg
      181 gccgatgaag gatggttccg ctatgtgctc gccatctatg agccgatctt gaagggcggc
      241 ctgcgcttca agccggtcgt gatcggcctg gcgacggtcg cgttgatcgc caccggcgcc
      301 ttcttgattc cccgcttgca caccgaattc ttcccaaaag tcgacgcagg caacttcacg
      361 atgctggtca ccgcgccgga aggttcgcgg atcgaaaaga cgacggccat cgtcggacag
      421 gtcgaacaat tggtgcatga gacgatcccc aaagcggatc tggaagaagt gatttccaac
      481 accggtctct attatggcga tgccgctcga ttcgccccga acacgggcaa ccataccgcc
      541 ttcgtgctcg tcaacctcat cacaggacac gaaggccgca ccgaggacta tattgcccag
      601 ctccggagca ggctcaagac ctcgctgccc ggtgtcgaga tctctttcca gaccggcggc
      661 atcatcagcg atgtgttgaa tttcggattg aaagccccga tcgacattca agtgaaaggt
      721 ccgagtctcg acgtgatccg tccggtggcc gagcggattc aacagcagat cgcccaggtg
      781 cccaataccg tcgatgtgcg aatcaagcag ggcaagagtt acccggaatt gcatatcgat
      841 gtggatcgca ccaaggccgc ctattacggt atcaatcaga accgcgtcgt cgtggacgtc
      901 atcaccggca tcagttcgaa cctggccctt tcgcccaact actggctcga tcccaaaacc
      961 gccaacgggt atttcctgct ggcgcagtat cccgagcaat cactgacgac gaccgaagat
     1021 ttgctcaaca ttccgatcat cggcgcgcgc acccctctcc tccctacctc cagtctcacg
     1081 ggaggcggca ttcagggatc cacgctcgcc ctccaaaata ccccgtttgc cggaaggcag
     1141 atggaaatcg cgagcggcta ctacgcatcc agcgacgacc gacgcgggcc gcccgtcatg
     1201 cttcgcgatg tggctcaact gaagttcaag accggtcccg attcggtcga ccactacgat
     1261 ctgtcgcggt tgatcaatgt gctcgtcacc cccgtcggca acgacctcgg ccatgtcgcg
     1321 aaggatatcg agcaagtgtt ggcgggcatt cagttgccga aggacgtgac cgtgcagctg
     1381 cggggcgaag tggccaacat gcgcggggcg attcaaaact ttgccctggc gctcccgctc
     1441 gccgtggtgt tgatttatct ggtcatggtc gccttgttcc gctcctttat cgatccgctg
     1501 atcatcctgg tggccgtgcc gcttgggtgg atcggcacgg tgttaaccct gcacgtgacc
     1561 aacacgtcgg tcaacgtgga atccatgatc ggcacgctga tgatgatggg catcgtggtg
     1621 tcgaacagta ttctgctcgt cgatttcgcc aatcgcatgg tgcgcaacgg ctcgaccgct
     1681 gcgcatgccg tactggaagc cgggcggcgg cgcatccggc cgatcctcat gacggcgctc
     1741 gccacaatcc tgggtctgtt gcccctggcg ctcgggttcg gcgagggcaa cgaaaccatg
     1801 gtgccgttgg cccgcgcggt ggtcggcggg ttggctgtca gtacgatcat gacgctgctg
     1861 gtcgtaccgg tgatgcacag tctggtattg attcgtcggg aacgcgcgcc gctctctgcc
     1921 acggctcccg cgacctcgga ggacatttaa tggacgaacc gtcaacgact ctccgccccg
     1981 tcagggccac gcgccggaaa atccgctgga tcgccctcgc catcgtgctg acacttgtca
     2041 tcggcggact tctgtattgg cgagagatcc atgacgtggg ccatccggtt ccccctgcgt
     2101 ctcccgcagt gcccaccccg ccacaaccgc ccgaggaatc cgtggcggta ccggacagcg
     2161 ctcccgtgga cgtgcaggtg accaagccaa ccagacgcga tttggtctat acggtcaaac
     2221 tgcccgcgaa tgtgtctcca ctctatcaaa ccacgctcta cgccaaagtg tccggttatc
     2281 tcaaatggat tggcccggac aagggcgatg ccgtgaagcg gaatgatgtc gtcgcggtca
     2341 tcgacgcgcc ggaagtggaa gagcagtacc agcaggcggt gtcggattac aaaattaaga
     2401 agctgaccta cgaacggctg gcgaaagtct ggaaagagtc gccggacgtc atcgcgaaac
     2461 aggacgtgga cgtggccgaa gcggcttatc agggagcaag gcacctgatg gaacagcgcg
     2521 tcgtcatgcg ggattacacc aaagtccgcg ccccttacga cggcatcgtg acggcccggt
     2581 tcgcggaccc cggagccttg attcaaatcg ccacgtcctc ggccaccagc gcgattcccc
     2641 tctttacgat catggatctg aacaccgtgc gcatttatgc caatgtgccc caggacgaca
     2701 gcccctggat cgtgccggga aagacccagg ccacggtgaa ggtgaccgaa ctgccgtccc
     2761 gctcgttcac cggtctcgtc acccgctcca ccatggcgct cgatccggcc acgcgtagtc
     2821 tgctggtcga aatcgattta cctaaccagg accacgcact ccgtcccggc accttcgccg
     2881 aagtcaccct cggtctgcgt gaaattccgc aggcgctcgt ggttcccccc caggctatca
     2941 ccagcacgcc gaagggcaag tccgtcttcg tcgtcgaaga tggcaaggcg aaaccggtgg
     3001 cggtacaaac cggcatcagc gacggccggt ggatcgagat cagctccggc ttgcgagggg
     3061 acgaagatgt ggtggccgtt ggcaaacgtc gtctgctcga agggatgccg gtcgtcgcct
     3121 cgccattcaa tctgccggac gccaaacttt cccaacagaa atttgaacgg cgcgcgccag
     3181 gcggcactcc tccaccgccg accgccgcgc cccctacaaa ccgagcggcc cagacacagg
     3241 gagatcgccc atgacttatt tactgctacg cagcacctct ctcatgctgg ccgccctgtc
     3301 cctggcggca gcatcagcct ccggtaccta tgccgccgag gcagaacagc cggtgccatt
     3361 gtcacgcggc agtttcctcg gcgtgcagca agcggtggaa attgccgtca agaaccatcc
     3421 gatgctgatc gagggccagg ccaacttgaa ggcttcggag gcgcgtacgg aacaggcacg
     3481 ctccctgtac tatccccagg tctacgccaa cgccaacacc gtcgccggtg ccggcgtgag
     3541 caacccacgg ttcctgatcg gaggaggatt gttgcgcgag aaccaaacca cctttaccgg
     3601 aggcgtgatc gccaaccaac gcctctacga tttcggctat accagcaacc tcatcgagtc
     3661 gaacaaactc gccgaacgcg cgcaggggca ggatgtcaat gcaagacgtg cgctggtgtt
     3721 gctgtatgtg cagcgcgcgt acctgaatag tctgaagcgg catcgtctgg tgcagatcgc
     3781 ggaagaaacg gttcgcgagc gtggcatcat cgccggccag atcgaaacgc tttatcgcca
     3841 gcaactgaaa tccaaattgg acttcgacct ggcccgcgtc gaactcgtga atgcccaatc
     3901 cctgctggtg cggagccgca acgacctcaa agccagtttc gccgacctca accgcacgat
     3961 gggcatcgcc ggcgccgatg actatgtcct tgaggatatt tccgttgccg ttcgcccgca
     4021 gaaaacgctg gagaccctga tcaacgaaag cctctctcat ccggaagtca aacgggcgaa
     4081 agagcagacg gcgtccgccg atgcgcgcct caccgcgacc aaacgccaat acctcccgac
     4141 cgtgtcggcg atcgccagcg gcggcacctt cgatccattc gatccgcgcc agaaccagca
     4201 gaccggcggc tggtggatgg ccggcgccat ggtctcgatg ccgctgttca ccggcttcct
     4261 gatcgaaaac caggtcgtgg aagccaacgc caaccggcag gccgcttccg ctgcaaccag
     4321 taacatcgag caggcgctga cgcagcaagt gacgaatgcc tacctcgaca ctctcacctt
     4381 cgcgcaacag atcacactgg cggaagaaca ggtgaagacg gcacaagagg cgttgcagtt
     4441 gtccaaacag cggtacaaac tcgggttggg cacggtcgtg gaagtgacgc aatcagaagt
     4501 ggcagtcaca gcggcgcaaa cgcgactcgc ggacacgcag tacgattata agatcgcaga
     4561 agttacgctg gcctatactt cccaaggcga cgataccggc cggattcttc tgatgagccg
     4621 cgcatcgacg gcttcccgcc ctggtgatgc agggagtcag gcggaaggac cacccgcagc
     4681 ggcatactag ggcgtcgtga ggcttccaca gactgcagga accgtttcag gagcgacggg
     4741 cggttcagat attctgaccg ggaaagacaa tcgcgagatc gatcaccagt tcgtcttttg
     4801 tattggcgag gagcccatcc cagatgctgg tcgtttgagt gactaacgcc tggtcgaggt
     4861 cgaaaaacag atcgaatcct ttggagatga tgggattcat cgggtaccgt cgatcgtaaa
     4921 tcatcccgta agccggaatg ccatacagcg tctgccaatt ccccaaaata aagtgcagtt
     4981 cggtaccccg gatatacatc cctccagagg tgatcgtccg cttcaccgac gtttgaggct
     5041 ggctgaggta gtaggtcact cgctcatttt ctcgtgctaa ccgtagggcc tcgaccagtt
     5101 gaggcacgag aatcatgatc tccgcatcgc ggaacatggg caatcgtcga gagtcatcgc
     5161 cgatccagcg ctggaccatc gtccggtgtt cctgaaccgt gagccccatc agcacccgcc
     5221 tgacctgctc ctgagaaaac cgggccggat gcgtaaaatg cccgggcggc cactccggca
     5281 gcacattggc atccaattcc aaccggacga aattgacggg gtcttcgtag acggtccgcg
     5341 acgggacctg aggaatggcg cagcccgcag cggtgaacac cgctgcggcc aacaccgcga
     5401 cctgtgaaag gtgaaccgtg acgcgtagga accgaagcac cggggtcatg acgcagtcgt
     5461 ctccttacgc ttcaacggtg cgtcgcatta ttcggtaatc gtcacggtca tttccacgcg
     5521 ctccagcggg ttgtcccgtc cgtcacgctt ggctccgacc actttgtcga cgacgtcgag
     5581 cccgctctgc acttccccga acaccgtata ttgccaatcc aagaaattcg cgtcgttcac
     5641 gcagatgaag aactgcgaac ccgcgctgtc cggatcattg gcgcgcgcca tcgacagcgt
     5701 tccccgctta tgcggcttgc tgttgaattc ggccttcaca ttgtggccgg gtccacccat
     5761 cccgtgcgag gcgcgatccg gattcttgct gttgggatcg ccgccttgaa tcatgaagcc
     5821 tggaatgacc cgatgaaagg tggtgccgtt gtaaaaccct ttcttcgcca ggtccgtaaa
     5881 gttcttcaca tgattcggcg ctacgtcggg gaaaaagcgc agcacgatct gcccccacgg
     5941 ctcgttcttg gatgtcacag taatggtcgc ttgtggtgaa ccggtttgtg ccgtcatctc
     6001 gtgtcctttc ctactcagtt aaacttgaag ggcgcgacgc tctcgccttc taacccttgg
     6061 ctgactgccg tccaggtgag gccgtcgtca tcgctgcgaa acacgccgga actggtcccg
     6121 gcataaaaac cgcccttatc gccctcgatc aacacttgta ccgatagatt gctgaggccc
     6181 ttatttaacg gcatccattg ccgcccctta tcgacggtct tgaaaatccc ccgtcccgtg
     6241 gcaacataca cgccgcgctc attgctgatc aaaccgcgga tggagtcatt cgggagggca
     6301 cggctgatcg gcaaccaggt tttgccacca tcgccgctgc ggaacactcc accgtcgaat
     6361 gtcccggcgt acaccccttg ctccttatcg acggccagca cgcggataaa attctctacc
     6421 aagccctcgt gatctttgag gccctgtcgc aggcgcgtcc atcccggcgt gcgttcttta
     6481 taactatgca cccctttgcc tgaggttccg gcatatagcg tgccatcatc agtcctcgcc
     6541 agcgcatgaa ccagaatctc atccaggccg gtggtcacga ctttccagtg atcctgcgcc
     6601 gcattcaaca ggtagacacc gtcgccggtt cctgcataca gtcccttggc gtccatcatc
     6661 aggactttga tctccaaccg tttcaagccc ttgttgatcg gctgccaggt tttcccggca
     6721 tcgcgggagc ggaacacgcc gccacgaaat gtccccgcat agatcgcgcc gtctttggcg
     6781 gtggtgaggc agagcacgaa gggatcggtc agtccctcat tggccgacat ccaggtgccg
     6841 ccccgatcgg tactccggaa aatgcccatg ccgaatgagc ctgcgtagat cacgcctccg
     6901 gcacctgacg cgagggcttg aatactcgtg ttgccgatat cggctggtga ggaaggatcc
     6961 tgcccgggat gtccctgttc taacttcagc gcgcctcctc cctcattccc gattagcgag
     7021 ggagcggcat gcactaatcc ctgtaaaccg ccgaccgtca acgccgtcaa caccaacatg
     7081 tgacgcagca taggagatca cccttcaaaa attgtgaacg ccggcctatc ggacgcgatg
     7141 cccggcggta ccgatgggta tatccgggtc gaggggcaaa ttcatgtcgg gcgccggtcc
     7201 tgacgaccgc ccgaagatct tcgcgccgat gatcccgatc tcatagagta ccatgagcgg
     7261 gaccgccatc agcaacatgg tgaacagggt ggcatccggc gtcacgatgg ccgaaatgat
     7321 caaggccgcc atgatggcat gcttacggta atgggccaat accgccgccg agaccacgcc
     7381 gacccgggac aaaatggtca gcaccagtgg cagttcaaat gcacaaccga agatcaggag
     7441 gaatttgacg ttgaaatcga tataggtgcc gacaccgagc gcgggcgtga tgtcgcgatc
     7501 cataccgaag ctgacaaaaa agtcgatgac cagcggcagg atgacgagat tacaaaagat
     7561 gaggccgagc gcgaacagga gcgccgcgag caggaaaatc ggaattgccc agcgctgctc
     7621 ctttggaagc agcgctggtt cgatgaattt ccagaattgg taaaacacga tcggcaggct
     7681 gagaatgatg ccagccagaa acgacacttt gatggaggca aacaacgctt cggtcggtcc
     7741 gtagaagacc agctggttgg ggaacggacg gttgagccac gcgaccatct ccgttgaata
     7801 ggcaaacgcc gacgcaaacg cgacccccaa ggtgaccgcg atgatcagca accgcttttt
     7861 cagcgactga atatgggcgg ccagtggtgc gagcatgtcc ggtctgaacc aatgcctccg
     7921 cgctcggtct ctccgctctg ccccatccgt ccgcgagaag agcccggcgc agaactccta
     7981 tcgatcttcg ttaccgtgag gatcgaatga gttcggcaat cttatccttc gtggccagtt
     8041 tttctttctg cagctgtttc ttcagcactt cttcggccgg cgtcagcacg tgccgccgtt
     8101 gtagatcggc taatttctca tcgaggcgat ggtgagattc ttccagtgcg cggaattctg
     8161 tgttggatcg ccgtaaccgc tcagtaatcg ccgtctccgt ctgcatgtcg cacctcctgg
     8221 ttgactacat acgttcgtgg atgaccatcg cgttacccgg ctcccggagc tcacactccg
     8281 ggctgccgac acacctcact cacctgcagc ggcgtcattt ccatcagcac cgcatcttcc
     8341 tcgggatgat tgtaataccg tgatcgtttg gcggtctgac gaaaccctag ccggcgataa
     8401 agcgtctgtg cctcgagatt cgattcccgc acttccagca tcgccctgga ggcaccacgt
     8461 tccactccag tttgcagggc ggtgcagacc aacttcgttg cgacgccttg ccgccgaaac
     8521 ggttccaaga ccgccaggtt catcagccgc acttcctcaa acacgaccca aaaacaaaaa
     8581 tacccagcca ccgcaaagga tcccgacaag gggtcggggc acttcgcgat caaaaagttc
     8641 gcaaattggt tgccggagag ttcggctgcc aacattttgc gcgtccaggg ggctgagaag
     8701 caagcctgtt caatggcaag cacctcatcg aggacggctg gtgtcgcagg ttcaatcaag
     8761 atcgacgtcg atgtcatctc agagaacagg ctccatgtgg cacactacgt cctgcgtcgg
     8821 acatcactgc cttttggaac aggtcgagct cgtcgctgcg tcagcttact ggccactcgc
     8881 tggcgtcgcc gctcaagcgc ggagacgccc tgctgttcgt caaatttcac ttccgcttcc
     8941 gtgcgttgaa catagcgggg aaccaattct tgttccgcaa tctgtccccc gtccagacgg
     9001 cgttggcccg ccaaccccac actcacggcc gaaggacggt gtgtttcctc gctgacttcc
     9061 tgcacttgcc cgccgacggc ctcgatagca ttcctgatat cgttctcata cgcctgccat
     9121 ccatcaccga acactgtgca ggaattgctc ccctgtaagg ctcgagctac cgaacccggc
     9181 ggcccaacct gctcggcgat gagggtccgt aggcccgatt ccggtaacca ctcataggca
     9241 gcccaataga cttcgttgtg ccggcttttc aggaccggca ccaagagccc tttcgcgtca
     9301 cgcaggttcc aggccatggc ttccaatgtg gggaccggtg caatcggaag acccaatacc
     9361 gcacgaaacc ccaacatggt cgcgagccca acccgcaaac cggtgaatga acctgggccg
     9421 atggataccg ccagactctg aagttgcttc agtgtaatgc tcgtctcacg caacacacga
     9481 tcaatggcgc ccagtaacga ccgtgcgtgc gatccgtcgg catcttgctc aacgagggcc
     9541 agcaccttct caccgctcaa cagggccacg ctctgccagg ccgtcgcggt atcgattgct
     9601 aacaggtggt gatccggttg caccttcata cgcgtcccag ctcacccggt ttaatcggct
     9661 cattcggaac cagttcatgg aatgtcacct gcagacttcc ggatccctgc ccatcggtca
     9721 tgcgaatggt aaagggacgc atgatacgcg tggcggcctc tggcgattgt ccattggagg
     9781 ccaagccggc cgtcggcaac gggccgactg cccgatagtc atcgaattgc atcgtcgctt
     9841 cgatctcgcc ggcgggtgtc agccgttctt cctgcaccac cagcaaattc cgacggtcaa
     9901 accagattcg tcgacttatg taaggagccg ccccagaggg tcccggtgcc gtcaacacat
     9961 ccaggcggta tcgagagccg tcctccgaca accgaacctg ctcctgcggg ccgacggctt
    10021 gcgtccccgt cacgccactc atggcccaga cactcaactg aaacggacgc gtaagctgtc
    10081 ccagttgatc ggcttcagaa gatcgaccgg tcacttcacg gcccatcgtc ggcagccgca
    10141 gcttgaattg atcttccagt tggacgaact cgaaaatttc gctacccacc gccgagaagc
    10201 cacgaagcct gatcgcgttg ggccgctgat aaaacaccgt gccctggact cgttggccga
    10261 tgggtaagat gccgccggta atcttggcac tgaagaggcc tttgatggtt cgcacttctc
    10321 ccgcctgctc ttttaagagc gcggtcaact gctccgccgt cgcctctttg agcggagtgg
    10381 tgtcttttgg accaaagagc gcgcagccgg ggagcgtgaa gacccagagc aggaggatac
    10441 tcagtaattg gcgcacttgg tttacgcgta cggttttcgt agccacggtc ctcatgccaa
    10501 caccgcatcc agcgcgtctc cgacctcacg gatgccgacc agttcaatcc catctaccgg
    10561 atcgagtttc gccacgttcc gctcaggcaa caggcaacgc ttgaaaccca tcttcgccgc
    10621 ctcgcgaatc cgcatctcgg cctggctgat tgcccggacc tcgccgccca agcccacttc
    10681 gcccatgacc agagtcgtga aatcaatcgg gctctcgcgc aaactcgacg tcacggcggc
    10741 cacaataccc aggtcgatgg ccggctcatc gatatgaatt ccgccgacta cattcacgta
    10801 cacatcctgt cccgagaggt gcatgcccaa ccgtttttcc atcacggcca gcagcaagga
    10861 caaccgattt ggttccactc cgttcgccat ccgcttgggc atcggataat tcgttcccga
    10921 taccagcgcc tgaagttcca caagaattgg tcgtgtcccc tcgaggctgg acaccacgac
    10981 ggacccggtg ctgcgttgcg ggcgttcggc gagaaacaac tccgaggggt tgctgacttc
    11041 ctccagcccg ccatccttca tctcgaatac gccgatttca ttcgtcgacc cgaaccgatt
    11101 tttcaccgcg cgcaaaattc taaaactgtg gctcttgtcg ccttcgaagt acagcaccgt
    11161 atcgacgata tgttccagca agcgtggtcc ggcaatggcg ccttctttcg tgacatgtcc
    11221 gatgataaac acggggacat tgttgcgctt cgcgaaccac atcaactgcc cggcaacttc
    11281 ctgcacctga ctgatactcc ccggcgccga agtcaattgc tcggtgtaga ctgtctgaat
    11341 cgaatccacg acgaccgctg ccggcttgat ttcttgtatc gctttgagga tttgttcgag
    11401 ggatgtctct ccgagaatga ggagatgctt gccgccgatg ccgaggcgtt cccctcgcat
    11461 cttgatctgc cggggagact cctcaccgga gacatataag acctgctcgc ctgtctccgc
    11521 cagcaggggc aaggcctgca gcaataacgt cgtcttgcca atacccgggt cgccgccgat
    11581 caacatcacg gacccgggca cgacgccgcc gccgaggacc cgatcgaatt cgcccattcc
    11641 ggtgctatgc cgcggctcgc cgacgatttc gatgtcagag atgggtgtgg caaccgccat
    11701 ggccgtaagc ggcatggcct ggcgaccttt gggagcggca ggcaggcgct cctctttgag
    11761 cgtgttccag cccccgcagt cggggcagcg tccgagccag cgcggggctt ggtggccaca
    11821 tgcctgacaa tgaaatgtcg tcttcgcctt cattgatgtt cgcctcaggc tcagcacctg
    11881 tgtaccggca tcgtaataga aacggacgag aaaaaggaag gagatctgga gacgatataa
    11941 ggcctccgcg gggaatgcct agtccacatc gttgccctct gaatctcata gtggatgacg
    12001 tgccgaacac agaaacaaag gaggctctcg atgaccgatc tgattccgcc gacgacgtca
    12061 cgggtggctg accggaccga tgcggacatc aatgcgcgca ttcgccgcca aacagaagcc
    12121 aatgttgcct atttcgcaaa gggtggagca caggccatca gtgaacgcct acgcgaactg
    12181 gaccgcgaat gggacgtgga acggctgctg gaggccaacg cggcgacgat cgccttgacc
    12241 ggcctctgtc tcggaagtct ggtgaaccgg cgctggtttc tcctcccggc cgtcgtcgga
    12301 ggatttttac tccaacatgc cgttcaagga tggtgcccac caattccgat ctttcgtcga
    12361 ttgggaatgc ggactcagac ggaaatcgag caggaacgat atgccctgaa agcgatgcgg
    12421 ggagattttg cgaacatccc acgcgacggc gatgaccgga ccgcagcggt cttgaacgcc
    12481 gtgcgcgcct gatgagcggc ctatgattct accggtaatc ccgacaaatg aaacagggcg
    12541 atggcggccg ccgtggcggc attgagtgat tcgacccctg gtttgagcgg gatcgtaaat
    12601 cgcatcgcgg cggcgtcaag gacggcatcc gacaagccac gactttcact gccgatcgcc
    12661 acgatggtgc gagcgggaat ggactgaatc gaccggatcg ggacgctgtt cgaatgggcc
    12721 ccgacatcgg ctgccagaat cgtacatcct aaagacagca ggttctcaaa gctggtatga
    12781 gagaaaatcg ggagttgaaa cagcgtgccg gccgtggccc gcacaacctt tggattgaac
    12841 acatcgacag aatggggtgc catccataag gcgctcacat cgagtcccgc tgctgtccgg
    12901 ataatcgttc cgatattcgc cggatcctgc aacatgtctc cgtagagacc gaacactctg
    12961 ggctgcgcca gcatggcagc ctgatcccag gtcggttgat gcacaatggc gagagcaccg
    13021 ctcgaggttt cgacatcaga caactgggcc agctgatgtt ccgggcacgt gtgcacggtg
    13081 cacccgctgc ggacaatctc ctgcacatca cccggtgact ggcgttcgag aaacgtcgga
    13141 gcgacaacga gacagacgat ctggcgaggg gccgatcgca ctaattcaag aatcggcttt
    13201 gtgccttcga gcacaaagac gcgttcgcgg tcgcgcgtac gcttcgtctt agcgacatcc
    13261 ctgatatggg agagaaagtt gcgagagacg gtgcgagatg agaaggtcaa cagatacctg
    13321 gaggtctgcg gcactgcggg gaggagtgcg gaatagcacc acctccccgc tatctatcct
    13381 atttacgttt cttccccgaa gcggcttccg cggcacggat acgttgcgca cgcactttcg
    13441 cccgcttgag agccgacagc ttgccgcgtg tgcggtcccg ctcggccttc aatcgttcca
    13501 attgagcccg aaggtgcacg cgttcctctt tgtaaatact ggcacattca gcaggagaca
    13561 ggttggtcca gtctcccgtt ccacgtgccc gcttgatccg cgcttccaag gattcacgca
    13621 gcagcctctc gcgcatcgac atcaacgact gcaaggcttc ctgccgccgc tggacttctt
    13681 cctcttcggc cataatgccg cgaatgtcgg ttcctaacat tggctgatcc tcctcgctgg
    13741 ggatatcaat aagaacggct cattctaccc gatttgtatg cctttgttct acctaggaga
    13801 atggatcata acgtattgat cttgcagaca agtatccctc aacatgagaa aaagcgacgt
    13861 tgcagtgccg aaaagggatg cagtatgatc ggcgccatgc cacacatcgg agccttggtt
    13921 cgcgcatggc gagtgtctca aaaatgttcg gagcaggcct ttgcccagcg agctggaatc
    13981 accccgaatc tcttagaatc attggagtct gaacagctcg atcccactgc gtcaacgctg
    14041 gaagccttcg ccaaagctct gcaaattccc ctttcctggc tgttcattca cccgacggaa
    14101 ttcgacctcc tctgcaaaga cgccgaggag gaaccggctc cgctctccac gctaaccggg
    14161 gcagaccccg tgctggacag gttgctcctg gcagccgggc atgatcgaac gctgttcgtg
    14221 ctgctcaccg cattgatgca gagcggcgac ccgaaacttc tccgtgccgc cgaagtcagc
    14281 ctgcggagcc tcgtgaaaca atccaaacag gccaccgtcc catggcaatc ccggccgccg
    14341 ggacattttg agccacccag cgactagctt ccgtcctcct cattcacgcg acgtcacacg
    14401 cccctagggg tggcgtcagc attgcctgca cctcctgcgg cttgtgcggc tcgcactaag
    14461 aagaggccga ccaatgccag ggcgagaagc cccggccagc cgcccaacgg cggatcggcg
    14521 aacaactgaa acggatctcc tgccaacgac ggccacacag agctcaacgc aatgggaact
    14581 gccagcagga taccaaccat cgcttccccg gtgacgagcc cggcagcaca taggagccct
    14641 tgatcgtcag tcggagtctc ggaagccggt ctgccccttc gcactacctc ggccagcaac
    14701 ccgccgaaca tgatcgccgc cgacaatttg agaggcaggt acatacccag cgcgacagcc
    14761 aacaccggga aacgaagctg agcctgacgt tgggcctgcc gctgatccaa ggcgatgatc
    14821 accacgccga gcgccatccc gatgccgacc aaatgccagg gcaatgaccc gccgaacact
    14881 cccttggcaa gattggccat caaagtcgcc tgcggggcac tcaggggatg gggatgcgcg
    14941 gcggtcgcca ctccgattcc atatttcgcc tgtagcaagg acagcaccgg tacgatcacc
    15001 accgcaccgg tcgccactcc aatgacctgc atcacctgtt gcttccacgg cgtcgcacca
    15061 accaggtgtc cggtttttag atcctgcaga ttgtcgccac ccatggctgc ggcgcaacac
    15121 acgaccgccc ccaccagcaa ggcggccgcc ggaccggccg gatgttcacg ccccaggatg
    15181 gccaatagaa gcagcgccgc gagcatgatc gtggcgatcg tgacacccga caccggattg
    15241 ctcgaactgc cgaccaggcc tgccatatac ccggccacgg cagaaaagag aaacgccgtc
    15301 accaccatca gcacggttaa tcccgcgcca gcgaataggc tgtgatccag caggtggcgg
    15361 tacaggaata ccatagggat cagcgaagcc accgtcagac cgatgagcca gaccacgccc
    15421 gcatcccgct cggtgcggag tggcgccgac gcaccacgat cgtcgcgacg agcagcatag
    15481 agcgaaagca actgccggag actgtgcagg atgggccctc gaacttgaat cagcgtccag
    15541 actccgccga cgagcatggc gccgatccct atgtagcgaa tctgcccgct ccacacgacc
    15601 ttcgccgccg ccacaccggt cacatcagcc ggcgtcccca caagggcttg gtacaagggc
    15661 aggagcacca gccagccgat tgctccgccg agaaaaacca ccagcgccgt attgagcccg
    15721 atgatgtacc ccactgcgac cagcgccggc gagaggttga ggcctccgta gaacgtcata
    15781 cgacccagct caagcgcacc ctccaacgat tcactccaaa gttttaagcc gccctcggca
    15841 aacttcaacc cgccccccaa ggctgacgcg ctcaacaaca ggcgaacccg gcgaccggca
    15901 ttgccgcctg atgtggcgcc gatcttcaat acctccgccg tggcaactcc ctcagggaaa
    15961 cgcagccgcg cgtgaatgat caaggcacgg cgtaagggaa tcgtgaacaa gactcccagt
    16021 actccgccga ccaacgacac cgtaaaggtt tgccaatagt cgaagctcgt ccaatagccg
    16081 atcaggacca ggccagggat agtaaagatc acacccgccg ccaatgcctc acccgaagag
    16141 gcggcggttt gcacgatatt gttttctaga atgttggact ggcgaaacag gcgcagcacc
    16201 gccatcgaga cgacagcggc tgggattgac gcggaaacgg tcatccccgc gaagaggccc
    16261 aggtaggcat tggccccagc caagacggca gccagtagaa ccgagagcag gacggctttg
    16321 ggagtgattt ccggcagcga cacggaggcg ggcacaagcg gcgcatcgac aggctctgac
    16381 agccgttcag gctcgtgcat catcgcctca gaagacctgc acgatgaaac atttcagata
    16441 ccgtgtctcc ggcatcccgg ccagcaccgg atggtccggc ccctgccccc gctgttccag
    16501 cagacggatc tggcgattgg catcccgcgc cgccgactgg agcatcatcc agaaatcgtc
    16561 atcactcacc ggttgcgaac aggaacaact caccaggacg ccccccgact gaagaaggcg
    16621 caagcctcgc agattgacgt ctttataccc ggccaacgca tgaggcaggg ctttcttgct
    16681 ccgggcaaag gcaggtggat cgagaatgac cagatcatac cgttgctgat tgcgttccag
    16741 cactcgcaat tcatcgaagg catcggcctg ccgataggtg cagatggacg cgacatgatt
    16801 ctgcgccgca tgcgcttggg ctaacgccac cgctgccgca ctcacatcca ggccttcgac
    16861 cgattgcgca ccggccaagg ccgcctgcac tccaaaggcg ccggtatgac agaaggcctc
    16921 caacacgcgt tttcctttgg cataggccgc tgctgccagg cggttctccc gctgatcgca
    16981 gaaccagccg gttttttgcc cctccgcaat gtcgactgtg aactgagcct tgccttcgtg
    17041 gatgggcacc gtcgtcggtc cctcgccacg caggaatcct ttcgacaacg gcagcccttc
    17101 gagcgtccgg cttttcgcat cattgcgcaa atagacgctc ttcactcccg cttcttgcac
    17161 caggaggtca cccaacaggt cctttcgcag atccatgcca taccccaggg cctgcatcac
    17221 cgcgacatcg gcaaaccggt ccaccaccag gcccggcaac aggtcactct cgccatggac
    17281 aagacgacac gcgttcgcgt gaggggtgac agtacgccga agcgccgcag cagcacgaag
    17341 tcgcgcggca aaaaacgcct catcgatcgg ttcttcgtga aaggtcagaa gccggatgcg
    17401 gatttttgag tgggggttga acaacccgcg tccgaaaaac cgtttctgat gggtgtacac
    17461 atcgaccaaa tcccccgcgg ccggcgtgcc cagcacctcg gccacattgt tgtcgaagac
    17521 ccagagatga ccgtagtaac gaggcccggc acgctcggcg gtcaggcgga ctcttgcggt
    17581 ggaactggtc atagtctgtg tcatggtggt gatgtatcca gggtagaggt gttcaatggc
    17641 atgttggcca tgcggtgcaa atggctcacg agggtgcctg aaatcattcc ctgaagcaac
    17701 cgtgcgtcac gattgttcgc gcgcttgacc ttcccaggca cctatggttt gctgcgagaa
    17761 tagatccctc gcctcgccac ctggtaacgg agcgtccatc cttatgacga cacacacgat
    17821 cggcacggca gttttagatc ggcttcatcg cctcggcgtc cgccatatgt tcggcattcc
    17881 cggcgactat gtgctgagcc tctataaatt gatggagtcc tcgccgattc aacatgtggc
    17941 caccacgcga gaagattgcg ccggctttgc tgccgacgcc tatgcgcgga tcaacggcat
    18001 cggggccctc tgcgtgacct attgtgtcgg aggactcaac acggtcaatg cgattgcctg
    18061 cgcctatgcg gaacgatcac ctgtcgtgtt gctgaccgga tcaccaggac tctcggagcg
    18121 cgcgcgcaat ccctatctcc accatatggt gcgggagttc tcgacccaac gtgaggtgtt
    18181 tgaaaaaatg acggtggcgg cggtcagcct ggacgatccg ctgacggcgg agcgcgaaat
    18241 ggatcgcgcg tttgccgcct tgctgcgcta tcgacgtccg atctatctcg agatcccgcg
    18301 cgacatggtc catgtgccgc tggcgtacac gtctcataaa acgagcatct tagacgaacc
    18361 aacagacaag gccgccctaa gtgaagcggt gaccgaggtg cgcgtgatgt tggagtcggc
    18421 caaacgtccg gtgatcttag ccggcgctga agtgggccgc tttggactcc acgacgagct
    18481 gacgaaactc gtcgaacggt tgaacgtgcc gatcgcctcc accctcctcg gaaagtccat
    18541 catccgcgaa gaccatccgc tctatgtcgg cgtctatagt ggcttggtcg cccgtgacga
    18601 ggtgaaggaa tttgtgaacc aaacagattg cctgctcatc ctcggttcga tcctgtcaga
    18661 tgttgaggat ctcgacgccc gcagcgccct gttttccgat ggccatacca tccacgccac
    18721 cgcggatcgc gttgccatca aacatcatcg gtacgattcc atccgcttcg aggatttcgt
    18781 caaagcactt gccaatgccc cgctgccctc ctttcccaaa ccacagctgc ccgctccccg
    18841 tgctccggaa gaacctatgc ctccggcaga cgcctcggtc agtctgcacg gcgtattccg
    18901 ccatctcgat acggtgctgc aggagaaaac actggtcatc gccgatgtcg gcgaatcgct
    18961 ctttgcctcc gtcgatctgc acgtccaccg gcgctttgag tttctctcgc cggcctacta
    19021 tacgtcgatg ggcttcgccg tcccggcggc cgtcggggcc tcgtttgccg atccgtcctt
    19081 gcgccctatc gttctggtgg gcgacggggc gtttcagatg accggttcgg aactctccac
    19141 cgccgtccga taccggcaag cgcctgtcgt gatcgtcctg aataatcacg gctattccac
    19201 cgaacgcgag attctggaag gaccgtttaa cgatattcac gaatggcgat acgaacggat
    19261 ctgcgacctc attggagggg ggcagggctc gcgcgtcgcc actcatggtg agttcgtcga
    19321 tacgctggcg aaggcactgg ccgatcccag ccagccctat gtcatcaacg tcttactcga
    19381 tccctccgac cgttccccgg ccatgatgcg tctcgccaga cggctggcca agcgtctctc
    19441 caccgaccgt ccgtaggggg attctcccgt cgaaagccac cccgcccctg cgtgaggaac
    19501 cgcaccgttt tccatctgcc tccaagcctg tttcaccctg atacaatgcg gggatcgccc
    19561 gcctccccca ttgacccctc atggacaaat gtggtctcct aggagcggac ttgtcgaatt
    19621 gtcatttgtt ggggaattcg ccactatgtc tcctaagatg ccggacggaa agacgatgcg
    19681 cgacggccaa cctttttctg cacgactcaa caatgtcgtg accaccgaac gggagttcga
    19741 cgagttggtc acgaagtcgc tgccggaatt gttggaccgt gccaccggtc agaccaagcg
    19801 cttcctgcgt gaaacgaatc aatggggcga cgatatcacc catgagaaat tggcgcttcg
    19861 ctggggttac gaattagtgg agcgttttgt ggtcttcggc cgcaccgaag ttccctgcag
    19921 gccgtttttt ctgttggata gcctgatcgc gaaatcgttc agccagccag atcccctgtg
    19981 ctaccacaag gagttgctga cgccggtggg gcggtttctc gatgggttgg cttcccgcgc
    20041 cgtcgtgagc cgcgatgcgc tgatcgcgct gttttatcac ttctacgggt tcagccaggc
    20101 ccatgtggtg aagctgctgg gtttcggcca ggcggaaagc cagcgcgtct ataagaactt
    20161 cgagcggtgg cgccaatcgg gctggcagcg cacgatgaac gaaacgggat tgagtggccc
    20221 ggaaatcgaa attttggagc aagaattgcg cacgagaccg gaccacctca ataacgaagt
    20281 ggatcggctg atgcccgtct tgcaatcgca ttatcgaaag agcgaaccgg aacattatcc
    20341 ctgcttgtcc gaacagaagt gggggcaact ttttcacgat gactacggcc acgactaccg
    20401 cgtctggcac ctggcgtttt gccgcgattg ctttctggaa gtcgcagatc gccggcaagc
    20461 cgacgtcagc agcggggtga agccgcaagt caatctccat atacatccgc tgaaaaaggg
    20521 aggcgtcctg gctctctttg gaggcgatcg gggaggacgg caccatggaa acaccagagc
    20581 tcaacgacta tcgcgcacat ctgcttgacg cgctggatga ccagccgcgc cgcgcggcgg
    20641 aagaacgccc tgggtttctc caggtcctgg tcaatcacga gcgcatcggc acattgaatt
    20701 gctcacagac cgatccatcc ttcgcctata cagcccgcga acgcagcatt caacacctgg
    20761 aactccgaag cgagtcgggc gtgctgatcg gagggtgcgt ggcacccgaa gccggccaga
    20821 aaagcgtgcg gatcccggtc ggcaaaggca gcctcacgct ccatgttcaa aaccatttcg
    20881 acggcggttc gctcaacgtg cagtacgagg tcgcgccggg ttggtggtca catctggcca
    20941 cagccattgg cttgcccgcc ggcgccgccg tagcctcgac gcaaggcagc cccgtgtgga
    21001 gcatgaccac cgccttcgcg caggtcgtcc tggccgtggg cgtggtcgct ctgttggctg
    21061 aacggattcc ggcctgggtg ggctcagaag atcgtgccca tcagttggaa gaagaattag
    21121 ctgcgcgaca atcgacgcag gatcagatcg atcacgtcca ccagcagctc gcccagctgg
    21181 tcgagtcaca agcggcagcg gtggccgtgt cgcgcgccga gcaggatcgc attgcgcaat
    21241 taaataccct catcgacacc gtcgcccata cgcagcagcg ccttagcacc caagtcgtcg
    21301 ccgttcagga agacctcaag accgtgaaaa cggatgttgc gcaggaagtg caaaccggca
    21361 tgcgcgtggc cgtgactgcg gtcgaagccg accgcgagct cgtgcgacag gatcttcaag
    21421 cggtgaagtc ggtcaatgaa acgctggtca aacaggtcgc gttgctggag agccgcaatc
    21481 gggagttgca tgcccgtctg gccttgacct cattggaagt cgccaaagcc aatgcggcat
    21541 ccaagcccgt cgtcgtcgcg aaaaacgagc cgccgaagga gacccctgcg ccgaccgcag
    21601 tcgcggtgtc ggacaacctt cgcgacgcag atcgccaggc cttcatgttc tgggtggctt
    21661 ttcaggacgg caccaccgaa aaaagcattg aagatctggt ccaggaactt aacgggatca
    21721 agaaagggcc gatgaagtcg gggtggtatt ccgtcgaagt gaatctgccc aatcccgagc
    21781 ctccggatcg ctttctggag tctgtgaaac gagccaagat cgtgaagtcg gtcgtgacga
    21841 gcaaggctat gcccccggcg cagtaacggc gatctcctct cagcacattc acccgctcgg
    21901 cacggttggt gtttgcctcc agtgatggaa tcattcccta ccgcaccggg cataaacgaa
    21961 tacagccacc ctctccccgc cccgcacagg agggaccatg tcggattttc atcagaacgg
    22021 tctcgtgacc gtgttgcacc gcctcggcgg ctccaacctt gagcaattgg aaaaggagtt
    22081 ggagcggcac gcggcatcga acccgatcgc cctcgtcctc ccctcccttt attccgaact
    22141 ggaaaaccct gcgctgaaac atatcgtcca ggtgttgaaa gacatccggt acgtcaatga
    22201 aatcgtcatc tcgctggatc gcgcatcggc actggaattc cggctggcca aacagttttt
    22261 ttccgtgctg ccgcaacgag tgcgaatcgt ctggaacgac ggagccggta tccaggacat
    22321 cttgaaattg ctggcccaag ccgaactcga caccgggttg cagggaaaag gccggggatg
    22381 ctggatggcc ttcgggtatg tcctggcccg gggggaaagc aatgtgatcg cgctgcacga
    22441 ctgcgacatt ctcagctaca accgcgaata cctggccaga ctctgctacc cgatcgtcaa
    22501 caccaatctc ggctacgaat tctgcaaagg ttactacagc cgggtgacca atcgccttca
    22561 cggccgcgtg acacgactgt atttcacgcc gctgattcga agcctccagc aagtggccgg
    22621 ggctcaccct ctgctcacct tcctcgacag tttccgttac ccgctggcgg gggaatttgc
    22681 catggtgcgc gacttggcct ggatcaaccg tgtgcccggc gattgggggc tggaagtcgg
    22741 cgtgctgtct gaaatctatc gcaattgcgc gctcaggcgg atttgccagg cggatatcgc
    22801 cgacgcctac gaacacaaac accagggact gtccgcggat aatgccgaac agggcttgca
    22861 gaaaatgtgc gtggatatca ccaagtcgct gttccggaat ctcgccagcg agggtgtggt
    22921 gttgtcggag ggcgtgctca agaccttacg cgccacgtat cttcagtcgg cccaggaagc
    22981 catcacgcga taccaaaacg acgcggccat caatagcttg cagttcgacc gccacgctga
    23041 gcggatggcc gtagaggtct ttctgaaagg catgaagatc gcgaccgaag catttctgga
    23101 agacccgctc ggcgtgccca tgatttccaa ctggagccgc gtcaccggag ccatcccgga
    23161 tgtctttgaa cgtctcatcg gcgccgtcga gcgagaccac gactgggatc ctgtcggaga
    23221 ccgagtgtaa gagagatgtc ctcctgcgtg gaggcgattc accacctgac gttccttccc
    23281 cgacacacga aactcttctg ccgtcgcatc aggccatttc tcttacctgt gcctcgcatg
    23341 gattgccgcg acgtgggccc gctataccgg aatgatctct tccgttcgct tggctcctaa
    23401 taccaataac accagcgaac gatccgtgag caggtattgg gttcccatct ggaaggtttg
    23461 gttctgaggg cgatccggca gattggtatc aagcagcagt gtccattctg acgcaccgac
    23521 acaagggggc aaggtaaact ggaccggctc atggtggcca ttgatcagga ccagtaccgt
    23581 gacatccttc cccggttgtc gacgtccggt tgtcggcgcc cggccgtcca agagcattcc
    23641 aaaacagtgg atcgtcgtat cggtccagtg atgctcttcc atcggcccgc cgttggcatt
    23701 gatccaggtc acatctcgaa cgccggaatc ctcggccagc tgtccggtca aaaagagatt
    23761 gcggcgtaaa atcggataat gatgacggaa cgcaatcaat tctttgacga attgaaagag
    23821 cgatgatcgt gtttcgagca acgaccaatc gacccaacta atctcgttgt cctgacagta
    23881 ggcattgtta ttaccccgtt gggtgcggcc aaattcatcg cccgccaaca ccatgggcgt
    23941 cccttgagaa atcaataacg tcgccatgag atttcgcatt tgccggtcac gaagcgcgtt
    24001 gattgaagca tcttccgtcg gtccctcggc gccacaattc catgagcgat tgttagagga
    24061 cccgtcctgg ttatcctccc cgttcgcctc gttatgcttc tcgttgaagg ccaccacatc
    24121 atgtagcgta aagccgtcgt gcgccgtcac aaagttgacg cacgaccagg gccgccggcc
    24181 ttgatgattg aagacggagg cggatgcgca gagtcgatcc gcaagattgg tggcagcgag
    24241 ttcgccgcgc cagaaatcgc gcacatcatc ccgaaattta tcgttccact cagcccaccc
    24301 cggaggaaat ccccccactt gatatccccc aggcccgcag tcccagggct cggcaatcag
    24361 cttcacggta gccagcaagg ggtcctgact acagactttc aagaagccgc ttcgattatc
    24421 aaatccattc ggctcgcgcg caagaatggt cccgagatca aaacggaatc catccacgtg
    24481 catttgttgg acggcatagc gcagactatc ggtgactaat tgaatgacgc ggggatgact
    24541 cagattcaaa gtgttccccg tccctgtatc gttaatgtaa tagcgtggtt gctcaggctg
    24601 gaggcggtaa taacttgagt tgtcgatccc tttaaaggat agcgtgggac cgagctcatt
    24661 gccttcggcg gtgtgattgt agacgacgtc cagaatcacc tcgaaaccgg catcatgcag
    24721 ccgcgcaatc atctctttgc attcgcgaac actttccggt cgattcgcgg cataccgcgg
    24781 atccggcgcg aaaaatccaa tactgttata cccccaataa ttggtcagcc ccttctcaag
    24841 gagatgactg tcattgacaa acgtatggat cggcagaagc tctatagatg tgacccccag
    24901 cgactgcaga tagtccagaa cctccttcgt cgcgagcccc tcgtaggtac cttggagagt
    24961 ggacggcacg gccggatgaa gtttcgtgaa cccacgcaca tgcatttcgt acacaacggt
    25021 gtcatcccac ggaatcgtac gccgatctcg aggtcctgac caatcgaagt tttgatcgac
    25081 tacggtgcac ttcggcataa atggcgcact gtcacgttca tcaaatgttc gatcatcgcc
    25141 ggactccatt tgatagccaa acagagccgg attccactgc agttcgccca tgtgggcata
    25201 ggcgtaggga tccagcagca gcttgttcgg attaaagcga tgaccggcgt cagggtcata
    25261 gggaccgtgg acacgatagc cataaggcgt cccagggtgg atcccggcca ggtatccatg
    25321 ccagatctga tcggtgtatt caggcaacga cacgcggtcg atttcttttt ctcccgttgg
    25381 gtcgaagagg cacaactcaa ctttcgtcgc gtgggcggaa aacagactga aattggtccc
    25441 ctgtccgtcc catgtggcac cccgaggcgt ggggcgccct tcgagcactt ctgtcataat
    25501 gatgaactcc tacatcctgc taaccgaacc attcaggggc cgtgctccaa aagcaacgag
    25561 attcctctgc cctccgtcga ccggcgctga ttactcacag tccggatgta tccacgcgca
    25621 ggcagcctgc ttcgcctaac ttctcaacgc tcctcgtcga ccgctcaagg caatgacgag
    25681 cacgatgagg aggagaagcc ccagtccgcc tgaaggatag tacccccacc cactgctata
    25741 cggccatgtc ggcaggacac ccacaagcaa cagaatgaga ataaccagga cgatgttagg
    25801 catggaggac tcctttcagt gagatgtctc acaaggtact gggaaactag gtagggagag
    25861 actaggaaaa tcccctggaa ggaggtcaat gaacccaaga accgggagtc tacagccgtg
    25921 tctcctgtga tcggatcaac gggcccgtat gttcggatag ccccttcatc gcagattgaa
    25981 tggcgccgta cagttgatca ccggcggctt ccttcgggag caggactgac gcccccgcgg
    26041 ttcgcatggc ctcctgattg tctactgaag cattcaccga cataccaatg acgatggtgt
    26101 ccggaagggc ggttcgtatc tgcgcagtgg cttcgatccc gttcatcagc ggcatattga
    26161 tatcgatcac caccaccgac ggcttcaatc tccgcgctaa ctccagcgct tcctgcccat
    26221 tggcagcctc agcaatcacc tcaatatccg gacaggcatc cagcatcaga cagagcccct
    26281 gccgcaccat ggcatgatca tccgccacca ggacccgata gggaacggac ttgcaatgac
    26341 cggtgctggg tgcgcgcgcg acatgcgcgg acggtgcaat gtaggacaat gagatcccag
    26401 tcgcctgctc cgaaagctgc gtgatcggca acaccagcgt ggctttggtt ccttcgccga
    26461 gttttgaacg cagctccaag cggccgccca atgcttccat acgttggcga atactgaata
    26521 ggccaaactt ggacgacatc gcggcgagtt tttgtgacga caacgtgatg ggatcaaacc
    26581 ccaccccccg atcctccacg acgatgctga gcactccatc ttcactggct gccgcgatcc
    26641 acgctttctt ggacccggcg tgtttcactg cgttgagaag taactctcga accgattgaa
    26701 aaacgagcac ggcctgattc tcgggcaatt tcaattcttc cggcgtgtcg atacggacgc
    26761 tgacctccaa gtgataccgg cgcatctgtt cgcccagcca gcggagggcg acaaagagcc
    26821 cgaactcatg aagcacgggc ggactgagtt ccgccacgag attgcgcgta taggtcaggg
    26881 attcgtcgac gacgtcttca gcctgtttga tcatttggat ggaatgagac aaagggccga
    26941 ctttggcctg gcctaatctc agccgaacca gcaccagcat ttgcgccaaa tgatcatgaa
    27001 gctcggcggc cagccgtgtg cgctcccgct gttcggccaa attgagctcc gtggccaacg
    27061 cccggagctg ttgttgcgaa gccaccagtt catacgtgcg ctcggagacc ttgcgttcaa
    27121 gctcctccgc gaattgctcc aagcggaact gcgaagcctt ccgttgcgtg atatcgttga
    27181 agaggaccgc gaccttttgc tcggacggca ggccgatgcg ccaggcatat atctcgtaat
    27241 ggcgatccag cgccttcgcc tccttctcga accgcaatgg ctcaccagtc gtggcgatgt
    27301 tcccgtaaat ccggaaccaa tgctcttcat gttgtggcac gatatcccgc atgtggcggc
    27361 ccaccacatc gcggagccct gtttgcacgg caaacgccgg gttgacgagc aggaagcggt
    27421 agtcgacagg ctcctgctgc tcgtcaaaca gcacttctat cgtgcagaag ccctgatcga
    27481 tggattcgaa cagcgtccga tattgctgtt cggattcccg cagttgctgt tgcgacagtg
    27541 tctcttgctc caaaagatca cgcacctgat attgacgccg ccgcgatcgc aatccgactt
    27601 cgatggagcg catcaacgtc gcctcgccaa tgggccgttc gagcaacgta atgctgccgg
    27661 ctgcctcggc gaccagatcg aggagacgtg ccaagcggga ttctccgcct cgcgtcaaca
    27721 caatcatcgg caattcgctc cacggcggct gcgccttgag atggtggagc aactgggaaa
    27781 tatgaggcag ctcgagggat tcttccgtca cgagcaggac ggcgccaccc acagccagct
    27841 cacgacaggt gtccagagcc gttgcgcaga cgatcgcatc cacaccttgc gagatgagca
    27901 cccttgccat ggccggagcg tcctgaccga tgggggcaag gatgatgatg cgctcctcaa
    27961 gcctagttcg caccatcagg gcgctccatg acctgttcga gggcgccgta gaaggaaggg
    28021 gtccccgtca ggacaccttg aaattgcttc aggggtgggc cgatacgcaa cccgccgtct
    28081 gacaccaatt gatattctct gatggttttt tcatgccccc cgcttcgctt cttgatcacc
    28141 gacaacgcct gccgaactgc gccggctgcc tcaaaaaatc gcacgctcac gaccgtgtca
    28201 gcgagatagc tcagatttaa cggagcctcg gcctgcccga tgagcccgta ttgggccaaa
    28261 ataaggatgg tgacgacgcc tttctggttg agataggcgg acatttcgtg gagctggttg
    28321 acgagatact tttcaccagg catcgcattc aaatatccat tcagactgtc gatgatcagg
    28381 agggtacagc caccctccac agcctcgcgg atgcgcatgc cgaattcgcc gggagaaagt
    28441 tcggcgggat ccacctgctg cacatcgatg agacccgtgt tgatatgccg ctcgacatct
    28501 aacccgacag cagtcgcgcg cgccagcaca atgcctcgaa tttcatcgaa tgcaaacagg
    28561 acgctcgatt catttcgttg cgccatttgt gctgcatact gcatggccag cgtggacttt
    28621 cccgttccgg ccggacccat gatgagcgtg gtggttccac gatcgagccc gcccccgaga
    28681 agctcgtcaa actcctggtt gccgctcgag accgcctggg gcgaaaaatc agcccgatga
    28741 tcggaggcaa gaatccgggg gaacactcgc aggcccccct tctcaatact gtaatcgtgg
    28801 tagccttcgc ggaatttcat tccacgcatc ttgaggacac gcaaccttcg ccgggaccgg
    28861 ccgtaatcgg gcgacaactg ctccatttcg atcacgccat gggtgaggct gaggacatgg
    28921 ggatctccac cgatccggct attgtccatc ctgtcatcca gcagcagtgc agtgcagttg
    28981 tatttcgcca gttcttgttt gaggttgatc agctgccgcc gataccgtaa cgcggtttca
    29041 gccatcaagc gaaactccga cagcgagtcg aataccacgc gcgcaggttg aatacgccgg
    29101 atttccgact gcagcatttc gctgactttg gtgagctcga cttccgaagg gtgaaacacc
    29161 gtcgtccgtg cctcaggccg tacaagcgaa tcgatggccg agagttcgaa gagcgaaatg
    29221 gagtccagcg accagccatg cgaacgggca accgtgacca gctcttcttt cgtctcggag
    29281 agggtgatat agagggtttt ttcccccaag cgcacgcctt ccagaagaaa ctggagcgca
    29341 agcgtggtct tgcccgagcc cgggtctccc tgcactaaat acatgcagtt ggccggtagt
    29401 ccgtcgccaa ggatttcgtt cagtccgtct atgcccgttt cacagcgact atcggcctgc
    29461 ctggttggat cagtcatggt ccagcctcaa aagagtgagt gatcaatcac ggccatatga
    29521 aaattcttcc gaaaatttat ccgcagtata gcgtggagaa aaaccgagcg gactagaaca
    29581 atccctagtg cccccctcga taagagatag tcgtcgaacc cgaaagccta gggtgttagt
    29641 ggctgaaggg gtcttatgct ggaaaatcga tcgggcatga cgcaccgcgc ggaggatgca
    29701 cacatgctcg agatacggag gacaggatca tagaaagaaa actcaactag cttccaggca
    29761 attaaaagaa cggggattaa ccgcgaagaa tgttttcgag gcagacggtc ctaccagggg
    29821 tctgaattgc ggtttccatg gtggaagcta tacttcctgt aacacttgcg aggaggatgt
    29881 gatgcccaat tggctcgctt atgccggagc aattcttacc ggtctgatac caccgttgat
    29941 catgtatttg atcggctcgc aaaagaaggt tttaggctat gcccttaccc aaaacgaacc
    30001 gatgatcaac ctccgacatc cagaccttga cgggcgactc tcaatgtcac tcgatggcaa
    30061 gaatctgaac ggatgtcgga tcatggggct ggtcattcaa aacctgggga tgaaggatat
    30121 tgaagagcaa tcggttcacc tcacatttcc cgccggagtc accgtcctgc atgcgcactg
    30181 ttcgtcgtct gctttggcga tcgggcctaa aatcgaggcc cgccaggctg attcgcatga
    30241 ggtccgcatt cccctcatga acccagggga caaactgatg gttcgtctct tgatgacggg
    30301 aaatgaagcc ggtcgtattc tggtgacggc caagggacca agcctcaaat tcaagccatt
    30361 cgatcccacg acattcattt cgccagcggt gaatcgtggg cttctagtca tgagcggggc
    30421 catctttgct tggattgtct gggcctgtgc gggaaccgat cgattcatgg ccaggccatt
    30481 ttgggataaa acgattacgc ttgcgctcat tggcttgttc ttggccattc cctcagcgat
    30541 cttggcttac caagggacga ttcgcctgtt aaagcgggcg ctgcaatgga gggaaggtaa
    30601 cagggcgagc tcgcttcaac cccacccaga gctctgatcg taagtcagcg gcgcttcatt
    30661 ttgcgaagcc gcacgaaaaa aaacgacagt gattaaacga cagtgattaa tggatatcgg
    30721 ccgcaaacca tgtcttgtca agaagctggg ggaaggggct tctcgacgtc tgagaaccta
    30781 ccctttcgcc ttctgcgcga ataccgtcgc atcacaggcg atgtactta
//
LOCUS       DAOQDS010000050        28600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_567_length_28600_cov_14.330426, whole genome shotgun sequence.
ACCESSION   DAOQDS010000050 DAOQDS010000000
VERSION     DAOQDS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 28600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28600
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_567_length_28600_cov_14.330426"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            571..1704
                     /locus_tag="PK589_12960"
     CDS             571..1704
                     /locus_tag="PK589_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNJ20563.1"
                     /translation="MGLTKRKDSYYVAFSVIVSDDGKTFKLAPGVPGARLKRWKVGCL
                     NKTIAKEMEATIRINLLQGKILTEESRPVLFSEWAEQYLDLEEVRRLRSFSTRAIHVS
                     HLLKFFRDKPLSAFTCEDVRKYRSWRAGQGVSVQTINHDHMTLTRMLNLAMSDQFNLI
                     SRNVSASVPKPDPKNERDRIVTPEEWAAIRAHAAPHLARFLTVAYDLGPRRGELLKLE
                     WSDVDMKRREFTLRETKNGEARTVPMTPEVYEAFRQCLKERRLDTSRVFLYKGRPIQR
                     RLTTAFKAACRRAGIVYGRKNGGITAHDFRHSAATSLRRAGVDTMTAMKIIGHKSEKM
                     HRRYNQISPADLQRAVDKLVIYQADQASGADTVLTHEKLHHEA"
     gene            1734..1808
                     /locus_tag="PK589_12965"
     tRNA            1734..1808
                     /locus_tag="PK589_12965"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1766..1768,aa:Gly,seq:ccc)
     gene            complement(2104..2823)
                     /locus_tag="PK589_12970"
     CDS             complement(2104..2823)
                     /locus_tag="PK589_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ20564.1"
                     /translation="MSRKEEEEEEERATTVVAIVSNNYLLRLGLQRIVEQEKWLRLIG
                     QTANVTTFDDVIARERPQIMIIDTEIESDVPGLIQKIKATAPETKTILLTGMDQAECT
                     RQAFACGVDGLVPKIQPSPVLIATIDYLARAAAAPVTFTGSNTAPLNKPHRPTIHTAI
                     ATNPAQSKWPDGLTEREQEVVRLVSEGLSNKDIANRLCISSITVRHHLTSIFDKLGVS
                     NRQKLLLRAHQNGLTRPPALA"
     gene            3899..5632
                     /locus_tag="PK589_12975"
     CDS             3899..5632
                     /locus_tag="PK589_12975"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNJ20565.1"
                     /translation="MKAVIMDGAPWAGTMLAFPVGNELLIDHLLSAVKRLGIREVAVV
                     SCDQDSYVRDLVCRAGHSYGLTVLWRTEQVYRGTAGSLQAVEDFLDTPTFLGIHANVY
                     LRDVDLAAAFKAHDEHRADITLVTQRMAGTDDDLESVEVDPAGLVSRVNILHWSRNRR
                     SQLTPCGLYVFSREVLSQIPRDEYFDVNEQLVPLMRNRGGVIRAHQIEGGVHTIVGPD
                     DFLWLNREILHKDIQGSGHGNGRLGGVEDIVIGENSEISPHSFLLGPLVIGPNCVVED
                     YVHLIGPAVIGPTSYLEKGSLVRESVLRSGTRVQGNARVEYSVIAGDETVPEGMRLRS
                     AFWSTAKPALYEPHGVPAQVSSNRMLQRSRYSQRPARRMQGGTGQRSIYGMVKSVVDV
                     TGAAVGLLFAGPLMLLIACAVKLDSPGPVFFSQKRCGKNGKEFWMHKFRTMVPDAEQR
                     QKDLKAQNSVDGPMFKLQEDPRITRMGKLLRKTSLDELPQLLNVLRGEMSLVGPRPLA
                     YDEMKFCPTWRDARLSVAPGLTGLWQVKARNRNRFSEWIRYDLEYVRTHSLSLDLYIL
                     IRTARVLLKGV"
     gene            5632..7866
                     /locus_tag="PK589_12980"
     CDS             5632..7866
                     /locus_tag="PK589_12980"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNJ20566.1"
                     /translation="MNVPYTTGLPDVDSTQTVGRSSFGVREHLITIFRQKRKLLTILG
                     MATLVAGPGSFLMTNIYSAEVRLLIQNARTPFSMSTPLTGQVFTPSDITQKDDVATEV
                     QIFTSPILLDKLVDYFGNERVLAAMRGRWDWVGEIPKTVFKQLSGLPPVAKLLTTIGY
                     VPKPEDLHYQAVQKIRAHLNVEGVRQTHVFVASLDSPDPEFSADALNALVGIYMDHQL
                     SVRKGKGAREFFDEQTKQMRGELQQAELRLQAFKDKWNIVSIEDQKRHLLQQVTHTEA
                     ALRESQVQVAETEVRISKLKERLAGQQEAIPLTNVSERNPMLDQLKNRLMQMELEYSQ
                     YVPDSPAAAELVREIAAVRARLQAESSKVTGAATSGLNQNYQELKRTLVMEEGRRESL
                     RPRLSELTKQFKSYRDSLNTLDQREMELQGLMREVKVKQEAFDIYLKKEEESRINEVL
                     DRKGISNVNAIEHATAPQKPVQPRKFLNLVIGLLIGLVGGFGSAYVSEYMRRTFVTRE
                     EAEDNLGRPVLAALPLVRPDTSDAGSFDLELRQAAQHVIRSYHERGLRTLLVTSALRG
                     EGRSHVAGALARVLAEQKFRVLLITFEEAGAEKLNTGDAAAGAKQATDTGRFRQDIPE
                     ATDRIHLHRLLVRHREGTALEFAEHLAEVAKTMRDRFDLIVIDGPALTSFPEMRVAIA
                     GIDGTILVVEAERTVSVAAARTTTAIEAAGGHLLGLVLNKRRYIIPEWIYGRWLAAGG
                     REGV"
     gene            7863..8657
                     /locus_tag="PK589_12985"
     CDS             7863..8657
                     /locus_tag="PK589_12985"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ20567.1"
                     /translation="MSESIAGVMQVDEKIVPAEGFVREDGWYAPYDYWLTLWGLYDLY
                     AYALALLGDVKGKVLLDCGCGPGHTSVMLAKRGAIVTAFDTADGQLDTARRLAQANGV
                     NVTFLCKPFEELDFPDESFDVVFGTFVLHHVDLPKASQQLARVLKPGGKAVFIENSAL
                     NPLLMAARSKVCGRLGVPQYSDDHEHPLTRRDIATLQSAFPGACTIHFPSFLFFRLLD
                     FYVFKKRWSWMTALLRHCDQLAGSIPIVRRYGYLQIVELGKGQGRA"
     gene            8650..9924
                     /locus_tag="PK589_12990"
     CDS             8650..9924
                     /locus_tag="PK589_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNJ20568.1"
                     /translation="MRSKPSKFGRGFCCALLLLSGLFLVATDARSAPATEAVAGCRTE
                     APALKHHRASAVDRVTLTSPSNGVYIGLYSIGTIPEDRLRFTAKSGHRPAIVFTFHDW
                     VSDDDWTASSPHFRTFTDPLESSRLSPLQLAEQVRSEGGVLAVAWAIQCCDWESHLFW
                     YGFRKPTVTVDRLLRGDFDSYITSVARQIKAYRHPIMLTLFSEFNYQGMMGFGKEGRT
                     RLDDVDHVCRLYGDPTWPDGPERIRDAFIYVIDRFRNEDVRNVTWFMYAGSHYMNPQH
                     EDYSPWLHPQYFYPGDDYIDWVGQSAYFIDTRSVPKLRQQELATSFAGALGPGYAAWG
                     AVTDRPLFLPEFGVLGDKTSSRARVIEEVFRDVLPQFPRVRAITLADFKIAEDYYEVP
                     RFGTFEDETEAWKRAVRQNPRYLKQASFKGGE"
     gene            9932..11308
                     /locus_tag="PK589_12995"
     CDS             9932..11308
                     /locus_tag="PK589_12995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNJ20569.1"
                     /translation="MALKRSSQWLCLCAIFAVCFTLRIGVGGVIDATVSVVKRADVYS
                     DIAVNLARGHGFVVVAGGEPIIWRAPLYPAFLAQVYALFGEYSETGVFLAQSALDSFT
                     AVLIFYMGTRLFSETVGLLSAVSFALHPLSAYYSLRFLSEPLFTLAFTALISAWIAAT
                     TTRRLMAYTVVGALIAVAALVKPVALGLWPLLATCAVWQLRDEPVRALSAVTGLTLAC
                     LMVLAPWALRNYRATGEVVAVATGGGYALWLGNQMVSEGKEDWEVDEVTRAHLFERRG
                     TVITGAQPSDHALAGYPSIRSATQPVHISVPEDHAFFIAAWREMSSHPFDTALLTVRK
                     FFRFWFRIFLPDNRWAQSYIVVFQTLFLSVAVLGMLEAKRQGITVFALILPVLFLAAV
                     HAMTFSTIRYSIPTIPVLSILQAAGLRAIVHALNERWGLSLGVSWVRWLKAALVGSAA
                     RSYRGTAL"
     gene            11355..12584
                     /locus_tag="PK589_13000"
     CDS             11355..12584
                     /locus_tag="PK589_13000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ20570.1"
                     /translation="MKPIRVVIIRNCRGITGMTGGETYLLSLLKGFDPQKVRCLLVCI
                     VNPALGETSWLRELKQTGFEHVTIPIGNPFNLSDVYKVSSLIRSYEADVVHALDHRSD
                     LIGILSARLTGRPVLASFLGWVNFPKGSWRATVYPWIDRMILKRLDAVITDSVAIGTE
                     LKMREQDPPVVAIRNGIDTRWFDPTRTLTPFSQESFLKGGHFVFGMVGRIHPVKGHLN
                     FLKAARTLLETHADCRFLIVGTVLPGFEHYRQGLQDFIREQGLEQSVLITHVSLAEIP
                     AVFAGIDVLVAPSFAESFSFTLLEGMAMEKAVVSSEVGGAGEMITHGETGYLIPVDDV
                     SRLTETMRMLRAAPEKVREVGLRARKKIVNDLGIEAMAARTHQVYRSLIEFKGKTSGG
                     AGDQGRLREQLAMISAK"
     gene            12621..13577
                     /locus_tag="PK589_13005"
     CDS             12621..13577
                     /locus_tag="PK589_13005"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ20571.1"
                     /translation="MKILITGSQGFLGKSLAHYAVRNGHHVLGVSRSDADGLIAGMES
                     IRCGLDQAGFVDVLNGFAPDVVVHAAGSASVGYSFQQPREDFLMAVETWASVLDAVRK
                     AEGNPLVIFPSSASVYGNPTRLPVPEGAGLRPISPYGFHKVICEQLAQEYAHCFGLNV
                     VSARLFSTIGPFQQRLLVWELFRQAIDANPYLTIQGTGTETRDFLHIDDICRYFLGIA
                     EREPKGFWAVNVASGESTSVRTMAEMVTRLVGSTQAIVTLNKDLPGDPKQWQADTTLL
                     QELVPYQAPDIANGLEDCVRHWLSEPSLSAQRELRCSVEGHS"
     gene            13704..14990
                     /locus_tag="PK589_13010"
     CDS             13704..14990
                     /locus_tag="PK589_13010"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNJ20572.1"
                     /translation="MKALSQQTAVVFAGKMTGACLGFVMSLLVARWMGPEEFGLFSLF
                     IVILIFGNDVLGDGLSPGVVRFYSMYRRADPSKAAEVLTNALALRMLLGIPVVVVGVA
                     VGSQYAARVFHSQLYVLPVVLGLVGSFGAALWSFNLSVWQAREEFGTYGVMVALINVL
                     RVLSLPLLFLGGYLTLGGIMGSHVLVYFLCALSGTWALRRHLAHVRVNGELLRELFRF
                     SKWPAMASLCFILQVNLGVPVLNYFVNAREAGLYGAGSSLLMGVDFLTVSLLTTLLPK
                     VSQLTGIEQCRSYVQRSFPMYALIAAAILPLLFFARPVVIGLFGSAYEGTIEVFQVLF
                     VGTLGTLVTHPLYLVLYTMNRPHLHTLSGIVGLIVWLLAGFWLIPQFGAVGAAWTTLC
                     SRLVQSLMIVVILWHALGLGGSSGLARSVMPDVRGS"
     gene            14987..16483
                     /locus_tag="PK589_13015"
     CDS             14987..16483
                     /locus_tag="PK589_13015"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNJ20573.1"
                     /translation="MKPSDQTYTIPTLLGATLGASVLALSTKALAIVIACSAFLSMTA
                     SAVFPYYILVATIPVQVDLLGGLTVTKLITPLAIGMVTINALIHRGPWPVLLRWPAGY
                     LAGAFFLASLLSLVLAEGLRGLPGEAAKIPVYGALFFFTLTFNRHPEDMRRLLWVITI
                     TGVGEALITAAQVHYGFVMPGDWRRNVGLPSEGGVDGAFMSISEGKVRAEGTTAHPIF
                     LASFFLMSMPCTAFLLFQEKHPWVKAALGAMLSLMAYAWFYTFARSSVIGFALLILVG
                     LWLQFKFVRKLSLIVLVLVLAGGLSYQVVSEWFQVGVQTVENQNLFGKADLNEGSGSF
                     AFRRESIVGGWNLFLAHPWFGVGFGQAINHYVKYLPAWADHYYHPAVIHNMFLEVASE
                     LGVFSLSAFIGLWVVAFVSLKRGLRIPDVRPYAVLLSSILAGQMIFLMITPMVREIWL
                     TLPMAIAIGHMAKTHTRATFGIPFRQVGARAQLRRIVDRGNGLPAGLR"
     gene            16491..17495
                     /locus_tag="PK589_13020"
     CDS             16491..17495
                     /locus_tag="PK589_13020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ20574.1"
                     /translation="MKIRVSVVIPAYNATKTIGDGLESFARQSFPAAEVEIIIVDDGS
                     TDGTPEYLETYARSWGLAQPRLRVMRQAHQGPAAARNLGAAAAQGDFLLFTDADCVPH
                     VDWIKEMVAPFALSAIAAVKGAYKTKQRSLVARFAQAEFEARYRQLAATEYVDVVFSY
                     SAGFRREVFRSIGGFDTSFPVADNEDTDLSYRVATAGYKIKFNPAAIIYHQHPATLKQ
                     YLRKKHSRAYWRVMVYKRFPGKAIRDSYTPQTLKLQIGSIVLAAGAIAFVPFVPHAMY
                     VAALAGGLFTATALPFLWQLPREDPGLRLVAPFLLVCRAAVMASGLMRTVPLLMSKTN
                     "
     gene            17525..18127
                     /locus_tag="PK589_13025"
     CDS             17525..18127
                     /locus_tag="PK589_13025"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNJ20575.1"
                     /translation="MRRILHTSWAVCFMVLCLVTTVQASETEAVLTPSVDAGWSGPFR
                     IGAGDVLNVFIWKHKELSTIVTVRPDGKINYPLIGEIEAKGLTLGEIEERINKQLKQH
                     IQDPQVTVILEATHSFRIFVLGEVMQPGVFDLKGPVTVIQALAMARGLTTFASRNKIF
                     IVNPARGNDQRIPFSYSKFVQGEDTNQNVMLRPGDTVIVP"
     gene            18320..19522
                     /locus_tag="PK589_13030"
     CDS             18320..19522
                     /locus_tag="PK589_13030"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ20576.1"
                     /translation="MTSPMLFAQESDNSEFVRPQREFGELPSWQNQVSYDRMPASEKL
                     LLGAPIRRDGFTPRLTVTEEYTDNVFFTAKNRGTDYITKIAPGLGYRTSGRDGQLSAD
                     YSVESRVYANNSNQNRTVSRQNGELFGLWNLSDRTSLTLFDRFESFQDPTEQQVPGIL
                     GAFGRTSINIGALMMRHRLTPSVDLLANYGNFLWSVDAPGAINSMTHEGEVGARVRET
                     QWGRTTVKYRFRFFDFQQGRDFQSHSALVAQEIDLSETLVVSGTIGAVRVEPNPSTVE
                     VLAQASIKKSIGDALYELSYMRDVFPPSGGLSQPLVGDFVRATAKIRLANNFLFDAGL
                     TWILTSTNSDDLTVHTLKWNVGISYTPASWLVARLGYDMFDQREDILGTSASRLANKV
                     SLRMIATF"
     gene            19594..20745
                     /locus_tag="PK589_13035"
     CDS             19594..20745
                     /locus_tag="PK589_13035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ20577.1"
                     /translation="MEPVSDGLRILFIAPAPRAGTVQYTHNLANALVDRGHRVTLVTG
                     VGFELADYPRKYTALEVFDRYRPRPLRLCRFVWHCLTFRPQIIHLQGAQHPALYILLW
                     AMLRLLGSASFVYTPQDVLPNSLRSYHIRAFRFLYARMRHVFLNAKQNEPLVIEHFAV
                     PRDRITVLPIADLTAFVREHVAGESPDIPAASRVVLCFGLIEPRKGIHTLLSAAPEVL
                     RQVPDALLLIVGKPLMDIAPLQRQLADLGLQGRVRLVPQYVSFAQMAGYFTAARLLVL
                     PYENGWNSGVLASAFGFGKPVIATRVGGFDEVVSDESTGLLVPPKDPAVLAQAIVRLL
                     RDDALYARMVVNVRQAAAEISWETIARMTEARYGDVVGIGADCAVSQAR"
     gene            20723..21589
                     /locus_tag="PK589_13040"
     CDS             20723..21589
                     /locus_tag="PK589_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNJ20578.1"
                     /translation="MQYRKLGNTGLTPSVLGFGCSMIASLATRYSPSEVEASLREARE
                     AGITFFDTADVYGQGDSERLLGKLFRRGGEGMILCTKAGLTVGSMEPVVRVVKPVMNS
                     ILRRWRTARVATTQARRRQEQQCFDPDYLSGRIEGSLRRLSVDRIDLFLLHNPPVDLP
                     QREGVFELLTRFKAQGKLRWFGVSCRSLDDAYEWLGQPDVACVQIPVDRSRIEAAIPV
                     LERARAQQVGVIAREVFAQDALAKGSVSDALGPLVERAEIGVVLAGMSCRRHVQENVR
                     ALDVAMRCSHVA"
     gene            21579..23237
                     /locus_tag="PK589_13045"
     CDS             21579..23237
                     /locus_tag="PK589_13045"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNJ20579.1"
                     /translation="MLLDARSISNGAILTADVCIIGAGAAGITLAMELLATGLNIILL
                     EAGGKSKAGESQSLYQGELNDPVRHLPLDQARYRQLGGTTSLWGGRCIPFDSLDFDQR
                     EWISHSGWPFPQKEFDGYYRRAHSYCECGEYDYRVSTALPGAPPSMLPGFEDGDVNTS
                     GIERWSPPTQFGKVYRPVLTRADNLRVLLHALAVELQTSSDGKRIDSVDVATFSGPRF
                     TVRARTTVLAGGGLETTRLLLASRGVHREGIGNHSDWLGRGYMSHIHGVIASVTLTAG
                     QDVMFGYEADPQGVFCRRRIAFSEEAQRRHRLLNLYMLLDRPLVGDPGHGNAVLSAAF
                     LLKRLLGGRQQEELGKGKYSLYWRHIKNILAGSPQALSVLPKFGRKRLLQRRRIPSLL
                     MRSRSNTYYLYFQSEQVPHWENRVTLDAARDEFGTPRLRLHFEVMEQDRQSVQRAHEL
                     IDQELRRQNCGYVTYLGNDVSQLMRDVKAVLGHHIGTTRMSADPSQGVVDAQCRVHGV
                     ANLFVASSSVFPTSSHANPTLTIVAMALRLADHLKALHSGVTRV"
     gene            23268..24278
                     /locus_tag="PK589_13050"
     CDS             23268..24278
                     /locus_tag="PK589_13050"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNJ20580.1"
                     /translation="MYWKNKRVLVTGGAGQIGSHLVARLAAAGAQVTVADNLWRGKKS
                     NLLGDDGAPVIDMESRFLELDLCDYRNCERATEGQDIVYHLADVVAGINYVFGNQFSL
                     FNTNLIIDSNMLRAAIANKVSSYVYVGTACSYPAERQSKLNPPPFKEDDVYPASPESS
                     YGWSKLMGEMGCDLARKEKLLDIGILRFHNVYGPHCEMSPEKSQVIPSLIRKAVRHPD
                     EEFVVWGSGKQRRAFVFVSDIVDALVSVATKGLNQGVIQIGPDYSTSIKEIAELVCAI
                     SGKPIDIRFDTSKPEGDVDRAADWTRARDILGWSPKTSIQEGLERTYAWCDRQVFQGV
                     GR"
     gene            24384..25391
                     /gene="gmd"
                     /locus_tag="PK589_13055"
     CDS             24384..25391
                     /gene="gmd"
                     /locus_tag="PK589_13055"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNJ20581.1"
                     /translation="MKKALITGISGQDGSYLAELLLAKGYEVHGIIRRSSSFNTGRID
                     AIYQDPHVAEPRLRLVYGDLNDASSLNKILRTIQPDEIYNLGAQSHVRVSFDVPEYTA
                     EVTALGTVRLLEAIRESGIQPKFYQASSSEMFGKVQEIPQRESTPFYPRSPYGAAKVY
                     AHWITVNYREAYNLFACSGILFNHESPRRGETFVTRKITRAAARIKLGVQQELYLGNL
                     DAKRDWGFAGDYVQAMWMMLQADQPDDYVIATGETHTVREFLDRTFSYLDLDWKQYVK
                     IDPRYYRPTEVDLLIGDATKAKKVLGWEPKVSFEDLITMMVEGDLRAERSILDGTRGA
                     R"
     gene            25394..26353
                     /locus_tag="PK589_13060"
     CDS             25394..26353
                     /locus_tag="PK589_13060"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNJ20582.1"
                     /translation="MSFWKDKRVVVTGGAGFLGTVVVDQLRKLGCRNIVVPRSHDYDL
                     VDMGDVKRLYVDAAPDLVLHLAARVGGIGANQTHAGQFFYDNLMMGTQLIEVGRQQGL
                     KKFVAVGTICSYPKYTPVPFREEDLWSGYPEETNAPYGLAKKMMLVQAQAYRQQYGFN
                     AVVLFPVNLYGPGDNFDMQTSHVIPALIRKCVEAKERGDDRVVLWGDGSPSREFLYVT
                     DAARAILLAAEHYNGNEPVNLGTGQEITIQALAHVIADAVGFTGEIVWDTTKPNGQPR
                     RCLEVSRAKQLFGFEASTAFVDGIQQTVAWFYANRGSIREVVF"
     gene            complement(26373..27764)
                     /locus_tag="PK589_13065"
     CDS             complement(26373..27764)
                     /locus_tag="PK589_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20583.1"
                     /translation="MKIHYAAVLALSFIAGIWISFDASTAEAQSRFSRKRQVQTQSVT
                     PPTTQTTAARDYSILKIVPPPAGGLYMGQYEWVQNDIASFESASGRKTALWSKYRGSW
                     GNGYDAAGQPHLDVQAANLAWQEGKVIVVQAYNTHPAPGESEAPQGFTVDKLLNGVYD
                     AELRRFAAELRQYGKPVFFIAGREPNGIGADYFGGFGPAGDQSLRWAIENKRGFSEFN
                     PSSLPYASLYSDIGTPQVCDGTERLKAAQRYYYDFFVRREGLKFLTFDTMGWSVNQVN
                     QIEYDVNELPSTIDKAYARQLLESCHAFSNFYAGDQYIDWISLNFYMIDYYAKDWPGM
                     TQDYVIPIENHFAALDKVMQEVKTVAPTKPVFFMELGFPDGMSQNSSWAAQKIATGIP
                     RIISGYPQIHGFAMWSAHPSWLIPGVFPYDCLIRPGTQQGTALKNVVTTTPSAFLSCV
                     YLSDGKLHPNCMN"
     gene            complement(27971..28339)
                     /locus_tag="PK589_13070"
     CDS             complement(27971..28339)
                     /locus_tag="PK589_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20584.1"
                     /translation="MMTRSWTFGLMAILIGITACSHFQNREAAYLDSVKGRATQTEVR
                     QELGAPKAVRKSDEGETVWVYEIREQQAGNRYTAPGIWCEEYLLTFDGAAVLQKWKQV
                     SHFHGGELMPTECIPGMEKP"
BASE COUNT         5946 a         7134 c         8635 g         6885 t
ORIGIN      
        1 tgttctagcc ccacccttca ccaaccaagg gtggggcgta acgaaggagg cggcatgggg
       61 gctctcgcag cgggcaggcc tggagggacg gaggcgcgga aggactgtcc cactgctcct
      121 aggacccggg tcgcaccaac aagggctttt cagcagtaag cgaacatggg caatttatag
      181 agaacgggat agttggctgg caggcatctt taacaaatgc ttcttgggag ttaacatgtg
      241 caggcttttg acaatggatg aggcatcgga atatttaggc atttccaagc tgacgttgta
      301 tggatgggtc tcagcaagga agatccggtt cgttaaaatt ggacgacttg taaagttcaa
      361 gcagcaagat ttagatcatt ggatagatgg acatacggtt atggctcgat cggaatccag
      421 aaaacctgaa gtcgaccgat gtgaacggtg aagcgaagct cggacttaca aaattgaagt
      481 gagagttgtt aatgttgaat ggttcgacaa atatccgtcg aaccagtgac cctcaagcgg
      541 cgttcgtgaa gttgaaggaa aggttttagt atggggctga cgaagcgaaa agattcatac
      601 tacgtggcat tcagtgtcat tgtttccgat gacgggaaga ccttcaagtt agctccaggc
      661 gtaccggggg caagactcaa gcgatggaaa gtcggatgtc ttaataaaac tatcgcaaag
      721 gagatggagg caaccatccg gatcaacctt cttcaaggta agattcttac agaagagtcc
      781 aggccagtgc tcttcagtga atgggcggag cagtatttgg accttgagga agtgcggagg
      841 ctgcgatcgt ttagcactcg tgccattcat gtcagccacc ttttgaagtt cttccgtgat
      901 aaaccgctat cagcattcac atgtgaagat gtcaggaagt acagatcctg gagagcaggg
      961 cagggtgttt ctgttcaaac gatcaatcac gatcatatga cgttgacccg gatgctgaat
     1021 cttgcaatgt ccgatcaatt caatctgatc tcgcgtaatg tttcagcttc agttccgaag
     1081 cccgatccta agaacgagcg agatcggatc gtcactcctg aagaatgggc agctattcgt
     1141 gcgcacgcag cgccacattt ggcgaggttt ctcaccgttg cttacgattt agggcctcga
     1201 cgtggagagc tactcaaact cgaatggtca gacgttgaca tgaagaggag ggagttcacg
     1261 cttcgagaaa caaaaaatgg agaggcgaga acggttccaa tgacgccaga agtttacgag
     1321 gcgtttcgcc agtgtttaaa ggagcggaga cttgatacct cacgagtgtt tctttacaag
     1381 gggcggccaa ttcagcgccg acttaccacc gcgttcaaag ctgcatgcag aagggcgggt
     1441 attgtgtacg gccgaaaaaa cggtggaatt actgcccatg acttccggca ttctgcagcg
     1501 acttccctgc gaagagcagg tgtggacacg atgacggcaa tgaaaatcat cggtcacaag
     1561 tcagaaaaga tgcacagaag gtacaatcag atttctccag cagatcttca gcgagccgtc
     1621 gataagctag tgatctacca ggctgatcag gcatctggag ctgacacggt attgacacat
     1681 gaaaagttgc accacgaagc gtaatcatgt aatatgttga aattcatcac ggggcgggcg
     1741 tagctcagtg gtagagtcct tgcttcccaa gcaagttgtc gtgggttcaa atcccatcgc
     1801 ccgctccaaa aaaccttttc aattccttcg taacacagac cttcaaggca cgtttctttg
     1861 tcgtcatccg tggggagtcc tctgagctcg tccaggggga gcatgtttcg ctcaagggat
     1921 ctggaattga aggcgcacac tggtcatgcc cttctgttgt gtcttaccca aggcaacgaa
     1981 ctgaaggcat ctggaggtgt ttggactgct tagtctgtat ggttccttgt gcgggattgt
     2041 atgctcgggg caagccgtga atatgttcgt ttgtggaggg gggaagctgt gacggcgtgc
     2101 tctttaggcc aaggcgggcg gtctggttag tccgttctgg tgtgcacgga ggagcagttt
     2161 ctgtcgattc gagacgccaa gcttgtcaaa aatgctcgtg agatgatggc gaaccgtgat
     2221 gctcgagatg cataggcggt tagcgatgtc tttgttggac agcccctcac ttacgaggcg
     2281 cacgacttcc tgttcacgtt cagtgagtcc gtccggccac ttggactgcg ctggatttgt
     2341 agcgattgct gtatgaatag tagggcggtg aggcttattc aaaggtgccg tgttgctacc
     2401 ggtaaatgtt acaggtgctg ctgccgctcg agcaaggtag tcaatcgtgg cgatcaaaac
     2461 gggagaaggc tgaatttttg gcactaggcc atcgactccg caggcaaaag cttgtcgtgt
     2521 gcactccgct tggtccattc cggtcaagag aatagttttg gtctctggag ctgttgcttt
     2581 gatcttttga atgagccccg gaacatcgct ctcgatctcc gtatcgataa tcattatttg
     2641 gggccgctcg cgcgcaatca cgtcgtcgaa tgttgttaca ttggcggttt gcccgatgag
     2701 ccggagccac ttttcctgtt ctacaattct ttgcaagccg agccgcaaga gataattatt
     2761 gctgacaatg gccaccaccg tggtagctcg ttcttcttct tcttcttctt cttttctact
     2821 catcaactct cctgactgaa actcacccac tggaacctcc acatgcaagg agtacttagt
     2881 cgcagtttgt gaaaatacta cgcagtgagc gctaggttta aaatgtgatg gagcgatcct
     2941 gcatgggcgc ataatctcac accagttatg cgccaatcaa ttgggaaatt ctaaccagca
     3001 atcctggcaa aaccttacgg ggaggcgctt tccggcgatc ctgaacgagg ggatgaacag
     3061 gataatctgg tgcacataag tgtgcactct catcctacga gtccacatac tcgtgtgttc
     3121 cctaggcagt ctaagccgga cgttacgtgc atagccgcgg aatgccaaag gaattattgc
     3181 tggcattttt accgcaccac atccttggac gcttatcggc actttgatcg gcacaaaagt
     3241 acgggtatca cacgtatcgt ttcgtaaagc gaaaggcacc aatgtccgat gtgtggtgca
     3301 tcctcacccc tgtatacgtg tctcccgcag gtagcactag gttgggcggc ttcggggaag
     3361 gcgcgttgaa gaaaatgttt agcctggtct tgccctcgcc gatcaatttt cgacgatagc
     3421 gccacatcgg acaatacatt ttcccggata ccgttgacgg atgatttctc gattcacgga
     3481 agagaagaaa caacaaatcc tgaaggaggg gcgctccaaa ggggggatcc agcatgtatg
     3541 ccgaaaatac ggcatttccg tcagcacgtt ttatcgatgg agagcgaaac agggttcccg
     3601 agtggtagat cccgccctcc atctgaggtc tttgcaaatc gagaaccgac gactgaagca
     3661 tcgagtcgcc gagctgtcat tagattacaa cattcttcga tcagcactaa tcagcgatcg
     3721 tggaagtgaa tgttaactcg tgccgaatct ccgatggcta ttggtttacg gctatgaaat
     3781 cgtcaaggaa tgacggtcgg agaacagtgc ggcatttggc ccaggtttta tttcggcgga
     3841 ttcctttgca aggctcggtc gcaatcgcgc cattgagatt gagtatggag caaatcacat
     3901 gaaagccgta atcatggacg gtgctccgtg ggcaggaacc atgctggcgt ttcctgtcgg
     3961 aaacgaattg ctaattgatc atttgctctc agcggtaaag cggctgggca ttcgcgaggt
     4021 cgccgtcgtg tcctgtgacc aagattccta cgttcgtgac ttggtgtgcc gcgccgggca
     4081 ttcgtatggc ctgacggtgt tgtggcgtac tgagcaagtc tatcggggca ctgcgggtag
     4141 tttgcaggcg gttgaagact ttctcgatac gccgacgttt ctaggcattc acgctaatgt
     4201 ctatttgcgc gacgtggatt tggcggccgc attcaaggcg catgatgagc atcgcgcaga
     4261 tatcacgctt gtcactcagc ggatggccgg tactgatgac gatctcgaaa gtgtggaggt
     4321 tgatcctgcg ggccttgttt ctagggtcaa tattttgcat tggtcgagga atcgtcggag
     4381 ccagttgacc ccttgtggtt tgtacgtctt cagccgggag gtcctatcac agattccccg
     4441 cgatgagtat tttgatgtca atgagcaact cgtgccgctg atgcgaaacc gtggaggtgt
     4501 gattcgtgct catcagatcg agggcggcgt acataccatt gtcgggcctg acgactttct
     4561 ttggctaaac cgcgagatct tgcacaagga tatacagggc agtgggcatg ggaacggtcg
     4621 gctcggtggg gtggaggata ttgtgattgg ggaaaattcc gagatttccc cccactcatt
     4681 tctgcttggc ccgctggtga tcggccccaa ttgtgtggtg gaagactatg tgcatttgat
     4741 tggcccagct gtgatcggtc caacctcgta tttggaaaag ggaagtctcg tgcgagagag
     4801 cgtcctcagg tcggggacaa gggtgcaagg gaatgccagg gtcgagtact ccgtgattgc
     4861 cggagacgag acagttcctg agggcatgcg tctgcgaagt gcgttttgga gcaccgcgaa
     4921 gccggctctc tatgagccgc atggagtccc cgcgcaggtc tcctcgaaca gaatgctaca
     4981 acgatcaagg tattctcaac ggcctgcaag aaggatgcag ggcggaaccg ggcaacggtc
     5041 gatctacggc atggtcaagt ccgtagtcga tgtcaccggg gccgcagttg gactactctt
     5101 cgcgggcccc ctgatgcttc tcattgcgtg tgcggtcaaa ttggattctc ccggtcccgt
     5161 atttttctct caaaagcgct gcgggaagaa tggcaaggag ttctggatgc ataaattcag
     5221 aaccatggtg ccggatgcgg agcaacgcca gaaagacttg aaggcccaaa atagtgtcga
     5281 tgggccgatg ttcaagttgc aagaagaccc acgtattacc cgcatgggca agcttctacg
     5341 taagacgagc ctcgatgagc tgccgcagtt gttgaatgtc ttgcgcgggg agatgagtct
     5401 cgttgggcct cgccctttgg cgtacgacga aatgaagttc tgtccgacct ggcgcgatgc
     5461 aaggctcagt gtggcccccg ggctaacggg tctttggcag gtgaaggctc gcaatcgtaa
     5521 tcgattctcg gagtggattc gatacgacct ggagtatgtg cgtacgcatt cgttgtcgct
     5581 ggatctgtat attctcatcc ggaccgctcg ggtgttgctg aaaggagtgt gatgaacgtg
     5641 ccgtacacga ctgggctccc cgacgtagac tctacgcaga ctgtcggtcg atcgtcattc
     5701 ggcgtccgag agcacctcat cacgatcttc cgccaaaagc ggaagttact tacaattttg
     5761 ggaatggcaa cccttgtggc ggggccgggc tcattcttga tgacaaacat ctatagtgcc
     5821 gaggttcgat tgctcatcca gaatgcgcga accccgttca gcatgagcac gccgttgacg
     5881 gggcaggtgt tcactccttc cgatattact caaaaagatg acgtcgcgac ggaagtgcag
     5941 attttcacta gcccgattct gttggataag ctggttgact acttcggaaa tgaacgagtg
     6001 cttgctgcca tgcgagggcg gtgggactgg gtcggtgaaa tcccgaaaac cgttttcaaa
     6061 cagttgtccg ggttgccgcc ggtggccaag ttattgacga cgatcgggta tgttcccaag
     6121 ccggaggatc tgcactatca agccgtgcag aaaattcggg cgcatttgaa tgtggaagga
     6181 gtgcggcaaa cgcacgtatt tgtggccagc ctcgattccc ctgatccaga gttttccgca
     6241 gatgcgctca atgcgctggt cggcatctat atggatcacc agctatcagt ccgcaaaggc
     6301 aagggtgcgc gcgaattctt tgacgagcag acgaagcaga tgcgcggcga gttacagcag
     6361 gcagaactcc ggttacaggc ctttaaagac aaatggaaca ttgtctccat cgaagatcaa
     6421 aagcggcatc tcctgcaaca agtgacgcac accgaagccg cacttcgtga gagtcaggtg
     6481 caggtcgcgg aaacggaagt gcgcatcagc aaactcaagg aacgcctggc tgggcaacag
     6541 gaagcgattc cgcttacgaa cgtctcggag cgaaatccca tgctggatca actcaagaac
     6601 cggttgatgc agatggagtt ggagtattcc cagtatgtcc ccgacagtcc ggctgccgca
     6661 gaacttgtgc gagaaattgc cgcggtgcgt gcgcgcctgc aggcggagag ttcaaaggtc
     6721 accggcgcag ccacatccgg cctgaatcaa aactatcaag aattgaaacg caccctggtc
     6781 atggaagagg ggcgtcgaga aagcttgcgg ccgcgccttt ccgagctgac caagcaattc
     6841 aaatcctacc gagattctct caataccctg gatcagcgag aaatggaatt gcagggactg
     6901 atgcgggagg tcaaggtcaa gcaggaagcg ttcgacatct accttaagaa ggaggaagag
     6961 tcgcggatca atgaagtgct tgatcgcaag gggatttcga acgtcaatgc aattgagcat
     7021 gcaacagctc cccagaagcc tgtacagcca cgtaaatttc tcaatctcgt gatcggtctg
     7081 ttgatcgggt tggtgggggg attcgggtcg gcctatgtat cggagtatat gcgtcgaacg
     7141 ttcgttaccc gcgaggaggc ggaagataac ctcgggcggc ctgtccttgc tgccttaccg
     7201 ctcgtccgtc cagacacgtc cgatgccggg agttttgact tggaactccg ccaagctgca
     7261 cagcacgtca ttcgttccta ccatgagcgt ggtctgagaa cattgcttgt cacgagcgcc
     7321 ctccgcggcg aaggccgatc acatgtggcc ggcgccctcg cgcgagtgct ggccgagcag
     7381 aagtttcgcg tgctgcttat caccttcgaa gaagccggcg cagagaagct aaacaccggg
     7441 gatgccgctg cgggggctaa gcaagcgacg gataccggcc ggttccggca ggatattcca
     7501 gaggcaaccg atcgtatcca cctccatcga ttactggttc gccacaggga aggaacggcg
     7561 ttggaatttg ccgagcatct ggcggaggtc gccaagacca tgcgcgatcg tttcgattta
     7621 atcgtgatcg acggccccgc attgacctcc tttcctgaaa tgcgcgtggc cattgcaggc
     7681 attgatggca ccattttggt ggtcgaggcg gagcggacgg tgtcggtggc ggcggccaga
     7741 acgacgacgg cgatcgaagc ggccggagga cacttgctgg gactcgtgtt gaacaagcgt
     7801 cggtacatta ttccggaatg gatttacggg cgatggttgg cggcaggcgg gagggagggc
     7861 gtatgagtga atcgatagcg ggtgtaatgc aagtcgatga gaaaatagtc cctgcggagg
     7921 ggttcgttcg agaagacggg tggtatgcgc cgtatgacta ttggctcacc ctctggggac
     7981 tctacgacct ctacgcctat gcgctggcat tgcttggtga cgtcaagggc aaggttctgc
     8041 tggattgcgg ctgcggtccc gggcacacgt ccgtgatgct ggcgaaacgc ggggcgatcg
     8101 tcaccgcgtt tgatacggcg gacggtcaac tcgatacggc gcgaaggctg gcacaggcca
     8161 acggtgtcaa tgtgacgttt ctttgcaagc ctttcgagga gttggatttt ccggacgaga
     8221 gtttcgacgt cgtcttcggc acattcgtct tgcaccatgt ggatcttccc aaggccagcc
     8281 agcaattggc tcgcgtcttg aagccggggg ggaaagcggt gttcatcgaa aactccgcgc
     8341 tcaatccgct gctcatggca gccagaagca aggtctgtgg acggttgggt gtgcctcaat
     8401 acagcgacga tcatgagcat ccgcttaccc gtcgcgacat tgcgacgcta cagagcgcct
     8461 ttcccggcgc ttgcaccatt cattttccga gtttcctctt ctttcgattg ctcgatttct
     8521 acgtcttcaa gaaacggtgg tcgtggatga cggccctgct tcgacactgc gatcaactcg
     8581 ccgggtcaat tccaatcgtg cgtcgatacg gctatctgca gatcgtcgag ctgggaaagg
     8641 gccaaggtcg tgcgtagcaa accgtccaaa ttcggacggg gattttgctg tgcattgttg
     8701 ctcctgtccg ggctcttctt ggtggcgacg gacgcccggt ctgctcctgc gaccgaggcg
     8761 gtggccggtt gtcggaccga ggcgcctgcg ttgaaacacc atcgtgcgtc cgctgtcgat
     8821 cgtgtcaccc tgacttcgcc ctcgaacggg gtttacatcg gcttgtatag catcggaacg
     8881 attccggaag accggttacg gtttactgcg aagagcgggc atcggcctgc cattgtgttc
     8941 acctttcatg attgggtctc cgacgacgat tggaccgcat cttcgccgca tttcaggact
     9001 ttcacggatc cgttggaatc gtcccggctc agtccgctcc agctggccga gcaggtcaga
     9061 agcgaggggg gtgtgctggc ggttgcctgg gccatccagt gttgcgattg ggagtcccac
     9121 ctgttctggt atggatttcg taaaccgacg gtgacggttg atcgcctgct tcgaggtgat
     9181 tttgattcat acatcaccag cgtagcccgt cagatcaagg cgtacaggca tccgatcatg
     9241 cttaccctat tcagcgaatt caactatcag gggatgatgg ggttcggtaa ggagggcaga
     9301 acgcgactcg atgatgtcga tcacgtgtgt cgactgtatg gagatccgac atggccggac
     9361 gggccggaac ggattcggga cgccttcatc tacgtcatcg atcggtttcg aaatgaagac
     9421 gtgcgaaacg tgacctggtt tatgtacgca gggtctcatt atatgaatcc ccagcatgag
     9481 gactattccc catggttaca cccacaatat ttctatcccg gcgacgacta catcgactgg
     9541 gtggggcaga gcgcgtattt catcgatacc cggtccgttc caaaacttcg acagcaggaa
     9601 ctcgctacgt ccttcgcagg ggcattgggg cctgggtatg ccgcgtgggg cgcagtgact
     9661 gatcggccgc tgtttctccc tgagttcgga gtgttgggag ataagaccag cagtcgtgcc
     9721 cgggtaatag aggaagtgtt ccgcgacgtg ttgccgcagt tccctcgagt gcgggcgatc
     9781 accctcgctg atttcaaaat tgccgaggat tactacgaag tgcccaggtt cggcaccttc
     9841 gaggatgaaa cggaagcctg gaagcgggcg gttcgtcaga accctcggta cctcaagcaa
     9901 gcctctttca aaggcggaga gtaggccggc aatggcgctg aagcgatctt cgcaatggct
     9961 gtgtctctgc gcgatttttg ccgtgtgttt cactctgcgt atcggcgtcg gcggggtcat
    10021 cgacgcgacg gtatcggttg tcaaacgggc ggatgtgtac tcggacattg cggtgaatct
    10081 ggcgcgcggc cacggttttg tcgttgtagc gggtggcgag cctataattt ggcgcgctcc
    10141 gttgtatccg gcatttttgg cgcaggtcta tgcgctcttc ggggagtaca gtgaaacagg
    10201 agtgtttctt gcgcagtccg cgttggattc gttcaccgcc gtcttgatct tttatatggg
    10261 cactcgcttg ttcagtgaaa ccgtcggatt gctctctgcc gtgagttttg ccctgcatcc
    10321 gctgtccgcg tattactcac ttcgattcct gtctgagccg ctgttcacct tggcctttac
    10381 agccctcatc tcggcttgga tcgcggcaac gactacccgc cgtctcatgg cctacacggt
    10441 cgtaggtgcg ttgatcgctg tcgcagcgct ggtgaagccg gtggctttgg gcttgtggcc
    10501 cttgcttgcc acctgcgccg tatggcagtt gcgggacgaa ccggttcgcg cactatctgc
    10561 tgtgaccggg ctgacccttg cttgtctgat ggtgttggct ccttgggctc ttcgaaacta
    10621 tcgcgcgacg ggcgaagtgg tggccgtggc aacgggggga ggctatgctc tctggcttgg
    10681 caatcaaatg gtaagtgagg gcaaggagga ctgggaagtt gatgaagtga cccgggcgca
    10741 tctgttcgaa cggcgtggca cggtcatcac aggggctcaa ccgtcggatc acgctctcgc
    10801 tggttatccg tcgatccgat cggcaacaca gccggttcac atttcggtcc ctgaagatca
    10861 tgcatttttc atagctgcct ggcgggaaat gagttcccat ccgttcgaca cggcactatt
    10921 gacggttcgg aaattctttc ggttctggtt caggattttt cttcctgaca atcgctgggc
    10981 acaatcctac attgtcgtgt ttcaaaccct cttcctgagc gtggcggttc tgggcatgct
    11041 ggaggcgaaa cggcagggaa tcacggtgtt tgcgttgatc ctgccggtcc tattcctcgc
    11101 ggccgttcat gcaatgacat tctccaccat tcgctacagc attccgacca ttccggtctt
    11161 gagcatcttg caggcggccg gcttgcgggc gatcgtgcat gcactgaatg agcgatgggg
    11221 tttgtcgctt ggagtgtcct gggtccgttg gttaaaggct gcgctggtcg gctcagcggc
    11281 aagatcgtac cgggggactg cgttatgacg gcgcctgcga tgctgaaaag ctgtgccgag
    11341 agtaggaacc gctgatgaaa ccgattcgag tcgtgatcat tcgcaactgc cgtgggatta
    11401 ccggcatgac gggaggagaa acctatcttc tttctctctt gaagggattc gacccacaga
    11461 aagttcgttg cctcctggta tgcatcgtca atcccgcact tggcgaaacg tcttggctta
    11521 gggaattgaa gcagaccggg tttgagcacg tgacgattcc gatcgggaat cccttcaacc
    11581 tgagcgatgt ctataaggtc tcttccttga tcagatcgta tgaggcagac gtcgtgcacg
    11641 ccctggacca ccgctcggat ctgataggga tactcagtgc gcgtctaacg ggacgcccgg
    11701 tgctggcctc gtttctcgga tgggtcaatt tccctaaagg gtcctggcgg gcgaccgttt
    11761 atccatggat cgatcggatg attctcaagc ggttggatgc agtgattacc gattcggtgg
    11821 cgattggcac agaattaaag atgcgtgagc aagatccgcc tgtggtcgcg attcgaaatg
    11881 gcatcgatac caggtggttc gatcccacgc gtacgctcac gccgttttct caggaaagct
    11941 ttctcaaggg tggccatttt gtgttcggca tggtgggccg tattcatccg gtgaaggggc
    12001 atctgaactt cttaaaagcg gcgcgaaccc tgcttgaaac gcacgcagat tgtcggtttc
    12061 tcatcgtcgg gacggtattg ccgggatttg aacactatcg gcagggcctc caggacttta
    12121 tccgcgaaca agggttggag caatcggtgt tgatcaccca cgtgtcattg gcggagatcc
    12181 ctgccgtgtt tgccggcatt gatgtgttgg ttgcgccgtc cttcgccgag agtttcagct
    12241 ttactctact cgaaggcatg gcaatggaaa aagccgtggt gagttcagag gtcggagggg
    12301 ccggtgaaat gatcactcac ggcgaaacag ggtatctgat accggttgat gacgtctccc
    12361 ggttgaccga aaccatgcgg atgctgaggg ccgccccgga aaaagtgcgg gaagtgggtc
    12421 tgcgtgcgcg caagaaaatt gtcaacgacc tggggatcga ggcgatggca gcgcgaacgc
    12481 atcaagtgta ccgcagtcta atcgagttta agggcaagac gtcgggcggt gcgggagacc
    12541 aaggtcgatt gcgcgagcag ctggccatga tcagcgccaa gtaacccatc gcaggaactc
    12601 atgatggacg tattgtgact atgaaaattc tgattaccgg ttctcagggg tttctcggca
    12661 agagtcttgc tcactatgcc gtcaggaacg gacatcatgt gttaggtgtg tcgcggtcgg
    12721 acgccgacgg tttgattgcc ggaatggaat ctatacgctg cggcctggac caggcaggct
    12781 ttgtcgacgt actcaatggt tttgctccgg atgtcgtggt ccatgcagcc ggttctgcgt
    12841 cggtgggata ttcgtttcaa cagccacgag aagattttct gatggcagtt gaaacctggg
    12901 cgtcggtctt ggatgcagtg cgcaaggcag agggaaaccc gttggtgatc tttccatcga
    12961 gcgcttccgt ctatgggaat ccgactcggc tgcctgtacc cgaaggtgcc gggcttcgac
    13021 caatttcgcc ctatggattt cataaggtca tttgcgagca gttggcgcaa gaatatgccc
    13081 actgcttcgg gttgaacgtg gtctcggctc ggttgttctc gaccatcggc ccatttcagc
    13141 agcgcttatt ggtctgggaa ctatttcgcc aagcgatcga tgccaacccg tacttgacca
    13201 ttcagggcac agggacggaa acccgtgatt ttctgcatat cgacgatatt tgtcggtatt
    13261 tcctgggtat cgcagagcgt gagccgaaag gattttgggc ggtgaacgtc gcttccggcg
    13321 agtctacgtc ggtgagaacg atggcggaaa tggtgacgcg ccttgtggga agtacccagg
    13381 ctatcgtcac tctgaataag gatttgccgg gtgatcccaa acaatggcag gccgatacaa
    13441 cgctgttgca agaactcgtg ccgtatcagg cgccggacat cgccaacggc ttggaggatt
    13501 gtgtgcggca ttggttgtcg gagccgtcgt tgtcggctca acgggagctg cgttgtagtg
    13561 tggaggggca ctcctgaacg ggcggtacgg gataaagatc acggtgccgt gacggctagc
    13621 tgctgccagg gtacggttgt gtgtgccggt tattcagtgc gaaggggatg gtgtaggcgg
    13681 acatgcaggt gttgcgttcg agtatgaagg cgttgtcgca gcagaccgcc gtggtgtttg
    13741 ccggaaaaat gaccggcgcc tgtctggggt ttgtgatgag cctgcttgtc gcgcgttgga
    13801 tgggaccgga ggagttcgga ttattttccc tgttcatcgt gatcctgatc ttcggcaatg
    13861 acgtgcttgg cgacgggttg agccctggag ttgtgcggtt ttattccatg tatcggcgtg
    13921 ccgacccctc caaggcagct gaagtgctca ccaacgcgct tgcgttgcgc atgctgctcg
    13981 gcattccggt ggttgtggtc ggtgtagccg tgggatcgca atatgcggct agggtgtttc
    14041 atagccagtt gtacgtgctc cccgtagtct tgggtctagt cggctccttt ggtgcggcct
    14101 tgtggagttt caacctctcg gtgtggcagg cccgtgaaga gttcggtacc tatggggtga
    14161 tggtcgcgct gataaatgtg ctgagggtcc tcagtctacc gttgttgttc ctgggaggat
    14221 atctcacgct gggcggaatc atggggtccc atgttctcgt ctatttcctc tgtgcgctgt
    14281 cgggtacctg ggcactgcgc aggcatctgg cacatgttcg cgttaacggc gaattgttgc
    14341 gggaactgtt tcgattcagc aagtggccgg cgatggccag tctctgtttt atcctccaag
    14401 tgaatctggg cgttccggtc cttaactatt ttgtgaatgc ccgtgaagcc ggcctgtatg
    14461 gtgccggatc atcactcttg atgggagtgg attttctgac agtcagcctg ctgacgacat
    14521 tgcttcccaa ggtcagtcag ctgaccggga ttgaacagtg tcgttcctat gttcagcgct
    14581 catttccgat gtatgcactc attgccgctg caattcttcc gttgctgttt ttcgcacggc
    14641 cggtggtcat cggattgttc ggttctgcct atgaaggcac gatcgaggtc tttcaggttc
    14701 tgtttgtcgg aacgttagga accttggtga cgcatccttt gtatctagtg ctctatacga
    14761 tgaatcgccc gcacctgcac acattgtcag gaattgtagg actgattgtc tggctgctag
    14821 ccggcttctg gcttattccg caattcggtg ccgtcggagc cgcgtggacg acgttgtgct
    14881 cacgacttgt tcaatccctc atgatcgttg tcatcctttg gcatgccctg ggattgggcg
    14941 gatcgtcggg cttggcgcgt tcggttatgc cggatgtgag aggatcatga aaccgagcga
    15001 tcagacgtat accattccga ctctgctggg cgcgacgctg ggcgcatccg tcttggcgct
    15061 ctcgaccaaa gctcttgcta ttgtcattgc ctgcagtgcg tttctcagta tgaccgcctc
    15121 ggcagttttt ccttattaca tactggttgc caccattcct gtacaggttg atttactggg
    15181 aggactgacg gtcacaaagc tgattacccc gttggctata ggtatggtga ccatcaatgc
    15241 gctgatccat cgaggaccat ggccggtgct gttacgatgg ccggccggtt atcttgccgg
    15301 cgcgttcttt cttgcctctc tcttgtctct cgtcctcgcg gagggcttac gtggactgcc
    15361 aggcgaggca gcaaagatcc ctgtttacgg ggcgctcttc tttttcacac ttacatttaa
    15421 tcgccatcca gaggatatgc gccgtctgct gtgggtcatc accattaccg gtgtggggga
    15481 ggcactcatt acagcggctc aggtccacta cggttttgtg atgccgggcg actggcggag
    15541 aaacgtaggc ctgccaagcg aaggcggtgt agacggcgcg ttcatgtcta tttccgaggg
    15601 aaaggttcgt gctgagggta ccaccgccca tcccattttt ctcgcaagtt tcttcctgat
    15661 gtccatgccc tgcaccgcat tccttctttt tcaggagaaa catccatggg tcaaggccgc
    15721 gctgggggcg atgctgagcc tcatggcata tgcgtggttt tacacgtttg ctagaagttc
    15781 ggttatcggt ttcgccctcc tgattctggt tggtctgtgg ttgcaattca aattcgtgag
    15841 aaagctgtct cttattgtgc tggtgctcgt gctcgccgga ggcttgagtt atcaggttgt
    15901 ctcggaatgg tttcaagtcg gagtgcaaac cgtagagaat caaaacctct tcggtaaggc
    15961 agatctcaac gaaggcagcg ggagttttgc atttcgacgg gagtccattg tgggtggctg
    16021 gaatctcttt ttggcgcacc cttggtttgg tgtcggcttc ggacaggcca tcaatcatta
    16081 cgtcaaatat ttgccagcat gggccgacca ctactatcac cctgctgtga tccacaatat
    16141 gttccttgaa gtggcgagtg agcttggcgt gttttcattg tcggcattca ttggtttgtg
    16201 ggtggtggcg ttcgtcagtc tgaagcgggg gcttcggatt cccgatgtgc gaccctacgc
    16261 cgttctgttg agcagcatcc tcgcggggca aatgattttt ctgatgatca ccccgatggt
    16321 gcgagagatt tggctgacat tgccgatggc catcgccatc ggacatatgg ccaagaccca
    16381 cacgcgggcc acgttcggca taccctttcg acaggtgggc gctcgtgctc aactacgacg
    16441 gatcgttgat cgtgggaatg gcctgcctgc aggcctgagg tgaggacatc atgaaaattc
    16501 gcgtcagtgt cgtcattcct gcctacaatg ccacgaaaac catcggcgat ggcctggagt
    16561 cctttgctcg tcagtcattt ccagcagccg aagttgagat catcattgtc gatgatggct
    16621 cgacggacgg cacgccggaa tatctggaaa cctatgccag gagctgggga ttagcgcagc
    16681 ctcgattgcg tgtgatgcga caggctcatc aaggcccggc ggcagcgagg aaccttgggg
    16741 cggcggcggc acaaggggat tttctgttgt ttacggatgc cgattgcgtg ccgcatgtgg
    16801 attggatcaa ggaaatggtg gcgcccttcg cattgtcggc gattgccgcg gtgaaggggg
    16861 cctacaaaac aaaacaacgc agtctcgtgg cgcgctttgc ccaggccgag tttgaggccc
    16921 gctatcgtca gctcgctgcg acggaatatg tcgatgtggt cttttcctac tccgccgggt
    16981 ttcgtcgtga ggtgttccgc tctatcggag gattcgacac gagtttcccc gtcgccgata
    17041 acgaagatac cgatctgtcg tatcgggtcg ccacggccgg ctacaagatc aagttcaatc
    17101 cggcggcgat catctatcat caacatccgg cgacgctcaa acagtatctc cggaaaaaac
    17161 atagccgggc gtattggcga gtgatggtgt acaagcggtt cccgggcaag gccattcgtg
    17221 attcctacac gccgcaaacg ctcaagcttc agatcggctc gatcgtgctg gctgcggggg
    17281 cgatcgcgtt tgtaccattt gtgcctcatg ccatgtatgt ggcggcactt gcgggcggac
    17341 tcttcacagc cacagcgttg ccgtttctat ggcagttgcc tcgagaggat ccgggattac
    17401 gactggtcgc gccatttctt ctagtgtgtc gggcggctgt catggcttca ggtttgatgc
    17461 gaacagtgcc gttactcatg agcaagacca attgagactc atgttactga gggggagaca
    17521 agacatgcga cgtattttgc acacaagctg ggctgtttgt ttcatggttc tgtgtctggt
    17581 gaccactgtg caggcatccg agaccgaggc ggtacttact ccgtctgtgg atgcgggctg
    17641 gagtggtccg tttcgaatag gtgcggggga tgtcctcaat gtattcatct ggaagcacaa
    17701 agaactgtcg actattgtca ccgttcgtcc cgacggaaaa atcaattatc cattgatcgg
    17761 cgagattgag gccaaaggac tcacgctggg tgagatcgag gaacggatca ataagcaatt
    17821 aaagcagcac atccaggatc cacaggttac ggtgatcttg gaggcgacgc atagtttccg
    17881 catctttgtc ctgggtgaag tgatgcagcc gggtgtcttt gatctcaagg gaccggtcac
    17941 cgtcattcaa gcgctggcca tggcacgggg attgaccacg tttgcgtcgc ggaacaagat
    18001 ttttatcgtc aatcctgcgc gcggtaacga ccaacgcatt ccgtttagtt acagcaagtt
    18061 tgttcagggg gaagatacga atcagaatgt gatgcttcgg cccggggaca cggtgatcgt
    18121 tccgtagtgt cagtggaatt tcaacaatcc gggacgaaag cgaagttgag tgtcacacag
    18181 aatggaatgc cttgtcacat ccgggagtcg cgtgagcagg cgaggtatcg ttttgaacag
    18241 tgcacgaggt ccgattgggc ggactttccg ctggttgcgt attgcggtgc ttggaattgc
    18301 atcggctgca ggtgcagtag tgacctcgcc gatgctcttt gcgcaggagt cggacaactc
    18361 ggagtttgtc cggccacagc gcgagttcgg tgaattgcca tcctggcaga atcaggtgag
    18421 ttatgaccgg atgccggcgt cggaaaaact gctcctaggt gctccgattc gccgagatgg
    18481 gtttacgcct cggctcacgg tcactgaaga atatacggac aacgtcttct tcacggcgaa
    18541 gaatcgcggg accgactaca ttacgaagat tgcgcctgga ctgggttacc ggacgtctgg
    18601 gcgagacggg caactctctg cggattattc ggtcgagtcc agggtctatg cgaacaacag
    18661 caatcagaac agaacggtgt cgcggcagaa tggggagctg ttcggacttt ggaatttatc
    18721 cgatcgaacg tcgctgacct tgtttgatcg ctttgaatca ttccaagacc ccacggaaca
    18781 gcaggtgccc ggtattcttg gcgcatttgg gcgcacctcg atcaatatcg gggctttgat
    18841 gatgaggcat cgactcactc cgtccgttga tctgctagcg aattatggaa attttctctg
    18901 gtctgtcgat gcgccgggtg ccatcaatag tatgacacac gaaggcgagg tgggcgcgag
    18961 agtacgggag acgcagtggg ggcgtaccac cgtaaagtat cggtttcgat tctttgattt
    19021 ccagcagggg cgtgattttc agtcccattc tgcgctggtt gcccaggaaa tcgacctgtc
    19081 tgagacgctc gttgtgagcg ggacgatcgg tgcggttcgt gtggagccga atccttcgac
    19141 ggtcgaagtc ctcgcgcagg cgtcgatcaa gaagtcgatc ggggacgcgt tgtatgaact
    19201 gagttatatg cgcgatgtgt ttcctccatc gggaggactc agtcaaccgc tggtcggaga
    19261 ttttgtacgg gcgacggcaa agatacgact tgcgaataac tttctcttcg atgccggact
    19321 gacgtggatt ttgacgagca ccaattcaga tgatttgacg gtacacacct tgaaatggaa
    19381 cgttggcatt tcgtatacgc cggcctcctg gcttgtggcc cgactggggt acgacatgtt
    19441 cgaccagcgt gaagacattt tgggcacgtc cgccagccgc cttgcgaaca aggtcagtct
    19501 gcggatgatc gcgacattct gagggtgtgc atacgctacg gaattgatga catcgggtga
    19561 gactccgagt gcagagcagt aaggtgggtg aaaatggaac cagtgagtga cggacttcgc
    19621 attcttttta tcgcgccggc tccgcgcgcc gggacggtgc aatataccca caacctggcc
    19681 aatgcgctgg tggatcgggg gcatcgcgtc acgctggtca caggtgttgg attcgaattg
    19741 gccgactatc cgagaaagta tacagcgtta gaagtatttg atcggtatcg gcccaggccc
    19801 ttgcggctct gtcggtttgt ctggcattgc ctgactttcc ggccgcagat tattcatcta
    19861 caaggggcgc aacatccggc cttgtacatt ctgctgtggg cgatgttgcg gctcttgggc
    19921 agcgcgtcgt tcgtctatac gcctcaagac gtcttgccca attcattgcg ttcctatcat
    19981 atccgcgcgt ttcgttttct ctacgcccgc atgcggcatg tgtttctcaa tgccaaacag
    20041 aatgagccgt tggtcatcga gcattttgcg gtgccgcgtg accggatcac ggtgcttccg
    20101 attgccgatc tcacggcgtt tgtgcgggaa cacgtggccg gtgagtcgcc ggacattccg
    20161 gccgcgtcgc gggtggtgtt gtgcttcgga ttgatcgagc cgaggaaagg gatccatacg
    20221 cttctctctg ccgcgccgga ggtgcttcgg caggtgccgg atgctctgtt actgattgtc
    20281 gggaagccgc tgatggacat tgcgccgtta cagcgacagc tggccgattt ggggttgcag
    20341 ggacgggtgc gattggtgcc gcagtacgtg agttttgcgc agatggccgg gtatttcact
    20401 gccgccaggc tcttggtgct gccgtacgaa aacggatgga atagcggcgt cttggcgtct
    20461 gctttcggat tcgggaaacc cgtgattgcc acgcgagtag gcggattcga cgaggtcgtc
    20521 agcgacgaat cgacggggtt gctcgtgccc ccgaaggatc cggcagtgtt ggcgcaggcg
    20581 atcgttcgcc tgttgcgcga tgatgcgctg tatgcgcgca tggttgtgaa tgtccggcag
    20641 gcagccgcag aaatttcctg ggagacgatt gcacggatga ccgaagctcg gtacggggac
    20701 gtcgtgggga ttggagcgga ttgtgcagta tcgcaagctc ggtaataccg gtctgacgcc
    20761 gtcggtgctg ggattcggat gttcgatgat cgcttcgctg gcgacacggt attcgcccag
    20821 cgaagtggag gcgagtcttc gtgaggccag ggaggcgggg attacgttct ttgatacggc
    20881 cgacgtctat ggtcagggtg atagtgaacg tctgctcggg aagctgtttc ggcgcggggg
    20941 cgaaggaatg attctctgta ccaaggctgg gttgacggtc gggtcgatgg aacctgtggt
    21001 gcgagtggtc aagccggtga tgaattcgat cctgcggcgt tggcggactg cacgagttgc
    21061 gacgacgcag gcgcgccgcc gccaggagca gcagtgtttt gacccggact atctttccgg
    21121 acgaatcgag ggaagcttgc gccggctcag cgtggatcgg attgatctct ttctattgca
    21181 caatcctccg gttgatcttc cgcagcgtga aggagtgttt gagctgctga cccgcttcaa
    21241 ggcgcaaggc aaactgcgtt ggtttggcgt gtcctgccgg tcgttggacg acgcctacga
    21301 atggctaggg cagcccgatg tcgcctgcgt gcaaattccc gtcgaccgca gtcgaatcga
    21361 ggcagcaatc ccggtgttgg aacgcgcacg agcccaacag gtgggcgtga ttgcacggga
    21421 ggtattcgcg caggatgcac tggccaaagg ctccgtgtcc gatgcgttag gtccgctggt
    21481 ggaacgtgcg gagattggcg tcgtgctcgc gggtatgagt tgccggcggc atgtgcagga
    21541 aaatgtgcgt gcgctggacg tcgcgatgag gtgtagccat gttgcttgat gcgcgatcga
    21601 tttccaacgg agccatcctc accgctgatg tctgcatcat aggcgcaggt gccgctggta
    21661 ttaccctggc gatggaactg ctggccactg gtctgaacat tatcctattg gaagccggcg
    21721 ggaagtcaaa agccggagag agtcagagcc tctaccaggg agagttgaac gatccggtcc
    21781 gacacttgcc tttggatcag gctcggtatc gccaactcgg tggcacgaca tctctgtggg
    21841 ggggacgctg tattccgttc gattccctcg atttcgatca acgcgagtgg atctcgcaca
    21901 gcggctggcc gttcccgcag aaggagttcg acggctatta ccggcgtgcc cattcctact
    21961 gtgagtgtgg agagtacgat taccgtgtgt cgaccgcgct ccccggtgcg ccgccatcga
    22021 tgctgccggg gttcgaggac ggggatgtga atacgtcggg tatcgaacga tggagtccgc
    22081 cgacgcaatt tgggaaagtg tatcggccgg ttctcacacg tgcggacaat ctgcgcgtac
    22141 tgctgcacgc cctggcggtt gaattgcaga cctcatcgga cggtaagcgg atcgattcgg
    22201 tcgatgtggc gaccttcagc ggccctcgct ttaccgtacg tgcgcgcacc acggtgttgg
    22261 ccggcggcgg attggaaacg acgcgattgc tcttggcatc tcgaggtgtg catcgggagg
    22321 gaatcggcaa ccattcggat tggttgggac gcgggtacat gtcccatatt cacggcgtca
    22381 tcgcgagtgt cacgctcacg gcaggacagg atgtcatgtt cgggtacgag gccgatccgc
    22441 agggtgtttt ctgcagacgt cgcatcgcct tctcggaaga ggcccagcgt cgacaccgtc
    22501 tcttgaatct gtacatgctg ttggatcggc ccttggtggg ggacccgggc cacggcaatg
    22561 cggtcctttc cgcagcgttt ttgctcaaac gtctgttggg agggaggcag caggaggagt
    22621 tagggaaggg caagtactca ctctattggc ggcatatcaa gaatatcctg gcgggctccc
    22681 cccaggcatt gtcggtactc ccaaagttcg gtcgaaagcg gctgctccag cgtcgacgga
    22741 ttccttcgct gctcatgcgg tcccgctcaa acacctatta cctctacttc cagagtgaac
    22801 aggtgcctca ttgggaaaac cgtgtgactt tggatgctgc gcgagatgag ttcggcacgc
    22861 cgcgattacg gcttcatttc gaagtcatgg agcaggatcg gcagagtgtg cagcgggcgc
    22921 atgagctcat tgatcaggaa ttgcggcggc agaattgcgg gtatgtgacc tatttgggaa
    22981 acgatgtgtc gcaattgatg cgcgacgtga aggcggtgct gggtcatcac atcggaacca
    23041 cgcgtatgtc ggcagatcct tcgcagggtg tggtcgacgc gcagtgccgt gtgcatgggg
    23101 tagcgaatct gtttgtcgcc agcagttcgg tatttccgac atccagccac gccaatccga
    23161 cgttgacaat cgttgccatg gcgctgcggc tcgccgatca tctcaaggca ttacattccg
    23221 gcgtcacgcg agtttgagac agtatttctg tgggaaaggg gaacattatg tattggaaaa
    23281 acaaacgtgt gcttgtcacc gggggcgcgg gtcagatcgg gtcccatctg gttgctcggc
    23341 tcgctgctgc gggcgcacag gtaaccgtgg cggacaatct atggcgtgga aagaagtcga
    23401 atctgcttgg agacgacgga gctccggtca ttgacatgga aagccggttc ttagagctgg
    23461 acctctgcga ttatcggaat tgcgagcggg cgacggaggg acaagatatc gtctatcacc
    23521 tggccgacgt ggttgccggc atcaactatg tgttcggcaa tcagttctcg ctgttcaata
    23581 caaatctgat catcgattcc aacatgctcc gggcggctat cgccaataag gtgtcgtcct
    23641 acgtgtatgt ggggacggca tgcagttatc cggccgaacg ccaaagcaaa ttgaacccgc
    23701 cgcctttcaa ggaagacgat gtgtatcccg catcaccgga gtcatcctat gggtggagca
    23761 agctgatggg agaaatggga tgtgatttgg ccagaaaaga gaagctgttg gatatcggaa
    23821 tccttcggtt tcacaacgtc tatggacccc actgcgaaat gtcgcccgag aaatcgcagg
    23881 tcattccatc cttgatccgc aaggcggtcc ggcatccgga tgaggaattc gtggtatggg
    23941 gatctgggaa acagcgccgc gcgtttgtgt tcgtgagcga tatcgtagac gccttggtgt
    24001 cagtggccac caaaggcctg aatcaggggg tgattcagat cggacctgat tacagcacct
    24061 cgattaagga gatcgcggaa ctggtgtgtg ccatttctgg aaagccgatc gatatccgat
    24121 tcgacaccag caagcccgaa ggtgatgtcg accgagcggc cgattggacc agagcgcgcg
    24181 acatcctcgg ctggtcgccc aagacgagca ttcaggaagg gctggagcgg acctatgcgt
    24241 ggtgtgatcg ccaggtgttt caaggcgtag ggcggtagga ggcggccggt ttctctcagg
    24301 tagatccagc gaactgaatg tgtggtgacc caggctggat gggtccgcgt atctcagttg
    24361 taacaacgaa gtgaggcaac atcgtgaaga aagcattgat caccggcatc agcgggcaag
    24421 atgggtcgta tctcgcagag ttattgttgg caaagggtta tgaggtccat gggattatcc
    24481 ggcgctccag ctctttcaat accggccgca tcgatgcgat ctatcaggat ccacacgttg
    24541 ccgaaccgag actacggctg gtctatggag acctcaacga tgctagttca ctcaataaaa
    24601 ttctgcgcac gatccagccg gatgagatct acaacctggg cgcgcagagc cacgtgcggg
    24661 taagtttcga tgtgcctgaa tatacggcgg aggtaacggc actggggacg gtccgtctgt
    24721 tggaagccat tcgcgaatcc ggtatccaac ccaagtttta tcaggcctcc tccagcgaga
    24781 tgttcggcaa ggtgcaggag attccccaac gggaaagcac accgttttat ccccgcagcc
    24841 cctatggcgc tgccaaggtc tacgcacact ggatcacggt caactatcgt gaggcgtaca
    24901 acctattcgc ctgtagcggc attctcttca atcatgaatc gccccggcga ggagaaacgt
    24961 ttgttacccg gaaaattacg cgtgccgcgg cccgaattaa gctgggggtg cagcaggagc
    25021 tgtatctcgg gaatctcgat gccaagcgcg actgggggtt tgccggcgat tatgtgcagg
    25081 cgatgtggat gatgttgcaa gcggatcaac cggatgacta cgtgatcgcg accggcgaaa
    25141 cgcatacggt gcgggaattt ctcgaccgca ccttcagcta tctcgatctg gattggaagc
    25201 aatacgtgaa gatcgatccg cgttattacc gtcccacgga ggtcgatttg ttgatcggcg
    25261 atgccacaaa ggcgaaaaag gtcttaggct gggagccgaa ggtatcattc gaagacctga
    25321 tcaccatgat ggtcgagggg gatctgcgcg ccgaacggag catcctcgat ggcacaagag
    25381 gagcacgctg atcatgtcgt tttggaaaga caagcgtgtt gtggtgaccg gcggggcggg
    25441 ctttctaggc acggtggtgg tcgaccaact tcggaagcta ggctgtcgaa acatcgtcgt
    25501 cccacggagc catgactatg atcttgtgga catgggcgac gtcaagcggt tgtatgtgga
    25561 tgcagctcct gatctggtgt tgcacctggc tgctcgcgtg ggcggcatcg gggccaatca
    25621 aacccatgcc ggtcagtttt tttacgacaa tcttatgatg ggaactcagc taatcgaggt
    25681 gggccgtcag caggggttaa aaaagttcgt ggcagtagga actatctgtt cctatccaaa
    25741 atatacgccg gttccctttc gggaagagga tctctggagt ggttatccgg aagagaccaa
    25801 cgctccctac ggcttggcga agaagatgat gctggtccaa gcgcaggcgt atcggcaaca
    25861 gtacggattc aacgccgtcg ttctgtttcc agtgaatctc tatggtcccg gggataattt
    25921 cgacatgcag acctcacatg tcattcctgc gttgatcaga aaatgcgtgg aggcaaagga
    25981 gcgtggtgac gacagagtcg tcttgtgggg cgacggctca ccgtctcggg agtttctgta
    26041 tgtgacagat gccgctcgtg ccattctgct cgctgctgag cattacaatg gaaatgagcc
    26101 ggtcaatctc gggaccgggc aggaaattac tattcaggcg ttggcgcatg tgattgccga
    26161 tgccgtgggg ttcacgggtg aaattgtctg ggacacaacg aagccgaacg gacagccgcg
    26221 ccgatgcctg gaagtgagcc gagccaagca actatttggg ttcgaagcaa gtactgcctt
    26281 tgttgacggt attcagcaga ccgttgcctg gttttacgcc aatcggggat ccatccggga
    26341 agtggtgttt tgactgttcc aacatcactt atctagttca tacaattcgg gtgcagtttt
    26401 ccatccgaga ggtacacgca agagagaaac gctgacgggg tcgtcgtgac gacatttttt
    26461 aatgctgtgc cttgttgtgt ccctggacga attaaacagt cataggggaa gacgccgggt
    26521 atcagccacg atggatgtgc cgaccacata gcaaatccgt ggatttgtgg atatcctgaa
    26581 ataatccgtg gtattccggt cgcaattttc tgtgccgccc agctagagtt ctgcgacatt
    26641 ccgtcaggaa agcctaattc catgaaaaat accggtttgg ttggcgccac tgtcttcact
    26701 tcttgcatga ctttgtctag agctgcgaag tgattctcga tgggaatcac gtagtcctgc
    26761 gtcattccag gccagtcctt cgcatagtaa tcgatcatgt agaaatttaa gctgatccag
    26821 tcgatgtatt gatcacctgc gtagaaattt gaaaaggcat gacagctttc tagcagctgg
    26881 cgcgcatagg ctttgtcgat ggtggagggc agttcattta cgtcatattc gatctgattc
    26941 acttggttca ctgaccatcc cattgtatca aaggtcagaa atttaaggcc ctcccgacgg
    27001 acgaagaaat catagtaata gcgttgcgcg gctttcagac gttcagttcc atcgcacact
    27061 tggggagttc caatgtcact gtagagtgac gcatacggaa gcgaagacgg attgaactca
    27121 gaaaacccgc gcttgttctc gatggcccag cgcagcgatt gatcaccagc tggtccaaag
    27181 ccgccaaaat agtctgctcc aattccgttc ggctctcgcc ctgcgataaa aaatacaggt
    27241 tttccgtatt gccttagttc tgcggcaaat cttcgaagtt ccgcgtcata gactccgttc
    27301 agcaacttat cgaccgtgaa tccctgaggg gcttcagact caccgggcgc agggtgtgtg
    27361 ttgtaggcct ggaccacgat cacttttcct tcctgccatg ccagattggc agcctgtacg
    27421 tccagatggg gttgacctgc cgcgtcgtat ccgttacccc acgagccacg atattttgac
    27481 cacagcgcag tctttcttcc gctggccgat tcaaacgatg caatatcatt ctggacccat
    27541 tcgtattggc ccatgtagag gccgccggct ggcggaggaa cgatcttcag tatcgaatag
    27601 tcacgtgcag cggtggtttg tgtcgtcggg ggtgtgacgg attgcgtttg tacctgtcgt
    27661 tttcgggaga accgagattg tgcttctgct gtagaggcgt cgaatgagat ccagattcct
    27721 gcaatgaagc tcagggcaag gactgctgca tagtgtattt tcacggtgag acctccgagt
    27781 atctggtcct tctagtgagc aagggtcgta ccatgggaag ggaaatgggg aagcccaaaa
    27841 atcaagggaa atggcaattt actcaggata tgcgctctgt ggcgaggcac attcttgaac
    27901 agaatgctgg tgggaccatt caggtcacat acacagaagg atacttgttc gtatccagca
    27961 ggtaggttct ctacggtttc tccatccccg gaatacattc agtcggcata agttcgccac
    28021 cgtgaaaatg tgagacttgt ttccatttct gcagcacggc tgcaccatcg aatgtcagca
    28081 gatattcttc acaccagatg ccgggcgcgg tgtaccgatt gccggcctgt tgttcgcgga
    28141 tttcatagac ccacaccgtc tctccctcgt cgctcttccg tactgcttta ggcgccccta
    28201 gttcctgcct cacctcagtc tgtgttgccc gccctttgac tgaatccaga tacgccgctt
    28261 ctctgttttg aaaatgactg caggcggtga tcccgatgag tatggccatc aggccgaagg
    28321 tccaagagcg tgtcatcatc tacctcccga ttcgagggtg atccggtttg gagatctatt
    28381 gtctcaggcg ggattgtaca ctgatcccat gaatccgtct gcctcatccc tcctcgcaga
    28441 tgggcggggg atgaacggcg aagaggcgca gaatgtcacg tactctagcc tggcgagggg
    28501 atagaattgt gcggtggtga taagggaacg agcggcgcga gagggcgcac ctcgagtgtg
    28561 aaatcaaatc atgggctgaa catggaaggg aagcgcggga
//
LOCUS       DAOQDS010000051        27579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_605_length_27579_cov_20.704803, whole genome shotgun sequence.
ACCESSION   DAOQDS010000051 DAOQDS010000000
VERSION     DAOQDS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 27579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27579
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_605_length_27579_cov_20.704803"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..1291
                     /locus_tag="PK589_13075"
     CDS             305..1291
                     /locus_tag="PK589_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNJ20585.1"
                     /translation="MLAEVSFIDTPSTLLLLSAGSHPFIVQASYLPMNIKDYLEQKRL
                     AVDRFLDEVSPPAATPPTTLHESMRYSLMAGGKRVRPILTIAAAEAVGTTPPGLMAVA
                     CSLEFIHTYSLIHDDLPSMDNDDFRRGKPTNHKVYGEAMAILAGDALLTMAFDLISRP
                     ELMKGCEPIRQVRILQELAYGSGNMGMVGGQVFDIQAENQDIDLPTLQNIHKHKTGML
                     IRAAVRMGAIAAGATDRQLDDMTGYAEDIGLAFQIADDVLNVTGTREELGKNPNTDAE
                     RGKKTYPTFYGVEGARKLADDCVTRAIGRLNTFGSSADPLREIARYITSRKN"
     gene            1400..2383
                     /locus_tag="PK589_13080"
     CDS             1400..2383
                     /locus_tag="PK589_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNJ20586.1"
                     /translation="MKALVTGATGFVGGAVARALVKAGVEVRVISRTGADLQNLAGLP
                     VEQVQGDLRDRDSLREALRGCRQLYHVAAHYALWAKDPSIFYDINVTGTRTLLETARE
                     VGIERTVYCSTIGAIGLPPGGGLGTEETPVSLEQMAGHYKRSKYLAEQEVLKLAREGL
                     PVVIVNPSAPVGEADVKPTPTGQIIVDFMKDRMPAYIETGMNLVDVDDVAQGHLLAMQ
                     KGRQGERYILGNKNLLLNEVFQILSRITGVKAPSVKLPRLAILPLAYANQWIANLTGV
                     PPRIPLEGVKMAKYKMHYDCSKAIRELGLPQTPVEVALEKAVRWFREHGYA"
     gene            2519..3328
                     /locus_tag="PK589_13085"
     CDS             2519..3328
                     /locus_tag="PK589_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNJ20587.1"
                     /translation="MEFFFLFLKTIWFRPYVFAFLAAFLFSAIMLIGWSRTWRFWLIS
                     WITAFVCEYSSTRNGIPFGWYHYNGSTIGQELYISNIPFMDSISFSFLLFASYCLALL
                     LLLPMAPGQRKPGFQLPRLTFALGDRTSWQVFALTVLFFAFIDMVIDPVALRGDRWFL
                     GKIYYYPDPGVHYGVPMANYAGWAVVGAISLLIYFPLDRRLATTLPAQTPSTTHRLLL
                     GAGLYYGVLIFNLAVTFWIGESLQGTTGVLMYVPVTAILLLRLLPPAPAAA"
     gene            3435..4052
                     /locus_tag="PK589_13090"
     CDS             3435..4052
                     /locus_tag="PK589_13090"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ20588.1"
                     /translation="MKKLCGLLLLGCLATLGILGWFGYQSYSTGFSAKAEPNELEVLL
                     ARQLRHLAIPYENRRLRNPLPVTQELMKEARAHFADHCASCHANNGSGDTVIGRNVYP
                     KSPDLRLPDTQTMSDGELFFIIQNGIRFTAMPGWGTGDPAKDSGSWQLVHFIRHLPSI
                     TEEELREMAALNPKTKQELQDEALIDQFLGGDDAAASSATGAHRH"
     gene            4076..4396
                     /locus_tag="PK589_13095"
     CDS             4076..4396
                     /locus_tag="PK589_13095"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNJ20589.1"
                     /translation="MTFLRLFIVALLLICAPAVVSAHGSGQHVLGVVSAIDATHIEIK
                     TPKGQIVSVRLTDKTQYKVRNLRRPKSPPQVGDRVVVEAEKGTDGLTATEVHYSDSQP
                     KAAQ"
     gene            4403..4876
                     /locus_tag="PK589_13100"
     CDS             4403..4876
                     /locus_tag="PK589_13100"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNJ20590.1"
                     /translation="MRVFQLSHNQQTGPGGDGLRPLVIRALIMGLAVFLAVTIVPGIE
                     SDSLGAGVAAVLVLTLLNSLIRPLLYLLALPLIVVSLGLFMVVINALLLQLTAALVKG
                     FTVAGFGASFWGALVISLVSSILNMLLVVEQSRVEPSHRPQRPPTIINPDGSEHS"
     gene            5076..6905
                     /locus_tag="PK589_13105"
     CDS             5076..6905
                     /locus_tag="PK589_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ20591.1"
                     /translation="MSPPVEPAKATAETHLQRTLTSVLNGLPADAALVAIFHQEQGPL
                     TTQVHRGFTPRDVQSIGRTLSSQKVLTAVPVGTDPEASRTMRLRLITPGAKSLLGVPL
                     RHRNRTYGFLVIGRKDNAVYAKKDKTMLEQAGDDITKALERDNLFDLNVLLSRPLVVQ
                     EPQPVIAAPDVYTPPTSHATPEIQGKIVTLLNELSQTLPFDRAWIGSYDPLAGNVEVL
                     GIAGEQKTDPKDQKKDLKAGQRLTLDASAAGWVVRHRKPRVDHDLASTQGRFLDHKHL
                     YKDRFQSSLVLPFFLRGQVGGTITLASKEADRYAVTDARLLEPINLKLVDLLQAAPPA
                     ATGAVKAEGAPDSEAGTTALTSAEPVIRKQERQAAIGEFSAFLATEIREPLGSIRAQL
                     EEVTAEGILDFDPQTRVENAMRDLIRIEAILNEILDFAKPLDLTRRLCRVPEMVENAL
                     TVVATELEATRIQVVKEYPQLLAPVRADEAKMQQVFLSIFKNAIEAMTPGGILTITMS
                     NQRAGKHLEVQILIKNNGAPIPPEHIDKVFEPFFTTKRSGTGLGLATVKKIVEEHQGS
                     IGISGAPGEGTTVTIRLPGVSRGPAHRYRGRGRRPPRRPTAAS"
     gene            7357..7722
                     /locus_tag="PK589_13110"
     CDS             7357..7722
                     /locus_tag="PK589_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20592.1"
                     /translation="MKLVIGSVATLAGVLFLCSMASANPALLPKHEGYPMKNDGSPVT
                     GQPTANDPGQKDARGESFLLKSAESTKHAEQKLTKTDNARITEGQGAGRLPNVQGPQI
                     KIEPPVTSATKITGERKID"
     gene            complement(7780..8427)
                     /locus_tag="PK589_13115"
     CDS             complement(7780..8427)
                     /locus_tag="PK589_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNJ20593.1"
                     /translation="MSAIESAGHRHLRFGLVRMFLGAAMILPREYFDRPTLEVARALV
                     GKYLVRDQGNRLLAGRIIEVEAYIGAEDKACHASKGRTARTDVLFGPPGHAYVYLCYG
                     MYEMLNVVTEQEGFPAAILLRAVECEGKLIDGPGRLTRAFDIDRRLNRCDLTLGQSLW
                     FEDRGETLTPGLLNAHPRIGVDYAGEWAHKPWRFRLTTETSRPRRLNAKRSLTRK"
     gene            complement(8734..10779)
                     /locus_tag="PK589_13120"
     CDS             complement(8734..10779)
                     /locus_tag="PK589_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNJ20594.1"
                     /translation="MTGALRYVVLWLALILWAAPLHAHDPDAVDLELPEVEVSADRPV
                     AASSQQFIPDKEYLMQPQGRPAQVLRLIPGFIAVEHSGGAGKADQYFLRGFDADHGTD
                     VAFFTDGMPINLRSHAHGQGYTDLNFIIPETIEGVDVYKGAYLPEYGDFATAGAVNFR
                     TRDMVKEGVVQSAGGQFNTQRHLLMFSPTKDTVRTLFAAEGYYTDGPFQNDNRYFRGN
                     LLGKATMNPLGRDELVITGTFQKSQWNGSGEIPLRAVHDGSLDRFGSIDPSEGGKTLR
                     STGRMNYHYDMPSGGRFFANAYAQYYRFDLFTNFTFFLNDPVNGDGFQQSDRRVVYGG
                     DVGYRHSGHLFDMDGAATVGVQARIDDIHARLAPQVKRAPLGSTADSTIFEASYAPFL
                     KLELQPTPWLRLAGGVRTEVFTFDVRNRCANCAEQPFGNTSTGLVLPKMNVILGPWFE
                     TEFFANYGEGYHSNDARSIVARAASPLAKARNYEVGVRSKPWGPDGVELIGTLWALDL
                     KQELVFVGDAGTTEIRGASRRRGLEVAARGQVWGPLYFNGSVTWTKAEFTNGDAIPLA
                     PEVTAYGALLLRWPEGLTSQLQATYLGVRPLVEDRSAKAPSWTTFDLSERYQLPVKLP
                     HGRLEAFLFVQNLFNTKWEQATFFFDSRLRNEAAGVADTHFVPGSPRFVMAGLAWYF"
     gene            complement(10787..11203)
                     /gene="nikR"
                     /locus_tag="PK589_13125"
     CDS             complement(10787..11203)
                     /gene="nikR"
                     /locus_tag="PK589_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNJ20595.1"
                     /translation="MDALARFGVSLDRHLLVDFDRLIERRKYTNRSEAIRDLIRDSLV
                     GQEWDENKDTVATITFVYDHHVPDLARKLTHIQHEFEGHIMAGMHVHLDHDHCLEVLV
                     ARGKGSAIRNVADALLSVKGVKHGKLTMTTTGKGLR"
     gene            complement(11263..12138)
                     /locus_tag="PK589_13130"
     CDS             complement(11263..12138)
                     /locus_tag="PK589_13130"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNJ20596.1"
                     /translation="MRVRRWNARSVWYLLAGLLVALAGWGFWWEPASLSTREYDLVLP
                     GWPAAAGDLRIAVLADLHTGSPHNDLEKLQEVVASTNGTHPDLILLAGDFVIQGVVGG
                     RVVEPEASARVLRSLQATLGVYAVLGNHDWWLDGPRVRKALEAEGIPVLEDRAIPLVF
                     NRMPFWLAGVGDFWEAAHDVRRALTGVDEASPVIVVTHNPDVFPEIPARVTLTIAGHT
                     HGGQVAVPGLGRPVVPSQFGERYAIGHIVEQGRHLFVSTGIGTSILPVRFRVPPEISI
                     VTLRSAAPLSQPPRD"
     gene            complement(12320..13393)
                     /locus_tag="PK589_13135"
     CDS             complement(12320..13393)
                     /locus_tag="PK589_13135"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ20597.1"
                     /translation="MQDVDLSTIKLLLVDDEAHCTKLMEAFLRQAGFSRITMTNDSRQ
                     ALELFQQVEPDLVALDMRMPHMDGFDVMRQLRQVIPADDYVPILIVTGELDAPTKHKA
                     LTEGANDFINKPVDATEVVLRIKNQLQTRRLHGQLRMHNEHLEAQVRLRTKVVEQTQL
                     DLLNRLVMASEYRYDPKGAHAWRVGRLAMLLSEMKGLPLEQTDMIKKTAPLHDVGKIG
                     LLDSIVMKEGSYTAIEWEAMKTHTKIGSKLLADSRSPLLMMAREIALTHHERWDGTGY
                     HRLKETQTPLPGRIVALVDAFDVMTHPRSYKGQLNLVEARAEIERHAGTQFDPELSAL
                     FLKLIDREGESLLADSTAVQLFA"
     gene            complement(13447..16200)
                     /locus_tag="PK589_13140"
     CDS             complement(13447..16200)
                     /locus_tag="PK589_13140"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ20598.1"
                     /translation="MQIEYSFLRTRLGRRVFVLFILCALLPVGVLSVVTIPQVTKYLR
                     TQAEEHMRQQSRAMGMAVYQRLLLLEEELLAITARQQVGASGRSEEWPSRPLSSRSGF
                     IGMASVSEADGTAVFGGGQRSRAFSSAERAILRQNKTLLTVEPAGVDRPRFFLSTMSE
                     QDGRVVVAEIKHDFLWDVGDSLAPPPDTSLCVFAQAGQTLLCTDAASVALSEQWLAQG
                     KASSGSFTWRDGDDEKLARAWGIPLRSRFSTDSWVVVLSKPMSVVLDQASDLRRQVRL
                     AIIAAVFASALLGVVQIRKFMGPLEQLGEGTRRIAARNFSTPVQVRSGDEFELLASSF
                     NAMAGRLNQQFTQLATIHEIDRSVLSVLDPSRIVDTVLTRVREVSPCDGIAMLLMDSS
                     DPARGWLYVRSGAKNKPTTMTGVSVGEEERRMLASHEGGTALIGVDGAAAFATLCRPG
                     VTALMVLPLFRSRDLLGAIAMSYRQAPEMDSEHKTQLRQLADQVAVALSNASDLAERK
                     RAEASLRESHTRLEQAMTDLQTAQQQMVQQERLRALGQMASGIAHDFNNTLSPIMGFS
                     ELLLIAPQMSADPAQLKEYLQTINMAAKDAAKVVSRLREFYRPRLDADLAGIVDLNRL
                     VEQSVKLSQPKWKDQALANGVAIEIKTELASVPPVAGHESELREVLTNLVFNAVDAMP
                     KSGSITLRTKADGDHVRLEVSDTGTGMTEEVRQRCLEPFFSTKGEKGSGLGLAMVYGI
                     IKRLRGTIEITSAVGVGTTFSIRLPIQSEQEAGTKQEGNSLAGVRLQILVVDDDPLVG
                     RVTGEYLRVAGHQVEVVTSAAEAVKRFNPERVHLVVTDHGMPNMTGRQLAEVIKQVSP
                     DTPVVLLTGGDEIEVTETASQAVDAELSKPLTMAALQRTLSTLTFPGSLPAESRKEIG
                     DAA"
     gene            complement(16216..16980)
                     /locus_tag="PK589_13145"
     CDS             complement(16216..16980)
                     /locus_tag="PK589_13145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNJ20599.1"
                     /translation="MPHIPFDRRQQGFTIIELAIVMVIVGALAGLAVPMYVGYLDKAR
                     LARCIAEIRYISRAVDSYNAAYDVYPNTLAEAGAGEIVDPWGNPYEYLNIAALSLPGN
                     GGGNGNAGGNGGGGGSGGNGSSGGGNGNTPGGSGGGGTGSSSGGTGGTNATTPPSNNG
                     NGGGGGNHGAWDWLLPVEAYAAGNANGNGNGNGSGNASKGKPRKDRFLHPINSDYDLY
                     SMGKDGESVEPLTAKKSHDDVIRANDGSFVGLAVEF"
     assembly_gap    17272..17371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17372..17982)
                     /locus_tag="PK589_13150"
     CDS             complement(<17372..17982)
                     /locus_tag="PK589_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNJ20600.1"
                     /translation="MSRPRFRRHFLWDTVQPRFVGLSLLYVVVVIAAVSGALFLPLML
                     QLDHLPLSSVEAQRVADQFELLHSRFWPAVGVVSLLLIVHGVFFTHRIAGPLYRFRRI
                     FQSVANGDLTVRTSIRKSDYLHAEAQCLGEMVSALREKIGRIEAHHADMAPQLERLKA
                     AAARGALREVEQEADRLRATVEQLAQAMEPFQTTSEPVEPRPTP"
     gene            complement(17979..18353)
                     /locus_tag="PK589_13155"
     CDS             complement(17979..18353)
                     /locus_tag="PK589_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20601.1"
                     /translation="MWSIRAVSCLLVLWWCAACAPEPDQAPSYLIGVWETTTDGYVDQ
                     HMFIDQTRIGFGTSALAADGYMITKVDEGRDGARTLFVVSYQDAEQAKYQLSFYYDPA
                     GGGRITFKNQDHLVWTKRAATT"
     gene            complement(18530..19963)
                     /locus_tag="PK589_13160"
     CDS             complement(18530..19963)
                     /locus_tag="PK589_13160"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNJ20602.1"
                     /translation="MSVRRSQPGLVALGIVGALLGCWPEGPASAADMLAPRFSKVSTN
                     GDLQAQVRATAAKVLPAVVSIASTVMVHDQGFSDEGLPFGMFKDVPPRRQYGQGSGVI
                     VSSDGYIITNNHVVADAVDVEVILADRRQFKGRVVATDPKTDVAVVKINASGLPTAAW
                     GDSSALAVGDFVLAIGNPLGLSRTVTFGIVSAVGRADVGVADVEDFIQTDAPINPGNS
                     GGALVNTNGELVGINTAIASPTGGSVGVGFAIPSNMARTAMQSLIKTGRVVRGFLGAS
                     TQDVTPLLAKIFHLPDVKGSIITDLQAKGSAERAGLKRGDVVVRFDGRDVMDSGHLRN
                     LMAAAAIGSKHRVELLRDARLMQAELTVQEAPRERQRKTQSAETAGPTAHPLSGVIVD
                     EITPALARQMDLPSNTGLVVTDIEDGSLAEVSGLQPGDLLLELNRQPIPNFSTFQRLA
                     EPLRSTDLALLLINRQGSLLYIPIQTE"
     gene            complement(20031..22928)
                     /locus_tag="PK589_13165"
     CDS             complement(20031..22928)
                     /locus_tag="PK589_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNJ20603.1"
                     /translation="MARDPVCGMTVEPATAAGYAEYEGETYHFCSPSCRDRFRAAPAH
                     YVKKAAPAGAAVQPSSRRSLPMMQAMPAQEATTGGERDPVCGMMVEPATAAGSHTHEQ
                     KTYYFCCQGCLEKFRADPGRYLAPVAAPASTSLRQFGGKALPMLGAASSPGPSAAAVI
                     DPVCGMTVDPATAAGSFAYQDTTYFFCCQGCLTKFRADPARYLSPGSVKDAMPATPVP
                     PGTKYVCPMCPEVLEEKPVPCPKCGMALEPDSVQPLPTKTEYVCPMHPEVVQAEPGAC
                     PKCGMALEPRTVTVAEELNPELVDMARRFWVALGPAAIVFLLAMSHMLPGHPIQHLLS
                     DTQSAWVQFVLSTPVVLWAGWPFFQRGWASIVHRSPNMFTLIAIGTGTAYLYSVIATL
                     FPFSIPQSFHLESGAVPVYFEAAAVITVLVLLGQVLELRARSRTTGAIRALLGLAPKT
                     ARRLFDDGREEDVPLEQVQVGQRLRVRPGERVPVDGVILEGATSIDESMITGESIPVE
                     KFAGERVTGGTINGSGGVVMRADRVGADTLLSHIVQMVAEAQRSRAPIQRTADVVAGY
                     FVPVVVSVALVTGILWAWLGPEPRLAHALLNAVAVLIIACPCALGLATPMSIMVGTGR
                     GASAGVLFKKAESLETIEKVTTLVFDKTGTLTEGKPKLRVVSAMPPWSETDLLRLAAS
                     VERGSEHPVANAIVGGAEARGLNLEPAAGFTSKAGKGVMATVGTKRVAVGTIDWLRDL
                     KVGDEAALAALDANAELMRQTGQTVMLVAVDGQAIGLLGVADPIKSSTPEALRLLRQE
                     GIRLVMVTGDHAVTAQAVAKELGLDEVRAGVKPEDKSRIVQELQRQGQVVAMAGDGIN
                     DAPALAQADVGIAMGTGTDVAMENAGVTLVKGDLRGIVRAYRLSKATMRNIRQNLFFA
                     FVYNSIGVPIAAGLFYPVFGLLLSPMIASAAMTGSSLSVISNALRLRQTDL"
     gene            complement(23037..23681)
                     /locus_tag="PK589_13170"
     CDS             complement(23037..23681)
                     /locus_tag="PK589_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNJ20604.1"
                     /translation="MVMRRVWHGGLFVGVMVMALVGLAGTAQAAKPELKGNFQVLSEE
                     KSTHKPGKVQLVEFADFYCPHCHRFDGEGLMILEKEFGNKVEAVMVGYPVIPGKLPTA
                     FDMYEQAKSMGKGNEMKRALFRTIHKDKVGIIDKAIREVLIREVGLNPAAFEEGLASA
                     KPAKAFEDGRKWGDRIKIQQTPTVLLDGNIKVEQIDPENLKLIIQSILDGDSKK"
     gene            23778..24329
                     /locus_tag="PK589_13175"
     CDS             23778..24329
                     /locus_tag="PK589_13175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNJ20605.1"
                     /translation="MRGKLLQGLGILCMTAILAACATSADQREAEDGASQTPQFSQIK
                     AAPESFKGQTVVLGGQVLSARRMKDGTRLEVLQLPLNDAQQPSLDLTKSQGRFIAIRR
                     EFLDPATVPQGTFVTITGQMTGSVTQPLDETDYAYPVLEITTFRTWIPSQDLNLPRYR
                     PYPYYSPFWHPYWGPYGRFPYYW"
     gene            complement(24485..24964)
                     /locus_tag="PK589_13180"
     CDS             complement(24485..24964)
                     /locus_tag="PK589_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20606.1"
                     /translation="MLSRSAVVLFGALSLLSSATGAWALQAGGVEVGDLETGSVVGQP
                     FKELEVDAQLFAVEVGGLKAWYPPTTIIDFKVRPGRPVLLKVTNNTSSDRGFQMTDPV
                     NAASPFVLKAEVVLKPGETKYIGVPTSDLFNATQGNTLTYRDQLNPKQPGGQLIMIK"
     gene            complement(24983..25327)
                     /locus_tag="PK589_13185"
     CDS             complement(24983..25327)
                     /locus_tag="PK589_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20607.1"
                     /translation="MRCVCIVLIQLLVVAGGTAGWGADDSLEHLLRAQAFNKEFEGFD
                     FYYVNIEADHPQPDGSHEVLAIASGKFSDSTKRMKVLFLIVDGLIVGGQVLEGTGLPP
                     CLAPEHRSSSSL"
     gene            complement(25569..25871)
                     /gene="smbP"
                     /locus_tag="PK589_13190"
     CDS             complement(25569..25871)
                     /gene="smbP"
                     /locus_tag="PK589_13190"
                     /inference="COORDINATES: protein motif:HMM:NF028095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HNJ20608.1"
                     /translation="MTGLRAGVVVASLCALVAVAPANMALADGGGHVKETIKHAKEGV
                     EHEKEAIKHLEEAVKGSNDPHAKEALEHAKEAVKHAEESLAHAEQASGKKGKGKGN"
     gene            complement(26081..26605)
                     /locus_tag="PK589_13195"
     CDS             complement(26081..26605)
                     /locus_tag="PK589_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20609.1"
                     /translation="MPVSDAVASPQRTTVAGKPYDQTGLNVAVVRLLALALDISGGWS
                     PAPPAAGLMIPFGYRDWPHLTSLVQSSGGAQELRFYVCPKGLLTADDRSFPVGTVFVV
                     ESEPCPVGIGAQESRPAVFVMEKCAGMTRDEVGTAQSELWIYASWDSGGQSARADPGR
                     CGICRLPWLTPASA"
     gene            complement(26637..>27579)
                     /locus_tag="PK589_13200"
     CDS             complement(26637..>27579)
                     /locus_tag="PK589_13200"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ20610.1"
                     /translation="DDARVALAANQLGLLYHDQGRLSEAEALYLRALGTWEAQLGPEH
                     EDVAAALNNLAEIAHAKGEWAAAEPLYERVLKIERQTLGPAHPDVAISLNNLAELYRK
                     QGRETAAESTYRLALTLMEQAHGPDHVELGPVLNNLAALYKGRGMYAWAEPLYERALK
                     VRRLRFGNDHPAVVMSLNNLACLYQAEGLYASAQRHFDEALPLAERLSGPQSALAGTI
                     IGNMAFLADQQGLMAQAQLWYQRALVIQQQQLGVHHPTVGLLVERYAVVLNSIGQSVE
                     AGLYAARAASIRARSQPGAVKQGAVSYLGATVGEASR"
BASE COUNT         5661 a         8882 c         7555 g         5381 t
ORIGIN      
        1 gaagaatatt catggggcgg aagtataacg aaggggtcga agaaaaaaca aggagggagg
       61 ggtaggaggg cggccctagt ggtcccccca tgatcgcgca acgcgcggcc tgggaaggcc
      121 ctcgttggac gcgctcactc tgggaggacc accagaccgc cctcctaaca agatagacgt
      181 aagaagaagg cgcacgatcg gaatgtgccc gtcccatgcg cagtgaagcg ccaccaggcg
      241 agtccctcgg taagagacgt aggcatgatc gtatgcgcaa gccacgcgtt ccacgagcag
      301 gaggatgctg gcagaagtca gcttcattga caccccttca acccttttgc tactctccgc
      361 cggttcccat ccattcatcg tccaggcttc gtatctaccc atgaacatta aagactatct
      421 cgaacagaag cggctcgcag tggaccgttt tctcgatgag gtcagtcctc ccgctgccac
      481 accgcccacg acgctccatg agagcatgcg gtacagcttg atggccggcg gcaagcgcgt
      541 tcgaccgatc ctcaccatcg ccgccgccga ggcggtcgga accacaccgc cggggctgat
      601 ggccgtggcc tgttcgctgg aattcatcca tacctattcc ctgattcacg acgatttacc
      661 ctcgatggac aacgacgact ttcgccgggg caagccgacc aatcacaagg tctacggcga
      721 agcgatggcg attctggccg gcgacgcact gctgaccatg gccttcgatc tcatcagccg
      781 gcccgaactc atgaaaggct gtgagcccat ccgtcaggtg cgcatccttc aggagctggc
      841 ttacggatcc ggcaacatgg gcatggtggg cggccaggtg ttcgacatcc aggccgagaa
      901 tcaggacatc gatctcccca cgctacagaa tatccataaa cataaaaccg gcatgttgat
      961 ccgggccgcc gtgcgcatgg gcgcgattgc ggccggcgcg acggatcggc agctcgatga
     1021 catgaccggc tatgccgaag acatcggctt ggcctttcag atcgccgacg atgtgctgaa
     1081 tgtcaccggt acccgcgagg aactcggcaa gaatcccaat actgatgccg agcgcgggaa
     1141 gaagacctat ccgacgtttt atggcgtcga aggcgcgcgc aagctggcgg acgattgtgt
     1201 gacccgcgca atcggccggc tcaacacatt cggctcctca gccgatccgc tccgcgagat
     1261 cgcgcgctat atcacctcgc gaaagaactg acctgaggcg tgaaacgtaa gacgtgagac
     1321 gcaatccctt cgatcgcgcg cccctctcac gtcacccacg gttcaccgtt cacgctgtcc
     1381 gcttcacgga aattcaggca tgaaagctct cgtaaccgga gcgaccgggt ttgtcggcgg
     1441 ggccgtcgca cgcgcgctgg tgaaagccgg ggtagaagtc cgggtcatct cccgaaccgg
     1501 tgctgatctg cagaacctgg ccgggctccc ggtcgaacag gtgcagggcg acctccgcga
     1561 tcgtgactca cttcgtgagg cgttacgcgg ctgccggcag ctctatcacg tggcggcgca
     1621 ttacgcgcta tgggccaaag atccctccat cttctacgat atcaatgtca ccggcaccag
     1681 gacgctgctt gagacggccc gtgaggtcgg catcgaacgt accgtctatt gcagcaccat
     1741 cggtgcgatc ggcttgccgc cgggcggcgg cttaggcact gaggagacgc cggtgtccct
     1801 cgaacagatg gcggggcatt acaaacgatc caaatatctc gccgaacagg aagtcctgaa
     1861 actggcacgc gaaggcttgc cggtcgtcat cgtgaacccc agcgcgccgg tcggggaagc
     1921 agatgtaaaa ccgacaccca ccggccagat catcgtagac ttcatgaagg accggatgcc
     1981 tgcctatatc gaaacgggca tgaacctggt cgatgtggat gacgtggcgc agggccatct
     2041 gctcgccatg cagaaaggcc ggcagggcga acgctacatt ctcggcaata agaatttgtt
     2101 actcaatgaa gtatttcaga ttctcagccg gattacgggc gtcaaggcgc cctcagtgaa
     2161 actccctcgc ctggccattc tgccgttggc ctatgcgaat caatggatcg cgaacctgac
     2221 gggcgtgcca ccccgcatcc cgctcgaagg cgtgaagatg gccaaataca aaatgcatta
     2281 cgattgttcg aaggccattc gcgaactggg tctgcctcaa acaccggtcg aggtcgcgct
     2341 agagaaagcg gtgcggtggt ttcgcgagca tggctatgct taacggatgc tgaaaaaggc
     2401 tttgagctgc gttctcggct cgacaagatc ctcaacgtac cctcaagggt acgcctccgg
     2461 tcttgccagc gcctgcggcc ttgctaaaag acctttttga gcatcctcca cttgcccgat
     2521 ggaattcttc tttctgtttc tcaaaacgat ctggtttcgc ccctacgtct ttgcctttct
     2581 ggcggcgttt ttgttttcag cgatcatgct gatcggctgg tcgcggacct ggcgattctg
     2641 gctcatcagc tggatcaccg cattcgtgtg cgagtattcc tcaacgcgta acggcattcc
     2701 cttcggctgg tatcactaca acggctcgac gatcggacaa gagctgtaca tttccaacat
     2761 tccattcatg gattcgatct cgttctcgtt cctcctgttc gccagctact gcctcgctct
     2821 tctcttgctt ctgcctatgg caccgggcca gcgcaaaccc ggtttccagc tcccccggtt
     2881 gacgtttgca ctcggcgacc gaacgtcatg gcaagtcttt gccctgaccg tcttattctt
     2941 tgcctttatt gatatggtga tcgatccggt ggcgcttcgc ggcgaccgct ggtttctggg
     3001 caagatctat tattacccgg accccggcgt ccattacggc gtccccatgg ctaactacgc
     3061 cggatgggcc gtggtcggcg ccatctccct cctcatctac tttccgctcg accgccgcct
     3121 cgccaccaca cttccggccc agacgccttc caccacacat cgcctcttgc tgggcgcagg
     3181 cctctactac ggcgtgttga tcttcaacct ggccgtcacc ttctggatcg gcgagtcact
     3241 gcagggcacg accggcgtct tgatgtatgt gccggtgacc gccatccttt tgctccgatt
     3301 actcccccct gcacctgcgg ccgcataaat cacgcgctcc tgcctgtggc aaaaggggcg
     3361 caattggcat tcattggaat gagactgtat agtggtaaac aggcgcgatg cctgcgcggg
     3421 aaggcttgtc tctgatgaaa aaactctgcg gactgttgtt gctcggctgc ctcgcgaccc
     3481 tcggcattct cgggtggttc ggctaccaat cctactcgac cggatttagc gccaaggccg
     3541 aaccgaacga attggaagtc ctgcttgcca gacaactgcg acacctggcc attccctatg
     3601 agaatcgacg actccgcaac cccctgcccg tcacgcaaga gttgatgaag gaagctcgcg
     3661 cccatttcgc cgaccattgc gcttcctgcc atgccaacaa cggcagcggg gacacggtga
     3721 tcggcaggaa tgtctacccc aagtctccgg atctccgcct gccggatacg cagacgatgt
     3781 cggacggtga gctgttcttt atcattcaga acggcatccg gttcaccgcc atgccgggct
     3841 ggggcaccgg cgatccggcg aaggacagcg gaagctggca actggtgcat ttcatccgcc
     3901 acctgcccag catcacggag gaagagctgc gggaaatggc cgcgttgaac cctaaaacga
     3961 agcaagaatt gcaagacgaa gccctgatcg atcagtttct gggcggagac gatgccgcgg
     4021 catccagtgc caccggcgca caccgccatt aacgcctcaa cgaaaggatt ttcccgtgac
     4081 attccttcgc ctcttcattg ttgcactcct cctcatctgc gccccggctg tcgtatccgc
     4141 ccatggcagt ggccagcatg tgctcggggt cgtctcagcc atcgatgcca cccatatcga
     4201 aatcaagact cccaaaggcc agatcgtctc ggttcgtctg acggacaaga ctcaatacaa
     4261 agtgcgaaat ctgcgccgcc cgaaaagtcc gcctcaagtg ggagaccggg tcgtcgtcga
     4321 agcggagaaa gggactgacg gtctgacggc gacggaagtc cattattccg attcccaacc
     4381 gaaggcggcc caataactca ccatgcgcgt gtttcaactc tcgcacaacc agcagaccgg
     4441 acccggcggc gacggtcttc ggcccttggt gatcagagct ttgatcatgg gcctcgccgt
     4501 cttcctggcc gtgaccattg tgcctggcat cgagtccgac agtctcggtg ccggcgtcgc
     4561 cgccgtgctg gtactgacgc tgctgaacag cctgattcgc ccgctcctgt atctgctggc
     4621 gcttccgctc atcgtcgtgt cgctcgggct gttcatggtt gtcatcaacg ccttgttact
     4681 gcaattgacg gctgcgttgg tcaaagggtt caccgtcgcc gggttcggcg catcgttctg
     4741 gggcgccctc gtcatcagtc tggtcagcag tattctcaac atgctgctgg tggtggaaca
     4801 gagccgggtc gagccgagcc atcgccctca acgcccgccg accatcatca atcccgacgg
     4861 gtcagagcac tcgtgaacgc tgcgactttc cttgagttac tctgatatga ctgttacaat
     4921 gcgccccgac gctcgatctt caccgcagag agcgcacaac ctataacgag catcgtgttt
     4981 cgatcgtgta gcgggtctga ttcacggtca gcgcaaagac gccgatcgac aatccccgcc
     5041 ccctgcatca tcggaaacat cctgcacaga ggtcgatgag tcccccagtc gaaccagcga
     5101 aagccacagc cgaaacccat cttcagcgca ccctgacctc cgtcctcaac ggattgccgg
     5161 ccgatgccgc gctggtcgcg attttccacc aggaacaggg cccactcacg acgcaggtgc
     5221 accggggctt tacgccgcgg gacgttcaat cgatcggccg cacgttgtcg tcccagaagg
     5281 tgttgacggc cgtgccggtc gggactgacc ccgaagcgtc gcgcaccatg cgccttcggc
     5341 tgattactcc tggagccaag tcgctgctgg gcgtgccgct ccgccaccgc aaccgcacct
     5401 atgggtttct ggtgatcgga cgcaaagaca atgcggtcta tgcaaaaaaa gacaagacca
     5461 tgctcgagca agccggggac gacattacga aggccctcga gcgcgacaac cttttcgatc
     5521 tgaatgtgct cctcagccga ccactggtgg tgcaggaacc gcaaccggtc attgccgcac
     5581 cggatgtcta cactccgccg acttcccacg cgacgccgga gatccagggc aagatcgtca
     5641 ccctgctcaa tgagttgagc cagaccctgc cgttcgatcg cgcctggatc ggttcctatg
     5701 atccgctcgc cggcaacgtc gaagtactgg gcatcgccgg ggaacaaaag accgatccca
     5761 aagaccagaa aaaggatctc aaagctggcc agcgactgac gctcgatgcc tccgctgccg
     5821 gctgggtggt gcgtcaccgg aagccccggg tcgaccatga cctcgcttcc acgcaaggcc
     5881 gctttctcga tcacaagcat ttgtataaag accgcttcca atcttcattg gtattgccct
     5941 tcttcttgcg cggccaagtc ggtggaacca tcacgctggc ctcgaaggaa gcagaccgct
     6001 atgcggtgac cgatgctcgc ttgctcgagc cgatcaacct caaattggtg gatttgctcc
     6061 aggctgcgcc cccggcggca accggcgccg tcaaagccga aggcgcgccg gattccgagg
     6121 ccgggaccac agcattgacc tccgccgagc cggtcatcag aaagcaggag cggcaggctg
     6181 ctatcgggga attcagcgcg tttctggcca ctgaaatccg tgagccattg ggctcgatcc
     6241 gcgcgcagtt ggaagaagtc acggcggaag gcattctcga tttcgacccg cagacccgtg
     6301 tcgagaacgc gatgcgcgac ctgatccgca tcgaagcgat cttgaatgaa atcctcgatt
     6361 tcgccaaacc gctggacctc acgcgccgcc tctgtcgcgt ccccgaaatg gtggaaaacg
     6421 cgttaaccgt ggtggccacc gaactggaag ccacccgcat tcaagtcgtc aaagaatatc
     6481 cccagctcct cgcgccggtg cgcgccgatg aagcgaagat gcagcaggtc ttcctcagca
     6541 ttttcaaaaa tgcgatcgag gccatgaccc cgggcggcat cctgacgatc accatgagta
     6601 accagcgggc cggcaaacat ctggaagtcc agatcctgat caagaataac ggggcaccga
     6661 tcccgcccga acatatcgac aaggttttcg agccgttctt tacgacgaag cgttccggca
     6721 ccggcctcgg tctcgccacg gtcaagaaga tcgtcgagga gcatcagggc agcatcggca
     6781 tttccggcgc accaggcgaa ggcacaaccg tgacgattcg cctgcccggc gtcagccgtg
     6841 ggcctgcgca ccgctatcgt ggccgcggac gacgcccgcc gcgtcgcccc accgccgcat
     6901 cgtagctgct gacctccaag gattcgatcc gaaggttaga cctgaagcga atcttttcag
     6961 tgcgcctacg cctgacatca aacaacacaa tattctcgtt ccagacgcgt gcacgccggc
     7021 ctggcatgcc atcaccccag ccgcctatgg acaggcgggg tgagcctcct cctgcgcacg
     7081 tccaacgagg gccttctgag gccgcgctca tcttcaacaa tcaccgcaca cacctgcggg
     7141 tggtcatgga ggccgtccaa ctgcgcgcat gggccgagca cattccgatt gtgcgcggtc
     7201 tgcgagcaac tggatggcct cagaaccacc cgttcccctc ccccctttat tcttgacacg
     7261 gcttcttcag gaagctaaga ttccggcact cttaaggctt ttaacgtcta tatactcgac
     7321 caccatactt tcacattctt tcaaaggagt aggggtatga aactcgtgat cggcagcgtc
     7381 gcgacccttg caggtgtatt gtttctgtgt tcgatggcct ccgcaaaccc ggcgctgttg
     7441 ccgaagcacg aaggttatcc gatgaagaac gacggcagcc ccgtcacggg acagccgaca
     7501 gccaatgatc ccggccaaaa ggatgctcgc ggcgaatcct tcttgctgaa gtccgccgag
     7561 tccaccaagc atgccgagca gaagctgacc aagacggaca atgcgcgtat caccgaaggc
     7621 cagggcgcag gccgtttgcc gaacgtgcag ggtccgcaga tcaagattga gccacctgtg
     7681 acctcggcga ccaagatcac cggcgagcga aagatcgact aattctcgat tactagaatc
     7741 cgtgctatga aggggaagcc gtacatcggc ttcccctttt tattttctgg tgagggaccg
     7801 tttggcattg agacggcggg gcctggatgt ctccgtcgtc aggcgaaacc gccagggttt
     7861 atgagcccac tccccggcgt aatccacccc gatacgcgga tgggcattga gcaagcctgg
     7921 cgtaagcgtc tcgccacgat cctcaaacca aagtgactgg ccaagtgtca ggtcgcagcg
     7981 attcaatcgg cgatcgatat cgaaggcccg ggttaaccgg cccggtccat cgattaattt
     8041 tccttcgcat tccacggcac ggaggagaat tgccgccgga aatccctcct gctccgtgac
     8101 cacattcagc atttcataca tgccgtagca gaggtagaca taggcatggc ccggtggacc
     8161 gaacaacaca tccgtacgtg ccgtccgccc cttcgaagca tgacaggcct tatcctccgc
     8221 gccgatgtag gcctccactt cgatgatccg tccggcaagc agcctgtttc cctgatcgcg
     8281 cacgagatat ttccccacca gcgcacgggc cacctcgagt gtgggacgat cgaaatactc
     8341 acgaggaaga atcatggccg ctcctaaaaa catccggaca agcccgaatc gtaaatgccg
     8401 atgccccgca ctttcaatcg ctgacatcac cggaacttct ttgttcagtt cccctcgcga
     8461 ccgctcaaac cgtcatccag caaagcctca gctgacgcag cagcggagtc ccgatccttg
     8521 agaccgctca aaaatggctt cccaacaagg ccgcaggtga gaaaataccg caggcgtacc
     8581 cgcattctgc tggtgatgtg ccctcagaca ggccgctcaa aatgtccgtt cagctaggcc
     8641 gcagccgagg gtagcgccgg aggcgtagcc cgtggctacg ttgaggacgg taccgaggcg
     8701 agaacaacgc tggcgggcat tttcagcagc ctgtcagaag taccaggcga gaccagccat
     8761 cacaaaccgt ggactccccg gcacaaagtg cgtgtccgca acgccggccg cttcgttacg
     8821 tagacgggag tcaaaaaaga acgtggcctg ctcccatttc gtgttgaaga gattctgcac
     8881 aaagagaaat gcctccaacc ggccgtgcgg cagtttcacc gggagttgat atcgctccga
     8941 cagatcgaac gtagtccagg acggcgcctt cgcgctgcgg tcttcaacca gcggccgaac
     9001 ccccagatac gtcgcttgca gctgggatgt cagcccttcc ggccaccgca acagcaacgc
     9061 gccataggcg gtcacttccg gcgccaacgg aatcgcgtct ccgtttgtga attccgcctt
     9121 ggtccaggtc acgctaccgt taaagtagag cggcccccag acctgacccc gtgccgcgac
     9181 ttccaggccg cgccgtcgcg aagccccacg gatctccgtc gtccctgcat ctcccacaaa
     9241 gacgagttcc tgtttcaggt ccagcgccca caaggttccg atcaattcaa ctccgtcagg
     9301 cccccacggc ttcgaacgaa caccgacttc gtaatttctc gctttcgcca atggtgaggc
     9361 agcccgggcc acgattgagc gggcgtcgtt actgtgatac ccctcgccgt agttcgcaaa
     9421 gaattccgtc tcgaaccagg gccccagaat cacgttcatc ttcggcagca cgagccccgt
     9481 actcgtattg ccaaacggct gttccgcaca gtttgcacaa cggtttcgca cgtcgaacgt
     9541 gaagacttcg gtgcggacac ctccggccag tcgcaaccaa ggcgtaggct gcagctccaa
     9601 ttttaagaag ggcgcgtagg aggcttcgaa aatcgtgctg tccgcggtgg atcccagcgg
     9661 cgcccgtttc acttgcggtg caaggcgcgc atggatgtcg tctatccgtg cctgtacacc
     9721 cacggtcgcg gctccgtcca tgtcgaacag atgtccactg tgacgatacc ccacatcacc
     9781 gccgtagacc acgcgccggt ctgattgctg aaagccgtcg ccgttgaccg ggtcgttgag
     9841 aaaaaacgtg aaattcgtaa acagatcgaa ccgataatac tgcgcatagg cgttggcaaa
     9901 gaaccgcccg cctgatggca tgtcgtaatg gtaattcatg cgacccgtgc tacggagcgt
     9961 cttcccgcct tcgctcggat cgatcgatcc aaagcgatcc aacgagccat catgcaccgc
    10021 gcgaagcgga atctcgccgg acccgttcca ctgcgacttt tgaaacgtgc cggttatgac
    10081 caattcgtcc cgacccagcg ggttcatcgt ggccttgccc aacaggttgc cgcggaaata
    10141 ccggttgtca ttctggaacg gcccgtcggt gtaataccct tccgctgcga acagcgtccg
    10201 tactgtatct ttggtcggcg aaaacatcag gagatggcgc tgggtgttga actgtccacc
    10261 ggccgactgg acgacgcctt ccttcaccat gtcacgcgtg cggaaattca ccgcgccggc
    10321 tgtagcgaaa tctccatact cgggcagata ggcccctttg tagacatcga cgccttcaat
    10381 ggtttccgga atgatgaagt tcagatcggt gtagccctgt ccatgggcat gacttcgcaa
    10441 attgatcggc atgccgtcag tgaagaatgc gacatcggtg ccatggtccg catcaaaccc
    10501 gcgcaggaag tactgatcgg cctttccggc cccacccgaa tgttcgaccg caatgaatcc
    10561 ggggatgagc cgcaagacct gggccggacg gccctgcggc tgcatgagat attccttatc
    10621 gggaatgaac tgttgagagg atgctgccac cggccgatca gcagacactt cgacttccgg
    10681 cagctcgagg tccacggcat ccggatcatg ggcgtgaagc ggcgcagccc acaggatcag
    10741 agcgagccac agcacgacgt aacgcagcgc accagtcacc ggcggctcac cgtagccctt
    10801 tgccggtggt cgtcatggtc aacttgccat gcttcacgcc cttgacgctg agcaacgcgt
    10861 cggccacgtt gcggatggcc gaccctttgc cacgcgccac caacacctcg aggcaatgat
    10921 catggtccaa atgcacatgc atcccggcca tgatgtgccc ctcaaactcg tgctgaatgt
    10981 gggtcagctt ccgggcgagg tccggcacat gatggtcgta cacaaacgtg atcgtcgcga
    11041 ccgtatcctt gttctcatcc cactcctgac cgaccagact gtctcgaatg agatcgcgaa
    11101 tcgcctcaga gcgattcgtg tacttccgcc gttcgatcag gcgatcgaaa tcgacgagca
    11161 gatgacgatc gagtgacacg ccgaatcgcg cgagtgcgtc catgccgcct ccttcaacgg
    11221 tgctacaaat attttttttg taacactgcg atgaaagacg cgtcaatcac gaggaggctg
    11281 actgagaggg gctgcggacc gaagggtgac gatcgatatt tccggcggca cgcgaaagcg
    11341 aaccggcagg atactggtgc cgatgcccgt gctcacaaac aaatgccgcc cctgttcgac
    11401 gatatgccca atggcatacc gctctccgaa ttgagacggc acgacaggcc gtcctaatcc
    11461 cggcactgcc acctgcccgc catgtgtatg gccggcaatc gtcaaggtca cacgggccgg
    11521 aatctcggga aagacatccg ggttgtgcgt cacaacgatc accggacttg cctcgtccac
    11581 gccggtaagc gcgcggcgaa catcatgcgc cgcttcccaa aaatcaccga caccggctaa
    11641 ccagaacggc atccggttga acacgagcgg gatggcacgg tcctccagca ccggaatgcc
    11701 ttcggcttca agcgctttcc gcacacgcgg accatcaagc caccaatcgt ggttgcctaa
    11761 caccgcgtag actcccagcg tcgcctgcag cgagcgaagg acccgggcgc tcgcttccgg
    11821 ttcaacaaca cgtccgccca ccacaccctg gatcacaaaa tcccccgcca acaggatcag
    11881 gtccggatgc gtgccattgg tggatgccac cacctcctgc aacttttcga gatcgttatg
    11941 aggtgagccg gtatgcagat ccgccaaaac cgctatccgc aggtcacccg cagcggcagg
    12001 ccatccgggt aagacgagat catattctcg ggtagacagc gatgcaggct cccaccagaa
    12061 gccccagcca gccaaggcaa ccaacagccc ggcgaggaga taccaaacgc tgcgagcgtt
    12121 ccatcgcctg acgcgcatgg ccattaagac cgcgacctca taaactggta ccaatcgggt
    12181 gatgtctctg ccgaccgaac gcagtcagcg tcgaacatcg ccatctcgtc gcagtctctt
    12241 ctcgcgaaca ggcgatcatg ggatgacgat gctcgtgtct gatcagacag cctgatctgc
    12301 cggtggggaa acgaggggac tatgcaaaca actgcacggc cgtcgagtcg gccaggagac
    12361 tctcgccttc acgatcgatg agcttgagaa agagggcgct caactccgga tcaaactgcg
    12421 tccctgcatg tcgctcgatc tcggccctgg cctccacgag attcagctgg cctttgtacg
    12481 aacgtggatg ggtcatgaca tcaaatgcat ccacgagcgc cacgatccga ccgggcaagg
    12541 gagtttgcgt ctccttcagg cgatgatatc cggtgccgtc ccatcgttca tgatgcgtca
    12601 acgcaatttc tcgtgccatc atcaagagcg gagaccggct atcggccaag agtttcgacc
    12661 cgatcttggt gtgcgtcttc attgcttccc actcaatcgc agtataggaa ccttctttca
    12721 tgacgatgga atccagcaga ccgatcttcc ccacatcatg aagcggagcg gttttcttga
    12781 tcatgtcggt ctgttcaagc ggcaatccct tcatctccga cagcagcatg gccagacggc
    12841 ccacgcgcca ggcatgggcc cccttgggat catagcggta ttcggaggcc atgaccaagc
    12901 gattgagcag atccagctgc gtctgttcca cgactttggt gcgcagccgg acctgggctt
    12961 caaggtgctc gttgtgcatc cgcaactgcc cgtgaagacg tctggtctgc agctggttct
    13021 tgatccgtaa caccacttcg gtggcgtcga cgggtttatt gatgaaatcg ttggcccctt
    13081 cggtgagcgc cttgtgtttc gtcggcgcgt ccaactcacc cgtcacgatc aaaatcggga
    13141 catagtcgtc ggcaggaatg acctgtctta actgtcgcat cacgtcaaac ccatccatgt
    13201 gcggcatgcg catatcgagg gcgacgagat ccggctccac ctgttggaac agctccagcg
    13261 cctgacgaga gtcattggtc atcgtgatgc ggctaaatcc ggcctgccgg agaaaagcct
    13321 ccatcagttt ggtgcaatgg gcttcatcat cgaccaacag cagtttgatg gtgctcagat
    13381 caacgtcttg catcgcgctc acaccgctcc tctgtccgca gactcctacg gtcgagatta
    13441 ccaccgtcag gccgcatcgc caatttcttt tctgctctcc gcagggagcg aaccggggaa
    13501 cgtgagagtc gacaaggtcc gctggagcgc cgccatggtc aacggcttgc tcaactcggc
    13561 atcgacggct tgggaagcgg tctccgtgac ctcaatctcg tcgccgcccg tcagcagcac
    13621 caccggcgtg tccggcgaca cctgtttgat gacttcggcc aactgtcgcc cggtcatgtt
    13681 cggcataccg tgatcggtca cgaccaggtg cacccgctcg gggttgaacc gcttcaccgc
    13741 ttcggccgca ctggtaacga cctcgacctg atggccggcc acccgcagat attcgccggt
    13801 cacacgaccc accagcggat catcatcgac gacaagtatc tgcagccgca ccccggccag
    13861 gctgttgcct tcctgtttgg tgcccgcctc ctgctcggat tgaatcggca aacggatgct
    13921 gaaagtcgtc cccaccccga cggcactggt gatctcgatc gtcccgcgca gccgtttgat
    13981 gatcccatac accattgcca acccgaggcc ggaccccttt tcgcccttgg tggaaaaaaa
    14041 cggctccaga caacgctgac ggacctcctc ggtcatgccc gtgccggtat cgctgacttc
    14101 aagacgcaca tggtcgccat ccgccttcgt ccgcagcgtg atcgacccgc tcttcggcat
    14161 ggcatccacg gcgttgaaga cgagattcgt cagcacttcc cgcaattccg attcgtgtcc
    14221 ggccaccgga ggcaccgaag ccaattccgt tttgatctcg atggccacgc cgttcgccaa
    14281 ggcctgatcc ttccatttcg gctggctgag cttcacactc tgttcgacga gtcgattgag
    14341 atccacgatg ccggccaggt ccgcatcgag gcggggccga taaaattcgc gcagacggct
    14401 gacgactttc gccgcatcct tggcggccat attgatggtc tggagatatt ccttgagttg
    14461 cgcgggatcg gccgacatct gcggcgcaat cagcaacagt tcgctgaacc ccataatggg
    14521 cgacagcgtg ttattgaaat catgcgcgat gccgctcgcc atttggccta aggcgcgaag
    14581 ccgttcctgc tgcaccattt gctgctgtgc ggtctgcagg tcggtcatgg cctgctcgag
    14641 ccgcgtatgg ctttcacgca gcgaggcctc tgcacgcttc cgctccgcca gatcagaagc
    14701 attactcagc gccaccgcca cctgatcggc caactgacgc aattgcgtct tgtgctcgga
    14761 atccatctcc ggtgcctggc ggtaactcat cgcgattgca cccagcagat cgcgtgagcg
    14821 gaacaagggc agcaccatca gagccgtcac gcccggtcta cagagggtgg caaacgcagc
    14881 ggctccatcc accccgatca gcgcggtgcc gccttcgtgg gaggccagca tccggcgctc
    14941 ttcttcgccc acagacacgc cggtcatcgt cgtcggcttg ttcttcgccc ccgatctgac
    15001 atagagccag cctcgcgccg gatcggacga atccatcagc agcatggcga tcccgtcgca
    15061 aggcgacacc tcacgcaccc gggtcaatac cgtatccaca atgcgactgg gatcgagcac
    15121 cgacagcacc gagcgatcaa tctcatggat cgtggccaac tgggtgaact gttgattcaa
    15181 ccgaccggcc atcgcgttga acgacgaggc tagcaattcg aactcatcgc cgctccgcac
    15241 ctgcaccggc gtcgaaaagt ttcgcgcagc gattcgcctg gtgccctcgc ccagttgttc
    15301 cagcgggccc ataaacttcc gaatctgaac cacaccgagg agggctgagg caaacaccgc
    15361 cgcgatgata gccaggcgga cctgacggcg gaggtcgctc gcctgatcca acaccacact
    15421 catgggttta ctcagcacca ccacccagga atccgtcgag aatcttgacc tgagcgggat
    15481 gccccaggcc cgcgccagct tctcgtcatc gccatctcgc catgtgaaac ttccggagct
    15541 ggctttgccc tgcgctagcc actgctccga cagcgctacg gacgcagcat cggtgcaaag
    15601 gagcgtttgc ccagcctggg cgaaaacaca cagtgaagtg tcgggcggtg gcgcaagact
    15661 atcgcccaca tcccataaaa agtcgtgctt gatttccgcg accaccactc ggccatcttg
    15721 ctccgacatg gtggaaagaa aaaaacgagg tcggtcaaca ccagcgggtt cgaccgtgag
    15781 gagcgtcttg ttctgacgaa gtatcgcacg ctccgcagag ctgaaagcgc gggaccgctg
    15841 cccaccacca aacaccgccg ttccgtcggc ttccgagaca gaggccatgc cgatgaatcc
    15901 cgaacggctc gatagcggcc gcgagggcca ctcttcggac cgcccggaag caccgacctg
    15961 ctgtcgtgcc gtgatcgcaa gcaattcctc ctccaatagc agcaaccgct ggtacacggc
    16021 catgcccatg gccctgcttt gctgccgcat gtgttcttcg gcctgcgtcc tgaggtattt
    16081 cgtcacttga gggatcgtga cgacgctcaa cacgccaacg gggagtagtg cacagaggat
    16141 gaacaggaca aagacgcgcc ggcccagacg agtgcggagg aaagaatatt cgatctgcac
    16201 aatagcttcg tctcgttaaa actcaacggc cagcccaaca aagctgccgt cattcgcgcg
    16261 gatcacgtcg tcgtgacttt tcttggctgt gagcggttcc acactctcac catcttttcc
    16321 catgctatac aggtcgtagt cggagttgat tgggtgcagg aagcgatcct ttctcggttt
    16381 gcccttgctc gcattccccg atccgttgcc gttgccattc ccgtttgcat ttccggctgc
    16441 gtaggcctcg acggggagga gccagtccca ggcaccgtgg tttccaccac ccccgccatt
    16501 tccattgtta ctgggtgggg tcgttgcgtt tgtgcccccg gttcctccag aactactccc
    16561 agtacctcct ccaccgcttc cgcccggagt atttccattg cctccaccac tgctcccatt
    16621 tcctcctgac ccgccacctc caccgtttcc gccagcattc ccgttaccac caccattccc
    16681 tggcaaactc aaggcggcaa tgttcagata ctcgtaggga ttcccccagg ggtcgacgat
    16741 ctcgccggct cctgcttcgg ccaatgtgtt gggatagaca tcataggcgg cgttgtagga
    16801 atcaaccgcg cgggagatgt aacgaatttc ggcaatgcag cgcgcgagcc tggctttatc
    16861 caagtacccc acgtacatcg gcacggccaa cccggccaga gcgccgacaa tcaccatcac
    16921 aatggccagt tcgataatgg tgaatccctg ctggcgacga tcgaagggaa tgtgaggcat
    16981 aacgatccta gaatgcagag caaaaactcg cttattgcgc aacacgctac agctcactgc
    17041 cgtgccgcct cgctttggct gtcacccgca ggatgcgcaa aaagtccgtc cagcaaggcc
    17101 gcagcaaacg aagaggcgaa tcgtactcgc cccgtacggt gagcctctga gtgccgcgag
    17161 aacgccgctg gcggactttt tccgcatcct gctagaatcc tgcagcggtg ggcaaaggcg
    17221 tgggacgcgg ctcaaccggt tcagaggtgg tctggaacgg ctccatcgcc tnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcgtggga cgcggctcaa ccggttcaga
    17401 ggtggtctgg aacggctcca tcgcctgcgc cagttgttcg acagtggcgc gcaatcgatc
    17461 agcctcctgc tccacttcgc gtaaggcacc acgagcggcc gcggccttca gccgctccaa
    17521 ctgcggggcc atatcggcat ggtgcgcctc gatccggccg atcttctccc gcaacgcact
    17581 gaccatttct cccaaacact gggcttcggc atgcagatag tcgctcttgc gaatcgaggt
    17641 gcgcacggtc agatccccgt tcgccacgga ctggaaaatc cggcggaagc gatagagcgg
    17701 gccggcgatg cggtgcgtga aaaatactcc gtggacgatc aacaataacg acaccacccc
    17761 gacggccggc cagaaccggc tgtgcaacag ctcaaactga tcggccactc gctgcgcctc
    17821 aaccgaggac aggggcaggt gatccagttg caacatgagc ggaagaaata acgcgccgga
    17881 gacggccgcg atgaccacca ccacgtagag gagactcagg cccacaaacc tcggctggac
    17941 cgtgtcccac aaaaaatgcc gtctgaaacg tggcctgctc atgtggtcgc cgcccttttg
    18001 gtccaaacca aatgatcctg gtttttaaac gtgatccgtc cgcctccggc gggatcgtaa
    18061 taaaacgaca attggtactt cgcctgctcg gcatcctgat aggacacgac gaagagcgtt
    18121 ctggccccat cgcgaccttc gtcgaccttc gtaatcatgt agccgtccgc cgcgagcgcg
    18181 ctggtcccga atccgatccg ggtttggtca atgaacatat gttgatcgac atacccatcg
    18241 gtggtggttt cccacacgcc gatcagatac gacggagcct gatccggttc aggagcacaa
    18301 gcggcacacc accacagcac cagcagacaa ctcaccgctc tgatcgacca cacaagtcac
    18361 ctcttcccat ggggtgtcgg cgaaatcgac tcgacacgga atcagtatgt cgcagttgcc
    18421 gggaaataca atttctttaa aagttcctac acgcagggcg gagggtcaga aaaacagcgt
    18481 cgggccgccg gtaggtgcca gcaacgagag cggaagggtc agaacacgac tattcggttt
    18541 ggatcgggat gtagagcaaa ctgccctgcc ggttgatgag cagcagagcg agatccgttg
    18601 aacgaagcgg ctcggcgagc cgttgaaagg tgctgaagtt gggaatgggc tgtcgattga
    18661 gttccagcag cagatcacct ggttgcaggc ccgatacttc cgccaggctg ccgtcttcga
    18721 tatccgtcac caccaggccg gtgttggaag ggagatccat ctggcgcgcc agcgcgggag
    18781 tgatttcatc cacgatcaca cctgaaagag gatgcgctgt gggaccagcc gtctcggcag
    18841 actgcgtctt ccgctgccgt tcgcgcggcg cctcctgcac ggtcaattcg gcttgcatca
    18901 agcgggcatc ccgcaggagt tcgacccggt gtttgctgcc gatggcggcg gccgccatca
    18961 gattgcgcag atggccgcta tccatgacat cacgcccgtc aaaccgcacc accacgtcgc
    19021 cgcgcttcaa cccggcacgt tcggcagagc ccttggcctg cagatcggta atgatcgacc
    19081 ccttcacgtc cggcaggtga aaaatcttcg ccagcaaggg cgtcacatcc tgggtcgatg
    19141 cgcccagaaa tcctcgcacc acacggcctg ttttgatgag actctgcatg gcggtcctgg
    19201 ccatgttgct gggaatcgca aagcctacgc cgacgctccc gcccgtagga ctggcgatgg
    19261 cggtgttgat ccctaccaac tctccattgg tattgaccaa ggcgccaccc gaattgccgg
    19321 gattgatcgg cgcgtcggtc tgaatgaaat cttccacatc ggcgacaccc acatcggccc
    19381 gtccgactgc gctcacgatg ccgaaggtca ccgtgcggct caggccgagc gggtttccga
    19441 tcgccagaac aaaatcaccg acagcgagcg cactggaatc gccccaggct gcagtgggaa
    19501 gcccgctcgc attgattttt acgacggcca catccgtctt cggatcggtc gcgacgacgc
    19561 gccctttgaa ctgccggcgg tcggccagga tgacttccac gtccaccgca tcggccacca
    19621 cgtgattgtt cgtgatgata tacccgtccg acgagacgat gaccccggat ccctgcccgt
    19681 attggcggcg aggcggcaca tccttaaaca tgccgaacgg caggccttca tcgctgaacc
    19741 cctgatcgtg caccatgacg gtggacgcaa tgctgaccac ggcaggaaga accttggcgg
    19801 cggtagcccg cacctgcgcc tgcaaatcgc cgttggtgct caccttggag aaacggggcg
    19861 caagcatatc agccgctgag gcaggtcctt ccggccaaca gcccagcagc gcgcctacga
    19921 ttcccagagc caccaggccc ggctgtgatc gacgtaccga catggtgtga cacatcctca
    19981 agacggcagg gcccacccta tcacgcacag ggttcacgca cacgcagaac tcataaatcg
    20041 gtctgccgca agcgtagcgc gttcgaaatg acggagagcg aactccctgt catcgcagca
    20101 ctggcaatca tcgggctcag gagaagacca aacacgggat agaagagccc ggccgcgatg
    20161 ggcacaccaa tactattgta cacaaaggcg aagaagagat tttgtcggat gttcctcatc
    20221 gtggccttgc tcaagcgata ggcccggacg attccccgca gatcaccctt cacgagcgtg
    20281 actccggcat tttccatggc gacatcggtg ccggttccca tcgcgatccc gacatcggcc
    20341 tgggccaagg caggcgcatc gttgatgcca tctcccgcca tcgccacgac ctgaccttgc
    20401 cgctgcagct cctgcacgat ccggctcttg tcttccggtt tgacgccggc ccgcacctca
    20461 tccagaccca attccttcgc cactgcctgt gctgtaacgg catggtctcc ggtgaccatg
    20521 accaggcgaa tgccttcctg cctcaacaag cgcagcgcct caggggtgga gctcttgatc
    20581 gggtcggcca cgcccagcaa tccgatcgcc tggccatcca ccgccaccag catcacagtc
    20641 tgtccggtct gccgcatcaa ttcggcattg gcatccaacg cggcaagggc tgcttcgtcg
    20701 cccaccttca ggtcccgcaa ccagtcgata gtccctacgg caactcgctt cgtcccgacg
    20761 gtcgccatga cgcctttccc cgccttcgag gtgaacccgg ccgccggctc taaattcaac
    20821 cctcgagctt cggccccgcc gacaatcgcg ttggcgaccg gatgctcact accccgctca
    20881 accgacgcgg ccaaccgcaa cagatcggtt tccgaccagg gtggcatcgc actgactacc
    20941 cgcaactttg gtttgccttc cgtcaacgtc ccggtcttgt cgaacaccag cgtggtgacc
    21001 ttttcgatcg tctccaacga ctcagccttt ttgaacaaga ctccggccga cgcaccccgc
    21061 cccgtcccca ccatgataga catgggcgtg gccagcccca gcgcgcaggg gcaggcaatg
    21121 attaacactg ccacggcatt caacagggca tgggctagac gaggctccgg tcccagccac
    21181 gcccatagaa tgccggtgac gagcgccacg ctgacaacga ctggaacaaa atacccggcc
    21241 accacatcgg ctgtgcgctg aataggcgca cgactgcgct gagcctcggc caccatttgc
    21301 acgatgtggg acaacagcgt atccgcgccc acccgatcgg ctcgcatcac cacgccaccc
    21361 gatccgttga tggtgccacc ggtgacccgc tcaccggcaa acttttcgac ggggatcgat
    21421 tcgccagtaa tcatggactc atcaatcgag gtcgcccctt cgagaatcac tccatccacc
    21481 ggtacccgtt caccaggacg aacccgcaac cgttggccga cctgcacctg ttccagcggg
    21541 acatcttctt cacggccatc atcgaacaac cggcgggcgg tcttcggggc caagccaagc
    21601 agcgccctga tcgctccggt cgtccgactc ctggcccgca gctccaacac ctggcccaag
    21661 agcaccagga cggtgatcac cgcggccgct tcgaaataga ccggcaccgc accgctctcg
    21721 agatggaagg attgcggaat cgaaaacgga aatagcgtcg cgatcacact gtagagatag
    21781 gctgtcccgg tgccgatcgc gatcagagtg aacatgttcg gactgcggtg cacaatcgac
    21841 gcccaacccc gctgaaagaa cggccagccg gcccacagca ccaccggcgt gctgagcacg
    21901 aactgcaccc aggccgactg ggtatccgac agaagatgtt ggatcggatg gccgggaagc
    21961 atgtgcgaca tggccaacag aaacacgatc gccgccggtc ccaatgccac ccagaaccgt
    22021 ctggccatat cgaccagctc gggattcagt tcctccgcga cagtgaccgt tcgtggttcc
    22081 agcgccatgc cgcacttcgg gcaggcaccc ggctccgcct gcaccacctc cggatgcatc
    22141 gggcagacat attccgtctt ggtcggcagc ggttggacgg aatccggttc cagagccatg
    22201 ccgcatttcg ggcaagggac cggcttctcc tccaatactt ccggacacat ggggcagaca
    22261 tacttggttc ccggagggac cggggttgca ggcatcgcat ctttgacgga gcccggcgat
    22321 aggtagcgcg ccggatcggc gcggaacttg gtgagacaac cttggcagca aaagaaatag
    22381 gtcgtgtctt ggtaggcgaa ggaaccagcc gccgtcgccg gatccaccgt catgccgcag
    22441 accgggtcga tgacagccgc cgcggagggc ccgggggacg aggcggcccc caacatgggt
    22501 aaggcctttc ctccgaactg acgcagcgat gtcgatgcag gagcggcgac gggagccagg
    22561 taccgacccg gatcggcccg gaacttttcg agacagccct ggcaacagaa ataataagtc
    22621 ttctgttcat gggtatgaga gccagccgcc gtggccggct caaccatcat cccgcagacc
    22681 gggtcccgtt cgccgcctgt ggtcgcttct tgcgcaggca tcgcctgcat catcggtagc
    22741 gagcggcgac tggatggttg gacggcggca ccggcggggg cggccttctt cacgtagtgc
    22801 gccggcgcgg cgcgaaaccg gtcccggcat gaggggctgc aaaaatgata ggtctcgccc
    22861 tcatattccg cgtaccctgc cgccgtggcc ggctctaccg tcatcccgca tactggatca
    22921 cgtgccattc gcgcttgtac ctcgtcgttc gtcgctcgta tctcgtcgct cgtcggttcc
    22981 gtcttacgtt tcacctttca cgtttcacga aatacgcttc acgagatacg gcctcgttac
    23041 tttttgctgt ctccgtccag gatgctttgg atgatcagct tcaaattctc cgggtcgatt
    23101 tgctccacct tgatgttgcc gtcgagcaac acggtcggag tctgctggat cttgatgcga
    23161 tcgccccatt tccgcccgtc ttcgaacgcc ttcgccggtt tcgcactggc cagcccttct
    23221 tcgaaagccg ccggattcag tccgacctcg cgaatcaaga cttcacgaat cgccttgtcg
    23281 atgatgccta ccttgtcttt gtgaatggtc cggaacagcg cccgcttcat ttcattgccc
    23341 ttgcccatac tcttggcctg ctcatacata tcgaaggccg tcggcaactt gccggggatg
    23401 acgggatagc ccaccatgac cgcttcgact ttgtttccga attccttctc cagaatcatc
    23461 agcccttcgc catcgaaccg gtggcaatgc ggacaataga agtcggcgaa ctccaccaac
    23521 tggaccttac ccggcttgtg ggtcgatttt tcttcgctca agacctggaa attacccttg
    23581 agttcgggct tcgccgcttg cgctgtgccc gccaatccca caagcgccat caccatcacg
    23641 cccacgaaca gaccaccgtg ccacacacga cgcatcacca tacgctgcac tcctttttca
    23701 aacatgaatc gttgccacag gctcctcggc attataaaca atgctggcac tcttctgcgt
    23761 ctgttataat gccaacgatg cgtgggaaac ttctacaggg tctcggtatt ttgtgcatga
    23821 ccgcgatcct ggccgcttgc gcgacctccg ccgatcagcg tgaagccgaa gacggcgcat
    23881 cccagacgcc gcaattttcg caaatcaaag ctgccccgga atcgtttaaa ggacagaccg
    23941 tcgtgctggg cggccaagtc ctctcggccc ggcggatgaa agacggaacc cgcctcgaag
    24001 tgcttcagtt gccgctcaat gacgcgcaac agccatctct ggatctgacc aaatcgcagg
    24061 gccgattcat tgcgattcgc cgggaattcc tggatcctgc gaccgtgccg cagggcacct
    24121 ttgtcaccat tacgggccag atgacgggct ccgtcactca gccgctggat gagaccgact
    24181 acgcttaccc ggtgctggag atcaccacgt ttcgcacgtg gattccttct caggatttga
    24241 accttcctcg gtaccgcccc tacccctatt acagtccgtt ctggcatccc tattggggac
    24301 cgtacggacg ttttccttac tattggtagc accgtcaccc gcactctcgc ctggaatcat
    24361 cgcccgtttt ctgcgccttg tcggaccgca tgctcgagct gatcgtggct tacggccctg
    24421 aacgacaacg ccccgtccat ccggagatga acggggcgct gcgagaaccg aattgattcg
    24481 gtgtttactt gatcatgatc aactggccgc cgggctgctt cgggttcaat tgatcccgat
    24541 aggtcagggt gttgccctgg gtggcgttga acaaatcgct ggtcggcaca ccgatgtact
    24601 tcgtctcacc gggcttgagg accacttcag ccttcagcac gaacggagag gctgcgttga
    24661 ccgggtccgt catctggaag ccacggtcag aagacgtgtt gttcgtcacc ttcagcaaga
    24721 ccggacggcc gggacggact ttgaagtcga tgatggtggt cggtgggtac cacgccttca
    24781 ggccgcccac ttcgaccgca aacaactgcg catccacttc caactccttg aacggttggc
    24841 cgaccacaga accagtctca aggtcgccca cctcgacgcc gcccgcctgc aacgcccagg
    24901 ctcccgtggc gctcgacaac agactcaacg caccaaacaa taccactgca ctcctgctca
    24961 acatacacaa cctcctgtcg ggttaaagag atgaagacga acgatgctcc ggagccaggc
    25021 acgggggaag accagtgcct tccagcacct gaccgccaac aatgagacca tccacgatga
    25081 gaaacaacac cttcatgcgc ttcgtgctgt ctgaaaattt gccgctggcg atggccagca
    25141 cttcatgcga tccgtcgggc tgaggatgat ctgcttcgat gttgacataa tagaaatcaa
    25201 atccctcgaa ctccttattg aacgcctggg cccgcaacag atgctctagc gagtcgtcgg
    25261 ccccccatcc cgcggttcca ccggccacca ccagaagttg gatcaggacg atacagacac
    25321 atcgcatgag gctacctccc tagggtggga gttatagcct cgaggcaccc ggtcgtctac
    25381 agacgcacgc tcatggatgc ttacgggtgc tacagtatcg tttccggacc accaacggca
    25441 agaggttata caggattacg gcgcctgagg gaaccagggg aagaccgagg gtagaactgg
    25501 aggctctggc aggaagccac gggggaagcg gaatccttga tgcttcccac cgtagcctca
    25561 tcgttcggtt agttgccctt tcctttgcct tttttgcccg aggcctgttc ggcatgggcc
    25621 agggattctt ccgcatgctt cacagcttct ttggcatgct ctaacgcctc cttcgcatgc
    25681 ggatcgttgc tgcccttcac cgcttcttcc aagtgcttga tcgcttcctt ctcatgctcc
    25741 acgccttcct tggcatgctt gatcgtttct ttcacatgcc cgccgccgtc ggccagcgcc
    25801 atgtttgccg gcgcgaccgc cacgagcgca catagagacg caaccactac tcctgcacgt
    25861 aatcctgtca tacgcattcc tcctgtgtca tagtgaaccg ttggctgatt ggccgcattc
    25921 tacagcattt tgtagacggt gttgagcgag atggtggtat tgagagtggt caccgggaaa
    25981 gaaaaaggtg ggagagggaa gcgtatgggc gtacgcctcc ctcatcggag ctcactgcgt
    26041 ggtcagcgca gtccaaccga cgagctccgt ccaccgatcg tcaggcagag gccggggtga
    26101 gccacggcaa acggcagatg ccacaccgcc cgggatccgc tctcgccgac tggccgccgg
    26161 aatcccagct cgcatagatc cacaattcag actgtgccgt cccgacctcg tctctcgtca
    26221 tccccgcgca tttttccatg acaaacaccg ctggacgaga ttcctgcgcg ccgattccta
    26281 ccggacaggg ttcggattcc accacgaaca ccgtccccac gggaaatgat cgatcatccg
    26341 ccgtgaggag ccccttcggg cacacgtaaa accggagctc ttgcgctccg ccagacgact
    26401 gcacaagcga cgtaagatgg ggccagtctc gatacccgaa cgggatcatc aatccggctg
    26461 cgggcggagc cggcgaccac cctccgctga tgtccaacgc cagcgccaac aagcgcacca
    26521 ccgctacatt caatcctgtc tggtcgtacg gcttcccggc gaccgtcgtt cgttgaggag
    26581 atgccaccgc gtcactgacc ggcatggacc accttccttt ctgatcgggg cacaggttat
    26641 cgactggctt ctccgaccgt cgcgccaagg taagagaccg cgccctgctt caccgctccg
    26701 ggctgagacc gtgcgcgaat cgatgcggcg cgcgccgcat atagtccggc ctccaccgat
    26761 tggccgatgc tgttgagtac cactgcatat cgttccacca ggagtccaac cgtggggtgg
    26821 tgcaccccga gctgctgttg ctgaatcacc agcgcgcgct gataccacag ttgcgcctga
    26881 gccatcagtc cctgttgatc ggccaggaac gccatgttgc ctataatcgt gccggccaga
    26941 gcgctctgag ggccggagag ccgctcggcg agcggcaagg cttcgtcgaa atggcgttgc
    27001 gccgaggcat acagcccctc ggcttggtac aggcaggcca ggttgttcaa actcatcacc
    27061 acagccggat ggtcgttgcc gaagcgaagc cgacggacct tcaaggcccg ttcgtacaac
    27121 ggttcggccc aggcatacat cccgcggccc ttgtataacg cagccaggtt attgagcacc
    27181 ggccccagct caacatgatc ggggccatgc gcctgctcca tcagcgtgag cgccaatcga
    27241 tacgtcgatt cggcagccgt ctctcgcccc tgcttgcggt agagctcggc cagattgttc
    27301 aagctgatcg cgacatcggg atgcgccggg cccagcgtct ggcgttcgat cttcagcact
    27361 cgctcataca atggttccgc cgcagcccac tcgcctttgg catgggcgat ttccgccaga
    27421 ttgttcaatg cggcggcgac gtcttcgtgc tccggcccca gttgagcctc ccacgtgccg
    27481 agcgcgcgca gatagagtgc ttcggcttct gagagccggc cctggtcgtg atacaacaat
    27541 cccagctggt tcgcggccag cgccactcgc gcgtcatcg
//
LOCUS       DAOQDS010000052        26511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_648_length_26511_cov_22.284246, whole genome shotgun sequence.
ACCESSION   DAOQDS010000052 DAOQDS010000000
VERSION     DAOQDS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 26511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26511
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_648_length_26511_cov_22.284246"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PK589_13205"
     CDS             <1..837
                     /locus_tag="PK589_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ20611.1"
                     /translation="RHPDVQTYAEESTSWPSVSRPTYAGGLGFGMKWDMGWMHDTLEY
                     MGLDPVYRKHHHRNLTFRMLYAFQENFLLPLSHDEVVHGKGSLLGKMPGDDWQKFANL
                     RTLFGYMYAQAAKKLIFMGGEIGQWREWAHDDSIDWNLLNYPPHQGLQRWVADLNHLY
                     RAEPALHEFDFDPRGFEWIDCQDVDAGTISLLRHGRSSRESIAVVCNFTPVPRLQYRV
                     GVPQGGYWKELLNSDASLYGGSGLGNLGGLSADAVPAHGRSHSLALTLPPLAVLFLKS
                     AS"
     gene            933..1424
                     /locus_tag="PK589_13210"
     CDS             933..1424
                     /locus_tag="PK589_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20612.1"
                     /translation="MMAIRSCRTLIWQTPLMLVIALSLAHCSHDIHEKRADTIKGHVE
                     SFYDHLKHDRVAAAVRENEAIEQLSTQLGEIVTRRVNQPGTNQIDREWTDLRTANETA
                     AQNWLALGQYLSIKKQYAQSRATYQRVIDTYNGATERTYREQAARAIRDLDILSPPPA
                     APR"
     gene            1450..2001
                     /locus_tag="PK589_13215"
     CDS             1450..2001
                     /locus_tag="PK589_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20613.1"
                     /translation="MRFIASRLMILVMGLCLLLTGCVHRIHVNPASNSPATITIPAAV
                     QLEVPFLAIEGADHMPGIAMLDWPAKDLRQAIVRYFSERQTFRAIGTDPAELKMVVKA
                     WLTLRAPDRYLYRVHLEADLSHPGHGVFKTYAAEGEALGSSVRWITASDQEPIDEATS
                     QALQQLATQLEQDRDLIMNNFSR"
     gene            2442..3617
                     /locus_tag="PK589_13220"
     CDS             2442..3617
                     /locus_tag="PK589_13220"
                     /inference="COORDINATES: protein motif:HMM:NF021508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HNJ20614.1"
                     /translation="MILCFDGEWDCPPDPGVWSFSANHSPKQSRTGVQPDSHSATNVY
                     QLYQSILPRSGDGRIQQKWYEPGTTMPWFHRFRDGSFGCGLDRTILQSYAYLAATYDP
                     GDELYLYGFSRGAYTARSLVGLLTLTGLPAPVLLNADHVRLVREAASPHSPSGGVAAH
                     LRECLTRMVTDPDNHVIDEAYRVYRNLQNASQIATGRRKGSSDINVTLLGLWDTVGPL
                     GIPTKALRWLNEHRYNFRDTELSPIVRHACHALAIDEHRADHNATLWTSPPKAAQVIE
                     QRWFAGAHGDLGGTYPDRDLADVPLAWIRQHSMAKGLALESGRIDEKLNMLGPIHDSF
                     SECFVGFRRWIHPRFYRPVMQTGTGTEVLDESVHTRLLHSPAYRPKNEGLNNLLTFR"
     gene            3842..4204
                     /locus_tag="PK589_13225"
     CDS             3842..4204
                     /locus_tag="PK589_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20615.1"
                     /translation="MKRSSQTKLLVHCILSDVAMGCAEEGKVFDVGQRGTLRTTFHNL
                     IDGDHLSVKIQLRDERRPVSVHLAKVSWIQGERFGVELLVMDVDERLRLNRFLEAKLP
                     LELEFQDSHTELTIRAAD"
     gene            4628..4807
                     /locus_tag="PK589_13230"
     CDS             4628..4807
                     /locus_tag="PK589_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20616.1"
                     /translation="MSIDVELALVLGLFMIGAVVLAIDNLVYALSKRFSRWMRNRKSV
                     PRTPSTFTSTQKKQF"
     gene            complement(5562..6992)
                     /locus_tag="PK589_13235"
     CDS             complement(5562..6992)
                     /locus_tag="PK589_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNJ20617.1"
                     /translation="MRSLALVLFSTLLAACAVGPDFNRPEATTPDAFRMAEPGGDAAS
                     IANTPWWELLKDQELQKLIRTALEENKDLKRAAAAIEEFQARLFIAKTDYAPQMNVTA
                     NAPLFGRKSNFLFPGFPNPFNYYLQGNLSWEIDIWGRIRRSNEAARGDLLSREENRRA
                     IVLQLVSGVAEAYFDLLQFDMQLDIARRTLKSWEESVRIAQARLRQGMISKLDADQFE
                     AERANAAAKAAEFERQKVQKENQLSVLLGRNPGRIARGYSLTEQVMPPDVPPGLPSEL
                     LQRRPDILQAEQDLAAATARIGMAKADRFPKLSITGILGVASPHLSRLVANETAFGVA
                     GPGLAGPLLNAQILGFQQRAAEAQARQAVAQYEQSVLVAFKEVEDSLVAVRTVREQRA
                     AQLQQVEALRSALSLANLRYKGGLANYLDVLIAQRNLFEAELALMGTHRLHLVSIVQL
                     YKALGGGWTPEGQLPVSPIASPPEKS"
     gene            complement(6985..10167)
                     /locus_tag="PK589_13240"
     CDS             complement(6985..10167)
                     /locus_tag="PK589_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNJ20618.1"
                     /translation="MISHFFIDRPIFASVLSIVIMVLGLVALQALPIAQFPEITPPVV
                     QIEADYPGANAEILADSVARPIEVQLPGIDNLLYYDSTSTNDGHMSIKLTFEIGTDVD
                     IAQVQTQNRVKLAEPQLPPEVVRQGISINKVSPDLLAVVALSSTDPTHDTVYLSNYAI
                     LRVLDNLKRLRGVGNAVVFGSQNYSMRLILDPIRMAQLSLTPTDVANVVREQNRDFPA
                     GTIGREPALKGTELTIPVITQGRLTEVKDFEDMIVRAMPNGSMVRLKDVARVELGAQS
                     YTLEGRWNGKPNVFLLTFLSPGANALETVKRVRAELTEVSKAFPTGVSYDIPYDTTRF
                     IDVSIKEVVKTLAEAMVLVILVVYLFLQSWRATLIPGVAVPVSLIGTFAGMQALGFSI
                     NTLTLFGMVLAIGIVVDDAIVVVENCERHMTQGKLSAKNAAKRAMEEVTGPVIAIVLV
                     LCAVFVPVGFLGGITGELYKQFAITISIAVIISGFVALTLSPALCALVLKPGEERHHG
                     LFGLFNRTFSWMQGRYISTVGMALTRRLLSVAVFAVLLTGVVLLFKTIPNSFLPEEDQ
                     GYFITIVQLPDGASKQRTDTVLSKIENYFLANPAIHSTDALSGQNFVFSTRGPNAATM
                     FVPLKHWDERTAPQQHVKALIGAAYGEFAKIPEALILAFNAPSIRGLGATGGFSIQLQ
                     DPSGGDFNKFSAVAQEFVAKARQNPAIGAIGTSFRVSAPRIFAKVNRERAKALGVPIS
                     EVFDTLQAYFGNLYINDFVKFGRVFRVQTEAEAQYRSTPDDISKIYVRAVGAQGPTMI
                     PLDTVVSTEFSSGPDPVTHFNGYNTALVLGSAAPGVSSGQVLDALDKLGKEVLVPKGY
                     GIDWSGISYQERMVGNQSLYAFGFGLLMVFLVLAAQYESWVVPFAVILAVPIGLFGAL
                     SAVWLKGMTNDIYFQIGLVTLIGLSAKNAILIVEFANKRYEEGHPLLEGTIDAAKLRF
                     RPIVMTSMAFILGVVPLVVATGAGAASRNSIGTGVFGGMLAATFLAIFFVPLFFVLIR
                     SLSRGRNRQLPPPGADGPDQPEKERDYQHA"
     gene            complement(10164..11360)
                     /locus_tag="PK589_13245"
     CDS             complement(10164..11360)
                     /locus_tag="PK589_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ20619.1"
                     /translation="MIRRWRWSTIAPMVCGWLALALVPGCKQEAGSAPAPTVPEVGIV
                     VATAQDVPDEPEFIGQAESSRPVEIRSQVTGILKQWYFKEGRDVKKGDRLYQIDPVPF
                     HAAMLSAKAKVAQSEARLVQANQNLTRVKPLLAEQAVSTKDVDDAVAEQLAAKAALEG
                     AKAELVKAKFDLDNTLIIAPIDGMIERTRVYEGRLVSAQTDLLTMIHQVDPMYVIVSA
                     PESFLLKRRRDTDAKRIQHPGVYQLRGVLTFVDGTTYGHEGVLDLLDVGLKTDTGSRQ
                     ARVVFPNPDRVLLPGQFVRVRFKGTLKTGAILVPQRAVQQGAKGSIVFVVGKDDKVEM
                     REILASSWQGSQWIVEEGLHVGDRIIVEGLHKIAPGAVVKPVPVPAAGAATAPSAAPA
                     PPEPAS"
     gene            complement(11748..12347)
                     /locus_tag="PK589_13250"
     CDS             complement(11748..12347)
                     /locus_tag="PK589_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HNJ20620.1"
                     /translation="MRVTSILLACLCTSLMTMPVSAKEKKSEKAMDPQAMMEVWKKAA
                     MPGEPHKLFASLAGSWTTQTKEWMEPGKPPSETTGTAEMKMLLDGRFLYQEFNGTMMG
                     QPFAGIGIDAYDNMTKKYVTAWMDSMGTGIFTMQGTSSPDGKTITLKGSHPEPGGGQM
                     HHRAVWKIIDQNSQTFEMYGTHGHGKEMKFMEITYTRKS"
     gene            complement(12517..12942)
                     /locus_tag="PK589_13255"
     CDS             complement(12517..12942)
                     /locus_tag="PK589_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNJ20621.1"
                     /translation="MRFMIIIKATKDSEAGVMPSQQLLSEMGTFNEELVQAGVMLAGD
                     GLHPSTKGARVRFSGTRRTVIDGPFAETKELIAGYWLWQCGSKEEAIEWVKRCPNPMP
                     GESEIEIRQVFEAEDFGAEFTPELREQEDRLRAQMAQNQ"
     gene            complement(13136..13507)
                     /locus_tag="PK589_13260"
     CDS             complement(13136..13507)
                     /locus_tag="PK589_13260"
                     /inference="COORDINATES: protein motif:HMM:NF018893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HNJ20622.1"
                     /translation="MRYVDGFVLAVPKKNVNAYFKMAKKACKVWTEHGALEYRECVGD
                     DLAVKMGLPFPRLAKLKQGETVVFSWIVYKSRSHRDRVNAKVMKDPRINEMCDEKAMP
                     FDVKRMAYGGFKVMVDGGTRS"
     gene            complement(13550..14017)
                     /locus_tag="PK589_13265"
     CDS             complement(13550..14017)
                     /locus_tag="PK589_13265"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ20623.1"
                     /translation="MSTSHAKPIPDGMHTITPHLICAGAADAIEFYKKAFHAVELGRV
                     PGADGKLLHALIRIGDSPVMLVDEFPDHHTLGPKSIKGSPVTIHLYVKDVDTVFNRAV
                     SAGAKSTMPVSDMFWGDRYGQLEDPFGHRWSVATHVRDVDPQALQEAARKACE"
     gene            complement(14227..14589)
                     /locus_tag="PK589_13270"
     CDS             complement(14227..14589)
                     /locus_tag="PK589_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNJ20624.1"
                     /translation="MKYLLLVHHSEEAFAKMAETTRREMLAESVQLTHQLHAKGQYVS
                     ASPLHPTSTAARVQVREGRAIVTDGPFAETREQTAGYFLVEASGLQDAIDIAARIPGA
                     RLGTVEVRQVREIDGLPE"
     gene            complement(14625..>14921)
                     /locus_tag="PK589_13275"
     CDS             complement(14625..>14921)
                     /locus_tag="PK589_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNJ20625.1"
                     /translation="MVRVILPQHLRTLAKVEGEVSLEVVGAVTQETVLDALEVRYPVL
                     RGTIRDHVTKRRRPFIRFFACEQDVSHDSPDVPLPESVAKGAEPLLIVGAMAGG"
     assembly_gap    14922..15021
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15022..16196)
                     /locus_tag="PK589_13280"
     CDS             complement(<15022..16196)
                     /locus_tag="PK589_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNJ20626.1"
                     /translation="MSRVRVLVGTRKGAFILTSDGTRKQWDVTGPHFGGWELYHLKGS
                     SADPNRLYASQTSSWFGQVIQRSDDGGKTWNPPGTKPEDLMGADGMPNGASNMFVYDQ
                     SAETGKPLTTHQHYDGTQRPWEFKRVWHLEPSLTDPDTVYAGVEDAALFRSTDGGRTW
                     QELAGLRDAKGKLWQPGAGGMCLHTIVQDPANPQRMHIAISAAGVFRTDDGGKSWRPT
                     NRGLKSQFELPDPDAEVGHCVHCITMHPSRPNVLFMQKHWDVMRSDDGGESWHEISGN
                     LPTDFGFPIAVHAHEPNTVYVVPIKSDSEHYPPEGKLRVYRSRSGGNEWEPLTKGLPQ
                     QNCYVNILRDAMSVDSLDPCGLYFGTTGGQVYASADGGDSWAPIVRDLPAVLSVEAQT
                     "
     gene            complement(16284..16640)
                     /locus_tag="PK589_13285"
     CDS             complement(16284..16640)
                     /locus_tag="PK589_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNJ20627.1"
                     /translation="MKYLCLVYVEEKILHALPSAQRQTLSDESMSYCDRLQKLGQLVT
                     ASPLHPVETATTVRVRDGKTSTIDGPFAETKEQLGGFLLLDVPDLNDAIRIASQFPAA
                     RIGSVEVRPMKEQGCA"
     gene            complement(16695..17108)
                     /locus_tag="PK589_13290"
     CDS             complement(16695..17108)
                     /locus_tag="PK589_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ20628.1"
                     /translation="MAKRTPTRKTTKKADCPASLVWFDIPADDLARAKKFYSALFGWK
                     ISRFPAMPDYWHIDTGGEKATLDGGLLPRQAPRQPITAYVSVPSVEKAAAKVQKLGGT
                     VCKSKTPVPGMGYFVICQDTESNTFALWEMNPQAT"
     gene            complement(17247..18497)
                     /locus_tag="PK589_13295"
     CDS             complement(17247..18497)
                     /locus_tag="PK589_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ20629.1"
                     /translation="MTENQSQQVRALVDEVYRTQSRQVLATLIRLLGDFDAAEEALHE
                     AFAVAVEQWAREGVPANPRAWLVSTGRFKAIDGMRRRARFDASLPDLAHHLELTADPV
                     IDEGEDSVEDDRLRLIFTCCHPALSPEAQVSMTLREVCGLTTDEIARAFLTKPTTIAQ
                     RIVRAKGKIRDARIPYEVPSETDFPDRLDAVLRVVYLVFNEGYSASSGDSLTRHDLSG
                     EAIRLGRVLVGLLPEAEVLGLLAVMLLQDSRRAARTSPTGDLILLEQQDRSLWNRDQI
                     AEGMALVELALATRRVGPYTIQAAIAAVHAEAPEAALTDWAQIVGLYDVLLQAEASPV
                     VELNRAVAVAMRDGPSAGLALVDAILSRGDLTNYHLAHSARADLCRRLGRNTDAAASY
                     ERALALTQQEPERRFLEGRLRELG"
     gene            complement(18557..18880)
                     /locus_tag="PK589_13300"
     CDS             complement(18557..18880)
                     /locus_tag="PK589_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNJ20630.1"
                     /translation="MTREKQGAQSDPRVMEALRQVVDPELGINIVDLGLVYGSEVREG
                     QVHVTMTMTTPACPMEELLMEMVHSAILRELSEARSVEVDLVWDPPWKPDMMSPAAKA
                     QLGRT"
     gene            complement(18930..20030)
                     /locus_tag="PK589_13305"
     CDS             complement(18930..20030)
                     /locus_tag="PK589_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20631.1"
                     /translation="MTIRHPRRLPLLALGMVALLTGLWAGVARLGWDVPLPRSGFSSL
                     HGPLMVSGFLGTLISLERAVALGRSWAYAAPLLTGLGALVLIAGGPLVVAQLLLLSGS
                     AGLAAIFAAIIRRHPALYTFTMGGGAIVWAVGNLLWVLGWPVFLVVFWWAAFLVVTIA
                     GERLELARLQQVTRGAQTAFLLFLGVLGAGLLLLGWWFDGAVRLFGAGLAALALWLGL
                     NDIARRTVHQTGLTRFIAICLLSGYAWLGVSGLAAMVYGGVPVGPQYDAMLHAFFLGF
                     VFAMIFAHAPIIFPAVLGARMTYRPVFYAHVLLLQATLMVRLVGDAAGWGAGRQIGGL
                     LNAVTLVLFLANTVTALGSLPDRPVVTSRAGS"
     gene            complement(20053..20595)
                     /locus_tag="PK589_13310"
     CDS             complement(20053..20595)
                     /locus_tag="PK589_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNJ20632.1"
                     /translation="MAEIPAGGVIADFLAEDHRRLDALLQATDAHPGRIDREAYDQFR
                     AGLLRHIGMEEKLLLPALQRWRGGEPLPVAARLRLDHGALATLLMPTPTAQILATIRR
                     ILSEHNRIEEGPDGLYALCGQLPDTEATRLWDALRAAPTVSVMRHSDSPAVMKTLEGA
                     LARAGYRLEALAPREEGESR"
     gene            complement(20657..21055)
                     /locus_tag="PK589_13315"
     CDS             complement(20657..21055)
                     /locus_tag="PK589_13315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNJ20633.1"
                     /translation="MTKLLRITTKQLKEIVDVTELLQATIRNANMKEGLCSVFVTHTT
                     AAVTTGEIGEGTEQDFLQIVEEVIPRIQFRHAHDPSHAWSHMAASMLGPSLTIPVSGG
                     TLVLGTWQSVMLVELDGPRDRNVHVSLMSF"
     gene            21352..22218
                     /locus_tag="PK589_13320"
     CDS             21352..22218
                     /locus_tag="PK589_13320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ20634.1"
                     /translation="MKNLLFPIMMLSLSLAASGCSQATFTAVNLPTRFHEMTVVQDVA
                     YSPEPACTLDIYVPAGAGHQPLDVIVFFYGGRWTEGEKHNYRFVGAAFTEQNFIVVVP
                     DYRKYPRVRFPEFVEDGAKALAWVYDHIAGYHGNPERIHVAGHSSGAHIGALLTADAG
                     YLANEGKDRSRVIRDFAGLAGPYAFTPDEPDLEDMFGPPSNYPNMQVTTFIDGRQPPM
                     LLLYGDADHAVKVANLERLEQRIKEKGGRVETQIYPGAGHRDLAAALTWLNLRRTPVL
                     LDMQRFFRSSVR"
     gene            complement(22257..25481)
                     /locus_tag="PK589_13325"
     CDS             complement(22257..25481)
                     /locus_tag="PK589_13325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ20635.1"
                     /translation="MPMKPVILFVDANSVHVNALREALHGEEAELLCAASSAEAAEVL
                     LTREVVLVVMAARLLQTEGFKCAETLRGMKRTRHVPLILITEAGDDEQLTIPANDAGI
                     LDSLPTPIDPRQWRRKFRMYLEVSRQRQELSQQRMALRTALDEQRGMQAQLRDEEERY
                     RLALTATRDAVWVWNIETDSQVWNQAGADLFGWSEVVEQPQPAQWWVDRVHPDDRERV
                     GGAFHEAIGTPSVTHWADEYRFLKRDGTYAWVLDHGYIVRDVRGSAKRAVGAMQDITE
                     RKRAQEIAAHFAAVVGSSFDAIVSKTPEGVITSWNAAAERMFGYSASEMIDHTAHRLI
                     PVELHAEENDILARISCGESIQHYETVRLTKDGRRVDLSLTISPIRDSKGQIIGSSQI
                     ARDITEQKRQRTALKESEELFRALVDQMSQLAWMMDDTGNVYWFNQRWYDYTGMTIET
                     MHGEAWDQAHHPDHRARVLSSRRRSLETGEPWEETCLLRGKDGQYRWYLTRAVPIRDD
                     QGRIVRWFGTNTDITEHKRTEEDLRTAEQALRQSDTRFRELVERSPFGIYVVDAEFRI
                     AHMNEAGQKGAFCNVRPVIGRDFGEAMRILWPEAVAADIIKQFRHTLETGTPYRSRDF
                     INPRADLQQTEGYEWELHRLTLPDGRHGVICYYYDSTRLRMVEVALRESGERLQAAAA
                     DLEKRVDERTRDLVRSQADLRALASELNLTEQRERKRLAGELHDHLQQMLVFGKLQLG
                     RGMMQTGVDPECMEVIRGVYGMLSEALTYTHTLVAELCPPVLREHGLLAGLRWLADYM
                     KRYGMKVTVKLPDEEDLALPEDCTILLFQSVRELLMNAHKYAGTGLAEVALVHGQGAL
                     MIHVRDRGKGFDSAAATSADLAVGGSSSKFGLFSIKERMRTLGGSFAIASAPGQGTTA
                     TLRLPLTSRAAGAERVGDEKEVPAGTPAVQPNPMGTRPIRVLLVDDHVMIRQGLRAVL
                     EGYSDIELIGEAGNGEEAVALVDRLEPSVVVMDINMPKMNGIEASGRITMRHPAVAVI
                     GLSVNASADNQSAMLKAGARQLLTKEAAAEQLYGAIKSAVGISA"
     gene            complement(25906..>26511)
                     /locus_tag="PK589_13330"
     CDS             complement(25906..>26511)
                     /locus_tag="PK589_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ20636.1"
                     /translation="LAAATARIGVAKADRFPKLSITGILGVASPQLSRLIANETAFGV
                     VGPGLAGPLLNAQILGFQQDAAEAQSRKVLARYEQAVLVAFREVEDALVAVRTSREQR
                     DAQAQQVDALRSALRLANLRYKGGLANYLDVLVAQRNLFEAELALTGTHRLQLVSVVQ
                     LYKALGGGWSPLDMAQQQPGQAPGTRGHALPAAGTSPAGHG"
BASE COUNT         5587 a         8322 c         7113 g         5389 t
ORIGIN      
        1 cgacacccgg atgtccaaac ctacgcagag gaatcaacct catggccgtc cgtgtcgcgt
       61 ccgacctatg ccggcgggct tgggttcggg atgaagtggg atatgggctg gatgcacgat
      121 acgctggaat atatggggct ggatccggtc tatcgcaaac accaccaccg caacctgact
      181 ttccggatgc tctacgcgtt ccaggaaaat ttcttgctcc cgctttccca cgacgaagtc
      241 gtacacggca agggctcgct cttggggaaa atgcccggag acgattggca gaagttcgcc
      301 aacttgcgca cgctgttcgg ctatatgtat gcgcaagcgg ccaagaagct catttttatg
      361 ggtggggaga tcggccaatg gcgcgaatgg gcgcatgacg acagtatcga ttggaatctg
      421 ctcaattatc cgccgcacca aggtttacag cggtgggtcg ccgatctcaa tcatctctat
      481 cgcgctgaac cggccctgca cgagttcgac tttgaccccc gcggattcga atggatcgat
      541 tgccaggatg tcgacgcggg taccatcagc cttctccgcc atggacggtc ttcccgtgaa
      601 agtatcgccg tcgtatgtaa tttcacgccg gtcccgcggc tgcagtatcg agtcggcgtg
      661 ccacagggcg gctattggaa agaattgctg aatagcgatg cctccctata cggcggcagc
      721 ggactgggaa atctcggcgg tctctcggcc gacgcggttc ctgcccacgg tcgatcccat
      781 tcgctcgcgc taaccctgcc ccccctcgcc gtcctgttcc ttaagtctgc ctcgtaaagc
      841 cgactaccaa ctcgcctttc tcctccggct caggtaggat cacggcaggc atcaagcacc
      901 accgaacgaa ctgatccgtt cataagggag ggatgatggc gatacgctca tgcagaacgt
      961 tgatctggca aacaccattg atgctcgtga tagccctctc tcttgcgcat tgctcgcacg
     1021 acatccatga gaaacgcgcc gataccatca aaggtcacgt cgaatcgttt tacgatcatt
     1081 tgaagcatga tcgagtcgct gccgctgttc gtgaaaacga agccatcgag caactctcca
     1141 ctcagctggg agagatcgtg actcggcgtg tcaatcagcc gggcaccaat cagatcgacc
     1201 gtgaatggac ggaccttcgc accgccaatg aaactgccgc gcaaaactgg ctggctctgg
     1261 gacagtatct ttcgatcaag aaacaatatg ctcaatcccg ggccacctac cagcgcgtca
     1321 tcgacaccta taatggcgca accgaacgaa cctatcggga acaggcggcg cgagccattc
     1381 gcgacttgga tattctcagt cctcctcctg cggcccctcg ataaccagaa ctaccgataa
     1441 actcgaacca tgcgattcat tgcatcacgg ctgatgattc ttgtgatggg cctctgcctg
     1501 ctcctgacgg gatgtgtcca cagaatccac gttaatcctg cctcgaacag tcccgccacg
     1561 ataacgattc ctgccgctgt gcaacttgag gtgccgtttc tcgccatcga aggtgctgac
     1621 cacatgccgg gcattgccat gctcgattgg cccgcaaaag atctccgcca agccatcgtt
     1681 cggtactttt ctgaacggca gacctttaga gcgatcggaa cagatcctgc tgaactcaaa
     1741 atggtcgtga aagcctggct cacccttcgc gcaccggatc gttacctgta tagagtccac
     1801 ctggaggccg atctatccca tcccgggcac ggagtcttta agacctatgc tgccgaaggc
     1861 gaggctttgg gatcgtcagt ccggtggatt acggcctcag accaagagcc tattgatgaa
     1921 gccacctctc aggcgctcca acaattggcg acccaactcg aacaggatcg agacctcatc
     1981 atgaacaact tcagccggta gacaggaact ccacttggaa gatctttcac ggcctcacca
     2041 tcgttgacgt ctcgtgcttg gccaggaatc catcgacccg gccgtaagac cagctccatg
     2101 cgagcttcga gttgccgtat ggtcgcggca cgcctgtcct gactttcccg tatacgttta
     2161 gatactgctg cgtatcgcat cagatacgac cctccgcctc ctcaatttct acagagcacc
     2221 tcgcgccacc tgcattgcgc ccaattgagg acctatgcat ttctcgctac cggaaattgc
     2281 aatgcgctcg actggcatta gctttgctta ttccgctgcc tgcggttcaa aacctgtcac
     2341 cactgacgtt tgagtgctcc tgatgaacgg agtcggctca cgcgggaggc ggtttcacag
     2401 cctgcggttg tcgtggagaa agtgcccgcg tgtccaaacg tgtgattctg tgtttcgatg
     2461 gtgaatggga ctgtcctcca gatcccggcg tctggagttt ttcagcgaac cattctccca
     2521 agcagtctcg aactggcgtt caacccgatt cgcattcggc cacaaacgtc taccaactct
     2581 atcaatcaat tctgcctcgt tcaggcgatg ggcggatcca gcagaaatgg tacgaaccgg
     2641 ggacgacaat gccttggttc caccgcttcc gcgacggttc gttcgggtgt ggactggatc
     2701 gaaccattct tcagagctat gcctatctgg cggccaccta tgatcccggc gacgagttgt
     2761 acctgtatgg cttcagtcga ggcgcctata ccgctcgctc tctggtagga ttactcacgc
     2821 tcaccggtct cccagctccg gtgctgctca atgcagacca cgtcagactt gttcgggaag
     2881 cggcaagtcc tcactcaccc tcaggtggtg tcgccgccca tcttcgcgaa tgcctcactc
     2941 gaatggtcac cgatcctgac aaccacgtca tcgacgaggc ctatcgtgtg tatcgaaacc
     3001 tgcagaacgc ctcccagatt gcgactggtc ggcggaaggg gtcttcggac atcaacgtca
     3061 cattgctcgg tctttgggat accgtcggac cgctcggtat tcccacgaaa gcgctcagat
     3121 ggctgaatga acaccggtac aactttcgcg atactgaatt gagtccgatt gtgcggcacg
     3181 cctgtcatgc cctcgctatc gatgaacatc gagcagatca caacgcaaca ctctggactt
     3241 ccccgcccaa agccgcgcag gtgattgaac agcggtggtt tgccggagca catggagacc
     3301 ttggcggaac gtatcctgat cgagatttgg ctgacgtgcc tttagcctgg atacggcaac
     3361 attccatggc caagggatta gctctggagt caggcagaat agacgaaaaa cttaatatgt
     3421 taggccccat tcacgactcg ttcagtgaat gttttgtcgg atttcgaagg tggatccacc
     3481 cgcgattcta tcggcctgtg atgcaaacgg gcacgggcac agaagtctta gatgaatcag
     3541 ttcacactcg gctcctgcac tctcctgcct acagaccgaa aaatgagggg ctgaacaacc
     3601 ttcttacatt ccgctgactg gtgagctgtc gcccttaacg ctcatccctc tacaattagt
     3661 cgggacgcct ccaaagttta aacagcgcct ctgcaacgtc gcttcatcct tccgttggca
     3721 cctagccatc tgcccccctc catcgcagta ccctgtttgc agactatctg atcagacttt
     3781 cacgagatat tctcgccatc gagcggtatg tggcatcacg cgcatacgcg ggagggcaag
     3841 gatgaaacgt tcatcgcaaa caaagcttct cgttcactgc attttgtcgg atgtagccat
     3901 gggctgtgca gaggagggaa aggtatttga tgtggggcag cgcggaactc tacgcacaac
     3961 cttccacaat ctcatcgacg gggatcatct ctccgtgaag attcaactcc gtgacgagcg
     4021 acgaccggtc tccgtccatc tggcaaaagt cagctggata caaggagaac gatttggtgt
     4081 ggaacttctt gttatggacg tcgatgaacg ccttcgcctc aaccgcttcc ttgaagcaaa
     4141 gcttccccta gaactggaat tccaggattc gcacacagaa ctcacaatta gggcggcgga
     4201 ctaaatgcaa catcgctgca attacaacga cttgcatgca aatctcccat taaaaaccga
     4261 tacccaccaa ggggttactc cattcaggtc tatcgaagaa atcatccagg ttctaggctt
     4321 acacgtaggc actgaacaat ggaggaaccc acaatgagct gttttcgttg taacggttgc
     4381 atggtcgaag attttctcct cgatatggaa gattcctcag gaccaatgtg gctccgcgca
     4441 aggcgctgca tgaactgtgg aaatgtgtcg gaaagtgtgt tgcagcgcaa ccggcagtca
     4501 caggaagcga agaatacctg ccttgcggcg tcagggaagc gagaaatcga accttcaggc
     4561 acaacaagcg tagctgaatt ggccgcgtaa acaatcactg acgacctgac accaggaata
     4621 acctagcatg tccattgatg tagagctggc cctcgtattg ggattgttca tgatcggcgc
     4681 ggtggtgctt gccatcgaca acctggtgta tgccttatcc aagcgcttca gtcgatggat
     4741 gcgtaatcga aaatcagtcc cccgcacccc ttcgaccttc acctcgacac agaagaaaca
     4801 attttgatgg accccggaag caacccgcac ggtccttcgc agatgcgagg gaccgacgcc
     4861 atcgcccaga catttcaacc tccttacccc gcaagcatta cgatagattc cctctctgcc
     4921 caagccaaga aggaggcaca catgacgtct tgttcatctg acaaaattgc acgggaaagc
     4981 gcacgaacgc actctgcctc aggcattcct cagaccctcc tcttcttcat cttgaaacaa
     5041 ctggaaacag gtcggcccga cgagcgggtt caacaggatc acgcgcaatg atgatgagtc
     5101 ttaaggctgt ctttcgcgac gcagcgatcg tatacggttg cacatttgca gccgcgttgg
     5161 gcacggctct ccttggagtg aatctgcaga gcaatccgtc aactgcctat atggcgaacc
     5221 tgttagcggg tgcacttggc ttcacagtcg caggcatacg catagttcgc gatcgaacgg
     5281 aacatctggc atgggtggcg gtgatagtct ggattttcaa cttgccaaac attgccctcg
     5341 gcatacaaac gagtacatcg tggattcaca gtggcctcac cctgatcctc atggccgcac
     5401 tgggtggcac gctggccatg attctcacgc ttacgtcctc atctcctcat aggacggatc
     5461 ccgggcgcaa ggaagggccg gcacatcaga ccggctctcg atagaccacc acagacgtgg
     5521 gataattgaa tagaactctg gcagatcgaa ggattggccg cctaactttt ttcaggcggc
     5581 gacgcgatcg gtgaaacagg cagttgccct tcaggtgtcc atccgccacc cagcgctttg
     5641 tacagttgca caatggacac cagatgtaac cgatgtgttc ccatcagtgc caactcggcc
     5701 tcaaaaagat tgcgttgagc aattaagaca tccagataat tcgccaaccc gcctttataa
     5761 cgaaggttag ccaggctcag tgctgatcgt agggcctcaa cctgctgcaa ctgcgcggcg
     5821 cgctgttcac gcaccgtccg caccgccacc aaagaatctt cgacttcctt gaaggccacg
     5881 aggacggatt gttcgtattg ggctaccgcc tgtcttgcct gtgcctcggc agctctctgc
     5941 tgaaatccta ggatctgggc attcaataac ggaccggcca accctggccc ggcaaccccg
     6001 aaggccgtct cgtttgcgac taaccgggac agatgcggac ttgccacacc aagaattccg
     6061 gtaatactca attttggaaa ccgatcggcc ttggccatgc cgattcgtgc cgttgccgca
     6121 gccaggtctt gttcagcctg caaaatatcc ggacgtcgtt ggagcaattc ggaaggaagc
     6181 ccgggcggca cgtccggcgg catgacctgc tcagtaagag agtagccgcg cgcgatacgt
     6241 ccgggattac gcccgagcaa tacactgagc tgattctcct tctggacctt ctgccgctcg
     6301 aactccgcgg ccttcgctgc tgcgttggcc cgttcggctt cgaactgatc ggcatcgagt
     6361 ttggagatca tgccttgccg caagcgagcc tgggcaattc tcaccgattc ctcccaggac
     6421 ttcagtgtgc gccgggcaat gtcgagctgc atatcaaact gcagcaggtc gaagtaggcc
     6481 tccgccacgc cactgaccag ttgcaacaca atggcacgtc gattttcctc ccgcgacaag
     6541 agatcgccgc gcgccgcctc attggaccgt cggatccttc cccaaatgtc gatttcccac
     6601 gatagatttc cttgtaagta gtagttgaac gggttcggaa atccggggaa gagaaaattg
     6661 ctcttacgac cgaagagtgg cgcgtttgcg gtcacattca tttgcggagc atagtcggtc
     6721 ttcgcgataa acaggcgcgc ttgaaattct tcgatggccg ccgcggcgcg tttgaggtct
     6781 ttgttctctt ccaacgccgt tcggataagc ttttgcagtt cctggtcctt cagcagttcc
     6841 caccagggtg tattggcaat cgatgcagcg tcaccgcccg gttcggccat gcggaatgca
     6901 tcgggcgtcg ttgcctcggg acggttgaaa tccggaccaa ccgcacaggc tgctaaaaga
     6961 gtggagaaaa gcaccaatgc caggctacgc atgctgataa tcacgctcct tttcaggttg
     7021 atcaggtcca tcggccccgg gaggaggaag ctgacggttc cgtccgcgac tcaacgagcg
     7081 gatcaaaaca aaaaacagcg gcacgaagaa aattgccaga aatgtggctg ccagcatccc
     7141 gccgaacact cccgtgccaa tggaatttct gctggcagcg ccggcacccg tggcaaccac
     7201 cagaggaaca acgccaagaa taaacgccat tgacgtcatc acgatgggac ggaagcggag
     7261 cttggccgca tcgatcgtcc cctccaacaa gggatgccct tcctcatagc gcttgttcgc
     7321 aaattccacg atcagaatgg cattcttcgc cgacagcccg atgagcgtca ccagaccgat
     7381 ctggaagtag atgtcattgg tcatcccctt gagccacacg gcactcaagg cgccgaacag
     7441 cccgatcgga accgcgagga tcaccgcaaa gggcaccacc cagctttcat attgcgcagc
     7501 caggaccagg aacaccatca acaatccgaa gccgaatgcg tagagcgact gattgccgac
     7561 catccgttcc tgataggaaa tgccgctcca atcgatcccg tatcccttcg gcaccagcac
     7621 ttctttccct aacttatcca gcgcgtctaa gacctgtccg gaactgaccc cgggtgccgc
     7681 cgaaccaagc accagcgccg tattgtatcc gttgaaatgt gtcacgggat cagggccgct
     7741 gctgaactcc gtgctcacca ccgtatccaa gggaatcatc gtaggcccct gcgcgccgac
     7801 ggcccgcaca taaatttttg agatgtcgtc aggagttgac cgatactgcg cctcggcttc
     7861 tgtctgcacc cggaagacac gaccgaactt cacaaagtcg ttgatgtaca gattcccgaa
     7921 ataggcttgc agcgtatcga agacctccga aatagggacc cccaatgcct tggcgcgctc
     7981 ccgattcacc ttggcgaaga tcctcggagc actgacgcgg aaactggttc cgatggcccc
     8041 gatcgccgga ttctgccgcg ccttggccac aaactcctgc gccaccgcgg agaacttatt
     8101 gaagtcaccg ccgctcggat cttgaagctg tatggaaaat ccgcccgtcg caccaagccc
     8161 gcgaatcgac ggggcgttga aggcaaggat caacgcttct ggaatctttg caaattcccc
     8221 gtaagccgcc ccgatcaagg ctttcacatg ttgctgcggg gccgtacgct catcccaatg
     8281 tttgagcggc acgaacatcg tcgcggcatt gggtccgcgt gtactaaaca cgaagttctg
     8341 cccggacagc gcatcggtcg aatggatcgc cggattggcc agaaaataat tctcgatctt
     8401 gctcaatacc gtatccgtgc gctgcttcga cgccccatcc ggaagttgca cgattgtgat
     8461 gaaatatccc tggtcctcct ccggcaggaa actgttgggg atggttttaa acaacaaaac
     8521 cacgcccgta agcagtaccg cgaacaccgc gaccgacagc aggcgtcgcg tcagtgccat
     8581 tcccaccgtc gagatatatc gtccctgcat ccatgaaaat gtccgattga acaggccgaa
     8641 caacccatgg tgccgctctt cgcccggctt gagcacgagg gcgcagagcg cagggctgag
     8701 cgtcaacgcc acaaaaccgg agatgattac cgcaatggaa atcgtgatgg cgaactgctt
     8761 gtacaactcc ccggtaatgc caccaagaaa gccgacgggc acgaacaccg cacacaacac
     8821 aagcacgatg gcgatcaccg gccccgtcac ctcttccatc gcccgcttcg ccgcgttctt
     8881 agccgacagt ttcccctggg tcatgtgccg ttcgcaattt tccacgacca cgatcgcatc
     8941 atccaccacg atgccgatgg ccaggaccat gccgaacagc gtgagggtat tgatcgagaa
     9001 ccctagcgcc tgcattccgg caaatgtgcc gatgagggac accggcacgg cgacaccggg
     9061 aatcaatgtc gcgcgccagc tttgcaaaaa gagatagacc accagaatga cgagcaccat
     9121 cgcttctgcc agcgtcttca cgacctcttt aatggagacg tcgataaatc gcgtggtgtc
     9181 ataggggatg tcgtacgaca cgccggtggg aaatgccttg gagacctcgg tcaattcggc
     9241 gcgaacccgt ttcaccgttt cgagcgcgtt cgcccccggg gagaggaacg tcagcagaaa
     9301 cacattgggc ttgccgttcc agcgcccttc cagggtgtac gattgggcgc ccaattcaac
     9361 acgagccaca tccttcaacc ggaccatgga accattcggc atggcccgca cgatcatgtc
     9421 ctcgaagtcc ttcacttccg tgagacgacc ttgcgtaatg acaggaatcg tcagctcggt
     9481 acccttcaag gccggctcac ggccaatggt tccggcagga aaatcacgat tttgttcccg
     9541 cacgacattc gcaacatccg tcggcgtgag gctgagttgg gccatgcgga tggggtccag
     9601 aatcagccgc atcgagtaat tctgcgaccc gaacaccacc gcattgccta cgccacgaag
     9661 gcgcttcagg ttgtcgagga cccgcagaat ggcatagttg gaaagataga ccgtgtcatg
     9721 cgtaggatca gtcgaactca aggccacgac ggccaacaga tccggcgaca ccttgttgat
     9781 gctgatcccc tgccggacca cttcgggcgg taattgcggc tccgcgagtt tcacccggtt
     9841 ctgggtttgt acctgcgcga tatcgacatc cgtgccgatc tcgaaggtca acttgatcga
     9901 catgtggccg tcgttcgtac tggtggaatc gtagtacagc agattgtcga tgccgggaag
     9961 ttgcacctca atgggccgcg ccaccgagtc ggccaggatt tcggcattgg cgcccggata
    10021 gtcggcttcg atctgcacga caggaggggt gatttcgggg aattgggcaa tggggagggc
    10081 ctgcagtgcc accaagccca gcaccatgat aacgatcgac aacaccgacg caaagatcgg
    10141 gcggtcgata aagaaatgtg agatcacgag gcgggctccg gcggggcggg ggcggcactc
    10201 ggagcggttg ccgcgccggc agcgggaacg ggcaccggtt tcaccaccgc accgggagcg
    10261 attttatgca ggccctccac gatgatgcga tcgccgacgt gcaagccttc ttcgacgatc
    10321 cactgactgc cttgccagct gctcgccagg atttcacgca tctccacctt atcgtccttg
    10381 cccaccacaa atacgatgga gcccttggcg ccctgctgaa cggcgcgttg cggcaccagg
    10441 atggcgccgg tcttgagcgt gcccttgaac cgcactcgca cgaactgtcc tggcaacaac
    10501 acccggtccg ggttgggaaa tacgacgcgc gcctgtctcg atcccgtatc agtttttaat
    10561 cccacatcca gcaaatccag tacgccttcg tgcccgtacg tcgtcccatc gacaaaggtg
    10621 agcacgccac gcagttggta cacccccgga tgctgaatac gtttggcatc cgtgtcccgc
    10681 cggcgcttca acagaaaact ctccggcgca ctgacgatca cgtacatggg atcgacttga
    10741 tgaatcatgg tcagcaagtc tgtctgtgcc gacactagtc ggccttcata cacacgcgtt
    10801 cgttcgatca ttccgtcgat gggcgcgatg atcagcgtgt tatcgagatc gaattttgct
    10861 ttgacgagtt cggccttcgc gccttcgagg gcggctttcg cggccaactg ttcggcgacg
    10921 gcatcgtcca catctttcgt gctcaccgcc tgttctgcca ggagcggctt cacgcgtgtc
    10981 agattctgat tcgcctggac caaccgggct tccgattggg cgactttagc cttggcactc
    11041 agcatcgccg catggaaggg cacgggatcg atttgataca gacgatcccc ttttttgacg
    11101 tcgcggcctt ccttgaagta ccactgtttc aagatgccgg tgacctgcga ccggatctcc
    11161 acggggcgtg acgattccgc ctgtccgatg aactccggtt cgtcaggcac atcctgggcg
    11221 gtcgccacca caattcccac ctcaggaacg gtcggtgccg gcgccgagcc ggcttcctgc
    11281 ttgcaacccg gcacaagcgc caacgcgagc cacccgcaca ccatcggggc tatagtggac
    11341 catcgccatc gcctgatcat gcgtactcct gactacaatc tgaattgccg cgcgggcgga
    11401 gatgattgcg tacccatctt actggttggc tggcatgtaa gcaacgagcc tgttcctgca
    11461 cacacgacgg atcacggtcg gctcggcgct acatcaataa aacatttcgg cagggggatg
    11521 gtgacgatgg ggggactatg agaaatacga tcgccgcata atcagcccac agcgccacat
    11581 gttgcggtga caacacaaag agatggcagc gacagccgga acagaacttc taccatccca
    11641 tcggttgcgg ggatccccat cccaagacac taggaatgag catccccggc agtcatttga
    11701 tgatgggccg atgcggtgat ccgcgtccgc tgcgagaggc gcgagtgcta tgactttctc
    11761 gtataggtga tctccatgaa cttcatttcc ttcccatgcc catgagtgcc gtacatttca
    11821 aacgtctgtg aattctgatc aatgattttc cacaccgcac ggtgatgcat ctgcccgccg
    11881 cccggttccg gatgcgagcc cttcagcgtg atcgttttcc cgtccggact ggacgtgcct
    11941 tgcatggtaa aaatgccggt tcccatgcta tccatccagg ccgtgacgta tttcttggtc
    12001 atattgtcat acgcgtcgat gccgatcccg gcgaagggtt gccccatcat agtgccgttg
    12061 aattcctggt agagaaatcg tccgtccaac aacatcttca tttccgcggt gccggtggtt
    12121 tcggacggcg gcttccccgg ctccatccat tctttggtct gagtcgtcca actcccggcg
    12181 agactggcaa acagcttatg aggctctccc ggcatggccg ccttcttcca cacttccatc
    12241 atggcttgag gatccatggc tttctccgac tttttctcct tagcagagac gggcatggtc
    12301 atgagggacg tgcagagaca ggcaagcaga atggatgtca cgcgcatgat ggcctcctta
    12361 taggtaagat ggcgccgtcg atcccgtggc gccatccgat tcgtcaggtt tctacacggt
    12421 actgtgcaac atctctggat ggcacagcgt ctctgactct agtcaatcac gtggcaatgg
    12481 gctggtcaat gcaccccaga tgaaatcaga ccttgcctat tggttctgtg ccatctgcgc
    12541 gcgcaggcga tcttcttgct ctcgcagttc cggggtgaac tcggccccga agtcctccgc
    12601 ctcgaaaacc tggcgaattt caatctcgga ttcgccaggc atcggattgg gacaacgttt
    12661 gacccactcg atcgcctcct ctttggagcc acattgccat aaccaatagc ccgcgatcag
    12721 ttccttcgtc tctgcaaagg ggccgtcgat gacggtgcgt cgggtgcctg agaatcggac
    12781 gcgcgcccct ttggtacttg gatggagtcc gtcccctgcc agcatcacgc cggcttggac
    12841 gagttcctcg ttgaacgtcc ccatctcgct cagcaattgc tggctcggca tgacgccggc
    12901 ttccgaatct ttggtcgcct tgatgatgat cataaatcgc atggaatcct ccatcaggtc
    12961 agtgtgagca gagatcgacg atgtgtgccg ccttcacaaa gtagtcgtcg gcgcttgcct
    13021 gaatcgacat gggccggctc accctttccg gcaacagatc tccaagtcat ttatcgttgt
    13081 ccatgcagcc aagaaaccgt gagcatgtga tcaagtgatc aatgaggcaa cggcctcatg
    13141 accgtgttcc tccatccacc atcaccttga atccgccata ggccatgcgc ttgacatcga
    13201 agggcatggc cttttcatca cacatttcat taatccgcgg atccttcatc accttggcgt
    13261 tgacacggtc gcgatgactt cgcgacttgt agacgatcca tgaaaaaacc acggtttccc
    13321 cttgcttgag tttggccagt cgcggaaacg gcaggcccat cttgaccgcg agatcgtcgc
    13381 ccacacattc gcggtattcc aatgcaccat gctccgtcca gactttgcag gccttcttgg
    13441 ccatcttaaa atacgcattc acattcttct tcgggaccgc cagcacaaaa ccatcgacat
    13501 agcgcatgtc agatctccat gtggatgtat tgcacgccgt aaaagattcc tactcacaag
    13561 ccttccgtgc cgcctcctgc aatgcttgtg ggtcgacgtc acgaacatgg gtcgcgaccg
    13621 accaccggtg tccgaaagga tcctccagct ggccataccg atctccccaa aacatatcgc
    13681 tgaccggcat cgtgcttttt gcgcctgccg aaacggcgcg gttgaagacc gtatccacat
    13741 ctttgacata cagatgaatg gtgaccggcg accccttgat cgatttcggc ccgagcgtgt
    13801 gatgatcggg aaattcatcg accagcatga cgggagaatc cccgatgcga atcagcgcgt
    13861 ggagaagctt tccatccgct ccgggcaccc gccccaactc caccgcatgg aaagccttct
    13921 tatagaactc aatagcgtcg gcagcaccgg cgcatatcag gtgaggggta atcgtatgca
    13981 ttccgtctgg tatcggcttg gcgtgtgacg tgctcatcgt tgctcctttc gattgaaccg
    14041 ttcccatggt ttgagggaac agtccgtcga gcttcgcgtg tagcccgaga attgaagaaa
    14101 gcattccgcc atcatcggcc tcttcacgcg attgaccttg cccatgtcgg ttacatctgt
    14161 gtagtcggga tcaccccagc ttcgaaccgt cgttgggtgt ctcgcgccac atctcattcc
    14221 ggcaaatcat tccggcaaac cgtcgatctc gcggacctgc cgcacctcca cggtaccgag
    14281 acgcgcaccg ggaatgcgcg ctgcaatatc aatggcgtcc tgaagaccac tggcctcaac
    14341 aagaaaatac ccggccgtct gttcacgtgt ttccgcaaac ggaccgtcgg tcacgatcgc
    14401 ccgtccctcc cgaacctgaa cacgcgccgc cgtggaagtc ggatgcaatg gcgaggcact
    14461 gacatattgc cccttggcgt ggagttgatg cgtcaactgc accgattcgg ccaacatctc
    14521 ccgcctcgtg gtttcagcca tcttggcgaa cgcttcttca ctatgatgca ccagcaacag
    14581 atatttcatg gacctgcctc cacgcatctc tctacaaagc gggattagcc gccggccatg
    14641 gcccccacga tcaacaacgg ttccgcgccc ttcgcgaccg attcgggcaa aggcacatcc
    14701 ggcgagtcgt gtgacacatc ctgctcgcag gcaaagaacc ggatgaacgg ccgacgccgt
    14761 ttggtcacat ggtctcgaat cgttccacgc aacacgggat aacgcacctc cagcgcatcg
    14821 agtaccgtct cctgggtcac ggcccccact acctccagag atacttcacc ttcgaccttt
    14881 gccagcgtcc gcaaatgttg aggcaatatg acgcgcacca tnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnnnnnnnn ngtttgggct tcgacggaca acaccgccgg caaatcccgc
    15061 acaatcggcg cccagctatc cccgccgtcc gccgaggcgt acacctggcc cccggtcgtc
    15121 ccgaagtaga ggccgcaggg atcgagagaa tcgacagaca tcgcatcacg caggatgttc
    15181 acgtaacaat tctgctgcgg cagccccttc gtgagaggct cccattcatt gccgccgctg
    15241 cggctgcgat agacccgcag cttgccctct ggagggtaat gctcggagtc gctcttgatc
    15301 ggcactacgt acacggtatt tggttcatgc gcgtgcaccg cgatcggaaa gccgaagtcc
    15361 gtgggcaggt tcccgctgat ttcgtgccag gactctccgc catcgtcgct gcgcatcaca
    15421 tcccaatgtt tctgcatgaa caagacattc ggacgtgagg gatgcatggt gatgcaatgc
    15481 acacagtggc caacctccgc atcgggatcc ggcaattcaa actgcgactt gagtccgcga
    15541 ttggtcggcc gccagctctt gccgccatcg tcggtccgga acacgccggc ggcgctgatg
    15601 gcaatgtgca tccgttgcgg gttcgccggg tcctgcacga tcgtatgcaa acacatcccg
    15661 ccggcgcccg gctgccacag cttgcccttg gcatcacgca gaccggcaag ctcctgccag
    15721 gtccgtccgc catccgtcga acgaaagagc gccgcatcct cgaccccggc atacaccgta
    15781 tccggatcag tcagggacgg ttccaggtgc cagactcgct tgaactccca aggacgttgc
    15841 gtgccgtcgt agtgttgatg cgtggtgagc ggcttcccgg tttccgccga ctgatcatag
    15901 acaaacatgt tgctcgcgcc attcggcata ccgtctgcgc ccatgagatc ctcaggcttc
    15961 gtgccgggag gattccaggt cttaccgccg tcatccgacc gctgaatgac ctgcccgaac
    16021 caactgctgg tttgcgacgc atacaggcgg ttaggatcag cggatgatcc cttgaggtga
    16081 tacagttccc acccgccgaa atgcggacct gtcacgtccc actgcttccg ggtgccatcc
    16141 gatgtcagaa tgaacgcccc tttcctcgtt cccacaagca ctcgcaccct gctcatatgc
    16201 tgctccttcc tcgattcgca ccggcctgcg gcgcgaggtg atgtcctgac tgcatgacgg
    16261 ggatcacacc ctatcgatac cggtcaagcg caaccctgtt ccttcatggg acgaacctcc
    16321 acactcccga tgcgagcggc aggaaactgc gaggcaatac ggatcgcatc gttcagatcc
    16381 ggcacatcga gcaggaggaa gccgccgagt tgttctttgg tctcggcaaa cggtccgtcg
    16441 atcgtcgacg tcttgccatc gcgaaccctc accgtggtag ccgtttcgac cggatggagc
    16501 ggggacgccg tcaccagctg accaagcttc tgtaaccggt cgcaatagga catggattcg
    16561 tccgaaaggg tctggcgctg agcactcggc agcgcatgca ggatcttttc ttcgacatac
    16621 accagacaga gatatttcat gatctattcc tcgtatgatt caacgttcgc ggatcatgcc
    16681 gcgtgcccct cagtctaggt cgcctgagga ttcatctccc acaaggcaaa tgtgttgctc
    16741 tccgtgtcct gacagatcac aaaatagccc atgcccggca caggcgtttt ggatttgcag
    16801 accgtccccc cgagtttctg cactttggca gcggccttct caacggacgg aacggacaca
    16861 taggcggtaa tcggctgacg cggcgcctgg cgcgggagca gcccgccatc cagcgtcgcc
    16921 ttctcgccgc cggtatcaat atgccaatag tccggcatcg cggggaaccg gctgatcttc
    16981 cacccgaaca gtgcactgta gaacttcttg gctcgcgcca ggtcgtcggc aggaatatcg
    17041 aaccacacca aactggccgg acaatcagct ttcttcgtcg tcttgcgcgt cggggtacgc
    17101 ttcgccatgg catcctccct tatgaatggc tcctgtcatg agatagtcgt cgagggcggg
    17161 gaaaatcgac atccttcgac gcagccatga agctgtcgcg atatcaacca cttaaagaac
    17221 acagagggcg gtcgacacca gtcgcctcaa ccaagttccc gcaatcgccc ctccagaaac
    17281 cgccgctccg gttcctgctg cgtgagtgcc agcgcccgtt catatgaagc tgcggcgtcc
    17341 gtgtttcgtc caagtcgccg gcagaggtcc gcccgcgcgg aatgcgccag atgataattg
    17401 gtaagatcgc cacgcgagag aatggcatcg accagcgcaa gacccgccga cggaccgtca
    17461 cgcatcgcca cagccaccgc ccggttcagt tccactaccg gtgacgcctc ggcctgcaac
    17521 aacacgtcat acaggcctac gatctgcgcc cagtcggtca gcgcagcctc cggcgcttcc
    17581 gcatgcacgg cggcgatggc cgcctgaatc gtatagggac cgacccggcg ggtcgccagc
    17641 gccagttcga ccagggccat gccttcggcg atctggtctc gattccacag cgagcggtcc
    17701 tgttgttcga ggagaatcaa atctccggtc ggcgacgtcc gtgcggcgcg acgggagtct
    17761 tgcagaagca tcaccgccag gagtcccaac acctcggctt ccggaagcag cccgaccagc
    17821 acccgcccaa ggcgtatcgc ttcaccggat aaatcatgcc tggtcagcga atccccggac
    17881 gacgcactgt agccctcgtt gaacacgagg tagacgactc tcagcaccgc atccaaccgg
    17941 tccggaaaat cagtctccga cggcacctca taggggatac gggcgtcgcg gatcttgcct
    18001 ttggcgcgca caatgcgttg cgcaatcgtg gtgggcttcg tcaggaacgc gcgggcaatc
    18061 tcatcggtcg tgagaccaca gacctcccgg agcgtcatgg agacctgggc ttccggcgac
    18121 agggcgggat ggcagcaggt aaagattaat cgtaaccggt catcctcaac cgaatcctcc
    18181 ccctcatcta tgacagggtc agcggtgagt tccagatggt gggcaaggtc aggcagcgac
    18241 gcatcgaatc tggcccgccg ccgcatgccg tcgatcgcct tgaagcgccc cgtcgagacg
    18301 agccaggcgc gaggattggc cggcacccct tcacgcgccc attgttccac tgcgacggca
    18361 aaggcttcat gcaacgcctc ttcggccgca tcgaaatctc cgagcaagcg aatcaaggtg
    18421 gcgaggacct ggcgggactg ggtgcggtag acctcatcca ccagtgcgcg cacctgctga
    18481 gactggtttt cggtcacggt cacaatcctt ctcgagcggg tagaccttgc cgcctcccgc
    18541 actcatgggc tcaccctcac gtccggccga gttgggcttt cgccgccgga ctcatcatgt
    18601 ccggcttcca cggcggatcc cataccagat caacctccac ggaacgtgcc tcggacagct
    18661 cgcgcaagat tgccgaatgc accatctcca tcagcagctc ttccatcggg caagccggcg
    18721 tcgtcatcgt catcgtgaca tggacctggc cttcgcgcac ttcgctgcca tagaccaatc
    18781 ccaaatcgac gatgttgatc cccaactcag gatccaccac ctgacgcaag gcctccatca
    18841 cccggggatc tgactgcgcc ccctgtttct ctctggtcat ccaacactcc cttgtgcgtg
    18901 ctacgaggac gtcttcacta tgatcaacat caggacccgg cgcgactcgt gacgaccggc
    18961 cgatccggca aactgccaag ggccgtaacg gtattcgcaa ggaacagcac caacgtcacc
    19021 gcattcagga gcccgccgat ctgccgtccc gccccccacc ccgctgcgtc accaaccagt
    19081 cgcaccatca gtgtcgcttg caacaacaac acatgcgcgt aaaacaccgg tcgatacgtc
    19141 atccgcgcac cgagcaccgc cggaaagatg attggcgcgt gggcgaagat catcgcaaac
    19201 acgaacccca gaaagaatgc gtgcagcatg gcatcgtact gcggccccac gggaacaccg
    19261 ccatagacca tcgccgccag gccgctcacg ccaagccacg catagccact gagcaggcag
    19321 atggcgatga agcgtgtcag ccctgtttgg tggaccgttc gtctggcaat gtcattgagg
    19381 cccaaccata gggccagagc ggccaacccg gcaccgaaga gacgcaccgc gccgtcgaac
    19441 caccatccca gaagcaacag ccctgcacct aacaccccga ggaacagcaa gaacgccgtc
    19501 tgcgcgccgc gcgtcacctg ctgcaaacgg gccaattcca gacgctcacc cgcaatggtc
    19561 accacgagga aggcggccca ccagaagacg accagaaaga cgggccaacc gagaacccac
    19621 aacagattac cgacagccca gacgattgcc ccgcctccca tcgtgaaggt gtacaaggcc
    19681 ggatgccgcc ggatgatggc tgcaaaaatc gccgccagcc ccgcactgcc actcaacagc
    19741 aagagctggg caaccaccag cggaccgcct gcgatcaaga ccagggcgcc aagaccggtg
    19801 agcaacggcg ctgcataagc ccaggatcgg ccgagcgcca cggctcgctc cagactgatg
    19861 agcgttccaa gaaagccgga caccatcaac ggaccatgca atgaggaaaa cccggatcgc
    19921 ggcagaggca catcccaccc gagacgcgcg acaccggccc agagccccgt cagtaaggcc
    19981 accatgccga gcgcaagcag cggaagccgt cgcggatggc ggatcgtcat cgtgacggtc
    20041 ccttcggcac ggtcatcgag attctccctc ctcacgcgga gcgagcgcct cgagacgata
    20101 tccggccctg gcaagtgcgc cctccaaggt tttcatgacc gccggactgt ccgaatgccg
    20161 catgacgctc accgtcggcg cagcgcgcaa tgcgtcccac agtcgtgtcg cttctgtatc
    20221 cggcaactga ccacagaggg catacaatcc atcaggacct tcttcgattc gattatgctc
    20281 cgacaagatc cgtcgaatcg tcgccaggat ctgagcggtc ggggtgggca tgagcaacgt
    20341 ggccagcgcg ccatggtcga gccgcagcct cgcagccacc ggtaagggct ccccgcctcg
    20401 ccatcgttgc aatgcaggca acaacagttt ttcctccatg ccgatatgcc tcagcaggcc
    20461 cgcacggaac tgatcgtagg cctcgcggtc gatccggccg gggtgcgcat cggtcgcctg
    20521 cagcaaggca tcgagccgcc ggtggtcctc cgcaagaaaa tcggcgatca caccgccggc
    20581 cgggatctct gccatctcgc ctccctctga accagcgcgg ctcatgagcg gccgcctcgc
    20641 atctttcaga cgtctgtcag aatgacatca gggagacgtg cacgttccga tcacgcgggc
    20701 catcaagttc caccaacatc accgattgcc acgtgccgag tacgagtgtc ccccctgata
    20761 ccgggatggt caaggaggga cccaacatcg acgcagccat gtgcgaccag gcatgggacg
    20821 gatcgtgagc atgccggaac tgaatgcgcg ggatgacttc ttcgacgatc tggagaaaat
    20881 cctgttcggt cccctccccg atttcgcccg tcgtcacagc cgcggtcgtg tgggtcacga
    20941 agaccgagca cagcccttcc ttcatgttgg cgttacgaat ggttgcctgg agcaactcgg
    21001 tcacatccac tatctccttc aactgtttgg tcgtgatacg caggagtttc gtcatagagc
    21061 cctcgcctgg cgtcccatgg gagcctcctc attaggacag gttcatacat ctcacactac
    21121 agcggatgca cgacccgtgc aacgatggtt ggcgctgtgc gaggcatcgt tcctgccacg
    21181 gagggtgcgg tatgtcaagg ccatacgctc ccggaagttg cgtggacatg aacttggaca
    21241 tcttcgttgt ggtatgagta ccggaccgcc agtcacatgg ccccttaggc cgaggcgcag
    21301 gcggggtctt ctccgcccgc ccctggggcg tatgaggtcg caaggcacgg catgaagaat
    21361 ctcctgtttc ccataatgat gctgagcttg tctctggccg catccggctg ctcgcaagcc
    21421 accttcacgg ccgtcaatct tcccactcgc tttcatgaga tgacggtggt gcaggacgtg
    21481 gcctactccc cagagcctgc gtgcacatta gacatctatg ttcctgccgg cgccgggcat
    21541 caaccgttgg atgtgatcgt atttttctat ggcggacgat ggacggaggg agagaagcac
    21601 aattatcgat tcgtcggggc agcgtttaca gaacagaatt ttatcgtggt ggtccccgac
    21661 tatcgcaaat atccccgggt ccgctttccg gagtttgtcg aggatggtgc gaaagcgctg
    21721 gcctgggtgt acgaccacat tgccggatat cacggaaacc cggagcggat ccatgttgcg
    21781 ggacattctt ccggagccca catcggcgcc ttgctcacgg cggatgccgg atatctggcc
    21841 aatgagggca aggaccggtc acgggtgatt cgtgactttg ccgggcttgc ggggccctac
    21901 gcctttactc cggatgagcc ggacctggaa gacatgttcg gccctccgag caactatccg
    21961 aacatgcagg tgacgacgtt cattgacggc cggcaaccgc cgatgttgct gttatacggc
    22021 gatgcagacc acgcggtaaa ggtcgcgaat ctcgaacggc tggagcagcg catcaaggaa
    22081 aaaggcggcc gcgtggaaac acagatctac ccgggagccg gacatagaga cctggccgcg
    22141 gccctgacat ggctgaacct tcgcaggacg ccggtccttc tcgacatgca gaggtttttt
    22201 cggtcgagcg tacgatgaca gacggcagac gccatgcgac gcagcaagga gaagactcag
    22261 gcggatattc cgacggcgct tttgatcgcg ccatacaact gttctgcagc cgcttccttc
    22321 gtcagcagct ggcgagcgcc ggcctttaac atggcagact ggttgtccgc gctcgcattg
    22381 acagagagtc cgatgacggc gacggcgggg tgccgcatag taatccgtcc ggaggcttcg
    22441 atgccattca tcttcggcat gttgatatcc atcacaacga ccgacggctc aagccgatcg
    22501 actagcgcaa cagcctcctc gccgtttccc gcttccccga tcagctcgat gtccgagtat
    22561 ccttccaata cggcccgcaa cccctgtcga atcatgacat ggtcatcaac gagcaagacg
    22621 cgaatgggtc tcgtccccat cgggttcggt tgtacggccg gcgtaccagc gggaacctcc
    22681 ttctcatcgc ccacgcgctc cgcccccgcc gcccgagatg tcagcggcag cctcagtgtc
    22741 gccgtggtcc cctgaccggg cgcggaggcg atcgcaaaac ttccgcccaa tgtccgcatg
    22801 cgttctttga tactgaacag tccgaacttt gatgagctgc cgccgaccgc cagatctgcg
    22861 gaggtggccg cagcactatc aaatcctttt cctcgatccc gcacgtgaat catgagcgcg
    22921 ccctgtccat gcacgagcgc cacttccgcc agccctgtcc cggcatactt atgcgcgttc
    22981 atcaataatt cacgaacgga ttgaaacagc aagatcgtgc agtcttccgg gagcgctaaa
    23041 tcttcctcgt ccggcagttt caccgtcacc ttcatgccat accgcttcat atagtcggcc
    23101 agccaccgca accctgctaa cagtccatgc tcgcgtaata ccggcggaca caattctgcg
    23161 accagcgtat gggtgtaggt caacgcctcg gagagcatgc cgtagacgcc gcggatcacc
    23221 tccatgcact ccggatccac cccggtttgc atcatcccgc gccccagctg taatttcccg
    23281 aagaccagca tctgttgcag atggtcgtgc agctcgccgg ccaggcgctt gcgctcacgc
    23341 tgctcggtca ggttcaattc tgacgccagc gcacggaggt ccgcctgcga ccgcacgaga
    23401 tcacgcgttc gttcgtcaac ccgcttctcc aggtcggccg cagccgcttg caaccgctcc
    23461 ccgctctcgc gcaaggcgac ttccaccatc cggagtctcg tcgaatcata gtagtagcaa
    23521 attactccgt gccggccatc aggcagggtg agccgatgca gttcccattc gtagccttcg
    23581 gtttgctgga ggtcggcgcg cggattgatg aagtcgcgcg aacgatacgg ggtgcccgtc
    23641 tccaacgtgt gacggaactg tttgataatg tcagccgcca cagcctcggg ccacaaaatg
    23701 cgcatcgcct cgccgaagtc gcggccgatg acaggccgta cgttacagaa ggcgcccttt
    23761 tgcccggctt cgttcatatg cgcaatgcgg aactcggcat cgacgacgta gatgccgaac
    23821 ggtgaccgct ccaccaattc acggaatcgc gtgtcgcttt ggcgcagagc ctgctccgcc
    23881 gttcgcaggt cctcctcggt tcgtttgtgt tcggtgatgt cggtgttcgt accgaaccac
    23941 cggacgatgc ggccttgatc gtcgcgaatc gggacggcac gggtgagata ccaccgatat
    24001 tggccatcct ttccgcgcag caggcaggtc tcctcccacg gctctcctgt ctcaagagac
    24061 cggcgcctgc tggagagcac gcgtgcacga tggtcaggat ggtgggcctg gtcccaggcc
    24121 tcaccgtgca tcgtctcgat cgtcatgccc gtatagtcat accatcgctg attgaaccag
    24181 tagacgttac cggtgtcgtc catcatccag gccagctgcg acatctggtc gaccagggcg
    24241 cgaaacagtt cttcgctttc cttgagcgcg gtacgctggc gtttctgttc cgtgatgtcc
    24301 cgcgcgatct gagaggatcc gatgatctgc cccttggaat ctctgatggg ggaaatcgtc
    24361 aaagaaaggt ctacccgccg gccgtctttc gtcagcctga ccgtttcgta gtgctggatg
    24421 gattccccgc atgaaatgcg cgcgaggatg tcgttctcct cggcatggag ctcgaccgga
    24481 atgagccggt gcgccgtgtg atcgatcatt tccgatgcgg agtacccgaa catccgttca
    24541 gccgcggcgt tccagctcgt gatcacgcct tccggcgtct tgctgacaat cgcatcgaac
    24601 gacgagccaa ccacagccgc gaagtgcgcc gcaatttcct gggcccgctt acgctcggta
    24661 atatcctgca tggcgccgac cgcacgcttc gccgagccgc gcacgtcgcg gacgatatac
    24721 ccatgatcca ggacccacgc ataggttcca tcccgcttca ggaagcgata ctcatccgcc
    24781 caatgcgtca ccgacggggt gccgatggcc tcgtgaaacg ccccacccac acgttcacgg
    24841 tcgtccggat gaacccggtc tacccaccat tgcgcaggtt gtggctgttc gacgacctca
    24901 ctccaaccga acaaatcagc gccggcctga ttccacacct gactgtcggt ttcaatgttc
    24961 cacacccaga cggcatcccg cgtcgcggtt aatgccagcc ggtaccgttc ctcctcatcc
    25021 cgcaactgcg cttgcatccc tcgctgctca tcgagagccg tgcgcaaagc catgcgttgc
    25081 tgagacaatt cttgccgctg ccggctgact tcgagataca tcctgaactt tctgcgccac
    25141 tgacgcggat cgatcggcgt aggcagggaa tccagtattc ccgcgtcatt cgcgggaatc
    25201 gtcagctgtt catcgtcacc ggcttccgtg atgaggatga gcggcacatg gcgcgtacgt
    25261 ttcattccac gcagcgtttc cgcacatttg aatccctccg tctgtaacag ccgcgcggcc
    25321 atcaccacga gcaccacctc acgtgtcagc agcacttcgg ctgcctcagc agacgaggcg
    25381 gcacacaata gttcggcctc ttcgccgtgc aatgcctcgc gtaacgcgtt cacatgaacg
    25441 ctgttcgcat ccacgaacag aatgacgggc ttcatcggca tagcgggtac gtcctcctga
    25501 gtgtcatctc tcgttgggga gcctgtgcca ttacccgcgc gatggccccc tctatcgaga
    25561 catcccggca tgtagcaccc ggatggtggt tgtggaaaac ggcgtctctg cagaaccacg
    25621 caattcgccg ttccacatca agggcatagc ccctggtcga ctgtgctcaa tctggactca
    25681 gaaaacgcct actccttggt cgaacctaag aaaagttcgg taggcaaagg ggaggatgga
    25741 gtatacaccg gctgaaatat ggaagcgaat ttcctgcggt ccgccccgcg gtcaccgtac
    25801 agagggagaa tcagacggag cagtgctatg acaaagcgac gaccaccaga ctgaatcagg
    25861 cagaggtgac actctggcag aaggtgagcg agccggccga cgccatcatc cgtgaccggc
    25921 cggtgaggtg ccggcggccg ggagagcgtg ccctcgcgtt cctggagcct gccccggttg
    25981 ctgttgcgcc atatccagcg gcgaccatcc cccaccgaga gccttgtaga gctgcaccac
    26041 cgagaccagt tgcagccggt gggtacccgt caatgccagc tccgcctcga acaaattgcg
    26101 ttgcgcgacc agcacatcga gataattggc cagccctccc ttatagcgaa ggttggccag
    26161 ccgcaaggcc gaccgtaagg catcgacctg ctgtgcctgc gcgtcccgtt gttcccgcga
    26221 cgtgcgcacc gccaccagcg catcctctac ttcgcgaaac gcgaccagca ccgcctgttc
    26281 ataccgggcc agcactttcc ggctctgagc ctcggcggcg tcctgctgaa agcccagaat
    26341 ctgcgcgttc agcaacggtc ctgccaggcc ggggcccacc accccgaatg ccgtctcgtt
    26401 ggcgatcagc cgcgacaatt gcgggctcgc cacgcccagg atcccggtaa tactcagctt
    26461 cgggaatcga tccgccttgg cgaccccgat cctcgccgtg gccgccgcca a
//
LOCUS       DAOQDS010000053        25991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_672_length_25991_cov_19.279766, whole genome shotgun sequence.
ACCESSION   DAOQDS010000053 DAOQDS010000000
VERSION     DAOQDS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 25991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25991
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_672_length_25991_cov_19.279766"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /gene="coaD"
                     /locus_tag="PK589_13335"
     CDS             complement(<1..287)
                     /gene="coaD"
                     /locus_tag="PK589_13335"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNJ20637.1"
                     /translation="MKTAVYPGTFDPITHGHSDIIRRGFRMFSRVIVAIAPNPGKHPL
                     FTTKERLEMVRLVTKDLPNLEVTTFEGLLVDFVRTSGAHAILRGLRAISDF"
     gene            complement(284..856)
                     /gene="rsmD"
                     /locus_tag="PK589_13340"
     CDS             complement(284..856)
                     /gene="rsmD"
                     /locus_tag="PK589_13340"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNJ20638.1"
                     /translation="MRVIAGLHRGRRLLGPRGQAIRPTSDRVKEALFSILGERIVGAR
                     VLDLYAGTGSIGIEALSRGAAHVTFVEADREALRLVQSNLRQCGLEQSANVCACQVSQ
                     FFRRGTQWSGPYDVVFCDPPYQLTPELITLAAEWRAGWLAENAIVILEHGRKADVPSI
                     LGPLSQVKRYDYGDTALTRFHLTPKEVHPA"
     gene            complement(1164..1337)
                     /locus_tag="PK589_13345"
     CDS             complement(1164..1337)
                     /locus_tag="PK589_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20639.1"
                     /translation="MECPKCKGMMMLERFSDFFLIFYAWKCINCGAIIDRTISANRRK
                     SLAARDAQPVATR"
     gene            1522..2238
                     /gene="radC"
                     /locus_tag="PK589_13350"
     CDS             1522..2238
                     /gene="radC"
                     /locus_tag="PK589_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNJ20640.1"
                     /translation="MTPKKTGRGIGDWPKTERPRERLLSEGAESLSDAQLLAILLRVG
                     RQDASAVNVGMEVLGRVGGIVGLLHCSTEELCAIPGVGPAKAAQLKAAVEVGKRAVSA
                     PLTTGTRINSSADLFKHYHARLRDLRHEIFAVVLLDAKNQVIRDVTISEGSLTLSIVH
                     PREVFIPAMRASAAGVIFLHNHPSGDPTPSQEDRVLTARLVSAGALLGIQVLDHLIVG
                     DGRYVSFADQGWLGGEASKA"
     gene            2349..4457
                     /gene="fusA"
                     /locus_tag="PK589_13355"
     CDS             2349..4457
                     /gene="fusA"
                     /locus_tag="PK589_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNJ20641.1"
                     /translation="MMNVPLTESIRNVAVVSNAGSGKTSLVEALACCAGSLSAPGSIQ
                     AGTTVSDFEPEEIHHRTSLHTSVIRCLYNDHVLNLLDTPGSPSCMAELRAALRVADGV
                     VLVVNAATGVRSDLERLWSFIREAGLPCMVFLNDLDREGTTFATTLEEVTKALDMPAV
                     PLILANGEGGQLGGIVDLLQNCVVTGQADRFQAGPSPIPPEWRDRIDQARRGLIEMVA
                     EGDEALLERYLNDGTLTDAALLEGLRVGVQRGALVPVFGGSALRNVGVQTLLQGLLQL
                     MPSPAARAQIAPVQGVGLSDGEPPMTREPVATAPLSGLVFKTIIDPFVGRLSYVRIYS
                     GTLQADSNVYNATRQVRERGGHVFSIFGKKYTAVSRAVAGDIVAIGKLKDSLTGDTLC
                     DEHAPIRLPGIPLGRPVMSFAIEPKSKADIEKVSVGLHKLIEEDPSLEFVRHMETKEM
                     VLSGMGQLHIDVALEKLHRKYGADVTLHAPKIPYRETIRSVAQAQGKYKKQTGGHGQY
                     GDCWVEVGPLPRGQGFEFQNKIVGGAIPRNFVPAVEKGVVEALHEGPLAGYPAVDVRV
                     SVYDGSYHVVDSSEMSFKIAGSMAVKKAMEAAHPVLLEPLMTVEVDVPAECVGAVLGD
                     LNGRRGRIVSVEASGHIETVKALVPQAEMVSYAASLNSLTGGQGSYVMEFASYEEVPR
                     ELASRIVDEHKAERNGVAAH"
     gene            complement(4503..5906)
                     /locus_tag="PK589_13360"
     CDS             complement(4503..5906)
                     /locus_tag="PK589_13360"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNJ20642.1"
                     /translation="MKTPLSCSTLLCLGLILTGSFLGSPLSAVAAADVASTPRTVSPG
                     LRLLEELQTVITDLAEQAKPSVVSIFPIQATGRSRDGGERVPSSTGSGSGVIVDAAGH
                     IITNNHVVGDAAEVEVRLSDKTKLFAQVIGKDPDTDLAVLKVTADHPLPAAHFGDSSG
                     VKVGQWVLAVGNPFGLDRTVTLGVVSGIGRENINLSRYENFIQTDASINPGNSGGPLF
                     DLRGDIIGINTAIINFAQGIGFAIPSNMAKQVMNQLIAKGKVVRAWLGVGLQPLTPEL
                     ASKFGVDENEGVLVNEVFERDPAALAGIKPGDVITKVDGALVDTPNKLSRLVAALDPG
                     STTRVEVVRDGKRLNLQVALSERKDTVVTASLPQSRTDLKLGIDVQDLTAGLAEKFRL
                     NESRGVLISKVDAGSLAQAEGLREGDLIKEVNRVDTGTVGEFTVAVAKVKRGDTILLR
                     VLREGRAFYVVLKPADH"
     gene            complement(5996..6634)
                     /locus_tag="PK589_13365"
     CDS             complement(5996..6634)
                     /locus_tag="PK589_13365"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNJ20643.1"
                     /translation="MLSFELLARIRKELFLTGSALYETIVAIAERVNRKVHVLRLHGQ
                     ATTILNQTHAIHRQIGQRVAALAAKPAAGIHPVAPSELASLQDAIRTASDRIKGQQAA
                     LQLVERQIRELKVELAHEDLLTMQRELGLRDAAIERVVVARGAPVIGHPIGEFDLPPT
                     TRIAAVFRGPFLLPLSHTMTLRPDDIVILVGLRHDLAHWLPHFQHPAASKSA"
     gene            complement(6664..7266)
                     /gene="plsY"
                     /locus_tag="PK589_13370"
     CDS             complement(6664..7266)
                     /gene="plsY"
                     /locus_tag="PK589_13370"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNJ20644.1"
                     /translation="MDSPWLIGVLIIFAYLLGSVPFGVVVSRLLGAVDPRTAGSKNVG
                     FTNVLRVSGKKAGVLTLAGDIGKGWLAGWVATMVLQQETAILGVALASILGHLHSIFL
                     GLKGGKGVATALGAALGVAPWVGLTLMTLWIGAVFLWKYSSGGALFAFGMFPIVALIF
                     HRSWTFIGFACVVSVLIWMRHKDNLIRLWSGTESQIGQSS"
     gene            complement(7296..7910)
                     /gene="pgsA"
                     /locus_tag="PK589_13375"
     CDS             complement(7296..7910)
                     /gene="pgsA"
                     /locus_tag="PK589_13375"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNJ20645.1"
                     /translation="MGENWKEAGLVLMRSAGQESNINLPNVLTLVRILLIPVFVMLLI
                     DPTPDRALSAAIVFVVAAVTDLLDGYVARKTGQITKLGRLLDPIADKLLVLSALILLV
                     QVDRVSALVAILIIAREVAVTGLRAIAASEGLIMSAEVTGKYKMALQVIAIVLLVLEG
                     TVVETIGNLHLAGIVTLYLSLILGYVSGAQYVWSFWRQVGAKGL"
     gene            complement(7953..8990)
                     /locus_tag="PK589_13380"
     CDS             complement(7953..8990)
                     /locus_tag="PK589_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNJ20646.1"
                     /translation="MRRSRSTTSPVTRKTKTSAGRSAAVDASVREGIRVLDIEARAVL
                     DLKARLDERFARAVTLLYECQGKVVISGMGKSGLIGQKIAATLASTGTPSFFLHPAEG
                     VHGDLGMLARRDVLIAISNSGETQEVLQLLPFVKRMNVPVVGMTGKMTSTLSKNSDVT
                     LDVSVDEEACPLGLAPTASTTATLAMGDALAVALLQKRGFKHDDFAQFHPGGSLGRRL
                     LVKVRDLMQHGDHLPRVRADVSGADTILEMTSKKLGLTTVVDAKGSLYGIVTDGDLRR
                     FIQAGGDFSRVTAGDLASRHPKTIGPDELATTAVAQMERFSITALVVIEPPNRLAGVI
                     HLHDLLKHGIV"
     gene            complement(8957..9790)
                     /gene="kdsA"
                     /locus_tag="PK589_13385"
     CDS             complement(8957..9790)
                     /gene="kdsA"
                     /locus_tag="PK589_13385"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNJ20647.1"
                     /translation="MAYEVDLGQFKIGAGSRPFLIAGPCVIESEQLVMDTAGRIAEIT
                     KSLGMPYVFKSSFDKANRTSISSFRGPGLEKGLAVLAKVKQQIGVPILTDVHSEEQAT
                     EAGHVVDMLQIPAFLCRQTDLLIAAAKTGKVVNIKKGQFLSPPEMGNAVKKVEDAGNR
                     RIVLTERGSSFGYNNLVVDMRSFPIMRRFGYPVVFDATHSVQLPGGGGTKSSGQREFV
                     EPLACAAAGAGCDGFFMEVHPDPDSALSDGPNMVPLHALHSLLERVLRICDAAAPQPV
                     R"
     gene            complement(9934..11535)
                     /locus_tag="PK589_13390"
     CDS             complement(9934..11535)
                     /locus_tag="PK589_13390"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNJ20648.1"
                     /translation="MSKLIFVTGGVVSSLGKGLASASIGNLLESRGLKITFLKLDPYI
                     NVDPGTMNPYQHGEVYVTEDGAETDLDLGHYERYTSLTLTRENNYTTGRIYHSVITKE
                     RRGDYLGGTVQVVPHVTDEIKQCIMRTSQGMDVTIVEIGGTVGDIESLPFLEAIRQLP
                     YDVGRDNVLYVHLTLVPYIGAAGELKTKPTQHSVNKLREIGIQPNILLCRTDRYLPPE
                     LKAKIAMFCNVEKDAVITAKDVETIYEVPIVFRKEGLDELIVRQLKLETGPPNLREWD
                     AMVQKIKHPKHEVSIALVGKYAGLKECYKSLAEALVHGGIDHETRVNVTWIESEDVER
                     QGTERILREADGILIPGGFGSRGIEGKIVTIQYAREHQIPFLGLCLGMQCATIEFARN
                     VAGLTGANSAEFDAQSPHPVIHLMSDQQSVNDKGGTMRLGAYACTLGEGTLAQKMYGV
                     SEVRERHRHRYEFNNAYREQLTAKGLILSGLSPDGRLVEIVELQNHPWFLATQFHPEY
                     KSRPHHPHPLFSGFVGAALRRKCGH"
     gene            complement(11567..12337)
                     /gene="kdsB"
                     /locus_tag="PK589_13395"
     CDS             complement(11567..12337)
                     /gene="kdsB"
                     /locus_tag="PK589_13395"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNJ20649.1"
                     /translation="MKRSVTVVIPARYGSSRFPGKPLIDLMGKPMIQHVYEQAKACRV
                     VDDVLVATDDDRIKRAVEGFGGQVLMMTEPYRTGTDRVAAVAKSRGGDCYIDLQGDEI
                     LLHPDLITDLVEPFLASDAPMGTLKRRIDSDQDLQNTGVVKVTTDREGNALYFSRAPI
                     PLVRDDPKRSAVPGLHFIHLGLYIYTRETLASLTALPTGILEDAEKLEQLRALENGIR
                     IRVWETSQMSLRIDSPEDVPVALTQLRAHTSRAGSTQP"
     gene            complement(12339..13319)
                     /gene="rfaE1"
                     /locus_tag="PK589_13400"
     CDS             complement(12339..13319)
                     /gene="rfaE1"
                     /locus_tag="PK589_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HNJ20650.1"
                     /translation="MDHAGLGAYIQRFSRASVLVIGDLILDHYVWGRVSRISPEAPVP
                     VVHVESESLKLGGAANVFNNILALGGQADICGVIGADESGRLLLKELGGRRQGRGGVV
                     IDQSRPTTRKSRVVAHNQQIVRYDVERRNELSSLLQRRILRYVESRLKELSCLVVSDY
                     AKGVVTASLMTELTRLAGQRKIPIVVDPKVEHFSYYKGVTVVTPNHLEATQAAGVQGE
                     DDKTINKAGMVLRQRLGCQTVLVTRGERGMSVYQGHGDHWHIPTRARQVYDVTGAGDT
                     VVGTLALALSTGASMREAAILANQAAGVVVGMVGTATVTAAQLADALGQS"
     gene            complement(13373..14152)
                     /gene="lepB"
                     /locus_tag="PK589_13405"
     CDS             complement(13373..14152)
                     /gene="lepB"
                     /locus_tag="PK589_13405"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNJ20651.1"
                     /translation="MSVDPNARPEEIPATNAPAPPPTPPEPATTEPRLVVANQPAHKS
                     ILREYAEAIIIAMLLAFAIRVFVVQAFKIPSGSMIPTLLVGDHILVSKLSYGLQWPTD
                     CKFQPGFPPVTCYSSHTVIPFGSIQRGDIIVFRFPEDEDKDFIKRVIGLPGDTIHVRN
                     KVVHINGTPFDDHAFTQHTDPPIHDGHISPRDNFGPVTVPEDAYFVMGDNRDHSLDSR
                     FWGYVRTEKVRGKAFRIYWSWSGQGSWTEWVRWERLGKAIQ"
     gene            complement(14149..15948)
                     /gene="lepA"
                     /locus_tag="PK589_13410"
     CDS             complement(14149..15948)
                     /gene="lepA"
                     /locus_tag="PK589_13410"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNJ20652.1"
                     /translation="MQSLIRNFSIIAHIDHGKSTLADRLLDATGAVTAREAKEQILDA
                     MDLERERGITIKAHAVAIRYKAQDGKTYLLHLIDTPGHVDFTYEVSRSLAACEGSLLL
                     VDATQGVQAQTIANVNLAMGNHHTIIPVINKIDLASADVEGTKQQISDVLTLDATDSM
                     LVSAKEGLGVPEVLEAIVKRIPPPSGDPTRPLKALIFDSWFDNYQGVIVLTRIIDGSV
                     RPGMKIKVMSNDRLFEVTEVGQFTPKRTKGTQLLTGEVGYLCANMKEVADVKIGDTLT
                     DAARPTDQPFPGYKEVKPLVFCGLYSTDTARYEDLRDALLKLRLNDSSFIYEPETSLA
                     LGFGFRCGFLGLLHMEIIQERLEREYGLTLITTAPTVIYRILTTKGDVLELNNPAELP
                     EPSSIASFEEPFILATLIAPERYLGTLLQLCQERRGIQRSIHYLDPTRVVIAYELPLN
                     EVILDFYDKLKSKTQGYASLDYELLGYRESELVRLDILLNGEPVDALSFITHKERAYQ
                     RGRQVAEKMKELIPKQMFEIAIQAAIGNKIIARETIGAIKKNVTAKCYGGDISRKRKL
                     WDKQKEGKKRMKAVGRVEVPQEAFLAILKVGEE"
     gene            16104..17486
                     /gene="bioA"
                     /locus_tag="PK589_13415"
     CDS             16104..17486
                     /gene="bioA"
                     /locus_tag="PK589_13415"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNJ20653.1"
                     /translation="MTPPSTRQPLAAWDHAYVWHPFTQMQEWEQESPLIIEKGKGSYL
                     IDTEGRKYLDGTASIWVNLHGHRHPRLDRALTAQLRQIAHSTFLGLTNPPAIRLAREL
                     IRLAPKGLRRVFYSDNGSTAVEVALKMAVQYWQQARPSNGSKQSFAHLKMAYHGDTIG
                     AVSVGNIELFHGRFKPLLFPTHQVEPPYCYRCPLGRAYPSCDMACIDPLERLLKTRHR
                     ELAGVIVEPLVQAAAGMMVAPPGYLARVRELCTKYNVLLIVDEVATGFGRTGKMFACQ
                     HEGVTPDLMAISKGLTGGYMPLAATLTTDAIYRAFLGRYDEWKTFFHGHSYTGNPLGC
                     ALALANLEIFRRERTLAQVRKKSRLLARLLQPLAELPHVGDIRQCGFMVGIELVQDRQ
                     TKTPYPLEWRIGHRVAQACRLRGLLLRPLGNVMALVPPLSISPRELTTMIAILHSAIR
                     EITEELATSR"
     gene            17661..18887
                     /locus_tag="PK589_13420"
     CDS             17661..18887
                     /locus_tag="PK589_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20654.1"
                     /translation="MTTGLIIRVLCLLLFLPFSLWAEDQQPSELLLQRIQFSDTARES
                     GTAAIPFSIASPVMHTSFQATSFAPPTTSTRLKDLVPDSERPQTKGLGANSTWLKGQL
                     TAEGEVANNATNDAGLTSRIDQRDDGAKRMVRMALTGTNGPVRYGINYRSAGKAFFNS
                     PDQTVREMWAEWGLGIAKLRSSSGQTWNNVDLEPNRPRIQQDVSRIGIALVKPAWPEL
                     SLTYARSALASSYIPAGMTAQRSQSNSMEAALAYTGLSWNARLSSSYILTNDEMRGGA
                     QSTTFAQTLVASYRPLNTLTFAPTFSYRTETQSWSGAKIQTPMASVSLLYKQSQRLLV
                     SAMGGYTSTKSSDGLIETESIVGRGMFAFHLNPIYGHPTTLSLEASYTNTTNRTVAGV
                     DTEDLSGLVRILVAAL"
     gene            complement(18916..20064)
                     /locus_tag="PK589_13425"
     CDS             complement(18916..20064)
                     /locus_tag="PK589_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ20655.1"
                     /translation="MKPLLQHLSVVLLCLTVMGCTRPDPPAPSAKPVQPSVVANGQPD
                     IQTMRIDASPSLPALTLSARVTYAEDGYSKISSPLPGPVLDVRVKLGQAVKAGDILMV
                     IDAADIARAYAAYVEEISELGLAERNYELTKDLYDAQAMSLKDLEHAENDLNRERAEF
                     KQAKELLLSLRVPAAELSKPLAQQQITSRFELRSPLTGTVVERNVTPGQIVGTASDQP
                     LFTVANLDRLQVVADVYEHDLSGIRVGALAAMTVEAYPAIEFPAKIAVIGDVVDPATR
                     TIKIRATVSNQDRRLKPEMFARLTIAGHTMRPKIVIPKQAVMERSGRQWIVVELAPGR
                     FEERPVTIDSVTDNQVTIREGLVTGERIVLSPSSINHLAASDTSAGGA"
     gene            complement(20406..21206)
                     /locus_tag="PK589_13430"
     CDS             complement(20406..21206)
                     /locus_tag="PK589_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNJ20656.1"
                     /translation="MPVTIPLITLLTDFGDRDYFVASVKGVILNINPQAHIVDLSHHV
                     TPHDVADAAYLLKSCYRYFPDGTIHVAVVDPGVGTTRRPLLVSSSRYFFIGPDNGLFT
                     HIYQEETGVEVRHLENRQYRLDSEGATFDGRDLFAPAAAWLTKGQPIGSFGRLVPNYE
                     RLPISEPSWDKHVMAGEIAYIDRFGNLISNLTAYHIREVRGITKRADPYIRIGGVTID
                     CLVHSYGEGSADAPQALINSNGYVEVFLKEGRAADRLNVARGARIELS"
     gene            complement(21286..21855)
                     /gene="greA"
                     /locus_tag="PK589_13435"
     CDS             complement(21286..21855)
                     /gene="greA"
                     /locus_tag="PK589_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNJ20657.1"
                     /translation="MSPPHGSWMRATGRYRKVQPEAGFNGHRGEDMPTPITRKGYEAL
                     KAELDRLHKVERPRVIEAIAEARAHGDLSENAEYDAAKERQGFIEARLAELKGKLADC
                     RIIDIAGRTSDTVVFGATVVLIEQEAQAKKQYTLVGQDEADLKFSRISVQSPVGRALI
                     GKRVGDLVEVTTPAKVVEYEVMEIRFEEL"
     gene            complement(21905..25174)
                     /gene="carB"
                     /locus_tag="PK589_13440"
     CDS             complement(21905..25174)
                     /gene="carB"
                     /locus_tag="PK589_13440"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNJ20658.1"
                     /translation="MPRRTDIRSILLIGSGPIVIGQACEFDYSGTQACKALKEEGYRV
                     ILINSNPATIMTDPDFADRTYIEPITLDVVEKVIEYERPDALLPTMGGQTALNTAIGL
                     AKRGVLEKYNVKLIGASIEAIHKAEDRDAFRQAMWKIGLRVPDSGVATSLQEAEEVVR
                     RTQFPAIVRPSFTMGGTGGNIAYNIEEFRTQVEWGLAMSPVRQVLVEQSVIGWKEFEL
                     EVMRDLKDNVVIICPIENLDPMGVHTGDSITVAPALTLTDKEYQMLRDAAVRIIREIG
                     VDTGGANIQFGMNPVNGDMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     ITNDITGVTKASFEPTIDYVVVKIPRFAFQKFPGADPTLTTQMKSVGEVMAIGRTFKE
                     SLQKAIRSMEVDQFGFSSKMGLDLGVPPSLNREEAAEQVRKAVRTPLPDRLWRLADGM
                     RLGMTNQELFALTKIDPWFLEQIREIVEFEPRIVAERGALGDAGLRPELLVEAKQLGF
                     ADERLAQLLGVPQSTVRRWRLALGQGNTPRSVTYKRVDTCAAEFEAHTPYLYSTYERE
                     CEARPTTRKKVVILGGGPNRIGQGIEFDYCCVHAAMALREEQVETIMVNCNPETVSTD
                     YDTSDRLYFEPLTEEDVLNIVEREQPMGVVLQFGGQTPLKLALSLSRAGVTILGTSPD
                     AIDRAEDRARFRELLDKLGLRQAESGMAHSVDEALKIAAEITYPVMVRPSYVLGGRSM
                     QIVYDEAGLLHYMNSAVKASAQHPVLIDKYLRDAIEIDADAISDGTTVVVAGIMEHIE
                     EAGVHSGDSACSLPPYTLDSATIEEIRRQMTALALELGVIGLMNAQFAVKDQTIYVLE
                     VNPRGSRTVPFVSKAIGVPLAKLAMKVMVGKSLQQLNFTTAPMPTHLSVKEAVFPFTK
                     FAGVDVLLGPEMKSTGEVMGIDSDFGWAFVKSQAGAGAILPTSGTAFLSVKSEDRAVA
                     CDVAQRLVALGFRITATSGTAAYLTEQGMQVDVVNKVQEGRPHIVDHIKNGDVALVVN
                     TVRTASGHSDSLSIRREALHKGVPYYTTMRGALAAVMGIEALLKKGLAIRALQEYHRV
                     N"
     gene            complement(25197..>25991)
                     /locus_tag="PK589_13445"
     CDS             complement(25197..>25991)
                     /locus_tag="PK589_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNJ20659.1"
                     /translation="FIYLSEEKEMAMGLSAFREVLRQAPLSDNPELNEMVHRVGNRIA
                     KAANKPEYQWEFAVIQDDRTINAFALPGGKVAVFTGILKVTKTEDGLATVIGHEVAHA
                     LQRHGAERMSRGILEQIGQLAALGAGAAVGRPDAAMAAMTVYGVGVSLPFDRRQESEA
                     DFIGLRLMAEAGYDPREAVSFWERMSGCPRAMINKLCFRSQQAIPEFLSTHPSDVTRI
                     NQIEAWIPDAMKHYHPAGKAPAIPSGPIQPYRPPVGPMPEAPLPTG"
BASE COUNT         5115 a         7616 c         7814 g         5446 t
ORIGIN      
        1 tcgaaatcag aaatcgcgcg cagtccccgc agaatagcat gggcgccgct ggtgcgcaca
       61 aaatccacca acagtccctc gaaggtggtg acctccaggt tgggaagatc tttggtgacg
      121 aggcgcacca tctccaatcg ttccttcgtg gtgaacagcg ggtgcttacc cgggtttggg
      181 gcgatggcca caatgacccg cgagaacatc cggaatccgc gccggatgat atcactgtgc
      241 ccatgcgtga tcggatcgaa tgtgccggga tagacggcgg tcttcatgcc gggtgaactt
      301 ctttcggcgt aaggtgaaag cgggtcagtg cagtgtcgcc atagtcgtag cgcttcacct
      361 gcgaaagtgg tcccaggatg gagggcacgt ccgcttttcg tccatgttcc aggatgacga
      421 tggcgttctc cgccagccaa ccggcacgcc attctgccgc cagcgtaatc aattcaggtg
      481 tgagttgata gggtggatcg cagaagacca cgtcataagg acccgaccat tgcgtgccgc
      541 gacgaaaaaa ctgactcacc tgacaggcgc acacattggc ggattgttcc agcccgcatt
      601 gccgcagatt cgattggacc aggcgtagcg cctcgcgatc agcttccaca aaggtcacat
      661 gcgccgcccc gcggctcagc gcttcaatgc caattgagcc ggtccccgca tagagatcca
      721 atacgcgcgc ccccacgata cgttcgccaa ggatagaaaa gagggcttct ttgactcggt
      781 cggatgtcgg gcggatggcc tgtcccctgg gacccagcaa ccgtcggccc cgatgcagcc
      841 cagcgatgac gcgcatgatc gtattcaggt gagagccatt cgtctctggc taagaagtga
      901 gagaatgtaa cacagcgttt ttgaaggggt caaggtgact ggaggcggcg aggagcggcc
      961 gtgagccgga aaggtcttct ccccgccgcg gggcggggag cgctgtagac gtcaaggagg
     1021 agtgtacgcg ctttgacgcg caaaaggagg ctcgctataa tcgggcgaca ccaatactcg
     1081 gttcacgcgg gctgaggagg tggccaatcc tgggccgtca caccacccca tcacacagag
     1141 cgaacagagc gagggctccg cgtttagcgg gtcgccaccg gttgagcgtc gcgagcggcg
     1201 aggctctttc tacgattggc cgaaatggtt cggtcgatga tggctccgca gttgatgcat
     1261 ttccaggcgt agaaaatgag gaagaagtct gagaaccgtt ccaacatcat catacctttg
     1321 cacttcggac attccatggt ttcctcctgg gtgttgacgt ttacagctgt gtctgctctc
     1381 aagcaagagc tgcaccaaaa tgtaaaatct tttaatttca atatgttacg catcgaaagt
     1441 gccatatatg acatgcgcca ggcgtcaagt ctccggcaca aaatcgtgca cgctgacatt
     1501 tttttgtcgt aggaagccgc catgacacca aaaaagaccg gccgtggaat cggagattgg
     1561 ccaaaaactg aacggccgcg cgaacgattg ctcagtgagg gggctgaaag cctgtctgat
     1621 gcgcaattat tggcgattct gctgcgcgtg ggacggcagg acgcgtcggc cgtcaacgtc
     1681 ggcatggagg tgctggggcg cgtcggtggc attgtcgggc tgctgcactg cagcaccgaa
     1741 gaactctgcg cgattcccgg ggtagggccg gccaaggccg cgcaattgaa ggcagcggtg
     1801 gaggtaggca aacgagctgt ttcggcgcca ttgacgaccg gcacgcgaat caattccagc
     1861 gcggatctct ttaagcatta ccacgcacgc ttacgggacc tgcgtcacga gattttcgct
     1921 gtcgtattgc tggatgcgaa aaatcaggtc attcgcgacg tgacgatttc agaaggcagt
     1981 ctcacgctga gcatcgtgca cccgcgggaa gtcttcatcc ccgccatgcg tgcgtcagcc
     2041 gccggagtca tttttctcca taatcacccc agcggagacc ctactccaag ccaggaagac
     2101 cgtgtgctga cggccaggct cgtttcagcg ggggcgctgc tgggcattca ggtcctggat
     2161 caccttatcg tgggagacgg ccggtatgtc agtttcgccg accagggatg gcttggcgga
     2221 gaggcctcaa aagcatgacg gactgcctgt caccacggga cgaattcgta cggatgaaga
     2281 aaggatggtg tcgcccacag gtctctctct aaccaggccc cagcggccgc ccactaccgg
     2341 aggaggtcat gatgaatgtt cccctcaccg aatccatcag gaatgtggct gtggtatcga
     2401 atgcgggatc aggcaaaacc tcattagtcg aagcgctcgc ctgttgcgca ggcagccttt
     2461 ccgctcccgg gtctattcag gccggcacga ccgtctccga ttttgagccg gaagaaatcc
     2521 atcaccggac atccctccac accagcgtca ttcgttgcct ctacaacgac cacgtactca
     2581 atcttctcga caccccgggc tccccaagtt gcatggcgga actgcgagcg gcattgcgtg
     2641 ttgcggacgg cgtggtgctc gttgtgaacg cggcgacggg agttcgcagt gacctggaac
     2701 gcctctggtc gttcatccgt gaggccggcc ttccctgcat ggtgttcctg aatgatttgg
     2761 atcgggaagg gacgacattc gccacgacgc tcgaagaggt gacgaaagcg ctggacatgc
     2821 ctgctgtccc gttgatcctt gcgaatgggg agggcgggca attggggggc atcgtggatc
     2881 tgttgcagaa ctgtgtcgtg acggggcagg ccgatcgatt tcaagcgggg ccctctccga
     2941 ttccacccga atggcgcgat cggattgacc aggcccggcg cggcttaatc gagatggtcg
     3001 ctgaaggcga cgaggcgttg cttgaacggt acctgaacga tgggacattg acggacgctg
     3061 ccttgttgga gggattgcgg gtcggcgtcc agcgcggcgc gctggtcccc gtgttcgggg
     3121 gatcggcgtt gcgcaacgtg ggggtgcaga cattgttaca gggcctgctt caactcatgc
     3181 cgtctccggc tgcccgagct cagattgcac ccgtgcaggg agtcggactc tctgatggtg
     3241 agccgcccat gacgagggag ccggtcgcga ccgcccctct ctcgggactg gtattcaaga
     3301 ccatcatcga tccgtttgtc gggcgcctgt cgtacgtgcg gatctattca ggcacgttac
     3361 aggcggatag caatgtgtac aacgcgacga gacaggttcg ggagcgcggg gggcatgtgt
     3421 tttcgatctt cgggaaaaag tacacggctg tttcacgggc ggtggcgggc gatattgtgg
     3481 ccatcgggaa gctgaaagat agcttaacgg gtgacacgct gtgtgatgag cacgctccga
     3541 tccggctgcc cgggattcca cttggtcgcc cggtgatgtc gtttgccatc gagcccaaat
     3601 ccaaagctga tatcgaaaag gtgagtgtcg ggttgcacaa gttgatcgaa gaagacccca
     3661 gcttagagtt cgtccggcac atggaaacga aagaaatggt gctgagcgga atgggacaat
     3721 tgcatatcga tgtcgcgctg gaaaaactcc atcgcaagta tggcgccgac gtgacgctcc
     3781 atgcgccgaa aattccctat cgcgaaacga ttcgcagcgt ggcgcaggca cagggaaaat
     3841 ataagaaaca aaccggcgga catggacaat acggggactg ttgggtggaa gtcggtccgc
     3901 tcccgcgagg ccagggattt gagtttcaga acaaaatagt cggcggcgcc attccgcgta
     3961 attttgtgcc ggccgtcgag aagggggttg tggaagcact gcacgaaggc ccattggcgg
     4021 gatatcccgc cgtggatgtg cgggtgtccg tctacgacgg ctcataccat gtggtggatt
     4081 cctctgaaat gtcgttcaaa atcgccggat cgatggcggt gaaaaaagcc atggaagcgg
     4141 cccatccggt tctgcttgaa ccgctcatga ctgtagaggt cgacgttccg gcggagtgcg
     4201 tcggcgcggt gctgggtgat ctcaacggcc ggcgtggacg gattgtttcg gtggaagcca
     4261 gtggccatat agaaacggtg aaggctctcg taccccaggc agaaatggtc agctatgccg
     4321 cctcgttgaa ttcgctgacc ggcgggcagg gcagttatgt gatggaattt gcaagctatg
     4381 aagaggtgcc ccgggagctg gcgtcccgga ttgttgacga acacaaagcg gagcgcaatg
     4441 gggttgcggc ccattgacgg gccacgtccc cacctgacgc gatgcggcct tgttgaaatc
     4501 ggttagtgat cggctggttt cagcaccaca tagaacgcac gtccttcccg taagacgcgc
     4561 agaagaatcg tgtcgccgcg tttgaccttg gcaaccgcca cggtaaattc cccgaccgtg
     4621 ccggtgtcca cgcggttgac ctccttgatc aggtcgcctt cccgcaatcc ttctgcctgg
     4681 gcgaggcttc ctgcgtccac ctttgaaatc agcacgccgc gactttcatt caatcgaaac
     4741 ttttccgcca ggccggccgt caagtcctgc acatcgatgc ccaattttaa atcggtccgg
     4801 ctctgcggca aggaggcggt cacgaccgta tccttgcgtt cgctcagggc gacctggaga
     4861 ttgaggcgtt tcccgtcgcg tacgacttcg acacgcgtcg ttgacccggg gtccaatgcg
     4921 gcaacgaggc gagacagttt gttcggcgta tccacgagcg ccccatcgac cttcgtgatg
     4981 acgtctccag gcttgatgcc ggccaacgcc gccggatccc gctcgaacac ttcattgacc
     5041 agaacccctt cgttctcatc caccccgaac ttgctggcca gttccggcgt cagcggttgt
     5101 aacccgaccc ccaaccaggc tcgcacgact ttgcctttcg cgatcagctg gttcatgacc
     5161 tgcttggcca tgttggacgg gatggcgaaa ccgatgccct gcgcaaaatt gataatggcg
     5221 gtattgatgc cgatgatatc cccacggaga tcaaacagcg ggccgccgga gttgcctgga
     5281 ttgatcgacg cgtcagtctg aatgaaattt tcgtagcggg agagattgat gttctcacgt
     5341 ccgatcccgc tgaccacccc cagcgttacg gtgcgatcca ggccgaacgg attcccgacc
     5401 gccagcaccc actggccaac cttcacgccg gacgagtcac cgaaatgggc cgcgggcagg
     5461 ggatggtcgg cggtgacttt gagtacggcc aggtcggtgt ccggatcctt accgatcacc
     5521 tgcgcaaaca acttcgtttt gtctgagagg cgaacctcga cttccgccgc gtcgcccacc
     5581 acatgattat tggtaatgat gtggccggcg gcatcaacga tgacgcctga ccccgatccg
     5641 gtcgagctgg gaacccgctc gcctccgtct cgagatcggc cggtggcctg gatcggaaag
     5701 atgctgacga ccgatggctt ggcctgttcg gcgaggtcag tgatgacggt ctgcagctct
     5761 tccaacaggc gtaatcctgg cgacacggtg cgtggcgtgg aggcgacgtc ggctgcagcc
     5821 acggcggaca aagggcttcc caggaacgat ccagtcagaa taagtccaag acacagcagg
     5881 gtagaacatg acagcggtgt tttcatgttg aggcaagctc cgagggcgat accgaagcac
     5941 tctggcgacg cctcagaata ccagattttc gcgtgacggc gaaaggaagg acgggtcagg
     6001 ctgatttgga tgcggccggg tgctggaagt gcgggagcca gtgggccaag tcgtggcgaa
     6061 ggccgacaag gatgacgatg tcatcgggac gaagcgtcat ggtgtgggag agaggcagaa
     6121 gaaacggccc gcgaaagacg gcggcgatgc gggtcgtggg cgggaggtcg aattcgccga
     6181 tcggatggcc gatgaccggc gcgcctcggg cgaccaccac gcgttcaatg gcggcgtcgc
     6241 ggagacccaa ttcgcgttgc atcgtcaaca ggtcttcgtg agccagctcg accttgagct
     6301 cacggatctg gcgttccaca agttgcaacg cggcttgttg gcccttgatg cggtcggagg
     6361 cggtccggat tgcgtcctgc agcgaggcca attcggaagg cgctaccgga tggattcccg
     6421 cagccggttt ggctgcgaga gcggcaacgc gttgtccgat ctggcgatga atggcgtgcg
     6481 tctggttcag gatcgtcgtg gcctggccat ggaggcgcaa gacatggact ttgcggttga
     6541 cgcgttccgc gatggcgaca atcgtctcgt agagggcgct gccggtgagg aacagctctt
     6601 tgcggatgcg agccaatagc tcaaaagaca gcataacggt aatgtctgat aagatatatt
     6661 atgtcaactc gattgtccga tttgagattc cgtcccgctc cacaatcgga tcaaattgtc
     6721 cttgtgcctc atccagatca gcacactgac gacacaggca aatccgataa acgtccagga
     6781 gcgatggaag atcagcgcca caatcgggaa catgccaaag gcaaacagcg cgcctccgga
     6841 agaatacttc cacaagaaca cggcgccgat ccagagcgtc atgagtgtga gcccgaccca
     6901 aggggcgacg cccagtgccg cccctaacgc tgtggcgacg cctttgccgc ctttcaatcc
     6961 aaggaatatc gagtgcaaat ggccgagaat tgacgccagg gcgaccccta aaatggccgt
     7021 ttcctgctgc agcaccatcg tggccaccca ccctgccagc cagcctttgc cgatgtcccc
     7081 cgccagcgtc aggacaccgg cttttttgcc gctcacgcgc agaacattgg tgaagccgac
     7141 attcttgctg ccggcggttc gcggatcgac ggcgcccagc aggcgcgaca cgacgacgcc
     7201 aaacggaacc gatccaagga gataggcgaa gatgatgaga acaccgatga gccagggact
     7261 gtccatcacc gagtgcgtgc cgccgggttg ttcacctaca gaccttttgc tcccacctgc
     7321 cgccagaagc tccagacata ctgtgcgccc gacacatatc ccaggatcaa cgagaggtag
     7381 agggtgacga tgccggcgag atgcagattg ccgatggttt ccacgacggt gccttccagc
     7441 accagaagga cgatggcgat gacctgcaac gccattttgt atttgcctgt gacctcggca
     7501 gacatgatca atccttcgga ggccgcgatc gcgcgcaagc cggtcacggc cacctcgcgc
     7561 gcgataatca ggatggctac cagtgcgctg acgcgatcga cttgcaccag cagaatcaag
     7621 gcagacaaca ccagcagctt gtcggcaatg ggatccagaa ggcgccccag cttggtgatc
     7681 tggccggttt ttctggccac gtagccatcc agcagatccg tcacggccgc cacgacgaac
     7741 acaatggcgg cgctcaaggc gcggtctggc gtcggatcga tcagcaacat gacgaacacg
     7801 ggaatcaaca ggatgcggac gagcgtcagc acgttgggca ggttgatgtt actttcctgc
     7861 cctgccgacc gcatcaggac cagtcctgct tctttccagt tctctcccat ggtctgtgtc
     7921 gcccgaagtc tccgatgttc gcgaaggttc ctttatacaa tgccgtgttt cagcaggtca
     7981 tgcaagtgaa tgaccccggc caggcgattc ggtggctcga tgaccacgag tgccgtgatg
     8041 gagaaacgct ccatctgtgc gacggccgtc gtagccaatt catccggacc gatcgtcttc
     8101 ggatggcggc tggccaggtc accggccgtg acccggctga aatcgccgcc ggcttgaatg
     8161 aagcgccgca agtctccatc ggtcacaatg ccgtatagcg atcccttggc atcgaccacc
     8221 gtcgtcagcc cgagtttctt cgaggtcatc tccaggatcg tgtccgcccc ggacacgtcg
     8281 gcgcgcacgc gtggcaaatg atccccatgt tgcatgagat cacgcacctt gacgagcaaa
     8341 cgacgcccca ggctgccgcc gggatgaaat tgtgcgaagt cgtcatgttt gaacccgcgt
     8401 ttttgcagta aggccacagc cagggcatca cccatcgcga gcgtggcggt cgtgctggcg
     8461 gtcggagcca ggcccagggg gcaggcttcc tcatcaacgg acacatccaa cgtgacatcg
     8521 ctgtttttcg ataacgtgga ggtcatcttc cccgtcatgc cgacgacagg cacattcatt
     8581 cgtttgacga acggtagcag ttgaaggact tcctgcgtct ccccgctgtt ggagatggcg
     8641 atgagcacat cacgccgtgc gagcatgccc agatcgccgt ggactccttc ggccgggtgc
     8701 agaaagaagg acggtgtccc ggtactggcc aacgtggctg caattttttg tccgatgagc
     8761 cctgattttc ccatgccgga aatgacgact ttcccctggc actcgtacaa cagcgtcacg
     8821 gctctggcga atcgctcatc cagacgggcc ttgaggtcga gcactgcgcg cgcttcgatg
     8881 tcgagcacgc gaatgccctc gcgcacgctc gcatcgacgg cggcagaacg cccggcggat
     8941 gtcttggtct tgcgcgttac cggactggtt gtggagcggc tgcgtcgcat atacgtaata
     9001 cccgttcaag gagtgaatga agcgcatgaa gcggaaccat gttcggtcca tcggatagag
     9061 cggaatcggg atccgggtgc acctccatga agaacccgtc gcagccggct cctgccgcgg
     9121 cgcaggccaa gggttcaacg aattcacgct ggccgctgga tttggtgccg cccccgccag
     9181 gcagctgtac actatgagtg gcatcaaaga ccaccggata gccaaagcgg cgcatgatgg
     9241 gaaatgagcg catgtcgacg accagattgt tgtacccgaa cgaggagccc cgctccgtca
     9301 ggacgatacg gcggttgccg gcgtcttcga ccttcttgac ggcgttgccc atttccggcg
     9361 gcgacaggaa ctgccccttc ttgatgttca cgaccttgcc ggtcttcgcg gcggcaatca
     9421 acaggtcggt ctggcggcag agaaaagcgg gaatctgcag catgtctacg acatggccgg
     9481 cctctgtcgc ctgctcttcc gaatggacgt cggtaagaat cggcacgccg atctgctgct
     9541 tgaccttggc cagaacggca agaccctttt ctaagccggg tccccgaaag gaggagatgg
     9601 aggtgcgatt cgccttatca aaggaggatt tgaacacgta gggcatgccg agtgatttgg
     9661 tgatttcggc gatacgtccc gccgtatcca tgacgagttg ctcgctttca atcacgcaag
     9721 gccccgcgat caaaaacggc cgactccccg ctccgatctt aaattgtccg agatcaactt
     9781 cgtacgccat agtcttctct tctgaattcg cgggatgctc aaactgatct ttcagcaagg
     9841 ccgcagacga ggcagagccg gaggcgtagc ggagctacgt cgaggactgt gccgagtcga
     9901 gaacgccgca gaaaggcagt ttcagcatcc catttagtgg ccgcatttgc ggcgtagtgc
     9961 cgcccccaca aatccgctaa acaaaggatg cggatgatgc ggccgtgact tgtattccgg
    10021 atggaactgc gtggccagga accagggatg attttgcagt tcgacaattt cgaccaggcg
    10081 gccatccggt gacaggccgc tgagaatcag tcccttcgcc gtcaattgtt cgcgataggc
    10141 attgttgaat tcataccggt ggcgatgccg ttcgcgcact tcgctgacgc cgtacatttt
    10201 ttgcgccagg gtgccttctc ccaatgtgca ggcgtaggcg ccgaggcgca tcgtgccacc
    10261 cttgtcattg acggattgct ggtcagacat gagatggatg accggatgcg gcgactgcgc
    10321 atcaaactcc gcgctgttcg cgccggtcaa tccagcgacg ttgcgcgcaa actcgatcgt
    10381 ggcgcattgc atccccagac agaggccgag gaagggaatc tggtgttccc gcgcatactg
    10441 aatggtgaca atcttgcctt cgattccccg tgagccgaaa ccgccgggaa tgagaatgcc
    10501 atcggcctcg cgcaagattc gttctgtccc ctggcgttcc acgtcttcgg attcgatcca
    10561 cgtgacgttc acgcgggttt cgtggtcgat cccgccgtgc accagggcct cagccaggct
    10621 cttgtaacat tccttcagtc cggcatattt tccgacgagc gcgatcgaca cttcgtgttt
    10681 gggatgcttg atcttctgca ccatcgcgtc ccattcgcgc agattgggcg ggccggtttc
    10741 gagcttcagc tgccggacga tcagctcatc cagcccttcc ttgcggaaca cgatcggcac
    10801 ttcgtagatg gtttccacat ccttggccgt aatgaccgcg tccttttcca cgttgcagaa
    10861 catggcgatc ttggctttga gctcgggtgg aaggtagcga tccgtgcggc ataacaggat
    10921 gttcggttga ataccgattt cgcgcagctt gttgacggaa tgctgggtcg gtttggtttt
    10981 caactcacct gcggcgccga tgtacggaac cagggtcagg tgcacataga gcacgttgtc
    11041 gcgtcccacg tcatagggaa gttgccgaat ggcttccaga aacggcaggc tttcgatatc
    11101 gccgaccgtg ccgccgattt ccacgatcgt gacgtccata ccttgcgacg tccgcatgat
    11161 gcattgcttg atctcatcgg tcacatgggg aaccacctgg accgttccgc ccaaatagtc
    11221 accgcgccgc tcttttgtga tgactgagtg ataaatacgg ccggtggtgt agttgttttc
    11281 gcgcgtcagc gtgagcgagg tgtatcgttc gtaatgcccc aggtcgagat cggtctcggc
    11341 cccgtcttcc gtcacgtaaa cctcgccgtg ctggtaggga ttcatggtgc ccgggtcgac
    11401 gttgatgtag gggtcgagtt tcaaaaaggt gattttcaac ccgcggcttt ccaggagatt
    11461 gccgatggac gcggaagcca gtccctttcc gagcgacgat acgacacccc cggtcacaaa
    11521 gatcagcttg ctcatcgtgg caccttcttg tgaatgcggc caatagtcat ggttgagtgc
    11581 ttcccgctct gctcgtatga gcccgaagtt gggtcaaggc gaccggcacg tcctcggggc
    11641 tatcgatgcg taaggacatc tgggaggttt cccagacgcg aatcctgatt ccgttctcca
    11701 gcgcgcgcag ctgctcgagt ttctccgcgt cttccaggat cccggtcggt aatgccgtca
    11761 ggctcgcgag ggtctcgcgt gtatagatat acagacccaa atgaatgaaa tgtaatcctg
    11821 ggacggccga ccgtttcgga tcgtcacgaa ccagcgggat cggtgcccgg gaaaagtaga
    11881 gagcattgcc ttcccggtct gtcgtgactt tcaccacacc ggtattctgc aagtcctggt
    11941 cggagtcgat ccgccgtttg agtgtgccca tcggcgcgtc gctggccagg aatggttcga
    12001 ccaggtccgt gatcaggtcg ggatgcagca gaatttcatc cccctgcagg tcgatgtagc
    12061 aatcgccgcc acgagacttg gcaacggctg ccacgcgatc ggtgccggtg cgatagggct
    12121 ccgtcatcat gaggacctgg cctccgaatc cctcgacggc cctcttgata cggtcgtcgt
    12181 ctgtggcgac cagcacgtca tccaccacgc ggcaggcctt tgcctgctca tagacatgtt
    12241 ggatcatcgg cttgcccatc aagtcgatca acggtttccc cggaaaccgc gaggatccgt
    12301 accgggctgg aatcaccacc gtaaccgaac gtttcacctc agctctgtcc cagggcatcc
    12361 gccaattggg cggcggtcac cgtggcggtg ccgaccatgc ccaccacaac cccggcagct
    12421 tgattcgcga ggatggccgc ctcccgcatg ctggctccgg tcgagagcgc cagcgccagc
    12481 gttccgacca ccgtgtcgcc ggcaccggtc acatcataga cctgcctggc gcgggtggga
    12541 atatgccaat ggtcgccgtg cccctggtag acactcatcc ctcgctcgcc tctggtgacc
    12601 agaacagtct ggcatcccaa acgttggcga aggaccatcc cggctttatt gatggtcttg
    12661 tcatcttcgc cttgcacgcc ggccgcctgt gtggcttcca aatggttggg ggtcacgacg
    12721 gtcacgccct tgtaataact gaaatgctcc accttgggat cgacgacaat ggggatcttg
    12781 cgctgtcccg ccaaacgagt caactctgtc atcagcgagg ccgtgacgac gcccttggca
    12841 tagtcggaga ccaccagaca ggagagttcc ttcaggcgtg attccacgta ccggagaata
    12901 cgccgctgca ggaggctgga gagttcgttg cggcgttcga cgtcatagcg aacgatttgc
    12961 tgattgtggg cgacgacgcg tgattttctg gtcgtcggcc ggctttgatc gatgacgact
    13021 ccgccccgtc cctgcctgcg cccgcccaat tctttcagga ggagccgtcc gctttcgtcc
    13081 gccccgatga ctccgcagat atccgcctgg ccaccgagtg ccagaatgtt gttgaagacg
    13141 ttcgccgcgc cgccgagctt cagtgattcc gattcgacat gcaccaccgg caccggagct
    13201 tccggagaga tccggctgac ccggccccag acgtagtgat ccaggatcaa atccccgatg
    13261 accagcacac tggcccggga aaatcgctgg atgtacgcgc cgagtccagc gtgatccatc
    13321 gccgcctctt ctgccgcgtg cccgttcgac ggtccggcat ggccggatcg ccctactgga
    13381 tggcttttcc taacctttcc catcgtaccc attccgtcca cgatccttgg ccgctccacg
    13441 accagtaaat gcgaaatgct ttgccgcgca ctttctccgt gcgcacgtat ccccagaacc
    13501 gactgtcgag actgtgatcc cggttgtccc ccatcacgaa gtaggcatcc tccggcaccg
    13561 tcaccgggcc gaaattgtct cgcggactga tgtgcccgtc gtgaatgggc ggatcggtgt
    13621 gctgcgtgaa ggcatggtca tcgaacgggg ttccgttgat gtgcaccacc ttgttgcgta
    13681 cgtggatggt gtcacccggc agcccgatca cacgcttgat gaagtccttg tcttcgtcct
    13741 cggggaaccg aaacacgatg atgtcgccgc gttgaatcga tccgaacgga atgaccgtgt
    13801 gcgaggaata gcaggtgact ggtggaaagc ccggttgaaa cttgcagtcc gtcggccact
    13861 ggagaccgta ggataatttg ctgacgagga tatggtctcc caccagaagc gtgggaatca
    13921 tcgagccgga tggaattttg aaggcctgca ccacgaagac acggatggcg aacgcgagca
    13981 acatggcaat gatgatggct tccgcatatt cgcgcaggat cgacttgtgg gccggctggt
    14041 tggccacgac caggcgtggt tcggtggtcg ccggttcggg aggcgtgggt gggggcgccg
    14101 gtgcattcgt cgcagggatt tcctccggac gggcattggg gtccacgctc attcctcgcc
    14161 caccttgaga atggccagga acgcttcctg cggcacttcc acgcgtccca ccgctttcat
    14221 gcgcttcttg ccttccttct gcttgtccca cagcttgcgc ttgcgggaaa tgtcgccgcc
    14281 gtagcatttg gccgtgacat ttttcttgat ggcgccgatg gtttcacgcg caatgatctt
    14341 gttgccgatg gccgcttgaa tggcaatttc aaacatctgt ttgggaatga gctctttcat
    14401 tttttcggcg acctggcgac cgcgctgata ggcccgttcc ttatgggtaa tgaacgagag
    14461 cgcgtcgacc ggctcgccgt tgagcagaat gtccaagcgc accagttccg actcgcggta
    14521 gccgagcagc tcataatcga gcgacgcata accctgggtt ttcgacttga gcttgtcgta
    14581 aaaatcgaga atgacttcgt tgagcggcaa ttcataggcg atgaccacgc gggtcggatc
    14641 cagatagtga atgctccgtt gaatgccgcg gcgttcctgg cacaattgca gcaacgtgcc
    14701 gaggtaccgt tccggcgcga tcagcgtggc cagaataaac ggttcttcga aggaggcgat
    14761 cgaactcggt tcaggcaatt ccgcagggtt gttcagttcg aggacatccc ctttggtggt
    14821 gaggatccga tagatgacgg tcggtgcggt ggtgatcagg gtcagcccgt attcccgctc
    14881 cagtcgctcc tgaatgattt ccatatggag caggcccagg aatccgcagc ggaatccgaa
    14941 tcccagcgcc agcgaggttt ccggctcgta aatgaaggac gagtcgttca ggcgcaactt
    15001 taaaagcgca tcacgcaaat cttcgtagcg cgcggtgtcc gtcgaataga ggccgcaaaa
    15061 aaccagcggt ttgacttcct tgtacccggg gaacggctga tcggtcggcc gtgccgcatc
    15121 ggttaaggtg tccccgatct tcacgtcggc tacttccttc atgttggcgc agagatatcc
    15181 gacctctccc gtcaggagtt gcgtgccctt cgttcgcttg ggggtgaact gcccgacttc
    15241 cgtcacttcg aacaaccggt cgttcgacat gaccttgatc ttcatgcccg ggcgaacgga
    15301 cccgtcgatg atgcgggtca ggacgatcac gccttggtaa ttgtcgaacc aggaatcgaa
    15361 aatcagcgcc ttgagcggac gagtcggatc accggacggg ggtggaatgc gtttgacgat
    15421 cgcctcgagg acctccggaa cgccgagccc ttcctttgcg ctcacgagca tggagtccgt
    15481 ggcatccaat gtcagcacgt cggagatctg ttgtttggta ccttcgacgt cggcgctggc
    15541 aagatcgatc ttgttgatga ccggaatgat ggtgtgatgg ttgcccatcg ccaggttcac
    15601 gttggcaatg gtctgggcct ggacgccttg tgtcgcatcg acgagcagca gcgatccctc
    15661 gcaggccgca agactgcgtg atacttcgta cgtgaagtcg acgtgccccg gcgtatcaat
    15721 caaatgcagt aaataggtct tcccgtcctg ggccttgtag cggatggcta cggcgtgagc
    15781 tttgatggta atgccacgct cacgctccaa gtccatcgca tcaaggatct gctctttcgc
    15841 ttcccgggca gtgactgcgc cagttgcgtc gaggagccgg tcggcgaggg tcgatttacc
    15901 gtgatcgata tgggcgatga tcgagaaatt tcggatgaga ctttgcaaat cctggctcat
    15961 attccccaaa atcggttcat tatagtgacc gcctccaacg ttgtcaaaga aatcgcccgc
    16021 cgttataatc cgcgatcggc gcggcgtttc atgcatgggc gcatctacga tcgcgcactc
    16081 gcaacatcac accgcagtac cagatgacac ctccatccac acgccaaccg cttgccgcct
    16141 gggatcatgc gtatgtgtgg catccgttca cgcagatgca ggaatgggaa caggagagcc
    16201 ccctcatcat cgaaaagggc aaagggtcct atctcatcga tacggaagga cggaagtatc
    16261 tcgatgggac cgcttccatt tgggtgaatc ttcacgggca tcggcatccc cggctggatc
    16321 gtgccctcac cgcgcagctc aggcagatcg cgcattcgac ctttctcggg ctcacgaatc
    16381 cgccggcgat tcgtctggct cgcgaactca ttcgccttgc cccgaagggg ttgcggcggg
    16441 tgttctattc cgacaacggc tcgacggcgg tcgaggtggc gctcaagatg gcagtgcaat
    16501 actggcagca agcccggccg tccaacggat caaaacagtc ctttgcgcat ttgaaaatgg
    16561 cctaccacgg cgacacgatc ggcgcggtca gtgtgggcaa cattgagctg ttccatgggc
    16621 gtttcaagcc gcttcttttt cccacgcatc aggtcgaacc gccctactgt tatcgttgtc
    16681 cgctgggtcg ggcgtatcca tcgtgcgaca tggcgtgcat cgatccgctc gaacgcctac
    16741 tcaagacgcg gcatcgcgag ctggctggtg tcattgttga gccgctggtg caggccgcag
    16801 ccggaatgat ggtggccccc ccgggatatc ttgcccgcgt acgcgagctc tgcacgaaat
    16861 acaatgtcct gctgatcgtc gacgaagtgg caaccggttt cggacggacc ggcaagatgt
    16921 tcgcctgcca gcatgagggg gtcacgccgg atctgatggc aatcagcaag gggttgaccg
    16981 gcggatatat gccgttggct gcgacgctga caacggacgc catctatcgg gccttcctgg
    17041 gacggtatga cgagtggaag acgttcttcc acgggcacag ctatacggga aatcctctgg
    17101 gctgcgctct ggccctcgcc aatctggaga tcttccgccg cgaacgcacg ctcgcccagg
    17161 tgcggaagaa atcgcgcctg ttggcccgtc tcttacaacc cctggctgag ttgccgcatg
    17221 tgggtgatat tcgtcagtgc ggtttcatgg tcggcatcga gttagtgcag gatcgtcaaa
    17281 cgaagacccc gtatccgctc gaatggcgca tcggccatcg agtggcgcag gcctgccggc
    17341 tccgtggact gcttctgcgg ccgctcggca atgtcatggc gctcgtccct cccctctcca
    17401 tctcgcctcg tgagctcact accatgatcg caattttgca cagtgcgatt cgtgagatca
    17461 cagaggaact cgctacctcg cgctgaaacc gcgcgtcctt cctgctgatt ggtgctctcc
    17521 tgcagtcacc gttcggtgtt cacgccggta cagtctctcg acaggttttg tgcactgcca
    17581 gggcggccgg aagcgtgaaa gtcgagtgct acacttgatt tcatgctgtt ctacgcagtc
    17641 acctcggcga gcacggtttc atgacgacgg gcctgatcat ccgggtcctg tgcctgctcc
    17701 ttttcctgcc cttctcactc tgggccgaag accagcaacc gtcggaactc ctccttcagc
    17761 gcattcagtt cagcgacacg gctcgcgagt ccgggactgc cgcgattccc ttttccattg
    17821 cctcaccggt catgcatacg tcgtttcaag caacgtcctt tgcgccgccg acgacctcca
    17881 ccagactgaa agacctggtg cctgattccg aacggcctca aaccaaagga ctcggcgcca
    17941 attccacctg gctcaagggg cagctcacgg cagaaggcga agtggccaac aatgcgacca
    18001 acgatgcagg cctcacctcc cgcatcgatc aacgtgatga cggcgccaaa cgcatggtgc
    18061 gcatggcgct gaccggcacc aacgggccgg tccgatacgg catcaactat cgatcggccg
    18121 ggaaagcctt tttcaactcg ccggatcaga cggtgcggga aatgtgggca gaatgggggc
    18181 tgggcattgc gaagctgcgc agttcctccg ggcagacctg gaacaatgtg gatctggagc
    18241 cgaaccggcc gcgcattcaa caggacgtga gccgtatcgg catcgcgctt gtgaaaccgg
    18301 cgtggcctga gctgagcctg acctatgcgc gaagcgccct ggcgagcagc tatattcctg
    18361 caggcatgac cgctcagcgc agccagagta acagcatgga agcggccctc gcctataccg
    18421 gcctttcctg gaatgccaga ctctcgtcga gttacatcct gacgaacgat gagatgcggg
    18481 ggggagccca gtccacgacc tttgcccaga ccctggttgc gtcctatcgt cccctcaata
    18541 ccctgacgtt cgcgccgaca ttcagttacc gcacggaaac gcagagctgg tcgggcgcca
    18601 agatccagac gccgatggcg tccgtgtctc tgctgtacaa acaaagccag cgattactgg
    18661 tgagcgcgat gggcggctac accagtacaa agtcgagcga cggattgatc gaaacggaga
    18721 gtatcgtggg ccgggggatg tttgcgttcc atctcaaccc gatttacggg catcccacca
    18781 ccctgtcact ggaagcctcg tataccaata cgacgaatcg gacggtggca ggagtcgata
    18841 ccgaggattt gtccgggttg gtacggattc ttgtcgcggc gttatagccg ctgccgatct
    18901 tcgccgtgag ccggtctagg ccccgccagc agaagtatcg ctcgcagcca ggtggttgat
    18961 ggacgagggg ctgagaacaa tgcgttctcc ggtcacgagt ccttctcgaa tcgtcacctg
    19021 attgtctgtc acgctatcga tggtcacggg acgttcctca aaccggcctg gtgcgagctc
    19081 cactacgatc cattgcctgc cggatcgttc catcactgcc tgcttaggaa tcacaatctt
    19141 cgggcgcatg gtgtggccgg caatcgtcag cctggcgaac atttccggtt tcaatcggcg
    19201 atcctgattg gaaaccgtgg cgcggatttt gatcgttctg gtcgccgggt cgacgacatc
    19261 gccgatgacg gcaatctttg cgggaaattc gatggctgga taggcttcta ccgtcatggc
    19321 cgcgagggcg ccgactctga tgccggagag gtcgtgttca tacacatccg cgaccacctg
    19381 cagtcgatcc agattggcca cggtgaacag cggctggtcc gaagctgttc ccacgatttg
    19441 ccccggcgtc acattgcgtt caacgacggt gcctgtcaac ggactgcgga gctcgaagcg
    19501 cgacgtgatt tgctgttgtg cgaggggttt gctgagttcc gcggccggaa cgcgcaggga
    19561 aaggagcagc tccttcgcct gcttgaattc ggcccgctcg cgattcagat cgttttccgc
    19621 atgctccaga tccttcaagg acatggcctg ggcgtcgtag agatccttcg tcagttcata
    19681 gttccgttcg gccaggccca attccgaaat ttcctccaca taggccgcgt aggcgcgagc
    19741 gatatccgcc gcgtcgatca ccatcaggat gtcgccggcc ttgaccgcct ggccgagctt
    19801 cacccgtacg tcgagcaccg gacctggaag tggggaggaa atttttgagt atccgtcttc
    19861 tgcgtacgtc actctggccg agagggtcag cgctggcaga gatggcgagg catcaatgcg
    19921 cattgtctgg atgtctggtt gaccgttggc gacgactgat ggctgtacag gtttggcgga
    19981 tggcgcggga ggatcagggc gtgtgcagcc catgacggtg agacagagca gcacgaccga
    20041 aagatgttgc agcaatggtt tcatggaacc ggtgtcctct tatctcatat cggtcgatgt
    20101 gtcattcggc catagtcctg cccctgagcc ggctcgaaac ggtcgccccc ggcagggact
    20161 cgtctggagg gatcacatca gtgtacagga gcagcgaagg cgggacgcag gaccaacccg
    20221 gaatggtgag ctcccccggc tggatgaaat atgcagaaag atcagcctat tccgtgatgg
    20281 ccacgtcatg catcccggcc cgcaagctca ccatctcatt aaaacaccgg caactttcgc
    20341 ctgtcaatta cgggagattg catgaggcac ggaagcagtc tggccgggat cgcgcacggc
    20401 attgtttaac tgagctcaat gcgcgcgcct ctggccacat tcaaccggtc ggccgcgcga
    20461 ccttccttga ggaacacctc gacgtacccg ttgctgttga tcagagcctg gggagcatcg
    20521 gctgacccct ctccgtaact atgaaccagg cagtcgatcg tgaccccgcc gatgcggatg
    20581 tacggatcgg cgcgtttggt gatcccacgg acttcccgga tatggtacgc ggtgaggttt
    20641 gaaatcagat tgccgaaacg atcgatatag gcgatctccc cggccattac atgtttgtcc
    20701 caggacggtt cggaaatcgg cagacgttcg taattgggga cgagccggcc gaacgacccg
    20761 atcggttgtc ctttggtcaa ccaggctgcc gccggggcga agagatcacg cccgtcgaag
    20821 gtggcgcctt cggaatcgag ccggtattgc cggttttcga ggtgccggac ttcaacgccc
    20881 gtttcttcct gatagatgtg ggtgaacagg ccgttgtccg ggccgataaa aaagtagcga
    20941 gaggacgaga ccagcagggg gcgcctggtt gtgccgacgc ccggatcgac gacggcgacg
    21001 tggatggttc cgtcggggaa ataacgatag caggatttga gcagataggc ggcatcggct
    21061 acatcgtgcg gtgtgacatg atgcgaaaga tccacgatgt gcgcttgggg attgatgttg
    21121 aggatgacgc ctttcacgct cgccacgaaa taatcgcggt cgccgaaatc cgtcagcagc
    21181 gtgatgaggg gaatggttac cggcatgaga cccaccgggc ctgcgacggt gaacggaacg
    21241 gcgcgaggga ggaacgcgtg aatgacgtga ggggcgacat cacggctata attcctcgaa
    21301 gcggatttcc atcacttcgt attccaccac tttggcagga gtcgtcacct cgaccaaatc
    21361 cccgacccgc ttgccgatga gggcgcggcc gaccggcgat tgcactgaaa tgcgggaaaa
    21421 tttgagatcc gcttcatcct gtccgacgag ggtatactgt ttcttggcct gcgcttcctg
    21481 ctcgatcaac acgacggtgg cgccgaagac gacggtgtcg ctggtgcggc cggcaatgtc
    21541 gatgatccgg cagtccgcca gcttgccctt caactctgcc aggcgtgctt cgatgaagcc
    21601 ctgtcgttcc ttcgcggcat cgtattcggc attctcgctc agatcgccat gcgcgcgcgc
    21661 ttcggcgata gcctcgatga cgcgcggccg ttccacttta tgcagccggt ccaattcggc
    21721 tttcagcgct tcgtatcctt ttctcgtgat cggtgtcggc atatcttccc cccgatgacc
    21781 attgaaaccg gcttccggtt ggacctttcg gtatcgtccg gttgcccgca tccaggaacc
    21841 gtgcggcgga ctcatgtccg ttcctggaac ctgttgtcaa ttgtccctct gtcaggacct
    21901 tcgctcaatt cacccggtga tactcctgca atgctcgaat ggcaagcccc tttttcagca
    21961 acgcttcgat ccccatcacc gctgccaggg ctccgcgcat cgtggtgtag tacggcacgc
    22021 ctttgtgtag cgcctcacga cgaatagaca gcgagtcgga atgtccggac gcggtgcgta
    22081 cggtgttgac gaccaaggcc acgtctccgt tcttgatatg gtcgacgatg tgcggccgcc
    22141 cttcctgcac cttgttgacg acgtcgacct gcatcccctg ctcggtcaga tacgcagccg
    22201 tgccggaggt cgccgtgata cggaaaccca gcgcgaccag ccgttgggcg acatcgcagg
    22261 ccacagcgcg gtcttcactt ttgacactga gaaaggcggt gcctgaagtc ggcagaatgg
    22321 cacccgcgcc ggcttgggat ttgacgaaag cccagccgaa atcgctatcg atgcccatga
    22381 cttccccggt ggacttcatt tccggtccga gcagcacgtc gacccccgca aatttggtga
    22441 aggggaagac ggcttccttg accgagagat gcgtcggcat cggcgctgtg gtgaagttca
    22501 attgctggag cgacttgccg accatgactt tcatcgcgag tttggccagg gggacgccga
    22561 tagccttgct gacgaagggc acggtgcgcg agccgcgcgg attgacctcc aagacgtaga
    22621 tggtctgatc ttttacggcg aactgcgcat tcatgagtcc gatgacgccg agttccaaag
    22681 ccagcgcggt catttgccgg cggatttcct caatcgtcgc ggaatccagt gtataggggg
    22741 gcaacgaaca ggcggagtcg cctgagtgca cacccgcttc ctcgatatgt tccatgatgc
    22801 cggccacgac gaccgtcgtg ccgtcggaaa tcgcgtcggc atcgatttcg atggcatcgc
    22861 gcaaatactt gtcgatcagt accggatgct gtgccgaggc tttgactgcg gaattcatat
    22921 agtgcaagag gcccgcctcg tcgtagacga tctgcatcga tcgcccgccc agaacatagg
    22981 acggccgcac cattacggga taagtaatct cagccgcgat tttcagtgct tcgtcgacag
    23041 agtgggccat gccgctctcc gcctgacgca agccgagttt gtcgagcagt tcgcggaaac
    23101 gtgcacgatc ctccgcgcgg tcgatggcat ccgggctggt gccgagaatc gtgacgccgg
    23161 cgcgcgacag ggagagcgcc agtttcagcg gtgtttgtcc gccgaattgg agcaccacgc
    23221 ccatgggctg ttcccgttcc acgatattga ggacatcttc ttccgtgagc ggttcgaagt
    23281 agagccggtc tgaggtgtca tagtcggtgc tgaccgtttc cggattgcaa ttgaccatga
    23341 tggtttcgac ctgttcttcg cgcaacgcca tagcggcatg cacgcagcag tagtcgaatt
    23401 cgatgccctg cccgatccgg tttggtccgc cccccagaat caccaccttc ttcctggtgg
    23461 tcggccgcgc ctcgcattcg cgctcgtagg tggaatagag gtacggcgtg tgggcttcaa
    23521 attcagcggc gcaggtatcg actcgcttgt atgtcacgct gcgaggagtg ttcccctggc
    23581 ctagggccag gcgccaccgt cgaaccgtac tttgtggaac ccccagcaat tgcgccaggc
    23641 gttcgtcagc gaagcccaac tgtttcgctt cgaccaacag ttccgggcga agtcctgcgt
    23701 cgccgagtgc gccacgttcc gccacgattc gcggttcgaa ttcgacaatt tcacgaatct
    23761 gttcgagaaa ccagggatcg attttggtga gcgcaaagag ttcctgattg gtcataccaa
    23821 gacgcatgcc gtcggccagg cgccagagcc ggtccgggag cggtgtgcgc acggccttgc
    23881 ggacctgttc cgccgcttcc tcgcgattga gcgacggggg caccccgagg tcgaggccca
    23941 tcttcgagct gaatccgaac tgatcgactt ccatcgaacg gattgccttc tgaagcgatt
    24001 ctttgaacgt gcgtccgatg gccatgactt cgccgaccga tttcatctgg gtggtcaacg
    24061 tgggatcggc gccgggaaat ttctggaagg caaagcgggg aattttcaca acgacgtaat
    24121 cgatcgtcgg ttcgaaggag gctttcgtca cgccggtgat gtcgttcgtg atctcatcca
    24181 gcgtgtaccc gacggccagc ttggcggcaa tttttgcgat ggggaatccc gtcgctttgg
    24241 aggccagcgc cgagctgcgg gagacgcgcg gattcatttc gatgacgacc atgtccccgt
    24301 tcaccggatt catgccgaat tgaatattgg caccgcccgt atccacaccg atttcacgga
    24361 tgatgcgcac ggccgcatcg cgcagcatct ggtattcctt gtcggtcagc gtgagcgccg
    24421 gcgccacggt gatgctgtcc ccggtgtgga cgcccatcgg gtcgagattc tcgatcggac
    24481 agatgatgac cacattgtct ttcaggtcgc gcatcacctc gagctcgaac tctttccagc
    24541 cgatgacgga ctgttcgacg agcacctggc ggaccgggct catggccagg ccccattcca
    24601 cctgtgtccg gaactcttca atgttgtagg cgatgttgcc gccggttccg cccatggtaa
    24661 aggagggccg tacaatcgcc gggaactgcg tgcggcggac cacttcttcg gcttcttgga
    24721 gcgatgtcgc gacgccgcta tccggtacgc gcaggccgat cttccacatc gcctgccgga
    24781 atgcgtcacg atcttcggct ttatggatcg cctcgatcga cgctccgatc agcttcacgt
    24841 tgtatttttc caggacgccc cgtttggcca gaccgatggc ggtattcaac gccgtctgcc
    24901 cgcccatggt cgggagcagc gcatcgggcc gctcgtactc gatgaccttc tccaccacgt
    24961 cgagcgtgat cggttcgatg taggtccggt cggcaaaatc cggatcggtc atgatcgtgg
    25021 ccggattgct gttgatcagg atgacgcggt agccctcttc cttgagcgcc ttgcacgcct
    25081 gggttcccga ataatcgaat tcgcaggctt gcccgatcac gatggggccg gagccgatca
    25141 agagaatgga gcggatgtct gtccgtcgtg gcacaattga cctcgaataa aagaagttag
    25201 ccggtcggca gaggcgcctc gggcatgggc cccaccggag gccggtaggg ttggatcgga
    25261 ccggacggga ttgccggggc ctttcctgcg ggatgatagt gtttcatggc gtccgggatc
    25321 caggcctcga tctgattgat gcgggtgacg tcggacggat gtgtcgaaag gaactccggg
    25381 atcgcctgct gcgaacggaa gcacagcttg ttgatcatgg cgcggggaca gccgctcatc
    25441 cgttcccaga acgacaccgc ttcgcgcggg tcgtatcccg cctcggccat caggcgcaga
    25501 ccgataaaat ccgcctccga ttcctggcgg cggtcgaagg gcaaggaaac gccgacgcca
    25561 tagaccgtca tcgcagccat ggctgcatcc ggccgtccca cggcggcacc ggcccctaag
    25621 gcggccagtt gtccgatctg ttcgagaatg ccgcgactca tccgttccgc tccgtggcgc
    25681 tgcagcgcat gcgccacctc atgaccaatg acggtcgcca acccgtcttc ggtcttggtc
    25741 actttcaaaa ttccggtgaa gactgcaacc ttgcccccgg ggagcgcgaa ggcgttgatc
    25801 gtccggtcgt cctgaatgac ggcgaattcc cattggtatt ccggtttgtt cgccgccttg
    25861 gcgatgcggt tccccacccg gtgcaccatc tcgttgagct cggggttgtc gctcaagggc
    25921 gcttgccgga gcacttcccg gaaggccgag agacccatcg ccatttcttt ttcttccgac
    25981 agataaatga a
//
LOCUS       DAOQDS010000054        25897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_680_length_25897_cov_22.384142, whole genome shotgun sequence.
ACCESSION   DAOQDS010000054 DAOQDS010000000
VERSION     DAOQDS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 25897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25897
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_680_length_25897_cov_22.384142"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1290)
                     /locus_tag="PK589_13450"
     CDS             complement(169..1290)
                     /locus_tag="PK589_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20660.1"
                     /translation="MRNPRATIHRTNRRVGAHLVAMVLLLGLSPHPGLAFTIVAPKNG
                     TTLTSGEPTTAEVEAGREAGLVEVRYYWYPEQTESLVEQGDERSGKGSQGSISTEKYW
                     QKDSITGAPVVALPALTSTVDRVPPYGGALPVPPDAVGRMRLLAIADISQGRLGRKTV
                     FDEVFVTVQPKAELLSIDFETEKPLQLGRTGQSSAYGHVDSLGKIFELPVVGEFADGI
                     SRPLSSPSAGTVYTSGNDQILKVLPDGLLQIVGIGKTSLTVTNRGKQATLDVRVEVNP
                     EPNEPPVANAGTAKTVKAGTKVRLSGLQSRDPEGEALFYAWAQVRGSKVAMLDVNMAE
                     PSFTAPYVSETRTFRFKLRVTDKKGADSVPAYVDVTVEP"
     gene            complement(1293..3107)
                     /locus_tag="PK589_13455"
     CDS             complement(1293..3107)
                     /locus_tag="PK589_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNJ20661.1"
                     /translation="MILKRWLVGLPLKTKEAAHERLSKRLALAVFSSDALSSVAYATE
                     EILLVLTLAGTAMVGYSIPLSLSIIGLLVILTMSYRQIIFEYPEGGGAYIVGKSNLGE
                     WPGLVAAGALMIDYVLTVAVSVAAGIAALTSAFPDLLAHREALCVAAILLVTVVNLRG
                     VRESGQFFAVPTYIFIGTLTAMLGVGAIQILLGHATRIEPLPSLTPAEPLTLFLLLRA
                     FSSGCTALTGVEVISNGVSAFKKPEAKNAAFTMIGMAVILGTLFIGISAMAYYFGIVP
                     KSDETVVSQIARATFGTGLLYFLVQASTMTILILAANSSFNGFPRLASILARDSYMPH
                     QMSMMGDRLVFSNGVIILGVFSCLLIILFNGDTHALIPLYAVGVFLSFTISQAGMVKR
                     WLVKKGPHWEKKLLVNGIGAVTTAIATVIIASTKFAHGAWIVIVLIPMLIMFFKAIRS
                     HYKAVSEQVALSRGHRPPLPRRNIVVLPIGGVNRAVIRAVDYARSRSGDIRAVLVDVD
                     PEETARVEIQWAQWGCGVPLTVLPSPYRSVLSSLLDYLEQVLQKDQECWVTVVIPEIL
                     PARWWQNILHNQRAFMLKGALLFKDRVILTDVPYHLTR"
     gene            3470..8335
                     /locus_tag="PK589_13460"
     CDS             3470..8335
                     /locus_tag="PK589_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNJ20662.1"
                     /translation="MDCQWFTRVTSRALSVIAGGALVCLPLAASAESQLHADHAVPQQ
                     TAMSHQLPSWAEKLKGQTIVEDAMAGKPERSAMVEQQHQRVMEHLTNDPQAQAVNTGM
                     FNTQTMMHQYGAGGQDLLLMSDPRVEPVAMTNGGKCPATAPVRQYNVSAINVEITLNQ
                     WLDFYPGYMYTLDENLDKVRAEETKNREARDKEGFDPGAVIPGVQAQWIQPLTIRGNQ
                     GDCVKIKLTNKLEGGEDVSLHIHGSSMVVSATGAAAATTNSDSIVQKDKSGEFEWYIH
                     PSTQEGVRQFHTFANDRELTVMGLFGSFVVEPRGSEYLEALGTGDATPTASGWQVIIR
                     NGTGPDFREFVLYYHEVGDEAFRPVNKKGDFLPQRDPLTDAYRPGGRAINYRSEPFGI
                     NNMHVQHEYFGFEDESMGYSSYTFGDPSPTIPRSYMGDPAKFRLVHGGSEVFHSHHPH
                     GGTIRWPRSPRAIDDMNLWTTAGNGPVKYPVIRAKTDRVDVEVIGPSEALDLETECGS
                     GLCQQLAGDFLFHCHVAHHYVAGMWGYWRVYNTLQQGDMRNDVMPDLRELPDRKGRIR
                     GPITSDKLVGQTVDWFGKSFKIVEKGKSDWKSNPAVVTIKDWVEMQLPTMGKPGHKND
                     EKGQTVSYDATVLDWAWEGTRALSEPESTIDNPKYKSTHPGKRHPITFEPLTGKVAWP
                     HLTPHFGRRVMFSPNHVGAPWLEMIRRDANGEESVDQALPGENGNWSLCPENAGRKHY
                     NVHFIKLPITIAKKTGKEPPVIDPNGLIYVLHEEEAEIRKNDDLKYPLVVRGNIYDCV
                     DWTLTSEWDDDDYTNFQSSKINTHWHFLQFDNQASDGVITGFSYEQSVRPFTMLEKKN
                     AKGLPVPMNTVVTAAAKKGANTITVKNAKQFHVGTLLLVGADNVKGNEISRIKAINGN
                     TITLAKGLKNDHPANDIVTVEFVRQRFWVDADVGTVFWHDHAFGATTWPHGGFGTFIA
                     EPVGSTYHDPKTGKLIRSGPIADIHTNEPVGHGVNNSFRELMVQVHDTVPHTVNVVTA
                     GNPPGQPIEVALEAGKTVSFMMPEKIYMTPMPFLNGGTHTTGSGLNFRAAPIAQRLAT
                     NPDASQIFSSQVHGDPYTPTLRAYVGDTMVFRLLHTLMNESMVWTLSGHTFWTERYAA
                     DANRKNSIHIGIAERYDLVVPEAGGSRHQAGDYLHFNGRSSKFSEGGWGIIRVLDKEQ
                     ADLKKLPAGFSIKGDIPKTLPVCPADAPVKQFNVVAMDYAGMKFNAKAPESIEVDFER
                     RILMTNAEAKIYALEEDTAKVAAGAHPMPLTLRVNSGDCVKVHLKNKMKDSKASFSAI
                     GLAFDPKDSMGANVGNNPGDQTVAPGGERDYTYYADPFNGETTSLVWDWGNVMTNPRN
                     GLFGAVVVGPKGSKYRDPKTGADLSNKNAWAADVIIDHSVPGNELRANYRDVALFFQD
                     EDNIIGTSFMPYVQNVAGLTGVNYRSEPYKFREEQGCSLGKVFQPCKADKPEDPATPL
                     IEAHAGDPVRIHVLGVSNEQNGMFSVEKHEWPIEPFMRGADLISVVEFSGSETIDAFL
                     PSAGGMYRMPGDYVYSNQRLPYSQSGQWGYVRVLPAGDTRLLPLTGAGAGAKSAEVEQ
                     PAAHAMPVASK"
     gene            8489..9397
                     /locus_tag="PK589_13465"
     CDS             8489..9397
                     /locus_tag="PK589_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ20663.1"
                     /translation="MRTAGIALLAALSIFSSQSWAYEEITVTDGGNITGTVTMIGGKP
                     TPKGYNLITFPDPVYCGRISTGTGWRILDEFSMTSGSGLKDVVVLLTDATKGKPFKFE
                     PVTIEARDCRFLPFVTVVKDGSEVTVMNMDPVMHDIQAYETSQLGPRVLFNTPLPMNP
                     HHKRFVTAESHEHLAGEPVKEAIHMTKGRRIFVMQCGFHAYMESWGLAVDNPYYALTT
                     DGGTFTLGDVPPGEYTLMAWHPALGTMLQKKVTVTEKGTSTVDFAFEAPKGRRSVHEI
                     EHNPHYGPESLGKVVDIRPTLERQVP"
     gene            9425..10357
                     /locus_tag="PK589_13470"
     CDS             9425..10357
                     /locus_tag="PK589_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ20664.1"
                     /translation="MMLNRTQRIHSFAAAVLMWLGVAVSPVGAYEVIDVQHGGTVDGT
                     VTLSGGIPDPKAFNLITFPDPTYCGRISNGSGWRLLRDFIVSPEGGLKNAIVTLEGVE
                     SGKAFDMSVPLIEARDCMFQPWVTIVRNGHAVEVVNMDPVMHDIQGYETSPEAGARVL
                     FNTPLILNHQHQRGNMRAVHNHAPGKSLVGPVYLNKGRRTFYMQCGFHAYMESWAMAV
                     NNPYYAVTDDQGAFKIENVPPGIYQMVVWHPQSGPGVTRTITIAPDGTTTEKVALPAP
                     KGVRTAYRAMENPRFGLESLGHPVEIQPLVEHQH"
     gene            10539..11003
                     /locus_tag="PK589_13475"
     CDS             10539..11003
                     /locus_tag="PK589_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20665.1"
                     /translation="MFAKSDRIRLEYKYAIKTELGYSSIEILRLDKRESWFLLAQRRQ
                     ILSLPIKPEEILPIQPTLPGEHSRTLIGDATTIGRASRLYEVRVDYNGRNERFYEWVD
                     VETGIVLKLVSQDRDWSIEYLRFRLSPQPDYYFEEPTGYQRWVPKPNAQERG"
     gene            11034..12431
                     /locus_tag="PK589_13480"
     CDS             11034..12431
                     /locus_tag="PK589_13480"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ20666.1"
                     /translation="MGLQTKRRWIRTVNLCLAAVIVSGPLVGLWPVPANAGSLKDAQA
                     AFDKKQYQEALDIIEQVTKEKGSQPETRRLKVRSLIFLGKPKDALVEYERHEQESKQE
                     DRPLLKDVSLGFIYALVKDMREQMRGAAYTALKDVDSAETIPALEDGLSDGSGLVRAL
                     AAEALGKLDAGRKSPRLRNALEDQAGLVKATVIKVLGKSGDRSVIPLLEKALKDEQPA
                     VRLAAAGALYHTGQTAMWETIQKAAAAPNPEERATALRMVGDLKDARGLSILLEAMTN
                     TQPSVRGAAASALGELGKVQGIPALEKALDDKIPAVKTSAAISLGELGVKESLVALRK
                     SLADPNPVVKAAVVSALLRVEEPFESVAEALYELAQNNDPGTRSAAGKAAGRAHGANT
                     QPAIEFLAGLLKDSIPRPRIAAARALGQIGGAEVLPILKAALHDEDDAVRATVGGSIA
                     RIIGHAKFPSNQAKS"
     gene            12634..13431
                     /locus_tag="PK589_13485"
     CDS             12634..13431
                     /locus_tag="PK589_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ20667.1"
                     /translation="MKKGAMKAVFGVAAAAFLAAPLTAFAGGTIAGKVTYPGKAEQKE
                     FSFAKFPNPKFCPKNPNKSLMDGDKRFLKTIEVAKDGGLKGAVVAVVDIEDQAFQDGF
                     KGTDVVAEFCEFLPFSGVVVNNKNFRAENKDADPDDPKSVQGVLHNPHSFTVKGSTSA
                     TGFNIGLAKKGDKLEKPVTFRGGAEKAGYYRLQCDQHEFMQSFFLPVWNPYHAVVKDD
                     GSFEIPGVPAGKHKVVVWHPFVGKGKLNEFEIEVAEGGTANLKAEIK"
     gene            13708..14703
                     /locus_tag="PK589_13490"
     CDS             13708..14703
                     /locus_tag="PK589_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNJ20668.1"
                     /translation="MPRELSLAEVFQLGYYWETKILLTAVKLDVFSLLDGQGRSAAEV
                     AQKLGADSRALELLLNALVAIRLLTKTGAVYANTSVATTHLVKHGPQYIGHLLLLHDA
                     EWGNWGKLEEAIKTGRSPVTQHVFETDPALGANVLSVLHRIGQQSGPDLAKRLALGQA
                     KTMLDLGGGAGTNAIAFCQVYPSLSATVFDLATTLPVTERTIKDAGLEGRIALKAGDF
                     NRDALGGPYDVVLMSDILHYQDLAINAALVKKIHSHLNPGGRLIIKDRFLDASGTSPA
                     WTAAFAVHILVNTEQGACYRTAEAMQWMHDGGYVSVEEIERTAVVQGVKPSAGQS"
     gene            14706..15422
                     /locus_tag="PK589_13495"
     CDS             14706..15422
                     /locus_tag="PK589_13495"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNJ20669.1"
                     /translation="MFEFLRQPGFFGTHATMGADLSQLMATLFTGLFIIGWIQAKQHR
                     GHPHHWLMLGGMITMVGFFTAYYLFRQLGVLAFEGKEGFGGSQALYDYVFIPVLTIHI
                     ILVIIGLVMAVYMIVLGFRSQQFIGGSRVLSPSLLQTSWKKVGLIFGVLLTVVIVLFG
                     TRVMSAGFSMRKLEVYLGFLSLVAIVFAVEMGIQRIWPDGGQRHRALGRFTMIVYCIL
                     FLTGTFTYAMLYILYPGKIG"
     gene            15455..15949
                     /locus_tag="PK589_13500"
     CDS             15455..15949
                     /locus_tag="PK589_13500"
                     /inference="COORDINATES: protein motif:HMM:NF019968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HNJ20670.1"
                     /translation="MLVCGCGGWMHTEGIEERRSHGGDATWFVRTECRPCRWLVGIDM
                     PAGQVDGLVDRLMWSDDAKHRLDRVPPYAAPALRELVEGFARSRRHRVITYELIDQAL
                     TGDVVAWDADAEQRLANVPAPVRAMARIELERTAVDRGERSVSVALMEEVKARYFGMA
                     AQRA"
     gene            16031..17116
                     /locus_tag="PK589_13505"
     CDS             16031..17116
                     /locus_tag="PK589_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20671.1"
                     /translation="MLHRLALRVLPSLTLAVTEDSVRKRKRLVMFVPGLAAFAVYRAA
                     KQGFSLADPLTLLVLCGLVSMATALCAYRVGRGVPFRQLVTADGWQRIGWVLGWIGFV
                     YGIQLSLLVLALLWLVGYDYAKHPDGPAMMALIIPSTAVARDAFEIGYVRWLEACGRP
                     FITFPDGVALRNLLQRFDQALLTWVTAGVLGGVVLSALAAMAVSGDFAVLAQGGLVTL
                     AAGTVGLLAYFAGQGDQATLTSRLAATSWGELMKFWWWPGLAFAATYYLVLVGGCLYV
                     LKQPMNSIVLFAGVAALVGGVMPLYCYYLGYRREIESRQPGGVPSALLRCPFVMGILG
                     KSREAIVGSALSESAAVFEKSSQRVSS"
     gene            17113..18981
                     /locus_tag="PK589_13510"
     CDS             17113..18981
                     /locus_tag="PK589_13510"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNJ20672.1"
                     /translation="MTTRRSLSFVILLLVGGLLSVASLIVLSPPSIMAADPSNDVYFH
                     TPGTPSGPSAPTANDNHYPQVGSMDSRLLMWFIIQQHTYFGGFVLALPVFCVLLEFLG
                     LVARNPAMALRYDGLAQDLLKVALLALSVTAAVGSLMLTMFITLYPSFMQYMGGTFKG
                     MMPLYALVFVGTTFLTIAYYYSWERMAAPGTKWVHLSIGLLAVVCGTSLLLLANSWSA
                     FMMAPSGVDGQGRYLGNPWHLLRSALWNPLNLHRFLADIMSGGAVVLAYACYRFFSGK
                     SQEERAYYDWVGYVFLFVTVCALLPMPFAGYWLMRSVYAYSQSMGVTMMGGLLTWLFV
                     VQALLIGALFLGVNYYLWQSMGRIRGGERYQPYYQYLLLALTGCLFIWLTPHTLLMTG
                     TEVKAMGGAQHPVIGNYGVMSSKNGAVNVMICITALSYIYYRRANRTMTISWARVGNV
                     ILGALFGLGVAHIIGLSIYGFYLPASLRVGLSGPQAVTTLLVVTLGLLLNRRMLQGAT
                     IHGPVQWGQISVRGMVGLFGLAAAFTWVMGLMGYIRSSGRLAWHVNELMPDTSPWAFT
                     PTLGFAAKMVTINMVLFWGAVFFLFWVCQRGQQPVMHEDVSAEKEAGFLPSPSHEG"
     gene            19007..19669
                     /locus_tag="PK589_13515"
     CDS             19007..19669
                     /locus_tag="PK589_13515"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HNJ20673.1"
                     /translation="MGMRKLVGALGLLSGIALCSILGGAAPASAAETGVMVLEDFQSA
                     DQDGFPVDWQHENQRSQAKGRDAYKLQSEEGKKFLAAKDAGQRIKKRKIDWDPKVYPV
                     LTWRWRLHKAPSNSEPVAALYASLDTDLMFIPVFTKYIWSAGKPVGTFVEGGMFSGSE
                     LVVQSGVVPVGEWVEERVNVYEDFKRIHKHEPADKAWGISLFAGPGVEIDFGAVTVGP
                     AK"
     gene            19702..20091
                     /locus_tag="PK589_13520"
     CDS             19702..20091
                     /locus_tag="PK589_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20674.1"
                     /translation="MDRPTANTWFDVVLGIVVMATVGALIGSFLGASSIPVTAGLGLA
                     LGAVVGYLGGRRFLVSILVGTVLGGLLAWLIAGVEKVSFGAGAGAAMGGFLGVQISML
                     LDVRAARKAAQLEESEADATHSAVTKL"
     gene            20107..21021
                     /locus_tag="PK589_13525"
     CDS             20107..21021
                     /locus_tag="PK589_13525"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNJ20675.1"
                     /translation="MENRGVLIGSIIFVFGSFILMIGGLVYESYKAKQMRQLALSIAT
                     EAKPVAAPLAQDFSMYKTFIGDDGREMVQISEGPFVMGSRDNDSDPDEKPEHQVYLKT
                     YFLDKHEVTQDSYDRFLKMTRRVKRKIEVFEDDPAKLLKPEYPAIAVTWDDAEAYCKW
                     AGKRLPTEAEWEKAARGEGKRRYAWGEEFISGYANIDGAEDGFRYLAPPGSFESGRSP
                     YGIYDMTGNVGEWVADAYDENFYRASPYRDPKGPDQGEQRIIRGGSWRETKRNVRASK
                     RFQAKPWRHDITVGFRCAKDVDADTAVK"
     gene            21033..21986
                     /locus_tag="PK589_13530"
     CDS             21033..21986
                     /locus_tag="PK589_13530"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ20676.1"
                     /translation="MLETRFKVVFLLAVLFAASLPIIAILRGTISTPLESEVQHSEED
                     SQSSASDSSIPDQPLEEELVRIPAGPFIRGTSQGGFDEQPERKIYLDEFFIDRYEVTN
                     AQYAAFVKATGHRKSGPPSRYAKNTGRMKGVNQPAVYVSWEDAKAYCEWRGRRLPTEA
                     EWEKAMRGTDGRLWPWGNVEMPGGANWGRINDGFEVTAPVGRVRSDASPYGVMDGAGN
                     VMEWVEDWYLEGAYAAAAERNPESPEYGTYKVLRGAGYTSSGSDLRITARSKMMADFR
                     DETIGFRCAQSKAGNAPLSGGVNDGKIIENQSSRGSETRPK"
     gene            22312..22434
                     /locus_tag="PK589_13535"
     CDS             22312..22434
                     /locus_tag="PK589_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20677.1"
                     /translation="MGQTIVYFVVSILICVSFFAAVDHFLMDAQGLDFWYLLRK"
     gene            22498..24414
                     /locus_tag="PK589_13540"
     CDS             22498..24414
                     /locus_tag="PK589_13540"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNJ20678.1"
                     /translation="MGSVTRTKLMSVMALCAMIGLLLMPILVAIPALADGGAPAADAK
                     KEGGEAKVEKGKDVYYKTEGIVSGPPAPKTTDSEKDYPRYNFESRVLLWFANQQHLYY
                     GSFVLAVPIFCMVIEFMGVVTKDKALAKRYDQLAYDFIKISLTAYSLTAILGGILIFT
                     FLTLYPAFFQYLSGIFRPVMHIYALMFVAESGTLYIYYYGWDKMKEGFLKWIHLSMSV
                     VLNVIGTILMFLANSWIGFMMSPAGVDEQGRYLGNIWHVIHTALWNPLNLHRILGNMA
                     FGGGVVAAYAAYRFLSAKTDEERAHYDWMGYIAMALGVAFLIPLPFAGYWLMREVYAY
                     RQQMGITLMGGLLAWLFIIQATMIGILFLSTNYYLWQALGRMRGAEKYQRYIKYLVFL
                     LTCGYLVFITPHTIVMTPAELKAMGGQQHPVLGNYGVMSAKNGGINVIITTTVLSFVW
                     YMRGNKVSTVSWAKFGNIFMGCFFFFAYLNIIMLAVYGYYIPANVRVGLSVPQVATTL
                     SCLFFMFALNSVMMKGAKQMGPIEWGKISARSQYALIMLATAFTWMMGLMGYIRSSVR
                     LFWHVNEIMRDNSPWAYTHTVGFAANMISANVLFFWISIMFVFWLGALAAKKAPVEAK
                     AAVPGRAAAPAVGH"
     gene            24500..25819
                     /locus_tag="PK589_13545"
     CDS             24500..25819
                     /locus_tag="PK589_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HNJ20679.1"
                     /translation="MIELIKSALSMGWPALGLLIGLMFYFKASISDPVANKRAVFKTF
                     IGIWGALLLFMAIANYKMNFFGESRLLPVSLVLITSMTFMMALYFTNISALLKIGGFM
                     FFIAAALSGYGNWLPQVEGGFPPIEEKKDFSNMPQTELADEGEKIIFGGVGQNKVQGA
                     IGKGQCPLCHAFHKGMLGERAPNLDGIPERAETQINDPRYHKGNPAARDSNQKEAFAG
                     SGTAENGIQYIAESHACPSCFVVAGFGVKGTNDKESPMPSIHKPPISLSLPELAAVDT
                     WLYVREGREAPGFDEIVKSYEKFIPESDRPKPPTEGDKAAGGGGASLMADGTEPVDQI
                     FAKGQCVACHTIPGIAGATGTIGPKLVEGTNAPLRIKDKDYKGKAKSVPDYIMESIVE
                     PSAYVVKGFPDNTMPKVFGQKLSAGALKKLVDYLSQIQEGKEPPKAS"
BASE COUNT         5471 a         6615 c         7999 g         5812 t
ORIGIN      
        1 aaggctgcaa ggtctgtatc ggagaagata cgagcgtatc cccgcagata cagagaaaca
       61 tcatcccccg aggccgcagg caggaagcaa ccggaggcgt cgcctttggc tacgttgacg
      121 atcggttcga gccgggaacg aagtcgggga tcattttttc agcagccgtt agggttcgac
      181 agtcacgtcg acataggccg gcacgctatc cgcgcctttc ttatccgtca cgcggagttt
      241 gaaacgaaaa gtgcgggttt ccgacacgta cggggcggta aacgacggtt ccgccatatt
      301 cacgtccagc atggccactt tactcccgcg cacctgtgcc cacgcataaa acagcgcttc
      361 tccctccggg tcgcgacttt gcaatccgct taagcgcacc ttcgtgccgg ccttcactgt
      421 tttggcggtc ccggcattgg caacgggtgg ttcattcggc tccggattga cctccacgcg
      481 tacgtcgagt gtggcctgct tgccgcgatt ggtgacggtg agcgaggtct tgccgatgcc
      541 gacaatctgc aacaatccat cgggcaggac cttgaggatc tgatcgttcc cggatgtata
      601 gacggtgccg gcgctgggag aggacagcgg gcggctgatg ccgtctgcaa attcgcctac
      661 caccgggagt tcgaagatct tgcccagtga atcgacatgg ccataggcgg aggactgtcc
      721 tgttcgacca agttggagcg gtttttccgt ttcaaaatcg atcgagagaa gctcggcctt
      781 tggctgaacg gtgacgaaga cttcatcgaa gacggttttg cgtcccaggc ggccttgtga
      841 aatatccgcg atggccagga gccgcatccg gccgacggcg tcaggcggaa caggcaatgc
      901 gccaccatag ggcggcaccc ggtccaccgt tgaagtcagc gccggcagcg cgaccacggg
      961 cgctcctgtg atgctgtctt tctgccagta cttttctgtt gagatgctgc cctgggagcc
     1021 ttttccagac cgctcgtcgc cttgctccac gagagattcc gtctgctccg gataccagta
     1081 gtagcgcact tcgacgaggc ccgcctcccg ccctgcttcg acttcggcgg tggttggctc
     1141 gccggacgtc aacgttgtgc cgtttttagg agcgacgatc gtgaaggcca accccggatg
     1201 gggagagagt cccagcagga ggaccatggc gaccaggtgg gcgcctactc gccggtttgt
     1261 acgatggatt gtggctcgtg gatttcgcat gctcacctcg tcaggtgata aggcacatca
     1321 gtcaggatca cgcgatcttt aaacaagagg gcccctttga gcatgaaggc ccgctgattg
     1381 tgcaggatat tttgccacca ccgggcagga agaatttccg ggatcacgac ggtcacccag
     1441 cattcctggt ccttttgcag tacttgttcg aggtaatcca atagggaact caggacggac
     1501 cgataggggg acggcagcac cgtgagggga acaccgcaac cccactgggc ccattggatt
     1561 tccacccgcg cggtttcttc aggatcgaca tccaccagga cagcccgaat atctccggac
     1621 cggcttcgcg catagtctac tgcgcggatg acggcgcgat tgacgccacc gatcgggagc
     1681 accacgatat tgcgccgggg cagagggggg cggtggcctc gggatagagc cacctgttcc
     1741 gaaacggctt tatagtggga gcgaatggcc ttgaaaaaca tgatcaacat cgggatcagc
     1801 acgatgacga tccatgcgcc atgggcgaac ttggtgctgg caatgatcac ggttgcaatg
     1861 gcggtcgtga cggcaccgat accattgacc agcagcttct tttcccagtg cggtcccttc
     1921 ttgacgagcc agcgtttgac catcccggcc tgtgagatcg tgaaggacag gaacacgccg
     1981 acggcgtaca gcggaatgag agcatgggtg tcgccgttga agagaatgat cagcaggcag
     2041 gaaaacacgc caagaatgat cacgccgttg gagaacacga gccgatcgcc catcatggac
     2101 atttgatgcg gcatgtagct gtcacgagcg agaatcgagg ccagacgggg aaatccgttg
     2161 aagctgctgt tggcagccag gatcaaaatg gtcatggttg aggcctggac gaggaaatat
     2221 agcaggccgg ttccgaaagt ggcgcgcgcg atctgagaga cgacggtctc gtcgcttttc
     2281 ggcacaatgc cgaagtagta ggccatggcg ctgatgccga tgaacagggt tccgagaatc
     2341 acggccatac cgatcatggt gaaggcggcg tttttggcct ccggtttctt aaacgcggag
     2401 acgccgttcg aaatcacttc aacgccggtc agcgccgtac agccggacga aaaggcgcgc
     2461 aggaggagaa acagcgtcaa tggttcggcc ggagtcaggc ttggcagcgg ttcgatcctg
     2521 gtcgcgtggc cgagcaggat ttgaatagcg cccacgccga gcatggctgt gagtgtgccg
     2581 ataaaaatat aagttgggac ggcaaaaaac tgtccggact cacggacgcc gcgcagattg
     2641 acgacggtga ccaggaggat cgccgccacg cagagggcct ccctatgcgc gagcaggtcg
     2701 gggaaggcag acgtgagcgc cgcgatgccg gccgcgacgc tgactgcgac cgtcaacaca
     2761 tagtcaatca tgagcgcgcc ggccgcaacg aggccgggcc attctccaag gttggacttg
     2821 ccgacgatgt aggcgccgcc accctccgga tattcaaaga tgatctggcg gtacgacatg
     2881 gtcagaatta ccagcagacc gataatcgaa aggctgagcg ggatggaata gccgaccatg
     2941 gccgtcccgg ccagggtgag gacgagcaga atctcttccg tggcgtaggc cactgaagat
     3001 aaggcatcag aggaaaagac ggctagggcg agtcgcttag aaaggcgttc gtgagcggct
     3061 tccttcgtct tgagtggcag gccaacaagc cagcgtttca agatcatgcg acattatgcc
     3121 tcaactggcg gaaaagagac aatggagcgt gacgattccc gctgagtcat cgaccgaagt
     3181 tcaattgtcg ccttccgatg tctcatggtg ccagactgat acggagaagg agtgtgaatc
     3241 actgagggcg taggtgacac tcaccctcgc gacgggggca agggggttga cagagacggg
     3301 tgagttcagt aatctccggc ccttgtctca ggggagtgcc ctgcgggcgt gaaaccgcag
     3361 gattacaacc agaacgagtc gctttttatc gaggttgtca tgttctagtt cgtaagcgct
     3421 caacatttac atcagtttct atccaaactc tatccaagga ggccggtcca tggattgtca
     3481 gtggttcact cgcgtcacat cgcgcgccct cagcgtcatt gctggcggcg cgcttgtgtg
     3541 cctgccgcta gccgcgtcgg ctgaaagtca gctgcacgcg gatcatgcgg tgcctcaaca
     3601 aacagcaatg tcccatcaac tcccgtcgtg ggcggagaag ttgaaagggc aaacgatcgt
     3661 cgaagacgcc atggctggaa agccggagcg gtctgcgatg gtcgagcagc agcaccagcg
     3721 ggtcatggag catttgacca atgatccgca ggcgcaagcg gtcaacacgg gtatgttcaa
     3781 tacccagacc atgatgcacc aatatggggc gggcggtcag gaccttctcc tgatgtctga
     3841 cccgcgagtg gagccagtgg caatgacgaa tggcgggaag tgccccgcca ctgcgccggt
     3901 ccggcaatat aatgtgtcgg cgatcaacgt cgagatcaca ctcaaccagt ggctcgattt
     3961 ttatcccggc tacatgtaca cgctggatga aaacctcgac aaggtccggg cggaggagac
     4021 gaagaatcgg gaagcccgcg acaaggaagg gtttgatcct ggggcggtga ttcccggcgt
     4081 acaggcccag tggattcagc cgctgacgat tcgcggcaac cagggtgact gcgtcaagat
     4141 taagctgacc aacaagttag agggtggcga agacgtcagc cttcatatcc atggctccag
     4201 catggtggta agcgccaccg gtgcggcggc cgccacgacg aactccgaca gcatcgtcca
     4261 gaaggacaag agcggcgagt tcgagtggta cattcacccg agcacgcagg aaggtgtccg
     4321 gcagttccat acgttcgcca atgaccgaga attgaccgtg atgggtctct tcgggtcgtt
     4381 cgtggtcgaa ccccgtggat ctgagtacct ggaagccttg ggtaccggcg atgcgacccc
     4441 gaccgcgagc gggtggcagg tcatcatccg gaacggcacc ggtccggatt tccgcgagtt
     4501 cgtgttgtac taccacgaag tcggtgacga ggccttccgc cccgtcaata agaagggcga
     4561 cttcctccct cagcgtgacc cgttgacgga tgcctaccgt ccgggtggcc gcgcgatcaa
     4621 ctatcgcagc gagcccttcg gcatcaataa catgcacgtg cagcacgagt acttcggctt
     4681 cgaagacgag tcgatgggat atagctcgta caccttcggt gatccatcgc ctacgattcc
     4741 tcgttcctac atgggcgatc cggcgaagtt ccgcctcgtc catggtggat cggaagtgtt
     4801 ccatagccac catccgcatg gcggcaccat ccgctggccg cgcagcccgc gcgccatcga
     4861 tgacatgaat ttgtggacga ccgccggaaa cggaccggtt aaatatccgg tgatccgtgc
     4921 caagacggac cgcgtcgacg tcgaagtcat cgggccgtca gaagccttgg acctcgagac
     4981 agagtgcggc tcgggcttgt gccagcagtt ggccggtgac ttcctgttcc attgccatgt
     5041 ggcccaccat tatgtggcgg gcatgtgggg atactggcgg gtgtacaaca cgctgcaaca
     5101 gggtgacatg cgtaatgacg tcatgcctga tctgcgcgag ttgcctgatc gaaagggccg
     5161 catcaggggt ccgatcacct cagacaagtt agtcgggcag accgtggatt ggttcggcaa
     5221 gtccttcaag atcgtcgaga agggcaagag cgattggaag tccaatccag ccgtcgtcac
     5281 gatcaaggac tgggttgaga tgcagctccc gacgatgggc aagccgggac acaagaacga
     5341 cgaaaagggc cagaccgttt cctacgatgc cacggtgttg gattgggcct gggaaggcac
     5401 ccgtgcattg agcgaaccgg agagcaccat tgataatccg aagtataagt ccacccatcc
     5461 gggcaagcgg cacccgatca cgttcgagcc gctgaccggt aaagtggcat ggcctcacct
     5521 cacgccgcat ttcgggcgac gggtcatgtt ctccccgaat cacgtgggcg cgccctggtt
     5581 ggagatgatc cgccgtgacg ccaacggtga ggagagtgtc gatcaggcgt tgcctggtga
     5641 gaacggcaac tggagcctct gtccggagaa tgccggtcgg aagcactaca atgtccactt
     5701 catcaagctg cccatcacca ttgctaagaa gacggggaaa gagccgccgg tcatcgatcc
     5761 gaacggcttg atctacgtct tgcatgaaga agaagctgaa attcgtaaga acgatgattt
     5821 gaagtatccg ctggtcgtcc gcggcaacat ctatgattgc gtggattgga ccttgaccag
     5881 cgagtgggac gacgacgact acacgaactt ccagtcgtcg aagatcaaca cccactggca
     5941 cttcctgcaa ttcgacaacc aggcgtcaga cggcgtgatc accggcttct catacgaaca
     6001 gtcggtgcgt ccgttcacga tgctggaaaa gaagaatgcg aaggggttgc ctgttccgat
     6061 gaacacggtc gtgaccgccg ccgcgaagaa gggcgcgaac acgatcacag tcaagaatgc
     6121 aaagcagttc catgtcggga cattgctgct cgtgggtgcc gacaatgtga aggggaatga
     6181 aatctcccgc atcaaggcca tcaacggcaa tacgatcacc ctggccaagg ggttgaagaa
     6241 cgatcacccg gccaatgaca tcgtcacggt cgagttcgtg cgtcagcggt tctgggtcga
     6301 tgcggacgtg gggaccgtgt tctggcacga ccacgcgttc ggtgcgacca cttggccgca
     6361 cggcggcttc ggcaccttca tcgccgagcc ggttggatcc acctatcatg atccaaagac
     6421 tggaaagttg attcgtagcg gaccgatcgc ggacattcac acgaatgagc cggttggtca
     6481 cggcgtgaac aacagcttcc gtgagttgat ggtgcaagtg cacgacaccg tgccgcacac
     6541 cgtcaacgtc gtgacggcgg gcaatcctcc cgggcagccg attgaagtgg cgctcgaggc
     6601 aggaaagacc gtgtccttca tgatgcccga gaagatctac atgacgccga tgccgttcct
     6661 gaatggtggc acgcacacca ctggcagcgg attgaacttc cgggcggcgc cgatcgcgca
     6721 gcgattggcg accaatccgg atgcgtcaca gatcttcagc agccaggtgc acggcgatcc
     6781 gtacacgccc accttgcgcg cctatgtggg tgacacgatg gtgttccgtc tgttgcatac
     6841 cctcatgaac gagtcgatgg tctggaccct gtccggccac acgttctgga ccgagcggta
     6901 tgccgcggat gcgaaccgga agaattcgat tcatatcggt atcgcggagc ggtatgacct
     6961 tgtggtgccg gaagcaggtg gatctcggca tcaagccggt gattaccttc acttcaacgg
     7021 ccggtcctcg aagttctccg agggtggctg gggcatcatc cgggtcttgg ataaagagca
     7081 ggctgatttg aagaagctgc ccgcgggctt ctccatcaag ggcgacattc cgaagacctt
     7141 gccggtctgt ccggctgacg ctcctgtgaa gcagttcaat gtggtggcga tggactatgc
     7201 cggcatgaag ttcaatgcca aggcaccgga atccatcgaa gtggactttg aacgacggat
     7261 cctcatgacc aatgcggaag ccaagatcta cgcattggaa gaggacactg ccaaggtcgc
     7321 cgccggcgcg catccgatgc cgctgacgct tcgcgtgaac agcggtgact gcgtcaaggt
     7381 gcatttgaag aacaagatga aggacagcaa agcctccttc tctgcaatcg gcctggcctt
     7441 cgatccgaag gattccatgg gtgcgaacgt agggaacaat ccgggagacc agaccgtggc
     7501 tccgggaggc gagcgtgact acacgtatta cgcggatccg tttaacggcg aaaccacctc
     7561 gctggtgtgg gattggggca atgtgatgac caatccgcgc aacggattgt tcggtgccgt
     7621 cgtcgtcggt cccaagggat ccaagtatcg tgatcccaag accggcgcgg atctgtcgaa
     7681 caagaatgcc tgggcagccg atgtcatcat cgaccattcg gtgccgggca atgaactgcg
     7741 ggcgaattat cgtgacgtgg cgttgttctt ccaggacgaa gacaacatca tcggcacgag
     7801 cttcatgccc tatgtgcaga acgtggcggg tttgaccggc gtcaattacc ggtcggaacc
     7861 atacaagttc cgtgaagagc agggttgttc gctgggcaag gtcttccagc cttgcaaggc
     7921 ggataagccg gaagatccgg ccaccccact catcgaggcg catgcgggag atccggtgcg
     7981 tatccatgtg ctgggtgtca gcaatgagca aaacggcatg ttcagcgtgg agaagcacga
     8041 atggccgatc gagccgttca tgcgtggcgc tgacctgatc agtgtcgtgg aattctccgg
     8101 ttccgagacg atcgatgcct tcctgccctc agcgggtgga atgtatcgga tgcccggcga
     8161 ctatgtgtat agcaaccagc ggttgccata ctcgcagtcc ggtcagtggg gctatgtgcg
     8221 ggtgttgcca gcaggcgata ccagactgtt gcctttgacc ggcgccggtg cgggagccaa
     8281 gagtgccgaa gtcgagcagc ctgctgcgca cgcgatgcct gtcgcgtcga agtaagcagg
     8341 tcaatctcac gcgaggtgag aaagggggga gcctgaaagg gctcccccct tttgttatga
     8401 gatggggatt gtgtatgatc agctcagtgt ccgagatcat ttcgactgag actttctgac
     8461 caacccatca tcgttggagg attgatacat gcgaacggcc ggaattgctt tgcttgctgc
     8521 tctcagcatt ttctcttctc aatcatgggc ttacgaagaa ataaccgtca cggacggtgg
     8581 caatatcacg ggcacggtga ccatgatcgg ggggaaaccg accccgaaag gatataacct
     8641 cattaccttt ccggatcccg tgtactgcgg ccgtatctcg accggaacgg gctggagaat
     8701 tctcgatgag ttctcaatga catccggaag cggtttgaag gacgtggtgg tcctcttgac
     8761 tgatgccacc aagggcaagc cgttcaaatt cgaaccggtg acgattgagg cgcgtgactg
     8821 ccggtttctc ccgtttgtca cggtcgtcaa agatggctcc gaggtgacgg tcatgaacat
     8881 ggacccggtc atgcatgaca ttcaggccta tgagacgtct cagctcgggc cgcgtgtgct
     8941 gtttaatacg cctctgccca tgaatcccca tcacaagcgc tttgtcaccg cggaaagcca
     9001 tgagcatctg gcgggggagc cggtgaaaga agccattcac atgaccaagg gacggcggat
     9061 tttcgtaatg cagtgcgggt tccacgccta catggaaagt tgggggctgg ccgtcgacaa
     9121 cccctattac gcgctgacga ctgatggcgg cacgttcacc ctcggtgatg tgcctccggg
     9181 cgaatacacg ctgatggcct ggcatccggc cttgggaacg atgctgcaga agaaggtgac
     9241 ggtgacggag aagggaacgt ccacggtcga tttcgcgttc gaggccccga aaggccggcg
     9301 cagcgtccac gaaatcgagc ataatcccca ttatggaccg gaatcccttg gcaaggtcgt
     9361 ggatattcgc ccgacgttag aaaggcaggt tccgtagcag cgcgaatcgc agacaaggaa
     9421 ggtcatgatg ctgaatcgaa ctcagaggat ccattccttc gcagcggctg tgttgatgtg
     9481 gctgggggtg gccgtgtcgc cggtgggggc ctatgaggtg attgatgtgc aacacggcgg
     9541 taccgtcgat gggacagtga cgctgtctgg cgggattcca gaccccaaag ccttcaatct
     9601 catcaccttt ccggacccga cgtactgtgg gcggatttct aacggaagtg gatggcgttt
     9661 gctgcgcgat tttattgtct cccccgaggg aggactgaag aacgccattg tcacgctgga
     9721 aggagtggaa tcggggaaag cattcgacat gtccgtcccc ttgatcgaag cacgcgattg
     9781 catgttccaa ccatgggtca cgatcgtccg gaacgggcac gcggtggaag tcgtgaatat
     9841 ggatcccgtc atgcatgaca tccagggcta tgaaacctcg ccggaggctg gagcgcgggt
     9901 cttgttcaat acgccgttga ttctcaatca tcagcatcag cgcgggaata tgcgcgccgt
     9961 gcacaatcat gctcctggca agtccttggt ggggccggtc tacttaaaca aagggcgacg
    10021 aacgttttac atgcagtgcg ggtttcacgc ctatatggaa agctgggcca tggccgtcaa
    10081 taatccctac tatgcagtca cagacgacca gggggccttc aagattgaga atgtgccgcc
    10141 gggaatctat caaatggtcg tgtggcatcc gcagtcaggt cctggggtga cgcggacgat
    10201 cacgatcgct cccgatggca cgacgactga aaaggtagcg cttcccgcac caaagggcgt
    10261 ccgcacggcg taccgcgcaa tggagaatcc gcgatttggg cttgaatccc ttgggcatcc
    10321 cgtggagatt caaccgttgg tggagcatca gcactaatcg cggccgggcc ggcatcgtgc
    10381 catggctttc gagagggtgc ctctccatca gcctctgtct tctgtcgatc gtgcctgctt
    10441 ggggtgaaga ttcctctagc gtaccgtcgg tcacacagtc cgttgaattc tcggcatcgc
    10501 tcgtaaagcg ggtggagggt cgtcggttcg aagcccaggt gtttgccaag agtgatcgca
    10561 ttcggttgga gtacaaatat gcgatcaaga ccgaactggg ttattccagt atagaaattc
    10621 ttcgtttgga taaacgcgag tcgtggtttc tgctggccca gcgccggcaa atcctctcgc
    10681 tccctatcaa acctgaagaa atcctgccga ttcagcccac cctaccggga gaacacagcc
    10741 ggacgttgat cggcgatgcc accaccatcg ggcgagcctc caggctgtac gaagttcggg
    10801 tggattacaa cggccggaat gaacggttct atgaatgggt cgatgtggag acgggcatcg
    10861 tgttgaaatt ggtgagccag gatcgtgatt ggtcgatcga atatctccgc tttcgacttt
    10921 ctcctcaacc ggactattat ttcgaagagc ctaccgggta tcaacgctgg gttccgaaac
    10981 cgaatgcgca agagcgagga tgaacgtgag tggcacgagc aggaagggat ttcatgggat
    11041 tgcagactaa gaggcgttgg attcggactg tcaacctgtg cttggccgcc gtcatcgtgt
    11101 ccgggccgct ggtcggcctc tggccggttc ccgccaatgc tggttcgtta aaggacgcgc
    11161 aagctgcctt cgacaagaag caatatcagg aagccctgga catcattgag caagtcacga
    11221 aagagaaagg cagccagccg gagacgcgcc ggctgaaagt tcggtcgctg atttttcttg
    11281 ggaaaccgaa ggatgccctg gtcgagtatg aacggcacga gcaggagtca aagcaggagg
    11341 atcggccgct tttaaaagat gtgtcgctcg ggttcatcta tgcactcgtc aaagatatgc
    11401 gagagcaaat gcgaggcgcg gcgtatacgg cgttgaaaga tgtcgattcg gccgaaacga
    11461 tcccggcgct ggaggacgga ttgagtgatg ggtccggttt ggtgcgtgcc ctggccgccg
    11521 aggccttggg gaagctggac gcggggcgga agtcgccgcg cttgcgcaat gcgttagagg
    11581 atcaagccgg attggtgaag gccacggtca tcaaagtgtt ggggaaaagc ggtgaccgtt
    11641 ccgtgattcc gctgcttgaa aaagccctca aagacgaaca accggctgtg cgcctcgcgg
    11701 ccgcaggggc cctttaccat accggccaga ctgcgatgtg ggaaacgatt cagaaggcag
    11761 cggcggctcc aaacccggaa gagcgtgcca cggccctcag aatggtgggg gatttgaaag
    11821 atgcgcgggg gctgtcgatt ctcctggagg cgatgaccaa tacccagcct tccgtgcggg
    11881 gcgcggcggc gtcggcattg ggagagctgg gcaaggtgca gggcattccc gctttggaga
    11941 aggcacttga tgataagata ccggcagtca aaacctcagc ggcgatcagt ttgggtgaat
    12001 taggcgtgaa agagtcgctg gtggcgttac gaaagtctct ggctgatccg aaccccgtgg
    12061 ttaaggcggc cgtcgtctcg gctctgttgc gagtagagga gccctttgag tccgtcgccg
    12121 aagcactgta cgagttggcg caaaacaacg acccgggaac gcgttcggca gcagggaaag
    12181 cggcgggccg ggcgcatggc gccaatacgc aacctgcgat cgagttcctt gccgggttgc
    12241 tgaaagattc cattccacga ccgcgtatcg ccgcagctcg agccctcggg caaatcggag
    12301 gggcagaggt cctgccaata ttgaaagcgg cattgcatga tgaagatgat gccgtccgag
    12361 caacggtcgg cggatcgatc gcgcggatca ttggccatgc gaaattcccg tctaatcaag
    12421 cgaaatcatg agaggtcagg ccgcagggat ttcaagttga cagtgcgagt gggattccag
    12481 tatagagaaa tgttcagagg cctaaacgca gtccttccac ggctgcgtca tgagcggaga
    12541 acgatccaac aaggggcatt ggtcaccagc ccttgtggtt gagtggtgac gaagtagttg
    12601 gccggttaat gttcattaca aggaggcagt gacatgaaga agggtgcgat gaaagcagta
    12661 ttcggcgttg cggccgcggc atttttggct gcgcccctca ccgcgtttgc cggtggtacg
    12721 atcgccggga aagtgaccta tcccgggaag gcagagcaga aagagttctc cttcgcgaag
    12781 ttcccgaacc cgaagttttg ccccaagaac ccgaacaaga gcctcatgga tggcgataag
    12841 cgcttcctca agaccattga agtcgccaag gacggcggtc tgaagggtgc ggttgtggcc
    12901 gtggtcgata tcgaagatca ggctttccag gatggtttca agggcacgga cgtcgtggcg
    12961 gaattctgcg aattcctccc cttcagcggc gtcgtggtga acaacaagaa tttccgcgct
    13021 gagaacaagg acgctgatcc tgatgatccc aagtctgtcc agggtgtgtt gcacaacccg
    13081 cacagcttca cggtgaaggg ctccacctcg gccaccggat tcaacatcgg tttggccaag
    13141 aagggtgaca agttggagaa gccggtgact ttccgtggcg gcgccgaaaa ggctggctac
    13201 tatcggttgc agtgcgacca gcatgagttc atgcagtcct tcttcttgcc ggtgtggaat
    13261 ccgtaccacg cggtggtgaa ggatgatggc tcgttcgaga tcccgggcgt gcctgccggc
    13321 aagcacaaag tggttgtttg gcatccgttc gttggcaagg gcaagctgaa tgaattcgag
    13381 atcgaagtgg ccgaaggcgg cacggcgaac ttgaaggctg aaatcaagta gtcggtttta
    13441 ctttagcggg ataacctgct gaggaaaggg cagtggttcg aattgaacca ctgccctttt
    13501 tgtttgtctt gaatcgtacg actcctcagt aaagtagaga tagtcagcgc aatttcgacg
    13561 atgcgtcgtt ctcagtttgt tgcgatgcaa ccagcaagat tagtcgattg tcagactcgt
    13621 tcctgcccgc tcctcgcctt gcgtttccct ttctgaactc ggtaagatga ccaacttcaa
    13681 ttgcctcaat cactaagtgg gggatctgtg cctcgcgaat tgtctctcgc cgaagtcttc
    13741 cagttgggct attactggga gaccaaaatc cttttaactg ccgtaaagtt agatgtattt
    13801 tccctgctcg acggtcaggg gcgttccgcc gcagaagtgg ctcaaaaact cggtgctgat
    13861 tctcgtgccc tggagttgct gctcaacgcc ttggtggcca tccggttgtt gacgaaaacc
    13921 ggagccgtgt atgctaatac ttctgtggcc acgacccatc tcgttaagca cggacctcaa
    13981 tatatcggcc atttgctcct ccttcacgat gcggaatggg ggaattgggg caagttggaa
    14041 gaggctatca aaacaggccg atccccggtc acgcaacatg tgtttgagac ggatccggcg
    14101 cttggcgcca atgtcctgtc tgtcctgcac cggatcggcc agcaaagtgg gccggacctg
    14161 gcgaagcgac tcgcgctggg gcaggcgaag actatgctgg atctcggtgg tggcgcgggc
    14221 accaatgcca tcgccttttg tcaggtgtat ccgagcctct cagcgacggt attcgatctc
    14281 gccacgactc tccccgtgac tgaacggacg atcaaagatg caggtctaga ggggcgaatt
    14341 gcgttgaagg ccggtgactt caatcgcgat gccctcggcg gcccttatga tgtcgtgttg
    14401 atgtcggaca ttctccatta ccaagacctt gcgataaatg cggcgttggt gaagaagatt
    14461 cacagccatc tgaatcccgg tgggcgcctg atcatcaagg atcggttcct cgatgcgtcc
    14521 ggcaccagcc cggcctggac ggctgccttt gctgttcaca ttctcgtcaa tacggaacag
    14581 ggcgcctgtt atcgcacggc tgaggccatg caatggatgc atgacggcgg ctatgtttcg
    14641 gttgaggaga ttgaacggac ggcggtggtg cagggtgtga agccaagtgc cggccagagt
    14701 tgacgatgtt tgaattcctt cgacagccgg ggttcttcgg aacccacgcc acgatggggg
    14761 ccgatctcag ccaattgatg gcgacgctgt ttaccggcct cttcatcatc gggtggattc
    14821 aggcgaagca acatcgcgga catccgcatc attggttgat gctgggcggc atgatcacga
    14881 tggtggggtt ctttaccgcc tattatctgt ttcggcaatt aggagtgttg gcgttcgaag
    14941 ggaaggaagg cttcggcggg tcgcaagcgt tgtacgacta tgttttcatc ccggtgctca
    15001 ccattcacat cattctcgtc attatcgggc tggtcatggc cgtctacatg attgtgttgg
    15061 ggtttcgatc tcagcaattc atcggtggaa gccgtgtact gagcccgtcg ttgctacaaa
    15121 cctcctggaa gaaagtcgga ctgatcttcg gagttctgct gaccgtggtg atcgtcctct
    15181 tcgggacgcg ggtgatgtcg gccggatttt ccatgcgcaa gctggaggtc tatctcggat
    15241 ttctcagcct ggtggcgatt gtgtttgcgg tggaaatggg cattcagcgg atctggcctg
    15301 atggcggtca gcgtcaccgg gcgctgggcc ggtttacgat gattgtttac tgcatcttgt
    15361 tcctgacagg aacgtttacg tacgccatgt tgtatatttt gtaccctggc aaaatcggtt
    15421 agcgatcggg aacggctgag aggctggacg tcttatgctt gtgtgcggat gcggcgggtg
    15481 gatgcacacc gagggtatcg aggaacggcg gtcgcatggg ggcgacgcga cctggttcgt
    15541 ccgcacggaa tgtcgtccct gccgatggct ggtcggaatc gatatgcccg ctgggcaggt
    15601 ggatggactc gttgatcgct tgatgtggtc ggacgatgcc aagcatcgcc tggatcgtgt
    15661 tcccccctat gcggcgccgg cgctccgtga gttggtcgaa gggtttgcca gatccaggcg
    15721 gcaccgggtc atcacgtacg agttgatcga tcaggcactg accggcgatg tcgtcgcctg
    15781 ggatgcggat gccgagcagc ggcttgccaa cgtgccggcg cccgtgcgtg ccatggcgcg
    15841 tattgaactc gagcggacgg ctgtggatcg gggcgagcgt tcggtcagtg tcgcgttgat
    15901 ggaagaggtc aaggcgcgat atttcggcat ggccgcacag cgagcgtagt agtaggtgtt
    15961 gagacggtgt gcaggaggtc accaggtgtc cctcccgtga ctggctctct tgattcgggt
    16021 ggatgacact atgttgcatc ggttggctct acgcgtgctt ccgagtttga cgctggccgt
    16081 cacagaagat tcggttcgca aacgtaagcg tctcgttatg tttgtccccg gattagcggc
    16141 ctttgcggtt tatcgggcgg ccaagcaggg cttctctctc gctgatcccc tcacattgct
    16201 ggtgctttgc gggctggtgt ccatggcgac ggcgctgtgc gcctatcgag tcgggcgagg
    16261 agtgccattt cggcagcttg tcacagcgga tggatggcag cgtatcggat gggttcttgg
    16321 gtggatcggg tttgtgtatg gaatccagtt gtcactgctg gtcttggctc tgttgtggct
    16381 tgtggggtac gactatgcca agcacccgga tgggccggct atgatggcgc tcattattcc
    16441 cagtacggcc gtcgcgcggg atgcatttga aatcggctat gtccggtggc ttgaggcctg
    16501 tgggcgaccg ttcatcacct ttcccgatgg tgtggcgtta cggaatttgc tgcaacgatt
    16561 cgatcaggcg ctcttgacct gggtcacagc aggtgtcctc ggaggtgtgg tcctttctgc
    16621 gctggcggcg atggctgtca gtggcgactt cgcggtgttg gcccaaggcg ggctcgttac
    16681 cttggcggca ggcacggttg gtctgttggc gtattttgcc ggccaaggag accaagccac
    16741 cctcacctcc agactggctg ccacgagctg gggtgaattg atgaaattct ggtggtggcc
    16801 aggattggcc tttgctgcga cttattatct ggtattggtc gggggctgcc tctacgtcct
    16861 gaagcagccg atgaactcca tagtattgtt tgccggcgtg gccgcccttg tcggtggtgt
    16921 catgccgtta tattgctact acttgggata tcggcgagaa attgaaagtc gtcagcccgg
    16981 tggcgtgccg agcgccttgc tgcgttgccc gttcgtgatg ggcattctgg gcaagtctcg
    17041 cgaagccatt gtgggatcag ccttgagtga atctgcggct gtcttcgaga agagcagcca
    17101 gagagtgtcg tcatgactac acgtcgttcg ctttcattcg tgatccttct cttggttggc
    17161 gggctgctgt cagtggccag tctcatcgtg ctgagtcctc cgtctatcat ggcggcggac
    17221 ccgtcgaacg atgtgtattt ccatacgcca ggcactccca gcggcccttc tgccccgacg
    17281 gccaatgaca accactatcc gcaggtcggc tcaatggata gccggttgtt gatgtggttt
    17341 atcattcaac agcataccta cttcggcggc ttcgtgttgg ccctgccggt cttttgcgtc
    17401 ctcttggagt ttttgggatt agtcgcgaga aatcccgcga tggcgttgcg ttatgacggt
    17461 ctggcccagg atctgctgaa ggtcgccctg ctggcactct ccgtcaccgc cgcggtcggg
    17521 agtctgatgc tgacgatgtt catcacgctg tatcccagct tcatgcagta catgggcggc
    17581 accttcaagg gcatgatgcc gctgtatgcc ttggtctttg tcggcaccac cttcctgacc
    17641 atcgcctact actacagctg ggaacgaatg gcagcgcctg gaacaaaatg ggttcatctt
    17701 tcgatcggcc tgctcgcggt ggtgtgcggg acgtcattac tgctgctggc gaattcctgg
    17761 tctgcgttta tgatggcgcc ctctggtgtc gacgggcagg gacgatatct tggcaaccca
    17821 tggcacttac ttcgctctgc cctctggaat ccgctcaatc ttcatcggtt cctcgcggac
    17881 atcatgtcgg gtggtgcggt ggtgctggcc tatgcctgtt accggttttt ttccgggaag
    17941 agtcaggagg agcgggcgta ctacgattgg gtcggctacg tgtttctatt tgtcacggtc
    18001 tgcgcgttac tacccatgcc cttcgcgggc tattggctga tgcgttctgt ctacgcctac
    18061 agccagagca tgggcgtcac catgatgggt gggctactga cctggctctt cgtggtgcag
    18121 gcgctgttga tcggcgctct ctttctgggc gtgaattatt acctgtggca gagcatggga
    18181 cgcattcggg gcggtgagcg ctatcagccc tattatcaat atctgttgct ggcgttgacg
    18241 ggatgcttat ttatctggct gactccgcac acgttgttga tgaccggcac ggaagtcaaa
    18301 gctatgggtg gcgcgcaaca cccggtgatc gggaattacg gggtgatgtc ctcgaaaaac
    18361 ggcgcggtca acgtgatgat ctgcatcacg gcgttgagtt acatctatta ccgtcgcgcg
    18421 aatcgaacga tgaccatttc gtgggccagg gtgggaaatg tcattctggg ggcgctgttc
    18481 ggcctggggg tagcgcacat catcggattg tcgatctatg ggttttatct gccggcgagt
    18541 cttcgagtgg gattgtctgg tcctcaagca gtcacaacgc tgctagtggt cacgctggga
    18601 ttgttgctca accggcgcat gctgcaaggc gcaaccatac acggtccggt gcagtggggg
    18661 cagatctcag tgcgagggat ggttggcctg ttcggcctgg cggcggcatt cacctgggtg
    18721 atgggactca tggggtacat tcgttcctcc ggcagattgg cctggcatgt caacgaattg
    18781 atgccggaca cgtctccttg ggcctttact cccacgttgg gttttgcggc gaagatggtg
    18841 acgatcaaca tggtgctctt ttggggcgcg gtgttctttt tgttttgggt gtgtcagcga
    18901 gggcagcagc cggtgatgca tgaagatgta agcgcggaga aagaggcggg gtttttgcca
    18961 tctccttcgc atgaaggcta gacaaggttc atcagggagt gccgtaatgg gtatgaggaa
    19021 attagtgggt gccttagggc tgctgagcgg tattgcattg tgttcgattc tcggaggagc
    19081 tgcgcctgct tctgcagcgg agacaggcgt gatggtgctg gaagattttc agtctgcaga
    19141 ccaggatggg tttcctgtcg attggcaaca tgaaaatcag cgcagccagg ccaaagggcg
    19201 ggacgcatac aagttacagt ccgaagaggg gaaaaagttt cttgcggcga aagatgcggg
    19261 gcagcgaata aaaaaacgca aaatcgactg ggacccgaag gtgtatccag tcctgacttg
    19321 gcgatggcgc cttcacaagg cgccgagtaa ttcagagccg gttgcagcgc tgtatgcctc
    19381 gttggatacc gacctgatgt tcattcctgt ctttacgaaa tacatctgga gcgctggcaa
    19441 gccggtgggg acgttcgtcg aaggcggcat gttcagcggt tcggaattgg tcgtgcagag
    19501 tggagtggtg cctgtagggg agtgggtcga agagcgggtc aatgtgtatg aagacttcaa
    19561 acgtattcat aagcatgagc cggctgacaa ggcttgggga atttcattat ttgccggccc
    19621 cggcgtggag attgatttcg gcgcggtgac ggtcgggccg gccaagtaga acagggacat
    19681 ctgactgagg acgcgcagcg tatggaccga cccacggcca atacatggtt tgatgtagtc
    19741 ttgggcattg tggtcatggc gacggtcggc gccttgatcg gctcgtttct tggtgcctcc
    19801 tccattcccg tgaccgctgg gcttggactc gctctcgggg cggtggttgg ctatctgggt
    19861 ggacgcagat tcctcgtcag tatattggtc ggcaccgtct tgggaggctt gctcgcctgg
    19921 ttgatcgccg gagttgaaaa ggtttcgttt ggggccgggg cgggcgcggc gatgggcgga
    19981 tttcttggcg tacagatttc gatgttgctg gatgtgcgag cggccagaaa agccgcgcag
    20041 ttggaggaga gtgaggcaga cgcaactcac tccgcagtga ccaaattatg aggtgcgagt
    20101 aaggcgatgg aaaatcgagg cgtactcatt gggtcgatta ttttcgtgtt cggatcgttc
    20161 attttgatga ttgggggatt ggtctatgaa tcctacaagg ccaagcaaat gcgtcagcta
    20221 gccctctcca tcgcgaccga agccaagccc gtcgctgcgc ccctggcgca ggacttttct
    20281 atgtacaaga cgtttatcgg ggatgatggg cgagaaatgg tgcaaatctc tgagggcccg
    20341 tttgtgatgg gcagccgtga taatgacagt gatccggatg aaaaacccga gcatcaagtc
    20401 tatttgaaga cctactttct ggataaacac gaagtgacac aagattccta tgatcggttt
    20461 ctgaagatga caagacgcgt aaaacgaaag atcgaggtgt ttgaagacga tcctgccaag
    20521 ttgctgaagc cagaatatcc cgccatagcc gtgacgtggg atgatgcgga ggcctattgc
    20581 aaatgggctg gtaagcgatt gcccactgag gcggaatggg aaaaggccgc tcgcggggaa
    20641 gggaagcgcc gatacgcctg gggtgaggag tttatttccg gctatgccaa catcgacgga
    20701 gcggaagatg gatttcgtta tttggcccct ccaggatcat tcgaatcggg gcgcagcccg
    20761 tatggcattt acgatatgac aggcaatgtc ggtgaatggg tggcggatgc ctatgacgaa
    20821 aatttttacc gcgcctcccc atatcgagat ccgaaaggac cggatcaggg cgagcagcga
    20881 attatacgtg gcggttcgtg gagagaaacg aaacggaatg tccgtgcatc caagcggttt
    20941 caggcgaaac cgtggcgcca tgatatcacg gtcgggtttc gttgcgcgaa agacgtggat
    21001 gccgacacgg cagtgaaata atcagtcgga ccatgctgga aacacgtttc aaagtggtgt
    21061 ttctccttgc ggtgcttttc gccgccagtt tgcctatcat cgccattctc cgtggaacga
    21121 tttccactcc attggagtcc gaggttcagc actcagaaga ggattctcag agcagcgcct
    21181 cagattcctc tatccccgat caacctttgg aagaggaact cgtccgtatt cctgcgggac
    21241 cgttcatccg agggacgagt caggggggct tcgatgaaca gcccgaacgg aagatctatc
    21301 ttgacgagtt ctttatcgat cgatacgagg tcacgaatgc gcagtatgcg gcctttgtga
    21361 aggcgacggg ccatcgcaag tcagggcctc catcgcgata tgcgaaaaat actgggcgta
    21421 tgaaaggggt gaatcagcct gcggtctatg tctcctggga agatgccaaa gcctactgcg
    21481 agtggcgagg ccggcggttg ccgaccgaag cagagtggga aaaagccatg cggggtaccg
    21541 atggtcgcct gtggccctgg ggaaacgtgg agatgccggg cggtgcgaat tggggccgca
    21601 ttaacgatgg tttcgaggtg acggccccgg taggacgagt acgaagtgat gccagtcctt
    21661 atggagtgat ggatggggcc gggaacgtga tggaatgggt agaggattgg taccttgagg
    21721 gggcgtatgc tgcggcggcg gaacgaaatc ccgaaagtcc tgaatatgga acctacaagg
    21781 tgctgagggg agcgggatac acgagttcag gatctgacct gcgtatcacc gctcgtagca
    21841 aaatgatggc ggactttcga gatgaaacga ttgggttccg gtgcgctcaa tcaaaggcgg
    21901 gtaatgcccc tttgtctggg ggcgtgaacg acgggaaaat catagaaaat caaagtagta
    21961 gaggatcaga aacacggcca aaatgatatt gacaaccata ccggccaaaa ctataatgtc
    22021 gaacactttt gagttttttg ccataaaaat ccctttaaaa atcagttggg tgagggattt
    22081 tgattaacac agcatagttg gtgtgtcaat ttaaaatacg tacttaaacc gttgggggaa
    22141 tcaagatgga agctgctcag gacgacgtct cagttaagat cggcaaaatg attttctaca
    22201 tcaccctggc agtgagcctc tggttttttt actggttcgg agggattcag tgtccttgct
    22261 gatcctgatc ccggccttag aaaatgttta tcaagttgaa gggggtttgt gatgggccaa
    22321 acaattgtgt acttcgtcgt atcgatcttg atctgcgtct cgttttttgc cgccgttgat
    22381 cactttttga tggatgcgca agggttggac ttttggtatc tccttcgtaa gtaggtgcta
    22441 ggagataagg cttttgactt tgttgttcac atacgcgatg tatcaaggag gtaacccatg
    22501 ggctctgtaa cccgcacgaa gttgatgtcg gtcatggcgc tatgcgcgat gatcggcctc
    22561 ctcctcatgc ccattctggt tgcgatcccc gctctcgcag atggtggcgc tccggctgcc
    22621 gacgctaaaa aagagggtgg tgaagcaaaa gttgagaagg gcaaggatgt ctattacaag
    22681 accgaaggta ttgtctctgg gccgccggca ccaaagacga cggacagtga gaaggactat
    22741 ccgcgctata atttcgagag tcgggtgctt ctctggtttg ctaaccagca gcacctctac
    22801 tacggtagct ttgttctggc cgtgccgatc ttctgtatgg tcatcgagtt tatgggtgtg
    22861 gtgaccaagg ataaggcgtt ggccaagcgg tatgaccagt tggcctatga ctttattaag
    22921 atcagtttga ccgcttattc gttgaccgcc atcttgggcg gtattttgat cttcaccttc
    22981 ctgactctct acccggcctt cttccagtac ctttccggta tctttaggcc ggtcatgcac
    23041 atctatgcgt tgatgttcgt ggcggagagc ggcactctct acatctatta ttacggttgg
    23101 gacaagatga aggagggctt ccttaagtgg attcacctca gcatgtcagt tgttctgaac
    23161 gtgattggta ccatcctgat gttcttggcg aattcctgga tcggtttcat gatgtcaccg
    23221 gccggcgttg atgagcaggg ccggtatctc ggaaatatct ggcacgtgat ccataccgcg
    23281 ctttggaatc cgctcaatct gcaccgcatc ctcggcaaca tggcgtttgg aggcggcgtc
    23341 gtggccgctt acgcggctta ccgtttcttg tcggccaaga ctgatgagga acgtgctcac
    23401 tacgactgga tggggtatat cgccatggcc cttggtgtgg cgttcctgat tcctcttccc
    23461 ttcgcgggct actggctgat gcgcgaggtg tatgcctatc gtcaacagat gggtatcacg
    23521 ttgatgggtg gattgcttgc gtggttgttc atcattcagg cgaccatgat cggaatcttg
    23581 tttttgagca cgaattacta cctttggcag gcgcttgggc gcatgcgtgg cgctgaaaag
    23641 tatcagcgct acattaagta cttggtattc ttgttgactt gcggttacct cgtgtttatt
    23701 accccgcaca ccatcgtcat gactccagct gagttgaaag ccatgggtgg tcagcagcac
    23761 cctgtgttgg gtaactacgg cgtcatgtca gccaagaacg gtggcatcaa cgtgatcatc
    23821 acgaccacgg ttttgagctt tgtgtggtac atgcgaggca ataaggtctc gacggtatct
    23881 tgggcgaagt ttggcaatat cttcatgggc tgcttcttct tctttgccta cctcaacatt
    23941 atcatgttgg cggtttatgg ctactacatt ccggcgaacg ttcgtgtcgg tctgtctgtg
    24001 cctcaggtgg caaccactct gtcctgcctc ttctttatgt ttgcgctcaa cagcgtcatg
    24061 atgaagggtg ctaagcagat ggggccgatc gaatggggga agatttcggc gcgatctcag
    24121 tacgccctta tcatgcttgc tactgcgttt acgtggatga tgggtctgat gggatacatc
    24181 cggtcctcgg tcaggctttt ctggcacgtg aatgaaatta tgcgggacaa ctccccatgg
    24241 gcctacactc atacggtagg ttttgcggca aacatgattt ctgcaaacgt actgttcttc
    24301 tggatcagca tcatgtttgt gttttggttg ggtgccctag cggcgaaaaa ggctccagta
    24361 gaagcgaaag ctgctgtccc tggtcgtgct gcggcgccag cagttggtca ctaatcaaaa
    24421 cacgaacaat gaatcaatga gggatcgtct ccggttgcat ggcaaccgga ggcttcccct
    24481 cctgcaggag gtttgagctg tgattgaact cattaaatca gccttatcga tgggctggcc
    24541 agcattgggc ctattaatcg gcctgatgtt ctattttaag gcctccattt cggacccggt
    24601 ggcaaataag cgggcagtct tcaagacgtt cattggcatt tggggcgcgt tgctcctgtt
    24661 tatggccatt gctaactaca aaatgaactt cttcggtgaa tcgagactgt tgccggtgtc
    24721 gttggtgttg atcacgtcaa tgacattcat gatggccctg tatttcacga atatcagcgc
    24781 gttactgaaa atcgggggct tcatgttctt catcgctgcg gcactctccg gttatgggaa
    24841 ctggctgcct caggtagagg gtggattccc tccgattgaa gagaagaagg acttcagcaa
    24901 catgccgcaa accgaacttg ccgacgaagg tgagaagatc atcttcggtg gtgtcgggca
    24961 gaataaggtg cagggcgcaa tcggtaaggg gcagtgcccg ctctgccacg cattccacaa
    25021 aggtatgttg ggagagcgtg cgccaaattt ggacggcatt cctgagcgag ctgaaactca
    25081 gatcaacgat cctcgatacc acaaagggaa ccctgccgct cgcgattcca accagaaaga
    25141 agcgtttgcc ggttctggta ccgcagaaaa tggtatccag tacatcgctg aatcgcatgc
    25201 ctgcccgagc tgcttcgttg ttgcaggttt cggcgtgaag ggcaccaacg acaaagaaag
    25261 cccaatgcct tctatccaca agccgccaat ttcgttgagc ctccctgaat tggcagcggt
    25321 ggacacctgg ctgtacgttc gtgaggggcg tgaggctccg ggctttgatg agattgtgaa
    25381 atcgtacgag aagttcattc ctgagtcaga tcgaccaaag ccgccgacag aaggtgataa
    25441 ggctgctggg ggaggtggcg cttctttgat ggcagatgga actgaaccag ttgatcaaat
    25501 ttttgccaag ggtcaatgcg tagcctgcca tacaattcct ggcattgcag gggcgaccgg
    25561 cacgatcggt cctaagttgg tcgaaggtac caatgctcct ctccggatca aggataagga
    25621 ttacaaaggt aaggctaaga gtgtacctga ctacatcatg gaatcaatcg ttgagcccag
    25681 cgcatatgtt gtcaagggat tccctgataa cacgatgccg aaggtgtttg gccaaaagct
    25741 tagcgctggc gcactcaaga agcttgtaga ttacctttcg cagattcagg aaggaaaaga
    25801 gcctccaaag gcctcctaaa tcaagcgaat aggtatctgg tcgctattgc ggaagaaagg
    25861 gagatgagga catgaaagca ttaatgtcag tcggggc
//
LOCUS       DAOQDS010000055        25186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_706_length_25186_cov_19.646771, whole genome shotgun sequence.
ACCESSION   DAOQDS010000055 DAOQDS010000000
VERSION     DAOQDS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 25186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25186
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_706_length_25186_cov_19.646771"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2179)
                     /locus_tag="PK589_13550"
     CDS             complement(<1..2179)
                     /locus_tag="PK589_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ20680.1"
                     /translation="MNMCLFDPSKRVLVTVSLLMLCSGGPLWAAEPEVVESAEEIPVV
                     NASDVLISATRTPTSMGSVNQAVTIVSEEQLRQQLAASPSRSITQILTKLVPGMSQND
                     QSMDSGTSLKIRGRSIAILIDGVPQQSSPTTELDLAHIDPSAIERIEIIRGSTAIYGN
                     GAMGGLINIITKQPGEGKPSYYTDVQGSGSLTRVLRGLSGTVVQGFQGKKDWFDYNIV
                     GSFNHQGGMFDAEGSRTMPGTNTVGGFADTKNYNIMSKFGATFGDHRFQLTFNRKQSW
                     QDTNYLVDPNVDALPRTFARYVPGLSLADQPKLTNTQVSADYTHPHLFLDSRVHLQGY
                     YRTQQTRFPTLDSRLFGGTDVLQGYNQSDVLGTRLDITTPIPIYGKPEIVWGMDFSSQ
                     HGAQSSTLYDQSVFDGSGGRVFSKSGEVASSPSRVFNSTAVFAQGEWTPLAALVLRGG
                     VRYERVKVTSSDFTGNGSFLAASAIEFDTTVFNAGFVYHLLDPLDLFFNFSQGFNVPV
                     GSIDNALGNVPPGTAISLQRLQPQRVNNYELGLRGKWSKVQASVSGYISKSSLGLNFD
                     QFTNSLRRAPQTIYGVEATLDLQPIERVRLGGTYTFVEGDQKVGFNPDVRVPLNGFSI
                     APQKITAYLEHLTVPEWDWRNRVQVLYSGSRSRSLTAFNSGIDPTADPFGQTEYFLVD
                     LISTVKAGPGTLRFGVENLLNKQYATPFNQISFTNSFYFAGRGT"
     gene            complement(2395..2793)
                     /locus_tag="PK589_13555"
     CDS             complement(2395..2793)
                     /locus_tag="PK589_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20681.1"
                     /translation="MIDLLRIMHSSRRSDWISRLLAVMACLLIGVRLVGGFVCNTCYL
                     DFEKPASRSFHLHGGGDREPCHHGQPEASPLIQWACTVMQDESAFVLPDIPRLPVVIS
                     LFVPLVLLLVSYRTLFPIAAHGRGPPAGRF"
     gene            complement(2902..3276)
                     /locus_tag="PK589_13560"
     CDS             complement(2902..3276)
                     /locus_tag="PK589_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNJ20682.1"
                     /translation="MEREVDQINVIPLVDIMLVLLVIVLTTATFITTGQIPVNLAKAS
                     ASSDRQDRPIVVTVTAEGTVFVEDHAVTEEQLDTALAPHPRQSSVLVRADKVTRLERF
                     VAVVDRIRGLGFQQVSLEVFRA"
     gene            complement(3266..3691)
                     /gene="exbB"
                     /locus_tag="PK589_13565"
     CDS             complement(3266..3691)
                     /gene="exbB"
                     /locus_tag="PK589_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HNJ20683.1"
                     /translation="MESLKFGVEYGVIGLLGLLGVWALIIAIERWHFYRRVDPSAFVT
                     LDTFEMALTKRLVVIGTIAANAPYIGLLGTVLGIMLTFHTMGTSGAMAVSSIMVGLSL
                     ALKATAVGLLVAIPCVVMNNVLRRRVTELMTRYEARHGA"
     gene            complement(3917..5890)
                     /locus_tag="PK589_13570"
     CDS             complement(3917..5890)
                     /locus_tag="PK589_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNJ20684.1"
                     /translation="MPAIYGLVTVLLLVWSMVPVYAEPGSTSPPTATFASLPRIESIQ
                     LSPSGRHLAVLRHHDGQTFLETHTVTGQDAHPVVSTDNREYIITWFRWINDERLLVSM
                     RFAATPEATDTVETRLMAVNRDGTEQTANLLKQGGFSSLFGQKHVPQFEYQLVGMIPG
                     DPRHVLLALDLEHPNAPDVYKVDVYSGERQLVQANPSSKPDRNAISHWIADRTGAVRS
                     GVGQFQTAVHAIVRQPESTLWRELADYDLAKETGLVPLAFDADPAWLYVRDQHRGKTA
                     IFKLNVMDRAADRVLVVADPKFDLNGELVYAPGRKKVVGVRYSAADERVLFWDFDAQR
                     LQARIDRAIPGTVNVIHSSSDDGRLHVVKSSGAAQPPRWSIFDEQDGRMVLLGRSYPD
                     LETAALAAPTTTYVTARDGKELQVFLTVPKDRDPRHLPMIVFPHGGPAARMPGAFNYW
                     TQWFVSRGWAVLEPRFRGTEGYGDDVLRAGFQRWGLEMQNDLTDVVQYAVRAGIANAG
                     RICIVGSGYGGYAALMGVVKTPDLYRCAVSLGGVTDLPQLVSDGRGYLNQKPMVEARI
                     GSWWNDRDRLRETSPVAHAQGMRTPLLLMHGSMDRSVPVSQSRNLADALKAANATNAR
                     YVELPLADEALRREEDRLHVFSEMERFLTQHLD"
     gene            complement(6100..8358)
                     /locus_tag="PK589_13575"
     CDS             complement(6100..8358)
                     /locus_tag="PK589_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ20685.1"
                     /translation="MKCSDRVGIIVIGLLAVALSGGIQPPAGLAAEESQEQEAPVIAV
                     EPVEVSGKRIENVEDVKREFARRPGSNILIQEKEITESRALNLQDVLQFAPGVRFQSR
                     FGADEGQFQIRGTSLRNNFHHRGINILINGIFFGDADGFSDFESIDLLAYERIEVYKG
                     ANALRYGANSIGGAINFVPRTGYSASTLQMRMLGGSFGMVSGQVSSGKVLQPFKVGNM
                     SATMDYYISVSGNRQDGFQDNSQQARERINANIGLQLGNHQEIRAYFLQANVAERIPG
                     SLTNQQLFFNRQQTGGQSPPGAPPFFACVSSNQACNWGRYYTLQRIGIAYHNEFAPNQ
                     FFEIIPYFSNQFVDHPIFQTIRQENNNVGGEFRYVNSNSLFGKNNSFVAGFQPRYGHQ
                     RQQRFVNINGNIGAMTQNYTAKTTYFGAYAEDAFDATKDFTIVIGGRWDYTGREATVD
                     NFGPAGNPFNPATPSVPTSTQKPLQHFGAISPKLGFVYRTTPTSQLYFNASRSYEAPL
                     NVELLSALNANGSANTGFLNLDAQRAWQLELGHRGTSADKRYSWDVTVYNLEMQKEIL
                     ASNINNTGTFQNANGTRHTGVEAGGAMVLKKGLFAQGGAGKEDSLQTRLAYTWSRFKF
                     TDDVRGGGIGGPNVLIAKDGNTVAGAPEHSLNVEARYDHPSGWWIAPNVEWSLSGFYT
                     DYNNTIKNPSYALINMRSGWNIDEHWTLFAEGRNLTNKTYAGAVVVNDSLNRFANPGF
                     GISAFGGVEYKF"
     gene            complement(8434..9876)
                     /locus_tag="PK589_13580"
     CDS             complement(8434..9876)
                     /locus_tag="PK589_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20686.1"
                     /translation="MTRIAVRSSSVVSARATSLVRLLLALVLIAMASGCAGGVGDPVP
                     PSLPQYAFINGQWFDGEGFRAATWYSVEGRLTKRPPAGLVQTVDLAGLYMVPPFGEAH
                     NHNVEGPWNVQAVAQRYLHDGVFYVKNPNDVRDFAQQIKHAINMPTSIDATFAHVGLT
                     GHGGHPAALYEDVLRIGRYEPAVGPIERGWFENRSYVVVETDAEVEAKWPVILRGSPD
                     FLKVYLVHSEDDGEEAKVKPAHHRKGLHPALVPGIVARAHAAGLQVTAHVETAADFRQ
                     AIRAGVDELAHVPGWLIQRADDAERARLTEEDARLAADHKVRVVTTSVAGQAMPSIGG
                     HHQHGSHAGHGTGQPMKHNALPADAASHVLKDNLHLLQREGVTLVIGSDHAETSLAEV
                     LHLSTLHLFDNRTLLKMWCEATPAAIFPARRIGKFAEGYEASFLALAGNPIEDFDQVQ
                     AIRRRFKQGVPLDGALMKDAAWSAAGQRSQ"
     gene            complement(9873..10067)
                     /locus_tag="PK589_13585"
     CDS             complement(9873..10067)
                     /locus_tag="PK589_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ20687.1"
                     /translation="MNKQLLRVGEAADVLAVSRWTIYRWVEDGRLEGTKIGRGSLRVF
                     RASLDRLVQSNKTHEMNLSV"
     gene            complement(10133..12400)
                     /locus_tag="PK589_13590"
     CDS             complement(10133..12400)
                     /locus_tag="PK589_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ20688.1"
                     /translation="MPSIAQEETAAGAVVEKSARERDLRDQLQNILRELEELQQQPAG
                     GAAAKPIQPAQPAAVEESSASVAAEPIPQYELSDVSIVSDRVQRRPEGLSMSSTEQSE
                     TEAQPTRTMKESMESLPGVVLRQANGPRDFSMMIRGQGAKTTFAVRDIKMYEDGFIQT
                     QSDGLSRLDIHDPWFMRSVEVTRGASSSLYDNYALGGMVHFRTRRGSDINGVETFLSG
                     GSFGYHKEGIAVGQQFENLDASLFVSNVGEDGFIRNSSYNTQTINLNLRFKIDDKQNF
                     YFKAITNWLDARVPTRLTQSQFNADHRQAGGTNVVCNANTCSDAERLAQRRLDRRTII
                     GGIYERQINANTVLTIEADYDVKDINQTFSQISDNVNPNYKHYADLRHDGRLFDMPLR
                     SYVGFFLSNMEQEAQTFQNLADGFGTRGALLQNSRGTIRNVGGRFREELEFIPKVTLA
                     AGLGFEQSQLSVQSIGYTGGAVSSRAGADRSYTNWAPEMSVTWKPTDAQRHWVRASTG
                     YGIPGFANLLRDPVTGQPGTNFDLKPQKNLNFEIGTESKLTKTLTVQLVGFWTFFKDE
                     IITQAVSGTNTASVNADSSQYKGIEAFYDWRPMEGLRLSGAYTHIEAKYVNFADRLNT
                     AAGFLTRDGNKVPNVPTDVLNTKVAYDHTATGWGAWVEGSYYNSYFLNNSNTFGIPSY
                     MIANVNVHKNFDLKNSWFRFAKFYVEVDNIADKKYAASGQVIGGETAGAAAGQQAFFA
                     GYGRAIYGGVTFGLF"
     gene            complement(12585..12905)
                     /locus_tag="PK589_13595"
     CDS             complement(12585..12905)
                     /locus_tag="PK589_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20689.1"
                     /translation="MMRVTKPSIGLAAALAGLYLVLAVFSVACVVDHADPKPAAHHHG
                     GTVSHSSFCAWACQANPTSDAGPVALFLQPFFVATLVVESSHAIVAGGVGFHTASRAP
                     PVQS"
     gene            complement(12902..13432)
                     /locus_tag="PK589_13600"
     CDS             complement(12902..13432)
                     /locus_tag="PK589_13600"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNJ20690.1"
                     /translation="MRLGFVQTFALFVAIGLWCDPALADDRLAALKVGRVAPGTVAAP
                     FELTSLDGAAVDLAQLKGKVVVVNFWATWCGPCKEEMPAFERLRRQLDPERFALMTIT
                     TDLQREGIKQFLANLHVQVPVLFDEDQAVSQAYLVRALPTTVFIDPQGMLIGRAVGPR
                     DWDAPNVVHALQSLMP"
     gene            complement(13450..14724)
                     /locus_tag="PK589_13605"
     CDS             complement(13450..14724)
                     /locus_tag="PK589_13605"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNJ20691.1"
                     /translation="MPRRAKQALCAGLLFGLLPTMPTALAESPAGMQFGPKVTTEHKV
                     KSFVGPSVQIDEAGALSLAWMEENKEVRSVMYARSLEPGGPMGAPVRINRVEDIPYWR
                     QEAPALVVQGEDVFVTWGLAHPKATPEQPFATELRLSHSKDGGRTFQPSIVVNDDPGV
                     IQHTFDALHRDSEGRLHVSWIDGREGKKDPGTYVARSLDQGQTITKNAKVDEGTCVCC
                     RTAITSGPDGTVYVAWRKIFEGNVRETVVARSTDHGETFGSSVIVGHDKWVFPACPHR
                     PASMGVDWQGRLYIVWYTEGTDEIPAVYLAFSDDRGQTFSPKQQLNVSKNTFPDHPQI
                     AVDPEGRLVVIWEEQGPVKRDVVMSASTDRGATFTAPQKLNERKSQTPVVAVNRHGLF
                     AMAWMEHGMPGHKIVMQTLRMPSVTAAAAAGQ"
     gene            complement(14813..15850)
                     /locus_tag="PK589_13610"
     CDS             complement(14813..15850)
                     /locus_tag="PK589_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20692.1"
                     /translation="MWAPSRRFLVSVLGIVAVAVVSVTSIPQAYAACGAVSCFVTIGS
                     QQQVPQSGLLTMNFIYSYTPMSLPSGSTGLIAEADQIGRRIIPNHHNERSTYTNSFAL
                     DFNYGVTDRLGLQVTLPYLTRRHEHYHFHQGQNEDLSRFSDKGLGDIRVMAKYNVLAS
                     LKNMVVFGVGTELPTGDYRQTDNLGGVLEPAGQLGRGQFGLVGTMYQSYEILPHRLNQ
                     FVFASYRHTFKNNDGYQFGDEYIVNGGFNLVTLPWLVFTAQVNYRYMTHDSLSASIGS
                     GPILDRGIPNTGSSWFGAAPGVLFNIGDTWQAYFYAQVPLYRDANNNLAQATSYTFGV
                     TKYFQLFTPGA"
     gene            complement(15969..16253)
                     /locus_tag="PK589_13615"
     CDS             complement(15969..16253)
                     /locus_tag="PK589_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20693.1"
                     /translation="MPLLLVCAFLALQGIAYPSMLEHVAQHAHHEAGVHGTVMCSWMC
                     AAGQDLEPVPEVAPVTISVIALLDEIVVDSVSIESRTLWPSRGPPSSSPA"
     gene            complement(16285..16797)
                     /locus_tag="PK589_13620"
     CDS             complement(16285..16797)
                     /locus_tag="PK589_13620"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNJ20694.1"
                     /translation="MTALLVSTLVGSALAMGSRPPAAGMPASGFSLTDLQGKAHTLEQ
                     YRGKIVLVNFWATWCKPCTSEMPAMQTVYDQLRDKDFVVLAVNELEDEAKVREHIQQY
                     KHTFPVLLDRENRVANQYGVFGLPVSVFIDQQGVVQEYIKGGLLTEQKIQEIVSRIQQ
                     AAPIKAAALH"
     gene            complement(16888..18051)
                     /locus_tag="PK589_13625"
     CDS             complement(16888..18051)
                     /locus_tag="PK589_13625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNJ20695.1"
                     /translation="MDNVIVAHSGHSNQSVAGWGASLVFHACLALVALGLLPKMTVMV
                     EKEPFKWDVALVEPPREVARLEGPTPAPRVQPPTPTPVKPRSQPVRAVEPPPQPVQRQ
                     VETRVLPQAVQREVQPVVETVRPQEQIQSKQEVVQMQAKPVEPQEVHKTEPVVQAVAP
                     AEMQREVAAVVEQQVMEAAPATAQTYQAQQVVSAPAVVETVPEPVMTASAPVVHTPPA
                     VEAVPATAAPVDPAPAAESVAAAVSEPVAEPPAPVEAVVVPTAPVAAVTEPDPSTLRE
                     HQVVAQAAVARPAPKADYGWLADSLKRRVIELRHYPSTARLNGWEGKVVLKVSISNDG
                     QLKSVEVVKSSGHESLDQAAMEAVRRACPLHMKHELTAPMVVLNLPLVYSLNR"
     gene            complement(18087..18539)
                     /locus_tag="PK589_13630"
     CDS             complement(18087..18539)
                     /locus_tag="PK589_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20696.1"
                     /translation="MQVNQWLFRLGVCGLTGLVLSVSTPMVSAADTKAEKAFTIAVTD
                     AQGVETELKNGIFYWEEKMSETSFVPHELRHLPSKRGTATVNIKFDQIKQIELKPGAD
                     KTAPAMTVTLANGKSGEFVPAVSGSFKGESDFGQVEVPIGSLSKVVFK"
     gene            complement(18764..19096)
                     /locus_tag="PK589_13635"
     CDS             complement(18764..19096)
                     /locus_tag="PK589_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20697.1"
                     /translation="MRARLWFFTMRSSRFHSLVWVVVFAVVALLAVTTGSAVAHELHH
                     AAHHDAGTHGSGICAWMCATAGVHVVTPVHATHVLALSGDASFLSDIVHLPQVSALCQ
                     SRAPPLSF"
     gene            complement(19156..20238)
                     /locus_tag="PK589_13640"
     CDS             complement(19156..20238)
                     /locus_tag="PK589_13640"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ20698.1"
                     /translation="MRQRVTGSRHLLTTLPLLCVALFVTSLPESSAASSASPESARRS
                     YERGVVLFREGNADGAIEALKKALAQNPKLAEAYHVLGLIYFQSKRNPDEAIQAYKQS
                     LKLSAPSAEILNDLADVYLAQGRGTDAEQVLRQVLDIAPGNEEAHLDLARLYEERHDR
                     TNAVKMYQSLLRIRPDHAEALYHLASLYDSQGDLKQAREYLSRLTQANPGHANAWYLL
                     GRLAERSNDLNAAAAAFKEAIAVKADLVDAHYNLGFVYRSQGLLVEAEREFLEVIRYR
                     PEYAEAHLNLGMVYTSLNRLDEAEREHERAVALKPTSAEAHYNLGVFYELHRKDMGRA
                     LAQYRRYRDLGGRDERVERIIGVGGP"
     gene            20491..23196
                     /gene="secA"
                     /locus_tag="PK589_13645"
     CDS             20491..23196
                     /gene="secA"
                     /locus_tag="PK589_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNJ20699.1"
                     /translation="MLTQVLNIIFGSKNDREIKALRPIVEQINGLESSLTPLSDQALA
                     EKTQEFKKRLQDGETLEDILPEAFAVCREMSRRRLNMRHFDVQLIGGMILNKGRIAEM
                     KTGEGKTLVATLPLYLNALEGKGAHLVTVNDYLAKRDASWMAQLYHALGLTVGIIQHD
                     ASFLYDPTYEAADKRLQHLRPCSRHEAYRAEITYGTNNEYGFDYLRDNLIVSDLSQCV
                     QRPLHFAIVDEVDSILIDEARTPLIISGPTDQTTDLYYRINSIIPQLKPEHDYTIEEK
                     TKTASLTEEGNVRVEKLLGVDNLYDLQHMDLVHHVVKALQAYALYKRDVDYVVKDGEV
                     IIVDEFTGRLMPGRRWSDGLHQAVEAKEGVKIANENQTLASVTFQNYFRMYKKLAGMT
                     GTADTEAGEFAKIYNLDVNVVPTNRSMIRKDYADVVFRTEKEKFTAIVEEIKDCHERG
                     QPVLVGTISIEKSERLAGYLSRNGIKHNVLNAKFHEKEAEIIAQAGRKGAVTIATNMA
                     GRGTDILLGGNADFLFKRILYQDDTLTDARKQEIFDEIKADCEKDKQAVVAAGGLHIL
                     GTERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDDLMRIFASERVSQMMLKLGMEEG
                     VPIEHGMVTRAIANAQKKVEAHNFEVRKQLLEYDDVMNKQREVIYQHRHAVLAGEHIQ
                     QDIHDMMKDLVNAFVETYCPADQYQEEWDYNGLGEALQGQFALDITQGKGSVADHFKD
                     VGRDAMIEEIQTQVRRAYDHKEQELGSELMRYLEKMLLLQVIDHHWKDHLLGMDHLRD
                     GIGLRGYGQKDPLIEYKREGFDMFSSMMERIKSDVLERMFRVQAVRGEQPPPPAPEPI
                     PPPRMVLNRSDEPATQTVQSQGDKTGRNDPCPCGSGKKFKKCHGA"
     gene            23411..24586
                     /locus_tag="PK589_13650"
     CDS             23411..24586
                     /locus_tag="PK589_13650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNJ20700.1"
                     /translation="MSTGHPQLLAEIRAEIAASGPIPFARFMELALYHPQYGYYVRPV
                     EDPTQERIGWSGDFYTSSDVHPILGQALAKQAQQLDACLGHPNPFTVVEMGPGKGLLA
                     RDFLTACQNAPGNLSERLRYILIERSASMRALQHQHLAPWIGRAGHVTWLDRLEDLPP
                     DSVTGLFFSNELVDAFPVHRLAVVDGHPQEIYVDVHEEGFTEIHRPLSEELSAYLQNG
                     GIRLPDGYRTEINLKAVQWIKQVAQIMARGLVMTIDYGHTAEDLYGPERKNGTFLCYY
                     SQTTSEDAYERVGQQDMTAHVDFTTLAQAGQAAGLELTGFTNQMSFLIGLGAEQFLES
                     LQPESAEFYAAIHLLRPEGMGRTFKVLVQHTGMEKPELDGLRFKPFFGSILAARSAA"
     gene            24662..25033
                     /gene="ndhC"
                     /locus_tag="PK589_13655"
     CDS             24662..25033
                     /gene="ndhC"
                     /locus_tag="PK589_13655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNJ20701.1"
                     /translation="MGNEAAPLNYIPILIFILIAFAFGAVQILLGRIVRPSRPYRAKL
                     APYESGSPLFSDAKIQFPIRYYIIAMLFVIFDIEIVFMFPWAVAFKKLGLVGLVEMVV
                     FIGILIVGFWYAWKKGALEWD"
BASE COUNT         5363 a         7740 c         7067 g         5016 t
ORIGIN      
        1 tcgttcctcg ccccgcgaaa tagaagctat tggtaaacga aatctggttg aacggcgttg
       61 cgtactgttt gttcagcaga ttctctaccc cgaaccggag cgtgcccggc cctgctttga
      121 cggtgctgat cagatcgacc aggaagtatt ccgtctgacc gaagggatcc gcggtgggat
      181 cgatgccgga attgaacgcc gtcagcgagc gcgagcggct gccggaatac agcacttgaa
      241 cccggttgcg ccagtcccac tccggcaccg tcaggtgttc gaggtaggcg gtgatcttct
      301 gcggggcaat ggagaagccg ttcaacggca cgcgcacatc aggattgaac ccgactttct
      361 gatcgccttc cacaaaggtg taggtgccgc ccaggcgcac ccgttcgatc ggctgaagat
      421 caagtgtggc ttccacaccg tagatggttt gcggcgcacg ccggagtgaa ttcgtaaact
      481 ggtcaaaatt cagacccagc gaagacttgg aaatgtatcc ggacaccgaa gcctgaactt
      541 tgctccattt gccacgcaac ccgagttcat aattattcac gcgctgcggc tgcaaccgct
      601 ggagcgaaat ggcggtcccc ggcggcacat tgcccaacgc attgtcgatc gatccgaccg
      661 gcacgttgaa cccctggctg aaattgaaga acaggtccaa cgggtccagc aggtggtaga
      721 caaatccggc attgaagacc gtcgtatcaa actcgatcgc actcgccgcg aggaaggacc
      781 cgttgccggt aaagtcgctg ctcgtcactt tgacacgctc ataccgaacc ccgccgcgca
      841 agaccaacgc ggccagcgga gtccactcgc cctgcgcgaa caccgcggtg ctgttgaaga
      901 cccgcgaggg cgacgaggcc acctcgccgg acttgctgaa cacgcgtccg ccggatccat
      961 cgaagaccga ctgatcgtag agcgtcgacg actgggcgcc atgttggctg gagaagtcca
     1021 tgccccacac aatttccggc ttcccataga tcgggatggg cgtagtgatg tcgagcctgg
     1081 ttcccaacac gtcgctttgg ttatagcctt gcagcacatc cgtgcccccg aacagccggc
     1141 tgtccaaagt tggaaaccgc gtctgctgtg tgcggtagta gccctgcaaa tgcacccggc
     1201 tgtcgagaaa cagatgagga tgggtgtagt cggcgctgac ctgcgtgttc gttagcttgg
     1261 gttggtcggc caacgacaag cctgggacgt accgcgcaaa cgtccgtggc aaggcgtcca
     1321 cattgggatc caccaggtag ttcgtatcct gccaggactg cttgcggttg aaggtcagtt
     1381 ggaaccggtg gtcgccgaag gtcgccccga acttgctcat gatgttgtag ttcttggtgt
     1441 cggcaaatcc gcccaccgta ttggtgccgg gcatggtacg ggagccctct gcatcgaaca
     1501 ttccgccctg gtggttgaag ctgccgacga tgttgtagtc gaaccagtct tttttgccct
     1561 gaaagccctg caccacggtc ccactgagcc ctcgtaacac gcgggtaagc gatccactgc
     1621 cctgcacatc cgtgtagtag cttggtttac cctcgcccgg ctgcttcgta atgatattga
     1681 tgagaccacc catcgcgccg ttgccataga tcgcggtgga gccgcgaatg atttcgatgc
     1741 gttcgatggc gctgggatcg atatgagcca ggtccaattc cgtggtcggg ctgctctgtt
     1801 gcgggacacc gtcgatcaaa atcgcaatcg accggcctct gatcttcagc gacgtgccgg
     1861 aatccatcga ctggtcgttc tgcgacatgc ccggtaccag cttcgtcaaa atctgtgtga
     1921 tactccggct tggcgaggcg gccagctgct gccgcaattg ttcttccgac acaatggtta
     1981 cggcctgatt cacgctaccc atcgaggtcg gcgtgcgagt ggccgagatc agtacgtccg
     2041 aggcattcac taccggaatc tcttctgccg attctacgac ttccggttcg gccgcccaca
     2101 gaggcccccc agagcacagc atcaacaggc tgactgtaac aaggactcgt tttgagggat
     2161 caaacaaaca catgttcatg gtgcacgact tcctttcctt caccgacgca tcggcctcta
     2221 cccaaggccg acgcagcacg gacgacaccg acgagccctg cagcgatagc gaccgtcgga
     2281 tctgcttgag aacgagaacc cactcattca cgacagagcc agggcaccac tgggccacat
     2341 cggacaacac aggcgtccga cttgcggttg ttcattgatg ttgaagcgaa atgatcagaa
     2401 ccgaccggca ggagggccgc gtccatgggc cgcgatcgga aacagcgtac gataggacac
     2461 gagcagcagg actagcggca cgaacagcga gatcacaacc ggcaggcgag gaatgtcggg
     2521 caagacaaag gcagactcat cctgcatcac ggtgcaagcc cactgaatca gtggactggc
     2581 ttccggctgc ccgtgatgac agggttcccg gtcgccaccg ccatgaaggt gaaagctgcg
     2641 gctcgcaggt ttctcgaagt ccagatagca ggtattgcag acgaagccgc ccacgagacg
     2701 aacaccgatc aacaggcagg ccatcacggc gagcagacgt gaaatccagt ccgagcgtcg
     2761 agaggaatgc atgatgcgga gcaaatcgat catgaaccaa ccttcacaca ggaaggcggc
     2821 cactctacga aagggcagga aaaactgtct acagacgggt gctcacgggt gctacagcgt
     2881 gccacacaat cggtgtcgcc ctcatgcccg gaacacttcc aaacttacct gttggaatcc
     2941 gagcccacgg atccggtcga cgacggcgac aaaccgttct agtcgcgtca ccttgtcggc
     3001 tcggaccagc actgacgatt gacgaggatg cggcgccaag gcggtgtcca gttgttcctc
     3061 tgtgacggcg tgatcttcca cgaagaccgt tccctccgcc gtaaccgtca cgacgatcgg
     3121 ccggtcctgc ctgtcgctcg acgccgaggc tttggcaaga ttgaccggga tctgccccgt
     3181 cgtgatgaag gtggccgtgg ttagcacgat cacgagcagg acgagcatga tatcgaccag
     3241 cggaatgaca ttgatctgat cgacctcacg ctccatgccg cgcctcatag cgggtcatga
     3301 gttcggtcac ccggcggcgt aacacgttgt tcatcaccac gcagggaatg gccacgagca
     3361 atcccaccgc cgtggctttc agcgccagac tgaggccgac catgatgctg ctcaccgcca
     3421 tggcgccgga ggtccccatc gtgtgaaacg tcagcatgat gcccagcacc gtccccagca
     3481 aaccgatata gggtgcattc gccgcaatgg tgccgatgac caccaatcgc ttcgtcaacg
     3541 ccatctcaaa cgtgtccagc gtgacaaatg ccgacggatc gacccgtctg tagaagtgcc
     3601 atcgctcgat cgcgatgatt agcgcccaca cgccgagcag acccaagaga ccgatcactc
     3661 catactccac tccaaatttc agagactcca tggctttccg tcctcactgt tccttatcga
     3721 tgacgtccat cacgcgcggc cgggaaaagg cctgcgggcc tcacattggc ggcgctacgt
     3781 tacgatggcc tgcgcagacc gtctactgac gcgtgctcat ggctgctaca gttcggagag
     3841 acccacggag agggaaagtt gggataaagc tacgatgact agacagggga caatgtgtgc
     3901 aggacagcag ccgggctcag tcgaggtgtt gggtaaggaa ccgttccatc tcagagaata
     3961 cgtggaggcg gtcctcctcc cggcgcaggg cttcatcagc gagaggcaat tccacgtatc
     4021 gagcgttcgt ggcattcgcc gctttgagcg catcggccag attgcggctc tgtgaaaccg
     4081 gcacggaacg atccatggac ccgtgcatca agagcagagg agtgcgcatc ccttgcgcat
     4141 gagccacggg cgatgtctca cgaagccggt cacggtcgtt ccaccaggaa ccgatccgtg
     4201 cctcgaccat cggtttctga ttcaggtacc cccggccatc tgacaccagt tgcggcaaat
     4261 ccgtcacccc gcccagactg accgcacaac gatagagatc cggcgtcttc acgacgccca
     4321 tcaacgcggc gtaaccccca tagcccgacc caacgatgca gatgcgaccg gcattggcga
     4381 tgccggcacg aacagcatat tgcaccacat cggtcaggtc gttctgcatc tccagacccc
     4441 agcgttgaaa tccggcgcgg agcacgtcat ccccatagcc ctcagtgccc cggaagcgcg
     4501 gctccaacac tgcccagccg cggctgacga accactgtgt ccaataatta aacgcgcctg
     4561 gcatgcgtgc agcaggtcct ccgtgaggaa acacgatcat cggaaggtgg cgcgggtccc
     4621 gatccttggg aacggtcaga aagacttgca gctccttgcc gtcccgcgcc gtcacatagg
     4681 tcgtggtggg agcagccagc gcggccgtct cgagatcggg atacgacctg ccgagcagca
     4741 ccatccgtcc gtcctgctcg tcgaagatcg accagcgcgg aggctgcgct gcaccgctcg
     4801 acttgacgac atggagccgc ccgtcgtcgc tgctgctgtg gatgacattg acggttccgg
     4861 gaatcgcccg gtcgatacgc gcctgcaggc gttgcgcgtc gaagtcccag aagagcaccc
     4921 gttcatccgc cgcgctgtac cgcacgccga cgaccttctt gcgtccaggc gcatagacca
     4981 gttcgccatt gagatcaaac ttcggatcgg cgaccacgag cacacgatct gccgcacggt
     5041 ccatcacgtt gagcttaaaa atcgccgtct tgccccgatg ttgatccctc acatacaacc
     5101 aggcgggatc ggcatcgaag gccaggggaa caagtccggt ttccttggcc aggtcatagt
     5161 cagccagttc gcgccagagt gtggactccg gttgcctaac gatggcatgc accgcggtct
     5221 ggaattggcc cacccccgac cgcacggctc ctgtgcggtc ggcaatccaa tgggaaatgg
     5281 cgttgcggtc cggtttcgaa ctgggattcg cctgaaccaa ctgccgctca ccggaataga
     5341 catccacctt gtagacatcc ggagcattgg gatgttccag atccagcgca agcagcacgt
     5401 gccgcggatc gccgggaatc atcccgacca gctgatattc gaactgagga acatgtttct
     5461 gtccaaacaa ggaggagaag ccaccctgct tgagtaaatt ggcggtctgc tccgtgccat
     5521 cacggttcac cgccatcaat cgagtctcaa ccgtgtctgt cgcctccggg gtagcggcga
     5581 accgcatgct caccagcaga cgctcgtcat tgatccaacg aaaccaggtg atgatgtatt
     5641 cgcgattgtc cgtagaaacg accgggtgcg catcctgtcc cgtgacggtg tgggtttcca
     5701 gaaacgtctg tccatcatga tggcggagaa cggccagatg ccggccggaa ggggacagtt
     5761 gaatcgattc aattcgggga agcgaagcga aggtcgcggt gggtggcgac gtggagccgg
     5821 gctcggcgta gaccggtacc atgctccaga caagtagcag aacagtgaca agtccgtaga
     5881 tggccggcac ggtctatccc tacagttacc aggacatgcc tatccggggt catacccgac
     5941 gaatcctgtt atcacatgtt cgggagacgg ccgtccatcc cattttttac gcaaagggac
     6001 gcccccaaag cttcctgacg ccacactgaa gccatccccg cagccacgat gcggacgctc
     6061 acctgacgat gagccccgca tcgccaactt caccgcgact taaaatttat actccacgcc
     6121 gccgaacgca ctgatgccga atcccgggtt ggcgaaccga ttcagtgagt cgttgacgac
     6181 gaccgcaccg gcataggtct tgttcgtgag gttccggccc tcggcgaaca acgtccaatg
     6241 ctcatcgata ttccatcctg atcgcatgtt gatcagcgca taggaaggat tcttgatcgt
     6301 gttgttgtaa tccgtataga aaccggacaa cgaccattcg acgtttggcg cgatccacca
     6361 accggacggg tgatcgtacc gcgcttccac attcaggcta tgctccggcg cgccggccac
     6421 cgtgttgccg tccttggcaa tcaatacatt ggggccgccg attcctccac cccgcacatc
     6481 gtccgtgaac ttgaagcgtg accaggtgta ggccaggcgg gtttgcagac tgtcttcctt
     6541 gccggctccg ccttgcgcga agaggccttt cttcaggacc atggcgccgc ccgcttccac
     6601 gcccgtgtgc ctggtcccat tcgcattttg aaaggtgccg gtgttgttga tgttcgacgc
     6661 cagaatttcc ttctgcatct cgaggttgta caccgtcaca tcccagctat accgcttatc
     6721 tgctgaggta ccccggtgcc ccaactccaa ctgccatgcc cgctgcgcat ccagattcag
     6781 gaaaccggta ttcgcggaac cgttggcatt gagggctgag agcaactcta cgttgagcgg
     6841 cgcttcatac gaccggctgg cattgaaata gagctgcgag gtcggcgtcg tgcgatagac
     6901 gaatccgagc ttcggactga tcgccccgaa atgttgcaag ggcttctgcg tgctcgtcgg
     6961 cacagacggc gtcgccgggt tgaagggatt gcccgccggc ccgaagttat cgaccgtcgc
     7021 ctcacgcccc gtatagtccc atcgaccgcc gatcacgatc gtgaagtcct tcgtcgcgtc
     7081 gaacgcatct tcggcatacg cgccgaagta ggtggtcttc gcggtatagt tctgcgtcat
     7141 ggctccgatg ttgccattga tattgacgaa ccgctgctgc cgctgatgac cataccgtgg
     7201 ctggaatccg gcgacgaagg agttattttt cccgaacagc gaattggagt tcacataccg
     7261 gaattctccc ccgacgttgt tgttttcctg ccggatcgtc tggaagatcg gatggtcgac
     7321 gaattggttc gagaaatagg ggatgatttc gaaaaactga ttcggcgcga actcgttatg
     7381 gtacgcaatg ccgatccgtt gcagggtgta gtatcggccc cagttacaag cctggttgct
     7441 cgacacgcag gcaaaaaacg gcggcgcccc gggaggactc tgccctccgg tctgttgcct
     7501 gttgaaaaac aactgctgat tcgtcagtga accgggaatc cgctcggcca cattggcctg
     7561 gagaaagtag gcgcggattt cctggtggtt gccgagctgc aagccgatgt tggcattgat
     7621 gcgttcgcgg gcctgctgac tgttatcctg aaagccatcc tggcgattgc cggacacgct
     7681 gatgtagtag tccatcgtgg cgctcatgtt gcccaccttg aacggctgca acactttgcc
     7741 gctggacacc tgcccgctca ccatgccgaa actgccgccg agcatccgca tttgcagcgt
     7801 cgaggcgctg tagcccgtgc gcggcacgaa gttgatcgca ccaccgatgc tgttggctcc
     7861 ataccgcaac gcattcgccc ccttgtagac ttcgatgcgt tcgtacgcca gcaggtcgat
     7921 cgattcaaag tcggaaaatc cgtcggcgtc gccgaagaaa atgccgttga tcagaatatt
     7981 gatgccgcga tggtggaaat tattccgtaa ggaggtgccg cgaatttgaa actgcccttc
     8041 atcggcacca aaccgggatt gaaaccggac gcccggcgcg aactgcagca cgtcctgcaa
     8101 atttaacgcg cgcgattcgg taatctcctt ctcctggatg aggatgttgc tgccgggacg
     8161 gcgcgcaaac tcccgtttca catcctcgac gttctcgatg cgtttcccac tcacttccac
     8221 cggttcgacc gcgatcaccg gcgcctcctg ctcttgcgac tcttcagctg cgagccccgc
     8281 aggcggttgg ataccgcccg atagagccac tgccaagagt ccaatgacta tgatgccgac
     8341 acgatcactg cacttcattc cctcgcttcc gatctgccca gaccgtgaat gactaatgaa
     8401 caatgacgcg atgctggttg agcgcaccac gcactactga ctccgttgac ccgctgccga
     8461 ccaggcagcg tccttcataa gggcaccgtc caacggcacc ccctgtttga accttcgacg
     8521 aatggcctgc acctgatcga aatcttcgat gggattgcct gccaaagcga gaaaactggc
     8581 ctcatacccc tccgcaaact ttccgattcg acgcgcagga aaaatcgccg ccggtgtcgc
     8641 ctcgcaccac atcttgagga gcgtgcgatt gtcgaacaga tgcaaggtgc tcaggtgcag
     8701 cacctcggcc agcgacgtct ccgcatggtc gctgccgatc acgagtgtga ccccttcccg
     8761 ctgcagcaag tgaagattgt ccttcagcac atgagaagcg gcatcagcgg gcaacgcatt
     8821 gtgcttcatc ggctgcccgg tcccatgccc tgcatgagaa ccatgctggt gatgtccacc
     8881 gatggagggc atcgcctgtc ccgccacact ggtagtgacc actcgcactt tgtgatccgc
     8941 tgccaggcgc gcatcctcct ccgtcaaccg ggcacgttcc gcatcgtccg cacgttgaat
     9001 caaccaaccc ggcacatgcg ccagctcatc aactcccgca cgaatcgcct ggcggaaatc
     9061 agccgccgtt tcgacgtggg ccgtgacttg cagcccggcc gcatgtgccc tcgcgacaat
     9121 acccggcact aacgcaggat gcagcccttt cctgtgatga gcaggtttga ccttcgcttc
     9181 ctccccatcg tcctcggaat gcaccagata gactttgaga aaatcagggc taccgcgcag
     9241 gatcaccggc cacttggcct ccacctcagc atccgtctcg accactacat acgaccggtt
     9301 ttcaaaccat ccccgttcga tcggcccgac cgccggctca taccgcccga ttcgcaacac
     9361 atcttcatat aacgctgcgg gatgcccgcc atgtcccgta agcccgacat gcgcaaaggt
     9421 cgcatcgata ctcgttggca tgttgatggc gtgcttgatc tgctgcgcga agtcccgcac
     9481 gtcgttgggg ttcttcacgt aaaacactcc gtcgtgcaga tagcgctgcg cgaccgcctg
     9541 tacattccac ggcccttcga cgttgtgatt gtgcgcttct ccaaagggcg gaaccatgta
     9601 aagaccagcg agatccacgg tctgaaccag tcccgctgga ggccgcttcg tcaggcggcc
     9661 ttcgacggaa taccaggtgg ctgcccgaaa tccttccccg tcgaaccact ggccgttgat
     9721 gaaggcatac tgcgggagcg acgggggaac tggatcacct acgccccccg cacaacccga
     9781 tgccatggcg attagcacca acgcgagcag caggcgcacg aggctcgtcg cgcgtgcgct
     9841 gacgaccgac gatgacctga cggctattcg cgtcatacac tgagattcat ttcgtgcgtc
     9901 ttgttgcttt ggaccagccg atccagcgac gcacgaaaca cccgcagact gccgcgtcct
     9961 atcttggtgc cttccaaccg gccgtcttct acccagcgat aaatcgtcca ccggctcacc
    10021 gccaacacat ccgctgcttc tcccacacgc aagagttgct tgttcatcgc aatctccttt
    10081 gaagtagtga tgtgagcggc aaccatcgac gttgccgctc acgaccacag ggttagaaca
    10141 aaccgaacgt cacgccccca taaatcgcgc ggccgtagcc ggcaaagaac gcctgttgcc
    10201 cggcggcagc cccggcggtt tctccgccga tcacctgccc ggaagccgcg tatttcttgt
    10261 cggcgatgtt gtctacctcc acatagaact tcgcgaatcg gaaccaggaa ttcttgagat
    10321 cgaagttctt gtgcacattg acgtttgcga tcatgtagga cggaatgccg aacgtattgc
    10381 tgttgttcag gaagtaactg ttgtaataac tcccctcgac ccaggcgccc cagccagtgg
    10441 ccgtatggtc ataagccacc ttcgtattca gcacatcggt cggcacgttc ggcactttgt
    10501 tgccgtcgcg cgtcaggaag ccggcggcgg tgttcaaccg atcggcaaag ttgacgtact
    10561 tcgcctcgat atgggtatac gcaccggaca atcgcaaacc ctccatcggt cgccagtcgt
    10621 agaacgcttc gatgcccttg tactgtgagg agtcagcatt gactgaggcg gtgttggtac
    10681 ccgacaccgc ctgtgtgatg atttcgtcct tgaagaaggt ccagaacccg acgagttgca
    10741 cggtgagggt cttcgtcagt ttggactcgg tcccgatttc gaagttgaga ttcttctgcg
    10801 gcttcaggtc gaagttcgtt cccggctgcc cggtcaccgg atctctaagg agattggcaa
    10861 atcccggaat gccatacccg gtcgatgcgc gcacccaatg ccgttgagca tcagtcggct
    10921 tccacgtaac cgacatttcc ggcgcccaat tggtgtaact gcgatcagcg cccgcacggc
    10981 tgcttacggc accgcccgta tacccgatcg actggacgct cagctgagac tgctcaaatc
    11041 ccaatccggc cgcgagtgtc accttgggaa taaattccag ttcttcccgg aaacggccac
    11101 cgacgtttct gatcgtgccg cgtgaatttt ggaggagtgc gccgcgagtg ccgaagccgt
    11161 cggcgaggtt ctgaaacgtc tgcgcttcct gctccatgtt gctgagaaag aagccgacgt
    11221 aactccgtaa cggcatgtcg aacagtcgtc cgtcgtggcg caggtcggcg taatgcttat
    11281 agttcggatt cacgttgtcg ctgatttgag agaaggtttg attgatgtcc ttcacatcgt
    11341 aatcagcctc gatcgtcagc accgtattgg cgttgatctg acgctcgtag atacctccga
    11401 tgatggtacg tcgatcgagc cggcgctgcg ccagccgttc cgcatcgcta caggtgttgg
    11461 cattacacac cacgttggta ccaccggcct gccgatggtc ggcgttgaat tgagattgag
    11521 tcaaacgggt cggcactcgc gcatcgagcc agttcgtgat ggccttgaag tagaagttct
    11581 gcttgtcgtc gatcttgaac cgcaagttca ggttgatcgt ttgagtgttg taactgctgt
    11641 tccggatgaa tccgtcttcc cccacattac tgacgaacag cgacgcatcg agattttcga
    11701 attgctggcc aacggcaatg ccttccttgt ggtagccgaa ggagccgccg ctcaggaatg
    11761 tctcgactcc gttgatatcg ctcccccgcc tggttctaaa gtgcaccatc ccacccaggg
    11821 catagttgtc atacagcgat gacgaggcac cccgcgtcac ttccacgctc cgcatgaacc
    11881 aggggtcatg aatgtccaat cgcgacaagc cgtcagattg ggtttgaatg aatccatcct
    11941 cgtacatctt gatgtcacgg acggcgaacg tcgtcttggc cccttgccct cggatcatca
    12001 tgctgaaatc gcgcggaccg ttggcctgcc gcaataccac gccaggcagg gactccatcg
    12061 attccttcat cgttcgggtt ggctgggctt ccgtttccga ttgttcggtc gacgacatcg
    12121 acaaaccctc aggccttcgc tgcacacgat cgctcacgat actgacgtcg gacagctcgt
    12181 attgcggtat cggctcggcc gcgaccgatg cggacgattc ctcaactgcc gccggctggg
    12241 ccggttgaat cggcttggcc gctgcgccgc cggccggttg ctgctgcaac tcttccaact
    12301 cacgaagaat gttctgcagt tggtcacgca ggtcgcgctc gcgtgcgctt ttctccacaa
    12361 cggcgccagc tgccgtctct tcctgcgcaa ttgagggcac aacggtcact ggcactgaca
    12421 acaaggcaca acataaagcg gcatgaagtc tgaatgaatc acggcgggcc cacatacaga
    12481 tcctcccggc acaactgtgc acacgacaga agagacaatc ccctcgcgcc cctgtctgaa
    12541 cggacgagaa gagaagctag acgacctagc gctataaaaa agagttatga ctgaactggt
    12601 ggcgcgcgcg aggcggtgtg gaagcccacg cctcccgcga cgatggcatg gctgctctcg
    12661 acaacgagcg tcgccacgaa aaacggctgc agaaagagcg ccacagggcc ggcatccgat
    12721 gtcggattcg cctgacaggc ccaggcacag aaactggagt gagagaccgt accgccgtgg
    12781 tgatgggcgg ccggtttcgg atcggcatga tcgactacgc aggcgactga gaagacggcg
    12841 agaaccaggt agagtccagc cagcgcggcc gcaagtccaa tggaaggttt cgtcacacgc
    12901 atcatggcat caggctctgc aacgcgtgca cgacattagg cgcatcccag tcgcggggtc
    12961 cgacggcacg gccgatcaac atgccctgtg ggtcgataaa taccgtcgtc ggcaaggccc
    13021 gaacgaggta ggcttgggac accgcttgat cttcatcgaa taacacgggc acctgcacat
    13081 gaagattggc cagaaactgt ttaatgccct cgcgttggag gtcggtcgtg atcgtcatca
    13141 gcgcaaaacg ttcgggatcc agctgccggc gcaatcgctc gaaggccggc atctcttcct
    13201 tgcagggccc gcaccaggtg gcccagaagt tcaccacgac gacctttccc ttcaactgcg
    13261 cgaggtcgac agctgcgcca tcgagtgatg tgagctcgaa cggggccgcc acagtcccgg
    13321 gcgcaacccg tccgaccttc agcgccgcta gccggtcgtc ggctagtgca gggtcgcacc
    13381 agagtccgat cgccacaaac agggcaaagg tctgcacgaa tcccaaccgc atcacacgcg
    13441 ccgtcccatc tactggcctg ccgctgctgc cgccgtgacg gacggcatcc gcagggtctg
    13501 catgacaatt ttgtggcccg gcatcccatg ttccatccag gccatggcaa ataacccgtg
    13561 ccgattcacc gcgactaccg gtgtctggct ttttcgctcg ttcagcttct gcggcgcagt
    13621 gaacgttgcc ccgcgatcgg tggaggcact catgaccaca tctcgtttga ccggtccctg
    13681 ctcctcccag atcacaacca gcctgccttc aggatccacc gcgatctgcg gatggtcggg
    13741 aaaggtgttc ttcgacacat tcaactgctg cttcggcgaa aacgtctggc ctcgatcatc
    13801 ggagaaggcg agatagaccg ccggaatttc gtcggtccct tccgtatacc agacgatata
    13861 cagtcgtccc tgccagtcga cgcccatcga ggcgggccga tgcggacagg ctggaaagac
    13921 ccatttgtca tgtccgacga tgaccgatga gccgaacgtc tcaccatggt ctgtcgaacg
    13981 cgccacgacg gtttctcgca cgttcccctc aaaaattttc cgccaggcca catacaccgt
    14041 tccgtcaggt ccgctcgtga tggcggttcg gcaacagaca caggtcccct cgtccacctt
    14101 ggcatttttc gtgatcgtct gcccctgatc cagcgaccgc gcgacatagg tgccgggatc
    14161 ttttttgcct tcacgtccat cgatccagga cacatgcaac cgtccctccg aatcgcgatg
    14221 gagcgcatcg aaggtatgtt ggatgacacc cggatcgtca ttcactacga tcgacggctg
    14281 gaaagtccgg ccgccgtcct tcgaatggct caggcgcaat tcggtcgcga acggttgctc
    14341 gggcgtagcc ttgggatggg ccaggcccca ggtcacgaac acgtcttccc cctgcaccac
    14401 gagcgccggc gcttcctgtc tccagtacgg gatgtcctcc acgcggttga tgcgcactgg
    14461 ggcacccatc ggtcctccag gctcgagact ccgggcatac atgacagacc gcacctcttt
    14521 gttttcttcc atccaggcca gcgacaaggc ccccgcctcg tcgatctgca ccgacgggcc
    14581 cacgaagctt ttcaccttat gttcggtggt gaccttggga ccaaactgca tacccgctgg
    14641 agactccgcc aatgccgtgg gcatcgtggg tagcaatccg aataacagcc cggcacacaa
    14701 ggcttgtttt gctcgtctcg gcatgagact cgtccgtaga ccgcgcatac tttggagcat
    14761 tgttttaccc ccgtcggcta ctgaagctag accgtacact gcgtcaaact cctcacgctc
    14821 caggcgtgaa caattgaaaa tacttggtca cgccaaatgt gtaactggtg gcctgcgcaa
    14881 ggttgttgtt ggcatcccga tagagaggca cctgggcata aaaataggcc tgccatgtgt
    14941 ccccgatatt gaacagcacg cccggcgcag caccgaacca ggaagagccg gtgttgggga
    15001 tcccgcggtc caggatcggc ccggaaccga tgctcgcgct cagactgtcg tgcgtcatat
    15061 agcggtagtt cacctgggcc gtgaatacca gccatggcaa cgtcaccaaa ttgaacccgc
    15121 cattgacaat gtattcgtcg ccgaactgat acccatcgtt gttcttgaaa gtatgtcgat
    15181 agctggcgaa cacgaattga ttcaaccgat gcggtaggat ctcatacgat tggtacatcg
    15241 tgcccacgag cccgaattgt ccgcgcccca attgtcctgc cggttccaag actccgccca
    15301 gattgtcggt ctgccggtaa tcgccggtcg gcagctccgt accaaccccg aagaccacca
    15361 tatttttcaa tgaagcgaga acattgtatt tcgccatcac tcgaatatcg ccaagcccct
    15421 tgtccgaaaa gcggctcaga tcttcatttt gtccctgatg gaaatggtag tgctcatgcc
    15481 ggcgggtcag atagggcaac gtgacctgca atcccaggcg atcggtcacg ccgtaattaa
    15541 aatccagcgc gaaggagttc gtataggttg agcgttcatt atgatggttg ggaatgatgc
    15601 gccgcccgat ctggtccgct tcggcaatca ggcccgtcga tccgctgggc aggctcattg
    15661 gcgtataact gtagataaaa ttcatggtca gcaggccgga ttgcgggacc tgctgctgag
    15721 atccaattgt cacgaagcaa ctgacggcac cgcaggcggc ataggcctga ggtatggacg
    15781 tcacactcac cacagccacc gcgacgattc cgagtaccga caccaaaaac cttctactgg
    15841 gagcccacat gacacacgat cctttctcca atcacggttg atgatacagc tcgcgcctgg
    15901 tgccggatgg aaccccggct cagacgctac gggtgatacc gaaacgggat gaatcagagg
    15961 gaaacgattc aggccggaga agaagacggc gggcctcgcg acggccacag ggtgcgggat
    16021 tcgatcgaga cggaatcgac gacgatctca tcaagcaagg caatgacaga gatcgtgacg
    16081 ggggccacct caggcacggg ctcgagatct tgccccgctg cacacatcca ggaacacatc
    16141 acggtcccat ggacaccggc ctcatgatgc gcatgttgcg ccacatgctc cagcatggag
    16201 ggatacgcaa tgccctgcag cgccagaaag gcgcagacca gcaggagcgg catcagggta
    16261 tgtcgtacgc gagtcctcat tgggtcaatg caacgccgcc gccttaatgg gcgcagcctg
    16321 ttgaattcga ctgacgatct cctggatctt ttgctcggtc aacaacccgc ccttgatgta
    16381 ttcctgcacc acgccctgct ggtcgataaa cacgctcaca ggcagaccaa acaccccgta
    16441 ctgattggcc acccggtttt cacgatcgag caacacggga aacgtatgtt tgtactgctg
    16501 aatgtgttca cgcaccttgg cttcatcctc taattcattg accgccagca ccacgaaatc
    16561 cttatcacga agctggtcat agaccgtctg catcgccggc atttcggacg tgcagggctt
    16621 gcaccaggtg gcccaaaaat tcaccaagac gatctttcct ctatactgct ccagggtgtg
    16681 cgccttgccc tgtaaatccg tcagcgaaaa cccgctggcc ggcatccccg ccgctggagg
    16741 cctcgacccc atcgccagtg ccgagccgac taacgtcgag acgagcaggg ccgtcactat
    16801 tacattccgc gtaaggctct gtatcatgat tgccgatctc cgacaggctc ctgattgagt
    16861 gttcatgttg ccttctcccc aatcacctta ccggttcaaa ctgtatacga ggggcagatt
    16921 cagcacgacc atcggcgcgg tgagctcatg cttcatgtgc aatgggcagg cccgtcgcac
    16981 cgcttccatg gccgcttggt ccagcgattc atgtcctgaa ctcttgacca cttcgacact
    17041 tttcagctgc ccgtcattgc tgatagacac cttcaacacg accttcccct cccagccgtt
    17101 taagcgcgca gtgctgggat aatgtcgcag ttcgatgacg cgccgtttca aagagtcggc
    17161 gagccagccg taatcggcct tgggagcagg tcgcgcaacg gctgcctggg ccaccacttg
    17221 atgttcacgc aacgtcgagg ggtccggctc agtcaccgct gccaccggcg cagtcggcac
    17281 cacgaccgcc tcaacgggag caggaggctc ggcgaccggt tcactgacag ctgctgcaac
    17341 cgattccgcg gcaggggcgg gatcgaccgg ggccgcggtc gccggcaccg cttcgactgc
    17401 gggaggggtg tgcaccaccg gtgcgctggc cgtcatcacc ggctcaggga ccgtttcgac
    17461 caccgctggt gcgctgacaa cctgctgcgc ctgataggtc tgggccgtgg caggcgccgc
    17521 ctccatgacc tgctgctcca ctaccgccgc cacttcacgc tgcatttcgg ccggagccac
    17581 cgcttgcacc accggttcgg tcttgtggac ttcctgcggc tcaaccggct tcgcttgcat
    17641 ctgcacgact tcctgcttcg actggatctg ttcctgcgga cgcaccgttt caaccacggg
    17701 ctggacttca cgctgcacgg cttgcgggag cactctggtt tccacctgcc gttgcaccgg
    17761 ctgaggtggt ggctcgaccg cacgaaccgg ctgggatcgc ggcttcaccg gggtcggggt
    17821 gggcggctgg acccggggcg caggagttgg gccttccagg cgcgccactt cacgcggcgg
    17881 ctcgacaagg gccacgtccc acttgaacgg ctctttctcc accatgacgg tcatcttcgg
    17941 caggagaccc aacgcgacca gcgcaaggca ggcgtgaaag accagcgacg ccccccatcc
    18001 tgcgaccgat tggtttgagt gccccgagtg cgcgacgatg acattgtcca tgggaacgac
    18061 ccctttcgac acgatacgtt ccgacgttac ttgaagacca ctttagagag cgaaccgatc
    18121 gggacttcaa cctggccgaa atcggactcg cccttgaaac tgccgctcac cgcgggaaca
    18181 aattccccgc tcttcccgtt ggccaaggtc acggtcatag ccggtgcagt cttgtcggca
    18241 cccggcttca actcgatctg tttgatctga tcgaacttga tattgaccgt cgccgtgccg
    18301 cgcttggatg gcaggtgccg cagctcatgc gggacgaacg acgtctcact cattttttct
    18361 tcccaataga aaatgccgtt cttcagctca gtctcgacac cctgagcatc ggtcaccgca
    18421 atggtgaatg ccttttcggc cttcgtgtcg gcagcggaga ccatgggagt cgacaccgac
    18481 aacaccagcc ctgtaagacc gcatacgccc agacggaaca accactgatt cacctgcatc
    18541 ttcatcactc cttcacgtct tcgcagagga attgacgcca caccattcct aacgattcac
    18601 gggatccagc ctccaggcga agcgacggaa cagacaccat cgcgaagcga ccatcgaaag
    18661 gaggaatgca tccacagcat gcgagagacc tcgctcaact gtttgatccg ggctccccga
    18721 cgaccagtcg cggagcgaga ctatgcacaa gggagacgtg gaattagaac gacaggggtg
    18781 gagcgcgcga ctgacagagg gcagagacct gagggagatg gacgatatcg gaaaggaacg
    18841 aggcatcgcc ggagagcgcc aagacatgcg tcgcatgaac cggcgtgacc acatgtacac
    18901 cggccgtggc gcacatccag gcacagatgc ctgacccgtg cgttccggca tcgtggtgcg
    18961 cggcgtggtg cagctcatgg gcgaccgcgc tgccggtcgt gaccgccagg agggctacga
    19021 cggcaaagac gacaacccag acgaggctat gaaagcggga acttcgcata gtaaagaacc
    19081 acaacctggc gcgcatgcta gcaaactcgc cgagggagcg tcaacgcaac ttttcaccct
    19141 gtgcggggtt gacgactagg gcccgccgac accaatgatc cgctcaacgc gctcatcacg
    19201 tccgccaagg tcacgatacc ggcggtactg cgccagggca cgccccatgt ctttcctgtg
    19261 gagttcgtaa aacaccccga gattgtagtg ggcttccgcc gaggtgggct tgagcgccac
    19321 ggcccgttca tgctcccgct cagcctcatc cagccggttc aagctggtat agaccatccc
    19381 caggttcaag tgggcttcgg catactcggg gcgataccgg atcacctcca aaaactctcg
    19441 ctcggcttcg accagcaatc cctggctccg atagacgaat ccaagattgt aatgggcatc
    19501 caccagatcg gccttgaccg caatggcttc cttgaacgcg gcggcggcag cattcaggtc
    19561 attgctccgt tcggccagac gccccaggag ataccaggcg ttggcatgtc cgggattggc
    19621 ctgcgtgagg cgggacaagt attcgcgggc ttgcttgaga tcgccttgac tgtcatagag
    19681 actcgccagg tgatagagcg cctccgcgtg atccggtcgg atgcgaagga gcgattggta
    19741 cattttcacg gcattcgtgc gatcatgccg ttcctcgtag agccgcgcta aatccaggtg
    19801 cgcttcttca ttgccgggcg cgatatccaa cacctgccgc agcacctgct ccgcgtcagt
    19861 ccctcgccct tgcgccagat agacatcggc gagatcgttg agaatctcag ccgacggtgc
    19921 gctcaatttc agcgattgtt tgtaggcttg aatcgcttca tcgggattgc gtttgctctg
    19981 aaaatagatc aatcccagga cgtggtacgc ctccgccagt ttgggatttt gcgcgagcgc
    20041 tttcttcagg gcttcgatgg cgccgtcggc attgccttca cgaaacagaa ccacgccgcg
    20101 ctcgtaactg cgacgtgccg attccggaga ggcacttgag gcggctgaag attcaggcag
    20161 agaggtgacg aacagggcca cgcacaagag tggaagagtc gtcagaagat gacgtgaacc
    20221 ggtgacccgt tgccgcatcg aaatccgcaa tggaatccgt gggtgacttc gccgtaagag
    20281 ccggcgagat ggtgggccca ctatagatgt caccgcatcc gaaagcaacg acgagcacct
    20341 gtcgcagtcg ttcatcgtga aacagttgag accgtcgccc ctgtttcggt aggattcgat
    20401 gacggattcc tgtatactcc gccgccgagc ccgtgccaga ggggcgagct tcgtctcccg
    20461 gcaggttcga gagaataaag gtacgtctgc atgctgaccc aagttctgaa catcatattc
    20521 ggcagtaaaa acgatcgtga gatcaaggca ttgcgcccga ttgtcgagca gatcaacggg
    20581 ctggaatcaa gcctcacgcc cctgtccgat caagccctgg cggagaaaac gcaggaattc
    20641 aagaagcgtc tccaggacgg tgagaccctt gaggacattc tgcccgaggc gtttgccgtc
    20701 tgccgcgaaa tgtctcgccg ccgtttgaac atgcgccact tcgacgtgca gttgatcggc
    20761 ggcatgattc tgaacaaagg ccggatcgcc gaaatgaaga ccggtgaagg taaaaccctg
    20821 gtcgccaccc tccccctgta tctcaatgcg ctggagggga agggcgccca cctggtcacg
    20881 gtcaacgact acctggcaaa acgcgacgcc tcctggatgg cccagctcta tcatgcgctc
    20941 ggcctgacgg tcggcatcat ccagcacgat gcctcttttc tttacgatcc gacctacgag
    21001 gcggcggaca aacgcctcca gcacctccgc ccctgcagcc ggcatgaagc ctatcgcgcc
    21061 gagatcacct acggcaccaa taacgaatac ggcttcgatt acctccgcga caatttgatc
    21121 gtcagcgact tgagccagtg cgtccagcgc ccgctgcact tcgccatcgt ggacgaagtc
    21181 gacagcattc tgatcgacga ggcgcgcacg cccttgatca tttccggccc gacggatcaa
    21241 accacggatt tgtactaccg catcaattcg atcattccgc agctcaaacc ggagcacgac
    21301 tacacgatcg aagaaaaaac caagaccgcc tcgctcacgg aagagggcaa cgtccgcgtg
    21361 gagaaactgc taggcgtgga caacctgtac gacctgcagc acatggatct ggtacaccac
    21421 gtcgtcaagg ccctccaggc ctatgccctc tacaagcgtg acgtggacta tgtggtcaag
    21481 gatggcgaag tcatcatcgt cgacgaattt accggacgct tgatgcccgg ccgccggtgg
    21541 agcgacgggc tgcaccaggc cgtggaagcc aaagaaggcg tcaagatcgc caacgagaac
    21601 caaaccctgg cctcggtcac cttccagaat tatttccgga tgtacaaaaa actggcgggc
    21661 atgaccggca ccgccgatac ggaagcgggc gagtttgcca agatctacaa tctggacgtc
    21721 aacgtggtcc cgaccaaccg gtccatgatc cgcaaagact acgccgacgt cgtcttccgc
    21781 accgaaaaag aaaagttcac agccatcgtc gaagaaatca aagactgcca cgagcgcgga
    21841 caaccagtcc tcgtcggcac catttccatc gagaaatctg agcgtctggc gggctacttg
    21901 agccgcaatg gcatcaagca caacgtgttg aacgccaagt ttcacgagaa agaagcggaa
    21961 atcatcgccc aggccggtcg caaaggcgcc gtgaccatcg ccaccaatat ggccggccgc
    22021 ggaacggaca ttctcctcgg cggtaatgct gacttcctct tcaagcgcat tctctatcaa
    22081 gacgacacgc tgacagacgc gcgaaagcag gaaattttcg acgaaatcaa agccgattgc
    22141 gagaaggaca agcaggccgt ggtcgcggcc ggaggtctcc atatcctcgg caccgagcgg
    22201 cacgagagcc ggcgcatcga caatcagctt cgtggtcggg ccggccgcca aggcgatccc
    22261 ggatcttcac gcttctacct gtcgctcgaa gacgatctca tgcggatctt cgcgtccgaa
    22321 cgcgtctcgc agatgatgct caagctcggg atggaggaag gtgtcccgat cgaacatggc
    22381 atggtcaccc gcgccatcgc gaacgcgcag aaaaaagtcg aggcgcacaa cttcgaagtc
    22441 cgcaagcagc tccttgagta cgacgatgtc atgaacaagc agcgggaagt gatctaccag
    22501 caccggcatg ccgtcctggc gggggaacac atccagcagg acattcacga catgatgaag
    22561 gatctggtga atgccttcgt cgagacctac tgtcccgccg atcaatacca ggaagagtgg
    22621 gactacaacg gtctcggaga agcgctgcag ggccagttcg ccctcgacat cacccagggc
    22681 aagggcagtg tcgccgatca ctttaaggac gtcggccggg atgcgatgat cgaggagatt
    22741 cagacccagg tgcggcgagc ctatgaccac aaagaacagg aactgggctc tgaactcatg
    22801 cggtatctcg aaaaaatgct cctgctccaa gtgatcgacc atcactggaa ggatcacctg
    22861 ctgggcatgg accatttgcg ggatggcatc ggcctgcgcg ggtatggcca gaaagatccg
    22921 ttgatcgagt acaagcgcga agggttcgac atgttctcct ccatgatgga gcgcatcaag
    22981 tccgatgtgc tggagcgcat gttccgcgtg caagccgtgc ggggcgaaca accgcctcct
    23041 ccggcacccg aaccgatccc tccgccgcgc atggtcctga accgcagcga cgaaccggcc
    23101 actcagaccg tccaaagcca gggcgacaag accggccgca acgatccctg cccgtgcgga
    23161 agcggaaaaa aattcaagaa gtgtcacgga gcatagccgg gaattcctgg cccgcctccg
    23221 cggagtgaac agaaacaaac ggtccgcaaa tgtcgagaga ctcgcgtctt tccgggtctt
    23281 ttcgccttga cttgccgctg agccagcggc taatctaacc ccctcttttc cctagagaat
    23341 ccggtagaag agcggtttct tgcgatggcg cctgaaacgg cagtcgacta ccgacaaggg
    23401 tggtgtcact ttgagcactg ggcatccaca actcctggcg gaaatcaggg ctgaaatcgc
    23461 agcgtcggga cctattccct tcgcccgttt catggagctg gccctgtatc atccgcaata
    23521 cggctattac gttcgtcctg tcgaagatcc gacccaggaa cgtattggat ggtccggtga
    23581 tttctatacg agttccgatg tgcatccgat tcttggccag gcattggcca aacaggcgca
    23641 gcaactcgat gcctgcctgg gacatccaaa cccgttcacc gtggtggaga tggggccggg
    23701 caaaggattg ttggctcggg attttctcac cgcctgccag aacgcgcccg gcaatctcag
    23761 cgaacgactc cgttacatcc tgattgaacg cagcgcctca atgcgcgccc tccagcatca
    23821 gcatctcgcg ccgtggatcg gccgagccgg tcacgtgacc tggcttgatc gcctggaaga
    23881 tcttccgccg gacagtgtca ccggcctctt cttttccaac gaattggtgg atgctttccc
    23941 ggtgcatcgc ctggcggtgg tggatggcca tccgcaagag atttatgtcg atgttcacga
    24001 agagggcttc acggaaatcc atcgtcccct ttcggaagag ctgtcggcct atttgcaaaa
    24061 cggtggcatc cggctgcccg atggataccg aaccgagatc aatctcaagg ccgttcaatg
    24121 gatcaaacag gtcgcgcaaa tcatggcacg cggcctggtg atgacgatcg actacgggca
    24181 cacggccgaa gacctctacg gaccggagcg caagaacggc acatttctct gctactacag
    24241 tcagaccacc tcggaagacg cgtacgagcg ggtcggccag caggacatga cggcgcatgt
    24301 ggacttcacc accctggcgc aagcaggcca ggcggccgga cttgagttga ccggctttac
    24361 gaatcaaatg agttttctga tcggcctggg cgccgaacag ttcctggagt cgctgcagcc
    24421 ggaatcggcg gagttttatg ccgccatcca cctcctgcga ccggaaggaa tggggcggac
    24481 gttcaaagtg ctggtgcaac atacgggcat ggagaagccc gaactggacg ggttgagatt
    24541 caagccgttc ttcggatcga ttttggcggc tcgttccgcc gcgtaaagga accggcagga
    24601 cgccatctaa ttgtgacgac cgccgtgaga gggttttcgt ttcacgattc ccggacaaac
    24661 catgggtaac gaagccgcac cgctgaatta catcccgatc ctgattttta tcctgatcgc
    24721 ctttgcgttc ggcgctgtgc agattctgtt gggacgtatc gtccggccga gccggcctta
    24781 tcgcgccaag ctcgcgccgt atgaaagcgg cagcccgttg ttctcggacg cgaaaataca
    24841 atttccgatt cgctattaca tcatcgcgat gctgttcgtg atctttgata tcgaaatcgt
    24901 gttcatgttt ccctgggccg tggcattcaa gaagttgggg ttggtagggt tagtggagat
    24961 ggtggtgttt atcggcatcc tgatcgtcgg gttctggtat gcctggaaaa agggcgcgct
    25021 ggagtgggac tgactggcgt acgacgtgac gcggcacacg tgagaaatgc aggccaatag
    25081 attcctgcct tccaattcac gaatcaccgt tcacgtttca cggggttgat atgagctttt
    25141 tagagcggca gctggatgca aatattctga cgacgaatct cgatgc
//
LOCUS       DAOQDS010000056        25063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_717_length_25063_cov_20.470409, whole genome shotgun sequence.
ACCESSION   DAOQDS010000056 DAOQDS010000000
VERSION     DAOQDS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 25063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25063
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_717_length_25063_cov_20.470409"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..988
                     /locus_tag="PK589_13660"
     CDS             347..988
                     /locus_tag="PK589_13660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ20702.1"
                     /translation="MQRAVIADDHHLYRRGTRDVIQEVFAPEVIAEANTGTQLLNLVK
                     ACNWDLCIMDVSMPVKSGPDLVEEVLQIRPSMPILVLSMHPEEHYAVRMIRSGARGYL
                     NKSTTDSALLTEAIRSLLAGHKFITSTVAECLADTVCAGSHPSSALHQVLSNRELQIF
                     QMLAAGTPIKEIASTLFISAATVSTYRTRVLTKLALDSNADLVRYALEHSLIA"
     gene            complement(1452..>1769)
                     /locus_tag="PK589_13665"
     CDS             complement(1452..>1769)
                     /locus_tag="PK589_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20703.1"
                     /translation="QAIEDGEYGKAYSYLSLRAQEQVSYDEFVTLIQENSVVMEPRGS
                     TFLSRKVNGNTASINGFVEGYSEYVSDVRYLFVKEAHQWKIVRFEWGVPRPMKEDQSN
                     ASG"
     assembly_gap    1770..1869
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1870..2206)
                     /locus_tag="PK589_13670"
     CDS             complement(<1870..2206)
                     /locus_tag="PK589_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20704.1"
                     /translation="MLEKLKFFGHAPKCEAPIRATLLYHYQGAVPQGPNHIVPPPVEI
                     LDAPSRVQTAQATRIHLPGILVFIFVVGMLVLGYWWGQTVKPVGIIRAHLQAIEDGEY
                     GKAYSYLSLR"
     gene            complement(2456..2776)
                     /locus_tag="PK589_13675"
     CDS             complement(2456..2776)
                     /locus_tag="PK589_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20705.1"
                     /translation="MSGRTITLRRVIWSLLAIWLFFGGVSFTEQVNSLLDTSDQDEQA
                     LSQLSLTLKPEIPPLPKQVTSSVVPIVLIPSVIHLADSSPSLSIPSQLLTLRPHQRVS
                     VYRI"
     gene            complement(2902..3348)
                     /locus_tag="PK589_13680"
     CDS             complement(2902..3348)
                     /locus_tag="PK589_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20706.1"
                     /translation="MSRAVSLGDPSAVRRPEKQELEVITMAWYHERIAEEKLTVLIRY
                     LVHSFPLASILSRQVGDRYHVFIIVPFDGSPEKTLQVETTVFRNRSLRVEGFTALLST
                     LNLRRLFDQNDRYDLNWFGRQPKARHAATFYGASLPQASQISAALA"
     gene            complement(3704..3779)
                     /locus_tag="PK589_13685"
     tRNA            complement(3704..3779)
                     /locus_tag="PK589_13685"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3744..3746),aa:Ala,seq:cgc)
     gene            complement(3857..4528)
                     /gene="purN"
                     /locus_tag="PK589_13690"
     CDS             complement(3857..4528)
                     /gene="purN"
                     /locus_tag="PK589_13690"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNJ20707.1"
                     /translation="MSAKAPRLVRLGVLASGRGSNLQAIIDAIEQHALSAEIAVVLSN
                     KQEAVALERARKHGAPAVWLDPKPFAGRPDSREAYDRAVQEVLQKHEVDLVLLAGYMK
                     IVTAVLVTAYQNRMMNIHPSLLPSFPGLDVQKKAIEHGCKIAGCTVHFVTEGVDEGPI
                     IIQAAVPIAEGDTPETLAARILEHEHRIYPRAIQLYAEGRLRVEGRRVFVAEPESPVT
                     GFHYP"
     gene            complement(4518..5555)
                     /gene="purM"
                     /locus_tag="PK589_13695"
     CDS             complement(4518..5555)
                     /gene="purM"
                     /locus_tag="PK589_13695"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNJ20708.1"
                     /translation="MTTYRDAGVDIDAGDEFVERIKPHVRATFRPEVMTDLGGFGGLF
                     RFQANRYQDPVLVSGTDGVGTKLKIAFLMDKHDTVGIDLVAMCVNDIAVSGAEPLFFL
                     DYFATGKLSIATAAAVVKGISDGCRQAGCALIGGETAEMPSFYGEGEYDLAGFAVGVV
                     DRPKIIDGKQIVPGDVVIGLASSGVHSNGFSLVRKVLLERSGLTVTTAMPELGGTLGE
                     TLLTPTRIYAKQVLSLMESCPIKGIAHITGGGITDNLPRVFPARCGARIRRGSWPVLP
                     IFEAIRERGSVAVDEMYRVFNMGIGLILVVAPDHADRVMAKARELGEQAYHIGEMVAQ
                     QTEDGVVEYVG"
     gene            complement(5775..7175)
                     /locus_tag="PK589_13700"
     CDS             complement(5775..7175)
                     /locus_tag="PK589_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ20709.1"
                     /translation="MSASILIVDDEVSILNSLSSILEDEGYDVSVAKSGMEALKHCAI
                     SPPELMMLDIWMPEMDGLETLKRLRELVPSTQVMMMSGHGSIETAVKAIKLGAYDYIE
                     KPLSLENVTLRVKHALDQHRLEQENRNLRTKVERKFELIGQAPVMQQLKQLIETAGPT
                     NSRVLIGGENGTGKELVARAIHQHSHRATRPFVAVNCAAIPETLIESELFGHERGAFS
                     GATTMKRGQFEQADGGTLFLDEIADMSLSTQAKVLRALQEQQFTRVGGTKLIKVDVRV
                     LAASNKDLLQEIEKGTFREDLFYRLNVVPIVVPTLRDRREDIPLLVKHFLRVHAEEQG
                     LKIKQVSPEAMDVFMQYEWPGNIRELRNLIERLMIMVPGPVIEAAHASVALQVRPQMS
                     AAQSAAGAQPVNTLLTRPYDSLRDARNAFEKEFIARKLREHHWNISRTAEDLKIERSH
                     LHRKIKLLDVEMRPEM"
     gene            complement(7224..9527)
                     /locus_tag="PK589_13705"
     CDS             complement(7224..9527)
                     /locus_tag="PK589_13705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ20710.1"
                     /translation="MPMPESTDMSKLIPPGVLREKEPSSPASIESERRKRHVRPVWIV
                     LILLLPCLALTFYYAQMAVPVGEESDSFLPSTGYAFVLLLVNLDLIGFVVLTLLLSRN
                     LIKAYFERRHRLVGSGFRAKLVAAFIGFSLIPTVLLALVASGLVNKAVDVWFNDQIEH
                     VMKDSYEVARMHHAGHVSLAINSARAISHEIFREELLLPEQRDLLVAAIARKRDEYAT
                     AGIEVFSAKMETLTKALDPEVPVTVLDLPIGQLVLQVINGKQELTSVQEAQTGRLVRA
                     GVPIASSVRRGEIDGVVVVDAYVPESLLGKMESIGRQYAEYKQMKAMKNPIKAGAYLL
                     VAVITVMILFSATWFGFYVARGITVPIQRLAEATEAIAQGDLSVRIEAKATDEIGTLV
                     ESFNRMTADLQGSKSKVEEANVSLRQSNLELDRRRAYIETVVDTIAAGVLSIDRQGIV
                     TTFNPSAERMLGLWADRFRGRSANEVFKEYQLDLFQSVYDRMLADQRDNIALEGQLDL
                     QGRFLTIGVHCSRMKDESNKDLGFVLIFEDLSELIKAQKAAAWREVAQRIAHEIKNPL
                     TPIQLSAQRLRKKFQDKAPDFDRICDESTQVIVNEVGSLKQMLDEFSKFARMPAPHMT
                     MNSLDDVVKEVVALYESAHREIICVVSLDADLPPFNFDREQIKRVLVNLCDNGIHAMN
                     HKGRLWITTRYDTKQRRAVVTVADEGTGIAPEDQDKLFVPYFSRKKTGTGLGLAIVRR
                     IVTDHDGQIHAGNHQPKGALFTFELPV"
     gene            complement(9725..10144)
                     /locus_tag="PK589_13710"
     CDS             complement(9725..10144)
                     /locus_tag="PK589_13710"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HNJ20711.1"
                     /translation="MGDEKDQGFVIRDRRGRGEEAPAAASAPTPPAPEPAAAESHHAD
                     AHGHAGHLPVNFSSFVISMGSSALMLMGEQLDPQQPAMPLNLPQAKEIIDLLSMLEEK
                     TRGNLTPDEQVVMKDMLYALRMKFVSLTSGKSSIHTP"
     gene            10280..11176
                     /gene="mazG"
                     /locus_tag="PK589_13715"
     CDS             10280..11176
                     /gene="mazG"
                     /locus_tag="PK589_13715"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNJ20712.1"
                     /translation="MSERFDKVVAIMARLRAPGGCPWDRKQTHESLKPYLIEETYEAL
                     DTIDRRDFPKLKEELGDVLLQVLFHSQIGTEAGTFTMDDVLQELGDKLVRRHPHVFGE
                     NASGTPGLNSDQVVHRWEDIKRAERKNAGKPDSVLQDIPQALPALLRAYQTQVRAARV
                     GFDWPDSPQGLADVLGKVEEEIGELRKAVSDAGAMSQPPSEPTADMAAELGDLLFSLV
                     NLARHIKVNPEESLRQATNRFTARFQHIEHEAARSGRSVHDLTLAEMDHYWNAAKQLE
                     SADRPAAASPLASKGEASQTSL"
     gene            11173..11787
                     /locus_tag="PK589_13720"
     CDS             11173..11787
                     /locus_tag="PK589_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20713.1"
                     /translation="MSSEQDPYEEGKRAGLHPLLVLFGAILVMWLLINLAVPSGKQKQ
                     AAGPEISQVGTEDPDAAPVMYKVQTTLTEFNAISILVPPQSTMSQVVGLLKQFKQARL
                     NQTLAGMLPATSPGHKLGDQAVADLYIFSDPKYASPEVVGTLARGAHAPGNLYPQAIP
                     FEEAMEQVRGHYRIDLNDTSNPDTGSIGFADDSGVHSKQYRRIF"
     gene            12088..12972
                     /locus_tag="PK589_13725"
     CDS             12088..12972
                     /locus_tag="PK589_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HNJ20714.1"
                     /translation="MAKNLLTQPYLTDELKAQDFLIKMRWPEGITCVHCQGEKVYTLN
                     VRGSKRRVLKCGKCRKQFSPTVGTIFEDSHIPLTKWFMTIHLMCSSKKGISAHQLHRM
                     LGVTYKTAWFMAHRIRHAMTQLMGATPLSGIVEADETYVGGKRIGAGMGYRDHKVPVF
                     ALVQRGGEVRSMVMPRVTANTLRAAIRTNVKPTTHVMTDGATVYTNVSNDVASHETVN
                     HYEKEYVRGNVHTNTIEGYFSLLKRGITGTYHHVAEKHLHRYLAEFDFRYNARKVEDS
                     VRTFSAIQGIEGKRLQYR"
     gene            complement(13431..13808)
                     /locus_tag="PK589_13730"
     CDS             complement(13431..13808)
                     /locus_tag="PK589_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20715.1"
                     /translation="MEQTQSIEELSATVKALQERVTALDYDLRSWQMKAHKYCPRCGG
                     PSVPSKPMNLPFSSLPLTDAVMMVVSEKDAPIGIRKLRAILEEKGMKEKMGRYGNNMR
                     TAITRLVKAGRLSREGDTVEMLK"
     gene            complement(14184..14600)
                     /locus_tag="PK589_13735"
     CDS             complement(14184..14600)
                     /locus_tag="PK589_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20716.1"
                     /translation="MLNDQLVISSVAGPFREPREPVFSYDYSIQRATWAATHAVRVKI
                     ALAEELDYVKTKLLGTVTGSPGQQLMLNKLLSRKIGDQKLRIAEEEGWLKERADVLVP
                     PFTGPLAHHFAQLDSWVQNQQETLRSEIKQTIGLAV"
     gene            complement(14629..16098)
                     /gene="hemG"
                     /locus_tag="PK589_13740"
     CDS             complement(14629..16098)
                     /gene="hemG"
                     /locus_tag="PK589_13740"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HNJ20717.1"
                     /translation="MARTSRSVVIIGGGISGLSTAFALLEQAAAADVPLSCTILDAAP
                     VWGGKIVTHRVGRLVMEAGPDSFLSQKPWAMDLCRRLEIADELINTNPVEKKASVLRG
                     GQLHELPEGLVTFTPTQLGPFFRSGLLSWVDLARMGCDVLIPPRRSTEDESLASFFRR
                     RFGQHACERVMEPLMAGIYAGDAEQMSLRATFPRFYELEQTHGSVIRGMMAARRARAQ
                     KASDGRPRHTMFVTLKNGLADLVAGLTTRIRETGGVLKSGVQAEALRVRSHQAGRWMY
                     DVICTDGTALSAEALVLATPAYVSADLVRPLSPLAAGLMETIPYASTATISLIYPAAA
                     VGTRLQGFGFVVPRVEGRDLIAATWTSIKWPHRAPPEEVSVRCYLGGVGREAILQQDD
                     EALVRCVREELASIVGLQATPHYVEVNRWHRAMPQYTLGHLDRLTQLDAALSRFGGLA
                     VTGAGYRGVGLPDCIRDGAEAAAQTLRYLQTASKREAES"
     gene            complement(16110..17054)
                     /gene="hemH"
                     /locus_tag="PK589_13745"
     CDS             complement(16110..17054)
                     /gene="hemH"
                     /locus_tag="PK589_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNJ20718.1"
                     /translation="MSAAPRPIALLLMAMGGPDSLENVEPYLLDVRGGRPTPPELVEE
                     IRTRYRVTGGKSPVLDITCEVAAALEQRLNASGERRFRCYVGLRHWHPFIRDTYAELL
                     KADPDHIVGLCMAPQYSSLSIGAYVKKVEEARADLADETPISYVTSWHRHPLLIAAIV
                     AHIRHTLERFPVEVRAQVSVLFTAHSLPERVVAMKDSYPEEVGATVQAVCAQLGGQPT
                     RFAYQSQGRSGEKWLGPSVEETLEDLAQAGHHHVLVAPIGFLCDHVETLYDIDIELTQ
                     LARTKGIQLERIPMLNASAPLIDILTSVVEAHESSRVH"
     gene            complement(17140..18165)
                     /gene="hemE"
                     /locus_tag="PK589_13750"
     CDS             complement(17140..18165)
                     /gene="hemE"
                     /locus_tag="PK589_13750"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNJ20719.1"
                     /translation="MNDRFLKACRREPVDCTPVWFMRQAGRYMIEYRRLREKHSILDL
                     CKTPELAAQVTLQPIDRFALDAAIIFADILLPLEPMGLSLEFAEGEGPIIHNPLRDRA
                     AVERLKMLDDSDLQYVMDAISLTRKMLAGRVPLIGFAGAPFTLASYAIEGGSSRNYIH
                     TKQMMYGEPEVWHRLMDKFARAITGYLRRQIKAGAQAVQLFDSWVGCLSAGDYAEYVM
                     PHVQLIFDGLKHEGVPLIHFGTGTTAILKSMRQAGGDVIGVDWRIPIDEAWAMVGYDR
                     AVQGNLDPVTLFGPMHEIERRVTDILRRAGGRPGHIFNLGHGILPNTPVEHVAATIDL
                     VHKLSAR"
     gene            18540..19556
                     /locus_tag="PK589_13755"
     CDS             18540..19556
                     /locus_tag="PK589_13755"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNJ20720.1"
                     /translation="MSSDLHPLTLPDNGELSKTAEQPAEQATSPTPWLTVLHWFDSWA
                     GIENMKTDAAPATVDWLRAIPLITVHLICLGVFIVGWSWVALGVCAAFYFIRMFAITG
                     WYHRYFSHRTFKTSRACQFAFALLGSSCAQRGPLWWAGHHRHHHIYSDKPEDTHSVRQ
                     GGFLWAHIGWISSKKFFPPRLKSIGDFAKFPELRFLDRFDTLVPTICGFGMFGLGKLL
                     ESYAPGLGTNGPQMLIWGFFVSTVALLHGTCTINSLSHVYGSQRYKTGDDSKNNFILA
                     MITLGEGWHNNHHYYAASTRQGFYWWEIDITYYMLRAMQAVGLVWDIREVPAHVREGK
                     NKLA"
     gene            complement(19604..20074)
                     /locus_tag="PK589_13760"
     CDS             complement(19604..20074)
                     /locus_tag="PK589_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20721.1"
                     /translation="MAQKFGNSRWVQEGFLDNRQDGTVVGRITFAVLGAIDFYLTGNC
                     RGEISGKVIRFKNSRFADEDLAGQVLGDVESPQIGDASLISFDPHPHLVPHPYIEWFS
                     MRKNHYRIELVPEDAWIATEAEIAEIDAASRDIRDRLAPQYGSKSRSADESEWV"
     gene            complement(20076..20954)
                     /locus_tag="PK589_13765"
     CDS             complement(20076..20954)
                     /locus_tag="PK589_13765"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNJ20722.1"
                     /translation="MPQLALLLTTFVWGATFPATKAALEQITPLAFLFLRFLLGMLLV
                     FAVLLLLRRKLTRDAAMLRMSLIASLWLFLGYVLQTVGLHLTTASNSAFITVLYVVFV
                     PLYLRRLSAHTWISNGIALIGLWFLVKPTASANLGDLLTLGSAAAFAAHMVCLERYTR
                     TTDAVSLFAWQLVMMTVAMLGAMWWEQPTAAMFEPSRVLLLGLGVTGILATGAFAVQM
                     WAQQLLPAQQVALLFAVEPAIAAWLAWYFLGENLDAQGWFGSAMILGGVLLGSRVPGE
                     MPQVSPTPVVVRSEGR"
     gene            complement(21049..21252)
                     /locus_tag="PK589_13770"
     CDS             complement(21049..21252)
                     /locus_tag="PK589_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20723.1"
                     /translation="MIYWIHIARAIDRVTLHRDRCSEVPSSISSSDFWEGGWFDYPDK
                     ERALAAMEQAGTTDERKCALCKP"
     gene            complement(21402..21578)
                     /locus_tag="PK589_13775"
     CDS             complement(21402..21578)
                     /locus_tag="PK589_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20724.1"
                     /translation="MPLLIRKYKGNGGVMQEERIDDPDRIERYMRLFEEKDVKKLETG
                     AKTVIEKDEWQLLP"
     gene            21748..22059
                     /locus_tag="PK589_13780"
     CDS             21748..22059
                     /locus_tag="PK589_13780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNJ20725.1"
                     /translation="MPVYEYRCDQCTHRFEATQSIHARPDDTVCPQCTHMGATRILSA
                     FASKVKGSHKPGFEEIKAYDMLHERMDRFSKLPPAMGKRVDVTPDMMSGAVTPTDSPE
                     S"
     gene            22071..22982
                     /locus_tag="PK589_13785"
     CDS             22071..22982
                     /locus_tag="PK589_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNJ20726.1"
                     /translation="MSGRDCRPGLPRHDAHRKAAQGREGESFSMYSRISALLCAGLFS
                     LSTLPASAEVAGSMVIAGHGPEQHVMESLAHAFEKANPRAYIDVVWDDNSKPLDMVKA
                     KQAHIAVTGASANGLHSVQIAWDGIAIMVHRSNFTKEVTKQEVADLFSGKYKVWADLG
                     GPDTKVLLIDRPRNENNRDAFEQLLGITGKIPDSTKVIAKDEKVIKTIAGTLPPNSAV
                     TYLSLGQALEAVASGVPVRLLPVDKVEPETPTVKDGRYSLRRPILLISNNDADPLVAA
                     FEQFALSDEGQKIISDSYTPLPKTSSP"
     gene            22995..23450
                     /locus_tag="PK589_13790"
     CDS             22995..23450
                     /locus_tag="PK589_13790"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ20727.1"
                     /translation="MRTFMTVALGSVVALGVLCMAVPSGQAQEGAMVEGAGYTMFNTE
                     SIKGSDTNKLEKDPVCDRSKRPSIGKVEPDEAKPGDKIVIKGENFGSKECFHGVTFSA
                     APKDKIEYQFVNESTIEAVVPDAKAGMSFIIVVAGGGSAQSKPLLIQAK"
     gene            23623..25041
                     /gene="accC"
                     /locus_tag="PK589_13795"
     CDS             23623..25041
                     /gene="accC"
                     /locus_tag="PK589_13795"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNJ20728.1"
                     /translation="MFRKILIANRGEIAMRIIRGCRELNIATAAIYSEADSSGIYVKK
                     ADESYLVGPGPVKGFLDGKQIVEIAKRIGADAIHPGYGFLSENAKFARLCHASGITFI
                     GPSPETIDLMGSKVKARQIAQQAGLPIVPGTEGGVTSIEEALEFVHRINYPVMIKASA
                     GGGGRGLRVVRSDQELRENMEVAAREALAAFGDGSIFIEKYIERPHHIEFQILGDKHG
                     NIIHLGERDCSIQRRHQKLIEIAPSLVLTAKLRAQMGEAAIAIAKAVKYDNAGTVEFL
                     LDHEGHFYFMEMNPRLQVEHTVTEQITAIDIVRNQISIAAGKPLEIRQKDVTLQGHAI
                     QCRINAEDPRNNFMPCTGTVTAYLSPGGIGVRIDGAVYRDYTIPPYYDALLAKLTVRG
                     RTWEEAVSRMRRSLEEYVLRGVKTTIPFMKNVMMEQDFQAGRFDTSYLETHPDLYQYE
                     ESEEPEDLVLAISAAIAAYEGL"
BASE COUNT         5546 a         7532 c         6811 g         5074 t
ORIGIN      
        1 ggactttgtc cgccacggcc acggccgcga cggtgcccgc cgagattttg agaaattgtc
       61 gccgtgaaaa taacatagat gactccttat gtaaggggtg gaggattggg ggataggacg
      121 cacggtgcag gggcctcctt tctcttgaag caaaaaaaag gcaaagggat aatagcgggc
      181 ctctgcgaga aacaacgtcc ctattcaggg aaaatcctgt cattttttaa tgacagcgcg
      241 tctacatgaa cgggcgtgtc actaagggat atccctagtt gattccaaag aaggacagtg
      301 gtgaagtagc aggcggcgaa cttctagaga aaggagttca agacacgtgc aacgggccgt
      361 cattgcagac gatcatcatt tgtaccgtcg tggcacccgg gacgtgatcc aggaggtgtt
      421 tgcgccagaa gtcatcgctg aggcgaatac aggaacacaa ttactcaatc tcgtgaaagc
      481 ctgtaattgg gatctgtgca tcatggacgt gtcgatgccg gtaaagtccg gccccgattt
      541 agtagaagaa gtgttacaaa tccgtccgtc catgccgatt ctggtcttgt cgatgcaccc
      601 cgaagagcat tatgcagtgc ggatgatccg atccggcgcg cgggggtatc taaacaaatc
      661 caccacggat tccgccttac tgaccgaagc cattcgctca ttgctcgccg gacacaaatt
      721 tatcacgtcc accgtggcag aatgtttagc cgacaccgtc tgtgccggat cacacccatc
      781 ttcagccctt catcaggttc tctcgaacag agagctccaa atcttccaaa tgctggccgc
      841 gggaacaccg atcaaggaaa ttgccagcac cctgtttatt agcgctgcca cggtcagcac
      901 gtatagaacc cgcgtattga ccaagttggc tctcgacagc aatgcggatc tcgtgcgtta
      961 tgcgctcgag cattcgttga ttgcctgacc tgtcatgcag ggaagtcacg ggcagctcct
     1021 gacagtgaat ccgtcaagat gcctgaagaa gtgattgcct tccgttccct cttgccccta
     1081 ttgattccca actcccacgg tctcgggttg gagccagccc cgtacgagct ccggttcatc
     1141 tgccgcagtg agtcggatac gcttttgacc gcaccaggca caggtccagc gctcgaactc
     1201 gcattggcct tggctgttat cggaaaccca atggtgtgta tgaggagttc tcttcgaggg
     1261 cttggtcccg acaggaaacg agtaggtggc aacaaatttt tcgttcagca tgaggccgag
     1321 gataccgaag ttctgcggca aggtgggtta ggaagacccc tggttgtaaa gattgtaacc
     1381 cctcattgca ctaccggagg ctcaaggtcg aggggctccc acctcgcttg agatcatttg
     1441 ataccccagt ctcatcctga agcgttgctc tggtcttctt tcatgggtcg tggcactccc
     1501 cactcaaaac gcacgatctt ccattgatgg gcctccttga cgaacaggta tcggacatca
     1561 ctcacatact cactgtaccc ttccacaaag ccattgatgg aggcggtgtt accgttcact
     1621 tttctggaga ggaaggtgct gccgcgcggt tccatcacaa cactgttctc ctggatcaat
     1681 gtcacaaatt catcgtatga cacctgttct tgcgcccgta gtgatagata gctatacgcc
     1741 tttccatact caccatcttc gatcgcctgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnc ccgtagtgat agatagctat acgcctttcc atactcacca tcttcgatcg
     1921 cctgaaggtg ggctcgaatg atgccaaccg gtttcacggt ttgtccccac caatacccca
     1981 ggaccagcat ccccaccacg aatatgaaaa ccaagattcc cggcaggtga atgcgtgtgg
     2041 cttgggcggt ttgcacccgg ctaggggcat cgaggatttc aaccggtggt ggaacaatat
     2101 ggttgggtcc ctgagggacg gcgccctggt aatgatagag aagagtcgcc cgtatcgggg
     2161 cttcacattt aggcgcgtgg ccgaaaaact tgagcttctc aagcatagcg tctccataga
     2221 gtcggaagag ctcaggcatg gtggtcagtc cgggtaatct gttcaacagc ctcgaacact
     2281 cttgaacccg gtgagggcga cagtcagacg aacaacgcga gcgtagaaag acgactcgta
     2341 tcgatcgccg acgcaattcc gaatcacctg gccaatcacc atgcgcacct atccacgcaa
     2401 gtcagcggca caaagccgct acgcgcggga tcacgagact aggatggcaa ggagatcaaa
     2461 tgcggtagac ggagacacgt tggtgaggac gaagcgtcag aagctgcgat ggaatcgaga
     2521 gggacggtga actatcggcg agatggatga ctgaggggat cagcacgatg gggaccacgg
     2581 aactcgtcac ctgctttggc agcggaggaa tttccggctt gagggtcagg ctcagctggg
     2641 aaagcgcctg ctcatcctga tcgctcgtat ccaacaggct gttcacttgt tcagtgaatg
     2701 agaccccacc gaaaaacagc catattgcga gcaggctcca gatgacgcgc cggagggtga
     2761 tggttcgacc actcatcgtt gaacagtaat acgaaccaca caaggagtca atgatgccac
     2821 tgtcgcgaca ccttgctccc ttctcatgga ttcatcgggc tctggggaaa aaccaacggc
     2881 ttcggtgccc cgcctgctgt ctcacgcgag cgctgccgaa atctgagaag cttgcggtag
     2941 ggatgccccg tagaatgtgg ccgcatgcct cgcctttggc tggcgaccga accaattgag
     3001 atcgtagcga tcattctgat cgaacaggcg acgaagattc agtgttgaca gaagagcggt
     3061 aaatccttcg acccgaagac ttctgttgcg aaagactgtc gtctcgacct gcaatgtttt
     3121 ttctggtgac ccatcgaatg gcacgataat aaacacgtga taccggtctc cgacttgccg
     3181 agacaaaata ctggccaggg ggaaactgtg aaccagatac cggatcaaca ccgtgagttt
     3241 ctcttcggcg attcgttcgt ggtaccaagc catggtgatc acctccagtt cctgcttctc
     3301 ggggcgccgc actgccgatg gatcgccaag cgaaactgcc ctgctcatcg ttcagaccaa
     3361 cgggctggcc tggtcagcaa cccaagcgtg gcgagaccac agatcaatgc taagagcagt
     3421 agactcgcca ccagaaacag ccatgctttt atctcttgcg tcatgcccgt atcctacggc
     3481 ttcccggttc ggccatggac ctagggaaac cgctggaatg tgattaggag tactctcgtt
     3541 tacaggctct cgtagagctg ccgatgcgcc ttacgacgtg aagttgccga ggaatgtgcg
     3601 cccagatagc gagcttatgc agaagtgtag aaagagaggc ggttaacaga ttattgggga
     3661 tggacgaacc ggacggaatg gcttgcgtgg atgagaagga aagtggtgga gggcagggga
     3721 gttgaacccc cgacccctac gttgcgaacg tagtgctctc ccaactgagc tagcccccca
     3781 caaccaggcc gcattataca caaccggcgc cgcagtctct agcgggctac ggaaaaagtc
     3841 tcgacgaggt cttctatcag ggatagtgga aaccggtcac cggagattcg ggctccgcca
     3901 caaagacccg ccgtccctcc actcgcaacc gcccttcggc atagagctgg atggctcgcg
     3961 gataaatgcg gtgttcatgc tcgagaatgc gagccgctaa cgtctcgggc gtgtctccct
     4021 ctgcgatcgg tactgccgct tgaatgatga tcggtccttc gtccacccct tcggtgacga
     4081 agtgcaccgt acagccggca attttgcagc cgtgttcaat ggcttttttc tgcacatcta
     4141 gccctggaaa cgacgggagg agtgacggat ggatattcat catgcgattt tgatatgccg
     4201 tgaccagcac cgctgtgaca atcttcatgt atcccgccag cagcaccaga tccacttcat
     4261 gcttctgcaa gacctcttgg acagcccgat cataggcctc acgactatcc ggtctgccgg
     4321 cgaagggttt gggatcgagc cagaccgccg gcgcgccatg cttgcgcgct ctttccagag
     4381 cgacggcctc ctgcttgttg ctcagcacca ccgcaatctc ggccgacagc gcatgctgtt
     4441 cgatggcgtc gataatcgcc tgcaggttcg acccgcgtcc cgaggccaat acaccgagcc
     4501 gcaccagccg aggggcttta gccgacatac tcgaccaccc cgtcttcggt ctgctgcgcg
     4561 accatctcgc cgatgtgata ggcctgctcc cctaactcgc gagccttcgc catgacccgg
     4621 tcggcatggt cgggagcgac aaccaggatc aagccgatcc ccatattgaa gacgcgatac
     4681 atttcgtcta cggccactga tccgcgttcc cggatcgcct cgaaaatcgg cagcaccggc
     4741 caggagccac ggcgaatccg tgcgccgcaa cgcgccggaa aaacccgtgg caggttgtcg
     4801 gtgatgcccc cgccggtgat atgggcaatc cctttgatcg gacaagattc catcagcgac
     4861 agcacctgct tggcgtagat gcgcgtgggc gtcagcaagg tttccccaag tgtcccgccc
     4921 aattccggca tggccgtggt gacggtcagc ccgctccgct cgagcaacac cttcctgacg
     4981 agggaaaagc cgttgctgtg cacaccgctc gaggccaacc cgatgaccac atcacccgga
     5041 acgatctgct ttccatcgat gatcttcggc cgatccacca cacccacggc aaacccggcc
     5101 aggtcgtact caccttcgcc gtaaaacgag ggcatctcgg ccgtttctcc cccgatcaag
     5161 gcacatccgg cctgtcggca gccgtcggaa atgcctttca ccaccgccgc cgccgtcgcg
     5221 atcgacagtt taccggtggc aaaatagtcg aggaagaaca gcggctccgc gccactcacg
     5281 gcaatatcgt tcacacacat ggccaccagg tcgatgccga ccgtgtcatg tttatccatc
     5341 agaaaggcga tcttgagttt cgtcccgacg ccgtcggtcc ccgacacgag caccgggtcc
     5401 tggtaccgat tggcctggaa ccggaagagg ccgccgaagc cgcccagatc cgtcatcact
     5461 tcagggcgaa acgtggcccg cacatgcggc ttgatccgct cgacgaattc atcgccggca
     5521 tcgatatcga ctccggcatc acgataagta gtcattgggg tcgcatctta acgagccaac
     5581 tgatagcggg tcaacaatag tctcacacac tggggcgcta cgtgagtccg tcagacatca
     5641 acacgagaag ggctgctcaa acagttcatc caacgaggcc gcaggggaga aagaaccgga
     5701 ggcgtaccct cggggtacgt cgcagcgagc gtcgcgactg agaacgcagc tggcggtcgt
     5761 tttcagcaac ttgcctacat ctcagggcgc atctcgacat caagtaactt aatcttccga
     5821 tgtaaatggc tgcgttcgat cttgaggtct tccgccgtgc gggaaatatt ccaatgatgc
     5881 tcgcgcagtt tgcgggcaat gaattccttt tcaaaggcat tccgcgcatc gcgaagcgaa
     5941 tcgtacggcc tggtcagcaa ggtattgacc ggttgagcgc cggcggccga ttgggcggct
     6001 gacatttgag ggcgtacttg aagtgcgacg gaagcatgcg ccgcctcgat gaccggcccg
     6061 ggcaccatga tcatcagccg ctcgatcaga ttccgcaatt cacggatatt ccccggccat
     6121 tcatactgca tgaacacgtc catggcctcc ggtgacacct gcttgatctt caacccctgc
     6181 tcttccgcat gcacgcgcag aaaatgtttc accagcaagg gaatgtcctc gcgacgatca
     6241 cgcaaagtgg gcactacgat cggcaccacg ttcaggcggt agaacaagtc ttcacggaac
     6301 gttcccttct cgatttcctg cagcaaatcc ttattcgatg cagccagcac ccgcacgtcg
     6361 accttgatca gcttggtccc gccaacccgc gtaaactgtt gctcctggag agcccgcagt
     6421 actttggctt gcgtgctcaa gctcatgtct gcgatttcat ccaaaaataa cgtcccgccg
     6481 tcggcctgtt cgaactggcc gcgcttcatg gtggtggctc cgctgaaggc acctcgctcg
     6541 tggccgaaca actcgctttc aatcagggtc tcgggaatgg ctgcacagtt gaccgccaca
     6601 aacgggcgag tcgccctgtg gctgtgttga tggatggctc tcgccaccaa ttccttaccg
     6661 gtgccgtttt cgccgccgat cagcactcga ctattggtcg ggccggccgt ttcgatcagt
     6721 tgcttcagtt gctgcatgac cggcgcctgg ccgatgagct cgaacttccg ctcgactttg
     6781 gtgcggaggt tgcgattctc ctgctcgagc cggtgttgat ccaaggcatg tttcaccctg
     6841 agggtcacat tttcgaggga cagcggcttt tcgatatagt cgtacgcacc cagtttgatc
     6901 gccttgaccg ccgtctcgat cgaaccgtgc ccggacatca tcatgacttg agtgctgggc
     6961 accagttcgc gcagtcgctt gagggtctcc agcccgtcca tctcgggcat ccagatatcc
     7021 agcatcatca gttcaggcgg actgatcgcg caatgtttca gggcctccat cccgcttttg
     7081 gccacactca cgtcatatcc ttcgtcctcg agaatactgc tcaaggaatt caggatggag
     7141 acttcgtcat caacaatgag aatcgaagca gacatgcatc ccccacatac gatccgccaa
     7201 cacccactcg accgtccgtt cccttacacc ggcaattcga aggtaaataa ggcccccttc
     7261 ggctgatgat tccccgcatg aatctgacca tcgtggtcgg tcacgatccg cctgacgatg
     7321 gccaacccca agcccgtccc ggtcttcttt ctggaaaaat acggcacaaa gagtttgtcc
     7381 tgatcctccg gcgcgatacc ggtgccttca tccgccaccg tgaccaccgc gcggcgttgc
     7441 ttcgtatcgt accgggtcgt gatccacaag cggcccttgt ggttcatggc atggatgccg
     7501 ttatcgcaca gattcaccag gacacgcttg atctgttcgc gatcgaaatt gaaaggcggc
     7561 agatcggcat cgagcgacac cacacagatg atttctcgat gggcactttc gtagagcgcg
     7621 acgacttcct tcacgacatc gtcgagcgaa ttcatcgtca tatgcggcgc cggcatgcgc
     7681 gcgaatttcg aaaactcatc cagcatctgc ttgaggctac cgacctcgtt gacgatcacc
     7741 tgcgtggatt cgtcgcagat acggtcgaaa tccggtgcct tgtcctgaaa tttcttccgc
     7801 aaccgctggg ccgagagttg aatcggcgtg aggggattct tgatctcgtg cgcgatccgt
     7861 tgcgccactt cccgccaggc cgccgccttc tgcgccttga tcaattccga caggtcttca
     7921 aaaatcagca cgaagccgag atctttattc gactcgtcct tcatgcgtga gcaatgcaca
     7981 ccgatggtga gaaagcgccc ctgcaaatcc agctgccctt ccagcgcgat gttatcccgc
     8041 tggtcggcca gcatgcgatc gtagacggat tgaaacaggt cgagttgata ctctttgaac
     8101 acctcgttgg cggagcgccc gcgaaaccgg tcggcccaca accccagcat gcgttcggcc
     8161 gatggattaa aggtcgtgac aatcccctgc cgatcgatcg acaacacccc cgcggcaatc
     8221 gtatcgacca ccgtttcgat ataggcgcgc cgccggtcca gttcgaggtt ggattggcgc
     8281 aaggagacgt tcgcctcttc aaccttgctt ttgctgccct gcagatcggc ggtcatccgg
     8341 ttgaaggatt ccaccaacgt cccgatttca tccgtcgcct tcgcttcgat ccggaccgat
     8401 aaatcccctt gggcaatggc ctcggtcgcc tcggccaacc gctgaatcgg cacggtgata
     8461 ccgcgcgcga cataaaaccc gaaccaggtg gcgctgaaca aaatcatcac cgtgatcacc
     8521 gccactaaca gataggcgcc cgccttgata ggattcttca tcgccttcat ctgtttgtac
     8581 tcggcatatt gccggccgat gctctccatc ttgccgagca acgattccgg cacataggcg
     8641 tccacgacga ccaccccgtc gatttccccg cgccgcacgc tggaagcgat cggcacgccg
     8701 gcacgcacga gccggccggt ctgcgcttcc tgcacagagg tcaactcctg tttgccattg
     8761 atcacctgca acaccagctg gccgatcggc agatcgagta cggtcaccgg cacctcagga
     8821 tccagggcct tcgtgagcgt ttccattttc gcggaaaaca cctcaatgcc ggccgtggcg
     8881 tattcatcgc gtttccgggc tatcgctgcg actaacagat cccgctgctc cggcaacagc
     8941 aattcctcgc gaaagatctc atggctgatg gcgcgagcgc tgttgatcgc caacgacaca
     9001 tgtccggcat ggtgcatgcg cgccacctcg taggagtctt tcatcacatg ctcgatctga
     9061 tcgttaaacc agacatcgac ggctttgttg accaacccgc tggcgaccaa ggccaacaac
     9121 acggtcggaa tgagggagaa tccgatgaag gccgcaacca acttcgcccg aaacccggag
     9181 cccaccagac gatgccggcg ttcaaaatag gctttgatga ggttgcgcga cagaagcaac
     9241 gtgaggacga cgaacccgat caggtcgaga ttgaccagca gcaggacaaa ggcatagccg
     9301 gtgctgggaa gaaaggaatc ggattcctct ccaaccggaa cggccatttg tgcatagtag
     9361 aaggtcagcg ccaggcaggg cagcagcagg atcaggacga tccagacggg ccggacatgg
     9421 cgcttgcgcc gctcgctctc gatcgaggcg ggcgacgacg gctctttttc acgcagcaca
     9481 cccgggggga tgagcttgct catgtccgtc gattcaggca tcggcatgac tccgaatcca
     9541 tctcggaccc tttgggttct gccacactat aacgaattcc tgaggaaaga ctcaaaagct
     9601 gtgccgaagc catcgcgctg gtcggaaaca tgaacaagac actgagagag gttgagaagc
     9661 cggtcgcaac caggaggaag tcaaacccgg tggaatagcg gaacgcgaag agggcgatca
     9721 caggttacgg ggtatgaatc gatgatttcc cggaagtcag gctgacgaac ttcatcctca
     9781 aggcatacag catgtccttc atcacgacct gctcgtccgg cgtgagattg ccccgtgttt
     9841 tctcttccag catggagagg agatcgatga tttccttcgc ttgcggaaga ttcagcggca
     9901 tagccggttg ctgaggatcc aattgctccc ccatgagcat caaagccgag ctgcccatgg
     9961 aaatcacgaa ggaggagaaa ttgaccggca gatgcccggc atggccatgc gcgtcagcat
    10021 gatgggactc ggctgctgcc ggttcgggag caggaggcgt gggagccgag gctgcagcag
    10081 gcgcctcttc accgcggcct cgacgatcgc ggatgacgaa tccctgatcc ttctcatcac
    10141 ccatgtgcgc aacctcccgt cagtccatca taaaatgcag gacggtaccc taccacaggc
    10201 cttgagggtg cgcaaggcgg cgggccccgt tgaaggtgaa gataaactca gttagagtgg
    10261 cgccacgagg ggaaaccgca tgtccgaacg tttcgataaa gtggtggcga tcatggcccg
    10321 gctccgggca cccggcggct gcccatggga tcgcaagcaa acgcatgaat ccctcaagcc
    10381 ctacctgatt gaagaaacgt acgaagcgct tgataccatc gaccggcggg atttccctaa
    10441 actcaaggaa gaactcggcg atgtgttgct ccaggtgctc ttccacagcc aaatcggaac
    10501 ggaagccggc acgttcacca tggatgacgt gctccaggag ttgggcgaca aactcgtgcg
    10561 ccgccatccg catgtcttcg gggagaacgc gtccggcacg ccgggtctga attccgacca
    10621 ggtcgtgcat cgatgggaag acatcaagcg agccgagcgt aagaatgccg gaaaacccga
    10681 ttcggtcttg caagatatcc ctcaagccct cccggcgtta cttcgcgcct atcaaaccca
    10741 ggttcgcgcc gcacgggtcg gtttcgactg gccggacagc ccgcaagggc tcgctgatgt
    10801 cctcggcaag gtcgaggagg aaatagggga acttcgcaaa gccgtgagtg acgcgggggc
    10861 tatgtcccag cctccatcgg aacctaccgc ggacatggcg gcggaattgg gcgacctgct
    10921 cttctccttg gtcaacctgg cgcgtcacat caaggtgaat ccggaagaat cgctgcgtca
    10981 ggccaccaac cggttcacag ctcgcttcca gcacatcgaa catgaagcgg cgcgcagcgg
    11041 gcgcagcgtg cacgatctca cgctggctga aatggatcac tactggaacg ccgcaaaaca
    11101 attggaatcg gccgatcgtc ctgcggcagc ctccccgctc gcttctaaag gagaggcgtc
    11161 gcagacttcc ttatgagcag tgaacaggat ccatacgaag agggcaagcg agcgggactg
    11221 catccgctgc tcgtgctctt cggagccatt ctggtcatgt ggctgctgat caatcttgcc
    11281 gttcccagcg gaaaacaaaa gcaggcggcc ggaccggaaa tctctcaagt cggcacggaa
    11341 gatccggatg ccgcgccggt catgtacaaa gtgcagacaa cgctgacgga atttaatgcc
    11401 attagcatcc tcgtcccacc ccagtcgacc atgagccagg tcgtcggtct gttgaaacaa
    11461 ttcaaacagg ccagactcaa tcagacactg gccggtatgc tcccggcaac aagccccggt
    11521 cacaaactgg gtgatcaggc agtcgccgac ctgtacatct tttcggaccc taaatatgcc
    11581 tcgccggaag tcgtcggcac gctggcgcgc ggggcccatg ctccgggcaa cctctatccg
    11641 caggctatcc cgtttgaaga agccatggaa caggtacggg ggcattaccg catcgacctg
    11701 aacgacacga gtaatccaga caccgggtcc atcggatttg ccgatgattc cggggtgcac
    11761 agcaagcaat atcgccggat tttttgagca gatctctcga aatcccacgc ctgacgcgcc
    11821 ttcgctatcc ccaagctaaa atcggtcagc aaatatcccc ctaggggttt tcctcttcta
    11881 cagcatctag tggcctgcgc caggacgacc cccaatgcct aaaacttgtg caggccgctg
    11941 aaggaccagc ccacgaaggg cctcgagccg ccataggccc cctatacaca tccttatcca
    12001 cagattctgt gtgtgagata accgtgatta tatacttgac acacccacga gtcaaggcgt
    12061 acacttcgac cataacgagg tgatgccatg gcgaagaatc ttctcactca gccctactta
    12121 accgatgaac tgaaggccca ggacttctta attaagatgc gctggcctga aggcatcacc
    12181 tgcgttcatt gccaaggcga gaaggtctat acgctgaacg tgcgcggctc aaagcgcaga
    12241 gtgttgaagt gtggcaagtg ccgcaagcag ttctctccga ccgttggaac gatctttgag
    12301 gattcgcata ttcccctcac caaatggttt atgaccattc acctgatgtg ctcatcaaag
    12361 aagggcatca gcgcccatca attgcatcgc atgcttggcg tcacctacaa aacggcatgg
    12421 ttcatggcgc atcgcatccg acatgcgatg acgcaattga tgggcgcaac tcccctctca
    12481 gggattgttg aagcggatga gacgtatgtg ggcgggaagc gcatcggtgc gggtatgggc
    12541 tatcgggatc ataaagtacc tgtattcgct ctcgtgcaac gtgggggcga agtccgatct
    12601 atggtgatgc ctcgcgtcac ggcgaacacg ctcagggctg cgattagaac caatgtgaag
    12661 cccactacgc atgtcatgac cgatggggcc accgtctata ccaacgtgtc taacgatgtc
    12721 gcctcgcatg agacggtcaa tcattacgag aaagaatatg tgcgcggcaa cgtccacacg
    12781 aacacgattg aaggctattt cagcctgctc aagcgtggga tcaccggcac gtatcaccac
    12841 gttgcggaga aacacttgca ccgttatctg gcggaatttg atttccgtta caacgcacgc
    12901 aaggttgaag attcggtgcg cacgttctcg gcgatccagg gcattgaagg gaagcggttg
    12961 cagtaccgat gacttaccga gaagagggct tctttgaagc ctttcctttt acggtggagg
    13021 cctttttcga ggaaggcttg gcggtaagta gcagcttgag agcttcctca aattggaggg
    13081 gactcagaga aacggggttt tccttcggca ttccacgatt gtacatggat cactccagcg
    13141 cttgcaaaag agtattagag cgttatagta tttgtgggaa gtgactagcc ggtacttcgc
    13201 gccatcacac tagcaactac cgccgcaacg cgtaatactt gctagggatg ggtcgtagag
    13261 agcccttgag cagccattgc actggccgtt tgggggcttt tcttttagcc atacgccttc
    13321 ctcctttctc caaccagcgg gggattccgc tgtgccaggc agtaatcccc gtcactgcct
    13381 tggagtcatt cagaaggctt acgcgaagcc cgtactagtc actccccggt ttatttgagc
    13441 atctctactg tatcgccctc ccttgaaagc ctaccggcct tcactagccg agtaattgca
    13501 gtcctcatat tgttcccgta acggcccatc ttttctttca tgcccttctc ttctagaatg
    13561 gctcgcagct tgcgaatacc tattggtgca tccttttcgc tcaccaccat catcaccgca
    13621 tcagtaagcg gcaacgatga aaacggaaga ttcatcggct tcgatggaac gctaggcccc
    13681 ccacatcgcg gacagtattt atgcgccttc atctgccagg accgcagatc gtagtccagg
    13741 gcggtaacgc gctcttgaag ggccttgacg gttgcgctta attcctcaat gctctgcgtc
    13801 tgctccatgt tcattccttc tcgcccgaag gcgacggttc ggactatacc atgagccgat
    13861 agacccatgc aactcagtct gttggacaaa ttcaatgtcc gacaggctaa agttatctgt
    13921 ggataacgtt cattgacaga atgtaacctg cgcggcctac aggagaaatc ctatcccgaa
    13981 tcatttgggt gcgtgaagta tataatcacg gagataacga gagaccgccc cctccgaacg
    14041 actatggcga atagatcttg gaatggccgt cagggagagt ggacagagca ctgattgatg
    14101 cgggatgcgt ggaaggaaaa cgcagggatg gttcaaaaac aggtacgact gaggcgcctt
    14161 gcctcccctc acgcgtcgat gaatcagacc gccaaaccga tcgtctgttt gatttcgctt
    14221 cgcaaggtct cctgctgatt ctggacccaa gaatcgagct gcgcaaaatg atgcgccagc
    14281 ggtccggtga agggaggcac caggacatcg gcgcgctcct tcaaccaccc ttcctcctcg
    14341 gcgatgcgga gcttttggtc gccgatcttg cgcgacagca gcttattcag catcaactgc
    14401 tggcccgggc tgccggtcac ggttcccagg agcttcgttt tcacatagtc cagctcttct
    14461 gccagcgcga tcttcacgcg aaccgcatga gtggccgccc aggtcgcccg ttggatggaa
    14521 tagtcatacg aaaacaccgg ctcccggggt tcgcgaaacg gcccagctac actggagatc
    14581 actaactggt catttaacat cgtccatgtc cttctgttgc ttcgtctctc acgattccgc
    14641 ctctcgtttg gaggctgttt ggaggtagcg taacgtctgt gccgccgctt ctgcgccgtc
    14701 tctgatgcaa tcgggcaaac cgacgccgcg atatcccgcg ccggtgacgg ccaatccgcc
    14761 gaaacgtgaa agcgcagcat cgagctgtgt cagtcgatcg agatgcccga gcgtatattg
    14821 cggcatcgcc cgatgccacc ggttgacctc gacataatgc ggtgtcgcct gcagaccgac
    14881 gatagacgcc aattcttcgc gcacgcaacg gaccaatgcc tcatcgtcct gctgcaggat
    14941 ggcctcgcgt cccaccccac cgagatagca gcgcacggac acctcttcgg gaggcgctcg
    15001 gtgcggccac ttgatggacg tccaggtcgc ggcaatcagg tcacggccct caacccgagg
    15061 aaccacgaaa ccaaagcctt gcaagcgggt tcccaccgca gcggcggggt agatcaagga
    15121 aatggtggcc gtcgaagcgt agggaatcgt ctccatcaat ccagccgcca gcgggctcag
    15181 cggccgaacc aggtcggcgc tcacataggc cggtgtcgcg agcactaacg cttccgcgga
    15241 cagcgccgtc ccatcggtgc agatcacatc gtacatccag cgtcccgcct ggtgcgagcg
    15301 tacccgaagc gcttccgctt gcacacccga cttcaagacg ccgccagtct cccggatacg
    15361 cgtggtcagt ccggcgacga gatccgcgag gccgtttttc agcgtgacaa acatggtatg
    15421 ccgaggacgc ccatcggagg ccttttgcgc cctggctcga cgagccgcca tcatgccccg
    15481 aatcacgctg ccatgtgtct gctcgagctc ataaaaccgg ggaaacgtcg cgcgcaggct
    15541 catctgctcg gcatcacccg cgtagattcc cgccatcagc ggttccatga cccgctcgca
    15601 ggcatgctgt ccgaatcggc gacggaaaaa ggaggcgagc gattcatcct ccgtggaccg
    15661 acgcggggga atcagcacat cacaacccat ccgggccagg tcgacccacg acagcaatcc
    15721 gctgcgaaaa aacggaccta attgagtcgg cgtaaaggtc acgagccctt cgggcaactc
    15781 gtgcaactgc ccgcctctca atacgctggc ctttttttca acagggttcg tattgataag
    15841 ctcatcggcg atttcgagcc gccgacagag gtccattgcc cagggcttct gagacaggaa
    15901 ggaatccgga cctgcctcca taaccaaccg gccgacacga tgggtgacga tctttccgcc
    15961 ccagacaggc gcggcatcga ggatggtaca ggacagcggc acatcggccg ctgcggcttg
    16021 ctccaggaga gcaaaggccg tggagaggcc tgagatgcct ccaccaataa tgaccactga
    16081 ccgtgaagtg cgcgccacag gtggtgccgc taatgaacgc gcgaggactc gtgtgcctcc
    16141 acgaccgacg tcagaatgtc gatcaggggg gccgaggcgt tcaacatggg tattcgttcc
    16201 agttggatcc ccttcgtacg ggccaactgc gtgagctcga tatcgatgtc gtacagcgtt
    16261 tccacatggt cgcaaagaaa accgatgggc gccacaagca catggtgatg accggcttgc
    16321 gccaagtctt ccagcgtctc ctcgacagaa ggcccgagcc acttctcgcc ggagcgcccc
    16381 tgactttgat aggcgaaccg cgtcggttgt ccgccgagtt gcgcacatac ggcttggacg
    16441 gtagcgccga cttcctccgg gtaggaatcc ttcatggcca ccacccgctc cggcagacta
    16501 tgggccgtaa agagcacgga cacttgcgcg cggacctcca cgggaaatcg ctccagcgta
    16561 tggcggatat gcgcgacgat cgcggcgatt aacaacggat gacggtgcca gctcgtcacg
    16621 tagctgatcg gcgtctcatc tgccaggtcc gcacgcgcct cttcgacctt cttcacatag
    16681 gcgccaatgc tgagcgacga gtactgaggc gccatgcaca agcccacgat atgatcagga
    16741 tccgccttga gcagttcagc gtaggtgtcc ctgatgaacg gatgccagtg ccgcaatccc
    16801 acgtagcacc gaaagcgcct ttcgccggat gcgttcagtc gctgttccaa ggcggcggcc
    16861 acctcgcacg ttatgtcgag tacaggagac ttcccacccg tcacacggta ccgcgtccgg
    16921 atttcctcca ccaactccgg aggggtcgga cgcccgccgc ggacatccag aaggtacggc
    16981 tctacattct ccagactatc gggcccgccc atggccatca gcaacagggc aatcgggcgt
    17041 ggtgctgccg acatccgaga cctcgtcagc cgtgaccagg aagacgtctc tcgcgaatca
    17101 agcgcctgcc gggcaatgag cgcctacgtc tcaccagatt cagcgggcgc tcaacttatg
    17161 gaccagatcg atcgtggcgg ccacatgctc cacgggagta ttggggagga tcccatgccc
    17221 gagattgaaa atgtgcccgg gtcgccctcc ggcccgccga agaatatccg tcacgcgacg
    17281 ctcgatttcg tgcatcggcc caaacaaggt gaccggatcg aggtttccct gcacggcgcg
    17341 atcgtatccc accatggccc aggcctcgtc gatcgggata cgccaatcga cgccgatcac
    17401 gtcaccaccg gcctggcgca tcgatttgag aatggccgtc gtacccgtcc cgaaatgaat
    17461 cagcggcaca ccttcgtgct tgaggccgtc aaaaatcaat tgcacatgcg gcatcacata
    17521 ttcggcatag tctcccgctg agaggcagcc gacccaactg tcgaagagct gcacggcctg
    17581 ggcgccggcc ttgatctgcc gacgcaaata cccggtgatc gctcgcgcga acttatccat
    17641 cagacggtgc cagacttccg gctcgccata catcatctgc ttggtgtgga tatagttccg
    17701 tgaactgccg ccttcgatcg cataactggc caaagtaaac ggcgctccgg caaagccgat
    17761 cagcggcact cgaccggcca acatcttgcg cgtcaggctg atggcatcca tgacatattg
    17821 gaggtcgctg tcatcgagca tcttgagccg ctccaccgcc gcccgatccc gaaggggatt
    17881 atggataatc ggcccttccc cctccgcgaa ctccagcgac agtcccatcg gttccagcgg
    17941 cagcagaatg tcggcaaaaa taatggctgc atcgagcgca aaccggtcaa tgggctgcag
    18001 ggtgacctga gctgccaatt ccggcgtttt gcacaggtca agaatcgagt gtttttcccg
    18061 caatcgacgg tattcgatca tatagcggcc cgcctggcgc atgaaccaga ccggcgtaca
    18121 atcaaccggt tcgcgccggc acgctttgag aaatcgatca ttcataatgg ggcgcatttt
    18181 agagggccgg tccgaacaga gtcaaacaat cttgcgcagt gcaccgactt gagccattcg
    18241 tggcacgctc atcccgctcc ccggcctgta cctacggctc gggtcagggc agcagtgtgg
    18301 ggaaaaaggt tcgtcgtgaa aggcgaaaaa actcacgaca ttttcctacg aacctgtgta
    18361 taatggatgc acgtgtcttg agggctgagt ctatacgctc accgatcgac accatccgaa
    18421 acattcagcg gcctgcccca tcgcatcaat cgcaagcgcc gcaacgatta cgtcacgcta
    18481 gtgcaacggc ggcgcgaccc ctcggtaccg catcctattc gcacaactgt ggaggtggca
    18541 tgagtagtga tctgcatccg ctcaccttac ccgacaacgg tgagctgtcc aaaactgccg
    18601 aacagcctgc agaacaggcc acgagcccta cgccctggct cactgtccta cattggtttg
    18661 attcctgggc cggcatcgaa aacatgaaaa ctgatgcggc tcccgccact gtcgactggc
    18721 tgcgggccat ccccctcatc accgttcact tgatctgcct cggggtgttt atcgtcggct
    18781 ggagctgggt tgcgctcggc gtctgcgccg ccttttactt catccgcatg ttcgccatca
    18841 ccgggtggta ccaccggtac ttctcccatc ggacatttaa aacgtctcgc gcgtgccaat
    18901 tcgcctttgc gttgctggga tcgtcatgcg ctcaacgggg tcccctctgg tgggccggcc
    18961 atcatcgcca ccatcacatc tattcggaca aacctgagga tacgcattcg gtgcgccagg
    19021 gaggatttct ctgggcccac atcggttgga tcagctcgaa gaaattcttt ccgccccgac
    19081 tgaagagcat cggcgacttt gccaagtttc ctgaactgcg gttcctggat cgcttcgata
    19141 ccctggtgcc gacgatctgc ggattcggca tgttcggatt ggggaaattg ctggagtcct
    19201 acgcaccggg actcggcacc aatggtcctc aaatgctgat ctgggggttc ttcgtctcca
    19261 ccgtcgcact gctccatggc acctgtacga tcaactccct ttcgcacgtg tatggatcac
    19321 agcgctataa gacgggtgac gatagcaaaa acaatttcat tctcgccatg atcacgctcg
    19381 gcgaagggtg gcacaacaat caccactact atgccgcgtc aacccggcag gggttctact
    19441 ggtgggaaat cgacatcacc tactacatgc tgcgcgccat gcaggctgta ggtctggtat
    19501 gggatattcg cgaggtgccg gcccacgttc gagaaggcaa gaacaaactc gcctgagtca
    19561 cagcgggggc ggccaattca ctgcccgccc ccgacactcc gcgttacacc cactccgatt
    19621 catccgccga ccgtgatttg ctgccatact gcggcgccaa gcgatcacgg atgtcacgac
    19681 tcgcagcatc gatctcagcg atctctgcct ctgtcgcaat ccaggcatcc tccggcacga
    19741 gttcgatgcg atagtgattc tttcgcatcg aaaaccattc aatgtatgga tggggcacca
    19801 gatggggatg aggatcgaac gagatgaggc tggcatctcc gatctgcgga ctttcgacat
    19861 cccctaacac ttgaccggca aggtcctcgt cggcgaaccg gctatttttg aatcggatca
    19921 ccttgccgga aatttctccc cggcaattgc ccgtcagata aaaatcgatc gcgcccaaga
    19981 ccgcaaacgt gatgcgcccg accaccgtcc cgtcctgtcg attatcgaga aatccctctt
    20041 gcacccaacg gctgttgccg aacttttgag ccatatcatc tcccctctga ccgcacaacg
    20101 accggcgtcg gagatacctg aggcatctcc cctggcacgc gagatccgag cagcacaccg
    20161 cccagaatca tcgcgctgcc gaaccatcct tgcgcatcca gattttcgcc caggaaatac
    20221 catgcaagcc aagccgcgat agccggctcc acggcaaaca ataatgcaac ctgttgtgca
    20281 ggcagcagct gctgcgccca catctgcacg gcgaacgcgc cggtcgccag tatcccggta
    20341 actcccaggc ccaacagcaa cacacggctc ggctcgaaca tggccgccgt cggctgttcc
    20401 caccacatcg cccccagcat cgctacggtc atcatcacca gttgccaggc gaacaacgag
    20461 acagcgtcgg tcgttctggt gtagcgctcg agacagacca tgtgtgccgc aaacgccgcg
    20521 gcgctgccga gcgtcagcag atcaccaaga ttggcagagg ccgtgggctt gacgagaaac
    20581 cacagcccaa tcaacgctat gccgttggag atccaggtgt gcgcgctcaa gcggcggaga
    20641 tacagcggaa cgaacaccac gtacaatacg gtaatgaagg ccgagttgga ggccgtcgtc
    20701 aaatgcagtc ccaccgtctg caacacatat ccgaggaaga gccagaggct ggcgatgaga
    20761 ctcatccgca gcatcgcggc atcgcgcgtc agtttccggc gcaataggag cagcaccgcg
    20821 aagaccagca gcatgccgag taaaaatcgg aggaacagaa aagccagcgg cgtgatttgt
    20881 tcgagggcgg ctttggtggc cgggaatgtt gcgccccaca caaacgttgt gaggagaagc
    20941 gcaagttgag gcatggtcag gcgattggac gtactgaatg atgaaccgga tctgccgtga
    21001 acgtgagccg gcgtggatct atcggctcag cactctcgtg tgaatcgatc agggtttgca
    21061 gagcgcgcac ttgcgctcgt cggtagtacc ggcctgctcc atcgctgcga gcgcgcgttc
    21121 cttgtcagga tagtcaaacc agccgccctc ccagaagtca ctactcgaaa tgctggaggg
    21181 cacttccgaa caacggtccc gatgaagtgt gactcgatca atcgcgcgtg caatgtggat
    21241 ccaatagatc atggtacccc gttggactct gtcgtcagga ccgtcgcagc ccaaccctac
    21301 cacgcacttc tcggagagag caatgcgcag gacatatcaa gccgcaacga cccgcgacgg
    21361 aaacacacat gagatgatgg gatgaaccca ggaggtacgt gctagggcag gagctgccac
    21421 tcgtccttct cgatcaccgt cttggctccg gtttcgagct tcttgacgtc cttctcttcg
    21481 aacagacgca tataccgttc gatccggtcc ggatcgtcga tgcgctcttc ctgcataaca
    21541 ccgccattgc ctttgtactt ccggattaac aggggcattt caaccgctcc ttcaattgta
    21601 gctcgcgttt cggcacatag attcggtaca atagtgcaaa gttatctata gggtcaagcg
    21661 tttccaaaca gagaactgct ttatctcggc tagccctcct atgggcagcc atgaagtgca
    21721 ttcttctcca gctgtaagga gtcgacaatg ccggtttacg aatatcgctg tgatcaatgt
    21781 acccatcggt tcgaggccac gcaatccatc catgcgcggc cggacgatac ggtgtgtcca
    21841 cagtgcaccc atatgggggc aacccggatc ttgtccgcct tcgcctcgaa ggtcaaaggg
    21901 agccacaagc ctggatttga ggaaatcaaa gcgtacgaca tgcttcatga acgcatggat
    21961 cgcttctcaa aacttcctcc cgccatgggc aaacgcgtgg atgtaacgcc ggacatgatg
    22021 tccggtgcag taaccccgac cgacagcccg gaatcctaag cggaggtcgc gtgagcggaa
    22081 gggactgccg ccccggcctg ccgcgccacg atgcccaccg gaaggccgct caggggcgag
    22141 agggagaatc gttcagcatg tattcccgta tcagcgccct cctttgtgcc ggacttttct
    22201 ctctgtcaac gttgccggcc tcagccgaag tcgccggctc catggtgatc gccgggcacg
    22261 gacctgagca acacgtgatg gaatccctgg cgcacgcgtt cgaaaaagcc aacccgcgcg
    22321 cctatatcga tgtcgtctgg gacgacaact ccaaaccact cgacatggtc aaagccaagc
    22381 aggcgcacat cgccgtcacc ggcgcctcgg caaacgggct gcactccgtc caaatcgcgt
    22441 gggacggcat tgccatcatg gtccaccgct ccaatttcac gaaagaagtc accaaacagg
    22501 aggtcgccga cctcttttcc ggaaagtata aggtctgggc cgacctgggt ggaccggata
    22561 ccaaggtgct gctcatcgat cggccgcgca acgaaaataa tcgtgacgcc tttgagcaac
    22621 tcctgggcat taccggcaag attcccgaca gcaccaaagt gatcgcgaag gacgagaagg
    22681 tcatcaaaac catcgccggg actttgccgc ccaattctgc cgtcacctat ctgtctctgg
    22741 gccaagcctt ggaagcagtc gcctccggag tgccggtgcg tctgctcccc gtcgataaag
    22801 tggaaccaga aacacccacg gtcaaggacg gacgttattc cttacggcgg ccgattctcc
    22861 ttatctccaa taatgatgcc gaccctctcg tggccgcgtt tgaacaattc gccctgtcgg
    22921 acgagggtca gaaaattatc agcgattctt atacaccgct cccgaagaca tcatctccct
    22981 gataggaggc tttcatgcgc acattcatga ccgtggccct cggttccgtt gttgctctcg
    23041 gcgtcctctg tatggccgtt ccatcaggcc aggcccagga aggggccatg gtggaaggcg
    23101 ccggctacac gatgttcaat accgaatcga tcaaggggtc ggataccaac aagcttgaga
    23161 aggatccggt ctgtgatcgc tcaaagcgcc cctccatcgg caaagtagaa ccagatgaag
    23221 ctaagcccgg cgataaaatc gtgatcaaag gcgaaaactt cggcagcaag gaatgtttcc
    23281 atggagtcac gttcagcgcg gcgcccaaag acaagatcga gtaccagttc gtgaacgaat
    23341 cgacgattga agccgtggtg ccggacgcga aggcgggcat gtcattcatt attgtcgtcg
    23401 ccggcggcgg cagcgcgcag tcgaagccac ttctcattca ggccaagtaa ttcccgccgt
    23461 ggcaggaggc gctgtgcctc ctgcccgatt cgtgcccttc cggattcgcc attttaccgc
    23521 ccttcgtctc gacaactctc ttgaccctat gctaggttac gcgatctttt tgggaggcga
    23581 cccagaggtg agctccggct ccgaacctca cgcaggctca gcatgttccg caagatcctt
    23641 attgccaacc gtggcgaaat cgccatgcgc atcatccgcg gctgccgcga gctcaacatc
    23701 gcgacagcgg cgatctattc cgaggctgat tcctcgggta tctacgtcaa aaaagctgat
    23761 gagtcctatc tcgtaggtcc tggtccggtt aaagggtttc tggacggaaa acagatcgtg
    23821 gaaatcgcga agcgtatcgg cgccgacgcg atccatcccg ggtatgggtt cttgtcggaa
    23881 aacgccaagt tcgcccgcct ctgccatgcc tctggaatca ccttcatcgg gccttctccc
    23941 gaaacgatcg acctcatggg cagcaaggtc aaggcccggc agatcgccca gcaggcaggg
    24001 cttccgattg ttcctggcac cgaaggcggg gtcaccagca tcgaggaggc cttagagttc
    24061 gtccatcgga tcaattaccc cgtcatgatc aaggccagcg ccggcggcgg cggacgaggg
    24121 cttcgcgtag tccgctccga ccaggagctg cgcgaaaata tggaagtcgc ggcacgggaa
    24181 gcgctggcgg ctttcggcga cggcagcatc ttcattgaaa agtacatcga gcggccgcac
    24241 catattgaat ttcagatcct gggcgacaaa cacggcaaca tcatccacct cggcgagcgg
    24301 gattgttcga ttcagcgccg gcaccagaaa ctgattgaaa tcgccccttc gctggtgctg
    24361 acggcaaaat tgcgcgcgca aatgggtgaa gccgccatcg ccatcgccaa agccgtgaag
    24421 tacgacaatg ccggcacggt ggaattcctc ctcgaccacg aaggccattt ctatttcatg
    24481 gaaatgaatc cccggttgca agtcgaacat accgtcacgg agcagattac ggcgatcgac
    24541 atcgtgcgca atcaaatttc catcgcggca ggcaaaccat tggagatccg gcaaaaggat
    24601 gtcaccctgc aaggccacgc gatccaatgc cgcatcaatg ccgaagatcc gcgtaacaac
    24661 tttatgccct gcaccggcac cgtcaccgcc tacctttccc caggcggcat cggagttcgt
    24721 atcgacggcg cggtctaccg ggactatacg attccaccct attacgatgc cttactggca
    24781 aaactcaccg tgcgtggacg cacatgggag gaagccgtca gccgcatgcg gcgttcgctc
    24841 gaagaatacg tcctgcgtgg cgtgaaaacg accattccgt tcatgaagaa cgtgatgatg
    24901 gaacaggatt tccaggcggg acgattcgat acttcctacc tggagactca tccagacctg
    24961 tatcaatacg aagaatccga ggagcctgag gacctggtgt tggcgatttc cgcagcaatc
    25021 gccgcctacg aaggactctg acctctcagc caccaggcac aca
//
LOCUS       DAOQDS010000057        24668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_737_length_24668_cov_22.490066, whole genome shotgun sequence.
ACCESSION   DAOQDS010000057 DAOQDS010000000
VERSION     DAOQDS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 24668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24668
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_737_length_24668_cov_22.490066"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3354
                     /locus_tag="PK589_13800"
     CDS             <1..3354
                     /locus_tag="PK589_13800"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNJ20729.1"
                     /translation="EFGVPANQVANFLNDSGLATTNILSRVTGGTPSNIFGTIQTTGF
                     GNANLFMMNPAGMVFGPNASLNVGGSVHFTTADYLRLGDGARFNAMPGPQDAAVSSAP
                     VAAFGFLGSNPSAIVVQGSQLTLAEGNTLSLIGGNVQIANGTLSDGAIQPARLSAPGG
                     QINLVAVGSPGEALISNGAGTLAEPMLTGFTGLGTISLSHGTTIGTSGDAAGRIVIRG
                     GHFSMEDAGLEAISTSVSPNSLPSSSPRGDISIQAEEVSLSNGAHLLTSTINGTAGNI
                     ALEVGTLRSNVGPGGLPLSGAAPVTITSSSTGLGGPGSISVTGNGRAASDVVVLSNTQ
                     AVTTVPHSAQPTIVPAHIDMTAEHLELRNGTVLKTDTTGGADAGSITLKVGTLATEAG
                     PDGRVLLSSSSNCGTGCVGGQAGDITIQGIPGITPAETHLYVPIVRPDSPPTVAISYQ
                     LARNMDLRGTDIRSEAIGNAPGGQVIMRAQGSISLADTTISVATQDFQIDGPKPSGEL
                     VRNQGFSRIDILAHDVTLKDSTVRADANVSDIGSCPLCLGGPSAGEIWFRVGNSFTAD
                     NSAIINSGKGRAQAGLTKIIRDHFFSFGAIWEPDYPDYPTNSITLNHSQITVESPHSG
                     IPGYLRLRADNVTLNHSILNSQVNDVSNSRGLDGQLIDVPGAGERGRVIADGRDVQGS
                     VLVSAKTLDITGGGIIAPTTGSRIASRIELLANELTTHRGTGPGGTFDAPRILDAADP
                     TRVVLSSSSTGSGGAGRISIAGEGVAMPEGTPYRPASSIHLDGTALFTDTHSDALGGK
                     IEMISRGPIVLHDTTVSANVTDVRPASAGLQEQGGNISLSAGSLLVQGGGLSALSRGT
                     QTGGNIAITVQGPITLESGAAISASNTGSANAGSVTIHAGSEFVSRDASVTTESIHAS
                     GGNIFIQATDSIRLVNSRISTSVLGGPNSSGGNIILDPAVVTLQNSQVLAQAVQGAGG
                     NIRIIAGTFLADQTSAVSASSEFGLNGTVSIQSPVSSLSGTLATLPQSTLQTQQLVTQ
                     RCAAQGSGHLSSLVVAGRDVLPVEPGGWLMSPLSVFAVDAHDDGGRPVAETIPDAAEG
                     GNTLVELTQEHPTPEPRSNFTTPGGGCRS"
     gene            3455..5152
                     /locus_tag="PK589_13805"
     CDS             3455..5152
                     /locus_tag="PK589_13805"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNJ20730.1"
                     /translation="MTFQALAQVAPPLPPIFDPTGKSGKPPALLKEDFNTPPSEPPSS
                     ILPKVPTTPPALPDNKLGAFRVFVHDIHVTGSTVFSETELSEVTAPYRNRYLTTEDLE
                     KVRLALTLLYVNNGYLTSGAVVPDQDVTFGTIVIQMVEGKLAHIDVEGNAWFRNAYLR
                     DRVARGVSTPLNIHPLQERIQLLQQDPRIERINAELKPGDVRGESVLNVRVKDANPFR
                     AWLEFNNHQTPTVGAERGLATVAHQNVTGHGDQFMFTYGRSHGVNPIIDTSYSLPLNR
                     YETTLTAYYRRNAFLVVENPFRALDLNVDSQVIGLALRQPVYRTVTAEFALTLTGEHL
                     FLKTTSAFDAPGLPSLFIPGSSTTGVATVSALRFSQEYTHRSSSLVFAALSRVSVGLN
                     VLGATINSGPLPDGQFVSWLGQAQVVKRLDDWWGVQLLGRVAAQLASDRLFPLEQMPV
                     GGRFSVRGYRENTLVRDDAVLASFESRVPLLRFPSGEDRLQFAPFVDVGHAWNAKGHT
                     PDPHTLASVGAGLRWMILPQERARFEVYWGQQLNHVRGGEGNLQDHGIHLQLVVQVL"
     gene            5153..7495
                     /locus_tag="PK589_13810"
     CDS             5153..7495
                     /locus_tag="PK589_13810"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ20731.1"
                     /translation="MRHHRLTVFPPPSHLLRYGLKGLSLCLIIFFCCTVSLAADSQPS
                     AGDLMQRGRAAAREGSYEEALLAWKAAAELYDQAGETKNHIQALTQSAFAARTLGHIN
                     QAFLQEELALHLARQLRDSQWLVITLSELGKTYVTNHQYEVAADYIGQALEIAQAQSF
                     RALSPALQNDLGIVLALQGRPEEALAAFHQCAVSAKQEGLIPLFIRARVNAARMAIQV
                     AQFDTATSALAEAADQLAGSASSYEKAEGLVNLALGYRDLALAHSGSHASALKRSALF
                     LQEAAGMTQHPGDARLASYVYGHLGHLYEVERRFDEALQLTRQAVFTAQSARAPESLY
                     RWQWQCGRLLNRLGQLDDALSAYQEAAITLHPIRSEVTFAVQTSPDSHQPSIRALYFE
                     LADLLLRRAALMHENREAEAYLRAARDVIEAFKAAELRDYFRDDCVDQMQARITKLDA
                     LSPSTAIIYPIVFSDRTELLVSLPDGLTRHSVPVTAATLTDEVRRFRHTLEKRTTREY
                     LPHAQQLYDWLIRPLDPDLRRLQVNTLVFVPDGSLRTIPMSALHDGKEFLIEHYAVAM
                     SPGLDLTDPQPIDRAKVHLLSSGLTKAVQGFPPLPHVADELDSIRALFKGEQLIDQEF
                     LTPRLEEELKGGRYSILHIATHGQFAPDVNQSFLLTFDARLSMTQLERLVGLFRFRRE
                     PLELLTLSACQTGSGDDRAALGLAGIAVKAGARSALATLWSINDDASSELVSEFYRQL
                     HDASVSKARALQHAQLKLLSDRVYEHPAYWAAFLLLNNWL"
     gene            7544..8251
                     /locus_tag="PK589_13815"
     CDS             7544..8251
                     /locus_tag="PK589_13815"
                     /inference="COORDINATES: protein motif:HMM:NF017829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HNJ20732.1"
                     /translation="MARTFVATLLFTVSLVSTNAAAPEGVPDVPQYNAPQKMTPRARL
                     GGSLRGSEGEDPAVAALVPDHVGITVKQHPALNWFLSKHTSLPITFTLIDERSIHALV
                     ERPLAPPHQAGVHAVKLEDLGVTLQPNIQYRWYISVIKDADSPSQDIVTGGMIERCEF
                     SECSILGAMTTCTREAVMTSAAKGFWYDAMGCLCDLIDSNPADTALRKQRAALLRQVG
                     LHDVADWDLAQSHARQG"
     gene            8255..8995
                     /locus_tag="PK589_13820"
     CDS             8255..8995
                     /locus_tag="PK589_13820"
                     /inference="COORDINATES: protein motif:HMM:NF017829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HNJ20733.1"
                     /translation="MPYRRVSFPLVLMIAWLTGGYASQFPFGAPDSIAADPLVPLELR
                     LPEYKPKKADAPRARIGGHSRGTEAGAPVLIALVPDHIAFTVKNDPTLCWYQSLHTSR
                     PMVLTVVDSRGIRPVLEHSLPTPVRHGIQCLRPREYGVEFRVEESYRWYVTVVMDPNR
                     PSLDVVAGGMIERLSLDEACALGMPCPSSSCDTDGIYRYAESGLWYDAIACLVELLEQ
                     ESDKDRLRLMLDHLLHQSGVHLPGESSP"
     gene            9378..9503
                     /locus_tag="PK589_13825"
     CDS             9378..9503
                     /locus_tag="PK589_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20734.1"
                     /translation="MELVMGGLLVGVSGMLAVLIVAVWKDSASHRTGAHPSHRRV"
     gene            10109..12199
                     /locus_tag="PK589_13830"
     CDS             10109..12199
                     /locus_tag="PK589_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ20735.1"
                     /translation="MRTRTARAETIVHCPFTATQPEPALAVHPITLRWDHYTRALARG
                     LMSVCWAFAIEAHSPIPTYAAPQADAPANEELELLKEEETVSIATRHEQPISQAPSNV
                     YVITDEDIRHSGATDIPTILRRVPGLDVMQVTGADFNVSARGDNQLFANKILVMVDGR
                     SIYIDGQGLVFWKSLPVTLPEIKRIEVLKGPASVVYGFNAFDGVINIITKSAQEMKGT
                     TLQFGGGELGTISSAAVQAGSAGKLDYRLSIGHDQNQQWRNNDALAFRSNKFNAQTQY
                     NFSGDAILRVAGGFIDTNRFDGPVSNVQVPRSQFNQGYSDVHYQYRNFSLRGWWQGND
                     IPVESSTHPLLAPFVRQTNATGGSNVSFTTNTYNIEAQHSLDFGPANRLSYGVNYRYN
                     SVDGAAVSELGREDRLGFHLQDEWKILSSLTLVAGVRYDLHTRINGTWSPRVALIYNL
                     TPEHTFRVSGSVAYRPPTLFESNLDLRILPTVPPLFAPIPLFGSQRLNPEQIISYEAG
                     YQGWWLKHRLRTRLDLFFNHLSDLINFQTIGATRTLVNGGEADIYGTEAGFEFLATRW
                     LSGFANVSYQKIGQTFTGVSRRGGPDWKANVGLRGDWDNGLNGEIAVHYVAGATYPLI
                     ELFTALAPVLPAPVSPNVDSYTLLNLRGGYRFWHDKAEVAIAVYNALNDRHKEHPLGD
                     TLGSRVMGWLTLRY"
     gene            12203..14269
                     /locus_tag="PK589_13835"
     CDS             12203..14269
                     /locus_tag="PK589_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ20736.1"
                     /translation="MHAESKGVWQTMVAAFMTVSISQFAAAEPPAIRVEDSLTVPANN
                     DLELLKEEETVSIASRYEQPISQAPSNVYVVTDEDIRQSGATDLPTVLRRIPGLEVMQ
                     MTGADFNVSARGDNQPFANKMLVMVDGRSIYLDVQGNVFWKSLPVTLPEIKRIEVLKG
                     PASAMYGFNAFDGVINIITKSPEEMKGTTLQFGGGEQGTISSAAIQAGTVGKFGYRLS
                     IGRDQTQQWRDGDALAFRAHKFNVQTDYALSSTSKLQLSGGLIETNRYDGQVGEVTSN
                     SIRPSFGYANAVYEHGAFLVRAWWSGYTDHATINPLPQLAGLLSVTDRNGVSTNPFSG
                     NTYNVDVQHALDMGAANRLLYGANYRHNSLSSPAIDQFSRENRLGLFLQDEWRASSAI
                     TVVAGIRYDLHSQINGTWSPRLTLLYQPVEGHTFHLSGSAAYRPPTLFESHQDQRVTT
                     NIPTGLPFPLPSSVSSTVPVTGSTGLAPERIISYEAGYQGWYWKHRIRVRADLFYNHV
                     SDLIGSRITASGSSEFVNDPGSADIYGGEAGIEVLANRWLSGFANYAYEEIGQSFTGT
                     VRRGAPRSKVSAGVRTEWDNGLSGELSYYYVGAATYPLAQSFTQLGTIPGAGITVPGN
                     QVGSYNLLNLRAGYRFWQQKAAAGYMRDAEVAISVFNALNDEHKEHPLGDLIGRRVMG
                     WLTLRF"
     gene            14300..17422
                     /locus_tag="PK589_13840"
     CDS             14300..17422
                     /locus_tag="PK589_13840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ20737.1"
                     /translation="MAPATLTHSILVVDDDPDIALGLQDFLDHDGYQVNVAMTCAEAL
                     AQAHAHHYNAVLLDLGLPDGDGSSVLLTLHQQQPQLPVIILTAYTSTDRTVGSLTQGA
                     FAYLTKPYNRDELRAVLQRAVGVQALAVKAEHAEHALHESEARFRSLVEAATDSIVLA
                     DHNGHILSWNRAASHLFGYTDEEVRGQSLSILMPPRHRAAHERGLARVRDTGESRLIG
                     RLVELEGLRKDGSEFPLELSLAMWKSDEAVFYSGIIRDITQRRRAEEILDRLRHLHEV
                     ILTQAGEGIYGLDRDGITTFVNPTAAELLGYEPFELIDRPMHGLLHHSRSDGSPYPSG
                     ECPIYAALHDGLVHRVSADVFWRKDGHPVPVEYVSTPIIEKGRTAGAVVVFRDISERK
                     EAERAVEESQERFRQLAEHIREVFWITNPEKSHVVYISPGYEEIWGRSCDSLYAHPTS
                     WLDAIHPEDRPRIEEAAISRQALDVYNETYRILRPDGSMRWIWDRAYPIRDASNQVYR
                     IVGFAEDITEQKRIEAELIASERRYRVLFDDNPSMYFMVDAVGTVLSVNRFGAERLGY
                     EVQDLVGTSVLNVFHPEDQQAVLHNLSDCLSEVGQPKRWELRKVRKDGHVIWVRETAQ
                     AVRGHEQAPVVLIVCEDITAMKEAEIALHDSEELKNQILHSSADCIKVLDLEGRLQYM
                     NDAGQTLLEIKDLGTYTNKPWSEFWEGDDREAALAALEAAKAGDIGKFVGFCPTTTGQ
                     AKWWDVQVTPMLDTQGYSRRLLAISRDITAYKQVQESLHASEERLEHVIRGSHDGFWD
                     GHVLPDHPWYAPITPVWWSPRVREMLGYNEEEFPDILDSWMSRLHPEDREAVFERLNA
                     CMEQGVSQYDVEYRLLNKQHEYQWFHARAEVVQRDAQGQAVRMAGALQDITDRKRTEA
                     RLRLSEERLRMALTSSDLGIWDWDVTSDRLYWSEGVELLFGLAPGSFPGTYTDYIGLI
                     YLLDRGSTLTRLELSLRDQTSVHIRHRVVWPDSSIHWLAWSGRIYRNADGVATRVLGI
                     VHDTTGRMDE"
     gene            17555..18448
                     /locus_tag="PK589_13845"
     CDS             17555..18448
                     /locus_tag="PK589_13845"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ20738.1"
                     /translation="MVLLLWTLLIASPALATDVIILKSSDIAAYNQAINGFKAAIPAG
                     INLSEYDLQGDLEKGRKLARKIRASDPSLVFAVGLKAAKAAQLEIVDIPVVYSMVLDP
                     AKYGLNAQNMTGVLLEVPAERQLVMIRNLLPQLKHIGTLYDPAKTSTLIDEARRLLKQ
                     SGTELAPMQISSERDVPGSLRALLPSVDALWLVPDSTVLTDESLRFILNTALEARVPV
                     IGFSREFARSGALLCLSVNYADIGRQAGQLTRKLLEGQLSLPLKPWHPDRIETSLNLK
                     TAKFLGIDIPRELEQKADELY"
     gene            18448..20760
                     /locus_tag="PK589_13850"
     CDS             18448..20760
                     /locus_tag="PK589_13850"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ20739.1"
                     /translation="MKGPSVKDPAHLAAPPFLPNGLKRFVSLRTKFVVFFSLIIIFTC
                     SGMSWYFIHSKRVAMTERVQDLGSILVKNLAHNVRYGIILEDRVILEQFIDGVLGVDE
                     VVYAQITGADGQVLAAKSKGKLKTPLGTVRAVDQPFYPSAAIAASLIRHPLPEPTVTR
                     LRLIGESSLPFEEQDATLRVSTAGMREETFYDFALPVLKRAEPRLEALALQEEETHKT
                     RTAASTAPAFGVVQIGLTEVPMQRELAEVLRTFLLLTLGIITAGILSTNLLARRIITP
                     LRNLAAGARKVGEGDLSTFVVPTTQDEVGQLTALFNVMTKSLQERNQAISANLETIRR
                     HVTQLTTLNQSSAAITSTLDLDRLLSTVLQLLSENLGFARMVLFLWDSERGVATVGQM
                     LGMPPEAEEAARRLVMPVHEDGGFAAEILLHGKPVLVPNIETVADRISPAVLPWLRQV
                     GLSSFVAAPLRSQQRILGYLGADRGAQPCTQEDLDLLVTIASHVAVAVDNARAYTELA
                     TLTEHLERRVEERTHELQSVNERLQDHDQRRSKFVSVASHELRTPMTSIKGFVENMLD
                     GLTGQLSERQEHYLQRVKHNVDRLTRIINQLLDWSRLDVGRIDIKPESLSIDAFVRDV
                     VESFQTLAAEKSITLDVLPCEQPLVVYADRDKLEQILWNLVGNAIKFTPHAGHVSVQC
                     TAHDTHSVQITVADTGCGIAPDELPLVFNEFSKVESAMPTSQGAQLGLFITKSLVTLH
                     GGQMWVESRLGAGTQFSFTLPLSATQPAQP"
     gene            20927..23731
                     /locus_tag="PK589_13855"
     CDS             20927..23731
                     /locus_tag="PK589_13855"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ20740.1"
                     /translation="MAFAAWVLRTSTNTGCFTRQRGPTVHTAVLIVDDDRDIRESLTD
                     MLRHEGYLVESAGSGNEALSHAKQRQYGAAILDIQLPDLNGLSVLKVLMELDASLPVI
                     ILTGNATSENTIGSLAKGAFAYLTKPYNAQELKAVIRRAVSVKGLAVRAEHVEQALHA
                     SEERFRALVESATDAIVLADHYGHIIWWNGAAERMFGYSKREALGQSLAMLMPERFRS
                     AHETGIMRLTTGSAAKLIGKTVELVGLTKAGDEFPIELSLASWRAKEGAFFSGIIRNI
                     TRRKKAEESVVRLSHQNALILDAAGEGIFGLDLEGRTTFVNATAARMLEWSAAELIGK
                     PLAPLLYRLHDEGAEYVQDPLCLSAAVRDGAVHRVQNDVFTSKSGVSFPVQYVTSPIR
                     EQDRPVGVVVVFQDITARKQLECLQQAQLSISHILAQTSTLEEAVPQLLRVIGEMGPW
                     EMTIFWHKEPMNERLVCEGTWMRSSCRWDEFATICRNSEFPPGMDFPGVVWSTKLPLW
                     IPDALTDARFTRAPTAARLGIHGACLIPIRTGTVIHGVLELYADKPRLPDRQLIHILT
                     DTGMKIGQFIDRAQAGSALRATHRMTQNLLASLPGAIFLCARDFKIQYANTLAHHYFA
                     VGGASLVGRPLFECLALSEAATQQLIKEWAALNADSPHQAPERECEVASRLYRYRFFP
                     VATPESAQYQVGIVLWDVTDDKHLQDQLIQSEKLTSLGTMVSGMAHEINNPAQAILSM
                     AELIQEEKDPAVIKEFAADIVGYARHVSNVVRDFAGYARSAGRDGETEIDVAERLMEA
                     VKMARRGPHFGYVDVVTRFDAPTFLRARKSELDQVFVNLINNATQAMDGKGRLTLGAA
                     QDGAWIDVTISDTGPGIPAAVRPRIFDPFFTTKAPGKGTGLGLSIVHKIVTRHAGTIT
                     VDSTEGEGTTFRLRFPALLQ"
     gene            complement(23774..>24668)
                     /locus_tag="PK589_13860"
     CDS             complement(23774..>24668)
                     /locus_tag="PK589_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20741.1"
                     /translation="SLRPHWTDFLTILQATCFFLSGVLFIGRRCPQWNHDESGLSEGR
                     MTQACASSVTEAVERAISALDLDRLERDYWEQNEFLYIPQFLPREFVEAELASQAQVL
                     KSGLNRNYIPGHKKGGSVSYYTVREQAPQFMDLYRSDAFRAMLNQLTRVNLLLCPDND
                     PHSCALYYYTEAGDHIGYHYDTSYYKGARYTILLGLLDQSKQCRLVCDLFKDVPGKQP
                     VHMELATAPGDLVIFNGDKLWHAVTPLGEQEERIVLTLEYVTNPDMGAFKRLYSNLKD
                     SFAYFGLRSVFKRAYSSRSRA"
BASE COUNT         5265 a         7401 c         6884 g         5118 t
ORIGIN      
        1 gaattcggcg tgcccgcgaa tcaggtggcc aattttctca acgattcagg actggccacc
       61 acgaacattc tgagccgcgt caccggcggg accccttcca acatcttcgg caccatccag
      121 acgaccggct tcggcaacgc caatctgttt atgatgaatc ccgcggggat ggtcttcgga
      181 cccaatgcct ccttgaatgt cgggggctcg gtgcatttta cgacagcgga ttatctccgc
      241 ttgggggacg gggcccgctt caacgccatg ccggggccgc aggatgcagc cgtctccagc
      301 gcaccggtcg cggcattcgg ctttttagga tcaaacccct ccgccattgt ggttcaaggg
      361 agtcaactca ctctcgccga aggcaacacc ctctcactga taggcggtaa cgtccaaatc
      421 gccaacggga cattatcaga tggcgcgatc cagcccgcgc gcctctcggc accgggaggg
      481 caaatcaacc tggtcgcggt cggatcccct ggggaagcgt tgattagcaa tggagcgggg
      541 acgctcgccg agccgatgtt gaccggcttc accggactcg gaacgatttc gctttcgcac
      601 ggcacgacaa taggcacaag cggcgatgct gcagggcgca tcgtcattcg tggcggacac
      661 ttcagcatgg aggatgcagg ccttgaggcc atctcgacat ccgtctctcc aaactcgctc
      721 ccctcttcct ccccgagagg ggatatctcg attcaggcgg aagaggtttc gttgtcgaac
      781 ggcgctcacc tacttacgtc cacgatcaat ggcacggccg gtaatatcgc gctggaggtg
      841 ggtactctgc gaagcaatgt cggaccgggc ggcctcccgc tctcgggtgc cgcgccagtc
      901 actattacca gcagcagcac agggctaggc ggacccggaa gcatttcggt cacgggcaac
      961 ggacgggcgg cttcggatgt tgtagtcctc agtaacacac aggccgtgac tactgtgcct
     1021 cactccgctc aaccgacaat tgtaccagcc cacattgata tgacggcaga gcatctcgaa
     1081 ctcaggaacg ggacggtcct caagacggat accacgggag gagctgatgc cggatcgatc
     1141 acattgaaag ttggaacgct tgctacggaa gccggtcctg acggaagagt gcttctctca
     1201 agcagcagta actgcgggac cggatgtgtg gggggacagg ccggtgacat taccatccag
     1261 ggcattccag gaatcactcc ggcggaaacc cacttgtatg tccccatcgt cagacctgac
     1321 tcccctccca ccgtggccat tagttatcag ttggcgagaa atatggacct tcgaggcaca
     1381 gacattcgct ccgaagcgat cggtaatgcg ccgggcggac aagtcatcat gagggcgcag
     1441 ggatccatct cgctcgccga taccaccatc tcggtcgcga cacaggactt tcaaatcgac
     1501 ggacctaagc caagcggcga acttgtccgc aaccaagggt tcagccggat tgatatcctc
     1561 gcgcatgatg tcacgttaaa agacagcacc gttcgggcag acgcaaatgt ctcggatatc
     1621 ggctcctgcc cgctctgtct cggggggccc agcgcaggag agatttggtt tcgagtcggc
     1681 aattcattca ctgccgataa ttccgccatt attaatagtg gcaaaggcag agcccaggcc
     1741 gggctcacga aaatcatcag agaccacttc ttttcatttg gcgccatttg ggagccggat
     1801 taccccgatt atcctacgaa ctcgattacc ctcaatcatt cccaaatcac cgtcgaatct
     1861 ccgcattcag ggattcccgg atatcttcga ctccgcgccg acaacgtgac gttgaaccat
     1921 tccatcctca actctcaagt caacgacgtg tcgaactcgc gagggctgga cgggcaattg
     1981 atcgatgtcc ccggcgccgg cgaacgcgga cgagtgattg cagacggccg ggacgtacag
     2041 ggcagcgtct tggtttcggc aaagacgctc gacatcaccg gcggcggaat tatcgcgcca
     2101 accacaggca gccgcatcgc gagtcgcatc gagttgctcg cgaacgagtt gacgacccac
     2161 aggggaactg ggccgggagg aacgtttgat gccccacgca tcttagatgc cgccgacccc
     2221 acgcgcgtgg ttctgtcgag cagcagcacg ggaagcggag gcgcaggcag gatcagcatc
     2281 gcaggcgagg gcgtggctat gcccgagggc accccatacc ggcccgcgtc gtccattcat
     2341 ctcgatggca cggccctctt caccgatacg cacagcgatg ccctgggagg caaaattgag
     2401 atgatctcgc gcggcccaat cgtcctgcat gacaccaccg tctccgccaa tgtcacagat
     2461 gtgcggccgg cttctgccgg cttgcaagag caaggtggga acatcagtct ctcggcaggg
     2521 agtctcttgg tacaaggtgg cgggctttcg gcactctctc gcggcacaca gaccggcggc
     2581 aacatcgcga tcacggtgca aggcccgatc acgctggaat ccggtgcggc aatttcggcc
     2641 agtaacacgg ggtcggcaaa tgccggcagc gtcaccatcc acgccgggtc agagtttgtg
     2701 agccgggatg cttccgtgac gacggaatcc attcacgcaa gcggtggcaa catcttcatt
     2761 caagccaccg attccatacg actcgtgaat agccgcatca gtacctctgt gctaggtggt
     2821 ccgaattcat ccggaggcaa tattattctt gatccggccg tggtcacact gcagaacagt
     2881 caggtactcg cgcaagcggt gcagggggca ggcggcaata ttcgtatcat cgcagggaca
     2941 ttcctggccg atcagaccag cgccgtcagt gcgtcgtccg aattcgggtt gaacggcacc
     3001 gtctccatcc aatcgccggt ctccagcttg agcgggacgc tcgcgacgct cccgcaaagc
     3061 accctgcaaa cacagcaact tgtcacacaa cgctgtgccg cccagggaag tggtcatctc
     3121 agcagtctcg tcgtcgcggg acgcgatgtg ctgcccgtcg aaccaggcgg atggttgatg
     3181 agcccgttaa gcgtcttcgc tgtggatgcc catgatgacg gaggtcggcc tgtcgctgag
     3241 acgattcctg atgcagccga agggggcaac accctggtgg aactgacgca ggaacatccg
     3301 actcctgagc cgcgctcgaa cttcactacc ccgggaggag gctgtcgctc ataacgggtt
     3361 gagccggcgc gccggcggac aggtacaaca cttggctttg cgcatcgtta cggtcccatg
     3421 tgtatgtcgc gttgtggtac tgagcctttg ccttatgaca ttccaagccc tggctcaagt
     3481 ggcgcctccc ctcccgccga tttttgaccc gaccgggaaa tcggggaaac cgccggccct
     3541 gctcaaagaa gacttcaata ctcctccctc ggagccaccc agctcgatac tccccaaagt
     3601 accgactacg cctcccgcgc tgcccgacaa taagttgggg gcatttcgag tgttcgtgca
     3661 tgatatccat gtgaccggca gcaccgtatt ttctgaaact gaactttcag aagtgaccgc
     3721 gccgtatcgc aatcgatacc tcacaacaga agatttggag aaggtgcgac tcgccctgac
     3781 acttttatat gtgaacaacg gctatctcac atccggcgca gtggttccgg accaggacgt
     3841 gacgttcggc acgattgtca ttcagatggt cgaggggaaa cttgcgcaca tcgacgtcga
     3901 ggggaatgcg tggtttcgaa atgcgtatct ccgcgaccgt gtggcgcgcg gggtgtcgac
     3961 gccgctcaac atccacccgc ttcaggagcg gatacaactg ctgcaacaag accctcgcat
     4021 cgaacggatc aatgcagaac tgaaacctgg agacgttcgc ggcgagagcg tcttgaacgt
     4081 gcgcgtgaaa gatgccaatc cctttcgggc ctggctggag ttcaataacc atcaaacccc
     4141 gacggtgggc gccgaacgcg gtctggcgac cgtggcgcat cagaacgtga ccggtcacgg
     4201 cgaccaattc atgtttacct acggccgctc ccacggcgtc aatcccatca tcgatacgtc
     4261 ctatagccta ccgctcaaca ggtatgagac gacgctgact gcctactatc gacgcaatgc
     4321 ctttctcgtc gtcgaaaatc cttttcgcgc cttggatttg aacgtggact cccaggtgat
     4381 cgggctcgct ctgcgacagc cggtctatcg aaccgtcacc gctgagttcg cactgacgtt
     4441 gacgggggag catctatttt tgaagaccac atccgctttc gatgctcccg gccttccctc
     4501 gctgttcatt cccggttcgt ccacgacagg agtggccacc gtcagcgcac tgcgattcag
     4561 ccaggagtac acacatcgct cttcatcctt ggtatttgct gccctatccc gcgtcagtgt
     4621 cgggttgaat gtattgggcg ccacgatcaa tagcggacca cttcctgatg ggcagttcgt
     4681 gtcctggttg ggccaggctc aggtcgtaaa gcgattggat gactggtggg gcgtgcaatt
     4741 gttggggcgt gtggcagcgc agttggcaag tgaccgactc ttccccctgg aacaaatgcc
     4801 ggtcgggggc cgtttcagcg tgcgcggata tcgcgagaac acgcttgtgc gggacgatgc
     4861 cgtgctggct tcattcgagt cgcgagtgcc gttgctccgg ttcccatccg gagaagaccg
     4921 gctgcaattc gccccgttcg tcgatgtcgg ccacgcctgg aacgccaaag gccacacccc
     4981 agatccgcat acgttggcga gtgtcggagc tggactccgt tggatgatcc tccctcagga
     5041 gcgggcccgc ttcgaggtgt attgggggca gcaactcaat cacgtccgcg gcggggaagg
     5101 caatttgcaa gatcatggca ttcatctcca acttgttgtg caggtgctgt aaatgaggca
     5161 tcaccggctc acagtattcc cgccgccctc gcatctgctt cgatacggac tcaaggggct
     5221 cagtctgtgc ctgattatct tcttctgctg taccgtttct ctggctgcgg acagtcagcc
     5281 ttcggctggt gacttgatgc agcgcggtcg agcggcggcc cgagaaggat cctatgaaga
     5341 agcgctcctc gcatggaagg cagccgcgga gttgtacgac caggccggtg agacgaagaa
     5401 ccatatccaa gcgctgacac agtccgcgtt tgccgctcgt accttggggc atatcaacca
     5461 ggcgtttctc caagaggaac tcgcgctgca cctcgcacgc cagctgcgcg attctcaatg
     5521 gctggtcatc acgctctccg aattaggcaa gacctatgtc acgaaccatc agtatgaggt
     5581 ggcagcggac tatattggtc aagcacttga aatcgcacag gcacagtcat tccgggcgct
     5641 gagtcccgca ctccagaatg atctcgggat cgttttggcc ttgcagggac gcccggaaga
     5701 agccctggcg gcattccacc aatgtgcagt gagtgcgaaa caagaggggc tcatcccgct
     5761 cttcattcgt gcccgtgtca acgcggcccg gatggcgatt caggttgcgc agttcgatac
     5821 agccacatcg gccctggccg aagctgcaga tcaacttgcc ggctccgcct cctcctacga
     5881 aaaggccgaa ggactcgtga atctggcttt gggatatcgc gatctggcct tggctcattc
     5941 tggctctcac gcgtccgccc tcaagcgatc tgcgctcttc ttgcaggaag ccgcagggat
     6001 gacacaacac ccgggcgacg cgcgcctcgc atcatacgtt tacggccatc tcgggcacct
     6061 ctatgaagtt gaacgccggt ttgatgaagc ccttcagttg actcgacaag cggtcttcac
     6121 cgcgcagtcg gctcgcgcac cggagtcgtt gtatcggtgg caatggcaat gcgggcggct
     6181 gctgaatcgt ctcggccaac tcgacgacgc cctgtcggcc tatcaagaag ccgccatcac
     6241 tctgcatccc attcgaagcg aggtaacatt cgccgtccag actagcccgg attcgcatca
     6301 gccatccatc cgcgcgttat attttgagtt agccgacttg cttctgcggc gagccgcgtt
     6361 gatgcatgaa aaccgagagg ccgaggcgta cttgcgtgcg gcccgggatg tgattgaagc
     6421 ctttaaggct gcagaacttc gagactactt tcgagacgac tgcgtcgatc agatgcaggc
     6481 ccgcatcaca aaactggacg cgctctcacc ttccactgcc attatttatc ctatcgtctt
     6541 cagcgatcgc accgagctcc tcgtcagtct tcctgacggg ctgacacgtc attccgttcc
     6601 ggtgacggcc gccaccttga cggatgaagt gcgtcgcttc cggcatacgc tcgaaaagcg
     6661 gacgactcgt gaatacctcc ctcacgcgca gcagctgtac gattggctga tccgacctct
     6721 tgatcctgat cttcgccggc tccaagtaaa cacattggtc tttgtcccgg acggatcgct
     6781 ccgcaccatt cccatgtcgg ccctgcatga cggaaaggag ttcctcatcg agcactatgc
     6841 cgtggccatg tcccccggcc ttgatttgac ggaccctcaa ccgattgatc gtgccaaggt
     6901 gcatctgttg tccagcgggc tgaccaaagc cgtgcagggg tttcctccat tgccccatgt
     6961 cgccgacgag ctcgattcca ttcgtgcgct gtttaaggga gagcaactca tcgaccagga
     7021 gtttctgacg ccccggttgg aagaggaatt gaaggggggg cgttacagca tccttcacat
     7081 cgccactcat ggacagtttg ccccggacgt caatcagtcg ttcctcctga ccttcgatgc
     7141 ccgtctgagc atgacccagc tggagcgact cgtcggcctg tttcgttttc gacgagagcc
     7201 gcttgaattg ctcaccttga gcgcctgtca gaccggcagt ggtgacgatc gggcagcgct
     7261 tggactggcc gggatcgccg tcaaggcagg tgccagaagt gccctcgcca cactctggtc
     7321 catcaatgac gacgcctcgt ccgaactcgt ctcagaattt taccgtcaac tgcatgacgc
     7381 atcggtctcc aaggcgcgag ctttgcagca tgcgcagttg aagttgctgt cagatcgggt
     7441 gtatgaacat cccgcctatt gggcggcatt tttattattg aacaattggt tgtgacggac
     7501 tctggaacag ggtgatcgct tggcgacgtg gctctaccgc tccatggctc gaacattcgt
     7561 agccacactc cttttcaccg tgtcgttggt gagtaccaat gcggccgcgc cggaaggtgt
     7621 gccggacgtc ccccagtaca atgcacccca gaagatgacg ccccgggcac gtctgggcgg
     7681 aagcttacgc ggcagtgagg gtgaagatcc tgccgtcgcg gcgcttgtcc cggaccacgt
     7741 cggcatcacc gtcaaacagc atccggcatt aaactggttc ttgtccaagc atacgtcatt
     7801 gccgatcact tttaccctga tcgatgagcg ctccattcat gccttggttg aacgccccct
     7861 cgcgccgcct catcaagccg gtgttcacgc cgtcaagctc gaagacctgg gggtgacact
     7921 tcaaccgaac attcagtacc gctggtatat atccgtgatc aaagatgccg actcgccctc
     7981 acaggatatc gtcacgggtg gaatgatcga acgctgcgaa ttcagcgagt gctcgatcct
     8041 aggcgccatg acgacatgta cgcgcgaagc cgtcatgacc agcgccgcca aaggattctg
     8101 gtacgacgcg atgggatgcc tctgcgatct cattgactcc aatccggcag ataccgcgct
     8161 ccggaagcag cgtgccgcct tgctcaggca agtgggcctg catgatgtgg cggactggga
     8221 tctcgcacaa tcccatgcgc gacaagggtg acgcatgcca taccggcgag tcagtttccc
     8281 actggtcttg atgatcgcgt ggctgaccgg ggggtatgcg tcgcagtttc cgttcggcgc
     8341 ccctgatagt atcgcggcgg atcctctcgt ccctcttgaa ttgcgcctgc ccgaatacaa
     8401 accgaaaaag gcagatgccc ctcgagcccg catcggggga cacagccgtg gaacagaagc
     8461 cggagcccct gtcctcattg ctctggttcc ggatcacatt gcctttacag tcaagaatga
     8521 tcccaccctc tgttggtacc agtctttgca tacctctcgg ccaatggttc tgacggtggt
     8581 cgattcccgc ggtatccgcc ccgtcctgga gcattcactg cccacgccag ttcgtcacgg
     8641 aatacagtgc ctgaggccgc gcgagtatgg ggtcgaattc agagtagaag agtcgtatcg
     8701 atggtatgtg acggtggtga tggaccccaa cagaccctcg ctcgacgtcg tcgcaggcgg
     8761 aatgatcgaa cgactctctc tcgatgaagc ctgcgctctc ggcatgccct gcccctcctc
     8821 ctcttgcgat accgacggga tttatcgcta tgcggagtca ggtctctggt acgacgccat
     8881 tgcctgtctg gtggaactgc tcgagcagga gtctgacaag gataggctgc ggctgatgct
     8941 ggaccatttg ctgcaccaat ccggtgtcca cctgccggga gagtcgtcac cgtaataaca
     9001 acgttgagac tcgcactcac cggctctctc cattcttcac tgccactgct tctagccgtc
     9061 taccgatcag cgcgcctccc cctaaaaaca tgtagcccgc tgatcgttgt ccgttatttg
     9121 attttctcgg ttgaagacca ccgtgattgg gtatctcatc agatacgcta ggatcggatc
     9181 cgatacagtg agacgggcaa ccgctcaggg atacgactgt tacctcactc gactttccaa
     9241 acattttttc catcgagccg gagctgtgcg taccgatcgt tgtggcacgg ctgttgctgt
     9301 acctttggac atgtagacga ttctttgggc ggttgtagaa agacacagcc ccccttcgaa
     9361 atagaaagga gcctgacatg gaactcgtca tgggcggtct cctggtgggc gtcagtggca
     9421 tgttggccgt cttgatcgtc gcggtctgga aagacagcgc ctctcatcgt acgggggcgc
     9481 atccgtcaca ccggagggtt tagccgcttg atgttcgttt gaacgaagcg gcgcggcaga
     9541 aaaacacagc cagaacggat cgggaggcgt acacctccat acgcctctcg tgcaaaggag
     9601 ttgcaccatg aaccggttcg tgtatttgag acctcaaaag cgccagcgca tgactgtcag
     9661 cctcccggcg gatcttttag aacggatgcg cgatgctgcg tactggacat ccgggaccac
     9721 catggccggc ctcatttcgt cggccattga agatctgctc cacaatctgg aatcgcaaaa
     9781 tggccgaccg ttctccccgc gcctccagga cctaaagcca ggccgcccca ggtccaataa
     9841 accggatcaa tcgtctgtat ctgaaaacat acagtctgta tcccgttaga tacgcacagg
     9901 ctcgccacga ttccccgcgc caccttcctt cgtgccacgt tcatccgaat caatcggatc
     9961 ttttcggaat ctcaacccgc cgtttctttc tctatccgca cgaaacaagc atgccttttg
    10021 cgagagaatt tgttgagtca aagtgggttc actccttttc tccgtcagac aacgtccgac
    10081 tccgccgcta aagggctcaa ggtatgccat gcgcactcgg accgcacgag ccgaaaccat
    10141 cgtccattgt ccatttactg ccacgcaacc tgagcccgca ttggcggtac acccaatcac
    10201 gcttcgctgg gatcactata cgcgcgcatt ggcgagaggt ctcatgagcg tatgctgggc
    10261 cttcgccatc gaggcacatt cgccgatccc cacctatgct gcaccacaag cagacgcccc
    10321 ggcaaacgaa gagctggaac tactcaaaga agaagaaacg gttagcatcg ccacgcgtca
    10381 cgagcaaccg atttcacaag cgccttcaaa cgtatacgtc attacggatg aagacattcg
    10441 gcattccggc gccaccgata ttcccaccat tctccggcgc gtgccggggc tggatgtcat
    10501 gcaagtcacc ggggctgact tcaacgtcag cgcacgaggc gacaaccagc tcttcgcgaa
    10561 taagattctc gtcatggtgg acgggcgatc gatctacatc gacggccagg ggctcgtgtt
    10621 ctggaaatca ctgccggtga cgctgccgga gatcaaacgc attgaggttc tcaaggggcc
    10681 ggcatccgtg gtctatggat tcaatgcctt cgacggggtc atcaacatca ttaccaagtc
    10741 cgcgcaggag atgaagggta cgacactgca gttcggtggt ggggaactcg gcaccatttc
    10801 cagcgctgcg gtacaggccg ggtcggcagg caaactcgat tatcgcctat ccatcgggca
    10861 cgaccagaat cagcaatggc gaaacaatga tgcgctggcg ttccgatcga ataaattcaa
    10921 cgcacagaca caatacaact tctcgggcga cgccatcttg cgcgtcgctg gcgggttcat
    10981 tgatacaaac aggtttgacg gtcccgtctc aaacgtgcaa gtccctcgct cccaattcaa
    11041 tcaaggctat tccgacgtcc actaccaata tcggaacttc tctcttcgcg gctggtggca
    11101 gggcaatgac atccccgtgg aatcttccac ccatccgctc ctcgccccat ttgtccggca
    11161 gaccaatgcg acgggtggct cgaatgtctc gttcaccacg aatacctata atatcgaggc
    11221 gcaacacagt ctcgacttcg gcccggccaa ccggctgagt tacggcgtca actaccgcta
    11281 taactccgtc gacggcgcag ccgtctcaga attgggccgt gaagatcgac tgggattcca
    11341 tctgcaggat gagtggaaaa tcctgtcatc gcttactctt gtggcgggag tccgatacga
    11401 cctgcatacc cgaatcaacg gcacctggag cccgcgcgtt gcccttatct acaatctgac
    11461 tccggagcat acgttccgtg tcagcggatc agtggcatac cggccaccta cgctgttcga
    11521 gtccaatctg gacctgcgaa tcttaccgac cgtccctccc ctgttcgccc ccattccgct
    11581 cttcggttca cagaggctca atccggaaca gattatttcc tatgaagccg gctaccaggg
    11641 gtggtggcta aaacaccggc ttcggaccag gctcgatctg ttttttaacc atctctcaga
    11701 cctgatcaat tttcaaacca tcggtgccac acgcacgttg gtgaacggcg gggaagcgga
    11761 catctacgga accgaggctg gatttgaatt tctggcgacg agatggttga gcggctttgc
    11821 caacgtgtct taccaaaaga tcgggcagac ctttaccggc gtctcacgtc gaggcggacc
    11881 agactggaaa gccaatgtgg ggttgcgtgg cgactgggac aatgggctca atggtgaaat
    11941 tgcggtgcat tacgtcgcag gcgccaccta ccccctgatt gaattgttta cggccctcgc
    12001 tccggtcctg cccgcgccgg ttagccccaa cgtcgatagc tataccttgc tgaacctccg
    12061 cggcgggtac cgattctggc atgacaaagc cgaggtggcc attgccgtct ataacgcgct
    12121 gaacgaccgc cataaggaac atccgttggg agacacgctg ggaagccgag tcatgggttg
    12181 gctcacctta cggtattgag ttatgcatgc tgaatccaag ggagtatggc agacgatggt
    12241 cgcggcattc atgacagtga gcatcagtca gttcgctgcc gccgaacctc cggccatccg
    12301 ggtcgaggat tccctgaccg ttcccgccaa taacgatctg gagttgttga aagaagagga
    12361 aaccgtgagc attgcatcac ggtacgaaca acccatttcg caggcaccgt caaacgtcta
    12421 cgtcgtcacg gatgaggaca tccggcagtc cggcgccacc gatctcccaa ccgtgctccg
    12481 tcgcatcccg ggtttggaag tcatgcaaat gaccggcgcc gatttcaacg tcagcgcgcg
    12541 aggtgacaat cagcccttcg caaacaagat gctggtcatg gtcgacggac gttcgatcta
    12601 cctcgatgtg caaggcaacg tcttctggaa atcccttccc gttacactcc cggagatcaa
    12661 gcgcatcgaa gtgctgaaag gccccgcgtc ggccatgtat ggatttaatg ccttcgatgg
    12721 ggtcatcaac atcatcacca agtcaccaga agagatgaaa ggcaccaccc ttcaattcgg
    12781 cggaggggag caaggcacca tcagcagcgc ggccatccag gcgggtacag tcggcaagtt
    12841 cggctatcga ctctctatcg gtcgcgatca aactcagcaa tggagagacg gcgacgcgct
    12901 tgcgtttcgc gcgcacaaat tcaatgtcca gacggactat gcgttgtcct cgacgtcgaa
    12961 gctgcaactc tctggagggc taatcgaaac gaatcggtac gacgggcaag tcggtgaagt
    13021 caccagcaat tccattcgcc cttcctttgg gtacgccaat gcggtctacg agcacggcgc
    13081 ctttctcgtt cgtgcatggt ggtccggata tacagatcat gccaccatca accctcttcc
    13141 ccaattggcc ggccttctca gcgtgaccga tcgtaacggc gtatcgacca atccgttttc
    13201 aggcaatacg tacaatgtgg atgtgcaaca tgcattggat atgggtgcgg ccaatcgcct
    13261 cctctatggt gcgaactacc ggcacaattc gctttcgagt ccggccatcg atcaattcag
    13321 cagggaaaac cggctcgggt tgttcttgca ggacgaatgg cgggcatcct ccgcgattac
    13381 cgtcgtcgcc ggtatccggt atgatctcca cagccagatc aacggcacct ggagtcctcg
    13441 cctcaccctg ttgtatcaac cggtagaggg gcacactttt catctctcgg gatcggcggc
    13501 ctatcgtcct cccacgctct tcgaatccca tcaggaccaa cgagtcacga cgaatatccc
    13561 gaccggttta cccttcccat taccttcgtc agtctcttca accgtaccgg tgacgggctc
    13621 gaccggtctc gctcctgaac ggatcatttc gtatgaggcc gggtatcagg gttggtactg
    13681 gaaacatcgc atacgcgtac gggcggatct attttataac cacgtctcgg acctcatcgg
    13741 cagccgaatc accgcgagcg ggtcttctga gtttgtgaac gatcccggat ccgccgacat
    13801 ctatgggggc gaagccggca tcgaggttct ggccaatcgc tggttaagcg gattcgccaa
    13861 ttatgcatat gaggagatcg gccagtcctt caccggaaca gtccgccggg gcgcgccacg
    13921 atcgaaggtg agcgcagggg tccgcaccga atgggataac ggcctgagcg gagagctcag
    13981 ttattactac gtgggcgcgg ccacttatcc gcttgcacaa agcttcaccc aactcggcac
    14041 tattccggga gctggcatca cggtgcctgg aaaccaggtg ggcagctata acctcctcaa
    14101 tctgcgtgcc ggatatcgat tctggcaaca gaaggcggcg gccgggtata tgcgtgatgc
    14161 ggaggtggcg atctcggtct tcaacgcgct caatgacgaa cacaaggaac accccctggg
    14221 cgacttgatc ggccggcggg tgatgggttg gctgacgctt cggttttaac ctgtgcccga
    14281 cgacactcgg gaggatgcaa tggcaccggc aacactgact cattccatcc tggttgtgga
    14341 cgacgatcct gatattgcgc ttgggcttca ggatttcctc gaccacgacg ggtatcaagt
    14401 gaacgtcgcc atgacctgcg cggaagcgct cgcccaagcc cacgcccatc actacaatgc
    14461 ggtattactc gatctgggcc tgccggacgg agatggctct tccgttctgc tcacgctcca
    14521 ccaacaacag ccgcaactgc cggtgatcat ccttacagct tataccagca ccgaccgcac
    14581 agtcggctcg ctgacacaag gtgccttcgc ctacctgaca aagccctata atcgagatga
    14641 actacgcgcg gtcctgcaac gggcagtggg cgtccaggcc ctggccgtca aggctgaaca
    14701 tgccgagcat gccctgcatg agagcgaagc acggtttcgt tccctcgtcg aagcggcgac
    14761 cgactccatc gtcctcgccg atcacaatgg tcatattctt tcctggaatc gcgcggcttc
    14821 gcacctgttc ggatacacgg atgaggaagt ccgcggacag tccctcagca tcctgatgcc
    14881 accccggcat cgcgccgctc acgaacgggg gctggcgcgg gtccgagaca cgggggaatc
    14941 ccgcctcatc ggccgcctcg tcgaactcga aggattgcgg aaggacggga gcgagtttcc
    15001 tctggaactc tcactggcca tgtggaaaag cgacgaagcc gtgttttaca gcggcatcat
    15061 ccgcgacatt acacaacgac gccgcgccga ggaaattctt gaccgcctgc ggcatctcca
    15121 cgaggtcatc ctcactcagg ccggcgaagg catctatggc ctggatcgcg acggaatcac
    15181 gacgtttgtg aatccgacag ccgccgaact actcggctac gaaccgttcg aactgatcga
    15241 ccggccgatg catggcctgc ttcatcattc tcgatccgac ggcagcccct atccgtcagg
    15301 tgagtgcccc atctatgccg cattgcacga cggactggtg catcgcgtct cggccgacgt
    15361 tttctggcga aaggacggtc accccgtgcc tgtggaatac gtcagtacgc cgatcatcga
    15421 aaagggacgc acagcaggcg cggtcgtggt gtttcgcgat atctccgaac gcaaagaggc
    15481 tgagcgcgca gtcgaggaaa gccaggagcg gtttcgtcaa ctggcggaac atatccgaga
    15541 ggtgttctgg atcacgaatc cagaaaaatc gcatgttgtg tatatcagcc ccgggtatga
    15601 ggaaatctgg ggtcgctcct gcgatagtct ttacgcccat cccacgtcct ggctggatgc
    15661 cattcaccct gaagatcggc ctcgaatcga agaagccgcc ataagccgac aagcgttgga
    15721 cgtgtacaac gaaacctacc gcatcctgcg tccggacgga tccatgcgat ggatctggga
    15781 tcgcgcctat ccgatccgtg acgcctcgaa tcaggtctat cgcatcgtgg ggttcgctga
    15841 ggatattacc gagcagaaac ggatcgaggc ggaattgatc gctagcgaac gacgctatcg
    15901 ggtcctcttc gacgacaacc cgtctatgta tttcatggtc gatgccgttg gcaccgtgct
    15961 ctccgtcaat cgcttcggag ccgaacggtt gggctacgag gtgcaggatc tcgtcgggac
    16021 ctccgtcctc aacgtgttcc atccggagga tcaacaggcc gttttacaca atctgtccga
    16081 ctgcctctcc gaagtgggac aaccgaagcg ctgggaactt cgcaaagtcc gaaaagatgg
    16141 ccacgtgatc tgggtgagag aaaccgccca ggccgtgcgg ggacacgaac aagcccccgt
    16201 ggtgctgatt gtctgcgagg acatcaccgc catgaaagag gctgaaatcg cgcttcacga
    16261 cagtgaggag ttaaagaacc agatcctgca cagcagcgcg gactgcatca aggttctcga
    16321 cctggaaggg cggctgcaat acatgaatga tgccggacag acgttgctgg agatcaagga
    16381 tcttggcacc tacaccaaca aaccctggtc ggaattttgg gagggagacg atcgggaggc
    16441 tgccttagcc gcgctggagg cagccaaggc cggggacatt ggaaagttcg tcgggttctg
    16501 ccctaccacg actggacagg cgaaatggtg ggatgtgcag gtcactccta tgcttgacac
    16561 acaaggctat tcccggcgtc tcctggcgat ttctcgcgat attactgcgt ataaacaggt
    16621 ccaagaatcg ctccacgcca gcgaggaacg gttggaacac gtcattcgcg gctcccacga
    16681 cgggttctgg gacggccatg tcttgcccga tcatccttgg tacgcgccta ttacccccgt
    16741 gtggtggtct ccccgcgtgc gcgaaatgtt gggatacaat gaagaagagt ttccggacat
    16801 tctcgacagc tggatgtccc gattgcaccc tgaagatcgt gaagcagtct ttgagcggct
    16861 gaatgcctgc atggaacaag gcgtctccca gtacgacgtc gagtaccggc tcttgaacaa
    16921 gcaacatgag tatcaatggt ttcatgcacg cgcggaggtg gtccagcgcg atgcacaagg
    16981 acaggccgtc aggatggccg gcgcattgca agatatcacc gatcgcaaac gaaccgaggc
    17041 ccgactgcgg ctcagcgaag agcgcttgcg catggccctg acctcatcgg atctcggcat
    17101 ctgggactgg gatgtgacct ccgaccgtct gtattggtca gaaggcgtcg aactgctatt
    17161 cggcttggcg ccagggtcct ttcccggcac ctataccgac tatattggat tgatctattt
    17221 gttagatcgc ggatctactc ttactcgcct cgaactcagt cttcgtgatc agacttccgt
    17281 ccatattcgg catcgcgtcg tatggcccga tagctcgata cattggctgg catggagcgg
    17341 acggatctat cggaatgccg acggagtcgc aacgcgtgta ctcggcatcg tgcacgatac
    17401 gacaggacgc atggacgaat gacctggcga cattcggcga cctcataccg ggcaggctga
    17461 ttcgacgagg acgcagattg tgacgtgtgc attcctacaa cggacgtgtg acagcagggc
    17521 atcaggacgc ctttggaaca ggctatgctg gagcatggtt ctcctcttgt ggaccttgct
    17581 gatcgccagt cctgccctgg caactgacgt catcattctc aaatcatctg atatcgccgc
    17641 ctacaatcaa gccatcaatg gcttcaaagc cgctatcccg gccggaatca atctctccga
    17701 atacgactta cagggagacc ttgagaaggg tcggaaattg gctcgaaaaa tccgcgcatc
    17761 cgatccgtca ttggtcttcg ccgtcggatt gaaggcggcc aaggcggccc aacttgaaat
    17821 cgtcgacatc cccgtcgtgt actctatggt attggatccg gccaaatacg gtctcaacgc
    17881 tcagaatatg accggggtcc tgctggaagt tcccgcagaa cggcagctag tcatgatccg
    17941 caacctactg cctcaactca aacacatcgg cacgctgtat gacccagcca agacctcgac
    18001 gctgattgac gaagccagac gtctactgaa acagagcgga accgaactgg ctccgatgca
    18061 aatcagcagt gaacgggatg tgcccggaag cttgcgtgcc ttactgcctt cggtcgacgc
    18121 gctatggctc gttcccgatt ccacggtgct gacggacgag tctctgcgct ttatcttaaa
    18181 tacggcactg gaagctcggg taccggtgat tggcttctca cgcgagttcg cgcggagcgg
    18241 ggcgttattg tgcctgtccg tcaactacgc cgacatcgga cggcaggccg gacaactcac
    18301 acgcaagctg ctcgagggcc agctctcgtt accgctaaaa ccctggcacc ccgaccgcat
    18361 cgaaaccagc ttgaatctca aaacggccaa attcctcggt atcgacattc ctcgtgaact
    18421 cgagcaaaag gccgacgagc tgtattgatg aaaggaccgt ctgtgaaaga tcccgcccat
    18481 cttgccgccc cgccgttcct cccgaacggg ctgaaacgat tcgtcagcct gcgaaccaag
    18541 ttcgtcgtgt tttttagtct gatcatcatc ttcacgtgct caggtatgag ttggtatttc
    18601 atccatagca aacgcgtcgc gatgaccgaa cgggtccagg acctcggcag catcctggtg
    18661 aagaacctcg ctcacaatgt gcggtacggc atcattctcg aagatcgggt cattctggaa
    18721 caattcatcg acggggtgct cggagtggat gaagtcgtct acgcgcagat caccggcgcc
    18781 gatggccagg tccttgccgc gaagagcaaa ggcaaattga agacaccgct cgggacggtg
    18841 cgcgccgtcg atcagccgtt ttacccgtcc gctgcgatcg cggcatccct gatcagacat
    18901 ccgctcccgg aaccgactgt gacacggctg cgactcattg gcgaaagtag cctccccttt
    18961 gaagagcaag acgccacgct tcgcgtatca acggccggca tgcgagaaga aacgttttac
    19021 gattttgcgc ttcccgtgct caaacgcgct gaaccgagac tggaagcact ggcgcttcaa
    19081 gaggaagaga cgcacaagac ccgcaccgcg gcgtcgacgg cgcctgcctt tggcgtcgtc
    19141 caaatcggcc tcactgaggt acccatgcag cgcgaactgg cggaggtgct acggaccttc
    19201 ctcctcctca ctttgggcat tattacggcc ggtattctct ccaccaatct tcttgcccgc
    19261 cgcatcatta cgcccttgcg caatctcgcg gcgggagccc gcaaagtcgg cgaaggcgat
    19321 ctctccacct ttgtcgtgcc gaccacacaa gacgaagttg gccagctcac tgcgctcttc
    19381 aatgtgatga ccaagtcgct tcaggaacgt aatcaggcga tctctgcaaa cctcgaaacc
    19441 atccgccgcc acgtcaccca gttgacgacc ctgaaccaat ccagcgcggc gattacctcc
    19501 accctcgatc tggatcggtt gctctctacg gtgctgcagc ttctgagcga aaatctcggc
    19561 ttcgccagaa tggtcctgtt cttgtgggat agcgagcgcg gtgtcgccac ggtaggacaa
    19621 atgctgggca tgccacccga ggctgaagaa gccgcacgtc gtctggtcat gcccgtgcat
    19681 gaggacggcg ggttcgccgc ggaaatcctc ctccacggga aacccgtcct ggtgccgaat
    19741 atcgagaccg tcgccgaccg catctctccc gccgtcttgc cttggcttcg ccaggtcgga
    19801 ttgtcgtcat ttgtcgccgc ccctctccgc agtcagcaac gcattctcgg atatctcggg
    19861 gcggatcgcg gcgcacagcc ttgcacccag gaagatctcg atctgctggt caccatcgcc
    19921 agccatgtgg cggtcgcggt ggacaacgcg cgggcgtata ccgaactggc caccttgacg
    19981 gaacatctcg aacgacgcgt ggaggaacgg acgcatgaat tgcaatccgt caacgagcgc
    20041 cttcaggacc acgatcagcg acgctcgaag tttgtttccg ttgcatctca tgaattgcgt
    20101 acaccgatga cgtccatcaa agggttcgtc gaaaacatgc tggatggatt gaccggacaa
    20161 ctgtccgaac ggcaagagca ctatctgcaa cgcgtgaaac ataacgtgga tcggctgacg
    20221 aggatcatca atcaattgct ggactggtcc cgcctggatg tcggacgcat cgacatcaaa
    20281 cccgagtcgt tgtctatcga tgcgtttgtc cgtgatgtcg tggagagctt tcagaccctg
    20341 gcggcagaaa agtccattac gttagacgtc ctgccgtgtg agcaacccct tgtcgtctat
    20401 gctgatcgag acaagctgga acagattttg tggaatctcg tcggcaacgc catcaagttc
    20461 actccgcatg ccggtcacgt ctccgtgcaa tgcactgccc atgatacaca ttcggtccaa
    20521 atcaccgttg ccgacaccgg gtgtggaatt gcccccgacg agttaccgct ggtgtttaac
    20581 gaattttcca aagtggaatc ggccatgccg acgtcgcaag gcgcacagct cggcctcttt
    20641 attacgaaaa gcctcgtcac cctgcacggc ggtcaaatgt gggtagaaag ccgattgggg
    20701 gcgggaacgc agttctcctt caccttgccg ctctccgcca cgcaaccggc gcaaccgtag
    20761 attcacgctt cactcgaagc gacgtctctt ccctgtatcc gttccgataa tagccgcatt
    20821 gttttgaaca gccctgactg gactgttcag aatgattcat ctgtcggctt gtcttgcttc
    20881 gccggagaat caatgagttg ttcgtgcctc tcaggagcgt atcagcatgg cgtttgcggc
    20941 atgggtattg cggacatcaa ccaacacggg atgtttcacc agacagcgtg gccccaccgt
    21001 gcataccgcc gtgctcattg tcgatgacga tcgggatatt cgcgaatccc tcaccgacat
    21061 gctgcggcat gaaggttatc tcgtcgagag cgcggggtca gggaatgagg ccctgtctca
    21121 tgccaaacag cggcagtacg gcgcggccat tctcgacatc cagctgcctg acctcaacgg
    21181 actctcggtg ctgaaagtgc tgatggagtt ggacgcctcg ctccccgtga ttatcctgac
    21241 cgggaacgca acctccgaga ataccattgg ctcgctcgcc aagggcgcgt ttgcctacct
    21301 gactaagccg tacaacgcgc aggagctgaa agccgtcatt cgtcgagcgg tttcagtgaa
    21361 agggctcgcg gtacgcgccg aacatgtcga gcaagccctg catgccagcg aggagcgatt
    21421 ccgcgcgctg gtggaatcgg caaccgacgc catcgtcttg gctgaccact acggccatat
    21481 catttggtgg aatggggcgg ccgaacggat gttcggatat tccaaacgtg aggcgctggg
    21541 acagtccctc gccatgctca tgccggaacg cttccgttcg gcccatgaga caggcatcat
    21601 gcgcctgacc acagggtcgg ctgcgaaact gatcgggaaa accgtcgaac tggtaggact
    21661 gaccaaagcc ggggatgaat ttcccatcga actgtccttg gcctcctggc gtgccaagga
    21721 gggcgcgttc ttcagcggca tcatccgcaa cattacgcgg cgcaaaaagg cggaagaatc
    21781 cgtcgtccgg ctcagccacc agaacgcctt gattctcgac gccgcagggg aaggaatttt
    21841 cggccttgac cttgagggac gcacgacctt cgtgaacgcc acggcggcac gaatgctgga
    21901 gtggagtgcg gcagaattga tcggcaaacc ccttgcccca ctcctgtata gacttcatga
    21961 cgaaggagcc gagtatgtcc aggatccctt atgtctttcc gctgcagtac gcgacggagc
    22021 ggtgcaccgt gtacaaaacg atgtgttcac cagcaaaagc ggggtgtcat tcccggtcca
    22081 atacgtaacc agtccgatcc gtgaacagga tcgaccagtg ggcgtcgtgg tggtgtttca
    22141 agatatcacc gcgcggaagc agctggaatg tttgcaacag gcacaactct ccatcagcca
    22201 tatcctcgct cagaccagca cgctcgaaga agccgtcccc caactgctcc gcgtgatcgg
    22261 agaaatggga ccgtgggaga tgacgatctt ctggcacaaa gaaccgatga acgaaagact
    22321 ggtctgtgaa ggcacgtgga tgcgatcctc ctgccgctgg gatgaattcg ccacgatctg
    22381 ccggaatagc gaattccctc cggggatgga ttttccgggg gtcgtctgga gcacgaaact
    22441 gccactgtgg attcccgatg cgttgacaga cgcccgcttc acccgcgcgc cgaccgccgc
    22501 caggctcgga atacacgggg cctgcctcat ccccatccga accgggaccg tcatccatgg
    22561 cgtactggaa ctgtatgcag acaagcctcg cctgcctgat cgccaactca ttcacatctt
    22621 gaccgatacc ggcatgaaaa tcggacagtt tatcgaccgc gcgcaagccg ggtcggcatt
    22681 acgcgcgacc catcgaatga cgcagaattt gctggccagc ctgccaggcg ccatcttcct
    22741 ttgcgcccgt gacttcaaga tccaatatgc caataccctg gcgcatcact acttcgccgt
    22801 cgggggagcg tccttagtcg ggaggccgct gttcgaatgc ctcgcgctca gtgaggccgc
    22861 gacgcaacaa ttgatcaaag aatgggctgc gctcaatgcc gactcaccgc atcaggcccc
    22921 cgaacgcgaa tgcgaagtcg cttcacgact gtatcggtac cgcttcttcc cggtcgccac
    22981 tccggaaagc gctcagtatc aggtaggcat cgtgctgtgg gacgtcaccg acgacaaaca
    23041 tctgcaggat caactgattc aatcggagaa gcttaccagt ctgggcacaa tggtctcggg
    23101 gatggcgcac gagatcaata atccggctca agccatactc agcatggccg aactgattca
    23161 ggaagaaaaa gacccggcgg tgatcaagga atttgccgcc gatatcgtcg gctacgcacg
    23221 ccacgtctcc aatgtcgtgc gggatttcgc cggatacgct cgttcggctg gccgggacgg
    23281 cgaaacggaa atcgatgtgg cggagcgcct catggaagcc gtcaagatgg cccgccgagg
    23341 tccgcatttc ggctacgtcg atgtcgtcac gcgcttcgac gcccccactt ttctgcgcgc
    23401 ccgcaagagc gaactcgatc aggtcttcgt caacttgatc aacaatgcca cgcaagccat
    23461 ggacgggaaa ggccgcctga ccttaggcgc ggctcaagac ggtgcgtgga ttgatgtgac
    23521 gatttcggat accggcccgg gcattcccgc tgccgtgcgg cctcgcatct ttgatccatt
    23581 ttttactacg aaggcgccgg gaaaggggac ggggctgggt ctcagcatcg tgcataagat
    23641 cgtgactcgg catgcaggga cgattacggt ggacagcacc gaaggggaag gcaccacgtt
    23701 taggctgcgt tttccggcct tgttgcagta aaggggaaac aagcacgggt cgacgacgag
    23761 cccgcggagg gatttacgcg cgggaacgag aagagtaggc ccgcttaaag acggaccgca
    23821 atccgaagta ggcgaatgaa tccttcagat tggaatagag acgtttaaag gcgcccatgt
    23881 cgggattggt cacatactcg agcgtgagca caatccgctc ttcctgctcg ccgagcggcg
    23941 tcacggcatg ccagagcttg tctccattga agatcaccag atcgccgggc gctgtggcca
    24001 attccatatg taccggctgt ttacccggca catctttaaa cagatcacag actaagcggc
    24061 attgtttgga ctgatccagc aaccccagca gaatcgtgta ccgcgcgcct ttatagtaag
    24121 aggtatcgta gtggtatccg atgtggtcgc cggcttccgt gtagtaatac aaggcacagg
    24181 aatgcggatc gttgtcgggg cagagtagaa gattgaccct ggtcaactga ttgagcatgg
    24241 ctcgaaacgc gtctgaacga tacaggtcca tgaactgcgg ggcctgctcc ctcaccgtat
    24301 agtagctgac actgccgccc ttcttatgtc ccggaatgta attgcgattc aacccggatt
    24361 tgagcacctg cgcctgtgac gccagctcgg cctcgacaaa ctcgcgcggc agaaattggg
    24421 gaatatacag aaactcgttc tgctcccaat agtcgcgctc caaccggtcg agatccaggg
    24481 ccgaaatggc ccgctcaacc gcctctgtta cgctgcttgc acaggcctgc gtcatgcgtc
    24541 cctcactgag tccggattca tcatggttcc actgaggaca tcgtcttccg atgaacaaaa
    24601 cgccacttaa aaaaaagcaa gttgcttgca ggatggtcaa aaagtccgtc cagtgaggcc
    24661 gcagcgag
//
LOCUS       DAOQDS010000058        23684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_775_length_23684_cov_23.568793, whole genome shotgun sequence.
ACCESSION   DAOQDS010000058 DAOQDS010000000
VERSION     DAOQDS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23684
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_775_length_23684_cov_23.568793"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PK589_13865"
     CDS             complement(<1..620)
                     /locus_tag="PK589_13865"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNJ20742.1"
                     /translation="MTTTTADMSQPPQSPGVPPPAGSYRIHPKAIRARRNRRLLVVAL
                     LVLIATVGYLMYWYTHDRFWIRTDNAYVTGNLVPVAAQASGIITQVLAEETQFVNRGD
                     LLVRLDEHQAYAALGRARGRLGEEVRRIAALFINRKQLAEKLRSRMARLELAEHDMDR
                     YQRASPSGAISKQILQNTADKIASLEAEVRETQAELDSLDAQIGGTT"
     gene            complement(625..2133)
                     /locus_tag="PK589_13870"
     CDS             complement(625..2133)
                     /locus_tag="PK589_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNJ20743.1"
                     /translation="MRSGSTVGQRVWRCLGIGCVLLFGSCAWIPKGDPPAQYLDPPEM
                     TETLAEITSRLQNWPDDHWWEQFGNPELNGLIETALKDNPGLKHASARLRQATSLVKV
                     EGARLLPFLEADASLTYERISQHGVFAALNPEVAGIKIMYGIINPLSFRYEFDFWGKN
                     RAMLEGALGHAAAEEAETAEVRLRLTTGIARAYFRGQALQQQLTIVKTIVGIRRDLKN
                     LAETRFRLGLDNDQPFKIAVADYEAAFKRQAAIRDQLDVQRHLLARLAGKGPDEAAHL
                     FAKPMANIPKQIPAPDHLSIGLLVHRPDLAAALYRAHAASRLVRVATTQFYPTIDLTG
                     FVGFNALTLAKGTDKLANFLFSGQSFTYGLAPGLRMPWFEGGRLRGELGAQRAEYDAA
                     VELYNDTLLDAMREVADSLSAWHTTNEIMASHKRLVASLGEDWRLAKVRLVNGLDDDR
                     EVLRHRYPVLEQEYALRALESDQLVAAVDLIESLGGGYHNPDIEKRPKHNPS"
     gene            complement(2130..3728)
                     /locus_tag="PK589_13875"
     CDS             complement(2130..3728)
                     /locus_tag="PK589_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNJ20744.1"
                     /translation="MAAVYLRRLHGWRFVLFNAVLGLSHIVVLFNAGSYIALLPHVSG
                     DLGGVLPSFLTWAQTDFMVGLALGFPLARYLSGRIGDYRLLIGAFMVYSVASYLCGDS
                     RTLAQFVPARIVQGIAGGITLPIAQSMLLNEYPKRLKAVALTVWGLFSITPFTIGMPV
                     GGYIAYMLGWRFLFYLDFVLTLVIVGTLGALLYGRGFRRRYARFDLVGFLLLAVLLLG
                     LQTLLNMGNDFDWLDSPFLQVIAVVLLIAFPCFIIWELGERHPTLDLRLFLHRNFVIG
                     IISLTLGFFSIQGLLSLLIVQIQLILGYSSKLAGLALLPLVLLSAPIIAVMHYLCKYV
                     DARWLVCLNCLGFAWTFYWIGLYDDPHSYEELFWPMVVEGIFLGSFFTPITVLTLHGL
                     SGPLVMRAAEVANLLRVAAGAFGITFQGIVLFRRIHFHQLHLADHFGGRQSISYDPMG
                     QLAAKFQAAGLSVSEAQAKLGLVIKQEAAILGLNDAFLVASFIFVGLGGLVWFAHPTH
                     VPIASSPEDDLRVRRAEELIEEVP"
     gene            complement(3747..4619)
                     /locus_tag="PK589_13880"
     CDS             complement(3747..4619)
                     /locus_tag="PK589_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNJ20745.1"
                     /translation="MDYVKPYGVVDSMLAGGALKLSLPPRQLVLRGMLAGAYLGIGTS
                     MAVTVAVETGYWILGSLVFPFGLALAILLGAEIITGSFALLPVAAAHGQKNAGLSRVF
                     ANWGWVFLGNLLGSVLYATLFAIALTTGGDAPINAVGNKLIAIAQAKTTYYASHGSAG
                     LLAAFTKGMLCNWMVSLAVVAAYMSTSLSGKFVAIWGPTLLFFSQGFEHAVVNMFLMP
                     VGMMLGADVSLSTWWLWNQIPVTLGNLVGGMLFTGLAIYAAHHATAPAAAAAQAPAQP
                     VTARGEQAGYSPSY"
     gene            5037..6992
                     /locus_tag="PK589_13885"
     CDS             5037..6992
                     /locus_tag="PK589_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ20746.1"
                     /translation="MSDLLSNLPIARKLFLASVIPALTVLLLSILTYRSVTTFSDDEG
                     QLNDIYYSQRLASEYLRLVVDLETGFRGFVLTSQEHYLFPYRTAQDHVLNIGRTLEAQ
                     VSQQDDQRALITSVQQLVKQFISEKEVLIDAVKAEHPNEAREYIEEGKGRALMLKIRQ
                     DMGRFEHLTQTALNTKLAKIAQDRDTMLMTILGGGLFSLICMVAALHLIAHSITTPLA
                     RLATAVVTSDRNPIPQVPVMTRTDEIGNLARVMHAMSTAIHSHIEALQQSEGNLRQLN
                     QDLAGSEAKYRSIVDHAPFGIFTTKGMTLVFSNRYNSLLAGLNSTEEKDPDAVRRAIH
                     PEDRDRVIAEFAQAVVEGEPYETVFRFLHADGTIRKVLSRRIPIRDQSGQVVMYQGFN
                     VDITALDFMQTRLRRAERLATLGQVAAGIAHEIRNPLVGIGSTTSLLLDDTDASDPRR
                     PDLAVILQETKRLDRIVNQIIDYARPREIVAFTFDMAQLVHEVMKVLDEPLTRKQAVI
                     NLSAPAAPYMIQADRDQFKQVLLNILQNAIEATPQGETITVALAEQARGIESGLEITV
                     TDQGSGISPSHLPHVFEPFFTSGKPRGTGLGLAISRNILDAHGGDIALDSEEGRGTTV
                     RVWTPVRQQPQRMQEEGDHAGHDLRYG"
     gene            6964..8358
                     /locus_tag="PK589_13890"
     CDS             6964..8358
                     /locus_tag="PK589_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ20747.1"
                     /translation="MQATIYVTDDEPAIRSAIVKRLSRRHHTVTGFESGEDLVSAVAQ
                     QAPDLILLDLKMPGMGGIEALRKIRPLAPQTLIIMLTAYGTVENAVEAMRLGAYDFLI
                     KSVDLSGVDPVVDRALEFLALRHRVQFALEDQNSAYALANIDARSPAMQLLLGQVRDV
                     AENAKSTVLLQGETGSGKEFLARVIHCNGPRAAGPFVAVNCTAIPKELFESELFGYER
                     GAFTGAHQRKRGLLEKAEGGTLFLDEIGDLDPAMQAKLLRVLQERTFRRLGGTEDLSV
                     DFRLMTATNRDLKKDTARGTFREDLFFRLNVVTFELPPLRVRAEDILPLCMQAMLRFG
                     KEFGKEVIDIEPDAKELLQRYSYPGNIRELHNIIERAMILCHDKTLTAGCLPRELRDQ
                     ASTHVAVAMAQGEHPSLRIEMVLGQQTLADIESALIEEVVRLSDHNKTLAAKHLGLTR
                     FALDRRLKKQMEHE"
     gene            8435..9574
                     /locus_tag="PK589_13895"
     CDS             8435..9574
                     /locus_tag="PK589_13895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNJ20748.1"
                     /translation="MHDRNHTILPLIIGLSAALFAVDLFMPLGIAIGVLYAGIVTLAA
                     ASSHPRLPFLTAIASTPLIIAGAALGPQHAAIPVWVGVSNRIFSLMIVWISAVLLQQR
                     RRAEIELREAKDELEVRVDARTKELADVNRSLLQEISGHIQTEGFLRNSEQALATSRQ
                     ELRDLAARLLTVQEEERRRISRDLHDDINQRLAMLVVQAESLEANLPASAVGCSKELR
                     SIQDRLTELSDDVRHLAYQFHPSILDDLGLPVALQRLVDECAVRSNLAISFETDASPK
                     AIPQTVSTCLYRIAQECLSNIMKHAHASQAKVSLASTAEGITLTVQDDGVGFDTQQVV
                     DNPRGLGLVSMAERVRLVHGTVTIDSIPQQGTRLSIHVPRAEVSV"
     gene            9571..10215
                     /locus_tag="PK589_13900"
     CDS             9571..10215
                     /locus_tag="PK589_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ20749.1"
                     /translation="MTTPPRVLLADDHTLVLEGFRRIVEQRCEVVGAVEDGRALLEAA
                     MQLRPDLILLDISMPLLNGVDAGRQLKKLLPDVKLIFVTMHADPAYVSEAFKAGASAY
                     LLKRSAARELDQAIDAVLKGQYFVTALLTRDIVTGLSAGQSGLFAQRQELTPRQREVL
                     QLIAEGRTIKEIAALLNISPKTVEFHKAQIIFHLNLRTTAELTKYALAHGLTSA"
     gene            10400..10600
                     /locus_tag="PK589_13905"
     CDS             10400..10600
                     /locus_tag="PK589_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20750.1"
                     /translation="MSPTATRCQFCWRVEDNRTGGTDSAAWSDLPTFMQKHNLVSHDL
                     NFADGYCPQCTQLYGRLTQEHP"
     gene            11222..12598
                     /locus_tag="PK589_13910"
     CDS             11222..12598
                     /locus_tag="PK589_13910"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ20751.1"
                     /translation="MNRRSPMQWGNDQARFTRGSVNVWHLRLTYHMTIGCLVAMLSGM
                     AWSSPCLSWAASPSTMSSSASEPVYSMATVLDLALARNPTVASAEGTIEQNRGQQVAA
                     HTYLNPSVNANSGRGRMRDLGLFDPAVRESVTEFNLSVGQPIEWPSKRAARQRATEAG
                     VASASAGLAETRLNLIADVKVAFYDLLLAQRDLTLAQQNLATIEDVHRAVTTRVRLGE
                     SPQFEAIRSEVEVLKAKQSLTSANNRMRVGRVMLDTLTAGSLGPSYAIDGQLHRVGPG
                     FGIDILTERALTEHPTIMRLRKAVEQADHSLEFERQARVPNITIGGSYWREIGREAFT
                     GGVSFPTPVWDRRQGEIISAFGSKRKGEAEYLRARNELIRNISQHFQDAKTTADLIEV
                     YEKGLLRQAEEALRIAKFSFQQGASSLIEVLDAQRVQRQILLDYAQAQFDLSISLALL
                     ERAVGGNL"
     gene            12598..13827
                     /locus_tag="PK589_13915"
     CDS             12598..13827
                     /locus_tag="PK589_13915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ20752.1"
                     /translation="MAAACHDHHAEVSPRHARRWTARGISLLFVVLLASSGCDGPPPN
                     ATGANPPVAPAESGSIRLTAEKVKSAGITVQPLRRGEFRTFRDFPGTVEPDEHALAEI
                     TTLVRGRVIDVYADLGREVKGGTLLALLYSSELGMAQSAYLKATAKLNVADRAFRRAE
                     LLLKEKVIGLAESQRREGEMISLRAEQREARDRLLLLGLTDEDLRHLDKNQTIRSHVP
                     VVAPFDGRIIARNLTKGEVVETTEKLFVLADLSEVWVTAKIPEKDIPYIRTDQTGTGQ
                     LVEVHVSAYPGQIFQGSITYVGDVLDPATRTMRLRLELPNPQRKLKPEMYATVRVYSD
                     PEPNVLLIPETAIQRDRDRQFVFVEREPGLFEIRPVQLGDSNGKEVKVLGGVEDQEAV
                     VVTGSYVLKSELLGSQI"
     gene            13832..>14856
                     /locus_tag="PK589_13920"
     CDS             13832..>14856
                     /locus_tag="PK589_13920"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ20753.1"
                     /translation="MVSSLLQLALRQRVLVVVIAFALALGGLYAFQTIPIDAFPDVTT
                     VLVQVVTKAPGLSPEEIERFVTFPLELQLTGAPGLVEVRSLSKVGLSMIDVIFEDQVD
                     IYLARQVVLERVLEVQDQLPPGSISQLVPNTTGLGEVFQYVLKGPQDDAAGGLVTEQE
                     LMMRRTLQDWLIRPLLKGLPDVVDVNSLGGFVKQYQVMVDPGLLRKYNLGLHDVFAAV
                     QQNNANAGGNILEKNSERYIVRGVGLIKTVADIEQIVVKEVGGTPVYVRDVAEVRIGS
                     AIRHGAAVINGQQETVTGIVLMLRGGNAREVVQAVKTKIDDIHAHKLLPDGLRIVPFY
                     DRIELITA"
     assembly_gap    14857..14956
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14957..17106
                     /locus_tag="PK589_13925"
     CDS             <14957..17106
                     /locus_tag="PK589_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNJ20754.1"
                     /translation="PDGLRIVPFYDRIELITAALGTVYKALLEGMLLVVVVLFLFLGN
                     VRSALIVTATLLLTPLATFILMDRVGLTANLMSLGGLVIAIGMMVDGSVVVVENIYRH
                     LSEHRDSREGNVSTILRAAMEVGQPVVFGILIIIIVFFPILSLQGMEGKMFQPLAYTI
                     IIALLISLLLSLTLSPVLCSLVLKDAREEDTLLLRWIKRAYMPALGWALRHRTVVLGS
                     ALVMLAASLALFPFLGGEFIPILNEGSVSPQTIRLPGISLEKSIEIEKEMQRAILEFP
                     EARMVVSKIGRTELGNDPQEPNSSDPVVSLVPMDQWKTATTKAQLDDAIRRRLQRIPG
                     ANFLISQPIQQRVDELVSGVRSEATVKVLGDDLDVLRATADKIQTIMTGIDGVKDVRV
                     EQLFGQPYLTIDIDRGQIARHGINVAHVREIITTAIGAEPATRVYEGQRRFDLILRYP
                     EQYRDSVDTVSNILLTTPTGALIPLGNIAKVELREGPALISREGLQRRIYVGFNTFGR
                     DIESIVHEAQTKIARQVPLPTGYRLQWGGSFENMQRAMARLKIVVPLTIGVIFLILFS
                     SFNSLRHAALIILNLPFALIGGIAALWLTGEYLSVPASVGFINLFGVAVLNGIVLVSY
                     INKLRAEGMAGEEAVVTGCSLRLRPVLMTAVVALLGLLPLALSHGIGSEVQRPLAIVV
                     IGGLVSSTLLTLIVLPVLYQWFDRPDKSAAPAN"
     gene            17238..17552
                     /gene="sugE"
                     /locus_tag="PK589_13930"
     CDS             17238..17552
                     /gene="sugE"
                     /locus_tag="PK589_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HNJ20755.1"
                     /translation="MAWTFLIVAGLFEICWAIGLKYTDGFSKFWPTAGTLMAMAASFG
                     FLAQALKSIPVGTGYAVWTGIGAAGTAVLGIVLFAESVSIVKVFSLLCIVLGIIGLKG
                     SA"
     gene            17594..18781
                     /locus_tag="PK589_13935"
     CDS             17594..18781
                     /locus_tag="PK589_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:proton antiporter"
                     /protein_id="HNJ20756.1"
                     /translation="MLPVATPEPQESTLLRFPNRNTPPRYDRAIGEWALVIPLGTAGL
                     FFVHGHEWLHDLSNPLRLSAMLAWLLLVIAFSAFAVLRHAEHLAARLGEPMGTVILTL
                     SVTGIEVMMIAAFMYTGNGNASLARDAMFAVVMIVLNGMVGLSLLLGGLRYHEQTYNL
                     QGANAFLAVIVPLAVLGLVMPSYTISSPGPTFSPHQAIFLIVMSLGLYGVFLAIQNLR
                     HRNYFVAPRRQSRRNAAILHEHQPSPGGSVWFHTLLLLAYLLPLVILSEHVATPIDYT
                     LRLWNAPHALGGVLVAGLVLAPESLGAVRAALANQLQRSVNILLGSVLASISLTIPAV
                     LAIGFFTDSTILLGIDPVDTILLVLTFVVSMLTFAVRRTNVLLGAVHLLLFLAYLMLI
                     FEK"
     gene            18963..20384
                     /locus_tag="PK589_13940"
     CDS             18963..20384
                     /locus_tag="PK589_13940"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ20757.1"
                     /translation="MSGAKSKTFTILLVEDNAGDVDLFLRTVEHELPRHEDDEVTLVL
                     NATAEGALERLRQQPIDLVIADVRLPGMSGIELLRQVQDLNRRIPVIILSRVDATETV
                     IDAMRHGAFDYIVEPYDCMDLAARIHRAMRMSEILLQGSPDEPAAPRIPFKNLVGVSP
                     AIRNVMAMIEAIARVPSTTLIIGETGTGKELIAKAIHERSLDSDGPFQVVDCTTFSEG
                     TVESELFGHVRGAFTGAVADRTGLIESGSHGTVFLDEIGDLPATLQAKLLRVLEAGEV
                     RAVGSTQQRKVNVRFVAATNQDLAEKVKRNEFRKDLFFRLNVMVIRVPPLRERTEDIP
                     VLARHFIARYAKEFTKRVDDLHPAAVTELVAYPWPGNVRELRNVMERAVMLAKGDRIG
                     IADVAAMLAVPDERQRDTPDEDYLHLPYAKAKEQVLEAFNRRYISTKLTIHQGNVTHA
                     AQDAGLPRSYFHEIMRRYSKDEK"
     gene            20870..>23684
                     /locus_tag="PK589_13945"
     CDS             20870..>23684
                     /locus_tag="PK589_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNJ20758.1"
                     /translation="MQVSVSRRQFLKISAGTVAAVAVADKVLALTALQPVIEVGNPLG
                     EYPDRSWERVYHDQYRYDSSFTWCCSPNDTHGCRVRAFVRNGVVMRVEQNYDHQTYED
                     LYGNRGTFAHNPRMCLKGFTFHRRVYGPYRLKGPLMRKGWKQWMDDGSPELTPDVKRK
                     YKFDSRFLDDLNRVSWDSAFTYVAKGAILIATRYSGEAGARRLREQGYAPEMIEMMKG
                     AGVRCFKHRAGMPVLGIVGKMMNTRFNGGCLPLLDSWIRKVDAEKAQGGKYYSNYTWH
                     GDQDPSHPFWNGTQNCDVDLSDMRFSKLNTSWGKNFVENKMPEAHWKLESIERGARIV
                     VITPEYNPTAYRADYWIPLRPETDGANFLGAAKIIFDENLQDIDYIKEFTDLPLLVRT
                     DTLQYLDPRDVIADYKFPDFSKSYSGRIQALKPEQIERLGGMMVWDLGKQQAVPLHRE
                     QVGFHFKASGIDPALTGTHRVKLLNGREIDVMPLYQMYQVHLQDYDLDTTHQITRAPK
                     DLIVRWARDSGTVKPAAMHNGEGVCHYFHMTEMGRAAAFIMTITGNIGKFGTGCHTWS
                     GNYKAGIWNAVPWSGAGLAVHTGEDPFNLTLDPNAHGKEIKTRSYYYGEEVGYWNHGD
                     TALIVNTPKYGRKVFTGKTHMPSPSKVRWVTNVNILNNAKHHYDMVKNVDPNIEMIVT
                     QDIEMTSDVNHADVAFACNSWMEFTYPEMTGTVSNPWIQIWKGGIRPLYDTRNDADTF
                     AGVAAKLSEMTGDARFRGVFHFVYMNRVDVYPQRMLDASATCYGYSADVMLKSEKGWM
                     VMGRTYPRHPLWEETNESKPQWTRSGRIETYRIEPEAIEYGENFISHREGPECTPYLP
                     NAIFSNNPFIRPDDYGIPITAQHHDDKHVRNIKLPWAEIKRHPNPLWEKGYQFYCVTP
                     KTRHRVHSQWSVNDWVQIYESNFG"
BASE COUNT         5049 a         7132 c         6744 g         4659 t
ORIGIN      
        1 gtcgtgccgc cgatctgcgc atcgagcgag tcgagttccg cctgtgtttc ccgtacctcc
       61 gcttccaggc tcgcaatctt gtccgccgta ttctgcaaga tctgcttgga gatggcgccg
      121 ctcggggatg cccgttgata gcggtccatg tcgtgttcgg ccagttctaa acgagccatg
      181 cgcgaccgta atttctccgc cagttgtttg cgattgatga atagcgccgc gatccggcgg
      241 acctcttccc ccaatcgtcc acgcgccctt cccaaagcgg cataggcttg atgttcatcc
      301 agtcgcacca gcaagtcacc gcggttcacg aactgtgttt cttccgccaa gacctgcgtg
      361 atgataccgg acgcctgtgc cgccaccggc accaggttac cggtaacata ggcgttgtcg
      421 gtccggatcc aaaaccgatc gtgggtgtac caatacatga ggtagccgac ggtggcgatc
      481 aggaccagca gagcgacgac caataggcgc cggttccggc gggctcgtat ggccttcgga
      541 tgaatgcgat aggagcctgc cggtggcggt actcctgggg attgcggagg ttgagacata
      601 tctgccgttg tggtggtcat acatttaact cgggttgtgt ttcggtcgct tttcgatgtc
      661 cggattgtga tacccgccgc ccagggattc gatcaggtcg acggcggcca ctaattgatc
      721 gctttccaac gctcgcaggg catattcctg ttcgagcacc ggataccggt ggcgcagcac
      781 ttcgcgatca tcgtccaggc cgttgacgag gcggactttt gccaaccgcc agtcttcgcc
      841 cagcgacgcc accagccgtt tgtgcgacgc catgatctca ttggtggtgt gccaggcact
      901 gaggctgtcg gctacttccc gcatcgcatc gagcagcgtg tcgttataga gttccactgc
      961 cgcgtcatat tccgcgcgct gtgcgcccaa ctcgccccgc aaccggccgc cttcaaacca
     1021 cggcatccgc aatccggggg ccaggccata ggtgaagctc tgaccgctaa agagaaaatt
     1081 cgccagcttg tcggttccct tggccaacgt cagggcattg aatcccacaa agcccgtcag
     1141 atcgatggtg ggatagaact gcgtcgtcgc caccctgacc aaccgtgacg cggcatgcgc
     1201 gcggtacaag gctgccgcca gatcagggcg atggaccagc aggccgatcg acagatgatc
     1261 cggcgcagga atctgtttgg gaatgttggc cataggcttg gcaaagagat gggctgcctc
     1321 gtccggcccc ttcccggcta ggcgcgcgag caaatgccgc tgcacatcca attgatctcg
     1381 gatggccgct tgccgcttga atgccgcctc gtagtcggcg acggcgatct tgaacggctg
     1441 atcgttgtcg aggcccagac gaaaccgggt ctctgcaaga tttttgagat cgcggcggat
     1501 gccgacaatc gtcttcacga tcgtcagttg ctgttgcagg gcctgccccc ggaaataggc
     1561 ccgggcaata ccggtggtca atcgcagacg cacttcggcg gtttccgcct cttcggcagc
     1621 cgcgtggccc agcgctccct cgagcatcgc ccggttcttc ccccagaagt cgaattcata
     1681 ccggaagctg agcgggttga tgatcccgta catgatcttg atgcccgcca cttcgggatt
     1741 cagcgcggcg aacacgccat gttgggagat acgctcatag gtcaaggagg catcagcttc
     1801 caggaacggc aacaaccggg cgccttccac ctttacaagc gaggtggcct gccgcagtcg
     1861 cgcggacgca tgtttcagcc cgggattgtc cttgagcgcg gtttcgatca gcccgttcaa
     1921 ctcagggttc ccgaactgtt cccaccaatg atcgtccggc cagttctgca gccggctcgt
     1981 gatttcagcg agtgtctccg tcatctccgg cgggtcgaga tattgcgcag gaggatcgcc
     2041 cttgggaatc cacgcgcagc tcccgaagag caggacgcag ccgatcccca ggcacctcca
     2101 cactcgctgt cccacggttg aacccgatct catggcacct cctctatcaa ctcctcagcg
     2161 cgtctcacgc gcagatcgtc ctcaggactg gaggcgatgg gcacatgcgt cggatgggcg
     2221 aaccagacca gcccccccaa cccgacaaag atgaaactgg ccacgagaaa cgcatcgttg
     2281 aggccaagta ttgccgcttc ctgcttgatc accagcccca acttggcctg ggcttcggaa
     2341 acagacaacc ctgccgcctg gaatttcgcg gccaactgtc ccatcggatc gtatgaaatc
     2401 gattgccggc ctccgaaatg atcggccaag tgcagctgat ggaagtgaat gcgccggaac
     2461 aggacgatgc cttgaaacgt gatcccaaat gctccggcgg caacccgcaa caaattggca
     2521 acttctgcgg cgcgcatcac caacggaccc gagaggccgt gcaaggtcag cacggtgatc
     2581 ggcgtgaaga aggaacccag aaaaattccc tccacgacca tgggccagaa caattcttcg
     2641 taggaatgcg gatcgtcgta cagtccgatc caataaaacg tccatgcaaa accgagacag
     2701 ttgaggcaga ccagccagcg tgcatcgaca tacttgcaga gataatgcat gacggcgatg
     2761 atcggggcgc tcagcaacac caacggcaac aacgccagac cggccagctt cgaggaatac
     2821 ccgagaataa gttggatctg gacgatcaga agagataaca gcccttggat cgagaagaac
     2881 ccgagtgtca ggctgataat cccgatgacg aaattgcgat gcaggaacag ccggagatcc
     2941 agcgtcggat gccgttcccc caattcccag ataatgaaac agggaaaggc gattagcagc
     3001 accaccgcaa tcacttgaag gaagggcgag tccaaccagt cgaaatcgtt gcccatgttg
     3061 agcaacgttt gcagccccaa caacaggacg gccagcagca gaaacccgac caggtcgaag
     3121 cgagcatacc gtcgacggaa gccgcggccg tacaacagag cacccaacgt gccgacaatc
     3181 accagcgtca gcacgaaatc cagataaaac aggaagcgcc acccgagcat gtaggcgatg
     3241 tagccgccca ccggcatgcc gatggtaaag ggtgtgatgc tgaacagtcc ccagacggtc
     3301 agagccacag ccttgagccg tttggggtat tcgttgagaa gcatggattg ggcaatcggc
     3361 agggtgatgc caccggcgat gccttggacg atacgggcag gaacgaattg agcgagcgtt
     3421 cgactatcgc cgcacagata ggaggccacg ctatacacca tgaacgcgcc gatcaggaga
     3481 cggtagtcgc cgatccgtcc cgacaggtac cgcgcgagcg gaaagcccaa tgccagcccg
     3541 accatgaaat cggtttgcgc ccaggtcaga aaactcggca gcaccccgcc gagatcccct
     3601 gagacatgcg gcaacagcgc gatgtaggag ccggcgttga acaagacgac gatgtgcgac
     3661 aagccgagga cggcattgaa gagcacgaat cgccatccat gtaatcgcct gagatacacc
     3721 gcagccatga cgacgttccc tcactgctaa tacgacgggg aatatcccgc ctgctcgccg
     3781 cgggcagtca caggctgagc cggcgcctga gctgcggcgg cagccggcgc ggtcgcgtga
     3841 tgcgccgcgt aaatcgccaa tcccgtaaac agcatgccgc cgaccagatt acccagcgtg
     3901 acgggaatct gattccagag ccaccaggtg gagaggctca catccgcgcc gagcatcatg
     3961 cccaccggca tcaaaaacat gttgaccacg gcatgttcaa aaccctgaga gaagaacaag
     4021 agcgtcggac cccagatcgc gacaaacttg ccggacaacg atgtcgacat ataggctgca
     4081 accacggcga gactgaccat ccagttgcac aacatgccct tggtaaatgc cgccagaaga
     4141 cccgcggaac cgtgcgatgc gtagtaagtc gttttcgcct gcgcgatggc gatcaattta
     4201 ttgccgaccg cattgatggg cgcatcgccc cccgttgtca gcgcaatcgc gaacaacgtc
     4261 gcatagagca cgctgccgag gagattaccg aggaacaccc atccccaatt ggcaaagaca
     4321 cgggagagcc cggcgttctt ttgaccgtgt gccgccgcga caggcaataa cgcgaagctg
     4381 ccggtgatga tctccgcgcc gagcagaatg gccaaggcca atccgaacgg aaagaccaga
     4441 ctgccgagga tccaataacc ggtttcgacg gccaccgtca cggccatgct cgtcccgatc
     4501 ccgagatagg caccggccaa catgccacgc agcacaagtt gccgcggggg taaactcagc
     4561 ttcagtgcgc caccggccaa catgctgtcg acgacgccgt acggcttcac atagtccatt
     4621 gtctcctcct tgttcggtct gccattccgc gcactccgcg attcaccgac ccggtcaccg
     4681 agacttgacg atagtcgacc tgagcaaggc gcatgcctca tctagaacag cccgttcagg
     4741 ccgtcgccac gacaacgaat ggtgacgtgc cgcatctgcc agcgcagccg ttcgcacaat
     4801 cgcatgacgc gtgaaaccag cgtggagacg ggcgggatcg cacgggcgca cggtgcgaaa
     4861 tcgctcattc ctctgcgcgg cggcagcttt gaacgctgga ggaaggagtg aaacaacaac
     4921 cgcaggcgat aagaagggtc accggcgtga gcgcccactc aggacgacaa tcaggctgca
     4981 aagttgcaca gtggtgggag tgcagactac gatagcgacg gagactgaga tctgccttga
     5041 gtgatctgtt aagcaatcta cccatcgccc gcaagttatt ccttgcgtcg gtgattcctg
     5101 cgctcaccgt cctgctgctc agcatcttga cctaccgtag cgtcacgact ttttccgacg
     5161 acgaaggtca attaaacgac atctactatt cccaacggct ggcctcggaa taccttcgcc
     5221 tggtggtcga tctggaaacg ggattccggg gctttgtgct gacgagccag gagcattacc
     5281 tgttccccta tcgcaccgcg caagaccacg tcctgaacat cggccgcacg ttggaagctc
     5341 aggtgtccca acaggacgat caacgggcgc tcattacttc tgtgcagcaa ttggtcaaac
     5401 agttcatcag tgagaaggaa gtgctcatcg atgccgtgaa ggccgagcat cccaacgaag
     5461 cgcgggagta tatcgaggag ggcaaaggcc gggcgctgat gttgaagatc cgccaggaca
     5521 tgggccgatt cgaacatctc acgcagactg cgctgaatac caagctggcg aagatcgcgc
     5581 aggaccgcga cacgatgctg atgaccatcc tgggcggcgg actcttttcc ttgatctgca
     5641 tggtggcggc gttgcacctg atcgcccatt ccatcaccac cccgctggcc cgcctggcca
     5701 cggcagtggt cacctcagac cgcaacccga tcccgcaggt tcccgtcatg acgaggacgg
     5761 atgaaatcgg caatctggcg cgggtcatgc acgccatgag tacggccatc cattctcaca
     5821 ttgaggccct gcaacaatcg gagggcaact tgcgccagct caatcaggac ctggccgggt
     5881 cggaagccaa gtatcggagc atcgtcgacc atgcgccgtt cggcatcttc accaccaaag
     5941 ggatgacgct ggtcttcagc aatcgctaca atagtttgct tgccggactg aattcgacgg
     6001 aagaaaaaga tcccgatgcg gtgcggcgtg ccattcaccc ggaggatcgg gaccgggtca
     6061 ttgcggaatt cgcccaggcg gtggtggaag gagaacccta cgaaaccgtg ttccggtttc
     6121 tccatgcaga cggtacgatc cgcaaagttc tgagccggcg tatcccgatt cgtgatcagt
     6181 ccggacaggt cgtcatgtat cagggattca acgtggacat taccgcgctc gacttcatgc
     6241 aaacccgcct gcgccgggcc gagcgtctag cgactctcgg acaggtggcg gcgggcattg
     6301 cacatgagat ccggaacccc cttgtcggca tcggctcaac cacctcgctg ctgctcgacg
     6361 acaccgatgc gtctgatcct cggcggccgg acctggcggt cattctccag gaaacgaaac
     6421 ggctggatcg gatcgtcaac cagatcattg actatgcccg ccccagggaa atcgtggcgt
     6481 ttaccttcga catggcacag ctggtgcatg aagtgatgaa ggtcctggac gaacctctga
     6541 cgcggaaaca ggcagtcatt aacctttctg cccctgccgc tccctatatg atccaggccg
     6601 accgcgatca gtttaaacag gtgttgctca atatcctgca gaacgccatc gaagccacgc
     6661 cacaaggcga gaccattacc gtcgccctgg cggaacaggc acgcggaata gaatcggggc
     6721 tggaaattac cgtgacggac cagggcagcg gcatcagtcc cagtcatctg ccacacgtct
     6781 tcgaaccgtt ctttaccagt gggaagccgc gcggaacggg cttgggattg gcgatctccc
     6841 gcaatattct cgacgcgcac ggaggagaca tcgccctgga cagtgaagag ggacgcggca
     6901 ccaccgttcg cgtgtggacg cccgtgcgcc aacagccgca acgaatgcag gaagaagggg
     6961 accatgcagg ccacgatcta cgttacggat gacgaaccgg cgattcgctc cgctatcgtc
     7021 aaacgccttt cacggcgcca ccatacggtt accggctttg aatccggaga agacctggtc
     7081 agtgccgtcg cgcagcaggc ccccgacctc attctcctgg atctcaaaat gcccggcatg
     7141 ggcggcatcg aggcactgcg aaaaatccgc ccccttgccc cgcagacgct catcatcatg
     7201 ctgaccgcct atgggacggt cgaaaacgcc gtcgaagcca tgcgcttggg cgcctacgac
     7261 tttctgatca agtccgtcga tctctccggg gtcgatcccg tcgtcgatcg cgccttggaa
     7321 tttctcgcgc tgcggcaccg cgttcaattt gcactcgagg atcagaacag cgcctacgcc
     7381 ctggccaata tcgatgcccg cagtccggcg atgcaactgc tgctcggcca ggttcgagat
     7441 gtggccgaaa acgccaaatc aaccgtatta ctccaaggag aaacgggcag cgggaaagaa
     7501 tttctggcgc gcgtcattca ctgtaacggg ccacgtgccg ccggtccgtt cgtggccgtc
     7561 aactgcaccg ccattcccaa agagctgttc gaaagcgagc tgttcggcta tgaacgaggc
     7621 gcctttaccg gcgcacacca acggaagcga ggactcctgg agaaggcaga agggggaacg
     7681 ctctttctgg acgagatcgg cgacttagac ccggccatgc aggccaaatt actgcgcgtg
     7741 ctgcaggagc ggaccttccg gcgcctgggc ggcacggagg acctgtcggt cgattttcgg
     7801 ttgatgaccg cgaccaatcg tgatctcaag aaagacacgg ctcgcggcac cttccgcgaa
     7861 gacctcttct tccggctgaa cgtcgtgacc ttcgaacttc ctccgttgcg cgtacgcgcg
     7921 gaagacattc ttcccctttg catgcaggcc atgttgcggt tcgggaaaga gttcggcaaa
     7981 gaagtcatcg acatcgaacc cgatgccaag gaattgctgc agcgctatag ctatcccggc
     8041 aacattcgcg aattgcacaa tatcatcgaa cgagccatga ttctgtgtca cgataagacc
     8101 ttgaccgccg ggtgccttcc gcgtgagctt cgcgatcagg cttcgacgca tgtggcggta
     8161 gccatggcac aaggcgaaca tccctcgctc cgcattgaga tggtgctggg acaacagaca
     8221 ctggcggata tcgaatcggc attgattgaa gaagtggtgc gcctgtcgga ccataacaaa
     8281 accttggcag ccaagcattt ggggttgacc aggtttgccc tggatcggcg gctaaaaaaa
     8341 cagatggagc acgagtagat cgccggcaca ccgcgcggtc tgccgttgca gcaatcagcc
     8401 gggaagcaga taatgaggac acgacgggtc tgcgatgcat gatcgcaatc acaccattct
     8461 tcccctcatc atcggcttga gtgccgcgct gttcgcggta gatctgttca tgccgctcgg
     8521 catagcaatc ggagtgctct atgccggaat cgtgactctg gcggccgcct cctctcaccc
     8581 acggttaccg ttcctcaccg cgattgcctc cacgccattg attatcgccg gtgcggcgct
     8641 gggcccgcag catgccgcca tccctgtgtg ggtcggtgtc agcaatcgca tcttcagtct
     8701 gatgattgtc tggatttccg ccgtgctgct gcaacagcgg cgaagggccg agattgaact
     8761 acgcgaggcc aaggatgaat tggaggtgcg agtagatgcc cgtaccaagg aactggctga
     8821 tgtcaatcgc agcctcctgc aggaaatttc cgggcacatc caaacagaag gatttctccg
     8881 taacagcgaa caggccctcg cgaccagtcg ccaggagctg cgcgaccttg ccgcccgcct
     8941 gctgaccgtc caggaagaag aacgccgacg catctcgaga gacctgcacg atgacatcaa
     9001 ccagcgcctg gccatgctcg tcgtccaggc cgaatcgtta gaagccaatc ttccggcgtc
     9061 cgctgtcggc tgcagcaaag aacttcgctc gatccaggac cggttgaccg agctctccga
     9121 cgatgttcgt cacttggcct atcaatttca tccgtccata ttggacgacc tcggacttcc
     9181 ggtggcattg cagcgcctgg ttgacgagtg cgcggtccgc tcgaacctgg ccatttcatt
     9241 tgaaaccgat gcgtctccga aagccattcc tcagacggtg tcgacctgtc tctatcgcat
     9301 cgctcaggaa tgcctgtcca atattatgaa acatgcccat gcctcgcagg caaaggtctc
     9361 gttggcctcg acagctgaag ggatcaccct caccgttcag gatgacggcg tcggattcga
     9421 tacacagcag gtggtcgaca atccgcgcgg cctcggcctg gtgagcatgg ccgaacgggt
     9481 gcgcctcgtt catggcaccg tgaccatcga ttcaatccct caacaaggca ctcgcctctc
     9541 aattcacgtt ccgcgcgcgg aggtttccgt atgacgaccc cacctcgagt actcctggcc
     9601 gacgaccata ccctggtgtt ggaaggattt cggcgaatcg ttgaacagcg ctgcgaagtc
     9661 gtaggcgcgg tggaagacgg gcgggccctc ctcgaagccg ccatgcagct gcgtcccgat
     9721 ctcattctgc tcgacatttc catgccactg ctcaacggag tcgacgcggg acgacagttg
     9781 aagaaactcc tcccggacgt aaaactgatc ttcgtcacga tgcatgccga tccggcgtac
     9841 gtcagtgaag ccttcaaagc cggcgcctcc gcctatctct tgaaacgttc cgccgcccga
     9901 gagttggacc aggccatcga tgcagtcttg aagggacagt attttgtgac tgcgctgctg
     9961 acgcgggata ttgtgaccgg gctctccgct gggcagagcg gcctctttgc gcaacggcag
    10021 gaattgacgc cgcgccagcg tgaagtgctg cagctcattg ccgaaggacg caccattaaa
    10081 gaaatcgcgg cgctcttgaa catctctccc aaaaccgtcg agtttcataa agcacagatt
    10141 attttccatc tcaatctccg caccaccgcg gagttgacga aatatgccct ggcgcatgga
    10201 ttgacctccg cctagcgcga ccttttgcaa ccgccacacg acagcccttc gaacccgtcg
    10261 catcgacgcg tctgacctcc acctaggaca tcacctagtt gtgaccgcgc accacattcg
    10321 ctatggtggt ggcgatcagc gaggcctgat cagaggcgct tccgccacac tccggacact
    10381 actgcaggag agcgggaaca tgagcccaac agccacacga tgtcagtttt gctggcgggt
    10441 tgaagacaat cgaaccggcg ggaccgattc agccgcctgg tctgacctgc cgaccttcat
    10501 gcagaaacat aatctggttt ctcacgatct gaattttgca gacggatatt gcccgcagtg
    10561 cacgcaactc tacggtcgtc tcacacaaga acatccgtag acgtggccat gcgggccgga
    10621 cgaggaataa ggatcattcg cggaatgaac aacaatcgag aacatccttc gaggtggcgc
    10681 cggtgtcact cgagtgagtg caccacaagc ggatctgagc gtaatacagg ttcggacacc
    10741 gatgcagata ccaacagtga ccaacctcga cccttctcga atcgcatcgt cagccggtat
    10801 aggagacggg cctctcgcgc ccgcccgcaa ccggaggccc gctatcagtg gcacggagcg
    10861 cgtcatccga accgacgcac cgggtagtgt cttcgccagg tcagctccgt aagtcacaca
    10921 gtactggact cgctagaaca cgagtcatcg ggagtcccct cctacaccac acagtctgtt
    10981 tgctcgctgg aaccactgac ctggcctcca ttactcgctg atccttgaca tcgcgggtcg
    11041 ctcgttagac ttcactccgt caagctgata cggtacttga aagagaccgt gtgcatttga
    11101 tgcagcgccg ccacaggcgg tggcgcaaga cggtgcggag ggagacatgt tgacgaggaa
    11161 cgatgcgaga cgacgcagag ggcgccaagc gtggcagaaa caaggagatt gtgatttcgc
    11221 aatgaatagg cgatcaccga tgcagtgggg aaacgatcag gctcggttca caagaggaag
    11281 cgtcaatgtc tggcacctgc gtctcacgta tcacatgacg atcggctgcc tggtggcgat
    11341 gctgtcggga atggcctggt cgagcccgtg tctgagttgg gccgcctccc cttcgaccat
    11401 gtcctcttca gcctctgaac cggtctattc catggccact gtgttggatc tggccttggc
    11461 acggaacccg acagtggcga gcgcggaagg gaccatcgaa caaaaccgcg gccaacaggt
    11521 cgccgcccac acttacctca atccttcggt caacgcgaat agcggacgcg gcagaatgcg
    11581 agatctgggg ctgttcgatc ccgccgtgcg tgaatcggtc accgaattca atctgagcgt
    11641 ggggcaaccg atcgaatggc cgtccaaacg cgccgcgcgg cagcgtgcca cagaagccgg
    11701 cgtcgcgagt gcgtcagccg gattggcgga aacccggctg aatctgatcg ccgacgtcaa
    11761 agtcgcgttc tacgacctcc tcctggcgca acgcgacctc accctcgcgc agcaaaattt
    11821 ggcaacgatt gaagacgtac atcgggccgt caccacgcgg gtgcgtttgg gagaaagccc
    11881 gcagttcgaa gcgattcgtt ctgaagtgga agtcctcaag gccaagcagt cgctcaccag
    11941 cgccaacaac cgcatgcgcg tgggtcgcgt catgctggat accctgacgg ccgggtccct
    12001 tggccccagc tatgccattg acggccagct gcaccgtgtc ggcccgggat tcggcatcga
    12061 catcctcacc gaacgcgcgc tgacagaaca tcccacgatc atgcgattgc gaaaggccgt
    12121 cgagcaggcc gaccatagtc tggaattcga acggcaggcc cgtgtgccga acattacgat
    12181 cgggggcagc tactggcggg aaatcgggcg agaagcgttt accggcggcg tgtcattccc
    12241 gacacccgtg tgggaccggc ggcaaggtga aatcatctcc gccttcggaa gcaagcggaa
    12301 gggtgaggcc gaatacctgc gggcccgtaa tgaactcatc cggaatatca gccaacattt
    12361 ccaagatgca aaaacgacgg cggacctcat cgaggtctat gaaaaggggc ttctccgcca
    12421 ggctgaagaa gctctccgga tcgccaagtt cagttttcaa cagggcgcat ccagcctgat
    12481 cgaagttctg gacgcccaac gcgtccagcg ccagattttg ctggactatg ctcaggctca
    12541 attcgatctt tccatatcgc tcgcgctatt ggagcgcgct gtgggaggga atttgtaatg
    12601 gcagccgctt gtcacgatca ccacgctgag gtttcgccga ggcacgcccg gcggtggaca
    12661 gcgcgcggca tttcgctgct cttcgttgta ctgctggcct cgtcaggatg cgacggcccg
    12721 ccgccgaacg ccacgggcgc aaacccgcct gtcgcccctg cggaaagcgg ttcgattcgt
    12781 ttgacggccg aaaaagtcaa atcggcaggg atcaccgtgc aaccgctccg gcgcggcgaa
    12841 ttccggacct tccgggattt ccccggcacg gtggaaccag acgaacacgc gctcgccgaa
    12901 atcaccaccc tcgttcgcgg gcgcgtcatc gacgtgtatg cagatttggg ccgggaggtg
    12961 aagggcggca cgctgctggc cctcctctat agcagcgagc tgggcatggc acagtctgcc
    13021 tatctcaagg caacggctaa gctgaacgtc gccgatcggg cgtttcgccg ggctgaactg
    13081 ctcctgaagg aaaaggtcat cgggctggcc gaatctcaac ggcgtgaagg agaaatgatc
    13141 agtctccgcg ccgaacaacg ggaagcccgg gatcgcttgc tcttgttggg attgaccgat
    13201 gaagacttgc ggcacttgga caagaaccag acgatccgat cccacgttcc agtggtggcc
    13261 ccattcgacg gccgtattat tgcgaggaat ctgaccaaag gcgaagtcgt cgaaactacg
    13321 gaaaaactgt tcgtccttgc cgacctctcc gaggtctggg tgacggcgaa aattcctgaa
    13381 aaggacattc cgtatatccg caccgaccag acgggaacgg gccaattggt cgaggtgcat
    13441 gtctccgcct accctggaca aatcttccag ggcagtatca cctatgtggg ggacgtgctc
    13501 gaccctgcga cgcgcaccat gcggctccga cttgagctac caaaccccca acgaaaactc
    13561 aagcccgaga tgtacgcaac ggtgcgggtg tactcggatc cggagcccaa cgtgctgctc
    13621 attccggaaa ccgccatcca acgcgaccgg gaccgccaat ttgtcttcgt tgagcgtgag
    13681 ccggggctgt tcgaaatacg accggtgcag ctcggcgatt cgaatgggaa agaggtcaag
    13741 gtgctcggcg gcgtcgagga ccaggaggcg gtggtcgtca caggatccta cgtgctcaaa
    13801 tccgagttgt tgggatcgca gatctgacga catggtatcc tctctgctgc agctggccct
    13861 ccgccaacgt gttctggtcg tcgtcatcgc cttcgcactc gccctcggcg ggctctatgc
    13921 cttccagacg attccgatcg atgccttccc cgacgtcacc accgtgctgg tccaggtcgt
    13981 gaccaaagcg ccgggactat ccccggaaga gatcgaacgg ttcgtcacct tcccccttga
    14041 actccagctg accggagccc ctggcctcgt cgaggtgcgc tcgctctcca aggtcggcct
    14101 ctcgatgatc gatgtcatct tcgaggacca ggtcgatatt tacctggcac gccaggtcgt
    14161 gctggaacgg gtgctggagg tccaggacca gctgccgccc ggctccatct ctcaattagt
    14221 gcccaatacc accggcctcg gcgaggtctt ccagtatgtc ctgaaaggcc cgcaggatga
    14281 tgcggcaggc ggcctcgtca cggagcagga actcatgatg cgccggacac tgcaggactg
    14341 gctgatccgg cctctcctga aagggctgcc ggacgtcgtg gacgtcaact cgctgggggg
    14401 ctttgtcaaa cagtaccagg tcatggtgga ccctggcctg ctgcggaaat acaaccttgg
    14461 attgcatgac gtcttcgcgg cagttcaaca aaacaatgcc aacgcaggcg ggaacattct
    14521 cgagaaaaac tccgagcgct atatcgtgcg aggtgtcggg ctcatcaaga ccgtggcgga
    14581 catcgagcag atcgtcgtga aggaagtcgg cggcacgccg gtttatgtcc gggacgtcgc
    14641 ggaagtgcgc atcggcagcg ccattcgcca cggcgcggcc gtgatcaacg gccaacagga
    14701 aaccgtcacg ggaatcgtgc tcatgcttcg cggcggaaac gcacgggaag tcgtgcaagc
    14761 cgtcaaaaca aaaatcgacg acatccatgc gcacaagctg cttcctgacg gactgcgcat
    14821 cgtgccgttt tacgaccgga ttgaattaat caccgcnnnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnttcc tgacggactg cgcatcgtgc cgttttacga ccggattgaa
    15001 ttaatcaccg ccgcgctggg tacggtctac aaggcgctgc tggaaggcat gctcctcgtc
    15061 gtcgtcgtgt tatttctgtt tctcggcaat gtccggagcg ccctgatcgt caccgccacc
    15121 ctactcttga cccccctcgc caccttcatc ctgatggacc gcgtaggact gaccgccaat
    15181 ctcatgtcgc tgggcggtct ggtcatcgcc ataggcatga tggtcgacgg ctcggtggtg
    15241 gtggtcgaaa atatttatcg gcatttgtcg gaacaccgtg actcgcgcga aggaaatgtg
    15301 tcgacgatcc tgcgggcggc gatggaagtg ggacagccgg tcgtgttcgg catcctgatc
    15361 atcatcatag tcttttttcc catcctttcc ctccagggca tggaggggaa aatgtttcaa
    15421 ccgttggcct acaccatcat catcgcgctc ctgatttccc tgcttctttc gcttaccctc
    15481 tcgccggtgc tctgttcact ggtgctcaaa gacgccaggg aggaagacac gctgctgttg
    15541 cgttggatta aacgcgcata catgcctgcg ctcggctggg ctctccgtca tcgcacggtg
    15601 gtcctcggct cggctctggt gatgctggcc gccagtctgg ccctgtttcc ttttctgggc
    15661 ggcgagttca tcccgatcct caatgaaggg tccgtctcac cgcagacgat tcgtcttccc
    15721 ggtatctcgc tggaaaaatc catcgagatc gagaaggaga tgcagcgagc gatcctggaa
    15781 ttcccggagg ccaggatggt ggtctccaaa atcggacgca ctgaactggg caacgaccct
    15841 caagaaccca actccagcga tccggtcgtg tccctcgtgc ctatggatca atggaaaacc
    15901 gccaccacca aggcgcaact cgatgacgcc atccggcgtc ggctgcaacg tattcccggg
    15961 gcgaatttcc tgatcagcca gccgatccag caacgagtcg atgaattggt ttccggagtc
    16021 cggtcggagg cgaccgtgaa ggtgcttggc gacgacctgg acgtcttgcg cgccacagcc
    16081 gataagatcc aaacgatcat gaccggcata gacggcgtaa aggacgtgcg ggtggaacag
    16141 ttattcgggc aaccctacct caccatcgac atcgaccgcg gccaaatcgc ccgacatggg
    16201 atcaacgtcg cccacgtgcg cgaaatcatc acgacggcga tcggcgcaga gcccgcgacg
    16261 cgcgtctatg aagggcagcg gcgcttcgac ctcatcctgc gctatcccga gcaatatcgc
    16321 gatagcgtgg acacggtgag caacattctt ctgaccaccc ctaccggggc gctcattccg
    16381 ctgggaaaca tcgccaaggt ggagttacgc gaggggccgg cgctgatcag ccgggaaggc
    16441 ctgcaacgga gaatctacgt cggcttcaac accttcggtc gcgacattga aagtatcgtg
    16501 catgaagcac agacgaagat tgcgcgacag gttccgctgc ccaccggcta tcggctgcaa
    16561 tggggcgggt ccttcgagaa catgcaacgg gccatggcgc ggttgaagat cgtggtgccg
    16621 ctgacgatcg gcgtcatctt cctgattctg ttctcctcgt tcaattcgct gcgccatgcg
    16681 gcgttgatta tcctgaacct tccatttgcc ctcatcggcg gaatcgccgc gctgtggctg
    16741 accggcgagt acctcagtgt gccggcctcc gtcgggttca tcaatctctt cggcgtggcc
    16801 gtcttgaacg gcatcgtgct tgtctcgtac atcaataaac tccgcgctga agggatggcc
    16861 ggtgaggaag ccgtggtcac cggttgctcg ctgcgcctgc gcccggtctt gatgacggcc
    16921 gtggtggcat tgttgggatt gctgccgctt gcactgtctc acgggatcgg gtcggaggta
    16981 caacgtccct tggctatcgt cgtgatcgga ggactcgtca gttccaccct cctcaccttg
    17041 atcgtcctcc ctgtgctcta ccaatggttc gatcggccgg acaaatcggc cgcccctgca
    17101 aattagacgt cgttctcttc gcgcatttgc tctatgtccc tagtgaaatg ccgaggtttc
    17161 ttactgcgga tggtcctgta tcatacgtag tctgaacttt tcccatctcg cgatggcgca
    17221 gggagcatgt ggattctatg gcatggacgt ttctgattgt ggccggactg tttgaaatct
    17281 gctgggccat cgggctgaaa tatacggatg ggttttcgaa attctggcca acggcgggga
    17341 cgttgatggc tatggccgcc agtttcggct tcctggctca ggccttaaaa agtattccgg
    17401 tgggaacagg gtacgcagtc tggaccggca tcggagcggc cggtaccgcc gtgttaggga
    17461 ttgtactctt cgcagagtcc gtctccatcg tgaaagtgtt ttctctcctt tgtatcgtac
    17521 tcgggatcat cgggctcaaa ggcagcgcct gaccacagag gtggcgccca ctctcactga
    17581 ggtctacagg tccatgttac ccgtcgccac ccccgaaccg caggagtcga cgctgttgcg
    17641 gtttccgaac cggaatacgc cgcctcggta tgaccgggcg atcggcgagt gggctttggt
    17701 cattcccctc ggaactgccg gactcttttt cgttcatggt catgagtggc tgcatgatct
    17761 ctcgaacccg cttcgattga gcgccatgct cgcctggctg ctgcttgtga tcgccttctc
    17821 cgcctttgcg gtgcttcgtc atgcagaaca tcttgccgcc cgccttggag aaccgatggg
    17881 gaccgtcatc ctgaccttgt ccgtgaccgg gatcgaggtc atgatgatcg ccgcctttat
    17941 gtatacgggt aatggcaacg cctcgttggc gagagatgcg atgtttgccg tggtgatgat
    18001 cgtgctgaac ggaatggtcg gcctgtcact ccttctcggc ggattgcgct atcacgaaca
    18061 gacgtacaat ttgcagggag ccaatgcctt tctcgccgtc atcgtcccgc tcgcggtgct
    18121 ggggctcgtc atgccgagtt atacgatttc ctccccgggc ccgacctttt caccacatca
    18181 agccatcttt ctcatcgtga tgtcgctcgg actctatggc gtatttctcg cgatccaaaa
    18241 cctccgtcat cgtaactatt tcgtggcgcc gcgcagacag agcagacgca acgcggcgat
    18301 cctgcatgaa caccagcctt ccccgggagg ctccgtgtgg tttcatacac tgctgcttct
    18361 ggcatacctg ctgccgctag taatcctctc agaacatgtg gcaacgccca tcgactacac
    18421 gctacgactt tggaatgctc ctcatgcctt gggaggtgtc ctggttgccg gtttggtgct
    18481 ggcgccggaa tcgctcggtg ccgtgcgggc cgcgctggcc aaccagctgc aacgttcggt
    18541 caatatcctt ctgggatcag tgctcgccag catcagcctc accattccgg cagtgctggc
    18601 catcggcttc tttaccgact cgacgatcct gctcggtatc gatccggttg ataccatcct
    18661 cctggtcctc acgtttgtgg taagcatgct cacctttgcc gtcagacgca cgaatgttct
    18721 ccttggcgcc gttcacctgc tcctgttcct ggcttatctc atgctgattt ttgagaaata
    18781 accgaaaaat cggtctccct ccgaataagc cgccgggttg atttgccgcg ccttcgcctc
    18841 ctaagatacg ggcgagaggc ggccagttac gccgggaatt gaaggccaat cagatccacg
    18901 ctggacaggt ttcacattcc gagttcctcg gcggattgtc agtgttttct gaaaagacac
    18961 gcatgtcagg cgccaaatca aaaacgttca cgatcctgct cgtcgaagac aacgcgggag
    19021 acgtcgatct attcttacgc acggtcgagc acgaactgcc ccgccatgaa gacgatgaag
    19081 tgacgctcgt cctcaacgcc acagccgaag gtgcgctcga acgtctgcgg cagcagccca
    19141 tcgatctcgt gatcgccgat gtgcgcttgc cgggcatgag cggtatcgaa ctccttcggc
    19201 aagtgcagga tttgaatcgg cgtatcccgg tcatcattct cagccgcgtc gacgccacgg
    19261 agaccgtcat tgatgcgatg cgtcacggcg cgttcgacta tatcgttgaa ccatacgact
    19321 gcatggatct ggcggcccgt atccaccgag ccatgcgcat gtcggaaatt cttctccagg
    19381 gctcgcccga cgaaccggct gcccctcgta tcccgttcaa aaaccttgtg ggcgtgagcc
    19441 ccgcgatcag aaacgtgatg gcgatgattg aagccatcgc gcgggttccg tcaaccacgc
    19501 tcattattgg agaaaccggc acgggcaagg aactgattgc gaaagcgatt catgagcgaa
    19561 gcctcgatag tgatgggccg tttcaagtcg tcgattgcac aaccttctcg gaaggcacag
    19621 tggagagtga actcttcgga cacgtcaggg gcgcatttac cggggccgtg gccgaccgaa
    19681 caggcttgat cgaatccgga agccacggca ccgtcttcct ggatgaaatc ggcgatctcc
    19741 ccgccactct ccaagccaag ctcttgcgag tcctggaggc tggtgaagtt cgtgccgtcg
    19801 gcagcaccca acagcggaag gtgaatgtcc gcttcgttgc cgcgacgaat caggacctgg
    19861 ccgaaaaggt gaagcggaat gagttccgca aagacctgtt tttccgcctc aacgtcatgg
    19921 tcatccgcgt gccgcctcta cgggaacgga cggaagacat tcctgttctt gcccgacact
    19981 ttatcgcccg ttatgccaag gagtttacga aacgggtgga cgatctccat cccgcggccg
    20041 tgacggaact ggtggcctat ccctggccgg gaaatgtgcg tgaactccgc aacgtgatgg
    20101 agcgagcggt catgctcgcc aagggggatc gtatcggtat tgcagatgtg gccgcgatgc
    20161 tcgccgttcc cgacgaacgg caacgagata ctcccgacga agattatctc catttgccct
    20221 acgcaaaggc gaaagaacag gtgctcgaag cgttcaaccg tcgatatatc tccacgaagc
    20281 tcaccatcca tcagggcaat gtcacgcatg ccgcccaaga tgctggacta cctcgttcat
    20341 actttcatga gatcatgcgc cggtattcca aggacgaaaa ataatggtgt cagtaaagca
    20401 tgacaccaaa agccgacata gatgcacaag ctattgtttt aaatcacgtt tcatgcatct
    20461 catgacgccg tccgcattta cagacactgc cttcatcttt aatatcaaca acttaagcct
    20521 ctgaactttc tccctagtca gtagttcctg accctgccta tcaggccttc cttccccatc
    20581 caactccctc gaaaacacca tcacgcaatc aattgatttt actgcattaa tcgtcactaa
    20641 attgccatcc aaagagcgcc agatttctcc cctttcggca tgtgaattgt aatactccta
    20701 taccgcgagc gaatgtccgc tcgaaatttt cgaattgtga gggacgaatg tttccaattc
    20761 gacacgcgga aagggggtac accagacgag attgcatcca cgtgagtgta gtgaagttgt
    20821 acgaacaggt gcatgaggga ctcatgcatc tcacaaggag gtagctatta tgcaggtttc
    20881 cgtttcacgg agacagtttc tcaaaatctc ggcgggcacc gtcgcggccg tggccgtggc
    20941 ggacaaagtc ctcgcgctga ccgcgctgca gccggtcatc gaggtgggga acccgctggg
    21001 tgagtacccg gatcggtcct gggagcgtgt gtatcacgac cagtatcgat acgactcatc
    21061 gtttacctgg tgctgctccc cgaacgacac gcacggctgt cgcgtccggg ccttcgtccg
    21121 gaacggcgtc gtcatgcggg tggagcagaa ctatgaccac caaacctatg aagacctcta
    21181 cggcaaccgc gggacgttcg cgcataaccc gcgcatgtgc ttgaaaggct tcaccttcca
    21241 ccgtcgcgtg tacggtccct atcgcttgaa gggcccgttg atgcggaagg gctggaagca
    21301 gtggatggac gacggctccc ccgagctgac gcccgatgtg aagcgcaagt acaaattcga
    21361 cagccgcttc ctggacgacc tcaaccgggt gtcgtgggat tcggccttca cctatgtcgc
    21421 gaagggcgcg attctcattg cgacgcgcta cagcggtgaa gccggagccc gtcggctccg
    21481 ggagcagggt tatgcgccgg aaatgatcga aatgatgaag ggcgcgggcg tgcgatgctt
    21541 caagcatcgc gcgggcatgc cggtgctcgg catcgtcggc aagatgatga acacccgctt
    21601 caacggcgga tgtttgccgt tgctcgactc ctggattcgg aaagtcgatg ccgaaaaggc
    21661 gcagggcggc aagtactact ccaactacac ctggcacggc gaccaggatc cgtcccatcc
    21721 gttctggaac gggacccaga actgcgacgt ggacctctcc gacatgcgct tctccaagct
    21781 gaacaccagc tggggcaaga acttcgtcga gaacaagatg ccggaagcgc actggaagct
    21841 cgagtccatc gagcgcggcg cccgcatcgt cgtcattacg ccggagtaca acccaacggc
    21901 ctaccgcgca gactactgga ttccgctgcg tccggagacc gatggcgcga acttcctcgg
    21961 tgctgcaaag atcatcttcg atgaaaactt gcaggacatc gactacatca aggaatttac
    22021 cgacctgccg ttgctcgtac ggacggatac gcttcagtat ctcgatccgc gcgatgtgat
    22081 tgccgattac aaattcccgg atttctcaaa gagctattcg ggccggattc aggccttgaa
    22141 gccggaacaa atcgaacggc tcggcggcat gatggtctgg gacctgggga aacagcaggc
    22201 cgtcccgcta cacagagagc aggtcgggtt ccacttcaaa gccagcggca ttgatcccgc
    22261 gttgaccggc acccatcgag tgaaattgct caatggccgg gaaatcgacg tgatgccgct
    22321 ctatcagatg taccaggtgc acctccagga ctacgacctg gataccacgc accagatcac
    22381 ccgggcgccg aaggacttga tcgtccggtg ggcgcgcgat tcgggcacag tcaagccggc
    22441 tgcgatgcat aacggcgaag gcgtctgtca ctatttccac atgacggaaa tgggtcgggc
    22501 ggcggcgttc attatgacca tcaccggcaa catcggcaaa ttcggcacgg gctgccatac
    22561 ctggtccggt aactacaagg ccgggatctg gaacgcggtg ccctggtccg gcgcgggcct
    22621 cgcggtgcat accggtgagg atccgttcaa cctgaccctg gatccgaatg cccacggcaa
    22681 agagatcaag acccgctcgt actactatgg tgaagaagtc gggtactgga accatggtga
    22741 tacggccttg atcgtcaaca cgccgaagta cggccggaag gtgttcaccg gcaagaccca
    22801 catgccgagc cccagcaaag tccgctgggt gaccaacgtg aacatcctca acaacgccaa
    22861 gcaccactat gacatggtga agaacgtcga tccgaacatc gagatgatcg tgacccaaga
    22921 catcgagatg acctcggacg tcaaccatgc cgacgtggcg tttgcgtgta actcgtggat
    22981 ggagttcacc tatccggaaa tgacgggcac ggtctccaat ccgtggattc agatctggaa
    23041 gggcggtatc cgtccgctgt acgacacccg taacgatgcg gataccttcg ccggtgtggc
    23101 agccaagctg tctgaaatga ccggggatgc gcgcttccgc ggcgtgttcc acttcgtgta
    23161 catgaaccgc gtcgatgtct atccgcagcg gatgctggat gccagcgcca cctgctacgg
    23221 gtacagcgcg gacgtcatgt tgaagtcgga aaagggctgg atggtcatgg gccgcaccta
    23281 tccgcggcac ccgctctggg aagagaccaa cgagtccaag ccccagtgga cgcggtcggg
    23341 tcgcatcgag acctaccgca tcgagccgga agccatcgaa tacggcgaaa acttcatctc
    23401 ccaccgggaa ggcccggagt gtacgccgta tctgccgaat gcgattttct cgaacaatcc
    23461 gttcattcgg ccggatgact acggcattcc gatcaccgcg cagcaccatg atgacaagca
    23521 cgtgcggaac atcaagctgc cgtgggcgga aatcaagcgg catccgaacc cgttgtggga
    23581 aaagggctac cagttctact gcgtcacgcc caagacccgg caccgggtgc atagccaatg
    23641 gtcggtgaac gactgggtgc agatttatga gtcgaacttc ggcg
//
LOCUS       DAOQDS010000059        21607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_918_length_21607_cov_21.206199, whole genome shotgun sequence.
ACCESSION   DAOQDS010000059 DAOQDS010000000
VERSION     DAOQDS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 21607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21607
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_918_length_21607_cov_21.206199"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..1171)
                     /locus_tag="PK589_13950"
     CDS             complement(347..1171)
                     /locus_tag="PK589_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ20759.1"
                     /translation="MPLRNGLSEELNRQGNEHFARGHYTDAYACYAKALECDRLTGDH
                     RALSATLGNLGNICAVSGRRESAQSYYQEVLELQKVLGDDKGIGTTLANLGNLRADAG
                     EWDRARAYYLEALDIMTRVHDELGKAVLFSDLGLVARETGQCDDALRYYEQSLVLMRR
                     LNNQGGVADAWRMMGRTFAIQKRYDDAIACCQTSQSIAERSRDELRAGGARYVLAQCY
                     EDLGQLQAAVDLLEQVVRMDRKYALPKLTENTARLASLRARLEAEDPSPQPRRSRA"
     gene            complement(1178..1774)
                     /locus_tag="PK589_13955"
     CDS             complement(1178..1774)
                     /locus_tag="PK589_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20760.1"
                     /translation="MKQLLPGIWQWSWFSDEKRLDFNGLFLTVGEHRIVIDPPPMTAE
                     ASTLIRRQGALDYIIVTNRDHLREAVAYQAEFRCQLQIPEADASQMDVKPTKTYKDGE
                     LLPGGIWAIQLKDQKSPGESALYIQQGKGVLIVGDALIGKPAGALSLLPSERYGDVAK
                     AKEGLRRLLKYNFDSILVGDGASIVFGAKQAVEQVLQA"
     gene            complement(1798..2037)
                     /locus_tag="PK589_13960"
     CDS             complement(1798..2037)
                     /locus_tag="PK589_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20761.1"
                     /translation="MDEWEEGKLMFLEVVKGIDQGVEVVIPTKPTNSMFLISLTKGAN
                     RKFITVSEDDIIDLPTEAGIRTKVTKTLQDAVATL"
     gene            complement(2055..2597)
                     /locus_tag="PK589_13965"
     CDS             complement(2055..2597)
                     /locus_tag="PK589_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNJ20762.1"
                     /translation="MKRVFIVTALVLLPLWAAHIAMAAGFFKVGEPAPAFALSSITGD
                     AVSLEQLKGKVVVLGLFHICDPCMMQGTNLQKVHEVLAGKNVAVVGINSSGNSKRDVG
                     EFLSAFPVKVTYPYLLDPNKVTDKLYGGGKFIPNVYVIDQRGVIRWQRVGNMELAGAE
                     VIVQEVEKLLASAPAGSGPM"
     gene            complement(2786..3934)
                     /locus_tag="PK589_13970"
     CDS             complement(2786..3934)
                     /locus_tag="PK589_13970"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNJ20763.1"
                     /translation="MSEFVRVEITDLVCDGINWDLLWQLSKAHGVAPLVYRNLAAICP
                     AAVPSAIHEAYRRQNQSTTLMNTVLAKELVALLDALAAKGVMAIPFHGVTLAQVAYGD
                     LSMRECEDIELIVEHGAVSQARKVLWSHGYQLLGADNDAEADPREGHHVFLKRNGMAM
                     VKLQWGLAGYHSNFRLDRHALWGRLKPVGLPTKAVMGLCPEDLLILLCVHGTLHNWKQ
                     LKWVCDIAEFVRRKPALDWSRILFQASEWRCRRMVLLGLAMAQNLFDIVLPRTVSHEI
                     ESDTDLPVLVRRMPQQLLKHPDQGIDEDCADAVSLTVKDSVWARWKLGFILCRVDAEV
                     IHRPLPWFRFQRQLRLMSACMRPLLRSVPKEIVPVRVRHMVVGWLQSS"
     gene            complement(4219..4536)
                     /locus_tag="PK589_13975"
     CDS             complement(4219..4536)
                     /locus_tag="PK589_13975"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNJ20764.1"
                     /translation="MPATVPVLSERTIDFSQMFPHQNSDVRITNQGGESLLFNVRTGH
                     FHRLNALGSAVWLQCTGTQSVARILSTISETFDVTATQAQSELLDVLVQLQDDGFLRI
                     EKR"
     gene            complement(5085..6515)
                     /gene="purF"
                     /locus_tag="PK589_13980"
     CDS             complement(5085..6515)
                     /gene="purF"
                     /locus_tag="PK589_13980"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNJ20765.1"
                     /translation="MAKELPLIASDQFHDECAVFGIYGHKEAANLAYLGLYALQHRGQ
                     EASGIVSNDGEQFYVEKGQGHVADIFSQQALARLPGAMAIGHNRYSTAGGAGLKNVQP
                     LSVNFAFGNLAVAHNGNLINATMLRSELEAYGAIFQSTSDTEVIIHLIAHSRADTLLD
                     RVIDSLTQVRGAFSVVLMTDQGVIAARDPHGFRPLCLGRFRDAWIVASESCAFDLLDA
                     EYVREIEPGELVVLDHRGVTSYKPFPPTQPAMCVFEYVYFARPDSRIFGGSAVYSIRK
                     AFGRQLAQESAVDADIVIPVPDSGVPAALGYSEGSGVPFESGLIRNHYVGRTFIEPEQ
                     SIRHFGVKVKLNAVPEVLAGKRVVVVDDSLVRGTTSRKIVKMLRHAGAKEVHMRISSP
                     PIVSPCFYGIDTPTKKELIASSHTTDEIRKYITADSLAYLSLDGMVKAAPGTPSQYCN
                     ACFTERYPISFTRAEELQLGLFEPPG"
     gene            complement(6528..8774)
                     /gene="purL"
                     /locus_tag="PK589_13985"
     CDS             complement(6528..8774)
                     /gene="purL"
                     /locus_tag="PK589_13985"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNJ20766.1"
                     /translation="MKSLVITQAILEQHKLSEEEYKKILEILGREPNWTELGMFSAMW
                     SEHCSYKSSRIHLKKLPTTGPRVVQGPGENAGAVDIGDGLCAVFKMESHNHPSFIEPY
                     QGAATGVGGILRDIFTMGARPIALLNSLRFGGLDNANTRHLLKGVVSGIAGYGNCMGV
                     PTVGGEIVFNDIYALNPLVNVFCLGIARKDKIFLGKAAGVGNPVIYFGSKTGRDGIHG
                     ATMASDSFDEQAAQKRPTVQVGDPFTEKLLLEACLELMAGDCLVGIQDMGAAGLTSSA
                     CEMASREGTGVELELADVPRREPGMTPYEIMLSESQERMLMVAKAGREDEVIKICRKW
                     DLDVAVIGRVTDTGKVVLKENGQIVAEIPAKALADDGPRYDRPSAPPAYQEMLQSLNY
                     DALPDVKDANATLLELLGAPTIASKRWVYEQYDHMVRTNTMVRPGSDAAVLRVKGTNK
                     ALALSVDCNGRYCLLNPYEGAKIAIAECARNLACSGAEPIGVTDCLNFGNPQRPEVMW
                     QFVLAIEGLKDACEALSVPIVSGNVSLYNETKDLSIYPTPMIGMVGLIEAADRTATQW
                     FKQAGDVIILLGRTREDLGGTEYLKIVHHREQGSPPLLNLEDERAVQACTIQLIREGL
                     VQSAHDCSDGGLAVALAECCVSGPNGHVGAVVQLPLDGLRRDALLFGESQSRIVLSVK
                     QEQVDNVLSAARNAGVPASRIGVVGGSRLVIRVDGDQRVAGCTIDLEVTSVQERWALA
                     IPRALGQD"
     gene            complement(8842..9564)
                     /gene="purQ"
                     /locus_tag="PK589_13990"
     CDS             complement(8842..9564)
                     /gene="purQ"
                     /locus_tag="PK589_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNJ20767.1"
                     /translation="MNIGVVVFPGSNCDHDCEYIFKDVLGQSVKMIWHKDTLPAGLDA
                     VVLPGGFSYGDYLRTGAIARFSPVMGAVKTFARDGGLVIGICNGFQILLEAGLLPGVM
                     LRNTSLNFICKDVYVKVENAATRFTNRCESGQVLKIPIAHADGNYYTDPVTLGGIKAN
                     AQVIFRYCTAEGKITPEANPNGSLDNIAGIMNADGNVLGMMPHPERSAETLLGNEDGR
                     LIFLSMLSAFGKSEQPSRLVGV"
     gene            complement(9682..9921)
                     /gene="purS"
                     /locus_tag="PK589_13995"
     CDS             complement(9682..9921)
                     /gene="purS"
                     /locus_tag="PK589_13995"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNJ20768.1"
                     /translation="MKAKIHVTLKQGILDPQGKAIEHALDSLGFKNAANVRVGKYMEV
                     DVNETDSAKADAQVKQMCEKLLANTVIEEYRYELQ"
     gene            complement(9918..10142)
                     /locus_tag="PK589_14000"
     CDS             complement(9918..10142)
                     /locus_tag="PK589_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20769.1"
                     /translation="MNRFLRFFLDYGLVLAILVWAATVALMAYHLDESPWRWAFILLS
                     MAGLGTIGVIFWIRKYVNKTAQTQRVGKVQ"
     gene            complement(10262..10975)
                     /locus_tag="PK589_14005"
     CDS             complement(10262..10975)
                     /locus_tag="PK589_14005"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNJ20770.1"
                     /translation="MTGEPAGTMLYEGKAKKIFTTERPDQVLQYFKDDATAFNAQKRG
                     TIVDKGVVNNKVSERLFKLLEQQGIQTHFIERVSDREMLTKKVTIVPVEVVVRNVVAG
                     SLAKRLGLKEGAAIEPAIIEFYYKDDALGDPLVNDDHLRVLNVATPAVLREMRELGHK
                     INAVLLPFFKERRMLLVDFKLEFGVFHNRLILADEISPDTCRFWDQATKESMDKDRFR
                     KDLGKIEEAYQEVLKRVCE"
     gene            complement(10990..11787)
                     /locus_tag="PK589_14010"
     CDS             complement(10990..11787)
                     /locus_tag="PK589_14010"
                     /inference="COORDINATES: protein motif:HMM:NF018484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HNJ20771.1"
                     /translation="MMAALIMRIVLMVALVFGAAVSPVGATEREALLTQPGVYGTFAA
                     FQMDHDWWDLNGEARVIAVSEVKGLIEQFSQKIVIESYLLRGLSDHADLMFRIHAHAL
                     SDTQQFLTSLLGTRLGRHLVATTYLHGMTKPAAYAAGFPESMKQALQSPAPETGTKSY
                     AIVIPIRKDADWWALDQDSRARLMQEHTQAALPYINTVKRKLYHSSGLDDFDFITYFE
                     TDKLEEFHGLIRALEQVKEYRHDRRFGHPTLLGTIRPLDDILELFAR"
     gene            complement(11784..>12017)
                     /locus_tag="PK589_14015"
     CDS             complement(11784..>12017)
                     /locus_tag="PK589_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNJ20772.1"
                     /translation="RKESYEAVQRNAMTAWKGGVGFQELVNRDPFITTHLKPSEITAC
                     FDPMYYLRHLDQVFRRVFGARAIPAARRRKRRP"
     assembly_gap    12018..12117
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12118..12713)
                     /locus_tag="PK589_14020"
     CDS             complement(<12118..12713)
                     /locus_tag="PK589_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNJ20773.1"
                     /translation="MIERYTRPRMSAIWDLTHKYEIWLEVELQACAAFERAGQIPRGT
                     SAKIRKKAKIDVARIAEIEKVTKHDVIAFLESLMASVGPEHRYLHMGLTSSDIVDTSL
                     AVQMTEALDLILEDLDELIAVLKKRALEHRHTVMVGRSHGIHGEPVSFGFKLAIWYEE
                     ACRHRTRLEAARKDIAVGKLSGAMGTFAHQGPEIEEYV"
     gene            complement(12810..13262)
                     /gene="nusB"
                     /locus_tag="PK589_14025"
     CDS             complement(12810..13262)
                     /gene="nusB"
                     /locus_tag="PK589_14025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNJ20774.1"
                     /translation="MASRHQARERAVQILFQYDIHGQAGPWLDDFWTQYPLNDELRVF
                     AERLVAGVREHKKELDALIGTHATNWKVGRMPIIDRNILRLGCFELLFVPEVPAKVTL
                     NEAIELAKSFGDEEASKFVNGILDKVLGSDSRLERKRAFPELPVVGSD"
     gene            complement(13276..13815)
                     /gene="ribH"
                     /locus_tag="PK589_14030"
     CDS             complement(13276..13815)
                     /gene="ribH"
                     /locus_tag="PK589_14030"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNJ20775.1"
                     /translation="MKLRKGSQDATGLTVGIVASKFNKFVTSRLLTECVKALTKAGAS
                     DEAIEVVRVPGAFEIPLVARQLARSGRFDAVICLGAVIRGDTPHFEYISTEVSRGILQ
                     ASMESNLPVIFGVLTTDTVAQAIERADPAKLNRGADAAKTAIEMVNVMRQLKKEESED
                     VPPGPAPRRAGRTAARAKR"
     gene            complement(13965..15173)
                     /locus_tag="PK589_14035"
     CDS             complement(13965..15173)
                     /locus_tag="PK589_14035"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNJ20776.1"
                     /translation="MATPFNSIEEAIRDIKKGKFIILVDDEDRENEGDLVMAASKVTP
                     QAVNFMAKHARGLICLALTPERVEELQLAPQAAENTATFGTAFTVSIDARKGITTGIS
                     AADRATTIHVAIDPRCSPADLARPGHIFPLKAQVGGVLRRAGQTEGSVDLARLAGLYP
                     AGVICEIMNEDGTMARVAELTKFAKRHKLKMVTIKALIEYRMHRETFVRRAASAFLPT
                     TFGDFEAIAFENDIDGATHIALVKGQVDADTPMLVRVHSGCLTADVLGSLRCDCREQL
                     HRAMEIIQKDGRGVLLYLNQEGRGIGLLNKIRAYGLQDSGSDTVEANLQLGFKADLRD
                     YGVGAQILVQLGLHKIRLITNNPRKIVGIEGYGLKVVERVPIEIAPRADNEKYLRTKK
                     AKLGHLLKKV"
     gene            complement(15238..16479)
                     /locus_tag="PK589_14040"
     CDS             complement(15238..16479)
                     /locus_tag="PK589_14040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ20777.1"
                     /translation="MKSLGNVRTLGTALRSGHWPTLAGAWLHLTVSFMVWVLFGALAV
                     PMGAELHLTATQQSVVVALPLLSGALLRIVTGWACDWSGAKRTGLWVLGLQLIAVLWA
                     ALSGTQYVELLAIALLLGAGGASFAVAMPVASRAYPAAHQGLVLGLVASGNIGTVLVL
                     LLAPQWEGQLGWHGACGIMALPILLAIGLFARVVREEPHTISVAEDAWWQQAWGMVQV
                     PSVYWLCLVYAITFGGFVGLTSFLPVLLHDQYGSDPIVGGAIAALCGLTGSLIRPVGG
                     YVADRWGGLPALAGVFMVLAAVTAFAASLPLLPWLASLLMVTIAVMGFGNGVIFQIVS
                     EWFPKDIGLASGLVGAAGGIGGFFVPIGFGLLREAMGTFVPGFLGLALVSAAAAMTVG
                     SALRWRPRAVASATNAEGFSR"
     gene            16648..17679
                     /locus_tag="PK589_14045"
     CDS             16648..17679
                     /locus_tag="PK589_14045"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HNJ20778.1"
                     /translation="MRQRKPKHNTDSDSDSAAELDEDDSLEPEALEPTDEEVTEEHEA
                     AERQPSKESPSAPLSTSLVPVTTLQQYLTEIRRYPYLSKEEELRLFEEYQLRGSRDAA
                     MKLILANLRVSVSIASEYLHTGADHMDLIQEGNVGLLQAIKKFDPTKNVRFYAYAAWW
                     SRAYILRYLLNTYRLIKVGTTQDQRKLFYNLKKEKAKLEREGFAPDSKLLADRLNVRE
                     RDVIEMDQRLGSWELSLDQPIGQEHDGTLMDVLPAHEQPADEQLADTQLKALFRRKLA
                     EFTKTLDEREEDILRNRILSETPLTLEDMGAKYGITKERTRQLEARIIKRLRDFIKKD
                     VKDFDRLRM"
     gene            17660..19219
                     /locus_tag="PK589_14050"
     CDS             17660..19219
                     /locus_tag="PK589_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20779.1"
                     /translation="MTVSGCKTGASIRIVLTILMLTTLVESGCPRPSHAASLITQENA
                     RPGQSDWVLTDPADHEVEGYASATSIQRGEPLRLYIHSTDPHVTVDIYRMGWYGGTGA
                     RLVRGGITLPGARQPMPTPDPVTGLIECRWQVSYTLTTETHEKNDWLSGVYLAKLTGS
                     ASGKQSYIIFVVREDDRPSEFLFQSSVTTFQAYNNWGGKSTYPSNSRDEQWARKVSFN
                     RPYARSQHPQGGSGIGAGEFLTAMSIHPTRALSTAGWEYNMVRWLERHGYDVTYSTDI
                     DTHTRAEFWKGHKTWLSVGHDEYWSAEMRRHVEEARDHGVGLGFFSANTCYWQIRLEP
                     SLLTGEPNRTMVSYKEVAWNEDPLALDGDPSNDQLITVQWRESPVQRPEQALLGVMYG
                     TVPVDGDILITQPEHPLFNGVDLPPDHRLPGLLGYEIDQVFANGPAGLTVLAHSSYPR
                     DGEAVYGDMTLYQAQSGAFIFAAGTIQWSWGLDDYNAPTLRTARSSQAAQRITGNILR
                     LLASPASSPAH"
     gene            19533..19835
                     /locus_tag="PK589_14055"
     CDS             19533..19835
                     /locus_tag="PK589_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNJ20780.1"
                     /translation="MATESKEKKSTAAKNFQPLGDRLFVTYTEEMERTSGGIYVPDSA
                     KEKPQRGIVQAIGKKVENIKVGDQVLFDKYSGSKLRIEDEECLILKEEDILGIFTH"
     gene            19882..21525
                     /gene="groL"
                     /locus_tag="PK589_14060"
     CDS             19882..21525
                     /gene="groL"
                     /locus_tag="PK589_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247953.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNJ20781.1"
                     /translation="MMAKQLLYSEAARAAILRGVNQLADAVKATLGPKGRNAILDKKF
                     GAPTITKDGVTVAKEVELKNPYENMGAQLVREVASKTSDTAGDGTTTATVLAQAIYRE
                     GVKNITAGANPMEIQRGINKAVEVVIGELKKLSKPCQNKTEISQVGTISANNDKTIGD
                     LIAEAMEKVGKDGVITVEEAKSMTTSLDVVEGMQFDRGYISPYFVTNAERMEAVMDEP
                     LILINEKKVSSMKDLLPVLEQVAKLGKPLIIIAEEVEGEALATLVVNKLRGTLNVSAV
                     KAPGFGDRRKAMLEDIAILTGGQVISEDLGLKLENVKLTDLGRAKRVTIDKDNTTIVE
                     GHGDPKKIEGRVKQIKAQIEETTSDYDREKLQERLAKIVGGVAVINVGAATETEMKEK
                     KARVEDALHATKAAVEEGIVPGGGTAYLRCLKGLDSLKDLPLEQKVGVDIVRRSLEEP
                     VRQIAANAGAEGSVVVGRVREDKNPNGGYNAAADEYVDMIKTGIIDPTKVSRCALQNA
                     ASVAGLMLTTEVMITELPEEKKEPAGGHAGHNHGMEGMY"
BASE COUNT         4920 a         6528 c         5632 g         4427 t
ORIGIN      
        1 gtgatggcga catattcgtc gttcatttcc gtctgctcag cgaaccctgc cttgaggaga
       61 gcagggcgtt tcttggatac agcaggaccc agaaaatact tagcctgctt ggcgacttca
      121 tcggttccca aatcttccgt cgtcccgtca cacatcaaac tctttacatc atccatggag
      181 attccatgct gacaaaggaa ctgaccgtca gacctcagtt ctaagatcca tccatcatcc
      241 cctaaatcaa gaacgagggg gccgcctggc tccagttcgt agaattgggg aatactggtg
      301 cgtagctttg cacgagccgc ctcccaggct ggaagtgcca ccgtactcat gcgcgcgacc
      361 gccggggttg gggactggga tcttccgctt ccaggcgagc acgcagactt gcaagccgag
      421 ccgtgttttc ggtcaatttc gggagagcat atttacgatc cattcgcact acttgctcca
      481 gtaaatctac agcagcttgt aactgtccga gatcttcata acactgggcc aagacgtacc
      541 gcgccccgcc ggcccgcagt tcgtctcgac tgcgttcagc aatggattga cttgtttgac
      601 aacaggcaat cgcatcgtcg tatcgctttt ggattgcgaa agtccgcccc atcatccgcc
      661 aggcatcagc cacgccgcct tgattattca gccgacgcat caggaccagc gactgttcat
      721 agtagcgcag tgcgtcatcg cactgtccgg tttctcgggc taccaagccg aggtcggaaa
      781 acagcacagc cttgccgagt tcatcatgta ctcgggtcat aatgtcgagg gcttcgaggt
      841 aataggctct ggcgcgatcc cactcccccg catctgcacg cagattcccc agattggcca
      901 atgtcgtccc aatacctttg tcgtcgccta acaccttttg taactccagg acttcctggt
      961 agtagctctg cgccgattcg cggcgtccgc tgaccgcgca aatatttccg aggtttccca
     1021 aagtggcact cagcgcccgg tggtcacccg tcaatcgatc acattccaag gcttttgcat
     1081 agcaagcata ggcatccgtg taatgccctc gagcaaagtg ctcgttgccc tggcgattca
     1141 gttcttcgga cagtccgttt cgaagcggca tgatccttta cgcctggagc acctgctcca
     1201 ctgcctgttt ggctccgaat acaatgctag ccccatcccc cacaagaatc gagtcgaagt
     1261 tatatttcaa caaccggcgt agcccttcct tggccttcgc aacatcccca tatcgctcag
     1321 agggcaacag actaagtgcc cccgctggct ttccaatcaa ggcatccccg acaatcaaca
     1381 cgcccttccc ctgctgaata tagagggccg actcccctgg tgacttctga tccttgagct
     1441 gtatcgccca aatgcctcca ggcaacaact cgccgtcttt ataggttttc gttggtttca
     1501 catccatctg ggacgcatca gcctcgggta tctgtaattg gcaacgaaac tccgcctggt
     1561 aggcgaccgc ctcacggaga tgatcacgat tcgtcacaat aatgtaatcc agcgcccctt
     1621 gccttcggat cagcgtactt gcttcggccg tcatgggagg gggatcgata acgatccggt
     1681 gttcgcccac tgtcagaaac agtccgttaa aatcaagccg cttttcgtcg gagaaccagg
     1741 accattgcca aatgccgggg agtaactgct tcacgcgttt cgcctcatct gcagggttta
     1801 caaagttgcc acagcgtcct gcagagtttt cgtgaccttc gtgcggatac cggcttcggt
     1861 cggaagatcg atgatatcgt cctctgacac agtaatgaac ttacgatttg caccttttgt
     1921 cagagagatc aggaacatgc tattggtagg cttcgtcgga atcaccacct ccacgccttg
     1981 gtcgatgccc ttgacgactt ccagaaacat caatttaccc tcttcccatt cgtccatgga
     2041 tcctctttct cccgctacat gggacctgaa ccggcaggcg cagaagccaa taatttctcg
     2101 acctcctgaa caatgacctc ggcgcctgct agctccatat tgccgaccct ctgccatcta
     2161 atcacgccac gttgatcgat tacatacacg ttcggaataa attttccgcc gccatagagt
     2221 ttgtccgtca ccttattggg atcgagcaga tacggatagg tgacttttac ggggaacgcg
     2281 ctcagaaatt caccgacgtc ccgcttggag ttgcccgatg aattaatacc aaccacggca
     2341 acattcttac cggccagcac ctcatgaacc ttttgcagat tggttccttg catcatacag
     2401 gggtcacaaa tgtggaacag acctaggacg accacctttc cctttaactg ctcaagagaa
     2461 acggcatctc ccgtaattga cgagagtgcg aacgcgggcg ccggctctcc caccttgaag
     2521 aatccagcag ccatggcaat atgagctgcc caaaggggca acagcaccag tgcagttacg
     2581 atgaagaccc gtttcattcg cgcctccttc cgcaaggcca gccagcggga aaagccactg
     2641 cctttcctcg ctgatgcaca gcataatccc tcatgaatga ccatgctggc atcctagcac
     2701 gcgaattttt tcgggagcaa ccacttaccc aggaaagggc gcaggctgac taaacaactg
     2761 cctgactgag gaaggaccga gacgatcaag aactctgcaa ccagcccaca accatgtgcc
     2821 gtacacgaac ggggacgatt tctttcggca cgcttcgaag aagtggcctc atacatgccg
     2881 acattagccg aagctgtctt tggaaccgaa accacggaag gggacgatgt atcacctctg
     2941 cgtcaactcg acagagaatg aaacctaatt tccatcgcgc ccatacggaa tctttgaccg
     3001 tgagagacac ggcgtccgca caatcttcat caatgccctg gtcgggatgc ttgagtaact
     3061 gttgaggcat tcttcgcacg agcacaggca ggtctgtatc tgattcaatc tcatggctta
     3121 ccgtgcgagg cagcacaata tcgaaaagat tctgcgccat agcgagaccg agaaggacca
     3181 tccttctgca tctccattcg cttgcctgaa acaggattcg actccaatca agcgccggtt
     3241 tccgacgcac gaattcagcg atatcgcaca cccatttcaa ctgcttccaa ttatgtaacg
     3301 ttccgtgcac acacagaaga atcagcaagt cctcaggaca gagccccatc accgccttcg
     3361 tcggtaaacc caccggcttc agccgacccc acaatgcatg acgatcaagt cggaaattgg
     3421 agtggtagcc ggcaaggccc cattgcagtt tgaccatggc cattccgttt ctcttaagga
     3481 aaacgtgatg accctcacga ggatcggcct cagcatcatt atcagcccca agaagttggt
     3541 atccgtgcga ccaaagaact tttctggctt gagaaactgc tccatgctca acaatcagct
     3601 caatgtcttc gcactcgcgc atgctcagat cgccatacgc aacctgagct aatgtaaccc
     3661 cgtggaaggg aatcgccatg actcccttcg cggcaagcgc gtcaagcagc gccacaagtt
     3721 ccttcgcaag cacggtgttc atcagagtcg tagattgatt ttgccgccgg taggcttcat
     3781 gaatagccga cgggaccgcc gcagggcaga tggcagcaag atttcgatat accagcggtg
     3841 cgacaccatg agccttcgac agttgccaca ataggtccca attgattccg tcacacacta
     3901 ggtctgtgat ttcaactcga acaaattcac tcacagcggt tcgtgcgcag aggacgagca
     3961 agcggccctc gtcactcatt gcggagattc ctggacgtgg cggcttcgac ttgcgaaaag
     4021 cctctacccg acgtgatggt ttccatcgag cgatcgtaga tcttgtctgc tgtcccattg
     4081 gaatcctaac tcctgctgag cgttttaccc gaagaatagt gaggacctac ggcggatatg
     4141 cgacgtcggc gcgcacaata actcatgatt gaccggttcg cttgcacatt cagatggatc
     4201 cagctgctct atggatagtc atcgtttttc aatacggaga aatccatcat cctgcagctg
     4261 gacgagtaca tcgagcaatt cagattgggc ctgcgtagct gtcacatcaa atgtttcgga
     4321 aatggtggag agaatccgtg caaccgattg agtgccggta cattgcagcc ataccgcgga
     4381 tcccaaggca ttcaatcgat ggaaatggcc cgtgcgcaca ttaaacaata ggctttcgcc
     4441 gccctgattt gtgattcgca catcagaatt ttgatgcgga aacatttgtg aaaaatcgat
     4501 tgtgcgttca cttaaaacag gtactgtagc gggcatagag aacctcctga ctcagagggg
     4561 gcaacacatc gaccagtgca aaggttgcgg gttcatccat gacgcgaatt tgggaatcta
     4621 gtgcgccgaa cccggatgta gcatgccgat gcggcatgta ggcaaaggta gccgtagcag
     4681 ggcggtttct ctattcccag tgaggttggt gcaacatctc tttggagggg aacacagagc
     4741 ttttatggca gtccgataga gagggacgac aatgcccctt cctttgagct gatgagaacc
     4801 gggatctttg gagagactct ttggcccgta ggcacattgg tctcgtgaga ccagtcaata
     4861 acccacagcg gacccaatgc gatggaaaag gtattcggga agggaaagcc cagtgcgaag
     4921 gatgacgatc gacggaccaa acgtcagcgc tgctgtaatt acttgcgaaa aggaatcaga
     4981 cccgatgaat tcaccttcat gaacccggga gtgctgctct atgcatgcgc ataaggctac
     5041 tgtgacctca ggggcgagcg taacacgcga caagattttt accgtcagcc tggcggctcg
     5101 aacaacccga gttgaagttc ctcggctcgg gtaaaagaaa tgggatatcg ttcagtgaag
     5161 caggcattgc aatattggct aggtgtacca ggcgcagcct tgaccatgcc atcgagactc
     5221 aaataggcga gactatcagc ggtaatatat ttccgaattt cgtcggtggt atgactcgag
     5281 gcgatcaatt ctttctttgt cggcgtatca atgccataga agcacgggga cacgatcgga
     5341 ggagaactaa tccgcatgtg cacttctttg gccccagcat ggcgcagcat tttgacgatt
     5401 ttccgactcg tcgtcccgcg caccaacgaa tcatccacca caacgacacg ctttcctgca
     5461 aggacttctg gaacagcatt gagtttgact ttgaccccga aatggcgaat cgattgctcc
     5521 ggttcgatga acgtacgccc cacatagtga ttccgaatga gcccgctttc aaatggaacg
     5581 cctgaacctt cggaataccc taacgccgcc ggcactcccg aatctggcac gggaatcaca
     5641 atatccgcgt ccacagctga ttcctgggct aattgccggc cgaaggcttt tcggatcgaa
     5701 tagaccgcgc tcccaccaaa aatacgacta tcggggcgcg caaaatagac atattcaaac
     5761 acacacatag caggctgagt cggcggaaaa ggcttataac tcgtcactcc ccgatgatcc
     5821 aggactacca attctcccgg ttcgatttcc cgcacatatt ccgcgtcgag caaatcaaac
     5881 gcacagcttt cggaggcgac aatccaggca tcgcgaaagc gccccaaaca gagcggacgg
     5941 aatccatgcg gatcgcgagc cgcgatgaca ccctgatcgg tcatgagcac cacggaaaat
     6001 gcgccgcgaa cctgagtgag tgaatcaatc acccggtcaa gcagcgtatc cgctcgtgag
     6061 tgcgcaatca ggtggatgat gacttccgta tcggatgtcg attgaaaaat ggcgccatag
     6121 gcttccaatt cgctgcgaag catggtggca ttgatgaggt ttccattatg cgcaaccgcc
     6181 aagttcccaa acgcaaagtt gacgctcaag ggctgcacat tcttcaggcc agcgccgccc
     6241 gctgtggaat agcggttgtg tccgatggcc atggcaccgg gaagacgagc taacgcctgt
     6301 tgcgagaaga tatcggccac atggccctgc cccttctcga cataaaattg ctccccatcg
     6361 ttggagacaa tgccggaggc ttcctgcccg cggtgttgga gcgcgtagag accgagatag
     6421 gcgagattgg cggcttcttt gtgcccgtaa atgccaaaga ccgcacactc gtcgtggaat
     6481 tgatcggaag caatgagagg taattctttg gccatgacgt ctctgctcta gtcctgaccc
     6541 agcgcccgtg gtattgccaa ggcccagcgt tcctgaacgc ttgtcacctc gagatcgatc
     6601 gtgcaccccg cgacacgctg atcaccgtca actcgaatca ccaaccggct gccgccgacg
     6661 acgccgatgc gtgacgcagg cacgccggca ttcctcgcgg cactcaacac gttgtccacc
     6721 tgttcctgct ttactgatag tacgatccgc gattggcttt ctccaaagag caaggcatcg
     6781 cgccgcaaac catcaagcgg caattgtacc acagcaccga cgtgcccatt gggacccgag
     6841 acgcaacatt cggccagggc tacggctaat cccccatcgg agcaatcgtg tgccgattga
     6901 accaagccct ctcgaatgag ttgaatcgta caggcctgca ccgctcgttc atcctcgaga
     6961 ttcagcaacg gcggcgatcc ttgttcgcga tgatgcacaa tcttgaggta ctccgtcccc
     7021 ccaagatctt cacgtgttcg acccagcaag atgatgacgt cgccggcttg cttaaaccac
     7081 tgcgtggccg tacggtcggc ggcctcaatc aatcccacca tcccgatcat gggtgttgga
     7141 tagatcgaca ggtctttggt ctcgttgtac agacttacat tcccgctgac gatgggcacg
     7201 ctcaaggctt cacaggcatc cttgaggcct tcgatggcca gcacgaactg ccacatgact
     7261 tccgggcgct gcggattgcc gaaattgagg cagtcggtga caccgatcgg ttctgccccc
     7321 gaacaggcga gattacgtgc acactccgca attgcaatct tggcaccttc atagggattc
     7381 aacaaacaat agcgtccatt acaatcgacg gacaatgcca aggccttgtt ggtgcctttc
     7441 accctcagca cagctgcatc agatccgggc cgcaccatcg tgttcgtccg caccatatga
     7501 tcgtattgtt cgtagaccca gcgcttactg gcgatcgtcg gtgcgcctaa gagttcaagc
     7561 agagttgcat tcgcgtcctt cacatccggc aatgcgtcat aattcaacga ctgcaacatt
     7621 tcctgatagg ccggtggcgc actgggtcga tcatagcgtg ggccgtcgtc cgccagggcc
     7681 ttggcgggaa tttccgccac gatctgccca ttctctttca acacaacctt gccggtatcc
     7741 gtgacgcgcc cgatgaccgc cacatccaga tcccacttac ggcaaatctt gatgacttca
     7801 tcctcgcggc cggctttcgc aaccatcaac atgcgttctt gtgattcgga cagcatgatt
     7861 tcgtacgggg tcatgccggg ctcgcgacgc ggcacatccg ccaattccaa ttccacgccg
     7921 gtgccttccc gcgaagccat ttcgcaggcc gaactggtca aacctgccgc tcccatgtcc
     7981 tgaatcccga ccaaacagtc cccggccatt aactcgaggc aggcctccag cagcagcttc
     8041 tccgtaaacg ggtcgccgac ctgcacagtc gggcgctttt gggcagcctg ttcatcgaaa
     8101 gaatcggacg ccatggtcgc gccgtggatc ccgtcccgcc ccgtctttga gccgaaatag
     8161 atgacgggat tcccgactcc cgccgctttg cccagaaaaa tcttatcctt cctggcaatc
     8221 cccaggcaga agacattgac caacggattg agcgcataga tgtcattaaa cacgatctca
     8281 ccgccgaccg tcggcacccc catgcaattg ccataaccgg cgatgcctga caccacgcct
     8341 tttagaagat ggcgagtatt cgcattgtcc aggccgccga atcgcagcga gttcaacaga
     8401 gcgatcggcc tggcgcccat cgtgaaaata tcgcgaagaa ttcccccgac gccggtcgcc
     8461 gcgccctggt agggttcgat gaaggaggga tggttgtgag actccatctt gaagaccgca
     8521 cagaggccgt cgccgatatc gacagccccg gcattctcgc ccggcccctg gacgacgcgc
     8581 ggaccggtcg tcggtaattt tttcaaatgg atgcgggagc tcttgtagga gcaatgttcg
     8641 ctccacatag ccgagaacat gcccagttcg gtccagttcg gctcccggcc gaggatttcc
     8701 aggatcttct tatattcctc ctcgctcagc ttgtgctgct caaggatggc ttgggtaatg
     8761 accaaagatt tcatgtcttg cacattctct atcgtggatg agaaccggtc gatccggcgc
     8821 tcctattcat tcgccatgcc ttcacacgcc gactaatctg gatggctgtt cagacttgcc
     8881 gaacgcgctc agcatggaga ggaaaatcag ccgtccgtct tcgttaccca gcagcgtttc
     8941 agcgcttcgt tcgggatgcg gcatcatccc caacacattc ccgtcagcgt tcatgatccc
     9001 ggcgatattg tcgagcgagc cgttgggatt tgcctcgggc gtgatcttgc cctctgccgt
     9061 acaatatcga aagatcactt gcgcattggc cttaatgccg ccaagcgtca ccggatcggt
     9121 atagtagttg ccgtctgcat gagcgatggg aatcttcagc acctgaccgg actcgcagcg
     9181 attggtgaaa cgagtggcgg cgttctccac cttcacatac acatctttgc agatgaaatt
     9241 cagggaggta ttcctcaaca tcacaccggg taacagaccg gcttcgagaa gaatttggaa
     9301 gccgttgcaa atgccgatga ccagcccacc gtctcgcgcg aatgttttaa ccgcgcccat
     9361 gacgggggag aatctggcga tcgcgcccgt cctgagatag tccccatagg aaaaccctcc
     9421 cggcaagacg acggcatcca accctgcggg caaggtgtcc ttgtgccaga tcatcttcac
     9481 agactggcca agcacgtctt taaagatgta ctcgcaatcg tgatcgcaat tgctgccggg
     9541 aaacaccacc acgccgatgt tcatgtcact catcctcttg cggccagctg accggagccg
     9601 gtcgtctgat gcgctcgcga cggcagcccg tctgcatctt cattcggaac gagcctgagc
     9661 tcagcgttac cagccgccgc gtcactgcag ctcataccga tattcttcga tgaccgtatt
     9721 ggccagcagt ttctcacaca tctgcttgac ctgcgcatcg gccttggccg aatctgtttc
     9781 attcacatcc acttccatgt acttgcccac ccgcacattt gccgcattct tgaacccgag
     9841 cgagtccaag gcatgctcga tcgctttccc ctggggatct aatatcccct gcttcagcgt
     9901 gacatgaatt ttggctttca ttgtactttc cctacgcgct gcgtctgcgc ggttttgttc
     9961 acatacttgc gaatccagaa aatcaccccg atcgttccca gtcccgccat tgacaagaga
    10021 ataaacgccc agcgccaggg cgattcatcc agatgatacg ccatcagcgc aaccgtggca
    10081 gcccacacca aaatcgccag caccaacccg taatctaaaa aaaaccgcag aaacctattc
    10141 atcgtgtcgc gtcccgtttc tcgacatccc tcggggacga tatgcctggg actgttgcgt
    10201 ttgtcgaagg agggacttca tcgctccgta ttgcagaggg atggccgttc tcgtgagttc
    10261 cctactcaca gacccgcttc aagacttctt gataggcctc ttcgatcttg cccagatcct
    10321 tccgaaaccg atccttgtcc atcgattctt tcgtggcctg atcccagaac cggcaggtgt
    10381 cgggagagat ctcatcggcg agaatgagtc gattgtgaaa gacgccgaat tccagtttaa
    10441 aatctaccaa cagcatacgt cgctctttga agaagggtaa caagacggca ttgatcttgt
    10501 ggcccaattc acgcatctct cgcagtacgg ccggtgtggc aacgttcaag acgcgcaaat
    10561 gatcgtcgtt caccaacgga tcgcccagcg cgtcgtcctt gtaatagaac tcgatgatgg
    10621 ccggttcgat ggctgcgcct tccttgagcc ccaaacgctt ggcgagactc cccgccacta
    10681 cgtttcggac cacgacttcc accgggacga tggtcacttt cttggtgagc atctcgcggt
    10741 cgctgacgcg ctcaatgaaa tgggtctgaa tcccctgctg ctcgagcagt ttgaacagcc
    10801 gctccgacac cttattgttg accacccctt tatcgacgat ggtcccgcgc ttttgcgcat
    10861 taaacgcggt ggcgtcgtcc ttgaaatact gcaacacctg atccggccgt tcggtcgtga
    10921 aaattttctt cgccttgccc tcgtagagca tcgtgcctgc cggttcgcca gtcatcctca
    10981 atcctccaac taccgtgcga acaattccag aatatcgtcc agcggtctga tcgttcccaa
    11041 caatgtggga tgcccaaacc gccggtcatg gcgatattcc tttacctgtt caagcgcgcg
    11101 gatcaaaccg tggaactctt ccagcttatc cgtctcgaaa taggtgatga agtcaaagtc
    11161 gtccaagccg ctggaatgat acagtttccg tttcacggta ttgatatagg gcagcgccgc
    11221 ttgcgtatgt tcctgcatga gcctcgcgcg gctatcctga tccagcgccc accaatcggc
    11281 atcctttcgg atgggaataa cgatcgcata ggacttcgtc cctgtctccg gcgccggcga
    11341 ctgcaacgcc tgcttcatgg attcgggaaa cccggctgcg taggcagccg gcttggtcat
    11401 cccatgcaaa tacgtcgtcg ccaccagatg gcgtcccaaa cgggtgccca acaacgaggt
    11461 gagaaactgt tgcgtatcag acaaggcgtg ggcatggatg cggaacatca agtcagcatg
    11521 gtccgaaagc ccacggagca aataggactc gatgacgatc ttctgggaga actgctcgat
    11581 gagccctttc acctcagaca cggcgatcac gcgggcttcg ccattgagat cccaccaatc
    11641 gtgatccatc tggaacgcag cgaaggtgcc gtagacgccg ggctgtgtca gcagggcctc
    11701 ccgctcggtc gcaccgaccg gcgacacggc cgccccgaac accagggcca ccatcagcac
    11761 gatccgcatt attaatgcag ccatcatgga cgcctcttgc ggcgtcgcgc ggctgggatc
    11821 gcacgggcgc cgaaaacccg gcgaaacacc tgatcgagat gccgcagata atacatgggg
    11881 tcgaaacaag cggtgatctc gctcggcttc aaatgagtcg tgatgaacgg atcccggttg
    11941 acgagttcct gaaatccgac cccgcctttc caggctgtca tggcattgcg ctggaccgcc
    12001 tcatacgact ccttgcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncag
    12121 acatattctt cgatttccgg cccctgatgg gcaaatgtcc ccatcgcgcc ggaaagtttg
    12181 ccgacggcaa tgtctttcct ggccgcctca agacgtgtcc ggtgccggca cgcctcttcg
    12241 taccagattg ccaacttgaa tccgaacgac accggctcgc catggatacc atgcgaacgg
    12301 ccgaccatca ccgtatggcg atgctccaac gcgcgctttt tcaaaacggc aatcaactca
    12361 tcaagatctt cgaggatgag atcgagcgcc tccgtcatct gtaccgccag ggacgtatcg
    12421 acaatgtccg atgaggtgag ccccatgtgg agatagcgat gctcgggccc gacggacgcc
    12481 atgagcgatt cgagaaacgc gatcacatcg tgcttcgtca ccttttcaat ttctgcgata
    12541 cgcgccacat cgatcttggc ctttttccgg atctttgcgc tcgtgccacg cgggatctgt
    12601 ccggcccgtt caaaggccgc gcaggcctgc agttcgactt ccaaccaaat ctcgtacttg
    12661 tgtgtgagat cccagatcgc gctcatgcga ggccgggtat atcgctcaat catcgccacc
    12721 tcatgaaacc aacaccgtga atcacaaaca gcttcaagtt ttcagcttcc atcgtcttct
    12781 catcgtctga cctgtacggt ccaggcgacc taatcgctcc cgacaaccgg taattccggg
    12841 aacgcgcgtt tccgttccag ccgactatcc gatcccagca ccttgtcgag aattccattt
    12901 acaaacttgg aggcttcttc atcgccgaaa cttttcgcca gctcgatcgc ttcattcagc
    12961 gtcaccttcg ccggaacctc agggacaaaa agcagttcga agcagcccag gcgaagaata
    13021 ttccgatcga taatgggcat ccggccgacc ttccaattgg tggcatgggt gccgatcaac
    13081 gcatccagtt ctttcttatg ctctctgacc ccggccacga ggcgctcggc gaagacgcgg
    13141 agctcatcat tcagcggata ttgcgtccaa aaatcgtcca gccatggacc ggcctggcca
    13201 tgaatgtcgt attgaaagag aatctgtacc gcccgttcac gggcttggtg tcgagaggcc
    13261 ataagacaac gccggtcagc gtttcgccct cgcggccgtt cgtccggctc ttcgtggagc
    13321 ggggcccggc ggcacgtcct cgctctcttc ttttttgagt tgccgcatca cgttgaccat
    13381 ttcaatggcc gttttggcgg catcggcacc acgattgagc ttcgccggat ccgcacgttc
    13441 aatcgcctgc gccaccgtat cggtcgtgag caccccaaaa atcaccggga gattcgattc
    13501 catcgaggcc tgcaggatac cccggcttac ttcggtactg atgtattcaa aatgaggtgt
    13561 gtcgcctcga atgacggcgc ccaggcagat gactgcgtcg aatcgaccag accgggcgag
    13621 ttggcgagcc accagaggaa tttcgaacgc accaggcacc cgcacgacct caatcgcctc
    13681 gtccgacgct ccggccttgg tcagcgcctt cacgcattca gtgagcaggc ggctggtgac
    13741 gaacttattg aatttgctcg ccacgatccc gacggtcaga cccgtcgcgt cttgggagcc
    13801 cttgcgaagc ttcatgccgt gcgataccga ttcagacgtt tcatggattg cgcgtgcgcc
    13861 gaagaaagag ggcgatcata cctggaaacg ccatcaaatg gctacacgtt tcaggcgcat
    13921 ctcttcctgg cgaaggacgt acgaaataaa gcgcggcaga cgcgtcagac ttttttcaac
    13981 aggtggccga gtttggcctt tttggtgcgg agatattttt cattatcagc gcgtggagca
    14041 atttcaatgg gcacacgttc gaccaccttc aacccgtacc cttcaatccc gacaatcttc
    14101 cgcggattgt tggtgatcag gcggatctta tggagcccga gctggacgag gatctgcgcg
    14161 ccgaccccat agtcccgcaa atccgccttg aagccgagtt gaagattggc ctcgaccgta
    14221 tcactgccgg aatcctgaag gccgtaggct ctgatcttat tcagcaggcc gattccgcgc
    14281 ccctcctggt tgagatagag caacactccc cggccgtcct tctggatgat ctccatggcg
    14341 cgatggagtt gctcgcggca atcacagcgc aacgatccca gcacatccgc cgtcaggcat
    14401 cccgaatgga cccggacaag catgggcgtg tcggcatcga cctgtccctt caccaaggcg
    14461 atgtgagtcg ccccgtcgat atcattctcg aacgcaatcg cctcaaaatc accgaacgtt
    14521 gtgggcaaga acgcactcgc cgcccgccgc acgaaggtct ctcgatgcat ccggtattcg
    14581 atcaaggctt tgatcgtcac catttttaat ttgtggcgtt tggcgaactt ggtcagctcg
    14641 gccacgcgcg ccatcgtccc gtcctcattc ataatttcgc aaatgactcc ggcgggatag
    14701 agcccggcca gtcgagccag atccaccgac ccttcggtct gccccgcacg gcggagcaca
    14761 cccccgactt gcgccttcag aggaaaaata tggccgggac gcgccagatc agccggagaa
    14821 caacggggat cgatggccac gtggatggtc gtcgcgcggt cggctgccga aatgcctgtc
    14881 gtgatacctt tcctggcatc gatcgacacc gtgaaggcgg tgccaaaggt ggccgtattc
    14941 tccgcagcct gcggggccaa ctgcagttcc tcaacccgtt ccggcgtcag ggccaagcag
    15001 atcaacccac gcgcatgctt ggccatgaaa ttgaccgcct ggggcgtgac cttgctggcc
    15061 gccatcacaa gatcgccttc gttctcgcga tcttcatcat cgacgaggat gatgaacttc
    15121 cccttcttga tgtcccggat ggcttcttcg atcgaattaa acggtgtcgc catagtccct
    15181 caattgtacc gaccggcctg cgtgggtgcc cagctaactc ttttcaaacc atgcctgtca
    15241 acgggaaaaa ccttccgcat tcgtggctga tgcgaccgcc cggggtcgcc accgtaaggc
    15301 ggaacccacc gtcatggcgg cggcggcact caccagcgcc aatccgagaa aacccggcac
    15361 gaacgtcccc atggcttccc gcaataatcc gaacccgatc gggacaaaaa atccaccgat
    15421 cccccctgcg gcccccacca ggcctgatgc cagcccgata tccttgggaa accactcgga
    15481 gacgatttga aagatcacgc cattgccgaa ccccataacg gcaatcgtca ccatcagcag
    15541 gctcgccagc cagggcaata aaggcaacga agcggcgaat gccgtgacgg cagcgagcac
    15601 cataaacaca cccgccaacg cagggaggcc gccccagcga tccgccacat acccgcccac
    15661 cgggcgaatc aggctgccgg tcaatccgca gagcgcggca atggccccgc cgacaatcgg
    15721 atcgctgccg tactgatcgt gcagcagcac cgggagaaaa cttgtcaggc cgacaaatcc
    15781 gccgaacgta atggcataga cgaggcagag ccaatagaca gagggcactt gcaccattcc
    15841 ccaggcctgc tgccaccagg catcttctgc gacggagata gtatgcggtt cttcccgcac
    15901 gacacgagcg aacagaccga tggccagcag aattggcagc gccatgatcc cgcaggcccc
    15961 gtgccatccc aactgcccct cccattgcgg cgccagcaga aggaccaaca ccgtgccaat
    16021 attgccagac gcgaccaatc ccaacaccag gccttgatgg gcggccgggt acgctcgact
    16081 ggccaccggc atggccaccg caaaacttgc tcctccggca cccagaagca atgcgatggc
    16141 gagcagctct acgtactgtg tcccactgag cgccgcccag aggactgcga tcagttgcaa
    16201 ccccaagacc cacagaccgg ttcgtttggc tccagaccag tcgcaggccc agccggtcac
    16261 aattcgcagc agcgcaccac tcagaagcgg aagcgccacc accacactct gttgcgtcgc
    16321 cgtcaggtgg agctccgcgc ccatcggcac tgccagcgcc ccgaacagca cccacaccat
    16381 aaaactcaca gtgagatgca accaggcacc tgccaaggtc ggccaatgcc cgctacgcag
    16441 agccgttccc aatgttctga catttcctaa cgacttcatc ttatgcggcc agggtccgag
    16501 ccttacgaat ctctttcatc atcacgcact atacgaggcg cgatttcaga gacgcaagca
    16561 aggccgatgc agatcagacg tactcattgt ttcccaacac tcgacatctg gtatagtcag
    16621 ctcctgtatg caaagggttc gactcgcatg agacagcgca agccaaagca taataccgat
    16681 tctgactccg attcagctgc ggagttggac gaagacgaca gccttgaacc cgaggccctg
    16741 gagccaacgg acgaggaggt gacggaagaa catgaggcgg ccgagcggca accatcgaag
    16801 gagtcgccgt ctgcaccatt gagcacttcc ctggtccccg tcacgaccct gcaacaatat
    16861 ctgacggaaa tccgccgcta cccctacctc agcaaggaag aagagttacg cctcttcgag
    16921 gagtatcagc ttcgcggaag ccgggatgcg gcgatgaaac tcattcttgc caatctgcgg
    16981 gtctccgtct ccatcgcttc cgaatacctg cacacgggcg ccgatcatat ggacctgatc
    17041 caggaaggaa acgtcggcct cctgcaggcc atcaagaaat tcgacccgac gaaaaacgtg
    17101 cgcttttatg cctacgcagc ctggtggtcc agggcgtata tcctccgcta cctcttgaac
    17161 acctatcgcc tgatcaaggt cggcaccacc caggatcaac gcaagctgtt ctacaatctg
    17221 aagaaagaaa aggccaagct cgaacgcgaa ggcttcgcgc cagacagcaa gctgctggcc
    17281 gaccggctga acgtccgtga acgcgatgtc atcgagatgg atcagcggct gggcagttgg
    17341 gaattgtcgc tggatcaacc catcggccag gaacatgacg ggacgttgat ggacgtgctg
    17401 cctgcgcatg aacagccggc agacgagcaa ctggcggaca cgcagctgaa agcactcttt
    17461 cggcgcaagc tggcggaatt taccaaaacg ctcgacgagc gagaggaaga cattctccgc
    17521 aaccgcattc tgagcgaaac tccactcacg ctggaggaca tgggcgcaaa gtacggcatc
    17581 accaaggaac ggaccaggca actggaggcc cgcatcatca aacgactgcg tgatttcatc
    17641 aaaaaggatg tgaaggactt tgaccgtctc cggatgtaag accggggcta gcatcagaat
    17701 cgtcctcacc attctgatgc ttacgaccct cgtcgaatcg gggtgtccgc gcccttccca
    17761 cgccgcctcc ctcatcaccc aggaaaatgc ccggcccggt caatccgact gggttctgac
    17821 cgatcccgcc gatcatgagg tcgagggtta tgcctcggcc accagcatcc agcgcggaga
    17881 accgcttcgc ctctacattc acagcaccga tccccatgtg acggtcgaca tctaccgtat
    17941 gggatggtat ggcgggacag gagctcgatt ggtgcgggga ggcatcacgc tgccaggcgc
    18001 tcgacaaccc atgccgacac cggatcccgt gaccggtctc atcgaatgtc gatggcaagt
    18061 ctcatacacc ctgacgacag agacacatga gaagaacgac tggctcagcg gcgtgtacct
    18121 cgcgaaactc accggctcgg ccagcggcaa acagagctac atcattttcg tggtcaggga
    18181 agacgatcgg ccctcggagt ttctctttca atccagcgtc accacctttc aggcctacaa
    18241 caattggggc gggaaatcga cctatccgtc caacagccgg gatgagcaat gggcacgaaa
    18301 ggtctctttt aaccggccct atgccaggag ccaacatccc cagggcggaa gcgggattgg
    18361 ggcgggagaa ttcctgaccg cgatgtcgat tcatccaacc cgcgcgctct cgaccgcggg
    18421 ctgggagtac aacatggtgc gttggctaga acgtcacgga tacgacgtga cctatagcac
    18481 cgacatcgac acccataccc gggcagaatt ctggaagggc cataagacct ggctgtccgt
    18541 ggggcatgac gaatactggt cggctgagat gcgacgccat gtcgaggagg ctcgagacca
    18601 cggagtcggc ctgggttttt tttctgccaa tacctgctac tggcagatca gactggagcc
    18661 cagcctcctt accggcgagc cgaatagaac catggtgagc tataaagaag tggcatggaa
    18721 cgaggatccc ctggccctgg atggcgatcc atcaaacgac cagttgatca ccgtccaatg
    18781 gcgtgaatcg ccggttcaac gaccggaaca ggcattgctg ggcgtcatgt atgggaccgt
    18841 cccggtcgat ggagatattc tcatcacgca gcccgagcat ccgctgttca acggtgtgga
    18901 tctgccgccc gaccatcgcc ttcccggcct cctgggatac gaaatcgacc aggtattcgc
    18961 caacggacca gccggtttaa ccgtcctggc tcattcctcc tatccgaggg atggggaggc
    19021 agtttatggt gacatgaccc tgtatcaggc ccagagcggc gctttcatat tcgctgccgg
    19081 gacgattcaa tggagctggg gcctcgacga ctataatgcc ccgacattgc ggaccgcccg
    19141 atccagtcag gcggcgcaac gaattaccgg caacatattg agattgctgg cctcacccgc
    19201 ctccagcccg gctcattagc gacaccgggc ggcttcactg cgacggccga cgttcggttc
    19261 cggtcaggcc ctacgatgtg cttcagaaga cggctgcgag gagatgggct acgaaattcg
    19321 tagaatcaac aacttgcgcg gcactttccc attgcatctc gcggaagaat aattttatag
    19381 tccatccatt gacttccctc gtcatagcgc tatctatgac tcgagttttc tgcaggttgc
    19441 ccttgcagta acacgtcaga gctgaatttc cagctttcac acaactgtac attagggcct
    19501 cgacgctcat taatctagaa ggaggattgg gtatggctac ggaatcgaag gaaaagaagt
    19561 ccaccgctgc gaagaatttc cagccgctcg gtgaccgcct gttcgtcacc tacaccgagg
    19621 agatggaacg gacatctggc ggaatttatg ttcctgactc cgcgaaagag aagccgcagc
    19681 ggggaatcgt gcaggctatc ggcaagaaag ttgagaacat caaggtcggc gaccaggtgt
    19741 tgttcgacaa gtattccggc agcaagctgc ggatcgaaga cgaagagtgc ctcatcctca
    19801 aggaagaaga catcctgggt atcttcaccc actaatctca cgcacccgac taacgattat
    19861 tctgacgatt aacggaggac gattatggca aagcaattgt tgtatagcga agcggcacgg
    19921 gcagccatcc tgagaggggt gaaccagctc gccgatgccg tgaaggcgac gctcggcccc
    19981 aagggccgga acgcgatttt ggacaagaag ttcggcgccc cgacgatcac caaggacggc
    20041 gtgacggtgg cgaaggaagt cgaactcaag aatccgtacg agaacatggg cgctcagttg
    20101 gttcgcgaag tcgccagcaa gaccagcgac acggccggtg acggaaccac caccgccacg
    20161 gtgttggccc aggcgatcta ccgggaaggc gtcaagaaca tcaccgcagg tgccaatccg
    20221 atggaaatcc agcgcggcat caacaaggcc gtggaagtcg tgatcggcga gttgaagaag
    20281 ctcagcaagc cctgccagaa caagaccgag atctcccagg tcggcaccat ctccgccaac
    20341 aacgacaaga ccatcggcga cctcatcgcg gaagcgatgg agaaggtcgg caaggatggc
    20401 gtcatcacgg tggaagaagc caagtcgatg accacctcgc tggacgtcgt cgagggtatg
    20461 cagttcgatc gcggctacat ctccccctac ttcgtgacca acgccgagcg gatggaagcc
    20521 gtcatggacg agccgctcat cctcatcaac gaaaagaaag tcagcagcat gaaggacctc
    20581 ctcccggtcc tcgagcaggt tgccaagctc ggcaagccgc tcatcatcat tgctgaagaa
    20641 gtcgaaggcg aagcgctcgc gaccctcgtg gtcaacaagc tccgcggcac cctcaacgtg
    20701 tcggccgtga aggcaccggg cttcggcgat cgccgcaagg cgatgctgga agacatcgcg
    20761 atcctcaccg gcggccaggt catctccgaa gacctcggct tgaagctcga gaacgtcaag
    20821 ctgacggatc tgggccgcgc caagcgcgtc acgatcgata aggataacac cacgatcgtc
    20881 gaaggccatg gcgacccgaa gaagatcgaa ggccgcgtga agcagatcaa ggcccagatc
    20941 gaggaaacca cctcggatta cgatcgcgag aagctccagg agcggttggc gaagatcgtc
    21001 ggcggtgtgg ccgtcatcaa cgtcggcgcc gcgaccgaga ccgaaatgaa ggaaaagaag
    21061 gcgcgcgtcg aagacgcgtt gcacgccacc aaggcggccg tcgaagaagg catcgttcct
    21121 ggcggaggca cggcctatct gcgctgcctc aagggcttgg attccctcaa ggacttgccc
    21181 ctggagcaga aagtcggcgt cgacatcgtg cgccgctcac tcgaggagcc ggttcgtcag
    21241 atcgcagcca acgcgggtgc cgaaggctcc gtggtggtcg gccgcgtccg tgaggacaag
    21301 aacccgaacg gcggctacaa cgccgccgcc gacgagtacg tggacatgat caagaccggc
    21361 atcatcgatc cgaccaaggt gtcgcgctgc gcattgcaga acgccgccag cgtcgccggt
    21421 ttgatgctga cgaccgaagt catgatcacg gaattgccgg aagagaagaa agagcctgcc
    21481 ggtggccatg ctggtcacaa ccacggcatg gaagggatgt actaaggttt ccgtttcttc
    21541 ttcgctgtcg aagaccggtc cggcggtggc cccgccgggc cggtctcttt ttcgagccga
    21601 gacgggt
//
LOCUS       DAOQDS010000060        21245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_936_length_21245_cov_21.325389, whole genome shotgun sequence.
ACCESSION   DAOQDS010000060 DAOQDS010000000
VERSION     DAOQDS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 21245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21245
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_936_length_21245_cov_21.325389"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..995
                     /locus_tag="PK589_14065"
     CDS             240..995
                     /locus_tag="PK589_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNJ20782.1"
                     /translation="MLEWLANPEIWIALGTLTALEIVLGIDNIIFLSVLVSRLPESQR
                     AVARRVGLGLAMIARLGLLFSISFVMGLTKPWVTVLGHGISGRDLILVGGGLFLMAKA
                     THEIHNSLEGVEEGHSPTAAASLGMVLVQIALLDIVFSLDSVITAVGLVEHVSIMAAA
                     IILAVVVMLMAAKAIGDFVETHPTIKILALSFLILVGVTLMVEGFDVHVPKGYIYFSM
                     AFSVTVEMLNIRMRKKRAVPVKLHSRYAEEPRQ"
     gene            complement(1023..1565)
                     /locus_tag="PK589_14070"
     CDS             complement(1023..1565)
                     /locus_tag="PK589_14070"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNJ20783.1"
                     /translation="MNGLLTRLLACSASLILVTACSTLPSKYIEKADPGVTLTSLAAA
                     PETYQGKTVILGGVVVDQQQQGQRLWLHLKNRPLDKDYRPHRPTINEGPEAGYYWVVV
                     PDASLLPPKWKQWARVTVVGRVMDQRETRPTTVPTSEPVLSLVFMRGWAMGNAQGKGA
                     WEESVDANYLLSVPEGLHGE"
     gene            complement(1606..2415)
                     /locus_tag="PK589_14075"
     CDS             complement(1606..2415)
                     /locus_tag="PK589_14075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ20784.1"
                     /translation="MSRSSASPSTRWPLPAQDDWSTPFAELLLAQLDLHSGLTILDIA
                     CGHGIPAFYLAEHVGPTGSVLGIDMSRGQVANARTVQRGELPWLRFECVDMRALPPSF
                     PTFQRITGNLSVMFLRPHRFEAMRGLIAHLEPGGQIVLTFPSLGTFDSMWQRIDREMS
                     RHGLTMERERLAAHVAERPSAAEARGWLEQLKMERIDVIEQPLEVPSGAGQRFLQHPL
                     LRGGFLDDAYECFDDARLADTVMTSVAADLESMMPLVARRCALSGWKPRQA"
     gene            complement(2507..3250)
                     /locus_tag="PK589_14080"
     CDS             complement(2507..3250)
                     /locus_tag="PK589_14080"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNJ20785.1"
                     /translation="MAPGTERPCFSCDDQRLVRMVPLEKGTHQKLLLRHPLVLQQQEW
                     EALLRMVKVRSIHRPLLGATYQGDTEPLFSEEEVRYLGDSLQRAFQQATPQEHVVFAL
                     ARPSQTGLEQITSGAWFVEDGLIHLRVANCRIAVTMPSIRKQIWKDPLFAQAGAFYEF
                     VPGGGQAIARATVDGGNPFRSDPDELLIEYKVASSANPAPVSSEPSAQPVPPRSLEEQ
                     LSLLKRLLEQGLITEEDYRTKKQQLLDRL"
     gene            complement(3362..4831)
                     /locus_tag="PK589_14085"
     CDS             complement(3362..4831)
                     /locus_tag="PK589_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20786.1"
                     /translation="MKMSTLRARAVIVAILILLGLISYELSGLVQKAEAIPAFGRKYD
                     FKCNVCHVPGFPKLNDFGNLFRDRGYQLGSDADSPVYEGIGMGFWPVSIRATVGYQAA
                     QLRTEGSGVTTGGFGFTGLDLLSFGTLHRDIAFGVVYTPGLGSAGFGTGASDGDLESA
                     FVRLMRLERYFGVKSDPGNYLLNLRVGKFELDLPFSEKRSPTLNTPFVMYHYTPGVPY
                     TATIGGTSTSSYLNPNSFLLGDNQPGGELSGIMKTPSTEGYLRYSLTALATNSFSGPL
                     GGCPPNTPCGTGGRNVNFYGHITQSFGGYGIVTGNRVGIFGAYGQAPTMVNATCPTCQ
                     GVAGSGKPFTRIGVDVSLTFNGEWNIFGAIMHGHDSAQLFASQGIPTPQNASWNGAFV
                     ELDYAPALFFDMPDWFFAYRYDVIRNDRHGDGNTDVFKGNYNDVDSHTVLARYFIHHS
                     TRTDLALHAEYNSYRTQAVGPTGGDLRGQTMLVGLDFAY"
     gene            complement(4871..5734)
                     /locus_tag="PK589_14090"
     CDS             complement(4871..5734)
                     /locus_tag="PK589_14090"
                     /inference="COORDINATES: protein motif:HMM:NF026037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ20787.1"
                     /translation="MSPRRHRGFRLVLLSCLAWLLTAISGYAEYEIIDVSDGGTVKGQ
                     AVWKGSIPKLPPLKVFADLDSCGTHVPSPALRIDPATNGIHEVLVYLERVERGKSAAT
                     AYPLRMGKSASHPTGRACQFERHMIPFVRTAEVALINFEPILHNPHLFSEKQSLFNIA
                     MPTANREIATTLGRARGVGLRLQCDVHVHMNAWAAALEHPYFAVTDEQGKFEIAGIPP
                     GSYTLVAWHPGFNIVKFSASRPVYDEPHVIRQPVEVSSKAQVESRFEFPVRPVEVEWN
                     IAGGGDELPPE"
     gene            complement(5990..7003)
                     /locus_tag="PK589_14095"
     CDS             complement(5990..7003)
                     /locus_tag="PK589_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNJ20788.1"
                     /translation="MAGRPLVPDHILQIGFGFWSSKTLLSAVELGLFTLLAKRQLDGK
                     ALAARLKLHPRSARDFFDALVALKLLKRNGTRYANTPETALFLDRAKPTYVGGMLEMC
                     NARLYGFWGSLTEGLRTGQPQNEVKTGEDFFGTLYADPQRLEGFLKAMTGLSKGTARS
                     IAAKFPWKRYHSFADIGCAQGGVAVEIALAHQHLNGQGMDLPVVRPVFESYIRSKKLQ
                     KRMTFHEGDFFKDSLPKVDVLIMGHILHDWDLKEKMMLLRKAYAALPPGGALIVHEAL
                     IDDARKTNAFGLLMSLNMLIETPGGFDFTGADCRQWMKKVGFTRTKVEPLAGPDSMVI
                     GFK"
     gene            7274..8017
                     /locus_tag="PK589_14100"
     CDS             7274..8017
                     /locus_tag="PK589_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20789.1"
                     /translation="MATYLHVKAALTALVLFSIMTATAGAEQWNGPQTPTGTIWRPGN
                     IALGFDPAGHSGTPPALEIRRPLSGTEEVLLSLRSTFKEATATVLEVDTKRVYMGGAA
                     TRTSLVPDGVFDLAVGRGIAVGVNSVTERLPTDYRLVVGGKVLAEEVRIKLIKDWADY
                     VFHPDYRLAALPEVATYIQSHQHLPDMPSAAEVAATGISLGDMQAKLLRKIEELTLHA
                     IEQHQTIAALQARLQQLEEDRSSQQLGSK"
     gene            8055..8972
                     /locus_tag="PK589_14105"
     CDS             8055..8972
                     /locus_tag="PK589_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20790.1"
                     /translation="MTQFTALTTALLLATLTLPAEAGTTSLTLNQVIKEGTARYYDHP
                     QELRLADGFWAQAGSDMTATIHLQQGYYLDKTPLDTKEKQYTSTDGRTYLVAGCVPKH
                     LPISGPVGSVWVACNDLDYLKDKVVESRFVSCLDVFTNGVVSGVSIKEVNNRTWESFR
                     FSCRDMGPDGSMVGAAQKSDFLFNYEREGKLYETTTASGHLTFGILEVANGLTGTKSL
                     LQIALEHTAAAALQAAGVNGRHVDDFTLSDKVPDAFGLGGMLRVDHWKCPPGMVITGM
                     AIGHNPDKKGNDTKPVYILAECRKLLYTP"
     gene            9137..9802
                     /locus_tag="PK589_14110"
     CDS             9137..9802
                     /locus_tag="PK589_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20791.1"
                     /translation="MATMLQKALQGLLLVLLVGCSTTYEPHLVLPPAEFNRSIDAAVE
                     VHPFVAADDLLYGRSNYGVVADDYANKPPSKLTQSITQQVTAELAAHRVFRRVSTYDP
                     APDLILTGRIERFFEHDRRKLWTFIPYYSDKLASLFRVNSFKTNGEIHLTLTLLKPTG
                     EVVGSYVGHASFDEDYTPNGEVRPGDRLNRALGQALAQIRDEMFADSTLTKSRTPHPL
                     ALQ"
     gene            complement(9853..10308)
                     /locus_tag="PK589_14115"
     CDS             complement(9853..10308)
                     /locus_tag="PK589_14115"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNJ20792.1"
                     /translation="MLDVPVLRTNEDFEKGQAPDSIRRAEESMGWAGHLVIVCPLWLG
                     SMPALLKAFFEQVFRSGVAFQYQPDATRAKKLFAGKSARVVVTMGMPAWVYRWWYHVH
                     SLKGLQRNIIQCCGIKPTRATLIGNIESMNEQRRASWLDEMRGLGERGQ"
     gene            11006..11443
                     /locus_tag="PK589_14120"
     CDS             11006..11443
                     /locus_tag="PK589_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ20793.1"
                     /translation="MRATEYFARGCDPNTLVVRQIMEDAVVTIHPTSSAMAVAEILSE
                     HSFGSVPVVDKDGTLRGLVTEFDLLKAVEQERDLRSVLVSDIMTKDVVTTTEETPIMS
                     LIHLLQERHLIRVPVVKGQKLVGMVARRDVVFAYVKARAIYWP"
     gene            complement(11498..12175)
                     /locus_tag="PK589_14125"
     CDS             complement(11498..12175)
                     /locus_tag="PK589_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HNJ20794.1"
                     /translation="MKTVQRGGNAEAGHSRFAAGLLLGYGIIWIWLAINPVNRHDWVL
                     ENLLAVGLVAILVLTYRRFTFSLTSYSLIAVFMVLHAIGAHYTYAEVPFGFWLKDIAG
                     LTRNPFDRLVHFSYGLLLVYPLREVLVRLAGVQGSWSYVMPVSSILAQSAFFEVIEAI
                     VAMIVSPELGSMYLGTQGDEWDAQKDMAAAFGGAIVTMVMTAALSAPRNSQVQQPSHR
                     SAPSVAH"
     gene            complement(12190..12828)
                     /locus_tag="PK589_14130"
     CDS             complement(12190..12828)
                     /locus_tag="PK589_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HNJ20795.1"
                     /translation="MSRNKLISIGLLLWYLIVSIWMAQAPVDPQFWLLASILPALFVA
                     LLVVTHRMLPLSHTSYALITAFLTLHTIGVHYTYAEVPVGLWMDKVLHLGRNHFDRIV
                     HFSFGFLLAYPMEELFRLSANIRGWVVYYLPVMTVLGLSGLWEIIESWVARAVHPELG
                     VTYLGSQGDIWDAQKDMAAALYGALISMTVLLVLRALRGAETALESEAAFSE"
     assembly_gap    12905..13004
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13269..13997
                     /locus_tag="PK589_14135"
     CDS             13269..13997
                     /locus_tag="PK589_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ20796.1"
                     /translation="MPMPLLDIRSLSVVVDGQVILDRLDLAVQGREIHALVGANGSGK
                     TTLAYVLMGCEGYSPSAGHVLFNGMDLLRLKMHERARLGLTLAWQEPARFEGVTVREY
                     LSLGKPECDPEPALKQVGLDPDRYLTRRMDKALSGGERHRIELASVLSMKPKLAILDE
                     PVAGIDMLSIHHIIEIIRALKASGGSVLLITHQEEVVLIADRASQLCGGRIIFSGSPS
                     EAVDHFRGRSCVRCDGEVCGYVRP"
     gene            13984..14919
                     /locus_tag="PK589_14140"
     CDS             13984..14919
                     /locus_tag="PK589_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNJ20797.1"
                     /translation="MSDLEELMRVLPRVGAEPSILDDTGIAHVVAHGHHILSHRTVPG
                     LRVDIQETPEAIIGKIVVEAGVTIAQPVHLCFGLAHPTGTQRITMDMQVNEGAQVRVL
                     SHCLFPFVQDGQHRMQAVMTINPGASLTYTEGHYHGPYGGMQVLPHATIKIGKGARYF
                     SDFSLLSGSVGNLDINYLVEVEEDGLAELSAKIFAHQRDRLTLKEAVMLRGERSRSLI
                     KTRVVLEDDATAEITGITEAYAKGTRGHVDCMEVVQGRAHASAIPIVKVFHPEAKVTH
                     EAAIGSVDKKELETLMARGLNPEQAVELIVSGMLR"
     gene            complement(15309..16352)
                     /locus_tag="PK589_14145"
     CDS             complement(15309..16352)
                     /locus_tag="PK589_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNJ20798.1"
                     /translation="MRLVVTIDVEEDQWGIVPHGQATAQNVYRLPRLQALLNEFGIIP
                     TYLVTYPVMQDSYAVGILREIVEAGECEIGAHCHPWNTPPFKEPLSKRNSMLCNLPAT
                     LQYEKLQRLHEAIQYNVDRSPRAFRSGRWGFDTEVARNLLRLGYRIDTSVTPYTSWVQ
                     TAGPDFSCCSPQPYGFAPNVHTGRSSNEVLAEIPATIGYLHGEFQACATLADRLNRAP
                     FGRFRVGGLLSRLHLLRKVWLSPEMETPARMMQLVRQMRHQQYEWLNLVFHSSALLEG
                     CGPFVRSRADEQAFLKRLRTFLCAAVKEGILCVSLSEAARHFIPASGEPAATPDSSSL
                     MAKCPAFSSQPFA"
     gene            complement(16397..17521)
                     /locus_tag="PK589_14150"
     CDS             complement(16397..17521)
                     /locus_tag="PK589_14150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ20799.1"
                     /translation="MKQRRRLTIQVVTTPEEFKGLAGAWETLLDRMPGHSMFLTWEWL
                     YYWTLHYLGDSQLRILLAFDEHETLVGIAPLYLRRTKKHAVFAVRELRLLGSEAVCSS
                     YLDLIVQEQHKLAMLQSLYHYLFGDARDEWDLLTLSDLSAESSTLDLWNGLVAKAGKV
                     GDVSTTSCCPVIRLPADLPTYRASLGRNRRYTIQRKTRCLQQAGRMQFTRATSPADVE
                     IAFESVITLHQRRWSSRPGGGVFANEQSRRFHRDIVRVLSERGRVRIDLLLLDDRPIA
                     GVYGFLYQGVYYFYLPGFDPDILPKASPGLLLLHRVIEQALCDGAQMVDLLQGSQPYK
                     LEWSTGLRRLVTSRYYNRSASAAAVTLVDGAKQALKILLR"
     gene            complement(17518..19107)
                     /gene="xrtW"
                     /locus_tag="PK589_14155"
     CDS             complement(17518..19107)
                     /gene="xrtW"
                     /locus_tag="PK589_14155"
                     /inference="COORDINATES: protein motif:HMM:NF038142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase W"
                     /protein_id="HNJ20800.1"
                     /translation="MENSLVTPSATGTRPTSAGVHSRTPAYLLRNYSALWRITGLCLP
                     LLLLCAPVLHDLLDLWWNRYGFSHGFLVPLLSLYLVRLQWPTLKQLPVEPALGAGLLW
                     LVGAIALLLASEVAGILTTGSVALILVLAGLVLLLCGFAYLRALAFPLAYLIFMTPVL
                     DGLTEPLVWPFQLLTANMSVSMLRALGIPVILENSIDIILPTVTLEVARECSGAGLLI
                     AVLAIGLPLAALTLQTWWARVTLLLSSVAIAIMANWLRLTVMGVYAQAGGKDLHGPYH
                     ILQGLFVDWVAFAFLFAGAWLLGRLEHVPLRPSIHGEEKKCFSEPVSHRHASNRAWWL
                     ACVTLSVATLVLYGLDRGASGLKKELATFPGVVGDWVVDHRLNSASRVSLAAADDSLM
                     RTYRDSDGRRVNVYVAYTKSQRQGKELVGMETAPLHTGAIAAMLGRREDAIAVNRTVF
                     DERGRPVPSLFWYHINGSSYTDRVEAKLATIKQALLRGRTDGALVLVSAEPRSRENDD
                     PWKAQEEFVASALPLLREYLP"
     gene            complement(19149..>21245)
                     /locus_tag="PK589_14160"
     CDS             complement(19149..>21245)
                     /locus_tag="PK589_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ20801.1"
                     /translation="EQAAKHEVRGDGYVQQEQFREAMIEFKNAVRAAPDNPSLQWKLA
                     KAALKGGDPSTAYQALGRVVQLDPSHFEAKWSLGDLYLAGGKMDEVGKIAEELVSANP
                     QHPAGYLLRAAVALRAERNADAIAILKQAVTLDPKMVGPHLALANIHFAQQDIEQAAE
                     WYDRGVKADPASAGARIARGQFLFATGAPEEGRKEFHKAVELSSDQESIKLVLAERYA
                     MLGLREDAEHELSALIADRHSQRARKALAELKLTLGEVAEAKPLLQAILAADEHDPVA
                     LYLKGRVAFAERDLLQAASLFEAAIGRDTTLVGPHVYLGLIRSTQGRMDQAEEALREA
                     IRRDPSNQTAHLALARLYLTQEKPKEAEKEVSQTLRVNPASLEAAVLYGDVFVFGKNW
                     ARAEEVYGAISRQLPGQPIGYVKMAALRQLQARPAEAAELFAQALVYAPDDPVIMQDY
                     LVTLVGSKQSQQADRVLGAYLAKTSRDPNTWRLAGRVYVAQGKIDQAQKAFRKAVDLA
                     PDLALAHYELGQVQLLQNKLPAAVSAFQSALKKDETNSAIHTALGMVLARRGQVDAAN
                     GHYKRAFQLNPRDVVAANNLAANLSDQQELDDALGFARDALELAPSNPAIKDTLGWIY
                     FKQGRFDRAYPYLAEASAALPQHPVVRYHHAVALSKIGRQTEALSELKSALSLPGGFP
                     EAEQAARMVASNKMDE"
BASE COUNT         4689 a         6368 c         5851 g         4237 t
ORIGIN      
        1 tcctctctat cagagacctg gtgaagatgg ttgcgatccg ggaccggccg gatttcctca
       61 gacgaagctg attgactcgg ccgcgagggg tacgagcctc gcggccgagt ttcgattgga
      121 cccacgtttt gattgcgccc accacgtccg aacggtagac tctcctcata cgtgttgttc
      181 ccacaccttc tcgcatgacc gatcgagtca ctcgttccct gagatgaatg gagtcgctga
      241 tgttggaatg gcttgccaat ccggaaatct ggatcgccct cggaaccctc accgcgcttg
      301 agattgtgct ggggatcgat aacatcatct tcctgtcggt tctggtcagc cggctcccgg
      361 aatctcaacg cgccgtcgcc cgccgagtgg ggctgggtct ggccatgata gccaggcttg
      421 gattgctgtt ttcgatttcc tttgtgatgg gattgaccaa accctgggtc acggtcttgg
      481 gacacgggat ctcaggccgt gacctgatct tggtcggagg cggcctgttt ttgatggcca
      541 aggccacaca tgaaattcac aatagcttgg agggtgtcga agaaggccac tcgcccaccg
      601 ctgccgccag cctgggcatg gtattggtcc agatcgcgtt gctcgatatt gtgttctcgc
      661 tggattccgt cattacggcg gtcggcctgg tggaacatgt ctcgatcatg gcggcggcga
      721 tcattctcgc cgtggtcgtg atgttgatgg cagccaaagc catcggcgat tttgtcgaga
      781 cgcatccgac gatcaaaata ttggcgctct cgtttttgat tctggtgggc gtgaccttga
      841 tggtggaagg gttcgacgtg catgtgccca agggttacat ctatttctcc atggccttct
      901 cagtgacggt cgagatgctg aacattcgca tgcgcaaaaa gcgggctgtg ccggtcaagc
      961 tgcatagccg gtatgcggaa gaaccgcggc aatgaggggc cctcaggccg ttctctcccc
     1021 gcctattccc cgtgaagtcc ttcggggaca gacaggagat aattggcatc caccgattcc
     1081 tcccaggcgc cttttccctg ggcatttccc atggcccacc cgcgcataaa tacgaggctc
     1141 aacaccggct cactcgtcgg caccgttgtc ggcctcgtct ccctctgatc cattacacgc
     1201 cccacgaccg tcacccgcgc ccactgtttc catttcggag gcaggaggga ggcgtcaggg
     1261 acaaccaccc agtaataccc cgcttccggg ccttcgttga tggtcggacg atgaggcctg
     1321 taatctttat cgagtggccg gtttttcaga tgcagccaca gccgctgccc ctgctgttgc
     1381 tgatccacta ccaccccacc caggatgacc gtcttgcctt gatatgtttc cggcgcggcg
     1441 gcaagagagg tcaaggtgac tcccggatcg gccttctcaa tatacttgga tggcaaggta
     1501 ctacaggccg tcacgagtat gagactcgca gaacatgcca gtaatcgagt cagcagaccg
     1561 ttcacatcgt cctccgttcg tcgcaacctc gatgcaccga agcaatcagg cctgacgcgg
     1621 tttccagcca ctgagcgcgc accgcctggc caccagcggc atcatgctct caagatccgc
     1681 cgcgacgctg gtcatcactg tgtcggccaa ccgggcatca tcgaaacatt cataggcatc
     1741 gtcgagaaat ccaccgcgta acagcggatg ctggagaaac cgttgcccgg ccccgctcgg
     1801 cacttcaagc ggctgctcga tcacgtcgat ccgctccatc ttcagctgtt cgagccaacc
     1861 acgcgcctcc gctgcggacg gccgttccgc cacatgcgca gcgagccgtt cacgctccat
     1921 cgtcaaccca tgccggctca tttctcgatc aatccgttgc cacatggagt cgaaggtgcc
     1981 gagcgaggga aaggtcagca cgatttggcc gccgggctcg agatgtgcaa tcagtcccct
     2041 catcgcctcg aaccggtgcg gccggaggaa catgacggac aaattgccgg tgatccgttg
     2101 gaaagtggga aaggatgggg gcaaggcccg catgtcgaca cattcgaagc ggagccaggg
     2161 caactcacct ctctgcactg tcctggcatt ggccacctgc ccgcgactca tatcgatccc
     2221 gaggacggag ccggtcggcc ccacgtgctc ggccagataa aaagccggaa tgccgtggcc
     2281 gcaggcgata tccaggatgg tgagaccgga atgcagatcg agttgcgcca agagcagttc
     2341 cgcaaagggt gtggaccagt catcctgcgc aggaaggggc cagcgcgtag agggggatgc
     2401 ggaggacctg ctcatgacat tcctttcgcc gggggtgatg gctctcagcc gcatcttacc
     2461 cgaagggtaa aggacaagtc agaaatctct tcggtcacct tcacgctcac aaccggtcaa
     2521 gcagctgctg ctttttcgta cgatagtcct cctccgtaat caaaccctgc tccagaaggc
     2581 gtttgagcaa gctgagttgc tcctccagcg agcgtggcgg aaccggttga gcagagggct
     2641 cagaagagac cggagccgga ttcgcagagc tcgcgacctt gtactcgatc agcagttcgt
     2701 cggggtcgga acggaacgga ttcccgccgt ccacggtcgc tcgcgcgatc gcctgccctc
     2761 cacctggcac gaattcataa aacgccccgg cctgcgcaaa caagggatct ttccagatct
     2821 gtttcctgat cgacggcatg gtcaccgcga tccgacagtt cgccacacgg agatggatga
     2881 ggccatcttc gacaaaccag gctcccgagg tgatctgttc caatcccgtc tgcgacggcc
     2941 tggcgagcgc aaacaccacg tgctcctgcg gggtcgcctg ctgaaaggct cgctgcagtg
     3001 aatcgcccag ataccggact tcttcttcgg agaacagcgg ttccgtgtct ccctgatagg
     3061 ttgcgccgag cagaggccgg tgaatgcttc gcaccttcac cattcgcagc agggcctccc
     3121 actcctgctg ctgcagcacc aacggatgac ggagcagcag cttctgatgg gttcctttct
     3181 cgagcggaac catcctgacg agccgctggt cgtcgcagga gaagcaaggc cgctcagtgc
     3241 ccggagccat gcccgaacaa cctgagacgg cgagacacga aagccatagc acagcgacag
     3301 ttgccccgcg acgaaacgga tggttcacag aactataacc tttcttctgg gcagacgatt
     3361 ctcaatacgc aaaatccaat cccaccagca tggtttgccc tctcaaatca cctccggtcg
     3421 ggccgacggc ttgcgtgcga taactattat attccgcgtg caaggctaaa tcggttcgcg
     3481 tggagtgatg aatgaaatac cgggccagca cggtatggga atccacatcg ttgtagttgc
     3541 ccttgaagac atccgtgttg ccgtcgccat gccggtcgtt tcggatcaca tcatagcgat
     3601 acgcaaaaaa ccagtccggc atgtcgaaga acagggccgg cgcatagtcc aattcgacaa
     3661 acgcgccgtt ccacgaggcg ttctgcgggg tcggaatccc ttgcgaggcg aacaattggg
     3721 cgctgtcatg tccatgcatg atcgccccga agatgttcca ttctccgttg aaggtcaggc
     3781 tcacatcgac cccgattctc gtgaacggct ttccgcttcc cgccacgccc tgacaggtcg
     3841 ggcaggtcgc attgaccatc gtgggggcct ggccgtaggc cccgaagatc cccactcgat
     3901 tcccggtcac gatgccgtaa ccgccgaacg attgcgtgat gtgaccatag aaattcacgt
     3961 ttctcccgcc cgtcccgcat ggggtattgg gagggcatcc cccgagcggc cccgaaaacg
     4021 aattcgtcgc cagggcggtc aacgaatagc gcagatatcc ttcggtagac ggcgttttca
     4081 tgatgcctga caattctccg ccgggctggt tgtctcccaa caggaacgag ttgggattca
     4141 aataggaact ggtcgaggtg ccgccgatcg tcgcggtgta cggcacaccc ggtgtgtagt
     4201 ggtacatgac gaacggcgta ttcaacgtcg gactgcgttt ctcgctgaac ggaagatcga
     4261 gctcaaactt tccgactcgc aaattgagca agtaattgcc gggatcggat ttcaccccga
     4321 aataccgttc cagacgcatg aggcgcacga acgcgctttc aagatctcca tccgacgcgc
     4381 cggtgccgaa ccccgcgctc ccgagcccag gcgtatacac cacaccgaac gcaatatcgc
     4441 ggtgcagcgt gccgaaactc aagaggtcca ggccggtgaa accaaaccct ccggttgtca
     4501 caccactgcc ttccgtccgc aactgcgcgg cctgatagcc gacggtcgcc cgaatggaaa
     4561 ccggccagaa acccatgccg atcccctcgt agaccgggga gtccgcgtcc gatcccaatt
     4621 gatatccgcg atccctgaag agattgccga agtcgttgag cttcgggaat ccgggcacgt
     4681 ggcacacgtt acacttaaaa tcatacttgc ggccaaaggc cggaatcgcc tcggctttct
     4741 ggaccagccc gctcaattcg taactgatca gccccaacag gatgagtatg gcaacaatca
     4801 ccgccctggc acgtagtgta gacatcttca tcggatcagc ctccccttta ctaccggctg
     4861 ggttgattgc ctattccggc ggaagttcat cgccgccgcc ggctatgttc cattcgactt
     4921 cgaccggccg gacgggaaac tcaaagcgag attcgacctg ggctttcgaa gacacctcta
     4981 ccggttgccg gatcacatga ggctcgtcat acacaggcct ggaagcactg aacttgacga
     5041 tattgaagcc ggggtgccag gcgaccagcg tgtaggagcc gggaggaatg cctgcgatct
     5101 cgaatttccc ctgttcatcg gtcacggcaa aatagggatg ctcgagggct gccgcccacg
     5161 cattcatatg cacatgcaca tcacactgca gccgcaagcc gaccccccgt gcccggccca
     5221 gcgtggtcgc gatttcccgg ttcgccgtgg gcatcgcaat gttgaagagg ctttgtttct
     5281 cgctgaagag atgtggattg tggaggatcg gttcgaaatt gatgagcgcc acttccgccg
     5341 tgcggacgaa gggaatcata tgtcgttcga actggcaggc ccgccccgtg ggatgcgagg
     5401 cgcttttccc catgcgcaac ggataggccg tggctgcgga cttcccccgt tctacccgct
     5461 caagatagac caggacttcg tgaatgccgt tcgtggccgg atcgattcgc aaagccggtg
     5521 acggcacgtg cgtgccgcag gagtccagat ccgcaaacac tttgagcggc ggcaacttgg
     5581 gaatactgcc cttccagacc gcctgacctt tgactgtgcc gccatcggag acgtcgatga
     5641 tttcatattc cgcatatccg ctgattgcgg tgagaagcca ggcgaggcag gagaggagca
     5701 caaggcggaa accgcgatgt cgtcgtggag acatgaaaga cctcatcgac ataccgagag
     5761 ccggatggcc cagggcggtg aaaataaaaa agcccgaatc tccacaggag attcgggctc
     5821 gagagctgat cgctcgttgg cctcacttat gtgcgcacgg tggggagcct aatcaaccct
     5881 ccttcggcct gtcaagtatg cgatgccgca tgtaacacag gccatgcgtt ccgctcactc
     5941 actccgcgag acctcgcttc atcaggcaat ccctcccaga gacgccgatc tatttgaatc
     6001 cgatgaccat ggaatccggc ccggccagcg gttcaacctt tgtgcgtgtg aagccgacct
     6061 tcttcatcca ctgccggcaa tccgcaccgg tgaaatcaaa tccgcccgga gtctcgatga
     6121 gcatattgag gctcatcaac aaaccaaagg cattcgtttt cctggcgtca tcgatcaggg
     6181 cctcatgaac gatcaatgcg cctcccggtg gcagcgctgc ataggccttg cgcaacagca
     6241 tcattttttc tttgaggtcc cagtcgtgca agatgtgccc catgatgagc acatccacct
     6301 tcggcagcga gtccttgaag aaatctccct cgtgaaacgt catgcgcttc tgcagcttct
     6361 tgcttctgat ataggattca aagaccggcc ggacgacagg aagatccatg ccctggccgt
     6421 tgagatgctg gtgcgccagc gcaatctcaa ccgccacgcc gccttgagca caaccgatgt
     6481 cggcaaacga gtggtatcgt ttccagggaa atttcgcggc gatggatcgg gccgttccct
     6541 tgctgagtcc ggtcatcgcc ttgaggaatc cttccaatcg ctgcggatcc gcgtacagcg
     6601 tgccgaaaaa atcttcgccg gttttcactt cattctgcgg ttgaccggta cgcagcccct
     6661 cggtcagtga tccccaaaat ccatacaggc gggcattaca catttccagc atgccgccga
     6721 catacgtcgg tttggcccga tcgaggaaca gcgcggtctc tggtgtgttg gcatagcgtg
     6781 tcccgttccg tttcaggagc ttaagtgcca ccaacgcatc gaaaaagtct cgcgcacttc
     6841 gcggatgcag tttcaatctg gccgccaagg ctttcccatc gagttggcgc ttggccaaca
     6901 gcgtgaagag tcccagctcc accgccgaca atagagtctt ggaactccaa aacccgaacc
     6961 cgatttgcag gatgtgatca ggaaccaatg gacgaccggc cataatgcca tcctttcgtg
     7021 agaaaaattc tcgagaggct acgcgggaaa tgtcgccctg tcaatttcag ctaccgctcc
     7081 acggtcacgg accgtatctc tttttcctac gccgtatcga gagagataca gcggctcaga
     7141 ttgcgcagga atcgccacgc cgtcacagcc tacgcagagc gatggatctg caattgggac
     7201 gacaaggcag gatccttgct agagcatcgc acatctactt atccgctcat gacgcaaaaa
     7261 ggagatcctc gacatggcta cgtacctcca cgtgaaggca gccctgacgg cactcgtact
     7321 gttcagcatc atgacggcga ccgccggtgc agagcaatgg aacggaccgc aaactcccac
     7381 ggggaccatc tggcgtccgg gtaacatcgc actggggttt gatcccgccg gtcacagcgg
     7441 cactcctccg gcactcgaaa tacgccgccc cctttcaggc accgaagagg tgctcctcag
     7501 cttgcgttcc acctttaaag aggccaccgc taccgtattg gaagtcgaca ccaaacgggt
     7561 ctacatgggc ggggcggcga ccagaacctc cctggtgccg gatggcgtat tcgatctcgc
     7621 cgtaggacgc ggcatcgccg tgggggtcaa cagtgtcacg gaacgactgc cgaccgatta
     7681 tcgattggtg gtgggcggga aggtgctggc tgaagaagtc cgcatcaagc tgatcaagga
     7741 ctgggcggac tatgtcttcc atccggatta ccgactggcg gcgctccctg aagtggccac
     7801 atacatccag agccaccagc atctgcctga catgccaagc gccgccgaag tcgcagccac
     7861 cggaatcagt ctcggcgaca tgcaggcgaa actgcttcgc aagatcgagg aattgacgct
     7921 gcacgccatc gaacaacacc agaccatcgc agcgctgcag gcgcggttgc aacagctgga
     7981 ggaagaccgg tcgtcccagc agctagggag caagtagccg cgatcgattc acatcgttaa
     8041 gagggggcgc agccatgact caattcaccg cactcacaac ggcgttgctg ctggcgacat
     8101 tgaccctgcc ggccgaggcc gggactacca gcctcacgct gaaccaagtc atcaaggaag
     8161 gcaccgctcg ttactacgac caccctcaag agctgaggct ggcagatggg ttttgggctc
     8221 aggccggttc cgacatgaca gccaccattc accttcagca gggatactac ctggataaga
     8281 cccccctgga taccaaggag aagcaataca cgtccactga cggccggaca tacctggtcg
     8341 ccggctgcgt accgaaacac ctccccatct cgggaccggt cgggtcagtg tgggtggcgt
     8401 gcaacgacct cgattatctc aaggataagg tcgtcgaaag ccgtttcgtg tcgtgtctgg
     8461 atgtgttcac caacggcgtg gtgtccggcg tctcaatcaa ggaggtcaac aatcggacgt
     8521 gggaatcgtt ccggttctcc tgccgcgaca tggggccaga cggcagcatg gtgggcgccg
     8581 ctcagaaatc cgacttctta ttcaactatg aacgcgaagg caagctgtac gaaaccacga
     8641 cggccagcgg gcacctcacc ttcggaatcc tcgaagtggc caacggtcta accggcacca
     8701 agagcctgct ccaaatcgcc cttgaacaca ctgccgccgc cgcgcttcag gctgccggag
     8761 tcaatggacg gcacgtcgac gacttcacac tcagcgacaa agttccggac gcctttggat
     8821 taggcggcat gctgagggtc gatcactgga aatgtccgcc cggcatggtg atcaccggta
     8881 tggccattgg ccataacccg gataagaaag gcaatgacac gaagccagtg tacattctgg
     8941 ccgaatgccg gaagctgctg tacaccccat aaggccttac cttaaagccg ttgcacgaca
     9001 ggtgatctga cgcttcacat ccgcgtctgt ttatccactt ccacctcgtt gtgcactacg
     9061 agccgctccc ccaactccgg gcacgccggg gattcaccta gttacgacgt ctcgccatcc
     9121 cggcttttaa tagattatgg ctacaatgct ccagaaggcg ctgcaaggac tcctgctcgt
     9181 cctgctggtg ggctgctcga caacgtatga gccgcatctc gtgcttcctc ctgccgaatt
     9241 caaccgctcc atcgatgcgg ccgtggaagt gcatcccttt gtcgcagctg atgaccttct
     9301 atacgggcgt tccaactatg gcgtggttgc tgatgactat gccaataagc ccccctcaaa
     9361 gttgacgcaa tcgattaccc agcaagtgac ggcggaattg gcggcccatc gggtgtttcg
     9421 ccgggtcagc acctatgatc cggcaccgga ccttatcctg acgggtcgaa tcgaacggtt
     9481 cttcgaacac gaccgccgta aactatggac atttattccg tactattccg acaaactcgc
     9541 cagtcttttc cgtgtgaata gtttcaagac gaatggagaa attcatctca cactcacctt
     9601 gctgaaaccc acgggagaag tggtgggatc gtatgtgggg catgcttcat tcgacgaaga
     9661 ttacacgccc aatggggagg tgcgtcccgg cgatcgactc aatcgcgcat tgggacaggc
     9721 tctcgcgcag attcgtgatg agatgtttgc ggactcgacc ttgacgaaat cacgtacacc
     9781 ccacccgctt gcgcttcagt gatgcttccc ctcgagtttg atgagaccga gcaacccaga
     9841 agatccgcgc agtcactgac cacgctctcc taatccccgc atctcatcca accagctcgc
     9901 acgccgttgc tcattcatac tttcgatatt gccgatgagc gtggctctgg tgggtttgat
     9961 gccacagcat tgaatgatgt tccgctgcag acccttgagg ctatggacgt ggtaccacca
    10021 ccgatacacc catgccggca tgcccatcgt caccacgaca cgcgccgact ttccggcgaa
    10081 taactttttg gccctcgttg catcgggttg atattggaat gccacacccg agcggaacac
    10141 ctgttcaaag aatgctttga gaagtgccgg cattgatccc agccatagcg ggcagacaat
    10201 cacgagatgc cccgcccaac ccatggactc ttctgcccgt ctgatcgaat ccggcgcttg
    10261 ccctttctca aaatcctcgt ttgttcgtag gacaggaacg tccaacattg cgacttcgac
    10321 ccgccggact gtagaccacc gtcctcgcac tcctttgcat attcgtcagc caatgcatgg
    10381 caaaaatgac gagcctgtcc gtcagggtgc ccctggataa tggtaatgcg cttacccatg
    10441 gtgaggcgtg ggtgtcgcgc ttccctctcg tacgatcgga cgaagggatc gatccatctc
    10501 gcggacgggg ccctctggtc tttgctcgat gctctgcggg gctacagtcg gcctttgttg
    10561 aatcttgggt gtcggcggtg cacaagcggc catagagaag agcagtcccg tcacaatcac
    10621 gaaaacgata ctcgttatgg ctcgcattct ccttcctccc tcaccatctt caatcctctg
    10681 cccgacttca tcgttcctcg ctaggcagcg atcaacagtc ctcacccaga tgagcgcaac
    10741 aggtatgcca gaaccgaggc atggcgaagg gcagcaaatc gagcagaatg gctcgatcac
    10801 gaggccatcg tgcgatggcg ctgcggatat tttccgcagt acgaggacca ggagctggcg
    10861 cgaatgtcca cagcagagaa catccctgac tccggaagac ggctgctgct tttatttttt
    10921 gcgacggagg gggagaatga gcaggggcat gcgccgaagg ctggtgccct gtcgccttat
    10981 tccaaccgaa cagaaggagg gtcctatgag ggcaacagaa tatttcgctc gcgggtgtga
    11041 tcccaacaca ttagtcgtca ggcaaatcat ggaggatgcc gtcgtcacga ttcaccccac
    11101 atcgagcgcc atggccgtcg cagaaatcct gagcgaacac agctttggaa gtgtaccggt
    11161 ggtcgacaag gacggaacct tgcgtggcct cgtgaccgag tttgatctgc tcaaagccgt
    11221 cgagcaggaa cgagatctcc ggagcgtgtt ggtgtcggac attatgacca aagacgtggt
    11281 gacgacgacc gaagagacgc cgataatgag tctgattcat ctgctgcagg agaggcacct
    11341 gatccgagtg ccggtcgtga aggggcagaa actagtcggc atggtggcac ggcgagatgt
    11401 cgtctttgcc tatgtgaaag ccagggcaat ctactggccg taatgccgct ctaggacaga
    11461 gcctctcgaa cggcatggcg cttcacgcgc cacagcccta atgggcgacg ctcggcgcag
    11521 aacggtgtga cggctgctgc acctgcgaat tgcgaggcgc agatagtgcg gcagtcataa
    11581 ccatggtgac tatggcgcca ccaaaggccg cggccatgtc cttctgagcg tcccattcgt
    11641 cgccctgcgt tcccagatac atactgccca attccggact gacgatcatc gctacgatcg
    11701 cttcaatcac ctcaaagaat gcgctctgtg caagaatgct gcttaccggc atcacatagg
    11761 accaactccc ctgcactccc gccagccgca ccagcacttc ccgcaaagga tagaccagca
    11821 agagaccata cgaaaaatgg acaagtctat cgaaaggatt ccgggttagt cccgcaatgt
    11881 ctttgagcca gaaaccgaat ggcacttctg cataggtgta atgagcaccg atggcatgga
    11941 gcaccatgaa tacggcgatg agggagtagg acgtgagaga aaatgtaaaa cggcggtagg
    12001 tcaggactag aatcgcgacg agtccgacgg ccaataaatt ttctaacacc caatcatggc
    12061 gattgaccgg gttgatggcc agccagatcc agatgatgcc ataacccaat aacaaccctg
    12121 ccgcaaaccg gctatgcccc gcctccgcat tgcctcctcg ctgtacggtt ttcaccagta
    12181 ttcccgcagc tactctgaaa acgccgcttc ggactccagc gctgtttccg cccctcgtaa
    12241 tgcgcgcagg accagcagca ccgtcatcga aatcagggcg ccatacagcg ccgcggccat
    12301 atccttttgt gcatcccaga tgtctccctg cgatcctaaa taggtcacgc ccaattccgg
    12361 atgcaccgcg cgagccaccc acgattcaat gatttcccac agtccgctca atccaagaac
    12421 ggtcatgacg ggcaagtaat acaccaccca tccccggata ttggcactca accgaaacaa
    12481 ttcctccatg ggatacgcga gcaggaaccc gaaactaaaa tgcacgatcc gatcaaaatg
    12541 attgcgcccg agatgcaaga ccttatccat ccacaacccg accggcactt cggcatacgt
    12601 ataatgcacg ccgatcgtat ggagagtgag aaaggccgtg ataagcgcat aggaggtgtg
    12661 cgacagtgga agcatccggt gcgtcaccac taggagggcg acgaaaaggg ccggaagaat
    12721 actggccagt agccagaatt gcggatcgac gggagcctgt gccatccaaa tcgacacgat
    12781 cagataccag aggagcagac cgattgaaat cagtttgttg cgactcacct gcaggactcc
    12841 ttgtaaaatg gtacaggagg gacgtccaat caaactcccg aaaggattga attactctac
    12901 tggannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggtaca ggagggacgt
    13021 ccaatcaaac tcccgaaagg attgaattac tctactggat gatcttcggt cctgcaacag
    13081 tcctgccggg ccgttcgccc ccataacata gactcgcagc cggccctctt gcacttggag
    13141 aagtacctag cacaagcggg cgtgctgctc tgtcgcacaa ggccacgttc aaagaccgat
    13201 ggcgaggggc atatcgtgat tcggggcaca gctgatgcat ctcgattgga tcaatgacgg
    13261 atgttgctat gccaatgccc ctgctggata ttcggagcct gagcgtggtc gtcgatggac
    13321 aggtgattct cgatcgactg gacctcgcgg ttcaaggccg ggaaatccat gccctagtcg
    13381 gtgccaacgg ctcgggcaaa accacccttg cctacgtcct gatgggttgt gaagggtact
    13441 cgccgagtgc gggacatgtg ctattcaacg gcatggacct tctgcgtctg aaaatgcatg
    13501 aacgcgcccg attaggattg acgcttgcct ggcaggagcc ggctcgattt gaaggcgtga
    13561 cggtgcgcga atatctgagt ttaggcaaac cagaatgcga tcccgaaccg gctttgaagc
    13621 aggtcggtct ggatccagac cgttatctca cacggcgaat ggataaggca ctgagcgggg
    13681 gcgaacgtca ccgaatcgag ctcgcatcag tcctctccat gaaaccgaag ttggccattc
    13741 tcgacgagcc ggtggcagga attgatatgc tttccatcca tcacattatc gaaatcatcc
    13801 gcgccctgaa ggcaagcggc ggctccgtcc tcctgatcac acaccaggaa gaagtggtcc
    13861 tcattgctga tcgagcctcc cagctctgcg gcggacgcat tattttctcg ggcagcccga
    13921 gtgaagccgt cgatcatttc cgcgggcgca gctgtgtgcg atgcgatgga gaggtctgcg
    13981 ggtatgtcag accttgagga actcatgcgc gtcttgccca gggtgggcgc tgagccgtcg
    14041 atcctcgacg acacaggcat tgcccatgtc gtggcccacg ggcatcacat cctgagccat
    14101 cgaaccgtcc cgggccttcg cgtcgacatt caagaaacgc cggaggccat catcggcaag
    14161 atcgtcgtcg aagcaggtgt caccatcgcc cagcccgtcc acctgtgttt cggcctcgct
    14221 catcccaccg ggacacaacg gatcacaatg gatatgcagg tgaacgaagg ggcgcaggtg
    14281 cgagtgctct ctcattgcct ctttcccttc gtccaagacg gccagcatcg catgcaagcc
    14341 gtgatgacaa tcaatcccgg cgcgtcactg acctatactg aggggcacta ccatggtccc
    14401 tacggtggca tgcaggtctt gccgcatgcc acgatcaaga tcggcaaagg ggcccgttat
    14461 ttctcggatt tctccttact ctccggttca gtggggaacc tcgatatcaa ctacctcgtc
    14521 gaagtggaag aggatggact cgccgaactc agcgccaaaa tcttcgcgca ccagagggat
    14581 cgtctcacac taaaggaagc cgtcatgctg cgcggcgagc ggtcacgaag tctgatcaaa
    14641 acccgtgtag tgctggagga tgatgccacg gcagagatca ccggcattac tgaagcctat
    14701 gcaaagggaa ctcgcggcca cgtagattgc atggaagtcg tccaaggcag agctcacgcc
    14761 agcgcaatcc cgatcgtcaa ggtttttcac ccggaggcga aggttacaca cgaagcagcg
    14821 atcggcagcg tggataagaa ggagctcgaa acactgatgg cgcgcggact gaatccggag
    14881 caagcggtcg aactgatcgt aagcggaatg cttcgctagc ctcgacgtct cacgaacggt
    14941 cggactgtct tgatcaagcg gaaggatcgg gggggcactc cggatcttcg atcactgctc
    15001 cgcattcgag gcatcgataa cgaggagggt cgtcgtcgtg ctccgagtgg atgcgatcga
    15061 tgagtcgcat gtgacggcag gtgagggtcc gtgtcatggt cggatcaagt tgaggactac
    15121 gcataacgac ctccccggat cacgcttgac tatgtacctg agaaaatgcg ccccaaccct
    15181 agcagatttc tcaagacttg acacgacgaa ttttccgcag atggaagcca gacatatgct
    15241 gacacaataa ttggtggcct tatggctttc gccgctacat atagagatca ttattctctg
    15301 cgagcggttc acgcaaaggg ctgggaggaa aaagcaggac atttcgccat caaagatgag
    15361 gaatcaggcg tggccgccgg ctcacccgaa gctggaatga agtgtcgtgc ggcttctgaa
    15421 agcgagacac acagaatccc ttctttcacc gcagcacaaa gaaatgtacg tagcctcttc
    15481 agaaatgcct gctcgtccgc tcgagaacgc acgaatggtc cgcagccttc caagagcgct
    15541 gaagaatgga aaacaagatt cagccattca tactgttgat gccgcatttg acggacgagt
    15601 tgcatcattc tggcaggagt ctccatctcc ggagaaagcc acacctttcg caagagatgc
    15661 aagcgcgaca acaatcctcc gactctaaac ctgccaaatg gggcacggtt gagccggtct
    15721 gccaatgtag cgcaggcctg aaactctcca tggagatacc cgatcgtggc cggaatttcc
    15781 gccagcacct cgttcgagga gcggccggtg tggacattcg gcgcaaatcc ataaggctga
    15841 ggagaacagc aggagaagtc cggaccagcc gtctggaccc acgaggtgta cggagtgaca
    15901 gacgtatcga tacggtatcc aagacgaagg agattgcgcg ccacctccgt gtcaaatccc
    15961 caacggccgc ttcggaacgc ccttggagat cgatcaacgt tgtactgaat cgcttcatgc
    16021 aaccgctgca atttttcgta ttgcagggtc gccgggagat tgcagagcat actattgcgt
    16081 ttacttaacg gttccttaaa cggcggagta ttccacggat gacaatgcgc cccgatctcg
    16141 cactccccgg cttccactat ttcccgaagg atgccgacgg cataggagtc ttgcatcacc
    16201 gggtaggtca ccagatacgt cgggatgatg ccgaactcat tgaggagagc ctgcaatctc
    16261 ggcaagcggt atacattctg cgccgtggcc tggccatgcg gaacgatccc ccattgatcc
    16321 tcttcgacat cgatcgtaac gaccagtctc attattcact ccaacgcagt cggcaacagc
    16381 cgtacagttg attttgttac cgcagcagta tcttgagggc ctgctttgca ccatctacca
    16441 acgtgaccgc cgcagcagac gcactgcgat tgtaatagcg cgatgtcacc aaccggcgaa
    16501 gaccggttga ccattcgagc ttatacggtt gcgagccctg caagagatcc accatctgag
    16561 caccatcgca aagtgcctgc tcaatgaccc gatgcaacag gaggaggccc ggactggctt
    16621 tcggcaggat gtccggatcg aacccgggca aataaaagta ataaacgcct tgatacagaa
    16681 acccatagac gcctgcgatg ggccggtcat ccagcaacaa aagatcgatc ctgactcgcc
    16741 cccgctcact caataccctc acgatatccc ggtgaaaccg tcgagactgt tcattcgcaa
    16801 atacgccacc gcccggccgt gagctccacc gtcgttggtg cagggtgatg acggactcga
    16861 aggcgatctc aacatcggca gggctggtgg cacgggtaaa ttgcattcgc cccgcctgct
    16921 ggagacacct cgtctttcgt tgaatggtgt atcgtcggtt gcgtcccagg ctggctcgat
    16981 acgtggggag atcggccggc agccggatga ccgggcaaca gctcgtcgta ctcacatcac
    17041 cgaccttccc tgccttggcg actaatccgt tccacagatc gagcgtggag gattcggcag
    17101 ataagtctga cagcgtcaga agatcccact catcccgagc gtcaccgaac aaatagtggt
    17161 agaggctctg gagcatcgcg agcttgtgct gttcctgcac aatgaggtcg agatatgagg
    17221 aacacacggc ctcgctcccc aacaatctca attctcgaac cgcgaacacc gcgtgtttct
    17281 tggtgcgacg gagatacagt ggcgcaatgc cgaccaatgt ttcgtgctcg tcaaatgcga
    17341 gaagaattcg cagctgactg tcacccaaat agtggagggt ccagtagtac agccattccc
    17401 aggtgagaaa catgctgtgt cccggcatcc tgtccagcaa cgtctcccac gctccagcca
    17461 gccccttgaa ctcctcgggg gtcgtcacca cctgaatggt cagtcgacgc cgctgcttca
    17521 tgggagatac tcgcgcaaca aaggcaaggc cgaggcgaca aactcttcct gtgctttcca
    17581 ggggtcatcg ttctcccggc ttcgaggctc agcggagacc agcaccagag ccccgtcggt
    17641 ccgcccccgc agcaaggcct gtttaatcgt cgccagttta gcttcgaccc ggtccgtata
    17701 actgctgccg ttgatgtgat accaaaacag ggacgggacg ggacgaccac gctcgtcgaa
    17761 aacggttcga ttcaccgcga tggcatcttc ccttctcccc aacatggcag cgatcgcccc
    17821 cgtatgaagc ggagccgtct ccatgcccac cagctctttc ccctgacgtt gcgacttcgt
    17881 gtatgccacg tacacattca ctcgccgtcc gtcagagtcg cgatacgtcc gcataaggga
    17941 atcatcagcg gcggcgaggc tcacccgtga ggcgctgttc aggcggtggt cgaccaccca
    18001 atccccgacg acgcccggga atgtcgccag ctctttcttg agtcccgacg ccccgcgatc
    18061 aagtccatag agaacaagcg tcgcaacgga aagagtgaca caagccagcc accaggcgcg
    18121 attcgaggca tggcgatgag agactggctc agagaaacat ttcttctcct ccccatggat
    18181 agatggccgc aacggaacgt gctccagtct gcccaacaac caggctcccg caaacaggaa
    18241 cgcgaaggcg acccaatcga caaacaagcc ctgcagtatg tggtacggcc cgtgaagatc
    18301 cttgcctccc gcttgagcat agacgcccat gacggtcaaa cgcagccaat tggccataat
    18361 ggcaattgcg accgacgaca ggagcagggt gacgcgggcc caccaggtct gcaaggtcag
    18421 agccgccagc ggcaatccga tcgccagcac ggcaatgagc aggcccgccc cgctgcattc
    18481 tcgggcgact tcaagagtga cggtaggaag aatgatgtcg atgctgttct ccagaatcac
    18541 ggggatgccg agggcgcgca acatggagac ggacatattt gcagtgagga gttgaaatgg
    18601 ccatacaagc ggctccgtca ggccatcgag gaccggcgtc atgaagatca aataggcgag
    18661 cggaaacgcg agtgctctca aataggcgaa cccgcaaagc agcaacacca gtcccgcgag
    18721 aaccaggatc aacgcgacgc ttccggtggt gagaatgccg gcgacttcgc tcgccaggag
    18781 taaggcgatg gcacctacca gccacagcaa gcctgcgccc agcgccggtt caaccggaag
    18841 ctgcttcagc gtgggccact gtagtctgac gagatagaga ctgagcagag ggaccaggaa
    18901 cccatgggaa aagccgtacc ggttccacca caagtccagg aggtcgtgga gtactggcgc
    18961 acagagcagg aggagaggaa ggcagaggcc tgtgattctc cacagggcgg aataattgcg
    19021 gagcagatac gcaggtgtcc tggagtgaac acccgccgac gtcgggcggg ttcctgtagc
    19081 ggaaggagtg acgagagaat tctccacgcg tcggcctcac tgatcatcac cacagcacag
    19141 ttaaccaact actcatccat cttatttgac gccaccatcc gcgctgcttg ctcggcttcc
    19201 ggaaaaccac ccggcaatga cagggcggat ttcagctcgg acaaggcctc ggtctgccga
    19261 ccaattttgg agagcgccac cgcatggtgg taacggacga cgggatgttg tgggagcgcg
    19321 gccgaagctt ctgccaggta tggatacgcc ctatcgaacc ggccctgctt gaaatagatc
    19381 cagcccagcg tatctttaat cgccgggttg gaaggagcca actccagcgc atcccgtgca
    19441 aacccgagtg catcgtcaag ctcctgctgg tcgctcaggt tcgccgccag attattcgcg
    19501 gcaaccacat cccgaggatt gagctggaag gcgcgtttgt aatggccatt cgccgcatcg
    19561 acttgtcctc gacgggccaa caccatgcca agtgccgtat gaatcgccga attcgtctcg
    19621 tcctttttca aagcactttg aaacgccgac acggctgcag gcagcttatt ctgcagaagc
    19681 tgcacttgtc cgagttcgta gtgcgccagc gcaagatccg gtgccagatc caccgctttc
    19741 cgaaacgctt tctgtgcctg gtcgattttg ccctgagcga cgtacacgcg tcccgcgagt
    19801 ctccatgtgt ttggatcgcg cgatgttttg gccagatacg ccccgagcac gcgatctgcc
    19861 tgctgagact gcttcgaccc cacgagtgtg accaggtagt cctgcatgat caccgggtca
    19921 tcaggcgcat agaccagcgc ttgcgcaaag agctccgcgg cctcagccgg ccttgcttgc
    19981 agctggcgaa gcgctgccat cttcacatag ccaatcggtt gtccaggcag ttgcctgctg
    20041 atggcgccat acacctcctc ggccctggcc cagttcttcc cgaacacgaa cacatcacca
    20101 tacaacacgg cggcttccag gctggcgggg ttcactcgca acgtctgcga gacttccttc
    20161 tccgcctcct tcggcttctc ttgtgtgaga taaagacgcg cgagagccag atgcgcagtc
    20221 tggttagagg gatcacgcct gatcgcctcg cgcagcgctt cttctgcctg gtccatgcgg
    20281 ccttgggttg atctaatcaa gccaaggtac acatgaggcc cgacgagagt cgtatcacga
    20341 ccgatcgccg cttcgaacag gctggccgct tggagcagat cacgttccgc gaatgccacc
    20401 cgccccttga gatacagggc gaccggatca tgttcatcgg ctgcgagaat cgcctgcagg
    20461 agcggtttcg cttcggctac ctcaccaagg gtcaacttca gctcggcgag ggccttccgt
    20521 gccctctgag aatgcctgtc ggcaatgagc gcggacaatt cgtgttcggc atcttcccga
    20581 aggccgagca tggcataccg ctccgcgagc actagcttga tggattcctg atccgaactc
    20641 agctcgactg ccttgtgaaa ctccttccgc ccctcctccg gagcaccggt cgcaaacaga
    20701 aactgtccac gagcaatgcg agcaccggca gatgcaggat cagccttgac cccgcggtcg
    20761 taccactcgg ccgcctgctc gatgtcttgt tgtgcaaaat ggatattcgc cagggcgagg
    20821 tgggggccca ccatcttcgg atcgagtgtg accgcctgct tcaggatcgc gattgcatct
    20881 gcattccgct ctgctctcaa ggcgactgct gcgcgaagca ggtaccctgc cgggtgttgg
    20941 ggattggccg acaccagctc ttcggcaatc ttgcctacct cgtccatttt ccctccggcc
    21001 agatacagat cgccgaggga ccatttcgcc tcgaaatgcg acggatcaag ttgcaccacc
    21061 cgaccgagcg cctggtacgc tgtggatggg tctccgccct ttaacgccgc cttcgcgagt
    21121 ttccactgga gagaaggatt atccggagca gcccgcacgg cgttcttaaa ctcgatcatt
    21181 gcttcgcgga actgttcctg ctgcacatac ccgtcaccgc gcacctcatg cttcgcagcc
    21241 tgctc
//
LOCUS       DAOQDS010000061        21235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_937_length_21235_cov_21.111662, whole genome shotgun sequence.
ACCESSION   DAOQDS010000061 DAOQDS010000000
VERSION     DAOQDS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 21235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21235
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_937_length_21235_cov_21.111662"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3686
                     /locus_tag="PK589_14165"
     CDS             <1..3686
                     /locus_tag="PK589_14165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ20802.1"
                     /translation="LDISARKELEAAQALAQRDLQVKQQALDEAQTLAHLGFWEWDIV
                     TGDEQWSNEQCRIFGFEPGTVKPTYATFLSALHQDDKSRVQQAIDAAITYDFPYNIDC
                     RIHRPSGEVRHINCRGTVRRKPDGSPLAMTGTVQDITAHKHAELAWRESEARMRSIFE
                     SAIEGIVVIDEKGRIESVNAALLQLLGYEAHELIGQNISLIMPEPYRANHSHYLANYL
                     VTGKRVIIGSGREVTAVRKDGTLIDIHVSVSEMQIGPSKKYTGMIRDISERKRMEETI
                     RESEARFRQLAESIDAVFWLTTPDKSEVLYVSPAFETIWECPRDILRTNPLFWLEHIH
                     PEDQKRVSVAAACQAHLPYDEEYRIITATGRERWIRDRSFVIKNEAGQTYRIAGIAQD
                     ITESKQLEAQLRASELRHRSLVELSPHAIFVNCDDKIVFANQACVKLFGAFASSQIVG
                     KRVIEFIHPDSQTIAQERIARLRSANGSVPPIEERFVALDGSILDVEVAAATIRFEEK
                     PAIQVILTDIRARKELERALVATNLQLEAILASATKVSIIAINTAGIITTFNAGAEEM
                     LGYSAEEMIGQPMLTALHLPEEIDKHTRDMSELYGASFQRLEALTQQARLGGFDEQEW
                     TYRRKDAQRLTVLVTITALKNTDGVVTGFLAIGKDITQRKEAEAALTEAARELARNNV
                     ELAQARDEALQAAQAKADFLATMSHEIRTPMNAIIGMTGLLLDTALTEEQHDFAETVR
                     RSSDALLTIVNDILDFSKIEAGKLNFEEIAFDLRLAVEDTIELMAEQAQSKGLELISL
                     VDAAVPTGVLGDPGRLRQILVNLVSNAVKFTTTGEVFVHVTRETGESPNHLRFAITDT
                     GIGISDEAQKKLFHAFVQADSSTTRRFGGTGLGLAICQRLVQQMHGRIGIDSRPGEGS
                     TFWFTVALPEAALSPTSFALSWDRLSGRRILVVDPNNRMRKVLQEDLAAKGLQCSSAS
                     NAGEALDLAQAAAAMQKPFDLALIELHLPDKDGFETAALLKQNSTTSGMRLVILTTVG
                     RRGDGTTARQLGIDAYLTKPLRQTQLLECFCQLLDVAQPDVASDTASTGTPPLITRHT
                     LSQTPTTSTTRLLLAEDNPVNQKVACKMLEKLGYRVDVASNGQEAVAAHERMRYPLIF
                     MDCQMPEIDGFEATALIRKMEGKSAHTPIVAMTANAMQGDRERCLEAGMDDYVAKPVR
                     PKDLQTVLDTWLGNRADKTGTTG"
     gene            3701..3967
                     /locus_tag="PK589_14170"
     CDS             3701..3967
                     /locus_tag="PK589_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20803.1"
                     /translation="MSVHPLRLRHRHEPALDSSILHVLPANIAVLGADGTILAVNEAS
                     ERFATANGFTENAHAGIRHVPDRTFGCSRARVRTPQLVSAAMVS"
     gene            4057..6489
                     /locus_tag="PK589_14175"
     CDS             4057..6489
                     /locus_tag="PK589_14175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ20804.1"
                     /translation="MHRSQAGLMAILEAAVDGIVVINERGIIESVNPAVTKLFGYTPD
                     RLLGQNVKMLMPDPYHTEHDQYLRNYLTSGLKKVIGIGREVLGRRSDGTSFPIHLAVN
                     EILVDGSRKFAGIVRDISSQILSEMQFRQVVDSAPNGLLMIDPHGTIMMVNKQVEAMF
                     GYSREELLGNRVEMLMPERFRSNHSTYRRGYFSGPTSRAMGVGRDLPGLRRDGSEFPV
                     EIGLNPIDTPQGRLALASIVDISGRKRMEDALAKAAEDLEEKNRELSETRDMALRAGQ
                     AKTDFLATMSHEIRTPMNGVLGMTTLLLDTTLTAEQRDYLQTLRSSSESLLRIINDIL
                     DFSKIEAGKFTIEHIPFDLRLTIEDTLDILAPTAQGRQLELVGLIDAQTPRTVVGDPG
                     RIRQILTNLVGNAIKFTEHGEVLIQVLQTDEDPGSVVLRFEVIDTGIGLSPEAQAKMF
                     QAFTQADSSTARKYGGTGLGLTICKRLVELMGGTIGLQSFAGQGTCVWFTIRLGTHTD
                     AAPAVPPIPSGLDLTGLRVCLVDDNATNRSLLQYHVSAWNMHHESAVDGPSALALLRR
                     GVEEGTPFDVAIIDMYMPEMNGLELCRLIKQDPALRKTHLIMLTASGQRGDSLAAQEA
                     GAAAYLTKPIRERHLADCIRLIFGRDNAKEQPAPFLTRHTITEAIAREAPRLLVVDDN
                     PVNQKVAVKMLEKLGCRVDLAGNGMEALAAICRHQYPLVFMDCQMPDLDGLETTRLLR
                     SQEKPGEHLPVIAMTANAMEGDRESCLKAGMDDFISKPIITSDLKKILARWVPQNQLE
                     GEQAASPETPSS"
     gene            6634..7191
                     /locus_tag="PK589_14180"
     CDS             6634..7191
                     /locus_tag="PK589_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNJ20805.1"
                     /translation="MSHPSPSHTPSAPSTAAHRVEQLFEILIFGSRWIQAPLYGGLIV
                     AELLYAAKFLTELWHMIVHFREESETLFMLGVLSLIDVTMVANLLTMVIIGGYATFVS
                     KLDLETHPDRPEWLTHVDPGTIKIKLAASLVGISSIHLLKAFVDVTNENPEHIKWKIF
                     IHMTFLGSAIFLAYTDKLMQRDRKH"
     gene            7458..11456
                     /locus_tag="PK589_14185"
     CDS             7458..11456
                     /locus_tag="PK589_14185"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNJ20806.1"
                     /translation="MTTPGPEKDTYLTVANILGEETELLHGPFSDDHPLKVLERAAPT
                     EASDKRYLDELNRHIHACHPKRAGLCLSGGGIRSATFGLGVLQSLARLQLLDKFDFLS
                     TVSGGGYIGSWLTAWIHRHPRGLKGVIEDLNRPSKAGSEEASLPVRWLRNYSNYLSPH
                     LGFFSADSWTLLGIYLRNLHLNWMVLLPLLMMPLLAPRWIIALAQLNAKETSPSPISA
                     ASLLVIGIGLAVMALIYLHLCRPTLREFRRDTWWQTLESQRWFLIACLCPLLISVTCL
                     TTAWAWFRNGGGGADQFTWAHAVFGGALIHTVSWLFSAVALNRFRAFTRWLLLETLAV
                     AFTGALGGWLLRASFIKTCGQWNVASHAEWFATLAVPGVLTIFLLTATIFVGLASRFT
                     EEQDREWWGRTGSWVLIAMVMWSGLCAIVIFGPGLIHWMTPQVAASVGGLSGLLTLFL
                     GFSSRTTAQQQEERSTVTAVTDLAVRAAAPLFMVCVLIALALGTSWQLDLLAHHYHMH
                     LNHLDGPMQTGLGIGSDPWGHDQILHNTPWWLLFRFTATVVALGLLMSVFININRFSL
                     HAMYRNRLIRAYLGASRRQAERERTFNPFTGFDNLDNPSVSDLRFDDVRLARWFSAQE
                     QKQPTMQALLSYLQLSNPTDEQQEALCAQLPQDLRQTQACKRLSEAAHQLRLGPACVA
                     KLTALQMPDTAAGREASKWFSDLFIHKLHAQNPGPFHVINIALNLVKGDNLAWQQRKA
                     QSFTISPLHSGSWNLGYRRSEEYGANRYIGQPLSLGTALAISGAAASPNMGYHSSSAV
                     TFLLALFNIRLGWWLGNPGRAGADTYHQASPTLSIRPLLAEAFGLTDSCHPYVYLSDG
                     GHFENLGLYEMVLRRCHCILVVDAGCDPRLTFEDLGNAIRKIRIDLGIDIELNLDQIK
                     RAEGATTSNRHHAIGTIRYDKVDDNATAGTLIYLKPSLTGNEPSDVQDYAARHSAFPH
                     EPTSDQFFDEAQFESYRRLGEHIAQEVLRPALHRTEQDFSRLCHALRSHWVTTPPGMQ
                     ESFLGETDDLKDLERLLRDDPDLLRYDMEIYPELCSVFGLDCGNVALNPRAALHTCNL
                     QIQLMEQVYLAVKLEEFHGHALNRGWMNLFRRWTSSATFRMFWPTVCSMYSQPFVRFA
                     GQHFNLSIDQVAVLQPLEQTGDLSSLFRELLIEWASVPDYAETFMNALKRPLPLEEVT
                     DGPPPPLAAWQMRLPAIDSQAGPVGSGEILAVVAVTRLSVTGHRLALYGWVRPAYRGL
                     GLGHRLFGKAIDVLTEVYSGHNLIVDLGPDNAAWAGTSIRNVGWLRFYEQLGFTRDRS
                     DPKRFQMMRELS"
     gene            complement(11487..11870)
                     /locus_tag="PK589_14190"
     CDS             complement(11487..11870)
                     /locus_tag="PK589_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ20807.1"
                     /translation="MPLILIADDNQQICGWLRAVLEAEGYRVIQASDGREAMATIERD
                     APDLMILDMYLPVQDGLETILMLHNVQSSVKILGISGQPLQGCDILKIAMAFGAHGVL
                     EKPFSRELLLRQVQTLLSSVQLPSV"
     gene            complement(11889..12569)
                     /locus_tag="PK589_14195"
     CDS             complement(11889..12569)
                     /locus_tag="PK589_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ20808.1"
                     /translation="MEQQSVYRIVLIDDSRMVRLGIREQLLTATDFRVVGEAMSAVEG
                     MELVEQVKPDLVLLDSRLPDLSGVEACRTMVARNPAQRVLMLSIQDDPVVVQEAIVAG
                     ARGYLLKDAPVDTWLDAVRRVASGDVLFQPHLLGMVLREVHDMGNGLSSKSLAALSPQ
                     EYRLLPLVAEGKTNKEIASALALSDKTVKNYLANIFSKLGISRRTQAATLYARALPSR
                     SMSSGHYV"
     gene            complement(12725..13363)
                     /locus_tag="PK589_14200"
     CDS             complement(12725..13363)
                     /locus_tag="PK589_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ20809.1"
                     /translation="MNKCRCLIADDHPVVRRGVRDLLEDEQLCSEILEAETGEEALDA
                     VRRQPWDLMILDIALPDKHGLDVLKEAKLLQPRLPVLMLSLYPEKEFAMRAIRAGASG
                     YLTKQSAPSELLAAVSRVLQGGRYITAALAEQLAQAFETGTEGELHARLSDRELQVLR
                     LLGQGKSVSMIGEELCLSVKTISTYRARILEKLSCESTGELIRYAIEARLID"
     gene            complement(13356..19316)
                     /locus_tag="PK589_14205"
     CDS             complement(13356..19316)
                     /locus_tag="PK589_14205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ20810.1"
                     /translation="MGTHTLPPDETARVGALCRYRVLDSPPEADFDEMVQLAASIYKA
                     PIAAITFIDHDRQWVKAAVGLDTSQTDRPSGLCSFTIQGKDPMVIEDASADDRFSSHA
                     LVRGHPHVRFYCGAPLVTRDGYAVGTIAVMDSIPRTVKEDDLLTIRRLARQTAALLEL
                     RMLRSERSSVDSLSTDDQVETLTEAAFVIDRSGRILRANRGAERLLGVLASDLRGRLL
                     HECVHRPGDGDEEGQDGSCTLMHACLSGTAVLEQDDVFVNGDGSLLQVRWNHVPCWSG
                     GDWSGGIVAVRDLTIQKRNEEVLRLMAPTTRLTGTAFLQDLVETLVKTIGADYGFCLR
                     ALPEGRCAHSVAGWGPNGRLDPVDYALAGTPCEHVLHEGFCHYSSGVQALFPQDEVLV
                     HLSIESYMALTLPSPTGGPVGWIALLGRSPLKDAERAEALLRVAAGRAGSELGRHLAE
                     EQLKESEERYALALRGTSDGIWDWNVLAGQAVLSPRWKALLGFAEDELPDHESSFFQR
                     VHPEDVPRVKAEIERHFARRTPYDVQCRLLHKDGTYRWVQTRAQALWDDQGRAYRMVG
                     ASTDITERKQAEDVARRWQQVFDQAQLGLAHADLATNTFLAVNPAFARQCGYTVEELI
                     GRSIFFIYPPEEHAALEERIQRIHDTGGLTFESIHRRKDGSLFPVMVEVMLIRDADGV
                     PVSRVAYVKDITEAKQAEQARRASEERFRAAYRNATVGMSIADVGGRIQEVNQALCKI
                     LGYSEQELLANTFQSITHPDDLGQNLDRIQTLLSGRRVYDVFEKRYVRKDGGVVWAQV
                     GVSVIRDEEGTPLYTLAMVQDITERKHAQEALQLAKYTVDSATEAIYWVGADAELLDV
                     NDSACSMLGYSKDEFRRMRVHDINPNFQAETWPTFWAHTKERGRVSFESCHRAKDGRL
                     IPIDVNVNFVHYNGVECHFAFVRDITERKSAESALRASEERFHFAIEATNDGMWDWDM
                     RTDEVYYSPQWIRLLGYQSEEVLPTRAFFMTLLHPDDVARMMDVLQAHVDGRIPLKEI
                     EVRLRQKSGEFRWYLNRGKVVARAEDGRPLRMVGTITDISERKQSERERDEALSHLET
                     IMETVPDIIFALDLEGRVAKWNRKLETVTGYTREELQGKSALEMVPSDEAEKTAAAIR
                     EAYEKGYAELEGHLLAKDGRTMLYHWTGAPYRDLQGRVIGVTGVGRDITERKKVEQML
                     ASETRILEMMGSDAPLSAVLAEICLMIEGLSSGVLCSVLLLEEDEAHLRHGAGPSLPA
                     SYARILDGMAIGPARGSCGTAAFTGQQVVVTDIASHPFWADFRELALAHGLRACWSTP
                     IISAAGKILGTFAIYNQTPRQPTAEAPIVARASHLACLAIERKRTESALRLMQFSIQQ
                     AVDAVFLVSAEARILDVNEAACAMLGYTREELLSKTVHDIDTNFTRENWRARWQHLKV
                     RKSFSYESRHQKKDGTVIQTDTTINFLVHEGREYYCAFMRDITNRKRAEAQLHLTQFA
                     MERAADMVFWVDEAARLLLVNAAACERTGYTKDELLHMAVPDIDPNYQMEAWVRHWQE
                     LRETGRQRLETQHRSKSGEIYPVEVVANFVVFEGREYNFATVRDMSERQRTELRLRSS
                     EERFRLVAQATNDVLWDWDLVTNAHWWSPNAREKFGYDPQREPSIDAWTVRLHPDDKA
                     PILAMVQQAIESEVQTLAAEYRFLLADGTYGYFLDRAHIVRNEVGMAIRMIGAMIDVT
                     GPKRAYASLEEAYRRFQAMSQELQMVESNERRRLSRELHDELGQLLTSLKFDLASVKR
                     SVASRSKALGPRSQERLARALETTDLLFVRLRQIVRALRPPVLEELGLKAALEALIAD
                     VQARTRLRCSLVFEQGEGGPSRCPTLETAFYRIVQELLTNVIRHARATTVAIAITSGQ
                     QNWQMTVQDDGIGFDVAGLAPTGGFGLRGIRERVEILGGQVDILSSSETGTRVHVVIP
                     VSSEPEPACETQHETTASRRRRRKTVDE"
     gene            complement(19463..>21235)
                     /locus_tag="PK589_14210"
     CDS             complement(19463..>21235)
                     /locus_tag="PK589_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ20811.1"
                     /translation="QTPPMTKVGVLDVESFFPSKERFALAMQLNNLLAAPGFAAWMEG
                     EALDVGQILYGPSGKPRIAIFSIAHLNDAERMFFVALLLNQTLGWVRSQSGTTSLRAI
                     LYMDEIFGYFPPVANPPSKAPLLTLLKQARAFGLGVVLATQNPVDLDYKGLANTGTWF
                     IGRLQTERDKARVIEGLEGAAASSGKKFDRQQMEQILAGLGNRVFLLNNVHEDAPEVF
                     QTRWTLSYLRGPLTRTQIKQLMDPVKRQLPARTSRQDELGAAGASGSTSAASRTTASR
                     PMLPPDVPQYFLPLRGTQPSGATLIYQPMVLGAAQVRFTDNKAGIEASTDLTRLAAIS
                     SGVVPVEWEQSTAADVQVADLEREPAADAEFAALPGAASKGKQYDSWKSDLSGWLFRT
                     QKLELWRSPTTNTLSKPGESERDFRVRLQQVGREERDRLSDELRKKYAPKITTLRDRV
                     RRAEQAREKQQNESRSSQVQAVISVGASILGAFLGRKTLSATNIGRATTAIRSAGRVM
                     KESQEVGHAEENVATLQQQLTDLEAQFKSESDALMAATDPLTEKLDQISLKPTKSNII
                     VKLVGLAWVPAWRGADGTSAPAWL"
BASE COUNT         4428 a         6616 c         5837 g         4354 t
ORIGIN      
        1 atctcgatat ttccgcgcgc aaggaattgg aagcagccca ggctctcgcc cagcgagatc
       61 tccaagtcaa acaacaggcg ctggatgagg cgcaaacatt ggctcatctc ggcttctggg
      121 agtgggacat tgtcacgggg gacgaacaat ggtcgaacga gcaatgccgc atattcggct
      181 ttgagccagg gaccgtcaag ccgacctatg ccacgtttct ctccgccctg caccaggacg
      241 acaagagccg cgtccaacag gcaattgatg cggcgatcac ctacgacttc ccctataaca
      301 tcgactgtcg gattcaccgg ccgagcggcg aagtgcgcca catcaactgt cgtggaaccg
      361 tccgccgaaa acccgacggt tctcccctcg ccatgaccgg aacggtgcaa gacattactg
      421 cccacaaaca cgccgaatta gcatggcgtg agagcgaagc ccgcatgcgc tcaatcttcg
      481 aaagcgcgat agagggtatc gtcgtcatcg atgaaaaggg ccgcatcgaa agcgtcaatg
      541 cggccctgct acagctgttg ggatatgagg cgcatgaatt gatcggccag aacatttcgc
      601 tgatcatgcc tgagccctat cgcgccaacc actcgcacta tcttgccaat tacctggtca
      661 cggggaaacg ggtgatcatc ggcagcggcc gcgaggtgac cgcggttcga aaagacggca
      721 ctctcatcga cattcatgtg tccgtcagtg aaatgcagat cggcccctcg aaaaaatata
      781 cgggaatgat tcgggatatc tccgagcgca agcggatgga agaaaccatc cgtgagagcg
      841 aagcgcgctt ccggcaattg gcggaatcga tcgatgcggt cttctggctc accacgccgg
      901 acaagagcga ggtgctgtac gtcagccctg cgttcgaaac catctgggaa tgtccgcgcg
      961 acattctccg taccaatccc ctcttctggc tcgagcacat ccacccggaa gaccaaaaac
     1021 gtgtatcggt cgcggcagcc tgtcaggcgc atctgcccta tgacgaggaa tatcggatca
     1081 tcacggctac gggacgtgaa cgctggatca gggaccgcag cttcgtcatt aaaaacgaag
     1141 caggacagac ctatcgcatc gcgggcatcg cgcaggacat caccgaatcc aaacagctgg
     1201 aggcacaact gcgcgccagt gaactacggc acagatctct ggtggaactg tcgccccatg
     1261 ccattttcgt caactgtgac gataagatcg tgtttgccaa ccaggcctgc gtgaaactct
     1321 tcggggcatt tgcttcttcg cagatcgtgg ggaagcgcgt aatcgaattc atccatccgg
     1381 actcacagac gattgcccaa gaacgaatcg ctcgtctccg gtcggcgaac ggcagcgtac
     1441 ccccgataga ggaacgcttc gtggcgcttg atggttccat cctcgatgtg gaagtggctg
     1501 ccgccaccat ccggtttgaa gaaaagccgg ccatccaggt cattttgacc gatattcgtg
     1561 cccgtaaaga gctcgagcgg gccttggttg ccaccaatct ccaactggaa gcgattcttg
     1621 ccagcgccac caaagtgtcc atcatcgcga tcaacactgc tggcatcatc acaaccttca
     1681 atgcgggcgc ggaagagatg ctcggatatt ccgctgagga gatgatcggg cagcccatgc
     1741 tcacggccct gcatctaccg gaagaaatcg acaagcatac ccgcgacatg agcgagctat
     1801 acggcgcttc cttccagaga ttggaagcgt tgacccagca ggctcgactg ggcgggttcg
     1861 acgagcagga atggacctat cggcgaaaag atgcgcaacg cctcacggta ctggtcacca
     1921 tcacggccct taaaaacact gacggtgtcg tgaccggatt cctggccatc ggaaaagaca
     1981 tcacccaacg gaaggaagcg gaggccgccc tgactgaggc cgcccgcgag ctggcacgga
     2041 ataacgtgga actcgcccag gcgagagatg aggctctgca agcggcccag gcgaaagccg
     2101 acttcctcgc caccatgagc catgaaatca gaaccccgat gaatgcgatc atcgggatga
     2161 caggacttct gctggatact gccctcaccg aagaacaaca cgacttcgcc gagaccgtcc
     2221 gccgctcgag tgatgcgctg ctgaccatcg tcaacgatat tctcgacttc tcgaaaatcg
     2281 aggccgggaa actcaatttt gaagaaatcg cctttgatct tcgattggcc gtcgaagaca
     2341 caattgagtt gatggccgaa caagcgcaga gcaagggcct cgagttgatc agcctggttg
     2401 atgccgccgt ccccaccggc gtcctgggcg atcccggccg gctccgacaa attctcgtca
     2461 acctcgtcag caatgccgtg aagttcacca cgacgggcga ggtgtttgtc cacgtcactc
     2521 gagagacggg agaaagcccg aaccacctgc gtttcgctat tacggataca ggcatcggca
     2581 tctcggatga ggcgcagaaa aaactgttcc acgctttcgt ccaggccgac agttcgacga
     2641 cccgccgatt cggaggaacc ggtctcggac tcgccatttg ccaacgcctc gtccagcaaa
     2701 tgcatggacg catcggcatc gacagccggc ccggcgaagg gagcaccttc tggttcaccg
     2761 tcgcactacc cgaagcagct cttagcccta cgtctttcgc tctttcatgg gatcggctca
     2821 gcgggcggcg cattctcgtc gtggatccga acaacaggat gaggaaggtg ctgcaagagg
     2881 acctggcggc caaaggtctg cagtgcagta gtgccagcaa tgcgggagag gccttggacc
     2941 tggcgcaggc ggctgcagcc atgcagaagc ccttcgatct ggccttgatc gagttgcacc
     3001 tgcccgacaa agacggtttt gagacggccg cgttactgaa gcagaattcc acaaccagcg
     3061 gcatgcgcct ggtgattttg accaccgtcg gccggcgggg agatggaact accgcgcggc
     3121 agctcggcat cgatgcctat ctcaccaaac cgctgcggca aacacagttg ctcgaatgtt
     3181 tttgtcagct gctggatgtc gctcagccgg acgtcgcgtc agacacggct tccaccggca
     3241 caccgccgct gattacccgg cataccttga gccagacccc gacgacgtcg accacacgcc
     3301 tgcttctggc agaggacaat ccagtgaacc agaaggtggc ctgcaagatg ctggagaagc
     3361 tgggctatcg cgtcgatgtg gccagcaacg gtcaggaagc cgtggccgcc cacgagcgga
     3421 tgcgctatcc gctgatcttc atggactgcc aaatgcccga aatcgatggc ttcgaagcga
     3481 ccgcgctgat ccgcaagatg gaaggcaaat ctgcccatac gcccatcgtc gccatgacgg
     3541 ccaatgccat gcagggagat cgcgaacgct gtctggaagc gggcatggac gactacgtcg
     3601 ccaaacccgt caggccgaaa gaccttcaaa ccgtgctgga tacctggctt ggcaatcgcg
     3661 cggacaaaac cgggaccacc gggtaacaag gaaatccatc atgtccgtcc atcctcttcg
     3721 tctcagacat cgacatgaac cagcgctgga ctccagcatc ctgcatgtct tgccggccaa
     3781 tatcgccgtg ctgggcgccg atggaaccat tctcgccgtc aatgaagcct cggagcggtt
     3841 cgccacagcc aacgggttca ccgagaacgc ccacgcgggg atccgacacg ttccggacag
     3901 gacattcgga tgttctcgcg cgagagtccg aacgcctcag ctcgtttctg cggcgatggt
     3961 ttcgtagcaa tcgtcacccc tatgcagggg cagtaggact atggcgcggt ggtcaagcat
     4021 atcaatgtgt cccacttcaa gcaggagtaa gccgccatgc atcgcagcca agcagggttg
     4081 atggccatcc tagaggcggc ggtggacggc atcgtcgtga tcaacgaacg gggcatcatc
     4141 gagtctgtca accccgccgt cacgaaactg ttcggctaca ctccggaccg gttgctcggt
     4201 caaaacgtca aaatgctcat gcccgatccc taccacaccg agcatgacca atacctgcgc
     4261 aattacctga catccggcct gaagaaagtc atcggcatcg gtcgtgaagt gctgggacgg
     4321 cgaagcgatg gaacgtcgtt tccgattcat ttagccgtca atgaaatact ggtcgacggg
     4381 tcgagaaagt ttgcaggaat cgtccgggat atctcgtctc agatcctgtc cgaaatgcag
     4441 ttccggcagg tggtcgactc ggcccccaac ggcctgctga tgattgatcc tcacggcact
     4501 atcatgatgg tcaacaagca ggtcgaagcc atgttcggat acagccgcga ggaactattg
     4561 ggaaaccgag tggaaatgtt gatgccggaa cggttccgat caaatcattc cacctaccgt
     4621 cgcggttatt tttcggggcc tacctcccgc gccatgggag tggggcggga tcttcccgga
     4681 ctgcgtcgag atggctcaga gttcccggtt gaaatagggc tcaacccgat tgacacgccc
     4741 caaggccgac ttgctctcgc gtccatcgtc gacatttcag gacgcaaacg gatggaagat
     4801 gcgttagcca aagccgccga ggaccttgaa gaaaaaaacc gcgaattgtc ggaaacgcgc
     4861 gacatggcgc tgcgagccgg ccaggccaag acggattttc tcgcgaccat gagtcacgag
     4921 atcaggactc cgatgaatgg cgtgttaggt atgacaaccc tgctgctcga caccaccttg
     4981 actgcagagc agcgggatta cttgcagacg ctcagaagtt ccagcgagtc cctcctgcgc
     5041 atcatcaatg acattctgga cttctcaaag atcgaagccg gcaagttcac catcgaacat
     5101 attccgttcg atctccggct cacaatagaa gatacgctgg acattctggc gcccaccgcg
     5161 caaggacggc agctcgaact ggtgggtttg attgatgcgc aaactccccg cacggtcgtt
     5221 ggtgatcctg gccgcatccg tcaaattctc accaacctcg tcggcaacgc aatcaagttt
     5281 acggagcatg gtgaagtcct gatccaggtt ttacagacag acgaagatcc aggctcagtc
     5341 gtcctccgat ttgaagtgat cgataccggc atcggcctga gccctgaggc gcaagccaaa
     5401 atgtttcagg cctttactca agccgatagc tcaacggcca gaaaatatgg cggaacgggt
     5461 cttgggctga cgatttgcaa acgtctcgtc gagttgatgg gcggaacaat cggcctgcag
     5521 tcattcgccg ggcagggaac ctgcgtttgg tttacaatcc gccttggcac ccacacggac
     5581 gcggctccgg cggttcctcc catcccctca ggcctggacc tcaccggcct gcgggtctgc
     5641 ctggtcgatg ataacgcgac caaccggagc ctcctccagt accatgtgtc ggcgtggaac
     5701 atgcaccacg aaagcgcggt cgacggcccg tctgcattgg ccctcctgcg gagaggggtg
     5761 gaagaaggga cgccgttcga tgtcgctatt atcgatatgt acatgccgga gatgaacggc
     5821 ctcgagctct gccggttgat caaacaagat ccggctcttc gaaaaacgca cctaatcatg
     5881 ctcaccgctt cgggccaacg tggagacagc ctggctgccc aggaggccgg cgccgccgca
     5941 tacctgacga aaccaatccg cgaacgccac ctcgccgatt gcatccgtct catcttcggc
     6001 cgcgacaatg cgaaggaaca gccggctccg tttcttacgc gtcataccat cacggaagcc
     6061 atagctcgcg aggcgccacg gctgctggtc gtggacgata acccggtcaa tcagaaggtc
     6121 gccgtgaaga tgctcgaaaa attgggatgc cgcgtggatt tggcgggaaa cgggatggag
     6181 gcactggctg ccatctgccg acatcagtac ccgttggtct ttatggactg tcagatgccg
     6241 gatctcgacg gcctcgagac cacccgattg ctgcggtccc aggaaaagcc gggcgaacat
     6301 cttccggtca tcgcgatgac agccaatgcg atggagggcg atcgcgaaag ctgtcttaag
     6361 gccggcatgg atgactttat cagcaaaccg attatcacct ccgatctgaa gaagatcctc
     6421 gcgcgatggg ttccgcagaa ccaattggaa ggtgagcaag ccgcctcgcc tgaaaccccg
     6481 tcgtcttgat tgccccgttc ccccaagacg ctcctcatcc agccggcgcg cccgacttca
     6541 tacaagcttc gcgtcctctc tccctatctc gcgattgaca gaagccggcg cgcccactag
     6601 gatacgccct caacaaggag ggtacgtgca cgcgtgagtc atccgtctcc atcccatact
     6661 ccatccgcac catctacagc agcccatcgc gtcgagcagc tcttcgaaat cctgatcttc
     6721 gggagccgat ggattcaagc tcccctctat ggcgggctga tcgtcgctga actcctctat
     6781 gccgccaagt tcctgacgga gctctggcac atgatcgtcc atttccgcga ggagagcgaa
     6841 accctgttca tgctgggcgt gctgtcactg atcgacgtca ccatggtcgc caacctcttg
     6901 accatggtca tcatcggcgg ctacgcgacc ttcgtcagca aactcgatct ggaaacccat
     6961 ccggaccgcc ctgaatggct cacccatgtg gaccccggca ccatcaagat caagctggcc
     7021 gcgtcgttgg tcggtatttc cagtattcac ctcctcaagg cttttgtgga cgtgaccaac
     7081 gaaaaccccg aacacatcaa gtggaagatc tttattcaca tgaccttcct tggttcggcg
     7141 atctttctgg cctataccga caagctgatg cagcgggacc ggaaacacta accgcacctc
     7201 tggaaaggtt ggggcatcct gttccgtcgt atcaattgag atacgggtgg tatctattcc
     7261 gataatcggt cacccatgtc gtcagacact gtcccttgta tccgccgcct ctccccagcc
     7321 attaacgacc ctgcactgtc gcttcgaatg accgccttgt ccttctccac cgaggcatgt
     7381 gttgtgcctt ttcatcccgt gaaggaatca cactcgccat ctggcggccg gtcatccacc
     7441 atcgccacgg agtcccgatg actactcccg gtcctgagaa agatacctat ctgaccgttg
     7501 ccaacattct gggagaggaa acggaactgc ttcacggccc cttctcggac gaccaccctc
     7561 tgaaggtcct ggaacgggca gctcctacgg aagcgtcgga caagcggtat ctcgacgaac
     7621 tcaaccgcca catccacgcc tgccatccga agcgtgccgg cctctgcctg tccggcggag
     7681 gtattcgaag cgcaaccttc ggactgggcg tcctgcaatc cctggcacgc ctgcagttgt
     7741 tggacaaatt cgactttctc tctacggtgt ccggcggcgg ctatatcggc agctggctta
     7801 ccgcctggat ccatcgccat ccccggggcc taaaaggcgt catcgaggat ctcaacagac
     7861 catccaaggc cggttcggag gaagcgtctc tgccggtccg gtggctgcga aactatagta
     7921 attacctcag tccgcacttg ggcttcttct ccgccgactc ctggacattg ctcggcatct
     7981 atttgcgcaa ccttcatctg aactggatgg tcctgcttcc cttgctcatg atgccgctgc
     8041 tcgcccctcg ctggatcatc gctctcgccc agctcaatgc caaagagaca tcaccgtcgc
     8101 ccatttcagc cgcctcgctg ctggtcatcg ggataggcct ggcggtcatg gcgttgatct
     8161 atttgcacct ttgcaggccc acccttcgtg agttccgtcg cgacacctgg tggcaaacgc
     8221 tggagagcca gcgctggttt ctgatcgcct gtctgtgtcc cttgctcatc tccgtcacct
     8281 gtctcaccac cgcatgggcc tggtttcgca atggcggggg tggagccgat caattcacct
     8341 gggctcacgc ggtcttcggc ggggccttga tccacacagt cagctggctc ttttccgccg
     8401 tcgccctcaa tcgattcagg gcattcaccc gctggctctt gttagaaacc ctcgccgtgg
     8461 ccttcaccgg cgcgctgggc ggatggcttc tgcgggcatc cttcatcaaa acctgcggtc
     8521 aatggaacgt cgcctcccat gcggaatggt ttgccacact ggcagtcccc ggggtcctga
     8581 cgatatttct cctcaccgcg acgatttttg tcggcctcgc cagccggttc actgaagaac
     8641 aggaccgcga atggtggggc cgcaccggtt cctgggtgct gatcgcaatg gtcatgtggt
     8701 caggcctctg cgccatcgtg atcttcggac ccggcctgat ccactggatg acgccacagg
     8761 tagctgcctc ggtcggcgga ctgtccggcc ttctgacgtt atttctggga ttcagctctc
     8821 ggacgaccgc tcagcaacaa gaggaacggt caaccgtgac cgcagtcacc gacctggcgg
     8881 tgcgcgccgc cgccccgctg ttcatggtct gcgtactgat cgccctcgcc ctgggcacca
     8941 gctggcaatt ggatctgctc gcgcatcact accacatgca cctgaaccat cttgacggtc
     9001 ccatgcagac cggcttggga atcggaagcg atccgtgggg tcacgatcaa attctgcaca
     9061 atactccgtg gtggctgctg ttccgcttca cggcgaccgt agtggccctc gggctgctga
     9121 tgtccgtctt tatcaacatc aatcgattct cgcttcacgc gatgtatcgc aaccggctca
     9181 ttcgagccta tctcggggcg tcccgccggc aggcagaacg tgaacgaacg tttaatccct
     9241 tcaccggatt cgacaatctc gacaatccgt ccgtaagcga cctgcgtttt gatgatgtcc
     9301 gcctcgctcg gtggttctct gcgcaggagc agaaacaacc gactatgcag gcactgctca
     9361 gttacttaca gttatcaaat ccgacggacg agcaacagga agccttgtgc gcgcaactcc
     9421 cgcaggatct gcgacagact caggcatgca agcgcctcag cgaggccgca caccaactcc
     9481 gtctcggtcc agcgtgcgtc gcgaagctga ccgccttaca gatgccggac actgccgccg
     9541 gtcgcgaggc ttccaagtgg ttcagcgatc tcttcataca caaacttcac gcacaaaatc
     9601 ctggcccgtt ccatgtgatc aacattgccc tcaacctggt caagggcgat aatttggcct
     9661 ggcaacaacg gaaagctcaa tcatttacga ttagtcctct ccacagcggc agttggaacc
     9721 tgggctatcg tcgctctgaa gaatacggag ccaatcgcta catcggccaa cctctgtcgc
     9781 tcggtacggc cttggccatt tcaggggccg ccgccagtcc caacatggga taccattcgt
     9841 cctcggcggt gacgtttctt ctggccctct tcaatatccg cctcggctgg tggctgggga
     9901 accccggccg cgcaggcgcg gacacctacc accaagcctc tccgactctg tcgattcgtc
     9961 cccttctggc tgaagcattc ggcctcactg actcctgcca cccctatgtc tatctctcgg
    10021 acggcggaca tttcgagaac ttggggttgt atgaaatggt cttgcgtcgg tgccattgca
    10081 tcctcgtggt ggatgccggg tgtgatcctc gtctcacctt cgaggacctt ggaaatgcca
    10141 ttcgcaagat tagaatcgat ctcgggatcg acattgaact aaacctggac cagatcaagc
    10201 gggcggaggg cgccacgacg agcaatcgcc accatgccat cggcaccatt cgctatgaca
    10261 aggtggatga caatgccacg gccgggacat tgatctatct caaaccgtca ttgacgggca
    10321 acgaaccatc tgacgtgcag gattacgcgg cccgccattc cgcatttccc cacgagccga
    10381 cctccgatca attcttcgat gaggcccagt tcgagtccta tcgccgtctg ggtgagcata
    10441 tcgcgcaaga ggtcctcagg ccggcgctcc atcgcaccga gcaggacttt tcacgactgt
    10501 gccatgcgtt gcgttcgcac tgggtgacga ccccgccggg aatgcaggaa tctttcctcg
    10561 gcgagacgga tgatctcaaa gacctcgagc ggctcttgcg ggacgatccg gacctgttgc
    10621 gctacgacat ggagatctat cccgagctct gttccgtctt cggactggat tgcggcaacg
    10681 tcgccctcaa tcctcgcgcc gcgctccata cctgcaacct gcagattcag cttatggaac
    10741 aggtctatct cgcggtgaag ctcgaagagt tccacggcca tgcgctcaat cgcggatgga
    10801 tgaacctttt tcggcgttgg acttcctcgg cgacgttccg aatgttctgg cccaccgtgt
    10861 gcagcatgta cagccagcca ttcgttcgct ttgcggggca gcatttcaat ctttcaatcg
    10921 accaggtcgc ggtgctgcag ccactggagc agaccggtga ccttagctcg ctgttccggg
    10981 agttattgat cgaatgggcc tcggtacccg attatgcgga gacattcatg aatgccctga
    11041 agcggccgct gccgctggag gaagtcaccg atggcccgcc accgccgctg gcggcctggc
    11101 agatgcgttt gccggccatc gactcccagg ccggaccagt cggttccggt gaaattctgg
    11161 ccgtcgtcgc cgtgactcgg ctttccgtga ccggacaccg gctcgcctta tatggttggg
    11221 ttcgtcctgc gtaccgggga ttgggtctgg ggcatcgtct gttcgggaag gccattgatg
    11281 tgctgacgga ggtatattcc ggccataatc tgatcgtcga tttaggcccg gacaatgccg
    11341 cctgggccgg cacgtccata cgcaatgtcg gatggctccg gttctatgaa cagttgggat
    11401 ttacacggga tcgttcggat cccaagcgct ttcagatgat gcgggagctt tcgtaggacc
    11461 gggaaaagaa ccagggcagg taccgttcag accgagggga gttgcacgga cgagagcagc
    11521 gtctgaacct gtcgaagtag caattcacga ctgaacggtt tttccaacac gccatgggct
    11581 ccgaacgcca tcgcaatctt gagaatgtcg catccttgca ggggctgacc ggatatgccc
    11641 aggattttta cagacgattg gacattgtgt agcatcagga tggtttccag cccatcctgc
    11701 accggcaaat acatatccag aatcatcaga tcaggcgcgt ctcgctcaat ggtcgccatc
    11761 gcctcccggc cgtctgatgc ctggatgaca cgataacctt ccgcctcgag gacagcccgt
    11821 aaccacccgc aaatctgctg gttgtcatcc gcgatcaaaa tcaacggcat tcagggccct
    11881 ccttgctgtc acacgtagtg gccagaagac atggaccggc ttggcagagc tctcgcgtac
    11941 agtgtcgcgg cctgcgttcg cctgctgatt cccagtttgg aaaaaatatt cgcgaggtag
    12001 tttttgacgg ttttatcgga gagcgcgagg gcggaagcga tttccttgtt cgttttgcct
    12061 tcggcgacca ggggcaacaa ccggtattcc tgcggagaaa gggcagccaa cgacttggag
    12121 gaaaggccat ttcccatgtc gtgcacttcc ctcagcacca taccgagcaa gtgcggttgg
    12181 aacaacacat caccggacgc cactctgcga acggcatcca gccaggtgtc aacgggcgca
    12241 tctttgagca gatagcctct cgctccggcg acgatggcct cctgaaccac gacgggatcg
    12301 tcctgaatac tcagcatgag cacccgctgt gcgggattgc gagcgaccat cgtccggcaa
    12361 gcctctacgc ctgagagatc cggcagacgg ctatccagta acaccaggtc aggcttgacc
    12421 tgttccacca gctccatccc ttccacagcc gacatcgctt ctcccaccac gcgaaaatcc
    12481 gtcgctgtca gaagttgttc ccgaatcccg agacgcacca tccggctatc atcgatcaat
    12541 acaatgcgat agacagactg ttgctccatg tctcccccat tgcgataaca ctgaggcctc
    12601 gccggggtac tagtttcggt acattcgcgt agtaggaata taggcaggag gtggatttac
    12661 gcgtcagcat tcggctgacg cgaccacaac ggacaggcga ggctctaagg agaaagacta
    12721 agacctaatc aatcaatcgc gcttcgatgg catatcggat cagttcaccg gtactttcgc
    12781 acgacagttt ttccaggatt ctcgcacgat acgtactaat ggttttcacg ctgaggcaca
    12841 gttcttcccc gatcatcgaa accgatttgc cctgtcccag caaccggagg acttgcagct
    12901 cacgatcgga gagccgcgca tggagttccc cctccgtccc ggtttcaaag gcctgcgcca
    12961 gctgttcggc aagagcggcg gtaatatacc ggccgccttg caacacccgg ctgaccgccg
    13021 ccagcagctc ggagggcgcg ctttgcttgg tgagataccc ggaggcgccg gccctgattg
    13081 cccgcattgc gaattctttt tccggataga ggctcaacat cagcacgggc aagcggggtt
    13141 ggagcaactt ggcctccttc aagacatcca gcccgtgttt atcgggcagc gcgatgtcga
    13201 gaatcatcag atcccaaggc tgacgccgta cggcgtcgag cgcctcctct ccggtttcag
    13261 cctccaaaat ttctgagcac agctgctcgt cctcgagtaa atcacgcaca cctcgacgga
    13321 cgaccggatg gtcatcggct atcagacatc gacatttatt catcaactgt tttcctccgc
    13381 cgccgacgtg atgccgtcgt ttcatgttgt gtttcacacg ctggctccgg ctccgaactc
    13441 accggaatca ccacatgcac ccgtgttccc gtctccgaac tggaaaggat gtccacctgt
    13501 ccgcctaata tttccaccct ttcccgtata cctcgcaagc cgaatccccc ggtgggagcc
    13561 agtcccgcca catcgaaacc gatgccgtca tcctggaccg tcatctgcca attctgttgg
    13621 ccgctcgtaa tggcgatggc cacagtcgtc gcgcgcgcat ggcgtatgac attggtcagc
    13681 aattcttgca caatgcggta gaacgcggtt tccagcgtgg gacagcgtga cggtcccccc
    13741 tccccttgct cgaacaccag cgaacagcgc agccgggtgc gcgcctgcac gtcggcaatc
    13801 agggcctcca atgcggcctt cagtcctaac tcttccaaca ccggtggccg gagggcccgt
    13861 acgatctgac gcaggcggac aaagagcaga tccgtagttt ctaacgcgcg ggccaatcgt
    13921 tcctggcttc gagggcccaa ggctttggaa cgcgaggcga cactccgctt gacggaggcc
    13981 agatcgaact tgagtgaggt caacaactgc cccagttcgt catgcaactc acgagagaga
    14041 cggcgacgtt cattggattc gaccatttgc agttcctgcg acatggcctg aaaacgtcga
    14101 taggcttcct cgagcgatgc ataggcgcgc ttgggacctg tcacatcgat catggcgccg
    14161 atcatgcgta tggccattcc cacctcattt cgcacgatgt gggcccgatc cagaaaatag
    14221 ccataggtcc cgtcggccag caggaaccgg tactctgcgg cgagcgtctg cacctccgac
    14281 tcaatggcct gttggaccat ggccagaatc ggtgccttat cgtcgggatg taaccgcacc
    14341 gtccaggcat caatgctcgg ttcccgctgg ggatcgtacc cgaacttctc ccgggcattg
    14401 gggctccacc aatgcgcgtt cgtcacaaga tcccagtccc ataacacgtc gttggtggcc
    14461 tgagccacga gacgaaaccg ttcctcgctg gatcgaagcc gaagctccgt gcgctgccgc
    14521 tccgacatgt cccgaaccgt ggcgaagtta tactcgcgtc cttcgaagac cacgaaattc
    14581 gccacgacct ccacgggata gatctcgccc gatttggatc gatgttgcgt ttccaggcgc
    14641 tgacggccgg tctctcgaag ctcctgccag tggcggaccc aggcttccat ctgataattg
    14701 ggatcgatat ccggcacggc catgtgcagt aattcgtctt tcgtgtagcc cgtccgttcg
    14761 catgccgcgg cattcacgag caacaggcgc gctgcctcgt ccacccaaaa caccatgtca
    14821 gccgcgcgtt ccatcgcgaa ttgcgtcaga tgcaactgcg cctccgcccg cttccgattc
    14881 gtgatatccc gcatgaacgc gcaataatat tcccggcctt cgtggaccag aaaattgatg
    14941 gtggtgtcgg tctggatcac cgttccatct tttttctgat gccgggattc gtacgagaag
    15001 gacttcctga ctttcaaatg ctgccagcgg gctcgccagt tttcgcgggt gaaattcgtg
    15061 tcgatatcat gcacggtctt ggataaaagt tcctcacggg tatagcccaa catggcacag
    15121 gctgcttcgt tgacatccag aatccgtgcc tccgcactga ccagaaaaac cgcgtccacc
    15181 gcctgctgaa tggagaactg catgaggcgc agggcgctct ccgttcgctt ccgttcgatc
    15241 gcgaggcagg ccagatggga ggcccgggcg acaatcggcg cttcagcggt cggctgacgg
    15301 ggtgtctgat tgtagatggc aaaggtgccg aggatttttc ctgctgccga gatgatgggg
    15361 gtcgaccaac aggctcgaag cccatgcgcc agggcgagct cacgaaagtc cgcccagaag
    15421 ggatggctcg caatatccgt caccaccact tgctgtccgg tgaatgcagc cgtgccgcat
    15481 gaaccgcgcg caggaccaat ggccatcccg tccaaaattc gagcatagga cgcaggaagg
    15541 ctgggaccgg cgccatggcg caagtgggct tcatcctcct ccagcagcaa cacggagcag
    15601 aggacgccgc tggacagtcc ctcgatcatc aggcaaattt ctgccagcac tgccgacagt
    15661 ggagcatccg atcccatcat ctccaagatg cgggtttccg acgcgagcat ctgctccacc
    15721 ttcttgcgct cggtgatgtc tcgcccgaca ccggtgacac cgatcacccg cccttgcaaa
    15781 tcccgatagg gagcaccggt ccagtgatac agcatggtcc ggccatcttt agccaacaga
    15841 tggccctcca actcggcgta gcccttctcg taggcttcgc ggatcgccgc ggctgttttt
    15901 tccgcttcat cggaaggcac catttcaagc gccgacttac cctgcaactc ctccctcgta
    15961 tagcccgtca ccgtttctaa tttccggttc catttggcca ctcgcccctc gagatccaac
    16021 gcaaaaatga tgtcagggac ggtttccatg atcgtttcaa gatgactgag cgcctcatcg
    16081 cgttcacgct cagactgctt gcgctcgctg atatcggtaa tcgtcccgac catgcgcaac
    16141 gggcggccat cctccgctct ggcaacgact ttgccgcggt tcagatacca tcgaaactca
    16201 ccggatttct gtcgcagccg gacttcgatc tccttgaggg gaatgcggcc gtccacgtgt
    16261 gcctgcaaca cgtccatcat gcgggcgaca tcatcgggat gcagaagggt cataaaaaat
    16321 gcccgggtgg gcaggacttc ttcggactga tagccaagca atcgaatcca ctgcgggctg
    16381 tagtacacct catccgtccg catgtcccag tcccacatgc catcgttggt ggcctcaatg
    16441 gcaaagtgga accgctcttc actggctcgt agcgcgctct cggctgattt gcgttcggtg
    16501 atatcccgca caaacgcgaa atggcattcg accccgttgt aatggacaaa gttcacattc
    16561 acatcgatgg gaatcaggcg gccatctttg gcacgatggc aactctcaaa cgagacgcgg
    16621 ccccgttctt tggtgtgtgc ccaaaaggtt ggccatgttt cagcctggaa gttcgggttg
    16681 atgtcgtgga ctctcatccg ccgaaattcg tctttcgagt agcccagcat ggaacaggcc
    16741 gaatcgttca catccagtaa ttccgcgtcg gcgccgaccc aataaatggc ctcggtcgcg
    16801 ctatcaacgg tgtatttggc cagctggagc gcttcctggg cgtgcttccg ttcggtgatg
    16861 tcctgtacca ttgcgagggt atagagcggg gtgccctcct cgtcgcgtat gacggataca
    16921 cccacctggg cccacacgac gcctccgtcc ttcctgacgt atcgcttttc aaacacatca
    16981 tagacccgcc ttccggataa gagcgtttga atacgatcga gattctgccc gaggtcgtcc
    17041 gggtgggtaa tcgattgaaa tgtgttggcc agcagttcct gctctgaata gccgagaatt
    17101 ttacagagag cctggttgac ctcctggatg cgacctccca cgtctgcgat ggacatcccc
    17161 actgtcgcat tgcggtaggc cgcacgaaat cgttcttcac tcgcgcgtct cgcctgttcc
    17221 gcctgcttcg cttccgtgat gtccttcacg tacgcgacgc gtgacaccgg cactccatcg
    17281 gcatccctga tgagcatgac ctcgaccatc accgggaaaa gacttccatc cttgcgccgg
    17341 tgaatggatt cgaatgtcag gccgcccgtg tcatgaatgc gctggattcg ctcttccagg
    17401 gcggcatgtt cctctggggg atagatgaaa aaaattgagc gaccgatcaa ttcctccacc
    17461 gtgtacccgc attgccgggc gaacgccggg ttcacggcca gaaaggtatt ggttgccaaa
    17521 tccgcgtggg caagaccgag ctgtgcctga tcaaacacct gttgccagcg tcgcgcgacg
    17581 tcttcagcct gcttgcgctc cgtaatatcg gttgacgccc cgaccatacg ataggcgcgg
    17641 ccctggtcat cccataaggc ctgggcccgt gtctgaaccc atcggtacgt gccgtccttg
    17701 tgtaacagcc ggcattgcac gtcatacggg gtccggcggg cgaaatgtct ctcaatctcc
    17761 gctttcaccc gcggcacgtc ttcgggatgt acgcgctgaa aaaacgagga ttcgtgatcc
    17821 ggcaactcgt cttcggcaaa ccccagcagt gccttccagc gcggagacag aacggcctga
    17881 ccggcaagaa cattccaatc ccagatgcca tcagaagtcc ctcgaagcgc cagggcatag
    17941 cgctcctcgc tctccttgag ctgttcctct gcaagatggc gacccagttc actccccgca
    18001 cgaccggcgg ccacacgcag caaggcttcc gcccgttcag catccttcag gggggatcga
    18061 ccgagcagcg cgatccagcc aactggccct ccagtcggcg agggcagtgt cagagccatg
    18121 tacgattcga tggacaggtg gaccaacacc tcgtcctgtg ggaacaaggc ctggacgccg
    18181 gaagagtagt ggcagaatcc ttcatggagg acatgctcgc agggggtccc ggcgagggca
    18241 tagtccacag gatccagccg gccgttcggc ccccacccgg ccacggaatg cgcgcatcgc
    18301 ccttcgggca gtgcgcgaag acagaaaccg tagtccgcgc caatcgtttt taccagggtc
    18361 tccaccaggt cctgaaggaa cgccgtgccc gtgagcctgg tggtgggggc catcaggcgc
    18421 agcacctcct cattccgttt ctgaatagtg agatctcgca ccgcgacgat gccgccgctc
    18481 cagtctccac cagaccagca cggcacatgg ttccagcgca cctgcagcag agagccatca
    18541 ccgttcacga acacatcgtc ctgttcgagc acggcggtcc cgctaaggca ggcatgcatc
    18601 aacgtgcacg atccgtcctg cccctcttca tccccatcgc caggacggtg cacacactca
    18661 tgcaacaacc gccccctgag gtcagaggct aaaacgccta gaagacgctc tgccccccga
    18721 ttagcccgaa gaatccggcc gctccgatcg atcacgaagg ccgcctctgt cagggtctcc
    18781 acctgatcat ccgtggacag cgagtcgacg gatgatcgtt cggacctcag catgcgcagt
    18841 tccaggagtg ccgcagtctg ccgcgccaat cgtcgaatgg tcaggaggtc gtcctccttc
    18901 accgtccgcg gaatcgaatc cattacggca atcgtgccga ccgcataccc gtctcgagtg
    18961 acgagaggcg cgccgcaata aaatctgaca tgcggatgtc cgcggaccag cgcatgggat
    19021 gaaaagcgat catccgccga ggcatcctcg atgaccatcg gatctttccc ttgaatggta
    19081 aaagagcaca gcccagacgg acggtcggtt tggctggtgt ccagacctac agcggccttc
    19141 acccactggc ggtcgtgatc gatgaacgtg atggcggcaa tcggagcctt atagatcgag
    19201 gcggccagtt gcaccatctc gtcgaagtcg gcttctggag gagaatcgag gacgcggtag
    19261 cggcacaggg cccctacacg cgcggtttca tcgggaggca gcgtatgcgt ccccatgcgt
    19321 gagctcccac gcgcgagaga atgaaatggc tgtattcata catccggccg agtcatatca
    19381 caaataccca agatggacta gggccatctg gaacgacact cttcctgtag aggcgaccgc
    19441 gcgagccacc tctgtggact ggctacaacc aagccggggc agaggtgccg tcggcgccgc
    19501 gccaggcggg cacccacgcc agtcccacca gtttgacgat gatatttgac ttggtgggct
    19561 ttagtgaaat ctggtcgagc ttttcagtca agggatccgt cgccgccatg agcgcatcgc
    19621 tttccgattt gaactgggcc tccaggtccg tcagctgttg ctgaagcgtg gccacgtttt
    19681 cttccgcatg gccgacctct tgcgattcct tcatcacacg ccccgcgctg cggatcgccg
    19741 ttgtcgcacg gccgatgttc gtcgcgctga gtgtcttgcg gcccagaaat gcacccagta
    19801 tcgatgcgcc gacagaaatg accgcctgca cctgactgga tcgagattcg ttctgttgtt
    19861 tctcccgggc ctgctcggcg cgccggaccc ggtctcgaag cgtcgtaatt ttgggcgcat
    19921 attttttccg aagctcgtcg ctcagcctgt cacgctcttc ccggccaacc tgctgtaacc
    19981 ggacgcgaaa atcgcgttcc gactcgccgg gcttcgatag cgtattcgtg gtcggacttc
    20041 gccagagttc gagcttttgc gtacgaaaca accagccgga gaggtcgctc ttccagctgt
    20101 cgtattgctt ccctttgctc gctgcaccgg gcagagccgc gaactccgca tcggcggccg
    20161 gttcgcgttc aaggtccgcc acttgcacat ccgcagccgt cgactgctcc cactccaccg
    20221 ggacgacgcc actgctgata gcagccagtc gtgtgagatc agtgctggcc tcgatccctg
    20281 ccttgttgtc ggtaaatcgc acctgcgccg cacccaagac catcggttga tagatgagcg
    20341 ttgctccact tggttgtgtt ccccggagcg gaagaaaata ttgcggcaca tcgggaggca
    20401 acataggccg ggaggccgtc gtgcgcgacg ccgcactcgt cgagccggaa gcccctgcgg
    20461 ctcccagttc gtcctgccgt gaggtccgcg ctggcagctg acgcttcacc ggatccatca
    20521 actgtttgat ctgtgtgcgt gtcagtggcc cccggagata tgacagcgtc cagcgtgttt
    20581 gaaagacttc cggcgcatct tcatgcacgt tattcaacag aaagacccga ttgcccagac
    20641 cggcaaggat ctgttccatc tgctggcggt caaacttctt tccgctgctg gccgcggccc
    20701 cttccaagcc ttcgatcacc cgcgccttat cgcgctccgt ctgcaaacga ccgatgaacc
    20761 aggtgccggt attggccagc cccttataat cgagatccac cggattttgc gtggccaaca
    20821 cgacacccaa cccgaacgcg cgcgcctgct tcaacagcgt caacagaggc gctttggagg
    20881 gagggttcgc gaccggcgga aagtagccaa aaatttcgtc catgtagagg atcgcgcgca
    20941 ggctggtggt gccggactgc gagcgcaccc accccagtgt ctggttgagc agcagtgcga
    21001 caaaaaacat ccgctcggcg tcgttcaaat gggcaattga aaaaatcgcg atgcgcggct
    21061 tcccggacgg cccgtacaaa atctgtccga catccagcgc ctcaccttcc atccaggcgg
    21121 caaaccccgg cgcggccagc agattgttca attgcatggc cagggcgaat cgttctttcg
    21181 aagggaagaa tgattccaca tccagtaccc cgactttggt catgggaggc gtttg
//
LOCUS       DAOQDS010000062        21188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_942_length_21188_cov_23.219751, whole genome shotgun sequence.
ACCESSION   DAOQDS010000062 DAOQDS010000000
VERSION     DAOQDS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 21188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21188
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_942_length_21188_cov_23.219751"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1156..2544)
                     /locus_tag="PK589_14215"
     CDS             complement(1156..2544)
                     /locus_tag="PK589_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNJ20812.1"
                     /translation="MLFSRRFRIALVLSLVLLPVSLSLSVTPVQAQTPRLQGQSASAA
                     AMGNAFVAQADDPSALHYNPAGMTQLHGFQTLFGTSLIGGTTQFTGPSGAQATGDRNG
                     SLAYPPPGHVYLVANLKDLGFAALGDFSAGIGLNNPFGSLTRYPNDGPFRSALTFTTL
                     PLLDIKPTIAYKLNDQLSFGLGADIYTFSGLFGEGHLEQKSVWPGGLGIPAGSLVEIN
                     GSDTTAGFNVSLLYTPYRNSEGKPLVNIGMVYRSQATFHLTGNFLANGTSIAGTAATF
                     VLPQVFSGGIAVWPVRTPDREWKLEFDVDYVGWKSNRTLDIHLTNGTVLPFPQNWQSG
                     YTAMVGTEYRWLRLASLPDWDVALRAGYMNQQTQVPDRTFNPGVPSANTHIPSVGIGF
                     ACHEHGYFLGLIRCGELGIGPLKPKLFAIDLSYQAAFYEVRTVTGNQNPTVNGRYDTL
                     IHAGSLSLRFNY"
     gene            complement(2549..5266)
                     /locus_tag="PK589_14220"
     CDS             complement(2549..5266)
                     /locus_tag="PK589_14220"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ20813.1"
                     /translation="MTDLLSSDHSILERPFMEFRPFGLNDKGEKISDISGVVVQSNVD
                     YMCDYLSRTAGSQAAAAAVDELCRLLNARLPDRTYHVTPDFLRNIWNSYSYEFVCYLR
                     EFCEQLSGDPQFHINVGTDRKVPPLIQILCRPFSTQQLYKMWPYFGRKYVRDVLEFEV
                     GRVTRRSAVLRMTFTERALVQFGPYRKRCVDVICLSCKSSIARVQTQLHGTPAATVKD
                     LSCTAHGDAYCEWEFTWTPQVRSSIPVAMWTLVAGGTFTYLHVRWPDVPTFEALGLAA
                     VPASLLWWMITSHMRQATKPLQRLIKDQEQVVDARHEELREAYLEQQSTAVMLRRKVN
                     DLTTLHRAGLLFSSTFDREELLTDVLDTIVRDLHYDRAMITQFDRTRQVSHDFRVRGM
                     PQEVADFLRTQEVSVTDPETVEGAVFLRGEPVLTNNVHEIWDRLHPFDQKLIAMVNVK
                     SFITVPLKTHDAVIGSLSVDRTHNQALTQDDLDMLVTLASQVAIALDNAQAYREIEAL
                     NAGLEVRVRERTAELEAANTQLKQMDRLKSKFLAHVSHELRTPLTSIVGFADNMLEGL
                     VGSLNMKQEQYLTRIRANGTRLARMITDLLDLSRVEAGKLVLSFDHVALQTVVSDVIE
                     QLLPLAIAKHLQIELRSPDPALLVWADPDRVSQILTNLLDNAIKYTPDGGHITVDLSV
                     GDHEMARVVVRDTGQGIPADALPKLFDPFFRVHHQERSQTKGLGLGLAIVKDLVDLHG
                     GSIMVHSTVDEGTEFTFTLPLDSRAPEPAAQVPAARRRLLVVDDDADICDLLRDRLES
                     EGFHVDTAADGRTALRILAETPVDGVLLDIALPEVDGFEVLRQLRPQQPALPVVMMTA
                     VEALDRAMAAVEAGAQGYLLKPFDAARLRQIVDHWFLAGASKTDPTSGR"
     gene            complement(5335..7113)
                     /locus_tag="PK589_14225"
     CDS             complement(5335..7113)
                     /locus_tag="PK589_14225"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HNJ20814.1"
                     /translation="MRTRLYRSFALTALLLTLSPLHCRAIDPSPNQISAESLNQSLSD
                     QDLQQWYHLSAGTQLIPYDWLVALLDESLAKTFAVTGLVEDPRHPDKLPVGFAKTEGP
                     NVPVPLVGFTCSFCHTTQFRYKGHSIRIEGGPSLQNNAALVNALIQRLGALTPPDVSA
                     FLNSLKAEAPPDPFKTFANRVLASRGEAVTAYTLTKLAQDVRVLTQALVSRSGKDISP
                     QQWGPGRFDALGRGGNTVFAPLNPDNLRPANAPVSIPALWGVWEFDWVQWAGSIQHPL
                     ARNIGQVIGVNAGLFSWARPGVPTPSDKNKVFHSSIDVTSLTILENLARRLPPPQWPS
                     VFPPINRELAARGKDLYHGNKAKGIPNLCAHCHVPSKMSHSSANSPSLQITMVPLQEI
                     GTDSLYLENFSQRTVDTSFLGRGRISAREASEYVTTELMTLDKTGDLSSYRDRPNIWR
                     DKAQYIARPHFGIWATAPYLHNGSIPTLYELLSPARERRACFVLDPNMEFDPEKVGFV
                     TGDCANPSQSPTLPPRLEFRTALPGNSNRGHEFANTPDCDSAKSNGVLGCELPVNDRL
                     AVIEYLKTCDLDRLILPNAPACPNFD"
     gene            complement(7434..7949)
                     /locus_tag="PK589_14230"
     CDS             complement(7434..7949)
                     /locus_tag="PK589_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20815.1"
                     /translation="MDSVQSIFLDLNQLIPIVNHWFHLLSAVIWIGGLAFIVMAVKPG
                     LAEAALPKEYVRPITDAFYKHYKRVAGVLLVVLLFTGGINLHYVNQIFVSQTGQGMQH
                     HPKYLWIFCIKLILVLCLLTLFLYTVIFKTDQDEEEEGENYESIPFQRAALWMGFFII
                     LCAAAMKHLHQ"
     gene            complement(8016..9419)
                     /locus_tag="PK589_14235"
     CDS             complement(8016..9419)
                     /locus_tag="PK589_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ20816.1"
                     /translation="MGKSLPVLNIPFLGDKLESLQQQISKFISPTSPGTAPNDGRTVD
                     DFKPYLVALNLTKRCNLKCDHCYLDATTKAGGGSDELSTEECFRLIDQIAEVNKGCLL
                     VITGGEPLVRPDILDIARHAVGLGFIVVFGTNGMLINDRMAKTLVEIGVMGVGISIDS
                     LNPEKHDHFRGVPGAFASALAGIEACKRNGLQFQVHFSAQPMNYQELPAVVEWAHTLG
                     ARVLNVFFMVCTGRGEELTDITPQQYEEVLGYLVDCQDQYKDMLVRARCAPHFKRLAY
                     EKDPNSPLTKATGYMGGGCLAGTNYARVTPNGELTPCPYMPLSAGNVRETSFADLWER
                     SDVFNSFRYPQLKGKCGDCEYTDICGGCRARPYVDHGDWLDEDQWCLYTPKGGDKIKV
                     AFNTPEESEVAWDEASELRLSRIPYFLRAMVKKGVERHARENGIALVTIELMEELRKK
                     RFGNDAPVFNFDMRGKD"
     gene            complement(9419..11527)
                     /locus_tag="PK589_14240"
     CDS             complement(9419..11527)
                     /locus_tag="PK589_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ20817.1"
                     /translation="MYKTIYIPVDNSDHSNTAVDVGVELAKTYGSKIVGSHVYAAKMH
                     DKRFKQMEAGLPEEYHDEKELDRQRQIHDSLITRGLQIITDSYLDYVDKKCNEANLPV
                     ERRSLEGRNWKVLAEDINTNAYDLVIMGALGVGAVKDSVIGSNTERVLRRVRNSDMLI
                     IKQPTPVGTGKIVVAVDGSPYSFGGLMTGLALGKAFNVPVEVISAFDPYFHYAAFHSI
                     SGVLNEEAGKVFRFKEQEKLHEEVIDSGLAKIYQSHLDISREIAQAEQTDVKTTLLDG
                     KAFEKIIQYVRKENPWLLIVGRIGVHSDEDMDIGSNTENLLRSASCNILVSNRKYVPP
                     IDTQAEYTIAWTEEALRRMERIPVFARGVAKTAIHRYAIEKGHTIISNTVVDSAVGHI
                     LPKGAMDAMRALGGNLDAAGIDRDKMQADDSVAQDLMGSTLSGMMTQVVEEKPAHSPG
                     TQAYLDRMSQNYFVCDGCGYIGKGDTPVKCPVCSAEGDRFKQVDKSIFEAAAKAEGGL
                     ETDVAYDDIPMQWTKDAKEAIRAVPAGFQRRRAKAKIEKSARKLGMTTITLEYANPMI
                     QEAASEDYTPIFANKGTGTAGQPSAEVPTANGTESATANGNGTEQPQASQAPSSPYTW
                     TPDAQGRLDRAPEGFMRECTKALIEKHADKIGTTVITLEVATEGIEQAKGYMADAMKT
                     GNLKDMIANLTGSSKTGANR"
     gene            complement(11693..13336)
                     /locus_tag="PK589_14245"
     CDS             complement(11693..13336)
                     /locus_tag="PK589_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ20818.1"
                     /translation="MNSILYIFLPCKKVYPIGVTYLADFIHRRKPEVRQRILDLSLFS
                     ESQRTQAIRDAALEFKPDLVCFSWRDIQIFSPHEGDASLEHAFNFYFASNPIKRVAAS
                     FAGLKQLYRYYSHIRANLSYPWLIRKEFPKTQIMIGGGAFTAFADQLIEKLPEGTIGI
                     LGEGEDAILKVVNGESLENERYIIREGKQTRKGNQGSPALLDALTVDLPYLTSIFPQH
                     ASYMDESIGVQTKRGCPYDCAFCLYPYIEGKRVRYRPPEMVVKDISQHYHQWGARRFW
                     FTDAQFITGKEAYPQCTEILERILSEKLEIEWSGYIRTSLITPELAKLMVRSGVGDLE
                     VAITSGSQEVLNNLHMGFKLERLYDGCRYLAEAGFKGKVILNYSLNSPKETEESLLQS
                     VESYKKVASILGEERVFPLMFFLGIQPNTDLEHRLLEEGYLSAGYNPLMLTPTSIRKL
                     LYNPAPLNKIIAKACLTAWEQKEGSRDPRTWTGSLSQTPPAGAKPDQAHYADANLIRG
                     IQNNSGREALLTLEEILRSRRPAQPTAAATEKTAVSPTS"
     gene            complement(13403..14170)
                     /locus_tag="PK589_14250"
     CDS             complement(13403..14170)
                     /locus_tag="PK589_14250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ20819.1"
                     /translation="MVRAEQSPITEPAAKAVSTWSGQAREQAVQRMFTAIAGVYDLNN
                     TLLSFGLHHHWKRLTASYVPTVTNGRALDIGAGTADLALLIEPKMGEQGHVVASDLNH
                     AMLAEGLRKVSGRGLLDRITCLQANAEQLGFPDNTFHAVTTGFCMRNVGNLTQAFTEI
                     RRVLRPGGRFVCLEFSRPAYGWLRALYDWYSFRLLPWIGTRVARDRTGVYEYLPASIR
                     TFPDQERLATLLREAGFREVEYRNLTGGIVAIHVATK"
     gene            complement(14264..15043)
                     /locus_tag="PK589_14255"
     CDS             complement(14264..15043)
                     /locus_tag="PK589_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ20820.1"
                     /translation="MQSKVLFNKDDHQWVVIGRDPEKDDHVIDTNEYVIIHRGHAMLL
                     DPGGIQIFPQVLAELAKYVRMQDIKVIFASHQDPDICSSLAMWLDLNPAIKTYCSWLW
                     TGFVSHFSTGAVITLNPIPDDGMRIKIGEEGPEMEAVPAHYCHSSGNFSLYDPSAGIL
                     FSGDIGGALVPDHHASLLVTDFEQHIQYMRGFHLRWMPSTTALRGWVQRVRAIKPKMI
                     CPQHGSIFEGENVGKLLDWLESLEVGQWKGSAPKSETRAAA"
     gene            complement(15033..>15380)
                     /locus_tag="PK589_14260"
     CDS             complement(15033..>15380)
                     /locus_tag="PK589_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20821.1"
                     /translation="VGGLERENRLYLVHHCQANLTELQERLKEALRCLGETHQALNGL
                     KMIGLTARYMAFCISSEAAGLAEAEANFKNLAAEISRVVDDLDNKTRAMKDAIEHGQE
                     LLGLLLKGHTYAK"
     assembly_gap    15381..15480
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15481..15979)
                     /locus_tag="PK589_14265"
     CDS             complement(<15481..15979)
                     /locus_tag="PK589_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20822.1"
                     /translation="MHDVLSLFKRNINQLFGITVDILNVGRSLQQLSLSMQILANNGV
                     VQAAKIPGGKGRPMLALVEILNNTPKEIRPEVEALEHLCAGLAKVTASSSNIVWRYHQ
                     LIASLLSAMTQGELSSKSKSLETLSHLRFTTAADVTQLTQHPAFTAVGGLERENRLYL
                     VHHCQA"
     gene            16182..18689
                     /gene="uvrA"
                     /locus_tag="PK589_14270"
     CDS             16182..18689
                     /gene="uvrA"
                     /locus_tag="PK589_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNJ20823.1"
                     /translation="MSNSIIIKGAREHNLKNLDVEIPRDKLVVMTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLEQMGKPDVDSIEGLSPAISIEQKSTSHNPRSTVGTV
                     TEIYDYLRLLYARVGHPFCFQCGQEITAQTVQQMVDAICELPEGSKFQILSPIVRGRK
                     GEYRKELLEMRKAGYVRARINGEMVDLGEDITLDKQKKHTIEIVVDRLVMKPGDALMR
                     RIADSVETSVKLAGGLVGVLSETGKLHLYSDKLACIKCGVSYPEITPRVFSFNSPHGA
                     CPACDGIGYAMTPGATEEDDFTLLERCETCQGARLKPESLAVKIAKKSIAEITGLSVR
                     AAADFFATLKFTERELVIAHRILKEIRERLGFLVNVGLDYLTLDRPAATLSGGEGQRI
                     RLATQIGSGLVGVLYILDEPSIGLHQRDNRRLLQTLLRLRDLGNTVIVVEHDAETMEA
                     ADYILDLGPGAGTHGGRIIAQGTPKQVKANPDSLTGLYLRGAQMVSLPQRRRTPKGFV
                     TVVGAKKHNLKGVNVNVPLGLLTCVTGVSGSGKSTLVLEVLFHSLSQLLYHKKPKIDG
                     CKEIKGVDALDKIIDIDQSPIGRTPRSNPATYTGLFTFIRDLFSNLPESRVRGYKPGR
                     YSFNVKGGRCEACQGDGLIKIEMHFLPDVYVTCEVCKGQRYNRETLDIQYKGRSIADV
                     LNMTVDDALEFFENIPFIKSKLQTLHDVGLHYVKLGQSATTLSGGEAQRVKLSRELSK
                     RPTGRTMYILDEPTTGLHFADIQRLLDVLDRLVETGNTVLVIEHNLDMIRNADWIIDL
                     GPEGGDRGGEIVAEGPPKEIAKVKRSYTGQVLREAGV"
     gene            19196..19753
                     /locus_tag="PK589_14275"
     CDS             19196..19753
                     /locus_tag="PK589_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20824.1"
                     /translation="MPLLIRILIVGFLGLLNGCYVGEGTGYLRFEGKVSNEEGQPLDG
                     REVRIQLSPVESIHVRNVGKVAHTQNDGKFVIETHGIVGVVYVAPIGWIVVPLGVVIA
                     ALTPWTNSEVQDFLRPSYTFGPDCLDHPKIIALKIDSADYGMTFIEKDIVRKLYHGVE
                     RKNGESCARVYDFGELEISMGSLSR"
     gene            19815..20144
                     /locus_tag="PK589_14280"
     CDS             19815..20144
                     /locus_tag="PK589_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNJ20825.1"
                     /translation="MHTILLLTISNIFMTFAWYGHLKYKESPLWIAILASWGIAFVEY
                     CFQVPANRIGHYEFTAAQLKTIQEVITLMVFCVFSAVYLKEELKWNYLVGFGMMIGAV
                     FVVFKEW"
     gene            20377..20805
                     /locus_tag="PK589_14285"
     CDS             20377..20805
                     /locus_tag="PK589_14285"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HNJ20826.1"
                     /translation="MKSSSSIHNLACALCVAAAGLMVGACNTTKATVDTTVNFFSSTS
                     PNDLFTSDGMVVKEQKINLFAGVAYENLRQEAATGSGQYVTALAALYEIPVSKQQEFG
                     QVLQRNHADLFAADLQDDHSAHLKMVSALNRELTAAALLP"
     gene            20972..>21188
                     /locus_tag="PK589_14290"
     CDS             20972..>21188
                     /locus_tag="PK589_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20827.1"
                     /translation="MRRQHIFAVAAVLALTGLPAFAADLEVQVVDKNCFIEIFEDDNF
                     DVDDPHVVLQGPKEYATLKNLAGKDWSN"
BASE COUNT         4492 a         5677 c         6287 g         4632 t
ORIGIN      
        1 ggcacgccga attcgccgaa gctatgaaag aggttcgtcc caccaccggg acgagtgccg
       61 cccgtgatat taaaagaatt tccgcttgtg gtgacggtgg tattcaggcc cgaggaggtg
      121 atcggtgagg atggttgagc ctggcacaac cccggcatga acacttgtgc tagcacacaa
      181 gcaagcaaga cagcgaggtg tgggagagct acaggcctgg cgcgtaatcc tctcgtcacc
      241 atagaagccc ttgtcccccc ccacaccgac atggggctcg gccggaatct ccaacagatt
      301 actgggtggt tgcagttggt tgagccccag actccgttga gtcagccttc tgttcaaacc
      361 ctgtgctgtg tttcggccat gcctgagttg ttggctaccg gtgctttggt ctccttgaac
      421 gttccagtgg gtgcggtcat ccagatacga caggctgcga ctagaaggac gaggcccccg
      481 agtaggagaa aaaagagcgc gaccatgatg atccctcctg aaggtgaggt tggctcatca
      541 ctgtcagccg aagagaagtt gtaattggct acgtgacgcg caaggcctgt gccatggacg
      601 gcggttgttt gagcacggat gtgcgggcgg gaagatctgt agacattctc acgacatctc
      661 gcatcaaggc tagcgtgccg atccgctatg cgggcctgcg tcgagttgaa aggtgtatcg
      721 gattggattc gtggcgtatc ggatccgata cgaatgcgcc cgccatgcgt ttgtgcgaag
      781 gcagcgcata gcggatagag tgatgtgcag actcaggaaa ttctgacacg ttcagatcga
      841 tgagagcccc cgctcgggag tgcggggaaa ggagccgcgt ctccgttgag aacgaagacg
      901 cggcagttgg cgtggagatg aaggcggtct actcagcgct ggatgggcac aatccaggag
      961 gcgagaaatc cggtcggtta acgcaaccga cgatcggaga ccggctccac gccacgccgc
     1021 tttggtcgta tggaactggg cacgttgctt cgctcgtgtt gctatcgaga tgtgagtgaa
     1081 gcaatcactg tgcccttgcg tactcgaaag tcggggcgac gagtggcgtg tcgagttgag
     1141 aaatggctga ttggttcagt agttgaatcg caaggagagc gagccggcgt gaatcaatgt
     1201 gtcgtagcga ccgttgacgg tcggattctg attgcccgtg acggtacgta cttcatagaa
     1261 cgcggcctgg tatgacaggt caatggcgaa gagttttggc ttgagtggac cgatgcccaa
     1321 ctcaccgcat cggatcaagc ccaggaaata tccatgctca tgacaggcaa acccaatgcc
     1381 gacggaggga atgtgggtat tggctgatgg aaccccggga ttgaaggttc gatcgggaac
     1441 ttgcgtttgc tgattcatgt agccggcacg cagggcgaca tcccaatccg gcagtgaagc
     1501 cagccgcagc catcgatatt cggttccaac cattgctgtg taaccgctct gccagttttg
     1561 cgggaagggt agcacggtgc cgttcgtcaa atgaatatcg agcgttcggt ttgatttcca
     1621 tccgacatag tccacatcga attccaattt ccattcacga tcaggggtac gtaccggcca
     1681 gacggcgatg ccaccggaaa agacttgcgg caggacaaag gtcgctgccg taccggcgat
     1741 cgaagtgcca ttcgccaaga agttgccggt gagatgaaag gtggcctgac tgcgatagac
     1801 catcccgatg ttgacgaggg gtttgccctc gctgttgcga tagggtgtgt agaggaggct
     1861 gacgttgaag cccgcggtgg tatcactccc gttgatttca acgagtgagc cggcgggaat
     1921 gcctaatcct ccgggccaga ccgatttttg ctcgagatgc ccttcaccga acaggcctga
     1981 gaaggtgtag atgtctgcac ctaatccgaa cgatagttgg tcgttgagct tgtaggcaat
     2041 tgtgggtttg atatcgagta gcggcagtgt cgtgaaggtc agggcgctgc gaaacggtcc
     2101 atcgttggga tatcgggtca acgagccgaa ggggttattt aacccgatac cggcgctgaa
     2161 atctcctaaa gcggcgaagc cgaggtcttt cagattggca accagataca cgtgccctgg
     2221 tggcgggtag gccaggcttc cgttgcgatc tcccgtcgcc tgggcgccgc ttggaccggt
     2281 gaattgagtg gtcccgccga tcagcgaggt cccaaagagg gtttggaatc catgcaactg
     2341 agtcataccg gccggattgt agtgcagtgc agagggatca tctgcttgcg ccacaaatgc
     2401 atttcccata gcagcggcgg atgccgattg cccttgaagg cggggagtct gcgcctgaac
     2461 aggtgtcaca ctgagggaaa gactcacggg aagcagaacc agggacagta ccagggcgat
     2521 gcggaatctg cgagagaaga gcatagggtt atcgtccact tgtggggtcg gtcttgcttg
     2581 cgccggcgag gaaccagtga tcgacgattt gtcgcaagcg ggccgcgtcg aatggtttca
     2641 ggagatatcc ttgtgcgccg gcttctaccg cggccatggc gcggtccaat gcctccacgg
     2701 cggtcatcat caccaccggt aacgcaggtt gctggggccg tagttgccgc aacacctcaa
     2761 atccatccac ttcgggtaag gcaatgtcca gcaagacgcc atcgaccggc gtctccgcca
     2821 ggatgcggag ggccgtccgg ccgtcggctg ccgtgtcgac gtgaaatcct tctgattcca
     2881 gacggtctcg caaaaggtcg caaatgtccg catcatcatc caccaccagc agtctgcggc
     2941 gagcggcggg tacttgcgca gcgggctccg gtgcccgtga gtcgagaggc agagtgaatg
     3001 taaattcagt gccttcgtcc accgtactgt gcaccatgat gctgccgcca tgcagatcca
     3061 ccaaatcttt cacaatcgcc aaaccgaggc ccagcccctt cgtctggctc cgctcttgat
     3121 ggtgcacgcg gaaaaacgga tcgaagagtt taggcagggc gtcggcgggg atgccttgac
     3181 cggtgtcacg gaccacgaca cgggccatct catgatcacc gactgagagg tctacggtaa
     3241 tgtgtccccc gtccggggtg tacttgatag cattatccag gagattcgtc aaaatctggc
     3301 tgacgcgatc cggatccgcc cagacgagca gggccgggtc aggactccgg agttcgatct
     3361 gcaagtgttt ggcgatggcc aacgggagaa gttgctcgat gacgtcactg accacggttt
     3421 gcagtgccac gtggtcgaag gagagcacca gtttgccggc ctccacgcgg gagaggtcga
     3481 gtaaatccgt gatcattcgt gcgagcctgg ttccattggc tctgatccga gtcaggtatt
     3541 gctcctgctt catgttgagc gaaccgacca gtccctcgag catattatcc gcaaacccga
     3601 cgatgctcgt gagcggggtc cgcaattcgt gcgagacgtg ggcgaggaat ttggatttga
     3661 gccgatccat ttgcttcagc tgggtgttag cggcttccaa ttcggcggtt cgttcacgaa
     3721 cccggacttc caggccggcg ttgagggctt cgatctctcg ataggcttgg gcattatcga
     3781 gggcaatggc gacctggctg gccagggtga ccaacatatc aagatcgtcc tgagtcagcg
     3841 cttggttgtg cgtgcgatcg acggagagtg aaccgatcac ggcgtcgtgg gttttgagcg
     3901 ggacggtaat gaaggatttt acattgacca tggcgatcaa tttctgatcg aacgggtgta
     3961 accgatccca gatctcatgc acgttattcg tcagcaccgg ttcgccacgc aagaacacgg
     4021 cgccctccac cgtctcgggg tcggtgaccg acacttcttg cgtgcgtaaa aaatctgcca
     4081 cttcctgagg catgcctcgc actcgaaaat cgtgtgagac ctgacgcgtt cgatcgaatt
     4141 gcgtaatcat ggcgcgatcg tagtgcaaat cgcggacgat cgtatccagg acatcggtca
     4201 gaagttcttc ccgatcgaat gtcgagctga acaataatcc cgcgcggtga agcgtcgtga
     4261 ggtcattcac ttttcgacgt aacatcacgg ccgtactttg ctgctcgagg taggcctcgc
     4321 gcagctcctc gtgtctcgca tcaacgacct gttcttggtc cttaatcagg cgttgaagcg
     4381 gcttggtggc ctgccgcatg tggctggtaa tcatccacca caggagcgag gcgggaacgg
     4441 cggcaaggcc cagcgcttcg aatgtgggaa catcgggcca gcgcacatgg agataggtaa
     4501 acgtgccgcc ggcaaccagc gtccacatgg ccaccgggat ggaactccgg acttgcggtg
     4561 tccaggtaaa ctcccactca cagtacgcat cgccgtgggc cgtgcacgaa agatctttga
     4621 ccgtagcggc cggggtgccg tgcaactggg tttgtacgcg ggcgatactg ctcttgcagg
     4681 agagacaaat gacgtcgaca cagcgttttc gatatggccc aaattggacc aacgctcgtt
     4741 ctgtaaacgt catgcggaga accgccgagc gtctcgtcac ccggcccacc tcgaattcca
     4801 acacatcgcg gacatatttc cggccgaagt agggccacat tttatagagc tgttgggtcg
     4861 aaaacgggcg gcagaggatc tgaatcaaag gtggaacctt gcggtccgtt ccgacattga
     4921 tgtggaactg agggtctccg gatagctgtt cgcagaattc cctcaaataa cagacgaatt
     4981 catacgagta actattccag atgttccgaa ggaaatcggg tgtgacgtgg tacgtgcggt
     5041 cgggaagccg tgcgttcagg aggcgacaga gttcatccac cgctgctgcc gctgcttggg
     5101 aaccggcggt gcgacttaaa tagtcgcaca tataatccac gttcgattgg acgacgacgc
     5161 cgctgatatc agagatcttc tcgcccttgt cgttcaagcc gaaagggcga aactccatga
     5221 acggacgctc gagaatcgag tggtcactgg agagaaggtc ggtcatgcgt cggtggcctc
     5281 cttcgatgca tctccgtcat gtacgagcgg caccggatgg tttcacgtcc tctgctagtc
     5341 gaaattcgga caggccggcg cgttcggtaa aatcaagcgg tcgagatcac aggtcttgag
     5401 gtattcgatg acggccaggc gatcattcac cggcaactca catcccaaaa ctccattgct
     5461 ctttgcgctg tcgcaatccg gcgtgtttgc aaactcgtgc ccacggttgc tgttccccgg
     5521 taatgctgtc cggaattcga ggcgtggggg caaggttggt gattgagagg gattggcaca
     5581 gtctcctgtg acaaagccca ccttttcagg atcaaattcc atgttcggat cgagcacgaa
     5641 gcaagcgcga cgctcccgtg ctggggataa tagttcatag agagtcggga tagagccatt
     5701 atgaaggtag ggggcggtgg cccagatgcc aaagtggggg cgggcgatgt attgggcctt
     5761 gtcccgccag atgttcggcc ggtcgcgata gctgcttagg tcgccggtct tgtccagggt
     5821 catcaattcg gtcgtgacat attccgaggc ttcccgcgcg gaaatgcgtc cgcgtcctaa
     5881 gaagctcgtg tccacggttc gctgagaaaa attctccaga tagagggagt cggtgccgat
     5941 ttcctgtagg ggaaccatcg tgatctgcag acttgggcta ttggccgagg agtgagacat
     6001 ttttgagggg acgtggcaat gggcacacag gttcggaatt cccttagcct tgttgccgtg
     6061 atacaggtct ttgccgcggg ctgctagttc ccgattgata ggagggaaga cagacggcca
     6121 ctgcggcggc ggcaggcggc gtgcgagatt ttcgaggatg gtgagcgaag tcacatcgat
     6181 agatgagtgg aacaccttgt tcttgtctga gggagtggga acgccgggac gtgcccaact
     6241 aaaaagcccg gcattgacgc cgatcacctg tccgatattc ctcgccaacg gatgttgaat
     6301 cgaaccggcc cactgcaccc agtcaaactc ccacactccc cacaatgccg gaatacttac
     6361 gggggcattg gcaggtctga gattatcggg attgagcggt gcaaagacgg tattcccacc
     6421 gcgacccagc gcatcgaatc gacctggccc ccattgctgg ggcgagatgt ctttcccact
     6481 gcgggacacg agcgcctggg taaggacccg cacatcctgg gcgagcttcg ttagcgtata
     6541 agccgtcacg gcttctccgc ggctggcaag cacgcgattg gcaaaggtct taaaggggtc
     6601 aggaggagcc tctgctttca ggctgttgag aaatgcggac acatcgggag gcgtcagggc
     6661 tccgagccgt tggatcaagg cgttcaccaa ggccgcatta ttttggagcg aggggccacc
     6721 ctcgatcctg atggaatgcc ccttgtagcg aaattgggtc gtgtgacaaa acgaacaggt
     6781 gaagccgacc agaggcaccg ggacgttcgg cccttccgtt ttggcaaagc cgaccggaag
     6841 tttgtccgga tgccgaggat cttcgacaag ccccgtcacc gcgaaggttt ttgccagcga
     6901 ctcatcaagt agtgctacca gccaatcgta aggaatgagc tgggtcccgg ccgacagatg
     6961 gtaccactgc tgcaggtcct gatcagacaa gctctgattt aacgactcgg cagagatctg
     7021 attgggagat gggtcgatcg cccggcagtg cagtggagac aatgtcagta gaagtgcggt
     7081 aagagcgaag gagcggtaca ggcgggtgcg catgattcat ctcctcatga aggacgagag
     7141 caatgagtcg ggataggcat agtcactccg atgctcaagt aaccgagacg accgtatgtt
     7201 ccttgtagga gcgagggcgg caatggtgga gcgatgaaac tcgcggccct ctccgtagca
     7261 gaaagcaact cacatgccat aggcgcggac gaagggtacc tggatcgccc tctgattgcc
     7321 aatagatttt gaaagtcgag ttatgaggta tgaacctttg agactattcc ggcccggcgg
     7381 ccgggcagca tgccgacggt cgagattcgt tgtccggagg gtcgaggaaa ggactattgg
     7441 tgcaggtgct tcatggcggc ggcgcagagg atgatgaaga agcccatcca gagggcggcg
     7501 cgttgaaagg gaatcgactc gtagttttcg ccttcctctt cttcgtcctg atccgttttg
     7561 aagatcacgg tatagaggaa cagcgtgagg aggcagagga ccagaatcag cttaatgcag
     7621 aaaatccaga gatacttggg atggtgctgc atgccctgac cagtctgaga cacaaagatc
     7681 tggttgacgt agtggaggtt gatgccgccg gtaaacagga ggaccaccaa caggactccg
     7741 gccacccgct tgtaatgctt gtaaaaggca tcggtgatgg ggcggacata ttccttgggt
     7801 agggcggctt cagcgaggcc cggcttgacg gccatcacaa tgaaggcgag tccaccgatc
     7861 cagatcaccg ccgagaggag atggaaccag tgattcacga tggggatgag ctggttcagg
     7921 tcaaggaaga tgctttgtac ggaatccatg atagatgggc tagggcggat agcaaaggta
     7981 ggggagtcta ccgaaggcct gtctcgcgcc ggctcctagt ccttccctct catatcgaaa
     8041 ttgaagacag gcgcatcgtt gccgaaacgc ttcttccgca gctcttccat caattcgatg
     8101 gtcacgaggg cgatgccgtt ttcacgagca tgacgttcga cgcccttttt caccatcgcg
     8161 cggagaaaat agggaatccg gctgagccga agctcagagg cttcgtccca tgcaacttcg
     8221 ctttcttcag gtgtgttgaa ggcgactttg atcttgtcgc cgcctttcgg tgtatagagg
     8281 caccactgat cctcatccag ccagtcgcca tgatccacgt aaggacgagc ccgacaacca
     8341 ccacagatgt cggtgtattc gcagtcaccg catttgcctt tgagttgagg gtagcggaag
     8401 gagttaaaca catccgaccg ttcccacaaa tcggcaaaac tcgtctcccg aacattgccg
     8461 gccgacagcg gcatataagg acagggggtc agttctccgt ttggcgtgac acgggcatag
     8521 ttggtaccgg caaggcaacc tccgcccatg tacccggtgg ccttggttag cggggaattg
     8581 ggatctttct cgtaagccag gcgcttgaaa tgcggagcgc aacgtgcccg aaccagcatg
     8641 tctttatatt ggtcctggca gtccaccaga tatcccagca cttcttcata ctgctgtggc
     8701 gtgatatcgg tcagctcttc gccgcgtccg gtgcagacca tgaaaaagac gttcaggact
     8761 cgtgcgccca gggtatgggc ccattcgact accgcgggca gctcctgata attcatgggc
     8821 tgggcactga aatgcacctg gaactgaagg ccattacgct tgcaggcttc aatgccggcc
     8881 aatgcactgg caaaggcgcc gggcactccg cgaaagtggt cgtgtttctc aggattgagc
     8941 gaatcaatgc tgatgcccac gcccatcacg ccgatttcaa ccagcgtctt ggccatgcga
     9001 tcattgatca gcatcccgtt ggtcccgaac acaacgataa agcccagccc tactgcgtgg
     9061 cgggcaatgt cgagaatatc cggacggaca agcggttcac cgccggtgat gaccagcagg
     9121 catcccttat ttacttcggc aatctgatcg atgaggcgga agcattcttc cgtgctgagt
     9181 tcatccgatc cgccgccggc tttcgtcgtg gcgtccagat agcaatggtc gcacttgaga
     9241 ttgcatcgct tggtgaggtt cagggcgacg agatacggct tgaagtcatc gaccgtccgg
     9301 ccatcattcg gcgctgtgcc cggtgaagtc ggagagatga acttcgagat ctgttgctga
     9361 agtgactcca gcttgtctcc gagaaaggga atattgagaa ccggcaacga ctttcccatc
     9421 accggttcgc cccggtcttg gacgagcccg tcaggttggc aatcatgtcc ttcagattgc
     9481 cggttttcat ggcatcggcc atgtagccct tggcttgctc aattccctcg gttgccactt
     9541 ccagggtgat gaccgtcgtg ccgatcttgt cagcatgttt ctcgatcaac gccttggtgc
     9601 attcccgcat gaatccttca ggggcccgat ctaaacggcc ttgcgcatcc ggggtccagg
     9661 tgtaaggcga agatggtgct tgagaagcct gaggttgttc agttccgttg ccgttcgccg
     9721 tagcggattc agtgccattg gcggtcggta cttccgccga aggttggcct gcggttccgg
     9781 tgcccttgtt cgcaaagatc ggggtgtagt cctcggacgc cgcttcttga atcatgggat
     9841 tagcgtattc gagggtgatc gtcgtcatgc cgagcttgcg ggcgcttttc tcgatcttgg
     9901 ctttagcccg gcggcgttgg aatcccgccg gtactgcgcg gatcgcttcc ttcgcgtcct
     9961 tcgtccactg catcggaatg tcgtcatagg cgacgtcggt ctcgaggccg ccctccgctt
    10021 tagcggccgc ttcgaagatc gacttgtcga cctgcttgaa tcgatcccct tcggcgctgc
    10081 acacggggca tttcacgggg gtatcgcctt tgccgatata tccgcagccg tcacagacaa
    10141 agtaattctg gctcatgcga tccagatacg cctgagttcc agggctatgg gccggctttt
    10201 cttccacgac ctgcgtcatc atgccgctca gcgtcgagcc catcagatcc tgcgcaacgg
    10261 aatcgtcggc ttgcatcttg tcgcgatcaa tgccggccgc atccaggttc ccgcccaacg
    10321 cacgcatggc gtccatcgcg cccttcggaa ggatatgtcc gacggcggaa tccacgaccg
    10381 tattgctgat gatcgtgtgt cccttctcaa tggcgtagcg gtgaatggcg gttttggcta
    10441 ctccgcgagc aaacaccggg atgcgttcca tccgacgcaa ggcctcttcc gtccaggcaa
    10501 tggtgtattc cgcctgtgta tcgatcggcg ggacgtattt ccggttggaa accaggatat
    10561 tgcaggaggc agagcggagc aggttttccg tgttgctgcc gatgtccatg tcttcatcgc
    10621 tgtggacgcc gattcggccc acgatgagca gccagggatt ctctttccgc acgtattgaa
    10681 tgattttttc gaaggccttg ccgtctagca gggtcgtttt tacatcggtc tgttcggctt
    10741 gggcaatttc gcgggaaata tcgaggtgcg attggtagat ctttgccaga ccactgtcga
    10801 tgacttcctc gtgcaacttt tcctgctctt tgaagcggaa caccttgccg gcttcttcat
    10861 tgagcacgcc ggagatgctg tgaaaggccg cataatggaa gtagggatcg aaagcggaaa
    10921 tgacttcaac cgggacattg aaggctttgc caagtgccaa accggtcatg agtccgccga
    10981 aggagtaggg gctgccgtcg acagcgacga cgattttccc cgtgccaaca ggcgtcggct
    11041 gcttgatgat cagcatgtcg gagttgcgca cgcgccgcag gactcgctcg gtattactgc
    11101 cgatgacgct gtctttgacg gctccgactc ccaacgcgcc catgatcacg agatcgtagg
    11161 cgttcgtatt gatatcctca gccagcactt tccagttgcg tccttcgagt gagcgacgtt
    11221 ccaccggcag gttggcctca ttacatttct tgtcgacgta atcgaggtac gaatccgtga
    11281 tgatctgcag tccgcgggtg atcagcgagt catggatttg gcgctgccgg tcgagttctt
    11341 tctcgtcgtg atattcttcc ggaaggccag cctccatctg cttaaatcgc ttatcgtgca
    11401 tcttcgccgc gtagacatgg ctacccacga tctttgagcc ataggttttc gccagctcaa
    11461 ctccgacatc gacggctgta ttggaatggt cggaattatc gaccgggata tagatcgtct
    11521 tgtacatcga aacgcctcct aggcaaacac tctatagggg gatgagatca gagaaccgcc
    11581 aagaattcaa acggttgaat ggaactttac cccaaaagaa gcggcatgat agcaccgtgt
    11641 tctcggggat tgcaagcaaa ccgacggtgg tcaaagggtc tgtggatagt cgctaactcg
    11701 ttggactcac agccgtcttt tccgttgcgg cggctgttgg ttgcgcgggg cggcgtgatc
    11761 gaaggatctc ttccaacgtc aagagcgctt cacgtcccga gttattctga atcccccgaa
    11821 tgagattcgc atcggcataa tgagcctggt ccggcttcgc tccggcgggg ggtgtttgag
    11881 aaagggatcc ggtccatgtg cgaggatcgc ggctgccctc tttctgttcc caggcggtca
    11941 ggcaagcttt ggcgatgatt ttgttgagcg gggccgggtt gtaaagcagt ttacgaatgc
    12001 tggtcggcgt caacatgagg gggttatatc cggctgaaag gtagccttct tccagcagcc
    12061 ggtgttccag atccgtattg ggctggatac cgaggaagaa catcagcggg aacacacgct
    12121 cttcacctaa aattgacgca acttttttgt aggactccac gctttgaagc agactttctt
    12181 ccgtctcctt cggagagttc agcgagtaat tcaggatcac tttccctttg aaaccggctt
    12241 cggccagata ccggcatccg tcatagagac gttcgagttt gaagcccata tggagattgt
    12301 tgagcacctc ctgcgatccg gaggtaatgg cgacttccaa gtctccgacg cccgatcgca
    12361 ccatcagctt agccagctcc ggagtgatca gcgaggtgcg aatatagccc gaccactcaa
    12421 tctccagctt ttcgctcagg attcgttcaa gaatctctgt gcattgagga taggcttcct
    12481 tgcccgtgat gaactgggca tcggtgaacc agaatcgacg ggcgccccac tgatgatagt
    12541 gctgagagat atctttgacg accatttctg gcggccgata ccgcacccgt ttcccctcga
    12601 tgtagggata gaggcagaaa gcacaatcat aggggcagcc gcgtttcgtc tggacgccga
    12661 tcgattcgtc catgtacgag gcgtgctggg gaaaaataga cgtgaggtag ggcagatcga
    12721 cggtcaatgc gtccagcagg gccggggagc cctgatttcc cttacgcgtc tgtttccctt
    12781 cccgaatgat atagcgttcg ttttcaagcg attcgccgtt gaccaccttg aggattgcgt
    12841 cttccccctc gccgaggatt ccaatagtcc cttccgggag cttctcgatc aattgatcgg
    12901 caaacgccgt gaacgctccg ccgccgatca tgatctgggt cttgggaaat tccttccgga
    12961 tcaaccaggg atacgacagg ttcgcgcgaa tgtggctgta ataccggtag agctgcttca
    13021 agccggcgaa tgacgcggcc acccgcttga tcggattgct ggcgaagtaa aaattgaagg
    13081 cgtgctccag cgaggcgtct ccttcgtggg gggagaaaat ctgaatgtcc cgccacgaaa
    13141 aacagaccag atccggcttg aactccagcg cggcatcgcg gatggcttgc gtccgttggg
    13201 attcggaaaa cagcgacaga tcgagaatgc gctgtcgcac ctcgggtttc cggcgatgga
    13261 taaagtctgc cagataggtc acgccgatcg ggtagacctt tttgcagggg agaaaaatgt
    13321 agaggatgga attcatcagc tgtcagccgt cagctcgtag ccatcagcct gtgctgagag
    13381 ccgaaagctg atcgcgtggt tattatttgg ttgctacgtg gatggcgacg atgcctccgg
    13441 tgaggttccg gtattccact tcgcggaaac cagcctccct cagcagtgtg gccaatcgct
    13501 cttgatcggg gaaagtcctg atcgaggccg gcaggtattc atagacgccg gtccgatcac
    13561 gcgccaccct ggtgccgatc cacgggagca ggcgaaacga ataccaatca tagagcgcac
    13621 gtaaccagcc ataggccggc cgggaaaact ccagacacac gaaacggccg ccaggtcgaa
    13681 gaacccgccg gatttccgtg aacgcctggg tcaggttacc cacattgcgc atgcagaagc
    13741 cggtcgtcac tgcatggaac gtattatcgg gaaagcccag ctgttccgca ttggcctgca
    13801 ggcaggtaat gcgatcgagg agccctctcc ccgacacttt gcggagccct tcggctaaca
    13861 tggcatgatt taagtcggag gccaccacat ggccctgttc gcccattttg ggttcgatca
    13921 gcagggcgag atccgccgtt ccggcgccga tgtcgagtgc acggccgttc gttacggtcg
    13981 gcacatacga agccgtgagg cgcttccaat ggtgatggag accgaagctg agcagggtgt
    14041 tgttgaggtc gtagacgccc gcgatcgcgg tgaacatgcg ctggacggcc tgttcacgag
    14101 cctggccgga ccaggttgag acggctttcg ccgccggttc ggttatcgga gattgttctg
    14161 cccgcaccat gggagggtgg tagcacccgc ttttgcccgt tgtcaaggtc gcgggtctgt
    14221 gcggacggag atcgaaatcg attggctcgc cggggtgccg gcgttaggcg gcggcgcggg
    14281 tctcgctctt cggggccgac cctttccact ggccgacttc caggctttcc agccaatcga
    14341 gcagtttgcc gacgttctct ccttcgaaaa tcgatccatg ctgcgggcag atcatcttgg
    14401 gcttgatggc acgcacccgt tggacccatc cacggagcgc cgtggtagag ggcatccagc
    14461 ggagatggaa cccccgcatg tactggatgt gctgctcgaa atccgtcaca agcaggctgg
    14521 catggtggtc cgggacaagg gcgccgccga tgtcgcccga gaacaagatg cctgcactgg
    14581 ggtcgtagag agagaagttg ccggaggagt ggcaataatg ggctggaacg gcttccattt
    14641 caggaccttc ctcgccgatc ttgatgcgca tgccgtcgtc gggaatcggg tttaatgtga
    14701 tgacggcgcc ggtgctgaaa tggctgacaa agccggtcca gagccaggag caataggtct
    14761 tgatcgccgg attcaggtcg agccacatgg cgagcgagga gcagatgtcc gggtcctggt
    14821 ggctggcaaa gatcaccttg atatcctgca tgcggacata cttcgctaat tcggccagaa
    14881 cctgggggaa aatctgaatg cctcctgggt ccagcagcat ggcatggccg cgatggatga
    14941 tcacatattc gttggtgtcg atgacgtggt catccttttc ggggtcacgg ccgatgacga
    15001 cccactgatg atcgtcttta ttgaacaaca ccttactttg cataggtatg ccctttcagc
    15061 agcagtccaa ggagctcttg cccatgttcg atagcatcct tcatcgcgcg ggtcttgtta
    15121 tcgaggtcgt cgacgacccg gctgatttcg gctgccagat ttttgaagtt ggcctccgcc
    15181 tccgccagcc ccgccgcctc agaagagatg cagaacgcca tatagcgcgc ggtgagtcca
    15241 atcattttca gcccgttcag cgcctgatgc gtttccccca gacaccgcag cgcctccttc
    15301 agccgttcct gtaactcagt gaggttcgcc tggcaatgat gcaccagata caagcggttc
    15361 tcccgctcca ggccccccac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 tcgcctggca atgatgcacc agatacaagc ggttctcccg ctccaggccc cccaccgctg
    15541 tgaaggcggg atgttgcgtc aattgggtta cgtcggcggc agtggtaaag cgaaggtgcg
    15601 agagggtctc caagctcttc gacttggaag agagctcccc ctgtgtcatc gccgaaagca
    15661 gactggcaat caattgatgg tatcgccaga caatgtttga actgctggcg gtcaccttgg
    15721 ccagcccggc acagagatgc tccagggctt ccacttccgg ccggatttcc ttaggggtgt
    15781 tattcaagat ctcaaccagc gccagcatgg gccggccttt gcccccaggg attttggccg
    15841 cttgcaccac gccattattc gcgagaattt gcatggagag cgacagttgt tggagagagc
    15901 ggcccacgtt gaggatatcg acggtgatcc cgaacagttg attgatgttg cgtttgaaga
    15961 ggctcagcac gtcatgcatg gcggccatcg aaatcggggt ggttgtggat cggggcgaag
    16021 gttcccgcag ttccgtatcg gcacattcgt ggaaaactaa aatgccatcg ttcgatgctg
    16081 gaacaccgcg tcgacatttt gacggtcgtc tgagcatgag cgtcaagttt aaggtgtcgg
    16141 atgccaagcc tcgttgcccg gcgtgatata atcccgcgct tatgagcaat tccatcatca
    16201 tcaaaggggc gcgtgaacat aacctgaaga atctcgatgt cgagattcca cgggacaagc
    16261 tggtcgtgat gaccggcctg agcgggtcgg ggaaatcttc cctggccttc gacacaatct
    16321 atgcggaagg gcagcggcgg tatgtcgagt cgttgtccgc ctatgcccgg cagtttctcg
    16381 aacagatggg gaagcccgat gtggactcca tcgaagggtt gtcgccggca atctcgatcg
    16441 aacagaagag caccagccac aatccccggt ccaccgtcgg aaccgtcacc gaaatctacg
    16501 actatctgcg tctgctgtat gcccgtgtcg gtcacccgtt ctgtttccaa tgcggacagg
    16561 aaattaccgc gcagacggta cagcagatgg tggatgccat ctgcgaactg ccggaggggt
    16621 caaagtttca gattctctcc cccatagtcc ggggtcggaa aggggaatac cgcaaagaac
    16681 tgctggagat gcgcaaagcc ggatatgtgc gcgccaggat caacggcgag atggtcgatc
    16741 tgggcgagga catcaccctc gacaagcaga agaagcacac catcgaaatc gtggtcgatc
    16801 ggttggtgat gaagccgggc gatgccttga tgcggcggat cgcggattcc gtcgaaacgt
    16861 cggtgaagct ggccggagga ttggtcgggg ttctgtcgga gaccggcaag ctgcacctct
    16921 atagcgacaa gctcgcctgt atcaaatgcg gagtcagtta tcctgagatc acgccgcggg
    16981 tattttcgtt caacagcccc cacggcgcct gcccggcctg cgacgggatc gggtatgcca
    17041 tgactcccgg tgccacggaa gaagacgact tcaccctgct tgaacggtgc gagacctgtc
    17101 agggcgcccg tctcaagccg gaaagtctgg cggtgaaaat cgccaagaag tctattgccg
    17161 agatcaccgg tctctccgtg cgtgccgcgg cggatttttt cgccacgctc aaattcaccg
    17221 aacgtgaatt ggtcatcgcc catcgcatcc tgaaggaaat ccgcgagcgg cttgggtttc
    17281 tggtcaatgt cggattggac tacctgacgt tggatcgtcc ggcggcgacg ctctcgggcg
    17341 gggaggggca gcgtattcga ttggccacgc agatcgggtc cggtctggtc ggtgtgttgt
    17401 acattctgga cgaaccgtcg atcggactgc atcaacggga caaccggcga ttgcttcaga
    17461 ccttactccg cctgcgcgac ctgggtaaca cggtgatcgt cgtcgagcac gacgcggaga
    17521 cgatggaggc tgcggactat attctcgacc tgggcccagg cgccggcacc cacggcggcc
    17581 gaatcatcgc ccaggggact ccgaaacagg tcaaggcgaa ccccgattcg ttaaccggcc
    17641 tctacctgcg cggcgcgcaa atggtgtcgc ttccccagcg tcgccgcacc ccaaaagggt
    17701 tcgtcaccgt cgtcggcgcg aagaaacata atttaaaagg cgtgaacgtc aatgtgccgc
    17761 tcggcctcct gacctgcgtg acgggtgtgt cggggtcggg gaaaagcacc ctcgtgctcg
    17821 aagtgttatt ccactcgctg tcgcagctgc tttatcacaa gaagcccaaa atcgacggct
    17881 gcaaagagat caagggcgtg gacgcgctgg ataaaatcat cgacatcgat caatccccga
    17941 tcgggcgcac gccccggtcg aaccctgcca cgtacacagg actcttcacg ttcatccgcg
    18001 atctgttttc caatttgcct gagtctcgcg tgcgtggcta caagcccggc cgctatagct
    18061 tcaatgtcaa aggcggacga tgtgaagcct gccagggcga tgggctgatc aaaatcgaaa
    18121 tgcatttcct gcctgacgtc tacgtcacct gcgaggtctg caagggacag cgctacaacc
    18181 gcgagacctt ggacattcaa tacaaaggcc gcagcatcgc cgacgtcctc aacatgaccg
    18241 tggatgacgc cttggagttt tttgaaaaca tcccctttat caagtcgaaa cttcagacgc
    18301 ttcacgacgt gggcttgcac tacgtcaagc tggggcagtc ggccaccacc ttatcgggcg
    18361 gcgaagctca gcgggtgaag ctctcgcgcg aactttccaa acgccccacc ggccgcacca
    18421 tgtacattct cgatgaaccc accaccggcc ttcacttcgc cgacattcaa cgattgctgg
    18481 acgtgctcga tcgtctggtt gagactggga ataccgttct ggtgatcgaa cacaatctcg
    18541 atatgatccg caatgccgat tggatcatcg atctcgggcc agaaggcggc gaccgcggtg
    18601 gcgaaatcgt cgcggaaggc ccgccgaaag aaatcgctaa ggtgaagcgg tcgtatacgg
    18661 gacaggtgtt aagagaggcg ggggtgtaag ggatggagtc ttcggagtcg gcatgatgcg
    18721 aaggatcgaa gttgcttccg atccgctccg gtgccgtgtg cgactgtgag gcgcgaccga
    18781 aagtcgcatg ccgtcgcaca acggcaggtt tatccgctac atctttccac acccgccatg
    18841 ccgacttcca aaggctccat cccttacacc ccgctaagag agaaggggag cgggtgatta
    18901 ggaaaaaaga ggtaggagaa gagacggaca gtccatgaga gtccacagtc atgcgttggt
    18961 tccagccgcg tgccactcta atgcgcgaca aaagggttgc aagacgccgc actgtgacag
    19021 gttatccggt acatctctcc acgcccgcca taccgcactc tgaaggctcc acccttccac
    19081 ccccgcaaga aaggaagagg ttggccgccc gcagtggagg gtcggcgcag tccgcccgcg
    19141 caggtgatcg agcaaaaggg caccatcgga tcaatttccc ctgaggcgag tgaaaatgcc
    19201 cttactcatc cgaatcctga ttgtgggatt tctcggtctt ctcaacggct gttatgttgg
    19261 tgagggaacg gggtatcttc ggttcgaagg aaaagtgagc aacgaagagg gccaaccttt
    19321 agatgggcgg gaagttcgga tacagttgtc tcccgtagaa tccattcatg tcagaaatgt
    19381 gggcaaggta gcacacacac aaaacgatgg gaaatttgta atagaaaccc atgggattgt
    19441 cggtgtcgtt tatgtcgctc ccatagggtg gattgtggtt ccccttgggg tagttattgc
    19501 ggctctgacc ccttggacga atagcgaggt gcaggatttt ttgcgcccgt cctatacctt
    19561 tggacctgac tgtctcgacc atccaaagat aattgcactg aagatcgatt ctgccgacta
    19621 tggaatgacg tttatcgaga aagatattgt tcgaaagcta tatcatggag tggaacgaaa
    19681 gaacggagaa tcctgcgcga gggtttatga tttcggcgaa ctagaaattt cgatgggctc
    19741 gctgtcacgc taagcgatgc agagcgagct tctgctgtag gatctccctg aatcatagat
    19801 gaacaaggag gcccatgcac acgattctct tactgaccat ttccaacatc ttcatgacct
    19861 tcgcctggta tgggcatttg aagtacaagg agtcgccgct gtggatcgcg attcttgcga
    19921 gttggggcat cgcgttcgtg gagtactgtt tccaggtgcc ggctaatcgg atcggtcatt
    19981 atgagttcac tgcggcgcag ctcaagacga tacaggaggt cataacgctc atggtgtttt
    20041 gcgtcttctc agccgtctac ctcaaagagg aactcaaatg gaactatctg gtgggattcg
    20101 gcatgatgat cggtgcggta tttgtcgtct tcaaagaatg gtgagagtgc ttcgttaggc
    20161 atttcccctg tctgctcctt ccttcttttt cccagtcatt cctcgtttcg cgctgtcgaa
    20221 ccgttcattc accggctcgg tgaagccgct agggcctttc tgttctttct cctagggtaa
    20281 taactaggct ctccttctcc cttggccagg tacaacaaag tgtgcactgt gttgaatcct
    20341 tcgcctttgt gggcgagaca agccaaggag gtctgtatga agtcatcatc ctcaattcac
    20401 aacctggcct gtgccctctg tgtggcggcc gccggcttga tggtcggggc ctgtaacacc
    20461 accaaagcca cggtcgatac caccgtcaac tttttctcca gcacgagccc gaatgatctg
    20521 ttcacctccg atggcatggt cgtgaaggaa cagaagatca atttgttcgc gggcgtcgcc
    20581 tatgaaaacc tgcggcagga agctgcgacc ggcagtgggc agtatgtgac cgcactggcc
    20641 gcgctctacg aaattcccgt cagcaagcag caggagttcg ggcaggtcct gcagcgcaac
    20701 catgccgatt tatttgcggc ggatcttcag gacgatcaca gtgcgcacct caagatggtg
    20761 tcggcattga atcgcgaact cacggcggcg gcattattgc cgtgaagcag cattcgcaag
    20821 gaggcatcca tgggaccgat gggcaaaggg ttcatcctct ggttattagg cgtgccggcc
    20881 agcattctcg tgctgctgtg gtttctcgga attttgaagt gatgtgccaa gcgttgagcc
    20941 agtacaagac tcacattaag gaggggtgat aatgcgacga caacatatat ttgccgtagc
    21001 ggcggtgctg gccttaacgg gtctgccagc ttttgccgct gatctcgaag tccaggtcgt
    21061 ggacaagaat tgcttcatcg aaattttcga agacgataac ttcgatgtgg acgatccgca
    21121 cgtcgtgctc caaggtccca aagagtatgc cacgttgaag aatctggccg ggaaggattg
    21181 gagtaatg
//
LOCUS       DAOQDS010000063        21167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_946_length_21167_cov_19.301487, whole genome shotgun sequence.
ACCESSION   DAOQDS010000063 DAOQDS010000000
VERSION     DAOQDS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 21167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21167
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_946_length_21167_cov_19.301487"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..620)
                     /locus_tag="PK589_14295"
     CDS             complement(21..620)
                     /locus_tag="PK589_14295"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ20828.1"
                     /translation="MKEAIGVDHITLCVKNLSATKYLFTHILGFDVIWSAQDVGSEKS
                     SMDTVVVQRGAAKIALMQGRNKERKSQICEFVETYGEGVQHIALEVDDIEAVCREWEH
                     HGVRFSGDIKDGRDGFGPLRQRFTYPLFPGCGLFLELTQRQHGTEESKTFVRATVESL
                     YRDIERDQIKGVARTIIDYETLPLPFEEEVEAASLRHAS"
     gene            835..1593
                     /locus_tag="PK589_14300"
     CDS             835..1593
                     /locus_tag="PK589_14300"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNJ20829.1"
                     /translation="MSFYEQVRSDVLRHGAINNSYLDRFLAGDVSDKEFREFAVEFYN
                     FSRFFPRILAAQLVNTEDEAVADELTKVLYSELGDGSVKHRHELLYRNFLRSLGIDIH
                     VAMTTPMRPSTKAYIEGMERLYGSGNHAMALGASFGLENMAITMWDHLIPGLTRLRAE
                     RYADMDMTYFVFHRELESEHERAMQHAIVAVDGDSHAPHARMSDKEKDDFRLGMNAVL
                     DYLEGFWMGLERKPAGVAQSRSRPVHGLESELRG"
     assembly_gap    1793..1892
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1893..3180
                     /locus_tag="PK589_14305"
     CDS             <1893..3180
                     /locus_tag="PK589_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ20830.1"
                     /translation="HRIILTSLRDFLPPLKRWLYPIKARWAKRWISVEKYPPVYDFCA
                     LLDRGARERDVDTEPLPTLEPGALAQIQYTGGTTGIPKGVMLTHRNVVVNTMQGRFWC
                     ANFREGKEVFLGAVPLFHCYGLNTCQNLAIATGSQIILLPRFHADEAVKAIQQHRVTI
                     MSGVPMMFSMMTDSPNVRRYDLHSIRVCLCGASPLPAEVQDAFERLSGVKISEGYGLT
                     EAGPTTHCNPIERDHPPGSMGLPFPDTEARIVDLETGLTDLPTGAAGELIVRGPQVMR
                     GYWQKEADTQAVLRDGWLHTGDIVRRDEQGFFFFLDRKKDVIKPWGETVYPREVEEIL
                     FQHPAVHEVVVVGMPDRHYGEAVKAFVVPVEESSVTERELIDHCRKSLARFKVPVAVE
                     FRSELPRTIIGKVLRRALRDEAEVPVGTAQAPRKAV"
     gene            3200..4390
                     /locus_tag="PK589_14310"
     CDS             3200..4390
                     /locus_tag="PK589_14310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNJ20831.1"
                     /translation="MREVVIAAAARTPIGNFGGALKDLTPHKMGELAVREVMARAELD
                     PGLIDEVIIGSVGHTSDAYNVARVIALMAGLPVRTPAYSVQRNCSSGLQPFVNAYQNI
                     QSEDADVQVVGGVESMSRAPFVSRDMRWGKRLRHAEFIDSIWEGLTDAFCGQLMGLTA
                     ENLAEEFNISHEEQDHFAVESHRRAFKAIREGRLKDEVFPVMVQKTVAGREVPPVAVV
                     QDEGPNVGLTEQQLALYPPLFKEGGTVTAGNSCPLNDGAAAAIVMSGARARDLNHRPL
                     GRIKSYAFIGVEPTRMGIGPAEALPLALKRAGITLTDLNLIEVNEAFAAQYLAVERVL
                     ELKRDIVNVNGGAIALGHPVGMTGTRLVMTLLYEMKRRGAALGAVAMCVGGGQGAAMV
                     LEQI"
     gene            4465..5712
                     /locus_tag="PK589_14315"
     CDS             4465..5712
                     /locus_tag="PK589_14315"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HNJ20832.1"
                     /translation="MYIYKVGVVGAGTMGAQIAEVVSFAGLPVVLADREEALARRGVE
                     SVRAIYQARVAKGKMTPEQLEEKMLLVTASPNLERLHDADLVIEAVSEDLLLKQRVFQ
                     ELDRICSRSAILASNTSALSISAIGSATKRPDKVIGLHFFNPAYAMPLVEVIPGLGTD
                     PQTVDDVVGFSESLRKQPVIVKECAGFLVNRLLSPYLNEAVWCLQDGDVSIKEIDQDM
                     VSFGMPVGPFTLLDTVGLDIALDVARILHRSYGPRMAPAPLLEAFVKAGRMGLKTGKG
                     LYDYGAAEEGGADQHLERLRAQARQLTGRSSMKWSPLRPLVAMVNEAVIALQEGVASA
                     RDIDFAMAAGTGFPSDKIGPLHLADQLGIDRVLQELEELQDRVGPRFWPAPMLRRLVD
                     AGFTGQGAGRGFFSYEPTIARMR"
     gene            5720..6508
                     /locus_tag="PK589_14320"
     CDS             5720..6508
                     /locus_tag="PK589_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNJ20833.1"
                     /translation="MTTTAGSMLACHRDGAVATVTIHHPPANTLTPELLGELSQAFEM
                     FATDEAIKVVVLTGTGRFFIAGADIRLLAAIASSQEGASIARQGQAILNRIESLEKPV
                     IAAINGVCLGGGMELALCCHIRLVAEGSRLGQPEINLGIMPGFGGTQRLPRIVGQSKA
                     LELILTGDPISAQEAKALGLVSAVFPPEDLLHQAHSLARKMASKSLPALRASLRAIRS
                     GADMSLPEGLALEAVLFGGLCETEDKREGLAAFLEKRQPRFIDR"
     gene            6545..7765
                     /locus_tag="PK589_14325"
     CDS             6545..7765
                     /locus_tag="PK589_14325"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNJ20834.1"
                     /translation="MASAFGKRALALGGGGFTGYLFEIGALTALDDLFEDGVSMNDLD
                     LYVGVSAGAAAVSLIANGVKPRDILETNLSGTRPYYFDHRNVFSPAIGEGLKTFWRVT
                     RQLVPLVKYYVRHYGEMTLIDLLDKAQDALPSGIYTLEPFALYLAGLYKTKGLSNSFA
                     GLSKELYIPAIDLETGENVLFGEEGWQEVPISRAVTASSAVPIYFCPVRIQGRDYIDA
                     GIGRMAFFEVAVHKKVDFMIMINPMARPLQRRLPVDVPDHGSSVGRLRDRGFLSIGEQ
                     ASRINFDARFSQALERFQHEYPAKEVLVISPVEEDALLFERSFLSYRDRIHLLRAGYG
                     SVMTLVRDQFDHVSAQLASYGMTISRARFEDRAVQRLARLNHVAWTSAPRVVPNAATA
                     ARIGGIGAAWRKAR"
     gene            7788..>9535
                     /locus_tag="PK589_14330"
     CDS             7788..>9535
                     /locus_tag="PK589_14330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ20835.1"
                     /translation="MANKDRMFTGSLAVAEAARDTRLYSGFLSGLFEGNVRRHLYSRA
                     SLPARSGAASEFLDRLRHLLIEKVDPETIDREGLIGEDVFAALREIGAFGIKIPRRYG
                     GLDFTQSEYHEAATLLGSHDAATTVLLSAHNSIGAAEPVKLVGNSEQQQRLLPRLARG
                     EVSGFALTEKGAGCDIWDLKTYAIPVHEQGLVVGYRLTGDKLYTTNAPRGHHAFLASW
                     LVVIAQIVDSPQDIHRAKEERRFGAFVVDTHTRGCSCTRLSFMGVRGIYNGQVHLREV
                     WVPVADRLGAEGEGLRRALESLTVGRLTLPAACLGNLKQCLWLARLRAQQRKQYDRPI
                     GEHTDIGAKIVLMASRVLALEAIVKITGIWADTKQDVRLESAAAKILATEWLLESLLD
                     LFRIYGGRAFETPDSLRLHGDLPAPIERMIRDALINVIWEGSNGILTLWIGREGLSQY
                     FTHGQAFLDCHIADMVRATPFFVNTAARSFGGLTVYEQDSEAGSFDPLWERFVARKSR
                     ELARTTLWVAARDRQGLARKQRLMTRLVSAAMHLFAVETALWYKSRQEQRGKPHLDQL
                     VSYFCSRTSAAFNPEP"
     assembly_gap    9536..9635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9691..10674
                     /locus_tag="PK589_14335"
     CDS             9691..10674
                     /locus_tag="PK589_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ20836.1"
                     /translation="MMEIARYFDAVKDRYDLSSDYALAEKLGIAQPEANLMRRGLKIP
                     KPELCIKMAKLLDKNPVELLLIAQKDKAPKQAKEYWTLALTAVDVMLHVPKRPRYLPK
                     KVEAIGRELKQLEAQTLTYEGAAANAEAVRLMETAEQSVDAMMERWNIWKKGEALYPN
                     YLLANQAAARRHVRIRRLLILTQEQMQHDATVADAIQVMDDQRRAGVKVFYAFREALV
                     HSPAFQRLEEDFRKYGAAEDMNTAMFDREILIFSQTYGSVPLGMVGTPTPITMINRLQ
                     ISWKPEMIRELDPAPLFDMTRYVFDYQGSGPFHEQLAYFRRSIRELPIRAV"
     gene            10649..12076
                     /locus_tag="PK589_14340"
     CDS             10649..12076
                     /locus_tag="PK589_14340"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNJ20837.1"
                     /translation="MNFPFEPFRIKVVEPVKRTTREERDRLLREAGYNLFHVPAESVA
                     IDLLTDSGTSAMSDSQWAGMMLGDESYAGSKNYHHFETVVRGLFGYRYVIPTHQGRAA
                     EHVLFSTVLKPGMCVPNNIHFDTTRANVEHQEAEAKDLVIKEAYDPHCELPFKGNMDV
                     ARLEETIKRVGPSRIPFVMITITNNSAGGQPVSMANIRATRQVLNRYGIPLFFDACRF
                     AENCFFIREREPGYADRPIRDIARELFRLGDGCLMSAKKDGLVNIGGFLALNDERWAQ
                     AVTNMLILVEGFPTYGGLAGRDLEAMARGLEEVLDEEYLRYRIGQVQALGALLEEGGV
                     PIVKPTGGHAVYVNAKEFLPHLQQSQFPAQALAVALYRDYGVRTVEIGTVMFGKRDPA
                     TEETIYPALELVRLAIPRRVYTSQHLSFVAEALVDLYQRRHSIGGLVMTYEAPRLRHF
                     TARFEEETLAEMLWPGAGFAAAPLS"
     gene            12499..14307
                     /locus_tag="PK589_14345"
     CDS             12499..14307
                     /locus_tag="PK589_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNJ20838.1"
                     /translation="MACVKKSVGQASGIYGAADARLDASGRSSNRGDVLRRWRGTVAG
                     VAAVLLLGALVVPAHSQTGSQLGPLPPKPPQLPVVPPPAQPPALDLPTPTPPGALDQA
                     AKGPKIMVKDIRLVGNTAFTSQQLSEITAPYTNRELTAEDLESLRLALTYYYVNHGYL
                     TSGAVVPEQDVADGTLTMQIIEGKITQVNVEDTKWFRPSYFQSRVNLAAGPPLQVEAL
                     QERLRVMQANPRIERINAELLPGATIGENTLNVKVKEANPLKAWLEFNNYQSPVVGAE
                     QGFITLAHQNLLGFGDTLSLQYGRSAGVNPMLNFKYEIPVGPRDTTVALQYRRFDFGV
                     EESPFDSLDIKNKAQIFGLSVRHPVIRKADQELAFSLTGEHARNESTLGGNPFELITG
                     APNGRFRVTALRFGQEYAQRSAEQVISLLSRFSVGIGAMGATANADPNLPDARFFSWL
                     GEAQWIRQLPFWRTQLVSRGVVQLSNDHLFPLEQIAVGGRYSVRGYREFTLIRDNAAM
                     ASIEARVPVYTTKAGVDSVFLAPFFDFGHGWQTTAQTPGTPPKTLASLGAGVIWNFWR
                     GSHFELYYGKQLKRYDTDRNNLQDHGIHLQLVVEAF"
     gene            14588..15865
                     /locus_tag="PK589_14350"
     CDS             14588..15865
                     /locus_tag="PK589_14350"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNJ20839.1"
                     /translation="MLCRPAGLPLLVRGYLAFLMLLGSTASIAYGQATNIVATPGGPG
                     GLGTTLNTAGNTTNITGGTRPGGGPNLFHSFNQFSVGAGDVASFVNPGGVSNVISRVI
                     GGSPSNINGTVQALNANLFFLNPSGIIFGPSAHLNVSGSAYFSTAQQLRLSDGGIFTA
                     STSLLAFDSTLSASSPVAFGFLGQGPYGSIVLTSSSTVLQTGAVLGLMGGGIQINGSK
                     ISAQRVMLGSTSSAGEMSTQPFVGNPFSGAAGNGQVQALPGTLIVAGGGGVIPASIDV
                     SPNVTVPTGAQVNTTSNSVTQRVTQIQVVGVNLGGLPPLPTANAANVNPTNPVEPVAF
                     LNRAAMVLPQEAPAPPAPLLTSRCAARKDGTFSSLVQASRDVTPSQPGRSLAAPVVLE
                     EIAGDLEPGAPAAPSAHIHGQTIAQLMESWQGC"
     gene            15884..>16212
                     /locus_tag="PK589_14355"
     CDS             15884..>16212
                     /locus_tag="PK589_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20840.1"
                     /translation="MPTMRNMVTRRGMKRSHGAAGSAGPRTLSSPLTILSLGVMALSS
                     FTWPASGMALELLGGSSTSSITPAQEMRQGTTQFQQGAFVQAATHWMNAARGYEETGQ
                     IKEQSQA"
     assembly_gap    16213..16312
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16313..17758
                     /locus_tag="PK589_14360"
     CDS             <16313..17758
                     /locus_tag="PK589_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ20841.1"
                     /translation="YLGGLREQEHRNPEALEYTRKATFAAQKVNAPESLYRWQWQTAR
                     LLRAEGKEDESLAAYQRAVTLLKPIHYEYSVGYQGRHHSYYESVAPLFVEYEDVLLRR
                     AAAAKTPEQNDQLLVKVKETIEVSRAAELQDYFQDDCVATVASHRGAGALAPGTAVIY
                     PIAFPDRLELLLETSNGLKQVRVPVAGEKLTKEIRSFRRLIQDSQSQNYLSSAQTLHG
                     WLVAPIQQDLQGAGVHTLVVVADGSLRTIPMGALHDGRHFLVDSLAVAVTPSLALTDM
                     NGAQRRKGSVLSVGLTESVEGQSAPRYAETEVQAIRTMYGGKLLMNKQFSTPTLEEEI
                     KDQGLGIVHVASHTVVGTEARDSFVLAHDGKITMDRLSQLVGLQQYRQQPLDLLTLSS
                     CEIAAEDDRAALGLTGLAVKTGARTALASLWTSDDETTTELVSEFYRQLQDPAISKAV
                     ALQRAQQKILSQRGHTHPSYWAAFLLINNWM"
     gene            complement(17836..18474)
                     /locus_tag="PK589_14365"
     CDS             complement(17836..18474)
                     /locus_tag="PK589_14365"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20842.1"
                     /translation="MMAILVLSLLGYFALLPSTAQASRTLVVPISVATYTDTQVGFPT
                     FVMKWDEGAQPDPLALRWGRSQVPVRGTGMTSLQHAFQFAVTRLDASIRPTGTLSLYA
                     TFTGPLSEEGSTAEAALAIGFIALLKGDQLRRDITITGTLEGDGRIGQVDQVAEKTTA
                     AARAGYHVLLVPRGQFYAPRVNRVSLTPESNVLVREVETIEEAYEIMTGKKL"
     gene            18793..19650
                     /locus_tag="PK589_14370"
     CDS             18793..19650
                     /locus_tag="PK589_14370"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ20843.1"
                     /translation="MQGAIVITGASTGIGAACARYLDGLGFTVWAGVRRKEDGEALAR
                     STSARLRVLLLDVTDSDSITEASRTLTDALPEAGLAGLVNNAGISVAGPLELLPIAEV
                     RTQFEVNVIGALAVTQALLPLLRRARGRIVNISSIAGLAATPFLGAYCSSKFALEAMS
                     DALRLELAPWGIAVALVEPGAIQSQIWQRATMSATRTLGGVAPESLALYAQPLARMQD
                     VMAGAAARAIPAEAVAQVVARALTVPSPRARYLVGKDARFRAVLKWLLPDSAQDRLLT
                     WFLGLSRHA"
     gene            19698..20120
                     /locus_tag="PK589_14375"
     CDS             19698..20120
                     /locus_tag="PK589_14375"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNJ20844.1"
                     /translation="MAEGGLVRSAGGVVLRQQEVLLIRVSDIKGRPVWSFPKGRLDAG
                     ETPAQAAVREVREETGWSCRIDADLSTTEYWFQREGRRFRKTVVWFKMVPLELTGVPD
                     GEVEEVQWVERTEALRRLTYASDAALLSHALELDPSSR"
     gene            20375..>21167
                     /locus_tag="PK589_14380"
     CDS             20375..>21167
                     /locus_tag="PK589_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20845.1"
                     /translation="MYSLARFSLLEMTECSAVLRQLGEQSRSLQEAASKAVNYLFDTL
                     GDPTTGQRDCVLVRCFKTISYSRLDTEHQQLVREKLGGIAPFPDLRCFTLVASTGAEP
                     DWNRVDRSHRYRVIPMVSADFVKQFPMFSQLLHQFGVSTEFDVRPDGEWLVDLEEKTY
                     NVFYVPDAVGSPYVPVQDGFVGKYGVKSVLCFGGMLPSRDLFAVILFSKTPVPRETAA
                     LCKSLALSLKTSLLAFDEASCTDSGASQQSGSAPEPPASLRHQSRL"
BASE COUNT         4177 a         5756 c         6472 g         4462 t
ORIGIN      
        1 tcaggtgcgg ctcgatgtgt ttacgatgcg tgtcgcaacg atgcagcttc gacttcttct
       61 tcaaacggaa gcggaagcgt ttcgtaatcg atgatggtcc gtgccactcc tttgatctga
      121 tcgcgttcga tgtcccggta caacgactcg accgtcgcgc ggacgaacgt tttggattct
      181 tcggtgccat gctgccgctg tgtcagttcg agaaacaatc cgcagccggg gaataacggg
      241 taggtgaagc gttgccggag agggccgaac ccgtcgcgtc catccttgat gtctccgctg
      301 aaccggacgc catgatgttc ccattcgcgg cagaccgcct cgatatcatc gacctcgagg
      361 gcgatatgct gcacgccttc gccataggtc tcgacgaatt cacagatctg agatttgcgc
      421 tccttatttc tgccctgcat caaggcaatt ttcgccgcgc cccgctggac gacgaccgta
      481 tccatgctgg atttttcgct gcctacatcc tgggcggacc agatgacgtc aaagccaaga
      541 atatgagtga agagatattt cgtggccgag agattcttca cgcacaaggt gatgtgatca
      601 accccgatgg cttctttcat ggcggttcct cccaggtgta gtcacgggga atctcagccg
      661 atgccgcgtg tcagaaaatc ttattccgtg aagcatgagc acactctaca acataagagt
      721 tgcatcattc aatatagatt ttgtattatt tgatcatgat gcatgatagt tcggaaccaa
      781 tcgcgcgcga gcaggcaatg aaacggcgta ccgggacaac ggcggaggtg cacgatgtcg
      841 ttttacgaac aagtgcgctc ggacgtgctg cgacatggcg cgattaataa ttcctacctc
      901 gatcgttttc tcgccggcga tgtgagcgat aaagaatttc gtgagttcgc cgtcgaattt
      961 tataacttct cgcgcttctt cccccgtatc ctcgctgccc aattagtcaa caccgaagat
     1021 gaagccgtcg ccgatgagtt gacgaaggtc ctctattccg agctggggga cggctccgtc
     1081 aaacatcggc acgaattgct gtatcgaaac ttcctgcgat cgctgggcat cgacattcac
     1141 gtcgcgatga ccacgccgat gcggccgtcc accaaggcct atatcgaagg aatggagcgt
     1201 ctttacggga gtggaaacca tgcgatggca ttgggcgcgt catttggtct ggagaatatg
     1261 gcgattacca tgtgggacca tctcattccc gggctgacgc gtttgcgcgc cgagcgctat
     1321 gccgacatgg acatgacgta tttcgtcttt catcgggaac tcgagtctga acatgaacga
     1381 gcgatgcaac atgccattgt cgccgtcgat ggtgacagtc atgctcctca cgctcgcatg
     1441 tctgacaagg agaaagacga cttccgtctt gggatgaatg ccgtactgga ctatctcgaa
     1501 ggattttgga tgggactcga acggaagccg gccggtgtgg cccagtctcg gtcacgaccg
     1561 gtgcacgggc tggagagtga gctacgcggg taacctccct gatcggtcag ggatgggagg
     1621 accagatgcc gcggccttgg atcagccgat atgatcatgg tgtctccgct acctgtgact
     1681 atccggagtg gacagttccg gatttgcttc ggcgctccgc ctcccggttc ccagaatcgc
     1741 ccgccctctt cttctacggc acacgtattt ccttccagga gttgaacgaa ctnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccaccgga tcattctcac cagtctgcgg
     1921 gattttctcc cgcccctgaa acgatggctt tatccgatca aggcgcgttg ggcgaaacga
     1981 tggatctcgg tcgagaagta tccaccggtg tatgacttct gtgccttgct cgaccgggga
     2041 gccagagagc gtgatgttga taccgagcct cttcccacct tggaaccggg cgccctggca
     2101 cagattcagt ataccggcgg aacaaccggc ataccgaaag gcgtgatgct cacgcatcgc
     2161 aatgtggtgg tcaataccat gcaaggccgt ttctggtgtg cgaacttccg cgagggaaaa
     2221 gaagtgtttc tcggcgcggt gccgttgttc cattgctacg ggttgaacac gtgccagaat
     2281 ctggcgattg caaccggctc gcagattatt ctgctcccgc ggtttcatgc cgacgaggcg
     2341 gtaaaggcga ttcaacaaca tcgcgtgacc atcatgtccg gcgtcccgat gatgttttcc
     2401 atgatgaccg attctccgaa cgtccgccgg tacgacctgc attcgatacg ggtgtgcctc
     2461 tgcggagcga gtccgcttcc ggcggaagtg caggatgcct ttgaacgttt gagcggagtg
     2521 aaaatttcgg aaggctacgg gttgacggag gcgggcccca cgacgcattg caacccgatc
     2581 gaacgggacc atcctcccgg ctcgatgggg ctgccgtttc cggacacgga ggcgaggatc
     2641 gtagatctgg aaaccggcct cacagacctt ccgaccggag cggccggtga actgattgtc
     2701 cgcggccccc aagtaatgcg gggatattgg cagaaagagg ccgacacgca ggcggtattg
     2761 cgagacggct ggcttcatac gggagacatt gtcaggcggg acgaacaggg gtttttcttt
     2821 ttcctcgatc gcaagaagga cgtcatcaag ccatggggcg agaccgtgta tccccgggag
     2881 gtagaagaaa ttctctttca acacccggcg gtgcacgaag tggtggtggt gggcatgcct
     2941 gatcgtcatt acggagaagc cgtgaaggcc tttgtcgtgc ccgtagaaga atcgtccgtg
     3001 acggaacggg agctcatcga ccactgccgc aagtctctgg cgcgattcaa agtgccggtg
     3061 gccgttgagt tccgttccga gttgccgcga accattattg gaaaggtgct gcgaagggct
     3121 ttgcgtgatg aggctgaggt tcctgtcggg acggcacagg cgccgcgtaa ggccgtgtga
     3181 gagtggaagg gaggcttccg tgagagaggt cgtcattgcc gccgccgcgc gaacccccat
     3241 cggcaacttc ggtggagcat tgaaagatct cacgccgcat aaaatgggcg aactagcggt
     3301 gcgtgaagtg atggcacgtg ctgaattgga tcccgggctc atcgacgaag tgatcatcgg
     3361 ctcggtggga cacaccagcg atgcgtacaa tgtcgcgcgt gtgatcgcgc tcatggccgg
     3421 tcttccggtg cggactcctg cctattcggt gcagcggaat tgttcgtcgg gcctccagcc
     3481 gttcgtgaac gcgtatcaaa acattcaaag cgaagatgcg gatgtgcaag tggtcggggg
     3541 agtcgagagc atgagccgtg ccccgttcgt ttcacgcgac atgcggtggg gaaagcggct
     3601 tcgtcacgcg gaatttatcg acagcatctg ggaagggttg acggatgcct tttgcggtca
     3661 gttgatgggc ctgacggcgg agaacttggc ggaggaattc aatatcagcc atgaggagca
     3721 ggatcacttt gccgtggaaa gtcaccgccg ggcgttcaaa gccatcagag aaggacgtct
     3781 caaagatgag gtcttcccgg tcatggttca aaaaacggtg gccggacgcg aggtcccgcc
     3841 ggtcgcagtg gttcaggatg aaggcccgaa tgtcggcttg accgaacagc aactcgcgct
     3901 ctacccgccg ttatttaaag aaggcgggac cgtgacggcg ggaaacagtt gtccgctcaa
     3961 tgatggtgcc gcggccgcga ttgtcatgtc gggcgcccgg gcgcgcgatc tgaaccaccg
     4021 gccgctgggc cgcatcaaga gttacgcctt tatcggggtg gagccgaccc gcatgggcat
     4081 cgggccggca gaagcactcc cactggcgct caagcgcgcc gggatcaccc tgactgatct
     4141 gaacctgatc gaagtgaatg aagcctttgc ggcgcagtac ctcgcagtgg agcgggtatt
     4201 ggaactcaaa cgggacattg tcaatgtgaa cggcggcgcg attgccttgg ggcacccggt
     4261 cggcatgacc ggcacgcgcc tggtgatgac acttctctat gaaatgaagc gtcgcggtgc
     4321 ggcgctgggc gcggtggcca tgtgtgtcgg cggtggacag ggcgccgcca tggtgctgga
     4381 gcagatctga ggaagagtgt tgagtgatga gtgagtgagc tgcagtgacc aagatggacc
     4441 tcaccgggaa cgtgtggtga cgccatgtat atctacaaag tcggagtggt tggtgcaggc
     4501 accatggggg cacagattgc ggaggtggta agttttgccg gcctgccggt ggtattggcg
     4561 gaccgagagg aagctctcgc caggcgaggc gtcgaatcgg tacgggcgat ttatcaagct
     4621 cgtgtcgcga agggcaagat gacgcccgag caattggaag aaaagatgct gctggtgacg
     4681 gcatccccga atctggagag gctccatgat gccgatcttg tcatcgaagc cgtgtctgaa
     4741 gacctgctgc tcaaacagcg agtgttccag gagctggacc ggatctgttc ccgcagcgcc
     4801 attcttgcca gcaacacttc cgcgctttcg atctcggcca ttggatccgc caccaagaga
     4861 ccggacaagg tcatcggtct gcattttttc aatccggcct atgcgatgcc cctggtggaa
     4921 gtgatccccg gtctcggcac cgatccgcag acggtcgatg atgtcgtcgg tttttcggag
     4981 agcctgcgga agcaacccgt gatcgtgaag gagtgtgcgg gcttcctcgt caatcggctg
     5041 ttgtctccct atttgaatga agccgtctgg tgcctgcagg acggcgatgt ctccatcaaa
     5101 gagatcgacc aggacatggt ttccttcggc atgcctgtcg gaccattcac tctgttggat
     5161 accgtcggct tggatattgc gctcgatgtc gcgcgcattc tgcaccgttc gtatgggccc
     5221 aggatggcgc ccgcaccact gctggaggcg ttcgtcaagg cggggcggat gggcctcaag
     5281 acggggaagg ggttgtatga ttacggcgcc gccgaggaag gtggcgccga tcaacacctc
     5341 gaacgtctga gagcgcaggc acggcagctt acgggacgaa gttcaatgaa atggtccccc
     5401 ttacgtcctc tcgttgccat ggtgaacgag gccgtcattg cgctccagga aggtgtcgcg
     5461 tccgcgcgtg acattgactt tgccatggcg gcgggaacgg gatttccgag cgacaagatc
     5521 ggcccgctcc atctggctga ccagcttggt atcgatcgcg tgcttcagga attggaggag
     5581 cttcaggaca gggtggggcc acggttctgg ccggcgccga tgttgcgtcg tttggtggat
     5641 gccgggttta cagggcaggg ggcggggaga gggtttttct cttacgaacc aaccattgct
     5701 cgaatgaggt gaggttccca tgactaccac ggccggttcg atgctcgcat gtcaccgtga
     5761 tggcgcagtg gcgaccgtga cgattcatca tcctccggcg aatacgctga cgccggaatt
     5821 gctcggcgag ctcagccagg ccttcgagat gttcgccacc gatgaggcca tcaaagtcgt
     5881 cgtgctgacg ggcacgggcc ggtttttcat tgccggggcg gacatccgtc tgttggccgc
     5941 gattgcctcc tcgcaggaag gcgcatccat cgcgcggcaa gggcaggcta tcctgaatcg
     6001 gatcgagtct ctggaaaaac cggtcatcgc ggcgatcaat ggagtctgtc tcgggggagg
     6061 aatggaattg gccctctgtt gtcacatcag actcgtggct gagggcagcc ggttgggcca
     6121 gccggaaatc aatttgggca tcatgccggg atttggcggg acgcagcggc ttccccggat
     6181 cgtcggtcaa tccaaagcgc tggaactgat tttaacgggc gatccgatct cggcgcagga
     6241 ggcgaaggcg cttggattag tctctgcggt cttcccgccg gaggatctgt tgcaccaggc
     6301 ccacagtctg gctcgcaaga tggcctccaa aagtctgccg gccttgcgtg cgtcgttgcg
     6361 ggccatccgt agtggtgcgg acatgagtct gccggaaggc ctcgccttgg aggcggttct
     6421 gttcggcggt ctctgcgaga cggaggataa gcgggaaggc ctggctgcgt ttttggagaa
     6481 acgtcagccg cggttcatcg accgttaacg actgtgcagg caaccctgcc gatgaaaggg
     6541 tgtcatggca tcggcgttcg gcaagcgggc cttggccttg ggcggaggag gcttcaccgg
     6601 ctacctcttt gagatcgggg ccttgaccgc gttggacgat ttgttcgagg acggcgtgag
     6661 tatgaacgac ctcgatctct acgtgggtgt cagcgcaggt gcggcggctg tatcgttgat
     6721 cgccaatggc gtgaagcccc gagacatcct cgaaaccaat ctgtccggga cgaggcccta
     6781 ttatttcgat catcgcaacg tgttctctcc tgccattggg gaagggctca agacgttctg
     6841 gcgcgtgacc cgccaactcg tgccgcttgt gaaatattat gtgcggcact acggtgagat
     6901 gacgctcatc gaccttctcg acaaggccca ggatgcgctc ccgagcggga tctatacgct
     6961 cgaaccattc gccctctacc tggcgggcct ctacaaaacc aaagggttga gcaattcttt
     7021 tgccggccta tcgaaggaac tctatatccc cgccattgat ctggaaacag gggagaatgt
     7081 cctgttcggc gaggaaggat ggcaggaggt gccgatttct cgcgcggtca cggcctcttc
     7141 ggcggttccc atctacttct gccccgtacg gattcaaggg cgcgattaca tcgatgcagg
     7201 aatcgggcgc atggcgtttt tcgaagtggc cgtccataag aaggtggatt tcatgatcat
     7261 gatcaatccg atggcacgcc cgcttcaacg ccggctgccg gtcgatgtcc cggaccatgg
     7321 cagcagcgtt ggtcgtttgc gggatcgcgg atttttatcg atcggtgaac aggcttcacg
     7381 catcaacttc gacgcgcgct tctctcaggc tttggaacgg ttccagcacg agtatcccgc
     7441 gaaggaagtc ctcgtgattt cgccggtgga ggaggacgcg ctcttgttcg aacggagttt
     7501 tttgagttac cgcgaccgta tccatctcct gcgcgccgga tatggctcgg tcatgacctt
     7561 ggtcagagat caattcgatc acgtatccgc acagcttgct tcatatggca tgaccatctc
     7621 tcgcgcccgc ttcgaggacc gtgctgtaca acgtctcgcg cgactgaatc acgtcgcatg
     7681 gacttcggcc ccgcgcgttg tgccgaatgc agccaccgct gcgcgtatcg gtggcatcgg
     7741 cgccgcctgg cgtaaagccc gctgactcag ggcggcagag ggcatcaatg gccaacaaag
     7801 accggatgtt cacaggatcg ctcgcggtag ccgaagccgc tcgtgacacg cgactctatt
     7861 cagggtttct ctctggtctg ttcgagggga acgtccggcg tcatctgtat tcgcgcgcgt
     7921 cgctgcctgc caggagcggc gcggcaagcg agtttctcga caggcttcgc catctgttga
     7981 ttgagaaagt ggaccccgaa accatcgatc gagagggcct gatcggagag gatgtcttcg
     8041 cggcgttgcg ggagatcggg gcgtttggca taaaaattcc acgcagatac ggcgggctcg
     8101 acttcactca gtcggaatat cacgaggcgg cgacgttgct cggcagtcat gatgcggcga
     8161 ccaccgtgtt gctgtcggcg cacaattcga tcggggcggc cgaaccggtc aagttggtcg
     8221 ggaactccga gcagcaacaa cgattgctgc cgcgactggc caggggagag gtctccggat
     8281 ttgcgctgac ggaaaagggg gcgggttgtg acatctggga tcttaaaacc tatgcgattc
     8341 ctgtccatga gcagggcctg gtggttggat atcgactaac cggcgacaaa ctgtatacga
     8401 ccaatgcgcc gcgggggcat catgcatttc tcgcgtcatg gctcgtcgtg atcgcacaga
     8461 ttgtcgactc accccaggac atccatcgcg ccaaggaaga acggcgtttc ggggcctttg
     8521 tcgtcgacac ccacacgcga gggtgctcct gcactcgcct tagctttatg ggcgttcgcg
     8581 gtatctacaa tggtcaggtg cacttgcgcg aggtgtgggt gccggtggcg gatcggctgg
     8641 gagcagaggg cgaggggctg cggcgcgccc tggaaagcct gaccgtagga cgactcacgc
     8701 tgcctgccgc ctgcctcggg aatctcaagc agtgtctctg gttggctcgt ctacgcgcgc
     8761 agcagcgtaa gcaatacgat cgcccgatcg gcgaacacac cgacatcggc gcgaagatcg
     8821 tgctcatggc ttcccgcgtg ctcgcactgg aagccattgt gaaaatcacc ggcatctggg
     8881 ccgatacgaa gcaggatgta cggttggagt ccgcggccgc aaaaatcctc gcgacggaat
     8941 ggctcctgga atcgttgctc gacctgttcc gcatctatgg cggacgtgcg tttgagaccc
     9001 cggactccct gcgtctccat ggcgacctgc cggcgccgat tgaacggatg atccgtgacg
     9061 cgctgatcaa cgtcatttgg gaaggcagca acggaatctt gaccctctgg atcggaagag
     9121 aagggctgtc acagtatttt acgcatggcc aggcgttcct cgattgtcac atcgccgaca
     9181 tggtgcgggc cacgccgttt ttcgtcaaca ccgcagcgag gtcattcggc ggtctgacgg
     9241 tatacgaaca ggactcagaa gccggctcgt tcgatccatt gtgggagcgt ttcgtggcgc
     9301 gaaagagtcg cgaactggcc cgcacgacgt tatgggttgc cgcgagagac cggcagggac
     9361 tggcccggaa gcaacggttg atgacgcgcc tggtcagtgc cgcgatgcat ctctttgccg
     9421 tcgaaacagc gctgtggtac aagtcccggc aggagcaacg cggcaaaccg catctggatc
     9481 aactggtcag ctacttctgt tcgagaacca gcgccgcgtt taatcctgag ccgttnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagtgc gtgtctgtag atcgccgggt
     9661 cagtgaagat cacgagttgc cgggagcgac atgatggaaa ttgccaggta ctttgatgcg
     9721 gtcaaggacc ggtatgactt gagcagtgat tacgcactgg ctgaaaagct ggggatcgcg
     9781 cagcccgagg cgaatttgat gcggcgcggc ctgaagatcc ccaagccgga attgtgcatc
     9841 aagatggcca agctgctcga taaaaatccg gtcgaactgc tgttgatcgc gcaaaaagac
     9901 aaggctccca aacaggccaa agaatactgg accctggccc tcacggcggt agatgtcatg
     9961 ctgcatgtgc cgaagcggcc ccggtatctg ccgaagaagg tggaagccat cggtcgggaa
    10021 ttgaaacagt tggaggcgca aactctgacg tatgaagggg cggcggcgaa cgccgaggcg
    10081 gtgcgtctga tggagacggc ggaacagtct gtcgatgcca tgatggaacg ctggaatatt
    10141 tggaagaaag gcgaagcgct ctatcccaac tatctgctgg cgaatcaagc ggcggcgcgg
    10201 cgtcacgtcc ggattcggcg gctgctgatt ctcacgcagg agcagatgca gcacgacgcc
    10261 accgtggccg atgccataca ggtgatggat gaccagcggc gggcgggcgt gaaagtgttt
    10321 tatgccttcc gggaggcgct ggttcactcg ccggctttcc agcgactgga agaagacttc
    10381 agaaaatacg gcgcggccga agacatgaat acggccatgt tcgacaggga aattttgatt
    10441 ttttcgcaga cctacgggag cgtgccgctc gggatggtcg gcacgccgac gccgattacg
    10501 atgatcaatc gtctgcagat ttcgtggaag ccggagatga ttcgagaact cgatccggcg
    10561 ccgctcttcg acatgacgcg atacgtattc gactaccagg gatccggacc gtttcacgag
    10621 cagttggcct acttcaggag gtcgatccgt gaacttccca ttcgagccgt ttaggatcaa
    10681 agtcgtcgag cccgtcaagc gcaccacccg cgaggagcgt gaccggttgc ttcgcgaagc
    10741 cgggtacaac ctcttccatg tccctgccga aagtgtcgcc atcgatctgt tgacggatag
    10801 cggaacatcg gccatgagtg attcccaatg ggccgggatg atgctcggcg atgagtcgta
    10861 tgcgggcagc aagaattacc atcacttcga aacggtcgtg cgcggattgt tcgggtaccg
    10921 ctacgtcatt cccacgcatc aggggcgggc ggccgaacat gtgttgtttt caacggttct
    10981 caaaccaggc atgtgcgtgc cgaacaacat tcattttgat acgacccggg ccaacgtcga
    11041 gcaccaggag gcggaggcga aggatctggt catcaaagag gcatacgatc cgcattgcga
    11101 actgccgttt aagggcaata tggacgtggc tcgactcgag gagaccatca agcgggtcgg
    11161 gccttcgcga atcccctttg tcatgatcac cattaccaat aacagcgccg gcggccagcc
    11221 ggtctcaatg gccaatatcc gcgcgacgag gcaggtgctg aatcgttacg gcatcccgtt
    11281 gttcttcgat gcctgccggt ttgcagagaa ttgttttttt atccgggagc gcgaaccggg
    11341 gtatgcggat agaccaattc gtgacatagc cagagaactg ttccgcctag gcgacggctg
    11401 tctcatgtca gccaaaaagg atggcctggt caacatcggt ggattcctgg ccctcaatga
    11461 tgagcgatgg gcgcaggcgg tgaccaacat gttgatcctc gtcgagggat ttcctaccta
    11521 cggcggactg gcagggcgag atctggaagc gatggcgagg gggctcgagg aagtgctcga
    11581 cgaggagtat ctccgctatc gcatcggaca ggtgcaggcg ctgggagccc tgctggaaga
    11641 gggcggcgtc ccgatcgtga agccaaccgg gggccatgct gtctatgtga atgcaaagga
    11701 attcctgccg cacctgcaac aatcccaatt cccggcgcag gcccttgctg tggcactgta
    11761 ccgggactat ggcgttcgca ccgtcgaaat cggaacggtc atgtttggca agcgggatcc
    11821 ggcgaccgag gaaacgatct acccggcatt ggaactcgtg cgattggcga tcccccgacg
    11881 agtctatacc agccagcatc tgtccttcgt ggcggaagcg ttagtggatc tctaccaacg
    11941 ccggcattcc atcggagggt tggtcatgac ttacgaggct cctcgattgc gacattttac
    12001 cgcccggttc gaggaagaga ctcttgccga gatgctatgg cccggcgcgg gattcgccgc
    12061 tgcgcctttg tcatagcggg tggggctgca tcggcatata cgtacatcat cgggccggat
    12121 agaccatgtc gatacaacga ccgactccgc gttgacctgc cttcacgtgt cccatctcag
    12181 aaaccgtgac tctcctgtcg cctgcgctgt tccaacctga cccattcatt tcccccgacc
    12241 aaaaaacttt gtggccccag gagtaatttg cgtcgagaca tgttataagc agttccttcg
    12301 caggacggat tctccaaaca cctacgtatc gccacggccg atagccgact gtcgcgcgac
    12361 tgcgagttgt ttctgaagag tgcgtgttgc agatcgcagc agcggtgtga ttcaggacaa
    12421 atatcgaaag ccgatgattt tacgggcact gattcacgaa tctgcgtgcc ccgtcatcaa
    12481 gaaagagggg aggccttgat ggcttgtgtg aagaaatcag tggggcaggc atcaggaata
    12541 tacggggcgg ctgacgcgcg tttggatgcg tccggtcgtt catccaatcg cggggatgtg
    12601 ttgcgtagat ggcgtggaac cgttgctggt gtggcggctg tgctgttact tggcgcgctg
    12661 gtcgtgcccg ctcactcaca aaccggctcg cagctcgggc cgttgcctcc gaaaccgccc
    12721 cagttacccg ttgtgcctcc gcccgcacag ccccctgcgc ttgatctccc gacccccacg
    12781 cctcctggtg cgctcgatca ggcggccaag ggacccaaga tcatggtcaa ggatatccgg
    12841 ctggtcggca ataccgcctt tacgtcgcaa caactctcgg aaatcaccgc gccatatacg
    12901 aatcgcgaat tgacggcgga agacctggaa tcgttgcgtc ttgcgctcac ctactactac
    12961 gtcaaccatg gctatttgac gtccggtgcg gtggttccgg agcaggatgt cgccgacggc
    13021 accctgacca tgcaaatcat cgagggaaag attacgcagg tcaacgtcga ggataccaag
    13081 tggttccggc cttcctattt tcagagccgc gtcaatctgg cggccggccc tcctctccag
    13141 gtcgaagcgc tccaggaacg gttgcgcgtg atgcaagcca atccccggat cgaacggatc
    13201 aatgccgaat tgttgccggg cgccacgatt ggagaaaata cgctcaatgt gaaggtgaag
    13261 gaagccaacc cgctcaaggc ctggttggag ttcaacaact atcagtctcc cgtcgtcggt
    13321 gcggaacaag gcttcatcac gttagctcac cagaacctgt tgggcttcgg cgatacgctg
    13381 agtttgcaat atggtcgctc ggccggcgtc aacccgatgc tcaactttaa atatgaaatt
    13441 ccggtgggcc ctcgcgatac gaccgtcgca ctgcaatatc gccggtttga tttcggcgtc
    13501 gaggagtcgc cgttcgattc attggatatc aagaacaagg ctcagatctt tggcctctcg
    13561 gtcaggcacc ccgtgatcag gaaggctgac caggagctgg cgttttccct cacgggcgaa
    13621 catgccagga atgagagcac gttgggcggc aatccgtttg agctcatcac cggggcgccg
    13681 aacggaagat tccgtgtgac cgcgctccgt ttcgggcagg agtatgccca acgttccgcc
    13741 gaacaggtga tttccctctt gtcgcggttt tccgtcggta tcggagcgat gggggcgacc
    13801 gccaatgctg atccgaacct gccggatgcg cggttctttt cctggttggg cgaagcccag
    13861 tggatccggc aacttccgtt ttggcgtacg cagttagtga gccgcggggt ggtgcaattg
    13921 tccaacgatc acctcttccc gcttgaacaa attgccgtcg gcggacggta tagcgtccgc
    13981 gggtaccgcg agttcacgct gattcgggat aatgccgcaa tggcgtcgat cgaagcacga
    14041 gtgccggtct atacgacaaa ggcgggtgtg gactccgtct ttctggcacc ctttttcgat
    14101 ttcggccatg gctggcaaac cactgcccag actcccggta ctccccccaa aacattggcc
    14161 agtcttggtg ctggcgtgat ctggaatttt tggcgcggga gtcattttga actctattat
    14221 ggaaaacagt tgaagcgata cgataccgat cgcaacaacc tccaggatca cggcatccat
    14281 ctgcaactgg tcgtagaggc tttttagcga gagaggggcg gatgcgatgg attcgcgcct
    14341 gtctcgctga ggcagcatgt tgtccttgag ggtaaataac cccctccacg ttgacggcgt
    14401 ttttttgccg ttggttgtgc acgtgagcca caggcgactc atgcactttg ttagggctga
    14461 atatccaggg ccgattggcc aggtctcggc ccttgtactc gcgggtccgg acagggtgtg
    14521 cgtgttgaag ttgtgtgtcg tttaaggtaa gagaggtggc ctctcttacc acatgaggag
    14581 attgaccatg ctatgtcgcc ccgcaggatt gccgctgttg gttcgtggat atctcgcctt
    14641 cctgatgctc ctcggctcta ccgcatcgat cgcttacgga caggccacca acatcgtcgc
    14701 aactccaggc ggaccgggcg gtcttggaac cacgctgaac acggcaggca atacgaccaa
    14761 cattaccggg gggacgagac cgggaggcgg tcccaatctc tttcatagct tcaatcaatt
    14821 ctcggtcgga gcgggagatg tggcgtcatt cgtgaatcct ggtggcgtgt cgaacgtcat
    14881 cagtcgagtg atcggtggat caccgtccaa catcaacggc acggtgcaag ccctcaatgc
    14941 gaatctattc tttctgaatc cttccggaat catattcggc ccatcggccc acctgaacgt
    15001 atccggatct gcgtatttca gcaccgctca gcaactacgt ctaagcgatg gaggcatctt
    15061 taccgcgagc accagcctgc tggctttcga ttcaaccttg tccgccagtt caccagtggc
    15121 gttcggattt ttaggccagg gcccttacgg gtccatcgtg ctgacctctt cttcgacagt
    15181 cctccagacc ggcgccgtct tgggtctcat gggaggaggc attcagatca acggatcaaa
    15241 gatcagcgct caacgggtca tgctcggcag tacgagttca gcgggggaaa tgagtaccca
    15301 gccatttgtt ggaaatccct tttccggggc ggctggtaat ggccaagtgc aagcgttacc
    15361 gggaacgttg atcgtcgcgg gcggtggtgg agtcattccc gcctcaattg acgtgagtcc
    15421 gaatgtcacc gtgccgacgg gtgcacaggt caacacgacg agcaattcgg tcacgcaacg
    15481 tgtgacgcaa atccaagtgg tcggcgtgaa tcttggcggc cttccgcctc tccctaccgc
    15541 aaatgcagct aacgtcaatc cgacgaatcc cgtcgaacct gtcgcgttct tgaatcgtgc
    15601 cgccatggtg cttcctcagg aagccccggc tccgccggct cctttattga ccagccggtg
    15661 cgctgcacga aaagacggca ccttcagcag tctggtgcaa gcttctcgcg acgtcacgcc
    15721 ctcgcaacca ggccggtctc tggcggcgcc tgtggtgctg gaggagatcg ccggcgatct
    15781 cgagcctggc gctccggcag caccatcagc ccacattcac ggtcagacga tcgcacaact
    15841 tatggaatca tggcaggggt gctagttgcc caaggaggac gtcatgccta cgatgcgtaa
    15901 tatggtcact cgccgcggca tgaagcggag ccatggggct gctggttcgg ccggcccgcg
    15961 taccctgtcg tctccgttga cgatactgag ccttggagtg atggcgttga gcagcttcac
    16021 ttggccagca agcgggatgg cgctggaatt gttgggcggc tctagcacgt cgtcgatcac
    16081 gccggcgcaa gaaatgcgac aggggacgac acagtttcag caaggcgcgt ttgtccaggc
    16141 ggctacgcat tggatgaatg cggcgcgggg gtacgaagaa accggacaga tcaaagagca
    16201 aagtcaggcc ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntatctggg
    16321 tggattgcgg gagcaggagc atcgcaatcc cgaggcgctg gagtatacgc gaaaagccac
    16381 gttcgccgct caaaaggtga atgcgccgga atcgctctac cgctggcagt ggcaaacggc
    16441 gagattgttg cgcgcggagg gcaaggaaga tgaatccctg gccgcgtatc agcgtgccgt
    16501 gacgctgttg aagccgattc actatgaata ttccgtcgga tatcaaggac ggcatcattc
    16561 gtactacgaa tccgtggctc cattgtttgt cgaatatgaa gatgtcttgc tgcggagagc
    16621 ggcggcggcg aagacgcccg agcaaaatga ccagttgctg gtgaaggtca aggaaacgat
    16681 tgaggtgtcg cgggcagccg agctgcagga ttatttccag gacgattgtg tcgcgacggt
    16741 ggcgagtcat cgaggcgcgg gggcgttggc gccaggcacg gcggtgatct atccgatcgc
    16801 ctttccggac cggctcgaac tgctcctgga aacgtccaac ggactcaaac aagtcagagt
    16861 tcctgtggcg ggggaaaagc tgacgaaaga aatccgttcg ttcaggcggc tgatccagga
    16921 ttcgcaatca cagaattacc tgtcttccgc gcagacgttg cacgggtggc tcgtggcacc
    16981 tattcagcag gacttgcaag gcgccggtgt ccatacactc gtggtggtgg ccgacggctc
    17041 gctcaggacc attcccatgg gagcgctcca cgatgggcgg cattttctcg tcgattcgct
    17101 cgctgtcgca gtcacgccga gtctggcgct gaccgatatg aatggagctc aacgccgcaa
    17161 gggaagtgtg ctgtcggtgg ggctgactga atcggtcgag ggccagtccg cgccgcgata
    17221 cgccgaaacc gaagtgcagg cgatcaggac gatgtatggc gggaagctgc tcatgaacaa
    17281 acagttctct acgccgacct tggaagagga gatcaaggac cagggactgg gaatcgttca
    17341 tgtggcatct catacggttg tcggcaccga ggcgagggat tcgtttgtgc tggcgcatga
    17401 tggcaagatc acgatggacc gtttgtccca gctggtggga ctccagcaat accgtcaaca
    17461 gccgctggat ctgctgacac tgagctcctg cgaaattgcc gcagaggatg atcgtgcggc
    17521 gctcgggctt accgggctcg ccgtgaagac cggagcgagg acggcgctgg caagtctgtg
    17581 gacgtcggat gatgaaacga cgacggaact ggtatcggaa ttctaccggc agttgcagga
    17641 tcccgccatt tcaaaagctg tggccttgca acgggcacag caaaagattc tttcgcagcg
    17701 cggccatacg cacccgagtt actgggcggc attcctgttg atcaacaact ggatgtgaag
    17761 ccaatccgta gaagctttga acgaggtaac aagcggacag ggtgtgaacg gtgcggagtg
    17821 gggtaggcgg ggacgttaga gtttcttccc cgtcatgatc tcataggctt cttcaatcgt
    17881 ctctacttct cggacgagga cgttcgattc cggcgtcaag ctgactcgat tgactcgagg
    17941 ggcataaaat tgccctcgtg gtacgagcag cacgtggtag ccggctctgg ccgccgcggt
    18001 tgttttttcg gcgacctgat ctacctgtcc gatccgacca tcgccttcca acgtgcccgt
    18061 gatggtgatg tcgcgtctca gctggtcacc cttcagcaga gcgatgaatc caatcgccaa
    18121 cgcggcttcc gccgttgatc cctcttcgct gagcggaccc gtgaatgtcg cgtacagcga
    18181 gagggttccg gtcgggcgaa tagaggcatc cagacgtgtg accgcaaact ggaacgcgtg
    18241 ctgaagcgac gtcatgccgg ttccgcgaac aggaacctgg ctacgccccc agcgcaatgc
    18301 taagggatcc ggctgagccc cctcgtccca tttcatcacg aacgtcggga agccgacctg
    18361 ggtgtcggta taggttgcga cagaaatggg gacgacgaga gttcgactgg cctgtgcggt
    18421 ggagggaagc agagcgaaat acccgagcag cgagaggacc aagatcgcca tcattcttga
    18481 cgctgtgccg cttctggaaa agaaagtgtg tacgcgcatg aatcaagtct agatgaggat
    18541 gcccgattgt caaattcgcc taaacggcag ggtgcgcagt cggcggatat catcaccgtg
    18601 cgtcgagcaa acaaccggaa acattgacct ctttctcttt ccgcaggcag ttccctactg
    18661 aaattcctga gggcgtcttc cctcgggccc gttgatgcct ccgaccccat gcgcgtgata
    18721 atctcgggcg agatggaaca gccggcaaga gttccggtgc ggcgcatacg ttttcaacag
    18781 tggagcaggt gagtgcaagg agccatcgtc atcaccggtg catcgactgg catcggtgca
    18841 gcctgtgcgc gttatctgga cggattgggt ttcacggtgt gggccggcgt gcgccgtaag
    18901 gaggatggcg aggccctggc tcgttccacc tccgctcgtc tgcgtgtgct gctgctcgat
    18961 gtgaccgatt ccgattcgat cactgaggca agtcgaacat tgacagacgc gttgccggaa
    19021 gcggggctgg ccggcctcgt gaataacgcc ggtatctccg tggcgggccc gcttgaactc
    19081 ttgcctatcg ccgaggtccg aacacagttc gaagtcaatg tcataggagc gctggccgtg
    19141 acacaagcct tgctcccgtt gctgcgccgg gcacgagggc gcatcgtcaa catcagttcg
    19201 atcgccgggc tggctgccac cccgtttctc ggtgcctatt gcagctccaa atttgcgctc
    19261 gaggccatga gtgatgcctt gcggctggag ctggcgccct ggggcatcgc agtggcgctg
    19321 gtcgaacctg gcgccattca atctcagata tggcagcggg ccacgatgtc cgccacccgc
    19381 acactcggcg gagtcgcgcc ggaatcgttg gccctgtatg ctcagccctt agcgcgtatg
    19441 caggatgtga tggccggggc tgccgcccgt gcgattcctg ccgaggccgt tgcccaggtg
    19501 gtggcccgtg cgttgaccgt gccatcgccg cgtgctcgtt atctggtcgg caaggatgcg
    19561 cgcttcagag cggtcctgaa gtggctgctc cccgatagcg ctcaggatcg tcttctgaca
    19621 tggtttttag gattgtcgcg tcatgcgtaa tgttgcgcac tgcggaccaa gcgggcgtca
    19681 gaaaagagag cagcggcatg gctgaaggag gactggtccg ctcggcaggc ggggtcgtct
    19741 tgcggcagca ggaagtgttg ctgatccggg tgtccgacat taaaggtcgt ccggtctggt
    19801 ccttccccaa gggccgcctg gatgccggtg aaacgccggc gcaggccgcc gtgcgggaag
    19861 tccgtgagga gacgggctgg tcttgccgca tcgatgcgga tctctcaaca accgaatatt
    19921 ggtttcaacg agagggacgt cgattccgca agacggtggt ctggttcaag atggttccgc
    19981 tcgagctgac cggcgttccg gacggagaag tggaagaagt gcagtgggtt gagcggacgg
    20041 aagccctgcg gcgactgacc tatgcgtccg atgcggcgtt attgtcgcat gcgcttgagt
    20101 tggatccttc ctctcgatag cgacaatttc tcccagtctt catctgaaca gatctgttgt
    20161 tttctcagac cacgcgtctg atgatatccg tcctccctgc cttgacaagc ctggtgccct
    20221 taactagact cgccttcaga gtggttctgc gcctcgcttt ggaaggccag agttctcgac
    20281 acgcgccttc cctcacgatt cggcatctcc cggcacccgt caatcagtgg cgcgttgatc
    20341 agtcatcccc catcgttcac ccgagggccc gcctgtgtat agtctggctc gcttttccct
    20401 gttggaaatg acggaatgct cggccgtcct gcgccagctg ggcgaacagt cgcggtcttt
    20461 gcaggaggcg gcctcgaagg cggtgaacta tcttttcgac acgctcggcg acccgaccac
    20521 ggggcaacgc gattgtgtcc ttgtccggtg cttcaaaacc atttcctaca gccggctgga
    20581 taccgaacat caacagctgg tcagagaaaa gctcggcggc atcgcgccgt tccccgattt
    20641 acgctgtttc acactcgtgg cctcaaccgg cgcggagccg gattggaatc gagtcgatcg
    20701 ttctcaccga tatcgtgtca taccgatggt cagcgccgat ttcgtgaaac aatttccgat
    20761 gttctcgcaa ctcctgcatc agttcggtgt gtccacggag ttcgacgtga ggccggatgg
    20821 tgaatggctg gtcgatctgg aggagaaaac gtataacgtg ttttatgtgc ccgacgcggt
    20881 gggaagtccc tatgtgcctg tccaggacgg atttgtcggg aaatatggtg tgaagtcggt
    20941 attgtgtttc ggggggatgt taccgtcgcg cgatttattc gcggtgattt tgttctccaa
    21001 gacgccggtg ccgcgtgaga ccgcggcgtt gtgcaaatca ctcgccctca gtttaaaaac
    21061 atccttgctc gcgtttgatg aagcgtcttg cacagacagt ggtgcgagcc agcaatcagg
    21121 atcagcgcct gagcctccgg caagtctccg tcatcagtcg cgtttgg
//
LOCUS       DAOQDS010000064        20617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_993_length_20617_cov_14.752796, whole genome shotgun sequence.
ACCESSION   DAOQDS010000064 DAOQDS010000000
VERSION     DAOQDS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20617
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_993_length_20617_cov_14.752796"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..752
                     /locus_tag="PK589_14385"
     CDS             237..752
                     /locus_tag="PK589_14385"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNJ20846.1"
                     /translation="MIFLGRRMTVRWSGEGKEQAMALIEKSIEVNVPVRTTYNQWTQF
                     EEFPKFMEGVTQVQQVDSTHLHWKAQIAGKEKEWNAVITEQRPDARIAWKSTDGAKNA
                     GVVTFHRLSDQKSKVMLQMDYEPEGVVENIGEVIGAVSLRVEGDLSRFKEFIERRGHE
                     SGAWRGEVKGK"
     gene            1133..2458
                     /locus_tag="PK589_14390"
     CDS             1133..2458
                     /locus_tag="PK589_14390"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNJ20847.1"
                     /translation="MTAFTVLLLLFLIRPVHAADYYLTATGDDRDTGLSSDHAWRTLD
                     RVNQLELLAGDRILLEAGTTFLGPLLLDGADRGTESAPITVTTFGNGPAVIEAGEGMG
                     IFVRSTAGVILSNLHVSGNHHPHNNSDGIAIHNDLDGALLLTGITIDHVEVSGFGKNG
                     ISIGGWNGDSGFRNVRVTHTRIHDNGLNGLVTYAQHPRANKDVYIAYVEAYRNTGLPG
                     VVPHSGSGIILGNVENGVIEWSKAYENGRLGNAGVGIWTYDSTKVVIQHNESFANRTS
                     GEADGGGFDLDGGVTDSVMQHNFSHDNDGAGYGLYEYAEAPPWYGNTVRHNLSVNDGR
                     KNGAAGIQIWNGGTSLLADATIHDNIVMMGPSEDVTPSAIAFKSPSQGFSIYQNAFIA
                     SGEAILVFKPAEQVHLHMSDNRCWSMTTRSLWDESLEREVCAIYFRFPG"
     gene            2668..5307
                     /locus_tag="PK589_14395"
     CDS             2668..5307
                     /locus_tag="PK589_14395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ20848.1"
                     /translation="MDSPIKVLDVDLAHPLQDLGGLDPYRSVWGLLRLNGRPVGDVTV
                     PIRDGVCTAESMYEAIVLQQGRPILQALIAHHLHDPVSRAGIRTLDPIGMDDMPANVW
                     PLVTVAVCTRDRSEGLHTCLHALQKLDYPSLDRVVIDNAPQSDRTERLVREEFPAVRY
                     VREPRPGLDWARNRALAVARGDIVAFTDDDVIVDQRWVRTLAEVFVRHPEVMAVTGLV
                     VPAELETRAQILFETYGGFGRGFEQRWYRAHSTKPQVDTFHLGAGVFGTGANMAYRRA
                     LFKNLNGFDPALDVGTVTNGGGDLEMFFRVLQEGYVLVYEPRALVRHRHRRTYQELAT
                     QLTNHGVGLYSCLSRCVRAYPALRPAGVCFGARWFWSWNIRRLLVSYVRRSSLPRDLM
                     VAELRGSLRGLFAYQTARRTARRLRDLQAGTGERAEEDMFLKLSSDLSHSPTGQPGKA
                     SCSLLPKPQFPIAVRAVELSEPLSDIGDLEKYKALQLYVTLHGELLGKHTIEHTAGIV
                     PVAQLRQAIAKSFGIRLLSRDTHHLGDAPWNRIIADLVERSPIPNPQPPKLSAGSRVS
                     IVIATLDRPEELRHCLGALSRQESSRQVEIIVVDNNPASGLTQTILKEFPAVVSVQQA
                     RRGLSYARNSGIVKSSGEIIIMIDDDVTVSSTWLERLIGHFSRPDVMAVTGNVLPLEL
                     DTEPQVLFETYGGLGRGFERLDVNGSWLRAFYSAPRTWVLGATANAAFRASVFRDEHI
                     GLLDEALGAGMAGVGEDTYLFYRILRANHRLVYEPSAYVWHKHRRDIRAFRRQLFAYS
                     KGHVAYHLTTLFRDGDLRVLLYLFVSLPRAYLFRAKSRLLGRTVYPLSLLLLEILGNL
                     IGPWTYVACRWKASRHSAERVSR"
     gene            5304..7307
                     /locus_tag="PK589_14400"
     CDS             5304..7307
                     /locus_tag="PK589_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20849.1"
                     /translation="MTAHNIPAVDSLPHREEPSTDQISADFRMLLGAVDVVSFDLFDT
                     LIQRDALFCPKDLFYRVQAVVEHRHAITLKDFVSTRVRAESIARVRARGRQSEETTLE
                     DIYVEMANILSLPSSFAEEMLHIELECERAALMALPDGKRLYDAAIRAGKTTVVISDT
                     YLSRVFLEEILMKAGFNGVHRVYASSEFGKTKHHGALFGIVLKDLKCSPGRLLHVGDN
                     PLSDATAALGQGLRSFICTNPKQRLKWRYGLSDRPSGSQAISALLCDISRTAEEQSHG
                     TTDRAVIARSAVEHLSWVYEGFAAWLVHQMASQRDTRVYFASRDGLIMKKFFDVTRKA
                     FGTPIESRYLYVSRAALYPMLVLTDPDMARRLFCHSWDHLTMTQALKRMSLVPQEVAG
                     QLAKYGLADPLLSLNRVTTLKLSRFLDAIWPLLCAKSESHCELVAEYLRQEGFLADEP
                     TAFVDIGWHASLQHCLVKLLQHRGFKNTLHGYYLGTFARPDASPEVSAKGFLIENDAP
                     ASISALIRTGPSLLELFHAAGHGSVLGYERKDGRVVPVLEENEREQEQFRRIIEPLQQ
                     HAFEFVAARLARGDKPEASTFPPELVARLGLRILHKPTDEEAEIFGRLRIATDFGGSM
                     KSLTGACEWNLSEVVGDALPDGIPPMWYPGFYALKKSATKRAS"
     gene            7319..8851
                     /locus_tag="PK589_14405"
     CDS             7319..8851
                     /locus_tag="PK589_14405"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional class I SAM-dependent
                     methyltransferase/glycosyltransferase family 2 protein"
                     /protein_id="HNJ20850.1"
                     /translation="MDDLRDTRIDTLALREKYRDQYFLHRDPIYQDRLLWRAHTFRHI
                     MHLLPTQSILELGCGRGLFTPHLCRVSRGENPVTSVTFRAQQARPDSLSADVEFLYIS
                     ALPGPLRGKHYDFIVAIDLLDQRNCGWFLQQIYELLRPGGQVLFYESNPWNLALRAKH
                     CWRRLRGGDDPRRLLNRPVLYELMSEVGFIRVFALYNDFVYAPLTRSLIWFLRNMSIL
                     LENARGIQTFAGSILLHAQKPPRTTVRPLRSLCEHAQLHRAISVVIPCHNEEMNVEPL
                     VTQLMALYGDYIHEIIPVDDNSTDRTAEVLRRLAGAEPRVKPVFRTPPNGVGRALADG
                     YRVATGQYVLSMDCDFQHLLPEIRDLFDAAAQGYDVVIGSRFSRHSVLLNYPFKKILA
                     NRAFHALGQILFLRQFRDLTNNLKLMRREVVERLQLTQPHFAVNAETGLQPLLMGYSL
                     REVPISWINRTPDMGSSSFHLGKVGRGYMAVLFRLWWKHLAGAIAVPLKMKSSNSESS
                     LR"
     gene            8857..11046
                     /locus_tag="PK589_14410"
     CDS             8857..11046
                     /locus_tag="PK589_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20851.1"
                     /translation="MAVTHDEPASMLKQEDFVFTNSSLSQEPANTPVWRSDRSVRVFV
                     LSIWLGMVMLAMVNWAEGSRNVPFSEDWLLVSALMGHEPYLGTWLWSQHNEHRVPVPR
                     LILLALLTVGQGDFRIGGAFNILALGGVSLLMMETARSVRGGSSRVADAFFPVAFLHL
                     GHTENMLWTWQITQVLPVILVSALMLIVVAGRIPRTTASTLAAGVCTIMLPLCGANGL
                     LYAPLFAFWIGYVGIVIIAGRSNEHIAGENWKGRYLVGAAAVTLLLSGLYFVQYHPPQ
                     YGAEAPTLPGALYATLQFIALSVGPAARMSWVLSIAAVMVCLVPALVIGARHFVARQD
                     RNPFRPVGVVVCAANLLLSALAIGWGRAQVLSLWGGLWPIRYSLFAVPILCLAYFVYE
                     LYGSTWTRQAFQTLLFVGMVLLVPANATQGARWHDWYLQGAGAFERDVQSGASVSRVG
                     ERNREYLYRWMDPSFDKVRMLRDSGIGPFAKMAEEPSAPVQAPRHQGMPESPVAESLP
                     AARRELRFVMPEAAEVFLVWGINGWQAAPESFRPAGTVVKDHVLHTPMHRQDGVFVMP
                     LALPRGASLDYCFLITKKREAFDITWPLCEGNYHEEFMADGVREIRSRTSLALVRQEI
                     RYHAPEAKEVHLVWGVKGWHVTPQSLWPSGTAIIDHVMHTPMALREGIFVATVTVPVD
                     TAVDYGFQITKRRGIFDLVYPLFDGNYSVRPSQDGQIDMQAKPGLIP"
     gene            11134..14844
                     /locus_tag="PK589_14415"
     CDS             11134..14844
                     /locus_tag="PK589_14415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ20852.1"
                     /translation="MNDSPIAEPSAKTMVFIVPSLSRSSAQDEDVVQLLLCAAARVEQ
                     LLSASPGLVVKATILSVDQDLSGWVQVGALFSDRISKHVTVEPLKEPATPCLAIPAHK
                     SVYHVHEFLRERPFDEVHCLDRHGYVYYSAHAKRLGLFFLTTLFCVHVVGGTIFRREC
                     QDLLLDDPDILAIDLLERGTVERADLHYIHDIKAWEWYDEKVVLHAGSTSYDVTWPVD
                     PPTSVVVEGNESSELPALIYCGPLGADGGLPLFCDVVDRLVSQGERLKHVTFVGAAHA
                     IGGVDAVTYIRLRSSGWHLPITIKRDLSLADEMTFLAGLQGVVVCHTARRNHLRSRLI
                     VGGLQNVLHIRAVNESRDGARSGGVLPGQITQTVTRLLRAPLQQSPVSLPPLTQLWKA
                     GRKLPDHEGVRSSLPLGVPNEPQPRVSVCITHFSRPQKLRMALQSLKQQTYRNFDVIV
                     VDDGSPDPDVQKELALIGDEIRPLGWRILFQENRYLGAARNVGARHATGEFLLFMDDD
                     NVAKPHELSTFVAMAQRTGADIITSFFDVFETEATLESDGPPPLRFTPYGADPALGIF
                     TNCYGDANALYSRRAFDKLGGFTEDFGVTHEDWELFCRASLEGVTLLCIPEPLFWYRM
                     DQSAMFRGSRTQLHKSTNLRRHIRPYLEKLPRYQSNLVQLLQGLSTTLPLTTVGTVSR
                     NAAPLALRQPDATLPYGRVAVITRTKDRPLLLHRAIRSVLSQTFKDWVLVIVNDGGDP
                     QRLEKVVDEVREELADRILVVHHPMPLGMQAASNSGIAHCDSDFVIIHDDDDSWDPTF
                     LARTVSYLDDHGWNARLGGVITWSWVIIEEVADDGAVKVHNRFVFNDKLHNVALIDMT
                     VENRFPPISFLFRRAAMDHVGPFRSQHGPLGDWEFHLRLLQHFDVDVIPDPLANYHHR
                     TNTTIGIYGNSVHAHKDQHQASRIHLLNEALRRDLDSGQGLSLAQLLTLGELHHSSKA
                     EQAREFQRLHDYLWNIDQRVQAIATQVHSSKHGLRAKSRRNLVSNGDFRQWPGLGPNQ
                     FGPHETYTFLDVSPGCVLCYDGRQVSYRLEQRKWTTDGQQLAFGKTFLHIENDGYTKG
                     GSWFAIEFMIPSALLLSGHTITISGVSRLNALQGWIGLGGRYLFGDGRQLDWPDHRID
                     VASDFARWTCTVMCPAIQETEVGHGHQSRILLKLPHNQPFDFDLTNLQVELGSESSDF
                     EYSSGKVSLRHRVHLVLRGLKRRLRAAKRAIG"
     gene            14885..15640
                     /locus_tag="PK589_14420"
     CDS             14885..15640
                     /locus_tag="PK589_14420"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ20853.1"
                     /translation="MSGISWNLIRHLVARDFRIRYSGSVLGVVWSVLIPMAQLGVLVF
                     TFGSVVPVNIDDYPAFVYSALLPWTWFSACLGSTGSLFFSHRDLVRRPNFQPIILTVV
                     NTLSNLVTFLLSLPLLFLLLAWYQRSVAASLVALPALLVLQAVMTVGLSLLVSTWNVF
                     YRDVSQLVNIVLSLLFFLTPIFYRPVVESKYAMIFQFNPLAVLISSYRNILFEGRLPE
                     WDAWAHITAVSAALLVCGYLVYRHELPDVVDTV"
     gene            15650..16906
                     /locus_tag="PK589_14425"
     CDS             15650..16906
                     /locus_tag="PK589_14425"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ20854.1"
                     /translation="MHAAMTVTDLSKRYRISRHKVTTLRETVDRWIKGRQDVTRTMWA
                     LRDVSFTIEQGRVFGIIGHNGAGKSTLLRLLCGLGRPTSGRIEAGGYVSGILELGGGF
                     HAELTGRQNLMTVGILNGLTPAEVKAREQDIIAFSELEDFIDQAVRTYSNGMYLRLAF
                     AAAIEFDPAILIIDEVLSVGDERFQQKCLDRIAAFRASGKTLVITSHDTDQIQRLCDE
                     VLVLEEGRAIMQGDPKSAIACYHDLMRQRTERRAAHIGGGSTRSLTTASGTRQGTQEA
                     TIAVVRLCRSDGQAVETIRSGEGILAELEIDAPPSIEDFALTVGVFNEGHVKCFEATI
                     ASVQGTLGKVSPHSSIQCHLKHLPLLSGSYFINVGIYPTDWSYVYDFHWQMHPVTVFD
                     ERTSNSQAGGIVAVETHWTIISRGDV"
     gene            16903..18810
                     /locus_tag="PK589_14430"
     CDS             16903..18810
                     /locus_tag="PK589_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20855.1"
                     /translation="MRPAIRAAGLVCCCVIMPLWHVVQVGAETTTAPASPPRMLTREI
                     RYHMPEAGEVWLVWGVNGWHRLPEAAWPPGTVLGKTGLMTTRMIEQGDAFVATVAVPN
                     GTALDYCFLLTKKREAFDITWPLCEGNYSEVATRSGVTEIGSRLSLAVVTQEFHYHMP
                     DAQEVSMVWGVNGWSPVPESLRPLNTKMSDNLMVSPMRKREQEFIVSVQVPVGATIDF
                     GFRITRSAMGSVNIWEGGGAEGFHEPVRGGSSIRVKSNAIILPRGRPSRDEDNGWHVA
                     WAIVGAVSAPLLLAGLLVMLRRRQARQRSANPFNPRREKPSSALDGLLILAAVGIGAF
                     GAEQMLRNVYPHEGFGIAAEDEWFRQAPYSANQLFTIDPTLGIRPWLRTGRYTEYGTV
                     PNMYRLAKDSRHTRILFVGNTVTYEGTLLHALRELHGEDRFEYWNAGVPTFNTVQQIA
                     YYHAYNARTAPDHVILTIQPDDLGTIPIVFRNHTAELEVHRPAIPRKDLDTELYGSSY
                     LYRYVKGLLISPEEKLHRLWQEYRDSVKSFSERLAHDHIRLSVVVLPVLASQQEWTQA
                     ERDRRQALFSVLTELQIEHYDLAQAFEPGRAENAVLPPMSKESWAPSSEMVSAFAQYM
                     THEGLFRTKEH"
     gene            18812..19813
                     /locus_tag="PK589_14435"
     CDS             18812..19813
                     /locus_tag="PK589_14435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ20856.1"
                     /translation="MSPHHTHRHENRARSNHADESTHDVQRGDSSESISGLVSIVIPC
                     YQQAHFLPDAISSILRQTYPDIEIIVVDDGSTDHTRDVALSHPGIRYIAQKNAGVSSA
                     RNTGFRMSRGEYIVFLDADDRLIADAIEIGTRSLAEHPACALTFGTFFMIDAEGTRHN
                     PSYPLNAVTYGYKELLEENAIGNPGVALYRRWALSVVGGFDHRNGPAGDYDLYLRIAR
                     LFPVACHRRPVIEYRRHGGNMSNDPGVMLKACLSALNKQRAFIKNQPDLTLAMRRGRI
                     GWKLYYGEPLVSKVQDEFSNRNWKEGLRGLLQLAIWYPERFLTGLSRKATKLKNRMR"
     gene            19845..20474
                     /locus_tag="PK589_14440"
     CDS             19845..20474
                     /locus_tag="PK589_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20857.1"
                     /translation="MKSCPPKSAWRSRLALMVVLCGLPGCVTDAPTLTESPDEGRKVL
                     LMGNVVTVLTGDRARKFEPAVRDIELIEQNAGTRYRVQVDGPDKPFAVFVPPGRYDIT
                     RVKIHEGPFLSIAHLTASFSVEEGSPIFVGTWRFGVDSPRYGRMVAVSMVLDDEARQQ
                     AEQELRETYPDLVSPRMLTVLPAPSELQTRLYEVAPYPRISRYFRRHYW"
BASE COUNT         4248 a         5684 c         6073 g         4612 t
ORIGIN      
        1 tgaagcgcgt tgggaactga atcccgcctc ttcggaagat tttgaggagc ggcaatcgga
       61 actgcgcgga aagatgccct ctcgtcgcat acgaaacggc gaggaaaatt cctgaacagg
      121 agagagctgg taggacaatg ctttcgcctt cgcgagaacc aaaaacgagg tcctagggaa
      181 gtccctagtt ataaccaacg aggtaaaatg cctaaatgac gtcatgagga gtcgagatga
      241 tctttctcgg tcgtcgcatg acagtgcgat ggtcgggcga gggaaaggaa caagctatgg
      301 ccctcatcga aaaatccatc gaggtcaatg tcccggttcg aacgacatac aatcaatgga
      361 cgcagtttga ggaattcccg aagtttatgg aaggggtcac gcaggttcaa caagttgata
      421 gcacgcatct gcactggaag gcacagattg ccggcaaaga gaaagaatgg aatgcggtca
      481 ttacggagca acgtccagac gcacggatcg cctggaaaag tacggacggc gcgaaaaatg
      541 ccggcgtcgt gacgttccac cgcctgtctg atcagaaaag caaggttatg ctgcagatgg
      601 actatgagcc ggaaggggtc gtcgagaaca tcggtgaggt tatcggggcc gtgtcgctcc
      661 gcgtcgaagg agatttaagt cgctttaagg aattcattga aaggcggggg cacgaaagcg
      721 gcgcctggag aggtgaggtc aaggggaagt aatgcccggg cattgcgcaa gtggatcaga
      781 ccatacatgt aatgggaaga cgctgctaca agtattcttg tgctcctacc taagacgcgc
      841 ttcctgactg ccggttttcc ggctcaggat ccgcagatgc aagctcactc cgtgctgttc
      901 tcaggcctag attaccggcg atttcgcgag tccggtggag gcaatctatt ggccgaggtg
      961 tctggtgcct ttgcgacaga atggcttgca caacaactga tggctgccac agggagcgag
     1021 agtggttcaa acaggcaatg cccgaagcga tcgtgtcgtg ggacagtctc ctcgctcata
     1081 tccagggagg tatggtcctc ggaatgagga ggcgttcccg ctactcagag taatgaccgc
     1141 gttcactgta ttgttgctgc tctttctcat tcgtcccgtc catgctgcag actattacct
     1201 gaccgctaca ggcgatgatc gcgacaccgg tctctcttcc gaccatgcct ggagaacgct
     1261 ggatcgcgtc aaccagcttg agctcctagc aggagatcgc atcctcctcg aagcgggaac
     1321 tacgttcctc ggccctttgc tgttggatgg ggcagatcgg ggaacagaat ctgctccgat
     1381 caccgtcaca acatttggta atgggcctgc cgtcatcgag gctggcgaag gcatgggcat
     1441 ctttgtacgg agcactgcgg gggtcattct ctcgaacctg catgtgtcgg gaaatcacca
     1501 tcctcataac aattcagatg gcatcgcgat tcataacgat cttgatggag ccctcctgct
     1561 gaccggcatc accatcgacc atgtggaggt gagtggattt ggcaagaatg gaatctcaat
     1621 aggcggatgg aacggcgaca gcggctttcg gaatgtacgg gtcacgcaca cacgcattca
     1681 cgacaatggc ctcaatggcc tggttaccta tgcgcaacat cctcgtgcca ataaagacgt
     1741 ctacatcgcc tatgtcgagg cctatcgaaa cacggggttg ccaggcgtcg tccctcacag
     1801 tggcagcgga atcattctcg gcaatgtgga gaatggggtg atcgagtggt caaaggccta
     1861 cgagaacggg cggttgggga atgccggtgt cggaatctgg acgtacgatt cgacgaaagt
     1921 cgtgatccaa cacaatgaat cgtttgcgaa ccgtacctcc ggcgaggcgg atggcggcgg
     1981 attcgatttg gatgggggag tgactgactc cgtgatgcag cataactttt ctcacgataa
     2041 tgatggtgca ggctatggac tgtatgaata tgccgaggct cctccctggt atggcaatac
     2101 cgtacggcac aacctcagcg tcaatgatgg tcgcaagaac ggtgctgccg gcattcagat
     2161 ttggaatggc gggacaagtc tcttagccga tgcgaccatt cacgacaata ttgtgatgat
     2221 ggggccatcc gaagatgtca cgccgagcgc aatcgcgttt aagtctccca gtcagggatt
     2281 ctcgatctat cagaatgcgt ttatcgcgag tggtgaagcg attctggtgt tcaaaccggc
     2341 ggagcaagtc catctccaca tgtcggataa ccggtgctgg tcgatgacca cgcgatcctt
     2401 gtgggacgag tctctcgaaa gagaggtgtg tgccatttac tttcgtttcc ctggatgaag
     2461 gtgtttacca ggattgcggt cactgcacgg ttagacgtct tccattccgg agtgtgtgag
     2521 tgactcgtgc gttactccta ccatcctcaa ccgagagggc aggcatgttc gcacgagtct
     2581 gccgattgtt tccgttcaga tcacaacggc gtgacaagtc accgtcttgt gccgagacgt
     2641 atagaagaaa gtgaagcgta atcgaccgtg gacagtccca tcaaagttct cgatgtcgat
     2701 cttgcccatc cactccagga cctgggcggc ctcgatccct acaggtcggt gtggggactt
     2761 ctacgattga acggtcgacc ggtcggagac gtcactgtgc ctattcgaga tggagtttgc
     2821 actgccgaat caatgtacga agccatcgtg cttcaacagg gacggcctat tctccaagcg
     2881 cttatcgccc atcacctgca tgaccccgtg tcgcgcgcgg gcattcgcac ccttgacccg
     2941 atcgggatgg atgacatgcc cgcgaatgtc tggccgctcg tcacagtcgc ggtgtgtacg
     3001 cgggatcgtt ctgagggatt gcatacctgc ctgcatgctc ttcagaagct ggactatccc
     3061 tcacttgacc gggtcgtgat cgataatgcc ccacaaagcg acagaaccga gcgcctggtg
     3121 cgcgaggaat ttccagcagt gcggtacgtt cgagagccga ggccgggtct cgattgggca
     3181 agaaatcggg cgctggcggt ggcacgtggc gacatcgtgg cgttcacgga tgacgatgtc
     3241 attgtcgacc agcgatgggt gcgcaccctc gcggaagtgt ttgtccgtca tccggaggtg
     3301 atggcggtaa ccgggttagt ggtgcccgct gaattggaaa cccgggcgca aatactgttt
     3361 gagacgtatg ggggattcgg ccgcgggttc gagcaacggt ggtatcgagc ccattctaca
     3421 aagccgcagg tcgatacgtt tcatcttggc gctggtgtct tcgggaccgg cgccaacatg
     3481 gcgtatcgtc gtgccctgtt caaaaatctg aacgggttcg atccagctct cgatgtgggg
     3541 acggtcacca atggtggcgg tgatctcgag atgttttttc gcgtgctaca ggagggctac
     3601 gtcctcgtct atgaacctcg agccctcgtc cgccaccggc ataggcgaac gtatcaagaa
     3661 ttagcgacgc agctcaccaa tcacggcgtg gggttgtact cctgtttgtc ccgatgcgtt
     3721 cgtgcctatc ccgccctccg tcccgcgggg gtgtgttttg gcgcgagatg gttctggtca
     3781 tggaatattc gccgactcct cgtgagctac gtgcgccgtt cgtcattgcc tcgcgacttg
     3841 atggtggccg aactgcgcgg atcgctccgc ggtctctttg catatcagac ggctcgacgc
     3901 acagcgcgta ggctgcgcga tctgcaggca ggtacaggtg aacgcgccga agaggacatg
     3961 ttcctcaagc tctcgtcaga cctctcgcat tcgccgactg ggcagccggg caaagcgtct
     4021 tgcagtctgc tgccgaagcc gcaatttccc atcgccgtac gtgccgtgga actcagtgag
     4081 ccattgagcg atatcggtga tctggagaaa tacaaagcgc tgcagctgta cgtgaccctg
     4141 cacggtgagc tcctcgggaa acacaccatt gagcatactg cggggatcgt gccggtcgct
     4201 caactccgcc aagccatcgc caaatcgttt ggcatacgcc tgctctcacg agacacacac
     4261 catctcggcg acgcaccctg gaacagaatc atcgcagacc tggttgagcg gagtccgatt
     4321 ccgaatccgc agcctcccaa actgtcagcc ggcagccggg tttccattgt gatcgccacg
     4381 ctcgaccgtc cggaagagct tcgacactgt ctcggcgcac tgtcacgcca ggagtcttca
     4441 cggcaagtcg aaattattgt ggtcgacaat aacccggcgt ccggtctcac gcagacgatt
     4501 ctgaaggagt ttcccgcggt ggtttcggtg cagcaggctc gcaggggact cagttacgcg
     4561 agaaattcgg gcattgtgaa aagctccggc gagatcatca tcatgattga tgacgatgtc
     4621 acggtgtctt cgacgtggct ggaacggttg atcggccatt tttctcggcc agacgtcatg
     4681 gcggtgaccg gcaatgtgct tcctctggaa ctcgacacgg agccgcaggt gttgtttgag
     4741 acctatggag gattggggcg agggtttgaa cggttggatg tgaacggaag ctggctgcgt
     4801 gcgttttact cggctccgag aacctgggtg ttgggtgcaa cggccaatgc cgcatttcga
     4861 gccagcgtct ttcgtgacga gcacatcggg ttgctggacg aagcgctcgg cgccgggatg
     4921 gctggagtcg gagaagacac gtacctgttt taccgcatac ttcgagccaa ccacagattg
     4981 gtgtatgaac catcggccta tgtctggcac aagcaccgac gagacatccg tgcctttcgt
     5041 cgtcaactgt ttgcctacag caaagggcat gtggcctacc atctcacgac gctgttccgg
     5101 gatggggatt tgcgcgtcct gctctatctc ttcgtgtcgc ttccccgggc ctatctgttc
     5161 agggcgaagt ccagactcct ggggcgaact gtgtatccgt tgtctttgct gctgctcgaa
     5221 atcctcggca atctgatcgg tccctggaca tacgtcgcgt gcaggtggaa agccagccgg
     5281 cactcagccg agagggtctc acgatgaccg ctcacaacat cccggcggtc gatagcctgc
     5341 ctcaccgcga ggagccttcc acggaccaga tatctgccga tttccgcatg ctcctcgggg
     5401 cggttgatgt ggtgtcgttt gatctgttcg acacgctcat tcaacgggat gccctgttct
     5461 gtcccaaaga tctcttctat cgggttcagg ccgtcgtcga acatcgccat gccatcacac
     5521 tcaaagattt cgtttctacc cgagtacggg cggagagtat cgcccgtgtt cgagcgcgag
     5581 gacggcagag tgaggaaacg acgctcgaag acatctacgt cgaaatggcc aatatcctga
     5641 gtctgcccag cagtttcgct gaggagatgc tgcacataga acttgagtgc gaacgggcag
     5701 ccctgatggc cttgccggat ggaaagcggc tctacgacgc cgcaatccgc gcaggcaaaa
     5761 ccaccgtggt gatatcggac acctatttga gccgggtgtt tctcgaagag atcctcatga
     5821 aggctggatt caacggggtg catcgcgtgt atgcctcctc ggaattcgga aagaccaagc
     5881 accacggcgc gctcttcggc atcgtgctga aagatttgaa gtgttcgccg ggccggctgc
     5941 ttcacgtcgg cgataacccc ctgtccgacg ccactgccgc gctgggtcaa ggactccgaa
     6001 gcttcatctg caccaacccg aaacaacggc tgaagtggcg ctatgggctc agcgacagac
     6061 ccagcgggag tcaggccatt tcggcgctgt tgtgcgacat cagccgaact gcagaggagc
     6121 aatctcacgg cacgaccgac cgtgccgtga ttgctcggag tgccgtggag catctctcct
     6181 gggtgtatga ggggttcgcc gcctggttag tgcatcagat ggcgagtcag agggatacac
     6241 gagtctactt cgcctctcgt gacggcctta ttatgaaaaa gttcttcgac gtcacgcgaa
     6301 aggccttcgg gaccccgatt gagagtcgct acctctatgt ctcacgggca gcgctctatc
     6361 cgatgctggt tctcaccgat cccgacatgg cgcgacggtt gttctgccat agctgggacc
     6421 acctgacgat gacacaggcc ttaaagcgga tgtccttggt tcctcaggaa gttgccgggc
     6481 aactggccaa atatggcctg gcggatcctc tgctctcgtt gaatcgagtc accacattga
     6541 agctgagccg gttcctcgat gccatctggc cgttactctg cgcaaaaagt gagagtcatt
     6601 gcgaactcgt ggcggagtac cttcgacagg agggattctt ggccgacgag cctacggcat
     6661 ttgtcgatat cggatggcac gcgtcgctac agcactgcct tgtgaagctg ctgcagcatc
     6721 gcggtttcaa gaatactctt cacggctatt atctggggac ctttgccagg ccggacgcat
     6781 ccccggaggt ctctgcgaaa ggcttcttga tcgagaatga tgcgcccgca tcgatctccg
     6841 ccctgatccg gaccggtcct tccctgttgg aactattcca tgccgccggc catggatccg
     6901 tgctgggata cgagcgtaaa gacggtcgcg tcgtacccgt cctggaagag aacgagcgcg
     6961 agcaggaaca attccgcagg atcatcgaac cgttacaaca gcacgccttt gagtttgtcg
     7021 cggcgcgcct cgcccgagga gataagccgg aggcctcgac gtttccgcct gaactggtcg
     7081 cccgcttggg gttacgcatc ctccacaagc caaccgatga ggaagctgaa attttcggac
     7141 gactgagaat cgccaccgat tttggcggca gcatgaagag tctcacgggc gcttgcgaat
     7201 ggaacctctc ggaggtggtg ggagatgcgc ttcctgacgg catccctccc atgtggtatc
     7261 cgggatttta tgcgctaaaa aaatcggcca ctaagcgagc ctcgtaggga ccattgtgat
     7321 ggacgacctt cgcgatactc gcattgacac attggcgctc agggaaaaat accgcgatca
     7381 atactttctg catcgcgatc ccatttatca agaccgtttg ttatggcgag cgcatacgtt
     7441 ccggcacatc atgcatctcc tgcccactca atcaattttg gaactgggct gtgggcgcgg
     7501 attgttcacg ccccatctct gtcgtgtgtc gaggggagag aatcccgtca cgtccgtcac
     7561 gttccgggct caacaggctc gccccgattc tctctctgcc gacgttgagt ttctttacat
     7621 ctccgcgctt cccggtccgc tacgggggaa acactatgat ttcatcgtgg ccatagattt
     7681 actggatcaa cgtaactgtg gatggttctt gcagcagatc tatgagttgt tgcggccggg
     7741 tggccaagtc cttttttatg aaagcaaccc ttggaacctg gcgttgcgag caaaacactg
     7801 ctggcgccgc ctgcgcggcg gcgacgatcc gcgtcgcctg ttgaatcgcc cggttctcta
     7861 cgaattgatg tccgaagtcg gattcattcg cgtattcgcc ctgtacaacg atttcgtata
     7921 tgccccgtta acccgttcgc tgatctggtt tctacgcaac atgtcgattc tccttgagaa
     7981 cgccaggggc atccaaacct ttgccggctc tatcctcctg cacgcccaga agccgccacg
     8041 cactaccgtc agaccgctgc gttccctgtg cgaacatgcg caactccatc gtgcgatctc
     8101 ggtcgtcatc ccttgccata acgaggagat gaatgttgag ccgctggtta cgcaactgat
     8161 ggcgctctac ggggactata tccatgaaat tattccggtt gatgataaca gcacggatcg
     8221 tactgccgag gtactgcgcc gcctggccgg cgccgagcca cgcgtcaaac cggtttttcg
     8281 gactccgccg aacggggtgg gacgagcgct ggccgacggc taccgagtag cgacagggca
     8341 gtacgtcctg tctatggact gcgattttca acatctcctg cctgagatca gggatctgtt
     8401 cgatgcggca gcacaggggt acgacgtggt gatcggcagt cgtttttcgc gccatagcgt
     8461 gttgctcaac tacccgttca aaaaaatcct ggctaatcga gcgtttcatg cccttgggca
     8521 aatcctgttt ttacgtcagt tccgggatct gaccaacaat ttgaaattga tgcggcggga
     8581 ggtggtcgag cgccttcaat taacacagcc gcattttgcc gtgaatgccg aaaccggtct
     8641 gcaaccgttg ctgatgggct attcactccg ggaggtgccg atctcatgga tcaaccggac
     8701 acccgatatg gggagctctt cctttcatct tggcaaggta ggccggggct acatggcggt
     8761 cttgttccgg ttgtggtgga aacatcttgc aggagcgatc gcggtccctc tcaagatgaa
     8821 atcgtcaaac tctgaatcga gcttgagata gcggccatgg cggtcacgca tgacgaaccg
     8881 gcaagcatgc tgaagcagga ggatttcgtc ttcacgaatt cgtcactcag ccaagagcct
     8941 gccaatactc cagtctggcg atcggacagg tccgtgcggg tgttcgtgtt gagcatctgg
     9001 ctggggatgg tgatgctcgc gatggtcaat tgggccgaag gcagtcgaaa cgtgccgttt
     9061 agtgaggatt ggttactggt ctcggcgctc atgggccatg aaccgtatct cggcacatgg
     9121 ctgtggtcac aacataacga acatcgtgtt cccgtgccgc ggctcattct cctcgcgctc
     9181 ctgacagtgg gacagggtga ttttcggata ggaggagcct ttaacattct cgccctcggc
     9241 ggagtgtcgc tgttgatgat ggaaacggcg agatccgttc gcggtggctc gtcgcgagtc
     9301 gcggacgcgt tcttccccgt ggcgttcctt catcttgggc atacagagaa tatgctgtgg
     9361 acctggcaaa tcacgcaggt tcttcccgtc atcttggtgt ccgccctcat gctcatcgtg
     9421 gtcgccgggc ggattcctcg gaccaccgca tcaacacttg ccgccggtgt ctgcaccatc
     9481 atgttgccgc tctgcggggc aaacgggttg ctgtatgcgc cgctgtttgc cttctggatc
     9541 ggttacgtgg ggatcgtgat catagccggt cgctcgaatg aacacatcgc cggggaaaac
     9601 tggaaaggtc ggtacttggt cggcgccgct gctgtgacgc tactcctgtc cggactctat
     9661 ttcgtgcagt atcacccgcc acagtatggc gccgaagcgc cgaccctgcc gggcgcgctc
     9721 tacgcaacgc tgcaatttat cgccttaagt gtcggacccg ccgcaagaat gtcctgggta
     9781 ctctcgattg ctgcggtgat ggtgtgtctt gtgcctgccc ttgtcattgg cgcgaggcac
     9841 ttcgtcgcac ggcaggacag gaacccattt cggcctgtgg gagtggtcgt ctgcgcggcc
     9901 aatctcctgc tgtccgcctt ggctattggc tggggccgcg cgcaagttct ttcattgtgg
     9961 ggagggctct ggcccatccg ctattccctg ttcgccgttc ccattctgtg tttggcgtac
    10021 tttgtctacg agctctatgg atccacttgg acgcgtcagg cgtttcaaac gttgttattc
    10081 gtcggcatgg tccttctcgt accggcgaat gcgacgcaag gtgcacgatg gcacgactgg
    10141 tatctgcagg gtgccggggc attcgagcga gatgtgcaat cgggtgcatc ggttagccgg
    10201 gtaggcgaaa ggaatcgtga atacttatat cgatggatgg accccagttt cgacaaagtg
    10261 aggatgcttc gagacagtgg gatcgggcca tttgcgaaaa tggccgagga gccgtctgct
    10321 ccagtccaag cgcctcgtca ccagggaatg cccgagtctc ccgtagcaga gtctctgcct
    10381 gcggcgagga gagagttgcg ctttgttatg cccgaagcgg ccgaggtgtt tctcgtgtgg
    10441 ggcatcaacg gctggcaagc cgcccctgaa tcgtttcgtc ccgccggaac cgtggtgaaa
    10501 gaccacgtgt tgcacacgcc catgcatcga caagacggag tctttgttat gccgctggcc
    10561 cttcctcgag gcgcgtcgct cgactattgc ttcctcatca cgaagaagcg ggaggcgttc
    10621 gatattacct ggcctctgtg tgaagggaat taccatgagg agttcatggc agacggagtg
    10681 agggagatcc gttcacgcac gagccttgcg ctggtgagac aggaaattcg ctaccacgcg
    10741 ccggaggcca aagaagtgca tctggtgtgg ggcgtgaaag ggtggcatgt cactccccaa
    10801 tcactctggc cttccggcac ggccattatc gatcacgtca tgcacacacc tatggctcta
    10861 cgagagggga tattcgtcgc caccgtgact gttccggtcg ataccgcggt cgattacggg
    10921 tttcagatta caaaacgtcg aggcattttt gatttggtgt accccctgtt cgatggaaat
    10981 tacagcgtgc gcccgagtca agacggacag atcgacatgc aggcaaagcc cggactcata
    11041 ccgtgatgga tccggaggca tgggtaggga agaggttgcg agtcgcagta tttatgcagg
    11101 aacgagacga tagatggagg ccagcttccc tatatgaatg actcacccat cgccgaacca
    11161 tcggccaaaa cgatggtgtt catcgtgccg tcgctcagcc gctcatccgc gcaggatgaa
    11221 gacgtggtgc aactcctgct atgtgccgcg gcgagagtgg aacaactgct atccgcttca
    11281 cctggcttgg ttgtgaaggc gacaatcctg tccgttgatc aagacctcag cgggtgggta
    11341 caggttggag cgctgttctc tgacagaatt tcgaagcacg tcacggttga acccctgaag
    11401 gagccagcaa cgccctgcct cgccattcca gcgcataagt ccgtctatca cgtccatgag
    11461 ttcctgaggg agcgcccctt cgatgaggtg cactgcctgg accgccacgg ctacgtatac
    11521 tactcggcgc acgcgaagag gcttggcctg ttcttcctga cgacgctttt ttgtgttcac
    11581 gttgtggggg ggacgatctt ccgccgcgag tgtcaggatc tgctcctcga cgatcctgat
    11641 atcctggcga ttgatctcct ggagcgtggc acggttgaac gggccgacct ccactacatt
    11701 cacgatatca aagcgtggga gtggtatgac gagaaggttg tcctgcacgc cggcagcacg
    11761 tcttatgatg tcacatggcc tgtggatccg cccacatcgg tggtcgtgga aggcaacgag
    11821 tcttcggaat taccagcact gatctattgt ggaccacttg gcgccgacgg agggttgccg
    11881 ctcttttgcg atgtcgtgga ccgtctcgtg tctcaggggg aacgcctgaa gcatgtgacg
    11941 tttgtcggcg cggctcacgc gatcggcggg gttgatgcgg tcacgtacat ccggctacgg
    12001 agttccggct ggcatctgcc gattacgatc aaacgggatc tttcactcgc tgatgaaatg
    12061 accttcctgg ccggccttca gggagtcgtc gtttgtcata cggctcgacg taaccacctt
    12121 cgttcacgtc tgatcgtggg agggctgcag aatgttctgc atatcagagc agtaaacgaa
    12181 tctcgtgatg gagcgcgcag cggaggtgtc ctccccgggc agataaccca gacggtcact
    12241 cggctcctac gtgcgcccct ccagcagtct cctgtctcgc tccctccgct gacgcaattg
    12301 tggaaagcgg ggcgcaagct tcccgaccat gaaggcgtca ggtcctctct cccattgggt
    12361 gtaccgaatg agcctcagcc cagggtcagc gtctgcatca cgcatttctc gcggccgcag
    12421 aagttacgca tggcgctcca atcgctcaaa cagcagacct atcgcaattt cgacgtgatc
    12481 gtggtggacg acggcagccc ggatcctgac gtccaaaagg aactcgcact aattggagat
    12541 gagatccggc cattgggctg gcgcattctc ttccaagaga accggtatct cggtgcggcg
    12601 agaaacgttg gggcacggca cgcgactgga gagttcttgc tgttcatgga cgacgacaac
    12661 gtcgcaaagc cccatgaact gagcacgttt gtggcaatgg cgcagaggac tggagccgac
    12721 attattacgt cgttttttga cgtatttgaa actgaggcaa cgctggaatc agatgggccg
    12781 ccgcctctcc ggttcactcc atacggagcc gatccggcat tgggtatctt cacgaactgc
    12841 tacggtgatg ccaatgcatt gtattcgcgc cgcgcgttcg acaagttagg aggctttacc
    12901 gaagatttcg gcgtcacgca cgaggactgg gagttattct gccgtgcttc gctcgaaggg
    12961 gtcacactgc tctgcattcc ggaaccgttg ttctggtacc gcatggacca gagcgcgatg
    13021 ttccggggat cccgtacaca actgcataaa agcaccaatc ttcgccggca tatccggccc
    13081 tacctggaaa agctgccacg atatcagagc aatctggtgc aactgctgca gggactttcc
    13141 accacattgc ctctaaccac cgtcggcacg gtctcccgca atgccgcgcc actggctctc
    13201 cgccagcccg atgccacgct tccgtatggc cgggtcgctg tgatcactcg aacgaaagat
    13261 cgcccccttc tgctgcaccg ggccattcgc agcgtgctgt cacaaacatt caaggattgg
    13321 gtgctggtga tcgtcaatga cggcggcgac ccacagcggc ttgagaaggt tgtggacgag
    13381 gtgcgagagg agttagcaga ccgcatcttg gtcgttcatc atccgatgcc gctcggcatg
    13441 caagcggcat ccaattccgg cattgcccat tgcgacagtg acttcgtcat catccacgac
    13501 gacgacgatt catgggaccc cacgtttctc gctcgcaccg tctcttatct ggatgatcat
    13561 gggtggaatg ctcggctagg cggggtgatc acctggtcat gggtgatcat cgaagaagtg
    13621 gccgacgatg gtgcagtgaa agttcacaat cgattcgttt tcaatgacaa gctgcacaat
    13681 gtcgccctca tcgacatgac cgtggaaaac cggtttcctc ctatctcatt cctctttcgt
    13741 cgagctgcga tggatcacgt cggtccattt cgatcccagc atgggccgct gggcgattgg
    13801 gagttccacc ttcggctcct gcagcacttc gatgtcgatg tgatccctga tccactggcg
    13861 aattaccatc atcgcaccaa caccacgatc ggaatctatg gaaactccgt ccatgcacat
    13921 aaagatcaac atcaggcatc gcgaattcat ctgttgaatg aggcgctgag gcgtgatctg
    13981 gattccgggc aggggttgtc cctggcgcaa ctcctgaccc tcggcgaact gcatcacagc
    14041 tcaaaagctg agcaggccag agaatttcag cggctgcacg attacctgtg gaacattgac
    14101 cagcgcgtgc aagctatcgc cacgcaggtg cactcatcca aacatgggct ccgtgcgaaa
    14161 agcagacgca atctggtgtc gaacggggat ttccggcagt ggccagggct gggtcccaac
    14221 cagtttggtc ctcacgagac gtatacgttt cttgatgtca gtcccgggtg tgttctgtgt
    14281 tacgacgggc gccaggtgtc gtatcgtttg gagcaacgca agtggacgac tgacgggcag
    14341 cagttggcgt tcggcaaaac gtttctgcat atcgaaaacg atgggtacac gaagggtgga
    14401 tcatggttcg cgatcgaatt catgattcct tcggcattac tcctttccgg ccacacgatt
    14461 accatctcgg gagtctcgcg cctcaatgcc ctacagggct ggatcggcct cgggggccgg
    14521 tatctgtttg gcgacggccg tcagcttgac tggcccgatc atcgcatcga cgtcgcgtcg
    14581 gactttgccc gatggacctg taccgtgatg tgtccggcca ttcaggagac cgaggtgggg
    14641 cacgggcatc agtcacggat tctcctcaag ctgcctcaca atcaaccctt cgatttcgat
    14701 ctgaccaatc tacaagttga gctgggcagc gaatcgtctg actttgaata cagtagcggg
    14761 aaggtgtccc tacggcacag ggttcatctg gtgctgcgag ggttgaagcg aaggctgcgg
    14821 gcggcgaaac gcgcgatagg atgagttcga actgcgtcgt gaacagacat cgtcggaggt
    14881 acacgtgtcg ggcatctcgt ggaatttgat tcgtcatctg gtggccaggg acttccgcat
    14941 ccggtactcc ggatcagtct tgggtgtcgt ctggtcagtg ctgataccca tggcccaact
    15001 cggggtcctg gtcttcacgt ttggcagcgt ggttccagtc aacatcgacg actatcccgc
    15061 gttcgtgtac agcgccctcc tgccgtggac atggttcagc gcgtgtctcg ggtcaaccgg
    15121 atcgctcttc tttagtcatc gcgacctcgt gcggagaccg aattttcagc ccatcatctt
    15181 aaccgtcgtg aatacgttgt cgaatctcgt cacctttctg ttgagcctcc cactcctgtt
    15241 tcttctgctc gcgtggtatc agcggagcgt ggcggcgagt cttgtcgcac taccggcact
    15301 gctggtcctg caggcggtca tgacggtggg attgagtctc ctggtgtcaa cgtggaatgt
    15361 gttttaccgg gatgtctccc aacttgtgaa tattgtgctc tcgctcttgt ttttcctgac
    15421 gccgattttt tatcgcccgg tagtggaatc caagtacgcc atgatctttc agttcaatcc
    15481 tctggcggtg ctcatttcct cctatcggaa catccttttt gaaggacgtc tgccggagtg
    15541 ggatgcctgg gcgcacatca cggcagtgag tgccgcgctg ctggtctgcg ggtatctcgt
    15601 gtatcgccat gaattgccgg acgtcgtgga cactgtctag gagagaccga tgcacgctgc
    15661 catgacggtc accgacttgt cgaagcgcta ccgtatctca cggcacaagg tgaccacgct
    15721 tcgcgaaacg gtcgatcgct ggatcaaggg ccgtcaggat gtgacgcgga cgatgtgggc
    15781 gttgcgcgat gtctccttca ccatcgaaca gggacgggtc ttcggcatca tcggccataa
    15841 cggagcggga aaaagcaccc ttctccgact gctctgcggc ctgggccggc cgacgtcagg
    15901 ccgtatcgaa gccgggggat atgtgagcgg cattctggaa ctgggcggcg ggtttcatgc
    15961 cgagctgacg ggacgacaaa atctcatgac ggtgggcatc ctcaatgggc tgacgccagc
    16021 cgaggtgaaa gccagggagc aggacatcat cgccttttct gaactggaag actttatcga
    16081 tcaggcggtt cgcacctatt cgaacggcat gtaccttcgt ctcgcattcg ccgcagccat
    16141 cgagtttgat cctgcgattc tgatcattga tgaagtgtta tccgtcggtg atgagcggtt
    16201 tcagcaaaaa tgtctcgatc gcattgcggc cttccgggca tccggcaaga cgctggtcat
    16261 tacgtcgcac gatacggatc agattcagag actgtgcgat gaagtgctcg tcctcgaaga
    16321 gggccgcgcc atcatgcagg gcgaccccaa gagcgcgatc gcttgctacc acgacctgat
    16381 gcgtcaacgt acagaacgcc gggctgctca cattggtggc ggctctacgc gatcactgac
    16441 gactgcaagc gggacgaggc agggcacgca agaagccacc atcgccgtgg tccggttgtg
    16501 ccgcagcgac ggtcaggccg tggagactat acgtagcggc gaagggatcc tggcggaatt
    16561 ggagattgat gcgccaccct caatcgagga cttcgcgctc accgtcgggg tgttcaatga
    16621 ggggcatgtc aaatgttttg aagccaccat cgcatcagta caagggacat tgggcaaagt
    16681 gagccctcac agttccatcc aatgtcatct caaacacctg ccactgttgt ccgggtcgta
    16741 tttcatcaac gtcggcattt acccgacgga ttggtcctat gtctacgatt ttcactggca
    16801 aatgcacccg gtgacggtat ttgacgagcg aacgagcaac tcgcaggcgg gaggaattgt
    16861 cgctgttgaa acacattgga cgatcatctc tcgcggtgat gtatgagacc ggctattcgc
    16921 gcggcagggt tggtctgctg ctgcgtgatc atgcccctgt ggcacgtggt tcaggtgggc
    16981 gccgagacca cgaccgcacc ggcgagcccg cctcgcatgt tgaccaggga aattcgttat
    17041 cacatgcccg aagcagggga agtgtggctg gtgtggggcg tgaacgggtg gcataggctc
    17101 cccgaagcgg cctggccgcc gggaacggtg ttggggaaaa ccggactcat gaccactcgg
    17161 atgatcgaac aaggggacgc gttcgtggcc actgtcgcgg ttccgaacgg tacggcattg
    17221 gactattgct tcctgctcac caaaaagcgc gaggcgtttg atatcacctg gccgctctgc
    17281 gaaggaaatt acagcgaggt cgcgacgcgt tccggtgtga cggaaatcgg atcgcgactg
    17341 tcgttggctg tggtcacaca ggagtttcat taccacatgc ccgatgcgca agaggtgtcg
    17401 atggtatggg gcgtgaatgg atggtctcct gttccggagt cactgcgtcc gctgaacaca
    17461 aagatgtctg acaatctcat ggtgtcaccc atgagaaaga gagagcagga gttcatcgtc
    17521 tcggtgcagg tgcccgtagg ggctacgatc gacttcgggt ttcgaatcac caggtccgcc
    17581 atggggtccg tcaatatttg ggagggcgga ggcgccgaag gatttcatga acctgtgcga
    17641 ggtggttcat cgatccgggt gaaaagcaat gcgatcatcc ttccacgcgg acgtccctct
    17701 cgtgacgaag acaatggatg gcatgtagca tgggcaattg tcggcgcagt gtccgcccct
    17761 ctcctattgg cgggactgct ggtgatgttg agacggcgcc aagcgcggca gcggagcgcg
    17821 aatcccttca accctcgccg ggagaagcca agctcagcac tggacggcct tctgattctt
    17881 gccgctgtgg gcatcggcgc cttcggtgcc gagcaaatgc ttcgaaacgt atatccccac
    17941 gaaggattcg ggattgcggc ggaagacgag tggttccggc aagcccccta tagtgccaac
    18001 caattgttca caatcgatcc cacactcggc atacggccat ggctgcggac gggcaggtat
    18061 acggagtacg gaaccgtgcc caatatgtat cgcctggcca aagattcgcg acatacccgg
    18121 atcctgtttg tcggcaacac cgtgacgtat gagggcacat tgcttcacgc cttaagggaa
    18181 ctccacggag aagaccggtt cgaatattgg aatgccggag tgccgacgtt caacacggtt
    18241 cagcagattg cctactatca tgcgtacaac gcgcgtaccg cgcctgatca tgtcatcttg
    18301 accattcaac cggatgacct cgggaccatc ccgattgtct ttaggaatca cacggcggaa
    18361 cttgaggtgc accgtcctgc gatcccccgg aaagaccttg atacggagct ctacggatcg
    18421 tcttatctgt atcggtatgt gaagggactc ctgatctcac ctgaggagaa gctgcatcgc
    18481 ctgtggcagg agtaccgcga cagcgtaaag agcttcagcg agaggctcgc tcacgaccac
    18541 attcgcttga gtgtcgtagt gcttccggtc ctcgcctctc agcaagagtg gactcaggct
    18601 gagagggacc gacgacaagc gctgttctca gtgctcacgg agttacagat tgaacactac
    18661 gatcttgctc aggcctttga accgggccga gcggagaacg cagtacttcc tccgatgagc
    18721 aaggaaagct gggcgccttc atctgaaatg gtctccgcct ttgctcaata tatgactcac
    18781 gaaggactct ttcgcaccaa ggaacactag catgagtcct catcatacgc atcgccatga
    18841 gaatcgggcc agatccaatc acgcggatga gagcacgcat gatgtccaaa ggggtgattc
    18901 gtcagaatcc atctccggcc tagtctccat cgtgattccc tgctatcagc aggcacactt
    18961 tctcccggac gcgatatcca gcatccttcg ccaaacgtat cccgacattg agatcatcgt
    19021 ggtcgatgac gggtcgacgg atcatacgag agacgtggcg ctgtcacatc ccggaatccg
    19081 gtatatcgct caaaagaacg cgggggtgtc cagcgcgaga aataccggat tccgaatgag
    19141 tcgtggtgag tacattgtct ttctcgatgc cgatgaccga ctcatagcgg acgctatcga
    19201 aatcggcaca cgcagtctcg ccgagcaccc ggcttgtgcg ttgacgttcg ggacattttt
    19261 catgattgac gccgagggca cccgccacaa cccgtcctat cccctgaacg ccgtcactta
    19321 cggctataag gaactacttg aagaaaatgc cattggcaat ccgggggtag cgctctatcg
    19381 ccgatgggcg ctttcggtag tcggcggctt tgatcacagg aacgggcccg ctggagatta
    19441 tgatctgtac ctgcggattg cacggttgtt tccggtcgcg tgccatcggc gtccggtgat
    19501 cgaatatcgc cgtcacgggg gcaacatgag caatgatccg ggggtcatgc tcaaagcctg
    19561 tctctccgcg ctcaataagc agcgcgcatt tatcaagaac caaccggatc ttaccctggc
    19621 gatgcgtcgc ggcagaatcg gctggaagct gtattacggg gagccgttag tcagtaaagt
    19681 ccaagacgaa ttctccaata gaaactggaa agaagggttg cgcggactac tccaactagc
    19741 catctggtat ccggagagat ttctgacagg actgagccgt aaagccacca aactcaaaaa
    19801 caggatgcgc taactggcca tcagtcgtcc gtcaggcgtc ggttatgaag tcctgcccgc
    19861 caaaatccgc ttggcgttcg cgcctagccc tgatggtggt cctctgtggc ctgccgggtt
    19921 gtgtgactga tgcgcccacc ctcaccgaat ccccggatga agggcggaag gtcttgctca
    19981 tggggaatgt ggtcacggtg ctgaccggag acagagcgcg aaagttcgaa ccagcggtac
    20041 gggatatcga attgattgag cagaatgcag gcacgcggta tcgagtccag gtggatggcc
    20101 ccgataaacc gttcgccgtg tttgtgccac caggccgcta tgacattaca cgggtgaaaa
    20161 ttcacgaggg gccattttta tcgatcgcac atctcacggc atcgttttcg gtggaagagg
    20221 ggtcgccgat atttgtggga acctggagat tcggtgtgga ctcgccccgg tatggtcgca
    20281 tggtcgctgt ctccatggtg ctggacgacg aagcccggca acaggcggag caggagctgc
    20341 gcgagaccta tccagacttg gtctcgcccc gaatgctgac cgtcctcccc gcgccgtcag
    20401 agttgcagac acgcttgtat gaagttgctc cttatccgag gatttcgagg tactttcggc
    20461 gccactactg gtgacggtca tgcgagagat gctttccgaa aaagaggaga gggccgcggc
    20521 gcgcgattcc ttcatcggcg aagttgttcc atcgtgcatt ggcgaggtgc tttgtccgga
    20581 cgaaaccgga ggagttttgt gccatggcgg aagcggt
//
LOCUS       DAOQDS010000065        20143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1030_length_20143_cov_22.125348, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000065 DAOQDS010000000
VERSION     DAOQDS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20143
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1030_length_20143_cov_22.125348"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..1134
                     /locus_tag="PK589_14445"
     CDS             391..1134
                     /locus_tag="PK589_14445"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ20858.1"
                     /translation="MKYRGASQLMLGGMAMVLLTAPGCSGMKWFQSGSDESSNSGSSL
                     SASSGASGGYGRGEAGRDGQYPSLGRQDGSSQQNDASDTEGGKLRGFSPLVNGQIAGE
                     ERLSRSPIGSMLTSNEQDAKWAAEIRREEAAALEAGLQDVFYGYDRYNVSDNDGVASL
                     TVNAGWLKENPKALLKISGHCDERGTHDYNLVLGEKRAKAARSFLIDMGVSAKQVAIV
                     SYGKDRPFCADHDEVCYQQNRRGHMLLRK"
     gene            1451..1615
                     /locus_tag="PK589_14450"
     CDS             1451..1615
                     /locus_tag="PK589_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20859.1"
                     /translation="MNQRFGITVKFLISADSPEEAEEIIESACEKMASSIHEGDVSYE
                     GIEDIEEEDE"
     gene            1739..3130
                     /locus_tag="PK589_14455"
     CDS             1739..3130
                     /locus_tag="PK589_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HNJ20860.1"
                     /translation="MTDTVESTGTHSRPPGFLNRIHQSNKQYLPQWLGVPAHIHHVAY
                     RMANPPVERPNSRREFQHLLQALEISEGAIEERFGYGFKTAPNGDRLVVVWEAHTEYY
                     SYQVWHVVGDSNTPLDFGPITFPGYQMPLCPLGLRVNALDLLFLPQELPLDRELPARL
                     PGAMVYGSRILGDDIVAVTSFTPDEFDRERYLICSTSEQALRQQVAKIVDTIVAIENY
                     YHLVMLPMKAFSRAVDQIHDYEQRHLYQRAVLIEQLGGTNPPTMQKWLTVLTQDLLQV
                     SRLAESMRYRLSASVPYDRIVQSNLVALQERPYPPLRQISEYVSWKITGVTDGYQQLL
                     RRIDAMEKDFEATVAVLRTHIELRLQEQNIQLQDQNMKLLASVDSTTRAQAILQRTVE
                     SLSVIVITYYLTGLGSYVLKACYEMGWVKNANVATAIFVPIAFAISFTLMTVGRKIII
                     KRMANPAGDPGGH"
     gene            complement(3222..3584)
                     /locus_tag="PK589_14460"
     CDS             complement(3222..3584)
                     /locus_tag="PK589_14460"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ20861.1"
                     /translation="MDTDLRSSTRVTVTYPVRLSGDSMIGQGTVVNLSAPGCAIETTL
                     PVRPGDYLELHVMAPDQARPLNVGLAKVRWATEQKAGVEFIRVRRDEQSRLQRLIGQV
                     LEESAIGSERDRQELTAA"
     gene            3934..4254
                     /locus_tag="PK589_14465"
     CDS             3934..4254
                     /locus_tag="PK589_14465"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ20862.1"
                     /translation="MERRDTPRYEIKVPLTFSGHAIDGSGLVTSLSKDGCHVTSEESL
                     PMRANLSLHVQFPAPYEPLTVEVAEVRWTNATGFGLVFVHVHDEEHARLQRFIDWLKR
                     TQNN"
     gene            4605..6116
                     /gene="zwf"
                     /locus_tag="PK589_14470"
     CDS             4605..6116
                     /gene="zwf"
                     /locus_tag="PK589_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNJ20863.1"
                     /translation="MDLAQLRTPCVMIIFGIDGDLAKRKLLPALYNLMAGHFLPEQFA
                     IVGLDRSETTTEEFRAKLDRLMPEYLPENADRAVWQAVSQRVHYMAGDFQEGATYRRL
                     NDLLQGVDASAGTQGNYIFYMATIPGLFGRIVTHLGEYGLTVERDGTWRRVIIEKPFG
                     HDLDSAKALNHELQRVLQERQIYRIDHYLGKETVQNILVFRFANGIFEPLWNRRYIDH
                     VQITVAESLGVEHRGRYYEQAGAMRDMVPNHLLQLLCFLAMEPPNSFAADAVRDEKAK
                     VLRGVVPLSSLDVLRFVAFGQYGEGAVDGKPVVGYRAEPHVGAESTTETFVAMKLFID
                     NWRWAGVPFYLRTGKRMTRRLTEIVVQFKQAPFMLFRKTQVDRLAPNVLVIRIQPDEG
                     ICLRFDAKVPGPTVRIGEVNMDFQYADYFGNAPQTGYETLLHDAMAGDATLFQRADNI
                     ELGWSVVQMILDVWKSLPGSAAFPNYQSGTWGPPEAEALLRREGREWWNGGQA"
     gene            6149..6694
                     /gene="msrA"
                     /locus_tag="PK589_14475"
     CDS             6149..6694
                     /gene="msrA"
                     /locus_tag="PK589_14475"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNJ20864.1"
                     /translation="MAQAGMEVATLAGGCFWCLEAVYDQVKGVQSVESGYIGGQIDEP
                     TYEQVCGGRTGHAEAVRVTFDPAVVSFRDLLEIFFVIHDPTTRNRQGNDVGTQYRSAI
                     FYHTPEQQQAAQEMIAAVTQEHLYPNPVVTEVVPATRWFEAESYHQEYFARNPLEGYC
                     TYVVGPKVAKFRQRFLALRKP"
     gene            complement(6709..7146)
                     /locus_tag="PK589_14480"
     CDS             complement(6709..7146)
                     /locus_tag="PK589_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20865.1"
                     /translation="MNPVQQSPRQGAEPPLWSIAAAAAVILLTPMLLFSLAPDGPIRD
                     GDTVFSTGAHKVPLFKPEQHRQAGYDATCLLDPKDPMIVLHTPAEGSDEEDIIAQVQG
                     KNTIEWPFCPPQAELIIKPYHVTQQPSMIKDLRDGFLRLFKSS"
     gene            complement(7851..8726)
                     /gene="ttcA"
                     /locus_tag="PK589_14485"
     CDS             complement(7851..8726)
                     /gene="ttcA"
                     /locus_tag="PK589_14485"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNJ20866.1"
                     /translation="MQPTSTKPVPAPARPAAQSEEIERVETRLCRLAGQAIADYRMIE
                     DGDKVMVCLSGGKDSYGLLEILLSLRSRAPIHFDIIAVNLDQKQPGFPAHVLPEYLTR
                     RGVPFHIETRDTYSVVKRLIPEGQTTCSLCSRLRRGHLYRLSTELGATKIALGHHRDD
                     ILETLLLNLFFTGKMKAMPPKLRSKSGRHVVIRPLAYVKEADLARYAALLQFPIIPCD
                     LCGSQEDLKRKQVKQMLQDWNQRFPGSNDSMFAALGNIAPSLLLDRSLFNFSDLKADA
                     ASPDASVSDAEEDLL"
     gene            9005..10066
                     /locus_tag="PK589_14490"
     CDS             9005..10066
                     /locus_tag="PK589_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HNJ20867.1"
                     /translation="MGRTQIHRRQRSEPGGRVPVWAEWTDEQLLDLRMCDLDLRLDGT
                     FYQEPIEQLYRELDARRLVFRPHFWLSDEWFTPDGVPGIAVPFYLAHPRLAKLEASQM
                     LEVEGGTHDWCMRILRHEAGHAIENAFLLRRRRRRQKLFGRSSQPYPEYYTPRPYSRS
                     FVRHLDVWYAQSHPDEDFAETFAVWLDAGSLWKERYRGWPVLKKLDFMERLMGELAGV
                     APMVTGRQQLDPLPRIYKTLRDHYEEKRKHYGIGRASSYDTDLNKLFSAGVAYAKNPS
                     AAEFLRRTRKEIRRRVAEWTGEYQYTIDQVLEGMIERCHASKLHLTQQTEQAKLDFAI
                     LLTVQTMNYLHSGRHKVAL"
     gene            10063..11085
                     /locus_tag="PK589_14495"
     CDS             10063..11085
                     /locus_tag="PK589_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNJ20868.1"
                     /translation="MRRMRVLVLMHKDLVPPEQLNGQDLDTAAWKTEYDVVSTLRKLG
                     HDVQALGVKSDLEVIRAAVEDWKPHIAFNLLEEFDGVAVYDQNVVSYLELMRIPYTGC
                     NPRGLMLARDKALAKQVMSYHRIPYPEFMVVPLHRMVRRPKYLPFPLIVKSITEEASL
                     GISQASIVDDDAKLRERVAFIHDNVGTGALVERYIEGRELYVGVMGNAHLQVFPVWEL
                     VMDKMPDESRRIATQRVKWSRKYQDKYGIRSCEARNLPDGVIDHIQHLAKRVYRALGL
                     SGYARIDMRMDQAGTVYVIEANPNPQIASGEDFADSAEKANLAYKDLLQELLHVGLRW
                     RPAQAA"
     gene            complement(12164..12715)
                     /locus_tag="PK589_14500"
     CDS             complement(12164..12715)
                     /locus_tag="PK589_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20869.1"
                     /translation="MTYARRIRLFLAGLAFIGFLSPCAAQSADPQEPHWGFATDLGLW
                     TGTTNNTTFALGFGLDYYMDPNFSFGGMALFTPVGDLTQIGVAGVAKYHLRLNSGFNL
                     VPFAGLGILHADLNRGSGPGKIDRNDTSHFIPLGMSLEYQVGPKIAFSTTLMVNLHHI
                     TLSPPVPNDNTSVALLFGIRWGP"
     gene            12856..13350
                     /locus_tag="PK589_14505"
     CDS             12856..13350
                     /locus_tag="PK589_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20870.1"
                     /translation="MKKESMTPAEAADALYKLMPRRITVEMLSDYGIQGEDAHEDAMT
                     RELLSFTLYWVLAAINAHIPRKYRDLVFHRVMDLIRADWETEYKLGALKWEEYLDEVE
                     ARRARYAPVGDYEGGAIAASEEISDLLETEGLIQPEDRPKLLVLLPDLVPLDKYQELL
                     SQCV"
     gene            complement(13397..14002)
                     /locus_tag="PK589_14510"
     CDS             complement(13397..14002)
                     /locus_tag="PK589_14510"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNJ20871.1"
                     /translation="MSKLVLIRHGESQWNLENRFTGWVDVPLSPKGIEEAKAAGKKLA
                     GFTFDRAFSSVLARANETLRFILEAIGQTTIPVEKDKALNERMYGELQGLNKAETAKK
                     FGDEQVKIWRRSYDVRPPGGESLKDTAERVLPYYESRIKPYVLKGETILIAAHGNSLR
                     ALVMQLEQLTREQVLELNIPTGAPLLYELDTNGKVQSHRYL"
     gene            14193..15026
                     /locus_tag="PK589_14515"
     CDS             14193..15026
                     /locus_tag="PK589_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20872.1"
                     /translation="MPDVSPSDWSSLQQWWQEMGRRLQVEDRVEAFFRRAMGASQALA
                     GAVQVGGEVEYILFVLVRYWIPVVLPPPGRERASKGDPDYWLESEQILKAAAVRLREL
                     KPLIELLTASNPLSAGVAEPQAARPPMVEVELANMLQGIAESAGSYGGPDYTSVIKTF
                     DPVPLRQTQPFKHNKKNSAELWVVFLLREHFRSLGLGKDRYWPLVAGLCAAAGIANPN
                     GQPYGPDELKSWWQKNWPRTYTQLEQTQAAPDLSGAAYQQDFDWFVSWIEWQRQQISE
                     V"
     gene            complement(15050..15691)
                     /locus_tag="PK589_14520"
     CDS             complement(15050..15691)
                     /locus_tag="PK589_14520"
                     /inference="COORDINATES: protein motif:HMM:NF022400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNJ20873.1"
                     /translation="MKTCSQCRQENRDDARFCHQCGAPFALEARAAASETEQDAPQTD
                     TELLKAFIGPNADRYLETFKKFSGPGGPQFALSWHWPAFVFEPFLWFLYRKMYVYALI
                     YAIGPAMAFYITQDLSADIVWRVIAGGSANYIYFWHVKEQLAKIKSERATGGETREQL
                     LGELGGVQPYVVWVGVGLLVLKIGLVVAMFKEGPPDGPKGPPAKPHPASLTHV"
     gene            15860..17065
                     /locus_tag="PK589_14525"
     CDS             15860..17065
                     /locus_tag="PK589_14525"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HNJ20874.1"
                     /translation="MPTGELRLNAEQYLMNTYQRQPISIVRGRGSKVYDLEGREYIDF
                     VAGIAVNVLGHGHPDLVLAIQKQAQHLLHTSNLYYTEPQVRLAQTLVEHSFAQKVFFC
                     NSGAEANEAAIKLARKYSYDKYGADRHEIITMTSSFHGRTMATLTATGQDKVQKGFAP
                     LMPGFSYVPFNDLSALERAITPKTAAIMLEPIQAEGGVHVAERGYLQGLRELCRERDV
                     LLIFDEVQTGIGRTGTLFCYEQFGMQPDIMTLAKGLGGGVPIGACLATDSVARAFGPG
                     THASTFGGNPLACASALAVLRVLLDGKILDQGRRMGEYLAKGLATLKERHRCIKEVRG
                     MGLLQGMELDVDGKTVVADCLTRGLLINCVGERVLRFVPALIITEREIDRLLASLSQI
                     LSQRTTSTH"
     gene            17082..18062
                     /gene="argF"
                     /locus_tag="PK589_14530"
     CDS             17082..18062
                     /gene="argF"
                     /locus_tag="PK589_14530"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNJ20875.1"
                     /translation="MLRRLRRSTPRTGLGKDFLDLLSIPTDELTGLLRLAAQLKVKQR
                     RGVSHPLLQGRMLGLLFQKPSTRTRVSFEAGMNQLGGQAMVLPMGDIQLSRGESIADT
                     AHVLSRYLDAIVLRTFDHAIAEEWAREASIPVINGLTDLNHPCQALSDLLTIQEKKRR
                     LKGLKLAYVGDGNNVTNSLIEAAAKMGMTIAVGCPPGYQPDRRIVELSLAEAERTGAV
                     IEIGSDPSVAVKDADVVYTDVWISMGQEREQAKRLKTLAPYQLNARLLKCAKPDALVM
                     HCLPAHRGEEISAEVLDGPQSVVFDQAENRLHMQKAILVRLLGKKTARKT"
     gene            18077..19294
                     /locus_tag="PK589_14535"
     CDS             18077..19294
                     /locus_tag="PK589_14535"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNJ20876.1"
                     /translation="MSQSSYKKVVLAYSGGLDTSVILKWLEEVYGCEVVAFCADLGQG
                     EDLKAIKKKAQSLGVKKVYVEDLRETFVKDHVFPMLRGNAIYEGSYLLGTSIARPLIA
                     KRQIEIAAKEGAAAVCHGATGKGNDQVRFELTYMALHPQIKIIAPWREWTMRSRRELI
                     EYAEKHGIPVTATKAKPYSMDMNLFHTSYEGGILEDPWEAPPEEIFVMSVSPERAPAK
                     AREVEIEYVAGNPVAVDGKKMSPAALLGHLNKLGGEHGVGRVDLVENRYVGMKSRGVY
                     ETPGGTILHAAHRGLESLTMDREVLHLRDSLIPRYAELIYYGYWYAPEREMLQVALDE
                     AQKDVTGTVRVKLYKGTCTVVGRKSPRSLYRLDMATFEEDDVYRQKDAEGFIRLNALR
                     LAIRAQRKKRPGR"
     gene            19294..>20143
                     /gene="argH"
                     /locus_tag="PK589_14540"
     CDS             19294..>20143
                     /gene="argH"
                     /locus_tag="PK589_14540"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNJ20877.1"
                     /translation="MGKSTPVGARGRKSARAPQRSGRALPKGKAWGGRFAEQTDRLVE
                     QFSSSIAYDRRLYPYDIQGSIAHCKTLERAGVLTGRESGQLVRGLQFVKVELDGGRFP
                     FSPQDEDIHMAIERRLTELIGPVGGKLHTGRSRNDQVALDLRLFLRDVLSTFMKQVQE
                     FRRVLVGQARAHLDVVMPGYTHLQRAQPVLLAHHFLAYVEMLDRDRTRLHDCRQRLNV
                     MPLGSGALAGSNYPVDRRYTAALLEFPSVTQNSLDAVSDRDAVVEVLSTLSLIMMHLS
                     RLSEELI"
BASE COUNT         4085 a         5484 c         6221 g         4353 t
ORIGIN      
        1 gagcatcgga cttcggatcc gagggttggg ggttcaagtc cctccgggcg caccaagagt
       61 gtgtgccgct ctccgcacac atgtgtacgc ctacgccata gcgtctccac ctcagtaact
      121 ccctcgaccc tgactctctc ccgtacaagg cttctagctg tctaaccctc cgagcggcta
      181 cgtgcctcgc tgcgttttta ctcggaatcc ttgggaaatg aagagaatat gaagctggag
      241 ggggctgaat actgaattgc caagacgata taactatacg gaatgccgag tatttctgga
      301 ggctccgcgc tgaaaccagc gtaaatcgag gccggtgcta tatttgttac gaacagcaca
      361 ggttacaccc cctcgcaagg agcaaggttc atgaagtacc gtggcgcgtc gcaactgatg
      421 ttgggcggaa tggcaatggt cctgttaacg gcaccgggct gtagcggcat gaagtggttt
      481 cagtccggct cggatgagtc ctccaattcg ggttcgtctt tgtcggcgtc atccggtgcg
      541 tcgggcgggt atggacgcgg ggaggcgggg cgagacggac agtatccctc acttggccgg
      601 caggacggct catcccagca gaacgacgca tcagatacgg aaggcggaaa gttgcgcggc
      661 ttttctcctc tcgtgaacgg ccagatcgcc ggtgaagaac gcctgagccg gagcccgatc
      721 ggcagtatgt tgacgtcaaa tgagcaggac gcgaagtggg ccgccgaaat tcggcgtgaa
      781 gaagcggccg cgttggaagc cggcctccag gacgtgttct acgggtatga tcgttataac
      841 gtgtcggaca atgatggggt ggcgtcgctc accgtcaatg ccggttggtt gaaggagaat
      901 cctaaggccc tcctcaagat ctccgggcac tgcgacgaac gtgggaccca tgattacaat
      961 cttgtcctcg gtgaaaaacg tgccaaagcg gcgagaagct ttttgatcga catgggcgtg
     1021 agtgcgaagc aggtggcgat cgtgtcctac ggcaaagatc gccccttctg tgccgaccat
     1081 gacgaagtgt gttatcaaca gaatcggcgc ggccatatgt tgctgcgcaa ataggcgaga
     1141 gtctccggcg cccgcccgcg gggtgccggg gccaggtttc ggcgagttgc tccttccagc
     1201 ggcctttgct caatagcagg ttccccatcg caccctctcc gtccttcaga gaacgcccgt
     1261 ccatcctgac ggtccgttaa cgcctctttc gaagccgcag ttctgctttt ctgaccgttg
     1321 tcagggccga tgaatcacgt gcgggtttcc cctcttgcga cagaagggtg acaatacgcg
     1381 ggagcttatg gtattgagcc acagcaggcg acagactttc tgccgcctat ttcgccgaag
     1441 gagtacgatt gtgaaccagc gctttgggat taccgtgaag tttttgattt cagccgactc
     1501 tcccgaggag gccgaagaga ttattgaatc agcctgtgaa aagatggcgt cgtccatcca
     1561 cgagggcgat gtcagttacg aaggcattga agatatcgaa gaggaagacg agtaatgacg
     1621 aagtccgttc cattcgcctc tgccgcctgt ctccgatgcc ggcctcgtca cgaggggcga
     1681 tcggattgga gaggctcggc agcgccctgg gccgggttca gtgggagaca gaggtgatat
     1741 gacggatact gtcgagtcga ccggaacgca ttcccgcccc ccggggtttt tgaaccggat
     1801 ccatcaatcg aataagcagt acctgccgca atggctcggc gtgccggcgc acatccatca
     1861 tgtcgcctac cggatggcga atccgccggt agaacggccc aatagccggc gggaatttca
     1921 gcatctcctc caggccctcg aaatttcgga aggagccatt gaggagcggt ttggctacgg
     1981 gttcaagacg gcgcccaacg gcgatcgtct cgtcgtcgtg tgggaggcgc acacggagta
     2041 ctacagttac caggtctggc atgtggtggg ggactcgaac acgccgctcg atttcgggcc
     2101 gatcacgttt cccggctatc agatgccgtt gtgtcccctc gggctgcgcg tgaatgcgct
     2161 cgatcttctg ttcctgccgc aggagttgcc gcttgatcgc gaactgccgg cgcggcttcc
     2221 cggcgccatg gtgtacggga gtcggattct gggagacgac atcgtcgcgg tgacgagctt
     2281 tacgcccgat gagttcgacc gggaacggta cttgatttgc tctacctcgg agcaggcgct
     2341 tcggcaacag gtggcgaaga ttgtcgatac gatcgtcgcc atcgaaaatt attaccactt
     2401 agtcatgttg ccgatgaagg cattttcgcg ggccgtcgat caaatccatg actatgagca
     2461 acgccacctc tatcaacggg cggtgttgat cgagcaattg ggcgggacga atcccccgac
     2521 gatgcagaaa tggctgacgg tgctcaccca agacctgttg caggtgagcc ggctggcaga
     2581 gtcgatgcga taccgcctct ccgcctcggt gccgtacgac cgcatcgtcc agagcaacct
     2641 ggtcgcattg caggagcggc cttatccccc gctgcgccaa atttcagagt acgtcagttg
     2701 gaagatcacc ggggtgaccg acggctacca gcagctgctc cgtcgcatcg atgccatgga
     2761 gaaggatttt gaagcgaccg tggcggtgct gcgcacgcat atcgagttgc ggttgcagga
     2821 gcagaatatc caactgcagg atcagaatat gaagctgttg gccagcgtgg attctacgac
     2881 gcgggctcag gcgatcctgc agcgcacagt ggaaagtctg tccgtgatcg tcatcacgta
     2941 ctatctcacg gggctgggca gctatgtgct gaaggcctgt tacgagatgg gatgggtgaa
     3001 gaatgccaac gtggccacgg cgattttcgt tccgatcgcc ttcgcgattt ccttcaccct
     3061 catgaccgta gggcggaaaa tcatcatcaa gcggatggcg aatcccgccg gtgatcccgg
     3121 cgggcattag attcggactg ctaccttctc aactttcccg cacatcatcg tcactttttc
     3181 gagagaaggt ctggcggcca tgtctggcct tgccgtgccg atcaggcagc cgtcaattcc
     3241 tgtctgtccc gctcagatcc gatggccgac tcttcgagga cctggccgat caatcgctga
     3301 agtcggcttt gttcgtcccg gcgcacgcgg ataaattcga cgcctgcctt ttgctcagtg
     3361 gcccagcgaa ccttggccag ccccacattc agcggtcggg cctggtccgg cgccatgacg
     3421 tgtaattcga gataatcgcc gggtcggacg ggaagtgtcg tttcgatggc gcaaccgggg
     3481 gcggagagat tgacgaccgt gccctgtccg atcatcgaat cgccggaaag cctgacggga
     3541 taggtaacgg tgacgcgagt ggatgaacga agatccgtgt ccatgtgaag gcctccttgc
     3601 cttgggccct ctactcctga gggcgtacag accttttcgg tagggtattt cccgaacttt
     3661 agccagaaag actgagcaat cgatcaattt cccggccaag gccgagcgcc cggccggatt
     3721 tggtatgcgg ctgatggcag gagggttccc cgcggactcc ttgccagggc ctttttgctg
     3781 gaacgcgtag accgtagggc tggcaagcga ggcagagaag gcagatccgg tcccttcccg
     3841 tgaaaggcgg ttgatttcat gggcggaaga gcgtatccat cagcggacgt ttctcgaatc
     3901 gaccaccaca gcagcatcgg atgaaaggtt cccgtggagc gccgcgatac accacgctat
     3961 gaaataaagg tgccgctcac attctccggc cacgccatcg acggcagcgg gcttgtgacc
     4021 agtctttcca aggatggctg ccatgtcacg agcgaagagt cgcttcccat gcgcgccaac
     4081 ttgtcgctac atgtccaatt ccccgcgccg tatgaaccgc tgacggtcga agtcgcggag
     4141 gtgcgatgga cgaatgccac gggcttcggc ctggtgtttg tgcatgtgca tgacgaagag
     4201 catgcgcgct tgcagcggtt catcgactgg ttgaagcgca cccagaacaa ttaacgctgc
     4261 ggtcgctcgc cgttggccaa ttgcgcctgt ctttcgcgaa cacgctcact cgtcccatcg
     4321 tgacattctc ttccgaaacg atctcctcgt gacgattgtc tgagttgaag gcccgaactc
     4381 ttctctgaca cgcgtgccgg ccattcagtc agccgcgtca tgcgcgttca ttatcgcgaa
     4441 gacgtcgtgc tgcatgagcg gatccgcacg atcaggtgtc agattccacg acgtcctcac
     4501 caaaaaaatc tcaacgagac tgtgcggtgg ttgcggttag gggagggatc aggcaggata
     4561 ccgtatgtcc ccacaacaat ctcggaacta accaggatcc tttcatggac cttgcacagc
     4621 tacgcacgcc ctgcgtcatg atcatctttg gcatcgatgg cgaccttgcc aagcggaagt
     4681 tgctgcctgc tctgtacaac ctgatggcag ggcatttcct tccggaacaa ttcgccatcg
     4741 tgggtttgga tcgctcggaa acgacgacgg aagaatttcg cgcgaagctg gatcgactca
     4801 tgcccgagta tctgccggaa aacgccgatc gcgccgtatg gcaggccgtc tcacagcgtg
     4861 tacattacat ggccggggat ttccaggaag gggcgactta ccgccggctg aacgacctgc
     4921 ttcaaggggt cgatgcttcc gccgggacgc agggcaatta tattttttac atggcgacta
     4981 tccccggcct gttcggtcgg attgtgacgc acctggggga atacggcctg accgtggaac
     5041 gagacggcac ctggcgccgc gtcatcatcg agaaaccgtt cgggcatgat ctggattcgg
     5101 caaaagccct caatcatgaa ttgcagcgag tgctgcagga acggcagatt tatcggatcg
     5161 atcactacct cggtaaagaa accgtgcaga acatcctggt gttccggttt gcgaatggga
     5221 tttttgagcc gttatggaac cggcggtata tcgatcacgt gcaaatcacg gtggcggaga
     5281 gcctcggggt cgaacatcgc ggccggtatt atgagcaggc gggtgctatg cgggatatgg
     5341 tgccgaatca tctccttcag ttgctctgct tcctggcgat ggaaccgccg aactcttttg
     5401 ccgcggatgc cgtgcgcgac gagaaagcca aagttctgcg cggagtcgtg ccgctcagtt
     5461 cgcttgacgt gttgcgtttt gtagcatttg gccagtatgg cgaaggtgcg gtcgatggga
     5521 agccggtagt cgggtatcgc gcagagccgc atgtgggcgc cgaatcgacg acggagacct
     5581 ttgtggcgat gaaactgttc atcgacaact ggcgctgggc cggagtgccg ttttacctgc
     5641 ggacgggaaa acgaatgacc cggcgattga ccgaaattgt ggtgcaattc aagcaagcgc
     5701 ccttcatgtt gtttcgcaag acccaagttg atcgtttggc gccgaatgtg ctcgtcatcc
     5761 gcattcagcc cgacgagggc atttgcctgc gtttcgatgc caaggtccct ggtcccacgg
     5821 tgcggatcgg ggaggtgaat atggattttc agtacgccga ctatttcggg aatgcgcccc
     5881 aaaccggcta cgaaacgctg ctccacgacg ctatggccgg cgacgcgacg ctgtttcaac
     5941 gggcggacaa tattgaatta ggctggtccg tcgtgcagat gattctggat gtgtggaaat
     6001 ctctgcccgg atcagcggcc ttcccgaatt atcagagtgg aacctggggc ccgcctgaag
     6061 ccgaggcgct gttgcggcgc gaaggccgtg agtggtggaa tggcgggcaa gcgtagatgg
     6121 tgggtcgaaa tcgaaagcga ggtcgttgat ggcgcaggcg gggatggagg ttgcgaccct
     6181 ggcggggggc tgcttctggt gtctggaagc agtctatgat caggtcaagg gtgtgcaatc
     6241 ggtagaatcc ggatatatcg gcgggcagat agatgagccg acgtatgaac aggtgtgcgg
     6301 cgggcggacg gggcatgccg aagcggtacg tgtgacattt gatcccgccg tggtcagctt
     6361 tcgagatctg ctcgagatat ttttcgtgat tcacgatccg acgacgcgaa atcggcaagg
     6421 aaacgatgtc ggcacccagt atcgatcggc gatcttctac catacgcctg aacagcagca
     6481 ggccgcgcag gagatgatcg cggccgtgac tcaagagcat ctgtatccca acccggtggt
     6541 caccgaggtg gtgccggcga ctcggtggtt tgaagccgag tcctaccacc aggagtactt
     6601 tgcccgcaat cctctcgaag gctattgcac gtatgtagtt gggccgaaag tcgcgaaatt
     6661 ccgccagcgg tttctggcgt tgcggaagcc ctgagctccg cggtctggct aggaagactt
     6721 gaagagcctc aggaagccgt ctcgcagatc tttgatcatg ctgggctgct gggtgacgtg
     6781 gtacggtttg ataatgagct cagcctgcgg aggacaaaac ggccattcaa tcgtgttttt
     6841 gccttggacc tgcgcgatga tgtcttcttc atccgacccc tccgccgggg tatgaaggac
     6901 gatcatcgga tctttcggat caagcagaca ggttgcgtca tatcccgcct gacggtgttg
     6961 ctccggttta aacagaggta ccttatgagc cccggtggaa aagaccgtgt cgccatcgcg
     7021 aatcggcccg tcgggggcga gcgaaaacag cagcatcggc gtcaacagta tcacggctgc
     7081 tgcggccgca atggaccaca gcgggggctc ggctccctgc cgaggggact gttgaaccgg
     7141 gttcatactc gcggatggcc cccatgcgat aggacttgct tccacgtgga aactgtttca
     7201 cactcacgcc cccgcaccgg ggtcatctta cccgatgcga aggagaaagg cgcgtgacga
     7261 cagtctccat ggtgtattgt ccaatgtgct ctggtagcgc caagcgagtg acccgtcatg
     7321 gttggcgcga tttcgtgatg cgtgtgatcg gcttctatcc gtttcgatgt acgctgtgtc
     7381 tctaccgctt ccgcttgcgc cgccgcacct gatgcccgcg aggagatgcg tcttgttcga
     7441 aatgaagaca gggtgtgcgg acttgccata ggtgtccggc tacccacgat gggcctgtcg
     7501 tggaaacttg acttgcatcg cctgagggtc tttcagtagg attctccctt ggttgtccat
     7561 ctggcctttg cgcaagtgag tgggggatat gtcgtactgc ggtaagtgct ccgggtatac
     7621 aaaacgttcg cgccgtcagg ggctgctgga ttttttcttt ttcctgatcg gctactatcc
     7681 ctaccgttgt ttgcgatgtg gtcggcgaac ccatcttcgc cagcgcgtct gatcgttcct
     7741 tctgcccctg ttttttcctt cacatgtcca gcgaaggttg cgtggcctgc gacagtgata
     7801 tacctagcgc tggttccatg ctggaactgc ggcgcgtcac agatctacag ctatagcaga
     7861 tcctcctcgg catcgcttac agaagcgtca ggggaggcgg cgtccgcttt caggtcagaa
     7921 aaattgaaaa gtgaacgatc caggagcagg gagggcgcga tgttccccag ggcggcgaac
     7981 atgctgtcgt tggaaccggg aaaacgctga ttccagtcct gcagcatctg cttgacctgt
     8041 ttgcgtttca aatcctcctg cgagccgcag aggtcgcagg ggatgatcgg aaactgaagc
     8101 agggccgcat agcgagccag gtccgcctct ttcacatagg ccaacggtcg aatcaccacg
     8161 tgacggccgc tcttggagcg caacttcggc ggcatcgcct tcatcttgcc ggtgaagaaa
     8221 agattcaaca gcagcgtttc gagaatatcg tcacgatgat ggcctaaggc gattttggtc
     8281 gcgcccaatt ccgtggacag ccgatagaga tggccgcgcc gcaggcgtga acaaagcgag
     8341 caggtggtct gcccttccgg aatcaatcgt ttgacgacgg aataggtgtc gcgcgtttca
     8401 atgtgaaaag gaacgccgcg gcgagtgaga tactccggca gcacatgggc aggaaatccc
     8461 ggctgcttct gatccaggtt gacggcaatg atgtcgaagt gaatcggggc ccggctgcgt
     8521 agcgacagca gaatttccaa gagtccgtaa ctgtctttcc cccccgacag acacaccatc
     8581 accttatcgc catcctcaat catgcgatag tccgcaatgg cttggcccgc gagacggcag
     8641 aggcgggttt ccacccgttc gatctcttcg gattgtgcgg ctgggcgcgc aggggctgga
     8701 acgggttttg tgctggtcgg ctgcatggcc aggcattcta gctcaagaag gggcgcgaat
     8761 gggataggcc gaatgctcat cgagggtggc gcgggaattc agcgattcct acggacgatt
     8821 cccaatttta gacgccggcg tgaatcacgt cccgtcatta tcaccaggcg gcgttcaccg
     8881 ctccgagggt tttccctgag cctcgacgtg actcgcccag tgatcgggca gcacaattct
     8941 tgcgagctgc tatagttgaa acagaacaaa agggcgccgg acggggcttt ttggtggttg
     9001 cacgatgggg cggacacaga ttcatcgacg acagaggtct gaaccgggcg gtcgggtgcc
     9061 ggtctgggca gagtggacgg acgaacagct gctcgatctt cgcatgtgcg atctggatct
     9121 gcgcctcgac ggcaccttct atcaggagcc gatcgaacaa ctgtatcgcg aattggacgc
     9181 gcgccgattg gtcttcaggc cgcacttctg gctctcggat gaatggttta cgccagacgg
     9241 ggtaccgggt attgccgtcc cgttttacct tgcgcacccg cggttggcca agctggaagc
     9301 cagccagatg ttggaagtgg aagggggaac tcacgactgg tgcatgcgca tcctgcgcca
     9361 tgaggccggg catgcgattg aaaacgcgtt cttgctgcgc cgtcgccgcc gtcggcagaa
     9421 actcttcggc cgctcctctc aaccctatcc cgaatactac accccgagac cctatagccg
     9481 gagtttcgtc cgccacctgg atgtctggta tgcccagagc catccggatg aagactttgc
     9541 cgaaacgttt gccgtgtggc tggatgcggg gtcgctctgg aaggagcgct accgtggttg
     9601 gccggtcctg aagaaactgg atttcatgga acggctcatg ggtgaactcg ccggcgtggc
     9661 gcccatggtc acgggtcgac aacaactcga tccgctgccg cgcatctaca agacgctgcg
     9721 ggaccattat gaggaaaagc gaaagcacta cggaatcggc cgggcctcct cctacgacac
     9781 ggacctgaac aaattgtttt cggccggagt tgcctatgcc aaaaatccca gcgccgccga
     9841 gttccttcgc cgaacgagga aggagatccg ccgccgggtg gctgagtgga cgggagagta
     9901 ccaatacacg atcgatcaag tcttagaagg catgatcgaa cggtgtcatg cgtcgaagtt
     9961 gcatctgacg caacagacgg agcaggccaa actcgacttt gcgatcctgc tgaccgtgca
    10021 gaccatgaat tacctgcaca gcgggagaca taaggtggcg ttatgagacg aatgcgcgta
    10081 ctcgtgctca tgcataagga tctggttccg ccggagcaac tcaacggtca ggatctggac
    10141 actgccgcgt ggaaaaccga gtatgacgtg gtctcgactc tgcgcaaact tggccatgac
    10201 gtgcaggcgc tcggtgtgaa aagtgacctg gaagtcattc gcgcggcggt agaggattgg
    10261 aaaccgcaca ttgccttcaa tctactggaa gagttcgacg gcgtggcggt gtacgaccag
    10321 aatgtcgtct cctatctcga attgatgcga attccttaca ccggatgcaa cccgcgcgga
    10381 ctcatgctgg cgagggataa ggcgttagcc aaacaagtga tgtcctacca tcggattccc
    10441 tatccggagt ttatggtcgt gccgcttcac cgcatggtgc gtcgtcccaa atatctgccg
    10501 ttccccttga ttgtgaagtc gatcaccgaa gaagcgtcgc tgggcatttc gcaagcctcc
    10561 attgtcgacg atgacgcgaa gttgcgcgag cgggtggcct tcattcacga caatgtcgga
    10621 accggagccc tcgtcgaacg gtatatcgaa gggcgggagt tgtatgtcgg cgtgatgggt
    10681 aacgcgcact tgcaagtgtt cccggtctgg gaactggtga tggataagat gccggatgag
    10741 tcacggcgca tcgccactca acgcgtgaaa tggagccgca agtaccagga caaatacggg
    10801 atccgttcat gcgaagcgag gaatttgccg gacggagtga tcgatcatat tcagcatctg
    10861 gccaaacgcg tctaccgtgc gttaggactg agcggctacg cgcgcatcga catgcggatg
    10921 gaccaagcgg ggacggtcta tgtgattgaa gccaatccga atcctcagat cgccagtggt
    10981 gaagattttg ccgactctgc cgaaaaagcg aacctcgcct acaaagacct gctacaggaa
    11041 ttgctgcatg tggggctcag gtggcgtcct gcccaagccg cgtagcggat gttgaaacgg
    11101 gctggctgct gcgttctcgt tttgctcaaa acttcgacgt actcgaatgg tacgcctcag
    11161 cttttcgctc actgcggcct tgcagcaaga cccgtttgaa catccgccag agcagccgct
    11221 gaaacgtctc cccagcttcg ttctcgtctc gctcagagac tcaacgtacc gcaagggtac
    11281 acctcgcctc ctcgctctgc tgcggcctcg cttggaaccc gtttgagcgg ctgctccaac
    11341 cttttcgaag ccgtgagtcg tgacggtggc tttgctgaag agttggcgtt ttacaagagg
    11401 agcgtgcttc cgctaccgtg agacttagtc gtggcctgtt agaatcggtg cccaggtatt
    11461 tatgcaacgc ggtctattat gcgaggcaac ggttacgtac agtgaagtcc cacccctcgg
    11521 ctaatctggc gcagatgcag cgtatggtcg atcgtcccat cactacaatg atgcagatac
    11581 ttcgtcccct gtcggtggtg tgtttgctca tgctcggtgg ctgcgtttcc gtatggcatg
    11641 tggatgtttc gaatcagggg aatctagaca tctttcatct gcgtcattcg cacggggtat
    11701 tctttccctt tatgatcggt gaggacgacc tttatctcta cgtgccggtg tattcacagc
    11761 aggaagggaa taggtttctc atcccttctt tgtcgggaaa agcttcactt tgtcgatatg
    11821 cgcatcctat aaagcggtgc acagatcagg ttgaaggaga ggtcggggtt ttgcgagttt
    11881 cggatcattg gatcgagttg tctatttctc tttctgcaaa tcaggaacat tcaatgacgc
    11941 catggagcat gcatcgcgga tgggcctttc gaagatagcg cagagttctt aggtagagtg
    12001 ttcttgcaag agggggaaat cgaggccatc cctcttcagt catcaagcct atgtgggagg
    12061 agatgaaaat tttcccggcc attcttgatg cgaccccgcc tgtcctctaa ccggcagtca
    12121 attgttgtgc agtccgattg ggcagagtgt gaggggctta agcctacggc ccccagcgga
    12181 tgccgaagag caacgcgacg ctggtgttgt cgtttgggac gggcggcgag agagtgatgt
    12241 ggtgcagatt caccatgagg gtcgtggaga acgcgatctt cggtccgact tggtattcca
    12301 aagacatgcc caggggaatg aagtgactgg tgtcgttgcg atcgatcttc ccgggtccac
    12361 tgccgcgatt cagatcggca tgcagaatac ccaatccggc aaagggcacg aggttgaatc
    12421 cactgttcag gcggagatga tatttggcga cgccggccac gccgatttgc gtgagatcac
    12481 cgacgggggt gaagagggcc atcccgccga atgaaaaatt ggggtccatg tagtaatcga
    12541 gaccgaatcc caacgcaaag gtcgtgttgt tcgtcgtgcc ggtccagagc ccgagatcgg
    12601 tcgcaaagcc ccaatgcggc tcctgcggat cggcggattg ggcggcgcaa ggcgaaagaa
    12661 atccgatgaa ggcaaggccg gcgagaaaaa gtcggatacg tcgagcgtat gtcatagtcc
    12721 tacgagctat aggtggtggg tgtatgtagc ataggccctt gctgcggaca agcaagggtg
    12781 accagggcgc ccgcatctta gtattgtcaa tccgtcaggg gctccagtat cctgccctcc
    12841 tgagggagga tgggcatgaa aaaagaatcc atgacaccgg ctgaagccgc ggatgccttg
    12901 tataaactga tgccgcgccg gattaccgta gagatgttga gtgactatgg gattcagggt
    12961 gaggacgcgc atgaagatgc catgacgcgc gaactgctgt cctttacgct ctactgggtc
    13021 ctggcggcta tcaacgcgca cattccgcgg aagtaccgtg acctggtgtt ccatcgcgtg
    13081 atggatctga ttcgcgccga ttgggagacc gagtacaaac tcggggcgct gaaatgggag
    13141 gagtatctcg acgaggtcga agcccgccgc gcccgatatg cgccggtggg tgattacgag
    13201 ggcggcgcta tcgccgcatc cgaagaaatc tcagatctgc ttgaaaccga agggttgatt
    13261 caacccgaag atcggccgaa gttgctggtc ctacttcccg atctcgtgcc gctggataag
    13321 tatcaagaac tcctgagcca gtgtgtgtaa gccgcaccct tcgagctgtt tgccgcggcg
    13381 cctagggcga atgttgtcac aggtagcggt gtgactgcac cttcccgttg gtgtcgagtt
    13441 cgtacagcaa cggcgcgccg gtgggaatgt tgagttccag cacctgttcg cgggtcagct
    13501 gttcgagctg catgaccagc gcccgcagac tgttgccgtg cgcggcgatc aggatggttt
    13561 cccccttgag tacatagggc ttgatccggc tctcgtaata cggcaggacc cgctccgccg
    13621 tatccttcag gctttctcct cccggagggc gcacgtcgta actgcgtcgc cagattttga
    13681 cctgctcgtc tccgaacttc ttagccgtct cggccttgtt cagcccttga agttcgccgt
    13741 acatgcgctc gttcagcgcc ttgtcttttt caacaggaat ggtcgtctgt ccgatcgctt
    13801 cgagaatgaa gcgcagggtt tcgttggcgc gagcgaggac ggaggagaag gcgcgatcga
    13861 aggtgaatcc tgccagcttt ttccccgctg ctttcgcttc ctcgatccct ttcggcgaga
    13921 gggggacgtc tacccagccg gtaaaacggt tttccaaatt ccactgcgac tcgccgtgac
    13981 gaatgagaac cagtttgctc atcgtgcttc gtctccttgt ccgaagggat atgaccctgt
    14041 cgcgacaggt tatgcgattt ccccttcacc ctgtcaagct ctcttgtttc gcacatagag
    14101 cccggtccga gtacctcggt tgcaatcaaa ggtggcagtc agtaccatgc cgccggtttc
    14161 ccctgtaagg ttctcacccg tggaatgtcg cgatgccgga tgtttcccct tcggactggt
    14221 cttccttgca gcaatggtgg caggagatgg gacgccgcct gcaggttgag gatcgtgtcg
    14281 aggcgttttt ccgtcgcgcg atgggggcaa gtcaggccct ggcaggtgcg gtgcaggtcg
    14341 gcggggaggt ggaatatatc ctcttcgtgc tggtgcgcta ctggattccc gtcgtgcttc
    14401 cgcctccagg gcgggaacgg gcttccaagg gtgacccgga ctactggctg gaatccgaac
    14461 agattttgaa ggccgccgca gtacgcttga gggaactaaa gccgctgatc gaactgttga
    14521 cggcaagcaa tccactgagt gccggcgttg ctgagccgca ggctgccaga ccaccgatgg
    14581 tggaggtcga actagccaat atgctgcagg ggattgcgga atcggccgga agctacgggg
    14641 ggccggatta cacctcagtg atcaagacat tcgatcccgt tcccttacgt cagacccagc
    14701 cgtttaagca caataagaaa aatagtgccg agttgtgggt ggtctttctg ttgcgcgagc
    14761 acttccgcag ccttgggctg gggaaggatc gctattggcc attggtggcc ggtctctgcg
    14821 cggctgccgg gatcgccaac cctaacggac agccctacgg gccggacgag ctgaagtcgt
    14881 ggtggcagaa gaactggcct cgcacctata cccagttaga acagacgcag gcggcgcccg
    14941 acctcagcgg agcagcgtat cagcaggatt ttgactggtt cgtgtcctgg atcgagtggc
    15001 agcggcagca gatctcggaa gtgtagcctt ctatcagccc gctgctggtt tagacgtgcg
    15061 tcagactggc ggggtgcggt tttgcaggcg ggcctttcgg tccatccggc gggccctctt
    15121 taaacatcgc gacgaccaat ccaattttca gcacgagcag cccgacgccg acccacacga
    15181 catagggttg tacgccaccc aattcgccta agagctgctc gcgggtttct ccccccgtgg
    15241 cccgctcgct tttaattttg gcgagctgtt ccttgacgtg ccagaaatag atgtagttgg
    15301 cgctgcctcc cgcaatgacc cgccagacga tgtcggcgga caagtcctgg gtgatgtaaa
    15361 aggccatggc cgggccgata gcatagatga gcgcgtacac atacatcttc cggtacagaa
    15421 accagagaaa tggctcgaac acgaaggccg gccagtgcca gctcaatgcg aattgcggtc
    15481 cgcccgggcc tgagaatttt ttgaaggttt ccaggtaccg gtcggcattg gggccgatga
    15541 aggctttcaa cagttctgta tctgtctggg gcgcgtcctg ctctgtctcg ctggcggcag
    15601 cgcgagcctc gagcgcaaaa ggtgccccgc actgatgaca aaagcgggca tcgtcacggt
    15661 tttcttgccg gcattgcgag caggttttca tatcgtcccc ttatcccatc cgccatctct
    15721 tcccgtcaag ggacgcccct gtgaaacccc gagcgatcca cctcgccgcc gcgctgggag
    15781 ctgcgaatag gcgctggttc ggttgctttc acctgtgccc tatgatagct tcgccgactc
    15841 acgatgggag aggtgtgtca tgccgacggg tgagttgcgt ctcaatgccg aacagtacct
    15901 gatgaatacc taccagcgtc agccgatttc gatcgtgcgg gggcgtggct cgaaggtcta
    15961 tgatctggag ggccgggagt acatcgattt cgtggccggc attgcggtga atgtgctcgg
    16021 gcatggacat ccggatctgg tgcttgctat tcagaaacag gcgcagcatc tgctccatac
    16081 ctccaatctg tattataccg agccccaagt gcggctcgcc cagaccctgg tcgaacattc
    16141 gtttgcgcag aaggtgtttt tctgcaacag cggcgcggaa gcgaatgaag cggcgatcaa
    16201 gctggccaga aagtattcgt acgacaaata tggcgcggat cgtcacgaga tcatcacgat
    16261 gacgagctcg tttcacgggc gcacgatggc cacgctgacg gctaccggcc aagacaaagt
    16321 ccaaaaggga tttgctccgt tgatgccggg cttttcctat gtgccgttta atgatctgtc
    16381 agcactcgaa cgagcgatca cgcccaagac tgcggccatc atgctggagc caatccaggc
    16441 cgaaggcgga gtgcatgtgg cggaacgggg ctacctgcag ggcctccgag aattgtgccg
    16501 ggaacgtgac gtcctgctga ttttcgacga ggtgcaaacc ggcatcggcc ggaccggtac
    16561 cctcttttgt tatgagcaat tcgggatgca gccggacatt atgaccctcg cgaaaggcct
    16621 tggcggcgga gtgccgatcg gcgcctgcct ggcgacggac agcgtcgctc gtgccttcgg
    16681 ccccggaacc catgcctcga cgttcggcgg gaaccccttg gcctgtgcgt ccgcgctcgc
    16741 ggtattgcgg gtcttgctgg acggcaagat tctggaccag ggccggcgga tgggcgagta
    16801 tttggcaaaa gggctggcga ccttgaaaga acggcatcgc tgcatcaaag aggtgcgcgg
    16861 catgggcctg ttgcagggca tggaattgga cgtcgacggc aaaacggttg tcgccgattg
    16921 tctgacgcga gggttattga tcaactgcgt tggagagcgg gtgctgcgtt tcgtgcccgc
    16981 gctcatcata acggagcgcg agatcgatcg cctcctggcc tcgctctcac aaattttgag
    17041 tcaacgaacc acttcaaccc attaattgtg tcgtggcgcg tatgttgcgg cgtcttcgtc
    17101 ggtcaactcc ccggaccggc ctcgggaaag attttctgga tttgctctcg attcccaccg
    17161 acgagctgac cggattgctg cgcctcgcgg cccagctgaa agtcaaacaa cggcgcggcg
    17221 tctctcatcc cctgctgcaa ggccgcatgc tagggttgct ctttcagaag ccgtcgaccc
    17281 gaacgcgcgt gtcgttcgag gcgggcatga atcagctcgg cggccaggcg atggtgttgc
    17341 ccatgggcga catccaattg tcccgcggcg agagcattgc cgatacggcc catgtgttgt
    17401 cgcggtacct cgacgcgatt gtcctgcgaa cgtttgatca tgccatcgcg gaagagtggg
    17461 cccgcgaagc cagcattcct gtgattaacg gccttaccga tctgaaccat ccctgccagg
    17521 ccctgtccga tctgttgacg attcaggaga aaaagcgacg gctgaagggc ctcaagctcg
    17581 cctatgtcgg cgatgggaac aatgtcacga attcgctcat cgaagctgcc gccaagatgg
    17641 gtatgacgat cgcggtgggc tgtccgcccg gctatcagcc ggaccggcgt attgtggagc
    17701 tgtctttggc cgaagcggag cggacggggg cggtcattga aatcggctcc gacccctctg
    17761 tggccgtcaa ggatgcggac gtggtctata ccgatgtctg gatcagcatg ggacaggagc
    17821 gggagcaggc caagcgcctc aagacccttg cgccctatca gctcaatgcg cgcctgttga
    17881 aatgcgcgaa gcccgatgcg ttggtgatgc attgcctgcc tgcccatcgc ggcgaagaaa
    17941 tcagcgccga ggtattggat ggcccgcagt cggttgtgtt cgatcaggcc gagaaccggt
    18001 tgcacatgca gaaggcgatt ttggtcaggc tcctcggaaa gaaaaccgca cggaagacgt
    18061 aaggaaaagg agtggtatga gtcagtcgtc gtacaagaaa gtggtgctgg cctattctgg
    18121 cggattggat acctcggtta ttctcaaatg gctggaagaa gtctatggct gcgaagtggt
    18181 ggccttttgc gccgacctcg ggcaaggcga agatttgaag gccatcaaga aaaaggcgca
    18241 gtccctgggt gtgaagaagg tgtatgtcga ggatttgcgg gagacgtttg tcaaggatca
    18301 cgtgtttccc atgttgcgtg ggaatgcgat ctacgaaggg agctatctgt taggcacgtc
    18361 gattgcgcgg ccgctcattg ccaagcggca aatcgagatt gccgccaagg aaggcgctgc
    18421 agcggtctgt cacggcgcga cgggaaaggg caatgaccaa gtccggttcg agctgacata
    18481 catggcgctg catccccaga tcaagattat tgcgccgtgg cgggaatgga cgatgcgctc
    18541 gcggcgggag ttgattgaat atgcggagaa gcacggcatc cccgtcaccg cgaccaaggc
    18601 caagccttac agcatggata tgaacctgtt ccatacgagt tatgaggggg gcattctgga
    18661 agatccgtgg gaggccccgc cggaggaaat tttcgtcatg tcggtttcgc cggaacgcgc
    18721 gccggccaag gcgcgtgaag tcgagattga atatgtcgcc ggcaatccgg tggcggtgga
    18781 cggaaagaag atgagcccgg ccgcactctt ggggcatctg aacaagttgg gcggcgagca
    18841 tggcgtcggt cgcgtcgatc tcgtggaaaa ccgctacgtc ggcatgaagt cacgcggtgt
    18901 ctatgaaacg cccggtggaa ccattctgca tgcagcgcat cggggcttgg aatcgttgac
    18961 gatggatcgg gaagtgctgc acctgcggga tagtctcatt ccgcgctacg ccgaactgat
    19021 ctattacgga tactggtatg cccccgagcg tgaaatgctg caggtagccc ttgatgaggc
    19081 tcaaaaagat gtcacgggca ctgtgcgggt gaagctgtac aaaggcacct gtacggttgt
    19141 tggacggaag tctccgcgct cattgtatcg cttggacatg gcgacgttcg aagaagacga
    19201 tgtgtaccgg cagaaagatg ccgagggatt cattcgtctc aacgcactcc gcttggcgat
    19261 tcgcgcacaa cgtaagaaga ggccgggcag gtaatgggga aatccacacc tgtcggcgct
    19321 cgcgggcgca agtcggcgcg tgctccgcaa cggtccggcc gggccttgcc caaagggaag
    19381 gcctggggtg gccggtttgc cgagcaaacg gaccgtttag tggagcaatt ctcctcatcc
    19441 atcgcctacg accggcgact gtatccctat gacatccaag gcagcatcgc ccactgcaaa
    19501 accctcgagc gggcaggcgt tctgactggc cgcgagtcgg gtcagcttgt gcgaggcttg
    19561 caattcgtga aggtggagct ggatggcggg cggtttccct tctcgccgca ggatgaagac
    19621 attcacatgg cgattgagcg ccgattgacg gagctgatcg gacctgtcgg gggaaaactc
    19681 cacacaggcc gaagccgcaa cgaccaggtg gcgctggatc tgcggttgtt tttgcgcgat
    19741 gtgttgtcca ccttcatgaa gcaggtgcag gaattccgac gggtgctggt gggacaggcg
    19801 cgggcgcacc tcgatgtcgt gatgcctggc tacacacatt tgcagcgtgc gcagccggtg
    19861 cttttggccc atcattttct ggcctatgtc gaaatgctgg accgggatcg gacgcggctg
    19921 cacgattgtc gccaacggct caatgtcatg cccctggggt cgggggctct ggctgggtcg
    19981 aactatccgg tggaccgccg gtatacggcc gcgctgcttg aatttccgtc ggtcacccaa
    20041 aacagccttg acgctgtgtc ggatcgtgat gccgtggtgg aagtgctcag tacattgtcc
    20101 ctgatcatga tgcatctctc gcggttgagt gaggaattga ttc
//
LOCUS       DAOQDS010000066        20122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1031_length_20122_cov_19.644441, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000066 DAOQDS010000000
VERSION     DAOQDS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20122
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1031_length_20122_cov_19.644441"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PK589_14545"
     CDS             complement(<1..1049)
                     /locus_tag="PK589_14545"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HNJ20878.1"
                     /translation="MMTRIVLLVLDGFGIGSLPDAESYGDGGCNTLQRLAAISKGLAL
                     PNLEQLGLGLLGQFQGIRPMAQPEGCYGMLGFTTKGKHSLAGHWELAGYVIEDGPTPL
                     EAFTSELASGLEAALGQKTLGNCRTVHLEPIAEFGAQHQKSGMPIAWIDTAGTIMLAA
                     HEQVIPPEELYRLAREARRRLKATMPIVRVVAHPFTGQPGQFAITERRRDFAVEAPGL
                     TLLDHLSQASQLVFGVGKVGDLFSGRGVTRSVPLFQAHAVMDEVIGMFSKAPRGLIYA
                     NLPIMSPDLQDTLSTLQHVDRRLRDLQECLKVGDVLIITGDHGFDCARPNPGHSREYV
                     PCLVTGPRLARGVNLG"
     gene            complement(1138..1830)
                     /gene="deoC"
                     /locus_tag="PK589_14550"
     CDS             complement(1138..1830)
                     /gene="deoC"
                     /locus_tag="PK589_14550"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNJ20879.1"
                     /translation="MGLVSWNENLPALLDHTVLRPEATKADVLRLCAEAKEQGFVVIF
                     VPPCYVDEAVAAVAGTAVQVGIPIGFPLGGHSTHAKVTEAIEAVAHGARVLDMVINIS
                     RLKSGDYDFVRQDMAAVVRATPGVNHKVILETCLLTREEKITACRLAVEAGMDYVKTS
                     TGFNQAGATVEDVRLMKEAVAGQAKVKASGGIRDWKSTRALLEAGADRIGTSASLKIL
                     SEWRAEQAVMTR"
     gene            complement(1916..2968)
                     /gene="mltG"
                     /locus_tag="PK589_14555"
     CDS             complement(1916..2968)
                     /gene="mltG"
                     /locus_tag="PK589_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNJ20880.1"
                     /translation="MMQKRLIAGLVLATVILLALSGYLVLRWAQSPVASGLPKPPSKI
                     VTIPDGSTFQQVAGMLKSEQLIRSRWAFLLLGRSHDSDRKIRPGEYELDGGMSPQDIL
                     SKLLAGRVVLHPVTIPEGYNLAQIAEVLAGQQVTDAKEFSKLVRDRTFIATLGIEADS
                     LEGYLFPETYSFPRQAKARDVIKAMVDGLHRVWSADLQEQAARMKLSLHQVLTLASVI
                     EKETGSKEERELIAAVFHNRLRKKIPLQSDPTVIYGLPAFDGNIHKRDLSVMSPYNTY
                     RVQGLPPGPIASPGAHSLRAALFPAQASYLYFVSRNDGTHQFSSTLAEHNLAVEKYQK
                     QPFRKRARGSLVTHGA"
     gene            complement(2853..3464)
                     /gene="ruvX"
                     /locus_tag="PK589_14560"
     CDS             complement(2853..3464)
                     /gene="ruvX"
                     /locus_tag="PK589_14560"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNJ20881.1"
                     /translation="MKGQRILAIDHGSKRIGFALSDELGWTAQPLETFHRRNPEADIR
                     HIQELVREHEVGRVLVGLPLRLDGESGPAAKVVEEFIQLLEPALSVPVVTWDERMTTC
                     AAEDLLIAADVGRRKRKGIVDRIAAAILLQSYLASLEKPSTSQEQAISNSHEDDPWSF
                     DEPRVDDAEAIDCGTGSSDGDTLGPLGLSGPALGSKSRRQRAS"
     gene            complement(3469..6099)
                     /gene="alaS"
                     /locus_tag="PK589_14565"
     CDS             complement(3469..6099)
                     /gene="alaS"
                     /locus_tag="PK589_14565"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNJ20882.1"
                     /translation="MSQSVNDLRRAFIRYFEQQGHRAVPSAPLIPQADPTLLFTNAGM
                     NQFKRVFLGEETRAYQRAVTVQKCLRAGGKHNDLENVGYTRRHHTFFEMLGNFSFGDY
                     FKEDAIRFGWEFLTSVVGLSKDRMWITIFREDDEADRLWRKIGVSPSRIVRCGEKDNF
                     WQMADTGPCGPCSELHFDQGPSVPGDDTPNGEGDRVIEIWNLVFMQFNRDSAGTLNPL
                     PKPSIDTGMGLERLTAVAQGRLSNYDSDLFAPLLAAIGRRAGAEYGAVEQSDRSMRVI
                     ADHLRAITFLMADGVLPSNEGRGYVLRRILRRAARHGRLLGITEPFLHELTATVVDQM
                     GEAYHELRPAAGTVAEATRGEEERFIATLDQGLPILNDMLSKVKTSGQHVLQGTEIFK
                     LYDTYGFPMDLIAEACREQGITLDETGFEAAIEEQRTRARKTGGFESETARPALSDVA
                     ARVGTTAFVGYDRLDSEGVVQALLQSDRLIKEAREGDEIEIVLDVTPFYAEGGGQAGD
                     QGVLSGTDGRVEIRETTRPVPTLIVHKGVVTSGSIREGERLQLSVNPRTRKDAARNHT
                     ATHLVHAALRDLLGPHVKQYGSLVAPNRLRFDFAHFRPMSSRDIDEIESIVNEQVRLD
                     QPVQTDVMGVQDAVAGGALAFFGDKYGDRVRVVQIDTFSKELCGGTHCGRTGEIGLFR
                     IVSESGVAAGVRRIECLTGSGALDSLKRLEADVREMSELLKVAPTEVVARTRKLSEQL
                     KDKERELADVKLKMASTSSGDAQAREIKGVQVHAQRTDGLDVNGMRALADQLRDKLRS
                     GVVALGAANDGKVSLLVVVTKDLVGRLKAGELIKEMATEVGGTGGGRPEMAQAGGKNP
                     EGLGPALEKVFGLVQKALEG"
     gene            complement(6096..6542)
                     /locus_tag="PK589_14570"
     CDS             complement(6096..6542)
                     /locus_tag="PK589_14570"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HNJ20883.1"
                     /translation="MDFLTMAIRYLGRAERTASQVQHYVQEKGASRVQGHAVVRELQR
                     RGYLDDQAYATRWAEARLSRRPMGRERLKLELLRRGFEDTVAERALCSAYRSVSEQEL
                     ACQALEGRTTRMRPLQWVRFLRQRGFDDDTIQQVTQVDLETGLDEL"
     gene            complement(6593..7705)
                     /gene="recA"
                     /locus_tag="PK589_14575"
     CDS             complement(6593..7705)
                     /gene="recA"
                     /locus_tag="PK589_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNJ20884.1"
                     /translation="MSEKDEKKRALDLALAQIEKQYGKGAVMKLGTDDRPADVPAISS
                     GSLGLDIALGVGGFPRGRVIEIFGPESSGKTTLTLHAIAEAQKAGGVAAFIDAEHALD
                     LTYAKKLGVHTDDLLVSQPDTGEQALEIAETLVRSGAIDVIVVDSVAALVPRAEIEGE
                     MGDAHMGLQARLMSQALRKLTAAIAKSQTTLIFINQIRMKIGVMFGNPETTTGGNALK
                     FYSSVRLDIRRIESIKDGQDVTGSRVRVKVVKNKMAPPFRQAEFDIMFAEGISKSGEI
                     VDMGVEKRVVEKAGAWYSYKGERLGQGREAVRDFLKTNPAIAKEIEGKVRELAGLPLR
                     GAEKKAEAKEAKEEKPERKVEIRQDEKRGHSARVTS"
     gene            8259..8522
                     /locus_tag="PK589_14580"
     CDS             8259..8522
                     /locus_tag="PK589_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20885.1"
                     /translation="MPQQHMDDHSGQSAVQLHVPAARAPRDLTYDEKKAAEAAFRGEP
                     FNPAWSAAAAVVYEGIVSAMCRMQVASLTDMEAVSVAEECLTR"
     gene            complement(8561..9178)
                     /gene="thpR"
                     /locus_tag="PK589_14585"
     CDS             complement(8561..9178)
                     /gene="thpR"
                     /locus_tag="PK589_14585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNJ20886.1"
                     /translation="MIRAFLAVELPPDFRAALSVIQQDLKRRLEPAVGRQARVSWVQP
                     ASMHLTVRFLGETPQEVIESLHAVVSEVVANHHMIEVPFSRLGTFPRIQQPRVLWAGP
                     LESWEQGSHGQRLQVLYRAVEEVCREAGLAAEVRPFSPHLTLARIKEGERQVGQVLAQ
                     SGALDQPVTIGVLPIKALVMMRSELRPAGPVYSKLWECPLAASAA"
     gene            complement(9175..10458)
                     /locus_tag="PK589_14590"
     CDS             complement(9175..10458)
                     /locus_tag="PK589_14590"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNJ20887.1"
                     /translation="MNKSKAWTAETIAIGSELLLGGRMDTNSLFIADHLATCGVELRY
                     KSIVGDELSDIVGVLKTAARRVRVVIITGGLGPTIDDLTREAVAQATGQRLSRRKTAL
                     DGMIARLAEWGRTPTKAQLRQAMIPAGGEVLTNPVGTAPGFALIWKDTFFAALPGVPR
                     EMEPMIQDGVLPRLQAWIGKHKGIPATPVIRRVLHTSGVPESIVDQRLEGLLPDGTAI
                     QLGIYASPMEVMVSLTGPEKESGPVTIDRLFEDAKTRLGDIMYGEADETMETVVGRLL
                     TDQHLTLALAESCTGGLIGHRLTQIPGSSAYVDRGVVSYSNRAKVELLGVSQDLLDRH
                     GAVSAEVAAAMARGIRERSGVSVGLSVTGIAGPGGATKSKPVGLVYVGLDGGPGENLT
                     KEFRFHGGDRSVIKQRSSQAALDVLRRWLITKGGG"
     gene            complement(10505..11119)
                     /locus_tag="PK589_14595"
     CDS             complement(10505..11119)
                     /locus_tag="PK589_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20888.1"
                     /translation="MRTIRRRCAGFKLALYLFVVAGTGPPALSLAIEVQPTPEQIQAT
                     MERGKTAAAKGASPDQLHEWFGAKEDLTPHGFIMTKLGSLLVMANHLSLRALSPTDQD
                     VAQVMANGNLLVNVVLFGDRVNFAADSYIVLEQAGRTVKPSSVRFDARGERTSVWPQQ
                     PAYRAKVIAQFPYADLDPHAKTTLSVFPSSGGQVTFELDFAHIE"
     gene            11265..11972
                     /locus_tag="PK589_14600"
     CDS             11265..11972
                     /locus_tag="PK589_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HNJ20889.1"
                     /translation="MSSPEQAPRRQYVNFTFYKVDPAWRRLPEDERTRGKQEFLRAVE
                     EYQGKVLVIAYTSVGIRGDCDLMLWRISYELELFQEMSTKILASGLGKYILNPYSYLA
                     ITKRSIYVDNHTHENQESKRLTVVPGKAKYIFVYPFVKTREWFLLTKAARQGMMDEHI
                     EVGHRFPSVKLNTTYSFGLDDQEWVVAFESDKPEDFLDLVMALRETEGSRYTLRDTPI
                     FTCIRKSLKEALDTLGG"
     gene            12081..12743
                     /locus_tag="PK589_14605"
     CDS             12081..12743
                     /locus_tag="PK589_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20890.1"
                     /translation="MSVEAQTHPASDYRAAVYSAILPGLGHLLRGRHKAAMFFGFITI
                     LLIILSLGLGRVSGRAAEVFFFMLLALPWWALQSYDAALGPAASGSDLVRTGRLAWAE
                     GHDIRFLGLLFLVSAANDAIIIAKNPDYLLPFFCTKLDGAAGFVTKALSPFLHTWVGY
                     GFLRIKKWSLLVYLVYAAYGTTNALVNLTCFGPGRIRNTLLIALIAFTSYILWRRRMF
                     QR"
     gene            12847..13059
                     /locus_tag="PK589_14610"
     CDS             12847..13059
                     /locus_tag="PK589_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20891.1"
                     /translation="MAENNAVYAIRHPDGSVTLYIDEEYAIDRGVDPAKLVRVEIPRE
                     LFVSGSIQHIREYVAVYLENSHQGTA"
     gene            13092..14261
                     /locus_tag="PK589_14615"
     CDS             13092..14261
                     /locus_tag="PK589_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20892.1"
                     /translation="MSALLTSELIASTIEQAPIQGRVMLKLLLLQHFDITPEEISYIA
                     ADRPDPRCVAGSKPIHQMVKQDALRDVTNRRDEYRRRVRLRRERLWLQMEAMKGMIAL
                     AEGMIRAATDLLRTRFKLDAEAVNAITQSARTAVPKPAIRSLNRRWDEDEISAESYQE
                     ARLGLEIQAQLRLIEKYRKRLEVSEREWKSINAAPLQDHEIGHIWGIPAGSLAGRKVK
                     HLHQYLHAIQSALQKASAQTVPTTGALDLWKETLAVLGERPVERSIALYDGLERTEEA
                     LVEKLRTFAWGTLGEDLEAKFWLSLVHGASSNAVHSENTRSVFGLQRLLAVLSEIDSS
                     PDALEEELLARTVATPKDVPALTDEAAQPKAEEMGQMQQHVLTSFIGELHNDLQR"
     gene            14364..14819
                     /locus_tag="PK589_14620"
     CDS             14364..14819
                     /locus_tag="PK589_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20893.1"
                     /translation="MYNPPIMQHDHVMIPVRSLARVAGLMLCMLVLPLSIMPVASLFA
                     AGLVEVTELLAHPDHYNHQVVTVSGRVSSFQLATNRDGQPAFGFLLQDTAGTVKVVGL
                     GKADVREGDYVVVEGIFSRLRQAGRAIVYNEIKATSVQSMAKMNPDLVG"
     gene            complement(14816..15292)
                     /locus_tag="PK589_14625"
     CDS             complement(14816..15292)
                     /locus_tag="PK589_14625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNJ20894.1"
                     /translation="MHVTAGDVVSVEYVIRLEDEQVIDTTVGEAPLVYTHGQHEILRG
                     LEAGLEGMAIGAAKRVTVMPVDGYGERHPEGLFEVRRDRVPVEAQRVGAKLETTAPDG
                     RVVFPYVAEIKPEVIVLDLNHPLAGKTLVFDVRVIDIEREGNRVVEEIAPAKKGES"
     gene            complement(15530..16096)
                     /locus_tag="PK589_14630"
     CDS             complement(15530..16096)
                     /locus_tag="PK589_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNJ20895.1"
                     /translation="MPSEVLSVSSRRNFLRTVTVGTALFVANLALPAKARASRLPTGR
                     ISLFNLQTEERLSVTFRTESGTYDQSALDDLNYFLRCHHSNETTTMDVQLIEFVTLVQ
                     QKVGGRRDIHIVSAYRSPEYNEQLIRMGTRAARHSYHVSGQAVDVQIPGVPLRTVREV
                     ALRLGCGGVGYYPRGKFVHLDSGPFRHW"
     gene            complement(16407..>17033)
                     /locus_tag="PK589_14635"
     CDS             complement(16407..>17033)
                     /locus_tag="PK589_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ20896.1"
                     /translation="QDHTFERVGGHQAIEMNVRIIAATNVNLKEAIEKGTFRDDLYYR
                     LGVVHINLPPLRERGEDVSLIAMAFLRQAAAHYNKPLYGFTREALEAMQAYSWPGNVR
                     ELSNRIGRAVVMAEGTHITAADLDLPRQAPPQDDGSISLKVNQQRIETDLIMKAFTLS
                     QGNLSRAAQELGISRSTLYRRLRQYGMERSLEARRFPGLSPRASMTEH"
     assembly_gap    17034..17133
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17134..17600)
                     /locus_tag="PK589_14640"
     CDS             complement(<17134..17600)
                     /locus_tag="PK589_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ20897.1"
                     /translation="MAKHSVVIVGPDQSTVSAAQALIQDEFEVSTAMWTTDTLLPIHE
                     QASPVMLLDIDSPSQPSAEEGLNVLQKLRQTGYSGKVIAYTGRPERSVAVRAVQCGAC
                     DVLAKSLDPVQLLQSIERVARVADLEQEARGAMVVGGPEEFSGMLGMSASINR"
     gene            complement(17711..18103)
                     /locus_tag="PK589_14645"
     CDS             complement(17711..18103)
                     /locus_tag="PK589_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20898.1"
                     /translation="MGQSSTGGNGARSGKRPTVAVIYEDVTSGYRYADWFALHGYQAT
                     MTSAVPHLEHHLQELRPDVIVVGLSSCSTPLQTAVPRLKRTCPQVPIIAMLDRTAQDA
                     NGTLPALAKAFGGDVFMCDSLDPPSTVS"
     gene            complement(18494..>18694)
                     /locus_tag="PK589_14650"
     CDS             complement(18494..>18694)
                     /locus_tag="PK589_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNJ20899.1"
                     /translation="PHERLSDREYEVLCLIASGQTVSDIAESIHLSVTTISTYRARIL
                     DKMNLKNNAELTRYALQHGLVV"
     assembly_gap    18695..18794
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18908..>20122)
                     /locus_tag="PK589_14655"
     CDS             complement(18908..>20122)
                     /locus_tag="PK589_14655"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNJ20900.1"
                     /translation="ELVGRRKQGDEFPLELSVTSWKGKSDLFFTIIMRDISERRTAEE
                     QLDRLHYHNQVVLNSAGEGIYGVDREGRLTFVNPAAAKMLGWEADAMIGRSLSALVFS
                     PDLAERPFGALSCPIVETIRDGEVREQVDSAFWRKDGTSFPVEYVSTPISERGELVGA
                     VVVFKDTTDRKRAEAQLQDSLRRLRKLSGRMEGIREEERGRIARELHDELGVGLTCLK
                     IDLSRLGGLLGERLVSGDRAKVDEKIRGMKEQVDSTITSVQRIVAELRPGVLDDLGLV
                     AAIEWQCRDFQRRTGITCHCVVSHEDLRVDPEQATAVFRICQEALTNVTRHAQATDVH
                     VRLEEQNVGLLLQVRDNGRGIPQDRLADARSFGLLGMRERAGLLGGDVQIETLEGNGT
                     TITLQLPREGKG"
BASE COUNT         4112 a         6319 c         5346 g         4145 t
ORIGIN      
        1 ccgaggttga cgcctctggc aaggcgtggt cccgtcacca ggcaagggac atattcgcgt
       61 gagtgtccgg gattcggtct ggcgcagtcg aatccgtggt cgcccgtgat gatcaggaca
      121 tcgccgactt tgagacactc ttgcaagtcg cgcaaccggc gatcgacgtg ttgcagcgtg
      181 gacagggtgt cctgcagatc ggggctcatg atagggagat tcgcatagat caatccgcga
      241 ggcgctttgc tgaacatgcc gatgacttcg tccatcacgg catgcgcctg aaacaagggt
      301 accgatctgg tgaccccgcg cccgctgaaa aggtcgccga ctttacccac cccgaatacc
      361 agctggctgg cttggctcag gtgatccagc agtgtgaggc ccggtgcttc cacggcaaaa
      421 tcccggcgac gttccgtaat agcaaattgt cccggctggc cggtaaacgg atgggccacc
      481 acgcgaacga tcggcatcgt cgccttgagc cggcggcgag cctcgcgcgc caggcgatac
      541 aactcctcag gcgggatgac ctgttcgtgg gcggccagca tgatggtccc ggcggtatcg
      601 atccacgcga tgggcatgcc tgacttctga tgctgcgcgc cgaactctgc aatgggctcc
      661 aggtgaacgg tgcgacagtt ccccagcgtc ttttgtccca atgcggcttc gagaccggag
      721 gccagctcgc tcgtgaacgc ctcgagaggg gtcggaccat cttcgatgac atacccagcc
      781 aattcccaat gtccggccag cgagtgttta cccttggtcg taaaccccag catgccgtag
      841 cagccctccg gttgagccat ggggcggata ccttgaaatt ggcccaggag accgaggccg
      901 agttgttcca gattcgggag ggcaagtcct ttggaaatcg cagccagccg ctgcagcgta
      961 ttgcagccgc catcgccata cgattccgca tcgggcaaag acccgatgcc aaatccatct
     1021 aagaccagca ggacgattcg agtcatcatc gaggcactat atcaaatccg ctgaaacgag
     1081 caagccaatc tcaccgtccg agcacagtga cggccgttcc ttcgtaccca gtgggactca
     1141 gcgagtcatg acggcctgtt ccgcacgcca ctcgctcagg attttcagac tggcgctggt
     1201 gccgatccga tccgccccag cctccagtaa cgctcgtgtc gacttccaat cccgaatgcc
     1261 gccagaagcc tttaccttcg cctgtccggc cacggcctct ttcatgagcc gcacatcttc
     1321 caccgtggcg ccggcttggt tgaatcccgt ggaggtcttc acatagtcca tacccgcttc
     1381 aacggctagg cggcacgccg taattttttc ttcgcgtgtg agcaaacagg tttccagaat
     1441 gaccttatgg ttgactccag gcgtggcgcg cacgacggcc gccatatcct gacgtacgaa
     1501 atcatagtct ccggatttca ggcggctgat gttgatgacc atgtccaaca ctcgcgcgcc
     1561 atgcgccacc gcttcgatcg cctcggtcac tttcgcatgg gtcgaatggc caccgagagg
     1621 gaacccgatc ggaatgccca cttgcacggc ggttccggcc acggccgcaa ccgcctcatc
     1681 gacataacag ggcggcacaa aaatcacgac aaatccctgc tccttcgcct cggcacacag
     1741 ccgcaagacg tcggcctttg tcgcctcggg acgcaatacc gtgtgatcca gcagagcagg
     1801 taaattctca ttccacgaca caagtcccat gcgcgattcc tttcagatga tgaattttga
     1861 tggatgtaat agaccgtcac gaggcgatgc ggcgtggagt aaggcaccaa taagatcacg
     1921 cgccgtgagt caccaggctt cccctggccc gcttgcggaa cggctgcttc tgatactttt
     1981 ccacggcgag attatgttcg gccagggttg aggagaactg atgggtgcca tcgttgcgcg
     2041 agacgaaata gagataggac gcctgcgccg gaaacagtgc tgcacgaagc gagtgagctc
     2101 ctggactggc gatcgggccg ggcggaagtc cctgcacacg atacgtattg taggggctca
     2161 tgacggacag gtcgcgttta tgaatattgc cgtcgaacgc cgggagccca tagatgaccg
     2221 tcggatcgct ctggagggga atcttctttc gcaaccggtt gtgaaacacc gccgcaatca
     2281 attcccgctc ttccttggag ccggtctctt tttcgatgac tgatgccagc gtcaagactt
     2341 gatggagcga caacttcatg cgcgcggcct gttcctgcaa gtcggcgctc catacgcgat
     2401 ggagcccgtc caccatggcc ttgatgacat cacgggcctt ggcttggcga ggaaaggaat
     2461 aggtctccgg aaacaagtag ccttccagcg agtccgcttc aatccctagc gtcgcaatga
     2521 acgtgcgatc gcgcaccagc ttcgagaatt ccttcgcatc cgtcacctgc tgtcccgcca
     2581 gcacctcggc gatttgcgca agattgtatc cttcggggat cgtgaccgga tgcaatacga
     2641 cccggcccgc caacagctta ctcagaatgt cctgaggcga catgccgcca tcgagttcgt
     2701 attcgccggg gcgaattttg cgatcactgt cgtgcgaccg gccaagcagg agaaacgccc
     2761 agcggctgcg aatcagctgc tcgctcttca acatgcccgc aacctgctga aaggtactgc
     2821 cgtcaggaat cgtgacgatc ttggagggtg gcttaggaag cccgctggcg acgggacttt
     2881 gagcccagcg caggaccaga tagcccgaga gggccaagag tatcaccgtc gctagaacca
     2941 gtcccgcaat caatcgcttc tgcatcatcg actcgtggtt cgtcaaaaga ccagggatcg
     3001 tcttcgtggg aattgcttat ggcctgctct tggctcgtcg acggcttctc caaactcgcg
     3061 aggtaactct ggaggagaat cgcggcagcg atgcgatcga cgatgccttt ccgtttgcgg
     3121 cggccgacat cggcggcgat caataagtcc tccgccgcac aggtcgtcat ccgttcgtcc
     3181 caggttacca ccggaaccga caaggccggt tccagcagtt ggatgaactc ctcgacgact
     3241 tttgcggcgg ggccactttc cccgtccaat cgaagcggta agccgacgag cacccggccg
     3301 acctcgtgct cgcgcaccaa ctcctgaata tgtcgaatat cggcctcggg attgcgtcga
     3361 tgaaacgttt ccaacggctg ggcggtccac ccgagctcat cgctcagagc aaagccgata
     3421 cgtttggatc cgtgatcgat cgcaagaatc cgctggcctt tcatcagtct acccttccaa
     3481 ggccttttgg accaacccaa aaactttttc gagcgccggt cccagtcctt cgggattttt
     3541 tccgccggcc tgcgccatct cagggcgacc accgcctgtc ccgccgactt cggtcgccat
     3601 ctccttgatc aattcacccg ccttcaaacg gccgaccaga tccttcgtca ccaccaccag
     3661 caacgagacc ttgccgtcgt tcgccgcgcc caaagcgaca acaccgcttc ggagcttgtc
     3721 ccgtagttga tcggcgagcg cccgcatgcc gtttacatcg aggccatccg tacgttgcgc
     3781 gtgcacctgg acgcccttga tctctcgtgc ctgcgcgtca ccggacgatg tgctcgccat
     3841 tttcagcttc acgtctgcaa gttcgcgttc cttgtccttc agctgttcgc tcaacttccg
     3901 ggtgcgcgcc acaacctccg tcggcgcgac tttcagaagt tcagacatct ctcgcacatc
     3961 cgcttccagg cgcttgaggg aatccaaggc accactcccc gtcaaacact cgatgcggcg
     4021 caccccggcg gctactcccg attcagagac gatccggaac aatccgattt cccccgtacg
     4081 cccacaatga gtgcccccgc acaattcctt gctgaaggtg tcgatctgga ccactcgcac
     4141 ccgatcgcca tacttgtccc caaagaaggc caatgccccg cccgccacgg catcctgcac
     4201 gcccatcaca tcggtttgca ccggctgatc aaggcggacc tgctcattaa cgatcgattc
     4261 gatctcgtcg atgtcacggg acgacatggg ccggaaatgg gcaaagtcga accgcagacg
     4321 attcggtgcc acgagcgagc cgtactgttt gacgtgcggc ccaagtagat cgcgcaacgc
     4381 ggcatgcact aaatgggtag cggtatggtt acgcgccgca tctttcctgg tccgaggatt
     4441 caccgaaagc tgcagccgtt cgccttcacg aatagaaccg gatgtcacca cgcccttatg
     4501 gacaatcaac gtcggcactg gtctggtggt ctcgcgaatc tccacgcggc cgtcggtccc
     4561 gcttaacaca ccttgatcgc cggcctggcc gccgccttct gcataaaatg gcgtgacgtc
     4621 gagcacgatc tcgatttcgt cgccttcccg ggcttccttg atcaagcgat cactctgaag
     4681 gagcgcctgc accacacctt cggaatccaa gcggtcatag ccgacaaatg cggtggttcc
     4741 gacacgcgcc gccacgtcgc tcagggccgg cctcgccgtt tcgctctcga agccccccgt
     4801 ttttctggcg cgcgtccgct gctcttcgat ggccgcttca aagccggttt cgtcgagggt
     4861 gattccctgt tcacggcagg cctccgcgat gaggtccatc ggaaacccgt acgtgtcgta
     4921 gagtttgaaa atttccgtcc cctgcaacac atgctgcccc gacgtcttca ccttcgacag
     4981 catgtcgttc aggatcggca acccttgatc cagtgtggcg ataaaccgct cctcctcacc
     5041 gcgcgtcgct tcagccaccg tcccggccgc cggccgcaat tcgtgatagg cctcgcccat
     5101 ttggtccacg accgtcgccg tcaattcgtg caggaacggc tcggtgatac cgagcaaccg
     5161 tccgtgacgg gccgctcgcc gaagaattcg ccgaagcaca tagccccgcc cttcattcga
     5221 cggcaggaca ccgtcagcca tcaggaacgt aatggctcga aggtggtcgg caatgacccg
     5281 catggaccga tccgactgct ccaccgctcc gtactcagca ccggctcgcc gtccaatcgc
     5341 ggccagcaat ggcgcgaaca aatcactgtc gtaattgctg agccggccct gcgcgaccgc
     5401 cgtgagtcgc tccagtccca tgccggtgtc gatgctcggc tttggaagcg gattgagcgt
     5461 cccggcgcta tcgcgattga attgcatgaa caccaggttc cagatctcaa tgacccggtc
     5521 gccttcaccg ttcggcgtat catcgcccgg caccgacggc ccctgatcga agtgcagttc
     5581 cgagcaaggc ccgcagggac ccgtgtcggc catctgccag aaattgtcct tctcgccaca
     5641 acgcacgata cggctcgggg acacgccgat cttccgccac agccgatcgg cttcgtcgtc
     5701 ttcgcgaaag atcgtgatcc acatccggtc tttcgacagc ccgaccaccg aggtcagaaa
     5761 ttcccagccg aagcggatgg cgtcttcctt gaaataatcg ccgaaggaaa agttgccgag
     5821 catttcgaag aacgtatgat gccgcctggt gtagccgaca ttttcaaggt cattgtgctt
     5881 gccgccggca cgcagacact tctgcacggt cacggcccgc tgataggcgc gcgtttcttc
     5941 acccaggaac acccgtttga attgattcat cccggcgttc gtaaacagca acgtgggatc
     6001 cgcctgtgga atcaacggcg cgctcggcac cgctcgatgg ccctgctgct caaagtaccg
     6061 gatgaaggct cgccgcagat cgtttacact ttggctcata actcgtctaa ccccgtctcc
     6121 aagtcgactt gcgtgacttg ctgaatggtg tcgtcatcaa acccgcgttg ccgcaaaaat
     6181 ctcacccact gtagtggacg catcctcgtc gttcgtcctt ctaacgcctg gcacgccaat
     6241 tcctgttcgg aaaccgaccg gtaagcactg cacaaggccc gctccgccac cgtatcttcg
     6301 aagcctcgac gaaggagttc cagcttcagt cgttcacgtc ccatcgggcg tcgcgagagc
     6361 cgggcttccg cccaccgggt ggcataggct tgatcgtcga gatacccgcg ccgctgtaac
     6421 tcacggacaa ccgcgtgccc ctgcacccgg ctcgcccctt tttcttgaac gtagtgctgg
     6481 acctgagagg ctgtccgctc agcacggcca aggtaccgga tcgccattgt caggaaatcc
     6541 atcggcgact ccctgcgtcg tggtacacca cgtccagccg acatcctgct gcctatgatg
     6601 tcactctcgc actgtggccg cgcttctcgt cctggcgaat ctcaaccttg cgctcgggtt
     6661 tctcttcttt tgcctctttg gcctcggctt tcttctcggc cccgcgcaaa ggcagcccgg
     6721 ctagttcgcg caccttgcct tcgatctcct tggcgatggc cgggttggtc ttgagaaagt
     6781 cgcggacggc ttcacgcccc tggcccaatc gctcgccctt ataggagtac caggcgccgg
     6841 ccttctccac cacgcgcttc tcgactccca tatcgacaat ctcgcccgat ttggaaattc
     6901 cctcggcgaa catgatgtcg aattccgcct gtctgaacgg cggggccatc ttgttcttga
     6961 ccaccttgac gcggacgcgg cttccggtga catcctgccc atccttgatc gactcaatcc
     7021 gacgaatgtc gaggcgcacg gacgaataaa acttcaaggc gttgccgccc gtcgtggttt
     7081 ccggattgcc gaacatcaca ccgattttca tgcggatctg gttaatgaag atcagggtcg
     7141 tctgcgattt ggcgatggca gccgtcagtt tacgcaaggc ctgggacatc aaccgtgcct
     7201 gcaagcccat atgggcatca cccatttcgc cttcgatctc agctcgaggc accagcgccg
     7261 cgacggagtc cacgacaatc acgtcaatgg cgccgctgcg gacgagggtc tccgcaatct
     7321 ccagcgcctg ctcgccggta tccggctgcg acacgagcaa gtcgtcggtg tgtacgccca
     7381 acttcttggc ataggtcaaa tccagcgcat gttcggcgtc gatgaaggcc gctacgccac
     7441 ccgccttctg tgcctcggcg atcgcatgca acgtcagggt ggttttaccg gacgactccg
     7501 gtccaaaaat ctcgatgacg cgcccacgag ggaatccccc tacgccgagc gcaatatcca
     7561 atcccaagga tccgctcgag atcgccggta cgtcggccgg tcgatcatcg gtccccagct
     7621 tcatcaccgc gcccttgccg tattgttttt caatctgagc cagggccagg tccaacgcgc
     7681 gtttcttttc atctttttca gacattcata actccttctc gcgacatgac tcgtgaagtg
     7741 tgcctgtctc gcatgccgtg ggagacaacg caccagccgg ccatgagttg accacgcgcc
     7801 agactcagac caggaacgca gacagaacag cggattatac cggacttaga aggaagaagc
     7861 ctagcccgca cgggcctgcg atccatccaa tctcgtcaat caagacggga tggagcccct
     7921 tcgtgaatct cgcgaaacca tgcatcgaaa tcccctgatg ctccatacct gcttccggca
     7981 tcgacgtcaa aaaacccgca gccttccgtc atagctgcca cgtctccgac gtgagagggt
     8041 attggtcttc gcccgccgtg catcgcgagt ccttcctggc gacctgacgc gtaagtatat
     8101 gaaaatgcgt ttgagactga agggagctgg ccaaagagcg gacggctaaa gtcatagcgc
     8161 ggacgcgccg acattcttgg tgagggacaa tgcgcttggc accaagcttg ctcctacctg
     8221 gagtattcgt tcacctctta cggatgcagg aggcccccat gccacagcaa cacatggacg
     8281 atcattcagg acaatctgcg gttcaattgc atgtgcccgc cgctcgcgcg ccacgcgact
     8341 tgacctatga cgaaaaaaaa gccgccgagg cggcctttcg tggcgaaccg ttcaatcccg
     8401 cctggtctgc cgcggcagca gtcgtgtatg aagggatcgt ctcagcgatg tgtcgcatgc
     8461 aggtcgcctc actgactgat atggaggccg tttctgtcgc tgaggaatgt ctcacgcgat
     8521 aaatgactcg cgcacgaggt catgcggcca gacgtgtcgc tcaggctgcc gaagctgcca
     8581 gcggacattc ccacagcttc gaatagaccg gcccggcggg acgcaattcg cttctcatca
     8641 tgacgagcgc cttgatcggc aggacaccga tcgtcaccgg ttgatccaac gctccgcttt
     8701 gagccaggac ctgtccaacc tgtcgttccc cttcttttat acgtgctagc gtcagatgcg
     8761 gactgaacgg ccgtacctcc gcagccaaac cggcctcccg acatacctcc tccaccgccc
     8821 gatatagtac ctgcaagcgc tgcccgtggc ttccctgctc ccaactttcc agcggtccag
     8881 cccacagaac tctcggctgt tggattcgag ggaacgttcc gagccgggaa aagggcactt
     8941 caatcatgtg atgattggcg actacttcgg aaaccactgc atgaagggat tcgatcacct
     9001 cttgcggcgt ctcgcccaga aacctcactg tcagatgcat ggaggctggt tgtacccaac
     9061 tgacacgcgc ctgccgtccg accgctggct ccagccgtcg cttgagatcc tgctggataa
     9121 ccgatagggc cgcgcgaaaa tccggaggca attcgacggc cagaaacgcc ctgatcatcc
     9181 gccacctttc gtgatcaacc agcgacggag aacatccagc gcggcctgcg aggagcgctg
     9241 cttgatgacg ctccggtcgc cgccatgaaa acggaattcc ttcgtcaggt tctcgcccgg
     9301 tcccccgtcg agccccacat agacaagtcc caccggcttg ctcttcgtgg cgcctcccgg
     9361 cccggcaatg cccgtgacac tcagcccaac tgagacgccg cttcgttcac gaatgcctcg
     9421 cgccatggcg gccgccactt cggcactgac cgcgccatgc cggtcgagga gatcctgaga
     9481 gacgcccaac aattccacct tcgcgcgatt gctgtaactc acaacgcccc gatccacgta
     9541 ggctgacgat ccgggtatct gagtcagccg atggccgatc aatccgccgg tgcacgattc
     9601 ggccaaggcc agtgtcaggt gttgatcggt gagtaatcgc ccgaccaccg tttccatcgt
     9661 ctcgtccgcc tccccataca taatgtctcc gagccgggtc ttcgcgtctt caaacaggcg
     9721 gtcgatcgtc acaggccccg actccttctc cggccctgtg agcgacacca tcacttccat
     9781 gggggaagca taaataccga gctgaatcgc cgtcccgtcc ggaagcaatc cctccaatcg
     9841 ctgatccaca atggattccg gcacgccgga ggtgtgcagc acccgccgaa tgacgggagt
     9901 ggcagggatt cccttgtgct taccgatcca ggcctgcagg cgaggcagga ctccgtcttg
     9961 gatcatcggc tccatctcac gtggcactcc gggtaaggct gcaaagaacg tgtctttcca
    10021 gatcaaggcg aaccccggcg ccgtaccaac cggattggtc agcacttccc cgcctgcagg
    10081 aatcatcgcc tgtcgcaact gcgccttcgt cggcgttcgc ccccactcgg ccaaacgagc
    10141 gatcatccca tcaagtgctg tctttcgtcg gctcaggcgt tgtcccgtcg cctgcgcaac
    10201 cgcctcacgc gtgaggtcgt cgatcgtcgg tcccaatcca cccgtgatga tcaccacgcg
    10261 gacgcgccgg gccgccgttt tgagcacccc cacgatatcg gacaactcat cgccgacaat
    10321 cgacttgtat cgaagctcca caccacacgt cgccagatga tcagcgataa acagggagtt
    10381 tgtatccatt ctgccaccga gcaataactc agacccgatg gcgatggttt cagccgtcca
    10441 ggctttggac ttgttcatgc ggaatcaacc tttatagttt gaaaggcgtg aaggaatgtt
    10501 cacgttattc gatatgggca aagtccaatt cgaaggttac ctgcccgccg ctggatggaa
    10561 acacgctcaa ggttgtcttc gcatgcggat cgagatcggc gtagggaaat tgggcaatca
    10621 ccttcgcgcg ataggcaggc tgttggggcc agacggatgt ccgttcgccc cgtgcatcaa
    10681 atcgcaccga ggagggtttg accgtccgcc ctgcctgctc caacacaatg taactgtcgg
    10741 cggcgaagtt cacgcggtcg ccgaacagca cgacattcac cagaaggttg ccgttcgcca
    10801 tcacctgcgc aacgtcctga tcggtgggag acagcgcccg caacgacaag tggttggcca
    10861 tgaccagcag actgcccagc ttggtcatga tgaatccgtg cggagtcaga tcctctttgg
    10921 cgccaaacca ttcatgcagc tgatccggag acgcaccctt ggcagcggcc gttttccccc
    10981 gttccatcgt ggcttgaatt tgttcgggag tcggctgcac ttcgatggcg agcgacagag
    11041 ccggggggcc cgtccctgcg acgacgaaca ggtagagcgc gagtttgaaa ccggcacacc
    11101 gtcgccgaat ggtacgcatg atgaggaggc agtaccagat cgataggggc ctgtcaattc
    11161 gctgccgccg caccccgcca tcggttgaca gcctaggaga cgaaatgcta aagagctaat
    11221 tcttattctt tcacattatc aagaagttgc ctggagggtt cctcatgtcc agccccgaac
    11281 aagcgccccg ccgtcagtac gtgaatttca ccttctacaa agttgatccg gcatggcgtc
    11341 ggcttcctga agatgagcgc acgcgcggaa aacaggagtt cctgcgtgcc gtggaggaat
    11401 accagggaaa agtgctcgtg atcgcgtaca cgagcgtcgg tattcgcggc gactgcgacc
    11461 tcatgctttg gcgtatcagc tatgaactcg aattgttcca ggaaatgagc accaagatcc
    11521 tggcgtccgg attggggaag tacattctca atccgtactc gtacctcgca atcaccaaac
    11581 gctccattta tgtcgacaat cacacccacg agaatcagga aagcaaacgg ctgacggttg
    11641 tgccgggcaa ggcgaagtac atcttcgtgt atccgttcgt gaagactcgc gaatggttcc
    11701 tgctcaccaa ggccgcgcga cagggcatga tggacgaaca cattgaagtc ggccatcgct
    11761 tcccctcggt caaactgaat accacgtact ccttcggtct cgacgaccag gaatgggttg
    11821 tggcgtttga gagcgacaag ccggaagatt tcctggatct cgtcatggcg ctccgggaaa
    11881 cggaaggcag ccggtatacc ctacgcgaca caccgatctt cacctgcatc cgcaagagcc
    11941 tgaaggaagc cttagacacg ctcggcggtt aaaatcgatt gacactccct gacggttcgc
    12001 acggccttcg atccagttga tcgagggccg tgtacgttct accccactta tccattaagc
    12061 cactgcacct ccgcagatcc atgtcagttg aagctcaaac tcacccggcc tccgattacc
    12121 gcgcagccgt gtactcagcc attctccccg gactcggtca cctgctccgc ggacggcata
    12181 aagccgcgat gttttttggc ttcatcacga ttctcctcat catccttagt ctgggcctgg
    12241 gacgcgtatc gggtcgagcg gcggaagtct tcttcttcat gctcctcgcc cttccctggt
    12301 gggcactgca gagttacgat gccgcactcg gaccggccgc ctcgggctcg gacctcgtgc
    12361 gaaccggccg cctggcatgg gcagaggggc atgatatccg gttccttggc ctcctctttc
    12421 tggtcagcgc cgccaacgat gcgattatca tcgccaagaa ccccgactat ctcttgccat
    12481 ttttctgtac aaagttggac ggagctgccg gcttcgtcac caaggccctt tccccatttc
    12541 tccatacctg ggtggggtac ggctttctgc gcatcaagaa gtggtcactc ctggtgtatt
    12601 tggtctatgc ggcctacgga accaccaatg ccctcgtcaa tttaacctgt ttcggaccgg
    12661 gtcgaattcg gaacactctg ctgatcgccc tcattgcctt taccagctac atcctctggc
    12721 gacgacggat gttccagcgc tgaccagatc cgctcggcag ttgaagatcg cattttgaca
    12781 ctcggctcta ggctgtgtta cctgtaatag cgataccgtt ccccatcctc agatggagca
    12841 gctcctatgg ccgaaaacaa tgcggtctat gcgattcgcc accctgacgg ttctgtgacc
    12901 ctgtatatcg acgaagaata tgccatcgat cggggcgtcg accccgccaa gctggtgcgg
    12961 gtcgaaatcc cgcgcgaatt gttcgtcagc ggcagcatcc aacacattcg ggaatacgtc
    13021 gcggtctacc ttgaaaatag ccatcagggc acggcctgag cgaacctttt acctcgcaga
    13081 gaggccacac catgtccgcg ttactcacct cggaacttat cgcctccacc atcgaacaag
    13141 caccgattca gggcagggtc atgctcaagc tgctgctctt gcagcatttt gacatcacgc
    13201 ccgaggaaat cagctatatc gccgccgacc gaccggatcc tcgctgcgtg gccggctcca
    13261 aaccgatcca ccagatggtc aagcaggatg cgttgcggga tgtgaccaat cgcagagatg
    13321 agtatcgccg ccgagttcgt ctgcgtcggg agcgtctctg gctgcagatg gaagccatga
    13381 agggcatgat cgccctggct gaaggcatga ttcgggcagc gaccgatctg ctgcgcacgc
    13441 gtttcaagct cgacgctgaa gccgtgaacg ccattacgca aagcgcccgc acggcagtcc
    13501 ctaagccggc gattcgatcg ttgaaccgca ggtgggacga ggatgagatc tctgctgagt
    13561 cctatcagga ggccaggctt ggtctggaaa ttcaggctca actccgcttg attgagaagt
    13621 acaggaagcg cctggaagtc tcggagcggg aatggaaatc gatcaacgcc gctccactgc
    13681 aggatcacga gattggccat atctggggga ttccggcagg gagcctggct ggccgcaagg
    13741 tgaaacatct ccaccagtat cttcacgcca ttcaatccgc cttgcaaaag gcgtcagccc
    13801 agacagtccc gacgacggga gcacttgatc tctggaaaga aacgcttgcc gtcttaggcg
    13861 aacggccggt tgaacgatct atcgcgctgt atgatggcct agaacgaact gaggaagccc
    13921 tggtggaaaa actccggacc ttcgcctggg gcaccctggg ggaggacctg gaggcaaagt
    13981 tctggttgtc actcgttcat ggtgccagct ccaacgccgt gcactctgaa aacacgcgct
    14041 ccgtcttcgg cttgcaacgg ctcctggcag tcctgtcgga gattgattcc agcccggacg
    14101 cccttgaaga agaactcctg gctcgtacag tggcgacccc gaaggatgtc cctgcactga
    14161 ccgacgaagc agcacagcca aaggcggaag agatggggca aatgcagcag cacgtgctga
    14221 ccagtttcat cggggagtta cacaacgacc ttcaacgcta acgcactgtc gacagctgca
    14281 tggtcgattc tggaacatcg gctggtttcc caccgacccg gcaagatttc tttttcaacc
    14341 ggcttctcag cggggagagt attttgtata atccacctat catgcagcac gatcatgtca
    14401 tgattcctgt ccggtcactg gcgagagtcg cgggtctgat gttgtgcatg ctggtcttgc
    14461 ccctctcgat catgccggtg gcttcgctct tcgctgcggg tctcgttgaa gtcacggaac
    14521 tgttggctca tcccgatcat tacaatcatc aggtcgtaac cgtcagtgga cgcgtaagca
    14581 gttttcagct tgccacgaat cgcgacggtc aaccggcatt cggatttctt ttgcaggaca
    14641 cggcaggaac ggtgaaagta gtgggtcttg gaaaggcgga tgttcgagaa ggtgactacg
    14701 tcgtggtcga aggaatcttc agccgcctgc gccaagccgg tcgcgccatc gtctacaacg
    14761 aaatcaaagc cacctcggtt caatccatgg caaagatgaa cccggacctg gtcggctaac
    14821 tttccccctt ctttgccggt gctatctcct ctacaacccg gttgccttct ctctcgatat
    14881 cgatcactct gacatcgaag accaaggtct tccccgcaag cggatggttg aggtccagga
    14941 caatgacctc cggcttgatc tcggccacat agggaaacac aaccctcccg tcaggagccg
    15001 tcgtttccag cttcgcccca acgcgctgag cctccactgg cacacgatcc cttcggactt
    15061 cgaatagccc ctccggatgt ctctcgccat aaccatccac gggcataaca gtcacgcgtt
    15121 ttgcagcacc gattgccatg ccctcgagtc ctgcttcgag cccgcgcaag atctcatgct
    15181 gtccgtgggt gtagaccaac ggagcctcac cgacggttgt gtcgatgacc tgctcatctt
    15241 cgaggcgaat gacgtattcg actgagacga cgtctcctgc cgtgacgtgc atgtgccagt
    15301 tcctttttcc acagtctttg cgacggtgtg atcaattgcc cagactcgtg cgaaacattg
    15361 tgctcaggtt cgtctgcaac ggcgatggtc gtcgctaaga tggttccgac ttcgatcagc
    15421 ctatcacggc aatcgaccat ggtcatgcga attccggaag ctcagaggag cttcgcgaaa
    15481 ctctgtccgg attactcaag aaggatcgaa ggaggaatgg agggactgcc taccaatgcc
    15541 ggaagggtcc cgaatcgagg tgcacaaact ttccgcgcgg atagtacccg actccgccac
    15601 agccaagtcg caacgcgact tcccgaacag tccgcaaagg cactccggga atctggacat
    15661 caaccgcctg tcccgacaca tgataactat ggcgtgccgc gcgagtgccc attctgatga
    15721 gctgttcgtt atattcaggc gaacggtagg cggatacgat atgaatgtcc cggcggccac
    15781 cgaccttctg ttgaacgagt gtaacgaatt cgatgagttg cacatccatc gtcgtcgtct
    15841 cattcgaatg gtgacaccgc aaaaaataat tgaggtcgtc gagcgcagac tgatcgtatg
    15901 ttccgctctc tgtccggaac gtgactgaca agcgttcttc ggtctgcaga ttgaacaagc
    15961 tgatgcgtcc tgtcgggagt cgcgacgctc gcgcttttgc aggaagagcc aggttggcaa
    16021 cgaatagcgc cgtgcctacg gtgaccgtac gcaaaaaatt ccgtcgactc gacacagaaa
    16081 gaacctcgga tggcatcaat tcgtctcctc gcgaaacgtg cgagtcatct gtcgtgggca
    16141 ctctcgcatc acgccgcaat gtctgttgac agagacggtt cgcacacact ggcggacgca
    16201 aactgcacgg caacacaggc gctatgcaaa taggctgacg atacctcggg aggaaccagg
    16261 ccagcagaga ccggaagact cttcttctta acaaatacca aagaagtggt caaccaattt
    16321 acactcgcga gcggcgtatc cctgctgccg cagcgtcgct cgcctcgttc gcatgacacc
    16381 ataccgcagc agacacgtcc gagagatcaa tgctccgtca tcgaagcgcg cggcgagaga
    16441 ccgggaaacc ggcgggcctc aagagatcgt tccattccat attggcgaag tcgccggtac
    16501 aacgtggagc ggctaatgcc gagctcctgg gcggcccggc tcaagttccc ctgtgagagg
    16561 gtgaacgctt tcatgatcag gtccgtttcg atccgctgct gattgacctt cagtgagatt
    16621 gatccgtcgt cctgcggcgg ggcctggcga ggcaaatcca aatcggcagc ggtgatatgc
    16681 gtgccttccg ccatgaccac ggcacgtccg atcctgttgg ataactcccg cacattgccc
    16741 ggccaggaat aggcctgcat cgcctccagc gcctcacgag taaacccgta cagaggttta
    16801 ttgtagtgag cagcggcctg ccgcagaaac gccatggcga tcagcgacac atcctcgcct
    16861 cgttcccgta acggaggcag gttgatgtgc accacgccca gccgataata caggtcatcc
    16921 cgaaaggtcc ccttctcgat cgcttccttc aaattcacgt ttgtggcggc aatgatccgc
    16981 acgttcatct caatcgcctg atgcccgccg actcgctcaa acgtgtgatc ctgnnnnnnn
    17041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatccgat tgatactcgc actcatgccc
    17161 aacatgccgc tgaattcttc gggcccgcca accaccatgg ccccgcgcgc ttcctgttcc
    17221 agatcggcga cccgcgcgac tcgttctata ctctgcagta attgcactgg atcaagggac
    17281 ttggcgagga catcacaggc cccgcactga accgcgcgca ccgccacact tcgctcaggt
    17341 ctcccggtgt acgcgatgac tttcccggaa taccccgtct gacgcaattt ctggagcaca
    17401 ttcaaccctt cttcggcaga cggctgcgat ggcgaatcga tatcgagcag catgaccggc
    17461 gaagcctgtt catgaatggg caacagcgtg tccgtcgtcc acattgccgt cgagacttcg
    17521 aactcgtcct gaatcaatgc ctgcgccgca gacaccgtcg actgatcagg cccgacaatt
    17581 actacactat gcttcgccac gattcggctc ctttcggctg gtaagaccac gggcgccatt
    17641 caggcaaacc atcgtctcac gaacggcaag atcgagataa ggatgcggct caagtccgca
    17701 tgacagctcg ttatgagacc gtcgacggag ggtcgagaga gtcgcacata aacacatctc
    17761 cgccgaatgc tttcgccagc gctggcaagg tgccattcgc gtcctgagcg gtgcgatcaa
    17821 gcatcgcgat gattggcact tgcgggcagg tacgtttcaa acggggaacc gctgtctgca
    17881 gcggcgtgga gcatgatgac aacccaacga caatcacatc gggacgcagt tcctgaaggt
    17941 gatgttccaa atgaggcaca gcggacgtca tggtggcttg gtatccatgg agcgcaaacc
    18001 agtcggcata gcgatacccg ctggtgacat cttcataaat caccgcgacc gtcggccgtt
    18061 ttccggaacg cgccccattg ccgcctgttg atgattgccc cataagattc cctccccaga
    18121 tgaatggcat caggttgtgg tgtgcatctt tgcgagttgg accaaccggt gtttcatttg
    18181 cggcccccac caggtcaggt cctgtaacat cgcggcctct cgtgggctca gtcttgtcag
    18241 gagatccgac cacccttgac gaaacgaccc cgttcgtcga tcaacagatg atcgctcggc
    18301 agccgcatgg ccttgcgctg cgtcaaaaat ggccactaat gtcgcggtat tagagatcca
    18361 ggccctctgc atttggaaga gcataggccg ggagggtcga ggaggctatc aacagaggcg
    18421 ctattcgtgt gtcacaataa aacagtgccg attcgtcatc gttctatcga cacatttagg
    18481 cacatcagcg ccgtcagaca acgaggccat gctggagggc ataccgggtc agctcggcat
    18541 tgttcttcaa gttcattttg tccagaattc tcgcccgata tgtgctgatc gtcgtcacgc
    18601 tcaggtggat actctccgcg atatcgctga cggtttgtcc tgaggcgatg aggcacagca
    18661 cttcatactc ccgatccgac aatcgctcgt gcggnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnnnnnnn nnnnccttcg aatccttcct ccagaatgtc cttcactcca cgccggaagg
    18841 agggatgatc atcgacgacc agaatacgta cggttgctgt gtttcgcatc gttccctcca
    18901 tcatgcgcta tccctttcct tctcgaggca gttgcaatgt gatagtcgtc ccgttgcctt
    18961 ccaaggtctc tatttgcaca tctccaccaa gcagcccggc tcgctcccgc attccgagca
    19021 gaccgaatga tcgggcatcg gcaagtcgat cttgaggaat tcctcgcccg ttgtcgcgga
    19081 cctgcagcaa taaccccacg ttctgctctt ccaatcgcac atgcacgtct gtcgcctgtg
    19141 catggcgggt gacattggtc agcgcctcct gacaaatgcg aaagaccgca gtggcctgct
    19201 ccgggtccac acgcaaatcc tcatggctga ccacgcaatg acaagtaatg ccggtacgac
    19261 gttgaaaatc ccggcattgc cattcaatcg ccgcgaccag ccccagatcg tccaacacgc
    19321 cggggcgcaa ttccgcgacg atccgctgca cagaggtaat ggtgctgtcg acttgctcct
    19381 tcatgccgcg aatcttctca tcgaccttgg cccgatcgcc agaaacgagc cgctcgccca
    19441 acagaccgcc caggcgggat aaatcgatct tcaggcacgt caggccgacg cccagctcgt
    19501 cgtgcaattc ccgcgcgatg cgtccgcgct cctcctcgcg tatcccctcc attcggcccg
    19561 agagttttcg cagccgacga agagaatcct gcaactgcgc ttcggcgcgc tttcgatccg
    19621 tcgtgtcctt gaagacgacg accgcgccga caagttcccc gcgctcagaa atgggcgtac
    19681 tcacatattc caccggaaaa ctcgtcccgt ccttgcgcca gaatgcgctg tccacctgct
    19741 cgcgcacctc gccgtcccga atcgtctcca cgatgggaca actcagcgcg ccgaacggtc
    19801 gttcagcgag atcgggagag aacaccaagg cggataacga ccggccgatc atggcgtctg
    19861 cttcccaccc caacatcttg gccgccgccg ggttgacgaa cgtcaaacgc ccctcgcgat
    19921 ccaccccgta gatgccttct cccgccgaat tcagaacgac ctgattgtga taatgcaggc
    19981 gatccagctg ctcctctgcc gtccgccgtt cgctgatatc gcgcataatg atcgtgaaaa
    20041 acagatcgga ttttcccttc catgacgtca cgctcaactc caacggaaac tcgtcccctt
    20101 gcttccggcg gcccacgagt tc
//
LOCUS       DAOQDS010000067        20057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1036_length_20057_cov_20.632337, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000067 DAOQDS010000000
VERSION     DAOQDS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20057
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1036_length_20057_cov_20.632337"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1762
                     /locus_tag="PK589_14660"
     CDS             203..1762
                     /locus_tag="PK589_14660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNJ20901.1"
                     /translation="MTSFPWLSLIVFFPLIGAVICFLVKAESSRWVALGFTVGNFLIS
                     LPLWWLFDSSTANMQFVERASWISSPPVNYSLGMDGISFPLVLMTTFLMPFCVTVSWT
                     AIEKRVQLFMSMLLVMEAAMVGVFVALDFVLFYVFWEVMLIPMYLLIGVWGGPNRLYA
                     AIKFFLYTLAGSLLLLVAILALYFQGGHTFDILELSRGTYGASLQMWLFLAFFAAFAV
                     KVPMFPFHTWLPDAHVEAPTAGSVILASVLLKMGTYGFLRFTLPMLPDATVAFTKPMI
                     ALSIVAIIYGAYMALAQSDIKKLIAYSSVSHMGFVTLGIFVLNIQGIEGAVMQMVNHG
                     ITTGGLFLCVGIIYERTHSRQILDNTGLAGPMPRYAMFLMIFALSSLGLPGTNSFVGE
                     FLVLAGTFVWSQFATAFAALGVILAAAYILWLMQRVVFGTPAPAHRAHLLDLNARETA
                     TLVPLIALVFVLGIFPNPLLTRMHASVTNLLNAQKAPAYATAPQQPALPQPAATMAAM
                     SSSSSEQAAAR"
     gene            1759..3234
                     /locus_tag="PK589_14665"
     CDS             1759..3234
                     /locus_tag="PK589_14665"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNJ20902.1"
                     /translation="MTFPLQDLLAILPELIVIGTACLILALDPILGADKKDVLAWLTL
                     GALAMCMGLTSSHLTGQTYAFSNLVVIDSYGAFWKLLLYIVTGLTVLLSLAYLKAERL
                     SIGEYYGFILLALVGMMVMVSGADLLTIYLGTELMSLSLYVMAGLKRTEARSLEASAK
                     YFVLGAFSSGVLLYGISLLFGLAGSTRLSVIAEAITTHGTTNPMLSLALVLLAVGFGF
                     KLAVVPFHMWTPDVYQGAPTSVTAFMAVASKAASFGAFLRVFVEGLGGASADWSVLFT
                     IICLATLALGNLVAIVQTSIKRMLAYSSIAHAGYALIGVVVAARHSGEGTSSGFASVL
                     LYIAVYSFMTLGAFALVGMLRKEGQESENIEDYAGLAKREPLAAFFMLVFLVSLAGIP
                     PTAGFIGKFYIFMAAVNGGMTWLAVVAVIFAAISAFYYLRIVMVMYMREPEGAATAHS
                     RLETSPALSFVLACALAGVVFLGLFPNGLWSLATHAAPLLK"
     gene            3370..4221
                     /locus_tag="PK589_14670"
     CDS             3370..4221
                     /locus_tag="PK589_14670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNJ20903.1"
                     /translation="MTELTFLSNVFDTFRRRGCPSLAASLAFYSILSLFPMVFLLLYG
                     ISFIVSQDVIGYQFLLGFLKGFLPSMGERLAKDIRRVAEQEEVRWAVFLAFGWFSALV
                     FYELDYAMNTVFGTAAKRHPLISTLVAVALIWMLGFLTLISFVATQAIELLTTYAPRF
                     LGMNLVAIAAHDFLITYTLPFLLAFASVTCLYRFLPHQRPTWREATIGGAVFSLLWVS
                     AKVLFVTYLEDAAVYTQLYGSLLEVVLLLLWVYYSSALVLLGAVVTYECQCRRPALLD
                     ESATLPG"
     gene            complement(4297..5472)
                     /locus_tag="PK589_14675"
     CDS             complement(4297..5472)
                     /locus_tag="PK589_14675"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNJ20904.1"
                     /translation="MQGPDWKIGRMFGIPIHVHASWLFVFFFMTWSLATGYLPDTLPG
                     LSEPRYWAMGGVAALLLFASVLLHELGHSLVALRYRIPIGQITLFIFGGVAQMRKEPP
                     HPRAEFLIAIAGPLVSFILAGLSLVLVAVLELFPAESSLHGLMALGTLLGMVNTQLGL
                     FNLLPGFPLDGGRALRAGLWAWSKDYYRATSQAALVGLLFGVTFALFGALLLFGALSG
                     TASSALASSGGWIVLLGAFLFTAARGTRKQAAIRASLASVPVRELMVRNVVALSPDLS
                     LEEAVNQYFLPYGYGGFPVVHEDRLLGVVAVRDIQSVKNSLWAFRRVADIMQPASDDM
                     VVSPDLSAMQALEQMIAVGAERLVVVQDGQLLGLVTRASIGHFIEQHHPSGLGKSSS"
     gene            5544..6266
                     /locus_tag="PK589_14680"
     CDS             5544..6266
                     /locus_tag="PK589_14680"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNJ20905.1"
                     /translation="MRLKDCWIGIAAICGLVFITEARPSSAQVIKSGPASCPAVALTY
                     DLCPVRTASGFDRELIDYLIEHKVPTTFFMSGRWMIKHDAEVKQLLAVPFFEIGTHGD
                     VHAHLPMHDVEEQQQEIMGPVRLLKTKYGRPATLFRPPYGEYNDETVDAVKALGLRFI
                     LWNIESGDPDPALSADAILTRVQKRLKPGSVIVLHANGKGKHTREVTEALAGQLLPDK
                     GLAPMTISDLLRCNQSTVAPKP"
     gene            6233..>7173
                     /locus_tag="PK589_14685"
     CDS             6233..>7173
                     /locus_tag="PK589_14685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ20906.1"
                     /translation="MQPIDSRAQALIRDMREEDRDAVIAILTNSDPWKRLGFAASDWL
                     RIFAPLPAGRDTFILEVEGAVLGIAIVRRKFLFGDYLELLGIAPSATGRGFGSSLLSH
                     IESLTFARAQNLFACVSDFNEAARAFYRKQGYKEIGPMPNFLIPGYAEILLRKTAGPA
                     RKWRHTIETADGDHVPQTVNGVPLMKVTKLLHTRMRVSDIDQTIRFYQNVLGLEVMDR
                     KVSPRGSHLAFLKVPNSDELIELCSFPASGPVTVQEDLVHLAFEVENLDETIRSLESK
                     GVRITDGPTPTSSGSRFLFIDAPDGYEVELIERPPGTA"
     assembly_gap    7174..7273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7274..7459
                     /locus_tag="PK589_14690"
     CDS             <7274..7459
                     /locus_tag="PK589_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20907.1"
                     /translation="STHRTVMKWSSSSARRVPPSCKRRHHTTFPPSVSPQAYANPLTN
                     LNPSTYCSLALRGTIVE"
     gene            7477..8346
                     /gene="dapF"
                     /locus_tag="PK589_14695"
     CDS             7477..8346
                     /gene="dapF"
                     /locus_tag="PK589_14695"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNJ20908.1"
                     /translation="MKNGFFRGHGLGNDYVVMDPKELSFNLTPGKIRGICDRHWGLGS
                     DGILALVPSKKADFGLRIYNPDGSEAEKSGNGLRIFARYLHATGKTKKRAFTVETKGG
                     LVSITLHVDRHGDAAAATVEMGRATFQPSALPCTLSVEELIQAPIEAAGRSLQFTGVS
                     VGNPHCVVFKEAGQSWTREELLTLGPALENHPLFPKRTNVQLAVPTGPKEIFILIWER
                     GAGETQASGSSSCAAASAAVKLGLVKGPVKVIMPGGTLNIEVAKDFSLTMKGPVAEVA
                     RGSLSPSFVRTLR"
     gene            8468..>9722
                     /gene="uvrC"
                     /locus_tag="PK589_14700"
     CDS             8468..>9722
                     /gene="uvrC"
                     /locus_tag="PK589_14700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNJ20909.1"
                     /translation="MAGSCETLQSKLDHLPEQPGCYLFRNAKREILYVGKAAVLADRV
                     RSYFQKGSDQTPKTSLLVSEVADLETIVTRSELEALILESNLIKRHRPRFNIVLRDDK
                     QYPYLRLPIKESFPRLSIVRRVQKDGALYYGPYTPAGALRETLKVIRKAFPLATCDID
                     IDGRADRACIEFEIKRCMAPCTGNQSREDYHVIVKQVRQFLEGRDRELLDSLRQAMES
                     AAEREEFEEAARLRDRLFSVERTLEKQRITQVTTTDQDVIGIARQGTAADLQLLFVRG
                     GLLIGRKDFFWPQSADSPDEELVRSAIEQFYNKEGQPPKELLVPTDLVDAPLIEQWLS
                     DKRGDTVRLLTPERGAKHQLVLLAEENAAAAIADHLRNEALDRQATAELKRLLRLDTV
                     PRRIEGFDISNIMGNQSVASLVVWED"
     assembly_gap    9723..9822
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9823..10437
                     /locus_tag="PK589_14705"
     CDS             <9823..10437
                     /locus_tag="PK589_14705"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNJ20910.1"
                     /translation="ISNIMGNQSVASLVVWEDGQAKKSDYRKFRIQTVEGANDFASMQ
                     EAVMRRYGATEDLARPDLILIDGGLGQLSAALEGLKQVGQEQIPIMGLAKARGEKDER
                     IFLPGRKNPIVLRPTSPATHLVQRIRDEAHRFAITYHRNLRGKALLSSELDQIPGIGK
                     IRRSRLLKQFGSLPQIASATDEQLKSAGLDATTVAALRKTLIPS"
     gene            10685..11317
                     /locus_tag="PK589_14710"
     CDS             10685..11317
                     /locus_tag="PK589_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20911.1"
                     /translation="MRMLRIAGTLLFASLAACSTPPEIKQALAAKDQAYAENEQLMQQ
                     YRELVSNVTTRHLKWYRYVQTRLKLDLALQWATTNPRVADVPDAALAEDDAAALGPDV
                     LTLVNAMRLKSLPERKGPNGQPLFAAGSGDMSTLIQQLPDLIARVEQQVAAGAKIPQT
                     VDLTAFDRYRNNVEALRRINGLIKQYLDIDVTASRSDVQSLADALRTVHR"
     gene            11353..12063
                     /locus_tag="PK589_14715"
     CDS             11353..12063
                     /locus_tag="PK589_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20912.1"
                     /translation="MVRRGMTLLSLAMLLLMATGCHSIHSRSVRDLIQMEGTKIDAAQ
                     TNIDLFQKETEERITFLEQARSSLHESFKSLQIQEAKHRFALASFRNLTNKRGEAAYA
                     AAYLTSLIYMTDVQGLEKAVWDQFEEDFCALRDTANALNDSWKHTATLHAEVQRFAKQ
                     SALASVDPEFVAAILDQAPGRSEQIMSVLNHSRTVNDALEEVVGARILRVGGLDRAQR
                     FTADLVELLDRLKKDDGQ"
     gene            12075..12596
                     /locus_tag="PK589_14720"
     CDS             12075..12596
                     /locus_tag="PK589_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20913.1"
                     /translation="MNRSIDGVITFLRDNDNVIKQLMETAESATNDIEQLATSVDHAR
                     EEAQVLVDQLGAVEFSVGRDDDTKVRQTALLEAVAALRKKFELYKNDPLRRGIYAAEI
                     SNLYLQLANTFNNETVAKIVPFEADEIETYKDLMSKAVLDAASRKKRAAVIKAGVQIS
                     RLALGVAGKLALV"
     gene            12601..13386
                     /locus_tag="PK589_14725"
     CDS             12601..13386
                     /locus_tag="PK589_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20914.1"
                     /translation="MATRFIMLNSAAIMLLLLAAGCSLDAASRGPFGPEDPETAIPKK
                     PLPAQVPTKHVLEFTADESPGHIKPKMAGSTNEAAVTRSPNPTASLSTSEIAGLRKTA
                     EADSRVRSLLGERWGFVDADPMPPTSKITFGCCRQQARFTKLTYFSYSHNVAVQVQLK
                     DGTVLQASRIDGYLPPEGPQDVQRGIELARADARLAGKVNGLQGHGLLMQPDRGFFKN
                     DPGYGHRVIWITFSQGVGGDPKYWAQVDLTDDRVLDAGDEPPR"
     gene            13405..14382
                     /locus_tag="PK589_14730"
     CDS             13405..14382
                     /locus_tag="PK589_14730"
                     /inference="COORDINATES: protein motif:HMM:NF013354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20915.1"
                     /translation="MRRFAGALLLCCAAAVPNLGFAESSSEHLDWGRWSFDYEVKDNT
                     GLAIRNVAYDGELVLGKASMPVLRVKYVKERVWWNPFTWFGSRADSGRCGPFQDRLRW
                     VDLVPIVNCGDNKVCVESATSHGVKWLELGIYARIGEYHIYQSWHFSEDGELRPVIQS
                     RGLSCNTDHIHHPYWRFDFDVNGNGMDQVFVRDNGGPDKGWGPGWTKYTNERNDVKHA
                     STQRTWFVRDQPTGHGVWLLPGDGYAPLKDDGERDSFADQDVAIRRAKPDEDVPWVFG
                     ARGQLGYDEDNQGVQEQDVVVWYVAHLPHMAALGPTKWLTLGPTLKIQR"
     gene            14631..15428
                     /locus_tag="PK589_14735"
     CDS             14631..15428
                     /locus_tag="PK589_14735"
                     /inference="COORDINATES: protein motif:HMM:NF015437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNJ20916.1"
                     /translation="MSQPAFSAKEFQRLGEIMYDARSVNRNDGVWQLVSAVQRVVPYE
                     FSGCGAVDLLHGIDPSLGHSTYPREFCQLYMGQGLAADPAVNRLLTSGQTVTSSADEP
                     VGNEPRDVTSLKLDFGIKTCLSAGVRGDHGACSYFAFSNFDAKQADKLRLLLDILTPH
                     FHLSYMRCKSAWTPDRPAQPALLLSKREEEILRWVAAGKTNWEISVILKVSLNTVKFH
                     LKNIFQKIGVENRWSAIAYWQNGEQHRIIPPSAPSDDRSPSDPNRPT"
     gene            15659..16930
                     /locus_tag="PK589_14740"
     CDS             15659..16930
                     /locus_tag="PK589_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20917.1"
                     /translation="MKQLAAHPAAMDATFTLLDLLQQRKTRRFGRGMTLPGGPLQYTS
                     HHDPVPLSREEERYLIYAAIGRSGRNLGDMQFVGRPGVSVGQGNALMNFNSRTVPSPC
                     SAQTTQLFYTNDDGVFFVADAAGPDHPWDLNVIQLQSSRLDIPREAPFMLPFNQWYTN
                     RPGTTLFMPVTNIASLYLNLLLMMFSEETGYFIVDTDNGNAACGLEAFRKSAGGHLHD
                     DMKARRMFSLRELDAAICETAIQEQGIICEHLSLMQQALGLGGGIQSVGSGRHLLGME
                     PHIYPGLGFHFVVPPGKPLRANPVGIPGVWEGPTPPFVPSMKDAVTNLVESKFGADGT
                     FRKPQEQPYVQRNTAQQVPQHSERAIEATIAFTEYVLATYGRFPAHADACKSIVACQT
                     HHLDEDFYATFYPDSALPDAHREHMHTWHSH"
     gene            16972..18297
                     /locus_tag="PK589_14745"
     CDS             16972..18297
                     /locus_tag="PK589_14745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNJ20918.1"
                     /translation="MTPQTSRRVVITGMGVISPLGSTVDLFWHLLSQGESAVRPITSF
                     DTSPFQACLGAEVRDFDPEDFLHRKQARRMGRATQFAVASAMMAARDSGIDLEQEDRG
                     SIGISIGTSIGGMKEAFEFHDAAKVTAYERVNPFTMGMTFPNAISSEVAIVLGLHGPC
                     ETYSIGCSSTANAIGRAYEWIKSGQASLVVAGGTEAPLHPSVYAAMNAGRALAPDERG
                     TIRNLPRPFDKTRCGMVLGEGAGCFILEEYEHARARGARMYAELEGWGFTCDAHSMVK
                     AATTGQEQQRAARLALSTAHWFPEEVDYVNACGLGTMELDALETHTVKQVLGTHAYRV
                     PISSFKAALGHAFAASGAFQLIGTAKAMEHQFIPPTLNLTNPDPTCDLDYVSGEGRAA
                     HIDRALINSFGFGGKNIVLALSRIDARVATTSPVGASAGYGLHHLAGVS"
     assembly_gap    18455..18554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18811..>20057
                     /locus_tag="PK589_14750"
     CDS             18811..>20057
                     /locus_tag="PK589_14750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNJ20919.1"
                     /translation="MHHLRSFQLRDMTACGAALRQSGAGAQSFDEVADRLVRHLYTSL
                     TMPQTGEPACALIRLFKTTSYEHLAPDLKVLADQRLGGTAGAPSMTCLTLSASAGMVP
                     GWNDHTLSSRFRVIPLRTSEELDKLPMFSQLFRQLGVGLPDLARPNTSLLVDPGEHAF
                     NVFHVPEAAGSPYVPTQEGFVEKYGVRSVLGFGAPLPDGQLFSIILFSQDVIPESTAQ
                     LFKPLALCAQTALAPYAERSATNSVKSVEPAKNQNLDESSTIDHLKSRIAELERLLAV
                     QEQTVTTQAERMDLVVRGSEAGTWDWHIGTGQVTFNERWATMLGYQLHELEPNVGTWE
                     QLVHPDDLPAVMEKVSAHLRGDTPLYSTEHRLKTKSGTWCWVFDSGRVVERDASGNPV
                     RAAGIHLDISARKELEAAQALAQR"
BASE COUNT         4107 a         6054 c         5554 g         4042 t
ORIGIN      
        1 gagtcgcccg tgcggttgcc tggggcggct ggttgatccg tctgacacag agcggccaga
       61 cacaacatta cgccctgggc atgacattgg gcgcggtggt catcttgacg gtgtatttgc
      121 tgctgtagtt gaaagcgagt ggaatccctg acgcccggta cgctgtctta ggcttcgtgt
      181 ttcagttgtg gagtagattt tcgtgacgtc gtttccctgg ttgagcctca tcgtattttt
      241 cccgttgatc ggagcggtga tctgcttcct ggtcaaggct gagtcgtcgc gttgggtggc
      301 gctcggcttc accgtcggca atttcctcat ctccttgccg ctctggtggt tgttcgacag
      361 ctccaccgcc aacatgcagt tcgtggaacg ggcttcctgg atcagttctc cgccggtgaa
      421 ctatagcctg ggcatggatg gcatcagctt tccgctggtg ctgatgacca cattcctcat
      481 gccgttttgc gtcaccgtgt cctggacggc catcgagaaa cgcgtgcagc tgtttatgtc
      541 catgcttctc gtcatggaag cagccatggt gggcgtgttc gtggcgctcg acttcgtgct
      601 cttctacgtg ttctgggaag tcatgctgat tccgatgtat ctgctcatcg gcgtctgggg
      661 cggtccgaat cgcctgtacg cagccatcaa gttcttcctc tacacgttag ctgggagcct
      721 gctcctgctg gtcgccatcc tggcgttgta cttccagggc gggcatacgt tcgacatcct
      781 tgaactcagc cgcgggacct atggcgcgtc gctccagatg tggctgttcc tggccttctt
      841 tgccgccttc gcggtcaagg ttccgatgtt tccgttccat acctggctgc cggacgcgca
      901 cgtagaggct cccacggcgg gcagcgtcat tctggcgagc gtgttgctga aaatgggcac
      961 ctacggattc ctccgcttca cattgccgat gcttcccgac gccacggtgg cctttaccaa
     1021 accaatgatc gccctgtcga tcgtggccat catctatggg gcctacatgg cgctggcgca
     1081 gtcggatatc aagaagttga tcgcgtattc cagcgtcagt cacatgggct tcgtaacgct
     1141 gggcatcttc gtgctgaaca tccagggcat tgaaggggcg gtcatgcaga tggtcaacca
     1201 cggcatcacc accgggggct tgttcctgtg cgtgggaatc atctacgagc ggactcatag
     1261 ccgccagatt ctcgacaaca cgggactggc cggtcccatg ccgcgttacg ccatgttcct
     1321 gatgattttt gccctgtcct ccttgggact tccgggcacc aacagtttcg tcggcgaatt
     1381 cctcgttttg gccggcactt tcgtctggag tcagtttgcg acggccttcg ccgcattggg
     1441 cgtgatcctg gccgctgcct atatcctctg gctcatgcaa cgggttgtgt tcggtacgcc
     1501 cgcaccggcg catcgcgcac atctcctgga cctgaatgcg agagagaccg ccacgcttgt
     1561 tcccttaatt gcgctggtct tcgtgctggg catcttcccg aacccgctgt tgacccgtat
     1621 gcatgccagc gtgacgaatc tcttgaacgc acaaaaggct cccgcatatg cgactgcgcc
     1681 gcagcaacct gcgctcccgc aaccggcagc aacgatggcg gccatgtctt cctcatcttc
     1741 tgaacaggca gcggcacgat gacttttcca ctccaagatc tcctggccat tcttcccgaa
     1801 ctgatcgtca tcggtacggc ctgcctcatt ctggcattgg acccgatcct gggagcagac
     1861 aaaaaggacg tcctcgcctg gctcacgttg ggcgctctgg cgatgtgcat gggcctgacc
     1921 tcgtcccacc tgaccggtca aacctatgcc ttcagtaatc tggtggtgat cgattcgtac
     1981 ggcgccttct ggaagctgct gttgtatatc gtcaccggcc tgaccgttct cctctcactg
     2041 gcctatctca aagccgagcg actcagcatc ggcgaatatt acggcttcat tctgctggcc
     2101 ttggtcggga tgatggtcat ggtgtccggc gccgacctgc tgaccattta tctcggcacc
     2161 gagttgatgt ccctttccct ctatgtgatg gccgggttga agagaacgga agcccgttcg
     2221 ctggaagcgt cagccaaata ctttgtgctg ggagcctttt cttccggcgt cttgctgtac
     2281 ggaatctctc tgctcttcgg cctggccggc agcaccaggt tgtcggtgat cgccgaggcg
     2341 atcaccacgc acggcactac caatccgatg ttgtcgctgg cactcgtact cctggccgtc
     2401 gggttcggat tcaagctagc ggtggtgccc tttcatatgt ggacgccgga cgtctaccaa
     2461 ggggctccga cctccgtcac cgcctttatg gccgtggcgt ccaaagccgc cagtttcggc
     2521 gcgttcctcc gggtgttcgt ggaaggcttg ggcggcgcga gtgccgattg gtcagtcctg
     2581 ttcaccatca tctgtctcgc caccttggcg ttaggaaatc tggtcgccat cgtacagacg
     2641 agcatcaaac gtatgctggc gtactccagt attgcccacg ccgggtacgc cctgatcggt
     2701 gtcgtcgtgg cagcccgtca cagcggcgaa gggacgtcca gcggtttcgc cagcgtctta
     2761 ctgtatatcg ccgtctattc cttcatgacc ctgggagcct ttgccttggt cggcatgttg
     2821 cgcaaagaag gacaggagag cgagaacatc gaggattatg ccggactggc caaacgcgaa
     2881 ccgctggctg cgtttttcat gctcgtgttt ctggtctcgc tggccggcat tcccccgacc
     2941 gctggattta tcggcaagtt ctatattttc atggccgccg tgaacggcgg catgacctgg
     3001 ctggcggtcg tcgcggttat cttcgccgct atttccgcct tctattactt gcggattgtg
     3061 atggtgatgt acatgcgcga gcccgaggga gccgccactg cgcactcgcg gctggaaacc
     3121 tctcccgcgc tgtcctttgt cctggcctgt gcccttgccg gcgtcgtctt tctcggtctc
     3181 ttccccaacg gcctgtggtc gctggccacc catgcggcac cgttgctgaa atagcctcca
     3241 ggcgcagggg tcacgccaca ttgaccggcg cctcgatttc ccgatgatgt gccaccgtgc
     3301 cttgtccggc tcctctcaac gatccgaagc attcaccgct ctgcactgag tctgaatcgc
     3361 gcggtctgca tgactgaact cacatttctc tccaacgtgt tcgacacctt tcgacgtcgc
     3421 ggctgtccca gcctggcagc ttcgttggcg ttctactcga tcctctccct cttcccgatg
     3481 gtcttcctgc tgctctacgg catcagtttc atcgtgagcc aggatgtgat cggctaccag
     3541 tttctgctcg ggtttctgaa aggattcctc cccagcatgg gcgaacggct ggccaaggat
     3601 atccggcggg tggctgagca ggaggaagtg cgatgggccg tctttctggc ctttggatgg
     3661 ttcagcgcac tcgtgttcta cgaattggac tatgccatga acaccgtgtt cggcacggca
     3721 gccaaacggc atccattaat ttccacgctg gtcgccgtag ccctgatctg gatgctgggg
     3781 tttctgacgc tcatatcctt cgtggccaca caggccatcg aactactcac tacctatgcg
     3841 ccgcgctttc tcggcatgaa tctggtagca atcgcggccc atgacttcct catcacctat
     3901 acgttgccat tcctgctggc cttcgcctcc gtcacttgtt tgtatcgctt tcttcctcac
     3961 cagcggccaa cctggcgcga agcgacgatc ggcggcgcgg tattcagtct cctctgggtc
     4021 tccgccaagg tcctcttcgt cacctatttg gaagatgcgg ccgtctacac gcaactgtat
     4081 ggttcactgc ttgaggtcgt cttactgttg ctgtgggtct actactcatc ggcactggtc
     4141 ctcctgggag ccgtggtgac ctacgaatgc caatgccgac gcccagccct cctcgacgag
     4201 tcggcgacgc ttcccggatg agttacgcgg gttgaggtct cacatgcctc agtggatggc
     4261 tatttatgcg gaatgtgagg gaaggaaacc gtccggttac gaagaagact tgccaagtcc
     4321 cgacggatgg tgttgctcga taaaatgtcc gatagatgcg cgcgtcacca gtcccagcaa
     4381 ttgaccatcc tggaccacaa ccagtcgttc ggcccccacc gctatcatct gctccaatgc
     4441 ctgcatcgct gacagatccg gcgagacgac catatcgtcg ctggcgggct gcatgatatc
     4501 ggccacgcgg cgaaaggccc agagagaatt tttgaccgat tgaatatcac gaaccgccac
     4561 gactcccaaa aggcggtcct catgcaccac cgggaaccct ccatagccgt aaggcaggaa
     4621 gtactggttc accgcttcct ccagcgagag atcaggagaa agcgccacca cattccggac
     4681 catgagttcc cgaaccggca cagacgcaag cgaagctcga atggctgctt gtttgcgggt
     4741 accgcgcgcc gccgtaaaaa gaaacgcgcc cagcagcaca atccacccgc cgctactcgc
     4801 caaggcgctg gaggccgtgc cggaaagagc cccgaacagc agtaacgccc cgaagagcgc
     4861 gaacgtgacc ccgaacagca gtccaaccag ggccgcttgg cttgtcgccc gataataatc
     4921 cttactccag gcccacaatc ccgcacgcaa tgcgcgccct ccgtccaagg ggaatccagg
     4981 caggaggtta aacagaccca actgagtgtt caccatgccg agcagggtgc ccaatgccat
     5041 caggccatgc agagacgact cggccggaaa caattccaag accgccacca gtacgaggga
     5101 aagtccggcc aggataaagc tcaccagcgg accggcgatc gcgatcagga attccgcccg
     5161 tggatggggg ggttcttttc gcatctgcgc caccccaccg aaaatgaaca gcgtaatctg
     5221 cccgatcggg atgcggtacc gcagcgccac caatgaatgc ccgagctcat gcagcaggac
     5281 ggaagcgaac aggaggagcg ccgccacgcc gcccatcgcc caatagcggg gctcggataa
     5341 gccgggaagc gtgtccggca aataacccgt cgccagcgac caggtcataa agaaaaacac
     5401 gaacagccag gaggcgtgca catggatggg aatgccgaac atgcggccga ttttccaatc
     5461 tggaccctgc atggtttcac cgtagcagcc acccggataa agcgtcaact atcgcaggtg
     5521 cgtgccatcg ggtatactgc ggcatgcgct tgaaagactg ctggatagga atcgccgcga
     5581 tctgcggctt ggtcttcatc acagaggccc ggccgtcgtc cgcacaggtc atcaaatccg
     5641 gtcctgcttc ctgccctgcc gtggcactga cctatgatct gtgccccgtt cgcaccgctt
     5701 cggggttcga tcgcgagttg atcgactacc tcattgagca caaggtgccg acgacctttt
     5761 tcatgtccgg tcggtggatg atcaaacacg atgccgaagt gaagcagctc ttagccgttc
     5821 cctttttcga gatcggcacc catggcgacg tgcatgcaca tctcccgatg catgatgtcg
     5881 aggagcagca acaggaaatc atgggaccgg tgcgtctcct caaaactaaa tacgggcggc
     5941 cggccacgct gttccggcca ccctatgggg aatacaatga cgagacagtc gacgcagtga
     6001 aagcactcgg cctgcgtttc attctgtgga atatcgaatc gggcgacccc gaccccgcgc
     6061 tgagcgccga tgcgatcctg acccgtgttc agaagcgact caaaccgggc agcgtcatcg
     6121 tcttgcacgc caatggaaaa gggaaacata ctcgtgaagt gaccgaggcc ctggccggac
     6181 agctcctgcc tgacaagggc ctcgctccaa tgaccatatc ggatcttctg cgatgcaacc
     6241 aatcgacagt cgcgcccaag ccctgattcg cgacatgcgc gaagaggatc gcgatgccgt
     6301 gatcgccatc ctgaccaact ccgacccctg gaagcgcctg gggtttgcgg cctcggactg
     6361 gctgcgcatc ttcgcgccgc tgccggccgg gcgcgacacc ttcattctgg aggtggaggg
     6421 agccgtgctg gggattgcga tcgtgcgcag aaagtttctg ttcggcgatt atctcgaatt
     6481 actcgggatc gcgccttcag cgaccggtcg tggattcggc agtagcctcc tctcccacat
     6541 cgaatcgctc acgttcgcgc gagcccagaa tcttttcgcc tgcgtatccg acttcaatga
     6601 ggccgcccgc gccttctacc gcaagcaggg ctacaaggaa atcggaccca tgccaaattt
     6661 tctgattccc ggatacgccg aaattctcct ccgcaagacg gccggcccgg cgaggaaatg
     6721 gcgtcacacc attgaaaccg cagatggaga ccatgttccg cagacagtga atggggtacc
     6781 actcatgaaa gtcaccaagc ttctccacac gcggatgcgc gtcagcgaca tagatcagac
     6841 catccgcttc tatcagaacg tcttaggctt ggaagtgatg gaccgtaaag tgtctccgcg
     6901 cggatcgcat ttggcgtttc taaaagttcc caacagcgac gaactcatcg aactgtgcag
     6961 ctttcccgca agcggaccgg tgactgtcca ggaagacctg gtccacctgg ccttcgaggt
     7021 cgaaaacctg gacgaaacca tccggagcct ggagtccaag ggcgtccgga ttaccgacgg
     7081 tcccacccct acgtcatccg gcagccgttt tctctttatc gacgcaccgg acggttatga
     7141 agtggagctc atcgagcgcc cgccgggtac cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnntcgacgc accggacggt tatgaagtgg agctcatcga gcgcccgccg
     7321 ggtaccgccc tcgtgtaagc gccggcatca caccacgttt cccccttcag tcagcccgca
     7381 ggcctatgcg aacccattga cgaatctgaa tccatccacg tattgttccc tcgctttacg
     7441 tggcaccatc gtcgaatgac aacaaggaga gtgcagatga agaacgggtt ttttcgcggg
     7501 cacggactcg ggaacgacta tgtagtaatg gatcccaagg aactcagctt caaccttacg
     7561 cccgggaaaa tccgcggcat ttgcgatcgc cattgggggc tcggcagcga cggcatcctc
     7621 gcgctggtcc cgtcgaagaa agccgatttc ggtctccgca tttataatcc ggatggtagc
     7681 gaagccgaaa aatccggcaa cggactccgt atcttcgccc gctatctgca cgccaccggc
     7741 aaaacgaaaa agcgcgcgtt cacggtcgaa acaaagggcg gtctggtttc gatcaccttg
     7801 catgtcgatc gccatggaga tgcggcggcg gccacggtag agatgggccg agccaccttc
     7861 caaccctcag cccttccctg cacgttgtcc gtcgaggaat tgatccaggc gcccatcgag
     7921 gctgccgggc ggtcattgca atttacgggt gtcagcgtcg gcaatcccca ctgcgtggtc
     7981 ttcaaggaag ccggtcaatc ctggacgcgt gaagaactcc tcacactggg accggccttg
     8041 gaaaaccatc ctctgtttcc aaaacgcacc aatgttcaac ttgccgtgcc caccgggcca
     8101 aaagaaatct tcatcctgat ttgggaacgc ggcgcagggg aaactcaggc ttccggctcc
     8161 tcgtcctgtg cggcagccag cgccgccgtc aagctcggac tggtgaaagg tccggtgaag
     8221 gtcatcatgc cggggggaac gctcaatatc gaggtcgcca aggatttcag tctgacgatg
     8281 aagggaccag tggcggaggt tgcgagagga agcctcagtc cgtccttcgt gcgaacgctt
     8341 cgctaaccag cgatcgacga tcggtcgtcc gcgcgagcac cacgttgcag gtcatttgct
     8401 acgaccgtta gaattactgg cgacgtggtt gcaatgttcc tcgctggtgg cggaaggctg
     8461 acttctcatg gctggatcgt gcgagaccct tcaatccaaa ctggaccatc tgccagaaca
     8521 gcccggctgt tatctcttcc ggaatgcgaa gagagagatt ctctatgtcg gcaaagccgc
     8581 cgtgctggca gatcgagtgc gctcctattt ccaaaaagga agcgaccaga cccccaagac
     8641 cagcctgctg gtcagcgaag tcgccgacct cgaaaccatc gtcacccgct ccgagctgga
     8701 agcgctgatc ctcgaaagca atctgatcaa gcgccaccgc ccgcgcttta atatcgtcct
     8761 gcgcgacgac aaacagtacc cctatctccg gttgccgatc aaagaatcct tcccgcgcct
     8821 ctccattgtc cggcgcgttc aaaaagacgg tgcgctgtat tacgggccct acacgccggc
     8881 cggcgcgctc cgagagacgc tcaaagtcat tcgcaaagcg tttccactgg ccacgtgcga
     8941 catcgacatc gacggacggg ccgaccgggc ctgcatcgaa tttgaaatta aacggtgtat
     9001 ggcaccgtgc accggcaatc agtcccgtga ggactatcat gtgatcgtca aacaggttcg
     9061 ccagtttctt gaaggccggg accgggaact cctcgacagt ctgcgtcagg ctatggagtc
     9121 ggcggcagaa cgggaggaat tcgaggaagc ggcccgcctg cgggaccgtc tctttagcgt
     9181 cgaacgcacg ctcgagaaac agcggattac ccaggtcacg accacggacc aggacgtaat
     9241 cgggatcgcg cggcagggca cggcagcaga tcttcagctc ctgttcgtac ggggagggct
     9301 cctgatcgga cgaaaggatt tcttctggcc gcaatccgcc gactcccctg atgaggaact
     9361 cgtccgttct gccatcgagc agttttataa caaggaaggg cagccgccga aggaactcct
     9421 ggtcccgacc gatctggtcg atgccccgct gatcgagcaa tggctcagtg acaaacgtgg
     9481 cgacacggtt cgactcctca ccccggaacg aggcgcaaag caccagctgg tcctgctcgc
     9541 agaggaaaat gccgccgccg ccattgccga ccatctgcgg aacgaagccc tcgatcgcca
     9601 ggccacagcc gaactgaaac gcctgcttcg cctcgacacc gtgccccgtc gcatcgaggg
     9661 gttcgacatt tccaacatca tgggcaatca atcggtcgcc tccctggtcg tgtgggagga
     9721 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatttccaa catcatgggc
     9841 aatcaatcgg tcgcctccct ggtcgtgtgg gaggacgggc aagcgaaaaa atcggactac
     9901 cgcaaattcc ggattcagac ggtggaaggc gccaacgact ttgccagtat gcaggaagcg
     9961 gtgatgcgac gatacggcgc gacggaagat ctcgcccggc ccgatctgat cctgatcgac
    10021 ggtggcctgg ggcagttgag cgctgcgctc gaaggattga aacaggtcgg ccaggaacag
    10081 atcccgatca tgggcttggc taaagcgcgt ggcgaaaaag atgaacgaat ctttctgccg
    10141 ggtcggaaaa atcccatcgt attgcggcca acctcacccg ctacgcatct ggtccagcgc
    10201 attcgtgatg aagcgcaccg ctttgctatc acctatcacc ggaacttacg gggcaaggcg
    10261 ctgctgtcat ccgagctgga ccaaattccc ggcatcggca agatccgtcg gtcacgcctt
    10321 ctgaaacaat tcggcagcct tccgcagatc gcctcggcca ccgatgaaca actcaagtcg
    10381 gccggcctcg atgcaaccac cgttgccgct ctacgcaaaa ccctcattcc ctcgtaaccc
    10441 gccaacacat acgtgcccgc ggaatgcagc tggcgctgta tgcggttcga tacacgcgta
    10501 tctgaatggg tacggtcgag agacgaggct cgggatgtgc cgattcgaca catcatgatt
    10561 ttccccttgc aggtcggtca tttcacgact actctggcgc gactccccac gatttatatc
    10621 gggtacgtag attgcctttc accagaatca tacgaatgga cgacgcttac caggaggcat
    10681 cccgatgagg atgttacgta tcgccggcac gctgctcttc gcctcactcg ccgcctgctc
    10741 caccccgcct gaaatcaaac aggcgctcgc ggccaaagac caagcctatg cggagaatga
    10801 gcagctcatg cagcagtacc gtgagctcgt gagtaatgtc accacccgtc atctgaagtg
    10861 gtatcgctat gtccaaaccc gtctcaaact cgatctggcc ctgcaatggg cgacgaccaa
    10921 cccccgcgtc gcggacgtgc cggacgccgc actggctgag gatgatgcag cggccctcgg
    10981 ccccgacgtg ctgacgttgg tcaatgcgat gcggttgaaa tcgctcccgg aacggaaagg
    11041 gccgaacggt caacccttgt ttgccgccgg atcaggagac atgagcaccc tcatccagca
    11101 actgccagac ttgatcgcac gggtcgaaca acaggtcgca gccggtgcaa agattcccca
    11161 gaccgtcgac ctcacggcat tcgaccgcta ccggaacaat gtcgaggcgc ttcgacgaat
    11221 caacggcttg atcaaacaat atctggacat cgacgtcacc gcgagccgat ccgacgtgca
    11281 atcgctggcc gacgccctgc gcacggtgca ccgatgagcg gcccctacac cgttccgcgg
    11341 aaggagcctt ccatggtccg acgaggcatg acgctgctgt ccctggccat gctgttgctc
    11401 atggctaccg gctgccattc cattcacagc cgttcagtcc gtgatctgat tcagatggaa
    11461 gggaccaaga tcgatgccgc ccaaaccaat atcgatctgt ttcaaaagga gaccgaggag
    11521 cggatcacat tcctcgagca ggcgcgaagt tcactgcatg agagtttcaa gtctctccaa
    11581 atacaggaag ccaagcaccg ctttgccctc gcgtccttcc ggaatctcac gaacaaaaga
    11641 ggcgaagcgg cgtatgcagc ggcctacctg acaagcctga tctacatgac cgacgtacaa
    11701 ggtctggaga aggcggtgtg ggatcaattt gaagaggact tctgcgccct gcgcgacacc
    11761 gccaacgcgc tcaacgattc ctggaaacac accgccacgc ttcatgccga agtccaacgc
    11821 tttgccaaac aatcggcgct agcctcggtc gatccggaat tcgtcgcggc gattctcgat
    11881 caagccccgg gacggtctga gcaaatcatg agcgtgctca accattcccg aacggtcaac
    11941 gatgcgctgg aagaagttgt cggcgcgcgc atcctccgcg tcggcggatt ggaccgggcg
    12001 cagagattca ccgctgatct ggtggaactc ctggatcgac tgaagaaaga cgacggacag
    12061 taacggaggg ctctatgaat cgcagcatcg acggggtgat taccttcctc cgggacaacg
    12121 acaacgtgat caagcagctg atggaaaccg ccgagtccgc caccaatgac atcgaacagc
    12181 tcgccaccag cgtggaccat gcccgcgagg aggcgcaggt gctggtggat caattgggcg
    12241 ccgtcgaatt cagtgtcggt cgcgatgatg atacgaaagt tcgccaaaca gcgctcctgg
    12301 aagcggtcgc cgccttgaga aaaaagtttg agctctacaa aaacgatccg ttacggcggg
    12361 gcatctacgc cgccgagatt tccaatctct atctgcagct ggcgaacacg tttaataatg
    12421 aaaccgtggc caaaatcgtt cccttcgaag ccgatgagat cgagacctac aaagacctga
    12481 tgagcaaagc ggtgttggat gccgcgtccc gtaaaaagcg cgcggcggtc atcaaagcgg
    12541 gagtgcagat ttcccggctg gcattgggcg tggcaggaaa actggcgctg gtataacgcg
    12601 atggcgacac gattcatcat gctgaacagc gccgccatca tgctcctgct actggcggcg
    12661 ggctgtagtt tggatgccgc gtcccgcggc cccttcggac cggaggatcc tgagacggcc
    12721 atcccgaaaa agccactgcc cgctcaggtt ccaacaaaac atgtcctgga gtttactgct
    12781 gacgaatctc caggccacat caaaccgaag atggccggca gcaccaatga ggccgccgta
    12841 actcgttccc cgaatcctac agcctctctt tccacatccg aaattgccgg gctgcgaaaa
    12901 actgcagagg ccgattctcg tgtgcggtct cttctggggg aacgctgggg attcgttgat
    12961 gcggacccga tgccgccgac cagcaaaatt accttcggct gttgccgcca acaggcccgt
    13021 ttcaccaagc tcacatactt cagctacagc cacaacgtcg cggtccaagt acagctcaaa
    13081 gacgggacgg tactccaggc gtcacgaatc gacgggtacc tgccgcctga agggccgcag
    13141 gatgttcaac gcgggatcga attggcgcgg gccgatgcca ggctcgccgg caaggtaaac
    13201 gggctacaag gccatgggct cctgatgcag ccggatcgcg ggtttttcaa aaatgatcca
    13261 gggtatggcc atcgggttat ctggattaca ttttcgcaag gagtgggcgg tgatccgaag
    13321 tattgggccc aggtggatct cacggatgac cgcgtattgg atgccggcga cgagcctcct
    13381 cgataaggca ggaaaggaga accaatgagg cgttttgcgg gtgccctcct gctatgttgc
    13441 gcagcggctg tgccgaacct ggggtttgcc gaatcctcgt cggaacatct cgactggggc
    13501 cgctggagct ttgattacga ggtcaaagac aataccggac tggccatccg gaacgtcgcc
    13561 tatgatgggg aactggttct gggcaaggcc agcatgccgg tcctgcgggt gaaatatgtc
    13621 aaagaacgcg tctggtggaa tccgttcacc tggttcggtt cccgtgcgga cagcggtcgt
    13681 tgcgggccat ttcaagatcg gttgcgctgg gtggatctgg tgccgatcgt gaactgcggc
    13741 gacaacaagg tgtgcgtcga aagcgccacc tcccatggtg tcaagtggct ggagcttggc
    13801 atttatgcgc gcatcgggga ataccacatt tatcaatcct ggcatttttc ggaagatggc
    13861 gaactccgcc cggtcatcca gagccgaggg ttatcctgca ataccgatca tatccaccac
    13921 ccctattggc ggttcgattt cgacgtgaac gggaatggaa tggatcaagt ctttgtacgg
    13981 gataacggag gaccagataa gggctggggg ccgggatgga ccaaatatac caatgaacga
    14041 aatgacgtca aacacgcctc gactcagcga acgtggtttg tgcgcgatca accgacgggg
    14101 catggcgtgt ggctccttcc cggggacggg tacgccccgc tcaaggacga tggcgaacgg
    14161 gactcgtttg ccgaccagga tgttgcgatt cgccgagcca agcccgatga agacgtgccc
    14221 tgggtctttg gcgcacgcgg ccaactgggg tatgacgagg acaaccaagg cgtccaggaa
    14281 caggacgtgg tggtctggta tgtggcacac cttccgcaca tggctgccct tggcccgacc
    14341 aagtggctga cactggggcc taccttaaaa attcaacgat aacccgttct tccctgcctc
    14401 tctccccgtc cgaccaaata atacgtgtat tcttccccgt tgcggttcat accctaggca
    14461 ctctatagta gggaccgttt accctagata ccgtcccttc ggtcgcagta caatcagccg
    14521 ttctcacgat gatacagctg cctcatcatg aaccacggcc gaatttctga acgcctctgg
    14581 ttgctgatca tccctatcgg gacagggtgc gttcgccgga aggaattggc atgagtcagc
    14641 cggcgttttc cgcaaaggaa tttcagcgac tcggcgagat tatgtatgac gcgcgctccg
    14701 tcaaccggaa tgacggggtg tggcagctgg tgtcggccgt gcagcgcgtc gtgccgtacg
    14761 agttttcagg atgcggcgcg gtcgatcttc tccacgggat cgatccttct ctcggacact
    14821 ccacgtaccc tcgagaattc tgccaactgt atatgggcca gggcctcgcg gcggatcctg
    14881 ctgtcaatcg tttgctgacc tcgggccaga ccgtcacctc cagcgccgat gaaccggtgg
    14941 gcaatgagcc cagagacgtc acgtcgctca agctcgattt cggcatcaaa acctgtcttt
    15001 cggcaggggt gcgcggggac catggagcct gctcctactt tgccttcagt aattttgacg
    15061 ccaaacaggc ggataagctg cgtctgctcc tcgacattct cactccccat ttccacttga
    15121 gttatatgcg ctgcaaatca gcctggacgc cggatcggcc agcccaacct gcattgctgc
    15181 tcagtaaacg ggaggaagaa attttacgat gggtggcggc aggcaaaacg aattgggaaa
    15241 tctccgtcat tctcaaggtc agtttgaata cggtcaaatt tcatctcaaa aatatcttcc
    15301 agaagatcgg cgtcgaaaac cggtggagcg ccattgccta ctggcaaaat ggtgaacagc
    15361 atcgcatcat tccaccatcg gcgcccagtg acgatcgttc tccgtccgat cccaaccgcc
    15421 cgacctaact cgcaccatgc atgtcgaccc gcgcgccgcc cagtccgtca ctacctgacc
    15481 gaccgggaac ggagccttcg cctaccccga tgggtaggtg acgccgagcc atacctctcg
    15541 taaagtctgc cccatacaca cgtcacgcgc aacgtgacgt gtcagcacaa cgtatcactg
    15601 ccactgacat gaccggccag ggccgaatgg ccccagggaa accggaagga gaggccctat
    15661 gaagcagctt gccgcacatc ctgcagcaat ggacgcaaca ttcactctgc tcgacctcct
    15721 acaacagcga aaaacgcgcc gcttcggccg cggaatgacc ctgcctggcg gtccgctgca
    15781 atacacgagt caccatgacc cggtcccact cagccgggaa gaggaacgct atctcattta
    15841 tgcggcgatc ggccgatcag gacgcaacct cggcgatatg caatttgttg gacgcccggg
    15901 ggtgagcgtc ggacaaggca atgcgctgat gaatttcaac agtcgcacag tccccagtcc
    15961 ctgctctgcg caaactacgc agctgttcta caccaacgat gacggggtct ttttcgtggc
    16021 cgacgccgca ggaccggatc acccctggga tctgaacgtc atacaactgc aatcatcccg
    16081 attggacatt ccccgggaag ccccgttcat gcttccgttc aatcaatggt atacgaatcg
    16141 gcccggcact accctcttca tgccggtgac caacatcgcc tccctgtatc tgaatctctt
    16201 gttgatgatg ttcagcgagg agaccggcta tttcatcgtc gatacggaca acggcaacgc
    16261 ggcctgcggg ctcgaggcct tccggaaaag cgcgggcggg catctgcatg acgacatgaa
    16321 agcccgccgc atgttcagcc tgcgtgaact cgatgcggcg atttgtgaaa cggccatcca
    16381 ggagcagggg atcatctgcg agcatctctc ccttatgcag caggcccttg ggctcggagg
    16441 cggaatccaa agcgtgggaa gcggtcggca cctcctgggc atggagcctc acatctaccc
    16501 cggactggga ttccacttcg tcgtcccccc gggcaagcca ttgcgcgcga atcccgtcgg
    16561 cattcctgga gtctgggaag gtcccacgcc gccgttcgtg ccatcgatga aggacgctgt
    16621 gaccaacctg gtcgagagca agttcggggc cgacggcacc ttcaggaaac cacaggaaca
    16681 accctacgta cagcgcaaca cggcacagca ggtcccgcag cattccgaac gcgccatcga
    16741 ggcgaccatc gcctttaccg agtatgtcct cgcgacgtac ggccggttcc cggcgcatgc
    16801 cgacgcctgc aagtcgatcg tggcctgtca gacgcatcat ctggacgaag acttttacgc
    16861 aaccttttat cctgactccg cgttgccgga cgcgcatcgc gaacacatgc acacgtggca
    16921 tagccattga cgacaaccat tttcacgctc ttacgagcac tggagggcat catgacacca
    16981 cagacctctc gtcgagtcgt catcaccgga atgggagtaa tttctccatt aggttcgaca
    17041 gttgatttgt tttggcactt attgagccag ggcgaaagcg cggtacggcc gattacgtca
    17101 ttcgacacct caccgtttca ggcttgcctt ggagcggaag tgcgggattt cgatcccgaa
    17161 gacttcctgc atcgcaaaca ggcgcggcgc atgggccggg cgacccaatt tgccgtggcc
    17221 tccgccatga tggccgcgcg cgattcgggc atcgatctgg agcaggaaga ccgcgggtcg
    17281 atcggcatca gcatcggcac ctccatcggc ggcatgaaag aagcgtttga atttcacgac
    17341 gcagctaaag tcaccgccta tgaacgggtc aatcccttca ccatgggcat gacctttccc
    17401 aatgccattt cgtcggaagt cgcgatcgtt ctcgggctgc acggtccttg cgaaacctat
    17461 tccatcggtt gttcctcgac ggcaaacgcg atcgggcgtg cctatgaatg gattaaatca
    17521 ggccaggcct cgctggttgt tgccggtgga acggaagccc ccctgcaccc gagtgtgtat
    17581 gcggccatga atgccggacg ggcgctggcg ccggatgaac gaggcacgat cagaaacctt
    17641 ccgcgaccgt tcgacaaaac gcgttgcggc atggtgctcg gcgaaggcgc cggctgtttc
    17701 attctggaag agtacgaaca tgcccgcgcg cggggagcca ggatgtatgc cgaactcgaa
    17761 ggctggggct ttacgtgcga tgcccattcg atggtgaaag cagccaccac gggtcaggaa
    17821 cagcagcgag cggccaggct ggcactgtca accgctcact ggttccctga ggaagtcgac
    17881 tatgtcaacg cgtgcggtct cggcaccatg gaattggatg cgctggaaac ccacaccgtg
    17941 aagcaggtct tgggcaccca cgcgtatcga gtgcccatca gttcatttaa agcggcactg
    18001 ggccatgcct ttgccgccag cggcgccttc caactcatcg ggacggccaa ggcgatggaa
    18061 catcaattca ttccgccgac attgaatctg acgaatccgg atcccacctg cgatctggat
    18121 tatgtgtccg gtgaagggcg ggccgcacat atcgaccgtg cgctgatcaa tagttttgga
    18181 ttcggtggca aaaacatcgt gctagcgctg tcccgcatag atgccagggt ggcaacgacc
    18241 agcccagtgg gtgcatcggc aggatatggc ctgcaccatt tggcaggagt gtcataggct
    18301 cacgacatga aggaccgcag actcatgacc acgggcatca caccagcagc ggtccatgcg
    18361 gcccggtctg tttcaggccg ggccgcacga tgccatgtca gtttcatcgt cggtcacagt
    18421 aagcagggtg gacaccgaag gccgagcgcc tcttnnnnnn nnnnnnnnnn nnnnnnnnnn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnncccctc atcgcacagg gcaaaaccaa taaggagatt gccgcggaat
    18601 tgggcctcag cgacaaaacc gtcaagaact acctggcgaa cgtgtactcc aaactccatc
    18661 tcaccaggag gtcccaagcc gccgcctttt acttgaaaac cattccctaa gggaacggcc
    18721 taacagcgac tggtgcgctg ctcaagttct tccgcgcgag cgccgacaga gtaccgacgg
    18781 actctctcgc ctccgcggga tcacctgagc atgcaccatt tacgatcctt ccagctccgt
    18841 gacatgacag cctgcggagc ggcactccgg caatcgggtg ccggagcgca gagcttcgac
    18901 gaagttgccg accgcctcgt tcgccatctt tacacgtcgt tgaccatgcc ccagaccgga
    18961 gagccggcct gcgccttgat ccgactgttt aaaaccacct cctacgaaca tctggcgcca
    19021 gacctcaaag ttctagccga tcaacggctt ggcggaacag ccggcgctcc atccatgacc
    19081 tgcctgaccc tctcggccag tgccggcatg gtcccaggct ggaatgacca cacattgtcg
    19141 agccgcttcc gggttattcc cctccgcaca tccgaggagc tggataagct tccgatgttc
    19201 agtcagctgt tccgccaatt gggtgtcgga ttacctgacc tcgcgagacc gaacacaagt
    19261 ctgcttgtag atcctggcga acatgccttc aacgtgttcc acgttcctga agcggcggga
    19321 agcccctatg taccgacgca agaggggttc gtcgagaaat atggagtccg atcggtattg
    19381 ggattcggcg cgccgctgcc cgatggacag ctcttctcga tcattctctt cagccaggat
    19441 gtgatccccg aaagcaccgc ccagctgttc aaacctctgg ctctttgcgc gcagacggcc
    19501 ctggcgcctt atgctgagag gtcggccacg aactccgtca aatccgtcga gcctgccaag
    19561 aaccagaacc tcgacgaatc ctcgaccatc gaccatttga agtcacgcat cgctgaactg
    19621 gagcgactcc ttgccgttca ggaacaaacg gtaaccaccc aagctgaacg gatggatctc
    19681 gtcgtccgtg ggtctgaggc aggcacctgg gactggcaca tcggcaccgg acaggtcacg
    19741 ttcaacgaac gctgggcaac catgctcggc taccaactgc atgaactcga accaaacgtt
    19801 ggcacctggg agcagctcgt ccatccggac gacctgcccg cggtgatgga gaaggtctct
    19861 gctcatcttc gcggcgacac acccttgtat tccactgaac atcgtctgaa gaccaagtcc
    19921 ggaacctggt gttgggtctt tgatagcggc cgtgtggttg agcgcgatgc ctcaggcaat
    19981 cccgtccgag ccgcgggcat ccatctcgat atttccgcgc gcaaggaatt ggaagcagcc
    20041 caggctctcg cccagcg
//
LOCUS       DAOQDS010000068        20048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1038_length_20048_cov_16.908568, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000068 DAOQDS010000000
VERSION     DAOQDS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20048
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1038_length_20048_cov_16.908568"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PK589_14755"
     CDS             complement(<1..1126)
                     /locus_tag="PK589_14755"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ20920.1"
                     /translation="MSRTWVTVGLLIMVSCLAQPSTAAWIDPLTPEERQGRNLYHYGK
                     AGIADPVAVNLQGVEGGVPPALFRCAGCHGHRGEGSQEGGLRVPPLTVSVLRRAREGT
                     SAGRPRAGYTDEALGKAITQGIDASGHALHPSMPRYQLTSDQVAKLIAYLTRLGEEAD
                     TDPGVSAEVVTIGAALPLTGPLAQIGQDVAATLRGALRRVNEQGGVYGRHIELIVEDS
                     RGDPAQTAQAVSRLIEIHQVFALVGSFEPAGGGRERFEQDQIPSIFPLTQSPQLSDPP
                     DPSIFYALPGFDLQFRSLMDFFASRTMPLTGTAQPRLALVHRNGDSNSEALRGALEQI
                     RHHALNLAMNYEYSTGRLAVLALVAQLEQQAIDAVVFLGDG"
     gene            complement(1159..1740)
                     /locus_tag="PK589_14760"
     CDS             complement(1159..1740)
                     /locus_tag="PK589_14760"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNJ20921.1"
                     /translation="MMLRFVPAAMLLWLCGAHAAPAWSGVTPPSIPDVEVIDQDGVPH
                     RFYGDMVKGKTVVINFIYTTCKTSCPAATGLLSGVYHRLQAAERPVTLLSVTVDPVHD
                     RPTRLKAYAAQFGAGKGWYFLTGAPADVARILASLQVSASNKAEHPAIVVIGNEAAGI
                     WTRLYGEASVPMVMDGLDATAGPPALTPSGISQ"
     gene            complement(1754..3718)
                     /locus_tag="PK589_14765"
     CDS             complement(1754..3718)
                     /locus_tag="PK589_14765"
                     /inference="COORDINATES: protein motif:HMM:NF014312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNJ20922.1"
                     /translation="MRNILVGARGSLPAFLLVAAPAIVGAGETAPSHPVQRFEQNGLA
                     IELTVVPQDGPSETGAELTAGQTATVKVALMDAKSGSPVVGIRPKGWMVGRPSEMAAD
                     EHSCVEKIRRLAGGRLGARAEVDLNRYVIVTMNHDKTVSVIDPQVEFGATKLESLVTL
                     PGNGADWVLSPDQSYLYVTIPGEDAVAVIETRTRKLIQTIALDKGSRPGRILWQPDSR
                     RLWVGLDGTGQVAVLDGEGHRRLKTVTVGRGLHTLAAIPDGRFIYVTNSQSDSVSVID
                     TQAMAPVVSVPVGKTPVAVTYSSAARRLYVGGVNGGDIVAIDPSTHRLVGRIPMAAGV
                     VALATEPQGRFVFAVNQVESSLSVIDTATSTVTATAHVVKDPDQIVFTQRYAYVRGIE
                     SEKFTLLDLREARNGKLVPLDVQAGRQAPSAAPQELGVAPMIVPTPEGNAVMIANAPD
                     ATLHYYQEGMMAPSGTFSNYRRMPRGVLVLDRSLREVAPGVYQATVTLPHPGRFDVPL
                     LLDQPRMSHCFQVTVQESPASTATAPGPRPVRIQARFESDLVSAHVPASLSFDLLDAT
                     TQEPIVGLHDVQVLIFEPPGTWQQRRWAQEERAGRYVITQSFPHAGEYRLLVQSPSRH
                     LRFSDAPPVLLTVGPPMSVSTGSSEQALKP"
     gene            complement(3727..9633)
                     /locus_tag="PK589_14770"
     CDS             complement(3727..9633)
                     /locus_tag="PK589_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20923.1"
                     /translation="MRDDEFVPASTQLSADMPGEDVQGAPSRPHVCTFSVVAKTAAIV
                     SLSLLWWGCAKPKMPESQQVVKPACERTITAEVVALDQVYYYNRFGAFNPAGIMFALR
                     RDVVHADEEDLLNGDLDNRSDEQEKAITLQHGPADLGLAGNVKLRSDKRPRPLVLRAN
                     EGDCLEVTFTNLLSPETNGQEVMLDPEKRLSRPDKRVTAVSDSDEPSTRHASMHVNGL
                     SLVDSIDSQGANVGRNGWHDQSGNKTDGRCSSNRSDFGKDLCALAAPGETRTYRWYAK
                     KEGTFFLYSMGAPAGGEGDGGQVGLGLFGAINVQPRGAKWYRSQVTEKDLKAASYKQE
                     TKFGQPVIDYEATYKNEPHAGDPILNMLKCDPRDNRQCELVHSDLNAVIDIDRENGCE
                     KMGEGNACGKPYREFTVIFHDEITAVQAFDEIREEGSPLSSLRDGMGINYGASGVGSM
                     VVANRNKLGPARDCVECKLEEFFLTSWVNGDPAMVVGYERGKGGSYKKDGNGRFVMQA
                     RYPDDPSNVHHSYLGDPVRFRNMHAGPKETHVFHLHAHQWVEDKHDPLSHYLDSQTIS
                     PGTTFSYEVHYGGSGNRNLGPGDSIFHCHLYPHFAQGMWELWRTHDVFENGGYDRRLK
                     DHDIAGGTPTPAIVPLPRTPLPPMPTPTFKGYPFYIAGEIGHRPPQPPLDLEGGDDAG
                     TLRRHVVAGGTVHDALDAQGPKEMKQAPPMNHDLFDKGRQQSAQIAARVGTQNTDKDL
                     VGLARELVTAKLDVKATAGTTDEKAAMKFHAGMSQVNGASAVRPGDPGWTYSPYNWEA
                     PGYVTCLSNGLCDDPHGTRVLFHVNGGGNIAEAKRGEGESGAPFSNPCPKQFIDEKGG
                     VHDVKTREYRAVYIQMNMTVNKSGWHDPQARFPILEQDVKETLDGTRPTEPLFFRAQS
                     GECVVFHATNLIPSNLNLDDFQVYTPTDTIGQHIHLVKFDVTSSDGSGNGWNYEDGTL
                     AADEVRERILAYNKTNPAQRLKPRTHRLFLRKGQLGATENGAMAGDPRGLCSDITGQA
                     ADDHDPVGHPWCGAQTTTQRWWADPVLNGKPGDKGTKDRTMRTVFTHDHFGPSSHQHH
                     GFYAALVVEPRNSKWYFPDGQLMGGTDSKGVPEAVNGRNDGGPTSYAANVVVRTQGKA
                     CITPDKDGILCSDALDSKGSIDAERTGREFNLAFADFAIVYDKDNQPVNPPSQIEGGL
                     RTPILPGRIPVPEGISTKDPGSQLINYRNEPIPLRIGTQDKDGHQFKQLPVDQEKGDL
                     AHAFSSTVHKDQKSVDERLFTEPSGKGRMPGDPATPLLRAYEGDHVQIRLIQGAQEEQ
                     HVFTMHGSKWLAQPDSPNTGYVNAQTIGISEHFEFNDRVVDFMPFGRSNGHKSLGGAG
                     EHTSIDHLYSSAATDNLWDGQWGLMRVYSRTEDEPGLVRLPGNAEKTHDVLASAFMQN
                     LDELLDRESLDVSDDDAGNEAGQNLAQSDALEAFCQKSGQAQLTRSYEVEAWLAKDLL
                     PSGKLVYNEHFGIADPNAIIFVDASEKAMLRSGSKRPEPLILRARAGDCIRVTLTNHL
                     PAAMQDGPDYPETWSYNMVPPIVPGFSFNQVRSSSRVGLHPQLVDYNLLKSDGAHVGL
                     NDDSTVAPGVSKTYLWYAGDIVPNKKGTRIVGIPHEFGAIGLQDMGDVIKHASHGAIG
                     ALLIEPQCSRWDTVPSTGNAEAVVTYWKPESASGRQGRHLKRQICPAEPKLDSGKLYR
                     FKEMVLLYQDNLSVQQYGQPVPNLRNGDDSEDSGQKGFNYRTEPLWARLGASAADEPE
                     TMSQFDWSNVLSSTVPHFRCEADFANKKYCDPETPLFTAKAGEPVRFRVVHPGGHPRQ
                     HAFTVFGHDWVPSPWIDASKAMGWNQDGLTRVGSAGGIGPGRDVNILTTAGGDCKVPG
                     DYLYRTQEGFMFGGGLWGIFRVEENPGLRWYQPAWAWAGNLFGYETNGVASADLCKVH
                     AANEAPIVQP"
     gene            complement(9674..11230)
                     /locus_tag="PK589_14775"
     CDS             complement(9674..11230)
                     /locus_tag="PK589_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20924.1"
                     /translation="MRVPSTVTVWMIGVLVLLGIAPPRHALSQAVIPFHIVGHIQRLT
                     LDSPADPLSGAKLTVNGVEVVLPKNLVIQLPAAYFTAQQLFDKAQGVSKKYGESGLAL
                     SDKFPPLAAFEADVSGNIVNGLYIAGLVTISQQSLNTGAGFIHHIDTATGMMCVGGSP
                     TAAACAGNDTRIRLNDPALDASDPFAGDGRYGKPNPAPPPVGLDDPNSRYPDPRFTVD
                     QGNPTVHALTGYPMCVPRATNDAQCPSQNRPAELTFVMDSVDLVPPVKFGNNAIKACP
                     SCDANKQAPVRVGDYITFSGTRARDPLAGDFLSVHTLVANVGIYTKPGGRAYVSLEES
                     LLGTRGPVVDCGAAAECQDRLKVEGFTTDPSRRVSVYAVDVVPGGVPKVRLLHSTEKD
                     QAVFGRFRYVPPLTAATLFDFNGNLKGATRELMVRIDDPAPLSDGSDVPSAPKAAHGL
                     TAGIYVAPVGEYIFPEPTGVQGGAQPALNFQCLAFLANGWALPDSGLPNIPRLTPWPG
                     VATPTFSCTQ"
     gene            complement(11730..12065)
                     /locus_tag="PK589_14780"
     CDS             complement(11730..12065)
                     /locus_tag="PK589_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20925.1"
                     /translation="MAFCAVILMSACAQSVHQTQRLAQQDFMLDPGYAGAQNYSYWPG
                     PGYGYGIGYGAGYWGGPLGYYGGPYGPLGFGWYGGYGGFGAARPPSSMQGPRPSPPPH
                     APPQFRKKY"
     gene            complement(12147..12419)
                     /locus_tag="PK589_14785"
     CDS             complement(12147..12419)
                     /locus_tag="PK589_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20926.1"
                     /translation="MIGSLLLTAGCAESWHQGQRHTVWSHQYNAGYGSAPVYGGAGYW
                     WNPFGYYGPGYGWYGGGSGGSHHSSSAPAPRPTPPDNAPPQFQKKY"
     gene            complement(12458..13096)
                     /locus_tag="PK589_14790"
     CDS             complement(12458..13096)
                     /locus_tag="PK589_14790"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ20927.1"
                     /translation="MAWIRYVRSRLSVVSLLAVLLLAPVASEAEWYAAAQLGVNFADP
                     IRNVRGSGSLAGLEAPNFNLKTSPAFGGKLGVFPNHGILGIELDVSHSTPHIKNLDDV
                     PGIHLSVTNIGPSVILRYPGMTWQPYIGVGPALLVAHLGRSTTTESDTQVSLGANVLA
                     GIRAFVTPKVAVFTEYKYTDSTFRFGGAFGPVGGFDGTYRAHQLFMGLSYHF"
     gene            complement(13299..13511)
                     /locus_tag="PK589_14795"
     CDS             complement(13299..13511)
                     /locus_tag="PK589_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20928.1"
                     /translation="MRKPGEPIYLRIHLIALALVLLATVALPRAAEFFLGPLSFGTRA
                     LAGIAIAVAGGIALYVLYNSSARNEP"
     gene            complement(13603..14808)
                     /locus_tag="PK589_14800"
     CDS             complement(13603..14808)
                     /locus_tag="PK589_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20929.1"
                     /translation="MTMPVQSPLSLAAFDLSPERGFLPATDPLDQLPEEPIINQIGTE
                     LPKFLTARQFRQFIQDRSDIMGPVADDWGLDVFRSAMRTLSFAGHAYVWEDPDHPAPS
                     LPASIAVPWYRIAQQLGRPPVLSYASYALHNWRRINPHQPIELGNIVLLQNFLGGLDE
                     EWFVLIHIDIEAKAGPGLQGLIDAQNAAQADDAESVLAGLRALAVAQEAMRETLLRMP
                     ERCDPYIYYSRVRPYIHGWKNSQALPQGLMYEGVTDYDERPQQFRGETGSQSSIVPAL
                     DAGLGVLHADDPLTQYLVEMRDYMPPRHRAFVAALEARTDGQQRPLLYGYARDRKMTH
                     PQLWKAFCTCVDLLAQFRDIHVGYAERYIFRQHQSHASNPTSVGTGGTPFMPYLNKHL
                     EETKQLIQD"
     gene            complement(15001..15585)
                     /gene="pth"
                     /locus_tag="PK589_14805"
     CDS             complement(15001..15585)
                     /gene="pth"
                     /locus_tag="PK589_14805"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNJ20930.1"
                     /translation="MALRLLVGLGNPGAEYAETRHNIGCWVIERAAARWSIRLTRKGA
                     AQRGSGRAGSKLVELAQTLDWMNLSGPPLKGLLRELGLTPESLVVVHDDLDLDPGRLR
                     IRQDGGSGGHNGLKSIIEALGTSKFVRLKVGVGRPAPRQDSADYVLEPVSSEERALYA
                     PCLDRAVDALELLLNRDVATAMNQFNVRDKLDDK"
     gene            complement(15585..16352)
                     /locus_tag="PK589_14810"
     CDS             complement(15585..16352)
                     /locus_tag="PK589_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNJ20931.1"
                     /translation="MKFELTVESREGGGKGPARQLRRAGRVPAVLYGQGECLLLSVKP
                     DELVKVLRSQAGSTALISLTINGAKSKPNRTALLREFQVDPITSSVLHADFFEVAMDK
                     PIRVKVPIHLTGGQPIGLKEGGVLHQALRELHVECLPSVLPDFIAVDASQLQINEGIH
                     LKDIPKTEGLRFLDDPDHMVVSVAAPMTDAKLESLLATGAAGAEGAKEPEVAAKGKAA
                     AEGAAGAEAGKAGDAKAGAAAPKAGAAPAKKEPEKKK"
     gene            complement(16390..17331)
                     /locus_tag="PK589_14815"
     CDS             complement(16390..17331)
                     /locus_tag="PK589_14815"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNJ20932.1"
                     /translation="MNRELKLFSGNANPALARAIGTYLDLPIGAATVSSFSDGEIRVR
                     IEENVRGADVFLIQSCCAPVNDSIMEMLIMIDALKRSSANRITAVIPYFGYARQDRKD
                     QPRVPISAKLVADLVTTAGADRVLTMDLHASQIQGFFNIPVDNLYALPVLLDYITKRR
                     IEDLVVVSPDAGGVERARAFAKRLQANLAIIDKRREGPNQAQVMNIIGDVQGKSALLL
                     DDMIDTAGTIVQGAQACLDKGAREVWAGCSHGVLSGPALERLQQSGLTDVLVTDSIPL
                     RGKEQKCPKLKVLSVAPLFGEAIRRIHEDESVSSLFV"
     assembly_gap    17788..17887
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17888..18684)
                     /gene="ispE"
                     /locus_tag="PK589_14820"
     CDS             complement(<17888..18684)
                     /gene="ispE"
                     /locus_tag="PK589_14820"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNJ20933.1"
                     /translation="MNSTSLLVLTPAKVNLILRVLERRPDGFHAIWSLMHTVGLNDEL
                     MLTMQPAAASRITLRCNQADLAVDQTNLVYRAAELVLARANRTMDLSISLTKRIPMGA
                     GLGGGSSDAAATILGLTRLLELNWSVEQMAEVGQQLGSDVPFFFVAPAACVTGRGEHV
                     RPVRVTGNRWIVLVSPGFPVPTKWAYQQLAATRAGVRPLSVAVQSLDQRSELDWSEIV
                     PLVENDFEEPVFAHHPVLAQIKSQLLSLGAEVALLSGSGATMFGLFR"
     gene            complement(18705..>20048)
                     /locus_tag="PK589_14825"
     CDS             complement(18705..>20048)
                     /locus_tag="PK589_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ20934.1"
                     /translation="LRDVLSIMLKRAGYAVTVASDGEEAIAQIHKEIFDLVITDLKMP
                     KMGGLDVLKAVKASSPDTVVLMITAFASADSAVEAMKHGAYDYLTKPFQVDEVQLIIR
                     NAIERRRLSTENMLLKRELASQSSFSQIVGQSEAMQKVYDVIKKVADSRSNVLIGGES
                     GTGKELVARAIHFNSARASMPFVTVNCSAVPETLLESELFGHMKGSFTGAVSNKAGLF
                     EVANGGTIFLDEIGDTTPAIQVKLLRVIQEREFRRVGGTQDVKVDVRIVAATNRDLEK
                     AVAEGAFREDLYYRLDVIPIRLPPLRMRTGDIPLLSQHFLEKFAKESGKPVPTMSQEA
                     MRVLLAHEWRGNVRELENVVERVVAFTTGSSVTDADIRGWLHKPVSTQQAVPSELPED
                     GLDLEGLINTIEKDLLLKALERSQWVKKRAARLLRLNTRSFRYRLEKYEIKGGRD"
BASE COUNT         4171 a         6171 c         5546 g         4060 t
ORIGIN      
        1 ctccatctcc cagaaatacc acggcatcga ttgcctgctg ttccaattgt gccactaggg
       61 ccagcactgc cagacgaccg gtcgagtact cgtaattcat cgcgagattc aacgcatgat
      121 gccgaatctg ttccagggca ccacgcaacg cctcactatt gctgtcccca ttcctgtgca
      181 cgagtgccag acgaggctgt gctgtccccg tgagaggcat cgtcctggag gcaaaaaaat
      241 ccatcaacga ccggaattga aggtcgaacc caggcaaggc atagaatatg gaagggtctg
      301 gtggatccga gagctgaggg gattgagtca gcggaaaaat cgaggggatc tgatcctgct
      361 cgaaacgttc acggccccct cctgccggct caaagctccc cacgagggcg aaaacctgat
      421 gaatctcgat caagcgggag acagcctgcg ccgtctgtgc aggatctccg cgagaatctt
      481 ctacaatgag ctcgatgtgc cggccgtaga cgccgccctg ctcgttcacg cgccgtagtg
      541 cgcccctcaa ggtcgcggcc acatcctgcc cgatctgtgc gagtggccca gtcaagggaa
      601 gcgccgctcc gattgtaaca acctcggcgc tcacgccggg atcggtatcg gcctcttccc
      661 cgagccgcgt cagatacgcg atcagcttcg ccacctgatc ggaggtcagc tgataccgcg
      721 gcatactcgg atgtaacgca tggccggaag cgtcgatgcc ttgagtaatg gccttcccca
      781 gcgcctcatc ggtgtatccg gcccgaggtc gtcccgcgct ggttccctcg cgcgcccgcc
      841 tgagcaccga tacggtgagt ggcggcactc gcaacccacc ttcctgtgat ccttccccac
      901 ggtgaccatg gcatcccgcg caccgaaaca gggccggcgg aacccctccc tcaaccccct
      961 gcaaatttac ggcaaccggg tccgcgattc cggcttttcc ataatgatac aggttgcggc
     1021 cttggcgctc ctccggcgtg agggggtcga tccaagctgc agtggagggc tgcgccaggc
     1081 aactcaccat gataagcagg ccgacggtga cccaagtcct gctcacgacg gggcatcgat
     1141 gtaccatggt gcgatgacct attgagagat gccggaaggc gtcaaggccg gcggacccgc
     1201 tgtggcgtca agcccgtcca tcaccatcgg aacagaggcc tcgccataca ggcgcgtcca
     1261 tatcccggcc gcctcattcc caatgaccac gatggctgga tgctctgcct tgtttgatgc
     1321 agagacctgc agggatgcca gaatgcgggc cacatcggca ggtgcgccgg tgagaaagta
     1381 ccatcccttc cctgcgccaa actgggccgc atacgcttta agccgtgtcg gccggtcgtg
     1441 aacggggtcg accgtcacag acaggagggt gacaggacgt tccgcggctt ggaggcggtg
     1501 gtacacccca ctgagcaagc cggtggctgc gggacaactc gttttacagg tcgtatagat
     1561 gaagttgatg accacggttt tgcccttcac catatcgccg taaaaacgat gtgggacacc
     1621 gtcctggtca atcacctcga catcggggat ggagggcggg gtgacaccgg accaggccgg
     1681 cgccgcatga gcgccacaca accacaagag catcgcggct ggaacgaagc ggagcatcat
     1741 agatcagacg ccctcatggc ttgagcgcct gctccgaaga gcctgtggac acagacatcg
     1801 gcggcccgac ggtgagcaga acaggcggag catctgagaa gcggaggtgg cgggatggcg
     1861 attgcaccaa cagccggtat tcccccgcat gcggaaaaga ctgggtgatc acataccggc
     1921 cggctcgttc ttcttgcgcc catcgccgct gttgccacgt gccgggcggc tcgaagatca
     1981 acacttgcac gtcatgaagc cccacgatcg gctcctgagt ggtggcatcc agcaagtcaa
     2041 acgacaagga agcgggaacg tgcgccgaca cgagatcact ttcgaaccgg gcttgtatgc
     2101 gtactggtcg agggcctgga gccgtcgctg tggatgccgg tgactcctga accgtcactt
     2161 gaaaacagtg ggacattcga ggctgatcaa gcagcagggg aacgtcgaat cggccagggt
     2221 gcggaagggt cacagtcgct tgatagacac cgggggccac ttctctcaag cttcgatcga
     2281 gcacgagaac gcctcgcggc atccgacgat aattcgagaa ggttcccgac ggagccatca
     2341 tgccttcctg ataataatgg agcgtcgcat ccggtgcatt ggcaatcatc acggcgttgc
     2401 cttcgggcgt cggaacaatc atgggcgcaa caccaagttc ctgtggcgcg gcactcggcg
     2461 cttgcctccc ggcttgcaca tcgagcggga ccagtttccc atttcgcgcc tcgcgcaggt
     2521 caaggagcgt aaatttttct gactcgatgc cacgcacata ggcgtatcgc tgtgtgaaca
     2581 cgatttgatc gggatctttg accacgtgtg cggtagccgt tacggtactc gtggcggtat
     2641 cgatgacaga caggctgctc tcaacctgat tgaccgcaaa gacaaaccgg ccctgaggtt
     2701 cggtggccaa agcaaccacg cccgcggcca tgggaatccg gcccacgagg cgatgcgtcg
     2761 acggatcgat cgctacaatg tctccgccgt tgacaccacc gacatacaaa cggcgggctg
     2821 cactgctgta cgtcaccgcg actggtgttt tgccaaccgg caccgacacc accggcgcca
     2881 tggcctgcgt gtcgatcaca gacacgctat cgctctggct gttcgtcaca tagatgaatc
     2941 gaccgtcggg aatcgcggcc aatgtatgca gacctcgtcc caccgtgacg gtcttgagcc
     3001 gtctgtgtcc ttctccatca agcaccgcca cctgacccgt tccgtccagc cccacccaga
     3061 gccggcggct gtcaggctgc cagagaattc gtcccgggcg ggatcccttg tcgagcgcaa
     3121 tagtctggat gagcttccgt gttcgagtct cgatcaccgc cacggcgtct tcgcccggaa
     3181 tcgtgacata gagataactc tgatccgggc tgaggaccca atccgctcca ttgccaggca
     3241 gcgtcacgag gctttccagc ttggtcgcgc caaactccac ctgcggatcg atcaccgata
     3301 ccgtcttgtc gtggttcatc gtgacaatca cgtatcgatt cagatcgact tcggcacggg
     3361 ctcccaatcg tcctccggcc agccggcgga tcttctcgac gcagctgtgt tcatccgccg
     3421 ccatttccga aggccgaccc accatccacc ccttcggcct tattcccacc actggagacc
     3481 cgcttttcgc atccatcagc gcgaccttca ccgtagctgt ctggccggca gtcagttctg
     3541 cccctgtctc ggaagggcca tcctgaggaa ccacggtcag ctcgatggca agcccattct
     3601 gctcaaaccg ttgcacagga tgggatggcg cagtctctcc ggccccgact atcgccggag
     3661 cggcaaccag caggaaagcc gggagacttc ccctagcccc gacaaggata ttgcgcatgg
     3721 cttgtctcat ggctgcacaa tcggcgcctc gttcgccgcg tgaactttgc acaggtcggc
     3781 cgaggccaca ccgttcgtct cgtacccaaa caggttgccg gcccaagccc aagccggttg
     3841 ataccagcgt aatccgggat tctcctcaac ccggaagatc ccccacaatc caccgccgaa
     3901 catgaacccc tcttgcgtgc gatagagata gtcgcccgga accttgcagt ccccgccggc
     3961 cgttgtgagg atattgacgt cgcggcccgg accaatcccg ccggctgatc cgacccgcgt
     4021 cagaccatcc tgattccagc ccatggcttt ggacgcatcg atccagggac tcggcaccca
     4081 gtcatggccg aacaccgtga acgcgtgctg tcgtggatgc cctcccggat gcaccacccg
     4141 gaaccgcacc ggctctcctg ccttcgcggt aaagagcggc gtttcgggat cacaatattt
     4201 cttgttcgca aaatccgcct cgcagcggaa gtgcggcacc gtcgagctca agacgtttga
     4261 ccaatcgaac tgactcatcg tctccggttc gtcggctgcg ctggccccta atcgcgccca
     4321 gagcggctcc gtgcggtaat tgaagccttt ctgccccgag tcttcggagt cgtcgccatt
     4381 ccgcaaattg ggaacaggct ggccatactg ctggacgctc aggttatcct gatagagcaa
     4441 caccatttct ttgaatcgat acaacttccc ggagtccagc ttgggctccg cggggcagat
     4501 ctgccgtttg agatgcctgc cctgccggcc cgaggccgat tccggcttcc aataggtcac
     4561 caccgcttcg gcgttgccgg tcgacggcac agtgtcccag cgtgaacatt gcggttcgat
     4621 gagtaatgcg ccgatcgcgc catgtgacgc atgcttgatg acatccccca tatcctgcaa
     4681 gccaatggcg ccaaactcat ggggaatgcc gacgatgcga gtccctttct tgttgggcac
     4741 aatgtcgcca gcgtaccaga ggtaggtctt gctgacacct ggcgcgacgg tggagtcgtc
     4801 attgagtccg acatgcgcgc cgtcactctt tagcaggtta tagtccacca actgtggatg
     4861 cagtccaacc cggctcgacg aacgcacctg attaaacgag aacccaggga cgatcggtgg
     4921 aaccatgttg taactccagg tctccggata gtcgggacca tcctgcatgg cggcagggag
     4981 atggttggtc agggtgacac ggatacaatc ccctgccctg gcacgaagaa tgagcggctc
     5041 cggccgcttg cttcctgacc gcagcatggc tttctcacta gcgtctacaa agataattgc
     5101 attggggtcc gcaataccga aatgctcgtt gtagaccaac ttcccactcg gcaacaggtc
     5161 cttggccagc caggcttcca cctcatagga acgagtcaac tgagcctgcc cgcttttttg
     5221 gcagaaggcc tccagcgcat cgctctgtgc caggttctga ccggcttcat ttcccgcgtc
     5281 atcatctgag acatccagtg attcgcgatc caacagctca tccaggtttt gcatgaaggc
     5341 tgacgccagg acatcgtgcg tcttctccgc attgcccggc aaacgaacca gccctggctc
     5401 gtcctcggta cgggaataga cacgcatcaa tccccactga ccatcccaca aattatccgt
     5461 ggcggctgag gagtagagat ggtcgatcga tgtgtgttcg cctgctccgc cgagcgattt
     5521 gtgtccgttg cttcgcccga acggcataaa gtccaccacg cgatcgttga attcaaaatg
     5581 ctcggaaatc ccgatcgtct gcgcatttac gtagccggtg tttggactat ccggctgggc
     5641 cagccatttg gagccatgca tggtgaacac atgctgttcc tcctgggcgc cctgaatcag
     5701 ccgaatctgg acatgatcgc cttcatacgc ccgcaggagc ggtgtcgccg gatcacctgg
     5761 catacgtcct tttccgctag gctcggtaaa cagacgctcg tcgacagatt tctggtcttt
     5821 gtgaaccgtt gaactgaacg catgcgccag atcgcctttc tcctgatcga cgggcaattg
     5881 tttgaactga tggccgtctt tgtcctgtgt gccgatgcgc agcggaatcg gttcgttccg
     5941 ataattgatc agctgggaac cggggtcttt cgtggagatg ccctccggca caggaatacg
     6001 gccggggaga ataggcgtcc gtaaaccacc ttcgatttga ctcggtgggt tcaccggttg
     6061 attgtctttg tcgtacacaa tcgcaaaatc ggcgaaggcc agattgaatt cccgcccggt
     6121 ccgttcggca tcgatgctgc ccttcgaatc gagcgcgtca ctgcaaagaa tcccgtcctt
     6181 gtccggtgta atgcaggcct tgccctgagt gcgtacgacc acgttcgcgg cataggaggt
     6241 cggtccaccg tcattgcgcc cgttcaccgc ttcaggcacc cccttggaat ccgtcccccc
     6301 catcagttgt ccatccggga aataccactt ggaatttctc ggctcgacca ccagcgcggc
     6361 ataaaacccg tggtgctgat gcgaactcgg gccaaaatgg tcgtgggtga acaccgtgcg
     6421 catcgtgcga tctttagtgc ccttgtcgcc aggctttccg ttcagcaccg gatccgccca
     6481 ccatcgttgc gtggtggttt gcgcgccaca ccagggatga ccgacgggat cgtgatcatc
     6541 tgccgcctgt cccgtgatat cgctacacaa cccgcgcgga tcccctgcca tggcgccgtt
     6601 ctcggtcgcg ccaagttgtc ccttccgaag gaagagacgg tgggtgcgag gcttgagccg
     6661 ttgcgcggga ttcgtcttat tgtaggccag aattcgctcc cgcacctcat cggcggctaa
     6721 cgtgccatct tcatagttcc atccattgcc tgatccatcc gatgaggtga cgtcgaattt
     6781 caccagatgg atgtgctgac cgatcgtatc ggtcggcgta tagacttgaa aatcatccag
     6841 gttgagattg ctcggaatca ggttggtcgc gtgaaagacc acgcactccc ctgactgcgc
     6901 ccggaagaac agcggctccg tggggcgagt cccgtccagc gtctctttca cgtcttgttc
     6961 caagatggga aaacgggctt gcggatcatg ccagcccgat ttgttgaccg tcatattcat
     7021 ctggatatag acggcgcgat actcgcgagt cttcacgtca tgcacgccac ccttctcatc
     7081 gatgaattgc ttgggacagg ggtttgaaaa cggtgcaccg gattccccct ctccgcgttt
     7141 ggcctccgca atgttcccac ctccgttcac atggaacaac acgcgcgtcc cgtgagggtc
     7201 gtcgcagagt ccgttagaga gacaggtgac atacccaggc gcttcccagt tatacggact
     7261 gtaggtccac cccggatctc ccgggcgaac ggcagaagca ccgtttacct gagacattcc
     7321 agcatgaaac ttcattgccg ccttttcgtc tgtcgtgccg gcggtcgcct tcacatccag
     7381 tttcgccgtg accaattcgc gcgccaaccc gactaaatct ttgtcggtat tctgggtacc
     7441 tacacgcgcg gcaatctgtg ccgactgctg ccgcccctta tcaaacaagt catggttcat
     7501 cggcggagcc tgcttcatct ccttcggccc ctgcgcatcc agcgcgtcgt gcaccgtccc
     7561 ccctgccacc acgtgtcggc gaagcgtgcc ggcatcgtcc ccgccttcca gatcgagcgg
     7621 cggctgcgga ggtcgatggc cgatttctcc ggcgatatag aacggatagc ccttgaacgt
     7681 cggagtcggc atagggggca gcggggtacg aggcaatggc acaatggccg gtgtgggagt
     7741 acccccggca atatcgtggt ccttcaaccg gcggtcgtag cctccatttt caaacacatc
     7801 gtgcgtccgc cagagttccc acatcccctg cgcaaaatgg ggatagaggt gacaatggaa
     7861 aatggaatcg cccggtccga gattgcggtt gccggagccg ccgtaatgca cctcgtacga
     7921 aaaggtcgtg cctggcgaga tcgtctgtga gtcgagataa tgggagaggg gatcgtgctt
     7981 atcctcgacc cattgatgcg catgcaaatg gaacacatgg gtctccttgg gacccgcatg
     8041 catgttccgg aatcggacgg gatcgccgag ataggaatga tgcacattgg agggatcgtc
     8101 gggataccgc gcctgcatta cgaatcgtcc gttcccgtcc ttcttgtaac tgccgccttt
     8161 cccccgctca tacccgacca ccatcgccgg atcgccattg acccaggagg tgaggaagaa
     8221 ttcttcgagt ttgcattcca cacaatctcg cgccgggccg agcttgttgc ggttggccac
     8281 aaccatcgac cctacgccag aggccccgta attgatgccc atgccgtcgc gcagtgaact
     8341 cagcggactt ccttcctccc ggatttcatc gaacgcctgg acggcggtga tctcatcatg
     8401 gaaaatcacg gtaaattccc ggtacggttt tccacaagca ttaccttcgc ccattttctc
     8461 gcagccgttt tcccggtcga tatcgatcac ggcattcagg tccgaatgga caagctcgca
     8521 ctgccggttg tctcgcggat cacacttgag catattcaaa atcgggtcac cggcatgtgg
     8581 ctcgttcttg tacgtagcct cataatcaat gaccggctga ccgaatttcg tctcttgctt
     8641 atagctggcg gctttcaagt ccttctcagt cacctgggag cgataccact ttgcgccacg
     8701 aggctgtaca ttgatcgccc cgaacaggcc taatccgact tgcccgccgt cgccctcgcc
     8761 gcccgcaggt gcccccatgg aatagagaaa aaacgtccct tcctttttgg cgtaccacct
     8821 gtacgttctg gtttcaccgg gtgccgccag cgcgcacaga tctttgccaa aatcacttcg
     8881 gttggatgaa caccggccat cggtcttgtt cccgctttgg tcatgccatc cattgcgacc
     8941 gacattcgcg ccctgagagt cgatggaatc caccaaactc aatccgttca catgcatgga
     9001 ggcatgtctg gtcgacggtt catcgctgtc actaacggcg gtcacgcgct tgtccggccg
     9061 ggagagccgc ttttccggat caagcatgac ttcctggccg ttcgtctcgg gagaaagcag
     9121 gttcgtgaag gtcacctcca gacagtctcc ttcattggcc cgaagcacca gcgggcgagg
     9181 ccgcttatcg gaacgcaatt tcacattccc agccaagccg agatcggcag gcccatgctg
     9241 aagggtaatt gccttctctt gttcgtccga ccgattgtcc aggtcgccat tgagaaggtc
     9301 ttcctcatcg gcatgcacaa catcccgtct cagcgcgaac atgatgccgg ccggattaaa
     9361 tgctccgaac cggttgtagt aatacacctg gtccaaggcc acgacttcag ccgtgatagt
     9421 cctctcacag gccggtttca ccacctgctg ggattccggc attttcggct tggcgcaccc
     9481 ccaccagagc agggacagac tgacaatcgc tgcggttttg gccactacgc tgaacgtaca
     9541 tacgtgcggt cttgatggag cgccttgaac atcttcgcca ggcatgtccg cactcaattg
     9601 ggtagacgct ggaacaaatt catcatctcg catggtcaag gtctccactg ggctcattcg
     9661 tgacaatcac accttattgc gtgcaggaaa acgtcggggt tgcgacgccc ggccaaggtg
     9721 tcaggcgagg aatattgggc aacccgctat cgggaagcgc ccatccgttg gcgagaaatg
     9781 ccagacattg gaaattcagg gctggttgag ccccgccttg cacaccagtt ggttccggga
     9841 aaatatattc gccgaccggt gcgacataga tgccggcggt cagaccatgg gcagccttgg
     9901 gtgcgctcgg gacatcgctg ccgtccgata gcggcgcggg gtcgtcaatg cgcaccatca
     9961 gctcgcgcgt ggctcccttc aaatttccgt tgaaatcaaa gagcgtagcg gcggtcaatg
    10021 gtggaacgta gcggaagcga ccaaagacgg cttgatcctt ttccgtgctg tgaagtaacc
    10081 ggaccttagg aactccgcct ggcaccacat ctaccgcata gacgctgaca cgccgacttg
    10141 gatcagtcgt gaagccttcg actttcaagc ggtcctgaca ttcagcagcc gcgccgcagt
    10201 ccacgaccgg ccccctcgtt cccagcagcg actcctccag gctcacgtac gcgcgcccac
    10261 cgggcttggt gtagataccg acgttcgcga ccagcgtatg cacggacaga aaatctcctg
    10321 ccagcggatc acgagccctc gtccccgaaa acgtgatgta gtcgccaacc cgcaccgggg
    10381 cttgcttatt cgcatcgcac gacgggcagg ccttgatcgc attgtttcca aacttgaccg
    10441 gcggcaccag atcgacggag tccatgacga aggtgagctc cgccggtcga ttctgactcg
    10501 ggcattgcgc gtcattggtg gcgcgcggca cgcacatggg atagccggtc agcgcatgca
    10561 ccgtgggatt gccttgatcg acggtaaaac gtggatcagg gtagcgagag ttcggatcat
    10621 ccagaccaac cggtgggggc gccggattcg gcttgccgta acgaccatct cctgcaaagg
    10681 gatccgaggc atccaaggcg ggatcattca ggcgaatgcg cgtatcgttg ccggcgcagg
    10741 ccgctgctgt cggactcccc cccacacaca tcatgccggt cgccgtatcg atgtgatgga
    10801 tgaaaccggc gccggtattc aacgattgtt gcgagatcgt gactagcccg gcgatataga
    10861 gcccattgac aatattcccg ctgacgtccg cttcaaaagc cgccaatggg ggaaacttgt
    10921 cgctcagtgc aagcccggat tcgccatatt tttttgagac gccctgggcc ttatcgaaga
    10981 gttgctgcgc agtgaagtac gcggcgggca actgaatcac gagattcttc ggcagcacca
    11041 cctccacgcc attcaccgtc agcttagcgc cggacagggg atccgccggg ctgtcgagcg
    11101 tgagccgctg gatgtgtccc acgatgtgaa agggaatcac cgcctgcgac aatgcatggc
    11161 gtggcggagc tatacccaat aagacaagta caccgatcat ccatacggtg acagtggacg
    11221 gtacacgcat cgagaatcct cctcctggaa acagacggcc acctaacttc aatgtattga
    11281 catgggatca ggcccggaag cagcaacccg cacgatggga gtacgtaggg gtatgactac
    11341 tgagtccgca tgaggccggg cacgagatac cagattgcac aatacttgcc aagctatttc
    11401 ctgcctgacc caacgaataa atacatacgg cgaggctcac ggatggaagc gtgaccatcc
    11461 tcatccccga gcaaatgcgg ttttcacgta tgcacaatgc tctgagtggc gctgtgtgcg
    11521 agcaatgctt tagaaagatg aatagcttca attgtggcgc agaggatgcg ccgtctgcag
    11581 aggatgggaa tggtttctta tcgatttgga tacaaggcag tattggaatg gatacagaat
    11641 ggattcaggc ggtgcgtatc acattgatac ggcacttctc acttcaggcg attcactaat
    11701 agaacagaac cgcagcgctg cacctccgct cagtatttct ttctgaactg cggaggcgcg
    11761 tgtggcggtg gcgaaggacg cggtccctgc atactcgatg gcggacgcgc cgcaccgaat
    11821 cctccatatc caccgtacca gccaaagcct aatggaccgt acggtccacc atagtatccc
    11881 agcggcccgc cccaataccc cgccccatat ccgatgccgt acccgtatcc gggtccaggc
    11941 caataggaat aattttgggc gcccgcatag ccggggtcga gcatgaagtc ttgctgggcc
    12001 agtcgttgcg tctggtgcac tgactgggca caggccgaca tcaagatcac ggcacagaat
    12061 gccacggcgc atgcgcggag gcgtgtacgt actgcttcat gtgcaattac cattcgtctg
    12121 tgcatggcga cgatgatctc tccgaatcaa tactttttct ggaactgggg cggcgcgtta
    12181 tccggcggag tcggccgagg tgccggcgca ctcgatgagt gatggctgcc gccagagccg
    12241 ccgccatacc atccatagcc ggggccataa tatccgaatg gattccacca gtatcctgct
    12301 cccccataga ccggtgcgct cccatacccg gcattgtact ggtggctcca tactgtgtgc
    12361 cgctgcccct gatgccagga ctccgcgcag cccgccgtca gcaggaggct accaatcatg
    12421 cagctccaga taattgctcg tatcatgccg cacctcgcta gaagtggtaa gacagcccca
    12481 tgaacagttg atgggcgcga taggtcccgt cgaatccgcc taccggcccg aacgctccgc
    12541 cgaagcgaaa cgtcgaatcg gtgtacttat attcggtgaa cactgcgacc ttcggtgtga
    12601 cgaaggcacg aataccggcc agcacatttg cgccgagcga cacttgcgtg tcgctttccg
    12661 tcgttgtaga tcggccaaga tgcgccacca acagggctgg gccaacgcca atatacggct
    12721 gccacgtcat gccgggatac cgcagaatga cgctcggtcc gatattggtc acagagaggt
    12781 gaatgcccgg cacatcgtcg agattcttga tatgcggagt gctatgggag acatctaact
    12841 caattcccag gatcccatga ttgggaaaca cgcccagctt tccgccgaag gcgggactgg
    12901 tcttcagatt gaaattaggc gcctccaacc cggcgagaga tccacttcct cgcacgttcc
    12961 ggataggatc ggcgaaattc acccccagct gagcggccgc ataccattct gcctcggatg
    13021 cgacgggagc aaggaggagt actgccaaca acgatacgac tgaaagcctg ctgcgcacat
    13081 aacgaatcca tgccatggtg aagtgctcca tgggcgaggc ttactaacat cataacaggc
    13141 gtccggcgta gaagactgga caactttgct gttctgactt ccgcgatgtc gaattacacg
    13201 tgccctcaat gggtcgctgc gcgagacgga tcaatgcagg atagttcgtg gtcgattcgg
    13261 tgacactgca gtgcaatcga agaagagcct cttaactgtt acggctcatt cctggcagaa
    13321 gaattataaa ggacgtacag cgcaatgcca cccgcgacag caattgcaat ccccgccaac
    13381 gcgcgggtgc caaagctcag cgggccaaga aaaaattccg ctgcgcgagg caatgccacc
    13441 gtggccagca gcaccaaggc cagagcgatc aggtgtatcc gaaggtaaat gggttcgccg
    13501 ggtttacgca tagatcaaca agtatcaaca gggcgcagtg tacgaactcc agccgcagta
    13561 ttccagtccg aaacgaggcg cggcgccatc gtccctgcgc ggttagtcct ggatgagttg
    13621 cttggtctcc tcaagatgct tgttcagata gggcataaat ggcgtcccac ctgtgcccac
    13681 ggaggtcgga ttactcgcat gcgactggtg ttgacgaaag atgtagcgct cggcataccc
    13741 gacatgaatg tcacgaaact gggccagcaa gtccacgcag gtgcagaatg ccttccataa
    13801 ttgcggatgc gtcatcttac ggtcgcgagc atacccgtaa agcagtggcc gctgctgccc
    13861 gtctgtacgc gcctccagtg cagccacaaa tgcgcggtgg cgtggcggca tatagtcccg
    13921 catctcgacc agatattgtg tcaggggatc atcggcatgg agcacaccaa gcccggcatc
    13981 caacgctggc acaatggaac tttgcgagcc ggtttctccg cggaattgct gcgggcgttc
    14041 atcatagtcc gtgacccctt cgtacatcag cccctgtggc agtgcctgac tatttttcca
    14101 tccatggata tagggtcgta cgcggctgta ataaatgtag ggatcgcacc gttccggcat
    14161 acgaaggagt gtctcccgca tcgcctcctg cgcaacggcg agcgcacgta acccggccag
    14221 caccgattcc gcgtcatccg cctgcgcggc attctgcgca tcaatcagcc cctgcaatcc
    14281 aggtccggcc ttcgcttcga tatcgatgtg gatcaacaca aaccattcct catcaagccc
    14341 gccgaggaaa ttctgaagga gcacaatgtt tccgagctca atcggttggt gtggattgat
    14401 ccggcgccag ttgtgcaacg cataggaggc gtacgacagg accggcggac ggcctaattg
    14461 ctgagcgatg cgataccacg gcacagcgat gctggccggc aaactgggag ctggatggtc
    14521 cgggtcctcc cacacgtagg catggccggc aaacgacagg gttcgcatgg ccgaacggaa
    14581 gacatcaagc ccccaatcgt ccgccacagg gcccatgata tctgaccgat cctgaataaa
    14641 ctgacggaac tgccgggcgg tgaggaactt cggtaactcc gtcccgatct ggttgatgat
    14701 tggctcttca ggaagctggt ccaaggggtc tgtggcagga agaaagccgc gctcagggga
    14761 gaggtcgaac gcggcgagtg agagaggcga ttgaactggc atcgtcatga ttctcccaag
    14821 gtaacgcgaa tagttgactc taccacaggg agagagatgg ctgctgccga cttgagaagc
    14881 cctgccatga atgcgtcatc gtaggaccac gttcttcgaa tgaacacgtc accgaataaa
    14941 agcgttggtt ttggaggaga tgacgaatag tccggacagg tgagagggat tgaacaatac
    15001 ttatttgtcg tccagcttgt cccgcacgtt gaattgattc atcgccgtgg cgacatcgcg
    15061 attcaagagc aactccagcg catcgacggc gcggtcaaga cagggggcgt acagggctcg
    15121 ttcctcagat gagactggct ccagtacata gtctgcggag tcttgccgcg gagccgggcg
    15181 ccccactccg accttcaagc ggacgaactt agatgtgccc agagcttcga tgatcgactt
    15241 gagaccattg tggccaccgc tgccgccgtc ttgcctgatg cgcaatcgac ccgggtccag
    15301 atccaggtcg tcatgcacga cgaccaagga ctcgggcgtc agacccagtt cacgaaggag
    15361 acctttgagg gggggaccgc taagattcat ccagtcgagc gtttgggcca gctcgacgag
    15421 tttggaaccg gcgcgacccg agccacgctg ggccgcgcct ttcctggtga gacgtatcga
    15481 ccatcgcgcc gccgcccgtt cgatcaccca gcagccgatg ttgtgccgcg tctcggcata
    15541 ctcggctccg ggatttccca atccaacgag aaggcgcaac gccactactt cttcttctct
    15601 ggttccttct tggctggagc cgcccctgcc ttgggcgcag ccgccccggc cttggcgtca
    15661 ccggccttac cggcttccgc accagccgcc ccttcagcag ccgctttccc cttggccgcc
    15721 acttccggtt ccttggcgcc ttcagccccg gctgcaccgg tggcaagcag ggattccagc
    15781 ttggcatctg tcatcggcgc cgccacgctc acgaccatgt ggtcgggatc atccaggaag
    15841 cgcaagcctt cggtcttggg aatatccttg agatggatac cttcattgat ctgcaattgc
    15901 gaagcgtcga cggcgatgaa gtccggcaac acagacggga gacattcgac atgcagttcg
    15961 cgcaaggcct gatgcagtac gccgccctct ttgaggccga tcggctgacc gccggtcaaa
    16021 tggataggca ccttcacgcg gatcggctta tccatggcga cttcgaagaa atccgcatgc
    16081 aacacgctgc tcgtgatcgg atcgacttga aactcgcgca acagcgccgt acggttgggt
    16141 ttacttttcg ccccgttgat cgtgagggaa atgagtgccg tggatccggc ttgtgaccgc
    16201 aaaactttga ccagttcatc cggcttgacg gacagcagga gacattctcc ctgcccatac
    16261 agaaccgccg gtacgcggcc ggcccgacga agttgacgcg ctgggccttt gcctccacct
    16321 tctctactct caacggttaa ttcgaatttc atgatgctcc tcctcgacct gtcgctccac
    16381 gcggacggcc tacacgaata acgaactcac tgattcatcc tcatggatac gcctgatggc
    16441 ttcgccaaac agcggcgcca cagacaacac tttcaacttc ggacacttct gctccttgcc
    16501 acgcaaggga atcgaatcgg tcaccagcac gtcagtcaat cccgattgtt ggagacgctc
    16561 caacgccggc ccagacaata cgccgtgcga acaaccggcc cacacctctc gcgcgccttt
    16621 atccaaacaa gcctgagcac cctgcacaat ggtgccggcg gtatcgatca tatcgtcaag
    16681 aagcaaggcg ctcttcccct gcacatcacc gataatattc atcacctgag cctgattcgg
    16741 tccctcccgc cgtttatcga tgatggcgag attggcctgc agacgtttcg caaaggctct
    16801 ggcacgttcc acgccgccag catcaggcga caccacgacc agatcctcga ttcttcgttt
    16861 ggtgatgtaa tccaatagca ccggcaaggc gtagagatta tccacgggta tattgaaaaa
    16921 accctgaatt tggctggcat ggagatccat cgtcagcacc cggtccgcgc cggcggtggt
    16981 aacaaggtct gccaccagtt tggcggaaat cggcacgcgc ggctgatcct tacgatcctg
    17041 cctggcatac ccgaaatacg ggatgaccgc ggtgatgcga ttggccgacg aacgcttgag
    17101 ggcatcgatc atgatgagca tttccatgat cgaatcattg accggcgcgc agcacgactg
    17161 aatgagaaac acgtctgccc cgcgcacatt ctcttcgatg cgaacgcgga tttccccgtc
    17221 actaaacgag gagaccgtgg ccgcgccgat gggcagatcg agatacgtgc cgatcgcgcg
    17281 cgcaagcgca ggattggcgt tgcccgaaaa gagcttcagt tccctgttca ttccacccct
    17341 tcgatgcggt acgtggcgat gccgatgctg taacggtgcg tatcgagtcg ttcaaaaacg
    17401 gtgggcacgg aacggaccgc tattaatcct gccgacctgg cactgtcagg acaagaggac
    17461 gcctaccgaa gcacagcaca ttaacgaaaa ccctgggaag ttgtcaataa tctgcgacgg
    17521 gctgggggcc gaggaaagtg agtgccggtc tgtcgtgacc gtggtgctcc agatagggtc
    17581 gtttcctaca cacgggagac cccgattcaa cctctatcag cgtctctctt cacaccaccg
    17641 tcgtcgtcgg agcgccggct gccggcaccg cataggccgt cattccggga ttgcgcccga
    17701 acacatccgc cgcctgctgt gcggcagact gctctcggaa caggccgaac atggtcgctc
    17761 cgctcccgga taacagggcc acctcggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnntct cggaacaggc cgaacatggt cgctccgctc ccggataaca gggccacctc
    17941 ggcgcccaag ctcagcaatt ggctcttaat ctgcgccagc accggatggt gagcgaacac
    18001 gggctcctcg aaatcgtttt ctaccaacgg aacaatctcc gaccaatcca gctcggagcg
    18061 ttgatccaag gactgcaccg ccaccgagag cggacgcaca ccggcgcggg tggcagccaa
    18121 ctgctgatac gcccatttgg tcggcacagg aaatcccgga ctgaccagca cgatccatct
    18181 gtttcccgtg actcggaccg gacggacatg ctctccccgt cccgtcacac aggcggcagg
    18241 cgccacaaaa aagaacggca catcacttcc taactgctgc cccacctccg ccatctgctc
    18301 gaccgaccag ttcaattcca acaaccgggt caacccgaga atggttgcgg cggcatcact
    18361 gcttcctcca ccaagtccgg cccccatggg aatgcgcttt gtcaggctga tggagagatc
    18421 catcgtccgg ttggctcgcg ccaacaccag ctccgcggca cgatagacca gattggtctg
    18481 atccaccgcc aggtcggcct ggttacagcg caaggtgatg cgggaggcgg cagcgggctg
    18541 catggtgagc atcaactcat cgttgagccc caccgtatgc atcaatgacc agatggcatg
    18601 gaaaccgtca ggccgtcgtt cgagcacccg gagaatgaga ttgaccttgg caggagtcag
    18661 aaccagcaga ctagtagagt tcactcgcag ctaacagggc ggcgctaatc ccggcctcct
    18721 ttgatttcat acttttcgag acggtaccgg aacgaccggg tattcaaccg cagcagtcgc
    18781 gcggctcgct ttttgaccca ctgactccgc tccagcgctt tcaacagcag atccttctcg
    18841 atcgtgttga tcaacccctc cagatccagg ccatcttccg gcaattccga gggcacagcc
    18901 tgctgggtgc tcactggctt atggagccat ccgcgaatat ccgcatccgt caccgacgat
    18961 cccgtggtga aggccacaac gcgttcgacg acgttctcca attcacgaac gttgccccgc
    19021 cactcatgcg ccaacagcac gcgcatggcc tcctggctca tcgtcggcac tggtttacca
    19081 ctttccttgg caaatttctc aagaaaatgt tggctcaata gcggaatgtc gccggtccgc
    19141 atgcgcaatg gcgggagcct gatcggaatc acatcgagcc gatagtagag atcctcacga
    19201 aatgcgcctt cggcaaccgc cttttccaag tcacgattcg tggccgccac gatacgcaca
    19261 tccactttca catcctgcgt gccgccgacc cgccggaact cccgctcctg aatgacgcgc
    19321 aagagcttga cctgaatcgc aggcgtggta tcgccgatct cgtcgaggaa aatcgtgccg
    19381 ccgttcgcca cctcaaacag accggccttg ttggaaaccg cgccggtgaa ggagcccttc
    19441 atgtggccga acagttcact ttcgagcagc gtttctggca ccgcgctgca attgaccgtc
    19501 acgaacggca tggaggctcg cgcactgttg aaatggatgg ctcgcgccac caactccttg
    19561 ccggtgccgc tttccccgcc gatcaggacg ttgctcctgg aatccgccac tttcttgatg
    19621 acatcgtaga ccttctgcat ggcctcgctc tggcccacga tctgggagaa cgacgactgg
    19681 ctggccaatt cccgcttcag gagcatgttc tccgtcgaca gccggcgccg ctcgatcgcg
    19741 ttgcgaatga tcaactgcac ttcatcgacc tggaaaggct tggtgagata gtcatacgcg
    19801 ccgtgcttca tggcttcgac tgcggaatcg gcggaggcaa aggccgtgat catcaacaca
    19861 acggtgtcag gcgatgaggc cttgacggcc ttgagcacgt ccaggccacc cattttcggc
    19921 atcttgaggt cggtgatgac gagatcaaaa atttctttat gaatctgcgc gatcgcctct
    19981 tccccgtccg acgccacggt tacggcgtac cccgcccgct tgagcatgat gctcaacaca
    20041 tcgcgcag
//
LOCUS       DAOQDS010000069        19683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1076_length_19683_cov_21.280518, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000069 DAOQDS010000000
VERSION     DAOQDS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19683
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1076_length_19683_cov_21.280518"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..623
                     /locus_tag="PK589_14830"
     CDS             363..623
                     /locus_tag="PK589_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HNJ20935.1"
                     /translation="MRKSIGVLLIFILIGGMLGGIFGEILRVMAPNGAIQNIFASNFS
                     PGINPPLTVDLVLLKLTFGFSIKVNLLSVLGMFLGAYLYKQM"
     gene            complement(666..2663)
                     /gene="uvrB"
                     /locus_tag="PK589_14835"
     CDS             complement(666..2663)
                     /gene="uvrB"
                     /locus_tag="PK589_14835"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNJ20936.1"
                     /translation="MPPFKLDAPFKPCGDQGQAIDKLTSGILAGKQHQALLGVTGSGK
                     TFTMANVVERVQKPTLVLVHNKTLAGQLYQEFKQFFPHNAVEYFISYYDYYQPEAYIP
                     QSDTYIAKDASINDAIDQMRHAATTSLLQRNDVLIVSSVSCIYGLGSPEVYHDMLVYL
                     EEGMETRREKILAKLVDIQYARNDVDFHRGTFRARGDVIEIFPASSEAKSVRIELFGD
                     TVDAIHEIDPLTGKSLGKLPKIAIYPNTHYLIAPDRYERAITGIEEELEARVAAFRKN
                     GQLLEAQRIEQRTKFDLEMIRAMGYCHGIENYSRHLSGRAPGDPPPTLLDYFPKDFLL
                     IIDESHATVPQIGGMYEGDYSRKRTLVDYGFRLPSAVDNRPLKFAEFEQMLKQVIYVS
                     ATPGPYELEHAKGEVIEQIIRPTGLMDPLIDVRSAKGQVDNLLAEVRAEAAKGNRVLV
                     TTLTKRMAEDLTEYYHDLGVKVRYLHSDIKTLERAEIIRELRCGVFDVLVGINLLREG
                     LDLPEVGLVAILDADKEGFLRSHRSLIQTAGRAARNLDGRVIFYGDTVTDSMRSAMDE
                     TARRRHIQEAYNVANGITPESIKKLIPVLDYASGGTNAGQLELAAESLADYAPTADTE
                     QLIRRLEVEMKAAAKKLEFERAAELRNRIRSLKLKALEFTE"
     gene            complement(2956..3489)
                     /locus_tag="PK589_14840"
     CDS             complement(2956..3489)
                     /locus_tag="PK589_14840"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ20937.1"
                     /translation="MSSARNLLLSMMVAAGCLAAGEGAFAQSDLAIADGMKVSLEYTL
                     SLPDKSVADSNVGQEPITFVQGAHEIVPGLEKALDGMKAGQKRRIDVSAQDAYGPYNN
                     KLRQSIDKDKLPKDVKVGDILQASDNRLVKVLEVNDKKVLIDLNHPLAGKTLTFDVNI
                     LKVEKGDAADVPEKKNP"
     gene            complement(3544..4893)
                     /locus_tag="PK589_14845"
     CDS             complement(3544..4893)
                     /locus_tag="PK589_14845"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNJ20938.1"
                     /translation="MVSFAELSLTSFLADRLQQAGFTAPTPIQSAAIPLALQGRDLLA
                     QAKTGSGKTLAFLIPLIERAVKEGWKPAGHASAQTGSSRSPRALVLAPTRELALQIEM
                     ELRKYAPPAVTSLAVYGGVPIERHYRALRQPPMIVIGTPGRLLDVAGTRHLDLRGIEY
                     VVMDEADQMLDRGFLRDIQRILQLLPSQRQTMLFSATFSPEILTLAESMLKNPARTAV
                     DPGVNTPTTITHAYYVVPSEASRVQLIHTLLQSSEAGDQSMVFCDQKYKVKRLAARLG
                     GEPASVGAITGNHSQAQRERTLTAFRSGRLRSLVATDVAARGLDVPTVSQVIHYELPG
                     NPTSYVHRTGRTGRAERSGATLLILSPQEEHEYLAMVRRLRIQTKRLTLPTLATLPPP
                     ARETESNSHPRHDGHGRDARPRRSGDRPNSTSQDARGGQGRRGWRAGRPAAPRRGNS"
     gene            complement(5041..5337)
                     /locus_tag="PK589_14850"
     CDS             complement(5041..5337)
                     /locus_tag="PK589_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNJ20939.1"
                     /translation="MGSKIYVGGLPYSTTEQQLSDLFAVHGAVTSARIITDKFTGQSR
                     GFGFVEMSGDSEAQAAINALNGTQFGGRTLTVNEARPQEPRSGGGGRGMGGGRH"
     gene            complement(5627..6457)
                     /locus_tag="PK589_14855"
     CDS             complement(5627..6457)
                     /locus_tag="PK589_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20940.1"
                     /translation="MHYGYQRVRQIQVGRKNQWGGVTSFVRALRYVGVAAVVGVLAGG
                     METAWAVPKETSTEKAEHLRQQANPQLFNDWTFDKDEVGAIPKGFVAVTSGDQRPGSW
                     KVEAQAAVPSAPNVLLGTSGCGECQEILVAQGFQYEYPDLVVRIQQGGGAATGQVGVV
                     FGMKDAQNYYATVVDVSQKIIQVVRVIEGKESVLGRAAIKPKPVEWHTLRVQRNTIIS
                     KDFVETFFDGSLALSVEDQALGVGQVGVLVRGESSARFDNFNAAPLYSSRPLSAPAAY
                     "
     gene            complement(6483..6728)
                     /locus_tag="PK589_14860"
     CDS             complement(6483..6728)
                     /locus_tag="PK589_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20941.1"
                     /translation="MAEQGKGLYDSQYARFRDNQGTHQGYEFQHGPAGSVPPPVVTFM
                     DKIKWWTLRGWRRKKILAERDRYQQDIIQIKTAHPKS"
     gene            6924..7361
                     /locus_tag="PK589_14865"
     CDS             6924..7361
                     /locus_tag="PK589_14865"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNJ20942.1"
                     /translation="MDLRQHPPRRWSDATAGLIWLPRLIDKVRAFQAGTLGAYAYPSA
                     LDQSFMRHLRLTPAQIESLVRDMTSDEAIGAALRQHIPLSTEEIQARCAAFQKKYEWA
                     FAVLDRDDGYTRGLGYPLPQFLQRPLWRWYQRWSAQKAHATSI"
     gene            7403..8944
                     /locus_tag="PK589_14870"
     CDS             7403..8944
                     /locus_tag="PK589_14870"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNJ20943.1"
                     /translation="MSAQSHHSNLVGQLILSLWIALTPVTVLAENQLEVPQSLRQSLE
                     LVSSQRMVEDVRTLSGPTFNGRQTGTSDDLASAEFVRQRFLESNSSQPPLQESRSKGS
                     PAHASIRIQSTSHRPTIIGKDSFLRLAGSADSPPDQIGPDYLPVLDSPSADVQAPVVF
                     VGYGISDPESGFDEYAGLDVRQKVVLFLRGKPERYAKQVSHADKAHAAHAHGALAYLT
                     ATGPILNAYETRRGVTGRPSAFYGLTEPDRTIPGAWISTTRAMAILHTDRTGHEDRLR
                     TLQQHLNEGQPPQSIATDTVVTMRWHSAVQDGLLHNVVLTVPGSDQAKAEETIVIGAH
                     RDHFGRQGGLLFPGADDNASGTAVLLEIARVLQAAPVQPKRSVVLISFSGEEQGLVGS
                     KFYISQPTAPLRSTSGMINIDHAAVGNGRLTIGVTGMDKAAAQQAGQTAGVADRLDVF
                     GFFPGGDHVPFKEAGIPTVTVVSGGIHPHFHQPTDTAETINPDILSEAARYVLAMLWQ
                     LADAP"
     gene            complement(8975..11140)
                     /locus_tag="PK589_14875"
     CDS             complement(8975..11140)
                     /locus_tag="PK589_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNJ20944.1"
                     /translation="MEEIISVNDQFYILASSSMADDRTRVLKHGETFGVFDRYGDIQP
                     VGRGTQGVFHQGTRFLSRQEIFLNNDRPMLLSSTVKEDNALLAVDLTNPDLYRDGRIA
                     IPRGSVHVFRSRFLWNGVSYERFRLSNYSLAPVKMTLSIRFEADFADIFEVRGKKRER
                     KGRMLPNILRKDLLVLRYEGLDEVTREARIQFVPEPLEITASQATFGIELEPKGEIAV
                     AVLVACDEVDSHRPLLSYDAAMAEAGLAFGAEQTEDCTIQTSNAQFNEWWNRSVLDVR
                     MMVTDTSEGPYPYAGVPWFSTPFGRDGVITAMECLWIRPELARGVLAYLASTQAKEVN
                     PAQDAEPGKILHETRKGEMAALHEIPFGLYYGSVDSTPLFVMLAGAYYERTADLPFIQ
                     SIWPNLEAALTWMDTYGDIDRDGFVEYVRKSPTGLDNQGWKDSHDSISHADGSLAEGP
                     IALCEVQGYVYDAKVQASKLAEALGYVDRASQLRRQARSLKERFEEAFWCEASSTYAL
                     ALDGHKRPCEVKTSNAGHCLYTGIASDEHARRVAETLMSDELFSGWGVRTLADSERRY
                     NPMSYHNGSIWPHDNAMIAVGLARYGLKAGVEKIMTGMFEVSLVLDFHRLPELFCGFV
                     RRPGQGLTRYPVACNPQAWAAGSAFMVLQACLGLSIVASEQKVIFNHPILPDFIDKMQ
                     IKNLTVGTASVDLLLRRHDLDVGITVIRRVGKVEVVSVK"
     gene            complement(11281..12624)
                     /locus_tag="PK589_14880"
     CDS             complement(11281..12624)
                     /locus_tag="PK589_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ20945.1"
                     /translation="MEPGRASGTGPGRPNVKWSPHLLTRDFTLVWWGQMVSQIGDGVS
                     KLALLWFVYSITGSPLKTTMIGLLQTLPPILFGPFIGVIVDRVPKKLLLISSDLIRAL
                     VLGVLPCLLPVDSFSIERLYLMVFVHAVASAVFGPALTAAIPSLVSRHEFTAANALLQ
                     TTTSIGIIVGPALSGVGIATMSSQEVLCVNAVSYVISALCFLLIRFPRTETVPAAEGG
                     SLAGTLRDVMEGFQYVLHRQRVILMLIGAASMYTFATSAFSTLFPVFGKKLLDLGPIE
                     VGYLWSAFGVGLLLVSLGLVSLSSWSLPKRIQLMAVSSFISGLTLLGLLFTSNRLVAA
                     VLMVIIGMGAGTLTPVAWGVLQEIAPTTLLGRVLAIYNLGAMTSAIAGMTIFGWVTQE
                     FGERPSVFGIGVGLFLSAVVSWRVARWVQVNWSETSVPAEEAARSVVMVTQPTSH"
     gene            complement(12680..13708)
                     /locus_tag="PK589_14885"
     CDS             complement(12680..13708)
                     /locus_tag="PK589_14885"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNJ20946.1"
                     /translation="MPMRTIQTVCVAGILSFVVASGLGSVRSMASEPQPSETKPDVAP
                     GSGSKDAEGKIKEPDTKPKEPESKPKEPEAKPTDQKSKDSDTKAKEPEPKAKDGEAKP
                     KGQEPKAKESTTKPKESEHKAKEGESKSSKEPDQKSKEPEAKPKDAEIAAEHPCPSVP
                     QAADAKPQVEAPVPSGDSGTGERQKSEPEPKKPVRSSMVTAKLALMADPHLFPYDIEV
                     DAKDKDLVLLGKVGQESDKRVATDIIRCLEGVHAVENRLKVEADAAHGLFAERDKIIT
                     QLVKERFEKSKTLQSVKFDVKTEDGVVTLNGATRFQIIVLEAAQAARQVPGVRAVNTD
                     AVRLVAGE"
     gene            complement(13910..15190)
                     /locus_tag="PK589_14890"
     CDS             complement(13910..15190)
                     /locus_tag="PK589_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ20947.1"
                     /translation="MADESQSTPESNVTGWRLLATRDFGCLWAGQVISQIGDGLNKVA
                     LLWFVYEMTGSALKMTAIGLLQTIPPLVFGPLIGVYLDYLPKKTVMIVVDLLRTLMVL
                     LIPLFYALDLLTLERLYVLVFLVSIVSTIFGPALASAVPLIVQRSQLTTANAFLQSTT
                     NIGVLLGPAMSGLGIALIGAQNVLYVDAATFLLSALFLFPIRVRETKSVKELDVLGTP
                     VMQDMMVGFRFVFLQHRVVFALMITAVLYNLAISAFVFLLPVVAKELLQVGPMELGWL
                     WSALGIGMLAASIWLARMPQGTFQERIGKIGRSLTIGGLAVCALGLIQTPVLFSTFLL
                     IIIIGGSTSLFYPVVWAMLQEVTPEHLLGRVFTTFSVGGMASAMVGMAGFGWAADTLG
                     PAVSLIGIGLLLLLTAMVTMQVSRRGLVVTPAVA"
     gene            complement(15224..16297)
                     /locus_tag="PK589_14895"
     CDS             complement(15224..16297)
                     /locus_tag="PK589_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ20948.1"
                     /translation="MRIAQVSPLWESVPPKLYGGTERIVSYLTEELVRQGHEVTLFAS
                     GDSVTKAKLESPCQQALRLNTGIFNREAPLIQMMEQVFAAADQFDLIHSHLDFLAFSL
                     SRRCRVPVVTTLHGRLDLPELVPVFRDFAELPLVSISDSQRRPLPWCNWANTVYHGLP
                     KDLYKFNAEPGKYLAFLGRVSPEKCPDQAIELAKRVGLPMKMAAKVDPADRAYFERVV
                     EPLLDHPLIEFVGEITDAEKSDFIGNAIGLVCPYDWPEPFGLVLIESLACGTPVLAYR
                     RGSIPEIIGHGVTGFISENLDEMVSQVSKLGTIDRHRCRQVFDERFTAQRMTNDYVKI
                     YQQLIADAATLPGKAGQQHASNL"
     gene            complement(16849..17307)
                     /locus_tag="PK589_14900"
     CDS             complement(16849..17307)
                     /locus_tag="PK589_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20949.1"
                     /translation="MMVAWYHKRRSVSMLARKGRATMRSTGIMLVAVTAALLWVSPAA
                     AQSVSGVRGFNGGVAPLFGLVGPGNLYIDNQGTQGFIYNFGNNFESYNFRNPTTGQAW
                     SGAVMTLGPQLSVGLIQGANQSGSPVVFPPVPRETAPLPPIQSGLLEDIP"
     gene            17356..18906
                     /locus_tag="PK589_14905"
     CDS             17356..18906
                     /locus_tag="PK589_14905"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ20950.1"
                     /translation="MTNPMLRGCLLIGGATALLLWTASTSFGLEVTKPVPTERREAMS
                     LADAVLKALQNNLDIHIGRQTKESRLADIIIEQAKFDPTISLNGQYNRQVAPLNRPIL
                     GFTGANLQDITKFDQNTSSVTADITQNLPTGANYDLNYSPQRSYVSGPNTFLFNPAWT
                     GGLALTVTQPLLRNFGTDVNRTFISIAQNNATVEQHVFLDRVLTVIATVEQTFWEMVF
                     ANENLKVAQAALKAAEELLASNRAKAKAGVMSIVDVLQAEAAVASRVEQILVAEKSIR
                     DQEDQLRRLLNPAEEELRQDLRLMPTDPPTTSLEAISLQEAIDIAMERRPEVLQAGKN
                     VESSDLNVKFAKNQLLPTLSVQGTMGLSGLGADYGDATRRNFGGDFYNYGAGLVLSYP
                     IGNRSAYSTYNKRQLESRNAQASLQSVRQQVIVGVREAVRRVHTDFKRIETTRSARIM
                     AEKQLQAEQERLKVGLSTTRFVLDFQRDLATAQGNELRATVDYNKSLSNLARNKATTL
                     ERYSLRLE"
     gene            18913..19596
                     /locus_tag="PK589_14910"
     CDS             18913..19596
                     /locus_tag="PK589_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20951.1"
                     /translation="MIRPEEASAAYPIQHNERGAALALLPLIATLLYYASPRELRQEP
                     LYQFLPQLCAYAALLTWSRLNGATVTRLGLTRALIPRGLRLGIATGLALGTVNLLVIL
                     YLVPWLGADYRFLAGTPHAKIPLLLMLPWFILMIAAMVELNFRGFLLGRLLGLGLPSA
                     LAVPLSALLFAFDPFLVATFHHLHWIAVWDGLIWGSLWVAFRNLYVTIVAHAVEVIVL
                     YSVMRTILT"
BASE COUNT         4339 a         6095 c         5280 g         3969 t
ORIGIN      
        1 tcgagccaga gctccgatcg cctcgaaaaa catcaaaaaa atagcatcgg gcagtctata
       61 gaagggcggc gcgtgaagtc aagatattcg gcactctatt ggcgacgtcc ggaagactcg
      121 ccacgtcacc tttctcgaca tcaccgccag ccctcggcca gccaaaaacg gtcgtgatat
      181 agggcgcggg accgaacggc gaagaaagaa ttccaccgtg gagcacggca gggcctttcc
      241 cgccaggaac atgcgccact gctagcatct ggcatgcatt ttgttgacat cattttacgc
      301 ttccgatatg gtgcctcaag tcggcacata ccgccgacag tgtgaaccgg gagagacatc
      361 ggatgagaaa atcgatcggc gtgctgctga tctttatcct gatcggcgga atgctgggcg
      421 ggatttttgg tgaaattctg cgcgtgatgg ccccgaacgg cgcgattcaa aacatctttg
      481 ccagcaattt ctctcccgga atcaacccac ccctcacggt ggatctggtt ctcctcaaac
      541 tgacctttgg tttcagcatt aaggtcaatc tgcttagcgt gttaggaatg ttcctgggag
      601 cctatctcta caagcagatg taagttgagg ccattcccaa ccgtcgccgt ctcgcagctc
      661 gatcgttact cggtgaactc cagcgctttc agcttcaagg acctgattcg gttccgcaac
      721 tctgccgccc gttcaaactc caacttcttg gcggcggctt tcatctcgac ctccagacgc
      781 cggatcaatt gctccgtatc cgctgtgggc gcatagtccg ccaacgattc cgccgcaagc
      841 tcaagctgac cggcattcgt tcctcccgac gcatagtcga gcaccgggat cagtttctta
      901 atgctttccg gcgtaatgcc attcgcgaca ttgtatgcct cctggatatg gcgccggcgc
      961 gccgtctcat ccatcgcgct ccgcatcgag tcagtcaccg tatccccata aaaaatgact
     1021 cggccatcga gatttctcgc cgcgcggccg gctgtttgaa tcagagaccg atgagaccgg
     1081 agaaatcctt ccttatccgc atccagaatc gccactaatc cgacctccgg aaggtccagt
     1141 ccctctcgca acaggttaat cccgaccagc acatcgaaca cgccgcatcg gagctcgcga
     1201 ataatttccg cccgctccag cgttttaatg tccgagtgca gataccgcac tttcaccccg
     1261 aggtcatgat aatactcggt gaggtcttcg gccatgcgct tggtcaaggt cgtcacgaga
     1321 acgcgattgc ccttcgctgc ttccgcccgg acctccgcca acagattgtc cacctgcccc
     1381 ttcgccgatc gcacgtcgat cagaggatcc attaagccgg tcgggcggat gatctgctcg
     1441 ataacttcac ccttcgcatg ctccagttca taggggcccg gcgtcgccga gacatagatg
     1501 acctgtttga gcatctgttc gaactcagca aacttcagcg gacgattgtc gacagcagac
     1561 ggcagccgga agccatagtc cacaagcgtg cgtttcctgg aatagtcacc ttcgtacatg
     1621 ccgccgatct gcggtaccgt cgcgtgcgat tcatcgatga tcaagagaaa gtctttgggg
     1681 aaataatcga gcaacgtcgg cggcggatct cccggtgcac gcccgctcaa atggcgcgaa
     1741 tagttttcga tcccgtggca ataccccatg gcacgaatca tttcgagatc gaatttggtt
     1801 cgctgctcga tccgttgcgc ctccaataac tgaccgttct tcctgaacgc ggccactcgc
     1861 gcctcaagct cctcctcgat accggtaatc gcccgttcgt aacgatcggg cgcaatgagg
     1921 tagtgcgtat tggggtagat ggcgatcttc ggcagcttgc ccagcgattt tccggtcagc
     1981 ggatcgatct catggatggc atcgactgta tcgccgaaca gctcgatgcg caccgacttg
     2041 gcttccgacg aagccgggaa aatctcgatg acatcgccgc gcgcgcgaaa tgttccacgg
     2101 tggaaatcca cgtcattgcg tgcatattga atatcgacca gtttggccag aatcttctcg
     2161 cggcgcgtct ccatcccctc ttcgagatag accaacatgt catggtagac ctccggcgac
     2221 ccgaggccgt agatgcagga aacggacgag acgatcaaga catcattccg ctgcaggagc
     2281 gatgtcgtgg cggcatggcg catctgatcg atagcatcgt tgattgaggc atccttcgca
     2341 atatacgtgt cgctctgcgg aatgtacgct tccggctggt agtaatcgta gtaactgatg
     2401 aagtactcga cggcgttgtg cggaaaaaac tgtttgaatt cctggtagag ctgcccggcc
     2461 aacgtcttat tgtgaacgag cacgagagtc ggcttctgca cccgctcgac gacattcgcc
     2521 atcgtgaagg tcttgccgga accggtgacg ccgagcaacg cttgatgctg cttgccggcc
     2581 aaaattccgg acgtcagctt gtcaatcgct tggccttggt cgccgcaggg tttgaatgga
     2641 gcatcgagct taaacggggg cataatcacg tcatcttaac atgcgcgcca gctaaatcgc
     2701 ccggcctgaa aacccgatga agtttgccga ctgtcgatgt gaccgagcag caggacgcca
     2761 tgatcttcgg ggcatgtctc gaagacacgc cacctctgaa atgacatcgc gtttacatcg
     2821 ttgaagggag actcgaacgc acactgtgcc gccgtgacgc tgatcgccac ggcgagtcgg
     2881 agggcatctt accgcgagaa cctacaacca gcccgatcag cgggcctgct caccgcttag
     2941 gtgcattgcg atcggtcagg gatttttctt ctcaggaacg tcggcggcat cgcctttttc
     3001 caccttgagg atattcacgt caaacgtgag tgtctttcct gccagtggat gattgagatc
     3061 gatcagaact ttcttgtcat tcacttccaa gactttgacc agccggttgt ccgaagcctg
     3121 caaaatgtcc ccgaccttca cgtccttggg aagcttatcc ttatcgatac tttgccgcag
     3181 cttattgttg taggggccgt aggcgtcctg tgccgacaca tcgatacgcc gcttctgtcc
     3241 ggccttcatg ccgtcaagcg ccttttcgag gccgggaacg atttcatgcg ccccctgcac
     3301 aaacgttatc ggctcctgcc cgacattcga atcggccacg cttttgtcgg gaagcgacaa
     3361 cgtatactcc aacgacacct tcatgccgtc ggcaatggca agatcgcttt gcgcgaaggc
     3421 gccctcgccg gctgcaagac aaccggcggc gaccatcatg ctcagcaaga gattccgtgc
     3481 agaactcatc gcggcatcct cctcaatctg ccggacagca tttccatccg gccaaatact
     3541 gcatcaacta ttgccgcggc gcggagcggc aggacgaccg gcacgccatc cgcgacgtcc
     3601 ctgtccccct ctggcgtcct ggctcgtgga gttagggcga tctccggaac gacgcggccg
     3661 ggcatcacgc ccgtgtccat catgccgggg atggctgttc gactccgtct cacgcgcggg
     3721 aggcggcaac gttgccaagg tcggcagtgt caggcgcttc gtttggattc gcaaccgtcg
     3781 caccatggcc aaatattcat gctcctcttg cggagagaga atcaagaggg ttgcgccgga
     3841 tcgctccgct cggcccgtcc ggccggtacg gtgaacgtac gaagtcgggt tgccaggcaa
     3901 ctcataatga atgacctgcg aaacggtcgg aacatccaat ccgcgcgccg caacatcggt
     3961 ggccaccaac gaccgcaacc gaccagaacg gaacgccgtc agcgtccgct cacgctgcgc
     4021 ctgcgagtga ttgccggtaa tagcccccac cgaagccggt tctccgccca accgggcagc
     4081 caggcgcttc actttgtact tctggtcaca aaacaccatc gactgatcac cggcttccga
     4141 tgattgcagc agcgtatgaa tcagctgcac ccgcgatgct tcgctgggca ccacgtaata
     4201 ggcatgcgtg atggtggttg gcgtgttgac gccgggatcc accgccgtcc gagcaggatt
     4261 cttcagcatc gattctgcca gcgtgagaat ctccggcgag aacgttgccg aaaacaacat
     4321 ggtctgccgc tgagagggaa gcagctgtaa aatacgttgg atgtcgcgca ggaacccccg
     4381 atcgagcatt tgatcagctt catccatcac gacatattcg atgccgcgca aatccaaatg
     4441 ccgagtgccg gccacatcca gcagacgccc cggcgtcccg atgacgatca tcggcggctg
     4501 acggagggca cggtagtgcc gctcgatggg cacaccgccg tacacagcca gcgacgtcac
     4561 cgccggtggc gcatacttgc gcagttccat ttcaatctgg agggccaact cacgggtcgg
     4621 cgccaacacc agggcacgcg gcgaccgtga cgacccggtt tgcgcggacg catggccggc
     4681 aggcttccac ccttccttga cggcacgttc aatcaacgga atgagaaacg ccaacgtttt
     4741 cccgctcccc gttttggcct gggctaacag atcacgcccc tgcagcgcca gaggaatcgc
     4801 agcgctctga atgggagtcg gcgcggtaaa cccggcctgc tgtaaacggt cagcgagaaa
     4861 cgatgtaaga gacaattcgg caaacgacac catgcaacga cactcctaaa tctctatctg
     4921 gggaaacagg ggcagacaac ctgatggccc tgcagaccaa agagaggaca acacggtaag
     4981 aaaaacgcag gggctaccct gtcggcagcc cctgaatagg tatatagaaa cgggcgcagc
     5041 ttagtgccgg cccccgccca ttccacgtcc accgccaccg gagcggggct cctgcgggcg
     5101 ggcttcgttg accgtcaagg tgcggccacc gaattgtgtc ccgttcaatg cattgatggc
     5161 cgcctgtgcc tcagaatctc ccgacatttc cacgaaacca aagccccgtg attggccggt
     5221 gaacttgtcc gtgatgatgc gcgcagacgt caccgcgcca tgcaccgcga acaggtcact
     5281 cagctgttgt tcggtggttg aatatggcaa gccgccaacg tagatcttcg aacccattgg
     5341 gttcctcctt tgagaataga atggtgttgt ctgggactcg agaaagaaac gaggaaggaa
     5401 tggggcgaag atgcaaacac agcggcaatc ttagctctgg cttccgaaat tcccgggaag
     5461 aacccaaaac aacatcgaca cgagccctgt aattcttttt ctacttcatc gccaaggctc
     5521 ttcgcaatga agcaccttca ctgtatcgca ccaaaatggg aaaggcaagc gcgaaatcgt
     5581 ctcgccaaaa atctatccga tccggccgaa tgtcactttt gctggctcaa taggccgccg
     5641 gagcagacaa cggtcggctg gaatataagg gggccgcatt gaaattatcg aagcgtgccg
     5701 acgactcccc cctcaccaat acccccacct gccccactcc aagcgcctga tcttccacgg
     5761 acaaggccag acttccgtca aagaatgttt cgacgaaatc cttgctgata atggtattcc
     5821 gctgtacgcg aagggtgtgc cattcaaccg gcttcggctt gattgccgcc cggccgagga
     5881 ctgactcttt cccctcgatc acgcgcacaa cctgaataat tttctgagac acatccacga
     5941 cagtcgcgta gtaattctgg gcatccttca tcccaaacac gacacccacc tgcccggtgg
     6001 cagccccgcc cccctgttga atgcgaacga ccagatcggg gtactcatac tgaaatccct
     6061 gcgcaacaag aatttcctga cactctccgc agccggatgt cccgagcaac acattgggag
     6121 cagacggcac ggctgcttgc gcctcgacct tccacgaacc tggccgttga tcgccggagg
     6181 tcacggccac gaaccccttg ggaatggccc cgacttcatc tttatcaaac gtccaatcgt
     6241 tgaagagttg gggattggcc tgttgccgca aatgctccgc tttttccgtg gaggtttcct
     6301 tcggcacagc ccatgccgtc tccatcccac ccgccaacac acccacgacg gccgccacgc
     6361 caacatatcg cagggccctg acaaacgatg tgacaccacc ccactgattt ttccgaccta
     6421 cctgtatctg cctgacgcgc tgatatccat agtgcacgga agtactcctc ctcgagtgga
     6481 cctcacgatt tcggatgtgc tgttttgatc tggatgatgt cctgctgata tcgatcacgt
     6541 tcagccaaga tcttcttccg tcgccatccg cgtaacgtcc accattttat tttatccatg
     6601 aacgtaacca cgggcggagg cacactgcct gccggcccgt gctgaaattc atagccctga
     6661 tgtgtcccct gattgtctcg aaaccgtgcg tactgagagt cgtagagacc ttttccctgc
     6721 tctgccatag ctgattccct ttgcgttatc agtcgagctg ctgttgcgat ccgggaccgc
     6781 ctcatgcagc ggaatactga tcctttgccg gcccgggaac cgatagtacc ttgagccctt
     6841 tgctctctgc aacggggctc ttcccttatg ctatagtgcc tcattccgaa ggccgcctca
     6901 ttgagctgaa acctggatca cgcatggatc ttcgacagca tccaccccgc cgatggagcg
     6961 acgccacggc cggattgatc tggttacccc gcctgatcga caaggtgcgg gcatttcagg
     7021 ccggcacgct cggcgcctat gcctaccctt cagcattgga ccagtccttc atgcgccacc
     7081 tccggctcac gcccgcccag atcgagtcgc ttgtccgtga catgacctcc gatgaggcga
     7141 tcggggcggc ccttcggcaa cacattccac tcagcaccga agaaatccag gcgcgctgcg
     7201 cagcctttca aaagaaatat gagtgggcgt tcgccgtact cgatcgagat gacggctata
     7261 ctcgaggact gggatatccg cttccacagt ttctgcaacg acccctctgg cgctggtatc
     7321 aacgttggtc ggcccagaaa gcacacgcca cttcgatctg acgccattcg ctgacgtagg
     7381 tcctcacagc aggagagcct cattgtccgc ccaaagccat cactccaatt tagtgggtca
     7441 actcatcctc agcctctgga ttgcgctgac gcctgtgacg gtgctggccg agaaccagct
     7501 ggaagttcct cagtcgctca gacaatcctt ggagctcgtt tccagccaac gcatggtgga
     7561 ggatgtccga acactgagtg gaccaacctt caatggccgc cagaccggca cctccgacga
     7621 cctggcctcc gctgaattcg tacggcaacg cttcctggaa tcgaacagct cacagcctcc
     7681 cttgcaggaa tctcgcagca agggttctcc cgcccatgca tcgattcgca tacagtccac
     7741 ttcccatcgg ccaacgatca tcggcaagga ttcgttcctg cgcctggcgg gcagtgccga
     7801 ttcgccgccg gaccagatcg gccccgatta ccttcccgtc ctcgactccc cttcggccga
     7861 cgtgcaggcg ccggtcgtgt ttgttggata cggaatctcc gacccggaaa gtggcttcga
     7921 cgaatacgcg ggcctcgatg ttcgtcagaa ggtcgtgctc tttttgcgtg gcaaacccga
     7981 gcgatacgcc aagcaggtgt cacatgcgga taaggcccat gccgctcacg cccatggggc
     8041 gctggcctac ctcactgcca ccggaccaat cctcaatgct tatgaaactc gccgcggagt
     8101 caccggtcga ccgagcgcct tttacggctt aacggagccg gatcgtacga ttcccggtgc
     8161 ctggatcagc accacccgcg cgatggccat actccacact gaccgaacag gccacgagga
     8221 tcggctacgg acgttgcagc aacacctcaa tgaaggccag ccaccgcaat cgatcgcgac
     8281 ggataccgtc gttaccatgc gttggcacag cgcagtccag gacggcctgc ttcacaacgt
     8341 cgtcttgacc gtaccgggaa gcgaccaggc gaaggcagaa gaaacgatcg tgatcggtgc
     8401 gcaccgcgat cattttggac gccaaggcgg cctgctgttc ccaggagccg acgataacgc
     8461 ctccggaaca gccgtgctgc tggagatcgc gcgcgtgctg caagccgccc cggtccagcc
     8521 gaaacggtcg gttgtcctga tttcgttcag cggagaagaa caggggttgg tgggatccaa
     8581 attctatatc agccagccga ctgcgccctt acgttcgacc tcggggatga tcaatatcga
     8641 ccacgcagcg gtcggcaacg gacgcctgac gatcggtgtc accgggatgg ataaggctgc
     8701 cgcgcagcag gcggggcaaa cggcaggcgt agcagaccgc cttgatgtct tcggcttttt
     8761 ccccggcgga gatcatgtgc cgttcaagga ggcgggcatt cccacggtca cggtggtgag
     8821 cggcggaatt catccccatt tccaccagcc cacagacacc gccgagacta tcaaccctga
     8881 tattctttca gaggctgccc gatatgtcct cgccatgctg tggcagctgg cagacgcgcc
     8941 ataacgcagc ggagcgggcc acgtattgcc cgcgctactt caccgaaacc acctcgactt
     9001 taccgacccg ccggatgacc gtgatcccga catcgaggtc atgccgccga agcaacaggt
     9061 ccaccgacgc ggtgccgacc gtcagatttt tgatctgcat cttgtcgata aagtccggaa
     9121 gaatcggatg gttgaagatc actttctgtt ccgacgcgac aatcgacaag cccaggcagg
     9181 cctggaggac cataaaggcg gatccggccg cccaggcctg cggattacag gcgacaggat
     9241 atcgggtgag gccttggccg ggacgacgga caaacccgca gaacagttcc ggcaaccggt
     9301 gaaaatcgag gacgagactc acctcgaaca tcccggtcat gattttttcc acgccggcct
     9361 tgaggccata ccgcgcgaga ccgacagcga tcattgcatt gtcatgtggc cagatcgatc
     9421 cgttgtggta ggacatcggg ttataccgac gttcggaatc ggccagggtc cgcacgcccc
     9481 acccgctgaa taactcatca gacatgagcg tctccgcgac acgccgcgca tgctcatcgc
     9541 tggcaatgcc tgtgtagaga cagtgaccgg cgttcgacgt cttcacctcg caggggcgct
     9601 tgtgcccatc cagggcaaga gcataggtcg acgaggcttc gcaccagaag gcctcctcga
     9661 accgttcctt gagtgaccga gcctgccgtc gcagctgact ggcacgatcg acgtagccca
     9721 aggcttccgc cagtttcgac gcctgaacct tggcgtcata cacatagccc tgcacttcgc
     9781 acaacgcgat cggtccttcg gccaaggaac cgtccgcgtg cgagatggaa tcatgggagt
     9841 ctttccatcc ctggttatcc aagccagtgg gcgatttccg cacatactcg acgaaaccat
     9901 cgcgatcgat atccccatac gtatccatcc aggtcaatgc cgcttccaga ttgggccaga
     9961 tggattgaat aaagggcagg tctgccgtgc gctcgtaata ggcccccgcc agcatcacga
    10021 ataacggggt ggaatcgacg ctgccataat agagcccgaa gggaatttcg tgcagcgccg
    10081 ccatttcgcc cttgcgggtt tcgtgaagaa tcttccccgg ctcggcatcc tgagcaggat
    10141 tgacctcctt cgcttgagtg gacgccagat aagccaatac cccacgcgcc aattccggtc
    10201 tgatccacaa acactccatc gccgtgatca cgccatcccg cccgaagggg gtgctgaacc
    10261 agggcacacc ggcgtaggga tacggacctt cactcgtatc ggtgaccatc atgcgaacgt
    10321 ccaacacgga acggttccac cactcattga actgcgcgtt ggacgtttgg atggtacagt
    10381 cttccgtctg ctcggcgccg aatgccaaac cggcttctgc catcgccgca tcataagaca
    10441 agagcggccg atgactgtcc acctcatcgc aggccaccag gaccgctacg gctatctccc
    10501 ctttcggttc cagttcgatg ccgaaggtgg cttgtgacgc ggtaatctcc aacggctccg
    10561 ggacaaactg aatccgggcc tcccgcgtca cttcgtcgag accttcgtat cgcagcacca
    10621 ggagatcttt ccgtaagatg ttcggcaaca tgcggccttt gcgctcgcgt tttttaccgc
    10681 gcacctcaaa gatgtcggcg aagtcggcct cgaatcggat cgagagggtc atcttcactg
    10741 gcgccaggct gtagttcgac aaacgaaacc gctcataact gacgccattc cagagaaaac
    10801 gagagcgaaa gacgtgtacg ctgccgcgcg ggatggcgat gcgtccgtcc cgatacaagt
    10861 ccgggttggt cagatccacg gccagcaagg cgttgtcttc ctttaccgtc gagctcaaca
    10921 gcatcggccg atcattgttc agaaatattt cctgccgcga gagaaaccga gtcccttgat
    10981 ggaacacgcc ttgtgtgccc cggcctaccg gttgaatgtc gccataccgg tcaaacacac
    11041 cgaaggtttc tccatgcttg agcacacgcg tccggtcatc ggccatcgat gaactggcca
    11101 ggatatagaa ctgatcattc acgctaatga tttcttccac gttttcctcc cgtcatccct
    11161 gaacgatcga tgcaatgggc gcggcctgtg ccctcacacc tcaccaattc ctgcctgctg
    11221 ctcgcttcgt ctttggacag ggcctctcga cccgtgcaat catatgatac ggatgtggac
    11281 tcaatgactt gtgggctgcg tcaccataac aacggaccgg gcggcttctt cggcaggcac
    11341 gctcgtctcg gaccaattga cctgcaccca tcgggccact cgccaggaca ccaccgccga
    11401 taaaaacaga cccaccccga ttccaaatac gctgggacgc tcaccgaatt cctgcgtcac
    11461 ccagccgaaa atcgtcatgc cggcgatcgc agaggtcatc gccccaagat tgtagatagc
    11521 cagcactcgc cccaataacg tcgtgggcgc aatttcctgc aagacacccc aggccaccgg
    11581 ggtcagcgtg cccgcgccca tgccgatgat gaccatgagc acggcggcta ccaaccggtt
    11641 cgatgtgaag agcaaaccca gaagggttag gccgctgatg aaactggaca ccgccatcaa
    11701 ttgaatccgc ttcggcagcg accaggacga cagcgacaca agtcccagcg agaccaagag
    11761 caatcccaca ccgaaggccg accacaaata cccgacttcg atgggcccca gatcaagcag
    11821 ctttttgccg aacaccggaa acaacgtgct gaacgcacta gtggcgaagg tatacatgga
    11881 ggctgcgccg atgagcatga ggatgacccg ctgccgatgg agcacatact gaaacccttc
    11941 catcacgtcc cgcagggttc cggccaggga gcccccctcc gcagccggca ccgtttccgt
    12001 gcgcggaaag cggatcaaca aaaagcacag cgctgatatc acataactga cggcattcac
    12061 gcagagcact tcttgagaac tcatcgtcgc aatgccgacg ccgctcagcg ccgggccgac
    12121 gatgatgccg atgctggtcg tggtctgcag caaggcgttg gccgccgtga attcatggcg
    12181 ggacaccagg gaaggaatcg cggccgtcag ggcaggaccg aacacggcag acgccaccgc
    12241 atgaacgaac accatgagat acagccgctc aatgctgaac gagtctaccg gcagcaagca
    12301 gggcaacaca cccagcacta acgctcgaat caagtcgcta ctgatcagca ggagtttttt
    12361 ggggacacga tcgacgatca caccgatgaa gggaccgaac agaatgggcg gtagcgtctg
    12421 caacagcccg atcatggtgg ttttcagagg agagccggta atggaataga cgaaccagag
    12481 cagcgcgagt ttcgacacgc catcgccgat ctgtgagacc atctggcccc accagaccag
    12541 cgtgaaatcg cgcgtcaaca gatgcggcga ccacttgacg ttggggcgtc ctggcccggt
    12601 gccgcttgcg cgtcccggtt ctatagcgtc agcagactgc gtgtcgaacc tcctgtggca
    12661 tcggcctcat gaaccgcgct tactctccgg cgaccaagcg gaccgcatcg gtattcaccg
    12721 ctcggacacc cggtacctgc cgtgccgcct gtgccgcttc gaggacgatg atctgaaacc
    12781 gcgtcgctcc gttcagggtg acgacgccgt cctctgtttt cacatcgaac ttgaccgatt
    12841 gcaaggtttt gcttttctcg aaccgctcct tcaccaattg ggtaatgatt ttgtcacgct
    12901 ccgcgaacaa cccatgcgcg gcgtccgcct cgactttgag ccggttttct acggcatgca
    12961 cgccttcgag gcatcgtatg atatcggtgg ccacacgctt atccgactcc tgcccaactt
    13021 ttcccaacaa gacaaggtct ttatccttgg catccacttc gatatcgtag ggaaacagat
    13081 gggggtcggc catcagggcc aacttggccg tcaccattga tgagcggacc ggcttcttgg
    13141 gttccggttc cgatttctga cgctctcccg taccggaatc accagaaggg accggcgctt
    13201 ccacctgcgg cttcgcgtca gccgcttgcg gcaccgaggg acagggatgc tcagcggcaa
    13261 tctcggcatc cttcggtttc gcttccggct ccttggattt ttggtccggc tctttcgagg
    13321 atttggactc accctccttc gccttatgtt ccgattcctt gggcttggtc gtcgattcct
    13381 tggctttggg ttcttgacct ttgggttttg cttccccgtc cttggccttc ggttcaggct
    13441 ctttcgcctt cgtatcgctg tcctttgatt tttggtcagt cggcttggcc tccggctctt
    13501 ttggtttgga ctccggctct tttggtttcg tatccggttc ctttatcttt ccttcggcat
    13561 ccttcgatcc cgaaccaggt gcaacgtcgg gttttgtctc tgacggctgc ggctccgagg
    13621 ccatcgacct cacgctccca agcccggacg ccaccacaaa cgacaaaatc cctgccacac
    13681 acacggtctg aatagtccgc ataggcatat gcccctcatg tgtaaaagta aaccgagccg
    13741 agaaggcagg cccggattcg tcttcgaaca gcctgagaga aaagacatac gcgacggagg
    13801 ggcagaaagc aactccgccc atcgccggca ttggtcaagc ggggtgagct cgcgtggcgc
    13861 ataccagcga gctgccgcat agaccgaaac gatcgtcctg gaagggaggt tatgcgacag
    13921 ccggagtcac caccaggcca cgacggctaa cctgcatcgt gaccatggca gtgagcaaga
    13981 ggagcagtcc gataccgatg agactgacgg caggcccaag ggtgtcggcg gcccagccga
    14041 atcccgccat gcccaccatg gccgacgcca tcccaccgac actaaacgtg gtaaagaccc
    14101 gcccgagcag atgttcgggc gtcacttcct ggagcatggc ccacacgacc gggtagaaca
    14161 gggaggtgct accaccgatg atgatgatca ataaaaacgt gctgaacaac accggagttt
    14221 gaatcaatcc caacgcgcag accgcgaggc ccccgatcgt caacgagcga ccgatcttgc
    14281 cgatacgctc ctggaatgtg ccctggggca tccgagccaa ccaaattgat gccgccagca
    14341 tcccgatgcc tagggcagac cacaaccatc ccaactccat cggtccgact tgcaacagtt
    14401 cctttgccac cacagggagg agaaacacaa aggcgctgat cgccagatta tagaggacgg
    14461 ccgtaatcat caatgcaaac accactcggt gctgcaggaa gacgaagcgg aatcccacca
    14521 tcatgtcctg catgacggga gtccccagga catccagctc cttcaccgat ttggtctctc
    14581 tcacacgaat cgggaataag aagagggcgg agagcaggaa ggtggcagca tccacataca
    14641 gcacattttg cgcgccgatc aaagcgatac ccagaccgct catggccggt cccaacaaga
    14701 cgccgatgtt cgtagtgctc tgcagaaacg cattcgccgt cgtcagttgc gaccgctgga
    14761 caatcagagg cacagccgag gccaacgccg gtccgaatat agtggacaca atggagacca
    14821 ggaagacgag cacatagagg cgctcaagcg tcaacagatc cagcgcgtag aagagcggta
    14881 tcaacagcac catcaacgtt cgtaacagat cgacgacgat catgaccgtt tttttcggga
    14941 gatagtcgag atagacgccg atgaggggac cgaagaccag aggcggaatc gtctgcaata
    15001 acccgatggc ggtcatcttg agcgccgacc cggtcatttc atacacgaac cacagaagcg
    15061 caaccttgtt cagtccgtca ccgatttggg agatgacttg gccggcccag agacatccaa
    15121 agtctcgggt cgccaacaac cgccatccgg tgacattgga ttcaggagtg gactgcgact
    15181 cgtctgccat actggtcctt ccgcgaggtt gagatcgcgc tgactagagg ttcgaagcgt
    15241 gctgctgccc ggctttaccc ggaagcgtag cggcatccgc aatgagttgt tgatagatct
    15301 tgacgtaatc gttggtcatg cgctgggcgg tgaaccgttc atcaaagacc tggcgacatc
    15361 gatggcgatc gatcgtgccc agcttcgaca cctggctcac catttcatcg aggttttcac
    15421 tgatgaagcc cgtcacacca tgaccgatga tttctggaat agatccccgg cggtaggcga
    15481 ggacgggtgt accacaggcg aggctttcga tcaatacgag gccgaacggt tcaggccagt
    15541 catagggaca gacgagcccg atggcgttcc cgataaagtc gctcttttct gcatcggtga
    15601 tttctccgac gaactcgatc aacggatggt cgagcaacgg ctccaccact cgctcaaagt
    15661 aggcacggtc tgcgggatcg accttcgcgg ccatcttcat gggcaagccg acacgtttgg
    15721 ccaactcaat ggcttgatcc ggacattttt ccggagagac gcggccgagg aaggccaagt
    15781 acttgcccgg ttcggcgttg aacttataca gatctttcgg caacccgtga tagacggtgt
    15841 tggcccagtt acaccagggc aagggccggc gctgcgagtc cgaaatggaa accagcggca
    15901 gctccgcaaa atcacggaag accggcacaa gctcgggcaa gtccaaccgc ccgtgaagcg
    15961 tggtcaccac cggcacgcga cagcggcggg agagggaaaa ggccaaaaag tcgagatggg
    16021 agtggatgag atcgaactgg tcggcggcag cgaacacctg ctccatcatc tgaatgagcg
    16081 gtgcttcgcg gttgaaaatg ccggtgttga gacgcaacgc ctgctgacac ggggattcca
    16141 acttcgcctt ggtcaccgaa tcaccgctgg caaacaacgt cacctcatga ccttgacgga
    16201 cgagttcttc cgtcaagtag gagacgatgc gctcagtgcc tccatacaat ttcggaggga
    16261 cgctctccca aagcggcgat acctgggcaa tcctcataca aacaatctcc tttgatcgta
    16321 gatgctcggt gacgggggct gaaaaccggc ggctggcact tcaccgcgcc tcccaccgtc
    16381 tgtcggcatt ctttccgttc tccccatgcg attcaattga catctttgac aggagcgcat
    16441 tgtttttgtc gtctcgttac gaaacaggag atcgatcgca atgacgcgga gtcaaagcaa
    16501 gcgccatacc gtcgatgact tcctcatcaa ttggcaatgc atacaatcaa cacgtacgtt
    16561 ataaatcaac gccttacgaa tatcctcccg ccactttgca acgatcgaca ggtgacgttc
    16621 aggctgtgga ttctgtggat aatgaagagc cctacggctc tccgctgtga cagacttgca
    16681 gccacacacc aaaccgccac aaagacggac cttccgactc tgaaaagatt gtgtggctga
    16741 acagaggcaa ggacatccga agaagaaaaa cgatggggag gaaacgtacg cggagtgtac
    16801 gtgcacctgc tgaacgcagg ccaagttctt gacgagaaca gcagctggct agggaatatc
    16861 ctcgagcaga ccggattgaa tcggaggcag aggtgcggtc tcacgcggca ctggaggaaa
    16921 caccacggga gaacccgact gattggcgcc ttgaatgagt cccaccgaca attgcggccc
    16981 cagcgtcatg acggcgccgc tccatgcctg accggtcgtg ggattccgga aattgtacga
    17041 ttcgaagtta ttgccgaaat tgtagatgaa tccctgcgtc ccctggttat caatgtagag
    17101 attccctgga cccacgagac caaagagcgg cgccacacct ccattaaatc cgcgcacacc
    17161 tgataccgat tgcgcggccg ccggcgaaac ccagagcagt gcggctgtta cagcaaccag
    17221 catgatcccg gtcgagcgca tggtcgccct gcccttgcgc gccagcatgg acactgatcg
    17281 ccgtttgtga taccacgcca ccatcatgat gaattcagta tggctcaacc tgcatctccc
    17341 atcaaggagt ttgttatgac gaatccaatg ctccggggct gtctgctcat cggcggcgcg
    17401 acggcgctgt tgctctggac ggcgtccacc tcattcggtc tggaagtgac caaacccgtc
    17461 ccgaccgaac gacgcgaagc catgtcgttg gccgacgctg tcctcaaagc actgcagaat
    17521 aacctcgata tccatatcgg ccggcaaacc aaagaaagcc gcttggccga cattatcatt
    17581 gaacaggcaa aattcgatcc gaccatcagc ttgaacggtc aatacaacag acaggtcgcg
    17641 ccgctgaatc gacccatcct cggatttacc ggcgccaacc tgcaggacat caccaaattc
    17701 gaccagaaca cctccagcgt caccgccgac atcacccaga atctcccgac cggcgcaaat
    17761 tacgacctga actatagccc ccaacgtagc tatgtcagcg ggcccaacac cttcctgttc
    17821 aacccggcct ggaccggggg tctcgcgtta acggttacac agccgctgct gagaaacttc
    17881 ggcacggatg tcaaccggac gttcatttcg atcgcacaaa acaatgccac cgtcgaacaa
    17941 catgtgtttc tcgatcgcgt gctcactgtc attgctaccg tcgagcagac attctgggag
    18001 atggtctttg cgaatgaaaa tctgaaagtc gcgcaagcgg cactcaaggc agcggaagaa
    18061 ctcctggcca gcaaccgcgc caaagccaag gccggcgtca tgtcgatcgt cgacgtcctg
    18121 caggcggagg cggccgtcgc ttcgcgtgtc gaacagatcc tcgtggccga aaaatccatt
    18181 cgcgatcagg aagaccagct gcgccgcctc ttaaatccag cggaggaaga actgcggcag
    18241 gatctgcgcc ttatgccgac cgatccaccg accacctcgc tggaagcgat cagtcttcag
    18301 gaagctatcg atatcgccat ggaacgccgc ccggaagttt tgcaggccgg caagaacgtg
    18361 gagagcagcg acctcaatgt gaagtttgcc aagaatcaat tgctccccac cctgtcggtg
    18421 caagggacga tgggactgtc cggacttgga gccgactacg gggacgccac gagacggaat
    18481 ttcggcggag acttttataa ttacggggcg ggattagtcc tcagctaccc gatcggcaac
    18541 cgctcggcct atagcaccta caataaacgg caattggagt cccggaacgc tcaggcctcc
    18601 ttacagagcg ttcgacaaca agtgatcgtc ggtgtccggg aagccgtccg ccgcgtacac
    18661 accgatttca aacgaatcga aaccacccgc tcggctcgta tcatggccga gaagcagttg
    18721 caggccgaac aagaacgctt gaaggtcggt ctcagtacga cgcgattcgt gctcgacttt
    18781 caacgtgacc tggccacggc gcaaggcaac gagctgcgag ccacggtgga ctacaacaaa
    18841 tccttgtcga acctggccag gaataaggcc accacactcg aacggtatag tctacgactt
    18901 gaataactgc tcatgatcag gcctgaggag gcgtcggccg cgtatccgat tcagcacaat
    18961 gaacgggggg cggccctcgc gctgcttccc ctcattgcta cactcctcta ttacgcgtcc
    19021 cctcgtgaac ttcggcagga gcccctgtat cagttcctcc ctcagctttg cgcctatgcc
    19081 gcgctgttga cctggagccg cctgaacggc gcaacggtta cgaggctcgg gctcacgcgc
    19141 gccctcatcc caaggggact ccgcttgggc atcgcaacgg ggctcgccct cggcactgtt
    19201 aatctcctgg tgatcctcta tctcgtgccc tggcttgggg cagactatcg atttctcgcc
    19261 ggcacgccgc acgccaaaat tccgctgctc ctcatgctgc cctggttcat cctcatgatc
    19321 gccgccatgg tcgaactgaa ctttcgcggt tttctgttag gccggctgct gggtctggga
    19381 ttgccctccg ccctcgccgt accattgagc gccctgctgt ttgcgttcga tccctttctg
    19441 gtggcaacct ttcatcatct ccattggatt gccgtctggg atggcctgat ctggggaagt
    19501 ctgtgggtcg ccttcagaaa tctgtatgtc acgatcgtcg cccatgctgt ggaggttatc
    19561 gtcctctata gcgtcatgcg aaccatattg acctgacctt gcttgaagtt ccggtaacgg
    19621 aacttcaagc aacagttgca ctcacgacgg actcactatt cgtggctctg tgatgcggca
    19681 ccc
//
LOCUS       DAOQDS010000070        19252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1113_length_19252_cov_22.481481, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000070 DAOQDS010000000
VERSION     DAOQDS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19252
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1113_length_19252_cov_22.481481"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PK589_14915"
     CDS             <1..261
                     /locus_tag="PK589_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20952.1"
                     /translation="GTFEVLPPGAKWPRFRRVTVTYGEPLDFSADAAQLEGKVFYQHV
                     SRTVMAKIAELGQVPNPGEGQAHAGLTHDREATPTAKSCNAE"
     gene            316..2031
                     /locus_tag="PK589_14920"
     CDS             316..2031
                     /locus_tag="PK589_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNJ20953.1"
                     /translation="MSTATPQSEPKLDHDALAAMYEETFRNFEEGTITEGMVVAIGKD
                     KVVVDIGYKSEGMIPADQFSQEELGQLKVGDRLQVYLEECEDADGNLVLSKEKADKMK
                     IWEELEKLHKEEKSIEGKIISRIKGGMMVDIGVKAFLPGSQIDLHPVRDLDGLVGKTF
                     PLKIIKINHRRGNVVVSRRVLLEETRDRRRQTTLSTLKEGQLIQGTVKNITDYGAFID
                     LGGIDGLLHITDMSWGRVGHPSELFQVSDKVEVTVLKYDRETGRISLGLKQKSADPWT
                     GVAAKYPVGTRVRGRVVSLTDYGAFVELEPGVEGLVHVSEMSWTHEVRHPSRVVSVGD
                     QVEAAVLNIDPGSRKISLGMKQTAPNPWDMIEAKYPAGTRIEGKVKSLTDFGAFVGLE
                     EGIDGLIHISDMSWTKHIKHPSELFKKGQKVDAVVIRIDKEKERLSLGYKQLSRDPWE
                     EQIPNKYRVGDSITGKVSKIADFGLFIELDGDVEGLIHISEVGLDANVRMEEKFKPSD
                     DVTAKIIKVDRDERKIALSLRDHQLDSDRRQVDEFHASQGGIDQTLGRAAKQSRKRNQ
                     SDSEA"
     gene            2047..2946
                     /gene="sppA"
                     /locus_tag="PK589_14925"
     CDS             2047..2946
                     /gene="sppA"
                     /locus_tag="PK589_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNJ20954.1"
                     /translation="MGPQADTAGKPQRSLLRRILWAIVIVGGALILLNALLPDLDFST
                     QDRVALIRIEGVILDAQATISELKQYSENPLVKAIVLRIDSPGGGVVPSQEIHDAVKR
                     VKNKSNKAVIASMGTVAASGGYYIAAATDRIIANPGSLTGSIGVIMEMANFEGLMKKV
                     GVEGVVIKSGRFKDVGSPLRKMSDEERKLLQSVMDDVHQQFIQAVADGRSLEVSDVEP
                     LADGRIYTGRQAKEARLIDELGDLDDAIHIAADIAGMEGEPKVVEPRKRFSFREIIES
                     RWSSVFPKLELNTGVKLKYLMAF"
     gene            3022..3300
                     /locus_tag="PK589_14930"
     CDS             3022..3300
                     /locus_tag="PK589_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNJ20955.1"
                     /translation="MTKAQIIERVSEQVTTLTKRQAEIVVNTIFDCIRDSLRSGNKTE
                     IRGFGSFRLRARRMKEGRNPKTGATVAVPAKRVPFFKAGKELKELLNK"
     gene            3347..4309
                     /locus_tag="PK589_14935"
     CDS             3347..4309
                     /locus_tag="PK589_14935"
                     /inference="COORDINATES: protein motif:HMM:NF019968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HNJ20956.1"
                     /translation="MRWSDGALKRMERAPMFLRGMVRRLAEKKARELGYAEITEEILE
                     QFKGQMMGTMGGEGGMAEAADQMAKGQLPWTAAAKERLAAVPEFMRGMIKQIADEIAR
                     KGGHMEVNVDLFEKVEALGEIHERETTPLEWTEGALALLQQKIKESPPIAMDFVSDMI
                     RHDTEEMAREKGLTRIDEQTAVQLWEAPQERVAWSDEAWKRLQTSPDFVRSGIRKAAE
                     RRARKLGLKEVDSEHLTTFRNQAMMKAVKRIRSFGYNELTFDAFDTALQKTKRLQGND
                     QAEKRLQEIRSHFSDPEVKKPEGGTLGADLMGRFRKFLKGEGSL"
     gene            complement(4641..4967)
                     /locus_tag="PK589_14940"
     CDS             complement(4641..4967)
                     /locus_tag="PK589_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20957.1"
                     /translation="MSKKVTIPLSMYGVAEVLKWCIDRNNGRVPGVDTEGFKRMQSLL
                     AEKPTSNDYLALDQFWKKQVALELTEDEVSTVDRCLYDIPNFDNEPLPQIRHKFWPQA
                     AGIAAH"
     gene            5216..6409
                     /locus_tag="PK589_14945"
     CDS             5216..6409
                     /locus_tag="PK589_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNJ20958.1"
                     /translation="MTINTYANKRRAIGRVGLALIAGLPLLFACSKTDPYVPPDPFYY
                     FESYAVGKNPTTVTTADFNRDQITDLITTNISSNSISLLFGNGDGTFRDQVQVKVCQE
                     PRSLALNDFNHDDQLDVALACSGGDQISILFGRGNGKFDEGPQYPVHRAPVSISSQDV
                     NGDGHPDLAVALRNDKVKIFLGSARGEFRPGVQYEYGDTPTSVALKDLNQDGQPDLVV
                     TNGGPMLSAVSVWIGNGDGTFRDPKDYKTGRRPLGVSFADFNNDHLPDLLVINGEGDS
                     FTTFLGNGNGTFQPGKDSGADASPNFGVAHDFDGDHIDDVAIVNLQSTDLSILFGRGD
                     GTFHYPPRNYRTKNGPFAVASFQVSADNGEEPGLVTADNGAASVSVFLHHGRKGQIPP
                     NSKPE"
     gene            6578..6751
                     /locus_tag="PK589_14950"
     CDS             6578..6751
                     /locus_tag="PK589_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20959.1"
                     /translation="MGCLTTLAVLMVQGGVRDLVSGTNPMGSSGAILSFSTTIFGGGL
                     LAGCLALILNRLR"
     gene            6830..7018
                     /locus_tag="PK589_14955"
     CDS             6830..7018
                     /locus_tag="PK589_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20960.1"
                     /translation="MHCLKCKGFMMVERHYTLMNRRLYARCLNCGFWIDLADLLRFFH
                     KVIASSVRGTKVRETYTL"
     gene            7065..7994
                     /locus_tag="PK589_14960"
     CDS             7065..7994
                     /locus_tag="PK589_14960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNJ20961.1"
                     /translation="MNRSHRMFPVAVMVIPFLMGALWTGQALAIDNDVDDEVITVPYD
                     PRPVPSAKQAHHSLRWRHVPAHSILLKELKTGTTLYQFESEKRLSPASLTKIMSALVI
                     LEYGHLDDEVTVSPKAARAHKTHLRLRTGQIFRLEDLLKAMLIVSANDACLAASEHVG
                     GDEARFVDLMNAKAAALELHNTHFSNACGFDAPEHYSTAEDLAKLSEIALHHPVFKEL
                     VREEREIIMPINEHRAYVLRTTNRLLGRIPGVQGVKTGFTSKAGRCLIAKVSQDGSDL
                     LLVILNSKRRWNTATSLINYGLRLSDSRAALAR"
     gene            complement(8158..8709)
                     /locus_tag="PK589_14965"
     CDS             complement(8158..8709)
                     /locus_tag="PK589_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20962.1"
                     /translation="MGVKEAFMMQRTRTFTALMTMLGSLCLAAVVTAEPYELSKNDVT
                     DPKGVASQDVSLFGIKLGDDEAKALDGLVNEKIPGIKAEQEATFIFLLDQRKPTGPMA
                     GVRVQDGKVDLIFINNRFAFKTRGIFRNVLNSESPDDVRKILGKEDYGDENVMGAMLA
                     YDKQGFQVNYLGKDVNVEFSLPH"
     gene            complement(8874..9812)
                     /locus_tag="PK589_14970"
     CDS             complement(8874..9812)
                     /locus_tag="PK589_14970"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNJ20963.1"
                     /translation="MELRQLEYFMAVATHQNFSRAAEHVHVSQPSLSIQIGGLEKELG
                     TRLFDRLGRKVVLTQAGELFQVHAERALREVEQAEQVVHELLGAKRGRLIVGTLSTVN
                     SYLIAPLVSRFKLRFPDIHLQVHAQPSSEIVNGLLANRLDIGICLLPLTHAHVTTIPL
                     FEERLALIAPASMNIGKRRARMQDLAQLPLVLMPADYCLRKMVESECAKAGVHPQVVL
                     EMTSPEGILQAVAEGTGATILPELYVKSRLPASQLQIIDLYDPTPRHTVGLAYLTKRH
                     RGQAAEEFAALCETTMRDLQSDSTQPPRNKRKGRAA"
     gene            9993..10469
                     /locus_tag="PK589_14975"
     CDS             9993..10469
                     /locus_tag="PK589_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HNJ20964.1"
                     /translation="MVSKYGMGAMLLGALALCGGCADTGGGSQTSRAAAAAPAAKSLY
                     DRLGGKPAITAVVDQFVANVAADGRINNRFATTDIPKLKGHLVDQVCQATGGPCRYQG
                     REMKATHLGMQISSADFGALVQDLVAALDTFKVPAGEKGELLALLGSMKKDIVEVP"
     gene            complement(10528..10821)
                     /locus_tag="PK589_14980"
     CDS             complement(10528..10821)
                     /locus_tag="PK589_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20965.1"
                     /translation="MSHQSIASLWEEHSRDGWPQFSSPNEGQLMTLDTVIGGCAVFYL
                     DGQPEMDSHRLAILEDCVADLDGLLEDLSDESLGYFQRLRRLATALVDVGRQP"
     gene            complement(10860..11246)
                     /locus_tag="PK589_14985"
     CDS             complement(10860..11246)
                     /locus_tag="PK589_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20966.1"
                     /translation="MTRYILAAIAGIWMADGLALLSAPLLVIRRLQESLETAPNLLRW
                     QAIGIGLGLMLLLWSTPLPYQPLWWMTGGAMVIKSCFLTWGPTAWRTPLLSWCFTREA
                     VDYRFFGLWLCTLAVLLLHALGLLHG"
     gene            complement(11279..12535)
                     /locus_tag="PK589_14990"
     CDS             complement(11279..12535)
                     /locus_tag="PK589_14990"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNJ20967.1"
                     /translation="MTELNTHTFRLAVAQFNEAAEAMQLDTNLRERLKLPQRSLIVSV
                     PVRMDDGRVEVFTGYRVQHDTARGPSKGGIRYHPEVNLGEVAALSMWMTWKCALADLP
                     YGGAKGGVRVDPKQLSRAELQRLTRRYAAEIFPLIGPDKDVPAPDVGTDQQVMAWIMD
                     TYSQQVGYAVQGVVTGKPLSIGGSLGREEATGRGVVYVTLEALQHLKLDVAKATVAIQ
                     GFGNVGSHTARIMQQAGARVIAVSDVSGGLYNPKGLDIPDLLRRYHEHHEPLRDIKLG
                     EPLSNDALLQLDCTVLVPAALSEQITEANAAKLRCRILAEGANGPTTLEADRILTDKG
                     VFIIPDILANSGGVIVSYFEWVQDVQRFFWKAKDIQDRLQDIITSAFHRTLHFSLERR
                     TTMRMAALMSGIDKVAQAHLQRGLYP"
     gene            complement(12535..13368)
                     /gene="lipB"
                     /locus_tag="PK589_14995"
     CDS             complement(12535..13368)
                     /gene="lipB"
                     /locus_tag="PK589_14995"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNJ20968.1"
                     /translation="MSLDRLIHEGAAAPFAGHATAERVEGPRPATLVCGGTMAYDEAW
                     NLQRTCRAERAADRCHDLLLLIEHPSVYTLGRTTQDQHWPGEAQLSRETSIPVIRTER
                     GGSITYHGPGQLVGYPILRLSDYCAGPKAYVRMLEEVLITTLAESGITARRREGLPGV
                     WVGDDRPSKIASIGVRISHGITTHGFALNVCPDLEPFSHIVPCGISDCRVTSMSALMS
                     ATPDLRVVQQQLAAHFAARFQLTWTESVALEQFAAVMEPPNRQLFPSSIPAPANPGGA
                     P"
     gene            complement(13372..14448)
                     /locus_tag="PK589_15000"
     CDS             complement(13372..14448)
                     /locus_tag="PK589_15000"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNJ20969.1"
                     /translation="MVTGAHSPGAPSRRGLTSRSAWPVLFVFLCNLTLTAFGLVRQDA
                     AAAPLTELPVPAVVSKVRPSVVTVLTRGIPQGGSQHGAPSGSGSGIILDTNGYILTNN
                     HLVQGVTSVVVGLSTGRLTPGRVVARDFLLDLALIRITAPDLVPAEFSQRSTLEIGET
                     VIAIGNPLALKGGSTVTVGVISALDRSVLTPEGETLYDLLQTDAAINPGNSGGPLVDR
                     FGQVVGINVAIAPSAQAISYAIAIEAVTSHIESMMARGSVERPDLGLIPLTITPSVAA
                     SFDLESDRGILALQVDRAKAAGQAGLQSGDVVTAIDNRPLYNMGDFWHAIEHANGQMT
                     FQVATQGKAGTATIIIPRPSLPRP"
     gene            complement(14572..>15318)
                     /locus_tag="PK589_15005"
     CDS             complement(14572..>15318)
                     /locus_tag="PK589_15005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ20970.1"
                     /translation="ATLRSSRVTLETAKDELVRCNLRLVVNVAKHYTGRGLTLLDLVQ
                     EGNIGLMKAAERYQHRKGFKFSTYATWWIRQGITRSLADQSRTIRIPVHQTEASNRIL
                     RASRRLVQQLGRQPRLEEVASLMRMRPDRLHETMQAFQEPVALEHQVGDGGTELGELL
                     PDQQAVPPDAHVNRTELTREMDRILGTLTPREQTVIRLRFGIGEDQARTLEQVGQHLS
                     VTRERIRQIEAKALKKLKTPMVKEMFAALK"
     assembly_gap    15319..15418
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15419..15654)
                     /locus_tag="PK589_15010"
     CDS             complement(<15419..15654)
                     /locus_tag="PK589_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20971.1"
                     /translation="MMKHAIAIQDDTDPTVTVEVDPDADDAEASTLLDSIAQGDQPEA
                     DTEEAAPEKSLRSASSPFLLESLYFRSFGERGLL"
     gene            15940..16827
                     /locus_tag="PK589_15015"
     CDS             15940..16827
                     /locus_tag="PK589_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HNJ20972.1"
                     /translation="MSQLQNGERVHLVDKKGRQYALTLKAGDIYHFSGETIAHDELIG
                     KPDGSIVTLSKGKRFLALRPTFGEYVLKMPRGAQVLYPKDLSLIPMWADVYPGARVFE
                     AGTGSGALTMALLRAVGPTGLVVTYEARDDFARTALTNIERYMGPVSTLMPMRKNAYE
                     GIDLLEDGRPFDRLVLDLPEPWQVVPHAAKVLRSGGIYLSFVPTIPQVMQTVDALERT
                     SVFGNIETFETLLRTWSIQGRSVRPDHRMVAHSGFLTVARKVEEGWWSRPNRTVDTVD
                     TEDRADRDHQDQEQEDATA"
     gene            16824..17585
                     /locus_tag="PK589_15020"
     CDS             16824..17585
                     /locus_tag="PK589_15020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ20973.1"
                     /translation="MNRLQGKVAIITGGNAGIGEAIAKLFADEGAHVVVTGRRKEELD
                     RVVKGIGVNGGRALAVVGSVTDEAHVQDVVAQTTRTFGKVHILVNNAGIGDFGKRLHE
                     TDDATWAKVLDINLTGVFRMTRAAIPELIKAGGGSIVNISTVASLIGIRGLSAYAASK
                     GGLDALTRSVAVDYGQDNIRCNAVNPGLVDTPMAAPLMADPASLEPIMAQYAIRRPGK
                     PEEVAKMVLYLASDEAMWVTGQTFPIDGGMTISKG"
     gene            17652..18506
                     /locus_tag="PK589_15025"
     CDS             17652..18506
                     /locus_tag="PK589_15025"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNJ20974.1"
                     /translation="MDITPHTQWCGIIGNPVEHSLSPAIHNAAFQKMGLDLVYLAFRV
                     ESLGDALKGIRALGNARGFSVTIPHKVAAIPFLDEVEPTAKHIGAINTIVAEEGKLKG
                     YNTDASGALRALKEGGALLDGHRVLILGSGGAARAIAFALATGTGIAGLTILGIEDAE
                     RQKLVKDLRDKTGVPIEDGPLTLDMLRNWVPHVRTLIHCTPVGMSPKVGESCVPANLF
                     RPELTVMDIVYNPRETKLLQEAKAGGCRTISGLEMFLNQAVLQFELWTKQPAPVDIMR
                     HVLESRFV"
     gene            18573..19091
                     /locus_tag="PK589_15030"
     CDS             18573..19091
                     /locus_tag="PK589_15030"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNJ20975.1"
                     /translation="MNLVLIGYRGTGKSTVGKVLARKLGRTVVSTDAEIVKRANLPVP
                     EIVKQFGWDHFRDMESTVCRDFAARDQLIIDTGGGAILRPENVAVLQKNGVLVWLTAT
                     VETITRRIGGDTQRPSLTGTKSFTDEIRDVLTERTPKYQAAAAHVISTDGVSTAEVAE
                     RILKLIPHEPGH"
     gene            19176..19252
                     /locus_tag="PK589_15035"
     tRNA            19176..19252
                     /locus_tag="PK589_15035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19210..19212,aa:Arg,seq:tcg)
BASE COUNT         4068 a         5310 c         5917 g         3857 t
ORIGIN      
        1 gggacgttcg aagtgctgcc tcccggggcc aagtggccga gatttcgccg cgtgacggtg
       61 acctatggcg aaccgttgga tttctcggca gacgccgcgc agttggaagg caaagtgttt
      121 tatcaacatg tcagtcgtac ggtgatggcc aagattgcag agctgggaca ggtgccgaac
      181 cccggagagg ggcaggccca cgccgggctc actcacgatc gagaagccac accgacagcc
      241 aagtcttgca acgctgagta agactcggat tttttcacta tcagcacccg gcataggccg
      301 gaggacggac cgtttatgag tactgccaca ccacaaagcg aacccaagct cgaccacgat
      361 gccttagcgg cgatgtatga ggagaccttc cggaattttg aggaaggtac catcacggag
      421 ggcatggtgg tcgcgatcgg caaagacaaa gtggtcgtcg atatcggcta caagtctgag
      481 gggatgattc ccgccgacca gttctcacag gaagaattgg ggcaattgaa agtgggcgat
      541 cgccttcagg tgtacctcga agaatgtgaa gacgctgatg gcaacctggt gctctccaaa
      601 gaaaaagccg acaagatgaa aatctgggag gaattggaga agctgcacaa ggaagaaaaa
      661 agcatcgaag gtaagattat ttcgcgcatc aagggcggca tgatggtcga tatcggcgtc
      721 aaggcgttct tgcccggttc acagatcgat ctgcatcccg tccgcgacct ggatgggttg
      781 gtcggcaaga ccttccccct gaagatcatc aagatcaacc atcgccgtgg caatgtggtg
      841 gtttcccgac gggtcttgct ggaggaaacc cgcgatcgac gccgtcagac gaccctgtcc
      901 acgctgaagg aaggccagtt gattcaaggc acggtcaaga acatcaccga ttacggtgcc
      961 ttcatcgatc tgggtggaat cgacgggttg ttgcacatca ccgatatgtc ctggggtcgt
     1021 gtcggacatc cttccgaatt gttccaggtc agcgacaaag tcgaagtcac cgtgctcaag
     1081 tacgatcggg aaaccggccg tatttcgctc ggcctcaagc agaagtcggc ggatccctgg
     1141 accggcgtgg ccgcgaagta cccggtggga acgcgcgtgc gcgggcgggt cgtgagcctg
     1201 accgattacg gcgcgttcgt cgaactggag cccggcgtgg aaggattggt ccacgtgtcc
     1261 gagatgtcct ggacgcacga agtgcgacat ccctcgcgtg tggtgtcggt gggtgatcag
     1321 gtcgaagcgg ccgtcttgaa tatcgatccg ggaagccgca agatttcctt gggcatgaag
     1381 cagacggccc cgaatccctg ggacatgatc gaagccaaat atccagccgg cacgcggatc
     1441 gaaggcaagg tgaagagcct gaccgatttc ggcgccttcg ttggcttgga agaaggcatc
     1501 gacgggctga tccacatttc cgacatgtcc tggacgaagc acatcaagca tccgtcggag
     1561 ctcttcaaga agggccagaa ggtggatgcc gtcgtgattc gcatcgataa ggaaaaggaa
     1621 cgtctctcgc tggggtacaa gcaattgtcc cgcgatccgt gggaagagca gattcccaac
     1681 aaatatcggg tcggcgatag catcaccggc aaggtcagca agatcgctga tttcggcctc
     1741 tttatcgaat tggatgggga cgtggaaggc ttgatccaca tcagcgaagt gggtctggac
     1801 gccaacgtgc gcatggaaga aaagttcaag ccgagtgatg atgtcacggc gaagatcatc
     1861 aaggttgacc gcgatgaacg gaaaatcgcg ctcagccttc gcgatcatca gctggattcc
     1921 gaccgtcgcc aggtggatga gttccatgcc tcgcagggcg gaatcgatca aaccctgggt
     1981 cgcgcggcca aacaaagccg gaagcgtaac cagagcgatt ccgaagccta aacgcctgga
     2041 gactgaatgg gcccacaagc agacacggcg gggaaaccac agcggagcct actgcgccgt
     2101 atcctttggg ccatcgtgat tgtgggagga gcgttgattc tgttgaacgc gctcctcccc
     2161 gacctcgatt tctccaccca ggatcgtgtg gccctgattc gcatcgaggg ggtgatcctg
     2221 gatgcgcaag cgaccatcag cgagttaaag caatacagcg agaatccgct cgtgaaggcg
     2281 atcgtgttgc ggatcgacag ccccggcggg ggtgtcgtac cgtctcagga aattcacgac
     2341 gccgtaaagc gggtgaaaaa caaaagcaac aaggccgtca tcgcctccat gggaacggtt
     2401 gccgcgtcag gcggatatta cattgcggca gccaccgacc ggatcattgc caatcccggc
     2461 agcctgacgg gaagtatcgg cgtcattatg gaaatggcga acttcgaagg cctgatgaaa
     2521 aaagtcgggg tcgaaggcgt cgtcatcaag agcgggcgtt ttaaagatgt cggatcgcct
     2581 ttacgcaaaa tgagcgatga agagcgtaag ctgctgcagt ccgtgatgga cgacgtgcat
     2641 cagcaattta ttcaggccgt ggcagacgga cggtcgctgg aagtgtccga cgtagagcca
     2701 ttggccgatg gccggattta taccgggcgg caggcgaaag aggcgcggct gatcgacgaa
     2761 ttgggcgatc tcgacgacgc catccatatt gcggcggaca tcgccggcat ggaaggcgag
     2821 ccgaaggtcg tcgagccacg caaacgattt tcattccggg agatcatcga atcccggtgg
     2881 tcgtcggtgt ttcccaagct ggagttgaat accggcgtga aactgaaata cctgatggcg
     2941 ttctgagcgc ctcgaagcat gatcggcatg gcgacgcagc aattcacctg taggtaagcc
     3001 ccacccacga ggagagggac gatgaccaaa gcgcagatta tcgagcgggt atcggagcag
     3061 gtcaccacgt tgacgaagcg gcaggcggaa attgtggtca atacgatttt cgattgcatt
     3121 cgggattccc tgcgtagcgg caacaagacg gagattcgag ggttcggcag cttccgtctt
     3181 cgtgcccggc gcatgaaaga agggcgcaac ccgaagaccg gcgcgacagt tgcggtgccg
     3241 gctaaacgtg tgccattctt caaggcaggc aaggaattga aggaattact caataagtaa
     3301 caggagtgat gccgtggcag agtcgtccaa tataacagcc cctgaggtgc ggtggagtga
     3361 cggtgcgctt aaacgcatgg agcgcgcacc catgtttttg cggggaatgg tccgccgcct
     3421 ggccgagaag aaggcgcgtg agttgggcta tgccgaaatc accgaagaga tcctcgagca
     3481 gttcaaaggg cagatgatgg ggaccatggg tggggaaggc ggcatggcag aggcggccga
     3541 tcaaatggcc aaaggccagc tgccctggac cgccgccgcc aaggagcgcc tcgctgcggt
     3601 ccccgagttt atgcgaggga tgatcaagca gatcgccgat gagattgctc gcaaaggcgg
     3661 ccacatggag gtcaatgtcg acctgttcga aaaggtcgaa gccttgggag agatccacga
     3721 gcgcgaaacg actccgttgg aatggaccga aggcgcatta gcactcctgc agcagaagat
     3781 caaagagtct ccgccgatcg ccatggactt cgtgagtgac atgatccggc atgatactga
     3841 ggagatggcg cgggagaagg ggttgaccag aatcgacgag cagaccgccg tgcagttgtg
     3901 ggaagcgcct caggagcgcg tggcctggag cgacgaagcc tggaagcgtc tgcagacctc
     3961 gcccgatttt gtgcgcagcg gcattcgtaa agcggccgaa cgcagggccc gcaagttggg
     4021 tttgaaagaa gtcgactcag agcatctcac gacgtttcgc aaccaggcca tgatgaaagc
     4081 cgtgaagcga atccggtcct tcggatataa cgagctgacg tttgatgcct ttgacaccgc
     4141 acttcaaaag accaagcggt tgcaaggcaa cgatcaagcg gaaaagcggc tgcaggaaat
     4201 tcgttcccac ttcagtgatc ccgaagtgaa aaagccggaa ggcggcactt tgggggccga
     4261 cctcatggga cgattcagaa aattcctcaa gggcgaaggg tctctgtaag aatcgacggc
     4321 gtcgccatga tgatgcctgg cggcgctgtt ctcatctctt tctccacgtc gctctcactt
     4381 ccactgttgt gcttccatct tgtgtctggg gtgcccccac gcacatggca cggcgttgca
     4441 catctcctgc atttcttttg agctctcggc gatggcaaga tctgacagat cgtctgaagc
     4501 ggctgaaatt gaaacagcct tctcagggat gatcaaactg gtcgacgctg gtgcgagagc
     4561 agacgtgaaa agtgatatca cccacaagcc aagggcctgt gggtgatatc ggacattgag
     4621 ggaagtatcg aggctgtcga ttagtgagcc gcgatgccgg ctgcttgggg ccagaattta
     4681 tgccggatct ggggcagcgg ttcgttatcg aagttcggaa tgtcgtagag gcaacggtca
     4741 acggtcgaaa cttcgtcttc cgtcaattcc agtgcgactt gtttcttcca gaattgatcg
     4801 agcgccagat agtcgttgga cgtcggcttc tcggccaaca gggactgcat ccgcttgaac
     4861 ccttctgtat cgactcccgg cacacggccg ttattgcgat caatacacca tttcaggact
     4921 tctgcgacgc catacatcga caacggaatt gtgactttct tgctcatggg cacctcctcc
     4981 acacttgggc cgcattgtag cctaaggctt tccaagtttt caaaggggcg aaaggcctag
     5041 acgtccgagt ccattcgggg ggagcacaac gaccaagccc cacaccgcaa ccctgacgcg
     5101 gggacttcgt tgcgaagcag aagggggctc tgtatactcc gctcagcgcg gcaggattgg
     5161 gagcggcgaa gcatggactt ggccggaatg tcatgctttt ttcatcggga tggcgatgac
     5221 catcaatacc tatgccaaca agaggcgggc gatcggtcga gtggggttgg cactcatagc
     5281 cggcttgccg ctcctcttcg cctgctccaa aacggatccg tacgtcccgc ccgatccatt
     5341 ttattacttt gaaagctatg ccgtcggcaa aaacccgacg accgtgacga cggccgactt
     5401 caatcgggat caaatcaccg acctcatcac gaccaacatc tcgagcaatt cgatttccct
     5461 tctgttcggc aacggcgacg gaacctttcg ggaccaggtt caggtcaagg tatgccagga
     5521 gccacggtcc ctcgccctca acgacttcaa ccatgatgac caactcgacg tcgcgctggc
     5581 ttgttcagga ggggatcaga tctccatcct gttcgggcga ggcaacggaa agtttgatga
     5641 aggtccccag tacccggtcc atcgcgcccc ggtgtccatc tccagccagg acgtcaacgg
     5701 cgatgggcat ccggatttag cggtggcttt acgcaacgac aaggtgaaga tttttctcgg
     5761 gagcgcccgt ggcgaattcc ggcccggcgt gcaatacgaa tatggcgata ctcccacctc
     5821 cgtggcgctg aaagatctca atcaagatgg ccagccggat ttggtcgtca cgaacggtgg
     5881 gccgatgttg agcgccgtct ctgtatggat tggcaatggc gatgggacgt tccgcgatcc
     5941 caaagactac aaaaccggtc ggcggccgtt gggggtcagt ttcgctgact ttaacaacga
     6001 ccacctacct gatctgctgg tcatcaacgg ggagggggat agttttacca ccttcctcgg
     6061 caacgggaat gggacctttc agccggggaa ggattccggc gcggatgcca gccccaattt
     6121 cggcgtggcc catgacttcg atggcgacca tatcgacgat gtagcgatcg tcaatttgca
     6181 gtccacggac ctgtctattt tgttcggccg tggcgacggg acattccatt acccgccgcg
     6241 taactaccgc acgaaaaacg ggccgtttgc cgtggccagt tttcaggtct ctgccgacaa
     6301 cggagaggag ccggggcttg tgacggccga taacggggcg gccagtgttt cggtgtttct
     6361 gcatcacggc cggaaagggc agatcccgcc aaacagtaaa ccggaatagc ggccgattca
     6421 gatttgagcc gcgacaaccg aagaagagac aggcagtttg cttgacacct cgatagggct
     6481 cggatagagt tcgaaaaagt cgctggacac aggtcctgcg ccattttcag cgatcagacc
     6541 gcgggggctc gtgcgatcgt ttttgtggtg gtttagcatg ggctgcctga cgacgctggc
     6601 agtcttgatg gtccaaggcg gggtacgcga tctggtgtcg gggaccaacc cgatgggaag
     6661 ttccggcgcg atcctgtcct tcagtaccac gatcttcggg ggtgggttgt tggccggctg
     6721 cctcgccctt atcctcaatc gtctccgcta aacaaatgac gacgttggta tcggtctgag
     6781 atgcgccgag acgtctacgc aatcatgtcg aaggggcaat ggtcgggcga tgcactgcct
     6841 gaaatgcaaa ggattcatga tggtggagcg gcattacacg ctcatgaatc gccgactgta
     6901 tgcacgctgc ctgaattgcg ggttctggat cgatctggcc gacctgctcc ggttttttca
     6961 caaagtcatc gccagcagtg tacgtggcac gaaggttcga gaaacctata ccctgtgacc
     7021 gccagaatcc ctctgctcgt cacaagaaag catagatatt ccgcatgaac cgttcacatc
     7081 ggatgttccc ggtggccgtc atggtcattc cgttcctcat gggcgccctt tggactggtc
     7141 aggccctggc gatcgacaac gatgtggatg acgaggtcat taccgttccc tacgatccca
     7201 ggccggtgcc ctcggcgaaa caagcgcatc acagccttcg gtggcggcac gtgccggcgc
     7261 atagcattct cctgaaggaa ttgaagacgg gcacgacgct ctaccagttt gaaagcgaaa
     7321 agcggttgtc cccggcgagc ctcaccaaga tcatgtctgc gttagtgatc ctggaatatg
     7381 gccatctgga tgatgaagtg accgtcagcc ctaaggccgc ccgcgcccat aaaacgcacc
     7441 tgcggctgcg cacgggacaa atattccgac tggaagattt actaaaggcg atgctgattg
     7501 tttccgcgaa cgatgcctgc ctcgcggcca gcgaacacgt cggcggcgat gaagcccggt
     7561 ttgtcgatct catgaatgcc aaggccgcgg cgctcgaact gcacaatacc catttcagca
     7621 atgcctgcgg ctttgatgcg ccggaacatt attcgaccgc cgaagatctc gccaagctca
     7681 gcgagattgc gttgcaccat cccgtattca aggaactggt ccgcgaagag cgggaaatca
     7741 tcatgccgat caacgagcat cgcgcctatg tcttacgcac caccaatcgg ctccttggcc
     7801 ggattcccgg ggtgcaaggg gtaaagacgg ggtttacctc gaaggccggt cggtgcctga
     7861 tcgcgaaagt ctcccaggat ggcagtgatc tgctgctggt gatcctcaac tccaagcgtc
     7921 gctggaatac ggcgaccagc ctcatcaatt acggcttgcg tctgagcgat agccgcgccg
     7981 ccctggcccg ataaatttcg ccattgccaa tggttgcacg gaatccgtgc tgatctcgcc
     8041 ttcagcgagg ctcgcacgag tgcgtcctgt gatgcgcagg gcgtgatggc ggactgatcg
     8101 gatagttaaa ggccaggaac ctgcgagggg ctgagcggga atcgcctctg tgacggttta
     8161 gtgcgggagt gaaaactcca cgttgacgtc cttgccgaga taattcacct gaaagccctg
     8221 cttgtcatag gcgagcatcg cgcccatcac attctcgtcg ccataatctt ccttgcccag
     8281 gatcttgcgc acatcgtcgg ggctttcgct gttgaggacg ttgcggaaga tgccgcgtgt
     8341 cttaaacgca aaacgattgt tgatgaagat gagatcaact ttgccgtctt gcacccgcac
     8401 gccggccatt ggcccggtcg gtttgcgctg atcgagcagg aaaataaagg tggcttcttg
     8461 ttccgccttg atgccgggga ttttttcgtt caccagcccg tcgagagcct tcgcttcatc
     8521 atcccccagc ttgattccga acagtgacac atcctggctg gcgactcctt tcgggtcagt
     8581 tacgtcgttc ttgctcagtt cataaggttc ggcagtcacg acggcagcga ggcacaggga
     8641 ccccagcata gtcattaagg ctgtgaatgt ccgtgtacgc tgcatcatga atgcctcctt
     8701 aacgcccatg ctgaaaattt gtccgggtcg ccactaccgg tcacataaat gaggagaggg
     8761 tcaggcaaca gggcattcta tccaagcggc tgtctgactt tcaaggtcgc cgcaggctta
     8821 ttgcgagctg cagacgcgcg gattcgggct ctgcaggaag gcggtttgag atcttaggcg
     8881 gctcttcctt tgcgcttgtt gcggggaggt tgtgtggagt ctgactgtag atcacgcatc
     8941 gtagtttcac acagggctgc gaattcttcg gccgcttgtc cccgatgccg tttggtgagg
     9001 taggcgagtc cgacggtatg acggggcgtg ggatcgtaca gatcgatgat ctggagttgt
     9061 gacgccggca gccgggattt gacgtagagc tcgggaagga tcgttgcccc ggttccttcc
     9121 gctaccgcct gaagaatgcc ttccggggaa gtcatttcca aaaccacctg cgggtgcaca
     9181 cctgccttcg cacattccga ttcgaccatt ttccgcagac aataatctgc cggcatcaag
     9241 accaacggca attgtgcgag atcctgcatg cgtgcacggc gttttccgat gttcatactg
     9301 gcgggggcga tgagcgcgag acgttcctca aacagcggaa tggtggtgac gtgcgcatga
     9361 gtcaggggga gtagacaaat cccgatatca agccgattcg ccagcaatcc gttgacgatc
     9421 tcggaagacg gttgggcatg cacctgcaga tgaatgtcgg gaaagcgtag tttgaatcgg
     9481 gagaccagcg gcgcaatcag ataggaattg accgtcgata gcgtgccgac gatcaggcga
     9541 ccgcgtttcg ccccgagcag ttcgtgcacg acctgttccg cttgctccac ctcccgcaag
     9601 gcccgctctg catgtacctg aaagagttca cctgcctgcg tcaggaccac ctttctcccg
     9661 aggcggtcga acagacgcgt gcccaactcc ttttcgagcc cgccgatctg aatcgacaac
     9721 gaaggctgcg agacatgcac atgctccgcg gcgcggctga aattctggtg cgtcgcgacg
     9781 gccatgaaat attcaagttg ccgcagttcc atgagagttc agaccagcta tagccaaaga
     9841 ctatggaaag tatgaaaaca atatattaga cgaatagatc atagatctcc taaggtgaat
     9901 cgtcaaccgg aatgtcgctt cacaaagcgc ttcgcatgtc ctgtcggcgg cgaggtcagg
     9961 caaccggcca ttcatcatca aggaggtggg cgatggtatc gaaatacggg atgggggcaa
    10021 tgctcttggg ggccctggcg ttgtgtggcg gatgcgccga caccgggggg ggcagccaga
    10081 cttcgcgcgc ggcggcggcc gctcctgcgg caaagtcgtt gtacgaccga ttgggcggca
    10141 agccggcgat tacggcggtg gtcgaccagt ttgtggcgaa tgtcgcggcg gatggacgga
    10201 tcaacaaccg gtttgcgacc accgacatcc ctaagttgaa ggggcatctc gttgatcagg
    10261 tttgccaggc aacaggcgga ccttgccgat atcaggggcg cgagatgaag gcgacgcact
    10321 tgggcatgca aatcagctca gcggatttcg gggcgttggt tcaggatctg gtggcggcgc
    10381 tcgacacgtt caaggtgcct gctggggaaa agggcgagct tctggcatta ctcggttcga
    10441 tgaagaagga catcgtcgag gtgccgtaaa tgagcgacgg cgggggtcgg cctgagggcc
    10501 ggtcctcgcc cgagatcggg cacagagtta gggctgccgt cccacgtcga cgagagcggt
    10561 cgccagccgg cgaaggcgct ggaaatagcc caggctctca tccgacaagt cttcaagaag
    10621 gccatcgaga tcggccacac aatcctccag gattgcaagg cgatggctat ccatttccgg
    10681 ttgcccgtcg agataaaaca ccgcgcatcc gccgatcacg gtatccagcg tcatcaactg
    10741 gccttcattc gggctggaaa attgaggcca gccgtcgcgg ctgtgttctt cccagagaga
    10801 ggcaatggat tggtggctca taggatggta ccggtccaag gtgaagctgc ggcgacgcgt
    10861 tacccgtgca gcaaccctaa cgcatgcaag agcaggacgg caagcgtgca aagccagaga
    10921 ccgaagaatc gatagtccac cgcctcgcgc gtgaagcacc aactcaacag cggtgttcgc
    10981 caggcggtgg ggccccaggt caaaaagcag cttttgatga ccatggcacc accggtcatc
    11041 caccagagtg gttgataggg cagcggggtc gaccagagca ggagcatgag cccgagaccg
    11101 attccaatcg cttgccagcg gagcaggttc ggagccgtct cgagggattc ctgcagccgc
    11161 cggatcacga gcagaggggc actcagcagg gcgagtccgt cggccatcca gatgccggca
    11221 atcgccgcaa ggatgtatcg ggtcatactg tgaatcgcag gttcagcata gacagggttt
    11281 acgggtagag cccgcgttgg agatgggcct gggctacttt gtcgatgccc gacatgaggg
    11341 ccgccatccg catcgttgtt cgccgttcga gcgaaaagtg cagggttcta tggaaggcgc
    11401 tggtgatgat gtcttgtaac cggtcttgaa tgtcctttgc tttccagaag aagcgctgca
    11461 cgtcctgcac ccattcaaaa tacgacacga tcacgccgcc cgagttggcc agaatatcgg
    11521 gaatgatgaa gaccccctta tcggtgagga tgcgatcggc ctccagtgtc gtggggccgt
    11581 ttgcgccttc tgccagaatg cggcaacgta acttcgccgc attggcttcg gtgatttgtt
    11641 cggacagggc ggcaggaacc agcacggtac agtccagttg gagcaatgcg tcattgctga
    11701 gcggttcgcc caacttgatg tcgcgtagcg gctcgtggtg ttcgtggtac cgacgcaaga
    11761 ggtccggaat gtcgaggccc ttggggttat agagcccgcc gctcacgtcg ctcacggcaa
    11821 tcacccgtgc gccggcctgt tgcatgatgc gcgcggtatg ggagccgacg ttgccgaacc
    11881 cctggatggc gaccgtcgct tttgcaacgt cgagtttcag gtgttggagc gcttccaggg
    11941 tcacatagac cacaccacga ccggtggctt cttctcgtcc gagactgcct ccgatggaca
    12001 acggctttcc ggtgacgacg ccttgcaccg catagccgac ttgttggcta taggtgtcca
    12061 tgatccaggc catcacctgc tgatcggtcc cgacatcagg cgcaggcacg tccttatccg
    12121 gtccgatgag ggggaaaatc tctgcggcgt agcgccgggt gaggcgttgg agttccgcac
    12181 ggctcagttg tttgggatct actctcacgc cgcctttcgc cccgccgtag ggcaggtcgg
    12241 ccaaggcgca tttccaggtc atccacatgg aaagggccgc gacttcgccc aagtttacct
    12301 ccggatggta ccggatgccg cctttcgacg ggccgcgagc cgtatcgtgc tggacccggt
    12361 atcccgtgaa gacttcaacg cggccatcgt ccatccggac cggcacgctg acaatcaacg
    12421 agcgttgcgg aagtttcagc cgctcgcgca ggttggtatc cagctgcatt gcttcggccg
    12481 cttcattgaa ttgggcgacg gccaaacgga aggtgtgggt attcagttca gtcatcaggg
    12541 cgctcctcca gggttggctg gcgcaggaat ggatgaaggg aacagttgac ggttcggagg
    12601 ttccatcaca gccgcgaact gttcaagcgc cacggactcc gtccacgtga gctgaaaccg
    12661 tgcggcaaag tgcgcggcaa gctgttgctg cacgacacgg agatcgggcg tggcgctcat
    12721 gagggccgac atggatgtga cccggcaatc ggaaatgccg caaggcacga tgtgagaaaa
    12781 aggctcaagg tcagggcaga cattcaaggc gaagccgtgc gtggtaatgc cgtgggagat
    12841 acgcacgccg attgacgcga ttttagaggg gcggtcgtcg ccgacccaga cccccggcag
    12901 gccttcacgt cgacgagcgg tgatacccga ctcggcgagc gtggtaatca acacctcttc
    12961 aagcatgcga acgtaggctt tcggccccgc acagtaatca ctcagtcgga ggatgggata
    13021 gccgaccagc tgtcctggcc cgtggtacgt aatcgagccg ccgcgctctg tccggatgac
    13081 ggggatgctg gtctcccggg acagctgcgc ttctcccggc caatgctggt cctgcgtggt
    13141 gcggcccagg gtatacacgg aagggtgttc gatcaggagc agcaggtcgt gacagcggtc
    13201 tgccgctcgt tcggcccgac aggtgcgttg caggttccac gcttcgtcat acgccatcgt
    13261 cccgccgcat actaacgtcg cgggtcgtgg tccctcgacg cgttcggcgg tcgcatggcc
    13321 tgcgaacggt gcggcagctc cttcatggat gagacgatcg aggctcatgg cttacggccg
    13381 tggcaggctg gggcgtggaa tgatgatcgt agccgttccg gctttgcctt gtgtcgcgac
    13441 ctggaacgtc atctgcccgt tggcgtgctc gatggcatgc cagaagtctc ccatattgta
    13501 gagcggacgg ttatcaatgg ccgtcaccac atccccggac tgcaatccgg cttggccggc
    13561 tgctttcgcg cggtccacct gcagggcgag gatgccgcgg tcgctttcca gatcaaagct
    13621 tgcggccaca ctgggcgtga tggtcagggg aattagtccg aggtcaggcc gttcaacgga
    13681 tccacgggcc atcattgatt cgatatgcga ggtcacagcc tcgatggcga tagcataact
    13741 gatggcttgg gccgacgggg caatagcgac attgatgccg acgacctggc cgaagcgatc
    13801 gaccagcggt ccaccgctat tccccggatt gatggcggca tccgtttgca gcaaatcgta
    13861 gagggtctct ccttccggtg tcagaacaga gcggtccagc gcactgatca caccgacggt
    13921 cacggtggag cctcccttca acgccagcgg gttgccgatg gcgatgaccg tttctccaat
    13981 ctccagggtg gagcgttggc tgaattcggc agggacgaga tcgggcgcgg tgatcctgat
    14041 gagggcgagg tccaggagaa aatcacgggc gacgacacgt ccgggggtca gccggcccgt
    14101 agataatcca accacgacac tggtcacgcc ttgaacgagg tgattattgg tcaggatata
    14161 gccgttggtg tcgagaatga tcccggaacc ggatcccgaa ggcgcgccat gttgcgaacc
    14221 gccctgcggg atgccgcgtg tcagtacggt cactaccgag ggccgtactt tcgacacgac
    14281 ggcgggtacc gggagttcgg tcagcggggc ggcagcggcg tcctggcgca ctagcccgaa
    14341 tgccgtcagc gtgagattac acagaaagac gaagagaacg ggccaggcag atcgagaagt
    14401 gagaccgcgt ctggatggtg caccgggact gtgggcgcct gtcaccatgg aaagctgatc
    14461 ctccgccgcc gcattctctc acactcgccc gtgcggcgaa agcgcccgga aaacaaaacg
    14521 ggcgaggccg aggcctcgcc cgtctcgcgc tcccgtggcg tcgtaccgtc gctacttcag
    14581 cgcggcaaac atctccttga ccatgggggt cttgagcttc ttcaacgctt tggcctcgat
    14641 ctgtcgaatc cgttcacgcg tgaccgacag atgttggccc acctgctcga gcgtccgtgc
    14701 ttgatcttcg ccgatgccga atcgcaaccg gatgaccgtt tgctctctgg gtgtgagtgt
    14761 cccgagaatg cggtccatct cgcgggtgag ttccgtgcga ttcacgtggg catcgggcgg
    14821 gaccgcctgc tggtccggca gaagttcccc gagttccgtt ccgccatcgc cgacctgatg
    14881 ctccagtgcc accggttcct gaaacgcctg catcgtttca tgcagacgat cgggccgcat
    14941 ccgcatcagc gatgccactt cttccagccg cggctgacga cccaattgct gcacgaggcg
    15001 gcgcgacgcc cggaggatgc gattcgacgc ttctgtctga tgcaccggga ttctgatggt
    15061 gcgcgattga tcggccagag aacgtgtaat gccttgccgg atccaccacg tggcataggt
    15121 gctgaatttg aaccccttac gatgctgata gcgctcggcg gccttcatca ggccgatatt
    15181 gccttcctgc accaggtcca gcaaagtgag ccctcgcccg gtgtaatgct ttgcgacatt
    15241 gacaaccagc cgaaggttgc agcgcaccag ctcgtctttc gccgtttcca gcgtgacgcg
    15301 tgaactgcgc aaggtggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc
    15421 cagcagtccc cgctcgccga atgaacgaaa gtataaagat tccaaaagaa aaggggagga
    15481 agccgaacgc aatgattttt cgggagccgc ttcctcggtg tccgcctcag gctggtcgcc
    15541 ctgggcgatg ctgtccagca gcgtactggc ttccgcatcg tcggcgtccg gatcgacttc
    15601 cacggtcacc gttggatctg tatcgtcttg aatcgcgatc gcgtgtttca tcatgtgtca
    15661 cccctcttcc ccgtcctggg gatggttggc tacgggaacc cttgggaaag atgccctcat
    15721 ctctgtaaga gaccagaaca ggaaaaagga ttcgtgtcgt ctctcattgt ccggctgtcc
    15781 ttggtgatac tacgagagca cgaggctgtt cagatttctg tctaattgag atagggacgc
    15841 tcgctgaact gtcaagggat cggccggaca gagtcttaag cgctgattcg acgggggttc
    15901 ggttgaaaac gcgtcggcca tctcctagaa tgccgaccta tgtcacaact acaaaacgga
    15961 gaacgtgttc atctggtcga caaaaagggc cgtcagtacg ccctgacctt gaaagccggg
    16021 gatatctatc acttcagcgg ggagacgatt gcgcacgacg agttaatcgg gaagcctgac
    16081 ggatcgatcg tcaccctctc gaaaggcaaa cgcttcctcg ccttgcggcc gaccttcggc
    16141 gagtacgtcc tcaaaatgcc ccgcggggcg caggtattgt atcccaagga tctatcgttg
    16201 attcccatgt gggcggatgt gtatcccggc gcgcgggtgt tcgaagccgg aaccggttcc
    16261 ggcgcgctca cgatggcctt gttgcgcgcc gtggggccga ccgggctggt cgtgacctat
    16321 gaagcccgtg atgactttgc ccgcacggcc ctcacgaata tcgaacgcta catggggccg
    16381 gtgtccaccc tgatgcctat gcgaaagaat gcctatgagg gcattgacct tctcgaagac
    16441 ggccgcccgt tcgatcgcct tgtgttggat ttgcccgaac cctggcaggt cgtcccgcat
    16501 gcggcgaagg tcttgcgctc cggtggcatt tatttgagct tcgtgcccac catcccgcaa
    16561 gtcatgcaaa ctgttgacgc gctggaacgc acgtcggtgt ttggtaacat cgaaactttt
    16621 gagacgttgc tgcgcacctg gtcgatccag ggacgtagcg tccgccccga ccatcggatg
    16681 gtggcgcact cagggttttt gaccgtggca agaaaggtcg aggagggctg gtggtcccgt
    16741 ccgaacagga ccgtcgacac agtcgacacg gaggaccggg ctgacaggga ccatcaggac
    16801 caggagcagg aggacgcaac ggcatgaatc ggttacaagg gaaagtggcc atcattaccg
    16861 gtggcaatgc aggaatcggc gaggccattg ccaaattgtt tgcggatgaa ggggcgcacg
    16921 tcgtcgtcac cggtcgccgg aaagaggaac tcgaccgtgt cgtgaaaggt atcggggtga
    16981 acggcggacg agcgctggcc gtcgtcggct ccgtcacgga tgaagcccat gtccaggacg
    17041 tggtcgcgca gaccaccagg acgttcggga aggtgcacat tctggtaaac aatgccggca
    17101 tcggcgattt cggcaagcga cttcatgaga cggacgacgc cacctgggcg aaagtgttgg
    17161 acatcaattt gacgggcgtg ttccggatga cgcgagcggc gatcccggaa ttgatcaagg
    17221 ccggtggcgg gtcgatcgtg aatatctcta ccgtggccag tctgatcggc attcggggac
    17281 tgtcggccta tgccgcatcc aaaggcggct tggatgcatt gaccaggtcg gtggcggtgg
    17341 attacgggca ggacaatatc cgctgcaatg cggtgaaccc cgggttggtc gacacgccga
    17401 tggcggcgcc gttgatggcg gatcctgcca gtttggaacc catcatggcc caatatgcca
    17461 tccgtcggcc ggggaagccc gaggaagtcg ccaagatggt gctgtacctc gcttccgatg
    17521 aagcgatgtg ggtgaccggt cagaccttcc cgatcgatgg gggcatgacg atcagcaaag
    17581 gctaaggtag cagtctcccg tgccgggcaa agtcgggggc atcatcgttg tacgaagcag
    17641 gatcatcacg tatggacatc acacctcaca ctcaatggtg cggcatcatt ggcaaccccg
    17701 tcgagcattc gctgtcgccg gcgatccata atgccgcctt tcagaaaatg gggctggatc
    17761 tcgtctatct ggccttccgc gtggaatccc tcggtgacgc attgaaaggc atacgagcct
    17821 tggggaatgc acggggattc agcgtaacca ttccgcacaa ggtggcggcc attccgttcc
    17881 tcgatgaggt ggagcctacg gccaagcaca ttggcgccat caacaccatc gtcgcggaag
    17941 agggaaagct caaagggtac aacacggatg cgtcgggagc gttacgggca ttgaaagaag
    18001 gcggcgcgct gctcgacggc catcgagtgt taatcctggg ttcgggcgga gctgctcgcg
    18061 ccattgcgtt tgcgctggcc acgggaacag gcatcgcggg gctgactatt ctggggatcg
    18121 aagacgcgga gcggcagaag ctggtgaagg atttgcgcga taagaccgga gtccccatcg
    18181 aggacggtcc gctgacattg gacatgcttc ggaattgggt cccacatgtc cgcacgttga
    18241 tccactgcac gccggtcggg atgtccccca aggtcgggga gtcgtgcgtg ccggccaatc
    18301 tgttcagacc tgagctcacg gtgatggata tcgtctacaa cccgcgcgaa accaagttgc
    18361 ttcaggaagc gaaggccggc ggatgccgga ccatttccgg gttggagatg tttctcaatc
    18421 aagccgtgct gcaattcgag ttgtggacga aacagccggc gcctgtcgat attatgcggc
    18481 atgtattgga gtcgcggttt gtctagcgaa tcagcccctc tccgcgccaa atccgcagtg
    18541 gcgaccgcat cctttccagc ttggaaactc ctatgaatct cgtcctgatc ggatatcgcg
    18601 gaaccggtaa gagtacggtg ggcaaagttc tcgcccgcaa gctcggccgc acggtcgtct
    18661 cgacagatgc cgagatcgtc aagcgcgcaa acctccctgt gccggaaatc gtcaagcaat
    18721 tcgggtggga ccattttcgt gatatggagt cgaccgtgtg tcgggatttt gccgcacggg
    18781 atcagttgat catcgatacc ggtggcggtg ccattcttcg gccggagaat gtggcagtgc
    18841 tgcaaaagaa cggcgtgttg gtctggctta ctgcgaccgt cgaaacgatt acacgccgga
    18901 tcggtggcga tacgcaacgg ccgtcattga caggcacaaa atccttcacc gacgaaatcc
    18961 gtgacgtcct gaccgagcgg accccgaagt atcaggctgc cgccgcccat gtgatctcga
    19021 ctgacggcgt ctccacggca gaagtggccg aacgcattct caaactgatc ccgcacgagc
    19081 caggccattg agtcggagac cgctgcgact gaaatcagcc gtgcctcgtc accttccttg
    19141 acttcccctc agtaacatgg ttttaatcgc cgcgcgcgcc cgtagctcaa ttggatagag
    19201 catcggactt cggatccgag ggttgggggt tcaagtccct ccgggcgcac ca
//
LOCUS       DAOQDS010000071        18749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1165_length_18749_cov_17.541671, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000071 DAOQDS010000000
VERSION     DAOQDS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18749
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1165_length_18749_cov_17.541671"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            923..2098
                     /locus_tag="PK589_15040"
     CDS             923..2098
                     /locus_tag="PK589_15040"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNJ20976.1"
                     /translation="MTRSATPVMSLSPNLDFLRAIAVLLVYFFHLFITTGPRLPDFLA
                     QFGVILFFVHTSLVLMFSLQRIDLKGQRLFATFYLRRLFRIYPLSIVCVSVIILLQLP
                     RAPWWPWTQPDTSTILANLLLYTEFTYKPVVTSVLWSLPYEVAMYAVLPFCYLAGKAY
                     GIRGILALWGLAVVGGFVQPYISWRLGLAQFAPCFIAGVACYFMGYGARRRPLPFWGW
                     PLILLAAAGIQGFGSYYEYEQVSRWVLCLMIGLTAPFFAELNRPMLNKATELIARYSY
                     GIYLTHLHAQWAALVLLKDQPAAVRYSVLVVLSVGLPVALYHMVEAPMIRLGARLAAN
                     LPTKSVPVVRPASPQRALPAPAEAAPYGRPSQPVRPRPGQAPAVRTGKSAPAPASAR"
     gene            2306..2932
                     /locus_tag="PK589_15045"
     CDS             2306..2932
                     /locus_tag="PK589_15045"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNJ20977.1"
                     /translation="MLTRFLLCLVLAAATLPTAGLALAAERTNVEYSADQIIESEDGA
                     TKSRIYSTPTKERRDMSQGGAAMTMITRHDKHVSWTVMPEEKMYMEAPATKQGQDKSD
                     LSGYQIEQTALGEETVNGVVMNKSKIIMTGNDGTKMGGFMWTTKEGILAKIDALAVEK
                     GQKARFKMEQTNIQIGKQPAELFEIPKGFEKLDMGGMGMDMLKGMMGR"
     gene            3063..3851
                     /locus_tag="PK589_15050"
     CDS             3063..3851
                     /locus_tag="PK589_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20978.1"
                     /translation="MKKAEELERAGQPREAFSAATKANGDCVSDTQRHEGVQQRTAKA
                     IGAEEEKKARLEDAFTWYVRAKAPADAGRMQRRLVEAKPDDINTLSHAIDYFSQQNDK
                     AQEEAMRTRARKNVEQALAEEEKRFASFSRDSLHELGRARDWAYYAKTGEDRIRARAA
                     QRGDTLAAEDGRKFLRLAMSYYEQADRPDGVKKIREKARMLAKRHEAKGEGEIAAEFY
                     AMAGDDKQADTVQKQTEQRQAQAEEGRKKTFKKEQADLEKALGF"
     gene            4127..7582
                     /locus_tag="PK589_15055"
     CDS             4127..7582
                     /locus_tag="PK589_15055"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ20979.1"
                     /translation="MAKKQSGTVSWATSAEWDVRTLEPARGGRRGATEPPDPFLVNLT
                     ATKRWEVEQTLTATPKARRGETAPAPLTLDVEGDPDDVYIVMTRYASGAIRFHMPNEP
                     ARRGRRRSARTLYRFSIPVPAAPLAEPGGRRGFISAAIKAVVLKVAGKLADYALAKLA
                     LLWETATWKYKGRREGWLSVTGEALGGQQMLPAADLSSLSTTGRNLLFLHGTFSSTTA
                     AFNGLATTRGSRGKTFFDELQDLYGNRIYGFDHFTLSRTPEENVRMLLEALPDRTTTF
                     DVITHSRGGLVLRHLVERRDRFGALADRFAVGRAVLVASPNGGTPLASPDHVTQYTNW
                     LSNVLELFPDNPFTTGVEFVSEALSWIARRLVGSLPGLASMDNNGEIIQALQAAPNPP
                     GQAYSALVANFEPDAGLLQRIIDAGADLFFPTANDLVVPTEGGWRVDPGSGSVPAIPG
                     DRIGCFGVGGNLSQQQPVNHVNFFQDPGTVDFLVRALRGESQLLTPIDCNGDLPTGRR
                     RGAALQKTPPAAVPTVRREEQPTTRPTLPAVQVNPPVVAVEPYPEPDDVFHIALIAPK
                     AGADEAQLIATFRNARVMEHLHTGGSGKTTATGRRGTSDHAPTQWDRIKAGQAHIHGY
                     INGDPAYPQLPDETALQQMGSALFMALFPGQVRRLYDAARSEQVSQRLNLIFTSDIGW
                     IADQSWEFIYDPDRRTFLALEEVNFTRNVLTAIPAERIPARPTMRILVVVAQPLGLGA
                     LSVEEEADVIRSGFRRLLDAGLAEVELLLDATPELLHRALESAAAPIDVLHFIGHGEY
                     NEQTDCGYLIFENQDGGEQRLDSSTLRQIVCRRGIRLVCLNACETGRGGRQDFSRGVA
                     QALIAGGVPAVVANQYPVLDVSATAFSRHFYWALAMGHPIGDAAREARVSVNYAISGE
                     AIDWAVPVVFARNPAQRLCVPRTAAGYERSRRAVVRQRRRAVEDRIKIGIWNAHRMIP
                     HLPDICERLTKAQDRYAFETVSFPAPIGIWRREQEKDQAFVVAETLYDRLKGKPKELG
                     VETLICMINFPMKDARTRYLYYWRRDPLIVTSTFGLLEQLNEREFTVERMMAHLAAAV
                     VAGIPPHPRRVGPADCPLFYNEQRDIRLIAGRLTFCANCRKQFKDKPAQERLRAAERL
                     LAAYP"
     gene            7808..9037
                     /locus_tag="PK589_15060"
     CDS             7808..9037
                     /locus_tag="PK589_15060"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ20980.1"
                     /translation="MSTKIKTFVLCCSTLVLTACPQPHAVPPPSFPPSWETLIRKGDI
                     EQEYWRYPEAEYYYSQAVTRAQEFGADDARLARSLRSLSSVVSSRGNYAAAKQYGEQA
                     LGIYRHLFGPESGEVGMTEYILGSIAAEHGSFSEAKQLYMQSQRHIAAAYGPEDPRLG
                     QPMIDLAGLYLMTNRMIEAENLLRQVITLSDKPENRDPSRLSLALRHLGALLLTQKRL
                     DEAEPILKRSYETAQKELGDNHPQTAASLSTWAALHFAREHPEKAEPLYSQVIHIVER
                     SAGKSHRELAVALTNHAKALFAMKQFAHTEQQLRRAVAIYERQTGPSSIELANTLLSL
                     AVVIGKQRLSDAEPLFKRGLTITESAYGPDHPALAKALGLYAEFLRDQDRNAEADVLA
                     ERAESIRTRNMPERPTS"
     gene            9102..9575
                     /locus_tag="PK589_15065"
     CDS             9102..9575
                     /locus_tag="PK589_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20981.1"
                     /translation="MSKRLLSVVLSLGLLGMPGCFSHISYQEAPVPTADQTVLYISRT
                     DSPPRLRKASIFINDKLVADLASKEFTWITIAPGSYILHVKWSPDLAISAENINLHIR
                     EKEAIFVKIDSATVLFLGGSVVKVRLSGGNHPDQITQVWPLNYVEPKALTLTPLE"
     gene            complement(9694..10545)
                     /locus_tag="PK589_15070"
     CDS             complement(9694..10545)
                     /locus_tag="PK589_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20982.1"
                     /translation="MNDPREYSDLPPHVPLEVVGLVPAAGMAKRLQPFPCSKEVYPVG
                     FAMDQKSGLPRPKVAAQYLLEKFKTAGITKAFLVIKDGKWDIPNYFREGDLVGLSLAY
                     LVIAGSLGPPDTLDRAYPFLAQKRVAFGFPDILFGPDDAYVRLIQRQEETGADVVLGL
                     HLIEDPRVWDMVDCDEEGRIHALVMKPASTTLTYGWCCAVWTPVFTDFLHRFLRADET
                     KRNLDRLASKANDPSGDLAVGIVLQAAMKAGLVMQSVKFPGARYLDIGTPENLLKAVR
                     EFGGKIG"
     gene            complement(10542..11768)
                     /locus_tag="PK589_15075"
     CDS             complement(10542..11768)
                     /locus_tag="PK589_15075"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ20983.1"
                     /translation="MPADRNDDIDNALREHLAWWGLRHIESDAAYFAWQREVFTPEDL
                     AALHQSIEAKRAATDGASAEIAFYDLTAQSRFIPALYSQRYEYYLEVGSRIAAQLPGA
                     QTILDAGCGIGLLTTFYAQRCPDATVVGIDRSSASVTRARQRAQELGLSNVRFECLDL
                     DQHELSGRYDLIVATHTLLQAEQDPGLPSRDWQDFNRALDAQAQSEFEQRTGVGLRLD
                     RLRNVLAGNGRMILFEKTRQLARRVPFQRALAARGLALHLPPEPTRYRSIEEVTDDGP
                     LYVTGLVSPSSALSWNEAPEPDGAPPLHVQQLRSLRVDPEQPLYENHEASAQQAWLDL
                     PAKRVVHQMTRKEADGRQLHVEWGRAGEFAYLYCANTFDQRQLVVVDQARAAMVETYY
                     KEIVGEQSTSQQRMMK"
     gene            complement(11807..13174)
                     /locus_tag="PK589_15080"
     CDS             complement(11807..13174)
                     /locus_tag="PK589_15080"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNJ20984.1"
                     /translation="MMVEVPVKLYLDKVLKTAREAVRPTSLLSGPIRDKALRGMATAL
                     AEAEEPILAANETDVDAVGKSMTGYENRERVRDAVARIRMTADDVKALVDRLHHIADL
                     PDPLGEVLGRHEEPNGLQVSRVRVPIGVIGIVSELSPLETIDALALCLKSGNVCVFRG
                     SPDWTHTQHVIAAALTKAMHDAGIPKGACTIIDRQEKEAALELIKSGKALDAIIPRGG
                     AGLRKVVQEQAKMPILCHDGGITHVYIDDDVEIPLAQNIVVNSKVQNPSAPNSVDTLL
                     VHQGIARPLLSALILRLLDEFKIDIYGCPKTVSLMGQMLMTGHKAVKPAQETDWNKQF
                     QGPSMAIKMVPGFEEALAHIAQHGPSHTCVIVTKSYESAMRFSKEVDAGSVLVNASSR
                     LNAGDSLGFGADIGLSSTRHHARGPIGLNQLTCEKYVVFGSGQLRHPHPVPLAYEDAI
                     MLKRP"
     gene            complement(13356..14627)
                     /locus_tag="PK589_15085"
     CDS             complement(13356..14627)
                     /locus_tag="PK589_15085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20985.1"
                     /translation="MERWYRSVIRFVQAEPQELRPLAWSFGYFFCLLCGYYILRPVRD
                     EMAIQGGVHNLPWMMTGTFLTLLAVTPLFGWLSARYSRYRLLLAVYLFFIANLIGLYF
                     LMTSGLYIEWVARGFFVWLSVFNLFVVSVFWSFMADLFTPAQGARLFGMIAAGGSTGA
                     LFGPLITTGLTYLFPVPVLMLASAAFLAACIGCIYRLEMWSRSRSLFHRENSGAPLGG
                     GFLAGVRLVWSSPYLLGICGYLTFLTMTATFLYFEQVRLVAQQFDTPEARTRLFSMLD
                     FATNILTWLTQLLLTNHVVARFGLAAALLFLPAVSLLGFLGIALWPGLVVYVSFSVLR
                     RVGEYALSKPAREVLFTVVSREEKYKAKNFIDTAISRAGDASTGWLVSGLKALSVTTA
                     QIAWVLVPLMVLWGWLGRWLAAQQRKQIGPG"
     gene            14748..15779
                     /gene="ltaE"
                     /locus_tag="PK589_15090"
     CDS             14748..15779
                     /gene="ltaE"
                     /locus_tag="PK589_15090"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HNJ20986.1"
                     /translation="MIDLRSDTVTKPSPAMREAMARAEVGDDIYGEDPTVNRLQEVGA
                     ALVGKRAALFVPSGTMGNQLSIRAQAEPGREVIVEAQSHVIRYEQGAAAALAGVQLHW
                     VTGTHGLMTAEQVQRAIRPKDPSSIQSALICLENTHNAGGGRVYPLSTIQAIRAVATA
                     HGIPMHLDGARLFNAVVASGVPAAEFAKHFETVTFCLSKGLGAPAGSLIATDDLSMME
                     RLRRFRRMYGGAMRQSGILAAAGLYALDHHIARLSHDHANAKRLAARLQQIPAVTIDP
                     SLVETNILFFEVHSTKFSIPAFVAALKHEGVLINAVGGATCRAVTHLDVSAEAIEEAA
                     DQIAHLLNK"
     gene            15857..16084
                     /locus_tag="PK589_15095"
     CDS             15857..16084
                     /locus_tag="PK589_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ20987.1"
                     /translation="MSLHAVSFQQISRILDVTDALGLNREWVEIPLSPETPGIVRKLP
                     NGKLEIVVDADQPFDAWLGTLEQQIRRVQAA"
     gene            16103..16909
                     /locus_tag="PK589_15100"
     CDS             16103..16909
                     /locus_tag="PK589_15100"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNJ20988.1"
                     /translation="MESHLATSSPQSSPALPSSEELKAELLDIPEPPEMPPAVTPPGY
                     DDALAGAVKYVRAKRGGDLVGIVLVGSGARRAVTAHSDIDLIVLVKGPADGQEMIRVG
                     DRLVDIRYGEHTTVAEDLTYSPRLAPLLRKGRVLYDHEDIAAQLIAKAAQRFRQGPPP
                     ATIHERIRLKAECLHWLGKVEDLRDKPNTAQYLLQLFFEECLSAFFRTRGLWFTAPVD
                     TLRFLASREPALGEVAAQFLSAATLHDRLMAGRRFAETLFRDIPLPPRVD"
     gene            17065..>17449
                     /locus_tag="PK589_15105"
     CDS             17065..>17449
                     /locus_tag="PK589_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNJ20989.1"
                     /translation="MELGFVGLGKMGMNMVTRLQQGRHRVVAYDRAPDVVKQAEGKGC
                     VGAASLSDLVSKLTAPRAVWIMVPSGAPTEDTIQAISALLQPGDTIIDGGNTRFHDDT
                     RRAAELQKKGIHYVDVGTSGGIWGLQ"
     assembly_gap    17450..17549
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17550..18113
                     /locus_tag="PK589_15110"
     CDS             <17550..18113
                     /locus_tag="PK589_15110"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HNJ20990.1"
                     /translation="KGIHYVDVGTSGGIWGLQVGYCLMIGGETEPVQRLTPIFTTLAP
                     EQGWAHMGGHGAGHYVKMVHNGIEYSMMQGYAEGFELMAKSDYRLDLGKIADVWMHGS
                     VVRSWLLELAVGALKQDPKLEQLKGYVQDSGEGRWMIQDAIDKDVPVPTLTAALFTRF
                     RSRQDESFAEKMLAALRNAFGGHSVRR"
     gene            18139..>18749
                     /locus_tag="PK589_15115"
     CDS             18139..>18749
                     /locus_tag="PK589_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNJ20991.1"
                     /translation="MSSPTTPLACKPVPEAVPPVEPCTLVIFGGSGDLARRRLIPAVY
                     NLLLDGLLPAKYAVIGLGRKPMTDDEFRNLVKEGIVKHSRQALAEDTWDTFKTHLFYI
                     QGENEDAQTYATLRTKAEEVEQAMQLPGNRIFYLSIPPSSFASVCEGLAQAGLATSAS
                     AASPYSRIIVEKPVGRDLASAQQINEVTGKVFDESQIFRIDHYL"
BASE COUNT         3943 a         5777 c         5333 g         3596 t
ORIGIN      
        1 gaccgtcgaa ctggtgagca cgaatgtcga acggggtttc atcgacttct cgcgagtcgc
       61 ctcctgagcg gaaggcatca cagaagcagc actccgaagc ctcacgttca cgatctgatt
      121 caagggcccc agaccacctc tggcacattg gtgcctgcga tcaccgaaaa ggattccgca
      181 ccgagcgtgt aggctcccgc cgggagcagg ccggagaaga actgctctcc aggatctgtg
      241 tccgcgcccg ttcaatgctt gggtcggcgt tttttgaacg ccgcttccag caacgctctg
      301 gcatgaccga tcatcttcgt ctgttctgcc accatctgat tcaaggtcgc catctcggtt
      361 ctctcttaat cgcgcatacc catgaccagc tggtgaacgc tcggccgcaa ctgtcatcct
      421 ctcgaaccaa atgttcactc cgcgtctaat gccgcttata gcacagtgcg tcacactcga
      481 ccatgctcac aggacgtgag gtacgacgta ccgtgatagg agcgcctacg caacttcacg
      541 tggcccgcac ttcgtctacg cttctcttcg tcgccggagt gactcagcat gatacaacct
      601 gcctttcttc cgcaaaaaaa tgtccggaag ggttgacagc gcaacgtagg ataaggtgta
      661 ctcagatgcg gtcacgacgt tccacctcat tggggcccat gtgatcggca acaccagggc
      721 tctcagcgtg gcccaattcc cgaaactcct cgccgggaca ttggcgacct gacagtcact
      781 tcaacgcatc gcaggcctcc cggctcccac gcatctcgac agatgcgcgc ggctcggctt
      841 cgtccgtgac gtgcgggacc acctggccgg tcagagcaca gggcatattc accattcatt
      901 cagcgagaag gaccctcgcg agatgacccg cagtgccact ccggtaatgt cgctctcccc
      961 gaatctggac tttctgcgcg cgatcgcagt tctcctcgtg tacttcttcc acttgttcat
     1021 cacgactggt ccgcgcttgc cggacttcct cgcgcaattc ggcgtcattc tcttttttgt
     1081 tcataccagc ctggtgttga tgttctccct gcagcggatc gacctcaagg ggcagagact
     1141 gtttgcgacg ttctatctac gccgcctgtt tcggatctat ccgctcagca tcgtgtgcgt
     1201 cagtgtcatc atcttgcttc agcttccccg ggctccctgg tggccatgga cacaaccgga
     1261 cacctcgacc attctggcga atcttctgct gtataccgag ttcacctata aacctgtcgt
     1321 gacctcggtg ctctggagtc ttccttatga agtcgccatg tacgccgttc tcccattctg
     1381 ttacctggcc gggaaggcct atgggatcag aggaattctt gctctttggg gtctcgccgt
     1441 ggtgggcggc ttcgtccagc catacatctc ctggcgtctc ggcctggcgc aatttgcccc
     1501 ctgcttcatt gccggagtgg cttgctactt tatggggtat ggagctcgcc ggcgcccgct
     1561 gccattttgg ggctggccac tgatccttct tgccgccgcc ggcattcaag gatttgggag
     1621 ttactacgaa tatgagcagg tctcgcgatg ggtcctgtgc ctcatgatcg gtctcaccgc
     1681 gccgttcttt gccgaattga accggccgat gttgaacaaa gccacggaat tgatcgctcg
     1741 ctattcatat ggaatctatc tgacccatct ccatgcgcaa tgggcggcac tggtcctcct
     1801 caaagatcaa ccggccgccg ttcgatatag cgtcctggtc gtgctcagcg tcggcctgcc
     1861 cgtagcgctc tatcatatgg tggaagcacc aatgatccgg ctgggcgctc gcctggcagc
     1921 gaatttgcca acgaaatccg ttcccgtcgt cagaccggct tcgccgcaac gggcgctccc
     1981 tgcaccggca gaagctgcac cgtacggcag gccctcccag cccgtacggc ccaggccagg
     2041 gcaagcgccg gctgtccgca cgggtaaaag tgctccggct ccggcctccg cgaggtaata
     2101 ccctccgcgt cccttcgtct tcagttcctc cgtgatagaa ccacacccct gtgcctccct
     2161 tcaccttcgg ggtgcacgcg gcatcaattg acaggctcgc gcaccaggcg tacccttcgc
     2221 gcccatggcc cgcacgggtc cagacgttcc ccgatgcggc atgcgacaca ttcgccatcc
     2281 attcaccatc caacgagagg ttttcatgtt gaccagattc ctgttgtgtc ttgtcctggc
     2341 tgcagccacg ctacccaccg cgggacttgc actcgccgcc gaacgcacga acgttgaata
     2401 ctcggccgat caaatcatcg agtctgaaga tggagccacc aaaagccgta tctattcgac
     2461 accgaccaaa gaacgccgcg acatgtctca aggcggcgcc gcgatgacga tgatcacccg
     2521 ccatgacaaa catgtcagct ggacggtcat gccggaagaa aaaatgtaca tggaagcgcc
     2581 ggccactaag caggggcagg ataaatccga tctttccggc taccagattg agcagacggc
     2641 attgggagag gagaccgtca acggtgtcgt catgaataaa agcaagatca tcatgaccgg
     2701 caacgacggg acgaagatgg gcggtttcat gtggaccacg aaagaaggca ttctggccaa
     2761 gattgacgcc ctggccgtcg agaagggaca gaaagcgcgc ttcaagatgg agcagaccaa
     2821 tattcagatc ggcaaacaac cggccgaact ttttgaaatt cccaagggct tcgaaaagct
     2881 ggatatgggc ggcatgggca tggatatgct gaaagggatg atgggacgct aaggaggcag
     2941 tcgtgacacg aatcacatct tggcatctcg tgtgtgccgt cgcgcttggc ttgtcgggca
     3001 ctcccgtctg ggcagactcc ctgtgtgcgg agaaggcgct acaggcagcg gcagatgcgc
     3061 agttgaagaa ggccgaagag cttgaacggg ccggccaacc acgcgaggcc ttttccgccg
     3121 cgaccaaagc gaacggcgac tgtgtcagcg atactcaacg ccacgaaggg gtgcagcaac
     3181 gcaccgccaa ggccattggc gcagaagagg aaaagaaggc acgcctggag gacgcgttta
     3241 catggtacgt gcgcgcgaag gcacccgccg atgcgggccg catgcaacgc aggctcgtcg
     3301 aggccaaacc ggacgacatc aacacgctga gccacgccat cgactacttc tcccagcaga
     3361 acgacaaggc gcaggaagaa gcgatgcgca cccgggctcg caaaaacgtc gagcaggccc
     3421 tcgccgagga agaaaagcga tttgcctcat tcagcaggga ttcattgcat gaacttgggc
     3481 gggcgcgcga ttgggcctat tacgccaaaa ccggagagga ccgcattcgc gcgcgggcgg
     3541 cacaacgcgg cgataccctc gcggccgagg acggtcgaaa gttcctccgt ctcgccatgt
     3601 cctattacga gcaggccgac cgtccggacg gcgtcaaaaa aatacgcgag aaggcgcgaa
     3661 tgctggcgaa gcggcatgaa gccaaaggtg aaggcgagat cgcggcggag ttttacgcga
     3721 tggccggcga cgacaagcag gccgataccg tccagaagca aaccgagcag cgccaggcgc
     3781 aggccgaaga agggcgcaag aagaccttca aaaaagagca ggcagatctg gagaaggcac
     3841 tcggcttctg agcagccctc ctcgcgagaa actaatgctc gcgttcttct cgtgcaggcc
     3901 accgatggcc tgcgtgtgtg cttgctgcct caacgccccc cttgcctgat cgtcccggtt
     3961 atgggataag cgcggcgaac aatcctcgtc atgcagagat tgtaacggat ccttcgtcgg
     4021 cacatccgga tgaccggcgc tccacgggag acgaccgacg aggcaaccct tcgtcaacac
     4081 tacgatcaga cgtcatcagg cggcacgaga ccagcaggag cacattatgg cgaaaaaaca
     4141 atcgggcacg gtgagctggg cgacatcggc agagtgggac gttcgcacac tggagcccgc
     4201 tcgtggcgga cggcggggcg cgaccgaacc cccggacccc tttctggtga atctcacggc
     4261 aaccaaacgc tgggaagtcg agcagactct cacagcgacg cccaaagcca gacgcggcga
     4321 gaccgcccct gcgccactca ccctggatgt cgaaggcgac ccggacgacg tctatatcgt
     4381 catgacgcgt tatgcgtcag gagccatccg cttccacatg ccgaacgaac cggcccggcg
     4441 cggacgacgg aggagcgcac gaaccctcta ccgattttcg attcccgttc ctgccgcacc
     4501 tctcgccgag ccaggcggcc gccggggatt catcagtgcc gccatcaagg ccgtggtctt
     4561 gaaggtcgcc ggcaaactcg ccgattatgc gctcgccaaa cttgccttgt tgtgggaaac
     4621 cgccacctgg aaatataaag gacgccggga aggatggctg tccgtcacgg gcgaagcgct
     4681 tggtggccaa caaatgctcc cggccgctga tttgtcatcg ctcagcacca ctggccgcaa
     4741 tctgctcttc ctgcacggca ccttttccag cacgacggcc gccttcaatg gattggccac
     4801 aacccggggc agccgcggca aaacattctt cgacgagctg caggatctct acggcaaccg
     4861 catctatgga ttcgaccact tcaccctcag ccgcacaccg gaagaaaacg tgcgcatgtt
     4921 gctggaagcc ctgcccgatc gcaccaccac cttcgatgtg atcacccatt cgcgcggcgg
     4981 attggtcctg cgccacctcg tggaacgtcg tgaccggttc ggggcgctgg cggaccgctt
     5041 cgccgtcggc cgggccgtgc tggtcgcaag cccgaatggc ggcactccac tcgcatcacc
     5101 ggaccatgtg acgcaataca ccaactggct gtccaacgtg ctcgagctgt ttcctgacaa
     5161 tcccttcacg accggtgtgg aattcgtgag cgaggcgctg tcgtggatcg cccggcgact
     5221 ggtcggtagt ttgcctggtc tggcctctat ggataacaac ggcgagatca tccaggcatt
     5281 acaggcggca ccgaacccgc caggtcaagc ctattccgcc ctcgtcgcca atttcgaacc
     5341 ggatgcgggc ttacttcaac ggatcatcga tgcgggcgcg gatctgtttt tccccaccgc
     5401 caacgatctc gtcgtcccga ctgaaggcgg atggcgagtg gatccaggca gcggctccgt
     5461 ccctgccatt cccggggacc gcatcggttg tttcggagtc ggagggaatt tgtctcagca
     5521 gcagccggtg aaccatgtga actttttcca ggatcccggc acggtggatt ttctggtccg
     5581 cgcgttgcgt ggtgaatcac aattgcttac gccgattgac tgcaatggcg acctgccgac
     5641 cggacgacgg cgaggtgcag cccttcagaa aacaccgccc gccgctgtac cgacagtccg
     5701 ccgcgaagaa cagccaacaa cgaggccgac gcttcccgca gtgcaggtga atccgccagt
     5761 cgtcgcggtc gagccatacc ccgaaccaga cgacgtgttt catatcgccc tgatcgctcc
     5821 caaagcaggc gcggatgagg cgcagctcat cgccaccttc cggaatgccc gcgtcatgga
     5881 acacctgcac accggcgggt ccggcaagac aaccgccaca ggccgccggg gaaccagcga
     5941 tcatgcgccg acgcagtggg atcgtatcaa ggccggacag gcgcacattc acggctatat
     6001 caatggtgat cccgcttacc cgcaactccc ggatgagacc gcgctccagc aaatgggtag
     6061 cgccctcttc atggccctct ttcccggaca ggttcgccgg ttgtatgacg cagcgcgatc
     6121 cgaacaggtc agccagcggc tcaatctgat tttcacctcc gacatcggct ggattgccga
     6181 tcagtcgtgg gaattcattt atgacccaga ccggcgcacg ttcctcgcat tggaagaagt
     6241 caatttcacg cgcaatgtgc tgacggccat tcccgccgaa cgtattccgg ccagacccac
     6301 gatgcggatc ctcgtcgtgg tcgcgcagcc tttgggactc ggcgccctgt cggtggaaga
     6361 agaggccgat gtgatccgca gcggattccg ccgcctgctc gatgcgggac tggccgaggt
     6421 tgaactcctg ctcgacgcca cgccggaatt gttgcaccgg gcgctggagt ctgcggccgc
     6481 cccgatcgac gtgctgcact tcatcggaca cggggaatac aacgagcaga ccgactgcgg
     6541 atacttgatc ttcgagaatc aagacggcgg cgaacagcga ttggactcct ccacgctccg
     6601 tcagatcgtc tgccggcgcg ggatacgtct ggtctgcctg aacgcctgcg aaaccggccg
     6661 cggcgggcga caggatttca gccgcggtgt ggcgcaggcg ctgatcgccg gcggcgtccc
     6721 tgccgtggtc gccaatcaat atccggtgtt ggatgtgtcg gccactgcct tctcccgcca
     6781 cttctactgg gccctggcca tgggacatcc catcggcgat gcggcccgcg aggcgcgcgt
     6841 gtcggtcaac tatgcgatct ccggagaagc catcgactgg gcagtgccgg tcgtctttgc
     6901 gcgtaatccc gcacaacggc tctgcgtgcc gcgcaccgct gccgggtatg agcggagccg
     6961 cagggcggtg gtacgccagc ggcggcgcgc ggtagaagac cgcatcaaga tcggcatctg
     7021 gaacgcgcat cgcatgatcc cgcacctgcc cgacatctgc gagcgtctca ccaaagccca
     7081 ggatcggtac gccttcgaga ccgtatcctt ccctgctccc atcggcatct ggcgccggga
     7141 gcaggagaag gatcaggcat ttgtggtcgc cgaaacgttg tacgaccggt tgaagggcaa
     7201 accgaaagag ttgggcgtgg agacgctcat ctgcatgatc aactttccga tgaaggacgc
     7261 gcgaacgaga tacctgtatt actggaggcg agacccgctg atcgtcacgt cgacctttgg
     7321 attactggaa caattgaacg agcgggagtt caccgttgag cgcatgatgg ctcatctggc
     7381 ggcggcggtc gtcgcaggaa tcccgccgca tccccggcgg gtgggacctg ctgactgtcc
     7441 cctcttctac aatgaacagc gggacattcg tttgatcgcg ggccggctca cgttctgtgc
     7501 caactgtcga aaacaattca aagacaaacc ggcgcaggaa cgcctgcggg ctgccgagcg
     7561 acttcttgcc gcctatccgt agacggcaca ttggtcattc ggacttgtga cactcccgca
     7621 cggccttgag caaattctcc ggcgtgccga tgtcaaggta gcgcgcgccg gggactttca
     7681 cgctctatgt ataagcctgc atcgttggca aatgagcccc tgagcagcgc gattttgagt
     7741 gggaaatgaa aactccggga gtctgaattt ccgcgacggg agggaccatt agcggtagat
     7801 tattgctgtg tctaccaaga ttaagacatt cgtcctctgc tgcagtactc ttgtactgac
     7861 ggcctgccca caacctcacg ctgttccgcc tccctcgttt ccgccgtcat gggaaacgct
     7921 cattcgcaaa ggcgacatcg aacaggagta ttggcgatat cctgaagccg aatactacta
     7981 ttcccaagcc gtgacacgcg cccaggaatt cggagccgat gacgctcgcc tggcaaggtc
     8041 gctacgaagc ctgtcttccg ttgtctcaag ccgaggaaac tatgcagcag ctaagcagta
     8101 tggggagcag gcacttggca tatatcgaca tctcttcggg ccggagagcg gtgaagttgg
     8161 catgaccgaa tacatactgg gcagcattgc cgcagagcac ggctcattca gcgaagccaa
     8221 acaactctac atgcaatcgc agaggcacat cgctgcggct tacggccccg aagatcctcg
     8281 cctaggccag ccgatgatag acttggccgg gctatatctg atgaccaatc gcatgatcga
     8341 agcagaaaat ctgcttcgac aggtcatcac actgtcggac aaacctgaga accgcgatcc
     8401 atccagactg agtctggctc ttcgccatct cggcgccctc ctcctcacgc aaaagcgatt
     8461 ggacgaagca gagcccatct tgaaacggtc gtatgaaacc gcacagaagg aactcggcga
     8521 caatcatcct caaactgccg ccagtctttc tacctgggca gcccttcatt ttgctcgtga
     8581 gcatccggaa aaagcggagc cactctacag ccaggtcatc cacatcgtag aacgaagcgc
     8641 cggcaagtcc caccgagagc tggccgtcgc attgacgaat catgcaaaag cgctgttcgc
     8701 catgaagcag ttcgcgcaca cggagcaaca gctccgtcga gcagtcgcga tttatgagcg
     8761 acagactggt ccaagttcca tagagcttgc gaatacgctg ttgtctttgg cggttgtaat
     8821 tggaaagcag cggctttctg acgcggagcc tctcttcaag cgtggcttga cgattacaga
     8881 aagcgcctac ggccccgatc atcccgccct cgccaaagcc ttgggcctct atgcagagtt
     8941 tctccgggat caagacagga atgctgaggc tgatgtcctc gcggaacggg ccgagtcgat
     9001 tcgaaccagg aatatgcccg agcgccccac ctcctaatgc atacttcttg gtcagatgcg
     9061 agatacggtc acccctcgcg ggatgcagga ataaaggttc tatgtcaaaa aggttattga
     9121 gtgtcgtact gtcactcggt ctgctgggga tgccagggtg tttctcccat atttcttatc
     9181 aagaggctcc ggtaccgaca gcagaccaaa ccgtgctcta catttctcgg accgactctc
     9241 cgccgaggct acgtaaagcc tccatcttca tcaatgacaa gctggtggct gatctcgcct
     9301 caaaagaatt tacctggatc accattgcac ctggttcgta catcctgcac gtgaaatggt
     9361 caccggattt ggctatctcg gctgagaata tcaatctgca catccgcgag aaagaagcca
     9421 tctttgtaaa aatcgattcg gccacggtct tgttcttagg tgggagtgta gtgaaggttc
     9481 ggctatctgg cggcaaccat ccggatcaaa tcacgcaagt ctggccgttg aattatgtgg
     9541 aacccaaggc acttacattg acgccgctcg aataatccgc cgacgaaaag agtgggctgt
     9601 atacctacag aatctagctg ttgctcgtcc tcagctaccg atctgctcac tgggcacagt
     9661 ccgcaacgcc gcaactagcg agaacaatct gcatcaacct atttttccgc cgaactcccg
     9721 cacggccttg agcaaattct ccggtgtgcc gatgtcaagg tagcgcgcgc cgggaaattt
     9781 cacgctctgc atcacgagcc ctgccttcat tgcggcctgc agcacaatgc ccacggctaa
     9841 atccccgctc gggtcgttgg ccttgctcgc gagccggtca agattgcgct ttgtctcatc
     9901 ggcccgaaga aagcggtgga gaaaatccgt gaacaccggc gtccacaccg cgcagcacca
     9961 cccgtaggtc agagtcgtgg aggctggttt catgacgaga gcatggatgc gaccttcctc
    10021 gtcacaatcc accatatccc acacgcgcgg atcttcaatc agatgtaacc cgagcacgac
    10081 gtccgccccc gtttcctcct ggcgttggat caaccggaca taggcatcgt caggcccgaa
    10141 cagaatgtcc ggaaatccaa aggccactcg cttctgggcc aggaatggat aggcacgatc
    10201 gagcgtatca ggtggtccga gcgacccggc gatcacgaga taggccagcg acagaccaac
    10261 caggtcgccc tctcgaaaat aattggggat atcccatttg ccgtccttga tcacgaggaa
    10321 ggccttggtg attccggccg tcttgaattt ttccagcaga tactgtgcgg ccactttggg
    10381 acgcggcagc ccgctctttt gatccattgc gaacccgacc ggatagactt ccttgctaca
    10441 aggaaacggc tgcaggcgtt tggccattcc cgccgccggc acgaggccta ccacttctaa
    10501 cggcacatgg ggaggcaggt cggagtactc acgcggatcg ttcatttcat catcctctgt
    10561 tggctcgtag actgctcgcc gacgatttcc ttgtagtacg tttccaccat agcagcccga
    10621 gcctggtcga ccacgacaag ctgacgttga tcgaacgtgt tggcgcaata gagataggcg
    10681 aattcgcctg cccgtcccca ctccacatgc agctgtcgtc catcggcttc tttcctggtc
    10741 atctgatgca cgacccgttt cgcgggaaga tccagccagg cctgctgcgc ggacgcctcg
    10801 tggttttcgt ataacggttg ttctgggtct actcgaaggg accgcaactg ttgcacatgc
    10861 agtggcgggg caccgtccgg ctcgggcgct tcattccatg acagagcgct cgacggggaa
    10921 accaaacccg tcacgtacaa aggaccgtcg tcggtcactt cttcgatgga acgataccgg
    10981 gtcggttcag gcgggaggtg cagtgcgagg ccacgtgccg ccaacgctcg ctgaaaggga
    11041 acgcgccgag cgagttgtct ggttttctca aagaggatca ttcgaccgtt cccggcgagc
    11101 acgttgcgca gccgatccaa ccggagcccc accccggtcc gctgctcgaa ctccgactga
    11161 gcctgagcat ccagggctcg gttgaaatcc tgccagtcac ggctgggaag gcccggatcc
    11221 tgttcggcct ggagcagcgt atgcgtggcg acgatcagat catagcggcc gctgagctca
    11281 tgctgatcga gatcgagaca ttcgaagcgc acattgctca gtcccaattc ttgcgcccgc
    11341 tgtcgcgcgc gggtgacaga ggcagacgaa cgatcgatgc ccaccacggt cgcatcggga
    11401 cagcgctgtg catagaaggt ggtcagcagg ccgataccgc atccggcgtc caggatggtc
    11461 tgggcgcccg gaagttgcgc ggcaatccgt gagccgacct caagataata ttcgtagcgt
    11521 tgactgtaga gggcgggaat aaatcgggac tgcgccgtca aatcgtagaa ggcgatttca
    11581 gcggacgccc cgtcggtcgc cgctcgcttc gcttcgatgg attggtgcag ggcagccaga
    11641 tcttcggggg tgaacacctc ccgttgccag gcaaaatacg cggcatcgga ttcgatgtgc
    11701 cggagtcccc accaagcgag atgctcacgc agggcgttgt cgatgtcgtc attccggtca
    11761 gcaggcacaa tgccctcttg ccggaagagc catcacgatc ggtgcgttac ggccgcttga
    11821 gcataatggc atcttcatag gccagcggaa caggatgcgg atgccggagt tgcccgctgc
    11881 cgaacacgac atatttttca caggtcagtt ggttgagccc gatgggccct cgtgcgtgat
    11941 ggcgggtcga actcaagccg atatccgcgc cgaatcccag actgtctccg gcattcaatc
    12001 gggacgaggc attgaccagg accgaccccg catccacttc tttgctgaac cgcatggcgg
    12061 actcatagga ctttgtcacg atgacgcagg tgtggctggg accatgctgg gcaatatgcg
    12121 ccaacgcctc ctcgaagccc ggcaccatct tgatggccat gctgggtccc tgaaactgct
    12181 tgttccagtc cgtctcctgc gccggtttga ccgccttatg ccccgtcatg agcatttgcc
    12241 ccatcaagga cacggttttc gggcagccgt aaatatcgat tttgaattca tccagcaagc
    12301 ggaggatcaa cgccgacaag agcgggcggg cgattccctg atggaccagc agcgtatcca
    12361 ccgaattggg cgccgaggga ttctgcacct tggagttcac gacgatattc tgcgccaggg
    12421 gaatttccac atcatcgtcg atatagacgt gcgtaattcc gccgtcatgg caaaggatcg
    12481 gcatcttggc ctgctcctgc accaccttcc tcagccctgc accgccacgc gggatgatgg
    12541 catccaacgc cttaccggac ttgatcagtt ccaaggccgc ctctttctcc tgccggtcaa
    12601 tgatcgtaca ggcgcccttc gggataccgg cgtcatgcat ggccttcgtc agagcggccg
    12661 caatcacgtg ctgcgtatgg gtccagtccg gtgagccgcg gaagacacaa acgttgcccg
    12721 acttcaggca gagggccagc gcatcgattg tttccagcgg tgacagctcg gacacgatgc
    12781 cgatcacgcc gatggggacc cgcacccggc ttacctgaag accgttcggt tcttcgtggc
    12841 gccccagcac ctctccaagc ggatcaggga gatccgcaat gtgatgcaat cgatccacga
    12901 gcgccttgac gtcatcggct gtcatacgga tccgggccac ggcatcccgc acacgttcac
    12961 ggttttcata tcccgtcatc gactttccaa ccgcgtccac atccgtttcg ttcgccgcca
    13021 ggatgggctc ttccgcttct gccagagccg tcgccatacc gcgcaacgcc ttatctcgta
    13081 tgggaccgga aagaagcgac gtgggacgta ccgcttcacg cgccgtcttc aatactttat
    13141 cgaggtagag cttaactgga acttcaacca tcatgtcccc aattggtgag ctggtgagtg
    13201 ggcgtccggt gaatcgtcac agggtgccgg gaatcgtcag gacagcatca ttacaggcct
    13261 tcaacgatcc gcgccggact ataccatgcg atttttttgt gagtcaaagc agcgtggtcc
    13321 catctctgga ggcgacagag gccaggaagg gaggcctaac ccggcccaat ctgtttgcgt
    13381 tgttgcgccg caagccatcg gcctagccaa ccccagagga ccatgagcgg gaccaggacc
    13441 caggcgatct gtgccgtggt aacgctcaac gctttaaggc cggagaccag ccagccggtc
    13501 gaggcatccc ccgcgcgcga gatcgccgta tcgataaagt tcttcgcctt atacttttcc
    13561 tcgcgactca ccactgtgaa cagcacctcg cgcgccggtt tcgaaagcgc atattccccg
    13621 actcgacgca gcactgagaa gctgacgtac acgactaatc ccggccagag ggcgatgcct
    13681 aaaaacccga ggaggctcac cgccggcaga aacagtaagg ctgcagccag gccgaaccgg
    13741 gccaccacat ggttggtgag caagagctgc gtcagccagg tgaggatgtt ggtggcgaag
    13801 tcgagcatgg agaacaggcg cgtgcgggcc tccggagtat cgaactgttg cgccaccaga
    13861 cgcacctgtt cgaaatataa gaaggtagcc gtcatcgtca aaaaggtcag gtaaccgcag
    13921 ataccgagca gatacggcga cgaccacacg aggcgaacac cagccagaaa gcctccgccc
    13981 aatggggctc cgctgttctc ccggtgaaac agagaccggc tgcggctcca catttccaat
    14041 ctgtagatgc atccgatgca ggccgcgaga aacgcagccg aggccagcat caacaccggc
    14101 accgggaaga gatacgtgag gccggtcgtg atgaggggac cgaacaatgc gccggtgctg
    14161 ccgcctgccg caatcattcc gaagaggcgc gcgccttgcg ccggcgtaaa caggtcggcc
    14221 atgaaactcc agaacaccga cacgacgaac aggttgaata ccgagagcca gacaaaaaac
    14281 ccgcgcgcca cccattcgat gtacagaccg ctcgtcatca aaaaatacag gccgatgaga
    14341 ttggcaataa agaagagata gacggcgagg agcaatcgat agcgggaata ccgcgcagac
    14401 aaccaaccga acagcggcgt gacagcgagc aaggtgagaa acgtgcccgt catcatccag
    14461 ggaagattgt gcaccccgcc ctggatcgcc atttcatcgc gcacggggcg aagaatgtag
    14521 tagccgcaga ggagacagaa aaaatatccg aacgaccagg ccaacggccg cagttcctgc
    14581 ggctcagcct gcacaaaccg gatgactgac cgataccatc gctccatggg gctccgaatt
    14641 ggggccagtg tagcaggccc cggcacaccg gcaagaagaa tttttgctag aatgcacccg
    14701 cggttcgtcc tgaagaacca cactccactc ctgattgagg cccatccatg atcgatcttc
    14761 ggagcgatac ggtcaccaaa cccagccccg ctatgcgtga agccatggcc cgcgccgagg
    14821 tgggtgatga catttatggc gaagacccca ccgtcaatcg tctccaggaa gtgggtgccg
    14881 cactcgtcgg caaacgggcc gcactcttcg tgccctcggg cacgatgggc aaccaactgt
    14941 ccattcgagc gcaggctgaa ccaggacgtg aagtgattgt tgaagctcag tcacatgtca
    15001 ttcgttatga gcagggcgcg gcggcggcgt tggccggcgt acaacttcat tgggtgaccg
    15061 gcacgcacgg gctcatgacg gccgagcagg tgcagcgcgc catccgaccg aaagatccct
    15121 ctagcatcca gagcgcactc atttgtcttg agaatacgca caacgccgga ggcggccggg
    15181 tctacccgct ttcgactatt caggccattc gtgctgtcgc cactgcccat ggaataccga
    15241 tgcatctcga cggggcccgc ctgttcaatg cggtcgtggc ttcgggcgtg ccggccgcgg
    15301 aattcgcgaa gcacttcgaa acggtgacct tctgcctctc caaaggactc ggggcaccgg
    15361 ccgggtcgct gattgcgacg gacgatctca gcatgatgga gcgccttcgg cgatttcgtc
    15421 gcatgtatgg aggcgccatg cgccagtctg gaattctcgc cgcggcgggt ctctacgcct
    15481 tagaccatca catcgcgcga ctcagtcacg atcacgccaa cgccaaacgg cttgcggccc
    15541 ggctgcaaca gattccagcc gtaaccatcg atccatccct cgtcgaaacg aacattctgt
    15601 tcttcgaggt gcatagcacg aaattttcca tcccggcgtt tgtggcggcc ctcaagcatg
    15661 agggcgtgct gatcaatgcc gtcggcggcg caacttgtcg cgcggtgacc catctcgacg
    15721 tgtcggcgga ggcgattgag gaagccgccg atcagatcgc gcatctgctc aacaagtaga
    15781 ccgagcaggc cttgcgcctc acgttgacag tctctgtccc cccacctaca atgccaacaa
    15841 gagtagagag gattgagtgt cgcttcacgc cgtgtcgttt caacagatca gtcgcatcct
    15901 cgacgtgacg gatgcgctag ggctgaatcg cgagtgggtg gaaattcctc tctcgccgga
    15961 aactcccggc atcgttcgca aacttcctaa tggaaagctt gagattgttg tcgatgccga
    16021 ccagccgttc gacgcgtggc tcggcaccct cgagcaacag atacggcgtg tgcaggccgc
    16081 ctgaccgcgt tgaaggatag acatggaatc acacctggca acctcttccc cgcagtcttc
    16141 ccccgcgctc ccgtcctcgg aggagctgaa ggctgagtta ctggatattc ccgaaccacc
    16201 ggagatgcct cccgcggtga cgccgcccgg ctacgacgat gccctcgccg gcgcggtgaa
    16261 atacgtccgc gcgaaacgcg gcggcgacct ggtgggtatt gttctcgtcg gatccggcgc
    16321 ccgacgcgcg gtgacggcgc acagcgatat cgatctcatc gtcctggtga agggtccggc
    16381 cgacgggcag gaaatgatcc gggtgggaga ccggctggtc gatatccgct atggtgaaca
    16441 taccaccgtc gccgaagatc tcacctactc tcctcgtctc gcacccctgc ttcgcaaagg
    16501 acgcgtgctc tacgaccatg aggacattgc ggcgcagttg atcgccaagg cagcgcaacg
    16561 atttcgccag ggcccgccgc cagcgacgat acacgaacgc attcgcctca aagccgaatg
    16621 cctgcactgg ctcggaaagg tcgaagacct gcgcgacaaa cccaacaccg cgcaatatct
    16681 gctgcagctc tttttcgagg agtgcctctc tgcatttttt cgaactcgag ggctctggtt
    16741 cacggcaccg gtagacaccc tccggttcct ggcgtcccgc gagccggcac tcggcgaggt
    16801 ggctgcccaa tttctcagcg cggcaaccct tcacgatcga ttgatggccg gccgacgatt
    16861 tgccgagacc ctgtttcgcg atattcccct tccaccccgc gtggactaac agggctccat
    16921 caccccgtgc caaggcgaca gcgcttgcgt gggtgtccga ccgtgatagc atcagacctg
    16981 caccgaacca ttggagcggc actccgtcct tggccggctc cgtaccagtc atcacatacc
    17041 ggtcttcacc agagagggtg acctatggaa cttggattcg tcggactcgg caaaatgggc
    17101 atgaacatgg tgacccgcct gcagcagggc cgtcaccgcg tcgtggcata cgatcgtgca
    17161 cccgatgtcg tgaagcaggc cgaaggcaag ggctgcgtcg gcgcggcctc cctgagcgat
    17221 ctcgtctcca aactcaccgc tccccgcgca gtgtggatta tggttccctc gggagccccg
    17281 acggaagaca ccattcaggc catcagcgcg ctgctgcagc cgggcgatac gattatcgac
    17341 ggcggcaaca cccgcttcca tgacgatacg cgccgcgccg ccgaactaca gaaaaagggc
    17401 attcattatg tcgatgtggg taccagcggc gggatctggg gattgcaagn nnnnnnnnnn
    17461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna agggcattca ttatgtcgat gtgggtacca
    17581 gcggcgggat ctggggattg caagtcggct attgcctcat gatcggcggg gaaaccgaac
    17641 cggttcagcg gctgacgccc atcttcacta cgctggcgcc ggaacagggc tgggcccaca
    17701 tgggcgggca cggtgcaggg cactacgtca agatggtgca caacggcatc gagtacagca
    17761 tgatgcaagg ctacgccgag ggattcgaac tcatggcgaa aagtgactat cgcctcgacc
    17821 tgggaaaaat tgccgatgtc tggatgcatg gcagcgtggt tcgctcctgg ctcctggaat
    17881 tagccgttgg cgcactgaaa caggacccca agctggaaca gttgaaaggc tatgtgcagg
    17941 attccggcga aggccggtgg atgatacaag acgccatcga caaggatgtg cccgtcccga
    18001 ccctaacggc cgcgctgttc acccgcttcc ggtcgcggca ggacgaatcg tttgccgaaa
    18061 aaatgctggc cgcgttgcgc aatgccttcg gcggacacag cgtccggcgc tgaccttcac
    18121 caaaggacta tcgagactat gtcctcccct acaactcccc ttgcgtgcaa gcccgttccg
    18181 gaagccgtcc ctcccgtcga accctgcacg ctcgtcattt tcggcgggtc cggtgacctc
    18241 gcccgccgcc gccttatccc ggccgtctac aacctattac tcgacgggct gttaccggcc
    18301 aagtatgcgg tgatcgggct gggccgcaaa cccatgaccg atgatgagtt ccgcaatctg
    18361 gtgaaggaag gaatcgtcaa acattcgcga caggctctgg cggaagacac gtgggatacg
    18421 tttaaaacgc atctctttta cattcaggga gagaacgagg atgcgcaaac ctatgccacc
    18481 ctccgcacca aggcggagga agtggagcag gcgatgcagt tgcccggcaa tcgcatcttt
    18541 tatctgagca ttccccccag ctccttcgcg tcggtctgcg agggattggc ccaggcaggt
    18601 ctggcgacct ccgcgtccgc cgcctcgccc tactcgcgaa tcatcgtcga aaaaccggtg
    18661 gggcgcgacc tggcctctgc ccaacagatc aatgaagtga ccggcaaggt gttcgatgaa
    18721 tcgcagattt tccggatcga tcactatct
//
LOCUS       DAOQDS010000072        18591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1184_length_18591_cov_20.731172, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000072 DAOQDS010000000
VERSION     DAOQDS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18591
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1184_length_18591_cov_20.731172"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..3231
                     /locus_tag="PK589_15120"
     CDS             262..3231
                     /locus_tag="PK589_15120"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNJ20992.1"
                     /translation="MIKAWLLDEMFRFDRRYLTAEGSQSEWGAGDSVAEEEDLPPAPT
                     GVAAKAGNGRVMITWDAVPDAMYYNLYFMTTKGVQIKFSELTRPIASAEDFKTVIGVT
                     KEKGTCIEGAQSPFMHDDLANGTCYHYVVTVVTQKGESPESQEVMAIPAPYLIAKVIG
                     QEGVDEGEFSSPTGIAVDKDGNLYVADTDNHSIQKFDKEGKFLGRWGGEAGSAEGNYY
                     YPRGLTTNNDGQVYVADTGNNRVQRLDTDGNPMKSWGKFGFAWRGADMNKFDAPWGVT
                     TDQEGNIYVTDTNNARIQKFKGDGTPLLKWGRDGSFDGAFFFPRGVAVDFVGNTYVAD
                     EGNNRIQKFDTRGSFLTKWGREGSGPGQFKAPWGVTCDALGNVYVVDQGNHRIQKFDG
                     NGTFLCAWGNRGKTEGQLNFPSGVAVDKEGAVYVVDSGNHRVIKYVPTDEELNRGKEE
                     RERAQVVSEYPAPGNLAIKPGDTETFLSWMEVPKATSYNLYFHTSPNLTVEGGTKIEG
                     VTSPYNHTGLTNDQAYFYALTAVYEDGTESTLSEEVSATPVLIDITAPQTPYAVINHG
                     AFMTNSPEIVVTISATDLDTGVAAYYISENPMTPMAGTPGWVDVPPAIKFGATIPFIL
                     SPGDGQKTVIVWFKDLGNNISTPASATILVNTSGYLCVSKWGKPGRGASLLHGGEFMA
                     PMYGLAIDQQGSIFVVDNGNNRIQKFDRNGNFIILWGNFGAANANFHNPTGIACDAKG
                     DVYVVDTNNHRVQKFDGKLGGYMMKFGSRGNGEGQFNAPWGIAIDRVRGYIYVVDSAN
                     FRVQKFDMTGEFIMSWGSFGNGDGQFYFPRGIAVDQADGTVYVVDMGNHRVQKFDTST
                     NVLPQLLTKWGGSSEAGHASSPLAQEAGQLRSPWGIAVDGQGDVYVTDTGNHRLEKFD
                     REGNFISQWGGFGGGDGQFNFPYGIVVDSRGSVFAVDSGNTRVQQFMPAEEGSERLQE
                     EADAAAELGQIDKTTRV"
     gene            3270..4736
                     /gene="guaB"
                     /locus_tag="PK589_15125"
     CDS             3270..4736
                     /gene="guaB"
                     /locus_tag="PK589_15125"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNJ20993.1"
                     /translation="MLEKEIRLGLTYDDVVLVPAKSQVLPTEVDTRTRLSRNIHLNIP
                     IVSAAMDTVTEARLAIAMAQEGGIGIVHRVLSPADQAAEIDRVKKSESGMILDPITIS
                     PDQTIRDAHDLMARYRISGIPVTKGGKLVGILTNRDLRFETRMDLKVSQVMKRDKLVT
                     APEGTSLEKAREILHEHRIEKLPVVNKQFELKGLITIKDIEKRIKYPNACKDAHGRLR
                     VGAALGVGPDTKDRVDLLVKAGVDVVVVDTAHGHSQAVLDTVKMVRKAHPKLDIIAGN
                     IATAQAAKDLVKAGVDAVKVGVGPGSICTTRMVSGAGMPQLTAIADCARALAGSGIPV
                     IADGGIKYSGDITKALAAGASVVMLGSLLAGTEEAPGETVLFQARTYKVYRGMGSIGA
                     MERGGGDRYGQGGRPTPKLVPEGIEGRVPYKGLLAPHIYQMVGGVKSGMGYCGCKTIP
                     DLQQKATFIRQTLAGLREGHVHDVIITKEAPNYRTDWE"
     gene            4824..6380
                     /gene="guaA"
                     /locus_tag="PK589_15130"
     CDS             4824..6380
                     /gene="guaA"
                     /locus_tag="PK589_15130"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNJ20994.1"
                     /translation="MELWHDRILVLDFGSQYTQLIARRIREAQVYSQILPCTVSLATV
                     LAYRPKGIVLSGGPSSVYDKKAPKIPKQLFDQGIPILGICYGMQLVTHLQGGEVEKAP
                     HREFGRAELQLDDRSDLFKGIGRGGSTVVWMSHGDRIERMPPGFRSIAHTDNSPIAAM
                     KRHDAKQRIYCLQFHPEVAHTAEGATILKNFVYDICGCKPTWTMRSYVDTAVEQIRQQ
                     VGTERVICALSGGVDSSVAAALTHRAVGDQLTCIFVDNGVLRAGEREQVEKTFASQLH
                     LNMRILDRTTQFMTALKNVTDPERKRKIIGRLFIKNFEAESKKLKGIKYLVQGTLYPD
                     VIESVSFKGPSATIKTHHNVGGLPTKMKLKLVEPLRELFKDEVRVLGQELGLPDEIIW
                     RQPFPGPGLAIRVLGAVSKERLAILRGAETIVDQEIRAAGLYREIWQAFAVLLPIRTV
                     GVMGDQRTYEYVIAIRAVTSLDGMTADWAKIPNDVLGRMSSRIINEVKGVNRVVYDIS
                     SKPPSTIEWE"
     gene            6455..7711
                     /locus_tag="PK589_15135"
     CDS             6455..7711
                     /locus_tag="PK589_15135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNJ20995.1"
                     /translation="MTVRLQKLIAGTGIASRRKAEELIVSGRVTINGHVVKELGTKVD
                     PERDHVKVDGKHLRAAQPYVYLILNKPKNVMSTLDDPEGRPTVKDFLRGVTVRVFPVG
                     RLDFDSEGLMLLTNHGDLAQALLHPRYHVPKTYLIKVKGVLDDTKIETLERGVKLEDG
                     FTAPAKINKVSRAESNSWLEVTIHEGRKHQVKRMIEAVGHSVIKLTRVRMGPLLLGEL
                     ASGEYRFLTDREANRLRELVEDRVARAEHPELDASGKPVRWMPPTEPAPPRPPKPERS
                     GRGPKSQRPDFGPRSVRGSGGANSGRPGRGALKRPLSPGAKFRRPAKTTGFQRPRPGA
                     VPQGDDRVPMANPPDSVRKPRPSGAGFQRGSFDRKSQGAGRKTPGNHSGSDQKFQQNR
                     RSGPSGSRPGQRGPARQSRNSTRRRA"
     gene            7708..9471
                     /gene="aspS"
                     /locus_tag="PK589_15140"
     CDS             7708..9471
                     /gene="aspS"
                     /locus_tag="PK589_15140"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNJ20996.1"
                     /translation="MKIRTHRCGELTKAHVGQQVVLNGWVQRRRDHGMVLFIDLRDRT
                     GLTQVVFNAERNAAVHQAAHTLRSECVVSVTGQVMARPEESRNPHLPTGEIEIFVDAV
                     EILNEAKTPPFMIEDDIEITESLRLKYRYLDLRRPRMQRLLGLRHGIMQAVRAFLNAE
                     HFLEVETPVLTKSTPEGARDYLVPSRVNPGMFYALPQSPQLFKQVLMVSGVDRYYQIA
                     RCFRDEDLRNDRQPEFTQIDLEMSFVDREQVMSLMERMIVSVFKDAGGVQLPVPFPRM
                     TYAEAMGRYGSDKPDLRFDMPLYDVTAFAAKSDFKVFKDAATKGGLVKALIVSGGATI
                     ARSRIDALGETAKSFGAKGLAWLKVTAEGQLESVIAKFLDAQAFAAALPEAKPGDLVL
                     FGADKPAIVHDVLGRIRLLLGEELNLIDKTAWKPLWVTEFPLLDYSPEEKRYIFMHNP
                     FAAPMDEDVPFLDTEPLKARAKAYDMVLNGSEIGGGSIRNHRSDIQFRILDLLGIGKD
                     QAQAKFGFLLEALEYGAPPHGGIAFGLDRLIMLLGGADSIRDVIAFPKTQRAQCPLTE
                     APSAVSTDQLLELRIKLDLVE"
     gene            9489..10652
                     /gene="aroC"
                     /locus_tag="PK589_15145"
     CDS             9489..10652
                     /gene="aroC"
                     /locus_tag="PK589_15145"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNJ20997.1"
                     /translation="MAGNTLGRVFTVTSFGESHGPAIGCVVDGCPPGLALSTEDIQQD
                     LDRRKPGTSRHVTQRQESDHVEILSGVFEGRTTGTPIALLIRNEDQRSRDYGNLIDTF
                     RPGHADYTYWQKYGIRDHRGGGRSSARETAVRVAAAAIARKWLYEKHGVMIRGYLSQL
                     GPIEAPFQSWDTVSRNPFFSANAEVVERLDSFMDELRKAGDSVGAKITTVAEHVPVGW
                     GAPVYAKLDADLAGAMMSINAVKAVEIGAGFASVAQRGSEHGDELTPEGFVTNHAGGI
                     LGGISTGQDVVVTIAIKPTSSIRVPRRSIDKTGKPQSVETNGRHDPCVGIRATPIAEA
                     MMALVLMDHALLHRAQNADVKTATPKIAGSINRLGSSASSKASSKPNAGPAEV"
     gene            10779..>11963
                     /gene="rnhA"
                     /locus_tag="PK589_15150"
     CDS             10779..>11963
                     /gene="rnhA"
                     /locus_tag="PK589_15150"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNJ20998.1"
                     /translation="MIEIYTDGACSGNPGPGGWGVLLRLDGEETELCGGEPATTNNRM
                     ELLAVIEALQWFTHPVKARVYTDSQYVQKGISEWIHSWKRRGWKTASKEPVKNEDLWR
                     RLDALASGHTIEWHWVRGHNGHVDNERVDALARAGLEQSRRAGKPVTRPVATPVSAPV
                     KAAPVTVTSRPIVDIRHATVYRGETRVFSDFSFALREGEHAAIVGPNGAGKSTLLKLL
                     SGDVHPLALDDTTVQLFGQERWSVWDVRKQLGIVSHDLQRDYLICAEGLNVILSGFYA
                     SNDTYDHQTFSQEQVARAREVMRELQIDQLAGRTFGHLSTGEQRRFLLGRALVHDPSV
                     LVLDEPTSGLDLKACFQYLDLLRAQIRKGKTVLLVTHHLHEIPPEIERVVLLKEGEVV
                     ADG"
     assembly_gap    11964..12063
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12064..12743)
                     /locus_tag="PK589_15155"
     CDS             complement(<12064..12743)
                     /locus_tag="PK589_15155"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNJ20999.1"
                     /translation="MSRSPLRYSHQLMRCAVLTVASVAFVAGLFTVSASAFDAAALDR
                     AKLATIGVLEDTQDQRTPDKPGKILVRGTGFHLRDGYIVTARHAAEKQDVTTGTVIQK
                     QIHVLTTDLHEIPADLVGDSAFMDVVVYRVAEAHRAKLLANVAFAPGDVQPGQDVFTV
                     GYPMGWGPTMSFGHLGNTNTFLQTVDTRLIQADVAACSGNSGGGLFNDKGEVVGIMHA
                     IIQTERDDS"
     gene            12873..13427
                     /locus_tag="PK589_15160"
     CDS             12873..13427
                     /locus_tag="PK589_15160"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNJ21000.1"
                     /translation="MKKILQALIIGVAVVAAGCATPVIPESLEPQVDKAVTFSQIVES
                     PDSYRGKVVVVGGEILKAKALKGGTQLEVLQLPLDSDQEPVTDRMESKGRFLALQKEF
                     LDPATIAEGTRVTVVGEVTGSSVEKMDEADYRFPTLDVKHLHRWDAKRAEERSAPGPW
                     WNVFGGVGFGGGGTRSGGGISIGF"
     gene            complement(13493..14359)
                     /locus_tag="PK589_15165"
     CDS             complement(13493..14359)
                     /locus_tag="PK589_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNJ21001.1"
                     /translation="MRWQGQRESQNIEDRRGMGAARSGAGIGLGGLVLVLAVSFLTGT
                     NPLTLLNLLTGVQEITGPAEPDRPGPTGAPSDNLGKFAAVVLADTEDTWRALLPQMGS
                     AYQDPQLVLFTGAVRSACGTASSAVGPFYCPGDRKVYLDLSFFQELARRLGAPGDFAQ
                     AYVIAHEIGHHVQNLLGLADQVTRQQQRSSPAERNALSVRMELQADCFAGVWGYHASR
                     NRQLIEPGDFEDGLHAAAAIGDDRLQQMGQGYVQPESWTHGSSEQRVAWLRRGLQSGD
                     PHVCDTFAGGRS"
     gene            14591..15211
                     /locus_tag="PK589_15170"
     CDS             14591..15211
                     /locus_tag="PK589_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21002.1"
                     /translation="MWSHLSITHARRGTLFQLCSVALIALLYLDGCRPAPVATLPPTL
                     KPSLAWPECPKIGGMYELQGEALPGSMNFYRNRDSKLTLNAMLGVAAPADLAQESVRV
                     ELVHDDTLELVNRYDGAITAHQLDLQPEDRVVCEPNRIRIHRTRAEGGVAEEPLRNIS
                     EYAQELTVEGDGSLLVKTRILNQSKSAFWSTPIPPEEYAARFRRLD"
     gene            15333..15926
                     /locus_tag="PK589_15175"
     CDS             15333..15926
                     /locus_tag="PK589_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNJ21003.1"
                     /translation="MPVTARRPSRTLALHKPYDVLPCFTDPDGRPTLSRYIDVPDVYP
                     AGRLDRDSEGLLLLTSDGTLAHHITDPRHLLAKVYLVQVERIPDAAALKRLEAGVLLG
                     GKRTRPAQARLLEEAPRLPDRPVPIRFRKHIPTAWLELTLREGMNRQVRRMTAAVGHP
                     TLRLVRVAIGSITLGDLQPGQWRELTKQEVADMFHSA"
     gene            16205..16747
                     /locus_tag="PK589_15180"
     CDS             16205..16747
                     /locus_tag="PK589_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21004.1"
                     /translation="MWGRAIVTNRWSLLLLLGIGFSHVTPVFGANPDSVPVADMRHIK
                     KFVAVEVQTQGTAEKLGISAAELTDVTRVTLLNKVPGIVLEASSGPSLDATERLNQLG
                     FFTCEVWTVGEQYIAAYHVDCNAGSYTPQKTPGSLWNQAILGYGPRDEVSDAVRKGVR
                     AMVELFASTFAGVRADGGGR"
     gene            complement(17452..18360)
                     /locus_tag="PK589_15185"
     CDS             complement(17452..18360)
                     /locus_tag="PK589_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNJ21005.1"
                     /translation="MPPRETNYFTENKKFYVVNYSDSPFNVSRDLRQDEWRLSVKGEV
                     KNPLSLGWRELLNRENFEQISTLMCIDTLPGGDSLGNARWRGISLKKLLQEAGVDEDT
                     TRDIVFRGADAYDDSIPLTRAMQDDVMLAFLMNGEKLPKEHGFPLRLLVPGLYGIKNV
                     KWIVEIEAYAGDYKGYWQRKGWTDDGTIKTFSRIDSPGHYQTLRGPVQRFRGIAFGGP
                     NSISRVEISLDAARTWDSCEIETPMSPYSWVIWNYTWHPPKRGKYQVAVRAVDSKGQP
                     QIAELIRPQPAGSSGLHTIIADVESV"
BASE COUNT         3936 a         4977 c         5599 g         3979 t
ORIGIN      
        1 tttgctgccg ggagtgcaga gcctatgata ttcgaaagaa atgcgtccat ccagctttgg
       61 ggggatggag acccaccagc ctctcgacct gatggaacgg gcttccggtg atcgatcaca
      121 ctgcgacgga attgtattga ggaaggctgt gaagcgcccc ggtcctgcct tgactccctt
      181 cttttcgctt tgctagactg cccgatctaa gttattgaaa catccgccaa tcttcaccaa
      241 tctcgtacca cgaggaacac catgatcaag gcttggctgc tcgacgaaat gtttcggttt
      301 gaccgtcggt atctcaccgc ggaaggaagt cagagcgagt ggggcgccgg ggactccgtt
      361 gcggaggagg aagatctgcc tcccgccccg actggagtag ccgccaaagc cgggaatggc
      421 cgggtgatga ttacctggga tgccgtgccg gacgccatgt attacaactt gtatttcatg
      481 acgacgaaag gcgtgcagat caagttttca gaactcaccc gccctatcgc cagcgcagag
      541 gattttaaaa ccgtcatcgg cgtcacgaag gaaaaaggga cctgtatcga aggggctcag
      601 tcgcccttca tgcatgacga tctggcgaac ggaacctgtt atcactatgt cgtgacggtg
      661 gtgacgcaga aaggggaaag ccccgagtct caagaagtca tggcgattcc agctccgtac
      721 ctgattgcga aagtgatcgg ccaggagggc gtggacgaag gagagttcag ctctccgacg
      781 ggcatcgcag tggacaagga cggcaatctc tacgtggccg atacggacaa tcattccatt
      841 cagaagttcg ataaagaggg gaagtttctc ggccgttggg gcggagaggc gggtagcgcg
      901 gaaggcaatt attattatcc ccgcggcctc acgaccaata acgacggcca ggtctacgta
      961 gccgataccg gtaacaatcg tgtgcagcgg ctcgataccg acggcaatcc catgaagtcc
     1021 tggggcaagt tcggatttgc ctggcgcggg gccgatatga ataagttcga cgcgccctgg
     1081 ggtgtgacta ccgaccagga aggcaatatt tacgtcaccg ataccaacaa tgcccgcatt
     1141 caaaaattca aaggcgacgg cacgccgttg ctcaagtggg gacgggatgg cagtttcgac
     1201 ggcgcgttct tctttccccg tggagtcgcg gtggacttcg ttggaaatac gtatgtggcc
     1261 gatgaaggca acaaccgtat ccaaaagttt gatacgcggg gcagcttttt gaccaagtgg
     1321 ggacgcgagg gtagcggtcc cggtcagttc aaggcgccct ggggcgtcac ctgcgatgcc
     1381 ttgggcaacg tgtacgtggt ggatcagggc aatcaccgca ttcagaagtt tgacggaaac
     1441 ggcaccttcc tctgtgcctg gggcaatcgc ggaaaaacgg aaggtcagct gaactttccg
     1501 tcaggtgtcg ccgtcgataa ggaaggcgca gtctatgtcg tggatagcgg caatcaccgc
     1561 gtcatcaagt atgtgccgac cgatgaagaa ttgaatcgcg gaaaagaaga gcgtgaacgg
     1621 gctcaggtcg tcagcgaata ccctgctccc ggcaatctgg cgatcaagcc tggtgatacg
     1681 gaaacgttcc tgagttggat ggaggtgccg aaagccactt cctacaatct ctatttccat
     1741 acctccccca acctgaccgt agaaggtggt acgaagatcg agggcgtgac gagtccctac
     1801 aatcataccg ggctgaccaa cgaccaagcc tacttttatg cgttgacggc agtctatgag
     1861 gacggcacag agagtacgtt gtcggaggaa gtttcggcga caccggtgct gatcgacatc
     1921 acggccccgc aaacgccgta tgccgtgatc aatcatggcg cgttcatgac gaactcaccg
     1981 gaaattgtcg tgacgatttc cgcgaccgac cttgatacgg gggtggccgc ctactatatc
     2041 tctgaaaacc cgatgactcc gatggcggga acgcccgggt gggtggacgt tccgccggcc
     2101 atcaagttcg gcgcgacgat tcccttcatt ctttccccgg gcgatgggca gaagaccgtc
     2161 atcgtctggt ttaaggatct cgggaacaac atttcgaccc cggcaagcgc caccattctc
     2221 gtcaatacat ccggctatct ctgcgtctcc aagtggggca agccgggccg cggcgcatca
     2281 ctattgcacg gcggcgagtt catggcgccg atgtacggtc tggccatcga tcagcaggga
     2341 tccatttttg tcgtcgacaa cggaaacaac cgtattcaga agtttgaccg taacggcaat
     2401 ttcatcattc tctggggaaa cttcggtgcg gccaacgcca actttcacaa tccgaccggt
     2461 attgcctgcg atgccaaggg cgatgtctac gtggtcgata cgaacaatca ccgcgtccag
     2521 aaattcgacg gcaaattggg cggctacatg atgaagttcg ggtcgcgtgg aaacggcgaa
     2581 ggccagttca atgcgccgtg gggcatcgcg atcgatcggg tgcgcggcta tatctacgtc
     2641 gtcgatagtg cgaacttcag agtccagaaa ttcgacatga ccggcgaatt cattatgtcg
     2701 tggggaagtt tcggcaacgg cgacggacag ttctatttcc cgcgcggtat cgccgtcgat
     2761 caggcagacg gcaccgtcta tgttgtcgat atgggcaacc atcgcgtaca gaagttcgac
     2821 accagcacga acgtcctgcc tcagttgttg acgaaatggg gaggaagttc tgaggccggc
     2881 catgccagca gtccgttggc gcaagaggcg gggcagcttc gttccccctg gggtatcgcc
     2941 gtggatggac aaggtgacgt gtatgtcacg gataccggga accatcgtct tgaaaagttc
     3001 gatcgtgagg ggaactttat ttctcagtgg ggtggcttcg gcggcggcga cggacaattc
     3061 aacttcccct acggaattgt ggtggattca cggggaagcg tattcgccgt cgatagcggc
     3121 aacaccagag tgcagcaatt tatgccggca gaagaaggca gcgagcggtt gcaggaagaa
     3181 gcggatgccg cagccgaact ggggcagatc gataaaacca cgagagtcta agggcagcgg
     3241 gcaggaagcc cgggagaggg tggcaacgga tgttagagaa ggagattcgg ctcgggttga
     3301 cctatgacga cgtcgtcctg gttccggcta aatcgcaagt tcttccgacc gaagtcgata
     3361 cccgcacgcg cctctctcgg aatatccatc tgaatattcc catcgtgagt gctgccatgg
     3421 acaccgtgac ggaagcgcgg ttagccatcg caatggccca agagggcggc atcggcattg
     3481 tgcaccgtgt gctctcgcct gccgaccaag cggctgaaat tgaccgggtc aaaaagtctg
     3541 aaagcgggat gattcttgat cccatcacca tctcgcctga tcaaaccatc cgtgatgctc
     3601 acgacctgat ggcccgatac cggatttccg gcatccctgt gacgaagggc ggtaaattgg
     3661 tcggcatcct gacgaatcgc gatctgcgat ttgagacgag aatggatctg aaggtctcgc
     3721 aagtcatgaa gcgtgacaag ctcgtcactg cgccggaagg caccagcctg gaaaaagccc
     3781 gcgagatcct gcacgagcac cggattgaaa agttgccggt ggtgaacaag cagttcgaac
     3841 tcaaaggctt gatcaccatt aaggacattg aaaagcggat caagtatccc aacgcctgca
     3901 aggatgccca cggccgcttg cgtgttggtg cggcgcttgg cgtggggcct gatacgaagg
     3961 atcgagtgga cctgctggta aaggccggcg tagacgtcgt cgtcgtggat accgcccatg
     4021 ggcattccca ggcggtgctt gatacggtca aaatggtcag aaaagcccat ccgaaactgg
     4081 atatcattgc cggcaacatt gcaaccgcac aggcagcgaa ggacttggtg aaggcaggtg
     4141 tggatgcagt taaagtcggc gtcgggcccg gttcgatttg caccacacga atggtgtcgg
     4201 gggccggcat gccccaattg acggccatcg ccgattgcgc cagggcttta gccggatcgg
     4261 gaattccggt gatcgcggac ggcgggatta agtattccgg cgatatcacg aaagccttgg
     4321 cagcgggcgc ctcggtcgtt atgctcggaa gtttgttggc tggtacggaa gaagctccag
     4381 gcgaaacggt cttgttccaa gcgcgcacct acaaggttta ccgtggaatg gggtccatcg
     4441 gggccatgga gcgtggcggt ggggatcgtt acgggcaggg tggtcgtccg acaccaaaac
     4501 tggttccgga aggtatcgaa ggccgcgtac cgtacaaagg tcttctcgcg ccgcacatct
     4561 atcagatggt tggtggtgtg aaatccggta tgggatattg cggatgtaag accattcccg
     4621 atttacaaca gaaggcgacc ttcattcgcc agacattggc cgggctccgc gaaggccacg
     4681 tacatgacgt gattattacc aaagaagcgc ccaactatcg cactgattgg gaataacacg
     4741 cgtagaacgg gcgagaggcg ttgccttccc cgttttcgtc agcccccgca ctgaacctct
     4801 aatctcgtac accgcgcaca ctcatggaac tttggcacga tagaattctt gttctcgatt
     4861 tcggctccca gtacacgcaa ctcattgcac gacgtatccg tgaagcgcag gtctattcgc
     4921 agatcctgcc ctgcacggtt tcgctcgcga cagtgttggc ctatcggccc aaaggtatcg
     4981 tcctgtccgg cggtccctcg agtgtctacg acaagaaggc tccgaaaatc cccaaacaac
     5041 tctttgatca aggcatcccg atacttggaa tctgctatgg gatgcagttg gtgacgcatc
     5101 tccagggcgg tgaagtggag aaagcgccgc accgagaatt tggccgggcc gagctgcaac
     5161 ttgacgatcg ctcggatctc ttcaagggta ttggacgcgg tggatccacg gtggtgtgga
     5221 tgtcgcacgg ggaccgcatc gagcggatgc cgccaggttt ccgttccatt gcccatacgg
     5281 acaattctcc cattgcggcg atgaagcggc atgatgccaa acagcgtatt tattgtttgc
     5341 agtttcaccc cgaggtagcc cataccgccg aaggtgccac gattctcaag aattttgtct
     5401 acgacatctg cggctgcaaa ccgacctgga cgatgcgctc ttatgtcgac actgctgtgg
     5461 agcagattcg tcagcaggtc gggacggagc gggtgatttg tgccttgagc ggtggggtgg
     5521 attcgtcggt ggcagcggct ctgacgcatc gtgcggtcgg cgatcaattg acctgtattt
     5581 tcgtcgataa cggggtgttg cgtgccggcg agcgtgagca ggtcgagaag acctttgcgt
     5641 cccaactgca cctcaacatg cgcatcctcg atcgtacgac ccagttcatg acggcgttga
     5701 aaaatgtgac tgatccggag cgcaagcgga agatcatcgg ccgcctcttc atcaagaatt
     5761 tcgaagccga atccaagaag ctcaagggca tcaaatatct tgtgcagggc acgctgtatc
     5821 ccgatgtcat cgaaagcgtc agcttcaaag ggccttccgc gaccatcaag acccaccata
     5881 acgtcggcgg gttgccgacc aaaatgaaac tgaagctggt tgagccgctg cgcgaattgt
     5941 tcaaggacga agtgcgggtg ttggggcagg agcttggctt gccggatgag atcatttggc
     6001 ggcagccgtt tcccggtccg gggttggcga tccgggtgct cggcgcggta tccaaagaac
     6061 gattggccat cttgcgcggg gccgagacga tcgtcgatca ggaaattcgc gccgccggac
     6121 tctatcggga aatctggcag gcctttgccg tgttattgcc gatccgaact gtcggcgtga
     6181 tgggcgatca gcgaacctac gagtatgtga ttgcgattcg ggccgtcaca agcctcgatg
     6241 gcatgaccgc tgactgggcc aagattccga acgacgtact gggtaggatg tcgagtcgga
     6301 tcatcaatga agtgaagggc gtcaaccgcg tcgtctatga catcagctct aaacctccga
     6361 gcacgatcga gtgggaataa cagtgctaga tatgccacct aaacggtgag tcacggcgat
     6421 ccctttacct gatacgttgt ccctttcaca gcatatgacc gttcgtctgc aaaaactcat
     6481 tgccggtacc ggtatcgctt cccggcgaaa agctgaggaa ttgatcgtgt ccggacgcgt
     6541 gaccatcaac ggccacgtgg tgaaagagct tggcactaaa gtcgatcccg agcgcgatca
     6601 tgtcaaagtg gatggcaaac atctgcgagc cgctcagccc tatgtctatt taatcctcaa
     6661 taagcccaag aacgtcatgt ccaccctcga tgatccggaa ggtcgtccga ccgtgaagga
     6721 ttttttgcgt ggcgtgacgg tgcgcgtgtt cccggtcggc cgattggatt tcgatagcga
     6781 aggactcatg ttgctcacca atcatggtga tttagcccag gcgctgctcc atccccgcta
     6841 ccatgtgccg aagacctatc tcatcaaggt caagggtgtc ctcgacgata cgaaaatcga
     6901 gacgcttgaa cggggagtca aactcgaaga tggtttcacc gccccggcga agatcaataa
     6961 agtcagcagg gccgaaagca attcgtggct ggaagtcact atccatgagg gccgcaaaca
     7021 ccaggtcaag cggatgatcg aggcggtcgg ccattcggtc atcaagctga cacgtgttcg
     7081 gatggggcct ctcttgctgg gcgaactggc atcgggggaa tatcggtttt taaccgaccg
     7141 tgaggccaat cggttacggg aactggtgga agatcgtgtg gcaagggccg aacatccgga
     7201 gttggacgcg tcgggcaaac cggtgcgctg gatgccccct acagagcccg cccctccacg
     7261 cccacctaag ccggaacgtt ccgggcgtgg ccccaaatct caacggccgg acttcggacc
     7321 tcgatcagtt cgaggttcgg gtggcgcgaa ctccgggcgt cctggtcgtg gtgcgctgaa
     7381 acgcccgctc agtcctgggg cgaagttccg ccgtcctgca aagacaacag gctttcaacg
     7441 gcctcggccg ggtgcggtcc ctcaaggcga cgatcgtgtc ccaatggcga atcctcccga
     7501 ttcggtgagg aagcctcggc cttcgggagc aggttttcag cggggctcat tcgacagaaa
     7561 atctcaaggc gctgggcgaa agacgccagg gaatcattct ggctcggacc agaaatttca
     7621 acagaatcgg cgaagcgggc cttctggctc gcgcccaggc cagcggggcc ctgcaaggca
     7681 gtcgcggaat tcaacgcgga gaagagcatg aagatcagga cccatcggtg cggggagttg
     7741 accaaggcac acgtcggtca acaggtcgta ttgaacggct gggtgcaacg gcgtcgtgac
     7801 cacggcatgg tgctgttcat cgatttgcgt gatcggacag gattgacgca ggtcgtgttc
     7861 aacgctgagc ggaacgccgc tgttcatcag gcggcacata cactgcggag tgaatgcgtt
     7921 gtgtcggtca ccggtcaggt catggctcgc cccgaggagt ctcgcaaccc gcatttgccg
     7981 acgggcgaga ttgagatctt cgtcgatgcc gtggagattc tgaacgaagc gaagacgccg
     8041 cctttcatga tcgaagacga catcgagatc accgagtctc tgcggttgaa atatcgctat
     8101 ctcgacctgc ggcgcccacg aatgcagcgg ttgctggggt tgcgtcacgg aatcatgcag
     8161 gccgtgcgtg catttttaaa tgccgagcat tttctcgaag tggaaacacc ggtcctgacg
     8221 aagagcactc cggaaggcgc gcgggattat ctggtgccga gtcgcgtcaa tccgggcatg
     8281 ttctatgcgc tgccgcagtc gccgcagttg tttaagcaag tcttaatggt cagtggtgtc
     8341 gaccggtatt accagattgc tcgttgcttc cgtgacgaag atctgcgcaa cgatcgccag
     8401 ccggaattta cccagatcga tcttgaaatg tcgttcgtag accgcgagca ggtcatgtcg
     8461 ctcatggagc ggatgatcgt gtctgtgttc aaggatgccg gtggcgtgca gttgcccgtc
     8521 ccgttcccac gaatgaccta tgccgaagcc atggggcgtt atggctccga caaaccggat
     8581 ctgcgcttcg atatgccgtt gtacgatgtc acggcctttg cggcgaagag tgacttcaaa
     8641 gtgtttaaag acgcggcgac caaaggcggg ctcgtgaagg ctttgattgt gtctggcggg
     8701 gcgaccatcg cgcggagccg tatcgatgcg cttggtgaga cggccaaaag tttcggcgcc
     8761 aaaggcttag cttggctgaa ggtcaccgcg gaagggcaat tggaatccgt gattgccaaa
     8821 tttctcgatg cccaggcctt tgccgcggcc ttgccggagg caaagcccgg ggacctggtt
     8881 cttttcggag cggataagcc ggccatcgtg catgacgtgc tggggcggat ccggttgttg
     8941 ctgggtgaag agctgaattt gatcgataaa actgcctgga agcccctctg ggtgaccgag
     9001 ttcccattgc tggactattc gccggaagag aagcgctata tcttcatgca caatcctttt
     9061 gcggcgccga tggacgaaga tgttccgttc ctggacacgg agccattgaa ggccagagcg
     9121 aaggcgtacg atatggtgct caacgggagt gaaatcggcg gcggcagcat acgtaatcat
     9181 cgcagtgata ttcagttccg gattctcgac ctgttgggta tcgggaagga tcaggcgcag
     9241 gccaagttcg gcttcttgct ggaagccctg gagtacggcg cgccgccgca cggcgggatc
     9301 gccttcggct tggatcgatt gatcatgctc ctgggcgggg ccgattctat tcgtgatgtc
     9361 attgccttcc cgaagaccca acgcgcgcag tgcccgctca ccgaggctcc atctgctgtc
     9421 agtaccgatc agttgctgga acttcgcatt aagctggatc ttgtcgaata gcaggagcca
     9481 tcccattcat ggccggcaac acgttgggcc gagtcttcac cgtcacctca ttcggcgaaa
     9541 gccatggacc ggccatcggc tgcgtcgtcg atggctgtcc gccagggctg gcactctcca
     9601 ccgaagatat tcagcaggat ctggatcgcc gcaagcccgg cacatcccgc catgtcacgc
     9661 agcggcagga atccgaccac gttgaaatcc tatccggggt tttcgaagga cggacgacgg
     9721 gtacgccgat tgccctcctc atccggaacg aagatcaacg cagccgggat tatgggaacc
     9781 tcatcgacac cttccgtccc ggccatgccg actacaccta ttggcagaaa tatggcatcc
     9841 gcgatcatcg gggcggcggg cgttcctcag cccgtgaaac cgctgtgcgg gtggcggctg
     9901 ctgcgatcgc gagaaagtgg ctatacgaaa aacatggcgt catgatccgt ggctatctca
     9961 gtcaactggg cccgatcgag gctccgtttc aaagctggga cacggtgagc aggaatccgt
    10021 tcttctctgc caacgccgag gtggtcgaac ggctcgactc ctttatggac gaattgcgga
    10081 aagcgggtga ctctgtcggt gcgaaaatca cgacggtcgc ggagcatgtg ccggtagggt
    10141 ggggtgctcc ggtgtatgcc aaactggatg cggacctggc cggcgccatg atgagcatca
    10201 atgccgtgaa ggcagtggaa atcggggccg gttttgcttc agtcgcccag cgaggttccg
    10261 agcatgggga cgaactcacg cctgagggat ttgtcaccaa tcatgcaggc gggattctcg
    10321 gtggaatttc aaccgggcag gatgtggtgg tgaccatcgc catcaagccg acctcgagca
    10381 ttcgcgtgcc gcgtcgttcg atcgataaaa ccggcaagcc acagtccgtc gaaaccaatg
    10441 gccgtcacga tccctgtgtc ggtattcgtg ccactcctat tgccgaagcc atgatggccc
    10501 tcgtgctcat ggaccatgcc ctcctgcatc gcgcccaaaa cgccgatgtg aaaactgcga
    10561 ctccgaagat cgccggctcc atcaacaggc ttggttcttc agccagctca aaagcatcct
    10621 cgaagcccaa cgctggccct gccgaagtat agcggcctgt catgtctgcg tcgtctgctg
    10681 acttctgctc gtgatgacac gggggagata tccgtggatg cgcactgagg agagtgattg
    10741 gtgaagtgtt tggtgagcgc tcagaggaac gtcctggcat gatcgagatc tacacagacg
    10801 gtgcctgtag cggaaacccc ggccctggtg gctggggagt tttgctgcgc ctggacgggg
    10861 aagaaacgga actctgtgga ggcgagccgg ccacgaccaa caaccggatg gagttgctcg
    10921 cggtcataga ggcgctgcag tggtttacgc acccggtgaa ggctcgagtc tacacggatt
    10981 ctcagtatgt gcaaaagggc atcagcgaat ggatccatag ctggaagcgc cggggctgga
    11041 aaacggccag caaagagccg gtcaaaaatg aagatctctg gcgtcgtctg gatgcattgg
    11101 cttccggcca caccatcgaa tggcattggg tcaggggaca caatggccat gtggacaacg
    11161 aacgagtgga cgcgctggcc cgcgccgggc tcgaacagtc gcgtcgagcc ggaaagccgg
    11221 ttacgcgtcc ggttgcgact ccggtttccg cacccgtcaa ggcggcacca gtcacagtaa
    11281 cttcccgccc cattgtggat attcggcacg cgaccgttta tcgaggagag acccgcgtct
    11341 tttcggattt ttccttcgcg ctgcgtgaag gagaacatgc ggcgatcgtc ggtcctaatg
    11401 gcgccggaaa gtctactctg ctgaagttat tatctggtga tgttcatccc ctggcactcg
    11461 atgacaccac agtgcagctg ttcggtcagg agcgctggag tgtgtgggac gttcggaagc
    11521 agttgggaat cgtgtcgcac gacctgcagc gggactattt gatttgtgct gaaggcctga
    11581 acgtcattct gtccggcttt tatgcgagca acgacacgta tgaccaccaa acgttcagtc
    11641 aggagcaggt cgctcgtgcc cgggaggtga tgcgggaatt gcagattgac cagttagctg
    11701 gccgcacctt cggtcatctg tcgaccggcg agcaacggcg gtttctcctc gggcgcgcgc
    11761 tcgtgcatga tccatctgtg ctggtgctgg acgagcccac gagcgggctc gacctcaaag
    11821 cctgttttca atatctcgat ctgctgcgcg cgcaaatccg gaaagggaag acggttctcc
    11881 tggtgacgca ccatctccac gaaattccac ccgagatcga acgcgtggtg cttctcaagg
    11941 agggtgaagt ggttgcggat gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnngagtcgt cgcgttccgt ttgtatgatg gcatgcatga tgccgacgac ttcacccttg
    12121 tcgttgaaga ggccgccacc ggagttgccg ctgcaggcgg cgacatcggc ttgaatgagc
    12181 cgtgtatcaa ccgtctgaag gaacgtgttc gtattgccca ggtggccgaa cgacatggtg
    12241 gggccccagc ccatgggata cccgacggta aagacatcct gccctggctg cacgtcaccg
    12301 ggagcaaagg ccacgttggc caagagtttg gcgcgatgtg cttcagcaac gcggtagacg
    12361 acgacgtcca tgaaggcgct gtcaccaacc aggtctgcgg gaatctcatg gaggtcggtc
    12421 gtcaacacat ggatctgttt ttgaatgacg gtgccggttg tgacatcctg cttttccgcc
    12481 gcatggcggg cggtcacgat gtagccatca cggaggtgaa acccagtgcc acgcactaaa
    12541 attttccccg gtttgtcagg cgtacgctga tcttgcgtgt cttccagaac gccgatggtc
    12601 gccagcttgg cgcgatctag tgctgctgca tcaaatgccg aggccgagac ggtgaagagg
    12661 ccggcgacga acgcaacgga ggcaaccgtc agtaccgcac aacgcatgag ctgatgtgag
    12721 taacgaagcg gggagcgtga catacgaact attcctttcg tgtggatgtc gtcaggtctg
    12781 tgcagtataa accagggccc gtgccggagg aaatgaatgt gttgtgcgcc tgcgccgtag
    12841 tatcaggcgc cgggctgggc tatactgagg tgatgaaaaa gatccttcag gcactgatca
    12901 ttggagtggc ggtcgttgcc gctggctgcg cgacgccggt cattccggaa tcactggaac
    12961 cgcaggtcga taaagccgtg acgttcagcc agatcgttga atcccccgat tcctatcgcg
    13021 gcaaagtcgt cgtcgttggt ggagagattc tgaaggcgaa ggccctgaag ggaggcacgc
    13081 agctggaagt cctgcagctg ccattggata gtgaccagga gcccgtgacc gaccggatgg
    13141 aatccaaagg gcgatttctc gccctacaaa aggagtttct cgatccagcc accattgccg
    13201 aaggcaccag agtgacggtt gtcggagagg taacggggag ctcagtggaa aagatggatg
    13261 aagccgacta tcgtttccct acgctggacg tcaaacatct ccatcggtgg gatgcgaaac
    13321 gagcagagga acgttccgcg cccggtccct ggtggaacgt gttcggtggt gtgggattcg
    13381 gcgggggcgg cacccgcagt ggcggcggca tcagtattgg attttaactc gccggcagtc
    13441 ccctagtctc ttcttccgct ctgtactccg ttatccagcc ttctgcactg gtctaagatc
    13501 gcccgccggc aaaggtatcg cacacatggg gatcgcctga ctgcagaccg cgccgcagcc
    13561 aggccacccg ttgctcggat gagccgtgag tccagctttc cggttgcacg taaccctgcc
    13621 ccatttgttg aaggcgatcg tcaccgatgg ctgcagcggc gtgcagtccg tcctcaaaat
    13681 cgccgggctc gatgagttgc cgattacgac tggcatggta tccccacacg cccgcaaaac
    13741 aatcggcttg taattccatg cggacggaaa gcgcgttgcg ttcggccggg gaactgcgtt
    13801 gttgctgccg ggtaacctgg tcagcgaggc ctagcagatt ctgtacgtga tgcccgattt
    13861 catgggcgat gacataggcc tgggcaaagt cgccgggggc gccaagccgt cgggccagtt
    13921 cctgaaaaaa tgagaggtcc agatagacct tacggtcgcc gggacagtaa aagggcccca
    13981 cggcggatga ggcggtgccg caggcggacc gtacagcacc tgtgaacagc accaactgcg
    14041 gatcttgata ggcactgccc atttgcggga gcagcgcgcg ccaagtgtct tccgtatcgg
    14101 ccaggacgac ggcggcaaac ttcccaagat tgtcgcttgg cgcaccggtt ggtccggggc
    14161 gatctggctc cgcgggaccg gtgatctcct gcacgccggt gaggagattg aggagcgtga
    14221 ggggattggt gcccgtcaga aagctgacgg cgagcacgag cacaagcccg cccaatccta
    14281 tacccgcgcc ggaccgggcc gcgcccatgc ctcggcggtc ttcaatgttc tggctctcgc
    14341 gctggccttg ccatcgcatg attggactcc ttggttgcgg cactgaccga cggtcgtctt
    14401 ttagctgtgg gaagagtcag ctgatggaaa catatttcgc atggctgggc aaggctcttg
    14461 ccaaccagat agccgaggag ctgcgggggg atgtctcaga ttggcgcagc ccgtgaacat
    14521 ttcggaacag ggggcctttt ccccttcttc tctctagaca ggccggtgag cgcttcgcta
    14581 gacttgaaga atgtggtcgc atctttccat cactcatgcc cgacgaggaa cgctctttca
    14641 gctctgttcg gtggcgctga ttgcattgtt gtatctcgac ggatgccgtc cagccccggt
    14701 ggcgactctg ccaccgacgt tgaagccgag tttggcgtgg ccggaatgtc cgaagatcgg
    14761 cgggatgtat gaactgcaag gagaagcgtt gcccgggtcg atgaattttt atcggaaccg
    14821 cgatagtaag ctcacgctga atgcgatgtt gggagtggct gcgcctgcgg accttgcgca
    14881 ggagtctgtt cgcgtggagt tggtgcatga cgacacgctg gagctggtca atcggtatga
    14941 tggagccatt accgcgcatc agttagattt gcagccggaa gatcgagtgg tctgtgagcc
    15001 taatcggatc cggatccatc gaacccgtgc ggagggtggc gtggccgagg aaccgctgcg
    15061 gaatatttcc gaatatgccc aggaattgac cgtggaagga gacgggagct tattagtgaa
    15121 aacccgtatc ctcaatcaga gcaagtctgc attttggagc acccccatcc ctcctgagga
    15181 gtatgcggcg cgcttcaggc ggctcgactg atcgcgagag tctggacctt ccttccgtat
    15241 cggtgacctc tcgtgcacat gggtatttca tcctgcgtca cctgttctgt cccctacaga
    15301 gccattacga ctgaagtcct ggagggcagc gcatgcctgt gacggctcgc cgtccttcac
    15361 gtacactcgc ccttcacaag ccctacgacg tcctgccttg tttcaccgat cctgatgggc
    15421 gccccacact gagccgttac attgatgtgc cggatgtcta tccagccggc cgattggatc
    15481 gtgatagcga aggactcctc ctcctgactt ccgacggaac gctggcgcat cacatcaccg
    15541 atccccgaca tcttctcgca aaagtgtacc ttgtccaggt tgagcgtatt cccgatgcgg
    15601 cagcgctaaa gcgtcttgaa gccggcgtgc tgctcggagg gaagcgcaca cggccggcac
    15661 aggcccgcct tctcgaggaa gctccgcgtt tgcctgaccg gcccgttcca attcgatttc
    15721 gaaaacatat tcccacagct tggttggaac tcactttacg cgaggggatg aaccggcaag
    15781 tccggcggat gactgcggcg gttggccacc cgaccttgcg actggtgcgg gttgcaattg
    15841 gttcgatcac cttgggtgat ctgcaacctg ggcaatggcg ggaactgacc aagcaggaag
    15901 tggccgatat gtttcattcg gcttagaatg cggtcgcttt gtgaacgggg cacgtgagat
    15961 tcgtgatcca cctcgatttg atttggcaca gtgctgagcc gaacgtgtcg cagagccagg
    16021 acaagtgaga gtgactctcc tacgttgttt tcagaatatt ctgacaagac tgcgtgaaga
    16081 tacgcatgcc gcatcctctc ttgaatgcat ctagatctgg atatggagcg tcaagctgag
    16141 gtatatcaag cgaggcgaat agggtcgaac tattgaatgg ttcttcagac gagggagatt
    16201 gattatgtgg ggacgcgcca tcgtgactaa caggtggtcc ttgcttctgc tgctgggcat
    16261 cggcttctca cacgtgacgc cggtcttcgg cgccaatccg gactcggtcc cggtcgccga
    16321 catgcggcac attaaaaaat ttgtcgctgt tgaggtgcag actcaaggaa cagccgaaaa
    16381 gctcggtatc agtgcggctg aattgaccga cgtaacgcgc gtcacgttgc tgaacaaggt
    16441 gccgggcata gtcctcgaag catcgagcgg tccatctctt gacgcgactg agcgcctcaa
    16501 tcagctcggg tttttcacct gcgaagtctg gacggtgggt gaacaataca tcgccgccta
    16561 ccacgtggat tgcaatgcag gttcctatac gccgcaaaag acccccggga gcctgtggaa
    16621 tcaggcgatc ctcggctacg gaccgaggga cgaagtctcg gatgcggtga gaaaaggtgt
    16681 gcgagcgatg gttgaattgt tcgcgtctac gtttgccggt gtgcgagctg atggaggggg
    16741 ccgctaaacg actctacaag tcatcagtgt tgttatctgc catacacccc acaatcaatc
    16801 ctcgatatcc ttcgattttc aattcgggtc agaaactctt cctagcagct ctgacgggta
    16861 ttgatcgctc tctcttggtc tgacggcgat ccttccgcca catgtgccgg taacgattca
    16921 tctcggttgc aaagccggac acggaggttc gtgtctgttc atttccgact gggcgcatcc
    16981 tgttgcggcc ctcggccggg cagcgagaat tttaatgcgg gagagtctga ctcgtctccc
    17041 cgcaacaact gacgcagtga acactcctgg ggcgggtggt gtgtgtgaat atggtctctt
    17101 ctgtctgcac atgtgaggcg tattcttttg cagcatggca tgttgaagtg agcttggatc
    17161 agggctttct cccagtcttc tccgggccga tggagtctgg tcgtgatgat ggcgctcgcg
    17221 cgtgttctac gcagtaaatc ctacggtgtg ccgcgaagaa gaaatatgag gcgacctctt
    17281 tcctgcgcac gatctgccta actcgctctt gatgtccggt cagtcgtgat ctgggaaaga
    17341 atttgtttta ggctcgcgct gggtttggcc cggaggtttg gtaggaaagc aagaattggt
    17401 aagtggtgat acgtatttat tgatagtcga atgttaggag gggttctgat tctagacgct
    17461 ttcgacatcc gcgataatcg tatggagtcc gcttgatccc gctggttgag ggcggatcaa
    17521 ttcggcaatc tgtggctgcc ctttgctatc tacggctcga acggcgacct ggtacttgcc
    17581 tcgtttgggc gggtgccagg tgtaattcca gatcacccac gaatagggcg acatcggcgt
    17641 ttcaatttca caggaatccc aggttcgcgc tgcgtccaga ctgatttcta ctctgctgat
    17701 cgaatttggt ccgccgaaag caatgccgcg aaatcgttgc actggaccgc gtagcgtctg
    17761 gtaatgacct ggcgaatcga tgcgagaaaa tgtcttgatg gttccatcat cggtccatcc
    17821 cttgcgctgc cagtacccct tgtaatcgcc ggcgtaggct tcgatttcga caatccactt
    17881 cacatttttg attccataca gccctggcac tagcaggcga agcggaaatc cgtgctcttt
    17941 cggcagtttt tcgccgttca ttaggaacgc cagcatgaca tcatcttgca tggcacgggt
    18001 cagtggaatg ctatcgtcat aggcgtcggc tcctcgaaac acgatgtctc gcgtcgtgtc
    18061 ttcatcgacg ccggcttcct gcaatagttt tttcaatgaa atgccgcgcc accgtgcatt
    18121 tccgaggctg tctcctccgg gcagggtatc aatgcacatg agcgtggaaa tttgctcgaa
    18181 gttctcgcgg tttaataact cacgccaccc tagggataac ggatttttta cttcgccttt
    18241 caccgacagg cgccattcat cctgtcggag gtcgcgagac acgttaaacg gggagtcgga
    18301 gtagttcacg acatagaatt tcttattttc cgtgaagtag ttggtctctc gagggggcat
    18361 ggcgaacatg cgtccgaaaa aagatccaac tccgtcacag ccacccgtga ggccggccgc
    18421 agccgcaatt ccagtgatct ttaggaggct tcgccgcttt atggtcaacc aattttccat
    18481 aagaaatact ctgcgatatt aaaaagatct tttcaaggct tgactccaga aatgcggatt
    18541 ggtataagag tgcacgtttg atccagccta gtggccgatg cggttgtaag a
//
LOCUS       DAOQDS010000073        17967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1260_length_17967_cov_19.730795, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000073 DAOQDS010000000
VERSION     DAOQDS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17967
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1260_length_17967_cov_19.730795"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PK589_15190"
     CDS             complement(<1..709)
                     /locus_tag="PK589_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21006.1"
                     /translation="MQDQAAVCTDTPTNQQAGQPPTPQNAVRSWLLRAVALFIKPAHF
                     FATFDDLARPVVLLVATLCLGVASMVDRIEQHILRAEMGQGVSGWSELSPWLLHSWGT
                     LWIALLVCGALNVPLFWYLGGWWYRLRLKWSGATALDSLRPRLLFVYSSLVYALPVVL
                     VMIGETLLFPNYRLARDAEGSWTLIFVLLSFWSVVVSYCGATRTFALARRKALLWFLL
                     LPWTLYAVELGLWMWLFE"
     gene            complement(987..>2197)
                     /locus_tag="PK589_15195"
     CDS             complement(987..>2197)
                     /locus_tag="PK589_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21007.1"
                     /translation="MNHRANGYLVSIEPDEPKMEFILPLKPTSFRGNAGYVFKNYDRL
                     KQAGVTEFQLTLGLVFADLPLQIFDINQIGVDGNYAPWLAHVDRIIDQVMDRRLSVLW
                     DIYNEPDLATLPINDNDRLKEGWRLAYQRIKQRIPGAQIVGPSISRYRLLKPFLEWSH
                     DHNVFPDVVAYHEYDDPSHAPALVEDLRSYLRGRGIVRPISVNEILGQESWTLAGYTV
                     GVIAAYERANILSAMRSCWPDPQDMSRDRVENTCDNPTLDGLLYVDRQSKRPGWHIYK
                     AYAEMDGVRLATMTDSPKLHALASFDKASGTLQLLLGKYEDYSTQDTTVMLKNAARVF
                     SGGQGKNLFLCAEQIPDTGSGPLASTVVTMDRTLPLPDNDISFTLPQFYHSDAYRVRI
                     SPTSGCKPRD"
     assembly_gap    2198..2297
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2298..3123)
                     /locus_tag="PK589_15200"
     CDS             complement(<2298..3123)
                     /locus_tag="PK589_15200"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNJ21008.1"
                     /translation="MRRVFFVDDAPEILKHLQRTLAPMQSEWTMEFFPSAKSALAAIC
                     QAPCDVVVSDMTMPGMDGAQFLTEVRNVSPQAIRIILSGDQSPVNYLRSASVAHRFLQ
                     KPVDVEVLKANIAQAEALRAVLANPALRTLVSEIKALPSLPSIYQDLMQEMQSPQASL
                     KKASRIVAKDLGMVTKILQLVNSAFFGLRTHVSDPEQAVALLGFETIKSLVLSSQVFA
                     QFDQNRLPGFSLDALWRHAMLTATCARRIAKDTGASQPVIDEAFTAALLHDVGVLVL"
     gene            complement(3266..3700)
                     /gene="gspG"
                     /locus_tag="PK589_15205"
     CDS             complement(3266..3700)
                     /gene="gspG"
                     /locus_tag="PK589_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNJ21009.1"
                     /translation="MNASDGFSFIEVMIVVVILAILATLLIPRVMGRTEDAKRAAAKA
                     QISNIESALQLYKLDNGNLPSTEQGLKALVERPGSGPPAPNWKAGGYLPKVPVDPWGT
                     PYKYTVPSSQGAEFEILSFGADGTPGGDGKNADIVSWDLDRN"
     gene            complement(3959..5737)
                     /locus_tag="PK589_15210"
     CDS             complement(3959..5737)
                     /locus_tag="PK589_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21010.1"
                     /translation="MPVDVSLQEILSRPGTLAFPSNLADAPRLPASSASCLRLPTGHL
                     VVYGSHGRRILATDPDGYPLHECEWESVNGRLRLVRSRVHLDWGAWVGLIPGGLVNTM
                     TLDLSKKPGWQRLRADDLRSMAAQAMHVPLDEVRFFYSDQDMTIDPKGRATIRHRKDA
                     IYHLPDGTFDTEQFMACMGAMHWEAIDFLPVVELFLSLLPGTGSALFELIRGLYDDQN
                     RGQVAPRPLRYRGIPTYPSEAAYRLFSNFFSPHLTDGSDPFPVFMDTPRSHLVTWTPV
                     PDPPRRHVDLSQKLCVTIQGRRMLKATRWDDPAGLSYQPFDQRGLAPCQRGLMVRQGQ
                     LLLTDGDMTQAIALPEGWGRVDEPAGAGAALPAREWKTVFGGNPPHVDPGAAFGAVLL
                     YPNDEREIGELATQPFVADYLQDLIEQDRPLAAKVSHAEHVLISGLDAAITTCIGSDR
                     ARAYTVLYDHAAFAQRQAQMWWNLLVRAQRLEWLTRVQMRPRGAEGERRELYDLAYEW
                     TPFSLYDKPESIHHRVQQLASHLSLGAVAFIIGPPTVADSCRTAGVQLQSITPVASLP
                     TFRMHQSVLPRAQLKPGIMLYQIARS"
     gene            complement(5738..7051)
                     /locus_tag="PK589_15215"
     CDS             complement(5738..7051)
                     /locus_tag="PK589_15215"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNJ21011.1"
                     /translation="MEQDLTESPAPELEAPVEVLSPEDYVMGLVKSAKKAAGRLSTLS
                     AAVKNEALEAMAEGLVAHEAELLAANEKDLEQFDATPERKALGDRLRLTAERIRDMAA
                     GLRDVARLPDPLGEMPKMWTRPNGMQVGRMRVPIGVIGIIYEARPNVTADSAALCLKS
                     GNVCLLRGGSEALHSNTAIAKVLSESAEKAGVPAGAITFVDRPEREIVQLLLKQDQYI
                     DLIIPRGGDSLMKTIAEHATIPVLKHDKGVCHTYVDADADPKVAEHICMNAKVQRPST
                     CNAMETLLVHHSIARTWLPHLIKQLTAAKVEVRGCAKTCQLCPEVKPAVEDDFGHEFL
                     DLILAVKVVKHMDEALEHIATFGSRHTEAIVTKDYSRAMRFLREVDAGAVLVNASTRL
                     NDGYQFGLGAEIGISTSRLHARGPMGLEELTCSKFVVLGSGQIRE"
     gene            complement(7174..7860)
                     /locus_tag="PK589_15220"
     CDS             complement(7174..7860)
                     /locus_tag="PK589_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21012.1"
                     /translation="MWDQKSPRSFRLRMIPAQGGNPTKPAMGLRGPLRNARGITYLAL
                     MFSIVLIGISVSAAARQWTVMVQREKEADLMAKGIEIQNALALYSASMKIGRIMTTEI
                     YPQSLAELTRTPKPFLRRVYQDPISGGDWEYMRAPTGGIMGVRSKSKVKPIKERDFPL
                     AVRHFEGRKTYHDWMFQHPNPSSQSMLMPPMMGLAPGTMPTHPGAQGAVPGGGTPQGA
                     PLMPGAPRNP"
     gene            complement(7790..8503)
                     /locus_tag="PK589_15225"
     CDS             complement(7790..8503)
                     /locus_tag="PK589_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21013.1"
                     /translation="MNRVSKNQGMLLLTLVVFWIGLLIWQFDHSNEQARVPLTHVSGQ
                     RATSKTPQAASSGNLQVHLERLAAIKGQREATFSTPRNIFASLLPQVEPVAVPPPAPR
                     RGRSRGPAKAAPPPVLEPLGEPQEQTAAEEEQEPVVEGPKGPTPEEIERLRIATELNQ
                     YRYVGFMAVGGGSQKKKNMAVVVKNDEMHVVQVGEVIGGEVLVKDVSLKEITLQDVGS
                     KITQVIPVTDDPGAGGQPN"
     gene            complement(8509..9078)
                     /gene="pilO"
                     /locus_tag="PK589_15230"
     CDS             complement(8509..9078)
                     /gene="pilO"
                     /locus_tag="PK589_15230"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNJ21014.1"
                     /translation="MFPGLDRLQLTGRQPYAPILPWVAFVVVLAIVSYGIHALVLGAA
                     EEEQVRLEAESVKARQQLNRHREAKKAKADLQRVWAVLPLFRDFAPLALGVTEEAKRD
                     QVTLPALSYKTEKTSVPDATKAVLQGTVTGRYEDLRRFLYNLESAQELLFIEDLNLVR
                     SGNIQDQQLTFNIRISTYLRGEHLQSPTP"
     gene            complement(9081..9704)
                     /locus_tag="PK589_15235"
     CDS             complement(9081..9704)
                     /locus_tag="PK589_15235"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNJ21015.1"
                     /translation="MMLDNLASVLRDVASRIGQPSARSGVFTINLSSRYRWYLAPARL
                     LIMVLAGVIGVAILWDISQSWLVWQDVQARESALLQVQERDNELLKEAQQEGLDLSEA
                     ALQLLPKEIEFANHLIEKRSFSWTRFLTELERAIPQRIAVNSIRLDPANATIMLTGSA
                     RALEDVTTLTVMFQDHPQFKDPVLGQHRVMGNGLVEFDLTLRYRNRS"
     gene            complement(9708..10715)
                     /locus_tag="PK589_15240"
     CDS             complement(9708..10715)
                     /locus_tag="PK589_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21016.1"
                     /translation="MWDWITSRPQYCLKIGAHDLAWAEVHRNWRGRPRYQCVVSALPD
                     GILRLSPLEQNVVSPHELESHIRAIAGSATTQAPGRSSTPGVPRAATVILPDLAVRLA
                     VVRLQDLPWSSDERDALVRWRLGQEQLLSLSGAKVFSQVLSEPGVSGDGPCTVLAVVV
                     QEAVLSQYEAVCEAAGLIPQEVDVASLRLFNLWSHGAWRTAGSTADFLWVNATEGGVT
                     AFIFHKGVLVFLRSKLQGGVGSSGIWPAGSEQLGINRIVQECADSIYACQQQTPELAI
                     SQTILIADDALGPGLRQQLEKGLGVPVQELDWERVQQFGGGPIAGPRTSAAMPAIAGV
                     V"
     gene            complement(10719..12452)
                     /locus_tag="PK589_15245"
     CDS             complement(10719..12452)
                     /locus_tag="PK589_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNJ21017.1"
                     /translation="MAMQRHLTRPSLADVLVREGILPRRTVDQVVARLGGSTTALGHT
                     LVEDGTISEMQLAQALAAQYGLPYDALTGFRVDSEFYHTISVKLMRRHPFVPLKDEAG
                     ILTIAISDPQNLLGLDELEILLNRPLHYVVSSKNAIQAALERSEGSSQALRELEAEYR
                     SVLVKEDERGEEVLTVDHFGEDQSPVVKLLDTIMLSAMQRRASDIHIEATDRATTVKF
                     RVDGILVSAMDPLDVKLHPPLVSRLKVMSDLDIAERRVPQDGSFRMRLDRKTVDFRVS
                     ILPSVFGESVVIRILDREAITTGVSTLRLDRLGFNPEDLKRFRRAITRPYGMVLVTGP
                     TGSGKTTTLYAAISEMNIQEDKLITIEDPVEYQLPGVVQIPVNEKKGLTFARGLRSIL
                     RHDPDKIMVGEIRDAETAQIAIQSALTGHLVLTTVHANNVFDVIGRFASMGIDSYNFL
                     AALTCVLAQRLIRVICPDCRHQVTLDQALAEESGLDYDEFKTSPFYEGKGCSECHDTG
                     YRGRKCITEFLDLTDEIKEMILADRALSEIRYRAVTDGMITLRQSAVKKVLAGETTLR
                     EINRVTFSEER"
     gene            complement(12516..13730)
                     /locus_tag="PK589_15250"
     CDS             complement(12516..13730)
                     /locus_tag="PK589_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNJ21018.1"
                     /translation="MAVFSYRAARADGTTFEGHIEGDDEHVVRAKLEADGVLVFRLSR
                     SGTGFGVPGLSASRWGKLPLQDFLVFNQELLALIKAGLPVLRVWDLLIDRTQRAPFRE
                     ALKAVRQDIRGGASASEALAKHSTYFSDLYLATIRAGEQSGNLAEVLQRYIAYLKLMI
                     SLRQKVTKALAYPGFLVVVGVAVVGFLLSYVMPTFISVYGESSANLPTPTRLLISVIH
                     MGEAQLIPVAIIVIVATVMGRAWYQTSAGRLSVDRNLLRLPLLGTILVEHHTIQLTRT
                     LATVLAGGTPIVEALEIARGAVSNRFVSRGLVSAVNEIREGSTLAAAIERPKILPKLA
                     IEMLSVGEETGSLEPMLRDVAEFYEGDLDVRLSQLTTWIEPVLLLVMGLLVGGIVIIM
                     YLPIFQMAGTIQ"
     gene            complement(13773..14603)
                     /locus_tag="PK589_15255"
     CDS             complement(13773..14603)
                     /locus_tag="PK589_15255"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNJ21019.1"
                     /translation="MIQPHVPARRKGVGLRLGCWLAALLLCESWGGCAKVTPNHEPAP
                     SDLQVAADTLKVAVREAQRTAAELRTELEEQRKELADAQVARAQLQGMLRETERRLTE
                     ARQIIELQREELTSARDERERLAQAVHPFTGHVKASSAPAPSRSKRSPAAQGAAIPGS
                     VDTREMRDDNSLVEQEPAAPQPLPLHREATDSSTAQASAVRVDPLPNRKAVGRTIVVQ
                     GGDTLWGLARRHKVEVAVLRALNGLSGDRILSGRKLRLPESEAQHAAVQPAAVDVVVQ
                     "
     gene            complement(14617..15024)
                     /locus_tag="PK589_15260"
     CDS             complement(14617..15024)
                     /locus_tag="PK589_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNJ21020.1"
                     /translation="MVRNTESGRRAWVSSSAHGFTIIELMIVVTIVGILATLAVPSYH
                     AAIIKAKEGALRQDLFSLRDVIDQHRADKGKYPESIQALVSAGYLRRVPTDPLTGATT
                     SWQEMVDEAEGGMVDVFSGSELVGTNGVPYNQW"
     gene            complement(14999..15505)
                     /locus_tag="PK589_15265"
     CDS             complement(14999..15505)
                     /locus_tag="PK589_15265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNJ21021.1"
                     /translation="MKQSGVTLLELLVTLTILTILASVALPFTKVSTKRTKEIELRQN
                     LRVIRAAIDAFRLEWARDGDTLIGPACVKNRLSCKDVTGPYGYPKSLDALLGVKLTGE
                     QATVRGTTIRRYLRSIPMDPMTGAADWRLRCYRDPASVKDWCGEDIYDVTTQSQELAL
                     DGTKYREW"
     gene            complement(15502..>17967)
                     /locus_tag="PK589_15270"
     CDS             complement(15502..>17967)
                     /locus_tag="PK589_15270"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNJ21022.1"
                     /translation="CGIVQSEKLIVACVSVWYTKEVVTLLIRYMQSPFPSTRVIIEGN
                     RLRRLSMVVSWWACCLALTACGTFASPDVKRGDQHLAAGNWEEASVAYRQALKDDPFE
                     PSLQNKYRISRERAAAAHDERGRQLLKDRQFDQAAEEFKRALTIEPTSKEYEAGLTEA
                     LRLKESRDRYREAERLAQLGRVTEAMTGYMRAVELDPTYKDALEGVARLSAEQHAADR
                     DDRQKQPVTLQFRNAGLKEVVEALGKAAHVNFVFDKDVRNDPITVSLEEKPFDEALSL
                     VLNSNSLFAQKAGPTLFIISPNTKQKQEQYQDLMIRTFYLSSAKAKDMVALLKSMLDV
                     KHIHGNEALNTIVIRDQPEKVELAEKIIQANDREDSEVLFDVEVLEVNRTVDQQYGLN
                     YPKQIGAGLVPPGFTGTIAGDLAQQFTYRNLASLGQDSYLFKVPTNVQLDFFKQITDA
                     KTLAAPKVRVLNNKKAEVNIGDKQPILLSTTNVLPGQAATGAVPTTSTVTSIEFRDTG
                     VKLTVEPNIHLANELSLKMKIEVIRLGDTVLLQQSPPITQFKFGNRSAETMLNVRDGE
                     TIILGGLLQEEDRRTKVTIPWIGDIPYLGNLLSSFKTQRVTTEVILTITPHIVNSLRI
                     PGPQGQAFWSGTESVYSTSPLFAMQPKPISARVAVPAAAGSAIEKSGSKKSKMNAASS
                     DAVSPRLQLSMQPADATVQMEKEIRVDVVATQAQGFDTETFTLEFDPKILEFRDATLG
                     EVLGTEAGKAPVAATSSSTEGIVELRLHRSSTATKDEGRLLRLTFLAKSPGVSPVRLQ
                     MAKHPGQDLPEGTGEASGVVRVR"
BASE COUNT         3821 a         5484 c         4901 g         3661 t
ORIGIN      
        1 cttcaaagag ccacatccac agacccagct ctacggcata cagagtccag ggaaggagga
       61 gaaaccagag caacgccttt cgccgtgcaa gggcgaaggt cctggttgca ccacaataac
      121 tcacgaccac ggaccagaac gacagcaaca cgaagatgag cgtccaactc ccctccgcat
      181 ccctcgccag acggtaattg ggaaacagca gcgtttcgcc tatcatgacg agaacaactg
      241 gaagggcata caccagcgaa gaataaacga acaggagacg tggccggagt gaatccaacg
      301 ccgtggctcc agaccacttg agacgaaggc ggtaccacca cccgcccaga taccagaaca
      361 gaggcacatt cagagcccca cacacgagaa gcgcgatcca aagcgtgccc catgaatgga
      421 gcagccacgg actcaactcc gaccatccgg agactccctg ccccatttct gcacggagaa
      481 tgtgctgctc gattcggtct accatggaag caacacccaa acacagcgtg gccacaagca
      541 gcaccaccgg ccgtgcgaga tcgtcgaacg tcgcaaaaaa gtgtgccggt ttgatgaaga
      601 gagccaccgc gcgaagcagc caggatcgaa cggcattctg aggggttggc ggctggccag
      661 cctgttgatt ggttggcgta tcggtacaca ctgcggcctg gtcttgcata aggagggata
      721 ccttttcttc gctggtcgtg gactggactc gtccatcgat acgcaacaat ccggcacagc
      781 gccgaattac gctcgcccgc ctatcgatgg ttggagggag cgtacagcgg gatgccgtgc
      841 ggccacaatt gtccaggttg gggaaggggc tgctcgaagg agggggacga acctagccct
      901 gaggctttga cggaaagcag cgtttgagag atgccgccgg ggtcgatatc cgttggaaat
      961 actctgacac cacgccggtg gagtggttaa tcgcgaggct tgcacccgga tgtgggcgat
     1021 atcctcaccc tgtaggcatc cgaatggtaa aactgaggca aggtgaagga gatgtcgtta
     1081 tccggaagcg gcaacgtacg atccatcgtc accacggtcg aggcgagcgg gccggatccc
     1141 gtgtccggaa tctgctcggc acagagaaag agattctttc cttgcccgcc tgagaagacc
     1201 cgggcggcat ttttgagcat cacagtcgtg tcctgggttg aatagtcctc gtacttgcct
     1261 aataacaact ggagggtacc ggaagcttta tcaaatgacg ccagcgcatg gagttttgga
     1321 ctgtcggtca tcgtagcgag tcgtacccca tccatttccg cataggcttt gtagatgtgc
     1381 catcccggcc gcttcgattg ccgatcgaca tagagcagac catccagcgt aggattgtca
     1441 caagtatttt ccactcgatc gcggctcata tcttgcggat ccggccaaca ggatcgcatg
     1501 gcactcagga tattcgcgcg ctcatatgcc gcaatgacgc cgacggtgta cccagcaaga
     1561 gtccagctct cctgccccag aatctcattc acagaaatag gtctgacgat acctcgacct
     1621 ctgaggtaac tccgtaaatc ctccacgagc gcaggagcgt gtgacgggtc gtcatactcg
     1681 tggtatgcca caacatccgg aaacacgttg tggtcgtgac tccattcgag aaaaggcttc
     1741 agcagtcggt accggctgat actgggcccc acaatctgcg caccgggaat ccgttgttta
     1801 atacgttgat aggccagccg ccagccttct ttgagccgat cgttgtcatt gataggaagc
     1861 gtggctaaat cgggttcatt atagatatcc cacaaaacgg agaggcgacg atccatcact
     1921 tgatcgatga ttcgatccac atgcgcgagc cagggtgcgt agtttccatc cactccaatt
     1981 tgattgatat caaaaatttg cagaggcagg tctgcgaaca ccagcccaag ggtaagctga
     2041 aactccgtta ccccggcctg cttgagccga tcataattct tgaagacgta cccggcattg
     2101 cctctaaaac tcgtgggctt cagcggaagg atgaattcca ttttcggctc gtcgggctcg
     2161 atgctcacca ggtacccgtt cgcccggtgg ttcatcgnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnncca acaccagcac gcctacgtcg tgcagcagcg ccgcggtaaa
     2341 cgcttcgtca atgaccggct ggcttgcacc ggtgtctttg gcaatccggc gggcacaggt
     2401 tgccgtcagc atcgcgtgcc gccacagcgc gtccagtgaa aatcccggca accgattctg
     2461 gtcaaattgc gcaaagacct ggctggagag cacgagcgat ttgatcgtct caaatcccag
     2521 aagcgccacc gcctgttcag gatcagacac atgcgtgcgg aggccgaaaa atgccgaatt
     2581 gaccaactga aggatcttcg tgaccatgcc gagatccttg gccacgatgc gagaggcttt
     2641 cttcaatgag gcctgcgggc tctgcatctc ctgcatcaag tcctgataga tcgacggcag
     2701 actcgggaga gccttgattt cactgacgag cgtccggaga gcaggattgg ccaacaccgc
     2761 ccgcaaggcc tccgcctgcg caatgttcgc cttcaggacc tccacatcca ccggcttttg
     2821 cagaaaccga tgcgcgaccg aggccgagcg cagatagttc accggactct gatcgccgga
     2881 gagaataatg cggatcgctt gtggagaaac gttgcgcacc tcggtgagga attgggcacc
     2941 gtccatcccc ggcatggtca tgtccgacac cacgacgtca caaggtgcct gacagatcgc
     3001 agctaacgcg ctcttggcgg agggaaaaaa ctccatcgtc cattcggact gcatgggggc
     3061 caacgtccgt tgcaggtgtt tgagaatttc cggcgcatcg tcgacaaaaa agacgcgtcg
     3121 catgggccct cctcccggca ggattcgtct cttcttctca tcggtcgaga ggggcaggat
     3181 cttaagtcag attgaaccac tacaacgggg agatgaggca atcctgtgga ttggaaagag
     3241 aactcagcgg gcctgactcc agacactagt tgcggtccag atcccaactg acgatatcgg
     3301 cgtttttgcc gtcgcctccg ggagtgccat ccgcgccgaa cgagagaatc tcgaattcgg
     3361 ccccttgcga agacggcacc gtgtatttgt agggggtgcc ccacggatcg accggcacct
     3421 tcggaagata cccacccgcc ttccaattcg gcgcaggggg acctgatccg ggccgttcca
     3481 ccaacgcctt caggccctgc tcagtcgacg ggaggttgcc gttatccagt ttgtacaatt
     3541 ggagcgcgct ttcgatgttt gaaatttgtg cctttgcggc cgcccgcttg gcatcttccg
     3601 tccggcccat cactcgagga atcagcagcg tcgcaaggat cgccaggatc acgacaacga
     3661 tcattacctc gatgaacgaa aatccgtcac tcgcgttcag tcttcctgca agacctgagc
     3721 aacgatgctc gcacgatgca ccgcggtcta gccctgtaga cttcctgtat tctcgaaaaa
     3781 accccatacg tgctgctcct tctctcaacc gatccggctc gctgcgagct ccatgcgtgg
     3841 accaattttg aattgtaaca ggaacgtcta tgagtgtgtc attcaaatcc gaggtgacat
     3901 gctcctgtca gcgctctcgt acgcgtctct atacacgcat ctgccatgag gccaccgatc
     3961 aggagcgagc gatctgatat agcataatgc cgggcttgag ttgcgcgcgg ggtaacacac
     4021 tttgatgcat gcggaacgta ggcagagacg ccacgggcgt gatcgactgc agctgcaccc
     4081 cagccgttcg gcaactgtct gctacggtcg gcgggccgat gatgaatgcc accgccccaa
     4141 gcgacaggtg gctagccaat tgctgcacac gatggtgaat cgactccggc ttgtcataca
     4201 gactgaacgg agtccattca tatgccaggt cgtacaactc ccggcgttcc ccttccgctc
     4261 cacgaggccg catctgcaca cgcgtgagcc actctagtct ctgcgcccga accaggaggt
     4321 tccaccacat ctgcgcctga cgctgtgcaa acgccgcatg gtcatacaac actgtatagg
     4381 cccgcgcacg atcgctcccg atacacgtgg tgatagccgc atcgagtccg gaaatcagca
     4441 catgctccgc atgggatacc ttcgcagcca atggccgatc ttgctcgatg agatcttgaa
     4501 ggtagtcggc gacaaacggt tgcgtcgcca attccccaat ttcccgctca tcgttgggat
     4561 agagcaatac ggcaccaaac gccgctcccg gatcgacatg cggcgggttc ccaccgaaca
     4621 ccgttttcca ctcacgggcg ggcaaggccg cacctgcccc ggcaggttcg tcgacgcgtc
     4681 cccaaccctc gggcaatgca atcgcctgcg tcatgtctcc atcggtcaac aggagctgcc
     4741 cctgccggac catcaatccg cgctggcagg gtgcgaggcc tctctgatcg aagggctgat
     4801 aggaaagccc tgccggatca tcccaccggg ttgctttcag cattcgccga ccttgaatcg
     4861 tgacgcacaa tttctgggac aggtcgacat gtcttctcgg cgggtccggg accggggtcc
     4921 atgtcaccag gtgggatcgc ggtgtatcca tgaacaccgg aaagggatca ctgccgtccg
     4981 tcaggtgagg agaaaagaaa ttactgaaca gacgataggc tgcttctgac ggataggtcg
     5041 gaataccacg gtatcgcaac ggccttggag caacttgtcc gcgattctga tcatcataga
     5101 ggccacggat caattcaaag agtgcggagc cagtgccggg cagcagcgat aaaaagagct
     5161 cgacgaccgg cagaaaatcg atggcttccc aatgcatggc ccccatgcac gccataaatt
     5221 gttcggtgtc gaaggtccca tcaggcagat ggtagatggc atctttgcga tgccggatcg
     5281 tggcgcgacc cttgggatca atcgtcatgt cctgatcgct gtaaaaaaac cggacttcat
     5341 cgagcgggac atgcatggct tgagccgcca tgcttcgaag atcgtcggcc ctgagccgct
     5401 gccatccagg cttcttggac agatccaggg tcatcgtatt gacgagcccg ccgggaatga
     5461 gtccaaccca tgccccccaa tccaggtgta cccggcttcg aacgagacgc agccggccat
     5521 tgaccgactc ccattcgcac tcatgcaatg gatacccgtc cggatccgtc gctaggattc
     5581 gccgcccatg gcttccatag acgacgagat gtcccgtggg cagacgaagg cacgacgctg
     5641 atgacgccgg caagcgcggc gcatcagcaa gattggaagg aaaggcgagc gtaccggggc
     5701 gactcagtat ctcctgtagc gagacatcca caggcattta ttcgcggatc tgcccgctcc
     5761 caagcaccac gaacttggaa caggtcaatt cttccagccc catcggccct cgcgcgtgaa
     5821 gccgcgaggt gctgatgccg atttctgctc cgaggccgaa ctgatacccg tcattgagcc
     5881 tggtcgacgc gttgaccaaa actgcacccg catcgacttc gcgcaagaaa cgcatggcgc
     5941 gggaataatc cttggtcaca atcgcttccg tatgacggga gccgaatgtg gcaatgtgct
     6001 ccagcgcttc atccatatgt ttgaccactt tgaccgcgag aatgagatca agaaattcat
     6061 gcccgaaatc atcctccact gccggcttga cctcaggaca gagctggcag gtctttgcgc
     6121 aaccacgcac ttccaccttc gcagccgtga gctgtttgat gagatgcgga agccaggtcc
     6181 gtgcgatgct gtggtggacc aacagcgtct ccatggcatt gcacgttgag ggtcgctgca
     6241 ccttggcatt catgcagatg tgttcagcca ctttcgggtc ggcatccgca tcaacatacg
     6301 tgtgacagac gcccttatca tgcttcagca ccggaatcgt cgcatgttca gcaatggtct
     6361 tcatcaacga atccccgccc ctggggatga tgaggtcgat atactgatcc tgcttcaaca
     6421 acagctggac gatttcacgc tccggccgat ctacaaaggt gatggcgccg gccggcacac
     6481 cggccttctc tgctgattcg ctcaacactt tggcaatcgc cgtattggaa tgcagcgctt
     6541 ccgatccgcc gcgtaggaga cagacatttc ccgacttgag acagagagcg gccgagtccg
     6601 ccgtgacgtt cggacgagcc tcataaatga ttccgatgac cccgatggga acccgcatcc
     6661 ggcccacctg catgccgttc ggccgcgtcc acatcttggg catctcgcca agcggatccg
     6721 gcaggcgggc aacatcacgc aagccagcag ccatatcgcg aatacgctcc gccgtcagtc
     6781 gcaaccgatc acctagcgcc ttgcgctcgg gcgtggcatc gaattgttcg agatcctttt
     6841 cgttcgccgc cagcagctcc gcctcatggg ccacaagccc ttcagccata gcctccaagg
     6901 cttcattttt gactgcagcc gaaagggtcg acagccggcc cgccgccttc ttggcgctct
     6961 tcaccaaacc catcacatag tcttcaggag acagtacttc aacgggagct tccagctccg
     7021 gagccgggga ttcggtcagg tcttgttcca tgtcgtgaat tcctgctgct tggcgataca
     7081 gaggcccgag gccaaaggta tccaccgatc aagatggtga acgtaggcga aaaaacaata
     7141 gcgtgacagg atactgtggg gtttggagtc gggtcaaggg ttacggggag cccctggcat
     7201 cagaggagcg ccttgtggtg tcccgccgcc gggaacggcc ccttgtgcac cagggtgtgt
     7261 cggcatggtc ccgggcgcca gccccatcat gggcggcatc agcatggatt gggaagacgg
     7321 attgggatgc tggaacatcc agtcatggta ggtcttgcgc ccttcgaaat ggcggacggc
     7381 cagcggaaaa tcacgctcct tgatcggttt gactttactc ttgctgcgca cccccatgat
     7441 gcccccggtg ggcgctcgca tgtactccca gtctcccccg ctgatcgggt cctgatagac
     7501 tcgtcgcaaa aatggtttgg gcgtgcgcgt caattcggct agagactgag gatagatttc
     7561 ggtcgtcatg atgcgcccga ttttcatcga ggccgagtac aacgccagcg cgttttgaat
     7621 ttcgatccct tttgccatga gatcggcttc tttttcgcgc tggaccatca ccgtccactg
     7681 gcgagccgcg gcagaaacgg aaatgccgat caagacgatc gaaaacatca aggccagata
     7741 ggtgataccc cgggcatttc ggagaggacc ccgcaacccc atcgccggct tagttgggtt
     7801 gcccccctgc gccgggatca tccgtaaccg gaatgacctg ggtgattttt gatcccacat
     7861 cttgaagcgt aatttccttc aacgacacat ctttgaccag gacttctccc ccgatcacct
     7921 ctcccacctg cacaacatgc atctcgtcat tcttgaccac caccgccatg ttcttctttt
     7981 tctgcgatcc tcctccaacc gccatgaagc cgacatagcg gtactggttg agctcggtcg
     8041 cgatccggag ccgttcaatt tcttccggcg tcggcccctt tggcccttct accacgggtt
     8101 cctgctcttc ttcagcggca gtctgttcct gtggctctcc gagcggctca agtacgggag
     8161 gaggagctgc cttagcaggc ccacgtgaac ggcctcggcg gggagccgga ggcggaaccg
     8221 ctaccggctc cacctggggc agcaggctgg cgaaaatgtt gcgcggcgtg gaaaacgtgg
     8281 cttcgcgctg gcccttgatg gcggccaagc gctccaaatg aacttgtaaa ttaccactgg
     8341 aagcggcttg aggcgtctta ctcgtggctc gctgtccact gacatgggtc aacgggactc
     8401 gcgcctgttc gttcgaatgg tcaaattgcc agatgagcaa cccgatccag aagacgacga
     8461 gggtcagcaa cagcatcccc tgatttttac tgactcggtt catgagcgtc agggtgtcgg
     8521 agattgcaga tgctctcccc gcaaataagt cgaaatcctg atattgaacg tcaattgctg
     8581 gtcctgaatg tttcctgagc ggacgagatt gagatcctcg ataaacaata gttcctgggc
     8641 cgactccaag ttgtagagga agcggcgcaa gtcctcatac cgcccggtca ccgtcccctg
     8701 cagcaccgcc ttggtggcat ccggtaccga cgtcttctct gtcttatagg acagagccgg
     8761 cagcgtgacc tgatcccgct tggcttcttc cgttacaccg agggcgagcg gggcaaaatc
     8821 ccgaaacaac ggcaggacgg cccacacgcg ctgaagatcc gccttcgcct tcttggcttc
     8881 cctgtgccgg ttcaactgct gccgcgcctt gaccgactcg gcttcgagcc gcacctgctc
     8941 ctcctccgcc gcacccaaca ccagggcgtg aatgccgtaa ctcacgatgg ccaggaccac
     9001 cacgaaagcc acccagggaa ggatgggcgc gtagggttga cgcccggtca attgtaggcg
     9061 atcgaggcct ggaaacatcg ttagctccgg tttcgatacc gcaacgtgag gtcgaactcg
     9121 accagcccgt ttcccatcac ccggtgctgg cccaacacgg gatccttaaa ttggggatgg
     9181 tcttgaaaca tcaccgtcag cgtggtcaca tcttccagcg ccctcgccga tccggtcagc
     9241 atgatcgtcg cattcgcggg atcgagacga atactgttga cggcaatgcg ctgaggaatc
     9301 gcacgctcca gttccgtcag gaaccgggtc catgaaaagc tccgcttctc gatcagatga
     9361 ttggcgaact cgatctcttt cggcagcaat tgcagggcgg cttcggaaag gtccaggcct
     9421 tcctgctgcg cctccttgag caattcgttg tcccgctcct ggacttgcaa cagggctgat
     9481 tcgcgcgctt gcacgtcttg ccacaccagc caagattgag aaatgtccca aaggatggcc
     9541 acgccgatca ctcccgccaa taccataatc agcaaccgtg ccggcgccag ataccaacga
     9601 tagcggctgc tcagattgat cgtgaataca ccgctgcggg cagacggttg accgatacgc
     9661 gaggccacat cgcgaagcac cgatgccaga ttatccaaca tcatgactta cacaacccct
     9721 gcgatcgccg gcatggccgc gcttgtccgt ggcccggcaa tcggaccacc gccgaattgt
     9781 tgcacccgtt cccaatccaa ttcctgcacg ggcacgccta atcctttttc caattgctgg
     9841 cgcaagcccg gtcccagggc gtcatctgca attagaatgg tttggctgat cgctaattca
     9901 ggcgtctgtt gctgacaggc gtaaatcgag tcggcgcact cctgcacgat ccggttgatc
     9961 cccaactgct ctgagccggc aggccagatc ccactggatc cgacgccccc ctgcaatttg
    10021 gaacggagga agaccaagac gcccttatga aagataaatg ccgtgacccc gccctcggtg
    10081 gcattcaccc acaaaaaatc ggcagtcgag cccgcggtac gccaggctcc atgggaccac
    10141 aggttgaaca ggcgcaggct ggcgacatcc acttcctgcg ggataagacc ggcggcttcg
    10201 cacacggctt catactgcga cagcacggcc tcctgcacca ccaccgcgag gacggtgcag
    10261 ggcccgtcac cggacacgcc gggttcggac aacacttggg agaagacttt ggccccgctt
    10321 aaggacagca actgctcctg ccctaatcgc caacgcacca acgcatcccg ctcatccgaa
    10381 ctccacggaa ggtcttgcaa gcggacaacc gccagacgca cggccagatc cggcagaatc
    10441 accgtggcgg cgcgcgggac tcccggggtg gaagaacggc caggagcttg ggtcgtcgcc
    10501 gacccggcaa tcgcgcggat atgcgattcc agttcatgcg gagacacgac gttctgttcc
    10561 aatggcgata gccggaggat gccatctggc aacgcggaca ccacgcattg ataccgtgga
    10621 cgcccgcgcc aattgcgatg cacctccgcc caggccagat catgcgcgcc gattttcaag
    10681 caatactgcg gtctgctggt aatccaatcc cacatcgtct atcgctcttc actgaacgtc
    10741 acacggttga tctcgcgcaa cgtcgtctcc ccggccagca ctttcttgac cgcagattga
    10801 cgcagcgtaa tcatgccgtc cgtgaccgcc cggtaccgga tctccgacaa cgcacggtca
    10861 gccagaatca tttccttgat ctcgtctgtc aggtccagaa actcggtaat gcacttgcgg
    10921 ccacgatacc ccgtgtcatg acactcggag cagcctttgc cttcatagaa cggcgaggtc
    10981 ttgaattcgt cataatcgag accggactct tccgccagcg cttgatccag cgtgacctga
    11041 tggcgacagt ccggacaaat cacccggatc aatcgttgcg ccaacacgca ggtcaacgcc
    11101 gcaaggaaat tataggaatc gatgcccatc gaagcgaacc gcccgatcac atcgaacaca
    11161 ttgttggcgt gcaccgtggt caacaccaaa tggccggtga gcgccgattg aatggcgatc
    11221 tgcgcggtct ccgcatcacg gatttctccg accatgatct tgtcaggatc gtgacgcaga
    11281 atggaccgta acccgcgggc aaaggtgagg cctttcttct cattcaccgg gatttggacg
    11341 acacccggca actgatattc gaccgggtct tcgatggtga tcagcttgtc ttcctgaatg
    11401 ttcatttcgc tgatggcggc gtagagcgtc gtggtcttgc cgcttcccgt cggaccggtc
    11461 acgagcacca tcccgtaggg cctcgtaatc gcgcgccgga accgcttgag atcttcagga
    11521 ttgaatccca gccgatccag ccgcaaggtg gagacgccgg tggtgatcgc ctcgcggtcc
    11581 aaaattctga tcacgaccga ttcgccgaac acgctcggga gaatcgacac gcggaagtcg
    11641 accgttttcc ggtcaaggcg catccggaaa ctcccgtctt gcgggacccg gcgctctgcg
    11701 atgtccagat cggacatgac cttgaggcga gagaccagcg gcgggtgcag cttcacatcc
    11761 agcgggtcca tggctgagac cagaatcccg tccacgcgaa atttcaccgt cgtcgcacga
    11821 tccgtggctt caatgtgaat atcgctcgcc cgccgctgca tggcgcttaa catgatcgtg
    11881 tcgagcagct tgacgacggg gctttggtcc tcgccgaaat ggtctaccgt caggacttct
    11941 tcgccccgct cgtcttcctt gacgagaacc gaccggtact cagcctctaa ttcacgtaag
    12001 gcctgactcg atccttcgct gcgctccagt gcggcctgaa tcgcgttctt tgaactcact
    12061 acatagtgca acgggcgatt gagcaaaatt tccaattcat ccagtccaag caggttctgg
    12121 ggatcggaaa tcgcaatggt gaggatcccc gcctcgtctt tcaggggcac gaagggatga
    12181 cggcgcatga gcttgacgga aatggtgtga taaaactccg aatccacccg gaagccggtc
    12241 agcgcgtcat agggaaggcc atattgcgcg gctaacgctt gcgccaactg catctctgaa
    12301 atggtgccgt cttcaaccag cgtgtgtccc aacgcggtgg tgctgcctcc aaggcgggcc
    12361 acgacctgat cgaccgtgcg gcgaggaagg atcccttcgc gcaccaacac atcggccaac
    12421 gacgggcggg tgagatgacg ttgcatggcc atactacaaa tctccacccc tgatgggact
    12481 ccaggctcct gaacgatgag gtctaccccg ccgcattatt gaatcgttcc ggccatctga
    12541 aaaatgggca agtacatgat gatgacaatt ccgccgacga gcaaccccat gaccagcaac
    12601 aagaccggct cgatccacgt cgtcaactgg ctgagccgca catcgagatc accttcataa
    12661 aattcagcca catccctgag catcggctcg agcgagcctg tctcctcccc gaccgacagc
    12721 atttcgatgg ccagcttggg caaaattttg ggcctctcaa tggccgccgc cagcgtgctg
    12781 ccttcacgga tctcattgac cgcggacacc agtccgcgtg agacgaagcg gttcgagacc
    12841 gctccgcgcg caatttccag cgcttccaca atcggcgtac cgccggccaa caccgtcgcc
    12901 aatgtccgcg tcaattggat cgtatgatgc tccacgagaa tggtcccgag taagggcaac
    12961 cgcaacagat tccggtccac cgataaccgt ccggccgatg tttgatacca agcccgcccc
    13021 atgacggttg ctacaatgac gataatggcc actggaatca attgcgcctc tcccatatga
    13081 atgacggaga tgagcaatcg cgtcggcgtc gggagatttg cggacgattc tccgtacaca
    13141 gagataaagg tcggcatgac gtagctcagg agaaatccga ccaccgcaac gccgaccacg
    13201 acgagaaaac ccggatatgc caaggccttc gtcacctttt ggcgaaggct gatcatcagc
    13261 ttcaagtagg caatgtaccg ttgcaacacc tccgccaaat tccccgactg ttcgccggcg
    13321 cgaatggtgg ccaaatagag atccgaaaaa taggtggagt gtttcgccaa ggcttcggat
    13381 gccgaggccc cgccacgaat atcctgacga acggccttga gcgcttctcg aaacggcgcg
    13441 cgctgcgtgc gatcgatgag aagatcccac acccgaagaa ccgggagccc cgccttgatt
    13501 agggccagca gctcttgatt aaacacgaga aaatcttgaa ggggaagctt gccccagcgg
    13561 ctcgcagaca ggcccggcac accaaaccct gttccgcttc gggagagccg aaagaccagg
    13621 acgccgtctg cttcgagttt cgcgcggacc acatgctcat cgtcgccttc gatgtggcct
    13681 tcgaaggtcg ttccatccgc gcgagccgct ctataggaaa aaacagccat gagttcattc
    13741 ggctcggcac atcacgcgat aatcttcaac cgctattgca ccacgacatc gactgcagcc
    13801 ggttgcacag ccgcatgctg cgcttccgac tcaggcaaac gaagctttcg tccagatagg
    13861 atgcggtcac ctgacaaacc gttgagtgcg cgaagtaccg cgacttcaac cttgtgccgg
    13921 cgcgcgagcc cccataacgt atcgccgcct tgcaccacga tcgtccgacc cacggccttt
    13981 ctgtttggaa gcgggtcgac acgaacggct gatgcctgag ccgtcgatga gtctgtggcc
    14041 tcgcgatgaa gaggcagagg ctgcggagcc gccggttctt gctctacgag ggaattgtca
    14101 tccctcatct cccgcgtgtc gacggagccc ggaatcgccg caccctgggc agcgggtgac
    14161 ctcttgctgc gcgatggagc aggagcagag gatgccttca catgcccggt aaaggggtgg
    14221 accgcctgag ccaatcgttc ccgctcgtcg cgagctgagg tgagctcttc acgctgcaat
    14281 tcgatgatct gacgggcctc cgtcagtcgc cgctctgttt cccgtaacat gccctgtagt
    14341 tgtgcacgcg ccacctgcgc gtcggccagt tcttttctct gttcctccag ttccgtccgc
    14401 aactcagccg ccgtccgctg cgcttcgcga accgcgacct tgagggtgtc cgccgccact
    14461 tgcagatccg agggggctgg ttcgtgattg ggcgtgacct tcgcgcagcc tccccaggac
    14521 tcacagagga gcaatgcggc caaccagcat ccgagacgta acccaactcc cttccgtcgc
    14581 gcaggaacat gtggctgtat catgatgttc attgccttac cattggttgt agggtactcc
    14641 attggttccg accagttcgg agcctgaaaa cacatcgacc atcccgcctt cggcttcatc
    14701 gaccatctcc tgccacgatg tggtcgcccc ggtcaacggg tcggtcggga ctctcctgag
    14761 gtaaccggcc gataccaggg cttgaatgct ctccggatac ttccccttgt cggcgcgatg
    14821 ttggtcgatc acgtcgcgta acgagaacaa atcctgacgc aaggcccctt ccttcgcttt
    14881 aatgatcgcc gcgtgatacg acggcaccgc caatgtggca agaatcccta cgatcgtcac
    14941 cacaatcatc aactcgataa tcgtaaatcc gtgcgctgac gaggagaccc atgcgcgcct
    15001 accactctcg gtatttcgta ccatccaagg ccagttcttg actctgagtc gtaacatcgt
    15061 aaatgtcttc tccgcaccag tctttcacgc tggcggggtc ccggtagcaa cgcaggcgcc
    15121 aatcggctgc gccggtcatc ggatccatgg gaattgagcg cagatagcgc cgtatcgtcg
    15181 ttccccgcac cgtcgcctgc tcaccggtga gtttgacccc cagcaaagca tcgagcgact
    15241 tcggatatcc gtaggggccg gtaacgtctt tgcaactcaa ccgattcttg acgcaggccg
    15301 gaccgataag cgtgtcaccg tcacgagccc attccaggcg aaaggcatca atcgcggctc
    15361 gtatcacccg gagattctgc cgaagttcga tctccttcgt gcgtttcgtg gagaccttcg
    15421 tgaacggcag cgcgaccgag gcaaggatgg tgagaatcgt cagggtcacg agcagctcaa
    15481 gcaacgtcac gccggactgt ttcaccgcac tctcaccact cctgaggctt cgcccgttcc
    15541 ctccggcagg tcttggccgg gatgtttggc catctgcagc cgcaccggcg agacacccgg
    15601 ggatttggcg aggaaggtca atcgcaacag gcggccttca tctttggtgg cagtcgaaga
    15661 gcgatgcagc cgcagctcca caatgccctc cgtggaagat gatgtcgcgg ccaccggagc
    15721 tttgccggcc tcggttccca acacttcacc gagggtcgcg tctcgaaatt cgagaatctt
    15781 cggatcaaac tctagcgtga aggtctccgt atcgaaccct tgggcctgag tcgccaccac
    15841 atccacacgg atctctttct ccatctggac tgtggcgtcc gcaggctgca tagacagctg
    15901 aagtctgggc gagaccgcgt ctgaactcgc ggcattcatt tttgacttct tcgacccgct
    15961 tttctcaatg gctgatccgg ctgccgccgg gaccgccacc cgcgccgaga tcggcttcgg
    16021 ttgcatggca aataatggcg acgtggagta cacggactca gttcctgacc aaaacgcctg
    16081 cccttgcggc ccgggaatgc ggagcgaatt gacgatatgc ggcgtaatgg tcaggattac
    16141 ttcggtcgtc acccgctggg tcttgaagga actcagaaga tttcccagat acggaatgtc
    16201 gccgatccat ggaatggtca ccttggtgcg acgatcctcc tcctggagca agccgccgag
    16261 gatgatggtt tctccatcac gcacgttcag catggtctct gccgagcgat tcccgaactt
    16321 gaactgcgtg atcggtggag attgctgcag caggacggtg tcacccaggc ggatcacctc
    16381 aatcttcatc tttaaggaca gctcgttcgc gagatgaatg ttcggttcca ccgtcagctt
    16441 cacaccggtg tcccgaaact caatcgacgt caccgtcgag gttgtcggca ccgcgccggt
    16501 agcggcttgc cccggcaaca cgttggtggt cgacaacaga atcggttgct tatcgccgat
    16561 gttcacttcc gcctttttat tgttcagcac tcgcactttc ggcgcggcga gcgtcttggc
    16621 atcggtgatt tgcttaaaga aatccaactg cacattcgtg ggaaccttga acagataact
    16681 gtcctgcccc aggctggcca aattgcgata ggtaaactgc tgcgccaggt cgccggcgat
    16741 cgtcccggta aatcctggag gcacgagccc tgcgccaatc tgcttgggat aattcagccc
    16801 gtactgttga tcaaccgtcc ggttgacctc cagcacttcc acgtcgaaca gcacctcgga
    16861 atcctcgcgg tcgttggcct gaatgatttt ttcggctaac tccacctttt ccggttggtc
    16921 acgaatgacg atggtattga gggcctcatt gccatggatg tgtttcacat ccagcatcga
    16981 cttcaggagg gccaccatat ctttcgcctt ggccgacgac agatagaagg tccggatcat
    17041 caggtcctga tactgttcct gcttctgctt ggtgttggga ctaatgatga agagcgtcgg
    17101 gccggccttc tgcgcaaaca ggctgttgct gttcaacacc agcgacaagg cctcgtcgaa
    17161 gggtttttct tcgagcgaga ccgtgatggg atcgtttcgc acatccttat cgaacacgaa
    17221 attgacgtga gcggccttcc ccagcgcctc tacgacttcc ttcagccccg cattgcgaaa
    17281 ttgcagggtc accggctgct tctgccgatc atcacgatcg gccgcatgct gttcggcgga
    17341 cagacgggcg acgccttcga gcgcatcctt ataggtgggg tccaactcga cggctcgcat
    17401 atagccggtc atggcttcgg tgacacgccc tagttgcgct aaccgttctg cttcccgata
    17461 ccggtcacgc gattctttca atcgcagcgc ctcggtcaag cctgcttcat actctttgct
    17521 ggtcggttca atcgtgagag cccgcttgaa ctcctcggcg gcctggtcga attggcgatc
    17581 cttcaataat tgtcggccgc gttcatcatg cgctgcggcg gcacgttctc tcgaaatgcg
    17641 atacttgttt tgtagcgacg gttcgaacgg atcatccttg agtgcctgcc gatacgcaac
    17701 gctcgcctcc tcccaatttc ctgccgcgag atgctggtcc ccgcgtttga catcgggaga
    17761 cgcaaacgtg ccgcaggcag tcagtgccag gcagcatgcc caccagctga cgaccatgga
    17821 cagccggcga agccgattac cttcgatgat gacgcgtgtg gaagggaagg gtgactgcat
    17881 atatctgatc aacaaggtga caacctcttt ggtataccaa actgacacac acgcgacaat
    17941 cagtttctcg ctttggacga tgccgca
//
LOCUS       DAOQDS010000074        17535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1305_length_17535_cov_18.900458, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000074 DAOQDS010000000
VERSION     DAOQDS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17535
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1305_length_17535_cov_18.900458"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK589_15275"
     CDS             complement(<1..215)
                     /locus_tag="PK589_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21023.1"
                     /translation="MDETELMFDSSQTVELTLEADRTAQSARKAILGLWTLRERLPIN
                     LPNRELAHIDFHADRSAQFTFPSSLLG"
     gene            587..1102
                     /locus_tag="PK589_15280"
     CDS             587..1102
                     /locus_tag="PK589_15280"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNJ21024.1"
                     /translation="MVKTLPISEAREQLRALVEQANRTMARVIITRNGKPEAVLMGYA
                     EYESWLETLDVMADHDELAAIEAGLADVRAGRLVSAEDAYREAAPPKDTPLEGPGNIG
                     SGGLTEREVTVLHRIAAGSLENEIAHDLRLAPKTIHALKMSIFRKLGIRTHLEARHDT
                     VQKRKADKVGR"
     gene            1059..1313
                     /locus_tag="PK589_15285"
     CDS             1059..1313
                     /locus_tag="PK589_15285"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNJ21025.1"
                     /translation="MTPFKNVKLTKSAAKELRPIPQKFRAAVQAALDALAADPLLGTA
                     LLPPFEGCRRYRIGNYRIVYQFDATTLIVLSIRHRKDVYR"
     gene            complement(1617..1692)
                     /locus_tag="PK589_15290"
     tRNA            complement(1617..1692)
                     /locus_tag="PK589_15290"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1657..1659),aa:Met,seq:cat)
     gene            1953..2882
                     /locus_tag="PK589_15295"
     CDS             1953..2882
                     /locus_tag="PK589_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNJ21026.1"
                     /translation="MMTGISLDTILAWLITMSAAVGIASLRIGLVLAIGYVAIRFVRL
                     GLHQLERVMIVASDKQDQESGTAAKRAATLTGILRTIALSAIWAIIIIESLQLVGLDI
                     APILAGAGILGLAVGFGAQNLVRDLISGFFIILEDQVRLGDVAVINGTGGAVETITFR
                     TISLRDFSGVVHIFPNGGITTLSNMTKDWSAFVLDMGVAYREDTDRVVEVMRTVGEGL
                     RQDQAFGSLIIEPIEIVGVENFADSAVTIRARIKTKPAEQWKIGREYRRRLKKAFDAQ
                     GIDIPFPMRTLLMGETDQPLKVEVIAGLGLGKS"
     gene            complement(2895..4901)
                     /locus_tag="PK589_15300"
     CDS             complement(2895..4901)
                     /locus_tag="PK589_15300"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ21027.1"
                     /translation="MSTLIKGFERIEEARERLTGASFLAGLFDGRPDFEFLLTPPEPP
                     EERAAGMAFCKQVETFLRQEVDPDEIEREAKIPDAVIRGLFKLGAFGMKIPKAYGGLG
                     FSYTNYGRVLTLIASWSNILALTVAVPQSIGIAMPILLFGNEDQKRRYLPLVATEALS
                     AFALTEPMTGSDAANVRTEATLDANGSHFLVNGEKLWCTNGPIARYATLIARVPARRV
                     VREGQTTWMPVPEGQGADDRVHTAFILDMSTPGMQVRQRCQFEGCRGIENAHMTMQEV
                     RIPVENVIGEVGKGLKYALTILNVGRGISIPAICLGMAKQAWQPTLDRANGRVTFHKP
                     LAERQTQQIRLGDMAAHLFAMEALSMLVWRLADQHRHDIRIEAAVAKIFCSEHTIQFL
                     RDAQILFGGMGYETAASKMARGEAGFGIEQLVRDAEMYRIGEGATDILRPFIVREGLS
                     HHLDRAKPFYSGDLSILDQLKQLVKLGGFYGPWYLRHWVRHSLPTGSAFAHPQVRSVA
                     RYVERTSRRLAREIFYAMVRFQAAFQDEQRVQNRIESIGEDLLAMIATVLYAEQQTRL
                     EGRTTVWELAEAFHVAARKRITQRLREIRRHCDGVTAETGQQALKGYYPGLSDGIVHR
                     RLDDYRRKQAVSEAMPLAMASVSLDVKDRSLFAPVKAKAGERST"
     gene            complement(4928..6646)
                     /locus_tag="PK589_15305"
     CDS             complement(4928..6646)
                     /locus_tag="PK589_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNJ21028.1"
                     /translation="MNLLNDVRDHIEQARDVSGRATDVPWASFAEFLRSRVYDPQLAT
                     THALTFCDDDRALRRTYTYAELGAIIERLAGVFHTRFGLTRGDRVATLLFNHDVTVLT
                     YLAAWTLGVAVVPINIEETPEKKRYILEQSEVSTVFCWQDGYEELCALQSDIPTLRQV
                     IALGDAGVLDNHRTGKKFHAHGAGRSLRRSPFTSGLDDHALIVYTSGTTGPPKGVILT
                     VANLLIDADAIASWHSFGLHDRLMCVLPIHHVNGIVVTLITPLYCKGSVVMNRRFKTT
                     SFWRRIHEEQVTSVSVVPTLLEFLLDADEDLTPYDLSRLGGLICGAGPLLKDTAARFE
                     HRFGVVIRHGYGLSETTCYSCFLPNDLTQEQHRHWLTGYELPSVGVPVRHNLMTVLDE
                     RGRTLPPATRGEICIRGRTVCAGYFKRDDANEAAFQWGWFRSGDEGFYLLDDSGRPFF
                     FVSGRLKELIIRGGVNISPLEIDDVLKTHQAVKFAMALPFENRYYGEEIAAYVVPKDP
                     ASIPSEADVLAHCRMRLPFSKQPKVVLFGDDVPYTSTGKPKRLELKARLAGRLAQYRD
                     TQFRDQ"
     gene            complement(6621..7799)
                     /locus_tag="PK589_15310"
     CDS             complement(6621..7799)
                     /locus_tag="PK589_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNJ21029.1"
                     /translation="MNQTMQEQSTVKAHDHPEYSPGLEGVIAGESAICQVDEGEAGLR
                     YRGYAIGDLAGRSSFEEVAYLLLFGHLPATTELEEFSRLLVRNRRLPQQVHHFVESLT
                     PDMHPMDVLRTGISLLGLHDPDAQDGSREANLRKSVRLLAQIPHLVADSYQIMADRQP
                     ATPVSHGSFAENLLSLVAGRRYGEIGAAMAEALNVSLILYAEHEFNASTFAARVTAST
                     LTDLHGAITAAVATLKGPLHGGANEAVAAMLIEIGRPEHVEFWLRDQLAHKRRVMGFG
                     HRVLRQGDARSAIIQHHAERLSDLCGDRNWYHIATTLDRLMLEEKGLRPNLDFYTAVA
                     YLLMGIPRGLSTPLFVCSRITGWCAHVIEQQEHNRLMRPRALYTGPAPKAYEPLERRT
                     "
     gene            complement(7747..8685)
                     /gene="prpB"
                     /locus_tag="PK589_15315"
     CDS             complement(7747..8685)
                     /gene="prpB"
                     /locus_tag="PK589_15315"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HNJ21030.1"
                     /translation="MTFDHSSGDKGRRLQEIVARRTVAIPGASNALTAVQIERAGFDA
                     VYVSGAAVSAARGLPDIGLISLTEMVGEAGLIARAVAIPTIVDADTGYGPPSTVMNAV
                     REFERAGIAGMQIEDQEATKKCGHLEGKRLIPLGDMVAKISAAVKARHNRNFLIVART
                     DARTVDGLDGAIQRAKAYAEAGADMLFPEALLSPEEFGTFSLAIRNAGIHLPLIANMT
                     EFGRTPYLSVDEFQALGYQAVLFPVSSLRVAARAVEKLLSELKFFGSQRNWLDHMMTR
                     QELYDLIHYEDGTTSMRRSHEPDHAGTVNGEGSRPS"
     gene            complement(8682..10076)
                     /locus_tag="PK589_15320"
     CDS             complement(8682..10076)
                     /locus_tag="PK589_15320"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HNJ21031.1"
                     /translation="MLADRLAHYTQSLRFKDLPGDVVHEVKRRILDSLGCAFGAWTAT
                     PCRIAREMALAVKVPGGATVWGTNHKTLPDLATFANGAMVRYLDFNDTYLSKEPAHPS
                     DNIAAVLAAGETAHASGQLIIQALTLSYEIQCRLCDAAALRPRGWDHVTYGPLSSALG
                     IANVLKLTDAQTRQAVNLAGVANVALRQTRVGDLSMWKACAFSNAARNGMFAALLARR
                     GMTGPAPIFEGEKGFMKLVSGPFELPMLGGEAMPGGEPVPFKILKTCIKHFPVEYHAQ
                     SAVEAALALRAELLATEGKTWINKVADVEIGSYDVAIEIIGRDPEKWQPATRETADHS
                     FPYCVAVALLDGRVSMNSFSPKRLRDPRLHELMKKIHVVKLADLEHRYPTTMPTRLTI
                     RTSQGSTHSRQVDQPLGHPGHPLSDQEVGDKLRRLTSRRIDRAQLDRLLAFVWHLEDQ
                     TDIAAFMPLLRVRS"
     gene            complement(10108..10926)
                     /gene="meaB"
                     /locus_tag="PK589_15325"
     CDS             complement(10108..10926)
                     /gene="meaB"
                     /locus_tag="PK589_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNJ21032.1"
                     /translation="MNHVTAHPSFFRHDAAELARQVEAGEVRAVARVISLLENRDPLG
                     AAVLTHVAPSLNRALVIGITGYPGTGKSTVIDQLITAYRRLGEKVGVLAVDISSPVTG
                     GAVLGDRIRMQRHTDDARVYIRSMATRGHQGGLAAATRQAVRVLDAAGFGVILIETVG
                     VGQSDVDIMQVAHTVVAVVAPGLGDDVQAMKAGLLEVADIVVVNKGDREGADAALRDL
                     REWCKTVIRAVAVKGEGIAELIAVIAEHERWRDLDRPALHNRRGPSIDLGSRTG"
     gene            complement(10928..11875)
                     /locus_tag="PK589_15330"
     CDS             complement(10928..11875)
                     /locus_tag="PK589_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNJ21033.1"
                     /translation="MLCFATDKTRRLPVRIIEVSPRDGLQHEAIFVPTARKIDLINAL
                     SHSGVTEIEAGSFVSPTAIPQLADSDEVFRAIDRLPDVTYSALVPNERGLERARAAAV
                     QKIAVFTAASNSFTRHNIKATVQESLVRFTPVIHGAKRDGMLVRGYISTALWCPYEGR
                     IAPKLTVDVMRRLLDLGVDDISLGDTIGRASPADIRGLLDAVMPHIEPTRLSLHVHDT
                     YGMAVANVLTAWEDYAITAFDCSAGGLGGCPYAPGASGNVATEDVVFALKASGATVAV
                     DEELIVDCARQLSAVIGHPLHSRLSQISRPRIGQPALKV"
     gene            complement(11853..12698)
                     /locus_tag="PK589_15335"
     CDS             complement(11853..12698)
                     /locus_tag="PK589_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNJ21034.1"
                     /translation="MRPFTTLMIESNGAIARVTLNRPDRRNAFDGQMATELREAFEDL
                     AHDPSIRGIVLAAAGSVFCAGADLQWMQSDGPASETPAMRDAHHLLRMFRAIDEFPCP
                     VIARVQGSAFGGGLGLLAVCDIVVATEEATFALSEPRLGLIPAVIAPFLLHKAGESFL
                     RRYGLTGETFCASTAQRYNLAHDVVPQSNLDDRINELSDALMQLAPCATKESKLLFHR
                     LRALSDKERLALGPEYNARARCAPEAAEGLRAFIEKRPPAWAQPRERKPQEATTSDDV
                     VLRHR"
     gene            complement(12718..14322)
                     /locus_tag="PK589_15340"
     CDS             complement(12718..14322)
                     /locus_tag="PK589_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNJ21035.1"
                     /translation="MRVLDTSIRRDSDEFRRNQAHYEGLMADLQKHLEMVWAGGSREA
                     IALHKQRGKLTARERISSLLDPGSPWLELSPLAAFGLYDNQVPSAGLITGIGSVSGRF
                     CVIAANDATVKGGTYFPMTITKHLRAQTIALENRLPAIYLVDSGGVFLPMQAEVFADK
                     DHFGRIFFNQARMSAAGLPQIAVVMGMCTAGGAYVPAMCDENVIVQGTGTIYLAGPPL
                     VKAATGEEVTAEALGGADLHTRLSGVSDHLADDDRDALDICRSIIATLPRRLPKPRRT
                     HIDEPLYPAEDLYGLIPANPRQKWDVREVIARLVDGSRFHEFKARYGATLVCGFAQWM
                     GHMVGIVANNGVLLSESALKGAHFVQLCAQRRVPLVFLQNITGFMVGKDYESRGIIKD
                     GAKMVQAVSTAEVPKFTILIGASHGAGNYAMCGRAYGPRFLFSWPNARISVMGAQQAA
                     QVLVTVKEQQRAREKAALTDDERRRIIEATQRQYEQEGSPYFSSARLWDDGILDPTDT
                     RRVMGLCLDLAALAPVKDSHAPVFRM"
     gene            complement(14336..14743)
                     /locus_tag="PK589_15345"
     CDS             complement(14336..14743)
                     /locus_tag="PK589_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNJ21036.1"
                     /translation="MTVAATPLRILIGKVGLDGHDRGVKLVARALRDAGIEIIYTGLH
                     QSPEQIVTTAIQEDVQAIGLSIHSGAHNSLFPRILGLLRERGAEDIVVFGGGIIPDED
                     VPRLKAAGVQMLFRPATPMQDIVEFVNGLRTER"
     gene            complement(14740..15582)
                     /locus_tag="PK589_15350"
     CDS             complement(14740..15582)
                     /locus_tag="PK589_15350"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ21037.1"
                     /translation="MKLGAFDIYPVTDGRFRLDGGAMFGVVPKVLWQTCCPADDLNRI
                     PLSLTCLLIRAHGKHMLVDTGLGDKENEKFRSMFAVERKPTLRESLSQHGLCRDDIHL
                     VVNTHLHFDHAGGNTATDGRSAVAPTFPKATYYVQQTEYDDAMQANERTRASYRRDNF
                     TPVAEANQWALLKGETELLPGVTAIVTPGHTRGHQSVKVESEGRVAFFLGDLIPTVSH
                     LPLPYIMGYDLDPVQTLDSKRWVLERACEEHWLLLFEHDPYVQAGYVSKTVDGKYALQ
                     EVTL"
     gene            complement(15579..17174)
                     /locus_tag="PK589_15355"
     CDS             complement(15579..17174)
                     /locus_tag="PK589_15355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNJ21038.1"
                     /translation="MNERKSRFTSLSGLEIERCYGPDDLKGWSAEKDLGQPGAFPYTR
                     GCYPNMYRSRLWTMRQFAGFGSADDTNTRFKYLLDHGQTGLSVAFDLPTLMGLDSDDP
                     LARGEVGYCGVAISSLADMERLFDGIPLDQVTTSMTINGPAAVLFAMYLAVAEKRGIP
                     FERLGGTIQNDILKEYIAQKEWLFPPEPHLTLIVDTIAYCAAHVPKWHPISISGYHIR
                     EAGSTAIQELAFTLYNGLTYVEAAVKAGLEVDSFAPQLSFFFNVHNDFFEEIAKFRAA
                     RRLWAREMERRYHPKNPRSLQLRCHAQTAGCSLTAQQPMNNVVRTTLQALAAVLGGTQ
                     SLHTNSMDETLALPTEEAVKLALRTQQIIAAESETVNSVDPLGGSYVVESLTNRLEEG
                     ALDLFRKLDGLGGMVSAIERGFPQREILDASQRYQREIERQERSIVGVTTHREEDVRS
                     IPILKIGPEVEQEQVARLSDLRKSRDPFKMAGSLEELQEMAACHQNLMPSLIEAVKAK
                     ATLGEICAALKEVYGTYREPVVL"
     gene            complement(17206..>17535)
                     /locus_tag="PK589_15360"
     CDS             complement(17206..>17535)
                     /locus_tag="PK589_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ21039.1"
                     /translation="DFPGFIVNRVLIPMINEAIFALEDGVATAEAIDLAMVEGTNHPV
                     GPLALADRIGLDTVLAICEVLHQDLGDPKFRPCPLLRKYVEAGWLGRKSGHGFYQYGD
                     QPVMHLT"
BASE COUNT         3450 a         5256 c         5264 g         3565 t
ORIGIN      
        1 attccaagga gggaggaagg gaaggtgaac tgggcggagc gatcagcgtg aaaatcgata
       61 tgagcaagct cgcggttcgg taaatttata ggaagacgct cgcgaagggt ccataggccg
      121 agaatcgctt ttctagctga ttgagctgtg cggtctgctt ctaaagtaag ctccacggtt
      181 tgggagctgt cgaacatgag ttccgtctcg tccatccggc agccgagtcc ttagttattt
      241 atcaattcag tgcgatctgc cagtatcgtc atcccacgag cgctgaactc gatgatgctc
      301 tgtcgtctcc acaccgtgcc gagtggtcac tcactctacc gcgcaatacc ggaaggcgga
      361 agtagaatag ccgtatagat ggggcggaca aaggtgtatc agtagcacca agtcttcccc
      421 agttcagagt gtctatactt ctccacccgg ttacgctgcc gataatgcct cttcgctgca
      481 aacaaagtcc gcaccagttc tgctcgatgg gaaatgcccc gtttccagtt gacgagggaa
      541 ggggtgatcg tacaaaatat tgtacaattg taaagaggtg gaattcatgg tcaagacgct
      601 ccctatctct gaagctcgcg agcagctccg tgccctcgtg gaacaagcaa atcggacgat
      661 ggcccgcgtg attattacgc ggaatggcaa acccgaggcg gtcttgatgg gctatgccga
      721 atatgaaagc tggctagaga cgcttgacgt catggccgac catgacgaat tggcagcgat
      781 tgaggctggc ctggcggacg tgcgggccgg gcggttggtc tcagccgagg atgcgtatcg
      841 cgaggctgca ccccctaaag atacccctct tgaaggccca ggaaacatag ggagtggcgg
      901 gctgaccgag cgcgaagtga cggtgctgca tcgcattgcc gccggatcac tagagaatga
      961 gattgcccac gaccttcgcc tcgcgccgaa gaccatccat gcacttaaaa tgagcatttt
     1021 tcgtaagctg gggattcgga ctcatctaga agcacggcat gacaccgttc aaaaacgtaa
     1081 agctgacaaa gtcggccgct aaggaactcc gcccgatccc gcagaaattc cgagcagctg
     1141 tccaggcggc gcttgatgcg ttggcagccg accccctcct gggcacggct ctccttccgc
     1201 cgtttgaagg ttgccgccgc tatcgcatcg ggaactatcg catcgtctac cagtttgatg
     1261 cgacgacgct cattgttctt agtattcgcc atcgcaaaga cgtgtaccgc tgacgcctca
     1321 caaaatcacc gcaactacta gctctgtgta ttcaccacag tttgccgtgg tccgaagatt
     1381 catctctggt gatctgctct tcctaagaga ggtaaagatc accgtcactc gtttagccaa
     1441 tcttgaaaag tagacgttag atactgcctc tgattatttc cctgagggaa atcgcgaatg
     1501 gggcctgaga ctggatagtc ggaatcgaat gggggaacag agacggcaaa tccgcaaccc
     1561 aggcgcaacc tgaactgatt gcagccgtag cagatcacgc taactggctg gtttattggt
     1621 ggcggtgacc cggattgaac gggtgacccg cggcttatga gtccgctgct ctaccaactg
     1681 agctacaccg ccacagcacg atcggtcgtg tgaagaagag aacgcggata ataactgaaa
     1741 tgcccccgaa gtgtctaccg attgcctgac gaaaacctgg aaggctagga attgggcctt
     1801 ggacagaatc gtcgggggat ggtatgacgc taagccgaat gttccggctc gtcttcttgc
     1861 catttgcagc gtcgcgcggc attcggccga aactctccgt ctccctgtga tcgcagaagc
     1921 gcgccggtcg cgctcataaa ttggaggcct gcatgatgac cggaatctca ttggacacga
     1981 tcctggcctg gttgatcacg atgtccgcgg ccgtggggat cgccagtttg cgcatcgggt
     2041 tggtcctggc gatcgggtat gtggcaatcc ggttcgtgcg ccttggcctg catcagctcg
     2101 aacgagtgat gattgtggcc agcgataagc aggaccagga atccggaacc gccgccaagc
     2161 gtgcagccac gttgacggga attctccgga ccatcgccct gtcggcaatt tgggcgatca
     2221 tcatcattga atcgctgcag ctggttggat tggatatcgc cccgatcttg gccggcgccg
     2281 gcattctggg cctcgccgtc ggcttcggcg cgcagaatct ggtgcgcgat ctgatcagcg
     2341 gcttcttcat cattctggag gatcaggtcc ggctgggaga tgtggctgtg atcaacggga
     2401 ccggcggggc ggtggaaacg attaccttcc gcaccatctc gctacgcgac ttctccggcg
     2461 tggtgcacat tttccccaac ggcggcatca cgacgttgtc caatatgacg aaagactggt
     2521 cggcctttgt tctggatatg ggcgtggcct atcgggagga cacggaccgg gtcgtcgaag
     2581 tgatgcggac agtgggagaa gggttgcgcc aggatcaggc gttcggttcg ctgatcatcg
     2641 agccgattga aatcgtgggc gtcgaaaatt ttgccgatag cgccgtgacc attcgcgcac
     2701 gaatcaaaac gaaaccggct gaacaatgga aaatcgggcg cgaataccga cgccgtctca
     2761 agaaggcctt cgatgctcag gggatcgaca ttcccttccc catgcgcacc ctgttgatgg
     2821 gagaaaccga ccagcccttg aaggtcgagg tgatcgccgg cctggggctt ggaaaaagtt
     2881 agctcagtga gcgatcacgt ggatctctcg cctgccttcg ccttgacagg cgcgaagaga
     2941 gaccggtcct tcacgtctag ggacacggat gccatggcca gtggcatcgc ttctgatacg
     3001 gcctgcttgc gacggtaatc atctaaacgt cgatgcacga tgccgtccga caggccagga
     3061 taatatccct tcagcgcttg ttgtccggtc tccgcggtga cgccgtcgca atggcgccgg
     3121 atttcacgaa ggcgttgcgt aatccgtttc cgtgccgcga catgaaaggc ctctgcgagc
     3181 tcccataccg tcgtgcgtcc ctccaatctg gtttgctgct cggcatacag caccgtggcg
     3241 atcatggcca acaggtcttc tccgatggat tcgatccggt tctgcacccg ctgctcatct
     3301 tgaaacgcag cctggaagcg caccatcgca taaaaaattt cccgcgccag gcgccggctg
     3361 gttcgttcga cataccgggc aacggaccgg acttgcggat gcgcaaaggc cgagcccgtc
     3421 ggcagggagt gccgtaccca gtgccgcagg taccagggcc cataaaatcc ccccagtttc
     3481 acgagctgct tgagttggtc gagaatcgac aggtcgccgg aatagaacgg cttcgcgcgg
     3541 tcgagatggt gactcagccc ttcacgcacg ataaaggggc gcaggatgtc cgtggctccc
     3601 tcaccgatgc gatacatctc cgcgtcccgc acgagctgtt cgatcccgaa tcccgcttcg
     3661 ccgcgcgcca ttttggaggc cgcggtttca taccccatgc cgccgaacag aatttgtgcg
     3721 tcccggagaa attgaatcgt atgctcggag cagaaaatct tcgcgacggc cgcctcgata
     3781 cggatgtcat gacgatgctg atccgccagt cgccacacca gcatggagag cgcttccatg
     3841 gcaaacaaat gcgcagccat gtcgcccagg cgaatctgct gggtttgacg ttctgccagc
     3901 ggcttgtgaa acgtcacccg accgttggcc cgatccaaag tcggctgcca ggcctgcttc
     3961 gccatgccca gacaaatggc cgggatgctg atgccgcgcc ccacgtttaa aatcgtcagg
     4021 gcatatttca accctttccc tacctcgccg atcacgttct cgaccggaat ccggacctcc
     4081 tgcatcgtca tgtgggcgtt ttcaattccc cggcaccctt cgaactggca gcgttgacgg
     4141 acctgcatgc ccggcgtgga catatccagg ataaacgccg tatgcacccg gtcgtcggct
     4201 ccctgtcctt caggcaccgg catccaggtc gtctgtccct ctcgaaccac tcgtcgagcc
     4261 gggacacggg cgatgagcgt ggcataacgc gcgatcggtc cattggtgca ccagagtttt
     4321 tcgccgttca cgaggaagtg gctgccattc gcatcgagcg tcgcttccgt ccggacattc
     4381 gcggcatcgg acccggtcat tggttccgtg agcgcgaagg cggagagcgc ttctgtcgcc
     4441 accagcggaa gatacctccg cttctggtct tcgttgccga acaagaggat cggcatggcg
     4501 atgccgatgg actgcggcac ggccacggtc aaggcgagaa tgttgctcca actcgcaatc
     4561 aacgtaagga cgcggccata gtttgtatag gagaagccca gcccgccata ggccttcgga
     4621 attttcatcc cgaaggcgcc tagtttgaac aagccccgaa tcaccgcgtc gggaattttc
     4681 gcctcacgct cgatttcgtc cggatccacc tcctgccgta ggaatgtttc gacctgttta
     4741 cagaaggcca tgccggccgc ccgttcctcc ggtggttcgg ggggcgtcag caggaactcg
     4801 aagtccggcc ggccgtcaaa gagtccggcg agaaaacttg ccccggtcaa tcgctcccgc
     4861 gcttcctcga tccgttcgaa tcctttgatg agggtgctca tgaatgacct cacacggcct
     4921 gttcaactca ctgatctctg aactgtgtgt cgcgatactg cgccagcctt cctgccaacc
     4981 tcgctttcaa ctccagccgc ttgggtttgc ctgtcgaggt gtagggcaca tcatcaccga
     5041 acagtacgac cttaggttgc ttggaaaaag gcaaccgcat ccggcaatgc gccagcacat
     5101 cagcttcgct cgggatggag gcgggatctt tgggcaccac gtaggccgcg atttcctcgc
     5161 cgtagtagcg gttctcgaac ggcagggcca tcgcaaactt cacggcctgg tgcgtcttca
     5221 gcacgtcatc gatttccagc ggagaaatgt tcacccctcc tcgaatgatg agttccttca
     5281 atcgtcccga cacgaagaag aacggccgtc cgctgtcatc gaggagatag aatccctcat
     5341 ctcctgaccg gaaccatccc cattggaagg cggcttcgtt ggcgtcatcg cgcttaaaat
     5401 agccggcaca gacggtgcgt ccgcgaatac aaatttctcc gcgggtggcc gggggcagcg
     5461 tgcgtccacg ttcgtcgagg accgtcatga ggttgtgacg aaccggcact cccacggagg
     5521 gaagctcata gccggtgagc cagtgacggt gctgttcctg cgtcagatcg ttcggcagga
     5581 aacaggagta acaggtggtt tcggacaggc cataaccatg gcgaatgacg acgccgaagc
     5641 gatgttcgaa acgcgcggcc gtatctttca agagaggccc ggcgccgcag atcaacccgc
     5701 cgaggcggct cagatcgtag ggggtgagat cctcatcggc atcgagcaga aattcgagca
     5761 gcgtggggac gacactgacc gaggtgacct gctcttcatg aatccgtcgc cagaagctgg
     5821 tggtcttgaa tctgcggttc atcaccacac tgcccttgca gtacaacggc gtgatcagcg
     5881 tgacgacgat accgttcaca tgatggatgg gcaggacgca catcaggcga tcatggagac
     5941 cgaagctgtg ccagctcgcg atcgcatccg catcgatcag aagattggcc accgtcagga
     6001 tgacgccttt cggtgggccg gtcgtgccgg aggtgtagac gatcagcgca tgatcgtcga
     6061 gccctgacgt gaacggagag cggcgaagcg agcggcctgc tccgtgtgcg tgaaattttt
     6121 ttccggtcct gtgattatcg agcacaccgg catcacccag cgcaatgacc tggcgcaacg
     6181 tggggatgtc cgactggagt gcgcagagtt cttcatagcc gtcctgccag cagaagaccg
     6241 ttgagacctc tgattgttcg agaatgtatc gctttttctc cggcgtttct tcgatgttga
     6301 tcgggacgac tgcgactccc agcgtccagg ctgcgaggta ggtgagcacc gtcacatcgt
     6361 ggttgaagag caacgtggcg acccggtctc cacgggtcag gccgaatctg gtatggaaga
     6421 ctccggccag ccgttcgatg atcgctccca gttcggcata ggtgtaggtt cggcgcagag
     6481 cacgatcgtc gtcacagaac gtcagtgcat gcgttgtggc gagctgcggg tcgtagacgc
     6541 gggaacgaag gaattccgcg aacgacgccc aaggcacatc cgttgcgcgt ccagatacgt
     6601 cgcgagcctg ctcgatatgg tcacgtacgt cgttcaagag gttcataggc tttcggcgcg
     6661 ggccccgtgt agagcgcccg tggtctcatc aaccggttgt gctcctgctg ctcgatgaca
     6721 tgtgcgcacc agccagtgat ccgtgaacag acaaacagcg gcgtggatag tccgcgaggg
     6781 atgcccatga ggagataggc cacggctgta taaaaatcga gattcgggcg cagtcccttt
     6841 tcctccaaca tgagtcgatc gagggtggtg gcgatgtggt accagttgcg atcgccgcag
     6901 agatcgctca atcgctcggc gtggtgttgg atgatggccg accgcgcatc gccttgccgg
     6961 agcacgcggt ggccgaatcc catcacgcgg cgcttgtgcg ccagctggtc tcgcagccag
     7021 aactcgacat gctccggccg gccgatctcg atcaacatcg cggccaccgc ttcattggcg
     7081 cctccatgga gtggaccttt cagcgtagcc actgccgccg tgatggcgcc atgcaggtcg
     7141 gtgagtgtgg acgcggtgac ccgggcggca aaggtggagg cattgaactc atgttccgca
     7201 tacagaatca gcgagacgtt cagcgcctct gccatggcgg ccccgatttc cccatatcgg
     7261 cgccctgcaa ccagactcag aaggttttcg gcaaagcttc cgtgagaaac cggcgtggcg
     7321 ggttgccggt ccgccatgat ctggtagctg tcggcgacca ggtgagggat ctgcgcgagc
     7381 aatcgcaccg atttgcgaag gttcgcctcg cgggatccgt cttgcgcatc aggatcatgc
     7441 agcccgagca gtgaaatccc ggttcgcaac acatccatcg gatgcatgtc gggcgtgaga
     7501 ctctccacga agtggtgcac ctgctgagga agccgacgat tgcgaaccag gaggcgagag
     7561 aattcctcca attccgttgt ggcagggagg tggccgaaga gcagcaggta ggcgacctcc
     7621 tcgaagctgc ttcgtccggc gaggtcgccg atggcatacc cgcgataccg cagccccgct
     7681 tctccttcat cgacctgaca gatcgccgat tccccggcaa tcacgccctc caggcccggg
     7741 ctgtattcag gatggtcgtg agccttcacc gttgactgtt cctgcatggt ctggttcatg
     7801 tgacctccgc atggatgtgg tgccatcctc gtagtgaatc aggtcgtaca gttcctgcct
     7861 ggtcatcatg tggtcgagcc aattccgctg ggagccaaag aacttcagct ccgataggag
     7921 tttttcgacc gcgcgcgccg cgacccgtaa cgagctgacg ggaaacagca ctgcctgata
     7981 gcccagggct tggaactcgt ccacgctgag atagggcgtt cggccgaact ccgtcatgtt
     8041 cgcaatcagc gggagatgaa tgcccgcgtt tcgtatcgcc agcgaaaagg ttccaaactc
     8101 ctccggtgat agcagcgctt ccgggaacaa catatctgcg ccggcctcgg cataggcctt
     8161 ggcccgttgg atcgcgccat cgagtccatc gacggtccgc gcatcggtgc gcgccacgat
     8221 cagaaagttc cgattgtgcc tggccttcac agcggcgcta attttggcga ccatgtcgcc
     8281 gaggggaatg aggcgtttgc cctcgagatg gccgcatttt tttgtcgcct cctgatcttc
     8341 gatctgcatg ccggcgatgc cggcgcgctc gaattcacgg actgcattca tcacggtcga
     8401 aggaggccca tacccggtgt cggcatcgac aatggttgga atggctacgg ctctggcgat
     8461 gagtcccgct tcgccaacca tttccgtcaa ggaaatcagg ccgatgtccg gcaggccgcg
     8521 cgcagcagac acggcggcgc ctgatacgta gaccgcgtca aatcccgctc gttcaatctg
     8581 aacggcggtc aatgcattgg acgctcccgg gatggccact gtgcgcctgg ccactatctc
     8641 ttgcaatcgt cggcctttgt cgccgctcga gtgatcgaac gtcatgatcg caccctcaac
     8701 agcggcatga atgcggcgat gtctgtctgg tcctcgaggt gccagacgaa ggcgagcaag
     8761 cgatccagtt gcgcgcgatc aatgcgcctg gacgtgagcc gccggagttt gtcccccact
     8821 tcctgatccg acagaggatg gcctggatgc ccgagtggtt gatccacctg acggctatgc
     8881 gtcgagcctt gtgacgttct gatcgtgagg cgagtcggca tggtagtcgg gtagcgatgt
     8941 tcgagatccg ccagtttcac gacgtggatt ttcttcatca gctcgtggag acgcgggtct
     9001 cgcagccgtt tcgggctgaa tgagttcata cttacgcggc cgtcgaggag agcgaccgcc
     9061 acgcagtacg gaaagctgtg gtcggccgtt tcccgcgtcg cgggctgcca cttttccgga
     9121 tcgcgtccga taatttcgat ggcgacgtcg tagctgccga tctcgacatc ggctacctta
     9181 ttgatccagg tctttccttc tgtcgccagc agctccgctc gcaacgccaa tgcggcttcg
     9241 acggcgcttt gtgcgtggta ttccacgggg aagtgtttga tgcaggtctt cagaattttg
     9301 aagggcaccg gctctccgcc cggcatcgct tccccgccca acatcggcag ttcgaatggg
     9361 ccggagacca gcttcatgaa ccctttctcg ccttcgaaaa tgggggcggg gccggtcatg
     9421 ccgcgccggg cgagcagggc ggcgaacatg ccgttgcgtg cggcgttgga gaatgcgcag
     9481 gctttccaca tcgacagatc gccgacccgt gtctggcgca gagcgacatt ggccacgccg
     9541 gccaggttga cggcctggcg ggtctgcgcg tccgtcagct tgaggacgtt ggcaatgccg
     9601 agtgcggacg agagggggcc ataagtgaca tggtcccagc ctcgtggccg caaggccgct
     9661 gcgtcgcaga gccgacattg gatttcatag gacagggtga gcgcctgaat gatgagctgg
     9721 ccggaggcat gcgctgtttc ccccgcagct aggactgccg cgatattgtc tgagggatgc
     9781 gccggttctt tggacagata ggtatcgttg aagtcgaggt agcggaccat ggcgccattg
     9841 gcgaaggtcg cgaggtcggg aagggtcttg tgattcgttc cccacaccgt cgccccgccg
     9901 gggaccttca ctgcgagcgc catctcccgc gcgatccggc agggggtggc agtccatgca
     9961 ccgaatgcgc agcccagact gtccagaatg cggcgcttga cctcatggac cacgtcgccg
    10021 ggtagatcct tgaaccggag cgattgcgta tagtgagcca gtcgatcggc cagcatgcac
    10081 cacctcatgg caatgacagc agcagcgtta tcccgttcgc gagccgaggt cgatggaggg
    10141 ccctctccga ttatggagtg ccggcctgtc cagatctcgc caccgttcgt gttcggcgat
    10201 gaccgcaatc agttccgcga tcccttcccc cttcaccgcc acggcgcgaa tcaccgtttt
    10261 gcaccattca cgtaaatccc gcagggctgc atccgcccct tcgcgatcgc ccttgttcac
    10321 cacgacgatg tccgcgacct ccaacagtcc cgccttcatg gcctgaacat cgtcgccgag
    10381 ccccggcgcg actactgcga ccaccgtatg ggcgacctgc atgatgtcga cgtcgctttg
    10441 gccgacgccc accgtctcga tcagaatcac gccaaagccg gcggcgtcca agacccgtac
    10501 tgcctgccgc gtggcggcgg cgagcccgcc ctggtgcccg cgtgtcgcca tgctgcggat
    10561 gtagacccgg gcatcatccg tatggcgctg cattctgatg cgatcgccga ggaccgcgcc
    10621 gcctgtgacg gggctgctga tatccaccgc cagcacgccg accttctctc ccagtcggcg
    10681 gtaggcggtg atgagctgat cgatcaccgt actttttccg gtgccgggat acccggtgat
    10741 cccaatcacc aaggcgcgat tcagagacgg agccacatga gtcagcaccg ccgctccgag
    10801 cggatcccgg ttttccagca aactgatgac acgcgccacg gctcgaacct ccccggcttc
    10861 gacctgtctc gccagctcgg cggcatcgtg tcggaagaag gatggatgcg cagtgacgtg
    10921 gttcacatca caccttcagg gccggttgcc cgatacgggg ccgcgatatc tgtgacagcc
    10981 gcgagtggag gggatgtccg atcactgcgc tcaactgacg cgcacagtcg acgatcagtt
    11041 cttcgtcgac cgcgaccgtc gccccggagg ccttcagggc aaacaccaca tcttccgtcg
    11101 cgacgttgcc ggacgcaccg ggcgcatagg gacaaccgcc cagaccgccg gctgaacaat
    11161 cgaacgccgt gatcgcatag tcttcccagg ccgtcagcac attggccacg gccattccgt
    11221 aggtgtcatg gacatggagc gacaggcgag tcggttcgat atgcggcatg acggcatcca
    11281 gcaagccgcg gatatctgcc ggcgacgctc tgccgatagt gtctcccaga gagatgtcat
    11341 ccacaccgag atcgagcaac cgccgcatga cgtccacagt cagtttgggc gctatgcgcc
    11401 cctcataggg gcaccacagg gcggtggaga tatagcctcg caccaacatc ccgtcccgtt
    11461 tcgctccatg gatcacgggg gtaaagcgaa ccagggattc ctggacagtt gccttgatgt
    11521 tatggcgcgt gaaactgttc gaggcggccg tgaagacggc gatcttctgc actgcggccg
    11581 cccgtgcccg ttccaggccc cgttcgttcg gcaccagggc ggaataggtc acatccggaa
    11641 gccggtcgat agcgcggaac acttcatcgg agtcggccag ttgcggaatg gcggtcggcg
    11701 aaacaaagga accggcttcg atttctgtca caccgctgtg tgacagggca ttgatcaggt
    11761 cgatcttccg cgccgtcggc acgaagatcg cttcgtgttg cagtccgtca cgaggtgaga
    11821 cctcaatgat ccggacaggc aaccggcgtg tcttatctgt ggcgaagcac aacatcgtcc
    11881 gacgtcgtcg cctcctgcgg cttccgctca cgtggctggg cccacgcagg cggccgtttt
    11941 tcgataaagg cgcgcagccc ttcggctgct tccggagcac aacgggcgcg ggcattgtat
    12001 tccggcccca gggccaggcg ttctttatcg gatagggccc gcagccggtg aaacaacagt
    12061 ttactttcct tggtggcaca gggcgccagc tgcatgagcg cgtcgctcaa ttcattgata
    12121 cggtcatcca gattgctctg tgggacgacg tcatgggcca ggttgtatcg ctgggccgtc
    12181 gatgcgcaga acgtttcgcc ggtgaggccg taccgtcgga gaaacgattc gcccgctttg
    12241 tgtaagagaa acggcgcgat gacggccgga atcaacccaa gccggggttc gctcaaggca
    12301 aaggtggcct cctcggtcgc cacgacgatg tcgcagacgg ccaagagacc aagtccgcct
    12361 ccgaatgccg atccctgcac ccgggcgatg accggacaag gaaactcatc gatggcgcga
    12421 aacatgcgca gcaggtgatg ggcgtctcgc atcgccggag tttccgacgc cggcccgtcg
    12481 gactgcatcc attgaaggtc ggctcctgca caaaagacgg agccggcagc ggccagcacg
    12541 atccctctga tcgacggatc gtgcgccagg tcttcaaagg cttcccgaag ttccgtcgcc
    12601 atctggccgt cgaaggcatt ccgacggtcg ggtcgattga gcgtcacacg cgcaatggcg
    12661 ccattcgatt caatcatgag cgtggtgaat ggccgcattc gcacctcctc tggctgctca
    12721 catacgaaac accggcgcat ggctgtcctt cacgggcgcc aatgcggcga gatccaggca
    12781 gagtcccatc acgcggcgcg tgtcggttgg atccaaaatg ccgtcatccc acaagcgggc
    12841 gctgctgaag taagggctgc cttcctgttc atattgccgc tgggtggcct cgatgatgcg
    12901 gcgtcgttcg tcatccgtta aagcggcctt ctctcgcgct cgttgttgtt ccttcaccgt
    12961 gacgagcact tgggctgcct gttgcgcgcc catcaccgag atgcgcgcgt tgggccacga
    13021 gaacagaaat ctcgggccgt acgctcgccc gcacatggca tagttgccgg ccccatggga
    13081 ggcgccgatc aggatcgtga acttgggcac ctccgcagtc gacaccgcct ggaccatctt
    13141 ggccccgtct ttgatgatgc cccggctctc atagtccttt ccgaccatga agccggtgat
    13201 gttttgcaag aacacgagcg gcacgcgacg ctgggcgcaa agctggacga aatgcgcgcc
    13261 tttcaacgcc gattcggaca gcagcacgcc gttgttggca acgatcccca ccatatggcc
    13321 catccactgc gcaaagccgc agacaagcgt agcgccatat cgcgccttga attcgtggaa
    13381 gcggctgccg tccacgagcc gggcaatcac ctcacggacg tcccactttt gccgcgggtt
    13441 ggcggggatg agtccataga gatcctctgc tggatacagt ggctcgtcga tgtgggtccg
    13501 ccgcggcttg gggagtcgcc gaggcaatgt ggcaatgatg gaacggcaga tgtccaacgc
    13561 gtcacgatcg tcatcggcca aatggtcgct cacaccggag agtcgtgtgt gcagatcagc
    13621 cccgcccaat gcctcggcgg tgacctcctc gccggttgcc gccttgacca gtggtgggcc
    13681 ggccaaataa atggtgccgg tcccctggac gatgacattc tcatcacaca tcgccggaac
    13741 gtaagcgccg ccggccgtgc acatgcccat gaccacggcg atttgaggaa gaccggccgc
    13801 ggacatgcgc gcctgattga agaagatccg gccgaaatgg tccttgtcgg caaagacttc
    13861 agcctgcatc gggaggaaga cgccgccgga atccaccagg tagatggccg gcagccggtt
    13921 ctccaacgcg atggtttgcg cgcgaagatg tttggtgatc gtcatcggga agtaggtgcc
    13981 gcctttgacg gtcgcatcgt tcgctgcgat gacacagaac cggccggaca ccgaaccgat
    14041 gccggtaatg agtccggctg acggcacctg attgtcatag aggccgaacg cggccagggg
    14101 actcagttcc agccagggag aacccggatc gaggagtgat gaaatccgtt cgcgggcggt
    14161 gagtttgccg cgctgcttgt ggagcgcgat cgcctccctg gatccgccgg cccacaccat
    14221 ctccagatgc ttctgcagat cggccatcaa gccttcgtaa tgggcctggt tccggcgaaa
    14281 ctcgtccgag tctcgtcgta tggacgtgtc caacacacgc atggctcgaa tcctctcagc
    14341 gttcggttcg aagcccgttc acaaattcca cgatgtcctg catcggtgtg gcgggcctga
    14401 acaacatctg cacgccagcc gccttcaatc gcggcacatc ctcgtcgggt atgatgcctc
    14461 caccgaagac cacgatgtct tccgcgcctc gttcccgcag cagtccgagt atgcggggga
    14521 acaaggaatt atgtgcgccg gaatggatgc tgagaccgat ggcctgcaca tcttcctgaa
    14581 ttgcggtcgt cacgatctgt tcagggctct ggtgaagccc tgtgtaaatg atctcgatgc
    14641 ccgcatcacg aagcgcgcgg gcgacgagct ttacaccccg gtcatggccg tccaatccca
    14701 ccttgccgat cagaatgcgc aagggtgttg ccgcgacggt catagcgtca cctcctggag
    14761 agcatatttc ccgtccacgg ttttgctgac gtatccggcc tgcacgtacg gatcgtgctc
    14821 gaagagcaac aaccaatgtt cctcgcaggc acgctccagc acccatcgtt tgctgtcgag
    14881 cgtctgaaca gggtcgaggt cgtaccccat gatgtaggga agcggaagat gggacacggt
    14941 gggaatgaga tcgccgagga aaaacgcgac ccggccttcg gattccactt tgacactttg
    15001 atggccgcgc gtatgcccgg gtgtgacaat ggccgtcact cctggcagga gttccgtctc
    15061 acccttgagc aaagcccact ggttggcctc cgccaccggc gtgaaattgt cccggcgata
    15121 actggccctg gtccgttcgt tggcctgcat cgcatcgtcg tattctgtct gctggacata
    15181 ataggtggcc ttgggaaacg tcggcgccac tgcgctgcgt ccatcggttg ccgtattgcc
    15241 tcccgcgtgg tcgaagtgca gatgcgtatt caccaccaga tggatatcat cgcggcagag
    15301 gccatgttgc gagagggact ctcggagagt gggctttcgt tcgaccgcaa acatcgagcg
    15361 gaatttctca ttctccttat cgccgagccc cgtatccacc agcatatgct tgccgtgggc
    15421 ccggatcagc aggcaggtca ggctgagcgg aatgcggttc aggtcatccg ccgggcaaca
    15481 ggtttgccac agcactttcg ggacaacccc gaacatggcg ccgccgtcca gccggaatcg
    15541 gccgtcggtt accgggtaga tgtcaaaggc gccgagtttc ataagaccac cggttcccga
    15601 taggttccat acacttcctt caaggcggca caaatctcac ccaacgtcgc cttcgccttc
    15661 acggcttcga tcaatgaggg catgaggttc tggtgacagg cggccatttc ctgcagctct
    15721 tccagcgagc cggccatctt gaaggggtcg cgtgattttc tgagatcgga cagtcgggcc
    15781 acctgttcct gttcgacttc cgggccgatc ttcaggatgg gaatggatcg aacgtcttct
    15841 tctctatgcg tcgtcacccc gacgatgctc cgttcctgcc gctcgatctc acgctgatat
    15901 cgctgcgagg catcgagaat ctcccgctgt ggaaacccgc gctcgatggc ggagaccatg
    15961 ccgcccagtc cgtccagttt gcgaaagagg tccagcgctc cttcctccag gcggttcgtt
    16021 agcgactcga caacatatga tccgcccaat ggatcgaccg aattgaccgt ctcgctctcg
    16081 gccgcaatga tctgttgcgt gcgtaacgcc agtttcacgg cttcttccgt cggcagcgcc
    16141 agcgtttcat ccatggaatt cgtgtgaagt gattgtgtgc cgccgagcac ggcggcgagg
    16201 gcctgcagcg tggtccgcac cacgttgttc atcggctgtt gggcggtgag ggagcaccct
    16261 gcggtttggg catgacagcg gagttggagt gaacgggggt tctttggatg gtaccgccgt
    16321 tccatctcgc gggcccagag gcgccgggct gcgcggaatt tggcaatctc ttcgaagaaa
    16381 tcattatgga cgttgaagaa aaaggagagc tgcggtgcaa acgaatccac ctcgagaccg
    16441 gccttcacgg ccgcctcgac gtacgtcagc ccgttataga gcgtgaaggc gagttcctgg
    16501 atcgccgtcg agcctgcttc ccggatgtga tacccgctga tgctgattgg atgccacttc
    16561 gggacatgag ccgcacaata cgcgatggtg tcgacgatca aggtcagatg cggttccggc
    16621 ggaaacagcc attctttctg ggcgatgtac tccttcagaa tgtcgttctg aatcgtgccg
    16681 cccagtcgct cgaacgggat cccgcgtttt tctgccacgg ccagatacat ggcgaagagg
    16741 acggcggcgg gtccgttgat cgtcatggag gtagtgacct ggtcgagcgg aatgccgtcg
    16801 aacagtcgct ccatgtcagc gagggaggaa atggcgaccc cgcaatatcc gacttcgccg
    16861 cgggcgagcg gatcatcaga gtcgagaccc atgagggtcg gcaggtcgaa ggccacgctc
    16921 aggccggttt ggccgtgatc cagcagatac ttgaagcgtg tgttggtgtc gtccgctgag
    16981 ccgaacccgg caaattgccg catggtccag agccggctgc gatacatgtt gggataacag
    17041 ccgcgggtgt aagggaaggc cccaggttgc ccgagatcct tctcggcgct ccagcccttg
    17101 agatcatcgg gaccgtaaca ccgctcgatc tccaagccgg acagggacgt gaatcgggat
    17161 ttccgctcgt tcatgacgcg ttcctccaat cacggacagg aaacgtcagg tcaggtgcat
    17221 taccggctga tctccgtact gataaaatcc atgtccgctt ttcctgccaa gccagccggc
    17281 ttctacgtat ttccgcaaca gcgggcaggg gcggaatttc ggatcaccca agtcctgatg
    17341 gagcacctcg cagatggcga gtacagtatc caggccgatc cgatctgcca gtgccaaagg
    17401 accgacagga tggttggtcc cttccaccat cgccagatcg atggcttccg ccgtcgcgac
    17461 cccatcctcc agcgcaaaaa tggcttcgtt gatcatggga atgagcaccc gattgacgat
    17521 gaagccggga aaatc
//
LOCUS       DAOQDS010000075        17427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1319_length_17427_cov_17.720124, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000075 DAOQDS010000000
VERSION     DAOQDS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17427
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1319_length_17427_cov_17.720124"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PK589_15365"
     CDS             <1..654
                     /locus_tag="PK589_15365"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNJ21040.1"
                     /translation="IANSPAYMTRNPVVSLQHAVSMLGMNLMSELAFSASIKGSAFKV
                     PGWDGEVKSLWHYSLASGAYAKEIARMRRFNVESAYLCGLLHGIGRPVVLQTLVALAK
                     EQGSPLTKELLHQLLDGYYIQVGLLVADEWGLPPPVVESIGFHADYHYAKTAKQECMT
                     TCLAGRLAKHFLDPEGFDEATVRDHSVFADLNLYPKDIDALLAHKDKVQAVVEAMPL"
     gene            654..2072
                     /gene="sthA"
                     /locus_tag="PK589_15370"
     CDS             654..2072
                     /gene="sthA"
                     /locus_tag="PK589_15370"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNJ21041.1"
                     /translation="MPATAYDIVVVGSGPAGQKAAIQGAKAGKKVVLIEQEPGIGGNC
                     VYRGTIPSKTLRETAVQFERLKRSSEVFEGKMRIDVPMSVLLHRLDEVVKAHECYMAS
                     QLTRNGVTYRHGRARLLSPHEVQLETVDGACQTLTAETIVLATGSRPSSIPEIPVDHE
                     HVLDSDSILSMIYLPRSLTILGGGVIACEYASTFALLGVEVTIIDRAKRPLPFMDHDI
                     VDVFQRSIERQGGRFYSGHTVTEVAWDGVSSVVARLANGMAVKSEKMLVALGRQPNVE
                     ELNLEAAGLSLDEKGHIPVNDHGQTAVPHIFAAGDMLGRPPALASQAMEDGRRAVSHA
                     LGLPVGDSLNQVPIGIYTIPEIASIGLDEEQAAARYRGPIIGRAPFTEIAKGQITGAC
                     DGLLKLVSDPSGERLLGVQIVGENATELIHLGQMALQDGATIDRFIDTIFSFPTFAEA
                     YRVAALDILGQRRKRRDEAQAA"
     gene            2167..>2687
                     /locus_tag="PK589_15375"
     CDS             2167..>2687
                     /locus_tag="PK589_15375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ21042.1"
                     /translation="MNREERRREEKLQGRNAGGPAAALAGLREAQMHHQAGRLDDAER
                     GYQQLLARMPAQPDALHGLGLLLYRRGNLRDALTRLAQARAADARNPVYCFNHGVVLQ
                     RAGLLPDAVDAYNRAIQLNPRYIDPRTNLGNVYKELGRLAEAQATYEQVLALNPDHAE
                     AHNNLGVVLKEQGR"
     assembly_gap    2688..2787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2788..3742
                     /locus_tag="PK589_15380"
     CDS             <2788..3742
                     /locus_tag="PK589_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21043.1"
                     /translation="CFAETAKAKCAHGRPVTDSAVFRSRVKHDAEQIGYLFERGLIGE
                     EWRGYHDALLQLCGRLEPAPGTVSANRVALSPADLQPIAPSFNRLLYIAPCDTIAEGA
                     LAPDLDHAAIEARYLATQPEVTYIDGLLSEEALRRLREFCWTSTIWKKDYENGYIGAF
                     LGDGFASPLLLQIAEELRRRLPRIFGTHRLTQAWAFKHDSARRGLNIHADAAAVNVNF
                     WITPDDANLNPESGGLVVWDKEAPREWDFKTYNSDKARGSIYEWLTSQGAKEIKIPYR
                     ANRAVVFNSDLFHETDDIAFKEGLTNRRINITLLYGHRHRP"
     gene            complement(3708..5834)
                     /locus_tag="PK589_15385"
     CDS             complement(3708..5834)
                     /locus_tag="PK589_15385"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNJ21044.1"
                     /translation="MSQTTPKILIADDDPLARLFVKNALEPAGMIVLEATGGKDALTK
                     FEAIGPDLVILDIMMPDMDGYLTCSRIRSLPRGKRVPILVLTGLDDAQSIGQAYQHGA
                     TDFITKPVNATILCHHVRYMLRTNNVLHALIRSESRLELAQRIARIGNWDWNPKTNRF
                     AMSNELCRLVGVRPQDFAGTFEAFLALVHADDRSIVTGALQKLVAQHTPCDIDHRIVL
                     PNGTDFVIHLQAEGVREEETDEITVIGTAQDITERKQAERAIHQLAYYDSLTGLANRV
                     LFKDRLSNAISYASRHQQHLATLFIDLDRFKIINDTLGHTVGDRLLTHVAERLSESVR
                     QSDSVSRHADQEPMHALARLGGDEFTILLTALPQPEDAGRVARRILESLAHPFSIEGH
                     EIFISASIGISIFPSDGSTVEALLKNADTAMYHAKEQGRNNCQYYSSGLNAAAAERLD
                     LENELRRALEREEFVVFYQPKLNIHSRRILGAEALVRWKHPKRGLVPPGVFLNAAIDT
                     GLIRSMDEWVLREACRQVKAWEKAGLPPITISANVSNSLFHGRTLPTTVADALRDSGL
                     NPAQLELELTESIAMRDVEASVTMLEGLRTMGVRLSIDDFGTGYSSLSYLQRFPLSRL
                     KIDQSFVRDLLTNENNVKITRAIIAMAHSLNLSVLAEGVETEGQLAKLREEGCDEVQG
                     YLFSRPVCAEDFEKLLKGDADARTAA"
     gene            complement(6058..6786)
                     /locus_tag="PK589_15390"
     CDS             complement(6058..6786)
                     /locus_tag="PK589_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21045.1"
                     /translation="MADASASRLPRTWLPALLVSIIISTLCAAALYVLLAQHDRSLVA
                     QRNASAVMAAEYSARATAQTLAWFSESMGGDNFDSIQQTLEQHAQQANLLAAAVIMDN
                     NVVVASSNPAAIGTTLQDAASLAARRTQSGVITSGIEQGRAAFIVMEPFRRDNRVAGW
                     IRLAVATPPEAAAPRSDDDLGRDVALVIAPLLLLTATLLMLTMRGLMSRVRTLLAGIV
                     LEAREQGPHTIPGGLDTTERGEMA"
     gene            complement(6815..7201)
                     /locus_tag="PK589_15395"
     CDS             complement(6815..7201)
                     /locus_tag="PK589_15395"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNJ21046.1"
                     /translation="MSSFTQRRLTMEQRQHERFPAHFRSAFSSVNRVEGDGTVVDLSL
                     RGCGIVSQTAMHPGTTLTLRIHLPDPETMLTVQQAVVRWCRDGRIGLEFQSLPPDEWA
                     RLQRIVKALTRHPYERTQDAQEGSGA"
     gene            complement(7257..8708)
                     /locus_tag="PK589_15400"
     CDS             complement(7257..8708)
                     /locus_tag="PK589_15400"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNJ21047.1"
                     /translation="MTLNTDMQVNRIDDYLWEIPPSEKVGMLVPARIYASPAILGAMD
                     RGVFDQVTNVACLPGIHRYALCMPDGHWGYGFPIGGVAAFDPEDGIISPGGVGYDINC
                     GMRLIRTDLTRAEVQPKLELLMTELFRRVPAGVGSRGFVNVNRREFREVMRKGAVWCV
                     AKGYGWDEDLERIEERGCLDGADPAHVTDYAIERGINQLGTLGSGNHYLEVQVVSDKN
                     IFDRETAAAMGLTGRDQVVVMVHCGSRGFGHQVASDYLKVFEKAMRRYGISVKDQQLA
                     CAPFRSPEGQEYFGAMNCAANTAFANRQVITHQIREAFAAVFDSTPHALGMHLIYDVA
                     HNIAKVERYGDGEWLVHRKGATRAFGPGSPELPDCYRAIGQPVICGGSMETGSYLLVG
                     TNRAMHDTFGSTMHGSGRTMSRAQAKRSVRGEQLLRDMKQQGILVKAVSMSGLAEEAG
                     LAYKNISDVVNTVDQAGITKKVAELKPIGNIKG"
     gene            complement(8757..9197)
                     /locus_tag="PK589_15405"
     CDS             complement(8757..9197)
                     /locus_tag="PK589_15405"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HNJ21048.1"
                     /translation="MPGTFRFLDDIAMADMAFEAEGDSLASLFDAATQALLQSLADPA
                     TVAQTWQQVVDMEEPDIDTLLFEWLNRLVYLKDAQAVVFHHATLTVQAAPGQSAWHLH
                     AELVGAPVDQATQDLRSDIKGVTKHLYAVTQTGNSWKARVVLDV"
     gene            complement(9203..10177)
                     /locus_tag="PK589_15410"
     CDS             complement(9203..10177)
                     /locus_tag="PK589_15410"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNJ21049.1"
                     /translation="MRLTFTIQRFNPETDQQPHQEEYRLDIGRGMTVLEALIRIKNEL
                     DGRLALRYSCRSAICGSCAMLINGTQKLACRTSIRKELERHGRISIAPLPNLPLIKDL
                     VVDMAPFWEKIRAVTPWLTPITHPTKRYGSSGQLRLLPDSYQFHNVDACIMCAACVAA
                     CTSHEVSRGFLGPAALAKAARFVADPREPADAKQVRLAALQEADGIWDCTRCNMCVQV
                     CPKDVQPMEAIIRLRRSSLRHGLTTAEGARHITGFVDLVRQEGRLNEALMPLKVLGFN
                     LQRVVDVMPLGVRMFFKGKVPLPFGHTIPGIEQVRAIFAAARRRTTRV"
     gene            complement(10222..11379)
                     /locus_tag="PK589_15415"
     CDS             complement(10222..11379)
                     /locus_tag="PK589_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNJ21050.1"
                     /translation="MSHSTDQHTNPVPGATVFIAASETDSNLYYATKFIAPDPFIYLE
                     VKGERLMIMSDLEVDRARHQATVDRVVSYSELERRAKAQGVKDPGNIDVIHLVLQDAG
                     VRDILVPPTFPFLHAQRLQELGYRLRTKRDPFYESRVVKSDEEVRHIEAAQRATEKAV
                     AAAHESLRRAEIRDNVLWLDGQVLTSERVKKLINVALMECDCVAQHTIVAGGEQACDP
                     HDEGSGPLPAHRSVIFDVFPRSADSRYFADMSRTVVRGQPSPELKKLYQTVKDAQEEA
                     ITKIRDGADGAVIHQGICDRFEKSGYKTGVVNGRMQGYFHGTGHGVGLDIHEAPRISR
                     TGSLLQEGHVVTVEPGLYYPGLGAVRIEDMVLVTKDGCRNLTNYPKVFELG"
     gene            complement(11431..14844)
                     /locus_tag="PK589_15420"
     CDS             complement(11431..14844)
                     /locus_tag="PK589_15420"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HNJ21051.1"
                     /translation="MRVRPRTVVLSLLGLVVAGILLLFYSRELFGVDILKNFFLQQLE
                     TSLRRKIEVDRIKLVVLPSIRLELTNVGIYGHDDPTHVVFSAKEIDIVLRLLPLLKKQ
                     VVAKRIFLNEPTVTLVRNRSGHWNVLAGMPSAAKDESAYQMFSRLLQIREATIQKGHI
                     TVTDEARPDGVRTTTLETVEMALKVYPGKAEGDLHISAALPANGAPSSFSLTGTIGLS
                     ESSSTLAAEEPYSVRPAFQFEGEIDTTNLRLREAADFFGPRPVPPQLQGGASLQSRIR
                     VAPGVAGYDVVLSEIAANVDELAVTGKANLAGLLTSQPTFSITFAAPSIDLRSLFTRI
                     PAQWIHAQLPAIIEQRQLGGTVEILSATLTGATAPSPQLSLTGDFRIDKGMALIGNDR
                     VPTQNLAATVSVEPGRIRVGKVTGAYGSLQITDGKALVSFLDDGPWMELDVSGDMSAA
                     DLVKFLTKTIRADKLTSLLAQSRDIEGQTHPTFRLVGPLDKPEGITFAGGEVLAEQVS
                     LMNPSLPQRLTAMRGRIVFSQTGGAQFDQVTANVGEAQLQFNGMISGGTPSVFQDFVI
                     RAKGSASQLRQMVSAGTFPDDLLYGMVNARVQLSGPSGAPHLRGEIGLNEAKLVLPTL
                     GEKPLGSPASLELDADVSKGIGLVISRLELVVPPLRLPLKGRITLGDQFSIDASLATG
                     TVSVSSLPDWVYRSGFEAGNLEVSMDVKGSDAEWKNWRAGGWLALTNGLMTLKGVDGA
                     VEDIYLRLKFSKNIADIKQLSFRIKDSDVSLSGALKNWTTKPVIAVKIESAQMDLDLL
                     IPKGHRSPIREFLETLASTSQVSATATIEKGLYKHLRFGGLSGRLTIQDGMLDLDRVV
                     AQSGTGHAAGRMVVRLPKGEPAETESSVRMTGIPAEAVLQLLGAHDQPVTGDMKLTGA
                     LRGHGRNPHGVLPTLNGKVELVLQDGRILKTEKRAIWKILSILNLPAVLQGKVDLEKE
                     GLQYNRASATLTVQNGLVKTQNIILDSAVLKISAVGTYDMPTDQLDMIWAVSPFGSYS
                     QFLKSIPLFGRLVAGDRKGLATALFQVKGSIDDPQVTYMPMKSFTTGLTGVAQLAFDL
                     LKNTVMLPIDILAPQEEKEPLFDPSLEIQTPPSAPPPVDVPAATAPAAP"
     gene            15003..16289
                     /locus_tag="PK589_15425"
     CDS             15003..16289
                     /locus_tag="PK589_15425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ21052.1"
                     /translation="MRRPLNGGTLGESRVGLDTSAVRPRWGILALLFAISAVTYMDRV
                     NISVTARQMMPAYGLTDQDMGYVFSAFVFGYALCQIPGGRLGDRWGARVVLACALLWW
                     SLCTVLTAVVATLPVAGLVGTVGALVIVRFLLGVGESVALPNFNRAVADWMPPGQRGL
                     GIGIAIGGIGIGAAITPPLASWVMVNYHWQSVFYLSALIGLMVALLWVLFSRDGRSDA
                     TAKADTIPTPVPWGTIFRSRPLWWLVASYTCLGYVAYIYMSWFYLYLVNVRGIDLLRG
                     GWLAAGPFLAILLFCPLGGWVTDKLVPTIGLRRARLSIGMLGMALAGGLIAVGAWVES
                     QTLAIVCLSLGAGWLYFTVGAYWSVTTDLSKTHAGTLSGVMNTGANVGGVISPSLTPW
                     LADHWGWTASLLVAAGIALCGGLMWMKVNPEDGLRE"
     gene            complement(16321..>17427)
                     /locus_tag="PK589_15430"
     CDS             complement(16321..>17427)
                     /locus_tag="PK589_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HNJ21053.1"
                     /translation="LFEPIQVGGLTLSNRIVIAPMCQYSAVDGHMTDWHLIHLGHLAL
                     SGAAALTIEATAVEPEGRITYADTGLWNDATEGAMAHTIESIRRWSDIPLIIQLAHAG
                     RKASTDVPWEGGEQFPPSHNHGWQTVAPSPIPFASGEHPPKALDHTAMARIRESFAAS
                     AQRAGRLGFDGVQIHVAHGYLLHEFLSPLSNCREDEYGGRLEHRMRFPLEVFDAVRAA
                     FRSDRPVTVRVSGTDWVEGGWDIEQTIVFAKALEARGCAGIHLSSGGLDPRQQIPVGP
                     GYQVPLARAVKQAVSMPVIAVGLITDYDQAEEILSRGDADMIALARAILYDPRWPWHA
                     AAHLGGQVKAPKQYWRSQPSRYRDLFLKEHGEAR"
BASE COUNT         3259 a         5135 c         5375 g         3558 t
ORIGIN      
        1 attgccaatt ccccggcgta tatgacccgt aatccggtgg tttccctcca gcatgccgtg
       61 tccatgctgg gcatgaacct gatgtccgaa ttggccttct ctgcctccat caagggcagc
      121 gcattcaaag tgccgggatg ggatggtgaa gtcaagagtc tctggcatta ttccctcgcc
      181 agcggagcct atgccaaaga aattgcccgc atgcgccgat tcaatgtcga aagtgcctat
      241 ctctgcggtc ttttgcatgg catcggccgg cccgtcgtgt tgcagacgct ggtcgccctg
      301 gcgaaggagc aaggcagtcc attgaccaag gagttgctcc atcagctgct cgacggatat
      361 tacatccaag tgggcctgct ggttgcggac gaatggggcc ttccgccgcc cgtggtggaa
      421 tcaatcgggt tccacgccga ttatcattac gccaaaacgg cgaagcagga atgtatgacg
      481 acctgcctgg ccggtcgctt ggcgaaacat tttctggatc ctgagggctt cgatgaagcc
      541 accgtacgag accattcggt ctttgccgac ctgaacctgt accccaaaga tatcgatgcg
      601 ctcctggccc ataaggacaa ggtccaagcc gtcgtggagg cgatgccact gtgatgccgg
      661 cgaccgccta tgacatcgtt gtcgttggca gcggaccagc gggccagaag gccgcgatcc
      721 agggtgccaa ggcaggcaaa aaggtcgtgc tgatcgagca ggagccgggc atcggcggaa
      781 attgcgtgta tcgggggacg atcccgagca agaccctgcg cgagacggcg gtgcagttcg
      841 agcgcctgaa acgatcgagt gaagtcttcg aaggcaaaat gcgcatcgat gtgccgatga
      901 gcgtcttgct gcatcgcctc gacgaagtgg tgaaagcgca tgagtgttat atggccagtc
      961 agctcacgcg caacggcgtg acctaccggc acggacgcgc gcggctgctc tcgccccacg
     1021 aagtgcagct ggaaaccgtc gatggcgcct gccaaactct caccgccgaa acgatcgtgc
     1081 tcgccacggg atcccgaccg tcgtcgattc ccgagattcc ggtggaccat gaacatgtgc
     1141 tggatagtga ttcgatcctg tcaatgattt acctgccccg ttcgttgacc attctcggtg
     1201 gcggtgtcat tgcctgcgaa tatgcgtcga cttttgccct gttgggcgtc gaggtcacca
     1261 tcatcgaccg agccaaacgt cccctcccct tcatggatca cgatatcgtc gacgtgtttc
     1321 agcgcagcat cgagcgtcaa gggggacggt tttattccgg ccatacggtc accgaggtgg
     1381 cctgggatgg tgtctcctcc gtggtggccc ggctcgcgaa cggtatggca gtcaaaagcg
     1441 aaaaaatgct ggtggcgctg ggccggcaac cgaatgtcga ggaattgaat ctggaggcgg
     1501 cgggattgtc gctcgatgaa aagggacata tcccggtgaa cgatcacggg cagactgcag
     1561 tgccgcacat ttttgcggcc ggcgatatgt tgggccgccc gcccgccttg gcttctcagg
     1621 ccatggaaga cggccgacgc gctgtcagcc atgctttggg tctgccggtc ggagattcgc
     1681 tcaatcaggt gcccatcggc atctatacga ttccggaaat cgcttccatc ggtctcgatg
     1741 aggaacaggc ggcggcccgt taccggggtc cgatcatcgg ccgcgcgccg tttacggaaa
     1801 ttgctaaggg ccagatcacc ggcgcctgcg acgggttgtt gaaacttgtc tccgatccct
     1861 ccggcgaacg cctgttaggt gtgcaaatcg tgggcgagaa tgcgacggaa ctgattcatc
     1921 tcggccagat ggctctgcag gatggggcga ccatcgatcg gttcatcgac accattttca
     1981 gctttcccac gtttgccgaa gcctatcgcg tcgccgccct cgacattctg ggccagcgcc
     2041 gtaaacggcg ggacgaggct caagcagcct gatcgtccat ccttctctcg ccgcttcctt
     2101 tgacccccgt cccccatctg cgctactctc tccatcggcc ggtcttccga tcgatggagg
     2161 ttgtacatga atcgtgagga gcgtcgccgc gaagaaaagc tacaggggcg caatgccgga
     2221 ggcccggcag cggcgttggc cgggttgcgc gaggcccaga tgcatcatca ggccggccgc
     2281 ctggatgacg cagaacgtgg ctatcagcag ctgctggcac ggatgccggc gcagccggat
     2341 gccctccatg gtttaggcct gctgctgtac cggcgcggca atctccgtga cgcgctcact
     2401 cgcctggccc aggcacgcgc ggccgatgcg cgcaatcccg tctactgctt caaccatggc
     2461 gtcgtgcttc aacgggcagg gcttctccct gacgcagttg atgcatacaa tcgagccatt
     2521 cagctcaacc cccggtacat cgacccccgc acgaacctcg ggaacgtcta taaagagtta
     2581 gggcgcctgg cggaggccca agctacctat gaacaggtgc tggcgctcaa tccggatcat
     2641 gccgaagcgc acaacaatct tggcgtcgtg ttgaaagaac aaggccgnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnctg ctttgcggaa acggccaagg ccaagtgtgc
     2821 acacggccgc cccgtcaccg actccgcggt ctttcggtcg cgtgtgaagc acgatgccga
     2881 gcaaatcggc tacctgtttg aacgcgggtt aatcggcgag gaatggcgcg ggtatcatga
     2941 cgcgcttctc cagttgtgcg ggcgtctcga gccggccccc ggcaccgtct cagccaatcg
     3001 tgttgccctc tcccctgccg accttcagcc tatcgctcct tcattcaacc ggctcctgta
     3061 cattgcgcct tgcgacacga ttgccgaagg ggcgctggcg ccggatcttg atcacgcggc
     3121 gatcgaagca cggtatctgg cgacgcagcc ggaggtgacc tacatcgatg ggctcctgtc
     3181 ggaggaagcc ctgcggcgtt tgagagaatt ctgctggacg tcgacgatct ggaagaagga
     3241 ctacgagaac gggtacatcg gggcgtttct cggcgacggc tttgcgtcgc cgctgttgct
     3301 ccagattgcg gaagaacttc gccggcgtct gccgcgtatt ttcggcacgc atcgtctcac
     3361 ccaggcctgg gcgttcaagc acgatagcgc ccggcgcgga ttaaacatcc atgccgatgc
     3421 cgcggcggtg aacgtcaatt tctggatcac gccggacgac gccaatctca atcccgagag
     3481 cggcgggctg gtggtctggg acaaggaagc cccgagggag tgggacttca aaacctacaa
     3541 tagcgacaag gcgcggggca gcatttacga gtggctcacg tcgcaagggg cgaaggaaat
     3601 caagattccc tatcgggcca atcgagccgt ggtgttcaat tcagatctgt ttcatgaaac
     3661 cgacgacatt gcgttcaagg aaggactcac caaccggcgg atcaacatta cgctgctgta
     3721 cgggcatcgg catcgccctt gagcagtttt tcgaaatctt cggcgcaaac agggcggctg
     3781 aacagatatc cctgcacctc atcgcatcct tcttcccgta acttcgccaa ctgaccttcc
     3841 gtctccacgc cttcggccag caccgacaga ttcaagctgt gggccatggc gatgatggcg
     3901 cgcgtgatct tcacattgtt ctcgttcgtc agcaaatccc ggacgaagga ttgatcgatt
     3961 ttcaaacggc tcagcggaaa ccgttggaga taactcaacg aggaataccc ggtgccgaag
     4021 tcatcgattg agaggcgcac ccccatggtc cgcagcccct ccaacatggt gacggaggct
     4081 tcgacgtccc gcatcgctat ggactcggtc agttcgagtt cgagttgcgc gggattcaag
     4141 ccggagtccc gcagcgcatc agcgacggtg gtaggcagcg tacgcccgtg gaagagcgag
     4201 ttggagacat tggccgaaat ggtgatcggg ggaagtcccg ccttttccca ggccttgacc
     4261 tgacgacagg cctcccggag cacccattcg tccatggagc ggatcaagcc cgtgtcgatg
     4321 gcggcgttca agaacactcc cgggggaacc aggccgcgct tgggatgttt ccatcgcacc
     4381 agcgcttcgg ctccgaggat gcggcgcgag tgaatattca gtttcggctg atagaagacg
     4441 acgaactctt cccgttccag cgcgcgccgc agttcgtttt cgagatcgag gcgttcggcc
     4501 gccgcggcat tcagtcccga cgaatagtac tggcagttgt tccggccctg ttccttcgca
     4561 tggtacatgg ccgtgtcggc gttcttcagg agcgcctcga ccgtggagcc gtccgatggg
     4621 aagatcgaaa tgccgatgct ggcagagata aagatttcat ggccctcgat gctgaacgga
     4681 tgggccagcg attccaggat tcgccgggcg accctgcctg catcttccgg ctgcggtaag
     4741 gccgttaaca ggatggtgaa ttcatctcca ccgagacgcg cgagggcgtg catcggttcc
     4801 tggtcggcat ggcgactgac cgagtcactt tgccgaaccg attcgctgag acgttccgcg
     4861 acatgtgtca gcaaccgatc gcccaccgta tgtcccagcg tgtcattgat gatcttgaag
     4921 cgatccaggt cgataaacag ggtcgccaga tgctgctggt ggcgcgatgc ataggagatg
     4981 gcattcgaca gacgatcttt gaacaacacg cgattggcta ggccggtcag actgtcgtaa
     5041 taagcgagct gatgaatggc ccgctccgct tgcttccgct cggtgatgtc ctgggccgtc
     5101 ccgatcacgg tgatttcgtc ggtctcttct tcgcgcacgc cttcggcttg gagatggatc
     5161 acgaagtcgg tgccgttggg cagtacgatg cgatgatcga tatcgcaggg cgtatgttgc
     5221 gcaacgagtt tctgcaacgc acccgtcacg atcgaacgat cgtccgcgtg cacgagggcg
     5281 agaaacgcct cgaaggtacc ggcaaaatcc tgcggccgca ccccgaccag gcggcacaat
     5341 tcattcgaca tggcgaaacg gttggtcttc ggattccagt cccagttgcc gatgcgagcg
     5401 atgcgttgcg ccagttccag ccgggattcg ctgcggatca acgcgtgcag cacgttattg
     5461 gtgcgaagca tgtatcggac gtggtggcag agaatcgtgg cattgaccgg tttggtgatg
     5521 aagtcggtag cgccgtgctg gtaggcctgt ccgatggact gcgcgtcatc caggccggtg
     5581 aggaccagaa tgggcactcg tttgcctcgc ggaagcgagc ggattcggga acaggtcaga
     5641 tatccgtcca tatccggcat catgatgtcg agaatcacga gatccggccc gatggcttca
     5701 aacttcgtca gcgcgtcttt cccgcccgtc gcctccagga cgatcatgcc ggccggttcc
     5761 aatgcgtttt tgacgaacag ccgggcgagt ggatcatcgt cggcgatgag gattttggga
     5821 gtcgtttgac tcatatggtg tctggcctat aagtctcgcg gtcggttgat tcgcgtcgcc
     5881 gctccggggc acaggccttg tgcgagcagt ggccggcctg ccgattcagc gttccccaca
     5941 gcctatcaaa ctttggaaga actgccagag aattagcact tgtcgagcat ctcgcgtgca
     6001 ggtcgaattc gtaggtgaaa gcctgagagg aacggtatag ccggaatggt tcaggtttca
     6061 cgccatctcg cctctctcgg tcgtgtcgag gcccccgggt atcgtgtgag ggccttgctc
     6121 tctggcttcg aggacaatgc cggccaagag cgtgcggacc cggctcatca ggccgcgcat
     6181 cgtcagcatc agaagagttg cggtcaacag cagcagcggg gcgatcacca gggcgacatc
     6241 ccggcctaaa tcatcgtccg atcggggagc ggccgcttca ggcggtgtcg cgacggccag
     6301 tcgtatccaa ccagccaccc ggttgtcccg gcggaatggt tccatgacga tgaaggcagc
     6361 tctcccctgt tcgataccag acgtgatgac accgctttgt gtccggcgtg ctgccagcga
     6421 ggccgcgtct tggagcgtgg ttccaatggc ggcgggattg ctcgatgcga ccaccacatt
     6481 gttgtccatg atcacggccg cggcgaggag attggcctgc tgagcatgtt gctccagtgt
     6541 ctgctggatg gagtcgaaat tatcgccgcc catcgattcg ctgaaccagg ccagggtttg
     6601 cgccgtggcc ctggcgctgt actctgccgc catgacggcc gaagcattcc gctgcgccac
     6661 gagggagcgg tcatgctgag ccaacagcac atagagggcc gcggcgcaca atgtggaaat
     6721 gatgatgctg accagcaacg ccgggagcca ggtccgaggc aatcgcgatg cggaagcgtc
     6781 agccatgcta tatcctcctc gacaggaagg ggcgctaggc gccggagcct tcctgcgcgt
     6841 cctgagtccg ttcgtaggga tggcgagtga gtgctttgac gatccgttga agccgggccc
     6901 attcgtcagg cggcagggac tgaaattcaa gaccgatgcg gccgtcccgg caccagcgta
     6961 ccacggcttg ctggacggtc agcatggtct ctggatccgg aagatgaatg cggagggtca
     7021 gtgtggtgcc gggatgcatg gcggtctgac tgacgatccc gcatcctcgc aaggagagat
     7081 cgaccacggt gccgtctccc tcgacgcgat tcactgaact gaaggcactt cggaagtgag
     7141 cgggaaaccg ttcgtgctgg cgttgttcca tcgtgaggcg gcgttgagtg aatgacgaca
     7201 cgctctctgt gctgcgttcc ccggaagccg cgcatgtgtg tcgaatgcat gccgtgctag
     7261 ccctttatat tgccaattgg tttgagttcc gccacttttt ttgtgattcc cgcctggtcc
     7321 accgtgttca ccacgtcgga aatgttcttg taggccagtc cggcctcttc agcgaggccc
     7381 gacatcgaca cagccttgac cagaataccc tgttgtttca tgtcgcggag cagttgttcg
     7441 ccgcgtaccg agcgtttggc ctgtgcgcgg gacatggtgc ggccggaccc gtgcatcgtg
     7501 gagccgaagg tgtcatgcat ggcgcgattg gttccgacca ggagatagga gccggtttcc
     7561 atcgagccac cgcaaatgac cggctggccg atcgcgcgat aacagtcggg cagttccgga
     7621 ctccccggcc cgaaggcccg cgtggcgcct ttgcggtgga ccagccactc cccgtctccg
     7681 taacgctcca ccttcgcgat gttgtgtgcg acatcgtaga tcagatgcat gccgagtgca
     7741 tgcggggtgg aatcgaacac ggcggcgaag gcttcgcgga tttgatgggt gatgacctgg
     7801 cgattggcaa atgcggtgtt ggcggcgcag ttcatcgcgc cgaaatactc ctgcccttcc
     7861 ggtgaacgaa acggcgcgca ggcgagttgt tgatccttga cggagattcc gtagcggcgc
     7921 atcgccttct cgaagacttt gaggtagtcg ctcgcgactt gatggccgaa tccgcgggag
     7981 ccgcagtgga ccatcaccac gacttggtcc cggccggtca ggcccatcgc ggcggcggtc
     8041 tcccgatcga agatgttttt gtctgagacg acctgcactt ccagatagtg attgcctgat
     8101 cccaatgtcc cgagttggtt gatgccccgt tcgatggcgt aatctgtcac atgcgccgga
     8161 tcagcgccgt cgaggcaacc ccgttcttcg atccgttcca ggtcctcgtc ccacccatac
     8221 ccctttgcga cgcaccagac agcgcctttg cgcatgacct cccgaaactc gcgccggttc
     8281 acgttcacga agccgcgcga tcccaccccc gccgggacgc ggcggaacaa ctcggtcatc
     8341 agcagttcca gtttcggttg cacctcggcg cgcgtcaaat ccgtgcggat caaacgcatg
     8401 ccgcagttga tgtcatagcc gacgccgccg ggagagatga tgccgtcctc cggatcgaag
     8461 gccgcgacgc cgccgatagg aaacccgtag ccccaatggc catcaggcat gcagagcgca
     8521 taacgatgga tgccgggcag acaggcgacg ttggtgacct gatcgaagac gccgcgatcc
     8581 atggctccga ggatggccgg actcgcataa attcttgcgg gaaccagcat gccaaccttc
     8641 tcggatggcg gaatttccca gagatagtcg tcgatacggt tcacctgcat gtcggtgttg
     8701 agtgtcatcg gttcacctgt caattgcaca agaattgtga gggatgtggt cgttcatcag
     8761 acatccaaga ccactctggc cttccaacta tttccggttt gcgtcacggc atacaagtgt
     8821 ttggtgacac ccttgatgtc ggatcggaga tcctgcgtgg cctgatcgac cggggccccg
     8881 accagttctg catggaggtg ccaggcggat tgaccgggcg ctgcctgcac cgtcaacgtg
     8941 gcatggtgaa agaccacggc ttgcgcgtct ttcaagtaca cgagccggtt cagccattca
     9001 aagagcagcg tatcgatgtc aggttcttcc atgtcgacga cctgctgcca ggtctgcgca
     9061 acggtagcag gatcggccag gctttggagg agcgcctgcg ttgcggcatc gaaaagcgac
     9121 gcaagagaat cgccctcggc ctcgaaggcc atgtcggcca tcgcgatgtc gtcgagaaat
     9181 cggaaggtgc ccggcatggg cgtcagacgc gcgtggtccg tcgtcgggct gcagcgaaaa
     9241 ttgcccggac ctgttcgatc cctggaatgg tatggccgaa cggcaatggg accttacctt
     9301 tgaaaaacat gcggaccccg agcggcatga catcgaccac gcgttgcagg ttaaatccga
     9361 gaactttgag cggcatcaag gcctcgttca accgtccttc ctggcggacg aggtcgacaa
     9421 agccggtaat gtgacgggcg ccctcggcag tggtcaaacc atgccgaagc gaagaccgcc
     9481 gcagccggat gatcgcctcc atcggttgaa cgtctttcgg gcagacctgc acgcacatgt
     9541 tgcaccgcgt gcaatcccag atgccgtcgg cttcttgaag cgcggcgagc cgaacctgtt
     9601 tcgcatccgc cggttcgcgc gggtcggcca cgaagcgcgc cgctttggcg agcgccgccg
     9661 gaccgagaaa gccgcgggag acttcatggg aggtgcaggc agcaacacaa gccgcgcaca
     9721 tgatgcaggc gtctacgttg tgaaattggt aactgtccgg cagcaggcgc agttgccctg
     9781 aggatccgta gcgtttggtc gggtgcgtga tcggggtaag ccagggcgtg accgcccgga
     9841 tcttttccca gaagggcgcc atatcgacca ccagatcctt gatgagcgga agattcggca
     9901 agggagcgat gctgattctg ccatgccgct ccagttcttt ccggatggac gtacggcagg
     9961 ccagcttttg tgtgccgttg atcagcatgg cgcaggagcc gcagatcgcc gaacggcagg
    10021 aatagcgtag cgccaggcgg ccgtcgagtt cgttcttgat gcggatcaag gcttcgagca
    10081 ccgtcatgcc tcgtccgatg tcgaggcggt actcttcctg gtgcggctgc tggtcggttt
    10141 cgggattgaa tcgctggatc gtgaaggtga ggcgcataac gttgtgaacg tcgagatgcg
    10201 aataccgtag gcggtagacg cctaaccgag ttcaaacact ttcggatagt tcgtcagatt
    10261 ccggcaaccg tctttcgtga cgagcaccat gtcttcgatg cggacggcgc caagtcccgg
    10321 atagtagagc cccggttcca ccgtgacgac atggccttcc tgaagcaatg aaccggtccg
    10381 gctgatccga ggcgcttcat gaatgtccag tcccacgccg tgcccggtgc cgtggaagta
    10441 gccttgcatg cgaccgttca ccaccccggt cttgtagccg gatttttcaa accggtcgca
    10501 gataccctgg tggatgaccg ccccgtcggc cccgtcgcga attttggtga tggcttcttc
    10561 ctgcgcgtcc ttgacggtct gatacaactt cttcaactct ggagagggct gcccgcgaac
    10621 cactgtccgc gacatgtcgg caaaatagcg ggaatcggcg gagcgcggaa acacgtcgaa
    10681 aataacgctg cgatgcgccg gcaacgggcc gctgccttcg tcgtgcggat cgcaggcctg
    10741 ttctccgccg gccacgatgg tatgttgcgc cacgcaatca cattccatca gggccacgtt
    10801 gatgagtttc ttcacgcgtt ccgatgtgag cacctgcccg tcgagccaaa ggacattgtc
    10861 gcgaatctcg gcgcggcgca agctctcgtg ggcagcggcc acggccttct cggtcgcgcg
    10921 ctgggctgct tcgatgtgcc ggacttcttc gtctgacttc accacccggc tttcataaaa
    10981 cggatcgcgt ttcgtgcgta gccggtatcc aagctcctgg agtcgttgcg catggagaaa
    11041 gggaaaagtc ggtggcacca ggatatcgcg gacgccggcg tcctgcagga ccagatggat
    11101 gacatcgata ttgccgggat ctttgacgcc ctgtgctttc gcgcgtcgct ccagttcgga
    11161 ataggacacc acgcgatcaa ccgtcgcttg atgccgcgca cgatcgacct ccagatcact
    11221 catgatcatc agccgctccc ctttcacctc aaggtagatg aagggatccg gcgcgatgaa
    11281 tttcgtcgcg taatagagat tggaatcggt ttcgctggcg gcaatgaaga ctgtcgcgcc
    11341 ggggacggga ttcgtatgct ggtcggtgga atggctcatg aggtcgaggc gatctaatca
    11401 atcggatgct atcacgcgct ccggaggtgg tcaaggcgcg gccggcgccg tcgcagccgg
    11461 aacatcgaca ggcggcggtg cggagggcgg tgtttgaatt tccaatgacg gatcgaacag
    11521 cggctccttc tcctcttgcg gtgcaagaat gtcgatcggc agcatgaccg tattcttcag
    11581 caggtcgaag gcaagctgcg ccacgccggt cagacccgtg gtaaaggact tcatcggcat
    11641 gtacgtgacc tgtggatcat caatcgaccc cttcacctgg aacagggccg tggcgagacc
    11701 tttacgatcc cctgccacca accgcccgaa gagcggaatc gatttcaaga attgtgagta
    11761 tgatccgaac gggctgaccg cccagatcat gtcgagctga tccgtgggca tgtcgtaggt
    11821 gccgacggcc gaaatcttca gcaccgcgct gtccagaata atgttctgcg ttttgacgag
    11881 cccgttttgc accgtcagcg tggcgctggc gcgattgtac tgcaggcctt ccttttcgag
    11941 gtccactttc ccttgcagga cggcgggcag attcaggatg gagaggattt tccagatggc
    12001 gcgtttctct gttttgagaa tgcgtccatc ctgtagcacg agttccactt tgccgttgag
    12061 tgtcggcagc actccatgag gattgcgtcc atgtccgcgc agcgccccgg tcagtttcat
    12121 atcgccggtg acgggctgat cgtgcgcacc caacaattgc agcacggcct cggcgggaat
    12181 gcccgtcata cggacagagg attccgtttc agccggttct cctttgggaa ggcgcaccac
    12241 catccgccct gccgcatgtc ccgttccgga ttgtgcgaca acccggtcga gatccaacat
    12301 gccgtcctga atggtcaacc ggcccgacaa tcccccgaac cgcaaatgtt tgtagaggcc
    12361 cttttcaatc gtggctgtcg cgctgacctg actcgtggag gcgagtgttt ccaggaattc
    12421 ccggatcggt gagcgatggc ccttcgggat gagcagatcc aggtccattt gcgccgactc
    12481 gattttgacc gcgatcaccg gcttggtcgt ccaattcttc agggcgccgg acaggctcac
    12541 atcgctgtcc ttgatgcgga acgaaagctg tttgatgtcg gcgatgtttt tggagaactt
    12601 caagcgaagg tagatgtctt ctaccgcgcc gtccacgccc ttgagcgtca tcaggccatt
    12661 ggtcagcgcc agccagccgc cggcgcgcca gtttttccac tcggcatccg aacctttcac
    12721 gtccatggac acttcgagat tgccggcttc gaagccgctg cgatacaccc agtcgggaag
    12781 actggagacc gataccgtgc cggtggcgag cgacgcgtcg atggaaaact gatcgccgag
    12841 ggtaatgcgt cccttcaacg gcagccgcaa gggcggcacg accagttcca ggcgagaaat
    12901 caccaggccg atgcctttgc tgacgtcagc atccagttcc agggaggccg gcgagcccaa
    12961 cggtttttca cccaacgtgg gtagcaccag tttggcttca ttcaggccga tctctccgcg
    13021 caagtgtggc gccccggacg gccccgagag ctgcactcgc gcattgacca tgccatagag
    13081 caggtcgtcg ggaaaggtgc ccgccgatac catctgccgc agctgcgagg cgctgccttt
    13141 cgcgcggatc acgaaatcct gaaacacgct cggcgtacct ccgctgatca tcccgttgaa
    13201 ttgcagctgg gcttccccca cgttggccgt gacttgatcg aactgcgcgc cgccggtttg
    13261 cgagaagacg atacggccgc gcatggccgt gaggcgttgc ggcagcgagg gattcatgag
    13321 gctgacctgt tcggccagca cctccccgcc tgcgaaggta atgccttcgg gtttgtccag
    13381 cgggccgacc agccggaacg tcggatgggt ctggccttca atgtcgcggg actgcgcgag
    13441 cagcgaggtg agcttgtccg cacgaatggt cttggtgaga aacttcacca ggtcggcggc
    13501 gctcatgtcg ccactcacat ccagctccat ccaggggccg tcatcgagga atgacaccag
    13561 ggctttgccg tcggtgatct gcagtgagcc gtaggcgccc gtcactttgc cgacccggat
    13621 gcgtcccggt tccactgaca cggttgcggc caggttctgc gtcggcacgc gatcattgcc
    13681 gatcagggcc atgcccttat caatgcgaaa atcacccgtc aatgacaact gcgggctggg
    13741 cgccgtggcg cccgtcaagg tcgcggagag aatctcgacg gtgccgccca attggcgctg
    13801 ctcgatgatg gcaggcagtt gggcatgaat ccattgcgcg ggaatccttg tgaagagcga
    13861 gcggagatcg atcgatggcg ccgcgaaggt aatggaaaac gtcggctggg aagtgagcag
    13921 tccggccaga ttggccttcc ccgtcacggc cagttcatcc acgttggcag caatctcgga
    13981 cagcacgaca tcatagccgg ccacgcccgg cgccacccgg atgcggcttt ggagactggc
    14041 ccctccctgc agttgcgggg gaaccggcct tggcccgaag aaatccgccg cttcccgcag
    14101 gcgaagattc gtggtgtcga tctcgccttc gaattgaaag gccgggcgaa cggaataggg
    14161 ttcctcggcc gccagcgtcg aagacgattc actcaatccg atcgtgccgg tcagagaaaa
    14221 cgaagagggc gcgccattcg ccggcagcgc ggcggaaata tgcagatccc cttctgcctt
    14281 acccggatag accttcagcg ccatctcgac cgtttcgagc gtcgtcgtcc gcacgccgtc
    14341 cggccgtgcc tcatccgtca ccgtgatgtg ccccttctgg atggtcgctt cacggatctg
    14401 cagcagccgg ctgaacatct gatacgccga ctcgtctttc gccgccgagg gcatgcccgc
    14461 caggacattc caatggcccg atcggttgcg gacgagcgtc acggtcggtt cgttgagaaa
    14521 gatgcgcttc gcgacgacct gctttttcag cagcggcagc aggcgcagga cgatatcgat
    14581 ttccttggcc gagaagacga cgtgtgtggg gtcgtcgtgg ccgtaaatgc cgacgttggt
    14641 gagctccagg cgaatgctgg gcagcaccac gagtttgatg cgatcgactt cgattttacg
    14701 gcgaaggctg gtttcgagtt gctggaggaa gaagttcttg aggatgtcga cgccgaacag
    14761 ttcgcgcgag tagaagagaa gcaggatccc cgcgaccacc aggccgagca gcgacagcac
    14821 taccgtgcgg ggacgaaccc tcactgaccc tcctctaagt agagcaagaa cgcagtgtac
    14881 tggatgcgta cgcgggaaat ccatacacag gcgtgcggca gaggcgcaaa acctgagcga
    14941 gacacctccc atgtcgcggt gcctcgcctt tcacatttca gtcgtgcgtg tacggggcta
    15001 taatgcgccg gccgctgaac ggcgggacac tgggggaatc acgagtgggg ctcgacacat
    15061 cagcggtgcg accacgttgg ggcattctcg ctctactgtt cgcgatcagc gccgtcacat
    15121 acatggaccg ggtcaacatt tcggtcacgg cccggcagat gatgcctgcc tacggactca
    15181 ccgaccagga catgggctac gtcttctcgg ccttcgtctt cggctacgca ctctgccaaa
    15241 ttcccggcgg acgcctgggc gatcgttggg gagcacgagt ggtgctggct tgtgccttgc
    15301 tctggtggtc gctctgtacg gtgttgaccg ccgtggtggc caccctgccg gtagccggtc
    15361 tggtcggcac ggtcggcgcg ctggtcatcg tacgattcct gctcggcgtg ggcgaatcgg
    15421 ttgccctgcc gaatttcaat cgtgctgtgg ctgactggat gccgccggga cagcgtgggc
    15481 ttgggatcgg catcgccatc gggggcatcg gcatcggtgc ggccatcacg cctcccctcg
    15541 cctcctgggt catggtgaac tatcactggc aatcggtgtt ttatctctcg gccttgatcg
    15601 gtctgatggt ggccttgctg tgggtgctct tctcgcgaga tgggcggagc gacgcaacgg
    15661 caaaggcgga caccattccg acgccggtcc cctgggggac aatcttccgc tcccggccgc
    15721 tctggtggct cgtcgcgagt tacacctgtc tcggctacgt cgcctatatc tacatgagct
    15781 ggttttatct ctacctggtg aatgtgcgcg ggatcgatct cttgcgcggc ggctggctgg
    15841 cggcaggacc ctttctggca atcctgctct tctgcccgct cgggggatgg gtcacggaca
    15901 agctggtccc gacgatcgga ttgcgcaggg cccggctttc catcggtatg ctcggcatgg
    15961 cgttggccgg tggattgatt gccgttggtg catgggtgga atctcagacc ttagcgatcg
    16021 tctgcctgtc gctcggtgcc gggtggctct actttaccgt gggcgcctat tggagcgtga
    16081 caacggatct ctctaagacc catgccggga ctctctctgg tgtgatgaat acgggcgcta
    16141 acgtaggcgg cgtgatttct ccgagcctca cgccctggct cgcggatcac tggggctgga
    16201 cggcgtcact actggtggct gctggtatcg cactctgcgg agggctcatg tggatgaagg
    16261 ttaatccgga ggatgggttg agggaatgag ttcttcccct caatgttgca ttctcatctg
    16321 ctatctcgct tccccgtgtt ccttgagaaa taaatcccga tagcggctcg gttggctgcg
    16381 ccaatattgt ttcggggcct tcacctgacc tcccagatgg gctgcagcgt gccacggcca
    16441 gcgcgggtcg tagagaatgg ctcgtgcaag ggcgatcatg tccgcatcgc ctctgctgag
    16501 gatctcttcg gcttggtcgt agtccgtgat gagaccgacg gcaatcaccg gcatggacac
    16561 cgcctgcttg accgcacgag caaggggaac ctggtagccg gggccgactg gaatctgttg
    16621 gcgcggatcg agcccgccgc tgctgagatg aatccctgcg cacccccgtg cttccagggc
    16681 cttggcgaac acaatggttt gctcgatgtc ccagccgcct tcaacccagt ctgtgccgga
    16741 cacacgaacc gttacaggcc gatcagaccg gaatgcggca cgcacggcat caaacacttc
    16801 gaggggaaac cgcatgcgat gttcgaggcg cccgccgtac tcgtcttccc ggcagttcga
    16861 aagcggtgag agaaactcgt ggagcagata tccatgggcg acgtgaatct gtactccgtc
    16921 aaagcccagc cgaccggcgc gctgggccga agcggcgaac gattcgcgga tgcgcgccat
    16981 tgcggtgtga tccagcgctt tggggggatg ctcgccgctt gcaaacggga ttggcgacgg
    17041 ggcgacagtc tgccacccgt gattgtggct gggaggaaat tgttcccctc cttcccaggg
    17101 tacgtcggtc gacgcctttc ggcctgcatg agccagctgg atgatgagag gaatatccga
    17161 ccaacggcgg atgctctcga tcgtgtgtgc catggcacct tcagtggcat cgttccagag
    17221 ccctgtatcc gcataggtga tgcggccttc cggctcaacg gcggtggctt cgatcgtcag
    17281 cgccgccgcc ccggagaggg cgaggtggcc taggtgaatg aggtgccagt cggtcatgtg
    17341 cccatcgacc gccgaatact ggcacatcgg cgcaatgacg atccggttgc tcagcgtgag
    17401 cccgccgact tggattggtt cgaacag
//
LOCUS       DAOQDS010000076        17321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1334_length_17321_cov_21.642071, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000076 DAOQDS010000000
VERSION     DAOQDS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17321
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1334_length_17321_cov_21.642071"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PK589_15435"
     CDS             <1..185
                     /locus_tag="PK589_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21054.1"
                     /translation="IVPMFTWGFRRSNFAFAMIHSFSQNSVFPEPYPYIQVTLRNRQA
                     AAQTSLLWSPRRESNA"
     gene            complement(156..231)
                     /locus_tag="PK589_15440"
     tRNA            complement(156..231)
                     /locus_tag="PK589_15440"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Thr,seq:ggt)
     gene            complement(300..374)
                     /locus_tag="PK589_15445"
     tRNA            complement(300..374)
                     /locus_tag="PK589_15445"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(340..342),aa:Gly,seq:tcc)
     gene            complement(503..588)
                     /locus_tag="PK589_15450"
     tRNA            complement(503..588)
                     /locus_tag="PK589_15450"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(552..554),aa:Tyr,seq:gta)
     gene            complement(825..1772)
                     /locus_tag="PK589_15455"
     CDS             complement(825..1772)
                     /locus_tag="PK589_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21055.1"
                     /translation="MSQFEAIMRCLRSYIDSMQGVAAVMVGGMLLVLLCSAPILAETP
                     AAQPDASRLWSGLVAEAKALRLPTQFLEQVPKHFVTFEFEDLHAFAAEYHPADHRMVL
                     DRSLSLNAAGRTLRPLGRLTHKELETLYHELFHVYMDFLEQEATPSEAHASLIAFARE
                     QQHCRYQQVLITPVLQKRALKEERFLSETESWEVLNETWAVFIGWAVWTQLEVDQKPR
                     RDSRGPAHTSGWIARLDQAEQDAALIGYYEPEDLEEKRMARKRFLAPEFRLSSPELLR
                     LMKDVLGSQPDVIRQATQVLQRSRPVASTRGACAASTKP"
     assembly_gap    1962..2061
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2062..4050)
                     /locus_tag="PK589_15460"
     CDS             complement(<2062..4050)
                     /locus_tag="PK589_15460"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNJ21056.1"
                     /translation="MLYSATAPVMSLTAQSDANMDRDTKPYILKRTPDQDVVRKFSLD
                     YAKELNPQQYAAVTAADGPALVIAGAGSGKTRTLVHRVAYLIDSGVDPSQILLLTFTR
                     KSAEEMLERVGALIGSRSQRVCGGTFHSVANMLLRRYGRVLGIEPSFTIMDRGDAEDL
                     IALLRAQLGLNEKDKRFPRKGTIAEIYSKCENTLRGLEEIVLDEFSHFADHLDALGKL
                     QRAYQLAKRQRQLLDYDDLLVLLRDLMTQDEASRQAISRQFRYILVDEYQDTNRLQAE
                     LIRKLAATHDNVMVVGDDSQSIYAFRGATFRNIMEFPSWFPGATIYKLEENYRSTQPI
                     LSLANEIIQEAPEKYTKHLFTRKLDGPLPALVEAAGENAQSRFVAQKILELREEGVPL
                     NEIAVLFRSSFHSFDLEIELSRCGLPFVKRGGIKFIEAAHVKDLLAHLRVVVNPHDAV
                     SWHRVLMLVEGVGPKKAQDLVAAMVRVTDPYQVLRDSSGRSGKGLKELAVVLENLSKS
                     DDLSPTEQVNRVYEYYLPILKDHHDDYPKRIRDLDHLHTIAESYPGLTEFLADLALAP
                     PDGSAVGVEAAGSDDEQVVLSTIHSAKGLEWQCVFLLWVVDGKFPSVFSFNTDEELEE
                     ERRLLYVAVTRAKRYLFLTYPINVYDRSSGMLLSKPSRF"
     gene            4170..5006
                     /locus_tag="PK589_15465"
     CDS             4170..5006
                     /locus_tag="PK589_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21057.1"
                     /translation="MTTKSHSFAQQMRTVAELMLAVSGESVSVIKRAAPEQALKLKRQ
                     DEWGIYLEFLRAMFNLTDRVSALHIPLREQPQFMDELTDTVIDQLKKVLEPAFGAGDD
                     QMEIVMTIGAAVSESRQTYERYRFLVTEESPAKNDLYRDFSDRVARAVGASGNPKVEA
                     AATLCIAAVIPALIGIFEGKTPAVPADATGNANPSAASSNPRGATGADIKLVSVMSSI
                     KGEEVETRWGLHPRFRQDLTQEEAKQLTATMNRVAKILGERYATVAFSDQWASWHKAG
                     HA"
     gene            5076..7235
                     /gene="glgP"
                     /locus_tag="PK589_15470"
     CDS             5076..7235
                     /gene="glgP"
                     /locus_tag="PK589_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNJ21058.1"
                     /translation="MQAPDHIQPPQDMVPQSLHRLGELARNLWWSWNQPARQLFESID
                     PTLWFLTHHNPVQLLAGVKPERFATLASDPTFARQYSAVLRSFDEYMSSKNTWAATEF
                     PALQNSPIAYFSAEFGLHISIPIYSGGLGILAGDHCKEASDLGIPLVGIGFMYPQGYF
                     KQRINPEGWQEATYVPFNRYDSPIHQALTPAGVPCTIKVQIGHRQVTVLVWQVRNGRM
                     SLYLIDTDVPENTPEDRALSARLYGGDQETRLCQEFLLGIGGVRVLRALGITPAVWHC
                     NEGHSAFLTLERIREFVSTGHSHAEASELVRQSTVFTTHTPVPAGHDIFPFHLMDRYF
                     HNYWGQLGLSREEFLRLGETPESAGHGFNMTALAMRLSAHVNGVSREHGRVSRQMWQH
                     LWPGLAQDLVPIRSLTNGIHAPTWISPESNSLYAKYLSPDWAERIDDPTIWQRVTDLP
                     DDALWAVRQTTRRKLMRFIRERARDGWIRGHLQPMQAIARGTLLDPEALTIGFARRFA
                     TYKRATLLFRDLERLKQLLHNRWRPVQIVFAGKAHPADEPGRYFIHEVLNFCNDHKLG
                     GHIAFLEDYDMHMAKYLVQGVDVWLNTPRAPLEASGTSGQKAALNGVINLSVLDGWWQ
                     EGYNGANGWGIQPLPEGSDTQAQDAHDAEQLFRLLEQEVVPLFYQRDLDGIPRGWLHI
                     VKESIKTVAPRFCTKRMVKEYMRQLYAPATARTPNKW"
     gene            7256..8641
                     /locus_tag="PK589_15475"
     CDS             7256..8641
                     /locus_tag="PK589_15475"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ21059.1"
                     /translation="MPGRYISTWAFPAGLLLMVWISPLPVGAATSTPKTSPSPLERDA
                     MTFYRAAEYSRVIDLIQHPPTGQEPSLEAIRYAILSYVKMGKPEEAWKLYPKLTAGDR
                     PDDPALLREIARAFIVSRVRDSQEHIRIAAYTALAEMGEADTLPLLEDGLLDSSALVR
                     ARAAEAIGRSGLAGRSAALKRALRDEAPGVRIAAINALSDAKVSGITDQLTEIARVDE
                     GPESIFAFAGLYKLGRTDVLTDISSAVTLPDPEVRMAALGVLGRLKRPASLSILSQAV
                     YDPHPAVRAFAAGALGEFGSIEGIAPLTHALGDEDPRVRSVAASSLGRLGVSESRSVV
                     QPLLRDSDEHVRISAVESLLRLGDTGVILNAAALARHPDPSIRGDTAHALALATTANA
                     LPILENLLRDQQPLPRLMAARALGKSPLKSLIPAAKIGLQDSDAAVRITSAGSLLRIL
                     SRKDKVSTRKG"
     gene            complement(9035..9868)
                     /locus_tag="PK589_15480"
     CDS             complement(9035..9868)
                     /locus_tag="PK589_15480"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ21060.1"
                     /translation="MEHTGEKKDQAQAVSPVGPVEDEALTDQVSDQLAASLDSSVGEQ
                     ELIEPMQEVVLEEEKVKDEIDIQIDLLKDPDWVVRREAAITLGEMGDERCVEPLAAAL
                     RDGDWQVREVAIEGIGQIGSPAVDVLLKLLRDWDVRKSAIMALGKIRDERVLEPLMQQ
                     LRNDEFMEDATDALVNLGEPALPGLIKALKDKEELVRKQAVIALGRIKSPEAIDPLIE
                     MLQNKDWFTRLTAAAALEAIGDERGREAIKPLLKDTDMVVKMRVERILAKWKKQPAVV
                     S"
     gene            complement(9932..10132)
                     /locus_tag="PK589_15485"
     CDS             complement(9932..10132)
                     /locus_tag="PK589_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21061.1"
                     /translation="MAKKPTGESDEVRLKKKIAVSTKDESKSGDPAVRSLRKRLKRVQ
                     RKRRALVLRKKHAAGKQAEAKS"
     gene            complement(10264..10923)
                     /locus_tag="PK589_15490"
     CDS             complement(10264..10923)
                     /locus_tag="PK589_15490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNJ21062.1"
                     /translation="MVRNFWNRSRCKTGSALAPLVACLLLSACSALPKGDLPLDLGIK
                     DRGVASWYGKEFHGKLAANGEVFDMTAYTAAHRKLPLGSLVRVVNLANGKSVQVRIND
                     RGPYVSGRMLDLSQAAARELGMLEAGTSPVQIEVIGNHRPVAPIPSSLIASVAGMLLN
                     TDTRVMKRHTRQEEGVEVPVVPLRMMPQEALYVRRERRIGSMLAADHSAHNTVPGLIV
                     S"
     gene            complement(11263..11919)
                     /locus_tag="PK589_15495"
     CDS             complement(11263..11919)
                     /locus_tag="PK589_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21063.1"
                     /translation="MIRQHVVRGMSAPVAVGLLLLMGCSGKGDIIPMNLTPKAKEMTN
                     ASSAVKPMAGPKVVVIPFEDARADHAKVGSRSSMWGGETDFHVSSGSAGEETGQALAD
                     YLKRKGWLVQYQKAAPAESENGPNIVLSGKILDLTVDAKRGFLMTDIEARSKLVIQAN
                     NREDGSSIVRTDAHSGSHDNVFWFDPQDGEELLGEVLEKNFERFVLNTKLEDRSIRFR
                     "
     gene            complement(11976..12341)
                     /locus_tag="PK589_15500"
     CDS             complement(11976..12341)
                     /locus_tag="PK589_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21064.1"
                     /translation="MNQARPSLLGVLVIGAALCVALAGLGSFLTSVYAASNTVHEIKG
                     TVENVHAHEDPPVIVVKGQHGSSEAIVVGAVVKQGATIMRGKKRITLDQIHAGDKVTL
                     KYVKTRDGLTVRSIALHRN"
     gene            12596..13564
                     /locus_tag="PK589_15505"
     CDS             12596..13564
                     /locus_tag="PK589_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ21065.1"
                     /translation="MIEVCNITKRYGHLTAIDRVTFRVEKGEVLAFLGPNGAGKTTTM
                     RILTSFIPATEGTAHVAGFDCAEQPDEVKKRIGYLPETPPVYQELTVAEYLGFVGKLR
                     GLSGTGLTQALERVTERLSLGTVRQRLIANLSRGYRQRVGLAQALIHDPPVLILDEPT
                     VGLDPKQIIEIRELIKSLAGSHSVILSTHILPEATAVCQRVVIINGGRIVAEDTPDQL
                     SARLRKSEKISVTLKAPPVNLAERFREVPGIEHVLTTADPHTVLIECALGRDIREDVA
                     RFAVGQNWGLLEMKPVSMTLEDVFLKLTQREDEPPKSNDTTPGEHH"
     gene            13568..14338
                     /locus_tag="PK589_15510"
     CDS             13568..14338
                     /locus_tag="PK589_15510"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNJ21066.1"
                     /translation="MPPVQAIIRKELRSYFVSPIVYVVGGVFLLTFGFLAYLYIVFTG
                     AQAIQLMQMQGGTAQINLNDLVFRNLFASMRFVLLLILPILTMRLLAEERKLRTFEFL
                     MTAPIRVSEIIVGKFISVYLVFMGMLLLTTLVPLTLALFSDFDWYPVFTGYLGLLLLG
                     GFFLAVGLFASSVTENQIVAAFTSFGLLLMCWLLAGLGSLLGDTPVGQVVSYLSFMEH
                     YDHLVRGLIDTKDLMYYVSGTVLMLFLAHRIVDASRWK"
     gene            14329..15858
                     /locus_tag="PK589_15515"
     CDS             14329..15858
                     /locus_tag="PK589_15515"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNJ21067.1"
                     /translation="MEMTRNPLPIGAIGTGLAIAGVIAYSLVPDKLWLVTLLEGLALA
                     CFIWAFATHFDQVKRFSSQRSTRMGANSAMMVALFLAILAILNFLAARHSIRWDFSEN
                     QNYTLAPETYRVLRNLTRDVNVTVFTREKDPGYQGYKERFDSYRQASPKLTVQFIDPE
                     RQPKVAQNYGVTRTDVAIFESGPQSVRITSPAEVEITGALVRVSKDSKKRVVFLEGHG
                     ERSLEDKDRGGMALTKEVLEKQGYEIGTVTLLQETAVPSNTDVLVIAGPRRAITKEEQ
                     DRIQTYVAQGGHLLLLLDPDTQASMDTLLKIWGLELGAGVLVDLQDRLAQGDLTALLV
                     RTFTDHEITHELTAAVLFPLARHLTFHEEQAKDWDYVPLARTSPRSWAETDMKGRVVS
                     YDEKEDVQGPLALAAALTPKQAPEEGKPRPAVVVVGNSAFASNGFINFVGNSDFFQRT
                     VGWLSEERDLISLTPKDPAVRPFTPNPLQERILLYVQVIFLPAMTVLCGLLVWRKRRR
                     L"
     gene            15862..17232
                     /locus_tag="PK589_15520"
     CDS             15862..17232
                     /locus_tag="PK589_15520"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNJ21068.1"
                     /translation="MARYWPTVLLAGVLAGLGLYLYFIELPEQRAELVTATQAKQILP
                     FEQAQISALRVRSHSGEVVLSQTPGHPWGITAPIQTDADQRQVQALLRALVTGKVSRL
                     VETHPVSLTPFGLDHPSTVVTVTAGEREETLSIGDAGPLSSTLYVLRASDEAVLLTDL
                     APKDFLNRTLLSFRRKELLQFNQQETEQLRLTYPTTEMVLYGIEEKPKKKWKIRYPIE
                     AEADRTEVQALLFRLEDLKALAIVDPGPEREKLAPLLTKPKVKVTVHAAGTDQVVRLF
                     QPDPASGEAYAQTAQDGPIYKISPSVIKELTKDLFALQDKRLLGAETGDIALLSIKTR
                     DEQYTLVHDRDGWMLEDQPNEKLRQDVADLLVSRIVNLPAEERILKQAGPLAPYGLVA
                     PVAEFIATGKNGRVAGRLALGNQSGGLVYAIGQRIPGVFQVRPDLLTQIPSRLALLAS
                     GPATPP"
BASE COUNT         3889 a         5188 c         4502 g         3642 t
ORIGIN      
        1 cgatcgtccc gatgttcacg tggggctttc gtcgctcaaa tttcgccttc gccatgattc
       61 actccttttc tcagaactct gttttcccag aaccctaccc ttatattcag gtcaccctca
      121 ggaacagaca ggcagctgct cagaccagcc tgctatggag cccacgacgc gaatcgaacg
      181 cgtgacctcg tccttaccaa ggacgtgctc tgccaactga gctacgtggg cacttcctgc
      241 acagcctgta tccgctcgtc gcactctctg tccgcacccg tactcgagta cgctgaattt
      301 ggagcgggcg atgggatttg aacccacgac agccagcttg gaaggctgga actctaccac
      361 tgagctacgc ccgcccacgg cgaacctatc aacacatcaa tactttgcag ccaaggcctc
      421 gcacactcgg ccctggatca ccctgcacac agtcatcata tcttcaatcc gcaacgaccc
      481 cacggattga gccggctcag tttggtgggc aggcaagggt tcgaaccttg gaagccataa
      541 ggcagcagat ttacagtctg ccccctttgt ccacttgggt acctgcccgc gagggtccta
      601 tttccaaacg taagcgcgcg acaaatcaat tcaaagaaaa caagcaccag agcgtttcat
      661 tttccggttc agagaatgaa gtaagcccca ccatgcacag ctctccagca aagcgacagg
      721 gctggatcgc tgataaggtg agggtcgatc tggcttcgaa agcagccgat tttatttgcg
      781 acgcgttcta ttgtcaaggg ggtatgttct atgcaggaac aattctacgg cttcgttgat
      841 gccgcacagg ccccgcgcgt actggcaact ggtcgcgaac gttgaagcac ctgagtggct
      901 tgacgaatga cgtccggctg actccccaat acatccttca tgaggcgcag gagttccggt
      961 gaagacaggc gaaactctgg cgccagaaat cgcttgcgcg ccatacgttt ctcctccagg
     1021 tcctcaggtt cgtagtaccc aatcaacgcc gcgtcctgct cggcctgatc gagccgcgcg
     1081 atccagccag aggtatgggc aggtccccgg gaatcacgcc gcggtttttg atcgacttcc
     1141 agctgcgtcc acaccgccca cccgataaac accgcccagg tttcattgag aacttcccac
     1201 gattccgtct cactcagaaa tcgctcttcc ttgagcgccc ttttctgaag caccggtgtg
     1261 atcaggacct gttgatatcg gcaatgctgt tgctcgcgcg caaacgcaat caaggaagca
     1321 tgggcttccg acggtgtagc ttcctgctcg agaaagtcca tatagacatg gaacagctcg
     1381 tgatacagcg tttccagctc cttatgcgtc aatcgcccca acggtcgcaa cgtgcggccc
     1441 gccgcattca gcgacagcga tcgatccagc accatgcgat ggtccgctgg atgatactcc
     1501 gcggcaaagg catgcagatc ctcgaattcg aatgtcacaa aatgcttcgg cacttgctca
     1561 agaaattgag tgggtagccg gagggctttc gcttccgcca ccaagccgct ccagaggcga
     1621 gaggcatcag gctgggccgc cggagtctca gccagaatcg gcgcagaaca tagcagcacc
     1681 agcagcatgc ctcccaccat cactgcagcg acaccttgca tagaatcgat ataggaccgc
     1741 agacaccgca tgatggcctc gaattgggac atgaacatca cgggaggatt ttcggaatta
     1801 ggcaaggccc ctagacgtaa cggtcccgat catgacccca atcatggccg tgactttcct
     1861 caaccaacgc cagcgattcg aataagcgag gagacacatg atccaaaaac cgggaaggct
     1921 tggacaacaa catgcccgag ctcctgtcat agacattgat cnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn naaaccggga aggcttggac aacaacatgc ccgagctcct
     2101 gtcatagaca ttgatcggat aggtcagaaa cagatagcgc ttcgcccgcg tgaccgcgac
     2161 atagagcaat cgccgctctt cttccaattc ttcatcggta ttgaacgaaa acaccgacgg
     2221 aaacttcccg tccacgaccc acaacagaaa cacgcattgc cactccaggc ctttcgcgga
     2281 atgaatcgtg gacagcacca cctgctcgtc gtccgaacca gccgcttcca ctccgaccgc
     2341 actgccatcc ggcggagcca gagccaagtc cgccagaaat tccgttaggc cggggtaact
     2401 ttcggcaatc gtatgcaaat gatcgaggtc tcgaatccgc ttgggatagt cgtcatggtg
     2461 gtctttgaga atcggcaggt aatactcata cacccgattg acctgctcgg tcgggctcaa
     2521 atcgtcgctc ttcgaaagat tctcgagcac caccgcgagc tctttcaacc ctttccctga
     2581 ccgcccgcta ctgtcccgca acacctgata gggatcagtc accctcacca tggctgcaac
     2641 gagatcctgc gcttttttcg gaccgacccc ttcgaccagc atgagcacac gatgccagct
     2701 caccgcgtca tgcggattca ccaccacgcg taaatgggcc aacaaatcct tgacatgcgc
     2761 agcctcgatg aacttgatgc cgcctcgctt cacaaagggc aatccacagc gtgacaactc
     2821 aatttctaaa tcaaaggagt gaaaactgga acggaataac accgcgattt catttaacgg
     2881 gacaccctcc tcccgcaact ctagaatctt ttgcgcgacg aagcgagact gagcattttc
     2941 cccggcagcc tcgaccagcg ccggcaacgg tccatccagc ttcctcgtga acagatgttt
     3001 cgtatatttc tcaggcgcct cctgaataat ctcattcgcc agactgagaa tcggctgcgt
     3061 gctgcgatag ttttcttcaa gcttataaat ggtcgcgccg ggaaaccagg agggaaattc
     3121 catgatgttg cggaacgtcg cgccgcgaaa ggcgtagatc gattgagaat cgtcccccac
     3181 caccatgacg ttgtcatgcg tggccgccag cttccggatg agttccgcct gcagccgatt
     3241 cgtatcctga tactcatcca ccaagatgta acggaattgc cgtgaaatag cctgccggct
     3301 tgcctcatcc tgcgtcatca ggtcgcgcaa taagacgagc aggtcatcat agtcgagcag
     3361 ttgacgctgt cgcttggcca gctgataggc gcgttgcagt ttgccgagtg catcaaggtg
     3421 atcggcaaaa tgcgaaaact catcaagaac aatttcttcg agcccgcgca aggtgttttc
     3481 acacttactg taaatttccg cgatagtgcc cttgcggggg aaacgcttat ccttctcatt
     3541 gagtccgagc tgggcgcgta acagagcaat cagatcctcg gcatccccgc gatccataat
     3601 ggtaaaactt ggctcaatcc ccagcactcg accgtagcgc cgcaagagca tgttcgccac
     3661 cgagtgaaac gtaccgccgc acacccgttg gctgcgcgag ccgatcaacg cgccaacacg
     3721 ctccagcatt tcttctgcgg atttgcgggt gaaggtcagc aacagaatct gggacggatc
     3781 gacaccggaa tcgatcaaat aggcgacgcg atgcacgagg gtgcgcgtct ttccactccc
     3841 cgctccggca atgacgagtg ccgggccatc cgcggccgtc accgccgcat actgctgggg
     3901 attgagctct ttggcataat ccaacgagaa cttccggacc acgtcctgat ccggagtccg
     3961 tttgagaatg tagggtttcg tgtctcgatc catgttggca tccgattggg cggtgaggct
     4021 catgacggga gcggtcgcgc tgtataacat accaccccgt gccttgcaac gacagcgcgc
     4081 gagccaccgt ccatgatcag ggcgcacgag atcaacactt ggagcttcaa ggtgagttgt
     4141 atataatgcc cgaccagcag ggaaccatta tgacgactaa atcacattcg ttcgctcaac
     4201 agatgcgcac cgtggcggaa ctgatgcttg ccgtgtctgg ggaatccgtc tcggtgatca
     4261 aacgggcggc gccggaacag gccctcaaac tgaagcgcca ggacgaatgg ggcatttatc
     4321 tcgaattttt acgggcaatg ttcaatctga ccgatcgggt atcggcgctc catatcccac
     4381 tgagggaaca accgcagttc atggacgaac tgacggatac agtgatcgat cagctcaaga
     4441 aagtattgga accggcattc ggagccggcg atgaccagat ggaaatcgtc atgacgatcg
     4501 gggccgctgt atccgagagt cgccagacat acgagcggta tcgattcctg gttacagagg
     4561 agagcccggc caaaaatgat ttgtatcgcg atttcagcga tcgtgtcgcc cgtgcggtcg
     4621 gggcatcggg aaacccgaag gttgaggccg cggcgacact ctgcattgcc gctgtcattc
     4681 cggctctgat cggcatcttt gaaggcaaga cgccagcagt cccggccgac gccacaggca
     4741 atgcgaaccc cagtgccgcc agcagcaacc ctcggggcgc caccggggcc gatattaagc
     4801 tggtgagcgt catgtcgagc atcaagggcg aagaagttga gacgcgttgg ggactccacc
     4861 cgcgttttcg tcaagacttg acacaggagg aggcgaaaca actgacggcc accatgaatc
     4921 gagtcgccaa gattctcgga gagcgctacg ccaccgtcgc cttttctgac caatgggcct
     4981 cctggcataa agccggacac gcctgacaca gcattccgga agcgggacca taccggcccc
     5041 ttcctcaccc atcgatcacc ttccacggag gcgtcgtgca agcacctgac cacatacagc
     5101 cccctcagga tatggttccg caaagcctgc atcgacttgg cgaactggcc cgcaacttgt
     5161 ggtggagctg gaaccaaccg gcacggcagc tctttgagtc gatcgaccca accttgtggt
     5221 tcctgaccca ccacaatccc gttcaattac tcgctggagt gaaaccggaa cgattcgcga
     5281 cgctggccag tgatccgacc ttcgcacgac agtactccgc cgtcctgcgc agctttgacg
     5341 aatacatgag cagcaagaac acgtgggcgg ccacggaatt ccctgcactc cagaattctc
     5401 cgatcgccta cttttcagcg gagttcggac tgcacatttc aattccgatt tacagcggcg
     5461 gcctcggcat cttagccgga gatcattgca aggaggccag cgacttaggc attccgctcg
     5521 tagggatcgg gttcatgtat ccgcaggggt atttcaaaca acgaatcaat cctgagggtt
     5581 ggcaggaagc gacctatgtg cccttcaata gatatgattc gccgattcac caagcgctga
     5641 ctccggccgg cgttccttgc accatcaaag ttcaaatcgg ccatcgtcaa gtcaccgtgc
     5701 ttgtctggca ggtccgaaat ggtcggatgt ccctctacct gattgatacc gatgtgcctg
     5761 aaaacacgcc tgaagatcgc gcgctttcgg cgagactcta tggcggcgat caggagacga
     5821 gactgtgtca ggaatttctt cttggcatcg gcggcgtccg cgtcctgcga gccctcggca
     5881 tcaccccagc cgtctggcat tgtaatgagg gccattccgc cttcctcacg ctggaacgga
     5941 ttcgagagtt cgtcagcacg ggacacagtc atgccgaagc gagtgagctc gttcggcaaa
     6001 gcaccgtgtt caccacgcat acccctgtcc ctgccggaca tgacatcttc cccttccatc
     6061 tgatggaccg gtactttcac aattactggg gccagctagg actatcgaga gaagaatttt
     6121 tgcggcttgg agaaacgccg gaaagcgctg gtcacggctt taatatgact gcgctcgcca
     6181 tgcggctatc agcccacgta aatggcgtga gccgggaaca cggccgtgtc tctcgccaaa
     6241 tgtggcaaca tctgtggccc ggtctggcgc aggatcttgt tcccatccgc agcctcacga
     6301 acggcattca cgcgccgaca tggatttctc cggaatcgaa ctcgctctat gccaaatatc
     6361 tcagccccga ctgggcggag cgcatcgacg acccgaccat ctggcaacgg gttacagacc
     6421 tgcctgatga cgccctctgg gcggttcggc aaaccaccag acgcaaactc atgcgattca
     6481 ttcgtgagcg cgcgcgggac ggatggattc gcggacatct ccagcccatg caagccatcg
     6541 ccagaggaac cctgttggac ccagaggctc tcaccattgg gttcgccaga cggtttgcca
     6601 cttacaagcg ggcaaccctc ctgtttcgtg acctggaacg tctcaaacaa ttgctccata
     6661 accgctggcg gccagtccaa atcgtcttcg cagggaaagc ccatccggcc gacgaaccgg
     6721 gccgttactt tattcacgaa gtcctgaact tctgtaacga tcacaagctc ggcgggcata
     6781 tcgcgtttct cgaagactac gacatgcata tggccaagta tttggtccaa ggggtcgatg
     6841 tctggctgaa tacgcctcgt gcgccgttgg aagcgagtgg cacgagcggt caaaaagcgg
     6901 cgctgaacgg agtgatcaac ctcagcgtgt tagacgggtg gtggcaggag ggctacaacg
     6961 gcgccaatgg atggggcatt caacctctcc ctgaaggctc ggacacccaa gcccaagatg
     7021 cgcatgatgc cgaacaactc tttcgcctgc tagaacaaga ggtcgtgccg ctgttttatc
     7081 agcgggatct cgatggaatc ccgcgcggat ggcttcacat tgtcaaagag agcatcaaga
     7141 cggttgctcc gcgcttttgc accaaacgaa tggtcaaaga atacatgcgg cagctgtatg
     7201 cgccggcgac tgcccgtacg ccgaacaaat ggtagcagga attaggtctt ctcccatgcc
     7261 aggccgctac atctccacct gggcatttcc cgcaggactc ctgttaatgg tctggataag
     7321 tcccctgccg gtaggggcag cgacctccac gcctaaaacc tcgccgtcgc cactggaacg
     7381 ggatgccatg acgttctatc gggcggcgga gtacagccgt gtcatcgatc ttattcagca
     7441 ccctccaacc ggccaagaac caagcctgga agccattcgt tacgccatcc tcagctacgt
     7501 gaagatggga aaacctgaag aggcatggaa actttacccc aagcttaccg ctggggatcg
     7561 ccctgatgac ccggcgttat tacgggaaat cgcacgagca tttatcgtgt cccgagtccg
     7621 tgactcccaa gaacatatcc gtatcgcggc ctatacggca ctcgcagaaa tgggggaagc
     7681 cgataccctc ccccttctcg aagacggact gttggattct tcggcacttg tccgggccag
     7741 ggctgcggaa gccattgggc ggagtggact ggcaggacgt tcggccgcgc tcaaacgcgc
     7801 gcttcgagat gaagcgcccg gcgtgcgtat tgcggcaatc aatgctctca gcgatgccaa
     7861 ggtgtcaggt attaccgatc aactgaccga aattgcccgc gtcgatgagg gaccagaatc
     7921 gatcttcgcg tttgcaggcc tgtacaaact tggccgcaca gatgtgctga cggatatttc
     7981 gagtgcggtg actcttcctg accccgaagt acgtatggcg gccttggggg tattggggcg
     8041 cttgaagcgc cctgccagcc tctcgatcct cagtcaggcc gtgtatgatc ctcatcctgc
     8101 cgtgcgtgcc tttgccgcag gcgccttggg agaattcggg agtatcgaag gcatcgcgcc
     8161 actgacgcac gccctgggag atgaagatcc gcgcgtccgc agcgttgcgg cctccagcct
     8221 cggtcgccta ggagtttcag aatcacgaag cgtcgttcag ccgctgttac gagactcaga
     8281 cgaacatgtg cgaattagcg cagtggaatc actgctccgc ttaggcgata ccggtgtcat
     8341 tttgaacgcg gcagccctcg cgcggcatcc cgatccatcc atacgcggcg ataccgccca
     8401 cgcgctcgcc ctcgcgacca ccgccaatgc gctcccgatt ctggaaaacc tgctacgaga
     8461 ccagcaaccg cttcctcggc tcatggcagc ccgcgcactg ggaaagtctc cgctaaaatc
     8521 gctcattcct gccgctaaaa ttggcttgca agattctgat gcggccgtac gtattacttc
     8581 cgccggaagt ctccttcgca tactctctcg aaaagacaaa gtcagcaccc gtaagggctg
     8641 agccgacatg ctcagccctc atcagctggg ttttttcgct caacttcgaa agtccgcaaa
     8701 aaccggccaa ccccgaggct cgacaaattg gctcaggttt tccaataaat aaacctgaca
     8761 acagattgaa tattttgtgc gatcccaaca gcatcaacgc ggcggggcgc cgcgggaatc
     8821 gcacaggcaa acgctgcaac tagaatccgg cagaagggat acctctactg gcttctgtga
     8881 tgcgagggta accggcagtt ttttagcggg acaatctagg tactggcagg aagtcaggac
     8941 attccatcct gatctctccc cagggtagcg cctggtagcc cataagacga acgggcgaga
     9001 agaaggttct cggttactct accgtcaagg ctttctatga cacaacggct ggctgcttct
     9061 tccatttggc aagaatgcgc tcgacccgca tcttgaccac catatccgta tctttcagca
     9121 agggcttaat cgcttcgcga ccacgttcat ccccgatagc ttcgagcgcg gccgccgccg
     9181 tcagccgtgt aaaccaatct ttgttctgca acatctcgat caacggatcg atggcttccg
     9241 gcgacttaat gcggcccaac gctatcacgg cttgcttccg aactaactcc tctttgtcct
     9301 ttagagcctt gatcagcccg ggcaacgcgg gctcgccaag gtttaccagg gcatcggtcg
     9361 catcttccat gaattcatca ttccgaagct gctgcatgag cggctccaac acccgctcat
     9421 cacgaatctt tcccaatgcc atgatcgcag acttccgcac atcccaatcg cgcaaaagct
     9481 tgagaagtac atcaacggca ggagatccga tttgtccgat tccctcaatg gcgacctctc
     9541 gcacttgcca gtcaccgtca cgaagggctg ccgcgagggg ttcaacgcaa cgttcgtccc
     9601 ccatctcgcc cagcgtaatt gccgcttccc gccgcacaac ccaatcagga tctttgagga
     9661 ggtcgatctg aatatcgatt tcatccttga ccttctcctc ctccagcacg acttcctgca
     9721 ttggctcaat gagctcctgc tcccccacgg aggaatccaa ggatgcggca agttggtcag
     9781 agacttgatc tgtcaaagcc tcgtcttcga ctgggccgac aggggaaaca gcctgtgctt
     9841 gatctttttt ttcgccagta tgttccatat caagaatcct tattccgcag gagggttgga
     9901 gtagggtcag atgcttcgat actgcagttc atcaggattt tgcttcagcc tgtttccctg
     9961 ccgcatgctt tttccttaac acgagtgctc gtcgtttacg ctgcacccgc ttgagtcgtt
    10021 tacgcaagga ccgcacggct ggatcaccag atttgctctc gtctttagtg ctaacggcga
    10081 ttttcttctt cagtcggact tcgtctgatt caccggtcgg tttctttgcc attcagcgtc
    10141 aacctccaac agttaggatc aacatcaacg gaaatagcgg catgctcagc atgccaatct
    10201 agatcgggca gtctaatcaa gtgcaggagg aggtgtcaac tgagggaatg tcgattgctc
    10261 gtatcaggaa acgatgagcc cggggacggt attatgcgcc gaatggtctg ccgctaacat
    10321 actgccgatc cgccgttcac ggcgaacgta caacgcttct tggggcatca ttcggagagg
    10381 tacgacaggc acttctaccc cctcttcctg gcgcgtatgc cgcttcatga ctcgcgtatc
    10441 ggtattcaac aacataccag ctacggaggc tatcagtgaa gagggtatcg gtgccaccgg
    10501 acgatgattg ccgatgactt ctatctgcac agggctagtg ccggcttcca gcatacccag
    10561 ttcacgagca gcggcttgcg aaaggtccag cattcggcct gacacatatg gcccccgatc
    10621 attaattcga acctgaaccg attttccatt ggccaggttg acgactcgca cgagactgcc
    10681 aagaggcaac ttacgatgag cagccgtata ggcggtcatg tcaaagactt ccccgttcgc
    10741 tgcaagtttc ccgtgaaact cctttccata ccaggacgca acgcctcgat ccttgatgcc
    10801 gagatccagg gggagatcgc ctttgggaag ggctgaacag gcactgagga ggaggcaggc
    10861 taccagggga gcaagagcag atcccgtctt acaacgagaa cggttccaga aattcctgac
    10921 catagaagca cctcgtttga aagcctgaaa tcactgtctg ctggaacagc tcctcaaaag
    10981 tgatttcagg ctacgggaga ttccccttgg cgacaatacc gtctgggtag caaggccccc
    11041 tacaaatggg caacgaacct tgcatcactg aacacaagtt gcggtttttc ccgccggaaa
    11101 tacgcgacta aagaccgaac tgccttcaaa tgagccagca ctgccagaac tctaagtctt
    11161 tacacgacag tacagttcac aaatgaacgt ccccccagag atcaaaagga cggaacgtca
    11221 cgatctgtca ggacctaaag cgccccaact ccggattgct ctctaccgaa accgaatgga
    11281 ccgatcttcc agtttcgtgt tcaaaacaaa tcgctcaaaa ttcttttcca gcacttcacc
    11341 gagaagctct tccccgtcct ggggatcaaa ccaaaataca ttgtcatggc taccggaatg
    11401 cgcatcggtt cgaacgatcg aactgccatc ttcccgattg tttgcctgga taaccaactt
    11461 gctgcgggcc tcaatatctg tcatgagaaa tccccgcttc gcatcgaccg tcaggtcgag
    11521 aatttttccg gacaacacga tattgggacc attttctgat tccgctgggg cagccttctg
    11581 atactgcacc agccagcctt tgcgcttgag atagtcggca agtgcttgac cggtttcctc
    11641 accggcactg ccgctcgaga cgtgaaagtc cgtttcaccc ccccacatgg agcttcgact
    11701 tcccaccttg gcatggtcgg cccgcgcgtc ttcaaatgga atgaccacaa ctttcggtcc
    11761 cgccatcggc ttaaccgctg aagaggcgtt ggtcatctcc ttggccttgg gggtcaaatt
    11821 catcggaatg atatcgcctt tcccggagca gcccatgagg agcagcaacc cgacggctac
    11881 cggcgcagac atcccccgca ccacatgctg tcgaatcata aaatctctct acctccttgt
    11941 gaaaaacaca acgtcacgag aatgaatgcg cgtgattaat tgcgatggag tgcgatggat
    12001 cgaaccgtca gaccatcgcg agtcttcaca tacttcaacg tcaccttatc tccagcatgg
    12061 atttgatcga gcgtgatccg ttttttccca cgcataatgg tggcgccttg cttaaccact
    12121 gccccgacca cgatagcttc actcgaccca tgctgcccct tgacgacgat gacagggggg
    12181 tcttcgtggg catgcacgtt ctcaaccgtc cccttgatct catggacggt attactggcg
    12241 gcatacacgg atgtcaggaa acttccaagt ccggcaagag ccacgcacaa cgcggcgccg
    12301 atgacgagta cgccgagcaa actcggcctc gcttggttca tgtcactctt cattcaggag
    12361 agacctcttc gtcaggcatt ccaccaggcc gcccggtggc taaagtcgcg gaactgtagc
    12421 agttacgaat cacgctgtaa agacggactc acgcttttct acgtccttca gcacaacgta
    12481 tccttctctt gactgcatct cacacggtcg gtaatatcag gcgcgatttg ccacaccacg
    12541 tgccgtacca aacgaacagc aggaatcaca ccagccagcc gtcaatcccg actgcatgat
    12601 cgaagtttgt aacattacta aacgctacgg ccacctcacc gcgatcgatc gcgtcacctt
    12661 tcgcgtggaa aaaggagagg tgctggcctt cctcggccct aatggcgccg gcaaaaccac
    12721 gacgatgcgt atccttacct ccttcattcc cgcaacggaa ggaaccgcgc acgtcgcggg
    12781 cttcgattgc gcggaacagc ctgacgaggt aaagaaacga attggatatt tgccggaaac
    12841 tccgcctgtc taccaagaac tcacggtcgc cgagtacttg ggttttgtgg ggaagctgcg
    12901 cggattgagc ggaacaggtc taacgcaggc gctggaacgg gtcacggaac ggctttcttt
    12961 ggggactgtc cggcagcgac ttattgccaa tctgtcacgc ggttatcgtc aacgggttgg
    13021 tctggcccaa gccttgattc acgatcctcc cgtcctcatt ctggacgaac ccacggttgg
    13081 cctcgatccg aagcagatca ttgaaatccg tgaattgatc aagagcctgg ccggctcgca
    13141 ctcggtgatt ctcagcacgc atatcctgcc cgaagccacc gcagtctgtc agcgcgtcgt
    13201 gatcatcaat ggggggcgga tcgtcgccga agacactcct gaccaactct ccgcccgcct
    13261 ccggaagtcg gaaaagatca gcgtcaccct gaaagctccg ccggtcaatc tggcggagcg
    13321 atttcgcgag gtcccaggaa tagaacatgt gctgaccaca gccgacccgc acacggtgct
    13381 catcgaatgc gcgctggggc gggatatccg ggaggacgtg gcgcgctttg ccgtcggcca
    13441 aaactggggg ctattggaaa tgaaacccgt ctcgatgaca ttggaagatg tcttcctgaa
    13501 gctgacccaa cgggaagacg agccgccgaa gtcgaacgac accaccccag gcgagcacca
    13561 ctaagcgatg ccacccgtcc aagcgatcat tagaaaagag ttgcgctcct atttcgtctc
    13621 gccgatcgtg tatgtcgtcg gcggagtctt tctcctgacc ttcggattcc tggcctatct
    13681 ctacattgtc ttcaccggag cccaggccat tcaactcatg caaatgcaag gcggcacggc
    13741 gcagatcaat ctgaatgatc tcgtgttccg caatcttttt gccagcatgc gattcgtgct
    13801 gctgctgatc cttcccatcc tcaccatgcg actgctggcc gaagaacgca aacttcgaac
    13861 gtttgaattc ctgatgaccg cccccatccg tgtcagcgaa atcatcgtcg ggaaatttat
    13921 cagcgtctat ctcgtattta tggggatgtt gctgttgacg accttggttc cgctcacgct
    13981 ggcactcttc agcgattttg attggtaccc cgtattcacc ggctacttgg gcttgttact
    14041 gctcggcggg ttttttctcg ctgttggcct ttttgcctcg tctgtcaccg aaaaccagat
    14101 tgtggccgca ttcaccagct tcgggctgct gctgatgtgc tggctgctgg ccggactggg
    14161 ctcgctcctg ggcgacacgc ccgtaggaca agtcgtctcc tatctttcct tcatggagca
    14221 ctatgaccac ctggtgcgcg gactgatcga cacgaaagac ctgatgtatt acgtcagcgg
    14281 aaccgtactg atgttgttcc tcgctcaccg gattgtggat gcctcgcgat ggaaatgaca
    14341 cgcaatcccc ttcctatcgg tgccatcgga acagggctgg ccattgccgg agtgattgcc
    14401 tactctctag ttccggacaa gctctggctc gtcaccctcc tggagggact ggccttggcc
    14461 tgttttattt gggcgtttgc tacacatttt gaccaggtca aacgcttctc ctctcaacgg
    14521 tccacgcgaa tgggcgcgaa cagtgcgatg atggttgccc tctttctggc catcttagcc
    14581 atcttgaatt tcctcgccgc cagacattcc atccgctggg acttttcaga gaatcagaac
    14641 tatacgctcg caccggaaac ctatcgcgtt ctccgcaatc tcacccgaga cgtcaacgtg
    14701 acggtgttca cgcgggaaaa agatcctggg taccagggct acaaggaacg cttcgacagc
    14761 tatcggcaag ccagcccgaa gctgaccgtt cagttcatcg atccggaacg ccagccgaag
    14821 gtcgcacaga attacggcgt cacccgcacc gatgtggcga tctttgaaag tggcccacaa
    14881 tcggttcgca taacgtcgcc agcagaggtg gagatcaccg gcgcactcgt gcgcgtgtcg
    14941 aaggacagca aaaaacgggt agtgtttcta gaaggccacg gggaacgcag cttggaagac
    15001 aaggaccgtg gcggaatggc actcaccaag gaagtgctgg aaaagcaggg gtacgagatt
    15061 ggcaccgtga cattgctgca agagactgct gtcccgagca acacagacgt cctcgtgatt
    15121 gccgggcccc gacgcgccat tacgaaggaa gaacaggacc gcattcagac gtatgtcgca
    15181 caaggcggtc acctcctgct tctactcgat ccggacacac aagcaagcat ggataccctc
    15241 ctcaagatct ggggtttgga attaggagcc ggcgtcctcg tggatctgca agaccgtctc
    15301 gcacaaggtg acctgacagc cctgctggtg cgcacattca ccgaccacga aatcacccac
    15361 gaactcaccg cggctgtctt atttcctttg gcccggcatc tgacctttca tgaagagcag
    15421 gcaaaggact gggactacgt tccattggcg cgcacgtcac cgcggagctg ggcagaaaca
    15481 gatatgaagg gtcgggtcgt cagctatgac gaaaaagaag atgtgcaagg gcccttggcg
    15541 ctcgccgctg cgttgacgcc caaacaagcc cccgaggaag ggaagccccg tcccgccgtc
    15601 gtcgtggttg gcaactctgc cttcgccagc aatggcttca tcaacttcgt cggaaacagc
    15661 gacttctttc aacgcacggt gggatggctc tccgaagagc gtgacttgat ttcgctgaca
    15721 ccaaaggacc cggcggttcg accatttaca ccaaatcctc tccaggagcg gatcctgctc
    15781 tacgtgcagg tgatttttct gccggccatg actgtgctct gcggtctgct ggtctggcgg
    15841 aaacggcgtc gcttgtaggt catggcgcgc tattggccga cagtactcct ggcgggcgtc
    15901 cttgcagggc ttggcctgta cctttacttt atcgaactcc ctgagcagcg cgctgaactt
    15961 gtcaccgcga cccaggccaa acaaatcctg ccgtttgagc aagctcagat cagcgcctta
    16021 cgggtccgca gccactcggg ggaagtggtc ctcagtcaga cgcctggcca tccctggggc
    16081 attacggcac cgattcaaac cgacgccgat cagcgtcagg ttcaggcact gctgcgggcg
    16141 ctcgtcacag gaaaagtctc gcgtctggtt gaaactcacc cggtctcatt gacgccgttc
    16201 ggtctggacc acccctccac cgtcgtcacc gtgacagcag gcgagcgcga ggaaacactc
    16261 tcgatcgggg atgccggccc gctctcgtcc accctctatg tattgcgagc gtcagatgag
    16321 gctgtccttc tgaccgacct ggcgccgaaa gactttctga accggaccct gctctccttc
    16381 cggcgcaaag agttgctgca attcaatcaa caggagactg agcaactccg tctcacctac
    16441 ccgaccacgg aaatggtgct gtacgggatt gaggagaaac cgaagaaaaa atggaagatc
    16501 cgttacccca ttgaggctga agcagatcgc acggaagtcc aagcgcttct atttcgcctg
    16561 gaagatctca aagccttggc catcgtggat ccggggcccg aacgggaaaa gctcgccccg
    16621 ttactgacca aacccaaggt gaaagtcacc gtgcatgcgg ccggaaccga ccaggtcgtt
    16681 cgcctctttc agcccgaccc ggctagcggc gaagcctacg cgcaaaccgc tcaggatggc
    16741 cctatctata aaatcagtcc ctccgtcatt aaagagttga ccaaagacct gtttgccctg
    16801 caggacaaac ggctgctcgg agccgaaacg ggcgacatcg ccctgctctc cattaaaacg
    16861 cgagacgaac aatacacgct ggttcatgac cgagacggat ggatgctgga agatcagccg
    16921 aatgaaaaac tgcgccaaga cgtggccgat ctgctcgtca gccgtattgt gaatctcccc
    16981 gcggaagaac gtattctcaa gcaagcgggc ccattggcac cctatggact cgttgccccc
    17041 gtggccgaat tcatcgcgac cggcaaaaac ggtcgtgtgg ccggacgtct ggccctgggc
    17101 aatcaatcag gaggcctcgt gtatgccatt ggccagcgga taccaggcgt attccaggtc
    17161 cgtcctgacc tgctgaccca aatcccttcc aggttggccc tgctcgcaag cggcccggca
    17221 acaccgccct aaaccgcgac gagagtgttt ccaggaagag ggggattatg ctactgtgcg
    17281 cccatcgctc ttgatcaagg aggatccttc aatgatgtac t
//
LOCUS       DAOQDS010000077        17056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1369_length_17056_cov_21.000706, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000077 DAOQDS010000000
VERSION     DAOQDS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17056
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1369_length_17056_cov_21.000706"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PK589_15525"
     CDS             complement(<1..756)
                     /locus_tag="PK589_15525"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HNJ21069.1"
                     /translation="MVDRSAYLARIAYEGPVDPSIETLRRLHRAHVLTVPFENLDIHL
                     GRPISLEPLDLFRKIVTGKRGGYCFELNGLFALLLEDLGFAVTRLAARVLYGAEGVRP
                     RSHELLLVQLGETASVADVGFGGNGLLEPLRLMDGYEERQGADRFRLLAQEQGGYRLQ
                     WEMESVWTDLYSFALDPWLPIDFAFANYYHSHSPDSLFMQRRICTMPTLKGRKTLIDN
                     LLKIRSSNGTQELHVKSEGERQALLQQHFGVTIN"
     gene            908..>1248
                     /locus_tag="PK589_15530"
     CDS             908..>1248
                     /locus_tag="PK589_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNJ21070.1"
                     /translation="MASRTPPTRSTDELIIEGARQNNLKNISLRIPHNKVTAITGLSG
                     SGKSSLAFDTLFAEGQWRYVESLSTYARMFLDKVNRPDVDRITNIRPAIAIEQKNPIR
                     TARSTVGTATEV"
     assembly_gap    1249..1348
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1354..>3512
                     /gene="uvrA"
                     /locus_tag="PK589_15535"
     CDS             1354..>3512
                     /gene="uvrA"
                     /locus_tag="PK589_15535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNJ21071.1"
                     /translation="MPEHRESGLQVVLDRLVLRPDNRHRLIEAIEVAFQEADGVCQVD
                     VIGHGLRTYSTHFRCQGCGRTFEPLRPLLFSFNHPLGACPECKGFGNILRYDRDLVIP
                     DRSKSLAGGVIEPWSKPGSDWWQKQLLLAMKKLEVDLTAPFQELPSEVQQLIWEGSDQ
                     VEGVRQYFDYLETKRYKLHVRVLLSRYRSPATCPICQGSRLKPAARFVKLAGHDFIQL
                     NDFTIQAATEWFQRLALPAFDAEIAKDILRQLQAKLNFLLRVGLNYLTLSRQTKTLSG
                     GEAQRIALANQLGSRLVGTLYVLDEPTIGLHARDTDTLAGILRDLATEGNTVVVVEHD
                     PLMIQAADHIVEMGPASGEQGGQVVCAAPRAPFIRDPKSLTARYLRGEERIPLPKTRR
                     SGNGKVLSIAGAAEHNLKNLLVRIPLHMLVCITGVSGSGKSTLIEDTVYRAAARAFRI
                     ESLPMGRFQAIKGLEYLKGVRLIDQQPIGRTPRSNPITYLKAFDEIRQLFASERDALR
                     QGLTPGHFSFNAAGGRCERCEGNGYEKLEMYFFEDIYATCEQCNGRRFKPEILAIKYR
                     GKTIHDVLNLTVTEAQAFFSGSPKLTEKLYLLSSIGLGYLRLGQAANTLSGGEAQRLK
                     IAAELKDPSAHNQLYILDEPTTGLHLDDIKKLLAVLHKLVDAGNTLIVVEHNLDVIKT
                     ADWIIDLGPEGGEAGGQIVAEGRPEQVAKVAQSHTGR"
     assembly_gap    3513..3612
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3806..4366)
                     /locus_tag="PK589_15540"
     CDS             complement(3806..4366)
                     /locus_tag="PK589_15540"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNJ21072.1"
                     /translation="MTTPLTKGVLLVATPALNDPNFRQAVVLLCEHGPEGALGVIVNR
                     PTAMSISEALPQVPILEGQPHVLYSGGPVQTNQVMMLYRISDTPENSHQVFDGVCLGG
                     DLEIMERILMEQPGKESFRAYLGYSGWGPGQLESEMQTGSWITLPADPSIVFEKEAAR
                     IWSDIVLSLDETSRHYADMPFDPSSN"
     gene            complement(4431..5225)
                     /locus_tag="PK589_15545"
     CDS             complement(4431..5225)
                     /locus_tag="PK589_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNJ21073.1"
                     /translation="MMRVGYLQGDPVFGEVAHNLDTVTAQLEHAEADLLVLPELFATG
                     YQFTSQEEVSRLAEAVPDGPTTQRLLEIAARRKTTIVAGLAERAGTKYFNSAVVVGPK
                     GFIGCYRKTHLFFEETLFFNPGDSGFQVWDIGAAKIGVMICFDWYYPESARTLALLAA
                     EIIAHPSNLVLPNCPDSMVTRCLENRVFSVTANRIGSEARGGKDRLTFIGLSEIVSPR
                     GQILHRAPRDREELTIIEIDPAEARRKALNSYNDLLRDRRPAMYGV"
     gene            complement(5307..5663)
                     /gene="smbP"
                     /locus_tag="PK589_15550"
     CDS             complement(5307..5663)
                     /gene="smbP"
                     /locus_tag="PK589_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HNJ21074.1"
                     /translation="MQTAMWRGVVVAGVMMALVGAPMVSSVAFAAGNKHQAEAVEHAK
                     EAVAHGKQGHADALVKHAEAALKHAEGAMAETKNPHVGEAIKGLKDGIEHGKAGHADV
                     ATKAVENALPHLSEGM"
     gene            complement(5744..6637)
                     /locus_tag="PK589_15555"
     CDS             complement(5744..6637)
                     /locus_tag="PK589_15555"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNJ21075.1"
                     /translation="MQHPLLIDCETLQTRLGQPGLVILDVRGRSTYEFGGHIPGAVHS
                     TWHEYSDPEAIAKGLLDPDVKRIEKRVQALGINRDNEVVIYSNPFDNWGDEGRMFWML
                     EYLGHKNIKVLDGGWVKWIHERRPFEHGAVRPTPGNFTVSPVASAIIMKDELKGIVRK
                     PHPTTTIVDARSLEEFLGKEVSGIPRPGHIPGAVHVAWSGFLNADATVKDLDAIHAQL
                     ELKGLNPAHETVCYCTGGVRSGWLYFVLRLAGFERLRNYPGSWWEWSRDFACPVEKDL
                     LALQKLLGLSESQVPTGMRPS"
     gene            complement(7372..8646)
                     /locus_tag="PK589_15560"
     CDS             complement(7372..8646)
                     /locus_tag="PK589_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21076.1"
                     /translation="MKWWLKLLLGLAVISGLVYLYYTEVKPVVIFGLRSEYAHAIPFQ
                     KIPEGLTSLKAESCGQCHREIYEEWKTSIHAHAYEDPFFQAYWKKDKQVWVCLNCHTP
                     LENQQPTLVKDIPRGRVEKATQEPNPHFDAALQKESITCAACHVRDGVILGPFDDSAA
                     PHPTKFDPTFRTAQFCSRCHNVVSGPAQFYNVGPCGTYAEYEGKYFMQERGFICQSCH
                     MPEVDRPVAENGPIRRGRRHLWRGGHDPDMVKRAAAIQVKVDPPKPKPGDQVTIALTL
                     TNAGAGHKLPTGDPDRHFTVEFTVKDSNQQILAEKSHTMGRWIMWQPAIVELYDNRLL
                     PLASREYQFAYRMPEASKGVTLKARVRYHILTDGQHEMLKKKYGLMADDPYAFTVYER
                     EVPLNEQLAAAFADPLPEPPPMACANPSAPQG"
     gene            complement(8643..9587)
                     /locus_tag="PK589_15565"
     CDS             complement(8643..9587)
                     /locus_tag="PK589_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNJ21077.1"
                     /translation="MGRTGLVYDPRYLAHDMGAGHPESPNRLRAIMQRLEQSGLAAEL
                     TRLAPRTAEDEWITLVHRPEYVGHLNREAPTSGRVSLDADTSMSPGSLLAAYLAAGGA
                     LSGVDAIMADQVQHVFCAVRPPGHHAEAGRAMGFCLFNNVAIAARYAQKRYGVQRVLI
                     VDWDVHHGNGTQHSFEADPSVLFFSTHQYPHYPGTGRATECGTGAGEGFTINVPMEAG
                     EGDDDYRAVFQKVLVPAADAFKPELVIISAGFDAHRDDPLASMGLTEEGYADLTSIVA
                     GIATRHCRGRLLSSLEGGYNLTALAASVERHIRALVAS"
     gene            9717..10037
                     /locus_tag="PK589_15570"
     CDS             9717..10037
                     /locus_tag="PK589_15570"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ21078.1"
                     /translation="MPNPRIEPLKRVLAMEPDDDVAWFGLGKAYMEDANFEEAAKALR
                     QCITVKPTYSAAYYALAQSLLKLNRVEECRQVSDQGIDVSTKNGDAMVTKNLELLKSS
                     LPAA"
     gene            complement(10109..10438)
                     /locus_tag="PK589_15575"
     CDS             complement(10109..10438)
                     /locus_tag="PK589_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21079.1"
                     /translation="MTEQEINRAIQYVTAATSYGRDTVAEILRTGLSELSQLSTQSTR
                     RFEREDLMGYLCQWTMKRTGHPEPLVREVLDGAGRWLDEVATTLAEQREAEANAGDDD
                     ETGAASA"
     gene            10593..12413
                     /locus_tag="PK589_15580"
     CDS             10593..12413
                     /locus_tag="PK589_15580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNJ21080.1"
                     /translation="MAVSRKTASATVSSRRSTTSREFADRWDLADLVADPVKQFDRYL
                     KDLTAKVTRFEAARPDLSATMSEQSFLNLLTLSEQIARDSGRLGAYSYLWFSEDTKQL
                     QARSFKTKVEEHLTSLQNRLLFFDLWWQSVDEKNAERLMANSGDFRYHLETIRRFKPH
                     TLSEPEEKIINIKNITGQSAVHQLYDVVTNGFTFTLRIGGKNKTMNREALTAYLRSPK
                     AGMREAAYREMYRVYESQQDLLGEIYKTLINDWKAENLQLRHFKTPLASRNLSNDIPD
                     SAVEALLAICMKNAPIFQRYFKLKAKLCKISTMNRYHIYAPHRAEQKSYRYPDAVRMV
                     LDAYYGFSPRLAELAQRVFADRHIDARMKAGKMGGAYCYSVTPNVTPYVMLNFTGEAR
                     DIATMAHELGHAVHAMMAEQHNVFTFHSTLPLAETASVFGERILSDALMASESSKSVK
                     QSLLVNQLDDIYATVMRQAYFVAFERTAHDMVAQGATTNDLAGTYMTLLKQQFGKSLR
                     VPQEFQWEWLTIPHLYASPFYCYAYSFGNLLVLALYRMYQEQGAAFVPKYLDLLAAGG
                     SKAPQAILAQVGVDMNSHAFWQSGFDAISGMVDQLEETMR"
     gene            complement(12498..12743)
                     /locus_tag="PK589_15585"
     CDS             complement(12498..12743)
                     /locus_tag="PK589_15585"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNJ21081.1"
                     /translation="MKKKTAPKKAATRTTKQVAKPAVSEKQPGPVQDIQVRIAARAHE
                     LYEQRGCLDGYHLRDWLEAEREILGHPVEMDKSSERI"
     gene            complement(12755..13030)
                     /locus_tag="PK589_15590"
     CDS             complement(12755..13030)
                     /locus_tag="PK589_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21082.1"
                     /translation="MNDGTDRNDGYDVDSAVWAYLQRCRICSMEELFRHVPTFTANQL
                     FLTVDRLSREGKVVLRYRNRAEYVMVRTGIADEWPHEMSGAPFGDRT"
     gene            complement(13129..13458)
                     /locus_tag="PK589_15595"
     CDS             complement(13129..13458)
                     /locus_tag="PK589_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21083.1"
                     /translation="MKTLLSHACVILAWSTLAGCVATSVREPFPALSDASVMGMTRGP
                     SESRLQWHEDAQELRTFADRHEVEAEVLLQHPVPTDARLIQQRRALARQLRAAADQID
                     KRADEMP"
     gene            complement(13782..14195)
                     /locus_tag="PK589_15600"
     CDS             complement(13782..14195)
                     /locus_tag="PK589_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ21084.1"
                     /translation="MEGYGKRVLIADDEESVRRLVAIVLEQAGYIVHAAVDGVEGLGE
                     MKKRRFDAVISDFHMPRLDGEQFLLLCRLMWPDIPVVLLSAELLELPAALKQQGACIL
                     VSKPFSPQMLLQALHESLSLPESEQASDRITSKAS"
     gene            complement(14266..14499)
                     /locus_tag="PK589_15605"
     CDS             complement(14266..14499)
                     /locus_tag="PK589_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21085.1"
                     /translation="MACSRCQGLMVDETLFNPSEGVSHTWVPVVRCLNCGNLEDALIR
                     LARGISGRLGRRTRPGPQRRGVWSEEIRGRQKE"
     gene            complement(14537..15442)
                     /locus_tag="PK589_15610"
     CDS             complement(14537..15442)
                     /locus_tag="PK589_15610"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNJ21086.1"
                     /translation="MWRYTTIGLIIAGLCGLSGCGNTVSPGQRGLRWYPLTEGLTTET
                     LKSGFYWRAPWNDVYVYDVRLQSYTELVDALSSDDLLVKLKAAIIMRPIAEEVYFLAQ
                     EIGPDFYPRVVRPELLAAVRSVVSNYPMVAVPEMSAEIASKVQAVVVEKLKGRHLEVH
                     SVALADIELAKIVLEAVERKQAKEQEKEQKEFELVIAEKEAEIARRRARGEGDAIRIR
                     SEGEAEGLKIRALGQANAQQTITKTLTPEYLQYKLYDSPNAKMVLLPDQLRVPILINP
                     DQDRGTRSTPESLMRTEQELMGRGR"
     gene            complement(15471..15836)
                     /locus_tag="PK589_15615"
     CDS             complement(15471..15836)
                     /locus_tag="PK589_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21087.1"
                     /translation="MMRVSCPAVVRCLVLVAACQMPACGNTASVFDLETNDPKQDQEK
                     IAEFYAHEATRLRQMAQDYDHRVAVYERLFGPHSDWVEGTRLLSQSYEAAAQEQERIA
                     ARHQRLHDRRSHAADQESR"
     gene            complement(15833..16102)
                     /locus_tag="PK589_15620"
     CDS             complement(15833..16102)
                     /locus_tag="PK589_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21088.1"
                     /translation="MIRQSGFPSDDVIDNQILVLLHRHRRLTFLTLADALPLYTWQSL
                     FAALNRLRTQEQVELLPLAADYEVIWRSGRETEPLLSKGGHLQVR"
     gene            16427..16786
                     /locus_tag="PK589_15625"
     CDS             16427..16786
                     /locus_tag="PK589_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNJ21089.1"
                     /translation="MVHIKRIYSEPDPQDGIRILVDRIWPRGCTKERARLNDWQKELA
                     PSTALRTWFGHDPRKWKEFRSRYLAELAQPAQRRAIEELVALARSQTLTLLFGSSDVK
                     HNQAVVLKELIDRAERE"
BASE COUNT         3649 a         5158 c         4625 g         3424 t
ORIGIN      
        1 gttaatggtc actccgaagt gctgctggag cagcgcctgg cgctccccct cactcttcac
       61 atgaagttct tgcgtcccat tcgagcttcg aatcttgagc aggttgtcga ttagcgtctt
      121 cctccccttg agggttggca tcgtgcaaat ccggcgctgc atgaagaggg agtccggcga
      181 atgggagtgg taatagttgg caaaggcaaa gtcgatgggc agccagggat ccagggcgaa
      241 ggaatagaga tctgtccaga cactctccat ctcccattgc agccgatagc cgccttgctc
      301 ctgcgcgagg aggcggaatc gatcggcgcc ctgccgctcc tcatacccat ccatcaacct
      361 caaaggttca agtaatccat tacctccaaa gccaacgtcc gcgaccgatg cagtctcccc
      421 aagctgcacc aacaacagct catgactgcg cggccttacc ccttctgcac cgtagagcac
      481 ccgggcagcc agcctggtca ccgcaaatcc aaggtcttcc aatagcagcg caaacaggcc
      541 attgagttcg aagcagtagc cgccccgttt accagtaacg attttccgaa agagatccaa
      601 cggctccagc gagatcggcc gcccaaggtg aatatcgaga ttttcgaacg gcacggtcag
      661 cacgtgagca cgatgcaacc tacggagagt ctcgatggat ggatccaccg gcccttcgta
      721 tgcgatgcgc gcaagataag cagaacgatc caccatacca tcctctcagt cgtccatatg
      781 atggcgccat tatacgagtc catccaggcc gatcgcacga gtctcatcgc tggcaggtcg
      841 atgacattgc agacgacggc agcgcttgct acgatggtcg gtcatccatg agaaggagcg
      901 cttcgccgtg gcatcccgta cacccccgac tcgatcaacc gacgaactga tcatcgaagg
      961 ggcacgtcag aacaatctca aaaacatttc attgcgcatc cctcacaaca aggttacggc
     1021 gattaccggc ctgtcagggt ccggcaaatc ctccctggcg ttcgatacgt tgtttgcgga
     1081 aggacagtgg cgctatgtcg aatccctctc gacctatgcg cgcatgtttc tcgacaaagt
     1141 gaatcgcccc gacgtcgacc gcatcaccaa cattcgcccc gccattgcca tcgaacaaaa
     1201 gaatccgatt cgcacggcgc gctcgaccgt cggcacggcg accgaagtnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnntc acactgccgg aacaccgcga gtccggcctt
     1381 caggtcgtgc tcgatcgcct cgtactgaga cctgacaatc gccatcgctt gatcgaagcc
     1441 atcgaagtcg cctttcagga ggctgacggg gtctgccagg tggatgtcat cggccatggg
     1501 ctccggacct atagtaccca ctttcggtgt cagggttgcg gccggacctt cgagccgttg
     1561 cgccccctgc tcttttcatt caaccatcca ttaggcgcct gccctgagtg caagggcttc
     1621 ggcaatatcc tgcgctacga ccgggacctg gtcattcccg atcgcagtaa gtcgctcgcc
     1681 ggcggcgtga tcgaaccctg gagcaaaccg ggatcggact ggtggcagaa acagcttttg
     1741 ctcgccatga agaaactgga ggtcgatttg acggctccgt ttcaggaact gccgagcgag
     1801 gtgcagcagc tgatttggga aggcagcgac caggtggaag gcgtccggca atacttcgac
     1861 tatctggaaa cgaagcgcta caagctgcac gtgcgagtcc tgctcagtcg ctaccgcagc
     1921 ccggcaacct gcccgatctg tcagggcagc cggctgaagc cggccgcgcg cttcgtcaaa
     1981 ttggccgggc acgatttcat tcaattgaac gatttcacca ttcaggctgc cacggaatgg
     2041 tttcagcggt tggcattacc ggcattcgat gcggaaatcg ccaaggatat cctccggcaa
     2101 ttgcaggcca agctgaattt tctgctgcga gtggggttga actaccttac gctctcccga
     2161 caaacaaaaa cgctgtccgg aggggaagcc caacggatag ccctggccaa ccagctgggc
     2221 tcccggttgg tgggaaccct atacgtactg gacgaaccga ccatcggact gcatgcgcga
     2281 gacacagaca ccctggccgg catcctccgc gacctggcga ctgaagggaa caccgtcgtt
     2341 gtcgtcgagc acgacccgct gatgattcag gctgccgacc atatcgttga aatgggcccg
     2401 gcttcgggag aacaaggcgg ccaagtggtc tgcgcggcgc cgcgtgcacc attcatccgc
     2461 gacccgaaat ccctgactgc ccgttacctg cgaggtgagg aacgaatccc gcttccgaaa
     2521 acccgccgct ccgggaacgg caaggtgctg agcattgccg gagccgccga acacaatctc
     2581 aaaaatctac tagtacgcat tcccctgcat atgctggtct gcatcaccgg cgtgtccggc
     2641 tcgggaaaaa gcacactcat cgaggacacg gtctaccggg ccgccgcgcg ggcctttcgg
     2701 atcgaatcac tcccgatggg gaggtttcaa gccatcaagg gactcgaata tctcaaaggc
     2761 gtccgcctca tcgaccagca accgatcggt cgtactccac gctcgaaccc gattacctac
     2821 ctcaaggcct tcgacgagat tcgccagctc tttgcctccg aacgggacgc gctgcgacaa
     2881 ggcctcacgc cgggacattt ctcgtttaat gcggccggcg gccggtgcga acgttgcgaa
     2941 ggaaacgggt atgaaaagct ggagatgtat ttcttcgaag acatctatgc aacgtgcgaa
     3001 cagtgcaatg gccggcgatt caaaccggag attctcgcta tcaagtatcg cgggaaaacc
     3061 attcacgacg tgttaaatct gacggtgact gaagcgcagg cgtttttttc cgggtcgccg
     3121 aagctgacag aaaagcttta tctgctctca tcgatcggat tagggtatct gcggctcggc
     3181 caggcggcca acactctgtc cggtggagag gcccagcggt taaagatcgc cgcggaactc
     3241 aaagatccgt cggcacacaa ccagctctat attctggatg aaccgacgac cggcctgcac
     3301 ctcgacgaca tcaagaagtt gctggcggtc ctccacaaac ttgtggatgc cggcaatacc
     3361 ctcatcgtcg ttgagcacaa tctcgatgtc atcaaaacgg ccgactggat catcgatctc
     3421 ggtccggaag gcggcgaggc cggaggccag attgtcgccg aaggacgacc ggaacaggtc
     3481 gccaaggtgg cgcagtcgca tacgggacgg ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnccgaagga cgaccggaac aggtcgccaa ggtggcgcag tcgcatacgg
     3661 gacggtttct ggcgaaggta ctggaaagtt ccgctgtgcg cgcaacctga gaggcgaggc
     3721 atgcctgcaa tgagtgggag agggtaacag tcagcgaggt ggtatcagga acgccggcag
     3781 tcgtgatgaa cgccgctcgg cgcggctaat tggaggaggg atcgaagggc atatccgcgt
     3841 agtggcgaga ggtctcatca agcgacaaca cgatatccga ccaaatgcgc gccgcttcct
     3901 tctcaaacac aatcgaagga tcagccggca gcgtaatcca cgaaccggtc tgcatttccg
     3961 actccaactg cccaggtccc cagccggagt atcctaagta ggcccggaat gattcctttc
     4021 cgggctgctc catgagaatc cgttccatga tttccaggtc gccgccgagg cagacgccat
     4081 cgaatacctg atgggaattc tccggcgtgt cgctgatgcg atagagcatc atcacttgat
     4141 tggtttgcac agggccgccc gagtagagca cgtgtggttg gccttccagg atgggaacct
     4201 gtggcaacgc ttcagagatg gacatggccg tcggacgatt gacgatcacg ccgagcgctc
     4261 cttcaggccc gtgttcacag agcagcacga ccgcctgcct gaaattggga tcattcaatg
     4321 ccggcgtcgc cacgagcagc acgcctttag tgagtggagt tgtcatggtt catcctaccg
     4381 tgaggcatga aggggcgcaa gtcgagacag aaaattcaag cgaccttcgc tcagacccca
     4441 tacatggccg ggcggcggtc ccgcaggaga tcattatagc tattgagcgc ctttcgcctc
     4501 gcttccgcag gatcgatctc gatgatggtc agctcttcac gatcgcgggg cgcacggtgc
     4561 aggatttgac cgcgggggct gacgatttcg ctcagaccga taaacgtcag ccggtctttt
     4621 ccgccgcgtg cttcgctgcc gatccgattg gccgtgacgc tgaacacccg gttttccaga
     4681 caccgtgtca ccatggaatc cggacagttc ggcagaacca ggttggaggg atgggcaatg
     4741 atctcggcag ccagcagtgc cagggtgcga gccgactccg gatagtacca gtcgaagcag
     4801 atcatcacac cgattttggc tgcaccgata tcccagacct gaaagccact atcgccggga
     4861 ttgaaaaaca gcgtttcctc gaaaaacaga tgcgtcttgc gataacagcc gatgaacccc
     4921 tttggcccca ccacgacggc ggaattaaaa tatttcgtcc ccgcccgttc tgccaatccg
     4981 gcgacgatgg tggtcttgcg gcgcgcggcg atctccagga gtcgttgggt tgtcggccca
     5041 tccggcaccg cttccgcaag ccgagacact tcttcctgtg acgtgaactg atagccggtc
     5101 gcaaacaatt ccggcagcac cagcagatca gcctctgcgt gctcgagctg agctgtcaca
     5161 gtatcgaggt tgtgcgcgac ctcgccgaac accggatcac cttgcagata tccaacccgc
     5221 atcatgctgc ctttctacac aaagaaaaac gggcagggat gcccctgccc gttttatcgt
     5281 acgcgtcgtc gctgcgcaag aaccgcttac ataccttcgg acagatgggg aagcgcgttt
     5341 tcgaccgctt tggtcgccac atccgcatgg cctgccttac cgtgttcgat cccgtccttc
     5401 aagcccttga tggcttcacc cacatgagga ttcttggtct ccgccatggc gccttcggca
     5461 tgcttgagcg ccgcctcagc atgtttcacc agcgcatccg catggccctg cttcccatgc
     5521 gccaccgctt ccttggcatg ctccacggct tccgcttgat gcttattccc cgccgcgaaa
     5581 gccacgctgg ataccatcgg cgctccgacg agcgccatca tgactccggc caccacgact
     5641 ccccgccaca tagcagtctg catgaaacgc ctcctctgta agtatgtgca ctgcatcaat
     5701 ccgaatacat tctaattcag cataaagacg ggcccctatc ctgtcaagac ggacgcatgc
     5761 cggtcggcac ctgcgactcc gacaagccga gcagtttttg caaggcaagc agatccttct
     5821 ccaccgggca ggcgaaatcc cggctccact cccaccagga gcccggataa ttacgcagcc
     5881 gctcaaagcc cgcgaggcgc agcacaaaat agagccatcc cgaccggacc ccgcccgtgc
     5941 agtaacacac cgtttcatgg gccgggttca accccttgag ttccaactgc gcatgaatgg
     6001 cgtcgagatc cttcaccgtg gcatccgcat tgaggaatcc gctccatgcc acatgcaccg
     6061 cgccggggat atggccgggt ctcggaatgc cggacacttc cttcccgaga aactcttcca
     6121 gactccgcgc gtccacaatc gtggtggtcg gatggggctt gcgtacgata cccttcaatt
     6181 catccttcat gatgatcgcg gaggccaccg gcgagacggt gaagttgcct ggcgtgggac
     6241 ggaccgcgcc atgctcgaac ggccggcgct cgtgaatcca cttgacccaa ccgccatcca
     6301 ggactttgat gtttttgtgg ccgaggtatt ccaacatcca aaacatgcga ccttcatcac
     6361 cccagttatc gaaggggttg gaatagatga cgacctcatt gtcccgatta attccgagtg
     6421 cttgcacccg cttctcgatc cgtttcacat ccggatccaa gaggcctttg gcgatcgcct
     6481 cgggatcact gtattcgtgc caggtggaat gcacggcgcc gggaatgtgc ccgccgaact
     6541 cgtacgtgga tcggccccgc acatcgagga tcaccaggcc tggctgccct aaccgtgtct
     6601 gaagtgtctc gcaatcgatg agtaatggat gttgcatcgc gtatcctcgg tgagtgatta
     6661 acccggccaa cagcttaccg aaccctcacc aatgaacaca acaggactat tgtgaaacgc
     6721 ggaagggatt tcattggcgt cgatacgaaa actcacaggt cggtcgttga gccttcctac
     6781 actgatgtgg tgcataggca tttccttcca aggtatccac ccaggtgtct tagcatgatc
     6841 gatatgctac aaaaacctga tgacgccaga atgtgaccat gagtaccaac tcgagtgcgt
     6901 gggcgacgtg tacctgactg gttcgtatgt ctgtcggctg tgcgactttc gaatcagtat
     6961 gtccgatgag cagttccgca agcatgtacc gtcttcgccg cactaccagg caaacgcaga
     7021 taaaacagag ccggaacgca cggaaagaaa cagctctcct gcggttcacc gttcccgcaa
     7081 gatccctcct acctgcctcc tgctcgccca gattgatacg cttatcgagc tttgcgccat
     7141 cgggatttga cagaaaatac cctatgcgcc gcgcatcgcg cgtattctca aagggtttgt
     7201 ttcgtccaat taatctggcc tccccgagca aggaaaatgc acgtgccagt gctttggcct
     7261 ttacaatgat gagcggaggc tgatcaacgg tcagcccttg caggatatcg agtgcgttta
     7321 atggtcgcga acggatgtgg tggaggttgt ccgatcccgt tcgatgacgc cttagccttg
     7381 tggagctgat ggattcgcgc aggccatcgg tggcggctca ggcaagggat cagcgaacgc
     7441 ggccgcgagc tgttcgttca gcggcacctc tcgttcatac accgtgaagg cgtagggatc
     7501 gtccgccatg agaccgtatt tcttcttcaa catctcatgc tgaccgtcgg tcagaatgtg
     7561 gtaccgaacg cgagccttca aggtcacccc cttcgatgct tccggcatcc ggtaggcgaa
     7621 ttgatattcc cggctcgcca gcggcagcaa gcggttgtcg tacaactcca caatggccgg
     7681 ctgccacata atccagcgac ccatggtatg gctcttttcg gccagaatct gctgattgct
     7741 gtccttcacc gtaaattcga cggtgaagtg ccggtccgga tcgccggtgg gaagcttatg
     7801 tcccgctccg gcattcgtga gggtcagggc tatcgtgacc tggtcgcccg gcttgggctt
     7861 gggaggatcg accttcactt gaatcgcggc cgcccgcttc accatgtcgg gatcatgacc
     7921 gccgcgccag agatgccggc ggccccgcct gatgggtccg ttctcggcca cggggcgatc
     7981 cacctccggc atgtggcagc tttggcagat aaagccccgc tcctgcatga aatattttcc
     8041 ttcatactcc gcatacgtgc cgcagggccc cacattgtag aactgcgcag gacctgagac
     8101 cacattgtgg caacgcgaac aaaactgcgc ggtgcggaac gtcgggtcaa atttggtcgg
     8161 gtgcggcgca gccgaatcgt cgaaggggcc gaggatgacc ccatcgcgta catggcaggc
     8221 cgcgcaggtg atggactctt tttgcagcgc ggcatcgaaa tggggattcg gttcctgggt
     8281 cgccttttcc acgcgccccc gcgggatatc tttcaccaag gtcggctgtt ggttctccag
     8341 cggggtatgg caattcaaac agacccagac ctgtttatct ttcttccagt acgcctgaaa
     8401 aaagggatct tcataggcgt gcgcatgaat gctcgtcttc cactcctcat aaatctcccg
     8461 gtgacattga ccgcaggatt cggccttgag gctcgtcaag ccttcgggaa tcttctggaa
     8521 gggaatcgca tgcgcatact cggaccggag cccgaaaatg acgaccggct tcacctccgt
     8581 gtaatagagg tacaccagcc ccgagatgac cgcgaggccg agcaacagct tcagccacca
     8641 tttcatgacg ccaccagtgc ccggatgtgc cgctcgaccg aagccgccaa ggccgtcagg
     8701 ttatagcccc cttccagcga ggaaagcagt ctcccccggc agtgccgagt cgcgatgccg
     8761 gccacaatcg aggtcaagtc tgcgtaccct tcttccgtca ggcccatgct ggccagggga
     8821 tcatcccgat gcgcatcaaa gccggcagag atgataacca actcaggctt gaaggcatcg
     8881 gcggcaggaa ccagcacttt ctgaaacacc gcccgatagt cgtcgtcgcc ctcacccgct
     8941 tccatcggca cattgatggt gaagccttcc cccgcgccgg tgccgcactc cgtcgcgcga
     9001 ccggtgcccg ggtagtgcgg gtattgatga gtgctgaaga acaacaccga ggggtctgcc
     9061 tcaaaactat gctgcgtgcc attgccatga tggacatccc aatcgacgat caggacccgc
     9121 tgcaccccat aacgtttctg cgcgtagcgc gccgcgatgg cgacattgtt gaagagacaa
     9181 aaccccatgg cccgccctgc ctccgcatgg tggcccgggg gacggaccgc gcaaaagaca
     9241 tgctggacct ggtcggccat aatggcatcc acgccggaca aggccccgcc cgcggccaga
     9301 taggcggcga ggagcgatcc gggggacatg gaggtatcgg catccagaga tacccggcca
     9361 ctagtcggcg cttcgcggtt gagatgcccg acatactcgg ggcgatggac caacgtgatc
     9421 cactcatcct cggctgttct cggtgctaga cgggtgagtt cagcagccaa cccgctttgc
     9481 tctaatcgct gcatgattgc acgaagccgg ttgggcgact ccggatgtcc ggcgcccata
     9541 tcgtgggcga gatagcgagg atcgtaaacg agaccagttc ttcccattga gtactcgcgc
     9601 cggggaaacc gccagcgaca ttgccgacga caagaggtct tttgacctta acacgcgtca
     9661 aactgcgtag acaacgcgcc gagaacgctg ttatcgtcct ccaaagggag gactcgatgc
     9721 cgaatccacg aattgaaccg ctgaaacgag tgttagccat ggagccggac gatgatgtcg
     9781 cctggttcgg gctgggaaaa gcctatatgg aagatgccaa cttcgaggaa gccgcgaagg
     9841 ccttgaggca gtgcatcacg gtgaaaccga cctactcggc tgcgtactat gccttggcgc
     9901 aatcgctatt gaaactgaac cgtgtggagg aatgccgcca ggtgtcagac caggggatcg
     9961 acgtgtcgac caagaatgga gacgcgatgg tgacaaaaaa ccttgaactc ctgaagagct
    10021 cgctccccgc cgcctgattc caggctcggt gattcgtcag tatcgtgcgt cgcgaggaag
    10081 actgcgcagt tgacggcgga cggatgcctc aggccgatgc cgcgccggtt tcgtcatcat
    10141 cgccggcatt ggcctcggct tcacgctgct cggctaaggt ggtcgccacc tcatccaacc
    10201 aacgaccggc cccatccagc acttcgcgta ccagcggctc cggatggccg gtacgtttca
    10261 tggtccactg acagagatag cccatcaaat cttcccgttc gaaccgccgg gtcgattgtg
    10321 tcgagagttg agacaactcg gacaggccgg tccggagaat ctccgccacc gtgtcccggc
    10381 cgtaggacgt cgccgctgtc acatattgaa tggcccgatt gatctcttgt tcggtcatgt
    10441 ccccgctccc caccatggcc ggattgtaac attcaagcgg gacctgtcaa cggtgcatca
    10501 gcccgaacag cctgccctca aagaatctac acgccctgag ccggcgagga tggtatgatt
    10561 ccggctatca ctcatttgta ggagccccga ttatggccgt ttcccgcaag actgcatctg
    10621 caacagtatc atcccgacgt tccacgacat cccgcgaatt tgccgaccgg tgggacctgg
    10681 ccgaccttgt cgccgatccc gtgaaacaat tcgatcgata tctgaaagac ctgacagcca
    10741 aggtgacgcg ctttgaagcg gcacgcccgg acctctccgc aaccatgtcc gagcaaagct
    10801 tcttgaatct cctgaccttg tcggaacaga tcgcgcgcga ttccggccgg ctgggagcct
    10861 actcctatct ctggttttcc gaagatacca agcagctgca ggcccggtcg ttcaagacca
    10921 aagtggaaga gcacctcacc agtctccaga atcgcctgtt gtttttcgat ctgtggtggc
    10981 aaagcgtgga tgagaaaaat gcggagcgct tgatggcgaa tagcggggac ttccggtatc
    11041 acctggaaac catccgccgc ttcaaaccgc atacgctctc ggagccggaa gagaaaatca
    11101 tcaacatcaa gaacatcacc ggacaaagcg ccgtgcatca gctctacgat gtggtcacca
    11161 acggcttcac cttcacgctg cgcatcggcg ggaagaacaa gaccatgaac cgcgaggcgt
    11221 tgacggccta tctgagaagc ccgaaagccg ggatgcggga agccgcctac cgggaaatgt
    11281 atcgcgtgta cgaatcccag caggatctgc tcggagagat ctataagacg ttgatcaatg
    11341 attggaaggc cgaaaatctt caactccgcc atttcaagac accgctggcc tcccggaacc
    11401 tcagcaacga cattcccgac tccgcggtgg aggcgctcct agccatctgt atgaagaacg
    11461 ccccgatctt ccagcgctac ttcaagctca aagccaagct ctgcaagatc agcacgatga
    11521 accgctacca tatctatgca ccgcatcgcg ccgagcaaaa gagctaccgg tacccggacg
    11581 cggtccgcat ggtgctagac gcctattacg gattctcgcc gcgtttggct gaactcgcac
    11641 aacgcgtctt tgccgatcgg cacatcgatg ctcggatgaa ggcgggaaaa atgggcggcg
    11701 cctactgcta cagcgtcacg ccgaatgtaa cgccctatgt catgctgaat ttcactggag
    11761 aggcgcgcga cattgcgacg atggcccatg aattaggcca tgccgttcac gccatgatgg
    11821 cggagcagca caacgtgttc actttccact cgacgctgcc gcttgcggag acggcctctg
    11881 tgttcggcga gcgcattctt tcagatgcct tgatggcctc ggagtcgagc aagtcggtga
    11941 aacagagcct gctcgtgaat cagttggacg acatctacgc caccgtcatg cgtcaggcgt
    12001 actttgtcgc cttcgaacgg acagcccacg acatggtcgc ccagggcgcc acaacgaatg
    12061 atctggccgg cacctatatg accctgctca aacagcagtt cgggaaatcg ctgcgcgtac
    12121 cgcaggagtt ccaatgggaa tggctcacca ttccgcatct gtatgccagc cccttctact
    12181 gttatgccta cagcttcggg aatctgctcg tgttggcgct gtatcgcatg taccaggaac
    12241 aaggcgccgc attcgtcccc aagtacctgg acctcctggc ggccggagga tcgaaagcac
    12301 cgcaggcgat cctggctcag gtgggtgtgg acatgaactc ccacgcgttc tggcagtccg
    12361 gcttcgatgc gatttcagga atggtcgatc agctggaaga gaccatgaga tgagcgacag
    12421 gcaggagcgc cgggtctaac aaagacgcgt ccgggatcac attggaaggg ctgcagacaa
    12481 ccagccggca aggagaacta aatgcgttcg gaagatttgt ccatctcgac cggatgcccc
    12541 agaatctctc gttccgcttc caaccagtcg cggagatgat agccgtccag gcatccccgt
    12601 tgttcgtaca gctcgtgcgc cctggccgca atccgcactt gaatatcctg cacaggtcca
    12661 ggctgcttct cggacacagc tggttttgca acctgctttg tcgtgcgtgt ggcggctttt
    12721 ttcggggcgg tcttcttttt cataccttgt tccctcatgt cctgtcgcca aacggtgcgc
    12781 ccgacatttc gtgaggccac tcgtcggcaa tccctgttcg caccataaca tattccgcac
    12841 ggttcctgta ccgcagcacc accttgcctt cccggctgag ccgatccacg gtgagaaaca
    12901 gttggttcgc tgtgaacgtc ggcacatgac ggaacaattc ctccatcgaa catatccggc
    12961 agcgctgcaa ataggcccac accgcggagt ctacatcgta gccgtcattc cggtcggttc
    13021 catcattcat tgtttcgcct ccctcgacta ttcgtcgtgc atgaggatta cgcaggccag
    13081 tcccgcgctg agaccagcgt gcgaacccac ctaagattgc gggcaccgct atggcatctc
    13141 atccgcccgc ttgtcgatct gatctgcagc agctcgcagc tgtcgagcaa gggcccgccg
    13201 ctgctggatc aaccgcgcat ccgtcggcac ggggtgctgg agcaatactt cagcttcgac
    13261 ctcatggcga tcggcgaatg tccgcagctc ctgcgcgtct tcatgccact ggagacggct
    13321 ttccgatggc cctcgcgtca tccccatcac agaagcatcg gacaaggcgg ggaagggttc
    13381 ccgaaccgac gtggccacac atccggcgag tgtactccat gcaagaatga cacatgcgtg
    13441 tgatagcaga gtcttcatga tgagcctcct ctgtaccgtc agtcatcgcg tcacgggacc
    13501 actgagcgaa gcaagatatg atcctgcagg tgaaaggcca ggcagcgtca aacaaaccgt
    13561 gattgctcta tctcagatct ctatggaggg agggtccatc acagaggacg gataccgtat
    13621 ccagatttcg caccgctcgg cccgtgcgct atcgcgccat ctggatgccg gaatgacaga
    13681 gtgactcatt tggtaaggat gaccgcctag ggatgcacca ggcacgccag gttgtcagcg
    13741 cgccgggaca tctacgcatc atcgaatgaa agggattgag attacgacgc cttggaagtg
    13801 atccggtccg aggcctgctc gctttcgggc aatgagagcg actcatgcaa ggcttgcaat
    13861 aacatttgcg gagaaaaggg tttcgaaacc aggatgcacg cgccctgctg tttgagggcg
    13921 gcggggagtt ccagcaactc cgcggagagc aataccaccg gaatgtcagg ccacatgagg
    13981 cgacagagca ggagaaattg ctccccgtcc aggcgaggca tgtgaaaatc agaaatgacg
    14041 gcatcgaagc gccgcttctt catctcgccc aacccctcga cgccatcgac ggccgcatgg
    14101 acgatatacc ctgcttgctc cagcacgatc gcaaccagcc gtcgtacact ttcttcatca
    14161 tccgcgatca acacgcgctt cccatagccc tccatggcgt ctccttcctc tggtgcatcg
    14221 caacagttca catccctcca cctgtcgcac tgacggacag ccgattcatt ccttttgccg
    14281 cccgcggatc tcctcactcc agacgcccct tcgttgcgga cccggcctgg tgcggcgccc
    14341 cagacgtccg gagatgccac gagccaaccg gatcaacgca tcttccagat tgccgcaatt
    14401 gagacagcgg acgaccggca cccaggtatg gctcacgccc tcgcttggat tgaacagcgt
    14461 ctcgtccacc atcaagccct gacatcggct gcatgccatc cgtggcctcc ttcgatcaat
    14521 agccgtccga agcgatctag cgaccccgcc ccatcaactc ctgctctgtg cgcatgagac
    14581 tctcaggagt cgacctcgtc ccgcgatcct gatcgggatt gatcagaatc ggcacgcgca
    14641 actgatccgg cagcagcacc attttcgcgt tggggctgtc atagagtttg tattgcaaat
    14701 attcaggggt cagggttttc gtgatcgttt gctgtgcatt cgcctggcca agcgctcgaa
    14761 tcttcagccc ttccgcctcg ccctccgatc ggattcgaat ggcatcaccc tcgccgcgcg
    14821 cacgccgcct ggcgatctcc gcttccttct ccgcgatgac gagttcgaat tccttttgtt
    14881 ctttttcctg ctccttcgct tgcttgcgct ctacggcctc cagcacgatc ttggctaact
    14941 caatatcggc gagcgccacg ctgtgcactt ccaggtgacg gcccttgagt ttctccacca
    15001 caactgcctg cactttactg gcgatttccg cgctcatttc cgggaccgcg accatcggat
    15061 agttcgacac gacgctacgc accgcggcca gcagttccgg tcgaacgacc ctcggataaa
    15121 aatccgggcc gatctcctgc gcgaggaaat acacttcttc ggcaatcgga cgcatgatga
    15181 tggctgcctt caacttgacc aacagatcgt cggagctcaa cgcgtccacg agttccgtgt
    15241 aactctgcaa acgcacgtca tagacgtaca cgtcgttcca gggtgctctc caatagaacc
    15301 cggacttcaa ggtctcggtg gtcagtccct cggtcaaggg gtaccagcgg agaccgcgct
    15361 gtccagggct gaccgtgttt ccacaaccag acaacccgca cagccctgca atgatcagtc
    15421 cgatcgtcgt atatcgccac atggtctccc ccttcctgca ttcatgtcat tcagcgggat
    15481 tcctggtcag cagcatggga acgccggtca tggaggcgct gatggcgagc ggctatccgt
    15541 tcttgttcct gtgccgcagc ctcatacgat tgtgacaaga gtcgcgtgcc ttcgacccaa
    15601 tccgagtgag ggccaaacaa ccgttcgtac acggccacac gatggtcata atcctgcgcc
    15661 atctgccgca gcctcgtcgc ttcgtgtgca tagaactcag cgattttttc ctgatcctgt
    15721 ttcggatcgt ttgtctccaa atcgaacacc gaggcggtgt tcccacaagc aggcatctgg
    15781 caggctgcca ccagcaccag acatcggaca acagcgggac acgacacgcg catcatcgca
    15841 cctgcagatg cccccccttc gacaacaggg gttcggtctc tcgaccactc cgccaaatca
    15901 cttcgtaatc cgcagcgagg ggcaatagtt cgacctgctc ttgtgtcctc aaccggttca
    15961 acgccgcaaa cagggattgc caggtataca acggcagcgc atctgccaag gtgagaaagg
    16021 tcagtcgccg gtggcggtgt aacaacacga gaatttggtt gtcgatcaca tcgtcactcg
    16081 gaaaaccaga ttgacggatc atcgcccctc ccttgcgcac catctgcgag gatgcgctgt
    16141 tgaattccgg tccaaggaag tggctttgca atatatggtc ctgtcaaaag cggagacgaa
    16201 gctcaggtaa ttgctgcgct acgcgatggt accgaatggc catcggcacg gtgggccaac
    16261 tgggaacgca tttcgcacgg tacatctcgt gcggaatcgc tcgagtcgaa ctgttttttt
    16321 gaatcactta caatggaata tgagggttcg ctggtgattc cggttggcat tggcattgct
    16381 gctcacatca ggactagaga cggcttggac ctgaggtaaa atcgccgtgg ttcatatcaa
    16441 aaggatctac agcgaaccgg atccacagga cggaattcgc attttggtcg accggatatg
    16501 gccgaggggt tgcacgaagg agcgggcgcg gctcaatgac tggcaaaagg aattggcgcc
    16561 cagcaccgcg cttcgcacat ggtttggtca tgatccacgg aagtggaagg agtttcgctc
    16621 gcggtatctc gctgagctgg cacaaccggc acaacgcagg gcgatcgaag aactcgtggc
    16681 gctggctcga tcgcagacac tgacgctgct gttcgggtct tcagacgtga aacacaatca
    16741 ggcggtggtg ttaaaagaac tgatcgatcg tgcagagaga gaatgacctt gcaggcaact
    16801 ggaaaccgga accaccttca cgcgcatctc actgagatca gatacgctca cacgggtagg
    16861 cggataccct ttcagcaaac ttagctggca agcccccagg tgctccacag cgcaacggct
    16921 gcaacagcca acatcaccca ggccaactcc cgatctccaa ctttcttgat gatctttacc
    16981 atggctcatt cctttcgctg ttccgatcac gctcactgtg atggcagaca ttaaagcaag
    17041 gcgtatgcca tcacga
//
LOCUS       DAOQDS010000078        16199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1495_length_16199_cov_19.051784, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000078 DAOQDS010000000
VERSION     DAOQDS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16199
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1495_length_16199_cov_19.051784"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PK589_15630"
     CDS             <1..1077
                     /locus_tag="PK589_15630"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ21090.1"
                     /translation="ARLFPNPVAQIGTVASFTHGNTAANSGALTMQVQQLFELAGKRG
                     YRIESAGFGVQSAEADFEDAIRQLGFTLKDAYYRVLVAQRRLALAEENRDRFARILDV
                     NTIRFKKGYIAEVDLIRIRLQVVDFQSQVIESIQEGESARADLRQLLRLSPASKLELT
                     TEMDYRRVDPDMGKLRSVALDVRPDIRSRRALVAQREADLKLAKAFRVPDLTIGAGYS
                     IQGPRGPDNPQMGILNLGVPLPLFNRNQGGIVQAEVGVQSAQAELDRTVNQVENQVDV
                     AYHNLLQSRRLVEAYLAGVLEDARSTFTIVERAYERGGATILDLLDAARTSRTIQQNF
                     IEALFSYQRNLFQLESSVGQEITS"
     gene            1074..2222
                     /locus_tag="PK589_15635"
     CDS             1074..2222
                     /locus_tag="PK589_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ21091.1"
                     /translation="MNSGAIPVIGLTVVLLFSACSQKQDSAPSPPSASSEPVVAPAVP
                     PSAGQIETAVVDFKPVQPELVLVGKIAYGEDRYSKISSPLQGRVVEVRAKLGDRVEAG
                     ATLLVIDSSDITAAYSEFVKEASELEYSTRAQELAKELYATKALALKDLKQAENDLIK
                     ARAEFRRAKERLLSLRIPAAELEKPLAQQRITSRFEMKSPLTGTVVERAVTPGQSVGG
                     DVGQVLFTVADLDRLQVVADVYERDLALVKVSQVGRINVEAYPGTDFASVVASIGDVV
                     DPNTRTIKVRAWVDNRDQRLKPEMFARLRLDIGDATPFLTIPKEAVVEIDGKHFVYVV
                     DGADHYSKHEVTVSNVSSGQVRILEGLTSGQRIVTKGAVLVKGQEVKG"
     gene            2272..5376
                     /locus_tag="PK589_15640"
     CDS             2272..5376
                     /locus_tag="PK589_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNJ21092.1"
                     /translation="MMVRIVELSLVQRFLICALGFTLLFGGLYAFQQLDIVAYPDPSP
                     PMVEVITQFPGWSAEEIERQITIPIEVALNGMPGLTDIRSLSIFGLSDIKIYFDFNTQ
                     YFFDRQEVVNRLSTLSLPQNVQPALSPWWAIAEIYRYQLEGDGKASLTDLKTIQDWQL
                     RREFRRVPGIIDVTAFGGTTQEYHVDVDPGKLISYQVSLAQVMEALTNSNANVGGNYL
                     TVGAQNYNIRGLGLINGLADIENVMVAEKEGTPIFIKTLGSVSVGHRVRLGKVGIDDN
                     DDVVEGVILLQRGYKALSVLDRVREKVEELNKWKLPEGVKIKTFYDRTALIHTTVETV
                     TDILISGMVLVFIILFVFLGHLRASLIVALTIPMSLLFTFTMMVMIGQSANLISLGAI
                     DFGIIVDATLVMVESIFFHLGHDRRLGLTVPQQIVRAARQVGQPIFFSTAIIVVAFIP
                     LFTMTGVPGKIFAPMSITYGFALFGALLMAFTLAPVLCSLLLTGVVTEQDTRFVSAIR
                     RTYLAVLRWALAHNMKVIGAALLLLVAAFGALQFIGGEFMPALEEGNLWVRATMPVDI
                     SFDEAARLTGEIRQMFRKSPEVVTIVSQLGRPDDGTDPTSFFNAEFLANLKPHKDWRT
                     GLTKDQLVEEIEERLKVIPGIIFNFSQVIQDNVEEAMSGVKGENSIKLFGTDLKTMEA
                     KAGEIESVMRGVHGVKDLGIFRLVGQPNLLIQVDREASARYGLRVSDVNAVVQAAVGG
                     QAVTQVFEGERLFDLVVRFLPEFRRDEEAIGNILVNTPNGARIPLKQVATIKTQTGAF
                     IIYRENNERYIPIKFSVRERDLESTVKEAQAKMAQAVSLPERYRIEWAGQYDQLTAEQ
                     KRLTMIVPLSLVIILFLLYTTFNSLTNALLVLVTVPFALIGGIFSLVFTGTHFSISAA
                     VGVISTLGVAILGGVLLISRIEDFRRSGLELREAILKGADVQMRPILMATLAAAIGLL
                     PAALATGIGSQAQQPLARVVVGGMLTAAALILVVLPVLYEVVHRRTAPRTPLEAGEEP
                     PAPY"
     gene            5404..6207
                     /locus_tag="PK589_15645"
     CDS             5404..6207
                     /locus_tag="PK589_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ21093.1"
                     /translation="MIGVGQQRMRQVVVLGLILLGLMGMRPEAGWAGSMVWPVQMPYE
                     APPKSEPVPDVSIAPNTKALTPEELQRAESLLPLLEGKQEFWAMGEFVHLGEPALPVL
                     TKALTMPGPRIRYNAIETMSMIKAPAATPALLEAAKLATEIPRIREHALRVAVRLDPQ
                     QAPPAIEAMAKDPNSVIRKAAAFEARYVRKKEVIQPLIDLLGDEEKFVAMSAVQSLWT
                     LTRHETEFHDWDASNKQDRQNWAQEWIEWWNQSKETFEIPDPKTRKQAG"
     gene            6318..6848
                     /gene="def"
                     /locus_tag="PK589_15650"
     CDS             6318..6848
                     /gene="def"
                     /locus_tag="PK589_15650"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNJ21094.1"
                     /translation="MAILAISKLGNPILRQQAQSVSPSEIKKAAFQQLIDDMFETMYD
                     EPGIGLAAPQVGRSQQLVVMDCPGEGGFPKTVLINPTIRFYGPEQVEGWEGCLSVDGL
                     RGKVTRPSTVRVTGLDRNGKPFDFEASGLYAVCIQHELDHLIGKLFIDRMTDFSTLTQ
                     MDEFQKYWQKEPASVI"
     gene            6918..8597
                     /locus_tag="PK589_15655"
     CDS             6918..8597
                     /locus_tag="PK589_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ21095.1"
                     /translation="MLAVVTFVCAGLATALELVPPYLVKIVIDDVIQAKRPEMLMWVL
                     GALLGSYILRNLMSSMRVRFNNLLEQKAVHTLRMQVFGALQRLSLSYFENRSTGEIMT
                     RVTSDTEHVERIFVDGLEGLLTASLTLIGITIMMFFLNWKLALLSLVPIPILIVCAAW
                     FTKRVHKYYAEIRKSVADLNAYLQDALSGIKETIGFNQHEYERKRFETLSQTYSQNSL
                     RAMFLWSWYWPGMVFVGSTGTVLILWYGAEEVMAGNLTVGQLVMFLSYLGLFYTPINE
                     IHSLNHMLQHALAASERVFEILDTKAEVEDRPGAVRPVERLRGAVEYRQVVFGYRQEG
                     TVLSDVNLTVRPGERIALVGPSGAGKTSLLKLLMRFYDVRSGAVLVDGYDVRDLPVAF
                     LRGQIGLVQQEPFLFNGTVRQNIVYSDLSASHERVEAAARAARAHDFITRLPDGYDSW
                     IGERGVKLSVGQKQRVSIARVLLKDPPIIIFDEATSNIDTETEVKIREALNDLTKGRT
                     TFIIAHRLATLHDVDRIIVVERGTIVEEGHHDALMKRGGVYAGLYEAQFKI"
     gene            8780..8974
                     /locus_tag="PK589_15660"
     CDS             8780..8974
                     /locus_tag="PK589_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21096.1"
                     /translation="MVLIFESDPLDNEDARGRFYHVCRGRIDRTEITLPQGQSIYECA
                     VCGIDLEPEDFWIARQKGSV"
     gene            9016..9225
                     /locus_tag="PK589_15665"
     CDS             9016..9225
                     /locus_tag="PK589_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21097.1"
                     /translation="MAGSIGRKTVSVSRGLMMLCETCYDQVYAEKLPDARNFIADHEA
                     LFDTICMGCTDINRPLIDDMLGSSE"
     assembly_gap    9226..9325
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9604..10008
                     /locus_tag="PK589_15670"
     CDS             9604..10008
                     /locus_tag="PK589_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21098.1"
                     /translation="MKPLVAINGASMFGELAIVSCLEKDGWTAFWVDTFHGRKFWRGM
                     PHVTQPTEPPTKVKALYARIADRKGGPSGCFDIVASKGDRIIWLEYKGPKDTANRNEL
                     CWIEAALGAGVAESSLFFVGSDRRPKAVERTI"
     gene            complement(10118..10447)
                     /locus_tag="PK589_15675"
     CDS             complement(10118..10447)
                     /locus_tag="PK589_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21099.1"
                     /translation="MRLLLVMIALILCLTGLTHPGTSSAAEKSYYSPIINVDVDNSRI
                     LISTLGAVFWVDVPEEAKAHIEKLPQSGLVDIVVETREGQPPLLKTWKVKSGESTCLH
                     FDGKTCK"
     gene            complement(10546..11058)
                     /locus_tag="PK589_15680"
     CDS             complement(10546..11058)
                     /locus_tag="PK589_15680"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNJ21100.1"
                     /translation="MPDLSIPQHILDQIVAHARELAPFECCGLLAGKNKVVTHLYRIT
                     NIVAVEGAEKLSTFDEDKVAHLERLSPEERAEIAFVMDMGDFSAAKKDIRKNGLDLQV
                     VYHSHPKDPARPSHTDIKIATDYEEIWQRINLPVPAYLLVSLMHAPAADIRTYWITGG
                     QVRPADIHIM"
     gene            complement(11060..11863)
                     /gene="moeB"
                     /locus_tag="PK589_15685"
     CDS             complement(11060..11863)
                     /gene="moeB"
                     /locus_tag="PK589_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNJ21101.1"
                     /translation="MEFTDQQIQRYSRHIILNEVGGKGQVKLAKAKVLLIGAGGLGSP
                     AALYLAAAGIGTIGLVDGDVVDLSNLQRQILHTTATVGVPKVESGRRMLSAINPDITI
                     KTYQLNVDTENILGLVSDYDIVLDGSDNFTTRFLVNDACFFAKKTLISASMFRFEGQL
                     TSIKPHAGFPCYRCLYPEPPPAGLVPNCQEAGVLGVLAGTMGILQASEAIKEILGIGE
                     TIADKLVIYDALEMKFRKVSRPKDPRCPLCSATPTIKDLGGDYSVACTI"
     gene            complement(11939..12178)
                     /locus_tag="PK589_15690"
     CDS             complement(11939..12178)
                     /locus_tag="PK589_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HNJ21102.1"
                     /translation="MTSLRFHIRFPENKIKEPIIYQIGHEFRVVTNVRRADVRETTGW
                     MDLELTGETEEIERAIDGIRTKGCVVDPIELNVVE"
     gene            complement(12181..12453)
                     /locus_tag="PK589_15695"
     CDS             complement(12181..12453)
                     /locus_tag="PK589_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNJ21103.1"
                     /translation="MIKVRIPTPLRPLTKGQGEVESAAANIVDMIGSLDATYPGLKNR
                     LCDEKGDLRRFVNIYVNEEDIRFLNGKETSLKDGDEVSIVPAIAGG"
     gene            complement(12450..13700)
                     /gene="thrC"
                     /locus_tag="PK589_15700"
     CDS             complement(12450..13700)
                     /gene="thrC"
                     /locus_tag="PK589_15700"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNJ21104.1"
                     /translation="MSKMKALVCRECGKEYPPKAIHVCEMCFGPLEVKYNYDEIKSTI
                     SRKKIEQGPNSMWRYIDLLPVESTSIIGPHAGLTPLVRAKNLGAHLGIDELYIKNDTV
                     NHPTLSFKDRVVSVALTRARELGFETVACASTGNLANSVAAHAAAAGMKCYVFIPADL
                     EAAKVLGNLIYKPNVVEVEGNYDDVNRLCSEIAGEHGWAFVNINIRPYYAEGSKTLAF
                     ETVEQLGWRTPDQAVIPMASGSLLTKIWKGLHEMKALGLVDEVRTKINGAQAEGCSPI
                     ATAFKAGRDFFKPVKPKTIAKSLAIGNPADGYYALKATAESKGAMDAVTDEEVVEGIK
                     LLAQTEGIFAETAGGVTIGVLCKLVKQGLIKKNDVTVAYITGNGLKTQEAVVDAVGRP
                     FRIQPSLVNFQKTFKMGKNSGGES"
     gene            complement(13714..14541)
                     /gene="moeB"
                     /locus_tag="PK589_15705"
     CDS             complement(13714..14541)
                     /gene="moeB"
                     /locus_tag="PK589_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNJ21105.1"
                     /translation="MEFTETQINRYSRHILLPEVGGKGQKKIIQGKVLIVGAGGLGSP
                     AALYLAAAGIGTIGLIDSDVVDLSNLQRQVIHQTPDVGRPKVVSGKEKIQALNPDVNV
                     VMYEERLTAGNALKIMSGYDVVIDGVDNFPTKFLINDACYFAGKPLVHGGILRFDGRV
                     TTIIPKKSACYRCVFKKPPPEGLVASCQEAGVIGVLAGIIGTIQATEALKLILGIGRP
                     LTDRLLDFDARRTQFREIRIKRNPDCPLCGERPTITELIEDGDVGGPTCALPGQRNL"
     gene            complement(14693..15622)
                     /gene="cysK"
                     /locus_tag="PK589_15710"
     CDS             complement(14693..15622)
                     /gene="cysK"
                     /locus_tag="PK589_15710"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNJ21106.1"
                     /translation="MTAKAHGDITELIGGTPLVRLNRLTKPGSATIYAKVESFNPGGS
                     IKDRICLNMINEAERQGKLKPGGTIVEPTSGNTGIGLALVAAVRGYKLILVMPESMSM
                     ERASLLSSYGAQLVLTAAWEGMKGSIKEAESIVAQNPSYYMPDQFSNPANPAMHRKTT
                     GPEIVEALDGRVDAFVAAVGTGGTITGCGEVIRERNPAAKIVAVEPAGSPVLSGGDPG
                     PHKIQGIGAGFVPKVLNRTLLDRVITVTDDEAYQTAKLLAKKEGLLVGISAGANVFAA
                     QKIAEELGPGKNVVTILCDTGERYISIEKYFNI"
     gene            complement(15619..15819)
                     /gene="thiS"
                     /locus_tag="PK589_15715"
     CDS             complement(15619..15819)
                     /gene="thiS"
                     /locus_tag="PK589_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNJ21107.1"
                     /translation="MQVMINGKAEEIAGGTVLDLLKAKSIEPQMVAVEVNDTMLERTH
                     LETTQLKDGDHVEFLFYMGGGR"
BASE COUNT         3270 a         4442 c         4787 g         3600 t
ORIGIN      
        1 gcgcgcctct ttcccaatcc ggtggctcag atcggcaccg tggcctcgtt tactcatggc
       61 aataccgcgg cgaacagcgg cgccctcacg atgcaggtgc agcaattgtt tgaattggcc
      121 ggcaaacgcg gctatcgcat cgaaagcgcc ggtttcggcg tgcaatcggc ggaagcggat
      181 tttgaagatg ctatccggca gctggggttt accctcaaag acgcctatta ccgcgtgctg
      241 gtggcgcagc gcagattggc cttggcggag gaaaaccggg atcgcttcgc ccgcatcctg
      301 gacgtcaata cgattcgatt caagaagggc tacattgccg aggtcgacct gatccgtatc
      361 cggctccagg tcgtcgattt ccagtcccag gtgatcgaat cgattcagga aggcgaatcg
      421 gcacgggccg atttgcgcca gttgcttcgt ctctcaccgg cgtcgaagct ggaattgacc
      481 accgagatgg actaccggcg ggttgatccg gacatgggaa aactgcgctc ggtggcgttg
      541 gatgtgcgcc ccgatatcag aagcagacgg gcgctcgtgg cgcagcgtga agcggatctc
      601 aaactggcga aggcatttcg ggtgccggat ctgaccattg gcgccgggta ttcgatacaa
      661 ggaccgcgcg gaccggacaa tccccagatg ggcatcttga atctgggcgt gcccttgccg
      721 ctcttcaatc gcaatcaggg cggtatcgtg caggccgaag tcggtgtgca gtcggcccag
      781 gcggaattgg atcgcaccgt caaccaggtg gaaaaccagg tggatgtggc ctatcacaat
      841 ctcctgcaga gccgtcggtt ggtcgaggcc tacctggctg gggtcttgga agatgcccgt
      901 tcgacgttta ccatcgtcga gcgcgcctac gaacgcggag gcgccacgat tctggatctc
      961 ctcgacgctg cgcgcacgtc ccgtacgatc caacaaaact ttatcgaagc cctgttttcg
     1021 taccagcgga atctgttcca attggaaagc tctgtggggc aggagattac gtcatgaatt
     1081 ccggcgcaat cccggtcata ggcctgaccg ttgtgctgtt gttctccgct tgcagtcaaa
     1141 aacaagactc tgcgccttcc ccgccttccg cctcttctga accagtcgtc gctccggccg
     1201 ttccgccgtc cgccggccaa atcgaaaccg cagtcgtcga cttcaaaccg gtccagccgg
     1261 aattggtgct cgtcgggaag atcgcctatg gcgaagaccg atattccaaa atttcctccc
     1321 ccttgcaggg gcgggtggtg gaagtgcgcg ccaaattggg tgatcgggtc gaggccgggg
     1381 cgacattgtt ggtcatcgac agctccgaca ttaccgcggc ctattccgaa ttcgtgaaag
     1441 aggcgtccga gttggagtac tcgacgcggg cccaggaatt ggccaaggaa ctctatgcca
     1501 cgaaggcgtt ggcgctgaaa gacctgaagc aggccgaaaa cgatttgatc aaggcgcggg
     1561 cggaattcag gcgcgccaag gagcgactgt tgtcgttgcg cattcctgct gccgagttgg
     1621 aaaagccgct ggcgcagcag cggattacct cgcgctttga gatgaagagt ccgctgacgg
     1681 gcaccgtggt ggaacgggcc gttacgccgg gacagtccgt gggaggtgat gtcggccagg
     1741 tactgtttac ggtcgccgat ctggaccgat tgcaggtcgt ggcagatgtc tacgagcggg
     1801 acctggcgtt ggtgaaagtc agtcaggtgg gccgcatcaa tgtggaggcc tatccgggaa
     1861 ccgatttcgc gtccgtggtc gcgtccatcg gtgacgtggt cgatcccaac actcgcacca
     1921 tcaaagtgcg cgcgtgggtc gataaccggg atcagcgact gaagccggaa atgttcgcac
     1981 ggctccggtt ggatatcgga gatgccaccc cgtttttgac gattcccaag gaggcggttg
     2041 tcgagatcga cgggaaacat ttcgtctatg tcgtggacgg ggccgatcac tactcgaagc
     2101 atgaagtgac cgtctcgaat gtctccagcg ggcaggtacg catcctggag ggcctcacat
     2161 cgggccagcg catcgtgacc aaaggcgcag tcctggtaaa agggcaggag gtgaaaggat
     2221 gatcaggccg gtgatcgtgc gccactgccg acaacagcgc tctgaagcca catgatggtt
     2281 cgaattgtcg aactgtctct ggtccagcgg tttctgatct gtgcgctcgg cttcactctt
     2341 ctctttggcg ggttgtacgc ctttcaacaa ctcgatatcg tggcctaccc tgatccctcg
     2401 cccccgatgg tggaggtgat cacccaattt cccgggtggt ccgcggaaga gatcgagcgc
     2461 cagattacga ttcccattga ggtcgcgctc aatggcatgc cggggctcac cgatatccgc
     2521 tcactgtcga ttttcggatt gagcgatatc aaaatctatt tcgatttcaa cacccagtac
     2581 tttttcgacc gccaggaagt cgtcaaccgc ctctcgacgc tcagccttcc gcaaaatgtt
     2641 caaccggctc tatcgccctg gtgggccatt gccgaaatct atcggtatca actggagggg
     2701 gacgggaagg cgagcctgac cgacctcaag actatccagg actggcaatt gcggcgagag
     2761 ttccggcgcg tacccgggat catcgatgtg acggccttcg gcggaacgac gcaggagtat
     2821 cacgttgatg tcgatccggg aaaactcatc agctaccagg tcagccttgc gcaggtcatg
     2881 gaggccttga ccaacagcaa tgccaatgtc ggcgggaatt acctgaccgt gggcgcacaa
     2941 aattacaaca tccgcggact cgggctgatc aacgggctgg cggacatcga aaacgtgatg
     3001 gtcgccgaaa aagaagggac cccgattttc atcaagaccc tggggagcgt gtcggtcggc
     3061 catcgcgtgc gcctgggcaa ggtcggcatc gatgacaatg acgacgtggt ggaaggggtc
     3121 attctgctcc agcgagggta taaggcactc tcggtcctgg atcgcgttcg ggaaaaggtc
     3181 gaggagttga ataagtggaa gctgccggaa ggcgtcaaga tcaagacgtt ctatgatcgc
     3241 acggcactga tccatacgac ggtcgagacc gtcacggata ttctgatcag cggcatggtc
     3301 ctcgtgttta tcatcctctt cgtgttcctg gggcatttgc gcgcctctct cattgtcgcg
     3361 ctgaccattc cgatgtccct gctgttcacc tttacgatga tggtgatgat cgggcagtcc
     3421 gccaacttga tttccctggg cgccatcgat ttcgggatca tcgtggatgc gaccctggtc
     3481 atggtggaaa gcatcttttt ccatctggga catgatcgcc gcctgggtct gacggtgcca
     3541 cagcagatcg tgcgtgccgc ccgccaggtg gggcagccga ttttcttttc taccgccatc
     3601 atcgtggtgg ccttcattcc cctgttcacg atgaccggcg tgccgggaaa aatctttgcg
     3661 ccgatgtcga tcacctatgg attcgctttg ttcggcgcct tactcatggc ctttacgcta
     3721 gcccctgtcc tatgctcgtt gctcctgacc ggtgtggtga cggaacagga cacgcgtttc
     3781 gtcagcgcga ttcgccggac gtacctggcc gtcctgcgat gggcgctggc tcacaatatg
     3841 aaggtcatcg gtgctgcgtt gctgctgttg gtggcggcgt tcggtgccct gcagttcatc
     3901 ggcggggaat tcatgcccgc attggaggaa gggaacctct gggtgcgggc caccatgccg
     3961 gtggatattt cgttcgacga agcggctcga ttgaccggag aaatccggca gatgttcagg
     4021 aaatcgccgg aagtcgtcac gatcgtctcg caactcggca gacccgacga cggcaccgat
     4081 cccaccagtt ttttcaatgc ggaatttctg gccaatctca aaccacataa ggactggcgg
     4141 acgggactca ccaaagatca gctggtggag gaaatcgaag aacgcctcaa ggtcattccc
     4201 ggcatcattt ttaatttctc ccaggtgatt caggacaatg tggaagaagc gatgtccggc
     4261 gtaaaaggcg aaaattccat caagctgttc ggcaccgatc tgaaaacgat ggaagcgaag
     4321 gcgggagaaa tcgagtcggt gatgagaggc gttcacgggg tcaaagattt agggattttc
     4381 cgcctggtgg gccaacccaa tcttttgatc caagttgatc gggaagccag cgcgcggtat
     4441 ggcctgcgcg tgtccgacgt caacgccgtc gtgcaggcgg cggtgggggg gcaggccgtg
     4501 acgcaagtgt tcgaagggga gcggttgttc gatttagttg tccgctttct ccccgaattt
     4561 cggcgggatg aggaggccat cggtaatatt ctggtcaata cgccgaatgg cgcgcgcatt
     4621 cccctgaaac aggtggcgac gatcaagact cagaccggag ccttcatcat ctatcgggag
     4681 aacaatgaac ggtatattcc gatcaagttc agcgtgcgcg agcgcgacct ggaaagcacc
     4741 gtgaaagagg cgcaagcgaa aatggctcag gctgtctcgc ttcccgagcg ctaccggatc
     4801 gaatgggcgg gccaatacga tcaactcacg gcagagcaaa agcgcctcac gatgattgtg
     4861 ccgttgagcc tggtgatcat cctctttctg ctctatacca cattcaattc gctgaccaac
     4921 gccttgctcg tcctggtgac cgttcccttc gcgctgatcg gagggatttt ttcactggtg
     4981 ttcacgggaa cgcacttcag catctctgcg gccgtgggag tcatctcgac gctgggagta
     5041 gcgattctcg ggggagtcct gttaatctcc cgtattgagg attttcggcg cagcggcctg
     5101 gaattgcgtg aggccatttt gaagggcgcg gatgttcaga tgcgtccgat cctgatggcc
     5161 acgctggcgg cagccatcgg cttgcttccg gccgcactgg cgacggggat cggctcgcag
     5221 gcacagcaac ccctcgcgcg ggtcgtggtg ggaggaatgt tgactgcggc ggcgctgatt
     5281 ctcgtggtgt tgccggtctt gtatgaagtg gttcaccggc gcacggcgcc gcgaacaccg
     5341 ttggaagccg gagaggagcc accggcccct tattaacgac aacgtgtgcg agaggatgtg
     5401 atcatgatcg gagtcgggca gcagaggatg cgtcaagtgg tggtgctggg tctgattctt
     5461 cttggcctga tgggaatgcg cccagaggcg ggctgggctg gcagcatggt ctggccggtg
     5521 caaatgccgt atgaggcgcc gcccaagtcg gaaccggtgc ccgatgtgtc gatcgccccc
     5581 aatacgaagg ccctgacgcc ggaggaactc cagcgggcgg agtcgctctt gccgctcttg
     5641 gaaggaaaac aggaattttg ggccatggga gaatttgtcc atttgggcga gcccgcgtta
     5701 ccggtgctca ccaaggcgct cacaatgccc ggccctcgta tccggtacaa tgccattgag
     5761 acgatgtcca tgatcaaggc cccggctgcc acgcccgcct tactcgaagc ggcaaaattg
     5821 gcgacggaaa ttccccgtat ccgtgaacat gcgttgcggg tggccgtgcg gctcgatcct
     5881 cagcaggcac ctcccgccat cgaggctatg gccaaggatc ccaattcggt gatccgcaag
     5941 gcggcggcgt tcgaggcccg gtatgtgcgg aaaaaagaag tgattcaacc gttgatcgat
     6001 ctgctggggg atgaagaaaa gtttgtcgcg atgtccgccg tccaatcgtt atggacgttg
     6061 acccggcatg agacagagtt tcacgattgg gatgcctcca ataaacagga tcggcagaac
     6121 tgggcgcagg agtggatcga atggtggaat cagtcaaagg aaacctttga aattcctgat
     6181 ccgaagaccc gcaagcaggc cggctgacag aggaacgcac gaggcggttg cacagcctca
     6241 aaagcccgtg gtaggtacat acgcaggcaa tagcggcgtg accaaagtgg tcgcgcgtag
     6301 gcagataaag gtggatcatg gccatattgg caatcagtaa actggggaat ccgattcttc
     6361 gacagcaggc acagtcggtc tccccgagcg agataaaaaa ggcagccttt cagcagctca
     6421 tcgacgacat gtttgaaacc atgtatgacg aacccggcat cggcctggcc gccccgcagg
     6481 tcgggcgttc gcagcaactc gtggtcatgg attgtcccgg ggaagggggc ttccccaaga
     6541 cggtgctcat caacccgacg attcggttct acggccctga gcaggtggag ggctgggaag
     6601 gatgtctgag cgtggacggg ttgcggggca aggtgacccg gccgtcaacc gtgcgtgtga
     6661 ccggattgga tcgcaacggc aaaccgttcg attttgaagc cagcggactg tatgccgtgt
     6721 gtatccagca tgagctcgat catctcatcg gcaagttatt tatcgaccgc atgaccgatt
     6781 tctccacgct gacgcagatg gatgagtttc agaagtattg gcaaaaggaa ccggccagtg
     6841 tcatctgatc ccttccgggt cgccgggtcg ttgtgaactc gctcctacgc gttctgtcct
     6901 acctcaagcc gttccgaatg ctggcggtgg tgacctttgt gtgcgcgggg ctcgccacgg
     6961 cgttggaact ggtaccgccg tatctcgtca aaatcgtcat cgacgatgtc atccaagcca
     7021 agcggcctga gatgctcatg tgggtattag gcgccctgtt ggggtcgtat atcctgcgga
     7081 acctcatgag ctcgatgcgg gtacggttca acaatttact ggagcagaaa gcggtccata
     7141 cgctgcggat gcaggtattc ggggccttgc aacggttatc cctgagttac ttcgaaaacc
     7201 gctccaccgg cgagatcatg acccgtgtga ccagcgatac cgaacatgtc gaacgcatct
     7261 tcgtcgacgg gcttgaaggg ttgctgactg cctccctcac cctgatcggc attacgatca
     7321 tgatgttttt tttgaattgg aaattggcac tgctgtcgct cgtgcccatt ccgattctga
     7381 ttgtgtgtgc cgcctggttc acgaagcggg tgcacaaata ctacgccgag atccgaaaga
     7441 gtgtggcgga cctcaatgcc tacctgcaag acgccctgtc gggcatcaag gaaaccatcg
     7501 gcttcaatca acatgagtac gaacggaagc gttttgaaac cctgagccag acctacagcc
     7561 agaatagttt gcgggccatg tttctgtggt cgtggtattg gccgggcatg gtgttcgtcg
     7621 gcagtaccgg cacagtcctc attctctggt atggagcaga agaagtgatg gccggcaatt
     7681 tgacggtcgg ccaactggtc atgttcctgt cctatctcgg cttgttttat acgccgatca
     7741 acgagattca ttcgctgaac cacatgctgc aacatgccct ggcggccagc gagcgcgtgt
     7801 tcgaaattct cgacaccaaa gcggaggtcg aggatcggcc cggggccgta cggcctgtcg
     7861 aacggttgag aggcgccgtg gaatatcggc aggtagtatt cggctaccgg caagaaggca
     7921 cggtcttatc cgatgtgaat ctcaccgtca gacccgggga acgaattgcc ctggtaggtc
     7981 ccagcggcgc aggcaaaaca tccttgctga agctgctgat gcggttttac gatgtccgca
     8041 gcggggcggt gttggtggat ggttatgatg tccgggacct gccggtcgcg tttttgcggg
     8101 ggcagatcgg cctggtgcag caagagccct ttctctttaa cgggacggtg cgacagaata
     8161 ttgtgtacag cgacctctcg gccagccatg agcgcgtcga agcggcggcc cgcgcggcac
     8221 gggcccacga ctttatcacc cggctgccag atgggtatga tagctggatc ggcgagcggg
     8281 gcgtcaaatt gtcggtcgga cagaagcagc gggtctccat tgcccgtgtc ctgctgaagg
     8341 atccgccgat cattatcttc gatgaagcta cgtccaatat cgataccgag accgaggtca
     8401 aaatccgtga agcgctgaac gatctgacaa agggacgtac caccttcatt attgcgcacc
     8461 gcctcgcgac gttgcatgac gtagaccgaa ttatcgttgt ggagcggggc acgatcgtgg
     8521 aggagggcca ccacgatgcg ctcatgaagc ggggcggggt gtatgcggga ctctatgaag
     8581 cgcagttcaa gatttagcag gctgttgaga aaacccgcca gcggcgttct cggccgcacg
     8641 tctccttgcg acgtacccag agggtacgac tcagtcgccg ggcggcctgc ggcctggctg
     8701 gacggccttt ctgaacagcc tgcgacggcg gagaagaacg acgtattctg accagttacg
     8761 aaggagcgac aggaaaaaca tggtgctgat attcgaaagt gatccgctcg ataatgagga
     8821 tgcccggggg cggttttatc atgtgtgccg gggtcgaatt gatcggacgg aaattacctt
     8881 gcctcaaggg caatcgatat atgagtgtgc ggtatgcggg attgatctgg aaccggaaga
     8941 tttctggatc gcgaggcaaa agggatcggt gtgacggttg gtcgcaccga gtgacgatag
     9001 acagaggagt gcgttatggc tggaagtata ggccggaaga ctgtatcggt ttcacgcggg
     9061 ctgatgatgt tgtgcgagac ctgctacgat caggtgtatg ccgagaagct gcctgatgcc
     9121 aggaacttta tcgcggatca tgaggcgttg ttcgatacga tttgtatggg ttgcaccgac
     9181 atcaatcgtc cgctcatcga cgatatgctg gggagttcgg agtagnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnttgca ccgacatcaa tcgtccgctc atcgacgata
     9361 tgctggggag ttcggagtag cggtcttata tgctgaccga cagagcagtg ttaggcagca
     9421 tcaaccggtc aaatgggcct ctcgctaaaa aatgaactcg ttgcgacagc tagcgactca
     9481 tgtgcacgag gttattctga cactcccatc gggccggtag ctcgaattga gtcgtggttg
     9541 attcgctttc ccagtgcgaa ccctgatcgg ttgccgcttg gagttttaag aaagtcgtat
     9601 acggtgaagc cactcgtcgc gatcaacggc gcctcgatgt tcggtgagct cgcgattgta
     9661 tcctgcttgg aaaaggatgg ttggacggca ttttgggtgg atacatttca cggacgaaag
     9721 ttctggcgcg gcatgccgca tgtgacccag cctacggaac caccgaccaa ggtaaaagct
     9781 ctttatgctc gtattgctga tcgaaagggt ggcccttcag gttgttttga catcgtcgcg
     9841 tcgaaaggcg accgcatcat atggctagag tacaaaggcc caaaagatac ggcgaatcga
     9901 aacgagttgt gctggatcga ggcagctctt ggagcgggtg tcgctgagtc ttccctcttt
     9961 tttgtcggct cagatcggcg gccaaaagca gtagaacgta cgatctagcg gacattgcag
    10021 ctatatattc acgttggcta ctgcggaccg cggtcgaatg ttcggaggta gctccagacc
    10081 cgaagttatt ctacacgtac ctgctagtgt ccacgagcta cttacacgtc tttccgtcga
    10141 agtgcaggca tgttgattcg ccggatttca ccttccaggt tttcaggagc gggggctggc
    10201 cttcccgcgt ctccacgacg atatccacca atcctgattg cggtagtttc tcgatgtgag
    10261 ctttggcttc ctccggcacg tccacccaga atactgcgcc caaagtcgag atcagaatgc
    10321 ggctgttatc aacatccaca ttgatgatgg ggctgtagta gctcttttca gctgccgagg
    10381 acgtcccggg atgtgtgagg ccggtgaggc acaggatgag ggcgatcata acgagcaata
    10441 atcgcatcga atactccctt cgatgacaag gtcaaagccg gattatggca tcgttgctga
    10501 gcgattggta gggggaacct ggtcgaactg caggcgggga gaccgtcaca tgatatggat
    10561 atcggcgggc cggacttggc cgccggtgat ccaataggtg cgaatgtctg ctgcgggggc
    10621 atgcatgagt gaaaccagca gataggccgg aacaggcaag ttgatccgtt gccagatttc
    10681 ctcgtagtcc gtcgcgattt tgatgtcggt gtgagacggc cgcgccggat ccttcggatg
    10741 ggaatgatag accacttgca ggtccagacc gttcttgcga atgtcttttt tagccgcgga
    10801 aaagtcgccc atatccatca caaaggcaat ctccgcccgt tcttccggag agaggcgctc
    10861 cagatgcgcg accttgtctt catcgaacgt agaaagtttt tccgccccct cgacagccac
    10921 gatattcgtg atgcggtaaa ggtgcgtgac gactttgttc tttccggcta acagtccgca
    10981 acattcgaac ggagccagtt cgcgcgcatg cgcgacgatt tgatccagaa tgtgctgggg
    11041 gatcgacaga tcaggcatgt cagatggtgc aggccacgct gtagtctccg cccagatcct
    11101 tgatcgtcgg cgtcgcgctg cacaaagggc aacgcggatc tttcgggcga gagaccttgc
    11161 ggaatttcat ttccaaggca tcgtagatca ccagcttatc cgcgatcgtt tccccgatgc
    11221 cgagaatttc cttgatggct tctgatgcct gcagaatgcc catggtaccg gccagcacac
    11281 ccagcacgcc ggcttcctgg cagttcggaa ccaatccagc gggaggcggc tcgggataga
    11341 ggcagcgata acaggggaac ccggcatgag gcttaatgct cgtgagctgg ccttcaaagc
    11401 ggaacatgct ggcggagatc agcgtcttct tggcgaaaaa gcatgcatcg ttcaccagaa
    11461 accgcgtggt gaaattatcg gacccatcca agacgatgtc ataatccgac accaacccga
    11521 gaatattctc cgtatcgacg ttcagttgat aggtcttgat cgtaatgtcg ggattgatgg
    11581 ctgacagcat ccgtcggccc gattccacct tgggcacgcc gacggtcgcg gtggtatgca
    11641 ggatctgccg ctgaagattg gacaggtcga cgacatcgcc atcgaccaga ccgatcgtgc
    11701 cgatgcctgc cgccgccagg tacagggcgg cgggggaacc gagtccgccg gcaccgatca
    11761 atagcacctt ggcttttgcc agtttgacct gccctttgcc gcccacttcg ttgaggataa
    11821 tgtgccggct gtagcgctgt atttgttggt cggtaaattc cattcgactc gtctcttgtc
    11881 gttcgtcaga ggtgaatccg ccctctgagg tatggtctga aagaaggctg tagcctggtt
    11941 attccaccac gttcagctca atcgggtcga cgacgcagcc tttggtgcgg atgccgtcga
    12001 tggcccgttc gatctcttcg gtttcgccgg tgagttcgag gtccatccat ccggtggtct
    12061 cgcgcacatc ggcgcgtcgc acattggtga ccactctgaa ttcatgcccg atctgataga
    12121 tgatcggttc cttgatttta ttctcgggaa aacgaatgtg aaagcgtagg ctggtcatgg
    12181 ttatcctccg gcgatcgcgg gaacgatcga tacttcatcg ccgtctttca gcgaggtctc
    12241 tttcccgttc aggaagcgga tatcttcctc attgacgtag atattcacga agcgccgcaa
    12301 atcacctttt tcatcgcaca gccggttctt caggcccgga taggtggcat cgagcgatcc
    12361 gatcatgtcc acaatgttgg ctgccgcgga ttcaacctca ccctggccct tcgtgagggg
    12421 acgcaagggc gttggaatac gaaccttaat catgattcac caccactgtt ctttcccatt
    12481 ttgaatgttt tttggaagtt caccaggctc ggctggatcc ggaacggccg ccccacggca
    12541 tccacgaccg cttcctgagt cttgaggccg ttacccgtaa tgtacgcgac ggtgacgtcg
    12601 ttcttcttga tcagaccctg cttgacgagt ttgcacaaca cgccgatcgt caccccgcct
    12661 gccgtctcag cgaaaatgcc ttcggtctgc gccagtaact tgatcccttc taccacttct
    12721 tcgtccgtca ccgcgtccat tgcgcctttg ctttcggcgg tagccttcag tgcgtagtag
    12781 ccgtcagccg ggttgccgat ggcgagggat ttggcgatgg tctttggctt caccggcttg
    12841 aagaagtcgc ggccagcctt gaaagccgtg gcgatggggg aacacccttc ggcctgcgcg
    12901 ccgttgatct tggtacgcac ctcatcgacc aggcccagtg ccttcatttc gtgcaggccc
    12961 ttccagatct ttgtcagcag ggagcccgag gccatgggaa tcacagcctg atccggcgtc
    13021 cgccagccga gttgctccac cgtctcgaag gccaacgttt tcgacccttc ggcatagtag
    13081 ggacgaatat tgatgttgac gaaggcccag ccatgttcac cggcgatttc gctgcagagc
    13141 cggttgacat catcgtagtt gccctcgacc tcgaccacgt tcggcttata aatcaggttg
    13201 ccgagaacct tggcggcttc gaggtcggca gggatgaaga cataacactt catgcccgct
    13261 gcggcggcat gagccgcgac ggagttggcc agattgcctg tggaggcgca ggctaccgtc
    13321 tcaaaaccga gttcccgtgc gcgcgtgagg gcgaccgaga cgacccgatc tttgaatgac
    13381 agggtcggat gattgaccgt atcgttcttg atgtagagct cgtcgatgcc cagatgtgcg
    13441 ccgagattct tggcccgcac caagggtgtc agtcccgcat ggggcccgat gatcgaggtg
    13501 ctttcgaccg gtaacaggtc gatgtagcgc cacatgctgt tcgggccctg ctcgattttc
    13561 ttgcgcgaga tggtgctctt gatctcatcg tagttgtatt tcacttccag cgggccgaag
    13621 cacatctcac agacatggat ggctttgggt ggatattcct tcccacattc ccggcacacg
    13681 agcgctttca ttttcgacat ggagacacct tcgctacaag ttacgttgac ccggaagcgc
    13741 acatgtcggc ccaccgacgt cgccgtcttc aatcagctcc gtaatcgtgg gccgctcccc
    13801 gcatagcggg caatcggggt tccgcttgat gcggatttct ctgaactgcg tgcgtcgcgc
    13861 atcaaaatcc agcagccgat cggtcagcgg gcggccgatg cccaggatca gcttgagcgc
    13921 ttcagttgct tgaatcgttc cgataatacc ggccaacacg ccgatgacgc ctgcttcctg
    13981 gcaggaggcg acgagtcctt cgggcggtgg cttcttaaag acgcatcgat aacaggccga
    14041 ctttttcgga atgatggtgg tcacgcgccc gtcgaaccgg agaatgccgc cgtgcaccag
    14101 cggtttgccg gcgaaatagc aggcatcgtt gatgagaaac ttcgtcggga aattatcgac
    14161 gccgtcgatg accacatcgt aaccgctcat gatcttgagg gcgttgcctg ccgtgagccg
    14221 ctcttcgtac atcaccacgt tcacgtcggg attcagcgct tggatcttct ctttgccgga
    14281 gacgactttg gggcgcccga cgtccggtgt ctggtgaatg acctgacgct gtagattgct
    14341 gagatccacc acatcgctat cgatcagccc gatcgtgccg atgccggcgg cagccagata
    14401 taaggctgcc ggcgaaccaa gaccaccagc cccgacgatg agaaccttgc cctggatgat
    14461 cttcttttgg cccttgccgc cgacttcagg caacaggatg tggcggctgt agcggttgat
    14521 ttgtgtttct gtaaattcca tgtccgacgg aagctgaaaa ccgtcaccag cggcgttctc
    14581 gcatcattca ggccctcaac gtaccgcaag ggtacgcctc ggcccttcat tcgctgcggc
    14641 cttgctggat gacgcttttg agcctcctct ccctaaatgg tctgccgcac gctcaaatat
    14701 tgaaatactt ttctatactg atataccgct cgccggtgtc gcaaagaatc gtgacgacat
    14761 ttttgccggg acccaattcc tcagcgatct tctgggcggc gaagacgttg gcgccggcgg
    14821 aaataccgac cagcagccct tctttcttgg cgagcaattt ggccgtttga taggcttcat
    14881 cgtcggtcac agtaatcacg cggtccagca aggtacggtt caacactttg ggcacgaacc
    14941 ccgcaccgat cccttgaatc ttatgcggtc ccggatctcc acccgacagc accggtgagc
    15001 cggccggttc caccgccacg attttcgccg ccggattccg ctcccggatc acttctccgc
    15061 aaccggtgat cgttccgccg gtcccgacgg ccgccacgaa cgcatcgacc cgaccatcca
    15121 gcgcctccac aatttccgga ccggtggttt tacgatgcat ggctggattc gcgggattgg
    15181 agaactggtc gggcatatag tacgacggat tctgcgcgac gatgctttcc gcttccttga
    15241 tagagccctt catcccttcc caggccgccg tgagaaccag ttgtgcgcca taggaggaca
    15301 acaggctcgc acgttccatg ctcatgcttt ccggcatgac taaaatcaat ttatacccac
    15361 gaaccgcggc caccaatgcg aggccgatgc cggtgttgcc gctcgtcggt tcgacaatgg
    15421 tcccgcctgg cttcagtttg ccctgccgct ccgcctcgtt gatcatgttg aggcaaatgc
    15481 gatccttgat gctcccgccc gggttgaacg attcgacttt cgcgtagatc gtcgccgagc
    15541 ctggtttggt caaccgattg agccgcacca gcggggtgcc tccgatcaac tcggtgatgt
    15601 ctccgtgggc cttcgcggtc accggccacc gcccatgtag aagagaaatt ccacatgatc
    15661 gccatctttc agctgagtgg tctcgagatg tgtccgttcc agcatggtgt cgttcacttc
    15721 aaccgcaacc atttgcggtt cgatgctctt tgctttcagc aggtcgagga ccgtgccgcc
    15781 tgcgatctct tccgctttgc cgttgatcat cacctgcata tctgctcctg agatggtaag
    15841 agtctgacag gatactgaac atgtccgcca gcggttctcg catctctcag gtcctcaacg
    15901 taccgcgagg gtacgcttcg tcccttcgtt tgctgcggcc ttgctggaag gccatgttga
    15961 gtatcctgtc gaaagggatt ctggttgcgc cttacacgcc gttgctcggt gttcaatgag
    16021 ctcgacgcga tttgcagcaa actgctaaag aatttcaaga agttagcaaa cgcgttcttg
    16081 tgttgtcaag gcaacggacc cccaacgggt gcgacgagga agttgctgcg aagtcgtctg
    16141 gtagagacgg ccgctttctt gactttcacc atgcctagct tacagtatgc gccggtctg
//
LOCUS       DAOQDS010000079        16152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1501_length_16152_cov_19.319811, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000079 DAOQDS010000000
VERSION     DAOQDS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16152
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1501_length_16152_cov_19.319811"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..>2997
                     /locus_tag="PK589_15720"
     CDS             14..>2997
                     /locus_tag="PK589_15720"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ21108.1"
                     /translation="MVRHPPVTIPAGDTLLDVLRWRAEHQPDQTAYVYLREGLSADSV
                     LTYRDLLTRARSIASYLQARFAPGDRLLLVYPPGLEFVQAFWGVLYAGLIAVPVPPPD
                     AFRLKAGVARVQRLAEDAGAAGALTTAQMLETLRSQELVASLHQWVSSDQALSADSSG
                     WTDLQQLPTHLAYLQYTSGSTSAPKGVMVSHGNMTAQSRCITDAGGYDAGSVTLSWMP
                     HFHDYGLVKGIIQPAWIGRPSYLMSPLTFLKRPLRWLEAIQRYAVTHSGAPNFAYRRC
                     VEATTPEERAGLDLSQWQVASCGAEPIAPDTIQRFAAAFAPSGFQREAFFPAYGMAEY
                     TLLISLKRRGAAPTVTTLDAAALEKGAVIEAVADDKPVRKVVGCGMPVGDTRVVIAHP
                     ETRCRCAVQQIGEIWLSGASTTQGYWNKPDETSHTFGASLADTGDGPFLRTGDLGFVK
                     DGEVFVTGRFKDLLIVRGRNHYPQDLERTVESCHGALRAGGAAAFTVEEAGEETVVLV
                     QEVERQASPCSTEDLAAAMRAALSEQHDLHASTIVFIKAGSLPKTSSGKVQRRACREQ
                     YLAGRLSIIGTSVAPHPVAQSSPAPIHTDAWQRLSGEARTSAILQTVQKLLADRLGCM
                     PQAIAVDRPIHLLGLDSLMAADVLHRLEESFHLSLSLSALLGGATPADLTTMIERGLA
                     GESRAQSVAAIADPAIATVFPLSENQAALWFLSQLDPDSAAANVSVLLPLPSDLKADR
                     LRAALEAIGDQQAMLRTTFETQEDGPVQRIHDRMPLSWSEIDSSTWEWPQLRGESVKA
                     AAVPFNLTQGPLWRATLFHGGQQSWLLLVAHHIVVDGWSMMQLVDDLKRRCEVAGSCS
                     SEYSPEPQDRPAPYREFVEWHRAILEGEEGSRLGQYWKAKLAGELPNYDMLYDRPEIT
                     VEPSHYAWHAFHIDEDLTRRLKKFARTEGTTLYAVCLSALQILLTRYTAQEDTAIVTP
                     VFGRSRARFARTVGDFVNMLVLR"
     assembly_gap    2998..3097
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3098..>7554
                     /locus_tag="PK589_15725"
     CDS             <3098..>7554
                     /locus_tag="PK589_15725"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNJ21109.1"
                     /translation="EPGVVVGLCLERSLNMIVGLLGILKAGGAYLPLDADYPTERLEY
                     MLRDSQVRVLVTQQDQLVRLPATNPHTICLDSEWAQIAKCPDSPIDVTDALDNLAYVI
                     YTSGSTGHPKGVMIEHRSIANYVRAITKIVDLTGEDRVLQFASLSFDTAAEEIFPCLA
                     AGATLVLRTATMVDSVSGFLDRCRELTVTILDLPTAYWHELVTRMALEQVAFPSSIRT
                     VIIGGERVLPQIVQRWTDLIGATVRLINTYGPTETTVAVTWSDLTGLEAHDELIGDLP
                     IGRPIPHTSVFVLDRNQQPVPVGVAGELYIGGVGVARGYRGRPDLTETQFVPDPFSKQ
                     PGARLYRTGDLVRWRPDGQLDYRGRADRQVKIRGYRIELEEIEAVLNRHQDLERAVVE
                     VREDQPGDKRIVAFMVPRPNNRLGLVQLREQLRSQLPAHMIPSTFIEMETLPLTVNGK
                     VDRKALQVAADSRASKVELTSQYLAPRTPTEQVLADIWGEILQLKDVGVHDHFFELGG
                     HSLLATQLVSRVQSLFRITLPLRQIFERPTIATLAEVITQAQAGANSHQGESGYVITR
                     ARRGIPLPLSFAQERMWFLYRLSPDAVAYNIPASVRLHGPLNKPALRWAVAELVRRHD
                     ALRTTFAHVDGQPRQIIQDVLDPTWTEEDLRGVPAELREPRALELATAEARRPFDLEQ
                     GPLLRVFLIQLGDEDQIVILNTHHIISDQWSYGIIARELVTSYNAFCAGRPFAVQPDL
                     AIQYADFAQWQRGWLTGPVLEEQLAHWKTKLVDLPVLTLPTDRPRLPVHSFKGDHVSL
                     DLSWSLINRLKQLSVREGVTLYMVFLAGFFGLLHRLTQQKDLVIGTPIANRNRLEIEE
                     LIGTFVNTLVLRADVTGELTFRDLLRKVRDLSLDAYAHQDIPFEKLVEELRPDRSQGG
                     LPLVQVLFNFANTPFARTEFHQLSWTPYEVSRGAAQLDLGLSIDPYASRKAYLEFNTD
                     LFDRASAERWISQYRQLMETVAEHADAELGRVSILTGQEQHRILREWNATDRPVDQGL
                     CFYQLFEAQVSRTPDAVAVRFEGREWSYAELNRRANRLAHRLRECGVGPDAVVPVFLE
                     RSPDLLISLLAVMKAGGAYLPLVPGLPIRRLAAMIEASHAAVLITESALLGGLPQHQL
                     PVLCLDQEAEVLARQAEENPSPRSGPEHLVYVLFTSGSTGQPKGVEIEHRALVNFLRS
                     MQQEPGITHRDVLVALTPLSFDIAGLELYLPLLVGATIILANRQQAMDGAWLQRELDH
                     GAVTMLQATPATWRMILQFGWKGGRGVKVLCGGEALPRELAQELLARAGSVWNVYGPT
                     ETTIWSTLERVRNAERTISLGRPIANTQVYVLDGNLEPVPVGIPGELYIGGMGLARGY
                     RGQSQLTAERFITNPFKAGERLYRTGDQVKWLPDGRLDYVGRIDHQVKLRGFRIELGE
                     IESVLGNDATVKQAVVIVREDSPGDKRLVAYVAPREGSACDPSSLRRALR"
     assembly_gap    7555..7654
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7655..8593
                     /locus_tag="PK589_15730"
     CDS             <7655..8593
                     /locus_tag="PK589_15730"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HNJ21110.1"
                     /translation="LFQAPTIEQLADVLADEGCTVRWRSLVAIQPEGKRPPLFAVPGV
                     GGNVLVFARLAKLLGDDQPFYGLQARGLDGKEKPFMRVEEMAAHYIEEIRSVQPHGPY
                     FIGGTCTGGLAAYEVAQQFTAQGEEVILAVMESWHPRSYLNHWSRPPYLLWPLLFVWM
                     KVTTYLRLMRRLPADEWASFWKGKLKRMWNLMHHTEAAEHQDEFLYKDQVTYATFHAV
                     ARYELKPFRGQVLNVIASKHPLTNSSDDTRLVFGEWAMGMSRTVYLPAEDSGRLFVAP
                     HVQELARQLKAFWQEAEAVYRKRPDGQGDGPTTKAA"
     gene            8647..>10196
                     /locus_tag="PK589_15735"
     CDS             8647..>10196
                     /locus_tag="PK589_15735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HNJ21111.1"
                     /translation="MMKLYRFLLFLLRDARTMMILMVMTGLLAGLSSVGLLAVINKLI
                     NGPGTASELFAVAFIGLAVLKVGSNYLSQLLLVTFAQKTILKLGMDLCWKVVRAPYRT
                     LERRGPHEILATLTDDTNALAWAVNGLPGLAINVAILGGCSLYLAWLSWQAFLGVVIL
                     AVLGLLGYRQLYNRVLQSSLAVRDSKGVLFEHFRSLTEGMKELMLHRGRRETFVEEDI
                     RQAADALRRHNLTTTKQYLVTDSWTQVLFYGLIGVILLLFPRVLSLSGESLTGYAFAM
                     LYMIGPMWGLLGMVPTLSRGQVALEKIESLGLALDEGGREGGAERPMAQGRPCVEFSN
                     AVFAYESKGEDERSFRLGSLDLSIRAGELVFIIGGNGSGKSTFVKVLTGLYLPQQGQV
                     TLNGEAITPATQDWYRQHFAAVFSDFYLFKKLLGLDPSLVASQADGWLKTLRIDHKVR
                     IEQGEYSTVNLSQGQRKRLALVTAMLEDRPFYVFDEWAADQDPQYKDVFYGELLPNLR
                     ARGKGVIVV"
     assembly_gap    10197..10296
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10297..10925
                     /locus_tag="PK589_15740"
     CDS             <10297..10925
                     /locus_tag="PK589_15740"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNJ21112.1"
                     /translation="ERTRAARFAFERDRQRFVLSHGVLRQLLARYTHIKAGEIQFITG
                     RHGKPALAAPSVPSGNIQFSLSHSGEYALVAVARGREVGVDVEVFKANMDCAKLAERF
                     FAPREAQAMATLCGEPQQRMFYRLWTAKEAYFKGLGVGLSLGLERCEIVFDGESPHAR
                     VRLADSGTSDHAWQIQSLSLPDGLAGAVAASGGDWELHRYESTAYSLA"
     gene            complement(10922..11467)
                     /gene="tadA"
                     /locus_tag="PK589_15745"
     CDS             complement(10922..11467)
                     /gene="tadA"
                     /locus_tag="PK589_15745"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNJ21113.1"
                     /translation="MPIVRTRCVLSLTGITHDTMSLPDDLDRQFMQEALSLAREAPLI
                     GEVPIAALLVRDGVVIARAHNLRETVQDPTAHAELLVIREAAKQMNSWRLTESTLYVT
                     LEPCCMCIGAIVLARIPRLVFGAFDPKAGACGSIMDIPPEPRLNHRVDVVGGLCAEDS
                     QALLQDFFRGLRRDAAQRKSG"
     gene            complement(11521..12537)
                     /locus_tag="PK589_15750"
     CDS             complement(11521..12537)
                     /locus_tag="PK589_15750"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNJ21114.1"
                     /translation="MTHEELEEAVPLYAIGALERSERQAIEAHLLSGCAACHAALKDY
                     QTVASLLPFGLTPATPPNTLKAKILMAPASTTSQPETEQPGPRSSLEPGEWMNHLFPP
                     ITPARSLPFRIAMGFAAFVVVVGGGYLAWMNYTQTAQRSGEIQQLQAAVQRETSRASS
                     LQSELRQREQTLDSLKTDLDQRTTEVAELRDQLLQREAELDDAHQQLTQHDSSLQRLA
                     QQSETFVGLFKNPTSKVVLLSGSDMAKSAGAFLLIDPASKKAWLYAFNLPALPNGKVY
                     QLWAIDDKPVSAGVFGLDAGQKARMLIKNMGEFPRMKKFAVTVEPDGGRPEPSGAIYL
                     IGQI"
     gene            complement(12534..13139)
                     /locus_tag="PK589_15755"
     CDS             complement(12534..13139)
                     /locus_tag="PK589_15755"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ21115.1"
                     /translation="MESAPRQAASTIEPKLLARVAKGDQAAFGQLYDQSSTLLFTLAY
                     RILNDRDEATELLQDVYLEVWRKIAKYDVGRGSPIAWLVTLTRSRAIDRLRSRASRGQ
                     HVVADSFEHPLVSVTPDVSPNPYEAREDTEMRQMMAKAILDLPLPQQQAIEMAFYQGL
                     THTEIAAKLNQPLGTVKTRIKLAMSKLRASLQETLPQGHQL"
     assembly_gap    13232..13331
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13332..13786)
                     /locus_tag="PK589_15760"
     CDS             complement(<13332..13786)
                     /locus_tag="PK589_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21116.1"
                     /translation="MPLIWCSISGHGYGHAAQVVPVLNALARLVPDLKAILRTSVPSG
                     FFEPRLTVPWQLSQAQQDIGCIQNGPLSIDVAGTWAAHHALHADWEKKIEAEAMLIRQ
                     AGPSLVLSDVSHLGIAAGAAAGIPTIGLCSLSWDLVLEPFVDPAEPDHAV"
     gene            complement(13945..14145)
                     /locus_tag="PK589_15765"
     CDS             complement(13945..14145)
                     /locus_tag="PK589_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21117.1"
                     /translation="MTLDAARQRIESAITQYGQHAAMAIELVINEVRSDMGRDAVNEL
                     IDEFDLELMYNIAPMESDYSNS"
     gene            complement(14163..14480)
                     /locus_tag="PK589_15770"
     CDS             complement(14163..14480)
                     /locus_tag="PK589_15770"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ21118.1"
                     /translation="MDISAFRQMVEKNPKGFLGRYGLGNKILQEGGSLEEAAEHLTVA
                     TQLDPTHVASHLALGRALVKLGKKEDAKPVLKAGIDAAVSGRSNGGVDLVPELQQLLR
                     TLG"
     gene            complement(14546..15313)
                     /locus_tag="PK589_15775"
     CDS             complement(14546..15313)
                     /locus_tag="PK589_15775"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ21119.1"
                     /translation="MAENDKHRARIFLHRGDLVQARAAWEAAVKDDRLTGTPRELSNS
                     LGNLGNTLALAGAFEEADACYKEVLAIQRNERDPHAIAHTLVNLGNLHIGADKPEKAR
                     PYYLEALDLLKPLNDHRALGILYHNLALEEARQQQWDESVRLFTQALDSHRVVGNEEG
                     LAGTYSQMGKMFLDSGRSVEAERCFNNATEHFIKLGNPSGEAAVLRELADLYEQRQDT
                     IAAIRCVERLHHLALGTGRTPSTADRDRLTRLRAAHS"
     gene            complement(15399..15821)
                     /locus_tag="PK589_15780"
     CDS             complement(15399..15821)
                     /locus_tag="PK589_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21120.1"
                     /translation="MQFDAALAAQETLQEAQTELGVDWDQATELEETFSSNAGSTARD
                     AYEQLLALAARHPKAHRFQAFCIYITWQQVTEETIARHFQTGMTLAESYLASPDGKDP
                     RHLAHVAELLDSFRAGLGLDEEDDIVVEFRKDTPKGGD"
BASE COUNT         2986 a         4435 c         4926 g         3405 t
ORIGIN      
        1 ggttgagcat tctgtggtca gacatccacc ggtcaccatt ccagccggcg acacccttct
       61 cgatgtgctg cgttggcggg ctgagcatca gcctgaccag acggcctacg tctatctccg
      121 tgaaggactg agcgctgaca gcgtactgac gtatcgagac ctgctgacgc gcgctcgttc
      181 gatcgccagc taccttcagg cccgattcgc gccaggggac cgactcctcc tggtatatcc
      241 gccggggctt gaattcgtac aagccttttg gggcgttctc tatgccggcc tcatcgctgt
      301 gccggttcct cctccggatg cgttccgcct gaaggccggc gtggcgcgcg ttcaacgcct
      361 cgctgaagat gccggcgccg cgggagcgtt gaccacagct cagatgctgg agacactcag
      421 gtcgcaggag cttgtcgcgt cgttacacca gtgggtttcg agcgaccagg cgctctcagc
      481 agattcttcc ggttggactg acctacagca actgcccaca cacctcgcat atttgcaata
      541 tacgtccggc tcgacctcgg caccgaaagg ggtgatggtc agccatggga atatgaccgc
      601 gcagagccgg tgtattaccg atgcgggtgg ttacgatgcc ggatccgtga ctctgtcctg
      661 gatgccccat tttcatgatt acggattggt gaagggcatc attcaacctg cgtggatcgg
      721 gcgtccgtcg tatctcatgt cgccgctcac gttcctcaaa cgccccttgc gatggctcga
      781 agctattcaa cgctatgcgg tcacgcatag cggcgcgcca aactttgcct atcgccgttg
      841 cgtggaggcc acaacgccag aggaacgggc aggattggat cttagccagt ggcaggtggc
      901 gagttgcggg gcggagccta tcgcaccgga cacgatccag cggtttgccg cagcgtttgc
      961 gccatccgga ttccagcgcg aagccttctt tcccgcctac ggaatggcgg aatacacgtt
     1021 gctgatttcg ctcaagcgaa gaggagcggc gcctaccgtg accacgctgg atgccgcagc
     1081 gttggaaaag ggtgccgtca tcgaagctgt ggcggacgac aagccagttc gaaaggtcgt
     1141 gggatgcggc atgccggtcg gcgacactcg cgtggtgatt gcccatcctg agacgcggtg
     1201 ccgttgcgcg gtgcaacaga tcggtgaaat ctggctgtcc ggcgccagca cgacacaagg
     1261 ctattggaac aagcctgacg aaacgtcaca cacgttcggc gccagcctgg cggataccgg
     1321 agacgggccg ttcctccgta ccggcgacct cggatttgtg aaggatggcg aagtcttcgt
     1381 gacggggcgg ttcaaagatc tgctgatcgt tcgaggccgc aaccattatc cccaggatct
     1441 cgagcgaaca gtcgagtcat gccatggcgc gctccgggcg ggaggcgcgg cagcgtttac
     1501 tgttgaggag gccggcgaag agaccgtggt gctggtccag gaggtggagc gccaggcgtc
     1561 gccttgttcg accgaggact tggccgcggc catgcgtgca gccctgtccg agcagcacga
     1621 cctccatgcg tcgaccatcg tcttcatcaa ggccggcagc cttccgaaga cttccagtgg
     1681 gaaggtgcaa cggcgcgcct gccgtgagca gtacctggca ggtcgattgt cgatcatcgg
     1741 aaccagtgtg gcgccgcatc cggtggcaca atcatctccc gcacccattc acacggacgc
     1801 gtggcagcgg ctttcagggg aggcgcgcac aagcgccatt ctgcaaacag tgcagaagct
     1861 attggctgat cggctcggtt gcatgccgca ggccattgcg gttgatcggc cgattcatct
     1921 cctgggactc gattcgttga tggcagcaga tgtgctgcat cgcctggaag aatcgtttca
     1981 tctgtcgctg tcgctgtccg cgcttctggg cggtgcgaca cccgccgatc tgacgacgat
     2041 gattgaacgg gggctggccg gtgaatcgcg agcccagtcc gttgctgcta ttgcagatcc
     2101 cgctatcgcg acggtgtttc cactctcgga aaaccaggcc gccctatggt ttttgagtca
     2161 actggatccc gacagtgccg cggcgaatgt gtcggtgttg ttgcctctgc cgtccgacct
     2221 taaggcagac cggttgcgcg cggcccttga agccatcggc gaccagcagg ccatgctgcg
     2281 caccaccttc gaaacgcagg aagatggacc ggttcaacga atccatgacc ggatgccgtt
     2341 gagttggagt gaaatcgaca gctccacttg ggagtggccg cagttacggg gcgagagtgt
     2401 aaaggctgca gccgtcccct tcaacctgac gcagggcccc ctgtggcgcg cgactctttt
     2461 tcatggcgga cagcagagct ggttgttgtt ggtcgcccac catatcgttg tggatggctg
     2521 gtcaatgatg cagttggtcg atgatttgaa gcggcgctgt gaagtcgccg ggtcatgttc
     2581 atccgagtat tcgccggaac ctcaagaccg tcccgcacca taccgggaat tcgtcgagtg
     2641 gcatcgggcg atcctggaag gggaagaggg gagccgcctg ggccaatact ggaaagcgaa
     2701 gctggccggt gagttgccga attatgacat gctgtacgac cggcctgaga taacggtcga
     2761 gccgagccac tatgcctggc atgccttcca catcgacgag gacctgactc gccgcctcaa
     2821 gaagtttgcg cggaccgagg gcacgacctt gtatgccgtc tgcctgagcg cgctgcagat
     2881 cctgctcact cgctacaccg ctcaagaaga cacggcgatt gtcacgccgg tgttcggccg
     2941 tagccgggct cgattcgccc ggacggttgg tgactttgtg aacatgttgg tgttgcgnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa cccggtgtcg tcgttggcct
     3121 ctgtctcgag cgctcgctca acatgattgt ggggctgctc ggcattctga aggcgggcgg
     3181 cgcttacctc ccgttggatg ccgattatcc gaccgaacgg ctggaataca tgttgcggga
     3241 cagtcaggtg cgggtgttgg tgacacaaca ggatcaactc gtgcggctgc ccgccacgaa
     3301 tccgcatacg atctgtctcg attcggaatg ggcgcagatt gcgaagtgtc ccgacagccc
     3361 tatcgacgtg accgatgcgt tggacaatct ggcctatgtg atctacacgt cggggtccac
     3421 gggtcatccg aaaggggtga tgatcgagca tcgctcgatc gccaactatg tgcgagccat
     3481 cacgaagatc gtcgacctga caggagaaga tcgagtacta caatttgcct cgttgagttt
     3541 cgatactgcc gccgaagaga ttttcccctg tctggccgcg ggggcgacat tagtgctgcg
     3601 taccgcgacg atggtcgatt cggtatccgg atttctggat cgctgccgtg aattaaccgt
     3661 gacgatcctg gatctgccca cggcctactg gcatgagctg gttacccgga tggcgctgga
     3721 acaggtcgcg tttccttctt ccatcagaac ggtgatcatc ggtggcgagc gagtgctgcc
     3781 gcagatcgtg cagcgctgga cggacttgat cggggcgacc gtccgattga tcaataccta
     3841 tgggccaaca gaaacgaccg ttgcggtcac ttggtcggat ctgactggtc tggaggcgca
     3901 tgatgaattg atcggcgatc tgcccatcgg tcgccccatt ccgcatacgt ccgtcttcgt
     3961 attggatcgg aatcaacagc cggttccagt gggtgtggcg ggggagctct acatcggtgg
     4021 cgtcggagtc gcgcgcggct atcggggcag gccggatctt acggagactc aattcgttcc
     4081 cgatccattt tccaagcagc cgggagcccg actgtatcgt acaggcgacc tggtgcgatg
     4141 gcgacccgac gggcaattgg attatcgggg ccgagccgat cgccaggtca agattcgcgg
     4201 ctatcgcatc gagttggaag aaatcgaggc cgtcctgaac cggcatcagg atctggaacg
     4261 ggcggtggtc gaggtacggg aggaccagcc aggagacaag cggatcgtgg cgttcatggt
     4321 gccacgtccg aacaaccggc ttggattggt gcagttgcgc gaacagcttc gcagccaatt
     4381 gccagcgcac atgattccct cgaccttcat cgagatggaa acgcttccgc tgacggtgaa
     4441 cggcaaggtg gatcgaaagg cgctgcaagt tgccgcggac agtcgcgcca gcaaggtcga
     4501 gctgacctct cagtacctgg cgccacgtac tcccacggaa caggtcctgg ccgatatctg
     4561 gggggagatt cttcagctca aagacgtcgg cgtgcatgat cacttctttg aactgggtgg
     4621 gcattcgctc ctcgccacac aactggtatc cagagtccaa tctctgttcc gcatcacgct
     4681 cccgctccgg cagattttcg aacgtccgac gattgccacc ttggccgagg tcatcacaca
     4741 ggcacaggcc ggagccaatt cccatcaggg ggagagtggg tacgtgatca cgcgagccag
     4801 aagaggaatc ccgcttccgc tgtcgttcgc gcaggagcgg atgtggttcc tctatcgact
     4861 ctcgccggat gccgtggcct ataacatccc ggccagcgtc cggttgcatg gtccgctcaa
     4921 caagccggcc ttgcgatggg cggttgctga actggtccgg cgccatgacg cgctcaggac
     4981 cacctttgcg cacgtggacg gtcagccacg tcaaatcatt caggacgtat tggacccgac
     5041 ttggacggag gaggatctgc gcggagtgcc ggctgagctg cgcgaaccac gggctctgga
     5101 acttgcgacc gcggaggcgc gacgaccgtt tgatctcgaa cagggaccgc ttctgcgcgt
     5161 gtttctgatc cagcttggtg atgaagatca gatcgtgatc ctcaatacgc atcacatcat
     5221 ctccgatcaa tggtcttatg gaatcatcgc gcgggaactc gtcacgtcct acaatgcctt
     5281 ctgcgcgggc agaccgtttg ccgttcaacc ggatctcgct attcagtatg cggacttcgc
     5341 gcagtggcaa cggggatggc tcaccggtcc tgtgctggaa gaacaactcg cgcattggaa
     5401 gaccaaactt gtcgatctgc ctgtcctgac cttgccgaca gaccggccgc gcttgccggt
     5461 gcattccttc aaaggcgatc atgtgtcgct cgacttgtcc tggtcgctga tcaatcgtct
     5521 caagcagctg agtgtgcgcg aaggcgttac cctgtacatg gtattcctgg ccggattctt
     5581 cgggctcctc caccgcctca cacaacagaa ggacctggtc attggcaccc cgatcgctaa
     5641 ccgtaaccgt ctcgagatcg aggagctgat cggaaccttc gtcaatacgc tggtgttgcg
     5701 agcggacgtg acaggtgagc tgacgtttcg tgacctgctg cgaaaggtgc gggacctgtc
     5761 gctcgacgcc tatgcgcacc aggacattcc ttttgagaag ctcgtggaag aattgcggcc
     5821 ggaccgtagt cagggaggct tgccgctcgt ccaagtgttg ttcaactttg ccaatacgcc
     5881 ctttgcccgc accgagtttc atcaactctc ctggactccc tatgaagtga gccgtggcgc
     5941 ggctcaactt gatctcggcc tttctatcga tccctacgcg tcgcgaaaag cctatttaga
     6001 attcaatacg gatctgttcg atcgcgcctc cgccgagcgg tggatttcgc aataccggca
     6061 gctgatggaa acggttgccg agcatgccga tgcagaactc gggcgcgtgt cgattctcac
     6121 cgggcaggag cagcatcgaa tcttgcgcga atggaatgcc acggatcgac cggtagatca
     6181 gggcctgtgt ttttatcaac tgttcgaggc gcaggtcagc cggacgccgg atgccgtggc
     6241 cgttcgcttt gaagggcggg aatggtccta tgcggaactg aatcgtcgcg ccaaccggtt
     6301 ggcgcatcgt ttgcgtgagt gtggcgtagg gccggatgcg gtcgtccccg ttttcctgga
     6361 gcgctcgccg gatctgctga tcagtctcct ggccgtgatg aaagcagggg gtgcctacct
     6421 gcctttggtt ccggggttgc cgatacgaag gctggcggcg atgatcgagg ccagtcacgc
     6481 ggcagtcctg atcactgagt ccgccttgct cggcgggctg ccgcagcatc agcttcctgt
     6541 gctgtgtctg gatcaggagg cggaggtcct ggcgcgtcaa gcggaggaga atccgtcgcc
     6601 gcgatccggt ccggagcatc tcgtgtacgt gttgttcacc tccgggtcca ccggccagcc
     6661 gaagggggtc gaaattgagc atcgcgcact ggtgaatttt ctccgttcca tgcagcagga
     6721 accggggatc actcatcgcg atgtcctggt cgcactgacc ccgctgtcgt tcgatatcgc
     6781 ggggctggaa ctctaccttc cgctcctggt gggcgccaca atcatactgg ccaatcggca
     6841 gcaggcgatg gacggggcct ggttgcaacg ggagctcgat catggcgcgg tgaccatgct
     6901 gcaagcgacc cctgccacct ggcgaatgat tttgcagttc gggtggaagg gtggacgcgg
     6961 agtgaaagtc ttgtgtggag gagaggcact gccgcgcgaa ttggcgcagg aactgctcgc
     7021 cagggccgga tccgtatgga acgtctatgg gccgaccgaa acgacgattt ggtcaacatt
     7081 ggaacgcgtg cgtaacgctg aacggacgat ttctctcggc cgtccgatcg cgaacacgca
     7141 ggtctacgta ttggatggca atctcgaacc ggtccccgtc gggattccag gtgagttgta
     7201 tatcggcggg atgggtctcg ccagaggcta ccgaggccag tctcaattga cggcggagcg
     7261 ttttatcacc aacccgttca aggctggtga gcgactctat cgcaccggcg atcaggtgaa
     7321 atggttgccc gatggacgac tggattatgt cggtcgaatc gaccaccagg tgaaattgcg
     7381 cgggttccgg atcgagctgg gtgaaattga atcagtgttg gggaatgatg cgaccgtgaa
     7441 acaggcggtc gtgatcgtgc gggaagattc gccgggcgat aaacgtcttg tggcctatgt
     7501 ggcgccgcga gagggtagtg cctgtgaccc atccagcctt cgccgggcac tgcgnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttgttc caggcgccga cgatcgaaca
     7681 actggcggat gtcctggccg acgaaggttg cacggtacgg tggcgctccc tcgtggccat
     7741 tcagccggaa ggaaagcggc caccactatt tgccgtgccc ggggttggag gtaatgtact
     7801 ggtgttcgcg cggctcgcca aattattggg agacgatcaa cctttctacg gattgcaggc
     7861 tcgcggcctc gacggcaaag aaaagccgtt catgcgggtg gaagagatgg cggcccatta
     7921 cattgaggag atccggtcgg tgcagccgca cggtccctat ttcatcggag gtacctgcac
     7981 cggcggcctg gccgcctatg aagtcgcaca acagttcacg gcacagggag aagaagtcat
     8041 cctggctgtc atggaatcct ggcatccacg atcctacctg aaccactgga gtcgtccgcc
     8101 ctatctgctc tggccgttgt tgttcgtgtg gatgaaggtc acgacctacc ttcggctgat
     8161 gcgccggttg cctgccgatg aatgggcgtc gttctggaag ggaaagttga aacgcatgtg
     8221 gaatctcatg caccataccg aagccgccga gcatcaggac gagttcttat acaaagatca
     8281 agtcacatac gcgaccttcc atgccgtggc gcggtacgag ctgaagccct tccgtgggca
     8341 ggtgttgaat gtgatcgcat ccaagcaccc cctcacgaac tcgagtgatg atacccggtt
     8401 ggtgttcggc gaatgggcga tgggcatgag ccggacggtg tatcttccgg cggaagattc
     8461 cggacgcttg ttcgtcgcgc cgcatgtgca agaattggca cgtcagctca aggcgttttg
     8521 gcaggaggcg gaagccgtgt accggaagcg gcctgacggt cagggtgatg gaccaaccac
     8581 caaggccgca tagcttctcc cacgattgcc agccatgagt cacccctacg cggaagccac
     8641 gatacgatga tgaagttgta ccggtttctc ctgtttctgc ttcgggacgc caggaccatg
     8701 atgatcctca tggtcatgac ggggctgttg gccggtctct ccagcgtggg tttgttggcc
     8761 gtgatcaaca agctgatcaa cgggcccggc accgcctcgg agttgtttgc cgtggcattt
     8821 atcgggttgg ccgtcctcaa agtcgggtcg aattatctct cccagctctt gctggtcacc
     8881 ttcgcgcaaa agaccatctt aaaattgggc atggatctct gctggaaagt ggtgcgggcg
     8941 ccctatcgca cgctggaacg tcgcgggccg catgagattc ttgcaaccct gacggacgac
     9001 acgaacgctc tggcctgggc ggtgaacggg ttgccgggct tggcgatcaa tgtggcgatt
     9061 ctgggcggct gctcgctcta tctggcctgg ttatcctggc aggcgttcct aggcgtggtg
     9121 attctggccg tactgggatt gctcgggtat cgccagctct acaatcgtgt cctgcaatcg
     9181 tcgttggcgg tacgtgattc aaaaggcgtc ctcttcgaac atttccggag tttgacggaa
     9241 ggcatgaagg aactgatgct ccatcgcggc cgccgggaaa cgtttgtcga agaggatatc
     9301 cggcaagcag ccgacgcgct gcggcgtcat aatctcacca cgaccaagca gtatctcgtc
     9361 acggattcct ggacccaggt gctgttctac ggtttgatcg gcgtcatcct cttgctcttt
     9421 cctcgcgtcc tttcgctgtc cggtgaatcg ctgaccgggt atgccttcgc tatgctctac
     9481 atgatcggtc cgatgtgggg cctgctgggc atggtgccga cgttgagccg cgggcaggtg
     9541 gcgctggaaa agattgaatc gttggggttg gcgctggatg aaggcggtcg ggaaggtgga
     9601 gcggagcgtc cgatggcgca gggcaggccc tgtgtggaat tctccaacgc ggtgtttgcc
     9661 tacgaatcga agggcgagga tgaacggtca tttcgtttgg ggtcgctgga tctttccatt
     9721 cgcgcgggtg agctggtttt catcatcggc gggaacggca gcggcaaatc gacatttgtg
     9781 aaagtgctca cgggactcta tctgccgcaa caggggcagg tgacattgaa cggggaggcg
     9841 atcacgcccg cgactcagga ctggtaccgc caacattttg ctgcggtctt ttctgatttt
     9901 tacctcttta aaaagctgct cggactcgac ccgtcgttag tcgccagtca ggcggacgga
     9961 tggctgaaga ccctgcgtat cgatcacaag gtccgtattg aacagggcga atattcgacg
    10021 gtgaacttgt ctcaggggca acgaaaacgt ctcgccctgg tgactgccat gctggaagat
    10081 cggccgtttt acgtctttga tgaatgggct gccgatcaag atccgcaata taaggacgtc
    10141 ttctacggag aattgttgcc gaatctacgg gcccgaggga aaggggtcat tgtcgtnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagga acgaacgcgt gcggcccgct
    10321 ttgcgtttga gcgggatcgt caacgcttcg tgctctcgca tggagtattg cggcagctgc
    10381 tggcccgcta tacgcacata aaagcaggag agattcagtt catcactggc cggcatggaa
    10441 aaccggctct ggccgctcca tcagtcccgt ccgggaacat tcagtttagt ctttcccatt
    10501 cgggagaata tgcactcgta gctgtcgctc gcggccgtga agtcggcgtt gatgtcgagg
    10561 tgttcaaggc aaacatggac tgcgccaaac tggccgaacg cttctttgct ccgcgggaag
    10621 cgcaggcgat ggcaacgcta tgcggggaac cgcagcaacg catgttttat cgattgtgga
    10681 cggcgaagga agcctatttc aaagggctcg gtgtgggatt gtcactcggc ttggagcggt
    10741 gtgaaattgt gttcgatggg gaatcccctc acgcgcgtgt gcggctggcc gattccggaa
    10801 cctccgacca cgcgtggcag attcagtccc tgtcgctgcc ggatggcctt gcgggcgcgg
    10861 tggctgcgtc aggcggtgac tgggaattgc atcgctatga gtcgacggcc tactcgctcg
    10921 cctagccgga cttcctctgg gccgcatctc tcctcagtcc tcgaaagaaa tcctgcagca
    10981 gggcctggct gtcctccgca caaagcccgc cgaccacgtc tacgcgatga ttcagccttg
    11041 gctccggcgg aatatccatg atcgatccgc atgcgcccgc tttgggatcg aacgctccga
    11101 agaccagacg gggaatccgg gccagcacaa tggcgccgat gcacatgcaa cagggttcca
    11161 gggtgacata gagggtgctc tcggtgaggc gccagctgtt catctgtttg gctgcttcac
    11221 ggatgacaag cagctctgca tgtgccgtgg ggtcctgaac ggtttcccgc aggttgtgcg
    11281 ctcgggcgat gacgacgcca tcgcggacga ggagggcggc gatggggact tcgccgatca
    11341 gcggggcttc ccgcgcaagg ctgagggcct cttgcatgaa ttgtctatcg aggtcgtcgg
    11401 gcagggacat ggtgtcgtga gtgattcccg tcaacgagag cacgcatcgg gttctgacga
    11461 tgggcatgct agcatgtttg ggccgggaat cgcaggcgac gttcgttgga gggactgagg
    11521 ctagatttga ccgatcaggt agatggcccc cgacggttcg gggcgtccgc cgtccggttc
    11581 cacggtcacg gcgaatttct tcatccgggg aaactcaccc atattcttaa tcagcatgcg
    11641 ggctttctgg cctgcatcca ggccaaacac cccggcactg accggtttgt catcgatggc
    11701 ccagagttga tacaccttgc cgttcgggag cgctggtaag ttgaaggcgt agagccatgc
    11761 cttcttgctc gcgggatcga tcaggagaaa tgcgccggct gatttcgcca tgtcggagcc
    11821 ggatagtaag acgactttcg atgttggatt tttaaacagc ccgacaaatg tttcgctttg
    11881 ctgggcaagc cgctgtaatg aactgtcatg ctgagtgagt tgttgatgcg catcatccag
    11941 ctcggcctcc cgttgaagta attgatcccg caactcggcc acttcagtcg tgcgttgatc
    12001 caaatcagtc ttcagcgaat ccaaggtctg ttcgcgctgg cgcaattccg actgcagact
    12061 ggacgcgcgg gaggtttcgc gctgcaccgc ggcttgcaac tgctggattt cgcctgagcg
    12121 ctgggctgtc tgcgtgtagt tcatccaggc gagatatccg cctccaacaa ccaccacgaa
    12181 ggctgcaaat cccatggcga tgcggaacgg cagggaacgg gcaggcgtga tgggaggaaa
    12241 cagatgattc atccattcgc ccggttcgag gctggatcga ggtcccggct gctccgtttc
    12301 aggctggctt gtggtcgatg ccggggccat caggatcttg gctttgagcg tgttcggggg
    12361 cgttgcgggc gtcagtccga acggcagcag cgaagcgacg gtttgataat ccttcaaggc
    12421 ggcatgacag gcggcgcagc cggagagtag atgtgcctcg atggcctgcc gctcagagcg
    12481 ttcgagggcg ccgatcgcat agaggggaac cgcttcttcc agttcctcgt gggtcatagt
    12541 tggtgccctt gcggcagcgt ctcctgcaag gaggcgcgaa gtttcgacat ggccagtttg
    12601 attctcgtct tgacggtgcc caacggctga tttaatttcg ccgcgatctc cgtatgcgtc
    12661 agtccctgat aaaaagccat ttcgatcgct tgttgctgag gaagcggcag atcgagaata
    12721 gccttcgcca tcatttggcg catctcagtg tcttccctgg cttcatatgg attggggctc
    12781 acgtcgggcg tgacggagac cagcgggtgc tcgaacgaat cggcgacgac gtgctgcccg
    12841 cgcgaggcac gcgagcgcag ccgatcgatg gcacggctac gggtcagggt caccagccag
    12901 gcgatggggc ttcctcgacc gacatcgtat ttggcgattt ttcgccagac ttccaggtac
    12961 acatcctgga gcagttccgt cgcctcatcg cgatcgttga ggatccggta cgccagggta
    13021 aagagcaggg tggaggattg atcgtaaagc tggccgaatg ccgcttgatc ccccttcgcc
    13081 acgcgggcca ggagtttggg ttcaattgtg gatgcggctt gtcggggggc ggattccatg
    13141 cagtcggcct aagagcgcgg cagtcctcgg gatcagaatt gggcccacta taacactgtc
    13201 tgagaaaaac gactaatcag atgtaagttt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nacggcatgg tccggttcag caggatcgac gaacggttcc aggaccaggt
    13381 cccacgagag cgaacagagc ccgatcgtcg gaattccggc tgcagcgccg gccgcaatgc
    13441 caagatgtga cacatcagac agcaccaggc tgggaccggc ttggcggatg agcatagctt
    13501 ccgcttcgat cttcttttcc caatctgcat ggagggcgtg atgggctgcc caggtgcctg
    13561 ccacgtcgat gctcagcggc ccgttctgga tgcacccgat atcctgttgc gcctggctga
    13621 gttgccaagg aacggtcagc cgaggctcaa agaacccgga ggggactgat gtccggagga
    13681 tcgccttgag atcgggtacg agacgagcga gcgcgttgag caccggtacg acttgcgcgg
    13741 catggccgta gccgtggccg gaaatggagc accagatcaa cggcatcgca ggctgctgaa
    13801 aaaggccccc aacgtcgttc tcgactcgga atcctcctca acgtacctct gaacgtacgt
    13861 ctccgggtct tcctcgtctg cggcctcgtt ggatggcctt tttgagcacc ctagaagggc
    13921 tgtgcagttc agctccatct tgctttagct gttcgagtaa tcggattcca tcggagcgat
    13981 gttgtacatg agttccaaat cgaactcatc gatcagttcg ttcacggcgt cacggcccat
    14041 gtcagagcgg acctcgttga tgaccagttc gatggccatg gctgcgtgct gcccatattg
    14101 agtgatcgcg gattcaatcc gctgtctggc tgcgtcgaga gtcataagtc ctccagccgg
    14161 ccttagccga gtgttcgtaa caactgttgc agttccggca ccaagtccac gcccccgttg
    14221 gatcggccgg acacggccgc gtcaataccg gctttcaaga cgggcttggc atcctctttc
    14281 ttccccagtt tcacgagcgc gcgtccgagg gcaaggtgtg aagcgacgtg cgtcggatcc
    14341 aattgcgtcg ccaccgtcag atgctctgcc gcttcctcaa ggctgccgcc ttcctgcaaa
    14401 attttattgc ccagaccgta ccggcccaga aatcctttgg gatttttctc caccatttga
    14461 cgaaacgcgc tgatatccat ggcactcctt gtgaagcatc gttgttcaat cgagacgggc
    14521 gaattttagc atcaccccca tccgttcagc tatgggcggc gcgaagccgt gtgaggcgat
    14581 cccggtctgc cgtcgaaggg gtacgcccgg tgcccaaggc caggtgatgg agccgctcca
    14641 cgcaacggat agcagcaatg gtgtcctgcc gttgctcata gaggtcggcg agttcccgca
    14701 acaccgcggc ctcgccggac gggttgccga gcttgatgaa atgttcggtg gcattgttga
    14761 aacagcgttc cgcctccaca gagcgtccac tgtccagaaa catttttccc atctgactgt
    14821 aggttcccgc caggccttct tcattgccca ccacgcgatg ggaatccagg gcctgggtga
    14881 acagccgcac ggattcatcc cactgctgtt gccgggcttc ttccagcgcg agattgtggt
    14941 agagaatgcc cagcgctcga tggtcattga gtggtttcaa caggtccagc gcttcgagat
    15001 agtagggacg ggctttctcc ggtttatccg cgccgatgtg gagattgccc agatttacga
    15061 gagtgtgggc aatggcatgc gggtcgcgct cgttgcgttg gatggcgagc acttctttgt
    15121 aacaggcatc agcttcctcg aaggcgccgg cgagtgccag ggtgttgcct agattgccga
    15181 gactattgga gagttcccgt ggcgttcccg taaggcgatc atccttcacc gcggcctccc
    15241 aggcggcccg ggcttggacg agatcccctc gatgaagaaa aatgcgcgcg cgatgtttat
    15301 cgttttcagc catacgtcag aaccgatgtt gcctctcgtt gctcgtctct cgcgtccgga
    15361 acggtttccc gttcacgggt gacgtctcgc ggcccacgtt agtctccgcc tttgggtgtg
    15421 tcttttcgaa actccactac gatgtcgtct tcctcatcca gtcccaagcc tgcgcgaaac
    15481 gagtcgagca attcggcgac atgcgcgagg tgccgtggat ccttcccatc aggcgaggcc
    15541 aggtaggatt cggccagagt catgccggtc tgaaagtgac gggcgatcgt ctcttcggtg
    15601 acctgctgcc aggtgatgta aatgcagaag gcctgaaacc gatgggcctt gggatgtcgg
    15661 gcggccagcg cgagaagctg ttcgtaggcg tctctggcgg tcgagccggc attggaggaa
    15721 aacgtctcct ccaattcagt ggcctggtcc cagtcgacac caagctcggt ttgcgcttcc
    15781 tgtaacgttt cttgtgcggc caacgcggcg tcgaattgca tgcagtcctc aacgggattg
    15841 atgtggggct tccggattga ttgcagaata ctgggtgcgc ggaagggagg tcaatgttgc
    15901 tgatccagaa gtggcccggt ggaacgggtt catcaggtcg atcgggaggg aaacaggcct
    15961 catgtatagg ccgaatagcc ctcgcgcttc agaaaagacg ggccgcgacc gataaagata
    16021 gccgggggcc cctgtgcagg gcgcacgcaa cgcgcaccaa ggaggatcgt ctatcatggc
    16081 atcggcgcaa gaacttgttc agtcctgctc aaatgtcttt accctgccgg agatatattt
    16141 ccgtgtccgc ga
//
LOCUS       DAOQDS010000080        15844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1555_length_15844_cov_21.110837, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000080 DAOQDS010000000
VERSION     DAOQDS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15844
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1555_length_15844_cov_21.110837"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PK589_15785"
     CDS             <1..894
                     /locus_tag="PK589_15785"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNJ21121.1"
                     /translation="RNMVKVVRTEYPQRSGLIAINLAAIIFGTAALYGRLDIAPLWIV
                     TMRGAFASVALAGVGLLSGGLGLRPTIAQLKRLLVTGILLAIHWVTFFQSVQLAGVAI
                     ATLTFAVFPLFTVLLETMKARRRPSLLELSAGVAIVAAVALLVDTKDSDATLTGTIVG
                     LGSALAFAVFGIKAQILPTEITPLMVSLIQNLVVSLSLTPLLAVSSPAPKTTTDWLGL
                     LLLGVVTTAFMHQLYLFALKRLSATTCSGFIALEPVYAIVFAAVFFGEPLTLWVVVSA
                     VLIIGASVVLLRAERVTVVLD"
     gene            1228..1359
                     /locus_tag="PK589_15790"
     CDS             1228..1359
                     /locus_tag="PK589_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21122.1"
                     /translation="MIEFPLILIGYALLPIIAIQLMIMADRPQQKGEQNAFPQVEIL"
     gene            1688..2371
                     /locus_tag="PK589_15795"
     CDS             1688..2371
                     /locus_tag="PK589_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21123.1"
                     /translation="MKSANALIGLVLLLLATTGCHGYTLLETPTTDGFHSKLPAPYTR
                     AVVWGGRPDTVQSASTWLLKKGLLVVDQTKVLQAAADQKVSLSGYQYLETDVLRMAKL
                     VGARLVVFSNAEVGSWEALDWSNGVPRSQRVYSATIALRAVDVNTGEIEWSGKAQSTE
                     RFSNIEEGISFLTCHALATAWGMRNPGSLAPENICPPGSGLMTTEGTKHHATPAASTR
                     ENLTPSMNN"
     gene            2521..3372
                     /locus_tag="PK589_15800"
     CDS             2521..3372
                     /locus_tag="PK589_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21124.1"
                     /translation="MVRYPLIFLLSWSFFAAPIAEANENHPFWTEQALFHFGEDVFFT
                     GRASCAPNVEEGRQRAYLAAVQEVKNFTRAQEIGGFPLDTQMIFEDSRPADCAPGLVT
                     VWRLLRAPRLALDGLARRGTPGKSADISPILAQVAAVRNLTPRVGMLRDEVWNRYGLP
                     RSIWARPEKGELIWDYSQFGLTIVFNQEDVLTRWRLTGPNPRSSEDTSPAPSQPEPLS
                     GKDLPAIDLTARLQQLEEQQDLERRHEAQRYCSLRFAEVPDTLRPTQGTCEQREYQRL
                     KERHPRF"
     gene            3410..3904
                     /locus_tag="PK589_15805"
     CDS             3410..3904
                     /locus_tag="PK589_15805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ21125.1"
                     /translation="MTTDKIAHATILLVDDDPSTLLLASKPLQDQGYTVLQAPGSAEA
                     LRLYAEHPRPIHLVVTDIFLPPPGFQLSVEENPYPRVNGLEMVERLLEKKRDIRIILM
                     SSTPGPDLQGRGLIREGLPFLKKPFTSDALLTLIRDTLAGPPATQGPTKPSASGKGEV
                     EWVD"
     assembly_gap    4037..4136
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4493..5110
                     /locus_tag="PK589_15810"
     CDS             4493..5110
                     /locus_tag="PK589_15810"
                     /inference="COORDINATES: protein motif:HMM:NF037406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21126.1"
                     /translation="MAPTTTSASPAPQPEPPAPPIVAQVSAPVRSSAPASSQIPIPEK
                     VPEAKTSAEHGTLEILRRVCARFHLVARQLRLRKEYRPTLEITDEYDLQDLFYALLRL
                     QFDEVGTEEWTPAYTNGAPRTSYLLDWEKTVVVVKQTRSGLTTKDLAEQIAADTARYS
                     ARPNGANFVCFVYDPDGRVGNPRGLEADLSSTEGTYRVEVIVAPK"
     gene            5231..5485
                     /locus_tag="PK589_15815"
     CDS             5231..5485
                     /locus_tag="PK589_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNJ21127.1"
                     /translation="MPDRAYVLINVHPGQTADVVKALADIKEIKQIDPCWGKPDIFTI
                     VEIPHQDALTQLVLAKIHAIPGVEQTDTHNVYRLQETKPK"
     gene            5482..5919
                     /locus_tag="PK589_15820"
     CDS             5482..5919
                     /locus_tag="PK589_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21128.1"
                     /translation="MIRMIAPLLFAIVVAGCAGAPAAPELDVMEVTLEAPADKVQTAV
                     ADVLTQGGYSVDQDESGNVTTGMRQEVRGPWNNLLRWRFGVGKSRVEARIVRQDVSTT
                     RLRLQVFYRGKDGIFDTWQDAETPLPQSAANEIRLIKNALRLL"
     gene            complement(5926..7320)
                     /locus_tag="PK589_15825"
     CDS             complement(5926..7320)
                     /locus_tag="PK589_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ21129.1"
                     /translation="MNQAKLFVVDDDETARTLLAEALAKEGYAVEAFASGQAAVERGK
                     QAPPDVVLTDIRMEQGDGFLVLKEFKRFSPDTSIVLLTAFGSLEGAIEAIKQGAYDYL
                     AKPFKRDEIRLVVQRSLEHCRLVRENARFREDARVREPWSHLVGSSPAMLEVYKLVAR
                     VSDGRSTVLIEGESGTGKELIARAVHLNSPRREKPFIPVNCGALPDHLLESEMFGYEK
                     GAFTGAVGAKAGLFEAANGGTLFLDEIGDLGQALQVKLLRVMQEQEVRRVGSTSSVKV
                     DVRIIAATNRDLAARVKEGKFRDDLYYRLNVVRIALPSLAQRQEDIPMLAHHFLQKYA
                     KQSSHVRGFAPDTMALLKRYQWPGNVRELENAVERAVSLSHGPLLLPEDLPESIRAEP
                     GPSDQPAGGLSEGDPDALLTLDEVEKRHLSRVLKETRGNKVKAAKILGIDRRTLYRMA
                     ERFGLDFGDESDEK"
     gene            complement(7334..>8440)
                     /locus_tag="PK589_15830"
     CDS             complement(7334..>8440)
                     /locus_tag="PK589_15830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ21130.1"
                     /translation="FLIVTVMEWMFPLNVVGAFGFVLPILLVATVRNRQLMVMTLALC
                     ILVTFIGLFQPAKKKERFTTVVFNRIMVVFVLAGVAYIGVTWEERKAREEAARAALAT
                     QTEHLLRANTQLGELKDTLARSERLAAVGQLVASVAHEVGTPLHSIAWHVEALAEEPG
                     LTAEMQKRIGIIDEQLNRVVRIIQDLLSSTRQRKPEPTWYPAERLIEPVVALMEPGYH
                     AKGVALQAEVTPAVSVWADAEKIHQVLVNLLTNALAATSTGGQVVVSVTARPASAEER
                     ERGSCTGRTDLDTTVLLVVSDSGCGMPREDMDRAFQPFFTTKAIGKGTGLGLFLSREV
                     VVAHGGQLTLESEVGKGTTATVILPGLPTHPGAA"
     assembly_gap    8441..8540
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8541..10801)
                     /gene="malQ"
                     /locus_tag="PK589_15835"
     CDS             complement(<8541..10801)
                     /gene="malQ"
                     /locus_tag="PK589_15835"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNJ21131.1"
                     /translation="MKVFTHTRSTTLMYDGTEQDLLCLLASRAGISPEYYDIAGTLHV
                     TSDDTRRAILSAMGFDTTSRESLATTLADWDEASWRQPCDPILILEQGYGPTRWAFRL
                     PLEEKEEAQLVLTWHLCDEKGTVVHREDAGPRLIPQEVHVAAGRRMVRLDLPLVPNLA
                     LGYYDLSVVGTVGTLVTKAVLRVVVAPPHCYVPPGMAQGQRVWGLAVQLYALRSRTNW
                     GVGDFTDLARLVEWAGKELGAGIIGLNPLHALKNSRPHHLSPYSPTSRLFLNELYIDL
                     DRLPEYHASPDAQRLRQSPEFQSGVEQARAADFVDSESVVKAKRTMLDLAYRQFLTEN
                     YAGTEPSLQPTSARGWLLERFIRDEGESLERFALFQVLEEDRRLIEQAPKLWPEWPEQ
                     YRNPGTPALREFARRHRKRVRFFQYVQWVAADQLRAANTRASQVHMTVGLYPDLALGS
                     DRNGAEAWMLQDTLALGADCGAPPDAFAPQGQNWGFAPFNPLRLKATGYRSFIELLRK
                     TLRQGGAIRIDHVMMLFRLFWVPRGLTAAAGAYVQYPAEDLLRILALESMRAQTLVIG
                     EDLGTVPDYVREQLARYKILSYRVFYFERNWDGSCKPPSAYPDQSLAVVTTHDLPTLT
                     GYWSGEDIRLRARLGMYVHEQAVQQAFEERARDKNQFLAALQSAGLLPPGVSAQQESV
                     PAMTPDLCRAIHLYLAGSPAWVVLANLDDVIGEVTQMNLPGTVDAYPNWSRKLSLSLE
                     ELQQDERVQALAAA"
     gene            10860..11882
                     /locus_tag="PK589_15840"
     CDS             10860..11882
                     /locus_tag="PK589_15840"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNJ21132.1"
                     /translation="MSVDQASFQDRRPAARDGTSPFQAMYSMSNRLLVRFGTSSWAYE
                     GWRNQIYHRPYASSRFSKDSLAEYATYPTGGTPLFRTVGIDHSFYRPASATQLAHYAS
                     QVPADFHFCSKVWEELTVPAYADLPRYGAKAGKPNPRFLDSHLFREMVLQPFLEALPG
                     HIGPFIFEFQRTGMDPVVFLDALDRFLADLPAGFPYAVEIRNPAVLSPRYRDILLAHQ
                     VAHTYNHWTGMPPLLTQHRLLNERFTAPFVVMRLLTPLGLPYADAVERYAPYNRIVTP
                     QPQMRREAASLIHSASSQGVTPYVLVNNRSEGNAPLTIQAIVEMVTALPDSPALLPSP
                     SRDSMI"
     gene            11944..12675
                     /locus_tag="PK589_15845"
     CDS             11944..12675
                     /locus_tag="PK589_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21133.1"
                     /translation="MTPLLSMFRSGVLFAVAVHCLAVAIPVQAASDHSRVDITGDYRY
                     AYHQPETASEAKQHACREALHQAISTSTAVREHTASIVDSKLFHTLVHTLAAQHVTDQ
                     QILQQSEQGRTVYCKIKAVFQADAVERVLLAQTTSGSEQALDQNRALRILAAHEEPDG
                     MLVITYQAIKRLDWLGTAYQGGLRESADVMVDFYDEQGILVRSSRHPARKTATGDDVM
                     NPGEVGTLKVAKPLNGKTFRVWVVK"
     gene            complement(12758..13585)
                     /locus_tag="PK589_15850"
     CDS             complement(12758..13585)
                     /locus_tag="PK589_15850"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNJ21134.1"
                     /translation="MRQGAWSVLGIMGVMLVTSGCVAIEAGHEGVMVEQPFFFGHGGV
                     DPAPTKTGRVWVAPTTKVIEVDIRPLQYSEHFDIISAENAPVSFDAFLIANVVEGRTP
                     ELIGRYGTTWYQNNVKEAFRTFVREEVQRYPLFQLTTDPTTRTKLQDAIAREVQNKLI
                     DKQNMPIRLNRVVVGSILPPKGVVEQTTQTIVQEQRKITMVEFQKAEEAREKAEKQRG
                     IADRAYRESLGLTAPEFVDLRRIEVQKEIVQHAPSALTVIMGLERVGINMPSSGADR"
     gene            complement(13770..14192)
                     /locus_tag="PK589_15855"
     CDS             complement(13770..14192)
                     /locus_tag="PK589_15855"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ21135.1"
                     /translation="MNLNRSTHIRMKAWMAGIGLVVLVAALPACESNATNQDAGKPAG
                     GATPADVQVGEAKFSANCAVCHGVRGVGTKQGPPLVHKIYEPNHHADMAFQRAAENGV
                     RAHHWEFGNMPKIEGVTPADVEQIIRYVRWLQREAGIY"
     gene            complement(14189..14365)
                     /locus_tag="PK589_15860"
     CDS             complement(14189..14365)
                     /locus_tag="PK589_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21136.1"
                     /translation="MTPEEFFMYLIIALVIIAPIGARLYRRLTLNRTTQMLREQLHSS
                     QSKPSSTTPPELPR"
     gene            complement(14410..15108)
                     /gene="queC"
                     /locus_tag="PK589_15865"
     CDS             complement(14410..15108)
                     /gene="queC"
                     /locus_tag="PK589_15865"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNJ21137.1"
                     /translation="MTPLQPKAVVLLSGGLDSTVTAAIAQQDGYSIHCLTVSYGQRHQ
                     VEVERARAVAAALGTAGHVVVTVDLRAIGGSALTADVDVPKDRPPEARASEIPVTYVP
                     ARNTIFLSLALAYAETVKASAIYFGANVLDYSGYPDCRPDFIRAFETVARLGTKMGVE
                     GHGAEVRAPLLMMSKADIVRRGQALNVPFELTHSCYDPGVEGLACGRCDSCRIRREGF
                     RLAGVVDPVPYAIL"
     gene            complement(15229..15804)
                     /locus_tag="PK589_15870"
     CDS             complement(15229..15804)
                     /locus_tag="PK589_15870"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNJ21138.1"
                     /translation="MATKVGAKKKTPTTKAKAIAPEKPVKKERPAPVTTMTEKDEDSV
                     AARVVAALTLKETPKEKEERQRRREVLQRMLLGKRQEIMREIEGNLGQSLTEDQQRRL
                     ESARDVGDQALMDLDRELGISLMEMRNRKRQAIDEALTRLSEGTYGICAECGIEVSEK
                     RLEAVPFAKLCVQCQSRQELLEKIEKEEDRD"
BASE COUNT         3266 a         5001 c         4184 g         3193 t
ORIGIN      
        1 agaaatatgg tcaaggtcgt caggacagaa taccctcaga gatcaggact gatcgcaatc
       61 aatcttgccg ccatcatctt tggcacggcc gcactgtacg gcagattaga tatcgcacca
      121 ttgtggattg tgacgatgag aggagcgttt gcttcagtgg cactagccgg agtgggctta
      181 ctaagtggcg gcctgggtct gcgtccaacc atcgcacaac tcaagcgcct gctcgtgaca
      241 ggcatactgc tggcgattca ttgggtcacc ttcttccaat cagtccagct tgccggtgtg
      301 gccatcgcga cactgacgtt cgcagtcttt cctctgttta cggtgctgct tgaaacgatg
      361 aaggcgcgcc ggcgcccaag cctgctagaa cttagcgccg gggttgcgat tgttgcggca
      421 gttgcgttgt tagtggatac gaaggattcc gatgccacac tcaccggcac gatcgttggg
      481 ctcggttctg cattggcgtt tgcggtgttt gggatcaagg cacagatcct ccccaccgag
      541 ataacacccc tcatggtgtc actcattcaa aatctcgtgg tgtccttaag tctcacgccg
      601 cttctcgcgg tcagctcgcc agcgccgaaa accaccactg actggcttgg cctcctgctg
      661 ctcggcgttg tgacgacagc cttcatgcat cagctctacc tgtttgcgtt gaagagattg
      721 tccgccacga cgtgcagcgg gtttattgcc cttgagccgg tgtacgcgat cgtctttgct
      781 gcagtatttt ttggggaacc cttgacgctc tgggtcgtgg tgagcgcggt gcttattatt
      841 ggtgcatctg tcgtgttgtt acgagccgag cgagtcacgg tcgttctgga ctgactattc
      901 atcacaggtc cgaagcgcca ccagcccctc agaacagcag cccaagatct gccatcttct
      961 ccccccttct cctacgcctc tgcaacgcga gcccccatct cttggatcgc aaggactcag
     1021 gccgtctttc gtcccgttac ccttaggtct tccggctcag cgcaaattgt tctcattgca
     1081 cagttctctc ccttgagttt cggcctccaa aggagcactg ccggcataaa ccatctcgag
     1141 catcagtaca atcaatagct tggcactccg tgggagaacg gtatcgagct tgcagtggag
     1201 gctctacata acaaaaggag ttccaccatg attgagtttc cgctgatcct tataggctat
     1261 gcacttctgc cgatcattgc cattcagttg atgatcatgg ctgaccgtcc ccaacagaag
     1321 ggcgaacaaa acgcctttcc acaggtcgaa atcctgtaag cctgcggcgg cttcacaagc
     1381 catgagtgaa gccgccgcct ttccaggccg tcgttgactc ccgcctcctt cccgactgcg
     1441 gcaattcaca tcccagactt gcgttgagca ccaccgccgg ccgaggctcc cacaatttca
     1501 tcctgtaagt aagtagcggt atctcgagag cgccaacacc caatgtactc gacgaatgca
     1561 gcagacgtag gtagaatcaa tcctgtataa tgaggccgaa acattgagtg cccatccagg
     1621 aagcacgaac cgttcttgat aaagattcat ggcgagcagt cagcacacat ccttccataa
     1681 agagtccatg aaatcggcga atgcattgat cggtttggtt ctgctcctcc tcgccacgac
     1741 cggatgccac gggtataccc tcttagaaac tcccacgact gatggatttc actctaaact
     1801 tcctgcgccc tacaccagag cggtcgtttg gggcggacgc ccagatacgg tgcaaagcgc
     1861 aagtacctgg ctgcttaaaa aaggtcttct cgtcgtagac caaaccaagg tcttgcaagc
     1921 cgcagccgat caaaaagtca gtctttcggg atatcagtat ctcgaaacag acgtgcttcg
     1981 aatggcgaaa ctggttggtg cccgactggt ggtctttagc aatgccgagg tagggtcttg
     2041 ggaagcactg gactggagta acggggtacc gcgcagtcaa cgcgtgtact ctgcgacgat
     2101 tgcgctgcgg gccgttgatg tgaacaccgg cgaaattgaa tggagcggga aagctcagtc
     2161 caccgaacgc ttcagcaaca tcgaggaggg aatcagcttc ctcacctgcc atgccttagc
     2221 cacagcctgg ggcatgcgta accccggctc cctcgctccc gaaaacatct gtcctccggg
     2281 gtccggcctc atgacgaccg agggcaccaa gcaccacgcc acccctgcag cctcgacgag
     2341 agaaaatctt accccctcca tgaataacta ggccgcgcat ctcttcgcgg gctattgcct
     2401 cgccgacgct ctttcctaca gtgcctctcg cacaaactcg ctcttttgtc gattttcttg
     2461 tccggcgcgt tatactgcat gagtgcctcc ctcttacggc tcttttattg agtgttgcct
     2521 atggtgcggt acccgctcat ttttctcctg agttggagct tttttgcggc gccaatcgcc
     2581 gaggccaacg agaatcaccc gttctggacg gaacaagccc tctttcattt cggtgaagat
     2641 gtctttttta cggggcgagc ctcctgcgca cccaacgtag aagaagggcg gcaacgcgcc
     2701 tacctcgccg ctgtccagga ggtcaaaaat ttcacgcggg cccaggaaat cggcggattt
     2761 ccgctggaca cccagatgat ttttgaagac agccgtccgg ctgactgcgc cccgggattg
     2821 gtgacggtct ggcgcctttt acgtgcgccc cgcctggccc tggacggact cgcaagacgc
     2881 ggcacccccg ggaagtcggc cgacatttcg ccgatcctcg cacaagtcgc agcagtgaga
     2941 aatctcacgc ctcgtgtagg aatgttgcgt gacgaggtct ggaatcgata cggcctgccg
     3001 cgatcaatct gggcgcgccc cgaaaagggt gaactgatct gggattactc gcagttcggc
     3061 ctgaccatcg tcttcaacca ggaagacgtc ctgacgcgct ggcggctgac cggacccaat
     3121 ccacgatcca gcgaagacac ctccccggct ccatcccaac cggagcccct cagcggcaag
     3181 gatcttcctg ctattgacct cacagcccgc ctccagcaac ttgaagaaca gcaggacctc
     3241 gaacgccgcc atgaagccca gcgctattgc tcgttgcgtt tcgccgaggt gccagacacg
     3301 ttgcgtccca cccaaggcac ctgtgagcaa cgggaatacc aacgactcaa ggagcgccat
     3361 ccccgatttt gatccgtctc ccgagtcact gccacgaagg agggccggca tgaccaccga
     3421 caaaatagcg cacgcgacaa tcctcctcgt tgacgacgac ccctcaacgc tcctgctggc
     3481 gtccaaacca ctgcaggatc agggatacac ggtactgcag gcaccgggca gtgccgaagc
     3541 gctcaggctc tatgcggaac acccaaggcc catccatctc gtcgtcacgg acatctttct
     3601 cccacccccc ggcttccaac tttccgtcga ggagaatcct tacccccgcg tgaatggtct
     3661 cgagatggtc gaacgcctgc tcgagaaaaa gcgagacatc cgcatcattc tgatgtccag
     3721 cactccagga cctgacctgc agggccgcgg ccttatccgt gagggcttgc ccttcctcaa
     3781 aaaaccgttt accagcgacg cgctgctgac actcatccgt gacaccttgg ccggacctcc
     3841 ggcgacacag ggcccgacaa aaccttctgc atccggcaag ggagaggtcg agtgggtcga
     3901 ctgacccgga tccatcctca ccggcacccc cacgtcgacc acgagtaaaa gcagggattg
     3961 cttgtcaggc tccaccccct cggatagact tttctcacga gagggagact cgcatggcac
     4021 gagcaaaagc aaccgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncaat
     4141 tgtcccggca ccatcgcctg gaaccagcgg gagcagtgcg aaggccgcgg gagatgcaac
     4201 gattcctaca accaccattc ctccaatcac atcggcaaga agtaccgtgc ctcaaggcac
     4261 ccttccaccg gttccccaca cgaccttgcg accgcgacca gcaccactcg tcagtaccga
     4321 cattcccgct tcaactcagc tgtcaacgaa tgttccgccc cccctgacgc cgacgcctgc
     4381 tccggcttct gagccacccc ggcagatagt tccctcggta ggtgcaacca cggcagactc
     4441 aagccctatt cctgttcaag agaaccgacc cacgcctccg ctcgttcaga ccatggcgcc
     4501 gacaacgact agcgcaagcc ctgcaccgca gccggaaccg cccgctccac ccatcgtggc
     4561 gcaggtatcc gcaccggtcc gatcatcagc gcccgcatcc tcccagatcc ccatcccgga
     4621 gaaggttccc gaggcgaaaa cctccgccga gcatggcacg ctggaaatac tgagaagagt
     4681 ctgcgcccgg tttcacctcg tcgcccgaca actgcgactc agaaaagaat accgtccgac
     4741 gcttgaaatc accgacgagt atgacttgca ggatctcttc tacgcgctcc tccgattgca
     4801 gttcgatgag gtcggcacag aagagtggac tccggcctat acgaacggcg cgccgcggac
     4861 cagctatctc cttgattggg aaaagacggt cgtggtcgtc aaacagaccc gctccggact
     4921 gacgacgaaa gatctcgccg aacagatcgc cgcagacaca gcccgctact ccgcgcgacc
     4981 gaatggagcg aacttcgtct gctttgtgta tgacccggac ggacgtgtgg gaaatccgcg
     5041 tggattggag gctgacctct catccactga ggggacatac cgcgtcgaag tcattgtggc
     5101 gcccaagtga catcgcatcg tcaccaaggc agacgtgcgt gcatcgtgtt gacttcatcc
     5161 ggagcaatcc gataggtttc gattcgcgtg aggcctaacc acctgcgcta gttcaaggga
     5221 gatcctctcc atgcccgacc gcgcctacgt attgatcaac gtccatcccg gtcaaaccgc
     5281 tgatgtcgtg aaggccttag cggacattaa agaaatcaaa caaatcgatc cctgttgggg
     5341 caagcccgac atcttcacca tcgtggaaat tcctcatcag gatgccttaa cccagcttgt
     5401 gctggcgaag attcacgcca ttcctggcgt cgagcaaacc gatacccaca acgtctatcg
     5461 gcttcaggag acaaaaccca aatgatccgc atgatcgcgc cgcttctgtt cgccatcgtg
     5521 gtagccggat gtgccggagc ccccgccgct cctgagctgg atgtgatgga agtgacgctg
     5581 gaagctcctg cagacaaggt ccaaaccgct gtcgctgatg tgctcacaca gggaggctac
     5641 tccgtcgatc aggatgagag cggcaacgtg acgactggaa tgagacagga agtcagaggt
     5701 ccgtggaaca acttgttgcg ctggagattt ggtgtaggaa aaagccgggt ggaagcgcgc
     5761 atcgtgcggc aagatgtcag caccacacgg ctacggctgc aggtgttcta tcggggcaaa
     5821 gacgggatct tcgatacttg gcaagatgcg gagaccccct tgccgcagag cgccgccaac
     5881 gagatccgct tgatcaagaa cgccctgcgg ttgttgtagt ccagactact tctcgtcact
     5941 ctcatcgccg aaatccaacc cgaatcgttc cgccattctg tacaacgtcc gccggtcgat
     6001 tcccaggatc ttcgccgcct tgaccttatt gcccctggtc tctttcagca cccgtgagag
     6061 atgacgcttc tctacttcat ccaaggtcag caacgcgtca ggatccccct cggacagtcc
     6121 accggcaggc tggtcggacg gaccgggctc ggctcggatc gattcgggga gatcttcggg
     6181 taacagcagc ggaccatgac tcaaggacac ggcccgttcc acggcatttt ccagctctcg
     6241 cacattgccc ggccactgat agcgcttcaa caacgccatc gtgtcagggg cgaaccctct
     6301 cacatgggaa gattgcttgg catacttctg taaaaaatga tgggcgagca tcggaatatc
     6361 ttcctgtcgc tgagctaacg agggcagtgc aatccgcacc acattcaacc gatagtacaa
     6421 atcatcgcgg aatttgccct ctttgacccg ggcggcaagg tcccgattcg tggcggcgat
     6481 tatacgaaca tcaactttca cagaggacgt gctgcccacc cgccgcacct cctgttcctg
     6541 catcacacgc aacagcttca cctgaagcgc ttgcccgaga tccccgatct cgtccaaaaa
     6601 cagcgtcccg ccattggccg cctcaaataa tccagccttc gcgcccacgg ctccggtaaa
     6661 cgcgcctttt tcataaccga acatttccga ttcgagcaga tgatcgggaa gagcaccgca
     6721 gttcaccgga ataaatggtt tctcgcgacg cggactgttt aaatgaaccg cgcgggcaat
     6781 caactccttg ccggttccgc tttccccttc gatcagcacc gtgctccgcc catcggatac
     6841 gcgggcgacg agcttgtaga cctccaacat cgccggactg ctgccaacca gatgcgacca
     6901 gggttcacgc acgcgggcat cttcacgaaa ccgggcattt tcccgaacga gacggcaatg
     6961 ctccaagctg cgctgcacga ccaggcgaat ctcgtcccgc ttgaagggtt tcgcgaggta
     7021 gtcataggca ccttgcttga tggcttcaat cgcgccctcc aaggatccga acgccgtcag
     7081 caacacgatc gaggtatccg ggctgaatcg cttgaattct ttcaacacca aaaacccgtc
     7141 gccttgctcc atgcggatgt cggtgagcac cacgtcggga ggcgcctgct tgccacgctc
     7201 caccgccgcc tgcccgctcg caaaggcctc gaccgcatac ccttctttcg cgagcgcttc
     7261 cgccagtaag gtccgggcag tctcatcatc atcgaccaca aacagttttg cctgattcat
     7321 cagccttgcg ctcctacgct gcgccgggat gcgtcggcag cccgggaagg atcacggtcg
     7381 ccgtcgtccc ttttccgact tcactctcca gtgtcagctg accgccgtgt gccacaacga
     7441 cttcccggct gagaaacaat cctaaaccgg tccctttccc gatcgccttc gtggtaaaaa
     7501 acggctgaaa ggcccgatcc atatcttcac gcggcattcc gcatccggag tcggacacca
     7561 cgagcaggac cgtcgtgtcg agatcagtcc ggcccgtgca ggatccgcgt tcccgctcct
     7621 ccgctgatgc cgggcgtgct gtcaccgaca ccaccacctg ccctcctgtg ctggtcgcag
     7681 ccagcgcgtt ggtgaggaga ttcaccaaga cttgatgaat cttttcggca tccgcccaca
     7741 ccgacaccgc cggcgtgacc tccgcctgaa gcgccacgcc cttcgcgtga taccccggct
     7801 ccattaatgc caccaccggc tcaatgagcc gctcggccgg ataccaggtc ggttcgggct
     7861 tacgttgtct cgtcgacgag agaagatctt gaatgatccg taccacacga ttcaactgtt
     7921 catcgatgat tccgatccgc ttttgcatct ccgccgtgag cccaggctct tccgccaacg
     7981 cctccacatg ccaggcgatg gagtgcaagg gcgtccccac ctcgtgcgcc accgatgcga
     8041 ccaattgccc caccgccgcc aaccgctcag accgcgccag tgtatcctta agttcgccca
     8101 actgcgtgtt ggcgcggagc agatgctccg tttgagtcgc caatgcagcc cgggccgctt
     8161 cttcccgggc cttccgttcc tcccaggtca cgccgatata ggccacccca gccagaacga
     8221 agacgaccat aatgcgattg aataccaccg tcgtgaaccg ttcttttttc ttcgccggct
     8281 gaaacagccc gatgaatgtc accaggatac agagcgcgag cgtcatcacc atgagttgcc
     8341 gattccgaac ggtggccacg agcaatatcg gcaacacaaa cccgaatgcg ccgaccacat
     8401 tcagcggaaa catccattcc atcaccgtca cgatgaggaa nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn gcggcggcga gagcttgtac acgctcatcc tgttggagtt
     8581 cctccagcga cagcgacaat ttgcgtgacc agttcggata ggcgtctacg gtaccaggca
     8641 aattcatctg tgtcacttca ccgatcacat catcaaggtt ggccaatacc acccaggctg
     8701 gcgagccggc aagatacagg tgaatcgccc ggcacaagtc cggtgtcatg gccggcacag
     8761 attcctgctg cgcgcttaca ccgggaggca acagcccggc gctctgcagc gctgcgagaa
     8821 actgattctt gtccctggcc cgttcctcga aggcctgctg taccgcctgt tcatgcacat
     8881 acatcccgag cctggcgcgc agtcggatat cttccccgct ccaatacccg gtcaatgtcg
     8941 ggagatcatg cgtcgtcacg acggcgagcg attgatcggg ataagccgac ggcggcttgc
     9001 aggacccgtc ccagttccgc tcgaagtaga acacgcggta ggacaggatt ttgtaccggg
     9061 cgagttgttc gcgaacataa tccggcaccg tacctaggtc ttcaccaata accagggtct
     9121 gcgcacgcat gctctccagc gcaagaatcc gcagcagatc ttcggccgga tactgcacat
     9181 aggctccggc tgcggcagtc aacccgcgag gcacccagaa cagccggaac aacatcatga
     9241 catgatcgat gcgaatcgct ccgccctggc gcaaggtctt ccggaggagc tcgatgaacg
     9301 aacgataccc tgtcgccttc agacgcagcg ggttgaacgg cgcgaatccc caattctgcc
     9361 cctgtggagc gaacgcatcc ggtggagcgc cgcagtctgc acccagcgcc agcgtatcct
     9421 gcaacatcca ggcttctgcg ccgttgcgat cgcttcccaa cgccagatca ggatacaggc
     9481 cgaccgtcat gtgcacctgt gaggcgcggg tattggcggc gcgaagttga tcagccgcga
     9541 cccactgcac atactgaaaa aacctaacgc gtttgcgatg gcgccgcgca aattcccgca
     9601 acgccggcgt gccgggattc cggtactgtt ccggccattc aggccagagc ttcggagcct
     9661 gttcgatcag acggcggtct tcttccagca cctggaacaa ggcaaatcgt tccagcgact
     9721 cgccttcatc ccggatgaat cgttccaaca accatcccct ggcactggtg ggctgcagcg
     9781 acggctctgt tcccgcataa ttctccgtca ggaattgccg ataggccaaa tccagcatcg
     9841 tgcgcttggc cttgaccacg gattcagagt ccacgaagtc tgccgcgcgt gcctgctcca
     9901 cgccgctttg aaattcaggg ctctgtctca gcctctgggc gtcgggcgac gcatggtatt
     9961 caggcaggcg atccagatcg atatagagtt cgttcaaaaa gaggcggctt gtcggagagt
    10021 agggactgag gtggtgaggg cgcgaattct tgagcgcgtg taagggattc aatccgatga
    10081 tccccgcccc caattccttg cccgcccatt cgaccagacg agccaaatcc gtaaaatcgc
    10141 cgacgcccca attcgtgcgg gaacgcaatg catagagttg caccgccaat ccccacactc
    10201 gctgcccctg cgccatccct ggcggcacgt aacaatgggg gggagccacc acgacacgga
    10261 gcaccgcttt cgtgaccaag gttccgactg tgccgaccac cgaaagatcg taatagccca
    10321 gcgctaagtt cggtaccagc ggcaagtcga ggcggaccat tcgccgaccg gccgccacgt
    10381 gcacctcttg gggaatgagt cgcggaccgg catcctcgcg atgaacgact gtcccttttt
    10441 cgtcgcagag gtgccatgtc aggactaact gcgcctcttc tttctcctcc aggggaagac
    10501 gaaaggccca tcgagttgga ccatacccct gttcgagaat cagaatcgga tcgcagggtt
    10561 gtctccacga ggcctcgtcc cagtcagcca aggttgtggc caacgattct cgcgacgtcg
    10621 tgtcgaaccc catggcagaa aggatggcgc gacgcgtgtc gtcgctggtc acatggagtg
    10681 tcccggcgat gtcgtaatac tcgggggaaa taccggcccg cgaggcgagg aggcacagga
    10741 ggtcttgctc ggtaccgtcg tacataagtg tcgtggaccg agtgtgagtg aagaccttca
    10801 tcaagtcaag gcgtcctacg cttttccgca gggttgcgat acaatccccg catgaacaga
    10861 tgtctgtcga tcaggcctct ttccaggacc ggcgcccggc tgcccgcgac ggcacctctc
    10921 ccttccaagc gatgtactcg atgagcaacc ggctgttggt tcgttttggc accagttcat
    10981 gggcctatga aggctggcgg aaccaaatct atcaccgtcc ctatgcgtcc agccggttct
    11041 ccaaggatag tctggctgag tatgcgacgt atccaacagg cgggacgccg ctctttcgca
    11101 ccgtcgggat cgaccattcc ttttatcgac cggccagcgc cacgcagctc gcgcattacg
    11161 caagtcaggt tccggctgat ttccactttt gctcgaaggt gtgggaagaa ctgacggttc
    11221 ccgcctatgc cgatcttcca cgatatggcg ccaaggccgg aaaacccaac cctcgatttc
    11281 tcgatagcca tctctttcgc gagatggtgt tgcagccgtt tctcgaagcc cttcccggtc
    11341 acattggccc cttcatcttt gagtttcagc gaaccggaat ggaccccgtc gtcttcctcg
    11401 atgcccttga ccgtttcctg gccgacctgc cagccgggtt tccctacgcc gtggagatcc
    11461 gaaatcctgc cgtcctgagc cctcgttatc gggacattct cctcgcacac caggtcgcgc
    11521 acacctataa ccactggacc ggaatgccgc cgctgctgac ccagcatcgt ctcctcaacg
    11581 aacggttcac agccccattt gtcgtgatgc gcctcctcac tccgctcggt ctcccctacg
    11641 ccgacgccgt ggagcggtat gccccgtaca atcgcatcgt caccccgcag ccgcaaatgc
    11701 gccgggaggc cgcctcgctg attcactccg cttcctccca aggcgtgacg ccttacgtat
    11761 tggtcaataa ccgctcggaa ggcaatgcac cgttgaccat ccaagcgatc gtcgagatgg
    11821 tgacggcgct tccggactcc cccgcattgc tgccgtctcc ctcgcgtgat agcatgatct
    11881 gacgtgagca ccgccctgaa cctgcacgca ccagaatccg acaggaaaga ggaggtatct
    11941 cccatgaccc cgctgctgtc gatgttccga agcggcgtcc tcttcgcggt cgccgttcac
    12001 tgcctggccg tggccattcc cgtccaggcc gcctcagacc actcccgtgt cgacatcacc
    12061 ggcgattatc gttacgccta tcaccagccc gagacggcat cggaggccaa gcagcatgcc
    12121 tgcagggaag cgctgcatca ggccatcagt acctcgacgg ccgtgcgtga gcataccgcc
    12181 tcgatcgtcg actcgaaact gttccacacg ctcgtccata ccctggctgc tcagcatgtc
    12241 accgatcaac agatccttca gcaaagcgaa cagggccgca cggtgtactg caagatcaag
    12301 gcagtcttcc aagcggacgc cgtggagcga gtcctcctgg ctcagaccac gagcggatca
    12361 gaacaggccc tggatcagaa tcgggcgctg agaattctgg ccgcgcatga ggagccggac
    12421 gggatgctgg tgatcaccta ccaggcgatc aagcgtctcg attggcttgg cacggcctat
    12481 cagggaggat tgcgcgaatc agccgatgtg atggtggatt tttacgacga gcaagggatc
    12541 ctggtgcgca gcagccgcca ccccgccaga aaaacggcga cgggtgatga cgtgatgaac
    12601 cccggcgagg tggggaccct caaagttgcg aaaccgctga atgggaaaac cttccgtgtg
    12661 tgggtcgtga agtaaccccc aaaatgcagg aagcaaaaca ggatccccgg ttttgcttcc
    12721 cgtttcgcga tacccctgaa tcagcggcac gaattcgtta gcgatcggcg ccggacgacg
    12781 gcatattgat gccgactcgt tccaatccca tgatgaccgt cagggctgac ggcgcgtgct
    12841 gaacaatttc cttctgcacc tcgatccggc gaagatcaac aaactccggc gcggttagtc
    12901 ccaacgattc ccgataggcc cggtcggcga tcccgcgctg cttctcagcc ttttcacgcg
    12961 cctcttcggc tttttgaaac tccaccatcg taatcttgcg ctgttcctgc acgatagttt
    13021 gcgtggtctg ttcgaccacg cccttcggcg gcagaatgct gccgacgacc acgcgattga
    13081 gtcgaatcgg catattctgt ttatcgatga gcttgttctg aacctctcgc gcaatggcat
    13141 cctgcaactt tgttctggtc gtcggatcgg tcgtcagctg aaagagcgga taccgttgca
    13201 cttcttcccg cacgaaggtg cggaacgctt ctttcacatt gttctggtac caggtggtgc
    13261 catatcgccc gatgagttca ggcgtcctcc cttcaacgac gttggcaatc aggaaggcat
    13321 cgaaggatac cggagcattt tctgcggaga tgatatcgaa atgttcggaa tactgtaatg
    13381 gacggatatc cacttcgatc accttcgtcg taggtgccac ccacacccgt ccggtcttgg
    13441 tcggcgccgg atccactccg ccgtggccga agaagaatgg ctgttccacc atgacgccct
    13501 catgaccggc ctcgatcgcc acacagcctg aagtcaccag catcactccc atgattccga
    13561 gcacggacca ggctccctgt ctcataggtt cctccaatga aaaggtccga ccaccgagcc
    13621 gaattccctg agcgggggaa ctcacggcca cgtgttgtcg tgcggatacc ttacgaaccg
    13681 cgtgggcggt tgtcaaacca ggcttgagga tgaagagaaa acgagaaaag tgaggagaag
    13741 cgcaaccaaa cggacggcaa gtcggccggc tagtaaattc cggcctcgcg ctggagccac
    13801 cggacgtatc gaatgatctg ttccacatcg gccggcgtca ccccctcgat cttcggcata
    13861 ttgccgaact cccaatggtg ggctcgaaca ccgttttccg ccgcgcgctg gaacgccata
    13921 tcggcatgat ggttcggttc atagatcttg tggaccaacg gaggtccctg cttggtgccc
    13981 acaccacgaa ccccgtggca gacggcacaa ttggcactga acttcgcctc gccgacctgc
    14041 acatcggccg gtgtggcgcc tccggcaggc ttgcctgcat cctgattggt ggcgttcgat
    14101 tcgcaggcag gcagggccgc gacaaggacg accaagccta ttcccgccat ccaggctttc
    14161 atccggatgt gtgtggatct attcaagttc atcggggtag ctccggcgga gtcgtcgagg
    14221 acggcttgga ttgtgaagaa tgcaattgct cgcgcaacat ctgcgtggtg cgattcaacg
    14281 tcaaccgccg gtagagccgg gcgccgatag gcgcaatgat caccagcgcg atgatcagat
    14341 acatgaaaaa ttcttcaggc gtcatgggcc catctctcct gactcatagg caaccgcacc
    14401 aaccgttcgc tagagtatcg cataggggac cggatccaca actcccgcta gacggaatcc
    14461 ttcccgccga atccggcaac tgtcacaccg gccgcaggcg agcccctcga cgccaggatc
    14521 gtaacaacta tgcgtcaact cgaagggaac gttgagcgcc tgcccgcgcc gcacgatgtc
    14581 cgccttggac atcatcagca ggggcgctcg aacctcggcc ccatgcccct caacccccat
    14641 cttggttcca agccgggcca cggtttcaaa cgcgcgaata aaatcggggc ggcaatccgg
    14701 gtatcccgaa tagtccagga cgtttgctcc gaagtagatg gccgaggcct tcactgtctc
    14761 cgcataggcc aatgccagcg agaggaagat ggtattgcgg gcgggaacat aggtcacagg
    14821 gatttcgctt gcccgtgctt ctggtgggcg atccttaggc acgtccacat ccgccgtgag
    14881 ggccgaccct ccgatggccc gtagatcaac cgtgaccacc acgtggccgg cagtgcctag
    14941 cgccgcagca acggcacggg ctcgttcgac ctccacctgg tgacgctgtc cataggaaac
    15001 cgttagacag tgaatgctat acccgtcctg ctgcgcgatg gcggccgtca cggtggaatc
    15061 caagccaccg ctcaagagga ccacggcctt cggctggaga ggagtcatag acgcggggaa
    15121 gaataagggg gaatcgcgaa gccggtcaca ggtgagcgag tgaccggctt ctcatatcga
    15181 tgcattccgc atacatgcgg tggaaacgga aggggttgcc cgattcggtt agtcccgatc
    15241 ctcttccttc tcaattttct cgaggagttc ctggcgagac tggcattgca cgcacaactt
    15301 ggcgaagggc accgcctcca aacgcttctc actgacttcg atgccacatt ctgcgcagat
    15361 accgtaggtc ccttcgctga gccgcgtcag cgcttcgtcg atggcctggc gcttccggtt
    15421 ccgcatttcc atcagagaaa tgccgagctc gcgatccaga tccatgagcg cctgatcacc
    15481 cacatcgcgg gccgactcca gccggcgctg ctgatcttcg gtcagcgact ggccgaggtt
    15541 gccctcgatc tcgcgcatga tttcctggcg cttgccaagc agcatccgct gcaggacttc
    15601 gcgccggcgc tgacgttcct ctttctcttt cggcgtttcc ttaagcgtca acgcggccac
    15661 cacgcgtgcg gccacgctgt cttcgtcttt ttcagtcata gttgttacag gagccggtcg
    15721 ctctttcttc acgggttttt caggagcgat ggcttttgct ttcgtcgttg gtgttttttt
    15781 cttcgcgccg actttcgtcg ccatagagtc tcatgccctt tcgtgatgag tgcaaggcga
    15841 aaaa
//
LOCUS       DAOQDS010000081        15765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1567_length_15765_cov_23.022979, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000081 DAOQDS010000000
VERSION     DAOQDS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15765
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1567_length_15765_cov_23.022979"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(507..869)
                     /locus_tag="PK589_15875"
     CDS             complement(507..869)
                     /locus_tag="PK589_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21139.1"
                     /translation="MSLLLDRILAADFEVPDVELLRSPTVVQTNNVAQYLHGTNRKDP
                     DDLTQDFPNIAPPWPNFFVEFAMPSTICEVTPEGSQMMQWEGPKTFGLHWMAGRRGSY
                     DFGLQQSLFKTLSESTNP"
     gene            complement(1165..1914)
                     /locus_tag="PK589_15880"
     CDS             complement(1165..1914)
                     /locus_tag="PK589_15880"
                     /inference="COORDINATES: protein motif:HMM:NF009385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ21140.1"
                     /translation="MNNHTLKGKVAGAGAKNLGGLLSREFAKGGAKVVVHYNSDTTKP
                     NADDTVKAIKDIGGEAYAVQGDLTKPTNIAALFKSAKDRFGGIDIAVNNVGKVLGKPI
                     VDTTEAEFDSMFDINAKAAYFFIKEAGTNLNNNGKIITIVTSLLAAFTDGYSTYAGGK
                     APVEHFTRAAAKEFGPRGISVTAVGPGPMDTPFFYGQEMSERVAFHKSQALNGNLTKI
                     EDIVPIVRFLATEGWWVTDQTIFANGGYTTR"
     gene            complement(1995..2447)
                     /locus_tag="PK589_15885"
     CDS             complement(1995..2447)
                     /locus_tag="PK589_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNJ21141.1"
                     /translation="MSGKTKPTDPSAKMYYNDRWSKGARANTNGVKQAGIGCLLFVGG
                     AGSLEVKPGVQSVDTPEFLKDWKQGALATREVLNLLRKELSLDWSFLSPSADLSPGQR
                     TGNFRLGKDQLLVDAKGQSRISVEDYALAMIDEAEAPHHIRQRFTVGY"
     gene            complement(2604..3071)
                     /locus_tag="PK589_15890"
     CDS             complement(2604..3071)
                     /locus_tag="PK589_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21142.1"
                     /translation="MLIKELLRLQATQTYLRAIVGGDFNFEHHSPEYQELLVAGLRDT
                     HMIASPRTDLYSSDPNQNTIVHQEELTMPPSLRQAMTNLPEAEQQRMAEGYQDGMGQA
                     RRIDYLFHMGKGPHLPNECLRQTLLGESAALSTPSGSDHFGVLNTYILDPSQR"
     gene            complement(3138..3524)
                     /locus_tag="PK589_15895"
     CDS             complement(3138..3524)
                     /locus_tag="PK589_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21143.1"
                     /translation="MTGRLSEGTDEFHSPHGLDGPDHFYTFALAQRTRMEIAVAANTS
                     HWSDAVGLTRAAWQPALYLLSADGAMLKEGHVFRAGVTSLFPAEFEPGTYYLVVDSSI
                     REWSRGDGYYRLYVGFNEEVMGPLVP"
     gene            complement(4000..4416)
                     /locus_tag="PK589_15900"
     CDS             complement(4000..4416)
                     /locus_tag="PK589_15900"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNJ21144.1"
                     /translation="MLTTIEKVLLLQGVDILVDTETENLAKIAAIAREVRFEEGATIF
                     EEGEPSNAFYIVLEGTVSVRKGSKEIGLVKDKETVGWVNLLEQTPRTVTLVALTGTTF
                     LKIEREGLFDLLADHFDIVRGILKRLSRIINQLLDR"
     gene            complement(4422..7190)
                     /locus_tag="PK589_15905"
     CDS             complement(4422..7190)
                     /locus_tag="PK589_15905"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNJ21145.1"
                     /translation="MNFVPILQKLVDIRSGEYRKTVLMFLYIFLLLLSFVIIKAVRDA
                     LFLVRLGPEQLPYIYILVAVIVGVVAWIHAKLSARLRLDVLIRGTLLIVVANLLIFWW
                     LFRFGWPSLYYVFYIWAAAIGLLTMSQYWLLANSVFNAREAKRLFGLIGAGSILGGLV
                     GGVVTARAVTWIGTEGLLIIAATGYLLCGGLALIVGRAAVPAARPSGNASGMSAQAEA
                     AAHSEESILTTIRNSPYLITIAGLIGIGEIVVMLVDYQFKVIATQLFPSKNDLTGFMG
                     IFSALVSASSFLVQVFFTGRILRSLGLGLALLCLPVGLLFGSVAIAVVPGLISAVMAK
                     LTEGSLRFSINKASLELLYLPLPWAIKAKTKPFIDPASERVAGGIGGLILIVATSILS
                     LSTAQISFVTIGLLSLWVPLALAGQRQYVDSLRLALERRRLDLTFPILNLSDRRTVDL
                     LIRHLLDSDDAGTCYVLRLLEQADVKPYIPAFKQLLTSRSPEVRAAVIRLLSEIREPS
                     LVPALTDVLGDQEMEILVGAVRFLDAHPGSSGAKLEEWMASTDPRLAAAAIVGLLNDP
                     AQRLGARAEQALRSMIQAHGPQGEIRRREAAKALGWIHPEATASDALIRLLQDDSIEV
                     VITAIKSAGRIQRREFVSPLVALLVRKETKGEAKTALVGYGSKILGGLADLLEDDNER
                     MELRRTLPSLLAEIGGERAIAILLHNLNQPDRLLRYQVVKALNRIRAHDPNLEPEPEL
                     IDASLTSELRHHYELLLLFHSPSLVEDSQGGRLLKKALQERLDHQLEMVFRLLALRFP
                     VSDIRDAYAGLTVDDARLRAIATEFMDNLLPSHLKRYLIPIIDNWPSDAVVEVGRQFV
                     SINGSTREELILYLLKGTDLWLKTCAIYLAGEWKLSSLLSELRQAADDSGHLIRETVH
                     LALEQLND"
     gene            complement(7212..9002)
                     /locus_tag="PK589_15910"
     CDS             complement(7212..9002)
                     /locus_tag="PK589_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21146.1"
                     /translation="MRDWNWRRRISAFSCDGVAAWFLCALALSVIGCYNRVVLQEPVV
                     WEVNDRRPISEPQEETIGQWALWDGTDKMVFYRIGQLFNLGRSMRDLGAWVGLADPIE
                     AQNVNAFDEVPNSTWFTNRQFFSRRSEEELTAGPTTDRGAPDTAGPWTAIAAKASLGS
                     TPGFVIRDQKNDVYLIKFDPPLYPEMTTASEMITPRFLHAAGYNVPEHYLVYFDPAKI
                     RIDPKAKFRGKYHVPRPMQEQDITAILDRIPHRPDGTIRAMASKYLADVPKGPFLYEG
                     KRPDDPNDRILHENRRELRGFRILAAFVNHTDTKAANALDTYDPETRYLTHHLIDFSS
                     TLGADNADPQFPRFGNEYFFDFGTVGHSTIAVGSYVRPWEIPLEMHYPAVGYFESLYF
                     DPPRWRPTYPNPAFLRMTLRDAYWGAKIVTSFTDEDIDAIVHTGRYTDARAERYVADV
                     LKARRDKIGRYYFNLINPLDRFAIDTKFDEQVLVFENLALSLGYAPAPHALYRYSISK
                     YVPNWFDTSVIQETLVQSPKIRLDKAFLEAWGKHTADGRATEEERPIAAVRIQTSYDD
                     GSHWGKRVVVYLREHPGTRRMTIVGLERET"
     gene            complement(9062..10426)
                     /locus_tag="PK589_15915"
     CDS             complement(9062..10426)
                     /locus_tag="PK589_15915"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNJ21147.1"
                     /translation="MARILSALLLSVLCAINGCTSAQVTQTHASQPNAFQAEGKKQEQ
                     SQEARSPEERLRKEKPQEERDEEDTVLSAIAAAPEYLVKGLAYPFKKFGIYYERTDLM
                     NRLMDLFYNDERTGGVFPRMAVGGALSSGIGFTAFHQDLFHQKKEVRARYLYATRGNQ
                     AADFAYRDPSLFGSKFMLDLDVFWLNFDNGFFFPGGNRAAENGKTKYDLNQLSQNARL
                     SYMVAPNLKASLLGRILFTDAGPSSRTPTTPPTVPGIDTYMQGAAVEPSLTYDSRDNP
                     FRPSTGLYIDTGFTYTNQLNGDQFRYMGYWVDVQRYIPVFRGDRVLVIRAYLSKLDSL
                     DGKSIPFYELNLLDLNNGLRGFDRGRWQDKGALLYNLEWRYPVWKDVEGTIFLDLGQV
                     FGDYKDLQPKLFQYSAGGGFRFVTSRSFAFRVQLAASNDGLLALFRGDLEFVRHRGTV
                     LGGF"
     gene            complement(10513..10890)
                     /locus_tag="PK589_15920"
     CDS             complement(10513..10890)
                     /locus_tag="PK589_15920"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNJ21148.1"
                     /translation="MTKFAVGRERPNEGRGTHSFDPFHEFDRSFPSSHTARAFAVASV
                     FADRYEQPIPLIAYGTAAFISVSRVYLNEHFASDVVAGAVLGFVIGKVLSWRHKDENR
                     RWTVLPYVPDARGGLGMTFSCSF"
     gene            10922..11299
                     /locus_tag="PK589_15925"
     CDS             10922..11299
                     /locus_tag="PK589_15925"
                     /inference="COORDINATES: protein motif:HMM:NF014870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNJ21149.1"
                     /translation="MGECSLGSILVAKSERGICALFLGDDPDALARELQDQFPRATLI
                     GGDADFDQLVAKIVGFVEAPSLGLDLPLDVRSTAFQGRVWQALRRIPAGLRMSYREIA
                     EHLARKCLWKLIVSGRHRGMGRD"
     gene            complement(11561..12925)
                     /locus_tag="PK589_15930"
     CDS             complement(11561..12925)
                     /locus_tag="PK589_15930"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ21150.1"
                     /translation="MSLVSLLRATFQGLRDDERTQGTLEVGEQLSSKRNVRWLWTGEL
                     ISQLGDGLNRPAILWFVYDLTGSAVKMTFIGVLQTIPPLVLGPVIGVYLDRLSADWKL
                     RVMISLDLARGALLAWLATLSILGMLTLPTLYTFVFLIALAAMPYGPALNSTIPSLVQ
                     ASRLTAANAAIQSTATLGLLCGPAISGATVAFLGVGYVLYANAATFLLAAFSKMPIRV
                     NSEAPSPSRSGTSSGWINELAAGFRLVFVEQRIIFRVALLGALFTLASTGFIFLLPII
                     TKNILSGGPMFLGVIWSALGLGMIFMSVLLMSSARRTLIDHLLLISAGPVIEGLAIVG
                     LAAVDSLVAALGLVIVIGAGTALVMPMITAFIQATAPQETRARALTIFGTITMVSAMA
                     GMTAFSWAADHLGSRIALVVLGVVQMMAGVIGIMFTQSSEFQTNLASRSNDHPSSKSD
                     TGIT"
     gene            complement(13343..14038)
                     /locus_tag="PK589_15935"
     CDS             complement(13343..14038)
                     /locus_tag="PK589_15935"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNJ21151.1"
                     /translation="MRNDIPLKCCWANLRLGRGRQSDNPSGIALPHFIGICLVVFVLG
                     GCVNLSKSYPEKHSYILEAVHSGEMRASIPGTVLKVRKFRVSPTSEGKELVYRTSDAR
                     YEGDFYNEWFVPPNAMLTQQIMNWLTSAGLFQYVMDSSGPLPATHMLEGTLTALHGDY
                     RATPARAVLAVQFLFLHEASAQGEVLWHREYRKEVDIMEQKPEALVSGWNEALRLILS
                     ALDEDLTRTLRRQ"
     gene            complement(13968..14999)
                     /locus_tag="PK589_15940"
     CDS             complement(13968..14999)
                     /locus_tag="PK589_15940"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNJ21152.1"
                     /translation="MSAKANYHKIGVFIVLGLVTLIIAIVALSGGKWFKNVVYWESYF
                     DESVQGLAVGSPIKYRGVQVGTVEAIGFVGDVYGAELGKEDLLRYGRYILVRGSVADL
                     SPHLTEEEKKAQHGGRITAGLRVRLASQGVTGVVYLEADYLDAQQYPTLDVPWKPQAV
                     YLPSAPSTVSVLGAALHTIARDLEKADIHTITRNLDSLVLEVTKLVKDTNEQALSSRT
                     GRVLAEFQETARQTRRLVESPEIRAMVSDAAGTVQGARQLIADLSEASKQIKIASEQF
                     PGVFARLERTVQRIDRLVSNKSQDIEDTVENLRVMSENLREITDNAKRYPAQVLLGEP
                     PPRAGATKR"
     gene            complement(14996..15760)
                     /locus_tag="PK589_15945"
     CDS             complement(14996..15760)
                     /locus_tag="PK589_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ21153.1"
                     /translation="MQHFTAAYDGSVVIDDISFEIYRGEVFAILGGSGSGKSTLLKHM
                     IGLYQPAAGHIVIGGKDIVTAEGEERTSILKSIGVTYQMGALFGSMTLLENVRLPLEE
                     YTALPSDAMDLIARMKLKLVGLEPFLDYMPSELSGGMQKRAAIARAMALDPHILFLDE
                     PSAGLDPITSAELDRLILDLARNLAITFVIVTHELSSIDTIADRVIMLDKVVKKIVAT
                     GRPQELREHATNPWVRQFLNPGVSAETAGKGTGGER"
BASE COUNT         3509 a         4554 c         4276 g         3426 t
ORIGIN      
        1 tggctcaaac tgtggccccc actttcagtt cgtgatagtc tatcttatca cgtctgtgaa
       61 ggcgacggtc tagagacatg acgggcgaag gagtggcagt ctagcagacc gcacaatacc
      121 tcaacggtcg tggatgtcat gggaggccgc tgtaccaaca tcgcatgcca gatgagacac
      181 gcagtccttc cacgctgact actgcggcat tggcaataca tggcgaagcc gtgaaccaca
      241 gcatgatcat gagacaacac ttgacccaca ttttcattgt gcccgcttta ttcagatgat
      301 gaaaatcatt gtcctgttgc ccgagttcgc taccgcctcg gacttaggcc cgcaactatt
      361 tctcccgtct aacgtcccag agtaaacggg gagggcaatg tggcaaagtg atttcccttt
      421 actcgactgt aagaaacaac caagaagaga tgaacgtgcc cgaaccaagc ggcttgttct
      481 gcagcttcta gatattttga acgtatctag ggattggtgg actcagaaag tgtcttgaat
      541 agcgattgct gcaaaccaaa gtcgtaagaa cctcgtctcc ctgccatcca atgcaaccca
      601 aatgtttttg ggccttccca ttgcatcatt tgcgatcctt ccggagttac ctcacaaata
      661 gtcgaaggca tggcgaattc cacgaagaaa ttcggccaag gaggtgcaat attaggaaag
      721 tcctgggtaa gatcgtctgg atctttacga ttggtaccgt gaagatactg ggcaacattg
      781 tttgtctgaa caactgtagg tgaccttagc aactcgacat ctggaacctc gaaatcggct
      841 gcaagtatgc gatcgagaag aaggctcatg taatagccct attcggaaaa ccgctttaaa
      901 cccctcacca cttcaatttg ccccttcttg aaacaatctg aatagaactt ggctggcccg
      961 cttaagcttc cctgcgcgca tcgatcgtgc acagcttcac cgtgcgcgtt ctccgagcaa
     1021 agagtacgac ctggccgtcc tgcttctcct ccgccacaca gtcaaatgct gcttcaccat
     1081 ttggcgcaac tcatcactct tgcccgcagt gtgacgcgct tatcgctgct gaagccgact
     1141 gttagggttt tgacaccaat cgaatcatcg agtggtgtat ccaccattgg cgaatatcgt
     1201 ttgatctgta acccaccaac cctcggtagc aaggaatctt acgatcggca caatgtcctc
     1261 aatcttggtg agattgccgt tcaaggcctg cgacttgtga aaagccactc gttcagacat
     1321 ttcctggccg tagaaaaacg gcgtgtccat cgggccggga ccgaccgcgg tcacggaaat
     1381 accgcgcgga ccgaactcct tggccgctgc gcgggtaaag tgttccaccg gcgctttgcc
     1441 gccggcgtag gtcgaatagc cgtcggtgaa ggccgcgagc agtgaggtca cgatggtgat
     1501 gatcttcccg ttgttgttga gatttgttcc ggcttccttg atgaagaaat aagcggcttt
     1561 ggcgttgatg tcgaacatcg agtcgaactc tgcttcggtg gtgtcgacga tgggcttgcc
     1621 cagcaccttg ccgacgttgt tgacagcgat atcgatgccg ccgaagcggt ccttggcgga
     1681 tttgaacaat gcggcgatat tggtgggctt ggtcaagtcg ccctggacgg cgtaggcctc
     1741 gccgccgatg tctttgatgg ctttcaccgt gtcgtcggcg ttcggcttcg tggtatcgct
     1801 gttgtagtgc accacgacct tggctccacc tttggcgaac tcgcggctga ggagaccgcc
     1861 cagattcttg gcgccggcgc cagctacttt ccctttcagc gtatggttgt tcataatgtc
     1921 tctccttccc tatcaagtcg gttgtggcgc gaatcagaac gtgctcgcgc gcagaggaat
     1981 ttccacatct caattcaata acccaccgta aaccgttggc gaatatgatg aggcgcctcg
     2041 gcctcgtcaa tcatagctaa ggcataatcc tcgaccgaga tgcggctctg ccctttcgca
     2101 tccaccagca actgatcttt cccaagccgg aagtttcctg ttcgttggcc aggggacaaa
     2161 tcagcggaag gtgagagaaa ggaccaatcc aagctgagtt ccttgcgcag cagattcaag
     2221 acttcccggg tggcgagggc gccctgcttc cagtccttca gaaactcagg cgtatcgacg
     2281 gattgaaccc caggtttcac ttctaagcta ccggcgccgc caacgaagag caagcatcca
     2341 ataccggcct gcttcacacc attggtattg gcccgtgcac cctttgacca tcggtcgttg
     2401 tagtacatct tggcggaggg atcagtcggt ttcgttttgc cgctcacgat acgactcagc
     2461 ggtgtccact gtcattgtcc ttcgtcttgt agaccttcat cgatggccgc tcgcctgcca
     2521 ttcacacagg aatccacagc agcctccggc tctgcgatcc tcggccagag aatcgctgtc
     2581 cccagccctc cagctagaaa tgatcagcgc tgcgacgggt caagaatgta cgtattcaac
     2641 acgccgaagt gatcggaccc ggacggagta gagagagccg cagattctcc aagcagcgtc
     2701 tgccgcagac actcgttagg taagtgtggg cccttgccca tgtgaaacag ataatcgatc
     2761 cgtctcgctt gtcccatacc gtcctgataa ccttcggcca tcctctgctg ttccgcttct
     2821 ggaagattcg tcatggcttg gcgaagggag ggtggcatgg tgagttcttc ctggtggacg
     2881 atcgtgttct gattcgggtc ggagctgtac aggtcagtgc gggggcttgc gatcatgtgc
     2941 gtgtctctca ggcctgcaac cagcaactcc tgatactccg gggagtggtg ttcaaagttg
     3001 aagtcccctc cgacgatcgc tctcaaatag gtttgcgttg cttgaagcct caatagttcc
     3061 tttatcagca ctcgagtctc cttgatccgt ctgttttggc tctccaacgc ggcaacaaag
     3121 tctcgcacgc cagcaaatca tgggactagt gggcccataa cttcctcgtt gaacccgacg
     3181 tagaggcgat agtacccgtc cccccgcgac cattctctta tggaactgtc caccacaaga
     3241 taatacgtcc caggctcaaa ttccgcgggg aacaaagagg tgacaccggc acggaagacg
     3301 tgtccctcct tgagcatcgc cccatcggca gagaggagat acaatgccgg ttgccaggct
     3361 gcccgggtga gccccaccgc atcggaccag tgcgaagtat tggctgccac cgctatctcc
     3421 atgcgagtcc gctgagccaa ggcgaaggtg tagaagtgat cgggaccgtc taacccatgg
     3481 ggagagtgga actcgtcggt tccctcactt aaacgtccgg tcaccacaat gctttcggtc
     3541 aatctatccg catgggtcaa ggtatttccg ccgcgcaggg gcgcatcttt caaaggctgt
     3601 gtgccacagg tggcccaggt gagttccctc tcatgccggc tggccacttg gcagaccgtt
     3661 ttctgggatg gacaggcctg cccggctcca tgatccgtcg cacgttcata cccatatctt
     3721 ggctcaatgg gagttaccag agaaggctcc gagccattgg tcgtctgaga gtcgtcaatg
     3781 cgaaagaacg tcgcacccga caggaggcaa ttaacaagcg cggtgaattt ggcttgtcgt
     3841 tccagattag cccaggggca ctcttgcgag cttggttcac atgcaagctc cgaacggtat
     3901 tccactgtat gtggaaggtt tcttactctt acctcacaac caagcatcga gctcgcgatg
     3961 acgattaagg cacacgacag aagataaatc ggactcatct catctgtcca aaagttgatt
     4021 gataatccgc gacagccgct tgaggatgcc gcgaacaatg tcgaaatgat cagcgagcag
     4081 gtcaaacaag ccttctcgct cgatcttgag gaacgtggtg ccggttaacg ccaccagagt
     4141 cacggttctc ggggtctgct caagcaagtt gacccatccg accgtctcct tgtccttaac
     4201 gagtcctatc tccttcgaac ctttccttac cgagaccgtt ccctccagca caatgtagaa
     4261 cgcattgctt ggctcgccct cctcaaaaat cgtcgccccc tcttcgaaac ggacttcccg
     4321 ggcaatggcc gcgatcttgg ccaggttctc agtttccgta tccactaaaa tatcgacacc
     4381 ttgtaacagc aggacttttt caatcgtggt gagcattcgg tctagtcgtt aagttgttcg
     4441 agcgccaggt gaaccgtctc cctgatcaag tgtcctgagt catcagcggc ttgccgcagt
     4501 tcgctgagca gagatgagag cttccattcc cctgctaagt aaatggcaca tgtctttaac
     4561 cagagatccg ttcccttaag caagtacaag ataagttctt cccgtgtgga cccattgatc
     4621 gagacgaatt gcctgccgac ttccaccacg gcgtccgacg gccagttgtc gatgatcgga
     4681 atcagatatc gcttcagatg ggatggaaga aggttgtcca tgaactccgt ggctattgcc
     4741 cgaaggcggg cgtcatccac agtgagaccc gcataggcgt cccggatgtc cgacacagga
     4801 aatcgaagcg ccagaagacg gaacaccatt tctagctgat ggtccaaccg ttcctgtagc
     4861 gcctttttga ggagtcgccc cccctggcta tcctcgacca acgaaggact gtgaaagagg
     4921 agcagcagct cataatggtg ccgtagttcg ctggtcaggc tggcgtcgat gagttccggc
     4981 tccggctcca aattcggatc atgcgcgcga attctgttga gcgccttcac gacttgataa
     5041 cggagcaatc ggtcgggctg attgagattg tgcaggagaa tggcgattgc cctttcccct
     5101 cctatctcgg caagcagcga gggaagcgtt cgtcgcagct ccattcgttc gttgtcatct
     5161 tcgagcaaat cggcaagacc ccccagaatt ttggagccat acccgactag ggcggtcttg
     5221 gcctcgcctt tcgtctcctt tctcaccagc aaagcgacaa gcggagaaac aaattctctg
     5281 cgctgtatcc tgccggcgct cttgattgcg gtgatcacga cctcaattga gtcatcctgt
     5341 aacagccgta tcagggcatc cgaagcagtc gcctcgggat gaatccaacc caaagctttg
     5401 gcggcttccc ggcgacgaat ctcgccttga gggccgtggg cttgaatcat cgagcggagc
     5461 gcctgttcag ccctcgcacc caacctttgc gccgggtcgt tcaggagacc gacgatcgcc
     5521 gcggccgcga gccgcggatc ggtgctcgcc atccattctt cgagcttcgc gcccgaagag
     5581 ccgggatgtg catcaagaaa gcgcacggcg ccgacaagaa tctccatttc ctgatcccca
     5641 agcacgtctg tcagcgccgg cacgagagag ggttccctga tttcgctgag gaggcgaatc
     5701 accgcggccc ggacctcagg agaccgactg gtgagcaatt gtttgaaggc cgggatgtac
     5761 ggcttgacat ccgcctgctc caggagcctg agaacgtagc acgtcccggc atcgtccgaa
     5821 tcaaggaggt ggcgtatcaa cagatcgacg gtccgtcgat ctgagagatt cagaatcggg
     5881 aatgtcagat cgagtcgcct ccgctccaag gcgaggcgca aagaatcgac gtattgtctc
     5941 tggccggcca gggccaatgg gacccagaga ctgagcagtc cgatggtcac gaagctgatc
     6001 tgtgcagttg acagcgagag gatgctggtg gcgacgatca gaatgaggcc cccgatgcct
     6061 cctgcgacac gctcggaagc tgggtcgata aatggcttgg ttttggcctt tattgcccat
     6121 ggcagcggca ggtacaacag ctccagactc gctttgttga tcgagaatcg caaactgccc
     6181 tcggtgagct tcgccatgac tgccgagatg agccccggga ccacggcaat ggcgacagaa
     6241 ccgaagagta agccgacagg gaggcacagc agggccaaac caagccccag gctcctcagt
     6301 attcttccgg tgaagaacac ttgcacgagg aacgacgagg cgctgaccaa tgccgaaaag
     6361 attcccatga agcccgttag gtcgttcttg gacggaaata actgcgtggc gatcacctta
     6421 aattgataat cgacgagcat cacgacgatc tctcctatcc cgatcaaacc ggcaatcgtg
     6481 atgagatacg gagagttcct gatcgtcgtc aggatcgatt cttcggaatg agcggccgct
     6541 tccgcttgag cggacattcc ggatgcgtta ccggaaggac gagccgccgg cacagcagca
     6601 cgacccacga tcaaggccaa accgccgcac aagaggtacc ccgtcgcggc gataatcaac
     6661 aggccctcgg tcccgatcca agtcacggct ctcgcggtca cgactccccc gacgaggcct
     6721 ccgaggatgg atccggcacc gatgaggccg aagagtcgct tggcctctct cgcattgaag
     6781 acggaattgg caaggagcca atattgggac atcgtgagaa gaccgatggc ggctgcccag
     6841 atgtagaaca catagtaaag cgagggccag ccgaaccgaa aaagccacca gaagatgagc
     6901 aggttggcaa cgacaatgag cagcgtcccg cgaatcagca catcgaggcg aaggcgcgcc
     6961 gaaagcttcg catggatcca ggctaccact ccgacgatca cggccaccag aatgtaaata
     7021 taggggagct gctcagggcc cagcctgacc agaaagagtg catcgcgcac cgccttgata
     7081 atcacaaacg agagtaacag gagaaagata tagaggaaca tcagaacggt tttccgatat
     7141 tcaccgctcc tgatatcgac tagtttttgg aggattggga cgaagttcat cgagttcgcc
     7201 atgcgaacaa tctaggtttc ccgctccaga ccgacaatcg tcatgcgcct ggtccccgga
     7261 tgttctcgaa ggtacacgac gacacgcttg ccccagtgag atccatcatc atagctggtt
     7321 tgaattctca ccgccgcgat cggccgctcc tcctccgttg cgcggccatc ggcggtgtgt
     7381 tttccccagg cttctaggaa cgctttgtcc agacggatct tgggcgactg aaccagggtt
     7441 tcctggatca cggatgtgtc aaaccagttc ggcacgtatt tgctgatgga ataacggtaa
     7501 agcgcgtggg gagccggcgc atatccgagg gagagggcca gattctcgaa gaccagtacc
     7561 tgctcatcga acttggtgtc gatagcaaag cggtcgaggg ggttgatcaa gttaaaatag
     7621 tagcgtccga tcttatcgcg ccttgctttt aacacgtccg cgacgtagcg ttctgccctg
     7681 gcatccgtat accttccggt atggacaatc gcgtcaatat cctcgtcggt aaaggacgtc
     7741 acgatcttgg cgccccagta agcatcgcgc aatgtcatgc ggagaaaggc cgggttcgga
     7801 taggtgggcc gccaccgcgg cggatcgaaa tacaaggatt caaagtaccc tacggccgga
     7861 tagtgcatct ccaggggaat ttcccacggc ctgacgtagc tgccgacagc gattgttgaa
     7921 tgcccgacgg taccaaaatc aaagaaatac tcgttgccga accggggaaa ctgcggatcc
     7981 gcattgtcgg ctcctagggt cgaggaaaag tcgatgaggt gatgcgtgag atagcgtgtc
     8041 tcgggatcat atgtatcgag tgcattcgcc gctttggtat cggtatgatt cacgaaggcc
     8101 gcgaggatgc gaaagccccg caactcacgt cgattctcat gaaggatccg atcattggga
     8161 tcatcgggac gctttccttc gtagaggaac ggacctttcg gaacgtccgc caggtacttg
     8221 cttgccatgg cgcgaatggt cccgtccggc cggtgaggaa tccggtccaa aatggccgtg
     8281 atatcctgtt cctgcatcgg cctcgggaca tgatacttcc cacggaactt ggccttcggg
     8341 tctatcctga tcttggcggg atcgaagtag accagataat gctcgggtac gttatacccg
     8401 gccgcgtgca gaaaccgcgg agtgatcatt tcagaagccg tggtcatctc cggatagagg
     8461 ggaggatcaa atttgatcag atagacgtcg tttttctgat cccggatgac gaaccccggc
     8521 gtcgaaccga ggcttgcctt cgcggcaatc gccgtccacg gtcccgcggt atcgggagcg
     8581 ccgcgatccg tcgtcgggcc agccgtcagt tcctcttcgg agcgcctcga gaaaaactgg
     8641 cgattcgtaa accaggtgga gttcggcacc tcatcgaagg cattgacgtt ctgggcctcg
     8701 atcggatcgg ccagcccgac ccaagcgccc agatcccgca tcgatcgccc gaggttgaag
     8761 agttgaccga tccgatagaa caccatcttg tcggtaccat cccagagtgc ccactgaccg
     8821 atggtttcct cttgcggttc ggagatgggg cgccgatcat tgacttccca tacgaccggt
     8881 tcttgcaaaa caacccggtt atagcagccg atcacagaca gggcgagcgc gcaaagaaac
     8941 catgcggcga cgccatcaca agagaaagca ctgatccgcc gcctccagtt ccagtccctc
     9001 atctctcgcc tcctcggatc gccggatcta tctcgccttg cagctctgat tgcatcgcag
     9061 ttcagaaccc accgagcaca gtgccgcgat gccgaacaaa ctccaagtct ccccggaaca
     9121 aggccagcaa gccgtcattg cttgccgcca attggactcg aaaggcaaaa ctcctggacg
     9181 tgacaaatcg gaatcctcct ccggcggaat attgaaacaa cttgggttgc agatccttgt
     9241 agtcgccgaa tacctgccct agatcgagaa aaatcgtgcc ctcaacgtct ttccacacgg
     9301 gatagcgcca ttcgagattg tagaggagcg ctcccttgtc ctgccagcgt ccccgatcaa
     9361 atccccgcag tccgttattc agatcgagga gattcaattc atagaacggg atcgacttgc
     9421 cgtccaggga atccagtttg gagagatacg cccgaatcac caatacccgg tctccacgaa
     9481 agaccgggat gtatcgctgg acgtcgaccc aataccccat gtagcggaat tgatccccgt
     9541 tcaattggtt ggtgtaggtg aaccctgtgt cgatgtacaa accggtgctc ggacgaaatg
     9601 ggttatccct tgaatcatag gtaaggcttg gctctaccgc cgctccctgc atataggtat
     9661 cgatccctgg cacggtcggt ggggtcgtgg gagttcggct tgaaggccct gcgtccgtga
     9721 acaatatcct gcccagaagg gacgctttga ggttgggcgc caccatatag ctgagtctgg
     9781 cattctgcga caactggttc aggtcatact tcgttttgcc gttctccgcg gcccgattgc
     9841 ctcccgggaa aaaaaatccg ttgtcgaagt tcagccaaaa gacatcgaga tcgagcatga
     9901 atttcgagcc gaacaagctg ggatcccggt aggcgaagtc tgcagcctga ttgcctctgg
     9961 tggcgtacag gtaacgagcc cgcacctcct tcttctgatg gaagaggtct tgatggaacg
    10021 cggtgaagcc gatgccgctg ctcaacgccc cgcccacggc catgcgcggg aacactccac
    10081 cggtacgctc atcgttgtag aacaaatcca tgaggcggtt catcaggtcg gtgcgctcgt
    10141 aatagatccc gaacttcttg aatggataag ccaatccttt cacgaggtac tccggagcgg
    10201 ccgcgattgc ggagagcacc gtgtcttcct catcccgttc ctcttgcggc ttctcctttc
    10261 gaagccgctc ttctggagac cgcgcttctt gagactgctc ctgtttcttg ccttctgcct
    10321 gaaatgcgtt cggctgcgaa gcgtgcgtct gcgtgacctg tgcggaagta cagccattga
    10381 tcgcacacag gacgctgagg agaagcgcgg aaagtattct tgccatttgt ctaagaagct
    10441 cccatagccg gttccgcaat ctttacgatc gcatgtcgat cggcatcgcc aggccgttga
    10501 gccggcttcc agtcagaatg agcagctgaa cgtcatcccc aacccacccc tggcgtccgg
    10561 aacataaggg agcacggtcc accgccgatt ctcgtcctta tggcgccagc tcaggacctt
    10621 cccgatcaca aaacccaaga cggcacccgc gacgacatcc gaggcaaagt gctcattgag
    10681 atacacccgt gagacgctga tgaacgctgc cgtgccatag gcgatcaacg ggatcggttg
    10741 ctcgtagcga tcggcaaata cgctcgccac ggcaaatgcc ctcgcggtat gactcgaggg
    10801 aaaggatcga tcgaattcat gaaacggatc gaatgagtgc gtgccacgcc cctcgttcgg
    10861 ccgctcccgc cctaccgcaa acttggtcac gcctggcgcc cggacggaga tctggttcgc
    10921 cgtgggcgag tgctcgctgg ggtcgatcct ggtggccaag agcgagcgcg ggatttgcgc
    10981 cctttttctg ggcgacgatc cggacgcgct ggcgcgcgag ttacaagacc agtttcctcg
    11041 agccactctc atcggaggtg atgccgactt cgaccagctc gtcgcgaaaa tcgtcggctt
    11101 cgtcgaagcg ccgtcgctcg gtctcgatct gccgttggat gtgcggagca cggcgttcca
    11161 ggggcgagtg tggcaagcat tgcgaaggat tcctgcgggc ctgaggatga gctaccgcga
    11221 gatcgccgag catctggcgc gcaagtgtct ctggaaactt atcgtgtccg gccggcaccg
    11281 gggtatgggt cgtgactagc cgttcaatgg gtctttcggt acttgcggaa acaatcgctc
    11341 ttactgcccc gcgagcgcct gatagggcct gtcgcctctt gcgccagatt tcgccgcccg
    11401 gatgatgccg atcacctcac ggtcgtctca atcccctcta cttttcctct ggatgcagac
    11461 accgacaacc tgctgttccc acttcaatgt tcgtctctgt ctccgattac tggaaagttg
    11521 tgtcctattc agaacccgct ggagcagcga cgaaacgggg tcaagtgata ccggtgtccg
    11581 atttgcttga cggatgatca ttggaccgac tcgcgagatt tgtctggaac tccgaggatt
    11641 gcgtaaacat gatgccgatc acaccggcca tcatttgcac gactccaagc acaacgagtg
    11701 cgatacgact tcccagatga tcagctgccc agctgaaggc cgtcatgccc gccatggcgg
    11761 agaccatcgt gatagtgccg aagatcgtca acgctcttgc acgagtctcc tgaggagctg
    11821 ttgcctgaat aaaggccgtg atcatcggca tgacaagagc cgttccggca ccgatgacga
    11881 tcacgagacc aagcgccgcc accaagctgt caaccgctgc gagccccaca atggccaacc
    11941 cttcaatcac cggccccgcc gatatcagca acaaatgatc gatgagtgtt cgccgagcgc
    12001 tcgacattaa cagcactgac atgaatatca tgccgaggcc aagggccgac catatcaccc
    12061 caaggaacat cggccctcca gaaagaatgt tcttggtgat gatgggaagg agaaagatga
    12121 agcctgtcga tgcgagagtg aagagggcgc ctaacagggc aactcgaaaa atgattcgtt
    12181 gctcaacaaa cacaagccgg aatccagctg ccagttcatt gatccatcca ctggatgttc
    12241 cactccggct cgggctcggt gcttctgagt ttacgcgtat cggcatctta gagaaagcgg
    12301 cgagcagaaa ggtggcggca ttggcgtaca ggacgtaccc cacaccaagg aaggcaactg
    12361 tggcgccgct gatcgcgggt ccgcacaaca agccgagcgt tgccgtgctt tgtattgccg
    12421 catttgcagc ggtcaggcgc gaagcttgta ccagagaggg aatggtcgag ttcaaggccg
    12481 gcccatacgg cattgcagca agggcgatca gaaaaacgaa tgtgtacaat gtgggtaagg
    12541 tcagcatacc gagaatagag agggtcgcga gccacgccaa aagtgcacca cgcgcgagat
    12601 ccagtgagat catcacgcga agtttccagt cggcggacag acgatcgaga tacacaccga
    12661 taactgggcc gagaaccaag ggcgggatgg tctgaaggac accaatgaac gtcattttaa
    12721 cagcagaacc agtgagatcg tacacgaacc acaggatcgc cggcctattg aggccgtccc
    12781 cgagttggga aattagctcc ccagtccaaa gccaccgcac atttctcttc gagctcagct
    12841 gctcaccgac ctcaagagtt ccctgtgtcc gttcgtcatc ccggaggccc tgaaaagtcg
    12901 cgcgcagcaa gctcacgagg gacatagctt tacccaagag agttaggaag taagacctac
    12961 tattccggca taaccggggt atctatgaaa aggattctct cgctttggcg cacgacctcg
    13021 agcgcatagg aggccttctc tgaaaggtcc ttctacgttt cgcttcaact tcaatccgtc
    13081 gtggcctgct gccatgccga tccgcaaggt cgtgcctcct actactccct cacactgcct
    13141 tccgcaacaa gacctcgatt gaccatgccg tcactctgta gtgaagggat ggaacggttt
    13201 gttcttctcc cgggctgcga aagtcctccg ggctcgggag actcgtatcg atcacttgcc
    13261 gccagtccgt tggctgacct tcctggatcg tgaaggtaag atcgtcccaa ttacggccgt
    13321 aacggcttta tctgcccacg cgtcactgac gccggagtgt tcgagtgagg tcttcatcaa
    13381 gagcgctcaa gataagtcga agagcctcat tccaacccga cacgagtgcc tcgggcttct
    13441 gttccatgat gtccacttct ttccgatact ctcgatgcca cagaacttct ccttgagcgg
    13501 acgcctcatg gaggaagagg aattggacag ccagaacagc cctggcaggg gttgcacgat
    13561 aatctccatg cagtgccgtc agggtcccct caagcatatg cgtggcaggc aatggccccg
    13621 acgaatccat gacatactga aacaggccgg ccgatgtcag ccagttcatg atctgttggg
    13681 taagcattgc attgggcggg acaaaccact cgttgtaaaa gtctccttcg taacgtgcat
    13741 cgctcgtgcg atacaccaac tcctttcctt cagaggttgg cgagactcga aattttcgaa
    13801 ccttcaggac ggttccagga atggaggccc gcatctcgcc tgaatgtacc gcctccagaa
    13861 tataggagtg cttctccgga taactcttgc tgagattgac gcaaccgcca agtacgaaca
    13921 cgacaaggca gatgccgatg aaatgcggca gtgctatccc ggacgggtta tcgctttgtc
    13981 gcccccgccc gaggcggagg ttcgcccaac aacacttgag cgggatatcg tttcgcattg
    14041 tcggttattt cccgaagatt ctctgacatg actcgcagat tctccactgt gtcctcgata
    14101 tcttgactct tattcgacac gagcctgtct attcgctgca ccgtacgctc caaccgggcg
    14161 aaaacgccgg ggaattgttc ggatgcgatc ttgatttgtt tcgatgcctc tgacaaatcg
    14221 gcgatgagct gtctcgctcc ttgcaccgtg ccggccgcat cggaaaccat ggctctgatt
    14281 tcagggctct ccacaagccg gcgcgtttgt cgtgccgtct cttgaaactc cgccagcact
    14341 cgtcccgtcc ggctactcaa tgcctgctca tttgtgtctt tgaccaattt cgtcacttca
    14401 agcacaagac tatcgagatt tctggttatt gtgtggatgt cggctttttc caaatctctg
    14461 gctatggtat ggagagcggc gcccaatacg ctcaccgtgc tcggagcgga aggcaggtac
    14521 acagcttgag gtttccacgg cacgtctaat gtcggatact gctgcgcgtc caggtaatcc
    14581 gcctccaggt acaccactcc ggtcacgccc tgtgaagcca gccggacccg aagccccgct
    14641 gtaattctgc cgccatgctg cgcttttttt tcttcctccg tgagatgggg gctcagatca
    14701 gcaacagatc cacggaccaa gatatagcgc ccgtagcgca gcaggtcttc tttgccgagt
    14761 tctgccccat agacatcgcc cacgaaaccg atcgcttcga ccgttccgac ctgcacgcca
    14821 cgatacttga tcggcgatcc gaccgcgagg ccttggacgg attcgtcaaa atagctttcc
    14881 caatacacaa cgttcttgaa ccacttccct ccactcagcg cgacgatcgc gatgatcagt
    14941 gtgaccaatc cgagcacgat aaagacgccg atcttatgat agttggcctt ggcgctcatc
    15001 gctctccacc tgtgcctttt ccagcggtct cggccgacac gccaggattg agaaattgac
    15061 gcacccaggg attggtggca tgctcgcgta actcctgagg ccgtcctgtc gccacgatct
    15121 ttttgaccac cttgtccaac atgatcaccc gatcggcgat ggtgtcaatg ctcgacaact
    15181 catgtgtgac gatgacgaac gtgatcgcga gattgcgggc gaggtcgaga atcagtcggt
    15241 cgagttcggc cgaggtaatc gggtcgagtc ccgcagacgg ctcatccaga aagagaatgt
    15301 gcgggtcaag cgccatggcg cgcgcgatag ccgcacgttt ctgcatacca ccactgagtt
    15361 cggacggcat ataatccagg aacggttcga gccccaccaa tttgagtttc atgcgagcga
    15421 tcagatccat ggcatccgac ggaagggcgg tgtattcttc caggggcagc cggacatttt
    15481 ccaacagggt catggagccg aacagggcac ccatctgata cgtgactcca atgctcttga
    15541 gaatactggt gcgctcctct ccctcggccg tcacgatgtc cttcccgccg atgacaatgt
    15601 gcccggccgc gggctggtac agaccgatca tgtgtttcag caaggtgctc tttccgctgc
    15661 cggatccccc gaggatggca aacacctcgc cacgatagat ctcaaacgag atgtcgtcga
    15721 tcaccactga cccatcgtag gcggcggtga agtgttgcac ttgaa
//
LOCUS       DAOQDS010000082        15657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1582_length_15657_cov_20.203307, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000082 DAOQDS010000000
VERSION     DAOQDS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15657
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1582_length_15657_cov_20.203307"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /gene="dnaK"
                     /locus_tag="PK589_15950"
     CDS             complement(<1..1528)
                     /gene="dnaK"
                     /locus_tag="PK589_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNJ21154.1"
                     /translation="MGKVIGIDLGTTNSCVAIMSGGDPVVIANAEGSRTTPSVVGITD
                     KNERLVGQIAKRQAITNPENTIFSVKRLMGRKFKSKEVQEAMKRLPYKVVEADNGDAH
                     VELRGKRYSPPEVSAMILQKMRQTAEDYLGEKVTEAVVTVPAYFDDSQRQATKDAGQI
                     AGLNVLRIINEPTAASLAYGLDKKKDERIAVYDLGGGTFDVSVLEIGDGVFEVKSTNG
                     DTYLGGDDFDERVMDWLVEEFKKDQGIDLRKDRMALQRLKEAAERAKIELSSSQESEI
                     NLPFITADASGPKHMVTKLTRAKLEQLVDDLIQKTIEPCRKALADAGVSAKDIQEVVL
                     VGGMTRMPKVIQVVKDFFGKEPHRGVNPDEVVAIGAGVQGGVLKGEVKDVLLLDVTPL
                     SLGIETLGGVFTKLIERNTTIPTKKSQVFSTAADNQTAVTIRVFQGEREMANDNKLLG
                     QFDLVGIPPAPRGMPQVEVSFDIDANGIVHVAAKDLATQKEQSIKITASSGLSKEEVD
                     K"
     gene            complement(1611..2174)
                     /gene="grpE"
                     /locus_tag="PK589_15955"
     CDS             complement(1611..2174)
                     /gene="grpE"
                     /locus_tag="PK589_15955"
                     /inference="COORDINATES: protein motif:HMM:NF010748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNJ21155.1"
                     /translation="MQSMSEDEKNVHSIDNLDSSDEPSSATIDGASELQQVLDAKAEE
                     CKAAHEKYLRLAAEFDNYKRLAQRDQREQIKFGNEQILKELLPVVDNLERAIKSAKGA
                     GSVDALTEGVELTLKQLVGALSKFGVKAVESVGQVFDPATQQAVAQVPSDTIPENHVV
                     EEYQKGYLLQDRILRAAMVTVSTGAAN"
     gene            complement(2303..3073)
                     /locus_tag="PK589_15960"
     CDS             complement(2303..3073)
                     /locus_tag="PK589_15960"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNJ21156.1"
                     /translation="MLLAIDIGNTNVVCGLFEGSTLRGHWRMATESRRTDNEYGILLL
                     NLLQNAGFTPDQITGCVLSSVVPALTATFDSVVQEYFHRTAVIVGPETDSGLTLRYAN
                     PKEIGSDRIVNAAAAYARYHSDVIIVDFGTATTFCAVTKSAEYLGGVIAPGLGISADA
                     LFARTAKLPKVEIIRPKTVIGKDTIGGIQSGLLFGYVGLVDGIVRRMERELGRTSRVV
                     ATGGLATVIAQETETIQEVLPFLTLEGLELLYRRNRLA"
     gene            complement(3109..3981)
                     /locus_tag="PK589_15965"
     CDS             complement(3109..3981)
                     /locus_tag="PK589_15965"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNJ21157.1"
                     /translation="MGGPISDSPISPPFDIDRLQASLRTHAFGRPLRYSASTASTNAD
                     ALASLQQSATKPAPHGLVILADCQTAGRGRRGRTWHSPPQGNLYLSVIVVPRPVATGT
                     TPWLTWIPLLSALAGADCLSQQTGLPVSVKWPNDLLIHDKKVGGILCEQTTTPDRTMA
                     IIIGIGLNINAPLNTFPEDLQQGATTLAAEAGHEFDRVAILADLLFRLEQRLNRLFLE
                     GPAGMIDEFTQRCSTVGQTVRVTLEEKGMVQGIAESIGPDGCLRLRVTSNESPSLPST
                     LLEVRSAEVVHLRG"
     gene            complement(3965..4846)
                     /gene="nadC"
                     /locus_tag="PK589_15970"
     CDS             complement(3965..4846)
                     /gene="nadC"
                     /locus_tag="PK589_15970"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNJ21158.1"
                     /translation="MSTPSPQTILNAVQQALAEDLYHGDVTTSALFPRPLRATATIVA
                     HQPMTVAGVAVAREVFLAVDPSARITKAVKDGSAVKSGTTVLTVQGDVRSLLMAERVA
                     VNFLQQLSGIATLTAQFCAAVRGYPTRILDTRKTTPGLRALEKWAVQLGGGKNHRFSL
                     GDGVLIKDNHLAVLRSTGIDVAGACRLARAGAPHGLRIEVEAKTMQEVKEALAGKADI
                     ILLDNMSPAQVRQAVTLIKQRALVEVSGGMTLQTVAAMAQVGADFISVGALTHSAPAA
                     NLSMDLSAQRGRRGRSH"
     gene            complement(4833..7595)
                     /locus_tag="PK589_15975"
     CDS             complement(4833..7595)
                     /locus_tag="PK589_15975"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNJ21159.1"
                     /translation="MSTPQLDKTYNPHDVENRWYQRWIDAGLFHADPAQTGQPYSMVI
                     PPPNVTGSLHVGHALNNTLQDILIRWRRMQGRNVLWMPGTDHAGIATQNVVERQLQAE
                     GTSREALGREAFLSRVWQWKEKSGGTIIGQLKKLGASCDWQRERFTMDEGLSEAVREV
                     FVRLHQDGLLYRGERLINWCPRCLTALSDIEVEHEDATGKLYTIRYHLVDDPTQFLLV
                     ATTRPETLFGDTAVAVHPEDDRFRHLIGKHVRLPLTGRAIPIVGDSILVDREFGTGAV
                     KITPAHDFNDFEAGERHGLKRIKILDLHAHLRLGGSLADPAVAEAVENLPVAKARPKI
                     EQFLTDQGFLEKSEPHKMALGKCYRCKTVVEPFLSDQWFVKIKPLAEPAIQVVEDGRV
                     RIIPEAWKNNYLGWMRDIKDWCVSRQIWWGHQIPAWYCETCSGTPFLRRSSDGAPLIP
                     SDAAAIVAKTKPDSCPLGHRDALVQDPDVLDTWFSSALWPFSTLGWPKQTPELKAFYP
                     TSTLVTGLDILFFWVARMIMMGLKFMGDVPFRDVYIHALVRDAEGQKMSKSKGNVIDP
                     LHVMEQYGTDALRFTLAAMASPGRDVKLAEERIEGYRNFTNKIWNAARFLLMHLDGER
                     TDVAPAQRSFPDRWILSRLNTAISTVTQELEQYRFDRASSAVYQFIWHEYCDKYIEMV
                     KPALKDANSEQAKSTRQTLAETYETMMRLLHPFMPFITEEIWQTLPHEGTSIVRKSFP
                     TTRKDWESEEVEQEFALLEECRALMNQERAILGYPAGKRLHFKVHGKTEAIASILQKH
                     KDLIEYMENVDNLWIAKTFDVSNPGGLLILTSGSIQVRTSMEDADLGKAEENVQKQIK
                     LLQKEVDRTQQKLGNPDFVAKAPPDVLTEHRERLQRESHMIQLFQQALDQIKLYTLER
                     TRIEHP"
     assembly_gap    8090..8189
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8190..9200)
                     /locus_tag="PK589_15980"
     CDS             complement(<8190..9200)
                     /locus_tag="PK589_15980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ21160.1"
                     /translation="MGSTIFVIDNSPAVQRMVEQLSAPDGHRVLAFQDGPSALEAARS
                     QCPALIIADYHLEQMTFSGFCKEIGRQDNLAETLIVSIVDSADRLDESKLRALGVRAF
                     LKKPFQREQLLETIQSILSKPEGSRSQKPAKSRTWPPATTGADDDDETASDASAEHTS
                     RQAEKKEQAPMSSTAPATRPTGEDLMKGLVEHLLHSTTVKADRAITELLPAAVAKEVA
                     GQLGAALSKAVQAEVSKQVAEALAPERIQNTLREMVQMELKRQSESQRAGMESSIRQA
                     VTELAPGLMAQSSEKLLPGLTDAGVKQHLPEALQSQRDLITDGVKKEVEQVAATYARQ
                     AAE"
     gene            9413..10138
                     /locus_tag="PK589_15985"
     CDS             9413..10138
                     /locus_tag="PK589_15985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNJ21161.1"
                     /translation="MSQSRIQVVFFDAADTLFHIQGSVAEIYLQHAERHGFRKTPDSL
                     AAIKAAFARSFRDAPPPVFAATEPGGIKQSERLWWFDIVHNVFYRVGMFEKFDEFFED
                     VFARFAQAESWKLFPETLDVLKTLKDHGYELGIISNFDSRLFSVLRGLGIADFFDTVT
                     ISSLAHAAKPSARIFEQALEKHAVDPEDALHVGDSARDDVKGAIAVGLTGVLLARGLP
                     PEGSNGATIATLNELLPLLSRLQ"
     gene            complement(10124..10855)
                     /locus_tag="PK589_15990"
     CDS             complement(10124..10855)
                     /locus_tag="PK589_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNJ21162.1"
                     /translation="METGSTLGLVRHKLSTARSVTILTGAGISADSGVPTFRGADGLW
                     RHYRAEDLATPEAFARDPRLVWEWYNWRRELIATKRPNPAHEAVASMEQRFKQFWLIT
                     QNVDGLHGDAGSRQLSEIHGNLWKVRCTRCRDIVENRGVPIAMLPLCRSCGGLLRPHI
                     VWFGESLAEEDLDRSAAALQSSDVCLIVGTSGMVYPAAGFGAIAKQAGAFIVEINLDA
                     TAHSNLADATLQGRARDVVPLLLEA"
     gene            10940..12307
                     /locus_tag="PK589_15995"
     CDS             10940..12307
                     /locus_tag="PK589_15995"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNJ21163.1"
                     /translation="MKQIMVVGAGSVGGFFGAHLAKNNPNVSFLLRPRTLEAVKRNGL
                     TIKSAKGNFTVHPPAASDPRELATPDLIILAVKAYDLDEVMTQLEPVLTDRTVILTLQ
                     NGIDTEDRIISRLHRDCVVGGVAFIYSKIVEPGVIEHYKRGGVAIGELMGHKSERVSQ
                     IADIFKQAGISCQLSEDIRKSKWEKMCWNCVFNPLTVMIDDKVAKALDHPEMAGVIRQ
                     IVGEVAAVSAAVKVPLAPDMAEKVVKWTQELRDIHTSMYDDWKAKRPTEIDYLNGYIV
                     RVGRELGIPTPVNEALTAMVKTITEKELSGPSIVRIDGAVVQPVSLTRTALGQLPREQ
                     RVDDISELMPSMRGRAIRVKGLLEVPALAVDADHVTFHSVDGKYAATLTLQQAQDFGL
                     LLYELDGQPLPDGKGGPYRLVTPGLGDLCANVKAVGRIEVRAGSGKDTRPAVRPPECA
                     VDGQS"
     gene            complement(12313..13650)
                     /locus_tag="PK589_16000"
     CDS             complement(12313..13650)
                     /locus_tag="PK589_16000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNJ21164.1"
                     /translation="MAQGIAQIRRSVLTLALPVTVSSLLQRAEGIVAVFLVGGLGAVP
                     IAAVGLGQLLAFIATTLVSGLSVGSNVVMAQLWGARRYDDAGHAARHFLGLSILIALG
                     LLSFGLLVNRLAMEMLGAQADVIRLALPYSNLIFLLIPFTILLQVLSSILQGTGDTKT
                     PMYAMIVVNLLHIAIAIPLIYGHWGLPALGINGAAIAVGIAEATGCLYLFTRCRPMLR
                     GSHRFRFDLIRTIWHVGAPVSGERVVQQAGILLYTKIVLMYGTVAYAAHQVGLSIESL
                     SFLPGYGFAIAAATMVGQSIGAGKYTRAKLETWEANRLAAYMMSAMGVVFFLFPYALL
                     RAFTSDEAVVELGTLFLKIVALLQIPLALTMVVAGSLRGAGDTRFVMIATMIGMWGVR
                     IPLAIVAGYWLSMGVLYVWLAMIADWTVRMALMLWRHRSERWRSIQVLRSSPT"
     gene            13753..15258
                     /locus_tag="PK589_16005"
     CDS             13753..15258
                     /locus_tag="PK589_16005"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HNJ21165.1"
                     /translation="MLILPEPAARSSYRLSKSRYLSGLQCHKRLYLEVHAPGLATKPD
                     AATQAILDMGTDLGELARQRFPGGRLVTAGYRQSQEALAQTAELLQDPTVPAIFEGAF
                     QFNQVLVRVDVLQRVGVNELGQPTWRLVEVKSSTKVKATHVDDLTIQSYVLEGLGLVL
                     AEICLMHVNTQYVFDGRYLDLDQLFGLRSLTETVRPRLPEVPARLAAMQAMLEGMSAP
                     AVMPDEHCQSPYECPFWTHCIEHKPARWIYHLPGSSKTVVQLRELGVETIDEIPDHVA
                     LTPVQRRVKDNREWIGEGLRSALETIVYPVHHLDFETFMPAVPKFVDTRPYQVIPTQW
                     SNHIEQYDGTLTHAEFLCRDERDPREELAITLLESLGQTGSICVYSSYERSVLERLAE
                     DFPSLRKDLKRVVARLWDLHVIIRDHYYHPAFEGSYSIKAVLPAVMPSLSYADLVIRD
                     GGVAACEYSRMIFTVTDWIEKAQIQEALLRYCERDTLAMVELRRELKRRAG"
     gene            complement(15255..15389)
                     /locus_tag="PK589_16010"
     CDS             complement(15255..15389)
                     /locus_tag="PK589_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21166.1"
                     /translation="MFEQTDAALVMTLLALGGIALMADEKLGTTCLQMVRWIRTRGRH
                     "
     gene            complement(15446..>15657)
                     /locus_tag="PK589_16015"
     CDS             complement(15446..>15657)
                     /locus_tag="PK589_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21167.1"
                     /translation="KTVVLKGTVGSNPTLSAIPDPTPRFLPVGVEPAPPWNLPLCFAE
                     LSGISLRIAARRAAAPTEDRAVPFL"
BASE COUNT         3056 a         4366 c         4785 g         3350 t
ORIGIN      
        1 gcttgtccac ttcctctttg ctgagtccgc tggacgccgt gatcttgatg gactgctctt
       61 tctgcgtcgc cagatctttg gcggcgacgt ggacgatgcc attggcatcg atatcgaagg
      121 agacttccac ctgcggcatg ccgcgtgggg ccgggggaat gccgacgagg tcaaactgcc
      181 ccagcagctt gttgtcattg gccatctcac gttcgccctg gaagacccgg atcgttacgg
      241 cggtttgatt gtcggccgcc gttgagaaca cctggctctt ctttgtcgga atcgtcgtat
      301 tccgctcgat cagtttggta aagacgccgc cgagggtttc gatgccaagg gacaacggag
      361 tcacgtcgag cagcagcaca tccttgactt cgcccttcag tacgccgcct tgaacaccgg
      421 cgccgatggc cacgacttca tccgggttca caccgcggtg gggttctttc ccgaaaaagt
      481 ccttcacgac ctgaatgact ttcggcatac gggtcatgcc gccgaccagg acgacttcct
      541 ggatatcctt cgcgctcact cccgcatcag ccaatgcctt gcggcagggt tcgatggtct
      601 tctgaatgag atcgtcaacc agctgctcca atttcgcgcg ggtcagcttg gtcaccatgt
      661 gcttcgggcc gctggcgtct gccgtgatga acggcagatt gatttcgctt tcctgagagg
      721 aggacagttc gatcttcgcc cgctcggccg cttccttcaa gcgttggagc gccatgcggt
      781 ccttgcggag gtcgatgccc tgatccttct taaattcttc gacgagccag tccatcacgc
      841 gttcatcgaa atcgtcgcca ccgaggtagg tgtcgccgtt cgtggacttc acttcaaaca
      901 cgccatcgcc gatctcgagc acggacacgt cgaaggtgcc gccacccaag tcgtacacgg
      961 cgatgcgttc atcttttttc ttatcgagac cgtaggcgag cgaggccgcc gtcggttcgt
     1021 tgatgatgcg gaggacgttc aatccggcga tttgcccggc gtccttggtc gcctggcgtt
     1081 ggctgtcatc aaaataggcg ggaaccgtca ccacggcttc cgtgaccttt tcgcccagat
     1141 agtcttcagc cgtctgccgc atcttctgca ggatcatggc cgagacttcc ggcgggctgt
     1201 accgtttgcc ccgcaactcg acgtgggcgt cgccattgtc tgcctccacg accttgtagg
     1261 gcaggcgctt catggcttcc tggacttcct tgctcttgaa tttgcgtccc atcaggcgct
     1321 tcacggagaa gatggtgttc tcaggattcg taatggcttg ccgtttggcg atttgtccca
     1381 ccaagcgctc attcttatcc gtgatgccca ccactgacgg agtggtccga cttccttccg
     1441 cattcgcgat gacaaccggg tctccaccgc tcatgatggc gacgcaggag ttcgtcgtgc
     1501 cgaggtcgat tccaataact ttgcccatgg gttggctcct tcgcaaaaaa tatcaatacg
     1561 tgaacgatcg tgtcagctgt gctcgcgaaa caagttgcgt acgtaccgtc ttaattcgcc
     1621 gccccggtcg atacggtgac catggctgcc cggaggatgc gatcttggag gagatatcct
     1681 ttttgatact cttccacaac gtgattttca ggaatcgtgt ccgacggcac ttgcgcgact
     1741 gcctgttggg tagccggatc gaacacctgg cccacactct ccaccgcttt gacgccgaac
     1801 tttgataggg cacccacgag ttgcttgagt gtcaactcga cgccttccgt gagggcatcc
     1861 acagagcccg cccctttggc ggatttgatg gctcgttcta agttatcgac gacggggaga
     1921 agctctttca gaatttgttc gttgccgaat ttgatttgct cgcgttggtc gcgttgggcc
     1981 agccgcttgt aattgtcgaa ttcggctgcc agtcgcagat acttttcgtg agcggctttg
     2041 cactcctcgg ctttcgcatc cagcacctgc tgcaattcgc ttgcaccgtc aatcgtcgca
     2101 gaggagggtt cgtctgaact atctaagttg tcgatactgt gaacgttctt ctcgtcttca
     2161 gacattgatt gcaccttttc tggtgagtga gatagccacc ggaggatgga agtcaacggg
     2221 gaatgaaaag aaatagtcgt tttttcgagt ctctcagcgt tcgcgggatg gaatgttgtg
     2281 acagcacccg tcggaaaggc gcctaagcga ggcgattccg gcggtacagc agttccaagc
     2341 cttcgagggt gaggaaggga aggacttcct ggatcgtttc agtctcctgt gcaatgacgg
     2401 tcgccagacc gccggtggcg acgactcgag aggtgcggcc aagttcccgt tccatgcgtc
     2461 ggacaatgcc gtcgaccagt cccacatagc cgaacaggag gccgctttga atgcccccga
     2521 tcgtatcctt gccgatgacg gttttgggcc tgatgatttc gactttcggc agtttcgcgg
     2581 tgcgggcaaa cagggcgtcg gctgaaatgc cgagacccgg tgcgataacg ccgccgaggt
     2641 actccgccga tttggttacg gcgcagaacg tcgtagcggt accgaagtcg acgataatga
     2701 catccgagtg atagcgcgca taagccgcgg cggcgttgac gatacggtcg ctgccgattt
     2761 ctttggggtt cgcgtagcgc agcgtcaagc cggagtcagt ctcaggccct acgatgacgg
     2821 cggtgcgatg aaaatactcc tgcaccacgg aatcgaaggt ggcggtgagc gcaggaacga
     2881 cactggagag gacacagccg gtgatctggt ccggggtaaa gcccgcgttt tggagcagat
     2941 tcagcagcag gatgccatac tcattgtcgg tacgtctgga ttcggtggcc atgcgccaat
     3001 gcccgcgcag ggtcgagcct tcgaacaggc cgcagactac atttgtgttg ccgatgtcga
     3061 ttgccaacag catcgtgcct tcactctacc ccaagccgta gcaggatttc acccccgcag
     3121 atggacgacc tcggcgctcc ggacttctaa caatgtcgaa ggaagggacg gggactcgtt
     3181 cgatgtcacc cgcaggcgga ggcagccatc agggccgatg gactcggcga ttccttgaac
     3241 catccccttt tcttcgaggg tgacccggac ggtttggccc acggtcgagc agcgttgggt
     3301 aaattcatcg atcatccccg cgggaccctc gagaaacagc cgattcagtc gttgttccag
     3361 tcgaaacagc aggtcagcca gaatggccac gcgatcaaat tcatggccgg cttctgcggc
     3421 cagggtggtg gcaccttgtt gaagatcttc aggaaaggtg ttgagcggcg cattaatgtt
     3481 cagaccgatg ccgataatga tggccatcgt tcgatctggc gtagtggttt gctcgcacag
     3541 gatgccgccg accttcttgt cgtggatgag cagatcattc ggccatttca cggaaaccgg
     3601 aagccctgtc tgttgagaga ggcagtcggc tcccgcgagg gcggataaca gcggaatcca
     3661 ggtcagccag ggtgttgtgc ctgtggcgac aggtcgaggt accacgatga ccgagagata
     3721 cagattgccc tgcggcggtg aatgccaggt tcgtccccga cgtccacgtc ccgccgtttg
     3781 gcaatcagca agaatgacca atccatgagg ggcgggcttt gtcgccgatt gttgcaggga
     3841 tgcgagagca tcggcattgg tggacgcggt cgaggctgaa tagcgcaacg gtctgccgaa
     3901 ggcgtgggtg cgcagggagg cttggaggcg atcaatgtcg aaaggagggg aaatcggcga
     3961 atcgctaatg ggaccgccca cggcgtcctc gctgtgctga gagatccatg ctgaggttgg
     4021 ctgcgggcgc ggaatgggtc aaggcgccga ccgaaatgaa atctgcaccg acctgggcca
     4081 tggcggcgac cgtttgcagg gtcattccac cagacacttc gaccaaggcg cgttgtttga
     4141 tgagtgtcac ggcctggcgc acctgggcag gcgacatatt gtcgagcaga atgatatcgg
     4201 ccttgcccgc taaggcttct ttcacttcct gcatggtttt ggcttccacc tcgatacgaa
     4261 ggccatgagg cgcaccggct cgcgcgaggc gacaggctcc tgccacatca atgccggtcg
     4321 agcgcaaaac ggcgaggtgg ttgtccttga tgagcacacc gtcgcccaga gaaaatcgat
     4381 gattcttgcc gcctcccagc tgcaccgccc acttttcgag ggcccgcaat cctggcgtgg
     4441 tcttgcgagt atcgaggatt ctggttggat agccacgcac tgcggcacaa aactgcgcgg
     4501 tgagtgtggc gatgcctgac agctgctgga ggaagttgac cgcaacgcgt tcggccatca
     4561 gtagcgagcg cacatcgcct tgaaccgtca gtaccgtggt ccccgatttt acggcgctgc
     4621 catccttgac ggctttggtg atgcgggccg aggggtctac agcgaggaac acttcccggg
     4681 cgacggccac acctgccacg gtcattggtt gatgggctac gatggtcgca gtggcgcgaa
     4741 gtggcctggg aaacaacgca ctggtggtga cgtcgccatg gtagagatct tcggcgagcg
     4801 cctgttgcac ggcgttgagg atcgtctggg ggctaggggt gctcaatacg tgttcgttcc
     4861 aaggtataga gcttgatctg gtccagcgcc tgttgaaaaa gttgaatcat gtgagattcg
     4921 cgctgaagcc gctcgcggtg ttcggtcagt acgtcaggtg gcgccttggc cacgaaatcg
     4981 ggattgccca gtttttgctg cgtgcggtcg acttcttttt gaagcaactt gatttgtttc
     5041 tgaacatttt cttcggcttt tccaaggtcc gcatcttcca tcgaggtccg cacttggata
     5101 gagccgctcg tcaggatcag aagacctcct ggattgctga cgtcgaacgt cttggcaatc
     5161 cagagattgt ctacgttctc catgtattcg atcaggtctt tatgtttctg aaggatggat
     5221 gcaatcgctt ctgtcttgcc gtgcactttg aagtggagac gtttccctgc agggtatccg
     5281 agaatcgccc gctcctggtt catcagggcg cgacattctt ccagcagcgc gaactcctgt
     5341 tcgacttctt ctgactccca atcctttctc gtggtcggga aggacttgcg gacgatgctg
     5401 gttccttcat gcggcagggt ctgccagatt tcttcggtaa tgaacggcat gaacggatgc
     5461 agcaatcgca tcatggtttc atatgtctcg gccaatgttt gccgcgtact cttcgcctgt
     5521 tcggagttcg catccttgag cgcaggcttc accatctcga tgtacttgtc gcagtactcg
     5581 tgccagatga attgatagac cgcactggag gcgcgatcga accgatactg ctccaattcc
     5641 tgggtgaccg tgctgatggc ggtattcagg cgactcagaa tccagcggtc tggaaacgat
     5701 cgttgtgccg gcgcgacgtc ggtgcgttcg ccgtccaggt gcatgagcag aaaccgtgcg
     5761 gcgttccaga ttttgttcgt aaagttgcga tagccctcga tacgttcttc ggccagtttc
     5821 acatcgcgcc cgggggaggc catggctgcc agggtaaagc gcagcgcatc cgtgccatat
     5881 tgttccatca catgcagcgg atcgatgacg ttgcccttcg acttgctcat cttctgccct
     5941 tcggcatcgc ggacgagcgc gtggatgtag acgtcgcgga acggcacatc gcccatgaat
     6001 ttcaacccca tcatgatcat gcgggcgacc caaaagaaca gaatgtcgag cccggtcacg
     6061 agggtagagg tcggatagaa ggctttcaat tcgggcgtct gcttgggcca tcccagcgtc
     6121 gaaaacggcc agagggcaga ggagaaccag gtgtccagga catcgggatc ttgcacgagc
     6181 gcgtcgcggt ggcccagcgg acaggagtcc ggtttggtct tcgccacgat cgccgctgca
     6241 tccgacggta tgagcggggc gccatcggaa cttcgccgta aaaacggagt gccggaacag
     6301 gtttcgcaat accaggcggg gatctgatgg ccccaccaga tctgccgcga gacgcaccag
     6361 tccttgatgt cccgcatcca gcccagatag ttgtttttcc aggcttcagg gataattcgg
     6421 acgcgaccgt cttcgacgac ctgaatcgcc ggttcggcca gcggcttgat cttgacgaac
     6481 cattggtctg acaagaacgg ttcaaccacc gtcttgcagc gataacattt gccgagtgcc
     6541 atcttgtggg gctcggattt ttccagaaat ccctgatcgg tgaggaattg ttcgatcttc
     6601 gggcgagcct tcgcgacagg aagattctcg actgcctctg cgacggcggg atcggccaaa
     6661 gagccgccca atcgaaggtg ggcgtggaga tcgaggatct tgatccgttt cagtccgtgt
     6721 cgctctcccg cctcaaaatc gttgaagtcg tgggcaggcg tgatcttgac ggcgccggtg
     6781 ccgaattcgc gatcgaccag gatcgaatcg cccacgatgg ggattgcgcg gcccgtcaat
     6841 ggtaaacgga cgtgcttgcc gatcaagtgg cggaaacggt cgtcttccgg gtgcacagcc
     6901 accgccgtgt cgccgaacag ggtttcaggg cgcgtggtcg ccacaaggag aaactgtgtc
     6961 ggatcatcta ccaggtgata gcggatcgtg tacagttttc cggtcgcgtc ttcgtgttcg
     7021 acttcgatgt cggacagagc ggtcagacat cgcgggcacc agttaattaa ccgctcaccg
     7081 cggtatagga ggccatcctg atgcaagcga acgaagactt cgcggaccgc ttcggagagc
     7141 ccttcgtcca tggtgaagcg ctcacgctgc caatcgcagg aggctccgag tttcttgagc
     7201 tggccgatga tcgtcccgcc ggatttttcc ttccactgcc acacgcgcga gagaaatgcc
     7261 tctcgaccca gtgcttcccg tgaggtgcct tcggcttgca gttgccgctc cacgacattc
     7321 tgcgtcgcaa ttccggcatg atccgttccg ggcatccaca gcacgttgcg accttgcatg
     7381 cggcgccagc ggatcaggat gtcttgcagg gtgttgttca gggcatgacc cacatgcagc
     7441 gatcccgtga cattcggcgg agggatcacc atggagtagg gttgcccggt ctgggccgga
     7501 tcggcatgaa acagtccggc atcgatccag cgctggtacc agcggttttc gacgtcgtgt
     7561 ggattgtagg ttttatcgag ttgtggggtg gacataagag gcgcacactg cggcgcgggc
     7621 gcatcttatc atgtgctgct tgtgcgctca aggtaacgac ggtgcatctg tggcggtgca
     7681 aaaggttgta gcggagtgaa gccctgtgat atacatccgg cggttcagtc gatggcgagt
     7741 gcgctcgccg gccagcatgc cgattttacc gttgaaggag agactatgcc gaggggacgt
     7801 gagaaggatc ggaaagttcg gaagaaacac cgcaagaatg tggcgcgcgt gaaggcgctg
     7861 gtcaagaccc gtcgcgcggc ggctaaaaag gtgaagcgag gctaacgccg tgaggtgtta
     7921 gtcgggtgag ctcaatcgtg tgatctcggc ccggatctga gtttccgcca cgtcaggaac
     7981 gacccgttgc accgcctgca ggatcgggtc tttcgccaat tcccgcacaa tgtcctcggc
     8041 cgcttggcgt gcataggtcg cggccacctg ctcgacttct tttttcactn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tcggccgctt ggcgtgcata ggtcgcggcc
     8221 acctgctcga cttctttttt cactccatcc gtgatcaggt cgcgttggct ctgcaaggct
     8281 tccggcagat gttgcttcac gccagcgtcc gtgagccctg gaagtaattt ttccgaagac
     8341 tgcgccatga ggccaggagc caattccgtc actgcttgtc ggatgctgga ctccatgccg
     8401 gcacgttgcg actcgctctg acgtttcaat tccatctgca ccatttcgcg gagggtgttc
     8461 tggatccgtt ccggggcgag cgcttccgca acctgtttgc tcacttcggc ctgcacggcc
     8521 ttgctgagcg cggcccctaa ttggcccgca acctccttgg caacggcggc gggcaacaac
     8581 tcggtgatgg cccggtcggc tttgacggtc gttgagtgga ggagatgttc gaccagcccc
     8641 ttcatcaagt cctcgcccgt gggtctggtt gcgggtgctg ttgacgacat gggggcctgc
     8701 tccttcttct ccgcctgtcg ggaggtgtgt tcagcagagg catccgacgc ggtctcgtca
     8761 tcgtcgtccg ctcccgtggt ggccggaggc caggtgcgag acttggctgg tttctggctg
     8821 cgggatcctt ccggtttgct gagaatgctc tgaatcgtct caaggagttg ttcccgttga
     8881 aaaggctttt tgagaaacgc ccgcacgcca agcgcgcgca gcttgctttc atcgaggcga
     8941 tcggcactat cgacgattga gacgatgagc gtttcggcca gattgtcctg tcgtccgatt
     9001 tctttgcaga atccggaaaa agtcatctgc tcgagatggt aatcggcaat gatcagcgcg
     9061 gggcattgtg atcgggcggc ttcaagggcg gaggggccgt cttgaaacgc caatacccga
     9121 tgcccatcgg gggcggagag ttgctccacc atgcgctgca cggccggact gttgtcgatg
     9181 acgaagatcg tggaacccat tggaaacacc tcgctctacc cactccagcg aagctaacaa
     9241 agggtctgaa aactgtcaaa gaaaacgccg aatccaactg ttctgacgag gcaggggtgg
     9301 gcctcgggcg ctcagcgctg gcggaggttt tacgcatcct gatacaatcc gcccaccgtg
     9361 ccggttggcg ggtgctccgt gagtgccgaa gagagaggag tgaatccata cgatgagtca
     9421 gagccgcatc caagtcgtgt ttttcgacgc tgccgatacc ctctttcaca ttcaaggatc
     9481 cgtggcggaa atttatctcc agcatgcgga gcgccacggc tttcgcaaaa cgcctgactc
     9541 gttagcggca atcaaggcgg cgtttgcccg ctcgtttcgc gatgcgccgc cgccggtatt
     9601 tgccgcgacg gaaccgggcg ggattaaaca atcggagcgc ctgtggtggt tcgatatcgt
     9661 ccacaacgtc ttttatcgcg tcggcatgtt cgagaagttt gatgagtttt ttgaggatgt
     9721 gtttgcacgt tttgcgcaag cggagtcctg gaagctgttt ccggagacgc tggatgtgct
     9781 caagaccctc aaggatcacg gatacgagtt ggggatcatt tcgaattttg actcgcggct
     9841 gttttccgtg ctgcgcgggc tcgggattgc cgacttcttc gatacggtca cgatatccag
     9901 cctcgctcac gccgcgaagc cgtccgcgcg aattttcgag caggcattgg agaaacatgc
     9961 cgtcgacccg gaggacgcgc tgcatgtcgg cgatagcgcg cgtgatgatg tgaagggcgc
    10021 aatcgcggtc ggactgacgg gggtattgct ggcgagaggg ctgccgccag agggatcgaa
    10081 cggtgcgacg atcgcaacgt tgaacgaact cctgccgctc ttgtcacgcc tccagtaaca
    10141 atggcacgac gtctcgcgca cgcccctgca acgtggcgtc ggccagattc gagtgcgccg
    10201 tcgcgtcgag gttgatctcc acgataaacg cgcccgcctg cttggcgatg gcgccgaatc
    10261 ccgccgccgg atagaccatc ccggacgtac cgacgatcaa gcagacatcg ctgctctgta
    10321 gggcggcggc actcctgtcc agatcctctt ccgccaggga ttccccgaac cagacgatgt
    10381 gcgggcgaag cagtccgccg caagaccggc agaggggcag catggcgatg ggtacgcccc
    10441 gattttcgac aatgtcccgg catcgggtgc agcggacctt ccagaggtta ccgtgaattt
    10501 cggataactg ccgcgagccg gcgtcaccgt gcagcccgtc tacattctgc gtgatgagcc
    10561 agaactgctt gaagcgctgc tccatcgacg ccaccgcttc gtgtgccgga ttcggtcgtt
    10621 tggtggcgat gagttcccgg cgccagttgt accattccca caccaggcgc gggtcacgcg
    10681 cgaaggcttc cggtgtggcg agatcctctg cgcgatagtg tcgccagagc ccgtcggcgc
    10741 cgcgaaatgt cgggacgccg ctgtcggcgg agatcccggc tccggtcagg attgtgacag
    10801 accgcgccgt ggagagtttg tggcggacaa ggccgagagt ggatccggtt tccataaagg
    10861 tcagtctccc ctcactgttt caggtgcgcg gctgcatgct atagtacgcg tcttttgaac
    10921 gcaacgagtg aggcagctga tgaagcagat catggtggtc ggtgctggtt ccgtcggtgg
    10981 attcttcggg gcgcatctcg ccaagaacaa tcccaacgtg tcatttcttc ttcggcctcg
    11041 cacgttggag gcggtgaagc ggaacggcct cacgatcaag agcgccaagg gaaatttcac
    11101 cgtccatccg ccggccgcct cggatccccg ggagcttgcg acacctgatc tcatcatcct
    11161 ggccgtgaag gcctacgacc tcgacgaggt catgacgcag ttggaaccgg tgctgacgga
    11221 tcgcacggtg attctcaccc tgcagaacgg catcgataca gaagatcgca tcatatcccg
    11281 cctgcatcgg gactgcgtgg tcggcggggt ggccttcatc tattcgaaaa ttgtcgaacc
    11341 aggcgtcatt gagcattaca agcgcggcgg ggtggcgatc ggggaactga tgggccacaa
    11401 gagcgagcgt gtgtcccaga tcgccgacat tttcaagcag gcagggattt cctgccaact
    11461 gagcgaagat atccggaaaa gcaaatggga gaagatgtgc tggaattgtg tcttcaatcc
    11521 tctcacggtc atgatcgacg acaaggtggc gaaggcactc gatcatccgg aaatggccgg
    11581 cgtcatccgg cagattgtcg gcgaagtggc cgcggtgtcc gcggcggtga aggttccgct
    11641 ggcgcctgac atggccgaga aggtggtgaa gtggacgcag gagctgcgtg acatccatac
    11701 gtcgatgtac gacgattgga aagccaaacg cccgacggag atcgactatc tgaatggcta
    11761 catcgtgcgg gtgggacgcg agttgggtat tcccacaccg gtgaatgaag cgctgacggc
    11821 gatggtcaag acgatcactg aaaaggaact gtcagggccg agcattgtgc gcatcgacgg
    11881 cgcggtcgtt cagccggtct cattgacgag aacagcgctg gggcaattgc cgcgggagca
    11941 acgggtggac gatatcagtg agttgatgcc gtccatgcgg ggtcgcgcca tccgcgtgaa
    12001 gggcctgctg gaggttccgg cgcttgcggt ggacgcggac catgtgacgt ttcactccgt
    12061 cgatggcaag tatgccgcga ccctcacgct acagcaggcg caggacttcg ggctgttgtt
    12121 gtacgagctg gatgggcagc ctcttccgga cggcaaaggt ggcccctatc gtttggtgac
    12181 gcctggtctg ggcgatctct gcgccaatgt gaaggccgtg ggccgcatcg aagtacgagc
    12241 cggatcgggg aaggatacgc ggccggccgt gcgtccgccg gaatgtgcag tggacggcca
    12301 gagctgaata cctcaagtgg gagaggaccg gagcacttga atgcttctcc accgctccga
    12361 acggtgtcgc cagagcatca atgccatgcg taccgtccag tcggcgatca tcgccagcca
    12421 tacgtacaac actcccatgg aaagccagta gcccgccacg atggccaggg gaatccgtac
    12481 gccccacatg ccgatcatcg tcgcgatcat gacgaaccgg gtatcacccg ctccccgcag
    12541 agagccggcc accaccatgg tgagtgcgag cggaatctgc aggagggcca cgattttcag
    12601 aaacagggtg cccaattcga cgacggcctc atcactggtg aaggcgcgga gcaacgcgta
    12661 ggggaacaag aaaaacacca ctcccatggc cgacatcatg taggcggcca gccggtttgc
    12721 ttcccaggtt tcgagcttcg cccgagtgta tttgccggcg ccgatgctct gtcccaccat
    12781 cgtggctgct gcgatcgcaa agccatatcc cggaagaaac gacagggact cgatcgacaa
    12841 gccgacctgg tgggctgcgt aggcgacggt gccgtacatc aacacaatct tggtgtatag
    12901 caaaatgccg gcttgctgga cgacgcgctc acccgagacc ggtgccccca cgtgccagat
    12961 cgtgcgaatc aaatcgaatc ggaatcggtg ggagccgcgg agcataggcc gacagcgtgt
    13021 gaagaggtac aaacatccgg tcgcttccgc gatgccgacc gcaatggctg cgccattgat
    13081 gcccaaggcc ggcaggcccc agtgaccata gatcagggga atcgcgatgg caatgtggag
    13141 cagattcacc acgatcatgg cgtacatcgg cgttttcgtg tcgcccgtgc cctgcaagat
    13201 cgaagagagg acctgcagca ggatcgtgaa cggaatgagc aggaagatca ggttcgagta
    13261 ggggagcgcg aggcggatca cgtccgcttg cgccccgagc atttccatgg ccagccgatt
    13321 gaccagaagg ccgaagctca agagaccgag cgcaatgagg atggagagtc cgaggaaatg
    13381 acgcgcggca tggcctgcat cgtcgtaacg gcgggcgccc cacaattgcg ccatgaccac
    13441 attggatccc accgaaagac cggaaaccag ggtcgtagcg ataaaggcca gcaattgacc
    13501 gaggccgacg gcggcgatcg ggacggcccc caatcctcca acgagaaaga cggcgacgat
    13561 gccttcagcc cgttgcaaca ggctactgac ggtgacgggc agtgcgagcg tgagaaccga
    13621 gcgtctgatc tgtgcgatgc cctgtgccat gcggcatcat cctaacagaa ccgggctcgc
    13681 ttcgtggagt ttctcgccgg ctgggcggga ctggacaact cacgtcatgc ttcggtagcc
    13741 tgcacacaga ctatgctgat cttacctgag cctgccgcgc gctcctccta tcgtttatcc
    13801 aaatcgcgtt atctatcggg tctgcaatgt cataagcgtc tgtacctcga ggtgcatgct
    13861 ccggggttgg cgaccaaacc ggatgccgcc acgcaggcga ttctggatat gggcacggat
    13921 ctgggtgaac tggcgcgtca acgtttcccc ggcggccgtc tagtcacggc cgggtatcgt
    13981 caatctcagg aagccctggc gcaaacggct gagctgctac aggatccgac ggttccggcg
    14041 atcttcgaag gggcctttca gttcaaccag gttctggtcc gtgtggatgt cctgcaacgt
    14101 gtcggggtga atgagctggg gcaaccgacc tggcggctcg tggaggtgaa gtcttccacc
    14161 aaggtgaagg cgacgcacgt ggatgatctg acgatccaaa gctatgtcct ggaaggactt
    14221 ggactggtgc tggcggaaat ctgcctgatg catgtgaata cgcagtacgt ctttgacggg
    14281 cggtatctcg atctcgacca gctcttcggc cttcgttcac tgacggagac cgtgcgtccg
    14341 cgattaccgg aagtgccggc gcgactcgct gccatgcagg ccatgttgga aggcatgtcg
    14401 gctccggccg tgatgccaga tgaacattgt caaagcccct atgagtgtcc cttctggacc
    14461 cattgcatcg aacacaaacc ggctcgttgg atctatcatt taccgggaag tagcaagacg
    14521 gtcgtgcagc ttcgtgaatt gggcgttgag acgatcgacg agattcctga tcatgtcgca
    14581 ctcacgccgg tgcaacggcg ggtgaaagat aaccgggaat ggatcggcga agggctgcgg
    14641 tcggcgttgg agacgatcgt ctatcccgtc caccatctgg attttgagac cttcatgccg
    14701 gcggtgccaa aattcgtgga taccagaccc taccaggtta ttcccactca gtggtccaat
    14761 cacattgagc agtacgacgg cacgctcacg catgccgagt ttctgtgccg ggatgaacgg
    14821 gatcccaggg aagagttggc catcacgtta ttggaatcac tcggccagac gggcagcatc
    14881 tgcgtctatt cgagctatga acgatcggta cttgagcggc tggcggagga tttcccgtcg
    14941 ttacggaagg atctcaagcg ggtagtggcg aggctgtggg atctccacgt gatcatccgg
    15001 gatcactatt atcatcctgc ctttgaaggc agctactcca tcaaagccgt attacccgcc
    15061 gtcatgccgt ccctgagtta tgccgatctg gtgattcggg atgggggtgt cgcggcttgt
    15121 gaatacagcc gcatgatttt taccgtgacc gactggattg agaaggccca gattcaggag
    15181 gcactgctac gatactgtga acgcgatacc ctggccatgg tcgagttacg gagggaattg
    15241 aagcgacgag ccggttagtg cctgccccgc gtgcgaatcc atctcaccat ctgcagacag
    15301 gttgtaccca gtttttcatc cgccatcagt gcaattccgc ccaaggccaa cagcgtcatc
    15361 accagtgcgg catcggtttg ttcaaacatg tggccctcct gtgtggacca gagactaccc
    15421 tggcccggtg gaggcgaata gaccactaga ggaaggggac ggcacgatct tcggtaggtg
    15481 cagcggctct tcgtgcggcg attcgaagag agatgcctga caattcagcg aaacataagg
    15541 gcaagttcca gggaggggct ggttcgactc ccacaggcaa aaatcggggt gttggatcgg
    15601 gaatggcgga gagggtggga ttcgaaccca cggtcccttt caggacaacg gttttcg
//
LOCUS       DAOQDS010000083        14985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1707_length_14985_cov_20.546216, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000083 DAOQDS010000000
VERSION     DAOQDS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14985
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1707_length_14985_cov_20.546216"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PK589_16020"
     CDS             <1..222
                     /locus_tag="PK589_16020"
                     /inference="COORDINATES: protein motif:HMM:NF020534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098609 - cell-cell adhesion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin domain-containing protein"
                     /protein_id="HNJ21168.1"
                     /translation="GPKEYATLKNLAGKDWSNDIESVIVGSNATVRAYEDKDFKGTEI
                     AFAPGQRVPNLGKLDMANDIESLKITCGK"
     gene            complement(250..1164)
                     /locus_tag="PK589_16025"
     CDS             complement(250..1164)
                     /locus_tag="PK589_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21169.1"
                     /translation="MNRRWIVALAFALCTIPSLVFAESPPQSVHWGAIAFPDHDPTLT
                     LSTAVLDRFTQFDGAGRRYNDINQTMGFNFFTISWTKPLTQLPGWNLNLTAGGGPTRD
                     GPSRFLQNDVVHKLRGFDPVPVGEKRDANDFMLSGTMTRWFGLLGTSEVFFAGVGGAG
                     GSLYYEPYVQAGFRRLAILNLIPVLGDYLRVSGLARYGRPFSGAAFHQVAPQSYLAQA
                     SVGLGNYHHWKDCTPWEIELAVTVDSGLFVDHRGDALEERFVSLALRYSAFTFETWND
                     LINQKDYGPTFGARLTLDLFYMYDRWFH"
     gene            complement(1264..2379)
                     /locus_tag="PK589_16030"
     CDS             complement(1264..2379)
                     /locus_tag="PK589_16030"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNJ21170.1"
                     /translation="MNQTCTVHDPIPPQDAAQMEQRVEEHLTVHSRALTGLFLLAVLY
                     TLHFGQVIFLPMTIAFLLAVLFAPLLRRLRQWHLPEPLGAAILLALLVSGLVYGGSRL
                     AEPAAEWSTKVPVALRSVEYKIQPVKKSMQDVTKATELLSKATALDETKKVQQVEVKG
                     DAWPIKVFSLTGELMMGFATTVMLLYFLLSSGDLFLQKLVKVLPRLSDKKQAVEIVRN
                     MEEQLFLYLFTVTGINLALGTAVGIAMYLLGMPNPILWGVMAGLLTFIPYIGHIFGIV
                     VVMLVAALTFDDLGQMFLIGGVYWGLAILEGSFASPMILGRRLESNPVVLVLAIMIWG
                     WIWGVGGALLAVPLLAAFKIMCDHLEPLQPIGEFLGK"
     gene            complement(2376..4472)
                     /locus_tag="PK589_16035"
     CDS             complement(2376..4472)
                     /locus_tag="PK589_16035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ21171.1"
                     /translation="MRERDKDAIAIIPAYNEAGTIHHVASRTLHYVERVVVVDDGSTD
                     QTVSSLRELPVTVLRHANNLGKAAALRRGMQHAVAQGATAIITLDGDGQHDPAAIPAL
                     LAMHRRDPRAIVVAARRRHRHTIPRLRVLAQRIADFWISLAAGCTIEDSQSGFRLYPI
                     SAWTAAGWPCDAASGFVFESEILIEAGRSGIPIRSVPISAIYGRHLRQSHFQSGRDIA
                     AITRMVTDKILSDWLRAPGTTVRRRRALGPAVPSPDGAGTPQVLASAQKPRILFLAEA
                     VTLAHVARAVALARSLDPDQYEVHLACDRRYHHLFESVPATMHPIRTISGAQFQDRLR
                     RGDPLYSKEELHRYVEDDLCLFAHVRPDAVVGDFRLSLSVSARVAAVPYLTVTNAHWS
                     PFAHQHFVVPELAITERFGPRLGQALFTMMRPFVFAQQGHALNQVRRDYGLPALPYSL
                     PHQFTDADVTLYADVPELVPTYHRPPHHHYLGPVLWSPDSVPRWWDSLPDTGRMAYVT
                     LGTSGRADLLPLILDALEQLGIQALVSTAGRSIPGRLPPGTRVAQYLPGQAAAARADL
                     VICNGGSATVYQALAAGKPVLGIPNNLDQYLMMDYVRRSGAGDYLRAGTISRAAVTML
                     CDGLLGNPAFRDRAGRVQTAIEQNRTARAFDDHLRSVLGTGRSHQAPSKIRTPKVSAF
                     DSVPEESSPSNRASRR"
     gene            complement(4469..5191)
                     /gene="fabG"
                     /locus_tag="PK589_16040"
     CDS             complement(4469..5191)
                     /gene="fabG"
                     /locus_tag="PK589_16040"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNJ21172.1"
                     /translation="MSKRALVTGGSGGIGQAVCQVLGEAGFEVIVHGCTRMDRAEAVA
                     QAIRDKGGKAEALCFDVTDAAQTRQALDRLQPAGPIQVLVNNAGIYDDAPMAGMTNDQ
                     WHRVLDVSLNGFFHVTQPLLLPMIRTRWGRIVTITSVSGLIGNRGQVNYAAAKAGLHG
                     ATKALALELASRHITVNAVAPGIISTPASLDTFGAEAIERLVPMKRAGTVEEVAGLVS
                     FLVSDTAAYVSGQIISINGAQA"
     gene            complement(5188..5664)
                     /locus_tag="PK589_16045"
     CDS             complement(5188..5664)
                     /locus_tag="PK589_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21173.1"
                     /translation="MERRCDDIAELVPHRGTMCLLDSVETWDENHIVCRATSHLRPDN
                     PLRDGSGLRAVCAIEYGAQAIAAHATLLGSGHRQRAAEGVLASLRHVTTTQSHLDGLK
                     GALTILATLQLSHQQGRIYDVTVTADGQTLLTGRLSVMLEADHSGEARSVSQEGAA"
     gene            complement(5664..6461)
                     /locus_tag="PK589_16050"
     CDS             complement(5664..6461)
                     /locus_tag="PK589_16050"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HNJ21174.1"
                     /translation="MMEFWVISIGIAGPGLDGWAAARPILAGETEYRPAPLTPPNLKL
                     LSAAERRRCSQSARLAIAAAQDALLAGPLQGEAIATVFTSSDGDGAITQEICESLAGA
                     AREVSPTSFHNSVYNAPAGYWSIATRSQLSSTSVCAYDVSFAAGLLEAATYTTVERCP
                     VMLVASDLPFPRPLYGIRPVEESFAAALLMTPTSHPDACMRCEIALAPHQAITAFPAT
                     IPESLRSNPAARSLPLLALLARQAADTVRLEYTDESDLVIDCKPEGH"
     gene            complement(6458..7648)
                     /locus_tag="PK589_16055"
     CDS             complement(6458..7648)
                     /locus_tag="PK589_16055"
                     /inference="COORDINATES: protein motif:HMM:NF006618.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNJ21175.1"
                     /translation="MMAPLTVTAMATANPLGLGLAETWQALRTERQGLRPNDFEDADI
                     KTWIGRIEGLETEPITGELADFDCRNNRLALVGLRQDGFEQAVASARQRYGPDRIGVL
                     IGTTTSGILETEWAYRRRAAGGELLPLTVRYRHTQNLFSVSDVVRRHLKLTGPVASIS
                     TACSSSAKVFASASRWMAAGLCDAVVVGGVDSLCFMTLYGFASLGLLSPRPCRPCDEH
                     RDGLSIGEAAGFLLLERETTRDSGLRFLGYGESSDAHHMSTPHPEGLGAVLAMERALD
                     RAELKPIDIDYINLHGTGTPANDLAEDRAIWKTFGERTPCSSTKERTGHTLGAAGITE
                     AILSLLCLRHEFIPGMVRIEQPDPQLRSGIMLRSESRPLRTVISNSFGFGGSNCSLIF
                     GKRP"
     gene            complement(7645..9966)
                     /locus_tag="PK589_16060"
     CDS             complement(7645..9966)
                     /locus_tag="PK589_16060"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNJ21176.1"
                     /translation="MTLHARPALFLWMLLVFISGGIVANMTMTTDLTALLPRSADHRQ
                     ELLVSQLRDGVAARLILIGLSGAEGEALAQASRTIARKLRDTGLFASVNNGDPADFTI
                     VREVLMRHRYLLSAAVIPEHFTATALREALERQLQFLASPAGALMKTALPSDPTGEVP
                     HILAAFTPGEGPAMHQGVWFSRDGTRALLVAETSAPGFALDQQESAVQAVRTAFTSAG
                     LPEHAHLVMTGPGVFAVESRATIQRDSWRLSVIAITVVTILLFLVYRSVTPLLLSLLP
                     VLTGLLVGVAAVQTWFGFVHGITLGFGATLIGEAVDYPAYVFTQGIAGERLQQTLSRI
                     WPTLKLAVLTTVFGALSLLFSSITGLAQLGVLAFVGVLVAGFVTRWILPALPPGVIRS
                     APHQLLPMSWLPALASVNRIRWTIWVAALLALATLAMRHENIWDNDLANLSPIASPSK
                     TLDEQLRKDLGAPDVRYVLIVEGSSKEEVLQRSESAELPLRRLVDDGLLAGFDLPSLY
                     LPSDASQRKRQAALPSSDVLAASLAEAVKGLPFRAGLFEPFLHDVEMARTSPLIDIVN
                     LQQTPFFLKIRSLLVHTDDAWAGLVPLRHVTSPSGLADRLAREAGPGLTFLDLKSEAG
                     RLVNGYRHESLRLTALGVVAMTILLWWGLRDMALVSRVLLPPLLAILFVVTLLTVIGE
                     RLSLFHLVSLLLVLGIGLNYALFFNRPFSDEEEKRRTWLSLTVCMAATLSAFGALAFS
                     RTPVLHAIGLTVGLGAAGSLLIAAILGQQRVSQ"
     gene            complement(9963..10562)
                     /locus_tag="PK589_16065"
     CDS             complement(9963..10562)
                     /locus_tag="PK589_16065"
                     /inference="COORDINATES: protein motif:HMM:NF039988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HNJ21177.1"
                     /translation="MKVPITLSPLILLCSLWAWLPASAEPAMGAEPFDVPGLMELLAK
                     VSSRQDRFTETKTIAMLTQPVVLKGTLAYVRPDRVEKHITSPYTEDLTVQGDRLTLSS
                     PKGTKQIAVDSHPLIRSFIEAIRASLAGELAVLRRLYHIKFQGTRQQWELTLRPLDAQ
                     AAEYVASIILRGEGDRLTTVDIRETGGDRSLMEIDGPVS"
     gene            complement(10519..>11220)
                     /locus_tag="PK589_16070"
     CDS             complement(10519..>11220)
                     /locus_tag="PK589_16070"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21178.1"
                     /translation="FRHFHAFASTIHDRVYLLSGRHRYFDIHTEGASALHAVLAQNRG
                     CILLGSHLGSFEVLRAQGIFDHKLPITVIMHEAAASNLNEVLHNLRPEIRDRIIPPGT
                     ADTMLAVKERLDRGEIVGILGDRLFNDEKGMACEFLGKTRMFPEGPFLLAALLQVPIV
                     LFFGLYQGGRRYHLYFEPLADTIPVNGARRSETLRPWIERYVSRLEHYCRVSPYNWFN
                     FYEGPDHAQPSHPAV"
     assembly_gap    11221..11320
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11488..12909)
                     /locus_tag="PK589_16075"
     CDS             complement(11488..12909)
                     /locus_tag="PK589_16075"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ21179.1"
                     /translation="MMMQSIDSQMAPASPARRMHQPRAAAGYPLVGHHSVTSPLAWRR
                     DRAHSVHEFLCAAISLAAQLPRKHFAINLCRDRYHFAVGFAAALIARQISLLPTCRAA
                     EPLQQLARQYPGAYVLTDHDEGDIPIPRFSLLREMEQGVGPKDVPVIPADQIAAIAFT
                     SGSSGFPQAHAKSWGSLVMGARALGQQFGLTQSSSRVAVGTVPAQHMYGLETTIMLPL
                     QWGWSVHAGTSILPADLRTDLEQLESPAWLMTTPFHLKAFLRERTNLRGLEGIISATM
                     PLDRSIALSAERLWHVPVHEIYGCTEGGMLASRRTTAGEAWTLCHDLRMRHAEDTTWV
                     MGGHVGAWLQLSDRITLRSQREFLLLGPHRDLVKVAGKRASLTALNAILNRIPGVVDG
                     TFYWPDPGQTGTGRLRAFVVAPGMTRAAILAELRKRIDPVFLPRPLYVVDQLPRNTTG
                     KLPHEHLKTFARHIMHPQQHVHE"
     gene            complement(12906..13187)
                     /locus_tag="PK589_16080"
     CDS             complement(12906..13187)
                     /locus_tag="PK589_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNJ21180.1"
                     /translation="MAFSSQSQVGADGALEQELATLIVEALRLEVLPETIDPSSALFG
                     EGLGLDSIDMLEIALLVSKRYGVELRSENKAALASLRALANHIAQHRIR"
     gene            complement(13189..14010)
                     /locus_tag="PK589_16085"
     CDS             complement(13189..14010)
                     /locus_tag="PK589_16085"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNJ21181.1"
                     /translation="MNTVSMRHSATNEGLGGRRPADAPLTLRFLVTFDDGPHPNTGKV
                     LRHLARNPVQPHIKGIFFVQTRHPKRGGSSDGRAMLAIEQAEGHLLGLHTGTTKGHLS
                     HTRMSPAELERSLLNGKEDLDQITGKPTMLVRPPYWLFNASTFDCYGRHRLHMLLSDV
                     KAFDGVNWGIHLLRRLNFRSQLAGLHARFLRGTLPVVNHTIPIVVTFHDTNSFTAEHL
                     GDYLSLLVEEAARLGLPLHSKPFIDDPDELLAAALRCAKTSTGTERNPALIQEAQ"
     gene            complement(14029..14301)
                     /locus_tag="PK589_16090"
     CDS             complement(14029..14301)
                     /locus_tag="PK589_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21182.1"
                     /translation="MSRGWALGIAVLLTGCGPHYTIPNYDTNEIEKVISSGRSPQGCI
                     ENLKEDAKNLNVDVRLTDVQQQPSSGPISWIYTNTYMCTGKVVAKR"
     gene            14525..>14985
                     /locus_tag="PK589_16095"
     CDS             14525..>14985
                     /locus_tag="PK589_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNJ21183.1"
                     /translation="MPAKKGSTARPSAAANQGQPTEEFEKLGVFYLGRPYALATKQSK
                     PGWLLYDSKDLVTHAVCVGMTGSGKTGLCVGLLEEAAIDGIPAIVIDPKGDLSNLLLT
                     FPQLRGEDFAPWINEDDARKKGLSPADYATQQAALWQKGLGEWGQSGERIQ"
BASE COUNT         3054 a         4356 c         4686 g         2789 t
ORIGIN      
        1 ggtcccaaag agtatgccac gttgaagaat ctggccggga aggattggag taatgatatt
       61 gaaagtgtga ttgtcggttc caatgccacg gtgcgtgcct atgaagacaa agacttcaag
      121 gggacggaaa tcgcctttgc gccgggacag cgtgtgccga atctgggcaa gctggatatg
      181 gcgaacgaca tcgagtcgct gaagatcacc tgcggaaagt gagacagggg aagagaaccg
      241 gtccggcctt cagtggaacc agcggtcgta catatagaac aaatcgagcg tcaagcgggc
      301 gccgaaggtc gggccgtaat ctttctggtt gatgaggtcg ttccacgttt cgaaggtgaa
      361 cgcggagtag cgcaacgcca gtgacacaaa ccgttcttcc agcgcgtccc cccggtggtc
      421 cacgaacaga ccggaatcga cggtcacagc cagttcaatt tcccacgggg tacaatcttt
      481 ccagtggtga tagttgccta agcccaccga cgcctgggcg aggtacgatt gcggggcgac
      541 ctggtgaaac gccgcgccgc tgaatggccg gccatatcgt gccaatccgg agacccggag
      601 atagtcgccg agcacgggaa tcagattgag aatcgccagt cggcgaaatc ctgcctgaac
      661 gtacggctca taatataagg atccgcccgc cccgcctacg ccggcgaaaa acacctcgct
      721 ggtccccaag agcccaaacc agcgcgtcat ggtcccgctc agcataaaat cattggcgtc
      781 acgtttttcg ccgaccggca ccgggtcgaa gccgcgcaac ttatggacga catcgttttg
      841 caagaaccgg ctcggcccgt cacgggtggg gccgccgcct gcggtgaggt tgagattcca
      901 gccgggcaat tgggtaagcg gtttcgtcca gctgatggtg aaaaagttga accccatagt
      961 ctggttaatg tcgttatagc gtctccctgc tccgtcgaat tgcgtaaacc gatccaggac
     1021 ggcggtactc agggtcaggg tggggtcatg gtcgggaaac gcgatcgcgc cccaatgtac
     1081 gctttggggc ggggattcag cgaagaccag actgggaatt gtgcagagcg cgaaggcgag
     1141 cgcaacgatc catcgacgat tcataggcgc gcgcatcctg ccagtatcgc catcacgttg
     1201 gcaactgttc tttgcggagg aacgatcaag gaaggcacga ttggtagctg tcgagtgcgc
     1261 cggctacttg cccagaaatt cgccgatggg ttgcaggggt tcgaggtgat cgcacatgat
     1321 tttgaatgca gcgagtaggg ggacggccag gagcgctccg ccgacgcccc agatccagcc
     1381 ccagatcata atagccagga ccaggacgac cgggttgctt tccaagcgcc gccccaaaat
     1441 catcggcgac gcgaaggatc cttccagaat ggccaggccc caatagacac cgccgatgag
     1501 aaacatttgt ccgagatcgt cgaacgtcaa cgccgccacc aacatcacca ccacgatgcc
     1561 gaagatgtgg ccgatgtacg gaatgaaggt taagaggccg gccatgaccc cccacaggat
     1621 cgggtttggc attccgagca ggtacatagc gatgccgacg gcggtgccca gtgcgaggtt
     1681 gatcccggtg acggtaaata ggtagaggaa gagctgttct tccatgttcc gcacaatttc
     1741 cacggcctgc tttttgtcgc tcaggcgcgg caataccttc acgagctttt gcaggaagag
     1801 atcgcccgac gagagcagaa aatacagaag catgaccgtc gtcgcgaagc ccatcatgag
     1861 ctcgccggtg aggctaaaga ccttgatggg ccaggcatct cctttcactt cgacctgctg
     1921 cacctttttt gtctcatcca aggcagtggc cttcgatagc agttcggtgg ctttcgtcac
     1981 atcctgcatc gattttttga ccggctgaat tttgtattca accgaccgca atgccaccgg
     2041 cactttggtg ctccactccg ctgccggttc cgccagacgc gagcctccat acacgaggcc
     2101 gctgactaac agtgcgagga gtatcgctgc cccgagaggc tccggcagat gccactgccg
     2161 caggcgacgc aggagggggg caaacagcac cgccaacaag aacgcaatcg tcatgggcag
     2221 gaagatcacc tgtccgaaat gcaacgtata gaggacggcc agcaggaaga ggccggtgag
     2281 cgccctggag tgcaccgtga gatgttcttc cacccgctgc tccatttgag cggcatcttg
     2341 gggcgggatt gggtcatgga cggtacaggt ctgattcatc gccgactcgc ccgattggat
     2401 ggagagcttt cttcgggaac agaatcgaag gccgacacct tcggcgtccg gatcttggac
     2461 ggagcctgat gagacctgcc cgttcccaat acgctcctca aatgatcgtc gaatgctctc
     2521 gctgtgcggt tctgctcgat cgcggtttgt acccggccgg ctcgatcgcg gaaggcgggg
     2581 ttgcctaaca agccgtcaca cagcattgtc acggcggcac gggagatggt ccctgctctg
     2641 aggtagtcgc ccgcgccgct ccggcggacg tagtccatca ttaagtactg atcgaggttg
     2701 ttgggaatac ccaacaccgg cttgccggcc gccagggctt gatagacggt cgcgctccct
     2761 ccattacaaa tgaccagatc cgctcgcgcg gcggctgctt ggccaggcag atactgtgcg
     2821 acccgcgtac cgggtggaag ccgtccgggg atggagcgcc cggcggtgga caccaaggcc
     2881 tggataccaa gctgctccag tgcgtccaga atgaggggca agaggtcggc ccgtccggac
     2941 gtgccgaggg tgacgtaggc catccgtccc gtatcaggca atgagtccca ccatcgagga
     3001 accgagtccg gcgaccagag aaccggaccc agatagtgat ggtgcggcgg tcgatgatag
     3061 gtcggaacaa gctccggcac atcggcgtag agcgtcacgt cggcgtctgt gaactggtgc
     3121 ggcaggctgt acgggagtgc cggcaatcca tagtcgcggc gaacctggtt caaggcatgt
     3181 ccctgctggg cgaaaacaaa tggacgcatc atggtgaaga gtgcttgtcc caggcgcggg
     3241 ccgaagcgct ctgtgatggc caattcggga acgacgaaat gctggtgtgc gaacgggctc
     3301 caatgggcat tggtcaccgt gaggtaggga acggcagcga ctctggcact cacggacagc
     3361 gacagacgaa agtctccgac caccgcgtcc ggcctcacat gagcaaacag gcacagatcg
     3421 tcttcgacgt atcggtgtaa ttcctccttg ctatagagcg gatcccctcg acgtaggcga
     3481 tcctgaaatt gcgcgccgct gatcgttcga atcgggtgca tcgtggcagg caccgattcg
     3541 aagagatggt gatagcgccg atcacaggcg agatgcacct catattggtc aggatcaaga
     3601 ctgcgggcca aggccaccgc acgggcgaca tgggccagcg tgaccgcctc cgccagaaag
     3661 agaatgcgag gcttttgtgc cgacgccagc acttgaggag ttcccgctcc atctggagag
     3721 ggaacagccg ggccgagagc ccgtcgccgg cggacggttg tgccgggcgc cctcagccag
     3781 tcggagagaa ttttatcggt gaccatccgg gtgatggcgg caatatcgcg tccgctttgg
     3841 aagtggcttt gtcgcagatg ccgtccatag atggcagaaa tggggaccga tcggatggga
     3901 atgccagacc gaccggcctc gatcaggatt tcgctttcaa acacgaatcc ggaagccgca
     3961 tcgcaaggcc acccagccgc cgtccaggcg gaaatcggat agagccgaaa tccagactga
     4021 ctgtcttcga tcgtacagcc tgctgccagg gagatccaga agtccgcaat ccgctgtgca
     4081 agtacgcgga gtcgcggaat cgtgtggcga tgccggcgcc tcgctgcgac gacgattgcg
     4141 cgcgggtccc ggcggtgcat ggcgagtaag gcggggatgg cggctggatc atgctggccg
     4201 tccccgtcga gtgtgataat cgccgtagcc ccttgcgcga cggcatgctg catccctcgt
     4261 cggagcgcgg cggccttgcc caaattgttc gcatgtcgca ggaccgtcac gggcaattcc
     4321 cgcagcgacg acaccgtctg atcggttgag ccatcatcca ccacgacgac ccgctcgacg
     4381 taatgcaaag tccgggaggc gacatggtga atcgttccgg cttcattgta ggcggggatg
     4441 atggcaatag cgtccttgtc ccgttccctc atgcctgggc tccattgatc gagatgatct
     4501 ggccggatac ataggccgcc gtatcggaga cgaggaacga gacgaggccg gccacttcct
     4561 caacagtgcc ggctcgtttc atcgggacca gtcgctcgat ggcttctgcg ccgaatgtgt
     4621 cgagcgacgc cggcgtggag ataatgccgg gcgcgacggc attcacggtg atgtgacgtg
     4681 aggcgagttc cagcgccagg gctttcgtgg cgccatggag accggctttc gccgcggcgt
     4741 agttcacctg gccgcgattg ccgatgagcc cgctgacaga cgtgatggtg acgatacgcc
     4801 cccaccgggt ccggatcatg ggcagcagga gtggctgcgt cacatgaaaa aatccgttca
     4861 gtgacacatc gagcaccctg tgccactggt cgttcgtcat ccccgccatc ggcgcgtcgt
     4921 catagatacc ggcgttgttc acgaggactt gaatggggcc ggctggttgc aggcgatcta
     4981 aggcctggcg ggtttgcgct gcatccgtga catcgaagca cagggcctcg gccttcccgc
     5041 ccttgtcacg gatggcctgc gccacggcct ccgcccgatc catgcgggtg catccgtgga
     5101 caatgacctc gaatccggcc tcgcccagca cctgacagac ggcttgcccg atgccgccgc
     5161 tgcctcccgt caccagcgca cgcttactca tgcggctcct tcctgtgaga cggatcgagc
     5221 ctcgccagaa tgatccgcct ccagcatcac ggaaaggcgg ccagtcaaca gcgtttgtcc
     5281 atctgcggtc accgtgacgt cgtagatacg gccttgctgg tggctcaact gcagcgtcgc
     5341 gaggatcgtg agcgcaccct tcagtccatc caagtgagac tgcgtcgtgg tgacgtgacg
     5401 gagcgaagcc agcacacctt ccgccgcgcg ttgtctatgc ccactcccga gcaacgtcgc
     5461 atgcgcggca atggcctgcg cgccgtattc gatcgcgcag acggcgcgaa ggccgctgcc
     5521 gtctcgtaag ggattgtccg ggcggaggtg gcttgtggca cggcagacga tatggttttc
     5581 gtcccatgtt tcgactgagt cgagcaggca catggttccg cgatgaggaa ccagctcggc
     5641 gatgtcgtca cagcggcgtt ccatcaatga ccctccggtt tgcagtcgat cacgaggtcg
     5701 ctctcgtccg tgtactcgag ccgcacggtg tctgcggcct gtcgggccag cagggccaac
     5761 aggggtaagc tgcgcgccgc cgggttgctg cgcagcgact cgggtatggt agcgggaaag
     5821 gccgtgatgg cctgatgcgg ggccagcgcg atctcgcacc gcatgcacgc atcgggatgg
     5881 cttgtcggag tcattaaaag cgccgcggcg aaactttctt ccacggggcg aattccatac
     5941 agaggcctgg ggaagggcag atcggacgcg accaacatca ccgggcagcg ctcgacggtg
     6001 gtataggtgg ccgcttcgag gagtccggcg gcgaacgaca catcgtaggc gcagacactc
     6061 gtcgatgaga gttgcgagcg ggttgcaatg ctccaatagc cggccggggc gttgtagacg
     6121 gagttatgaa acgacgtggg ggatacctca cgtgcggcac ccgccagtga ttcgcatatc
     6181 tcttgtgtga tggccccgtc accatccgaa gacgtaaaga cggtggcgat cgcctctccc
     6241 tggagaggtc ctgccagcaa ggcgtcctgc gccgccgcga tcgcaagacg tgcggattgg
     6301 ctacagcggc ggcgttcggc ggcggacaac agcttgagat taggaggtgt gagaggcgca
     6361 ggccggtatt cggtttcacc ggccaggatg ggtcgagcag cagcccatcc gtccagcccg
     6421 gggccggcga tcccgatgct gatcacccag aactccatca tggcctcttt ccgaaaatga
     6481 gactgcaatt gctcccaccg aaaccgaatg agttactgat cacggtgcgc aagggacggg
     6541 attcgcttcg aagcatgatg ccgctgcgga gttgcggatc cggttgctcg atccgcacca
     6601 tgcctggaat aaattcatgc cgcaggcaga ggaggctgag aattgcttcg gtgattccgg
     6661 cagcgccgag ggtatgcccg gtcctctcct tcgtcgaact acacggcgtt ctttctccga
     6721 acgttttcca gatggcccgg tcttccgcga gatcattggc tggcgttccg gtgccatgca
     6781 gattgatgta atcgatatcg atgggtttca actccgcccg gtcgagcgcg cgttccatag
     6841 ccaagacagc acccaggcct tccgggtgcg gcgtggacat atggtgggcg tcgctgcttt
     6901 cgccgtaacc cagaaaccgc aaaccgctgt cgcgggtggt ctcgcgttcc agcaaaagga
     6961 atcccgcggc ttccccgatg gaaagcccgt ctcgatgttc gtcgcaggga cgacaggggc
     7021 gaggcgacag caggcccagg gaggcaaacc catagagcgt catgaagcac aaactgtcca
     7081 cgccgcccac cacgacggca tcacagagcc ccgcggccat ccagcgcgag gcgctcgcga
     7141 agactttggc gcttgaggaa caggccgtgg aaatcgatgc gaccgggccg gtgagtttca
     7201 agtgacgccg cacgacatcg ctcacggaga acaaattctg tgtgtggcga tagcgcacag
     7261 tcagcgggag caattcaccc ccggccgcgc ggcgtcgata ggcccattcg gtttcgagaa
     7321 tgccggaggt cgtcgtgccg atgagtacgc cgatgcgatc cggcccgtac cgctgccgcg
     7381 ccgaggcgac cgcctgttca aacccatcct gtcgtaaccc gaccagcgcc aatcgattgt
     7441 tgcggcaatc gaaatcggcg agttcgccgg taatcggttc cgtttcgagt ccttcgatcc
     7501 ggccgatcca ggtcttgatg tccgcatctt cgaagtcatt cggccgcaaa ccctgtcgct
     7561 ccgtgcgcag cgcctgccac gtctccgcca gccccagtcc gagcgggttg gcagtggcca
     7621 tggcggtgac ggtcaatgga gccatcattg cgacacccgt tgttggccga ggatcgcggc
     7681 aatgagtaag gaccctgccg cgccgagacc aacggtgagc ccgatggcat gcagcacggg
     7741 tgtgcgagaa aaggccaggg ccccgaacgc cgacagggtg gccgccatgc agaccgtgag
     7801 cgaaagccag gtccgtcgtt tctcctcctc atctgagaag ggccggttga agaacagcgc
     7861 gtaattgagc ccgatgccca acacgagcaa gagtgacacc agatgaaaga gggagaggcg
     7921 ttcgccgatc acggtgagca gggtgacgac gaacaggatg gccagtagcg gtggtaacag
     7981 gacgcggctc accagcgcca tgtcccgcag cccccaccac aggagtatcg tcatggccac
     8041 cactccaagg gcggtcagcc gtaaggattc atgccggtac ccgttgacca gccgccccgc
     8101 ttcggatttc agatcgagga aagtgagtcc gggccccgcc tcccgagcaa ggcggtcggc
     8161 cagaccggag ggagaggtca cgtggcgcag gggcacgaga cccgcccacg catcatcagt
     8221 gtgaaccaga agcgaccgga ttttgagaaa gaagggcgtc tgttgaagat tgacgatatc
     8281 gatcaatgga ctcgtgcggg ccatctcgac atcgtgcaaa aacggctcga acagtccggc
     8341 cctgaaggga agtcctttca ctgcctcagc caatgatgca gccagcacat cggaagaagg
     8401 cagtgcggcc tgccgttttc gttgagaggc atccgacggg agatacagac tcggcaggtc
     8461 gaacccggcc aacagtccgt cgtccaccaa gcggcgcaga ggcagttcag cggattcact
     8521 gcgttgcagg acctcttcct tgctcgagcc ctcgacgatc agcacatacc gaacatccgg
     8581 cgcgccgagg tcttttcgca gttgctcatc gagggttttt gacggcgatg caatcggact
     8641 caaattagcg aggtcgttat cccagatgtt ttcatgtcgc attgccaggg tggccagggc
     8701 cagcagggcc gccacccata tggtccagcg aatgcgattc accgatgcca gagcaggcag
     8761 ccatgacatc ggaagcagct ggtgtggagc ggaccggatg actccgggcg gcagggcggg
     8821 aaggatccag cgcgtcacga atccggctac gagaaccccc acgaatgcca gtacgcccaa
     8881 ctgcgccagg ccggtgatac tcgaaaacag caagctgagc gcgccgaaga ccgtggtcag
     8941 gacggccagt ttcaacgtcg gccatatcct cgacagggtc tgctgcagtc gttctcctgc
     9001 aatgccctgt gtgaagacgt aggcggggta gtcgaccgct tctccaatca gtgtggcgcc
     9061 gaaacccagt gtgatgccat ggacgaaccc gaaccaggtt tgtaccgccg ccactccgac
     9121 gagcaagccg gtcaacaccg gaaggagact cagcaggaga ggcgtcaccg accggtagac
     9181 gagaaacagc agaatcgtca ccactgtgat ggcgatgacg gacagtcgcc aagagtcgcg
     9241 ctggatcgtg gcgcgggatt ccaccgcgaa tacgccgggg ccggtcatga ccagatgggc
     9301 atgttccggc aggccggctg atgtgaatgc cgtccgcacg gcctgcactg ccgactcctg
     9361 ctgatcgagg gcgaaccctg gcgcgctggt ttcagcgacg agcagggctc tggttccgtc
     9421 gcgcgaaaac cagacgccct ggtgcattgc cggtccctcc cccggggtaa aggccgccag
     9481 tatgtgaggc acttcaccgg tgggatcgga gggcaaggcg gtcttcatca gcgcaccggc
     9541 cggagaggcg agaaattgca gctgtcgttc caatgcctcc cgcaatgccg ttgccgtgaa
     9601 atgctccggg atgacggccg cactcagaag gtaccgatgg cgcatcagca cctcacggac
     9661 gatggtgaag tcggcggggt ctccgttgtt taccgaggcg aagaggcccg tgtctcgtaa
     9721 tttgcgggct atggttcggc tggcttgggc cagtgcctcg ccctcggcgc ccgagaggcc
     9781 gatcaggatc agtcgggccg cgacgccgtc ccggagttgc gacaccagca gttcctgccg
     9841 atggtcagcg gaacggggta gcaaggcggt caagtcggtg gtcatcgtca tgttggcaac
     9901 gattcctccg ctgataaaca ccagcagcat ccagaggaac agggccggtc gggcatgcag
     9961 ggtcacgaga ccggcccatc gatctccatc aaggaccggt cccctccggt ttcccgtatg
    10021 tcgaccgttg ttaaacgatc gccctctcct cgaaggatga tcgaggccac gtattccgcc
    10081 gcttgcgcat ctaacggccg cagcgtgagt tcccactgct ggcgggtgcc ctgaaacttg
    10141 atgtggtaga gccgccgcaa tacggcaagt tctcccgcca gactggcccg gatcgcttcg
    10201 atgaaggagc gaatcaacgg gtggctatca acggcaatct gctttgtgcc cttcggtgag
    10261 gacagcgtca gccgatcgcc ttgcaccgtg agatcctccg tatagggcga ggtgatatgt
    10321 ttctcgacgc ggtctggacg aacataagcc agcgtgcctt tgagcaccac cggctgagtc
    10381 aacatggcga tggttttcgt ttccgtgaaa cggtcctggc gggacgagac cttggccagc
    10441 agttccatca gtccgggcac gtcgaatggc tccgctccca tggcgggttc agcggacgcc
    10501 ggcagccagg cccacaggct acacagcagg atgagagggc tgagcgtgat cgggaccttc
    10561 ataaaagttg aaccagttat acggagatac acggcagtaa tgttccagcc ggctgacgta
    10621 tcgctcgatc caagggcgta gggtttccga tcggcgggcg ccattcacag gaatagtgtc
    10681 ggccaagggc tcaaaataca ggtggtatcg gcggcccccc tggtagagac caaaaaacag
    10741 cacgatcggc acctggagca gcgcggcgag cagaaatggc ccttcgggga acatgcgggt
    10801 tttgccgagg aactcgcagg ccatgccttt ttcatcgttg aacaggcggt ctccgagaat
    10861 ccccacgatt tcccctcgat ccaatcgttc tttgaccgcc agcatcgtgt cggcggttcc
    10921 aggcggaata atccggtccc ggatctcggg gcgcaggttg tgcagcactt cgtttaagtt
    10981 ggaggctgcg gcttcgtgca tgatcaccgt gatcggcagc ttgtgatcga agatgccttg
    11041 cgcgcgcagg acttcgaaac tgccgaggtg agaacccagc aaaatgcagc ctcgattctg
    11101 ggcgagaacg gcatgtaatg ccgaagcgcc ttcggtgtga atatcgaagt agcgatggcg
    11161 tccggacagc agatacaccc ggtcatgaat cgtcgaggcg aaggcgtgga agtgccgaaa
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn accgagctca gttcgagggg
    11341 gccaggtttc ataccggctt cttgttcaag gatgctcagc aattcggtta agagcaccac
    11401 tcccgggacg agagggttgc caggaaaatg cccggccaaa gccgggtggt cccggcggat
    11461 ctgtcgcggt ttctctgtgc gcatgcgtta ctcgtgaacg tgctgttggg gatgcatgat
    11521 gtgtcgggca aaggttttca aatgttcgtg tggtaatttg ccggtggtgt tcctggggag
    11581 ctgatccacc acatataacg ggcggggaag aaagaccggg tcgatacgtt tccggagctc
    11641 ggcaagaatc gctgcacgag tcatccccgg cgcgaccacg aaagctctca atctgccggt
    11701 gcctgtttgt cctgggtcgg gccaataaaa cgtgccgtcc accacaccgg gaatgcggtt
    11761 caggattgca ttgagcgccg tcaaggaggc gcgttttccc gctaccttca cgaggtcacg
    11821 gtgtggcccc aggaggagga attcccgctg actgcggagc gtgatgcggt ccgacagctg
    11881 gagccaggct cccacatgtc cgcccatgac ccaggtcgtg tcttcggcat gccgcatccg
    11941 taaatcgtgg cacagcgtcc aggcttcacc ggccgttgtg cggcgagacg cgagcatccc
    12001 tccttccgtg catccataga tttcatgtac gggcacatgc cacagccgtt ctgcgctgag
    12061 cgcgatcgac cggtccaggg gcatcgtggc cgagatgatg ccctcaaggc cgcgcaggtt
    12121 cgtgcgttcg cggagaaacg ccttgagatg gaagggcgtt gtcatcaacc aggccggcga
    12181 ttccagttgc tccaggtccg tccgaaggtc cgcggggagg attgaagtgc cggcgtgcac
    12241 actccatccc cattgaagcg gcagcatgat cgtggtttcc aacccataca tgtgctgggc
    12301 agggacggtt cccacagcca ccctcgacga agactgcgtc aggccgaact gttgccccaa
    12361 tgcgcgcgcg cccatgacca gggatcccca gcttttcgca tgagcctgag ggaacccgct
    12421 actgccggag gtgaacgcga tggcggcgat ctgatcggcg ggaatcaccg gaacatcttt
    12481 cggaccgacc ccctgctcca tttctcgaag gagggagaag cgaggtatgg ggatatcccc
    12541 ctcgtcatga tcggtcagca catatgcacc gggatactgt cgcgcgagtt gttgaagggg
    12601 ttctgccgcg cggcaggtgg gcaacagact gatctgacgt gcgatcaagg ccgcggcaaa
    12661 tcccacggcg aagtggtagc gatcccggca aagattgatg gcaaagtgtt tgcgcggcaa
    12721 ctgcgccgcg agacttattg cagcacacag aaactcgtga acggaatgtg cacgatcacg
    12781 gcgccaggcg agcgggctcg ttacggaatg atgtcccacg aggggatacc cggctgccgc
    12841 tctcggctgg tgcatgcgcc gggcggggga ggccggagcc atctggctgt cgatcgattg
    12901 catcatcatc gaatccgatg ttgggcaatg tgattcgcca atgcccggag cgaagccagg
    12961 gccgctttat tctccgagcg caattccacg ccataacgtt tggataccaa cagggcgatt
    13021 tccagcatgt cgatggaatc cagccctagc ccctcgccga agagtgccga ggacggatcg
    13081 atggtctcgg gtaacacttc caagcggagt gcctccacga tgagggtggc cagttcctgc
    13141 tccaacgcgc cgtcggcgcc cacctgggat tgcgaggaaa acgccatatc actgcgcctc
    13201 ctggataagg gccggattgc gctccgtacc cgtgctggtt ttggcgcacc gaagcgccgc
    13261 ggctaataat tcgtcgggat catcgatgaa gggcttggaa tgtaagggga ggcccaaccg
    13321 cgcggcctcc tcgaccagaa ggctgagata gtcgccgagg tgttctgccg tgaaggagtt
    13381 cgtatcgtga aaggtcacca cgatgggaat ggtgtgattc accaccggca acgttccccg
    13441 aagaaagcgg gcatgtaacc cagccagctg cgagcggaaa ttcagccttc gcagcaggtg
    13501 gatgccccag ttcaccccgt cgaaggcttt gacatcggac aacagcatgt gaagccgatg
    13561 ccgtccatag cagtcgaagg ttgacgcgtt gaacagccag tatggagggc ggaccagcat
    13621 cgtcggcttg ccggtgatct ggtcgaggtc ctctttgccg ttgagcagtg accgctcgag
    13681 ttcagccggc gacatgcgag tgtgactgag gtgccctttc gtggtccccg tatgcaaccc
    13741 caaaaggtgc ccctcggctt gttcaatcgc cagcatggct cgcccatcgg atgagccccc
    13801 tcgcttcggg tggcgcgtct gcacgaaaaa tattcctttg atgtgcggtt gcaccggatt
    13861 gcgggcgagg tgccgcagga ctttcccggt gttgggatgg ggcccgtcat caaaggtcac
    13921 caggaagcgc agggtcagag gcgcatctgc aggccgtcgc ccgcccaacc cctcgtttgt
    13981 cgctgagtgc cgcatcgata cggtgttcac gtgtcagcct ttcgtgaatt atcgtttggc
    14041 caccactttc cccgtgcaca tgtacgtgtt cgtgtagatc caggaaatgg ggcccgagga
    14101 cggctgctgc tggacgtcgg tcaggcgaac gtccacgttc aaattcttgg catcctcctt
    14161 gagattttcg atacatccct gaggagagcg gccgctggaa atcacctttt cgatttcatt
    14221 cgtgtcgtaa tttggaatgg tgtagtgagg cccgcatccg gtcagcaaga ctgcgattcc
    14281 aagtgcccac cctctgctca tgtccaatct cccctcgttg acggtaaaaa agtccggcct
    14341 gtcagggttt gactgtatga aactgagacg aagacccaca ctgggggcct ccctagtttg
    14401 gccctaggag cccccgagtt tgctgaggcg aatgccggtt ccgttgcttg cggacatttc
    14461 tgtcaatcaa tacaatggcc tcgaccaatc cggccacacg tgcattgcca tgcgaggagg
    14521 aactatgcca gccaagaagg gatcaacggc tcgcccgtct gccgcagcga accaagggca
    14581 accgacggag gagtttgaga agctcggggt cttttatctc ggccggccct atgcgctcgc
    14641 gacgaagcaa tccaagccgg gatggctgct gtatgattcg aaggatctgg tgacgcatgc
    14701 cgtgtgtgtg ggcatgacgg ggagcgggaa gacgggcctg tgcgtcggac ttttagaaga
    14761 agccgccatt gatggcattc cggccatcgt cattgatccc aagggagatc tctccaatct
    14821 tctcctgacg ttcccgcaac tgcgcggcga agactttgcc ccctggatca atgaggacga
    14881 tgcccgcaag aaaggacttt cgcctgccga ctacgcgacc caacaagctg cgttgtggca
    14941 gaaaggctta ggcgaatggg gtcagagcgg cgagcggatt caaaa
//
LOCUS       DAOQDS010000084        14514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1808_length_14514_cov_18.872052, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000084 DAOQDS010000000
VERSION     DAOQDS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14514
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1808_length_14514_cov_18.872052"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /gene="coaD"
                     /locus_tag="PK589_16100"
     CDS             <1..257
                     /gene="coaD"
                     /locus_tag="PK589_16100"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Catalyzes the conversion of ATP and pantetheine
                     4'-phosphate to diphosphate and 3'-dephospho-coA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNJ21184.1"
                     /translation="TSGAHAILRGLRAISDFEHEFQMALVNRKLAETVETVFLMPSEE
                     YSYLSSTIIKDVASHGGSLKDFVHPEVARRLQERIRSFKG"
     gene            254..1447
                     /locus_tag="PK589_16105"
     CDS             254..1447
                     /locus_tag="PK589_16105"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNJ21185.1"
                     /translation="MKLAARVGRIVPSPTLSITATAKAMAAQGIDVIDFASGEPDFDT
                     PEPVKAAAEAAIRAGFTKYTPSSGIDELRAAIVDKLKDEQGLQYDKSQILVSCGAKHS
                     LYNVAEALLEAGDELIIPVPFWVSYQDQTLLNDATPVLLQTQERDGYTISQEALEAVI
                     TPRTKAIIVNSPCNPTGATYDRNTLERIAAVALRHDLVIISDEIYEKVLYDGVQHVSI
                     ASLSPEVAARTVVINGVSKAYAMTGWRIGYAAGPKPLLTAMANIQSQSTSNPCSISQK
                     AAVAALRLGNPFTTVMVAEFDRRRRLMVERLNKMPGMTCRMPTGAFYAFPNVSGLLTK
                     RWNNQPIGSAANLATFLLNEAQVALVPGEPFGSDVHIRLSYATSMEAIERGLTRIEAA
                     IRRLS"
     gene            complement(1744..1935)
                     /locus_tag="PK589_16110"
     CDS             complement(1744..1935)
                     /locus_tag="PK589_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21186.1"
                     /translation="MLELLLEAEVVDVVAAGAGAAGVDVEAGLLLVVSDEAGVFALSV
                     PVAAGLSEVSLPPGFILSE"
     gene            complement(2098..2541)
                     /locus_tag="PK589_16115"
     CDS             complement(2098..2541)
                     /locus_tag="PK589_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21187.1"
                     /translation="MLIRSRRAVIGLSMLGFSSMLLLAGCDFWPPALQAQIEQLHSEA
                     QQAAADKALLQNQLNAANKAKEELQIRVEDLTRVNKEKSAMIASLEHTIAAARERAVK
                     AAKAPAAKPAAKTTVKKPVKAPSKATSKSSAKSSTVKKPKSAQLR"
     gene            complement(2590..3237)
                     /locus_tag="PK589_16120"
     CDS             complement(2590..3237)
                     /locus_tag="PK589_16120"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNJ21188.1"
                     /translation="MALVLPFIDKTFDSILPEVERVLAAQGLIALPTETYYGLAVRPS
                     DEVAVRRLIDVKGRPADKPILVLIGGRDQLPTLVSSIPPAAAALMDLFWPGPLTIVFP
                     AATGLSPLLTAGTGTIGVRWSPLATLQRLLKRTGPLTGTSANRSSEPPLDQAASVQHT
                     LGPVLDLILDGGRTPGGSASTIVDACGEPRVLRAGVVSTERIRAALAQRGHTLSS"
     gene            complement(3259..4536)
                     /gene="purD"
                     /locus_tag="PK589_16125"
     CDS             complement(3259..4536)
                     /gene="purD"
                     /locus_tag="PK589_16125"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNJ21189.1"
                     /translation="MKILVVGSGGREHALVWKIAQSPRKPQIFCAPGNAGIEGLATCV
                     PVKADDIAGLKAFALKEGIDLTVVGPEAPLALGIADEFRKARLKIFGPTKAAARLESS
                     KSFSKDIMAASRIPTAVSKSFEQMDQALAYVDAQPVPIVVKADGLAQGKGVVVAMTRE
                     EAKQAVRDAMEKSVFGQAGQRVLIEEFLDGEELTIMAFTDGNTVVPMIPAQDHKRVGD
                     GDAGPNTGGMGAYAPAPIATAALRDQVTRQVLQPTVDAMARLGCPFQGVLYAGLMIVK
                     GTPYVLEFNARMGDPETEVVLPLLKTDLVDVMEAVVEHRLDELQVEWRQDTAVCVVMT
                     SPGYPGSYETGFPIEGLPAQSGASPIAVFHAGTKREAGQVVTAGGRVLAVAAWAPSLL
                     QARAQVYQAVPGIRFQGRHYRTDIAHRALPRQS"
     assembly_gap    4549..4648
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4649..6177)
                     /gene="purH"
                     /locus_tag="PK589_16130"
     CDS             complement(<4649..6177)
                     /gene="purH"
                     /locus_tag="PK589_16130"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNJ21190.1"
                     /translation="MASIARALISVSDKTGVVEMAKGLAALGAEILSTGGTAKALRDA
                     GVAVTDVAAYTGSPEILDGRVKTLHPKIHGGLLGRRGVPDHVKQMQQHGIGNIDVVVV
                     NLYPFESTIAKPNCPFEEAIENIDIGGPSMLRSAAKNHEDVLVVVDPSDYTRVLEAVK
                     AGSVTPALRRELAMKVFQHTARYDSLIAGYLEKQVQGSEVKFPAILSLQFERVETLRY
                     GENPHQQGAFYREMQAKEPAVSRGKILHGKAMSYNNFLDSNSALELVKEFDQTAVAII
                     KHNNPCGCALGATPVEAYVKARATDPVSAFGGVIAFNRPVDLAAAKEITSTFVEVVIA
                     PGFAEDALAELKRKKDIRLLDVGPLSKATAEGYDLKKLVGGLIVQDRDLGVIKDIKAL
                     AVPTSRKPTEQEYAACAFAWVVCKHVKSNAIIYGRPGEIVGIGAGQMSRVDSVKLAAM
                     KAQSSVKGCVMASDAFFPFRDGIDAAAEAGITCVIQPGGSIRDPEVTKAVDEHGMAMI
                     LT"
     gene            6522..7433
                     /locus_tag="PK589_16135"
     CDS             6522..7433
                     /locus_tag="PK589_16135"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ21191.1"
                     /translation="MSRGIIFGVGLFALTLLALICIPRHLPVTSSATGHPSFSAHIEN
                     GHLTLSGAMGSEEAKLAAVARAQELAKNLKLRVTDNLDVLEDGGAAAWEAAVPALLTQ
                     VAALHQHQASLTVSRQTATIKGAASNADAKTKLVHEMSSLLGTAMHVQDQVIVTSAGS
                     ASPSSQPTPTLTATPPTAHASRAQVQASLDEIVRGEHIAFESNSAVLTPKGRAVIDKL
                     IPALKRSPESAIEIGGHTDSYGDPDYNLQLSRTRAESVRQYLADHGVTNRLTAVGYGA
                     SRPLSQDRTRAASKKNRRIEFRVKEER"
     gene            7438..8685
                     /locus_tag="PK589_16140"
     CDS             7438..8685
                     /locus_tag="PK589_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21192.1"
                     /translation="MGALILQMIGCLLVAATIGLMMGWLLRSFATSEKRQQLSEIATR
                     LRGREHELDTLNHELKVRTSAVQMLEGKMITSEAALKDLTADLATKVEQLSALQTELR
                     EKGVHLHAAERERDTLRREVEAAENKFKAQQAGFAEMQSQMENAEDVLVSRDQEIATL
                     KTWVEQLAPKDAEIARLRARTEELEPFQERSRRLERELEQDRARATATIQGKDQELAR
                     HQAHIVEMEAELTRLRTQNQEDLTRFTETLQQRDQDIQRLRATVEELEVFRGEVEKKE
                     IQLREAEERRVMDVSEREEEIGALRKRLVEYRVAQRHQAQAKSNNQAEPASHNSSSSR
                     SAGKSRSAQKDDLKQIHGIGPVMERVLNRMGMFTFRQIAEWKDQDVEHMASELNTFPD
                     RIRHDNWIAGAKEQHFLKYGEEL"
     gene            8776..9561
                     /locus_tag="PK589_16145"
     CDS             8776..9561
                     /locus_tag="PK589_16145"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNJ21193.1"
                     /translation="MITSTFCMLPGVGLRTERQFWGQGAWTWDGFLSTRDLRGLSLAR
                     KALFDDRITEAQERFARQDSRYFAKTLAARHQWRLYEWLRERALYLDIEIDSFGQITV
                     VGLYGRGRYTALVRNESLSLLRLKEELSQYDLLVTFCGSTFDLPMLRAHFSDLPLDQP
                     HIDLCGIGRQLGYRGGLKAIERRMGITRLPAIQGFSGDDAVRQWNRWRHQRDEQARDL
                     LLAYNEADCVNLQPLADAFYCRLAVASGLEEIRLQGRTDAVSE"
     gene            complement(9605..10753)
                     /locus_tag="PK589_16150"
     CDS             complement(9605..10753)
                     /locus_tag="PK589_16150"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNJ21194.1"
                     /translation="MTALFSYGFRPFFLGAAWFAGLAVPAWIVMLSLGIGSTGLAAPR
                     EWHVHEMLFGFLPAVITGFVLTAVPNWTDRPAIQGRELAGLFCLWLAGRVLLAWPWVP
                     PVFAAIVDAAYLLVLAGFLWREISGGKSWSHAPIAVLLSLYAGANLLFHLFALNGTAT
                     DLPERMALGLDLTLLAFIGGRVTPNFTREYLVHARRPEKPARFTHLDMVSIGAVAFAS
                     TFWALLSQDAVAGWFLILAGVLNLVRLSRWYGWFTWREPLVFVLHWGYGWLILALVLL
                     GCAALGVGLPKEDAVHALTTGAVGVMTLGIMTRASLGHTGRQRHADAATIAMYALVTC
                     GAILRVFVAGTGLPTGLVLGAAATCWSGAYLLFALVYGPYLLRPSLDE"
     gene            complement(10931..11923)
                     /locus_tag="PK589_16155"
     CDS             complement(10931..11923)
                     /locus_tag="PK589_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21195.1"
                     /translation="MFHEFCFGFWAFLRNAAGSLYHIVIVALSAGIALLLPAAARQFM
                     SFWPRVEHDTWSVIAIEMAAAVLSMAGLNYIHRSIRDRGLADAALGAGLVSFFPRRGP
                     AARERIQALKDEQGTGRSVMVLGSSGAGTLVDQVGDLSSVLDKCLEAKILLVNPFNQG
                     ARARIQALAHPSCTYPAFREEVRQSIALLKRLKAIGKVVKLKLYADAPLIKLVVFGDY
                     LWLQHYHAELDVQNMPEYVLQRNHQQHGLYTLYAHYFMQRWESSELPEYDLDTDELVY
                     RSRTGQEIRREPVEIGTRHVAAHPTDTAGVDTREAFQLLHAPGPYLPVRGYDVE"
     gene            complement(12169..13011)
                     /locus_tag="PK589_16160"
     CDS             complement(12169..13011)
                     /locus_tag="PK589_16160"
                     /inference="COORDINATES: protein motif:HMM:NF016100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21196.1"
                     /translation="MHRKSPRSGKRSAAPALAKAVCTESQALEQAQVLISKAWEATTA
                     RQAASLARQALEICSDCVDAYTVLAESEARSAEEACQWYEQAVDVGRRHLGETLFAQQ
                     AGHLGRLPETRPYMRARQGLADCLWALGRREESLAHGEALLELNPDDDQGIRQSLLSR
                     YLAVGNEAGAERLLHRYAHDSSAAFLWSRVLLDLRREDQAAAKDDLRAAMQGNPHVAD
                     LFSGKRTPPARLPERFTPGDRHEAALYLAGFAEAWLASSDAMEWLMEQLTMRVSRQRS
                     PQAR"
     gene            complement(13066..>14514)
                     /locus_tag="PK589_16165"
     CDS             complement(13066..>14514)
                     /locus_tag="PK589_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21197.1"
                     /translation="TALFYSVLRAVGHLQVVDGTQELQRPGRQSFSADVQPRLYEVGR
                     HGAVDLSPDPVHQSSAPSSIHPPRPDVDPVSGALRLPSWRTILLLLTAVGLMFWCAIL
                     INSELWAMWRRSRLSFFAGPLLVVLLAAGLLCSAMLDGTKGEPFSFTDGISVWPTTSL
                     RLLAFMLCVIFFAHSWWRLWESDRTLIRRFRLRRLDALLSRRFRSWIGIHRWLPPLLG
                     HAEARELWHEYRLLGAWRERLRRVVPQVVVYGCFGFVLLLQFGFPVTPCRGDACFTLN
                     SLILGVSVVAMTLLMFYVVDATRLCRRLILIMIGTRIEWPERLLAREAAKLGVEQDYL
                     HEWLGIQFIAKRTAVISAMIYYPFVIVFLMAVARHSYFDRWDFPAGLLVIFTLNALYA
                     FGNGVFLRRSAEEAKREAVRQLQHRLKSLSDEPVSKQTKRRHIERMVELIEQTRDGAF
                     LPFTQHPLFGAIALPTGGTGLVFLLDYLATLF"
BASE COUNT         3199 a         4530 c         4078 g         2607 t
ORIGIN      
        1 gcaccagcgg cgcccatgct attctgcggg gactgcgcgc gatttctgat ttcgagcacg
       61 agtttcaaat ggcactggtc aatcgaaaac tggcggaaac cgtggagacg gtcttcctca
      121 tgcccagtga agaatattcc tatctctctt ccaccatcat caaggatgtc gccagccacg
      181 gcgggtcact gaaagacttt gtgcacccgg aagtggcacg gaggctccaa gaacgaattc
      241 ggagtttcaa aggatgaaac tggcagcccg tgtcggacga attgtgccct ctccaaccct
      301 gagcattacc gccaccgcaa aagccatggc ggctcaaggt atcgacgtca tcgattttgc
      361 ctccggcgaa ccggactttg atacgccgga accggtgaaa gccgcggccg aggccgccat
      421 tcgagcaggc tttacgaagt atacgccgtc gtcagggatc gacgaactgc gtgccgcgat
      481 tgtcgataag ttaaaagacg aacagggact tcagtatgac aagtcccaga ttctggtgtc
      541 ctgcggcgcc aaacattctc tctataacgt ggcggaagcc ttgttggaag cgggcgatga
      601 actgattatc ccggtcccct tctgggtgtc ctaccaggat caaaccctgc tgaacgatgc
      661 caccccggtc ctgcttcaga cacaggagcg ggatgggtac acgatttcgc aagaagccct
      721 ggaagccgtc atcaccccgc gcaccaaagc gattatcgtc aacagcccgt gtaatcctac
      781 cggcgcgacg tacgaccgga acactctgga acgcatcgcc gccgtcgctt tgcgtcatga
      841 cctcgtcatc atttcagacg agatttacga gaaggtgttg tacgacggcg tgcagcacgt
      901 cagcatcgcc agcctgagcc cggaagtcgc agcacgcaca gtggtcatca acggtgtttc
      961 gaaagcctat gcgatgaccg gttggcgcat cggctatgcc gcgggtccga agccgttgct
     1021 gacagcgatg gcgaacatcc aaagtcaaag cacctcgaac ccctgctcca tttcgcaaaa
     1081 agccgccgtg gcggcgctcc gtttgggaaa cccctttacc actgtgatgg tggcggaatt
     1141 cgatcgtcgg cgacgcctga tggtcgagcg cctgaacaaa atgcctggta tgacctgtcg
     1201 catgccgacc ggagcatttt atgcgttccc caatgtgagc ggcctgctga ccaagcgatg
     1261 gaacaatcaa cccatcggct cggccgcgaa cctggcgacc tttttgctca acgaagcgca
     1321 ggtggcgttg gtgcctggtg aaccgtttgg aagcgacgtc catatccggc tctcctatgc
     1381 gacctcgatg gaagcgatcg aacgcgggtt gacccgcatc gaagccgcca ttcgccggct
     1441 gtcgtgatca acaggatctc gacacctcgg ccccttgact gctctcgaca cgatgttatc
     1501 aggggagagg tcttgcagct gacggatcga gccaatcaag accgagtgac gccctaggat
     1561 cagaggagga agacccgtgc caatctatga gtatcaatgt tccagctgtg cgcatcgctt
     1621 cgaggtgaag cagagcatca aggatgaccc catcaaggaa tgcgtacgct gcggcaaaga
     1681 agtgacgaag ctgatttcgc cccctgccat tatgtttaaa ggcagcggtt ggtatatcac
     1741 cgactactcg gacaaaatga aaccgggggg cagcgatacc tccgacaagc cggcagctac
     1801 cggcacagac aaggcgaaaa ccccggcctc gtcggatacc acaagcagca gccccgcctc
     1861 cacgtcgacg cctgcagcgc ctgcgccggc cgcgacgaca tccactactt cggcttcgag
     1921 cagcagctcg agcacatcca gccccagtgc agcggggtct cagtcgtcag ccccatcctc
     1981 cagtaaatcg tgagaaggct cttgccttca cgcgatgaga gtggtcgatc ggctcgatct
     2041 ttctgacgtg ggaccaaccg aacctgctgg ccgggctgta cgctgacttg agcaggctta
     2101 tcgaagctgg gcagatttcg gttttttgac ggtcgaggat ttggcagagg atttcgacgt
     2161 cgctttcgat ggcgctttga ccggcttctt caccgtcgtt tttgcggcag gcttggccgc
     2221 aggagccttg gcggccttca cagcccgctc cctcgctgcg gcaatcgtgt gctccaaact
     2281 cgcaatcatc gccgactttt ctttattcac ccgcgtgaga tcttcgactc gaatttgcag
     2341 ctcttctttg gccttattcg ccgcgttgag ctgattctgc aagagtgcct tatccgccgc
     2401 agcctgttgc gcttccgaat gtaattgttc gatttgggcc tggagcgccg gcggccaaaa
     2461 gtcacatcct gccagcagca gcatactgct gaacccgagc atcgacagtc ctatcaccgc
     2521 gcgacgtgaa cgaatgagca taagtggtcc tcccgtgtgg gtgaattata cccacaacgc
     2581 accatcaaat catgatgaaa gtgtgtgtcc ccgttgagcg agggccgcac ggatccgctc
     2641 cgtcgacacc acccccgctc gcagcacgcg cggttcgcca caggcatcca cgatggtgga
     2701 cgctgatcca cccggcgtgc gtccgccgtc cagaatcaga tcgagaaccg gcccgagagt
     2761 gtgctgcaca gacgcggcct gatcgagagg cggttcagat gaccgattgg cgctggtccc
     2821 tgtcagcgga ccagtgcgct tgagcagacg ctgcagcgtg gccagcggtg accatcgaac
     2881 gccgatcgtg ccggttcccg ccgtcaacaa cggtgacaag ccggtcgcag caggaaatac
     2941 gatcgtcaag ggacccggcc aaaacagatc catcagcgca gcggcggccg gaggaattga
     3001 ggacaccaac gtcggtagct gatcgcggcc accgatgagt acgagaatcg gcttgtcggc
     3061 cggccggcct ttgacgtcga tcaggcggcg caccgccacc tcgtcgctgg ggcgaaccgc
     3121 caatccataa taggtttcag tcggcagtgc gatcagcccc tgcgcggcca ggacacgttc
     3181 gacctcaggg aggatgctgt cgaaggtctt gtcgatgaag ggaaggacca gagccatgtc
     3241 aggcctgtcg agcgcggttc aagactggcg tggaagggcg cggtgggcga tgtctgtgcg
     3301 atagtggcgt ccctggaagc gaatacccgg cacagcctga tagacctgtg cccgcgcttg
     3361 cagtaaagac ggagcccagg ccgcgactgc caacacgcgc cccccggccg ttaccacctg
     3421 ccctgcttcc cgtttggtcc cggcgtgaaa taccgcaatg ggcgacgccc cagactgcgc
     3481 cggcaaacct tcaataggga acccggtctc ataggaaccg ggataccccg gtgaggtcat
     3541 caccacacag acggcggtgt cctgccgcca ttcaacctgc agctcgtcga gacgatgttc
     3601 aaccaccgcc tccatcacgt cgaccagatc ggtcttcagc aagggcaaca cgacttccgt
     3661 ttccggatct cccatacgtg cattgaattc cagcacgtag ggagtcccct tcacgatcat
     3721 gaggcccgcg tacagcacgc cttggaaagg acagcccaaa cgggccatcg catccacggt
     3781 cggttgcagt acctgccgcg tcacctggtc gcgcagcgca gccgtcgcaa tgggagccgg
     3841 agcgtaggcg cccatgccac cggtattggg tccggcatca ccgtcgccca cccgcttgtg
     3901 atcctgcgcg gggatcatcg gcacgacagt gtttccatcc gtaaatgcca tgatggtcag
     3961 ctcctcgcca tcgagaaact cttcaatcaa cacccgttgg ccggcctggc cgaagacgga
     4021 tttctccatg gcgtcgcgca ccgcctgttt ggcttcctcg cgcgtcatcg ccaccactac
     4081 gcccttgccc tgcgcgaggc cgtcggcctt caccacaatc ggcacgggtt gagcatccac
     4141 atacgccaac gcctgatcca tctgctcgaa gcttttcgag accgccgtgg gaatccggct
     4201 ggccgccatg atgtccttcg agaagctttt gctggattcc agccgtgccg cagccttggt
     4261 gggaccgaag atcttcaaac gtgctttcct gaactcatcg gcaatgccca gcgccagcgg
     4321 cgcttcagga ccaacgacgg tcagatcaat gccctccttg agagcaaagg ccttcagccc
     4381 ggcaatatca tctgccttga ccggcacaca ggtcgccagg ccttcgatgc cggcattgcc
     4441 cggcgcgcaa aagatctgcg gcttccgcgg actctgggca attttccaga ccagggcatg
     4501 ttcccgacca ccactgccga cgaccagaat tttcacagct caacctttnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnngt gagtatcatc gccatcccgt gttcatctac
     4681 ggccttggtc acctcggggt cacgaataga cccgccgggc tgaatcacac aggtgatacc
     4741 ggcttctgcc gcggcatcga tgccgtcccg gaacgggaag aacgcgtcgg acgccatgac
     4801 acagcccttc acggacgact gcgctttcat cgccgccaac ttcaccgaat ccacccggct
     4861 catctgtccg gcgccgatgc cgacgatttc accaggccga ccgtaaatga tggcattcga
     4921 cttcacatgt ttacagacca cccaggcaaa tgcacaggcc gcatattcct gctcggtggg
     4981 cttgcgtgag gtcggcacag cgagtgcttt gatatccttg atcacgccga gatcccgatc
     5041 ctggacgatc aatccaccga ccagcttctt gagatcatat ccttccgcgg tcgccttgct
     5101 gagcgggccg acatccagca gacgaatgtc cttcttccgc ttgagttccg ccagcgcatc
     5161 ctcggcaaaa cccggcgcga tgacgacttc cacaaacgtc gacgtaatct ccttcgccgc
     5221 tgccagatcg accggccgat taaacgcaat caccccgccg aacgccgaca caggatccgt
     5281 cgcgcgcgct ttgacgtagg cttccacggg cgtggcgccg agggcgcagc cgcagggatt
     5341 gttgtgcttg ataattgcta cggcggtctg gtcaaactcc ttcacgagtt cgagcgccga
     5401 attggagtcg agaaaattat tgtacgacat ggctttgccg tgcaggatct tgccgcgtga
     5461 cacggccggc tccttggcct gcatctcacg atagaaggcc ccctgctggt gcggattttc
     5521 accgtagcgc agcgtctcca cccgctcgaa ctgcaaggag agaatggcgg ggaacttcac
     5581 ctcgctccct tgcacctgct tttcaagata cccggcgatc agactgtcat agcgcgccgt
     5641 gtgctgaaag accttcatcg ccagttcgcg gcgcaatgcc ggcgtgacgg aaccagcctt
     5701 cacggcctcc aatacgcggg tgtaatcaga cgggtcgacc acgacgagca catcctcatg
     5761 gttttttgcg gcggagcgca acatggaggg accgccgatg tcgatgttct cgatcgcctc
     5821 ctcaaacgga caattgggct tggcgattgt cgattcgaag ggatacagat tcaccacgac
     5881 cacatcgatg ttgccgatgc cgtgctgctg catctgcttg acgtggtcgg gaacgccccg
     5941 acgccccagc aaaccgccgt gaattttcgg atgcagcgtt ttcacgcggc cgtcgagaat
     6001 ctccggcgac cccgtatagg cagccacatc cgtcaccgcc acacccgcat cacgcaaggc
     6061 tttggcggtt ccgcccgtgg acaatatttc cgcgccaagg gctgccaacc ctttggccat
     6121 ctcaacaact ccggtcttgt cagaaacact gatcagtgcc cgcgcaatgc tggccatgat
     6181 gaactccttc aactatggat atcgtgtggg tgggatcaca ttggacgaaa aaacgccgtg
     6241 acaatagcag atgcctcgac gccactgcaa gccgatcgag tatgccatca ccgcatcagc
     6301 gctgtggcag aatccgatga gggaatcagg aggacgtccg ctacccagaa tccaggctag
     6361 catgagtccc agcgatgtga tacccttcgc aattcgtaat cgagcagccg gcgctgccta
     6421 aacaggaaat caagtaacac tcgaagtctt caaccactcc acatcctgtg gcgttcgacg
     6481 ccgcagcgag gcctcgtccc cgaggcgata agggctggcg catgtcgcgc ggaattattt
     6541 tcggcgtagg actctttgca ctcacattat tggcgctcat ttgtatccct cgacacctgc
     6601 cggtgacgtc gtcggccacg gggcatccct cgttcagtgc ccatatcgag aacggtcacc
     6661 tgacactttc cggggcaatg ggaagcgaag aggccaaact cgccgccgtc gcacgtgcgc
     6721 aggaactcgc caagaacctc aagttgcgtg tcaccgacaa tctcgacgtg ctggaggacg
     6781 gcggagcggc agcatgggaa gccgcagtgc ctgccttgct cacgcaagtg gcggccctgc
     6841 accaacacca ggccagcctc actgtctcgc gccaaacagc gaccatcaag ggcgcggcct
     6901 cgaacgcgga cgccaaaacg aaactcgtgc atgagatgag ctcgctgctc ggcaccgcga
     6961 tgcatgtgca ggaccaggta atcgtcacct cggccggctc cgcctccccg tcatctcaac
     7021 cgacgccgac actgaccgcc acccctccga cggcgcacgc ctctcgcgcg caagtgcagg
     7081 ccagcttgga tgagatcgtg cgcggagaac atatcgcgtt tgagagcaac agcgccgtac
     7141 tcacccctaa aggacgagcg gtgatcgaca agctcattcc cgcgctcaag cgctcgcccg
     7201 aatcggccat tgaaatcggc ggccataccg attcctatgg cgacccggac tacaatctcc
     7261 agttgagccg gacgcgagcc gagtcggtcc gccaatatct ggccgatcat ggagtgacta
     7321 atcgattgac cgccgtcggc tatggcgcct cccgtcccct gagccaggac cggacgcgcg
     7381 ccgcctccaa aaagaaccgg cggattgaat ttcgtgtgaa agaggaacgg tgaacgtatg
     7441 ggagccttaa ttctgcaaat gatcggatgc ttactggtgg cggcgaccat cgggctcatg
     7501 atgggctggc tgttgcggag tttcgccacg tcggagaaac gccagcagtt atccgaaatt
     7561 gccacccggc tccgcggtcg cgaacacgaa ttagacaccc tcaaccatga gttgaaagtg
     7621 cgcacctccg ccgtccagat gctggaaggc aagatgatca cctccgaagc ggcgctcaaa
     7681 gatttgaccg ccgatctggc cacaaaggtc gaacaactct cggcgctgca gaccgaactc
     7741 cgtgaaaaag gggtgcacct ccatgcagcc gagcgcgaac gggatacgct tcgccgggaa
     7801 gtcgaggccg ccgaaaacaa gttcaaggcg caacaagccg gcttcgccga aatgcaatcg
     7861 cagatggaga atgccgaaga cgtgttggtc agccgggatc aggaaatcgc cacactaaaa
     7921 acctgggtgg agcaacttgc gcccaaggat gccgaaattg cccgcctgcg ggcgcgcacc
     7981 gaagaactgg aaccgttcca ggaacgaagc cgtcgattgg agcgggaact cgaacaagat
     8041 cgcgcgcggg ctacagctac cattcagggc aaggatcagg agctcgcccg tcaccaggca
     8101 cacatcgttg agatggaagc agaactcact cgcctgcgga cacaaaacca ggaagacctc
     8161 acgcgcttca ccgaaacgct gcagcagcgg gatcaggata ttcaacgcct ccgcgccacc
     8221 gtcgaagaat tggaggtgtt ccgtggagag gtggagaaaa aggagattca actccgcgag
     8281 gcggaagaac gccgggtgat ggacgtcagt gaacgggaag aagaaatcgg cgcgctacgc
     8341 aagcggctgg tcgaataccg cgtcgcccaa cggcaccagg cgcaggcgaa atcgaacaac
     8401 caggctgagc cggcctcgca caactcctcc agctcccgga gtgccggaaa atcacggtct
     8461 gcccagaaag acgatctcaa gcagattcat gggattggac cggtgatgga gcgcgtgtta
     8521 aatcgcatgg gcatgtttac atttcgccag atcgccgaat ggaaggatca agacgtcgaa
     8581 catatggcgt cggagctcaa caccttcccg gatcgcatcc gccacgacaa ttggatcgcg
     8641 ggcgccaagg aacaacattt tctgaaatac ggtgaggaac tgtagtccgt cctgatcaga
     8701 tcgtccgttc ccctcctcgc tgttattcca gccccttcct gctgcacgct gtaccgaccc
     8761 gttcggagac cctacatgat tacctccacc ttctgcatgc tccccggagt cggcctgcgc
     8821 acggaacgac agttctgggg ccaaggcgcc tggacgtggg atggctttct gtcgactcgc
     8881 gacctacgcg ggctcagcct cgccagaaaa gccctcttcg acgaccggat cactgaggcg
     8941 caagagcgat tcgcacgaca ggatagccgc tactttgcga aaaccctggc ggctcgccat
     9001 cagtggcggt tgtatgaatg gttacgggaa cgggcgctct atctcgatat cgaaatcgat
     9061 tcgttcggac agatcacggt ggtcgggttg tatggccggg gacgctatac cgcgctcgtc
     9121 agaaacgagt cgctgagcct gcttcgcctg aaagaggaat tgtcacaata tgatctcctc
     9181 gtgacattct gcggatcgac gtttgatctt ccgatgttgc gggcgcactt ttcagacctg
     9241 cccctggacc agccgcacat cgatctctgc ggcatcggcc ggcaattggg gtatcgcggg
     9301 gggctcaaag ccatcgaacg ccggatgggc atcacccgcc tgccggcgat ccaaggcttt
     9361 tccggagatg atgccgtccg ccaatggaac cggtggcggc accaacgcga tgaacaagca
     9421 agggatctct tgctcgcgta caacgaagct gactgcgtga acttacagcc tctcgccgat
     9481 gccttttatt gccgtctggc cgtggcgtcc gggctcgagg agattcggct tcagggcagg
     9541 acggatgctg taagcgagta gaacatcctt cctgagggtg attgccttac tcaacctgcc
     9601 ttcactactc atccagactc ggacgcaaga ggtacggccc atagaccaac gcgaacagca
     9661 gatacgcgcc gctccaacag gttgcggccg caccaagcac gagaccggta ggcagaccgg
     9721 taccagccac gaacactcgc agaatcgctc cgcaggtgac cagggcatac atggcgatcg
     9781 tcgcagcatc ggcatgccgc tggcgtccgg tatgtcccaa gctggcgcgg gtcatgatgc
     9841 ccaaggtcat gacacccact gcccctgtgg tgagcgcatg caccgcatcc tccttcggca
     9901 ggccgacgcc tagcgcggca catccgagca ggacgagcgc cagaatgagc cacccatatc
     9961 cccaatgcag cacaaacacg agcggctccc gccatgtgaa ccagccatac caccgcgaca
    10021 accggacgag attaaggacg ccggcgagga tcagaaacca gccggccacg gcgtcctggg
    10081 acaacaacgc ccagaatgtt gaggcgaacg caactgcccc gatcgacacc atatcgagat
    10141 gagtaaaccg ggcgggtttc tccggccggc gagcgtgcac cagatactca cgggtaaaat
    10201 tcggcgtcac gcgcccgccg atgaaggcca gcaaggtcag atccagcccc aacgccatgc
    10261 gttcgggcag atcagtcgcg gtcccattga gcgcgaacag atgaaaaagc agatttgcgc
    10321 ccgcatagag actgagcagg acggcgatcg gcgcatgact ccagcttttt cctcctgaaa
    10381 tttcgcgcca aaggaatcct gccaacacca gcagataggc cgcatcgacg atggctgcaa
    10441 agaccggggg tacccagggc catgccagca gcacacggcc cgcgagccac aggcagaaca
    10501 ggcccgccaa ttcccgtccc tggatggccg gacgatctgt ccagttcggc accgccgtca
    10561 gcacaaaccc ggtgatgacc gccggcaaaa atccgaacaa catttcgtgc acatgccatt
    10621 cgcgaggtgc cgccaggccg gttgaaccaa tcccgagcga caacatcacg atccaggcag
    10681 gaacagccag gcccgcgaac caggcggcgc cgagaaaaaa cggacggaag ccgtaggaaa
    10741 ataatgcggt cattgacgac actccgcttc ctttcgtctt acgacttatc atccttcacc
    10801 ctctcgtgga tacgaccggc cggcgaggca ttgccatggc cgccctagtc ataccacagc
    10861 gccgaggagt gagccatgac aagggagagc agcgagcacg cgatgaacgg acggatgaat
    10921 gcggcgactc ctattcgacg tcgtatccgc gaaccggcag gtacgggccc ggggcgtgca
    10981 acaattgaaa cgcttcacgc gtatcgactc ccgccgtatc cgtgggatga gcggcaacgt
    11041 gtctggtccc gatttccacc ggctcccgcc ggatttcttg accggtccgg ctccgatata
    11101 ccagctcatc ggtatccaga tcgtattcag gcaactcgga gctctcccac cgctgcatga
    11161 agtagtgggc gtacaacgta tacaagccgt gctgctggtg gttccgctgc aggacatatt
    11221 ctggcatgtt ctgaacatcc agctccgcat ggtaatgctg caaccagagg tagtcgccga
    11281 agaccaccaa cttgatcaac ggagcatcgg cgtagagctt cagcttcacc accttcccga
    11341 ttgccttgag acgcttgagt agcgcgatac tctgccggac ctcctcgcga aaggccggat
    11401 aggtacagga agggtgagcc aacgcctgga tccgcgcccg tgcgccctga ttaaatggat
    11461 tcaccaggag aatcttcgcc tctaggcatt tgtcgaggac cgacgacaga tcacccactt
    11521 ggtccaccag agtccccgcg ccgctggatc cgagcaccat cacggaacgg ccggtgccct
    11581 gctcgtcttt taaggcctga atgcgctctc gcgctgccgg cccgcgccgg ggaaagaatg
    11641 agacgagacc ggcaccaagt gcggcatcgg caagtcctcg atcccgtata ctccgatgga
    11701 tatagttcaa gccggccatc gacagtacgg cggcagccat ttcaatcgca atcacagacc
    11761 aggtgtcatg ttccacccgc ggccagaacg acatgaactg cctggcggcg gccgggagga
    11821 gaagcgcaat gcctgcgctt aaggcaacaa tgacaatgtg gtacaggctc cctgccgcat
    11881 tgcgcaggaa tgcccagaaa ccaaaacaga attcatgaaa caacgaaaca cctctagacg
    11941 ggtaaaaaag atccccacgg cggtgacgag ctggcagtgc gaacgacgga ggaacaacct
    12001 tcggcggtag gctcagcgga aacggcacgt ctgcgggtac aaacaacaat cgcgtaagag
    12061 ctcagagatc caagggctgc tgcccccggc tgcagaaggt cttacgcgaa caactgcact
    12121 gtaacaggtc tgttgtcgcg acgcaaggac ggcaggcttg cgagaagttc aacgggcctg
    12181 aggagaccgt tgtcgtgaga ctcgcatcgt caactgctcc atcagccatt ccatcgcatc
    12241 tgacgacgcg agccaggcct cggcaaaccc cgcaagatag agagccgcct cgtgccggtc
    12301 acccggcgtg aaccgttctg gcaaccgggc cggcggtgtc cgtttcccgc tgaacaagtc
    12361 ggccacatgg ggattgcctt gcatcgccgc tcgcaagtcg tcctttgccg cggcctgatc
    12421 ttcgcgtcga aggtcaagca ggacgcggct ccagagaaat gctgcggatg aatcatgcgc
    12481 atatcgatgc aagagacgtt ctgctcctgc ctcattcccc accgccagat accggctcag
    12541 caagctctgt cgaatcccct ggtcatcgtc gggattcagt tccaagaggg cttcgccatg
    12601 ggcgagggat tcctccctcc ggccgagcgc ccagagacag tcggccaagc cctgacgcgc
    12661 acgcatgtag ggcctggttt cgggcagtct ccccaaatgg cccgcctgct gcgcaaacaa
    12721 tgtctcgccg agatgacgtc gtccgacatc tacggcttgc tcataccact gacaggcttc
    12781 ctctgccgaa cgcgcttcgg actctgccaa tactgtgtag gcatccacac aatcggaaca
    12841 gatctccaat gcctgacggg ccaacgacgc cgcctgtcgt gcggttgtgg cctcccacgc
    12901 tttagagatc aagacctgcg cctgttccaa tgcttggctt tctgtacaga ccgccttcgc
    12961 gagcgccggc gcggcagatc gtttgccgga gcggggagat tttctatgca tgaatacggg
    13021 gctccttcca gcggccctgg cgggcgatcc gccaacagct ctccgtcaga acagcgtggc
    13081 caggtaatcc agcaggaaca ccaggcctgt gccgccggtc ggtaatgcga tcgcgccgaa
    13141 caatggatgc tgcgtgaagg gcagaaatgc gccatccctg gtctgttcaa tcagctcgac
    13201 catgcgttcg atgtggcggc gtttggtctg tttactgacg ggctcgtcgg acaagctttt
    13261 gagccggtgt tgcaattgcc gaaccgcttc gcgcttcgct tcctcggctg atcgccgcag
    13321 aaacacacca ttgccgaacg catagagcgc gttcagggta aaaatgacaa gcaagcccgc
    13381 cggaaagtcc cagcggtcga aataactgtg ccgcgccacc gccatcagga acacgatcac
    13441 aaacggatag tagatcatgg cactgatgac cgccgtgcgc tttgcaatga actgaatgcc
    13501 gagccattcg tgtaggtagt cctgctccac gccaagcttg gcggcttccc gcgcgagcag
    13561 ccgttccggc cactcgatcc tcgtgccgat catgatcaga atcagacgac gacagagtcg
    13621 agtcgcatcc acgacataaa acatcagcaa ggtcatggcg acgacgctta cccccaggat
    13681 gagtgaattg agcgtgaaac aggcatcgcc ccgacagggc gtgacgggga aaccgaactg
    13741 caacagcaag acgaacccga agcatccata caccacaacc tgcggcacca cgcgccgtag
    13801 tcgttctctc cacgccccta acagacgata ctcgtgccaa agctcccgtg cctccgcatg
    13861 accaaggaga ggaggcaacc aacggtggat gccgatccac gaacggaacc ggcgcgatag
    13921 cagcgcatca agccgtcgca atcgaaatct ccgaatcaat gtccgatcgc tttcccacaa
    13981 ccgccaccag gaatgggcga agaagatcac gcagagcata aacgcgagca agcgcaaact
    14041 cgtcgtcggc cagacactga ttccgtcggt aaacgaaaac ggttccccct tcgtgccgtc
    14101 cagcatcgca ctgcagagca gcccggctgc aagcaggacc accagcagcg ggccggcaaa
    14161 gaaactcaac ctggagcgtc gccacatcgc ccagagttcg ctgttgatca agatcgcaca
    14221 ccagaacatc aggccgacgg cagtcaggag caacagaatc gtgcgccacg agggaagccg
    14281 cagtgccccg ctgacaggat cgacatcggg acgcggcggg tgaatgctgg acggtgcact
    14341 ggactgatgc accggatcag gactgagatc gacagcgcca tgtcggccga cttcatagag
    14401 gcgcggctgc acgtcagccg aaaaggattg ccgcccaggc cgttgaagct cctgtgttcc
    14461 atccaccact tggagatggc ccacagcccg cagcaccgaa taaaagaggg cggt
//
LOCUS       DAOQDS010000085        14457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1820_length_14457_cov_20.910776, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000085 DAOQDS010000000
VERSION     DAOQDS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14457
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1820_length_14457_cov_20.910776"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..505
                     /locus_tag="PK589_16170"
     CDS             179..505
                     /locus_tag="PK589_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNJ21198.1"
                     /translation="MGSKIYVGGLPYSATEQQLSDLFAVHGAVESARIITDKFTGQSR
                     GFGFVEMASSDEAQKAISALNGTDMGGRTLTVNEARPQEPRSGGPGRSGGGGGFNDRG
                     GKRDRW"
     gene            complement(720..1439)
                     /locus_tag="PK589_16175"
     CDS             complement(720..1439)
                     /locus_tag="PK589_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21199.1"
                     /translation="MRPHHTMAQFVRLVIAAGSLMTMICVGALWPDRVPGAEEAESDP
                     QHQRHWTFDSSSPGMLPGSYVIGTLFDGRPAGEWKILITDRAKSASQVLAQLQAKGTD
                     QTHKLLLFEGTESANLDVEVSYLAVAGKADFGGGLLWRAKDDRNYYLLRASLVEQKIR
                     VYRVVNGVQQIVKQVDRPLPATGWHKLRIVQRGCEIKALFDDAVLFRVCDTTYTSGRI
                     GLWTKADAVTYFDDLTLKVLD"
     gene            complement(1578..2474)
                     /locus_tag="PK589_16180"
     CDS             complement(1578..2474)
                     /locus_tag="PK589_16180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ21200.1"
                     /translation="MRDGVRGQRLARPLYPASIILALGLVLPFSTQASSASQETSAAK
                     SGIAGTNLLEEEQILSTTDKITQPIDTDAEAMRHNDLGVAFVFKGDVGQAIDEFTHAL
                     RLQPNYFAAHLNLANTLLDIGRHDAAMIEFKEALRLKPDDPKTHNDLGVALKEMGDPA
                     GAIAEFKTVLRRNPTDVNAHNNLGVALKAMGDLDGAIAEYRTAASLQPNDVNAHFNLG
                     LGLMEKHQPDAAIAEFRTALHLRPNDAKIRFNLAHALAGLGQRMEAAQELRQYLRLEL
                     DTPANRRWLEQAEAKLRELEMP"
     gene            complement(2665..4182)
                     /locus_tag="PK589_16185"
     CDS             complement(2665..4182)
                     /locus_tag="PK589_16185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNJ21201.1"
                     /translation="MNRWILASRAATAVLMTLTVSLTGCLQGKAYTRPSVDTPADWTH
                     VASGPLVSGTSPKTPDADWWKAFRNEELTQFVERALARNHDVRRAVSRVIEGRASVTT
                     AGAGLYPQVNVQSSYTNISISKNTLAGLGLATGQQPGPQVFARPGSSFDLWNGAADLR
                     WELDVWGRIRRGMEAASADAQAIEQDARAIALTLIGDVGQAYFRIRELDEQIEIAQRA
                     LALRRDSLEIITKRASVGLASDLDVKRTEVLVAESAGQIPDLVRLRTVEVHRLEVLTG
                     SAPGGLTLASMPLRKVVVQPDIPVGLPAQLLERRPDILQAEATLIAANARIGQARAYF
                     FPTFSITGQGGLQSAEFANWFTGNSANFSIGPSVTLPIFLGGTNVARLDAAESRYQQM
                     LEGYQQTILLAFREVADLLVSIQARAEQLTRQREQAVAATAAVGLAEVRYRKGLVNYL
                     DVLDAQRTMLAAETQLAQTERARLTDMVSLYKALGGGWSQPPGITSVPASSSMTR"
     gene            complement(4179..5171)
                     /locus_tag="PK589_16190"
     CDS             complement(4179..5171)
                     /locus_tag="PK589_16190"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ21202.1"
                     /translation="MTILNRVSVWLAVLCTAFAGWSVLTAGNTVPMPTPVVEPPRSPF
                     ETTVAASGIIEAANENVRIGPPSAGLVTKVFVAVGDAVREGDPLLQLDDRDLRAQLVA
                     RQAAIPPAEAQMEEQKYRIGDLDTQLKRLKSVRDSRAVSDDDVKRTWYAAEMAKRAMS
                     RFQAALKQVIAQRDETQLLLDRLTVRAPRAGTILQVNIRAGEFALTTGATEPLMLLGD
                     MQQLQVRAEVDEVNAPLVAPQRPAVAYLKGNTAQTIPLTFVRIEPYIVPKKSLTGDNN
                     ERVDTRVLQIIYRFERPAFPVYAGQQVDVFIERTPAGGPPQTQPVERRGEVSRK"
     gene            complement(5168..5875)
                     /locus_tag="PK589_16195"
     CDS             complement(5168..5875)
                     /locus_tag="PK589_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ21203.1"
                     /translation="MASVAVQVRGLVKSFGTGETTVTVLKGIDLDVHFGELLLLVGES
                     GGGKTTLLSAIAGILDIDAGDLQVLGASLTNMTPGTRTSFRGQRMGFVFQQFNLLPAL
                     TAAENVAIPLLIQGLRRPEAVSRGRLMLERVGLGDRTEFLPRNLSGGQQQRVAVARAL
                     VNDPQLLVCDEPTAALDGPNGQKIMELIRDVGRAPNRCVIVVTHDSRVFHFGDRMAEL
                     TDGRIVGIHPIHPEATA"
     gene            complement(5920..7050)
                     /locus_tag="PK589_16200"
     CDS             complement(5920..7050)
                     /locus_tag="PK589_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ21204.1"
                     /translation="MNYVALRMLFGDRAKYLMLLCGLTFAVMLIVQQGSIFWGLMMWS
                     QSSISNINVPIWVTDPGIGQVDEVKPIADTAVDRVRSVSGVEWAVPLYKGVLRARLSS
                     GDYHQITLTGLDAPTLIGRPAEVLEGRFEDILEPDAVVLDQWAVERMGGPSVIGIGTI
                     FELNDKLARVVAIAKTQKSFTNIPVVYTTYERAIRYVPRERRTLSYVLAKAKDGIPAS
                     EVTSRIHEQTGLGAFSAEEFGWKTISWVLKNTGIGINFGTTIILGFIVGMAIAGQTFY
                     LFTVENLKQFGALKAMGTSTFTLARMILLQAFTVGLTGYGIGIGLATGFGFLSAKGGG
                     LPFVETWQLLLLVLLALLGICTLSALISITKLARLEPAIVFR"
     gene            complement(7047..7463)
                     /locus_tag="PK589_16205"
     CDS             complement(7047..7463)
                     /locus_tag="PK589_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6022 family protein"
                     /protein_id="HNJ21205.1"
                     /translation="MKFIVSPLTPESVGREIQAYVNERQRQLLPSGEVTSEGRRFSWC
                     GDILDFLADGLAERLAPLGITFTNPFPGPLRLMDEVQQPDGQHVRRFWTVALHRGCPI
                     ARVCTLFFHRHDQISLPQVPLVMAFSPDHTAGEDGE"
     gene            complement(7639..9681)
                     /locus_tag="PK589_16210"
     CDS             complement(7639..9681)
                     /locus_tag="PK589_16210"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ21206.1"
                     /translation="MGTAFDTRDWFPILSALLQTNTLRQFDDRLQRELSQRLGCSVIG
                     LYLYSEMATAFSPVSESLRDPASPAYPIAQLPASGTIKEAAVLAGEAILTGDLSTSPW
                     TEARAIAPHISYGTSVLVSPIRMSCESVTGTAAKTLGVIAAVAPGKPGAFSEEDRIWL
                     DQLSRHIAPVLTAVLAAEERDTLVAINNQVVLGTLTLDNLIHSIQPILREVIHHDITG
                     LVRFVGEPHNRWFDIVACEGVDIDQDALRQFPFEQMAAAEILATGKPLLLTGHNQDRF
                     AEHRYFESLGVYSAMLCPLLVQGAPYGFLAIGSKRRNAFSERDLALTEQIGFHLSHAL
                     TNLSAYDQIRRLKDQLEQENVYLREEMGASLDLKSLVGDSPALQKSLRAIEMVAPTDS
                     TVLITGETGTGKELVAQAIHRLSPRRQKTLITVNCAALPPTLIESELFGHEKGAFTSA
                     TTRKLGRFELAHGGTIFLDEVGELPIDVQMKLLRVLEAQEFNRVGGSHTIRVDVRVLA
                     ATNVDLEQAIKRGAFRADLFYRLNVFPLRLPTLRERREDIPLLARHFAKKYGLRHRKP
                     VTRINSAALKALSAYDWPGNVRELEHLMERAVIVSRGSVLTIEELDGLGPSHERPSPP
                     RTLAEAERAHIVEVLVRTNWVLAGKQGAAETLGMKRSTLQHRMKKLDISRPPRHVS"
     gene            complement(9790..11364)
                     /locus_tag="PK589_16215"
     CDS             complement(9790..11364)
                     /locus_tag="PK589_16215"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HNJ21207.1"
                     /translation="MGQKWVESATQPATFPPPVRFREIDRANQEWPKRHRGQKMALTA
                     TEIGSIVQELASALADGWIQKISQPLPDTLILEIRVPGHTRRLLYSLRDDTARLHLVH
                     QHLPNPPTPPSFCQLLRARIQGARIDTLQHMSGDRIVRLDLTSRDGPASLVAELFSRN
                     ADLLFLDGEGLVLATLRHNKDRVGQPYQAPAAAPFRSSSHQAVVHEPDTQPQTDVDPF
                     PLSSRLEAFYREREAELSHQAQARERESGLRKHLKKLLRRMAALRQDLEQAGRYEPYA
                     RYGELLKANLGLLKKGQNRVAVVDYYDEQLPELTIPLDPTKGPQANMDAYFAKYRKFV
                     TAQREISPRIAAIEAEVQQILTELETIKQGTWQPPSTEGQTAARGTPALRRKRDREGE
                     SRGPFRRFVSSDGHPILVGRNARENDELTFGLAKSEDLWLHARGTPGSHVVVRLDKGT
                     EPPPETIRDAATLALLYSDLKKSGKGDVIYTRRKWVKKAKGQAAGAVTVTQEKSIYVT
                     LDKPRLDALKSRSAPR"
     gene            11577..11804
                     /locus_tag="PK589_16220"
     CDS             11577..11804
                     /locus_tag="PK589_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21208.1"
                     /translation="MTCTRCEGLMVMDDLVDLQESYHPMRLRGHRCLSCGNVVDTLIQ
                     RHRMVQNSHLRPVPASVPEKPVLFELIRRSA"
     gene            12045..13592
                     /locus_tag="PK589_16225"
     CDS             12045..13592
                     /locus_tag="PK589_16225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="HNJ21209.1"
                     /translation="MTVLDARMLERLMEEFRRSMQGLLTEVCRDFTKNYADAVSTFQL
                     PIDWFLSLGRSFPPAEFSNWKVVGWIESLNDLLYFVDIVTQVRQERSRRDIAEQLRAE
                     FREKFYEHGYTDEIFPNGKPEPRLLLGRLTALCHRLAREITQESVCLAPRLACAWVAK
                     QRRDAWLLPCDLTANVERVELPWGCAIGTTGVFYEATGAVRSALQRAKGQGEFLITAS
                     GIELLVGDRAYPLMTCGSQERWHWRRLDPLPLRDSRYGSLVLGPTLVYGKDKTPIGVR
                     PTKQEVAVRMRRALSAIAAAWPEGDRLLALLTSRIVPLKAAGVVSFSYRHRPGFSAIN
                     CFERDQLDLIDDLIHENSHHHLNLLLRNDVMYQHDHNQEVFYSPWRRTLRPLRGILHA
                     TFTFTMGAMLFERLVVWGSGTSGKAGWTKAGLTQRDLARARYRCLEEIESVRYSLQDL
                     EYAGKHLKWLTRGGKRLVTQLRDALTSMERGIQEYEQRVSASAFGPALRRHRIELAQA
                     RETLSLM"
     gene            complement(13730..14260)
                     /locus_tag="PK589_16230"
     CDS             complement(13730..14260)
                     /locus_tag="PK589_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21210.1"
                     /translation="MALIQQDLVAGSEDRLLTLDTETNLQWLNLSQTAGRSYQEIMNG
                     FGQFTTAHGFHYADGRQIGELCGHAGITKGLTEPALTPSPNDARNHQAIQTLQNLMDG
                     KVFHAETNVITSRGIMKPPAPPPNVPTRILGTIRLSLSLLNITGSHAESEGPTASPQT
                     GDPEIGSYLVRNQPEA"
BASE COUNT         2797 a         4063 c         4474 g         3123 t
ORIGIN      
        1 cgctggggcc tggtgaggga tgcgttgaaa tagttcaggc ctgactcgat gttgctcttg
       61 cccgggaatc taatcgaaag ccagacccaa gtcgccccgt tacgtcgcgt cttcggcccg
      121 tcctttccct ttctttcctt cgttcaagac aatgtcacta cccaagagga ggacccacat
      181 gggttctaaa atttatgttg gcgggttgcc gtattcggca accgaacagc agttgagcga
      241 tttgttcgca gtgcacgggg cggttgaatc ggcccgtatc attacggaca aatttaccgg
      301 ccaatcgaga ggtttcggat tcgtcgaaat ggcctcttca gacgaggcgc agaaggcgat
      361 ttccgctctc aatgggacgg acatgggtgg ccggacattg acggtcaatg aagcgcgtcc
      421 gcaggagcct cgttcaggcg ggcctggccg gagcggtgga ggcgggggat tcaacgatcg
      481 tggtggcaag cgcgatcgct ggtaacgcct gactttgtaa cagctcgtgg ggcagtcatc
      541 cttgtggtgg ctgccccatt tttttgtccg gatgagccgt gagggatttc tcgtacgcgc
      601 caattgggga tggtggtttt ctgtacgccg tgaaacgcgg aagatgacga ggcgagattc
      661 gcgagaagaa cgctgcgcga ctcgtaccat gtaagcgagg gtgcggggaa ggttacaggt
      721 taatcgagca ccttgagcgt gagatcgtca aaataggtga ccgcgtcggc tttggtccac
      781 agtccgatcc gtccgctggt gtacgtcgtg tcgcagacac gaaacagaac ggcgtcatcg
      841 aagagcgctt ttatttcgca gccgcgttgc acaatacgaa gtttgtgcca tccggtagcg
      901 gggagggggc gatcgacctg cttcacaatc tgctgcaccc cattcacgac gcgatagaca
      961 cggatttttt gctccaccag actcgctctc aacagatagt aatttctgtc gtccttcgcc
     1021 cgccacaaga gcccgccgcc gaagtcggct tttccggcca cggcgagata ggacacctcg
     1081 acatcgaggt tggcactctc ggtgccctcg aacaggagga gtttgtgcgt ctggtccgtc
     1141 cccttggcct gtagttgggc gagcacttgc gaggcgctct ttgcgcggtc ggtgatgagg
     1201 attttccatt ctcctgccgg tcggccatcg aacagagttc cgatgacgta tgaaccgggc
     1261 agcattcccg gggaagaact gtcaaacgtc caatgccgct ggtgctgtgg atcgctctct
     1321 gcttcctccg ctcccggcac tctgtcgggc catagagcgc cgacgcagat catcgtcatg
     1381 agcgagcctg cagcaatcac aagacgaaca aactgggcca tggtgtggtg cgggcgcatc
     1441 agagtccctg ttcagttaag gcgtttcgga ctgtacccta aacagggcgc tgtcggcgag
     1501 agggatgtcg aaaaattatc ttgttggtga tgatcgacct gtacgtagtg cgggaggtcc
     1561 aggactgctc cgccttttta cggcatttcc agttcgcgta gtttggcttc ggcctgttcc
     1621 aaccatcgac gattggccgg cgtgtcgagt tcgagccgaa ggtattgccg gagttcctgg
     1681 gcggcctcca tgcgttgccc aagaccggcg agtgcgtgcg caaggttgaa gcggatcttg
     1741 gcatcgttgg ggcggaggtg cagtgccgta cggaattcgg cgatggctgc atccggttga
     1801 tgcttctcca tcagcccgag ccccaggtta aagtgggcgt tcacatcgtt cggttgcaag
     1861 ctcgccgcag tgcgatattc tgcaatcgcc ccatccagat cacccatggc cttgagcgcc
     1921 acccccaggt tgttgtgggc attcacatcg gtgggattac ggcgtaacac ggttttgaat
     1981 tccgcgatgg cccccgccgg atcgcccatt tccttgagtg ccacccccag atcattgtgc
     2041 gtcttggggt catccggctt cagccgcaag gcttctttga attcaatcat ggcggcatca
     2101 tgccgaccga tatcaagtag tgtgttggcc agattcagat gcgcggcaaa atagttcggt
     2161 tggagccgga gggcatgtgt gaattcgtca atggcctggc ccacatcccc tttgaacacg
     2221 aaggcgacgc cgagatcatt gtgccgcatg gcctccgcgt cagtatcgat cggttgtgtg
     2281 atcttatcgg tggtcgagag aatctgctcc tcctccaaaa ggtttgtccc ggcgatgccg
     2341 gattttgccg cagaggtttc ctgcgaggcg gaagaggcct gtgtgctgaa tgggaggaca
     2401 agccccaatg ccaaaatgat cgaagcagga tacagggggc gtgccaaacg ctggccgcgg
     2461 acaccgtctc gcatgggagg ctccttccga gaagtcagag aggcattagc gtgaaaaggc
     2521 tggccggtgt ctggcctgcc gcttgatcgg gggtcaggga ttgcactgcg tcagatagcc
     2581 cgatctggcc ggaaaattca agttgaaagt ccgcggcgtc gcggtgcccg gctgatgata
     2641 tgagtaggga cgggggagaa agggtcaccg tgtcatcgaa ctcgatgccg ggacagaagt
     2701 gattccggga ggttgcgacc atccgccgcc caacgcttta tagaggctca ccatatcggt
     2761 gaggcgcgcc cgctccgttt gtgcgagctg tgtctccgcc gccagcatcg tccgctgcgc
     2821 atccaggaca tcgagataat tcaccagccc ctttcgataa cgcacttccg ccaggccaac
     2881 ggcggccgtc gcggcgaccg cctgctcacg ctggcgtgtc agttgctcgg cgcgggcttg
     2941 gatcgacacg aggagatcgg cgacttctcg aaaggcgagc agaatcgtct gttggtagcc
     3001 ttccaacatt tgttgatagc gggattccgc cgcatccagc cgtgcgacat tggtcccccc
     3061 gagaaaaatg ggcaaggtga cggatgggcc gatgctgaag ttggcgctgt tgccggtgaa
     3121 ccaattggca aactcggcgc tctgcaggcc tccttggccc gtgatggaga aggtgggaaa
     3181 gaaataggcc cgtgcctggc cgatacgggc attcgcggcg atgagagtcg cctcggcctg
     3241 caggatgtcc ggccgccgtt ccagcaactg tgcgggcagg ccgactggaa tgtcgggctg
     3301 gacgaccact tttctcagcg gcatcgatgc gagagtcagg ccgccgggtg ctgatccggt
     3361 cagcacttcc agccgatgga cttccaccgt acgcaagcga accaaatcgg gaatttgtcc
     3421 cgcgctttcg gcgacgagga cttccgttcg tttcacatca aggtcggagg ccaatcccac
     3481 ggacgcccgt ttggtgatga tctccaggga gtctcgccgc aaagccaggg cgcgttgcgc
     3541 gatttcgatc tgctcatcca actcgcgaat ccgaaaatag gcttgcccca catcgccgat
     3601 caaggtcagg gcgatcgccc gcgcatcctg ttcgatcgcc tgtgcatcgg ccgaggcagc
     3661 ttccattccg cgacggatcc gaccccacac atcgagttcc catcgcagat cggcggctcc
     3721 gttccaaagg tcaaaactgc tgcccggtct ggcgaagacc tgcgggcccg gttgctggcc
     3781 ggtggctaat ccaagtcccg ccaaggtatt tttcgagatg gagatgttgg tgtaactgct
     3841 ctgcacgttc acttgcgggt agagtcctgc tccggccgtg gtcactgatg cgcgtccctc
     3901 gatcacacgg gagaccgccc gacgcacatc gtgattgcgc gcgagggcgc gctcgacgaa
     3961 ttgcgtgagt tcctcgtttc gaaatgcttt ccaccaatcg gcgtcaggag tttttgggga
     4021 ggtgcctgag acgagagggc cggaggcgac atgagtccag tcggcgggcg tgtcaaccga
     4081 cggacgtgta taggccttcc cttgcaggca gccggttaac gacacggtca gtgtcatgag
     4141 gactgcggtg gccgcccgag atgcaagaat ccaccgattc atttccgcga cacctcaccg
     4201 cgacgttcaa cgggctgtgt ttggggcggc ccaccggctg gagtccgttc gatgaacaca
     4261 tcaacttgct gtcccgcata cacagggaac gcgggccgtt caaagcgata gataatctgg
     4321 aggacgcggg tgtctacccg ttcgttgttg tcaccggtca gggatttttt cgggacgatg
     4381 tacggttcga tgcgcacgaa ggtcaacgga atggtttgag ccgtgttgcc cttcaagtag
     4441 gcgacggccg gccgctgcgg ggcgacgagc ggggcattga cttcatccac ttccgcacgg
     4501 acctgcaatt gttgcatgtc gcccaagagc atgagtggct cggtcgcacc tgtcgtcagc
     4561 gcaaactctc ccgcccggat attcacctgc aggatcgtgc cggcgcgggg cgcgcgaacc
     4621 gtcagccgat ccagcagcaa ctgggtttca tcccgttgtg caatgacctg tttcagcgcc
     4681 gcctgaaacc gactcatggc gcgtttggcc atttccgcgg cataccaggt acgtttgaca
     4741 tcgtcgtcgc tgaccgcccg gctatcccgt acggatttca accgtttgag ctgcgtgtcg
     4801 agatcgccga tgcggtactt ctgctcttcc atttgcgcct ctgccggcgg gatggctgcc
     4861 tgcctggcca ccaactgagc gcgaagatcg cgatcgtcta actgcaagag cggatcgccc
     4921 tcgcgcaccg catccccgac tgcgacaaag accttggtca cgagcccggc agacggcggg
     4981 ccgatacgca cattctcatt ggccgcctcg ataatgccgc ttgccgcgac ggtggtttca
     5041 aatggagagc ggggtggctc cacgaccggt gtcggcatgg gaacggtatt gcccgcggtg
     5101 agtacagacc agccggcaaa tgcggtgcag agaactgcga gccagacact gacacgattg
     5161 agaatcgtca tgccgttgcc tctgggtgaa tcggatgaat gcccacgata cggccatccg
     5221 tgagttccgc catgcgatca ccgaagtgga agacccggct gtcgtgggtc acgacgatga
     5281 cgcagcggtt tggcgctcgc cctacgtcgc gaatcaattc catgatcttt tgtccgttgg
     5341 gtccgtcgag ggcggccgtc ggttcgtcgc agaccaggag ttggggatca ttgaccaagg
     5401 cgcgcgcgac ggccacacgt tgttgttgtc cccccgagag atttcgaggc agaaattcag
     5461 tacgatcgcc gagccccacg cgttccaaca tcagtcgccc gcgcgaaacg gcttcgggtc
     5521 tccgcaggcc ctggatcaac agcggaatgg cgacgttctc tgccgccgtc agcgcgggca
     5581 ggagattgaa ttgttgaaag acaaatccca tgcgctgtcc ccgaaaactg gttcgcgtgc
     5641 cgggcgtcat gtttgtgagc gacgccccca gcacctggag atcgccggcg tcgatatcga
     5701 gaatcccggc aatagccgac agcaatgtgg tcttgcctcc gccggactca ccgaccaaga
     5761 ggagtaattc gccgaagtgc acatcgaggt cgatcccctt tagcactgtc acggtggtct
     5821 cgccggttcc aaacgacttc accaggcctc gcacctgcac tgccacggaa gccatgccct
     5881 gttcctgcgc tacgctgtgt cctggaccat ccatcgacat catcgaaaca cgatcgccgg
     5941 ttcaagccgg gccagtttag taatgctgat caaggctgac agggtgcaaa tgcccaagag
     6001 cgccaggagt accagcagca gcaactgcca ggtttcaacg aagggcaatc cgccgccctt
     6061 ggctgagaga aatccgaatc ccgtggcaag gccgatgccg attccataac ccgtcagtcc
     6121 aaccgtaaag gcttgcagca gaatcatccg tgcgagcgtg aaggtggagg ttcccatcgc
     6181 tttgagcgca ccgaactgtt tcaggttttc gacggtaaac agataaaatg tctgtcccgc
     6241 gatcgccatg ccgacgataa agccgagaat aatcgtggtg ccgaagttga tgccgatccc
     6301 ggtatttttg agaacccagc tgatggtctt ccagccgaac tcttcggcgc tgaacgctcc
     6361 cagtccggtt tgttcatgga tcctggaggt gacctctgag gcaggtatgc cgtcttttgc
     6421 cttggcgagc acgtacgaca gcgtccggcg ctcacgcggg acatagcgaa tcgcgcgttc
     6481 gtaggtggta tagaccacgg ggatgttcgt gaagcttttc tgcgttttcg caatcgcaac
     6541 cacccgggcc agtttgtcat tcaactcaaa aatcgtcccg atgccgatga ccgagggccc
     6601 gcccatacgt tcaaccgccc attgatccag gacgacggca tccggttcca gaatatcttc
     6661 aaaccgtcct tccagcactt cggcgggacg cccgatgagc gtgggggcgt ccagtccggt
     6721 cagcgtgatc tggtgataat ccccgctgga gaggcgagcg cgtaacacgc ctttatagag
     6781 gggaacggcc cactccacac ctgatacgct acgcacccga tccactgcgg tatccgcaat
     6841 gggtttgact tcgtcgacct gtccgatgcc agggtcggtg acccagatcg gcacattgat
     6901 attgctgatg ctcgactgcg accacatcat gaggccccag aaaatcgatc cctgttgcac
     6961 gatcagcatg actgcgaagg tcagcccgca gagcagcatg agatacttcg cgcgatcgcc
     7021 aaagagcatt ctcagcgcca cgtaattcat tcgccgtcct ccccggcggt gtggtcaggc
     7081 gaaaaggcca tcacgagcgg cacctgagga aggctgatct gatcgtgtcg atggaagaac
     7141 aacgtgcaga ctctggcaat ggggcagccc cgatgcaggg cgacggtcca gaatctccgt
     7201 acatgttggc catcgggttg ctgcacttca tccatcaggc gcaagggccc tggaaatgga
     7261 ttggtgaaag taattcccaa tggggccaat cgctcggcga gtccatctgc cagaaagtcc
     7321 agaatgtctc cgcaccagga gaaacggcgc ccctcgctgg tgacctcgcc ggagggaagc
     7381 aattgacgct gccgttcgtt gacataggcc tgaatctcac gaccgacaga ctctggtgtc
     7441 agcggtgaca cgatgaattt catgggatcg acagaaagca tgcgccatgc cggatgaagg
     7501 gtggtggccc cagaacatca tatcgtgatg aaaatttgct ggaatactga agaggagagg
     7561 atgtggggtg gagtgaatct ctaatggtat ctgccgagat gttggcagat ctaaggagcg
     7621 gtgcccatat gcgggcagtt acgagacatg cctcggaggg cgactaatgt ctaatttttt
     7681 catgcgatgt tgcaaggtgg atcgcttcat gcctaatgtt tcagcggctc cctgctttcc
     7741 cgccagaacc caattcgttc gaaccagcac ctctacgatg tgtgcccgct cggcttcggc
     7801 caaagtgcgc ggcggggaag gccgctcatg gctcggaccc agaccgtcca gctcttcgat
     7861 ggtcagcacg gagccccggc tgacgatcac tgcccgttcc atgagatgct cgagttcccg
     7921 gacatttccc ggccagtcat aggcggataa ggctttcaat gccgcgctgt tgatgcgcgt
     7981 caccggttta cgatgtcgaa ggccgtattt tttggcaaaa tgacgagcca gcagcgggat
     8041 gtcttcgcgc cgttcccgca gcgtcggcag gcgaagaggg aagacgttca gacgataaaa
     8101 gagatcggca cggaatgcgc cgcgtttgat ggcttgctcc agatcgacgt tcgttgccgc
     8161 gagcacacgc acgtctacac ggatggtgtg gcttccgcca acccggttga actcctgcgc
     8221 ctcgaggaca cgcaagagtt tcatctggac atcaatgggc agctctccga cttcatcgag
     8281 gaagattgtt ccgccatgag ccagttcgaa ccggcccagc ttgcgggtcg ttgcactggt
     8341 gaaggcaccc ttttcgtgcc cgaacagttc ggattcgatg agcgtgggcg gcagtgccgc
     8401 gcagttcact gtgatcaggg ttttctgccg gcgcggggac aaccggtgaa tggcctgagc
     8461 caccagttcc ttgccggtgc cggtttctcc cgtaatcagc accgtggaat ccgtgggcgc
     8521 gaccatttcg atggctcgaa gcgatttctg aagtgcgggg ctatccccca cgaggctttt
     8581 cagatccagg gaggcgccca tttcttcacg caggtacaca ttctcctgct ccagttgatc
     8641 cttgagccgt cggatctggt cataggcgga gaggttggtg agggcatggg acaggtgaaa
     8701 gccgatttgt tccgtcaacg ccaggtcgcg ttcggaaaat gcgttgcgcc gtttgcttcc
     8761 gatggccagg aaaccatagg gcgctccctg gaccaggagc gggcagagca tggcggagta
     8821 gaccccgagt gattcgaagt accggtgttc ggcaaatcgg tcctggttgt gtccggtgag
     8881 cagcaggggt ttgccggtgg cgaggatttc ggcagcggcc atctgctcga acggaaattg
     8941 ccgcagggcg tcctggtcga tatcgactcc ctcgcacgcc acgatgtcga accaccgatt
     9001 gtggggttcg ccgacaaacc gtacgagccc ggtgatgtcg tgatggatga cttcgcggag
     9061 gattggttga atcgagtgga tcagattgtc gagcgtgaga gtgccgagca ccacttgatt
     9121 gttgatggcg accagcgtgt cccgttcttc cgcggcgagc acggcggtca gaaccggggc
     9181 gatgtgccgg ctcaactggt cgagccatat ccgatcctcc tcggaaaacg cccccggttt
     9241 accgggtgcg accgcggcga tgacgcccag cgtcttggct gctgtaccgg tcaccgattc
     9301 gcatgacatt ctaattggtg agacgaggac cgatgtgcca tagctgatat gaggtgctat
     9361 ggctcgggct tccgtccagg gtgacgtgct gagatcgccg gtcaggatcg cttccccggc
     9421 caggaccgct gcttccttga tggtcccgga cgcgggtaat tgcgcgatgg gataggcggg
     9481 cgacgccgga tcacgcaagc tttccgagac gggtgaaaac gcggtcgcca tctcactgta
     9541 gagatagagg ccgatgacgc tacagcctag gcgttggctg agttcccgtt ggagtcgatc
     9601 gtcgaactgg cggagggtgt tggtttgaag gagcgcgctt agaatgggaa accagtcacg
     9661 tgtgtcgaac gcggtgccca tatgtgggca ctctaccatg tcgtggtgag tgatgggaag
     9721 acggagaggg acggacagga attgtatcgc tcagggtgaa atgaaggatt caggatggcc
     9781 gaatctgcgt catcgcggtg cgcttctgga tttcagggca tccaggcgcg gtttatcgag
     9841 tgtgacatag atagattttt cctgcgtcac ggtcacggcg cctgccgctt gccccttggc
     9901 ctttttgacc cacttgcgcc gggtatagat cacgtcacct ttcccgctct tcttcagatc
     9961 gctgtagagg agggccagtg tggccgcatc tcgtatggtt tcgggagggg gctcggtccc
    10021 cttgtccagc cgaacgacca catgcgagcc gggcgtaccg cgcgcgtgaa gccagaggtc
    10081 ttcgctcttc gccagtccga aggtgagctc atcattctcc cgggcgttgc ggccgaccag
    10141 gatgggatgg ccgtccgatg agacaaaacg gcggaacggt ccccgggatt ccccctccct
    10201 gtcgcgcttc cgtcgtaggg caggggttcc tcgtgccgcg gtttgtcctt ccgtgctcgg
    10261 tggctgccag gttccctgtt tgatggtctc caattcggtt aggatctgtt gaacctctgc
    10321 ttcgatcgca gctatgcggg gcgagatttc gcgttgcgcg gtcacgaact tccggtactt
    10381 ggcaaagtac gcgtccatat tggcctgcgg ccctttggtc ggatcgagcg gaatcgtcag
    10441 ttctggcaat tgttcatcgt agtagtcgac gaccgccacc ctgttctggc ccttcttcag
    10501 taagccgaga ttggccttca acaattcgcc gtaccgtgcg tagggttcat atcgtccggc
    10561 ctgttcgagg tcttggcgga gagccgccat gcggcgcagg agctttttga gatgttttcg
    10621 cagtcccgat tcccgttcgc gggcctgagc ctggtgcgac aattcggctt cgcgctctcg
    10681 atagaacgcc tcgagtctgg atgacagcgg aaatggatcc acgtccgtct gcggctgcgt
    10741 atccggctcg tgaacgaccg cctgatggga tgacgatcga aacggtgcag ccgccggcgc
    10801 ctgatagggt tgacctacgc ggtctttatt gtgacgaagc gtagcgagca ccaggccttc
    10861 gccatcgaga aagaggaggt ctgcattgcg gctgaaaagt tctgcgacga gagaggcggg
    10921 tccgtcccgg ctggtgagat caagtcgaac gatccgatcg ccggacatgt gttgaagggt
    10981 atcgatgcgg gcgccttgga tccgagcccg gagcagctgg cagaacgaag gtggggtcgg
    11041 cgggttgggt agatgctgat ggacgagatg cagccgcgcc gtgtcatccc gcagtgagta
    11101 gagcaggcgg cgagtatgtc cggggacacg gatctcgagg ataagggtgt cgggaagcgg
    11161 ttgggaaatc ttctgaatcc acccgtcggc gagcgctgat gccagttcct ggacaatgga
    11221 accaatttct gtcgcagtaa gagccatttt ttgaccgcga tgccgcttcg gccattcctg
    11281 atttgcccgg tcaatttctc tgaaccgtac cggaggaggg aacgttgccg gttgtgtagc
    11341 cgattctacc catttttgtc ccatgagacc aattcggtca caattagctc aattcccacg
    11401 agccgctcac gtttgagagc aggggcctca tccggccaaa tcgtcctctg caccccagag
    11461 tcggtggcat ccacattgct tctctccata gcaccaggtt ggtgagttgt acgaatgggc
    11521 aacagatcgt cccgtcttga cggggatctc agaggctgtt cgaagggagg cgactcatga
    11581 catgcacgcg atgtgaaggc ctaatggtga tggacgattt ggtcgatctc caggaaagtt
    11641 atcatcccat gcggttgcgt gggcaccgct gcctcagctg cggtaatgtg gtggatacgt
    11701 tgattcaacg acaccggatg gtgcagaact cccatctccg cccggtccct gcctctgtcc
    11761 ccgagaagcc ggttctcttt gagttgatca ggcgctcggc gtaacgcgtt tccccgcgaa
    11821 gccgctccgg tttcgcgggg ctgttctcca cgcatgacgc gcgtgggcgt atcccacggc
    11881 gccctgcgtc tccatatctt cactgctgct tcccctccac ccgcgcaatt ctccccaatg
    11941 tgtcatgggt gttcactgct tgcagtcatg ttgcatgctt gtcagcgatc aagcagtcgc
    12001 gtagagtgcg cccggctctc ttcgattcag aaaggcgatc agcgatgacg gttcttgatg
    12061 cgcgcatgct tgagcggttg atggaagaat tccggcgatc catgcaaggg ttgttgacgg
    12121 aggtgtgccg cgatttcacg aaaaattatg ccgacgcagt cagcacgttt caattaccga
    12181 tcgactggtt cctgtcgctc ggacggtctt tcccgccagc cgagtttagc aactggaaag
    12241 tggtgggatg gatcgagtcg ctgaatgact tgctgtattt cgtcgatatc gtgacccagg
    12301 tccggcagga acggtcgcgg agggatattg ccgaacagtt gcgcgcggag tttagggaga
    12361 aattctacga gcacgggtat accgatgaga tttttcccaa cggcaaaccg gagccgcgac
    12421 tccttcttgg gcggttaacg gcgctctgtc accggctggc tcgggaaatc acgcaagagt
    12481 ccgtgtgtct ggctccccgg cttgcctgtg catgggtggc gaagcaacgg cgggacgcct
    12541 ggctgctgcc ttgtgatctt acggcaaatg tcgaacgagt ggaattgccc tggggctgtg
    12601 caatcgggac gaccggtgtg ttctatgagg cgactggtgc ggtccgctca gcactgcagc
    12661 gcgcgaaagg ccaaggggaa tttctgatca cggcctcagg gatcgagctg ttggtcggcg
    12721 atcgtgccta tcccctgatg acgtgtggca gtcaggagcg gtggcattgg cgtcgcctcg
    12781 acccgttgcc gcttcgggac agccggtacg gatcactcgt ccttgggccg acactcgtgt
    12841 atggaaaaga caagacgccg atcggtgtgc gaccgaccaa acaggaagtc gccgtgcgca
    12901 tgcgccgggc gttatcggcc attgccgcgg cctggccgga aggagaccgg ttgctggcgt
    12961 tgctgacctc gcgcatcgtg ccgttgaaag ccgcgggtgt cgtcagcttc agttaccggc
    13021 atcgtccggg gttctccgcc atcaattgct ttgagcgcga tcaactggac ctgatcgatg
    13081 atttgatcca cgagaatagt catcatcacc tcaatctgct gctgcgcaac gatgtgatgt
    13141 atcagcacga tcacaatcag gaagttttct attcgccctg gcggcgcaca ctgcgcccgt
    13201 tgcgcggaat cctgcatgcg accttcacct ttacgatggg cgccatgctg ttcgagcggc
    13261 tcgtggtatg gggatcaggt acgtcaggaa aagccggctg gacgaaggcg ggcctcaccc
    13321 aacgcgacct cgcccgtgct cggtaccgtt gcctggaaga aatcgaatca gtccgttatt
    13381 cgctgcagga tctcgagtat gcgggcaagc atctgaagtg gcttacaagg ggcggcaagc
    13441 gactggtaac gcaattacgc gatgcgctta cctctatgga acgaggaatc caggagtacg
    13501 agcaacgcgt cagtgcatcc gcgtttggtc ccgcgctgcg ccgtcatcga atcgaattgg
    13561 ctcaggcgcg agagacgttg agcctgatgt aatcccctca tggactcggg agtgaggtgg
    13621 ctgcagaacg ggccccggcg tcagttcacg ccggggcccg tgtactgtgc gcatgaatcg
    13681 cgtcgtgccg tcttgttgtc agaccacacg tctcccgttg ttggtgcgct cacgcctccg
    13741 gctgattccg aaccagataa gaaccgattt ccggatcgcc ggtctgtggg cttgcggtcg
    13801 gtccttcgct ttccgcatgg gagcctgtga tattcaagag gctgagagac aatcgaatgg
    13861 ttcccagaat tctcgtcggg acgttcggtg gcggagccgg aggtttcatg atgccgcggc
    13921 tggtgatcac gttggtctcg gcatgaaaca ctttgccgtc cataaggttt tggagggtct
    13981 ggatcgcctg gtgattccgg gcatcattag gggaaggcgt gagggctggt tccgtcaagc
    14041 ctttcgtaat gccggcatgc ccgcataact cgccgatctg acgaccgtcg gcatagtgaa
    14101 acccgtgcgc cgtcgtgaac tggccgaatc cgttcataat ctcttgatac gatcgcccgg
    14161 cggtctgcga aagattcagc cactgtagat tcgtctcggt atccagcgtg agcagccgat
    14221 cctcgctgcc tgcaacgagg tcctgttgaa tgagtgccat gaggaaccct ccatggttgg
    14281 cggtaggaga cggtattcaa ctagatgagg gtgatactag cacaagggca tcgcgcgaac
    14341 gtcacttccg ttccggcaga catgcagcgc actgtatgcg gtctttatga tggaggggtt
    14401 ggaaggctgc aaggtctgta tcggagaaga tacgagcgta tccccgcaga tacagag
//
LOCUS       DAOQDS010000086        14190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1882_length_14190_cov_20.463813, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000086 DAOQDS010000000
VERSION     DAOQDS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14190
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1882_length_14190_cov_20.463813"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1399..2001
                     /locus_tag="PK589_16235"
     CDS             1399..2001
                     /locus_tag="PK589_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21211.1"
                     /translation="MNVKEEGAAESDGSKGRIFAGGIGADSQGKSGFMLRVYDAETGR
                     FLWEGQLNLLKVNEEGPSKVKAVIAPKPAVVIPASDRPPQVFQTFFSVRAVNPLTGRL
                     VWQDQFTPGTRKRPGSAGVSCKRCSVSPVNEPIGHIFDLVVRTYDRTSGLLLWEDSFE
                     EIDRIDESDSAPETSAYPQAIPFWHPGVADKTPMYKVTLR"
     gene            complement(2005..3444)
                     /locus_tag="PK589_16240"
     CDS             complement(2005..3444)
                     /locus_tag="PK589_16240"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21212.1"
                     /translation="MYRRNIKHILGPLAILIAIFIFYQSWLKPHYFHEMPAPPPQVIT
                     EEVAGSKGPVAPPQQAVLPEMKRSRTIDPVSIPVPRHSELLGTIHDELEKHNIALAQT
                     KLEELPSSILSESATKRHVAILWNNLGILQENAGGTETSVKAFKKAVMLDPQNPVAHL
                     NLAHAYWGLRDPALTEEFLKKVLTLSPDEPFPHVALADLLQEKDRLSEAAKHLAQAMD
                     RIKKDPGLQSYVKVVTAKVHRAEKVEEKFSTRNSVHFTVKYDGSEDPETWTTVLDILE
                     AAYRDIGQKFNFFPSKPIMVVLHTKTQFQGSTGSPVWADGLFDPVLGRIQIPSQGATT
                     DRAWLTRVLRHEFVHALIHEELGATGGAIPTWLNEGLAMQLANDSWQEVANMPPGEHI
                     LIPLAALEEGWENLSAEKVGAAYMEANAATHYLIERFGMHKVHELLLHLKARQTIAAA
                     MQDRLLLSYDHFQQQWVESMGATLSTPKS"
     gene            3666..>4147
                     /locus_tag="PK589_16245"
     CDS             3666..>4147
                     /locus_tag="PK589_16245"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21213.1"
                     /translation="MARPIEFACRDAYAHLPTVKNLDRFVSEQVIGALGERLHPRALE
                     VELLSLRNLFVDFHAGLTAAEQQHRLTQALQLLRRLQGQSPMVHPSADLLEGGELVHT
                     PVNIHSRQLSIESSIQFAKGVGPKRTLLLKRLGIQTIEEAIWTLPWRYDDRSTITPIA
                     "
     assembly_gap    4148..4247
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4248..6304
                     /gene="recG"
                     /locus_tag="PK589_16250"
     CDS             <4248..6304
                     /gene="recG"
                     /locus_tag="PK589_16250"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNJ21214.1"
                     /translation="WTLPWRYDDRSTITPIAKLMLAATVCICGVITRTEASRARSRRL
                     SILDVVVQDVTGTVHAVFFNQPYLEDVLKEGARVMMAGRVVAGRRGWTDLRLEPTQFE
                     ILSGQEDELLHVGRIVPIYHETKGWTSRQMRVLIQGVLAGHGAELVDVLPLSIRARHR
                     LPPIGEAIQQVHFPPPQTDLAALDRGVTAAHRRLAFEELFLLQTAMVLRQQQVKDEEK
                     PFRFSPHVPELKKLSQILPFSLTAAQERVWREIQADMATSRPMNRLVQGDVGCGKTVV
                     ALHALVMACGSGCQTALMAPTEILAEQHYLNLRPLLEAVGFKAVLLTSGGKAKDRQAV
                     FTQLASGEAQVAIGTHALIQKNVAFDKLGLVVVDEQHKFGVLQRKSLLDKGYRPDVLV
                     MTATPIPRTLAMTVYGDLDVSVIDMLPPGRKPVRTMLYSEGQRHKTWQLVSDELKAGR
                     QAYIIYPLVEESEKIDLKAAIQGAEQLQRDIFPQAQIGLLHGKMPSSEKERTMAAFKA
                     GTIQILVATTVIEVGVDVSNATVMVIEHAERFGLAQLHQLRGRVGRGAHQSLCILMAS
                     YLPRESRPKVSAEGKPEANASNAQQRLSALVKSHDGFVIAEEDLRIRGPGEFLGLRQW
                     GLPEFRAANLIRDTHLLELARQEAFAMVTQDPGLALPQHQAVKAAMFRRWKGKLSLGD
                     VS"
     gene            6349..6675
                     /locus_tag="PK589_16255"
     CDS             6349..6675
                     /locus_tag="PK589_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21215.1"
                     /translation="MGMLQRLSHDLRAGWVTLRHGTAKAATRALEEGELLGYRLELRK
                     VEQQLDDLHVDIGERTIELHDRGEAPDRIVADSEVARLMKQVQALQDERTKLLLEMND
                     ITVEEA"
     gene            6684..7655
                     /locus_tag="PK589_16260"
     CDS             6684..7655
                     /locus_tag="PK589_16260"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNJ21216.1"
                     /translation="MAGASTGSSRILAGIDIGTLTCRLEIAEVTSSGQVKKLRSDRRI
                     LRLGQGVDRDRMLRTDAMARVVTTLKEWRQSIDTYQVEATSVVATSAVRDAGNRQDFL
                     DLIAREAGLQVEIISGEEEARRTTLGIRSGLPEGVSDMLAVDIGGGSTECILDRPGQP
                     LIVRSIDIGVVRLSERVLQSDPPTAEELQQAHDWIRTETGAAIADMPLPVGLTFVGTA
                     GTITALAAMEQQLPVYEAAKIHNYVLTLEAVKRLEAQLLGRSKQQREGLPGLERGRED
                     VIVAGAIILSTMMETLGMTKVLVSDLGLREGVLLHLASRLNGGSSLR"
     gene            complement(7710..9203)
                     /locus_tag="PK589_16265"
     CDS             complement(7710..9203)
                     /locus_tag="PK589_16265"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ21217.1"
                     /translation="MKAAAAIQEPLNLSGDILSLLAWRIPDLVAYQHAFDEWWLAPLD
                     DDFPLVRLNAVGIEMLTSMNGHITVGALVEKYGNKICGPDGQPGSWHLAHWSIPNYSL
                     CYFGTEPPGGHRHKAKWDLLLQQIRESWSGQQDFEGEEHLEDFHVHELKESDLEDGHF
                     DLIETTVSHLFREPCEALGGTTYGRLLMRQLRRLGWFKPKPKVIVEIGGGLGYVAREL
                     GQELLPFEKQGIQYISLDVTRPFLQLQTKRAKAGGWTGTGTHANGEWLPFKDNSVDLI
                     IDNENMADMTPVKLSREELTSGRGETAQHQEALDWIRRLRLPLDKELPEEVIFNLGPM
                     RFVAEVWRVLKPGGRAFLTEFGIEEGWPAAVKLPHHTEYEVQYSHLRQAVRWLGFQER
                     YLSLPQFLQLKPDTKVLCTGAAYTIQRFCQGLGQHFSVRAYTESELTKTLGDTLPKLQ
                     GCHYHDIADPAWFGLIDFKVLLLEKPGGMPQATFTEQKSGLRWYSQR"
     gene            complement(9285..9659)
                     /locus_tag="PK589_16270"
     CDS             complement(9285..9659)
                     /locus_tag="PK589_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNJ21218.1"
                     /translation="MSWRTLSLSLTFAFLMAWPSSATPPAVHVLMDSGSPYYVPAAAT
                     VTAGGAIRWDNPTPTHHTVTHDGCFDESGRCAFDSGAVEPGGTYTIPSLPPGRYPYQC
                     RIHPIMRGIIIVTDSPLLPSQT"
     gene            9956..10561
                     /locus_tag="PK589_16275"
     CDS             9956..10561
                     /locus_tag="PK589_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21219.1"
                     /translation="MAKKTMKKKSSAVKVARPKKGAAFKASKPKKVVKAKKAAPKKPV
                     KAKKAAQPKVTAKKPVKKTAKKSTAKTAPAKAARSSKAKATPKAAKPAPKPASKPKTK
                     PASPKAKTPAEAPAAVPESKPVKSSVPEPEKASNVEMEPTQLDLEDDEEDMAEDLDDV
                     SEEEMEEEIEEELGLDNEDDDVDYLEKSEDLLDDADEYRNN"
     gene            10773..11225
                     /locus_tag="PK589_16280"
     CDS             10773..11225
                     /locus_tag="PK589_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21220.1"
                     /translation="MSHFLFIAGALRERTSPESAELQLGAGIWGLCTSLIRDNLQKFL
                     TPESHGLVYVLKEGIRAEFSIVSPVQPFQALDELLKDELRTEARYGFVQVTPIRRWEG
                     NAPECQQLLQRVLGIEEPSELSRRLSLGMHRLSQEEYRAIVDGLGAEH"
     gene            complement(11253..>11825)
                     /locus_tag="PK589_16285"
     CDS             complement(11253..>11825)
                     /locus_tag="PK589_16285"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21221.1"
                     /translation="ESVVEVPMLAAIQSNQLGVFEVLLLRWDHQPTPSPIMLQWQGGN
                     VKPGQTNLGSMVLALQYALEHTPTVNHTGTISVIGISYAATGTDGPSAGGVMTVGFAA
                     LLKGEQIRRGIAMTGTISKDGTIGPVGSIPDKIRAAAREGYRTILIPQGQRDDPRWNL
                     TRLAWDLNVEIKEVATVDEAYHLMTGGTLD"
     assembly_gap    11826..11925
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11926..12972
                     /locus_tag="PK589_16290"
     CDS             <11926..12972
                     /locus_tag="PK589_16290"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNJ21222.1"
                     /translation="GILWGTVMLPGLLFAECTSETGGKTASAVFTLHLTGTCTEAERE
                     ARAVPAKDLMRALAAGKGIDLAGVVIQGDLVLDELPAQKVDAVQGLSLEDRRVLEGLN
                     DEEVHVIRGPFVIKHSRVKGQIVNRLKRGFLLITGPVVLVHSGFDGLVDLSRTVFLGL
                     VDGSNAKFEKESYFVQDRFTQGAMFSDTRFGSHARFHRSMFSGPAIFRGADFPGLTEF
                     LEVVFEQDANFARTAFHMGTGFSGAHCRGKCDFSSTLFEREAFFLFARFDRAVTFASA
                     KFSSQADFSDASFKEADDLAKATFVRPPVMIRTARVVSTVPVAPEAGPFSQVVTIGLF
                     VAALGILIYILKAK"
     gene            13068..13847
                     /locus_tag="PK589_16295"
     CDS             13068..13847
                     /locus_tag="PK589_16295"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNJ21223.1"
                     /translation="MDQPELPYQVKIENFEGPLDLLLHLIKKNEINIYDIPIALIAQQ
                     YLDYLSVMKELNLAVAGEFLVMAATLLHIKSRMLLPTDEVAVDEEDGPDPREELVRRL
                     LEYKQFKEAASQLDYKERLWRDTFGREPGPPIEIAKDESLLDEISLFDLVDALQGVLS
                     RHPGKRLVEIIPDNLTVRTRMSAIIEALEVHESMPFTALFEESSHRLVVIVTFLALLE
                     LIRIRVVRVFQSETFGAILVSRAFTAVAEGDLVEESEWKNL"
     gene            13832..>14190
                     /locus_tag="PK589_16300"
     CDS             13832..>14190
                     /locus_tag="PK589_16300"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNJ21224.1"
                     /translation="MEEPMSLSMEDAVEQPAEVAEVEAPVSEEPTAEPEVEAHAGEGD
                     VAEMAAPTPASDPALSDVRALKGILEALLFVTPDPIPVTRFLALLGAVTKQEVDQALA
                     SLSHDYEQEGRGLQLAEV"
BASE COUNT         2981 a         3615 c         4298 g         3096 t
ORIGIN      
        1 tcaaggcagg gttcgctgag cagacggaaa tgaacgacga atatgtcgcc atcacgttcc
       61 tcaaggcggt gaatttcgaa cgctacgagg aggttcttgg ctccgtgcgt tggtgtcaga
      121 atcttttcaa gacgtaatct tcacgcaccc taagggcgcc aattccccct gtttgcctct
      181 tttccctgtc cgatgctctc ccttcagcct atccgtgctt tgctcgccgc gcataatcat
      241 ggtcaagtag aaccgcaccc tcttcccatc aacgggagcc ggaagaatgc ttggtaagcg
      301 cgtgcaagct ggatcctttg ccaaaatctt gccctgtcag gacttggcct cagtgcgggt
      361 ctccgccagc agaggaattg cgatgtgggg tgctggaggg taggtgaatt acgacgagga
      421 agaagatcga tattcggact gatggctttc tgcaggccgt tgactgggct gataagtgag
      481 catgcaccgt cctcccataa tcataattag gaaattgact agactgcttg ctaagacaag
      541 gatgaggaca accaggcgag gagatccttg gctggcttcc tgaacgaccg tggcgaggtc
      601 gagtgcgagg gcaatagtgc tgtatatgac cgctcccatc acggcccacg gctgacggat
      661 gaagatcagt cccccgagca gcagcgcggg taaaacgaat agaaacccga ctaagatgcc
      721 gtctttgatc gtcgagagag gggagcctga ttcaactcca aagagaatac cctcaaggac
      781 aatgagaaca ctcaacagga cgacgatggg acgcgcatgg agcatagcgg tactataacg
      841 gtggcatatc cctctcgcaa gctatcagat tccgcccata tcttagtgcc gggtcatcat
      901 caaagataat gatcgatcct cggggtacgc tgggcaaggt caatgacggc taccttcccc
      961 atccttcgag gggaccacca ggtccgttga gtcatttacc ctagatccgt attcacgtga
     1021 aaatccgcct ccattaggga ggcacacgtg gcccattaca catctcacgt acgagttcaa
     1081 catcggggag ccagagcatg gtggcccgca acagtcacct cgcagcgctc tcttgccatc
     1141 gggcgatcgt tacccctgct gctgtgcttg atggtagcgg tgagttgcct gccgggcctt
     1201 gctttggcgg aagtgtatga ctttccggct cggggccata ggggcggggc catcgctgac
     1261 ccagtggtac aaaggccagc cgaccattcg tcggtactag cggcaggatt tggttttcag
     1321 gcagcaggat cgtccctcat aacggtccga acctatgatg ttcagacagg cgccattctt
     1381 tcagaggatt catttgacgt gaacgtgaag gaagagggcg ccgctgaaag cgatgggagc
     1441 aaggggcgta ttttcgccgg aggaatcggt gccgacagtc agggaaagtc tgggtttatg
     1501 ctgagagtat atgatgcgga gacaggaaga tttctctggg aagggcaatt gaacttgctc
     1561 aaagtgaacg aagagggacc gagtaaagtg aaggcggtga ttgctcccaa acctgctgtc
     1621 gtaatacctg cctcagatcg ccctcctcaa gtcttccaaa cgtttttctc cgtgcgagca
     1681 gtcaatcctc ttaccgggag gcttgtttgg caggatcaat ttactccagg cactagaaaa
     1741 cgaccgggca gtgcaggggt gtcatgtaag aggtgctcgg tgagccctgt caatgagcct
     1801 atcggacaca tttttgactt ggttgtgcga acctacgatc gcacatcagg cctgctgttg
     1861 tgggaggatt cgttcgagga aatcgatcga attgatgagt ctgattccgc gccggagaca
     1921 agtgcctatc cacaggcaat accattttgg catccggggg ttgcggacaa aacccccatg
     1981 tacaaagtga ccctccgctg acagttacga tttgggcgtg gataaggtgg cgcccatgct
     2041 ctccacccac tgttgctgga aatggtcgta ggagagcagc aggcggtcct gcatggcagc
     2101 ggcgattgtc tggcgcgcct tcaagtgaag aagtaactca tgaaccttgt gcatgccgaa
     2161 tcgttcaata aggtagtggg tagcggcatt ggcttccatg tatgcggcgc ccaccttttc
     2221 ggccgataag ttttcccagc cctcctcaag agccgccagg ggtatcaaga tatgttcccc
     2281 cggaggcatg tttgcgacct cctgccagga atcattggcc agctgcatag ccaacccttc
     2341 attgagccag gtgggtatgg ctccaccggt tgctcccagt tcctcatgaa tcagcgcgtg
     2401 gacaaattcg tgacggagaa cgcgagtcag ccaggcgcga tctgtcgttg cgccttgact
     2461 ggggatctgg atgcgcccca gaacagggtc gaataagcca tctgcccaca cggggctgcc
     2521 ggtggatccc tgaaactgag tcttggtgtg cagcaccacc ataataggtt tcgacggaaa
     2581 gaaattgaac ttctggccga tgtctcgata cgccgcctcg agaatatcca gcacggttgt
     2641 ccaggtctcc ggatcctcac tgccgtcata cttgaccgta aaatggacgc tgttacgcgt
     2701 tgaaaacttc tcttctacct tttccgcgcg atggacctta gccgtcacga ctttcacata
     2761 agactgcagc cctggatcct tttttatgcg atccattgcc tgtgcgagat gtttggccgc
     2821 ttccgacaag cgatcttttt cctggaggag gtccgccagc gccacatgcg ggaacggctc
     2881 atccggagat agggtcagca cctttttcaa gaattcttcc gtcagcgctg gatcccgcag
     2941 accccagtag gcatgggcaa ggttgagatg ggccaccggg ttttgggggt ccagcatcac
     3001 cgccttctta aaggctttga cggaggtttc agttccgccg gcgttttctt ggagaatacc
     3061 gaggttgttc cacaagatgg ccacatggcg ttttgtggcc gactcagaaa gaatcgacga
     3121 ggggagttct tcgagtttcg tttgtgccaa tgcaatattg tgtttctcaa gctcgtcgtg
     3181 aatggtgccg agcagttcgg agtgccgagg gacgggaatg ctgacgggat caatggttct
     3241 ggatcgtttc atttccggta gcaccgcttg ttgcggaggc gccacgggac ctttggatcc
     3301 ggcgacctct tctgtaatga cttgaggtgg cggtgctggc atctcgtgga aatagtgcgg
     3361 cttgagccag gactgataga aaatgaagat agcaatgaga atggccaacg gcccgagaat
     3421 atgcttgata ttgcgtcgat acataggtgt ccttccgtga gtcctacacg aagttactct
     3481 agcatcgagg tagtggacga ccaaggaaag tcgcaaaagc gggcgaatgg tggtcacggg
     3541 agggtgtgtt cgtcatcgcc ggtccttcat tgagcaggca ggcagcctat ggtacgatcc
     3601 cgcctaggat gccacgtcct gattcttctg atccccatga gtctttccgg tcattcctgc
     3661 agcgagtggc aagacccatt gaatttgctt gccgtgatgc ctatgcccac ctgccgacag
     3721 taaaaaatct tgaccgtttt gtgtcggaac aggtcattgg cgcgctcggt gagcgacttc
     3781 acccgcgcgc gcttgaggtt gagctgctct ccttacgcaa tctgttcgtc gattttcatg
     3841 ccggactcac ggctgccgaa cagcaacatc gactgacgca ggccctccag ctccttcgcc
     3901 ggctccaagg gcagtcgccg atggtgcatc cgtccgccga tcttctggag ggaggggagc
     3961 tggttcacac tcccgtgaat attcattcca gacagctgtc tatagagtca tcgattcagt
     4021 tcgcgaaggg tgttggtccg aagcgcaccc tcctcttgaa acgtctcggc atccagacga
     4081 tcgaagaggc aatctggact ttgccatggc ggtatgatga tcgatcgacc atcacgccga
     4141 ttgctaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntct ggactttgcc
     4261 atggcggtat gatgatcgat cgaccatcac gccgattgct aagctgatgt tggcggcgac
     4321 ggtctgtatc tgtggggtga tcactcgaac ggaagcttcg cgcgcacgct ctcgccggtt
     4381 gtcgattctg gacgtcgttg ttcaggacgt aactggaacg gtgcacgcgg tgttctttaa
     4441 tcaaccctat ctggaagacg tgttgaagga gggggcacgc gtcatgatgg ccgggcgtgt
     4501 agttgccggt cggcgaggat ggacggatct tcggctggag ccgacgcaat ttgagatatt
     4561 gagcggtcag gaagatgaat tgctgcacgt cggacgcatc gtcccgattt accatgagac
     4621 gaagggctgg acctctcgcc agatgcgcgt cttaatccag ggggtactgg ccgggcacgg
     4681 tgcggagctt gtggacgttt tgccgttgtc cattcgcgcg cgccaccggt tgccgccaat
     4741 tggcgaggct attcagcagg tacatttccc gccgccgcaa accgacctgg ccgcgcttga
     4801 ccgcggggta acggctgccc atcgccgatt ggccttcgag gaattgtttc tgcttcagac
     4861 ggccatggtg ttgaggcaac agcaggtgaa ggacgaagag aaaccgtttc gattcagtcc
     4921 tcatgtgccg gaactcaaaa aactgtcgca gattttgccc ttctcactga cggctgcgca
     4981 ggaacgggta tggcgagaga ttcaagccga tatggcgacc tcgcgcccca tgaatcgtct
     5041 ggtgcagggg gatgtggggt gtggaaaaac ggtcgtggcg ttgcatgccc tggtgatggc
     5101 ctgtggctcc ggttgtcaaa cagcgctgat ggctcccacc gaaattctgg ctgaacagca
     5161 ctatctgaat ctcaggcctc tcctagaggc ggttggtttc aaggccgtgt tgctcacgag
     5221 cggggggaag gccaaagatc gccaggctgt cttcacgcag ttggcatcgg gggaagcgca
     5281 ggtggccatc ggcacccatg cgctgattca gaagaacgtc gcattcgaca agctggggtt
     5341 ggtggtagtg gacgagcagc ataagtttgg tgtgctgcag cgaaagtcgc tgctcgacaa
     5401 gggatatcga ccggatgtgc tggtgatgac ggccacgccg attccccgca ccttggcgat
     5461 gactgtctat ggcgatctgg atgtttcagt catcgatatg ttgccaccgg gccgcaagcc
     5521 ggtgcggacc atgctctact cggaaggtca gcgccacaaa acttggcagc tggtgagcga
     5581 tgagctgaag gcgggacggc aagcgtatat catctatccg ctggtagaag agtccgagaa
     5641 gattgatctc aaagcggcga tacagggagc ggaacagttg cagcgcgaca tctttccgca
     5701 ggcgcagatc ggcttgttgc acgggaagat gccgtcgtct gaaaaagagc gtacgatggc
     5761 agccttcaag gccggcacaa tacagatttt ggttgcgacg acggtcattg aagtgggggt
     5821 ggatgtgtcc aatgccaccg tcatggtcat cgaacatgcc gaacgattcg gactggccca
     5881 actccatcaa ttgcgcggac gagtaggacg aggggcgcat caatccttgt gtatcctcat
     5941 ggcgtcctat ctcccgcgag agtccagacc gaaggtgagt gctgaaggaa agcctgaggc
     6001 caatgcctcc aatgcccagc aacgcttgtc cgcgctggtg aaatcacacg acgggtttgt
     6061 cattgcggaa gaagatcttc gcatcagggg gcccggcgag ttcttgggat tgcggcaatg
     6121 gggactgccg gagtttcggg cagccaatct cattcgtgat acacacttgt tggagctggc
     6181 gagacaagaa gcgttcgcga tggtaaccca ggatccgggc ctggcgttgc cccagcatca
     6241 ggcggtcaag gcggccatgt ttcgtcgctg gaagggcaag ctgtccttgg gggacgtcag
     6301 ttagttagtt ggcgcaatgg tgctgccgaa ggagcggagg actgatcgat gggaatgctg
     6361 caacgtttga gtcacgatct tcgcgccgga tgggtgacat tacgacatgg cactgcaaag
     6421 gctgccacga gagcattgga agagggcgag cttctggggt atcgattgga attgcgaaag
     6481 gtggagcagc agctggacga tctgcatgtg gacattggtg agcggacgat cgagttgcat
     6541 gaccgcggcg aggcaccgga tcgtattgtg gctgattccg aagtcgcacg tctgatgaaa
     6601 caggtgcagg cgctgcagga tgaacggacc aagctcctat tggaaatgaa tgacatcacg
     6661 gtagaagagg cctgaaagat ttcatggctg gcgcatcaac aggttcgtcc agaatcttgg
     6721 ctggcatcga tatcggaacc ctgacctgcc gcctggagat tgctgaggtg acttcctcag
     6781 gccaggtcaa aaaactgcga tctgatcgac gaattctccg gctcggccag ggggtcgatc
     6841 gagatcgcat gctgcggacc gacgctatgg cgcgggtggt gacgacgctg aaggaatggc
     6901 gccagagtat tgatacatat caggtggagg cgacgtcggt ggttgcgacg agtgccgtgc
     6961 gcgatgccgg gaaccgacaa gactttttag acctgatcgc acgtgaggcc ggcttgcaag
     7021 tggaaatcat ctccggtgag gaggaggcgc gacgaaccac cttggggatt cgttccggtc
     7081 tgccggaagg tgtctctgac atgctggcgg tggatatcgg cgggggaagt acggaatgta
     7141 ttctggatcg acccggccaa ccgctcattg tgcgatccat cgatattggt gtggtgcgcc
     7201 tgagcgaacg cgtgctgcag agcgatccgc ctaccgccga agaacttcag caggcgcatg
     7261 attggatacg cactgagacc ggagcggcca tcgcggatat gcccttgccg gtcggactga
     7321 catttgtggg tacagcggga acgatcaccg cgttggcggc tatggagcag caattgcccg
     7381 tctatgaagc agcgaaaatt cacaactatg ttctgacgct tgaggcggtg aagcgcctgg
     7441 aggcacagct tcttggtcga tcgaaacagc aacgggaggg gttgccgggg ctggagcgag
     7501 gccgggagga tgtcattgtc gccggagcga tcatcctcag tacgatgatg gagacgctgg
     7561 gcatgacgaa ggttctggtc agcgacctgg ggcttcgaga aggggtgctg ctccacctgg
     7621 cgagccggtt gaacggaggt tcctcgttgc gctgagtggc ggcctgacgg tgataggctc
     7681 tcgttggtga agggatgacg tgctgatcgt taccgttggg agtaccagcg cagcccgctt
     7741 ttttgttcgg tgaaggtggc ctgaggcatg ccgcctggtt tttccagaag taagaccttg
     7801 aagtcgatca ggccgaacca ggctggatcg gcaatatcgt ggtaatggca gccctgcagt
     7861 ttcggaaggg tgtcaccgag ggtcttcgtg agctcacttt cggtgtaggc cctcaccgag
     7921 aagtgttggc caagtccctg gcaaaaccgc tgaatcgtat aggctgcgcc ggtgcaaagg
     7981 accttcgtat ccggtttgag ctgtaaaaat tgcgggagcg acaaatagcg ctcctgaaag
     8041 cccagccacc ggaccgcttg ccgcaaatgg ctatactgca cttcgtattc ggtgtggtga
     8101 ggaagtttca ccgcggcagg ccagccttct tcgatcccga actccgtcaa aaatgcccga
     8161 ccgcccggtt tgaggactcg ccagacttcg gcgacgaatc gcatcggtcc cagattgaag
     8221 atcacttctt ccggtagctc tttgtcgagt ggcaaccgca gtcgcctgat ccaatccagt
     8281 gcttcctgat gttgcgcggt ttccccccgg ccactcgtga gttcctcccg gctgagcttg
     8341 accggggtca tgtccgccat gttttcgttg tcgatgatga ggtcgacgga gttatcctta
     8401 aacggcagcc attccccgtt ggcatgagtg ccggtgcctg tccagccacc ggccttggcg
     8461 cgcttggttt gcagttggag gaaggggcgt gtgacgtcca acgagatata ctggatcccc
     8521 tgcttttcaa acggcaacag ctcttggccg agttcgcggg cgacatagcc gagtccgcct
     8581 ccgatttcca cgatcacctt gggtttcggt ttgaaccagc ccaggcggcg aagttgacgc
     8641 atgagcaggc gtccataggt ggtgcctccc agagcctcgc agggttctcg gaagaggtgc
     8701 gaaacggtcg tttcaatgag atcgaaatgg ccgtcctcca gatcgctttc tttcagctcg
     8761 tgaacatgga aatcttcgag atgttcttcg ccttcgaaat cctgttggcc ggaccagctt
     8821 tctcgtattt gctggagcag gaggtcccat ttagccttgt gccggtggcc ccccggcggt
     8881 tcggtcccga aatagcagag tgaatagttg gggatcgacc agtgtgccag atgccaggat
     8941 cctggctgac cgtcaggccc gcagatcttg ttgccgtatt tctccacaag tgcaccgacc
     9001 gtaatgtggc cgttcatgga ggtgagcatt tcaatgccga ctgcgttcag gcggacgagc
     9061 gggaaatcat catccagggg cgccaaccac cattcgtcaa aggcatgctg atacgcaacc
     9121 aggtcgggaa tgcgccaggc gagcaggctc agaatgtcgc cgctcagatt gagcggttct
     9181 tgaatggctg cggcagcttt catcgcgtca gacgattcag attacaagaa gccgcagcct
     9241 acacgaggct cacgctgacc tgcaagaacc caggagggtc gtctctaggt ttgtgagggc
     9301 aggaggggag agtcggtcac gatgatgatg ccgcgcataa tgggatgaat gcggcattga
     9361 tacgggtatc ggcctggagg caggctggga atggtataag tgccacccgg ttccaccgcg
     9421 ccggagtcga aggcgcaacg tccgctttcg tcaaaacagc cgtcgtgcgt cacggtgtga
     9481 tgggtcggcg ttgggttgtc ccaccggatg gcgccacctg cggtgacggt ggcggcagcg
     9541 ggcacgtaat agggggaacc gctatccatc aagacatgga ctgccggagg ggtcgcggag
     9601 gagggccaag ccatcaggaa tgcgaacgtg agacttagag agagtgtgcg ccagctcatg
     9661 aggtacatga ctccatgtga atatcgatct cttcattata catagagaca ggcgcgatgc
     9721 ggaaggattc accagactgc tcctaggctg ggcctctttg aaacagcgca aaccggatga
     9781 ccgaatcgaa ggcaccaaga gtgagtgtag gctgctggaa ggtttggctt ggcgttgcac
     9841 gatgacacaa tttcgatgtg aatcggagag gttgcaagaa cctattggtt catgctagat
     9901 ggaggccgct ttaccaccga cgtgccatgt tgaagacctg agaggaggac gtggaatggc
     9961 gaagaagacc atgaaaaaga aaagctctgc ggtgaaagtc gcgcggccga aaaagggcgc
    10021 ggctttcaag gcgagcaagc cgaagaaagt cgtgaaagcg aagaaggccg cacccaagaa
    10081 gccggtgaag gccaagaagg cggcgcaacc gaaggtgacg gcgaagaagc cggtgaagaa
    10141 gaccgctaaa aagagtactg ccaagactgc tccggcgaaa gccgcgcgtt cctccaaggc
    10201 gaaagcgaca ccaaaagccg ccaaacctgc gccgaaaccc gcatccaagc ccaaaacgaa
    10261 gcctgcttca ccaaaggcga agacgcctgc ggaagccccc gcggcggtcc ctgaatcgaa
    10321 gccggtcaaa tcttccgtgc cggagcctga gaaggcctca aacgtcgaga tggagccgac
    10381 ccagcttgac ctcgaagatg acgaagagga catggctgaa gatctggatg acgtatcaga
    10441 agaagaaatg gaagaagaaa tcgaagagga actcggcctc gacaatgagg acgacgatgt
    10501 cgattatctc gagaagtctg aagatctgct cgacgatgca gatgagtatc gaaacaatta
    10561 gtttcagttg atgccgctgt accgggtgat ctcggtctgt gcctctgcgt gcctcattga
    10621 ttctcgtcag tgaaggtccc actgaacatg tctgatgtca tggtgtgttg cgccgcgtct
    10681 cgaatgaagt cgcgtcgcgc cttccgatcg tgtcgcatcg ttcgggtgcg gctcgcggcg
    10741 acgtctgcca attccaatcg ctcagccctc tcatgagcca ctttcttttt atcgccgggg
    10801 ccttacgcga acgaacttcc ccggaaagtg ccgagttgca gctgggcgcc gggatctggg
    10861 gcctgtgtac ctccttgatt cgcgacaacc ttcaaaagtt cctgacgccc gaatcacatg
    10921 gcctggtgta tgtgttgaaa gaaggcattc gcgcggaatt ttcgattgtc tcgccggtcc
    10981 agccgtttca ggcgttggac gaactgctga aagatgagtt gcggacggaa gcccgctatg
    11041 gattcgtaca ggtcacaccg attcgacgct gggaggggaa cgcgcccgag tgccagcaac
    11101 tgcttcagcg agtcctgggc atcgaagaac catcagaatt gtcccgtcgg ctcagtcttg
    11161 ggatgcaccg tctttcacag gaggaatacc gagcgattgt cgacgggttg ggggcggaac
    11221 actaatcaga tcgcgcttcc ttccggaagc ggctagtcga gggtgccacc tgtcatgaga
    11281 tggtaggcct catccaccgt ggcgacttct ttgatttcta cgttgagatc ccaggcaagg
    11341 cgggtgagat tccagcgggg gtcgtctcgt tgaccttgcg ggatgaggat ggtgcggtat
    11401 ccctcgcggg ctgctgcccg gatcttgtcg gggatgcttc ccaccgggcc gatcgtgccg
    11461 tccttggaaa tcgtgcccgt catggcaatg ccgcggcgga tctgttcgcc cttgaggagt
    11521 gcggcaaatc ccaccgtcat gacgcctccg gcactcggcc cgtcggtacc ggttgcggcg
    11581 tacgaaatgc cgatgacact gatggtcccg gtatggttca ccgtcggtgt atgttcgaga
    11641 gcatattgta aggcgaggac catagaccca aggttcgtct gtccgggttt gacgttcccg
    11701 ccttgccatt gcagcatgat cggagaaggg gtcggctgat gatcccagcg cagcaggagc
    11761 acttcgaaaa cgccaagctg gttggactga atagccgcca acatgggaac ttcgacgact
    11821 gactcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggat tctctgggga
    11941 accgtcatgc ttcccggtct cttgttcgcc gaatgtacga gtgagaccgg tggaaagact
    12001 gccagcgcag tcttcactct gcacttgacc ggcacctgta cggaagcgga gcgggaggct
    12061 cgtgccgttc cggccaaaga tctcatgcga gccttggcgg ccgggaaagg cattgatctg
    12121 gcgggagtgg tcattcaggg cgatctggtg cttgacgagt taccggcgca gaaggttgac
    12181 gcggtgcagg gcctgtccct cgaagatcgc cgggtgctcg aagggctcaa cgatgaagag
    12241 gtgcatgtca ttcgcgggcc attcgtgatc aagcactccc gggtgaaggg gcagatcgtg
    12301 aatcgtctca agcgcgggtt tttgctgatt accggcccgg tggtgttggt gcacagcggt
    12361 ttcgacggcc tcgtcgattt gtcacggaca gtctttctcg gtctggttga tgggtcgaat
    12421 gccaagttcg agaaggaaag ttattttgtt caggaccgct tcacgcaggg cgcgatgttt
    12481 tccgataccc gcttcggatc gcatgcacgg tttcatcggt ctatgtttag cggtccggcc
    12541 atctttcgcg gcgcagattt tcctggcctg actgagtttc tggaagtggt attcgagcag
    12601 gacgcgaatt ttgctcgtac ggccttccat atgggaaccg gattctctgg tgcgcactgt
    12661 cgtggcaagt gcgatttttc atcgacgctg tttgagcgag aggcgttctt tttgtttgct
    12721 cgatttgacc gggcggtgac ttttgcctcg gcgaaattca gttcccaagc cgatttttcc
    12781 gacgcgtcct tcaaggaggc tgacgactta gcaaaggcta cgttcgttcg cccgccggtc
    12841 atgatcagaa ctgcgcgggt cgtttcgact gtgcccgtcg ccccggaagc gggtcctttt
    12901 tcccaagtgg tgacgatcgg gctgtttgtg gcggcgttgg gaattttgat ctatatcctc
    12961 aaagcgaagt agttgcctga accccaacat atagttttag ccttgcccca tcccaccata
    13021 aattggtata agattaaaaa tttgtccaag ggagcgtctg gtacctgatg gaccaacctg
    13081 aactgcccta ccaagtcaag atcgagaatt ttgaaggtcc gctcgatctc ctgttgcatc
    13141 tcatcaagaa aaacgaaatc aacatctacg atattccgat tgcgctgatc gctcagcagt
    13201 atctggacta tctgtcggtg atgaaggaat tgaaccttgc ggtagcgggg gagtttttag
    13261 tgatggcagc cacgttgctg cacatcaagt cgcgtatgtt attgccgacg gacgaagtcg
    13321 ctgtcgacga ggaagacggg cctgatcctc gagaagaact ggttcggcga ctgcttgaat
    13381 ataagcagtt caaggaggcc gcttcgcagc tggattataa ggaacgcctc tggcgggata
    13441 cgtttggccg tgagcccggt ccgccgatcg aaatcgcaaa agatgaaagc ctgctcgacg
    13501 agatttcgct gtttgatctg gtcgatgcat tgcaaggcgt tctgtcccga catcccggaa
    13561 aacgcctggt tgaaatcatc ccggacaacc tgacggtccg tacacgcatg agcgcgatta
    13621 tcgaggcgct tgaggtgcac gaatctatgc cgttcacggc gctgtttgag gagtcgagtc
    13681 atcgcctggt cgttattgtg acattcctgg ctttacttga gctgatccgc atcagggtcg
    13741 tgcgggtttt tcaaagcgaa accttcgggg cgattcttgt ctcccgtgcc tttacggcgg
    13801 tggcagaagg ggatttagtg gaggagtctg aatggaagaa cctatgagtc tcagcatgga
    13861 ggacgccgtc gaacagcctg ccgaggtggc tgaagtggag gcgccagtgt ccgaagagcc
    13921 gactgctgaa cctgaggtag aggcccacgc cggcgaaggt gacgtggcgg agatggctgc
    13981 gccgacacca gcgtctgatc ccgctctttc cgacgtgcga gccttgaagg gaatccttga
    14041 ggccttgctc tttgtgacgc ccgacccaat tcctgtcacg cgattcttgg ccttgcttgg
    14101 ggccgtaaca aaacaagagg ttgatcaggc actggccagt ctgagtcatg actacgaaca
    14161 ggagggccga ggcctccagt tagcggaggt
//
LOCUS       DAOQDS010000087        13866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1965_length_13866_cov_22.221925, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000087 DAOQDS010000000
VERSION     DAOQDS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13866
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1965_length_13866_cov_22.221925"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PK589_16305"
     CDS             complement(<1..508)
                     /locus_tag="PK589_16305"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNJ21225.1"
                     /translation="MEELTDILVGLEQRISAYRNRYQELEKKRRRLDDEIAMIKKYLE
                     LAETLYRVEADKAKLASLSSQIITDEKGIRPLPVTDVTDQSREILLGKSKYVGKSVPE
                     AAYQILREASRPMHAKELLQRLLEGGLQIKGKTPLTSVATSLKRDKRFQKVGPNTFAV
                     TTQQELTAV"
     gene            757..1578
                     /locus_tag="PK589_16310"
     CDS             757..1578
                     /locus_tag="PK589_16310"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNJ21226.1"
                     /translation="MHTSHALPSEELDRYLATAVQAAEAAGHVLLEHAKTGFRIDHKA
                     AINLVTDADRQAEECIVRSILSSHPTHRILAEERGRDGATESPYQWIIDPLDGTTNFA
                     HGFPFYSVSIGLEYHGDCIVGVVLDPTRQELFTGVAGKGAYLNGERLRVSNNETLGRS
                     LLVTGFAYDIRETTNNNLDHFSRISLRAQGVRRTGSAALDLSYVASGRFDGYWEVKLS
                     PWDMAAGIVIVREAGGLVSGFQKDTFSLYGQELVATNGLIHQQLLDVIHQRPATS"
     gene            1706..1975
                     /locus_tag="PK589_16315"
     CDS             1706..1975
                     /locus_tag="PK589_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21227.1"
                     /translation="MASQVPPQKPAAPSANAQDVWDVELLHVHVSRKGQLVNAEWALH
                     PQIKADLSAEEWRELGELMNKVTTIVGHRFSQALNEAEPTPPGNA"
     gene            2062..2394
                     /locus_tag="PK589_16320"
     CDS             2062..2394
                     /locus_tag="PK589_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HNJ21228.1"
                     /translation="MDCYYHSKDLGKFGDMGKGNPVLWEKFMSYYSAVFAEGALTERE
                     KALIALGVAQAVQCPYCIDAYTQACLEKGSNIEEMTEAVHVACAIRGGASLVHGVQMR
                     NVAEKLSM"
     gene            complement(2571..4436)
                     /locus_tag="PK589_16325"
     CDS             complement(2571..4436)
                     /locus_tag="PK589_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HNJ21229.1"
                     /translation="MSNSQAKVALVNMPFSFSKYPSIQLGTLSALLKAKGVEVDCHHL
                     NVRFAHKIGVPLYESICEKRALFGEWLFSYLLFRDNPKRAAYPRVFKPVFEQIARESG
                     QPVSFFEDMGSRTAPQFLTAAMAGIYWGQYKLVGFTSTFDQNVASLTMAKLIKDLYPD
                     VKIVFGGANFDGEMGLEYFRAFPFIDYVVVGEGEVTFPALVDYVLRGSTGPCPKGVIY
                     REDGEVKLEPNTALFTEFAQTGPPDYDDYYHLLAELGTEASRGLDRILLYEGSRGCWW
                     GEKHHCTFCGLNAQSMKFRAKSAEQVAQEMAYLSSRYDTTRFRLVDNIIDMKYVDNLF
                     GRFAAAHCDLDVFIETKSNLQKSQIRTLAVGGVKCMQPGLESLSLSQLKAMDKGVTPM
                     QNIICLKWSCYYRVAVSWNILLGFPGETNEDYRRQIDLLPSLFHLQAPEATGKFWLQR
                     FSPYFTRPHEYGVRITGPGTAYEYVYDAARVDLRKIAYDFEYELDDWPVDPQVYQELT
                     GLIEEWQRRARSNDRPFLYYSKAMEYVTIYDGRDPDMPTRQRYDWPAAGIIEACNEAA
                     KSRDQIRAALGEAKRDSIYSEDDLTEALSLLTSRRILYEEREKYFTLAIPEHPYY"
     gene            complement(4631..4978)
                     /locus_tag="PK589_16330"
     CDS             complement(4631..4978)
                     /locus_tag="PK589_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNJ21230.1"
                     /translation="MSLADNKCIPCRGGVPPLPADRTQALLAELGRGWALSKEGHLER
                     LYTFKDFAQSLAFANKVGAVAEAEGHHPDLYVAWGKCKVEIWTHKINGLTESDFYLAA
                     KADREFEPFRAGA"
     gene            complement(5063..5551)
                     /locus_tag="PK589_16335"
     CDS             complement(5063..5551)
                     /locus_tag="PK589_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ21231.1"
                     /translation="MLVQEVMSTGVVTAKRNESVRSVVTKMLSRHCGAIPVVEDGDVL
                     IGMVTLRDVLIPLYPNYGEYIHDNVHSRDFVEMEEGYADVLSQRVEEVMSLNPLTVSP
                     RTPVLEAASYMGVKNFRRIPVVDKGTLVGMVSVGDINRGLFFERGHAVTSQYRVAQPS
                     GR"
     gene            5881..>6476
                     /locus_tag="PK589_16340"
     CDS             5881..>6476
                     /locus_tag="PK589_16340"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNJ21232.1"
                     /translation="MWKARSFHADFVEIDLRETSDGHLVLLHDDTIDRTTNKSGNLAE
                     MSLEQVQRLDAGNWQRIPTLEEALDISGKAIGVILELKVEGIGNEACAIVKRVGFSNS
                     LIYASFLIPELHRVRQADPAAQIMVLLHRHLPLDPVADVVALNASHVGLHYSTVTPAL
                     LQTYHDLGRQVFVYTVNDWTEIRRMRELGVDGIVSDFP"
     assembly_gap    6477..6576
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8010..8888)
                     /locus_tag="PK589_16345"
     CDS             complement(8010..8888)
                     /locus_tag="PK589_16345"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNJ21233.1"
                     /translation="MRMKAAFLIGLVSSLTALPAYAGDFEGVIHMKNSHVDTGTSRAM
                     DWYLKGDRGRVETIREDGQAHVMLFNGKTRTMQMGMPGQKSYMEISLEGDRGEHLNEL
                     LEAQHVERTGKTEKIAGYTCEVWRITDKEQHRPKSELCVAKGFGKAATFWIDPKEARR
                     SSQPGWVKQLAEEGGFGLRSIQYDEAGKESGRMEVVSIEKKTLDASLFAFPADWAKHD
                     LSGFQERMKAMREQKREGGADVSKMVEEMKKRKAARGKPGEAPGGEEQPPDMKELMKQ
                     FGEAMKKQQSQPQGGQ"
     gene            complement(8985..9803)
                     /locus_tag="PK589_16350"
     CDS             complement(8985..9803)
                     /locus_tag="PK589_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNJ21234.1"
                     /translation="MERYRIKPGCRPSLKRFDPDDTGSYKKNAAGKAKAKTAAAKHIA
                     TLDQLQERLYANRTRALLIILQGMDTSGKDGTIKHVMSGVNPQGCHVASFKAPSEKEL
                     EQDFLWRIHQEVPAKGYIGIFNRSQYEDVLITRVHGLVSKKIVTQRFNQINEFEELLV
                     ENGTVIIKLFLHISKEEQKARLTERICDPEKRWKFSEGDLEERKLWDDYMTAFEDLMA
                     ATSTERAPWYIVPANRKWYRNLVVAELVMQALAAMKLSTPPAPKGVNFDGLTID"
     gene            9958..10443
                     /locus_tag="PK589_16355"
     CDS             9958..10443
                     /locus_tag="PK589_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNJ21235.1"
                     /translation="MLKEVSGDILLSTAGAIAHGIAPHDNFKQGLALALREQWPGMYK
                     DFRHYCQTYNPKPGGAWSWKGPNSPVIINLFTQEPPATEQDHPGKATLPNVNHALQAL
                     KKELQEHQVKSLALPRLATGVGGLEWEKVYPLLQQALKELHIPVYVYGLYKKGVAAQE
                     A"
     assembly_gap    10836..10935
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10936..11624)
                     /gene="otsB"
                     /locus_tag="PK589_16360"
     CDS             complement(<10936..11624)
                     /gene="otsB"
                     /locus_tag="PK589_16360"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNJ21236.1"
                     /translation="MFSAQGKRVLDRLASQGGSLFAFDFDGTLARIVQDRHAAGLTES
                     TREALQGLAEVAPTAIISGRSLADLAPRVKGIAAHLIGNHGLEGLHASERVMQQAQDC
                     CSAWLKAVTKDALDLTRAGVVVEDKMYSLTFHYRQAWSPAAARETIFHVVSTLLPAPR
                     LVLGKAVVNAIPSGNLHKGTAMLELMHQLQISAALYVGDDDTDEDVFSLPDERIVSVR
                     VGKKAATAAQW"
     gene            complement(11642..>13866)
                     /locus_tag="PK589_16365"
     CDS             complement(11642..>13866)
                     /locus_tag="PK589_16365"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNJ21237.1"
                     /translation="SLRFILPLLLVLGVVAYSVVPLVDSLTLKWFTRDLESRSKLLAS
                     TMEVPLADLVVSRSRTKIFAYFHKVIQDERLYALGFCDTQQRLLYQTLTYPDQLSCES
                     LAHLAPGSSEVVDFAQGPLHVVVATIQSGGKVQGRLMLVHDMSFVHQRSTDTKWYIFY
                     LFVGLAGVISAVTVLVAQLSWRGWVAGVRAMLTSKGLPAESSQAHSAELHPVAQDLHA
                     LVRELEADRRMRDESQITWSPASLKTILHEHLSGDEILIVSNRQPYIHNRRGQKVEVQ
                     VPASGLVSALEPVMRACSGVWIAHGNGSADKEVVDNRDHVRVPPDHPSYDIRRIWLSP
                     DEESGFYYGFSNEGLWPLCHNAHVRPTFRTSDWEQYVAVNERFAKAVVEEAKTDDPVI
                     LVQDYHFALLPKMVREKLPNATIIMFWHIPWPNSESYGICPWRQEILEGLLGSSIVGF
                     HTREHGNHFLSCIDRILEARIDRDSSTVSYGGRLTAVNPYPISIEWPSRWLENQRPVP
                     DCRVHIRERHAMGPDRLVGIGVDRLDYTKGIIERFLAVERLFELQPEWVGKFSFIQIA
                     APSRTIIDQYQHFHDQVYALAERINKRFGREGYEPIVLKVKHHEPPDVYEYYRASDLC
                     FVTSLHDGMNLVAKEFIAARDDEQGVLILSQFTGAAQELPEALVVNPYDIDQCAAALH
                     MALSMTPKEQRARMRSMRGLIQEFNVYRWAGRMLMDAARMRRRIRVMKQVGQASGRGR
                     "
BASE COUNT         2950 a         4141 c         3691 g         2884 t
ORIGIN      
        1 ctaccgctgt caattcctgc tgcgtcgtga ccgcaaacgt attgggtccg actttctgga
       61 aacgcttatc ccgtttcaac gaggtggcga cggaggttaa tggcgtcttg cccttgatct
      121 gcaatccacc ctccaaaagc cgttgcaaca gctccttcgc atgcatcggc cggctcgcct
      181 ctcgaagtat ttgatacgca gcctccggca cactcttccc gacatacttg ctttttccaa
      241 gcaagatctc gcgcgactgg tccgtgacat ccgtaacagg aagcgggcga atcccttttt
      301 cgtcggtgat aatctgactc gagaggctgg ccaacttggc cttatctgct tccacacggt
      361 agagcgtttc ggcgagttcc agatacttct tgatcatcgc gatttcatcg tcgagccgac
      421 gtcgcttttt ctcaagttcc tggtagcgat tccggtaggc gctgatccgt tgctccagac
      481 ccaccaggat gtcggtcaat tcttccacaa gcaagtcctc aataaagcat acttgcttta
      541 tagcaagtga ttcaaaatct gtcaagtaac tgatcataac tatttgttcg cgcttatcgc
      601 ctatgcaagc agaatgcact cagtgcctag ccaagccttg ttctgcaaga acaagctttt
      661 attaaatcat ttattttcaa tatgttaatt tgctctctca aaatgaccaa ggctttttac
      721 cccgctttac accactcatg ctaggatacg gcgtctatgc atacgtcgca tgcactccca
      781 tctgaagaat tggaccgcta tctggccacg gctgttcagg cggcagaagc agccggccac
      841 gtgctccttg agcatgccaa aacagggttc agaattgacc acaaagccgc catcaacctc
      901 gtgaccgatg ccgaccggca ggctgaggaa tgtattgtcc gttcaattct ttcgtctcat
      961 cccacccacc ggatcttagc cgaggaacgc ggccgggacg gggccaccga gtcgccatat
     1021 caatggatca tcgatccgct cgacggcacg accaacttcg cgcacgggtt cccgttttat
     1081 tcggtctcga tcgggctgga atatcatggc gactgtatcg taggcgtcgt cctggacccg
     1141 actcgtcagg aactgttcac gggtgtcgcc ggcaaagggg cctacctcaa tggcgaacgc
     1201 ctccgcgtgt cgaacaatga gaccctcggt cgttctcttc tcgtgactgg atttgcgtac
     1261 gatattcgcg agacgacgaa taacaatctg gaccatttct cccgcatctc gctgcgcgct
     1321 caaggcgtgc gacgcaccgg ctcagcggcc ctggatctca gttatgtggc ctctgggcgg
     1381 tttgacggat attgggaagt gaaactcagc ccttgggata tggccgccgg gatcgtcatc
     1441 gtgcgtgaag cgggcggact ggtatccggc tttcagaaag atacattttc tctctatggg
     1501 caggagctcg tggccaccaa cggcctcatt catcaacaac tgctcgacgt catccatcaa
     1561 cgacccgcca cgtcctaacc tcctgagcgg tgatatccga cccggccgat gcgattggca
     1621 ttcacgtcgc tcgtcccatg tacagtgcga tatttgatgc ggtatcatgc cctctcatct
     1681 gtatggtcaa aggagtgcac gcagcatggc aagccaagtt cctccgcaga aaccagccgc
     1741 cccgtccgcc aacgcccagg atgtgtggga tgttgagctc ttgcatgtgc atgtctcgcg
     1801 taaggggcaa ctcgtgaatg ccgaatgggc ccttcatccc cagatcaaag ccgatttgag
     1861 tgcggaggag tggagagaac tcggcgaatt gatgaacaag gtcacgacca tcgtgggaca
     1921 ccgtttctcc caggccctca atgaggccga accgacacct ccaggaaacg cgtaacgctt
     1981 ctcgccatca cagaccgcta ctacgcatgc tcatcggctg acgcgcgagc atgatcagac
     2041 cactaaccag cgaggtgatc cgtggactgc tactaccact cgaaggatct tgggaaattc
     2101 ggagatatgg gtaagggcaa tcccgtcctg tgggaaaagt tcatgagcta ttacagcgcc
     2161 gtatttgccg aaggcgccct cacggaacgg gagaaggcac tgattgcact tggtgtggcg
     2221 caagccgtgc agtgtccgta ctgcatcgat gcctatactc aagcctgcct ggaaaaagga
     2281 tcgaacatcg aagagatgac ggaagccgtc cacgttgcct gcgcgatccg cggcggtgcg
     2341 tcactcgtgc atggcgtcca aatgcgcaat gtggcggaga agctgtcgat gtgatgtttc
     2401 agcggcgacg cgtttgcagc agaaacgtgc cagccaatgg gacgagtgga tggtcaaaga
     2461 ggaggcctag caaggccgca gcacgtgcag gaccgagacg aaggagacct gtaccttgag
     2521 gtactgagct taggcaagaa acaacgccgg ctttctgcct caccagccca tcagtaatag
     2581 ggatgctcgg ggatagccag cgtgaaatat ttctcgcgct cttcgtaaag gatgcgacgg
     2641 ctggttaaga ggctcagcgc ttcagtcaga tcgtcttccg agtagatgct gtcccgcttc
     2701 gcctcgccca atgcggcgcg aatctgatcg cgactcttcg cggcttcatt acatgcctca
     2761 ataatccccg ccgctggcca gtcgtatcgt tgtctcgtcg gcatgtccgg atcacgcccg
     2821 tcgtagatgg tcacatactc catggccttc gaataataca agaacggccg gtcgttgctg
     2881 cgagcccggc gctgccattc ttctatcagt ccggtcaatt cctgatagac ctgcggatcc
     2941 accggccagt cgtcgagttc atattcaaaa tcgtaggcga tcttccgcaa atcgacgcgc
     3001 gccgcatcat acacatattc ataggccgtc cccggacccg tgatgcgcac tccgtactca
     3061 tgcggtcgcg taaagtaggg gctgaagcgc tgcagccaga atttcccggt ggcctccggc
     3121 gcttgcaaat ggaataggga gggcaggaga tcaatctgcc ggcgataatc ctcattcgtt
     3181 tcgccgggaa acccgagcag aatattccag gacaccgcga cccgataata acaactccac
     3241 ttgaggcaga tgatgttctg catgggcgtg acgcccttgt ccatcgcttt cagctgcgaa
     3301 agactcaggc tttccaggcc gggctgcatg cacttcacgc cacccacggc cagggtgcgg
     3361 atctggctct tctgcagatt gctcttggtc tcgataaaca cgtccagatc gcaatgcgcg
     3421 gcagcaaatc gtccaaacag gttgtcgaca tatttcatgt cgatgatgtt atccaccagc
     3481 cggaagcggg tcgtgtcgta gcggctcgag aggtacgcca tctcttgcgc gacctgttcg
     3541 gcggatttcg cccggaattt catgctctgc gcattcagcc cgcaaaaggt gcaatggtgt
     3601 ttctcgcccc accagcagcc tcgggaacct tcatatagaa gaatacgatc caggccgcgc
     3661 gaggcttcag tgccgagttc cgccagcagg tggtagtagt cgtcatagtc cggcggtccg
     3721 gtctgggcaa actccgtgaa cagggccgta ttcggctcaa gtttcacctc accgtcttcc
     3781 cggtagatga cgcctttcgg gcaggggccg gtcgacccac gcaagacata gtcgactaat
     3841 gcagggaacg tgacctctcc ttcgccgacc accacatagt cgatgaacgg aaatgctcga
     3901 aaatattcca gccccatctc cccatcaaaa ttcgccccac cgaacacgat tttcacgtcg
     3961 ggatacaagt ccttgatgag cttggccatc gtgaggctgg cgacattttg atcaaaggtg
     4021 gacgtgaatc cgacgagctt gtactgaccc caataaatcc ccgccatcgc agcggtgagg
     4081 aactgcgggg ccgtacgaga acccatgtct tcaaagaacg acaccggctg accgctctct
     4141 cgcgcaatct gctcgaacac cggcttgaac acgcgcggat acgcggcccg cttgggattg
     4201 tcacgaaaga gcaggtagga gaacagccat tcgccgaaca aggcccgctt ttcacaaatt
     4261 gattcgtaga gaggaacacc gatcttgtgg gcaaaccgca cattgagatg atgacaatcg
     4321 acctcgacac ccttggcttt caacaacgca gaaagcgtcc ccaattgaat ggagggatac
     4381 ttggagaagc tgaagggcat gttgacgagt gccactttcg cctgtgagtt actcatacga
     4441 tccccagtcc agccggcata aggcgggggt caaacccgga taagtcatgg acatcgtcgc
     4501 tcgccgcggg tgcccgcaga gaggacgacg caggcgcatt caccgcaatc cgtcgaggag
     4561 gcagagcgag agtgcccgcg ccgcccaaga ggatcggctg tcgcagtaga tgcgccatga
     4621 tttctcccgg ttaggccccc gcccgaaagg gttcgaactc acgatcggcc ttggcggcca
     4681 gataaaaatc actctcggtg aggccgttga tcttgtgcgt ccagatctcc accttgcatt
     4741 ttccccaggc cacatacagg tccgggtggt gcccttcggc ctctgccact gctcccacct
     4801 tattggcaaa tgcgagggac tgcgcaaaat ccttgaacgt gtacagccgt tcgagatgac
     4861 cttctttact caacgcccac cctcgcccca actcggccag caaagcctgt gtacgatcgg
     4921 cgggaagggg aggcaccccg ccccggcagg gaatacattt attatccgct aaactcatgt
     4981 cgctccttcg caaataaatc tcggagtcgg tgtttgccgg aaagtccatt ctacgagccg
     5041 ctcgtcgtat cccgcaaccg ccttaccgcc ctgatggctg tgcaacccga tactggctcg
     5101 tcaccgcatg tcctcgttcg aaaaacagac cgcgattgat atccccgaca ctgaccatgc
     5161 ccaccaacgt gcctttgtcg acgaccggga tccggcgaaa gtttttgacg cccatatagg
     5221 acgcggcctc cagcaccggc gtgcgtggag acaccgtcag cggattcaag ctcatcacct
     5281 cttcgacacg ctgcgagagg acatcggcat acccttcctc catttccacg aaatcacggc
     5341 tgtgcacatt gtcgtgaatg tactccccgt agttgggata gaggggaatc aacacatcgc
     5401 gcaacgtcac catgccgatc aacacgtcgc cgtcctccac gactggaatg gcgccgcaat
     5461 ggcggctgag catcttcgtc accacggaac ggacggattc attgcgcttg gccgtcacca
     5521 caccggtgga catgacttcc tgaacgagca tggcaacctc ctttttcagc agggtgacct
     5581 gtgagtccat caccgaaagc acgattcggt cgccgtcttg ggcagctcaa tgccacttcg
     5641 ctcctgacga ggacggttcc tcgacaccgc cacacatccc gtccgcgtca actgtccttt
     5701 gcagagatat gcaaaatatg gtagggaagt gcggttagaa aagaaacccc ctctcgccgt
     5761 cgccacatat gagcggggag cacccttcag accttctcac gcgaggtcgc tatcgattcc
     5821 gtcctgcgca tcggtcatcg cggagccgcc ggccacgctc ccgaaaatac tctcgccgct
     5881 atttggaaag ctcgatcctt tcacgcagat ttcgtggaga tcgacctgcg cgaaaccagc
     5941 gacggtcatc tcgtattgct gcatgacgac accattgatc gcaccaccaa caaatccggc
     6001 aacctcgccg aaatgtcgtt ggagcaggtg caacggctgg atgcgggcaa ttggcaacgt
     6061 atccccacat tagaagaagc gctggatatc tccggaaagg ccatcggagt gatcctcgaa
     6121 ctgaaggtgg agggcatcgg caacgaggcc tgcgcgattg tgaaacgtgt gggcttttcc
     6181 aattcgctca tctatgcctc gttcttgatt cctgaactcc atcgcgtgcg acaagccgat
     6241 cccgccgcac agatcatggt gttactacat cgccacctcc cgctcgaccc tgtggccgat
     6301 gtggtggccc tcaacgcctc ccacgtcgga ttgcactatt cgacggtcac gcccgcactg
     6361 cttcagacct accacgactt gggccgacaa gtattcgtct ataccgtgaa cgattggacg
     6421 gagatccggc gcatgcggga attgggcgta gacggcattg tctccgactt tccggannnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagat ccggcgcatg cgggaattgg
     6601 gcgtagacgg cattgtctcc gactttccgg atcgcatctg acctcacggc cggccagctc
     6661 tcacaatcag gcagagcacg cactctgctt tgctgccgat caacaccgag cggacctcac
     6721 gagacggcac ctcgaacgcg aggatgcgca aacacctttt gggcaaaacg ccgatggagc
     6781 atgatccgac ttggccaggc atacaagatg gggcggaggc agagaaacac cgtcaaagaa
     6841 tgagatctgg tcaggcggga ggccgaagga aggggagagg ggcaagactg tcaaaagcaa
     6901 tccggcttaa aatgccacgg gcgaccctac actgcggtgg gaggtcgccc gtggttggcg
     6961 cttgggacgc ctacagtcaa ataggttgtc cgacagacca gacccgttcg agaagatgga
     7021 tgatcaggat gttcgtcggg ggaagcatgt tcccaggatt ctggtgtgcc tcgtgcttgg
     7081 cttcaatcct tcatctttgc gctggctgtt atccttaaac ggatagtgcc atcagacaca
     7141 agccagggaa ggatcggtct gcacgaccga cgatcagccc gccctcaact cggcgggccg
     7201 ttttagtccg gcggcgtaag ttcttccatg cgtggacctc cccggttgag gagtggcgag
     7261 gtctcccgaa caggcgcgca ctgtccatcg cctcgtcacg gttaacacgg cccttgaagc
     7321 ttacaatttc cctcttacgc tcgttagaaa actctgtcaa caggaaaatc ggccctagtc
     7381 atttttcctc atgttcccgg tggccgggag agtcccgata caacacgggc tcccgtgtgt
     7441 gacgggagcc cgtgtgtggc gcttaggacg ccgatcgctc tgtctatgta gcggcccgta
     7501 gaagacccgc cgagttgatc aatagcagaa gccatctcgc cgatgagctc gcgatcacgg
     7561 ccggcctgat gaccgcgtca aagatcgtca acctcgccct ccattcggtg gcatgacgcc
     7621 gggacgcgca tgagcgcgtc gtggaatgcc ttgtggcatt tgcgatgaga accggttggc
     7681 aaaaaaaacc gacgagaaac ctgccttcag ttcggcaggc cgtacaggcc aggtggagag
     7741 gtatcggtca tcgtctaccc tcccttctgt tgcgggaatg tgtggaagat ctagatggca
     7801 gccgcgcggt ctgtccggtc ttccacttgc gacaagcgaa atgggccgca gcccctatct
     7861 acgcccctca aaaaaacttg tcaacaaggg gaattcccag gtccaccgcc atcggagctc
     7921 acgccgaatt cctgagccgg ccatagacgg atcggattca cctcgctgtg cgatttgatg
     7981 tgccactctt ttcgacccag gcatgccgct tattgcccgc cctgcggttg cgactgctgt
     8041 ttcttcatcg cttcgccgaa ttgcttcatc agctctttca tatccggagg ctgctcctcg
     8101 ccgccaggag cttcgccggg tttcccacgt gcagcctttc gcttcttcat ctcctcgacc
     8161 atcttcgaga cgtctgcccc gccttctctt ttctgctccc gcatggcctt catccgttct
     8221 tgaaagccgg agaggtcgtg cttggcccag tcggcgggaa acgcaaataa gctcgcgtcc
     8281 aaggttttct tctcaatact gaccacttcc atgcggccgg attccttgcc cgcttcatcg
     8341 tactggatac tccgaagccc gaaccctcct tcttcagcca actgcttcac ccagccaggc
     8401 tgagaggacc gccgggcctc cttggggtcg atccaaaacg tcgcggcctt tccgaaacct
     8461 tttgccacac agagctcact cttcgggcga tgttgttcct tgtcggtaat gcgccacacc
     8521 tcgcaggtat atccggcgat cttctcggtc ttgccggtgc gctcgacatg ttgcgcttcg
     8581 agcaattcgt tcagatgttc accccgatct ccctccaggc tgatctccat ataagacttc
     8641 tgccccggca tgcccatctg catcgtcctg gtcttgccat taaacagcat gacatgggcc
     8701 tggccatcct ccctgatcgt ttccacacgc cccctatcgc ccttgagata ccaatccatg
     8761 gcgcgcgaag tgccggtatc cacatgactg tttttcatat ggatcacacc ctcgaaatca
     8821 cccgcgtagg cggggagggc agtgaggctc gagacgaggc cgatcaaaaa ggccgctttc
     8881 attcgcatac tccacgctcc cggttaaggt agacacacga ttcaggcttg ccagtgtagc
     8941 cgagccccac acacgatgcc agatgactct ggcgcgtacg gccattagtc gatcgtgagc
     9001 ccatcgaagt tcacgccttt gggggccgga ggcgtggaaa gtttcatcgc ggcaagcgcc
     9061 tgcatcacga gctcggcgac gaccagattc cgataccatt tccgatttgc aggcacgatg
     9121 taccagggag cccgctcggt gctggtcgcc gccatgaggt cttcaaatgc tgtcatgtag
     9181 tcgtcccaca gcttccgctc ttccaaatct ccctcgctga atttccagcg cttctcggga
     9241 tcacagatgc gttcggtgag acgcgctttc tgctcctctt tggaaatgtg caggaacagc
     9301 ttgatgatca ccgtgccgtt ctcgaccagt agctcttcga actcattgat ctgattaaat
     9361 cgctgcgtga cgatcttctt cgacaccaga ccatgcacac gcgtgatcaa cacgtcttca
     9421 tactgcgacc ggttaaaaat gccaatgtag cccttggctg gcacttcttg atgaatccgc
     9481 cacaaaaaat cctgctcgag ctctttctcg ctcggcgctt taaaggaagc cacgtgacag
     9541 ccctgcgggt tcacaccgga catgacatgc ttgatcgtgc cgtcctttcc gctcgtatcc
     9601 atgccttgca gaatgatcag caacgcccgt gtgcggttgg cgtacagccg ttcctgcagt
     9661 tgatcgagcg tggcaatgtg tttggccgcg gcggttttgg ccttggcctt cccagccgcg
     9721 ttctttttgt acgacccggt atcgtcagga tcaaaccgtt tgagtgatgg acggcaaccg
     9781 ggcttgattc gatacctctc catgtcgttc ctcctgctat ggcagcgtcc tgtggtcggc
     9841 gatcaaagta gtcgccctta tagcgtggaa aaatttattt ctatgaatcc attgattgaa
     9901 gagggcacag tcttatgtga taaacagatc ttttactcaa tcaaggaggc atcggtcatg
     9961 ctcaaggaag taagcggaga cattctgtta tccaccgccg gcgcgatcgc gcatggtatc
    10021 gctccccacg acaatttcaa gcagggtttg gcgttggcac ttcgcgagca gtggccgggc
    10081 atgtacaaag attttcgcca ttactgccag acctacaatc ccaaaccggg cggcgcctgg
    10141 tcgtggaagg gcccgaactc tcccgtcatc atcaatttat ttacgcagga gccgccggcc
    10201 accgaacagg atcatcctgg caaggcgaca ctccccaacg tcaatcatgc gctgcaggcg
    10261 ctgaaaaagg aactgcagga gcatcaggtg aagagcctgg cgctccctcg tctggccacg
    10321 ggagtggggg ggttggaatg ggaaaaggtg tatcccttgc tccagcaggc cctgaaagag
    10381 ctccacattc ccgtctacgt gtatggactg tacaagaaag gggtcgccgc gcaagaagcc
    10441 tagcggccta cggcacaacc gtttttgcat gagagcacat gagcaaccca agtgccttgc
    10501 tggcgtcggc acctcccgca ggatgcgcaa aagatcgtcc agcaaggccg cagcgaagga
    10561 agaggcgagt cgtacccctg ccgtacggtg agtctcggag ggtcacgacg cgctgataca
    10621 gcgcgtcacg cttgtaaacg ccgccgagaa agtgaggcgg cagtgtcctg cgagaacgcc
    10681 gcgcgagggc ctttccgcat cctgttaggc tgagaccgcc ctggcgcggg cgcctgtgag
    10741 atattgcaac aactgcccga tctgcgactg ccgcgtgagg taccattgcg cggcggtcgc
    10801 cgccttcttc cccacacgca ccgagacaat gcgctnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnntacca ttgcgcggcg gtcgccgcct tcttccccac acgcaccgag
    10981 acaatgcgct cgtccggcag cgaaaacaca tcctcatccg tatcgtcgtc tccgacataa
    11041 agcgcggcgg atatctggag ctgatgcatc aactccagca tggctgtccc cttgtgcaga
    11101 ttgcccgagg gaatagcgtt gaccaccgct tttcccaaga ccagtcgggg cgcaggaaga
    11161 agcgtggaca cgacatgaaa aatggtttca cgggcggcgg ctggcgacca tgcctgacga
    11221 tagtgaaagg tcagggagta catcttatct tccaccacga cgccggctcg cgtcaggtca
    11281 agggcatcct tggtcacggc cttcaaccat gcgctgcaac agtcctgcgc ctgctgcatg
    11341 acacgctccg acgcgtgcaa cccctccagt ccatgattgc cgatgagatg ggcagcaatg
    11401 cccttcacac ggggagcgag gtccgccagg gaacgtccgg aaatgatcgc ggtcggcgcg
    11461 acctcggcca gaccttgcaa ggcttcacgc gtcgattcgg tcagacctgc ggcatgccga
    11521 tcctgaacga tgcgcgccag cgtgccgtcg aaatcaaagg caaaaagcga gccgccctgg
    11581 ctggccagcc ggtcgagcac acgtttgcct tgagcactga acacgtcctg catcggcgtg
    11641 actatcgccc ccgccccgag gcctgcccca cctgcttcat gacgcgaatg cgacggcgca
    11701 ttctggccgc atccatgagc atgcggcccg cccatcgata gacattaaat tcctgaatca
    11761 acccgcgcat gctccgcata cgtgcacgct gttctttcgg cgtcatcgac agtgccatgt
    11821 gcaaggccgc agcgcattgg tcgatgtcgt agggattgac caccagcgct tcgggcaact
    11881 cctgggcggc gccggtaaac tggctgagga tgaggacgcc ctgttcgtcg tcacgagcgg
    11941 cgatgaattc tttggccacc aggttcatgc cgtcatgcaa actggtcacg aagcacaggt
    12001 cggacgcgcg atagtactca tacacatccg gcggctcgtg atgcttcacc ttcagcacga
    12061 tcggctcata cccttcacgg ccaaaccgtt tgttgatgcg ttccgccagg gcatagacct
    12121 gatcgtgaaa atgttgatac tgatcgatga tcgtccggct cggcgccgca atctgaatga
    12181 acgaaaactt gccgacccat tccggctgca gttcaaacaa ccgttcgacc gcgagaaatc
    12241 gttcgatgat gcccttcgtg taatccaagc ggtcgacacc gatgccgacc agccgatcag
    12301 gccccatggc atgtcgctca cgaatgtgca cccgacagtc gggaacgggg cgttggtttt
    12361 ccagccagcg cgacggccac tcaatggaaa tggggtaggg gttcaccgct gtcagccgcc
    12421 caccatacga cacggtggac gagtcgcgat cgatgcgtgc ctctaaaatg cgatcgatac
    12481 aggacaggaa atggttcccg tgctcgcggg tatgaaaccc gacgatgctg ctgcccagta
    12541 atccctccaa aatttcctgt cgccaggggc aaatgccgta actctcggag ttgggccagg
    12601 gaatatgcca gaacatgatg atcgtggcgt tcggcaactt ctcccgtacc atcttgggca
    12661 ataacgcaaa gtgatagtcc tgcaccagga tgacgggatc atcggtcttg gcctcttcga
    12721 ccacggcctt tgcgaaccgt tcgttgaccg cgacatattg ctcccagtca gaagtcctga
    12781 acgtaggccg cacatgcgca ttatggcaca gcggccaaag cccctcgttg gagaaaccat
    12841 agtaaaagcc ggactcttca tccggcgaca gccaaatccg acgaatatcg tacgagggat
    12901 gatccggcgg cacgcgaaca tgatcccgat tatccaccac ttctttgtcg gccgatccat
    12961 tgccgtgagc aatccatacg cccgagcagg ctcgcatcac cggctcgagg gctgagacca
    13021 gtccactggc cggcacctgg acttcgactt tctgtccgcg ccggttatgg atgtacggct
    13081 gtcgattcga cacgatgaga atttcatcac ccgacagatg ctcatggaga atcgtcttga
    13141 ggctggccgg gctccaggta atctgacttt catcccgcat acggcgatcg gcttccagtt
    13201 ccctcaccaa cgcatggaga tcctgcgcaa ccgggtgcaa ctccgcggaa tgggcctggc
    13261 tggattccgc cggcagccct ttgctggtca acatcgcccg caccccggcc acccatccgc
    13321 gccaactcag ttgcgccacc agcacggtga cggccgaaat cacccccgcg aggccgacga
    13381 acaaataaaa gatgtaccac ttggtgtcgg tgctgcgctg gtgcacaaaa ctcatgtcat
    13441 ggaccagcat caaccggccc tgcaccttgc cgcccgattg aatcgtcgcg acgaccacgt
    13501 gcagcggccc ttgggcaaag tccacgactt cagaagaacc gggcgccagg tgcgctaagc
    13561 tttcgcagga caattgatcc gggtaggtca gggtctggta gagcagacgc tgctgggtat
    13621 cgcagaaacc gagcgcgtac agccgttcgt cctgaatcac cttgtggaaa taggcaaaaa
    13681 tcttggtgcg agagcgggag acgacgaggt ccgccagtgg cacttccatg gtgctggcca
    13741 acaacttcga ccggctttcc aggtcacgcg tgaaccactt caatgtaagg gaatcaacta
    13801 agggcaccac gctgtacgcg acgacaccaa gcaccagcaa aaggggaagg ataaaccgga
    13861 gggaca
//
LOCUS       DAOQDS010000088        13776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_1993_length_13776_cov_21.439618, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000088 DAOQDS010000000
VERSION     DAOQDS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13776
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1993_length_13776_cov_21.439618"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PK589_16370"
     CDS             complement(<1..299)
                     /locus_tag="PK589_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNJ21238.1"
                     /translation="MLIGVPKEVKDFEYRVSVTPDGVRLLRQHGHQVLVEPSAGQGSG
                     FSDEAYRQAGADVVGSKEEVFKRAELIVKVKEPQLAECALFRPGQVLFTYLHLAS"
     gene            467..835
                     /locus_tag="PK589_16375"
     CDS             467..835
                     /locus_tag="PK589_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21239.1"
                     /translation="MDAGSCNACNTAGTPLMKLSLGKDFFGRTYDRLSPSSDQSPKWY
                     CEPCSMMKNLQRDFRDIRTEFDKLATGQASALSEPEAKQRAQLRLREISAIAGSQTAG
                     SSLLNPADVKQLIDQFHARA"
     gene            883..1233
                     /locus_tag="PK589_16380"
     CDS             883..1233
                     /locus_tag="PK589_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNJ21240.1"
                     /translation="MTGYQRHIFVCTNKREPDDPRGSCSKLGSESLHACFKQEAKRLN
                     LKGIVRANKAGCLDYCAQGPSVVVYPEGIWYRVRTEADVKEIMERHIVNGEPVERLLM
                     PDQARPSLLSPLKP"
     gene            1297..1680
                     /locus_tag="PK589_16385"
     CDS             1297..1680
                     /locus_tag="PK589_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21241.1"
                     /translation="MEKVAFMKAFPGLLSTWDQLKGKTVEAVLPLKSKTGSSAIICTD
                     GSFVVVPLLSPEPYELGEALQAGREYLEPKHPEAYVGYDRLRKKDQDAQRTARLENIL
                     GAIQNNVEQIPELKDRLAQLVKEWK"
     gene            1677..1973
                     /locus_tag="PK589_16390"
     CDS             1677..1973
                     /locus_tag="PK589_16390"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21242.1"
                     /translation="MSSLPQKNMFEIFSQGLFEGVKPMLVIRDHLVRHPDRCTHQAIC
                     VPICPTSAWLSTPPYKFDPSRCLESCRLCLDACPSQAIYAVFRKGEKLLEPQKK"
     gene            complement(1998..2999)
                     /locus_tag="PK589_16395"
     CDS             complement(1998..2999)
                     /locus_tag="PK589_16395"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNJ21243.1"
                     /translation="MLKQVSRSFYLTLNVLPSDVRDEMGLAYLLARAADTIADTELIG
                     RAERLRYLTMFRAQFAGDEVQPQAVQAIQTGLLPHQTDSAERVLLERLPECLALYRQF
                     EQGDRDRIRWLMEVLPNGMEMDLARFPGSSAQDLTALERPEELDRYTYFVAGCVGEFW
                     TRMVCAHRPAMRRWDVDRMSAIGVRFGKGLQLTNIVKDVARDLHNGRCYVPTQWLDEV
                     GLKPTDLLKPESLPTFRPILLRMIRQAVEHLDQGWLYTMALPRLEIRQRLACMWPILL
                     AGETLKRVATAPDLLDPSVNVKAPRSVVYRVMALTTVTGGCGYVGTAYWGRLRKQIM"
     gene            complement(3051..3497)
                     /locus_tag="PK589_16400"
     CDS             complement(3051..3497)
                     /locus_tag="PK589_16400"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNJ21244.1"
                     /translation="MCIGLLPLVLGMLGTGGLGVVAGPAAVLGAEQALQVPPLRVPVD
                     QADGMQRATVILDSYSYTPQHLIVQAGKPVELLLTSITTITPHNFVLRDEAAGLSIER
                     DVSAGKTVTVQFTPTKPGRFAFYCDKKLLFLPSHREKGMEGTLEVR"
     gene            3761..4678
                     /gene="htpX"
                     /locus_tag="PK589_16405"
     CDS             3761..4678
                     /gene="htpX"
                     /locus_tag="PK589_16405"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNJ21245.1"
                     /translation="MKSLKGIGLLIISNILIYLTLSITVRVLVNMVLPAFGIDVRGAF
                     SQELLVWSLVIGFGGAFISLLFSKQMARAMLDCTQITEPRSHAEQVIYGSVREIAERL
                     QITMPEVWVYESPDPNAFATGPSKNNAMVAVSTGLLANLREDEVKSVLAHEMGHVFNG
                     DMFSTTVLAGLMNTFVHYISNFVYQMVAQPQGGDREEGQSGSPILGFVVYIFLQVVLS
                     VLAMLVVSWHSRRREYAADAFAAKVYGKDSMIKALQAIDRWVNRAQFEYSTQDALATM
                     KISGNTSGFMHLLATHPPIEERVAALERL"
     gene            4822..5319
                     /locus_tag="PK589_16410"
     CDS             4822..5319
                     /locus_tag="PK589_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNJ21246.1"
                     /translation="MAANASSVYDFTLNDIDGKPVSLSQYKGKVVMLVNTASFCGNTP
                     QYADLEQMYETYKDKGFEILAFPANNFGQQEPGSNEEIKGFCLTKYSVGFPLFSKISV
                     KGDDKHPLYRYLTEQSPFPGEVEWNFQKYLIDRSGNVVARYKHGTKPLAQEIVKDVER
                     FLAKG"
     gene            complement(5326..5601)
                     /locus_tag="PK589_16415"
     CDS             complement(5326..5601)
                     /locus_tag="PK589_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21247.1"
                     /translation="MKETDLEKLTLLYERFCDVCLVEKEVWTEIYMPRTFKDGTAVRT
                     NIQDRYQVMIDDQGVEDALEANIPLGKAALAAAIQEYREHISFEKQS"
     gene            complement(5688..6347)
                     /locus_tag="PK589_16420"
     CDS             complement(5688..6347)
                     /locus_tag="PK589_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21248.1"
                     /translation="MVAREGTRFSFPLALALCVTSILGWWQTGAEGRDLMVPKEQATT
                     EFEPTETPSLDVQQKGVPQSLFTWIKMARGYEVEWDTFGRKGWFTQDPRLKPIAPGTT
                     VFTPDTPAVYIVFEVAPLEDPAQFAAQVFAIGADGKTGDAVIVQDSLEVPGHERYGFL
                     ELKKPSSQWPQGKYVVKIYITSLGQQPFHAVNQVGTMRFAVTDQPSADVPPVVPGSPA
                     R"
     gene            6530..7954
                     /locus_tag="PK589_16425"
     CDS             6530..7954
                     /locus_tag="PK589_16425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ21249.1"
                     /translation="MNHPTTAAPPSLSQPVLLPNETETAEHEAKASSRQYGIRDAACQ
                     AAAQGGGENYFSAFALLLHASPFHIAILSALPQLVGVVAQLASVKVLRYLRLPRRLLI
                     AGGRAQAFCWLPILSLPLIDPQHGPWLLIGCAMLYFGFGHVTAPVWNSLLVDVAEADS
                     RGAYFAQRARTTALTSFLALGLAGAILTLGQRWDMSWIGFLVIFLGAAAARMGATRCQ
                     THVSRLMPVHQLDAPHGFRHFLANTASRDFRRFLLFSGLMHFAVLLSGPFFVIYLLRD
                     LHWSYLEYAGWMASSISAQFLTLGPWGRIGDRYGNKALLALTASAVPFLPMGYLLSEH
                     YLFLLTVNFFGGVIWAGLSLGLQNYVFDSLQPEERTRGVALANAMNAVGWGLGALTGS
                     WLATQMPTELSIGFWHLTPASNLPFLFCLSGVLRLAIAMSLLRTFAEPRRIASPPQGH
                     LVWELPVLKSLMTLMPWRNPRVAP"
     gene            complement(7979..8185)
                     /locus_tag="PK589_16430"
     CDS             complement(7979..8185)
                     /locus_tag="PK589_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21250.1"
                     /translation="MGQTNSSAAARVSGLVGIIVAAMVLVVGCASDKPKPMAQPSPEQ
                     VKGNADRAFDRLKQEEGERRPSGM"
     gene            8347..8796
                     /locus_tag="PK589_16435"
     CDS             8347..8796
                     /locus_tag="PK589_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNJ21251.1"
                     /translation="MSLHDRLSEDLKSAMKSRDQLRMDVIRMIKAAVQYKEVELKQDL
                     DDAGMSRIMTTLIKQRREAAEQFEKGNRQDLAAKERQEISIIEGYLPAAVSSDELANI
                     VAQVIKESGASSLKDMGQVMKAVMARLAGQSVDGKVVSDLVKTALQR"
     gene            8954..9601
                     /locus_tag="PK589_16440"
     CDS             8954..9601
                     /locus_tag="PK589_16440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ21252.1"
                     /translation="MSILIVDDSPDQQLLLKTILNKAGQHDLLIADSATSAYDQLGIQ
                     QDPSQEGPHAVDLILMDFLMPGIDGVAATRHIKSLDTLRDIPVIVVTAKTALGDLQAA
                     FAAGAMDYITKPVNSVELLARVNSALMLKKEMDCRKNRELELRRSNEDLQRALREVKV
                     LKGLVPICASCKKIRNDQGFWQQLEEYIQQHSEAEFSHGLCTPCIKKHYPGVYPD"
     gene            9646..10650
                     /locus_tag="PK589_16445"
     CDS             9646..10650
                     /locus_tag="PK589_16445"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNJ21253.1"
                     /translation="MGILIVDDSTDDRLLIQSILANAGYEETFVAESAAAAFRLLGLD
                     GMRQMAGRVDLILMDIVMPSTNGIEACRQIKAVERYRDIPIIMVTVKTDPVDLQLAFA
                     AGAIDYVAKPISKIELLTRVRCVLRLVHEIERRRAREQELLEVMRQLQEANQMLLRLS
                     CLDGLTGITNRRQFDDFLDQEWRRAARESTPLSLIMLDIDFFKTYNDSHGHQAGDECL
                     RQVAQLISSHVNRPGDLVARYGGDEFVIVLPGTTVDGAAQVADTLRRKVCEAEMKHPN
                     GDPVTISLGFASTVPSRVAAPTDLIRAADQALYQAKQEGRNRAKQAGVLSLVHHTHKD
                     "
     gene            10740..12509
                     /locus_tag="PK589_16450"
     CDS             10740..12509
                     /locus_tag="PK589_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ21254.1"
                     /translation="MAKRISQTRRTTGTTPKRTLRTSSRPQTALQREQAARRRAERAL
                     DRASKELQRRVEELQRAKDAADVANRAKSDFLASMSHEIRTPMNAIIGMADLLWDTTL
                     TPEQRKYLRIFRRAGASLLSLINDILDLSKVESGRLDLDSIDFDLSELIDKASEMLAL
                     RANEKGLELVCHLSNDVPCHLIGDPNRLYQILLNLLGNAIKFTEKGSVVMTITNDPDC
                     QTPGAIRFSVTDTGIGVPHDKMTAIFDTFTQAHSTIARRYGGTGLGLSISQQLAALMG
                     GKIWVESTLGKGSTFHCLVRLGVQAAPAPSKGAGALNLSGVRCLVVDDYPTNRLILRE
                     TLFAWGATVVEAESGEQAMEVLESALTSQTSFDLLLLDCRMPGMDGFEVIERIHADPR
                     LRNLTIIMLASDRWADDIAKTYDLGLGGYLVKPIRRSDLQQTISIALGRSQRGLAPSQ
                     STAPPQAAPQGRSLRILLVEDSPDNQVLIQSYLKNTTHRLDLADNGQIGVAKFQNGHY
                     DLILMDMQMPVMDGLTATRTIRRWEQEQGLPAVQIVALTALALKEESARIFEAGCNAH
                     MTKPLKRTTLLELLSAYEKTHAQ"
     gene            12506..12835
                     /locus_tag="PK589_16455"
     CDS             12506..12835
                     /locus_tag="PK589_16455"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNJ21255.1"
                     /translation="MMPPQPEAGQPKIIVHIDPDLEAIVPGFLANRRRDLVTIEACLK
                     QSDLNTIRILGHRMKGDGGGYGFDQISSIGDDLEQAVMVQNGPLIEKQVALLKDFLSR
                     VEVVYTR"
     gene            complement(12979..>13776)
                     /locus_tag="PK589_16460"
     CDS             complement(12979..>13776)
                     /locus_tag="PK589_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ21256.1"
                     /translation="QPPDVGPRTRDQASQERVVTRDRAGDRVVVDERVVERRREGEVY
                     VAGYGGFTWGHSFSDLEGRGTIAGQNFGGRDLANSGVYGAKIGYFHPGRLNWLGIEVE
                     GFNSTPHIEQGGGLPGSHLRVTTLAFNVIARTKVGCRNRDDVRHRDEVRHRDDGTVVE
                     SDYDRRQWSPLDENLRCPLQLYAGAGPGIFFAETSNQFGRSTDNGRIGLNALAGAKYF
                     MTRNLAVFAEYKFNYAQFDFSQMQGTGAGFNGEYKASHVVGGLALHF"
BASE COUNT         2963 a         4243 c         3792 g         2778 t
ORIGIN      
        1 aaggaagcga ggtgcaaata agtaaacagg acctgaccgg gccggaacaa cgcacattcg
       61 gctagctgcg gctccttcac tttcacgatg agttcggccc gtttgaacac ctcttccttg
      121 gagcccacga cgtccgcgcc ggcttgacga tacgcctcat ccgaaaaccc gctcccttgt
      181 ccggccgatg gctcgaccaa cacctgatgg ccatgctgtc gcaacagacg cacgccgtca
      241 ggcgtcacac tcacgcggta ttcgaaatct ttaacttctt taggaacgcc aatcagcatg
      301 cgcgcctcct cgccccagtc accagtgctc ctgttgttgt ctacattata ccccgaaccg
      361 cactctgtat atgcgtgcct cgctccacca ccttgcgagg ggaaagagca gtgcggtaag
      421 attcgatctc tttggttctt tccccttgaa gaggagcact tcgcgcatgg atgccggatc
      481 ctgtaatgcc tgcaacaccg caggtacacc gttgatgaaa ttgtcgttgg ggaaagattt
      541 tttcggtcgc acctatgacc gattgtcgcc ctcctctgac cagagcccga aatggtattg
      601 tgaaccctgc tccatgatga aaaatctgca gcgcgacttt cgtgatatcc ggacggaatt
      661 cgacaaactc gccacgggac aggcctctgc cctgtctgag ccagaggcga aacagcgcgc
      721 gcaattgcgt cttcgagaaa tttccgccat tgccggcagt caaactgccg gctcgtcact
      781 cctgaaccca gcggacgtga agcaactgat cgaccagttc cacgcgcgtg cctgagctcg
      841 caattaccag cttgaaccac ataacacact gacatcctga cgatgaccgg ctatcaacga
      901 catatatttg tatgcaccaa caagcgcgag cctgatgatc cccgcggcag ctgttccaag
      961 cttggatcag agtcgcttca tgcctgtttc aaacaagagg ccaagcgact caatttgaag
     1021 ggcatcgtgc gtgcgaataa ggccggttgc cttgactatt gcgcccaagg acccagcgtg
     1081 gtggtctatc ccgaaggcat ctggtaccgc gtgcggactg aagccgacgt gaaggaaatc
     1141 atggagcgcc atatcgtgaa cggagaaccg gtcgaacgac tcctgatgcc tgatcaggcc
     1201 cggccttccc tcctctctcc gctgaaacct tgagccgggt cgtccaccat agctcgcgag
     1261 atctgtatgc cagccgatga aaaatggaag gccaacatgg aaaaggtggc cttcatgaaa
     1321 gcgtttcccg gtttgctcag cacctgggat cagctgaagg gcaaaaccgt ggaggccgtg
     1381 ttaccgctca agagcaagac cggatcctcc gcgatcatct gcaccgatgg atcgttcgtc
     1441 gtggtcccgc tgctgagtcc ggaaccatac gagttaggcg aagcgcttca ggcaggccga
     1501 gagtacctcg agccgaagca ccctgaggcc tacgtcggat atgaccggtt gcgcaagaag
     1561 gaccaagatg cgcaacgcac cgcgcgcctc gaaaatattc tcggcgccat tcaaaataat
     1621 gtggagcaga ttccggaatt gaaggaccgg ctggcacagc tggtcaagga gtggaagtga
     1681 gcagtctccc gcaaaaaaac atgttcgaga tcttttcgca gggtctcttc gaaggagtga
     1741 aaccgatgct ggttatccgc gatcatcttg tccgccatcc tgaccgctgc acccaccaag
     1801 ccatttgcgt gcccatttgc ccgaccagcg cctggctttc gacccctccc tacaaattcg
     1861 acccgtcccg ctgcctggaa agctgccggc tctgcctgga tgcctgcccc tcccaagcga
     1921 tctatgccgt gttcagaaaa ggtgagaaac tgttagagcc gcagaagaag taacgggtcg
     1981 atcgacgccc tacgcgtcta catgatctgc tttcgtaaac gcccccagta ggccgtcccc
     2041 acatagccgc aaccgccggt cacggtcgtg agcgccatca ctcggtagac caccgagcgc
     2101 ggggctttga cattcacgct ggggtcgagc agatcgggcg cagtggccac acgtttcagc
     2161 gtttcgccgg ccaacagaat cggccacatg caggcaaggc gttgccggat ttccaaccgc
     2221 ggcagcgcca tggtgtagag ccaaccctga tccaaatgtt ccaccgcctg acggatcatc
     2281 cgcaggagga tggggcgaaa ggtcggaagg ctttcgggtt tgagcagatc cgtcggtttg
     2341 agccccactt catccagcca ctgcgtcggc acgtaacacc gaccgttgtg aaggtcgcgg
     2401 gcgacgtcct tcacaatgtt cgtaagctgc aatcccttac cgaagcggac gccgatcgcg
     2461 gacatccggt cgacatccca gcgcctcatt gccggccgat gtgcgcagac catgcgggtc
     2521 caaaactctc ccacacaacc ggcgacgaaa tacgtatacc gatccaattc ttcgggacgc
     2581 tccagcgcgg tgaggtcctg ggctgatgaa ccgggaaacc tggccaggtc catttccatg
     2641 ccgttcggca gcacctccat gagccagcga atgcgatcgc gatccccctg ctcaaactgc
     2701 ctgtaaagag ccaagcactc cggcagccgc tccagaagca cgcgctcagc cgagtcggtc
     2761 tggtgcggca gcagtccggt ctgaatggcc tgcacggcct gcggctgaac ctcgtcgcct
     2821 gcgaactgcg cgcgaaacat ggtgagatat cgaaggcgct ccgcccgccc gatcagctcg
     2881 gtgtcggcga tggtgtcggc cgctcgcgcg agcagatagg ccaggcccat ctcgtcgcgc
     2941 acatccgacg ggaggacatt caacgtcagg taaaatgaac gggatacctg cttgagaatg
     3001 acgtggagga gttcgtgctt ggaaggctga atactcgtgg cgcaccctgc ttatctgact
     3061 tcgagtgtcc cttccatacc tttctcccga tgacttggaa gaaataggag ctttttatcg
     3121 caatagaagg caaacctacc cggcttcgtc ggagtaaatt gcacggtgac cgttttaccg
     3181 gcgctgacat cccgttcgat cgagagcccg gcggcctcat cccgcaagac aaaattgtgc
     3241 ggagtgatcg tggtgatact ggtcaacagc agttcgacgg gtttcccggc ctgcacgatc
     3301 aggtgctgcg gggtatagga gtaactgtcc aggatcaccg tggctcgctg cataccgtcg
     3361 gcttgatcga ccggcacgcg caatggaggc acctgcagcg cctgctccgc ccccaggaca
     3421 gcagccggac ctgcgacgac gccgagtcct cctgtcccca acatacccaa gaccagtggc
     3481 aaaagaccta tacacatagc cgagtttcta gcagaagggc gcagaagtgg tcaaccggcg
     3541 acagcgctac ccatgcttca ggcagcgatc gaagccagag catccagacg cctcgccctc
     3601 cacagacttg acggacccaa aacctggtat tatctgtctg catcgggacg tgtgccggct
     3661 cctctgcccg gggggccaac aaccggaccg acattcggta taatgacatc caaggtttgg
     3721 ttctctccgt agaagaagga caaacctcca aggaggagat atgaagtcgt tgaagggtat
     3781 cgggctgctg attatttcga acattcttat ttacctcacg ctgtcgatca ccgtcagagt
     3841 gctggtgaac atggtgctgc ctgcattcgg catcgatgtt cgcggagcct tcagtcagga
     3901 gctgctggtg tggtcgttgg tgatcggctt cggcggcgcc tttatcagct tgctgttctc
     3961 caaacagatg gctcgcgcca tgttggattg cacccagatc acagaacccc gctcacatgc
     4021 cgaacaggtg atctacgggt cggttcgaga aattgcggaa cgcctccaga tcaccatgcc
     4081 ggaagtctgg gtctatgagt caccggaccc caacgccttt gcgaccggac cgagcaagaa
     4141 caatgcgatg gtggcagtct caaccggctt gttggcgaat ctgcgtgagg atgaggtgaa
     4201 gtccgtcctc gcccacgaga tgggtcacgt cttcaacggc gacatgttct ccacgaccgt
     4261 gctggcgggg ttgatgaaca cctttgtgca ctacatcagc aacttcgtct accagatggt
     4321 ggcccagcca caaggcggtg atcgcgagga aggccagagc ggaagcccga ttctgggatt
     4381 cgttgtgtat atctttttgc aggtcgtgtt gtcggtcctc gcgatgttgg tggtcagttg
     4441 gcactcgcgc cgccgggaat acgcggccga tgcctttgcg gcgaaggtgt atgggaaaga
     4501 ctcgatgatc aaggcgctcc aggcgatcga ccggtgggtc aatcgcgcgc agttcgagta
     4561 ctcgacgcag gacgccttgg ccacgatgaa gatctccggc aacacatccg gatttatgca
     4621 cttgctggcg acccatcctc cgatcgagga gcgtgtggcg gcgctggaac gtctctaact
     4681 gaaggagtcc atacaatgcc gatctataga ccgacttgtt catggaaggt tctggttctt
     4741 gggctcgcag tgggtctcgg gctaggcgcc gtggaactgg gcctgggttc ggtcgatgcg
     4801 gcgcaacaag gagcaacacg catggcagca aacgcttcaa gcgtctacga tttcacgtta
     4861 aacgatatcg atgggaaacc ggtttcattg agccaataca agggcaaggt cgtcatgctg
     4921 gtcaatacgg ccagtttctg cggcaatacc ccgcagtatg ccgacctgga acagatgtac
     4981 gagacttaca aagacaaggg ctttgagatc ctggccttcc ccgccaataa tttcggccag
     5041 caggagccgg ggagcaacga agagatcaag gggttctgcc tcaccaaata tagtgtcggc
     5101 tttcccctct tcagcaagat cagcgtgaag ggtgacgaca aacatccgct ctaccgctac
     5161 ctcacggagc agagcccgtt ccccggcgaa gtggaatgga actttcagaa gtacctgatt
     5221 gatcgttcag gcaacgtcgt tgcccgctac aagcacggca cgaaaccgct ggcacaggaa
     5281 atcgtcaagg acgtcgaacg gtttctcgcg aaaggctaag cgccgtcagg actgtttctc
     5341 gaaactgatg tgctcgcgat actcttggat ggccgcagcc aatgcggcct tcccaagagg
     5401 aatgttggcc tccagcgcat cctccacgcc ctgatcgtcg atcatcacct gatagcgatc
     5461 ctgaatattg gtccgcaccg ccgtgccgtc cttgaacgta cgcggcatgt atatttccgt
     5521 ccacacttcc ttctcgacta aacacacatc acaaaaccgt tcatacaaca gcgtcaattt
     5581 ctccagatcg gtttccttca tcccctgctc ctcgcgataa acaatacatt caaagaccgc
     5641 acgagcccac gcggcagacg aacgttagac taagcagggg aagatattca acgggcggga
     5701 gaacccggca cgacgggggg cacatctgcg gaaggctggt cggtcacggc aaatcgcatc
     5761 gttcccactt gattcacggc atggaacggt tgctgcccga gcgacgtgat gtagatcttg
     5821 accacatact tgccctgtgg ccactgcgac gaaggcttct tcaactccaa aaaaccgtat
     5881 cgttcgtggc ccggtacctc gagactatcc tgcacgatca ccgcatcgcc ggttttcccg
     5941 tcagccccga tggcaaacac ctgggcggcg aactgggccg gatcttcgag cggggcgact
     6001 tcgaacacga tgtacacagc cggcgtatcc ggcgtgaaga ccgtcgtacc cggcgcgatg
     6061 ggtttcagcc gtggatcctg cgtgaaccac cctttgcggc caaacgtatc ccactcgact
     6121 tcatagccgc gcgccatctt gatccaggtg aagagtgact gcgggacgcc tttctgttgc
     6181 acatccagtg acggcgtttc ggtgggttcg aactccgtgg tggcctgctc tttgggaacc
     6241 atcagatcgc gaccttcggc gcccgtctgc caccatccca ggatcgaggt gacacagaga
     6301 gcgagcgcca atgggaagga gaaccgcgtc ccctcacggg caaccaagtc tgctgctcct
     6361 tgtgaaaccc gggcatccat acagttatgg tacccactgt gagaacaggg ggtcaaccgg
     6421 cctgcgcgat ttgatcaaaa cagaaggcaa cgcggttcgc tctgatataa tgcctttcat
     6481 tcaacgcaaa attcgtaggc atcgcgcact tgttcccgcg caaagtccca tgaatcaccc
     6541 aacgaccgcc gccccgccct cactttctca gccagtccta ttgccgaacg agacggaaac
     6601 cgcggagcac gaggccaagg cgagcagccg gcaatacggc attcgggatg cggcctgcca
     6661 agccgcggcc caaggcggcg gggaaaacta tttctcggcg ttcgccctgt tgttgcacgc
     6721 ttctccgttt catatcgcca ttctgtccgc gcttccgcaa ttggtcggag tagtggcaca
     6781 actcgcctcc gtgaaggtgc ttcgctatct tcgtctgcca cgacgcctgc tcatcgccgg
     6841 aggccgggcc caggccttct gctggcttcc gatcctgtcg ctgccgctga ttgacccgca
     6901 acatggccca tggctgttga tcggctgtgc catgctctat ttcggctttg gccatgtcac
     6961 cgccccggtc tggaatagtc tcttggttga cgtcgcggaa gccgacagtc gcggcgccta
     7021 tttcgcccag cgtgctcgca cgaccgcgct cacgagtttt ctggcattag gactggccgg
     7081 ggccatcctg acgctggggc aacgatggga catgagctgg atcggcttcc ttgtcatttt
     7141 cctcggcgcc gctgccgcgc gtatgggagc cacacgttgc cagactcacg tgtcacggct
     7201 gatgccggtc caccagctcg atgcgccgca cggcttccgg cattttcttg ccaacaccgc
     7261 cagccgggat tttcgtcgct tcctcttgtt ctcagggttg atgcatttcg cggtactgct
     7321 gtcgggaccg ttttttgtga tctatctcct gcgagacctt cattggtcct atctcgaata
     7381 tgccggctgg atggcgagca gtatctcagc tcaatttctc acgctgggcc cctggggtcg
     7441 aatcggtgac cgatacggca acaaagcctt actggcgctg acggcctcgg cagtgccgtt
     7501 cctgccgatg ggctacctgt tgagcgagca ctacctgttt ctgttgaccg tcaacttttt
     7561 cggtggcgtg atctgggcgg gactgtcgct cggcctgcag aattatgtct tcgattcatt
     7621 acaaccggaa gagcgcacca gaggggtcgc gctggccaat gccatgaatg cggtgggatg
     7681 gggactcggc gcactgaccg gcagttggct ggcgactcag atgcccactg aactctcgat
     7741 cggcttctgg catctgacgc cggcgtcgaa cctgccgttt ttgttttgcc tctcgggagt
     7801 cttgcgactt gctattgcca tgagtctgct gcgcacgttt gctgagcccc ggcgcattgc
     7861 ctctccgcca caaggacatc tggtctggga actgcccgtc ctgaaatccc tcatgacgct
     7921 catgccctgg agaaacccac gcgtggcacc ctagagggcg cacagcccga agccaggctt
     7981 acatccccga tggccggcgc tctccctctt cctgtttcaa ccggtcaaac gcacgatccg
     8041 cattcccttt gacctgctca ggtgacggct gagccatcgg tttcggtttg tcgctggcac
     8101 agcccaccac aagcaccatc gccgcaacaa tgataccgac gagcccgctt acccgcgccg
     8161 ctgcactgct gtttgtctgc cccattctga gcctccgtgg ttaagtgcca acgctaccgc
     8221 cgattgcatt atgggacagg acctcccggc gctcaataca aaacgtgcgc gcctcagcca
     8281 cgttgactgg ctccggtaaa ggcgttattc tccgcgacga cgcatttccc tgctgaaagg
     8341 aacccgatgt cgctgcatga tcgcttatcc gaagacctga aatcggccat gaagtccaga
     8401 gaccagctgc gtatggacgt cattcgcatg atcaaagccg ccgtgcaata taaggaagtc
     8461 gaactcaaac aggatctcga cgatgccggc atgagccgga tcatgaccac tctcatcaaa
     8521 cagcgcaggg aagcggccga gcaattcgag aagggcaatc gacaggactt ggccgcgaaa
     8581 gaacggcaag agatcagcat catcgagggc taccttcctg ccgcagtctc ttccgacgag
     8641 ctggcgaata ttgtcgcaca ggtcatcaaa gaatccggtg catcgtcgct gaaggacatg
     8701 gggcaggtca tgaaagccgt catggctcgt ctggccgggc aatccgtaga cggcaaagtc
     8761 gtcagtgacc tggtcaaaac cgcccttcaa cgctgatccg cctcgctgcc ggacttaacc
     8821 tcctcggtct tttcgccgat actgaatccg acgtacggcg gcgaaggggt atgccctcac
     8881 gcctctcctc cattcgtgat gctcccggga accaggagcc ggctccgcca cctctgcccg
     8941 acgaggcgat gacatgagca ttttgattgt cgatgactcc cctgaccagc aactgctgct
     9001 caagaccatt ctcaataaag caggccagca cgacctgctc atcgcggact ctgcgacctc
     9061 agcctacgac cagctcggca ttcagcagga tccgtcgcag gaagggcccc atgccgttga
     9121 tttgattctc atggattttc tcatgcccgg catcgacggc gtggcggcga cgcggcacat
     9181 caagagcttg gacacattgc gggatattcc ggtgatcgtc gtcacagcca agaccgcgct
     9241 gggcgacttg caggcagcct ttgccgccgg ggcgatggat tacattacga aaccggtgaa
     9301 cagcgtggaa ttgcttgccc gggtcaattc cgcactcatg ctcaaaaagg aaatggattg
     9361 ccgcaaaaat cgcgagctgg aacttcgccg gagcaacgaa gacctgcaac gggcgttgcg
     9421 cgaagtgaag gtactgaagg gcctcgtgcc gatctgcgcc tcctgcaaga aaatccgcaa
     9481 tgaccaaggc ttctggcaac aattggaaga atatatccaa cagcattcag aggcggaatt
     9541 cagccacggc ctctgcaccc cctgcatcaa aaagcattac cctggtgttt acccagacta
     9601 gctgctagca tcgacctacg acatattttc agggagacac tgatcatggg tatcctgatc
     9661 gtcgatgatt cgacagacga tcgtctgttg atccaatcca tccttgccaa tgccggatac
     9721 gaagagacgt ttgtggcaga gtccgccgcc gccgcgtttc gtttgctggg cctcgacggg
     9781 atgcgccaga tggccggccg tgtggatttg atcctgatgg atatcgtcat gccgtccacc
     9841 aatggcatcg aagcctgccg ccagatcaag gccgtcgagc gataccggga catcccgatc
     9901 atcatggtca ccgtcaagac cgaccccgtc gatctgcaac tcgcctttgc tgcaggcgcc
     9961 attgattatg tggcgaagcc gatcagcaag attgaactct tgacccgcgt ccgttgcgtg
    10021 ctccgcctcg tccatgaaat cgaacggcgc cgggcacgcg agcaggaatt gctcgaggtc
    10081 atgcgccagt tgcaggaagc caaccagatg ctgctgcgcc tctcctgcct tgacgggctt
    10141 accggcatca ccaatcgccg gcagttcgat gacttcctcg accaggaatg gcggcgcgcc
    10201 gcgcgcgaat ccaccccgct gtcactcatc atgctggata tcgatttttt caaaacctac
    10261 aacgacagcc atggccatca ggccggggac gaatgcctcc gtcaggtcgc ccagttgatc
    10321 tcaagccacg tcaatcgtcc cggcgacttg gtcgcacggt acggcggcga cgaattcgtc
    10381 atcgtgctgc ccggcacaac tgtcgacgga gccgcacaag tcgccgatac cctcagacgg
    10441 aaagtctgcg aagcagagat gaaacatccg aacggcgatc cggtcacgat cagcctgggc
    10501 ttcgccagca ccgtgcccag ccgcgtcgcc gccccgaccg atctgatccg cgcggccgac
    10561 caggcgctct atcaagccaa gcaggaaggg cgcaaccgcg cgaaacaagc gggtgtgctg
    10621 tccctcgtcc accatacgca taaggactga cagttactcg ccgtcgcgac gatcgtgccg
    10681 gcttcctcta ctcgcgatac gatatggtta tgaccatctg aaggcgaccg gacgacgaca
    10741 tggcgaaacg catctcgcaa accagacgca ccacaggcac cactcctaaa cgcacccttc
    10801 gcaccagttc ccgtccacag acagcactcc aacgtgaaca agccgcgcgc cggcgtgccg
    10861 aacgcgcgct tgaccgcgcc tctaaggaat tgcaacgccg cgttgaggag ctgcaacgcg
    10921 ccaaagatgc ggccgacgtg gccaaccgcg caaagagtga tttcctggcc agcatgagtc
    10981 atgaaatcag gacgccgatg aacgccatca tcggcatggc tgatctgtta tgggatacga
    11041 ctttgacccc ggagcaacgg aagtatctcc ggatctttcg acgcgcgggc gcaagcctgc
    11101 tgagtctgat caacgacatt ctcgatttgt caaaagttga atccggccgg ctcgatctcg
    11161 atagcataga cttcgacctc tcggaactca tcgacaaagc gagcgagatg ctggcgctcc
    11221 gcgccaacga aaaaggcctc gaactggtct gtcacctgtc gaacgatgtg ccgtgccacc
    11281 tcatcggtga tcccaaccgc ctctaccaga ttctgctcaa cctgctggga aacgcgatca
    11341 agttcacgga gaagggatcg gtggtcatga ccatcaccaa cgatccggac tgccagacac
    11401 ccggcgccat ccgattctcg gtgactgata cgggcatcgg ggttccccac gacaaaatga
    11461 cagccatctt cgacaccttt acgcaggcgc acagcacgat cgcccgccgc tatgggggca
    11521 ccgggctggg attatccatt tcacagcaac tggccgcact catgggcgga aagatttggg
    11581 tcgagagcac cctcggcaag ggcagcacgt tccattgcct cgttcggttg ggcgtacaag
    11641 ctgcgccggc accttcgaaa ggcgcggggg ccctgaacct gagcggtgtg cgctgtctcg
    11701 tcgtggatga ttacccgaca aaccgtttga ttctgcggga aacgctcttt gcctggggcg
    11761 ccaccgtggt ggaagccgag agcggagagc aggccatgga ggtgcttgaa agcgcgctca
    11821 cctcgcagac gtccttcgat ctccttttgt tggactgccg catgccggga atggatggat
    11881 tcgaggtcat cgaacgtatc catgccgatc cccgccttcg caacttgacg atcatcatgc
    11941 tggcctccga ccgatgggcc gatgacatcg cgaagaccta cgacttggga ctgggcggat
    12001 acctggtcaa gccgatccgg cgatccgatc tccagcaaac tatcagcatc gcgctggggc
    12061 gcagccagcg gggattggct ccctcccaat ccacggcacc accccaggcc gcacctcagg
    12121 gacggtcttt gcgcatcctg ctggtcgagg actcaccgga caatcaggta ttgattcagt
    12181 cctatctcaa gaacacgact catcggttgg acctggccga caatgggcag atcggcgtcg
    12241 ccaaatttca aaacggtcac tatgacctga ttttgatgga tatgcagatg ccggtgatgg
    12301 acggactgac tgccacacgc acgatccgtc gctgggaaca ggaacagggg cttccggccg
    12361 tccaaatcgt cgccctgacc gccctggcgc tgaaggaaga atcggcgaga atttttgaag
    12421 ccggatgtaa cgcccacatg accaagccgc tcaaacgaac gacgctgctg gaactcctat
    12481 ccgcctacga gaagacccac gcgcaatgat gccaccgcaa cctgaggcag gacagcctaa
    12541 gatcatcgtc catatcgatc cggatctcga agccatcgtc ccaggcttcc tcgccaaccg
    12601 ccggcgtgat ctcgtgacga tcgaggcctg cctgaagcag agcgatttga atacgattcg
    12661 catattgggc catcggatga aaggcgacgg cggcgggtac gggttcgatc agatcagcag
    12721 catcggggac gatctggagc aagccgtgat ggtacaaaac ggaccgttga tcgaaaaaca
    12781 agtcgcgtta ctgaaagatt tcttgagtcg cgtggaagtc gtctatacac gctagatcct
    12841 cgcacctggc acctccttcc cctctacacg aagccccgtc cctgacgacc gtgagaagtt
    12901 acgcgcagag ccaggcggcg cgggctcaac ggtcatgtcg gtgaccgctg aaccctggag
    12961 cgtcacgcgg caatcatgtc agaagtgaag tgccaatcct ccaaccacat ggctcgcttt
    13021 gtattcgccg ttgaaccccg ctcccgtgcc ctgcatctgc gagaagtcga actgcgcata
    13081 gttgaatttg tactctgcaa agaccgcaag atttcgagtc atgaaatact tcgctcctgc
    13141 cagcgcattc aagccgatcc gcccgttgtc agtactgcgg ccgaattgat tcgacgtttc
    13201 ggcaaagaaa attccgggac cagcaccggc atacagttgc aaggggcagc ggagattttc
    13261 gtccaatggc gaccactgtc tccgatcgta gtcggattcc accaccgtgc cgtcatcgcg
    13321 atgcctgact tcatcccgat ggcgcacgtc atcgcgattt ctgcaaccga ccttcgttcg
    13381 ggcaatcaca ttaaacgcca gcgttgtcac ccgaaggtgt gatcccggca accctccgcc
    13441 ctgctcgatg tgaggcgtcg aattgaaccc ttccacctcg atgccgagcc agttcaaccg
    13501 acctggatga aaatagccga tcttcgcgcc atacactccg gaattcgcca gatcacggcc
    13561 tccgaagttc tgacccgcga tagtgccccg gccttcgaga tccgagaagc tatgtcccca
    13621 cgtgaaacct ccataccctg ccacataaac ttcgccttcc ctgcggcgct ccaccacccg
    13681 ctcatcgacg accacccggt cacccgcgcg atcccttgtg acgactcgtt cttgagatgc
    13741 ctgatcgcgc gtccgtggac cgacgtccgg tggttg
//
LOCUS       DAOQDS010000089        12824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_2269_length_12824_cov_18.067429, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000089 DAOQDS010000000
VERSION     DAOQDS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12824
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2269_length_12824_cov_18.067429"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..561)
                     /locus_tag="PK589_16465"
     CDS             complement(112..561)
                     /locus_tag="PK589_16465"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ21257.1"
                     /translation="MMMDGRQWAPILATAAILMVAVVSPALADPASEHQLQALAGQGD
                     ANAQWMLGQALLTGNLGTTDEAEAVRWLQLAADQGHVLAQRDMGMLYEQGQGVTQDTL
                     EAFFWYSLASRQDSGRAKLRRDVLAAMLTPDQREAVAARLKGWRPSR"
     gene            complement(576..1826)
                     /locus_tag="PK589_16470"
     CDS             complement(576..1826)
                     /locus_tag="PK589_16470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNJ21258.1"
                     /translation="MSSSARVIIIGAGLAGLACAQHLVRRGLACTVLEASDEAGGRVR
                     TDRVDGFQFDRGFQVLLTGYPEAAKVLDYPSLQLQSFHPGALVRYGGRFHRISDPLRR
                     PQDLVAGLMSPIGSFRDKLTILRLRQDALNQRLCAQAGGGNRTTLEALDRYGFSAVMR
                     ERFLRPFLGGVFLDQSLSTPCRHFELVWAAFSRGAIALPQAGMGAIALQLASSLPAGS
                     IRLRAPVTQLDGKQVVLASGERLSADAVVLATDYATAATLRGEPVPAAPGRRSTTLYF
                     DAPTPPVPGPWLLLNGEQQGLIGTACVLSEVAPAYAPSGRALVSVSLADQTAPVDADQ
                     QQIVRAQLREWFGGAVDSWRHLRTDRISRALPPVDVLASRNGAASPRLRPALYQCGDY
                     CETGTLDGALLSGRHAAAAILADL"
     gene            complement(1836..>2171)
                     /locus_tag="PK589_16475"
     CDS             complement(1836..>2171)
                     /locus_tag="PK589_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21259.1"
                     /translation="SAEWMKVGGTHKYDGYVDMATIGPTGKTVTLWTLKDFKVDRQIP
                     QGTYRSVRIKKQIDCEGHKSRPLHNKYYASQMGKGRPMQSGKATREWSRVTPGSDGEA
                     EFKIACKKV"
     assembly_gap    2172..2271
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2272..3048
                     /locus_tag="PK589_16480"
     CDS             <2272..3048
                     /locus_tag="PK589_16480"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNJ21260.1"
                     /translation="RRGEPAAIRPTPPADLDREKRVTLDRLLSKLGIASRSQAQEWIR
                     AGRVRINQRLVRQPDTWVVWPGDAVTLDEQPLQQGAPRFILFHKPKGVVTTHADEQGR
                     RTIFDVLPPEFTRLHAVGRLDQATSGLLLLTNDTALSSFLTDPKQRIRRRYLVTVRGE
                     VTDDRAQAAIDGLEDQGERLHCAALTIQKRSGRESHLDVTLTEGKNREIRRLFNALGH
                     EVTRLRRIQYGPFALTDLEPGAWREIPIDAAKALLDKGLP"
     gene            3286..>3716
                     /locus_tag="PK589_16485"
     CDS             3286..>3716
                     /locus_tag="PK589_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21261.1"
                     /translation="MKEIMKRFATGLTTLAFAAALLAPALTYANGADKAPLEIKVRVS
                     EKGFFDEKGKPYGGKRMLQVPKDTMVKITFVFGEDLTSLAAGDTHQITVRAEDGWKQE
                     TSPLWMMNRESTVSFLAGEKGRMQYRAYCILDCIGMEHLTNL"
     assembly_gap    3717..3816
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3817..4070)
                     /locus_tag="PK589_16490"
     CDS             complement(<3817..4070)
                     /locus_tag="PK589_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21262.1"
                     /translation="MTKYVSRSIIGISLGLLLWSGTGLATEPADVEKFVNARIEIGEM
                     MTNYFKGGESYGSGQRPSPEKMKEMGDDINGKLSDLLSKRG"
     gene            complement(4174..5088)
                     /locus_tag="PK589_16495"
     CDS             complement(4174..5088)
                     /locus_tag="PK589_16495"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ21263.1"
                     /translation="MLVSRILLVLALAGFVGCAGTTPPVRTNSGAHPPQLADVIPPDQ
                     PFQRHMVDLHGLHLSVLEAGTGDPIIFVHGVVTTSNIFPKYVGAYSPEFRGIAVDLRG
                     YGDSEKPATGYSIPQFSNDLIKLADVLQIKKAVWVGVSMGGMILQRLALDHPERVRAL
                     VLVSTTDGPMILDKDIPTIGAPRDYREVSKNMIVESFPADTQAKTYQPLLDRIPTWNG
                     TVIREALTSMSQFNVHGQLTRIQVPTLIMVGVKDDVATPAIAKGIQAQIAGSQVVEYQ
                     TGHFMMAEDPDRFRAVLGEFLKGLPPSQ"
     gene            complement(5203..5511)
                     /locus_tag="PK589_16500"
     CDS             complement(5203..5511)
                     /locus_tag="PK589_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21264.1"
                     /translation="MKDVTISPSLVARSFKSDRAKPLSRGLSDLAGWLFTVNADRWRR
                     RAQFYERHRDYFPRVAASPFVDRCRELEAASREFAEVLFAMPPRVDLVPIQVTVSRIK
                     "
     gene            complement(5645..6397)
                     /locus_tag="PK589_16505"
     CDS             complement(5645..6397)
                     /locus_tag="PK589_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ21265.1"
                     /translation="MAKITEVAPDLFRITTFLEPFNLQFSQFLVRDEQPLLFHTGPRA
                     LFADVKEAVASLIDVRTLRWISFSHFEADECGSLPDWQGVAPQSDAVCSLVGKLVSVD
                     DCLALRPAKGMADGEVLQTGKYRFQFLATPHVPHCWESGLLFEETQRTLLCSDLFHQA
                     GDGEPVTQSDVVGRCREVLMEYQQGPLANYMPYCTLTDPTLNRLAALKPNTLATMHGS
                     VYAGDGAQALRDLAHVFREILGSPAPVSMPSP"
     gene            complement(6497..6982)
                     /locus_tag="PK589_16510"
     CDS             complement(6497..6982)
                     /locus_tag="PK589_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21266.1"
                     /translation="MAWLCYAAPLLFMIGPAAVMAHEPLTSILFHSAIGFSLSALIHR
                     IGHALLTPLPKGPHAIYLSATRRLPLRLTLSQFVWLLEGLVALGLFCGLLSKRCWLAG
                     GTEMLVAGWVLFLLGGGLFFLPVRLVKLWEMRHYPATSLVGPSDDVINRSLSCLPRLF
                     K"
     gene            7393..7572
                     /locus_tag="PK589_16515"
     CDS             7393..7572
                     /locus_tag="PK589_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21267.1"
                     /translation="MKQQIQRPVRRTEQQKPTQDKDGVWPLSAREFMKLPVSGLLSTG
                     KRIRFTRLTPPDQAA"
     gene            7601..7789
                     /locus_tag="PK589_16520"
     CDS             7601..7789
                     /locus_tag="PK589_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21268.1"
                     /translation="MLIEDSLLTHDERKAAEAAFRGLPLDAQWSRGAQTIYIGILEVT
                     RGRDIVGDDLWEEVAAAV"
     gene            complement(7863..8207)
                     /locus_tag="PK589_16525"
     CDS             complement(7863..8207)
                     /locus_tag="PK589_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNJ21269.1"
                     /translation="MLNAEVMAEVLEFRRKRDWEQFHKPKELAAAITVEASELLEVFQ
                     WKTHEEVARLLGGPSRERVTDEIADVVILLSYLCHDLGLDVNEAVRAKLKKNEAKYPV
                     EKSYGNARKYDE"
     gene            8417..8965
                     /locus_tag="PK589_16530"
     CDS             8417..8965
                     /locus_tag="PK589_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21270.1"
                     /translation="MNRRLLSALSLLLMLTAPVYAQSPCSDCFYAAEEEVRKCLDNAI
                     SAGDRNSCLDNRRVRLKACSDNQCQTVREETAITETQSTVSRPGVAPYTPTEAEWLAL
                     ITRAGLRREATPDRPYSLDIILADPQTLQIMVRHASTMDREQLRKAIEGARETIRNTA
                     KSYGWDKWVKIRESVEMYAAKK"
     gene            complement(8993..9499)
                     /locus_tag="PK589_16535"
     CDS             complement(8993..9499)
                     /locus_tag="PK589_16535"
                     /inference="COORDINATES: protein motif:HMM:NF017517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HNJ21271.1"
                     /translation="MLSNPSREQSHARTLTSYPLNLQGRIYGSAMPFGLYDPEGTLLP
                     KFRAAAIHGVVLLAEPDECREKTGRDLLAMYREEGLTVLPLPIPNYGIPVDGGLPEVL
                     KRVHEEAVQGHNVVIHCSAGLGRTALFATLLARSVLGLSGPDALAWLGRHQPGALLTP
                     AQIMLIME"
     gene            complement(9489..10574)
                     /locus_tag="PK589_16540"
     CDS             complement(9489..10574)
                     /locus_tag="PK589_16540"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HNJ21272.1"
                     /translation="MNILQATHEYERWSARQVPFVPADLRLKHRMMTQSAFSFLRATC
                     YRWLQVWPEVCADLTAAPALRAVADLHVENFGTWRDSEGRLIWGINDFDEACVFPYAS
                     DLVRLVVSAKLAGRAEHLAVKLDEACDAVLTGYLEGLRAGGRPFVLSEQHAWLRDIAT
                     NSLRDPVKFWKKMAALPMLKDTVPPEVANALEKVMPEAGLSYGLRRRVSGLGSLGRPR
                     VVALADWRGGKIAREAKRLIASAGLWARGQKPIGMLLYQLMLEQSVRCRDPFVGMDGS
                     WLLRRLSPYCSRIEVTALPKKRDEGKLLYAMGWETANVHLGDRAQIKAVQRDLAARKS
                     AWLKKAAKAMLKTTVADWKDWAEHHAE"
     gene            complement(10702..11397)
                     /locus_tag="PK589_16545"
     CDS             complement(10702..11397)
                     /locus_tag="PK589_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNJ21273.1"
                     /translation="MMKTGRASFHVDHAAESRLGFPTVTVLPPKHFSMLREFHLADVL
                     TLGNAACGLAGVFFAMQYMSSHSLAHFLAAAALSPAALLFDWFDGRVARWRHQQSVLG
                     RELDSLADIISFGVAPAALAFAAGLRGGWDWIALTYFVCCGVSRLARYNVTAERLSAG
                     HDKVAYFEGTPIPTTALLTGVLAWAAWQDRLGEQLYGGVWTIGPGDLHALSLLFILSG
                     TLMISKTLHIPKL"
     gene            complement(11512..11829)
                     /locus_tag="PK589_16550"
     CDS             complement(11512..11829)
                     /locus_tag="PK589_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21274.1"
                     /translation="MMKRTLQMCLVAALLTGGGGFAFAADPMKDKEASPTLGERITKD
                     AVKGTLMKMDGEFYQIRNTDGELVRVHVDKSTKLDKVVEGDMVKAYVTDKGHVTTLQR
                     IEK"
     gene            11929..12300
                     /locus_tag="PK589_16555"
     CDS             11929..12300
                     /locus_tag="PK589_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21275.1"
                     /translation="MARSQAMDEWKGALPDSRHGGFQILDKESKEPILYHPIPGAKNT
                     GRMVREQNAGRDGLLKNRPRSICAGSYTQKKAPSSFEDGASKNLQFLYQAFITFPSCV
                     HNISIANSYDVTLSHLHSCGS"
     gene            complement(12351..>12824)
                     /locus_tag="PK589_16560"
     CDS             complement(12351..>12824)
                     /locus_tag="PK589_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21276.1"
                     /translation="MSGPGLPPCDSALTPTRRHMGMHSLIRSLLCLSLSIGLVSMALA
                     GDRLRIKNIVQHPADYDAKVVTVEGRAKAVSTLPVHRGTRRCGGSAVYDSQLFALHDR
                     SGTIGISTPGTCLPNVTKPVVENEQLRVRGIVRVDRNNPKAVPVIDAETVDRVKR"
BASE COUNT         2731 a         3993 c         3604 g         2296 t
ORIGIN      
        1 gaccatctga acggcctgct gtttctcgac cgcattcagt cgctcagcac ggacctcttc
       61 cgccggaaaa cgcagagctg agaagcatca agccctgctc gtgcgagaag atcatcggct
      121 gggccgccac cccttgaggc gagcggccac cgcttcgcgc tggtctggtg tcagcatggc
      181 tgccaggaca tcacgccgca atttggcccg cccactgtcc tgacggctgg cgagggaata
      241 ccagaaaaag gcttccagcg tatcctgggt cacgccctgc ccctgctcgt acaacatgcc
      301 catgtcgcgt tgcgccaaca catggccctg atctgcggcc aattgcagcc aacgcacggc
      361 ctcggcttca tcggtcgtgc ccaggtttcc cgtgagcaag gcttggccca acatccattg
      421 cgcattcgca tcgccctgcc cggcgagcgc ttgtagttgg tgttccgagg cgggatcggc
      481 cagtgcaggc gacaccacgg cgaccataag gattgccgcc gtcgccagta taggtgccca
      541 ctgtctcccg tccatcatca tcaccccatc gtcgctcaca gatcggccag gatcgcggcg
      601 gcggcatggc gcccggacaa cagcgccccg tccagcgtac cggtttcgca gtagtcgccg
      661 cattgataca aagcgggccg caggcgcgga gacgcggcgc cattccgact cgcaagcaca
      721 tcgaccggag gcaacgcccg gctgatgcga tcggttcgca ggtgccgcca actgtccact
      781 gccccgccaa accattcacg caactgtgct cgtacgatct gctgctggtc tgcgtcgaca
      841 ggcgccgtct gatcagcaag gctcaccgaa acgagcgccc gtccagaagg ggcataggcc
      901 ggcgcgacct cgctcaacac acaggccgtg ccgatgagcc cttgctgttc tccgttcaac
      961 aacaaccaag gcccaggcac aggcggagtg ggtgcgtcga aatagagcgt cgtcgaccga
     1021 cggccgggtg cggctggtac cggctctcct cgcaacgtgg ccgccgtggc ataatcagtc
     1081 gccagcacaa cggcatcggc cgagagacgt tcgcccgagg cgagcacgac ctgcttgccg
     1141 tcgagttgcg tgacaggcgc acgtaggcgg atcgaaccgg ctggaagtga ggaggccaac
     1201 tgaagcgcga tcgcgcccat gccggcctgc ggcaacgcaa tcgcgccgcg cgaaaatgcc
     1261 gcccacacga gttcaaagtg ccggcagggc gtggagaggg attgatccag aaacacgccc
     1321 ccgaggaacg gccggagaaa ccgctcgcgc atgaccgcgg aaaatccata gcggtccaag
     1381 gcctctaagg tcgtacgatt tccgccgcct gcttgcgcgc agaggcgctg gttcagcgcg
     1441 tcctggcgga ggcggagaat cgtgagcttg tcacggaacg agccgatcgg actcatcaac
     1501 ccggccacca gatcctgagg gcgacggagt ggatcactga tgcgatgaaa gcggccgccg
     1561 taacgtacca gcgcgccggg atgaaaggat tgcagctgca gacttggata gtcgagcacc
     1621 ttcgcggcct cgggatagcc ggtgagcaac acctgaaagc ctcgatcgaa ttgaaagcca
     1681 tccacccgat cggtgcgaac ccggcccccc gcctcatccg atgcttcgag aacagtgcag
     1741 gccagccctc gtctgaccag gtgttgtgcg caagccagcc cggcaagacc ggcaccgatg
     1801 atgatgacac gagcgctgga agacatgagg cgccgctaca cctttttaca ggcgatcttg
     1861 aactccgcct cgccgtcact ccctggcgtg acccgcgacc attcgcgtgt ggcctttccc
     1921 gattgcatgg gacggccctt ccccatctgg ctcgcataat atttgttgtg taacgggcga
     1981 ctcttgtggc cctcgcaatc gatttgtttt ttgatccgca ccgatcgata ggtgccctgc
     2041 ggaatttgtc gatccacctt gaagtccttc aacgtccaga gtgtgaccgt tttgcccgtc
     2101 gggccgatcg tcgccatgtc tacataccca tcgtacttat gcgtgccgcc gaccttcatc
     2161 cactcagccg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgaagaggc
     2281 gagcccgcag caattcgccc aacccctcct gccgatctcg accgcgagaa acgcgtgacg
     2341 ctggaccgtc tcctctcaaa actcggcatc gccagtcgga gccaggcaca ggaatggatc
     2401 agggccggac gggtccgcat caatcagcgc ctcgtacggc agccggacac ttgggtagtc
     2461 tggcccggtg atgccgtcac gctcgacgaa cagccgcttc aacaaggcgc cccgcgattc
     2521 atcctgtttc acaaacccaa aggagtggtc accacgcacg ctgacgaaca aggccggagg
     2581 accatttttg atgtactgcc gccggagttc acccgcctgc atgccgtcgg acggctggac
     2641 caagccacca gcggcctcct cctcctcacc aatgacacgg cactctccag cttcctcacc
     2701 gacccgaaac agcggatacg acggcgttac ctggtcaccg tgcgcggcga ggtcacggac
     2761 gaccgcgcgc aggcggccat cgatggactc gaggatcagg gcgaacggct tcactgcgcg
     2821 gcgctgacga ttcaaaagag atccgggcgt gaatcgcatc tggacgtgac gctgaccgaa
     2881 gggaagaacc gggaaattcg acgcctgttc aacgcgctgg gtcacgaagt cacgaggctg
     2941 cgccgcattc aatacggccc ctttgcgctc actgatcttg aaccgggcgc ctggcgcgag
     3001 atcccgatcg atgccgccaa agccttgctg gacaagggtt taccctgagt cgacacctat
     3061 cttcttttcc tacgtgctgc gcctgctcat ccggtgaaag aacttgccaa ccaccggtgc
     3121 tgtgctaagg tcaatacagc aggcgaagcg ttcgggaaca cacagtccgc atcgtactga
     3181 acaacccact ccgctctcgt ctccgcaaca ttacaccacc gtcgctcccg accagggaag
     3241 ggccggctcc aacagcagcc ggccggaata taggagatca ggatcatgaa ggagatcatg
     3301 aagcgattcg ccaccggcct caccaccttg gcctttgccg ccgccttgct ggcgccggcg
     3361 ctgacctatg ccaacggcgc cgacaaggct ccgctggaga tcaaggtgcg cgtgagcgaa
     3421 aagggattct tcgatgagaa aggcaaaccc tatggcggaa agcggatgct gcaggtgccc
     3481 aaggacacga tggtgaaaat caccttcgtc ttcggtgaag acctcaccag tttggccgcg
     3541 ggagacacgc accagattac cgttcgcgct gaagacggct ggaagcagga aaccagccct
     3601 ctgtggatga tgaatcggga atcgacggtc agtttcctgg caggcgagaa gggccgtatg
     3661 cagtaccgcg cctactgcat cctcgactgc atcggcatgg agcacttgac gaatctnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncctc gcttcgacag caagtcactg
     3841 agcttgccgt tgatatcgtc gcccatttct ttcattttct ccggcgacgg ccgctgccct
     3901 gatccgtacg attccccgcc tttgaaatag ttcgtcatca tttcgccgat ttcgatgcgg
     3961 gcattcacaa acttctccac atcagccggt tccgtcgcca ggccggttcc cgaccacagc
     4021 agcagaccga gtgagatgcc gatgatcgat cgcgatacgt acttggtcat aatctcccct
     4081 ctgagtgata gagccatgtg aactgtcttc cggcatcata ccatcccgcc ggatgcggcg
     4141 cacgggccgc gaatcgcccg cgtgcctgcc gcactactgc gatggcggca accctttcaa
     4201 gaactccccg agcacggcgc gaaaccgatc ggggtcttcc gccatcatga aatggccggt
     4261 ctggtactcc acgacctgcg aaccggcgat ctgcgcctgg atgcccttgg caatcgccgg
     4321 tgtcgccaca tcatctttga cgccgaccat gatcaatgtc ggcacctgga tacgcgtcaa
     4381 ctggccatga acgttgaact gcgacatgga ggtcagtgcc tcccggatca ccgtgccgtt
     4441 ccaggtcgga atacggtcga gcagcggctg ataggtcttc gcctgcgtgt cggccgggaa
     4501 actctccaca atcatgtttt tcgacacctc gcgatagtca cgcggcgcgc cgatggtggg
     4561 aatgtccttg tccagaatca tcggcccgtc ggtcgtggaa accaagacca acgcccgcac
     4621 acgctcagga tgatccaacg ccagccgttg cagaatcatg ccgcccatcg acacacccac
     4681 ccacaccgcc ttcttgatct ggagtacgtc ggccagtttg atcaggtcgt tggaaaactg
     4741 aggaatggaa taaccggtcg ccggcttttc cgaatcgccg tagccgcgca aatctaccgc
     4801 gatgccgcga aactccggcg aataggcccc gacatatttg gggaagatat tgctggtggt
     4861 gaccacgccg tggacgaaga tgatcggatc gcccgtcccg gcttccaaca cgctgagatg
     4921 aaggccgtga agatcgacca tatggcgttg aaacggctga tcaggcggaa tcacgtccgc
     4981 gagttggggc ggatgtgcgc cgctgttcgt gcggactgga ggggtcgtgc cggcgcagcc
     5041 gacaaacccg gccagggcca gtacgagaag gattctagaa accagcatat ggctcccctt
     5101 tctgaatagg ctgacagcta atggcttagc gtaagctgtc agccgcacag attcaatgct
     5161 ggaatcccat gagaggaggg atgtggttcg aatccccgtc cattacttga tccgggagac
     5221 cgtcacctgg atcggcacca agtcgacccg tggcggcatc gcgaacaaca cctcggcgaa
     5281 ttcgcgagag gccgcctcca gctcccgaca acgatccacg aacggactcg cggccacgcg
     5341 cgggaaatag tcgcgatggc gttcgtaaaa ctgtgcccgc cgccgccagc gatcggcatt
     5401 gacggtaaat aaccagccgg ccaggtccga gagtccccgc gacaacggct tcgcccgatc
     5461 cgacttgaaa ctgcgtgcaa ccaacgatgg agatatggtc acgtctttca tggctgcgcc
     5521 tccctcccat gcgcccgatt cgacgcccgt catcatggcg gccgggtggc gtctctgccg
     5581 gctcaccttg cctcactgta ggccggcgac atgaggaatg gattagttca gcatgaaaaa
     5641 gatttcatgg agatggcatt gagacggggg ccgggctacc gaggatctcg cggaagacgt
     5701 gagccaggtc gcgcagcgct tgcgccccgt cgcccgcata gaccgaacca tgcatggtcg
     5761 ccagagtgtt tggcttcaac gccgccaacc ggttcaaggt gggatccgtc aacgtgcagt
     5821 agggcatgta gttggccagc ggaccttgtt gatattccat gagtacctcg cgacagcgcc
     5881 cgacgacatc ggactgggtc accggttcgc catcgcccgc ctgatgaaac aggtcggaac
     5941 agagcagcgt gcgctgcgtc tcttcgaaca acaggcccga ctcccagcaa tgcggcacat
     6001 gcggcgtcgc cagaaactga aaacggtact tgcccgtctg cagcacctct ccatccgcca
     6061 ttcccttcgc cggacgaagc gccaggcaat catccacgct caccagtttt ccaaccaggc
     6121 tacacacggc gtctgactgc ggggcgaccc cctgccaatc cggcagcgac ccacattcgt
     6181 cggcttcgaa atggctgaag ctgatccagc ggagcgttcg cacgtcgatc agggaagcca
     6241 ccgcctcttt cacatcggca aacagtgcgc gcgggccggt gtgaaacaac aacggctgct
     6301 cgtcacgcac caggaattgg ctgaattgaa ggttaaacgg ttcaaggaag gtcgtgatac
     6361 gaaacaggtc cggagcgact tcagtaatct tcgccatgat gccctccagc atgaatgtgg
     6421 ctggcatcgt aacgggtgga cgggacagga tcaagatgcg cagaacgcgc ctaggctctg
     6481 tgagaagacg gacagattac ttgaacaggc ggggcagaca ggaaagcgac cgattgatca
     6541 cgtcgtcgga cggaccgacc agcgaggtgg cgggatagtg ccgcatctcc cagagcttga
     6601 cgagtcgcac cggcaggaaa aacaatccgc cccctagcag aaacaggacc catccagcca
     6661 caagcatctc cgtaccaccg gcgagccagc aacgcttcga cagcaggcca cagaatagcc
     6721 ccaaggcgac caggccttcg agcagccaga cgaactggct cagagtcaat cgcagaggaa
     6781 gccgccgggt cgcgctgaga tagatggcat ggggcccctt aggcaacggc gtcagtaatg
     6841 cgtgaccgat gcggtggatc agtgcgctca aggaaaagcc gatggcgcta tgaaagagaa
     6901 tacttgtcag cggctcgtga gccataactg ccgcggggcc gatcatgaat aacaggggcg
     6961 cagcgtagca gagccacgcc atcacgtcct cgtaggtgaa cgttcgcaac agatcgcggc
     7021 aaccttgcgt aaacgcatct ggcctgggag cgcgattata ttccataaca gtggtaggcg
     7081 gagcgtgacc gatagaacgg agaggtgtaa atcggtgagt tcctggcctt atctcgagca
     7141 gaatgatgga tgtatacgaa cttgctgttc aggtcgtttg tatcgtggtg ataccgaaac
     7201 gtcaaacaaa gaaatgtttt tcgtcgaaat gaaacctggc accggcacat ggtttcgatc
     7261 caaaaggccg cctgaccgac tagggagaag ccttcccggc cccagggatt ccgctagtcg
     7321 tgacttccga cgacaggcgg tagaacaaca tacaggcagg ttgatcgctc ttcatcacgg
     7381 gaggaggaca ccatgaaaca gcagatccag cgcccggttc gacgcaccga acaacagaag
     7441 cccacccaag ataaagatgg tgtctggccc ctgtcggcac gcgagttcat gaagcttccc
     7501 gtgtcgggac tgctctcgac cgggaaacgc atccggttca cgcgcctgac gcctcccgat
     7561 caagcggcct aaggcgcggg aaaggattga gaggtgcacc atgctcatcg aagacagtct
     7621 cctcacacat gacgaacgca aggccgccga ggccgccttc cgcggccttc ccctggacgc
     7681 acaatggtcc agaggggcgc agaccattta catcggcatt ctcgaagtga ctcgggggcg
     7741 cgatatcgtg ggagatgacc tgtgggaaga ggtggccgcc gccgtctgat tccagggcga
     7801 tcccgcgccg ccgagccgca ggccccgctg accatcacgc gcggggcctg cagagcctat
     7861 cgtcattcgt catatttccg agcattgccg tacgactttt ctaccggata cttcgcttcg
     7921 ttctttttca gtttcgcacg cacggcctca ttcacgtcga gaccgaggtc gtgacagaga
     7981 taggaaagca gaatcaccac atcggcaatt tcgtccgtga cgcgttcacg ggacgggccc
     8041 ccgagcagcc gcgccacttc ctcgtgtgtt ttccattgaa agacttccag cagttcactc
     8101 gcctcaaccg taatggcggc agcgagttcc ttgggcttat gaaactgctc ccagtcacgc
     8161 ttccgccgaa attccaatac ctccgccatc acctcagcgt tcaacatcac tgacgctcct
     8221 tatatgcgta acgaaaccaa tcccgtatcc ttgtctaccc gctgtcggta cgcgccgctc
     8281 ttgcgggcag tgtgcgacct tgccctgatt gtttgcaagc gccggcctgc tgcccgacag
     8341 ccgtgacaca caatccgacc gaatgattga aatcgcgcgg cgagtggcgt agagaattgg
     8401 cacgggaggt cccatcatga accgacggct gttgtccgcc ttgtctctcc tgctgatgct
     8461 cactgcgccg gtctatgccc aaagcccctg ctccgattgc ttctacgctg ccgaagaaga
     8521 ggtgcgcaaa tgcctcgata acgccatcag cgcgggcgat cggaatagct gcctggacaa
     8581 ccggcgggtg cgcctcaagg cctgtagcga caatcaatgc cagaccgtca gggaggaaac
     8641 ggcgatcacg gagacgcaat cgacagtcag ccggcctggc gtcgcgccct acacgccgac
     8701 ggaagccgaa tggctggcct tgatcacgag ggccggcctc cgacgggaag ccacaccgga
     8761 ccgcccctac tcgctcgaca ttattctcgc cgatccacag actctgcaga ttatggttcg
     8821 acatgcgtcc acaatggatc gggaacaact gcggaaagcc atcgaaggcg caagggagac
     8881 gatccggaac acggccaaaa gttatggctg ggacaagtgg gtcaaaatac gagagtcggt
     8941 ggaaatgtat gcggcaaaaa aatgaatccg gtgcggctac gcgtctgacg ggtcactcca
     9001 taatcagcat gatttgcgcg ggcgtgagca gcgcgcctgg ttgatggcgc cccagccagg
     9061 ccagggcgtc cggtccggac agtcccaaca ccgatcgggc gagcaacgtg gcaaacaggg
     9121 ccgtgcggcc gagtccggcg gaacaatgaa tcactacgtt atggccctgg acggcctctt
     9181 catggacccg cttcagcacc tcaggcaggc cgccatcgac gggaatgcca tagttgggga
     9241 tcggcagcgg caacaccgtc aggccttctt cccgatacat cgccaataga tcccgtcccg
     9301 tcttttcccg gcattcgtcc ggctcagcga gcagcaccac gccatgaatg gccgccgctc
     9361 tgaacttcgg cagcaacgtc ccttccggat catagaggcc gaatggcatg gcgctgccgt
     9421 agatgcgacc ctggagattg agcgggtatg acgtcagcgt cctcgcgtgg ctctgctctc
     9481 gtgatggatt actcagcatg gtgttccgcc cagtccttcc agtccgcgac cgtcgtcttt
     9541 agcatcgcct tggccgcttt cttcagccag gcagattttc gcgctgccag gtcgcgctga
     9601 acagccttga tctgcgcccg gtcgccgaga tgtacgttcg ccgtctccca gcccatcgca
     9661 tacagcagct tgccctcgtc ccgctttttc ggtaacgccg tcacttcaat ccgggagcaa
     9721 tagggcgaca gccgccggag caaccaggag ccgtccatcc ccacgaacgg atcgcggcaa
     9781 cgcacggact gctccagcat caattggtac agcaacatgc cgattggctt ctgccctcgc
     9841 gcccacagac cggcggacgc aatgagccgc ttggcttcgc gagcgatttt tcccccgcgc
     9901 cagtcggcca gcgccaccac acgcggacgc cccaggctgc cgagccccga tacccgccgt
     9961 cgcagaccat acgagaggcc tgcttccggc atgaccttct cgagagcgtt cgccacctcc
    10021 ggggggactg tgtctttgag catcggcaga gccgccatct ttttccagaa cttcaccgga
    10081 tcgcgcaggg aattcgtggc aatgtcccgc aaccaggcgt gctgctccga caacacaaag
    10141 ggccgtccgc cggcgcgcag gccttcgaga tagcccgtca acaccgcatc gcaggcctca
    10201 tccaacttca cggcgagatg ttcagcccga ccggcgagtt tggcgctgac gaccaaccgc
    10261 accaggtcgc tcgcataggg aaagacgcag gcttcgtcaa aatcgttgat gccccagatc
    10321 aagcggcctt cgctgtcccg ccacgtgccg aaattttcga catggagatc ggcgactgcc
    10381 cgcagcgccg gagccgcggt gagatcggca cagacttccg gccacacctg cagccaccgg
    10441 taacaggtcg cccgcagaaa cgaaaaggcg ctctgcgtca tcatccggtg cttcagacgc
    10501 agatcggccg gcacaaacgg cacctgccgc gctgaccatc gctcatactc atgcgtcgcc
    10561 tgaagaatgt tcatgggtgc tgcctgctca gtggcgctaa ggtctcacgt cttcagtctg
    10621 atcgcaagac gggaatcgct cgtaggagga cagcaggata ggaaggacgt cactggacct
    10681 gatgggatca cacagcgggt cttagagttt ggggatgtga agggtcttgc tgatcatgag
    10741 cgtaccggaa aggatgaaca gcagagacaa ggcatgcagg tcgccaggcc cgatcgtcca
    10801 cacacctccg tagagctgct cgccgagccg atcctgccac gctgcccagg ccagcactcc
    10861 ggtcaggagc gccgtggtcg ggatgggcgt gccttcgaaa taggccacct tgtcatggcc
    10921 ggcagaaagg cgttcggcgg tcacgttata gcgggccagc cggctcacgc cacagcagac
    10981 aaaatacgtc agtgcgatcc agtcccaccc tccccgcaga cctgcggcaa aggcgagcgc
    11041 ggcgggtgcc acgccgaacg agatgatgtc ggccaaggaa tccaattccc gtccaagcac
    11101 cgactgctga tgccgccacc gggcgacacg accgtcgaac caatcgaaca gcagcgcggc
    11161 cggcgacaac gccgcagcag ccaaaaagtg agccagcgaa tggctgctca tgtactgcat
    11221 cgcaaagaac acacccgcga gcccacaagc ggcattgccg agtgtgagca catcggcgag
    11281 atgaaactca cggagcatcg aaaaatgttt gggtggcaac acagtcacgg taggaaaacc
    11341 caagcgggat tccgctgcat ggtccacgtg gaaacttgcc cggccggttt tcatcatcgg
    11401 ccactcgaca ggaaaaataa tagcagatta ctcacgattg agaaacggca tgcacagcgc
    11461 ccatgggcgg caggcctgca gacctgccgc cccgaccacc agcgatgacg actacttctc
    11521 aatccgctgt agcgtggtga cgtggccttt atcggtgaca taggccttca ccatgtcgcc
    11581 ttcgaccact ttatcgagct tggtgctctt gtccacatgc acccggacga gctctccatc
    11641 cgtattcctg atctggtaaa actccccgtc catcttcatc agcgtgccct tcacggcatc
    11701 cttcgtgatc cgctcgccca gggtcggcga cgcttccttg tccttcatag gatcggccgc
    11761 aaacgcgaag cctcctccac cggtgagcaa cgcggcaacc agacacattt gaagagttcg
    11821 cttcatcata caacctccca tttcgttagc cgaatcgata gaacctgatt cagcctaacc
    11881 cgcgtctgga agccgacaca ctggggaaag cccctgtcgc agggagcaat ggcacgaagt
    11941 caggcgatgg atgaatggaa aggagcactt cccgattcac gccacggcgg ttttcaaatc
    12001 ttggacaagg agagcaaaga gccgatcctg tatcacccga tcccgggcgc gaagaatact
    12061 ggacggatgg tacgtgagca gaatgcggga cgagacgggc tgctgaaaaa tcgccctcgt
    12121 tccatctgtg caggcagtta tacacaaaaa aaagctccgt cctctttcga agacggagct
    12181 tccaagaatt tacaattttt atatcaggcg ttcataacgt ttccgtcatg cgtgcataat
    12241 atttccattg caaattctta tgatgtcacc ctatcgcatc ttcattcgtg tgggagttag
    12301 gtaaagccct agaaatgtgg caaggaggca atgttcacgg cagagcactg ttatctcttc
    12361 acacggtcga ctgtttccgc atcgatcact gggaccgcct ttggattgtt ccggtcaacc
    12421 cgcacgatcc cgcggacccg aagctgttca ttctccacga ccggcttcgt gacattgggc
    12481 agacaggtgc ctggcgtgct gatgccgatc gtgcccgatc gatcatgtag cgcaaacaac
    12541 tggctgtcgt acaccgcgct cccgccacag cggcgcgttc cgcgatgaac cggcaaggta
    12601 ctcacggcct tcgccctccc ctccacggtg accactttgg catcatagtc agcgggatgt
    12661 tgcacaatgt tcttgatgcg caagcgatcg ccggccaaag ccatcgacac cagcccaatg
    12721 ctcaacgaca agcacaacag gctacggata agcgaatgca ttcccatgtg tctacgcgtc
    12781 ggtgtcagag cactgtcgca gggtggaaga ccgggaccgc tcat
//
LOCUS       DAOQDS010000090        12733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_2301_length_12733_cov_20.868434, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000090 DAOQDS010000000
VERSION     DAOQDS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2301_length_12733_cov_20.868434"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PK589_16565"
     CDS             complement(<1..181)
                     /locus_tag="PK589_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21277.1"
                     /translation="MTGFVYLGGVSTALAESFAAPAAWSGLWPQTSSDGLRAPVVTEQ
                     RADLFRQSPVLSGRLR"
     gene            complement(645..2819)
                     /locus_tag="PK589_16570"
     CDS             complement(645..2819)
                     /locus_tag="PK589_16570"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ21278.1"
                     /translation="MSRKISFSRRQVIKALLGLGMIAGSNRPIALLARESADSPDALV
                     SPIIHASSDLEQREAWRMRLKDWREKVRQILRYDDSRYRESSSSWTSSSFSSCFLMLY
                     DQTFVDPVHSQYKVEEFLSQAQEDFGGFDSIILWHAYPRIGMDDRNQFDFYRDMPGGL
                     AGIRDVVRRCHRLGVKAFVVYKPWDVGTRRETVSDIEALAILVKETEADGVYLDTMPE
                     APEGLRALLDEIDSGLVLEGEGALPLNRVHDHLMSWAQHFDDGEIPVILRNKWFERRH
                     MMHQTARWQRDRHRQLQTAWMNGSGMVVWEVVFGSWVGWSARDRAILRTMLPIQRHFA
                     GLFSGEGWTPLVQTEAANVYATLWDAKGVRLWTLVNRQDQPISGLLLKIPAMKHAAYY
                     DVVTGEKLDPQVEKGEVLLQGKLASHGIGGFVTGAPETLGPDFEQLMMRQRNHQSSVY
                     LPADFPQRHVMLLGDALHYGGEVEPALADMVKIPSASFTMRVQLRTRECGFYGNEIEK
                     SELSSGLNKPRAFERRVTVQPYAIDLTLITNAQYEEFLRASGYTPQFKENFLKHWTGG
                     NVPAGKGDHPVVYVSLDDARAYARWSKKAIPSEEQWQYAAQGPEGLLYPWGNRMEPGR
                     CNGGEYGGTTSVRAYPTGRSPFGCYDMCGNVWEWTESERTDGRTRFCMIRGGSYYTAQ
                     GSHWYMDGGPQPADFAAKFLLIWPGLDRCATIGFRCVTPLQE"
     gene            complement(2982..4004)
                     /locus_tag="PK589_16575"
     CDS             complement(2982..4004)
                     /locus_tag="PK589_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNJ21279.1"
                     /translation="MGSVKGLVLVTGAAGFIGSHVAKRLLDRGDSVLGLDNLNDYYDV
                     RLKEARLARLQTHPQFQFVKLDVSDRPGMAALFQQHSIRRVVHLAAQAGVRYSLVNPH
                     AYTAANIEGFLNILEGCRHHKVEHLVYASTSSVYGGHTKMPFSVHDNVDHPVSLYAAT
                     KKANELMAHCYAHLYRFPITGLRFFTVYGPWGRPDMALFLFTKAILEGKPIDVFNYGK
                     MQRDFTYVDDIAEGVLRTLDRPAQVDPQWSSDQPDPGSSSAPYRLYNIGNNQPVDLLH
                     FIEVLEQTLGKKAEKNLLPLQAGDVPATYADVADLMRDTGFKPATSIETGIARFVEWY
                     REYYKV"
     gene            complement(4028..5647)
                     /locus_tag="PK589_16580"
     CDS             complement(4028..5647)
                     /locus_tag="PK589_16580"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ21280.1"
                     /translation="MGIWDTLPRDQYVGLAPWVWVQLESNQPPGPFPFIGGVAPEVVA
                     SLHEAHSLFLACVETAISDVFSRRATLGDPQTRVRLEDAYAELVNSRQQLSQHITARR
                     QADGQFHWSHPFDATKSATVVNTGLRIFNAVKRQAIPVPFERPMGPIVGRLLGMLDGT
                     QQAGAIQTIVATAGREGERLLTKVMELLVQYECLTPAKNTSIRSQWLSQTKDRDTIHL
                     GHAALMYRRRDQFLLFDPWLLPWFAESNVPSLWVSLLPKPAAIFLTHDHDDHVDPRTL
                     LHVPKDVPIVIPSRRNRKKYFYDYLPLLRELGFSHIIELAHGETWNFEGGAVVSVPFY
                     GEDPCDLEMPRNCYLVTDRGQNVLVHADSGPTNSGRSAIKEGVIQQLVQKYGPISLVL
                     ASQQQLQEIRSYAAHAPLSHPGLWLEVGENGYLTNSYLNELCATAQARLFVSYATGGA
                     DWYPDHLSFMFSERNPARTALLTANWEQPEKLKDLLSSHDCRYHRARALDLFRTGDDG
                     MVQVSSGVEALSPMPLYRLDHGEPAFMRRGR"
     gene            complement(5653..6084)
                     /locus_tag="PK589_16585"
     CDS             complement(5653..6084)
                     /locus_tag="PK589_16585"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNJ21281.1"
                     /translation="MPTHRGLRHLALRVTDLARSRAFYERFLAMKVVWEPDPDNVYFS
                     SGSDNLALHQIAPAELAQYQPLRGQLLDHVGVIMENPAQVDDLFRDVQQDGARYGATI
                     AKPPKQHRDGSYSFYFADPDGNVIQALYEPTISRMEFRPPA"
     gene            6256..7095
                     /locus_tag="PK589_16590"
     CDS             6256..7095
                     /locus_tag="PK589_16590"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ21282.1"
                     /translation="MLARSVLALVVALIGLNGTGANAFAAEHTSPPKEITGKDGAPMV
                     LIPAGSYPMGVPTGDRDGGRDEYPRHVIEIDPFYIDKYEVTNARYLEFVKATNHRVPQ
                     NPKNPTRNLWEGIGIPASLADRPVINVDWSDADAYCTWAGRRLPREAEWEKAAKGNND
                     WRFPWGNVEPTDGHLNFNQKWVGEKTLMPVGSYEKGKSPYGVYDMAGNVWEWVNDWYD
                     PKYYEKSPSKNPPGPETGTKKVIRGAGWQNETPTVRIFTRVDSDPTIRNESTGFRCAA
                     DAK"
     gene            7125..7967
                     /locus_tag="PK589_16595"
     CDS             7125..7967
                     /locus_tag="PK589_16595"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNJ21283.1"
                     /translation="MATAGFNGLKVVSFESRMATEMTRLIERHGGKPLVAPALREIPL
                     ADNSAALQFGEKLLREGLDVLVLMTGAGTTALFEVLETRHTKSELAAALKHTVLIARG
                     PKPVAALKSLGFQPTLTVPEPNTWMDVVSTLDAHRPVNGLRVAVQEYGVSNRDLLEAL
                     KQRGAQITPVPIYRWALPEDTAPMKEALRQILAGQVQVVLITNAAQFDHLIQLAEQEG
                     ETAMFKQACGRLVLASIGPTASERIRSHGLTVDFEPSHGKMGILVKETSEQALRLLAT
                     KRAS"
     gene            complement(8026..8946)
                     /locus_tag="PK589_16600"
     CDS             complement(8026..8946)
                     /locus_tag="PK589_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ21284.1"
                     /translation="MRGIAMWAASAAVLAMSGTLYALDVADVVREWTPEGKKLAAERV
                     KLPAHDEMIRIPAGGFLMGSDRKVDKNSYLAEFPQRKVYLDAYDIDKYEVTTVQFLKY
                     VLAHNLDPLIDWQYDGGNFQDTMVSHPVMHVSWFDAEAYCKWVGKRLPTEAEWEKAAR
                     GEDGRIYPWGNQMAGLSRANFGRTGLSGPVRDRPERLLLYPPIISVDKYDNAISPYGV
                     FQMAGNVAEWVNDWYDPKYYASAPDKNPKGPETGSQRSFRGGGWIDSTPSVRVAQRNG
                     TDPNTKMNWMGFRCARDAKEGVAGSTTEAK"
     gene            complement(8981..9640)
                     /locus_tag="PK589_16605"
     CDS             complement(8981..9640)
                     /locus_tag="PK589_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21285.1"
                     /translation="MTMCIKQTGKFDRVIGKIRRMCLWKLAGAALALTATIGIMDAAA
                     LEFTADQITKINGRTQKSNIFYRDNMWRIEHHTMGPVNISIVRKDKQVVWLLLSRMKH
                     FKTVPYKAEQDLKVSEHLEGEISREEIGTETREGHPTTLYEVTVKEGERTEVYYQWLA
                     TDIHFPMKLAKKDGSWIVEYQHVKFRPLIDYLFQLPLNFEPLEEFDHQPAAAEPNHQP
                     M"
     gene            complement(9923..10534)
                     /locus_tag="PK589_16610"
     CDS             complement(9923..10534)
                     /locus_tag="PK589_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNJ21286.1"
                     /translation="MTKRTGRALAMWCLGTGMLLSATFAQAEMARYDVDLDHSIVEFK
                     VAHMVVSKTTGHFKDYTGFIEMDPDAGTVKALEATIKTASVTTNHEKRDAHLRNPDFF
                     DAEKYPTITYTMKSYKKAGDGYVAVGDITLHGVTKEITLTGNFNGVAKDPWGNTRAGF
                     TAEGKLNRKDFGMVWNKALDSGGLVVGDDVFIRLDIECIKAKS"
     gene            complement(10537..11562)
                     /locus_tag="PK589_16615"
     CDS             complement(10537..11562)
                     /locus_tag="PK589_16615"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNJ21287.1"
                     /translation="MQHQLHSTCAGLILGLGVMFCVSVPLSATAGPSANQAPAAPKRW
                     AQFDLVSTEPDGSVQAGKDVVLSITLGGVPAGTESVMAMIESNGFQSQTVSLSEEPTA
                     SVYQGTVILEPNSTLKSRTTVHPKAVRVRVSFARAKVTGLEEFLKRDVYVTIGEPPAN
                     ETETEVQPGPVAESPEADAAAVQKATEQAMAVNATIAEEDLLPLPPPGESKAYWKQVS
                     DLISRNWSRQIRSIRRAPSSETVRVQFKMYASGIAQVIQLEKSSGARDVNDAGLQTII
                     HAQPFPPIPPDLGSDVVDVHVRMRTGAKVATRDVEMTVDKKPSKTAPPSAPTPKEGAA
                     ASGSTKE"
     gene            11715..>12733
                     /gene="tdh"
                     /locus_tag="PK589_16620"
     CDS             11715..>12733
                     /gene="tdh"
                     /locus_tag="PK589_16620"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNJ21288.1"
                     /translation="MRALVKTAAQPGLTYTERPDPSPGPNDAVIRVKATSLCGTDAHI
                     YNWDAWAHSRIHPPRIIGHEMCGEVVAVGSDVSLVTVGDYVAAESHLTCGACFQCRTG
                     QAHVCKNYRILGVDLDGSFADYIVLPETVLWKTSPDIPPELASLQEPLGNAVDAALVE
                     DLTGHTVLITGCGPTGLFAAAVARTAGAATIIATDVSDYRLGLAKQVGVDHVLNAKTD
                     GPEAIAAAILDITKGEGVDASLEMSGHPTALHHAFRSVKNGGRVTLFGIPTGPVTCDL
                     ANEVIFKGIRVHGITGRRLFSTWYRLAGLFKAGLNIRPVLTHTFPLKDFAQGFELIKS
                     GQCGKV"
BASE COUNT         2774 a         3818 c         3405 g         2736 t
ORIGIN      
        1 cacgtaatcg cccgctcaac acaggcgatt gccggaacag gtccgcgcgc tgttcggtga
       61 ctacaggcgc ccggaggcca tcactcgacg tctggggcca taatccagac caggccgctg
      121 gagcggcgaa agattcagcc agtgccgtgc tcacccctcc gagatacacg aatcctgtca
      181 taattaccgc acacaggctc aatgcgtgca gaactctagt cggtttcgtg aatgtcactc
      241 tactccatcc atcctgtcca acaagtcgag tcctatcgcc atcttaatac accctaccat
      301 gcactatcga tcgaatgtat gagtccaagg ccaaggcggt ccaccttttc ttctcgactg
      361 aaagcagaaa cgtatctatt gcggtttgct gaactaccgt ctggaaaagc ggaacaccgt
      421 agacactata gccaccttcc gcaatgacat tctcagtcac ctacatcttt acctcccatc
      481 tttctgggag aaacagcctg tttgccctcc caatagctct agagtcgggc gcttcaccga
      541 gtgcatcctg atgctattga gaggtaccgc gttcgcaaca agatagttcg gcgcatgcag
      601 ggagagatga gccaatgtgg tgtcggagaa gcagtggagc gcttctactc ttgcagagga
      661 gttacgcacc ggaaaccaat agttgcgcac cggtccaagc cgggccatat gagaagaaac
      721 ttcgcggcga agtcggctgg ttggggcccg ccatccatat accaatgaga cccctgtgcc
      781 gtgtagtagg agccgccacg gatcatacaa aatcgtgtac gcccatccgt acgttcgctc
      841 tctgtccatt cccacacgtt gccgcacatg tcataacacc cgaatggcga ccgtccagtg
      901 ggatatgctc gcacacttgt tgtccctcca tactctccgc cattgcacct tcctggctcc
      961 atcctattac cccatggata aagtaaccct tccggcccct gagccgcata ttgccactgt
     1021 tcttcgctgg gaattgcctt cttcgaccat cgagcatacg cacgagcatc gtccaaactg
     1081 acatagacta ccggatgatc tcctttgccg gcaggcacat tcccaccagt ccaatgttta
     1141 agaaagtttt ctttaaactg tggagtataa cccgaagccc tgagaaactc ttcgtactgc
     1201 gcattagtaa taagcgtgag atcgatggca tatggttgca ctgtaactcg tcgttcgaat
     1261 gcccgcggct tgttcaaccc actagagagt tcgctcttct caatttcatt accgtaaaag
     1321 ccgcattctc tcgtcctaag ttgcactcgc atggtgaagc tggcggatgg aatcttcacc
     1381 atatcggcaa gagcgggctc gacctcaccg ccatagtgca gcgcatcacc gagaagcatc
     1441 acatgacgct gtggaaaatc tgcaggcaag tagacactcg actgatgatt tcgctgtctc
     1501 atcatcagtt gctcaaagtc gggcccgagg gtttcaggag ctcctgtcac aaacccgccg
     1561 atcccgtgcg atgccaactt tccttgaagc aatacttctc ccttctcgac ctgaggatca
     1621 agtttttcgc ccgtcaccac atcgtaataa gctgcgtgct tcatcgctgg tatcttcaac
     1681 aacagccctg aaatcggttg gtcttgacga ttcaccaacg tccagagtct tacgcctttc
     1741 gcgtcccaga gcgtggcata cacatttgcc gcttccgttt gaacgagagg cgtccagcct
     1801 tctcctgaaa agagaccggc aaaatgccgt tggatcggta gcatggtgcg caggatcgca
     1861 cggtctcgag ccgaccaccc aacccaggaa ccaaatacaa cctcccaaac aaccatccca
     1921 ctgccattca tccatgccgt ttgcagctgg cgatgacgat cccgctgcca tcgtgccgtc
     1981 tgatgcatca tatgccgccg ctcaaaccac ttgttcctca gaataacggg aatttcgcca
     2041 tcgtcaaagt gttgcgccca agacattaga tgatcatgca cacgattaag tggcagcgcg
     2101 ccctctcctt ctaaaacgag gccagaatcg atttcatcaa gaagggcacg caaaccctca
     2161 ggagcttccg gcatagtatc gagatacact ccgtccgcct cagtttcctt caccaatata
     2221 gccaaggcct cgatgtccga aacggtctca cgcctagttc caacatccca aggtttatat
     2281 acgacgaacg ctttcacacc cagccggtga catcgcctaa ctacgtcccg aatgccagca
     2341 agccctcccg gcatatctcg ataaaaatcg aattgattgc ggtcatccat tccgattctc
     2401 ggataggcat gccacagaat aatactatcg aagccgccaa aatcctcctg tgcctgactt
     2461 aaaaactcct ccactttgta ttggctgtgc accgggtcaa caaacgtctg atcgtacagc
     2521 ataagaaagc aactggagaa actagatgag gtccatgaag aggatgactc acgataccgc
     2581 gaatcatcat atcgcaagat ttgccttacc ttttctctcc agtccttgag tctcattctc
     2641 caagcttctc gttgctcaag gtcgcttgac gcatggatga ttggcgatac gagagcgtca
     2701 ggagagtctg ccgattccct tgccaagagc gcaattgggc gatttgatcc cgcgatcatt
     2761 ccaagcccga gcaatgcctt aataacttgc cgacgggaga aggagatttt gcgactcatg
     2821 acagaagatc caagacccca agccgacata cgagtggggc tgtccggccc cacagtcaat
     2881 cgattcggga agacagccga gatctaccaa atctgtacgc aggttctagt tgtatagcta
     2941 aagggacaat cggcgagaat tgccaaacaa ctcgcctata ctcagacctt gtagtactcg
     3001 cggtaccact ccacgaatcg agcgatgccg gtttcaatcg aggtggccgg cttgaagcct
     3061 gtgtcccgca tgagatctgc cacatcggca taggtcgccg gcacatcgcc ggcctggagc
     3121 ggcaggagat ttttttcagc cttcttgccg agcgtctgct cgagcacctc gataaaatgc
     3181 agaaggtcga ccggctgatt attgccgata ttgtagagtc gatagggtgc agaactgcta
     3241 ccgggatcag gctgatcgct cgaccactgt ggatcaacct gcgcgggccg gtcgagcgtg
     3301 cgcaacacgc cttcggcgat atcgtcgaca taggtgaaat cgcgctgcat ctttccgtaa
     3361 ttgaagacgt cgatcggttt gccttcgaga atcgccttgg tgaacaaaaa cagcgccata
     3421 tcgggacggc cccaaggccc gtagactgtg aagaaccgca atccggtgat gggaaatcga
     3481 tacagatgcg cataacaatg cgccatcaac tcgttggctt ttttggtggc ggcatacagc
     3541 gacacaggat gatcgacatt gtcatgcacc gaaaacggca tcttggtatg gccaccgtag
     3601 actgaactcg tggaggcata gaccagatgc tcgaccttgt gatgccggca gccttccaga
     3661 atgttcagaa atccctcgat attcgcggcc gtataggcat ggggattcac gagcgagtag
     3721 cggacgccgg cctgcgccgc cagatgcacc acgcgacgaa tcgagtgctg ttggaagagg
     3781 gccgccatgc cgggacggtc tgacacatcc agctttacga attgaaactg aggatgcgtc
     3841 tgcagccgag ccaggcgggc ttctttcaaa cgaacgtcgt aataatcgtt caggttgtcc
     3901 aaccccagaa cggaatcccc gcgatccagc aatcgcttgg ccacatgcga cccgatgaat
     3961 cccgcagcgc cggtgacgag caccaatccc tttacagacc ccataacgac gcgtcccttc
     4021 tgatcaatta tcttccccgc ctcatgaatg ccggttcgcc atgatcgagg cgatagagcg
     4081 gcatcggcga caaggcctcc actccggacg agacctggac catgccatca tcacccgtgc
     4141 ggaacagatc caacgcccga gcgcgatgat accggcaatc atgtgacgac agaagatctt
     4201 ttagtttttc cggctgctcc caattggccg tgagcaatgc ggtgcgcgcg ggattgcgct
     4261 cactgaacat gaaggacaga tggtccggat accaatcggc cccgccggtg gcataggaca
     4321 caaacagccg tgcctgcgca gtggcgcata actcattcag gtaactattt gtaagatagc
     4381 cgttctcacc cacctcgagc cagagtccgg gatgagacaa tggcgcatgg gcggcataac
     4441 tgcggatctc ctgcaattgt tgttgggaag ccagcacaag ggagatcgga ccatatttct
     4501 gaacgagctg ctgaatcacg ccttctttaa tggccgaccg cccgctgttt gtcggaccgc
     4561 tgtcggcatg caccagcacg ttctgcccgc gatcggtcac gagataacag tttcgaggca
     4621 tctccaaatc gcacggatct tcgccgtaaa agggaaccga gacgacggca ccgccttcaa
     4681 agttccaggt ctcgccgtga gccagttcga tgatatggct gaaccccagt tcccgaagca
     4741 atggcaggta gtcgtaaaaa tacttcttgc gattccgccg gctgggaatg acgatcggga
     4801 catccttcgg cacatgcagc agcgtcctgg gatcgacgtg gtcatcgtgg tcatgtgtca
     4861 aaaaaattgc cgccggcttc gggagcaaag atacccacaa actcggcaca ttggattccg
     4921 caaaccaggg gagcagccag ggatcgaaca gcagaaactg gtcgcgccgc cgatacatga
     4981 gcgcggcatg cccgagatga attgtgtccc ggtccttggt ttgggagagc cactgggatc
     5041 ggattgaggt gttcttggca ggcgtgaggc attcgtattg gacgagcaac tccatcacct
     5101 tggtcaacag ccgctcgccc tcccgtcccg ccgtcgcgac gatcgtctga atggcgccgg
     5161 cctgctgtgt gccatccaac attcccagca gtcttccgac gatcggtccc atgggacgct
     5221 cgaacggcac gggaatcgcc tggcgcttca cggcattgaa gatacgcagg ccggtattga
     5281 cgacggtggc ggatttcgtc gcatcgaacg gatgcgacca atgaaactgc ccatcggcct
     5341 ggcgacgcgc cgtgatgtgc tggctcagtt gttggcggga attgacgagc tccgcataag
     5401 cgtcttccaa ccgcactctg gtctgtggat caccgagcgt cgcgcggcgg gagaacacat
     5461 cgctgatggc ggtttccacg caggccagga agaggctgtg ggcttcgtgc aaactcgcca
     5521 cgacctccgg agcgaccccg ccgataaagg gaaacggtcc cggcggctgg ttactctcaa
     5581 gctgcaccca cacccaggga gccagcccga catactgatc acgcggcaat gtatcccaaa
     5641 tacccatact cgtcaggcgg gaggccggaa ctccatgcgg ctgatagtcg gttcatataa
     5701 ggcctggatg acattgccat ccggatcggc aaaatagaag gagtagctgc catcgcgatg
     5761 ttgcttcggc ggcttcgcga tggtggcccc atagcgtgcc ccgtcctgtt gcacgtcccg
     5821 gaacagatca tccacctgcg ccggattctc catgatcaca ccgacatggt cgagcagttg
     5881 tccacgcaat ggctggtatt gcgccaactc agcaggggcg atttgatgca gggccaggtt
     5941 gtcggatccc gagctgaaat acacattgtc gggatctggt tcccatacga ccttcatggc
     6001 cagaaaccgc tcatagaacg cacgggaccg agccaggtcg gtgacacgca gggccagatg
     6061 tcgtaagcct cgatgggttg gcacgccggg atctcctcgg gcgtatagta ggtggggggc
     6121 tgtcaccccg tcaatcctca ttcttttgcc acgaacgtca ttcaccccat ctttcctcca
     6181 ccagtcatgt ttgaaaaatc cggagctgtg gtacgatcgg tcatcgattc tctcgaacca
     6241 aaggagcttg cagctatgtt ggcacgatcc gtcctggcat tagtggtagc gcttatcgga
     6301 ttgaatggca cgggggctaa tgcattcgcc gcggaacaca cctccccgcc caaagaaatc
     6361 acgggaaaag acggagcgcc gatggtcttg atcccggcag ggtcctatcc catgggggtg
     6421 ccgaccggcg accgtgacgg cggacgcgat gaatatccgc gccatgtcat cgagatcgat
     6481 ccgttttaca tcgataagta tgaggtcacg aacgcgcggt atctggaatt cgtcaaggcc
     6541 acgaatcatc gcgttcccca aaaccccaag aatcccacac ggaatctctg ggaagggatc
     6601 ggcattcctg catcgctggc tgaccgtccc gtgatcaatg ttgattggtc cgatgccgac
     6661 gcctactgca catgggccgg ccgacggctg ccgcgcgaag cggagtggga aaaagccgcc
     6721 aaggggaaca acgattggcg ctttccatgg ggtaacgtgg agccgactga cggacatttg
     6781 aatttcaacc agaagtgggt cggcgaaaag accttgatgc cggtgggtag ttacgagaag
     6841 ggcaaaagtc cttatggcgt ctatgacatg gccggcaacg tctgggaatg ggtcaatgac
     6901 tggtacgatc cgaagtacta tgaaaagagt ccgagcaaga accctccggg cccggaaact
     6961 ggcacgaaaa aagtcattcg cggtgccggc tggcagaacg agacgccaac cgtccggatc
     7021 ttcacccggg tggatagcga cccgacgatt cgcaacgaat caacagggtt tcgctgcgcg
     7081 gcagacgcca agtagcagag tcggcgggca aggcagggag ggcgatggcc acggctggat
     7141 ttaacgggtt aaaggtcgtc tcatttgagt cgcgtatggc gacggaaatg acgcgcctca
     7201 tcgagcgcca tggcggcaag ccgctcgtgg cgccggcgtt gcgggaaatt ccgctggcgg
     7261 acaattcggc ggcactacaa ttcggcgaga agctactgcg tgaagggctg gacgtgctgg
     7321 tgctgatgac cggcgccggc acgacggctc tctttgaggt tctcgagact cgccacacca
     7381 aaagcgaact cgccgcagca ttgaagcaca ccgtgctgat cgcccgtggg cccaaaccgg
     7441 tggccgcgct gaagtcgctg gggttccaac ccaccttaac cgtcccggag cctaatacct
     7501 ggatggatgt cgtctcgacg ctggacgcgc atcgaccggt gaacggattg cgcgtggcgg
     7561 tacaggaata tggcgtatcg aaccgtgact tattagaggc cctgaagcaa cggggagcac
     7621 aaatcactcc ggtgcccatc tatcggtggg cactgccgga agacaccgcg ccgatgaagg
     7681 aagccttacg tcagattctg gcgggacaag tccaggtggt gttgatcacc aatgccgcgc
     7741 agtttgatca cctgatacag ctggcagaac aggagggtga aaccgcaatg ttcaagcaag
     7801 cctgcggaag gctggtgctc gcctccatcg gccctactgc cagcgagcgc atacgcagcc
     7861 atgggctcac agtcgatttc gaaccctcgc acgggaaaat gggtattctc gtgaaagaaa
     7921 cgagcgagca ggctctgcgt cttttggcta cgaagcgagc ctcctgaaca cacccgcctg
     7981 ccaatcaggc atcattctga cacctgcacg acatcccatc ggcaattatt tggcctcggt
     8041 cgtcgatcct gctacccctt ccttggcatc tcgcgcacaa cggaatccca tccagttcat
     8101 cttggtattc ggatccgtcc cattacgctg ggcgacgcgc acgctaggtg tgctatcgat
     8161 ccagcctccg ccgcgaaagc ttcgctggct gccggtttca gggcccttgg gatttttgtc
     8221 cggggcactg gcatagtatt tcgggtcgta ccaatcattg acccattccg ccacgttacc
     8281 cgccatctga aacacgccat aggggctgat cgcattgtca tacttatcaa ccgagatgat
     8341 cggtggatag agcaacagcc gttccggacg atcccgcacc ggcccggaca acccggtccg
     8401 gccgaagttc gcccgcgaga gtcccgccat ttgattgccc cagggataaa tgcgcccgtc
     8461 ctcaccgcgg gcggcctttt cccactcggc ttcggtcggc aatcgtttgc ctacccattt
     8521 gcaataggcc tcggcatcga accaggagac atgcatcacc ggatggctga ccatggtgtc
     8581 ttgaaaattc ccgccgtcgt actgccaatc gatcagcggg tcgagattgt gggccagcac
     8641 atatttgaga aactgcacgg tcgtcacttc gtatttgtcg atatcgtagg catccaaata
     8701 gactttccgt tgcggaaatt cggccaggta ggaattcttg tcgaccttgc ggtcgcttcc
     8761 cataagaaac ccgccggctg gaatacgaat catctcatcg tgtgccggca acttcacgcg
     8821 ttccgctgcc agctttttcc cttccggcgt ccattccctg acgacatcgg cgacatcgag
     8881 cgcataaaga gtcccgctca ttgccaacac ggccgcgctc gcggcccaca tggctattcc
     8941 tcgcatacgc acgattttgc tcctttatcc gttgatcatc ctacatcggc tggtgattcg
     9001 gctccgcagc agccggctgg tgatcaaatt cttccaatgg ttcgaagttg agcggtaact
     9061 gaaacagata atcgattaat ggccggaatt tgacgtgctg atactcgacg atccagctcc
     9121 cgtctttttt ggccagcttc atggggaagt ggatatcggt ggcaagccac tggtaataca
     9181 cttctgtccg ttcgccttcc ttgaccgtca cttcatacag cgtcgtcgga tgcccttcgc
     9241 gcgtttccgt tccaatttct tcacgcgaga tttcaccttc cagatgctcg gacaccttga
     9301 gatcctgctc cgccttatag ggcacggttt tgaaatgctt catacgcgac agcagcagcc
     9361 agacgacctg cttgtcttta cgcacgatgc tgatgttgac cggccccatc gtgtgatgct
     9421 cgatccgcca catgttgtcg cgatagaaga tattggattt ctgggtccgg ccattgatct
     9481 tggtgatctg atcggccgta aactctaacg ccgcggcatc catgatgccg atcgttgctg
     9541 tcagcgccag cgctgcccct gccagtttcc acaagcacat gcggcgaatc ttaccgatga
     9601 cgcgatcgaa ttttccagtc tgcttgatac acatcgtcat accctctgcg tccccttcac
     9661 gcactccgtc ctcctccgtc ctcccccgtc cgtccaagcc ctgcgcgttg aaaccggcac
     9721 accaagaatc gacagcgaac aagatgtcca ggacatcctc gctccactat gaccagctgc
     9781 tgtgtctgct gaaaccggcg tcacgagatg ggaacgggtt gcatccccag aaagggatac
     9841 tccgccagat gcgcggttgc tggctcagcc tgcccggcac agaaccgggc aggcgcgtgg
     9901 cccctgagag aggccgtgtg gcttacgatt tcgccttaat acattcgatg tccaatctga
     9961 taaaaacgtc gtctccgacg accaggccgc cgctatcgag cgccttgttc cacaccatcc
    10021 cgaaatcctt ccgattcagt ttgccttccg ccgtaaaccc ggcgcgcgtg ttcccccacg
    10081 gatccttcgc gacgccgttg aaattccccg tcagtgtgat ctccttggtc acaccgtgca
    10141 gggtgatatc gccgaccgcc acatatccgt cgccggcctt tttataactt ttcatggtgt
    10201 aggtgatagt cgggtacttt tcagcatcaa agaagtcggg gttgcgtaaa tgcgcgtcgc
    10261 gtttttcgtg attggtcgtc accgacgcgg tcttgatcgt ggcttcaagc gccttgaccg
    10321 ttccggcatc gggatccatt tcgatgaagc cggtgtagtc cttgaaatgc ccggtcgtct
    10381 tcgacaccac catgtgggca accttgaact ccacgattga gtggtcgaga tccacgtcat
    10441 accgtgccat ttctgcttgc gcgaaggttg cactcagcag cattcccgtt cccagacacc
    10501 acatggctag cgcacgaccg gtccgtttcg tcatagttac tcctttgtag aaccgcttgc
    10561 cgcggctcct tctttgggtg ttggggcgga gggtggagca gttttggacg gctttttatc
    10621 aaccgtcatt tccacatcgc gcgtggcgac tttcgccccc gttcgcatac gcacatgcac
    10681 atcgacgaca tcgctgccga gatcgggggg aatcggtgga aacggctggg catggatgat
    10741 ggtctgaagc ccggcatcgt tcacgtcacg agccccggaa cttttctcca attgaatcac
    10801 ttgtgcaatc ccgctcgcat acattttgaa ctgcacacga acggtttcac tgctgggcgc
    10861 gcgacgaata gaacggattt gccggctcca attccggctg atcagatcac tcacctgctt
    10921 ccaataggct ttcgattcgc cgggaggagg aagcggcagc agatcttctt cggcgatcgt
    10981 cgcgttaacg gccatcgcct gctccgtcgc cttttgcacg gccgccgcat cggcttccgg
    11041 actttcggcc accggtccag gttggacctc agtctcggtt tcgttggcgg gcggctctcc
    11101 gatcgtcaca tagacgtctc gtttcagaaa ctcttccagc ccggtgacct tcgcgcgggc
    11161 gaacgacacc cggactcgca cggctttcgg atgcaccgtg gtgcgactct tcaacgtgga
    11221 attgggttcc agaatcaccg tgccttgata caccgaagcc gtcggctcct cactcaagga
    11281 caccgtctgg ctttgaaatc cgttggactc gatcatggcc atgacggatt ccgttccggc
    11341 cggtacgcca cccaacgtga tgctcaacac cacatctttg ccggcctgca ccgacccatc
    11401 cggttcggta ctgaccagat caaattgggc ccagcgtttc ggggcggccg gagcctgatt
    11461 agcggacgga ccggccgtgg ctgaaagtgg cacacttacg cagaacatca ctcccagtcc
    11521 aaggatgaga cccgcgcagg tggaatgtag ctggtgctgc atgctcgcat cctacccagg
    11581 cgccctaccc agttcaaccc gttttttggg cgccctcggc tacgagctga tccggggcat
    11641 gatccgccga cgggagatcc cgacgggttt ctgcttcggc taggcatttc ttatggtctt
    11701 gctataatga gcccatgcgt gcgcttgtga aaactgccgc ccagccgggc ctgacctaca
    11761 ccgaacgtcc cgatcccagc ccagggccta acgacgcggt gatccgggtc aaagcaacat
    11821 cgctctgcgg aaccgatgcc cacatctaca actgggatgc ctgggcccac agtcggatcc
    11881 atcctccacg aatcatcggc cacgaaatgt gcggcgaggt cgtcgcagtc gggtcggatg
    11941 tgagcctggt gacggtgggt gactatgtcg cggccgaatc acacttgacc tgtggcgcct
    12001 gctttcaatg ccgtaccggg caagcgcatg tctgcaagaa ttaccgcatc ctcggcgtcg
    12061 atctggatgg gtcgtttgcc gactacatcg tcctgccgga gactgtcctc tggaaaacat
    12121 cgccggacat tccccccgaa ctggcctctt tgcaggaacc cttgggcaat gcggtcgatg
    12181 cggcgctggt cgaagatctg accggacaca ccgtcttgat tactggttgc gggccgaccg
    12241 gcctctttgc cgccgccgtc gcccgcacag caggcgccgc caccattatc gccaccgatg
    12301 tcagtgacta ccgtcttgga ttagcaaaac aggtaggggt cgaccacgtg ctgaacgcga
    12361 aaaccgacgg accggaggcc attgccgcgg ctattctcga catcaccaag ggagaaggcg
    12421 tcgatgcctc gctggagatg tcggggcacc ccaccgcgtt gcaccatgcc tttcgttccg
    12481 taaaaaacgg cggccgcgtc acgctgttcg gcattcccac cggtcccgtg acctgcgatc
    12541 tcgccaatga agtgatattc aaaggcattc gtgtacacgg catcaccggc cggcggctat
    12601 tcagcacctg gtatcggctg gcgggactct tcaaagccgg gctcaacatc cggccggtcc
    12661 tgacccatac ctttccgctg aaagactttg cccaaggatt tgaattgatc aagtccggcc
    12721 aatgcggcaa agt
//
LOCUS       DAOQDS010000091        12654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_2325_length_12654_cov_18.769506, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000091 DAOQDS010000000
VERSION     DAOQDS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12654
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2325_length_12654_cov_18.769506"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PK589_16625"
     CDS             complement(<1..395)
                     /locus_tag="PK589_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21289.1"
                     /translation="MQPKLKSKVRCTDGEVGEVRRVIMDPLSHEISHIVVGGGTAGVI
                     ERQVPMGQVLAVSDEAVTLRGSSAEFAALPAFKRDEYVTTHEVEIAHLEDRIHVTPGE
                     VLVPFPELERSVKRRTFFANFTHAIGFLIG"
     gene            complement(401..1207)
                     /locus_tag="PK589_16630"
     CDS             complement(401..1207)
                     /locus_tag="PK589_16630"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ21290.1"
                     /translation="MGNIIKKLVVGVVVGGILFGATNALGFPFVFQALFFGYAMLGAI
                     VFMILDFPTLKPFGGVKAVGALLVFYAILSVVYIAGASMWPQYDPEDEKGKIEKILKP
                     KREHYEAGKVEVLMQRAKALDEKAKELTARLQALGAAQAPADQKAGGESAPTATTAAA
                     SGDIAKLGEEQWQLQECYNCHKLNGEGGKKRGPELDNIGNLLPAAEIARKILDPKSYK
                     AEGFEQEYEKGKMPDKYKDLMEPKDVDALATWLAGYKNASVNTPKPIKMK"
     gene            complement(1249..2952)
                     /locus_tag="PK589_16635"
     CDS             complement(1249..2952)
                     /locus_tag="PK589_16635"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNJ21291.1"
                     /translation="MKIWQRCLLVMFALVAVLGGVAYAQAPSAPPVEFPYTGNRTAVW
                     IVAQLHILFAGFILGAPIFVVISEWLGYRKQDPRYDRLAKEVTKVTVILYSMTALTGG
                     LFIFVLLATYPQFTTWLINHFFLIFAVVYPLLFIGETIVLYMYFYTWDAWKGEKKGRH
                     IALGVLLNLIGSITLFVIDAPTSFMNTPVRAEGISPAEFLATASLWDKVFNYSWMPLN
                     LHRLVGNVTFGGFVAGLIAAYMFMGSKKEEDRAYYDWMGFVGNMIGVGALLFLPFMGY
                     LLAYELCDYDASICPYMMADQLSMFFEMQGAMIGLIFLASNYYIWLSMKRIEGVERVR
                     MTVVAPIVMVLLPIVMTKVLTDYPVPDATSLAFLLPLLLAPVVLGRFIPLTVSSSTVI
                     KIGFLMVVVGNAIWMTPHGFVPTGAKLVAELELPSDWNFLALMPAKNSAAFTLVFVTV
                     VNYVIYNRAVSQGTIVWGKIDFASQFVLVFLAFSAIWTMGLMGAVRSLLRKYFHTYNL
                     LPDFTAESFTPTLSYSAWWITGITIVFYAVVSFAIIVTLRPSDSKGHAHEGSPVPAGA
                     K"
     gene            complement(3018..3524)
                     /locus_tag="PK589_16640"
     CDS             complement(3018..3524)
                     /locus_tag="PK589_16640"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ21292.1"
                     /translation="MKHAGMTRLALMVGILGFGFGVVGCGGGGEGGGEGPVVPPPPAP
                     AEYADKHMPAGWWTDPKILEEGKELYIGGKNPDVNCASCHGKDGKPVKAGARDFRNGD
                     RMKMYSDSVWFWRISEGVPNTKMKPWKSKLSEEDRWKILAFERSFGLSGKGWDANKKD
                     WVPADQVK"
     gene            complement(3736..4392)
                     /locus_tag="PK589_16645"
     CDS             complement(3736..4392)
                     /locus_tag="PK589_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ21293.1"
                     /translation="MLEESASNLGRVRVEEASRKQALGEMFVRPKTSPARIERMRGRR
                     EISKNTARHVVLVWGLIATLCVPSVLFASHAADHRFTVEGFVCGKDGRPAVNVEVLVK
                     DTRITVGQTVTTDGDGYYKVTLHLHNDNVGDPLLVEAAGEQKNSKIQFDPNDLETERK
                     LRIDFGSGCERDLGPPQWLLYGLGIAAVAIVGWIGLKISRKRMREEQRRKKGQGKRQK
                     "
     gene            complement(4560..4949)
                     /locus_tag="PK589_16650"
     CDS             complement(4560..4949)
                     /locus_tag="PK589_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21294.1"
                     /translation="MTDAKYHAPEPGSLEGRVFRPTSLTDLSEAIELAFDYRGDITVD
                     LKSGGQVTGYLFNRTATGDQPTIEVFPSTSSGTMTIPYSEVMAIAFTGEDTATGKSWE
                     AWIAKKESERQKDIDRAAAEAKSRGHL"
     gene            complement(5044..6060)
                     /gene="hpnH"
                     /locus_tag="PK589_16655"
     CDS             complement(5044..6060)
                     /gene="hpnH"
                     /locus_tag="PK589_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HNJ21295.1"
                     /translation="MAVPVSQMYTVTKYVLTQKLRGVKRYPLVLMLEPLFRCNLACAG
                     CGKIQYPDHVLDKRLTPAQCWAAADECEAPIISIPGGEPLIHPEMPEIVRGLVERKKY
                     VYLCTNAILMERKLDEYPASKFLTFSVHMDGLRDEHDLAVCRDGVYDVAVKAIKAALK
                     RGHRVTTNTTLFDDANPERVRKFFDEMMGLGVEGMMISPGYSYQKAPDQQHFLKRSRT
                     TELFSKILSNRKRGWQFNQSPLFLEFLMGKRQYQCTPWGNPTYNVFGWQRPCYLLQEG
                     YASSFRELMEKTDWDSYGTGRNEKCADCMVHCGYEASAVEDTFGSLSGFTKTVKITLM
                     PNAR"
     gene            complement(6170..7396)
                     /locus_tag="PK589_16660"
     CDS             complement(6170..7396)
                     /locus_tag="PK589_16660"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNJ21296.1"
                     /translation="MDWPSDSPHALLDPPRSTVETLPADQPVTLFDYWDVLVARRKMI
                     AAFFLFGTCLTLAISLLLPKIYESTSSVLPQLESKEGGALAALLTAPGAAGMAQNLGI
                     GLPGLPTTPTDVFVSILKSRLMADEVIKKFDLMNRYHESTMVDTRKELEDHLRITVTK
                     EKVIKVSVEDEDPQVAADIANFYVANLDRLNRTVTVSKAGQNRAFLERRLAETMESMA
                     KAEEALRDFQAKNKTVAVEAQAKVMIEAAAMIQGQITAQEVQLQVMGGYLSPDNPDLA
                     RIRSNVEELKKQLAIMGSGKDARGLLSSDRLHPAMVAVPDLALQFGRLFRQVKVQETL
                     FTLLTSQHEQAKIAEARDTPTVQVLDPAVPADKRSRPRVLLNIAVAGVLALIMGTFTA
                     YFLDYRARVRRISTPA"
     gene            complement(7413..9932)
                     /locus_tag="PK589_16665"
     CDS             complement(7413..9932)
                     /locus_tag="PK589_16665"
                     /inference="COORDINATES: protein motif:HMM:NF018010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNJ21297.1"
                     /translation="MVLPPSLLAQTLTNPAIPSSPSGASASPSIGSQISPFLPFGTYG
                     LPTAPGQAIVTNPTATQPIVPQHAPCPIPTAPDLSPENTVPNLNDYWPMTANSLLPTS
                     VEQRMRQEEEERDRQLERVQAEKEKRGLELQSQVEREKKGVSQLQGLQSAMQGAVGGM
                     QGQVQNQLNQPKQPLEQKQFTAELLRHQDFSVEEAFAQFSVLQGVKSRLKQFGYDFFD
                     AHAGGFAALQDVPVGPDYVIGPQDSLAVHIWNVPDQSFNRSYIAPVERDGMVIIPQVG
                     AIPVGGQTFSQAERTIHARLSMLLKRFELHVSMARIRTMKVYVVGEVARPGAYELSAL
                     ATASNAIYAACGPSRSGSLRQVRIMRDGKTIGQLDLYEFLLQGDRRQDHRLQAGDVVL
                     VPPLGSVVAISGSVKRPAIYELKPGTRLTELLTLAGGLTPLSDRQRCHLFRQDPALQE
                     RNMIDVDLVRAFASQGAEKSRPGVEGGDPLLMDGDYIRIATLPTQVVNVVSLVGAVKS
                     PGPYEFRAGMRVKDILTPEQLTVDAYADRAEIVRTDPATYLTKVIPFSPKAVFEGRES
                     DNVALSRLDQVVISSQMRAPALVLVEGEVRRAGYFTIEIGERLSSVLKRSGGFTPNAF
                     PNGIVLVRESVKVKQQAELDRFIAAERQRLTAQSAGIAAGTAGLNVMAGASGAMAEQQ
                     VLALRLQQLEATASRIELGRVIVSLDSIERLEGTEDDIILESRDRITIPTPPQTVGII
                     GSVKSPTSVVYRPGLDLNDYLRQAGGVTEDANKREMYVMRANGTTDSSYLSAKEMRPG
                     DTIVVPQKIEARPPQLALWQTVASIIGSLALTAAGIAVVGR"
     gene            complement(10118..11659)
                     /locus_tag="PK589_16670"
     CDS             complement(10118..11659)
                     /locus_tag="PK589_16670"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HNJ21298.1"
                     /translation="MHHWGYDAIERLIALGVIDRALVGAKPFTRMQAAQYVARAIERV
                     RADEIDIDGREAIAEPLLERLLAEFRPELIRLGTIKARPEDKTGALRFGARVTSEVDA
                     SSIGGGQTIRFRENRGGEYFVNGVQNQTDVRAWAELNEWAAVMVQPKFISNRHLLGLG
                     ATNNSDNFYLREFSLKLSYANIALEAGRGTQWWGPGYHGSLLLTDHAFPMDMIKLGTE
                     RPFQLPGFLSGLGDWKVNAFLAQLERNRDFSRARVFGLRISYLPVSWLEFGLTRLTQF
                     GGRGRDQSFPETIVDTYIHPANQGSDKDVNEQGMADFRLRLPSVPYLIPFPSGLQLYG
                     EIGTEDKWSQLPIPSRAAFLGGIYIPQVFQGDTMDLRIEYADTDYGRRRHPELGGVWY
                     NNGTYTSGMRYRGFPLGHHMGTDGTDFFVRSTRYLTDTLQLGANFNLQERDRGQPVHE
                     KKREAAVDLTWWYSKDIQVMGGYTFQRLTNPGQVSAITPFAETFAAGVTAKNHLLWTA
                     VAVQF"
     gene            complement(12048..>12654)
                     /locus_tag="PK589_16675"
     CDS             complement(12048..>12654)
                     /locus_tag="PK589_16675"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ21299.1"
                     /translation="YADQIIGGDLTSRLLRQARGRLPGTTPLVQLQAEALPLRSASRD
                     LIVCYEALYFFQDAGKFLAECRRVLAPQGQLLLCSVNPEWPEFNPNAQGRRYYCAREL
                     SALLREAGFQPDLFGAFPVGSTSISARCLSWIKRAAVQLRLIPSSMAGKRILKRLFLG
                     ELVPFPAEVDDEMAGYVPPVSVSPEHPVTGYKILFAVCQVA"
BASE COUNT         2916 a         3851 c         3289 g         2598 t
ORIGIN      
        1 ccaatcagaa aaccgatggc atgggtgaaa ttggcaaaaa atgtccgccg cttgacactg
       61 cgttcgagtt caggaaacgg caccaagact tcgcccggcg tgacatggat ccggtcttca
      121 aggtgggcaa tttccacctc gtgcgtcgtc acgtactcat cgcgcttgaa ggccggcaat
      181 gcggcaaact cggccgagct gccgcggagc gtgacggcct catcactgac tgccagcacc
      241 tgacccatcg gaacctggcg ctcgatgacg cctgccgtcc ccccgcccac gacgatatga
      301 ctgatttcgt gggagagcgg gtccatgatg accctgcgca cctctccgac ttcaccgtcc
      361 gtacatcgaa cttttgattt gagtttcggc tgcatgatgc tcacttcatc ttgatcggct
      421 tcggggtatt caccgaagcg ttcttgtatc cggccaacca ggtcgccaat gcatccacgt
      481 ccttcggttc catcaggtcc ttatacttgt cgggcatctt gcccttctcg tattcctgct
      541 cgaacccttc cgccttatag ctcttcggat cgagaatctt tctggcgatt tctgccgccg
      601 gcagaaggtt gccgatgttg tccagctccg gaccgcgttt ctttccacct tcgccattca
      661 acttgtggca gttgtaacat tcctgcagct gccattgctc ttcacccaac ttggcgatat
      721 caccactggc ggctgccgtg gtggcggtcg gcgcgctttc gccgccggcc ttttggtcgg
      781 caggcgcttg agctgcccct aatgcctgca gacgagcggt cagctccttg gccttttcat
      841 ccagagcctt ggcccgctgc atcaacactt cgaccttacc ggcctcgtag tgctcgcgct
      901 tcggcttgag aattttttca attttgccct tttcatcttc cgggtcatat tgcggccaca
      961 tggaggcgcc cgcaatgtag accacggaga gaatggcata aaacacgagc aatgccccga
     1021 ctgccttgac tccgccgaat ggcttgagcg tcggaaaatc gaggatcata aagacaatgg
     1081 cgccaagcat ggcgtagccg aaaaacagcg cctgaaagac gaacggaaag cccagcgcgt
     1141 tggtggcgcc gaacagaatt ccgccgacaa cgactcctac gaccaacttt ttaataatgt
     1201 ttcccatgaa tgccctcgtt taccgccctt cccagtatgg tgaccgccct acttggcccc
     1261 tgccggaacc gggctcccct cgtgcgcgtg accctttgaa tctgacgggc gcaaggtcac
     1321 gataatcgcg aagctgacca ctgcataaaa gacgatcgta ataccggtga tccaccacgc
     1381 ggaataagac agcgttggcg taaaggattc cgccgtgaaa tccggcaaca ggttgtacgt
     1441 atggaagtac ttccgcaaca gggagcgcac ggcgcccatc aggcccatgg tccaaatcgc
     1501 gctgaatgcg aggaagacca ggacgaattg cgaagcgaag tcgatcttgc cccacacgat
     1561 ggttccctga gaaaccgccc ggttataaat gacatagttc acaacggtga cgaagaccaa
     1621 cgtgaacgcc gcggaatttt tcgccggcat gagcgccagg aagttccagt cagacggcag
     1681 ctccaattcc gccaccagct tggcgccggt gggtacgaat ccgtggggcg tcatccagat
     1741 agcgttaccg acaacgacca tcaagaaacc gatcttaatg acagtgctcg acgaaactgt
     1801 gagcgggata aatcgcccca gaacaaccgg cgccagcaga agcggcagca ggaaggccag
     1861 cgaggtggca tccggcacgg gatagtcagt caggaccttc gtcatgacaa tgggcagtaa
     1921 caccataaca atcggcgcca ccacagtcat acgaacccgc tccacaccct cgatccgctt
     1981 catactcaac cagatgtagt aattactggc gaggaagatg agaccgatca tggccccttg
     2041 catctcgaag aacatcgaca gctgatcagc catcatgtac ggacagatgg acgcgtcgta
     2101 gtcgcagagc tcataggcca gcaagtaccc catgaacggc aagaacaaca acgcacccac
     2161 accgatcatg ttgccgacaa agcccatcca gtcgtaatag gcacgatcct cttccttctt
     2221 ggaccccatg aacatgtagg cggcaatgag accagccacg aatccaccga aggtgacgtt
     2281 tccgacgagg cggtgcaaat tgagcggcat ccagctgtag ttgaacacct tgtcccagag
     2341 agaggcagtc gccaaaaact cggctggcga aataccttca gcccggaccg gagtgttcat
     2401 gaacgaggtc ggcgcatcga tcacaaacag ggtgatcgag ccgatgagat tgagcagcac
     2461 gcccaatgca atatggcggc ccttcttttc acccttccag gcatcccatg tgtagaagta
     2521 catgtagaga acgatcgttt caccgatgaa caagagcgga tagacgacgg cgaaaatcag
     2581 gaagaagtga ttgatgagcc aggtcgtgaa ctgcggatag gtcgcaagca gcacgaagat
     2641 aaagagtccg ccggtcaagg ccgtcatgct gtacaggatg accgtgacct tcgtgacctc
     2701 tttcgccaac cggtcgtagc gtggatcctg cttgcgatat cccagccact ccgaaattac
     2761 gacgaaaatc ggagcgccca aaataaaccc ggcaaaaaga atgtggagtt gagcgacgat
     2821 ccataccgcc gtgcgattcc cggtgtaagg gaactccacg gggggcgcac tgggagcctg
     2881 ggcataggcc actcctccca ataccgccac tagcgcgaac ataaccaaca aacagcgctg
     2941 ccagattttc atgatgtctc gccgctcctc ctcattccta ccgattgacc catgactcga
     3001 actaacccag aggcgaatta cttcacctgg tcagcaggca cccaatcctt cttattggca
     3061 tcccaaccct tgccggacaa gccgaacgag cgctcaaacg ccaaaatctt ccaacgatcc
     3121 tcttcggaca acttgctctt ccacggcttc atcttcgtat tcggcacgcc ctcggagatg
     3181 cgccagaacc agacggaatc cgaatacatc ttcatacgat cgccgttacg gaagtcgcgg
     3241 gcaccggcct tgaccggctt cccatcctta ccatggcagc tcgcgcaatt gacgtcagga
     3301 ttctttccgc caatgtacaa ctccttgccc tcttcaagga tcttcggatc cgtccaccag
     3361 ccggcaggca tgtgcttgtc ggcatactcg gcaggagcag ggggtggcgg aacaaccgga
     3421 ccctcgccgc cgccttcacc gccgccaccg cacccgacaa cgccaaaacc aaacccaaga
     3481 atgccgacca tcagagccaa tctcgtcatg cctgcgtgtt tcataaaaaa gttcccgctc
     3541 ctttcttcat gtctgcgccg cttcacgcgc agacacctat tgccatcgac agaaaaaaaa
     3601 gacgcttccc tgcggtgcca cgagcagcca cagcaaaagc gtcaagactc acaatacaac
     3661 gaggtcacgt ttggacttcc ccgcctactt cttgctattc gatccggcga ggaacaatgc
     3721 cccacggacc gtgccctatt tctgccgctt gccctgtccc ttctttcgcc gctgctcttc
     3781 tcgcatccgc ttgcgggaga tctttagccc aatccatccc acaatagcaa cagccgcgat
     3841 ccccagcccg tacaacaacc actgcggagg accaagatcg cgctcgcacc cactaccgaa
     3901 atcgattcga agcttacgtt cagtctccag atcgttcggg tcgaactgga ttttcgaatt
     3961 cttctgctcc ccggccgctt cgactaacag cgggtcgccg acattatcat tatgcagatg
     4021 cagagtgact ttatagtacc cgtcgccatc agtcgtcacg gtctggccga cggtgatccg
     4081 cgtgtccttg accagcacct cgacattgac agcgggccgg ccgtctttgc cgcacacaaa
     4141 cccttcgacg gtgaatcgat ggtcggcggc gtgactcgca aacaggaccg acggcacgca
     4201 gagcgtcgcg atcaatcccc aaacaagcac gacatgccgg gcggtattct tactaatctc
     4261 tctccgcccc cgcatacgtt cgatccgtgc cggactggtt ttcggcctca caaacatctc
     4321 gcccagagcc tgtttacgag aggcttcctc gacacggact cgccccaaat ttgaggccga
     4381 ctcttctagc atagcccccc ctctttgtca acaaatggat ggagtcaaac ggagggatca
     4441 acgaggaaat acgcaccgga aattgcggct gcgcgtgaag aaaagtcgcg cgactgacgc
     4501 agaggagaac ggggacggca caccacgaga aatgcgtagc ggatcaagaa gacaagcgct
     4561 cagagatgac cgcgcgattt ggcttccgcg gcagcccgat cgatatcctt ctgtcgctcc
     4621 gattccttct tggcaatcca agcttcccag gattttcctg tggccgtgtc ttcccctgtg
     4681 aaggcaatag ccatcacctc tgaataggga atggtcatgg tcccactgct ggtagaaggg
     4741 aatacttcga tcgtcggctg atctcccgtc gcggttcgat taaagaggta gccggtgacc
     4801 tgcccgccgg atttcaggtc gaccgtgata tctccgcggt aatcaaacgc cagttcgatc
     4861 gcctcgctca gatcagtgag agatgtcggc cgaaagaccc ggccttcaag gctgccgggc
     4921 tccggcgcgt ggtacttcgc gtcagtcata atcttctcgt cttacacagg gtgacggtga
     4981 agcattcggc cggctcgcga ccgaacacct caccatagcg actctcatac cgattgcgac
     5041 cggctatcga gcgttcggca tgagggtaat cttaacggtc ttcgtaaacc cggacagcga
     5101 cccgaaggtg tcctccacgg ccgacgcctc atacccgcaa tgcaccatgc aatccgcgca
     5161 cttttcattg cgcccggtgc cgtaggagtc ccaatcggtc ttctccatga gctcacggaa
     5221 acttgaagca tagccttctt gcagcaaata gcaggggcgc tgccatccaa acacgttata
     5281 cgtggggttt ccccaagggg tgcactgata ctggcgcttg cccatgagaa actcgagaaa
     5341 caagggcgac tggttgaact gccatccacg ctttcgattg ctgagaatct tggagaagag
     5401 ctcggtggtc cggctccgct tcagaaaatg ctgttgatcc ggggcttttt gatagctgta
     5461 gcccggcgag atcatcatgc cttcgacgcc cagccccatc atctcatcga aaaacttccg
     5521 tacgcgctcc gggttggcat cgtcgaacaa cgtggtgttg gtcgtcacgc gatggcctcg
     5581 cttcagcgcc gctttgatgg ccttcaccgc tacgtcgtag accccgtcgc ggcaaaccgc
     5641 cagatcgtgc tcatcccgca gaccatccat gtggacgctg aacgtcaaga attttgaggc
     5701 cggatactca tcgagcttcc gctccatcaa aatggcattg gtgcagagat acacgtactt
     5761 tttccgttcg acgagaccgc gcacgatctc cggcatttca ggatggatca gtggttcacc
     5821 gccggggata ctgatgatcg gggcttcgca ctcgtctgca gccgcccagc attgggccgg
     5881 ggttaaccgc ttatccagca catgatccgg atattgaatc ttcccgcacc ccgcacaggc
     5941 gagattacag cgaaacaggg gctcgagcat gagcacgagc ggataccgtt tgaccccccg
     6001 gagtttctgg gtcaacacgt acttggtcac tgtatacatc tgggagactg gaacagccat
     6061 cccgttctcc tattctggta aatgtgaatg tgagtaacga ctgagacttc cggccttcta
     6121 gcagagaaat cgcgcggatt ctaccattgc gactgaaaaa tcgtcaattt cacgcaggtg
     6181 ttgaaatccg gcgaacccga gcacgatagt cgaggaaata cgcggtgaac gtccccatga
     6241 tcagtgcgag caccccggcg acggctatgt tgagcaagac acgtggacga cttcgtttat
     6301 ctgccgggac agccggatcc aacacttgca ctgtaggggt atcacgagct tccgcaatct
     6361 tggcctgttc atgctgcgaa gtgagtagcg taaagagcgt ttcctgcact ttcacttgtc
     6421 ggaacaatct cccgaactgc aaagccaggt cgggtaccgc aaccatggct ggatgaagcc
     6481 gatcactgga caagagaccc cttgcatctt tccccgaacc catgatggcc agttgcttct
     6541 tgagctcttc cacattagac cggattctgg ctaaatcggg attatcgggc gagagatacc
     6601 cccccatgac ttgcaattgg acttcctgtg ccgtgatctg tccttgaatc atcgcggcag
     6661 cttcgatcat caccttcgct tgcgcctcga cggcgaccgt cttgtttttg gcctgaaagt
     6721 cccgtagcgc ctcttccgcc ttcgccatac tttccatggt ttcagccaat cgccgttcaa
     6781 ggaacgcacg gttttgcccg gccttgctga ccgtgaccgt gcggttcagc cgatcaaggt
     6841 tcgccacata aaaattggct atatccgcag ccacttgagg atcttcatcc tcaaccgaca
     6901 ccttgatcac tttctccttc gtcacagtaa tccgcagatg gtcctccaat tcctttctcg
     6961 tatcgaccat cgtcgactcg tgatatcgat tcatgagatc gaatttcttg ataacctcgt
     7021 cggccattaa acgggatttc aaaatcgaaa cgaacacatc ggtcggtgtg gtcggaagcc
     7081 cgggcaggcc aataccgaga ttctgtgcca taccggcggc acccggtgcg gtcaagaggg
     7141 ctgccaaggc cccaccctct ttggattcca attgtggcaa gaccgatgac gttgattcgt
     7201 agattttcgg gagaagaagg ctaatcgcta acgtgaggca cgtcccgaat aaaaagaacg
     7261 cggcaatcat cttacgtcgc gccaccagga catcccaata atcaaacaat gttacgggct
     7321 gatcggcagg aagggtttca accgtggagc gcggcggatc caaaagggcg tgggggctat
     7381 ccgaaggcca atccatagac ggatccgatc gactatcggc caaccacggc aatgccggcg
     7441 gcggtcagag caagactccc aatgatactg gctacggtct gccacaacgc gagctgaggg
     7501 ggacgcgcct cgattttttg tggcaccacg atggtatctc cggggcgcat ttcctttgcc
     7561 gagagatagg aggaatccgt cgtcccattg gcccgcatca catacatctc acgcttgttc
     7621 gcatcttcgg tcaccccacc agcttggcgc aaatagtcat tcagatccaa gcctggtcga
     7681 tagaccaccg aagtgggact cttgacggaa ccaataatcc caaccgtttg gggcggtgtc
     7741 ggaatggtaa ttcggtcccg ggactccaaa atgatatcgt cctccgtccc ttccagacgt
     7801 tcgatcgaat ccaaacttac gatgacacga cccaactcga tccgtgatgc ggtcgcctcc
     7861 agttgctgaa gccgcaaggc cagcacctgc tgctccgcca tggctccact ggcgcctgcc
     7921 atgacgttca acccggcggt gccggcagct attccggccg attgagccgt taatcgctgc
     7981 cgttctgccg cgataaaccg atccagctca gcctgctgct tcaccttgac cgactcccgc
     8041 accaggacaa tgccgttcgg aaacgcattg ggcgtaaagc cgccggagcg tttcagcacc
     8101 gagctcaacc gctcgccgat ctcaatggtg aagtatcccg ctcgccgaac ctctccctcc
     8161 accaacacca gcgccggggc gcgcatctgg cttgatatga ccacctggtc cagccggctc
     8221 aacgcaacat tgtcagattc ccgaccttca aagaccgctt taggactgaa gggaatcacc
     8281 ttcgtcagat atgtggccgg atcggtacga acgatttcag cacgatcggc atacgcatcc
     8341 accgtgagtt gctcgggcgt caggatatct tttacgcgca tcccggcccg aaattcataa
     8401 gggccaggac ttttcaccgc cccgacgaga ctcaccacgt tgaccacctg agtaggcaac
     8461 gtcgcgattc gaatataatc tccgtccatg agcaacggat ctccgccctc aacgccggga
     8521 cggcttttct ccgccccttg cgacgcaaag gcgcgcacca gatccacatc gatcatattt
     8581 cgttcctgca aagccggatc ttgccggaaa agatggcagc gctggcggtc agacaaaggg
     8641 gtcagcccgc ccgctagtgt cagcaattcg gtcagacgcg tacccggctt gagttcataa
     8701 atcgccggtc gtttaactga tccgctgatg gcgactaccg atccgagtgg cggaacgagc
     8761 acgacgtcgc cggcctgaag tcgatggtct tgtcgccggt ctccttggag caaaaattca
     8821 tacaagtcca actgcccgat ggttttcccg tcccgcataa ttcggacctg tcgcagagaa
     8881 ccagatcggg acggtccgca ggctgcataa atggcattcg atgccgtggc taatgcactg
     8941 agctcatagg ctcccggccg cgccacctct ccaactacat acaccttcat ggtccggatg
     9001 cgggccatcg acacgtgtaa ttcaaaacgc ttcagcagca tgctcagccg agcatgaatc
     9061 gtacgctcgg cctgtgaaaa tgtttggccg ccgaccggaa tagccccaac ttgagggatg
     9121 atcaccatcc cgtctcgctc cacaggggcg atgtagctgc gattgaaact ttgatccggc
     9181 acattccaaa tgtgcacggc cagcgaatcc tgcgggccaa tcacataatc cggcccaacg
     9241 gggacatctt gcaacgcggc aaaaccgccg gcatgcgcgt caaaaaaatc ataaccaaac
     9301 tgctttagac ggctcttgac tccctgcagg actgaaaatt gcgcaaaggc ctcttccacg
     9361 gaaaagtcct gatgccgtag caactcagca gtaaactgtt tttgctccag cggttgtttc
     9421 ggttgattga gctgattttg gacctgcccc tgcatgcccc ccaccgctcc ctgcatggcc
     9481 gattgcagtc cctgaagttg agagacgcct tttttttccc gctctacctg gctctgtaac
     9541 tccaaaccac gtttttcttt ttcggcctgc acacgctcta attgccgatc gcgctcttcc
     9601 tcttcttggc gcatgcgctg ttcaacagaa gtaggaagca aactattcgc cgtcatgggc
     9661 cagtaatcgt tcaggtttgg caccgtgttt tcgggcgaca aatcgggagc cgtcggaatc
     9721 gggcatgggg catgttgagg aacaatgggc tgggtcgccg tcggattcgt gacaatggct
     9781 tgccccggtg cggtcggcaa cccgtaagtt ccaaatggaa ggaaggggga gatttggctg
     9841 ccgattgaag gactggcgga tgcaccggag ggggaagagg gaatcgccgg attcgtgagc
     9901 gtctgcgcga gcaaggacgg tggcaggacc atacctccta ccaagaacca gatcaccgcc
     9961 ctcatccacc ggcctgtgat ttgcccacta cgaacaaggg gcacacgcca tggcattaca
    10021 ataggcatac tgtcctaacg atctcgcctg tggataacga ctgtaggttc taatccaaag
    10081 tcaccggctc agtcaatcga ggccggaacc agcgcagcta gaattgcacg gccacagcgg
    10141 tccataggag atgatttttc gctgtgacgc cggcggcgaa ggtttcagca aacggcgtaa
    10201 tggccgacac ctgtcccgga ttcgtcaaac gctgaaaggt gtaccccccc atgacctgaa
    10261 tgtctttgga ataccaccag gtgagatcga cggcagcttc acgttttttc tcatgaaccg
    10321 gctggccgcg atctcgctcc tgaaggttga agttcgcgcc cagctgcaga gtgtcggtca
    10381 ggtatctggt gctccgcaca aaaaagtccg tcccgtctgt tcccatatga tgccccaacg
    10441 gaaacccccg gtagcgcatg ccactggtat aggttccgtt gttgtaccac acgcccccca
    10501 gttcaggatg ccgccgccgg ccgtagtccg tatcggcata ttcgatgcgc aggtccatgg
    10561 tgtcgccctg aaacacctgc ggaatataga tgccgcctaa aaacgccgca cggctcggga
    10621 tcggcagttg cgaccacttg tcctccgtcc cgatctcgcc atagagctgg agacctgaag
    10681 gaaagggaat caggtaagga actgatggca gcctcagccg gaaatcggcc atgccctgtt
    10741 cattcacgtc cttgtcagac ccctgattcg ccggatggat gtacgtatcg acgatcgttt
    10801 cgggaaacga ttggtcgcgt ccgcgcccac cgaactgcgt caagcgcgtg agcccgaact
    10861 ccaaccagga gaccggcaga taactgatgc gtaatccgaa aactctcgca cgtgaaaaat
    10921 cgcgattgcg ttccagctga gccagaaagg cattgacctt ccagtcaccc aggccgctca
    10981 gaaaccctgg caattggaac ggccgctccg tgcccaattt gatcatatcc atgggaaacg
    11041 catgatccgt gagcaacaga gagccgtggt agcctggccc ccaccactgt gttcctctcc
    11101 ccgcttcgag cgcgatgttc gcgtaactca gcttgaggct gaactcgcgc agataaaagt
    11161 tgtcgctatt gttggtcgcg cccaatccca agagatggcg attgctgatg aatttcggtt
    11221 gaaccatcac ggcggcccat tcgttcaact ccgcccaggc gcgcacatcg gtttggttct
    11281 ggacaccatt gacgaaatat tccccgcccc ggttttcccg aaaccggatc gtttgcccgc
    11341 ctccaatcga tgaggcatcg acctcagagg tcacgcgggc accgaaccgg agagcaccgg
    11401 ttttgtcctc aggacgtgcc ttaatggtgc cgaggcgaat gagttccgga cggaactccg
    11461 ccagtaatcg ttccaacagc ggctcggcaa tagcctcgcg accgtcaatg tcgatttcgt
    11521 cggcacgcac tcgctctata gcgcgtgcca catactgcgc cgcctgcatg cgggtaaacg
    11581 gcttcgcgcc caccagggcc cgatcaatca cgcccagagc aatgagacgt tcaatcgcgt
    11641 catatcccca gtggtgcaat ggaagattgc tcgaggcaac agcaacgccc ccgaacacga
    11701 accaaaggag gagtcccacc gtcaggaagc gtaggaaggg ccggactttc acagggcagg
    11761 ccgaatcaca acaatagaaa gagacgcttg agcgacgcaa ggctgcaaga ccaactcatt
    11821 ggaaaaggcc gtatggccca gacaggcacc gaaaggcttg agacgccgat gaacggtcgg
    11881 cctggccgtg ggatccaaag tcggcatgac caatctatgg agagacagtt tcttgaatct
    11941 cggaattgcc aaactgggaa gatactctgt ctcgaacaac cgaatccagt cctcggcgat
    12001 actcagtcac aagtgctgtc ccaattgagc aaacggtagg gcgaaggtca ggccacttgg
    12061 cagacagcaa agaggatttt gtaaccggtc actggatgtt cgggagaaac ggaaacggga
    12121 ggaacgtatc cggccatctc atcatccacc tcagcaggaa agggaacaag ttctcccaga
    12181 aacagccgtt tcaaaatacg cttccccgcc atactcgatg gaatcagacg taattgaaca
    12241 gcggcacgct tgatccacga cagacagcgc gcagaaatcg acgtcgaccc aacagggaag
    12301 gcgccaaaga ggtccggctg gaacccggcc tctcgtagca acgccgaaag ttcccgggcg
    12361 caatagtatc ggcgaccctg agcgttcgga ttaaattctg gccattccgg attgaccgaa
    12421 cagagcagca actgcccctg aggagcaagc acccgccggc actccgccaa aaattttccg
    12481 gcgtcttgaa aaaaatagag cgcctcataa caaacgatga ggtcgcggga tgccgatcgc
    12541 agcggcaatg cttccgcctg gagctgcacc aacggcgtag tgccaggcag gcgccctcga
    12601 gcctgccgaa gcaaccggct cgtcaggtct ccgcctataa tctgatccgc atac
//
LOCUS       DAOQDS010000092        12429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_2381_length_12429_cov_22.486989, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000092 DAOQDS010000000
VERSION     DAOQDS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12429
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2381_length_12429_cov_22.486989"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PK589_16680"
     CDS             complement(<1..758)
                     /locus_tag="PK589_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HNJ21300.1"
                     /translation="MSSLGAHNSGPDSEGPDVPEPSHAEKARTLVHLQQTGGLSTLSR
                     KQPGWPFGSVMPYGLDDQGQPSFLISTMAMHTQNLLGDPRASLLVTPPESQRDPLGAA
                     RVTLMGSVTKVPKDEAAPVRERYLARHANAAYWVDFDDFAFFRMALADIYFVGGFGSM
                     GWVAPAEYLAAAVDPLAETAADLIREMNTTQQETLLLLARECGNLDAQQASITTMDRL
                     GFHLRVKTPERMQGGRFAFTNPVRTAQEARAGVA"
     gene            complement(783..950)
                     /locus_tag="PK589_16685"
     CDS             complement(783..950)
                     /locus_tag="PK589_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21301.1"
                     /translation="MLMLIHKECGGPALEESPVGEVCAVPVDRFPFSCFSCLEEIIDE
                     SELRLIEDLGI"
     gene            complement(953..1708)
                     /locus_tag="PK589_16690"
     CDS             complement(953..1708)
                     /locus_tag="PK589_16690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNJ21302.1"
                     /translation="MTIDATMFELQGTDHKRLRGNRILGKDRQILFITGFLSKRWGNK
                     SKALAQWCQDRGWGFCCFDFRGWGDSEGQWGDYRLLHWLEDAEAVTNLLADGPPVTIV
                     GNSLGGWLAWLVAQEQPAVEELILIAPAFNMMDLRAAQISAERREQWQSAGSMPWDDE
                     PLHREAPIPWHWVEDSQTLWRRRLTMPRRVKTTILHGLQDTVIKPEGSWAFVQHVLSQ
                     DPEFPVELLLKTGDHRLSSPEDLDTFRRLVVKE"
     gene            complement(1698..2408)
                     /locus_tag="PK589_16695"
     CDS             complement(1698..2408)
                     /locus_tag="PK589_16695"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ21303.1"
                     /translation="MMMAHDRSRTEYLSTQSAYDEWAEDYDDGDPTVLLDQPFLLSML
                     QPFAGCRILDLGCGTGRYLRLASHEAALVGLDLSRGMLERARRQTPPATGATWVQGSV
                     ERIPFTSESFDRIVSGLVLDHVHDLAEFFRGIGTMLRPGGRAIVTAVHPEMQRKTGPT
                     VRFTAAGREYRTEGTIHEKAAIITALQEAGLLIEQLQEPPVTERLIARREAWKDRLGC
                     PALLLLALTRAADSRHDH"
     gene            complement(2510..3223)
                     /locus_tag="PK589_16700"
     CDS             complement(2510..3223)
                     /locus_tag="PK589_16700"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21304.1"
                     /translation="MRFEKLGGLTVRLTGGTDGKGGGNGPWVMLLHGFGALGDDLVPL
                     SEYLDAPAGTRFLFPEAPIPIPMGFGDSRAWWMIDMARIQADRAAGKIRDMSGEIPRG
                     LPEARERVKALLEDVHKKLGADPARTLLGGFSQGAMLSCDALLHSTLPYAGLIQLSST
                     LVARQEWAPLLTKRKGLPLYQSHGTQDPILPFTMAERLRDEFHLAGLVVDWQPFRGGH
                     EIPEPILRRLGSFILKTLV"
     gene            complement(3270..4745)
                     /locus_tag="PK589_16705"
     CDS             complement(3270..4745)
                     /locus_tag="PK589_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNJ21305.1"
                     /translation="MTQHSLESLTFENSYARLPEVFYAKVNPTPFRAAPFLISANPAA
                     MELIDLDPQEALRPEFAGVFGGSLLVPGMEPLAMLYSGHQFGVYVPQLGDGRAILLGE
                     IVNGRGERWDLHLKGAGMTPFSRDGDGRSVLRSAIREYLCCEAMHGLGIPTTRALCLV
                     GSDDKVYREQVETGATIVRMAPSHVRFGTFEIFYYRKQHEHLQRLADYVIEMHFPDLA
                     QAADKYVRFFAAVVERTAKLIAQWQAVGWSHGVLNTDNMSILGLTLDYGPYGFMDDYD
                     PGFICNHSDYNGRYAFNQQPYIGLWNLSCLAQTLLPFAPKEDLKAALDGYQATVDRYY
                     RDHMRQKLGLREDRGEDETLLQELKSLMVGSRVDYTIFWRELGTFSSDPAAKNERLRE
                     HFLNPDRFDVWAAQYRERLHSEQSRDEERRAGMDRINPKYVLRNYLAQTAIEKAQQKD
                     YSEIDRLLNLLQDPYRDHPGMESYAAAPPNWGKHLSVSCSS"
     gene            complement(4769..5143)
                     /locus_tag="PK589_16710"
     CDS             complement(4769..5143)
                     /locus_tag="PK589_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HNJ21306.1"
                     /translation="MTVPVPSKGEKEAAVRTAIIKFEQEFLGRGPDEVRVFIVRDMLV
                     VRLKGVLTPAERQLAKTAEGVDMVKRLRQNLIAQGRERLCEQVADLIGAKITALFTDI
                     DTVVGERIFVFTLESDLEANFR"
     gene            5534..7228
                     /locus_tag="PK589_16715"
     CDS             5534..7228
                     /locus_tag="PK589_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNJ21307.1"
                     /translation="MSFLALVPLLPLLTAFIVMTGDRADQEQNARSGLFPIAASFLGS
                     LIILIVVTSDGPIMFQLYDPAAIANLAFPIGVYIDRLSAVMMVLITGVTTLIYRYSMG
                     YMYQDRGYRRYLGMLGITTSVLLCMVSSANLVMLFIFWQILSWLLFLLAHNHGHAATL
                     SGASNTFTTLRLSDAAFLAGIVLAYSLYGTFEFQTLFTRAAETPVTLSIWPELGWTMD
                     GVTAVTLLIFGGAMGKSAQFPIHTWLPRSLYAPTPIHALLHAGIINAGGFLLNRLAPL
                     YGLSPTTLHVVFVIGMLTAILGATMMLTQNDIKKTLGFSTIGQMGYMIMECGLGAFSL
                     AVFHLIAHGLFKGTVFLNCGNVIHKARQEPSFPPIDRETEESEFSNLTWSTGFFTTLL
                     LPLVILLVTHGVLRIPLLESQGTVIFLFFIWVTSSQAILSLTRIRAVASWKVSAAMLV
                     TLVIVVFTYLFAVESFTHFLYPNPEEVAFYFKAAALPGRLFDSLVMGTTLLTILSWVY
                     LYAHAHGRTITIPGWIEAFLVRLYVLFMNRLYLDQLYLNIGRVVTQVAHRLEKRLS"
     gene            7302..8594
                     /locus_tag="PK589_16720"
     CDS             7302..8594
                     /locus_tag="PK589_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21308.1"
                     /translation="MTPWLLLAVPLLGASLALLMRAALQRMKTVALTTTGLTLLAVIG
                     TSLAQNEATAAMSLLCLLPATAFLTLLGQPLHRDNSSAWVMTLILLGVALGILTSHAP
                     THELLLILLLGLVCGLLYRHQPGTAPAAWRGMASFGLGILTALLALALPAPAATIASL
                     VTCATLLPLLPLHSGFVAALSGLPGNLPALLAFILPTVGFHSLYLLLPSLSGTVLQTV
                     ALLALAGAVYGSLRALIQSHPLPRLGYASSAFFCMLWWYVADTGTAPVQALVYLSAVG
                     LTTSGLLLAWYAIRARYGDVDFRALGGLAYPMPRFSTLLALLGLAALGMPPFGVFSGF
                     LGMLLTPAFAPSGPFAIIMLVWLTASWYYTDLIQQVVFGRPRLDIRHEDLRQTEWISL
                     MLVLLLLLILGVAPSRMFESHAAAPSSTVAMKGEPWTR"
     gene            8591..11860
                     /locus_tag="PK589_16725"
     CDS             8591..11860
                     /locus_tag="PK589_16725"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HNJ21309.1"
                     /translation="MIRTDEPADLEPRRMELRGMIRLASEVIAQYWPMRTFVHHNPLH
                     SLEYLPFTETVRRGQQFLGGHGYLPGDLYRRYVKSGRILIRHIDDALAALAKDEEVDL
                     GSCRISQREVMRACLTHGLSRPAEEPLDRLMECEPNEEQVDVLAERLVALSVPTVQDQ
                     MAAAVREDVAALGRDLTLSNWCDRTLGTRIVDQINGELIKWCEAFLDEGHATWSMPGR
                     RQGLYAAWKQVAANEWATCGIADSRRKIAALPEHPEDAVLDCLEALAIPAEFRQDYLG
                     LQLAALPGWAGFIKWRAEENDYAWQQAYPVGLVKFLAVRLWYVRELVEKVCQEELGIE
                     GNYRAVLRFMDQESAGYFMRKEWVAGRLPAAYADRVARLHAQSGSQHNRAALSREWEQ
                     LTHHYQIEFGPRRERAAQRAMARRLLTLAQALDIVPTLLMDAHPTALRQLLDWMAAFP
                     ESAHGPVWLKAFEAGYQDHLFGLLRRAPTRPQPAAPDGQPPVRPQSQSVFCIDVRSEP
                     FRRHLESTGANETYGFAGFFAALIRYRAWGKEHDTEQFPVIMRAKNEVREIPRSYLDH
                     YVSKHQSRAKLVHAGHTLLHDLKENVVTPYVMVESLGWFYVLPMMGKTFLPALYKRLT
                     NWIRRLFVPPIATILTVDKLAPAETEEMVVSEQRALIWKALRDRLGLHGSRVEAEFVE
                     ALRRRALDEDAPVEPFLSEAARAVDLSNEQLTTFLDELRRHYRINRRAASRQKERITR
                     TGFTLDEQVLTLETALRMMGLVRNFARLVLFCAHGSTTENNPFESALDCGACGGNEGK
                     PNARVLAAMANRAPVRERLAKRGIIIPADTHFLAGQVDTTTDEVQLFDLEDAPPTHRK
                     DVARLYDDLREAAQLTSQERCARFPDVGAVLPLDKAAGHVAARSADWSQVRPEWGLSG
                     NTAFIIGRRELTKGLNLSGRIFLQSYDYREDPTDRWLEVLLTAPQVVAQWINMEHYFS
                     AVDNDVYGSGSKIYHNVVGRIGIMSGPWSDLRLGLAWQTVMNDELPYHEPMRLMTLVE
                     ASRARIEKLIARHEILQHFYHNEWVHLAALDPEDGTWYRYMPSGVWRRVQHPSETTQV
                     G"
     gene            11906..12247
                     /locus_tag="PK589_16730"
     CDS             11906..12247
                     /locus_tag="PK589_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HNJ21310.1"
                     /translation="MAALTLHPMKEIRVIVSGEHRPYVTELLDKVEATGYTIIGNISG
                     KGHHGVREAHFMFSEQESLVMIMAVVPEEKVEPVLAGLRPLFDRHSGVMFVSDVSVSR
                     REYFGKKSGKP"
BASE COUNT         2344 a         3895 c         3604 g         2586 t
ORIGIN      
        1 tcggcaacac cggcgcgggc ttcctgcgcg gtgcgcactg gattcgtgaa ggcgaatcgc
       61 ccaccctgca tacgctcggg tgttttcaca cgcaggtgaa accccaatcg gtccatcgtg
      121 gtgatgctcg cctgctgcgc atcgagattg ccacattcgc gggcaagcaa cagcagtgtc
      181 tcctgttgcg tggtattcat ctcgcggatg aggtccgcag cggtctctgc caaaggatcg
      241 actgctgccg ccagatactc agccggtgca acccatccca ttgaaccaaa cccaccgaca
      301 aaataaatgt ccgccagcgc catgcgaaaa aacgcgaagt catcgaagtc cacccagtag
      361 gctgcgttgg catgacgggc cagataccgt tctcgcaccg gtgccgcctc atctttaggc
      421 accttcgtga ccgatcccat gagcgtgact ctggctgcgc ccagcggatc gcgttggctc
      481 tccggcggtg tcacgagcag gctcgcgcgg ggatctccca gcagattctg cgtgtgcatc
      541 gccatggtgc tgatcagaaa acttggctgg ccctgatcgt ccaatccata cggcatcacc
      601 gacccgaacg gccagcccgg ttgcttgcgt gagagggtgg agaggccgcc cgtctgctgc
      661 agatgcacca gcgtcctggc cttttcggca tgggagggtt cgggaacgtc cggaccttct
      721 gaatccggtc cgctgttatg tgcgcctaac gatgacatga aatgtccgcc ttcctctatg
      781 ggtcagatgc ccaaatcttc tatgagccgc aattccgatt cgtcgatgat ttcttccaga
      841 cagctgaagc aggaaaacgg aaaccgatcg accggaaccg cgcagacttc gccgaccggc
      901 gattcttcca gcgccggacc accgcattct ttgtggatta acatcaacat ggttattcct
      961 tcaccaccag ccgccggaat gtgtccaaat cctccggact gctcagccga tggtcgccgg
     1021 ttttgagcag cagttcaacg ggaaactccg gatcctgcga cagcacatgc tgcacgaagg
     1081 cccaactgcc ttccggcttg atcaccgtat cctgcaggcc atgcagaatg gttgtcttga
     1141 cgcggcgcgg catggtgagg cggcggcgcc agagcgtctg gctgtcttct acccaatgcc
     1201 agggaatcgg cgcctctcga tgcaagggtt catcatccca gggcatggag ccggccgact
     1261 gccattgttc gcgccgctca gcagagattt gcgccgctcg caggtccatc atgttgaagg
     1321 ccggggcgat cagaatgagt tcctcgacgg ccggttgctc ttgcgcaacg agccacgcca
     1381 gccaacctcc gagggaattg ccgacgatcg tcactggcgg accatcggcc agcagattcg
     1441 tgacggcttc cgcatcctcg agccagtgga gcaggcgata gtctccccac tgcccttcgg
     1501 agtcacccca gccgcgaaag tcgaaacaac aaaatcccca tcctcggtcc tggcaccatt
     1561 gcgcgagtgc cttgctcttg ttgccccagc gcttcgagag aaaccccgtg atgaacaaga
     1621 tctggcggtc tttgcctaga atgcggttcc cgcgcaggcg cttgtgatcg gtaccctgta
     1681 attcaaacat ggtggcatca atggtcatga cgagaatccg ccgcacgtgt cagagccaat
     1741 aataacagtg ccggacagcc gagccgatct ttccacgcct cccggcgggc gatgagtcgc
     1801 tctgtcaccg gtggttcctg cagttgctcg atcaagagcc ctgcctcctg aagggctgtg
     1861 ataatggcgg ccttctcgtg gatggttcct tccgtacgat attcacgccc tgcggcggtg
     1921 aaccgcaccg tcggcccggt tttccgttgc atctccggat gaaccgccgt cacgatcgca
     1981 cgcccgcctg gccggagcat ggtcccgatg ccccggaaaa attccgccag gtcgtgaaca
     2041 tgatccaaca ccaaaccgga gacaatacgg tcgaagctct ctgaagtaaa cgggatgcgc
     2101 tcaacagacc cttgcaccca ggtcgctcca gtggccggtg gtgtctggcg cctggcgcgc
     2161 tccagcatgc ctcgggacaa atccaggccg accagcgcgg cttcgtgact cgccaaacga
     2221 agatagcgac cggttccgca ccccagatcc agaattcggc atcctgcaaa aggctgcagc
     2281 atggacagaa gaaatggctg atcgagaaga acggtgggat ccccgtcatc ataatcctcg
     2341 gcccattcat cgtaggccga ctgggtggag aggtactctg tcctgctgcg atcatgtgcc
     2401 atcatcatca ccatatagga cccagcggca ccctttcagc agacggctcc attcgggagg
     2461 ggttcgggat gcaatcagag acggttttcc ctagagcttg cacaatgtgt tacacgagcg
     2521 ttttcaggat gaagcttccg agacggcgca gaatgggctc tggaatttca tgccctcccc
     2581 ggaacggctg ccaatcgacg accaggcccg ccagatggaa ttcatcccgc aaccgctcgg
     2641 ccatggtgaa cggcagaatg ggatcctgcg tgccatgact ctgataaagc ggcagcccct
     2701 tccgtttcgt gagcagaggc gcccattcct gccttgccac cagcgtgctt gagagttgga
     2761 tcagcccggc gtatggaagc gtgctatgca gcagcgcatc acaggacaac atcgcgcctt
     2821 gggaaaatcc tcccagcaga gtccgcgcag gatcagcccc gagtttctta tgtacatcct
     2881 ccagcaaggc tttcacccgc tcacgggctt caggcaggcc acgcgggatt tcgcctgaca
     2941 tgtcgcggat cttccccgcc gcccgatcgg cctgaatgcg ggccatatcg atcatccacc
     3001 aggcgcgcga atcgccgaag cccatcggaa ttgggatcgg cgcttcggga aagagaaagc
     3061 gcgtgcctgc aggtgcgtcc agatattcac tgagcggaac caggtcatcg cccaatgcgc
     3121 cgaatccatg gagcaacatc acccaggggc cattgccccc gcccttgccg tctgttccgc
     3181 ctgttagacg aaccgtcagg ccgccgagtt tttcaaaacg catcacatgc cctcatcttc
     3241 ctatcagaga acgggtcgaa ccgggtcggt caggacgagc agctcacact gagatgcttg
     3301 ccccagttcg ggggcgcggc tgcgtaggat tccatgccgg gatggtctct atacggatcc
     3361 tgaagaaggt tcaggaggcg gtcgatctca gaatagtctt tctgctgcgc cttctcgatc
     3421 gccgtttgag ccagatagtt acggaggaca tacttgggat tgatgcgatc cattcctgcc
     3481 cgtcgctctt catcgcgact ctgttcgctg tgcagtctct cacgatactg tgcagcccag
     3541 acatcgaagc ggtccggatt gaggaaatgc tcccgcaggc gttcgttctt cgccgcagga
     3601 tcggatgaaa aggtgccgag ttcgcgccag aaaatcgtgt aatccacccg actccccacc
     3661 ataagcgact tcaattcctg gagcagcgtc tcatcctctc cccgatcttc tcgcaggccg
     3721 agtttctgtc gcatgtgatc ccgataatac cggtcgacgg tagcctggta gccatcgagc
     3781 gccgctttca agtcttcctt gggcgcgaac ggcaataggg tttgtgcaag gcaactcagg
     3841 ttccacagcc cgatgtaagg ctgttgatta aacgcatagc gtccattgta gtcggaatgg
     3901 ttgcagatga agcccggatc ataatcgtcc atgaagccat agggcccata gtccagggtc
     3961 agtccgagga tcgacatgtt gtcggtattc aacacgccgt gggaccagcc gaccgcctgc
     4021 cactgcgcga taagtttcgc cgtgcgctcg accacggcgg caaaaaagcg gacatatttg
     4081 tcggcagcct gcgcaagatc agggaaatgc atctcgatta cgtaatcggc caatcgctgc
     4141 aggtgctcat gctgcttccg gtaatagaaa atttcgaagg tgccgaaccg cacatgcgaa
     4201 ggcgccatgc ggacgatggt ggcgccggtt tccacctgtt cacgatagac cttgtcgtcg
     4261 ctgccgacca ggcagagcgc gcgtgtggtc gggatgccga gtccatgcat cgcttcgcag
     4321 cagagatatt cacgaatggc cgaacgaagg acggagcgtc cgtctccatc gcgggagaag
     4381 ggagtcatcc cagcgccttt caaatgcaga tcccaccgct cccctcgccc gttgacgatt
     4441 tcccccagaa gaatcgcacg gccatcgccg agctggggca cgtacacccc gaactgatgg
     4501 ccggaataca acatcgccag cggctccatc cccggcacca gcagactgcc gccgaagact
     4561 ccggcaaatt ccggtcgcag cgcttcctgc gggtcgagat cgattaattc catcgccgcg
     4621 ggatttgcgc tgatcaaaaa cggcgcggcg cgaaacggcg tcggattcac cttcgcgtag
     4681 aacacttccg gcaggcgtgc gtaactgttc tcgaacgtga gggactccag gctgtgttgc
     4741 gtcattggct tttgcaccct tcctcacctc accgaaaatt ggcctcgaga tcggattcca
     4801 gggtgaagac aaagatgcgc tcaccaacca ccgtatcgat gtcggtgaac aacgccgtga
     4861 ttttggcacc gatgagatcc gccacctgtt cacacagtcg ttcacgcccc tgagcgatca
     4921 ggttctgacg cagccgcttg accatatcga cgccttcggc cgttttggcc agttgtcgct
     4981 cagccggcgt gagcaccccc ttcagccgca cgaccagcat gtcccgcact atgaacacac
     5041 ggacctcgtc ggggccgcgc cccagaaatt cctgttcgaa tttgatgatc gccgtacgaa
     5101 cggcggcttc cttttctccc ttgctgggca ccggcacggt catcttgctc cctggtgata
     5161 cagacatgga cgaccggatt atagtcaggg ccgccgcagg actccacccc catgtgacac
     5221 cccgacaccc ggcaatccga gccgcgatca agtgtggcgc gcccatgcgg ccggttcgcc
     5281 tgcgcggact tttctcttgc aattgcgtgg gtttcacaga tacatctaac ccagttttcc
     5341 ggtggtgccg acgacaggcc gtgattcgtg cgcggcccgg acccacatcc attgatgttc
     5401 tcagacaggc agtagggtct tgccggtgtt tcctttgcgg gaaaggccgg ggacagtgca
     5461 ggccagtcgt tggttccttg tgtgaaggac cgtcggctgg cctttttctt ttcatggcca
     5521 gcaggggatt cgcatgtcct tcttagccct cgttccgctg ttgccgttac tcacggcctt
     5581 catcgtgatg accggcgatc gtgcggacca ggagcagaat gcccgatccg gcctgttccc
     5641 catcgccgcc tctttcctgg gctcgctcat cattctgatc gtcgtgacgt cggatggccc
     5701 gatcatgttc caattgtatg atccggccgc catcgccaat ctggcgttcc ccatcggcgt
     5761 ctacatcgac cggctcagcg ccgtcatgat ggtcctgatc accggtgtca ccacgctgat
     5821 ctatcgctat tcgatggggt acatgtacca ggatcgcggg taccgccggt atctgggtat
     5881 gctcggcatc accaccagcg tcctgctgtg catggtttcc agcgctaatc tggtgatgct
     5941 cttcatcttc tggcagattc tctcctggct gttatttctg ctggcgcaca atcacgggca
     6001 tgcggcgaca ttgtccggcg cgtccaatac gtttacgacg ttacgactga gcgatgcggc
     6061 ttttctcgcg ggcattgtcc tggcctactc gctgtacggc acgttcgaat ttcagacgct
     6121 gttcacacgc gctgccgaga cgccggtgac cttgtcgatc tggcctgagc tcggctggac
     6181 catggacggc gtcaccgcgg tgaccctgct catcttcggc ggcgccatgg gaaaatcggc
     6241 ccaatttccc atccatacgt ggctgccgcg gtccctgtac gcgccgactc cgatccacgc
     6301 gctgctccat gccggcatca tcaacgccgg aggattcctg ctgaaccggc tggccccgct
     6361 gtatggcctg agcccgacga cgctgcatgt cgtcttcgtg atcggcatgc tcaccgcgat
     6421 actcggcgcc accatgatgt tgacacagaa tgacatcaag aagaccctag gattttccac
     6481 catcgggcag atgggctaca tgatcatgga atgcggcctc ggagcctttt cccttgccgt
     6541 cttccatctg atcgcgcacg ggctattcaa agggacagtg ttcctgaact gcggcaacgt
     6601 catccataag gcccgccagg aaccctcgtt cccgccgatc gaccgggaga cggaggaaag
     6661 cgaattctcc aacctgacct ggtcgacggg gttcttcacg acgctgttgc tgcccctggt
     6721 cattttgttg gtcactcacg gcgtgttgag aattccgttg ctcgaatcac aagggacggt
     6781 gatcttcctg ttcttcatct gggtcacgtc ttcgcaggcg atcctctcgc tgacgcgcat
     6841 tcgcgccgtg gcctcctgga aggtatcggc cgccatgctg gtcacgctgg ttatcgtcgt
     6901 cttcacctat ctgtttgcgg tcgagagctt cacccatttt ctgtacccga atccggaaga
     6961 ggtggccttc tatttcaagg ccgccgcgct gcccggacgg cttttcgaca gcctggtgat
     7021 gggcacgacg ctgttgacga ttctgagctg ggtctatctg tatgcccatg ctcacggacg
     7081 caccatcacc atccccggat ggatcgaagc gttcctcgtg cgcctgtatg tcctgttcat
     7141 gaaccggttg tatctggatc agctgtacct caacatcggc cgcgtcgtca cacaggtcgc
     7201 gcatcgtctg gagaaacggc tatcgtgact gcattcagac tgcgcatgtc tcgccgcgct
     7261 gctgcgtccg tgatcggagt ccagccgtga tcgagagctg gatgacaccc tggctactcc
     7321 tggccgttcc gctgttaggc gcaagcctcg ccctgctgat gagagccgcc cttcagcgca
     7381 tgaagacggt ggccctgacg acgaccgggc tgactctgct tgccgtcatc ggcacctccc
     7441 tggcacagaa cgaagcgact gccgccatgt cgttgttgtg cctgctcccg gcaacggcgt
     7501 ttctgacgct gctggggcaa cccttgcacc gggataacag ttcagcctgg gtgatgactc
     7561 tgatcctgct cggtgtggca ctaggcattc tcacgagtca cgcgcccacc cacgagctct
     7621 tgctcatcct gctcctcggt ctcgtctgcg gcttgctcta tcgtcatcaa ccgggcactg
     7681 cgccggcagc ctggcgaggg atggcttcat tcggtctggg catcctcacg gccttgctgg
     7741 cgctggcgct tccagcacca gccgcaacga tcgcctcgtt ggtcacctgc gccacgctgc
     7801 tgcccctgct gcccctgcac agcggattcg tggctgcact cagcggactg ccgggcaatc
     7861 ttcccgcgtt gttggcgttt attctgccga ccgttggatt ccatagcctg tacctgttgc
     7921 tgccgtcact ctccgggaca gtactccaga cggtggccct cctggcgttg gccggcgccg
     7981 tgtatggctc gctgcgggcc ttgattcagt cgcatcccct gccgcgattg ggctatgcct
     8041 cttcggcatt cttctgcatg ctgtggtggt atgtggccga tacgggaacc gcgccggtcc
     8101 aggccctggt ctatctcagt gccgtggggc tcaccaccag cggacttctg ctggcgtggt
     8161 atgccatccg cgcacggtac ggggatgtcg atttccgggc cctcggcggg ctcgcctatc
     8221 ccatgcctcg tttcagcact ctcctggcgc tgctcggcct tgccgccctc ggcatgccgc
     8281 cgttcggggt gttttccggc ttcctcggca tgctgctcac ccccgcgttt gcgccgtccg
     8341 ggccgttcgc catcatcatg ctcgtctggc tcacggcctc gtggtactac accgatctca
     8401 tccaacaggt ggtgttcggt cggccacgcc tggacatccg gcacgaagat ttgcgacaga
     8461 cggaatggat ctccctgatg ctggtgttgc tcttgctgct gatcctgggg gtggctccat
     8521 cccggatgtt cgagtcgcac gcggctgctc cctcctccac ggttgccatg aagggggaac
     8581 cgtggacccg atgattcgca ccgacgagcc cgccgatctc gaaccgcgcc gcatggaatt
     8641 gcgcggcatg atccggcttg ccagcgaagt catcgcgcag tactggccca tgcgaacctt
     8701 cgtgcaccat aatccgctgc atagcctcga gtatctaccg tttactgaaa cggtccgccg
     8761 cgggcagcag ttccttggcg gccacggcta cctgcccggc gacctctacc gtcgatacgt
     8821 caaatcaggg cggatcctga ttcgccacat cgacgacgcg ttggccgctt tggcgaagga
     8881 tgaagaagtc gacctcgggt cctgccgtat ttcacagcgg gaagtcatgc gtgcctgcct
     8941 gactcatgga ctgtctcgcc cggccgagga acctctcgac cggctcatgg aatgcgaacc
     9001 gaacgaggaa caggtggacg tcttggccga acgcctcgtc gccttgtccg tgccgaccgt
     9061 gcaagaccag atggccgccg cggtgcgcga agatgtggcc gcgctgggac gggaccttac
     9121 cttatcgaac tggtgcgacc ggactctggg cacccggatc gtggatcaga tcaacggcga
     9181 attgatcaaa tggtgcgaag ccttcctcga cgaaggccat gccacctggt cgatgccggg
     9241 acggcgtcag gggctgtatg cagcgtggaa acaggtggcc gctaatgagt gggcgacctg
     9301 cggcattgcc gatagccgtc gcaaaattgc cgcgctgccc gaacatccgg aagacgcggt
     9361 cttggattgc ctcgaggcgt tggcgattcc ggcagaattc cggcaggact accttggcct
     9421 tcagctcgcc gcgctgcccg gttgggccgg cttcatcaaa tggcgggccg aagaaaacga
     9481 ctatgcctgg caacaggcct accctgtcgg tctggtgaaa tttctcgccg tgcgtctctg
     9541 gtatgtgcgc gaactcgtcg agaaagtctg tcaggaggaa ttaggcatcg agggaaacta
     9601 tcgcgcggtg ctgcggttta tggaccagga gtcggccgga tacttcatgc gcaaggaatg
     9661 ggtcgccggc cgcctgccgg ccgcctatgc cgatcgggtg gcacgcctgc atgcgcaatc
     9721 ggggagtcaa cacaacaggg cggctctctc ccgtgaatgg gaacaactca cgcatcatta
     9781 tcagatcgaa ttcggcccgc gtcgcgaacg ggctgcgcag cgggctatgg cgcgtcgtct
     9841 gctcacgctc gcgcaggcac tcgacattgt gccgaccctc ttgatggacg cccatccaac
     9901 cgcgcttcgc cagctcttgg attggatggc tgcctttccg gaatcggccc atggcccggt
     9961 ttggctgaag gcgtttgagg caggctatca ggatcatctg ttcggcctgc tccggcgtgc
    10021 gccaaccagg ccgcaaccgg ctgcccccga cggtcaaccg ccggtccgcc cccagtccca
    10081 atcggtcttt tgcatcgatg tgcgatccga accgttccgc cgtcatctgg agtccacagg
    10141 cgccaatgag acctacggtt tcgcaggatt tttcgccgcc ctcatccgat atcgcgcgtg
    10201 gggcaaggag catgatacgg agcagtttcc cgtgatcatg cgcgcaaaga acgaagtccg
    10261 cgaaatcccg cgcagctatt tggaccatta cgtgtccaaa caccagtcgc gggcgaagct
    10321 ggtccatgcc ggtcacacgc tcctgcacga cttgaaggaa aacgtcgtca cgccctatgt
    10381 gatggtcgaa tcgctcggct ggttttacgt cctgcccatg atgggcaaga ccttcctgcc
    10441 ggcgctgtac aaacgcctga ccaactggat ccggcggttg ttcgtgccgc cgatcgccac
    10501 cattttaacc gtcgacaagc tggcgccggc ggaaacggaa gaaatggtgg tctccgagca
    10561 acgcgccctg atctggaaag ccctgcgtga ccggctgggc ctgcacggtt cgcgagtcga
    10621 ggcggaattt gtcgaggcgt tgcgacgtcg ggccctggac gaggatgcgc cagtggaacc
    10681 attcctcagt gaggccgccc gcgcagtcga tctgtccaac gaacaactga ccaccttcct
    10741 tgatgaactg cggcggcact atcgcattaa tcgacgtgcg gcctcgcgcc agaaagaacg
    10801 catcacccgc accggtttca cactcgacga gcaggtcctg accctcgaaa cggccttgcg
    10861 catgatgggc ctggtacgaa atttcgcccg gctggtactc ttctgtgccc acggcagcac
    10921 gacggaaaac aatccgttcg agtcggcgct ggactgcggc gcctgcggcg ggaacgaggg
    10981 caaaccgaat gcccgtgtgc tggccgcgat ggccaaccgc gctccggtgc gcgaacggct
    11041 ggcgaaacgc ggcatcatta ttcctgcgga tacgcatttc cttgcggggc aggtcgatac
    11101 cacgaccgat gaggtacagc tgttcgatct cgaagacgcg cccccgaccc accgcaagga
    11161 tgtcgcccgc ctgtatgacg atctgcggga agcagcccag cttaccagcc aggagcgctg
    11221 tgccagattt cccgacgtcg gggctgtcct gcccttggac aaggcagcag gtcatgtggc
    11281 ggcacgaagc gcggactgga gtcaggtgcg ccccgagtgg ggcctgtccg ggaacaccgc
    11341 cttcatcatt ggccggcggg aactgacgaa ggggctgaac ctgtccgggc gcatctttct
    11401 gcaatcctac gattatcgcg aggacccgac cgatcgctgg ctcgaagtac tcctcaccgc
    11461 cccgcaagtg gtggcgcagt ggatcaacat ggaacattat ttctcagccg tagacaacga
    11521 cgtgtatggc agcgggagca agatttatca taacgtcgtc ggccgcatcg gaatcatgtc
    11581 aggcccctgg agcgatctcc gtctcggcct ggcctggcag accgtcatga acgatgagct
    11641 gccttatcat gagccaatgc gcctgatgac gctcgtggaa gcctcgcgcg cgaggatcga
    11701 aaaactcatt gcgcggcatg agatcctgca gcatttctat cacaatgagt gggtccacct
    11761 ggccgcgttg gatcctgaag acggcacctg gtaccgctac atgccgtcgg gcgtatggcg
    11821 aagggttcag catccgagcg agaccacaca ggtagggtaa ccgctcactg tcgcaaccga
    11881 cgatcatttt caaaggagtc gagacatggc cgcactcaca ttgcatccca tgaaagaaat
    11941 tcgtgtcatt gtgtccgggg aacatcggcc gtacgtcacg gaattgctgg acaaggtcga
    12001 agcgaccggg tacacgatca tcgggaacat ctccggtaag ggacaccatg gcgtacgcga
    12061 ggcgcacttc atgttcagtg agcaggaaag cctggtcatg atcatggcgg tggtgccgga
    12121 agaaaaggtg gaaccggtct tggccggatt gcggccgctg ttcgatcggc actcaggagt
    12181 catgttcgtg tctgatgtgt ccgtcagccg gcgggaatat ttcggaaaga agagcgggaa
    12241 gccctgaccg gtagccggga agccacggct ggcggggccg cagggcccca gactgacctt
    12301 gtcttctcat gagtgattgt ctacaatgtg cccccgttgg cctgtaagag gaaggacggc
    12361 acgtgaaggg attacggttc gaacgactcg ggcgcgatcg ccactacaac atcattttcc
    12421 acatcggca
//
LOCUS       DAOQDS010000093        11865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_2558_length_11865_cov_21.325064, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000093 DAOQDS010000000
VERSION     DAOQDS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11865
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2558_length_11865_cov_21.325064"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="PK589_16735"
     CDS             complement(<1..1259)
                     /locus_tag="PK589_16735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HNJ21311.1"
                     /translation="MMNALQLKLAGWRTIGRVGSGQSSHAAAQGEAIEFLPAILEIQD
                     APPSPIGRAILWTIMLAFASAVTWSSLSQVDIVAVAPGKIIPSGYSKTIQPFETGVIA
                     AIHVQDGQSVKRGEVLIELDATQNSADRDRASNEYRAALVEAARLHALIAGHSTFTAP
                     SESDPEFVLLQQQLLRDQMVEYAARVDAARHLIGQRKAAVDAAKDNLRRLEATVPMEN
                     ERAAAYRALLAQQYVSKMDYLQFEQQRIDKAQEWAGQKSKLRQDQAALAEAEQNYQVL
                     ISEFQQSKQAELSAMETKATSLVQEVRKAGQKTELQSLVSPIDGVVQQLAVHTVGGVV
                     TPAQTLLMVVPQDHPVEVEAQLENKDIGFVKEGQPVELKIETFPFTLYGTIPGKVLTV
                     SDDAVPLDKEKGGLVYASRVSMDRATM"
     gene            complement(1259..3421)
                     /locus_tag="PK589_16740"
     CDS             complement(1259..3421)
                     /locus_tag="PK589_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HNJ21312.1"
                     /translation="MDVPTSHLESCSTAPPRPDTGLLCLLLIARFHDLPIDSGQIQHQ
                     FGLSGHMLTTNALVRAAKHVGLKAGLVSSSWGELAGAPLPAIAQRTDGTYVVLAKVQG
                     EKALLQDPLKERPHILSKEEFVACWSGSLLLFTKRSGLRVEDVSFDMTWFIPAVLKYR
                     RLLGELVLASFFLQVFALLTPLFTQVVIDKVLVHKGFTTLHVMAVGMLALALFDALLG
                     GLRTYLFAHTTNRIDVGLGAQLFRHLLALPLAYFEARRVGDTVARVRELEQIRQFITS
                     NSVTVVLDVVFTIVFLGVMWIYSPTLTLVVMASLPLYALLSVLITPAIRARLHEKFNR
                     GAENQSFLVEAVSGIQTVKALAVEPPLQRRWDEQLAGYVAASFRATSLITVAGQIATC
                     IQKSTTIAIMWVGAYQVIDGALSIGELIAFNMLSGQVTGPLLRMVNLWQEFQQVGISI
                     QRLGDVLNAKLEPSYNPNRTTLPQIAGQIVFDDVAFRYRVDGPLVLQRVSLTLHPGQV
                     VGVVGRSGSGKSTIAKLMQRLYVPERGRVLVDGVDLAQIDPAWLRRQVGVVLQENFLF
                     NRSVRDNIALTDPGLPMDRVMHAAKLAGAHEFILELPDGYDTVVGEHGCALSGGQRQR
                     IAIARALVANPRILIFDEATSALDYESEAIIQRNMAQICKGRTVIIIAHRLSTVRPAH
                     KIYVIDRGQLVEQGTHDELLSRDGMYTRLHQHQQGRAA"
     gene            complement(3443..>3827)
                     /locus_tag="PK589_16745"
     CDS             complement(3443..>3827)
                     /locus_tag="PK589_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21313.1"
                     /translation="RGIRSSMSFICLKEKRMAVGSSPKQNGDKAVAVNVRLQSSTEAD
                     QPILANYLHASLAQGLAYIDFGFLEPSLIGAVAQRAQKGEAMPKQLVGSRAVRVALPL
                     DALIRLHQQLQQMVASLQPAKSKLS"
     assembly_gap    3828..3927
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3928..4377
                     /locus_tag="PK589_16750"
     CDS             <3928..4377
                     /locus_tag="PK589_16750"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNJ21314.1"
                     /translation="RYAGTWHEIARLPMWFQRHCVDSKATYTVRNDGAIGVRNECVTD
                     TGGHDEVEGVATVVDPGTNTRLRVVFDNFFARLFGSSREGNYWILALDADYQTAMVGT
                     PDRRYLWILSRSRQLDETAYTRLVEQARRLRFPVSELIRPHRSAATS"
     gene            complement(4469..5374)
                     /locus_tag="PK589_16755"
     CDS             complement(4469..5374)
                     /locus_tag="PK589_16755"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNJ21315.1"
                     /translation="MWALLKSLATQTIAKWSDEPGPRFAAALAFYTALTIVPLVVLTI
                     MVSVSMVGDESMVGGLQRQIGELIGESAANGLFRIIAQWRSAGSPLVNGVVALGVFVI
                     GALKVMDQLRDALDCIWGLQPKHPPRMGDRLKRRFASLSGLFGIAFILLASLTLSAWT
                     GIAIQFVLETTGMPGRLVQGIGLLMSFGVVSTLFAMIYKWVPQAQVAWSDVWVGAGIT
                     AILYLLGSKLLVVYLAHNTLVAFYGAAGSLVLVLLWAYYASQVFLFGAAFIAVYATHH
                     GSQVKPTEETVVVHVASQGESETEG"
     gene            complement(5537..5761)
                     /locus_tag="PK589_16760"
     CDS             complement(5537..5761)
                     /locus_tag="PK589_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21316.1"
                     /translation="MDKAQNLASAARANLNSEMQGYADALQRIIDLARNGAMLGHDHA
                     YDACCKIEAIATEALQRPTTPHRPDDKPTG"
     gene            complement(5922..6257)
                     /locus_tag="PK589_16765"
     CDS             complement(5922..6257)
                     /locus_tag="PK589_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21317.1"
                     /translation="MMKVPTYVSLVLCIAVLGGCNYSDGDRTGYLQKLSRKGWVCKTY
                     EGELAMTTVPGVAPVLWNFSVWDDDVAKKLDGQMGKRVVLHYREFRYVPTTCFGETSY
                     FIDRAEVSE"
     gene            6466..6924
                     /locus_tag="PK589_16770"
     CDS             6466..6924
                     /locus_tag="PK589_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21318.1"
                     /translation="MIATLATALLVVLATVPNGHAVAQSSQRDVFDERDTRVFKAVGC
                     TQDKQPAEALYYIVASRSDLSAGVPSPSAQFMKDEVEQNWQRIASQLTMEQVTEERFT
                     DTYHALLNDMIPRLQRAVEEKSGVSIAVSEVNSRPMDRTKDQDVPTCSSQ"
     gene            7192..7344
                     /locus_tag="PK589_16775"
     CDS             7192..7344
                     /locus_tag="PK589_16775"
                     /inference="COORDINATES: protein motif:HMM:NF023180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HNJ21319.1"
                     /translation="MSTILLIILILLLIGALPSWPYSSGWGYYPSGGIGLILLIVLIL
                     ALTGRV"
     gene            complement(7451..7771)
                     /locus_tag="PK589_16780"
     CDS             complement(7451..7771)
                     /locus_tag="PK589_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21320.1"
                     /translation="MSLKIDVTTVARVLLRDGSWYEVIPGSLTMDTFQWTDREGITIA
                     SVDLEALPPMGIRFKTRGMSGWLSCPLTSIVAVHYEPIELEDPLAVELPSLLSDELDR
                     PSVQ"
     gene            complement(8255..>8530)
                     /locus_tag="PK589_16785"
     CDS             complement(8255..>8530)
                     /locus_tag="PK589_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21321.1"
                     /translation="EWTAQEYMAQAMGHISQLSALVGRTLEASRTGQPQAPTDICAVM
                     KCPRLDRYEAAIEEAIQVMEHTKSSFKSKELGTLRQHLEDVLREGRR"
     assembly_gap    8531..8630
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8631..8854)
                     /locus_tag="PK589_16790"
     CDS             complement(<8631..8854)
                     /locus_tag="PK589_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ21322.1"
                     /translation="MTITPKRILIADDEETFRESTVAILADAGYACNAARDAIEAERL
                     LENGYDLLLADVRMPGNSQLEFLETVARRHP"
     gene            complement(8820..11597)
                     /locus_tag="PK589_16795"
     CDS             complement(8820..11597)
                     /locus_tag="PK589_16795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNJ21323.1"
                     /translation="MTKSAAPGLLPSNGGFALNYPDRPWYALSACILLATLLIDLYTP
                     AGIPASLLYLLAVAAVGLVSGSRMQWIVAAISTGLTVVGLIGTSPESIGWIEVTNRAL
                     SIGAAWLTAWIVSQWQKCARSRAHDVSIRLRSLLTHSQTIIFVKDLQGRYLEVSEQFL
                     TLVGVPAERVIGKTDHDLFPRDIADSYRQHDCEVVHAGGVMSFEEDALLNHDPRFYVV
                     RKFPLRDEAGRIIAVGGVATDMTARLQAEVARHDAQERLDLVVAATKTGIWDWDLQAN
                     TMYYSPRWKSSLGYEEQEISNSPSEWESRLHPDDKKRALALVDDYLSGRIASYELEHR
                     LRHKDGTYRWIHTCAVFQRDEQGRPRRMTGSHVDITERKRAEEALSRSESTLRGLFES
                     GVMLMGVVELHDGDILHLSDNRCAAGFFGTTPELMQGKWASKLGAPPDTIALWHRHYE
                     MSRESAQPVRFEYPHTCPTSGEERYLSVSVCWIGTGPSGRDRYSYVAEDVTEARRTKT
                     ALVETAERYRGVVTALAEGIVLQNAQGRIIACNPSAEQILGLTAEQMMGRTSVTSEWR
                     AIHKDGTPFEGEQHPAMVTLHTGQPCADVIMGVCRPGGEQRWLSINTQPLREPHAERP
                     YAVVASFHDITERRRAEQALVEVQTQLEQRVRERTARIHELEAQRSQAEKVAALGHLA
                     ADIAHEINNPLAGIKNAFHLVKQGVSPDHRHYRFVELIDREIQRLAAIVKKMYTLYQN
                     GASGQWQATDVSDLLQNVGTLLDYKLVSRRIQLRTEIELTCPVVTLPRADLFQVLLNL
                     VQNAIDASPQDMEVVLKVARHGDTLHWSVMDHGPGITPEVFPHIFEPFFTTKSAGGPQ
                     GLGLGLSVSRGLVRAMGGRIEVHTRPAGGSTFTVIHPLNVTDGVGQQGTGAALKKEQE
                     DDDYAKTHSHC"
BASE COUNT         2595 a         3473 c         3087 g         2510 t
ORIGIN      
        1 tgcatcgtgg ctcgatccat actcacgcgg ctcgcataga ccagaccgcc tttttcttta
       61 tctaacggta ccgcatcgtc ggagacggtc aacacctttc cgggaatcgt cccgtacaac
      121 gtaaacggaa acgtttcgat cttgagttcc accggctggc cttccttcac aaatccaatg
      181 tctttatttt cgagctgcgc ttccacttcg accgggtggt cctgcggcac aaccatcagc
      241 agagtttgag ccggcgtcac tactccgcct actgtgtgca cggccaattg ctgaacaaca
      301 ccatctatgg gagaaaccag actttgaagt tcagtttttt gtccggcttt ccgcacctct
      361 tgtacaagtg acgtggcttt cgtctccatg gccgacagtt ctgcttgctt actttgctga
      421 aattcagaaa tgagcacctg atagttctgc tctgcctcgg ccaaggccgc ttgatcctga
      481 cgtagcttgc tcttttgccc tgcccattcc tgagctttat cgatgcgttg ttgctcgaat
      541 tggagatagt ccatcttcga cacatactgt tgtgccagca atgcccggta agccgctgcc
      601 ctttcatttt ccatcggaac ggtggcttcg agacgtcgga gattgtcttt ggccgcatct
      661 acagccgctt tacgctgccc gatgagatgt cgtgcagcat ctacccgggc ggcatactcg
      721 accatctgat cccgtaaaag ctgctgttgc agcaacacaa attccgggtc tgactcactg
      781 ggcgcggtga acgtactgtg cccggctatc agcgcatgca gccgagccgc ctcgaccaga
      841 gccgctcgat attcattgga agcccgatca cggtctgcgc tgttttgagt cgcgtcaagc
      901 tcaatcagaa cttctcctcg tttgaccgat tggccgtcct gcacatgaat cgcagcaatc
      961 acacctgtct cgaacggttg aatggtttta gaatagccgc tgggaataat tttgcccggc
     1021 gccaccgcca cgatatcgac ctggctcaac gaagaccatg tcacggctga ggcgaaggcc
     1081 agcatgatgg tccagagaat cgcccggcca atcggcgacg gcggtgcatc ctgaatttca
     1141 agaattgcgg gcagaaattc gatcgcttct ccttgggctg cggcgtgaga tgactgaccg
     1201 gaaccaacgc gcccgattgt tcgccatcct gccagcttga gttgaagggc attcatcatt
     1261 acgccgcacg tccttgctga tgctgatgaa gtcgcgtata catcccgtcg cgcgagagca
     1321 attcatcatg cgtgccctgc tcaaccaatt gccctcgatc aatgacgtaa atcttgtgag
     1381 caggacgaac cgtgctgagc cgatgcgcga tgatgatgac cgtacgccct ttgcagatct
     1441 gggccatatt gcgctgaatg atcgcttcgg actcatagtc caaggcactc gtcgcttcat
     1501 cgaagatgag gatgcgcggg ttcgccacca atgcgcgtgc gatcgcaatg cgctgccgct
     1561 gtccgccgga gagcgcgcag ccatgctctc cgacgaccgt gtcgtatcca tccggtaact
     1621 caagaatgaa ctcgtgagcg cccgcaagtt tggcagcatg catgacacgg tccatgggaa
     1681 gcccaggatc ggtcaacgcg atgttatccc gcactgaccg gttgaagaga aagttctctt
     1741 gcagcaccac accgacttga cgccgcaacc aggcagggtc gatctgcgcg agatcaacac
     1801 cgtccaccag aacccggcca cgctctggca catagaggcg ttgcatcagc ttggcaatcg
     1861 tgctttttcc agatcctgag cgccccacca caccgacgac ctgccctgga tgcagagtaa
     1921 gagaaacacg ctgcagcacc aacggcccat cgacacgata ccggaacgcc acatcgtcaa
     1981 atacaatctg cccggcaatc tgcggaagag tcgtgcggtt gggattgtag gacggttcta
     2041 gcttggcatt gaggacatcc cccagtcgtt gtatcgagat gccgacttgt tgaaattcct
     2101 gccagaggtt caccatacgc agtaatgggc cggttacttg tcctgagagc atgttgaagg
     2161 cgatcaactc tccgatgctt aaggcgccgt cgatgacctg ataagcgccc acccacatga
     2221 tggcaatggt cgtagatttc tgaatgcaag tcgctatttg cccagccaca gtaatgagac
     2281 tagtggcccg gaaactggcg gcgacgtatc cggctaattg ctcatcccat cgacgctgga
     2341 gcggtggttc gacggcgagc gccttcacgg tttgaatacc gctcaccgct tccaccagga
     2401 aggattggtt ctccgcgccg cgattgaatt tttcatggag tcgcgcgcga atcgctggcg
     2461 taatgagcac ggagagcaac gcgtataagg gcagcgaggc catcaccacc aatgtgagtg
     2521 tggggctata gatccacatc acgcccaaaa acacgatggt aaacaccaca tccaggacta
     2581 ccgtcaccga gttactggtg ataaattggc ggatctgctc gagttcgcgt acccgcgcca
     2641 ccgtatcgcc gacccgtcgg gcttcaaagt aggctagcgg cagcgcgaga agatggcgaa
     2701 acagttgcgc tcccagcccc acatcaatcc ggttggtcgt atgggcaaac aggtacgtac
     2761 gtagccctcc aagcagcgca tcgaagaggg cgagcgccaa catgccaacc gccataacat
     2821 gaagtgtcgt gaatccttta tggacgagca ctttgtcgat gaccacttgc gtaaagagcg
     2881 gggtcagcaa cgcgaacact tggaggaaga acgaagccag taccaattca cccaacagac
     2941 gtcgatactt gaggacggct ggaataaacc aagtcatgtc gaagctgaca tcttctactc
     3001 gcaagcctga ccgtttggta aaaagcagga ggctaccgct ccagcacgcg acaaactcct
     3061 ccttggaaag aatatgcggc cgctctttca gaggatcctg aaggagggcc ttttctccct
     3121 gtactttggc caggacgacg taggtgccgt cagttcgctg agcaatcgcc ggaagtggcg
     3181 cccccgccag ttcaccccac gaactgctca ccaaaccggc cttgaggccg acgtgcttag
     3241 ccgcacgcac caaggcgttt gtcgtcaaca tatggccaga tagtccaaat tgatgctgaa
     3301 tctggccgct atcgataggc agatcgtgaa accgcgcaat taacagcagg cacaacagtc
     3361 cggtatccgg ccgaggtggc gccgttgaac aagactcgag atgggaagtt ggaacatcca
     3421 tgacgggata gcgagtggcg cgttaggaca gttttgattt ggcgggctgc aggctggcga
     3481 ccatctgctg aagttgttgg tgcaggcgga tcaacgcgtc gagcggcaat gccactctaa
     3541 cggccctgct tcccacaagc tgtttcggca tcgcctctcc cttttgtgca cgctgcgcga
     3601 ctgcaccgat cagactcggc tcgagaaatc caaaatcaat gtaggccaat ccttgtgcga
     3661 gacttgcatg taaatagttc gccagaatcg gctggtcggc ctctgtactc gattgaagac
     3721 gaacgttcac cgccactgct ttatcaccat tctgcttggg actgcttcca acggccatgc
     3781 gcttctcctt taagcaaatg aacgacatac tggaacgaat accccgcnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnncga tatgccggga cctggcatga gatcgcgcgc
     3961 ttgccgatgt ggtttcagcg gcattgcgtg gattcgaaag ccacctacac cgtgcgcaac
     4021 gatggcgcta tcggcgtgcg caatgaatgc gtcactgata ccggtgggca tgatgaagtc
     4081 gaaggggtcg caacagtggt tgatccaggc acgaataccc ggctccgcgt ggtgttcgac
     4141 aatttcttcg cgcggctgtt cggttcatcc cgcgaaggca actattggat tctggcgctg
     4201 gatgctgatt accagaccgc catggtaggg actccggatc gccgatatct gtggattttg
     4261 tcgcgctcgc gccaactcga cgagactgct tacacccgcc tggttgagca ggccagacga
     4321 ttgagatttc ccgtctcaga gcttattcgt ccccaccgat ctgcggcaac atcatagaaa
     4381 cggtagctac gtctcatctc gtctcgtctg atcgctagcc tctcatatca ctgcggccct
     4441 ctgctctcta cggtctctgc cacgaacctc agccctcagt ttcggactcg ccttgcgacg
     4501 caacatgaac cacgacggtc tcctccgttg gtttcacttg cgagccgtgg tgcgtggcgt
     4561 acaccgcaat gaaggccgca ccaaacagaa atacttggga ggcatagtag gcccacagca
     4621 acactaatac taaggaaccg gccgcaccat agaaggccac cagtgtgtta tgcgctaggt
     4681 agacgaccag cagtttgctt ccgagcaagt agagtattgc cgtgatccct gcacccaccc
     4741 acacatccga ccacgccacc tgagcctggg ggacccactt gtagatcatg gcaaacagcg
     4801 tcgagacaac gccgaatgac atcaagagcc caataccctg aaccagtctt cccggcattc
     4861 ccgttgtttc caatacgaac tgaatggcga tgccggtcca ggcgctgagt gtgagggaag
     4921 ccagcaggat gaaggcaatg ccgaacagcc cggacagtga tgcaaatcgg cgtttcaggc
     4981 ggtctcccat ccttggagga tgtttgggtt gaagtcccca gatacaatct aatgcatccc
     5041 tcagctgatc catgactttc agcgcgccga tgacaaagac ccccagcgcg actactccgt
     5101 tcacgagggg ggaccctgcc gatctccact gagcaatgat gcgaaagagt ccattcgctg
     5161 ccgattctcc gatgagttcc ccgatctgcc gctgaaggcc gccaaccatt gactcgtcgc
     5221 ccaccatgct cacggagacc atgatcgtca gcacgaccaa gggcacgatg gtcaacgccg
     5281 tataaaaggc gagcgcggcc gcgaatcgcg gtcctggctc atcgctccat ttcgcaatgg
     5341 tttgtgtcgc gagagacttg agcagggccc acatagactc acgccctcct cttccgccga
     5401 agcctcatat ctccacgttg ggcacgatgc acatcagcct gtggcccatc tatcgatagg
     5461 acacgcagag catggaagcg gtggtggcat ctgactcagg tcagaccggc agcgaagatg
     5521 ggattgccac cgccgttcag cctgtcggtt tgtcatctgg tctgtgtggc gtggtgggtc
     5581 gttggagagc ttcagtggca atggcttcga tcttacaaca ggcatcatag gcatggtcat
     5641 gtcccagcat cgcgccattg cgtgccaagt caatgatgcg ctgaagggcg tcggcatatc
     5701 cctgcatctc ggaattcaag ttggcgcggg cagcagacgc aaggttctgg gccttgtcca
     5761 tgagaagcct ctcactccac agagaagcca ggccaagtgg atgagacggg gacactcaat
     5821 ggctacacac gaataattct cggagccacg acagaccttt gaaagacaca cccaatgtcg
     5881 aacgttgtga ggcggcgccg ttccgaaacg tcactcagag actactcaga gacttccgcc
     5941 ctatctataa aataagaggt ttccccgaaa cacgtggtgg ggacgtagcg gaactcccgg
     6001 taatgtagta caacccgttt tcccatttgc ccatcaagct tcttcgcgac atcatcgtcc
     6061 catacgctaa agttccacag aacgggagcc acgccaggaa ccgtcgtcat cgccaactcg
     6121 ccttcatacg ttttacagac ccaccctttc cgagacagtt tctgtaagta ccctgtgcgg
     6181 tctccgtccg aataattaca gccgcccagg accgctatgc acaacaccag ggagacgtat
     6241 gtcggtacct tcatcatgga tgatgcatta gagcattttc gaggcgggac gatacctagg
     6301 cgtccccctc gtcgaccagt ggaaagactc gccggacaag ctcaggaaca tcccgccgcg
     6361 ccagtggcgc ggagaaaaat agaagagaat aaagttagcc gaacgtcacc gggcggagca
     6421 gttgtgcggt aggatgagtg aggacctatc atgagaagga gacctatgat tgccacactc
     6481 gcgaccgcac tgcttgtcgt tctggccacg gttcccaacg gacatgccgt ggctcaatcc
     6541 agccagcgag atgtctttga tgaacgcgac acccgtgtat ttaaggcagt ggggtgcaca
     6601 caagataagc aaccagccga agcgctgtac tatatcgtcg cgagtcggtc agatctatcg
     6661 gcaggcgttc cgtctccatc agcgcaattc atgaaagacg aggttgaaca gaattggcaa
     6721 cggatcgcct cacagttgac gatggagcaa gtgaccgaag agcgttttac cgatacgtat
     6781 cacgcgctgt tgaatgacat gattccccgt ttacagcggg ccgtggaaga gaagagtggt
     6841 gtatccattg cggtctcgga ggtgaatagc aggccgatgg atcgtacaaa agaccaggac
     6901 gtcccgacct gcagttcaca gtaacggcag tccaccaaac cacccaacca tcgcctcccg
     6961 atcccatcgt ggccgaagag caggcaactc ggtactgctc atctacgcac ttcgtgccga
     7021 gatccacgta ataaaacgcc gctccgctcc actgtggcct ctccagccgc cgattcagac
     7081 cggcttgagc atcgcttgcg gccccagcct ctccgacctg gggttattcc ttgggtcctc
     7141 gttgtcctct tcagtgctaa cgtgaatgag taaccttaca cgaggtgcca tatgagtacg
     7201 atcttattga tcatccttat tctcttgctg ataggcgcgc ttccgtcctg gccgtatagt
     7261 agcggatggg gctattaccc ctccggcggg atcggtctca tcctgctcat cgtgctcatt
     7321 ttggcattaa ccggaagagt ctagacgcgc cccctttgac ggatcctttc gcgaggacgc
     7381 gcggcgttca acgacgaact tgtatcctta aagaagaacg gccgtctgga tgaggcagta
     7441 tcttttatcg tcactgcacg gatggccgat caagttcatc ggataataag gacgggagtt
     7501 ccaccgccag cggatcttcc agttcgatcg gttcgtaatg gacggctaca atggaagtga
     7561 gcggacagga aagccatcca ctcatgccgc gcgttttgaa acgaatgccc atcggcggaa
     7621 gggcctcaag atcgacgctg gcaatggtga taccctcgcg gtcagtccat tgaaatgtat
     7681 ccatcgtcag tgatccaggt atcacctcat accaggaacc atctcggaga aggacgcgag
     7741 cgactgtcgt gacatcaatc ttcaggctca tggctcctcc ctcgtcgtcg cccccttgac
     7801 ggcctctttc ttgccccagg agacgttcgg ggtgtggcgt cacctcgatc gccttgtcct
     7861 ttgttaacca ccgttgtggc ctttcgccgt cgatcctggc tcaatcaact cgcgcctggc
     7921 agtgtggtgt ggcataaggg ccgcccttta ttggcagggt aaagcctaat gacgctgatg
     7981 catgctaggt attcccctat tggatccaga agacacaaag gaaggcaggt agacggtcat
     8041 ctcaaaaccc acaagaacga tcgcaacaaa aaagcgcaca gctcctctag atcgaggaac
     8101 ctgtgcgctt accccaagca gggcttcccg ttatcgctca agaggccgcg tgttcgcgca
     8161 acccgctctg gttctcgatg ttattcgcta cctgcagaaa ccccgttaca cacgaaggtc
     8221 ttcctacttt agacgcttcc ggggagtgcc cggcttatcg acggccttcc ctcagcacgt
     8281 cttccaaatg ctgccgcaac gtaccgagct ctttggattt aaacgagctt ttcgtgtgct
     8341 ccatgacttg gatagcctct tcaattgccg cttcgtaccg gtccaatcga gggcatttca
     8401 tcactgcgca gatatcggtc ggggcctgtg gttgacccgt tcgagacgcc tccagagtgc
     8461 ggccgaccag cgccgataac tggctgatat gccccatcgc ctgagccata tactcctgcg
     8521 ccgtccattc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggatgtcgcc
     8641 tggcgacagt ttcaagaaat tccagttgcg agtttcccgg catccggaca tcagccagga
     8701 gcagatcata tccgttttcg aggagccgct cggcctcaat ggcatcccgg gcggcattgc
     8761 aggcgtaccc tgcgtcggcg agtatggcta cggtcgattc tcgaaacgtc tcctcatcat
     8821 cagcaatgag aatgcgtttt ggcgtaatcg tcatcttcct gctccttttt caacgcggct
     8881 cctgtgccct gctgcccgac accatctgtg acattcagag gatgtatcac cgtaaatgtt
     8941 gatccgcccg ccggccgcgt atggacctcg atcctgccac ccatggctcg gacaagcccg
     9001 cgggacacag agagtcccag tccgagcccc tgcggaccgc cggccgattt cgtcgtaaaa
     9061 aatggttcaa aaatatgcgg aaatacctcg ggggtaatgc ccggcccatg atccatcacg
     9121 ctccaatgga gcgtgtcacc gtgccgggcc accttcaaca ccacttccat atcctgggga
     9181 gacgcatcga tggcgttctg cacgagattg agcaatacct gaaacagatc ggcgcgcggc
     9241 agggtcacca ccggacaggt caactctatt tctgtgcgta actggattcg gcgcgagacc
     9301 agcttgtagt ccagcaacgt tcccacattt tggagcaggt cgctcacgtc cgtggcttgc
     9361 cattgtcccg atgctccatt ctgatagagc gtgtacattt tcttcacaat ggccgcgagg
     9421 cgctggatct ctcgatcgat gagttcaacg aatcgataat gccggtggtc ggggctcacg
     9481 ccttgcttga cgaggtgaaa cgcgttctta atacccgcca gtggattgtt gatttcatgg
     9541 gcgatatcgg ccgccagatg gccaagcgcc gccacttttt cggcctgcga ccgttgagcc
     9601 tccaattcat gaattcttgc ggttcgctca cgcacacgtt gctcaagctg agtttgcact
     9661 tccaccaaag cctgctctgc tcgacgacgt tccgtgatgt catggaatga ggctacgacg
     9721 gcatacggac gttcggcatg aggctcccga agaggctgcg tattaatcga caaccatcgt
     9781 tgttctccgc ccggtcggca tacacccatg atcacgtccg cgcagggctg ccccgtatgg
     9841 agcgtgacca tggcaggatg ctgctccccc tcaaacggcg tgccatcttt gtgaatagcc
     9901 cgccactcag aagtgacgga tgttctcccc atcatctgtt ctgcagtcag gcccagaatc
     9961 tgttccgcac tggggttaca cgcaatgatc cgtccttgcg cattttgcag cacgatgcct
    10021 tccgccaacg ccgtgacgac gccccgatat cgctcagccg tttcaaccaa cgccgttttg
    10081 gtgcgtcgcg cttcagtgac atcttcagcc acataggaat accggtcccg cccactcggc
    10141 ccggtgccga tccaacagac cgagacggac aagtaccgct cctcccccga ggtcggacat
    10201 gtatggggat attcaaatcg caccggttgt gcgctctctc ggctcatctc gtagtgtcga
    10261 tgccacaatg cgatggtgtc cggcggcgcc ccgagcttcg aggcccactt tccctgcatc
    10321 aactccggag tggtgccaaa aaagccggcc gcgcaacgat tgtctgaaag gtgcagaata
    10381 tcgccgtcgt gtaactccac cacccccatc aacatcactc cgctctcgaa caaacctcga
    10441 agcgtggact cacttcgaga cagcgcctct tccgctcgct ttcgctccgt aatgtccaca
    10501 tgcgaacccg tcatgcgccg ggggcgcccc tgctcatctc gttgaaacac ggcgcaggtg
    10561 tggatccatc gatacgttcc gtctttatga cgtaaccgat gctcgagttc atacgacgcg
    10621 atccggcccg aaaggtagtc gtccaccaag gctaacgcac gcttcttgtc atccggatgc
    10681 agccgggatt cccattcaga tggcgaattg gagatttctt gctcctcata tcccagactc
    10741 gacttccaac ggggggagta atacatcgtg tttgcctgca ggtcccagtc ccaaattccg
    10801 gtcttcgtcg cggctacgac taaatccaac cgttcctgag cgtcatgccg cgccacttca
    10861 gcctgcaagc gagctgtcat gtccgtggcc acaccaccga ccgcgatgat tcgccccgct
    10921 tcgtcgcgaa ggggaaactt gcgcaccaca tagaagcgag gatcgtgatt cagcaacgca
    10981 tcttcctcga agctcatcac tccgccggca tgcacgacct cacaatcatg ttgtcggtaa
    11041 ctgtctgcga tatctcgggg aaacaggtca tgatccgtct ttccaatcac ccgctcggcc
    11101 ggaacgccga ccaacgtgag gaattgctcg ctgacttcga gatagcggcc ctgaagatct
    11161 ttgacaaaga tgatggtttg gctatgagtg agaagagagc gcaatcgaat agacacatcg
    11221 tgagcacggc tgcgagcaca tttctgccac tgggagacaa tccacgccgt cagccaggca
    11281 gcaccaattg aaagggctcg attggtgacc tcaatccagc cgatactctc gggagaagtc
    11341 ccaatgagcc cgaccaccgt gagaccggtc gaaatcgccg ctacaatcca ctgcatccgc
    11401 gagcctgaca ccagcccgac cgcggcgacg gcgagcagat acagcaggct ggctggaatg
    11461 cctgctgggg tatagaggtc aatcaggagc gtagcgagaa ggatacacgc actgagggca
    11521 taccaaggcc tgtcaggata gttcaaggcg aacccaccgt tagatggcag aaggccaggg
    11581 gcagctgatt tggtcacaga aagactccgt acgtcacgct ctcgcaacga cgccggatca
    11641 tgaggcgggc aagcgatgca gcttcgcggt cgagatcccc gtggcgcgac tcggccactt
    11701 atcgatctac gagagatcct ctcccacgac agacaccatc ctccatgatg ccgtcaacca
    11761 cttcgtattg ctgtctaggt gcggcctact ttatccgaaa aggggccttc ccggaaaaag
    11821 tctggcaaca aatatcgatg agaaagtgtg gtgtccccaa cggga
//
LOCUS       DAOQDS010000094        11360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_2768_length_11360_cov_14.085449, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000094 DAOQDS010000000
VERSION     DAOQDS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11360
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2768_length_11360_cov_14.085449"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            408..2045
                     /locus_tag="PK589_16800"
     CDS             408..2045
                     /locus_tag="PK589_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21324.1"
                     /translation="MKRSAFALGASLAVLWGSLTLPAQAVEEGQFIKKDGKWEYYSGE
                     DPGLKYLLLKGIITQDEYDRGLRVIEKREQVSKPNYTIDVNNGLNFRVGDKFFLKLRL
                     LTQVRYTHSAYNSAWGTVGDSRNPEILGGQVEYRAIRKQSDSNQFSVPRARLQFLGYA
                     FDPDFRYNISWAFDQTTWNQEGGSGRAGLLDAYVSSWHIPWLTVQAGQQRVWFNRATI
                     SSMATSTFADNLIVQNAFAANQQNARDVGVSISSDEDQYKFNYAFGIWGGVGANLARE
                     GTSVSQNVLPQTGTPGAPGAATTRTYNYDTRFMTGEMMYTARLLYKIAGNPGYGQGDI
                     LNSRTLQSAIAFGYAYNPAQNYLSSIRSDIVDRAYRQAVVKAYNGRLLAGGIWDFQTY
                     EADFIAKYQGWSFQAEGYYRHQRLRNANSGTSAFDLNTIPAAQFLGPAVNLGQAYGWY
                     AQVGKYIIPRKLELAVRYGFMDPSTQQVNDLVKEFGAAINYSFDGTYNNRLVIDYSNI
                     TMGSGGRAPDRFPFESQPGFGRDLVENRINVQYQFYF"
     gene            2085..2909
                     /gene="modA"
                     /locus_tag="PK589_16805"
     CDS             2085..2909
                     /gene="modA"
                     /locus_tag="PK589_16805"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNJ21325.1"
                     /translation="MRAAFRIRGLIAIVGFLISLGLTAQPALAQPETITIAAANSLKD
                     ALRKVLPLFEAEHRNINVRVIYGPSQTLRSQIEQGAPVDIFLPSLFEEIDQLEAKGLI
                     IQGTKRAFAATSLVLITGTALPAPISTIQDLQTIPIRHIAVGDPKTSSVGKVSAQFLK
                     YSKLEPKLKSQYVLGEHSRAVLDLVAKGEAEIGVVYRTDAISNPGVRILDTAPVGSHT
                     PVRYGVAAAWTAQNITAAGDFIEFLLSPTIQTQLQEFGFDRVSPEVGFAQREEVKP"
     gene            2906..3640
                     /locus_tag="PK589_16810"
     CDS             2906..3640
                     /locus_tag="PK589_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21326.1"
                     /translation="MNGIGHPQQHSLSRHTNLILAFIGVMVAAGCAAPTPTPPKTVYQ
                     SGLNQVRIEKDPASTINAHPASLSATEVGTLLRGVRVWERRNALHRLFVGPADKIRAF
                     RDGEIAILAPALAKALSQASPSERVYYHLSHATEHGEEETSTGWLSIQDATLHLALRE
                     AHDRHGPGPDISKYDRQMPNVPERSPAFDATFEPEEYLVKVRSGGSLFAPDQQEELLI
                     RYREALAAMPAQPGLERESKPVPERH"
     gene            3665..4012
                     /locus_tag="PK589_16815"
     CDS             3665..4012
                     /locus_tag="PK589_16815"
                     /inference="COORDINATES: protein motif:HMM:NF021593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HNJ21327.1"
                     /translation="MAILVVGGDSVSAITERAYAGGHGCVQHWSGRKTRDLMRSIPKD
                     TEAVVLVLDRVNHTLARKVRTEATRRGLPVFFQKRGRQVRAAGDRPLDVVHWLDTKDT
                     GLRKHRLDLKTPA"
     gene            4269..5294
                     /locus_tag="PK589_16820"
     CDS             4269..5294
                     /locus_tag="PK589_16820"
                     /inference="COORDINATES: protein motif:HMM:NF008106.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNJ21328.1"
                     /translation="MMTFAHVASALAIVLALTTAALATESPSLLNVSYDPTRELYQEY
                     NAAFIKHWKEKTGNTISIKQSHGGSSKQARAVIDGLGADVVTLALAQDIDAISHQAGL
                     LPENWQTRLPHNSAPYTSTIIFLVRKGNPKGIKDWSDLVKPGLAVITPNPKTSGVARW
                     NYLAAWGYALKQAGGDETKAREFITRLYTNVPVLDSGARGATTTFVERGIGDVLINWE
                     NEILLGGQELGKDKFDIIVPPTSILAEPTVSLVDKVVDKKGTREVAQAYLEYLYSDVG
                     QNIAAKHYYRPQSATVAAQYAGQFPKLTLFTINEVSGSWSVTNKVHFVDGGLFDQVYQ
                     EISRTRK"
     gene            5371..6207
                     /gene="cysT"
                     /locus_tag="PK589_16825"
     CDS             5371..6207
                     /gene="cysT"
                     /locus_tag="PK589_16825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNJ21329.1"
                     /translation="MSILLKQPTVLPGFGLTLGFTLFYLSLIVLIPLAGLFVKTSALT
                     WDQFWQAVSDPRTVASYQLTFGLSFVAALINAFFGVIIAWVLVRYAFPGRKFVDALVD
                     LPFALPTAVAGIALTAVFSVNGWLGQYLEPLGIKVAFTPLGVLVALTFIGLPFVVRTV
                     QPVLQDLEKEIEEAAASLGATRWKTISKVIFPEVFPALLTGFTSAFARALGEYGSVVF
                     ISGNMPMRTEITPLLIITKLEQYDYAGAAAIAVVMLVASFVLLLIINGLQWWSGRRQV
                     AL"
     gene            6225..7103
                     /gene="cysW"
                     /locus_tag="PK589_16830"
     CDS             6225..7103
                     /gene="cysW"
                     /locus_tag="PK589_16830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNJ21330.1"
                     /translation="MTTGILGLDIRTPRIASTVAEPFLVRLALILVSLTFLALFLFVP
                     LAAILFEALKHGLGAYLASFQEPDAIAAIELTLLATAIALPLNVVFGIAAAWAIAKFE
                     FWGKQVLITLIDLPFSVSPVVAGLIYVLLFGLQGSLGPWLAAHDIKIIFAIPGIVLAT
                     IFVTFPFVARELVPHMQAQGKDEEEAALVLGATGWQTFWRVTLPSIKWSLLYGVILCN
                     ARAMGEFGAVSVVSGHIRGETNTMPLHVEILYNEYNYVAAFAVASLLALLSLVTLAAK
                     SLVEWTSSVEQKENRP"
     gene            7100..8167
                     /locus_tag="PK589_16835"
     CDS             7100..8167
                     /locus_tag="PK589_16835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HNJ21331.1"
                     /translation="MSIEVRNVTKTFQTFTALHEINLHVKTGELIALLGPSGCGKTTL
                     LRILAGLEGPTTGSILLNDQDVTDRHIGERGVGFVFQHYALFRHMTVFENIAFGLRVR
                     PRATRPPDAAIDAKVRELLKLVQLEWLGDRYPRQLSGGQRQRVALARALAVEPTVLLL
                     DEPFGALDAKVRKELRRWLRRLHNDLHVTSVFVTHDQDEALEVADRVVVMNKGRIEQV
                     GTPEEVYDQPANPFVYDFLGGVNLFHGRVHEGRVQVGELKLALREESEVPDGPAIGYV
                     RPHEIDLHRQPGEEGAIEALVGRVQPVGAVARLEMVRLDNGDVIEGEVSQEQWRSLAL
                     SKGERVFVRPKNLRVFPEVAE"
     gene            8172..9305
                     /locus_tag="PK589_16840"
     CDS             8172..9305
                     /locus_tag="PK589_16840"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ21332.1"
                     /translation="MRDPRLDPEADYDLPTADKPDSRVARPSKVLSASDPQAMVQSIR
                     ASCLVFEDSASQALLQRVRRIAPTETTALIIGETGTGKKLIARHVHELSTRRTGPFIA
                     VNCAAITESLMESELFGHERGAFTGASSTKAGWFETAQGGSLFLDEIGDLPLALQAKL
                     LRVIQQREVVRVGARRPLPVDVRLIAATNVKLEEAVAAGRFRADLYYRLHVVTLQLPP
                     LRERRDDILPLATHFLSLYGSRLGFTQARLSPEATDQLLQYPWPGNIRELENTIHHAL
                     VVCPGTEIRVSDLRLPSGQYDVHVTKPAAETALTGLEQALQSLFQADHPALHRRIEET
                     VFRTAYDFCDRNQVRTAQLLGISRNIVRDRLIRYGLLPSLARQ"
     gene            complement(9337..9507)
                     /locus_tag="PK589_16845"
     CDS             complement(9337..9507)
                     /locus_tag="PK589_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21333.1"
                     /translation="MGVLIIGALSLTVLVWGLITSLANEADAEKRRLAQRRLREDGSD
                     DRAGASESLLAA"
     gene            complement(9613..10074)
                     /locus_tag="PK589_16850"
     CDS             complement(9613..10074)
                     /locus_tag="PK589_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21334.1"
                     /translation="MSRLGDKARTYRLPTIINGALTYKTLELDRGRWVAVCFVPKLGV
                     IEADLLDRYATDVELLGATLVLVSMDAGRWPRDSFASLPHVRIPVVGDPLGRLQRLFG
                     GATARSWGRARTFLLDPDGHFRFHLMHSLSERGMGVFMELLRTYQDEEVPA"
     gene            complement(10129..10443)
                     /locus_tag="PK589_16855"
     CDS             complement(10129..10443)
                     /locus_tag="PK589_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21335.1"
                     /translation="MSSHTRTDSAHQADSRLVDAWLLENLRWNGPQTLDYLSRLFPKL
                     EEARLLFAIDRLSRNGQVVISPPQNGDYLISVRSIGQEGLREDQHGSCRRAHERAPSA
                     VQ"
     gene            complement(10692..11057)
                     /locus_tag="PK589_16860"
     CDS             complement(10692..11057)
                     /locus_tag="PK589_16860"
                     /inference="COORDINATES: protein motif:HMM:NF021460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2173 family protein"
                     /protein_id="HNJ21336.1"
                     /translation="MAKSLDELLTLKGVIASGEFAENGRLIAFRSHGAPLPDDVALLT
                     AQFSAAISQLLGVLAAAHSRLSGFNWIPQQGWAYSGGDLTIAVGGSRGVFVRTAEADF
                     NQLFEALIGSREPVGAEVR"
BASE COUNT         2468 a         3460 c         3199 g         2233 t
ORIGIN      
        1 tggccatacg taatgcccgc agtgaattca cgacattgga cgatggttca gctttgcttt
       61 tttcactcat gatggcggac atgtatcatg atagataaat ttgtgtcaac tatcattgca
      121 gagtttcgac cagcagcaac gcaaacgtcc agcgggatct agagaacgtt tcgctgactg
      181 tgaaggctct gcattgggat caatgcacct cagaaaagtc gtgtttgacg gagccgaaag
      241 agaaagaggc attgaatggt tttattgttg acaaacttat taaagataat atatttcata
      301 cctcatcgga aattagttat ttaataacat gttgaaccta tggtgatcgt aaccattaaa
      361 tcaccattta ctcagtcatt ctaaacattc acatgggagc ggacgtgatg aaacgatcag
      421 catttgccct tggggcaagc ctagccgtcc tctgggggag cctgacgtta ccggctcagg
      481 cagtcgagga aggccagttc atcaagaagg acggaaagtg ggagtactat tcaggagaag
      541 accccggtct gaaatatctc ctgctcaagg gcatcatcac ccaggacgaa tacgaccgcg
      601 gccttcgagt catcgaaaaa cgcgaacagg tttcaaaacc gaactacaca atcgatgtga
      661 acaatggttt gaacttccgc gtgggggaca aattcttcct gaagctccgg ctgctcactc
      721 aggtccgcta tacccacagc gcctacaaca gtgcctgggg aacggttggt gattcgcgca
      781 atcctgaaat tctcggcggc caggtcgaat accgcgcgat ccggaaacag agcgattcca
      841 accaattcag cgtaccccgt gccagactgc aattcttggg ttatgccttc gacccggatt
      901 ttcgctacaa catttcctgg gcgttcgatc aaacaacctg gaaccaggaa ggcgggagcg
      961 gcagagccgg cctactcgat gcctatgtct cgtcgtggca cattccctgg cttacggtgc
     1021 aagccggtca acagagggtc tggttcaatc gcgccaccat cagctcgatg gccacatcca
     1081 ccttcgccga caacctgatc gtccaaaacg ccttcgcggc caaccagcaa aacgcgcgcg
     1141 acgtcggtgt cagcatttcc agcgacgagg atcaatacaa gttcaattat gcgttcggta
     1201 tctggggcgg agtgggggcg aatctcgcaa gggaaggcac ctcggtcagc caaaatgtct
     1261 tgccccagac cggcacgccg ggggcccccg gcgcggcgac tacacgaacc tataactacg
     1321 acacgcgctt catgaccggc gagatgatgt atacggcccg gctattgtac aaaatcgcgg
     1381 gaaaccccgg ctacggccag ggtgacatct tgaactcgcg gacgctgcag tcggcaatcg
     1441 cattcggcta tgcgtataac ccggcgcaaa actatctgag ctcgattcgc tccgatattg
     1501 tcgatcgggc ctaccgccag gccgtcgtaa aggcctacaa cggtcgactg ctagccggcg
     1561 gcatctggga ttttcagacc tatgaagcgg acttcatcgc gaagtatcag ggctggtcat
     1621 tccaggcgga aggctactac cggcaccaac gcttgcgaaa cgccaatagc ggtaccagcg
     1681 cgttcgacct gaacacgatc ccggcggcgc aatttctcgg cccggcagtg aacctcggcc
     1741 aggcgtatgg ctggtatgcc caagtgggaa aatacatcat cccgcgcaaa ctcgaactcg
     1801 cggtgcggta tgggttcatg gatccatcca cccaacaggt gaacgatctg gtcaaagaat
     1861 tcggcgcggc gatcaactat agtttcgacg gcacgtacaa caaccgcctc gtgatcgatt
     1921 attcgaacat cacgatgggc agcgggggac gagcgccgga ccggtttcct tttgaaagcc
     1981 aaccgggatt cggacgtgat ctcgtcgaga accggattaa tgtgcaatac cagttctact
     2041 tctagaaatc tatgacacgc cattacaggt ttttggagga catcatgaga gcagcatttc
     2101 gtatcagagg actcattgcc atagtcggat tcttgatcag cctgggactg accgcccagc
     2161 cggccctggc gcagccggaa accatcacca tcgccgccgc caacagcctc aaggacgcgc
     2221 ttcgaaaggt gcttccgctg tttgaggcag agcacaggaa catcaatgtg cgggtgattt
     2281 acgggccgtc gcaaacactc cgcagccaga tcgaacaggg cgcgccggtc gatatctttc
     2341 tgccgtcgct cttcgaagaa atcgatcagc tcgaagcgaa agggctgatc attcaaggga
     2401 ccaaacgggc ctttgcagca acgtccctgg tcttgattac gggaaccgcc ctccccgccc
     2461 ccatcagcac gattcaggat ttgcaaacga tcccgatacg acacattgcc gtcggggacc
     2521 cgaagacgtc atcggtggga aaagtttcag cgcagttctt gaagtacagc aaactcgagc
     2581 caaagctgaa gtcacaatac gtcctcggtg aacactctcg agccgtgctc gacctggtgg
     2641 ccaagggcga ggctgaaatc ggcgtggtct atcgcaccga tgccatctcc aacccgggcg
     2701 tgcgcattct ggataccgct ccggtcggct ctcacacccc ggtacgctac ggcgtcgccg
     2761 ctgcctggac ggcccagaac atcaccgcgg ccggcgattt cattgaattt ttactcagcc
     2821 ccacgatcca gactcaattg caggaattcg gattcgaccg cgtatccccc gaagtcggat
     2881 tcgctcaacg agaagaggtg aaaccatgaa cggcatcgga catccacagc agcattcgct
     2941 atcccgccac accaatctga tcctcgcctt catcggcgtg atggtggcgg ccggttgcgc
     3001 agcaccaact ccgacgccgc ccaaaacggt ttaccagtcg ggattgaatc aagtgcgaat
     3061 cgagaaggat ccggcgtcaa ccatcaacgc ccatccggcc tcattgagcg ccacggaagt
     3121 cgggaccttg ttacgaggcg tgcgagtctg ggaacgacgc aacgcactgc accggctctt
     3181 tgtcggaccg gccgacaaaa tcagagcctt ccgcgacgga gaaattgcga tcctggcgcc
     3241 ggcgttggca aaagcgctgt cgcaagcctc gccttctgaa cgcgtgtact accatctcag
     3301 ccatgcgacc gagcatggcg aagaggagac cagcacggga tggctctcga tacaagacgc
     3361 gacgctgcat ctggcgttgc gcgaagccca cgaccggcat gggccgggac ccgatatcag
     3421 taagtatgac cggcagatgc cgaacgtgcc ggaacggtct ccggccttcg acgccacctt
     3481 tgagccggaa gaatacctgg tgaaggtccg gtcaggtgga agtctcttcg caccggatca
     3541 gcaggaagaa ttgctcatcc gataccggga ggcgttggct gccatgcccg cgcaaccggg
     3601 attggaacga gagagcaaac cggtgccgga acggcactga gcgatcgaga aggaaggagc
     3661 atccatggca atcctggtcg tcggcggtga ttcggtcagc gccatcacag aacgggccta
     3721 cgccggcggc cacggatgcg tccaacattg gagcggacgc aagaccaggg atctcatgag
     3781 gtcgattccc aaagatacgg aggccgtggt gctggtgctg gatcgagtga accatacatt
     3841 agccaggaaa gttcgaaccg aagccacacg ccgcggccta cccgtctttt ttcagaagcg
     3901 agggcgccaa gttcgggcgg ccggtgaccg gccgctcgac gtggtgcact ggctcgacac
     3961 aaaagacacc ggtctacgaa aacatcgcct ggacctaaag acgccagcat agtccgtcca
     4021 cttcgtatca tggcgattga accttccacg tgaaccgaca gaaaggggaa gagagggcaa
     4081 accacatgac cacattccaa acgccgacca tctcagccct gagggttttc tacgcacgca
     4141 tgtgcgtacc gcatccctgt tgaccttcga ccgtccctac tgattcgaac agcgtgccag
     4201 tgaagaaccg tgaagcaggt cgccgatctg ccgaatcgat atccgtgcgt ctttatgaag
     4261 gagagatcat gatgactttc gcacatgtcg ctagcgccct cgcgattgta ctcgcgctga
     4321 ccaccgctgc gctggctact gagtcgccct cgctattaaa cgtgtcctac gaccctaccc
     4381 gcgagctgta ccaggaatat aacgccgcct ttatcaaaca ttggaaggaa aagacgggca
     4441 acacgatcag tatcaagcag tcgcatggag gatcgagcaa gcaggcacgg gcggtcatcg
     4501 acggcctcgg cgccgacgta gtaacgctcg cgctcgcgca ggatatcgac gccatttccc
     4561 accaggccgg gctgcttccg gaaaactggc agactcgcct cccccacaac agcgcaccct
     4621 acacctcgac gatcatcttc ctggtgcgca aaggcaaccc gaaaggcatc aaggattgga
     4681 gcgacctggt gaagccgggc ctggcagtca tcacgcctaa ccccaaaacc tccggcgtcg
     4741 cccgctggaa ctacttagca gcctggggtt atgcccttaa gcaggcgggt ggcgacgaaa
     4801 ccaaagcgcg ggaattcatc acccgactct atacgaacgt gcccgtcctc gactcaggcg
     4861 cgcggggtgc cacgaccacc ttcgtcgagc gtggcatcgg cgatgtgttg atcaactggg
     4921 aaaatgaaat cctcctgggc ggccaagaat taggcaaaga caaattcgac atcatcgtgc
     4981 ccccgaccag catcctcgcg gaacccacgg tcagcctggt ggacaaggtc gtcgataaaa
     5041 aaggtacgag agaagtggcg caagcctacc tggaatatct ctactccgac gtggggcaga
     5101 acattgccgc caaacattac tatcggcctc aatcagccac ggtggcggcc caatatgccg
     5161 gtcagtttcc gaagctgacc ctgttcacca tcaatgaagt ctccggaagc tggagtgtga
     5221 ccaacaaggt ccacttcgta gacggcggcc tgttcgatca ggtctatcaa gagatttcac
     5281 gcaccagaaa gtgaccaccc ttgtgccggt ccggccgatg atccacgttc accggccgga
     5341 tcggcacgca tggccactca aggagccatc atgtcgatct tgttgaaaca gccgacggta
     5401 ttaccgggct tcggcctcac actcgggttt accctgttct atctcagcct gatcgtgttg
     5461 attccgttgg ccggtctgtt cgtgaagacg tctgcgctga cctgggacca attctggcag
     5521 gccgtctccg acccgcggac ggtggcctcg taccaattga cgttcgggct atccttcgtg
     5581 gccgccctga tcaatgcctt cttcggcgtc atcatcgcct gggtcctcgt gcgctatgcc
     5641 ttccccggca ggaagttcgt ggatgcgctg gtcgatctcc ccttcgcgct cccgaccgcc
     5701 gtggccggca tcgccttgac cgccgtgttc tcggtgaacg gatggctcgg ccaatatctg
     5761 gagccgttgg gaatcaaggt cgccttcacg ccgttgggtg tactggtcgc tctgaccttt
     5821 atcggcctgc ccttcgtggt gcggaccgtt cagccggtcc tgcaggatct ggaaaaggaa
     5881 atcgaagagg cggccgccag cctcggcgcg acccgctgga aaaccatctc caaggtgatc
     5941 tttcccgagg tgttccccgc gctgttgacc gggttcacct cggccttcgc cagggcgctg
     6001 ggagaatacg gctccgtcgt cttcatctcc ggcaacatgc cgatgcggac agaaatcacg
     6061 ccgctgctca tcatcacgaa actggaacag tacgactatg ccggggcggc agccatcgcg
     6121 gtcgtcatgc tggtggcttc atttgttctg ctcctgatca tcaacgggct gcagtggtgg
     6181 agcggcaggc ggcaagtcgc gttgtaagcg acctgggagg gattatgaca accggtatct
     6241 tgggtctgga catacgcacg ccccgcattg cctcgaccgt cgccgagccg ttcctcgtgc
     6301 gcctggcgct gatcctggtt tcgttgacgt ttctggcgct gttcctattc gtgccgctgg
     6361 cggccattct cttcgaagcg ctcaagcacg gcctcggcgc ctatctggcc tcgtttcagg
     6421 aaccggatgc catcgccgcc attgaactca cgcttctcgc caccgccatt gccctcccgc
     6481 tgaatgtcgt cttcggcata gcggccgcct gggcgatcgc caaattcgag ttctggggta
     6541 agcaggtcct gatcaccttg atcgaccttc ccttctccgt ctcgccggtt gtcgcgggat
     6601 tgatctacgt actgctcttt ggtttgcagg ggtccctcgg gccctggctg gccgcgcatg
     6661 acatcaagat catcttcgct atccccggca tcgtgctggc caccatcttc gtcacctttc
     6721 ccttcgtcgc gcgcgaactg gttccccaca tgcaggcgca gggcaaggac gaagaagaag
     6781 cggcgctggt gctgggcgcc accggctggc agaccttctg gcgcgtgacc ctgccctcca
     6841 tcaagtggag cctgctgtac ggggtgattc tctgcaacgc gcgcgccatg ggagagttcg
     6901 gcgcggtgtc cgtcgtgtcc ggccatattc gcggcgaaac caacaccatg ccgctgcatg
     6961 tcgagattct ctacaacgaa tacaactacg tcgccgcatt tgccgtcgcc tccctgctgg
     7021 ccttgttgtc gctggtcacc ctggctgcca agagcctggt ggaatggacc tcgtcggtcg
     7081 aacagaagga gaaccgccca tgagcatcga agtccgcaac gtcacgaaga cctttcaaac
     7141 gtttactgcg ctgcacgaga tcaatctgca cgtgaagacc ggagagctga tcgccctgct
     7201 ggggccgtcg ggatgcggaa aaaccacgct gctgcgcatt ctcgccgggc tggagggacc
     7261 gaccaccgga tcgattctgt tgaacgatca ggacgtcacc gatcgccata tcggcgagcg
     7321 cggcgtcggg ttcgtctttc agcactatgc cctgttccgc catatgaccg tcttcgagaa
     7381 catcgcgttc gggctcagag tccggcccag agccacccga ccccctgatg cagccatcga
     7441 cgcgaaggtg cgcgagttac tcaagctggt gcagttggag tggctgggcg atcgataccc
     7501 gcggcaactc tcaggcggcc aacgtcaacg cgtcgcgctc gctcgggccc tggccgtcga
     7561 gccgaccgtc ctgttgctcg acgaaccctt cggcgcactc gatgccaagg tgcgcaagga
     7621 acttcggcgc tggctgcgcc ggctgcacaa cgacctccat gtgacgagtg tgttcgtgac
     7681 ccacgaccag gatgaagcgc tcgaagtcgc tgatcgcgtg gtcgtgatga acaaaggccg
     7741 catcgagcag gtgggaactc cggaagaggt ctatgatcaa ccagccaatc cgttcgtcta
     7801 cgactttctg ggcggcgtga atctcttcca tggccgggtc catgaaggcc gcgtccaggt
     7861 cggcgaattg aagctggcac tccgcgagga atcggaggtc cccgacggtc cggcgatcgg
     7921 ctacgtccgt ccgcatgaaa tcgacctgca ccgccaacca ggagaagagg gtgcgatcga
     7981 ggccttggtt gggcgggtac agccggttgg agccgtcgcg cgcctcgaaa tggttcgact
     8041 ggacaacggg gatgtcatcg aaggagaagt cagtcaggaa caatggcgct cactggcctt
     8101 gagcaaggga gagcgggtct tcgtcagacc gaaaaacctg cgtgtcttcc cggaggtcgc
     8161 ggagtaagcc catgcgagat ccccgtctcg acccggaagc ggattacgac ttgccaacgg
     8221 ccgataagcc ggactcccgc gtcgcacggc cgagcaaggt cctcagcgcc tcggacccgc
     8281 aggcgatggt ccaatcgatc agggccagct gtttggtctt cgaagatagc gcctcccagg
     8341 ccctcttgca gcgcgtgcgt cgcatcgcgc caaccgaaac tacggcgctt atcatcggcg
     8401 aaaccggcac cggcaagaaa ctgatcgccc ggcatgtgca tgagttgagc acgcgccgca
     8461 cgggcccctt cattgccgta aactgcgccg ccatcacgga gtcgctgatg gagagcgagt
     8521 tgttcggaca cgaacgcggg gccttcacgg gcgcctcctc caccaaagcc ggctggttcg
     8581 agacggcaca aggcgggtcg ctgtttctgg atgaaatcgg cgacttgccc ttagcccttc
     8641 aggcgaaatt gctacgggtg attcaacagc gggaagtcgt gcgcgtcgga gcacgccggc
     8701 cactcccggt ggatgtccga ttgatcgccg ccacaaacgt gaagctggag gaggccgtcg
     8761 ccgcaggacg attccgcgca gacttgtatt accgcctgca cgtcgtcaca ttgcagcttc
     8821 cgcccttgcg tgagcggcgc gatgacattc ttcccctcgc cacgcatttc ctgtcgttgt
     8881 atggatcgcg cctcggattt acccaggcca ggctctcgcc ggaagcgact gatcagctgc
     8941 tccaataccc ttggccgggc aacatccgcg aattggaaaa cacgattcac catgcattag
     9001 tcgtgtgccc ggggacggag attcgtgtca gcgatctgcg cctgccatcc ggacagtatg
     9061 acgtgcatgt caccaagcct gccgcagaaa cagctctcac cggtttagaa caggcgctcc
     9121 aatcactctt tcaagcggac catccggccc tgcaccgacg gattgaagag acggtgttcc
     9181 gcacggccta cgacttttgc gatcggaatc aggtccgcac tgcccagctc ttgggtatca
     9241 gccgcaacat cgtccgtgat cgtctgatcc gctacggatt gctcccgtca ttggcccggc
     9301 agtagatgga tcaggacagg aggccagcac tttgccctat gcggccagca gcgattcaga
     9361 agctccggca cgatcgtcac tgccatcttc gcggagccgc cgttgcgcca gacgacgctt
     9421 ctcggcatcc gcctcattcg cgagactcgt tatcaggccc cagaccaata ccgtcaggga
     9481 caaggctcca ataatcagca cgcccatacg ccacctcttg ccttccattg atgaacgaac
     9541 ctcttctcat tgcggaactt cagcaacatc gacaggaatg gcagcaacac aggcgatgaa
     9601 agactcctcc gttcacgctg gaacctcctc gtcctgatac gttcggagca attccataaa
     9661 gacgcccatg cctctctcac tgaggctatg catcaggtga aatcggaaat gaccgtcagg
     9721 atcgaggaga aacgttcgcg ctcgtcccca tgatctggca gtggcaccgc cgaacagtcg
     9781 ttggagacgg cccagtggat ctcccacgac agggatacgg acatgaggca ggctcgcaaa
     9841 cgagtcacga ggccaccggc ccgcatccat cgagacgagg acgagagtag cacccaataa
     9901 ttcgacatcg gttgcgtacc gatccaacag atctgcctca atcacaccga gctttggcac
     9961 gaaacacacc gccacccatc gcccgcgatc aagttcgagc gtcttgtacg tcaacgctcc
    10021 attgatgatt gtcggaagtc gataggtgcg tgctttatcg cctagacgtg acatatcttc
    10081 ctccttgctc caaccgccct tcagcccggg gcagaccatg tcggagcgtt attgtactgc
    10141 cgagggggca cgctcatggg cacgtcggca cgacccgtgt tggtcctcgc gcaatccttc
    10201 ctgcccgatg cttcggacag aaattagata gtcaccattc tgcggcggag aaatcaccac
    10261 ctggccgttc cggctcaaac gatcgatcgc aaagagcagg cgcgcctctt ccagtttcgg
    10321 aaacagccga ctcagatagt cgagcgtctg ggggccattc catctcaggt tctcaagcaa
    10381 ccaggcatcc accagccggc tatcggcttg atgagcgctg tcggtacgcg tatgtgagga
    10441 catgtccacc tccatgctga gcaaaaatat cgtacgtcac gccccggcac gaccacatag
    10501 ggttgtgcgc cgcgagagaa cggccgctca gagttctgca ctcacgtcac aggcctatca
    10561 cgcgcaatcg agccaacaac ccgggaatcg acaagggaaa caggtcgcat cggggaactg
    10621 ttggtgagaa gcgttcatcc ggaggtcctt ggcaaatgaa cgattgcctt catctcgaat
    10681 ggcgcgtcgc actagcgcac ctctgcaccg accggctctc gtgatccgat caaggcctcg
    10741 aacaactggt taaaatccgc ttcggcagtc cgaacaaaca caccccgact tccgcccacg
    10801 gcaatcgtga ggtcgcctcc tgaataggcc cagccttgct gtgggatcca attgaacccg
    10861 ctcagcctgg aatgggccgc ggctaagacc ccgagtaact gactgatggc cgcggaaaac
    10921 tgcgccgtta atagcgccac atcatcaggc aagggggcgc catgcgagcg aaaggcaatg
    10981 agtcttccgt tctccgcaaa ctcccccgag gcaatcacgc ccttgagcgt gagcagttca
    11041 tctaatgact ttgccatgtg attcatcctt tctttagcga gcagcaacgg tggggactct
    11101 gtgtgcgtgg atattgcaga aggcgtgttg ttcaagagac ggaggaattg aagctgtgaa
    11161 cgcctctgct gttggctggg caacaggcgt tgctgggacc gtgttgctgg agcagcagtt
    11221 gcaacggtgc gtgacgattt cgatctccct ccggcagaag aaccttccga gacatgctca
    11281 tgccgcagag tggcaccggg ataagcactg tggtaccgta ggcgctcaca cgaggcctgc
    11341 atgcgctggc tccatgaccg
//
LOCUS       DAOQDS010000095        10878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_2978_length_10878_cov_20.109304, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000095 DAOQDS010000000
VERSION     DAOQDS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2978_length_10878_cov_20.109304"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="PK589_16865"
     CDS             <1..1448
                     /locus_tag="PK589_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ21337.1"
                     /translation="KLPEGASLDRTDAVLRQVVAISKTVDGIAHVVAMTGLNPLQFTN
                     TPNYGIAFLILKPFDERHRSAKEISEEMNQKISAIKEGITFVLMPPPILGLGNGAGYG
                     LFVEDRAGLGFGALQNAVGGLQGAVMQTPGMGYPISGYQANVPQLDAEVDRVKTKTQG
                     IALTDLFATMQIYLGSAYVNDFNLFGRTWRVYAQADGDFRGKVEDISNLKTRNARGEM
                     VPIGSMVKFSQTYGPDPVLRYNGYPAADFMGEADPTKFSSAQAMDTIRDIAGKVLPNG
                     MTIEWTDLSFQEASQGKAALLVFPIAILLAFLVLAALYESWTLPLAVILIVPMCMLCA
                     LAGVKLTGGDNNTFVQVGLVVLMGLACKNAILIVEFARELELQGKGIVEAALEACRLR
                     LRPIVMTSVAFIAGTVPLVLSHGAGAEVRSATGITVFAGMIGVTLFGLFLTPVFYVGL
                     RMLSGRKLIRHDEGMDVARPAGQLSGVENPH"
     gene            complement(1455..2255)
                     /gene="modA"
                     /locus_tag="PK589_16870"
     CDS             complement(1455..2255)
                     /gene="modA"
                     /locus_tag="PK589_16870"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNJ21338.1"
                     /translation="MAVGLVGGLAIGEPAEAENLTIGAAYSLKAAFQEVVPMFESEFG
                     ATVNVVYGSSQTLRREVESGAPVDVLLSAVEDLEKLQKKGLTLNGGPEVYAQTSLVFV
                     MSAASQAMPISFHDLLSERRTRVAIGAFESAVAGPITARALRAVDPSYKDRFRLISAT
                     HHDEVIEMIRREEAEMGLVYRVDAINNSGQVRIIDEMPGGTYTPVRFGQAIVWTCRDE
                     SRTAAEQFTDFFMSPRIQKLLLKYGFEPAASHVGENKVARTAPKVRVN"
     gene            2755..4254
                     /locus_tag="PK589_16875"
     CDS             2755..4254
                     /locus_tag="PK589_16875"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ21339.1"
                     /translation="MNEIVLIALRRPYTFVVMSILIVLLGSITVLHMPTDVFPNITIP
                     VTSVVWAYAGLLPQQVEGRITYLFERFLTSTVEGIKYIHSHSYYGSSITNIFLQDGVD
                     VGRAEADITAIAQTVVKALPPDISPPMVMRLAPSSIPVAMLQISSDRLTPAELYNLAY
                     MRIRPLLVTVPGIVLPHPYGGQDMQVMVNLDQQKLLARNLTPADIHNALMQQYLVLPS
                     GDIKIKQTDWIVQTNASPMQIEHFSDIPVKREDNAFIYLRDVATVRLMGRVQQNAVLV
                     KGKQTVIIVAMKSTEASTLDVVNGIKKMIPRAEQISPEGVEIKLLDDASTFVKDSIAD
                     VLHEMATAGALVGLIVLLLLGSWRATVIVWTSIPLSILTALIGLHWLDETINVMTLGG
                     LALAVGILVDDATVMIENIDRHLEMGKPLEQAIVDAANQIVVPTFVATLCIAIVWIPL
                     FELGGVAGYLFKPMAEAIIIAMLASFILSRTLVPTMAKYMMKGHHVEAA"
     gene            4238..5935
                     /locus_tag="PK589_16880"
     CDS             4238..5935
                     /locus_tag="PK589_16880"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ21340.1"
                     /translation="MSKPLEPAEQPGPVVSGNIFVRFQQGFERGFNRFRERYGLLLER
                     AMARRRAFVTVCLVIAVASLSLFFFLGRDYFPEIRSGVIQMHMRAPLGTRIEVSGRIA
                     TLVSNSVEELLAGEVENIVSNCGLPVGAHNLAFIPTPTIGSQDCDLTILLRDEKSPVW
                     EYRRILRKGLTERYPGTEFTFQPSDLTAKILNFGAPAPIDVQIKGPDMHPNYEFTRKL
                     AGRLRQIPGATDVVIQQTMRTPTLMVEGNRTFGLAVNRTQKEMASNLLMTTAGSQQID
                     QVYWLDPKTGMSYLINVYTPQAQVNSVNSLRTIPVEPSNDVSDNNHDVQLLGNLADLS
                     AEGTPGVITHANIMPLFDIYVGTEGRDLGGVLADVETVAKSMEHELPRSAAIEIHGQA
                     SLMHDAYFELIFGLLAAIVLVYLLIVVNFQSWLDPFIIITALPGALAGIAWALFLTHT
                     RLSEPALTGAIMTMGTATANSILVVSYARERLETHGEALRAAIEAGTTRFRPVLMTAS
                     AMIFGMVPMATGYSQNAPLGRAVIGGLLVATIFTLFFVPCVYAILYDGRSVPAKEQL"
     gene            5935..7137
                     /locus_tag="PK589_16885"
     CDS             5935..7137
                     /locus_tag="PK589_16885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ21341.1"
                     /translation="MLKRLSPRPGVLLGIVLLLFYLSYRIYESNVDATTLRKHTLEDT
                     IPTVAITYPKPVPPTETITLPGNIQAWFEAPIYAQVSGYVKMWYKDYGALVQKGDILA
                     EINAPALDAQYAQAKADLAAEQAKYALADLTAKRWVALRKNHAVSEQSISVQEANAKA
                     EAARVKAAEQNVKNFEALIQFKTIVAPYDGVVTVRNINVGDYINKEGNISNRSSITNL
                     FTVADVSMLRLFVNVPAAFGAFLKPGLTADVTVPQLPDRHFTAKFLTVARGFDVSTRT
                     AVTVFTIDNEDRALWPGSYAKVHLTAPVDRNVLTIPSTALVFQEHGTQVAVVAEDDRV
                     QLKPITVSKLMDNAVEVSGGISSQDRIVNNPSAALLEGDKVRVVTPAPGYDLVGTATP
                     LAKEPALK"
     gene            7134..8768
                     /locus_tag="PK589_16890"
     CDS             7134..8768
                     /locus_tag="PK589_16890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNJ21342.1"
                     /translation="MTCFRSRPAPCVSGERADARRFTDLARTAALLLALSLSACSTFP
                     AVDLSPPYEPAQFVVPASWHGSSPFVEAKPSDGELRQDWWTLFKDPVLNGLEAQALTA
                     NPDLQAAAERFVQSRDVMMKARSQYLPRVGLGFDATDNRQSLHRLFRAPDSPLEESSV
                     VGGGLASWEPDFWSALRNATKVEIYRAEERAADFGLARLSLQAEVAANYFALRGLDAQ
                     DAIYTQSIALYTQSLELVNAQFIGQIASALDVARVESLLFSTETRRAQIQGQRQVLEQ
                     AIAILLNVAPASFTIAPVNDLKMATFTIPQTLPSTLLERRPDIAAMERRMAQANRTIG
                     IARAAFYPNVSFRLGGGFEDEAFNLFSLANSFWSYGSGVSLPLFQGGYRRAQLQQSWS
                     AYRETEDRYRSTVLNAFREVENNLSLTNRLTLAANRQDAAVGATLKTQDLTMELYQGG
                     LASSLELIYAQVAMLTARIESVQIKAELLRTSVALIRSLGGGWNRQQLPTDDEIQPFG
                     TFQYVNLDKPPSVRGIDVNAENNRLHNDLTKKPAVR"
     gene            8877..10364
                     /locus_tag="PK589_16895"
     CDS             8877..10364
                     /locus_tag="PK589_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21343.1"
                     /translation="MMIRNVFGLLLPLALTAQLAGYAALVVHAANEKQGAQHYELSRG
                     AGEKRSECVGMVRIGPGIAAMDLEATRTDSDDAQISLRGTRDSFVCEAILHPAMRAVL
                     GRSIVQFHNGTTLPLAQVLRQILEAGKQPRMAQTVTSTALDRIQVHALHAVVVYEGKA
                     HLPDSHPIETPQGTIHITVEKTREPITLFLMSYNPVDWRISAVPGARIGSVILRGYYR
                     QTIRGLDKTVPVNTASTEDDNWDDLFPRTLVTSRDGLIDFKAYVRAMAGVEPATVQSL
                     HTGDSIVIDGKTTLEFPAPIEVPITQPVVFKSESESSARFRGRNMISPDRLTVGYGMS
                     GASTESIATMPHSSGRWYAEFIVHAGADNAVPISWTNVGIRSTANESGGLTSHGGQLA
                     YGVKQYLRNVGGLRDKDVIGLAMDLTRGHLYVRHNGVWVGGSPDSGNPILQIVRDRQY
                     VVAVEVGSPGGDYSKSDTWSGNFGATPFRHPVPDGFLPYEGRRPK"
     gene            complement(10415..>10878)
                     /locus_tag="PK589_16900"
     CDS             complement(10415..>10878)
                     /locus_tag="PK589_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNJ21344.1"
                     /translation="MTNIIDGIRSLTVPMVVLGMWCLLPVYAIAVTGDEATHEADHAL
                     DAEIIELPEVHVHGLPLNKDQQVGPVPKSTPWPAIPSSLEGKPLDDWMKARLLVSKEA
                     QVTVVVLESAKHRELTIAGIIALNKWTFAPQLKGDDPVDGELTVRIHFRSQ"
BASE COUNT         2216 a         3334 c         3066 g         2262 t
ORIGIN      
        1 tgaaactgcc ggaaggcgcc tcgctcgacc gtacggatgc ggtgttacgg caggtggtcg
       61 cgatcagcaa gaccgtggac ggcatcgccc atgtggtagc catgaccggc ctgaatccgt
      121 tgcagtttac gaacacgccg aattacggca tcgcctttct catcttgaag ccgttcgacg
      181 agcgccaccg cagcgccaag gaaatcagcg aggagatgaa tcaaaaaatc tccgccatca
      241 aggaaggcat caccttcgta ttgatgccgc cgccgattct cggactcggc aacggcgcgg
      301 ggtatggact cttcgtcgaa gaccgggctg gactgggctt cggggcgctg caaaatgcgg
      361 tcggcggcct gcagggagcc gtcatgcaga caccgggtat gggctacccg atctccggtt
      421 atcaggccaa tgtgccgcag ctcgacgccg aggtggaccg cgtcaagacc aagacacagg
      481 gcattgcgtt gacggatctt ttcgccacga tgcagatcta cctcggttca gcctatgtga
      541 acgacttcaa cctgttcggc cgcacctggc gcgtgtatgc gcaggcggac ggcgattttc
      601 gcggaaaggt cgaagacatt tccaatctca aaacgcgcaa tgcccgcggc gagatggttc
      661 cgatcgggtc gatggtgaag ttcagtcaga cgtacggtcc tgatccggtg ctgcgctaca
      721 acggctaccc tgccgccgat ttcatgggag aggctgatcc caccaagttt tcctcggcgc
      781 aagcgatgga cacgattcgc gacatcgccg ggaaggtgct gccgaacggc atgaccatcg
      841 aatggaccga tttgagcttt caggaagcgt cgcaaggcaa ggcggcgctg ctggtgtttc
      901 ccatcgccat tttgctggcc ttcctcgtac ttgccgctct gtatgaatcc tggaccctgc
      961 cgctcgcggt gatcctgatc gtgccgatgt gcatgctgtg cgcgctggcc ggcgtcaaac
     1021 tcaccggggg cgacaacaat acgtttgtgc aggtcggcct ggtggtgtta atgggtctgg
     1081 cgtgcaagaa cgcgatcctg atcgtcgaat tcgcgcgcga gctcgaatta cagggcaagg
     1141 gcattgttga ggcagccctc gaagcctgcc gcctccggct gcgccctatc gtgatgacgt
     1201 cggtcgcctt catcgccggc acggttccac tcgtgctgtc acatggcgct ggggcggaag
     1261 tgcgttccgc caccggcatc accgtattcg ccggcatgat cggcgtgact ctgttcggct
     1321 tgtttctgac acccgtgttt tacgtcggcc tgcgcatgct atccggacgc aagctgatca
     1381 ggcacgatga aggaatggac gtggcacggc cggcaggtca gttgagcgga gtcgagaacc
     1441 cgcattgagg cgggttaatt gacccgaact tttggtgcag tgcgcgccac cttattctcg
     1501 cccacatgcg atgccgctgg ctcgaatcca tatttgagca gaagcttttg aatccgggga
     1561 ctcatgaaga aatcggtgaa ttgttcggca gccgtgcgtg actcatcgcg gcaggtccac
     1621 actatcgcct gcccgaaccg gaccggcgta taggtacccc ccggcatctc atcgataata
     1681 cgcacctgtc cgctgttgtt tatcgcatct acgcggtaca ccagccccat ctctgcttct
     1741 tcccgtcgga tcatctcaat gacttcgtcg tgatgcgtgg ccgagatcag acggaaccgg
     1801 tctttatagg aaggatcaac cgctcgaaga gcccgtgcgg taatagggcc ggcgacagca
     1861 ctttcaaacg ccccgattgc aaccctggtt cttcgctcag acaacaggtc gtgaaaagaa
     1921 atcggcatgg cctgcgacgc cgccgacatg acgaacacca acgacgtttg cgcatagacc
     1981 tcgggcccgc cgttgagggt caatcccttt ttctgcaatt tctcaagatc ctccaccgcg
     2041 gatagcaaca catccacggg cgctccgctc tcgacttcac ggcggagggt ttgcgaggaa
     2101 ccgtacacga cgttgacggt ggccccgaac tcactttcga acatgggcac cacttcctga
     2161 aaggccgcct tcagactata ggccgcgccg atcgtcagat tttctgcctc tgcaggctcg
     2221 ccgatggcca gtcctcccac aagcccgact gccattacga gcgccttgct gaatctccgg
     2281 aatgtcttca ttacgtcaac ctcccccgtg ttccctctgc gcaggtcttc gcgaacattg
     2341 ccatacgcca gcttccgcac acgacgagat tgtcgcaaga ttagtaccag ccgcatccgt
     2401 cgtcccacga tctccgaaag ccggaacaat tgttgaggtt cttgtgaaat gacggagctt
     2461 tttgcatgac cgctgagatt tctcgtctgg caaaaccgtg agactttgca ctgtgcgcac
     2521 agtgcgataa tgccccttca agaatttgcc ttcccgttgt cgcaagacga cgcaatcaaa
     2581 ttcagtgatc gctccctcca aaacgtgcga atacacgacc attgtcgtcc gtcgtcacaa
     2641 caggcgcagg tcggcaagaa ccttgctcta tcccgtcatc aagaatcgca cactgctctt
     2701 cgtgttctaa tcatagatct gcaatccccg tattgcagga cgaggacttg ctgtatgaat
     2761 gaaatcgtcc tcattgccct ccgccgtccc tacaccttcg tggtgatgtc cattctcatc
     2821 gtgctgctcg gtagcatcac ggtgctccac atgccgaccg acgtgtttcc caatatcacg
     2881 atccccgtca cctcggtggt gtgggcatat gccggcctgc tgccgcagca ggtcgaagga
     2941 cgcatcacct atctcttcga gcgctttctc acttcgacgg tggagggcat caaatacatt
     3001 cacagtcact cgtattacgg cagcagcatc accaacattt tcctccagga cggagtggat
     3061 gtggggagag ccgaggcgga tatcacggct attgcgcaga ccgtcgtcaa ggcgctgcca
     3121 ccggatatct cgccacccat ggtcatgcgt ctcgcgccct cttccatccc tgtggcgatg
     3181 ctgcagatca gttccgaccg gctgaccccg gcggagctct acaatctggc ctacatgcga
     3241 atccggccct tactcgtgac cgtgccggga atcgtcctcc cccaccccta tggcggtcag
     3301 gacatgcagg tcatggtcaa tctcgaccag caaaagctgc ttgcccgcaa tctgacgccg
     3361 gccgatattc acaatgccct catgcagcag tatctggtgc tgccgtccgg tgatatcaaa
     3421 atcaagcaaa ccgattggat cgtccagacc aatgcttccc cgatgcagat cgaacatttc
     3481 agcgatatcc cggtcaagcg ggaagacaac gccttcatct atttgcgcga tgtggccact
     3541 gtccgcctca tgggccgggt gcagcagaac gcggtactgg tgaaaggcaa acagacagtt
     3601 atcatcgttg cgatgaagag cacggaggcc tcgaccctcg acgtggtcaa tgggatcaag
     3661 aagatgatcc cgcgcgcgga gcagatctct cccgagggcg tcgagatcaa actgctcgac
     3721 gacgcctcca ccttcgtgaa ggattcgatt gccgatgtgc tgcatgaaat ggcaaccgca
     3781 ggcgcactgg tcggcctgat tgtgctgctg ttgctcggat catggcgggc cacggtgatc
     3841 gtctggacgt caattcctct ctccatcctc accgccctca tcggtttgca ctggctcgat
     3901 gagacgatca acgtcatgac cttaggcgga ttggcactgg ccgtcggcat cctcgtcgat
     3961 gacgccaccg tgatgatcga gaatatcgac cggcatctcg aaatgggcaa gccgctcgag
     4021 caggccatcg tcgatgccgc gaatcaaatc gtggtgccga cattcgtcgc caccctctgc
     4081 attgcgattg tctggattcc gctgttcgaa ctcggcggcg tcgccggcta cctgttcaaa
     4141 cccatggcgg aagcgatcat cattgcgatg ctggcttcct tcatcctgtc gcgcacctta
     4201 gtgccgacca tggccaaata catgatgaag gggcaccatg tcgaagccgc ttgaacctgc
     4261 tgaacagccc ggtccggttg tctcaggcaa tatcttcgtt cggtttcaac aagggttcga
     4321 acgcgggttc aaccggttcc gtgagcgcta tgggctgctg ctcgaacggg ccatggcccg
     4381 gcgccgggcg tttgtcacgg tctgcctcgt gatcgccgtg gcctcgctgt cactcttctt
     4441 ctttctcggc cgcgactact ttcccgaaat cagatccggt gtcattcaga tgcatatgcg
     4501 ggccccgctc ggaacacgca tcgaggtatc cggacgtatc gcgacgctgg tctccaacag
     4561 tgtcgaagag ctgctggccg gtgaggtgga aaacatcgtg agcaattgtg gcttaccggt
     4621 gggagcgcat aacttggcgt tcattccgac gccgaccatc ggatcgcagg attgcgacct
     4681 cacgattctg ttgagggatg aaaagtcgcc ggtgtgggag taccggcgca ttctccgcaa
     4741 agggttgacg gagcgttacc ctggtacaga gtttacgttc cagccgtccg acctgactgc
     4801 caagatcctg aacttcggcg caccggcccc gatcgacgtt cagatcaagg gtccggacat
     4861 gcatcccaac tatgagttca cccgcaaact ggccggcaga ctacgccaga tccccggcgc
     4921 gaccgacgtg gtcatccaac agaccatgcg gacgccgacg ctcatggtcg aaggcaaccg
     4981 caccttcggt ctggccgtca accggactca aaaagaaatg gcgtccaacc tcctgatgac
     5041 gaccgccggc agccaacaga tcgaccaggt gtattggctc gatcccaaga ccggcatgtc
     5101 ttatctgatc aacgtgtata cgccgcaagc gcaggtcaat agcgttaaca gcctcagaac
     5161 cattcccgtt gaaccttcga atgatgtgtc ggacaacaat catgatgtgc aattgctcgg
     5221 caatctcgcc gacctctcgg ccgagggcac accgggcgtg atcacccacg cgaacatcat
     5281 gccgctgttc gatatctacg ttggcacgga agggcgcgac ctcggcggcg tattggcgga
     5341 tgtcgaaacc gtcgccaaga gcatggagca cgagttgccg cgcagcgccg ccatcgagat
     5401 ccatggccag gcttcgctga tgcacgacgc gtatttcgag ttgatcttcg gactactggc
     5461 cgcgatcgtg ctggtctatt tgctgattgt cgtcaacttt cagtcctggc tcgatccgtt
     5521 catcatcatc accgccttgc ctggtgcgtt ggccggtatc gcgtgggcct tgttcctgac
     5581 gcatacgcgc ctctcggaac cggcgctgac gggagccatc atgaccatgg gaacagcgac
     5641 ggccaattcc attctggtcg tctcctacgc gcgcgaacgg ctcgaaacgc atggcgaagc
     5701 gctgcgggcg gccattgaag ccggcacgac ccgcttccgc ccggtactca tgaccgcttc
     5761 ggccatgatc ttcggcatgg tccccatggc gacaggatat tctcagaatg ccccgctcgg
     5821 ccgtgccgtg atcggcggcc tgctcgtggc cacgatcttc accttgtttt tcgtgccgtg
     5881 cgtctatgcc attctgtatg acgggcgaag cgttcccgca aaggagcaac tctgatgctg
     5941 aagcgtctct ctccacgccc tggagtccta ctcggtattg tcctgcttct gttctatctc
     6001 agttatcgca tctacgagag caatgtcgac gccacaacct tgcgcaagca cacgctggag
     6061 gacacgatcc ccacagtggc catcacctac cccaagccgg tgccgccgac cgaaacgatc
     6121 accctgcccg gtaatatcca ggcctggttc gaagcgccga tctacgcgca ggtctccggc
     6181 tacgtcaaga tgtggtacaa ggactacggc gccctggtgc aaaagggcga catcctcgct
     6241 gaaatcaacg cgcctgccct ggatgcgcaa tatgcccagg cgaaggcgga tctcgccgca
     6301 gaacaagcca aatatgccct cgccgacttg acggcgaaac gctgggtcgc gctgcgcaaa
     6361 aaccatgcgg tctccgaaca atccatctcc gtgcaggaag ccaatgccaa ggccgaagcc
     6421 gccagggtca aggcagcgga gcagaacgtg aagaatttcg aagcgctgat ccagttcaaa
     6481 accatcgtcg cgccctatga cggagtggtg accgtccgca acatcaatgt cggcgactac
     6541 atcaataaag aaggcaacat cagcaaccgg agctccatca ccaatttgtt taccgtcgcc
     6601 gatgtcagca tgctgcggct attcgtcaac gtaccggcag ccttcggagc gtttctcaag
     6661 ccgggactga cggctgacgt gacggtaccg caattacccg atcgtcattt caccgcgaaa
     6721 ttcctgacgg tcgcccgcgg gttcgatgtc agcacacgca cggccgtcac ggtcttcacg
     6781 atcgacaacg aagaccgggc gttgtggccg gggtcctatg ccaaggtcca tctcaccgcg
     6841 ccggtcgacc ggaacgtgct cactattcct tccaccgcgt tggtgttcca ggagcacggg
     6901 acgcaagtgg ccgtggtggc ggaggacgat cgtgtgcagc tgaaacccat caccgtgagc
     6961 aagctcatgg acaatgccgt cgaagtgtca ggagggattt ccagtcagga ccgcatcgtg
     7021 aacaacccga gcgccgcctt gctggaaggc gacaaggtcc gcgtcgtcac accggcgccc
     7081 gggtatgatc ttgtgggcac cgcgacgcca ctcgcgaagg aaccggcgtt gaaatgacct
     7141 gtttccgatc caggccagcc ccatgcgtat caggtgaacg agcggacgca cgccgattca
     7201 cagacctcgc ccgaaccgcc gcgctcctgc tggcgctcag cctgtcggcc tgcagtacct
     7261 ttcccgctgt cgatctctcc cctccatacg agccggctca attcgtggtc ccggcctcat
     7321 ggcatgggtc gagcccattc gtcgaagcga agccgtccga tggcgagttg cgtcaggact
     7381 ggtggactct cttcaaagat cccgtcctga acgggttgga agcccaagcg ttgacggcca
     7441 atcctgacct gcaggcggcc gctgaacggt tcgtgcaatc ccgcgatgtg atgatgaagg
     7501 cacgatcgca atatctgcct cgtgtcggct tgggctttga cgccaccgat aatcggcagt
     7561 ccctccaccg gctgtttcgc gcgcccgata gtccattgga agaatcgagc gttgtgggcg
     7621 gcgggctcgc atcctgggaa ccggatttct ggtcggcgct gcgcaacgcg acaaaggtag
     7681 agatctaccg ggcggaagag cgcgccgccg acttcggact cgcacggctg agcctgcagg
     7741 cggaggtggc ggccaactat tttgcgttgc ggggcctcga cgcgcaggat gccatctaca
     7801 cccaatcgat cgcgctgtac acccagtcgc tggaactcgt caacgcccag ttcatcggcc
     7861 agattgcatc ggcgctcgac gtggctcgcg tcgaatccct gttgttcagc accgagacga
     7921 ggcgtgccca aatccaaggg caacgccagg tgttggaaca agccatcgcc atcttgctca
     7981 acgtggcacc ggcctccttc acgatcgcgc cggtcaacga cctcaaaatg gccaccttca
     8041 cgatccccca aacacttccg tcaaccttat tggaacgccg gccggatatc gccgccatgg
     8101 aacggcgcat ggcgcaggcc aatcgcacca tcgggattgc gcgcgccgcg ttttatccca
     8161 atgtgtcgtt tcgactcggg ggcgggtttg aggacgaggc cttcaatctg ttctcgctcg
     8221 cgaacagttt ctggtcctac ggctccggcg tctcgctgcc gctgttccag ggtgggtatc
     8281 gccgggcgca attgcagcag tcctggtctg cctatcgtga aacagaagat cggtaccgat
     8341 cgaccgtact caacgccttc cgggaggtgg agaataatct gagtctgacc aaccggctga
     8401 cccttgcggc caataggcag gatgccgccg ttggagcgac cctcaagacg caagatctca
     8461 ctatggagct ctatcagggc ggtctggctt ccagcctcga gctcatttac gcccaggtcg
     8521 ccatgctgac cgcgcggatc gagtcggtgc agatcaaggc tgaattgctt cgcacttcgg
     8581 tggccctcat ccgctcgctc ggaggaggct ggaaccgcca gcaattgccg accgatgatg
     8641 agattcaacc cttcgggacc ttccaatacg tgaatctcga caagccacca tcagttcgcg
     8701 gcatcgacgt caacgcggag aataatcgcc ttcacaacga cctcaccaag aagcccgccg
     8761 ttcggtagta gctgtccatc acacacaaaa gccggtcctg attggtggag agcggctctc
     8821 gtggtatctc tgtttggaaa gagtacagcc cttcctgcca ccattgaggc acaatcatga
     8881 tgattcggaa cgttttcgga cttctcctcc cgcttgcact gacagcgcaa ctggccggct
     8941 acgctgcgct tgtcgttcat gctgcgaatg agaaacaggg cgctcagcac tatgagttgt
     9001 ccagaggggc aggagagaaa cgatccgagt gcgtgggaat ggtacgaatc ggtcccggta
     9061 tagcagccat ggatttggag gcaacccgca cggattcgga tgatgcgcag atttctctac
     9121 gagggaccag agactctttc gtgtgcgaag ccattcttca cccggctatg cgggccgttc
     9181 tcggacgcag cattgttcag ttccacaatg gcaccactct gcctctcgcg caggtattgc
     9241 gccagattct tgaagcgggt aagcagcccc gcatggcgca aactgtgacc tccaccgcgc
     9301 tcgataggat acaggttcac gcacttcatg ccgtggtggt ttacgagggg aaggcccatc
     9361 tccctgactc tcatcctatc gagactcccc agggaaccat tcacatcacg gtagagaaga
     9421 cgcgcgagcc gatcacgctc tttctcatgt catacaaccc tgtcgattgg aggatctccg
     9481 ccgtacccgg cgcgcgcata gggtcggtga ttctccgcgg ctattaccgt caaacgattc
     9541 gaggactgga caaaaccgtt cccgtcaata ccgcatccac tgaggatgac aactgggacg
     9601 acctgtttcc tcggaccctc gtgacatccc gagatggcct gatcgatttt aaagcctatg
     9661 tgcgcgcgat ggccggagtc gaacccgcga cggtccagag cctccacaca ggcgattcga
     9721 tcgtgatcga cggaaagacg acgctggagt ttccggctcc aatcgaggtg ccgatcacgc
     9781 aaccagtggt attcaagtcg gaaagcgaat cctcggcacg attccgcggg cggaacatga
     9841 tttctccgga tcgtctgact gtcggatatg gcatgagcgg cgcttcgact gaaagcatcg
     9901 ccacgatgcc gcacagcagc ggaagatggt acgccgaatt catcgtacat gccggcgccg
     9961 acaacgcggt cccgatctcg tggaccaacg tcggtatccg atcgacggcc aacgagtcag
    10021 gcggactgac ttcacatggc gggcaattgg catacggagt gaagcagtat cttcggaatg
    10081 tgggcgggct gcgcgacaaa gatgtgatag gactcgccat ggacttgacg cgaggccatc
    10141 tctatgttcg acacaatggc gtatgggttg gaggctcgcc cgacagcggc aatccaatcc
    10201 tgcagattgt cagggaccga cagtatgttg tcgcagtaga ggtcggctcg cccggtgggg
    10261 attacagtaa gtcggacacg tggagcggca attttggggc gacacccttc cggcatcctg
    10321 ttccagatgg gtttttgcct tacgaaggtc gccgcccgaa gtagactgtg ctcgtcccgc
    10381 aagggaccct tgcggctcgc ttgtcgagac gtatctattg cgatcgaaag tgaatgcgca
    10441 cggtgagttc cccgtcgact ggatcgtcgc ccttgagctg aggggcgaag gtccatttgt
    10501 tgagcgcgat gatgccggca atcgtgagct cgcgatgttt ggccgattcc aacacgacca
    10561 ccgtcacctg cgcttctttt gacacgagca agcgcgcctt catccaatcg tcgagaggtt
    10621 tcccttccag cgacgaggga atggccggcc atggtgtcga tttcggcacc ggtcccacct
    10681 gctgatcctt gttcagcggc aatccgtgca catgcacttc gggcagctcg atgatctccg
    10741 catccagcgc atgatccgcc tcatgcgtcg cctcatcacc tgttactgcg atggcgtata
    10801 caggcagcag gcaccacatg ccgagcacta ccatgggtac tgtcagtgat cgaattccat
    10861 cgatgatgtt cgtcatat
//
LOCUS       DAOQDS010000096        10623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_3103_length_10623_cov_18.148656, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000096 DAOQDS010000000
VERSION     DAOQDS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3103_length_10623_cov_18.148656"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PK589_16905"
     CDS             <1..408
                     /locus_tag="PK589_16905"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ21345.1"
                     /translation="ACDPATLTVGQLMQDALFTCTPRTDALTIGRLMTKQNFGGLPVI
                     GEHGDLVGLVTEYDLLQAMIEGRDLRKVLAADIMTTHPLTAREDMRLEEVANLFQDRY
                     VTRLPVVRGALLVGIVARRDLLHGYMKASEYWS"
     gene            876..1739
                     /locus_tag="PK589_16910"
     CDS             876..1739
                     /locus_tag="PK589_16910"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNJ21346.1"
                     /translation="MEKRTKLAEIVRNQQDTILKDWVDFQLNAITVRRDLMKDSELRE
                     QSREFLNLFTQAAQTGSDVSEAIWKPVKDFLGQITRSRVLLGFTPSETATFIFSLKQP
                     LFDVIRAAVKDPKDLVSDMWTTTTLLDKLGLYVMELHQQNREEVIHRQQEELLELSTP
                     VVQLFENILALPLIGTLDSARTQVVMENLLQRIVETGATISIIDITGVPTVDTLVAQH
                     LLKTVAAARLMGADCIISGIRPQIAQTIVHLGVDLSEVTTKATLADAFMIALQRTGAT
                     VTKSAATRSKG"
     gene            1742..2131
                     /locus_tag="PK589_16915"
     CDS             1742..2131
                     /locus_tag="PK589_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNJ21347.1"
                     /translation="MERIPILKMGNFLLVTVQVDMHDRLAMTLQDDLTGRIVKDRAHG
                     VLIDISSLEIVDSFIGRMIGNIASMARVLDAETVVVGMRPAVAITLVELGLSLPGVRT
                     ALNVDQGVELLKESIEERAQGNGHVAG"
     gene            2112..2516
                     /locus_tag="PK589_16920"
     CDS             2112..2516
                     /locus_tag="PK589_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010883997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HNJ21348.1"
                     /translation="MATLRDEIYPIRSSDDIVKVRQITREWALMQTFSLVDQTKIVTA
                     ASELARNTLEHGRGGEVRIQALQEGPRRGLRLTFTDQGPGIPDLAKALQDGYTTGNGM
                     GLGLSGSKRLMNDFDIQTEVGKGTTVMIARWK"
     gene            2522..3556
                     /locus_tag="PK589_16925"
     CDS             2522..3556
                     /locus_tag="PK589_16925"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein/SpoIIE family protein
                     phosphatase"
                     /protein_id="HNJ21349.1"
                     /translation="MIKEQQMAGDQVQVPVGESTRVAEARRKAVELAGRIGLDETKTG
                     TCAVIASEMGTNLVKHATDGEMFVRSVTQGTMQGIELVAIDRGPGMSNVSHALQDGTS
                     TAGSSGNGLGAMMRMAAEFDIHSLPGRGTSIVARVWNGPVPVRQASVMKTGVIHLAKP
                     GETLSGDAWYSQCTGHRTLCAVVDGLGHGALAAQASQLAVETLRAHAQASLTEQVDLA
                     HRALRPTRGAALGIAEILHDQQLVKFVGIGNIMASICQVGSSRNMVSQNGILGHQIRR
                     VTEFQYPWPESAMLVMCSDGINTHWDINGYAGLAARDPSLIAATLYRDFSRGRDDTTV
                     VVLKKRTADG"
     gene            3629..5806
                     /locus_tag="PK589_16930"
     CDS             3629..5806
                     /locus_tag="PK589_16930"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ21350.1"
                     /translation="MSYAIINVALKYEDDVVAARQRARQIAGLIGFDAQDQTRIATAV
                     SEIARNAFRYARNGAVEFLIEGQTAPQVLLIRVTDSGPGIADLKLILEGRYQSDTGMG
                     LGIVGAKRLMDQCEIDSAVGKGTRVLLKKVFPSKTRALGAKELSSLVDQLIQTPAQNA
                     QEELRQQNHELLRALDELRLRQDELVRMNQELEDTNRGVVALYAELDEKADHLRRADS
                     MKTRFLSNMSHEFRTPLSSILALSGLLLDRSDGELTAEQDKQVRFVRKAAESLLELVN
                     DLLDLAKIEAGKVDVRPVEFEVKTMFSALRGMLKPLLAGSSVNLHFEEAEDLPTLYTD
                     EGKVSQILRNFISNALKFTERGEIRVTASVDAERRNIAIAVADTGIGIHPADQARIFE
                     EFTQLDSPIQRKVKGTGLGLPLCKKLALLLGGQVTVDSQVGSGSVFTLTMPLYHEKAL
                     DNMLKPGGEAERELDPARLPVLIVEDHPETRLVYEKYLRNSRYQALPSSGLREARRLL
                     YQVRPAAIVLDLLLCGEDSWQWLAHLKSDERTREIPILIVTTVEDRAKAFMLGADAYQ
                     VKPIEAPVLIAELDRLTKKRDHVEPPAPDRPRIMIIDDEESFRYILRKCLSNIDCDVT
                     ESGDGMEGIRMCRELRPDLIFLDLNMPGRSGWQFLQDLAASEKLAGTPVVIVTSQSLS
                     NEARTLLEQSTRGIVMKSELSEEAILHVVGELIPEAFARHHQS"
     gene            5828..8764
                     /locus_tag="PK589_16935"
     CDS             5828..8764
                     /locus_tag="PK589_16935"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ21351.1"
                     /translation="MSTIFQHIILNVDDDEGARYAKTKIFERAGYRVVEAATGADTLR
                     LVQEQQPHLVLLDIGLPDISGIEICRLIKKNPATEHMMVLQVSASCVRPQERAMGLDG
                     GADVYLSEPVEASELIGAAKALLRLYDREQESRVLLKELTERERFIKSMVDAAPSTVY
                     LYDLREQRNLYTNARSGATLGYASDELQFVGRETLQSWIHPDDRPLVMAQLAQLPDQA
                     DGQVLEFECRVQHRSKEWRWFLCRNVVFSRDENGQPLQILGTALDITDRKQQDAILRR
                     GKEQQALLLDISRLILEMPVDRAAWTRAIYEKVAPHLDVDLCLNFLLDQKTKGLQLLV
                     GLGVPDAMLAKAQRLDPGQAFCGYVVDAGTSLVADAELISTDPRGKFVREMGARAYAC
                     HPLKASTGEILGTLSFGTSRKERFTEEEIAFFQTLCHLVALAWERHSVSSALGESEMR
                     LKAIVDSALDGIITIDDQGTIHRVNPAVGRLFGYPADALLGRHITLLLPSLYSVEGAD
                     TPGVDQPRDDSGVSGLRREMLGVRMDRSSFPVELAISENHLDDAVTYTLLLRDITERK
                     KVQEQTRQWTIELEQRVSERTEELLLSRTRLRALASDLTLTEQRERQRLATELHDYLA
                     QLLVFGRMKLSQAKRGNLGPATTLVKELDDMLDEALTYTRSLVAQLSPPVLREFGLVV
                     AIKWLAEQMRRHEMTVTVQCEADLAPLREDQAVLIFQSARELLINVSKHAKTSQASIS
                     VWIESDMLHLCVGDEGVGFDLASTTNIAAPMFGLFSIRERMEALGGRMAVESAPGRGT
                     KITLVVPYVGGSTATDVHSRAGNALASSQVSEPEAVSVLDALAVSPGPQSAHSSRTRV
                     LLVDDHAMLRQGVRTVLEGYPDIEIVGEANNGEQALLLAQSLQPKIVIMDINMPGMDG
                     IEATRRLKLEQPETLVIGLSVHNDWQIEEAMRAAGAVSFLPKDAAIEQLHETIQVAIR
                     AAARTA"
     gene            complement(8795..10021)
                     /locus_tag="PK589_16940"
     CDS             complement(8795..10021)
                     /locus_tag="PK589_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ21352.1"
                     /translation="MPSSHSRHKLPSGIWILGFVSLLMDISSEMIHSLLPLFMVTTLG
                     AGTIAVGLVEGLAESLALVVKIFSGTLSDYLGKRKGLALCGYTIGALTKPLFAWAPDI
                     GTLLTARLLDRLGKGIRGAPRDALVADIAPPQLRGAAFGLRQSLDTVGAFLGPLLATG
                     FMLLWADNFRAVFWVAVIPGMLAVALLMVGIQEPVSSSSSRRANPITRANFRRLSPAF
                     WWVVGIGSIFTLARFSEAFLVLRAQQGGIPLAFIPLVMVAMNLVYASSAYPFGKLSDR
                     INHKTMLTWGLLVLIVADVVLATTNHWGVILGGVALWGIHMGMTQGLLAAMVADQAPP
                     DLRGTAYGCFNLASGLAMLMASIVAGFLWDRLGAAFTFYAGALFSSIAVIGIVVSPGT
                     APAPSAGHSLPPRSGE"
     gene            complement(10109..10528)
                     /locus_tag="PK589_16945"
     CDS             complement(10109..10528)
                     /locus_tag="PK589_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21353.1"
                     /translation="MNMKWTHCLPMLLALCLALPTASPAGSPLPAEPGDDFLIEQTFD
                     SVVGLLFRDYSLQGNGQVDYRTARHILGISYDDPASAEPDVALHPLFYWYDRNHDGQW
                     ELWIDREEEGHLVDVTRYDWRQGDDLLTLYPNLRNPT"
BASE COUNT         2305 a         2914 c         3289 g         2115 t
ORIGIN      
        1 gcttgtgatc ccgccacgct gacggtggga caactgatgc aggatgcctt gtttacctgc
       61 acgcctcgta cggatgcgct caccatcggc cgcctcatga cgaagcagaa tttcggtgga
      121 ttgcccgtga tcggggaaca cggcgacctg gtcggtctcg tgacggaata cgatctgctg
      181 caggcgatga tcgaaggccg ggatttgcgc aaggtcttgg cagccgacat catgaccacc
      241 catccattga ccgctcgcga ggacatgcga ttggaagaag tcgctaatct ctttcaagat
      301 cgctacgtga cgcgtctgcc ggttgtgcgg ggagcgctgc ttgttggtat cgtcgcgaga
      361 cgggacctgt tacatggata tatgaaggcg tcggaatact ggtcgtaacc ggatgggata
      421 gattctcttt tcctgtccca tctggagggg cgaagtgccg ccgaccgtat ccattctcct
      481 gctctacacc ttcgaacacc gtacctcacc aagattcaaa agcgcgttgc gcctgggcca
      541 tagcaggcat gaatgaacga acagtctgca ggagttcccc aacggtccat gggtagttat
      601 ccaaggcgcg gcatcgacat cccaccgttg attcggctca ctttctcctg ctcataggcc
      661 cccgcagccg ctcccgggcg gcgaaaaacc cccttgttgt tccctctgga agtccaaaac
      721 gattttcgat aggctgtgcc gaattgccag gggttttcct agtgggagat agaccagcgg
      781 ggcgcataca gttacccgcc cccgtttgtc attccagatg aagtcccgcc gtaattgcat
      841 gggtacaatt tgatgtgaga ggaggctgga agggcatgga gaagcgaacg aaactggcgg
      901 aaatagtcag gaatcaacaa gacaccattc tcaaagactg ggtggatttt caattgaacg
      961 ccatcaccgt gcgacgtgat ctcatgaagg acagtgagtt gcgggaacag tcgcgggaat
     1021 tcttgaacct cttcacccag gcggcgcaga ccggatcgga tgtcagtgaa gctatctgga
     1081 agcccgtcaa agacttcctg gggcagatta ccaggtctcg cgttctgctg ggctttactc
     1141 cctccgagac cgccacattt attttttctc tgaagcaacc gctgttcgat gtgatccgtg
     1201 ccgccgtcaa ggacccgaaa gatctcgtct cagatatgtg gaccacgacg accctcctcg
     1261 acaagctcgg gttatacgtg atggaactgc atcaacagaa ccgggaagag gtcattcatc
     1321 gccagcagga ggagctgttg gagttgtcga cgcccgtcgt ccagttgttt gagaacatcc
     1381 tcgccctgcc gctgatcggt accttggaca gcgcacggac ccaggtcgtc atggagaacc
     1441 tgttgcaacg gatcgttgaa accggcgcga cgatctccat catcgacatt accggcgtcc
     1501 cgaccgtcga tacattagtg gcgcaacatc tgttgaaaac cgtcgcggcg gccaggctca
     1561 tgggtgcgga ttgcatcatc agtggcattc gtccgcaaat tgcccaaacg atcgtgcatc
     1621 tgggtgtcga cctcagcgaa gtgacgacca aagccacgct cgccgatgcc tttatgattg
     1681 ccttgcagcg caccggggct actgtgacga agtctgctgc gaccaggtcg aaaggataac
     1741 catggagagg atcccgattt taaaaatggg gaatttcctc ctggtcaccg tccaggtgga
     1801 catgcacgat cgcctcgcca tgacgttaca ggatgatttg accggtcgaa tcgtcaaaga
     1861 ccgcgctcac ggagtcctca tcgacatttc gtcgcttgag atcgtggatt cgtttattgg
     1921 tcgcatgatc ggcaacatcg ccagcatggc gcgggtcttg gacgcggaga cggtagtcgt
     1981 cggcatgcgt ccggctgtcg ccattacgct ggtcgagtta gggctgtcgc tccccggtgt
     2041 gcgcaccgcg ctcaatgtcg atcagggcgt ggaactgctg aaagagtcca ttgaagagag
     2101 agctcaaggg aatggccacg ttgcgggatg agatctatcc catccgctct tcggacgaca
     2161 tcgtgaaggt ccgtcaaatc acgagggaat gggcgctgat gcagaccttc tccctcgtcg
     2221 atcagacgaa gatcgtgacg gccgccagtg agctcgcgcg caatacgctc gagcatggcc
     2281 gtggcggcga ggtgcgtatt caagcgctgc aggaaggccc ccgccggggg ctccgactca
     2341 cgtttaccga ccaggggccg ggaattcccg atcttgccaa ggccttgcag gatggataca
     2401 cgacgggaaa cggcatggga ttgggcctga gtggctcgaa gcgcctcatg aacgacttcg
     2461 atatccaaac tgaggtcggt aaggggacaa cagtcatgat tgcgcgttgg aagtgagagc
     2521 gatgatcaag gaacaacaga tggcgggcga tcaggtgcag gtgccggtgg gcgaatccac
     2581 ccgggtcgcc gaggcgcgcc gcaaggcggt cgaattggcc ggacggatcg ggttggacga
     2641 aacgaagaca gggacctgcg cggtgatcgc gtctgagatg ggcacgaacc tcgtcaagca
     2701 tgcgacagac ggggaaatgt ttgtgcggag cgtgacgcag gggacgatgc agggcattga
     2761 gctcgtggcg atcgaccgag gccccgggat gtcgaatgtg agtcacgcat tacaagacgg
     2821 gacgtctacg gccggaagca gcgggaacgg tttaggcgcc atgatgcgga tggcagccga
     2881 attcgacatt cattccctgc ccgggcgggg gacgagcatc gtggcccgcg tctggaatgg
     2941 accggtgcca gttcgccagg cctcagtcat gaagacgggc gtcattcacc tagccaagcc
     3001 gggcgagact ctttccggtg atgcctggta ttcccagtgc acgggacatc gcacgctctg
     3061 cgcggtcgtg gatgggttgg gccacggagc cctggcggcg caggcgtcac agctggcagt
     3121 ggaaaccctg cgcgcccatg cgcaggcctc tctgaccgag caagtagatc ttgcgcaccg
     3181 cgcgcttcgg ccgacaaggg gcgcagccct tggcattgca gaaattcttc atgatcaaca
     3241 gcttgtcaaa tttgtcggta tcggaaatat catggcgtcc atatgccagg tgggaagctc
     3301 cagaaacatg gtctcgcaga atggcattct ggggcaccag atcagacgcg tgacggagtt
     3361 tcagtatccc tggcccgaat cggcgatgct ggtgatgtgt tcggacggca ttaacacgca
     3421 ttgggatatc aacggctatg ccgggctggc tgcacgcgat ccgagtctga tcgccgccac
     3481 gctctaccgc gatttttccc gcggccgcga cgatacgacg gtagtcgtgc tcaagaagcg
     3541 cacggcggat gggtgagatg cgcctggagt gtcccgcgcg aatctgaagg aatgacagga
     3601 tgggttccgc ctcgaaaggt cacacgcgat gagttatgcg atcatcaatg tcgcgctgaa
     3661 gtacgaagat gatgtcgtgg ctgcgcgtca gcgggccagg cagattgccg ggttgatcgg
     3721 ctttgatgcg caagatcaga cacgcattgc aacggcggtg tccgaaatcg cgcggaatgc
     3781 ctttcgttat gccaggaatg gggcggtgga gtttctgatc gaagggcaga ccgctccgca
     3841 ggtcttgttg attcgtgtga cggatagcgg gcccggcatc gccgatctga aactcatttt
     3901 ggagggacgg tatcagtctg ataccggaat ggggctgggt atcgttggtg ccaaacgttt
     3961 gatggaccaa tgcgaaatcg acagtgccgt ggggaaaggc acgagggtgc tgctgaaaaa
     4021 ggtctttccg tcgaagacca gagcattggg cgcgaaagaa ctctcgagcc tggtggatca
     4081 attgatccaa acgcccgccc agaatgcgca ggaggagtta cggcagcaaa accatgaact
     4141 cttgcgggcc cttgatgaat tgcgtctccg tcaggatgaa ctggttcgta tgaatcagga
     4201 actggaggat accaatcgtg gcgtcgtcgc tctctacgcc gagttggatg aaaaggcgga
     4261 ccacctccgt cgggcggaca gtatgaagac gcggttcttg tccaacatga gccatgagtt
     4321 ccgaacgccc ttgagttcga tcctggcctt gtcgggactg ctgttggacc ggtccgatgg
     4381 agaactgacg gccgaacagg acaagcaggt gcgatttgtg cgcaaggctg cggaaagcct
     4441 tctggagctg gtcaacgatc tcttggacct cgcaaaaatc gaagccggca aggtcgatgt
     4501 gcgaccggtc gagtttgaag tcaaaacgat gttcagcgct ctcaggggca tgctgaagcc
     4561 cctgttggca ggctcgtcgg tgaatcttca cttcgaagaa gcggaggatc tcccgacgtt
     4621 gtacacggac gaagggaagg tctcgcagat cctgcggaac tttatttcga acgcgctcaa
     4681 gtttacggag cggggagaga ttcgcgtcac ggcgtccgtc gatgcggagc gccgcaatat
     4741 tgccatcgcg gtggctgata ccggcatcgg cattcacccg gctgatcaag ctcgaatttt
     4801 cgaagaattc acccagttgg atagcccgat tcagcgaaag gtgaaaggca cagggttggg
     4861 cctgccgctg tgtaaaaaac tggccctctt gctcggcggg caggtcacgg tcgatagtca
     4921 ggtcggcagc ggctcggtat tcacgttgac catgccgctc tatcacgaga aggcgttgga
     4981 caacatgctg aagccgggcg gcgaagccga gagggagctg gaccctgctc gacttcccgt
     5041 cctcattgtc gaggaccatc cggagacacg gttggtctat gaaaaatatc tgcggaacag
     5101 ccggtatcag gcgctgccca gtagcggcct gcgtgaagcc cgccggctcc tgtatcaagt
     5161 gcggccggcg gcgatcgtgc tggatctttt gttatgcggc gaggatagtt ggcagtggct
     5221 ggcgcatttg aaatcggatg agcgcacgcg cgagatcccc attctcattg tcacgacagt
     5281 cgaggatcgg gccaaggcct tcatgctggg cgccgatgcc tatcaagtaa aaccgattga
     5341 ggcaccggtg ttgattgccg agttggatcg tctgacgaag aagcgggatc acgtggaacc
     5401 gcctgctccg gaccgtccgc gtatcatgat catcgacgac gaggagtcgt tcaggtatat
     5461 cctcaggaag tgcctttcga atatcgactg cgacgtcacg gaatccgggg acgggatgga
     5521 aggcatccgg atgtgtcgtg agctgcggcc cgacctgatc ttcctggatc tcaatatgcc
     5581 gggacgatcg ggctggcagt ttctgcagga tttggccgcc tcggaaaagt tggcgggcac
     5641 accggtcgtg attgtgacgt cgcaatccct ctcgaatgag gcgagaacgc tgctggagca
     5701 gtccacgcgg ggcattgtca tgaaaagcga gttatcggaa gaggccatac tgcacgtggt
     5761 cggtgagctg attcccgaag ccttcgcgcg ccatcatcag tcatgatctt ttggttgtaa
     5821 gaggaccgtg agcaccatct ttcagcacat cattctcaat gtcgacgatg acgaaggggc
     5881 gcgctacgcc aagaccaaaa tctttgagcg agccggctat cgtgtggtcg aagcggccac
     5941 cggcgcggat accttacggc ttgtgcagga gcaacagccg cacttggtgc tgctcgacat
     6001 tgggctgccg gatatcagcg gcattgaaat ctgtcgcctg atcaaaaaga accccgcgac
     6061 cgaacatatg atggtcctgc aggtttccgc ttcatgcgtg cgaccgcagg aacgggcgat
     6121 gggactcgat ggcggtgcgg acgtctatct gagcgagccc gtcgaagcca gcgagctgat
     6181 cggcgcggcc aaggcgctcc tacggctcta tgatcgagag caggagagcc gggttctgct
     6241 caaggaactc accgagcggg aacggttcat caaaagcatg gtcgatgcgg caccctccac
     6301 ggtctacctc tacgatctgc gtgaacagcg caacctgtac accaatgccc ggtccggtgc
     6361 taccctgggc tacgcgtcag atgaactgca gtttgtcggt cgagaaacct tgcagtcatg
     6421 gattcatccg gacgacaggc cgctggtgat ggcgcagctg gcgcaattgc cggaccaagc
     6481 ggatggccag gtgttggaat ttgagtgtcg ggtccagcat cgatcgaaag aatggcgctg
     6541 gtttctctgc cgcaacgtcg tgttctcgag agacgagaat gggcaaccgt tgcaaatcct
     6601 gggcacggcc ctcgacatta ccgatcgcaa gcaacaagac gccattctgc gtcgcgggaa
     6661 agagcagcaa gcgctgttgc ttgacatcag ccgcttaatc ctggaaatgc cggtcgatcg
     6721 ggcggcctgg accagggcca tttacgaaaa ggtcgcgccg catctcgatg tggatctctg
     6781 cctgaacttt ctgctcgatc agaaaacaaa aggcctgcag ctgctggtcg gattgggagt
     6841 gcctgacgcg atgctggcga aggcgcaacg gctcgaccct gggcaagcct tttgcggata
     6901 tgtcgtcgat gcgggaacgt ctctcgtcgc cgacgccgag ttgatatcaa ccgatccgcg
     6961 cggaaaattc gttcgggaga tgggcgcgcg tgcctatgcc tgccatccgt tgaaagccag
     7021 cactggagag atcctgggaa cattgtcctt cgggaccagc cggaaagaac gatttaccga
     7081 agaagaaatc gcatttttcc aaacgctctg ccacctggtg gcgctggcat gggagcgcca
     7141 tagcgtctcc tccgcactgg gtgaaagcga aatgcggctc aaggcgatcg tcgacagtgc
     7201 tctggacggc atcatcacca tcgacgatca gggcaccatc catcgtgtca atccggctgt
     7261 gggacggctc ttcggatatc ccgctgacgc gctgctcgga cgccatatca cgttgctctt
     7321 gccaagtctg tattccgttg aaggggcaga cactccgggc gtcgatcaac cgcgcgatga
     7381 ctcaggggtg agcggactca gacgagagat gctgggcgtt cgaatggatc ggtccagttt
     7441 cccggtggaa ttggccatca gtgaaaacca tctggatgat gcggtcacct acaccctgtt
     7501 gttacgcgac atcacggaac gaaagaaggt tcaagagcaa actcggcaat ggacgatcga
     7561 acttgaacag cgggtgtccg aacggacgga ggaattgctg ctgtcccgca cgcgcctgcg
     7621 ggcactcgca tccgatctca ccttgaccga gcagcgagag cgtcaacgtc tcgcgactga
     7681 attgcacgac tacctggcgc aactgctggt ctttggtcgt atgaagctta gtcaagccaa
     7741 acgagggaac ctcggtccgg cgacaacctt ggtgaaggaa ttagacgata tgctggacga
     7801 ggcgttgacg tacaccaggt ctttggtggc gcaattgagt cctccggttc tgcgcgagtt
     7861 cggattggtg gtcgcgatca aatggctggc ggaacagatg cgtcggcatg aaatgaccgt
     7921 caccgtgcaa tgcgaagccg atcttgcgcc gctacgcgag gatcaggcgg tgttgatctt
     7981 ccagtcggcc cgcgaactgt tgattaatgt gtcgaagcac gcgaagactt cgcaggcatc
     8041 gatctccgtg tggatcgaga gcgatatgct ccatctgtgc gtcggggacg agggtgtcgg
     8101 ctttgacctg gcgagcacga cgaacattgc cgcgcccatg tttggcctct tcagtatccg
     8161 cgaacgtatg gaagcgttgg ggggacgcat ggccgtcgaa tctgctcccg gccgtggtac
     8221 gaagattacg ctggtcgttc cctacgtggg aggatcaacc gcgacggatg ttcactcccg
     8281 ggccggcaac gcgctggcct cctcgcaagt gtctgagcca gaagcagtct ccgtgctcga
     8341 cgccctggct gtgtcgcccg ggccgcaatc ggcccattcc tcccggaccc gcgtgctgtt
     8401 ggtggatgac catgccatgc tgcgccaagg agtccgaacg gttctcgaag ggtacccgga
     8461 tatcgagatc gtgggcgagg ccaataatgg agaacaggcg ctgctgctcg cacagtccct
     8521 gcaaccgaaa atcgtcatca tggatattaa tatgccgggc atggacggca tcgaggcgac
     8581 acgccggctc aagctcgagc agcctgagac gttggtgata gggctgtcgg tacacaatga
     8641 ctggcagatc gaagaagcga tgcgagcagc gggagcggta tcatttctgc cgaaggatgc
     8701 tgccatcgaa cagttgcatg aaacgattca ggtcgccatt cgcgcagctg ccagaacggc
     8761 gtgaaggccg tgggtggtct gaagcaatcg cgcgtcactc gccactgcgt ggtggaaggg
     8821 aatggccggc agagggggct ggagcagtgc cgggagaaac gactattccg atgacagcga
     8881 tgctggaaaa caatgccccc gcataaaagg tgaatgccgc ccccaatcga tcccacaaga
     8941 atcccgccac aatgctcgcc atgagcatgg ccagtccgct ggccaggttg aaacatccat
     9001 acgcggtacc tctcaagtca ggcggcgcct ggtccgccac catcgcggca agtaggccct
     9061 gcgtcattcc catgtgaatg ccccacagcg ccactcctcc caatatcacg ccccaatgat
     9121 tggtggtcgc cagcaccaca tcggcgacaa tcaacaccag caagccccat gtgagcatcg
     9181 ttttgtgatt gatccggtcc gaaagcttgc cgaaggggta ggcagaggag gcatacacca
     9241 gattcatcgc gaccatcacg aggggaatga aggcgagagg gatgccgccc tgctgtgcgc
     9301 gcagtaccag aaacgcctcg ctaaagcgcg ccagcgtaaa gatggagccg attcccacca
     9361 cccaccagaa ggccggactg agacgcctga agttcgcacg agtgatcgga ttagctcggc
     9421 gggaggatga cgaagagacg ggctcttgta tgccgaccat gagaagcgcg acggccagca
     9481 tgcctggtat gacggctacc cagaagactg cgcggaaatt gtcggcccac agcagcatga
     9541 atccggtagc gagcaaagga ccgagaaagg cgcccaccgt gtccaatgat tgccggaggc
     9601 cgaacgcggc tccccgtagc tgcggtggtg cgatatcggc caccagcgcg tcgcgaggcg
     9661 cgccccggat gccctttccc agtcgatcca gcaagcgagc cgtcaggagc gtgccgatgt
     9721 ccggtgccca ggcgaagagt ggtttggtaa gggctccaat cgtatacccg caaagggcca
     9781 atcccttccg tttgcccaga tagtcgctca atgtgcctga gaagatcttc accacgaggg
     9841 cgagggactc ggccaaccct tccaccagcc ccaccgcgat ggtccccgcg ccgagtgttg
     9901 tgaccatgaa caacggcagc aggctgtgga tcatttccga agaaatgtcc atcaacagac
     9961 tgacgaagcc caggatccag atacccgaag gcagcttgtg acgcgagtga gaggaaggca
    10021 tgggcataac tatcggcgtc gtgccgtgac ggttgagccg atctgggtgc tcggcaggac
    10081 agattatagg aattgccggg tatggctgtc atgtgggatt ccgcagattc gggtaaagcg
    10141 tcaacagatc gtcgccctgc ctccagtcat accgtgtgac atcgaccaga tggccttcct
    10201 cttcgcgatc gatccataac tcccactggc cgtcgtgatt gcgatcgtac cagtaaaaga
    10261 gtgggtgcaa cgctacatcc ggttctgctg aagcgggatc gtcgtacgag atgcccagga
    10321 tgtgacgcgc tgtacgatag tctacctggc cattcccttg gagcgagtaa tcgcgaaaca
    10381 ataagccgac gaccgaatca aatgtctgtt cgatgaggaa gtcatctccc ggttcagctg
    10441 gaagtggaga tccggcagga gaggcggtcg gcaacgcgag gcagagggcc aacaacattg
    10501 ggagacaatg agtccatttc atgttcatcg catcctccct tcgccgcgta aatctgtgcc
    10561 ttcctcatct ttgcgctgct tccgtgtccg gcaccctgga aatgtgccta gttcagccgg
    10621 ttc
//
LOCUS       DAOQDS010000097        10505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_3159_length_10505_cov_20.199522, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000097 DAOQDS010000000
VERSION     DAOQDS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10505
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3159_length_10505_cov_20.199522"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..2103
                     /gene="oadA"
                     /locus_tag="PK589_16950"
     CDS             106..2103
                     /gene="oadA"
                     /locus_tag="PK589_16950"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HNJ21354.1"
                     /translation="MAIKKTKKAVSKKSPSKKSAPAKPTKAVRPAATRTVAAAPSVSS
                     TFNVVPAPAKKLQLTEVALRDGHQCLLATRMRTEDMLPIAQKLDSVGFWSLEVWGGAT
                     FDTCLRFLKEDPWERLRALRAAMPKTKLQMLLRGQNLVGYRHYADDVLDKFIERSAAN
                     GIDVFRIFDALNDVRNLERAIREVKACEKHVEAAISYTTSPVHRLDGFVTMGKRLEDL
                     GADTICIKDMAGLLAPVDAYRLVKSLKAAVRVPIHLHSHYTSGMGTMTALMAVMAGLD
                     LLDTSISPLAGGASHPPTESMVAALRGTPYDSQLDLEDLQPIAEHFRTVRRKYRQFES
                     DFTGVDAEILTSQIPGGMLSNLAAQLAEQNALDRMKEVMDEIPRVRKDMGYPPLVTPT
                     SQIVGTQATLNVLTGEQGERYKVITTETKNYFLGLYGRAPGPLDKEIMARAIGDEEPV
                     KGRPADRLEAEFEKLKKDMPESATTLEDQLSFALFPAIARDFFEAREQGALQPEPLEP
                     TDTKGPAVAHDLHLAPAEFNITVHGENYHVVVSGSGRTTDGRKPYYIRVNDRLQEVSL
                     EPLQEVLAGVPESPEASSTSKPKRPRPTKPGDVAPPMPGRVVKVLVTDGAQVKTGDPL
                     LIIEAMKMESQVPAPMDGRVAAILVAEGDNVKIDETVIQLE"
     gene            2107..3069
                     /locus_tag="PK589_16955"
     CDS             2107..3069
                     /locus_tag="PK589_16955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ21355.1"
                     /translation="MPRPASLVRFVAPAPHHLATWFLASLLMSGCAARPDVPPWFDAI
                     QRLPVHTISVTGHRIAYLDEGEGPTLILLHGYGGSMWQWEYQQPLTKHFRVITPDLLG
                     SGLSEKPDIEYRPSALIESIRGLMDGLGLQTATVIGNSMGGGVAIGMALTYPERVDRL
                     VLIDTLPDHVRERLVSPLMQRAINTHVPAWMARFWASLFGSRTMEAVLKEVVYDHSLI
                     TPLVLERSNRNRQREDMIGPLLSLRDSLPLWEQLYAPRLTAIRQSTLILWGEHDRLFP
                     PQVGQDLQAMIPHSRLIVIPEAGHIPQWERPQAVNRHIMEFLQP"
     gene            3137..3823
                     /gene="tpx"
                     /locus_tag="PK589_16960"
     CDS             3137..3823
                     /gene="tpx"
                     /locus_tag="PK589_16960"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNJ21356.1"
                     /translation="MRSMSAPVLSGVLVPRLLAGLLCLGLSACGTLPGTGKGGFSYKD
                     MPIADGSAVAGEGNNILFQGKPLMLTGMGIKVGDKLRDVKLAQIDLSMIPINDTKGKG
                     KVRIISIVPSLDTKVCEQQTHYLSEKNMGLDRMVELITISIDTPFAQKRFAEEAKISN
                     VTFLSDFRAADFGKAHGLLLKDLHLLSRALLVVDKDNKVRYLQITPELAQLPDLEDAF
                     RFARKLVTAS"
     gene            3854..5257
                     /gene="sthA"
                     /locus_tag="PK589_16965"
     CDS             3854..5257
                     /gene="sthA"
                     /locus_tag="PK589_16965"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNJ21357.1"
                     /translation="MAHYDLLVIGTGPAGQKAAIQAAKLGKKAGIVERTRVVGGVCTN
                     TGTIPSKSLREAALYLSGFHQRSLYGASYRVKQDITMADLTFRSNHVINREIEIIQNQ
                     MTRNNIDLWYGTASFVDPHRVRVERADDHVEHTADFIVIACGTVPARPAHIPFDDQTI
                     IDTDGLLKLKDLPKSITIIGGGVIGAEYASILATMGLHVTLIERRPRLLEFVDQETIE
                     ALQYHMRSIGVVLRFNEEVVSVEPISKELVIVRLKSGKEIAASTVLYSVGRTGASATL
                     NLEAIGLAADERGRLTVNEHYQTSIPHIYAAGDIIGFPALASTSMQQGRHAACHAFGV
                     PYQTQSDLMPYGIYSIPEISMVGRNEDDLTKHGVPYAVGIARYREIARGQIIGDEIGM
                     LKLLFHNKTRQLLGVHAIGDGATELIHIGQTVMAFQGQIDYFIDAVFNYPTLAECYRV
                     AALDGINQLPRPWTPRA"
     gene            5369..6037
                     /gene="tenA"
                     /locus_tag="PK589_16970"
     CDS             5369..6037
                     /gene="tenA"
                     /locus_tag="PK589_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HNJ21358.1"
                     /translation="MSFSNHLRKLAQPVWDAQLTHPFVLALGKGTLSERKFRYYILQD
                     ARFLADLARVFAAGSLRAPDSESALRFAKLAEETITVERSLHENYGKRWNLSPQEMLN
                     EPMAPTNYAYTRHMLAVAQAGTATEIAVVALPCAWIYCVVGKHFLKNGAPPAKHPYRD
                     WLMLYASPEFEAVQEWMRARVDTWARTAGHEEKKRMEQAFLISSKYEWMFWEMAWNEE
                     KWPV"
     gene            6379..7107
                     /locus_tag="PK589_16975"
     CDS             6379..7107
                     /locus_tag="PK589_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21359.1"
                     /translation="MPPHEEGKRKISFPNSNVSGGNIVVVLIENGKSPDQPYNARLGF
                     WLPPDSPYGNFQLKLRKLCERLDEANRRLIDTFDYWNQAMEQKTSVNLFQRHEYAIEQ
                     AVYLMRRVADELIAMIWCLHEWEKTGDYPRQIRIDCIGAALESTQQNLSKDLKVLFDP
                     HNPVMKALNEISNAFKHSFVHSDLTLIGRDEPCVYALGLAHNKLSSEMKFHNFSVSWL
                     VREFSAFYKSGMQWLASFSERHRK"
     gene            complement(7398..7556)
                     /locus_tag="PK589_16980"
     CDS             complement(7398..7556)
                     /locus_tag="PK589_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21360.1"
                     /translation="MPVYFLIALTILFCAIAIWASVTQESIPMRGEEEPDWDVEPLNQ
                     SQEYRKTG"
     gene            7807..8853
                     /locus_tag="PK589_16985"
     CDS             7807..8853
                     /locus_tag="PK589_16985"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNJ21361.1"
                     /translation="MTDAASTPTVLSSREIQLHLLGLVLLLGVLGLWYWFSTGARVVP
                     TLIELLKDDDPSTRIVAAEQLGQVGPAARSAVPQLLSQATQDGSQHANTTAATALKWI
                     DLTAARRVMTHFVPRLQDPDVQLRRTACAVIGSLGPIAKPAVPALLAVTHDADALIRR
                     NALIALASIGIPSLPIAAALVDGLRDSSSLVRQTAVAQFAFTVPPSPDAVAVLTPMLN
                     DPDKGIATLARTALDKPRAGDAAHIESLGMMLGHSSARDYALHQLAQLGPAAHDALPP
                     VLALLEDEQPLIRYLAAESIGGMGAVAKQALPALQRHQDSDLVVRAAIAEAVSAIEAG
                     AVVNAEPLSKELPR"
     gene            8850..9350
                     /locus_tag="PK589_16990"
     CDS             8850..9350
                     /locus_tag="PK589_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21362.1"
                     /translation="MTDIPRQTSPRLRMMARLGVWISLLGGLLLPWIIMLTVEILVRD
                     IPYPRAWRSFTLHLFAPGYNFFFVGVLTALPFVILALIILFHLGTAPANNALIAYRRA
                     LGLFSAGLSMALIAAWVHVDVLIHPDAQGALAYFYLPILLLMMLPFGYGLGRALAKIF
                     LPRLSG"
     gene            9426..10400
                     /locus_tag="PK589_16995"
     CDS             9426..10400
                     /locus_tag="PK589_16995"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNJ21363.1"
                     /translation="MIRLLPCLILLVLCHSTSLAASKYEDSVKELAEGVAAEAIKMKK
                     SRLALLDFVDSKGDVTPIGQFLAEELATHLLVAGELKVVDRKLLIATMEKHQLTHLDT
                     SQAKAAKKVAKALRADLYVTGAYLEIPEGIQVTAKLIGPYTVYPVGASRTIVPKSGPL
                     AALLKQAAAPAPATAAVVTDSAPVLSAHENDLYKFTILSVTRQDDLIGLDLVFTNRTA
                     HPVKIGCQLLDTYLVDEHGEQWKQNVAQNREGLCTRGMELNPKRQQRASLSFLATNKP
                     PTGPITLHYHETSPRADRIITLDGLKIASAPVVEPGAPAAQESTPPTP"
BASE COUNT         2277 a         3231 c         2873 g         2124 t
ORIGIN      
        1 tccgcagcaa tcgccgccta cgaaggactc tgacctctca gccaccaggc acacagcacc
       61 tcaaccctgt tacgcgagcg attccgccgc ctcgaggccg ccagtatggc aataaaaaag
      121 accaagaaag ccgtatcgaa aaagtctccg tccaagaaat ccgcgccggc caagccgacc
      181 aaagctgttc ggccggctgc cacacgcacc gtcgcagccg caccctctgt atcatcgacg
      241 ttcaacgtcg ttccggcacc ggcaaagaaa ctgcagctga cggaagtcgc cttgcgagac
      301 ggtcaccaat gtctcctcgc caccagaatg cgcaccgaag acatgctgcc catcgcgcag
      361 aagctggact cagtgggatt ctggtctttg gaagtctggg gcggcgcaac cttcgatacc
      421 tgcctgcgtt ttctcaaaga agatccgtgg gagcggctcc gcgcactgcg tgccgcgatg
      481 ccgaagacca agctgcagat gctcctgcgg gggcagaatc tcgtgggcta ccggcattat
      541 gccgacgacg tgctcgacaa attcatcgaa cgatcggccg ccaatggcat cgatgtgttc
      601 cgcatattcg atgcgctcaa cgatgtgcgt aacctggaac gggccatccg cgaagtcaaa
      661 gcctgcgaga aacacgtaga agcggcaatt tcctatacga cgagccccgt ccatcgattg
      721 gacggattcg tcacgatggg gaaacgcctg gaggacctcg gcgccgacac gatttgcatc
      781 aaggatatgg ccggacttct ggcgccggtg gatgcctacc ggttggtcaa gagtttgaaa
      841 gcagcggttc gtgttcccat tcacctccat tcccattaca cctcgggcat gggcaccatg
      901 acggctctga tggccgtgat ggcgggcttg gatcttctgg acacgtccat ttcgccgcta
      961 gcgggagggg cttcgcaccc gccgaccgaa tcgatggtcg cggcattacg cggcaccccc
     1021 tacgacagcc aattggatct ggaagacctc cagcccatcg cagagcattt ccggacggtg
     1081 cgccgaaagt accggcaatt tgaaagcgac ttcaccggtg tcgatgcgga aattctaacg
     1141 tcccaaatcc ccggtggcat gttgtccaac ctcgctgcgc aactggcgga gcaaaacgcc
     1201 ctcgaccgga tgaaggaagt gatggacgaa atcccccgcg tccggaagga catgggctat
     1261 cccccgctcg tcacgcctac cagccaaatc gtcggcacgc aagccacact caacgtactg
     1321 accggcgaac agggcgagcg ctacaaagtc atcaccacgg aaaccaagaa ctacttcctg
     1381 ggcctctacg gccgtgcacc aggccctctt gataaagaga ttatggcccg agcgatcggc
     1441 gacgaagaac cggtaaaagg ccggccggcg gaccgcctgg aagcagaatt cgaaaaactc
     1501 aagaaggaca tgccggaatc cgccacgacc ctggaagacc agttgtcctt tgccctgttt
     1561 ccggccattg cccgggattt cttcgaggca cgcgagcaag gcgccctgca gccggagccg
     1621 ctcgagccga ccgacaccaa aggcccggcc gtggcgcacg acctccatct ggctccggcc
     1681 gaattcaaca ttacggtgca tggcgagaat taccatgtcg tggtctcagg ctccggccgc
     1741 accaccgacg gccgcaagcc ctattacatc cgcgtcaatg accgactgca ggaagtctcc
     1801 cttgaaccgt tgcaggaagt gttggccggc gtgccggaat ctcccgaggc cagcagcacg
     1861 tcgaagccga aacgtccccg accgacgaaa cccggtgatg tggccccgcc catgccgggc
     1921 cgtgtggtga aggtgttggt gacggatggc gcccaggtga aaacgggcga tcctctcttg
     1981 atcatcgagg ccatgaagat ggaaagccag gttccggccc ccatggacgg gcgggttgcc
     2041 gccatccttg ttgctgaggg agacaacgtc aaaatcgacg aaaccgtcat tcaattggag
     2101 tagccggtgc cgcgcccggc ctctctcgtc cggtttgttg ccccagcgcc ccatcacctg
     2161 gccacctggt ttcttgcgag tctcctcatg agcggttgcg cggcacggcc ggatgtccct
     2221 ccctggttcg acgcgatcca gcgattgcct gttcatacca tttccgtcac cggccaccgg
     2281 atcgcctacc ttgatgaagg cgaggggccg acactgattc tgctccatgg ctacggcgga
     2341 tccatgtggc agtgggaata ccagcagccc ttgaccaagc acttccgtgt gattacgccg
     2401 gatctcctcg gctccggcct ttccgagaaa cccgacatcg aataccgtcc ctccgcactg
     2461 attgaatcca tacgaggact catggatggg ctgggcctgc agacagccac cgtcatcggc
     2521 aattcgatgg gcggcggcgt ggccattggc atggccctga catatccgga gcgcgttgac
     2581 cgtctggtct tgatcgatac cctgccggac catgttcggg aacgactcgt gagcccgctc
     2641 atgcagcggg ccatcaacac gcacgtgccc gcctggatgg cccggttctg ggcatcgctg
     2701 ttcggaagcc ggacgatgga agccgtgctc aaggaagttg tctacgatca cagcctcatc
     2761 accccgctcg tgctggagcg atcaaaccga aaccgccagc gggaagacat gatcggcccc
     2821 ttactctcgt tgcgcgacag cctgccgctg tgggagcagc tgtatgcgcc gcgactcaca
     2881 gcaattcgcc agtccacgtt gatcctgtgg ggcgagcatg atcgcctctt ccctccgcaa
     2941 gtcggccaag atctccaggc gatgattccg cactcacggt tgattgtgat tcccgaggcc
     3001 ggccacattc ctcaatggga gcggccacag gcggtcaacc ggcacatcat ggagttcctt
     3061 cagccttgac agtcttccca gcgggccata cagtgacaat ggagagcgta cgtccctttt
     3121 ttcatggaga ggtcttatga ggtcgatgag tgcccctgtc ctgtctggcg ttctggttcc
     3181 acgtcttctt gcaggcctgt tgtgtctcgg cctgagcgcg tgcggaacct tgccgggcac
     3241 tggcaagggg gggttctcat acaaagacat gccgatcgcc gacggaagcg cagtggccgg
     3301 agaaggcaac aatatcctgt ttcagggcaa gccgctgatg ctcaccggta tgggcatcaa
     3361 ggtcggcgac aaattgcgcg atgtgaaatt ggcgcaaatc gacctctcga tgattcccat
     3421 caatgacacc aagggcaaag gcaaggtccg catcatcagc atcgtcccgt ctctcgatac
     3481 gaaagtctgc gaacagcaaa cgcactattt gagcgagaag aacatgggcc tcgatcgcat
     3541 ggtcgaactc atcacgatca gcatcgacac gccgttcgcg caaaaacgat tcgcagaaga
     3601 agccaagatc agcaatgtca cctttctgtc cgattttcgc gccgccgatt tcggcaaggc
     3661 ccatgggctt ctgctgaaag accttcacct cctgagtcgt gccctgctgg ttgtcgacaa
     3721 agacaacaag gtgcgctatc tgcagatcac cccggaatta gctcaactgc cggacctaga
     3781 ggatgccttc cggttcgcgc gtaagctggt gacggccagc tgactcttgt cttacggccg
     3841 ttgaggacta tccatggccc attacgatct gctggtgatc ggaaccggcc cggcaggaca
     3901 aaaggcggcg attcaggccg ccaagctcgg caaaaaagcc ggcatcgtcg agcgcacacg
     3961 ggttgtcggc ggcgtctgca ccaacaccgg gaccatcccc agcaaatcgc tccgagaagc
     4021 ggccctctat ctctccggtt tccatcaacg tagtctgtat ggcgccagtt accgcgtcaa
     4081 acaggacatc acgatggcgg atctgacctt ccgttccaat cacgtcatca accgtgaaat
     4141 cgaaatcatt cagaaccaga tgacgcggaa caatatcgac ctgtggtacg gcaccgcgtc
     4201 cttcgtcgat ccgcatcgcg tacgcgttga gcgggctgat gatcacgtgg aacacacggc
     4261 ggatttcatc gtcatcgcct gcggcacggt tcccgcccgt cccgcccaca ttcccttcga
     4321 cgatcagacg atcatcgata ccgacggcct cctgaagctg aaggacctcc ccaagtccat
     4381 caccatcatc ggcggcggag tgatcggcgc agaatacgct tccatcctgg ccacgatggg
     4441 cctccacgtc acgctcatcg agcgccggcc gcgcctgctg gaatttgtcg accaggaaac
     4501 cattgaagcg ctgcagtacc atatgcgcag tatcggagtc gtccttcgat tcaacgaaga
     4561 ggtcgtgtct gtcgagccga tctccaaaga actagtcatc gtccgcttaa agagcgggaa
     4621 agaaatcgcc gcctccaccg tgctgtattc ggtcggccgt accggcgcca gcgcgacgct
     4681 taatctcgag gccatcggac tcgcagcaga tgagcgcggg cgcctcacgg tcaacgagca
     4741 ttatcaaaca tccatccctc atatttatgc ggccggtgac atcatcggat ttcccgcgct
     4801 cgcctccacg tccatgcagc aaggccgtca cgccgcttgc catgcgtttg gcgtgcccta
     4861 ccagacgcaa tcggacctga tgccctatgg catctattcg atacccgaga tttcgatggt
     4921 cggccgcaac gaagacgacc tgaccaaaca cggagtcccc tatgccgtcg gcatcgcccg
     4981 ctaccgcgaa atcgcccgcg gccagatcat cggtgatgag atcggcatgt tgaagctgct
     5041 ctttcacaat aagacccgtc aattgctcgg cgtgcatgca atcggcgacg gtgcgacgga
     5101 actgattcac atcgggcaga cggtgatggc ctttcaagga cagatcgatt acttcatcga
     5161 tgcggtgttc aattacccca ccctcgcgga atgttatcgc gtcgccgcgc tggatggcat
     5221 caatcaactc ccgagaccct ggacaccgcg agcctgacag gcccctgtct cgcgagccgc
     5281 aatagtcctc aatgccccat gtcaggcatt gaactgagga gtgcccatcg agaattcaat
     5341 agtctccgta agagcaagga ggacccggat gtcgttttca aatcatcttc gtaagctggc
     5401 ccaacccgtg tgggatgcac aactgacgca ccccttcgtc ctcgcattag gcaagggcac
     5461 cttgtcggaa agaaaattcc gctattacat cctgcaggac gcccggtttc tcgcagacct
     5521 tgctcgggtc tttgccgccg ggtccttacg agctccggac tccgaatcgg cgcttcgctt
     5581 cgccaagctg gcagaagaaa ccatcaccgt cgaacgcagt ctgcatgaaa actacggcaa
     5641 acgctggaac ctttcgccgc aagagatgct gaacgagccg atggcgccga ccaactatgc
     5701 ctacacccgc cacatgctgg ccgtggccca agcgggtacg gcgacggaaa tcgccgtcgt
     5761 ggcgctcccc tgcgcgtgga tttattgtgt cgtcggcaag cattttctca agaacggagc
     5821 gccgccggca aaacacccgt accgcgattg gctgatgctc tatgcgtcac cggagttcga
     5881 ggcagttcag gaatggatgc gcgccagggt cgatacctgg gcgcgaacag ccgggcatga
     5941 agaaaaaaaa cggatggagc aggcgtttct gatcagttcg aagtatgagt ggatgttttg
     6001 ggaaatggcc tggaatgagg agaagtggcc ggtgtgaggg ggcgggcagc gatggcggca
     6061 ctctacacgc cggaaacaaa gccgcagcag cgttcggcag tgaggtgatc ctttccgcct
     6121 gaacttgctc tgttcgtgct cgcacgaaca gagcttcgaa caacaggcgt cacctgcaac
     6181 gcaggacggt tcaccccacc gtcgaacgag gtcctgctga cgctttgagg gcgttccgag
     6241 tgtcgccatc gctgcccgcc agtcacacgt ccactgaaag gtagctgaaa taacaatact
     6301 tccagcatag cggaacccta atttatcggc tttctactgt gtacttacgc gacaggagct
     6361 tgaaggaata actagactat gccacctcat gaggaaggaa aaagaaaaat cagtttcccc
     6421 aattcgaacg taagcggggg aaatatcgtg gtggttctga tcgaaaatgg gaagagccca
     6481 gatcaaccct acaatgcccg tcttggattc tggttgcctc cagattctcc ctatggcaac
     6541 tttcaactga aacttagaaa gctgtgcgag agactcgacg aagctaatcg tagattgatc
     6601 gacactttcg attattggaa tcaggcaatg gagcagaaaa cttcagtcaa cttgttccaa
     6661 cgtcacgagt atgcaatcga acaggctgtc tatctcatga gacgagtggc tgacgaattg
     6721 atagcaatga tctggtgtct acatgagtgg gaaaaaacgg gggactatcc gcgccaaatt
     6781 aggattgatt gtatcggtgc cgcgcttgaa tctacgcaac aaaacctttc aaaagacctt
     6841 aaagtgcttt ttgaccctca caatccagtc atgaaagctc tcaacgaaat ttcaaatgca
     6901 ttcaaacact ccttcgttca ctcagattta acattgattg gaagggacga gccctgcgtt
     6961 tatgctctcg ggttagccca taataaactt tcttctgaaa tgaaattcca taatttctcg
     7021 gtgtcttggt tagtacgcga attcagtgcc ttttataaat ccgggatgca atggctcgca
     7081 tcattttccg aaagacatcg aaaatgaatt cagccttcaa tattcactgt tccctatgca
     7141 cgcgcatagg attccaccca aacggcagca ttctcacttc tcaataaagg ggtcgccccc
     7201 cactcggaat atgtccctaa gagaatcgcc gctccgattg atctctcgtt caggcacgac
     7261 actgcaggct acacagcccc tctctgaccg ttagcatggg acacaccgta cagtcctacc
     7321 actcgttcct gtcctattta ggatagaggg ctgacacgcg tagtgtctga tttatccgaa
     7381 caggcctcta cctggcccta accggtcttg cgatattcct gggactggtt aagcggttcg
     7441 acatcccaat cgggttcttc ttcacccctc attggaatac tctcctgagt cacggacgcc
     7501 cagatggcga tggcacaaaa caggatcgtc aatgcgatga ggaagtaaac aggcataatg
     7561 acctcctcta ttcctggttg tcctactgtc acggtaatcc tctcatccgc tataggctac
     7621 agggtaataa cctaggttat ggacggattt tccctgtagt ggaaaggagg atagctccac
     7681 ctgattcacc cagaaccgtc gctcgatccg cctgttccgg gatgttcagt agccgacgct
     7741 ttcagattta ttccacgttt gtggtaggct ggccgcatcg tctgaaacgc gactctttaa
     7801 tcccatatga ctgacgccgc atccaccccg accgttctga gctcacgcga gatccaactc
     7861 catctgctgg ggctcgtgct gctgctcggt gtgctagggc tgtggtactg gttttcgacc
     7921 ggagcgcggg ttgtcccgac cctcatcgag ctactcaagg acgatgatcc gtcgacacga
     7981 attgtcgcgg ccgaacaatt agggcaagtc ggaccggcag cgcgatccgc cgttccgcaa
     8041 ctcctgagcc aggccacgca ggacggcagt cagcatgcca acaccaccgc cgccaccgcg
     8101 ctgaagtgga tcgatttgac ggcggctcgc cgggtgatga cccatttcgt cccgagacta
     8161 caagatcccg atgtgcaact acgccgcaca gcctgcgcgg tgatcggcag tctggggccg
     8221 attgccaaac cggcggtacc ggccctgctg gccgtcacgc atgatgccga tgcactcata
     8281 cgccgcaatg cgctcatcgc cctggcgagc atcggcattc cgtctctccc gatcgcggcc
     8341 gccctcgtgg acggcctccg cgattcttcg tcgctggtgc gccagacggc ggtcgcccaa
     8401 tttgcgttta cggttccccc ttcgccggat gctgtggcgg ttctgacgcc aatgctgaac
     8461 gatccggata aaggcatcgc caccctcgca cgtacggcct tggacaaacc gagggccggg
     8521 gatgcagccc atatcgaatc gttgggcatg atgctcggac attcctctgc acgggactat
     8581 gcgttgcacc aactggcaca attgggcccg gccgcccatg atgccttgcc cccggtcctt
     8641 gcgctgctgg aagatgaaca gcctctgatt cgctatctgg ccgccgaaag catcggggga
     8701 atgggagctg ttgccaaaca agcgctgccg gccttgcaac gacaccagga ttccgacctc
     8761 gtagttcgcg ccgctatcgc cgaggccgtg agcgcgatcg aagccggggc ggtcgtcaac
     8821 gccgaaccgt tgagcaagga actgccacgt tgaccgatat cccgcggcaa acatcgcctc
     8881 gcctccggat gatggcgcga ttgggggtct ggatcagcct cctcggcgga ttgcttcttc
     8941 cctggatcat catgctgacc gtggagatcc tggttcgcga catcccctat ccacgggcct
     9001 ggaggtcgtt caccttgcac ctctttgcgc ccggctataa ttttttcttc gtgggggtgc
     9061 tgacggcgct gccgttcgtg atcctggccc tgatcatcct gttccacctg gggaccgccc
     9121 cggcgaacaa cgccttgatt gcctaccggc gggcactcgg cctcttctca gcgggactga
     9181 gcatggccct catcgccgcc tgggtccatg tcgacgtgct catccatccc gatgcgcagg
     9241 gagcgctcgc ctatttctac ttgccgattc ttttgctgat gatgctgccg ttcggatacg
     9301 ggctgggacg cgcattggcc aaaatctttc tgccccgact gtcggggtga cgcgcacggg
     9361 ctggacagca tcgacagcgt gagtataatg tgggccatgc gtcgcgcgat cgtacacggg
     9421 ggccgatgat tcgtctctta ccctgcctca tcctgcttgt cctctgccat tccacctctc
     9481 tggctgcctc gaaatatgag gacagtgtca aagaactcgc cgaaggggtg gcggctgagg
     9541 ccatcaaaat gaaaaagtcg cggctcgcac tgctggactt tgtggacagc aagggagacg
     9601 tgacacccat cgggcaattc ctggccgaag agctggcaac tcatctgctt gtggccggag
     9661 agctcaaagt ggtcgatcgc aagttgctga tcgcgaccat ggaaaagcac cagctgaccc
     9721 acctcgatac gtcacaagcc aaggccgcca agaaagtcgc caaagccctg cgcgcagacc
     9781 tctacgtcac cggcgcgtat ctggagattc ccgaaggcat tcaggtcacc gccaagctga
     9841 ttgggcccta tacggtctac cccgtcggcg cctcacggac catcgtaccg aaatccggcc
     9901 ccttggcagc cctgctgaaa caggcagccg ctccggctcc ggccacggcg gccgtggtaa
     9961 cggatagtgc gccggtcctc agcgctcacg aaaatgactt gtacaaattc acgattctca
    10021 gcgtgacacg gcaggacgac ctcatcggtc tcgatctcgt cttcaccaac cgtacggcgc
    10081 acccggtcaa gatcggctgc cagcttctcg acacctatct tgtggacgag catggcgagc
    10141 agtggaaaca gaatgtcgcg cagaatcgtg aaggcctctg tacgcgcggg atggagctga
    10201 accccaagcg gcagcagcgt gcgagcctct cgttcctggc caccaataag ccgccgacag
    10261 gcccgatcac gttgcactac catgaaacgt cacccagggc tgatcggatc atcaccctgg
    10321 acggattgaa aattgcctcc gcgccggtcg ttgagccagg cgccccggcc gcgcaggaat
    10381 caacgccccc cacaccgtag gtccacgtct cattacttgc acaggaactt acccatgatt
    10441 aaacaagtct tgtcgatagc tggttctgat tcaggcggtg gcgcaggcat tcaggcagat
    10501 ctgaa
//
LOCUS       DAOQDS010000098        10472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_3175_length_10472_cov_22.064990, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000098 DAOQDS010000000
VERSION     DAOQDS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3175_length_10472_cov_22.064990"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PK589_17000"
     CDS             <1..479
                     /locus_tag="PK589_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNJ21364.1"
                     /translation="SFLERQLDANILTTNLDAFVGWARKSSLWPMTFGLACCAIEMIA
                     SVSSRYDIDRFGAGVFRASPRQSDLMIVAGTVSRKMAPVIRRIYDQMAEPRYVISMGS
                     CATSGNHYNSYAVVQGVDQIVPVDVYIAGCPPRPEALIDGLLKLQQKIQREKIFVK"
     gene            495..2249
                     /gene="nuoD"
                     /locus_tag="PK589_17005"
     CDS             495..2249
                     /gene="nuoD"
                     /locus_tag="PK589_17005"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNJ21365.1"
                     /translation="MQPLLERLMTTFPDAVRGVEVDAARNEVTARVSAARIVDVARWL
                     HDTPEAAFDHITDICSVDYPNDTERFEVIYQLLSLAHRRRIRLKVRITEDHPEVASVT
                     GIWKGADFMEREVYDLMGITFVGHPDLRRILLPEDYGEGHPLRKDFPAEGRGWRSEFP
                     FIPRLDEAPEELSAEGEIPESEKQVYRATEQPSSTRRREELLLNMGPQHPSTHGVLRV
                     VLELDGERIVKATPDLGYLHRGVEKLAEGLHYMQVIPHTDRLDYVCAMTNNYAYVRAV
                     EKLLDITVPERAEYIRTVVAEMQRIIGHLFWLGTQALDIGAMTVFFWTFREREILLDM
                     FEKLCGARLTLNYYRIGGVDSDFTPDLVTRLKAFLHTFPDKVNEYNQLLMSNRIWVGR
                     TKDIAVISAEDAINFGLTGPTLRGSGVDYDIRKYEPYGAYDKVEWEVPVGKRGDTYDR
                     YWVRMEEMRQSARIIAQCLDQMPEGPIMADVPHVIPPPKAKVMRDMESLIHHFIIFTQ
                     GFKPPKGETYCGTEVPKGELGFFIVSDGSPRPYRLKIRAPSFIHMGAFDHMARGYLIS
                     DIITIFGTYDIVMGECDR"
     gene            2370..2906
                     /gene="nuoE"
                     /locus_tag="PK589_17010"
     CDS             2370..2906
                     /gene="nuoE"
                     /locus_tag="PK589_17010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNJ21366.1"
                     /translation="MLKEKYQAEIDEILSRYPVKRSALLPLLYLAQREEGYVTEAAMQ
                     EIAGILKLTPPQVYETATFYTMLNLKPVGKFHLQVCKSLMCALVGSDTVIGWIGTKLG
                     IKPGETTQDKLFTLSIVECLAACGTGPMMQVNDDYYERLTEEKLDRILADLRQTGTCS
                     LKTGPFMWPEPAAAKSGA"
     gene            2973..4280
                     /gene="nuoF"
                     /locus_tag="PK589_17015"
     CDS             2973..4280
                     /gene="nuoF"
                     /locus_tag="PK589_17015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNJ21367.1"
                     /translation="MKTYEPILLKNMIQPGYAGSLPEYERAGGYQALRKVLGKVTPAE
                     VTAVVMKSGLRGRGGAGFPTGVKWGFLPKDYQGPRYLCCNADESEPGTFKDRQLIERD
                     PHQLLEGMLLACYAIGAASSYIYIRGEFVLGAKILEAAIKEARAAGYVGKNILGTGVS
                     IDIWVHRGAGAYICGEETALLESLEGKRGLPRVKPPFPATHGLYNKPTVVNNVETLAN
                     LPHIMNRGAEWFASIGSPPKSTGTRVFCVSGHVARPGNYEVPMGITFRELIYEQAGGM
                     RSDKKLKAFIPGGASAPFLTPDHLDVKLDFESVALAGSMLGSGGVTVMEEGTDMVWAA
                     LRLMEFFYHESCGKCSPCREGSSWLVQIMRRIVNKRGRMEDLETLTDLCKNIAGRTVC
                     AFGDAEVSPILSTLKHWRHEYVTMIHAAEAANLIRPEPAGAKH"
     gene            4317..6986
                     /gene="nuoG"
                     /locus_tag="PK589_17020"
     CDS             4317..6986
                     /gene="nuoG"
                     /locus_tag="PK589_17020"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNJ21368.1"
                     /translation="MPDQKPETVRLTIDGTTVAVPKGTLVIEAARRVGVMIPHFCYHP
                     KLKPDANCRMCLVEVEKMPKLQTACSTPVAEGMAVRTATTTVDDAHKSVLEFILANHP
                     LDCPVCDQGGKCDLQDFSHQYTPTTSRFTETKRIFQKEYFSPLIETQMNRCVQCLRCV
                     RYCDEIMDVKALAPVGRGTMTEIKHFGPHELDCEFCGGCIQICPVGAITSRLSMYEYR
                     PWMLKRADTICTFCGDGCRITVQTKGNELIEVNSSHGAGRNNGDLCARGFFGFHASTH
                     ADRLTHPLIRRDGVLVQATWEDALEYVAEQALRVKLANGPDAFGGLISSRCTNEDLYV
                     FQKFMRQVIGTNRIDSSARYGHLSGVRALQRVQGTHRWTIAFEDIVAANALLLVGTNI
                     TETSPITGLKVKEAVKKRQASLVTIESLEPAVDTLSNIANLATQHFSAHPAQIGNNVL
                     GLIKAVIDGNLVDAGLAEQSPAFVQRLSAATQQISWDTLETATGHTRAQWAETAKLLA
                     AAKRVVILVGNGVLRHPGGEGTTTNLLDLLILLGKLNQPGCGLGPLAEENNDQGAVEM
                     GAVPEFLPGPAPLNDQAARDRLASLWREELPRTAGISLTEMLAAANSGFLKAMFVVGE
                     NPIGTLPTGAKAKEALGKLELLVCQELFLTETAAMAHVVLPACSYMEKDGTFTNSEGH
                     VQAVRQAINPIGDSRPDWEMLSAISVLMGSPLEYGDAREILKEIRSVIPGYGLLGPTP
                     TSPKVDQPILSRYLAEDASTDMASRYALRQAPKSSETSLTLLFTQTLFHSGKLSTHSK
                     GLLQLQQEGFLSLNPVDAAKVGVADGEKVTVSNARGSITTIAKIRERVPAGLVWFPEH
                     FDGEAKQLAEWTIDPRTQIPYFKLAHVSLAKVS"
     gene            7011..8078
                     /gene="nuoH"
                     /locus_tag="PK589_17025"
     CDS             7011..8078
                     /gene="nuoH"
                     /locus_tag="PK589_17025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNJ21369.1"
                     /translation="MEIGLRLAVSLAQIAAVMGVVMLTVMILTLAERKVLGWMQDRMG
                     PMEVGPYGVLQPIADGLKLFFKEDIIPAGANKFLFTLAPILALVPAMIGFAVIPFGPT
                     MTIELFGMQVKPFVISDINIGILYILAFASIGAYGIILGGWSSNSKYSLLGGLRSAAQ
                     VISYELNVGLAIVGVILLSGSLSLVKITEAQAGGFWNWFIIAMPFPQIFAFVVYVISS
                     VAETNRVPFDLPEAESELVAGFFTEYSGMRFAFFFIAEYANMILVSCVAAALFLGGWN
                     APYPGTILGHLGLDALAWIENVVWFAAKVYFFLFLFFWLRATLPRLRYDQLMRFGWKV
                     MLPIALGNIVLTAIAAYFFPR"
     gene            8088..8663
                     /gene="nuoI"
                     /locus_tag="PK589_17030"
     CDS             8088..8663
                     /gene="nuoI"
                     /locus_tag="PK589_17030"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNJ21370.1"
                     /translation="MNVAITAQPKRKPSFSDWLKTLTFYELLVGMKATLKHLLNYKPI
                     TLQYPHEKRLLPENYRGMLALLRYDDGTEKCVGCDLCEAACPSRVIRVVSGEVPGEPT
                     KRYSKEYYMDMTRCLFCGLCVDACPVDALGMTREFEWAVYDKRQLHLNKQQLLAIGDR
                     AYPVREKRLELQHPNVAFFNVTFKHLPQKEN"
     gene            8791..9306
                     /locus_tag="PK589_17035"
     CDS             8791..9306
                     /locus_tag="PK589_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNJ21371.1"
                     /translation="MDHIFFFYFAAVIATTSVLVVALRNPVYSALALLIMFFHVAGLY
                     VTLHAEFLAAVQIVVYAGAILVLYLFVVMLLSIKSEERYHNQLPVAGLLGVMLCTEII
                     LLLIQSRTAASTQAVPADPVTGSGNTEMVGEALYSTYLFPFEVASLILLVAMIGAIIL
                     AKKDINEQVQQ"
     gene            9318..9623
                     /gene="nuoK"
                     /locus_tag="PK589_17040"
     CDS             9318..9623
                     /gene="nuoK"
                     /locus_tag="PK589_17040"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNJ21372.1"
                     /translation="MTVPLSYYLVLSGFVFLTGVVGVLIRRNIIVILLSVELMLNATN
                     INFVAFSEYFHNVAGQVFVFFALTVAAAEVAVGLAIIIALHRTNSSIYIDDLNLLKR"
     gene            9640..>10472
                     /locus_tag="PK589_17045"
     CDS             9640..>10472
                     /locus_tag="PK589_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNJ21373.1"
                     /translation="MMYALIPLLPLFSFLIVGIGEQWIKDRAHLVAVPAMVGSFLLSL
                     MALHDVATGQAITVNLYTWLTSGNLDIHIGISIDRLTAVMLILVTTVSTLVHIYTIGY
                     MHGEPGYARFFAYIALFTFSMLMLVMADNLLQLFVFWEAVGLCSYLLIGHWYERASAC
                     AAATKAFLVNRVGDFGFILGLFLVWYSFGSLDYATVFAQAGQLASKTTNLLGPFGGTW
                     DVSVMTMICLLLFTGAVGKSAQVPLHVWLPDAMEGPTPISALIHAATMVTAGVFMVAR
                     LA"
BASE COUNT         2118 a         3148 c         3034 g         2172 t
ORIGIN      
        1 tgagcttttt agagcggcag ctggatgcaa atattctgac gacgaatctc gatgccttcg
       61 tcggctgggc gcgcaaatcg tccttgtggc cgatgacgtt cgggttggcc tgttgcgcca
      121 tcgaaatgat cgccagcgtg tcttcacgct atgacatcga tcggttcggc gccggcgtgt
      181 tccgggcctc gccccgccaa tcggatctca tgatcgtggc cggcacggta tcccggaaga
      241 tggctcccgt cattcgtcgc atttacgacc agatggccga gccgcgctac gtcatttcca
      301 tggggtcctg cgcgacctcc ggcaaccatt acaacagtta tgccgtcgta cagggcgtcg
      361 accagatcgt gccggtcgat gtgtatattg ccggctgccc tcctcgaccg gaggcgttga
      421 tcgacgggct cttaaagttg cagcaaaaaa ttcagcgcga aaagattttc gtaaagtaac
      481 cggctccttt catcatgcaa ccgctgctcg aacgactcat gaccaccttt cccgacgccg
      541 tccgcggcgt ggaggtggat gccgctcgaa acgaagtgac cgcgcgtgtg tccgcggcgc
      601 ggatcgtcga cgtcgcgcgc tggctgcacg acacacccga agccgcgttc gatcacatca
      661 ccgatatttg ctcggtagac tatccgaacg atacggaacg gtttgaagtc atttaccagt
      721 tgttatcact cgcacatcgg cggcgcatca gactcaaagt gcgcattacc gaagaccatc
      781 cggaagtcgc ctccgtgacc ggcatttgga agggcgcgga tttcatggag cgtgaagtct
      841 acgacctgat ggggattacc ttcgtcgggc acccggacct caggcgcatt ttgctgccgg
      901 aagactatgg agaaggccac cctttgcgga aagattttcc cgcggaaggg cgcggctggc
      961 ggagcgaatt ccccttcatc ccacggttgg acgaagcccc cgaagaactg tcggcagagg
     1021 gagaaatccc cgaatcggaa aaacaagtct atcgggcaac cgaacagccg agcagcacac
     1081 ggcggcggga agaactcctg ctcaacatgg gcccccagca tcccagcaca cacggtgtgc
     1141 tacgggtggt gttggaactg gatggcgagc ggatcgtcaa agccactccc gatctcggct
     1201 atctccatcg cggcgtcgaa aagctggcgg aaggcctcca ctacatgcag gtcatccccc
     1261 acaccgaccg gctcgattac gtctgcgcga tgacgaataa ctacgcctat gtgcgcgccg
     1321 tggaaaagct gctcgacatc acggtaccgg aacgggcgga gtatatccgc accgtcgtcg
     1381 ccgagatgca gcgcatcatc ggccatctgt tctggctcgg cacccaggcg ctcgacatcg
     1441 gcgccatgac cgtcttcttt tggacgtttc gcgagcgcga aatccttctg gacatgttcg
     1501 agaagttgtg cggcgcgcgt ctgacgctga actattaccg catcggcgga gtcgacagcg
     1561 atttcacgcc cgatctggtc acgcgcctga aggccttcct gcacacgttt ccggacaagg
     1621 tcaatgaata caatcagctg ctgatgtcca accgcatctg ggtcgggcgc acgaaagaca
     1681 tcgcggtgat ctcagccgaa gacgccatca atttcggcct caccggcccg acactgcgcg
     1741 ggtccggcgt cgactacgac atccgcaagt atgagcccta cggcgcctac gacaaggtcg
     1801 aatgggaagt gcccgtcggc aagcggggcg atacctacga ccgctactgg gtccgcatgg
     1861 aggaaatgcg gcagagtgcc cggatcatcg cccagtgtct ggatcagatg ccggaaggcc
     1921 cgatcatggc ggatgtgccg catgtgatcc ctccgcccaa ggcgaaggtc atgcgcgaca
     1981 tggagagcct gattcaccac tttattattt tcacccaggg tttcaagccg ccgaaaggcg
     2041 aaacctactg cggcactgaa gtcccgaaag gcgagctcgg gtttttcatc gtcagcgacg
     2101 gcagcccccg cccctatcgg ctcaagattc gcgccccctc cttcattcac atgggcgcat
     2161 tcgaccatat ggcgcgcgga tatctgattt cagacatcat cacgatcttc ggcacctacg
     2221 acatcgtgat gggagaatgt gaccgatgag gcgcatgagc caggtgtccg gagtgctcgc
     2281 ggaatgcgcg gtctgagcag acccccgttg gacgcaccga gtacccccga cgtggaactc
     2341 accgtaactg gcagcgtgga gtgagagaga tgctgaaaga aaaatatcag gcggaaatcg
     2401 acgagatcct gtctcgttat ccggtgaagc gatccgcgct cttgcccctg ctctacttgg
     2461 cgcagcgcga agagggttat gtcaccgagg ccgccatgca ggaaattgcg ggcatcttga
     2521 agctgacccc gccgcaggtg tatgagacgg ccacgtttta cacgatgctg aacctgaagc
     2581 cggtgggaaa gttccacctg caggtctgca aatcgttgat gtgcgccctg gtcggctcag
     2641 ataccgtcat cgggtggatc ggcaccaagc tcggcattaa gccgggcgaa acgacccagg
     2701 acaaactgtt cacgctcagt atcgtcgaat gcctggccgc gtgcgggacc ggcccgatga
     2761 tgcaagtgaa tgacgattat tacgagcgac tgacggaaga gaaattagac cgcattctgg
     2821 cggatttgcg gcagacaggc acctgctcgc tgaaaaccgg gccgttcatg tggccggaac
     2881 cggccgcggc aaagtcgggg gcctaacccg ccctcgtttg cgattcgctc aaaggagact
     2941 gctcgcgtgt cacgttccat gagatggcct gtatgaaaac gtacgaaccg attctgctca
     3001 agaacatgat tcagccgggg tatgccggct cgctgccgga atatgagcgc gcgggcggat
     3061 accaggcgtt gcggaaggtg ctggggaaag tcacccccgc cgaggtgacc gccgtcgtca
     3121 tgaagtccgg attgcgcgga cgcggcggag ccggattccc aaccggcgtg aagtggggat
     3181 ttttgcccaa ggattatcag gggccccgtt acctctgttg caatgccgac gagagcgagc
     3241 cggggacgtt caaagatcgc cagctgattg agcgcgatcc gcatcaattg ctcgaaggca
     3301 tgttgttggc ctgctacgcc atcggcgcag ccagttccta catctatatt cgcggtgagt
     3361 ttgtcctggg agccaagatt ctcgaagccg ccatcaagga agcgcgcgcc gcaggctatg
     3421 tcggcaagaa tatcctgggc accggtgtct cgatcgacat ctgggtccat cgcggagccg
     3481 gcgcctatat ttgcggtgaa gagacggccc tgctggaatc gttggaaggc aaacgcggtc
     3541 ttcctcgcgt caagccgcca ttcccggcca cgcacggtct ctacaataaa ccgacggtcg
     3601 tgaacaacgt cgaaaccctc gccaatctgc cgcatatcat gaatcgcggc gccgaatggt
     3661 ttgcctcgat cgggtctccg ccgaaaagca ccggcacacg agtcttttgc gtgagcggtc
     3721 acgtggcgcg acccggcaac tacgaagtgc cgatgggcat tacgtttcgt gagttgatct
     3781 atgaacaggc cggcggcatg cggtcggaca agaaactcaa ggcctttatt ccaggaggcg
     3841 cttccgcccc ctttctcacg cccgatcacc tggacgtcaa gctggatttc gaatcggtgg
     3901 cgctggccgg atcgatgctg gggtcgggcg gcgtgacggt catggaagag ggcaccgata
     3961 tggtctgggc tgccctgcgc ctgatggaat ttttctatca cgagtcctgc ggcaagtgca
     4021 gtccctgccg ggaaggcagt tcctggctcg ttcagatcat gcgccggatc gtcaacaaac
     4081 gcgggcggat ggaagacctt gaaaccctga ccgacctgtg caaaaacatc gccggacgga
     4141 cggtctgcgc attcggtgac gccgaggtgt ctccgatttt gagcacactc aaacattggc
     4201 ggcacgagta cgtcaccatg attcatgcgg ctgaggccgc caacttgatc cggccggaac
     4261 cggcaggagc gaaacattga cgtctctcgt gtcacgttgc acgaataacg gccactatgc
     4321 ctgatcaaaa accagaaacc gttcggctga ccatcgacgg caccaccgtc gccgttccca
     4381 aaggcacgct cgtgattgaa gccgcgcgtc gcgtgggggt gatgattccg catttctgct
     4441 atcaccccaa gttgaagccg gatgcgaact gccgcatgtg cctggtcgaa gtcgaaaaga
     4501 tgccgaagct gcaaactgcc tgcagcacgc cggtggccga aggcatggcc gtgcgaaccg
     4561 ccaccaccac ggtggacgat gcgcacaagt cggtgctgga attcattctg gcgaaccatc
     4621 cgctcgattg tccggtctgc gaccagggcg gcaagtgcga ccttcaagac ttctcgcatc
     4681 aatacacacc gacgaccagc cggttcacgg aaaccaagcg catctttcag aaagagtact
     4741 tcagcccgct catcgaaacg cagatgaacc gttgcgtgca gtgtttgcgc tgcgtccgct
     4801 attgcgacga aatcatggac gtcaaggccc tggcgcccgt cggccgcggg acgatgacgg
     4861 aaatcaaaca tttcggcccg catgaactcg actgcgaatt ctgcggcggc tgcatccaga
     4921 tctgcccggt cggcgccatc acgagccggc tgtccatgta tgaatatcgg ccctggatgc
     4981 tcaagcgggc cgatacgatc tgcaccttct gcggcgacgg ctgccgcatc acggtccaga
     5041 ccaaaggaaa cgaactcatt gaagtgaatt cgtcgcacgg cgccggacgg aacaatggcg
     5101 acctctgcgc ccgcggcttc ttcgggttcc atgcaagcac ccatgccgat cgcctgaccc
     5161 atcccttgat ccgacgcgac ggcgtcttgg tgcaagccac gtgggaagac gcattggaat
     5221 atgtcgccga acaggccctc cgggtgaagc tggccaacgg cccggatgcg ttcggagggc
     5281 tcatcagctc ccgctgcacc aacgaagacc tttacgtatt ccagaagttc atgcgtcagg
     5341 tgatcggcac caatcgaatc gatagcagcg cgcggtatgg ccatctgagc ggggtgcgag
     5401 cactacaacg ggtgcaaggg acccaccgct ggaccatcgc gttcgaagat attgtggccg
     5461 ccaacgcctt actgctggtc ggcaccaaca tcacggagac cagccctatt accggcctca
     5521 aggtcaaaga agccgtgaag aagcgccagg cgagtctggt cacgattgag tcgctggaac
     5581 cggccgtcga cacattgagc aatatcgcga acctggccac gcaacatttc tctgcccatc
     5641 ctgcgcagat cggtaataac gtcttgggcc tcatcaaggc cgtcattgat ggcaacctgg
     5701 tggacgcagg cctggctgag caatcgccgg ccttcgtgca acggctatcc gccgctacgc
     5761 aacagatttc ctgggacaca ctcgaaaccg ccaccggcca tacccgcgca caatgggcgg
     5821 agaccgccaa acttctggcc gcggccaaac gcgtcgtgat cctcgtgggc aacggcgtcc
     5881 tgcgccatcc cggcggagaa ggcacgacca cgaatctcct ggatctcctg atcctgctgg
     5941 gcaaactcaa tcaacctggg tgcggacttg gaccgctggc cgaagaaaac aacgatcaag
     6001 gcgcagtcga aatgggcgcc gtgcctgagt tccttcccgg accggccccg ctcaatgacc
     6061 aagccgcgag ggaccgtctg gcatcgctgt ggcgcgagga gcttccccgc acagccggca
     6121 tctcgttgac cgagatgctt gccgccgcca atagcggctt tctgaaggcc atgtttgtgg
     6181 tgggagagaa tccgattggc accttgccga ccggggcgaa ggcgaaagag gctctcggca
     6241 aactggaact gctggtctgt caggaactct ttctcacgga aacagcagcg atggcccacg
     6301 tagtgcttcc cgcctgctcc tatatggaaa aggacggcac cttcaccaat tccgaggggc
     6361 atgtgcaagc cgttcgtcag gcgatcaatc cgatcggcga cagtcgcccc gattgggaaa
     6421 tgttgtccgc gatttccgtc ttgatgggct cgccactgga atatggcgat gcgcgggaga
     6481 ttctgaaaga aattcgcagc gtcatccctg gttatggcct gctaggaccg acacccacgt
     6541 cacccaaggt ggaccagccc atactgagcc gctatctggc ggaagacgcc tcgaccgaca
     6601 tggcaagccg gtatgctctg cgacaggcgc cgaaatccag cgagacgtcg ttgacgctgc
     6661 tgttcactca gaccctgttc cactcaggca aactgtccac ccattccaag ggactcctgc
     6721 aattgcagca ggaggggttc ttgtctctca atccggtgga tgcggccaaa gtcggggtgg
     6781 cggacggcga aaaagtgacc gtctcgaacg cacgcggctc catcaccacc atcgcgaaga
     6841 ttcgcgagcg tgtgccggcc ggcctggtgt ggtttcctga acatttcgac ggcgaggcca
     6901 aacaactcgc tgaatggacg attgatcccc ggactcaaat cccctatttt aagctcgcgc
     6961 acgtgtctct ggcgaaggtc tcgtaggacg ggacgaggag tattgctgtt atggaaatcg
     7021 gattacgact ggcggtgtcg ttagctcaaa tcgccgcggt aatgggagtg gtgatgctga
     7081 ccgtcatgat tctgaccctc gcagagcgaa aagtgctcgg ctggatgcag gatcgcatgg
     7141 gcccgatgga agtcggcccc tacggcgtgc ttcaaccgat cgccgacgga ttgaaactgt
     7201 tcttcaagga agacatcatt ccggccgggg cgaacaagtt cctctttacc ttggcgccta
     7261 ttctcgccct ggtgcctgcc atgatcgggt tcgctgtcat tccattcggc ccgaccatga
     7321 cgatcgagct gttcggcatg caggtcaagc cgttcgtcat cagcgatatc aacatcggca
     7381 ttctctatat tctggccttt gcctctatcg gcgcctacgg catcattctc ggcggctggt
     7441 cctccaacag caagtattcc ctcctcggcg gactgcgttc cgccgcgcag gtcatcagtt
     7501 acgaattgaa cgtcgggctc gcgatcgtgg gcgtcatcct cctgtccggc tcgctgagcc
     7561 tggtgaagat caccgaggcg caggccggag ggttttggaa ctggttcatc atcgccatgc
     7621 cgttccccca gatttttgct ttcgtcgtct atgtcatttc gtcggtagcg gaaacgaacc
     7681 gtgtgccctt cgaccttccc gaagcggaaa gcgaactggt ggcgggcttc ttcacggaat
     7741 acagtgggat gcgcttcgcg tttttcttca ttgcggaata cgcgaacatg atcctggtgt
     7801 cctgcgtcgc cgcggcgctc tttttgggcg gctggaatgc cccctacccc ggcacgattc
     7861 tcgggcacct cggactggat gccctggcct ggatcgaaaa cgtcgtctgg ttcgccgcaa
     7921 aggtctactt cttcctgttc ctgttcttct ggttgcgggc cacattgccc cgcctgcggt
     7981 acgaccagct catgcggttc ggatggaagg tgatgttgcc catcgccctc ggcaacattg
     8041 tgttgacggc catcgccgcc tatttcttcc cgcggtaacg gagcgagatg aacgtcgcca
     8101 tcacggcaca gcctaagcga aaaccgtcgt tcagcgattg gctgaaaacg ctgaccttct
     8161 atgaactgct cgtgggcatg aaggccacgc tgaagcacct actcaattac aagccgatca
     8221 cgctacagta tccgcatgag aagcgcctcc tacccgagaa ttatcgcggg atgctggcct
     8281 tgttgcggta tgacgacgga acggaaaagt gcgtcggctg cgacctgtgc gaggccgcct
     8341 gcccctcgcg tgtgatccgg gtcgtcagcg gtgaagtgcc gggagaaccg acgaagcggt
     8401 actccaagga atactacatg gatatgacgc gatgcctctt ctgcggcctc tgcgtcgacg
     8461 cctgtcccgt ggatgccctg ggcatgaccc gcgaattcga atgggcggta tacgacaagc
     8521 gccaactcca tctcaataag cagcagctgc tcgccatcgg cgatcgcgcc tatcccgtcc
     8581 gggaaaagcg cctcgagctg cagcacccga acgtggcgtt ttttaacgtg acgttcaagc
     8641 atctcccgca aaaggagaac taggccttcc gcctcgtcct ccgtgctggc cccccacaca
     8701 gagacgaagg aggcaaacag gctgccaccg ggcagagagc cagggcgaca ggggcagggt
     8761 ttcagttgat tcggacgaga taacggactc atggatcaca tttttttctt ttattttgct
     8821 gcggtgatcg ccaccacttc cgtgctggtg gttgccttgc gcaaccccgt gtacagcgcg
     8881 ctggcgttgc tgatcatgtt cttccatgtc gcgggactgt acgtgaccct ccacgcggag
     8941 ttcctggccg cagtccagat cgtcgtctat gccggcgcca ttctggtgct ctacctgttt
     9001 gtcgtcatgc tcctgagcat caagtccgaa gagcgctatc acaaccaact gcccgtcgca
     9061 ggactcctgg gcgtgatgct gtgcacggaa atcatcttgt tgctgattca atcacggacg
     9121 gctgcttcca cccaggcggt ccccgcggac cccgtcacgg gatcaggcaa tacggaaatg
     9181 gtcggggaag ccttgtactc aacctatttg tttccctttg aagtcgcctc gttgatcctg
     9241 ctggtcgcga tgatcggggc cattattctg gcgaaaaaag atatcaacga acaggttcag
     9301 caataaccgg tactgtcatg actgttccct tatcctacta tctggtcctg agcgggtttg
     9361 tgtttctcac cggcgtggtg ggcgtgctga tccggcggaa catcattgtg atcttgctgt
     9421 cggtcgagct gatgctgaat gccaccaaca tcaacttcgt ggccttttcg gaatattttc
     9481 acaatgtcgc gggacaggtc ttcgtgttct tcgccttgac cgtggcggcg gcggaagtgg
     9541 cggtgggtct ggcgatcatt atcgcccttc accggaccaa ttcgtccatc tatatcgacg
     9601 acttgaattt attgaaacgg tagaccaaac gtaccaacca tgatgtacgc gctgatccca
     9661 ttgctaccct tgttctcctt tctgatcgtc ggcatcggcg aacagtggat caaggaccgc
     9721 gcgcatctcg tcgccgttcc ggctatggtg ggctcgttcc tcctgtcgct catggccttg
     9781 cacgacgtcg caacgggcca ggccatcaca gtgaacctgt acacctggct gacctcgggc
     9841 aatctcgata ttcacatcgg catttccatc gatcgcctga ccgccgtcat gctgatcctg
     9901 gtcaccaccg tcagcacgct ggtgcacatc tatacgattg gctatatgca tggcgagccc
     9961 ggctacgccc gcttcttcgc ttatatcgcc ctcttcacct tctcgatgtt gatgctggtg
    10021 atggcggaca acctgctgca gttattcgtg ttctgggagg cggtcggact ctgctcctat
    10081 ctcctgatcg gccattggta tgaacgggcg agcgcctgcg ctgccgcaac gaaggccttc
    10141 cttgtcaacc gcgtcggcga ttttgggttc atcctcggcc tgtttctcgt ctggtactcc
    10201 ttcggctcgc tcgactatgc cacggtcttt gcgcaagcag ggcaattggc gtccaagaca
    10261 acgaaccttc tcggcccgtt cggcggcacc tgggatgttt cggtgatgac gatgatctgc
    10321 cttctgctct ttaccggagc cgtcggcaaa tccgcgcaag tcccgctgca tgtctggctt
    10381 cccgatgcga tggaaggacc gacgccgatt tcggccctga tccatgccgc caccatggtc
    10441 acggcaggcg tcttcatggt cgcgcgattg gc
//
LOCUS       DAOQDS010000099        10470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_3178_length_10470_cov_20.494479, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000099 DAOQDS010000000
VERSION     DAOQDS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10470
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3178_length_10470_cov_20.494479"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..652
                     /locus_tag="PK589_17050"
     CDS             218..652
                     /locus_tag="PK589_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21374.1"
                     /translation="MHGRYFGSRGCLVAGLLPALLTLAGCGASFLEGTIAPPNSSAWT
                     KCGSTTKVRVKPPKSTITVAYTEPTTGTDGKPLTNLAYTTIYYNAGDGPVIAKVVPAS
                     QKTGGAAVSQVILIPTPSQAEQEVTVCVTATDSDDREGPATP"
     gene            778..1302
                     /locus_tag="PK589_17055"
     CDS             778..1302
                     /locus_tag="PK589_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21375.1"
                     /translation="MELRKPDASHPSHEAESERHKHSLEIRLGSNIFRNTNGVIRVQG
                     KEQLVLELAPEQDRILLTIDLYDGSGNHVAHLRRNRWAFNDGNRFSLSTSDSPPTLFP
                     NLPWLKLTDQETGETVLEAAVAPGEKIHVATGKFYSHRGQLIEITSHFCRVGSTHTLF
                     GDVFEARGGTAVLG"
     gene            1318..1731
                     /locus_tag="PK589_17060"
     CDS             1318..1731
                     /locus_tag="PK589_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21376.1"
                     /translation="MTDSPNPSIQAFLVCDSVIEDSLTKKKSLIGIFTHLQAATFPFQ
                     HHQLGLYFCLTDAEGTYHLEIDLVYVNTDQLVCRASLPDIAIHDRLQIADFGINIPAL
                     LFPAPGRYEFRLRMNSRVIAQKDFHVIQLPAPPAA"
     gene            complement(1874..3004)
                     /locus_tag="PK589_17065"
     CDS             complement(1874..3004)
                     /locus_tag="PK589_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21377.1"
                     /translation="MLLVLLALCGLGGMDWAHAEQPASPELCRAQPYEAKMECYQQHL
                     EKVLLAEGTEGALTALEQITTQDPEALREAHPLVHHIGQQSFTRYGTAPLAMSHCRDV
                     FWSGCYHGVLQAFLSSLPAVEPEHILPLCPISETVSAYSFQRYNCLHGLGHGLTIQFR
                     YDVLKSLAFCDALPGSWDRESCYGGVFMENIVTFQQARRAQTASQHHHHEATSFLNPQ
                     DLLYPCSVLTEKYLRACYLMQTSAVLTFLNYDFAQAFTQCARVEGEHQTTCYRSLGRD
                     ISGYTLRDAQQVNALCRLGQGDQVKQCFIGAVKDFILTDANPEPGLALCRSLDGQFKR
                     DCYATVGEMVVPLFDDKNKRVQACHRGENEYVDACLATATAF"
     gene            3216..4919
                     /locus_tag="PK589_17070"
     CDS             3216..4919
                     /locus_tag="PK589_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNJ21378.1"
                     /translation="MSTTPEPPTRSDFIREIVAADRAAGKHDGRVVTRFPPEPNGYLH
                     IGHAKSICLNFGIANEQPGGICHLRMDDTNPTTEDPEYVQAIQEDVRWLGFDWRDKMF
                     FASDYFERLYGFAVVLIKKGLAYVDSLTADEMRRYRGTLTEPGQSSPHRTRSIEENLD
                     LFSRMRAGEFPDGAHVLRAKIDMASPNINLRDPVLYRIRHVAHYRTGTQWCIYPAYDF
                     AHPLSDAIEGITHSICTLEFEDHRPLYDWVVAQCETPQRPQQIEFARLNVTFTVMSKR
                     KLLDLVERKLVNGWDDPRLPTLKGLRRRGFTPEALRAFCEAIGVAKRDAIVEMQLLEH
                     FVREDLNKRAPRVMAVLRPLRLVIENYPEGHVEQLDAINNPEDASAGTRQLPFSRVLY
                     IDQEDFKEDPPKQFFRLAPGREVRLRYAYIITCVGVVKDPTTGDITELRCTYDPETKS
                     GGAQAQRKVKATIHWVSASHAVDAEIRLYESLMLTDPAKIPADQDWTQHLNPRSLERL
                     PSCKVEPSLASIAPGTSVQFERVGYFCADPDSAPDKLVFNRTVTLKDAWAKIEKSHTA
                     R"
     gene            5067..5402
                     /locus_tag="PK589_17075"
     CDS             5067..5402
                     /locus_tag="PK589_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21379.1"
                     /translation="MVTGSGSDDQQEERGSFREQLRRLETAIPGAASSVQPLLMNIHR
                     SVRRVHDQLQHIDVTPLLPRTLVSHVRCFDCGTAKMESFHTSTQGGYHLCPACFQHRL
                     RTGRAKTAH"
     gene            5529..7370
                     /locus_tag="PK589_17080"
     CDS             5529..7370
                     /locus_tag="PK589_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNJ21380.1"
                     /translation="MAPNILLSCESISKSYGVRALFSNLTLALCEGDHVGLIGPNGSG
                     KSTLLKILAGLESPDQGTRTVRGHTRMGYVPQEPSFPAGSSIEHVLQQTLTDAGRDPH
                     EEGGLIARALSIGTFPDPEHCVDTLSGGWRKRLAITQALLLEPDVLLMDEPTNHLDVE
                     GILWLERLLKNRAKAFLVISHDRRFLESIATRMVELNRCYPEGRFEAKGRYSDFLEQR
                     DAALQVQADYQSSLANRVRREVEWLRRGPKARTTKAKGRIQSAGKLIEELDQVEARAA
                     QSSIGIDFSASGRKSKQLLVAKEVTKRFNEKPVVTNLDLLLGPGQRLGLLGPNGSGKT
                     TLLRLLAGTLEPDSGTIMRADGLRIVSFEQHRESLDQDAPLRRALAPSGGDAIVYQGR
                     SVHLASWAKRFLFRPEQLDLPVSRLSGGEQARLLIAQLMLQPADLLILDEPTNDLDIP
                     TLDVLEDSLLEFPGALVLVTHDRWLLDRVSTILLALDGTGRLEWFADYAQWESAQERA
                     AESETGPNEESTTTGDVAGTAPIRKPKKLSYREQKEWGTIEDTILKAEEQVAACQAAM
                     HDPKVMSNAAELQARSEALVAAQAEVERLYARWAELDEKRAETVQSS"
     gene            complement(7758..8318)
                     /locus_tag="PK589_17085"
     CDS             complement(7758..8318)
                     /locus_tag="PK589_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNJ21381.1"
                     /translation="MSNDTVYISFSAEVNQATTEGLLGLCGQLVEKKVSTVYLMLSTP
                     GGTVMNGLNLYNVLRAMPFKLITHNVGNVDSIGNVLFLAGEQRYSSPHATFMFHGVGF
                     DINGAARFEEKTLRERMDSIQADQARIAKILCERTGLTPDEAKQLFLEAVTKDPDYAR
                     TKGIIHDVRELKLPAGAPFLQLVFKR"
     gene            complement(8410..8486)
                     /locus_tag="PK589_17090"
     tRNA            complement(8410..8486)
                     /locus_tag="PK589_17090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8450..8452),aa:Ser,seq:gct)
     gene            complement(8522..9073)
                     /locus_tag="PK589_17095"
     CDS             complement(8522..9073)
                     /locus_tag="PK589_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21382.1"
                     /translation="MGDLEQAIEERKEKVRRLEKEVDQAVELLRQAQHELEILTTARQ
                     IVTKQALLAQWEPDSPQLPLNGPVSPPQSMFHGPKTIHDGTWDYWCERYNKQYTVGEY
                     KALKREMTLVDEVKATLCNSPAPLGPAEIRGELAKTGRVVPQNVMTGVLSRLAKENRI
                     VREGRGKYRLPTDEEVQIQKETA"
     gene            complement(10013..>10470)
                     /locus_tag="PK589_17100"
     CDS             complement(10013..>10470)
                     /locus_tag="PK589_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21383.1"
                     /translation="GHGVLHIFWAWRVPDGERARRFWISQEWLSSQWQALHGAPVVWI
                     KAYQPSHRSRNRLSRYVISQYVQDQCGYVNMCWSWKRSLGFPISRLWEEMRHQWSTRN
                     AYLRIRGEIEIPRIVFLKTWEDLLSGHPIWFSGTILQLVLGKGLVYQEV"
BASE COUNT         2345 a         3164 c         2788 g         2173 t
ORIGIN      
        1 tctgagcctt gagagggctc cgtcctaggc caagctagac gatgggaccg gagaggtagt
       61 ccaactgcag cggaaagaac cggactgtac catagcgagt tttggctttt caaacggcaa
      121 gtcagaagga gcggacaccg gggccacacg aggcaagtat ccatggcatg aaccagacct
      181 gcttgcagag gcttttgaat tagcctagag tacgagtatg catggtcgtt acttcggatc
      241 tcgaggttgt cttgtcgcag gcctgcttcc agccctgctg acgctggctg gctgcggtgc
      301 cagtttcctg gaaggcacga tcgcgccgcc caactccagc gcctggacga agtgcggcag
      361 cacgaccaaa gtgcgcgtga agccacctaa atccactatt accgtcgcct acaccgagcc
      421 caccaccggc acggacggca aacctctgac caatctggct tataccacca tttactataa
      481 cgccggggat ggccccgtga tcgccaaggt tgtgccggca tcacaaaaga ccggcggggc
      541 agccgtttct caagtgatcc tgatacccac gccgagccag gcggaacagg aagtgaccgt
      601 ctgtgtgacg gccaccgatt ccgacgaccg tgaaggcccc gcaacccctt aagcaccgct
      661 agaacagact tgacagcgcc ccgttaaggg cctaccttgc atccgacttg ctcaggccgc
      721 cacacagggc cgaatgcgtc gccaaccggg tcctcaggtc gcactagatt tctacgcatg
      781 gaactacgca aacctgacgc gtcacatcct tcccacgagg ccgaatcaga acggcacaaa
      841 cacagcttgg agattcggct cggctccaat atcttccgca ataccaacgg cgtgatccgg
      901 gttcagggca aggaacaact cgtcttggaa ttggcaccgg aacaagatcg catcctgctg
      961 acgatcgatc tgtatgacgg gagcggcaat catgtggcgc atctccgccg caatcggtgg
     1021 gcgttcaacg acggcaatcg cttttcgctc agcacaagcg attccccgcc gaccctgttt
     1081 cccaatcttc cctggctcaa gctgacggat caggaaaccg gcgaaacggt tctggaggca
     1141 gcggtggcgc ccggcgaaaa gatccacgtg gcgacgggca aattttattc tcaccgcggt
     1201 caactcatcg aaatcacttc gcacttctgc cgcgtcggct ccacgcatac cctgttcgga
     1261 gatgtgttcg aagcccgcgg cgggacagcg gtgctcggct gacgccggcc gaatcgtatg
     1321 actgacagtc cgaaccccag catccaggcc ttcctggtct gtgacagtgt catcgaagac
     1381 agcctcacca agaaaaaatc tctcatcggc atcttcacgc atctgcaggc cgcgactttt
     1441 ccatttcagc accatcaatt gggcctgtac ttctgcctga ctgatgcgga aggaacgtac
     1501 cacctggaga tcgatctggt gtatgtgaac accgatcagt tggtctgccg cgcctcactt
     1561 cccgacatcg ccattcacga tcgcctgcag attgcggatt tcggcatcaa cattcccgct
     1621 ctgctgtttc ccgctccggg ccgttatgag tttcgcctgc gcatgaacag tcgtgtcatt
     1681 gcgcaaaaag acttccacgt catccaattg ccggcgccac cggctgccta atctggccgg
     1741 ctcactagcg cacgaaggca tacgcgatca ggatgtcagg catccgaacc cgaatccgct
     1801 agaggcagct cgcgctcacc tggacggggt gttgaggagg gccagccgca aaggacaggc
     1861 gatcggctag tagctagaag gccgtggcag tggcaaggca ggcgtcgaca tattcgtttt
     1921 cgccgcgatg gcaggcctgt acgcgcttat ttttgtcgtc aaacaggggc acgaccattt
     1981 ctcccacggt ggcataacag tcccgtttga actgtccatc caggctgcgg cacagcgcga
     2041 ggccgggctc gggattggca tcggtcagaa tgaagtcctt caccgcaccg atgaaacatt
     2101 gcttcacctg atcgccctgg ccaagccggc acagagcatt gacctgctgg gcatcacgca
     2161 acgtgtagcc actgatatcc cgccccagac ttcgataaca ggtcgtctga tgctccccct
     2221 ccactcgagc gcattgcgtg aacgcctgcg caaagtcgta attcaggaag gtcagcacgg
     2281 cagaggtctg catcaggtaa caggcgcgca gatatttttc cgtcaggacg gagcagggat
     2341 acaacaaatc ctgcggattc aaaaaactgg tcgcttcatg gtgatgatgc tggctggccg
     2401 tttgcgcgcg acgggcctgc tgaaacgtga cgatgttctc catgaacacg ccgccataac
     2461 aggattcgcg gtcccagctg cccggcaagg catcacaaaa cgcgaggctc ttcagcacat
     2521 cgtagcgaaa ttgaatggtc aggccgtggc ccaagccatg aaggcagttg taccgctgaa
     2581 acgaataggc cgaaaccgtt tcgctgatgg ggcagagcgg aaggatatgc tccggttcca
     2641 cggcgggcag actgctcagg aaggcctgta ggacgccatg atagcaacca gaccagaaga
     2701 catcccggca gtgggacatg gcgagcgggg ccgttccata gcgcgtgaat gactgttggc
     2761 ctatgtggtg gacgagcggg tgcgcctcgc gcagcgcttc cggatcctgt gtcgtgatct
     2821 gctcgagagc cgtcagcgct ccttcggtcc cctcggccag taacaccttt tccaagtgct
     2881 gctggtagca ttccatcttc gcctcgtacg gctgcgcacg gcagagctcg ggggatgccg
     2941 gctgttccgc gtgggcccaa tccatcccgc ccaggccaca cagcgccaga agtaccagaa
     3001 gcattcgttg accacgcatc atccaatccc ccttactgaa tcactacacg tcgggacatc
     3061 tgccataaaa aagtgattct aaaatactcc tccccatcga tccagagaaa atcaacacgg
     3121 cacaggccgt tcatcaatcc cccttctccc gcctccagtt ctatgataga accattcgcg
     3181 ttcatcaccg gcaactcgac ggaatcgcgc ccagtatgtc caccacgcca gaacctccga
     3241 cacgctcaga cttcattcgc gagattgtgg cagccgatcg tgctgccggg aaacatgacg
     3301 gccgagtggt gacccgcttt ccgccggaac cgaacggcta tctccatatc ggccatgcca
     3361 agtcgatttg cctcaacttc ggcattgcca acgagcaacc gggtgggatc tgccatctcc
     3421 gcatggatga tacgaacccc acgacagaag acccggaata cgtccaggcg attcaagaag
     3481 atgtccgctg gctggggttc gactggcgcg acaagatgtt tttcgcctcc gactatttcg
     3541 agcggctcta tggctttgcc gtcgtgctca tcaagaaagg cctcgcctac gtcgatagcc
     3601 tgacggcgga cgagatgcgc cgttatcgcg gaacgctgac ggaaccggga cagtccagcc
     3661 cgcatcgtac ccgcagcatc gaggaaaacc tggacctgtt cagccgcatg cgcgcagggg
     3721 aatttcccga cggcgcccat gtgcttcgtg ccaagatcga catggcctcg ccgaacatca
     3781 atctccgcga tccggtgctc taccgcatcc gccatgtggc gcactaccgc accggcacac
     3841 agtggtgcat ttacccagcg tatgatttcg cccatccgct gtccgatgcg atcgaaggca
     3901 ttacccattc gatctgcacg ttggaattcg aagatcatcg tcccctgtac gactgggtgg
     3961 tggcgcaatg cgaaacacct cagcggccgc agcaaattga atttgcgcgg ctgaatgtga
     4021 ccttcacggt catgagcaag cgcaaactcc tcgatctcgt cgaacgcaaa ctggtcaacg
     4081 gctgggatga cccgcgcctg ccgacactca aaggactccg ccgacgcggg tttacgcctg
     4141 aagcgctccg tgcgttttgc gaagccattg gcgtggcgaa gcgggatgcc atcgtcgaaa
     4201 tgcagctgct tgaacacttt gtccgcgaag acttgaacaa gcgcgccccc cgggtcatgg
     4261 ccgtgctccg tcccttgcgc ctcgtgatcg aaaactatcc cgagggacac gttgagcagc
     4321 tcgatgccat caacaatccg gaagatgcgt cggctggaac ccggcagttg cccttttccc
     4381 gcgtgctcta catcgaccag gaagatttca aagaggatcc acccaagcaa ttcttccgcc
     4441 tcgcacctgg gcgcgaagtc cgattgcggt atgcctacat catcacctgc gtcggcgtcg
     4501 tgaaggatcc gaccaccggc gacatcacgg aacttcgatg cacctacgat cccgaaacca
     4561 agagcggcgg agcgcaggca caacgaaagg tgaaggcgac gatccactgg gtgtcggcca
     4621 gccatgccgt cgacgcggaa atacggctct atgaaagtct catgctgacg gatccggcca
     4681 aaattcctgc cgatcaggat tggacgcagc atctgaatcc ccgttcgctg gaacggctgc
     4741 catcgtgcaa agtggaaccc agtctggcct caatcgctcc cggcaccagc gtccagtttg
     4801 aacgcgtcgg ttacttttgt gccgaccccg attctgcgcc ggacaaactg gtgttcaatc
     4861 gaactgtcac gctcaaagac gcctgggcga aaatcgagaa atcccacacc gcccgttgac
     4921 cagttccttc ctccttcagt cagcgtcatt cccccgcaaa gtactggcgc gccaaccttc
     4981 tgcctccttg aaccctcacc tgtcgatgaa cgagaatcac gcctcacgca gacggtgaat
     5041 tcatacctag caacggggtg gttacgatgg tgacaggatc cggcagcgac gatcagcaag
     5101 aagaacgcgg ttcattccgg gagcagcttc gacgattgga aactgcgata ccgggcgccg
     5161 cgagtagcgt gcaaccactc cttatgaaca ttcatcgctc cgtccgccgg gtccacgacc
     5221 aacttcagca catcgatgtg acgcctctcc tgcctcggac gctcgtcagc cacgtgcgct
     5281 gctttgattg cggcaccgcc aagatggaat cgtttcatac ctccacgcag ggcggctatc
     5341 atctttgccc cgcctgcttt cagcatcggc tccgcacggg acgcgccaag accgctcatt
     5401 aaccattcca ctcgccgttg atctcgacgt cgcgttaaca caggaagcgg cactcctgcc
     5461 gctagcccta ggcccttccc ccaccgtata ctgcacgctt tcactgttgc gagagaggta
     5521 atcagggtat ggcccccaac attctattga gctgcgaatc gatcagtaaa agttatgggg
     5581 tacgagccct gtttagcaat ctcacgctgg ccttgtgcga aggtgaccat gtcggcctga
     5641 tcggcccgaa cgggtccggc aaatccacgc tgcttaaaat cctggccggt ctcgaatcgc
     5701 ccgaccaagg cacacggacc gtacggggtc atacccgcat gggatatgtc ccgcaggagc
     5761 caagctttcc tgccggctcg agcatcgaac atgtcctcca gcaaaccctc acggatgccg
     5821 gtcgtgaccc tcacgaagaa ggcggcctca tcgcccgagc cctcagcatc ggcacctttc
     5881 ctgatccgga acactgcgtc gacacccttt cgggagggtg gcgcaagcgc ctggccatca
     5941 cacaggcttt gctgctcgag ccggacgtgc tgctcatgga cgagccgacg aaccatttgg
     6001 atgtcgaggg cattctttgg ctggagcgcc tgctgaagaa ccgcgccaag gcctttctcg
     6061 tcatcagtca cgaccgtcgt tttttagaat cgattgcgac gcgcatggtg gaactgaacc
     6121 gttgttatcc cgaaggccgc tttgaagcca aaggccgata cagcgacttt ttagaacaac
     6181 gcgatgccgc actccaggtg caggcggact atcaatcgtc gctggccaac cgtgtgcggc
     6241 gcgaagtgga atggctgcgc agagggccca aagctcgtac caccaaagcg aagggccgca
     6301 ttcagtcggc cggcaaactc attgaggaat tagaccaggt cgaagcccgc gccgcacaat
     6361 cgtcgatcgg catcgacttt tccgcctccg ggcgaaagtc caaacaactg ctggttgcga
     6421 aagaggtcac gaagcgcttc aacgagaagc ccgtcgtcac gaatctggat ttgcttctcg
     6481 ggccggggca acgcctgggc ctccttggcc cgaacggcag tgggaagacc actctccttc
     6541 gattactcgc cggcacgctg gaacccgact caggcacaat tatgcgcgcc gatggactgc
     6601 gcatcgtttc cttcgaacaa catcgtgaat ccctggatca agatgctccg ctgcggcgcg
     6661 cgctggctcc ttcaggcggc gatgcgatcg tctaccaggg ccgctcagtc catcttgcgt
     6721 cctgggcgaa gcgctttctc tttcgacctg aacagctcga ccttccagtc tcccggcttt
     6781 cgggcggtga acaggcgcga ctgctgatcg cccaactgat gttgcaaccg gccgacctgc
     6841 tcatcctcga cgaaccgacc aacgatttgg atattccgac actcgatgtg ctggaagaca
     6901 gcttactcga attccccggc gcactggtgc tcgtcaccca tgatcgctgg ttgctggatc
     6961 gtgtctctac cattcttctg gcactggacg gcaccggtcg cctcgaatgg ttcgccgact
     7021 atgcccagtg ggaaagcgcg caggaacggg ctgccgagtc cgaaacgggc ccgaatgagg
     7081 aaagcaccac gaccggcgat gtggcaggaa cagcacccat tcgcaagccc aagaaactca
     7141 gttatcggga acagaaagag tggggcacga ttgaagacac gatcctgaag gccgaggagc
     7201 aggtggccgc ctgtcaggcc gccatgcacg atccgaaggt catgtcgaac gccgcggaac
     7261 tacaggcacg gagcgaggcc ctggtggcgg cgcaagctga agtggaacgg ctctacgccc
     7321 gctgggccga gttggatgaa aaacgcgcag agaccgtgca aagttcgtga agggaatatt
     7381 tggctggggg actaggaatc gaacctaggt agccagctcc aaagtcgcaa aaagcaccct
     7441 agagacggtg aaggatctta gaggaaggat tgccattttt cccttatgaa acaaaatctt
     7501 caccaacgat cgatgagatt agttacgatt agtgtacacc tgtaatcgtg ccctaaaccg
     7561 tgactgaacc gtgaccaaaa ccgtgaccgg ggacttatca aaaattggac acaattttga
     7621 agatgaatca tgaaatggct cacggactat atgcaggtcg aagtagaatt ttcactatcg
     7681 attgaccatc ctctctcctt cagagtatca tcagtttcca cactaaaggt gtaatcatga
     7741 tcaatgataa cgacgcttta ccgcttgaat acaagctgaa ggaagggcgc gccagcgggc
     7801 agcttgagct cacggacatc gtgaataatc cccttagtgc gggcgtagtc cgggtctttg
     7861 gtgacagcct ccaaaaacaa ctgtttcgct tcgtcgggag tgagtccggt tcgctcacag
     7921 agaatctttg caatacgagc ctgatccgcc tggattgagt ccattctctc gcgcagggtc
     7981 ttttcttcga atcgtgctgc gccgttgata tcaaacccca caccatgaaa catgaaagtc
     8041 gcatgagggc ttgaataccg ttgttctcca gcgaggaaca agacgttacc tatagagtcg
     8101 acatttccga cattatgagt aataagcttg aagggcatgg cacgaagcac attgtaaaga
     8161 tttagcccat tcataactgt gccacccggc gttgaaagca taagatagac cgtggaaacc
     8221 tttttctcga ctaactgacc acatagaccc agtaaacctt ccgtggtcgc ctgatttact
     8281 tccgcgctaa aagagatgta aacggtgtca ttcgacataa aaccctcctt ggtaagttga
     8341 tatgattgtt gacaaaccag gagggatcag gcaggataac aacagcctac agctctctcc
     8401 ggttcgattt ggtgcccctg gccggaattg aaccgacgac aggcccgtta gcggcggggt
     8461 gctctgtcca gctgagctac aggggcggac gttagagtaa accgcctgat ggtagcagcc
     8521 atcaggcggt ttctttttga atctgtacct cctcatctgt cggaagacga tactttcctc
     8581 ttccttctcg cactattcgg ttctccttcg ccaaacgaga caggactcca gtcattacat
     8641 tctgcgggac aactcgtccc gtttttgcca gctctcccct tatctccgcc ggccccaacg
     8701 gagccggtga attacaaagc gtagccttaa cctcatccac taatgtcatc tcacgtttca
     8761 gcgccttgta ctccccgacg gtgtactgct tattgtacct ttcgcaccaa taatcccagg
     8821 tgccgtcatg tattgtcttg ggaccgtgaa acatactttg aggaggggag acggggccat
     8881 tcagcggaag ctgcggggaa tccggttccc actgagccag aagagcttgc ttggtgacaa
     8941 tctgcctggc ggtcgtaagt atctccaatt cgtgttgagc ctgacgcagt aattcgacag
     9001 cttgatcgac ttccttttca agccggcgca ccttctcttt acgttcttct attgcctgtt
     9061 caagatcacc cataccgctt acgttacacg acactgtgct actttgcaaa tatttttcac
     9121 agactacagt agccttgtag ctaatcttag cataactaat tgataatgct agttatttta
     9181 taattattca atgctacaaa ttttaatgtt tcaccgctcc attgtcaaat ctgggcggcc
     9241 tacgagatca tatccctccg aaggaagccg atgtcgccag cccgctcacg agattttttc
     9301 ggtgtgcggc atcagctcgg cgacctgcaa caaatagaaa gttaatgctg cagtcttggt
     9361 gtgacctcgg ctcgctcccc ttccggtctg agacttcgcc gagagggaga aggcatgccc
     9421 catgagcctt aacttccgtt acccaactcc ttcagccgac acccgttgac gttgcgtcct
     9481 actttgacca tatagatcaa gtgaagcggg ggctgcgccc ccgatccccc cggccgtccg
     9541 gtccagtggt ccggagcggc cgggcggcct cgttgggccg agtctttgcg ttcgtaatct
     9601 ccccatggtt tcagtattcc tcttctctac tctgtctggg taagcaacga tccaacagaa
     9661 tggcggccaa gctgacgcat cgccgccctg catatggacg gagcatcgtt ccctctcacc
     9721 cgtgagggat agtggacggg tgacagggag aaatggatta gagtgtttca ccgtcgctcc
     9781 gtcattcccg cacagtcgat acaaagccag atttcgcacg tcatgtcgtt gtctcgataa
     9841 cagaagcgta cccacgctcg ttcgaattga cagaactcac acagccgttc ctttgtatgt
     9901 tcaggacaga gccaagcctt tctcgcccga tcgtacgtgg tcgcttcccg tccgcaacag
     9961 gacggaccga agagacttgg cagcttcaga tcgcatcgat gccacatcat tctcacacct
    10021 cctgatagac cagccccttg cccaacacga gctgcagaat cgtgccgctg aaccaaatcg
    10081 gatggcctga gagcagatcc tcccaggtct tcagaaagac gattcgaggg atttcgatct
    10141 cgcccctaat ccggagatag gcgttccgag tggaccattg gtgccgcatc tcttcccaca
    10201 gtcggctgat gggaaagccg agcgagcgtt tccacgacca acacatattc acgtagccgc
    10261 attgatcctg gacgtattgg ctgatgacgt agcggctcag acggttccgg gaacgatgac
    10321 tgggctgata ggccttgatc cagaccaccg gagcgccatg gagggcttgc cattgtgacg
    10381 agagccattc ctgggagatc cagaagcgcc gggcacgttc cccatccggc acccgccagg
    10441 cccagaagat atggaggacg ccatgcccct
//
LOCUS       DAOQDS010000100        10420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_3208_length_10420_cov_19.234636, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000100 DAOQDS010000000
VERSION     DAOQDS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3208_length_10420_cov_19.234636"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            574..1563
                     /locus_tag="PK589_17105"
     CDS             574..1563
                     /locus_tag="PK589_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21384.1"
                     /translation="MTAYTADSAEAQVPPAQSSSVAQVDLKISEWFSQGETVWSHNAS
                     GLDPNLGNPSSKLKYKDTGTNVTEITGRVQLKNKMFFRGAFGYGAIGGGRLTDDDFLS
                     AQGAASQGATVSGEHRFSRTYSDIGGDNLWYLNGDVGATAHTFQDNKGTLGVFVGLQY
                     WRERHVATGVTQAECTTASAPSSEFRCSPAGTVGFRNQAVITNTTTWFSGRLGGELEY
                     KFDRRISVEAKVAMLLSYLDNEDVHHLRTDLAQDPSFRMTGLGIGTTSDFNLGVKLWD
                     RLYLTGGYRLWWNRVLVNDQWKLYGSDGSTASAPLTQFQTLRHGPTVGLTYTF"
     gene            complement(1747..2817)
                     /locus_tag="PK589_17110"
     CDS             complement(1747..2817)
                     /locus_tag="PK589_17110"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNJ21385.1"
                     /translation="MNTHELERLLMQQPVSLLHRLARGRISRHFRSGKRKLVDLLLQA
                     SAENRPGLESDLQTLIDERAQRPQPVPVEKTRPPEARSRQAVPATSRPHRTDDHGHHH
                     EPPVSLHEWLSGIGVPPPQPFVPDAWQVEALAKLAETDVVVSVPTGSGKTYVAIEATA
                     RAMRENRTVIYTSPLKALSNTKFTEFSRVFGPGQVGILTGDRREHSQAPLLIMTTEIL
                     RNLLYDAAGGEIDVRLDTLGLVIMDESQYLADPERGVVWEETLIFCPSQARLLLLSAS
                     IGNPQDIADWLTAIRPSPCSLIRHTKRSVPLRAGYLHPNEKLTPLFRTAGIPYGQPHL
                     LHPEAKRLFMEYEEETGPSRSR"
     gene            complement(2814..4676)
                     /locus_tag="PK589_17115"
     CDS             complement(2814..4676)
                     /locus_tag="PK589_17115"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNJ21386.1"
                     /translation="MPPSIHPVDLVATLRQRNLTPAIIFLTSRRACDEAMQSFERSQV
                     TLPKAQQEQIARVLTQVTEQFPSIAEHPLIETVQRIGVAAHHAGHLPSWKIAVEELMR
                     TGSLDAVFATTTLAAGVDFPARTVVVTQSSIRKARDFTDLTASEIQQLAGRAGRRGKD
                     LVGFAVITPSPYIDLTVLTKGLTGQPEAIHSQFVISYPMVLNLLKAHPREQIQSILAK
                     SFAQFQLNQRTAVLETKLDSLRAEMEPFGPRVCSDWITQWQVFEQARKRKKPRSPFTR
                     QESPDVAARLHFLTPGRVVGVTRGRGIILRQYRSKGQHAPMVTLLREGGSLNECPASI
                     VTQVFDRTFDCEETSTYPWCTPQALEQLTHHLTDLPPRLPILPILAHQHEETPIDSSI
                     IQTLGDFPCPTCPSRQACQRDFPRASRVRQEIQRHIKSIQALQTSLWHRFQERIDVLE
                     KFGYLHANAQLTADGEWARLIRIDHSLLITELIRAEAFGAIDPPLLAAVMASIAHDDD
                     RPGSFPRGSAGLVTLLFQVRKLAESLTPHEAPPMLRADVAALTERWVSDQTLTWIGLA
                     RLTTMAEGDMFRLFARTIEFLSQLKTLKATHPSLAESADHAITAMRRGVLEELP"
     gene            complement(4651..5889)
                     /gene="glgC"
                     /locus_tag="PK589_17120"
     CDS             complement(4651..5889)
                     /gene="glgC"
                     /locus_tag="PK589_17120"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNJ21387.1"
                     /translation="MANIFTMVLAGGKGERLQPLTDQRAKPAVPFGGKYRIIDFTLSN
                     CLNSGLRQIAVLIQYKSHSLDRHIRTGWNILNPELGEYIVSVPPQQRISEEWYRGTAD
                     AVYQNLFLMDNDHPEFLLVLAGDHIYKMNYADMYNLLIEKQADAIVGAIETPIADANR
                     FGVIAVDEDRRILSFDEKPEHPMHIPGDPTQAFVSMGIYLFRTDVVREQLIRDAKEGT
                     KHDFGKNIIPRMIKECRVYAFKFQDENKKAVNYWRDIGTLDAYWEANMDLVSVDPQFN
                     LYDQHWPIRTYQGQFPPAKFVFAQDFQGGRMGVALDSIVCGGCIISGGRVQNSVLSPH
                     VRVHDHADVRESVVMENVVIGEHARIRRAIIDKDVVIPPKTVIGFDPVADRQRFKVTD
                     SGLVVISKGMKLHAAVDPSG"
     gene            6024..6302
                     /locus_tag="PK589_17125"
     CDS             6024..6302
                     /locus_tag="PK589_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21388.1"
                     /translation="MKKQNARPAIMKPAIEPANPMMEMVWRLERLERQVQRLSELVRN
                     HAEDNDRLVRIVAEDRDVIRRELLRKHEHRVRVRKHLRDVSSKVDLEH"
     gene            6426..7478
                     /locus_tag="PK589_17130"
     CDS             6426..7478
                     /locus_tag="PK589_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ21389.1"
                     /translation="MRHHGPVAAVDHVSFQVRRGEFFSILGPSGAGKTSVLRIVAGFE
                     DPDAGDVLIDGRSMLGVPPNRRPVNLVFQSYALFPHLTVFENVAFGLRMRRVPAALIA
                     EQVEQALAMVKLVGKERRKPAQLSGGEQQRVALARALVNKPAVVLLDEPLAALDQQLR
                     QAMQVELKSIQEQAGLTCVCVTHHQEEAMMMSDRIAVMHQGRMWQVGTPREVYENPRN
                     LFVSRFLGVSNELPGRVDDVVEDGHLFHPADDQVPAFPVRAGSEASPGHSATWSIRPE
                     RLRMSRERPAGRDAVLNGFIEKVLFAGSETKYLVRVGRDRLWEARTTGAGESPHAPGD
                     PVFLHWALGDGRVFVE"
     gene            7481..8371
                     /locus_tag="PK589_17135"
     CDS             7481..8371
                     /locus_tag="PK589_17135"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ21390.1"
                     /translation="MPSDDVSHVSPASQDSCRSCWLAAPGLLWLALFFLVPLVLVFAI
                     SFASRGTYGGIVWEFTWANYVDLLHPLYGKILGQSLWYATLTTAICFLLGFPLAYVIA
                     RSPARWQPVLLLLVMLPFWTNFLVRTYAWMIMLRHDGLANNLLLGLGLITEPLELLYT
                     PTAVVIGLVYGYLPFMVLPLYVAVERLDPVLVEAAWDLYASRWAVVTRVVIPLTMPGI
                     VGGCILVFIPSIGSFITPDLLGGARSMMIGNLIQHEYLVVRDWPLGSAVSFVLMAIVM
                     AAVVLYYRQASPGDGPLEGR"
     gene            8368..9141
                     /locus_tag="PK589_17140"
     CDS             8368..9141
                     /locus_tag="PK589_17140"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ21391.1"
                     /translation="MKQRSLGLNAVSLLAMLFLYGPIIVLVLYSFNAAHLSMTWRGGT
                     LKWYAALVHDEALLAATANSLLVALVSTIGATGLGGLLALGMEQMPHRRQQLLEGGLV
                     LPLVIPEVMMGVALMLLFVTVRVPLGLTTVILGHIVFNIPLVTIMIRTRLRKLDPMLR
                     EAAADLGADSWQVFRHVTLPLLRPAIWGAVLVAFTVSLDDFLVTFFTAGPGSTTLPLK
                     VYSMIKSGVTPEINALSALLLVVSMIGVAASLYLQRREV"
     gene            9187..10212
                     /locus_tag="PK589_17145"
     CDS             9187..10212
                     /locus_tag="PK589_17145"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNJ21392.1"
                     /translation="MWLTGYLTGCGQRFDENRVETRPVLHYFTWSDYVSPDIIQEFER
                     REQVKVVIDTFSSNEELLAKLQSGATGYDVAVPSDFMVAIMIQQGLLSELDQAALSNT
                     SQLEPHLQTLPFDPERRYSVPYLWGTVGIGYDSASIPTPPDSWSILWDARYKGRISML
                     NDQREVFGAVLRSMGQSMNSTEPQVIESAKDRLLNQKPLVKTYTSDHYDQLLASGEVV
                     LAHGWGGQVARAMADRPSIRYVVPKEGATIWADCLVVLRTAPQKALAMRFINYLMEPT
                     VAARTSERLRFASTSRSARELVDAATRDNPAVYPSLDQLARLEWMRDVGKGIRLYDRA
                     WTELKMQ"
BASE COUNT         1956 a         2895 c         3285 g         2284 t
ORIGIN      
        1 gaaatgctgt gcggtcatag tcagtactcc cttaggtgat cttcctgaat tgaaaaatgg
       61 aaacaattgt tggtgagcaa caggcgtgcc acaacagatt gcttcggcgt tcttcttagt
      121 tgattgtcgg gaatcgctca gcggagttcg gtgagaatcc cgtcaggata tcttgcagag
      181 ccgaccctaa cggatcaaac gcgaataggc aagcaaaaat ttcactcgtc gcacgtatct
      241 tgcgtgggcc gttctctcgt acaaatgctc taggaaattt tttccgcctg tagggtcgtg
      301 gcacttcgtc gtacaaaatc ggaacgcctg tcttgcgacg atgaatcctt tccagtcgtc
      361 ctcggacgat cgcatcagga gagtcggtta tagagcccac tagggccgag aaagttgttg
      421 accatgcacg ataggtaggg tatctgttgg cgcgctgttc cggccgggat acgtagtctc
      481 gcggtcatgc gaacgagtct ttggcccgaa tggttaggga gtgccccacc tatgatcccg
      541 ctacgatgtc tgttctgtgc gatatgcctg tgtgtgaccg cgtacactgc tgattcggcg
      601 gaggcgcagg ttcctccggc ccagtcctcg tcggttgcgc aagtcgatct caaaatttcc
      661 gagtggttca gtcaaggtga aacggtttgg agccacaatg cctcgggcct ggaccccaac
      721 ctgggcaatc ccagttcaaa actgaaatac aaagataccg gcaccaatgt caccgagatc
      781 accggccgag tgcagttgaa aaataagatg tttttccgcg gcgcctttgg gtacggggct
      841 atcggcggcg gccgtcttac ggatgatgat tttctgagtg cccagggagc tgcctctcaa
      901 ggggcgaccg tgagcggaga acatcgtttt tcgcgaacct acagcgatat cggcggtgac
      961 aatctctggt acctcaatgg cgatgtgggc gcgacggccc ataccttcca ggacaacaag
     1021 ggaaccctgg gggtgtttgt gggattacag tattggcggg aacggcatgt ggccacgggc
     1081 gtcactcagg cggaatgtac gaccgcctca gcacccagtt cggagtttcg ctgttctcct
     1141 gctggaacgg tcggatttcg caatcaggca gtcattacca acacgaccac atggttttct
     1201 ggtcgccttg gtggagagct ggaatataag ttcgatcgtc ggatcagcgt cgaggccaag
     1261 gtggcgatgc tgttgtcgta tttagataat gaggacgtac accaccttcg cacggacctt
     1321 gcgcaagatc caagttttcg aatgaccggc ctgggaatcg gcaccacgtc ggactttaat
     1381 ctgggagtga agctctggga tcgtctgtac cttacgggtg gttaccgcct gtggtggaat
     1441 cgcgtgctcg tcaatgatca gtggaaattg tacgggtcag acgggtctac ggcctccgcg
     1501 cccttgaccc aattccaaac gcttcgacat ggcccaacgg tgggcctgac ctatacattc
     1561 taagggacga agaggctctc ccgtgacgtc gatttctgcc gcctcctcgc acagccccac
     1621 gtacaggatg cgttcgacga gaaccctgcc ctcagatccc acagctgacc gttgatcaga
     1681 agactttgcc ggccgactgc aaagcggaaa gggctggaac ggcaccggtt cttcggcgcc
     1741 tgagggtcag cgtgagcggg aggggccggt ctcttcttcg tattccatga acagccgttt
     1801 ggcttcgggg tgcaagagat ggggctgacc gtacggaatg ccggcagttc gaaacagagg
     1861 cgtgagcttc tcgttggggt gaagataacc ggcccgcagc ggaactgatc gcttggtgtg
     1921 tcgaatgagc gagcaggggc tcgggcggat cgcggtcaac cagtcggcaa tgtcctgcgg
     1981 attgccgatt gaagcggaga ggagcaggag gcgagcctgc gaggggcaaa aaatcaaggt
     2041 ttcttcccac accactcctc gttcgggatc ggccaggtat tgggactcat ccataatgac
     2101 cagtcccagc gtgtctaacc gcacatcaat ctctccgccg gctgcgtcat acagcagatt
     2161 gcgcaggatc tccgtcgtca tgatcaagag gggagcctga ctatgttcgc ggcggtcgcc
     2221 ggtcaggatg cccacctggc cagggccgaa tacgcgtgag aactcggtaa atttcgtgtt
     2281 ggaaagcgcc ttgagagggg aggtatagat gacggtgcga ttctcgcgca tcgcgcgagc
     2341 ggtcgcttca atcgccacgt aggtcttgcc gctcccggtc gggacgctca cgaccacatc
     2401 ggtctctgcc agttttgcca gcgcctccac ctgccacgca tcgggaacaa acggttgcgg
     2461 cggcggcacg ccaatccctg acagccattc atgcagcgag acaggcggtt cgtgatgatg
     2521 gccatgatca tcagtccggt gcggacgcga cgtcgcaggg acggcttggc gtgatcgtgc
     2581 ttcaggtggt cgtgtttttt cgacgggcac tggttgtggg cgctgcgccc gttcgtcgat
     2641 gagggtctgc aggtcggact ccagtcccgg gcgattttcg gcggacgctt gaaggagcaa
     2701 atcgaccagc ttgcgttttc cggaacggaa atgccggctg atccgtcccc gcgccaatcg
     2761 atggagcaga gagaccggtt gttgcatcag cagccgttcg agttcgtggg tgttcacggc
     2821 aattcctcca gcacaccccg gcgcattgcc gtgatcgcgt gatcggctga ttccgccagc
     2881 gaaggatggg tcgcttttaa tgtcttcagt tgggacaaaa actcgattgt acgcgcgaag
     2941 agccggaaca tgtcgccttc cgccatcgtc gtcaggcggg ccagtccgat ccaggtgagc
     3001 gtctgatcgg aaacccaccg ttcggtgagc gccgccacgt cggcccgcaa catcggcggc
     3061 gcttcgtggg gcgtcaagct ctccgccagc ttccgcactt gaaacagcaa ggtcacgagc
     3121 ccggcgcttc cacgcggaaa cgatccggga cgatcgtcgt catgggcaat gctggccatg
     3181 acggccgcca ggagcggggg atcgatggcg ccgaaggctt ccgcccggat caattcggtg
     3241 atcagcagcg aatgatcgat ccgaatcagc cgcgcccatt ccccgtcggc ggtgagctgg
     3301 gcattggcat ggaggtagcc gaatttttcc aggacgtcga tacgctcttg aaaccggtgc
     3361 cacaaactgg tctggagggc ctgaatcgat ttgatatggc gttggatctc ctggcgcacc
     3421 cgcgaggcgc gtgggaaatc acgctgacag gcctggcgcg aggggcaggt cgggcagggg
     3481 aagtccccca gcgtttgaat gatggaactg tcgatcggcg tctcttcgtg ctggtgcgcg
     3541 agaatcggaa ggatcggcag cctcggcgga agatcggtca gatgatgggt cagttgttcc
     3601 agcgcctgcg gcgtacacca gggataggtt gatgtctctt cgcagtcgaa cgtccgatcg
     3661 aagacctgtg tcacgatgga ggccgggcat tcattcagcg agccaccttc ccgcaggagg
     3721 gtgaccatcg gcgcatgttg gcctttgctg cggtactggc gcaggatgat cccgcgtcca
     3781 cgggtgacac ccacgacgcg cccgggcgtc aggaagtgga ggcgcgccgc aacatcgggc
     3841 gattcctgcc tggtgaacgg gctccggggc ttcttgcgtt tccgcgcctg ctcgaacacc
     3901 tgccactgcg taatccaatc tgagcagaca cggggaccga acggctccat ttcggcgcgc
     3961 aggctatcga gtttggtttc gagcacggcc gtgcgctggt tcaactgaaa ttgggcaaag
     4021 cttttggcca ggatggattg aatctgttcg cgtggatgcg ccttgagcag gttcaagacc
     4081 atggggtaac tgatcacgaa ttggctgtgg atcgcctccg gctgccccgt caggcccttg
     4141 gtcaggaccg tgagatcgat atagggagac ggggtgatga ccgcgaaccc gacgagatct
     4201 ttgccgcggc gaccggctcg gccggccaat tgctgtattt cgctcgcggt gagatcggta
     4261 aaatcgcgcg ctttacgaat gctggattgc gtgaccacca ccgtgcgggc aggaaagtcc
     4321 acgccggctg cgagcgtggt cgtggcaaag acggcatcca gcgatcccgt gcgcatcaat
     4381 tcttcgacgg caattttcca ggagggcaaa tgtccggcat ggtgcgcggc cacaccgatg
     4441 cgctggaccg tctcgatcag cggatgttcg gcaatgctgg gaaactgctc ggtcacctgc
     4501 gtcagcacgc gggcaatctg ttcctgttgc gctttgggca gcgtcacctg actgcgttcg
     4561 aaggattgca tcgcttcgtc gcaggcccgt ctcgaggtga gaaaaatgat cgcaggggtg
     4621 aggtttcgtt ggcggagggt cgcgaccaga tcaaccggat ggatcgacgg cggcatgcaa
     4681 tttcattccc tttgagatga ccaccaatcc cgaatcggtg actttgaaac gttgacgatc
     4741 cgccaccggg tcgaacccga tcacggtttt aggcggaatc acgacatcct tgtcgatgat
     4801 ggcgcgccgg atacgggcat gttcgccgat gaccacgttt tccattacga cagactcgcg
     4861 tacgtcggca tgatcgtgca cacgcacgtg cggcgacagc accgagttct gcacgcggcc
     4921 gcctgaaata atgcagcccc cgcagacgat cgaatccaat gcgaccccca tccggccgcc
     4981 ctgaaagtcc tgtgcgaaga cgaacttggc agggggaaat tgcccttggt aggtgcggat
     5041 gggccaatgc tggtcgtaca ggttgaattg cgggtcgacg gagacgagat ccatattggc
     5101 ttcccaatag gcgtcgagcg taccgatgtc tctccagtag ttcacggcct tcttgttctc
     5161 gtcttgaaat ttgaaggcat agacgcggca ttccttgatc atccgcggaa tgatgttctt
     5221 gccgaaatcg tgtttggtgc cttccttggc atcgcgaatc agctgctctc ggacgacatc
     5281 cgtgcggaac agatagatgc ccatggagac gaaggcctgg gtcggatctc cgggaatgtg
     5341 catcggatgc tcgggcttct cgtcgaagct caagatgcgg cggtcttcat ccaccgcgat
     5401 cacgccgaac cggttggcat ccgcaatcgg ggtttcgatt gcgccgacaa tggcatccgc
     5461 ctgtttttcg atcaggaggt tgtacatatc ggcgtagttc atcttgtaga tatgatcgcc
     5521 ggccagcacc aggagaaact cggggtgatc gttatccatc aggaagagat tctgatagac
     5581 cgcatcggcg gtcccgcgat accactcttc gctgatccgc tgctgcggag gaaccgacac
     5641 gatgtattcg ccgagttcgg gattgagaat gttccatccc gtccggatat gccggtcgag
     5701 agaatgtgat ttgtactgga tcagcacggc gatctgccgg agacccgagt tcaggcagtt
     5761 gctcagtgtg aaatcgatga tgcgatattt accgccgaag gggaccgcag gttttgcgcg
     5821 ctggtcggtg agaggctgga gccgttcgcc tttccctccc gccagcacca tcgtgaagat
     5881 gttcgccata gatgcgccga ttgtagcagg acgaaggggg aatagaaagg acgcagaatg
     5941 attgacttca actccgactc ggataatact acgaatcaac atgcggttcc gacagcgggt
     6001 cgggccgaag agaaggagcg gcgatgaaga agcagaatgc tcgtccggcg atcatgaaac
     6061 cagcgatcga gccggccaat ccgatgatgg aaatggtgtg gcggctggag cgactggagc
     6121 ggcaagtgca gcgcctgtcc gagctcgtgc ggaatcacgc cgaggataac gaccggctcg
     6181 tgcgcatcgt ggcggaagat cgcgatgtga tccggcgtga attgttgcgg aaacatgagc
     6241 atcgggtgcg cgtgcggaag catctgcgcg acgtcagttc caaagtcgat ctggagcatt
     6301 gatccgagac cgtcggtgcg gcggtctgac cccagccctc gatggccacc ttttccactt
     6361 ctcctgtcag tgcctcaggc ccctctcttt cgtcgacagt cccgacgatc gacattcgag
     6421 ccatcgtgcg acatcatggc ccggtggcgg ccgtggatca cgtcagtttc caggtccggc
     6481 ggggagaatt tttttccatt ctcggcccga gcggggctgg aaaaacgtcg gtcttgcgga
     6541 tcgtggccgg tttcgaagat ccggatgcag gcgacgtgtt gatcgacggc cgatcgatgc
     6601 tgggagtccc gccgaaccgc cgtcccgtca acctggtgtt tcagtcctac gcactctttc
     6661 cccatctgac cgtctttgag aatgtcgcct tcggcctgcg gatgcgccgg gttccggctg
     6721 cgctcatcgc cgagcaggtt gagcaggcct tggcgatggt caagttggtc ggcaaggaga
     6781 ggcggaagcc tgcccaattg tccggcggcg agcagcaacg tgtggcgttg gctcgggcgt
     6841 tggtcaataa gccggctgtg gtcctgctgg atgaaccact cgcggccctc gatcaacaac
     6901 tgcggcaggc gatgcaggtg gagttgaagt cgattcagga acaggcaggg ctgacatgcg
     6961 tctgcgtgac gcaccatcag gaggaggcga tgatgatgtc cgaccgcatc gccgtcatgc
     7021 accaagggcg catgtggcag gtgggcactc cacgcgaagt gtatgagaac ccgcgcaacc
     7081 tgtttgtgtc tcgatttctc ggggtgtcca atgaactgcc ggggcgggtg gatgatgtcg
     7141 tggaagatgg gcatctcttt cacccggccg atgatcaggt gcccgcattt ccggtgcgcg
     7201 ctggttccga ggcgtcaccc ggtcattcag ccacctggtc gattcggccg gaacggctca
     7261 ggatgtcacg ggaacgacct gccggccgag acgcggtgct gaacggcttc atcgagaagg
     7321 tgctctttgc cggcagcgag acaaagtatc tcgttcgagt gggccgcgat aggctgtggg
     7381 aagcgaggac gaccggcgct ggcgaatcgc cccatgctcc gggggatccg gtctttctcc
     7441 actgggcctt gggtgacggg cgagtgtttg ttgagtgacc atgccgtctg acgatgtctc
     7501 ccacgtctct cccgcctcgc aagactcctg ccgctcttgc tggctcgccg ctcccggctt
     7561 gctgtggtta gccctgttct ttctcgttcc actggtgttg gtgtttgcca tcagctttgc
     7621 ctcgcgcgga acctatggcg gcatcgtctg ggagtttacg tgggcgaact atgttgatct
     7681 gctgcatccg ctctacggca aaatcctcgg ccagtccttg tggtacgcga cactgacgac
     7741 ggcgatctgt tttctgctgg gatttccttt ggcctatgtg attgcgcgca gtccggcacg
     7801 ctggcaaccg gtgttgctgc tcctggtcat gctgccgttc tggaccaact ttctcgtgcg
     7861 cacctacgcg tggatgatca tgttgcggca tgacggcctg gccaacaatc tgctgctcgg
     7921 tctcggactc attacggagc cgctggaatt gctctatacc ccgacggcgg tggtgattgg
     7981 attggtatat gggtacctcc cgttcatggt cttgccgctg tacgtggcgg tggagcgact
     8041 ggaccctgtc ctggtcgaag ccgcgtggga tctctatgca tcccgctggg cggtggtgac
     8101 gcgtgtggtg ataccgctca cgatgccggg catcgtgggc ggatgcatcc tggtattcat
     8161 tccgtcgatc ggctcgttca tcacgccgga tcttttgggt ggcgcgcgca gcatgatgat
     8221 cgggaacttg attcagcacg aatatctggt cgtgcgtgac tggccgctcg gttcggccgt
     8281 gtctttcgtt ctgatggcga tcgtgatggc ggcggtggtc ttgtactacc gtcaggccag
     8341 cccgggagac gggccattgg agggacggtg aagcagcgat cgctcggcct caatgccgtc
     8401 agcctcctgg cgatgctgtt tctttacggg ccgattatcg tacttgtgct gtactccttt
     8461 aacgccgcac acctctcaat gacctggcga ggggggaccc tgaaatggta tgcggcactg
     8521 gtccatgacg aagctttgct cgcagcgacg gcgaacagtc tgctcgtcgc cctggtgtcg
     8581 actatcggcg ccaccggtct cggagggctg ctggcgctcg gcatggaaca gatgccgcat
     8641 cgccggcaac agcttcttga aggcggtctc gtgcttccgc tggtcattcc cgaagtgatg
     8701 atgggcgtgg cgctgatgct gttgtttgtg acggttcgcg tgccgctcgg cctcaccacc
     8761 gtcatcctcg ggcacattgt gttcaatatc ccgctggtca ccatcatgat ccgcacgcga
     8821 ctgaggaaac tcgatcccat gttgcgcgaa gcggcggcgg atctcggcgc cgactcctgg
     8881 caggtattcc gccatgtgac cctgccctta ttgcggccgg cgatctgggg tgcggtgctg
     8941 gtcgccttca cggtctcgct cgatgatttt ctggtgacgt tcttcaccgc cggccccggc
     9001 tcgacgaccc tgccgctgaa ggtctattcg atgatcaagt ccggcgtgac tcctgaaatc
     9061 aatgcgctat ccgccttgct gttggtggtc tcgatgatag gtgtcgccgc ttccctgtac
     9121 ctgcagcgtc gcgaggtctg agtgagggca acgattcgcg agagggtcct gtggtgttta
     9181 tgcctggtgt ggctcaccgg gtatctcacg gggtgcgggc agcgcttcga tgagaaccgg
     9241 gtcgagactc gacctgtgtt gcactacttt acctggtcgg actacgtgag cccggacatc
     9301 attcaggaat tcgaacggcg cgagcaggtc aaagtggtga tcgatacttt cagcagcaat
     9361 gaagagttgc tggcgaaatt gcagagcggc gccacgggat acgacgtggc ggtcccttcg
     9421 gatttcatgg tggcgatcat gatccagcag ggcttgctca gtgagctgga tcaagccgcg
     9481 ttgtccaata catcccagct tgagccgcat ctccaaaccc tgcctttcga tcccgagcgc
     9541 cgctattccg ttccgtatct ctggggcacc gtggggatcg gatacgattc cgcgagcata
     9601 ccgacgcccc ccgacagttg gtcgatcctc tgggatgcgc ggtacaaggg ccgcatcagc
     9661 atgttgaacg accagcgcga agtgttcggg gcagtgttgc ggtcgatggg gcaatcgatg
     9721 aacagcacgg aaccgcaagt gattgagtcg gccaaagacc gcttgctcaa tcaaaagccg
     9781 ctggtgaaga cgtatacgag tgatcactac gaccagctgc tcgcctccgg ggaggttgtg
     9841 ctggcgcatg ggtggggagg gcaggtggcc cgtgccatgg ccgatcgccc ttccatccgt
     9901 tacgtcgtgc caaaggaagg cgccaccatt tgggctgatt gtctggtggt gcttcggacg
     9961 gcgccgcaga aggcactcgc catgcggttc atcaactatt tgatggagcc cactgtggcg
    10021 gcacgtacgt cggagcgact gcgttttgcc tccacctccc ggtcggcccg ggaacttgtg
    10081 gatgccgcga cgcgagacaa tcctgccgtc tatccttccc tcgatcaatt ggcacgattg
    10141 gagtggatgc gtgatgtggg caagggaatt cggctgtacg atcgtgcctg gaccgaactg
    10201 aagatgcaat gattgcggcc atgctcatta tttgaaatgc tgcgaagaat cagtataatg
    10261 cgtgccctgt cctcggttga tcgatattcc cgcaccatgc gtgacgacat gtctcagcac
    10321 aagaacggct ccccgagtgg tgtcaggtta tggcatcagc atcccgggct gtgggtcctg
    10381 attgcgctgc tgggtctgac cctattcggg agcggatggg
//
LOCUS       DAOQDS010000101        10217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_3309_length_10217_cov_17.480909, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000101 DAOQDS010000000
VERSION     DAOQDS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10217
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3309_length_10217_cov_17.480909"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(363..2795)
                     /locus_tag="PK589_17150"
     CDS             complement(363..2795)
                     /locus_tag="PK589_17150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ21393.1"
                     /translation="MMSSLRIFMVWCLGGWLGAFSVLVAHAQQPAGDASRTVVGIVQN
                     QDLRRVPQAAVQVKDQEGTVIVTAVANEAGEFTVDLPSQGTYSVSAQMDVDRSEYLIL
                     KSGAEPVGPVTLTLAKTNEISLEVVSPLAPIQYKASSETYALSRKEIESLPRGNNNDL
                     QDVLLTIPSAVYGSLKQVHIRQDHANLQLRIDGVPIPDTVSSTFSDVISPRAWDRADI
                     ILGGMEAQYGNKAAAVLDITTKSGTKPAFGSVQMMGGSNGTLNPSFEYGGTVGEKFRF
                     YILNSHTATNRGIEPPTLGHSVFHGQSERNQTFIRGDYQHDNKNNFTWLFLNSVAKYQ
                     IPTTPGLELDPSGQMLPLLQAGRPGFTPVASQAINEFQKENNQYGHMVWRHDVNANNF
                     FSLAGYMRHTRATFNTDPLNVLAYTADPTQPFSSADQDRNAYSTGLRFDYTYVHSKEH
                     LIKAGFQLDRTQSVNKTRLFTFADDGAGNPTGDLLSLNADNRQIGYRQEFWIQDQWSP
                     TDKWTFNLGLRGDAVQYLRNEGQVSPRLGATYKYDQSNVFHAFYGRMFTPPNLEAISF
                     AKLNTLGTRAQPEDVTNNTVRAERAHYFEVGSYHALNRYATLQFTGWYKLSNFLSDAG
                     QFGTTPLLNYFAFERGWQRGIDGALKLQLTDNLTARGNVAWGQCKGYGLQSGHFLLEA
                     KEIADINSRGGVFCDHMQTLTSSAVVTYKFLERTTMTGQMLFASGLRTAENEEAKTNS
                     THSPSYTIYNLSIAHVFPLPWDGQKFLLGFDIINLLDQKYYINQGEGSIGLGVAHAGM
                     PRSFFFRGQWFF"
     gene            complement(2890..3210)
                     /locus_tag="PK589_17155"
     CDS             complement(2890..3210)
                     /locus_tag="PK589_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21394.1"
                     /translation="MLPRYASVRFSSTIALLLVTLLLAMAPFAAALEIHHELAAADHD
                     GHEHSDTDLCQWVQYHTGHSLIGDVPVPCAFLAHAPPQLMPAPVLVSQSLPASRASRA
                     PPQR"
     gene            3391..4449
                     /locus_tag="PK589_17160"
     CDS             3391..4449
                     /locus_tag="PK589_17160"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNJ21395.1"
                     /translation="MNRPIDNQHVIEIKPLPSPRDVKTQLPISDEVSELVFQTRQAIR
                     NILHGRDMDRLIVVVGPCSIHDPDAAYEYADRLKPVAEAVRDKLLIVMRTYFEKPRTT
                     VGWKGLINDPHLDGTCDIAQGIQLARTILLNINGKGLPCATELLDPVTPQYIADLLSW
                     TAIGARTTESQIHREMASGLSMPVGFKNGTEGSLQVAINAMITSRSPHHFVGVNADGI
                     TSIIKTTGNPDHHIVLRGGGGRTNYSVEDIARAEAAVASEGLARGVMVDCSHDNSGKN
                     HQRQVEVAGDVLKQFQRGRRSIMGLMLESHLEGGRQNWDPNKTLTYGMSITDSCLAWS
                     DTEELLYGMAESLSAKTV"
     gene            4510..5289
                     /locus_tag="PK589_17165"
     CDS             4510..5289
                     /locus_tag="PK589_17165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNJ21396.1"
                     /translation="MLIDTHTHLDDARYASDRDAMIARAREAGVETMITIGCDLATSR
                     SAVALAGQYPFVYASIGVHPHEVKHITDSWYDELRQLAREHKVVAYGEIGLDYHYNHS
                     NPDEQRLRFREQIQLARELKLPVIIHTREAQDDTIRILKEERASEIGGVFHCFSGDAW
                     LAKDALELGFYLSFSGILTFQNATMLRDIAKTVPADRLLIETDCPYLTPIPHRGKRNE
                     PAYVKHVAETLATITSNGDTLTADDIGRLTSDNARRLFKIP"
     gene            complement(5276..5809)
                     /locus_tag="PK589_17170"
     CDS             complement(5276..5809)
                     /locus_tag="PK589_17170"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNJ21397.1"
                     /translation="MATHLLVDGYNLLGSARMAGPAGPGRLEAARDGLLHALAGYRQR
                     KGHAITVVFDGWQGGLGAEQREFQSGVEVVYSKRGERADQVIERLARLYGRDCAVVSS
                     DHEVINAARAAGAFVIGAPDFRAKLYQKPTRHQGPAFKELDRGEAEVVKRSKDKGGNP
                     RKLPKSQRKRNQQLREF"
     gene            5873..7231
                     /locus_tag="PK589_17175"
     CDS             5873..7231
                     /locus_tag="PK589_17175"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNJ21398.1"
                     /translation="MVPCREAFRLVPRMNWLFRLCLLLTLGWFLAGSASLSSAEMPSE
                     PSWPQIPQPSGDAAAPQFLLIKQDSAGTSRYPIIVRDLRTWSTPDGTRLVLDLNHKAS
                     FTETHVRNPDRVVIDISNAILGKSSRQRVSGGTIPQSFHITQSRPRVVTITLARTQGS
                     KYKAFALDNPDRLVIDIYHKAKDQIRQIGETSSTSPSAPAVALPTPPTIPSPTVTEPV
                     RPVAPKPASQIQTIVIDPGHGGKDPGTIGQHGTTEKDVTLKVGMLLKDLLSTLPNTRV
                     LMTREKDAFIELEDRAKFANGKSADLFVSIHVNSHPHKGVRGLEIYHFGEAKDQRALE
                     VAARENGTPLNSTGLGVEYILADLLTTKKIEHSQELAWTAKQAMVTNLNGRYSTIDHG
                     VKTAPFYVLRFTTMPSILAEIAFMSNPAEEEQMRTQPFLAHIAESIFEGVKTYLRTNP
                     SR"
     gene            7276..8040
                     /gene="truA"
                     /locus_tag="PK589_17180"
     CDS             7276..8040
                     /gene="truA"
                     /locus_tag="PK589_17180"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNJ21399.1"
                     /translation="MMTTFKLVLEYDGTRYSGWQRQLNASTVQAAVEAALAAISQTRL
                     TVVGAGRTDAGVHALGQVASFKTDRGLSPREWLRALNAHLPSDISALSVDAVPEHFHA
                     RYSATGKLYEYHILNRSERAALLRERAWSVYQPLNVAAMADAAAVLLGSHDFSSFETA
                     PTDNDNPHCRMQQSALRREGDLIVCSFYADRFLKQMVRSLVGTLVDVGRGKRSASDMD
                     TILKARSRPAAGRTAPPHGLYLVRVDYDAASAPPVT"
     gene            8122..8439
                     /locus_tag="PK589_17185"
     CDS             8122..8439
                     /locus_tag="PK589_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HNJ21400.1"
                     /translation="MKHLCAVLSSIVLAAVLVTPQLSYAGAQQNKMKTCNADADAKGL
                     SGEGKGEQRKAFMKECLSAKPEKAAAGASQQNKMKTCNKDAKAKNLAGDERKKFMKEC
                     LSN"
     gene            complement(8577..9269)
                     /gene="cysE"
                     /locus_tag="PK589_17190"
     CDS             complement(8577..9269)
                     /gene="cysE"
                     /locus_tag="PK589_17190"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNJ21401.1"
                     /translation="MSCKADDARTAIPAMLKAIAQDLQAIFDRDPAATSRLEVILTYA
                     GFHALLAYRLAHWLKQHQVPFLPRAVSQLARWLTGIEIHPSAKIGASFFIDHGMGVVI
                     GETAEVGDHVTLFQGVTLGGTGKERGKRHPTLGNHVVVGAGAKILGGITIGDNVKIGA
                     NSVVVKSVPPNSTVIGVPARIIKSQGERLPDATMDHTNMPDPTADRFAALEMELIELR
                     KKLENQDTGKPR"
     gene            complement(9266..9748)
                     /gene="ispF"
                     /locus_tag="PK589_17195"
     CDS             complement(9266..9748)
                     /gene="ispF"
                     /locus_tag="PK589_17195"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNJ21402.1"
                     /translation="MTGVRVGCGWDIHPLVAGRKLILGGLEIPHHQGLQGHSDSDALV
                     HAICDALLGAMGEGDLGRHYPSSDQRFKNISSLKLLEDVVGKLRAKGYRLANVDSTII
                     AQAPRLSPHLAAMQKSIAGVLQIDPDLVNVKVKSGEGLDAVGREEAIAAQAVCLIERD
                     "
     gene            complement(9745..>10217)
                     /locus_tag="PK589_17200"
     CDS             complement(9745..>10217)
                     /locus_tag="PK589_17200"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNJ21403.1"
                     /translation="SGGAQRQDSVRHALAEVSEADIILVHDAVRPFLTGDMIRRVVDA
                     AAEHGAAIIALPMRDTVKYVGAGGVIERTIDRGPLWLAQTPQAFRRACLEEAHRKALL
                     GNIHATDDAHLVELLGKPVVVVEGSGENIKVTRPEDLVIGEAILQSRTATRSAE"
BASE COUNT         2172 a         3190 c         2797 g         2058 t
ORIGIN      
        1 accatgggta ctgtcagtga tcgaattcca tcgatgatgt tcgtcatatc gtcttccaac
       61 cagtttcacg atgatgatgt tcacacgggc catccagcaa ggccacattt tctcacagtc
      121 tggaacagat gcccaacaag gttgtcagct aggccgcaca ccttcaacct tcctgtcgga
      181 aacggttgct caaacgggtc atccagcaag gccgcatttt cccacagtcc gaagcagatg
      241 cccaacaagg ttgtccagca aggccgcagg gaactcggcg actgaggcgt acccttgcgg
      301 tacgtcgcag ggagacgagt gactgagaac gctgctgctg gcgaccttgt tcggcatctg
      361 acctagaaga accattggcc acggaagaag aacgatctcg gcatccccgc atgagccaca
      421 cccagaccga tactgccctc tccctggttg atgtaatact tctgatccaa cagattgatg
      481 atgtcgaagc ccagcaaaaa tttctgtccg tcccacggca atggaaacac atgggcaatg
      541 gacaagttgt agatggtata cgagggactg tgcgtcgaat tggtctttgc ctcctcgttc
      601 tcggccgtcc gcaatcccga cgcgaacaac atctgcccgg tcatcgtcgt ccgttccagg
      661 aatttgtacg tcacgacggc ggaactggtc agcgtctgca tgtgatcgca gaacaccccg
      721 ccacgggaat tgatatcggc aatttccttc gcctccaaca ggaaatgccc cgactgcaag
      781 ccatagcctt tgcattggcc ccaggccacg ttgccgcgcg ccgtcaggtt gtcggtgagt
      841 tgcagcttca acgcgccgtc gatcccgcgc tgccagccgc gctcgaacgc gaaataattc
      901 aagagcggcg tcgtcccgaa ctgcccggca tccgacagga agttgctcaa cttgtaccag
      961 ccggtgaatt gcagcgtggc atagcgattc aacgcgtggt aactgccgac ttcaaaataa
     1021 tgcgcccgtt ctgcgcgcac cgtgttgttg gtcacatcct ccggttgcgc tctggtccca
     1081 agcgtattga gcttcgcaaa tgagatcgct tccagattcg gcggcgtgaa catccgtccg
     1141 taaaacgcgt ggaacacgtt cgactggtca tacttatagg tggccccgag ccgcgggctg
     1201 acctggccct cattccggag atactgcacc gcatctccac gcaggccgag gttgaaggtc
     1261 cacttatcgg tgggactcca ctgatcttga atccaaaatt cctgtcgata cccgatctga
     1321 cggttatcgg cgttcagact cagcaggtcg ccagtcggat tccccgcccc gtcatcggca
     1381 aaggtgaaga gcctggtctt gttcaccgac tgcgtgcgat cgagttggaa accggccttg
     1441 atgagatgct ccttactgtg tacgtaggtg taatcgaatc gcagcccggt cgagtaggcg
     1501 ttacggtcct gatcggcaga ggagaagggt tgagtcgggt ccgccgtata cgccaggaca
     1561 ttcaacggat cggtattaaa cgtggcccgc gtgtgccgca tgtagccggc gaggctgaag
     1621 aagttgttgg cgttcacgtc gtggcgccac accatgtggc cgtactggtt gttctccttc
     1681 tggaactcat tgatggcctg cgaggccacg ggcgtaaacc ccggccgccc tgcttgcagc
     1741 aatggcaaca tctgcccgct gggatccagc tccaagcccg gcgtggtcgg aatctggtac
     1801 ttcgcgacgg aattcaaaaa caaccaggtg aagttgttct tgttgtcatg ctggtaatcg
     1861 ccgcggatga acgtctgatt ccgttcgctc tggccatgaa acaccgaatg gcccaacgtc
     1921 ggcggctcga tgccccggtt cgtcgccgta tggctgttga gaatatagaa ccggaatttt
     1981 tcgccgactg ttccgccata ttcgaatgac ggattgagcg tgccattcga gccgcccatc
     2041 atctgcaccg acccgaaggc tggcttggtt ccgctctttg tcgtgatatc caataccgcc
     2101 gccgccttgt tgccgtactg cgcttccatc ccgcccagaa tgatgtcggc tcgatcccag
     2161 gcgcgaggac tgatgacatc tgaaaacgtc gatgagacgg tatccggtat cggaaccccg
     2221 tcgatacgca gctgcagatt cgcatggtcc tgccggatat gtacttgttt cagcgaccca
     2281 tagacggcgc tgggaatggt caacagcaca tcctggagat cgttgttatt gccacgcggg
     2341 agagattcaa tttctttccg gctgagcgca taggtttcgc tcgaggcctt gtactgaatc
     2401 ggcgccagcg gcgacaccac ctccagtgaa atttcattgg ttttggctag cgtcaacgtc
     2461 accggcccga ctggctcagc tccgcttttc agaatgagat attcgctccg atcgacatcc
     2521 atctgggcgc tgacggaata ggtgccctgg gagggcaggt ccaccgtaaa ctctcccgcc
     2581 tcattggcta ccgccgtcac gataacggtg ccctcctgat ccttcacctg cacagcggcc
     2641 tggggtacac gccggaggtc ctggttctgc acgatcccga cgaccgtccg gcttgcgtct
     2701 cccgccggct gctgggcgtg agcgacaagg acgctgaaag ccccgagcca acctccgaga
     2761 caccacacca tgaatattct caatgacgac atcatgactc cctcacgaaa attccccgcc
     2821 tgcgcggagc gaggcacagc ctcggtgctc aacgcaggtc accagtgatg cacatatggt
     2881 ggcgcagaat tatcgttgcg gaggtgcgcg agaagccctg gatgcgggaa gggactggga
     2941 gacgagtaca ggcgccggca tgagctgcgg cggcgcatgc gccaggaacg cgcaggggac
     3001 cggcacatca ccgatcagtg aatgaccggt atgatactgg acccactggc agaggtcggt
     3061 atcggaatgc tcgtggccgt catgatcggc ggcggccagt tcgtgatgga tctccaacgc
     3121 ggcggcgaac ggcgccatcg ccagcaggag agtcaccaga agcagggcga tggtcgaact
     3181 gaatctcacg gaggcgtaac ggggcagcat agccatctca tcgtctaagg acacgtcggc
     3241 aaacttggat tttgtagcaa agcgcccggt tgaaagcaag gcaatgcgcc gatcctgccc
     3301 accctctgga ctttctcttt cacttttcgc tatgttaact cacaattttc atgtcacacc
     3361 ttagcagaga gaccgttcaa agggaccatc atgaataggc cgatcgataa ccagcatgtc
     3421 atagagatta aacccctccc ctcgccccgc gatgtcaaaa cgcagctgcc tatcagcgac
     3481 gaggtatccg agctggtctt ccagacgcga caggcgatcc ggaatatcct gcacggacgt
     3541 gacatggacc gcctcattgt ggtggtcggc ccctgctcca ttcatgatcc tgatgccgcc
     3601 tacgaatacg ctgaccggct caaacccgtc gctgaggccg ttcgcgacaa actgctgatc
     3661 gtgatgcgca cctatttcga gaagccacga acgactgtgg gttggaaggg tctcatcaac
     3721 gaccctcacc ttgacggcac ctgcgacatt gctcagggca ttcagctggc ccggacgatt
     3781 ttgctgaaca tcaatggcaa gggccttccc tgcgccaccg aactgcttga tcccgtcacg
     3841 ccgcaataca ttgccgacct cctgagctgg accgccatcg gagctcggac aactgagagc
     3901 cagatccacc gtgaaatggc cagcggcctg tcgatgccgg tcggattcaa gaacggcacg
     3961 gagggcagcc tgcaggtcgc catcaacgcc atgatcacca gccggagtcc ccatcatttt
     4021 gtcggggtga acgccgacgg gatcacctca atcatcaaaa ccaccgggaa tcccgatcat
     4081 cacatcgtct tgcgcggcgg cggcggacgc acgaactaca gcgtcgagga catcgctcgc
     4141 gccgaagcgg ccgtggcaag cgaagggttg gcccgcggcg taatggtgga ctgttcgcac
     4201 gacaactccg ggaagaacca tcaacgccaa gttgaagtgg ccggcgacgt cttgaaacag
     4261 ttccaacggg gccggcgctc catcatgggt ctcatgctgg aaagtcacct ggaaggcggc
     4321 cggcagaact gggatccgaa caaaactttg acgtacggca tgtcgattac cgattcctgt
     4381 ctcgcctgga gcgacaccga agagttgctc tacggtatgg ctgaatccct ctcagccaag
     4441 acagtctagg ctcacatcgc cctgtcactt cgatttccac tgtcacagcc ggatgccgga
     4501 atccactcga tgttgatcga tacccatacc catctcgacg atgcgcggta cgcctccgac
     4561 cgcgacgcga tgattgccag agcaagggaa gcgggtgtgg agacgatgat cacgatcggc
     4621 tgtgatctcg cgacaagccg atcggcggtt gccctcgccg gtcagtaccc tttcgtgtat
     4681 gcctcgatcg gcgtccatcc gcacgaggtc aaacacatca cggacagctg gtatgacgaa
     4741 ctgcggcaat tggcgcgaga gcacaaggtc gtggcctatg gcgagatcgg cctcgattac
     4801 cactacaacc actccaatcc ggatgagcaa cgcctccgat tccgtgaaca gattcaactc
     4861 gcccgggaat tgaagctccc cgtcatcatc cacactcggg aagcgcagga cgataccatt
     4921 cgcatcttga aggaagagcg ggcgtcagag atcggcgggg tatttcactg cttttccgga
     4981 gacgcctggc tggcgaagga tgcgctggag ttgggattct acctgtcatt ttccggcatt
     5041 ctgacgtttc agaatgcgac catgctgcgg gacattgcca agaccgtccc ggctgaccgg
     5101 ctgctcatcg aaaccgattg tccctacctc accccgattc cccatcgagg caaacgcaac
     5161 gagccggctt acgtcaaaca cgtcgccgaa acgttggcca cgatcacgtc caatggcgac
     5221 acgctgacag cggatgacat cggccgcctc accagcgaca atgcacgacg cctgttcaaa
     5281 attccctgag ttgctggttt cgtttacgct gggacttggg gagcttgcgg ggattcccgc
     5341 ccttgtcctt tgatcgtttc acgacctcag cctcgccccg atccaattct ttgaatgccg
     5401 gaccctggtg gcgcgtcggc ttctgataca gcttcgcgcg gaagtccggc gcaccgatca
     5461 cgaaggcccc ggcggccctt gccgcattga tcacttcatg atccgatgac accaccgcgc
     5521 aatccctgcc atacaatcgt gccagacgct cgatcacctg gtcggcgcgt tcgccccgct
     5581 tggaatagac cacctcgacg cctgattgaa actcgcgctg ttcggcgccc agtcctccct
     5641 gccatccatc gaacaccacc gtgatcgcat gccccttgcg ctgacggtag ccggccaggg
     5701 catggagcag gccgtcgcgc gccgcttcga ggcggccggg accggctgga cccgccatgc
     5761 gcgcgcttcc caacaggtta tatccatcga caagaagatg tgtcgccatc tacaattacg
     5821 cagtttgaga ccttttgttg acaataccat aaacatctga gaaaaggcct agatggtacc
     5881 ttgtcgagaa gcgttccgac tcgttcctcg gatgaattgg ctgttccgtc tttgcctcct
     5941 gctgaccctc ggctggttcc tggctggttc ggcttccctg tccagcgcgg agatgccgtc
     6001 cgagccatcc tggccccaga ttccccagcc gtccggggac gcagcggcac ctcaattcct
     6061 gctgatcaaa caagattccg ccggcacctc gcggtatccg atcatcgtcc gcgacctgcg
     6121 cacctggtcc actccggacg gcacacgcct ggtgctcgac ctgaatcaca aagcctcctt
     6181 caccgaaacc cacgtgcgaa atcccgatcg ggtcgtcata gacatcagta atgcgattct
     6241 gggaaagtcc tcccggcagc gtgtctccgg cggcacgatt ccgcaatcgt ttcacattac
     6301 gcaaagtcgt ccgcgggtcg tcaccatcac cctcgcgcgc acccagggct ccaagtacaa
     6361 agcgtttgcc ctggacaatc cggaccgcct cgtcatcgac atctaccaca aggccaaaga
     6421 tcagatccgg cagatcggcg agacgtcctc gacgtcgcca tcagcccctg ccgtggccct
     6481 cccgactccg ccgacgattc ccagccccac ggtgacggaa ccggttcgcc ccgtggcgcc
     6541 taaacctgcc agtcagattc agacgatcgt gatcgatccc ggccatgggg gaaaagaccc
     6601 cggcaccatc ggacaacatg ggacgacgga aaaagatgtc acactcaaag tcggcatgtt
     6661 gttgaaggac ctcttgagta ccttgcccaa tacacgcgtg ctcatgactc gcgagaagga
     6721 tgcgttcatc gagttggagg atcgggccaa gtttgccaac ggcaaaagcg ccgatctgtt
     6781 tgtatccatc cacgtcaact cgcacccgca taagggggtg cgcggcttgg aaatctacca
     6841 cttcggcgaa gcgaaggatc agcgggcgct ggaagtcgcg gcgcgggaaa acggcacgcc
     6901 gttaaacagc actggactcg gcgtggagta catccttgca gatcttctga cgaccaagaa
     6961 aatcgagcat tcacaagaac tggcctggac ggccaaacag gcgatggtca ccaatctcaa
     7021 tggtcggtat agcaccatcg atcatggcgt gaagaccgcg ccgttttatg tcctgcggtt
     7081 tacgaccatg cccagcattc tcgcggaaat cgccttcatg tcgaatccgg cggaagagga
     7141 acagatgcgc acgcaaccgt tcctggcgca tatcgcggaa tcgatcttcg aaggggtcaa
     7201 gacatacctc cgcaccaatc cctcccgatg acagccgcct tcccgttaag ctagagtgct
     7261 gcttttcgca ctcacatgat gaccacgttc aagctcgttc tcgagtacga tggaacccgt
     7321 tactcaggct ggcaacgcca actgaatgca tcgaccgttc aggctgcagt ggaagcggcg
     7381 cttgcggcga tctcgcaaac gaggcttacc gtggttgggg caggtcgcac ggatgccggc
     7441 gtgcatgccc tcgggcaggt ggcgagcttc aaaaccgatc gcggcctttc gccacgagag
     7501 tggcttcgcg ccttgaatgc ccatctcccc tcagacatca gcgcgttgtc ggtcgatgcc
     7561 gtgccggagc attttcacgc ccgatattcg gccaccggca aactctacga gtaccacatc
     7621 ctgaatcgat cggagcgggc ggctctgctg cgggaacgag cctggtctgt ttaccaacca
     7681 ctcaatgtcg ccgcgatggc cgatgccgca gccgtcctgc tcggctcgca cgatttctcc
     7741 tcgtttgaaa ccgcgcccac cgacaatgac aatccgcact gtcgcatgca gcaatcggca
     7801 ctccgccggg agggcgatct catcgtgtgc tccttctatg ccgaccgttt cctcaaacag
     7861 atggtgcgca gtctcgtggg gacgctggtg gacgtaggac gcggcaaacg atcggcgtcc
     7921 gatatggaca caattctcaa ggcccgctcc cgccctgcag ccgggaggac ggccccgccc
     7981 catgggttgt atctcgtcag agtggattat gacgcggcat ccgctcctcc tgtcacctag
     8041 gtaactgtcc cgtgagacag aaccggcaat ttttgctata gttccgccga catcccccgt
     8101 ccacctcaac aggagacttc catgaaacat ctttgcgcag tcttgtcctc gatcgtgttg
     8161 gcagccgtgc tcgtcacgcc gcaactcagc tatgccggag cacaacagaa caaaatgaag
     8221 acctgcaatg ccgatgcgga tgccaagggg ctcagcgggg aaggcaaagg cgagcagcgc
     8281 aaggcattca tgaaagaatg tctctccgcc aagccggaaa aagccgcagc cggggcgtct
     8341 cagcagaaca agatgaagac ctgtaataag gatgccaagg caaagaatct ggccggcgat
     8401 gaacggaaga agtttatgaa ggaatgcctg tccaactaac cgcagcgacc ccggcatggt
     8461 actaggctgt gccggggttc atcgatcacc cacacaacat cccgtcttcg ccgaagcagt
     8521 cggcgtggta cctgtctcca atcgcacatc aatagaggaa cgagcgtcgc ggcgggttag
     8581 cggggcttac cggtgtcctg gttttcgagt ttctttcgta actcgatgag ttccatctcc
     8641 agcgcggcga agcggtctgc tgtaggatcc ggcatattgg tatgatccat cgtggcgtcg
     8701 ggaagccgct cgccttgtga tttgatgatc ctggccggca ctccaatgac ggtcgagttc
     8761 ggcggaaccg atttgacgac gaccgaattg gcgccgattt tcacattgtc gccgatggtg
     8821 atgccgccga gaatctttgc acccgccccg accaccacat gattccccag cgtgggatgg
     8881 cgcttgcccc gctctttgcc tgtgccgccc agcgtcacgc cctgaaacaa ggtgacatgg
     8941 tcgccgactt ccgcagtttc accgatcacc acacccatgc cgtgatcgat aaagaaggag
     9001 gcaccgattt tggcagaggg atggatttcg attccggtca gccaacgcgc cagttgtgac
     9061 acggcgcgcg gtaagaacgg cacttgatgc tgtttcaacc aatgcgcaag ccggtaggcc
     9121 agcaatgcgt ggaatcccgc ataggtcaaa atgacctcga gcctgctcgt cgcggcagga
     9181 tcccggtcga aaatcgcctg gaggtcttga gcaatggcct ttaacatagc tgggatagcc
     9241 gtacgcgcgt catccgcctt acaactcagt cacgctcgat cagacagacg gcttgcgcgg
     9301 cgatcgcttc ttcgcgaccc accgcatcca gcccttcacc actcttcact ttcacattca
     9361 ccagatcggg atcgatttgc agcaccccgg caatcgactt ttgcatcgcg gccaaatgcg
     9421 ggctcaagcg aggcgcctgg gcaatgatgg tgctatcgac attcgcaaga cgatagcctt
     9481 ttgcccgcaa tttgcccacg acgtcttcga gcaacttcaa gctggagata ttcttgaacc
     9541 gctgatccga gctcggatag tggcggccca gatctccttc tcccatcgcc cccaacaggg
     9601 catcgcagat cgcatgcacg agcgcatcgg aatcggaatg tccctgcagc ccctggtgat
     9661 gcggaatttc gagtccgccc aggatcagct tgcgtccggc caccaaggga tgaatatccc
     9721 acccacaccc cacgcgtaca cccgtcattc tgcactcctc gttgctgttc gtgattggag
     9781 aatcgcctcg ccgatcacga gatcttccgg ccttgtcacc ttgatatttt caccgcttcc
     9841 ctcgaccacc acgaccggct tccccaacaa ctcgacgaga tgcgcgtcat ccgtcgcatg
     9901 gatgttgccg agcagcgcct tccgatgcgc ctcctcaagg caggcacgcc ggaaggcctg
     9961 cggggtttgc gcaagccaca agggaccacg atcgatggtt cgctcgatca cgccgccggc
    10021 cccgacatat ttcaccgtat cccgcatcgg aagtgcaatg atggccgctc catgctccgc
    10081 cgctgcgtca accacacggc gtatcatgtc cccggtcaga aacggccgca ccgcatcgtg
    10141 cacgagaata atatccgcct cactcacctc ggccaacgca tgacgaaccg aatcctgccg
    10201 ttgcgctccg ccggaca
//
LOCUS       DAOQDS010000102         9581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_3701_length_9581_cov_25.907411, whole genome shotgun sequence.
ACCESSION   DAOQDS010000102 DAOQDS010000000
VERSION     DAOQDS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3701_length_9581_cov_25.907411"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(451..831)
                     /locus_tag="PK589_17205"
     CDS             complement(451..831)
                     /locus_tag="PK589_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNJ21404.1"
                     /translation="MATGTVHNHIFFTNILRLLDERGMTKNELAAKADISVSFLSDLT
                     NGKANPSLKIMEAIAEALETPLPTLLESTDLDRESLDALAGGNAPRSLPEGVVRVSAV
                     LPEHQAFIVKKWDEAARNKLRKPS"
     gene            complement(1235..2239)
                     /locus_tag="PK589_17210"
     CDS             complement(1235..2239)
                     /locus_tag="PK589_17210"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNJ21405.1"
                     /translation="MKTTIRTARLKAGLAVNRELVLLYWRIGKEILERQSAQGWGAKV
                     IDQLARDLRAEFSDMRGFSRTNLLYMRAFASAWPEEAIVHQFGGQIPWRHNCVIIDQV
                     KDRPTREGYIRKTIENGWSRSVLELQIETAAHRRAGAAQTNFDRALPWPQSDLARDIL
                     KDPYTFDFLGITDASNERTIEKALVLRLRDFLIELGVGFAFVGSQYRLEVEGDEFFMD
                     LLFYHTRLHCYVVIELKNTAFRPEYVGKLNFYLAAVDDLVRNQTVDAPTIGLVLCKSK
                     KGTVVEYALRDINTPMGVSTYRTALPDAMAGVLPSIEALTEELQTLQGGEQDDVEPDH
                     "
     gene            3210..3467
                     /locus_tag="PK589_17215"
     CDS             3210..3467
                     /locus_tag="PK589_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21406.1"
                     /translation="MIYACTNDTDLDELIGTQCWEGQRLSFHYGPLVQAMKAGEELVL
                     ENSAALSAFMLAKVRLMLGAMTIEDTSEEIYPHDGFRLTLG"
     gene            complement(4266..5795)
                     /locus_tag="PK589_17220"
     CDS             complement(4266..5795)
                     /locus_tag="PK589_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21407.1"
                     /translation="MNLEQVLEVAAYARPGYSLVSFKESAIPNYLLTTRLLTLEKKSL
                     GPIEEACLGAVSAGLGDPDDLSSFLGLPRVVLNGILAGLNTTECINYSRPTENSDAIV
                     SLTEKGRLTLTTLKEVVPQETVVKFVFDPYLRRIRFVSTASLFKPREVKEQGWYEVPL
                     CGAKRPEVDDVPLADFDKVLQKLPRRDDNTRELLAARRIERREMHFVPCVLLFYRAQS
                     GQEVQVAFYLDDGFSLEHENAFRELGGPELAGANHALTAPEMPVLEDLIEGLNEAQKV
                     SELLSLEQEVSNTMQSEASLDASRIEQIATEGDSERTPSSGVPNAVVARLRAMTQRCI
                     RTHEHPGLLRKALTGSQNRLLIVSPWITHWVIDNMFVMSLEALLRNGVAVTIGYGLAD
                     DEGARGTDKAKQKPAITLQAEKELASIKKRFDNFKLVFLGNTHRKLLVSDDKFAVVTS
                     FNWLSFRGDPGRKARDEFGFLVTEPDDVESIFHDAIELLEKGYDGLAAGRTAEAGKGK
                     R"
     gene            complement(5792..9280)
                     /locus_tag="PK589_17225"
     CDS             complement(5792..9280)
                     /locus_tag="PK589_17225"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNJ21408.1"
                     /translation="MNPINGTLTLHGVKKKSDIKKRFLADGRYLISGEGRSGGIASVF
                     RVFDTQEEKHVALKVFRPVNGADPVVEESFRRETLALSDLKHPNIVQILDSGFDEDTN
                     EHFIAMEWIEDVLESFLGRTRLSDWGVFFAKIGRPILEALAFAHSHRTVHRDIKPSNI
                     LLTSEGVPKVCDFGISKIRNFLEPGVTLAHFASFPFAPPEMDDGSYSYSRDVFGFVAL
                     SVAAITNERIVNHNALIAGLEKLKIDEPLKRLLRRSLSLDNPAERQVNAAVLLAEIDR
                     LVPPPPTQKVGRILIGLTNKVKQIIDYDLALKGDPEIQNFVEADLQSAFCIHDPAEPR
                     PGPDGSAPLGRLIRLLGGRYGYVALMAPPAGEKLLLISALEQSASESERNREAASPSR
                     YEFSFGGVLPITSAGNIVKLQDELLQFGADQKADRFQQRQKAIYKTWLDLLSAKTELE
                     RQRKRRCPYRDIEISGSVIRFLVANGADTRTLDGQDIQADSESGETFTGTVISVSDVS
                     VVVQPGERNRVEPSSLHKQGAIVVDTVKADVALDRQKTAVEAVRFGRSINPLLGNYIV
                     DPSSVPVPVTREVSFIQQAMDDDKREAVRTALSEPALMLVKGPPGAGKTTFITEVVLQ
                     TLRSNPNARILLTSQTHVALDNSLERILKESNEEVRAIRIGNESDERITEKAKTLLID
                     RKLPIMRKAALAKGREFVESWAQKSGVDLTSTRMAMALDRHAGLRERLERIQSQIAEF
                     DKTLSRDKNSLEGEVRADMEELQAELVKEQDALTRDLKESLAELRKHETDKETLEHLA
                     DCSAEELRSWADSYVKRSTQVIQLRKMLAVHSEWEVRFGRSPQFKAALVTQAQVVAGT
                     CLGVMGIPGRNEITYDLCIIDEASIATPTEVLVPMARARRTILVGDDRQLSPFQDPEL
                     QTSGILQRFSLTAANQKETLFNHLRDGLPEGLRKVLATQHRMIPAIGNLISACFYGSE
                     LKSADKLPCGLLPRTLQKPVTWFSTSRIDERASKQVGTSQWNGLEVKYISDLIGRIDF
                     ELRHGKAKGTKVSIAVLTGYGEQRARLHQAIQNRRHDWQSFSDIYVNVVDAFQGREAD
                     ILIFSVTRSDVRGLGFLREMERINVALSRGKEYLVIVGDHQFCQEAEGRSNPLKGVID
                     YIRGNPQDCILEEIKP"
BASE COUNT         1994 a         2782 c         2657 g         2148 t
ORIGIN      
        1 tgccgtcggc attgagcatc atctcgacgg tcctggggtc agccagggcg tccatgatga
       61 gcggccccag atcacgctcc agctttctct tcgcccgatc cttgagggaa gaaacggatt
      121 cttcgatgtc tgtcatgtat cgatcccggt ttgcttcggt tgtatgtcaa ccttcgacct
      181 gctggatacg atataacgaa tttcagaact ttgcaagaca acgactatgg tgaaccataa
      241 ggtggcggtg tgcaagaccc agaccaagtc gatggagcga gcagcgtgta ctggcgtcgg
      301 agaggcggcc cttggtgaca agctggggcg ctcgtcaggc gtggactgtg taatccacag
      361 ctgggcgttt tcggcgcgga atgcgccttg gcgggagagg agagggggag ggcagggagg
      421 gcttcagccg ctaaaggccc tcactgggcg tcaggacggt ttgcgcagct tgttccgtgc
      481 cgcttcgtcc cacttcttga cgatgaacgc ctgatgttcc ggcaggactg ccgacacccg
      541 caccacgcct tcgggaaggc ttcgcggggc gttcccgccg gcaagggcat ccaggctctc
      601 gcggtcaagg tcggtcgatt ccaggagcgt tggcagcggc gtttccaggg cttccgcaat
      661 tgcctccatg atcttcaggg aagggttcgc cttgccattg gtcaggtcgg acaggaaaga
      721 gacggagatg tcggcctttg cggccagttc gttcttcgtc atgccgcgct cgtcgagcaa
      781 gcgaaggatg tttgtgaaaa agatgtggtt gtgcacagtg cccgtcgcca tccggttgtg
      841 cggctcttct aaatttagcc gctaaagttc ttgatcttga attttgtttt agctaaagtc
      901 cggcttgatg ccatccggag aagcgggtct acagacgacg caggcccatc gttcggcaat
      961 cctgcccgtc gacgtggcca gccgacagcc gttaatgatc ggcgtcgtat gcaccttcac
     1021 gatcccccgg caaatcgaaa gcaagaaaca cgccgtatta cccgtgccgc ataaaatcaa
     1081 tggcttggtt ctggtttgtg ggaaacggaa gggcgaactg taaaaatagt cgacagtcct
     1141 cggcaaccct ctggccgtgc ttgggcgtga ccagtgctcc cgggtatttt gggttgcaag
     1201 cggaaggcat atccaccgcg gagtgtgcat tgcttcagtg atcgggttcg acatcgtcct
     1261 gttcgccgcc ctggagcgtt tgaagttcct cagtcagcgc ctcgatgctc ggcaggacac
     1321 ccgccatggc gtccggcagg gcagtgcggt aggtggagac ccccatcggc gtattgatgt
     1381 cacgcagcgc atactcgacg acggttccct tcttgctctt gcagagcacg agaccgatcg
     1441 tcggcgcatc gacagtctgg ttgcgcacca ggtcatcgac ggcggcgagg tagaaattga
     1501 gcttgccgac gtactcgggc cggaaggcgg tattcttgag ctcgatcacc acgtagcagt
     1561 gcaggcgggt gtggtagaag agcaggtcca tgaagaactc gtcgccctcc acctcaagac
     1621 ggtactgact tcccacaaac gcgaaaccga cgccgagttc gatcaggaaa tctctcaggc
     1681 gcagcacgag cgccttttcg atggtgcgct cgtttgacgc atccgtgatc ccgaggaagt
     1741 cgaaggtgta ggggtccttg aggatgtcgc gcgccaggtc ggactgtggc cacggcagcg
     1801 cacgatcgaa gttggtttgc gcggcacccg cgcgccggtg agcggccgtc tcgatctgga
     1861 gttcgagaac cgaccggctc cagccgtttt cgatggtctt gcggatatac ccctcgcggg
     1921 tcggccggtc cttgacctgg tcgatgatga cgcagttgtg ccgccatggg atttgtccac
     1981 caaactggtg gacaattgct tcctccggcc acgctgaggc aaatgcccgc atgtagagca
     2041 gattggtgcg ggaaaaacct ctcatgtcgg agaactcggc acggaggtcg cgggccagtt
     2101 ggtcgatgac cttcgccccc cagccctggg cggattgccg ttcgagaatc tccttgccga
     2161 tgcgccaata gagcaggacc agttcccggt taacggcgag gccggccttg agtctcgccg
     2221 tgcggatggt ggtcttcaac tccgcaagcc atgcggcgta gtcgggaggg ataagggttg
     2281 tcacgcttgg ggtgtcatgg tttgcggcga gtcacttgat ctggaattgt ccaccaggct
     2341 gctgcactgt tgcgcgacgg tagctcgttg ggcggtacgc tcagcctttc ggtgactcat
     2401 gcggaaatcc ctacaccccc aaaagtctga tgctgacgac gatcggatat gtattcggaa
     2461 ttgtacagcg ggaagattct ctgcagacga tttgtggctc gccagatcaa gcgcgtttgc
     2521 ggagtcaagc gtcaatcgac ccggcacgcc tcccagggtc ggcccgccgg catggaacga
     2581 agcgcgaaga acggcttcgc catacatcgc gccgaaccca ccgctaggga atagatgggc
     2641 catcctggcc agatcggagg ccgtgtctac cagaccagag aggattcggc accggcaacg
     2701 ccagtgctgc gcactcttgt ccaacctgcg caaatctgca atcgaccatg gagcaagaaa
     2761 gggcagtaac gtgagcaggt ttcacgcgat catcctcagg tcagcgatgg cacgttgcag
     2821 gcctcgaacc acgtcaaccc acgccttgtc gtggctactc caagtgtttc aagaaaatgt
     2881 atctgatgac attcccacct gacgggttcg cccaaaccac caaagactcc ctcaactcgc
     2941 acgatgcgca atcgcttcaa gaattcttaa agcagtctga cgccagcccg gtgtcgcggg
     3001 taccaccgtc tccgccagcc tgatcgcttc cagatcgtca ggcacgttcc attctcggtt
     3061 gtccattcgc agaaggatgc tccgggcgag gcagtccacc agagagtctt gattcggtca
     3121 attggacctc aatttcagat tccgactcaa tttcttcctg cgatcgtccg ttaatcaatg
     3181 caagaccttc tgctctctgg aggaataacg tgatctacgc gtgcaccaac gatactgatc
     3241 tcgatgaact catcgggact caatgctggg aaggccagcg gctgtcgttc cattacggcc
     3301 cgctcgttca ggccatgaaa gcgggcgaag agttggtcct ggagaacagt gccgcgttgt
     3361 cggctttcat gctcgcgaag gttcgcctga tgctcggcgc catgactatt gaggacacct
     3421 cagaagagat ctacccgcac gatggttttc ggctgacgct cgggtaaggt cggctggatc
     3481 tgctgttcga gcaagccacc ggtctcgaga agcgctactt gcaggcgctg gatgcccgcg
     3541 aggatccaga cgccgaaaga agccgggagc ctgctctccg gttacgggtt tgccggtgcg
     3601 gcgatgcaat tgtccgcgat tggtatggta tcgacggcac cggcaacggg ttaaacagcg
     3661 aggagtcgcc cccacggcaa ggatccagcg gtcgtgccga cgccgacgag cttgaattgt
     3721 catgctccgt ggaagacttg ctccaacacg catcgggacg ggacgacgca ctggcaaccg
     3781 aggttgagag cgagatcaac cagtatcgtc accaggcgca ctgcatcgcg gagatgctgg
     3841 cgacgctggg gcgtctgcaa cgccttgtac actccccgcg ccggacaagc ggtggagagc
     3901 agcgtgcttc ccgtctcacc gcttgcgagg ccatcggcgt cgcacccgct gccgcgatcg
     3961 cttcactgga agcagcagga gcaaggtctt gacgtttagg tccgcttccg accctcagcg
     4021 gtcctacgcg gtatcccgac ttctacggct ggtgacagag cagaccggcc gttcatggcg
     4081 actgctcacc gggaattcgt cggccaaagc cgacgtacag cacacgccag atgagaggct
     4141 ggtcttccga ggtcaacggg ccttcagatc aacgagatcg cgaacgtctg ctcttctgac
     4201 ggccgaacgc gaagtctcga ccccttgcca ctggtactcc taattcttaa tcacagtgcc
     4261 aaacgtcacc gtttgccctt tcctgcttct gctgttcttc ctgcagccaa gccgtcgtag
     4321 cctttttcca gcagttctat cgcgtcatgg aaaatgcttt ccacgtcatc cggctctgtc
     4381 accagaaatc cgaactcgtc ccgcgccttt ctccctgggt cgccacgaaa cgacagccag
     4441 ttgaagctgg tcacgaccgc aaacttatca tctgaaacca gaagttttcg gtgcgtattc
     4501 ccaagaaaca ccagtttgaa attgtcgaat cgctttttaa tgctcgcaag ttccttttct
     4561 gcctgtaagg tgattgcggg cttctgcttg gctttgtccg tgccccgcgc accttcgtcg
     4621 tcggccagac cataccctat agttaccgcc acgccattcc ttaacagggc ttcaagtgac
     4681 atcacaaaca tgttgtcgat gacccagtga gttatccatg gagaaacgat aagaaggcgg
     4741 ttctgcgaac ccgtcagggc ctttcgcaac agccccggat gctcgtgcgt acgaatgcat
     4801 cgctgagtca tcgctctcag gcgggcgaca actgcgttgg gcacgccact ggaaggcgtc
     4861 ctctcactgt cgccctcggt tgcaatctgt tctatacgcg aggcgtcaag tgaagcctca
     4921 gactgcatcg tgttcgatac ctcttgctca agcgagagca gttcgctgac cttctgtgcc
     4981 tcattcaatc cttcaatcaa atcctccagt actggcattt ccggcgccgt cagcgcatga
     5041 tttgcgcccg ccagttcggg accaccaagt tcccggaacg cgttctcatg ctccagggag
     5101 aacccgtcgt ccaaatagaa ggctacctga acctcctgac ccgactgagc ccggtagaac
     5161 agcagaacgc atggaacgaa gtgcatctcc ctgcgctcga tcctcctcgc ggccagcagc
     5221 tccctcgtgt tgtcgtctcg gcgcggcagc ttctgcaaga ccttgtcaaa atcggcaagt
     5281 ggcacgtcat caacttcagg gcgcttcgca ccacacagag gcacctcgta ccatccctgc
     5341 tccttgacct ctctcggctt gaacagggac gccgtcgaaa caaaacggat tcttcgaaga
     5401 tagggatcga atacaaactt gacgacggtt tcctgcggga caacctcctt cagggtcgtc
     5461 agcgtaagcc gtcccttttc ggtcagcgag acaattgcat ccgagttttc cgtcggacgc
     5521 gaatagttga tgcactccgt ggtgttgagc ccagcgagta tcccgttcag taccacccga
     5581 ggaaggccaa ggaacgacga aaggtcgtcc gggtcgccaa ggccggcgct tactgccccc
     5641 agacaggctt cttcaattgg accgaggctt ttcttttcaa gcgtaagtag ccgcgtggtc
     5701 agcaggtagt tcggaatcgc ggattccttg aacgacacca gcgagtagcc ggggcgggcg
     5761 tacgcggcaa cctcaaggac ctgttcgaga ttcatggctt gatctcctca agaatgcaat
     5821 cctgagggtt gccccggatg tagtcgatta cccccttcag cggattggac cggccctccg
     5881 cttcctgaca gaactgatgg tcaccgacga tgaccaggta ttccttgcca cgcgataacg
     5941 caacgttgat tcgctccatc tcgcgcaaga atccaaggcc gcgaacgtca gatcgggtga
     6001 cggaaaagat caggatatcg gcttcccttc cctgaaatgc atcgaccaca ttcacgtaga
     6061 tgtcagaaaa ggactgccag tcatgccttc tgttttgtat cgcctggtgc aatcgggcgc
     6121 gttgctcacc gtagcctgtc aatacggcaa tcgatacctt tgtacctttg gcttttccat
     6181 ggcgaagctc aaagtcgatc ctgccgataa ggtcggaaat gtatttgact tccaacccgt
     6241 tccattgcga cgtaccgacc tgtttcgatg ccctttcgtc aattctgctc gttgaaaacc
     6301 aggtcaccgg tttctgcaag gttctcggca aaagcccgca aggaagcttg tctgcgcttt
     6361 tcagctccga accatagaaa caggctgata tcaggtttcc tatcgcagga atcattcgat
     6421 gctgggtggc gagtacctta cggagccctt ccggaagtcc atctcgaaga tggttgaaca
     6481 gcgtttcctt ctgattagct gcggtcaggg aaaaacgctg caatattcct gaggtctgaa
     6541 gttccggatc ctgaaatggc gacagctgcc tgtcgtcgcc gaccaatatc gttcgccttg
     6601 ccctcgccat cgggacaaga acctcagtgg gggtagcaat agaggcttcg tcgatgatac
     6661 aaaggtcata ggtgatctcg tttcttcccg gtatccccat gactccaagg catgtcccag
     6721 ccaccacctg cgcctgggtc acgagcgctg ccttgaactg ggggctgcga ccaaacctta
     6781 cttcccactc cgagtgaacc gccaacatct tgcgtagctg gattacttgt gtggatctct
     6841 ttacgtagct gtccgcccaa gacctcaatt cttcagcact gcagtcggca agatgttcca
     6901 gtgtctcctt gtcagtctca tgcttcctca gttctgccag cgattccttg agatctcttg
     6961 tcagggcatc ctgttccttg accagttccg cctgcaattc ttccatgtcg gcgcgaactt
     7021 caccttcgag ggaatttttg tcgcgggaaa gtgtcttgtc aaattcggcg atctgcgatt
     7081 ggatccgttc caggcgctcg cgaaggccgg catgtcgatc cagtgccatg gccattcgag
     7141 tgctcgtgag atcaacacca ctcttctgag cccaggactc gacgaattcg cggcccttcg
     7201 caagagccgc ttttcgcatg atcggaagct tccgatcgat caacagagtc tttgcctttt
     7261 cagtgatgcg ctcgtcactc tcattgccaa tccgaatcgc ccggacctcc tcattgctct
     7321 ccttgagtat ccgctccaga gaattatcca atgccacatg cgtctgagag gtcagcaaga
     7381 tccgggcgtt cgggttgctt cgcaatgttt ggagcacaac ctcggtaatg aaggtggttt
     7441 tgccggcacc gggagggcct ttgactagca tcagggctgg ctccgacagc gctgttcgaa
     7501 ccgcctctcg cttgtcgtca tccatcgcct gctgtatgaa actgacctcc cgggttaccg
     7561 gtaccgggac cgaggatgga tcgacgatgt agttccccag tagcggattg atcgaacgac
     7621 caaatctgac ggcttcgaca gccgtcttct ggcggtcaag ggctacgtcg gctttcacgg
     7681 tatcgactac gatggcccct tgcttatgca aactcgatgg ctccacacgg ttacgttcac
     7741 ccggctgcac tacgaccgac acgtcgctca cggaaataac cgtaccggta aaggtttcgc
     7801 ccgattcgct gtcggcctgt atgtcctgcc catcaagcgt tcgcgtgtct gcaccgttcg
     7861 ccaccaggaa gcgaatcacg cttccggaaa tctcaatatc ccgataaggg caacggcgtt
     7921 tccgctgccg ctcaagttcc gtctttgcac tcaacaagtc gagccaggtc ttgtagatcg
     7981 ccttttgccg ctgctgaaac ctgtcagcct tctgatcggc accgaactga agtaactcat
     8041 cttggagctt gacgatgttt cctgccgacg taatgggaag aacaccgcca aaactgaatt
     8101 catacctgga cggcgaggca gcctcgcgat tccgctccga ttccgaggcc gactgctcaa
     8161 gtgccgagat gagcagcagc ttctcacctg ccggaggtgc cataagcgca acatagccat
     8221 agcgaccgcc aagcaagcga atcaaccgtc ccagaggagc gctgccgtcg ggccctggcc
     8281 ggggttcagc cggatcatga atacagaaag ctgactggag gtcggcctcg acgaaattct
     8341 gaatttcggg atcgcccttc aaggcaaggt catagtcaat gatctgctta accttgttgg
     8401 tcaggccgat cagaattctc ccaaccttct gcgtgggtgg aggaggtacc aatctgtcga
     8461 tttcagccaa caggaccgct gcattgactt ggcgttccgc aggattgtcg agcgacagcg
     8521 atcgacgcag aagacgcttc aggggttcgt cgatcttgag cttttcaagg cccgcgatca
     8581 gggcattgtg attgacgatt ctttcgttgg ttatcgcagc aacggacagt gcaacaaagc
     8641 caaaaacatc gcggctatag ctatagcttc cgtcgtccat ttccggtggg gcaaacggaa
     8701 atgacgcgaa atgggccaac gttacgccgg gctccaggaa gttgcggatt tttgaaatcc
     8761 cgaaatcgca taccttcgga acaccttccg aggtaaggag aatgttggat ggcttgatgt
     8821 cccgatgcac cgttctgtgc gagtgggcga aagcgagcgc ttcaagtatc gggcgtccga
     8881 ttttggcaaa gaacaccccc caatcactca gacgagtgcg acctaggaaa ctttccagaa
     8941 catcttcgat ccactccatc gcgatgaagt gctcgttcgt atcttcatcg aaacctgaat
     9001 cgaggatctg gacgatgttc ggatgtttca agtccgatag cgccagcgtt tcgcgacgaa
     9061 acgattcctc aaccacgggg tcggcgccat ttacagggcg gaacaccttg agggcaacgt
     9121 gcttctcctc ctgggtatcg aaaaccctga agactgaagc tatgccaccg gaacgccctt
     9181 cgcccgaaat taaatagcgc ccatcggcaa gaaatctctt cttgatgtcg gatttctttt
     9241 tgacgccgtg caatgtcaaa gttccgttga ttgggttcaa tcaggatctc cctagatttg
     9301 ctatttgaat gttctctgca tgccaaagat attctcacag gtttcatctt ggtcggaaag
     9361 cgccttgaag gccgactgta tgtcaacttt ccggtgcatt gcagcctaca acgcttgcga
     9421 aactggggtc agcactggcc gaagtgccga agtagttcgt ccggcaggtt tcggagtgga
     9481 actgacgccc gaatgaccaa ggttgctagc gactgaccgg ccgcttctgg ccgtgagcgg
     9541 accagagatt tccacttggc atccaatacc gccgcggggc t
//
LOCUS       DAOQDS010000103         9132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_4031_length_9132_cov_19.788697, whole genome shotgun sequence.
ACCESSION   DAOQDS010000103 DAOQDS010000000
VERSION     DAOQDS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4031_length_9132_cov_19.788697"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="PK589_17230"
     CDS             <1..1179
                     /locus_tag="PK589_17230"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNJ21409.1"
                     /translation="KAEPSSNVVNSLRALRMAKGLSQGQLADGALITRQAVCAIEADQ
                     YLPTTAVALRLASVLGCHVEDIFSLKTTGELIEGDWVWPGLAVSAVQPRTRVKVAKVE
                     ERFVVRPVAALGEILNYTVPADGLIIGKAGSGPRSAKSDYRVLVRLLRERQIVEREIA
                     VAGCDPAINLASEYLHRHKDTSTVVGWSMGSAAALEALKRKEVHMAGLHILDAKTGES
                     NLPYLRRHLQGDDYIVVTFAAWEEGLLVRAGNPKRIRSVEDLIRPNVVVVNREEGAAA
                     RMLLDQRLEALGADGTSVKGYGVTVSSHFEVARHIAEGHGDVGIGVRSAAQIFGLDFV
                     PLQQARYDLVLPKRYLSSHPGLSHLLDAIASRQFRTEVEALGGYDMTETGKVRSLRVG
                     "
     gene            1235..2074
                     /locus_tag="PK589_17235"
     CDS             1235..2074
                     /locus_tag="PK589_17235"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNJ21410.1"
                     /translation="MKGQWRMSHRVRAFLSTWCLAVVFWCGAGQAHAAESFVIAASPS
                     LKGLLERLGSGFEQAHPEVRVKLYFDSGLGLRQTIAGMENSMVGQYFVGSGPINLVAP
                     GGDEVITRLQVKYYVLPGTKRSYAVDQLVLVVPESLVEAPGSLEAIAQGNARLAIAER
                     TRTRLGAQTADMLRASGIEEAFKGRLDMATDGRGVIDHVLSGQADVGIIFGDQAVDQQ
                     QRLRVVGVINKGYQPTVHSMAMERYCPNRRLCEEFLTYIQSAEAQRLVRQAGYAVPAM
                     SGP"
     gene            2149..2823
                     /locus_tag="PK589_17240"
     CDS             2149..2823
                     /locus_tag="PK589_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21411.1"
                     /translation="MMNSRLIRSSRRGTSVLALMLIWALAAAGCARLPYTTKTIHEDQ
                     RVVVAVQRDVQPQTYSHPVQLTSADLTSLLRGFSIREQQRVPLRWFAEEQPPKPLFRE
                     DDLHALAPYLAEGFQHVGPNDRVHFEVRNPGFNPAYGRDTIAGWMAVRDPYLYLTIEY
                     FHTQIPVRKADDYDTNYPLTPPTPKEYIIYFEPGRFYASDEKRVPAVDFRQFLKSGEA
                     GATAPR"
     gene            2887..4614
                     /locus_tag="PK589_17245"
     CDS             2887..4614
                     /locus_tag="PK589_17245"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNJ21412.1"
                     /translation="MRLREGLYIFRDQWSGHRVLLGIVVLLAACFFSAGVTEAADTGK
                     LVEKDGKYVFVESMDPSLKLLLERSVKSGMITQEEYEQVVKDSEERTQLLSPTFRGWY
                     DRGFNLSMNDNAFFLKIRFRSQLRYTQRFRNDAWRNPGEAKNFPELLGVFGDYRANRS
                     ENSASSFNVRRLRFYFMGHLFDPDFKYYIQMRAETAENAQSPGSLSLFDVNFTYTKLP
                     WANVQFGQYKVYFNRSQINSTASMQFAERALVQDAFTASGLDRRDIGITIMNDEELFP
                     VNYYFGVFNGAGPSFNRLGSFTSEEATVGCPGGQTSGNPFPSPAGCPANQRNLNANTR
                     IDVDRLMYVARLNWNILGRPGYGEGDLAYSETPQFAVGGGYSYNPAVNTSSDNAFVGL
                     DLANLNIRRQLAAFGNGRQLGLGIVDYSTWAADGVFKYRGFSLQGEFYFKNVIRHDKG
                     LPCMNAACTTTAPNNFGNAMGWYVQGGYYLIPRTVEVAARYAYWDPDTNSANDLIRQA
                     DLSLNWFLNGTYDHQIMVTYSNTQFGTGGYAIGRSAPLPPGSGTVPLDAVGGTLIENA
                     IRVQYQIFF"
     gene            4828..5586
                     /gene="modA"
                     /locus_tag="PK589_17250"
     CDS             4828..5586
                     /gene="modA"
                     /locus_tag="PK589_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNJ21413.1"
                     /translation="MRRFFAIILLILCAVSPVRAEELAVAAASDLNFAIKEVIGEFEK
                     QTGHRVKVSLGSSGNFYAQLQQGAPFDLYFSADIGYPKKLEEGGLTVPGSLYRYAVGR
                     VVLWAPKSSPLDVNKGLTVLREPTIRKIAIANPKHAPYGRAAVAAMEHAQVYADVKDR
                     LVLGENISQAAQFIESGACDVGIIALSLALAPAMKSAGSYWEIPAEAHPPLEQGAVIL
                     KQSKNQELARSFLSFLQGPQGQEIMTRYGFTLPK"
     gene            5564..5941
                     /locus_tag="PK589_17255"
     CDS             5564..5941
                     /locus_tag="PK589_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21414.1"
                     /translation="MVSHYQSKHWWLVLCGALFVTAGCSQAVLMEQESAQGGVVTYSY
                     KQDRGGPMGSPYRKQALDLMQAKCGGGSRLLREGEVRGYTSAGMGTIEGTEDEERGRR
                     WGIRFECKDREGGDKPAESLQHR"
     gene            5955..6158
                     /locus_tag="PK589_17260"
     CDS             5955..6158
                     /locus_tag="PK589_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNJ21415.1"
                     /translation="MKLSARNQFQGNVVRINEGQAMAEVVVKVGTLEFVAAITEGSVK
                     NMGLKVNDQVTVAVKATEVMIGK"
     gene            6242..6916
                     /gene="modB"
                     /locus_tag="PK589_17265"
     CDS             6242..6916
                     /gene="modB"
                     /locus_tag="PK589_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNJ21416.1"
                     /translation="MNWIAIWVTCKLAGLTAVALLFVGLPIAYWLSFSRWRWKFLIES
                     VVALPLVLPPTVLGFYILIAIGPHSPFGRLYTELVGHPLPFTFEGLLLASILYSLPFA
                     VQPFAAAFDQVDRRLIEASWTLGVSKLQTFFRLIMPLSTAGLVTGAVLSFAHTMGEFG
                     VVLMIGGNLEGSTRTVSIDIYDEVQALNYAGAAKTALFLLVVSYAVLLVVYAVNRNVW
                     AAWPRK"
     gene            6901..7995
                     /locus_tag="PK589_17270"
     CDS             6901..7995
                     /locus_tag="PK589_17270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ21417.1"
                     /translation="MAAEITIHLVKTFPGRAPIRAQIRYPIEGSTVLILFGPSGSGKT
                     TILRSVAGLEWPEEGTIRFVSRVWLDTASGIRIPPQDRHIGYMSQDYALFPTYTVAGN
                     IAYGLNHLSSAERNKRVDEVMELFQLRGLEQAKPRELSGGQQQRVALARAVAPRPLLL
                     LLDEPLSALDAPTRLHLRDELRSLLKQLALPSIIVTHDWAEALTLGDVMAVISAGDVL
                     QVGAPIDVFSRPQNADVARVVGVETVVKGRVIEATGGMLRVSVNGTTLMALESESIGP
                     DVFACIRAEDVVLEQGRASGSSARNHLTGTVQSVTMLGASARVTLDCGFPLVATVTRS
                     TVEEFKLTQGLVITAAIKAGAVHLVSRQGD"
     gene            complement(8003..8722)
                     /gene="traT"
                     /locus_tag="PK589_17275"
     CDS             complement(8003..8722)
                     /gene="traT"
                     /locus_tag="PK589_17275"
                     /inference="COORDINATES: protein motif:HMM:NF017619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HNJ21418.1"
                     /translation="MSRALSLFVGLLMLTGCSNVLRSGLVNDGSILLPPSAARTIYVQ
                     IRNTSENQTATPTDIPSRLRAKGYQVVADPMEAAYWLQTQVIYCHKAGEGVRPEAVAK
                     AGFGAGIGSGGTPLANAGGFDMEAMGGMFAGMPGGGLNAQAMRAMAMGGGGMPDMNAM
                     MRMAMNGRGGYPGMAPPPQSDDVIYLCVADVLVTEQAKNGPPRTYKMRSVAHVLQKHL
                     NIEEATPIVRDKFSASITGPF"
     gene            complement(8764..>9132)
                     /locus_tag="PK589_17280"
     CDS             complement(8764..>9132)
                     /locus_tag="PK589_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21419.1"
                     /translation="SGLHGLTGMASMAGPQGALVGGAASLGLNAAEGIGNAMGSMFGG
                     PSRPNANENINYACIADLQITDLRATGGSLPVSQPGTPPPSGVYQTRLAANVHQKKLD
                     EQEATPLVQQRLSAAVAGHF"
BASE COUNT         1800 a         2429 c         2890 g         2013 t
ORIGIN      
        1 aaagctgaac catcgtccaa tgtcgtgaat tcactgcggg cattacgtat ggccaaaggt
       61 ctctcacaag gccagcttgc ggatggggcc ttgatcaccc gtcaggcggt ctgtgccatc
      121 gaagccgatc aatatctccc gaccacggcc gtcgcccttc gacttgccag cgtgctgggt
      181 tgccacgtcg aggacatctt tagcctcaag acgaccggtg agttgatcga aggagattgg
      241 gtttggccgg ggctggctgt atcggcggtt caaccgcgta ccagggtcaa ggttgccaag
      301 gttgaggagc ggttcgtcgt ccgtcccgtg gcggccttgg gcgagatctt gaattacacc
      361 gttcctgcgg acggtctcat catcgggaag gcgggatcag gtccacgttc ggcgaaatcc
      421 gattatcgcg tgctcgtgcg cttgttgcgt gagcggcaga tcgttgaacg ggaaatcgcg
      481 gtagcgggtt gtgatccggc catcaacctc gcgagcgaat atcttcaccg tcataaagac
      541 acctccacgg tcgtcggctg gtcgatgggc agcgcggcgg cattggaggc gctcaagcgc
      601 aaggaagtcc atatggcggg cctgcacatt cttgatgcca aaacgggcga gtccaatctg
      661 ccctatctcc ggcggcatct acagggcgac gactatattg tcgtcacctt tgcggcgtgg
      721 gaagaagggc ttctggtccg agccgggaat cccaagcgaa ttcgaagcgt ggaggatctc
      781 attcgcccga atgtggttgt ggtcaatcgc gaggagggtg ctgccgcccg aatgttgctg
      841 gatcagcggc ttgaagcgtt aggcgccgac ggcaccagtg tgaaggggta cggagtgacc
      901 gtcagctcgc actttgaggt cgcacggcat attgccgagg gccatgggga tgtcggaatc
      961 ggcgtgcggt ccgctgcgca gatcttcgga ctcgatttcg tgccgttgca gcaggcccgg
     1021 tatgacctgg tcttgccgaa gcgatacctc tccagtcatc caggcctttc ccatctcctc
     1081 gacgctatcg ccagccgcca gttcagaacc gaagtcgagg cgttgggcgg gtatgacatg
     1141 acggagacgg ggaaggtacg tagcctgcgg gtcggataac gcgcctccaa gcagcaggcg
     1201 gatccgactc tgtacgatgt cgttggaggg atggatgaaa gggcagtggc gcatgagtca
     1261 tcgagtgcga gcttttctct ctacatggtg tttggccgtg gtgttctggt gcggagccgg
     1321 acaggctcat gcggccgagt catttgtcat cgcggcttcg ccgagcctca aaggattact
     1381 ggagcgattg ggcagcggtt ttgagcaagc gcatccggag gtgcgggtga agctgtattt
     1441 cgattcggga ctcggattgc gccagacgat cgccgggatg gaaaacagca tggtcgggca
     1501 gtacttcgtc ggcagcgggc cgatcaatct cgtggcgccc ggtggtgatg aagtcatcac
     1561 gcgcttgcag gtgaaatact atgtgttgcc cggtacgaag cgctcgtatg ccgtggacca
     1621 gttggtgtta gtggtgcccg agtctctggt ggaggctcca gggtcgctcg aggcgatcgc
     1681 gcagggaaac gcgcgtctag cgattgccga gcgaacgcga acgaggttgg gggcgcagac
     1741 agccgatatg ttacgtgcgt caggcataga agaagcgttc aagggacggt tggatatggc
     1801 gaccgatggg cgtggagtga tcgaccatgt gttgagcggg caagccgacg tcggcatcat
     1861 tttcggggat caggctgttg atcagcaaca gcggttgagg gtcgtcggcg tcatcaacaa
     1921 gggctaccag ccgacggtgc attccatggc gatggaacgg tactgcccca atcgccgcct
     1981 ttgtgaggaa ttcctgacct atatccagag tgcggaggcg caacggctgg tgcgacaagc
     2041 aggttatgcg gtgccggcga tgagcgggcc gtaaaaaatt ttgttcatgg tgcaaggata
     2101 catatattta atatttgtat taatgcaatg tgatcgtgcg aaggaggcat gatgaatagc
     2161 agattaatca ggagcagcag gcgagggacg tctgttctcg cccttatgct catatgggcc
     2221 ctggcagcgg caggctgtgc caggctgccc tatacgacca aaacaattca tgaggatcag
     2281 cgtgttgtgg tggcggttca gcgcgacgtg caacctcaaa cgtattcgca tccagtgcaa
     2341 ctgacgagtg ctgatctcac cagtctgttg cgagggtttt caattcgtga acagcaacgc
     2401 gtgccgttgc gctggtttgc tgaagagcaa ccaccgaagc cgctgtttcg cgaagacgat
     2461 ctgcacgcgc tcgcgccata tctcgcagag ggatttcagc acgtcggccc caacgaccgg
     2521 gtgcatttcg aggtgcgcaa ccccggcttc aatccggcct acggacgaga cacgattgcc
     2581 gggtggatgg ctgtgcgcga cccctacctg tacctcacga tcgagtattt ccatacccag
     2641 atcccggtga ggaaagcgga tgattacgac accaactatc cgctcactcc gcccacgccg
     2701 aaggaataca tcatctattt cgaaccgggc cgcttttacg cgagcgacga gaagcgcgtg
     2761 ccggcggtcg attttcggca attcttgaaa tcgggtgaag ctggtgccac ggcgcctcgc
     2821 tgacggtgga taggccgtca cacctccgat cgttcaacga actcactgac agtgatggag
     2881 ccatgtatga gactcaggga aggtctgtat atttttcgtg atcaatggtc tggtcatcgt
     2941 gtgttgctgg ggattgtcgt gctactggcg gcttgtttct tctctgcggg ggtgactgag
     3001 gcagccgata ccggcaagct ggtcgaaaaa gacgggaagt atgtctttgt cgaaagcatg
     3061 gacccgtcgc tcaaattgct gctcgaacga tcagtcaaga gcggcatgat tacccaggag
     3121 gaatacgagc aggttgtgaa ggattccgag gagcggacgc agttgctgtc gcccaccttc
     3181 cgcggctggt acgaccgcgg atttaatctc tcgatgaatg acaatgcctt ttttctcaag
     3241 atccgattcc gctctcaact ccggtatacc caacgattcc gtaacgatgc ctggagaaat
     3301 cccggcgagg ccaaaaactt tcccgaactc ctgggcgtgt tcggtgacta tcgagccaat
     3361 cggtcagaga atagtgcctc ttcattcaac gtccggcggc tgcggtttta tttcatgggc
     3421 catctcttcg acccggattt caaatactac atccagatgc gggcggagac ggccgaaaat
     3481 gcgcagtcac cgggctcgtt gtcgctgttt gatgtcaatt ttacgtacac gaaattgcca
     3541 tgggccaacg tgcagttcgg gcagtacaag gtgtacttca accggtctca aatcaattcc
     3601 acggcgtcga tgcagtttgc tgaacgagcc ttggtgcagg atgcctttac cgcgagcggt
     3661 ctcgatcggc gcgacatcgg tatcacgatc atgaacgatg aagaattgtt ccccgtgaac
     3721 tattacttcg gggtgttcaa cggagcgggt ccaagtttca atcgactggg ctcgttcacg
     3781 tctgaagaag ccacggtggg ctgccccggc ggccagacga gtggcaatcc cttcccctct
     3841 ccggcggggt gtcctgcaaa tcagcgcaat ttgaatgcga atacgcgtat cgacgtggac
     3901 cgactcatgt atgtagcgcg attgaactgg aacattcttg gacgtcccgg atatggagaa
     3961 ggcgatctcg cctattccga aacgccgcaa ttcgccgtcg gtggcggata ctcctataat
     4021 ccggcggtca ataccagttc cgacaacgca tttgtcggcc tcgatttggc caatctcaac
     4081 atcaggcgcc aattggcggc gttcggcaac ggccggcaac tcgggcttgg cattgtggac
     4141 tattccacct gggcggcgga cggcgtgttc aaatatcgcg ggttctcact ccagggcgag
     4201 ttctacttca aaaatgtcat tcgtcacgac aagggattgc cctgtatgaa cgccgcctgt
     4261 accacgacgg ccccgaataa cttcggcaat gccatgggct ggtatgtgca aggcggatat
     4321 tatttaatcc ctcggaccgt ggaagtggcg gcccggtatg cgtactggga tcctgacacc
     4381 aattccgcca acgacctcat caggcaagcc gatctgtcgt tgaactggtt cttgaacgga
     4441 acctatgacc atcaaatcat ggtgacttac agcaatacgc agtttggtac ggggggatac
     4501 gccatcggcc gaagtgcgcc gctgcctcct ggatcgggga cggtcccgct ggatgccgtg
     4561 ggggggacct tgattgaaaa tgccatacgc gttcaatatc aaatcttctt ctagcaggat
     4621 gcggaaaaag tccgccagct gcgttctcgc gtccctcaga ggctcacgta ccgcgagagt
     4681 acgattcgcc tcttcgcttg ctgcggcctt gctggacggc ctttttgcgc atcctgtggt
     4741 gtctgttgac gggacggcgt gtggatacga gtgagagtta tgccgtgaca agcaagtgtg
     4801 tccacaactt tctagggagt actgaatatg agaagatttt tcgcgatcat cctgctcatc
     4861 ttgtgtgcgg tgagtcctgt ccgagccgag gagctggccg ttgccgccgc gtctgatctc
     4921 aactttgcga ttaaggaagt gatcggcgaa tttgaaaagc aaaccggtca tcgcgtgaag
     4981 gtgtcgctgg ggtcgtcagg caacttttac gcacaactcc agcagggagc ccccttcgac
     5041 ctctattttt cagcggatat cgggtatccg aaaaaattgg aggaaggggg gctgaccgtg
     5101 cctggttcgt tgtatcggta tgcggtggga cgcgtggtgc tgtgggcgcc gaagtcctct
     5161 cctttagacg tgaacaaggg actgacggtc ctgcgggaac ctacgatcag gaagattgcc
     5221 atcgcgaatc ccaagcacgc accttacggg cgtgcggccg tggccgcgat ggagcatgcc
     5281 caagtctatg ccgatgtgaa ggatcgattg gtgctgggcg agaatatctc tcaggcggca
     5341 cagttcattg aatcgggtgc ctgcgatgtg gggattatcg cgctttcgct ggcgctggcc
     5401 ccagccatga agagtgccgg cagctattgg gaaattcctg cggaggcgca tccgccactg
     5461 gagcaggggg cggtcattct gaagcagtcg aagaatcagg aactcgcccg aagctttttg
     5521 agttttctgc aagggcctca aggccaggag atcatgaccc ggtatggttt cacattacca
     5581 aagtaaacac tggtggttgg tgctgtgcgg cgctctgttc gtgactgccg gctgttcgca
     5641 ggcggtgctc atggagcagg agtccgcgca gggaggagtg gtgacgtaca gctataagca
     5701 ggatcgggga gggccgatgg gttcgcccta tcggaagcag gcgttggatt tgatgcaggc
     5761 caagtgcggg ggcggttcac gtttgctgag agaaggcgag gtccgcggct acaccagcgc
     5821 gggcatgggt acgatcgaag gtaccgaaga tgaggaacgc ggcagacggt gggggatccg
     5881 gtttgagtgt aaggaccgtg aagggggcga caaaccagct gaatcacttc agcacagata
     5941 gaagggagtc gaacatgaag ctgagcgcaa gaaatcaatt tcaggggaac gtggtccgta
     6001 tcaatgaagg gcaggcgatg gctgaggtag tggtgaaggt gggcacgctg gagtttgtgg
     6061 ccgccattac cgaagggtcg gtgaagaaca tgggtctcaa ggtcaacgat caagtcaccg
     6121 tcgcggtgaa ggccaccgag gtgatgatcg gaaaataatc ggcggaggca gttgatcttc
     6181 ccggccggtg ttcttacgat ggcgccggtc gggaacgtct ggtcgtggct ggggcgcgcg
     6241 cgtgaactgg atcgcaattt gggtgacgtg taagttagcc ggcctcacgg cggtggcgct
     6301 cctatttgtc ggccttccca tcgcgtattg gctgagtttc tcgcgttggc ggtggaaatt
     6361 tttgatcgag tccgtggtcg cgctgcccct cgtgctccct ccaaccgttc tcggtttcta
     6421 tattcttatc gccattggtc ctcacagccc gttcgggcgt ctgtataccg aattggtggg
     6481 ccatccgctg cctttcacgt tcgaaggatt actcctcgcc tcgatactct atagtctgcc
     6541 gtttgcggtg cagccgtttg cggcggcgtt cgatcaggtg gaccggcggt tgatcgaagc
     6601 gtcctggacc ttgggggtgt cgaaactgca gacctttttt aggctgatca tgccgctatc
     6661 gacagccggc ttggtgaccg gcgccgtgtt gagttttgcc catacgatgg gcgagttcgg
     6721 cgtggtgttg atgatcggcg gcaacctcga aggttccacc cgcacggtct ccatcgacat
     6781 ctatgatgag gttcaggcgt tgaattatgc cggcgcggcc aaaacggccc tgttccttct
     6841 cgtggtgtcc tatgccgtgt tgctggtggt gtatgccgtc aatcgaaacg tgtgggcagc
     6901 atggccgcgg aaataacgat ccatctcgtg aagacgtttc ccggtcgtgc gccgatccgt
     6961 gcgcagattc gctatccgat tgagggctcg acggtcctga ttctgtttgg gccatcggga
     7021 tctgggaaga cgaccattct gcgatccgtg gcggggcttg aatggccgga agaaggcacc
     7081 atccgttttg tgtcacgggt gtggctggac accgcgtcgg ggattcggat tccgcctcaa
     7141 gatcgtcata tcggctacat gtcgcaggat tacgccttgt ttccaaccta caccgtggcg
     7201 gggaatatcg cctatggcct gaaccatctg tcttccgcag aacgcaacaa gcgggtggat
     7261 gaagtgatgg agctgtttca actgcgtggc ctggagcagg ccaaacccag agaactatcc
     7321 ggaggacaac agcagcgcgt ggcgctggcg cgagccgtgg cgccccggcc gttgttattg
     7381 ctgctggacg aaccgctgtc cgccttggac gcgccgactc gattacattt gcgcgatgaa
     7441 cttcggtcgc tgctaaagca gctggccttg ccgtcgatca ttgtcacgca cgactgggcc
     7501 gaggccctga cattgggtga tgtcatggcg gtgatcagtg caggcgacgt cttgcaggtc
     7561 ggggcgccga tcgacgtgtt cagccgtccg cagaacgcgg acgtggcgcg cgtcgtcgga
     7621 gtcgagacgg tggtaaaggg gcgggtgatc gaggccaccg gcggcatgtt gcgcgtcagc
     7681 gtgaacggga cgaccctgat ggcgctcgag tcggaatcga tcgggcctga cgtgttcgcc
     7741 tgtatcaggg cggaggatgt ggtgctcgaa caggggcgag cttctggaag cagcgcccgt
     7801 aatcacctga ccggcacggt tcaatcggtg accatgctgg gcgcatcggc gcgggtcact
     7861 ctggattgtg gcttcccctt ggtcgccacg gtgaccaggt cgacggtgga ggagttcaag
     7921 ctgacccaag ggctcgtgat tactgctgcg atcaaggccg gagcggttca tctggtatcg
     7981 aggcaaggcg attagaggtg cgctagaagg gaccggtgat gctggcgctg aacttgtcgc
     8041 gcacaatcgg cgtggcttcc tcaatgttca ggtgtttttg cagaacatgg gcgactgacc
     8101 gcatcttgta ggtgcgcggc ggaccgtttt ttgcctgttc cgtgaccagg acgtcggcca
     8161 cgcagagata aatcacatcg tcactttgtg gcggtggcgc cattcctgga tacccgccgc
     8221 ggccgttcat cgccatgcgc atcatagcat tcatgtccgg catgccgcct ccgcccatcg
     8281 ccatggcgcg catggcttgc gcgttgagac caccaccggg cattcctgcg aacataccac
     8341 ccatcgcctc catgtcgaag ccgccggcat tcgcgagtgg cgtgcctccg ctgccaatac
     8401 ccgccccgaa gccggcttta gccacggctt ccggtcttac gccctcaccc gccttgtgac
     8461 aatagatgac ttgcgtctgg agccagtagg cagcttccat cggatcggcg acgacctggt
     8521 agccttttgc tctcagccgg gatggaatat ccgtcggggt cgccgtttgg ttttcagacg
     8581 tgttgcggat ctgcacgtaa atcgttcgtg cggcgctagg gggcaggaga atcgagccat
     8641 cattgacgag tcccgaacgc aagacattac tgcaaccggt cagcatgagc agaccgacga
     8701 ataggctgag agccctgctc atggagcgga tcgccctcat gtcagctgtg ctgctcggca
     8761 tgatcaaaag tgccctgcca ctgccgcgct gaggcgttgc tgcactaagg gagtggcttc
     8821 ctgctcatca agtttctttt gatgcacgtt ggccgccagc ctggtttggt agacaccgga
     8881 cggaggcggg gtacccggtt gggatacggg aaggcttccg ccggtggcac gcaaatcggt
     8941 gatttgcaga tcggcgatgc aggcatagtt aatgttttca ttggcattgg gacgagacgg
     9001 tccgccaaac atactcccca tggcgttgcc aatcccctca gccgcattca gccccaggct
     9061 ggcggcacct ccgaccagcg caccctgcgg cccggccata ctggccatgc ccgtcaaacc
     9121 atggagcccg ga
//
LOCUS       DAOQDS010000104         8936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_4145_length_8936_cov_24.507150, whole genome shotgun sequence.
ACCESSION   DAOQDS010000104 DAOQDS010000000
VERSION     DAOQDS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8936
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4145_length_8936_cov_24.507150"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PK589_17285"
     CDS             complement(<1..276)
                     /locus_tag="PK589_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21420.1"
                     /translation="MKETVPIVTLSPRDYDAVLFDLDGVLTRTASVHAAAWKRLFDGF
                     LQQRAATTGEAFAAFDIEADYRCYVDGKPRYDGVASFLDSRGIALPSG"
     gene            complement(347..2818)
                     /locus_tag="PK589_17290"
     CDS             complement(347..2818)
                     /locus_tag="PK589_17290"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNJ21421.1"
                     /translation="MSDGLNELRQQYGCGPVQFYGTDDALYERHLVFDNVIVPEKASP
                     REQFEAVARTLRDVISQRWLLTEETYLEKNPKRVYYLSMEFLLGRSLLNNAVNSRLAA
                     LMLEAMKKKSHVDPALIEMEPDSGLGNGGLGRLAACFLDSMATLEMPGMGYGLRYEYG
                     IFKQTIKDGWQIEQADNWLRRPDPWEVARLDEAVEVKLNCSFEFRAGVLQPVIGRPSI
                     LLGIPFDRPIIGYGGKTINTLRLWAAATPDYFNFQQFSRGDFVGALAETLTAETLTRV
                     LYPDDSTYKGQGLRFLQEYFLVACSLADLVRRCRHANNDWSALPEKVAIQLNDTHPAM
                     AVPELMRILLDEAHLGWEQAWDLTQRTLAYTNHTLLPEALEKWPLRWFEVLLPRVAEI
                     ICEINRRFLDSLGTQFPNDRSRFDRVSLIEDGTDRKVRMANLAIVGSHSTNGVSAIHS
                     TLLRTRVVPDLAEIFPTRFNNKTNGVTPRRWLLLCNPGLTQLITDAIGDGWITDLSQL
                     SRLKPLAEDKGFQEGFRKAKREAKMRFADWVKATTGEVVDPDTIFDSQIKRIHEYKRQ
                     LLNALHIVVLYNRLRENPHLEFAPRTFFFAGKAAPAYALAKLIIKFINNLAGTIKGEP
                     AVGRYLRVVFVPDYCVSVAERLIPASDVSEQISTAGYEASGTSNMKFMMNGALTIGTR
                     DGATIEMAEEAGEENLFLFGLSAEEVANSRGWYNPQWHYENEPETRRALDLIFSDHFS
                     RHEPGVFNPIREALVTHGDYYMHLADLKSYSETQERLVRLYADSQGWANKAILNVASS
                     GKFSSDRTIAEYARDIWRVTPCPVP"
     gene            complement(2815..>4961)
                     /gene="glgX"
                     /locus_tag="PK589_17295"
     CDS             complement(2815..>4961)
                     /gene="glgX"
                     /locus_tag="PK589_17295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNJ21422.1"
                     /translation="EMEQKRVIQQGESFPLGSTVYPAGVNFSLFSKNSTLVELLLFDH
                     VGDAKPAAVIALDLRWNRTYHYWHVFVPGIQDGQIYGYRVFGPFEPHRGLRFDSSKLL
                     LDPYGKAMAMPDGYSRVNASVPGDNCATAMKSIVADPSKYDWEGDIPLKRPFAKTVIY
                     ELHVRGFTRHPSSGLSHETRGTYRGLIDKIPYLQDLGVTAVELLPLFQFDEQDAPRGL
                     VNYWGYSPISFFVPHHGYSSRKDHLGPMDEFRDMVKALHRAGIEIILDVVFNHTAEGD
                     HAGPTLCYRGLENAAYYMLEEDRSRYADYTGTGNTLNANQPIVRRLILDSLRYWVESM
                     HVDGFRFDLASILSRDEDGRPLKNPPILWDIESDPCLAGTKLIAEAWDAAGLYQVGSF
                     IGDSWKEWNGQFRDDVRSFMKGDNGTVSQVARRLLGSPDLYGHEEREPEQSLNFVTCH
                     DGFTLNDLVSYNQKHNEANGEDNRDGTDFNLSWNCGVEGPTDDPSIEHLRNRQVKNFL
                     ALTLLSIGTPMVLMGDEVRRTQRGNNNTYCQDNDISWFDWTRLRKHADIHRFVKFLLL
                     LRLRSPLARGESSLTLNEILCRARIDWHGIVLNRPDWNDESHSLALTAGTLDGRFLVH
                     VIVNAYWEPLAFELPGAAKSEQGWRRLIDTGLDSPDDICDGSDAPLVPGDSYLTQPRS
                     VVVLFAFKTGSVGQDISSPGNRNVWSTSAEAV"
     assembly_gap    4962..5061
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5093..7468)
                     /locus_tag="PK589_17300"
     CDS             complement(5093..7468)
                     /locus_tag="PK589_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HNJ21423.1"
                     /translation="MSRASQSPLSPEMLRKIDAYWRAANYLSVGQIYLYDNPLLKQPL
                     KRTHIKPRLLGHWGTTPGLNLIYVHLNRLIKEQDLNVIYIAGPGHGGPGLVANAYLEG
                     TYSEVYPNISQDEDGMKRLFTQFSFPGGIPSHVAPETPGSIHEGGELGYSLSHAYGAA
                     FDNPDLIVAAVVGDGEAETGPLATSWHSNKFLNPVTDGVVLPILHLNGYKIANPTVLA
                     RISHDELDHLFRGYGYTPYFVEGRDPRTMHQLMAAALDAVLEDIRRIKARARRGGAHA
                     RPLWPMIVLRTPKGWTGPKQIDGKRTEDYWRSHQVPMGDMDKPSHIKTLEKWMKSYKP
                     KELFDKTGRLKPELSELAPKGTRRMSANPHANGGLLLRDLCLPDFRDYAVKVTKPGAI
                     VAESTRLMGKFLRDTMKLNMEGRNFRLFSPDENNSNRWQDVLEVTNRTWMADRYPYDD
                     HLAPDGRVMEILSEHQCQGWLEGYLLTGRHGFFSCYEAFIHIVDSMFNQHAKWLKVCN
                     HIPWRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDHVVNKKAEVVRVYLPPDANCLL
                     SVTDHCLRSRNYVNVVVAGKQPAPQWLTMDAAVKHCSAGLGIWEWASNDRGSEPDVVM
                     ACCGDVPTLETLAATDLLRRHLPQLKIRVINVVNLMKLQPPKEHPHGLSDKEFDALFT
                     TDKPIIFAFHGYPWLIHRLTYRRTNHPHLHVRGYKEEGTTSTPFDMVVMNDLDRFHLV
                     GDVIDRLPQLGSRAAYAKQAIREKLLEHKQYIATHGDDMPEVRDWKWSRNG"
     gene            complement(7505..7849)
                     /locus_tag="PK589_17305"
     CDS             complement(7505..7849)
                     /locus_tag="PK589_17305"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ21424.1"
                     /translation="MNALARALFILLILIGAVQGCGLWAAEGGASKGKRLFEEHCMVC
                     HGQGGKGDGYRLFNPPPADLTSPAVQEKLDAALLRTIHEGRRNTAMGRWKYVLSDEEA
                     REVLAYLRSLSQ"
     gene            complement(7936..8853)
                     /locus_tag="PK589_17310"
     CDS             complement(7936..8853)
                     /locus_tag="PK589_17310"
                     /inference="COORDINATES: protein motif:HMM:NF018439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HNJ21425.1"
                     /translation="MRQGRWAVGILALAVATAGPVLAEEPPAGQAVDCRYEYATEGVT
                     GAAFPTDDVFRPLMADPKQPQFFASYLAARARTDGTHANIGSVGFGENFGFYTRREGC
                     NGWQVGLLAGVFSQFDLSAPSSDLINTDYVVGVPLSWRSGGWSTRVRLYHQSSHLGDE
                     FLLGRPGFNRLNLSFEELEALVSYDYRWGRLYAGGGYLVHREPASLDRNRVQWGVELR
                     GPTIPSPILGRALAGFLVTPVLGADFKSFEELNWVINANVVGGIEWSRQGSSRRFRFL
                     VNYYHGFNPYGQFFAQKVETVGFGFYLQL"
BASE COUNT         1747 a         2661 c         2559 g         1869 t
ORIGIN      
        1 gccggaaggc agggcaatgc cgcgcgagtc aagaaacgag gccacgccgt cgtaccgggg
       61 cttgccgtcg acgtagcacc ggtagtcggc ctcgatgtca aaggccgcga acgcttcgcc
      121 tgtggtggcg gcccgctgtt ggagaaaccc gtcgaacagc cttttccagg ccgctgcgtg
      181 cacgctcgcg gtcctagtca gcacgccgtc gagatcgaac aaaacagcgt cgtaatcacg
      241 aggagacagc gtgacaatgg gaaccgtctc tttcattgga ggtcgtgcct cagaggccgc
      301 atgggatcat gcaacacccg tttgaagacg gccaccggct tttgtgttaa gggacaggac
      361 atggtgtaac tctccaaatg tcccgagcat attccgcaat cgtgcggtcg ctggaaaact
      421 tgccggagct ggcgacattc aaaatggctt tgttcgccca gccctgcgag tcagcgtaga
      481 ggcgcaccag tctctcctgc gtctcgctgt acgacttgag gtccgccagg tgcatgtaat
      541 agtcgccgtg cgttaccagc gcctcgcgaa tcggattgaa gacccccggc tcatgacggc
      601 tgaagtggtc agaaaagatc aggtccagcg cccgacgcgt ttccggttcg ttctcatagt
      661 gccactgcgg attgtaccag ccgcggctat tggcgacctc ttcggcgctg agaccaaaga
      721 gaaagagatt ctcctctccg gcctcctcag ccatttcgat cgtcgcgccg tcgcgtgtgc
      781 cgatcgtcag cgcaccgttc atcatgaact tcatgttgct cgtcccgctg gcttcatagc
      841 cggctgtcga gatctgttcg gacacatcgc tggcgggaat caggcgctcg gccactgaga
      901 cacaataatc cggcacaaac acgactctca gatacctgcc cacggcgggc tctcccttga
      961 tggtgccggc cagattgttg atgaacttga tgatgagctt ggcgagcgcg taggccggcg
     1021 ccgccttgcc ggcgaagaag aacgtgcgcg gcgcgaactc gaggtgtgga ttctcgcgca
     1081 gacgattgta gagcaccacg atatgcaagg cattgaggag ttgtcgctta tattcgtgaa
     1141 tgcgtttgat ctggctatca aagattgtgt cggggtctac cacctcaccg gttgtcgcct
     1201 tgacccaatc cgcgaaccgc attttggctt cgcgtttggc cttgcgaaag ccttcctgaa
     1261 accccttgtc ctcggcgagc ggcttcaatc tggacaattg gctgaggtcg gtaatccagc
     1321 catcgccgat cgcatcagta ataagctgtg tcaatccggg attgcagagc agcaaccaac
     1381 ggcgaggcgt gacgccgttg gttttgttgt tgaaacgtgt cggaaatatc tcggcgagat
     1441 ccggcacaac ccgcgtccgc aacagtgtgg agtgaatggc gctgacaccg ttcgtgctgt
     1501 gtgagccgac gatggcgaga ttggccatgc gaaccttccg gtctgtgccg tcctcgatga
     1561 gactgacacg gtcgaagcgt gaacggtcgt tggggaactg tgtccctagc gagtcgagaa
     1621 aacgccggtt gatctcgcaa atgatctccg cgacccgcgg gagcaagact tcaaaccatc
     1681 tgagcggcca tttctccaat gcttcgggca agagggtatg gttcgtatac gcgagcgtgc
     1741 gctgcgtcag gtcccaggct tgctcccagc caagatgcgc ctcatcgagc aggatccgca
     1801 tgagttctgg cacggccatc gccggatgtg tgtcgttcag ttggatcgcc accttttccg
     1861 gcaaggcgct ccagtcgttg tttgcatgcc ggcagcggcg gacaagatcg gcaagcgagc
     1921 aggcgacgag gaaatactcc tgcaggaagc gtagtccctg tcccttatac gtcgaatcat
     1981 cgggatacag cacgcgtgtg agcgtttcgg cagtgagcgt ttccgccaac gctccaacaa
     2041 aatcaccgcg gctgaactgc tgaaagttaa agtagtccgg cgtggcggcg gcccagagtc
     2101 gaagcgtatt gatggtcttc cctccgtagc cgatgatggg ccggtcgaac gggatgccga
     2161 gcagaatcga cggtcggccg atgacgggtt gcagtacgcc cgcgcggaat tcaaatgaac
     2221 agttcagttt gacttccacc gcttcgtcta gccgtgctac ctcccaaggg tcaggacgcc
     2281 gcaaccagtt gtcggcctgc tcgatttgcc agccgtcttt gatcgtttgc ttgaagatgc
     2341 cgtattcata acgcagaccg taccccatgc ccggcatctc cagcgtcgcc atcgagtcca
     2401 ggaagcaggc cgccaggcgg ccaagaccgc cattgccgag ccccgagtcg ggctccatct
     2461 cgatgagggc cggatcgaca tggctcttct tcttcatggc ttcaagcatg agcgccgcga
     2521 gtcgagaatt gacggcattg ttcaggagcg atctccccag gagaaattcc atggagaggt
     2581 agtacacccg cttcgggttc ttctccaggt acgtctcttc ggtcagcagc cagcgttgcg
     2641 aaatgacgtc tcggagggtc cgtgccactg cctcaaactg ctcgcgcggg ctggccttct
     2701 cgggaacaat gacattgtcg aacacaagat gccgttcgta gagtgcgtca tccgttccgt
     2761 agaactggac cggcccgcat ccatattgtt ggcgaagctc gttcagacca tcactcatac
     2821 cgcctccgcc gaagtactcc agacgtttcg gttgcctggg gaagatatgt cttgcccgac
     2881 actgcccgtt ttgaacgcga ataagaccac caccgaccgg ggttgtgtca gatagctgtc
     2941 acccggaacg agcggcgcat cggacccgtc acagatgtca tccggcgagt ccagaccggt
     3001 gtcgatcaga cgacgccagc cctgctctga cttcgccgcg cccggcagtt cgaatgccag
     3061 gggctcccag taggcattca cgatgacatg gacgaggaac cgcccgtcaa gagtgccggc
     3121 cgtcaaggcc aggctgtggg attcgtcgtt ccagtccggc cgattgagca cgattccatg
     3181 ccagtcgatc cttgcccggc aaaggatctc attcagagtc agactgctct cccctcgcgc
     3241 tagtggactc ctcaagcgca acaagaggag gaacttcacg aagcggtgga tgtcggcatg
     3301 cttcctgagt ctcgtccagt cgaaccaact gatgtcattg tcttggcaat aggtgttgtt
     3361 gttcccgcgc tgtgtgcgcc gaacttcgtc gcccatgagt accatcggcg tgccgatgga
     3421 cagaagcgtc agcgccagga agtttttcac ctgccggttg cgcaaatgct cgatggacgg
     3481 gtcgtcggtc ggaccttcca ctccgcaatt ccagctcagg ttgaagtcgg tcccgtcccg
     3541 gttgtcctct ccgttcgctt cattgtgttt ctggttgtag gacaccaggt cgttgagcgt
     3601 gaatccgtcg tgacaggtca cgaaattgag gctctgctcg ggttctcgct cctcgtgccc
     3661 atagagatca ggactgccta acaatcgtcg ggccacctgg ctcaccgtgc cgttgtctcc
     3721 cttcatgaag ctgcggacgt catccctgaa ctggccgttc cactctttcc agctatcgcc
     3781 gataaacgag ccaacctggt acaggcccgc cgcgtcccag gcctccgcga tcagcttcgt
     3841 tccggctagg cacggatccg attcgatgtc ccacaggatg gggggattct tcaacggccg
     3901 gccatcctcg tcgcgggaga ggatcgaggc cagatcgaag cgaaagccgt ctacgtgcat
     3961 cgactccacc cagtagcgca ggctgtcgag gatcaaccgg cggacaatcg gttgattcgc
     4021 gttcaaggtg ttcccggtgc cggtatagtc ggcatagcgt gaccggtcct cctccagcat
     4081 gtaatacgcc gcgttttcca ggccgcgata gcagagcgtc gggcctgcgt ggtctccttc
     4141 cgccgtatgg ttgaacacga catcgaggat gatctcgatg cccgcccgat gaagcgcctt
     4201 gaccatgtcg cgaaactcgt ccatcgggcc caagtgatct ttgcgggaac tatacccgtg
     4261 gtgcggcacg aagaatgaga tggggctgta gccccagtaa ttgacgagac ccctcggagc
     4321 gtcctgttcg tcgaactgga acaggggcag caactccact gcggtgacgc cgagatcctg
     4381 caggtacgga atcttgtcga tcaatcctcg atacgtaccg cgcgtttcgt gagagagacc
     4441 ggaactggga tggcgggtaa acccgcgcac gtgcaactca tagatcacag tcttggcaaa
     4501 cggccgtttc agcgggatat ctccctccca gtcgtatttg ctaggatcgg cgacgatgct
     4561 cttcatcgcc gttgcgcaat tgtcacccgg tacggacgcg ttgacgcggc tgtagccgtc
     4621 cggcatggcc atcgcctttc cgtatgggtc aagcaacagt ttacttgagt caaaccgcaa
     4681 tccacggtgc ggttcgaacg gtccgaatac ccgatagccg taaatctgac cgtcctgaat
     4741 accgggcacg aagacatgcc aatagtggta tgtccggttc caccgcagat cgagggcaat
     4801 caccgccgcc ggttttgcat cgcccacgtg atcgaacagc agcaattcca caagggtact
     4861 gttcttggag aacaggctga agttgacccc cgcgggatag accgtggagc cgagaggaaa
     4921 actttcaccc tgttggatca cccgtttctg ctccatttcc annnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn ngagccgaga ggaaaacttt caccctgttg gatcacccgt
     5101 ttctgctcca tttccaatca cgcacctcag gcatatcgtc gccgtgcgtt gcgatgtact
     5161 gtttgtgttc aagcagcttc tcgcggatcg cctgtttggc gtaggccgcc cgtgatccga
     5221 gctgcggcag ccgatcgatc acatccccga ccagatggaa ccggtcgagg tcgttcatca
     5281 cgaccatatc gaacggggtc gaggtcgtcc cttcttcctt gtagccgcgc acgtgcaaat
     5341 gcgggtgatt ggtccgccga taggtcagtc tgtgaatcag ccacgggtat ccatggaagg
     5401 cgaagatgat cggtttgtcg gtcgtgaaga gcgcatcgaa ttctttgtcg ctcaatccat
     5461 ggggatgctc ctttggcggc tggagtttca tgaggttcac cacattgatg acgcgaattt
     5521 tcagttgcgg cagatggcgg cggagcaggt ctgttgcggc cagtgtttcg agcgtcggca
     5581 cgtcaccgca acaggccatc accacgtcgg gctcgcttcc cctgtcgttg ctggcccatt
     5641 cccagatccc gagaccggcg gagcaatgtt tgacggcggc gtccatcgtg agccactgcg
     5701 gagccggttg cttccccgcg acgaccacgt tgacgtagtt gcggctgcgg aggcagtgat
     5761 cggtgaccga caacaggcag ttggcgtcgg gggggagata gacccggaca acctccgcct
     5821 tcttgttgac gacatggtcg atgaacccgg ggtcctggtg actgaatcca ttgtgatcct
     5881 gccgccagac atgggaggac aacagataat tgagcgaagc gatcggtcgc cgccatggaa
     5941 tgtggttgca gaccttcagc cacttggcat gttgattgaa catcgaatcc acgatgtgaa
     6001 tgaaggcttc gtagcaggag aagaacccgt gccggccggt caggaggtac ccctccagcc
     6061 atccctggca ctggtgttcg ctcaagatct ccatcacccg accatccggc gccaggtggt
     6121 cgtcgtaggg atagcgatcc gccatccagg tgcggttggt gacctccaag acgtcttgcc
     6181 agcggttcga gttgttctcg tccgggctga agaggcggaa gtttcgacct tccatattca
     6241 acttcatcgt gtcccgcagg aatttcccca tgaggcgggt ggactcggcc acgatcgcac
     6301 ccggttttgt caccttcacg gcatagtcac gaaaatccgg cagacataag tccctgagca
     6361 gcagtccgcc gttcgcgtgc ggatttgcgc tcatccgccg cgttcccttg ggagccagtt
     6421 cgctgagttc gggcttgagt cgtccggtct tgtcgaacag ttccttcggc ttgtaactct
     6481 tcatccattt ttcgagggtc ttgatgtgcg atggtttgtc catgtccccc atcgggactt
     6541 ggtgagaacg ccagtaatcc tcggtccgct tgccgtcgat ctgtttcgga ccggtccagc
     6601 ctttcggcgt ccggagcacg atcatgggcc agagcggacg tgcgtgggcg ccccctcggc
     6661 gcgcgcgggc cttgatgcgc cggatgtcct ccagaacggc gtcgagcgcg gcggccatga
     6721 gctgatgcat cgtcctggga tcacgccctt cgacgaaata gggggtgtag ccgtacccgc
     6781 gaaacaggtg atccagttcg tcgtgactga tacgggccag cacggtggga ttcgcgatct
     6841 tgtatccgtt tagatggaga atgggaagga cgaccccgtc ggtgaccgga ttcagaaact
     6901 tgttggaatg ccaactcgtc gcgagcggac cggtttccgc ttcaccgtcg cccaccacgg
     6961 cggcgacgat caaatccgga ttgtcgaacg cggccccata cgcatgcgag agggagtaac
     7021 ccagctcgcc gccctcgtga atggagccgg gggtttccgg cgcgacgtga ctcggaattc
     7081 caccggggaa cgagaactgg gtgaacagcc gcttcatgcc gtcctcatcc tgtgagatgt
     7141 tcgggtacac ctcgctatat gtcccttcaa ggtaggcgtt ggccaccagc cccggtccgc
     7201 catgtcctgg gccggcgata tagatgacgt tcaggtcctg ttccttgatg aggcggttga
     7261 gatgcacata gatgaggttc agacccgggg tcgtgcccca atgacccagc aaccgcggtt
     7321 tgatgtgcgt gcgcttcagc ggctgtttca gcaacggatt gtcgtacagg taaatctgac
     7381 cgacggagag ataattggcc gcgcgccagt acgcgtctat cttccgcagc atctcagggc
     7441 tcagtggact ttgcgacgct ctgctcatag gcttgactcc ttcccctctt tcttcccctc
     7501 tggcttattg agagagagac cgcagatacg cgagcacttc acgggcctcc tcatcggaaa
     7561 ggacgtactt ccaccttccc atcgccgtat tccgtcgccc ctcatgaatg gtcctgagca
     7621 aggcggcgtc aagtttttcc tgaaccgcgg gggaggtcag gtcggccggt ggtggattga
     7681 acagacgata gccatccccc ttgcctccct ggccatgaca caccatacag tgctcctcga
     7741 acagccgctt tcctttggag gcaccgcctt cggcagccca caggccgcat ccctggactg
     7801 ccccaatgag tatgagtagg ataaacagcg cacgagccaa cgcattcatg tcttattcca
     7861 tttcgccgat cgtccgtgat atcggcaatc gttttctctc atacatgttc gcttatttgg
     7921 acgagcggct ctcgatcaca gctgcaggta gaacccaaat cccaccgtct cgactttctg
     7981 cgcgaagaac tgcccatagg gattgaagcc gtgatagtaa ttcaccagga agcggaagcg
     8041 gcgactcgat ccctgtctcg accactcgat cccgccgacg acattggcat tgatcaccca
     8101 gttcaactcc tcgaatgact tgaagtccgc cccgagcacc ggcgtgacga gaaaccctgc
     8161 cagcgcccgt ccaaggatcg gtgatggaat ggtggggccg cgcaactcga ctccccactg
     8221 cacgcgattc cggtcgagcg aggccggttc gcgatggacc aaataccctc cgcccgcgta
     8281 caacctcccc caccggtagt cgtaggaaac cagcgcttcc agttcttcaa aactcagatt
     8341 caaccggttg aaccccggcc ggcccagcag gaactcatcg ccgaggtgac tgctttggtg
     8401 atagagacgg actctcgtcg accagccccc ggatcgccag gacaggggaa cgcccacgac
     8461 atagtcggtg ttgatgagat ccgacgaggg cgcgctcagg tcgaattggg aaaacacccc
     8521 ggcgagaaga ccgacttgcc atccgttaca accctctcgc ctcgtataga acccgaagtt
     8581 ttccccgaac cccactgacc cgatattggc atgggtccca tccgtcctcg cgcgggcagc
     8641 caggtagctg gcaaagaact gcggttgttt cggatcggcc atcaagggtc gaaagacgtc
     8701 gtctgtggga aatgccgcgc cggtcacgcc ttcggtcgca tactcataac ggcaatcgac
     8761 ggcttgcccg gccggcggtt cctccgcgag aaccggcccc gccgtggcca ccgccagcgc
     8821 cagtatcccc acagcccatc gtccttgtct catgttcgaa acacctcgca tcgcgcgact
     8881 cattggaact ggcgcccaaa gcgccacatg tcgaagccga acaacgaacc gcccag
//
LOCUS       DAOQDS010000105         8832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_4238_length_8832_cov_20.783069, whole genome shotgun sequence.
ACCESSION   DAOQDS010000105 DAOQDS010000000
VERSION     DAOQDS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4238_length_8832_cov_20.783069"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="PK589_17315"
     CDS             <1..1455
                     /locus_tag="PK589_17315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ21426.1"
                     /translation="RRQSDGFRDRSKSQLDDFTMRFEANPDDRTRITTNISWYDETTQ
                     TPGSITPTQYRNDRTLAGKPNDEFKGQRGAIDVTASREIDAHNTTKVIVYGNVFERNW
                     YIQDQAANGTLLSTSTNFLRKFNVFGVMPQHQFKFSLFGLDQQITSGVRYHAERLTDI
                     TGIGTAGSKISRTTNNADLESVAYAAYTETAIRLTEALTISPGIRWEQVNQSRETMNA
                     VPPAGGNFKGYKTTQGLIYGTGVKYQLTPDSMLFGHVHTTFRPPTFANAVDPTSGTTQ
                     DLSAERALSSDVGIRSILVPGVSAEVALFRTEFSNQIVQQGSQFVNAGKTLMEGIEST
                     VSLDWGEIVRPLQGFVTRGSITLLRPKSLFGATDGKDLPYAPRMTFYGLVGYYHPTGA
                     SIEADAMYVAQQFTDGANTQAENALGSNGAIPSYTIWNLRLNYAPPKAKWAIFAGVRN
                     LTDESFIAQRTTGSFIGIQPGEIRNFYGGVSWRF"
     gene            1502..2311
                     /locus_tag="PK589_17320"
     CDS             1502..2311
                     /locus_tag="PK589_17320"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HNJ21427.1"
                     /translation="MNRNLFNSGLWGIVLVAGLWGAVPAQAHFVWVETEATAPADQGQ
                     PVKVYFGEYAEFLREERGGRLDSIDGVTVRVQDPKRGKIDVPLTKQVNHFAGALSSCV
                     PGRNAVVAEQAKAPVQDLRKHDLGIVKPMFYARTYFVCLEESRVNEHERDHSAPLALD
                     LIPLTTGLNLAAGRVTPGPGGEIVVKAIFNGQRLPGTQVLVHAPNGWDKELKTDADGI
                     VSFTPLWPGRYVLEMIHVEKTPGEFEGTAYEALRHRSTLALQVRAERPAEQ"
     gene            2313..3119
                     /locus_tag="PK589_17325"
     CDS             2313..3119
                     /locus_tag="PK589_17325"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNJ21428.1"
                     /translation="MSRGGSERIVRLAHRWLGMGSLGFLLLSVVTGLLWADAKFLYWD
                     DHYKEKVRPAANPGIEAARLSVDRAILAAKEAVGGRALVEHVLLRSDFGRVMYELKLR
                     VEGVPTTLLLDAINGERLSPISPDLAPVIAQQYVRTPAAVTGVELERYTPRKKKRAQD
                     AVRVRFDNADATEIVLDRQTGEILEDEGRWRRVHFAVMQLHQLNFFGFEKTLLNIPGV
                     PILLMSLSGCLLWIFHRARMRRAQLAPVSRETGLFPARSTGEPKEVPPLG"
     gene            3193..5226
                     /locus_tag="PK589_17330"
     CDS             3193..5226
                     /locus_tag="PK589_17330"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ21429.1"
                     /translation="MTIRTIKGRIVLAIVMVGCIPLLISLVLAYMSGMRSLRDVIGGN
                     FQAIAEQAADRLTMLVQSEVQGVRLLASAPLRVRQPVEAANLSYKGEWADTQRLIQER
                     AKEWEKGHDNAAGLLNLELSRFLLETKVRNGDKMVGLLITDRYGALVAASSEPDHYFL
                     SQEPWWEALQAGGLDRVYVSGLIPGQEGSFRSPEETIDIAVPILDDHQHAVIGAIKAS
                     YRFDSLFAMIKEIRIGQTGHAMLFDAAGQPLVCPILPRRAHRIPGQLMAMIVSSNPGW
                     GIAEDDGHGATDTVVGYAPVTGLSLPDNSWHVFVRQQPEETYAPIRDQLRNLALIGVV
                     MSGLLWAMGRYVATRIARPIQVLKTGVEAISRGTYDGPLDVKTGDEFEDLAVAVHRMA
                     DRLQASRIELESLNAELTHRVEEKTAEITRQMRKLELSERLATLGKVASGIAHEINNP
                     LGIILNRIECMEADAAQSNVPDEVGRDLVTIRTQAERISRVTRSILTFSRGTVSMLKP
                     LDLNCVARTCVAMAGERMTGLAVRMDLETAAALSPVMGDRDRLETVLLNVINNAIDAV
                     TASGEQGVVTIRTARVQINGDEWVQISISDTGPGVPSAIADRIFDPFFTTKPAGQGTG
                     LGLFLSYGIVSDHRGRIEVRNDSVGAVFDIYLPAVGLGESVHEGAPWGLQEKF"
     gene            5202..6620
                     /locus_tag="PK589_17335"
     CDS             5202..6620
                     /locus_tag="PK589_17335"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ21430.1"
                     /translation="MGIAGKILIVDDEQDALDNCRRILSRVPYDCLTECDPSRALEII
                     QRERPGLILTDLRMPQLDGIEVLAAAKRMDPSVQVVLLTAHATVQTAVTSMRYGAFDY
                     ITKPFTSRDLVQVARRAFGEDESTGAKQVGATEVRPPVDHGRQGTRATCARVIGESPA
                     MESVFSLIDKVAPTHSNVLVYGESGTGKELVARAIHDASLRADRPFIPVDCVSLPDTL
                     LESELFGHEKGAFTGAHVSKPGLFEVAAGGTVFLDEVSGMSQTLQSRLLRVLQERQMR
                     RVGGIRFLDVDVRVIAASNQDLEAACRRGEFREDLYYRLNVIPIVLPPLRSREGDIPI
                     LAQEFLRRFVKQQGAALGFVPNLDSTATELLCGYAWPGNVRELQNVIERAAVLADGPL
                     ITAAHLPERLRQVVVDEAGPGEAGSFKHAKQAAVTTFERSFLMELLKRNDGHMGRAAR
                     EAGVDRKTVERMVKKHGLRERS"
     gene            7115..>8832
                     /locus_tag="PK589_17340"
     CDS             7115..>8832
                     /locus_tag="PK589_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNJ21431.1"
                     /translation="MRSVRSGPKKLSQRRSQAAWKLLLGVMLPSVCVGAFQVASAAAP
                     ELHAAAHNQPAWAEQLKGQTIVEDAQEGHVERTAMMERQHQRIMDKINEQLVHDAEVQ
                     RTGGQYNNVNMLHQYGAGNQDLLLVSNSGAEPVSSMGGKCPASAPVRSYDVSAINVEI
                     TLNMWLDFYPGYMYTLTENIDKVRAEETKNKEARDKEGYDPGGVMNGLQNQFIQPLVL
                     RGNQGDCVKLTLRNQLEGDEEVSLNIHGSSMVMSATGQPATTTNPDSIAGKGKAVEME
                     WYIPPTQQEGSRQFHSYSNDRELTVMGLFGSFVIEPKGSEYLEPLGTGDPKPTKSGWQ
                     AIIKNGSGPDFREFVLFYHEVGDEAFRPLNKKGDFIPQRDPLTDVYRPVARALNYRSE
                     PFGVDNMQTQHEYFGFEDESMAYSAYTFGDPATTIPRSYLGDPAKFRLVHGGSEVFHS
                     HHPHGGSIRWPRSPRAIDEMPLWHAAKNGPVKYPVIRTKSDRVDVEVIGPSETMDLET
                     ECGSGLCQQLAGDFLFHCHVAHHYIAGMWGYWRVYNTIQQGANHNDVMPDLLELPDRK
                     GRIKVGV"
BASE COUNT         1800 a         2394 c         2819 g         1819 t
ORIGIN      
        1 cggcgccagt cagacggatt tcgagatcga agcaaatcgc agttagatga tttcacgatg
       61 cgattcgaag ccaatcctga cgatcggacg cggattacga cgaatatttc atggtacgac
      121 gaaacgacgc agacccctgg aagcatcacg cccacgcaat accgaaacga tcgtacgctg
      181 gcaggcaaac cgaacgatga attcaaaggg cagcggggag cgatcgacgt gacggccagc
      241 cgggaaatcg acgctcacaa caccaccaag gtcatcgtgt acggcaatgt cttcgagcgg
      301 aactggtata tccaggacca ggctgccaac ggcacgctgc tctccaccag cacgaacttt
      361 ttgcgcaagt tcaatgtctt cggcgtcatg ccgcaacatc agttcaaatt ttcgctcttt
      421 ggattggatc agcagatcac gtccggtgtt cggtatcacg ccgaacgact gaccgacatc
      481 accggtatcg ggacagccgg ctcaaagatc agtcgcacga cgaacaatgc cgatctggaa
      541 tcggtggcct atgccgccta tacggaaacg gcgattcgtc tgaccgaggc cctcaccatt
      601 tcgccgggca tacgctggga gcaggtcaat caaagtcgtg agacgatgaa cgccgttccg
      661 ccggcgggag ggaatttcaa gggttacaag acgacgcaag gactgattta cgggaccggc
      721 gtcaagtatc aactcacccc ggacagcatg ctgtttggcc atgttcatac gaccttccgc
      781 ccgccgacct tcgccaatgc ggtcgatccg acgagcggca ccacccaaga tctctccgca
      841 gaacgcgcgt tgagttccga tgtcggaatc cggtccatcc tggtgcccgg ggtgagtgcg
      901 gaagtcgcgt tgtttcgcac cgaattcagt aatcagattg ttcagcaggg cagccagttc
      961 gtgaatgccg gaaaaacgct gatggaggga atcgagagca ctgtcagtct cgattggggt
     1021 gaaatcgtcc gcccgctcca aggcttcgtc accagaggca gcattaccct cttacggccg
     1081 aaatccctct tcggagcgac ggacgggaaa gaccttccgt acgccccccg catgacgttc
     1141 tacggcttgg tgggatatta ccacccgacc ggtgcatcga ttgaagccga tgccatgtat
     1201 gtcgcacagc aatttaccga tggcgcgaat acccaagcgg aaaatgcctt gggttcgaac
     1261 ggtgccatcc cttcctatac gatttggaat ctccggctca attatgctcc gccgaaggcg
     1321 aagtgggcca ttttcgccgg tgtgcgcaac ctgacggatg aatcattcat cgcccaacgt
     1381 accaccggca gttttatcgg catccagccc ggtgaaatcc ggaacttcta tggcggggtc
     1441 tcctggagat tttgatggga agtcgcaacc tttgagcgaa cttcagaaag gaatgacgct
     1501 gatgaatcgg aacctattca atagtggatt gtggggaatc gtattggtgg ccgggctgtg
     1561 gggcgctgtt ccggctcagg ctcatttcgt ctgggtggaa accgaagcga cggctcccgc
     1621 ggaccagggg caaccggtga aagtgtattt cggcgagtat gcagaatttc tccgggaaga
     1681 acgtggggga cggctcgatt ccattgatgg cgtcactgtg cgtgtgcagg atccgaagcg
     1741 agggaagatc gatgtcccgt tgacgaagca ggtgaaccac tttgctggag ccctgtcatc
     1801 ctgtgtaccg gggcggaacg cggtggtcgc ggagcaggcg aaagcgccgg tgcaggatct
     1861 gcgcaagcat gacctgggca tcgtcaagcc gatgttctat gcgcggacct atttcgtctg
     1921 cctggaagaa agccgggtca acgagcatga acgtgaccac tcggcgccgt tggccttgga
     1981 tctgatcccg ctcacgacgg ggctgaattt ggcggcgggc cgcgtcactc ccggtcccgg
     2041 gggcgagatc gtggtgaaag cgatcttcaa cgggcaacgc ctgccgggga cgcaggtgct
     2101 ggtgcatgcg cccaatggct gggacaagga gctcaaaacg gatgctgacg ggattgtgtc
     2161 cttcaccccg ctgtggccgg gccggtacgt gttggagatg attcatgtcg aaaaaactcc
     2221 gggcgagttc gagggaacgg cgtatgaggc cttgcgccat cgcagcacgc tggcgcttca
     2281 ggtgagggcc gagcgcccgg cggagcaata acatgagtcg cggcgggtct gaacggattg
     2341 tgaggctggc tcatcgctgg cttggcatgg gctccctggg ctttctgttg ctctcggtcg
     2401 ttaccggcct gttgtgggcg gacgcgaagt ttctctattg ggatgaccat tataaggaga
     2461 aggtccggcc agcggcgaac cctggcatag aagcggcgag gctatccgtg gatcgtgcga
     2521 ttctcgccgc caaggaggct gtgggaggac gggcattagt cgagcacgtg ttgctgcgat
     2581 cggattttgg ccgcgtgatg tatgaactca aattgcgggt cgagggggtg ccgaccacgc
     2641 tcttgctcga tgccatcaac ggtgagcgcc tgtctcccat ttcaccggat ctggcaccgg
     2701 tgattgcaca gcagtatgta cggaccccgg cagccgtgac gggcgtggaa ctcgaacggt
     2761 ataccccgcg caagaaaaag cgggcacagg atgcggtgcg ggtgcgattc gataatgccg
     2821 atgccactga gattgtgctg gatcggcaga caggcgagat cctggaagac gaaggacgct
     2881 ggcggcgcgt gcattttgcc gtgatgcaat tgcatcagct gaatttcttt ggctttgaaa
     2941 agactctctt gaacattccc ggcgtgccga ttctcttgat gagtctctcg ggatgcctgt
     3001 tatggatctt tcatcgtgcc aggatgcggc gagcgcaatt ggcccccgtc agcagggaga
     3061 cggggttgtt tcctgcccgt tcgacgggcg aaccaaagga ggtgccaccc ctgggttgat
     3121 catcggccgg gttgaccctg cggtggcgtt ttcttccgcc ggttgtttag aatgtcctca
     3181 ctgaggtggg gaatgacgat tcgtaccatc aagggtcgca tagtgctcgc catcgtgatg
     3241 gtgggctgca ttccgctcct gatcagcctc gttctggcct acatgtccgg catgcgctcc
     3301 cttcgcgacg tcatcggcgg gaattttcaa gcgatcgccg agcaggctgc cgatcgtctg
     3361 acgatgctcg tgcagagcga agtgcaggga gtgcggttgc ttgcgtccgc gccgttacgt
     3421 gtccgtcagc cggtcgaagc cgccaatctg tcctacaaag gggagtgggc cgatacgcag
     3481 cggctgattc aggaacgcgc gaaggagtgg gagaaaggac acgacaacgc tgcgggccta
     3541 ttaaatttgg aactgtctcg atttcttctc gagacgaaag tccgtaacgg cgacaagatg
     3601 gtggggttgc tgatcaccga ccgttatggg gcattggtcg cagccagttc cgaaccggat
     3661 cattacttcc tcagccagga accgtggtgg gaggccttac aagccggagg cctcgaccgt
     3721 gtgtatgtca gcgggctaat tcccggccag gaaggatcgt ttcgctcgcc tgaagagacc
     3781 atcgacatcg cggttcccat tcttgacgac catcagcacg cggtcatcgg tgcgattaag
     3841 gcctcttatc ggttcgactc tctgtttgca atgatcaaag agatccgcat cgggcagacg
     3901 gggcatgcca tgttgttcga tgccgccggc cagccgttag tgtgccctat cttgcctcgt
     3961 cgcgcacatc ggattccggg ccagttgatg gcgatgatcg tctcgtcgaa cccaggctgg
     4021 ggcatcgccg aggacgacgg ccatggcgcg accgacacgg tcgtggggta tgcgcctgtc
     4081 acgggtttga gtctgccgga caactcctgg cacgtcttcg tgcggcaaca gccggaggaa
     4141 acctacgcgc ccattcgcga ccagttacga aatctcgccc tgatcggtgt cgtgatgtcg
     4201 ggcttgctct gggcgatggg gcggtacgtc gccaccagga ttgcccggcc gattcaagtg
     4261 ctcaagacgg gcgtggaggc gatcagtcgt ggcacctacg atgggccact cgacgtcaaa
     4321 accggtgatg aattcgagga cctggccgtc gcggtgcatc gcatggcgga tcgattgcag
     4381 gcgtcgcgta tcgaactgga gtcgctgaat gccgaactca cacatcgggt ggaagagaag
     4441 acggcggaga ttacacggca gatgcgaaaa ttggaactgt cggaacggtt ggcgacgtta
     4501 ggcaaagtgg ccagcggaat cgcgcatgag atcaacaatc cactcggcat cattctcaat
     4561 cgcatcgaat gtatggaagc tgatgccgcc cagtcgaacg tcccggatga ggtcggtcgc
     4621 gatctcgtca cgatccggac ccaggcagaa cgaatttccc gcgtcacgcg aagcattctg
     4681 acgttttccc gaggtacggt gtccatgctg aaaccgctcg acttgaattg cgtggcacga
     4741 acgtgcgtgg ccatggcagg cgaacgaatg acgggtcttg ctgtgcggat ggacttggaa
     4801 acggccgcgg cgttgtcgcc ggtgatgggt gatcgtgatc gattggaaac cgtgttgctg
     4861 aacgtgatca ataatgcgat tgacgcagtg acagcatctg gcgagcaggg agtggtcacg
     4921 atccgtaccg ctcgcgtgca gatcaatgga gatgagtggg tgcagatcag catatcggac
     4981 actggacctg gggtgccctc ggcgattgct gatcggattt ttgatccgtt ttttacgacc
     5041 aagccggccg gccagggaac cggcttgggg ctatttttga gttatggcat cgtctcggat
     5101 catcgcgggc gcatcgaggt gcggaacgat agcgtgggcg ccgtctttga catctatctg
     5161 cctgcggtgg gactgggcga gagcgtgcac gaaggagcac catggggatt gcaggaaaaa
     5221 ttctgatcgt cgatgacgag caggatgcgt tggacaattg caggcggatt ctcagccgtg
     5281 tgccgtatga ctgcctgact gagtgtgatc cgtcgcgggc gctggaaatc attcagcgcg
     5341 agcggcccgg gttgatcctg acggatttgc gaatgccaca actggacggc atcgaagtgc
     5401 tggctgcagc taagcgaatg gatccctctg tccaagtggt attgttgacg gcgcatgcga
     5461 ccgtgcagac cgcggtgacg tcaatgcgct atggcgcctt cgactacatt accaagccat
     5521 ttaccagtcg cgaccttgtc caagtggctc gccgcgcatt cggtgaggat gagtcgactg
     5581 gtgcgaagca agtcggggca accgaggttc gacctcccgt cgatcacggg cgtcagggaa
     5641 cgcgggccac ttgtgcacgg gtcattggtg agagtccagc catggaatcg gtcttcagcc
     5701 tgattgacaa agtggcgccg acgcattcca acgtgctggt ctacggggaa agcgggacgg
     5761 gaaaagaact tgtcgcgcgc gcgattcacg acgccagttt gcgggccgat cgccccttca
     5821 ttccggtgga ctgcgtctcg ttgcctgata cgctccttga atcggaactg ttcggccacg
     5881 agaagggcgc cttcaccggc gcacacgtct cgaagcccgg gctgttcgaa gtggcggcgg
     5941 gcgggaccgt ctttctggac gaggtcagcg ggatgagcca gaccctccag tcgcgattgc
     6001 tgcgggttct tcaggagcgc cagatgcggc gggtcggggg catccggttc ctggatgtcg
     6061 acgtgcgggt gattgccgca tcaaaccagg atctcgaggc cgcctgccgg cgcggagagt
     6121 ttcgcgagga cctctactat cgcctgaatg tcattcccat cgtgttgccg ccgttgcgga
     6181 gccgggaggg tgatattccg attctggccc aggaattttt gcggagattc gtgaagcagc
     6241 agggggccgc cctcgggttc gtccccaacc tggactccac ggcgacggag ttgctgtgcg
     6301 ggtatgcctg gccggggaat gtgcgcgaat tgcaaaacgt cattgaacga gctgccgtgc
     6361 tggccgatgg tccgctgatt accgccgcgc atttgcccga gcggttgcgc caggtggtgg
     6421 tggacgaggc aggaccggga gaggctggct ccttcaagca tgcgaagcaa gcggcggtca
     6481 cgaccttcga gcggagtttt ctgatggaac tgctcaagcg aaacgatggc catatggggc
     6541 gagccgctcg ggaagccggg gtggatcgaa aaacggtcga gcgcatggtc aagaagcatg
     6601 ggctccgcga gcgctcctga tccgttttct gtcacggcgt accgtggtta ttcacgatgc
     6661 ctgattcggg gtttccccct cagttttcaa ttggggcatc aatgtcccat tgggacaaat
     6721 gttccggaca ctcaccgttc gcctttcagt tcgcagcatt cctgagaatt gtggtttttt
     6781 gatggggcga cgtcgcccca tttttattct ctccttcatc ttcactgtct gaaattccca
     6841 acaaagtctg cgtttcctgg acccaatgaa tctggaacgg gagttgctct gtaactcgcc
     6901 gtcttttcat gcgcattgct tttcatgcgc gttgtgaagt gcgccatcgt cttgttatcg
     6961 cggatcaaag gtgtggaaac cgaaggaccg gcctcctggt gtttccactc catgaggtgc
     7021 aggtcttcac gggcctgcac cgtgcgcgac cggagtgcga tggcaagaga gtggggatgc
     7081 ggaggttgta tggtttcaag aggaggtagg tctaatgaga tcggtacgta gcgggcctaa
     7141 gaaactcagt cagcgcaggt cccaagcggc gtggaagctt ctcttagggg tgatgcttcc
     7201 atcggtctgt gtcggggcgt tccaggtggc ctcggcggcg gcgccggagc tgcatgcggc
     7261 cgcgcataat cagcctgcat gggctgagca attgaagggg cagaccattg tcgaggatgc
     7321 gcaggaaggc catgtcgagc gcaccgcaat gatggagcgc cagcaccagc ggatcatgga
     7381 taagatcaac gagcagttgg tccatgatgc tgaagtgcag cggacgggcg gccagtacaa
     7441 caacgtcaac atgttgcacc agtacggcgc cggtaaccag gatttgttgt tagtgtcgaa
     7501 cagcggcgct gagccggtct cgtcaatggg cgggaagtgt ccggccagcg cgccggtccg
     7561 cagctatgac gtgtcggcga tcaatgtgga aatcaccttg aacatgtggc tcgatttcta
     7621 tccgggctac atgtacacgc tgaccgagaa catcgacaag gttcgtgccg aagagaccaa
     7681 gaacaaagaa gcgcgtgaca aggaagggta cgatccgggc ggcgtcatga acggattgca
     7741 gaaccagttc attcagccat tggtccttcg cggcaatcaa ggcgattgcg tgaagctgac
     7801 gctccgcaat cagctggaag gtgacgaaga agtcagcttg aacatccatg gttccagcat
     7861 ggtcatgtcg gcgacgggac agccggccac gaccaccaac cccgacagca ttgcgggcaa
     7921 gggcaaagcg gtggagatgg aatggtatat ccccccgacg cagcaggagg gcagccgcca
     7981 gttccattcg tatagcaacg atcgtgagtt gaccgtcatg ggcctcttcg gttccttcgt
     8041 gatcgagccg aagggatctg aatacctcga gccgctggga accggcgatc ccaagccgac
     8101 caagagcggc tggcaggcga tcatcaagaa cggcagcggc ccggacttcc gggagttcgt
     8161 gctgttctat catgaagtcg gcgacgaagc gttccgccca ttgaacaaga agggcgactt
     8221 catcccgcag cgcgatccgc tcaccgacgt gtatcgtccg gtggcccgcg cgttgaacta
     8281 tcgtagcgag ccgttcggcg tcgataacat gcagacgcag cacgaatact tcggcttcga
     8341 agatgagtcg atggcctaca gcgcctatac gttcggcgat ccggccacga ccattccgcg
     8401 cagctacctc ggtgacccgg ccaaattccg gttggtgcac ggtggttccg aggtgttcca
     8461 ctcgcaccat ccgcacggtg gatcgattcg ttggccgcgg agcccgcggg ccatcgacga
     8521 aatgcccctg tggcatgcgg cgaagaacgg gcccgtgaaa tatcctgtga tccggacgaa
     8581 gtccgaccgg gtcgacgtgg aagtcattgg tccgtccgag accatggatc tggaaaccga
     8641 gtgcggatcc ggtctctgcc agcagttggc cggagacttc ctgttccact gccacgtggc
     8701 gcatcactac atcgcgggca tgtggggcta ctggcgcgtg tacaacacca ttcagcaggg
     8761 cgcgaatcac aatgacgtga tgccggacct gctggagttg ccggaccgca agggccgcat
     8821 caaggtgggt gt
//
LOCUS       DAOQDS010000106         8823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_4249_length_8823_cov_20.662181, whole genome shotgun sequence.
ACCESSION   DAOQDS010000106 DAOQDS010000000
VERSION     DAOQDS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4249_length_8823_cov_20.662181"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PK589_17345"
     CDS             complement(<1..914)
                     /locus_tag="PK589_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21432.1"
                     /translation="MRPSPEAGMGQAGRCVGSMVALCGLLLAVACGDASVDAYCEANG
                     RGNQDTLDLLTHVLEQKRNQTGIYLARARCYYFLGSYAQALQDASEVIRRLPNKADAY
                     LIRAKAGKMLGQIPQALDDYSTSIRLRPSAEAHYGRGLTYVREYQGKDREALDDFTAA
                     IRFDPKHVGAYKARAEIYSRHEQFQNALQDSEIVLKLDPATPNAYCNVGFAHYAVGHD
                     AEGRKLLNICYQKDPDPQTRGYYETEVRKVLAARQPRRNSDGGYQSDVRERTPYDREM
                     ERQQNVYESLRNSGFNDRAEACRTDGSK"
     gene            complement(1039..1767)
                     /locus_tag="PK589_17350"
     CDS             complement(1039..1767)
                     /locus_tag="PK589_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNJ21433.1"
                     /translation="MAQPALALDHDNLDPNRPIGMEDAYAIPKGEIGLEGGVRFNDRR
                     EGRTQVTFQPQIIYGAFANTQIEIQGDLFTDPHSIVGANKSGDLHLGLLYNFNTETLI
                     LPAMAVRVEADLPTGVNSKGVDTQVTGILTRSFGRLRVHLNGGYTVLGSPQGQERPGT
                     YRAVAAVSYPLGYPGSFRDTLIASLYTRQSDLHGERNHTGVEIGLRHQLTSRVVLDGG
                     LGTEFLGPANRAALLGMVGVSLGF"
     gene            complement(1821..2735)
                     /locus_tag="PK589_17355"
     CDS             complement(1821..2735)
                     /locus_tag="PK589_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21434.1"
                     /translation="MAIATVAFALAGCNQFEPRDKRFYYRALWNFSLREDLAELDSEF
                     NGVDFGHSNLYENLLLTGGQDVAAIEDRARKETLAFIATRPRLNPNEEAIAPTYMKLA
                     WRAQNTFDEAHALHRATYDIAVSDEPDKDRAIRNVLAYYKESAYAITSKQLDHRRLDS
                     FPYSKAFRQRFPLFNATIWSYHYLQVAVYDPLLAARDLTAKTQAVRPILTTYRRYLEQ
                     PPVEWTFMPLTAELSPEFAARYPDVAHIFDNLHMLHDNISDILTSERLPTWEAKRAEI
                     YRIVNTYYLATADATNPMIVQTAGEHHH"
     gene            complement(2800..3381)
                     /locus_tag="PK589_17360"
     CDS             complement(2800..3381)
                     /locus_tag="PK589_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HNJ21435.1"
                     /translation="MRHHHSAAAAVPRQAESDSQVHHHDHERPQPHGHAPHQAHSAER
                     HPTPSLDRTALMATLHCLSGCTIGELLGMAIGTALGWGNWSTIALAIALAFFFGYAMT
                     VYPLRRAGMAWGTALGLALASDTLSMTTMELADNAIMMVIPGAMDAGIFDLLFWGSLG
                     FSLIVAGIAAFPVNRWLIARGKGHALVHGHHCH"
     gene            3666..4172
                     /locus_tag="PK589_17365"
     CDS             3666..4172
                     /locus_tag="PK589_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21436.1"
                     /translation="MPSGIHRTLYTLFFLLLAPLTTQAADLPNTSLTPLRFLVGEWKG
                     TDADGKAHKSAFTLSSGGTTLTEVLTPPDSPPMTTMYYSDGDQLMLTHYCSLDNQPRM
                     RAAAVKDGDKTITFSFVDATNLKNPTDVHMHQLAIDVKDHDHFTQTWTLSKAGKDVPK
                     VFTFERVK"
     gene            4206..5063
                     /locus_tag="PK589_17370"
     CDS             4206..5063
                     /locus_tag="PK589_17370"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ21437.1"
                     /translation="MPSQTPAAVIEAQREDWNRVAAGWDKWDQFFNRTMAFINHRLVA
                     DARIRPGLRVLDLGSGTGYPAILAGEVVGADGTVVGMDLAESMLAVATRKAKTLGLHQ
                     VSFRTGDVTSLPFDSGSIDSIISRFCLMFLPEIPKATAEIARVLKPGGYVAAAVWSSP
                     DNNPFIRIPMDVIKTILPLPPPDPEAPGIFRLARPGDLAGMFERAGLTVLDDEEFTAE
                     VTYTTAEEFFRGLMDIAAPIQNLFAKLTPAQQTEAERGIIKTVNEYKRAEGVSLPIAV
                     RIVSARKPK"
     gene            complement(5131..5409)
                     /locus_tag="PK589_17375"
     CDS             complement(5131..5409)
                     /locus_tag="PK589_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21438.1"
                     /translation="MRTFMTVAAVVVCFSTALVFSADASETKAKMEKAKGDMKGNVEE
                     MKGETKAMNEELKGNDTKAEVERAKGKMKGAKERAKGKMKQLKEKATD"
     gene            5609..6043
                     /locus_tag="PK589_17380"
     CDS             5609..6043
                     /locus_tag="PK589_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21439.1"
                     /translation="MRHLILSGLLGFVILTTSGCSYLFYPHAKEFTEKAKGGNTVETL
                     INLTNMMEASAKAGQQGKGKDQALDDLHNQLHAFNNTICCVDKNKQETPAYALAVTHN
                     KELWAIFKRVWKFKDDQPQRSQHLELFVTEVRELRESLQALK"
     gene            6209..6580
                     /locus_tag="PK589_17385"
     CDS             6209..6580
                     /locus_tag="PK589_17385"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNJ21440.1"
                     /translation="MRPDSFKDFVVDQLGEVPQLMCKAMFGGHGLYQRETFFGIIHKG
                     RLYMKTNGMTQSAYTARGMQPFRPNGKQLLRHYYEVPVDILEDAEQLTDWALRAITPP
                     TAQLILPFHDNPSIKDVLTHM"
     gene            6580..7152
                     /locus_tag="PK589_17390"
     CDS             6580..7152
                     /locus_tag="PK589_17390"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNJ21441.1"
                     /translation="MNQELTTPSRRLWTIGHSTRSSDEFLALLHVHDIQRLIDVRRHA
                     GSRRYPHFHSDALAKSLPMAGLSYEELPALGGRRTARADSPNSGWKNASFRGYADYMQ
                     TEEFDRALEELMAQGENDRTAIMCAEAVPWRCHRSLVADALVARGWDVTHILGGGQGK
                     PHQLTTFAMIQAGRVIYPAPSDQCSTLRMF"
     gene            7204..7575
                     /locus_tag="PK589_17395"
     CDS             7204..7575
                     /locus_tag="PK589_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HNJ21442.1"
                     /translation="MWYRTGADFVLLLHVSFVLFVIAGGLLLLKWRGLAWIHLPAVAW
                     GVLVELTGWICPLTPLENHLRSLAGESSAEGDFVGRYLPSLLYPAALTREIQLLLGIL
                     VLLVNLALYWQVFFRTKGEQT"
     gene            complement(7587..8171)
                     /locus_tag="PK589_17400"
     CDS             complement(7587..8171)
                     /locus_tag="PK589_17400"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNJ21443.1"
                     /translation="MTDPRREEDVTELAALYALEALGDDAQRQFARSLETASASTKDE
                     VASFQDVVQELAFSGPAIAPPASLKARLMARIAQEPQEQAEGTGFSFVRSRDVSWQEL
                     APGLSMKVLFHDAAGARTTMLLRLAPGGTLIGHRHPQVEEFYVLEGSCLCAGEFLQVG
                     DYHRAEAGTVHPVTSSEQGCLALVMTSSKNEPIR"
     gene            complement(8168..8773)
                     /locus_tag="PK589_17405"
     CDS             complement(8168..8773)
                     /locus_tag="PK589_17405"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ21444.1"
                     /translation="MCDTQTVANAPSIHEQEWAGLLARIAVGDQSALAELYDASSAKV
                     FGLAMKILGDHAAAEETTLDVYTQVWRRISTYDAERGTPGSWLMTLAKHRAIDRFRSS
                     YLERGRQVPLEQAAELPGCGDTPEQYSAGLERQRLVQDALATLSAEQRQAIALAYYWG
                     MSQSEIAEQLKLPLGTVKTRIRLGMIKLREVLAPHGEGLLS"
BASE COUNT         1782 a         2696 c         2485 g         1860 t
ORIGIN      
        1 cacttcgatc catccgtccg gcatgcctcc gcacgatcgt tgaaccctga attgcgcagg
       61 ctttcgtaca cattctgctg ccgttccatt tcccgatcgt agggagtcct ttcccgcaca
      121 tccgactgat acccgccgtc ggaatttcgc cttggctgcc gcgccgccag taccttgcgc
      181 acctcggttt cataatagcc gcgcgtttgg gggtcgggat ctttctggta gcagatgttc
      241 aagagcttgc ggccttcggc gtcatgtccc acggcgtaat gggcgaatcc gacattgcag
      301 taggcgttcg gcgtcgccgg gtcgagcttc aggacaattt cagaatcctg cagggcgttc
      361 tgaaactgtt cgtggcggct gtagatttcg gcgcgggctt tgtaggcgcc gacatgtttg
      421 gggtcgaaac ggatggcggc cgtgaaatcg tcgagggctt cgcggtcctt cccttgatac
      481 tcccggacgt aggtcaatcc gcggccatag tgagcttcgg cactgggacg aagccggatc
      541 gatgtcgaat agtcgtccaa ggcttgcgga atctgaccaa gcatcttgcc ggccttggcg
      601 cggatgagat aggcgtcggc cttgttcggc agacggcgaa tgacttccga ggcatcctgc
      661 agtgcctgcg cgtacgaccc gagaaaataa taacagcggg cccgagcgag ataaataccg
      721 gtttgattac gtttttgttc gagtacgtgg gtgagaaggt cgagggtgtc ttggttgccc
      781 ctgccgttgg cttcgcagta ggcatccacc gaggcatcgc cacaggccac cgccaggaga
      841 aggccgcaca gcgcgaccat actgcctaca caacgaccgg cttgtcccat gcccgcctcc
      901 ggagacgggc gcattatctg ctcgtagaac aaacaacgtc aagactgtca tcacaactac
      961 tacccaagag attatacgag gcaacgtgcg gtggatggcc acccctgatt cgcgtcccag
     1021 ctcaactgac gaatcggcct aaaatcccaa ggagacacca accattccaa gcaaggccgc
     1081 ccgattggca ggaccgagaa attccgtccc gagcccgcca tccagcacaa cccgcgaggt
     1141 gagctgatgg cgcaagccga tttccacacc ggtgtggttt cgctctccat gaagatcgga
     1201 ctgtctggtg tagaggctgg caatcaacgt gtcgcgaaaa ctccccggat atcccagcgg
     1261 ataactgacc gccgcgacgg cacggtaggt gccgggccgc tcctgtcctt gcggagagcc
     1321 gagcacagta tacccgccat tgaggtgcac ccgcagccgc ccgaacgacc gggtcagaat
     1381 cccggtcact tgcgtatcga cgcccttcga attcacaccg gtcggcagat cagcttccac
     1441 gcgtaccgcc atggccggca gaatcagcgt ttccgtgttg aagttgtaga gcaggcccag
     1501 atggagatcc ccggatttgt tggctcccac aattgaatga gggtcagtga acaggtcgcc
     1561 ctgaatttcg atctgcgtgt tggcgaacgc gccatagatg atttgcggct gaaaggtgac
     1621 ctgcgtgcgg ccttctcgac gatcgttgaa tcgcacgccg ccttccaggc cgatttcccc
     1681 tttgggaatg gcataggcat cctccatccc gatggggcga ttcgggtcga ggttatcatg
     1741 gtccaacgct agagcgggct gggccacacc tgccaggcat gcgcagagca gccaccgcac
     1801 gattcgtgtg cccgcactcc tcaatggtga tgctccccgg ccgtctggac gatcatggga
     1861 ttcgtcgcat ccgccgtcgc caggtaatag gtattcacga tgcgatagat ttccgcccgc
     1921 ttcgcctccc acgtcggtaa ccgttcgctt gtaaggatat cgctgatatt gtcatgcagc
     1981 atgtgcagat tgtcgaagat atgcgcgaca tccggatacc gtgcggcgaa ctcggggctc
     2041 agttccgcgg tcaacggcat gaacgtccac tccacaggcg gctgctcgag ataccggcga
     2101 taggtcgtga gaatggggcg caccgcttgg gtttttgccg tcaggtcgcg agccgccagc
     2161 aacggatcgt ataccgcgac ctgcagatag tggtacgacc agatggtcgc gttgaagaga
     2221 gggaaccgct gccggaacgc tttggaatag ggaaacgaat ccaggcggcg gtggtccaat
     2281 tgcttggacg tgatggcgta cgcgctctct ttataatagg ccaatacatt ccggatcgca
     2341 cgatccttgt ccggctcatc cgacaccgca atgtcatagg tggcgcggtg aagggcatgc
     2401 gcttcgtcga acgtgttctg cgcgcgccag gccagcttca tataggtggg ggcgatggct
     2461 tcttcgttgg gattcaaccg cggtctggtc gcgataaacg caagggtttc cttgcgcgcc
     2521 cgatcctcga tagccgcgac atcctgaccg ccggtcagca gcaggttttc atacaaattg
     2581 gaatggccga agtcgacccc attaaactcg ctgtccagtt cggccagatc ttcccggagc
     2641 gaaaaattcc agagggcacg gtaatagaac cgcttgtcgc gcggctcaaa ctgattgcac
     2701 ccggccagcg cgaaggcgac cgtggctatc gccacgatcg ccatcctccc aaggatagta
     2761 ccgccagatt tcatgtcggt cgttccaact ccatacgtct tagtggcagt ggtgcccatg
     2821 caccagcgca tggcctttgc cccgcgcgat caaccatcga ttgacgggaa aggccgcgat
     2881 tccagccacg atcagcgaga agcccagact gccccagaac agcaggtcaa atatcccggc
     2941 gtccatcgcg ccgggaatga ccatcatgat ggcattgtcg gcaagttcca tggtcgtcat
     3001 cgacaacgta tctgatgcca gggccaaccc cagcgccgtt ccccatgcca taccagcccg
     3061 ccgcaaggga tagacggtca tggcatagcc gaagaagaac gccaacgcaa tagcgagggc
     3121 gatcgtagac caattgcccc agcccagcgc cgtgccgatt gccattccga gcagttcgcc
     3181 gatcgtgcac ccgctcaagc agtgcaaggt cgccatgagc gccgtgcggt cgagcgaggg
     3241 tgtcggatgc ctctctgcac tgtgcgcctg atgcggtgca tggccatgag gctgtgggcg
     3301 ttcatggtca tgatgatgaa cctgactgtc tgactctgcc tgccgtggta cggctgccgc
     3361 tgcggaatga tgatgcctca tgcgcatcct ccctgtaagt ggtgactgat accgtgcacc
     3421 actacagacg gaaaaaggcg gaaatcatta cagggacgaa aggacaccag ttcgcagggg
     3481 gtgcggactg aatcacccat ctcgtcgctc tcactgattg ttcgaatacg tgtatttctg
     3541 actcattgaa tcccctcctg ctgtttgctt ttgcgacaac gcgggaaaaa gtcttgatct
     3601 caaccttgga gacatgcttg actgaacgct gttccgcatt cttctcagtt tactggaggg
     3661 tttgcatgcc gagtggaatc catcgcactc tctatacgct cttctttctt ctcctcgctc
     3721 ccctcaccac tcaagccgcc gacctaccta ataccagcct cacgcctctc agatttctgg
     3781 tcggagaatg gaaagggacg gatgcggacg ggaaagctca caagagcgcc ttcaccctga
     3841 gctccggcgg cacgaccctc accgaagtcc tcacgcctcc tgatagcccc ccgatgacga
     3901 ccatgtacta cagcgacggc gaccaactga tgctgacgca ttattgctcg ctcgataacc
     3961 aaccgcgcat gcgcgccgcc gcggtcaaag acggagataa gaccatcacg ttttctttcg
     4021 tggatgcgac gaatctcaag aatccgaccg atgtgcacat gcatcagctg gccatcgacg
     4081 tcaaagacca cgaccatttc acccaaacct ggaccttgag caaggctgga aaggacgtgc
     4141 cgaaggtgtt tacctttgag cgcgtcaaat agctttcacg tcgtggatca acaaggagcc
     4201 tcccgatgcc atcacaaacg ccagcagccg tgatcgaagc ccagcgtgag gattggaacc
     4261 gcgtcgccgc cggctgggat aaatgggatc agttcttcaa tcgcacgatg gccttcatca
     4321 atcaccggct ggtggcggat gcgcgtattc gtccggggct tcgggtattg gacttggggt
     4381 ccgggaccgg gtatccggcg atcttggccg gtgaagtggt gggagctgac ggaacggtgg
     4441 ttggaatgga tctcgccgaa tcgatgctgg ccgtggcgac acgcaaggca aagacgctcg
     4501 gcctgcacca ggtcagcttc cgaaccggag acgtgacgtc attgccgttc gactccggct
     4561 cgatcgattc gatcatcagt cgattctgtt tgatgttcct cccggaaatt cccaaagcca
     4621 cagcggaaat cgcccgcgtg ctgaagccgg gaggatacgt cgcggccgcc gtctggtcga
     4681 gccccgacaa caatccgttc attcgtattc caatggacgt gatcaaaacc atcctgccgc
     4741 tgccgccgcc ggaccctgag gcgccgggga tattccgcct tgcgcgtccg ggcgacctgg
     4801 ccggcatgtt cgagcgcgcc ggtctcacgg tgctggacga cgaggagttt acggccgaag
     4861 tgacctatac gactgcagaa gaattttttc gcgggctgat ggatattgcc gcaccgatcc
     4921 aaaacctctt tgccaaactg accccggccc aacaaaccga ggcggagcgc gggatcatca
     4981 aaacggtaaa cgagtataaa cgagcagaag gcgtgtcgtt gccgattgcc gtgcggattg
     5041 tgagtgcgcg caagccgaag tagaacgata agtgagggcg cctgcccgcg acttgtgtgt
     5101 gtcacgggcg ggcgccgcac tcttctaaat ctagtccgtg gctttttctt ttaattgctt
     5161 catctttccc ttcgcccgct ccttggcccc cttcatcttg cccttggccc gctccacttc
     5221 ggccttcgta tcattccctt tcagttcttc attcatggcc ttggtttcgc ccttcatctc
     5281 ttccacatta cccttcatgt cacctttggc cttctccatc ttggctttgg tctcactcgc
     5341 atccgccgag aaaaccagtg ccgtcgagaa acacactact accgccgcga ctgtcatgaa
     5401 tgtacgcata tccgtcctcc gagtataggt gaaccgctgt cactaatctg cgggatccgt
     5461 ctgcatcccc ccactagaga atcccctagt atcgagatga atccgtcccc cctactgccc
     5521 agggttcccc ccagttgaac cactgcgcag gccgcgacac actcgtctcg acagcctgtg
     5581 tcttaatgtc tcagaaagga gccagctcat gcgacacctc attctttccg gcctcctggg
     5641 cttcgtgatc ctcacgacca gcggatgcag ttatctgttc tatcctcacg ccaaggagtt
     5701 tacggagaag gcgaagggtg gaaacacggt tgagacgctg atcaacctga cgaatatgat
     5761 ggaggcatct gcgaaggccg gacagcaagg caaaggcaaa gaccaggcgc ttgatgatct
     5821 gcacaatcag ctgcacgcgt ttaacaacac gatttgctgt gtcgacaaga ataagcagga
     5881 gacgccggcc tatgccctgg cggtcacgca caataaggaa ctctgggcga ttttcaaacg
     5941 ggtctggaag ttcaaggatg atcaacccca acgcagccag catttggagc tgtttgtcac
     6001 agaagtgcgg gaactccggg agtcactcca agcattgaaa tgaaggccgg aatgttgcac
     6061 agtgcgccac ggcggggagc cagccttcct gactctccgc cggtgcaacg ttcacgatgc
     6121 tcgcgcggtt cgtgagactg cccctgtcga atcgactggt gaaggcagtc catgtccacg
     6181 cggcgagcgt cgaaagggtc tctggcgcat gcgtcccgac agtttcaaag acttcgtggt
     6241 ggaccaactc ggtgaggtcc cgcaattgat gtgcaaagcc atgttcggcg ggcacggtct
     6301 ctatcagcgc gagacgttct ttggcatcat tcacaaaggc cggctctaca tgaagacaaa
     6361 cgggatgaca caatcggcct ataccgcgcg aggcatgcag ccgtttcgcc cgaacggcaa
     6421 gcaactcctc agacattatt acgaagtacc ggtggatatt ctggaagatg ccgagcagtt
     6481 gacggattgg gcgcttcggg ccattacccc accgacggcg caattgattc ttccctttca
     6541 cgacaatccc tcgatcaagg acgttctcac gcacatgtga tgaaccagga actcaccacg
     6601 ccttcccgac gcctctggac tatcgggcac tcgacccggt caagcgacga gtttctcgcc
     6661 ctccttcacg tccacgacat ccaacgactc attgatgtcc gtcgtcatgc cggctctcgg
     6721 cgatatccgc actttcattc agatgcgttg gccaagagtc tccccatggc gggactatcg
     6781 tacgaagaac tgccggccct cggcggccgt cgaacggccc gagccgactc gcctaacagc
     6841 gggtggaaga atgccagttt ccgaggatat gccgattaca tgcagacgga agagttcgac
     6901 agggcactcg aggagctgat ggcccaaggc gagaatgacc ggacagccat catgtgcgca
     6961 gaagccgtgc cctggcgttg ccatcgttca ttggtggccg atgcccttgt cgcacgtggt
     7021 tgggacgtca cccatattct gggcggaggc caggggaagc cgcaccagct cacaaccttc
     7081 gcaatgatcc aggccggccg tgtcatctat cccgccccct ctgatcagtg ctccactctc
     7141 cggatgtttt agacatttcg ctgctcattg tgttcattcc gacgttactg cacactctac
     7201 gcgatgtggt atcgaaccgg cgccgacttc gtcctgctcc tgcatgtaag ctttgtctta
     7261 ttcgtgattg ctggcgggct cctgttgttg aaatggcgag gactggcctg gatccacctg
     7321 ccggcggtgg cctggggcgt cctggtggaa ctgaccggtt ggatctgtcc tttgacgccg
     7381 cttgagaatc acttgcgaag tctggcggga gaatcgtcgg cggagggtga cttcgtcggc
     7441 cgctatcttc catccctgtt gtacccggcg gcactgacgc gcgagattca gcttctgctg
     7501 ggcatcctcg tgctgctggt gaacctggcc ctgtattggc aggtgttctt ccgaactaag
     7561 ggagagcaga catagcgatc gcttcgctat cggatcggtt cgttctttga ggaggtcatc
     7621 accagtgcca agcatccctg ctcgctggac gtgacgggat ggacggtgcc cgcctccgcc
     7681 cgatggtaat ccccgacttg caggaactct ccggcgcaga gacagctccc ctccagcaca
     7741 tagaactctt cgacttgcgg gtggcggtgg ccgatcagcg tgccaccggg cgccagcctg
     7801 agtaacatgg tcgtgcgtgc accggcggca tcgtgaaaca acaccttcat cgagagaccc
     7861 ggtgccaatt cctgccacga cacgtcccgg cttctgacga aactgaaccc ggtgccttca
     7921 gcctgctcct gcggttcctg tgcaattcgc gccatgagcc gcgccttgag ggaagccggc
     7981 ggcgcaatgg caggaccgct gaacgccagc tcctgcacca cgtcttgaaa tgacgcaacc
     8041 tcgtccttcg tggacgccga ggctgtctcc agcgaacgtg cgaactgccg ctgtgcgtcg
     8101 tcgccaagcg cctccaaggc atagagcgcc gcgagttccg tcacgtcttc ttcgcgtcga
     8161 ggatcggtca tgacaacaac ccttccccgt ggggcgcgag cacttcgcgc aacttgatca
     8221 tgcccagcct tatccgcgtc ttcaccgtcc ccagcgggag cttcaactgc tcggcaattt
     8281 cactctgact catgccccag taataggcca gggcaatcgc ctgacgctgc tccgcggaga
     8341 gcgtcgcgag ggcgtcctgc accaaccgtt gccgttccag ccccgcgctg tattgctccg
     8401 gcgtgtcgcc gcatcccggt aattcggcgg cttgctcgag cggcacctgc cgacctcgct
     8461 ctaaataact gctgcgaaac cggtcgatgg cgcgatgctt ggccagcgtc atcaaccaac
     8521 tacccggcgt gccccgctcc gcgtcatacg tcgagatgcg tcgccagacc tgcgtgtaaa
     8581 catccagggt cgtctcttcg gctgccgcat gatcgccgag aatcttcatc gccaggccga
     8641 agaccttcgc actactggca tcatacagct cggccagtgc ggattgatct cccacggcaa
     8701 tacgagcgag gagtcccgcc cactcctgtt cgtgaatcga tggtgcgttg gccacggtct
     8761 gggtgtcaca catggggtgt cctcgtgacg ttctacgagc gcacccgttc gtttggatgt
     8821 tca
//
LOCUS       DAOQDS010000107         7675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_5376_length_7675_cov_22.421522, whole genome shotgun sequence.
ACCESSION   DAOQDS010000107 DAOQDS010000000
VERSION     DAOQDS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5376_length_7675_cov_22.421522"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..4070)
                     /gene="rpoB"
                     /locus_tag="PK589_17410"
     CDS             complement(111..4070)
                     /gene="rpoB"
                     /locus_tag="PK589_17410"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNJ21445.1"
                     /translation="MSESTLSEFVERKDFSRIRTSIDIPDLIEIQKRSYEEFLQMEVE
                     PDRRKDQGLQAALASVFPITDYNNTAALEFSNYSLGTPKYDERECLEQGMTFAVPLKL
                     RVRLIVFDKEDKGPKKKVLDVREQEVYVGELPLMTERGTFLINGTERVVVSQLHRSPG
                     ASFTHDKGRTHASGKVLYSARIIPYRGSWLDFEFDARDILYVRIDRRRKMPATILLKA
                     FGYSTDDLLRMYYPVEEIRVSKGKLFRKLDPEIHHGLKCSTEVMEKGGKDPIVREGAK
                     LTKVLIAKLKAAGIKEIPVTPAELVGRAVLTDLVDGGKKQALAEKNQRLTEEILEKIL
                     DSDIEEFKVIYLDTTNATLVILDTLDMERTGSKEEAMVEIYRRLRPGETPSVETARAL
                     FDNLFLSPKRYDLSPVGRLKLNKKLGLDFALEQRTLTAQDIVEVVRYLVNLKIGRGEI
                     DDIDHLGNRRVRSVGELLENQFRLGLVRMERSIKERMNLLDMETVLPHDLINAKPVVA
                     AVKEFFSSSQLSQFMDQTNPLAEITHKRRLSALGPGGLTRERAGFEVRDVHPSHYSRI
                     CPIETPEGPNIGLITSLATYARINEFGFIEAPYRKVVKGRVSDELEYLSAIEGDKYII
                     AQANSKVDGSNRLVSETVSARSAGDFITATPDKIEYMDVSPKQVVSVATALVPFLEHD
                     DANRALMGSNMQRQAVPLLKAESPLVGTGMEAVVARDSGYVVQAKREGVVESVDATRI
                     VVRADAKEGRKRNDSGLDVYEMIKFQRSNQNTCITQTPVVRIGEPVKRGQVLADGPAI
                     DHGELALGKNVLVAFMPWGGYNFEDAILLSEKLVREDAFTSIHIEEFEVEARDTKLGK
                     EDITRDIPNVGEEALRDLDESGIIRIGAEVKPGDILVGKVTPKGETQLTPEEKLLRAI
                     FGEKAGDVKDTSLTVPPGVEGIVVDVKIFSRKGLDKDERSKSIESEDHMKLQRDHQEE
                     LRIIEDEKTKKVRKLLLGKVVGRDLMDPETGDVILKKKGKLTAEILKRLPDDMVRHII
                     LSDPDEQKELEDVERRAKEQIEILQTLYDEKVGRLRRGDELPPGVIKLVKVYIAMKRK
                     IQVGDKMAGRHGNKGVVSRVLPEEDMPYLPDGTPVEIVLNPLGVPSRMNVGQILETHL
                     GWAARALGIKVASPVFDGASEKEIKELLKKAKLSPSGQTVLMDGRTGESFSSPVTVGY
                     MYVLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALQAYGAASIL
                     QEFLTVKSDDVPGRSRMYEAIVKGEPFLEPGLPESFNVLVKELQSLGLDVELVKSKD"
     gene            complement(4151..4552)
                     /gene="rplL"
                     /locus_tag="PK589_17415"
     CDS             complement(4151..4552)
                     /gene="rplL"
                     /locus_tag="PK589_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNJ21446.1"
                     /translation="MSAAAGTKLSQEELIKAIEGMSVLELADLVKGLETRFGVTAAAP
                     VAVAAAGGGGAAAAPAEEKTSFDVILASAPADKKIQVIKVVRELTSLGLKEAKDLVEG
                     APKPIKSGATKEEADTMKKKLEESGAKVEIK"
     gene            complement(4580..5086)
                     /gene="rplJ"
                     /locus_tag="PK589_17420"
                     /pseudo
     CDS             complement(4580..5086)
                     /gene="rplJ"
                     /locus_tag="PK589_17420"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
     gene            complement(5118..5810)
                     /gene="rplA"
                     /locus_tag="PK589_17425"
     CDS             complement(5118..5810)
                     /gene="rplA"
                     /locus_tag="PK589_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNJ21447.1"
                     /translation="MGKKMTAAQEKIEPRFYELKEAVEAVKQAAFAKYDESVDLAIRL
                     GVDPKRSDQMVRGTTALPHGTGKKLRVLVFAKGEKEQEARQAGADYVGSDDLMEKIKG
                     GWMDFDCAISTPDLMASVGKLGKVLGPRGLMPNPKTGTVTFEVGKAVSEIRKGRVEFK
                     VEKAGIVQVPVGKVSFDVQKLYDNAQAVLESVVKAKPSSCKGRYIKSVTMSSTMGPGV
                     KLDPVALSKLWS"
     gene            complement(5855..6277)
                     /gene="rplK"
                     /locus_tag="PK589_17430"
     CDS             complement(5855..6277)
                     /gene="rplK"
                     /locus_tag="PK589_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNJ21448.1"
                     /translation="MAKEVSAQIKLQIPAGKANPAPPVGPSLGQHGVNIMEFCKQFNA
                     KTQKDGDSIIPVIITVYKDRSFTFIMKTPPASDLLKKAAGIIKGSGVPHKDKVGKVSQ
                     AQVREIAQKKLSDLNAADLEGAIKIIQGTARSMGITVQ"
     gene            complement(6353..6895)
                     /gene="nusG"
                     /locus_tag="PK589_17435"
     CDS             complement(6353..6895)
                     /gene="nusG"
                     /locus_tag="PK589_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNJ21449.1"
                     /translation="MMSNKNWYVIHTYAGFEGRVKTSLLERANQMGLTERLGQVLVPT
                     EDVIEIKDGKRRTSRRKFFPGYVLVELEAPLADETLQMIKETPKVTGFVGGGAQPTPL
                     SSEEVDSLLKQVDAGAAGPREQVRFIKGDNVRIVDGPFLGFNGAVDDVDADHSRVKVF
                     VSIFGRSTPVELGFLQVERI"
     gene            complement(6965..7159)
                     /gene="secE"
                     /locus_tag="PK589_17440"
     CDS             complement(6965..7159)
                     /gene="secE"
                     /locus_tag="PK589_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNJ21450.1"
                     /translation="MFQRLIASVREFIDGVRGELRKVSFPTKTETIGATTVVIVFCIL
                     MSLYLSMMDSVLGWLMRKVI"
     gene            complement(7252..7326)
                     /locus_tag="PK589_17445"
     tRNA            complement(7252..7326)
                     /locus_tag="PK589_17445"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7291..7293),aa:Trp,seq:cca)
     gene            complement(7383..7532)
                     /gene="rpmG"
                     /locus_tag="PK589_17450"
     CDS             complement(7383..7532)
                     /gene="rpmG"
                     /locus_tag="PK589_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNJ21451.1"
                     /translation="MREIIDLACTVCKQRNYTTRKNKKNDPDRLERNKFCKFCRKHIA
                     HKEVK"
BASE COUNT         1612 a         2429 c         1834 g         1800 t
ORIGIN      
        1 tcgaataatg tgtatacacc ttccaaggtt gatccctcct taacggtcgc ctgagcgcag
       61 ttctgagtgg tgagctatcc gatcgttgct ctcaagactc agcactagaa tcagtccttt
      121 gatttaacta gctctacgtc gagcccaaga ctttgcagct ctttgaccag cacgttgaac
      181 gactccggca atccaggttc caggaacggc tctcccttca caatcgcctc atacatgcgc
      241 gaccggccgg ggacatcgtc tgatttcacc gtcaggaatt cctggagaat ggaggcggcc
      301 ccataggcct gcaaggccca cacttccatc tctcccaatc gttgaccacc gaattgggcc
      361 ttaccaccca acggttgttg agtcaccaac gaatagggac cgatggagcg tgcgtggatc
      421 ttgtcatcca ccaagtggtg aagcttcaac acatacatat atcccaccgt gaccggactg
      481 ctgaacgatt cgccggttcg gccgtccatc aacacggttt gaccgcttgg cgacagcttc
      541 gctttcttga gaagctcctt aatctccttc tctgacgcgc cgtcgaacac tgggctggct
      601 actttaattc ccaatgcccg agccgcccaa ccaagatggg tctccaggat ttgacccacg
      661 ttcatacgag acggcacgcc cagcggattc aacacgattt ctaccggcgt accatccggc
      721 agatagggca tatcctcttc aggcaatacg cgtgacacga cgcctttgtt gccgtggcga
      781 cccgccattt tgtcgccgac ttggatcttc cgcttcatcg cgatgtagac cttcaccaac
      841 ttgatcacac caggcggaag ctcgtctccc cgtctcagcc gaccgacttt ttcatcgtac
      901 aaggtctgca aaatctcgat ctgctccttg gcacgtcgct cgacgtcttc caattccttc
      961 tgctcatccg gatcgctgag gatgatgtgg cgcaccatat catccggcaa acgcttcagg
     1021 atctccgcag tgagcttccc ttttttcttc aggatgacat cgcccgtctc ggggtccatc
     1081 agatctcgac cgaccacttt tccgagcagg agcttgcgaa ccttcttcgt cttttcatcc
     1141 tcaatgatgc gcagctcttc ctgatggtcg cgctgcagct tcatgtgatc ttcgctctcg
     1201 atgctcttcg agcgctcgtc cttatccagg cccttgcggg agaaaatctt cacatcgacg
     1261 acgattccct ccacaccggg aggaaccgtg agagaggtat ccttgacgtc gccggccttt
     1321 tcgccgaaga tcgcccgcag caatttctct tccggcgtta actgggtttc gcccttcggc
     1381 gtgaccttgc cgaccaaaat gtcgccaggc tttacttcgg cgccgatgcg gataatgccg
     1441 ctctcatcca aatcgcgaag cgcttcctct ccgacatttg gaatgtcgcg cgtgatatct
     1501 tccttaccca gcttggtgtc gcgcgcctct acctcgaact cttcgatatg gatggaggtg
     1561 aacgcatctt cccgcacgag tttttcactg agtagaatcg cgtcttcgaa gttatagccg
     1621 ccccacggca tgaacgcgac gaggacattc ttacccagcg ccaattcacc gtgatcgatc
     1681 gccggaccgt cggccagtac ttgccctcgc ttgaccggct caccgatgcg gaccacaggc
     1741 gtctgggtga tgcacgtatt ttgattcgac cgctggaact tgatcatttc atacacatca
     1801 agtcctgaat cgttccgctt gcgaccttcc ttggcatcgg cacgcaccac aatgcgcgtc
     1861 gcatcaacac tttccacgac accttcacgc ttagcctgca cgacgtatcc ggaatcgcgc
     1921 gcgaccacag cttccatgcc ggttccaact aacggagact cagccttgag cagcggcacg
     1981 gcctggcgct gcatgttcga tcccatcaat gcgcggttcg cgtcgtcgtg ctcaaggaac
     2041 ggcaccaatg ccgtcgcgac gctgacgacc tgcttcgggg acacgtccat gtattcaatc
     2101 ttgtcgggag tagcggtaat aaaatcaccg gcagagcgcg ccgaaacggt ttcagacacg
     2161 agccgattgg agccatccac tttcgagttg gcctgggcga tgatgtactt gtcaccctcg
     2221 atggccgaga ggtactccaa ctcgtcgctg acacgcccct tcacgacctt tcggtaaggc
     2281 gcttcgatga agccgaactc gttgatgcgc gcataggtcg ccaaagaagt gatcaatccg
     2341 atgttcggac cttccggcgt ttcaatcggg caaatacgac tgtagtggga cggatggacg
     2401 tcacgcactt cgaagccggc acgctcgcgg gtcaatccgc caggccccag tgccgacaag
     2461 cggcgcttgt gtgtaatttc agccagcgga ttggtctggt ccatgaattg agagagctga
     2521 ctgctgctga agaactcttt caccgccgcc accacgggct ttgcgttgat gaggtcatgg
     2581 ggcaacacgg tctccatgtc caacaggttc atgcgctctt taatgctgcg ctccatgcgc
     2641 acaaggccga gacggaattg attctccaac aactcgccga ccgatcgcac acgacgattc
     2701 cccaagtggt cgatgtcatc gatttcccct cggccgatct taaggttcac caggtatcgg
     2761 acgacttcga cgatatcctg cgcagtcagc gtgcgttgct ccagagcgaa atcgagtccc
     2821 agttttttat tcagcttgag gcggcctacg ggagacaaat cgtaacgctt gggactcaaa
     2881 aataaattat cgaacagcgc gcgcgccgtt tcgactgacg gagtttctcc cggcctgaga
     2941 cggcgataga tctcgaccat cgcctcctcc ttcgagccgg ttcgctccat atcgagggta
     3001 tccaaaatga ccagggtcgc attggttgta tccagataga tgaccttgaa ttcctcgatg
     3061 tcgctatcca gaattttctc caaaatctct tcggtcagcc gctgattctt ctccgccagg
     3121 gcctgctttt tcccgccgtc gaccaaatcc gtcaggaccg cccgccccac caactcggca
     3181 ggagtcacgg gaatttcctt gatccccgct gcctttaatt tggcaatcag aaccttcgtc
     3241 agcttcgctc cttcgcggac aatcggatcc ttgccgccct tctccatcac ctctgtcgag
     3301 cacttcagcc cgtgatggat ttcaggatcg agctttcgaa acaatttgcc cttggaaacg
     3361 cggatttctt cgaccggata gtacatccgg agcaagtcgt cggtgctata cccaaaggcc
     3421 ttcaggagga tggtcgccgg cattttccgt cggcgatcga tgcggacgta caagatatcg
     3481 cgcgcgtcga actcaaaatc gagccacgat ccgcgatacg gaataattct ggcagagtac
     3541 aacactttgc cgctggcgtg cgtgcgcccc ttgtcatgag taaaggacgc accgggcgag
     3601 cggtgcaact gactgaccac cactcgctcg gtcccgttaa tgagaaacgt tccgcgttca
     3661 gtcatcaggg gcaattcgcc gacataaacc tcttgctctc gaacgtccaa cacctttttc
     3721 ttgggaccct tgtcttcttt atcaaaaaca atcagtcgca cacgcagttt caagggaacg
     3781 gcaaaggtca ttccctgctc aagacattcc cgctcatcgt acttgggagt tcccaacgag
     3841 tagtttgaaa actccaacgc cgcagtgttg ttgtagtcgg tgatgggaaa gacgctggct
     3901 aacgccgcct gcaacccttg atccttgcga cggtcgggct cgacttccat ctggaggaac
     3961 tcttcgtagg atcgcttctg gatttcgatg agatcgggaa tatcgatact cgtgcgaatc
     4021 cgagaaaaat ccttacgttc gacaaactcc gaaagagtcg attccgacat tcgttagccc
     4081 tccaagctca taagcagatg aacgcctcgc gccatctcaa cggacaatca cactgcagcg
     4141 cccgaaacaa ttacttgatt tcgactttag ctccgctctc ttcgagcttc ttcttcatcg
     4201 tgtcggcttc ttccttggtg gcgccggact tgatgggctt cggtgcgcct tcgaccaggt
     4261 ccttggcttc cttgagtccg aggctggtga gctcgcgcac caccttgatg acctggatct
     4321 tcttgtcggc cggagcgctc gccaaaatca cgtcgaatga cgtcttttct tcggccggag
     4381 ccgctgccgc accgccgcca cccgctgccg ccacggccac cggggccgcc gctgtcacgc
     4441 cgaaacgagt ctcgagaccc ttcaccaggt cggccaattc caacacgctc atgccttcga
     4501 ttgccttgat caattcttcc tgtgacaact tggttcctgc tgctgctgac atgtcgccct
     4561 cccctttctt tttatcctga atggctgcaa tgactcgcac aaacttgctc aatactccac
     4621 ttaacgcata gaccacgccg cgtatcggac cctgcatggc cgatagcaac attgcaatca
     4681 acacttcctt cttcggcaat tgcgcaatgg ctgcaaggtc tgcagcctgc accaacttgc
     4741 cctctaacac gccgacggtc accttgatct tctcagatcg cttttcggcc tgaataaaat
     4801 cgcgcaagat tttcgccggc aaaaccggat cgtcgtatcc gatcaccagc ccggtcgggc
     4861 ccttgaagtg atcttttgca tcggcgagga tcgtaccggt cgaggcgcgg acggccaggg
     4921 tattcttgat gacacagtac tcggctttag ccccgcgcaa ctgccttcgc agctcggtca
     4981 tttgattgac gggcatcccg gcgcattccg tcaaaatggc caagcgggcg cggccaaact
     5041 tctctgtcaa ctccgcgatc gctgttgcct tctcttcttt cttcatacct ctccccttac
     5101 tgagaacctg tccgttctca actccacaac ttcgacaacg ccacaggatc cagcttcacg
     5161 ccaggcccca tcgtgctcga catcgtcaca ctcttgatgt accggccctt gcaggatgac
     5221 ggcttcgcct tgaccaccga ctccagcact gcctgcgcat tgtcgtacag cttctgaaca
     5281 tcgaacgaca ccttgccaac cggcacctgg acgatgccgg ctttttccac cttaaactcg
     5341 acacggcctt tgcgaatctc gctcacggcc ttccccacct caaacgtcac agtgccggtc
     5401 ttcggattgg gcatgaggcc acggggacca agcaccttac caagcttacc gacagaggcc
     5461 atgagatcag gggtggaaat cgcacaatcg aaatccatcc atccgccctt aattttttcc
     5521 atcagatcat cagagcctac gtaatcagcc ccggcctgcc gcgcttcctg ctccttttca
     5581 cccttggcga atacgagcac ccggagcttt ttgccggtgc catgcgggag cgccgtggtt
     5641 ccacgtacca tttgatccga tcgcttggga tccacaccca gccggatcgc caggtcgacg
     5701 gattcatcat acttggcaaa ggctgcttgc ttgacggcct caaccgcctc cttcaactca
     5761 taaaacctgg gctcaatttt ctcctgagcc gcggtcatct tctttcccat actgctgctc
     5821 cttcacatcc gccggaggtt cactctgcac gaatttactg gacggtgatg cccatgctgc
     5881 gcgctgtgcc ctgaataatc ttgatggcgc cttcaagatc agccgcgttc aagtccgaca
     5941 acttcttttg cgcgatttcc cgaacctgag cctgactgac cttgcccacc ttatccttgt
     6001 gcgggacgcc cgatcccttg atgatacccg cggccttctt gagcaagtcc gacgccggag
     6061 gcgtcttcat gatgaaggta aagctccggt ccttgtagac cgtaatgatc accgggatga
     6121 tgctatcccc atctttttgg gtcttcgcat tgaattgctt gcagaactcc atgatgttca
     6181 caccgtgttg gcccaacgaa ggaccgaccg gcggagcagg attggcctta ccggccggga
     6241 tctgcaattt aatttgagct gatacttcct tggccatgag ttactccctg agaactgcgg
     6301 actgcgcgag gaccggaaat agctcccagc ccactcccac caatccagtg actcaaatac
     6361 gttcgacctg caagaatcca agctcgaccg gagtagaccg gccgaaaata ctgacaaaca
     6421 ccttcacgcg actgtgatcg gcatccacat catcgacggc gccattgaag cccaggaacg
     6481 gaccatccac gatacgaacg ttgtcccctt taatgaaccg gacttgctct cgaggtccgg
     6541 ccgctccggc atcaacttgc ttcaacaaag aatcaacttc ttccgaggag agcggcgttg
     6601 gctgtgcccc tccaccaaca aaccccgtga ctttcggggt ctccttgatc atctgcaagg
     6661 tttcgtcagc caacggcgct tccagctcga cgaggacata gcccggaaaa aattttcgcc
     6721 gggaggtccg tcgcttgcca tccttgattt cgatgacatc ttccgtagga accaggacct
     6781 gcccaagtcg ctcggtgagc cccatttgat ttgcgcgctc gaggagactg gtcttcacgc
     6841 gaccctcaaa ccccgcatag gtatggatga cgtaccagtt cttattgctc atcatctcct
     6901 ctcagctgaa catcagcatc gtatcgtccg ggcaatgccg gacctgtcca gccgcaccac
     6961 gcaactaaat aaccttgcgc atcagccagc ccagcacgga atccatcatc gacagataca
     7021 aggacatgag aatgcagaac acaatcacga ccgtggtagc accgatcgtt tccgttttcg
     7081 tcggaaaaga aaccttccgc agttctccgc gcacaccatc gataaattct cgaacagagg
     7141 caatcaatcg ctgaaacacg gttcaaatcc tctcgtagaa cttttcgact gccagactcc
     7201 cccagacgga ccaaccaccc tacacatcac cccactaaga agcatccgct ttggcagggg
     7261 cactaggatt tgaacctaga ctctcggttt tggagaccga tgtgctgccg ttgacaccat
     7321 gcccctccgc tcggaacatg agcgccgcgg ggcccgcaac gaggccccgc gccccacacc
     7381 ccttacttca cttccttatg ggcaatgtgt ttgcggcaaa acttgcagaa cttgtttcgc
     7441 tccagccggt ccggatcgtt tttcttgttc ttgcgggtcg tataattccg ctgcttacag
     7501 accgtacagg ccaagtcaat gatttctcgc atgtcgtata cccgtgaagc gtgtaacgtg
     7561 aagccggaga gtgggattgt ctccgcgctc gaaacgcctg acgcttaacg cctccctcta
     7621 ctaagctaga atttccgtaa cgacgcccga gccgacagtc ttgccgcctt cgcgg
//
LOCUS       DAOQDS010000108         7627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_5449_length_7627_cov_19.001057, whole genome shotgun sequence.
ACCESSION   DAOQDS010000108 DAOQDS010000000
VERSION     DAOQDS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5449_length_7627_cov_19.001057"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PK589_17455"
     CDS             complement(<1..241)
                     /locus_tag="PK589_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HNJ21452.1"
                     /translation="MYPSNRHSIETLLKTPIGRRTALALGARAASLLATTMGLGAATG
                     LLQSLGGCQRAPGTARDQFIYLSEEKEMAMGLSAFR"
     gene            complement(292..1281)
                     /gene="sppA"
                     /locus_tag="PK589_17460"
     CDS             complement(292..1281)
                     /gene="sppA"
                     /locus_tag="PK589_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNJ21453.1"
                     /translation="MKRNVGQAPDVWQLSTGLLVLALALMQAACVTINLPPGPGALEE
                     HKVSGTGKDKVLLMDVSGVISSENKDGFYSSPGMLATVKEELERAAKDERVKAIVLRI
                     NSPGGTVTASDIIYHELRNFKTSRKIPIVASIMDVGASGGYYIAAAADTVLAHPSSVT
                     GSIGVIMLTVNARGLLEKVGVETNAVTSGPRKDMGSPFRAMLPEERAIFQGVIDGFYQ
                     RFLQVVQEGRPGMNGETIKKLADGRIYSGEQAKAAGLVDDIGYLDDAIELAKKKAGLT
                     DAKVVTYRRPGEYQNNVYSRLVAPAPSLASLANVDLLSVVRGGSPQFMYLWMP"
     gene            complement(1439..2623)
                     /gene="carA"
                     /locus_tag="PK589_17465"
     CDS             complement(1439..2623)
                     /gene="carA"
                     /locus_tag="PK589_17465"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNJ21454.1"
                     /translation="MKKAILALADGTWFEGRALGAEGETGGEVVFNTAMTGYQEVLTD
                     PSYRGQIVTMTSPQIGNYGVTPEDVESTKIWAEGFIVKESSRLASNWRGKSTLQEYLQ
                     AAHIVAIEGIDTRALTRHLRERGAQPGVISHIDLDPRRVAEKARQAPSIIGRDLAATV
                     TCEHRYPWTEGTGDWAPKITMPEQGAAKAPRKTWRVVAYDFGVKQNILRRLVDVGCEV
                     TVVPASTPAKDVLALNPQGLFLSNGPGDPEGVPYAMTALRELIGRVPIFGICLGHQLL
                     GLALGFSTYKLKFGHHGANHPVIDLRTRKVEITSQNHNFAVRFPGETPVVGQELPIVD
                     TPFGRVQLTHTSLNDASVEGMRCLDRPVFSVQYHPEASPGPHDSAYLFEQFVALMETH
                     HA"
     gene            complement(2626..3135)
                     /locus_tag="PK589_17470"
     CDS             complement(2626..3135)
                     /locus_tag="PK589_17470"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNJ21455.1"
                     /translation="MRQGLIACVTFEMLALAVWIGGLLVLVAAVIPAVFNTFGGQDTG
                     GFFLTRAFDGYNRLVLGAAAILIAGILWRAWLYQQGMTDEAITRAEWLLLGAMLLIAG
                     VITFVLHPQAAALQAQAFASKGEDARKAAFEAFFQLHKPVRVLYILNVGLGIALLTLR
                     VRSWIRVKE"
     gene            complement(3148..4437)
                     /locus_tag="PK589_17475"
     CDS             complement(3148..4437)
                     /locus_tag="PK589_17475"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNJ21456.1"
                     /translation="MILIKGGHVIDPGRVNGIADVLIENGKIAAVGQKLAAPAGATVI
                     QAGGQLVLPGFVDLHVHFREPGFEYKETIQSGTDAAVAGGFTTVCAMPNTNPVNDNQA
                     VTEFMLERAKAAGTANLYPIGAITKRSEGKELAEIGDLRRAGCVAISDDGKPVMNSLV
                     MRRAMEYARAFDVPVVDHCEDLHLSEGGCMNEGLISTELGLPGIPSAAEDVMVARNVS
                     LAELTGARLHLAHISTAGSVRMVREAKARGLKVTAEACPHHFTLTEETTRGYNTHAKM
                     NPPLRTSQDVQAIKDGLRDGTIDVIATDHAPHASQEKQQEFTEAPFGIVGLETALPLT
                     LTLVEEGVLTLESAVDKLATAPAKAFSLNAGTLAVGAPADVAIVDPNLEWEVDPSRFR
                     SKSRNTPFAGWKVKGRVTTTLVSGRVVYELDRPGRQA"
     gene            complement(4527..5450)
                     /locus_tag="PK589_17480"
     CDS             complement(4527..5450)
                     /locus_tag="PK589_17480"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNJ21457.1"
                     /translation="MGLKRKDLLSLTNLSAADIALILDTADSFKEVSGREIKKVPALR
                     GKTVVNLFFEPSTRTRTSFELAAKRLSADVINFSPSSSSVVKGETLLDTARNIEAMQA
                     DIIVLRHSSAGAAETLARGVKSSVINAGDGWHEHPTQALLDLYTIRSRGMGFSGLKVA
                     IVGDVAHSRVARSNIFALTKLGAEVRVVGPPTMIPLHINQLGAQVYYNLDEGLRGVHV
                     IMMLRLQLERQGRALFPTIREYARQYGLTNERVKLAEPGAIVMHPGPINRGVEIAPDV
                     ADSLSSVILDQVANGVAVRMGILYLMSGANA"
     gene            complement(5465..6049)
                     /gene="pyrR"
                     /locus_tag="PK589_17485"
     CDS             complement(5465..6049)
                     /gene="pyrR"
                     /locus_tag="PK589_17485"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNJ21458.1"
                     /translation="MPPADRPLMTTEPTTGNRQERVVMDAGDIARAVTRIAHEILERN
                     KGIQDLALVGIRTGGVHLAHRLVRRIHDIEGTQIPIGELDITLYRDDLSLRKDQPILR
                     TTSVPFKISDLKVVLVDDVLFTGRTIRAAMDGLMDLGRPAEIQLAVLVDRGHRQLPIK
                     ANYIGKNIPTSRDEAIEVHLEENGEEDRVVILRT"
     gene            6358..>7627
                     /locus_tag="PK589_17490"
     CDS             6358..>7627
                     /locus_tag="PK589_17490"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNJ21459.1"
                     /translation="MTSPSGSTTTPDLLTALANTATNLRIDSVKATSEAGSGHPSSCC
                     SAADILAVLFFSVMRYDPKNPKLPNSDRFVLSKGHAAPLLYAAWAEAGLFPKSELLKL
                     RTLGSDLEGHPTPRLSFVDMATGSLGQGLPAGVGLAFNAKSIDKTDYRTYVLMGDGES
                     VEGSVWEAAEVARQAKLDNLCAIVDVNRLGQSDPTMLQHDMDAYRARWSGFGWHAIVV
                     DGHDISALVAAFAEAAKTKGQPTVLLAKTFKGRGISFMENHPDWHGKPLKKGEETQKA
                     LDELTGQLKPSSTRLQISLPTAAKTAPSTKGTLAPAPYKVGDSVATREAFGAALLALG
                     EANSQVVALDADVKNSTYSDKFGKRFPDRFLENFIAEQNMLGAAAGIAACGKIPFVAT
                     FAAFFTRAYDFIRMAAISQSNIKLVGTHVGV"
BASE COUNT         1462 a         2335 c         2267 g         1563 t
ORIGIN      
        1 cccggaaggc cgagagaccc atcgccattt ctttttcttc cgacagataa atgaactggt
       61 cgcgcgccgt ccccggtgcc cgttgacacc ctccgagcga ttgcagcagt cccgtcgcgg
      121 ctccaagccc catggtcgtg gccaacagcg aagcggcgcg cgcacccagt gcgagggcgg
      181 tacgtcgccc gatcggcgtc ttcagcagcg tttcgattga atgtcggttt gacggataca
      241 tcacgtctgc tccattcaat cagccccgcg cggcggaagc aactcaccgg cttacggcat
      301 ccacagatac atgaattgcg gtgacccgcc tcgcacgacc gacagcagat cgacgttcgc
      361 cagactggcc aggctgggcg ccggcgcgac gagccgcgaa tagacattgt tctggtattc
      421 gccgggccgt cgataggtca cgaccttcgc gtccgtcaag ccggcttttt tcttggccag
      481 ttcaatcgcg tcgtcgaggt agccgatatc atcgaccaat cccgcagcct tggcctgttc
      541 gccggaatag atccggccgt cggcgagctt cttaatggtc tccccgttca tgcccgggcg
      601 gccttcctgg accacctgca gaaaccgctg ataaaagccg tcgatcacgc cttggaaaat
      661 cgcccgttct tccggcagca tcgcgcgaaa cggcgagccc atgtccttgc ggggaccgga
      721 ggtgacggca ttcgtttcga cgccgacttt ttcgagcagg cctctggcat tgacggtcag
      781 catgatcacg ccgatactgc cggtgaccga tgagggatgc gccagcaccg tatcggctgc
      841 ggcggcgatg tagtatccgc ctgacgcgcc cacgtccatg atggaggcca caataggaat
      901 tttgcggctg gtcttaaagt ttctgagttc gtgataaatg atgtcggacg cggtgacagt
      961 ccctcccggg ctgttgatgc gcagcacgat cgctttgacc cgttcgtctt tggcggcccg
     1021 ctccagctct tccttcaccg tggccagcat gccaggcgaa gaataaaagc cgtccttgtt
     1081 ttcggagctg atcacgcccg acacatccat gagcaggacc ttgtctttgc cggtcccgct
     1141 gaccttgtgt tcttcgagcg cgccggggcc cggcggcagg ttgatcgtga cgcaggccgc
     1201 ctgcatcagg gccagggcca acacaagcag accggtcgag agctgccata cgtcgggagc
     1261 ctgaccgacg ttgcgcttca tagcgaggcg aaggagctgc ggccagatgc gaggccggag
     1321 gcctgaaaaa accggaagtg tattcgcaga attacattga ggatttttga gggccgagaa
     1381 cgacgcagat ggccgcaggt cattcgccgc agcagagcgg tcaatatcgg acaggctcct
     1441 aggcatggtg tgtctccatc aatgcgacaa attgttcaaa cagataggcg gaatcatgag
     1501 gaccgggaga cgcctcggga tggtattgca ccgaaaacac cgggcgatcc agacaccgca
     1561 tgccttccac ggatgcgtca ttcaagctgg tgtgggtcaa ttgcacccga ccgaacggcg
     1621 tatccacgat cggcagttcc tgacccacaa ccggagtttc gccggggaac cgcacggcaa
     1681 agttgtgatt ctgagaggtg atttcgacct ttctggtgcg caggtcgatg accggatgat
     1741 tggcgccatg gtggccgaac ttcagtttgt acgtggagaa tcccaacgcg agccccagca
     1801 attgatgccc caggcagatg ccgaaaatcg gcacgcggcc gatgagctca cggagtgcgg
     1861 tcatggcgta gggcacacct tccgggtcac cgggaccatt cgagaggaac aatccttgcg
     1921 gattcagggc caacacatcc tttgccggag tcgaggccgg caccacggtg acctcgcacc
     1981 ccacatccac cagccgccgc agaatgttct gtttcacacc gaaatcatag gcaacgacgc
     2041 gccaggtttt gcgcggtgcc ttcgcggccc cctgttccgg catcgtgatc ttcggcgccc
     2101 aatcacccgt gccttccgtc caggggtaac gatgttcgca ggtgacggtg gctgccagat
     2161 cacgcccgat gatgctcggc gcctgccggg ccttctcggc cacgcggcgg gggtcgagat
     2221 cgatgtggga aatgacgccc ggttgggcgc cccgctcccg caaatgcctg gtgagcgctc
     2281 tggtatcgat gccctcgatc gccacgatat gagcggcctg cagatactct tgcagcgtcg
     2341 acttgcctcg ccagttactc gccagccggc tggattcctt cacgatgaac ccttcggccc
     2401 agattttcgt cgactcgacg tcctccggcg tgacgccgta gttgccgatc tgtggagacg
     2461 tcatggtgac gatctgcccg cgatacgaag gatcggtcag aacttcctga tagccggtca
     2521 tagccgtgtt gaagacgact tcgccgccgg tctccccttc cgcgcctaaa gctcgccctt
     2581 cgaaccaggt tccatcggca agcgcaagaa tcgctttctt cactctcact ccttcactcg
     2641 gatccaggac cgcacgcgca gggtcaacag cgcgataccg agtcccacat tcaggatata
     2701 cagcacccgt accggtttat gtagctgaaa gaacgcctca aaggctgcct tcctcgcgtc
     2761 ctcccctttg gaggcaaagg cctgcgcctg caaggcggcc gcctgaggat gcagcacaaa
     2821 cgtgatcaca ccggcaatga gcagcattgc gcccagcaac aaccactcgg ctctggtgat
     2881 ggcttcgtcc gtcattccct gttgatacaa ccaggctcgc cagagaatgc ctgcgatcag
     2941 aatggccgcg gcgccaagca ccagccgatt atacccgtcg aacgcgcgcg tcaggaaaaa
     3001 tcccccggtg tcctgtccgc cgaacgtatt gaacacggcg gggatgacag ctgccacgag
     3061 gacgagaagt ccgccgatcc agacggctaa tgccagcatc tcgaaggtca cacaggcgat
     3121 caatccttga cgcacgatcg tctcgtccta cgcctgccgt cctgggcgat cgagttcgta
     3181 gacgacccgc ccggagacga gtgtcgtggt gacacgccct ttgactttcc atccggcgaa
     3241 cggcgtgttc cggctcttgg aacgaaatcg cgagggatcg acttcccatt ccagattggg
     3301 atcgacgatg gccacatccg ccggcgcgcc gaccgccagg gtgccggcat tgagactgaa
     3361 cgccttcgcc ggagccgtgg ccagcttatc gacggccgat tcgagcgtga gcaccccttc
     3421 ttcgaccagg gtcagcgtca gcggcaacgc ggtttccagt cctacgatgc cgaacggcgc
     3481 ctcggtgaac tcctgttgtt tctcctggct ggcgtgcggc gcatggtcgg tcgcaatgac
     3541 gtcgatggtg ccatcacgta atccgtcctt gatcgcctgc acatcctgac tggtcctcag
     3601 tggcggattc attttcgcat gggtgttgta gccgcgggtc gtttcctccg tgagggtaaa
     3661 gtgatggggg caggcttctg ccgtcacttt gagcccccgc gccttggctt cgcgcaccat
     3721 ccggaccgaa ccggccgtgc tgatgtgcgc cagatggaga cgcgctccgg tcagttcggc
     3781 gagcgagaca ttgcgcgcca ccatcacatc ttccgcggcc gacggaatgc ccgggagtcc
     3841 cagttcggtc gagatcagcc cttcattcat gcagccgcct tcggagagat gcaaatcctc
     3901 gcagtggtcg acgaccggta cgtcgaaggc gcgcgcatat tccatcgcgc ggcgcatgac
     3961 gaggctgttc atcaccggct ttccgtcgtc ggaaatcgcg acacatccgg cgcgacgcag
     4021 gtcgccgatc tccgccaatt ccttgccttc ggagcgtttg gtaatggcgc cgatgggata
     4081 gaggttggcg gtgcctgccg ctttcgcccg ctccagcatg aattcggtga ccgcttggtt
     4141 gtcgttcacg ggattggtgt tgggcattgc gcagactgtg gtgaaacctc ccgcaacggc
     4201 cgcgtccgtt cctgactgaa tcgtttcctt gtattcaaag cccggttcgc gaaagtgcac
     4261 gtggagatcc acgaagcccg ggagcaccaa ctgcccccca gcctgaatca ccgtcgcgcc
     4321 ggccggtgcg gcgagctttt gcccaaccgc tgcaattttc ccgttttcaa tgaggacatc
     4381 cgcgatgccg ttgacgcgtc cgggatcgat aacgtgaccg cccttgatca gaatcatagt
     4441 gcgctgttca actcccccta ttaaacaatg taaaccatgg tttcatgtgc cggcgatctc
     4501 ctgtgaggag atcgtctcat cacgtcttac gcattggctc ccgacatgag atacaaaatg
     4561 cccatacgca ccgccacgcc gttggccact tgatcgagga tcacggacga caggctgtct
     4621 gctacgtccg gtgcgatttc gacgccccga ttgatggggc ccggatgcat gacgatcgcg
     4681 cccggctcag ccagtttgac ccgctcgttg gttaagccgt actgccgggc atattcgcga
     4741 atggtcggga acaaggcgcg cccctggcgt tcgagttgaa gccgcagcat catgatcaca
     4801 tgcacgcccc gcaacccttc atccaggttg taatacacct gggcgccgag ctggttgatg
     4861 tgtagcggaa tcatcgtcgg tggtccgacg acccgcactt cggctccgag tttcgtgagg
     4921 gcgaaaatat tggaacgggc cacgcggctg tgtgcgacgt cgccgacaat cgccaccttg
     4981 agcccagaga accccatgcc gcggctgcgg atggtgtaga gatcgagcag cgcctgagtc
     5041 ggatgttcgt gccacccgtc gccggcattg atgacggaag acttgacgcc tcgcgccagt
     5101 gtttccgccg cgcctgccga tgaatgccgc aacacgatga tatccgcctg catggcttcg
     5161 atattgcgcg cggtatccag cagcgtttcg cccttcacca cactgctgga cgaaggggaa
     5221 aaattgatga catcggcgct gagccgcttg gcggccaatt caaacgaggt tctggttctc
     5281 gtgctcggct cgaaaaagag gttcacgacc gtcttgcccc gcaaggccgg caccttcttg
     5341 atctcccgcc cggagacttc tttgaacgaa tccgccgtgt ccaggatcag ggcaatgtcg
     5401 gcagctgaca aattggtcag gctgagcagg tctttgcgtt tgagccccac gtcactgtct
     5461 ccggttacgt cctgaggatg accacgcggt cctcttcccc gttttcttcg agatggacct
     5521 caatggcctc gtcgcgcgag gtgggaatgt ttttcccgat atagttggct ttgatcggga
     5581 gttgccggtg accgcgatcg accagcaccg ccagctggat ttccgccggc cgtcccagat
     5641 ccatcagtcc gtccatggcg gcacggatgg tccggcctgt gaagagcaca tcgtcgacga
     5701 gcacgacctt caggtcagat attttgaacg gcaccgaggt ggtgcggagg atcggttgat
     5761 ccttccgcaa cgagaggtcg tcccggtaca gcgtgatatc cagttcgccg atcgggatct
     5821 gggtgccttc aatgtcatgg atgcgccgca cgagtcgatg cgccaggtgg acgccgcccg
     5881 ttctgatgcc gacgagggcg agatcttgaa tgcctttatt ccgttccagg atttcgtggg
     5941 caatgcgggt cacggcgcgg gcaatatcgc ccgcgtccat cacgacgcgc tcctgccggt
     6001 tgccggttgt aggttccgtg gtcatgagtg gccgatcagc tggaggcata aaaaaacctt
     6061 ctcctccggt gtggacgaga aggtgcgggt tgtcggacgg cttgagccgt gcatcatgat
     6121 catgagctcc ttacccacct cacaggatga gcgttaaagg cgagccgatc ttaacgagtt
     6181 gatccaaccg tgtcaagaat tcactgcagg cgatgggctg atgagaggga catgccttca
     6241 ttgactccat gcatgccatt tgttagcttc gtcccaccga aagcggactc tcgctctcga
     6301 tctctttgtc aatcaggtca tccagaatca tcagctccca tcgtgaaagg atcgcgcatg
     6361 acttcccctt ctggttccac cacaactcct gatctgttga ccgccctcgc caatacagcg
     6421 accaacctcc gcatcgacag cgtcaaagca acgagcgaag cagggagcgg acatccgtcc
     6481 agctgttgct cggccgcgga cattcttgcg gtgttgttct tctcggtcat gcggtacgac
     6541 cccaagaatc cgaagttgcc gaatagcgac cggtttgtcc tctccaaggg gcacgccgct
     6601 cctctgctct atgcggcctg ggcggaagcc ggattatttc ccaagtcgga gctgttgaaa
     6661 ttgcgcacgc tcggctccga tcttgaaggc catccgacgc cgcgactctc gttcgtcgac
     6721 atggcgaccg gttcattggg ccaggggctg ccggcgggtg tcggtctcgc gttcaatgcg
     6781 aagtccatcg acaagaccga ctaccgcacc tatgtcctga tgggcgacgg cgaatctgtc
     6841 gaggggtctg tgtgggaagc cgcggaagtg gctcgccaag cgaagctgga taatctgtgc
     6901 gccatcgtgg acgtgaatcg gttgggacaa agcgatccga cgatgctgca gcatgatatg
     6961 gacgcctacc gcgcccggtg gagcgggttc gggtggcatg cgatcgtggt ggacggtcac
     7021 gatatcagcg ccctggtcgc ggcattcgcc gaggcggcga agacgaaggg gcagccgacg
     7081 gtcttgctgg caaagacctt caaggggcgc ggcatttcct ttatggaaaa tcatccggac
     7141 tggcacggga agccgctgaa gaagggcgaa gaaacccaaa aggcgttgga cgaattgacg
     7201 ggccaattga aaccgagctc gacccgcctt cagatctcgt tgccgactgc agccaagacg
     7261 gctccgtcca cgaagggcac gctggctccg gcaccgtaca aggtgggcga ttcggtggcg
     7321 acgcgggaag ccttcggggc cgccctcctg gcgcttggcg aggcgaactc tcaggtggtc
     7381 gcgctcgatg cagacgtgaa gaactctacg tacagtgata agttcggtaa acggtttcca
     7441 gaccggttcc tcgaaaactt tatcgccgag cagaatatgc ttggcgctgc ggccggcatt
     7501 gcggcctgcg gcaagattcc gttcgtggcg accttcgcgg cgtttttcac gcgcgcctat
     7561 gattttattc gcatggcggc gatcagccag tcgaacatta aactggtggg gactcacgtc
     7621 ggtgtca
//
LOCUS       DAOQDS010000109         7610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_5468_length_7610_cov_19.090536, whole genome shotgun sequence.
ACCESSION   DAOQDS010000109 DAOQDS010000000
VERSION     DAOQDS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5468_length_7610_cov_19.090536"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PK589_17495"
     CDS             <1..672
                     /locus_tag="PK589_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ21460.1"
                     /translation="MAAISQSNIKLVGTHVGVSIGEDGPSQMGLEDLAMMSTQPGVTV
                     LYPSDGLSMYKLMESATAHKGMVYLRAGRPKTPVIYSADETFKIGGSKILRQSAADQL
                     TIVAAGVTLFEALKAHDQLKAAGIATRVVDLYSIVPVDQATLIDCARATGGRFLTVED
                     HYAHGGIGDAVLSALASEGVRVRKLAVRDIPRSGKPDELVDHFGIGVRSIVEAAKEIA
                     TAARR"
     gene            629..1369
                     /locus_tag="PK589_17500"
     CDS             629..1369
                     /locus_tag="PK589_17500"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNJ21461.1"
                     /translation="MSKQPKRSPQQPAAERPSPGAPAGLNQIPLLQILSAQDRQKVLG
                     EMTETRYGKGDYIFREGDPTEYFHIVKEGSVKCVKSSLDGKECTLKVLMPGDLFCCDA
                     SAFDGASHPGTAQPMGDVSVLRMKKEAYFKMLRANPDAAMEVIRYLGNRLNEAQEKAK
                     VLALDRADQRLASLLVSLAERSGIKDPNGIRVTVRLTRQDMANMVGVTTETAIRIMAR
                     FKKDRLVTGTAARLVIRDLPKLKLLAST"
     assembly_gap    1450..1549
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1550..2374
                     /locus_tag="PK589_17505"
     CDS             <1550..2374
                     /locus_tag="PK589_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNJ21462.1"
                     /translation="DIVIDGGGEKYAAWTFNGQFPGPVVRVTEGDTINFTLTNPATNK
                     NPHAMDFHAAEIDFLKNYRAVNTGESISFTFVAKKPGLFFYHCGAPPMIQHVARGMFG
                     AIIVDPKNAKVWPKADREYVLVQSEYYKNPNDVQAMFDRKYDGMMFNGGIFKYHPFVT
                     GGGKLDAKPGERVRIYFVNAGPNEFSSFHPIGEIWDNVYESGNPANNLKGVQTYVVGP
                     GSAATFDVVVESAGAYPLVTHSLTGALRGAIAVLLAGPDAKPAPLMPMVPWEIPAK"
     gene            2487..2834
                     /locus_tag="PK589_17510"
     CDS             2487..2834
                     /locus_tag="PK589_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21463.1"
                     /translation="MRCPGMTLSCLLLFAGCLVSPASADTSRERVALFLSGQDCPSSR
                     RNIAAELAQVAGVIRVDMESVPDHALVDVVIGAVSPDDLLVAVHRSALKGVDCRAEVM
                     KSCISAGRSAADR"
     gene            2896..3525
                     /locus_tag="PK589_17515"
     CDS             2896..3525
                     /locus_tag="PK589_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21464.1"
                     /translation="MRTTVLKGQRGIAWPFWLAVMATVWLLPVAPVVAQEAVLEFPIR
                     PFNVDVMLRQQTFAPDDREIGVQLGITAVRYGNVEVRTIYQFFSIHTQDFSTDQHSLF
                     VNPRWNNFIDILDFPKHKPISRIIRHVLFGPLEDRAVPYVGVLAGGVLPGPGNRAPGH
                     LIGGQVGVRFPVAQSLSVDISVQYSQYGVDFRGDAGQAQQWVFLTGIRF"
     gene            3495..4556
                     /locus_tag="PK589_17520"
     CDS             3495..4556
                     /locus_tag="PK589_17520"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNJ21465.1"
                     /translation="MGIPDRDTVLIKATWCGRIWAALCCLLLSGCGLLIDAVEQVWPV
                     AGSKVDIICERGRVQVGLAMEPFRPFVFPTIWTDEGARVTGLDVELTRAMTDELSRRC
                     GHPVTPVLHLVRFRDLFRLLNEGHLDWFVSAVATNTPAPSRAGVAYSLPYFYGGGISG
                     ITTSTSVLERVRENLQQQATHPAADRLAATSHALDGLTIAVQDETSAYYYAEANWGAN
                     RLIVCDSLPAAFEYADAKTEPRVDIILGAQPVLEYMVKRVRKDWSLLTRENGRPLFLT
                     KADYGVVVAEESYRLRWLVNDLLLKLDESGRLGEMRTRWLDDEYAFPRRASLEGLPFD
                     VEKMASHYIQGTCRIRPGS"
     gene            4579..5337
                     /locus_tag="PK589_17525"
     CDS             4579..5337
                     /locus_tag="PK589_17525"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNJ21466.1"
                     /translation="MRRRAWIALAAVMLLMCLHPSMSVAVERAEAEETARLLAKLLES
                     GRAVIERNQSLIDDPHKGEKGFTPELFEQQLIREFHAKTGIDLNALPAAPVSLRIPPL
                     AKELLPALVQASREVVRDAQVVINQRGIGYKNFIPATYGSQASARFSKAAHVRLKQTT
                     IQPRNPKNEPDEYEASVLKWLSARPRAEAYVSELTDGGRTLRVVMPIYYAKDCLACHG
                     EPKGDLDISGYPKEGHQEGDLAGAITVTAPLGNR"
     gene            complement(5340..5657)
                     /locus_tag="PK589_17530"
     CDS             complement(5340..5657)
                     /locus_tag="PK589_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNJ21467.1"
                     /translation="MKVIALSDFQQFSQEKMKKNNLFETSRFFCDIYCFEPGQEQKGH
                     IHGEQDKVYIVLEGEGTFQVGTEKRVLKAGQGTMAAAGEEHGVKNHSAERLKVLVFVA
                     PNP"
     gene            complement(5654..6577)
                     /locus_tag="PK589_17535"
     CDS             complement(5654..6577)
                     /locus_tag="PK589_17535"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HNJ21468.1"
                     /translation="MPLKFALYPGCAAKGATPELYQSTMAIVGRLGIEVVELAASSCC
                     GAGVIGEADPDLALALNARTFAQAERMGLDVMTICGTCQGVMSAANRRLKSEPATLER
                     VNKILAQDGISYGGGVQVKHLLWIAVRDLGLSRVAGTVRSSFREFRIAPFYGCYMLRP
                     SWELGFDDPENPSSLEKIIRAVGGEPVAYAGRTKCCGFPIILEKEAIAVAMAGTNMKD
                     AKDHGADFMVTPCPLCHMSLDIYQERAGRAVKTELNLPILHLPQLLGLAMGIPAQDLG
                     LSRHLISVDRIVNRLQNPDSTHSSLPAEPRT"
     gene            complement(6702..7148)
                     /locus_tag="PK589_17540"
     CDS             complement(6702..7148)
                     /locus_tag="PK589_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21469.1"
                     /translation="MIAERVLVVLCLTAVGCGLAPLAIAAGDQAPPSTTEKMIRDTKE
                     AVESTKQYTIQQKDSFQKTVQAELAEMQTKITELQKKTSAASVEARKDMQKALQDLER
                     KKDEARKQLDEVSHSTSSAWSTLKDNVNAAVADLKKSYKDTVSKLP"
     gene            7340..>7610
                     /locus_tag="PK589_17545"
     CDS             7340..>7610
                     /locus_tag="PK589_17545"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNJ21470.1"
                     /translation="MSAELAPMSDPASTSPLEQLLIERIRKGAIELPLLPQVASRILA
                     MVYDPDAEAAKLAALIHQDQALAAHVIRIANSPAYMTRNPVVSLQH"
BASE COUNT         1496 a         2172 c         2263 g         1579 t
ORIGIN      
        1 atggcggcga tcagccagtc gaacattaaa ctggtgggga ctcacgtcgg tgtcagcatc
       61 ggtgaggacg gcccgtcgca gatgggactt gaagatctcg ccatgatgtc gacccagccc
      121 ggcgtgaccg tgctctaccc gtccgatggc ctgtcgatgt acaaattgat ggaatcggcg
      181 accgcccaca aggggatggt gtatctacgg gccggtcgtc ccaagacccc cgtgatctat
      241 agtgcggacg agacgttcaa gatcggcggt tcaaagattc tgcgacagag tgccgccgac
      301 cagctcacga tcgtcgccgc cggtgtcacg ctgttcgaag ccctcaaggc ccacgaccag
      361 ctgaaggcag ccggcatcgc cacccgcgtg gtagatttat acagcatcgt gcccgttgac
      421 caggccacct tgatcgactg tgcgcgagcg acgggtggac ggtttctgac ggtcgaagat
      481 cattatgcgc acggcgggat cggcgacgct gtgttgagcg ccctggcatc agaaggcgta
      541 cgggtgcgca agttggccgt ccgggacatt ccacgtagcg gaaaaccgga cgagctcgtg
      601 gatcattttg gaattggagt gcgctcgatt gtcgaagcag ccaaagagat cgccacagca
      661 gcccgccgct gaacggccca gccccggtgc tcctgccggg ttgaatcaga ttcccctgct
      721 ccagatcctc agcgcccagg atcgccaaaa ggtcctgggc gaaatgactg aaacccgcta
      781 cgggaagggc gactacattt tccgcgaagg cgatccgacc gaatacttcc acatcgtcaa
      841 ggaagggtcg gtcaaatgcg tcaagtcgtc gctcgacggg aaggaatgca cgctcaaagt
      901 cttgatgccg ggtgatttgt tttgttgcga tgcctcggcc ttcgacggtg cgtcccaccc
      961 tggcacggcc cagcccatgg gcgatgtcag tgtgcttcgc atgaaaaagg aagcctactt
     1021 caagatgctc cgggcgaatc cggatgccgc gatggaagtc atccggtatt tgggaaaccg
     1081 gctcaatgaa gcccaggaaa aagccaaagt gcttgccctc gatcgagccg atcaacgatt
     1141 ggcttctttg ctcgtgagtc tggcagaacg cagcgggatc aaggacccca acgggattcg
     1201 ggtcaccgtt cgtctgacgc gccaggacat ggccaacatg gtgggcgtca cgacggaaac
     1261 cgccatccgg atcatggcgc gcttcaaaaa ggatcgattg gtgaccggca ccgccgcgcg
     1321 gctcgtcatt cgtgatctcc ccaagctcaa actcctcgct tccacgtaat ttctttcgcc
     1381 acagaccggg tatttttaaa aacatgacaa tagtcatgtt ttgttgatgc cgccatctcg
     1441 tatggtgcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng acattgtgat
     1561 cgacggcggc ggtgaaaaat atgccgcctg gacgttcaac gggcagtttc cggggccggt
     1621 cgtgcgggtg acggaaggtg acacgatcaa tttcaccctg acgaatcctg cgacgaacaa
     1681 gaatccgcat gcgatggact tccacgcggc tgaaatcgat ttcctgaaaa attatcgggc
     1741 cgtcaatacc ggcgaaagca tcagcttcac ctttgtggcg aaaaagccgg gactgttttt
     1801 ctaccattgc ggagcgccgc ctatgattca acatgtcgcg cgcggcatgt tcggcgcgat
     1861 catcgtcgat cccaagaatg ctaaagtctg gccgaaagcg gatcgggaat atgtgctggt
     1921 gcagtcggag tattataaga acccgaacga tgtccaggcc atgttcgatc gaaagtacga
     1981 cggcatgatg ttcaacggcg gcatcttcaa gtatcacccc ttcgtgaccg gcgggggaaa
     2041 actcgatgcc aaaccgggag agcgcgtccg catctatttc gtcaacgcgg ggccgaacga
     2101 gttctcctcg ttccacccga tcggtgagat ctgggacaac gtgtacgaga gtggcaatcc
     2161 tgccaacaat ctgaagggcg tgcagacgta cgtcgtaggg cccggcagtg ccgccacgtt
     2221 tgatgtcgtg gtggaatctg ccggagccta tccgctcgtc acgcattcct taaccggggc
     2281 attgcgtggg gcgattgcgg tgttgctggc gggaccggat gcgaaaccgg cgccgttgat
     2341 gccgatggtc ccgtgggaga tcccagcgaa gtagaccgtt tcgtgatcga atcttcggcc
     2401 ggcccctaag gggtcggccg gacaacgcga gagatcaaca atgacgacac cgtttatgca
     2461 tggatcgtgg tttcttggag atgccgatgc gatgtcctgg gatgacgctg agttgcttac
     2521 tcctgttcgc cgggtgcctc gtgagtccag ccagtgccga cacctcgcgc gagcgagtgg
     2581 cgctcttctt atccggccaa gattgtccct cttcccgccg gaatatcgct gcggaactgg
     2641 cgcaagtagc aggcgtcatc cgcgtggaca tggagagcgt tcctgaccat gcgctggtcg
     2701 atgtcgtgat cggtgcggtg agtccggacg atcttctggt ggcggtgcac cgctccgccc
     2761 tcaagggcgt ggattgtcgg gcggaggtca tgaagtcatg catttccgcc ggtcgctctg
     2821 cagctgatcg gtgaccagca cgtgccacga ctggttgatc gaggcgaccg ggccacgctc
     2881 acgcgaaggt ctgccatgcg gacaacggtc ttaaaggggc aacgagggat cgcctggccc
     2941 ttttggctgg ctgtgatggc cacagtctgg ctgttgcctg tggcgccggt ggtcgctcag
     3001 gaagcggtcc ttgaatttcc cattcggcct tttaatgtcg atgtgatgct ccgccagcag
     3061 acgtttgcgc cagacgatcg tgagatcggc gtgcagctcg gcattacggc ggtacgctac
     3121 ggcaatgtcg aggttcggac catctatcaa ttcttcagca tccatacgca ggacttcagc
     3181 accgaccagc attcgctgtt cgtgaatcct cgctggaata acttcatcga catcctggat
     3241 tttcccaagc acaaaccgat cagccgcatc atccgccatg tgctgtttgg accgttggag
     3301 gatcgggccg ttccctatgt gggagtattg gccggaggtg tcttgcccgg ccctggtaac
     3361 cgtgctccag gccatttgat cggcggccag gtgggagtgc ggttcccggt cgcacagagc
     3421 ctctcagtgg atatcagcgt gcaatacagt caatacgggg tggattttcg cggagacgcg
     3481 ggacaggcgc agcaatgggt attcctgaca gggatacggt tttaatcaag gcaacctggt
     3541 gcggccgaat ctgggcagca ctatgctgtc tgctgttgtc cggctgcggc ttgctcatcg
     3601 atgcggttga acaggtctgg ccggtcgccg gcagtaaagt cgatatcatc tgcgaacgcg
     3661 ggcgtgttca ggtcggcctg gcgatggagc cgttccgtcc cttcgtgttt ccgacgatct
     3721 ggacggacga aggggctcgt gtcacggggc tcgatgtcga actcacccgg gccatgacgg
     3781 acgaactgtc ccgacggtgc ggacatccgg tgactcccgt cctgcatctc gtgcgcttca
     3841 gggatctgtt ccggctcctg aatgaaggac acttggattg gttcgtgtcg gctgtcgcga
     3901 caaacacgcc tgccccgtcc cgcgccggag tcgcctattc cctgccctat ttttacgggg
     3961 gaggtatcag cgggatcaca acgagcacga gcgtcctgga gcgggtgcgg gagaatcttc
     4021 agcaacaggc gacgcatcct gcagccgatc ggctcgcggc cacgagtcat gcgctggatg
     4081 gcctcaccat cgcggtgcag gatgaaacca gcgcgtacta ctatgccgaa gccaactggg
     4141 gcgccaaccg gctgatcgtg tgcgattcgt taccggcagc cttcgaatat gccgacgcca
     4201 agaccgagcc gcgtgtcgat attattctgg gcgcacaacc ggtgttggaa tatatggtca
     4261 agcgggttcg caaggactgg tcactcttga cccgtgagaa cggccgcccc ctgttcctga
     4321 ccaaggccga ctacggagtg gttgtggctg aggagagtta ccgcctccgc tggctggtga
     4381 acgatctact gttgaaactg gacgaatccg gtcgcctggg tgagatgcgg acacgttggc
     4441 tcgacgacga gtatgcgttt ccccggagag cctcgttgga aggattaccc ttcgatgtgg
     4501 agaagatggc gtcccactac atccagggca cctgccgcat caggcccgga tcatgaccgc
     4561 ctggagagga gcacaatcat gagacgtcgt gcatggatcg ccctggctgc agtgatgctg
     4621 ctgatgtgcc tgcatccttc gatgagcgta gcggtggagc gagccgaggc ggaggaaacc
     4681 gcgcgcctgc tggccaaact gttggaatca gggcgggccg tcatcgaacg caaccagtcg
     4741 ttgatcgacg atcctcacaa gggcgaaaag ggattcacac cggagttatt cgagcagcaa
     4801 ttgatccggg agtttcacgc caagaccggg attgacctga acgcgctgcc ggccgcgccg
     4861 gtgtctctac gcattccacc gctggcgaag gagctgctgc cagccttggt ccaggccagc
     4921 cgcgaggtcg tccgtgatgc ccaggtcgtc atcaatcagc gcggcatcgg ctacaagaac
     4981 tttattccgg ccacatacgg cagtcaggcc tctgcgcggt tctccaaggc cgcgcatgtg
     5041 cggctgaagc agaccactat ccaaccgcgg aatccgaaaa acgagcccga cgagtacgaa
     5101 gcgtcggtgt tgaaatggct ctccgccagg cccagggccg aggcctatgt gagcgaattg
     5161 accgacggag ggcgtaccct acgggtagtc atgccgattt attacgccaa ggattgtctg
     5221 gcctgccatg gagaaccgaa aggtgatctc gacatttccg ggtatcccaa agaagggcat
     5281 caggagggag atcttgccgg agcgattaca gtcaccgccc cgctggggaa tcgataacct
     5341 cagggatttg gcgcgacaaa cacgagcacc ttcaaacgct ccgcgctgtg attcttgacg
     5401 ccgtgctcct cacccgcagc ggccatcgtc ccctgtccgg ctttcaggac acgtttttcc
     5461 gtgcccacct gaaaggtgcc ctcgccttcc aacacgatgt acaccttgtc ctgttcgccg
     5521 tgaatatgcc ccttctgttc ctgtcccggt tcgaagcaat agatgtcgca aaaaaaccgc
     5581 gacgtttcga acaggttatt cttcttcatt ttctcttggc tgaactgctg aaaatccgaa
     5641 agggcgatga ccttcatgtc ctgggctccg cgggtaaaga tgaatgggta ctgtccggat
     5701 tctgcaaacg attgacgata cgatcgactg aaatcagatg gcgcgacagg cccaggtctt
     5761 gcgcgggaat acccatggcc agaccgagca gttgcgggag atgcagaatc gggaggttca
     5821 gttcggtctt caccgcccgc cctgcgcgct cctgatagat atccaggctc atgtgacaga
     5881 gcggacaggg cgtcaccatg aaatccgcgc cgtgatcctt cgcatctttc atattcgtgc
     5941 cggccatggc caccgcgatg gcctccttct ccaggataat cgggaagccg cagcatttgg
     6001 tccggcctgc gtaggcgacc ggctctccac cgacggcgcg aatgattttt tccagcgaac
     6061 tgggattttc gggatcatcg aatcccagtt cccaggaggg gcgaagcatg tagcagccgt
     6121 aaaacggagc gatgcgaaac tcgcgaaatg atgagcggac agttccggcc acgcgactga
     6181 gcccgagatc gcgtaccgcg atccagagaa gatgtttcac ctgcacgccg ccgccgtagg
     6241 agatgccatc ctgtgcgagg atcttgttca cccgttcaag cgtggccggt tcgcttttca
     6301 gccgccgatt ggctgcgctc atcacgccct ggcaggtccc gcaaatggtc atcacgtcca
     6361 gccccatccg ctcggcctgc gcgaaggtgc gggcgttcaa ggcgagcgcg aggtccgggt
     6421 ctgcttcccc gatcacccct gctccgcaac aggacgacgc cgcgagttcg acaacctcga
     6481 tgccgaggcg gccgacaatg gccatggtgg attggtagag ttcaggcgtc gcgcctttcg
     6541 cggcgcaacc gggatagagg gcaaatttaa gcggcatggg cctcgtcgag tagttgtcct
     6601 gcttgtcctt gcaccttacc gtattttccc cctcgatgaa cagctcgggc ggtgattcat
     6661 tgacggggaa ccccgcgtgt tgagaaagtc ggctgtccag gtcagggcag tttggacacc
     6721 gtgtccttat aacttttttt gagatcggcc acggccgcgt tgacgttgtc tttcagcgtg
     6781 ctccacgcag aactggtcga gtgactgacc tcgtccagct gcttgcgggc ctcgtccttc
     6841 ttgcgctcca ggtcctgcag ggctttctgc atgtccttcc tggcttccac ggaggcggcg
     6901 ctggttttct tctggagttc agtgatcttc gtctgcatct ccgccagttc ggcttgaacc
     6961 gtcttttgaa agctgtcctt ttgctggatc gtatactgct tcgtggactc gacggcctcc
     7021 ttcgtgtccc ggatcatttt ttcggtggtc gacggcgggg cctggtctcc ggcagcgatg
     7081 gccaaggggg cgagaccaca cccgacagcc gtgagacaga ggaccacgag cactctttct
     7141 gcaatcatgg ctgggactcc ttcatgaatg gaaacagcct gtaagacaag gggtgaagcc
     7201 gatgcggaat acctcaaatt atctggcatg ttctgtgccc ggagccttct ccgcgggagg
     7261 ttaaggtttt cggcgaaatg agccgaaaag gatctacaac gaagacaatc ccgcaacctt
     7321 gacctgtggt gtgagcccta tgagtgctga acttgcccca atgtcagatc cggcctcaac
     7381 cagcccgtta gaacagctct tgatcgaacg gatccgcaaa ggagccatcg aactcccgct
     7441 gttgccgcag gttgcctccc gcattctggc catggtgtac gatcccgatg ccgaggcggc
     7501 aaaactggcc gcgctcattc accaagacca ggcgctggcc gcccatgtca tccggattgc
     7561 caattccccg gcgtatatga cccgtaatcc ggtggtttcc ctccagcatg
//
LOCUS       DAOQDS010000110         7515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_5585_length_7515_cov_24.010322, whole genome shotgun sequence.
ACCESSION   DAOQDS010000110 DAOQDS010000000
VERSION     DAOQDS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7515
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5585_length_7515_cov_24.010322"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..2170)
                     /gene="fusA"
                     /locus_tag="PK589_17550"
     CDS             complement(89..2170)
                     /gene="fusA"
                     /locus_tag="PK589_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNJ21471.1"
                     /translation="MARQTPLERTRNIGIMAHIDAGKTTTTERILFYTGMTHKMGEVH
                     EGAATMDWMEQERERGITITAAATTCFWRDHRINIIDTPGHVDFTIEVERSLRVLDGA
                     VAAFDSVQGVEPQSETVWRQADKYEVPRIAFMNKMDRIGADFYASVQSIIDRLGANPV
                     PIQIPIGREAEFRGSVDLISMKGYFYDDETLGAKYKIGEIPADMVDQANDYRQKMLDA
                     VVEFDDQVMEKYLNGQSLAEDEVRRVVRAGTIAMKIVPVICGSAFKNKGVQQLLDAVV
                     DFLPSPVDIPPVIGVDPATGKDVERSPSDTEPFSALAFKIMTDPFAGQLTFFRVYSGT
                     LKTGTPVLNVTKGTKDRVGRLLKMHANKREEIEIVYAGDIAAAVGLKSATTGDTLADE
                     KQPVLLEIMKFPEPVIAMAIEPKTKPDQEKMGFALQKLAQEDPSFRVRTDEETAQTII
                     AGMGELHLEIIVDRLMREFKVEANVGKPEVAFRETIRRKAEAESKYIKQTGGRGQYGH
                     VVMTVEPSEPGKGLEFVNKTVGGSIPKEYIPAIEKGVKERMETGVVAGFPLRDVKVTV
                     IDGSYHDVDSNEMAFKIAASMGFADACKKADPVLLEPIMKVEVLVPQDFMGDVIGNLN
                     GRRGKVQGMKVRAGAQAIEATVPLMEMFGYATDLRSRTQGRATYSMEFDRYDQVPRQI
                     AEAITAKHRGE"
     gene            complement(2284..2757)
                     /gene="rpsG"
                     /locus_tag="PK589_17555"
     CDS             complement(2284..2757)
                     /gene="rpsG"
                     /locus_tag="PK589_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002006497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNJ21472.1"
                     /translation="MPRGQFFGHREAQPDSKYRDKLVGKFLNVLMGGGKKSTAERICY
                     GAFDLIQEKTNGGDPMKIFKSAIDNVKPVVEVKSRRVGGASYQVPVEIRPSRRVSLAL
                     RWITEYSRSRGGKSMQDRLAAELLDASNNTGASVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2820..3191)
                     /gene="rpsL"
                     /locus_tag="PK589_17560"
     CDS             complement(2820..3191)
                     /gene="rpsL"
                     /locus_tag="PK589_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNJ21473.1"
                     /translation="MPTINQLVRKGRTLVKSKTKSPALKRCPQKRGVCLRVYTTTPKK
                     PNSALRKVARVRLTNGMEVTTYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGSL
                     DAVGVADRKQARSKYGAKRPK"
     gene            complement(3299..7429)
                     /gene="rpoC"
                     /locus_tag="PK589_17565"
     CDS             complement(3299..7429)
                     /gene="rpoC"
                     /locus_tag="PK589_17565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNJ21474.1"
                     /translation="MRIRIASPEKIRSWSYGEVKKPETINYRSFKPEKDGLFCAKIFG
                     PIKDWECNCGKYKRMKHRGIVCDKCGVEVIQSKVRRERMGHIELAAPVAHIWFLKGVP
                     SRIGTLLDMSLKQLEKILYFESYVMVDPGSTSMSEQELVSEEQLRSLQSEYGSGAFKV
                     GIGAEAIRELLRKVDINTQWDELHVKAKASASAALKKKYAKRLKVLEAFRRSGNKPEW
                     MIMDVIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLIELKAPGVIIRN
                     EMRMLQEAVDALFDNGRRGRAIRGPNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGR
                     TVIVVGPELRLHQCGLPKKMALELFKPFIFHKLEERGAATTIKSAKRLVEKERPEVWD
                     VLDEVIREHPVLLNRAPTLHRLGIQAFDPVLVEGKAIRLHPLVCAAFNADFDGDQMAV
                     HVPLSVEAQVEARVLMMSINNILSPANGKPIAVPSQDMVLGCYWLTKERVGAKGEGKL
                     FGSPEEARIAYDAGALDEHARIKVRCNGTLVQTTAGRVILSEILPPMMPFADANKLMT
                     KKEMSKLIDAVYRQAGHRETVTFLDKIKDLGFHYATRAGMSICIDNMHIPSRKEDLIG
                     KAQHEVNEIEKQYSEGLITNGERYNKVIDIWAHVTEQVANEMMKELGAGGDPAKAESF
                     NPIFMMADSGARGSSQQIRQLGGMRGLMAKPSGEIIETPITANFREGLTVLQYFISTH
                     GARKGLADTALKTANSGYLTRRLVDIAQDVIVTEEDCGTTDGILVSALLEGGEIIQPL
                     EERLLGRLAGEDIRDPVTGEIIVSFNEEIDEEQAKAVVEAGVDRVKIRSVLTCQSARG
                     VCRLCYGRDLSRGRLVEKGEPVGVIAAQSIGEPGTQLTMRTFHIGGTASKVVEQTVLE
                     AKHAGHLKYMSLDAKKNADVHNAGIAVRNKEGEWVVMNRNAKIAIVDDSGREREKYPV
                     VYGAKIKLKDGDRIEVGQKLVEWDPYSLTILTETGGKVAYGDILEGVTMKEEFDEVTG
                     LSRKVIIEQSGATLRPRVSIKDDSGKTAKVSGAGAPVARYLLPVGAHIFVEKGAMVHP
                     GDVLAKIPRETTKTKDITGGLPRVAELFEARKPKEQAVISEIDGEVSYGGFVKGMRKV
                     LVDNKMGDVKEYFIPKGKHVNVHEGDWVRAGEPLMDGSANPHDILDVLGPKELQKYLV
                     DEVQDVYRLQGVSINDKHIEIIVRQMLRKVRIEDPGDTSFLPGSQVSKGMFDVENQRV
                     LEKDGKPALGKPVLLGITKAALTTDSFISAASFQETTRVLTEAAINGREDNLLGLKEN
                     VIVGRLIPAGSGFEEYRETFVASAKAPGELSGDQSQPVAVGQAPITSVGSASENNA"
BASE COUNT         1717 a         2335 c         1730 g         1733 t
ORIGIN      
        1 cgatcgtccc gatgttcacg tggggctttc gtcgctcaaa tttcgccttc gccatccccg
       61 atcctcccta gttcacacaa cccagaccct actctccacg atgcttagca gtaatcgctt
      121 ccgcgatctg ccgaggcacc tgatcgtagc ggtcaaactc catactgtat gttgcacggc
      181 cctgcgtacg ggaacgcaaa tccgtcgcat atccgaacat ttccataaga ggcacggtcg
      241 cttcgatcgc ttgagccccg gcccgaacct tcatgccctg aacttttccg cggcggccat
      301 tcaggtttcc gatgacatcg cccataaagt cctgaggcac caacacctcc accttcatga
      361 ttggctcaag aaggacagga tccgccttct tgcaagcatc ggcaaagccc atcgaggcgg
      421 caatcttaaa tgccatttcg ttcgagtcaa catcgtgata cgaaccatca atcacggtga
      481 ccttcacatc tcgcagcgga aatccggcaa caacaccggt ttccattcgc tccttcacac
      541 ccttttcgat cgccggaata tattctttcg ggatagagcc accaactgtc ttattgacga
      601 actccaatcc cttcccaggc tcggatggct caacagtcat caccacgtgg ccatactgcc
      661 ctcgaccacc ggtctgcttg atgtacttcg attcagcctc agccttccgg cgaatcgttt
      721 ctcggaatgc cacctcaggc tttccgacat tcgcctcaac cttaaattcg cgcatcaaac
      781 ggtcgacaat aatttcgaga tgcagctcac ccattccagc aataatggtc tgggcggtct
      841 cttcgtccgt gcgaacgcgg aaggatggat cttcctgggc taacttttgc agtgcaaagc
      901 ccattttttc ctgatcaggc ttagtcttag gctcgatagc catcgcaata accggctcag
      961 gaaacttcat gatttccaac aaaaccggtt gcttctcgtc cgccagcgtg tcaccggtcg
     1021 tcgcgctctt gagaccaacc gcagcagcga tatcgccggc atacacaatc tcgatctctt
     1081 cacgcttatt tgcgtgcatc ttcaagagac gaccaacccg atctttcgtc cccttcgtga
     1141 cattgagaac aggagtcccg gtctttaacg ttccggaata gacccggaag aaggtcagct
     1201 gtccagcaaa agggtccgtc ataattttga atgcgagagc ggaaaatggc tcggtatcgg
     1261 aagggctacg ctccacatct ttcccggtgg caggatccac accaataact ggaggaatat
     1321 caaccgggga cggaaggaaa tccacaactg catccaataa ttgctgcaca cccttattct
     1381 tgaaagccga accacaaata acaggaacaa tcttcatcgc aatggtgccg gcacgaacaa
     1441 cgcggcgaac ctcatcctca gccaaggact ggccgttaag atacttctcc atcacctgat
     1501 cgtcgaattc gacaacggca tcgagcattt tctgccgata gtcattggcc tgatcgacca
     1561 tatcagcagg aatttcaccg atcttatatt tcgcaccaag cgtctcgtcg tcgtaaaaat
     1621 agcccttcat cgaaatcaga tccaccgaac cgcgaaactc ggcttcacgt ccgatcggaa
     1681 tctgaatagg aacgggattc gcccccagcc gatcgatgat ggactggaca ctcgcataaa
     1741 aatccgcacc aatccgatcc atcttattca tgaaggcgat acgggggacc tcatacttat
     1801 cagcctgacg ccagacagtt tcagactgcg gctcgacacc ctgaacagaa tcgaacgccg
     1861 caaccgcacc atcgagcacg cgcagagacc gctccacctc aatcgtgaaa tcgacgtggc
     1921 ccggcgtatc aataatattg atgcgatgat cacgccaaaa acaggttgta gccgccgcgg
     1981 tgatcgtaat accacgctcg cgctcctgct ccatccagtc catggtcgca gcaccctcat
     2041 gcacttcacc catcttatgg gtcatgcccg tataaaagag gatgcgctca gtggtcgtcg
     2101 tctttccggc atcaatgtgg gccatgatgc cgatattcct tgtacgctct aacggtgtct
     2161 gcctagccac aactccccca gaaaaaacga acgtgccgcc atcagagccg gagaccattc
     2221 actgcgacac tcacacctac gagcaaatat cgcagcccgg ctcaacagca gcacaactca
     2281 acactaccaa cgataatgag caaaggcctt attggcctct gccatacggt ggacatcctc
     2341 acgcttcttc actgatgcac ccgtattatt ggatgcatcc aacaattcag ccgcaagcct
     2401 atcctgcata ctctttccgc cacgggagcg ggaatattcg gtgatccatc gcaaagccaa
     2461 cgagacacgg cgagacggcc gaatctcaac cggaacctga taggaggcac caccaacccg
     2521 acgagacttc acctccacaa ccggcttcac gttatcgatc gccgatttga agatcttcat
     2581 cggatcgccg ccgttcgtct tctcctggat cagatcgaag gctccataac agatccgctc
     2641 tgccgtgctc ttcttcccac cacccatcag gacgttcaag aacttcccga caagcttgtc
     2701 tcgatacttc gaatcaggct gcgcttctcg atgaccgaaa aattgtccgc gtggcatcgt
     2761 tgtcctataa aataaagtca gtattcattg cacgtacgcg caatgagagg atgaactatc
     2821 tacttgggcc gcttcgcccc gtacttggac cgggcctgct ttctatcagc aactcccacc
     2881 gcatccaaag aaccacgcac aatgtgataa cgaacgcccg gcaagtcctt cacacgacca
     2941 ccacgaacca ggacgatcga gtgctcctga agattatgac ccacaccagg gatgtaggtt
     3001 gtcacttcca tcccattggt caaacgcaca cgcgcaacct tacgaagcgc ggagttaggc
     3061 ttcttcggag ttgtggtgta gacacggaga cagacgccac gcttctgcgg gcaacgcttc
     3121 aacgcgggac ttttcgtctt cgacttaacg agcgtccggc cttttcgcac caactgatta
     3181 atcgtaggca tgcactatac tctttcttga aaatcaggca tttgctcaaa aatgctcctg
     3241 agcgaagccg tcggattata gagatgtgta ctacttatgt caagaacctt cttgttcttc
     3301 acgcattatt ttctgacgca ctgcctacag aagtgatcgg agcctgtcca accgccaccg
     3361 gttgagactg gtcaccggac aactctcctg gagccttagc gctcgcaacg aaggtctctc
     3421 ggtattcctc gaaaccgctg cccgccggaa tcaatcgtcc aacgatgacg ttttccttca
     3481 ggccaaggag attatcctcc ctgccgttta tggccgcctc cgtcaacacc cgggtcgttt
     3541 cctggaaaga tgccgccgag atgaagctgt cggtagtcaa cgcggccttg gtaataccca
     3601 gcaggaccgg ctttccgagg gctggcttgc cgtctttttc caagacacgc tgattttcca
     3661 cgtcgaacat gcccttactt acctgactac caggcaagaa ggacgtgtct cctggatcct
     3721 caatgcgaac ctttcgcaac atctgacgca caatgatttc aatgtgcttg tcattgatgg
     3781 acacgccctg aagccgatac acgtcctgca cttcatcaac aagatatttc tggagctcct
     3841 tcggccccaa cacatccaaa atatcgtgcg gattggcgga tccgtccatg agaggctcac
     3901 cagctcgcac ccagtcgccc tcatgcacgt tgacgtgctt acctttggga atgaagtact
     3961 ccttcacatc ccccattttg ttatcgacca gcaccttccg catacccttc acaaacccgc
     4021 cataggaaac ttcgccgtcg atttcactaa tgacggcctg ctctttgggc ttgcgcgcct
     4081 cgaacaattc ggcgactcgc ggaagacctc cagtgatgtc cttggtcttt gtggtttccc
     4141 gcggaatctt cgccaacaca tcgccgggat gcaccatggc gcccttttca acgaaaatgt
     4201 gcgcgccgac cggcaacaga tatcgagcca ccggtgctcc agctccggag actttggcgg
     4261 tcttaccgct gtcatctttg atggaaacgc gcggccgaag cgtggccccg ctttgctcaa
     4321 tgatgacctt tcgagacaaa ccagtcacct cgtcaaactc ttccttcatg gtgacgcctt
     4381 cgagaatgtc gccatacgcg acctttccgc cggtctccgt gaggatggtc agtgagtacg
     4441 gatcccattc gaccaacttc tggccgactt cgatccgatc accgtccttc aacttgatct
     4501 tggcgccata cacgaccgga tacttttcac gctcacggcc actgtcgtcg acaatcgcga
     4561 ttttcgcatt gcgattcatg acgacccatt caccctcttt attccgaacg gcaatgccgg
     4621 cgttgtgcac atcggcattt ttcttggcat ccaagctcat atacttcagg tgaccggcat
     4681 gcttggcttc caagactgtc tgctcaacaa ccttactagc cgtaccaccg atgtggaacg
     4741 tacgcatcgt caactgagtg ccaggctcgc cgatggattg ggcagcaata acgccgaccg
     4801 gctcgccctt ttccaccagc cgtcctcgcg acaagtcacg gccgtaacag agacggcaca
     4861 cgccacgggc cgactggcat gtcaagaccg atcggatctt tacgcgatcc acaccggctt
     4921 ccaccaccgc cttggcttgc tcttcgtcga tttcctcatt gaacgagaca atgatctcgc
     4981 ccgtcaccgg atctcgaatg tcttcgccgg ccagacgacc caacagacgc tcttcgagcg
     5041 gctgaataat ctcgccacct tcaagcagcg cgcttacgag aattccgtcg gtcgtgccgc
     5101 agtcttcttc ggtcacgatg acatcttgcg cgatgtcgac caatcgacgg gtcagataac
     5161 cggagttcgc ggttttcaac gccgtgtcag ccaaaccttt tcgcgcaccg tgcgtcgaaa
     5221 tgaagtactg caataccgtc aacccttcac ggaagttcgc cgtgatcgga gtttcaatga
     5281 tttcaccgga cggcttcgcc atcagtccgc gcataccgcc aagctgacga atttgctgcg
     5341 agctgcctcg agcaccggaa tccgccatca tgaagatcgg gttgaaggat tcagccttgg
     5401 cgggatcacc gccagcgccc aactccttca tcatctcatt ggccacctgc tcggtcacat
     5461 gcgcccaaat gtcgatgact ttgttgtagc gttcgccgtt ggtaatcaac ccttcggagt
     5521 actgcttttc gatttcattg acttcatgct gagccttgcc aataagatct tccttccggg
     5581 aaggaatgtg catgttgtcg atacagatcg acatgccggc cctggttgca taatggaaac
     5641 ccaggtcctt gatcttatcc aagaaggtga ccgtttcgcg gtggccagcc tgccgataga
     5701 ccgcgtcgat cagctttgac atttccttct ttgtcatcaa tttattcgca tcggcaaacg
     5761 gcatcattgg agggagaatt tccgacagaa tgacgcgccc ggctgtagtc tgcacgagcg
     5821 taccgttgca tcgcaccttg atcctagcat gctcatccaa cgcccccgca tcgtacgcaa
     5881 tgcgcgcttc ttcgggagat ccgaacaact tgccttcgcc ctttgcaccc acacgctcct
     5941 tggtcaacca gtaacaacca agcaccatgt cctgtgacgg aaccgcgatc ggtttcccgt
     6001 tcgctggcga cagaatatta ttgatcgaca tcatgagcac gcgggcctca acctgcgctt
     6061 caaccgacaa cggcacgtgg accgccatct ggtctccgtc gaagtcggcg ttaaacgccg
     6121 cgcagacgag tggatgcagc cggatggcct taccttccac caagaccgga tcaaaggcct
     6181 gaattccgag tctgtgcaac gtcggagcgc ggttgagcaa caccggatgc tcacggatga
     6241 cttcatccag cacatcccac acttcaggac gctctttctc taccagacgc tttgcactct
     6301 taatggtcgt agctgcgcca cgctcttcca gcttgtggaa aataaacggc ttgaacagct
     6361 ccaatgccat ctttttgggc agaccgcatt gatgcaaccg cagctccgga ccgacgacaa
     6421 taaccgttcg gccggaatag tcgacgcgct ttccgagcaa attctgccgg aaacgtccct
     6481 gcttgccctt cagcatgtca ctcaatgact tcaatgggcg cttgtttggc ccacggatgg
     6541 cacggccgcg acggccgtta tcgaacagag cgtccaccgc ctcctgtagc atgcgcattt
     6601 cattgcgaat aattacgccg ggagccttga gttcgatcaa gcgcttcaag cggttgttcc
     6661 gattgatgac gcgccggtac aggtcattga ggtctgacgt cgcaaaccgg ccaccatcca
     6721 acggaaccag cgggcgtaat tccggcggca ataccgggat gacatccatg atcatccatt
     6781 caggcttgtt gccggaacgg cgaaatgcct ccaagacctt caagcgcttg gcgtatttct
     6841 ttttgagcgc agcggatgcg gaggccttcg ccttcacatg caattcgtcc cactgggtat
     6901 tgatgtccac cttgcgaagc aattcccgaa tggcttcggc gccaatccca accttgaacg
     6961 caccgctgcc atattcggac tgcaatgaac gcaactgctc ttctgagacc agctcttgct
     7021 cactcatgct tgtcgatcca ggatcgacca tcacgtagct ctcgaagtag aggatctttt
     7081 cgagctgctt gaggctcata tcaaggagcg tcccgatgcg gctcggcaca cctttcaaaa
     7141 accagatatg tgcgaccggc gcagccaact caatgtggcc catccgttcg cggcgcacct
     7201 ttgactgaat gacttcgaca ccgcatttat cgcagacgat gccgcgatgc ttcatgcgtt
     7261 tgtatttacc gcagttgcat tcccaatctt tgatcgggcc gaaaatcttg gcgcagaaca
     7321 agccgtcctt ttcaggcttg aacgaccgat agttgatcgt ttccggcttc ttgacttcgc
     7381 catatgacca cgagcggatt ttttcgggcg acgcaatgcg aatgcgcatc gagtcgaacg
     7441 acaccgaatc ccgcggcttc tcgaataatg tgtatacacc ttccaaggtt gatccctcct
     7501 taacggtcgc ctgag
//
LOCUS       DAOQDS010000111         7151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_6024_length_7151_cov_19.404594, whole genome shotgun sequence.
ACCESSION   DAOQDS010000111 DAOQDS010000000
VERSION     DAOQDS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6024_length_7151_cov_19.404594"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(511..966)
                     /locus_tag="PK589_17570"
     CDS             complement(511..966)
                     /locus_tag="PK589_17570"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNJ21475.1"
                     /translation="MIITCSDTRTPDTDTSGQLIQKLLKENGHTIAAYYLVKDEPAQI
                     KARITEGVANDSVQAIIINGGTGISRRDSTFEAVDAMLEKRLDGFGEVFRFLTYQDIG
                     SPAIMSRATAGIINGRVLFSTPGSENAVRLAMEKLILPELGHLVKELTK"
     gene            complement(1173..1499)
                     /locus_tag="PK589_17575"
     CDS             complement(1173..1499)
                     /locus_tag="PK589_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNJ21476.1"
                     /translation="MPKPKHHILVCTNARPPGHPKPSCGGQGSAQLLMNFNMGLMQRG
                     IMPGEVLVTGSSCLGPCEQGPTVVVYPEGTWYSKVTEADVATILDEHIKGGKPAEKLN
                     PDSIWK"
     gene            complement(1543..1986)
                     /locus_tag="PK589_17580"
     CDS             complement(1543..1986)
                     /locus_tag="PK589_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNJ21477.1"
                     /translation="MKFFFYADNLNPSQLKRRAPEHKFLFTAYLPDHSVQFCRWSTQW
                     RCGLASVVPSPGEKVWGAVFEITDEDLKILDEFEDDVPQGAYRHLPVTVITEDGEKLL
                     VTTHAANPIGKFKPKEHYIDWVLKGIKQWKLPEECSEQWKAYKPA"
     gene            2190..2645
                     /locus_tag="PK589_17585"
     CDS             2190..2645
                     /locus_tag="PK589_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21478.1"
                     /translation="MSKVIHYAVLLLLIVGGLVYWWMKPPGINPITDPRAAEALALVQ
                     THRAAGYPTILQAFNERARILADRKLGLRLGEWQVIKQDEQRYEVRIYMREQGTTQWF
                     ERDFIWHVDLGTKRVNAASLPADGLMPEGPDSGRAGTPPAGAPPFPPTM"
     gene            complement(2635..2961)
                     /locus_tag="PK589_17590"
     CDS             complement(2635..2961)
                     /locus_tag="PK589_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNJ21479.1"
                     /translation="MDSYDGYVTVAQADDIPPGACRTVEVDGIFLALSNVDGTFRAVD
                     NTCPHAGGPLGEGCLDGELIECPWHGWRFNVRTGERPENPEIRVTCVEVRVLGTNVQV
                     KVPLTL"
     gene            3160..3705
                     /locus_tag="PK589_17595"
     CDS             3160..3705
                     /locus_tag="PK589_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNJ21480.1"
                     /translation="MEMTLLLNSTYEPLRVLHWQKAITLLWQGKVEVLEVYDRQIHGI
                     SISIKLPSVMRLLKLVKLKDSHRAVKFSRINIFTRDGYCCQYCKHKFRTEELTFDHVV
                     PIAKGGRKTWENIVTACWRCNNRKSGRTPEEANMRLIKKPVKPRWSPTVTITIGIRNT
                     PESWRDYLYWNLELDADSPDT"
     gene            complement(3718..4170)
                     /locus_tag="PK589_17600"
     CDS             complement(3718..4170)
                     /locus_tag="PK589_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21481.1"
                     /translation="MAGYTGRSLAMAGGIIGLAAVAAYFGVAHIGEPDQGGGSIAGQV
                     QIAPNLADQVKPTDVLFVIVRRPTGPPRPIAAKRFDGPKFPVQFEITNDDVMVKGNEL
                     KGMVDVIARLDRDGQAGQPQPGDMEGRYPKNPTLPGGRDLMITIDKVY"
     gene            4343..5002
                     /locus_tag="PK589_17605"
     CDS             4343..5002
                     /locus_tag="PK589_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNJ21482.1"
                     /translation="MAANSGFQISLDVRGWPVLVIGGNEEAAEKVQRLLDAGAKVTVV
                     SPTLHEMLRKLAASAKIIHRGRHFRANDLESVILVLNTLRDDRELAHSLIRLAREQKF
                     LLWSVDQPDVSNVVMPAVVSSGHVRVAISSSGQAPALSGFMKEDLERILDSEFAAFVD
                     WLAQLREQAKANEPDAEKRRALLREALDGFRLLGKVQYPKVWQEQRASQQAPAAPAST
                     T"
     gene            5019..5327
                     /locus_tag="PK589_17610"
     CDS             5019..5327
                     /locus_tag="PK589_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HNJ21483.1"
                     /translation="MVLLEFSMSPLGKGESVSKYVARSLDIIDKSGVDYRLNPMGTVL
                     EGEWDEVMAVVKKCYMRMRKDCNRVSCSIKIDYRKGPKGRLSGKVSSVEKQLKRKLKI
                     "
     gene            complement(5334..6755)
                     /locus_tag="PK589_17615"
     CDS             complement(5334..6755)
                     /locus_tag="PK589_17615"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNJ21484.1"
                     /translation="MICWIPLTQQHLMQWFALVLLAAPVTSAIAAGTQASAPDGTKPS
                     AMTIHIPIPVTPLAELYGNVNQQLAAHRSRGREWVTLGGGSGFLKYRLWPDEQGSTTA
                     GDRLLSQSTVPFGVEYAKQIKGSITKIAECGQRDASTGTGRLSVTVATMFKQGRSYTV
                     VPASQVSAVQSAQSCVLSEQGVDASPLMVQVYRSDLMEVLPAIDRKAASLVPIKPAVA
                     RIWNDLQEPMLLDETEQLWLMLNPDTTAAAGVAPLSGSPAAGYGVIARPTVVRGTKPA
                     SRHLPLPEPQDHFRDNGFHVDFDLQVPIEEANQRLREAVIGQEWSLGVGTIKIVNATL
                     YPLGHQVGVELVLRGLLPLTLRLKGTPAYDESAGRILFREVDYTIKERTPATDLAEEW
                     LHEPLREELAGRLSLPIREELDLMRQALEKGLNRELTGGRLRGTVRQLSLEDLAVQTA
                     SLSVRFKTEGTLRYDARPDVTAP"
     gene            complement(6903..>7151)
                     /locus_tag="PK589_17620"
     CDS             complement(6903..>7151)
                     /locus_tag="PK589_17620"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ21485.1"
                     /translation="RVPVIGRVCMDMTMVDVTDVQGVQVGQEAILIGQQGGERITAAD
                     LAAWQQTIPYEILCAIGPRVPRRYRPLMASTDSASRTS"
BASE COUNT         1418 a         2051 c         2115 g         1567 t
ORIGIN      
        1 ttgaaaatgg cctccagcag cgttctcggt cgcacggctc cctgcgacgt accgccaggg
       61 tacgcctcag tcgccgcgct tcctgcggcc ttgctggacg gccattttga acgtcctcag
      121 ccctcgttcg acatggcttc cagcagcgtt ctcgtctcat caaaatcctt gacgtaactt
      181 acgggtaggc ctccggtttt gcttcgcctg cggcctcgct gaatgaccgt tttgagtatc
      241 ctgggtcggg cagagggagc aagcggcttc cggcttcctt ctcgtttcat gaaattcctg
      301 caagcactcc tgcgggtacg cctcagtcgc cgcactcctt gtggcctcgc cggatgaccg
      361 ttttgagcat cctgcatgag gccgcgggaa aaccggcttc ctacttactt ctgggttcat
      421 cacactgagt gacatcaggt cacagcagtc agttctgagc aaacctcaaa gctgaagagt
      481 gattgctgag agctgatcgc tgacgacgtg ctatttcgtg agttctttga ccaggtgacc
      541 gagttcgggg aggatgagtt tttccatcgc caggcgcaca gcgttttccg agccgggagt
      601 ggagaagagc acgcggccgt tgatgatgcc ggcggtggcc cggctcatga tggccggtga
      661 gccgatgtcc tgataggtca aaaaccgaaa gacttcaccg aatccatcca gacgtttttc
      721 cagcatcgca tccacggctt caaacgtcga atctcgccgg gagattccgg ttccgccatt
      781 aatgatgatg gcttggaccg agtcgtttgc taccccttcg gtgatccgcg ccttgatctg
      841 cgccggctca tccttcacga gataataggc ggcgatcgtg tgcccgttct ccttcagaag
      901 cttctggatc aactggccac tcgtgtcggt gtcgggtgtg cgggtatcgc tgcaggtgat
      961 aatcatgcaa ccgacagagc ggggcgcgtg gtgtttgtgt tctttgtggc taggggtgct
     1021 catagcaatt gaatgacgaa catgccttcc agcgtcgttc tcagctcttc acgctcctca
     1081 acgtacccct gaggataccc tccaggtgtg agtcgcctgc ggccttgctg gaaaggcatg
     1141 ttgatcatcc tcaacgagga ggatgatccg agctacttcc agatgctatc gggattcaat
     1201 ttttctgccg gcttcccgcc cttgatgtgt tcgtcaagga tggtcgcgac atcggcttcc
     1261 gtgactttgg aataccaggt gccttcggga tagacgacta cggtcggtcc ctgctcgcaa
     1321 gggcccaggc aggaagaccc ggtgaccagc acttcgcccg gcatgatgcc gcgttgcatc
     1381 aggcccatgt tgaagttcat gagcagttgc gcggaaccct ggccgccgca ggacggtttg
     1441 gggtggcccg gcggtcgggc attggtgcag acaaggatat gatgttttgg ctttggcatc
     1501 gtgacctcaa tatatgggta agtgacgatc tgtggcgatc tatcaggctg gcttatacgc
     1561 tttccactgc tcgctgcatt cttcgggcaa cttccattgt ttgatgccct tgagcaccca
     1621 gtcgatataa tgttccttcg gtttgaactt gccgatggga ttggccgcat gggtcgtgac
     1681 caggagcttt tccccgtctt ccgtgatcac cgtgaccggc aggtgccggt acgcgccctg
     1741 cgggacatcg tcctcgaact cgtcgaggat cttgagatct tcatcggtga tttcaaagac
     1801 ggctccccag actttctctc ccggcgacgg gacgacgctg gccaggccgc agcgccactg
     1861 cgtcgaccag cggcagaatt gcacgctatg gtcaggcaga taggccgtga agagaaactt
     1921 atgttccggg gcacgacgct tgagttggga cgggttcaaa ttgtccgcgt aaaagaagaa
     1981 cttcattgaa cggtgaggct cccatggtgt cttgtaggtt gaccggtact tgtaccacag
     2041 cgctgcgcgg ccttgtcaag cgcggtaaga gtgcggcgcg tctcagtcga tggccgtccc
     2101 gcgagttcgt gaccgattga cagtattatc acctgccacc tatagtgatc agttcgttca
     2161 tcatgcatcg acggtgatcg ttacagctta tgagtaaagt cattcattac gcggtgctcc
     2221 tgcttctcat cgtcgggggg ctggtctatt ggtggatgaa gcctccgggc atcaacccca
     2281 tcaccgatcc gcgcgcggcc gaggcgttgg cgctggtgca gacgcatcgc gcggccggct
     2341 atccaacaat ccttcaggcg ttcaacgaac gagcgcgaat tttagcggat cgcaaattgg
     2401 gactgcgatt aggggaatgg caagtgatca agcaggatga gcagcgatac gaagttcgaa
     2461 tttacatgcg cgagcagggg acgacgcagt ggttcgagcg tgattttatc tggcacgtgg
     2521 atcttggcac gaagcgtgtg aatgcggcct ccctgccggc cgatggtttg atgcctgaag
     2581 gtccggatag cggacgcgca ggaactcctc ccgccggtgc gcctccgttc cctcctacaa
     2641 tgtaagcggc actttcacct gcacgttggt ccccagtaca cgcacctcta cacaggtgac
     2701 gcggatctcc ggattctccg gccgctcgcc ggttcgcaca ttgaatcgcc agccgtgcca
     2761 ggggcattcg atgagttcac catccaggca cccttcgccc agcggaccac ctgcgtgcgg
     2821 gcacgtattg tccacggcgc gaaacgttcc atcgacattg gagagcgcga ggaagatgcc
     2881 gtcgacctcc acggtacgac aggcgcctgg tggaatgtca tcggcctgag ccacggtgac
     2941 ataaccgtcg taagaatcca tgcgttcctt gtatgcgtta cagaatgatc cagattgatt
     3001 tacggccgag taacattctg caagctagag cctaagcgct tgatttcatt agtggcatat
     3061 ggcatagatg aagcgggaac cattcacgga agggtcaggc cagcgccgtc atttgctatg
     3121 ctgtaaatag tgccctcata ggagaccgcc ggagccacta tggaaatgac cttgttgctc
     3181 aactccacct atgaaccgct gcgtgtcctg cattggcaaa aggccatcac cttgctctgg
     3241 caggggaagg tcgaagtcct cgaagtctac gaccgtcaaa tccacggcat ctcgatctcg
     3301 atcaaactcc cctcggtcat gcggctcctg aagctggtca agctcaagga cagtcatcgc
     3361 gccgtgaagt tttcccgtat caacattttc acccgcgacg gctactgctg ccaatactgc
     3421 aaacacaagt tccgcacgga agaactcacg ttcgatcacg tcgtgcccat tgcgaaaggc
     3481 ggccggaaga cctgggaaaa catcgtgacg gcctgttggc gatgcaacaa ccggaagagc
     3541 gggaggacgc cggaagaagc caacatgcgt ctgatcaaga aacccgtcaa gcctcgctgg
     3601 agccccacgg tcaccatcac catcgggatt cgcaacaccc cggaaagctg gcgtgattat
     3661 ctctattgga atctcgagct ggacgccgat tctccggaca cctagcgtcc ttagccgtta
     3721 atagacctta tcgatcgtaa tcatgagatc gcggcccccg ggcagggtcg gattcttcgg
     3781 gtaccgtcct tccatatcgc ccggttgcgg ttggccggct tggccatcgc gatccaggcg
     3841 ggcgatcaca tcgaccatcc ctttcagctc atttcccttt accatcacat cgtcgttggt
     3901 aatttcgaac tggacgggaa acttcggtcc gtcgaatcgt ttggccgcga taggtcgcgg
     3961 aggaccggtt ggtcggcgca cgatgacaaa cagcacgtcc gtcggtttga cctgatcggc
     4021 cagattggga gcaatttgta cctgtcctgc gatcgatcct ccgccttgat ccggctcccc
     4081 gatgtgggcc acaccgaaat aggctgccac ggccgccagg ccgatgattc cgccggccat
     4141 cgctagggac cgtcctgtat atccagccac aatacactcc tttgttggcg caagagctct
     4201 gaaagcgacc gtcttgaact gcgggcgcat tatacccagc tcatagcgta aaggggaatg
     4261 gcgcggacgg tttaccctgc gaaatgccct gttgtatgat acgcgttcac gttattctcg
     4321 gcttgttgga ggaacatcaa tcatggctgc aaattccggg tttcaaattt ccctcgatgt
     4381 gcgcgggtgg ccggtcctcg tcattggcgg caatgaggaa gcggctgaga aggtgcagcg
     4441 gctgttggat gccggcgcca aggtgacggt cgtcagcccc acgctccacg aaatgcttcg
     4501 caagctcgcg gcctctgcga aaatcattca tcgcggacga cactttcgcg ccaacgatct
     4561 ggagagcgtc attctggtct tgaacacctt acgcgatgat cgcgaactcg cgcattcgtt
     4621 gatcaggctg gctcgcgaac agaaattcct gctatggtcc gtcgaccagc ccgatgtttc
     4681 gaacgtcgtc atgcctgctg tcgtcagctc cggccacgtg cgtgtggcga tcagctccag
     4741 cggccaggcg ccggccttat cgggattcat gaaagaagat ctggaacgga tactggatag
     4801 cgaatttgcc gcttttgtcg attggctcgc ccagctacgc gagcaggcaa aggccaacga
     4861 gccggatgcc gagaagcgcc gcgcactcct gcgtgaagcg ctggatggat tccgtctcct
     4921 gggcaaggtg caatacccga aggtctggca ggagcagcgc gccagtcagc aggcgcccgc
     4981 ggccccggca tcgacgacat aggaaggaca actggaccat ggtgttattg gaattcagca
     5041 tgtcgccgct gggcaagggc gaaagtgtca gtaagtatgt cgcgcgctcg ctggacatta
     5101 tcgacaagag cggagtggac tatcgattga atccgatggg cacggtgctg gaaggggagt
     5161 gggacgaggt gatggcggtc gtcaagaaat gctacatgcg catgcgcaag gactgtaatc
     5221 gcgtatcctg tagcatcaag atcgactatc gcaagggacc gaagggacgg ctctcaggca
     5281 aagtgtccag tgtcgaaaaa cagctgaagc ggaagctgaa gatctaatca cgctcatggc
     5341 gcggtgacgt cgggacgggc gtcatagcgc agcgtgcctt cggtcttgaa gcggaccgag
     5401 agactggcgg tctgcaccgc caggtcttcc agcgacaact gccgcaccgt cccgcgcaac
     5461 cgtccaccgg tcagctcgcg gttgagcccc ttttcgagtg cctgccgcat gaggtctaat
     5521 tcctcgcgaa tcggcagcga cagcctgccg gccaattctt cccgcagcgg ttcatggagc
     5581 cattcttcgg ccaggtccgt ggcgggggtg cgttccttga tggtgtaatc gacctcccgg
     5641 aacagaatac gccctgcgga ttcatcgtag gccggtgtcc ccttgaggcg cagggtcagc
     5701 ggtaataacc ctcgcaggac caactcgacg cccacctgat ggcccaatgg atagagcgtc
     5761 gcgttgacga tcttgatcgt ccccacgccc agcgaccatt cctgaccgat gacggcctca
     5821 cgcagccgct ggttcgcttc ttcgatcggc acttgcaggt caaaatcgac gtgaaacccg
     5881 ttgtcacgga agtgatcctg tggctcaggc aggggcagat ggcgcgacgc gggtttggtg
     5941 ccgcgtacga ccgtgggccg tgcgatcaca ccatacccgg ctgcgggact tcctgatagc
     6001 ggtgcgactc cggccgcggc ggtggtatca gggttcagca tgagccacaa ttgttctgtc
     6061 tcatccagca gcatgggctc ttgaagatcg ttccagattc gggcgactgc cggtttaatg
     6121 gggacgaggc tggccgcttt ccgatcgatt gcgggcagca cttccatcaa gtcgcttcga
     6181 tagacttgca ccatcaacgg gcttgcgtcc acgccttgtt cggacaagac acaggattga
     6241 gcagactgca ccgcgctgac ctgactcgca gggaccaccg tatagctacg accctgcttg
     6301 aacatcgttg ccaccgtgac cgaaagccga cccgttcccg tggaggcatc gcgttgtccg
     6361 cactcggcga ttttggtgat cgaccctttg atctgcttgg catattccac gccgaatggc
     6421 acggtgctct gtgacaatag tcggtctcct gcggtggtcg aaccctgttc gtctggccag
     6481 agccgatact tgagaaagcc gctcccacca cccaatgtga cccattcacg accgcggctt
     6541 cgatgggcgg ccagttgctg attgacgttc ccatacaact ctgcaagcgg tgtgaccgga
     6601 atcggaatgt ggatggtcat ggcggacggt tttgttccat cgggcgccga tgcctgggtt
     6661 cccgcggcta tcgctgatgt gaccggggcg gcaagcagga cgagggcgaa ccattgcatc
     6721 aggtgttgct gcgtcaacgg gatccagcag atcatgttca tgtcctcaga acacatgtga
     6781 gatggattgt gatcggactg cgcgtgtgcg gctacgtatc gactatgagg gaaccggggc
     6841 agggaaggca agcggatgtc gcgccgccgt tcggtaaaga cttgtgctga gtctcgaagg
     6901 tgtcagctgg ttcgggacgc gctatcggtc gaagccatga gtgggcgata gcggcgcggc
     6961 acgcggggac caatggcgca gagaatctcg tagggaatgg tctgttgcca ggcggcgagg
     7021 tcggctgcgg tgatccgttc accgccctgt tgtccgatca gaattgcctc ctgtccaact
     7081 tggacgcctt gcacgtccgt gacgtcgacc atcgtcatat ccatgcagac tcgtccgatc
     7141 accggcacgc g
//
LOCUS       DAOQDS010000112         7118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_6064_length_7118_cov_17.078437, whole genome shotgun sequence.
ACCESSION   DAOQDS010000112 DAOQDS010000000
VERSION     DAOQDS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6064_length_7118_cov_17.078437"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1112
                     /locus_tag="PK589_17625"
     CDS             111..1112
                     /locus_tag="PK589_17625"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNJ21486.1"
                     /translation="MKLVSFEVHTPVGIFRRIGAMRAGSIVDLNMAYARLLAEQRESR
                     PRRLADAQVPATMLEFLQGGASAMDAARRAFDFVAQDASLKGPSGETIIYNAADVHII
                     APLPNPASLRDFIAFEDHIAATSKRRGQPIPPEWYKAPVYYKGNHRTIVGPDYSLPWP
                     LNTQKLDYELELACIIGRDGIDVPERDAAQYIAGYTIMNDLSARDIQFQEMACRLGPA
                     KGKDFATAIGPCIVTPDEIPDLASLQMIARVNGEVWSQGRFGSIHWSFPQMIEHVSRG
                     EAIYPGDLFGSGTVGGGCGLELDRYLQPGDCIELEIQPIGILKTTIAGHNKRSREEA"
     gene            1109..1870
                     /locus_tag="PK589_17630"
     CDS             1109..1870
                     /locus_tag="PK589_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21487.1"
                     /translation="MMPVVTKSSEAAIVETVERQRAMYDGSDIDVRMQHLIRDTPHPT
                     LSSWDIQRLLRASRAVYFDSHVEVVSGHHTEVYLRFESIGRFPDLVTIIASNMAEWIL
                     QTFHHDPPAGILTTSSDARLLAKRTCEILATHLPLRTVLTPFNPDTGKIGTEIISGTV
                     APGERFLSLNDVTTRGMCVSKLGKVVTDHGGQLAGMMVFARRDSGQFPFMDELTATYP
                     FYFGVDLDMPQWEPADCHACRDGKPLVSWRDLPAL"
     gene            1907..2908
                     /locus_tag="PK589_17635"
     CDS             1907..2908
                     /locus_tag="PK589_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNJ21488.1"
                     /translation="MDQAGVAQDIKRSDLLEMYFYLRLTRSLEDRITALYRQGRIVGG
                     VYTSHGMEAIAVGYASALQSDDVIAPFHRDMGAFLIRGFSPGEIIAQYLGKQAGPTKG
                     KDGNVHMGDLKRGVIGFVSHLADNMPVAAGAALAFKIRGESRVAFAGTGDGGTSRGDF
                     HEAMNFAAVRKLPVVFFCTNNQYAYSTPVRYQMAITDVVERAKAYGMPGEIVDGNDVA
                     AVYLASRRAIAKARSGEGPTFLEFKTMRMHGHSEHDAATYVPRTLLDDWKTKDPILRA
                     EQLLMQLGYGDDSYFHEVGERAKKDVDAGTEFAEQSPLPEGRETLVGVFATQDEVLP"
     gene            2905..3903
                     /locus_tag="PK589_17640"
     CDS             2905..3903
                     /locus_tag="PK589_17640"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNJ21489.1"
                     /translation="MTTATATQETHTTYVDAISQALDEEMSRDERVFLMGEDIGAYGG
                     AFKVTEGFLKKYGEWRVLDTPLSESGFVGAAIGAAMMGLRPVVEMQFADFISCAFDQI
                     TEVAAKNHYRWGAAVPLVIRAPFGGGVHGGPFHSECPEGWFFHSPGLKIVAPSTPYDA
                     KGLLIAAIRDPNPVLYFEHKFLYRRIKATLPQEEYIIPLGKADVKRSGRDMSLITYGA
                     MVHLALEAAEVLQQEGIELEVVDLRTLIPLDKETLYASVRKTSKAIILHEDNKTGGIG
                     AEISALLTEDCFDCLDGPILRIAPPDTPVPFSTPLEEFFLPKVSDIVAGARKLAAY"
     gene            3945..>5052
                     /locus_tag="PK589_17645"
     CDS             3945..>5052
                     /locus_tag="PK589_17645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNJ21490.1"
                     /translation="MATDIVMPQLGESIAEGTVVKWLIQAGGAVEKDQPLLEVETEKV
                     ALDVPSPAAGFLAEVMVQEGTTVPVGTLLARLETQPAAAGVVNRVGDVGVRARQEQQP
                     GEQHYSPAVKQLAREHGVDLTVVPGTGEGGRVTKKDLLDYVSTRQQPSGEPPRTAQAP
                     TVVPQPRETPEEIIPFTQMRKTIADRMLLSRRTSAHVTTFFEADFRGIDIFRTGRTLT
                     YLPFVISAVTRAMKDLPLLNASWRDQGLAIKKAVHIGIAVALDEGLLVPVIRHADQKG
                     LTQLAHEVAELAERARNKRLTPDDVLDGTFTITNHGGFGSLFSTPIIHQPQTAILGVG
                     SVQKRAVVIDDAIAIRPMCYLSLSFDHRIIDGATA"
     assembly_gap    5053..5152
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5266..6471
                     /locus_tag="PK589_17650"
     CDS             5266..6471
                     /locus_tag="PK589_17650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNJ21491.1"
                     /translation="MTARHHAVIVSAVRTPMGSFNGGFSSIPSTKLGSIAIAEALQRI
                     QLTGEQIDEVLMGCVLSAGLGQAPARQAAIGAGLPHRVGAVTVNKVCGSSLQTVIMAA
                     RAIALGEAQIIIAGGMENMTRAPFLLEKARQGYRLGHGELTDSLIKDGLWDVYNQFHM
                     GNAGELCAARYQFSRQQVDDFAMESYSRAREAITAGSFTREIVPVEVPQKKGAPLMIA
                     DDEEPNRVDLSRLRQLKPVFQDDGVLTVGNSPSCNDGAAALVVMAEEEAHRLGLPPMA
                     RIVGYAGAALAPEWFTIAPIDAIRRLLKQTELTVSDIDLFEINEAFSAVSLAITRELG
                     LDAKKVNVNGGAVALGHPIGATGARILTTLLYAMEERDAHRGIASLCIGGGEALALLV
                     ERPRKIQHQ"
     gene            6497..>7118
                     /locus_tag="PK589_17655"
     CDS             6497..>7118
                     /locus_tag="PK589_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNJ21492.1"
                     /translation="MESRMTIDDITTVGVVGAGQMGRGITQVLATAGWKVLLVDVTEE
                     ALEDATKKIWQGVTKAVEKGALRGDQAGSAMAIIHPIRHMQRLREAQVVIEAIPEDPV
                     MKRALFAQLGQICEPSTLLASNTSSISIASLGMVSGRPDRVVGIHFMNPVPVMRLVEV
                     VRAIGTSEQTMRLALELVQRAGKTAVVCKDFPGFIVNRVLIPMINEA"
BASE COUNT         1488 a         2097 c         2127 g         1306 t
ORIGIN      
        1 gaacagacca atgaagttgc cgtgatgatc gaatcacgcc acgcctttca ggtcatgccg
       61 gaattggaag gtgttgaact gaaggattac gccatgagtt ggagtcagcc atgaaactgg
      121 tcagtttcga ggttcacacc ccggtcggaa tctttcgcag gatcggcgcc atgcgggcag
      181 gatcaattgt cgatctcaac atggcctatg cccgcctgct cgcggagcaa cgggaatcga
      241 ggccgcggcg tctcgccgat gcgcaagtcc cggccacgat gctggagttt ctgcaaggcg
      301 gcgcctctgc catggatgcg gcccggcgtg cgtttgattt cgtggcacag gatgcctccc
      361 tcaagggccc atccggtgaa acgatcatct acaacgcggc cgatgttcat atcatcgcgc
      421 ctctgcccaa ccccgcctcg ctccgcgatt tcatcgcctt cgaagaccac attgccgcca
      481 cgtcgaaacg gcgaggccaa ccgattcctc ccgaatggta caaggcgccc gtgtactaca
      541 agggtaacca ccggaccatc gtgggcccgg attactcgct cccctggccg ttaaacactc
      601 agaagctcga ttacgaattg gagctggcct gcatcattgg acgcgacggc atcgatgtgc
      661 cggaacgcga cgcggcgcaa tacattgccg gctacacgat aatgaacgac ttgagcgcga
      721 gagatatcca gttccaggaa atggcctgcc ggctcggtcc tgcgaaggga aaagacttcg
      781 ccaccgccat cggcccttgc atcgtgacac cggacgaaat cccggatctt gcttcgctgc
      841 agatgatcgc ccgtgtcaat ggagaagtct ggtcgcaggg ccgcttcggt tcgattcatt
      901 ggtcattccc tcagatgatc gagcatgtct cgcgggggga ggccatctat cccggcgacc
      961 ttttcggctc gggaacggtc ggcggcggct gcggccttga actcgaccgg tacttacagc
     1021 cgggagactg cattgaattg gaaatccaac ctatcggcat tctgaaaacg acgatcgcgg
     1081 gacacaacaa gcgctcgcgc gaggaggcat gatgccagtt gtcacgaaat cttcggaagc
     1141 cgcgatcgtg gagaccgttg aacgacagcg ggcgatgtat gatggatcgg acatcgacgt
     1201 gaggatgcag cacctcattc gcgacacccc gcatcccacc ttgtcgtcct gggatattca
     1261 acgcctgctg cgtgcctcgc gcgcggtgta tttcgacagc catgtggagg tcgtgtccgg
     1321 tcaccatacc gaggtctacc tgcgattcga atccatcggc cgttttcccg acctcgtgac
     1381 gatcatcgcg tccaacatgg ctgagtggat tcttcaaaca tttcaccatg acccgccagc
     1441 cggaattctt accacgtcgt ctgacgcgcg gctgttggcg aaacgaacct gcgagatcct
     1501 ggcgacgcat cttcccttac gcaccgtgct gacgcctttt aaccccgaca cgggcaagat
     1561 cggcacggag atcatcagcg gaacggtggc ccccggggag cggttccttt cactcaacga
     1621 tgtcaccaca cgcggcatgt gcgtcagcaa gttgggaaag gtcgtcaccg atcacggcgg
     1681 gcagcttgcg ggcatgatgg tgtttgcgag gcgggacagc ggacagtttc cgttcatgga
     1741 tgagctgacg gccacctatc cgttttactt cggcgtggat ctggacatgc cgcagtggga
     1801 accggccgac tgccacgcct gccgggacgg gaaaccgttg gtgtcctgga gggacctgcc
     1861 cgcgctctga cgtgatgcgg atagccgatc gatgaaggag gcagctatgg accaggccgg
     1921 cgtcgcacag gacatcaaac gcagcgacct ccttgagatg tacttctacc tgcggctcac
     1981 cagaagcctg gaggaccgga tcacggccct ctatcggcaa ggccggatcg tcggtggcgt
     2041 ctacaccagc cacggcatgg aagccatcgc cgtcggctat gcgtccgcct tacagtccga
     2101 cgatgtcatc gcccccttcc atcgcgatat gggagcgttc ctgattcgcg ggttttcccc
     2161 cggagaaatt atcgcccaat acctcggcaa acaggccggg cccacaaaag gcaaagacgg
     2221 caacgtgcac atgggcgatc tgaaacgcgg agtgatcggc ttcgtcagcc acctggccga
     2281 caacatgccg gtcgcggccg gcgcggcact ggcgttcaaa atccgtggtg aatcccgcgt
     2341 ggccttcgca ggaaccggcg acggtggcac gagccgtggc gacttccatg aagccatgaa
     2401 cttcgcagcg gtgcgcaaac tgccggtcgt cttcttctgc accaacaatc agtatgccta
     2461 ctccacgccg gtccgttacc aaatggcgat caccgatgtg gtggaacgag ccaaggcgta
     2521 tggtatgccg ggcgaaatcg ttgacggcaa cgacgtggcc gccgtctacc tggcgtcccg
     2581 tcgagccatt gcgaaggcgc ggtccggcga aggtccgaca tttctcgaat tcaaaaccat
     2641 gcgcatgcac ggccattccg aacatgacgc ggccacgtac gtcccgcgta cgctgctgga
     2701 tgattggaag accaaagacc cgattctgcg cgccgagcag ttgctcatgc agctgggcta
     2761 cggggatgac tcctactttc atgaggtcgg ggaacgcgcc aagaaagacg ttgatgcagg
     2821 caccgagttc gccgagcaaa gcccgctgcc ggaaggacgt gagacgcttg tcggcgtgtt
     2881 cgccacccaa gacgaggtgt tgccatgacg accgcgaccg ccacgcagga gacgcatacc
     2941 acctacgtcg atgccatatc acaggccctg gacgaagaaa tgagccgcga cgaacgggtg
     3001 tttctcatgg gtgaggacat cggcgcgtac ggcggcgcat tcaaagtcac ggaagggttt
     3061 ctcaagaaat atggcgaatg gcgagtcttg gatacacccc tctccgagtc cggcttcgta
     3121 ggcgccgcca tcggcgccgc catgatggga ttgcgccccg tggtcgaaat gcagttcgcc
     3181 gacttcattt cctgcgcctt cgatcagatc accgaagtgg ccgccaaaaa tcactaccgc
     3241 tggggagcgg cagtccctct agtgatccgg gcgccattcg gcgggggcgt ccatggaggt
     3301 ccgtttcatt ctgaatgtcc ggaaggctgg ttctttcatt cgcccggcct caaaatcgtt
     3361 gcgccctcga cgccctatga cgccaaaggt ctcttgatag ccgccatccg cgatccgaac
     3421 ccggtcctct acttcgaaca taagtttttg tatcgacgga tcaaggcgac gctgccgcag
     3481 gaagagtaca tcattcctct cggcaaggct gacgtgaaac ggtccggccg cgacatgtcg
     3541 ctgattactt atggagcgat ggtgcatttg gccttggagg cggcggaagt gctgcagcaa
     3601 gaaggaatcg agctggaagt ggtggatctt cgcacgctca ttcctctcga caaagagacg
     3661 ctctacgcct ccgttcgcaa aaccagcaag gcgatcattc tccatgaaga caacaagacc
     3721 ggcggcatcg gggccgaaat ttccgcgctg ctgaccgaag actgtttcga ctgtctggac
     3781 gggccgattc ttcgcatcgc cccgcctgac acgccggtgc cgttcagcac accattggaa
     3841 gaattctttt tgcccaaggt cagcgacatc gtcgcgggag ccaggaaact cgcggcctat
     3901 tgatgggatg gactaggtaa acaggcatgc aggagacgtg atccgtggct accgacatcg
     3961 tcatgccgca gctcggtgaa agcatcgctg aaggtaccgt cgtgaagtgg ctgatccaag
     4021 ccggcggtgc agtcgaaaaa gaccaacccc tcctggaggt ggaaaccgaa aaggtggcgc
     4081 tcgatgtgcc gtctccggcc gcggggttcc tcgcggaggt gatggttcaa gagggcacga
     4141 cggtgccggt gggcacctta ctcgccaggc tcgagactca accggctgca gcgggggtcg
     4201 tcaaccgggt cggagacgtg ggtgtgcgcg caagacagga gcaacagccg ggcgaacagc
     4261 actattcgcc ggctgtcaaa cagctggcac gagagcatgg ggtggacctg accgttgtcc
     4321 ccggcacagg ggaaggcggc cgggtgacca agaaagacct gctggattat gtctccacgc
     4381 gtcaacagcc aagtggcgag ccaccacgaa cggctcaggc tccaaccgtc gtcccgcagc
     4441 cccgcgaaac gccggaggaa atcattccgt tcacccaaat gcgaaagacc attgccgacc
     4501 gcatgctcct gagccggcgc acctccgccc atgtcaccac ctttttcgaa gcggacttcc
     4561 ggggcatcga catattccgc acgggacgca cactcaccta cctgcccttc gtcatcagcg
     4621 ccgtcactcg ggccatgaaa gacctgccgt tgctgaatgc ctcctggcgt gaccaaggcc
     4681 tcgcgatcaa aaaagctgtc cacatcggca ttgctgtcgc gctcgacgaa ggcctgctgg
     4741 tgccggtgat tcgccatgcc gatcagaaag gactcaccca gttggcgcac gaagtcgccg
     4801 agttggcgga acgggccagg aacaaacggc tgacgccgga tgacgtgctc gacggcacgt
     4861 ttacgatcac caatcacggc ggattcggca gcctgttcag tacgccgatc attcatcagc
     4921 cacagacggc gattctcggc gtgggatcgg tccaaaaacg cgccgtggtg atcgacgacg
     4981 ccatcgccat ccgccccatg tgttatctga gtctctcctt cgaccatcgc atcatcgacg
     5041 gggcgacggc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatgtgtta
     5161 tctgagtctc tccttcgacc atcgcatcat cgacggggcg acggcggatc gattcatggc
     5221 caaggtgaaa cagcatctcc aacagagcga ttgggagaaa cttctgtgac cgccagacac
     5281 catgccgtca tcgtcagcgc ggttcgaacg ccgatgggaa gcttcaacgg cgggttcagc
     5341 tcgatcccct caactaaatt gggaagtatt gccatcgccg aggcgctgca acgcattcag
     5401 ctgacgggcg aacagatcga cgaggtgctg atgggatgcg tgctcagcgc cgggctcgga
     5461 caggcacccg ccaggcaagc ggcgatcgga gcaggactgc cgcaccgggt cggggctgtg
     5521 accgtgaaca aggtctgcgg gtccagcttg cagacagtca tcatggcagc cagggccatt
     5581 gccttgggtg aagcgcaaat catcattgca ggcggaatgg aaaacatgac ccgggccccc
     5641 tttctgctgg agaaagccag acagggctat cggcttggac atggagagct gacggacagc
     5701 ctgatcaagg acgggctctg ggatgtgtac aaccaatttc acatgggcaa cgccggagaa
     5761 ctctgcgccg ccagatatca gttttcacgg cagcaggtcg acgactttgc catggaaagt
     5821 tacagccgcg cgcgtgaagc catcaccgcc ggttcattca ccagggagat tgttccggtc
     5881 gaggtgcccc agaaaaaagg cgcaccactc atgatcgcag acgatgagga accgaaccgg
     5941 gtcgatctct caaggctgcg acagttgaaa cctgtctttc aggacgacgg agtgctgacg
     6001 gtgggtaact ccccgtcctg caatgacggg gcggcagcat tagtcgtcat ggcggaggaa
     6061 gaggcccacc ggcttggatt gccccccatg gctcgcatcg tgggatatgc cggagctgcg
     6121 ctcgcgccgg aatggttcac cattgcgccc atcgacgcga ttcggcggct cctgaaacag
     6181 acggagttga cggtcagcga catcgacctt tttgaaatca acgaagcctt ttcggcggtc
     6241 tccttggcga tcacgcgtga actcggattg gatgccaaga aggtcaacgt caacggcggc
     6301 gcggtcgcgc tgggtcatcc catcggcgcc accggcgcac ggatcctgac cacgctgctc
     6361 tatgcgatgg aggaacggga tgcccatcgc ggaatcgcga gcctctgcat tggcggcggt
     6421 gaggcactcg cactgctcgt ggaacggccg agaaaaatac agcatcagtg atcacacgtg
     6481 cgcaagagca gcagccatgg aatcgcgaat gaccatcgat gacatcacca cagtcggcgt
     6541 cgtcggcgcg gggcaaatgg gacgcggcat cacccaggtg ttggcgacgg ctggctggaa
     6601 agtgttgctg gtggatgtga cggaagaggc gctcgaggac gcgaccaaaa agatctggca
     6661 gggtgtcacg aaggcggtgg aaaaaggcgc gctccgaggt gatcaggccg gatcggccat
     6721 ggccatcatc catccgatac ggcacatgca acgcctgcgt gaggcgcagg tggtgatcga
     6781 agcgataccg gaagatccgg tgatgaaacg ggccctcttc gcgcaattgg gacagatctg
     6841 cgagccctcg accctgttgg cgagcaatac ctcttcaatt tcgatcgcca gcctcggcat
     6901 ggtgtcaggg cggcccgacc gggtcgttgg catccatttc atgaaccctg tgccggtgat
     6961 gcgattggtg gaagtcgtcc gcgccattgg cacgtcggaa cagacgatgc gcctggcgct
     7021 tgaactcgtt caacgcgcag ggaagacagc cgtcgtctgc aaggattttc ccggcttcat
     7081 cgtcaatcgg gtgctcattc ccatgatcaa cgaagcca
//
LOCUS       DAOQDS010000113         6922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_6334_length_6922_cov_21.539828, whole genome shotgun sequence.
ACCESSION   DAOQDS010000113 DAOQDS010000000
VERSION     DAOQDS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6922
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6334_length_6922_cov_21.539828"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PK589_17660"
     CDS             complement(<1..292)
                     /locus_tag="PK589_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNJ21493.1"
                     /translation="MKILLLLLGGLKFGKIALTGGTMLLSMVAYSFFFGWWYAVGFVL
                     LILFHELGHYAAAKQRNLNVGAPTFIPFVGAWIQLKEQPMDVETEAYVGIAGP"
     gene            complement(289..1188)
                     /locus_tag="PK589_17665"
     CDS             complement(289..1188)
                     /locus_tag="PK589_17665"
                     /inference="COORDINATES: protein motif:HMM:NF021454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HNJ21494.1"
                     /translation="MTWCHWRLMTAFLCWMVAGVALPALAEEPKPSGLHWLEGPTEAK
                     LGDQALLKLPKGYQFLGAQETQALLKRMGNFPSGSELGLITATGEGEQWFMVVRYIDA
                     GYVKDDDAANWDADALMTSIKEGTEEDNKNRQAQGFPPLIIRGWEEKPHYDKAANKVV
                     WAISAQEPESPVGVNYNTLALGRQGYLSMNMVGSLEQLPVLKPHVSQLLANVEFVEGK
                     RYTDFNSTTDKVAAVGLTALIAGAAVKSGLLAKLWAFIIPLLVAGKKLFMLLVIALGG
                     LAAKYLKKKPQAEQPGGGGGVSS"
     gene            complement(1368..1769)
                     /locus_tag="PK589_17670"
     CDS             complement(1368..1769)
                     /locus_tag="PK589_17670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNJ21495.1"
                     /translation="MKRSKALVLLDQREGEGPEAQRGDRVLYNCRLFLHCGDEVPLNE
                     RQAATLPGHMLRVDGGVTLVDHQVELGRRQVIPGIEQALLGMRVGGYRKVRISPHLAY
                     RDKGLPSLIPANALLVAEVWVRKIVKPEARE"
     gene            complement(1775..2887)
                     /locus_tag="PK589_17675"
     CDS             complement(1775..2887)
                     /locus_tag="PK589_17675"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HNJ21496.1"
                     /translation="MVSLQRFQSVLGKPFLPPLFFFSGVTYDTLVLTRIDRLRDNLLL
                     LAYLTLLGFLIVLTGRLGTQEATVDDLPPDAPFFMKWIVQAKPYAPMAIQFLLGGLFS
                     AYAIFYSKSATFAGTAVFFCVLVAFLVANEFLRSRLSNIQLLVSLYALVCFAFFTFFL
                     PVMTGWMNAVIFLAGAMVTVLIVLRVVQLIYWKNADRTTREAMWAGAPAVGLIALLVG
                     FYFMNWIPPVPLSLKFGGMYHEVKRSSDRYELSFEKSWYEVWKTSDNVIPADDPVYCF
                     TAVFAPVALKTTIYHHWYYRPDAGQPFADADRIPLKISGGREGGYRAYTFKEGLDPGD
                     WRVDVEAEDGRVIGRVAVKVLNKTTVGPLSLTTVVY"
     gene            complement(3151..4212)
                     /locus_tag="PK589_17680"
     CDS             complement(3151..4212)
                     /locus_tag="PK589_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21497.1"
                     /translation="MRERRWETAGRLTFQEILAVGERLGALGLKPAHPAKDVICYVED
                     WAVDSPEAFDQLDHWATEDVTLLHIREAWQGDFFLLAGGYHTVYQRFQDVGTYCSISH
                     PWRTKPGLRFHHPTSMFWLGFRHNHAFIRVRLHTQEVVTPGETREDARRAEWVDERRA
                     IFLDAIAMLDLPVETSVDKGSLVLTPADPATPFFCSWPDAFGPCQFEYNSLDAYEFLV
                     PASRLAATMASQPAGVRAYLTGFSDQALDAFAAVQPGAKTVYRCSVHCALGELPEVLE
                     TIAPQGRLYSTLCEFQTQELMPEGEDASAIIGIVGTAGGFQLEARLNRAPLPEEHMAG
                     WLERLLGMTVVYAPLPPFM"
     gene            complement(4258..4908)
                     /locus_tag="PK589_17685"
     CDS             complement(4258..4908)
                     /locus_tag="PK589_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21498.1"
                     /translation="MIAQGCRIAGQVCIAVGLALLWGCAGTGEMVSLNVQPQQPFVQS
                     GQPEPLKIVIEPFEDRRADKTKIGQRTHLGGGVTNFNVSGGSPGVTIAEALAEALRWR
                     GWNRRGWDARVVQSGVGVSGADIVIGGEIVDFSANAKSRVFNTKLTGESRLVIRAKNM
                     SDESSTQRNLQGEQSKLVFWFTSEDVENLMSGLLQDGIERFIADTKIEGRALKSAK"
     gene            complement(5042..5671)
                     /locus_tag="PK589_17690"
     CDS             complement(5042..5671)
                     /locus_tag="PK589_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21499.1"
                     /translation="MASPGADSLLTSDELRLVGDRDLFRSKERTILKVQALLTTLQAG
                     LQADLAHMTLLFPPEFDPAKAQSVKGERLEQCPYQYLDYPKHFHGDDKFTFRSLCWWG
                     HHLVFAMIVEGGQAKQCKKNFFDRFHQLAGQGLELSLAPTLWEWKRGEGYTLPITHDR
                     KAQLAAVLSGRAWFKIARFVPLDSPALREGRLPEIGRETFRSLFPLLSP"
     gene            complement(5751..6131)
                     /locus_tag="PK589_17695"
     CDS             complement(5751..6131)
                     /locus_tag="PK589_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21500.1"
                     /translation="MAQRFQSGAFVQQCFAVHPLCLSLKRIDDDGRLVIACTSCRMMH
                     LMTPDQVVGRVAAVPLGGEVESPKAVPTARESLEDCLRAHPVSVTIRELDVIRDQVGL
                     RCGECRRIFDMQVSAFESLQKSQS"
     gene            complement(6255..6590)
                     /locus_tag="PK589_17700"
     CDS             complement(6255..6590)
                     /locus_tag="PK589_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21501.1"
                     /translation="MTLLLDPQQRSTRPAWHVFFSHTLRYVLAPLLFISSGFFTANFI
                     VSAHYTWPRTSRVFVLTLTLLILAYEFIYKDRTIQSNAPDRAFNAVLYSCMIPYALGV
                     LMMLALAKL"
     gene            complement(6587..>6922)
                     /locus_tag="PK589_17705"
     CDS             complement(6587..>6922)
                     /locus_tag="PK589_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21502.1"
                     /translation="LLVFSSTPSLYFRPMNILRNGGGDWEPMLLGIEPRNCKVCNQGL
                     YRDKTMLRGGWSRCAVCDEFVHYSCLASGKISFLKQRPRICKTCKTAAEQAKQAPSSP
                     SSQPAPVGS"
BASE COUNT         1550 a         2121 c         1927 g         1324 t
ORIGIN      
        1 ccggcccggc aattcccaca taggcctcgg tttccacatc catcggctgc tctttgagtt
       61 gaatccaggc accgacgaag ggaatgaagg tcggggctcc cacattcagg ttgcgctgtt
      121 tggcggccgc gtaatgtccc aactcgtgaa acagaatcag cagcacgaat ccaaccgcgt
      181 accaccagcc gaagaaaaaa ctatacgcca ccatcgacag cagcatggtt ccgccggtca
      241 gggcgatctt gccgaatttg aggccgccga ggaggagcag gagaatcttc acgatgacac
      301 acctccgcct ccgcctggct gctccgcctg cggtttcttc ttcaagtatt tggcggccaa
      361 cccgccgagc gcgatgacca acagcatgaa taacttcttt cccgccacca acaagggaat
      421 gatgaacgcc cacagcttcg ccaacaggcc ggacttgacg gctgcgcccg cgatcagcgc
      481 cgtcaggccg accgccgcca ccttgtccgt cgtgctgttg aagtcggtat atcgtttccc
      541 ttccacgaac tccacgttgg ccagcagttg gctgacatgc ggtttcagca ccgggagctg
      601 ctccagcgac ccgaccatgt tcatgctgag atacccctgt cgtcccaacg ccagcgtgtt
      661 gtaattgacc cctaccggcg attccggctc ctgcgccgag atcgcccaca ccaccttgtt
      721 cgccgccttg tcgtaatgcg gcttctcttc ccagccgcga atgattaacg gcggaaaccc
      781 ttgcgcctgg cggtttttgt tgtcttcctc cgtgccttct ttgatggacg tcatcagcgc
      841 gtcggcatcc caattggccg cgtcgtcgtc tttgacgtag cccgcgtcga tatatcgcac
      901 gaccatgaac cactgctccc cttcaccggt cgccgtgatc aaccccagtt cggagccgga
      961 agggaagttt cccattctct tcaacaacgc ttgggtctct tgcgcaccaa ggaattggta
     1021 gcctttggga agcttcagca acgcctgatc gcccaacttc gcttcggtgg gcccctcaag
     1081 ccaatgaagg ccagacggtt tcggctcctc ggctaacgcc ggaagcgcca cgccggcgac
     1141 catccaacac agaaaggccg tcatcagacg ccaatggcac catgtcatgg cttcgtctcc
     1201 ttcgaggatg gagcaacgga acgtgcttca ggctgtacca tcgcttagat ggcgtcctgc
     1261 tttcctggga ctgcgattca tacaaacatt catcatccta cctgaataca ccgtagaagt
     1321 ggctcccctt tggtcttagg atggcattgc gaatgatctt gcctacgcta ttctctcgct
     1381 tcgggcttca caatcttccg tacccacacc tccgcgacca gcaaggcgtt agcgggaatg
     1441 agacttggca gccccttgtc ccgataagcc aggtgcggac tgatgcgaac tttgcggtag
     1501 ccgcctactc tcattccaag caacgcctgc tcgattccag gaatgacctg ccgccgtccc
     1561 agttcgacct ggtgatcgac gagcgttacg ccgccatcga ctctcagcat gtggcccggc
     1621 agagtcgccg cctgccgttc attgagcggc acctcatcgc cgcaatgcag gaacagacga
     1681 cagttgtaca gaacacgatc cccccgttgc gcctccggac cttcaccttc acgctggtca
     1741 aggagtacga gggccttcga acgtttcatc gagactagta cacaaccgtc gtcagagaca
     1801 gcggcccgac ggtcgtcttg ttcagcacct tgaccgcgac ccggccgatg acgcggccgt
     1861 cttccgcttc cacatcgacc cgccaatccc ccggatcgag tccctccttg aaggtgtagg
     1921 cgcgataccc tccctcgcgc cccccggaaa tcttgagcgg aatgcggtcg gcgtcggcga
     1981 agggctgacc ggcatcaggc cggtaatacc aatgatgata aatcgtcgtt ttaagggcga
     2041 ccggggcaaa caccgccgtg aaacaataga ccgggtcgtc cgccgggatg acgttgtccg
     2101 aggttttcca cacctcgtac caggatttct caaaactcag ctcataccgg tcgctcgagc
     2161 gcttcacttc gtgatacatg ccgccgaact tcagcgagag gggcacgggc ggaatccagt
     2221 tcataaaata gaagccgacc agcagtgcaa tcaagccaac ggccggcgca ccggcccaca
     2281 tcgcttcccg cgtcgtacga tcagcgttct tccaatagat caactgcaca acgcgcaaca
     2341 cgatcaggac tgtcaccatg gctccggcga gaaagatcac cgcattcatc cagccggtca
     2401 tgacgggcag gaaaaacgtg aagaaggcaa aacagacgag agcgtagaga ctgaccagca
     2461 actgtatgtt cgatagtcgg ctccgtagga attcgttcgc cacgagaaac gccaccagga
     2521 cgcagaaaaa gacggccgtc ccggcgaagg tcgcgctctt ggaataaaag atggcgtagg
     2581 cgctgaagag gccgcccagc aggaactgga tggccatcgg tgcatagggc ttggcctgca
     2641 cgatccattt catgaagaag ggcgcgtcgg gtggaagatc gtccaccgtg gcttcctggg
     2701 ttcccaaacg cccggtcagt acgatcagaa agcctaacag cgtcaggtag gccagcaaga
     2761 gcaagttgtc gcgaagacgg tcaatcctgg tgagcaccag ggtgtcgtag gtcacaccgc
     2821 taaagaaaaa cagcggaggg aggaatggct tccccagaac cgattggaac cgttgtaatg
     2881 agaccatgcg ctagatttca ggagacgcag gcgattgtca aacgaagaga ggaatcaggc
     2941 gatgaaattg gaaggcacga tcgagtcaca ataggcatgt cgcgggtcac gctacagcaa
     3001 aaggatctag cattcacggt ttagccggcc gctcaacacg ggcatccagc aaggccgcag
     3061 gggagaaaga accggaggcg tacccgctgc ggtacgtcga ggattctttc gacctgagaa
     3121 cgcagctggg gtccgtgttc agcggccgga ctacatgaag ggagggaggg gcgcatagac
     3181 taccgtcatg cccaatagtc gctccagcca accggccata tgctcctcgg gtagtggagc
     3241 gcgattgagc cgggcctcca gttgaaaccc tccggctgtg ccgactatcc cgatgatggc
     3301 cgacgcatct tctccctcgg gcatcaattc ctgcgtttgg aactcacaga gggtactgta
     3361 gagacggccc tgtggcgcga tggtctccaa cacttcgggc aattcaccca acgcgcaatg
     3421 taccgaacag cggtaaaccg tcttcgcgcc cggttgcacc gcggcaaagg catccaatgc
     3481 ctggtcggaa aatcctgtga gataggctcg cacaccggcc ggctgcgacg ccatggtcgc
     3541 agccaatcga ctcgccggca caagaaattc ataggcgtcg agtgagttgt attcgaactg
     3601 acaggggccg aaggcatccg gccaggaaca aaaaaacggc gtcgccgggt ctgcgggcgt
     3661 cagcaccagg ctgcccttgt cgacggatgt ctccaccggc agatccagca tggcgatggc
     3721 atcaagaaag atggcccgcc gctcatccac ccattcggcc cgccgtgcat cctcccgggt
     3781 ttctcccggc gtcaccactt cctgcgtatg cagacgcacc cggataaagg catggttgtg
     3841 acgaaacccc agccagaaca tggaggtggg atgatgaaac cgcaacccgg gtttcgtgcg
     3901 ccagggatga ctgatggagc agtaggtgcc gacatcctga aagcgctgat agacggtgtg
     3961 gtaaccgccc gccagcagaa aaaaatcacc ctgccaggct tcgcggatgt ggaggagggt
     4021 gacatcttcc gtggcccaat gatcgagctg atcgaaggcc tcgggagagt cgacggccca
     4081 gtcctccacg tagcagatca cgtctttggc gggatgagcc ggcttgaggc ccaaagcccc
     4141 tagacgctcg ccgaccgcca ggatctcctg aaaggtcagc cggccggcgg tctcccagcg
     4201 gcgttcacgc acaacgctcc tccgcggtcc gcgcgccgct gtcgaatctg gcgggcctca
     4261 cttggccgac ttcagcgcgc ggccctcaat cttcgtatcg gcgataaacc gctcaatgcc
     4321 gtcctgcaaa agaccagaca tgaggttctc gacatcttcg ctggtaaacc agaacaccag
     4381 cttgctttgt tcaccctgga gattgcgctg cgtgctgctc tcatcggaca tattcttggc
     4441 tcgaatcacc aggcgactct cgcccgtcaa tttcgtattg aacacgcggc tcttggcatt
     4501 ggccgaaaaa tcgacaattt ctccaccaat gacgatatct gcgccggaga cgcccacgcc
     4561 cgactgcacc acgcgcgcat cccatccacg acggttccag ccccgccacc gcaaggcctc
     4621 ggccaaggct tcggcgatcg tcacccctgg cgagcctccg ctgacattga aattcgtcac
     4681 cccgccaccc aaatgggtgc gctggccgat cttcgtcttg tccgcgcgac gatcctcaaa
     4741 cggttcgatg acgattttca gcggctccgg ctggccgctc tgcacaaacg gttgctgcgg
     4801 ttgcacatta agcgacacca tttctccggt tccggcacag ccccagagca gcgccaaccc
     4861 caccgcaata cagacctgac ccgcgattcg acatccctgt gcaatcacac atgcctcctg
     4921 tcagtatgtg gccttactaa aacgcgccca gtctacccaa ctcgcagccg gatgacaaac
     4981 gggaaagggc tggtttcagc aggaaaattc gccggtggtc cacgtcaaaa catgaagctg
     5041 gttaggggct gaggagaggg aagaggcttc gaaaggtctc ccgcccgatc tccgggaggc
     5101 gtccttcgcg taaggccggc gaatccaacg gcacaaaccg ggcgatcttg aaccaggccc
     5161 gcccggacaa tacggcggcc aattgagcct tccggtcgtg ggtgatcggc aacgtgtagc
     5221 cctcgccccg cttccattcc cataacgtgg gcgccagcga aagctccaag ccctggcccg
     5281 ccaactggtg aaaccggtca aagaagttct ttttgcactg cttcgcctga ccaccctcga
     5341 cgatcatcgc aaacaccaga tggtgccccc accagcataa cgagcggaag gtaaacttgt
     5401 catcgccgtg aaaatgtttc ggatagtcca gatattgata ggggcactgt tcgagacgtt
     5461 cccctttcac ggattgcgcc ttggctgggt caaactcggg aggaaacaac aatgtcatgt
     5521 gagccaggtc cgcctgtaac cccgcctgaa gcgttgtcag aagtgcttgt accttcagga
     5581 tcgtccgctc tttggaacgg aacaggtcgc gatcgccgac cagtctgagc tcatccgacg
     5641 tcaacaacga atcagcgccg gggctagcca tgggatccct ctctgtcatt gtttactgag
     5701 atcagtggcc ggagtgagac aggtgtgtgg tcacgggtga tgcgtgacgg tcacgattgg
     5761 gatttctgaa gagattcaaa cgctgagacc tgcatgtcaa atatgcgccg gcattcacca
     5821 cagcgcaatc ccacctggtc gcgaatcaca tccaattccc taatggtgac ggagacggga
     5881 tgggcacgca gacagtcttc cagtgattcc ctcgccgtcg gtacggcttt gggactctcc
     5941 acttcgccgc caagtggcac agccgcaact cgccccacga cctgatcagg cgtcataaga
     6001 tgcatcatgc gacaggacgt gcaggcgatc accaggcgtc cgtcgtcgtc aatccgcttg
     6061 agcgacaaac ataagggatg gacggcgaag cattgttgga caaaggcgcc gctttgaaac
     6121 cgctgtgcca tagccaccta ttgaaggatg agggacgccg gtgcctgctg gctcttcaga
     6181 acgtgatcga taggatgttc acgacacggt tcagcgaagc gagacaaccg ccgtgggtgg
     6241 ttttcaacat cctttcacag cttggccaac gccagcatca tcaacacacc gagagcatac
     6301 ggaatcatac aggaatacag caccgcattg aatgcccgat ccggggcatt cgactgaatg
     6361 gtgcgatctt tgtaaatgaa ctcgtaggca agaatcagca gggtgagggt caggacgaag
     6421 acccggctcg tgcgaggcca ggtgtaatga gcgctcacga tgaaatttgc cgtaaagaat
     6481 ccgctggaaa tgaagagcag tggcgcgagc acataccgca gcgtgtgcga aaagaacacg
     6541 tgccaggccg gcctggtcga acgctgctgc ggatctaata gcagggtcat gacccgacag
     6601 gcgcgggttg gctggacgga gacgaagggg cttgtttggc ttgctctgca gcagttttgc
     6661 aggttttgca aatacgcggg cgctgtttga ggaaactgat ttttccgctc gcgaggcagc
     6721 tatagtggac gaattcgtcg cagaccgcgc accgtgacca tcccccgcga agcatcgtct
     6781 tgtcgcggta caggccctgg ttacacacct tgcagttgcg gggttcaatc cccaacagca
     6841 tcggctccca gtcaccaccg ccattgcgaa gaatattcat ggggcggaag tataacgaag
     6901 gggtcgaaga aaaaacaagg ag
//
LOCUS       DAOQDS010000114         6907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_6361_length_6907_cov_14.105079, whole genome shotgun sequence.
ACCESSION   DAOQDS010000114 DAOQDS010000000
VERSION     DAOQDS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6361_length_6907_cov_14.105079"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            795..1142
                     /locus_tag="PK589_17710"
     CDS             795..1142
                     /locus_tag="PK589_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21503.1"
                     /translation="MSKPAAAKCYRFTSPMSVGIALTALIRAYGYHCHSPEPYSSHAC
                     AYLVENPHDAADTGGYTVTRKQESADEYLLTIHRTTASHLRTIIERARAETAAHSNIQ
                     IVSSRRAKPRAAA"
     gene            1283..2107
                     /locus_tag="PK589_17715"
     CDS             1283..2107
                     /locus_tag="PK589_17715"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21504.1"
                     /translation="MIPHDTPPPAQHASEKARRLADAFVGLLAQFGYTATLGAVVNYG
                     QKVSLSHPSLSHTLCAVIYVGKEKASFVKEGKNWPDGLYDSLLHKFRTLLEPELMVST
                     APIAQSAGSTVAYVDGSYCEEDRSAHIGWAFEIWRDGESIDGQAGSISHPDALSLRNV
                     AGECHAVEQVLEWCRANDCTEIEIRFDYIGLANWANGTWRTNAVQTQRYRERVESSGV
                     RITWTKVQAHTGEYGNTRVDYFARHAATHHAFFPELPVSILPAHFRRSRTAPVSCR"
     gene            2247..2645
                     /locus_tag="PK589_17720"
     CDS             2247..2645
                     /locus_tag="PK589_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21505.1"
                     /translation="MRMLALVMLILVLGVHSNLAKAEELTPNTLPTIEQPLTEMPFVE
                     GQGASYSVSDQTRACRQALPKAQLALAKSHAHMLRTLAVRPTSDPLIDITNRYDRHTR
                     ICTVTIRLTWPTVYTYPPVKLATDYRVPTP"
     gene            2706..3068
                     /locus_tag="PK589_17725"
     CDS             2706..3068
                     /locus_tag="PK589_17725"
                     /inference="COORDINATES: protein motif:HMM:NF022151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG family protein"
                     /protein_id="HNJ21506.1"
                     /translation="MKRVVLVSGSRDFPSKALLMDVMRQSFRAGDCLIHGCARGVDSW
                     AEEAARSAGVPVSRRPADWDRYGKRAGMLRNTAMLAETLAAPSYLILIFWDGQSRGTK
                     HMIDACQARGAAYSVVRP"
     gene            3148..3336
                     /locus_tag="PK589_17730"
     CDS             3148..3336
                     /locus_tag="PK589_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21507.1"
                     /translation="MGHTGFHLPASETLKLRTFRHVTDQQSVDALARLRARDREAEKN
                     NVGAIARAIRAGQSRITL"
     gene            3511..3867
                     /locus_tag="PK589_17735"
     CDS             3511..3867
                     /locus_tag="PK589_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21508.1"
                     /translation="MTRLDRGQTGKANGIDITVKSSRGPFRAFAPTWDMVMGHKRREL
                     SDEEYIARYRPILDRVPLAVWTALAARPACTFLCYCPPGVFCHSHVAIEYALARFPDW
                     FVDGRASASIPRRYTL"
     gene            complement(3913..4425)
                     /locus_tag="PK589_17740"
     CDS             complement(3913..4425)
                     /locus_tag="PK589_17740"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNJ21509.1"
                     /translation="MMGRTHCLVPAAIAAWLFGPDPTVLLISAGAGLFPDIDEPQSTI
                     GYRVFLLAKWFKGLVGHRTYSHSLVGVMTAASLALIFDMPWRWIEAGLTGWGSHLVLD
                     ALSGGVPLWWPWYATNRERWVLARWKPFGFADHLILVLAGVAFGAAAIHGNWLGWWIE
                     ILKRDITLSQ"
     gene            complement(4422..4835)
                     /locus_tag="PK589_17745"
     CDS             complement(4422..4835)
                     /locus_tag="PK589_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21510.1"
                     /translation="MPRQRWGALHEDYMNRFYELLHEARVRAPRLIGLWLNILLDEDT
                     PRIKRRFRGLDRYIEQMILKYPEYSARALNNLVRKQRQMGLNAEHVVRARIRMVKAKL
                     RSRAYRQAKKASLAGEVQWIGREEDARHLQNAGDA"
     gene            5381..6454
                     /locus_tag="PK589_17750"
     CDS             5381..6454
                     /locus_tag="PK589_17750"
                     /inference="COORDINATES: protein motif:HMM:NF037161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HNJ21511.1"
                     /translation="MSKANVEQPKASRGRQRTASSAPWVAIDVGYGYTKISTQEGQAH
                     VFPSLVAPAELSNIDLSLSDPQETVKLEGAEYIVGQAAVSRGFRFSEEYDGWWMTTRY
                     KALLHYAGANFVPPGSRIVTGIPLHIFGSQRAQQQISDVFRKALKAPAVAVLPQGFGA
                     LCLAISTNSALAQGRVALIDIGSRTTELICFSDGQYLNHESAGVVLGVGQVYTQVAQK
                     LSSQHQRQIDAYEVDWALREGKPIKIKGGVVERKALETLVQHYVRPLATRLHNEMTRL
                     WKEGAPGVDYLLYCGGGSALLAPYLGHFRDDYTILPDSQFTNAAGYLEYIRLTTQDQT
                     RAQDQGSAPVQQDDASAQKASHA"
     gene            6447..6737
                     /locus_tag="PK589_17755"
     CDS             6447..6737
                     /locus_tag="PK589_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21512.1"
                     /translation="MPRVTVYFSEKSAEDMRLFRWLVRLPPYQRGRNFKRLALARLIN
                     GAGRSRSKRVGEPAQTVPKAKIRDAHIPSTSTFMPVSLAETQAAFLRAIKKP"
BASE COUNT         1432 a         2113 c         1956 g         1406 t
ORIGIN      
        1 agtcgccgcg ttcgactaca aactggtccg cgagggcaag catcactaag cccgttcggt
       61 ggcctgccac cgggactcgg atcagccttc gctgacggag tcccggtcac ggccaggtgg
      121 gtagacgatc cccacccatc gcttgatcgt ccccgtggat tctgcggact aacagaatca
      181 ttcatcgacc tgggaggggt tcaggaaggt aggctttgaa gcagccaggc gggttggaat
      241 ccgcttacct ctaaggagta gtgataatcc cggtgcgacg gtcagcgctt tgcgcgagat
      301 acgctggcac acccctgaga tccgggggga gaaaacgcgc ccatgcctag ataatgctca
      361 cggacggaga actgaatgaa gccacgagta cgttcttcga gaaggcttgt acgctagggt
      421 ggcaagttgt agacggtgct tccgagaaat gaaaaataga cttttggcgt aatagcacac
      481 ctgaacgcct cccatctccc atacatgctc ctctcctcaa ttctggaggc actcatgcag
      541 acctttccgc tccttgcgat tgccatctgc gtgctccttc tggcaatcgc ggtcgggcgc
      601 gcctcccttc ggcgggtctt gcacgatcaa cgacgacggc actatcagcg ccacccactc
      661 tctctcgaac tacgacacct tgcgcctgcc tgccgtccta atcggcatcg atcctgacgc
      721 cacgcatcgg cgaccgggat ctcctgctgg ccctgcgggc agcgccggtg ttcgctgacc
      781 ttgatggagg gcgtatgtcc aaacccgctg cggccaaatg ctataggttc accagtccca
      841 tgagtgtggg tattgcgctc accgctctca tccgcgcgta cggctatcat tgtcattccc
      901 ctgagccgta ttcctcgcat gcgtgcgcct acctcgtcga gaatccacac gatgcagcgg
      961 atacgggcgg gtacaccgtc acccgcaaac aagagagtgc cgatgagtac ctgttgacca
     1021 tccaccgcac caccgcgtct catttacgga ccatcataga gcgggcgcgg gccgagaccg
     1081 cggcacactc caatattcag atcgtgtcat cgcgccgcgc aaaaccccgg gcggctgcct
     1141 aactacgcag ccgctaggtc gcacctcgac cgtacgccta ttgcgcaaat caccatacct
     1201 cagggctgcc gtgatattgg gccggacccg cgggccaact gcgcgggcag gggatcggca
     1261 ggtgtcacac aggaggtcca gtatgatccc acacgacact cctcctccgg cccaacacgc
     1321 aagcgagaag gcccgacggc ttgccgacgc gtttgtcggt ctgctggcac agttcggata
     1381 cacagcgacg cttggtgccg tggtcaacta cggacaaaaa gtctcgctct ctcatccctc
     1441 gttgtcccac accctgtgcg cggtcatcta tgtgggaaag gagaaagcct cgttcgtgaa
     1501 agaaggcaag aactggccgg atgggttgta cgactcactg cttcataagt ttcgtacgct
     1561 gcttgagcca gaactcatgg tttccacggc tcccatcgca cagtcggctg gatcgaccgt
     1621 cgcctatgtc gatggctcct attgcgagga agaccgcagc gcacacattg gctgggcctt
     1681 cgaaatttgg cgggatggcg agtccatcga cggccaggcc gggtccatct cccaccccga
     1741 tgcgttgagt cttagaaatg tcgccgggga atgtcacgcg gtcgaacagg tcctggaatg
     1801 gtgtcgggcc aatgactgta cggagatcga gattcggttt gactacatag gactggcgaa
     1861 ttgggcgaac ggcacctggc ggaccaatgc ggtccagaca caacgttatc gtgagcgagt
     1921 ggaatcttcc ggcgtccgaa tcacgtggac caaagtgcag gcgcataccg gagagtacgg
     1981 caatacacgc gtcgactatt tcgcgcgcca cgcagccacc catcacgcct ttttccctga
     2041 gctacccgtt tccatcctgc ccgcacactt tcgccgctca cggacagccc cggtttcttg
     2101 ccggtaatac agcggcagat ggctgagtga atctcttcgc caaatcggca gacaggtcca
     2161 gtcccggccc gctacgcggg caggccgcct cggcatgggc actcgtatga atcacgctgt
     2221 agagacacgt ctgtcgggag gctgttatgc gtatgctcgc tcttgtgatg ctcatcctcg
     2281 ttctcggggt tcattccaat cttgcgaagg cggaggagct cacccctaac accctcccga
     2341 ccatcgagca gccgcttaca gagatgccgt tcgttgaagg acaaggcgcg agttattctg
     2401 tgtcggacca gacgcgcgcc tgtcgtcagg cgttacccaa agcgcaactg gcccttgcca
     2461 aaagccacgc gcacatgctc cggacgctgg cggtacgtcc gacatccgat ccgctcatcg
     2521 acattacgaa ccgctatgat cgccacactc ggatctgtac ggtcaccatt cggctcacct
     2581 ggcccacggt ctacacctac cctccggtga agctggccac tgattaccgc gttccgacac
     2641 cctaacagct cgattcccaa ggctcatagc acgcttcgcg taggtgaact cttcgaggag
     2701 gtcctatgaa acgagtcgta ctcgtatcgg gcagtcgcga ctttccttcc aaagcattac
     2761 tcatggacgt gatgcggcag tcgttccgtg cgggggattg tctgatccat ggctgtgccc
     2821 gaggagtcga ctcctgggcg gaggaagccg cacgttcagc cggagtgccc gtgtcccgcc
     2881 gtccggcaga ctgggaccga tatggaaaac gggctggtat gctccgaaac accgccatgc
     2941 tggctgaaac cctcgcggcg ccgtcgtatc tgatcctgat cttttgggat ggccagtcgc
     3001 gcgggaccaa acacatgatc gatgcgtgtc aggccagagg cgccgcctac tctgtcgtcc
     3061 gtccatagct cgtgtgtggc tccctattgc ggctcgctac gctcgcaggg tcgaccccat
     3121 gtgactgttc accacaagga ggaatccatg ggacacaccg gctttcattt acccgcatct
     3181 gaaaccttga aactgcgtac gttccgtcac gtgacggatc agcagtctgt cgatgccttg
     3241 gctcgtctcc gtgcgcgtga ccgcgaggcg gagaagaaca acgtcggggc gattgcccgt
     3301 gcgatccgag ctggacagtc gcgcatcacg ctgtgacggc tcgccccacg ttcactccag
     3361 cgtagctgtg ctcttccaga agaagacaga cggcatacgg cggacaggac caatcggttc
     3421 tgtccgcgct cctctccacg tcgctcccaa accagcagag gccttcggca ggggaactcg
     3481 cttggtggcc actcaccaaa ggagccgagg atgacacgat tggatcgagg acagaccggg
     3541 aaagccaacg gcatcgatat tacggtgaag agcagccgtg gcccgtttcg ggcgttcgcg
     3601 ccgacctggg acatggtcat ggggcacaaa cggcgggaac tgtcggacga ggaatatatt
     3661 gcccgctacc ggccgatcct tgatcgtgtc ccgcttgcag tctggacggc gctcgccgcg
     3721 aggcctgcct gcaccttcct gtgttattgt cccccgggag tcttttgcca cagtcatgta
     3781 gcgattgagt atgcgctcgc gcgatttccg gactggttcg tcgacggcag ggcctcggcc
     3841 tctataccac ggcggtacac attgtagccg tagggccggc gcgagcatac gctcgcgccg
     3901 gcagaacctc tctcattgtg acaaggtgat gtcccgcttc aagatctcga tccaccaccc
     3961 gagccagtta ccgtgaatcg ccgctgcgcc aaacgctact cctgccagaa ctaagatcag
     4021 atgatcggcg aatccaaacg gcttccatcg cgccagcacc caccgctccc gattcgtggc
     4081 ataccatggc caccacaaag gtacaccccc gctcaatgca tctaagacca ggtggctccc
     4141 ccaaccggtc aaccccgctt caatccacct ccagggcata tcgaaaatca aagccaggga
     4201 ggcggccgtc atgactccga ccaacgaatg gctataggtg cgatggccca ccaacccctt
     4261 gaaccatttg gccaagagaa acactcgata gccaatggtc gattgcggct cgtcgatatc
     4321 cgggaaaagc ccagctcccg ctgagatcaa cagtacggtg gggtctggtc caaagagcca
     4381 ggctgctatt gcggctggta ccaggcaatg cgtacgcccc atcatgcgtc acctgcattc
     4441 tgcaggtgac gcgcatcttc ttcccgcccg atccattgca cctccccggc aagcgaggct
     4501 ttcttggcct ggcggtaagc ccgcgaccgt agtttcgctt tgaccatgcg aatccgagcc
     4561 cggaccacat gctctgcatt gagtcccatc tgacgctgct tcctcaccag attattcagt
     4621 gcccgcgccg agtactccgg atacttgagg atcatctgtt cgatataccg gtctaaccct
     4681 cgaaaccgtc gcttgatcct gggcgtatct tcatccaaca ggatattgag ccagagacca
     4741 atcaagcgtg gcgctcggac tctcgcctca tgcaacaact cgtagaaccg attcatgtag
     4801 tcttcgtgca atgctcccca tcgttgtcgt ggcatagaag cctcccgggg gaggtattac
     4861 cggatacagt aggccgtcag ggggcgagtg gggagacggt gacaagtctc aatataagac
     4921 accatatatg catccggtat acgaaatgcg tacgcgccgg tcctgtcgca ccctgctccc
     4981 gctgcgtacg ccggtgtcag ccggcacgat cgtgataggc atccgccgag ggagtcttgg
     5041 aagcgctctg gcagggcttc cggacagacc gggccaaggc tctgccccga cgcactcccc
     5101 agcaatccgc ctgataggtg tacgacattg tctgtgttag ggaggcctga ctcccccaca
     5161 cagccatgga tgtcgagccg atacagtgaa gtcgttgata acctgcggcg ttttagtttg
     5221 ggtgtgtgta cgcatgacaa cggaatgtct tacttatttg tctgacatgt atgacaaatc
     5281 gatttggtgg tatgcgagcc cgttcgaggt ccacgaagcc agtaatacat ccctagctga
     5341 gtgggaccaa tctgaaccgc atgacgaacg gaggctgtgt atgagcaagg cgaacgttga
     5401 acaaccgaag gcgtctcgtg gacggcaacg caccgcgtca tcggctccct gggtcgcgat
     5461 cgacgtcggg tatgggtaca caaagatctc gactcaagag ggccaggcgc atgtgtttcc
     5521 gtctctcgtg gcgccggcgg agctctccaa tatcgactta agcttgtccg atccacagga
     5581 gaccgtaaag cttgagggcg cggagtacat tgtgggccag gcggcagtgt cccgagggtt
     5641 tcgctttagc gaggaatatg acggctggtg gatgacgacg cgctacaaag cgctgctgca
     5701 ctatgccggc gccaacttcg ttccgccagg ctcccgcatc gtgaccggta ttcccctgca
     5761 tatcttcggc tctcagcgag cccagcagca aatcagcgac gtcttccgga aagcgctcaa
     5821 ggcgcctgcc gtggcagtcc tgcctcaggg attcggcgcg ctgtgcctcg cgatcagtac
     5881 aaactccgct cttgcgcaag gacgggtggc cttgatcgat atcggcagcc gcacgacgga
     5941 actcatttgt ttctccgatg gacagtacct gaatcacgaa tcggcggggg tcgtgttggg
     6001 ggtcggacag gtgtatacgc aagtcgccca gaagctctct tctcagcatc agcgacagat
     6061 cgatgcctac gaggtcgact gggcgttgcg cgagggaaaa ccgatcaaga ttaagggtgg
     6121 cgtggttgag cggaaagcct tagagacgct agtgcaacac tacgtgcgac cgttggccac
     6181 caggctccac aacgagatga cgcgactgtg gaaagaaggc gcgccaggcg tcgattattt
     6241 gctctactgt ggtggcggat cggccctcct tgctccctat cttggccact tccgagacga
     6301 ctatacgatt ttgccggaca gtcagttcac taatgcggcc ggctaccttg aatatatccg
     6361 ccttaccacc caggatcaga cacgtgcgca ggatcagggg tccgcgcccg ttcaacagga
     6421 cgacgcttcg gcacagaagg cgtcgcatgc ctagggtcac ggtctatttt tcagagaagt
     6481 cggcggagga tatgcgcctc tttcggtggc tggtccgact cccaccatat caacgcggac
     6541 gcaacttcaa gcggttggcg ttggctcgcc tcatcaatgg ggcgggccgt tcgcggtcga
     6601 aacgggtagg agagccggct cagacagtac cgaaggccaa gatccgtgac gcacacatcc
     6661 cttcgacctc cacattcatg ccggtgagcc tcgcggaaac tcaggccgcg ttcttgcggg
     6721 cgatcaaaaa accgtaacgt tgccgcccgt tgaggccgat tcttgattcc agggttgtaa
     6781 atcgaaggga atagatttcg gctatacttt cgtcaatttg atgattctgc ttgcgagctg
     6841 gacaagacta gaccaggacc ctcgccttaa aagggcccca ggcgataaat aaaatcaata
     6901 tcttaga
//
LOCUS       DAOQDS010000115         6862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_6426_length_6862_cov_21.412370, whole genome shotgun sequence.
ACCESSION   DAOQDS010000115 DAOQDS010000000
VERSION     DAOQDS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6426_length_6862_cov_21.412370"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PK589_17760"
     CDS             complement(<1..1025)
                     /locus_tag="PK589_17760"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ21513.1"
                     /translation="MNQPLNQIETALLAGDWDRLGYEAGVSVRALEAADEKDPRPYFA
                     LNVTHLIRGEFAEAWRVHAHALQETADIEKVGEWVKAVLKQYPDNPYLNLVQGMFLAQ
                     SGQSEQSVVFYKEAARLAPQSAYPHYFLAQIHERANHLEMAIKEYREAVKLDPAFAAA
                     RTNLGVAYQEQGRLEMAIPQYREVIKLNPNDGVAHANLGCALAEQGKFEPALQAYKEA
                     LRLNPNDAEIHFALGGVYETKGRMDLAVKEYRAATEANPELAPAHTALGWLLMEQSRP
                     SEAMEAFNRAVKANPEEAQALYGIGRIYAAKGKRESAAENFSKAIRFEKDPAKKNAIM
                     NALHASG"
     gene            complement(1046..2527)
                     /locus_tag="PK589_17765"
     CDS             complement(1046..2527)
                     /locus_tag="PK589_17765"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNJ21514.1"
                     /translation="MTTSARRVIALAPMPPEKSAYALARYSRSPDSIEDSIKWVHGHS
                     SEKFWEQFYFDYGHGSIADLGHVIICFEQISELAAIRLEDEPVWDGQAKSSRYQNFAT
                     SSWFVPATIRGQEIEASYLGILRGLATVYRALHDPLSQFLTEREPRPESMTPAAYQRA
                     ITARAFDVTRYLLPLAAQTNVGQVVSIRTLEKQITRLLSSQIPELRQIGEDLQEACRK
                     PPVNLWGELSGQSAGLGEPMAPTLARYAKPSVYQAEVYSDLARYAKDVLKGTGLDQPS
                     AYGAAEPVDLIEPHDPLDEVVTTLLYRASHAPYRKILALVQDWTEKQKQDTLEVAFQK
                     RGPYDELIKEFRSGYAFIFDVMMDIGGWRDMHRHRRCQQVQQNFTTVHGFETPPILAE
                     AGLEQEYREAMGHVKSDIERLKKSSQEAALYAIPFGFSVRCLFKMDYAEAEYIAKLRS
                     GVKGHWSYRTIAWLMKEKMTARYPILGARMQATPPDVQDALTR"
     gene            2743..3999
                     /gene="hisS"
                     /locus_tag="PK589_17770"
     CDS             2743..3999
                     /gene="hisS"
                     /locus_tag="PK589_17770"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNJ21515.1"
                     /translation="MIKAIKGVKDLLPEESPRWRFIEDTARRWALNYGFQEIRVPIFE
                     TTTLFARSIGATTDIVEKEMYTFADRDGSSLTLRPEGTAGTVRAFIEHNRAADPRPQK
                     YCYAGPMFRHERPQAGRLRQFHQFGVESFGVSDPRADVEVLSLLWRLLSDLTLPGLTL
                     EINNLGYADDRARYKPRLVAFLKGVESRLCGNCQRRIETNPLRVLDCKVPECRAATEE
                     APGLADSLSSPAREHFDHVKAGLTAIGIPFHLNPRLVRGLDYYCLTAFEVTCSHLGAQ
                     NAVGAGGRYDGLVRELGGPTVPAVGFALGLERIALMLPETFVVPAIPRVYVAAFGTQA
                     ADAGFVLLDELRRAGVPADMDFRSASLKAHLRQADRLGALYTILLGDDEVAKGTATVR
                     NMQTKAQEDLPIQDLATAFRNRLLNK"
     gene            4119..4439
                     /locus_tag="PK589_17775"
     CDS             4119..4439
                     /locus_tag="PK589_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNJ21516.1"
                     /translation="MGALKIGLLLSTPPSHPSVQTVASLASEAISQGADVYLYCIDEG
                     VKNLRDPRYTELVGRGMKLFVCAYGCQQHGVSTDQLDPRISLCGLVVLSNIVNGCDRF
                     LAFT"
     gene            4460..4810
                     /locus_tag="PK589_17780"
     CDS             4460..4810
                     /locus_tag="PK589_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21517.1"
                     /translation="MAATATIALIIRENPLTSAVPVEALRIALGLAAGENPITVILMG
                     PAVHLLAEESDDIVDLEILEKYLPSFEHLQIPFILVHSSGPRPDLQEGFAVTDGTVES
                     ARQAMAAADRVLVF"
     gene            4825..5097
                     /locus_tag="PK589_17785"
     CDS             4825..5097
                     /locus_tag="PK589_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21518.1"
                     /translation="MSEHKTLYLLRQPISDPAETLLPSAAETQLADRVSLVLLEAAAS
                     TTPSFPGPIYVLHQDSATPVQGGSRTQISYRELVTLIAEHPSTIVL"
     gene            5446..6789
                     /gene="dnaA"
                     /locus_tag="PK589_17790"
     CDS             5446..6789
                     /gene="dnaA"
                     /locus_tag="PK589_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNJ21519.1"
                     /translation="MNQMWNEALEYIQEKVPKQVYETWFTPVVLDRIEETTAYLAVPN
                     KFFGDWLGEHYHDLLAEAVSAAQGGGRMEVSFVVSQKAAPAQAQPDHSAADTAGRGFM
                     ASRSKRGIQLNPKYTFKSFVVGAGNQFAHAACMAVAEQPAKAYNPLFLYGGVGLGKTH
                     LLNAIGNYLAERSDLRIAYLTTEQFTNEVINSIRYDKMIDLRKRYRNVDMLMIDDIQF
                     LAGKERTQEEFFHTFNTLYEAHKQIVLSSDRFPKDMPDIEERLRSRFEWGLIADLQPP
                     DVETRIAILRKKSEDERIALPEDVIHFLANTMKNNIRELEGSLVRVGAYSSLTGQAIT
                     LEMAKNVLRDLIGDKKKIVSIEDIQEAVGSKYHVKIADLKSRRRSKTLVHPRQIAMYL
                     CRELTDASFPEIGRQFGGKDHTTIIHACRQITKAKEADSALHTTLEGLKEQILRA"
BASE COUNT         1410 a         1931 c         1992 g         1529 t
ORIGIN      
        1 tgaccgctgg cgtggagagc gttcatgatg gcatttttct tggcgggatc tttttcgaag
       61 cggatggctt tggagaagtt ttcggcagca ctttcccgtt tgcccttagc ggcgtagatt
      121 ctcccgatgc cgtagagcgc ttgcgcttcc tcggggttgg ccttcacggc gcgattgaag
      181 gcctccatgg cctcactcgg tcgggattgc tccatcaaca accagccaag cgcggtgtga
      241 gccggcgcga gttccggatt ggcttccgtg gccgcgcggt attccttgac cgcgagatcc
      301 atgcggcctt tcgtttcgta gactccgccc aatgcgaagt gaatctccgc gtcgttcgga
      361 ttgaggcgca gcgcctcctt gtaggcctgg agcgccggtt caaacttccc ctgctccgcc
      421 aaggcgcagc cgagattcgc atgggcgacg ccgtcgttcg gattgagctt gatcacttcg
      481 cgatattgag gaatggccat ttcgagccgg ccctgctcct ggtaggcgac gcccaggttg
      541 gtgcgggccg cggcgaatgc cggatccagt ttgaccgcct cgcggtactc cttgatggcc
      601 atttccagat ggttcgcccg ctcatggatc tgagccagaa aataatgggg ataggcggac
      661 tgtggcgcaa ggcgcgccgc ttctttatag aacaccaccg attgttccga ctggccggac
      721 tgcgccagaa acatgccttg taccaggttc aggtagggat tgtcgggata ctgcttcagg
      781 acggctttta cccattcgcc caccttctca atatccgctg tttcctgcaa tgcatgggca
      841 tgcacccgcc aggcctcggc aaattcaccg cgaatcaggt gtgtgacatt caacgcaaaa
      901 tatggccggg ggtctttttc gtcggccgct tcgagcgcac gaaccgagac cccggcttca
      961 taccctaacc ggtcccagtc gcccgcaagg agggccgttt cgatctggtt gagaggctga
     1021 ttcatgagac ggttcggtgc gccggtcagc gcgtcaatgc atcctgcaca tccggcgggg
     1081 tcgcctgcat gcgcgcgccg aggatgggat accgtgcggt cattttttct ttcatcagcc
     1141 aggcgatggt gcgataggac cagtgcccct tcacgccgga tcgcaacttc gcgatgtatt
     1201 ccgcctcggc atagtccatc ttgaacagac agcgcacgga aaatccgaat ggaatggcat
     1261 acagcgcggc ttcctggctg gatttcttca gccgctcaat gtccgatttc acatgtccca
     1321 tggcctctcg gtattcctgt tcgagcccgg cttctgcgag gatgggcggt gtttcgaacc
     1381 catgcaccgt cgtgaaattc tgctgcacct gttggcagcg gcggtgccga tgcatgtcgc
     1441 gccagccgcc gatatccatc atgacgtcga agataaaggc atacccgctg cggaattcct
     1501 tgatcaattc atcatacggc ccgcgtttct gaaacgcgac ttccaaggtg tcctgctttt
     1561 gtttctcggt ccagtcttgg acgagggcga gaatctttcg atacggagcg tgggaggcgc
     1621 gatagagcag cgtggtcacc acttcgtcca acgggtcgtg aggttcgatc aaatctactg
     1681 gttccgcggc cccataggcg gagggttgat ccaagccggt gcccttcagc acgtccttcg
     1741 cgtagcgagc caggtcactg tacacttcgg cctggtagac gctcggcttc gcgtaccgcg
     1801 ccaaggtcgg agccatcggc tcacctaatc cggccgactg acccgacaac tcaccccaca
     1861 gattgaccgg cggcttgcga caggcttcct gcaaatcttc tccgatttgg cgcaactccg
     1921 gtatctgcga ggacaagagg cgggtgatct gtttttcgag cgtcctgatg ctgacgacct
     1981 gccccacatt ggtctgcgcc gccaagggga gtaaataccg tgtgacatcg aaggcccgcg
     2041 cggtgatagc ccgctggtag gcagcaggcg tcatgctctc cggtcgcggc tcacgctcgg
     2101 tgaggaactg gctcaacggg tcatgcaagg cgcgatagac cgtcgcgagc ccgcggagaa
     2161 tgcccagata actcgcctca atctcctgtc ctcgaatggt ggccgggaca aaccagctgg
     2221 aggtggcgaa attctgatag cggctcgact tggcctggcc atcccacacc ggttcatcct
     2281 ccaaccggat ggcggcgagt tcggaaatct gctcgaagca gataatgacg tggccgaggt
     2341 ccgcgatcga accatgccca tagtcgaaat aaaactgttc ccagaatttc tcggatgaat
     2401 ggccgtggac ccacttgatg ctgtcttcaa tcgagtccgg agaccggcta taccgcgcga
     2461 gggcataggc ggatttctcc ggaggcatcg gagccagggc aatcacgcgg cgggctgaag
     2521 tcgtcatagg cttgaagtat gagaccgggc gtctggtgaa gcgaacgtac tgaggcggca
     2581 gcagcgccat gccattttta caaggagggc actatacgcc cgcagcgaaa agggtgcaac
     2641 agcgccgtcg ctcatgagat ggaaccgctc atcgcgttga cttgcccgca agcccgccgt
     2701 tatagtcccc gctcgaacga ccgcgaatcc acgattagag ccatgattaa ggccatcaaa
     2761 ggcgtcaaag atctccttcc ggaagagtcc ccccgttggc gattcatcga agataccgct
     2821 cggcgctggg cgctcaatta cggatttcag gaaattcgcg tccccatctt tgagaccacg
     2881 acattatttg cccgcagtat aggcgcgacg acggacatcg ttgagaagga gatgtatacc
     2941 ttcgcggatc gcgacggctc ctccctgacc ttgcgaccgg aagggacggc cggaacggtt
     3001 cgggcattca tcgaacacaa ccgagctgct gaccccaggc cgcagaaata ctgctatgcc
     3061 ggtcccatgt tccggcacga acggccacag gccgggcggc tccggcagtt tcatcagttc
     3121 ggggtggaat ccttcggcgt gtcggatcca cgggctgatg tcgaagtgct gtcgctcctg
     3181 tggagactgc tttctgatct caccctgccg gggttgacct tggaaatcaa caacctcgga
     3241 tacgccgacg atcgggcccg gtataaaccg cggctcgtgg cgtttttgaa gggtgttgaa
     3301 agccgtctct gcgggaattg tcagcgtcgc atcgaaacga atcccttgcg ggtcttagac
     3361 tgcaaggtgc cggaatgccg tgccgccacg gaagaggcac ctggcctggc tgattctctg
     3421 tcatcgccgg cccgtgagca ttttgatcat gtgaaggccg gcttgaccgc catcggtatt
     3481 cccttccact tgaatcctcg cctggtccgc ggtctcgatt actattgcct cacggctttc
     3541 gaggtgacct gctcgcactt aggggcgcaa aacgcagtgg gtgccggcgg acggtatgat
     3601 ggattggtca gagaactggg agggccaact gttcccgccg tggggttcgc cctcggtctt
     3661 gaacggatcg cgttgatgct gcccgagacc tttgtcgtac ccgccatccc gagagtctat
     3721 gtcgcggcct tcggtacaca ggccgcagat gccgggtttg tcctgctcga tgaacttcgt
     3781 cgggccggtg tcccggcgga tatggatttt cgctccgcat ccctcaaggc tcatctcagg
     3841 caggccgatc gccttggagc gctctatacc attctcctgg gtgatgatga agtcgcaaaa
     3901 ggaaccgcga ccgtccgcaa tatgcagacg aaagcacagg aagatcttcc tatccaggac
     3961 ctcgcaacgg catttcgaaa ccgacttctc aacaagtaat cccgctcagt ttctagttga
     4021 ctttccggcc caaaattcgt acgctccgcg tgcagtataa aacatataag taattgatta
     4081 ttaagaactc ctaaagtttt tccacaggca cattttttat gggcgcgctg aagatcggtc
     4141 tcctgttatc cacacccccg tcacacccca gtgtacagac ggtcgcctca ctagcgtccg
     4201 aagcgatcag ccagggggcc gacgtgtatc tgtattgcat cgatgaaggg gtgaagaatc
     4261 ttcgggaccc ccgatataca gaactcgtcg ggcgcggaat gaagttgttc gtctgtgcct
     4321 acgggtgtca gcagcatgga gtctcgacgg atcaactcga tccccgcatc tcactctgtg
     4381 ggttagtcgt gctttcgaat attgtgaatg gctgcgaccg atttctggcc ttcacctaat
     4441 gtgcctgagg gattgaattg tggccgcgac cgctaccatc gctttgatta ttcgggaaaa
     4501 tcctttgacc tccgctgttc cggtcgaagc acttcgcatt gccttagggt tggcggcggg
     4561 tgaaaatcca atcaccgtca ttctcatggg acctgcggtt catctcctgg ctgaagaatc
     4621 agacgacatt gtcgatctgg agatcctcga aaaatatctc ccctccttcg agcatctcca
     4681 gattcccttt attctcgtcc attcctccgg gccgcggccc gaccttcagg aaggatttgc
     4741 cgtcactgac gggacagtag aatccgcccg gcaggccatg gctgctgctg accgggtctt
     4801 ggtgttttag gaaggctgag ttgtatgtct gagcataaga ccctgtatct tcttcgccag
     4861 ccgatttcag atccggccga gacgctgtta ccgtctgcgg ccgagactca actggctgac
     4921 cgggtctctc tcgtacttct ggaagctgcc gcatcgacga ccccttcctt tcccggcccg
     4981 atctacgttc tccatcagga ctccgctaca ccggttcaag gtggttcccg aacacagatt
     5041 tcctatcgag agctggtcac cttgattgct gagcatcctt cgaccattgt gctgtgatct
     5101 ggatgctctt ctcttatata tgttctttat aaagagaaga tccggagccg tcggagtagg
     5161 agtaggacgt gtgggttctg tggatgagcg tgaaggactg cgcgggcatt ggcgatggaa
     5221 gatcctggcc catgttgatg agatggtgta taaggcaggg ggccttcagg gataacgtga
     5281 ggaggcctgt gcacgaggga tcgcgatgat tgtcggaatg attcgatgtc cacaggaacc
     5341 ctggaaagat tcccagcgcc gcatcaagcc aatcgcgtgt tcctatgtat ttgtccacac
     5401 ctgtgtataa gcctgtgaac atttgtaagt gattgaaata aaaagatgaa tcagatgtgg
     5461 aatgaggcgt tggagtacat tcaggagaag gtgccgaaac aggtctatga aacttggttc
     5521 accccggtgg tgctggaccg gattgaagag acgaccgctt atctcgccgt tccgaacaag
     5581 tttttcgggg attggctcgg ggagcattat cacgatctgc tcgcggaagc ggtatcggct
     5641 gcccagggtg gcgggcgtat ggaagtttcg tttgtggtca gtcagaaggc ggcacccgct
     5701 caggcgcagc cagatcactc ggccgcggat acggcgggac gaggattcat ggcctcgcgc
     5761 tccaagcgcg gcattcagct caatcccaaa tataccttca aaagtttcgt ggtcggcgcc
     5821 ggaaaccagt ttgcccacgc agcctgcatg gccgtggccg agcaaccggc gaaagcgtac
     5881 aacccgttat ttctgtatgg cggggtggga ttggggaaga ctcacctgtt gaatgcgatt
     5941 ggaaactatc tggctgagcg cagcgatctg cggatcgcct atttgacgac ggaacagttt
     6001 acgaacgagg tcatcaactc gattcgctac gataagatga tcgacctccg gaagcgctat
     6061 cgcaatgtcg atatgttgat gatcgacgac attcagtttt tggcgggcaa ggaacggacg
     6121 caggaagaat tcttccacac cttcaatacg ctgtatgaag cgcataaaca aatcgtcctc
     6181 tcgagtgacc gctttccgaa agacatgccc gatattgaag agcggcttcg gtcgcggttt
     6241 gaatgggggc tcatcgccga tctgcagccg ccggacgtcg aaacccggat cgccatcttg
     6301 aggaagaagt ccgaggatga acggattgcc cttcccgaag acgtgatcca ttttctggcg
     6361 aacacgatga agaacaacat ccgtgagttg gaggggtcgc tcgtgcgggt cggcgcctat
     6421 tcctcgctca cgggccaggc catcacgctg gagatggcga agaacgtctt gcgcgatctc
     6481 atcggtgaca aaaagaagat tgtctccatc gaagacatcc aggaagcggt gggatcgaaa
     6541 taccatgtga agattgccga tctgaaatcg cgccggcgga gcaaaacgct ggtgcatccc
     6601 cgccagatcg ccatgtatct ctgtcgggaa ctgaccgacg cctcgtttcc tgagatcggc
     6661 cgccagttcg gagggaaaga tcacaccacg atcattcacg cctgccggca gatcacgaaa
     6721 gccaaagagg ccgacagcgc gctgcacacg acgctcgaag gcttgaaaga acagatcttg
     6781 agggcgtgaa ggccctctga gggagaccga ccatgaaggt acgcatcgga cgagaggaat
     6841 tgttgacggg cctgcagcgc gt
//
LOCUS       DAOQDS010000116         6483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_7074_length_6483_cov_20.458774, whole genome shotgun sequence.
ACCESSION   DAOQDS010000116 DAOQDS010000000
VERSION     DAOQDS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7074_length_6483_cov_20.458774"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="PK589_17795"
     CDS             complement(<1..1452)
                     /locus_tag="PK589_17795"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNJ21520.1"
                     /translation="MPNHRIDLKRLARHAMIDRGLWPDFSAKAMAELAQIRAAAVDHG
                     ADAQDLRDLLWVSIDNDDSLDLDQITVATEHPDHSVTLLVAIADVDALVPKDSAIDTH
                     ARHNTTSVYTSGDIFPMLPEPLSHDLTSLREHQDRLAVVVDLAIAANGKVSGASHYRA
                     LVRNHAKLAYNSVAAWLTGTGPAPDSISDVPGLDAQLRLQDRVAQQLKARRHQQGALS
                     LETIEPRAVFDGDTLTGITVEQKNRAKELIEDCMIAANQATASYLKRKGIPSFRRILR
                     SPERWQRIVDVAASCGESLPPQPDAQALDAFLVKRRAADPIRFPDLSLAIVKLIGRGE
                     YVLDEGTGGAPEHFGLAVKGYTHSTAPNRRYPDLVTQRLLKAALAGAPLPYRTDELKY
                     LADYCTEKEDDAERVERQMRKSAAALLLQPRIGERFEAIVTGLSEKGTWVRLLEPPVE
                     GKLSGGVMGLDVGDRLTVELVSTNVERGFIDFSR"
     gene            complement(1621..1806)
                     /locus_tag="PK589_17800"
     CDS             complement(1621..1806)
                     /locus_tag="PK589_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21521.1"
                     /translation="MRFVKVKDEERPGEVAINLDLVREAHFGGGLLHLYFERSSSAQD
                     DMTFTGENAQKIWAAMG"
     gene            complement(1951..2232)
                     /locus_tag="PK589_17805"
     CDS             complement(1951..2232)
                     /locus_tag="PK589_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNJ21522.1"
                     /translation="MPYVLIIHEVDAYPAWKSIFDQAAQIRKQAGEIRYQLLQHDTNP
                     NRLVHFSEWTSLDKARSFFESPELVEIRRKAGVKAPAFHYLNEVEQGRL"
     gene            2427..2705
                     /locus_tag="PK589_17810"
     CDS             2427..2705
                     /locus_tag="PK589_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21523.1"
                     /translation="MANLFARHPHTVKDMIVAETLCPLHQEPVKVGFEMNGVQGKLTV
                     REHCSILQMGEKCSEACRSNPEILEAVHKKMAEFKEESAREIPIISTP"
     assembly_gap    2845..2944
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3201..3962
                     /locus_tag="PK589_17815"
     CDS             3201..3962
                     /locus_tag="PK589_17815"
                     /inference="COORDINATES: protein motif:HMM:NF018755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HNJ21524.1"
                     /translation="MPDPVQMQFSQANAYLTELISQFAGIEKRIEGGETVTLNLSDQG
                     RTLSLVVSREHVTESAPPAKSPTGGVSSMLADNHSLVAALTKTFVFDLGQANRNISAA
                     DVQRHFTHATRQTRRDEADLIPNVPDPVVKHDGHLWVVAEDCRYVALDGCIITAKRGF
                     KSDLASIPRLFWAVIASFELSLVAPIMHDLIYRCGGQVILPDGEVIPAGKLFSRKDAD
                     DVFLELMTRSKVAYWKRNVAYLAVRAFGQSSWQGR"
     gene            complement(4035..5234)
                     /locus_tag="PK589_17820"
     CDS             complement(4035..5234)
                     /locus_tag="PK589_17820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ21525.1"
                     /translation="MSVIIPTYNRAPLLKTAITSALHQERAGDLFDVEVIVVDDCSPE
                     DMSPIVSEFPGVRYIRLPENRGASGARNAGIKEASGKYVALLDDDDEFLTHKLMIQVP
                     VLEADPDVGVVYGQSVVTGSDTPLLLWPEWGPSGRVFEEFLTRTEDFLHPPTWLVRRE
                     LFEAAGWFNEQHRTMEHYDMALRLSMLTSWTFLSGGPVARGRFSKKGKWYSNIVNGTN
                     EERLPRIVESALARLPATPEAERTRRKARAAVCATIAGQRWWTGGGLEPTKQHLLASL
                     RTSAWLLHEAPVMEWIQRIAGTVAGGSHQPVQAVRAFWEEIKQATSAAPDAHTIPMRG
                     VLGDMLSSAAITMKQGSPRRAWMVGLGSIVHNPKSLKQPARLLHLYRALATTPATSGT
                     RLPISPS"
     gene            complement(5670..6128)
                     /locus_tag="PK589_17825"
     CDS             complement(5670..6128)
                     /locus_tag="PK589_17825"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNJ21526.1"
                     /translation="MHPLIVLAFIALAGGLLPIPAATFAQALPPALMQKIQAAQQHVN
                     TIGMEAYRKVTDNPGDALIIDVREPQEYAAGHVPGAVNIPRGVLEFQIWKHVGSPGAP
                     QMAKPLYLQCQSGNRASLAAQSLAELGFSQTTAVVMSLEEWQKAGHPFVK"
BASE COUNT         1304 a         1746 c         1936 g         1397 t
ORIGIN      
        1 tcgcgagaag tcgatgaaac cccgttcgac attcgtgctc accagttcga cggtcagacg
       61 atcgccgaca tccaacccca tcacgcctcc ggacagcttt ccttcgaccg ggggctcaag
      121 cagccgcacc catgttccct tctcggacag gccggtgaca atggcctcga atcgttcgcc
      181 gatacgaggc tgcaagagca aggcggccgc ggattttctc atttgccgtt ccacccgttc
      241 cgcatcatct tccttctcgg tgcaatagtc ggccaaatat ttcagttcat ccgtccggta
      301 gggcaggggc gcgcccgcca gggctgcttt gagcaatcgt tgggtcacga gatcgggata
      361 ccgccggttc ggcgccgtgg aatgcgtata ccctttcacg gctagtccga aatgttccgg
      421 cgcgccgccc gttccctcat cgagcacata ttcgcctcga ccgatcagtt tcacgatggc
      481 cagtgagagg tcgggaaacc ggatcgggtc ggccgctcgc cgtttgacga ggaaggcatc
      541 caatgcctgc gcatcaggct gcggaggcaa ggactcgcca caacttgcgg ccacgtcgac
      601 gatccgctgc cagcgttccg gggagcgaag gatgcgccgg aaggagggga tgcctttgcg
      661 tttgagatag gaagcggtcg cctggttggc ggcgatcata caatcttcga tcagctcctt
      721 cgcacgattt ttctgttcga ccgtgatgcc ggtcagagta tcgccgtcaa agacggcgcg
      781 tggctcgatg gtttcgagac tcagcgcacc ctgctggtgt cgtcgtgctt tgagctgttg
      841 cgccacgcga tcctgaaggc gcagttgcgc gtcgaggccc ggcacgtcgc tgatgctatc
      901 gggtgccggt cctgttcccg tcagccaggc ggcgacactg ttgtaggcaa gtttggcgtg
      961 attgcgaacc aacgcccgat aatgagacgc cccgctcact tttccattcg cggcgatggc
     1021 gaggtctacc acaacagcca gccggtcctg atgttcgcgg agcgaggtca gatcgtgaga
     1081 aagcggctca ggcagcatgg ggaaaatatc gcccgacgtg tagaccgagg tggtgttgtg
     1141 gcgcgcgtgg gtatcgatag cggaatcttt gggtacgagc gcatcgacgt cggcaatcgc
     1201 caccagcagg gtgactgagt gatctggatg ttcggtggcc accgtaattt gatccaggtc
     1261 gagcgagtca tcgttgtcga tggagaccca cagcaggtcc cggaggtctt gcgcgtccgc
     1321 tccatggtcg accgctgccg cgcggatctg ggccagctca gccatggctt tggcggagaa
     1381 gtctggccac aagccgcgat caatcatcgc gtgtcgggca agacgtttga ggtcgatacg
     1441 atgattcggc atgaggtcct cgagttgatg tgccaacagc aagccgtcga ggcgaatggc
     1501 ctccgtatga ctagctataa cacgaatcga gcagagcgtg ggcgtgagtc acagcctgcg
     1561 acagagggag gatgcaagga aaagggaatg ctggagaggt cgaggaatag ggccgtctcg
     1621 tcaccccatc gcggcccaga ttttttgggc gttctcgccg gtaaaggtca tgtcatcctg
     1681 cgccgaggaa ctgcgctcga agtacaggtg cagcaagccg cctccgaaat gcgcttctcg
     1741 caccaggtca agattgatgg caacttcacc gggccgttcc tcgtctttta cttttacgaa
     1801 tcgcatgatg acctcgttgt tggttgatgg cgctatcggt attcgcacag cgtgtgaagg
     1861 gtcagcatat cccagactga tgaaaagcgt gagcaaaggt cggaaatgtg tgtgaatacc
     1921 aggccgtcga ggcgactcag gtcgcggcat tcacaaccgg ccctgctcga cctcattcaa
     1981 atagtggaat gccggcgcct tgactcccgc tttgcgccga atctcgacca gctccggaga
     2041 ctcaaaaaag ctgcgggcct tgtcgagcga cgtccattct gagaaatgca cgagccggtt
     2101 gggattggtg tcatgttgca ggagctggta tctgatctca ccggcttgtt ttctgatctg
     2161 tgccgcctga tcgaaaatcg acttccacgc cggataggcg tccacttcat gaatgatcaa
     2221 cacgtaaggc atgagtctcc tcggttgtga cagccactga aggaaagtgt gattgcgtcc
     2281 agtctaatac agtgggggtc ggggatgcag ccgtggcgtg ggtacgctgc aaatgcgata
     2341 caagtttgtg ccggaggggt ctcttgatac agtgatgtgg gaggccactg taccactatt
     2401 cacccgtcac acagtaagga gtccatatgg cgaacctatt tgcaaggcat ccacatacag
     2461 tcaaggacat gattgtggcc gagaccctct gtccgctgca tcaggagccg gtgaaggtcg
     2521 gcttcgagat gaatggcgtg caggggaagt taacggtccg ggagcattgt tcgattctgc
     2581 agatgggcga aaagtgcagt gaagcctgcc gcagcaatcc tgaaatcctt gaggccgttc
     2641 acaagaagat ggcagagttt aaggaagaat ccgcgcgaga aattccgatt atttccactc
     2701 cctagtcccc tgtcggagtg acctgttctg gtggccctcg cgaactgtgg ccccagagag
     2761 ctttcagccg ccttcgcctg tgatgcgggg gcggcggttt ctccacctac cgccgcagtg
     2821 acgctcctct aggattcatt tcctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnggcggc ggtttctcca cctaccgccg cagtgacgct cctctaggat tcatttcctg
     3001 acatctcatt tcacgccttt cgcgtgtttc gtggcgcgcg atatctgttc gcttgcagat
     3061 gcctttcgat gtgtcagctg gatacccttt cctcacagaa attggccact tcgtgtgagg
     3121 ggaaggcttg aggaaggtct atcggtttag gcatgatgcc gcagtcgatg tgacggatgc
     3181 accaaaccga ggagacaggt atgcccgatc cggtacagat gcagttctcg caagcgaatg
     3241 cctatctcac tgagcttata agtcagtttg cgggtatcga gaagcgaatc gaaggaggcg
     3301 agaccgtgac cttgaacttg agtgaccagg gacgcaccct gtcgcttgta gtcagccgtg
     3361 agcatgtcac cgagagcgcc ccgccagcca agtctccaac gggaggtgtc tcctccatgc
     3421 ttgccgacaa ccatagcctt gttgccgcgc tgaccaaaac gtttgtcttt gatctcgggc
     3481 aggccaatag gaatatttct gctgccgatg tgcagcgtca ctttacacat gccacgcgac
     3541 aaaccaggag ggatgaagct gatctgatac ccaacgttcc tgatccggtt gtgaaacatg
     3601 atgggcacct gtgggtcgtg gcggaagatt gccggtatgt tgcgctcgat gggtgtatca
     3661 ttacggccaa gcgcggcttt aagagcgact tagccagtat cccgcgtctt ttttgggcgg
     3721 tcattgcgtc atttgagctc tcgctggtcg ctccgatcat gcatgatttg atctatcgct
     3781 gcggaggaca ggtgattctt ccggacggcg aggtgatccc tgccggcaag ctgttcagtc
     3841 ggaaagatgc cgatgacgtg ttcctggaat tgatgaccag atccaaggtc gcctattgga
     3901 aacgtaatgt ggcgtatctt gcggtgcgag catttggcca gtcatcctgg caagggagat
     3961 gaggtgaggc gggttttggt agggtctgca gtcggctctc ccgacggagg ccggcgaaac
     4021 aacgacctct caccttacga gggagaaatc ggaagtcgag tccccgatgt cgcgggagtc
     4081 gtggccagtg cgcgatacaa gtggagcagc cgtgccggtt gtttcagcga cttgggatta
     4141 tggacgatgg agccgagtcc caccatccat gctcgccgtg gtgacccttg tttcatggtg
     4201 atggccgcgc tcgagagcat gtctcccaac actccccgca tgggaatggt gtgagcgtcc
     4261 ggggcggcgg aggtggcttg tttgatttct tcccagaagg ctcgcaccgc ctgcaccggc
     4321 tgatgcgacc ctccggccac tgtgcccgcg atccgttgaa tccactccat caccggtgct
     4381 tcatgcagca gccacgctga ggtgcggagc gacgccagca gatgctgctt ggtcggttcc
     4441 agcccgccgc ccgtccacca tcgttggccg gcaatcgtgg cacagaccgc ggcgcgagct
     4501 ttgcggcgag tgcgttcggc ttcaggagtc gcgggtaatc gagccagggc gctctccaca
     4561 attcgcggga gccgttcctc gttggtgccg ttgacgatat tgctgtacca ctttcccttc
     4621 ttggaaaagc gaccacgcgc gaccggcccg ccgctgagaa atgtccagga ggtcagcatc
     4681 gacaggcgga gcgccatgtc gtaatgttcc atggttcgat gttgttcatt gaaccatcct
     4741 gccgcttcga atagctcccg tcgcacgagc caggtgggag ggtgaagaaa gtcttccgtc
     4801 cgcgtcagaa actcttcgaa cacccggcct gacggtcccc attccggcca caggagcagg
     4861 ggcgtatcgc tgcccgtgac gacactttga ccatagacga cgcccacgtc cggatcggcc
     4921 tccaggaccg gcacctggat catgagttta tgggtgagga attcgtcgtc atcatccaag
     4981 agggcgacat acttgccact ggcctctttg atgccggcat ttctggcacc cgatgccccg
     5041 cgattttcag gcaagcggat ataccgcacg cctggaaact cgctcacgat cgggctcatg
     5101 tcttccggtg agcagtcatc gaccacgatg acttccacat caaacagatc gccggctcgc
     5161 tcctggtgta atgcggaggt gatcgctgtc ttgagtagcg gtgcccgatt gtacgttgga
     5221 atgatgacac tcacgagcgg gcgtccggat gatcgcgtgg ccgccagtcg agacgctgtg
     5281 gggggctgag gtatcggcat gaattttgtc aaacaatcac tacagcggaa tgagaagaaa
     5341 cggctatggc ctgctgccta cccggcttgg atcggctcag catgaaaaca gcccgggtgt
     5401 ttgctcataa ttgataagca agcccggtgc cttattgaag ggttggctat cacggtgatt
     5461 cacccgatgg gcgggaagaa gatgtagacg cagggctgga cccttaggtc ctgaagctgg
     5521 aatgttcaac ctgataggtg cgcggcaaat ttttgaggga aagtcggcag actatcgatt
     5581 ccgcacagag cggagtgagc gaaaacgctc gagtccggag gttgctcagc gcggcagatg
     5641 cggtccgtgt cttctcgaca gggtatggat tacttcacga atggatgccc ggctttttgc
     5701 cactcctcaa ggctcatgac gacagccgtt gtctggctga atcccaattc cgcgagggat
     5761 tgcgccgcga gagacgcacg atttccactc tgacactgaa ggtacagggg ctttgccatt
     5821 tggggcgcgc caggggatcc gacatgtttc caaatctgaa attcaagaac gccacgggga
     5881 atattaacgg caccaggcac atgacccgcg gcatattcct gtggttcgcg tacatcaatg
     5941 atgagagcgt cgccaggatt atcagtcact ttccgataag cttccattcc aatcgtgttg
     6001 acgtgctgct gcgcggcctg aatcttctgc atgagggcgg ggggaagggc ttgcgcaaaa
     6061 gtcgcagctg gaataggcaa caggccaccg gcgagagcga tgaatgccag aacgatcagt
     6121 gggtgcatga catgctcctt tttatcgtga actagcgggg agcggacttt ccttggaact
     6181 ctggcacgag acgctactct acgcctctcg agggaaagcc tcaaattctg tgcatcgagg
     6241 agcccggtgt gttggaagga acttgagggg gagaaggggg ggtgatggca gtaccatcac
     6301 cccgggagac aacaggcatt catcggttga tatcagaatg gaggacacct tcgcacacga
     6361 acgatgcgct ctttcatcga agcacgcatg ctcggtagca gatgtccgtt ttcgctagat
     6421 gggctatccc tgtctgcaca aggactagcc gatgatcaca tggtctccat gatcatcgcc
     6481 ggg
//
LOCUS       DAOQDS010000117         6364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_7285_length_6364_cov_21.781265, whole genome shotgun sequence.
ACCESSION   DAOQDS010000117 DAOQDS010000000
VERSION     DAOQDS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7285_length_6364_cov_21.781265"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..340)
                     /locus_tag="PK589_17830"
     CDS             complement(59..340)
                     /locus_tag="PK589_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21527.1"
                     /translation="MPSTMVQVPILMSPGQKRRLAQKAKAANLTMAELLREGGERYVP
                     ADNPTLLDHMAKQVIRETKKTIRAIDKTLALVAESEARMLALSKTRKKG"
     gene            565..1026
                     /locus_tag="PK589_17835"
     CDS             565..1026
                     /locus_tag="PK589_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNJ21528.1"
                     /translation="MSIDEKLSSLGLTLPVAPKPVASYVPAVLAGDLLYLSGILPFRD
                     GQVAITGKLGQDVTVERGAEAARLALLNALAVVKQELGSLDRVQRIVRVVGHVASATG
                     FVQQPAVINGASDLLVQIFGEAGRHARVALGAAELPLHAAIELELLVQVKA"
     gene            1269..2942
                     /locus_tag="PK589_17840"
     CDS             1269..2942
                     /locus_tag="PK589_17840"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNJ21529.1"
                     /translation="MKYITCAILGVALVANSVDSKAAPAEPPALHPNAAVPGATLSIT
                     GKGFGPFKSTRFNQVTFQGVPALIQRWDSDLIEVRVPSQAANGPVDIIIGKKHVKAGS
                     FTRLQPVIHSVSPAEAEPGSMLEIAGEHFGNTAGPRDPNTIFGVNSVAVGDVSVRVRK
                     WRDDKIEVELPGNVQSGDVVIRMSSSDPLPDGSCCAPVKQVVSNAMPMKVLASVRVDP
                     SSGPVGTKVVLFGKGFGTTRNPEDGVLFGGHLATVSQWTDTTVVVHVPLDAQTGPVVM
                     KRNGQERAIGTYTVQTPKATALTPTEAPIGTLLKITGENFGFYSEAGSTPFNYIDFSL
                     SENTVEIGGVQAIVYRWGHDRIDVWVPFSAKSGPVVVKRAANTPKPDGTCCADKQVLE
                     TQVGNFTLVTPKIDSYSPNTGGLDEVVTIKGSGFGKFLKTAEPSKVITDSVYARVAPE
                     LGENVSRTEVLFNGVGAIVQSWTDTEIKVKIPHRHSYGVGKLGEFNPDLTSGPLVVRR
                     GSWDLLPDGSCCTPKKWVTLEAGTFTIQPSGLPDASYWRNPNPENSHFH"
     gene            2954..3925
                     /locus_tag="PK589_17845"
     CDS             2954..3925
                     /locus_tag="PK589_17845"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNJ21530.1"
                     /translation="MRTLAAVVCSLLVVFCVGTVEAGSPTVPVTVAMQKSGSEATLLG
                     MFFHDHQLGWAVGSGGTILKTTDGGHKWKKVSSGTSSLLTAVYFRDAKRGWVVGANGT
                     VRTSKDGGETWSVVVVSTQAPLYGIAFASPTKGWMVGGNGTILQTTDGGENWIDQASG
                     TSAALYAIALTNELQGTAVGALGTILTTADGGKNWTTQVSQSSATFFDVAFADDTNGW
                     AVGNAGALFQTIDGGTHWVDRTLPCGRTCNKLTDLIRVRFTDPQQGWIVGERGQILRT
                     SDAGFNWVEETSPVKRSLMALSFPRATAGWAAGEGGTIISISAAGNK"
     gene            complement(3948..4685)
                     /locus_tag="PK589_17850"
     CDS             complement(3948..4685)
                     /locus_tag="PK589_17850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNJ21531.1"
                     /translation="MPAFFISSAEIHGTALTLTGELCHHLRASLRVKPGEVLRLTDEH
                     RQRYEVRVSAVAPQALTAEILESRPGPIDTSPRVILAQVILKGDHMDWVVQKASELGV
                     KAVLPLISRHGVVRPQPERVAAQVARWQRIATEAAQQSEQWQPPQVLEPLEVRRFFSG
                     HQATTALLLAERQNAVGLANVPLPALPTDTITVIVGPEGGWAEEELSQAMDGQCLAVS
                     LGEHILRADTAAVTALSIVQSRLGRLG"
     gene            complement(4722..5834)
                     /gene="dnaJ"
                     /locus_tag="PK589_17855"
     CDS             complement(4722..5834)
                     /gene="dnaJ"
                     /locus_tag="PK589_17855"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNJ21532.1"
                     /translation="MAKRDYYETLGIERNATDDEVKKAFRKLARQHHPDLHTSPEQKK
                     SAEEKFKELNEAYEVISDPDKRRRYDTFGHAGGPQGAEGFDFGGQGGFGDIFNDIFED
                     FFGQPRGRARAERGNDLQYNLEITFEESVFGKEAKLKIPRWETCADCKGTGAKSATAI
                     KMCPACKGQGQLRFQQGFFSVSRPCGQCEGVGQIITEPCQVCGGRQRIRKERMLSVNI
                     PGGIETGMRLRLANEGEHGVQGGPQGDLYVAVTVKPHPHFRREGQDIACDLPINLVTA
                     ILGGRVEVPTLKGNTFMKIPAGTQPDKILRLKGLGFPNLKGGHTGDQLFNVKVEIPTK
                     LTSRQKELLEEFAKESGYTKDSEGEGFLDKMKTFFE"
     gene            complement(5915..>6364)
                     /locus_tag="PK589_17860"
     CDS             complement(5915..>6364)
                     /locus_tag="PK589_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNJ21533.1"
                     /translation="QSIKITASSGLSKEEVDKLVKDAQSHTEEDKKRRRVAEARNQAD
                     SLLYSTEKNLTEHGDKIGEDDKTKITEAIAGLRKAMEGDDPATIESATQALTTASHKL
                     AEEMYKKASASAGPTDAGASAGDGGAQAKTDEKVVDAEFEEVDKDKK"
BASE COUNT         1190 a         1744 c         1952 g         1478 t
ORIGIN      
        1 tcgttcagct tgatggtgtt ggcgaaggca tcccacacgc gttcggtgag tcccatcgtc
       61 accctttctt acgggtcttg gagagagcca gcatgcgcgc ttcggactct gccacgaggg
      121 cgagagtttt gtcgatggcg cgaatggttt tctttgtctc ccggatgact tgcttggcca
      181 tgtgatccaa gagtgtcgga ttgtcggctg gaacgtaccg ctcgccccct tctcgaagta
      241 gctctgccat ggtgaggttg gcggctttcg ccttttgtgc cagccggcgt ttttggccgg
      301 ggctcatgag gataggaact tggaccatcg tggatggcat ggaggcctcc ctcggcgtgg
      361 atatatagcc catatatatc ataggatcgg atatgctgtc gatgcgcccc atgtcctcag
      421 cggtttatga tgcatgctgg agggggtcat cttgctcaat cgccctcgga tgacgtatac
      481 gtagggctca atgttcgagt tgcactacgt gggctcttgc atacctgacc ttcggtgggg
      541 tgctgaacag gaaaggatca taccgtgtcg attgacgaga aactcagcag ccttggcctc
      601 acacttccgg ttgcgccgaa gccggttgcc agttatgtgc cggcggtgct tgccggtgat
      661 ttgctgtatc tgagcggtat cttgccgttt cgggacgggc aggtagccat tacaggcaag
      721 ctgggacagg acgtaacggt tgagcgggga gccgaggcgg ctcgattggc gctcctgaac
      781 gccttggcgg tcgtgaaaca ggaactgggg tcgttggacc gagtgcagcg tatcgtgcga
      841 gtcgtgggcc atgtcgcctc ggccaccggc tttgtgcaac agcctgccgt gatcaatggg
      901 gcatcggatt tattggtgca gattttcggt gaggcgggac gccatgcacg tgtggcgctt
      961 ggcgcggcgg agttgccatt gcatgccgcc atcgaactcg aactactggt tcaagtaaaa
     1021 gcctgacgta cccgttctgc gctgtccttt ccctcgtcgt tgcttgcctt gacatcaaaa
     1081 aaaaccgctt gttatagtcc ggaagtcttc tggtttgata ccacaccccc catccggctt
     1141 cgttgcctca gtggcgtggg gttttgtgcc acgtgcgggg cgttcatctg gcgtaccatc
     1201 attcatttca tcctcacccg gcctgctcgt caggctcatt caaagggtat cggtagggag
     1261 tggatcccat gaaatatata acttgtgcca tcctcggcgt agccttagtg gcgaattccg
     1321 ttgactccaa ggcagccccg gctgaacctc cggctctgca tcccaatgcg gccgtgccgg
     1381 gtgccacact gtcgatcacg ggcaaagggt tcggtccctt caaatcgacc cggttcaatc
     1441 aggtgacgtt tcaaggcgtg cctgccctga ttcagcggtg ggattcggat ctcatcgagg
     1501 tgcgggtgcc gtcgcaggcc gccaatggcc cggtcgatat catcatcggt aagaagcatg
     1561 tgaaagccgg ttcgttcacc aggttgcagc ctgtgattca ctccgtatca ccggctgaag
     1621 cagaaccggg ctcgatgtta gaaattgccg gagagcattt cggcaacact gcgggcccgc
     1681 gcgatccgaa tacgatcttc ggcgtcaata gcgtggccgt cggcgatgtg agcgtccgcg
     1741 tgcgcaagtg gcgtgacgac aaaattgaag tcgagttgcc ggggaatgtg cagtcgggcg
     1801 atgtggtgat tcgcatgtcc tcatccgatc ccttgccgga cggatcgtgt tgtgctccgg
     1861 tcaagcaagt ggtgagcaat gcgatgccga tgaaggtcct ggcgtctgtg cgggtcgatc
     1921 cttcgagcgg cccggtcggc accaaagtgg tgctgtttgg caagggcttc ggaaccacca
     1981 gaaatcctga ggatggcgta ctctttggcg ggcatttggc cacggtgtcg cagtggacgg
     2041 atacgaccgt cgtggtccat gtgccgctcg acgctcaaac cggtcctgtt gtgatgaagc
     2101 gtaacgggca ggagcgcgcc atcggaacat ataccgtgca gacacccaaa gcgacggcgc
     2161 tgacgccgac ggaggcgcct atcggtacgt tgttgaaaat tacaggagag aatttcggat
     2221 tttattcgga ggccgggtcg acaccgttta actatatcga tttttcattg agcgaaaaca
     2281 ccgtcgagat cggcggtgtg caggcgattg tgtatcgatg gggacatgac cgcattgatg
     2341 tctgggtgcc cttcagcgcg aagagcgggc cggtggtggt gaagcgcgcc gccaacacgc
     2401 cgaagccgga tggcacctgc tgtgctgata aacaggtgct ggagacgcag gtcggtaact
     2461 ttacccttgt gacgccgaaa atcgattcgt acagtccgaa caccggggga ctcgatgaag
     2521 tcgtgaccat caaggggagc gggttcggta agtttttgaa gacagccgag ccgagcaagg
     2581 tcatcacgga cagtgtgtat gcccgcgtgg ccccggagtt gggtgaaaat gtctcgcgta
     2641 ccgaggtctt gttcaatggg gtcggagcga ttgtccagtc ctggacggat acggagatca
     2701 aggtcaaaat ccctcatcgt cactcctacg gagtagggaa acttggggaa ttcaatcctg
     2761 acctgacatc cgggccgctg gtcgtgcgga gagggtcgtg ggatttgcta ccggatggtt
     2821 cttgctgcac gcccaaaaaa tgggtcacgt tggaggccgg cacctttaca attcaaccgt
     2881 ccggtctgcc ggatgccagt tattggcgaa atccgaatcc tgaaaacagt cactttcatt
     2941 gaggcacttt acgttgcgta ctttggctgc agtggtgtgt tcgctcttgg tcgtgttctg
     3001 cgtcggcacg gtcgaggctg gttctcctac cgttccggtg accgtcgcga tgcagaaaag
     3061 cggctccgaa gcgacgctat taggcatgtt ttttcacgac caccagctcg ggtgggccgt
     3121 tggatcaggt gggacgattc tcaagactac cgacggtggc cacaagtgga agaaggtctc
     3181 cagcggcacg tcgtcactcc tgaccgccgt ttactttcga gatgcgaagc gcggatgggt
     3241 cgtgggggcg aatggcacgg tcagaaccag caaggatggt ggcgagacgt ggagtgtcgt
     3301 cgttgtgtcc actcaggcgc ctttgtacgg gattgccttt gcatctccga cgaagggctg
     3361 gatggtgggt gggaacggca ctattttgca gaccacggac ggcggagaga actggatcga
     3421 ccaggccagc ggcacgagcg cggcgttgta tgccattgcg ctgaccaacg aattgcaagg
     3481 cacagccgtc ggcgcgttgg gcacgattct cacaacggcg gatggcggaa agaactggac
     3541 cacacaggtc agccaaagct cggcgacctt ttttgatgta gcctttgcgg atgataccaa
     3601 cggatgggcg gtcggcaatg ccggggcgtt attccagacg atcgatgggg gaacccattg
     3661 ggtcgatcgc accttgccct gtggccggac ctgcaacaaa ctcacggatc tcattcgtgt
     3721 acggttcacg gatccgcagc aggggtggat cgttggggaa cgcggccaga tcctgcgaac
     3781 cagcgatgcc gggttcaact gggtggaaga gaccagtccg gtgaaacgtt ctctgatggc
     3841 gctcagtttt cctcgtgcca cggccggctg ggccgccggc gagggaggga ccatcatttc
     3901 catcagcgca gcaggcaata agtaaccact cgccgcctgt ctaccgtcta acctaatcgt
     3961 cccaatcgac tctgcacgat acttaaggct gtgaccgccg ccgtatccgc tcgaaggatg
     4021 tgctccccca gacttacggc caggcactgt ccgtccatag cctgagacag ttcctcctcc
     4081 gcccatccgc cttcagggcc aacaatcaca gtgatggtat cggttggaag agccggtagg
     4141 ggaacgttgg ccaaccccac ggcgttctgc cgctccgcta acagaagtgc tgtggtggct
     4201 tgatggccgg agaaaaagcg gcgaacttct aacggttcca atacttgtgg cggctgccat
     4261 tgttccgact gctgtgcggc ttcggtggca atccgctgcc agcgagccac ctgggccgcc
     4321 actctctctg gttgcggtcg tacgacgcca tgccgtgaga tcagcgggag aacagccttc
     4381 acgcccaact cgctcgcctt ctgcacgacc caatccatat gatcgccctt gagtatgact
     4441 tgcgcgagga tgactcgtgg cgacgtatcg atcggcccgg gacgagattc gagaatctcg
     4501 gcggtcaacg cctgcggagc caccgcgctc acgcgcacct catatcgctg acggtgttca
     4561 tcggtgagtc gcaggacctc gccaggctta acgcgcaagc tggcacgcag atggtggcag
     4621 agttcgccgg taagggtgag tgcggttccg tggatctcgg ctgaggagat aaaaaatgcc
     4681 ggcatgacgg tcggcggatc tggaaggtgc agaagcccgg tctattcgaa aaacgtcttc
     4741 attttgtcta aaaagccttc gccctcgcta tccttcgtgt agccgctttc cttggcgaat
     4801 tcttccagca gttccttctg ccgcgaggtg agctttgtgg gaatctccac tttcacattg
     4861 aacagttgat ccccggtatg gccacccttg aggttcggga atccgagccc cttgagtcgc
     4921 agtattttat caggctgcgt gcctgcagga atcttcatga acgtgttgcc cttgagcgtg
     4981 ggcacttcaa cacggccgcc gaggatggcc gtcacgagat tgatgggaag gtcacaggca
     5041 atatcctgcc cttctcggcg gaagtgggga tggggtttga ccgtcacggc gacatagagg
     5101 tcgccctgcg ggccgccttg cacaccatgc tccccttcat tggcgagccg caggcgcatg
     5161 ccggtttcga taccgcctgg aatattgacc gacagcatgc gttccttgcg gatgcgctga
     5221 cgtccaccgc agacctggca cggttcggtg atgatttgtc cgacgccttc gcactgaccg
     5281 cacgggcggc tgacgctgaa gaatccttgt tggaaacgca actgtccctg tcctttacag
     5341 gcaggacaca tcttgatggc ggtcgcggat tttgcgccgg tgcctttaca gtccgcacag
     5401 gtttcccagc gaggaatttt gagcttggct tccttgccga agaccgactc ttcgaaggtg
     5461 atctcgagat tgtattgaag gtcattcccg cgctcggccc gggcacggcc acgaggctga
     5521 ccaaagaagt cttcgaaaat gtcgttgaag atatcgccga atccaccctg gccgccgaaa
     5581 tcgaatccct cggcgccctg cggtccgccg gcgtggccga atgtatcgta ccggcgacgt
     5641 ttgtccggat cgctgatgac ctcgtaggct tcgttcagct ctttgaattt ctcttcggcc
     5701 gatttcttct gctcgggcga ggtatgcagg tccgggtgat gttggcgagc gagttttctg
     5761 aaggcctttt tgacttcgtc gtcggtcgcg ttccgttcga taccgagggt ttcgtaataa
     5821 tcgcgttttg ccacggaaga gggttacccg ttctaagtgc aacaggacca agcctcaccg
     5881 tccgtgaagc ctggtcctgc ctgcagtgag gagcttactt cttatcctta tcgacttctt
     5941 cgaattccgc gtccacgacc ttttcgtcgg tcttggcctg cgccccacca tctccggctg
     6001 aagcgccggc atcggtagga ccagcggaag ctgaagcctt tttgtacatt tcttcggcca
     6061 gtttatggga ggccgtggtc aatgcttgcg tggccgattc gatggtggcg ggatcatccc
     6121 cttccatcgc cttacggagg ccggcaatgg cttcggtgat cttggtcttg tcgtcttcgc
     6181 caatcttatc gccatgttcg gtcaggttct tctcggtgct gtagagcaga gaatctgcct
     6241 gattgcgggc ttcagcgacc cgacggcgtt tcttgtcttc ttcagtatgg gactgcgcgt
     6301 ccttgaccag cttgtccact tcctctttgc tgagtccgct ggacgccgtg atcttgatgg
     6361 actg
//
LOCUS       DAOQDS010000118         6223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_7561_length_6223_cov_21.447147, whole genome shotgun sequence.
ACCESSION   DAOQDS010000118 DAOQDS010000000
VERSION     DAOQDS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7561_length_6223_cov_21.447147"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PK589_17865"
     CDS             complement(<1..764)
                     /locus_tag="PK589_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNJ21534.1"
                     /translation="MPQVLVLGSGKIGSLIAGLLSAKGQYQVHLADLTLDAPKRLIDD
                     LALTTVTPCALDVRRPDSVVAYVTAHRFDAVISCLPYFYNPAVAEIARAHHLHYFDLT
                     EDVAVTGCVKRISEGADHAFLPQCGLAPGFISIVTNDLIGHFESIDHVKMRVGALPVH
                     PSNALKYSLTWSTDGLLNEYGNVCVGIERGEEVHLRPLEGYETIELDGLLYEAFNTSG
                     GLGTLADTYRGRVRTMNYKTLRYPGHCEKIQFLMNDL"
     gene            complement(884..1669)
                     /gene="ricT"
                     /locus_tag="PK589_17870"
     CDS             complement(884..1669)
                     /gene="ricT"
                     /locus_tag="PK589_17870"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNJ21535.1"
                     /translation="MNDRMVYHPAMVDKLLAQELAKPYPSVIRIVGVKIRDRGEVKKF
                     DAGEATLAVGDRVLVEVGGELSYGVVYGAPQATPFIPPMRVIQSIARKATAEDAATID
                     RYERLASEGMRACREQAVALGLKMKLVEVFCSFHRRQMTFVYTAEDRIDFRELVRLLA
                     RRFGGRIEMRQVGVRDEASRLGGIDTCGLVLCCAAFLTEVKPVTVKQARVMGLPVDDP
                     KLLGVCGRLKCCLLFEAMESQPSSSSSKSSALINPARTRLASS"
     gene            1865..2110
                     /locus_tag="PK589_17875"
     CDS             1865..2110
                     /locus_tag="PK589_17875"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HNJ21536.1"
                     /translation="MRIPNKVVLPFGYHIMIRQVTDSEMDRQDANADGIWDNEAKTIY
                     IRKRLPVTRRRYILAHELGHAWLDWQHRYLDDGKARS"
     gene            complement(2167..2649)
                     /locus_tag="PK589_17880"
     CDS             complement(2167..2649)
                     /locus_tag="PK589_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNJ21537.1"
                     /translation="MAEGRHYWLMKSEPNVFSLDDLARAPKQITSWDGVRNYQARNFM
                     RAMKVGDQVLFYHSNAQPPCVMGIAEVVKTAYPDATQFDPHDVHYDPASPADQPRWDV
                     VDIRFVRKFAAPLSLDLLREQVRLKGMELLRKGSRLSVQPVKPSEWDEILRLARTHRD
                     "
     gene            2741..3559
                     /locus_tag="PK589_17885"
     CDS             2741..3559
                     /locus_tag="PK589_17885"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNJ21538.1"
                     /translation="MDLTPMWFGVYKASKYLLYPLSWIVLSGLLTLLIASLPVTPRRT
                     TWVRRLAFSTVLLLFLTATPLLSHLYISQLEARYPPFAAPPVAKFDAVVVLAGGVFHK
                     GSLRPADETSDPSRQRTACGAELWFQGLAPKMVLAGGDATVLRTGPPESREMKRWAIR
                     LGVPESVIHVEENSRTTYENAVQTRALLGPAHILLVTAAYHLPRAVGLFKKQGFTVTP
                     VACGYESTHHPQQAWEEKTVFDFIPTARALLLSTQTIDEVAGILVYWITGKWQP"
     gene            complement(3592..>3957)
                     /locus_tag="PK589_17890"
     CDS             complement(3592..>3957)
                     /locus_tag="PK589_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21539.1"
                     /translation="VSGHIIGLLKEYMRDLVEQARQESQAQQTFGFSAPPYQPDHAIA
                     DLLSILDDRIESEGLQVGMPLEFLHEMWRHCNEARSQVMEQVWLESNLGPPGSSKDRI
                     RELTYAALITHLESDAQQE"
     assembly_gap    3958..4057
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4058..4672
                     /locus_tag="PK589_17895"
     CDS             <4058..4672
                     /locus_tag="PK589_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNJ21540.1"
                     /translation="TVSCQKGCAACCRMLVPISAPEAFALAMSIDRLAPPERERLLSK
                     IDVAQQRLARAGILQQLSALAESSDPISDEAIEPLNRAYYALRMPCPFLDNEACSIYD
                     DRPAACRELAVTSPAADCQDMTNQTIRPVPVAVRISTALSLLWADLTATTPRLIPLPL
                     AIDWAKRHQAEQARQWAGTELFEKAIDKIGRYLGQERARRGSGG"
     gene            4763..5383
                     /gene="thrH"
                     /locus_tag="PK589_17900"
     CDS             4763..5383
                     /gene="thrH"
                     /locus_tag="PK589_17900"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HNJ21541.1"
                     /translation="MQNPVIVCLDLEGVLVPEIWVNFAIKTGIEELKITTREMPDYDA
                     LMTRRLNILDQHGYTLADIQSVIDAMGPMEGAAEFIAWLRERAQVIILSDTFYEFALP
                     LMRQLGFPTIFCNQLEIGPNGRIVSYKLRQPNQKKHAVAALKSLNFRVMAAGDAYNDT
                     AMLGEAHAGFFFRPPAHLPKEFPQFPVTTTYAELQEQFRKAGGLKA"
     gene            complement(5427..>6223)
                     /locus_tag="PK589_17905"
     CDS             complement(5427..>6223)
                     /locus_tag="PK589_17905"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNJ21542.1"
                     /translation="ARVERMKTLLQDLLDYVAGATCRQLAILDYFSDEVERALGPCGL
                     CDRCKTPVQLPVDVVNDESACAKAILTAVSWCGGRFGVTRIVEMLRGSRSKGLLAYGA
                     EGYPGYGIYQTWTKTALTRLVKSLIDTGHLQVEGSEYPTLDLTRRGQEVVTGLRPVEV
                     KTAVQSPASSAYQTPVNGPNNSMAASLTSSVDPQLFERLRQLRKELAEEEGVAPFVIF
                     HDKTLRTIAGHKPVTLSALLEIPGIGEVKVERYGRRVLGIVNEECR"
BASE COUNT         1376 a         1870 c         1622 g         1255 t
ORIGIN      
        1 agatcgttca tgagaaattg aattttttcg caatggccgg ggtaccgcag ggtcttgtag
       61 ttcatcgtcc ggacccggcc gcgataggtg tccgcgagag tgcccagccc acccgacgta
      121 ttgaaggctt catagagcag accatccaat tcaatcgtct cgtacccttc caaaggccgc
      181 aggtgaacct cttccccccg ttcaattccg acacagacat ttccatattc attgagcaac
      241 ccgtcggtcg accacgtcag ggagtacttc aaggcattac tcgggtggac cggcaatgcg
      301 cccacgcgca tcttcacatg gtcgatggat tcgaaatggc cgatgagatc attcgtgacg
      361 atgctgataa aaccgggagc caacccgcac tgagggagaa acgcgtgatc agcgccttcg
      421 ctgatcctct tgacgcatcc ggttacggcc acgtcttcgg tgaggtcaaa atagtggaga
      481 tgatgggctc gcgcaatttc agcgaccgcc ggattataaa aatacgggag acaggagatc
      541 accgcgtcaa aacggtgggc agtcacatag gcaacgaccg aatcaggccg ccgcacatcc
      601 agcgcgcagg gagtaaccgt cgtcagcgcc aggtcatcga tcaatcgttt gggcgcatcc
      661 agcgtgagat cggccagatg cacctgatac tgtcccttgg ccgacagcag cccggcaatg
      721 agagagccga ttttcccgga tccgagtacc agtacctgag gcatccgtca cctcatggtc
      781 attatactct ttcatactac aatgacgagg ctttcaccag caggtcgcaa aagcaggctt
      841 cgcagcgcga tgcgacagag atctggccga aacggatggg gaactaggaa gacgccaaac
      901 gggtacgagc gggatttatc agtgcagacg atttggatga cgaggaggac ggctgacttt
      961 ccatagcttc gaacaagaga caacacttga ggcggccaca gactcccagt aatttggggt
     1021 catccacggg aagacccatg acgcgggctt gtttcacggt caccggtttg acctctgtca
     1081 gaaatgcggc acagcacaag acgagcccgc acgtgtcgat tccgcccaat cggctggcct
     1141 catccctaac gcccacctga cgcatttcga tccgcccgcc gaaccggcgc gcgagcagac
     1201 gaactaactc gcggaaatcg atacgatctt cggccgtata gacgaaggtc atctgccgcc
     1261 gatgaaagga gcagaagact tcgaccagtt tcatcttcag ccccaacgcg acggcctgtt
     1321 cccggcaggc tctcatgccc tcggaagcca gtcgttcata gcgatcaatg gttgccgcgt
     1381 cctccgcggt ggctttccgg gcaatggact gtatgacacg catgggcggg ataaatggcg
     1441 ttgcctgagg agccccgtac acgactccgt agctcagctc acccccgacc tcgaccaata
     1501 cgcgatcgcc tacggctaag gtggcttccc cggcgtcgaa tttcttgact tcgccgcgat
     1561 cacggatttt gacacccaca atccggatga ccgacgggta aggcttggca agttcttgag
     1621 cgaggagttt atccaccatg gccggatgat acaccattcg atcgttcaat tgaaatcctc
     1681 cacaccctct ttacacctga agacaccagg ggagcggaaa acacaccagg cggaataacg
     1741 acgcataagc acctgaaaaa actgaatttt taatcgggga agacgaaaca ggaaatatgt
     1801 taggctatcc gggagcagga atccggcgca gaaggcgccc ttaagaaaga cagtgagggg
     1861 cacggtgcga ataccaaaca aagtggtcct tccattcggc tatcacatca tgatccggca
     1921 ggtcacggat tcggagatgg accggcagga tgccaatgcg gatgggattt gggacaacga
     1981 agcaaaaacc atttatatcc gcaagcggct tcccgttacg cgacggcgtt acatacttgc
     2041 ccacgagctt ggccatgcct ggctcgactg gcagcatcgc tatttggatg acggaaaggc
     2101 tcgcagttag ccgttccttt acgaatgaag atcttgaagg ggtccattcg cccctcacgt
     2161 accgcttcag tcacgatgag tacgcgcgag tcgaaggatt tcatcccact cagacggctt
     2221 caccggctgt accgacaggc gcgatccctt tcgaagcaat tccatcccct ttaaacgtac
     2281 ctgttctctg agcaaatcca atgagagtgg cgcggcaaac ttccgcacga accggatatc
     2341 caccacatcc cagcggggct ggtcggctgg gctggccgga tcataatgca catcgtgggg
     2401 atcgaactgt gtcgcatcag gataggccgt tttgaccact tcggcgatcc ccatcacgca
     2461 aggcggctga gcgttgctgt gataaaacaa cacctggtca ccgaccttca tcgcccgcat
     2521 gaaattacgc gcttgatagt tccgcacccc gtcccaagac gttatctgct tcggcgcgcg
     2581 tgccagatcg tcgagtgaaa agacgttggg ttcggatttc atcaaccagt agtgccgtcc
     2641 ctctgccata ccatcctcct gaacacttgg tgtccgtcac acctccgtgt atgctgagca
     2701 ttctttgagc caaattcttt gagccaaccg tggagttcct atggatctga ccccgatgtg
     2761 gttcggtgtc tataaagcct caaaatatct tctgtatccc ttgtcctgga tcgtgctgtc
     2821 cggactcttg accctcctca tcgcctctct gcctgtcacg cccagacgaa cgacctgggt
     2881 gagacgcttg gccttcagca cagtgctgct cctcttcctt actgccactc cccttctttc
     2941 acacctctat atttcgcaac ttgaggcgcg ctatccgccg ttcgcagcac cccccgtagc
     3001 gaaattcgac gccgtggtcg tcctcgcagg gggtgtcttt cacaaaggat cattgcggcc
     3061 ggccgacgaa acctccgacc cctcgcgcca gcgaaccgct tgcggcgccg aattgtggtt
     3121 tcagggcctc gcacccaaga tggtgctcgc cggcggcgat gcaaccgtac tccgaaccgg
     3181 accgcctgaa tcccgcgaga tgaaacggtg ggcgatccgc cttggtgtgc cggaatcggt
     3241 catccatgtg gaggagaatt cccgaacgac ctacgaaaac gcggtgcaga ccagggccct
     3301 cctgggcccg gcccacatcc tgctcgtcac cgctgcctat catctccccc gcgcggtggg
     3361 cttatttaag aagcagggct ttacggtcac tcccgttgcc tgcggctacg aatccacgca
     3421 ccatcctcaa caggcctggg aagagaaaac agtgttcgac ttcatcccca cggcccgggc
     3481 actcttactc agcacgcaga ccatcgacga agtggccggc attctggtct attggatcac
     3541 cggaaaatgg caaccgtgag tgtcctcggc acgaatgacg accgggcacg gctattcttg
     3601 ttgggcatcc gattcgagat gggtgatcaa tgcggcgtag gtcaactctc gaatcctatc
     3661 cttggatgaa cccggcgggc caagattcga ttccagccac acctgttcca tcacctggct
     3721 gcgggcctcg ttacaatgcc gccacatttc atgcagaaac tcaagcggca tgccgacttg
     3781 cagaccttcc gactcaatcc gatcatctaa aatcgagagc aggtccgcga tggcatgatc
     3841 cggctgatac ggcggtgcgg aaaagccgaa cgtttgctga gcctgcgact cctgccgcgc
     3901 ctgctcgaca agatcacgca tatattcttt taacaaaccg atgatatggc cggatacnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnact gtttcctgcc agaagggctg
     4081 cgcggcctgt tgtcgcatgc tggtgccgat ttccgcgccc gaagccttcg ctctcgcgat
     4141 gagcatcgat cgcctggccc caccggagcg tgagcggctc ctgtcaaaaa ttgacgtggc
     4201 tcagcaacgc ctggcccgcg ccggaatcct gcaacaactc tccgcactcg ctgaatcttc
     4261 agacccgatc agtgacgaag ccatcgaacc cctgaaccgg gcctattatg cgcttcgaat
     4321 gccctgccct tttctagaca atgaggcctg cagcatctac gatgatcgac cggcagcctg
     4381 ccgggaactc gccgtgacct ccccggctgc cgactgtcag gacatgacga atcagacaat
     4441 ccggccggtt cccgttgcgg ttcgcatcag cacggcgctg agtttactct gggcggatct
     4501 cacggccacc acacccaggc tgatccccct gccgctggcc atcgactggg ccaaacgcca
     4561 tcaggccgaa caggcccgcc aatgggccgg aaccgaacta tttgaaaagg cgatcgataa
     4621 aatcgggcgg tatctcggtc aggaaagggc tcggcgcgga agcggcggat aacagacaga
     4681 tttgggggtt cttcgctgtg acgtgtgcga gcgctccctc gtcccatcac tattcacatg
     4741 tacgactttt ccggaggaat gaatgcagaa tcccgttatt gtgtgtctcg accttgaagg
     4801 cgtcttagtt cctgaaatct gggtgaactt tgccattaag accggcattg aagaactcaa
     4861 gatcaccaca cgggaaatgc cggactatga tgccttgatg acgcgccggt tgaacattct
     4921 tgatcaacat ggctacaccc tcgccgacat tcaatcggtc atcgatgcca tggggccgat
     4981 ggaaggggcc gccgaattta tcgcctggct gcgcgaacgc gctcaggtca tcatcctgtc
     5041 tgacacgttt tatgaattcg cccttccgct gatgcgccaa ctgggctttc caaccatttt
     5101 ttgcaatcaa ctggaaatcg gccccaacgg ccgcattgtc agctacaaac tccggcaacc
     5161 caaccagaag aaacacgcgg tggctgcact gaagagcctg aatttccgcg tcatggccgc
     5221 aggggatgcc tacaacgata ccgccatgct gggagaggcc catgccggat tcttctttcg
     5281 tcccccggct catctgccaa aagaattccc gcaatttcct gtgacgacga catatgcgga
     5341 attgcaggag caattccgta aggcgggagg gctgaaggct taataagaac gcagaatgaa
     5401 acgcggacga tacaacacgg aagaagttac cgacactcct cgttgacgat cccgagcacg
     5461 cgccggccat accgctccac cttaacctcg ccgatgccgg ggatctccag cagggcgctc
     5521 aacgtcacag gtttatggcc ggcgatcgtg cgaagcgttt tgtcgtgaaa aatcacgaag
     5581 ggcgccacgc cctcttcctc ggcgagttct ttacgcaatt gacggagccg ttcaaacaac
     5641 tgcggatcaa cggacgacgt gagtgatgcg gccatggagt tgtttgggcc gttgaccggc
     5701 gtctgatacg ccgacgaagc cggggactgc accgccgtct tcacttctac aggtctgagg
     5761 cccgtcacca cctcctgccc gcgccgcgtc aagtcgagcg tgggatattc cgacccttcc
     5821 acctgcaaat gtccggtgtc gattaaggat ttcaccagac gagtcagtgc cgtcttggtc
     5881 caggtctgat agattccata tcccggataa ccttcagccc cataggccag gagccctttc
     5941 gagcggctgc cgcgcagcat ctccacaatc cgcgtaactc cgaatcgccc cccgcaccag
     6001 gagaccgccg tgagaatggc cttggcacat gccgattcat catttacgac gtccacaggc
     6061 aactgcactg gcgttttgca acggtcacac aatccacaag ggcccagcgc ccgctccacc
     6121 tcatcactga aatagtccaa tatggcgagt tggcggcagg tcgccccggc cacatagtcc
     6181 agcaaatcct ggagcaacgt cttcatgcgt tccacacggg ctg
//
LOCUS       DAOQDS010000119         6124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_7757_length_6124_cov_20.930631, whole genome shotgun sequence.
ACCESSION   DAOQDS010000119 DAOQDS010000000
VERSION     DAOQDS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7757_length_6124_cov_20.930631"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PK589_17910"
     CDS             <1..359
                     /locus_tag="PK589_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ21543.1"
                     /translation="NLAFYAAFESLDMLQMDKVWAHLEYVTCIHPGWTLRSDWPAVRD
                     SWVLIFNNTFSMKFELSDVQVQVAGDLGWVICTEHLTSRQDDQPVETRVLATNLFERI
                     GDEWLMIHHHGSPVMG"
     gene            541..870
                     /locus_tag="PK589_17915"
     CDS             541..870
                     /locus_tag="PK589_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21544.1"
                     /translation="MAQITTLSDESCRQTVRGQLESILVEEGERPEMATRLAVNTTVV
                     LATGSLGPRPFGVSSPSGTDYSFFVQRKGESCLLRLYGRQKGFTRYTNNLTYISTRPL
                     EGCACAE"
     gene            1264..1509
                     /locus_tag="PK589_17920"
     CDS             1264..1509
                     /locus_tag="PK589_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21545.1"
                     /translation="MMVKGFLLKDREEQEYLLAIQPATHGMFERLIVRQKIDGCWVEL
                     NGNAQGTWQPRTQAITAVLGYLQRLWDTSKPVAAREN"
     gene            1566..1904
                     /locus_tag="PK589_17925"
     CDS             1566..1904
                     /locus_tag="PK589_17925"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNJ21546.1"
                     /translation="MQAEKQDRGGDMMSGSSMKSREMSDDPEAAALKRKPEDLTPREL
                     EILRLIWDGHTNRSIAELLNISMKTADTHRANMMKKLRASNTAQLLKAAIEGKLLHIH
                     EGARRSDGAK"
     gene            complement(1988..2224)
                     /locus_tag="PK589_17930"
     CDS             complement(1988..2224)
                     /locus_tag="PK589_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21547.1"
                     /translation="MSGQSCLIDGCQARVYAPAGTRSICKDHFLGFLTWRRRRGPQMF
                     RKYAGMTMEERDVIAGEWLQTLGTKDLPHPIPGL"
     gene            2459..2713
                     /locus_tag="PK589_17935"
     CDS             2459..2713
                     /locus_tag="PK589_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21548.1"
                     /translation="MIMQVISVVGALMVLIAYGLIQAGTWRELDAGYLALNIIGSLLL
                     GIVAIEDRRVGFVLLEFAWAGIALVGVARSLRARRAARGL"
     gene            2768..3250
                     /locus_tag="PK589_17940"
     CDS             2768..3250
                     /locus_tag="PK589_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21549.1"
                     /translation="MAGLLSADDVFEQAKNAAVGATNADEKALQIDYEALQLKFRAAL
                     GDRKVARCHINKFLPEGYEDQGRFNLILLTAGNVIFDIVIGDSYFRYDVVSVSQLDKV
                     QVIDAMWDNREKRREEPFLSLRLMHAEETHLLLALDDEERKSLLGFASAVSAVRNPEK
                     "
     gene            complement(3432..>3867)
                     /locus_tag="PK589_17945"
     CDS             complement(3432..>3867)
                     /locus_tag="PK589_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNJ21550.1"
                     /translation="TPVGSHARELLRIEAGLAKAGPDLNEEIVPPEANLEGKAFSLSK
                     GCYPGQEVVARMDTYGNVRRHLVGLLIQDQTVPAHGSKLFSGDREVGWISSAVLSPQR
                     HAVLAFGFPLRDFSAPNTELTVEVAGTRHQATVHTLPFYQRP"
     assembly_gap    3868..3967
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3968..4317)
                     /locus_tag="PK589_17950"
     CDS             complement(<3968..4317)
                     /locus_tag="PK589_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNJ21551.1"
                     /translation="MNTTIVVAVTDIFFYTKVRDALRPQGYTLERIRNQDEVAEKMTS
                     TQPAALILNMNDLAVDAFKALETIQSNPALRSLPILAFANHEEVDTWRRAKELGVTKV
                     VSRNEFSARTKDLV"
     gene            complement(4333..4848)
                     /locus_tag="PK589_17955"
     CDS             complement(4333..4848)
                     /locus_tag="PK589_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ21552.1"
                     /translation="MDVQDFLIQGDGSEEDKREAWQLFQQAYELQMKGQLEEAVSLYK
                     LSLATHQTAEAYTFLGWTYSFMGKLDEAIEECHKAIACDPHFGNPYNDIGAYLIEKGD
                     LDEAIPWFQKAMQATRYESPAFPHLNLGRVYERQGKWSEAIDCYKQALALNPDYALAK
                     KALGRLISSLN"
     gene            complement(4987..5787)
                     /locus_tag="PK589_17960"
     CDS             complement(4987..5787)
                     /locus_tag="PK589_17960"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HNJ21553.1"
                     /translation="MGKPKPAPLRERDITRQIAREYYNEFDQLIESDVIIVGAGPSGL
                     ICAHDLARMGVKTLVVEQSLALGGGFWSGGYLMNKATICAPAHKILKEVGVPCKQIKE
                     CPGMYMVDPPHATGALIAAAYDAGAKIMNLTRVVDLILRREGVLEGVVVNSTTAEMAG
                     HDIIHVDPIALESKIVVDATGHDAVVVNLLHKRGLYQQVPGNGAMWVSRSEEEVMDRT
                     GEVSPNCFVIGLAVAAVFGTPRMGPAFGSMLLSGRYGAELIREKLKKR"
     gene            complement(5927..>6124)
                     /locus_tag="PK589_17965"
     CDS             complement(5927..>6124)
                     /locus_tag="PK589_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNJ21554.1"
                     /translation="ATLPDNAAKVAHFCSMCGPHFCSMKITQDVRDYAAQKQLEEQQA
                     IQIGMKEKSEEFKKAGSEIYL"
BASE COUNT         1225 a         1693 c         1755 g         1351 t
ORIGIN      
        1 ccaatctcgc cttttatgcc gcctttgaaa gtctggatat gctccagatg gacaaggtgt
       61 gggcacatct cgaatacgtc acctgcattc accccggctg gacccttcgg agcgactggc
      121 ctgccgtacg cgattcctgg gtgttgatct tcaacaatac cttctccatg aaattcgaac
      181 tatccgacgt gcaggtacag gtggcgggcg atctgggatg ggtgatctgc accgaacacc
      241 tcacgagcag gcaggacgac cagccggtgg aaacgcgcgt attagccacc aatctctttg
      301 aacggatcgg tgatgagtgg ttgatgatcc atcaccacgg cagccctgtc atggggtagg
      361 gggcaatgag tgcggaggcc ggatcctgtc tactggccag gcacctctgc ccgcacgggg
      421 gctacagcgg tggatgccga tgctcggcgt gtgaatggta aacgttcctg catgacaccc
      481 ttatctctcg ccgtcagatg gacaggcttc tgcgcccttc tgttgtccgg atgtaccacg
      541 gtggctcaga ttacaaccct gtcggatgag tcctgtcggc aaactgtgcg cggtcaactg
      601 gaatccattc tcgttgaaga gggagaacga cccgagatgg caactcgtct cgcggtcaac
      661 accaccgtgg tcttggcaac cggctctcta gggcctcgcc cgttcggagt ctcgtcgccc
      721 tctggcaccg attacagttt ctttgtccag cgtaagggtg aaagctgcct gctgcgtctg
      781 tatggacgtc aaaagggatt cacacgctac accaacaatc tcacctatat ttcgactcgt
      841 cctctggaag gttgtgcctg cgccgaatga ccggggctct gactgtggtc ttcttcctct
      901 cgtccatctc ctgcttctga actgaatcct tgacccggtg tgtcatgggg gctggcgctt
      961 ccggcgagcg gcagcgcggt gtcctattct gtctcgtcac ctgtctcatc ccgccaatgt
     1021 ggcacagctc tgtgaatcat gacacagcca gtgatgaccc tcctttcaaa agataatttc
     1081 gcaagtcatt gtttaatcag caatggcagc cgcattgtgg gcatggcacg ggccttgatg
     1141 tagtgctcgg tagatgtcgt aaggcgaaca ccttggggag ttcaagggag gagggaagca
     1201 atggaagtga aagagcgtcc acgggtgagc ccattgtgca ggaatcacaa ggaggaacga
     1261 tccatgatgg tgaaagggtt tttgctgaaa gatcgggaag agcaggaata cttgttggcc
     1321 attcaaccgg cgacgcatgg gatgttcgag cggctcattg tacgccagaa gattgatgga
     1381 tgctgggtcg aactcaatgg caatgcgcag gggacctggc agccgcgcac gcaggccatc
     1441 acggccgtgt tggggtacct tcaacgcctg tgggatacgt cgaaaccggt cgcagctcgc
     1501 gagaactaac ccactgatag ctcgtcacca tcagtgccgg tgagagacat tgaaggagga
     1561 ggaccatgca ggccgaaaag caagacagag gtggggacat gatgagcgga tcgtccatga
     1621 agagccgcga gatgtctgac gatcctgaag cagctgcctt gaagcgcaag cccgaagatc
     1681 tgacgcctcg cgagttggaa attctccgtt tgatctggga tggtcacacc aatcgcagca
     1741 ttgcggagct gctgaacatc agcatgaaga ccgccgatac ccatcgggcc aatatgatga
     1801 agaaactgcg ggcgtctaat acggctcagt tgctgaaggc ggcaatagaa gggaaattac
     1861 tgcacatcca cgagggggcc cggcgttccg acggcgcgaa gtgattactc atgcctatcc
     1921 gcaagcgtcg ccggcttcgc ctggtgaggc gacgcgggaa ggcacttcga gagtcagtgc
     1981 aacctgttca caatccaggg atggggtggg gaagatcttt ggtgccgagt gtctgaagcc
     2041 actcacctgc gatgacatca cgttcttcca tcgtcatgcc ggcatacttg cggaacatct
     2101 gggggccacg tctccgtcgc caggtcagaa atcccaggaa gtgatccttg cagatagaac
     2161 gggtgcctgc cggcgcatac actctcgcct gacaaccatc gattaagcaa ctctgtccgc
     2221 tcatctgtgc ctcccaatca ttctcgcgaa gtatgccgga gatgcccccg caaatgcaat
     2281 gcagcgtccg gcgttcgaga aggctcttgc caggcggcgg gcgagatggc gcaaaggctc
     2341 gatgccgtac ccgatgcctt gccccatcag agggaatttc gcttgcaatc ctgccgagtc
     2401 gccaggtacc gtaccccgtt ctccagtgag atgcgggctc gagtgcgcga gtatacccat
     2461 gatcatgcag gtcatatcgg tggtgggagc cctgatggtg ctcatcgcct atggcctcat
     2521 tcaagccggt acctggcgcg aattggacgc aggctatttg gccttgaaca ttatcggctc
     2581 actgctcctg gggatcgtag caattgaaga tcgacgagtg gggttcgtcc tgctggaatt
     2641 cgcgtgggcg ggaattgcgc tggtcggagt ggcccggtcg ctacgagcca ggcgcgccgc
     2701 acgcggcctc tgatcgtaga tgacatcata cacatcagac agtcttgtaa cgaaggagta
     2761 aggcgttatg gcagggttgt tgtcggcgga tgacgtattt gaacaggcga agaatgcggc
     2821 ggtaggagcg accaatgcgg acgaaaaggc gctccagatc gattatgaag ccttgcagct
     2881 caagttccgc gccgcccttg gtgatcgcaa agtggcgcga tgccatatca acaagttttt
     2941 gccggaaggt tatgaagatc agggccggtt caatctcatc ctgttgacgg cgggcaatgt
     3001 gatttttgac atcgtgatcg gcgattccta tttccgctac gatgtcgtgt ccgtcagcca
     3061 gttggacaag gtgcaggtca tcgacgccat gtgggacaac cgggagaagc gccgtgagga
     3121 gccctttttg agcctccggc tgatgcatgc cgaagaaacc catctgttgt tggcgctgga
     3181 cgatgaagag cggaagagcc tgcttggatt cgcgtcagcc gtgtcggccg tgcgcaatcc
     3241 cgagaagtaa atcaccgggg ccatctcgca aaacggggac cagcggtctt agtgtatgag
     3301 agtcctgcag gatggtcaaa atggccgtcc tgcaaggccg cagcaagtga agtgccgagg
     3361 cgtcccctcg gggacgttga ggctctgagc gatacgggaa cgccgcgggc gcgtgttttc
     3421 aacatcctgc ttcagggccg ctggtaaaac ggaagggtat ggacggtggc ctgatggcgc
     3481 gtccctgcca cttccaccgt cagctcggtg ttgggcgctg agaagtcgcg tagcggaaag
     3541 ccaaatgcca ggacggcatg gcgttgtggc gacaagacgg cgctgctgat ccatcctact
     3601 tcccgatctc cgctgaatag ctttgatcca tgcgccggta ccgtctgatc ctggatcagt
     3661 aagccgacta gatgccgccg aacattgcca tatgtgtcca tgcgcgccac gacttcctgc
     3721 cccgggtagc aaccttttga gaggctgaag gcctttcctt ccaaattggc ttccggcggg
     3781 acaatttctt cattgagatc aggcccggcc ttggccagac ccgcttcgat ccgcaacagt
     3841 tcgcgtgcgt gggagccgac gggtgtgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnntca acgagatctt tggtgcgcgc agaaaattcg ttccgggaca cgactttggt
     4021 cacgccgagc tccttcgcgc gtcgccacgt gtcgacttct tcatgattgg cgaaggccag
     4081 gatgggcagt gagcgcaggg caggattgct ctgaatggtt tccaacgcct tgaaggcatc
     4141 gactgccagg tcgttcatgt tcaggatgag cgcggccggc tgtgtcgagg tcattttttc
     4201 agccacctca tcctgattcc ggattcgctc caaggtatac ccctgcgggc gcaacgcgtc
     4261 tcgcaccttc gtgtaaaaga aaatgtcggt cacggccact acgatcgtgg tgttcatcac
     4321 atcctgcttg ggctagttca gggagctaat gaggcggccc aaggctttct tagccaaggc
     4381 ataatccggg ttgagagcca gggcttgctt gtaacagtcg atggcttcgc tccacttgcc
     4441 ttggcgttcg tacacgcggc ccaggttcaa gtgagggaat gccgggcttt cgtagcgcgt
     4501 ggcctgcatg gccttttgaa accagggaat cgcctcgtcc aggtccccct tttcaatcaa
     4561 ataggcgccg atgtcgttgt aaggattgcc gaagtgtgga tcgcatgcga tggccttatg
     4621 gcattcctca atcgcctcgt ccagtttgcc catgaagcta taagtccagc cgagaaaggt
     4681 ataggcttcg gccgtctgat gcgtggcgag cgagagtttg tacagactga ccgcttcctc
     4741 cagctgcccc ttcatctgta gttcgtaggc ttgctggaac agttgccagg cttcgcgctt
     4801 gtcttcctcg ctgccatcgc cttgtatgag aaagtcttgt acatccatat gcggcttctg
     4861 aaacaggttt ccggctgcgt tctcgggcgc tcactcaact cgacgtacca acacagtacg
     4921 tctcctcgat ttcgcttcct gcggccttgc tggaaacccg tttgagaagc cgcaggacag
     4981 tatgaactaa cgcttcttca gcttctctcg gattaactcg gcgccgtacc ggcctgaaag
     5041 taacatcgac ccaaatgccg ggcccatgcg tggcgtgccg aacactgccg ccaccgccag
     5101 tccgatcaca aagcaattgg gagaaacttc tccggttcga tccatgacct cttcttccga
     5161 tcgcgagacc cacatggcgc cattgccggg aacctgttga tagagccccc gcttgtgcag
     5221 cagattgacc accaccgcgt catgtccggt ggcgtcgacg acaatcttac tctctaacgc
     5281 gattggatca acatggataa tatcgtgtcc ggccatctcg gcagtggtgc tattcaccac
     5341 cactccttcg agcacccctt cgcggcgcag aatcaggtcc acgacccgcg tcaggttcat
     5401 gatcttcgcg ccggcgtcat aggcggccgc gatcaacgcg ccagtcgcat gcggaggatc
     5461 gaccatgtac atgccggggc attctttgat ctgcttgcag ggtacaccca cctctttgag
     5521 aatcttgtgc gccggagcgc agatggtagc tttgttcatc aagtagccgc cggaccagaa
     5581 ccctccgccc aaggccaggc tctgttcgac gaccagggtt ttgaccccca tcctggccag
     5641 atcgtgggcg cagatcaagc cagaagggcc ggcaccgacg atgatgacgt cgctctcgat
     5701 cagttgatcg aactcgttgt aatattcccg cgcaatctga cgggtgatgt cgcgctcgcg
     5761 tagtggtgcc ggttttggct tccccatgct acttttcctc tcagcggctg ctgaacatga
     5821 ctcccggctt cgttctcggc tccatcaaat cctcaacgta cccctgaggg tacgcctccg
     5881 gtttgagttc gcctgcggcc ttgccggata gccatgttga tcagccttac aggtaaattt
     5941 cggaaccggc ctttttgaat tcttctgatt tttccttcat gccgatctgg atggcctgct
     6001 gctcctccag ttgtttctgc gccgcatagt cgcgaacatc ctgcgtgatt ttcatggagc
     6061 agaaatgcgg cccgcacatg ctgcagaaat gggcgacctt cgccgcattg tccggcagcg
     6121 tggc
//
LOCUS       DAOQDS010000120         6103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_7800_length_6103_cov_20.350860, whole genome shotgun sequence.
ACCESSION   DAOQDS010000120 DAOQDS010000000
VERSION     DAOQDS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7800_length_6103_cov_20.350860"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PK589_17970"
     CDS             complement(<1..830)
                     /locus_tag="PK589_17970"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ21555.1"
                     /translation="MTTAHGSHVSDYMHRQLEVVPQDTSVVTVATRMRTRSIGSVLIE
                     SFDRPHNDCRIAGIVTETDLVSKVLAAGRVPSRTSMADIMSSPLITIAPDRPMVDASH
                     LMQGKNVRHLVVTEGTDVLGVISVRDLVRHFVDADGGPVQALTNVYRPLSVLMQKAIE
                     TIGSEETAMAAAQRMADKHIGALFVIEADELVGIITEGDLVRKLLAYQLDPEAMRVGA
                     LMNSPLLDIDINRTIRDASERMAAKRIRHLAVTEHEKIVGVLSIRDLVKMVAIRDRPD
                     "
     gene            complement(931..3711)
                     /locus_tag="PK589_17975"
     CDS             complement(931..3711)
                     /locus_tag="PK589_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNJ21556.1"
                     /translation="MPELTALEICRLAPSQVFRALATSPQGLSADDVRRRTLKHGPNS
                     LQALRRTPLIGRFARQFTHFLALLLWVAAGLAFLADAFHAGEGMATLGRAILGVILIN
                     AIFAFFQEYRAERAVQALRGMLPAKAWVIRDGQQQQIARSELVPGDVLVLEEGEQVPA
                     DARLTEVSGMRMDNSSLTGESKPQRRTAEPITDGHPLDIANLAFAGTTVLSGHGQAVV
                     FATGLSTEFGKIAHLTTSVQSGLSPLQQEIVKVTHVVAGLSVAMGLVFFAIGVSMGLG
                     FLSSAMFGIGIIVANVPEGLLPTVTLALAMGSQRMAARKALIKHLASVETLGCTTVIC
                     TDKTGTLTENRMRLDKLYVDDLIVESRESCLFSRNRLISAAESERWRPLFDAMIHCNN
                     AKRTRRPDGRSQATGDPTETALLEFAADHGLLHRPPLRRMGEWPFDADRKRMTTLHWS
                     EGRLLAFTKGAPESVLPRCAMQQGSSAAAELTPDGRKKILAQSQTFARQAYRVLALAM
                     REVERGVDELEADTLEQGLTFLGLVAMMDPPHREVPDAIQKCRKAGIRVVMITGDHPM
                     TAQAIASKIGLAPETPVAQPGRFVPVIEGAQVDTFSDEQLRRLLTPTAPGEPDPIFAR
                     MAPRHKMRIVSVLKEMREVVAVTGDGVNDAPALKMADIGIAMGAAGTDVAKETASMIL
                     LDDNFATIVSAIEEGRAVFLNIRKFMTYVLASNVPEVVPYLAYGLSSLPLALTVPQIL
                     AVDLGTDMVPALALAAERPHSGVMDEPPRPRTERLLNRDVLIRAYAFLGLIEAGIAMG
                     GFFLYLHSEGWTWGDPLDWNSPLYKEATTVTFAGIVAAQVANVFACRSDRFPAFQLGW
                     VSNPLLLLGVAVELTLLFIMTYSPLGHLILGTASIPAWIYGPLVLGAIGLLLADGFKK
                     SLSGRMRTRQAG"
     gene            complement(3704..4609)
                     /locus_tag="PK589_17980"
     CDS             complement(3704..4609)
                     /locus_tag="PK589_17980"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ21557.1"
                     /translation="MNMLIAVDGSEFAEWSVQMLEAIAGRPPDTVTLLHVVDSASLKS
                     SARKHAAVSKQAIAAMTKAGDQVLRRFEGLAKTALKQATTKPRTTIDTLLAHGRVADT
                     IAKVAKQKKADLLVVGSRGLSDAEHYLLGSVSRKVSALAPCPVLVVKRPLTSLSHVLV
                     AADTSKHSQGACSFLCKQFLPDSARVTVLSVVEPAVTELASKYLSRDQVEQISAPKRQ
                     AAEQLVEKLRARFLAEGYAVTPEVQFDHVTDALLRQATSRSIDLLVAGSRGLTGSERL
                     QLGSVSETLLKYAPCSVLIVRGWRA"
     gene            4936..5472
                     /locus_tag="PK589_17985"
     CDS             4936..5472
                     /locus_tag="PK589_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21558.1"
                     /translation="MVKTYLMALVGGCMVMSLALTADASAGGSTVVAHVLSDEEAVEL
                     EIPTDIGVQKERLPLYRLGAVRYFSAGIGQVERTASYPLFSLKLVFTAGGKPFVSGVA
                     VTLRQPKDGTVLTIPGEHNTGPWLFIDLPDGTYQISATLGGVTQQVNNLTVRRGHVIT
                     QHVRWAEDRSPALPAQAE"
     gene            5515..5988
                     /locus_tag="PK589_17990"
     CDS             5515..5988
                     /locus_tag="PK589_17990"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNJ21559.1"
                     /translation="MVTKRKALAKRSASAGRKPVRFEDLTVKDIVNQRVQSARLDLTG
                     MKVASLLLKAGGTVPVIDTSKQLLGVVSEHDVLLALDEGQAWGTRTVRDLMSANPYSV
                     RPETSLPTLAHVLTESDLLAVPVVNDKNRLLGMVTRRDVVRAALRPAAKRRSAAG"
BASE COUNT         1146 a         1788 c         1972 g         1197 t
ORIGIN      
        1 aaatccggcc ggtcccggat cgcaaccatc ttcaccaggt ctctgataga gaggaccccg
       61 acgatcttct catgctcggt cacagccaag tgacgaatgc gtttagccgc catccgttca
      121 ctggcatcgc ggattgtgcg gttgatgtcg atgtcgagta agggcgagtt catgagggcg
      181 cccactcgca tggcttcggg atcgagctga taggcgagca acttccgcac gaggtcacct
      241 tcggtgatga tacccacgag ttcgtccgct tcgattacaa agagcgcccc gatgtgtttg
      301 tcggccatcc gttgtgcggc ggccatggcg gtttcttcgc tgccgatggt ttcaatcgcc
      361 ttttgcatga gcacgctcag cgggcgatag acgttggtca gtgcctgtac cggcccgccg
      421 tcggcatcta cgaaatgtcg cacgagatca cggacggaaa tgacgccgag cacgtccgtc
      481 ccttcggtca cgaccagatg ccgcacgttc ttgccttgca tgagatggct ggcatcgacc
      541 atcggtcgat ccggtgcaat cgtgatgagt gggctgctca tgatgtcagc catgctggtt
      601 cgagacggaa cccggccggc ggccaagact ttggagacga gatcggtttc ggtcacgatg
      661 ccggcaatcc ggcaatcgtt gtgagggcgg tcgaaggatt cgatcaacac cgagccgatg
      721 ctgcgtgtcc gcattctcgt ggcgacggtc accaccgagg tatcctgcgg aaccacttcc
      781 aactgccggt gcatgtaatc actgacgtgc gaaccatgtg cggtcgtcat acgaacgtcc
      841 tttcgtggag agtcgtcctg tggcggcact gtgcaaccgg tgccgatggt cgttcaatca
      901 gcgggaattg ttcatggctc agtcccttca tcatccagcc tgccgggttc gcatgcgccc
      961 gctcagagat ttcttgaacc catcggccag cagaagtccg atggctccca agaccaacgg
     1021 cccatagatc caggcgggga tggaggctgt gccgaggatc agatgtccga gcggactgta
     1081 ggtcatgatg aacaggaggg tcagttcaac agccactccc aacaggagca atggattgct
     1141 gacccaaccg agttggaagg ccgggaagcg gtccgaccgg caggcgaaaa cgtttgccac
     1201 ctgggccgcg acgatcccgg cgaaggtgac ggttgttgct tccttgtaca ggggggagtt
     1261 ccagtccaat ggatcacccc aggtccagcc ctcgctgtgg aggtaaagga agaagccgcc
     1321 catggcgatg ccggcttcga tcaggcctag aaaagcatag gcccgtatga gtacgtcccg
     1381 gttgaggagg cgctctgtcc ttggtcgcgg gggttcatcc atcacgccgc tgtgaggccg
     1441 ttccgcagcc agtgccaagg cgggcaccat gtctgttcca agatcgaccg cgagaatttg
     1501 cggaacggtc aacgccagcg gaagcgacga gagtccgtac gccaggtagg gcacgacctc
     1561 gggaacgttg ctggccaaca cgtaggtcat gaatttgcgg atgttgagaa acacggcccg
     1621 gccctcttcg atggcgctga cgatggtggc aaaattgtcg tccagcaaaa tcatcgaggc
     1681 ggtctccttc gccacgtcgg ttccggctgc ccccatggca atcccgatgt ctgccatctt
     1741 cagcgccggc gcatcattca cgccgtcgcc ggtcactgcc accacttcgc gcatctcctt
     1801 cagcacggac acaatgcgca tcttatgccg gggcgccatg cgggcgaaga tggggtccgg
     1861 ttcgccggga gctgttggcg tgagaaggcg gcggagttgc tcatcgctga aggtgtccac
     1921 ctgtgcgcct tcaatcaccg gcacgaatcg ccccggctgc gcgacgggag tctccggcgc
     1981 caggccgatt ttgctcgcga tggcctgagc ggtcatcgga tgatcgccgg taatcatcac
     2041 gacgcggatc ccggcctttc ggcatttctg gatggcgtcc ggcacctcgc ggtgtggcgg
     2101 gtccatcatg gcgacgagtc cgaggaacgt caggccctgt tcgagggtat cggcttccag
     2161 ttcatcaacg ccgcgttcca cttcacgcat ggccaaggcg agcacgcggt acgcttgccg
     2221 cgcgaaggtc tggctctgtg ccagaatctt tttccgtccg tcgggggtga gttccgcggc
     2281 ggctgaggat ccctgctgca tcgcgcatcg cgggaggact gattccggcg cgcctttggt
     2341 gaacgcgagt aagcggccct cgctccagtg cagggtcgtc atccgcttgc gatcggcatc
     2401 gaagggccat tcacccatgc gccgcagcgg gggccgatga agcaacccat ggtctgcggc
     2461 aaactccagg agcgccgttt ctgtcggatc cccggtggcc tggctccgcc catccgggcg
     2521 gcgcgtccgt ttggcattgt tgcaatggat catggcgtcg aacagcggac gccaccgttc
     2581 ggattcagcg gcgctgataa ggcggtttcg cgagaacaag cagctctccc gtgattcgac
     2641 aatgagatcg tcgacataaa gtttgtcgag gcgcatacga ttttcggtca acgtgccggt
     2701 cttgtccgta cagatgacag tggtgcagcc aagtgtttcg acggaggcga ggtgtttgat
     2761 gagggctttg cgcgcggcca tccgctggct gcccatggcc agggccaggg tgacggtcgg
     2821 caggagtcct tcagggacgt tggcgacgat gatgccgatg ccgaacatcg cgctgctcaa
     2881 aaatcccaga cccatgctga cgccgatggc aaaaaacacg aggcccatgg cgacggacag
     2941 gccggccacg acgtgcgtca ccttcacgat ttcctgctgg agcgggctga gcccggattg
     3001 gacggacgtc gtcaaatgcg caatcttgcc gaattcagtg ctgaggccgg tcgcaaagac
     3061 gacggcctgg ccatggcctg aaagaacggt tgtccccgca aacgccaaat tcgcaatgtc
     3121 gagcgggtgg ccatcggtga tgggctcagc cgtccgccgt tgcggtttcg attcaccggt
     3181 gagggacgag ttgtccatcc gcattccgct gacctcggtg agccgcgcat cggcgggcac
     3241 ctgttcgcct tcctccagca cgagcacatc gccgggtacg agttcgctgc gggcgatctg
     3301 ttgctgttgg ccgtcgcgaa tcacccaggc tttggcgggc aacatgccgc gcaaggcctg
     3361 aacggcccgc tccgctcgat attcctggaa gaaggcgaag atggcgttga tgagaatcac
     3421 gccgaggatg gcccgaccca gggtggccat accctccccg gcgtggaagg cgtcggcaag
     3481 aaaggcgagg ccggcggcca cccacaacag cagcgcgagg aaatgcgtga attggcgcgc
     3541 aaaccggccg atgagcggcg tgcggcgcaa ggcctgcagg ctattcggcc catgcttgag
     3601 agttcgtcgc cgcacatcat ccgcggagag gccttgcggc gaggtggcca atgcacgaaa
     3661 cacctggctc ggcgccaggc ggcagatttc tagcgccgtc agttcaggca cgccaccctc
     3721 gcacgatgag gaccgagcaa ggcgcgtact tcaggagcgt ttccgatacg cttcccagct
     3781 gcagccgttc cgaccccgtc agtccccgcg agccggcaac gagcaggtcg atggagcgcg
     3841 aggtcgcctg ccggaggagc gcatcagtca catgatcaaa ctgcacctcc ggcgtgaccg
     3901 catacccttc ggccagaaac cgggcgcgca acttctccac cagctgctca gccgcttgtc
     3961 gcttgggcgc ggaaatttgc tctacctggt cgcgtgacag gtacttactc gccagttcgg
     4021 tgacggcggg ctcgaccacg gaaaggaccg tgacccgggc ggaatccggg aggaattgtt
     4081 tacacaggaa gctgcaggcg ccttgcgaat gttttgacgt atctgccgca acgagcacgt
     4141 gggagaggct ggtgagaggc cgtttcacga cgagcaccgg gcagggcgcc agtgcgctga
     4201 ctttgcgcga gacactgccc agcaggtaat gctctgcatc gctgaggccg cgcgagccca
     4261 ccacgagcag gtcggctttt ttctgcttgg ccaccttcgc gatcgtgtcc gccacgcggc
     4321 catgggcgag cagcgtgtcg atggtggtgc gaggtttggt ggtggcctgc ttcagtgcgg
     4381 ttttcgccag gccttcgaag cggcgcagca cctgatcgcc ggctttcgtc attgcggcga
     4441 tggcctgctt tgagacagcc gcgtgtttgc gggctgagga cttcagcgac gcgctgtcga
     4501 cgacatgcag caaggtcacg gtatccggcg ggcgtccggc gatcgcttcc agcatttgca
     4561 cgctccattc ggcgaattcc gatccgtcta ccgcaattaa catgttcatc gctgcattcc
     4621 tccatggcct gactctatcc tggaatgtag tctgcatcgg atatgcctga acgagatcaa
     4681 actctagcaa gccattccat acgctgtcga acggtgagcg gccttggcct cgtctcgggg
     4741 atcgtgccgc gatcgtggcg ctgcggcgtt ttgcagcagt gtggggtgtc agtttggggt
     4801 aattggcctc agttcaaggg tttgcggtgg gctgacggtg tgatctgatg cggcctcgta
     4861 agcgatttct gttcgaacac agggcgccgt actcaggcat caccattgcg tgacaatcga
     4921 gtaggaggaa ccgacatggt gaagacgtac ctcatggcat tggtcggcgg gtgtatggtc
     4981 atgagcctcg cgctgacggc ggatgcgagc gccggtggct cgacggtggt cgctcatgtg
     5041 ctttccgacg aggaggccgt cgaacttgag atcccgacgg acatcggtgt gcagaaagaa
     5101 cgtcttcccc tgtatcgcct gggcgcagtc cggtacttca gtgccgggat cgggcaggtc
     5161 gagcggacag ccagttatcc gctcttttcg ttgaagcttg tcttcacggc gggggggaag
     5221 cccttcgtct cgggggtagc cgtgacgctc cgtcagccca aagacggaac ggtgttgacg
     5281 attccaggtg agcacaacac aggcccgtgg ttgtttatcg acctgcctga cggcacgtac
     5341 cagatatcgg ccacgttggg cggcgtcacg cagcaggtca acaatctcac cgtgcggcga
     5401 ggtcatgtca tcacgcagca tgtccgctgg gccgaggacc ggagccctgc attgcctgcg
     5461 caagctgagt agcaatgaag gtcacaatac atgggtcgat caggaggagg aagcatggtg
     5521 acgaaacgca aggccctggc gaaacgatcg gcgagcgcag gccgcaaacc agtacggttc
     5581 gaggacctga cggtgaaaga cattgtgaac cagcgcgtcc agtcggctcg ccttgatctc
     5641 acgggcatga aggtagcgtc gttgctacta aaagcagggg gcaccgtgcc ggttatcgac
     5701 acatcgaaac aactgttggg cgtggtgagt gaacatgatg tcctgctggc ccttgacgag
     5761 gggcaggcgt ggggcactcg aacggttcgg gatctgatga gcgcgaatcc ctattcggtg
     5821 cggcctgaga ccagtttgcc cacgctggcc catgtgctga cggaaagcga cctccttgcg
     5881 gtgccggtgg taaatgacaa gaaccggctt ctgggcatgg tcacgcgccg ggatgtggta
     5941 cgagcggcat tgcggcccgc cgcgaaacga cgatctgcag cggggtagcc ggatccgctg
     6001 tccgatcgta aaggagcatg tatgcgccga atcgatctgc tgacgcaggc ttgtgatccc
     6061 gccacgctga cggtgggaca actgatgcag gatgccttgt tta
//
LOCUS       DAOQDS010000121         6041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_7920_length_6041_cov_21.971266, whole genome shotgun sequence.
ACCESSION   DAOQDS010000121 DAOQDS010000000
VERSION     DAOQDS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7920_length_6041_cov_21.971266"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PK589_17995"
     CDS             <1..983
                     /locus_tag="PK589_17995"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNJ21560.1"
                     /translation="RETQAELDSLDAQIGGTTVMAHPSVDLAKHQLIEAHLEYARQQI
                     RAPISGYVAKRKAQVGDRVQPGASLMTIVPLDHLWVEANLRETELQHVRPGQPALVSV
                     SLYGSKQTFHGTVEGLVPGSGSAFALLPPDNSTGNFIHIVERVPVRIALPADELREHP
                     IRPGLSTMTSINITESGQSVWTSLATPSTAEYETDVYADELPTAESMAKEVMATNLVV
                     SENGDGIDSLLEDEGAIERVNPRRDVDRLPNEGKAAPKLDRLRERDDRRPSSFVPRRS
                     PDLGATTFPLTPSIGPDSRSLGPEAGRNPSRLKPGFGLDDERRAFGSGRR"
     gene            1250..2770
                     /locus_tag="PK589_18000"
     CDS             1250..2770
                     /locus_tag="PK589_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ21561.1"
                     /translation="MPNVTLNYRTLLSVTNVLNSQRNRQSLFRAITDQLTKVVRWERA
                     GITVYDLQTDAFRFYAVETNLPKVVLRSDAMIPRAQSAVGWVYDHQRVHVRPHLREER
                     LFIEDESYAQEGLGRMINLPMVVQEACLGTLNIGSIEAGYPDPDDVDFLQQVATQIGF
                     AIAHVNAYEEINRLREQLARENVYLTEELKATQDYGIVAGRSRAFEAVLTLARQAAPT
                     TATVMITGETGTGKEVLARAIHERSLRRERAFVRLNCAALPSGLIESELFGHERGAFT
                     GADHQRVGRFELADGGTLFLDEIGEMPLEAQAKLLRVLQDGMVDRVGSAKPIPVDVRV
                     IAATNANLERAIQQGRFRSDLYYRLNVFPIHMPPLRERPEDIPILARHFLRFHARRLK
                     RLCEDFDASSMERLVQYAWPGNVRELENLVERGLILCHDRWLRIDPAMINLPAASEVN
                     PRRTLVDEERRHILQSLTLLDWRIEGPGGAAEQLGLAPSTLRSRLQKLGIRRPSRC"
     gene            3230..>6041
                     /locus_tag="PK589_18005"
     CDS             3230..>6041
                     /locus_tag="PK589_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNJ21562.1"
                     /translation="MMQLSRRQFLKASAGTVAVAAVADKALALTALQPVVEVDNPLGE
                     YPDRSWERVYHDQYRYDSSFTWCCSPNDTHACRIRAFVRNGVVMRVEQNYDHQTYEDL
                     YGNRGTFAHNPRMCLKGFTFHRRVYGPYRLKGPLMRKGWKQWMDDGSPELTSDAKRKY
                     KFDSRFLDDMVRVSWDTAFTYVAKGLVVIGTRYSGEAGARRLREQGYAPEMIEMMKGA
                     GVRTFKHRAGMPILGMMGKHANTRFNNCVLPLLDSWIRKVNPDQAQGGRYWNNYTWHG
                     DQDPSQPWWNGTQNCDVDLSDMRFTKLNTSWGKNFVENKMPEAHWKLESIERGARLVV
                     ITPEYNPTASRADYWIPVRPETDGTLFLGASKIILDENYQDIDFIKGFTDMPLLVRTD
                     TLQYLDPFEVVKDYQLPDFSKSYSGRLQGLTQDQIRRLGGMMVWDLAKGKAVPLHREQ
                     VGVHLAQSGIDPALTGTYRVKLLNGREIDAIPIYQMYQIHLQDYDLDTVHQVNRAPKD
                     LIVRWARDCGTVKPAAIHNGEGVCHYFHMTSMGRAAALVMMLTGNIGKFGTGCHTWSG
                     NYKVGIWQAAPWSGAGASVYLGEDPWNLNLKDDVHGKEIKYRKYYYGEEPGYWNHGDN
                     ALIINTPKYGRKVFTGKTHMPSPSKVRWVVNVNILNNAKHHYDMVKNVDPNIEMLVTQ
                     DIEMTSDVNHADVAFAVNSWMEFTYPEMTATVSNPWVQIWKGGIRPLYDTRNDLDSFS
                     GVAAKLKEMTGEQRMADTYKFVYHNRVDVYVQRILDASSTFFGYSADVMLKSEKGWMV
                     MCRTYPRHPLWEETNESKPHWTRSGRLETYRVEPEAIEYGENFISHREGPECTPYLPN
                     AIMTTNPYVRPDDYGIPVTAQHHDDKTVRNIKLPWSEIKQHANPLWEKGYQFYCVTPK
                     TRHRVHSQWSVNDWVQIYESNFG"
BASE COUNT         1281 a         1678 c         1841 g         1241 t
ORIGIN      
        1 tacgggaaac acaggcggaa ctcgactcgc tcgatgcgca gatcggcggc acgacggtga
       61 tggcgcatcc gtccgtcgac ctggccaaac accagttgat tgaagcgcat ctggaatatg
      121 cacgccagca gatacgcgcc ccgatttcag gctatgtcgc caagcgaaaa gcgcaggtag
      181 gagatcgtgt tcagcccggc gcctcgctga tgaccatcgt tccgctggat catctgtggg
      241 tcgaggccaa tctgcgggaa acggaattgc agcacgtgcg gccggggcaa ccggcgctgg
      301 tgagtgtcag tctgtatgga tcgaaacaaa cgttccacgg caccgttgaa ggtctcgtgc
      361 cgggcagcgg cagcgcgttc gccttgcttc ccccggataa ctccacggga aactttattc
      421 acatcgtcga acgggtgccg gtgcgtattg ccctcccggc ggatgaactc cgtgaacatc
      481 caatcaggcc ggggctgtcg accatgacga gcattaacat taccgagtcg gggcaatcgg
      541 tgtggacgtc tctcgccacg ccatccacgg ccgagtatga gacggacgtg tatgccgatg
      601 agcttcccac agcggagtct atggcgaagg aagtgatggc gacgaatttg gtggtcagcg
      661 agaacggaga cggcattgat tcactgctcg aagacgaggg ggccatcgag cgggtgaacc
      721 ccagaagaga cgtggacagg ttgccgaacg aggggaaggc ggctcctaaa cttgatcggc
      781 ttcgagaacg ggatgatcgg cgtccgagtt cattcgtgcc gcgacgcagt cccgatctgg
      841 gagccaccac ctttcctctc acgccatcga ttggacccga ttcgaggtcg ctgggccccg
      901 aagcggggcg caatccatct cgactgaaac ccgggttcgg tctggatgac gaacggcgcg
      961 cgtttggttc cggcaggcgc tagactccct gccatgacgt cgggccattc cggttaggtg
     1021 atggaaaagc agcaggggta gcacagatta tttttcgatg aagattgcgg ccctggtctg
     1081 gaatgcgcgg gctgcgcatc ctatgatgca ccacgcgctc cgagcaacac cgatagtcaa
     1141 cgctcgcctg gcttgctgat cgttccttgc ctctcctgcc ggcgtcccgc tccaaggcgc
     1201 gctctcagcg gtcgcagaca gcccctctac cgggccggag acacacgccg tgccgaacgt
     1261 gacgcttaat tatcgcacgc tcttgtcggt caccaatgtg ctgaattctc agcgaaaccg
     1321 gcaaagcctg ttccgtgcca tcaccgatca gttgaccaaa gtagtgcggt gggaacgggc
     1381 aggaattaca gtgtacgacc tgcagacaga cgcctttcgt ttttatgcgg tggagacgaa
     1441 ccttcccaaa gtcgtgctgc gaagcgatgc gatgattccg cgcgcgcaaa gcgctgtggg
     1501 atgggtctat gaccatcaac gcgtgcatgt gcggcctcac cttcgtgaag agcggctgtt
     1561 catcgaggat gaaagttatg cgcaggaagg gctgggtcgc atgatcaacc tgccgatggt
     1621 tgtgcaggag gcctgtcttg ggacgttgaa tatcgggagc atcgaagccg ggtaccccga
     1681 tcctgacgat gtggatttcc ttcagcaggt cgcgacccag atcgggtttg ccattgctca
     1741 cgtcaacgcc tatgaggaaa tcaatcgtct gcgggaacaa ctcgcccgcg agaatgtgta
     1801 tctcaccgaa gagctcaaag cgactcagga ctacggtatc gtcgcgggac ggagccgggc
     1861 attcgaggcg gtgctgacgc tggcgcggca ggccgccccg acgaccgcga cggtcatgat
     1921 taccggggaa accgggacgg gtaaggaggt gctcgcgcga gccatccacg agagaagtct
     1981 tcggcgtgaa cgggcattcg ttcggctgaa ttgcgccgcc cttccgtccg ggttgattga
     2041 aagcgaactc ttcggccatg aacgcggcgc gtttaccggc gcggatcatc agcgggttgg
     2101 acggtttgaa ctggcggatg gcggcacctt attcctcgat gagatcggcg aaatgcctct
     2161 cgaggcgcag gcgaagctgt tgcgtgtctt gcaggacggg atggtcgatc gagtcggcag
     2221 tgcgaaaccc attccggtcg atgtccgggt gatcgcggcg accaatgcca atctggaacg
     2281 cgcgattcag caggggcgat tccgtagtga tctgtattat cgcttgaatg tgtttcccat
     2341 ccatatgccg ccgctcagag agcggccgga agacattccc attctcgccc gccacttttt
     2401 gcggtttcac gcccgtcggc tgaagcgatt gtgtgaagat tttgatgcgt cctcgatgga
     2461 gcgcctcgtg caatacgcgt ggcccggcaa tgtgcgagag cttgaaaacc tcgtggaacg
     2521 cggactgatc ctctgtcacg atcgatggtt acgtatcgac ccggccatga tcaatctgcc
     2581 cgccgcttca gaagtgaatc cccgccggac cttggtggac gaagagcgcc gccacattct
     2641 tcaatcgctc acgttgctcg attggcgaat cgaagggccc ggcggcgctg ctgaacaatt
     2701 ggggctggcg ccaagtacgc tacgcagtcg actacaaaag ctcggcattc gtcgtccctc
     2761 ccgttgctag ataccgttct gcttttcttc tcttacgcgt ctctcctcca tgggccatgt
     2821 cattctcgcg ccacgattga tcgtggtggc gctgtggtcg ctcaccgcgg cttgtcgtgg
     2881 gtgatgcctt ccgtccgccg cgttcctgaa cgtcatcccg ttgaagcgcc gatatttcaa
     2941 ggacatcact gacgtcccat caacgaggcc tgtgtggcac gcgcagtgca caagggctcg
     3001 tcgtgatttt ttctgccctg atttttttaa tcgaagagcg tagtcccgcg cgcggcggcc
     3061 tgcgcatcgt tgcaccgacg aatgaccggg cacgggtgcg acgaaatcat caccaatctg
     3121 gaaggggggt atgcatctat cgtcctgtcc cggtcaatcg tttcatcggg tgcggcgcca
     3181 tcacctgtgg cgctgatcct gtgctctgtc ttcatttgag gaggtgttca tgatgcagtt
     3241 atcccgcaga cagttcttga aagcctcggc aggaacggtc gcagtggcag ctgtggccga
     3301 caaggccttg gcgctgaccg cactgcaacc ggtcgtcgag gtcgacaatc ctttaggcga
     3361 gtatccggac cgctcctggg aacgtgtgta tcacgaccag taccggtacg actcatcctt
     3421 cacctggtgc tgttccccga atgataccca tgcctgccgc atccgggcat tcgtccggaa
     3481 tggtgtcgtg atgcgagtgg agcagaacta tgaccatcaa acctacgaag acctctatgg
     3541 caaccgcggg acgttcgcgc ataacccgcg catgtgcttg aagggcttca ctttccaccg
     3601 tcgcgtgtac ggtccctatc gcttgaaagg gccgttgatg cggaagggct ggaagcagtg
     3661 gatggacgac ggctcccccg agctgacgtc ggatgcgaag cgcaagtaca agttcgacag
     3721 ccggttcctg gacgatatgg tccgcgtctc ctgggatacg gccttcacct atgtcgccaa
     3781 gggtttggtc gtcatcggca ctcgttatag cggtgaggcc ggagcccgtc gtctccgcga
     3841 gcagggctat gcgccggaaa tgatcgagat gatgaagggg gcgggcgtgc ggacgttcaa
     3901 gcaccgcgcc ggaatgccga ttctcggcat gatggggaaa cacgccaata cccggttcaa
     3961 caattgtgtc ttgccgctct tggatagctg gattcggaag gtcaacccgg atcaggcgca
     4021 gggcggacgg tactggaata attatacgtg gcacggcgac caggatccgt cgcaaccctg
     4081 gtggaacggc acccaaaact gcgatgtcga tttgtcggat atgcgcttca ccaaactgaa
     4141 taccagttgg ggcaaaaact tcgtcgaaaa taaaatgccc gaagcgcact ggaaactgga
     4201 atccatcgag cgcggtgcgc gtctcgtggt gatcactccg gaatataacc ccactgccag
     4261 ccgagcggat tactggatcc cagttcgtcc cgagacggac ggtaccttgt ttctcggcgc
     4321 gtccaaaatc attctcgacg agaactatca ggatatcgat ttcatcaagg gctttacgga
     4381 tatgccgctg ctggtgcgga cggacacctt gcagtatctg gatcccttcg aggtggtgaa
     4441 ggactatcag ttgccggatt tcagcaaatc gtactcggga cgattgcaag gattgactca
     4501 agatcagatc cgccggctgg gcggcatgat ggtctgggac ttggcaaagg ggaaagccgt
     4561 gccgttacat cgcgaacagg ttggcgtaca tcttgcgcag agcggcatcg atccggcatt
     4621 gaccggaacg tatcgcgtca aattgctcaa tgggcgcgag atcgatgcga taccgatcta
     4681 tcagatgtat caaattcatc tgcaggacta tgacctcgat acggtgcatc aggtcaatcg
     4741 ggcgccaaaa gatttgatcg tgcgctgggc cagggactgt gggacggtca aaccggcagc
     4801 catccataac ggtgaaggcg tctgccatta tttccatatg acctcgatgg gacgggcggc
     4861 agcgttggtc atgatgctga cgggtaacat cggaaaattc ggcaccggct gccacacctg
     4921 gtccggtaac tacaaggtgg gtatctggca ggcggcgccc tggtccggcg caggcgcgag
     4981 cgtctatttg ggggaagatc cgtggaattt gaatctcaag gatgacgtac acggcaagga
     5041 gatcaagtac cgcaaatatt attacggtga ggagcccggt tattggaatc atggcgacaa
     5101 tgccctgatc atcaacacgc cgaagtacgg ccgcaaggtg ttcaccggca agacccacat
     5161 gccgagcccc agcaaggttc ggtgggtggt gaacgtcaac atcctgaaca atgcgaaaca
     5221 ccactatgac atggtgaaga acgtcgaccc gaacatcgag atgctggtca cgcaagacat
     5281 cgagatgacc tcggacgtca accatgcgga tgtggcgttt gccgtgaatt cctggatgga
     5341 gttcacgtat ccggagatga ccgcgacggt gtccaatccc tgggtgcaga tctggaaagg
     5401 cgggatccgc cccttgtatg acaccagaaa cgatctcgac agcttttcgg gtgtggccgc
     5461 caagctcaag gagatgaccg gcgaacagcg gatggcggat acgtacaagt ttgtctatca
     5521 caatcgtgtg gatgtgtacg tgcagcgtat cctcgatgcc tccagtacct tcttcggcta
     5581 tagcgcggac gtcatgctga agtcggaaaa gggctggatg gtcatgtgcc ggacgtatcc
     5641 gcggcacccg ctctgggaag aaaccaacga gtcgaaaccg cactggacgc gatccgggcg
     5701 gctggaaacc tatcgagtgg aacccgaggc gatcgagtac ggagagaact tcatctccca
     5761 ccgcgaaggc ccggagtgta ccccgtatct gccgaatgcc atcatgacga ccaatcccta
     5821 tgtcaggccg gatgactacg ggattccggt cacggcgcag caccatgacg ataagacggt
     5881 acggaacatc aagctgccct ggtctgagat caagcagcac gcgaatccgt tgtgggagaa
     5941 gggctatcag ttctactgtg tcacgcccaa gacccgccac cgggtccata gccaatggtc
     6001 ggtgaacgac tgggtgcaga tttatgagtc gaacttcggc g
//
LOCUS       DAOQDS010000122         6030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_7945_length_6030_cov_19.623766, whole genome shotgun sequence.
ACCESSION   DAOQDS010000122 DAOQDS010000000
VERSION     DAOQDS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7945_length_6030_cov_19.623766"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PK589_18010"
     CDS             complement(<1..699)
                     /locus_tag="PK589_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21563.1"
                     /translation="MSSHQSSDRPEPRKSGIHDADLDGLAISRPAPGGIPSSGGRRWR
                     WLTLAVLLAVAGLAWSTAWLRPDQRVEVVPVVRMPSGSGAGLAATGYVVAQRQASIAS
                     KGTGRLIYFGVNVGDRVKEGQLIARIEHDDMDALYRQALARLGVARAQLGAAKPELEE
                     ATLSFERVKTLLEKSFVTKSEFDMASARLRRAAAAVKSAEAAVTAAEAERQNATVQLE
                     NTSIRAPFDGVIVKK"
     gene            complement(780..2000)
                     /locus_tag="PK589_18015"
     CDS             complement(780..2000)
                     /locus_tag="PK589_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNJ21564.1"
                     /translation="MPSESIPVAARQAAQAGVRDRRARVWRTWWVQAHLYLGLTTGAL
                     LVVFGLTGSILVFFQEIDEWLNPALLTVDATADGQNSYRPIGEILAAAEQAAAPGSRI
                     TQVYGATKPERVMAVYMEEPSKAWQRIFVDPYRARVTGVRSYGPDEWVPKYLMDAIFA
                     LHYQLFVGMTGVTLAAIAAWLLILSLVTGVIVWWPVNGQWRHAFVIRRPFALFRLLFD
                     LHKTVSLYLCLAIGAVLLSGAYMNWAEPIIWITQVLSPATRGPAQSAPSAVEDGAHPI
                     SPQRAVELAAAIYPEGRLTSIAMPEDATGVYQVGRQGVPGLSAFWSERIVSVDQYSGS
                     ILDVRAPDTRRSAGETFLDWQWPLHSGQAFGIPGRLLVCASGLACPVIYATGFLMWWR
                     KRRGRARRSLVPAS"
     gene            complement(2210..2644)
                     /locus_tag="PK589_18020"
     CDS             complement(2210..2644)
                     /locus_tag="PK589_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21565.1"
                     /translation="MVVRLKLYRVFVVNELLHIMSLRGRTGRAPSLLFFVACLLVAIR
                     VAAGFVCHTCFIDFEKPTSRSFHLHAGGDRDPCHHGQAEASPLTTWACTVTQDETAFI
                     LPEIPRLPLVVSEFVPLVLLLLSYRTLFQIAAHGRGPPVPLS"
     gene            complement(2802..5150)
                     /locus_tag="PK589_18025"
     CDS             complement(2802..5150)
                     /locus_tag="PK589_18025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNJ21566.1"
                     /translation="MKSTDSCQNASRSFIPLRRGSAGIGRIVAVAGLTLLTACIGPHK
                     PVATDKTSATPVAAPAQGESTGESGGDKAAQARQSPPALPVPLPVPVPAEDLAGTGAS
                     TRDDAPVVDVKPVHIVAQAESYHVTNTVTATKTDTPIMDTPVSVKVVPQQVMRDQQVV
                     RVDQALKNISGVISGAEGDMQFNMRGFDQFNFYRDGYPFQSQWFHGEDLTNIERVEVL
                     KGPGSILYGRAEPGGIINFVTKQPLDQPYYSLQQQVGSYGWYRTNADLTGPLTANNSV
                     SYRLNIGYQTNQTFQQFGGNERLIVSPQVRWRITDHTTSTLKYEYSDISLTGKGNIPL
                     LGNRPAPIARELNLGERWNLQNDKYHLVSLITEHAFNDAWSIRHRFNFSNHQLTLTGQ
                     NVGNAAANGDVSRFFFAQNTDGRDYTRNVFNALELTGHVTTGRIKHTLLFGGDYLHND
                     IRATMAGFTPATDDVINIFNPVHTAGPPVIAPGDVTKYTWSLPWFGLYAQDQIELPYH
                     IHILAGFRYDNAKTSTSNENLGVRTPTIKDTNERISPRAGVVWRPVPEFSLYGSYTEN
                     FGASNTFTAGSVVPLPPQTAQQWEVGAKTELFGGRFIGSLAYFDLTKQNLPVFFPGET
                     RAVGEGESRGVEFDVSGEILPGWSVIGAYAYTPFAKTTKDDPALGTMGKRLNNAPVNS
                     GSLWTVYQFQEETLQGLRVGVGVQGVGERQIGFGETAQAPGYVITNLMASYHWRVGTA
                     RYTAQLNVDNLLDKSYIGQVFSYGPTYYGMPRFFMGSIRMEF"
     gene            complement(5377..5781)
                     /locus_tag="PK589_18030"
     CDS             complement(5377..5781)
                     /locus_tag="PK589_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21567.1"
                     /translation="MKPARPFMPLSGSRGRGQRHAVLLALFLIGIRLVAGFLHVSCFN
                     EMERPSARSFHLHAGGDRVPCHHGKAPTSPLSDWACSVNQDESAYILPEVPLLPLVAA
                     LFLLFLLFRVSFGSRSAIVPHGRGPPRHLLVS"
BASE COUNT         1267 a         1935 c         1718 g         1110 t
ORIGIN      
        1 tttcttcacg atgactccgt cgaacggcgc gcgaatgctc gtattctcca gctgcaccgt
       61 ggcgttttgc cgttcggctt ctgccgcagt caccgccgcc tcggccgact tgaccgccgc
      121 agccgcgcgg cgaagtctcg ctgacgccat gtcgaattcc gacttcgtga cgaaggactt
      181 ctccaggagg gtcttcaccc gctcgaaact cagcgtcgcc tcttcgagct ccggtttggc
      241 ggcgcccaat tgtgcccgcg ccacgcccaa ccgggccaag gcctgccggt acagcgcatc
      301 catatcatca tgctcgatgc gcgcgatcag ttgcccttct ttcacccgat cccccacgtt
      361 gacgccgaag tagatcagcc gccccgtgcc cttggaggca atcgacgcct gccgctgcgc
      421 gaccacatac ccggtcgccg ccaggcctgc cccgctcccg gatggcatgc gcaccaccgg
      481 aaccacttca actcgttggt ccggtcgaag ccaggccgta ctccaggcca agcccgccac
      541 agccagcagg accgccagcg tcagccaccg ccaccggcga ccaccggaag acggaatccc
      601 accaggcgcg ggacgagaaa tcgccaaccc gtctaaatcg gcatcatgaa tgccggattt
      661 gcgaggctca ggacgatcag atgattgatg actggacacg ggaacctcct ccatggaacg
      721 gcagtgagtc gtgccgctcc ttctcgtttc cttcgcactc taatctgcca caccgcgggc
      781 tacgaggccg gtacgagcga ccgacgcgct cgcccccgtc gcttgcgcca ccacatgaga
      841 aacccggtgg cgtagatgac cgggcaggcc aggccgctcg cacagaccag caagcggccg
      901 ggtatcccga acgcttgccc cgaatgcagg ggccattgcc aatcaagaaa ggtttccccg
      961 gcgctccgcc tcgtatcggg ggcccgcacg tcgagaattg agccactgta ttgatcgacg
     1021 ctcacgatcc gttccgacca aaacgcactg agccccggca ccccctgcct gccgacttga
     1081 tacactcccg tcgcatcctc cggcatggcg atggaggtca accggccctc gggatagatg
     1141 gcggccgcca actccaccgc ccgctgcgga ctgatcggat gcgctccatc ctcaacagcc
     1201 gacggagcag attgcgcagg gccgcgggtg gccggcgaca gaacctgcgt gatccagatg
     1261 atcggctcgg cccagttcat ataggctccc gacaggagca cggcgccgat cgccagacag
     1321 agatacagcg acaccgtttt gtgcagatcg aacagcaacc ggaacagggc gaacggccgt
     1381 cggatgacaa acgcgtgtcg ccattggccg ttcaccggcc accagacgat cacacctgtg
     1441 accaaggaca ggatcagcaa ccaggccgcg attgccgcca gggtcacgcc cgtcataccc
     1501 acgaacagtt ggtaatgcag tgcgaagatc gcatccatca aatacttggg cacccactcg
     1561 tcaggcccat aactgcgtac ccccgtcacc cgggcccggt agggatcgac aaaaatccgt
     1621 tgccaggctt tcgacggctc ctccatatag acagccatga cccgctcggg tttcgtcgcc
     1681 ccatagacct gcgtgatacg gctccccggc gccgccgcct gctccgccgc cgctagaatc
     1741 tcacctatcg gccggtagct attctgcccg tctgcagtcg cgtccaccgt cagcaacgca
     1801 ggattcagcc actcgtcgat ttcctggaag aacaccagga tactgcccgt caatccgaag
     1861 accaccagca gcgcccctgt ggtcagtccc aaatagagat gcgcctgcac ccaccacgtt
     1921 cgccacactc gcgcgcgacg gtcccgtact ccggcttggg cggcttgacg agcggcaact
     1981 ggaatcgact cagacggcat ggctctccca gacatgcgat tcacattcct ccgccgacgg
     2041 tctatcgagg cagcgtggcg gtagtggcga cacctccggc atgggtgccc ttgtttcacc
     2101 gacgaggcga cgacgcgcag gacggacgaa cactcattca tgacgcgggt gacgatcagc
     2161 tcgtcaccac gcgggaattg tccttattca gtgcggataa aaccgatgat caggaaagag
     2221 gaaccggagg accacggcca tgggccgcga tttgaaacag ggtgcgatac gacagcagca
     2281 acagaacgag tggaacaaat tcagaaacca cgagcgggag tcgtggaatc tcggggagga
     2341 tgaacgccgt ttcgtcctgg gtcaccgtgc aggcccaggt cgtcagcgga ctggcttccg
     2401 cctggccgtg gtggcaggga tcgcgatccc ccccggcatg gagatgaaaa ctccgggagg
     2461 tcggcttttc gaaatcgatg aagcaggtat ggcagacaaa cccggccgcc acacgaatgg
     2521 ccaccagcag acaggcgacg aaaaagagca gcgacggggc ccgccccgtt cgcccacgga
     2581 gagacatgat gtggaggagc tcgttcacca caaacactct atacagtttc agacgcacta
     2641 ccatacgaaa ctgccaccct catgtccact acctgttgct cacaggtgct acagtccaca
     2701 ccggggtttc gtggtgcaga ccgcatcccc ccggcggctc gcgacatgtc gtcgtgaacc
     2761 actcttgttt ccgctcccgt caaacttcac gtttcacgcc cctagaactc catgcggatc
     2821 gaccccatga agaaacgcgg cataccgtag taggtcggac catacgagaa gacctgaccg
     2881 atatagcttt tgtcgagcag gttatcgacg ttcagctgtg ccgtgtaccg ggccgtgccg
     2941 acacgccagt gataactcgc catcaggttg gtgatcacat atcccggcgc ctgggccgtt
     3001 tcgccgaacc caatctggcg ctcacccacc ccctgtacgc cgactcccac tcgcaatcct
     3061 tggagcgttt cctcctggaa ctgatagacg gtccatagac tgccgctatt gacgggcgcg
     3121 ttgttgaggc gcttgcccat cgtcccaagc gcgggatcat cctttgtggt tttggcaaag
     3181 ggcgtgtagg cataggcccc gatgacgctc cagcccggca gaatttcccc gctgacgtca
     3241 aactctacac cacggctctc tccctcgccg accgccctgg tttccccggg aaaaaagacc
     3301 ggcaaattct gcttggtcag gtcgaagtag gccaatgagc cgataaatcg accgccgaac
     3361 agttccgtct tggcccccac ctcccattgt tgagccgttt gcggcggcag cggcacaacc
     3421 gaaccggcgg taaacgtatt cgaagcccca aagttttccg tataactgcc gtagagcgag
     3481 aactccggta ccggacgcca tacgacgccg gctcgtggcg aaatccgctc attcgtatcc
     3541 ttgatggtcg gcgtgcgaac gcccagattc tcgttggagg tggacgtctt cgcgttgtcg
     3601 taccggaatc ccgcgagaat atggatatgg tagggcagct cgatttgatc ctgcgcgtag
     3661 agaccgaacc agggcagcga ccacgtgtat ttcgtcacgt caccaggcgc gatgaccggc
     3721 ggtcccgccg tgtgaaccgg gttgaagata ttgatcacgt cgtcggtagc cggcgtaaac
     3781 cctgccatcg tcgcgcgaat gtcgttgtgg agatagtctc ccccgaacag aagcgtgtgc
     3841 ttgatccggc cggtggtgac gtggccggtg agctcgaggg cattgaacac attgcgcgtg
     3901 tagtcccgac cgtctgtatt ctgcgcaaag aagaatcgac tcacatcccc gttcgccgcg
     3961 gcattcccca cattctgtcc ggttaacgtc aattgatggt tgctgaaatt gaaccgatgc
     4021 ctgatcgacc aggcgtcgtt gaaggcatgc tccgtgatca atgagaccag atgatattta
     4081 tcgttctgca ggttccagcg ttcgccgaga ttcaactccc gcgcaatggg ggccggacgg
     4141 ttgcccagca gaggaatatt acctttgccc gtgagcgaga tatcgctgta ctcatacttc
     4201 agagtagagg tggtgtgatc cgtgattcgc catcgcacct gcggggacac aatcagccgt
     4261 tcattgcccc cgaactgctg aaacgtctgg ttcgtttgat agccgatatt caatcggtag
     4321 gatacggagt tgtttgcggt caacggaccg gtcagatcgg cgttggtccg gtaccagcca
     4381 tacgagccga cctgctgctg cagcgaataa tagggctgat ccagcggttg cttggtgacg
     4441 aaattaatga tcccgcccgg ctcagcccgc ccgtagagaa tggagcccgg gcctttcaag
     4501 acctccaccc gctcgatatt cgtcagatct tccccgtgaa accactgact ttggaaggga
     4561 tacccgtcac ggtagaagtt aaactggtcg aagccgcgca tattgaactg catgtcgccc
     4621 tcagcgccgc tgatgacacc gctgatgttc ttcaaggctt gatcgacccg caccacctgc
     4681 tgatctcgca tcacctgctg aggcacaacc ttcaccgaca ccggtgtatc catgatcggg
     4741 gtgtcggtct tggtcgccgt cacggtgttc gtcacgtgat aggactcggc ttgagccaca
     4801 atatgcaccg gcttgacatc aacaacggga gcatcgtccc ttgtggatgc gcctgttccg
     4861 gccaagtctt cagccggaac cgggacggga agcggcacgg gcagagccgg cggagactgc
     4921 cttgcctgcg ccgccttgtc tcctccggat tccccggtcg attctccctg agccggtgcg
     4981 gcaactggcg tggcagaagt cttgtccgtc gccaccggtt tgtgcggacc gatacaggcc
     5041 gtcagcaacg taaggccggc taccgcgacg attctgccga tgcccgcgga accgcggcga
     5101 agaggtatga aagatcgaga tgcgttctga cacgagtccg ttgacttcat cgacaacact
     5161 cctccttcgg ttcccgctct acacgcacgg aaccggaact cgccgggggc agaccggtga
     5221 cgaaggctga gcggctgcac gcagggtgta cggggctggt ccatcaaaga cctgcccctt
     5281 ccggcatcgg atgatgccgg ttccctggcg tgcggcccgc cttcggtgca gcggccgtgc
     5341 gactcccaac taggtgatat ggatgagaac cctgcgttag gagacgagga ggtgacgggg
     5401 tggcccgcgg ccgtgaggaa caatcgcaga acgactcccg aacgagactc tgaaaagcag
     5461 gaacagaaga aacagcgccg ccaccagtgg cagaagcggc acctccggca ggatataggc
     5521 cgactcgtcc tgattgacgg aacaggccca gtcactcagc ggactggtcg gcgccttgcc
     5581 gtgatggcag ggcaccctgt ctcctccggc atgaaggtga aacgaccgcg ctgacggccg
     5641 ttccatctcg ttgaagcaac tgacgtgaag aaatccggcg acgagacgaa tgccgatcag
     5701 gaacaaggcc aacagcaccg catggcgctg gccgcggcct cgagatcccg acagaggcat
     5761 gaatggacgg gcaggtttca cagaacattc cggtagaacg taaggggcct caccctacgg
     5821 aaccgcacgc agaattgtct accgatgcct gctcacgctt gctacacagc tctctgaaac
     5881 actcggacag gcccggcatt gtgaccaagg ccgggcctat atcaagaacg attcggaact
     5941 ggatcagtaa taaatcgaat acccaatcga ggccgtcgtt cctcgccccg cgaaatagaa
     6001 gctattggta aacgaaatct ggttgaacgg
//
LOCUS       DAOQDS010000123         5784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_8535_length_5784_cov_20.942224, whole genome shotgun sequence.
ACCESSION   DAOQDS010000123 DAOQDS010000000
VERSION     DAOQDS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8535_length_5784_cov_20.942224"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PK589_18035"
     CDS             complement(<1..435)
                     /locus_tag="PK589_18035"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNJ21568.1"
                     /translation="MTIDPRHKSHNLLDGPGRAPARAMLKAVGFTDADLERPLIGVAN
                     TWIEVMPCNFHLRRLSERVKAGIRAAGGTPIEYNTIAVSDGISMGTEGMKASLISREV
                     IADSIELVARGHLFDGVVALSGCDKTIPGTVMALCRLNVPSLM"
     gene            complement(658..1026)
                     /locus_tag="PK589_18040"
     CDS             complement(658..1026)
                     /locus_tag="PK589_18040"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNJ21569.1"
                     /translation="MMKHLAVPALMVAALCLTTTPAWSDKGHKGKDEKCDVAELVKDA
                     KVTIDQAIKTALEAAPGTAVEAELEKKHDKTVWEVEVLGADGAMTEVHIDAATGAVID
                     KEAKHEKHEKKDKHEKKDKH"
     gene            complement(1059..1667)
                     /locus_tag="PK589_18045"
     CDS             complement(1059..1667)
                     /locus_tag="PK589_18045"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNJ21570.1"
                     /translation="MTTKLFTLATASAIACALTISPAWANPEGGYGGHGGGYQKGQHG
                     MGAAMMEMMMHGGAGHLIRHLLKHEKEIGLSAEQVTKLKDLQLNLDKNRIKMEADIQV
                     AEREVKALTDDEKSDIGAIEAKLKQSAEVQIGLRVLSVKTRRDALALLTPEQRAKEQA
                     EHEKMMQQHKGAGAPHGKNPHGGNPHGANPHGANPHGEAKPQ"
     gene            complement(1694..2341)
                     /locus_tag="PK589_18050"
     CDS             complement(1694..2341)
                     /locus_tag="PK589_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNJ21571.1"
                     /translation="MTMVEYALLAFSSLFVIVDPIAVVPAFLAMTPRDSVAQRLRTAR
                     VACLVTVGSLSGFALFGQTLLSLLGITLPAVQIAGGLILLLVSLDMLRAQRSTVQETP
                     AEKAAGTSKDDIAITPLAIPMLAGPAAISTVILLETQATTWMLRAVLLGCLVLIGLAS
                     YSILAIGARSAKWLNPIAEKIISRLMGLLLAALAVQFMVNALTGDRGLLRGLAGH"
     gene            complement(2371..3399)
                     /locus_tag="PK589_18055"
     CDS             complement(2371..3399)
                     /locus_tag="PK589_18055"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNJ21572.1"
                     /translation="MATAQRGYYDILGVQRDASADDIKKAFRRRAREIHPDLHTGAKK
                     TEMEKKFKELNEAHEVLSDPDKRKKYDQYGQNWEQAEAYEKARQQAGSQAGRGGAAGG
                     FSGDFGDIFETFFGGRGRGGNATGFAVDGEDLETDVELSIREVATGVSRRIDLTERVT
                     CKACGGSAIVRGRPCVVCGGTGTQAEKRTIEVRIPAGVQHDTRVRITGKGQPGVNGGR
                     PGDLYLRVHIQANGVFRQKGSDIHVTLPVWPWEAALGAEVMAPTLAEPVKVKIPAGSK
                     ADSKLRLKGKGLPTSAGEHGDLFLKLKIVMPTGLSDEERALYEQLSHGRHSDPRAEIL
                     AASRRHSS"
     gene            complement(3557..4657)
                     /gene="ugpC"
                     /locus_tag="PK589_18060"
     CDS             complement(3557..4657)
                     /gene="ugpC"
                     /locus_tag="PK589_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNJ21573.1"
                     /translation="MAQLELRSISKSFRDQQVLRDMSLLVPNGSFTILLGPSGCGKST
                     LLRIIAGLDAQTAGHVLIDGKEVDHLPPAERDIAMVFQHYALYPHLSVRENLAFALKM
                     RRVPRHVIDERIEDAAQLLEIHPLLNRKPKDLSGGQRQRVAMGRAIVRKPKLFLFDEP
                     LSNLDAQLRTSMRIELKKLQQRLQATMIYVTHDQVEAMTLGDRIVVIEGGRIRQADDP
                     QQTYTAPADPFVASFIGTPPMNLWEGRLKTHGTARLFQSGDLTVPLPAHLLPALQPAP
                     STLTLGIRPEDISLKDNAPSIGVEARVDLIEDLGADLLIHCRMGHLKVVARTARNTGL
                     TAGATTPLFFAISKLHLFIDHRRADLTRTMPT"
     gene            complement(4838..5653)
                     /locus_tag="PK589_18065"
     CDS             complement(4838..5653)
                     /locus_tag="PK589_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNJ21574.1"
                     /translation="MKRRILLAVGIFATVALSLLPFLWFVLTSFKSQTEIEAAPPAWW
                     PSGSLGFYRSALFDHHLFEYVLNSIVVAGSTTLLALLLAIPAAYALARLTIPGKQGIL
                     AVLLCVSMFPQMAIAGPVWRLLDALGGLNHRWGVVLPYVALTLPLAIWILASFFKELP
                     PELEDAARVDGCGPWGTLFRITLPLATPGIFTAAILILIYAWNEFFFALLILTQPEQQ
                     TLPVGIALFQGEFTMPWGELAAASVVATLPLIGVVLLCQRWIVSGLSAGAVKG"
BASE COUNT         1088 a         1683 c         1780 g         1233 t
ORIGIN      
        1 catgagcgac ggcacattca accggcagag cgccataacg gtgccgggaa tggttttgtc
       61 gcatcccgac aaggcgacga cgccgtcgaa gagatgtcca cgcgcaacga gttcgatgga
      121 atcggcgatc acctcgcgac tgatgagcga cgccttcatg ccctcggtgc ccatggaaat
      181 accgtcggag accgcgatgg tgttgtattc aatcggggtg ccgcctgctg cgcggatgcc
      241 agccttgacc cgctccgata agcgacgcag gtgaaagttg cagggcatca cttcgatcca
      301 ggtgttggcg acgccgatca aaggacgttc gaggtcggca tcggtaaatc cgacggcttt
      361 cagcatggcg cgggccggtg cgcggccagg gccgtcgagc aggttgtgac ttttgtgtcg
      421 agggtcgatc gtcatggaaa ctccttcagt tacgggacgg gaactgcgct tcaacgcggt
      481 gcctgcgcag atcgatcgca aactttcgca tagatggacg tggagggtca atctgcggct
      541 ctgtcggtca gggcctgcca gacggtggtt ctattgaagg ggtgaaggag cgagaggggt
      601 ggaggtcaac gatgcggcag ggatgcatcc ggggatgcaa ccctgccgca aggacgatta
      661 atgcttgtcc ttcttctcgt gcttgtcttt cttttcgtgt ttctcgtgct tggcttcctt
      721 gtcgatgacc gcacccgtcg ctgcgtcgat atgcacttcc gtcatcgcgc cgtcggctcc
      781 gagcacttcc acttcccaga cggtcttgtc gtgcttcttt tcaagttccg cttcgaccgc
      841 ggtccctggt gccgcctcca acgcagtttt gatcgcctgg tcgatggtga ctttggcatc
      901 cttgaccaac tcggcgacat cgcatttctc gtctttgcct ttgtggccct tgtcactcca
      961 cgcaggagtc gtcgtcaggc acagtgccgc gaccatcaga gccggaactg ccagatgctt
     1021 catcatcgag aacctccgat tcgatgcagg tgaacggctt attgcggttt cgcctcgcca
     1081 tgcggattgg caccgtgggg attcgcgcca tgcgggttgc cgccatgggg gttcttgccg
     1141 tgcggtgcgc ccgcgccctt gtgctgctgc atcatcttct cgtgctcggc ctgctccttg
     1201 gcacgttgtt cgggagtcag aagcgcgagg gcatctcgtc tggttttaac cgagagcacc
     1261 cggaggccga tttggacttc ggcgctctgt ttcaacttcg cttcgatagc cccgatgtcg
     1321 gacttttcgt catcggtcag cgccttcact tcccgttcgg caacctgaat gtcggcttcc
     1381 atcttgatcc ggttcttgtc gagattgagc tgcaggtcct tgagtttcgt gacttgctcg
     1441 gcgctcaatc cgatttcctt ctcatgtttc aagaggtgac ggatgaggtg gccggcgcct
     1501 ccatgcatca tcatctccat catggccgcg cccatgccat gctggccctt ctggtaaccg
     1561 ccgccgtgcc cgccgtatcc gccttcggga ttcgcccagg ctggcgagat cgtcagcgcg
     1621 caggcgatgg ccgacgcggt ggccagagta aacagtttcg tcgtcattcg gttcctcctt
     1681 cggttaagtg cgattaatgt cctgccagcc cacgtaataa gccccggtca ccggtgaggg
     1741 cattcaccat aaattgaacg gcgagcgcgg ccagcaacag acccatcagt cgcgagatga
     1801 tcttctcggc gatgggattg agccactttg cgctacgcgc gccgatcgct aatatactgt
     1861 aactcgccaa tccgatcagt acaaggcatc ccaataatac cgcccgcagc atccaggtcg
     1921 tcgcttgcgt ttccagcagg atcacggtgg aaatcgcggc cggtcccgcc agcatgggga
     1981 tggcgagcgg ggtaatggcg atgtcgtctt tgctggtccc ggcggctttt tccgccgggg
     2041 tttcctgaac cgtagaccgc tgcgcgcgca gcatgtcgag tgagaccaat aacaggatca
     2101 ggccgccggc gatttgaacc gccggcagtg tgatcccgag caggctcagc aatgtctggc
     2161 cgaacagcgc gaatccgctc aatgatccga ccgtgaccag gcaggcgacg cgggcggtgc
     2221 gcaggcgttg ggcgacggag tcccgcggcg tcatggcgag gaacgccggt actacggcaa
     2281 tcggatcgac aatgacgaag agggagctga acgcgaggag ggcatattcg accatcgtca
     2341 tagcagtggc cggcctcagt gggttgtgga tcaggacgaa tggcgtcttg atgctgcgag
     2401 gatttctgct cgcggatcgc tgtggcggcc gtggctcaat tgttcataga gcgcccgctc
     2461 ttcgtcggat aggccggtgg gcatgacgat tttcagcttc aaaaacaggt ctccatgctc
     2521 gccggcggag gtcggcaaac ccttgccctt caggcgcagc ttgctgtcgg ccttgctgcc
     2581 ggcggggatt ttcaccttca ccggttccgc cagggtgggc gccatgactt ccgcgccaag
     2641 cgccgcttcc cacggccaga ccggcagggt cacgtgaata tcggagccct tctgccggaa
     2701 gacaccgttg gcttgtatgt ggacccgcag gtagagatcc cccggtctgc ctccattgac
     2761 ccccggttgg cctttgccgg tgatgcgaac gcgggtatcg tgctgaacgc cggccggaat
     2821 ccggacttca atggtacgtt tttccgcttg ggtgccggtg ccgccgcaga cgacgcaagg
     2881 gcgtcctcgc acaatggcgc tgccaccgca ggccttgcac gtgacgcgtt cagtgaggtc
     2941 gatgcgccgg ctcacgccgg tggccacctc gcggatgctc agttccacat cggtttccag
     3001 gtcttctcca tcgacggcaa accccgtggc gtttccgccg cgcccccgtc ccccgaagaa
     3061 ggtctcaaag atatccccga agtcgccgct gaatcccccg gctgcgccgc ctcgtccggc
     3121 ttggctaccg gcttgctggc gagccttttc gtaggcctcg gcctgttccc agttctgacc
     3181 atactgatcg tacttttttc gtttgtcggg atccgacaac acctcatgcg cttcgttcaa
     3241 ttccttgaac tttttctcca tttccgtttt tttcgcgccg gtatggaggt caggatggat
     3301 ttcgcgcgct cgacggcgaa acgccttctt gatgtcgtcg gcagaggcgt cccgctgaac
     3361 gccgagaatg tcgtagtagc cgcgttgtgc tgtagccatc agtgtctgtc cgtggaactc
     3421 ttaggcggcg cacagccaaa gagtgcaatc ctttccggag cttacgaggt ctccggacgc
     3481 attgcaagcg aacttgtggt ttttctccgc cttccctgcg ctcgctatta cagagataag
     3541 gaagccgagg ccacagtcaa gtgggcatcg tcctcgtgag atcggcgcgg cggtggtcga
     3601 tgaagagatg cagtttgctg attgcgaaaa agagcggtgt cgttgcgcct gcggtaaggc
     3661 cggtgtttcg agcggtacgg gctaccacct tgagatgccc catccggcag tggatgagca
     3721 gatctgcacc gaggtcttca atcaggtcca cccgtgcttc caccccgatg gacggggcgt
     3781 tgtccttaag tgaaatgtct tccgggcgga ttccgagcgt gagcgtggag ggggctggtt
     3841 gtagagccgg caggagatgt gccggcaatg gaactgtcag gtcaccgctt tggaagagac
     3901 gcgcagtgcc gtgcgtcttg aggcgtcctt cccagaggtt catcgggggt gtgccgatga
     3961 aggaggcgac gaacggatcg gcaggagcgg tgtaggtctg ttggggatca tccgcctgac
     4021 gaatgcgtcc gccttcgatg acgacgatac ggtcgcccag ggtcatggcc tcaacctgat
     4081 cgtgggtgac atagatcatc gtcgcttgca ggcgctgctg caattttttc agctcgattc
     4141 gcatcgacgt gcgtagttgg gcatccaggt tggacagcgg ttcatcgaac aaaaataact
     4201 tgggtttgcg cacgatggcg cggcccatgg ccactcgttg ccgttgccct ccggagaggt
     4261 ctttgggttt gcgattcagt agagggtgaa tctccagaag ctgggcagcg tcctcgatac
     4321 gctcgtcgat cacgtggcgt ggcacgcggc gcatcttgag tgcaaatgcg agattctcgc
     4381 gaacggagag gtggggatac agcgcataat gttggaagac catcgcgatg tcgcgttccg
     4441 ctggcggcag gtgatcgacc tccttgccgt cgatgagcac gtgccctgct gtctgggcgt
     4501 caagcccggc gatgattcgc aggagcgtcg atttgccgca gcccgacggg ccgagcagaa
     4561 tcgtaaagct tccgttggga accaacagcg acatgtcgcg caacacctgt tggtcccgga
     4621 atgatttgga tatggaacgc aactcaagct gagccatagt gttcggtgcc gttcgttgta
     4681 cgcctcacgt ctttcgccgt catctgaaga ataggatgtt caaaaaggtc gtccagcgcg
     4741 gccgcaggcg aagaaaatac cgcaggcgta ccctctgggg tacgctgagg atgttttcaa
     4801 gccgagaaca aagctggagg cctttttcaa catcctgtca tcctttgacc gcgccggcgg
     4861 acaacccgct cacaatccat cgttggcaca acaacaccac gccgatcaac ggcagagtgg
     4921 cgactaccga cgctgcggcc aattctcccc agggcatggt gaattctccc tggaagagcg
     4981 cgatgcccac gggcaatgtc tgttgctccg gctgggtcag aatcaggagc gcaaaaaaga
     5041 attcgttcca ggcataaatc agaatcaaaa ttgcagccgt aaaaatcccc ggcgtcgcca
     5101 acggcagcgt gatccggaac agggtgcccc aaggcccgca gccatccact cgcgccgcat
     5161 cctcgagctc cggcgggagc tccttgaaga agctggccag gatccagatc gccagtggca
     5221 aggtcagcgc aacgtaaggc aacaccacgc cccaacggtg gttgagtccg cctagtgcat
     5281 cgagcagccg ccagaccggt ccggcaatgg ccatttgcgg gaacatcgat acgcagagca
     5341 gcacggccag aataccttgt ttgcctggaa tggtaagccg ggccagcgca tacgcggccg
     5401 ggatcgcgag gagaagggcc aggagcgtgg tagaaccggc caccacgatg ctgttcagta
     5461 cgtactcgaa caggtgatgg tcgaagagcg cgctgcgata gaaacccaga ctgcctgagg
     5521 gccaccaggc aggtggtgct gcttcgattt cggtttgtga cttgaacgag gtgaggacaa
     5581 accagaggaa cggcaacaga ctcagcgcga cggtagcgaa gatgccgacc gcaagcagga
     5641 ttcggcgttt catgaacgtc tgcgctccag gagattcgat cctatggcgc ggaggtacgt
     5701 caacgacagc gccaggatca tcaggaacac agccacggag atggcagacc catacccgat
     5761 gcggccttcc gtaaataacg tttg
//
LOCUS       DAOQDS010000124         5677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_8814_length_5677_cov_18.480434, whole genome shotgun sequence.
ACCESSION   DAOQDS010000124 DAOQDS010000000
VERSION     DAOQDS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8814_length_5677_cov_18.480434"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1213
                     /locus_tag="PK589_18070"
     CDS             107..1213
                     /locus_tag="PK589_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNJ21575.1"
                     /translation="MYIWELPDWPRFRWQADKLLNPLGDAHLKQGRLLGKMMQLGFEV
                     RLEAELLAVTDEAVKSAEIEGEHLDPSSVRSSVARRLGVRGAALGQEDRRIEGMVAMM
                     LDATGQLDTPLTRERLYGWQASLFPTGYSGLRKVKTGGWRDDADGPMQVLSGPVGREK
                     VHFQAPPAERVDAEMDALLSWLNAPLTIDGILHATIAHLWFATIHPFADGNGRIARAL
                     AERSLARSEQSPQRFYSLAGQIHRERPEYYASLEVTQKGDLDITQRMLWFIECVSHAI
                     DAAEAGCADVLRKAAFWQRHTGLALNARQRKVLNRLLDGFEGKLTARKWAALTKCSLP
                     TAQRDIKELMDQGVLIRNEGGSKNTSYSLTGPPE"
     gene            2130..2372
                     /locus_tag="PK589_18075"
     CDS             2130..2372
                     /locus_tag="PK589_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21576.1"
                     /translation="MLKSLMDEFVVNNFETFNSVNTLVDRAVRYVVKRYDAADGRIDK
                     DGFPLDVAQRIGSSTNVRPKPRRRSAIAPLERAASV"
     gene            2857..3165
                     /locus_tag="PK589_18080"
     CDS             2857..3165
                     /locus_tag="PK589_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21577.1"
                     /translation="MRTLPRLAAGLSALLLTGCVDLSFRITPENLSVKPMAEGKTCIY
                     TLFNFGYGTNTVEQAMANATPPIQNIRSISVTMFHFLVFESHCLKVVGESAPAGEGAR
                     "
     gene            3202..3504
                     /locus_tag="PK589_18085"
     CDS             3202..3504
                     /locus_tag="PK589_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21578.1"
                     /translation="MRTFCTLTVCLAVLLLTGCVSITNRVTPEDLSVKPMAEGKTCMY
                     TLFELGYGTNTVEQAMANATPPIQKIRSVSTSLFFLLFVNKSCLIVVGEGAPASAP"
     gene            3563..3865
                     /locus_tag="PK589_18090"
     CDS             3563..3865
                     /locus_tag="PK589_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21579.1"
                     /translation="MHRLICCTCWLTALLLSGCINLSIRLTPEDLSVKPMAEGKDCAY
                     AVFGFGYGTTTVEQAMANATPPIQNVRSVGVNEFYLLGVARLCLIVVGEGAPGTTK"
     gene            5276..>5677
                     /locus_tag="PK589_18095"
     CDS             5276..>5677
                     /locus_tag="PK589_18095"
                     /inference="COORDINATES: protein motif:HMM:NF039411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HNJ21580.1"
                     /translation="MERKRKKVVTDADQQIRNYYEHQPPSETEAFPDLSETIPTSEQL
                     MGKVGHMEQDRSGLNGADPDVSPQGIDAGTETPGGSEPTPDQDLVDEIGKAAGVTYQD
                     DEPLNFDEKIAAHDEARWELNPASSEDFEERQ"
BASE COUNT         1272 a         1563 c         1647 g         1195 t
ORIGIN      
        1 gtcggtggtt cgattccact cgccggctcc atttatcaag cacttacatt gccacaagct
       61 tcgtgatgcg aataggtgta tatttgcatc atactgtgag gcgaatgtgt atatctggga
      121 attgcctgac tggccacgct ttcgctggca agctgacaag ttactgaatc cactagggga
      181 tgcccacctg aagcaaggtc ggcttcttgg caaaatgatg caattgggct tcgaggtacg
      241 ccttgaagct gaactgctgg ctgtgacgga tgaggccgtc aagagtgcag aaatagaggg
      301 cgaacatttg gacccgtcga gtgtccgctc atcggtggca cgccgtcttg gcgttcgtgg
      361 ggccgccctc ggtcaggaag atcgacgcat tgagggcatg gttgccatga tgctcgatgc
      421 tacgggccaa ctggataccc cgctcacacg tgagcgattg tacgggtggc aggcctcgct
      481 gtttccgact gggtattcag gtttacgcaa ggtcaagacc ggcgggtggc gggatgatgc
      541 cgatggcccg atgcaagtac tctcaggtcc ggtcggacgt gagaaggttc actttcaagc
      601 gcctccggct gaacgcgtcg atgccgaaat ggacgcgttg ctctcatggc tgaatgcccc
      661 cctcaccatc gacggcatcc tccatgcgac gatcgcacac ctgtggtttg ccaccatcca
      721 tccgttcgcc gatgggaacg ggaggattgc gcgcgcactt gcggagcgga gccttgcacg
      781 ctccgaacag tcgccacagc gattctatag tctggcgggc caaattcacc gcgaacgccc
      841 cgagtattac gcctcgctcg aagtgactca gaaaggcgac ctcgacatca cgcagcggat
      901 gctctggttt attgaatgcg tgtcccatgc gatcgatgcc gccgaggcgg gatgcgcgga
      961 tgtgttgcgc aaggccgcgt tctggcaacg ccacaccggt ctcgctctga atgcacgcca
     1021 gcggaaagtg ctgaatcgat tgctcgacgg atttgagggc aagctgacgg cgagaaaatg
     1081 ggcggctctc acaaaatgtt ctctgccaac tgctcaacgt gatatcaagg agctgatgga
     1141 tcaaggggtg ctgatccgta atgagggggg cagtaagaac acaagttata gcctcacggg
     1201 acctccagaa taaggccgac gaggtggatc tcacgctgat tgagaaagtg ccagaacaac
     1261 gcaagcgtgt acgtgtgtgg aaaacgaaat ggcgtggcta tggactgacc ccctagcttg
     1321 agacagccaa cattggcatc atcggacaag agtgcctgcg ctcctgtaaa gctgaccatc
     1381 ctcccttcag ggctgcggta aatcctcgtc gaaggttgct gccgtccaac tcggtaccgg
     1441 cttcgctgca gaacacaacc cgggtttcaa ttccccgtag cccacgaccc tgctcgtatt
     1501 tatgagagag cagatccact acggtttggt tgagcggaat cgtccgccgt tccccgttct
     1561 tggatttaaa caccgtgacg gttcgccgtg tgaagtctac tcctggggaa gtgtgatttt
     1621 gagggccgaa gggaattggt gttgttgaga agaaaagccc tgaggtgacg atcctcaaaa
     1681 acgtcgctga caagcttcgt ggaatcagtg atcgggtacg aacgattgcg cacgagctcc
     1741 gggtgatcgc ctcagtcttt gcgagttcct gtgccatctc ctccagggca tgatccgcaa
     1801 gatcatcctc attctagatg gccatcctgc gcgaacagtc aagcgggttc agcggtttgt
     1861 tgccgcgcag gccggtctgc tagagctgcg cgtggtgcca ctatccgtcc gacctgaact
     1921 tcgatgagtt gacctgggtg cacgtcactg gaaaactggg ccgcggcgcg gacaaggact
     1981 gatttgaaag cccgcgtcca tataaatgcc agttgatatt tttagatgga agtcccaaaa
     2041 tggcgccgct tcacaaccaa gcaattatac tcatggtgag aagagacaaa gaaaagcgag
     2101 atgcgtagat aaaaatatct ggcacagcct tgctcaaatc gctcatggat gagtttgtgg
     2161 tgaacaattt cgagacattc aattccgtga acacgctggt ggatcgtgcg gtgcggtatg
     2221 tggtgaaacg ctatgacgcg gcagacggtc gaattgataa ggatgggttc ccgctcgacg
     2281 tagcccagag gatcggctct tctacgaacg tgcgccccaa gccgaggaga agaagcgcca
     2341 tagcccccct tgagcgcgcg gcatcggtct aggcattgaa cgcaacgcta ccaaagcgac
     2401 ctgcccagac tggtacggga atggctggca gacgacatga acgtgaagtg gttcaccgcc
     2461 gaaactttaa aagcgttctc ccgagcgtgc gagttgtgca gtactgttgg cggcacgcag
     2521 tgtcgatttt gcatgaggct gattgccgag cgtgccgagt gatacgtcgg ccatgcagga
     2581 ggcaaccgcc ccatcggtca gctggccgac tacgccggac ggggggatca gagaccaagc
     2641 gtgtcttgag cataacggct ggaggctggt cgagaaatga gacgctatgt cggcatatat
     2701 tccgagcatc agcactgatc tgtttatttt ccaattatgt agcgctcacg ttatcaccgt
     2761 cgtcgacgat ccgtgcgcct gaggcgcgtg cctgttcatt ttgtcgagcg acgaacccag
     2821 ctccatacct cacaccgtag tcttggagga catcacatgc gcacactacc caggctagcg
     2881 gccggtcttt ccgcactact cctcaccggc tgcgtagacc tctcctttcg catcacgccg
     2941 gagaatcttt cggtgaaacc gatggcggag ggcaagacct gcatatacac cctgtttaat
     3001 tttggctacg gcaccaatac cgtcgagcag gccatggcca acgccacccc gccgattcag
     3061 aacattcgca gtatcagtgt aacgatgttt catttcctag tcttcgaatc acactgcctg
     3121 aaggtcgtcg gtgagagcgc tccggccggc gagggagcac gctaattgga acatgatgtg
     3181 ttcgcaagag gaggaccccg catgcgcacg ttctgtacgc tgaccgtctg tctcgccgtg
     3241 cttctgctca ctggctgcgt cagcatcacc aatcgcgtca ccccagagga tctctcggtg
     3301 aaaccgatgg cggaaggcaa gacctgcatg tacaccctgt ttgagttggg ctacggcacc
     3361 aataccgtcg agcaggccat ggccaacgcc acgccgccga ttcaaaaaat ccgaagcgtg
     3421 agcacaagtc tcttcttctt gctgtttgtg aataagagct gcctcatcgt ggtcggcgag
     3481 ggcgcaccag cctccgcacc atagcggcga cgccaacgga gacggtctgt cgacgattac
     3541 cgagtactgg agggactata cgatgcatcg actgatctgc tgcacctgct ggctgacggc
     3601 cctactcctc tccggctgca taaatctctc aattcgcctg accccggagg atctctcggt
     3661 gaaaccgatg gcggagggca aggactgcgc gtatgccgtg tttgggtttg gctacggcac
     3721 cacgaccgtt gagcaggcca tggccaacgc caccccgccg attcagaacg tccgaagtgt
     3781 gggcgtaaac gaattctact tgctaggtgt agccaggctc tgcctgatcg tggtcggcga
     3841 aggtgccccg ggcaccacca aataagcgca ccgtgacccc gcgacgactg gggtgaaccc
     3901 cttcactgag acgggttggc attagcacac gttggagtga ttgccgtcct tcctctacga
     3961 gacttctccc ggtgttgatt cctgtgcagg cctaaaaaac ggaatgcgcg cggcaccagg
     4021 cggcggtccg gacgcgaggc cacgctcaaa atcatctggg ggccctggtg caagtgtgcc
     4081 catgttggct gtctcaaact aggggatcag tccacgcggt agtacttaat ttgtgctaat
     4141 cgatcgcaac acctctaagc cgtccactcc aatcagaacc tttcccggca aattttccgc
     4201 caactgacgg cgtaacgcgg tgccatcgcc gtcaactact gcctcagcta tcggacatct
     4261 ttctctagtt cgtctccgac cttgaccgct cgcgatacgc atgtatgcga aggattaagg
     4321 aagtgtggtt cccgcagcac cctgtcacgc cttctgaact tggcgcgtat agatattctc
     4381 agcttgagga aggcatggtt cgattgcctt aatccgtctg cgaaacttgg caccagggct
     4441 ttgtccagta gcctcctcca gagaccagcg taggatgaaa gcgagtgaag caagggtgcc
     4501 tgaccctcta gcggaggaaa caacatgcga ctccgataca cacttggttt cttgttcggc
     4561 gttctactgt tgtcggcctc ctgcgaccag ggatccaagc cggccgacaa gtccacgccg
     4621 ccctcgaatg cggcgcctac atcatccaat ttaaaagaaa cgcatccgga ccagccgacc
     4681 ggactgccgc aaagtggcga agggaagcaa gtcccgacaa agccgtgaag ccttgaaggg
     4741 gtgatcattc acgggttctt acctctagga ggacgatatg gctcctacaa aatgtgccca
     4801 tccatcctgc tcgtgcaaag tcgaaggtaa aaagtattgc agtgatcgct gtcaaactgc
     4861 tgcgaccaaa cctgaggctg ccgcttcctg cccttgtcat cactccggat gccaagcaca
     4921 tcattaaagg caggtgtttc tgatcggcgt ggaaagaagc aaatgaggga gtgctcatgc
     4981 gcaccatccg ctgtcgcgaa cgattgggcg agcaggagtg tcaccgcgag actgttcccg
     5041 aacgcgagcg ggccatagcg atacaggctg ggatgtcggg tctccgttat gagtgcaccc
     5101 agggtcaccg atttcacctg agtgcggacg ggagagtgtc gaggtgcgat tgtggtctcg
     5161 acccagtgcg cagggaatga gacggcgtga tgggggggcg tacggcagat cctcgatgtt
     5221 gcccctgggt tcatggggat gattatctcc gcacaaacag agatgtgagg cacctatgga
     5281 acgaaaacgt aagaaggtcg tcaccgacgc tgaccagcag atccgcaatt attatgagca
     5341 tcagccgccg tccgaaacgg aagcatttcc tgatctgtcc gaaacgatcc cgacgtctga
     5401 gcaattgatg ggaaaggtgg ggcatatgga acaggatcgt tccggactga acggcgcaga
     5461 tcccgatgtg tcaccgcaag gcatcgacgc cgggacggaa acgccgggag gttctgaacc
     5521 gacgcctgac caagaccttg tggacgaaat cggcaaggcc gctggggtga cgtatcaaga
     5581 tgacgagccg ctgaacttcg acgagaagat tgcggctcat gatgaagcgc gttgggaact
     5641 gaatcccgcc tcttcggaag attttgagga gcggcaa
//
LOCUS       DAOQDS010000125         5653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_8871_length_5653_cov_21.974634, whole genome shotgun sequence.
ACCESSION   DAOQDS010000125 DAOQDS010000000
VERSION     DAOQDS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8871_length_5653_cov_21.974634"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PK589_18100"
     CDS             complement(<1..595)
                     /locus_tag="PK589_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21581.1"
                     /translation="MGGVLIRPIILTVCIYLFLKFIVPVLPHSAPLPSSLIFLYLMLV
                     MSGSVLFATLSGESKDAFFQPILRFLTGESGGALSSARYLVLALFPLLVGWQAYGSTA
                     SSDAPPGENRTIHPAPPGEYTGLSNPVPKTPENIMYGKGLYASRCAPCHGNFDGKGFA
                     AAGFTPPPANFKDPTTIAMLQESYLFWRIKKGGVGLPV"
     gene            complement(626..1246)
                     /locus_tag="PK589_18105"
     CDS             complement(626..1246)
                     /locus_tag="PK589_18105"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ21582.1"
                     /translation="MSEVVKLQLIGLCVVGLGTVILLFIKGQFLRVIGFVAIVLGLFA
                     LVALAVPQMASLPPAEESINIADIKTATDMATIGQKIFFSKGQCALCHSIGPSESARC
                     PDLKGIGAKLSREFIFESLTEPQAYLYLDYRHEGVPKEYPARMPYINRNPIGLSKNEI
                     LSVIAFLQQMSGEPISVSPSELDIPRATPAPVKAADAGTVAVAQAR"
     gene            complement(1313..3151)
                     /locus_tag="PK589_18110"
     CDS             complement(1313..3151)
                     /locus_tag="PK589_18110"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNJ21583.1"
                     /translation="MADRIALLAGCAAMLGWIGLPDTAMAQDAAAQAPTAYRDIPYVG
                     SRNLVWIIAQLHLLLAGFVLGVPIFAWLCEVVGWKSGDKRYDKLAKEFTKLLTSSYAT
                     TALFGGILLFLLIGFYPKLMAYLTDIFFPSFLVYCGLFLVETATLYLYWYGWDAMSEG
                     NGKKFHIFLGFLLNVFAFFIMIVPNSWATFQASPVVIAEGTDFERAWAATWNPTWWPV
                     NIHRIIANVVLGGYICGAYAGIRYLSAKSTEERYHYDWMGYVGNFIGVFGLLPLPFAG
                     YWLMREIYQYNQQMGITLMGGFLSWLFILQAMLIGVLFLGSNYYFWMGITHRIPGSEG
                     QYRKPIMAMLIILLMCLAVWMTPHSLVASLEEAQKMGGTHHPLLGVFGVMSAKMTVSN
                     LMVLVTFMSFIMYWRAGKEDTAGWAKVAKSVMGAVLVLAGLAVIVLGVWGYFVPAIIR
                     INYFSTSQVAIVIFVMLTITPLTALLLKSAKTTTEMVWGRMPPRAGYSLVLNAVMVIL
                     LMTLMGYARSSSRVHWHVYGVMRDSSQYAFSPALGYAAALMALCTFLFCMLVAFIFWV
                     ATMGDKAKAAASAKGAPEPGHIPAMAGGSPALDGPASDASEGNRPV"
     gene            complement(3222..4223)
                     /locus_tag="PK589_18115"
     CDS             complement(3222..4223)
                     /locus_tag="PK589_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21584.1"
                     /translation="MSSFKLGLSILWPACWTALPLKMAFAMLFMAMGTIHLETKLGIT
                     FLMLLMSPVSVFAFFVISLGVGFHFGEGTGLPLLFLVSIPIDIWALGLVARTVFLERL
                     RLEPPDSLGLGLWVRFAIAGAVYLPLLWVVEGGATDLARSIVKAILDMDMLKGLPVAE
                     RIGLEFTAWGSASLVVLIALTFIGLTILGKLVEGKTAGAQPSGDSYQGLISRWDMIRV
                     PADQSLMLTAFTAAGAVLSVLFWMVLPVSTPHPHECCKPASVAPVAPFDPQKSLSKGE
                     KVLKDAEVKIAALEQKTAEDEKDKQKGGKEKAGVKDSSAKSDGKASQVTAGKPAGSK"
     gene            complement(4260..5375)
                     /locus_tag="PK589_18120"
     CDS             complement(4260..5375)
                     /locus_tag="PK589_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNJ21585.1"
                     /translation="MDTTTQPTTTQPSAIEKVFAFVDERVGLKTLQAKMLNEPVPGGS
                     RWAYVFGSVLLFIFIMQAVTGILLMFYYVPTADHAYASTQYIIHTVDYGWFLLSYHFW
                     GSTAMVVCVFAHMSQVFLWGAYKKPRELIWLVGLALFGIVMGFGFTGYLLPWDQRAYW
                     ATTVGVEIMDKTPLLGDFMARFLKGGATPGQMTLSRFFVIHVMVLPAALMGLAGLHLF
                     LFRKAGPAGPFRGSVEEIKAKTDYFFPRQIWKDIVGMVAVFLCICSLAFWEPIVLLEE
                     ATPDPGDYHPEPEWYFLFLFQLLRLKVFAGEFGQFLGAIALPGVFMALLAALPFIDRD
                     PERNIFKRPIALISWIVVMASILIFTVAAIINREFLE"
     gene            complement(5375..>5653)
                     /locus_tag="PK589_18125"
     CDS             complement(5375..>5653)
                     /locus_tag="PK589_18125"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNJ21586.1"
                     /translation="HIWTNKKDVPFVAYSGKCPHLGCGFKWRTHKTLGQVFLCPCHLS
                     IYDAAGKVLDGPAPRPLDPLPIKVTATGEITIIDMEFKAGTKAQVRIV"
BASE COUNT         1367 a         1639 c         1567 g         1080 t
ORIGIN      
        1 cgacaggaag gccgacgcct cccttcttga tccgccagaa cagatagctt tcctgtagca
       61 tggcgatcgt cgtcggatcc ttaaagttcg ccggcggggg cgtaaatccc gctgccgcga
      121 agcccttacc atcaaagttg ccatggcagg gcgcacagcg tgaggcgtag agacccttgc
      181 catacatgat attctccggc gtcttgggta ccggattaga cagtccggta tattcgccgg
      241 gaggagcggg gtgaatcgta cgattttcac ccgggggcgc gtcactcgac gccgtgctcc
      301 cgtaggcctg ccatccgacg agcaacggga acaatgccaa cacgagatat cgggcgctac
      361 tgagcgctcc gccactttcc ccagtcagga agcggaggat gggttgaaag aaggcatctt
      421 tcgattcacc gctcagggtg gcaaataaca cggagcctga catcaccagc atcaggtaca
      481 gaaaaatcaa gctcgaaggc agcggcgcgc tgtgcggcaa caccgggaca atgaatttca
      541 agaacaaata gatacagacc gtcagtatga ttggtctgat gagtacgcct cccatacctt
      601 cctcctctac ttcaccgcca ataacttatc gtgcttgggc cactgccacg gtgccggcat
      661 ctgcggcctt caccggtgcg ggggtggcac gtgggatgtc aagttccgaa ggactcacgc
      721 tgatcggctc gccactcatt tgctgcagga acgcaatgac cgacaggatc tcgttcttcg
      781 agagaccgat gggatttcta ttgatgtacg gcatacgcgc cggatattcc ttcgggacac
      841 cttcatgtcg gtagtccagg tacagatacg cctgcggttc cgtcagactt tcaaagatga
      901 attcccgcga caatttggcg ccaatgcctt ttaggtccgg gcagcgggct gactcgctcg
      961 gcccgatcga atgacagagg gcgcactgac ctttgctgaa aaatattttt tgaccgatcg
     1021 tcgccatgtc cgttgccgtt ttaatgtcgg cgatattgat gctctcctcc gccgggggta
     1081 acgacgccat ctgcggcacc gccaacgcca ccagggcaaa taagcccaac acgatcgcga
     1141 caaatccgat gactcggaga aattgcccct tgatgaacaa aaggatgacc gttccaagtc
     1201 cgacaacgca cagaccgatt agctgcagtt tgactacttc gctcataagt cctcaactaa
     1261 ggataatgat cacgacccgc ccctggttac cagaagaaga tcatcttccc gatcagaccg
     1321 gacggtttcc ctcacttgca tcgctcgccg gaccatccag tgcgggcgat ccgcctgcca
     1381 tggcaggaat gtgacctggt tccggagcac ccttcgcgct cgccgcagct ttcgccttgt
     1441 cacccatcgt ggcgacccag aaaatgaagg cgaccagcat gcagaacagg aaggtacaga
     1501 gggccatcag ggcggccgcg tacccgagcg cgggcgagaa ggcatattga gaagagtcac
     1561 gcatcacacc gtacacatgc caatggacac gcgacgagga gcgggcataa cccatcaagg
     1621 tcatcaggag gatgaccatg acggcattca agacgagtga gtagccggcg cgaggcggca
     1681 tacggcccca caccatctca gtggtggtct tagcgctctt gagcaataac gccgtcaacg
     1741 gagtgatggt caacatgacg aagatgacga tcgcgacctg ggacgtcgag aagtaattga
     1801 tacgaataat ggccggtacg aagtagcccc acacaccgag cacgataacc gcgagtccgg
     1861 caaggaccag cacagcgccc atcacagact tagccacttt ggcccatccc gcagtgtctt
     1921 ctttgcctgc gcgccagtac atgatgaagc tcataaacgt cacgagcacc atcaggttcg
     1981 ataccgtcat tttggccgac atgacgccga acacgccgag gagcgggtgg tgtgtgccgc
     2041 ccatcttttg cgcttcttcc aaacttgcga cgagggagtg gggtgtcatc cacaccgcaa
     2101 gacacatcag gaggatgatc agcatcgcca taatcggttt gcgatattgg ccctccgagc
     2161 ctggtattcg atgcgtaata cccatccaga aatagtagtt ggagccgagg aagagaacac
     2221 cgatcaacat ggcttgcagg atgaacaacc aggagaggaa tccgcccatg agcgtgatgc
     2281 ccatctgttg attgtactgg tagatttcac gcatcaacca atagccggcg aagggcaggg
     2341 gcaacaagcc gaagacgccg atgaagttgc cgacgtagcc catccaatcg tagtggtacc
     2401 gttcttccgt gctcttggcc gacaggtacc gaatacctgc gtaggctccg caaatgtagc
     2461 ctcccagcac cacgttggcg atgatccggt gaatattcac cggccaccag gttggattcc
     2521 aggtcgccgc ccaggcccgc tcgaagtcgg tgccttcggc aatgacgacc gggcttgcct
     2581 ggaaggtcgc ccaggagttc ggaacgatca tgatgaaaaa ggcgaacaca ttgagcagga
     2641 agcccaaaaa gatatggaac ttcttcccat ttccctcgga catcgcatcc cagccgtacc
     2701 aatagaggta gagggtcgcg gtttccacga ggaagaggcc gcagtagacg aggaaggatg
     2761 ggaagaaaat gtcggtcaaa taggccatga gcttcgggta gaagccgatc aacaggaaca
     2821 gcaggatgcc accgaacaac gcggtagtgg cgtaggatga cgtcaggagc ttggtgaact
     2881 ccttggcgag cttatcgtag cgcttgtcgc cggacttcca accgaccact tcgcacaacc
     2941 aggcaaaaat gggtacgcca agcacgaatc cggcgagaag gaggtgtaac tgggcgatga
     3001 tccagaccag attgcggctt ccgacatagg ggatgtcacg ataggcggtg ggcgcttgag
     3061 cagccgcgtc ctgagccata gccgtgtccg gcaagccgat ccagccgagc atcgccgcgc
     3121 atccggccag caacgcgata cggtctgcca tgtggcgcac ctgattatcc atagtccgcc
     3181 cttggttcct cattgttctc acctcgtcgt tcgccatgat gtcatttgct tcccgccggt
     3241 ttcccggctg ttacctgtga agccttgccg tcgctcttgg cggagctgtc cttgacaccg
     3301 gctttttcct tgccgccctt ttgcttatct ttctcgtctt cagccgtctt ctgttctaac
     3361 gcggcgatct tcacctccgc gtccttcaac accttttccc ccttggagag agatttttgg
     3421 gggtcgaatg gtgcaacagg agcaacgctg gctggcttac agcattcgtg aggatgtgga
     3481 gtggagactg gaagcaccat ccagaacaac acgctcagga ccgccccagc tgccgtaaag
     3541 gcggtgagca tcagactctg atccgccggc acgcgaatca tatcccaccg agagatcagc
     3601 ccctgataac tgtcccctga cggctgggct ccggctgtct ttccttcgac caacttgccg
     3661 agaatggtca gtccgataaa ggtcagggca atcaacacga ccagagaggc actgccccac
     3721 gcggtaaact ccaggccgat gcgttcagcc actggaagtc ccttcagcat gtccatgtcg
     3781 agaatggcct tcacgatcga acgggcgaga tccgtggctc ctccctccac cacccagagc
     3841 aggggcagat atacggcccc ggcaatagcg aaacgaaccc ataaccccaa cccgagtgaa
     3901 tccggtggtt caagcctgag gcgctccaga aatacggtac gcgccacgag gcccagcgcc
     3961 cagatatcaa tggggatgga taccaggaag agtaagggaa gcccggtgcc ttcaccaaag
     4021 tgaaatccga cgcccaagga aatgacgaaa aatgcaaaca cactcaccgg gctcatgagc
     4081 agcatcagga aagtaatccc gagcttggtc tccagatgga tggtgcccat agccataaac
     4141 aacatggcga atgccatctt gagcggcagg gcggtccaac aggcaggcca gagaatgctc
     4201 agccccaatt taaatgacga catcgtctct cgaccagtca tgtgacgaag accctccggc
     4261 tattccaaaa attcgcgatt gatgatcgcc gccacggtga aaatgaggat actcgccatg
     4321 actacaatcc agctgatcaa ggcaatcggc cgtttgaaaa tatttcgttc tgggtcgcga
     4381 tcaataaagg gcaaggccgc cagcaaggcc atgaagaccc cgggcaacgc aatcgcgccc
     4441 aggaactgtc cgaattctcc cgcgaagact ttcagacgta acagctggaa cagaaacagg
     4501 aagtaccact caggttccgg atggtaatca ccgggatccg gggttgcctc ctcgagcagc
     4561 acgatcggtt cccagaaggc caagctgcaa atgcagagga acacggccac cattccgaca
     4621 atgtctttcc agatttgtcg cgggaagaaa tagtcggtct tggctttgat ctcctcgacg
     4681 ctgccacgga acggaccggc ggggccggct ttccggaaca ggaagaggtg caaaccggcc
     4741 agacccatga gcgcagccgg caagaccatg acatggatga cgaagaagcg gctcagcgtc
     4801 atctgtcccg gcgtcgcccc acccttcagg aaccgggcca tgaagtcgcc cagcaacggc
     4861 gtcttgtcca tgatttcgac gccgacggtc gtggcccaat aggcccgctg gtcccaggga
     4921 agcagatatc cggtgaagcc gaatcccatc acgatgccga acagggcgag accgaccaac
     4981 cagatgagtt cgcgaggctt tttgtatgcg ccccaaagaa agacctggga catgtgcgca
     5041 aacacgcaga caaccatcgc cgtggagccc cagaagtggt aactcaggag gaaccatcca
     5101 taatccacgg tgtggatgat gtactgtgtg ctggcgtacg cgtggtccgc ggtcggcacg
     5161 taatagaaca tcaacagaat gccggttacc gcctgcatga tgaagatgaa gagcagcacc
     5221 gagccaaaaa cataggccca ccgtgagccg ccggggacag gctcgttgag cattttggct
     5281 tgcaacgtct tcaacccgac gcgttcgtcg acaaaggcga agactttttc aatggctgag
     5341 ggctgcgtgg tcgtcggctg cgtagtcgta tccattagac aatccgaacc tgggccttag
     5401 tgcccgcctt gaattccata tcaataatgg tgatctcacc cgttgcggta actttgatgg
     5461 gcagggggtc caacggtcgt ggagccgggc catccaggac cttccccgcg gcgtcgtaaa
     5521 tgctcaagtg acacggacag agaaagacct gaccgagggt tttgtgcgtc cgccacttga
     5581 atccacaacc cagatgcgga cacttgcctg aataggcgac aaacggaaca tccttcttat
     5641 tcgtccagat atg
//
LOCUS       DAOQDS010000126         5571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_9080_length_5571_cov_18.355149, whole genome shotgun sequence.
ACCESSION   DAOQDS010000126 DAOQDS010000000
VERSION     DAOQDS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9080_length_5571_cov_18.355149"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..122)
                     /locus_tag="PK589_18130"
     tRNA            complement(33..122)
                     /locus_tag="PK589_18130"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(86..88),aa:Ser,seq:tga)
     gene            complement(149..1435)
                     /gene="serS"
                     /locus_tag="PK589_18135"
     CDS             complement(149..1435)
                     /gene="serS"
                     /locus_tag="PK589_18135"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNJ21587.1"
                     /translation="MYDLRVLRDNLDELRDQLGPRGKDVAWEDLRKLTEDRRAQTIQV
                     EDLRHQLKKGSEEVARLKREKQPAEEAMAAMKALGETIKGHEDRLRLIEEQLAHIALR
                     IPNLPHASVPNGHDAADNVEVRRWGTPPQLSAPAQSHWDIGESLGILDFDRAVRMAKA
                     RFAVLTGLGARLERALINYMLDMHTTQHSYREVMPPLMVNRAAMTGTGQLPKFEEDLF
                     QMRDEELFLIPTAEVPVTNLHREEILAEETLPIRYTAYTPCFRREAGSYGKDTRGLIR
                     LHQFNKVELVAFAKAEHSYDELERLTSHAEAVLQGLGLHYRVITLCKGDMGFSSAKTY
                     DIEVWLPSQQTFREISSCSNFEAFQARRANIRCRPKAGKKDSKPEFVHTLNGSGLAVG
                     RTLVAILENYQQQDGSVAIPAVLQPYMGGLQTISRS"
     gene            1562..>2179
                     /locus_tag="PK589_18140"
     CDS             1562..>2179
                     /locus_tag="PK589_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNJ21588.1"
                     /translation="MIAVKRISNPPNRFEPLEREALEPSLRARIQLFEDNTHQILSQN
                     DSPDLPFKWSVNPYRGCFHACAYCYARPSHEYWGFGAGTDFESKIVLKRRAATLLRQS
                     FEKPSWKGELVVFSGNTDCYQPLEATLGLTRACLQVCAEYRNPVGIITKGVIPTRDLD
                     LFTQLRERAWIRVYFSIAFADDETARLVEPQAPGISARFEAMRVLS"
     assembly_gap    2180..2279
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2280..3236
                     /locus_tag="PK589_18145"
     CDS             <2280..3236
                     /locus_tag="PK589_18145"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNJ21589.1"
                     /translation="GPLVLPKPIPVNPFMRGGRALVCIVYGTDPARMLWRALELLGGI
                     DRMGVRGARVLLKPNVLNDQPPPSTTNPLVVKAMANIVRANGAAEVVVADGSGIIRLP
                     TSANLTSTGMRTAAESAGARVLALEDEPWVRLEPPNAKAIGHFYFSKPVYDADVVINM
                     PVIKTHRFAEFSCSLKNFVGAVHPRYRPSVTFWSGDWHERIAEINLAVHPVLTVADGT
                     TTMIDGGPTSGTPSRTDLLICSGDRVAVDLTALALLRSFGKSSKLQGRRIVDQRQIRR
                     AADLGLGVGSGRGIELVTEAVDPAPPAFLRLVEHIRQELLES"
     gene            complement(3240..3797)
                     /locus_tag="PK589_18150"
     CDS             complement(3240..3797)
                     /locus_tag="PK589_18150"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNJ21590.1"
                     /translation="MGVKWHGAGRRAVVVGALVAATLGLFSTSPAAEFKMGVVDPQVI
                     LEKSKAGKRALDGLREYVATRQKLLSRDEEELRNTEKQLKEQASKLTDAEKKDKETSF
                     RSKIQDYQKRAQEFNQELQGKQKELVDEYMKKIAVATQAVADKGGYQLVLDKGSEQTV
                     KIVIFNKDTIDLTEQVIKEFDRTNK"
     gene            3997..4398
                     /locus_tag="PK589_18155"
     CDS             3997..4398
                     /locus_tag="PK589_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21591.1"
                     /translation="MSSEQEQAHRLFIRALQHEAISCERAGCPGPVETLDQSQTRDRV
                     KTFDMTCERCGWHTSVTGREQLTPLWDNASLELMADEHLLHQQPVCPYDDTPVVFISM
                     PNPRRKAKYRVACFYCGRQIEMDWPPPESKR"
     gene            complement(4429..>5571)
                     /locus_tag="PK589_18160"
     CDS             complement(4429..>5571)
                     /locus_tag="PK589_18160"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNJ21592.1"
                     /translation="VSLDPEKHTLADLQEALQTAGVNGIELEEPRALQLQQSNDADAM
                     AGEGGTRVGSSAGGQENSKEKRKAIAKGRYAKAASALGDLTKSTRAGGTVSFKQAKRA
                     IQNIVELMMQDESTLLGLTNLRCHDQYTHNHSVNVSLLAMALGNRAGYPKVDLADLGL
                     SALFHDVGKCAISLDVLNKPGEFTQDEWAIMRSHPIEGVFTLVKARGINNVPARMASA
                     SFEHHMNYDFSGYPKLKVPWSQTVASRIITIADCYDAMTSSRVYRREPMSPANVLKFM
                     FGKSGQSFDPVLLKLFVNCVGIIPIGSLVRLDSGPLAVVIKPAQDKANAERPMVRVIT
                     DTEGTPIEDGPELDLAAQDDTGTYTHNILQLVDNAEYHFDTGRYFV"
BASE COUNT         1067 a         1566 c         1578 g         1260 t
ORIGIN      
        1 aggtcgcaat acgcaggttt cgcctgataa tttggcggag ggggcgagat ttgaactcgc
       61 ggataggtta ccccatctcc ggttttcaag accggcacgt tcggccactc cgtcacccct
      121 ccacacctgg cccggacact tatgaccatc aggatcgcga gatcgtctga agccctccca
      181 tatagggttg taacactgct ggaatagcca cactgccatc ctgctgctgg taattctcga
      241 gaatagccac cagcgttctg cccacggcca gacccgatcc gtttagcgta tgaacgaatt
      301 ccggtttgga gtcttttttt ccggctttgg gacggcaacg tatgtttgct cgccgtgctt
      361 gaaacgcttc gaaattgctg caggacgaga tttcacgaaa cgtctgctgc gagggcaacc
      421 atacctcaat gtcataggtc ttcgcggaag aaaatcccat gtcgcctttg cagagcgtga
      481 ttactcgata atgcaatccc agcccttgga gcaccgcttc cgcatgggac gtgaggcgct
      541 ccagttcatc gtaggagtgt tcggcctttg cgaaggcgac gagttccact ttgttgaatt
      601 ggtgaaggcg aatgaggccg cgcgtgtctt tgccgtagga gcctgcttcg cgccgaaaac
      661 acggggtgta ggccgtgtac cgaatcggca gggtctcttc cgctaatatc tcttctcggt
      721 gcagattagt gacgggaact tctgccgtag ggatcaaaaa cagttcctcg tcgcgcatct
      781 ggaacaaatc ttcttcgaat ttgggtaatt gacccgtccc ggtcatcgcc gcgcgattga
      841 ccatcagcgg gggcatgact tcgcgatagc tatgctgcgt ggtatgcatg tccagcatgt
      901 agttgatgag cgcacgctcg agacgagctc cgagcccggt caggacggca aagcgagcct
      961 tggccatgcg caccgcccgg tcgaaatcga gaatgcctaa cgactccccg atatcccagt
     1021 gcgactgcgc cggcgccgag agctgcggcg gcgtgcccca tcgacggact tcgacattat
     1081 ctgcggcgtc atgaccattc ggcacagagg catggggaag attgggaata cgcagcgcga
     1141 tatgcgccag ctgttcttcg atcaatcgaa gccggtcttc atggcctttg atggtttctc
     1201 ctaaggcttt catcgccgcc atggcctctt cagccggctg cttctctcgc ttaagtcggg
     1261 cgacttcctc ggagcccttt ttgagctggt gccgaaggtc ttcaacttga atcgtctggg
     1321 cacgacgatc ttcggtcagt tttctcaaat cttcccaggc aacgtccttc ccccgtggcc
     1381 ctagctggtc acggagttcg tccagattgt ctcgcaatac ccgaaggtcg tacatactct
     1441 ttcccctaga agcggcgtga aatttaacac ccgatgcctg cgtagtcaat gaaactggtc
     1501 gctgatcgct ggaaccgaag gcgcttgggc gtcattgaca acgcgcgggt ggggcggcac
     1561 catgattgct gtgaaacgga tttcgaatcc tcctaatcga tttgaacccc ttgagcgcga
     1621 agcgcttgaa ccatcgctgc gcgcccgtat tcagctgttc gaagacaaca cccaccagat
     1681 tcttagccag aatgacagcc cggacctccc gttcaaatgg agcgtgaatc cctaccgggg
     1741 gtgttttcat gcctgcgcct actgttacgc gcgcccatct catgagtatt ggggattcgg
     1801 agccggaact gatttcgagt cgaaaatcgt gctcaaacga cgggcggcaa ccttgctgcg
     1861 acagtcgttc gagaagccct cctggaaggg cgagttggtg gtgttttctg gcaataccga
     1921 ttgttatcaa cccctcgagg ccaccctcgg tttgacgaga gcctgtcttc aagtctgcgc
     1981 cgaataccgg aatcctgtag gcatcatcac aaaaggagtc attccgacac gtgatctgga
     2041 tctgttcaca caattgcgcg aacgggcatg gattcgcgtc tacttcagta tcgcctttgc
     2101 cgatgatgaa accgcgcgcc tggtggaacc gcaggctccc ggcattagcg cacgctttga
     2161 ggcgatgcgt gtgctctcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     2281 ggccgttggt gttgccgaag cccattcctg tcaatccatt catgcgggga gggcgcgccc
     2341 tcgtctgcat tgtgtacggc acagacccgg cgcgaatgct ttggcgtgcg cttgaacttc
     2401 ttggtggaat cgatcggatg ggcgttcgcg gcgcccgtgt cctgctcaaa cccaatgtcc
     2461 tgaacgatca gccgccgccc tcgacgacca atccgctggt ggtgaaggct atggctaata
     2521 tagtcagagc gaatggagcg gcagaagtgg tggtggccga cgggtccgga atcatccgcc
     2581 tgcccacctc tgccaattta accagcaccg gtatgcggac ggcagcggaa tcagccggcg
     2641 cgagggtgct ggcgcttgaa gatgagcctt gggtgcggct cgaaccgccc aatgccaagg
     2701 ccattggcca tttctatttc tcgaaaccgg tgtatgacgc tgatgtcgtg attaatatgc
     2761 cggtcatcaa gacgcatcgc tttgccgagt tctcctgtag tctcaaaaat ttcgtgggcg
     2821 cggtccatcc acggtaccgt ccctcggtca cattctggag cggcgattgg catgaacgca
     2881 ttgctgagat caatctcgcg gtacatcccg tactgaccgt ggccgatggg accacgacca
     2941 tgatcgatgg cggtccgaca tcaggtaccc cgtccaggac ggatcttctg atctgcagtg
     3001 gagaccgagt tgcggtggat ctgacggcgc ttgcgctgct acggtcgttc ggcaagtcgt
     3061 cgaaactgca gggcaggcgg atcgttgatc aacggcagat ccgacgcgcg gcggatttgg
     3121 gcttaggagt cggatcagga cgcgggatcg aacttgttac ggaggcggtg gacccggctc
     3181 caccagcgtt tcttcggctg gtggagcaca ttcggcagga attactggag tcctgaccgt
     3241 tacttgttgg tgcggtcgaa ttccttgatg acttgttcgg ttaaatcgat cgtgtcctta
     3301 ttgaagatca cgattttcac cgtttgctcg ctacccttat ccaacaccaa ttgatagccg
     3361 cccttgtcgg caacggcctg ggtggctacg gcgatttttt tcatgtactc gtcgacgagt
     3421 tccttctgct tgccctgcag ttcctggtta aactcctgcg cgcgcttttg ataatcctgg
     3481 atcttgctcc ggaacgacgt ttctttgtcc ttcttttcgg cgtcggtcag cttggaggcc
     3541 tgctctttga gctgcttttc cgtattgcgc aactcttcct catcccggga caacagtttc
     3601 tggcgtgttg ccacatactc ccgcagtccg tccagcgctc gcttgccggc cttactcttc
     3661 tcgagaatca cctgaggatc gacgaccccc atcttgaact ctgccgccgg actcgttgaa
     3721 aacaagccga gggtcgctgc gacaagcgcc ccgaccacga ccgcgcgccg gcctgctccg
     3781 tgccacttca cacccatctc gggactcctt tcgcgggaat aatgtcgtgc tacaaacctc
     3841 gggagaatag ctgcctgccc gtatcgtgtc aaggagggag gggcgaggtg ggagggtgat
     3901 ttgacaggca caggactggt ggctataagc caacgcaggc actcatcgtc tgcacatccg
     3961 gccaggtccc agggccggat gggaaaggaa tctatcatgt cttccgagca agagcaggcc
     4021 catcgcttat ttatccgggc gctacaacat gaggcgatta gctgcgagcg cgccggatgt
     4081 ccggggccgg tagagaccct cgaccagtcg caaacacgcg atcgggtcaa gacgtttgat
     4141 atgacctgcg aacgatgcgg gtggcacacc agcgtgaccg ggcgcgaaca gctcacgccg
     4201 ctctgggaca atgcctcatt ggaactgatg gcagatgaac atctgctgca tcaacagccc
     4261 gtatgtccat atgacgacac gccggtcgtg tttatctcca tgccgaatcc gcgccggaag
     4321 gcgaagtacc gggtggcgtg tttttattgt ggccgacaaa ttgaaatgga ttggccaccg
     4381 cctgaaagca aacggtagca acgcggcctg accggaggtg ccggcgggtt agacgaagta
     4441 gcgaccggtg tcgaagtggt actcggcgtt gtcgacgagc tggagaatgt tgtgcgtgta
     4501 ggttccggtg tcatcctgcg cggccaggtc aagctcggga ccatcctcga tcggcgttcc
     4561 ctctgtgtcc gtaatcactc gcaccatcgg acgttccgcg ttggctttgt cctgagcggg
     4621 cttgatgaca accgccaggg gaccggagtc gaggcgcacg aggctcccga tgggaatgat
     4681 gcccacgcag ttcacgaaca gtttgagcag cacggggtcg aacgattgcc cgcttttccc
     4741 gaacatgaat ttgagcacgt ttgctgggga catcggctcc cggcggtata cgcgcgatga
     4801 cgtcatcgca tcgtagcagt cggcgatcgt aatgatgcga ctggcgacgg tttgactcca
     4861 gggcaccttg agtttggggt agcccgaaaa atcatagttc atatgatgtt cgaaggaggc
     4921 cgaggccatg cgcgcgggca cgttgttgat gccccgagcc ttcacgaggg taaacacgcc
     4981 ttcaatcggg tgcgagcgca tgatggccca ttcgtcctgc gtgaactcgc cgggtttgtt
     5041 caagacatcc agagaaatgg cacatttccc tacgtcgtga aacaacgccg acaaaccgag
     5101 atcggccagg tctaccttcg ggtacccggc tcgattgccc agggccatcg ccaggagtga
     5161 cacgttcacg gaatggttat gggtatactg atcgtggcag cgaagattgg tcagtcccag
     5221 gagtgtggat tcatcctgca tcatcagttc gacgatgttc tgaatcgcgc gtttcgcctg
     5281 tttgaagcta accgtccctc cagcgcgcgt actcttcgtc aggtcgccga gcgccgaggc
     5341 cgccttcgca taacgacctt tggcgatggc cttcctcttc tccttcgaat tttcctggcc
     5401 gccggctgag gatcctaccc gcgtccctcc ttcaccggcc attgcgtcgg cgtcgttcga
     5461 ttgctggagt tgaagtgctc gtggttcttc cagttcgata ccgttgacgc ccgccgtctg
     5521 cagggcttcc tgcaaatcgg ccagcgtatg tttctccgga tcgagactga c
//
LOCUS       DAOQDS010000127         5478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_9343_length_5478_cov_20.696109, whole genome shotgun sequence.
ACCESSION   DAOQDS010000127 DAOQDS010000000
VERSION     DAOQDS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9343_length_5478_cov_20.696109"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1252)
                     /locus_tag="PK589_18165"
     CDS             complement(92..1252)
                     /locus_tag="PK589_18165"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNJ21593.1"
                     /translation="MRIRSLANLLVITVLLLISVGLSVALLEFAAGYLYDRAGLANGK
                     RMVDLYLGRTPEMRVGAKEAEKASITPHPYLLFVHTPNMTVDGYQQTNSLGYRNKEFT
                     VEKPADTIRILVVGGSTTFMWPYIKNPADTWVARLEAKLQAISSKQVQVINAGVNYGT
                     SAEALAGYVFRHRFLQPDIVIYHGGGNDVLPLFFDHYNPEYTHFRSAGGSKPRPGEKR
                     LLEHSHTARYLYARWLEPVGSVVVTQPFWEVDPLVALERVRQQAPIGFERNVDSLITL
                     SKSSGADVVLFGFLQAKKQYLSKNAPAFKGYEDALVLGLEKNYEVLARAGSRHHVPFV
                     IPAQDRFQDEWFQDNCHLTPEGEEVKAQIMFEELKTHPQFFSARNPTPPVTH"
     gene            complement(1353..>2943)
                     /locus_tag="PK589_18170"
     CDS             complement(1353..>2943)
                     /locus_tag="PK589_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNJ21594.1"
                     /translation="RKANKSYPATVTLGLDVAYTEAQGTLLHSKKLQSVTTGEVDARA
                     ETCDISGVDKVAKDVISTVVEGVAQQLGTSTKIRELAQSRKAQRGTTGKAASAGQAAP
                     PSPPAPPLSNVPAVPAPVAAATEGTSAPPVSSTGTTHLSFRTILRDQNQNHVLEQNER
                     FSVEFEVRNDGPTPADAVAVDLSGHPAIIASVKTPVSVGLLQPGEIRRVTVEGTVGKV
                     AEAEQAELICTLQAAANVALPSAKKFFVAIRPDATDATEVVSVDVDQLPSKKGKPVQP
                     HAVAIAIGVGAFRDPAMPTMKLATHDAEIMSGYFTSVMGISPQKVKVVLDAKGLKDDW
                     IEVFEQWLPKQVDPQTTAYVYVAGRAMVDHETGAVSLLPYDATLSGSSRAFSLARLQR
                     ALTNAPVKQAVVMLDLSLEPSSGSDPARVAQPRWISPEANGEQERVMLMVGNATVQEA
                     QAYQPGQHGLFTYFLLKGLRGAADLDKNGSVLTGELCAYVQRQVTAVGRSTSGDTQQP
                     LCLPDANGASPLRQLRLSKLP"
     assembly_gap    2944..3043
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3044..3610
                     /locus_tag="PK589_18175"
     CDS             <3044..3610
                     /locus_tag="PK589_18175"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNJ21595.1"
                     /translation="VAAGLVATLNWLTILLSPIALSLAAIYPFCKRWIQIPQAVLGIA
                     FGWGTIMAWAASCNSLAPAAWWLFAATTCWAVAYDTIYALQDREDDRRIGVKSSALLF
                     GTAVPTAVGLFLLGMIVCLIIAGQLAGLGMGYYVALTLLTGVFLWQARRLRFPVTPLQ
                     AFSMFQQHVVAGLVILIALWISASHDLP"
     gene            complement(3612..4625)
                     /locus_tag="PK589_18180"
     CDS             complement(3612..4625)
                     /locus_tag="PK589_18180"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ21596.1"
                     /translation="MRAWRRIRVFGAVVGVVGLSGLIGAGCSMMQSEQAAKGKKLYAH
                     YCMHCHGETGKQNEGFNWSSMSDPKPKDLSNKSEMGTFKDEDIFATISRDMKDTSPNG
                     DKIGDDEFAVPTMPTFKYTLSEEEIWGIVGYVRSLHGKKLEFNVEGRKKELQEAKQSA
                     EQKYKEAERVEQEAEKKASDEADKKGVEVDDNAYAKEMQAMGQAKKELDQATAALANF
                     TTRPGKGVNIARPDLNMKPDAAAKLVEVGKQIYVTKYGCNGCHKIGEDGGKVGPALDR
                     AGYRLNPTWVYRWLRNPQAMKPDTRMPSLGLNDADAKAVALYLKTLRAPKPDKPLGKV
                     GEE"
     gene            complement(4628..5455)
                     /locus_tag="PK589_18185"
     CDS             complement(4628..5455)
                     /locus_tag="PK589_18185"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNJ21597.1"
                     /translation="MARVRRVGKRWWVGAVGLLGLCVAAEVQPAYSQQSVAVRAALVK
                     GSLPADDPTAAAWATASVSEFPMSPQVHWPVRITEVTAKSVKVRGLHDGTTVAIMLEY
                     SDPSEDPDDAAALEFMVGDTKAHFAHGQPMAQVEGGPVNIWYWKNKDGKGSDLGAKGF
                     GTLKPHSHQDVKAKGVYQGGVWKVVFSRPLSTEHVAEDTQFKPGEFGSIAFAVWDGKK
                     TESGQPKEKGSEKAISSWWYFRADAPPDYSPYLYALLAVGLALGFEMALVRRLKKGS"
BASE COUNT         1031 a         1625 c         1471 g         1251 t
ORIGIN      
        1 acctgcggtt tacgaaaccg ctgctctgcc aactgagcta cgccggccag gcggggtgag
       61 gcgcaatatt actcagacgg tgaaacacct gtcaatgtgt gacggggggt gttgggttcc
      121 ttgcgctgaa aaattgagga tgggtcttca gctcctcgaa catgatctgc gctttgacct
      181 cttctccttc cggtgtcagg tggcaattgt cctgaaacca ctcatcctgg aatcgatcct
      241 gagcaggaat gacaaagggc acgtgatgcc gactcccggc ccgtgctaga acctcataat
      301 ttttctccag cccaagtacg agcgcatctt cataaccttt aaatgccggg gcattcttac
      361 tcagatattg tttcttcgcc tgcaaaaacc caaagagcac cacatccgcc cctgaactct
      421 tgctcagagt gatcagagaa tccacgtttc gctcaaaccc gatcggggct tgctgccgaa
      481 cccgttctaa tgcgacgagc gggtccactt cccaaaatgg ttgcgtcacc accaccgacc
      541 cgaccggttc aagccacctg gcataaagat atctggcggt atggctgtgc tcaaggagcc
      601 gcttttctcc cggtctcggc ttactcccac cggcgcttct gaaatgcgta tactcagggt
      661 tgtaatggtc aaaaaataac ggcagcacat cgtttcctcc gccatggtaa atcacgatgt
      721 ccggctgaag aaaccgatgc cggaacacgt acccggctaa ggcttccgca ctcgtgccat
      781 agttgactcc ggcattgatg acctggactt gtttgcttga tatcgcttgg agcttcgctt
      841 ccaggcgcgc cacccatgtg tcggcaggat ttttgatgta cggccacatg aacgtggtgg
      901 acccgcccac aaccagaatc cgaatggtgt cggccggttt ctccaccgta aattctttat
      961 tgcgataccc gagtgaattc gtttgttggt acccgtctac ggtcatgttg ggggtatgca
     1021 caaacagcag atacggatgc ggcgtgatcg aggccttctc cgcttctttt gcacccacac
     1081 gcatctctgg cgtccggcct agatagagat ccaccatccg ctttccattc gccagtcccg
     1141 cgcggtcgta gagatagccc gcggcaaatt ccaacagcgc aacggatagg ccaacgctga
     1201 tgagcagcag caccgtgatg acaagaagat tggcgaggct tcgtattcgc atggtgtgcc
     1261 gcgagcacaa gcaaccagag agcgaaggac cggacgaacc acgaatgaat cgttcagtac
     1321 gctagaccac tattggaacc ggtgacgcgc cgtcagggca gcttactaag acggagctgt
     1381 ctaaggggag acgcgccgtt ggcgtccgga aggcagagcg gctgttgggt atcccccgat
     1441 gtcgaccgcc caacggccgt aacctgtcgc tgcacgtagg cgcacaattc tccggtgagc
     1501 actgatccgt ttttatcgag atcagctgcg ccgcgaagtc ccttgaggag aaaatatgtg
     1561 aagaggccat gttggcctgg ttgatacgcc tgagcttcct gcacggtggc attgcccacc
     1621 atcagcatca cacgctcctg ctccccgttg gcctccggcg aaatccaccg cggctgggcc
     1681 acgcgcgcag ggtccgaacc ggacgagggc tccagtgaga gatccaacat cacgacggct
     1741 tgtttgacag gcgcgttcgt cagtgcgcgc tgcaaccggg ccagagagaa cgcgcgggaa
     1801 gagccggata gcgtggcatc atacggtagc agcgatacgg caccagtctc atgatccacc
     1861 attgctcgtc cagcaacata cacgtaggcc gtggtctgcg ggtccacctg cttgggtaac
     1921 cactgttcga atacctcaat ccaatcgtct tttagtccct tggcatccag cacaactttg
     1981 accttctgcg gcgaaattcc catcacggac gtgaagtacc cgctcatgat ttcggcatcg
     2041 tgcgtggcca gtttcatcgt aggcatggcc ggatcgcgaa acgcaccgac tccgatcgca
     2101 atcgcgacgg catgcggttg aaccggtttc ccctttttgc tggggagttg atcgacatcc
     2161 acggagacga cttcggtcgc atcggtcgca tccggtctga tggccacaaa aaactttttt
     2221 gccgacggaa gcgcaacgtt cgcggcagcc tgcagggtgc agatcaattc tgcctgttcc
     2281 gcctctgcta ccttaccgac agtgccctct accgtgacgc gccgaatttc tcctggctgc
     2341 aacagcccca cggagacagg cgtcttcact gaggcgataa tggcaggatg cccgctgaga
     2401 tccaccgcaa cggcgtcggc cggcgttggc ccgtcgtttc tgacttcgaa ttccacgctg
     2461 aagcgttcat tctgctccag gacgtggttt tggttctggt ctcgcaaaat ggtgcggaat
     2521 gacaggtgcg ttgtgccggt cgaggagact ggcggtgcag aggtcccttc agtcgcagct
     2581 gccaccggcg ctggaacggc cggcacattg gacaaaggcg gagcaggcgg agagggaggg
     2641 gcagcctgcc ctgccgaggc ggccttgccc gtcgttcctc gttgagcttt tctcgactgg
     2701 gcgagctccc ggatttttgt ggaggtgccg agctgttggg ccacaccttc cacaaccgtg
     2761 gagatgacat ccttggccac cttatcgacg ccactgatgt cacaggtctc cgcccgcgca
     2821 tcgacttccc ccgtggtcac gctttgtaac ttcttactat gcagcagagt gccttgcgcc
     2881 tcggtgtacg ccacatcgag tcccagcgtc accgtggcag ggtaggattt attggctttg
     2941 cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtagcgg caggcctcgt
     3061 ggccacactc aactggttga cgatactact cagccctatc gccttgtccc tagccgcgat
     3121 ctatccattt tgcaaacgat ggattcagat tccgcaggcc gtattgggaa tcgccttcgg
     3181 atggggaaca atcatggcct gggccgcatc ctgcaacagc ctcgcgccgg ctgcctggtg
     3241 gctgtttgcc gccacgactt gctgggcagt ggcctacgat acgatttatg cgctgcagga
     3301 ccgggaagat gatcgccgca tcggagtaaa gtcttcggcg ctgctttttg gaacagccgt
     3361 gcctaccgcc gtcggcctgt ttttgctggg aatgatagtc tgcctgataa tcgcgggaca
     3421 gctggctggt ctagggatgg gatactatgt ggccttaacg ctgctgacgg gagtctttct
     3481 ctggcaagcg cggcgcctac ggtttcccgt gacgccgctt caagccttca gcatgtttca
     3541 gcagcatgtc gtggctggtc tcgtgattct gatcgcgttg tggattagcg cgtcacacga
     3601 cttgccctaa gctattcctc tccgacctta ccgagcggct tgtcgggttt cggagcacgg
     3661 agtgttttca gatagagcgc caccgctttg gcgtcggcgt cgttcagacc caagctgggc
     3721 atgcgcgtat ccggcttcat ggcttgcggg ttacgcagcc accggtacac ccaggtcgga
     3781 ttcaatcgat agcctgcccg atccagagca ggaccgacct ttccaccgtc ttctccgatc
     3841 ttatggcatc cgttgcagcc atacttggtg acatagattt gcttgccgac ttcgacgagt
     3901 ttcgcggcgg cgtccggctt catgttcaag tcggggcgcg caatgttcac gcctttgcct
     3961 gggcgggtcg tgaaattggc cagtgcggct gtggcttgat caagctcctt tttggcctgc
     4021 cccatcgcct gcatttcttt ggcataggca ttgtcgtcga cttccacgcc cttcttgtca
     4081 gcttcgtcgc tggccttctt ctcggcctcc tgctctacac gttccgcctc tttatacttt
     4141 tgctcggcag attgcttggc ctcttgaagc tctttcttac gaccctcgac gttaaattcc
     4201 aattttttgc cgtgcaatga ccgcacgtag ccgacgatgc cccaaatctc ttcttccgac
     4261 agcgtgtatt tgaaggtcgg catggtcggc accgcaaact cgtcgtcgcc gatcttgtcg
     4321 ccgttcgggc tcgtatcctt catgtcccgg gagatcgtgg cgaaaatatc ttcgtccttg
     4381 aatgtgccca tttcggactt attggacaga tcctttggtt tcgggtcact catagaggac
     4441 cagttaaacc cttcattctg ctttccggtt tcaccgtggc agtgcatgca atagtgcgcg
     4501 tagagcttct tgcccttggc ggcctgttca ctctgcatca tcgaacaacc ggcaccgatg
     4561 aggcccgaga ggcccacgac cccgacgact gctccaaaga cgcgtattct tcgccacgcc
     4621 ctcatgctca cgaccctttc tttaaacgtc tcacgagtgc catctcaaag cccagcgcga
     4681 ggcccaccgc gagtaatgcg tacaggtacg gggaatagtc cggtggtgcg tcggcacgga
     4741 aataccacca ggatgaaatc gctttttcgg accctttttc ttttggctgg ccgctttccg
     4801 tctttttgcc gtcccagaca gcgaacgcga tgctgccaaa ttctccgggc ttgaactgag
     4861 tatcttccgc aacatgctcc gtgcttaacg gccgtgagaa gacgaccttc catactccac
     4921 cctggtagac gcccttggcc ttgacgtcct gatggctgtg aggcttcagc gtgccgaacc
     4981 ctttcgcgcc caggtcggaa cctttgccgt ctttattttt ccagtaccag atattgaccg
     5041 ggccgccctc gacctgtgcc atcggctgac cgtgagcgaa gtgcgccttg gtatccccga
     5101 ccatgaactc taaggcggcc gcatcatcgg gatcctcaga gggatcggag tactccagca
     5161 taatggcgac tgtggtccca tcgtgaaggc cgcgaacctt gacacttttc gcggtcacct
     5221 ccgtgatccg aaccggccag tggacctgag gggacatagg gaattcagac acggaggcag
     5281 tggcccatgc cgctgccgtg ggatcatcgg caggaaggga gcccttcaca agtgccgccc
     5341 gcactgcgac gctctgctga ctataggcgg gctgcacttc ggcagccacg cacagcccta
     5401 gaaggccgac ggctccaacc caccatctct ttcctactct cctcactcgt gccatacgtt
     5461 ccccttaatt gctcagca
//
LOCUS       DAOQDS010000128         5418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_9544_length_5418_cov_20.588663, whole genome shotgun sequence.
ACCESSION   DAOQDS010000128 DAOQDS010000000
VERSION     DAOQDS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9544_length_5418_cov_20.588663"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PK589_18190"
     CDS             complement(<1..190)
                     /locus_tag="PK589_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21598.1"
                     /translation="MPHAASDRCLGMVNQMNDLAVRVEHLHKRYRLGSTHEHDGSLAG
                     ALSRGMRQLVGRRSAPPPT"
     gene            complement(207..473)
                     /locus_tag="PK589_18195"
     CDS             complement(207..473)
                     /locus_tag="PK589_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNJ21599.1"
                     /translation="MELHDRLEEVFRQVFDNEALELRDQMKAADIEGWDSVAHINLMF
                     GIEQAFGIRFKGNELADMKNIGELKDFLAGKLNGEASPMRKVLP"
     gene            complement(559..2106)
                     /locus_tag="PK589_18200"
     CDS             complement(559..2106)
                     /locus_tag="PK589_18200"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ21600.1"
                     /translation="MARPDLSSSTQCFLPHMITVRFTKQDLARFSAASRDRNPLHISE
                     DYARTTPYAEPVVFGVLSLLAGLGHLSTRHGKQLQHLSVEFRNPLSLDVPYCLDIIES
                     STDNARVKLYDTTRLMMTATFAFMPGQGNPESRRFSETSCATEPEDRKKDSLLPGTRV
                     TGTYAPHTEAFAQVVDRWELEGKGATPYQLAAMMWASYVVGMHLPGKRAVLWRLTLDF
                     HDVEPPGQEPFSYEATVEDLDERYDLLRCRGTLSAGSLPYATAHMSAFVRQDSPRSSL
                     RRISDLLPESEQLKGKRALIIGGSRGLGAAIAQALISQGCSVLLSYQQSTAEAEQLRA
                     SVGDRSTQLELVQGDAADIEWCLSLRDMILSRYGGLDLLVCNASPPIRPLAFEPEKFT
                     QFQDFLTKSLALVSAPMSTFLGLLAHQAGWNIILSSSYVKELPAAFAHYVTAKCALEG
                     LVNWAAVQYPTVRHRIVRPPKLLTDQTNTTIGRQGAMDVEQVAVSIVRQICQPHQPAA
                     VQIMETF"
     gene            complement(2093..4114)
                     /locus_tag="PK589_18205"
     CDS             complement(2093..4114)
                     /locus_tag="PK589_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HNJ21601.1"
                     /translation="MSQQSMTPTGIVSPQQLTSSDTPETRGDAYRKQGQLEQAIRCYL
                     EAVTFPLSPALCLKLARCYDRLDHHSEACRWALSLVEAGDDYTTWLAGWTLFQRHAGQ
                     GGWSALRSTKVAMVSSYTTTQFCPLLQLAAARVGIHIDLYESQYGQYQQDIIDPGSRL
                     YAFAPDIVILAVHEGDLRLPEYSSTPDQDIQAEVDRWTTLWKLVAERAQARVVQHNFA
                     LPCEVPAGHLATRLPGSRYRMTQAVNMRLGDSAGLGVSLVDCERLSGLIGKRQWCDPR
                     YWNLSKQAVALEALPLLARHTAAVIAADLGLSRKCLVLDLDNTLWGGVIAEEGLAGIK
                     LGQGPEGEAFVAFQEYLLKLKRKGVILTVCSKNNYADAIHPFEKHPEMRLRLQDIALF
                     IANWESKPDNIRRIADTLQIGLDSLVFVDDNPVERDIVRKFLPEVDVLPLPDDPAYYT
                     RALSQYLSLETASLTAEDSERADQYRARAQIKELEAAAGSIEDFYLSLRMQAIVTPFE
                     ESQLPRIAQLIGKTNQFNLTTRRHGMPQLTAFIQSTDYVHLALRLRDRYTDHGLVSVM
                     IAQCRGEVLDIDTWLMSCRVIGRTVEATMLELLCRHAAQLGCTILQGTYIPTQKNAMA
                     ADVYAKHGFEPFNQTNGHHVWRYDLTANGPIANPFVKRVDAWESVDGTP"
     gene            complement(4111..4749)
                     /locus_tag="PK589_18210"
     CDS             complement(4111..4749)
                     /locus_tag="PK589_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNJ21602.1"
                     /translation="MNVLARILHPTDMPMVGTFRRLYRSVQDLAGQIYEDWATNNHDW
                     TMPGFRAIAVHRFGTWVSNKRPGALRTVLLWLYQVMYRYIRNYYGIEIPLTVILGRRL
                     WLVHQNGIVFHWKTVVGDDCLIRHNATIGAGYGGQKTLHKGPRLGNHVEVGPGAVIFA
                     AVAIGDGAVIGPNVVVMSNVPAGARVFAEAPRLIRLANAPDAHIVEGMSQNR"
BASE COUNT         1125 a         1503 c         1622 g         1168 t
ORIGIN      
        1 cggtgggcgg cggggcagag cggcggccga ctaactggcg catgccccgc gataaagccc
       61 cggcgagaga tccgtcatgc tcatgggtag agcccaatcg ataccgcttg tgcaaatgtt
      121 cgacgcgaac cgccaggtca ttcatctgat tcaccattcc gaggcagcga tcgctagctg
      181 catgtggcat gctccgcaga tgcgccttag gggagaacct ttcgcatggg ggacgcttcg
      241 ccgttcaact tgccggccaa aaaatccttc agctcgccga tattcttcat gtcggccaac
      301 tcattgcctt taaatcggat tccgaacgcc tgctcgatgc cgaacatcag attgatatgc
      361 gccacggaat cccagccttc gatatcggcg gctttcatct gatcgcgcag ctcaagcgcc
      421 tcattgtcga atacttggcg aaagacttct tccaatcgat cgtgcagctc catgaactcc
      481 tcacatcatg cggttaggtg tcgtatggtc attggtcggg taaaagactt gtgcctgtta
      541 ggtcgttgtc atggcccgtc aaaatgtctc catgatctgc acggccgctg gttggtgggg
      601 ctgacagatc tgtcgcacaa tggagaccgc gacctgttcg acgtccatgg ctccctgccg
      661 tccaatggtt gtgttggtct gatcggtcaa aagtttgggc ggcctgacaa tacgatgccg
      721 aacggtcggg tattggacgg cagcccagtt caccaagcct tccagcgcgc attttgccgt
      781 cacgtaatgc gcgaaggctg ccggaagctc tttcacatag gatgacgaca ggatgatgtt
      841 ccatcctgcc tgatgagcca gcaagccgag aaacgtggac atcggcgcgg agaccagtgc
      901 cagacttttc gtgaggaagt cctggaactg cgtgaatttt tccggttcga atgcaagcgg
      961 acgaatcggg ggcgaggcat tgcagacgag aaggtcgagg cctccgtacc gtgagagaat
     1021 catgtcccgt aaagacaggc accattcaat atctgcggcg tcgccctgca ccagttcaag
     1081 ctgagtcgac cggtcaccca cactggcgcg aagttgctcc gcttccgccg tactctgctg
     1141 gtagctcagc aacacggagc agccctgtga aatgagcgct tgcgcgatgg cggcgccaag
     1201 accccggctt cccccgatga tcagcgcgcg ctttcccttc aattgttcgg attccggtag
     1261 caggtcgctg atgcgtcgaa gcgaagatcg aggagaatcc tgtcgcacga atgccgacat
     1321 gtgagccgtc gcatagggga gcgagccggc agaaagggtg ccccgacatc gcaagagatc
     1381 atatcgctca tcgaggtctt caacggttgc ctcgtaggag aacggttctt ggcctggtgg
     1441 ttctacatcg tgaaaatcaa gcgtcagccg ccagagcacc gcgcgtttgc cgggaaggtg
     1501 catgcctacg acataactcg cccacatcat ggccgcgagc tggtatgggg tcgcgccttt
     1561 tccctcgagc tcccagcggt cgaccacctg cgcgaacgct tcggtgtgtg gcgcataggt
     1621 gccggtcacg cgagtgcccg gaaggaggga gtcttttttt cgatcttccg gctccgtagc
     1681 gcaagatgtc tccgagaagc gccgtgattc aggattgccc tgtcctggca tgaaggcgaa
     1741 tgtcgccgtc atcatcagac gagtcgtatc atagagtttg acgcgtgcgt tgtctgtcga
     1801 cgattcgatg atgtcgagac aatagggcac gtcgaggctg agaggattac gaaattcgac
     1861 cgagagatgc tgcagctgct tgccatggcg tgtcgaaaga tggcccaatc ccgccaggag
     1921 actcaacacg ccgaacacca ctggttcggc ataaggcgtc gtgcgggcat agtcctcgga
     1981 gatgtgcagg ggatttcggt cgcggcttgc cgcgctgaag cgtgcaagat cctgtttcgt
     2041 aaagcggacg gttatcatat gaggcaggaa gcattgagtc gaactcgaca agtcagggcg
     2101 tgccatcaac ggattcccat gcatcgacgc gtttcacaaa cggattggca atcgggccgt
     2161 tcgctgtcag gtcataccgc cacacgtgat gcccattggt ctggttgaag ggctcgaaac
     2221 cgtgtttcgc atagacatct gctgccatcg cgttcttctg ggtcgggata tacgttcctt
     2281 ggaggatagt gcagccgagc tgggcggcat gccggcagag gagttccagc atggtcgctt
     2341 ccacggtgcg gccgatgacc cggcaactca tgagccaggt gtcgatatcc agaacctcgc
     2401 cccggcattg cgcaatcatg acactgacca ggccatgatc ggtgtagcga tcccgcagcc
     2461 gcaaggcgag gtgcacgtag tcggtgctct gaatgaaggc ggtcaactgc ggcatgccgt
     2521 gtcgtctggt ggtcagattg aattggttgg tcttgccgat cagttgcgcg attctcggca
     2581 gctgtgattc ttcgaagggc gtcacgatcg cctgcatgcg gaggctgaga tagaaatcct
     2641 cgatcgaccc ggccgcagcc tccaactctt tgatctgcgc cctggctcga tactggtcgg
     2701 ctcgctcact atcctcggcc gtcaaagagg cggtttcgag cgacaggtac tgcgacaggg
     2761 ccctggtgta ataggccgga tcgtcaggca acggaaggac atcgacctcg ggaagaaatt
     2821 tgcggacaat gtcacgttcg accgggttat cgtcgacaaa cacgagacta tccagcccga
     2881 tctgtagggt gtcggcgatc cggcggatgt tgtccggctt ggattcccaa ttcgcgatga
     2941 aaagggcgat gtcctgcagc ctgagccgca tctcaggatg tttttcgaac ggatgaatgg
     3001 catcggcata gttattcttt gagcagacgg tcaggatgac gcctttccgt ttcagcttga
     3061 ggagatattc ctgaaatgcc acgaatgcct cgccttccgg tccctgtcct aatttgattc
     3121 cggcgagacc ttcttccgcg atcactccgc cccataaggt gttgtcgaga tcgagtacga
     3181 ggcacttacg gctcaagcca aggtccgcgg cgatcacagc cgctgtgtgt cgtgccagca
     3241 acggtagcgc ctcgagcgcc acagcctgtt tggaaaggtt ccagtatcgc ggatcgcacc
     3301 actgccgttt tccgatgagc cccgacagac gttcgcaatc caccagggac acgccaagtc
     3361 ctgccgaatc tcctaaccgc atattcacgg cctgcgtcat cctgtagcga gagccgggaa
     3421 gtctggtcgc gagatggccg gcgggcacct cgcagggcag cgcaaagtta tgctgtacga
     3481 cgcgggcttg cgcccgttcc gcgaccagct tccagagtgt cgtccaacgg tcgacctcag
     3541 cttgaatgtc ctggtcaggc gttgagctgt actcggggag ccggagatct ccttcgtgaa
     3601 ccgcgaggat gacgatatcg ggagcgaacg cgtacaggcg gctccccgga tcgatgatgt
     3661 cctgctggta ttgtccgtac tggctctcgt agagatcgat atgaatgccg accctggcag
     3721 cggcaagctg taataacggg cagaattgag tcgtagtgta gctactcacc atggcgacct
     3781 tggttgatcg caatgcagac catccacctt ggccggcatg acgttggaag agcgtccagc
     3841 ctgcgagcca ggtcgtgtaa tcatcacccg cttcaaccag ggaaagggcc catcgacagg
     3901 cttcggaatg gtgatcgaga cgatcgtaac atcgcgcgag tttcaagcag agcgcgggag
     3961 agaggggaaa ggtcaccgcc tcgagatagc agcgaatggc ctgttccagt tggccttgct
     4021 tgcgataggc gtcaccgcgt gtctcgggcg tgtcggaaga cgtgagctgt tgtggcgaga
     4081 cgatgccggt aggggtcatg ctctgctggc tcacctgttt tgagacattc cctcgacgat
     4141 gtgggcgtcg ggggcattgg ccagccgtat cagacgggga gcttcggcaa agactcgggc
     4201 tcctgcggga acattcgaca tcaccaccac gttcgggcca atcactgcgc cgtctccaat
     4261 ggccacggcg gcaaaaatca ccgcgccggg acctacttcc acgtggttcc cgagcctggg
     4321 gcccttgtgc aatgttttct gtccgccata accggcgccg atggtggcat tgtgacggat
     4381 cagacagtcg tcaccgacaa ccgtcttcca atgaaacacg atgccatttt gatgcaccag
     4441 ccacagtctt cgccccagga tgacagtcag aggaatctca atgccgtagt agttccgtat
     4501 gtagcggtac atgacctggt acaaccagag cagtacggtc cgaagcgccc cgggtcgttt
     4561 attggaaacc caggtgccaa agcgatggac ggcgatggcg cgaaatccgg gcatcgtcca
     4621 gtcatggttg ttggtggccc agtcttcgta aatctggccc gccagatcct gcacgctgcg
     4681 atagagtctg cggaacgttc ctaccatggg catgtcggtt ggatgaagga ttctcgccag
     4741 aacgttcata gcgcctcatt tcgtcgcact gccattcttc aacagtccaa ggcagtgctg
     4801 tgggcagatg ggctagctcg cacactcgac ccttccgcgg caatcacttc ttccatcgtc
     4861 ggactgcgga tcagcagtct tgttggtcgg tcatgatgat cgtcgggatc aatagtgaac
     4921 ggtgctgctc acacagatcg atgtccctcg atcagccacg ttcttccggc ggctggtgaa
     4981 tgtagccaat cttatcatat gtccagcagt actggacaag cccaaaacgc cgtcgatcaa
     5041 caaattcgtg atgatgaaag gatgtttgtg tagatctcgc gacgacaatg aaccacgcga
     5101 ggaggtgaga gacggagtcg gttgcggagc attccagggt ggcggacccc tgtttatcag
     5161 cattggacaa acagaagcgg aaatcgacta ggtatatccc tagtgccggc agactgtggc
     5221 gtgtcagctg ctgatgaacg gcgcaaacgg agggcaagcg agagcgggtc atcatattgg
     5281 acagtcgtcc gtcaactctg ggccgaaatg cctatttcat ggcttgtatg gcaatggggc
     5341 ccgtggtact attcgcggtc tcccgctggg gcctggtgag ggatgcgttg aaatagttca
     5401 ggcctgactc gatgttgc
//
LOCUS       DAOQDS010000129         5398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_9609_length_5398_cov_28.167509, whole genome shotgun sequence.
ACCESSION   DAOQDS010000129 DAOQDS010000000
VERSION     DAOQDS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9609_length_5398_cov_28.167509"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..76)
                     /locus_tag="PK589_18215"
     tRNA            complement(3..76)
                     /locus_tag="PK589_18215"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Gln,seq:ttg)
     gene            complement(130..203)
                     /locus_tag="PK589_18220"
     tRNA            complement(130..203)
                     /locus_tag="PK589_18220"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(169..171),aa:Gln,seq:ctg)
     gene            complement(271..1686)
                     /gene="gltX"
                     /locus_tag="PK589_18225"
     CDS             complement(271..1686)
                     /gene="gltX"
                     /locus_tag="PK589_18225"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNJ21603.1"
                     /translation="MSQVRVRFAPSPTGFLHIGGVRTALFNWLFARQQNGVFILRIED
                     TDQDRSTDASIQAILDGMRWVKLDWDEGPFRQTERMDLYRSHALRLFEQGHAYWCVCT
                     PEELEARRKEAEAKGGSPRYDGRCRNLGLAKPTSDAALRFKAPQDGQTVVDDLIKGRV
                     VFDNQILDDVIILRSNGFPTYNFSVVVDDALMGITHVVRGDDHLTNTPRQIPIFQALG
                     FPLPRFGHLPMILGSDKTRLSKRHGATSIMAYKEMGYLPEAMVNYLVRLGWSHGDQEL
                     FSRDELIEKFSWKNVQTSPAVFNPDKLLWLNAEYLKTNPPQQVAEALVPLLEQTDLKD
                     EVRAVSTEWLAQLVVLVKERTKTLVEMVHWVRPYFGEAVSFDEEAAKKFLTPAQAPIL
                     TKLVERFEALPVFSKHEWEEAFKKLVEEAGIKMGQLAQPVRVALTGRTASPGLFEVME
                     VLGRERTLIRLRKGIEQAARS"
     gene            1804..2502
                     /locus_tag="PK589_18230"
     CDS             1804..2502
                     /locus_tag="PK589_18230"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ21604.1"
                     /translation="MAEPTQLAEVVSLTALTPHTTELVLRPIESPLPFKPGQWISLQL
                     PIGDHPPLNRAYSLAEPPTSKGHLTLVFDHVPGGQGSGYLSSLKPGDRLPLSGPYGHF
                     VLPEPEMQHLLLIGRYSGLVPLRCMIRALAEQKTLPATTLIAHAPTASEQLYHDEFTA
                     LAARHSHFQYLPFVSETPDGHVGTLEVIKQLAGNGRRVTPMIAGIKAFARPLRSLFMD
                     LGFDRREVKLETYD"
     gene            complement(2530..2946)
                     /locus_tag="PK589_18235"
     CDS             complement(2530..2946)
                     /locus_tag="PK589_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNJ21605.1"
                     /translation="MGGTNPYIEKADVELPSKAYTVTFVFPDKTEKKVEVQPDKIPYG
                     PTGLPGSILDIAMGNDIDLEHVCGGVCACSTCHVMVKKGLETCNEGTDDEFDQLDEAP
                     ATTLQSRLGCQCVPNGTTDVTVEIPAINKNLAKEGH"
     gene            3160..3639
                     /locus_tag="PK589_18240"
     CDS             3160..3639
                     /locus_tag="PK589_18240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNJ21606.1"
                     /translation="MLKLSKKADYGLMALQHIASNQYGDVAHARVVNTKEIAEEYHIP
                     VELLAKVLQTLSKSGIIESHNGPKGGYLLGKNPREITIAHVLESLEGPLGIMDCSHEK
                     DGDACMQREHCNIRTPLLKIQNSIYQLLNNMTLQDMLGGTPLITIQSVATEQQGVER"
     gene            3636..4853
                     /locus_tag="PK589_18245"
     CDS             3636..4853
                     /locus_tag="PK589_18245"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNJ21607.1"
                     /translation="MKFPIYLDNHATTPMDPRVLETMLPYFTEKFGNAASRNHAFGWE
                     AEEGVETARKQIAKLIHADAKEIVFTSGATESNNLALKGVVEMYHEKGDHIITSSTEH
                     RAVLDTAKTLETKRGVKVTYLPVDKFGMVNPEDVRNAITDKTILISVMFANNEIGTIN
                     PIKEIGKIAKEKGILFHCDATQGVGKVPINVQEMGIDLMSFSAHKIYGPKGIGALYVR
                     KKNPRVRIAAQMDGGGHERGMRSGTLPVPLIVGFGKACELCEQEMAADAARLSVMRDR
                     LHATITKALEDVYLNGHPTERLPHNLNISFAYVEGESLLMGCKEIALSSGSACTSATL
                     EPSYVLRALGVGAELAHSSIRFGLGRFTLDEEVDYAAKKIIETVTKLREMSPLYEMAK
                     EGIDLKTVQWAAH"
     gene            4894..5277
                     /gene="iscU"
                     /locus_tag="PK589_18250"
     CDS             4894..5277
                     /gene="iscU"
                     /locus_tag="PK589_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HNJ21608.1"
                     /translation="MAYSEKVVDHFNNPRNMGSFKKEEDGVGTGIVGAPECGDVMKLQ
                     IKVQNDTIVDAKFKTFGCGSAIASSSLATEWLKGKTVEEAGKIKNTDIVQELNLPPVK
                     IHCSVLAEDAIKAALADYQKKADSK"
BASE COUNT         1267 a         1562 c         1444 g         1125 t
ORIGIN      
        1 tttggctggg ggactaggaa tcgaacctag gtagccagct ccaaaggctg gcgtcctacc
       61 gctagacgat cccccagcgc ggtacggaac cgagacatcg agctgaagag caaggcgtgg
      121 aaaagatttt ggctggggga ctaggaatcg aacctaggta gtcagatcca gagactgacg
      181 tcctaccgct agacgatccc ccaaccggtc cacacagtaa tataagcctc tccattgcgt
      241 caagatcgag cgtgattctt gaacagccaa tcaggatctg gcggcctgct cgatcccctt
      301 gcgcaatcga atcaacgtac gttcccggcc gagcacttcc atcacttcga acaagccggg
      361 actcgccgtc cggccagtca aggcaacccg caccggttgg gccaactgcc ccattttgat
      421 tcccgcttcc tcgaccaact tcttgaacgc ctcttcccac tcgtgcttgg aaaagactgg
      481 aagcgcctca aagcgttcca ccagcttagt caatataggg gcctgagccg gagtcaggaa
      541 ttttttcgcg gcctcctcat caaacgacac cgcctcgcca aagtaaggcc tcacccaatg
      601 aaccatttca accaatgtct tagtccgctc tttcaccaag accacgagct gcgcgagcca
      661 ctccgtcgag accgcgcgga cctcgtcttt gaggtcggtc tgttcgagca acggcaccag
      721 cgcttccgcc acctgctgag gcggattggt cttaagatat tcggcattga gccacagcaa
      781 cttgtcaggg ttgaagacag ccggagaggt ctggacgttc ttccaggaga acttctcgat
      841 cagttcgtct cgcgaaaaga gctcttgatc cccatgcgac cacccaagcc ggacgagata
      901 gttgaccatg gcctccggca gatagcccat ttccttgtaa gccatgatcg acgtcgcgcc
      961 gtgacgcttc gacagccgcg ttttgtccga acccaggatc atcggtaagt ggccgaagcg
     1021 tggcagcggg aatccgaggg cctgaaaaat ggggatttgg cgcggggtgt ttgtgagatg
     1081 gtcgtccccc cgcaccacgt gtgtgatgcc catcaacgcg tcgtccacca ctactgagaa
     1141 gttataggtg ggaaagccgt tcgacctgag aatgatgacg tcatccagga tctgattgtc
     1201 gaacaccacc cggcccttga tcaggtcatc aacaaccgtc tgaccgtcct gcggcgcttt
     1261 gaaccggagc gccgcgtcac tggttggctt ggcgagaccc aggtttcggc accgaccgtc
     1321 atatctgggt gatcctccct tggcctccgc ttctttccgg cgagcctcca gttcctccgg
     1381 tgtgcacacg caccagtagg catggccctg ctcaaacagc cgcagagcat ggctacgata
     1441 gagatccatg cgttcggtct gccgaaaggg accttcatcc caatccagtt tgacccagcg
     1501 catgccatcg agaatagctt ggatcgatgc atcggtcgac cgatcctggt cggtatcttc
     1561 gatgcggaga atgaacacgc cgttctgctg ccgcgcaaac agccagttga acaaggccgt
     1621 ccgcaccccg ccgatatgga ggaagccggt cggactgggc gcaaatcgaa ccctgacctg
     1681 actcatcgcg tcaccgtagt gtgtgtggga gaaattaacg ggggggtatc tatatcatgg
     1741 caccggaacg atgtacagaa cggggctgtt ccattctgaa tctgtttttg ctaagaacag
     1801 tccatggctg aaccaacaca acttgctgaa gtcgtctcgc taacggcgtt gactccccat
     1861 accaccgaac tcgtgctccg tccgatcgag tctcccctgc ccttcaaacc cggccagtgg
     1921 atctcgctcc aattgccgat cggagaccac cccccattga atcgggccta ttctttggca
     1981 gagcccccga cctccaaagg acatctcaca ctggtgttcg atcatgttcc gggaggtcaa
     2041 gggtcgggct atctttcgtc actcaagccc ggggatcgcc tccccctctc ggggccctat
     2101 ggtcatttcg tgttgccaga gccggaaatg caacatttgc tgttgatcgg tcgttattca
     2161 ggccttgttc cactgcgctg tatgatccgc gcactggcag agcagaagac gttgcccgcg
     2221 accaccctga ttgcgcacgc ccccaccgcc agcgagcaac tctatcatga tgaattcaca
     2281 gcgctggcag cgaggcactc ccattttcag tacctgccgt ttgtgagcga gaccccggac
     2341 gggcatgtgg ggactctgga ggtgatcaaa caactggctg gaaacggccg ccgcgtcacg
     2401 cccatgatcg cggggattaa ggcctttgcc cgcccgttgc gcagcctgtt tatggacctg
     2461 gggtttgatc gacgggaagt gaaactcgaa acgtacgatt gaagcctcat caggctgtgt
     2521 cttgctgaat caatgacctt ccttcgccag attcttattg atcgcgggaa tctccacggt
     2581 gacgtctgtc gtcccattcg gcacgcactg gcacccaagc cgggattgca gcgtcgtggc
     2641 gggagcctcg tctaactgat cgaactcatc atccgtcccc tcattgcacg tctccagacc
     2701 ctttttcacc atgacatgac acgtggagca ggcacagacc cctccgcaca catgctccag
     2761 gtcgatgtcg ttccccatgg cgatatccag aatgctgccc ggtaggcccg tcggaccgta
     2821 gggaatcttg tccggctgca cttccacctt cttttcggtc ttgtccggga atacgaacgt
     2881 gacggtatac gccttcgacg gcaattcgac gtctgctttt tcaatatagg gattcgttcc
     2941 acccatggtg cgcagcctct tttcttttca gtttattttc aatgacaagt gcaatggacg
     3001 atcagccgac catcgggccg cgccctcggg aagccacctc tttctacact ttggctcacc
     3061 ggcgcccttg acagtataaa agatcacatg atagcttttg tacgactcat ttgatcggag
     3121 atcattatag tctccgactt taaagatcgg caataggcca tgctgaaatt atctaaaaaa
     3181 gcagactacg gattaatggc actgcagcac atcgcctcca accagtacgg cgatgtcgct
     3241 catgcccgag tggtgaacac caaggaaatc gccgaggaat atcatattcc ggtcgaactg
     3301 ctcgcaaaag tccttcagac gctttcgaag agcgggatta ttgaaagcca caacggcccc
     3361 aagggtgggt atctactcgg gaaaaatcct cgggagatca ccatcgctca cgtcctcgaa
     3421 agtctggagg ggccactcgg catcatggat tgctcgcatg agaaagacgg tgatgcctgc
     3481 atgcaacgtg aacactgtaa catccggacc ccgctcctga aaatccagaa cagcatctat
     3541 cagcttttga acaacatgac cttgcaagat atgttgggag gcacccctct catcaccatt
     3601 caatccgtcg cgacggaaca acaaggagtc gagcgatgaa gttccccatc tacctggaca
     3661 accatgcgac caccccgatg gacccgcggg tgctggaaac aatgctgccc tatttcacag
     3721 aaaagttcgg gaacgccgcc agccggaacc acgcgttcgg atgggaggcc gaggaagggg
     3781 tagaaaccgc ccgcaaacag atcgccaagt tgatccatgc ggatgccaaa gaaatcgtgt
     3841 ttacgagtgg cgccacggaa tcaaacaacc tcgccctcaa aggcgtggtg gagatgtacc
     3901 acgagaaggg cgaccatatc atcacgtcgt cgaccgagca tcgtgccgta ctggatacgg
     3961 ccaagacgct cgagaccaag cggggcgtga aggtgactta tctgcctgtc gataagttcg
     4021 gaatggtgaa cccggaggac gttcgtaacg ccatcaccga caaaaccatt ctcatctccg
     4081 tcatgtttgc caataatgaa atcgggacga tcaacccgat caaggaaatc gggaagatcg
     4141 cgaaagaaaa aggcatcctt tttcactgcg atgcaaccca gggcgtaggt aaggtcccca
     4201 tcaatgtgca ggagatgggc atcgatctca tgtcgtttag cgcccacaag atttacgggc
     4261 ccaaaggcat cggcgcgctc tatgtcagaa aaaagaatcc ccgtgtccgc atcgcggctc
     4321 aaatggatgg gggtggacat gaacgtggca tgcgatccgg cacgctacct gtaccgttga
     4381 tcgtgggatt cggaaaggcc tgcgagctct gcgagcagga aatggcggcc gacgccgcgc
     4441 gcctctccgt catgcgtgac cggctgcatg cgaccattac caaggcgctt gaggatgtct
     4501 acttgaatgg ccatccgacg gaacggctcc cgcacaattt gaatatctcc tttgcgtatg
     4561 ttgaagggga atcgttgctg atggggtgca aagaaatcgc gctctcttcc ggctcagcct
     4621 gcacctccgc gacgttggaa ccttcctacg tcttacgagc cctcggtgtg ggagcggaat
     4681 tggcgcattc ttcgatcaga ttcggcctgg ggcgcttcac gctcgacgag gaagttgact
     4741 atgccgcaaa gaagattatc gaaactgtga ccaagctgcg cgaaatgtcg ccgctctatg
     4801 aaatggcgaa agaaggcatc gacctgaaga ccgtgcaatg ggcagcgcat taatttgaaa
     4861 ccaccatctg agtgatattt tccggaggac accatggcct atagcgaaaa agtcgtcgac
     4921 catttcaaca atccccgcaa tatgggcagc ttcaagaagg aagaagacgg ggtgggtacg
     4981 ggcatcgtcg gcgcccccga gtgcggggac gtcatgaagc tccaaatcaa agttcaaaac
     5041 gacacaattg tggacgcgaa gttcaaaacc ttcgggtgtg ggtccgctat cgctagttcc
     5101 agcctggcca cagagtggct gaaaggcaaa acggtggagg aagcgggcaa aatcaagaac
     5161 accgatatcg tgcaggaact caacctgccc ccggtgaaga ttcactgttc ggtcttggct
     5221 gaggatgcca ttaaggcggc ccttgccgac tatcaaaaga aagccgactc gaagtaaccg
     5281 acaggaagga gcgcactatg gatgtcacaa cgaataccga tgcccaagcc cctgtgatta
     5341 cgctgagtga tgcggccctg aaggaagtga agcgcctgat caatgtccaa ggcattga
//
LOCUS       DAOQDS010000130         5313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_9865_length_5313_cov_21.763028, whole genome shotgun sequence.
ACCESSION   DAOQDS010000130 DAOQDS010000000
VERSION     DAOQDS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9865_length_5313_cov_21.763028"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2709
                     /locus_tag="PK589_18255"
     CDS             <1..2709
                     /locus_tag="PK589_18255"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ21609.1"
                     /translation="LIGMSSLDIGIWINPDDRRHLAEALRRDGFLRHLEKEFRTKSGE
                     LRHGLFNVEPVCIGHENCVLTLVLDITRRTEAEAALRRSQTVLQAHQHALMRLTKSQH
                     IGSGDWPSALKELMQTSAEAMAVDRASVWLLDRTGAVLECVELYQRNEARHSRGARLD
                     VAQYPRYFAELLEEQVVDAHDVASDPRTAELLQPYLQTMGIASLLDVPIFFGGRLAGV
                     VCHERLGSARPWSNEDAQFATSIGNLVTLAYESKQRREAESALVTAKEAAEFASRAKS
                     DFLATMSHEIRTPMNAIIGMADLLSETPLSEDQREYVQIFRDAGGNLLSLINDVLDLS
                     KIEAGHLDLDLTDFDLSDLVQRAAELVAVRASEKNLELAYQIQPDVPTSLVGDPNRLR
                     QVLLNLLGNAIKFTDQGEVVLRVECDPHAEGPGSLLFTVRDTGIGIPRDKLAVIFERF
                     TQVDSSMTRPYSGTGLGLTISRRLVERMGGRMWVESEPGRGSTFSFTAKFAAHAQPAS
                     PVPTAQWEQLAGLRTLIIDDNATNRLIVRETLSSWGIPSAEAAGGDEALLELRRALTA
                     GMPYRLAILDVRMPKLSGWQVAETIARSPGLAGLSIIMLTSERRAGDQARAREHGVVR
                     YLTKPFRRSDLFNAMMAVLGKTAPAGNPESEAPSQPKFAGDDQSGLKILLAEDFLDNR
                     RMMEFYFKATPHRVATAANGQIALEMFMHGSYDLVLMDIQMPVMDGYAATRAIRAWEK
                     DQRRRSTPILALTANALQSEVQRSLDAGCTAHLTKPIRKAQLLEAIQLYFQTASSQPP
                     VPAYHEEPAVLHVNVELEALMPGFLEHRRQDLIQMTAALDREDFEVMREIGHGLKGAG
                     GTYGLEAITAYGRSLETAASRKDGPALREILERLRRFLDRLQLIYV"
     gene            2722..4116
                     /locus_tag="PK589_18260"
     CDS             2722..4116
                     /locus_tag="PK589_18260"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ21610.1"
                     /translation="MRVLLVEDHPDVRLLFEQVIGARGHEVTACADGESAWEAYGKRS
                     YELVLLDWELRGGGMDGLQLCRRIRSGPGGDRCVIVMITGHDSPESLRAALQAGVNDY
                     LAKPVGVEFLRLRLTIAEQWVESVRRRFAAEDQAQALQSQLADHGKFHDLIGRSPSMV
                     VLYEEIQQIAAVDATVLIEGETGTGKELVARAIHLSSRRAPHTFLAVNCAGFTDSILG
                     SQLFGHKKGAFTGAIDNQEGVFEAAQGGTIFLDEIGDISPAVQTSLLRVLQEREVTRL
                     GESKPRKVDVRVVVATHHNLVEDVEKGTFRRDLLYRIKVARLQLSPLRERPTDIPLLV
                     HAFLGQFRSVMEKPVLHVSPEALQVLTSYSWPGNVRELKSAVESALIHCKGTVVRPED
                     LPPEIRSPQPQATYVPLQRQDERTRMLGALQQTGGNRSEAARLLGMSRRTFYRRLAEY
                     DVAASAEPGAESFL"
     gene            4438..4722
                     /locus_tag="PK589_18265"
     CDS             4438..4722
                     /locus_tag="PK589_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21611.1"
                     /translation="MVEQQGPSQKEGSMEVEGFLLSNDMDQEVLVTIPPSSVGPFDQP
                     AVKERIDGYWIEMSRLPNRRWSDRTRAVGAVLTYLQGLSAISSGFATHTR"
     gene            4759..5076
                     /locus_tag="PK589_18270"
     CDS             4759..5076
                     /locus_tag="PK589_18270"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNJ21612.1"
                     /translation="MWNDQNLLRSAREDNARDGAAGLSVVPGVSLTAKRRPEDLTPRE
                     QEILQLVWAGLTNRTIAEQLHISIKTAEAHRANMMKKLRVSNIAQLLKTALEEGLLAT
                     HAG"
BASE COUNT         1118 a         1439 c         1652 g         1104 t
ORIGIN      
        1 ctgatcggca tgtcgtcact cgacatcggc atctggatca atccggacga ccgacggcac
       61 ttggcagagg ccttgcggcg ggatggtttt ctccggcatc tggaaaaaga gttccgtacg
      121 aaatccggcg aactccggca cggtctcttc aatgtggaac cggtctgtat cggtcacgaa
      181 aactgtgtgc tgacgctggt gctggacatc accaggcgca ctgaggcaga agcggcattg
      241 cgccgcagcc aaacggtgct ccaagcccac cagcatgcgc tgatgcgtct cacaaaaagc
      301 caacatatcg ggtcggggga ctggccgtcc gcgctgaaag aactgatgca gacttcggcg
      361 gaggccatgg ctgtcgatcg ggccagtgtc tggttattgg atcgcaccgg ggcggtattg
      421 gagtgcgtag agctctatca acgaaacgag gcgcggcact cgcggggggc acgtctggac
      481 gtggcgcagt atccgcgcta ctttgccgag ttgctggagg agcaagtcgt tgatgcgcat
      541 gatgtggcca gcgatcctcg aactgctgaa ttgctgcagc cctatcttca aaccatgggg
      601 attgcctcgt tgttggacgt gccgattttc ttcggtggac gcctggccgg ggttgtgtgc
      661 catgaacgtc tcggctcggc gcgaccgtgg agcaatgaag atgcgcagtt tgccacgtcc
      721 atcgggaatt tggtcacgct ggcctacgag tcgaagcaac ggcgtgaggc agagagcgcc
      781 ctcgttactg cgaaggaggc ggccgaattt gccagtcggg cgaagagcga ctttctagcc
      841 accatgagcc atgagatccg gacgcctatg aatgcgatca tcggcatggc cgatctgctg
      901 tcggaaacgc cgctcagcga agaccaacgg gagtacgtcc agatttttcg ggacgcgggg
      961 ggaaacttgc tgagtttgat caacgatgtg ctcgatctct cgaagatcga agccggtcat
     1021 ctggatttgg atctcacgga cttcgatctc agtgacctgg tacagcgcgc agccgaattg
     1081 gtcgcagtgc gtgccagcga aaaaaatctt gagctggcct atcagatcca accggatgtc
     1141 ccgacctcac tcgtagggga tccgaatcgc ctccgccagg tactgctgaa cctcttgggc
     1201 aatgcgatca agttcacaga tcagggagaa gtcgtgttgc gcgtcgaatg cgacccgcac
     1261 gcagaggggc ccggcagtct gttgttcacc gttcgggata ccggcattgg aattcctcga
     1321 gacaagttgg ccgtcatctt tgaacggttc acccaggtgg attcttcgat gacgcggccg
     1381 tatagcggca ctggtctcgg tctcacgatt tcacggcgcc tcgtggagcg aatgggcggc
     1441 agaatgtggg tggagagtga acccggccgc ggcagcacct tcagctttac ggccaagttt
     1501 gctgcgcacg cgcaaccggc ctcaccggtc cccacggcac aatgggagca acttgcgggg
     1561 ttgcggacct taatcatcga cgataatgcc accaatcggc tgattgtgcg ggaaaccttg
     1621 agtagctggg ggatcccttc ggcggaagcg gccgggggag acgaggcatt gctggaattg
     1681 cgccgggcac tcacggccgg catgccttac cgtttggcaa ttctcgacgt gcgcatgccg
     1741 aaactcagtg gatggcaggt ggccgaaacc atcgcacgct cgcccggtct ggccggccta
     1801 tcaattatca tgctgacatc agaacggcgc gccggtgacc aggcccgagc ccgtgaacat
     1861 ggagtcgtgc ggtacctcac gaagcctttt cgacgctccg atctgttcaa cgcgatgatg
     1921 gccgtgttgg gcaagacagc gccagccggg aatcctgaat ccgaagctcc ttctcagccg
     1981 aaattcgctg gagatgacca gtcaggcctg aagattctct tggcggaaga tttcctcgac
     2041 aaccgccgca tgatggagtt ttatttcaag gcaacgccgc atcgggtggc gacggcggcc
     2101 aatggtcaga tcgcgctcga gatgttcatg cacggatcgt atgatctggt cctgatggat
     2161 attcagatgc ctgtgatgga cgggtatgcc gcgacgaggg ccatccgcgc atgggaaaaa
     2221 gaccaacgcc gcagatccac gccgatcctg gcattgacgg ccaatgcgct gcagagtgag
     2281 gtgcagcgga gtcttgatgc gggatgtacg gcgcacctca cgaaacccat tcgtaaggct
     2341 cagcttctag aggcgattca actctatttc cagacagcct cctcccagcc gccagttccc
     2401 gcataccacg aagaacctgc cgtgcttcat gtcaacgtcg agctggaagc cctcatgcct
     2461 gggtttctag agcatcggcg ccaggacttg atccagatga cggcggcatt ggatcgtgag
     2521 gattttgagg tgatgcggga aatcggccac ggtctcaagg gagccggcgg aacatatggg
     2581 cttgaagcga ttacagcgta tggacggtcc ttggaaacgg cggcctcgag aaaagatggt
     2641 cccgcgttac gcgaaatcct ggaacggctc cgccggtttc tggatcggtt gcaactgatc
     2701 tacgtgtaaa ggatagacgg tatgcgcgta ttgctggttg aagatcatcc cgatgtgcga
     2761 ctcctgttcg agcaggtcat cggggcccga ggacatgagg tgacggcctg cgctgatggc
     2821 gaatcggcat gggaggccta cggcaagcgg tcatatgaac tcgtgctgct cgattgggaa
     2881 ttgcgtggtg gaggaatgga cggattgcag ctctgccgga ggattcgctc cggtcccggc
     2941 ggagatcgct gtgtcatcgt catgatcacc ggacacgatt ccccggaatc cttgcgtgcg
     3001 gcgctgcagg ccggcgtcaa cgactattta gccaagcccg ttggtgtcga gttcttacga
     3061 ttgcgattga cgattgccga acagtgggtc gaaagtgtgc ggcgccgctt tgcagctgaa
     3121 gatcaggctc aggcgctgca atcgcaactg gcggaccacg gcaagtttca tgatctgatc
     3181 ggccgaagtc cgtctatggt ggtcttgtac gaggagattc aacaaattgc ggcagtggat
     3241 gcgaccgtgt tgatcgaagg cgaaaccggc acgggcaagg aactggtggc ccgggcgatc
     3301 catctgtcca gccggcgcgc gccgcacaca tttcttgccg tcaattgcgc ggggttcacc
     3361 gactcgattt tgggcagtca gttattcggt cacaaaaagg gcgcatttac cggcgctatc
     3421 gacaaccagg aaggtgtctt tgaggccgcg caagggggca ccatttttct ggacgagatc
     3481 ggcgatattt cccctgcagt tcagacgagc ctgctgcgcg tgcttcagga gcgggaagtc
     3541 acgagactgg gagaatccaa gcctcgaaaa gtcgatgtcc gtgtcgtggt cgcgacccat
     3601 cacaaccttg tggaagacgt cgagaaaggc acgttccgga gggatctgct ctatcgcatc
     3661 aaggtggctc gccttcagct gagtccgctc cgtgaacggc cgacggatat tccgctcttg
     3721 gtccatgcgt ttcttggtca atttcgttcg gtgatggaaa aaccggtgct gcatgtgagt
     3781 ccagaggcgc tgcaggtctt aacgagctat tcgtggcccg ggaatgtgcg ggagttgaag
     3841 agcgccgtcg agtcggcctt gattcattgc aaagggaccg tcgtgcgtcc ggaagatctc
     3901 cccccggaga tccggagccc gcaaccacag gccacctatg ttccgctcca gcgacaagac
     3961 gaacgaaccc gcatgctggg agccttgcag cagacgggcg gcaatcgttc agaggcggct
     4021 cggttactgg ggatgagccg gagaaccttt tatcgtcgcc tggctgaata cgatgtggct
     4081 gcctcggcgg agcccggcgc agagtccttc ctgtgaactg gtgagcgtat gcgcacgcgc
     4141 gcttcgttgc tctcaagttg ccctcaattg gacgtatttc ttaataacgt catgcaccgt
     4201 tcacgctgat ctcctttcgg tttccctatt gcaagtctgc gtgttccgca catcactgtg
     4261 catagagtca catgacacac gtgtgacaca gcacagtgtg ccatctcatt gacgcatgcg
     4321 ggtgttcacc gatgtggttt cgggcctaag cccccagtat ttcgtggagc ttgttgttcc
     4381 ttcatcgatg gcacgatcct tgatacatga tctccacgaa cagcgtagtg gagagaaatg
     4441 gtggaacagc aagggccgag tcagaaagag ggcagcatgg aggttgaagg atttctcctg
     4501 tcgaacgata tggaccagga ggtcttggtg accattccgc cttcatctgt cggcccattc
     4561 gatcaaccag ccgtcaaaga gcgtattgat ggatattgga tcgagatgtc tcgattgccg
     4621 aacaggagat ggtctgaccg gacgcgtgcg gtcggagccg tgctgacgta cttgcaaggc
     4681 ttgtcggcaa tttcgtcagg gtttgctacc cacacgagat gacgagaagc tgcaaccaga
     4741 accaaaggag tacagaccat gtggaatgac cagaatctat tgcggagtgc ccgggaagac
     4801 aatgcgcggg atggagcagc cggactgagt gtggtgccgg gcgtgagtct cacggcgaaa
     4861 cgtcgccctg aagatttgac cccgcgcgaa caggaaattc tgcagctggt ctgggcggga
     4921 ttgaccaatc gcacgattgc ggaacaattg cacatcagta tcaagacggc cgaggcgcat
     4981 cgggccaata tgatgaaaaa actccgggtc tccaatattg cgcagctatt gaaaacggcg
     5041 ctggaagagg ggctgctcgc cacccacgcg ggctgaaggg attcgcggcg gcctgcactc
     5101 tcgggggaag tcccacttcc ggcagtgcat cggctcccgc gagtccgccc cctcacagaa
     5161 caaatgaggc tcgcaccggc gacggcgttt gcagtagaat gccgcaccgt cgctggagtg
     5221 agccgtggtc gaacaacgca tcgaagaaat tacccgcgcc aatctcgcct tttatgccgc
     5281 ctttgaaagt ctggatatgc tccagatgga caa
//
LOCUS       DAOQDS010000131         5311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_9875_length_5311_cov_21.193874, whole genome shotgun sequence.
ACCESSION   DAOQDS010000131 DAOQDS010000000
VERSION     DAOQDS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9875_length_5311_cov_21.193874"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..876)
                     /gene="fabG"
                     /locus_tag="PK589_18275"
     CDS             complement(133..876)
                     /gene="fabG"
                     /locus_tag="PK589_18275"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNJ21613.1"
                     /translation="MSLQGKVAIVTGGAQGIGRAIAEGLAEDGADIAVADLDPARSQD
                     AVAAIQKLGRRALSVKVNVSDWNDAKAMADQVMQEWGRIDILVNNAGITRDGLLLRMK
                     EEDWNLVLQVNLNGTFHCTKAVLLPMTKQRFGRIVNIASIVGAMGNVGQANYSASKAA
                     VIGFTKTAAREYASRAVTVNAVAPGFIDTAMTQGLPPEVKEALQKQIPLGRLGLPSDI
                     AAAVRFLVSDAASYITGQVLHVNGGMLMV"
     gene            complement(907..1860)
                     /gene="fabD"
                     /locus_tag="PK589_18280"
     CDS             complement(907..1860)
                     /gene="fabD"
                     /locus_tag="PK589_18280"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNJ21614.1"
                     /translation="MTASAIGFLFPGQGSQSVGMGRGLYDAFPAVRAVYEEASTILGY
                     DAAQLCFEGPVERLNLTEHTQPALLVSSAAALKALEPAGLSPLAVAGHSLGEYSAVFA
                     AGGLSFRDAVALVQKRGRYMAEAVPPGTGLVAALLGLSAEVVKAVCQEASSVGVVAAA
                     NFNSPGQVVIAGEKVAVERAIDIAKSKGCKKAIPLPVSVPVHTPLMQGAADRLAAEFG
                     GVAWRDLTVPLINNAEAVALQRSEDIRASLVRQLPSSVRWEESVQTMAKMGVTTFLEI
                     GPGTVLTGLVKRILPGAVTANVHDQKSLDAALTTVGVNRQP"
     gene            complement(1967..2944)
                     /locus_tag="PK589_18285"
     CDS             complement(1967..2944)
                     /locus_tag="PK589_18285"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNJ21615.1"
                     /translation="MIRGRITGTGSYAPERVMTNSDLEQLVATSDEWIRERTGIRERR
                     IAAPGQACSDLALIAAERALKAAGLSGSDLDLILLATCTGDMPLPSTACLLQHRLGAT
                     RAAACDISAACCGFVYALGVADAYVRTGMRHVLVVGSEVMSTITDWTDRNTCILFGDG
                     AGAAVVSAAEGERGILSSHLHSDGSLSDLIVVPGGGTRIPPSEAMVGERSQYIKMKGN
                     ETFKVAVRTLEEVARETLAAHQLSVDDLDLYIPHQANLRIIKAVADRLGLPLEKVVLN
                     MDRYGNTSAASIPIALDEAVRDGRVKDGQLVMFGAFGAGLTWASTLIRW"
     gene            complement(2941..3972)
                     /gene="plsX"
                     /locus_tag="PK589_18290"
     CDS             complement(2941..3972)
                     /gene="plsX"
                     /locus_tag="PK589_18290"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNJ21616.1"
                     /translation="MKIAVDAMGGDHGPAPVIEGAMQAAQELGVGIILVGKEDELTAA
                     CRKLNCNDTRITIRHAPQVVEMHESPAAVARKKRDSSIWVATELVKSGEADAVVSPGN
                     TGASMVASFFVLGLIKGVERPAIATMLPTLTGSAIMLDVGANVDCSAQHLEQFALMGN
                     EFARHVYAKPNPRVGLLSIGEEDSKGNEVTKEAFKLLKTSPLNFIGNIEGREVYSGSA
                     DVVVCDGFIGNVALKISEGVADVIKKLLMKELSGSWLGRLAYPLIAKPLLNLKRKIDY
                     AEFGGAPLLGVNGTTMICHGRSSAKAIKNAIRRAKGLAETRLDELIQRDIEESLRRHQ
                     DERLEDKQA"
     gene            complement(4124..4309)
                     /gene="rpmF"
                     /locus_tag="PK589_18295"
     CDS             complement(4124..4309)
                     /gene="rpmF"
                     /locus_tag="PK589_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNJ21617.1"
                     /translation="MPNPKHKHSRSRRDMRRTAKTRLVPPGFSLCPQCHELKLPHYTC
                     MNCGTYKGKAVIVVEES"
     gene            complement(4348..5019)
                     /locus_tag="PK589_18300"
     CDS             complement(4348..5019)
                     /locus_tag="PK589_18300"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNJ21618.1"
                     /translation="MSVESRGPAACLLIDTGEDRLVMTVPMDMLNPAIVDIGPEGLPL
                     SCSASGPQLGLDEPEDKFGGPLAIQLELSQQEGRISVTGTLEGTAIRQCVRCLSEYAD
                     PLLVSLYAEYLPQASVAAKSAPAEQGRRNTRRGAQPVEPEEEADEEDELYWYQGDHLD
                     LVPMLREQVILAAPMQPICREDCPGLCPQCGQNLNERRCDCPPAQVPSPFRVLRGRGP
                     KGENA"
     gene            complement(5037..5294)
                     /gene="rpsR"
                     /locus_tag="PK589_18305"
     CDS             complement(5037..5294)
                     /gene="rpsR"
                     /locus_tag="PK589_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNJ21619.1"
                     /translation="MERGDRGNSGGGGGRFFQRRKPCRFCVDKAPIDFKDVGLLRNFL
                     TERGRIVPRRISGNCLQHQRVLTVAVKRARQIALVSFAEER"
BASE COUNT         1114 a         1762 c         1347 g         1088 t
ORIGIN      
        1 ataattttct tcacccgttc atctactgtt gccatggact tgcccacctc cttcctatct
       61 tatctcatcc gccacatttt ttgccggaac atccggttcg accgaaccga tcgagacaca
      121 ggcccgactc acttatacca tgagcatgcc gccgttcacg tgcaacacct gccccgtgat
      181 atacgacgct gcatccgaca ccaaaaaccg aaccgccgcg gcaatatcgg aaggaagccc
      241 caatcgtccc agggggatct gtttctgcaa ggcttctttc acctccggcg gcaacccctg
      301 cgtcatagcc gtatcgataa agcccggcgc cacggcgttg accgtcacgg cgcgactggc
      361 atattcacgc gcggccgttt ttgtaaaccc gatgaccgct gcttttgacg ccgaatagtt
      421 cgcctgcccc acgtttccca tcgccccgac gatcgaggcg atattgacga tacggccaaa
      481 ccgttgcttc gtcatgggga gcaacaccgc tttggtgcaa tggaatgtcc cgttcaaatt
      541 gacctgcagg accagattcc agtcttcttc cttcatccga agcagcaacc cgtcgcgcgt
      601 aatcccggcg ttgttcacca ggatatcgat ccgaccccat tcctgcatga cctgatcggc
      661 catggccttc gcatcattcc agtcggacac attcactttg acactgagag cccgtcgccc
      721 cagtttctga atcgccgcaa cggcatcctg cgagcgcgcc gggtcgaggt ctgccacggc
      781 gatatccgcg ccatcctcag ccaacccttc cgcaatcgca cgcccgattc cctgggcccc
      841 gccggtgaca atcgctactt ttccctgtaa tgacatcagt ctactcccac gcaatctcga
      901 agctgcttat ggctgcctgt ttacgcccac agtggtgagg gctgcgtcca atgacttttg
      961 atcatggaca ttggcggtga ctgcacccgg aagtatccgc ttgaccaagc ccgtcaagac
     1021 cgtcccgggc ccgatttcaa gaaaggtcgt cacacccatc ttggccatgg tctgtaccga
     1081 ctcttcccat cgcacggacg agggcaactg tcgcaccaac gatgcccgga tgtcttctga
     1141 tcgctgcagg gccaccgcct cggcattatt gatcaatggc accgtcaaat cccgccacgc
     1201 gactccgccg aattccgcgg ccagccggtc tgcagccccc tgcatcaagg gtgtgtgaac
     1261 cggtacactc accggaagcg gaatcgcctt cttgcatccc ttcgatttgg caatgtcgat
     1321 cgctcgttcg accgcaacct tctcgcccgc aatgaccacc tggccgggag aattgaaatt
     1381 cgccgcggcg accacaccga cagaggaggc ctcctgacag acggctttca ccacttccgc
     1441 agaaagaccg agcaatgcgg ccaccagccc tgtccccggc ggaacagctt cggccatata
     1501 ccgcccacgc ttttgcacca gagccaccgc atctcgaaac gatagcccgc cggctgcaaa
     1561 gactgctgaa tattccccga gactatgccc ggcaaccgcc agcggactca gtccggccgg
     1621 ttccaaggcc ttcagcgctg ctgcactgct gacgagcaag gccggttgcg tgtgttctgt
     1681 gagattgagc cgttcaaccg gcccctcaaa acagagctga gccgcatcgt agccgaggat
     1741 ggtggaggcc tcttcataca ccgccctcac cgccgggaac gcatcataca acccgcgtcc
     1801 catgcctact gattgcgacc cctgtccggg gaacaaaaat ccgatggctg aagctgtcat
     1861 aaccgcgtaa ggtatcgtag aaaccaggct caatgtcaac ggaaaaagcc ggacacattc
     1921 ggcaccgccg gacttcacat ccggctaccg accagtggcc gcaccttcac cagcgaatca
     1981 gcgtcgacgc ccaggtcagc cccgcgccga aagctccgaa catcaccaat tgtccatcct
     2041 tgacccgtcc gtcccgcacg gcttcgtcca gggcgatcgg gatggaggct gccgacgtgt
     2101 tgccgtatcg atccatattc aagacgactt tttcgagcgg aagaccgaga cgatcggcca
     2161 ccgctttgat aatgcgcagg ttcgcctgat gaggaatata cagatcaaga tcatcgactg
     2221 acagctggtg cgctgcgagc gtttcgcgtg cgacttcctc cagagtccgt actgcgacct
     2281 tgaacgtttc gttgcccttc atcttaatgt actgcgaccg ctcgcccacc atcgcttcag
     2341 aaggaggaat ccgggttccg cccccgggca ccacgattag atcggacaaa ctgccgtccg
     2401 aatgcaggtg gctggagagg atgccacgtt ctccctcggc agcgctcacc accgcagcgc
     2461 cggcgccatc gccgaataaa atacaggtat tgcgatcggt ccaatccgta atcgtggaca
     2521 tcacttccga gcccacgacg aggacatggc gcatccccgt tcggacatag gcatcggcga
     2581 cgcccaacgc atagacgaac ccgcagcaag cggccgagat gtcgcaggct gccgctcgcg
     2641 tcgcacccag gcggtgctga agcagacagg cagtcgacgg caacggcatg tcgcccgtgc
     2701 aggtggccag gagaatcagg tcgagatcgc tcccggaaag tccggcagct tttaatgcgc
     2761 gttccgcggc gatcaacgct aaatctgaac aggcctgtcc cggagccgcg atacgccgtt
     2821 cccggattcc ggtgcgctcg cgaatccatt catccgaggt cgccaccagc tgctccaggt
     2881 cactattggt catcacccgc tcgggcgcat aagacccagt tcctgtgatc cgcccccgta
     2941 tcacgcctgt ttgtcctcca gccgctcgtc ctgatgccgt ctcaagctct cctcgatgtc
     3001 gcgttgaatc agctcatcta accgggtttc ggccaagccc ttcgctcgtc gaatggcatt
     3061 tttgatggcc ttggcggatg agcggccatg gcagatcatg gtcgtcccat tcacccccag
     3121 caacggcgcg ccgccgaatt ccgcatagtc gatttttcgc ttcaaattca gcaacggctt
     3181 tgcaatcaac ggataagcca accgcccgag ccaggaaccg gagagttcct tcatcaacaa
     3241 cttcttgatg acgtccgcga cgccttccga aatcttgagg gccacattgc cgataaaccc
     3301 gtcgcatacc accacatcgg cgcttccgct gtagacttcg cgtccctcga tattcccgat
     3361 aaaattcaat gggctggttt tcaacagctt gaacgcttct ttcgtaactt cgttcccctt
     3421 gctgtcttct tcgccgatgc tcaacagtcc tacgcgcgga ttgggtttgg catagacatg
     3481 cctggcgaat tcattgccca tcaaggcgaa ttgctccaga tgttgcgccg aacaatcgac
     3541 attggccccc acatcaagca tgatcgcact tcccgtcaat gtcggtaaca tcgtggcaat
     3601 cgccggccgc tcgacacctt tgatcaatcc caacacgaag aatgaggcca ccatgctcgc
     3661 gccggtattg cctggactga ccacggcatc ggcttcaccg gatttgacca gctccgtcgc
     3721 cacccaaatg gacgagtccc gcttcttcct ggccacagcg gcaggtgact cgtgcatctc
     3781 caccacttgc ggcgcgtgcc gaatggtaat ccgcgtatcg ttacagttga gtttgcggca
     3841 tgcggcggtc agctcgtctt ccttgccgac caaaatgatg ccgacaccca gttcctgcgc
     3901 ggcttgcatc gcgccttcaa tgaccggggc cggtccgtgg tctccgccca tcgcatcgac
     3961 ggcaatcttc atgtgcggtt agcggtccac ggatagagaa gtgaagagtc gtgcagagac
     4021 gccctgccgg ctaggacctg aacggcctca cgcatgacat agcgtcggat cgtgatcacg
     4081 gtgggcaatt gtacccgaat cacgtcgaaa aaataaaacg cgactaggat tcctcgacga
     4141 cgatgacggc cttgcctttg tacgttccgc aattcatgca cgtatagtgc gggagcttca
     4201 attcgtggca ctgcgggcag agcgaaaatc ctggaggcac cagccgggtc ttcgctgttc
     4261 gccgcatgtc gcgacgggat cgtgaatgtt tgtgttttgg atttggcatg acgactcctt
     4321 caacgggcac aacacccggt taacagacta tgcgttttca ccttttggcc cacgccctcg
     4381 cagcacccga aacggactcg gcacttgcgc cggagggcag tcgcatcggc gctcattaag
     4441 gttctgtccg cactggggac aaagtcccgg gcaatcctcg cgacatatcg gctgcatcgg
     4501 agcggccaga attacctgtt cgcgcagcat cgggacgaga tcaagatgat cgccctgata
     4561 ccagtacagt tcatcctctt catccgcctc ttcttccggt tcaaccggtt gagcaccgcg
     4621 tctcgtattc cgtcttccct gttccgccgg agcagatttc gcagccacac tcgcctgcgg
     4681 aagatattcc gcatagagag agacgaggag cggatcggca tattccgaaa gacaacgcac
     4741 gcattgacgg atcgcggtgc cttccaacgt gccggtgacc gagatccgtc cttcttgctg
     4801 cgagagctca agctgaatgg ccagcgggcc gccaaactta tcttccggct catccaaccc
     4861 cagctgaggc cccgaagccg agcatgacag gggcagccct tccggaccaa tatcgacaat
     4921 cgctgggttg agcatatcca tgggcaccgt catgacgaga cgatcctccc ccgtatcgat
     4981 caggagacag gctgccgggc cacgtgactc aacactcacc aaaacctatc cctgccctat
     5041 cgctcttcgg caaaactcac cagggcgatc tggcgagccc gcttcactgc gaccgtcaac
     5101 acccgctgat gctgcaagca attgccggaa atgcgacggg gaacgatacg tccacgctcg
     5161 gtcaaaaaat tccgcaacag gccaacatcc ttgaaatcaa tcggtgcctt atccacacaa
     5221 aaccggcaag gcttgcggcg ttgaaaaaac cggcctccgc cccctccgct gtttccacga
     5281 tctcctcgtt ccatgatcgc ctccgtctcc t
//
LOCUS       DAOQDS010000132         5281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_9981_length_5281_cov_22.452545, whole genome shotgun sequence.
ACCESSION   DAOQDS010000132 DAOQDS010000000
VERSION     DAOQDS010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9981_length_5281_cov_22.452545"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1167
                     /gene="arsS"
                     /locus_tag="PK589_18310"
     CDS             130..1167
                     /gene="arsS"
                     /locus_tag="PK589_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250199.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNJ21620.1"
                     /translation="MALTLVGQHKPLASSAEQLKVLAEIGSCLPFADQLRRSGLSPLH
                     ATGITVLQINVGKLCNQTCRHCHVDAGPDRTERMSRETAEQCIAALAMTDIPTVDITG
                     GAPELNPYFRWLVEQARALNRHVMDRCNLSVLLLPSQADLATFLAAHQVEIVASLPYY
                     RAAQTDAQRGEGIFEKSIEALQLLNSLGYGREGSGLIVNLVHNPVGAFLPAKQEAIEA
                     QFRKELRAKHGVEFNHLYTITNMPVSRFLEFLVESGNYLGYMERLANAYNPAAAGSVM
                     CRYTLSVGWDGMLYDCDFNQMLDLPVQPDAPAHIRDFAPARLHHRQITTRNHCYGCTA
                     GAGSSCGGTVT"
     gene            1308..2441
                     /locus_tag="PK589_18315"
     CDS             1308..2441
                     /locus_tag="PK589_18315"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNJ21621.1"
                     /translation="MDMSVLTFALVLALAYANGSNDVSKAIATLVGSGVTNYRTAIMW
                     GTAWTVAGAGFATLVAGAMVKTFSNGLLQTGTMLSPSIALAILFGAMLWVLVASRTGL
                     PVSTTHALTGAIVGTGLVAFAGEGLLWAAIGKKIALPLLLSPFLAFGLSLLLHPVMRR
                     AATKWEGSCLCLMPASRALFTVDARGSTRTVFQSTVFGQPVVAVPVQCDRAGLRGLTV
                     GLDTVHWLSSGMASFARGTNDAPKIVAMLLLGSTTATWPSVWFQFAAFGGVAFAMGLG
                     SYLGGLRVTEMLAEKVTRMEHAEGLSANLTTSTLVLCSGWLGLPVSTTHVSSSAIIGI
                     GLLKGFNAVRWTTVRDMVLAWVITLPAAGCFAALAYVLLANFA"
     gene            complement(2588..3307)
                     /locus_tag="PK589_18320"
     CDS             complement(2588..3307)
                     /locus_tag="PK589_18320"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNJ21622.1"
                     /translation="MNVDPITSNLYSDQECQGVPADAVAASLGCGNPTALAELQDGET
                     VLDLGSGGGIDVLLSANRVGPKGKAYGLDMTDEMLALARENQRTAGVENVEFLKGEIE
                     NIPLPDGSVDVIISNCVVNLSGEKERVLAEAFRVLRPGGRLALSDIVVRGAVPSEIRK
                     NLELWAGCVAGALEETQYGDLLRQAGFTEIGIEPTRIYQAGDVKELLIGGDLSSDQLV
                     AEVDGKFMSAFIRATKPVSKS"
     gene            3776..4813
                     /gene="hflK"
                     /locus_tag="PK589_18325"
     CDS             3776..4813
                     /gene="hflK"
                     /locus_tag="PK589_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNJ21623.1"
                     /translation="MVWDPKDPWSKKGDDLDQAFKQAQGQLRNLLPTGGFRNLLLVAF
                     TVFLIWQSAFIVAPDEEGVVKRFGIPVRVVEPGPHMKIPVVESVLQPKVAKLHRVEIG
                     FRTDRQGRQQMIPQEALMLTGDMNILAIEFIVQYKIKSSREYLFNVADIDDTIGKAAE
                     ASMREVIGKSKIDEALTTGKAQIQHDTQELLQHILDEYKTGVQVAAVQLQDVDPPEAV
                     AAAFKDVTNAKEDREKLINQAQGYRNDITPKAKGEAAQLVNQAKGYAQARLNRAQGET
                     NRFLATLKEYNQSKDIISKRIYIETLEDVLPHIDKFVLDGKGGERALPYLPLDRFSKP
                     APTAATQERTP"
     gene            4810..>5281
                     /locus_tag="PK589_18330"
     CDS             4810..>5281
                     /locus_tag="PK589_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNJ21624.1"
                     /translation="MSKQGFAVAFVGIVIGLLILGASPFYIVDVTQNAIVVQLGKPVR
                     NVTEGGLYLKVPFIEEVTYFDKRLLDYDSNAQDVITQDKKTLLLDNFAKWRITDPLKV
                     YQAFQSQRGALQRLHDIIYSELRVELGRHELAEIVSSARAQLMAVVTQRANEKAS"
BASE COUNT         1113 a         1637 c         1417 g         1114 t
ORIGIN      
        1 gagcaggtac tggacttcgg atgttgcgaa ttgcgatttc ccacctcgac cttactcacg
       61 ctgcagcaaa aacattgaaa ccggaatgca ggcgatgaac atactccata gtacttggaa
      121 taactctcca tggcactgac actcgtcggc caacataaac cgctcgcttc ctctgccgag
      181 cagctgaagg tactggcaga gatcgggtcc tgcttgccat ttgcggacca gcttcggcga
      241 agcggcctgt cgccgctcca cgcgaccggc atcacggtgc tccaaatcaa cgtgggaaaa
      301 ctttgcaatc aaacctgccg ccattgtcac gtagacgcgg ggcccgaccg aacggaacgc
      361 atgtcccggg agacagccga gcagtgcatc gcggccttgg ccatgaccga cattcctacc
      421 gtcgacatca caggtggcgc accggaactg aacccctatt tccgctggtt ggtcgagcag
      481 gcccgtgccc tgaatcgcca tgtcatggat cgctgcaatc tgtcggtgct gctgttgccc
      541 tcgcaggcgg atctggcgac ctttctcgcg gcgcaccagg tggagattgt cgcgtcactg
      601 ccgtattacc gtgccgcgca aactgacgcg caacgcggcg aaggcatttt cgagaagtcc
      661 attgaagctc tgcagctcct caatagcctc ggctacggac gcgaaggcag cgggttgatc
      721 gtgaatctcg tgcacaatcc ggtcggcgcc ttcctgcccg ccaaacagga ggccatcgaa
      781 gcgcaattcc ggaaggagct ccgcgccaag catggcgtgg aattcaacca cctctatacc
      841 atcaccaaca tgccggtcag ccgcttcctc gaattcctgg tggagagcgg gaactatctg
      901 gggtacatgg aacggcttgc gaatgcgtac aatcccgccg ccgccggcag cgtgatgtgc
      961 cgctatacac tctcggtcgg ctgggacggc atgctctacg attgcgactt caaccagatg
     1021 ctggaccttc cggtgcagcc cgatgcccct gcgcacatcc gggactttgc ccctgcccga
     1081 cttcatcacc ggcagattac cactcgcaat cattgttacg gctgcacggc cggtgcagga
     1141 tcgtcgtgcg gtgggacggt gacctaatca gaaatgctga acgcaccgcc caacttcgcg
     1201 ctcagtcatt cttccccttc acgatacttg accggtatgc ctcggcggac tcacatccct
     1261 acggccttgt tggaccgacg gcttgagcag tctgcgcagg agatcacatg gatatgtcgg
     1321 ttctgacttt tgcgctggtg ttggcgttgg cctatgccaa tgggagcaat gacgtctcga
     1381 aagccatcgc cacgctggtc ggcagcggcg tcacaaacta ccgcacggcg atcatgtggg
     1441 gaacggcctg gaccgtggcc ggagccggat tcgccaccct ggtcgccgga gcgatggtga
     1501 agaccttcag caacggtcta ttgcaaaccg gcaccatgct ttcaccctcc atcgccctcg
     1561 cgattctgtt tggtgcaatg ctctgggtgc tcgtggcatc aagaacggga ctgcccgttt
     1621 cgacaaccca tgcgctcacc ggcgccatcg tgggaaccgg tctggtggcg tttgcgggtg
     1681 agggactatt gtgggccgcc atcggcaaaa aaatcgcgtt gcccttgctg ctcagtccct
     1741 ttctggcctt tggtctctcc ctcctcctac atcctgtcat gcgacgagcg gcgacaaaat
     1801 gggaaggatc ctgcctgtgc ctcatgccgg cctcgcgggc actctttacc gttgacgcac
     1861 ggggcagcac acgaacggtg tttcagagca cggtgtttgg ccagccggtc gtggcggtgc
     1921 cggtgcaatg tgaccgcgca gggctgcgag gcctgacggt gggcctcgat acggtccatt
     1981 ggctgtccag cgggatggcc tcctttgccc gcggcaccaa cgacgcaccc aaaattgtcg
     2041 cgatgctcct gctcggcagc acgacagcca cgtggcccag cgtctggttt cagttcgcgg
     2101 ccttcggcgg cgtggcattc gccatgggtt tagggagcta cttgggcggg ctccgagtca
     2161 ccgagatgtt agccgaaaag gtcacgcgta tggaacatgc agaaggattg tctgcgaacc
     2221 tcactacctc cacgctcgtc ctctgttccg gctggttagg attgccggtg tcgaccacac
     2281 acgtcagcag ttccgccatt atcgggatcg gtctcctcaa aggcttcaac gctgtccgct
     2341 ggacaaccgt gcgggacatg gtgttggcct gggtgatcac tttgccggcc gctggatgtt
     2401 tcgccgctct ggcctacgta ctccttgcca acttcgctta gccgggcttc tgcaccagga
     2461 aaccgctgcc aaccccagag agtgaagtat tgttcacaaa acgacagccg cttcgtgtct
     2521 cccgttggcg cgaggactgt ttgcacaagc tctatgcgac tgctcctttg tggtccctgc
     2581 tcgcgcccta gctcttactt accggtttcg tagcccggat gaatgcactc atgaacttgc
     2641 cgtccacttc agccacaagt tggtcactgg agagatctcc gccgatcaac aactccttca
     2701 cgtctccggc ctggtagatg cgcgtcggtt cgattccgat ctcggtaaaa cccgcctgcc
     2761 gcaacaaatc cccatactgc gtctcttcca gcgcccccgc cacgcaacca gcccacaatt
     2821 ccagattctt tcggatctcc gatggtaccg cccctcgcac gacaatatcg gacaacgcca
     2881 agcgccctcc cggtcgcaga acgcgaaacg cttccgccaa cacacgttct ttttccccgg
     2941 acaaattcac cacgcagttg ctgatgatca catccaccga cccgtcgggc agcgggatgt
     3001 tttcgatttc gcccttcaga aactcgacat tctccacacc ggccgtacgc tgattttctc
     3061 tggccaacgc cagcatctcg tcagtcatat caagtccata cgccttgcct ttcggtccga
     3121 ctcggttggc ggagagcaac acatcgattc ccccgcctga tccgaggtcc aacacagttt
     3181 cgccgtcctg aagttccgcc agtgccgtgg gattcccgca accgagcgac gctgcaacgg
     3241 catctgccgg taccccctgg cactcctgat ctgaatacag gttggatgta atcgggtcga
     3301 cattcattaa cgccggggca gcccgcagca ggaactgcct ccggttttcg cccgtgccgc
     3361 tgcttcacca tacttttcct tgattaactc tcgaatcgct tgggcatcca tttcggcatc
     3421 tcctctttgc atcacactta ttgatatatc agacacactt acactcaaca tcgcaatgga
     3481 acgagagctg tgaccgcccg gcctcaaggc aatctatcag ctcacagacg caccgttcgc
     3541 cacccttcag ttcgtccaaa tttcaagtct ggtctggcca tgccagcgca tgaaacagtt
     3601 ccaccgtccg gaccggattc atgatcacgg tgtcttgcca ccactctaca tcaatacatt
     3661 ttgatgagta aaacagctgg ctgttgtcgc cgttataggt gttgctcaca ggtgctcact
     3721 ttgctatggt gacccgcgag catcgttgcc accacttaca gaattgagcc acgcgatggt
     3781 ctgggacccc aaagaccctt ggagcaaaaa gggcgacgat ttggatcagg ccttcaagca
     3841 ggctcaaggc caactccgca acctgctgcc caccggcgga tttcgcaatc tcctcctggt
     3901 cgccttcacg gtcttcctca tctggcaaag cgcgttcatc gtggcccccg atgaagaagg
     3961 ggtcgtcaaa cgcttcggca ttccggttcg agtggtcgaa ccgggaccac acatgaaaat
     4021 tccggtggtc gaaagcgtgc tgcagcccaa agtggcgaaa ttgcaccgcg tggaaattgg
     4081 atttcgtacc gaccggcagg gccgtcagca gatgattccg caggaagcct tgatgctgac
     4141 cggggatatg aacattctgg ccatcgaatt cattgtccaa tataagatca aaagttcacg
     4201 cgagtatctg ttcaatgttg ccgacatcga cgatacgatc ggcaaggcgg ctgaagcgtc
     4261 catgcgtgag gtcatcggga agagtaaaat cgacgaagcc ctgaccaccg gcaaagccca
     4321 gatccagcat gacacccagg agctgctcca gcacattctg gatgaataca aaaccggcgt
     4381 acaggtggcc gccgtccagt tgcaggatgt cgatcccccg gaagccgtgg ccgcggcgtt
     4441 taaggacgtg accaatgcca aggaagatcg tgaaaaactg atcaaccagg cgcagggcta
     4501 tcgaaacgac atcactccca aagccaaggg agaggcagcc caattggtga atcaagccaa
     4561 ggggtacgcg caggcgagat tgaatcgcgc ccagggtgaa accaatcgtt tcctcgccac
     4621 cctcaaagaa tataaccagt cgaaagacat catcagcaag cgcatctaca tcgagacgct
     4681 tgaagacgtg ctgccccaca tcgacaaatt cgtcctggat ggcaaaggtg gagagcgagc
     4741 cctgccgtac cttccgttag accgtttcag caaaccggct cccaccgctg cgacgcagga
     4801 gcgtacgcca tgagcaagca gggatttgcg gtcgcgtttg tcggcatcgt catcggtttg
     4861 ctgattcttg gcgcgtcccc cttctacatt gtcgatgtca cccagaatgc cattgtggtc
     4921 cagctcggca agccggtccg caatgtcact gaaggcgggc tgtacctgaa ggtgccgttc
     4981 attgaagaag ttacctattt cgataagcgg ctgctggatt atgattccaa cgcccaggac
     5041 gtgatcacgc aggacaaaaa gactctcctg ctcgacaact ttgctaaatg gcgaatcacg
     5101 gatccgctca aggtctacca agcattccag agccagcgcg gggcgctgca acgcctgcat
     5161 gacatcattt attcggaatt acgagtcgaa ctgggccggc acgaattggc agaaatcgtg
     5221 tcatctgccc gggcacaact catggcggtc gtgactcaac gcgccaatga gaaagcgtcg
     5281 g
//
LOCUS       DAOQDS010000133         5047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_10724_length_5047_cov_16.643029, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000133 DAOQDS010000000
VERSION     DAOQDS010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10724_length_5047_cov_16.643029"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PK589_18335"
     CDS             complement(<1..271)
                     /locus_tag="PK589_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21625.1"
                     /translation="MTTLLPLPGRSLLQNLGPCLLLLPILLTACDGHRQRLMTEHYPS
                     YPDSVRWAIDKGNILRGMTEDQVYLALGSPVCKKDVVDEGRTVTVW"
     gene            complement(333..974)
                     /locus_tag="PK589_18340"
     CDS             complement(333..974)
                     /locus_tag="PK589_18340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ21626.1"
                     /translation="MPSTREWRPDDCGDRIRRSSREITQSALLPMESPACYICPMTMQ
                     FQKKDPRATIITPFGEIRIRFYPDDAPRHVENFINLAKMGFYDDTTFHRVVPGFIIQG
                     GDPLSKTPDRLLHGTGGPGYFLNPETNDRPHKRGAISMAKMPRDGNSTRDFNDNGSQF
                     FISLQDNGGLDRRYSIFGEIIRGIEVIDMIAKMPCDERDNPLEPIRMKVTVKE"
     gene            1142..2671
                     /locus_tag="PK589_18345"
     CDS             1142..2671
                     /locus_tag="PK589_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ21627.1"
                     /translation="MPDTAVLNVDLADVWKERGRKNCIRTIAWGDEITVVQQTATYLE
                     VTITFFREKPDGSILPESITGYIEPTKSSGIKVSDVVRPVADNKVLKVNFVDVQQGDG
                     MVIESPDGKVLLIDGGDNQMFARYLAGRFRNTSLQQPKAIDCIIVTHGDADHFAGLSE
                     ILKSETNKVKRKQLFIQPQRIYHNGLVKRPTRMNNKAVPDTKLLGPTKTVGGTLYLTG
                     LEENLLDVDDKDMNEPFREWKQTLAAYNSRSPVSFRRLQLGDTQAFEFFNRNDLRIDV
                     LGPITTDVAGQPALRFLGEPPTGPRIGHDSLNDSDEGFTGHSASHTINGHSIVLRLSY
                     GGFSFLFSGDLNDEASRFLAREHNKGTLNLRSEVFKVPHHGSADFSGAFIQSVSPIVS
                     VVSSGDENARKEYIHPRATLMGALGKWSRVPEPLIFVTELVAFFQTEGPCRLKDDKAA
                     KKRGPFFGFSRTAYGLVKTRTDGHRFFVYTDSGNVQMKEAYAYQLDSSGVPQPAEVNR
                     A"
     gene            2819..3607
                     /locus_tag="PK589_18350"
     CDS             2819..3607
                     /locus_tag="PK589_18350"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HNJ21628.1"
                     /translation="MSDNSKIEWTDATWNPVRGCSKVSPGCTNCYAETFAERFRGVPG
                     HPFEQGFDLRLMPEKLTDPLRWRTPKMIFVNSMSDLFHEQIPDEYIINVARVMRLANW
                     HTYQVLTKRSDRMRDLLNGKLQFASSQSHIWWGVSVENQRHGLPRIEHLREAPARLRF
                     LSVEPLLEDLGTFSLKGIRWVIVGGESGPGARPMAKEWVLSIRDLCKTDHVPFFFKQW
                     GGVRKSETGRILNGKTYNEFPPRSTAPVPSAQELLSIREELEFA"
     gene            3613..4689
                     /gene="tcmP"
                     /locus_tag="PK589_18355"
     CDS             3613..4689
                     /gene="tcmP"
                     /locus_tag="PK589_18355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HNJ21629.1"
                     /translation="MSSDDFHGKPFDEGTVCKLHIFELYAREWFPVFLSSEKPRRKAI
                     HVFDFFAGPGTDRNNVLGSPLRLLSQLQDYQGAQGWEKVAIHAHFYDEDSSKIARLQE
                     NIASRGLRLSNVHYDIRALRFDEAFQGSDSILADTQAAKLVFIDQTGVAHVTTEVFRK
                     LVSSPTCDFLFFLSSSTLHRFRDHPAIWQKIDRPEDYYQVHRAALEYYRGLLPHGQQY
                     YLAPFSIKKGANIYGIIFGSAHPLGIDKFLEVAWRKDEFSGEADFPIDRENIRPGQML
                     LPMVEAQASKITVFENELERLLRDGGLLNEVDVMRVCFEYGVKRRHALPILTKLRKER
                     VIDVDFLVPDIQKFRCPRPIRMRK"
BASE COUNT         1208 a         1396 c         1343 g         1100 t
ORIGIN      
        1 gccagaccgt gactgtgcgc ccctcatcca ccacgtcctt cttgcacacc ggcgagccaa
       61 gcgccaggta cacctgatct tccgtcatgc cgcgaagaat gttgccctta tcaatcgccc
      121 agcgcacgct gtccggatac gaaggatagt gctccgtcat caagcgctgt cgatggccat
      181 cgcaggccgt cagcaagatg ggaaggagca gcaggcaagg ccccaggttc tgtagaagcg
      241 accgacctgg caaggggagg agtgttgtca caataaatca acagagctga cagagtcgtc
      301 gggaaacggc caggcctcat agcacagcga ggttattctt tcaccgtcac cttcatgcgg
      361 atcggttcca gcgggttgtc gcgttcgtca cagggcattt tcgcgatcat atcgatgacc
      421 tcgatgcccc ggatgatttc gccgaaaatc gagtagcgac gatcgagacc gccattgtcc
      481 tgcagcgaaa tgaaaaactg agagccgttg tcgttgaagt cgcgcgtgct gttgccgtcg
      541 cgaggcatct tcgccatcga aatcgcaccg cgtttatgag gccggtcgtt ggtttcagga
      601 ttgagaaagt atccgggacc tccggtgccg tgcagcaggc ggtccggcgt cttgctcaac
      661 ggatcaccgc cttgaatgat gaagcccggc accacacgat ggaacgtggt gtcgtcataa
      721 aagcccatct tcgccaggtt gatgaagttt tcgacatgac gcggggcatc gtccgggtaa
      781 aaacggatcc ggatttcacc gaagggcgtg atgatcgtgg cacgtggatc ttttttctga
      841 aattgcatgg tcatggggca aatgtaacag gccggcgatt ccatcggcaa gagggcggat
      901 tgcgtgattt ctctcgatga acggcgtatt ctgtcgccgc aatcatccgg ccgccattca
      961 cgagtcgatg gcatgagacc gaggcggcca caactatgtg cagactgatc agaatggcgt
     1021 tccggcaagg ccgcaggaag gtcggcgact gaggcgtacc cttgcggtac gtcgcaggga
     1081 gacgaacgac cgagaacgcc gccgggcgtc attctgacca gcctgcaggg ggagagagca
     1141 gatgcctgac acagccgtgc tcaatgtcga tctggctgac gtgtggaagg agcggggccg
     1201 caaaaattgc attcgcacga tcgcctgggg cgatgaaatc accgtggtcc agcagaccgc
     1261 cacctacctc gaagtcacga tcaccttctt tcgcgaaaaa cccgacggca gcattcttcc
     1321 cgaatccatc accggttaca tcgaaccgac caaatcttcc ggcatcaagg tgtccgatgt
     1381 ggtccgaccc gtggcggaca acaaagtctt gaaggtcaac ttcgtcgatg tgcaacaggg
     1441 cgacggtatg gtcatcgaat cgccggacgg caaggtcctc ctcatcgacg gaggagacaa
     1501 tcagatgttc gcccgctacc tcgccggccg tttccgcaac accagtctcc agcagccgaa
     1561 ggccatcgac tgcatcatcg tcacccatgg cgacgcggac cacttcgccg gactcagtga
     1621 gatcctcaaa tccgaaacca acaaggtcaa acgcaaacaa ctcttcatcc agccacagcg
     1681 catctaccac aacggcctcg tcaaacgtcc gaccaggatg aacaacaaag cggtgcccga
     1741 caccaagctc cttggaccga ccaagacggt cggggggacg ctctacctca ccgggctgga
     1801 agagaatctg ctggacgtcg acgacaagga catgaatgag ccgttcagag aatggaaaca
     1861 gactctggcc gcctacaaca gccgcagccc ggtcagcttc cgccgcttgc aacttggaga
     1921 tacccaggcc ttcgaatttt tcaaccgcaa cgacctgcgc atcgacgtgt tgggtcccat
     1981 cacgacggat gtggcagggc agccggcgct gcgttttctc ggtgaaccgc ccacagggcc
     2041 acgcatcggg catgactcgc tcaatgacag cgatgaaggg ttcaccggcc attccgcctc
     2101 ccacaccatc aacggccatt cgatcgtgct gcgcctcagc tacggcggat tcagctttct
     2161 gttttcgggg gacctgaacg acgaggccag tcgcttcctc gcccgcgagc acaacaaagg
     2221 caccctcaat ctcagatcag aagtcttcaa agtgccgcac catggatccg ctgatttttc
     2281 cggtgcgttc attcaatccg tctcgccgat cgtcagcgtg gtgtccagcg gagacgaaaa
     2341 tgcgcgcaaa gaatatattc atccgcgtgc cacgctcatg ggggccctgg gtaaatggtc
     2401 gcgtgtgccg gagccgctca tcttcgtcac cgaactcgtg gcctttttcc aaaccgaagg
     2461 cccatgccgc ctcaaggatg acaaggctgc caagaaacgc ggccccttct tcggcttcag
     2521 ccgcaccgcc tatggcctcg tcaaaacccg caccgacggg catcgcttct tcgtctacac
     2581 cgacagcggc aacgtgcaga tgaaagaagc ctacgcctac caattggaca gttcaggtgt
     2641 gccgcaaccg gctgaagtga accgggcgta aagcgacagg ggaataatcg gtagcgggca
     2701 gttatatgat gtaaaccaag cggtatgctc tccgttgaag tgatgagcga ttgtcagatt
     2761 ctagggatac atattaaact gcttccagtt attcttaaac ttccagggga ccccatctgt
     2821 gagtgataac tcaaagatag agtggacaga tgcgacttgg aaccccgttc gcggttgttc
     2881 aaaagttagc ccgggttgca cgaattgcta cgcagaaacg tttgcagaac gttttcgcgg
     2941 agtgccggga catccatttg aacagggttt tgatttgcgc ctaatgccag agaagctgac
     3001 tgatccgttg cggtggcgca cgcctaaaat gatctttgtt aactcgatga gtgacttgtt
     3061 ccatgaacaa atccctgatg agtacatcat caacgtagcg cgtgtgatgc gattggccaa
     3121 ctggcatacc tatcaggttt tgaccaagcg ttcggatcgg atgagagatc tgcttaacgg
     3181 aaaactacaa tttgcctcgt cgcaatcaca tatttggtgg ggtgtaagcg tcgaaaacca
     3241 aaggcatggt ctcccgcgca tcgaacacct gcgtgaagct ccagcccgcc tccgattttt
     3301 atcggtagag ccgctccttg aagacctcgg gaccttcagt ctcaagggga ttcgctgggt
     3361 tattgtgggt ggagaaagtg ggcctggggc aaggcctatg gcgaaggagt gggtactctc
     3421 gattcgcgat ttgtgcaaaa cagatcacgt cccatttttc ttcaaacagt ggggcggagt
     3481 gaggaaaagt gaaaccggac gaattctcaa cggaaaaacc tacaacgaat ttccccctcg
     3541 aagcaccgcc cctgttcctt ccgcacaaga acttctaagc attcgagaag aactggagtt
     3601 cgcttaggct ccatgtccag cgatgacttt cacggcaagc ccttcgacga aggcacagtc
     3661 tgtaagcttc atatcttcga actctatgct cgcgaatggt ttccggtctt cctttcctca
     3721 gaaaaacctc ggcgtaaagc tatccatgta ttcgattttt ttgccgggcc tggaactgac
     3781 cgcaataacg tcctcggcag cccattgcgg ctattgagcc agcttcagga ctatcaaggg
     3841 gcgcagggat gggaaaaggt tgctatccac gctcatttct atgatgaaga ttcaagcaag
     3901 atagccaggc ttcaagaaaa catcgctagt cgtgggctac gactttctaa tgtacactac
     3961 gatattaggg cactgcgttt tgatgaagca tttcagggct ccgactcgat tctagcggat
     4021 acacaagctg ccaagcttgt tttcatcgat cagaccgggg tcgcgcatgt cacgactgaa
     4081 gtatttcgca aacttgtcag ctcaccaacg tgtgactttc ttttctttct ttcatcctcg
     4141 acactccacc gttttcgtga tcatcccgcc atctggcaga aaatcgatcg accagaagac
     4201 tattatcagg tccatcgtgc agctctggag tattacaggg ggttgcttcc tcatggccaa
     4261 cagtattatc tcgcaccgtt ttcaattaaa aaaggcgcaa atatttacgg catcattttc
     4321 ggatccgccc atccacttgg aatcgataag tttcttgagg tggcttggcg taaggatgaa
     4381 ttcagcgggg aagccgactt cccgatcgat cgggagaaca tacgcccagg acagatgctt
     4441 ttaccaatgg tcgaagctca ggcgagcaag atcacggttt ttgaaaatga gctggagcga
     4501 ttgctgcggg acggaggcct attaaacgaa gtcgatgtaa tgcgggtgtg ttttgagtat
     4561 ggggtgaaga gacgtcatgc tctgccaatt cttacgaaac tgaggaagga gagagtcata
     4621 gacgtagact tcctagtacc cgacatccaa aagtttcgtt gtccacgacc aattcgaatg
     4681 agaaagtagc aaaatgttgc cgctgaacct cccttattcc cgctctcatc cgcctcagag
     4741 ttcaaccaca ttagccccgg cagatatcga agcctgaggc ggctggtgac gacgttgcta
     4801 agtcatagtg aacggcccgg ccatggagca ctgctgtcag aatagtcaaa tcggtgtttg
     4861 tccaaatgtt caggatagtt ccgttttttg ctcacaggca actgcctgta gccaaggagc
     4921 tcacaactgc ctccatgccc ttccttctca ctcgaacacc tgctaagaag tgcaatcatg
     4981 acaaccccta agctgttcga accaatccaa gtcggcgggc tcacgctgag caaccggatc
     5041 gtcattg
//
LOCUS       DAOQDS010000134         4968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_11023_length_4968_cov_20.898433, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000134 DAOQDS010000000
VERSION     DAOQDS010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11023_length_4968_cov_20.898433"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2251)
                     /locus_tag="PK589_18360"
     CDS             complement(<1..2251)
                     /locus_tag="PK589_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21630.1"
                     /translation="MSGTAGTFEQIARGMIDAMAPLRHALSSPDAFRSFMLRLGWETI
                     SVPPAYTALSTTIADAMQAVESLKDDPTAEEVLALLRKAQATYVAIQGIDQAPAGVDA
                     STFLSEMGERLFELLLTDYLAAHQPALFNLLSILNVIETEQIAATANQRAHVRTHFHW
                     ANIPEIIRDPASLPQRVYGWGTQELKFQLILQHFSELLFAFGFPVFIGKAEELLANGY
                     AGEDIDLDEETRWLKVPFYYITIADRDLEAAISVVELKGNGTTLPGLAIQPHIPSEFP
                     LTLHLAPTIDFRLRAGTNAATQVGIVLRPGDASIKYPFEPGTTPPSAGIGLGFDFKPS
                     SPTLLFGSPNATRLELQGASVDLGASFVQNELDVQVAAQLKGLTLVLQPGEADSFLRN
                     LFGDSERRLTIPLGFEWSRRNGVHFAGSDAFETSLPANLSLGPVTVQGLTVQLFAPTD
                     HSADLTLGVGAQISGTLGPVSFAVEGMGFRLQTTFAPGNAGPFDLSLGFKPPTGLGLA
                     ISTPGVTGGGFLGFDPQRAEYSGMLQLELAETLALKALGLLTTRLPDGSRGYSLIVLL
                     TAEGFAPIPVGLGFTLTGIGGLVALHRTVRTDVLREGLKTGTLNAILFPPDPLRNAPQ
                     IFSDLRRVFPPTAGRHVVGPMVQLRWGTPTLLTLDLALLVELPAPIRVVVLGRLQVLL
                     PDQSHPLVQIRMDALGVLDLSAETVALDATLYDSRILQFTLTGDMALRAGWGRQPQFV
                     LAIGGFHPRF"
     gene            complement(2248..4821)
                     /locus_tag="PK589_18365"
     CDS             complement(2248..4821)
                     /locus_tag="PK589_18365"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HNJ21631.1"
                     /translation="MPITGIQWKSNREYDIHLLRGRTLSPLLAQVNVELPDGNTQDAA
                     TYLAANADVTIDFQPSFRNVLDVTATPPTCTGFGITINNTNGEVRVPSPPTPAPVIHN
                     FLLHATAQDSSDGKEYRISVRIHLHNRVTSAWLTPPILTLRPDGPTLPQTTFRRFTVR
                     AQFDDNTVGDLTNQQGLTWGPLGNVEPSGRLVISVGNSPSDPAVEITATLPADLRDPG
                     NPTPPDIVARGHIRFAADWASESTIKTETVQVQDTWPGTINPELVPNFLFLSDGYLAE
                     DKPQFESQIRSLLGLMKKSRLTRPFDLLSTSMNYFQAFVPSSHHGISVLCEVYPAQKK
                     SGEVQTNSDDTVNLYCIPDPSDPAAGARWDLSNLLFRLGLPVPGQGLDRPVKEIRDYW
                     DSILDAVPHDRISNETVRRWQKLARRTFLEETDSTFGLAYGDYPNVTNESDSREVGFH
                     PRRMSRERLDTILNRLHDSHGNPMGQLWAARADGTRPNSYPLIFLFSSLKWDRGVNYG
                     RSYIAMNVEERYEIPARPVSGKPTYGIDLTGRITKNISHGRLIRGCHEVAHSFGLGDE
                     YSEKGTLPQSREIDRFYGNLQKHNDLLDSFKDIDGDLIKWRWHRIRKATVLMGAISEG
                     PSGVFRIPIPLGQSRQFKQGNTVLLRARRYPIPLPRNPDTSEHLQIVGLADPGGVADL
                     SKPEGPDNPLGAAIMVSPKSGHSFTAADAARFGAGCVLYLPVEASESARSEDYPFAEL
                     IALNVKDHITDRGCALNQDPDSDEICVPDKNDIQKPKKLDIDFPRCFKHKNRIVGLFT
                     GGKTFHCGIYHPTGNCIMRNSDSDGKEFCPVCRYLLVDIIDPHQHFSIDLDYGEIYPQ
                     K"
BASE COUNT          968 a         1348 c         1654 g          998 t
ORIGIN      
        1 caaaccgtgg gtggaagccg ccgatggcca gcacgaattg cggctgacgg ccccacccgg
       61 ctctcaacgc catatcaccg gtcagggtga attgtagaat gcgggagtca tacaacgtgg
      121 catccaaggc cacggtctcg gcgctgagat ccaggacgcc caaggcatcc atgcgaatct
      181 gcacgagcgg atgagactga tccggcaaca ggacttgcag ccggccgagc acgaccacgc
      241 gaatcggcgc gggcagttcg acgagcaggg ccaggtcgag cgtgagcaat gtcggcgtgc
      301 cccagcgcaa ctgcaccatc gggccgacca catggcggcc ggcggtgggc ggaaacacgc
      361 ggcgcaaatc gctgaagatc tgcggggcgt tccggagcgg gtcaggtgga aacaagattg
      421 cgttgagcgt cccggtcttg aggccttcgc gcagcacgtc ggtgcgaacg gtgcggtgca
      481 gcgcgaccaa gccgccgatg ccggtgaggg tgaagcccaa gccgacagga atgggcgcga
      541 agccttcggc ggtcaagagc acaatcaacg agtagccgcg gctgccgtcg ggcagtctgg
      601 tggtgagcag gcccagggct ttgagcgcga gggtctcggc cagctccagc tgcagcatgc
      661 cgctgtattc ggcccgctgg ggatcgaagc ccaggaaccc gccgccggtt acccccggcg
      721 tactgatcgc caggccgagg cccgtcggcg gtttgaagcc gagggagagg tcgaacggac
      781 cagcgttgcc cggagcaaag gtcgtttgaa gacggaaccc catgccttct accgcaaacg
      841 agacggggcc gagtgtgccc gagatctgcg cccccactcc aagggtcagg tccgcggaat
      901 ggtcggtcgg cgcaaagagt tggaccgtca gcccttggac cgtgacgggt ccaagcgaaa
      961 gattcgcggg caaggatgtc tcgaaggcgt cgcttcctgc gaagtgcacg ccgttgcgtc
     1021 gagaccattc gaaaccgagc ggaatcgtga gccggcgttc gctatcgccg aacaggttgc
     1081 gcaggaagga atcggcctca cccggttgca agacaagcgt caggcctttg agttgcgcgg
     1141 ctacctggac gtccaattca ttctggacga aggaggctcc cagatcgacc gatgctcctt
     1201 ggagttcaag acgggtggcg ttcggtgaac cgaacagcag ggtcggggac gatggtttga
     1261 aatcgaaccc gagcccgatg ccggccgaag gcggtgtggt acccggttca aatggatact
     1321 tgattgacgc gtcgccgggg cggagcacga tgccaacttg agttgcggcg ttggtgccgg
     1381 cacgcaggcg gaaatcgatg gtcggcgcca ggtgcaaggt cagcgggaat tcgctcggaa
     1441 tgtggggttg gatggccagg ccgggcaggg tggtgccgtt gcctttcagt tctaccacac
     1501 tgatggcagc ttcgaggtcc cggtcggcga tcgtaatgta atagaaggga accttgagcc
     1561 atcgcgtctc ttcatccagg tcgatgtcct cacctgcata tccgttggcc agcaactcct
     1621 ccgccttgcc gatgaagacg gggaaaccga atgcgaacaa cagttcgctg aagtgctgga
     1681 gaatgagctg gaacttgagc tcctgcgtgc cccatccgta cacccgttgc ggaagggatg
     1741 cgggatcgcg aatgatctcc gggatattgg cccaatggaa gtgtgtgcgc acgtgcgcgc
     1801 gctgattggc cgtggccgcg atctgctccg tctcgatcac attgaggata gagagcagat
     1861 tgaacagcgc cggttgatgc gcggcgagat agtccgtcag gaggagttcg aacagtcgtt
     1921 cgcccatctc gctcagaaag gtgctcgcgt ccacgccggc cggagcctga tcgataccct
     1981 ggatggcgac gtaggtcgct tgggcttttc tgagcagggc cagcacctcc tccgctgtcg
     2041 gatcatcttt gagggattcc actgcctgca tggcgtcggc gatcgtcgta ctgagcgcgg
     2101 tataggccgg gggaacggag atggtttccc agccgaggcg gagcatgaag gaccggaagg
     2161 catcgggact gctcagggca tgccgcaacg gcgccatcgc gtcgatcatg ccgcgtgcaa
     2221 tctgttcgaa tgtgccggcc gtgccgctca tttctgcggg taaatctctc cgtagtccag
     2281 gtcgatggaa aaatgctggt ggggatcgat gatatccacc agcaggtaac ggcacacggg
     2341 gcagaactct tttccgtcgc tgtccgagtt acgcatgatg cagttgccag tgggatgata
     2401 aatcccgcaa tggaacgtct ttcctcccgt gaacaagccg acgatccggt ttttgtgttt
     2461 gaagcagcgg ggaaaatcga tatcgagctt tttcggcttc tgaatgtcgt tcttgtccgg
     2521 cacgcagatc tcatcactgt caggatcctg gttgagcgcg cagccgcggt ccgtgatgtg
     2581 atccttgacg ttcagggcga tcaactcggc aaaagggtag tcttccgatc gggccgattc
     2641 actggcctcc accggcaggt agaggacgca accggccccg aaccgggccg catcggcggc
     2701 ggtaaatgag tggccggatt tgggagagac catgatggcc gcgcccaagg gattgtccgg
     2761 accttccggt tttgagagat cagccactcc gcccgggtcc gctagtccga cgatctgcag
     2821 gtgttcgctg gtgtccggat tgcgcgggag gggaatggga tatcgtcgag cacgcaggag
     2881 tactgtgttg ccctgtttga actggcggct ttggcctaat gggatcggaa tacggaagac
     2941 gccggagggt ccttcgctga tggcccccat gagcacggtg gctttgcgga tgcgatgcca
     3001 gcgccatttg atgagatcgc cgtcgatgtc tttgaaagaa tcgagcaggt cgttgtgttt
     3061 ctggaggttg ccgtagaacc ggtcgatttc acggctttgg ggcaacgtgc ccttttcgct
     3121 gtattcgtcg cccaggccga aggaatgcgc cacctcgtgg caaccgcgga tgaggcggcc
     3181 atgcgagatg ttcttggtga tgcggcccgt gagatcgatg ccatacgtcg gtttcccgga
     3241 gacgggcctc gcagggattt cgtagcgctc ttcgacattc atggcgatat aggagcgccc
     3301 gtaatttacg ccgcgatccc atttgagaga ggagaagagg aagatcagcg ggtagctgtt
     3361 gggacgcgtg ccatccgctc gtgcggccca gagttggccc atgggattgc catggctgtc
     3421 gtgcaggcgg ttcaagatcg tatccagccg ctcccgagac attcgtcgcg gatgaaagcc
     3481 gacttcgcga ctgtcgcttt cattggtgac gttggggtaa tcgccgtagg ccaatccgaa
     3541 cgttgaatca gtctcttcga ggaacgtccg tcgtgccaat ttttgccagc gtcggacggt
     3601 ctcattggaa atcctgtcat ggggcacagc gtcgaggatg ctatcccagt agtcgcggat
     3661 ctctttcacg gggcgatcga gcccctgccc gggaacgggg aggccgagcc tgaacagcag
     3721 gttgctgaga tcccagcgtg cccctgcggc ggggtctgag ggatcaggga tgcaatagag
     3781 attcacggtg tcgtcgctat tggtttgcac ctccccgctt ttcttctgcg ccgggtacac
     3841 ctcacaaagc accgaaatcc catggtggct ggacggcacg aaggcctgaa aataattcat
     3901 cgacgtggag agcaagtcga acgggcgcgt cagcctgctc tttttcatca acccgagcag
     3961 gctacggatt tgggattcga actgtggttt gtcctcggcg agatagccat cggacaagaa
     4021 cagaaagttc gggacgagtt cggggttgat cgtgccaggc caggtgtcct gcacctggac
     4081 ggtctccgtt ttgattgtgc tttccgacgc ccaatcggct gcgaaccgga tatggccgcg
     4141 cgctacgatg tccggtggtg tgggattgcc gggatcccgt agatcggctg gaagcgtggc
     4201 ggtgatttcg acggcggggt cgctggggct gtttccgacc gaaatgacca atcggccgga
     4261 tggctccacg ttgcccaatg ggccccatgt cagtccctgc tgattggtca ggtcgccgac
     4321 ggtgttgtca tcgaactgtg cgcgcaccgt gaatcgtctg aacgtggttt gcggcaaggt
     4381 cggaccgtcc ggccgcaacg tcaggatcgg cggcgtcagc caggcggaag tcacacggtt
     4441 atgaaggtga atccggacgc tgatgcggta ttccttcccg tcgctcgaat cctgcgccgt
     4501 ggcgtgcagc agaaaattat gaatgaccgg cgctggagta ggaggcgacg ggacacggac
     4561 ttcaccgttg gtattgttga tggtgatgcc gaagccggtg caggtcggcg gtgtggcggt
     4621 cacatcgagg acgttgcgaa acgaaggctg gaaatcgatg gtgacatcgg cattggccgc
     4681 taaataggtt gctgcatctt gcgtgtttcc atccgggagt tcgacattca cttgagccag
     4741 aaggggagag agggtcctgc cgcgcagcag gtggatgtcg tactctcgat tgcttttcca
     4801 ttggattccg gtaatcggca tggtgactag tgctgtctgg tgatctctga acggtgacgg
     4861 aataagaaaa tacgttgcct cgcacgacgt cgtcgaacag gatcagacac gatcatgaag
     4921 ggatgagccg aaaggtactc cgctcgtgat ggcctgtcaa actcgtct
//
LOCUS       DAOQDS010000135         4786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_11727_length_4786_cov_20.243078, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000135 DAOQDS010000000
VERSION     DAOQDS010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11727_length_4786_cov_20.243078"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..648
                     /gene="moaC"
                     /locus_tag="PK589_18370"
     CDS             148..648
                     /gene="moaC"
                     /locus_tag="PK589_18370"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNJ21632.1"
                     /translation="MADFTHFNESGRARMVDVGAKASTERLATAQAIVFLQPETLEKI
                     QQGKIAKGDVLSVAQVAGVMGAKKTPDLIPMCHPILLTSVDISFQEIAQRDREGRCSI
                     VISATAKTTGPTGVEMEAMTAASVAALTIYDMCKAVDRGMSFGDICLLTKSGGKSGTY
                     QRPVPV"
     gene            658..921
                     /locus_tag="PK589_18375"
     CDS             658..921
                     /locus_tag="PK589_18375"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNJ21633.1"
                     /translation="MVTVRLFGMTKMLAGNQGALSLALPEGRRVKDLVSVIEAAYPKI
                     GELLHKKKVLVSVNQDIAHEDLEVRDGDEIALLPPFAGGSAGC"
     gene            1070..1543
                     /locus_tag="PK589_18380"
     CDS             1070..1543
                     /locus_tag="PK589_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNJ21634.1"
                     /translation="MSLEQVTKADQPVTGDEAMLVRVQREDFSIDEELCRVRARSKRI
                     GGIAIFLGTARDRSKGKDVDGITFEHYEGMAQKKLREIRERALKDFGIIELSILHRYG
                     EIGIGDNIVLIIAGAEHRAEAFQACKWAIDELKQITPIWKLEHTPEGEVWVEEHP"
     gene            1591..2652
                     /gene="moaA"
                     /locus_tag="PK589_18385"
     CDS             1591..2652
                     /gene="moaA"
                     /locus_tag="PK589_18385"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNJ21635.1"
                     /translation="MDHTPDTPVPTIYDRLGRPLRSLRLSVTDRCNLRCKYCMPEDDY
                     AWLPRDTILTFEEMAELTAVFTELGVDKVRLTGGEPLLRRDLPRFVRQLSENRRITEI
                     ALTSNGVLMADQAADLSFAGLNRVTISLDTLRADRFRTLTKRDLHHQVFDGIKAVVQA
                     GFPSLKFDTVVIKGYNDDELIDLIEYGKTVGGEVRFIEYMDVGGATDWSMKQVLSKAE
                     MLELVGRHYGGVKPVVENSAAPAERFVLPDGTIFGIIPSTTTPFCRACDRSRLTADGM
                     WYLCLYAKDGLDLRAPLRQGRTRDELKALIIAAWEGRADRGAEDRKALEVLGLREQRL
                     IEINRLREDPHLEMHARGG"
     gene            2713..3420
                     /locus_tag="PK589_18390"
     CDS             2713..3420
                     /locus_tag="PK589_18390"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21636.1"
                     /translation="MFDPHTLTILSLGFVLGLRHALDADHLAALSTVLAERPTVQAST
                     AIGFFWGLGHTLMLLCVGTLLLALNLTIPESMANLFEFAVGGMLVALGLSLARRVYRE
                     QWHVHTHEHGGRPHVHLHSHHLQADHTHGHWYQGSLRPLAIGMAHGLAGSAALMLVVL
                     STVTGMAQGIGYIVVFGVGSILGMVGVGAVLSLPVIYSVTVGPRAYWMVQGLTCLASI
                     GLGVVMMVRIGLAGGWS"
     gene            3620..4408
                     /locus_tag="PK589_18395"
     CDS             3620..4408
                     /locus_tag="PK589_18395"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HNJ21637.1"
                     /translation="MNVRVLGCHGSGQLVPGENGPVQCGTCGFLINEELLVDAGTVGS
                     RLYLEEQRRIRLVLLTHLHFDHIRELPTLADNLVGEIEEPVVIAAIPEVLDGLRRHIF
                     NGTVYPDFFRLPDPVKPVFVSRELLPGQETLLCGLGVTPIPVNHVVPTVGFLIREQRR
                     TVLFSGDTYQTDALWQAAGDVDALQAAFIECSFSDEMAELARVAKHLTPALLAEEFAK
                     LAKPEVPVYAYHLKPRFRAQIRQELGGLGIRHLTALEEGQTLVL"
     gene            4419..>4786
                     /locus_tag="PK589_18400"
     CDS             4419..>4786
                     /locus_tag="PK589_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HNJ21638.1"
                     /translation="MAWFKKGKTEEAEPPKRSKTAEGMWLKCNHCREIVYRKEVDRNN
                     KVCPKCDYHFPISVIERINLLVDLGTFKEWDPELEPQDPLQFQDTRSYKDRIKAQQEK
                     TGRKDAMVIGQGVINGRKVAL"
BASE COUNT          946 a         1280 c         1506 g         1054 t
ORIGIN      
        1 ctcggaccag atactcgcct gccgttcaac gcgctcgatt tcgttgacga ggcgcgggca
       61 tcggccggat gtcgcttgac tcccattttc cccggctcct ataatttggc ggttttgatg
      121 cgacaaccag ctgatttggc ataaagaatg gccgacttta cgcatttcaa cgaatcgggg
      181 cgggcgcgga tggttgatgt cggggcaaag gcctcgaccg agcgcctggc caccgcgcag
      241 gccatcgtct tcctgcaacc ggaaaccctt gaaaaaattc agcaaggaaa gatcgcaaag
      301 ggcgatgtgt tgtctgtcgc ccaggtggcc ggcgtcatgg gggcaaagaa gaccccggat
      361 ctcattccga tgtgtcaccc cattttgttg acgagcgtcg atatttcgtt ccaggaaatc
      421 gctcaacggg accgtgaggg gcgctgctcg atcgtgatca gtgctaccgc caagactacg
      481 ggcccgacgg gcgttgaaat ggaagccatg acggccgctt cggtggccgc gttgacgata
      541 tatgatatgt gcaaggcggt cgatcggggc atgagcttcg gcgacatctg tctgctgacg
      601 aagtcgggcg gcaagtccgg cacctatcaa cgtccggttc cagtgtaagg gcgtgacatg
      661 gtaacggttc gactcttcgg catgacgaaa atgttggcgg gaaaccaagg tgccttgtct
      721 ctggccctgc cggaaggccg acgggtgaaa gacctggtgt ctgtgatcga agccgcctac
      781 ccgaagatcg gtgagttgct ccacaagaaa aaagtgctcg tttcggtcaa tcaggatatc
      841 gcgcatgagg atcttgaagt gcgtgacggg gatgaaattg ctttgctgcc gccgtttgcc
      901 ggcggaagtg ccggatgctg aacaagcctg ccagcggcgt tctcgctttg cgcgtcggct
      961 cgacgtactc aaaggtacgc ctcgcctccg tgcgcgctgc ggccttgccg gacaggcttg
     1021 ttgagcatcc tttccgcaga gccaacggga aaccggaggg agagaatcga tgagtcttga
     1081 acaggtcacg aaagccgacc agcccgttac cggcgacgag gcgatgctgg ttcgggtgca
     1141 gcgagaagat ttctcgatcg acgaagaact ctgccgtgtc cgcgctcgtt ccaagcgcat
     1201 cggcggcatt gcgatctttc tggggactgc cagagaccgg tcgaagggaa aagatgtcga
     1261 cggcattacc ttcgagcatt acgagggaat ggcgcagaaa aaattgcggg agattcgtga
     1321 acgcgcgctg aaggatttcg ggatcatcga actgtcgatc ttgcaccgat atggcgagat
     1381 cggcatcggc gataacattg tattgattat tgccggtgcg gagcatcgag ccgaggcctt
     1441 tcaggcctgt aagtgggcca ttgatgaatt gaagcaaatc accccgatct ggaaactcga
     1501 gcacactccg gaaggcgagg tgtgggtcga ggagcaccct tagcctatcc cgacagcaat
     1561 agggaaagag gcccgcgtcc tcgacatatt atggaccaca cgcccgatac acccgttcct
     1621 acgatatacg accggctggg acgtccgctc cgcagcctgc gcctgtccgt gacggaccgc
     1681 tgcaatcttc ggtgtaagta ttgtatgccg gaggatgatt acgcctggtt gccgcgcgat
     1741 accattctga ccttcgagga gatggcggag ctcactgcag tctttaccga gctcggggta
     1801 gacaaggttc gtctgacggg tggggagccg ctgttgcggc gggacctgcc ccgatttgtc
     1861 cggcagctgt cggaaaatcg acggatcacg gagattgccc tcaccagcaa cggcgttctc
     1921 atggctgatc aggccgccga tctctcgttc gccggtctga atcgcgtgac catcagcctc
     1981 gataccctcc gtgcggaccg attccgtact ttgaccaagc gggatctcca tcaccaggtg
     2041 tttgacggca tcaaggcggt ggtgcaagcg gggtttccgt cgctgaagtt cgacaccgtc
     2101 gtcatcaagg ggtacaacga cgacgaactc atcgacctga tcgaatatgg caaaacggtc
     2161 ggcggcgaag tccgattcat tgaatacatg gatgtcggcg gcgccaccga ttggtccatg
     2221 aagcaagtat tgtcgaaggc cgaaatgctc gaactggttg gccgccacta tggcggcgtc
     2281 aagccggtgg ttgagaacag cgctgcgccg gcggagcgtt ttgtgcttcc ggatggcacc
     2341 atcttcggca tcattccctc gacgaccaca cccttttgcc gtgcctgtga tcgcagccgg
     2401 ttgacagccg acgggatgtg gtatctctgt ctctatgcga aagacgggct cgatctccgt
     2461 gcaccgctcc ggcagggccg aacccgggac gaattgaaag cgttgatcat cgcggcctgg
     2521 gaaggtcggg cggatcgtgg cgccgaagat cgcaaagcgc tggaggtgtt agggttgcgt
     2581 gagcagcgcc tcattgaaat taatcggctc cgagaagatc cccacctgga aatgcatgcg
     2641 cgtgggggat gaccgccagt gtgtgctgct cccactgccg gatccgctga tgaccctctc
     2701 ctggagcagt cgatgtttga tcctcatacg ctgactatcc tgagtcttgg attcgtgctg
     2761 gggcttcgtc acgccctgga cgcggatcac cttgccgcgc tttccactgt gctggcagaa
     2821 cgtcctaccg tacaggcgtc cacggcgatc gggtttttct ggggacttgg ccacacgctg
     2881 atgctgttgt gcgtcgggac cctcttgctg gcgttgaacc tcaccattcc ggaatccatg
     2941 gcaaatctgt tcgaatttgc cgtcgggggc atgctcgtgg cgctggggct ttccttggcc
     3001 cgccgggtct atcgtgagca gtggcatgta cacacccatg agcatggggg ccggccgcac
     3061 gtccatctcc acagtcacca cttgcaggcg gatcacacac acgggcattg gtatcagggc
     3121 tcgttgcggc ctctggcgat cgggatggca catggattgg ccggttcggc agcgttgatg
     3181 ttggtcgtat tgtcgacggt gacggggatg gcccagggga tcggctatat tgtggtgttc
     3241 ggtgtcgggt ccattcttgg gatggtgggt gtcggggcgg tactcagtct gccggtgatc
     3301 tattctgtga cggtcggtcc tcgtgcctac tggatggtgc agggactcac ctgtcttgcc
     3361 agcatcggcc ttggggtggt gatgatggtc cgaatcgggt tagccggtgg ctggtcctag
     3421 ccgcttggtt cgtcgcagat cttccgcgct cctgcccaca ctcccttttt ccatgacacg
     3481 cctcggtaac gaatgaccta tgtaaatctg tgtgcgcctt tttcagaggt atgttattct
     3541 gccgcccagt gcggaggact cgtctacaat atcgtaaaaa catgctgaca cgccggggca
     3601 cgtccggaga gcctcggcca tgaatgtacg tgtattgggt tgccatggtt cggggcagct
     3661 tgttccgggt gagaatggtc cggttcagtg cgggacctgc ggctttctga tcaatgagga
     3721 attgctagtt gatgcgggaa ccgtcggatc ccggctctat ctggaagagc agcggcggat
     3781 ccggctggtc ctcttgaccc acctgcattt tgatcatatt cgcgagttgc cgacactggc
     3841 cgataacctg gtcggtgaga tcgaggagcc ggtcgtcatc gcggcgattc ccgaagtttt
     3901 ggacgggttg cgtcggcaca tttttaacgg cacggtctat ccggattttt tccggttgcc
     3961 cgatcctgtg aagccggtgt ttgtgtcccg tgaacttctc ccggggcagg agacgttgtt
     4021 gtgcggtctg ggagtgacgc cgattccggt gaaccatgtg gtcccgacgg tgggatttct
     4081 gatccgcgaa cagagacgga cggtgctgtt tagcggtgac acctatcaga cggacgcgct
     4141 ctggcaagcg gccggggatg tggatgcatt gcaggcggca tttatcgaat gttcgttttc
     4201 cgacgagatg gccgaattgg cacgagtggc gaaacacctc acgccggcat tgctggccga
     4261 ggagtttgcc aagcttgcga aaccggaggt gccggtctat gcctaccatt tgaagccgcg
     4321 gtttcgagca cagatccggc aggaactcgg aggactcggc attcgacatt tgaccgcgct
     4381 tgaagagggc caaacccttg tactataggt gagcagagat ggcttggttt aaaaaaggaa
     4441 aaactgagga agcagagcca cccaaacgct cgaaaacggc ggaggggatg tggctgaagt
     4501 gtaatcattg tcgggagatc gtgtatcgca aggaggtcga tcgtaacaat aaggtctgcc
     4561 ccaagtgcga ttatcacttt cccatctcgg tcatcgaacg catcaatctg ttggttgatc
     4621 tcggcacgtt caaagagtgg gatcccgagc tcgagccgca ggacccgctg cagtttcagg
     4681 acacccgcag ctataaagac cggattaagg cacagcagga gaagacgggt cggaaggacg
     4741 ccatggtcat cggccagggt gtcatcaacg gacgaaaggt cgccct
//
LOCUS       DAOQDS010000136         4719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_12016_length_4719_cov_19.117710, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000136 DAOQDS010000000
VERSION     DAOQDS010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12016_length_4719_cov_19.117710"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PK589_18405"
     CDS             <1..306
                     /locus_tag="PK589_18405"
                     /inference="COORDINATES: protein motif:HMM:NF023087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraM"
                     /protein_id="HNJ21639.1"
                     /translation="QVMLDQYKEELEALALRWGNDARDKSERILNASLMASKGAMAKV
                     MQEGAREAAGVVRGEVDAALGRVAGAMRDARRIGALNVVASCITCLAAAVALWAAVH"
     gene            1504..2889
                     /locus_tag="PK589_18410"
     CDS             1504..2889
                     /locus_tag="PK589_18410"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNJ21640.1"
                     /translation="MRIHQEIWDGDDPTDHGQIYEFLRERLPTVRRRFADQELVIHVS
                     PGTPSMQTVLILMGETGFIDSPFSLVKSYRKDERNGRPAIVPVWLGIESYYKAYRSAR
                     PAEMSSEDAAVVWDPARFQSERMRQIFDEARRFAQLNVPVLLLGERGTGKTTLAGWIR
                     SHSPYRVEERDAHWPAVACGQYNPETMRAELFGYKRGSFTGALKDHEGLLAVAHKDTL
                     FLDEVGDIGRDLQRLLIKAVEEKSYMRLGDDRQLTSDFRLISATNLTHDKLRERLDPD
                     FLDRISMLTLRLPPLREIPEEIPWLWETVFSQASHRAGSGRVRLPAGAHRAIVDELSR
                     HPLPGNLRDLFRVAYRTIAATADRHSPLSPTASSQYGLAGIGGSGAGIGTDSLPRSVA
                     RAFAESASLDALIQPGETIKTKVIERVLKRYLADEFRRIAKKRGVSPETLCDVTDRAL
                     REWSASKDWNT"
     gene            3467..4552
                     /locus_tag="PK589_18415"
     CDS             3467..4552
                     /locus_tag="PK589_18415"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ21641.1"
                     /translation="MGWAKYHEDNVSRFWRDANTQVRVVHGTQDRPASKAGAGDDLGE
                     LKLLRRRLAENPCDRSAQLTFHAEKSRLEKLGISTGEILGVIHGDDRRADGPMDIASP
                     NGGTSLVQRQLQNEIGRNAQPNSPKALAKAQLNKQGERNMSKLKDFTVSTARPLPVIV
                     LADVSGSMSANGKIDALNDAIQSMIESFAAEDHSRAEIHVAVIAFGKGSARLHQALLP
                     AAQIKWERVGAAGNTPMGAAFDLVVKMVEDQAQIPSRAYRPTVVLVSDGQPTDEWREP
                     LQRLLASDRASKASRFVLGIGDDADPAMLAEFLADPSARAYSAHEARQIKNFFRWVTM
                     SVTQRTRSVNPNSVVVVHPTALDDYDF"
BASE COUNT         1001 a         1317 c         1481 g          920 t
ORIGIN      
        1 caagtcatgc tggatcagta caaggaagaa ctggaagcct tggcgctgcg ctggggtaat
       61 gatgccaggg acaagtcgga gaggatcctg aatgcgtcgc tgatggctag caagggagcg
      121 atggcgaagg tcatgcagga aggggccagg gaagcggcgg gggtggtgcg tggggaagtc
      181 gatgcggcgc tggggcgggt tgccggcgcg atgcgtgatg ctcgacggat cggggccttg
      241 aatgtggtgg cgtcgtgcat tacgtgcctg gcggcagcgg ttgcactgtg ggcggcggtg
      301 cattgaagag cgccctgccc gccgcaaacc atccagaaat tgagagttga aagaccaaac
      361 caccggctac gcctcgagtc atcgccgcgc ccggaacgtg aaagcgaacg cctgcgggat
      421 cttctgactg ctgacaagcg tgaggagatc tttgctatcg agatcatcgt ctatgctaag
      481 ccattaactt cacttggaac gacgcgaagc atcatctacc gtctcgcaaa cttcaagctt
      541 gaagcggtca gctcacgcaa cggaaaaaat cgtacccaaa ggtgcgtgac ggccttcgcc
      601 tagcagtagt gcaaaaccgc gtctgcaaga catgacatgc aggaggggca gtttggaccc
      661 agaagagcca ttcggcttct caagcgaaaa atttcccagc gcgaagagtg gctaacagat
      721 tcgaaaaccg ccgctccgct ctgtgcgctg tcggcccaga tctcggctac tgctgctgtt
      781 cgcgcctcgg ctgtgtgcgg tcagcaattt gcttgttagc cgacggcggc gtcggattgg
      841 tggatccaat tctcaccgat gatcatgaat gcgtccttct cctcttgaga ttggtacggc
      901 cggcaggggt agtcactatg gattggtcca tcggaccaat ccgagcatga cgaacaggtg
      961 gttgctgtac aatcggcatt gcccaacctg aaatctggcg tcacgccggt ttatgaatgg
     1021 gctaccaaca ctctcgcccc agttgactgc gaggcgtgca cgaatcgcgt gaaagtcgga
     1081 tctgagttgc cgcggtaggc tcgagagcgt gccttgttga acattggtgc gcgggcagca
     1141 gcgagtaccg cgggtgggaa gacgggggga ggatctcatt tcatgcatct ggaagccaag
     1201 gcaagggcta tgcatccacc ggtgggctag ccacataaca tgcgtcgtcg agttctgatc
     1261 tcttggatag ccgtaaacaa tgatccctac gagcgtgatc gggcaggtag tgcctttcgt
     1321 cttgtcgatg gatcaccggt tcttggcccc actttgacct tgttgttcga tccggactca
     1381 gcttacgccg gaagcatttc tgacttcgtt ctgttccgcg gaccttcgcg agaaaggggg
     1441 gagacacgcg aatctcgcgc ggtcagtcag acgttggagg aactgagggc cagggatcct
     1501 ggattgcgca ttcatcaaga gatctgggac ggtgatgatc ccaccgatca cggacagata
     1561 tacgagtttc tgagggagag gctccccacg gtacggagaa ggttcgccga ccaagaattg
     1621 gtaatccacg tcagtcccgg cacgccttcc atgcaaacgg tcttgatact gatgggcgaa
     1681 actgggttta tcgattcccc gttttccctc gtcaagtcct atcgtaagga cgagcggaac
     1741 ggtcgtcctg ctatcgttcc cgtttggctc ggcatagaaa gctactacaa ggcataccgt
     1801 tcggctcggc ccgccgagat gtcctcagaa gatgctgcgg tcgtctggga tcctgcgcgg
     1861 tttcagtcgg agcggatgcg ccagatcttc gacgaggccc gtagatttgc gcagttgaat
     1921 gtacccgtcc tcttgctcgg tgagcgaggc acgggcaaga caactctggc tggctggatt
     1981 cgatctcaca gtccctaccg cgtagaggaa cgagatgctc attggccggc ggttgcgtgc
     2041 ggccagtaca acccagaaac gatgcgtgcc gagttgttcg gctataagcg tggttctttc
     2101 actggcgccc tcaaggacca cgaaggcctg ctcgcagtgg cccacaagga cacactgttt
     2161 ctcgacgaag taggcgacat cggccgagac ctccagcgcc tcctgatcaa ggccgtcgag
     2221 gaaaagagct acatgcgcct gggcgacgat cgacaactga cgagcgactt cagactgatt
     2281 tcggcgacta acctcacgca cgacaagttg cgcgagcgat tggatccgga ctttcttgac
     2341 cgcatcagca tgctcacgtt gcggctgcca cccctgcgtg agattcctga agagattccg
     2401 tggctctggg aaacggtttt ttcacaagcc tcacataggg caggtagtgg ccgcgtccga
     2461 cttccggccg gtgcccaccg ggccatagtg gacgaactat cgcgtcatcc gcttcctgga
     2521 aacctgagag acctatttcg cgtggcgtac cgcacgatcg cggccacggc cgaccggcat
     2581 tcaccgctat caccaactgc ttcgtcccag tacgggcttg cgggcatcgg aggatctggc
     2641 gctggcattg ggaccgactc gttgccgagg tctgtcgccc gcgcattcgc ggagtcggca
     2701 agcctggatg cgctgatcca gcctggtgaa accatcaaga cgaaggttat cgagcgagtt
     2761 ctcaagagat acttggcgga cgagtttcgc cggatcgcga agaagcgggg cgtgtcacct
     2821 gagacccttt gcgacgttac tgatcgcgcg ttgagagagt ggtcggcaag caaagactgg
     2881 aacacttgat ccgcatgccg gaaataatct gccgcaaagg ccgagtattt gggctacctt
     2941 cggcccgctc aacatgtgcg ctcattggat acaagcgcct gttaaataat gcattattga
     3001 catattggtg tggggtatcg accccattgg cacgcttgtt gcgatatcgg ctggtgtggg
     3061 actcggaatc gttccgggtc cgatcaaccc aggagtgcca gccaaatgaa caagcacaac
     3121 aacgcagcga gcaacgcccc ggccatcagt cagcacgcct ggactcgcat gggtgggcgt
     3181 ggagtgaact gccagactat cgataccgtc ctcagctatg ggcgggtaat ttacatacgc
     3241 ggggcagcaa tccacgtcat cggcaggaag gaagtggaga agctgtcgtg ttccggcgtg
     3301 gatgtggccg gttgcgaggg tgtccaagtt gtctgcactc ccgacggttc cgcaatcctg
     3361 acggtgtaca agaacagcga cttccgagga ctgaagccgc gtggcggaag gcgttggcac
     3421 taatggccga ccgctcattc gctcggaact aggcaggggg caaagcatgg ggtgggccaa
     3481 gtaccatgaa gacaacgtca gccgcttctg gcgggacgcc aatacacagg tgagggtggt
     3541 acacggaact caggaccgtc cggcaagcaa agccggcgct ggcgatgacc tcggcgaact
     3601 caagctgctt cggcgccggc tggcggagaa cccgtgtgac cggagcgccc agcttacgtt
     3661 tcacgcagag aagtcgcgcc tagaaaagct tggcatcagc actggcgaaa tcttgggggt
     3721 cattcacggc gatgaccgcc gagcagatgg accaatggac atcgctagtc cgaatggcgg
     3781 aacgtcgctc gtccagaggc aactgcagaa tgagatcggg cgtaacgctc agccgaacag
     3841 tcccaaggcc ctggcgaagg cccaattgaa taagcaagga gaacggaaca tgtcgaaact
     3901 caaggacttc acagtctcga cggcgcgccc cctacctgtg atcgttctcg ccgacgtcag
     3961 cggcagcatg agcgcgaacg gcaaaatcga tgcgctcaat gacgcgatcc aatcgatgat
     4021 tgagagcttc gcggccgaag atcacagccg tgccgagatc cacgtcgctg tcatcgcatt
     4081 tggcaagggc agcgcaaggc ttcaccaggc tttattgccg gcggcgcaga tcaagtggga
     4141 gcgggtcggc gcagcgggca atacgcccat gggcgcggcg ttcgatctgg tcgtgaagat
     4201 ggtcgaggac caagcgcaga ttccaagccg ggcataccgg ccgacggttg tgctcgtatc
     4261 cgacggacag cccaccgacg agtggcgcga acccctgcag cgcttgctcg cttcggaccg
     4321 cgcttcaaag gcctcacggt ttgtgctcgg gatcggagac gacgcggatc ccgcgatgct
     4381 cgccgagttc ctggctgatc cgagcgcgcg cgcgtactcg gcgcacgagg cccggcagat
     4441 caagaatttc ttccggtggg tcaccatgag cgtcacgcag cgcactcgca gcgtgaatcc
     4501 caacagcgtg gtcgtggtcc acccgactgc tctggacgac tatgacttct gaaggtcaca
     4561 tggcacgcag gagccgtgtg gactacttcg gtgcgagtgt tgccggccct gcccaccggg
     4621 cgctacgaaa gcccaacgag gaccaatggc tcggcgcgtc cggtgccttc ggcagcctgg
     4681 tcgtggtgag cgacggtttg ggctcgaagg accgtgcca
//
LOCUS       DAOQDS010000137         4203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_14536_length_4203_cov_19.242768, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000137 DAOQDS010000000
VERSION     DAOQDS010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14536_length_4203_cov_19.242768"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..>1394)
                     /gene="malQ"
                     /locus_tag="PK589_18420"
     CDS             complement(183..>1394)
                     /gene="malQ"
                     /locus_tag="PK589_18420"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNJ21642.1"
                     /translation="RDRVYHLKQEALQLLWNSFEGDEDFDRFQSAGGRSLRQYALHCC
                     LVERYGSDWRRWPAVYRHPDSAEAEPALPAEVDRITFHMWQQWLLGRQLTDAGQIIPL
                     MQDLPIGFSPDGADAWTWQDVLAAEMTVGAPPDLFNATGQDWGLPPFIPHKLRATGYQ
                     PFIETIRSIICRAGGLRIDHVMGLFRLWWVPRGSPPKDGAFVRYAAEELLAILAVEST
                     RAQAIVVGEDLGTVEDGVRETLAQQNVLSYRLLWFEDQPPSQYPWKALASVTTHDLPT
                     LAGVWTGADLESQRKSGASPDEKAQEQFRTKLVEVSQVEDQTNLEEVITKTFSKLAES
                     PCAVVMADLEAACAVTERPNMPTSGGNYPNWSLALPCSLEELTAAERPAKLAHILNRR
                     VGDTIGTSSRP"
     assembly_gap    1395..1494
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1976..3835
                     /gene="treZ"
                     /locus_tag="PK589_18425"
     CDS             1976..3835
                     /gene="treZ"
                     /locus_tag="PK589_18425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HNJ21643.1"
                     /translation="MSSVRQRCGAHLSADGRMEWRVWAPYAKTVDLIVIDGAARRVEA
                     MTAEPHGYFFIRGGSASHGQQYAYSLDKGPDRADPASRWQPDGVTQPSAIVLPSRFQW
                     TDDRWEGVARDQLVFYEVHVGTFTAEGTFDAIIARLPSLRQLGVTALELMPVGEFPGT
                     RNWGYDGVYPFAPHHSYGGPEGLQRLVNACHEQGLACVLDVVYNHFGPEGSVQYEFGP
                     YFTDHYKTPWGRAVNFDGPGSEGVRAYVIENVRMWISDYHIDGLRLDAVHAIYDFGAT
                     HLLKEIKQAATEAAGDRKFPAYVVAESDLNDVRILLREDQGGYELDAQWSDDFHHTVH
                     ALLTGERQGYYSDYGTAEQLSAVLEKTFAIDGGFSRHRNRCHGAPAAGLPGDRFIGSI
                     QNHDQVGNRAGGERLSHLLPPPLQRLAAAFLLLAPHLPLLFMGEEYGEARPFQFFCSF
                     DGEELIESVRKGRRQEFEAFHTSDAQVTDPQDPSTFDQSRLTWRWDEVPHQAGLRRLY
                     TDLLEARRVWPAMRNYAERMSRLHRGADGAVVLELVRGGTKAAQAHTIQAFFNPTESV
                     QNLPARALPTLLFSSEWERYDGVRASSSESGQLLPFECAIFGPSDWKRYAGLE"
BASE COUNT          815 a         1139 c         1265 g          884 t
ORIGIN      
        1 tcaggtcgaa gagccgatct acattcggcc ggccgagcgc gtgaactggc tgtaaattct
       61 gcctagctga gagcgggtag ccgctctcag ctaggaaggg tgattgctct attgatggag
      121 gccatacacg caacgatgtc tcgcaggcgg cgtgggatcg tggtcgcgat ggcgtgaaga
      181 ggttaggggc gagaagaagt gccgattgtg tcaccgacac gccggttcag tatgtgtgct
      241 agcttcgcgg gccgttcagc cgctgtgagt tcctccaggg aacagggcag ggcaagcgac
      301 cagttcggat agttgccgcc cgaagtgggc atgtttgggc gttcggtcac tgcgcaggcg
      361 gcttcaagat ccgccatcac caccgcgcag ggtgactccg caagtttcga gaacgtcttg
      421 gtgatcacct cctcgagatt cgtctgatcc tctacctggc tcacctccac aagctttgtg
      481 cggaactgct cttgggcctt ttcgtccggt gaggcgccgg atttgcgctg tgactcgagg
      541 tccgcccctg tccagacgcc tgctaaggtg ggaaggtcat gggtggtgac ggaggccaac
      601 gctttccagg ggtattgcga cgggggctga tcttcgaacc aaagcaggcg ataggacaag
      661 acattctgtt gggcgagggt ctctcgtaca ccgtcctcca ccgtcccgag gtcttctcct
      721 acgacgatgg cctgcgcgcg agtgctttcc acggcgagga tcgccaggag ctcctctgcc
      781 gcataccgca cgaaggcacc gtccttgggc ggactgcctc gagggaccca ccacaggcga
      841 aagagcccca tcacgtgatc gatgcgtaag ccgccggcac ggcagatgat ggagcggatc
      901 gtttcgatga acggttggta gccagtcgcc cggagtttat gcggaatgaa tgggggaagt
      961 ccccaatcct ggcccgtcgc attgaagaga tcaggcggcg ctccgaccgt catctcggct
     1021 gcaagcacgt cttgccatgt ccaggcgtcg gcgccgtccg gtgaaaatcc gatgggtaga
     1081 tcctgcatca aggggatgat ctgccccgcg tcggtgagtt gtcggccgag cagccactgt
     1141 tgccacatat gaaatgtgat gcggtcgact tctgcgggca gggccggttc ggcctcggct
     1201 gagtccgggt gacgatagac ggcaggccag cgacgccagt cggagccgta acgttcaacc
     1261 agacaacagt gaagtgcata ctggcgcagc gagcgccccc ctgcggactg aaagcggtcg
     1321 aaatcttcgt ccccttcaaa gctattccac aacagttgta aggcctcttg cttgagatga
     1381 tagacgcgat cgcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgaatg
     1501 ccccatgcat cggtcttcat gcctgatggt ctccttggct cttcctctcc tgtccggttc
     1561 gtgccgtcac tcaatcacgg tctccttgcg ctgtcttcct gccaggcctt gggccatggc
     1621 ctaaagcgtc cgtgaggtaa aggtgcaacg ccacgtctgc ggacgatgat gtcgttggtt
     1681 gtaggacggc gctgcgttat gcaatgtcca ggctcggctt gtgggcgagt gattccacgg
     1741 gattttatga gagaaaagat cagatcgcga cgtatcgcgg tggccttttg tccctagaca
     1801 gacaacttgt ccccgttgtt cggaccctca ttctcctaaa agtgagacca ggcaagagcc
     1861 gtcagcggag gagagagacg ccgtgtgtgg cgcatgcctt gcacagggct acccaacctg
     1921 tagcgttctt cattcatgta ggtagtttcc gaacatagga tgtggaggag atgggatgag
     1981 cagtgtaagg cagagatgcg gcgcgcatct gtcggccgat gggagaatgg aatggcgtgt
     2041 ctgggctcct tacgcaaaga cggtcgatct catcgtgatt gacggcgcgg ctcggcgtgt
     2101 agaagcgatg accgccgaac cgcatggata ctttttcatc cgtggaggca gtgcgtccca
     2161 cgggcagcaa tatgcgtatt ccctcgataa ggggcccgac cgtgcggacc ctgcatcacg
     2221 ctggcaaccg gatggggtaa cgcagccgtc ggctatcgtt ttgccttcgc gctttcagtg
     2281 gacagatgac cggtgggaag gagttgcgcg ggaccagctc gtcttctatg aagtgcatgt
     2341 cggaaccttc acggctgaag gaacgtttga tgccatcatt gcccgccttc cctcattgcg
     2401 gcaactcggt gtcactgctc tggagctgat gcctgtgggg gaattcccgg gcaccagaaa
     2461 ctggggatac gacggggtct atccgttcgc gcctcaccac agttacggcg ggcctgaagg
     2521 cctccaacgg ctcgtcaacg cctgccacga acagggcctg gcttgcgtgc tggacgtggt
     2581 ctataaccat tttggccccg agggcagcgt tcaatatgag ttcggcccct atttcactga
     2641 tcactacaag acgccatggg gacgcgcggt gaattttgac gggcctggaa gcgagggggt
     2701 gcgtgcctat gtcatcgaga atgttcgcat gtggatcagc gattaccata ttgatggatt
     2761 aaggctggat gccgtgcacg cgatctacga tttcggcgcg acgcaccttc tcaaagaaat
     2821 caaacaagcc gctaccgagg ctgccgggga cagaaaattc ccggcgtacg ttgtcgcgga
     2881 aagtgatctg aacgatgtgc gtatcctttt acgggaggat caaggcggct acgagctgga
     2941 tgcgcaatgg agcgatgact ttcatcacac ggtccatgcg ctgttgacgg gagagcggca
     3001 gggctattac tcggactatg gcacagccga acagttgagt gcggtgctag agaaaacgtt
     3061 cgccatcgat ggaggtttta gccgtcatcg aaaccgatgt cacggcgcgc ccgcggcggg
     3121 ccttcccgga gatcgattca tcgggtccat acaaaaccat gatcaggtag gtaatcgagc
     3181 aggtggcgag cggctgagtc atctgctccc accgcctctc cagcggcttg ccgcagcgtt
     3241 cttgctactc gccccacacc tcccgttgct gttcatgggg gaagagtacg gcgaagcacg
     3301 accctttcag tttttttgct ccttcgacgg ggaggagctg atcgagtctg tgcgcaaggg
     3361 gcgacgccag gaatttgaag ccttccatac gagtgacgcc caggtgacgg atccccagga
     3421 cccatctacg tttgaccagt cccgtcttac gtggcggtgg gatgaggtcc cccatcaagc
     3481 gggattgaga cggctctata cggatctact ggaagcccgc cgagtctggc cggccatgcg
     3541 aaattacgcg gagcgcatga gcaggctcca tcgtggagct gacggggcag tggtgttgga
     3601 gttggtgcgg ggagggacca aagcagcgca agcacacacc atccaagcgt tctttaatcc
     3661 tactgagagc gttcagaatc tgccggcccg ggcattacca accttgttgt ttagttcgga
     3721 atgggaacgc tacgatggcg ttcgagcgtc atcttctgag agcggtcagc tgctcccgtt
     3781 cgaatgtgcc atcttcggtc cctctgattg gaaacggtac gcagggcttg agtgaggact
     3841 gggcggcgac agctaactag gtagctaaac atctacgaat ccctgccgag agtcattcag
     3901 agctcgaggc agtcctacgt atatctcgcc gcattcgcac accactcgac gttgctcaat
     3961 gcgagcgtct ctgagccgca gaacagtccc gcgcaccctg cgcaggcggc ccatatcctg
     4021 ttctttcata aaatttaggc tgtcaatcat tgctgacagt gctacagggt tcgccccagt
     4081 tatcaacgaa gcgtccttct cctaggatag gcccacgtga cactccttgt acgtagcgct
     4141 caggccggat acgttcatgt cctcgccagt cgctcgtggc agggctattt caactcatgt
     4201 ggc
//
LOCUS       DAOQDS010000138         4145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_14879_length_4145_cov_20.222249, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000138 DAOQDS010000000
VERSION     DAOQDS010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14879_length_4145_cov_20.222249"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..362
                     /locus_tag="PK589_18430"
     CDS             36..362
                     /locus_tag="PK589_18430"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HNJ21644.1"
                     /translation="MKKVTVLFSLSLISAAYLSLTGCQIVASPMAGGIYNETKYGDVA
                     TTHNSATKEGKACGTSILGWVATGDASVSAAKAAGGITTVASVDHSAKNILGILGEWC
                     TIVKGS"
     gene            459..1091
                     /locus_tag="PK589_18435"
     CDS             459..1091
                     /locus_tag="PK589_18435"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ21645.1"
                     /translation="MWVPLFVIILSASFLFPLSAGCAEFGEIDLAGYLMGSWPRDQPI
                     FNQGSMVTASIQSGVGAGVKIGLFPHAMHRMLGIEIDSYGHGGALSFPNMANGQNNGT
                     GRSNLLVLNTMLNLILRYPGEVVTPYIGIGGGWSHGTLLNPNITGRSDKDFDSARALG
                     YQYLAGAQILVSPKVFIFGEYRYFSANYHWDSLAMDFRAHYGLFGVGLRF"
     gene            1098..1859
                     /locus_tag="PK589_18440"
     CDS             1098..1859
                     /locus_tag="PK589_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ21646.1"
                     /translation="MSLLDQFFVYHPHPWEERDWSAVGGVPLEDIWFQAIDGTKLFGW
                     YAENPATSAVLLWCHGNAGNMTHRLENLAALYRLGLSVFLFDYRGYGKSHGRPSEDGL
                     YRDALGAYDYLTKVRRVRPERVVIFGRSLGGAVAGELATQRAAMGLLLESCFPSIEAV
                     ARHHYMGLPMHWLLGAAFRLEDRLPHLSLPKLFVHGDRDDIIPVELGQQAFAAAKAPK
                     EWYVVRGADHNDVPSVGGRAYFAKLFAFIHAAIGR"
     gene            2118..3068
                     /locus_tag="PK589_18445"
     CDS             2118..3068
                     /locus_tag="PK589_18445"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNJ21647.1"
                     /translation="MEVVSAVLVLFVVGCAGGPAQTTRQAGSGMESVAQGELRTEGTN
                     PVQVAASPAVSSNMVVLVANKAASPAAEKEPADQPEEDPFFDPFAKSDEPAGGEEYDP
                     WEPVNTKIFEFNRQVDRWVLKPAARGYNAVVPNPVQIGISNLFYNMRFPSRLINNLAQ
                     GKFSGAGTEVGRFLLNSSFGLGGLVDVAKYMDITTPEEDTGQTLGYYGVKPGPYVVLP
                     FLPPFTLRDLVGYVGDIALNPINWLVFPIIEINGVPSLVAHHNRTTSSIAQISGRVEE
                     ILNDRSLNLEKFQGVEEATLDLYTAVKNAYIQKRRNAIRE"
     gene            complement(3072..>4145)
                     /locus_tag="PK589_18450"
     CDS             complement(3072..>4145)
                     /locus_tag="PK589_18450"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNJ21648.1"
                     /translation="AQGEVVNLCANNYLGLANHPDVRRAAADGLKDYGYGMASVRFIC
                     GTQDLHKRLEQAISTFFGTADTILYSSCFDANGGLFETLLDERDTIISDALNHASLID
                     GIRLCKASRLRYAHADMEDLEARLAESSASRVRMIVTDGVFSMDGDLAKLDRIADLAD
                     RYDAAVVVDDSHATGVLGKGGRGTPDHFGVAERIDLVTSTLGKALGGATGGFTTGRKE
                     LIELLRQRSRPYLFSNSLPPVIAAAALKAIALVEQGDHLRMNLMEQAGWLRSQLAALG
                     FTLVPGHHPIIPVMLGDASIATKMADRLLQEGIYVVGFSYPVVPKGQARIRLQLSAAH
                     TRAQLERAVAAFATVGRELHVIN"
BASE COUNT          828 a         1144 c         1262 g          911 t
ORIGIN      
        1 gcaccatcaa ccgctaactt cacgagggag gacttatgaa gaaggtgacc gtgttgtttt
       61 ctctgagcct gatcagcgca gcgtatctca gcctcaccgg ttgtcagatc gtggcgtccc
      121 cgatggcggg tggaatctac aacgaaacga agtatggcga tgtggccacg acccacaaca
      181 gcgcaaccaa ggaagggaag gcctgcggca cgtccatcct cggttgggtg gcgacgggtg
      241 atgccagcgt ctcggcggcg aaagcggccg gcggaatcac cacggtggcg tcagtggatc
      301 actcggcgaa gaacatcctc ggcatccttg gcgaatggtg cacgatcgtt aagggcagct
      361 gatcacgctt gttgtccctc tgaagaaagg gtcttcggga taagctcccg aagacccttt
      421 ttcttttcct tcacccacca tggtacgcta cgacgcgtat gtgggttccc ctcttcgtca
      481 ttattctctc cgcttcgttt ctgttcccac tgtcagctgg gtgtgccgaa ttcggtgaaa
      541 tcgacctggc tggttatctg atggggagct ggccgcgtga ccagccgata tttaaccagg
      601 gcagcatggt gacggcgtcc attcaatcag gggttggcgc aggtgtaaaa atcggtttgt
      661 ttcctcatgc gatgcaccgg atgctcggca tcgaaattga ttcgtatggc cacgggggag
      721 ccctatcgtt tcccaacatg gcgaatgggc agaacaacgg gaccggtcgc tccaacctcc
      781 ttgtgctgaa cacgatgctc aacctgatcc tccgctatcc cggtgaggtg gtcacgcctt
      841 acatcggcat cggcggcggc tggtcgcatg gcacgctgct caatcccaat atcacaggcc
      901 ggtccgataa ggatttcgat tcggcgcgcg cgctgggcta ccagtatctg gccggcgcgc
      961 agatcttggt gagccccaag gtgttcatct ttggtgagta tcggtacttt tcggcgaatt
     1021 atcattggga ttctctggcg atggattttc gcgcccatta cgggctcttc ggagttggat
     1081 tgcgtttcta acgtggcatg agcctgctgg accaattttt tgtctatcac ccgcatccgt
     1141 gggaagaacg cgactggtct gccgtcggcg gggtgccgtt ggaggacatt tggtttcagg
     1201 ccatcgatgg cacgaaactc ttcggctggt atgccgagaa tcccgccacc tctgccgtgt
     1261 tgctgtggtg tcatggtaat gccggcaata tgacccatcg attggagaat cttgccgcgc
     1321 tctatcgcct gggattgtcg gtgtttctat tcgactatcg aggttatgga aaaagccacg
     1381 ggcgcccgtc ggaagacggt ctctatcggg atgcactggg cgcctacgac tacctgacga
     1441 aagtgcgtcg agtcaggccg gaacgggtgg tcatcttcgg acgctcattg gggggcgcgg
     1501 tagccggaga gctggcgacg caacgcgcgg ctatggggct cttgctggaa tcctgctttc
     1561 cttcaatcga agcggttgcg cgtcaccatt atatggggtt gcccatgcat tggttgctgg
     1621 gcgccgcgtt tcgattggaa gaccgtctgc cgcatctttc gttgccgaag ttatttgtgc
     1681 atggcgaccg cgacgacatc attccggtgg aactcggcca acaggcgttc gccgctgcca
     1741 aagctcccaa agagtggtat gtggtgcgcg gagccgatca caacgatgtg ccgtcggtcg
     1801 gagggcgagc ctattttgcc aagctgttcg ccttcattca cgctgccatt ggtcgttgac
     1861 ctggtaaaaa atctccgcat cttctgtatt ttttctaggc agattcagct cgcttggtaa
     1921 gatgcgatgg agcggtcagg cataaggcca ggagatcggc atcagagggc gtcctcatct
     1981 ggcagctgtc cgtatattct gctagagtgc gtccgcctgc gcgccctgaa gacgcgcggt
     2041 ggcaacgttc aatcagcgtg aaaagccctc ggcgaggagg caaggtgcat cggacaggag
     2101 tgcatgcaag aaggttcgtg gaggtggtgt ctgcagtgtt ggtcctcttc gtagtgggat
     2161 gtgccggcgg ccctgctcaa actacgcgac aggccggtag tggaatggaa tcggtcgccc
     2221 aaggagagtt acgaaccgaa gggacgaatc cggttcaggt cgccgcgtct ccagcggtct
     2281 cgtccaacat ggttgtgttg gtggccaata aggccgcttc gcctgcagcc gagaaggaac
     2341 cggcagacca acctgaagag gatccgtttt tcgacccctt tgccaagagc gacgagcctg
     2401 cagggggtga agagtatgat ccttgggaac cggtgaatac caaaatcttc gagttcaatc
     2461 ggcaggttga ccggtgggtg ctcaagccgg ctgcgcgagg atacaatgcg gtggtaccga
     2521 acccggtgca gatcgggatc agtaacctct tttacaacat gcggtttcct tcccgcctga
     2581 tcaacaatct tgcgcagggg aaattctccg gtgcaggcac cgaggtcggc cggtttctcc
     2641 tgaatagcag cttcggattg ggcgggctgg tggatgtggc gaagtatatg gatatcacca
     2701 cgccggaaga agataccggt cagacgctgg gctattacgg ggtgaagccg gggccgtacg
     2761 tcgtattgcc atttctgccg ccattcacgt tgcgcgatct cgttggctat gtcggagaca
     2821 ttgcgctcaa tccgatcaat tggctggtct ttcccatcat tgaaataaac ggcgttcctt
     2881 cgctggtggc ccatcacaat cggaccacgt cgtcgatcgc tcaaatcagc ggccgcgtgg
     2941 aagaaattct gaatgaccgc tcgttgaacc tggagaagtt tcaaggcgtg gaggaagcga
     3001 ctctggattt gtacacagcg gtgaaaaacg cctacatcca gaagcggcgc aacgccatca
     3061 gggaatagcg actagttgat gacgtggagc tcgcgcccca cggtggcgaa ggcggcgacg
     3121 gcccgttcga gttgggcgcg ggtgtgggcc gcggagagtt gcaggcgaat acgcgcctgt
     3181 ccttttggta cgaccggata gctgaatccc accacataga ttccttcctg cagcaggcgg
     3241 tcggccattt ttgtggcgat cgaggcgtct cccagcatga ccggaatgat gggatgatgg
     3301 cccggtacga gggtgaagcc aagcgcagcc agctggctcc gcagccagcc ggcctgctcc
     3361 atcaagttca tgcgtaaatg gtcgccttgc tcgaccaagg caatagcttt caacgcggcg
     3421 gccgcgatga ccggcggcag actgttcgaa aacaagtagg gacgcgaccg ttgccgtaac
     3481 agctcaatca attccttcct gccggtggta aacccgcccg ttgcgccacc gagcgccttg
     3541 cctagggtgc tggtgacaag gtcgatccgc tcagcaacac caaaatgatc aggggtgccg
     3601 cgaccgcctt tccccaagac cccggtcgca tggctgtcgt ccacaaccac ggctgcgtca
     3661 tagcggtcgg ccagatcggc aatgcgatcc agtttcgcca ggtcgccgtc cattgaaaat
     3721 accccgtccg tgacaatcat tcgcacccgg ctcgcagagg attccgctag tcgcgcttcc
     3781 aggtcctcca tgtcggcatg ggcatagcgc aggcgtgaag ccttgcagag acgaatgccg
     3841 tcgatcaggc tggcatggtt cagggcatcg ctaatgatgg tgtcccgctc atcgagcaat
     3901 gtctcgaaca atccgccgtt ggcgtcgaag caggagctgt agagaatggt gtccgcggtg
     3961 ccgaaaaagg tgctaatggc ctgctcgagc cgtttgtgca agtcctgcgt cccgcaaata
     4021 aaacgcaccg aggccatacc gtatccgtag tccttcagcc catccgccgc cgctcgccga
     4081 acatcaggat ggttggccag cccaagatag ttattcgcac agaggttgac gacctcaccc
     4141 tgggc
//
LOCUS       DAOQDS010000139         4009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_15736_length_4009_cov_19.051593, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000139 DAOQDS010000000
VERSION     DAOQDS010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15736_length_4009_cov_19.051593"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1992)
                     /locus_tag="PK589_18455"
     CDS             complement(<1..1992)
                     /locus_tag="PK589_18455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ21649.1"
                     /translation="MTDIQAKILLVDDDARSLMAMETLLTGPGRVVITADSGHEALRQ
                     VLRHDFAVILLDVRMPRVDGFEAAELIRQREQSRHTPIIFLSAVDKLEEDVFRGLASG
                     AVDYLFKPVVPAVLQSKVSVFVDLYRLRERVKLQAVRQGEERFRLLVDSIRDCSILLL
                     SADGAVTSWNPGTRLVEGYEADDVIGRNHSLFCTAEDIARGYPGLALDTAAAAGQHEA
                     EGWRVRKDGSTFWAHVVITAMKDETGKLSGFARVARDLTERKVAEEQLRKIAVELEQR
                     VSERTLELRESQMRLRELATELTLTEQRERRRLAGDLHDYLAQLLVLIRIKIHQISAQ
                     VKEPAPASLLMEADHAIIDALDYTRSLVAELAPPALQEFGLLEGFEWLAEQMKNHGLK
                     VTVHKHVQDVVLGADQAVLVFQSTRELLFNVLKHAGTNQATLIIDRNSDGHLVVSVKD
                     EGRGFDPHAMLARNDERKRFGLFSVRERTEAMGGKLEVTSLSNQGTCAALIVPYWPIE
                     ANQSTAPDSAVSPARSQGAPSFLSSEPANGERTNDATGESTQAGVLRIVLADDHVMVR
                     QGLRSILDSYPDISIVGEAADGEEAVELAGRLAPDVIVMDVNMPKMDGIEATRRIKAR
                     WPGITVVGLSVSTAAQVESLLLEAGASCYVSKEAAGIHLY"
     gene            complement(2069..>2517)
                     /locus_tag="PK589_18460"
     CDS             complement(2069..>2517)
                     /locus_tag="PK589_18460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNJ21650.1"
                     /translation="ADKQRMLERVRQSDDALVGKKVLVVDDDMRNIFALTSLLERHGM
                     EVTGCETGREAIQQVEEDPDLDMVLMDIMMPEMDGYETMRHIRQNPQHRLLPILALTA
                     KAMKGDRERCLQAGASDYIAKPVNTDELLALLRIWLFGRKKAMGKP"
     assembly_gap    2518..2617
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2618..>4009)
                     /locus_tag="PK589_18465"
     CDS             complement(<2618..>4009)
                     /locus_tag="PK589_18465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ21651.1"
                     /translation="QQLAEQNAEVERKNREIEQARHALEDKARELALASKYKSEFLAN
                     MSHELRTPLNSILILAQQLSENPDQNLSSKQVEFSKNIHAAGADLLNLISDILDLSKI
                     ESGTVTVEPEEVLFMKVREATERSFRHVAEARQLAFHIEIDPQLSRSMVTDFKRLQQV
                     LKNLLSNAFKFTSHGQVTFRIRRAVTGWSLDHAVLSTVPTVVAFEVSDTGIGIPVEKQ
                     KIVFEAFQQADAGTSRRFGGTGLGLAISRELAMLLGGEIRLVSAPGEGSTFTLYLPEA
                     YMGATPEMISPKFQMQEQTPLLVQQAHRLEDIPDDRETILPGDPTVLIVEDDPHYARV
                     LLGAVREKGLKGIVTAHGRLALALTREYKPVAITLDIFLPDMLGWTVLSHLKQDPETR
                     HIPVQILTVEEERMHGLGHGAFAYMIKPATTEDLGETFGRLVEFTKPRRKQLLLVEDN
                     EIESRSLCELLRHE"
BASE COUNT          768 a         1221 c         1068 g          852 t
ORIGIN      
        1 gtagagatgg atgccggcgg cttccttgga gacataacag ctggctcccg cttccagcag
       61 caacgattca acctgcgcgg ccgtcgagac ggagaggccc acaacggtga tccccggcca
      121 tctcgctttg attcgacgcg tggcttcgat gccatccatc ttcggcatat tcacatccat
      181 cacgattaca tcgggagcca accgaccggc caattctacg gcttcttccc catctgccgc
      241 ctcgcccacg atggagatgt cgggatagct gtccaaaata ctgcgcagcc cttgccgcac
      301 catgacatgg tcatcggcga ggacgatgcg caggaccccg gcttgcgttg actcaccggt
      361 tgcatcattg gtgcgctcgc cgttagcagg ctcggaagag agaaacgatg gcgccccttg
      421 agatcgcgcc ggcgacacgg ccgagtcagg cgccgtgctt tggttggctt caatgggcca
      481 gtagggaacg atcaatgcgg cgcaggttcc ctggttggac aaggaagtga cttccagctt
      541 gcctcccatc gcttcggttc gttcccgcac actgaataag ccgaatcgtt tccgctcatc
      601 gttccgggcc agcatggcat ggggatcaaa cccccgcccc tcatccttca cgctcacgac
      661 cagatggccg tcgctgtttc gatcaatgat cagggtcgcc tgattcgtcc cggcatgttt
      721 gagcacgttg aacagcaatt cacgcgtgga ttgaaacacc agcacggcct gatccgcccc
      781 gagaaccaca tcttgaacgt gtttgtggac ggtgaccttt aagccgtgat ttttcatctg
      841 ctccgccaac cactcaaagc cttcgagcag accaaactcc tggagcgccg gaggagccag
      901 ttctgcgacc agcgatcgcg tgtaatccag tgcgtcaatg atggcatggt ccgcttccat
      961 caatagggat gccggcgccg gctccttaac ctgggcggag atctggtgga tcttaattct
     1021 gatcaatacg agcagctgcg caagataatc gtgcagatcc ccggccagcc ggcggcgctc
     1081 acgctgttcc gtaagggtca attcagtcgc cagttcacgg agccgcattt gagattcgcg
     1141 gagctccagc gttcgctcgc ttacgcgctg ttccagctca acggcgatct ttcggagttg
     1201 ttcttcggcc actttgcgtt ccgtgaggtc ccgcgccacg cgtgcaaagc cgctgagttt
     1261 ccccgtctca tccttcatgg cagtaatgac gacgtgggcc cagaatgtgg agccgtcctt
     1321 ccgcactcgc cacccttcgg cttcatgttg accggcagcc gcagccgtgt ccaacgccag
     1381 gcccgggtag cctcgcgcga tatcttcggc cgtacaaaag agcgaatggt ttcggccgat
     1441 cacatcgtcc gcttcatagc cttccaccag gcgggtccct ggattccagc ttgtcaccgc
     1501 cccgtctgcg ctgaggagga gaatcgagca atccctgatg ctatccacca gcagacgaaa
     1561 ccgctcctcg ccctgccgta cggcctgcag tttcacgcgc tcgcgcaggc gatagaggtc
     1621 gacgaacacc gacaccttgc tttgcagcac agcggggacc acgggtttga acaggtaatc
     1681 gactgctccg ctggccagcc ccctgaacac gtcctcctcc agcttatcga cagccgagag
     1741 aaagatgatc ggcgtatggc gcgactgctc gcgttgtcgg atcaactcgg cggcttcgaa
     1801 tccatcaacc cgcggcatac ggacgtcgag gaggatgacg gcaaaatcgt gccgcaagac
     1861 ctggcgaagc gcctcgtgcc cggaatccgc cgtgatcacg acacggccag gtcccgtcaa
     1921 caaggtctcc atggccatca ggcttctggc atcgtcatcc actaagagga tcttggcctg
     1981 aatgtcggtc ataaaggact cctcttcaac gcagctcagt gttgccggca tggggatgtg
     2041 gcacgaacag caatcggcca gaactccgtc atggtttgcc catggctttt ttgcgtccga
     2101 agagccaaat cctcagaagg gccagcaact catcggtatt gaccggtttc gcaatataat
     2161 ccgaagcgcc cgcctgaagg cagcgttcac gatctccctt catcgccttc gcggttaagg
     2221 ccagaatcgg cagcaatcga tgctgagggt tttgccggat gtggcgcatg gtctcatagc
     2281 cgtccatttc aggcatcatg atgtccatca gaaccatgtc cagatcagga tcctcttcca
     2341 cctgctgaat ggcttcacgg ccggtctcac acccggtcac ctccatgcca tgccgctcga
     2401 gcaggctggt cagggcgaaa atatttcgca tatcgtcgtc caccaccagg actttcttgc
     2461 ccaccagcgc gtcatcgctt tgccgcaccc gttccaacat gcgctgtttg tccgccgnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttc gtgccggaga agttcacaca
     2641 ggctgcggct ttcgatttca ttgtcctcga cgagcaacaa ttgcttgcgc ctgggtttgg
     2701 tgaactcgac cagccggcca aaggtctccc cgagatcttc cgtcgtggcc ggtttaatca
     2761 tgtaggcgaa ggcgccatgt cccagcccgt gcattcgttc ctcttccacc gtgaggatct
     2821 gcacaggaat atgcctggtc tcaggatcct gcttgaggtg gctcaagacg gtccacccga
     2881 gcatgtccgg gagaaagata tccagcgtga tggccaccgg cttgtactct ctggtcaggg
     2941 ccaaggccag gcggccatgg gcggtgacaa tccctttcaa ccccttttcc cggacggccc
     3001 cgaggagcac gcgggcataa tgcggatcat cttccacgat cagcacggtt ggatcgccgg
     3061 ggagaatcgt ttcccgatca tcgggtatgt cctccagtcg atgggcctgc tgcaccagca
     3121 acggcgtctg ttcctgcatc tgaaatttcg gagagatcat ttccggcgtc gcgcccatat
     3181 aggcttcagg cagatacagc gtaaaggtgc ttccttcgcc gggggcgctc accaaccgga
     3241 tttctccgcc taacagcatg gccaattcgc ggctgatggc gaggccgagt cccgtaccgc
     3301 caaaccgccg gctggttcca gcatccgcct gttggaacgc ctcgaacacg atcttctgct
     3361 tctccaccgg aatgccgatg cccgtatcac tcacttcaaa cgccaccacg gtcgggaccg
     3421 tgctcaagac cgcatggtcc agactccagc cggtgacggc gcgccggatc ctgaaggtga
     3481 cttgaccgtg agacgtaaac ttgaacgcat tggacaggag atttttcaac acctgctgca
     3541 atcgcttgaa gtcagttacc atacttcgtg ataactgcgg gtcgatctcg atgtgaaagg
     3601 cgagctgccg cgcttctgcg acatgccgga aactgcgctc cgtggcctcc cgcaccttca
     3661 tgaagagcac ttcttccggt tcgaccgtca ccgtgccgga ttcgatcttc gaaagatcca
     3721 agatatcgct gatcaggttg agcagatccg cgccggcggc gtgaatgttc ttggaaaact
     3781 cgacttgttt ggagctgaga ttttggtccg gattctcgct aagctgttgc gccagaatca
     3841 ggatgctgtt gagaggtgtc cgcaactcgt gggacatatt ggcgaggaat tcggatttgt
     3901 atttcgaggc caaggccaac tcacgcgcct tatcctccag cgcatgtctg gcctgttcga
     3961 tctcccgatt ctttcgttcg acctccgcat tctgctcggc cagttgctg
//
LOCUS       DAOQDS010000140         3781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_17428_length_3781_cov_22.914385, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000140 DAOQDS010000000
VERSION     DAOQDS010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17428_length_3781_cov_22.914385"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..527
                     /gene="nrdR"
                     /locus_tag="PK589_18470"
     CDS             63..527
                     /gene="nrdR"
                     /locus_tag="PK589_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNJ21652.1"
                     /translation="MKCPFCDDVEDKVVDSRMAKEGEVIRRRRECLSCKRRYTTYERV
                     EETMPAVVKKDGRREPFDRSKIVSGLKKACEKRPISTATIETVTDRIEKRIQDMGETE
                     IVSTAVGEEVMKELSQLDQVAYVRFASVYREFKDIDQFMDEIKSLAQQRRER"
     gene            635..1756
                     /locus_tag="PK589_18475"
     CDS             635..1756
                     /locus_tag="PK589_18475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNJ21653.1"
                     /translation="MATTKTSQERRTTRRPTNRRSPGATHVVIIGAGRGGTALMEIFA
                     NDPLVRIVGVADISPEAPGLGLAKRLHIRVTRNYRQLLKMGPVDLVIDVSGNPEVGEY
                     LQDIRRMGVSVIGGASAKFMWQLIEARIRATADIEKALNKYQSLYRLYVKETGAAVTE
                     ERTRIACEIHDGLVQSLAGVNFKLELSQELLRKNPQASLATIRESKAQLKLAIQEARQ
                     VIFNLRPLHYDKMELIPALTNYLKSYETQYHIKTAFSVTGDETILFPRTKIFLFRIVQ
                     EALSNVQKHAKAGRVSVQLDIRLDLLQVTISDNGIGFDMDAVLRDPEKWDHFGIRGIL
                     ERARLVGGEATIDSKKGRGTRIVLRIPLADKETIRHGKN"
     gene            1743..2414
                     /locus_tag="PK589_18480"
     CDS             1743..2414
                     /locus_tag="PK589_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ21654.1"
                     /translation="MEKIKVLIADDHRVVREGLAAILKTKEDINVVGEAQDGVEAVEK
                     TKTLMPDVILMDVSMPRMGGIEATRQIKREFPHMGIVALTMYEEQQYIFDLVRAGATG
                     YLLKDSESSQIVAAIRAIYRGESLIHPSVASKILAEFSLMSQKKGKKPAWVEHDLTER
                     EITVLRLVADGKTNKEIANSLDLSEKTVKNHVRNIFHKLQVYDRTQAAILAIRKGLIE
                     LEPRP"
     gene            2700..3626
                     /gene="lpxC"
                     /locus_tag="PK589_18485"
     CDS             2700..3626
                     /gene="lpxC"
                     /locus_tag="PK589_18485"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNJ21655.1"
                     /translation="MRFQQTIGSPVSCSGVGLHSGQPVTLTLRPAPPNTGIVFIYRHD
                     PEETLIPASISNKVPTELCTAISVNGHQVKTIEHLLAALVGMEVDNVYAEVNAGEVPV
                     LDGSSSPFVRLIRAAGVIPQTRRQSYVKIMQPIEVVDGSKRVRIEPSSTPKITYSIHY
                     DHPLIQTQTYTYTCSAQAFEQDIAQARTFGFLHEVEALWSRGLGKGGTLDNTIVLSKE
                     GVVNESGLRFSNEFVRHKVLDLIGDIALLGFPFIGHVVAERSGHAMHTKLVEQILLQR
                     DKWALITGEHAVAASESRPSLGLLRPAPSLAI"
BASE COUNT          919 a         1085 c         1005 g          772 t
ORIGIN      
        1 gcaaccgttt ccccatcttt catgcctacg attcgtctcc cgcttaggca ggacggccgc
       61 ccgtgaaatg tcctttctgc gacgatgtcg aagacaaagt cgtcgattcg cggatggcca
      121 aagaaggcga ggtcatccgc cggcggcgtg aatgcctctc gtgcaaacgc cgttacacta
      181 cctacgaacg tgtcgaagag accatgcccg ccgtcgtgaa aaaggacggc cgccgggaac
      241 cattcgatcg aagcaagatc gtgtccggac tcaaaaaggc ctgtgaaaag cgaccgatca
      301 gcaccgcgac catcgaaacc gtcaccgacc gcatcgaaaa acgcatccag gatatgggcg
      361 aaaccgagat cgtcagcacc gccgtcggcg aagaagtgat gaaagagctg tctcaactcg
      421 atcaggtggc ctacgttcga ttcgcgtcgg tgtaccgaga gtttaaagac atcgatcagt
      481 tcatggatga aatcaaatcc ctggcccagc agcgccggga gcgttaggcc ctcccctcct
      541 tcccgctgtt ccggatcaga gtccgtgtga agagacccag gcaggacagg tgccaacaga
      601 ggagaaccgc cgtccatcgg attgtctgac tgatatggcc acaaccaaaa catcacaaga
      661 acgacgaacg acccgccgcc cgaccaaccg gcgatcaccg ggcgctacgc acgtcgtcat
      721 catcggcgcg gggcgcgggg gcacggcctt gatggaaatt ttcgccaacg acccgctggt
      781 acgcatcgtc ggcgtcgctg atatcagtcc ggaagcgccc gggctggggc tcgccaaacg
      841 ccttcatatt cgcgtcaccc gcaattatcg gcaattgctc aaaatgggcc ccgtggatct
      901 ggtgatcgat gtgtcgggca accccgaggt tggcgaatat ctccaggaca tccgtcgcat
      961 gggcgtttca gtcatcggcg gagccagcgc caaattcatg tggcaactga tcgaagcccg
     1021 catccgcgcc acggccgata ttgagaaagc cttgaacaaa taccaatcgt tgtaccggct
     1081 ttacgtgaaa gagaccggcg cggcggtgac agaagaacga acacgcatcg cgtgcgagat
     1141 tcacgatggc ctggtgcaaa gcctcgccgg cgtcaatttc aagttggagc tctcacagga
     1201 actgcttcgc aaaaacccgc aagccagttt ggcgacgatc cgtgaatcca aagctcagct
     1261 gaagctagcg attcaagaag cgcggcaggt catcttcaat ctacggccgc tccactacga
     1321 taaaatggag ctcatcccgg ccctcacgaa ctatttgaag tcctacgaaa cgcagtacca
     1381 tatcaagaca gccttttctg tgaccggaga cgagacgatc ctgttcccgc gcacgaagat
     1441 tttcttattc cggatcgtcc aagaagcgtt gagcaacgtg cagaagcacg ccaaggcggg
     1501 ccgggtgtcg gttcaactcg acattcgcct cgacctgtta caagtgacca tttccgataa
     1561 cgggatcggg ttcgacatgg acgcagtgct ccgcgatcct gaaaaatggg accacttcgg
     1621 cattcgtggc atcttggaac gagcgcgact ggtgggcggt gaggccacga tcgattcgaa
     1681 aaagggacgc ggcacgcgga ttgtgttgcg catcccgcta gccgacaagg agacgattcg
     1741 tcatggaaaa aattaaggtc ctgatcgccg atgatcaccg cgtagttcgt gaagggctgg
     1801 cggctattct gaagacgaag gaagacatca acgtcgtcgg cgaagcccaa gacggtgtcg
     1861 aagccgtaga aaaaaccaag accctcatgc cggatgtcat cctcatggat gtcagcatgc
     1921 cgcgtatggg aggcatcgaa gccacgcggc agatcaaacg tgaattcccg cacatgggaa
     1981 tcgttgcact gaccatgtat gaagagcagc aatatatttt cgatctcgta cgggcggggg
     2041 caacgggata tctgctgaaa gattccgaat cgtctcaaat tgtggcagca atccgagcca
     2101 tttatcgcgg ggaatcgctc atccatcctt cggtcgccag caagatcctg gcggagtttt
     2161 cactcatgtc tcagaaaaag ggcaagaagc cggcctgggt tgaacatgac ctgaccgaac
     2221 gggaaatcac cgtcctccgc ttggtcgcag acggaaaaac gaataaggaa attgccaaca
     2281 gcctggacct cagtgaaaag acggtcaaga accacgtccg caacattttt cacaaacttc
     2341 aggtgtatga ccgcacgcaa gccgcgattt tggcgattcg aaagggactc attgagttgg
     2401 agccaagacc ctaaggaggt gctcgcttat cccgagtact ggggctgtct ctcccggagt
     2461 tcgtggaccc ttcgttctac gttgcccgca tctttcagga cacacagccg ataactcttt
     2521 gaaaaagcgt tactttcgca ccttttcatt ttcaacaaaa gatacaattg tgttttttta
     2581 gcaacagacc tatttgcaaa ccactgcact catttccaat aaatcaatga cttatacatt
     2641 tattcgcatt tggcacaagc attgctagaa gtagtgctgg attttacgaa ggaggcaccg
     2701 tgcggtttca gcaaacgatc ggctcaccgg tttcatgttc aggcgttggt cttcattccg
     2761 gccagccggt cacactgaca cttcgcccgg cccctccaaa taccggcatc gtcttcatct
     2821 atcggcatga cccagaagaa acactcatcc ctgcatcgat ctcgaataag gtcccgacgg
     2881 aactctgcac cgccatcagc gtcaatggac accaggtcaa aacgatcgaa cacctcctcg
     2941 ccgccctggt gggaatggaa gtcgacaacg tctacgccga ggtgaatgca ggggaagtcc
     3001 ccgtcctcga tgggagctca agtccgtttg ttcggttgat tcgcgcggct ggcgtcatcc
     3061 cacagactcg gcggcaatcg tatgtcaaga ttatgcaacc catcgaagtg gtggacggat
     3121 cgaaacgggt gaggattgaa ccgtcgtcaa cgccgaaaat tacgtattcc atccattacg
     3181 atcacccctt gatccaaacg caaacctaca cctacacctg ctccgctcag gcgtttgagc
     3241 aagatatcgc gcaggcacgc acgttcggct tcctgcacga agtcgaagct ctctggtcca
     3301 gaggacttgg aaagggcggg accttggaca acaccatcgt gctgtccaaa gaaggcgtgg
     3361 tgaacgaatc aggcctgcgt ttctcgaatg aatttgtccg gcataaggtg ctggacctca
     3421 ttggtgacat tgcgctgctc ggcttcccgt tcatcggcca cgttgtggcc gaacgatcag
     3481 gccatgccat gcatacgaaa ctcgtcgaac agattctcct ccagcgcgac aaatgggcgc
     3541 tgatcaccgg ggaacatgcg gttgctgcat cagaatcgcg cccttccctc gggctgcttc
     3601 gccccgcgcc gtccctcgca atctaactgc ttctctcagc atggcttcac cacgcgtgcg
     3661 atccgccggc tccaggcggt gatcgaagca tctttcattt ttggggcata aaaaaacgcc
     3721 gggggcaagt ggccttaccc ccggcgtgta aatcctggca ggacttactt cttcttcgct
     3781 g
//
LOCUS       DAOQDS010000141         3728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_17894_length_3728_cov_15.496052, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000141 DAOQDS010000000
VERSION     DAOQDS010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17894_length_3728_cov_15.496052"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PK589_18490"
     CDS             complement(<1..220)
                     /locus_tag="PK589_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21656.1"
                     /translation="MDRLPIEEAIPPLRERLATGRSLLLTAQPGAGKTTRVPIALLDE
                     PWLSGQKLIMLEPRRLAARAAATYMAASL"
     gene            327..527
                     /locus_tag="PK589_18495"
     CDS             327..527
                     /locus_tag="PK589_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21657.1"
                     /translation="MQRLIWTSDKPKQTGWYWWRGLGEDMDPLILFVDQVGYFQWPDG
                     ASQEVGLTKGEWAGPIAPPEEQ"
     gene            530..1120
                     /locus_tag="PK589_18500"
     CDS             530..1120
                     /locus_tag="PK589_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21658.1"
                     /translation="MTMIALPASCERALLERFLRAEAMALWAVRAAQTQQLPRHVQTF
                     LQRHETDEQDHLRQFEGMLGTMSQRPATLPTVPSQWEMLAVLLFGYEALGLEFASLLA
                     AIRPDLSDILEDEEVHVGFFEKELKAILAGGETGAQQAREAARTWWKKLPRTVDRYLG
                     DASLAPFRTELRQHILSVIRQRFVALSLLPAEQTNR"
     gene            complement(1154..2470)
                     /locus_tag="PK589_18505"
     CDS             complement(1154..2470)
                     /locus_tag="PK589_18505"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ21659.1"
                     /translation="MSKSIVNVCIVSLLCTWGAWTGDAWGQSRLTGLPTGDGSSPVFS
                     TQMPGKEGPGASDPLWTDALWQADQQGVQRFFPQDGFVRVRGWIDGGYTYNTSNPSSN
                     FNGPYNAIDRDIPVLNQLYMIIEKPLTATGSNWGIGGRLDFMYGYDFFLTQSNGAERR
                     ENGAQRWNAQGQHYGLAMPQAYAEIGNQTFSVKVGHFYTIIGYEGVPSINNFFYSKSF
                     AYQFAGPFTHWGGLATWHVNDRVTLQGGVVNGWDSLDRTKDSATGLASITYAAPENLW
                     SLSLSMVTGNEPGAVATQFETRTRYSAIFTLHPAERWEYVLHHHYAYQNQGKFDGGTA
                     RWYGIDNYLYYAFADQWRAGLRFEWMRDEAGTRVGGNPVRDNPNLGPFAGSFYSVSAG
                     LNYRPHPNVLIRPEIRADWFDGQRSPYNDGLNKNQVLAAINGNLQF"
     gene            complement(2769..>3728)
                     /locus_tag="PK589_18510"
     CDS             complement(2769..>3728)
                     /locus_tag="PK589_18510"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNJ21660.1"
                     /translation="YLDFEKPIRELEEKIEKLASAESPKSGTQDEIRKLRTKLAQVEH
                     QLYTTLTPWQRTQLARHPQRPTSLDYISELSREFLELHGDRSFGDDRAIVGGFARFND
                     RSVMIIGHQKGKTLKERMQRNFGMPNPEGYRKALRLMRLAEKFNRPIITLIDTPGAYP
                     GIGAEERGQAEAIARNLFVMSRLTVPIISVVIGEGGSGGALALGVSDRILMLEHSVYS
                     VISPEGCAAILYDDPTKVPDAAASLRMTAQDLVGLGIVDEVIPEPLGGAHRDPRAMCD
                     RVAKALANQLYALVELPTDQLIAERDQKFRKIGVVGGLVPVGA"
BASE COUNT          703 a         1045 c         1220 g          760 t
ORIGIN      
        1 cgaggctcgc ggccatgtag gtggcggcgg cgcgcgcggc cagccggcgt ggttccagca
       61 tgatcagctt ctgtccgctc agccagggtt cgtcgagcag ggcgatggga acgcgggtgg
      121 tctttccagc gcccggctgt gcagtcagga gcaacgatcg gccggtggcc agccgttcgc
      181 ggagcggggg aatcgcctct tctattggta atcggtccat ggtagggtgc gcgctgcaat
      241 gacgcgtcaa cgggactgta gcagaaacgg gggctgcgcg ccgagtgcca atcagaggtc
      301 gcgcgagatc aggagtgtaa cggacgatgc aacgattgat atggacgagt gacaaaccga
      361 agcagacggg ttggtattgg tggcgtgggc tgggagagga tatggatccg ctcattctgt
      421 ttgtcgacca ggtgggctat tttcagtggc cggatggggc gtcgcaggaa gtcgggttga
      481 ccaagggcga atgggccggt ccgatcgcgc cgccggaaga gcagtgagtg tgacgatgat
      541 cgcgctgccg gcctcctgcg aacgagcctt gctcgaacga ttcctgcggg ccgaagccat
      601 ggccttgtgg gcggtgcgcg ccgctcagac gcaacagctt ccccgccacg tgcagacctt
      661 tctccagcgg catgagacgg acgaacagga tcatcttcgg caatttgaag gcatgctcgg
      721 gacgatgtct caacggccgg ccacattgcc gacggtgccg tcgcagtggg aaatgctggc
      781 agtgctgttg ttcgggtacg aagcgctggg gctggaattc gcctcgttgc tggccgcgat
      841 caggccggat ctttccgaca tcctggaaga cgaagaggtg cacgtgggat tcttcgagaa
      901 ggaattgaag gcgattctgg ccggtggaga gacgggtgca cagcaagcgc gagaggccgc
      961 gcgtacctgg tggaagaagt tgcctcgcac ggtggatcgc tatctcggcg atgcgtcgct
     1021 ggcgcctttt cgaaccgaac tccggcaaca tatattgtcg gtcatccggc aacgcttcgt
     1081 cgcgttgagc ctgctgcctg cggagcagac aaaccggtaa cactccggcc tgcctgctcc
     1141 atgccatccc actctagaac tgtaggttcc cattgatcgc cgccaatacc tgatttttgt
     1201 tcaggccgtc gttgtatggc gagcgctgcc cgtcgaacca atcggctcga atctccgggc
     1261 gaatcagcac attggggtgc ggacggtagt tgagtccggc gctgaccgaa taaaaagagc
     1321 cggcaaacgg cccgagattc gggttgtcac gcaccggatt tcccccgacc cgcgtaccgg
     1381 cctcgtcccg catccactcg aagcggaggc ccgctcgcca ctgatcggca aaggcgtagt
     1441 acaggtaatt gtcgatgccg taccagcgtg cggtgcctcc gtcgaacttg ccctggttct
     1501 gatacgcata gtgatgatgc aacacatatt cccaccgttc agccgggtgc agggtgaaaa
     1561 tggcgctata gcgggtgcgg gtttcaaatt gtgtggcgac cgcgccgggt tcgtttccgg
     1621 tgaccatcga caaggaaagt gaccacaaat tctcgggcgc ggcgtatgtg atgctggcca
     1681 gacctgtggc actgtccttt gtccgatcca gagaatccca gccgttcacc acgccgcctt
     1741 gcagcgtcac ccggtcgttc acatgccagg tggccaagcc gccccagtgg gtgaaggggc
     1801 ccgcgaattg atacgcgaaa gacttggagt agaagaagtt attgatggac ggcacccctt
     1861 catagccgat gatcgtgtag aagtggccga ccttcactga aaacgtctgg ttgccgattt
     1921 ctgcgtaggc ctggggcatt gccagtccgt aatgctggcc ctgcgcgttc caacgctgcg
     1981 cgccgttctc ccgtcgctct gcgccgttgc tctgtgtcag gaagaaatcg tagccgtaca
     2041 tgaaatccaa gcgcccgcca atgccccaat tgctccccgt ggccgtcaac ggtttctcga
     2101 tgatcatgta caactggttc aagacgggaa tgtcccggtc aatggcgttg tagggtccgt
     2161 tgaagttgct gctggggttg ctggtattgt acgtataccc gccgtcgatc cagccgcgta
     2221 cgcggacgaa tccgtcctgc ggaaaaaacc gttggactcc ctgctgatcg gcttgccaca
     2281 aggcatcggt ccagagcggg tctgatgcgc cgggcccttc ctttccaggc atctgcgtgg
     2341 agaagaccgg ggaagaccca tcgcccgtcg ggaggccggt gagccgtgac tgtccccatg
     2401 cgtctccggt ccacgcgccc caggtgcata acaacgacac gatgcatacg ttcacgatcg
     2461 acttcgacat acctgtccac acctcctgtg aaaatctccc atggcctgtt cagtgatgac
     2521 cctgcgcggg cgggcggcag tgtaccaact cgccgggcga tgggaaatca ggctaaggct
     2581 gtaggtggtt tcagggaggc ttgataccgg atccggatgc ggagcatgac gaacgcctga
     2641 gcaggctgag tatggtgatg ccgagaggcg acaccaaata atcgtcggat agcgacgggg
     2701 aagcgagcga gttcttgcgg cttgtcagaa tcgcgtagga acgaacgggg cggggaattc
     2761 gagggtgact aggctcctac ggggacaagt ccgccaacga cgccgatctt gcggaacttc
     2821 tggtcgcgtt cggcgatgag ttgatcggtc ggcaattcaa ccagcgcgta cagctgattg
     2881 gccagcgcct tggcgacgcg atcgcacatg gcccgcgggt cgcggtgggc accgccgagt
     2941 ggctctggaa tcacttcatc cacgatgccg agtcccacga ggtcctgcgc ggtcatgcgt
     3001 agggacgctg cggcgtcggg caccttggtc ggatcgtcat acagaatcgc cgcgcagcct
     3061 tcgggggaaa tgacggaata gacggaatgt tccagcatca aaatacgatc gctcacgccg
     3121 agcgcgagcg cgccgccgct gccgccttcg ccgatgacaa cggaaataat gggcacggtc
     3181 agtcgcgaca tgacgaagag gtttctggcg atcgcttccg cctgtccgcg ctcttccgct
     3241 ccgatgccgg ggtaggcgcc cggtgtatcg atcagcgtga taatggggcg gttgaacttc
     3301 tccgccaggc gcatgagccg cagcgctttg cggtagcctt ccggattggg catgccgaag
     3361 ttgcgctgca tgcgttcctt gagggttttc cccttttgat ggccgatgat catgaccgag
     3421 cgatcgttga agcgggcgaa tccgcccaca atcgcgcgat cgtcgccgaa gctgcgatca
     3481 ccatggagtt ccagaaattc gcgggagagt tcgctgatat agtcgaggga cgtgggacgc
     3541 tgggggtgac gggccaattg ggtccgttgc cagggcgtca gggtggtgta gagctgatgc
     3601 tcgacctgtg cgagtttggt tctgagcttt cggatctcgt cctgggttcc ggatttgggt
     3661 gactcggcgg aagccagctt ctcgattttt tcttcgagtt cgcggatcgg tttttcaaag
     3721 tcgaggta
//
LOCUS       DAOQDS010000142         3665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_18453_length_3665_cov_15.046537, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000142 DAOQDS010000000
VERSION     DAOQDS010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18453_length_3665_cov_15.046537"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..492
                     /locus_tag="PK589_18515"
     CDS             157..492
                     /locus_tag="PK589_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21661.1"
                     /translation="MLCSFDSDLRAEMARQDRAEAREQERDKLVEIEATRLLEGDFDP
                     LAPANFYEAVAETADTTSVGRMLNALREGRHEHAGQILEALTVAYWQRQAQRRAENIV
                     ARRMAEVEE"
     gene            489..629
                     /locus_tag="PK589_18520"
     CDS             489..629
                     /locus_tag="PK589_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21662.1"
                     /translation="MKFILIVILAGHAQYAVGPFSSHVECTKYPIAAEAERVCIPLTR
                     GK"
     gene            652..978
                     /locus_tag="PK589_18525"
     CDS             652..978
                     /locus_tag="PK589_18525"
                     /inference="COORDINATES: protein motif:HMM:NF022224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2591 family protein"
                     /protein_id="HNJ21663.1"
                     /translation="MKIKTSELIGPALDYMVHVANGNSMASCSYWLEKYEDEQRRALC
                     EDYSTNWSQGGPIIEREDLSVGREFTAFGKGGVRGEGPTPLIAAMRCYVASKLGDEVD
                     IPEELT"
     gene            975..1154
                     /locus_tag="PK589_18530"
     CDS             975..1154
                     /locus_tag="PK589_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21664.1"
                     /translation="MIITNFLDSDRQELNGEAVELIRTVTEPEPEFDEEVLPMMYVKV
                     LRTGEVVLCFPEELK"
     gene            1151..2050
                     /locus_tag="PK589_18535"
     CDS             1151..2050
                     /locus_tag="PK589_18535"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNJ21665.1"
                     /translation="MKPLVIYHANCTDGFGAAFSAWLALGDEAEYVPMNYNSINPADL
                     ADLTDDREVYILDFSLPRVQMESVFRLAKRVVWLDHHKTAFEMWRGKYKKGMIHQSGE
                     FDKQLILLNDNKSGALLAWEYFHPNTEVPMLIQHIDDRDRWQWKLRCSAEIHAALGSM
                     QPWSFEQWEELIQPEHYGSLCTEGAAILRAQDQTVQQIVGHARKSYIEHPHGGDNDWV
                     FVTGLAVNSPVHQSEVGHELANQSGTFGLAWYLAEDNTVRCSFRSNGDYDVSAIARVF
                     GGGGHRNAAGCEVSIETLLDWLK"
     gene            2047..2340
                     /locus_tag="PK589_18540"
     CDS             2047..2340
                     /locus_tag="PK589_18540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21666.1"
                     /translation="MKKTETAKRIKELERQSMEHLAGQTHRYHFADAGVAKITMDRFK
                     ASGVILTITALGGEELVGPVLIRGGLSDATVSAIRVDLVRSYQESIELKPKGV"
     gene            2337..2477
                     /locus_tag="PK589_18545"
     CDS             2337..2477
                     /locus_tag="PK589_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21667.1"
                     /translation="MKRPTQKSVAIQVKTGGRWYEFGLMNYVERRELADILRKTAEEL
                     EK"
     gene            2577..2834
                     /locus_tag="PK589_18550"
     CDS             2577..2834
                     /locus_tag="PK589_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21668.1"
                     /translation="MTPGTLNPGEIHPAAQDAMAYIRSVPTNGLFNLVEVFASVALSG
                     NRNAEICGETLQRILNGEPVSDRYILGLAWSMKTMETMEVE"
     gene            2838..3062
                     /locus_tag="PK589_18555"
     CDS             2838..3062
                     /locus_tag="PK589_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21669.1"
                     /translation="MNKFKISPVFAAKLLNGEANLVGGKLLLDSIKISIVGGKVRLTL
                     MTKGIDVMHLDAEVYVDRGDTVTVTGLTIK"
     gene            3271..>3665
                     /locus_tag="PK589_18560"
     CDS             3271..>3665
                     /locus_tag="PK589_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21670.1"
                     /translation="MDNPSMDGLDMDLAHAAEEIQRWQRAAEANIGENVRLRHEKDEG
                     WKEAAIAWEVCASIHEKWAKGKDALYSTRHADFVKHAADARAKLTPDATALHAWFKEQ
                     LGEPVMEFVQSPNGNYIEMKWQSVYEAKLG"
BASE COUNT          889 a          991 c         1068 g          717 t
ORIGIN      
        1 gcgactcgcc gactggtcga tttatgcgcg agtgatcgcc aagacaaagc cccacaacct
       61 ggtcagcggg tcgtgggttg agccggcggc agaagcccct caccaaagcc aaaacggaga
      121 ccgctaatgc aacacacttc cctaccggag ataaccatgc tctgttcatt tgacagtgac
      181 ctgcgcgccg agatggcccg ccaggatcgt gccgaggctc gcgaacaaga gcgcgacaag
      241 ctcgtcgaga tcgaggccac ccgcctgctt gaaggcgatt tcgatcctct ggcgccggcc
      301 aatttctacg aggctgtggc cgagacggcc gacaccacga gcgtcggcag gatgctcaac
      361 gctctgcgcg aagggcgcca cgagcacgcc ggccaaatcc ttgaggcgct gaccgtcgcc
      421 tactggcagc gacaggccca acgaagggcc gagaacatcg ttgctcgacg catggcggag
      481 gttgaagaat gaagttcatt ttgatcgtca tcctggcggg ccatgcccag tacgccgttg
      541 gcccgttcag cagtcacgtg gaatgcacca agtaccctat cgccgccgag gcggaacggg
      601 tctgcattcc gctcaccagg ggaaaatgat gcgttatgag gaggtcgaag aatgaagatc
      661 aaaactagcg aactcatcgg cccagcgctg gactacatgg tgcatgtagc gaatggcaat
      721 agcatggcgt cgtgttccta ctggttggaa aagtacgaag acgaacaacg tcgcgccctt
      781 tgtgaagact attcaaccaa ctggtcgcaa ggcgggccga tcattgagcg agaagacctg
      841 tccgtgggtc gagaattcac tgcgttcggt aaaggcggtg ttcgcgggga aggccccacc
      901 ccccttatcg ccgccatgcg ctgctacgtc gcctcgaagc ttggcgatga ggtggatatt
      961 ccggaggaac tgacatgatt atcaccaatt ttcttgactc tgatcgccaa gaactaaacg
     1021 gcgaagcggt cgaattgatt cgtactgtta ctgagcctga gcctgagttc gacgaagaag
     1081 tgctgccgat gatgtacgtt aaagtgctac gtacaggtga ggtagtcctg tgtttccccg
     1141 aggagctgaa atgaaaccac tcgtgattta ccacgccaac tgcaccgacg ggttcggtgc
     1201 cgcgttctct gcgtggctgg ccctcggtga cgaggctgag tacgtgccga tgaattacaa
     1261 cagcatcaat ccggcagacc ttgccgattt gaccgacgac cgtgaagttt atatcctcga
     1321 cttctccctg ccccgcgtgc agatggaaag cgtattccgt cttgccaagc gtgtcgtgtg
     1381 gctcgaccac cacaagacgg cgtttgaaat gtggcgtggg aagtacaaaa agggaatgat
     1441 tcatcagtcg ggagaattcg acaagcaatt aatcctgctc aacgacaaca agtccggtgc
     1501 cctccttgcc tgggaatact tccaccccaa caccgaagtc ccgatgctga tccagcacat
     1561 cgatgaccgt gaccgctggc agtggaagtt acgctgcagt gcagagattc acgccgcact
     1621 gggttcgatg caaccgtgga gcttcgagca gtgggaagaa ctcattcagc ctgaacatta
     1681 cggttcgctc tgcaccgaag gagccgccat cctccgcgcc caagatcaga ccgtacagca
     1741 gatcgtcggc catgcgagga agtcttacat cgaacatcca cacggaggag acaacgattg
     1801 ggtattcgtt accggtctcg ccgtcaattc tccggttcat caatctgagg tcggacatga
     1861 actcgccaac cagtctggca cgttcggact ggcatggtac ctggccgagg acaacactgt
     1921 ccggtgctcc ttccggtcca atggggatta cgatgtgagt gctatcgcca gggtattcgg
     1981 cgggggcgga catcgaaacg ctgctggctg cgaggtcagc atcgagactc tgctggactg
     2041 gctgaaatga aaaagacaga aacggcaaag cgtatcaaag aactcgaacg ccaatcgatg
     2101 gagcacttgg ccgggcagac gcatcgttac catttcgccg acgctggggt ggcgaaaatt
     2161 acaatggacc gcttcaaggc ctccggtgtg attctgacta ttacagccct cggtggtgaa
     2221 gagctggttg gacctgtgct catacgcgga ggactctcgg acgcgaccgt ctctgcaata
     2281 agggtagacc ttgttcgcag ttaccaagaa tctatcgaac tgaagccgaa gggagtttga
     2341 aacgaccgac ccagaagtcc gtcgccatcc aggtaaaaac cggcggccgc tggtatgagt
     2401 tcggcctcat gaattacgtc gaacgcaggg aactcgcgga cattctccgc aaaacggcag
     2461 aggagcttga gaaatgacac ctgaaatcat tgaagcaatt gggaagttcg ttgtagtgcc
     2521 agtttgttgc gctgtaatcg tctggatcgc ttgcagggat tagaaacgga gacgacatga
     2581 cccccggaac cctcaaccca ggcgaaatcc accccgccgc tcaggacgcc atggcctaca
     2641 tccgtagcgt tccaactaac ggtctgttca atctagtcga agtcttcgct tccgttgcgc
     2701 tgtctggaaa tcgaaacgct gagatttgcg gggaaactct gcaacgcatc ctcaacggtg
     2761 agccagtgtc ggacagatac atcctcgggc tcgcttggtc catgaagacg atggagacga
     2821 tggaggtgga atgaagaatg aataagttca aaatcagccc tgtctttgcc gcaaaactcc
     2881 ttaacggcga agcgaatctg gtcggcggga aactgcttct ggacagtatt aagatcagca
     2941 tcgtcggcgg caaagtccgt ctgacactga tgaccaaagg catcgatgtc atgcaccttg
     3001 acgcagaggt ttacgtagat cgcggcgata cggtcactgt gacaggatta accattaaat
     3061 gatctattcc ggggcgctac cggagccaca ggaagagatt tacaagtgca cttcatgcgt
     3121 ggagaggcac ggatcgttcg agccgcagcc cggaataaag ccggagcact cctgcgggca
     3181 cctgacgcat aacgcagagc tgtgcggcgg gccgtcaggc ccgtccgggt tgaatgattt
     3241 gttgggcgtg atgcgcccga ggagactgaa atggacaacc caagcatgga cggccttgac
     3301 atggacttgg cccatgccgc cgaagagatt caacggtggc agcgcgctgc cgaagccaac
     3361 attggggaga acgtgcgatt gcgccacgaa aaagatgaag ggtggaaaga ggcggcaata
     3421 gcctgggaag tctgcgcttc gatccacgag aagtgggcca agggcaaaga cgcactgtat
     3481 tcgactcgcc atgctgattt tgtgaagcat gcggctgacg cacgggcgaa actgacgccc
     3541 gacgccaccg ccctgcacgc atggttcaag gagcaactcg gggagcctgt tatggagttt
     3601 gtacagtccc ccaacgggaa ttacatcgag atgaagtggc aatcagtata cgaggccaaa
     3661 ctcgg
//
LOCUS       DAOQDS010000143         3617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_18837_length_3617_cov_14.163111, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000143 DAOQDS010000000
VERSION     DAOQDS010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18837_length_3617_cov_14.163111"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..834
                     /locus_tag="PK589_18565"
     CDS             97..834
                     /locus_tag="PK589_18565"
                     /inference="COORDINATES: protein motif:HMM:NF033587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HNJ21671.1"
                     /translation="MQTSKPLYHRHRFPDAVISRAVRWYFRFQLSLRDIEELLFERGV
                     VVSYETIRRWCDKFGAHFTHRVKQARPAPGATWHLDEVFVNVGGEPYLLWRAIDQHGA
                     ELDILLQKRRDKAAAKRFFQRLLAQYPNGPRKIVTDQLRSYPAAKVEIPELADVKHVF
                     VKAAARVNNRAENSHQPTRERERRMKGFRSPGRTQTFLSSFGPIRQHFAIKRHQLGAV
                     RYRNQLARRFAIWHLVTGVTQNPSTAV"
     gene            complement(888..1151)
                     /locus_tag="PK589_18570"
                     /pseudo
     CDS             complement(888..1151)
                     /locus_tag="PK589_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            1288..2541
                     /locus_tag="PK589_18575"
     CDS             1288..2541
                     /locus_tag="PK589_18575"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNJ21672.1"
                     /translation="MDHVENAMDHVSPWATAPKLPSTHYVDNRIYTDPVIFEEEREKI
                     LAATWRLVCHESELPEPGDYRAKEIAGVPVVILRSEDRSIRAFFNVCPHRGARLVRDE
                     RGNAKKGLQCFYHLWTFALDGKCTSITRSKGYDGCSLKKDDVGLRSVRTEIFCGMVFV
                     CLKDDVPPLEQFLGGMADHMKTHLGQEELEVFHYHRAIINTNWKLFVDNNSELYHEFL
                     HVLNRRTAVAHKSYNERNWLLYQNSHNIIQQGVIHYDSYGLGERSEGALPGMQPNGMV
                     VMLLFPDVMLNIRATVMRIDTMTPLAPGKTLVEWRGVGLKSDTPDVRAMRIQHHNEVW
                     GPAGRNLPEDIAAVETQWETMVQGGSRYSLFAREEGLKPQDDANLRAFYQEWGRCLGR
                     APNDPFRHGNVDTDVSHSIKEITNV"
     gene            2534..3007
                     /locus_tag="PK589_18580"
     CDS             2534..3007
                     /locus_tag="PK589_18580"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNJ21673.1"
                     /translation="MSDVASQLFEREAIRELIARVGRLLDTKDFAAVIASFAEPGSYV
                     LRAYSPEIGKEMIWMSVKRAELLRLLKESAEHVHDLADRTHQITVDQISLEKGEALAR
                     STFSVFRTDMKGQSDLFAVGHYEDRLVKNGDTWLIDSRTVRLHTRMFTVPTPLPL"
     gene            3019..3348
                     /locus_tag="PK589_18585"
     CDS             3019..3348
                     /locus_tag="PK589_18585"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNJ21674.1"
                     /translation="MNTQPIKLCARTDIPEGEMIEMSVPGTDNSLLLVSTKGAIRAFQ
                     NKCPHMDIPLCQGAFDGEVITCTEHLWQFDAQTGAGIEPEDAKLQQYPVSVIGDEVFV
                     ELPEAAE"
BASE COUNT          846 a         1021 c         1007 g          743 t
ORIGIN      
        1 ggagacaagt cggcgcgcgt agcggcgcaa gatgagatcg gggatgtctg ccaggggcat
       61 tgtcacggta acgattgaag ctggtaaagt cgatcgatgc agacatccaa gcccctttac
      121 caccgccatc gttttccaga cgccgtcatc agtcgcgcgg tgcgctggta tttccgattt
      181 cagttgagct tgagggatat cgaagagctg ctcttcgagc gcggcgtggt cgtcagctac
      241 gaaacgatcc ggcggtggtg tgacaagttc ggcgcgcact ttacccatcg ggtaaagcag
      301 gccaggcctg ctcccggcgc aacgtggcat ctcgacgagg tcttcgtcaa tgtcggcggt
      361 gagccttatc tgctgtggcg cgccatcgac cagcatggtg ctgagctcga tatcctgctg
      421 cagaaacgcc gcgacaaggc ggcagccaag cgcttcttcc agcgtctgct ggcccaatat
      481 ccaaatggcc cgcgtaagat cgtcaccgac cagctacgca gctatccggc ggcgaaagtc
      541 gagattcccg aactggctga cgtcaagcat gtgttcgtca aagctgcagc ccgggtcaac
      601 aaccgggccg agaatagtca tcagccaaca cgcgaacgcg aacggcgcat gaaaggcttt
      661 cgctcgccag gacgaacgca aacattcctg tccagcttcg gaccgatccg acagcatttc
      721 gccatcaagc gccaccaact cggcgctgtg cgctatcgta atcaactcgc cagacgcttc
      781 gctatctggc atctcgtcac tggcgttacc caaaatccgt ctaccgcagt ctgagcacca
      841 tttcaccagc tctcacttcg ccaatacggc gttaagttga caaggccctt cacatatgtc
      901 tcgtcgacgc gccaagcgga gccggctgca gtggcatacc ggttccagcg tttctcgaac
      961 tcgggaacga agcgctgcac ccaacgcagg atggttgtat gcgcgacagt caaaccgcgc
     1021 tcggccatca tttccaccag atcgcgcgat gaaagcttga agcgcaagta ccagcgcaca
     1081 cacagaatga tgatctcccg ctcaaagtgt ctgcccttga acagctcttc gatggtcttg
     1141 cgcttggcca tgtcagtcaa atcgaccgat tctaaccgat gccgctattt gcaccacaac
     1201 cgtcgtgtgg tggttgacag cttgaggtac acatgccatc atttattata aataaatgat
     1261 gtttattata cctaaacgga gaacgcaatg gatcatgtgg agaacgcaat ggatcatgtc
     1321 agtccctggg ctactgcccc taagcttccg tctacgcact atgtagacaa ccgaatctat
     1381 acggatccgg tgattttcga ggaagagcga gagaaaatcc tggctgcaac gtggcggctt
     1441 gtctgtcacg aatcggaact gccagaaccc ggcgactacc gggcgaagga aatcgccggg
     1501 gtaccggttg tcatcctgcg cagcgaggac cggtcgatta gggcgttctt taacgtttgc
     1561 ccacaccggg gcgcgcgcct agtgcgcgac gagcgtggaa atgcgaagaa gggcctgcag
     1621 tgcttttatc atctatggac gtttgcgctc gacggtaagt gcacaagcat cacccgaagc
     1681 aagggatatg acggctgcag tctgaaaaaa gacgacgttg gactgcgctc tgtccgaacg
     1741 gaaatcttct gcggcatggt gttcgtttgc ctgaaggacg acgtgccacc actcgagcaa
     1801 ttcttgggtg gtatggcgga ccatatgaag acccaccttg gccaggaaga gcttgaggta
     1861 ttccattacc atcgcgcgat catcaatacg aactggaagc tcttcgtaga caacaactcc
     1921 gagctctatc atgaattcct gcatgtgctg aaccggcgca ccgccgtcgc acacaagagc
     1981 tataacgaac gcaattggtt gctttaccag aattcccaca acatcattca gcagggtgta
     2041 attcactacg acagttacgg gctgggggag cggagcgagg gcgcattgcc gggaatgcag
     2101 ccgaacggca tggtcgtcat gctgctgttt cctgacgtga tgctcaatat tcgcgcgacc
     2161 gtgatgcgta tagacacaat gacgccactc gcacccggca agaccctcgt cgaatggcgc
     2221 ggcgtagggc tcaagtccga tacaccggac gtccgcgcaa tgcggatcca gcaccacaat
     2281 gaggtatggg gaccggccgg tcgcaatctc ccagaggata tcgccgccgt cgaaacgcag
     2341 tgggagacca tggtccaggg tggatcgcga tacagcctct ttgccaggga agaagggctg
     2401 aaaccgcagg acgatgccaa cctccgcgcc ttctatcagg aatggggtcg gtgccttgga
     2461 cgcgcgccga atgatccgtt caggcacggt aatgtggata ccgatgtttc tcattcaata
     2521 aaggagatca ccaatgtctg acgtcgcaag ccaactcttc gagcgtgaag cgatacgcga
     2581 gctgattgcg cgcgtggggc ggctgcttga cactaaggat tttgctgccg tcatcgcttc
     2641 ctttgcagaa ccggggagct atgtgttgcg cgcatacagt cccgagattg gcaaggaaat
     2701 gatttggatg tcggttaaac gcgccgagct tctacgcctg ttgaaggagt cggcggaaca
     2761 tgtgcatgac ctggccgacc ggacgcatca gatcactgtg gatcaaatca gcctcgaaaa
     2821 aggcgaggca ttggcgcggt cgaccttttc agtgttccgt actgacatga agggacagag
     2881 cgacctgttc gccgtgggcc actatgagga tcggctcgtg aaaaacggag acacctggct
     2941 catcgacagc cggacagtac ggctccatac gcgcatgttc acagtgccaa cccccttgcc
     3001 gctttaacga gataactgat gaatacgcaa ccgatcaagc tatgtgccag gaccgacatt
     3061 ccggagggtg agatgatcga aatgtccgtc ccgggcactg acaacagcct gctcttggtc
     3121 tctaccaaag gagcaatccg tgccttccag aacaagtgtc cacatatgga catcccgctt
     3181 tgccaaggcg cctttgatgg tgaagtaatc acttgtacag aacatctttg gcagttcgac
     3241 gcacagacgg gcgccgggat tgaacccgag gacgcaaaac tgcaacagta tcctgtgagc
     3301 gtcatcggcg acgaggtgtt cgtcgaactc ccggaagcgg ccgagtaatt gcctggtctt
     3361 gccggataga accagcaaga ccaggcaata aaggcggata tcgcccattt ctttccgcac
     3421 tagtggaatc ctcatcacca actgccggac tctcgactaa tgaccatgcc tcatcgaatc
     3481 cattttgacg ccgccaacgg cttcgacgct gcaacggacg aaccgctcct gatttcaggc
     3541 ctgcgggcag gctatgcgct tccgtatgaa tgcgcgacag gaacttgtgg ctcatgccgt
     3601 acccagatca ttgaagg
//
LOCUS       DAOQDS010000144         3547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_19484_length_3547_cov_22.175258, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000144 DAOQDS010000000
VERSION     DAOQDS010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19484_length_3547_cov_22.175258"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PK589_18590"
     CDS             <1..187
                     /locus_tag="PK589_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21675.1"
                     /translation="TLTAETLHSYGFPQAPYPSPTYRHFKNHCYFYKMMTFDRRLVAP
                     VSSGIIVNQRASHDVCG"
     gene            287..769
                     /locus_tag="PK589_18595"
     CDS             287..769
                     /locus_tag="PK589_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21676.1"
                     /translation="MTNLTSTLRLQHLFISNLLEAAQWIGVEKVEAQRKLRAAKIHLL
                     AHFKLEDTLLYPVLFEAAEKNTELKYTLQFLEKDMAGVSKVMMSFFEQFHISGTHHHT
                     TQDIGNILFLLQTRIRREEDTLFAEFDKLKAAARTIVAVQIPDSRSSILASSHSIHPM
                     "
     gene            880..1173
                     /locus_tag="PK589_18600"
     CDS             880..1173
                     /locus_tag="PK589_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21677.1"
                     /translation="MPDLTKNEAIIAALESAGYQEIRLTVVPKRFLFSDGRCHTELEF
                     SRNYFDDTTLIALQGQMGEHILPAIKQQPGKKVYVDARGISIIPSTHGRTDKH"
     gene            1370..1807
                     /locus_tag="PK589_18605"
     CDS             1370..1807
                     /locus_tag="PK589_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21678.1"
                     /translation="MLPEMLLFATIHGTQALPVSDKVPYESAEASAKLFSEDEIRVVK
                     GIILDTTAVRLKDGVEFMQMEVETERGPVRVHLGPTWYMNEYHERFDVNKGRLVTAVC
                     SPALVDGRHVLVAAELANADGQQRMRLRHPDGTPAWVGGEHVK"
     gene            1851..2021
                     /locus_tag="PK589_18610"
     CDS             1851..2021
                     /locus_tag="PK589_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21679.1"
                     /translation="MDEVRGLQFTARRLATQLDLLIQALEDAKPSQEIQELCREGRVV
                     LDYATTVLGGDE"
     gene            complement(2298..2789)
                     /locus_tag="PK589_18615"
     CDS             complement(2298..2789)
                     /locus_tag="PK589_18615"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNJ21680.1"
                     /translation="MKRVNVARRMKPKETTCVSPCISTLPRHRLVELLWSFAPAYQRW
                     SESLLVEKGLSPQRLRILGSIHERGPRIMSELKKELGVTATNVTALVDSLENDGLVVR
                     RRHPTDRRATVIELSDKVMRELSPRCTEYKEQVAELFSDLSDQECKAFVKTLEKLWSR
                     LQG"
     gene            2970..>3547
                     /locus_tag="PK589_18620"
     CDS             2970..>3547
                     /locus_tag="PK589_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ21681.1"
                     /translation="MRTIRYTSWLLAVLLTAAGCNNEQASSGGQAPPPPEVGVAQVIS
                     KPVQQWDEYTGRISAIDTVELRPRASGYVQRVAYKEGQDVKQGDLMFLIDPRPYRAAL
                     ENAQAQLARARVAQQLETIRNKRAQALIDDNAISHEELDLRRAAQAQSAADVHAAEAA
                     VATAKLNLSFTEVRAPVSGRASRALVTVGNLAT"
BASE COUNT          820 a         1055 c          955 g          717 t
ORIGIN      
        1 aacgctgaca gctgaaaccc tacactccta tggatttccc caggctccct acccgagtcc
       61 aacctacaga catttcaaaa atcactgcta cttttacaag atgatgacgt tcgaccgcag
      121 gctcgtcgcg ccggtatcct ccggcatcat cgtgaaccag cgggcgagcc atgacgtctg
      181 tggatgatgg tcaggaccgc gacgtctgca tcaccgattg gtgacgactt ccgactgggt
      241 cctgtgtcag ctgagccgta gaccgaatga aggagcaaga agtctcatga caaacttaac
      301 ctcgacactg agactgcaac atttgttcat ctcgaactta ttggaagccg cgcagtggat
      361 cggcgtggaa aaagtcgaag cgcaacggaa actgagagcg gcgaaaatac accttttggc
      421 gcacttcaaa ttagaagaca cgctcctgta tcccgtctta ttcgaagccg ccgaaaagaa
      481 tacggagttg aagtacacgc tgcaattcct tgagaaagac atggcgggcg tctcgaaagt
      541 gatgatgagc ttttttgagc agttccacat cagtggcaca caccatcaca cgacacagga
      601 cattggcaac atcctctttc tgttgcaaac acgcatacgt cgagaggaag acacgctgtt
      661 cgcagaattc gacaaactga aggctgcggc ccgcaccatc gttgctgtac agatccctga
      721 ttcccgatct tccattttgg cttcctctca ctcaatccat cccatgtgaa cgacggcctc
      781 cacgatgcag tccaagcccg cgctcacatg ctcgacgacc gacgcatcag cagatcaacg
      841 cgtgccggaa tcagagatag acggagagag ggaggcacga tgccagacct gacgaagaac
      901 gaggccatca ttgcggcact ggaatcagcc ggctatcagg agatcaggct gacagtcgtc
      961 cccaaacgct ttctgtttag cgatgggcgc tgtcacacag agctggaatt ctccaggaac
     1021 tattttgacg ataccacgct catcgcgctg caaggccaga tgggagagca catcctcccg
     1081 gccatcaagc agcagccggg aaagaaggtg tatgtggacg cccgcggaat tagtatcatt
     1141 ccttccacgc acggccggac ggacaaacac tagtagctcc tcccatcctt tccgctccgc
     1201 agcggatccc cccactccag ctaacctgtg atgccggcag gatccagccg agcctctctc
     1261 cccttttcct atttcgatgg tgcagggcag catgcgcggg acactcctag tttgctgcag
     1321 gccgactggg tgctacggtc aactcataca ttcacaaagg agactcggca tgctgccaga
     1381 aatgctcctc ttcgccacga ttcatgggac acaggctctg cccgtatccg acaaggtacc
     1441 ctacgaatca gcagaagcct cggcaaaact cttctcagag gatgagattc gagtcgtcaa
     1501 aggcatcata ctggatacca ccgctgtcag attgaaagat ggggtggagt ttatgcagat
     1561 ggaagtagaa accgaacgtg gtccagtccg ggtgcatctg ggaccgactt ggtacatgaa
     1621 tgaatatcac gaacgttttg acgtgaataa aggtcgactc gtgacggccg tctgtagtcc
     1681 ggctctggta gacggcagac acgtattagt ggccgccgag ctggcgaatg cagacggtca
     1741 gcaacgcatg cgtcttcggc acccagacgg cacgccggct tgggtcggcg gcgaacacgt
     1801 caagtgacgg atgatcggtc gcagtcagca gtctcgcctc aaggagcccc atggatgaag
     1861 tacgtggact ccaattcact gcgcgtcgtc ttgccacaca gttggacctg ctgattcagg
     1921 ccctcgaaga cgcgaagcct tcccaggaga ttcaggagtt gtgccgagag ggtcgggtgg
     1981 tactggatta tgccactacc gttctgggag gagatgaatg agactcagag ggacattcca
     2041 cggcttggaa gcatgacgtc tggatcgtac gtgtttgaca ctctcgtcgt ggagggatat
     2101 cgtgatcgta tgttcgctcg tctcttctca atactgtcgc tcgccaccct cgcactcctg
     2161 ttcaagcgac ttgtgacgcg tcgaccgccg tctcgcccgt accgcaaggc tgaatccaag
     2221 tggcctagtc gaagctaccg cgaaccttcc tgaattctgc tgcggtacct gcaaagactc
     2281 tttcttacga ctgtgcttta cccctgcaat cgactccaca gcttctcgag cgttttcacg
     2341 aatgctttac actcctggtc gctcaggtct gaaaacagtt cggcgacttg ctctttatat
     2401 tccgtacagc ggggcgagag ctctctcata actttgtccg agagctcgat caccgtggcc
     2461 cggcgatccg tgggatgtcg tctccgcacc accaatccat cgttttccag cgaatcgacg
     2521 agcgccgtga cgttcgtcgc cgtcacgccg agctcctttt tcagctcgct catgatcctg
     2581 gggccgcgct catgaatgga gccgaggatg cgcaaccgct gaggcgataa ccccttctcc
     2641 accagcaacg actccgacca ccgctgatag gcaggagcaa agctccacaa aagctcaacc
     2701 aaccggtgcc ggggcaacgt cgagatacag ggagagacgc atgtcgtctc cttgggcttc
     2761 attcgcctgg caacgttgac acgtttcatc gtacagatac ggtacttcaa gaacgcgaca
     2821 cctttcaatc ataaattatt catagctgca tcattcatat attgactatg tttcacaccg
     2881 tgccggatac tgcacgacag gggtgaggaa gaaccccgga ctcttgcact gacgtcatcg
     2941 ttcaccatcg atcatccaac aggtacacca tgcgcactat ccgatataca tcctggctgc
     3001 tggcagtgct tctgaccgca gccggctgca acaacgaaca ggccagcagc ggcggccagg
     3061 cgccacctcc gcctgaggtc ggggtcgcgc aagtgatttc gaaaccggtc caacaatggg
     3121 acgaatacac gggccgcatc agcgcgatcg acacggtgga attacgcccc cgcgccagcg
     3181 gctacgtgca gcgagtggcg tacaaggaag gccaggatgt caaacagggc gacttgatgt
     3241 tcctgatcga tccccgtccc taccgggccg cactggaaaa cgcccaggcg cagctggcac
     3301 gcgcgcgtgt agcgcagcag ttggaaacca tccgcaacaa gcgcgcccag gctttgatcg
     3361 acgataacgc gatctcgcac gaagaactcg acttgcggcg cgcagcgcag gcgcaaagcg
     3421 cggcggatgt ccatgccgcc gaggctgcgg tggcgaccgc caagctcaac ctgtcgttca
     3481 cggaggtccg cgccccggtc tccgggcggg cgagccgggc gttggtgacc gtcggcaacc
     3541 tggcgac
//
LOCUS       DAOQDS010000145         3115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_24499_length_3115_cov_28.843137, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000145 DAOQDS010000000
VERSION     DAOQDS010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24499_length_3115_cov_28.843137"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PK589_18625"
     CDS             <1..365
                     /locus_tag="PK589_18625"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNJ21682.1"
                     /translation="GGKVLTLIDPTDVYMTVFLPAAEAGKIALGAQARITLDAAPNLV
                     IPAAVSFVADKAQFTPKEVETRSEREKLMFRIKVKIDPELVKGHEAQVKPGLPGVAYV
                     QLDSSVQWPPSLQTKLIR"
     gene            362..3109
                     /gene="rbbA"
                     /locus_tag="PK589_18630"
     CDS             362..3109
                     /gene="rbbA"
                     /locus_tag="PK589_18630"
                     /inference="COORDINATES: protein motif:HMM:NF033858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HNJ21683.1"
                     /translation="MSEPSASVARVATLTHRYGDTTALDAVTLDIPGGRMVGFIGPDG
                     VGKSSLLALIAGAKKIQVGRVEVLGGDMAAVRHRNEVYPRIAYMPQGLGGNLYLTLSV
                     FENVDFFGRLFGQNREEREWRITDLLRSTGLTPFRDRPAGKLSGGMKQKLGLCCSLIH
                     DPDLLILDEPTTGVDPLSRRQFWDLVDRIRKRRAGMSVLVATAYMEEAERFDWLVAMD
                     AGKVLATGSPAELKGRMNVRTLEDAFVSLLPEEKRRGHSAIAIPPRRTHEGPPAIEAH
                     GLTMRFGSFTAVDHVSFRIERGEIFGFLGSNGCGKTTTMKMLTGLLPASEGDARLFDR
                     PVNAQDMETRKRVGFMSQAFSLYGELTVRQNLELHARLFHLQPEKIPGRVAELTKRFK
                     LEDYLDDLAEALPLGIRQRLSLAVAVVHQPEILILDEPTSGVDPVARDGFWELLIELS
                     RKDRVTIFISTHFMNEGERCDRISLMHAGRVLVSDTPEGLIRTRGAANLEEAFIGYLE
                     EASGEATQKEVVEEAAETMPSASGTSRADRGRAFSPMRLFAYAHREALELRRDPIRLT
                     FALLGSVLLMFILGYGITMDVEDLRFAALDRDRTPQSRDYIQNLAGSRYFIERPFITD
                     DAELDRRMRSAELSVAIEIPSGFGRDLKRGRQTEVGVWVDGASPFRGETIKGYVQGMH
                     YQYLADLVRRTYGAQPQAGLADLEMRYRYNQDFKSLESMVPAVIPLLLVFIPALMTAL
                     GVVREKELGSITNLYVTPVTRLEFLLGKQLPYIALSMISFFGLVALAVVAFKVPLKGS
                     FATLTLGGVVYVIVTTGIGLLMSAFTRSQIGAVAGTAILTMLPTVQFSGLTDPVSSLE
                     GIGALIGQVWPATYFLVISRGTFTKGLDFWDLYGYLIALAVFIPVLTLLSLALLKKQG
                     T"
BASE COUNT          590 a          915 c          981 g          629 t
ORIGIN      
        1 caggaggaaa ggtcctgacg ctcatcgatc cgaccgatgt ctacatgacg gtgtttcttc
       61 cggccgctga ggcggggaaa atagcgctgg gtgcccaagc gcgaattacg ctcgatgccg
      121 ctccgaacct cgtcatcccc gctgcggtct cgtttgtcgc cgacaaggcg cagttcaccc
      181 cgaaggaagt ggagaccagg tcggagcgcg agaaactgat gtttcgaatc aaggtcaaaa
      241 tcgatcccga gttggtcaaa gggcatgagg cccaggtcaa accaggtctc ccgggcgtgg
      301 cctacgtaca actcgattcg tccgttcagt ggcctccatc cctgcaaacg aagctgatac
      361 gatgagtgaa ccatccgcat ccgtagcccg tgtcgccacg ttgacccatc gttatgggga
      421 cacgactgcc cttgatgcgg tgaccttgga cattccgggc ggccgcatgg tggggttcat
      481 cggtccggac ggagtcggca aatccagttt gctggccctg attgccggcg caaaaaaaat
      541 ccaggtcggc cgggtcgaag tcctcggcgg cgatatggcg gctgtgcggc atcgtaacga
      601 ggtctaccca cggatcgcct atatgcccca aggactggga ggcaacctct atctgacctt
      661 atcggtcttc gagaacgtgg atttcttcgg ccgcctgttc ggacagaacc gtgaggagcg
      721 cgaatggcgg atcacagacc tgctgcgaag cacggggttg acgccgtttc gggaccgtcc
      781 cgcggggaag ttatcaggcg gcatgaagca gaaactcggc ttatgttgct cgctgatcca
      841 cgatcccgat ctgttgattc tggatgaacc gaccaccggg gttgatccgt tgtcgcgccg
      901 gcagttctgg gacctcgttg atcggatccg gaagcggcgc gcggggatga gcgtgctcgt
      961 ggccaccgcc tacatggagg aggccgagcg attcgactgg ctcgtggcca tggacgcggg
     1021 caaggtgctt gcgacaggga gcccggcgga actgaagggt cgcatgaacg tacggacgtt
     1081 ggaagacgcc tttgtttcat tgctccccga agagaaacgt cgaggccaca gcgccatcgc
     1141 aatccctccc cgccggacac acgagggccc gccggcgatc gaagcgcatg gcctcacgat
     1201 gcggttcggc agttttacgg cagtcgatca tgtgagcttt cgcatcgagc ggggagaaat
     1261 tttcggtttt ctcggctcga acggctgcgg caagaccacg accatgaaga tgctcaccgg
     1321 cctgttgccc gcatcggaag gagacgccag gctgttcgac cggccggtga acgcccagga
     1381 tatggagaca cgcaagcgtg tcggcttcat gtcccaggcg ttctccctct atggtgaact
     1441 cacggtgcgg caaaacctgg agctgcatgc gcgcctcttc cacctccagc cggagaagat
     1501 ccccgggcgt gtggctgagc tgaccaaacg gttcaagctg gaagactatc tggatgatct
     1561 ggcggaggct ctgccgctcg gaatccgcca acgtctctcc ctggcggtcg ccgtcgtcca
     1621 tcagccggag atattgatcc tggacgagcc gacgtccggt gtcgacccgg tggcccgcga
     1681 cggattttgg gagctcttga tcgagctctc ccgcaaagac cgcgtgacga tcttcatctc
     1741 cacccatttc atgaacgagg gggaacgttg cgaccgcatc tcgctgatgc acgcggggcg
     1801 agtgctggtg agcgatacgc ccgaaggctt gatccgtacc cgtggcgctg cgaacctcga
     1861 agaggccttc atcggctacc tcgaagaagc aagcggagag gcgacgcaga aagaggtcgt
     1921 cgaggaagcc gcagagacaa tgccttccgc atcgggaacc tcaagagctg atcgtggcag
     1981 ggcgttcagc cccatgcggc tgttcgccta tgcccaccgc gaagccctgg agctgagacg
     2041 agaccccatc cggctcacgt ttgcgctgct gggatcggtg ctgctgatgt tcattcttgg
     2101 gtatggcatt accatggatg tggaagacct gcgcttcgcc gccctggacc gtgaccgaac
     2161 gccgcagagc cgggactaca ttcagaatct cgccggctca cgctacttca tcgagcgtcc
     2221 gttcatcacc gatgatgcgg aactggaccg gcggatgcgc agcgccgagt tgagcgtggc
     2281 gatcgagatc ccttcgggtt tcggccggga tttgaagcga gggcgccaga ccgaggtcgg
     2341 cgtgtgggtc gacggcgcca gtccgttccg gggcgagacc atcaaggggt acgtgcaggg
     2401 catgcactat cagtacctcg ccgacctggt tcgtcgcacc tacggcgctc agccacaggc
     2461 cggcctcgcc gatctcgaga tgcgctaccg ctacaaccag gacttcaaga gtctggaatc
     2521 gatggttccg gcggtgattc cgctgttatt ggtcttcatt ccggctctca tgacggccct
     2581 cggcgtggtg cgggagaaag agctcggctc catcacgaat ctgtatgtga cgccggtcac
     2641 acgcctggag tttctgctgg ggaaacaact gccgtacatt gctctcagca tgatcagctt
     2701 cttcggtctc gtcgcgctgg ccgtggtcgc gttcaaggtt cccctcaagg gcagctttgc
     2761 caccctgacg ttgggaggag ttgtgtatgt gatcgtcacg acgggaatcg gcttgctgat
     2821 gtccgcgttc acccgtagcc agatcggcgc agtggccggg acagcaatct tgacgatgct
     2881 gcccacggtc cagttttctg ggttgaccga tccggtgtcc tcgcttgagg ggatcggcgc
     2941 actcatcggg caggtctggc ccgcgacgta tttcctcgtg atcagtcgcg ggacctttac
     3001 caagggccta gacttctggg acctctatgg gtacctgatt gccttggcgg ttttcatccc
     3061 ggtattgact ctgctcagct tggcattgct gaaaaaacag ggcacatgaa gatga
//
LOCUS       DAOQDS010000146         2927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_27424_length_2927_cov_20.675487, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000146 DAOQDS010000000
VERSION     DAOQDS010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27424_length_2927_cov_20.675487"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1662
                     /locus_tag="PK589_18635"
     CDS             <1..1662
                     /locus_tag="PK589_18635"
                     /inference="COORDINATES: protein motif:HMM:NF015078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21684.1"
                     /translation="GNGACTGSGYRHMMIAVSRWIDRNILELARDFQLSYLPPIMVYM
                     AAGISGLTGIVGTFFVKDYLGLSAAFLAALGFWAGIPWALKMPFGHLVDLLWRWKSLL
                     VYFGAGVIAASLLIMVGLIGHREAMAAVMPAEVWYVLSALLAPIGYVIQDTVADAMTV
                     EAVPRVDEAGLPIDEAKLKLMHTTMQTLGRVAIISGGILVALINLYLFTGVENLPKDK
                     VAEIYQQVYLLALTIPAVSVLGVITASVLRLRAKRRFLSQGMTSAQVATLLDRPEEPT
                     KPNWWILGGSLLFVLFTLTVGMADFPFSEEIVFVGSMAIVSVLMVRLTRELAPDARHV
                     LVGTALVVFVFRAVPGPGEGATWWMIDKLGFDQHFLSVLSLIGSALTLAGMFLFRRFM
                     AERSIAYVVGFLTVAAFVLGLPIVSMYYGLHEWTAALTGGVVDARFIALVNTALESPL
                     GQISMIPMLAWIANSAPANLKATYFAVMASFTNLALSLSQLGTKYLNELFVVTREVRD
                     PVTNAIQTPDDYSQLGILLIVQTVLGLALPFAAILFSRFSRFRSA"
     gene            1683..2633
                     /locus_tag="PK589_18640"
     CDS             1683..2633
                     /locus_tag="PK589_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNJ21685.1"
                     /translation="MHTFDFLAEHGALVLFWVIFVEQIGFPLPALPLLVAAGALVATG
                     NMSLAAALLVPVAASLPPDLAWYYLGRQKGGKVLGFLCRLSLEPDSCVRDTENVFHRN
                     GPRALLLAKFVPGFSTVAPPLAGIVGMPATTFILYDLGGTLIWAAVSAGVGALFSNQL
                     EQLIGLFDQAGGLMLVILLLGLAGFVGYKFYHRQKFLRHLRMAKISVDELKRRLDAGE
                     SISVVDVRHPLALDLDPESIPGAINFTLEDIEHRHHEIPRDRDIVLYCSCPNEVSSAR
                     TAFLLKKKGIHRVRPLEGGLDAWRERQFPVERRVNIELTQ"
     gene            2665..>2927
                     /locus_tag="PK589_18645"
     CDS             2665..>2927
                     /locus_tag="PK589_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21686.1"
                     /translation="MPMTGLALPVIAGIVLLSGGAETSAGRDWKTGSDTYAIREVKSP
                     SRQKAAGVTYKVVGVFQRDKQFIGGSAVADVTEQVEFEFTWPLV"
BASE COUNT          486 a          831 c          907 g          703 t
ORIGIN      
        1 ggcaatgggg cctgcaccgg ttctggttac cgccatatga tgatcgccgt ttcccgctgg
       61 atcgaccgta atattctgga actcgcccgc gacttccaac tatcctattt gcctcccatc
      121 atggtctaca tggcggccgg catttccggg ctcaccggta tcgtcggcac gttcttcgtg
      181 aaagattatc tcggcctttc cgccgccttt ctcgccgccc tgggattctg ggccggtatc
      241 ccctgggcgc tgaagatgcc attcgggcat ctggtcgatc tcctgtggcg atggaagagc
      301 ctcctcgtct atttcggtgc gggcgtcatt gcggccagtc tgctgatcat ggtcggcttg
      361 atcggtcatc gcgaggcaat ggcggccgta atgccggcgg aagtctggta tgtgctgagc
      421 gcgctgctcg cccctatcgg ctatgtgatt caagacacgg ttgctgatgc catgacggtg
      481 gaggcggtcc cgagagttga cgaggcaggg cttcccattg atgaagccaa gctgaagctc
      541 atgcacacga cgatgcagac gctcggccgt gtggccatca tcagcggcgg gattctggtc
      601 gccctcatca atctgtatct gttcacgggt gtcgagaatc tgccaaaaga caaagtcgct
      661 gagatttatc agcaggtcta tctcctggcg ctgactatcc cggccgtgtc ggtactcgga
      721 gtcatcaccg cgtcggtgct tcggctgcga gccaagcgcc ggtttctcag ccagggcatg
      781 acatctgctc aagttgccac gctgctggac aggcctgagg aaccaaccaa gcccaattgg
      841 tggattctcg gaggcagcct gctgttcgtc ttgtttaccc tgacggtggg catggcggat
      901 tttccgttca gcgaagaaat cgtcttcgtc ggatcgatgg ccatcgtctc ggtcttgatg
      961 gtgcggctga ctcgcgaact ggctcccgac gccaggcatg ttctcgtggg cacggctctg
     1021 gtggtgtttg tctttcgcgc ggtgccggga cccggcgaag gcgcgacctg gtggatgatc
     1081 gacaagctgg gcttcgatca acattttctt tcggtgttat cgctcatcgg cagtgccttg
     1141 accctggccg gcatgtttct ctttcggcga tttatggccg aacggtccat tgcctatgtc
     1201 gtcggctttc ttaccgtggc ggcctttgtc ctcgggctcc ccatcgtcag catgtattac
     1261 gggctgcacg aatggaccgc ggcgctgacc ggcggtgtag tggatgcgcg gtttatcgcc
     1321 ttggtgaata cggcgctcga atcgcccctc ggccagatct ccatgattcc catgttggcc
     1381 tggattgcga actccgcgcc ggcgaatttg aaggccacgt atttcgccgt catggcctcc
     1441 ttcaccaatc tggcgttgtc gctcagccag ctcgggacga aatatctcaa cgagctcttt
     1501 gtggtgactc gggaagtgcg cgatccggtc accaacgcca ttcagacgcc tgatgattac
     1561 agtcagctgg gaatattgct gattgtgcaa acggtgttgg gcctcgcgct cccctttgcg
     1621 gctattctct tttcgcggtt ctcgcgtttc cggagtgcct aagcgggagg acgtcatccg
     1681 ctatgcatac gttcgacttt ctcgccgagc atggggcctt ggtgctcttc tgggtgatct
     1741 tcgtcgagca gatcgggttt ccgctccctg cgcttccact cctcgtggcc gcaggcgcgc
     1801 tggttgcgac aggaaacatg agtctggcgg cggcgttgct ggtgccggtt gctgcttctc
     1861 ttccgccgga tcttgcctgg tattacctgg gtcgtcagaa gggcgggaag gtgctgggct
     1921 ttctctgccg gctgtcgctg gaacccgatt cctgtgtgcg ggatacggaa aatgtgtttc
     1981 accggaacgg ccctcgcgca ttgctgttgg ccaaatttgt tcccggcttc agcacggtgg
     2041 cgcctcccct cgccggcatt gtcggcatgc ccgccacgac gtttatcttg tacgatctcg
     2101 gcggcacgct gatttgggcg gcggtcagcg ccggagtcgg cgcgttgttc agcaatcagc
     2161 tggaacagct catcggacta tttgaccagg cgggaggact gatgctcgtg atccttctgc
     2221 tgggtctggc gggattcgtc ggctataagt tttatcaccg gcagaaattc ctgcggcatc
     2281 tgcgcatggc gaaaatttcc gtcgatgagc tcaagcggcg tctggatgcc ggggagtcca
     2341 tcagcgtggt cgacgtccga catccgctcg cccttgatct ggatcccgaa agcattcccg
     2401 gcgctatcaa ctttacgttg gaagacatcg agcatcggca tcacgagatc ccgcgcgatc
     2461 gcgacatcgt gctctactgc agctgcccga atgaagtctc cagcgccaga acggcctttt
     2521 tgctcaagaa gaaaggcatt caccgggtga ggcctctgga aggcgggctg gatgcctggc
     2581 gggagcgtca gtttcccgtc gagcggcggg tcaatatcga gctgactcag tgagtctgtg
     2641 gaatcttcag gcgaggaaag gacgatgcct atgacgggtc tggcgcttcc agtgatcgca
     2701 gggatcgtcc tgctctccgg cggggcggag acgagcgcag ggagagactg gaagacgggt
     2761 tccgatacct acgcgattcg ggaggtgaag agtccctcgc gccagaaagc cgcaggcgtg
     2821 acgtacaagg tcgtgggagt cttccagcga gacaaacagt ttatcggggg atctgccgtg
     2881 gccgatgtga ccgagcaggt ggaatttgaa tttacctggc cgctggt
//
LOCUS       DAOQDS010000147         2669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0750
            NODE_32526_length_2669_cov_16.884086, whole genome shotgun
            sequence.
ACCESSION   DAOQDS010000147 DAOQDS010000000
VERSION     DAOQDS010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810382
            Sequence Read Archive: SRR3501884
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:17:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,732
            CDSs (total)                      :: 3,696
            Genes (coding)                    :: 3,687
            CDSs (with protein)               :: 3,687
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32526_length_2669_cov_16.884086"
                     /isolate="AMR_MDS_0750"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PK589_18650"
     CDS             <1..258
                     /locus_tag="PK589_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21687.1"
                     /translation="RFERLGRDRHYNIIFHIGTSYVPVSDETIDELKAQSLLSSERFL
                     DLLIDKIGYTNYLKEQIRGELQASGDPMTQMTVLQGAIREL"
     gene            703..2178
                     /locus_tag="PK589_18655"
     CDS             703..2178
                     /locus_tag="PK589_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21688.1"
                     /translation="MLQEIIESPTATMRPATEHMSSPSASMTVRVGFVPTPTREYRQH
                     RKLLRATTGIASRASVRMMPDRESARVVIPIPIPIPRIIGSRVLMWKQDPSVSEIGIR
                     KAFLPGLLLAGPRDSRISIQGMPLVSPNAMADYIETPGTQEFDAVHTFAVVRQMLTLA
                     QRARGGSVAPWQWNSASNTEGMSVFPRAGVTPNAFYSRNLKALKFFFFTKPGSPPGSP
                     QVFTCRSFDIVAHETGHAVLDGLKPGWLDASNHPQTGGLHESFGDLCAIFLTLSQLDQ
                     VEAIIAQTKANLHDKTFLADMAEEFGLALGRPNGLRNADNDLKLSEVTNEVHDLSQVF
                     TGAIYDVLADIFAFERKPGMRDDARVLLEVAEYLFGLILRAINAAPAANATFADMANQ
                     MLTIAHAEGKPVQYRNFIRNRFTVREIVVSPTPLTADHKAGMKLMPAFTEEIRMSQDR
                     RGCCGTMQLPEYNEGDRVFEDDLEELRNLFSDNGRAKVLHR"
     gene            2253..2546
                     /locus_tag="PK589_18660"
     CDS             2253..2546
                     /locus_tag="PK589_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ21689.1"
                     /translation="MKITIQRTGGFAGLQEALASVDTDQMPAAEARRVEELVAKAAFF
                     DLPAIVGGNIKGADVYRLRVTVTDKSRHQSVEFPDHDSPELTSLRALVRSLAP"
BASE COUNT          587 a          828 c          715 g          539 t
ORIGIN      
        1 cggttcgaac gactcgggcg cgatcgccac tacaacatca ttttccacat cggcaccagc
       61 tatgtgccgg tgagcgacga gaccatcgac gaactgaagg cgcaaagtct gttgtcctcc
      121 gaacgttttc tcgacctcct catcgataaa atcggctaca ccaactatct caaagaacag
      181 atccgggggg aactccaggc ctccggcgac cccatgactc agatgaccgt cctacaaggc
      241 gccattcgcg aactgtagat ccctgctcct tcgctccaca gtttcatcca tcgaccatat
      301 acttgttccc tgaacaccct cgtgtcagga catctcctgc acgtccacgg tacatgctcc
      361 gttgaaacac cttgccctgt acggaaatag atacgcccaa gccttggctg tatctcctga
      421 gatacgccta ggtagaaacc ggcaaacaat gccaccatta ccaaccagct gaaatcccga
      481 ttctcgtttc gaagcgccgg agacgaccgg tcacagaatc gagacggtac gacacttgct
      541 ttttcttttg cggggaagga ggtgagtcgc atgtccactc tgtcatccaa cggacgaacc
      601 agccaggtca cgcaaaagca ttctccctca catcgagtcg gtgcctccaa tccaaaatcc
      661 agcgcggccg ctatcgactc agaaaggaca agggtaagag ccatgcttca ggagatcatt
      721 gaaagtccga cggcgaccat gagaccagcc accgaacaca tgagtagccc ttcggccagc
      781 atgacggttc gcgtcgggtt cgttccgact ccgacacgtg aatatcggca gcatcgaaaa
      841 ctcttgcgtg ctaccaccgg catcgccagc cgtgcctccg ttcgcatgat gcccgaccgg
      901 gaatcagccc gggtggtcat cccgatcccg attcccatcc cccggatcat cggctctcga
      961 gtgctgatgt ggaagcagga cccctcggtc agcgaaatcg ggattcgcaa ggcctttctc
     1021 cccggtctgt tattagcggg cccgagagac agccgcattt ccattcaagg catgcccctc
     1081 gtctccccca acgccatggc ggactatatt gagactccag ggacgcagga gtttgatgcg
     1141 gtccacacct ttgctgtcgt ccggcaaatg ctcactctcg cgcaacgggc acgtggcgga
     1201 tccgtggcac cgtggcaatg gaacagcgcc tccaacacgg aagggatgag cgtctttccg
     1261 cgggccggcg tcactcccaa tgcgttctac agtcgcaatc tcaaagcctt gaaattcttc
     1321 ttcttcacca agccgggcag ccccccgggg tctccccagg tgttcacctg ccgctcgttc
     1381 gatatcgtgg cgcatgaaac cggccacgcc gtcctggatg gccttaagcc agggtggctc
     1441 gacgccagca atcatccgca gaccggtgga ttgcatgaat cgttcggcga cctctgcgcg
     1501 atctttctga cgctttccca actcgaccag gtggaggcga tcatcgccca gaccaaagcg
     1561 aacttgcatg acaagacatt ccttgccgac atggccgagg aattcggcct tgcactcggc
     1621 cgcccgaacg gtctccgcaa tgcggacaat gatttgaagc tgagcgaggt gaccaacgaa
     1681 gtgcacgatc tgtcacaggt cttcacggga gccatctacg atgtgctcgc agatatcttc
     1741 gcgtttgagc gaaaacccgg gatgcgggac gacgccaggg tcctgctcga agtcgcggag
     1801 tatctgtttg gcctgatcct ccgagccatt aacgccgctc cggccgcaaa tgccaccttc
     1861 gccgatatgg ccaaccagat gctgacgatt gcgcacgcag agggcaagcc ggtccagtac
     1921 agaaacttca ttcgcaaccg cttcacggtt cgggaaatcg tcgtctcgcc gacgccgctc
     1981 accgccgacc ataaagcggg tatgaaactc atgccggcgt ttacggaaga aatccggatg
     2041 agccaagacc ggcgaggctg ctgcggaacc atgcagctcc ccgaatataa tgagggggac
     2101 cgtgtattcg aagacgatct ggaggaactc cggaaccttt tcagcgacaa tggaagagcg
     2161 aaggtcctcc atcgataaga tcagtctcaa tgaggcggct cgggaggcca tacggtgccc
     2221 gggccgccat tgagctccac tgaggacagg cgatgaagat cacgattcaa cgcaccggtg
     2281 ggttcgccgg gctccaagag gcactggctt ctgtcgatac ggatcagatg ccggctgcgg
     2341 aggcgaggcg cgtcgaagaa ttggtcgcga aagctgcgtt ctttgatcta ccggccatag
     2401 tcggcggcaa catcaaaggc gcagacgtct accgtctgcg agtaactgtt acagataaat
     2461 cgcgtcacca gagcgtcgaa tttcctgatc acgactcccc tgaactgacc tccctgagag
     2521 ctctcgtccg gtccctggct ccctagtcga ccgtgggtcg tgtgtgggac ggccgggctt
     2581 cctacaagct cttcgatttc actaatcccc ggtctgaccg ctggcgtgga gagcgttcat
     2641 gatggcattt ttcttggcgg gatcttttt
//
